LOCUS       DAYAOX010000001        55885 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.001, whole genome shotgun sequence.
ACCESSION   DAYAOX010000001 DAYAOX010000000
VERSION     DAYAOX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.001"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..1206)
                     /locus_tag="ACGI57_000001"
     CDS             complement(<1..1206)
                     /locus_tag="ACGI57_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFG9715093.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNS"
     gene            1669..2865
                     /locus_tag="ACGI57_000002"
     CDS             1669..2865
                     /locus_tag="ACGI57_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFG9715094.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            2958..3482
                     /locus_tag="ACGI57_000003"
     CDS             2958..3482
                     /locus_tag="ACGI57_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFG9715095.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            3507..3683
                     /gene="rpmF"
                     /locus_tag="ACGI57_000004"
     CDS             3507..3683
                     /gene="rpmF"
                     /locus_tag="ACGI57_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFG9715096.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            3890..4447
                     /gene="fapR"
                     /locus_tag="ACGI57_000005"
     CDS             3890..4447
                     /gene="fapR"
                     /locus_tag="ACGI57_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HFG9715097.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            4458..5480
                     /gene="plsX"
                     /locus_tag="ACGI57_000006"
     CDS             4458..5480
                     /gene="plsX"
                     /locus_tag="ACGI57_000006"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFG9715098.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            5506..6492
                     /locus_tag="ACGI57_000007"
     CDS             5506..6492
                     /locus_tag="ACGI57_000007"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFG9715099.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            6601..7530
                     /gene="fabK"
                     /locus_tag="ACGI57_000008"
     CDS             6601..7530
                     /gene="fabK"
                     /locus_tag="ACGI57_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HFG9715100.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            7546..8496
                     /gene="fabD"
                     /locus_tag="ACGI57_000009"
     CDS             7546..8496
                     /gene="fabD"
                     /locus_tag="ACGI57_000009"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFG9715101.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            8496..9245
                     /gene="fabG"
                     /locus_tag="ACGI57_000010"
     CDS             8496..9245
                     /gene="fabG"
                     /locus_tag="ACGI57_000010"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFG9715102.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            9299..9523
                     /gene="acpP"
                     /locus_tag="ACGI57_000011"
     CDS             9299..9523
                     /gene="acpP"
                     /locus_tag="ACGI57_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFG9715103.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            9552..10790
                     /gene="fabF"
                     /locus_tag="ACGI57_000012"
     CDS             9552..10790
                     /gene="fabF"
                     /locus_tag="ACGI57_000012"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFG9715104.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADLMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            11016..12734
                     /locus_tag="ACGI57_000013"
     CDS             11016..12734
                     /locus_tag="ACGI57_000013"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9715105.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            12765..14765
                     /locus_tag="ACGI57_000014"
     CDS             12765..14765
                     /locus_tag="ACGI57_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9715106.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            15116..15361
                     /locus_tag="ACGI57_000015"
     CDS             15116..15361
                     /locus_tag="ACGI57_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HFG9715107.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            15396..16121
                     /locus_tag="ACGI57_000016"
     CDS             15396..16121
                     /locus_tag="ACGI57_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFG9715108.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            16143..17456
                     /locus_tag="ACGI57_000017"
     CDS             16143..17456
                     /locus_tag="ACGI57_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9715109.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            17662..18201
                     /locus_tag="ACGI57_000018"
     CDS             17662..18201
                     /locus_tag="ACGI57_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFG9715110.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            18290..20275
                     /locus_tag="ACGI57_000019"
     CDS             18290..20275
                     /locus_tag="ACGI57_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HFG9715111.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            20499..21467
                     /gene="spoIIIAA"
                     /locus_tag="ACGI57_000020"
     CDS             20499..21467
                     /gene="spoIIIAA"
                     /locus_tag="ACGI57_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HFG9715112.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            21457..21978
                     /locus_tag="ACGI57_000021"
     CDS             21457..21978
                     /locus_tag="ACGI57_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HFG9715113.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            21995..22192
                     /gene="spoIIIAC"
                     /locus_tag="ACGI57_000022"
     CDS             21995..22192
                     /gene="spoIIIAC"
                     /locus_tag="ACGI57_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HFG9715114.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            22217..22594
                     /gene="spoIIIAD"
                     /locus_tag="ACGI57_000023"
     CDS             22217..22594
                     /gene="spoIIIAD"
                     /locus_tag="ACGI57_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HFG9715115.1"
                     /translation="MQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVMFKL
                     NFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEFGGK
                     IIVMSMSFPILLSIVEMVVNIIP"
     gene            22679..23884
                     /gene="spoIIIAE"
                     /locus_tag="ACGI57_000024"
     CDS             22679..23884
                     /gene="spoIIIAE"
                     /locus_tag="ACGI57_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HFG9715116.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            23944..24561
                     /locus_tag="ACGI57_000025"
     CDS             23944..24561
                     /locus_tag="ACGI57_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HFG9715117.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            24573..25127
                     /locus_tag="ACGI57_000026"
     CDS             24573..25127
                     /locus_tag="ACGI57_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HFG9715118.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            25154..25843
                     /locus_tag="ACGI57_000027"
     CDS             25154..25843
                     /locus_tag="ACGI57_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HFG9715119.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNVDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            25953..26315
                     /locus_tag="ACGI57_000028"
     CDS             25953..26315
                     /locus_tag="ACGI57_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFG9715120.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            26400..26906
                     /gene="nusB"
                     /locus_tag="ACGI57_000029"
     CDS             26400..26906
                     /gene="nusB"
                     /locus_tag="ACGI57_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFG9715121.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            26890..27960
                     /locus_tag="ACGI57_000030"
     CDS             26890..27960
                     /locus_tag="ACGI57_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HFG9715122.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            27957..29162
                     /gene="xseA"
                     /locus_tag="ACGI57_000031"
     CDS             27957..29162
                     /gene="xseA"
                     /locus_tag="ACGI57_000031"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFG9715123.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            29164..29379
                     /gene="xseB"
                     /locus_tag="ACGI57_000032"
     CDS             29164..29379
                     /gene="xseB"
                     /locus_tag="ACGI57_000032"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFG9715124.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            29382..30269
                     /locus_tag="ACGI57_000033"
     CDS             29382..30269
                     /locus_tag="ACGI57_000033"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFG9715125.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIGKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            30299..30736
                     /locus_tag="ACGI57_000034"
     CDS             30299..30736
                     /locus_tag="ACGI57_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HFG9715126.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            30770..32635
                     /gene="dxs"
                     /locus_tag="ACGI57_000035"
     CDS             30770..32635
                     /gene="dxs"
                     /locus_tag="ACGI57_000035"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFG9715127.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            32768..33580
                     /locus_tag="ACGI57_000036"
     CDS             32768..33580
                     /locus_tag="ACGI57_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HFG9715128.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            33600..35279
                     /gene="recN"
                     /locus_tag="ACGI57_000037"
     CDS             33600..35279
                     /gene="recN"
                     /locus_tag="ACGI57_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFG9715129.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            35493..36359
                     /locus_tag="ACGI57_000038"
     CDS             35493..36359
                     /locus_tag="ACGI57_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715130.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(36452..36943)
                     /locus_tag="ACGI57_000039"
     CDS             complement(36452..36943)
                     /locus_tag="ACGI57_000039"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9715131.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(37004..37444)
                     /locus_tag="ACGI57_000040"
     CDS             complement(37004..37444)
                     /locus_tag="ACGI57_000040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9715132.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            37605..38663
                     /locus_tag="ACGI57_000041"
     CDS             37605..38663
                     /locus_tag="ACGI57_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HFG9715133.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            38805..39629
                     /gene="spo0A"
                     /locus_tag="ACGI57_000042"
     CDS             38805..39629
                     /gene="spo0A"
                     /locus_tag="ACGI57_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA];
                     GO_process: GO:0051606 - detection of stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HFG9715134.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            40042..41163
                     /gene="steA"
                     /locus_tag="ACGI57_000043"
     CDS             40042..41163
                     /gene="steA"
                     /locus_tag="ACGI57_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HFG9715135.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            41181..42056
                     /locus_tag="ACGI57_000044"
     CDS             41181..42056
                     /locus_tag="ACGI57_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HFG9715136.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            42080..42772
                     /locus_tag="ACGI57_000045"
     CDS             42080..42772
                     /locus_tag="ACGI57_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9715137.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            42780..43604
                     /locus_tag="ACGI57_000046"
     CDS             42780..43604
                     /locus_tag="ACGI57_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFG9715138.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKNLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIVFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            43609..44676
                     /locus_tag="ACGI57_000047"
     CDS             43609..44676
                     /locus_tag="ACGI57_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HFG9715139.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            44678..45214
                     /locus_tag="ACGI57_000048"
     CDS             44678..45214
                     /locus_tag="ACGI57_000048"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFG9715140.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            45282..45887
                     /locus_tag="ACGI57_000049"
     CDS             45282..45887
                     /locus_tag="ACGI57_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HFG9715141.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKSR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            45899..46813
                     /locus_tag="ACGI57_000050"
     CDS             45899..46813
                     /locus_tag="ACGI57_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9715142.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAAEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            46855..48027
                     /locus_tag="ACGI57_000051"
     CDS             46855..48027
                     /locus_tag="ACGI57_000051"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFG9715143.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIEGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            48074..48886
                     /locus_tag="ACGI57_000052"
     CDS             48074..48886
                     /locus_tag="ACGI57_000052"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFG9715144.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            49090..50415
                     /locus_tag="ACGI57_000053"
     CDS             49090..50415
                     /locus_tag="ACGI57_000053"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFG9715145.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            50628..51707
                     /gene="mltG"
                     /locus_tag="ACGI57_000054"
     CDS             50628..51707
                     /gene="mltG"
                     /locus_tag="ACGI57_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFG9715146.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFSYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            51879..52553
                     /locus_tag="ACGI57_000055"
     CDS             51879..52553
                     /locus_tag="ACGI57_000055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFG9715147.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRKYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            52540..53787
                     /locus_tag="ACGI57_000056"
     CDS             52540..53787
                     /locus_tag="ACGI57_000056"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFG9715148.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEIYEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            53859..55529
                     /locus_tag="ACGI57_000057"
     CDS             53859..55529
                     /locus_tag="ACGI57_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFG9715149.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDIDKSMKKELEKNGIMVEEKTMRYAKDGL
                     LSHTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKT
                     VDNKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQND
                     ISKSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNT
                     NEILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGL
                     DEEKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNM
                     NTIKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLN
                     KKAIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(55626..>55885)
                     /locus_tag="ACGI57_000058"
     CDS             complement(55626..>55885)
                     /locus_tag="ACGI57_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9715150.1"
                     /translation="ILGTEANYVLDEVELKVQVGKLYEQLNKILTPREREIVQLRYGL
                     TPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
BASE COUNT        22584 a         5666 c        10331 g        17304 t
ORIGIN      
        1 tgaattatat atatttgtag cttttatatc ataacttatg agtgttttaa aaatcgtatc
       61 atctaatgaa aatgatatat ttgaaaactt gtttattatt tttatttcag atttattttt
      121 tatctctttt agaatttctc tacctttatc attaaaagca agtattctta tataaggtat
      181 agtatctatg ttttttataa gttgcacgtc gcttttttta atacctaaca atatattgtt
      241 tagagttcgt ttgattttgg ttaaagtgta tcgcttggat ttaattgcta attgtagttc
      301 atgtagaaat tcagatgtaa agatatcttt atagattctg ttttctatgc cttcatttac
      361 ttcaaaatac tcttttaaag tgtccatatc tctaagtata attgttttta aaatatcaaa
      421 gaagaagtca tcaaacatag gtaaaaaacc attatctatt ttttcattta ggatgttata
      481 agttttttct ggaataacat tttttaaatg aaaaaggtct gtattttctt taagtgaagt
      541 tcttatagca gtagcagaac aaatatcact ctcaatttct tcggaattat attctgattg
      601 gaccctggag atagtaaatg gttttatact actgtttaaa tggatgaggt tttttatgta
      661 ttctataccc agtatgttgt ttgatgaatt taatgttttt gataattgtt cctttgaata
      721 attttgtata aaattatttt ttttgtcttg atgtatataa tcaaaaagtg cattagctct
      781 agctgttgga aataacagac catcatctaa atattttttt aaaagtaact taaattcatt
      841 tggttcattt gctaaaattt cagctagatt ataaagaatt tctgtatttc cttcttcact
      901 accaaaacat atagaattta tgcagtttag tgaatttaaa attgttatag caccatgaga
      961 aaaaatttca gcactttgac aagcaaataa agttggtaat tctataacta agtctacacc
     1021 attttcgaca gcaatttttg ctctagtgta tttatcaaat aaagctggtt cgcctctttg
     1081 tagaaaattt ccactcatta ttgctacagt atgtgttgca tttgtttttt ctatagattt
     1141 agataaatga tatatgtgtc cattatgaaa tgggttatat tcaactatta atccaagtat
     1201 gttcataaaa tactcctttt atctttaatt attttatata tatcacaatt tattataata
     1261 tgtataaaat ttaaattttt aattataaaa ttaaaaaacg taaaaaaatt tataaaaatg
     1321 tagaaatgtt acctacataa aaaactggtt aaatgttatt aaaataactt tattagctct
     1381 ttatattata ttattataac aaatgggaaa gaataatcta cattaagtta acttgttaat
     1441 atattattag tgaaatacac aatttaccat actagaaata tgatataata taaaaaaaaa
     1501 gtatatactt ttatattaaa aggtgatata ttatttatga aaatcgttta atgtatacaa
     1561 taatatatct ataacaaaat aataaaagat agagttttat ttagacattt attacaaatc
     1621 aaaatttatt acatattatt tataaaaaaa tctaaggagg aaataataat gaaaatatta
     1681 gtattaaact gtgggagttc ttcattaaaa tatcaattaa tagatatgaa taatgaagaa
     1741 gttttatgta taggattggt tgaaagaata ggaatagaag gttctatatt aaaacatgaa
     1801 aaagcaggta gagatgataa atacgttgtt gaacaaccaa tgaaggacca caaagatgca
     1861 atagcattag ttttagaagc tgttgctcat ccagaatttg gtgcagttaa agaaatgaaa
     1921 gaaatagatg cagtaggaca tagggttgta catgctggag aaaaatttgc aacttcagta
     1981 gtaataactc cagaagtaga agaagcttta aaagaatgta tagatttagc tccacttcat
     2041 aatccagcaa acataatggg aatagatgct tgtaaagcta tacttccaga tgtaccaatg
     2101 gtaggtgtat ttgatactgc tttccatcaa acaatgccta aatcttcata cttatatggt
     2161 ttacctcatg agctatatac taaatatggg gtaagaagat atggattcca cggaacttct
     2221 cataattatg tatctcaaag agcagcagag atattaggaa aagatataaa agatttaaaa
     2281 atagtaactt gtcacttagg aaatggtgct tctatagctg ctgttgatgg tggaaaatgt
     2341 gtagatacat ctatgggatt cactccatta gaaggattaa taatgggaac tagatgtgga
     2401 gatatagacc cagctatatt accattctta atgagaaaag aaggtttaga tgctgatgga
     2461 ttggataaat taatgaataa agaatctgga gtatatggaa tgactggaat ttctagtgac
     2521 ttcagagata tagaagatgc agctaaaaat ggagacgaaa gagctcaagc tacattagaa
     2581 gcgtatgtta aaaaagtaca aaaatatata ggcgcttatg ctgctgaaat gaatggattg
     2641 gatgttgtag tatttactgc aggcgttggt gaaaatggaa aggctataag agcagatatt
     2701 gcttctaaca tggagttttt aggaatgaaa ttagataaag aagctaatga tgttagagga
     2761 aaagaaacag taatatctac tgctgattct aaagtaaaaa tgcttttaat accaactaat
     2821 gaagaattaa tgatagctag agatacatta agattggtaa aataattaaa ttaaacctat
     2881 gcctatcaag taaaaatact tgacaaacaa atgctagatt tgtataataa atttggtgat
     2941 atgttgaggt gaacttaatg aaagttagca tagaaaaaat aaatagaaaa gaaactgaca
     3001 aaatagactt gaatttttgt gaaaaaattg atactattag ttattgtgat gagatttaca
     3061 aattagtatc acctgtaaat ttaaaaggga aagtgtcaaa gactaataaa ggtttgtatc
     3121 tagatataga tgtcaatttt acaattgttg acaattgttc aagatgtctt aaagaagtag
     3181 aaataccact agagtattct atacaaggtt ttttagtaaa agaagaagat tatgatgaag
     3241 atgagtttga ggaatttgat ccttttatat tcgatggtga agaaatcgac cttattgata
     3301 taatagaaca gacattagat tttaatgtac ctcataaagt tctttgtagt gaaaactgta
     3361 aaggtctttg tcaagtatgc ggggcaaact taaatgaaga agagtgttct tgcagtgaaa
     3421 ttacaaacga tgaggaatac atagatcctc gttttgctaa attaaaagat ttatttaatt
     3481 aaattttgac taaggaggtg gcgtaaatgg cagtaccaaa gcgtaaaacg tctaaatcaa
     3541 acacaaaaat gagaagagca gctaactcaa agatggaagc aacaggattt gtaagttgcc
     3601 cacaatgtca tgagccaaaa ttaccacata gagtgtgtcc agactgtgga tattataaag
     3661 gtaaagaagt tgtgtctaag taatagcttc taaaaagcat gtagagtata ctctacatgt
     3721 tttttttgta catataaaaa aataagataa tttactagag aaaataagtg gattttatgc
     3781 atattaaaaa atagaaaaat aataatataa gtattgaaaa tttttgtgtg ttactatata
     3841 ctactattag tagctggtac taattacagc tataaaattt ggagggaaca tgaaaaagaa
     3901 aagtaaagcc caaagacaaa aagagcttat agatatgcta aagacagatc ctttttatac
     3961 tgatgaagaa ttatcaagtc tgtttgatgt aagtatacag accataagat tagatagaat
     4021 gagtctaaac ataccagagc taagagaaag agtcaaatca atagcagaaa ctcagagttc
     4081 aaaagtaaag actttaggag taaaggaaat tacaggagaa atcattgatt tatctgtagg
     4141 gcgattaggt atatctatgt tagaagttac acaggacatg atttactcta agactaatac
     4201 tttaaaagat acgtatattt tttctctagc agattcattg gcaatggcta taatagatgc
     4261 acctaaggtt attatgagag ttgcaaatgt aaaaagtttt aagttgatag aacaacaaga
     4321 taggcttata gcaaaagcag aagtgtatag aaatatagat aaaaagcatt atgtaaaagt
     4381 tgttatcaac aataaagccc aagagcaaat atttagagga aagtttatct ttgaagaatt
     4441 agattaggag ggaaattatg aaaatagtaa ttgatggaat gggtggagat aatgcaccta
     4501 aatctaatgt agaaggcgca gtaaatgcta taaaagaata tcaggttgat ttaataatca
     4561 caggagataa agatttactt gaaaaggaat tttcaaatta tgaatttgat agaaataaat
     4621 tagagatagt tcatactact gagattatag aaaacgaaga taaaccagta aaggctataa
     4681 gaagtaaaaa agattcatct atggtagtag cattaaatct tgtaaaagaa ggaaaggcag
     4741 atgctataat atcagctgga aatacaggag ccttacttgc tggtggatta tttgttgttg
     4801 gtagaataaa aggaatcgat agaccttgct tatgttcagc aataccaaat gtaaagcgag
     4861 gaatgacact tatagcagac tgtggcgcaa atgcagattg taaacctaag aatctagttg
     4921 aatttgcagc tatgagtaat atttattcaa gaaaagtttt aggtcttgaa aatcctaagg
     4981 tagcattagc aaatgttgga ctagaagaag gcaaaggcaa tgatttagtc aaaaggtcat
     5041 atgaagaaat taaaaaatta gatttaaatt ttatagggaa tgtagaagct agagaagtta
     5101 taaatgcata cacagatata atcatatgtg atggatttac aggtaatata ttacttaaat
     5161 ctgctgaagg tgtagcactt tcagttatga gtcttataaa ggaaactttt atggccagta
     5221 caaaaagtaa aataggagca ttacttataa aggatgattt aagaaaatta aaaagtttta
     5281 tagattattc tgagtatggg ggagctccac ttttaggtct aaatggagga gttataaaag
     5341 ctcatggtag ttctgatgct aaagctataa aaaatgctat aaatcaagga ataaaatttt
     5401 caaagggaaa agtcgttgaa gatattaacc aatttataag caaatacaat gaagaaaata
     5461 aaaataacga agatgaataa actcgttttg gaggtttaat atataatgaa tacaaaggct
     5521 ggaatattag gagtaggaag ttatctacct gagcaatcat atgataattt tcacttcgaa
     5581 aaaataatgg atacatcaga tgagtggata agtactagaa ctggtattaa ggaaagaaga
     5641 tttgccaaag agtctgaagc aacttctgac ttagcatcta aagctgcctt aaaagctata
     5701 gaatgtgcaa agttaaacgt agaggatata gagctgatta tattagctac aattactcct
     5761 gatatgtctt tgccttcaac tgcatgtata gtacaagatg caataggagc agtaaatgct
     5821 actgcatttg atatatcagc agcttgttct ggatttgtat atggagtaac tattgcaaag
     5881 caatttgtgg aaacaggttg ctataaaaat gtgcttgtaa taggagcaga aacttgttct
     5941 aagtttttaa attatgatga tagaacaaca gcagtattgt ttggagatgg agctggagca
     6001 gctgttattg gccctgtaaa tgagggtgga atactgtcta cacatatggg ctctgatggt
     6061 aaaggaaaag attgtttaaa agtacctgct ggaggttcaa gacttaaggc aagtaaagag
     6121 actgtagaag ctaatctaca tacaatagaa atggcaggta gtgatgtgtt taaatttgca
     6181 gttagaaaga tggcagaaac ttctttaaga gctcttgaaa aagcaaatct aaatacaact
     6241 gatatagact acttagtgcc acatcaagct aatataagaa tcatacaagc ttcttctaag
     6301 agattagagc ttgatatgaa aaaagtttat gtaaatatag acaaatatgg aaatatgtct
     6361 gctgcatcta taccagtagc attagatgaa gcttatagag aaggtaaaat taaaaagggt
     6421 gataatgtag ttttagttgg ctttggtgga ggtctaactt ggggggcttc agttgtcaag
     6481 tggactttat agtataaaat atctaaaatg atttaattaa caaaattaaa cttatacaca
     6541 aaataaagtt gattaataat aaaaagttta cttttatgat ttcaagtgaa ggagaattgt
     6601 atgaataaaa tttgcaaaat attaaatata aaatacccag ttatccaagg gggaatggca
     6661 tgggtagcta ctgcatcatt agcaagtgct gtatctaatg caggaggact tggcataata
     6721 gcagcaggaa atgcaccaaa agaagctata aagaaagaaa ttgttgagtg taaaaaatta
     6781 acagataagc cttttggagt aaatgtaatg cttatgtcgc catttgttga tgatataatt
     6841 gatttgatta tagaagaaaa agttcaagtt attactactg gtgctggaaa tcctgcaaag
     6901 tatatggata gattaaagga agctggaaca aaggttattc ctgtagtacc tacaatagct
     6961 ttggcacaaa gaatggaaaa gctaggagct acagcagtaa tagcagaagg tactgaaggt
     7021 ggaggacata taggagaact tactactatg gtcttagttc cacaagttgc tgatgctgta
     7081 aacatacctg taatagctgc tggaggaatt gtagatggta gaggaattgc agcatcattt
     7141 gcattaggtg ccagtgcagt tcaagtagga actagattta tttgcagtga agagtgttct
     7201 gtccattcaa actataaaaa cttagtacta aaagcaaaag atagagatgc aattgtaaca
     7261 ggaagaagta ctggtcatcc agtaagaaca ttaaaaaata aactatcaaa agaattttta
     7321 aagatggaac aaaatggagc tactcctgaa gaactggata aaaaaggtac aggagcttta
     7381 agatttgcaa cagtagatgg agacatagaa aaaggttcat ttatggcagg tcaaagtgct
     7441 gctatggtaa aagaaataac accttgtaag gaaattatag aggctatggt aaatcaagca
     7501 agagagatta tgccagcaat agaactgtaa atcaggagga gcaagatggg aaaagttgca
     7561 ctagtattcc ctggtcaagg agctcaatat gtaggtatgg ccaaggattt atatgaaaat
     7621 aatgctgttg ctaaaagtgt aatagatgag gctagtgatg ccttaaatat ggatttaaaa
     7681 aaccttatgt ttaatggaaa tgaagaagaa ctttctaaaa ctgaaaacac tcaacctgct
     7741 atggttacac atagtgtagc agtattaaaa gcggtacaag ctcaaataga tttaaaatat
     7801 aatgcatgct taggattatc cttaggagag tatagtgcgt tggttgcagc agatgcaatt
     7861 gactttaaag atgctgtatg tttagttaaa aaaagaggta agtttatgca ggaaacagtt
     7921 ccagcaggtg ttggagctat ggcagcagtt ttaggtttag atagaaatgt acttgaaaat
     7981 gtggttaaag atatacaagg aggtattgta gaagtagcaa attacaattc tcctggacaa
     8041 atagttatat ctggtgaaaa tgaaaagata gaagaagcaa tggtaaaatg caaagaagca
     8101 ggagcaaaaa gggcagttaa attaaatgta agtggacctt ttcactcatc aatgttaaaa
     8161 gaagctggtg taaaattggc cagtgaatta gaaaaagtta acataactaa acctaaagtt
     8221 gatgtggtgg caaatgtaaa tgcagattac tataaaaacg aaggtaaaga cctgcttata
     8281 aaacaagtaa gttcatctgt tctttgggaa gattctatag aaagactttt aaatgatgga
     8341 tatgatactt ttatagaaat gggacctgga aaaactttaa aagcatttat taagaaaata
     8401 gcttccaata aaaaagcaaa tgtcaatatt tataatatag atggtataga ttctcttaat
     8461 gaatttgtac aaaattatag aaatggagag atataatgat aaatcttaca ggacaagtag
     8521 cagttgttac tggtggttct aggggtatag gaaaagaaat agcaaaaaaa ctagcatctt
     8581 ttggagctga tgtagtaatc aattatactt ctaaagaaga tgaagcacta aaaactaaaa
     8641 atgaaataga aagtatgggg gtaaagtgta cctctataaa atgtgatgtg tctaaatttg
     8701 atgaagtgaa tcaaatgata gattctgttg taagcgaatt tggaaaaatt gatatattgg
     8761 ttaataatgc aggcataact aaagatggtc tgcttatgag aatgaaagaa gaagattttg
     8821 atagagttat agatataaac ttaaaaggtg tctttaattg tacaaaagca gttactaaac
     8881 ctatgatgaa aaagaagtat ggaagaataa taaatatgac ttcagtagtt ggaattatgg
     8941 gtaatgcagg gcaaactaat tattgtgcat caaaagcagg tgtaattgga tttacaaaag
     9001 cttctgcaag agagttagca tcaagaaaca taaatataaa tgcagtagca cctggattta
     9061 tagaaacaga tatgactaaa gtactaagtg atgatgtaaa agaatcaaca ctagcaaaca
     9121 taccaaagaa atcttatggt aaaccagaag atgtagccaa tgccgtagca tttttagtta
     9181 gtgacatgtc aagttatata acaggacaag taataaatgt agatggtgga atggtaatgc
     9241 aataataatt taaaaagtag agaaatctat taaaataaaa aattttagga ggtacacaat
     9301 gtttaataaa gttgtagaaa taataataga gcaattagga gtagaagata aagaaataac
     9361 tatggaaaca tcattaatga aggatttaga agcagattca ttagatgcag tagaaataat
     9421 aatggcactt gaagatgaat ttggtataga aataccagat acagaagcag aaaacttcaa
     9481 atctatagga gatatagtta agtatataga agctaataaa tagtcgagct ttaaaaaagt
     9541 ggaggtatcc gatgaataaa agagtagtaa taacaggact tggatgtgtt acgccgttgg
     9601 gaacaggaaa agaagagttc tggagtaata taaaatctgg agtatctggt atagataaaa
     9661 taactaattt tgatgctagc acatatcaaa ctcagatagc aggagaagtt aagaattttc
     9721 atccagaaga atatataagt aaaaaggaat tgaagagatt agataaattt gctcaatttg
     9781 caatagtatc tgctaaatta gcagttgaag atgcaaatct tgatttagat aaagttgata
     9841 gagaacgttt tggtgttata ataggttctg gaataggtgg agttgaagct atagaaactc
     9901 aacataaaat tcttttggaa aaaggaaata aaagagtaag ttcacttttt gtacctatga
     9961 tgataggaaa tatggcagct ggtcaagttt caatattttt aggagcaaaa ggacctaaca
    10021 ctaatgtatg tacagcttgt gcatcaggaa ctcattcaat aggtgatgct tttaaggtaa
    10081 ttcaaagagg tgatgcagac cttatggtag cagggggttc tgaagctgct gtaacaggtc
    10141 ttgcttttgc tggattctgt aacatgaagg ctatgtcaac tagaaatgat gaccctaaaa
    10201 ctgcatcaag accatttgat aaagacagag atggttttgt aatgggagaa ggagcaggta
    10261 ttgttatctt agaagactta gaacatgctt tagcaagagg tgctaaaata tatgcagaag
    10321 tagttggata tggtttaact gctgatgcat accatatgac aactccagct gaaaatggag
    10381 agggagcagc taggtctatg aatatggctt tgaaagatgg aaatgttcca ttagaagaag
    10441 tagattatat aaatgctcat ggaacttcaa cttattacaa tgatttatat gaaactatgg
    10501 ctataaaaac tgtatttgga gaaaaggcat atgacttatg tgtgtcatca acaaaatcta
    10561 tgactggtca cttattagga gcatcaggtg ctatagaggc tgttgtttgt gctatgagta
    10621 tagaagatag ttttgtgcct ccaactataa acatacaaga agttggtgaa gatttggatt
    10681 tagattatgt tccaaatcaa ggtaaagaaa aaaatattag atatgcacta tcaaattctt
    10741 taggatttgg tgggcataat gctacaatag tactaaaaaa atatgtataa ataaatatta
    10801 aatataaaac taaaaaacaa gcacttgttt taactgtata aatttatatt agcttaaatt
    10861 taaagtctcc taaattattt taggagattt ttttattaag atttaagtct aggatatgtt
    10921 gtaaatcatt ttgatatatt gtattattaa ttattaagag gttatttata tatgcaagtt
    10981 ttcattctac aatatacaaa aggaggacta aaattttgaa ttttagacta ccaaagaaaa
    11041 ggttacccaa aaagataatt tttatatgta taatacttat tagtattatt ttgtttataa
    11101 agtatgacca aaatggattg aaagaaagag aaattataaa taagggaaaa tatatgtctg
    11161 aaagccttgt ggcagataag gagtatgatg aagcaaaaaa agtggttaag acttcttttg
    11221 aatctattcc agaaaaaaaa tacaataaag tatataatga agtttgggat atgttaaaga
    11281 ctatttcata tgttccagat ggaacgaaga tagtcattga ttctttagaa aagatgagag
    11341 aatcagatgt gaaattatct gatgaatgta gatttaatat agaaaaaaga ttagcatcag
    11401 tgtatataat ggataataat tattctaagg cagtagattt aactgtaaag tctataaagt
    11461 tggctgagaa attggggaat aattatgaaa aagcaagact agatgttgat ttggcgagca
    11521 tatttttaaa gataggtggc tatgaaacag gagagaaatt aataaaggat tcttttaaaa
    11581 tagatattga tgaaggtgaa aaaaatggaa tggttagatt atatgctatt ataaatctag
    11641 cagaaatata tgtagaagag ggcgaatatg ataaagctat tgaaataagt agcaggataa
    11701 aagattataa aaagtttgta aatacggata attacaatga ttttgatata atggcctcta
    11761 taatgcagtc aaatgcgtat gtgggcaaaa ataatataca aaaggcaaag acttttttag
    11821 aaaaagctga ttatcttata aaatcagata gaacagttta tatattagat aaagatatgt
    11881 actattatat ggcaatggga aacttagaat ataaaagcga aaattataat tctgcaatag
    11941 ataattataa aaaaagttta gagatatcta ctaaaagaaa gttaacccag gagaaaataa
    12001 aaaatttaaa taagatatta aatatatatg aaaaacaagg aaatatagaa tctttaaatg
    12061 agtatcaaaa gatgttaata aatgaatata aagagaacaa aagtataaga gattcagaga
    12121 ctagtttcta tataattgat aaagtatcaa acgaaagtaa gctatttgaa aaaactaaaa
    12181 aagaaataaa atattataaa atattattta cagtaatttt aattgctata ttagggtcta
    12241 cttttttata caaccgattg aagtatttta aagcacaaaa tttacatgat ggtcttacaa
    12301 atgtttataa tagaaaaagt tttgatatta tgtatgaaaa gtacagagag aaagatgata
    12361 attttgcact tgtgatgata gatattgata atttcaagct cataaatgat aattatggac
    12421 ataaatttgg tgacactgtt ataaagggaa taacaaatac catttgtgtt atgttagaac
    12481 aagatgataa agtctttagg tacggaggag aagaattttc tgtattaata agaaataaat
    12541 ctggtgatga agtagcagat ataattgaca gtataagggt tgcaattgca aataaaaagt
    12601 ggaatgatga tgtgactgtg actataagtg caggtgtagc tcatgtttca gatagcaaag
    12661 atgtactaga agaagctgat aaaaatttat ataaagcaaa acaacttggt agaaataaag
    12721 tagtatataa gtaattatat actatataaa taagaggtat aaatatgaaa caatcaatag
    12781 ctatagtaac tgataaaata actaaactat catctttctt agaagaaaat atacgtttgg
    12841 ttttaggtaa ttatattgat ataaaccact attatctgga agttttaaaa gagaatgata
    12901 aaatacaagg tgattttgta ctagtaatga atgatgatag attaaacagt atgagaaagc
    12961 atttagtaca agatagtaag attatagtga taaggagaac acttaaagaa aatgaaatat
    13021 atggattatt ttcaatacca aatggaactg aagtattggt tgtaaatgat acaaaagaaa
    13081 ctaccttaga aacgataagc ttattttaca aaataggagt aaaaaattta aagtttactc
    13141 catatattga aggaaaaatt tataaaaata tagacatagc tataacacct ggagtaaagg
    13201 gaaaagtacc aagttatata aataaagtaa tcgaccttgg aaatagatat attgatatat
    13261 ctacctttat agaaataata aatggtctta gaatagatgt taaagaaata cgtaaaaatt
    13321 taatggcata ttctgagagt ttaataagtt tagataatgg agttaaagat aattataggc
    13381 aactgttttt aaaaatagaa gaacaagatg ttatattgaa tctatcaaaa gatggaataa
    13441 tatttacttc agcagatggt ataataaaca catttaataa agaagcttta aaaattttag
    13501 gtataaatga aaatccacaa ggcaaaaaca tagaagatat catttctgaa gatttaaata
    13561 ttttacttga agacaaagaa ataattgatg aagtggtatt tacaaataaa aaatatataa
    13621 atgtaaataa aaaaagtatt ttttctatgg ggaataaagc tgggatatat tataacttac
    13681 aagaaataac gtatataaaa aaattagaac aaaatcttac taataaatta agagaacaag
    13741 gtcagatagc aagatataca tttaaagata taaaaactaa aaataaaaat atggtaaagt
    13801 gtgtagaatt ggctaaaaaa atatctaaat cagacctttc tgtattaata ataggagaaa
    13861 gtggtactgg taaagaatta ttatcacaat caattcataa tgcttccaat agaagtaatc
    13921 aaccgtttat agcagttaat tgtgcagcag ttccagatag tctattagaa agtcaactgt
    13981 ttggatatga aaaaggctct tttacaggag ctttaagaga aggaaaaaag ggtctatttg
    14041 aacttgctaa taatggaact atatttctgg atgaaattgg agatatgcct ccattgctac
    14101 agacaaagtt actaagggta ttacaagaaa agcaggttat gcctataggt tcccataatg
    14161 ttataaacat agatgttagg gtcatagcag caacaaacaa aaacttacta aagatgatta
    14221 gagaaggaaa atttagagag gacctgtatt atagactaaa tgtattgcca atagatgtac
    14281 catcattgag gaatagaaaa gaagatataa taacacttat gaatttcttt ttgaaaaaca
    14341 atataagact ttcgccagaa gttataacta ttttagaaaa atataattgg ccaggaaata
    14401 taagagagtt acaaaatgtt gcttcttatg taagtctaat gtgtgatagt atggttttta
    14461 gagatgactt accaccatat ataagggtag aatgtgctaa caattatgaa gatggatttg
    14521 gttatacaag aaaagaattt gaggaaatac tgaggattct atatgaaaga agagagtcat
    14581 cctctggaat tggaagaggg tttataatcg aaaagcttat acaggaaggt attgagattt
    14641 ctgaaagcaa agttagaaag gtgctatctt atttaagtga atgtggatac atatcatcta
    14701 aatctggtag aggaggaagt caaataacct cagaggggat aaaactttat aataatataa
    14761 attaagctaa gtatttgata ttgaaatcac atattttgaa tttagatatt taaataataa
    14821 taaactttta tatttataga gattaattta tattatgtca gaaagaatat tgtaatttat
    14881 aattaaatat taataggttt ttaaaatagg attagatagg taataggtta agttagaata
    14941 acccattacc tatttttatt tgtaagaaaa gtgtacatag aattagaagt aaaaaataca
    15001 actaataaaa ctattgaaaa tactagataa ataataaata aaaataatta tagattttgg
    15061 catacatatt gctaattata gagataagac gacaaaataa ttgggaggtt taatgatgaa
    15121 atacaaaaca acagggactt gtgctacaga aattgaattt gaagtaaaag aaaataaagt
    15181 tacaaatgta aactttatag gtggatgtga tggaaattta aaagggctaa aagtattagt
    15241 tgaaggcatg aatatagaag atgtaataca aaagttaaaa ggaatagagt gtaaaacaaa
    15301 gccaacatct tgcccagacc aactgtctct agcattggaa aattatatga atataaagta
    15361 atacataaaa caatcgatta gttaggagtt taattatgaa accaataata gggattttag
    15421 gaaatttaat aataatggaa aatggtatgt ttccaggtct tgaaaggtca tatgtaaata
    15481 atgattatat aaatgcagtg ctaaaaggtg gcggtagtcc agtaataatt cctgtaaata
    15541 cagataaaga agttataaaa aaacagattg aaatggttga tggagtctta atatctgggg
    15601 gatgggatat caatcctcaa ctgtatggag aagaaacaag agaagaaacg acatttatat
    15661 atccagaagt ggatgaattt gatttaattg caataagtat agcattagaa cttaaaaagc
    15721 ctatattagg tgtatgtagg ggactacaaa ttttaaatgt aagtttagga ggaactttat
    15781 atcaagataa taatcttata gaaggaagtt atataaaaca tacacaatct tctaaaagac
    15841 atgtagctac tcataaagtt gatgttaaag aaggaagtat tttagaggga attttaggaa
    15901 aacaattact tacaaatagt tatcatcatc aaagtgtcaa tcagcttgga aaaggattaa
    15961 aagccatagc atactcaaaa gatggtataa tagaagctat tgagaaagag gatgaaaatt
    16021 ttgtagtagg agtacagtgg catccagaaa tgatggtaga ttactgtgat aaaatggaaa
    16081 aactatttaa atattttata aatatttgtt cgaatttata atataccttg gggggattga
    16141 acatgaacga aaagaacaaa atgggattaa taagtattat acttttaggt attaatgcag
    16201 tagtaggagc tggagttttt ctattaccag gagatgcaat gaaatcattt ggtgtagcaa
    16261 gtatatttgt atatatattt gatatgttgt tagttttatc tatggcattc tgttttgctg
    16321 aggttgcagg taaattcaat aaaaatggtg ctgcctatgt atatacaaaa gaagcttttg
    16381 gagatttttg tggttttgaa gtaggtctta tgaaatgggt aataggatgt atttcttggg
    16441 gagctttaat agtaggtttt cctacatcat tatcagcagt atgggctcca gcaggtgaac
    16501 cacatataca aaaaattata attgtagcta tgatagttgg gcttacaatt ataaacttat
    16561 taggtgtatc tctatcaaaa attgtacaaa atgttataac tgtaggtaaa ctaattccat
    16621 taatactatt tataggaata ggtatatttt ttataaaagg agttaatttt acttcttcaa
    16681 caatggtacc tccaggagct ggagcaacag agtttggagc agcagcactt ttaatgtttt
    16741 attcatttac aggatttgaa tctatagcag ttgcagcaga agatatggaa aatccacaaa
    16801 aaaatatacc aatagcaatt ataagtgtaa tagtaattgc atcaataata tatatattaa
    16861 atcaagttgt atgtgttggt atactaggag attcgttatc tagtacatct acaccagtgg
    16921 cagatgctgc aagaatatgc tttggaaata tgggagctgg attggttacc tttggaacat
    16981 tggtttctgt tggtggaatt tgtatgtgtg gtgcttttgt aaatccaaga agttgtgtag
    17041 ctttagctga tgataaaatg ttaccgagaa tatttgcaag aaaagataaa aaaggtacac
    17101 catatgtagc gataatagct actatgttaa tcactatacc aatagctcta tctggaagtt
    17161 ttgctgaatt agcagcaata agtgcagttg ctagattcat acaatacata ccaacatctt
    17221 tatccgtatt agtgtttaga aagaaaagac cagaattagt aggtactttt aaaactcctt
    17281 ttggagcagt gattcctcta attgctgtat gtgttggaac atggttgtta ttccaagcat
    17341 ctatgcatca attaattatg ggattaggag ctttggcaat tggagtacca ttatacttta
    17401 taatgaagtc ttataataga aaaatctatg gtgaaaattt aaagaaaata gtttaattat
    17461 gttataacaa tttattgagt taaaattaga cattggcaga aaattaaata caaataagta
    17521 tctacaaggc tatattaaaa tattattaat tttttatttt aatatagcct tatattgtta
    17581 tcaataaatg aaaaatattg taacaacaga agaacaatag tattatgttt atagacaaaa
    17641 aacttattag gaggtaaatt tatgccaaca ataacattaa aaaatggtgt agacgtactt
    17701 attagagaag gtgtaagaga agatgctcaa agcattatag atttttataa tgaagtgggt
    17761 ggagaaactc attttctgtc atttggaaag gatgaatata aaatatcttt ggaggaacaa
    17821 gaaaatgcta tagaatcagc aaaagcatct gataattctg ttaaactgat tgcatttata
    17881 gatggtgaaa tagttgggat tgcaacgatt gattctaatc aaaaagcaaa aggaaagcat
    17941 gttggagtat taggaattgt tgtaaaagaa aaatattggg gaattggatt gggtaaaagg
    18001 cttatgttag accttataga atggtgtaaa tcaaatggaa taaccaagaa gataactctt
    18061 gtcactaatg aagaaaatta taatgctata ggattataca aaaaagttgg atttgaagta
    18121 gaaagcatac taaagaaaga atgttattat aatggtgttt atactgattt aataggaatg
    18181 tcacttttat tgggaatata attagtgtac attttatctc gaaaaaagta tattaagtat
    18241 ggtataatta aaaaagaata taacttaata taattttgga ggacgaatta tgaaaaatct
    18301 gtgtaaaata tcagatgatt atttaaacag caagcttaag tatttaaagt tattatcaaa
    18361 acaatatcca agtatatcaa aagcaagtac agaaataata aatttagagg ctatattaaa
    18421 tcttccaaag ggaacagagc attttataac agatgtacat ggtgaatatg aaccatttgt
    18481 acatgtatta aaaaatggtt ctggtgtaat aaagagaaaa atagaagaat tgttttcaaa
    18541 tacaataaga gacagcgaaa aaaaaatgtt agcaactctt gtatattatc ctgaacaaaa
    18601 gttagaccta ataattaagc aagaggaaaa tatagatgat ttttatagaa taaatattta
    18661 tagacttata gaactttgta aatatgcttc tagcaaatat actaggtcta aagttagaaa
    18721 actgttacca gaaaatttta aatacataat agaagaactt ttgcatgagc atgttaaaag
    18781 tgaacataaa gaagagtatt ataaaagtat agttgaaaca atagttgaca taggaatagc
    18841 taaggagttt ataattgcta tttctacagt tatacaaaaa ttggttgtag atagacttca
    18901 tgtaatagga gatatttatg atagaggtcc aagaccagat ataattgtcg ataagcttat
    18961 tgaacatcat tgtgtagata ttcaatgggg aaatcatgat atattgtgga tgggtgcagc
    19021 ctcaggtgaa aaaacttgta tagcaaatgc actgaggata tcagctagat atgcgaattt
    19081 agacattgta gaagacatat atggaataaa tctattgcct ttagccactt ttgctataga
    19141 aatgtataaa gatgaccctt gtaaagaatt tattccaaag gttaatgacc aaagtgtaac
    19201 aacaacagaa aagtctttaa tggcaaaaat gcacaaagca ataagtataa ttcaatttaa
    19261 gcttgaaggt gaggttatca gacgaagacc tgaatttgaa atggaacata gactcttgct
    19321 taatatgata aactatgatg aaggtacgat taatttaaaa ggtaaaacat ataaattaaa
    19381 agatacttat ttacctacaa tagacaaaaa agacccatat aaattaacta tggaagaaag
    19441 aaatgttatt gataaattag tatcttcatt tagaggtagt gaaaaactac aaaaacatgt
    19501 ttcatttttg ttttctaaag gaagtatata tttaaaggct aactcaaatt tactgattca
    19561 tggatgtgta ccgttaaatg aagatggaag ctttatgtct atgaatataa tgggaaaaga
    19621 gtataaaggt aaggctctta tggataagat ggagtctcta gcaagagaag gatttttctt
    19681 taaagataag gcagaagaaa aactgtatgg catggatatt atgtggtatt tatggactgg
    19741 gaaatgttca tcattatttg gtaaagatga tatgactaca tttgaaagat actttatagc
    19801 tgaaaaagag actcataaag aaaataagaa cccatatttt aaacttagag aaaatgagat
    19861 ggcttgtaaa agattatttg aagaatttga tttagaactt gatgaatctc atataataaa
    19921 tggtcatgtt cctgtggaga gtaaaaatgg agagagccca ataaaagcta atggtaaaat
    19981 tctcgttata gatggtggtt tttctagggc ttaccaaaaa actactggta tagctggata
    20041 cactttgata tacaattcta gaactttgca attagtatcg catgaaccat ttaattctgc
    20101 tgaagaagcc attgcaaatg aaagtgatat cttatcgaca actgttgtag tagagcataa
    20161 ggcaaagaga aaaatggtga gagatactga tgaaggaata aagatacaag aggaaataga
    20221 agatttaaaa ctacttttaa tggcttataa gaaagggcta ataaaagaaa tgtagatgtt
    20281 atagaggcac ttcatgattg aagtgtcttt ttgttaatac ttattaaata tattataaag
    20341 attaataata gtagtttata ttatacttac taagtgaatc caagataaat gggttcactt
    20401 tttttattta taaacattat taaaaaaata tagatgtttt ggttatattc cttgtccttt
    20461 ataaatagaa ttatataaac aaaggaggag gacaagagat gaataaactt tctgatgaaa
    20521 taataaactc tctttctaca actataagag aaaaggtaga aaaagtctca aacaataatc
    20581 tgaacataga agaaattagg ttacgctcac aaaaaccatt gatactaaat gcaaactcta
    20641 aagattattt ttacaatcaa aaaactatga cacttgattt aaatcaacaa aactcatatg
    20701 tagtaacaag agaagatgta gagcaaactt ttcaaataat atgtaaatat tcaatacatt
    20761 catttatgga tgacataaaa aaaggattca taactttgag aggaggtcat agagtagggt
    20821 tagttggaaa agcaatagta gaagatggac aagtcaaaaa tataaagcat atatcatctt
    20881 taaatataag ggtatcaagg gaaattattg gatgctcgga taagatttta agtcatatca
    20941 taaagggaaa aaatcagatt aataatactt tgataatatc accacctcaa tgtggaaaaa
    21001 caactttaat aagagatata gtaagaaatt taagtaatgg taatgaagat tatggattta
    21061 aaggtttaaa agtggcttta gtagatgagc gtaatgagat agcaggagct tatctaggtg
    21121 ttccacaaat ggatgtggga ataaggactg atataataga aacttgccca aaagacttgg
    21181 gtattacaat gcttttaagg tctatgtcac caaatgttat tgtgacagat gaaattggaa
    21241 gtgaaaaaga aataaaggca ttatacacag cattaaatgg aggtatagga ttaataacta
    21301 ctgtgcatgg agattcaata gaggatatac aaaatagaaa agaattaaat agacttctgg
    21361 ataaagagtt atttaaaaag gttataattc tttctgcaaa aagaggagca ggaacaattg
    21421 aaaaaattta tgatttagaa gaaaagagat ggtattttgc aaattaaaat aattattata
    21481 gctttcttga taggaagtag ttatttgata ggtgaacaaa tatacaaaac ttatactcgt
    21541 agacataaac aattaaacga tttaataaga gttttagaaa ttttgaggat ggatttatct
    21601 tttggacttt atacattaga agaaatcttt aataggatag gtggaaataa agagttttgt
    21661 ttttggaaat ttttctatca aatatcagaa ggacttcaca atgaacaaag taaaactttg
    21721 gaaataataa tatcagaaaa tattgatgtc ttaagtaaag aaacttatct aggcaataaa
    21781 gaaattgagg aactgaaaaa tctaatactt actttaggga aaagcgatat agagtcacaa
    21841 caaagaatga tagatttatc tatagaaaat ttgaaaaaac aaacctatga gacaaaggaa
    21901 gatataaaca aaaaaggcgt attgtataaa aaattagtga cattcatagg tataggtata
    21961 tgtattatat tgatttaata tggggaggtt tattatggaa atatcattga tattaaaggt
    22021 tgcaggtgtc ggtattctaa tatcagtatt gaatatgata ttggaaaaaa cagatagaaa
    22081 agattgggca ggtcttacta cattagcagg tgtaataatc gtattgggta tggtaataac
    22141 agaaataagt gacttgttta atactgtaag aacaatgttt caactttact aaagaggagg
    22201 gagtaatttg gaaataatgc aattaatagg aattgcaatc atttcaacta cactctgttt
    22261 agtaattaaa aaagatagac cagagatagc aaatttcata gctataataa ctggagtaat
    22321 aatactattg tcagttatgt ttaagctaaa ttttatagta gatagtatac aagatttggc
    22381 taacaaggct aacatcccaa ctatgtatat atcattaata ataaaattaa ttgggatagc
    22441 atacttaatg gaatttgcga tacaactttg taaagattgt ggggaaggaa acattgcttc
    22501 aaaactagaa tttggaggta aaataatagt tatgtcaatg tctttcccta tacttttgtc
    22561 cattgtagaa atggtggtga atataattcc atagatgtgt atcttggaat gaatataagt
    22621 atattagaga gctttcgttg tattgaggtt tatagagtga agagaggcat ggttgcttat
    22681 gaaaaaaaga tttttaccaa tgattatagg ttttttattt actttctttt ttgtaaatat
    22741 ctttgctatt atgatatttg ctaatgaagt accaagtagt gaagataaag aatatggtga
    22801 aacaaaaaat agcatagata agtatataga tggtcaatta gacaagctag atataaatga
    22861 gattcaagac tatataaata aagaaatagt tattaatgat gttaatttaa aatcatttgt
    22921 aaaagattta attagtggag aaaaaaatat cttagattta ttcaataaag atggattgaa
    22981 gatattgatg tttgatgaat ttaaagcaag tttaaaggta gtagcagtaa ttttggtact
    23041 ggctctgtta tcatctattt taaagagtct ggaaaactct ttttcatcag gtgcagtaag
    23101 tcaaatagca acatatataa tatttatcac aatggtatca cttacactta ttggttttaa
    23161 agatgtatta cagatttgct atgatgcaat agaccatact gtaggtctta tgcaagttat
    23221 aatgccaata ttaataacat ttttactttt aattggattc ccaataacgt cgactacttt
    23281 gaatcctatc ttcataggag gagtaacatt tataaatgta ttttttaaaa attttctatt
    23341 tgtatctatt actgttgcat ttggaatttt aataataaac aatttatcaa aaaatataag
    23401 gctaaagaga tttttctcct ttgtaaaaca aataaattat gtttctattg gagctatgtt
    23461 tactgtttac ttaggtcttg tatccataca aggattgtat gtaaccagtt ttgataaatt
    23521 cagtgtaaag actgctaaat ttgctatagg taattttatt cctgttgtag gtggatttgt
    23581 atctgactct gtggatattt tattatcttc atctcagctt ataaaaaata tatttggagg
    23641 aataggactt atcttacttg ttggaatttg cttacttcca gtaataaaaa ttttatcagt
    23701 aatagtagtg tacaaattag cagcaataat tgtagagcct gttggagagg atggaatatc
    23761 taatttttta aatgaagtgg caaatttaat gataataatg ctagcatcag taattgctat
    23821 aacagtaatg ttctttgtaa ctgtagctat cttgacctcg ataagtgtcg taagtcaagg
    23881 gtaatttata tatcagtgaa aaataattac atttaatcta atgtttttag agaggaggat
    23941 gctatgttag agggcataaa ggcatggata gttagtgttt tgataggagc atttatagta
    24001 aatatagtag atatgatttt accaagttct aagataaaac cttatgtcaa cctagtttta
    24061 aactttatgt ttgtatttat tgtaataact ccagtagtag gatttttttc aaaagatatg
    24121 agtttagaag atagaatatt aaaatctatg ggaaactaca acaagcagta tgtagatagt
    24181 acaaatgctt tggcaaaaga gactggaaat aatagtctat ccaagggata tgaggatggg
    24241 ttaaaagaag tacttaaatt aaaacttgat gaatatggtt atgatttaga agatatagag
    24301 cttaatgggg caaatataaa taatataaaa ataaaagaaa agaataatag tgcaaaaagt
    24361 aataacagtt ccaatgaaga aaataaaaat aatagtacta aaagagagga agaaaacata
    24421 aactctaatg acaaagaaaa ttctaaacaa gtcttcaaaa aaggtacaga gtatgggttg
    24481 aacctaaatg aagaaaaatt aaaaaacgac ttaataaagg tgctggatgt ttctatagaa
    24541 gatatacaaa ttgataaata ggagttggga gaatgttgaa aaacttaaat gacaaagata
    24601 agagaaaggt atattcacta ataactatag caggaatatg tgtggtttca ttagtattat
    24661 tatcttgttt tcctagtagc aaaagcgata aacaagttgg aaaaacagag actaataaaa
    24721 atgctgaaaa gcaagtcact aaagaacaag aaaaagatga tttagagtca aaattaacag
    24781 caatactatc taaaattgat ggtgcaggag acgttgatgt aatggttaca tttgaatcaa
    24841 gtgaagaaat acaacctgct tttaattcaa ataatacaac agaaacaaca gaggaaaaag
    24901 atgcacaagg tggagaaaga acagttacga cttctagtga aaataaaaca atgataactt
    24961 caaattcaag tgaccctgta gttataaaaa ctacagagcc aaagataaag ggagtgatag
    25021 tggtagcaag tggagcaagt gacccaagtg ttaaagagac actttatagt gcagtacaaa
    25081 catcattaca agtagctggt catcaagtag aaatatattc taaataaata catataaccg
    25141 gggggataaa tgaatgaagt ttaattataa gggaagagga tttgtaataa taactttaac
    25201 tgcaatgtta gtggtagtag gaacagtgaa ttatcaacta agtaagaaat ctttattaga
    25261 aacatcaaaa gagtttaagg catatgaaga agcacagttg caaaaaaata ctgatgagag
    25321 tgatagtagt aagacagagg attctagtaa tgtagataaa caagatggta aggaaagtgc
    25381 tgatattgat atagttgata gcaaagctag taaagtaaaa gaaaaggcaa ctgagacaag
    25441 taaggaaata aaagctcaat tatcatcaga gaaaaatatg aaaaaggcat cttatatttt
    25501 agatatgaaa atgaatagag aaaaacaaag aaatgagtta gttcaagatt taaatgaaat
    25561 gataaataat ccatctacaa cagaagaatc tagaaaagaa gcttctaata tgaaattaaa
    25621 tatagttaaa attcaagaaa aagaacttca aatagaaaac cttttgagta caaaagggta
    25681 tgaagaagcg ttagtttata taagcgataa caaagttaat gtagttgtaa atgaagctaa
    25741 attagaaaag aaagatgcag caaaaatatt tgatttagtt gcagaacaag ccaatgtgaa
    25801 gtatgaaaat attaaactta caaataataa tagtaataag taaagtataa acctcagaat
    25861 taactgaggt tattttcttg caaagtagac ttgtatttgg tattattata cttataaaaa
    25921 tagttcctta aaatcaggag gtaagtttta atatggaaga taataaattt ggacaagtaa
    25981 aaatatctaa tgatgtaata gctacaatag ctggtttagc agcattagaa gttgaaggca
    26041 tagaaactac agcaacatta acagataagt tgttaaaaaa taatggagta aaaatacaaa
    26101 tcgaagaaga ggatgttaat ttagatgtaa tggttacgat aaagtatgga atgtcaatac
    26161 cagatacagc ttttaaagtt caagaaaatg taaaaaatac tgttgagaca atgacaggac
    26221 tgaaggtttc tcaagtaaat atacacattc aaggaatcag ctttaagaag gataaagttg
    26281 ataaagaaga agcaaaagca actaagaaaa attaatatta gccctctgaa aagtcagagg
    26341 gttttttgct ggtaattgat tttatatgga taattaaact taaaggagaa ttttgattga
    26401 tgaaaaaaga tagggcacaa aagagtacaa caagagaata tataatgaaa cttatatacc
    26461 aaataaatat taataaagaa gattttgaaa ctttagaaga taaagtagat aattttttaa
    26521 aagataattc agaacatatt ataaatagat ataaagaact tgcacttcaa tactcaaaaa
    26581 atacaaattt aaagttagaa gatactgaaa ttgaagatgt tatagacaaa aaatatataa
    26641 atacagtatg caaagcattg aaagaaaatc atgacaaaat agatgagtta ataaataagc
    26701 atgctaaaaa ctggacagta gatagaatgc caaaggtaga tgtttctata cttagattgt
    26761 ctgtgtgtga aatactttat ttagacactc ctaataaagt atcaataaat gaagcagtag
    26821 aacttgcaaa aatatattgt gatgataagt ctcctaaatt tataaatggt atattgggaa
    26881 gtgttgttga tgaaattggg aaataatata ataattggaa ttgatactag ctgctatact
    26941 acttctatag cagctatctc tttagataag aaagttatat tcaatgaaaa aataatgtta
    27001 gaagtaaggg ataattcaaa aggtttaagg caaagtgaag ctgtatttca acatatcaat
    27061 aatttaggga ttcttagtga taggataaaa tcttttaaag ataagtttaa tgtagaagga
    27121 gtttgctcat caaaaaaacc gagacctgtt gaaaactcat atatgcctgt attcaatgta
    27181 ggtcataatt ttggaaaatt attgtctagt atatatggtt gtaggtttta tgagactact
    27241 catcaagaga atcatattga agcaagttta ttaaatagta aattaaaaaa taataataaa
    27301 tttatttctg ttcatatgtc tggtggtact acagagatac ttttaacaag caaacaagat
    27361 agtcatcaca atgtatgtga tactaattta ggtaaaattg ccaaaataag tattaaaaaa
    27421 gatgataaaa gtaagttata caataatttt ggatataata tagatataat tggaggaagt
    27481 aaagatatta gctttggtca acttatagat agagtaggaa ttaagcttgg atataaattt
    27541 ccttcaggaa agtatttgga tgaaaatgct ttaaattgta atcttaaaat agaaagtggt
    27601 ctgaaaactt ctgtaagaga tgggtatatg aatttatcag gattagaaaa tcaggtaaat
    27661 aagattataa atgataatgg agataatact aatcaaaaag aatatatatc taaattggta
    27721 ttagattcag tagttagaaa tatgtttaaa tctttagtgt atttatgtga aacttataat
    27781 gtaaatgaag tagtatttgc tggtggagtt tctgcaagta agtatattct tagagaatta
    27841 agcatgaagt taagaaaaaa gcatatagaa gcttatttta cagaaccaca atattcaact
    27901 gataatgctg tcgggtgtgc tataatagga ttgaataact ttttaggaga aagagtatga
    27961 aactaagagc tttagatata agtgaagcaa actcatatat taaaagaatt ttaataaacg
    28021 acccaatact ttctaatctt aaagttaaag gagaaatttc taattttaag gttcatagta
    28081 gtgggaatgt atatttatca cttaaagatg aaacatcaaa gttaaactgt gtaattttta
    28141 aaagtaattt taatcgcaat ttaaaattag ataatggtgt aaaaattata gctaatggat
    28201 atatatcagt ctatgaaaga gatggtgcgt atcaattata cattaatgaa attgaaatag
    28261 agggaatagg aaatttacac atagagttta atagattaaa ggaaaaactt aataaagaag
    28321 gattatttga ccctaaatac aagataccta tacctaaaat gccaaactca attggcgtta
    28381 taacatctcc aacaggtgct gttataagag atataataaa tgtaataaaa agaagatatc
    28441 cgaaagttaa tataaagctt tatccagtaa cggttcaagg agataaatca gctgaagaaa
    28501 tctgtgaagc aattagattt ttcaatcata tgaagaatgt tgatacactt atagttggta
    28561 gaggtggggg ttcgatagaa gaactttggt cttttaatga tgaaatggta gcaagagagg
    28621 tttttaattc tcaaattccg ataatatcag cagttgggca tgaaacggat tttactattt
    28681 gtgactttgt gtctgatatg agagccccaa cgccatcagc agcagctgag attgctactc
    28741 catcattgga tgatataaac tataaacttg gaaacataaa aagtaggatg agtaagtctc
    28801 ttacaaatca aatagagcta gaccaatata ggttagaaac agtatttaat aagataaata
    28861 actatttaga ttcgtacaca ataaaagata aagttataca attagacaaa atatatgata
    28921 aaataatttt tggaatagaa aataatttaa aattagaaga tgaaaaatta gtaaaaattg
    28981 gagcattact ccataattta agtccattag ctactatgga tagaggttat agtataactc
    29041 aaaaaaatgg aaaagtaata aatagtataa aaggattaaa aataaaggat agtatagata
    29101 tagtattaaa agatggaaat ttagaatgta tgatagataa aattgaaaat aaagagggat
    29161 agtatgaatt taacctacga agaagcttat aaaagattag aaagtatttt aagtgaatta
    29221 gaatctaaaa atgctagctt ggatgaatct ttaagcctat atgaagaagg tataagcctt
    29281 tataaacatt gtaataagct tttagatgat gctaaattga aaataagtaa atttaatcaa
    29341 ttgggaatag aagaagactt taaaatagag gaggaataaa attggaattt aaacaatgtc
    29401 taaaagaaaa agcaagtttt gtagaaaaag tacttaaaga atatatgcct aaagaagaag
    29461 ggtatcaaaa aactgttatt gaagctatga actatagctt aagtgctgga ggtaaaagat
    29521 taagaccaat acttacattg gaggcttgta aaatagttgg aggtaatgaa gatgaagcta
    29581 ttccttttgc tattgcaatt gaaatgatac atacatattc actaatacat gatgatttgc
    29641 cagctcttga caatgatgat ttaagaaggg gaagaccaac aaatcataaa gtatatggag
    29701 aagctatggg gattttagca ggagatgctt tgttaaacta tgcttttgaa gtaatgcttg
    29761 caggctcaat caataaagaa aatccagaaa agtatctaaa agctataaat gaaattgcca
    29821 aaggtgctgg tatatatgga atgataggtg gtcaagtagt agatgtagaa agcgaaaata
    29881 aacaaattgg aaaagaaaaa ttagattata tacatatgaa taaaactgct gcaatgatgg
    29941 taggctgtat gagagcagga gctactatag gtggagcaaa ttctgaacaa atggaagaaa
    30001 taactaaata tgcaaaaaat atcggattat cattccagat agtagatgat atactagaca
    30061 tagtaggaga tgaagctaag ttaggtaaaa aggttggaag tgatatagaa aatcataagt
    30121 caacataccc atctctttta ggtttagata aatctaaaga aattgcacat aatttgattg
    30181 atgaagcaaa gaaaagtata gaaaaattat ctgatgatgt agatttttta aagggattgg
    30241 ctgaatatat aatagataga gaatactaaa atatacagct aaaatcgagg agggaaaaat
    30301 ggattttttt tcggagattt ttaacaatgg ggctttggga ataagtctga ttgcttgttt
    30361 cttagcacaa tttataaaaa tatttactgg taaggaaaaa agaattgagt tatcaagaat
    30421 acttatttca ggaggtatgc ctagttccca tagttctttt gttacaagtt tagctacagt
    30481 ggtaggtatt gaaaaagggt ttaattctac tgattttgct ataattaccg tgcttgcctt
    30541 aattataatg tatgatgcag caggtgttag acgggctgtt ggtaaacaag ctactatatt
    30601 aaatcaaatg gtggcagata tacagcatgg aaagcatata gagcagaaga aattaaaaga
    30661 attaataggt catacaccac tagaagtttg gtttggagct ttacttggaa tagtaacagc
    30721 tttaatattg atgtaaattg aaaatttaca gaataaaggt gaggataata tgtataaata
    30781 tttagataaa gtaaattctc caaaggatat aaaaaatatg agtatagaag aaatggattt
    30841 acttgcaaag gatataagaa agttcttagt aaaatctgtt tcaaagacag gaggacactt
    30901 agcttctaat ctaggagtgg ttgagttaac attagcactt cataaagtat ttgatagccc
    30961 taaagataag attgtatggg atgtagggca tcaatcttat gttcataaaa tagttactgg
    31021 aagaaaagat tgttttgtat ctttgagaca gtttaatgga ctaagtggtt ttccaaagga
    31081 gaatgaaagt cctcatgata tttttgatac aggacatagc agtacatcta tttctatagc
    31141 tacaggtatt gcctgtgcta gggatataaa aaaagaaaac tatagtgtta tatctgtgat
    31201 aggagatggt tctataacag gaggtatggc acttgaagct ttaaatcaat taggatatat
    31261 agataccaat atgatagtaa tccttaatga taatgagatg tccatagata aaaatgttgg
    31321 aggaatgtct aaatatctgt caagtattat aagaaactca acagttgaga aaatgacaga
    31381 tgaagttgat aaaattttaa atgttacaca aacaggtgaa attttatcta agactgcaca
    31441 tagatttaaa gataaactaa tgtatagctt ttctcctcag gactgttcat tttttgattc
    31501 attaggtata agatattatg gacctataga tggccataat actaaagaac taatagatat
    31561 attgagaaaa gctaaacaca aaaaagggcc tgttctttta catgtaatta cgaaaaaagg
    31621 aaaagggtac agatttgcag aagaacaacc tgataaatat catggagtat caaagtttga
    31681 tataaagaca ggtgttacat ctgcaaaagt taagtctatg tcaattagtg ttggagaaaa
    31741 acttgttgat atggctaata gtaatgaaaa tatagtagca ataacagctg caatgccatc
    31801 tggtacaggg ttaaacctgt ttgaaagtgc atatccaaaa agatattatg atgttggtat
    31861 agctgaacaa catgcaacag gatttgcagc aggtcttgca aaaaatggta tgaaacctta
    31921 ctttgctgtg tactcatcat ttttgcaaag ggcatatgac caagttattc atgatgtatg
    31981 cataacaaaa aagccagtta cttttcttat agatagagct ggattagttg gaaatgatgg
    32041 agaaactcat catggtatgt ttgatttaag ttacttaaat tctattccaa atattgtagt
    32101 aatggctcca aaagacacaa gagagatgga gcttatgatg gatttgtcat taaaattaga
    32161 ttgtccacta gcaattagat acccaagagg aagtagttat tacctagata agggagagta
    32221 tggagagatt gtactgggta aatatgaggt attagatgac ggtcaagata cagtcatact
    32281 ttgtattgga agtatggtaa aacatgcttt agaggctaaa gaaatattat caagagaagg
    32341 aataaatccg acaatagtta atgctagatt cttaaagcca attgatgaag gtatgttaaa
    32401 agcattatta aaaaatcata aaaatgtagt tactatagag gataatattg taactggagg
    32461 atttggaagt agaataaata agtttattat agataatgaa tataacgtaa atatacttaa
    32521 tatagcaata ccagaagaat ttgtaaaaca tggcaatata gatgaattat atgattttgt
    32581 aggattatcg ccaaaaagta ttgctgataa aataaggaaa ttagttattg agtaaaaatc
    32641 taatgtagat attgtaaatt aaaatttata taaaatatag aatttatcaa ttagaataaa
    32701 tatcaagtgt gttacttcaa gctactttag ttatcattaa agtagctttt aacagaaagg
    32761 taattgtatg aaaaaaagaa tagatttatt attagtagaa caagggtact ttgaaagtag
    32821 agaaagagcc aaaaaagcaa taatggctgg attggttttt gttgataatc aaaggtgtga
    32881 taaggctggt acagaagtaa aagaagattg tagtatagaa gtaaaaggaa atccaatacc
    32941 atatgtcagt agaggtggtt taaagcttga aaaagcaatg aaaaactttg atttgactat
    33001 tgatggaaag gtatgtatgg atataggggc atccacaggt ggttttacag attgtatgct
    33061 caaaaatgga gcaataaaag tattttctat agatgttggt tatggtcaac ttgcatggaa
    33121 attgagacaa gatgataggg ttgtgtgtat ggaaagaacc aatataagaa atgtgacaat
    33181 agaagataca aagcagtttg ctgattttgc gtcaattgat gtgtctttta tatcgttaaa
    33241 gttagtactt ccaaaggcaa aggagttagt acgtcatgat ggggaagttg tcgctttaat
    33301 taaacctcaa tttgaagcag gaagagaaaa agtaggtaaa aaaggagtag ttagagaaaa
    33361 atctacacat atagaagtta taaaaatgat ttctgatttc tcagtcgaaa atggatttga
    33421 gattttaggg ttagattttt ctcctattaa aggtcctgaa ggtaatatag aatatctaat
    33481 tcatttgaga aatggaaatg aaggatatga atttgatggt gagacataca ataacaaaat
    33541 agtagaagtt gttgaagctt ctcataattt agacaaatag gttaaaaggg ggcttagatt
    33601 tgatcctcga attatatatg aaaaattgtg ccttagttga agaactaagg ttgaatattg
    33661 ataaaaactt aaatatactt acaggtgaaa ctggttcagg aaaatctata ataatagatg
    33721 cacttggact ttgtttaggt gataaatatg ataggtcttt tttaagaaaa ggaacagata
    33781 agggacttgt tgaggctgta tttttttcag acaatagata tcttaaaaaa atattagaag
    33841 aaaatgatat aagtatggaa gatgataatt tattggtaat aactaggttg atatactcag
    33901 atggtaagag tactgctaga gtaaatggaa ggactgttaa agtgtctctt cttaaagaaa
    33961 ttgcatctac acttatagat atacatggtc aacatcaaaa tcaagctttg tttaataaag
    34021 atactcattt aaaatttcta gacctttttg gagaaaatga attggaagag tttaaaattg
    34081 cttataaaaa tgtgtaccat aagtatagtg aagtaaaaaa agctttaaat tgcttgacag
    34141 aaaataaaga tgagatgcaa attcaacgag aaatagattt attaagattt caaataaatg
    34201 aaattgaagc tgcaaatcta aataagaatg aatatgaaga tttattaaaa caaagagaag
    34261 tttatagaaa tagtgaaaag atatataata atctaaattc gagttatagc aagttacata
    34321 atggtgagta taatgttatt gacctaatag gtcttgcatc taaggaactt aatgacatat
    34381 ctaaatatga cagtgtttta agtgaatata gtgatactgt agaaagaatc atgtatgagt
    34441 tacaagacat atctggtgag ataagaaatt acaaagataa tatagacttt gaaccttatg
    34501 agctagagca gattgagctt agaatcgacg agattaataa cttaagaaga aaatatggag
    34561 acagtataga tgatatattt gagtattatg gaaaaattaa agatagatta gatgaaatat
    34621 taaacagaga tgaaagagtt gaacagttaa gaagcaaact gatgaatata gaagaagatt
    34681 taaaaataaa agccagtaaa ttgactaaag caagaataga agtagcaact actttagaaa
    34741 aaatacttct tgatgaatta aaaagcttaa atatgaaaaa tgtaatgttc aaggttaatt
    34801 ttgaaaaagg tccatttact ttagatggag tagatgatat tgaatttatg atatctttta
    34861 acttaggaga agatattaag cctatatata aagttgcatc aggtggtgag atgtccagat
    34921 ttatgttagc gtttaaaacc atattggctg atatagatga tattgataca ttagtgtttg
    34981 acgagataga tactggaata agtgggatag cagcacagat agttggtgag aaattgagtg
    35041 atatagcaaa gaaaaaacag ataatatgta taactcattt acctcaaatt gctgcaaatg
    35101 cagacactca ttattgtata gagaaagata cttcaaataa taggacgttt accaatgtta
    35161 gtaagttaaa tgaatctcaa cgaaaaaatg aaattgccag acttatagca ggtaacaata
    35221 taacagaaaa aactattgaa catgcaagtg aaattattga gttggctaaa aaatgttagt
    35281 gaaaaacaag cattttaata ggtgtaaaga atttttatag aaatgcttta catctatttt
    35341 ttatgatgaa attattttaa aatatctagt gcctatattg aagaaaagta gatttaaaaa
    35401 tagaagaaaa tttgtataaa tttgtaactt tgaaatttct aaatgcttaa gtataatatt
    35461 atttattaag gtagtagtga gggggacata aaatgaataa aaaagatact atgcacttaa
    35521 tttttacaat tgtaattttt attttattat ggtatattgt ggtagcagta agtgagatta
    35581 ataaaaatat tgaaagttgt agtaataaat tagattattt gtcacaagag attatgagta
    35641 ctaaatcgga tattagtaat actataaatg aagaaatgtc aaaaagttat ataacaaaga
    35701 gtattgactt aaaagtaaaa aaaatcgaga aaaaagagtg tgttattgat gtagatgttc
    35761 aattaagtaa acttggaaaa aatggaaaag tattttttat gtataaagaa gatagtgata
    35821 aatggaatga aactgagatg acaaaacatg gagaattatc atatgcatgt gaaattaaga
    35881 taaatacagg aagtgaatat gactataaag tagttacaag tggagctata tctgaaagtt
    35941 ctgatgtgca aaaattgact aaatctgatt atatgcctga acaacccata tttaattctg
    36001 ggataagaga taatagagaa tactttattg aaatagtaga aaatagtaat cttttcaatc
    36061 atgagagtga aaagtcaaaa aataaagttt atgataatat tagattagaa aaagtagata
    36121 ttattgtaaa tgaaggaaaa aaagacacta tatacaaagc caaattagct gaaggcttag
    36181 atgatggcaa aactaataat tcaaatagaa atcaagtgaa ttatgaagta agttttccta
    36241 aaagggatat tgatgacata atttatatta aagctaaact tacatacaat aatggagtag
    36301 aatatacaaa ggatataact gaagatataa ctatgggatt acaagattta gaaaaataaa
    36361 tacatattta aataaatatt caaatagatt tgttcaataa aatagagtat aaataaaact
    36421 gtagtagttt tacttatact ctattaaaaa attataattt taattccata tatctagccc
    36481 cttcaattgg attataagta taaggttcta tatcataaaa accaaacttt tcatataatc
    36541 cttgtgcact tttcatgctt ggaagagtat ctaatctcat gtgtgtatat ccaaattttt
    36601 tagcttcttc aatgatttct tccaatagta tttttcctat ttttaatcct ctaaattgat
    36661 ttcttacata taatcttttt aattcacata ttttaccttc aagttttttt aaagcaacac
    36721 aacctgccaa attttcatca acaaatgcca atatcaatga gcctgaaggt tttttatatt
    36781 ttcctggaag tgtttttaat tcattattaa agtcttgaaa acataagtct atatttagag
    36841 aatttgaata ttcagtaaat agaagtttta cgttttcaag gttttctaaa tctttttctt
    36901 caacaaattg taaagttata ttttgagcca ttttatccgc cattatataa acatgtttat
    36961 ataatgactt cacctccttg atatatgtat atttttctta atatcaatta gattttaata
    37021 ccattacttt ttcaccacca tagtcaagct ctccattaaa ctcaaatcca aaatctttat
    37081 aaagcttaat agccatttta ttgtcttcga atatactaag atatagttca tcacagttat
    37141 attcattttt aagatgattt acaagaaaat ctaatgatgc ttttccatat cctttacctt
    37201 gatattttga atcaatcata aatctatcaa gccaaactct atcatcttct aaacgaccat
    37261 acatagcaaa tcctattaaa gaattttcat catatattcc aacaggtacc caagctgtta
    37321 taaatttaga ttcagcaata gaaagtgcat tgctttctat ataatctaac tgacttttat
    37381 ctacagacaa actaactaaa tcatgccaat tatttatgtc tgcaattctt acatttaatt
    37441 tcaagatatt accgcccttc aaactttttg ttacaatcta atactatcat attatgtatt
    37501 aaaaatcact tattaagatt ttaaataaac tttttaaatg aataaattct caggaaaaag
    37561 gaattataaa tacataataa tacagcatag gagtagatgg atatatgaac aaaataaata
    37621 aaaatttcaa aaatatttgg ataatattag gagctgtatc gatttttacc atggttataa
    37681 tttttaataa aaattgtaat gttaataaag agactcaaat agaagctttt acaattaaga
    37741 atcatgataa aaaatttgta tatcctatgg gaaatataat tggagtaaaa gcaaatacag
    37801 atggtgtatt ggtcttagga tatgaggaag aggatgtaga ctatattgga ggcatacaaa
    37861 tcggtgataa tatagtaaaa atagataata aaaggataaa aaatagtcaa gatgtatcag
    37921 agattttgaa taagattaaa aaaagtaaag ttgaagtgac ttttgagaga aaaaataagt
    37981 ataaaacaga agtaatagag actaaaaaag aaaatggcaa gtataaactt ggattatggg
    38041 taagagataa aatatctgga attggaacta tgacttttta cgaccctagt atggaaaagt
    38101 tcaaagcaat cggtcatgcc ataaaagatt cggatactaa tgaactatta aagataaagc
    38161 aaggatatat atataagcca gaacaattaa aaatagtaaa agctagtaat gaaaaagtag
    38221 gtaaaataaa aggagatttt aatgacagca atttaatggg taatttctca aataattcag
    38281 agttaggtat aagtggtaat attacagaaa accataataa agagtttaat gtagcaaata
    38341 aagaaaagca attaatagaa gttggaagac cgcaagatgt aaaaatagga gatgcagtaa
    38401 ttctttttga ggataaaaac aaaaatataa caagctatga tataaaaata gaaagtatag
    38461 tatatgataa aggaaattat agagatatgg taataaaagt agtagatgac aagttattgg
    38521 aatacacagg aggtatcgta caggggatga gtggagctcc aataatacaa aataataaaa
    38581 ttattggtgc aataactcat gtttttagag ataatccgaa aaaaggatat ggtattttta
    38641 tagatgaaat gataaaattg taggtgaggc attaaaaaat tttattattt tatcaattat
    38701 ctaggaggaa tataattttg gagtgtcgaa tatgctttag agtagataat taggaagcaa
    38761 ttgtgtaaaa agtttagttt tctgtaataa gaagatgttt tttaatgggg ggatttttag
    38821 tggaaaaaat caaaatagtt ttagcagatg acaataagga tttttgtcag gtattaaaag
    38881 agtatttgtc taatgaagat gatatcgata tattaggcat agctaaggat ggaattgaag
    38941 cattagactt agtaaaaaag acacaaccgg acttattaat actagatgta ataatgccac
    39001 atctagatgg attaggtgta attgaaaaat tgaatactat ggatatacct aaaatgccaa
    39061 aaataatagt actatcagca gtaggtcaag ataagataac tcaaagcgca ataaatctag
    39121 gagcagatta ctacatagta aagccatttg attttgttgt gtttataaac agaattagag
    39181 agctagtttc taatagagtt acacaagttg agccaaaacc tagaccagtt caagagactc
    39241 aaatgactag aagtgatttt gttaagaatg taggaaatat agagacagaa atcacaaata
    39301 taatacatga aataggagta ccagctcata taaaaggata tttatattta agagaagcaa
    39361 taaaaatggt tattgataat gttgagcttt taggtgcagt aacaaaagag ttatatccaa
    39421 gtatagctaa aaaattcaat acaacaccaa gtagagttga aagagcaata agacatgcaa
    39481 tagaggttgc atggagtaga ggaaaagttg acacaataaa tcaattattt ggatatacgg
    39541 tacacaatac taaaggaaaa ccaactaatt cagaatttat agcaatgatt gctgataaat
    39601 taagattaga acatagtatg gttaaataaa caagacataa aaagtaaggc tttttaatta
    39661 aggcattggc tataaatgcg tattacaagc agcgaaacgg tataaccact agggttatac
    39721 cgtttcgcta ttttaataaa tataaaaatt ttctttatta tttgcttact atatcaatat
    39781 aataatttta ttatactatg gatatagtat gtgtctttac aagttgtaaa ctgacagtgg
    39841 tttatttttt aatataaata ttgactttga tgcaggtaaa ctttgtattt ttaagcgtat
    39901 tatggaatat gttaaataaa aaaatgatga aatatagtat tgtaaatgcc aaagatgcaa
    39961 aacaaactta aaacatttat tttattgtta agtaatgcta taatataatg tgattttaat
    40021 aatgatagtg gaggtttaaa tatgagagtc gaggccccta taaaagtaga tcgaaaaacc
    40081 aaaaaacttg ctaaaagagt tgaaagtggg gaaatagcag ttataaatca tatagacata
    40141 gatgaagttg ctgcaaactc tttagtagaa gctaaaataa aacttgtcat aaatgcggct
    40201 ccttctataa gtggtaggta tcccaataaa ggtccaggga tattaactga aaataatata
    40261 ctaataattg ataatgttgg agaagagtta tttgaagaat taaaagaagg agaaactata
    40321 gaagttgtag atggaaagat atatagaaaa ggaaaatttt taggtgcagg agaagtttta
    40381 gacaagtatg aggtttcaca ccaaataaag gctgcctatg agaatttagc tgtagaattg
    40441 gatagattta tagataatac tatagattat gctaaaaaag aaaaaggatt tatacttgga
    40501 gaagtagaaa ttccaaaagt gaaaactaat tatgctaata aacatgtatt gatagttgta
    40561 agaggtcagg actacaaaga agatttaagt actatgcttt cttatataga agaggtaaag
    40621 ccagtattgg ttggtgttga tggaggagca gacgctttaa ttgagtttgg atatactcca
    40681 gatgtgattg taggtgatat ggatagtgta agtgatgaag ctttaaaaaa ggctagtgaa
    40741 atagtagtac atgcttatac agatggaaga gcaccaggtc ttaagagggt tgaggaatta
    40801 ggcttagatg cagttgtatt tcctgcgcca ggaactagtg aagatatagc tatgcttata
    40861 gcctatgagt ataaggctga gttaatagta gcagttggta cacattcaaa tatgattgat
    40921 tttcttgaaa aaggaagaaa aggtatggca agtacttttt tagttagact aaaaataggt
    40981 tcaaagctta tagatgctaa aggcgttaat ttgttataca gaagtaaatt aaaaataaag
    41041 tacatatggg cattaatagc aacagcatta tttcctgtgt tggtagttgc atcattgtca
    41101 ccaggagttc aacagttcat acaactaatg caattaaagt ttagagtatt attacagatg
    41161 taaattttag gaggataaat atgcatatta atatgaaata ttatatagtc actatagggg
    41221 ctatatttat agctttgggt ataggcatgt tagtaggatt taatttaaat aataatcaac
    41281 aattaagtga acaacaagca aatataataa atgatttgga tgataagttt aatatcttaa
    41341 aagaaaaaaa tgataaactt gacagtgact tagcaagtgt aaatagagat tatgaagagg
    41401 cagttaattt tataaataaa aatgtagata agattttagc tggttcgctg aatggtaaga
    41461 gtataggaat aatatctgct aatgaaaatg acgattatac aaaaaatata gaagacataa
    41521 taaataaatc taatggaagc attgcattta atataatatt aaaagaaaac ataacaaatc
    41581 cagaaaagtt aaaggaaatg tcaactaaac taggtacaga tgtaaaaaat gcaaatgatg
    41641 cagttaatta tataattgac actcttaaaa aggaagatgc aagtgacatt ttaacatatc
    41701 ttcaagaact agatgtaata aagtttaatt ttattggaga tacatatcta aaatatgatt
    41761 ccgttgtaat agctggtgga aatgatgcaa aagacagtac aaaacagttt gaaaaaatag
    41821 aaaaatttgt agtttcaaaa cttaaatcag agaataaata tttagtagaa gttcaaaata
    41881 caggagttaa aacttcttat gtagaactat attctaaaaa caaagttgca actatagaca
    41941 atattgatga aggtatagga agtatatctc tagcgattct tctacaacaa ggtaatatag
    42001 ttggtaattt tggtagattg gatactgcaa cttcattgtt gccatctatt aaatagaaat
    42061 aattttaagg agagaaaaaa tggattctta tataagtata ataataccag cttataatga
    42121 agaaagtaga ataaaatata cattagaaag catattggac attaagcaaa taaatgaaat
    42181 aatagttgtg gatgatggtt caattgacaa tactgctaag gttgtatctg agctagaaaa
    42241 tgaaaagatt aaatttttta agttagataa aaatagaggt aaaggatatg ctttaaatta
    42301 tggtatcaag ttagcaatga aaaatgcaga tattataggt tttctggatg gagatttagg
    42361 aagttctgct aaagaagttg aaaaattaat agtacctata ttaaatggtg aggcggatgt
    42421 tacaatagcc aagtttcctc ctgcaaagag aaaaggaggt ttgggatttg taaaaggttt
    42481 agctaaaaat tctgtgtttg agatgactgg tgttgagtta gatgcaactt tatcaggtca
    42541 aagaatattt aaaagagaag ttttagaaaa gtttgatgaa ataccttttg gatatggagt
    42601 agaagttgga atgaccattg atatattaaa gtatggattt aaaattaaag aagtattagt
    42661 aaatatgact cataatgaaa caggaagaaa tttaaaaggt tttattcata gagggaaaca
    42721 gtattatcac ataaaaaagg ttttaggaca aaaaaagaaa gaatggaggt aatatcttag
    42781 tgaaagatta tatactttat accattttat tattaactgg attatttgga acctatgctg
    42841 taattccact atttaaaaat ttattgataa atggaaatgt acttagacca aactataaaa
    42901 aagatatgat accagtaagt atgggaattg tatttttgcc tatgattgta atcaatggaa
    42961 taatacttgc tttctttact acagaattta agaatttagc gtatatattt atgtttatgt
    43021 ttggtatgat atccatgttt tttgcaggga tactagatga tgtcattgga aatagagatg
    43081 taagtggttt aaaggggcat tttaaaagtt tatttaaagg aagtcttaca acaggaggtt
    43141 ttaaagcttt atttggtgga tttgtagggc ttatagtttc tgttgctata tcaaaagaca
    43201 taattgatat aatagtaaat acactaataa tagctctatc aactaattta atgaatcttt
    43261 tggatttaag accaggaagg gcaataaaag catatttatt cataatggta attgtatttt
    43321 taacattgac aggatttgtt caagttcttc cattacttat agtaccaaat gtacttgcat
    43381 actttaatta tgatttaaaa gcaagggcta tgatgggaga tactggttca aacgtccttg
    43441 gaatatctat tggaatgctc atttcttttg ggtatccatt taacattaga ttaggatggt
    43501 taatatttgt agttattatg catatattca ctgagaaata ctcattaact aagataatag
    43561 aaaaaaataa actattaaat tttatagata aattgggaag gtagtattat gataagcaaa
    43621 agagtgggga ttgttgaatc aatagtaagt caaactgaaa ctctagatga tataagagta
    43681 aatataaatg gagaaataca aaaggcatat aattatccaa aaatatctgg aactataaat
    43741 ataggtgacg aagtggtttt aaatacaaca gcagtagaac tgagcttagg tacaggtgga
    43801 taccattttg taataactaa tttaaataat atagaatcta ctttaactga aggtggacat
    43861 ataatgaaac ttagatatac gcctcttcaa ataaaagtgg attctgttga ggaacaagaa
    43921 agtatttatc atgataaatt tgcgaatttt aaaggattag aaggtctttc tgttgtagta
    43981 gggactcttc atagtatgtt aactccattt gcagcttcat ttaaaagaaa taatccaaat
    44041 aaaaaattag tgtatataat gactgatgga gcatctcttc caatttattt aagtaaaaat
    44101 gtggatactt taaaagaaaa aaaattaata gatagtacaa taactatagg aaatgcgttt
    44161 ggtggagatt atgaatgtat aaatatatat actgcattaa ttacagcaaa agaaatacta
    44221 aaagcggatg cagtatttgt cagtatggga cctggaattg ctgggacagg aacaaaatat
    44281 ggatttacag ggattgaaca aggctctatt cttgatgctg taaaaaaact tgaaggaaga
    44341 gcaattgcaa ttccaagaat tagttttgct gacaaacgag aaagacatca aggtatttca
    44401 catcatagta tgactgtatt taaagaaatt gtaaatgtaa atgtagacat accaataact
    44461 atatatgatg atgaaaaatt gaactttata aaagagcaaa tcaatttaaa tggtttagac
    44521 aaaaagcata atataattta tatagataat aataaaacga aagaggattt agactatttt
    44581 aatctaaaag taaagagtat gggaagaaat tatgaacaag atgaggcatt tttcaaagct
    44641 gcaagcacag cagcatatta cttaatggag gtttaagatg gtattagaag aaaaaactat
    44701 tagtagtgat agggtttata caggtaaagt aattacttta aaggttgata ctgttgaaat
    44761 tccagggcaa ggatatcaaa aaagggagtt agtagaagtt ggtggagcag taggaatagt
    44821 tgcaatcaca gatgataata aagttgtgtt agttaagcaa tttagaaaac ctatagaaaa
    44881 gccaatcttt gaaattccag ctggtaaact agaaaaaaat gaaagtccaa aagaatgtgc
    44941 tgaaagagaa ttaaaagaag aaactgggta tagtgcaaaa aatattaagc taatacataa
    45001 atttttcact tcagcaggtt tttctaatga gataatgttt gtatatttag caacaggtct
    45061 tactccagga gagaataatt tagatgcaga tgagttttta gatgtttatg aaattgagct
    45121 tgaagaagca tataacatgg tattaaaaaa tgacgttgaa gatgcaaaaa catctattgg
    45181 attattatta gtaaaagata tgtttaaaaa ttaatattta taaaatgaat aaccctttgt
    45241 cctaaagcat attattttat aaaggacaag ggggaattaa attgagaaga acatatagaa
    45301 aaaatgattt tagagaatta aatagacaaa taattattat aggattgttg tttatgatgt
    45361 ctattgttgt tgggtcttac ataaataaaa tattgccagg ttctagtaac aatatattaa
    45421 acaatataaa tcctgctgta gagtattata atctaaatat aagcataaaa gatacagtga
    45481 tacaaaattt aaaatcagat gcaatcttta tgggctcaat agctctatta agtctatttg
    45541 tagtaacaat tccagcagtg ttggtagctt ttgtattaaa agggatgtca attggttata
    45601 caataaatag ttgtatattg gcattaaaat ttaaaagtat aaaaatgatt ttaataatat
    45661 tatttaaaaa tttgattatt attccatgtg caatcatatt agcattaatt agtttaagtt
    45721 attttaaaga aatggtttat gaatttaaaa agaaaagtag aaaaaatatg caatttttaa
    45781 ttaagaggta tatactaaat attataatta taatagcact atcattgggg ttgcaattaa
    45841 tattaaatac tgcaagtatt ggtattatta agttcctagc taaataaagg agtttataat
    45901 ggatattata gagggatata tagattacat aaaagataaa aaaaaattat cagagaatac
    45961 agtggcatca tactttatgg atataaaaaa atatatggaa tatataaatc aaaaaagcat
    46021 taaactagta gatatagtag aaaatgatat tataagttac ttaataaagt tggaaaaaga
    46081 taatgtatca atagctacaa tagcgaggat gatatcctct ataaaatctt ttcatgatta
    46141 tttattttta aatcatatat gtacaaataa tcctgcaaag gatataaaaa aacctaaaat
    46201 aaaaaaagaa aatataaata tacttacaga agaagaaatt gaaagactat taaattttcc
    46261 aaagttaaca acacctaagt tgattaggga caaggctatt tttgaagtat tatatggtac
    46321 tggtataaaa gtatctgaat tagtagagat gaatatagag gatattgacc tagatattga
    46381 ttatatatat tgtaattcat gttgtaaaaa tcaaagagtg ataccacttt gtgatattac
    46441 aaaactttac ttagaaaagt atctaaaaga agcaagacct aaaatggctg cagaaggtga
    46501 aaaatcatta ttcgtaagtt cattaggtca aaggtttaca cgacaaggtc tttggaaagt
    46561 cataaaaaaa tattctaatc tagccaatat agataaaaat ataaatccta caatgcttag
    46621 acattcattt gctattcatt tgttaaatga aggtgctaac attgctgttg taagtaaaat
    46681 tttaggaaat gttaatttat ctagtttgca agtatattta aatcatatag ataaaaacgt
    46741 gagaagagaa ataaaggaga aacatcctag aaatgatgtt gatgtagaat tacaaaaagc
    46801 agaagctaaa tagactatat agcaaatttt ataatttaga aggagcatat ttatatgagc
    46861 agagtaattt ggattgtaat tgatagtgtt gggataggag cattacctga tgctgaaaaa
    46921 tttggtgata gtaaggatgt aagtaccctt ggaaatatat ttaaggaata tccagatatt
    46981 cagattccta atatgagaaa tttaggtata ggaaacatag aaggtataga tttttttgaa
    47041 agtataaaag aaccaatagg ttgttttgga aagtgtaagg aaatgtctca gggaaaagat
    47101 actactacag gtcattggga aatgactggt ataatagttg acaaaccatt taagacattt
    47161 gaacatggat tctctaagga gataatagaa gagtttgaga aaaaaactgg aagaaaagta
    47221 gtaggaaata aaccagcttc aggaactgta ataatagatg agtatggaga gcatcaaata
    47281 aaaactggag atgtaatagt gtatacatca gctgatagtg tatttcagat tgctgctaat
    47341 gaagaagtaa ttcccctaga agaactctat aatatgtgca aaatcgctag ggaaataatg
    47401 atgggggata atgcagtagc tagagtaatt gctagaccat tcataggtaa gaaaaaaggt
    47461 gagtttgtga ggacttcaaa tagaagagat tattcactag acccatttga accaacagtt
    47521 cttgataata taaaagaatc aggacttgat gttttagcag ttggaaaaat agaagatatt
    47581 tttaatggaa aaggaattac agatgctatc cacactaaaa gcaatatgga tggagtggat
    47641 gaaactttaa attatatgaa acaagataat aaaggtctaa tatattcaaa tctcgttgat
    47701 tttgattcta aatatggtca taggagagac ccagaaggat ataagaaagc tcttgaagag
    47761 tttgatagta gacttcctga aataatggct aatatgagag aagatgatat tttaataatc
    47821 aatgcagacc atggaaatga cccaacatat aaaggtacag accatacaag agaatatata
    47881 cctgttatga tttatggcaa taagattaaa aaaggtttta atttaggagt aaaagatact
    47941 tttgctgata ttggagcaac tgttgcagat atactaaatg taaagttgcc taaacatggg
    48001 tcaagcttta aagaagattt attttaaact ataaaatgat aaaaacttag cttaatagga
    48061 tggagagatt ggaatgtttg aaaagatagc acaaagtagt aaatttataa attctaaaag
    48121 taatataaaa cctaagattg gtttgatatt aggttctgga ctaggtgatt tagcaaatga
    48181 tatagaagac cctgtcacaa ttaaatatag tgaaattcca aacttccctg tatcaacagt
    48241 agcaggacat gctggacaac ttgtaattgg aaaacttgaa ggaaaagaag ttattgctat
    48301 gcaaggtaga tttcattact atgaaggata ttctcagaaa gaagctactt ttccagtaag
    48361 ggttatgaaa gaattaggag tcgagattct tatagtaact aacgctgctg gaggcgtaaa
    48421 taaagaattt aaagcaggag atttgatgct tataagagac catataaact ttagtggaag
    48481 taatccatta gtaggaaaaa atgatgacag atttggtgct agatttccag atatgtcaga
    48541 tgcatattct tctaaatatg tagatgtagt taaaaactgc gctaaagaat gcaatataga
    48601 tgtcaaagaa ggcgtttata tgttttttag tggtccaaac tatgaaacac cagcagaagt
    48661 gagaatggca caaattttgg gagctgatgc tgtaggtatg tctactgttc cagaagtcat
    48721 agtagcttct cactctaata taggagttat tggtatatct tgtataacaa atatggctgc
    48781 tggcatctta aatcaaccac taagtcatga ggaagtaata gaaactactc aaaaagttaa
    48841 atcagaattt atgaatttag ttaaaacaac ggttaaaaat ttataagtta aagcttatta
    48901 tagatttttt gaggaagttt ataataaaat agttaatgtt taatttgaaa agaattaata
    48961 aaaagctgtc ttgatataga aagttatttc taataaagga cagttttttt ataatactac
    49021 aacatttgaa agtgttttaa agtttaatat ttatttaaat tattataggt tagaatggag
    49081 atttcaatta tgagaatgta tgatattata aaaaaaaaga gagataacct agagcttagt
    49141 aaaaaagaga taaactactt tatagaaaac tattcaaagg gagatatacc tgactatcaa
    49201 gcttctgcac tattaatgtc gatttattta aataagatga ataaacagga aactgtacat
    49261 cttacagaag caatgatgaa ctctggagac atgataaacc tatctgagat agatggaata
    49321 aaggtagata agcatagtac tggtggtgtt ggagataaga ctaccatagc tcttatacct
    49381 ttggttgctt catgtggagc accagttgca aagatgtcag gtagaggtct tggtcatact
    49441 ggtggtacta ttgataaatt ggagtctata ccaggttttt cgacagaaat ggagataagt
    49501 aaatttattg attctgtaaa taagtctaaa atagcagttt gtggtcaaag tgcaaaggta
    49561 gcaattgcag ataagaaaat atatgctctt agagatgtta cagcaactgt tgataatata
    49621 tcattgatat catcaagtgt aatgtctaaa aagttagctt caggagctga tgcaattgtg
    49681 ctagatgtaa aaactggtga tggagcattt atgaagacat tagaagagtc ttttgagtta
    49741 gctaaatcta tggttgatat aggtactggt atgaatagag atacaatagg gattgtaaca
    49801 gatatggatg aacctttagg ttttgcagtg ggaaactctt tagaagttat agaggcaatt
    49861 gagacattaa aaggaaatgg acctaaagac cttgttgagc tatgcgaagt attaggagca
    49921 tatatgttgg ttttatcaaa ggtagcacat gattttgaag atggtgttga aaaaataaga
    49981 gaatcaataa aatctggtac tgctatacaa aaactcaaag agtttataga gaatcaaggt
    50041 ggtaataaag atgtagtaga tgattattct ttattcaaaa aatcaaagta taaatatcca
    50101 gttaaatcaa ctaaatcagg ttatataagc aaaattaaag ctgaagatgt tggtgtatcg
    50161 gctatgattc ttggagcagg tagagaaact aaagatgata ttttagattt atcagcaggg
    50221 ataattttag aaaagaaagt aggagattat gtaaatgaag gtgaagtttt agcttacatg
    50281 tattataatg atgaacaaaa gctttcattg gcaaagggaa gatttattga ttcctactct
    50341 attgtagatt caaaaataga aaaaaataaa ttaatatatg gtattgtaac taaagaagag
    50401 attaaaaagt tttagaaaat tttagaaaag tgattataaa aggcctaaca tgtcaaaaat
    50461 aatgttaggt tttttatttt ttgataaact ataaattata ccaaaaatat atattttaaa
    50521 acatttttta caatataaat aagtttattc tacaagatga ttgaaaatta aagtatctag
    50581 ttttataata gaaaatggat aaatatacat aaggagtaga ttatattatg aactttaaag
    50641 aaaatagatt gaaaatagca gtattaataa ttgttatttt gattatctta gcaggtatat
    50701 ttgtgtttat acaaataggt ccttatgata aaaataacaa aaaagatgta attatcgacg
    50761 ttccaagtgg agcatctgtt ggaaaaatat ctgacatatt atatgaaaat aaattaataa
    50821 agaatgaact attatttaaa cttttggtaa aggttagcaa taaagctccg tctataaaat
    50881 caggaacata cttacttaat caatcgtatt caaacaatga tataataagt cttttagttt
    50941 caggtaaaat atatcaggat ggtataaaag tgactattcc agagggagct acatctaaag
    51001 aaattatagc aatgttagta agtaaaaatt taggagataa agctactttt gaaaatttaa
    51061 taaaaaaacc tcaagaattt tatgataaat tttcatattt gaaggaagat ggaataacct
    51121 ctttagaagg gttcttgtat ccagaaactt attattttaa ttcaaagaaa caatcagaag
    51181 aagatatttt aagcgaaatg ttaaaagtat ttgacagtaa atacacggac aagtttaaaa
    51241 agaaacaaaa agaattaaat atgactcttc aagaagtaat ggaaatggca tcaataattg
    51301 agaaagaagc tgttcttgac aaagacagac ctataatagc aagtgtgttt tataatagac
    51361 ttaaggtagg tatgcctctt caatcagatg ctacaataca atatatattt gaagagagaa
    51421 agaaaattgt aacatatgat gatttaaaaa ttgattcacc atataatagt tataaaaata
    51481 agggattacc tcctacacca atatcaaatc ctggtataaa atctattgaa gcagcattat
    51541 atccagagaa aacagactat ttatattttg tagctaagat tgatggtgga aataattata
    51601 gtacaaacta tcaagaccat ttaaaatacg ttaaagaata taaagaagca agggataaac
    51661 aatcaaaaga tacaaaagca acaaataaag aaaatacaaa aaaataaacc atgattataa
    51721 aaagtctaac agggtaagaa taatgttggg cttttttatt gaaaattgtt attcttagat
    51781 ttataataaa gaaagtgtaa atatatataa caatatgaat ttaaaaaata ataaattaat
    51841 ataaaacttg gatatttatt attttaggga gtgtttttat gagtaatata gttaatgatt
    51901 tggttgaaga ttatataaga gtgacattaa aagaaaaaga aggattttta aaagatttag
    51961 aaatttatgc agaggaaaat agtgttccta taatacataa agaagtttca gatttattaa
    52021 aagtcttgtt aaaggttcaa aaaccaaaga gaattttaga agtaggatgt gcaattggtt
    52081 attcttctat tttttttgca actattatag gaaaagatgc agatataata actgttgaga
    52141 gaaatgaaaa aatgatagaa aaagcaaaag aaaacataaa attagctgga tttgacaata
    52201 acataactat actagaagga gatgcagaag aaaaacttag tcaagtccaa ggtgaatttg
    52261 accttatatt tatagatgca gccaaaggtc aatataagct attttttgat ttagttatag
    52321 ataaattaaa agatggggga cttttagtat ctgacaatat tttgtataaa ggtatggttg
    52381 ctcatgatga ttttgttgta agaagaaaaa agactatagt taaaagaatg agaaagtatt
    52441 tagattatat atgtaattgt gactatttaa gcacatcatt ggtaccaata ggtgatggtg
    52501 tagcactaag ttataaagaa agtaaaagag gtgatgtaga tggaattaaa taaagtagaa
    52561 cttttagctc cagcaggtga tttggagaga ttgaaaatag ctataacata tggagcagat
    52621 gccgtttata taggtggaga aatttttgga atgaggtctg cagcgaagaa ttttagtaag
    52681 gaagatatgg cagagggagt agcttttgca catgaaagag gcaagaaagt attcgtaaca
    52741 gtaaatataa tacctcataa tgaagatttt ctacaattag aagactattt gttggaacta
    52801 gaagagatag gaatagatgc agttataata gctgacccag gagtgcttag tgtcattaaa
    52861 aaagtaattc cgaatatgga aatacattta agtacacaag caaatacaac caattatcta
    52921 agtgcaaact tctggtacga gcatggaata aaaagagttg tagtggcaag agaattatct
    52981 tttgatgaaa tatatgagat aagagcaaaa acacctttag atatggatat agaagcgttt
    53041 atgcatggtg ctatgtgtat ttcttattct ggcagatgtt taataagcaa ctatatgact
    53101 ggaagagatg caaacaaagg ttcttgtgct caatcttgta gatggcaata tcatttggtg
    53161 gaagaaaaaa gacctggtga gtattttcca atatacgaag atgaaagggg tacattcttc
    53221 tttaactcaa aagatttatg tatgatagag tatataccag agttaatcaa atctggtata
    53281 acaagtttaa aaatagaagg tagaatgaag actgcatact atgtagctac tgttgtaaga
    53341 gcatacagaa tggcaataga tgaattttac agagacccag aaaactggaa gtttaatcca
    53401 atgtggatgg aagaactaaa aaaaggtagt catagacatt ttacatcagg tttttacttg
    53461 aataaaccaa caacagaaga ccaaaattat caatcagcat catatgttag aaactatgat
    53521 tttataggta tagttagaga aacagaagat gaagatggtc ttattgttgt tgagcaaaga
    53581 aataaaatgt gtgttggtga tgaaatagaa gttatgggac catataaaga aactatgttt
    53641 actaaaattg aagaaatgta taatgaagaa ggagaagcta tagaatcagc tcctcatcct
    53701 agacaaatag tgaagttaaa gctatcagta aaagttggta aagattatat gcttagaaaa
    53761 gttatagaag aaaaggttga agaataataa aaaattgata aaaatagtat tagcccctta
    53821 gaaattgtca ataatgaaat tatctaaggg gtgatttcat gtcaaaaaag aagactccat
    53881 ttttgaaaaa agtgggaaag agaagctggt gtatatttac tataatttta ataatttata
    53941 gtgttttaat ttataggcta gttgatatac aagtattaaa aggtgataaa tacaaacaaa
    54001 gtgttgaatc acaaagcgtt gagaaagtcg agttaaatag tggaagagga ataatctatg
    54061 atagaaacaa caagaagctt acagatacaa gtaaatctca ggtattaatt gttgaaaaag
    54121 aaaaattaaa taataactat aaaattttgg agcttattaa gaaagctact aagatgaatg
    54181 atttagatat atataaagct gtacaagagc aattgacacg ccctataata cagattcaga
    54241 ctaaaaatat cgatatcgat aagagtatga aaaaagaact tgagaaaaat ggaataatgg
    54301 tagaagaaaa aactatgagg tacgcaaaag atggtttatt atcacataca ataggatata
    54361 taaaagaaga tgataaatca ggtcaatcag gaattgaaaa gagtatggat agtgtactta
    54421 gaaattcaaa tgaaaagtat ataagtgctt ttaaagctgg agatgcagga aatgaaaaat
    54481 ctctaaacat actaaaggga agtgtaaaaa ctgttgataa taaggataaa gatagacatc
    54541 taaaaactac tattgattat aacattcaaa aaaaattaga acaaatacta aataaagaag
    54601 aaaatcctac agcagcaata atctcggaag catctacagg tgaaattctt gcaatgtgct
    54661 ctagacctaa ttttgaccaa aatgacatat caaaaagttt aaaaggaaaa aatggagaat
    54721 ttgaaaatag agttataaaa gcgacatatc caccagggtc tgtatttaag atggtagttt
    54781 tattttctgc acttgaaaat ggtgttatag atgagaacta tacttacaac tgtacaggta
    54841 agactaaagt tgggaatact aatgaaatac tgcgatgtaa taaaagagat ggtcatggtt
    54901 tccaaaactt aagacaagct ttttctaatt catgcaatcc tgcattttta gatatagcta
    54961 tgaaacttgg aaaagaaaag atactcaaat ctgctgaaaa attacattta tttgaaaagg
    55021 ttgatattgg tcttgatgaa gaaaaaatta gagaagctcc aaaaaacata tcaataagaa
    55081 atttagctat aggtcaagaa aatatagaat ttacaccact tcaaataaat caaatgactc
    55141 aaataatagc taataatgga acattcaaac cactatactt atataaaagt cttgtggaca
    55201 ataatatgaa tacaataaaa acttataagt cttcaaaaaa agaagaactt atttctcctt
    55261 atgtgtgtac acaagttaaa gaatatatga agtctgtatc taggactggt acagctaaag
    55321 acctaaaaga tattgaaggt ggttgtggag ttaaaactgg tacagctcaa agtagtttaa
    55381 ataaaaaagc tatagaccac ggatggataa caggatttta tcctgaagaa agacctaagt
    55441 atgtaataac tgtgttagta gaaggaactc aaaaaggaaa taaatctgca acacctatat
    55501 ttaaggaaat atgtgaaagt ataaaataaa ttatgtacta taaaatttaa ggatatatcg
    55561 taactttgat tttagataga aaattttgtc taatgatgta cgatatatcc ttttaaaata
    55621 tagtattaac tttcttgaac aagctctttt tctagttttt taagagcttt tttttcgatt
    55681 ctagatacat atgaccttga tatatcgagt ttttgtgcaa tttctctttg tgtcttatag
    55741 ccataaggag ttaacccata tcttaactgg acaatttctc tttctctagg agtaagaatt
    55801 ttgtttagtt gttcgtaaag ttttccaact tgaactttta gttcaacttc atcaagaaca
    55861 tagttagcct ctgtaccaag tatat
//
LOCUS       DAYAOX010000002        47580 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.002, whole genome shotgun sequence.
ACCESSION   DAYAOX010000002 DAYAOX010000000
VERSION     DAYAOX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.002"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     regulatory      72..162
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            571..888
                     /gene="flgB"
                     /locus_tag="ACGI57_000059"
     CDS             571..888
                     /gene="flgB"
                     /locus_tag="ACGI57_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFG9715151.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            896..1300
                     /gene="flgC"
                     /locus_tag="ACGI57_000060"
     CDS             896..1300
                     /gene="flgC"
                     /locus_tag="ACGI57_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFG9715152.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1310..1627
                     /gene="fliE"
                     /locus_tag="ACGI57_000061"
     CDS             1310..1627
                     /gene="fliE"
                     /locus_tag="ACGI57_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFG9715153.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1652..3202
                     /gene="fliF"
                     /locus_tag="ACGI57_000062"
     CDS             1652..3202
                     /gene="fliF"
                     /locus_tag="ACGI57_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFG9715154.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3215..4285
                     /gene="fliG"
                     /locus_tag="ACGI57_000063"
     CDS             3215..4285
                     /gene="fliG"
                     /locus_tag="ACGI57_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFG9715155.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            4269..5009
                     /locus_tag="ACGI57_000064"
     CDS             4269..5009
                     /locus_tag="ACGI57_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HFG9715156.1"
                     /translation="MTLLNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFR
                     DEVIKEAIESAEIRKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYT
                     QKAKDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESM
                     SDLLKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGN
                     AIIQKENGRIIVGIDCAMEKLKEELLGE"
     gene            5013..6332
                     /gene="fliI"
                     /locus_tag="ACGI57_000065"
     CDS             5013..6332
                     /gene="fliI"
                     /locus_tag="ACGI57_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFG9715157.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            6364..6810
                     /gene="fliJ"
                     /locus_tag="ACGI57_000066"
     CDS             6364..6810
                     /gene="fliJ"
                     /locus_tag="ACGI57_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFG9715158.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDNVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6838..8043
                     /locus_tag="ACGI57_000067"
     CDS             6838..8043
                     /locus_tag="ACGI57_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFG9715159.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNNKFEDNLYKTTDHKNV
                     KDRRETSKQNVSSDRPGNSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNLELNGANTNNPNDIE
                     NLDIMKLRELLENNASEDIQVISKTTENDTKSIDLYSFDSNRMDTLNSNKQKDDSSDI
                     LEKLAGLNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSNQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            8061..8669
                     /locus_tag="ACGI57_000068"
     CDS             8061..8669
                     /locus_tag="ACGI57_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFG9715160.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEDEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVDVKYEKLLKIGEVEANSGEENDSSEQESPEEV"
     gene            8696..9679
                     /locus_tag="ACGI57_000069"
     CDS             8696..9679
                     /locus_tag="ACGI57_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HFG9715161.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9692..9895
                     /locus_tag="ACGI57_000070"
     CDS             9692..9895
                     /locus_tag="ACGI57_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HFG9715162.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9895..10713
                     /locus_tag="ACGI57_000071"
     CDS             9895..10713
                     /locus_tag="ACGI57_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HFG9715163.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEINEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10703..11398
                     /locus_tag="ACGI57_000072"
     CDS             10703..11398
                     /locus_tag="ACGI57_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFG9715164.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKEDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11410..11853
                     /gene="fliL"
                     /locus_tag="ACGI57_000073"
     CDS             11410..11853
                     /gene="fliL"
                     /locus_tag="ACGI57_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFG9715165.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11868..12242
                     /locus_tag="ACGI57_000074"
     CDS             11868..12242
                     /locus_tag="ACGI57_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HFG9715166.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12243..12908
                     /gene="fliP"
                     /locus_tag="ACGI57_000075"
     CDS             12243..12908
                     /gene="fliP"
                     /locus_tag="ACGI57_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFG9715167.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12921..13190
                     /gene="fliQ"
                     /locus_tag="ACGI57_000076"
     CDS             12921..13190
                     /gene="fliQ"
                     /locus_tag="ACGI57_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFG9715168.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            13202..15016
                     /locus_tag="ACGI57_000077"
     CDS             13202..15016
                     /locus_tag="ACGI57_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HFG9715169.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLINNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFIDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPIAMTLLAFTLLVPTLFSYVVNTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15025..17100
                     /gene="flhA"
                     /locus_tag="ACGI57_000078"
     CDS             15025..17100
                     /gene="flhA"
                     /locus_tag="ACGI57_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFG9715170.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17078..18091
                     /gene="flhF"
                     /locus_tag="ACGI57_000079"
     CDS             17078..18091
                     /gene="flhF"
                     /locus_tag="ACGI57_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HFG9715171.1"
                     /translation="MTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFSK
                     KDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLNQ
                     EIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTIA
                     KLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEMK
                     DCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRNV
                     NFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLGV
                     ESI"
     gene            18088..18966
                     /locus_tag="ACGI57_000080"
     CDS             18088..18966
                     /locus_tag="ACGI57_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HFG9715172.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVGVCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18980..19681
                     /locus_tag="ACGI57_000081"
     CDS             18980..19681
                     /locus_tag="ACGI57_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9715173.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDTEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19697..20119
                     /locus_tag="ACGI57_000082"
     CDS             19697..20119
                     /locus_tag="ACGI57_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HFG9715174.1"
                     /translation="MIQIILIVVSILIIVTIVINLQKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            20104..20289
                     /locus_tag="ACGI57_000083"
     CDS             20104..20289
                     /locus_tag="ACGI57_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HFG9715175.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20330..21103
                     /locus_tag="ACGI57_000084"
     CDS             20330..21103
                     /locus_tag="ACGI57_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFG9715176.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21124..21885
                     /locus_tag="ACGI57_000085"
     CDS             21124..21885
                     /locus_tag="ACGI57_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFG9715177.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21908..22789
                     /locus_tag="ACGI57_000086"
     CDS             21908..22789
                     /locus_tag="ACGI57_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFG9715178.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNECIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22792..23166
                     /locus_tag="ACGI57_000087"
     CDS             22792..23166
                     /locus_tag="ACGI57_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFG9715179.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23198..24601
                     /locus_tag="ACGI57_000088"
     CDS             23198..24601
                     /locus_tag="ACGI57_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFG9715180.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYNSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24717..26654
                     /gene="htpG"
                     /locus_tag="ACGI57_000089"
     CDS             24717..26654
                     /gene="htpG"
                     /locus_tag="ACGI57_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFG9715181.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            27042..28172
                     /locus_tag="ACGI57_000090"
     CDS             27042..28172
                     /locus_tag="ACGI57_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFG9715182.1"
                     /translation="MNFNYNLPVNILFGSGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            28399..29421
                     /gene="splB"
                     /locus_tag="ACGI57_000091"
     CDS             28399..29421
                     /gene="splB"
                     /locus_tag="ACGI57_000091"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006290 - pyrimidine dimer repair [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HFG9715183.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29603..30514
                     /locus_tag="ACGI57_000092"
     CDS             29603..30514
                     /locus_tag="ACGI57_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFG9715184.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30529..30945
                     /locus_tag="ACGI57_000093"
     CDS             30529..30945
                     /locus_tag="ACGI57_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HFG9715185.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(31045..31437)
                     /locus_tag="ACGI57_000094"
     CDS             complement(31045..31437)
                     /locus_tag="ACGI57_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9715186.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31578..31973
                     /locus_tag="ACGI57_000095"
     CDS             31578..31973
                     /locus_tag="ACGI57_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFG9715187.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            32376..32582
                     /locus_tag="ACGI57_000096"
     CDS             32376..32582
                     /locus_tag="ACGI57_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715188.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
     gene            complement(32894..33637)
                     /locus_tag="ACGI57_000097"
     CDS             complement(32894..33637)
                     /locus_tag="ACGI57_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HFG9715189.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            33803..36370
                     /locus_tag="ACGI57_000098"
     CDS             33803..36370
                     /locus_tag="ACGI57_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9715190.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            36591..37004
                     /locus_tag="ACGI57_000099"
     CDS             36591..37004
                     /locus_tag="ACGI57_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9715191.1"
                     /translation="MKLVIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNI
                     EDLNKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLE
                     ALINREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            37028..37525
                     /locus_tag="ACGI57_000100"
     CDS             37028..37525
                     /locus_tag="ACGI57_000100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9715192.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            37528..37929
                     /locus_tag="ACGI57_000101"
     CDS             37528..37929
                     /locus_tag="ACGI57_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9715193.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            37947..38435
                     /locus_tag="ACGI57_000102"
     CDS             37947..38435
                     /locus_tag="ACGI57_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFG9715194.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            38462..39217
                     /locus_tag="ACGI57_000103"
     CDS             38462..39217
                     /locus_tag="ACGI57_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9715195.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            39217..40080
                     /locus_tag="ACGI57_000104"
     CDS             39217..40080
                     /locus_tag="ACGI57_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9715196.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            40127..40606
                     /locus_tag="ACGI57_000105"
     CDS             40127..40606
                     /locus_tag="ACGI57_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HFG9715197.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            40671..41978
                     /locus_tag="ACGI57_000106"
     CDS             40671..41978
                     /locus_tag="ACGI57_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9715198.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            42306..42428
                     /locus_tag="ACGI57_000107"
     CDS             42306..42428
                     /locus_tag="ACGI57_000107"
                     /inference="COORDINATES: protein motif:HMM:NF021468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFG9715199.1"
                     /translation="MLSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDENGE"
     gene            42938..44032
                     /locus_tag="ACGI57_000108"
     CDS             42938..44032
                     /locus_tag="ACGI57_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9715200.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            44037..44237
                     /locus_tag="ACGI57_000109"
     CDS             44037..44237
                     /locus_tag="ACGI57_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFG9715201.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            44234..45154
                     /locus_tag="ACGI57_000110"
     CDS             44234..45154
                     /locus_tag="ACGI57_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9715202.1"
                     /translation="MMNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKT
                     TTMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCG
                     EITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDE
                     PTSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLS
                     EIKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTV
                     ISTLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            45151..45909
                     /locus_tag="ACGI57_000111"
     CDS             45151..45909
                     /locus_tag="ACGI57_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFG9715203.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTIYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            46157..47230
                     /locus_tag="ACGI57_000112"
     CDS             46157..47230
                     /locus_tag="ACGI57_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFG9715204.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            47300..47563
                     /locus_tag="ACGI57_000113"
     CDS             47300..47563
                     /locus_tag="ACGI57_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715205.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
BASE COUNT        19177 a         5015 c         8712 g        14676 t
ORIGIN      
        1 aaaaataaaa tatttaatta tacacaaaaa agatatttaa aaaatttgtt atttattgta
       61 tacttaagtt aaaactaaat aggcaaatct agagaaatct agtgacgcaa agctataggg
      121 actaaggttt atatacataa actatgtcag ccagttgcca aaaagagtcc taggtgtatt
      181 gtatacctaa gaaaagtcta taatgactgc ctttttggca gtcattttgt tatggaaaaa
      241 tcactttatt tcagtagaaa atacacataa taacatattt taccaattat aacataaaat
      301 aaagaaaaat tatctcaaaa agttactatt ttacaaaaaa tgacttcttt atttatttaa
      361 attaatcgca tttccattac attaatacaa taatgtatca atttagtaag aaaactaata
      421 tttttttaat gaagtcccat gaaaatataa aaaaatttta atatgtattt aaatatttct
      481 tcgaaaagtt gctctttttt gtaatatagc aacttcattt tttttattaa taaacaaaat
      541 agataaaata tattttgagg aggggtgagt gtgaatacat atgacattat aaaaatggga
      601 ttagatgcaa ctaatctaag aagtcagaca atagcaaata atatagcaaa tataaatact
      661 gaaggatata aaagacatta tataactttt gaagaaacac taaatgatgc tatgcctaaa
      721 atagaagaaa aaacagacaa ttctaaaggt ttaagagtag atggtaataa tgtagacata
      781 gaagaagaaa aagtaaatca agcaatggca acattacaat ataatgcttt gataagtttt
      841 gcaaatggaa aaatagcaat gaccaaatca ataataagtg ggaggtaatt agaagatgag
      901 tgtgttcagt ggaatgagaa tatctgctag tggattaaca gcagaaagtt tgaaaatgga
      961 tatcatatca tccaatgtag ctaatataaa tgctacaaga ggtccaaatg gagaagcata
     1021 taggagaaaa gttgtttcat ttgaagaaaa ctatgataat aaattaggta tgctaggagt
     1081 aaaagttaca tctgttgaag atgataaatc acctcttaaa gaagtatatg acccatctca
     1141 tccagatgca aagccaaatg gatatgtaga atatccaaat gtaaatatat tggtagaaat
     1201 gacagattta ataacagctt cacgagcata tgattctaac gtagatacat tgaatgctca
     1261 gaagtctatg atatcaaaag ctttggaaat aggaaagtag ggataaatta tgccaattac
     1321 acaaattaac aatttaatgc aaggtgcaaa gttagataat ttgattggag aagcaaagcc
     1381 attaactctt aataataaag aagaaaaaaa gaattttgaa gatgtaataa tagatactat
     1441 agataaagtt aattttcaac aagtaacagc agatgatatg aaagaaaaat ttataaaagg
     1501 cgaagatgtt tctatgcatg aagttatgtt aataggtcaa gaagcacaac tatctatgca
     1561 atatttaata gaagttagaa ataagattca tgatgcatat caagaaatga ataaggtgca
     1621 aatttagcct tttgaacagg agtaataatc aatgataacc agttttaaaa ataagattaa
     1681 ggagcaagtt gctaaatcta ggacaaagat aagttcttta aaaaagggaa ctaaaatagc
     1741 attaggtata ggggttattg ctatatgttt agcgctagta tttacagcaa tatctgcgag
     1801 caaaaataag tatgcagtct tattttcaga actagatgct caagatgctg cagatatatc
     1861 agcagagcta gagtctaaaa agatagatac taaggtaaaa ggaagtacga tttatgtacc
     1921 tagaacagaa gttgataagt taaggctgga gttagcgcca agtcttacag gtggttctaa
     1981 aggatttgca ctaatggatg atagttcatc aatagggctt actgatgaag aatttcagat
     2041 taagaaacaa agaatgatac aaggtgaaat agaaaaaaca ataaagagtt ttccacaagt
     2101 ggaagacact agagtacata taacatttgg agagtcatca gtatttgaga aggaggctgt
     2161 tccagggagt gcagcagttt atttgatgct aaaaccaggg acgactttag aaaaaaacca
     2221 aataaagtct gttatggcac ttatatcagg aagcgttaca aatataccag agaaaaatgt
     2281 tcaggttatt gataacaaaa tgaatttatt atctgaaggt attttggagg atgaaaatgt
     2341 tgatgaaaat aatcaaccaa taaatagtgg cattagtgtt gataaaaata aggcatctga
     2401 acaagcattg aataaacaac tagaaaagtc tattttagaa atgctagaac cgatatttgg
     2461 taagggaaag gtgaaagcta cagtaaatgc tgatttaagt tttgatacag ctgaaaaaac
     2521 tgagataaaa attgaccctg ataaagttgc aataaaggag acaaggtcga aaaattcatc
     2581 taaatctgct gcaaataatg ttcaaggtgt agatgctaac atgaataacc aaggaaataa
     2641 caatggtact aatacagaag atagtttaga tgagagtttt gagtatgaaa caggtaagat
     2701 tgaaactcac actatagttg cgcctggaga acttaataga ttgacagctt ctgttgcaat
     2761 agatggaaaa atagctaaag gtgttcaagc tgatgtagag gatattgtta ataatgctat
     2821 aggtatggat ggagcgaggg gagatactct tagcgttgtt tcaatggtat ttgacccaga
     2881 aggtaagaaa gaggctaaac aagagctgga agagattaaa aaagaaggat ttaaaaagaa
     2941 cataataaaa gcagttattg gagtagtagc agtaatagca gttggagcta ttggatactt
     3001 aatatataag aaaagaagtg aacgagaaga tatggattat gaagatgaag attatatttc
     3061 taaatctgat tctgttgata ggaaggataa tcctctatta ggagatgacc cagaactgac
     3121 attagaggaa gctgttaaaa tgtatgcaga agaaaagcca gaccaagtaa cagagataat
     3181 aaagacttgg ttaaatgagt aagggggata gaaagtgtcc acggaaatta caaatgttaa
     3241 tgcgtttgac ttaagtgata taaatgaaag tgataagaat gagaaggtag aaggtgaggg
     3301 tttaaaaaaa gctgctgtat tattaatgac attaggccca aatgtgtcta gtgatataat
     3361 aaaaagcttg cctgataaac aagttcaaaa aataggtttg gaaattgcaa atatttcttc
     3421 aatagcttca aaagaaagaa gagaaatctt aaaagagttt gtagaaataa atagggcaaa
     3481 agattatgtt atagaaggtg ggcttgaata tgcaagagag ttattaagta atgctttagg
     3541 aaatgttaaa gcaaacaaat tacttgaagg tatatcaata aatacaagta caaagccttt
     3601 taaacatatt ataaaactag atgtaaagaa gatattagaa atacttagga atgaaagtgc
     3661 acaaactata gccgttttac ttgcacatat ggaaccagaa aatggagcag aagttttatc
     3721 atacttagat tttgatttac aaaatgaagt agctatgaga ataagctcaa tgggttcaat
     3781 atctccagaa gtaataaagg taattgaaag ttcattagaa gataaattac attcaataag
     3841 tggtgatgat tttaatgatt atggaggggt gtctacctta gttcaaatat tgattaattc
     3901 tgatagaaaa acagaaaaga atattttggg aactattgaa gaacaaaacc cggatttagc
     3961 agctcaaatt aaatctcata tatttgtctt tgaagatatt gtcaagttag atgatataag
     4021 catacaaaaa gtgttaaaag aagtgactat gaaagattta gtatatgcct taaaaggtgt
     4081 agatgatgag atttcagatg ctatatatag aaatcaatct tctagagcag cagatgcact
     4141 taaagaagaa atggaaatgg ttggtgttat aaaggttaca caagttgagg cttctcaaca
     4201 aaagatagct aatatcgtaa gaaaacttga aagagaaggt gccataacta ttgtaaggaa
     4261 ttttggtggt gactttgttg aataataaga ttattaaaag taaaaaagca ttatataaag
     4321 gagtgctaga tttaaccata atagataaag ttagagtggg agaatcaaat gtaaataaag
     4381 aaaatgattg tagatttagg gatgaggtaa taaaagaagc tatagaatct gctgagataa
     4441 gaaagaatga gatattagaa gaagcaaaaa tgaatgcaca aaatatagaa aagcaagcat
     4501 atgaaaaagg gtatagtcaa ggtcaaaaaa atggatatga agatggatat aaagagtcat
     4561 atgatgaata tacccaaaaa gctaaagatg aagcagattt tataaaaaat caggcagact
     4621 taatactatt acaagctaat gagaagatga ctgagtacat aaaagaaaaa aataaagaga
     4681 ttgtaagact tgctattaat atggctagtt ctgttctaaa aaagcatttc gaagatgaag
     4741 aatctatgag tgatttgtta aagcaaatta ttcaagatta tgaaggaagt agttcattta
     4801 tagtaagatg taattctttc tataaagata atatagagaa agagtttgaa ttattcaaag
     4861 aggaaacatc actaaaatca agtgtatttg ttattgcaga tgatagtata gataaaggca
     4921 atgctattat ccaaaaggaa aatggaagaa taatagttgg tattgattgt gctatggaaa
     4981 aactaaaaga agaattgctt ggtgaataaa gtatgtataa aactgatata tctatagatt
     5041 ttgacaaaat aaataagaaa atagaaaatt tagaactcac tatatcagaa ggtagagtaa
     5101 agaagataat aggacttact gtagaagttg aaggaataaa agcttttgtt ggggagttat
     5161 gcgttatata caaccaagtt aataaaccag tgaattgtga agtagtaggt tttaaagata
     5221 aagaagtcat attaatggcg ttgggagaat taacattgat agcacctggt tgtaaagtta
     5281 tttctaaggg gataccttta agtgttatgt gtagtgataa tttactaggt aaagtacttg
     5341 atggtcttgg aaatcctata gacaactcag atgctgtttt aggagataga tacaacctga
     5401 acaatgaacc tcctgaccca atgaagagaa aaaagatacg aaatataatg gaaacaggtg
     5461 taagagcaat agatgcattt actacttgtg gtgaaggaca gagaatcggc atatttgcag
     5521 gaagtggagt aggtaagagt acgactctag gtatgattgc taggaatgca aaagcagatg
     5581 taaatgtaat tgctcttatt ggagaaagag gaagagaagt actagatttt attgataaag
     5641 atttaggtga agagggaatg aaaaaatctg ttgttgtgtg tgcaacatca gataaagccc
     5701 ctctagttag gttaaaaggt gctcttactg ctactgcaat agcagaatat tttagagacc
     5761 aaggcaaaaa agtaatactt atgatggatt cagtaactag atttgctatg gcacaaagag
     5821 aagttggtgt agcaatagga gagccaccag ctcaaaaagg atatacacca tctgtatttg
     5881 caatgttgcc aaaacttatg gagagaacag ggacatcaga taaaggttct ataactgcat
     5941 tttatacagt attagttgat ggagatgact ttaatgagcc aatagcagat acaacaagag
     6001 ggatacttga tggtcatata gttttatcta gagacttggc aaataagaat cactatcctt
     6061 ctatagatgt attaaacagt ttaagtaggc ttatgaatga aattgcttca aaagaagaca
     6121 taaaaatagc gtcttttgca agagacatgc ttgctgagta tagagaagca gaagatttga
     6181 taaatatagg tgcatatgct tcaggtacaa acaaaaaaat agatgaagca atctattatc
     6241 atgagcatat tattaatttt ttaaaacaag gaataaatga aaagagttct tttaatgaaa
     6301 ctataagtag tttaagaaga atctttgaat aaacaattta aaaaaatatt tgaataggtt
     6361 gtgatggaat tggataaaaa ttttaagttt agattacaga aagttttaga cttaaaaata
     6421 aaagatgaag aagagataaa aatggaattt gctaagattc aacaaaaaaa aatagatata
     6481 gaaactaatt tagaaaactt agaaagtaat tacagcaaat attctataag taaaaataat
     6541 gataatgttc aaaatcaaaa aataactatt aactatttgc tagctttaaa caactctata
     6601 atggatttat cagaagaact ggataagtcg acaaatgaac ttgaaaaagc tagaaaacag
     6661 cttataagca aacagataga gagaaagtca ttagaaaaat taaaagaaaa aaaatatgga
     6721 cagtattata aagaagaaca gttaaaagaa caaagcacta atgatgagtt tgcaagcatg
     6781 agttatttga gaaatagaca agttctataa aataatagga agtgaggata ttattgtatg
     6841 aagatacaag cagattctta taatattatt aaagatattg ggaatagaaa aaactcatct
     6901 aagaataaaa atataaaaaa taataaattt gaagataatc tatataagac aacagaccat
     6961 aaaaatgtta aagatagaag agaaacaagt aagcaaaatg ttagttcaga tagaccggga
     7021 aatagtgata atgagtgtca tttagatgaa gataaaagta atcacttaga agatgaaaat
     7081 acatatgaaa aagattgtag taagaataat gaagctttac agcaattact gaatctttta
     7141 aaagagaatt ataaaaaaga taaagatata aacactatat tgaataatat aaaaaaacta
     7201 agtatatcag atgaattaaa acaagaactt aagcttaata aaaatatatt gaataaaatt
     7261 gcaaatcaaa atttagagtt aaatggagct aatacaaata accctaatga tatagaaaat
     7321 ttagacataa tgaagttaag agaactttta gaaaataatg ctagtgaaga tattcaagta
     7381 attagtaaaa ctacagaaaa tgatactaaa agcatagact tatacagttt tgatagtaat
     7441 agaatggata ctcttaactc aaataaacag aaagatgata gttctgatat tttggaaaaa
     7501 ctagcagggt taaatggaaa taagagtgga aattttggac aagtaataaa taaaagcgtt
     7561 gatatgaata aaattaaaaa tgataatgtt aatttaaaac agatagaatc taatgtaatg
     7621 gatgattcta taaaagctat taagcatatg aaaacaaatg atatacaaga attgacaatt
     7681 aaattaagac caaaagaatt aggagatatg aacatacaat tattaaaaga tggtgaaagt
     7741 atgagagctg ttgtgactgt tttcaataaa gatgtctttg actctataaa taaaaacata
     7801 gcagatttaa agcaacatct tgaacttaca aatgtaaata taaaagatgt atcagtgcaa
     7861 atgcatagcg ataatacaaa tacatctgat acctttgata aagcatttga acaacagaat
     7921 agacaaaata accaagaaaa catgagtaat caaaatacaa atgataagcg taacaatgta
     7981 gtcatagaag atgagattaa agaaaatatt attgatgatg atagagtaga tttactagct
     8041 tagtaagggg gaagtattgc atgtacggaa tagcaacggc tttggcagga actcaggtaa
     8101 actataggtc aaataactta agccaacttg aaagaaatat taatgatatt gccaacaaac
     8161 aagttagaag taaaggaaat cagacagtaa cagacaatgg gactccaatt ataggacaag
     8221 gtgatgatgc tgataaagac ttgtttatga agattcttgt agcacagatg ggtaatcaag
     8281 acccaatgaa tccacaagac ccaacggagt atgtatcaca attagcacag tttgcaactt
     8341 tagaaaaaat gtcagctatg aatgataagc tagatatact tttagcctta agtaactcta
     8401 ctctaataaa ctctgctttg agtatggcaa catcattaat tggtaaagaa gtagaagtat
     8461 ctggggaaga agatgaagat gaaaacattg taggagtagt aaagtccaca tacataaaag
     8521 atggggaagt acacttagaa ttagaagtag atggaagtga caaaactgta gatgttaaat
     8581 atgaaaaatt acttaaaata ggagaggtag aggcaaattc aggagaagaa aatgactctt
     8641 cagaacaaga atctccagaa gaagtataat attaaatggg aaaagaggta aatttatgat
     8701 aaaagcaatg tattcaggtg taagtggaat gaaagcaaat caaacaaaac tagatgtaat
     8761 cggtaacaat gtagccaacg ctggaactac ttcttttaag aaaagttcag ctagaataac
     8821 agattcattt tatcaaactg tactatatgc aagtgctcca acagcggcat tagggggaac
     8881 aaacttagga caagtaggtg taggtagtaa gatatcaagt ataaacaaag atatgacaca
     8941 aggaaatgta caaccaacta atagaccatc tgatttaatg atagatggag atggatatct
     9001 tccagtagta agacaaggaa cagttatgta tacgagagat ggttctttta atttagatac
     9061 aggatttaaa gcagctgatg gtgctttgaa gaatattaaa ggtggaagac ttgtaaatgg
     9121 tgatggatat atgttacaag gtatgattta tgatggggaa tataaagctg atactggaga
     9181 atttgttaaa aagggtgatg ctaaaggaaa agagcctatt gtaataccac ttcaaagtat
     9241 aagtcctaag gatggaacag ttcaaaatgt actagagtat aatatatcaa aggatggaac
     9301 agtagaattt ttattgagtg atggtcaaag aactcaattt gtctatgata tacctgatgg
     9361 tgctaaggta ggagatacac ctgctggaat agataaatta aaaggtacaa tacaaaaagt
     9421 gcaaacatat gcattccaaa atccagcagg actagatgca gaaggaggaa acttgttcaa
     9481 gccatcagca aactcaggag acccacaaat agcaggagct acaggcacaa taatacaagg
     9541 agctatagaa gcttccaatg tagatatagc agaagaattt acagaaatga taatagcaag
     9601 taggtctttc caagcaaaca gtaaaataat atcgacatca gacgaaatac ttcaagaaat
     9661 aataaatctt aagagatagt aggtacatat tatgataaga gtaacggatt taaacggaag
     9721 aaaatacata ttaaattcag atcttatagt gagaattgac tccgtgccag aaagcaggat
     9781 tctccttaca acaggagaaa aaaatcttgt aaaagagtca gtagatgaga tagtagataa
     9841 agtaataaag tataaaagaa aaataaatgt aggttatatt aggagtgaaa aataatgaaa
     9901 aaaacagatt cactaacatt cattggacta atattatcta cagtattagt tttatttgga
     9961 gcagtaaaag gaagtagttc aggattgaaa agttttttcg attttgcatc tatattaatc
    10021 acagtatttg ggtcatttgg agctttgatg attacattta ctattgacga tataaaactt
    10081 ataaaagatg ctctacaata ctcattcaag acaatgaata tatcgaaatt agacttatta
    10141 aatcaattta aaacactttc taaaaaagca agaagagaag gacttttatc tatagaaagt
    10201 gatgtaatga aaatacaaga cccatttatg aaaaaaggat tagagttatg tattgatggt
    10261 cttgaaataa atgaaataag gtctatacta gagctcgaaa tagattctgt tgaagaaacc
    10321 aataacagat attctaaact atttaaaact tgggggactt tcgcacctgc atttggtatg
    10381 ttgggaactc taataggttt aatacaaatg ttatccgacc ttacatctcc agatttaata
    10441 gcatctggta tgggaaaagc attaattacg acattttatg ggtctttact tgccaatatc
    10501 gctttgaacc caattgcata taatatagat gagaagactg aaaaagaaat ttatgtaaag
    10561 gaaatgatgc tggaggggat aataagcata caatctggag agagcagcat agtagttgag
    10621 gaacgtttag ctacatacct ttcaaataat gagaagttag aaattatgaa atccaataaa
    10681 aatacagaaa gagcaatgag taatggcgcg taaaaaaaat aagaaaaagg atgagataaa
    10741 tccagatgca tggttggcta cttatgcaga tacgataaca ctgattctta cattttttgt
    10801 gttattgtat gctacatcag ttgtagatat ggaaaaattt aaaaatatag cagatgcgtt
    10861 aaaaggtcag ttttctggga caagtatgtt tagtacaggt tcaagtacaa gtgagaaatc
    10921 tccaatagag agtgttttag tggataaaaa tgataagtca aatttagaac aggatttaaa
    10981 agataaagtt aataattcta acttacaaaa tagtgtaaca gtcaaagatg atagtagagg
    11041 gattttacta gagctagatg attcaatttt atttgactct gggatttctg aattaaaaga
    11101 agatagtaaa gccactctta ataaagtata cgatatgata aagatgatga aaaataagat
    11161 agttatagaa ggccatactg ataatgtacc tgtaaaatca agtacgcatg aatccaattg
    11221 ggatttatct tctagtagag cagtaagtgt tgtaagatac tttgtagaag aaaagggcat
    11281 ggaccctaaa ctattttcgg caacaggatg tggagagtac caacctttgg tatcaaatga
    11341 aacagcaaag ggaagagctg agaatagaag agttaatata cttataacta ttgactagga
    11401 ggaagaggtt tgttcaaaaa taaaaaaatt attataatta ttttgatact gattattttc
    11461 tcagcaggag caggtttttt tatattagga gaaggtggaa ataaacaaat tgacaagatt
    11521 gcaggtttgt ttattccaac agggaaaggc aaagatgaag taaagaccga aaatcttgac
    11581 ttagaagaga tggtattgaa gctagctgat gaaggtgtaa gatatttaaa agtatcaata
    11641 actgtatctt atgattcttc atataaagat atagaaaaaa atacgccttt aattagggat
    11701 aatattataa attgttttat ggcaaaaagg gcagatgatt ttactgctga taatttaaag
    11761 aacataaaag aagaaattaa aaatacatta aatatacaac taggtgaaaa tgtaattcaa
    11821 aatatatatt tcacaaatat agtagtgcaa taaaaggatg gtaagacatg atactttatt
    11881 tactaaaaat attaatatgt atcccaataa ttttaatatt gatagtctta tctttaaaaa
    11941 ttagccaagg aactttatta aaaaaatata atgataaata tataaaaata ctagatattg
    12001 taaatataag taaaaacaat agcataataa ttttaaaaat aggtgaagaa ggatgcatta
    12061 tggcatcttc ttcttctagc atgagtaaaa taaaagattt gaccaaagaa gaaatattaa
    12121 gaatagaaaa cgacattaaa gaaagaaatt ttaaaattca agattttaaa atggaaagaa
    12181 ttaaaaagaa atatgatttc agtagatata ttggaaaatt tacaaaaaag gaagataagt
    12241 aaatggaact attatcaaaa ttacctttgg ctgatgtacc atatacaagt tcaatgcaat
    12301 tgtttttatt tctaactgca ttaatgtttt taccatttgt aatgttcatg atgacaagct
    12361 ttgtcagaat agttataagt ttatcatttt tgaaatcagc attaggagca cagcaagcaa
    12421 ttccaagtca aattttggta gggcttgcaa tagtattgac aatatttata atgagaccag
    12481 tattaaatga gataaacgaa aaggcattac aaccatatat gaaagaagaa gttactatgg
    12541 aagaagctat gaaggaagca gaaggaccta ttaaagaatt tttattaaag caaaccagac
    12601 agacagattt agatttattt gtagagcaag ctggtttaaa agaaaagaaa ttaactcgtg
    12661 aaaatatacc attatctgtt gtagttccag catttgctat aagtgaacta aaaactgctt
    12721 ttcaaatagg ttttttaata tatataccat ttttgataat agaccttgtt gtggcaagtg
    12781 ttttgatgtc tatgggtatg tttatgttac ctccagttat gatatcatta ccatttaaat
    12841 tattattatt tgtaatggtg gatggatgga acttgatagt aaaaacattg atattgggat
    12901 ttggatagga gaaaaatagt atgagtgaat ttatgataat gagtgttctt aaaaatggca
    12961 tgtttacagg agctatgatt gcagctccag tgctaattgt ttctcttgta gtggggcttt
    13021 taataagtgt atttcaagcc acaactcaaa tccaagagca aactttgact tttgtaccaa
    13081 aactaatagc aattctttta gtagtagcta ttggtggaag ctggatgtta catactctta
    13141 ttagctttac ttcggagata ttcaatatga tgtctctcat aactaaataa gtaggatata
    13201 tatgattttt gtatttatta gaatagtagc attctttgga acattaagga tactatttcc
    13261 agctggtaca cctgctttat ttaagtcgct ctttgcaatc attatatcaa tcttgatttc
    13321 aagtactatg caaattgaat atgcaacaaa tatagacaac attgtattgt ttaccttata
    13381 tggtataaat gagaccataa ctgggattat gcttggatat ataaccaatc tatgttttta
    13441 tagtatcaga atggctggtt ctatgatgga ccaacagatg ggattatcaa tgattaatat
    13501 gtttgaccca aattcaatga ctcagacaac tttaatcaat aatcttatga actggacagc
    13561 acttatgata ttttttagca tggatggtca tcatgtttta attagaggca taaggtatag
    13621 ttttgagtta attccaatag ggaaatcatt catagataat aatatagatt atataataaa
    13681 catatttgtg cagtgctttt taactggatt caaaatagca atacctattg tactttgtct
    13741 gcttatggca gattttatat tagggcttat atcaagaagt gtacctcaac taaatgtaat
    13801 gataattggt atgccattaa aaatactggt tggtattgca ttatttatca tatcaatacc
    13861 tttaattgtc aatcaaataa gtcatttatt aagtcaaata ccaaagatgt atgagggaac
    13921 ttttgcacta gctcctatgt tttttatggg ttcaacagat aaaacagaag aagctactcc
    13981 aaagaaaaag ggagaacaaa gaaaaaaagg taatatagca aagagtaaag aattacccat
    14041 tgcaatgact ctacttgcat tcactttatt agtacctaca ttattttcat atgtagtgaa
    14101 tacacttaaa tcttctctta attatttttt gagtctagat ttttatatga acataaatta
    14161 ttcaagtata gagaaattag ttatagcagg attaatggat ttttttaaga tatttttacc
    14221 aatagctatt ccattcttag ttttaggtat agtcgcaaac ctatttcaag taggaatact
    14281 gtttactggt gaaactttaa aacccaattt atcaaaatta aatccaatta gtggattcaa
    14341 aaacatgttt tctatgagaa gtttgtcaac cttaataaaa gatacagcga ttatatctat
    14401 tttggcttac ataggatata cattcttcca agataattat ttagatatat taaagttagg
    14461 taacatatac ctgccaactt taatgtacac agttaaagat ttagtatatt caatattaag
    14521 taagatttgt gtagctatga tagttatagc agttgcagat tatgtttatc aaagatacag
    14581 tcataaaaag caacttagaa tgacaaaaca agaagttaaa gatgaatata aaaactcaga
    14641 aggagaccca gaaataaaag ctaagataaa acaaaaacaa agacaaattt cgtctcaaag
    14701 aaccatgcaa gcagtaccaa gtgctactgt aatagtaacc aatcccaccc acttatctat
    14761 agcagtgaga tatgaaaaag ggaaagacca agctccagta gtggtggcaa aaggtgcaga
    14821 ttatttagct tttaaaataa gggaaattgc aaagggaaat gacataccta taatagaaaa
    14881 taaacctatt gctaggttgt tatataagca agtagaaata gaccaagaaa ttccagagga
    14941 catgtatcaa gcttttgcag agatattagt agcagtatat aaaatcaaaa atagatacaa
    15001 agtccccaaa aggtaggata agtaatgaat aagtttaatt taaaggaaaa tatggacgta
    15061 atagtggcat ttggtgttgt agggattgtt cttatgatga tagtgccgtt acctacattt
    15121 atattagata ttttactggc aattaacata agtttatctg tgctaatttt actaatgaca
    15181 ctatttacaa caaatgtact tgatttatct atatttccaa cagtgttact tgtgacaaca
    15241 ctatttagat tgggattaaa tctttcatca actagattga ttttaacaca gggatatgca
    15301 ggtgaagtta tagaggcatt tggaagtttt gttgtaggtg gaaattacgt agtaggaata
    15361 attatattct taattatatt aattgttcaa tttgttgtta taaccaatgg ttctggaaga
    15421 gtatctgaag taacagctag atttacgtta gatgccatgc caggtaagca gatgagtata
    15481 gatgcagatt taaatgcagg agttatagat gacaaaactg ctagaaaaag aagaaaagag
    15541 cttcagcaag aagcagattt ttatggctct atggatggag caaataagtt tgtaaaggga
    15601 gatgccatag caggtcttat agtaacagca ataaacataa taggtggaat tgtaatagga
    15661 gctgtaatgc tgaatatgac tcttatgggt gcagcacaga catatgtaag acttactata
    15721 ggggatggtc ttgtaagcca gatacctgct ctattaatat ctacatcagc aggtataatc
    15781 gtaacaagag cttctactga tgaaaacttt ggtggattag taggtaaaca gttaacagca
    15841 ataccaaaag cagtaatgat gactggctgt gttctaatcg tacttggtat gatgcctgga
    15901 ttacctaaac cagcattttt ttcactaggt ataggtgcta tagcagcagg ttacttttta
    15961 ggaagagaaa agaaagatac tttagaagac ttggagtttg aaaaacctga tatggtaaat
    16021 ccagataaaa atgaagtaga agatgtaaca aacttaataa atgtagaatc aatagaagta
    16081 gaaattggat atggtttaat atctttagct gatgaatcag caggaggaga tttacttcag
    16141 agaatagcat caataagaag acaatgtgca atagacatgg gtatagtcgt acaacctata
    16201 agaataagag ataatttaca gttaaatcca aaccaataca atataaaaat aaaagggaat
    16261 gtagttggtg ggtatgagat aatgcctaat atggttttat gtatggatcc tatgaatcaa
    16321 ggatttagta tatttggaat aaagacaatt gagcctactt ttggaataga ggcactttgg
    16381 atagaaagtg acaaggtaga agatgcagag ctaaagggtt atacagttgt agattcatcc
    16441 acaatactta taactcactt actagatata ataaaatcaa atgcacacga attactggga
    16501 agacaagaag ttaagactat tatagatgca gcaagagaaa attatagtgc tgtagtagat
    16561 gaattgatac cagatttaat gacacttggt gaaattcaaa aagtacttaa aaatctgtta
    16621 agagaaaaag taaatataaa ggatagagtt acaatacttg agacacttgc agaccagtct
    16681 agaaatacaa aagatataga gcttcttact gaatatgtaa gaatagcttt ggctagaagt
    16741 atttgtaata atttagttga tgaagataag gcaattgtag tggctacatt gtctccagaa
    16801 actgaagaat tggtatctaa taatttacaa aggtctgtaa atggaactta tccagcagtt
    16861 gacccagaaa atacaaataa aatatttgag agtatacaag aagtaatgaa caatgtatat
    16921 ttcaatcata atataccagt aatagtagta tcaccaaaga taagagctcc atttagaaaa
    16981 ttagttgaaa ttgtatatcc taatttgact gtattatcat taaatgaaat acctaacgat
    17041 attaaaatca aagcagaagg tgtggtgagc atatatgatg acgaaaaagt atacagctaa
    17101 cacaattcaa gatgctatga atctagccaa aatggaattg ggtgacaata ttacattaat
    17161 agataaaaaa gaggtaagaa aatctggaat aagaggcatt ttttcaaaga aagatattga
    17221 attgactatt ggatgggaaa agaaagataa tttagaacaa aaagatttaa aacgagagat
    17281 agaacaacta aagtcaatta taaataatat ggggtttgat aataaaaatg ataatgatat
    17341 agataaaata tgcaagaatt tactaaactt agatttaaat caagaaattg tagaatttat
    17401 aaaagctgat ttacaagaaa tgaagttcaa tggaattgat acaagtaaaa atttagtaga
    17461 aagtctaaag aaaaagatta aaatagaaaa tcaagcaata aatggtaaaa ttgctttagt
    17521 tggaccacca ggagtaggta aaacgactac aatagcaaag ttggctgcca aattagtttt
    17581 tgaggaaaat aaaaaagtag gagtaataac tatagataca tacaggattg gtgctgtaga
    17641 acagttaaag atatatactg atataatgaa tattcctttt aaaggggtaa taagccctga
    17701 tgagatggaa ttggcacttg atgagatgaa agattgtgat gttgtattaa tagatacaac
    17761 aggtagaggc tataaaaact ctatgcaaat attagaaatc aaaaatctta tagataaagc
    17821 tgaaactgat aatatacatt tagttttgaa ctgtacaaca agagaaagtg atacaaaagc
    17881 aattatagat tcatatagaa atgtgaattt taaaagctta ataattacaa agttagatga
    17941 aacaataaca tatggctcaa tatttaacat aatgaattat gcacaaaaac caatatcata
    18001 tattacaaca ggacaaaatg ttcctgatga tattataaaa cctaatgaag ataagattat
    18061 aagattacta ttaggagttg aaagtatatg atagaccaag ctcaaatttt gagaaaaatg
    18121 gcaatggaaa aaagaggttc tgatgagatt atagtagaga atgagaatat gcctaagata
    18181 attacaatag cttctggtaa aggcggcgtt ggaaagagta atctcgctac aaatttgtct
    18241 atatgcttaa ccaaacttga taaaaaagtt cttatattag atgcagatat aggaatgtct
    18301 aatatagata taataatggg ggtaaatgta aaagggacta taattgatgt aattaatggc
    18361 gaaaaaaata tagaagatat aatttcacag actaagtatg gtgtaaatat aatatctgga
    18421 ggttctgccc ttaatcatat agaagacttt acagaagctc aaagaaataa gtttatacat
    18481 agcatagaac agattcacaa tgtggatttt ataataattg atacaggagc tggtatgagc
    18541 aaaagcctat tgtcatttat atactgtagt actgaatttt tcttaataac aactccagaa
    18601 ccgacatcat tgactgacgc atatagttta ttaaaagcta taagtaattt tggcataaag
    18661 aaaagtgcaa atataataat caatagggta attgatttag aagaagcgaa atctacatac
    18721 aaaagaataa aaatggttgt ggacaaattt ctaaacctta atctggaatt gtatggatat
    18781 ataatagatg acaaaaaagt aggtgtatgt gtaaaaaaac agataccatt cattttagaa
    18841 tatcctacaa gtattgcatc aaaatgtatt atagctatag caaagagaat ggtaagtcag
    18901 tcaagagaag atattgttga taataaaagt tttctaagaa agatgtttag tatatttaaa
    18961 agctaaggag gcgtagttaa tgaatagaga agaattaata aaagagaata tgcctcttgt
    19021 aaagagtata gcaagaaaat tttttatacc agggaaaggt tttgaatatc aagacttagt
    19081 aaatagtgga gtcattggat tgattgatgc aataaataaa tttgatactg aaaaaggagc
    19141 aaagttttct agttactctt atataaagat aaaatctgct atattagatg aaattagaaa
    19201 tcaaagtcca atttctaaac ataatctaac taaagtaaat aagtataata gagtggtaga
    19261 aaagttgcaa tctaaattat taagagagcc aacttcatat gaaattgcaa aagaattaaa
    19321 ggtttcagaa aaagaacttc atgatatcga aagtaatata gatatgttaa atattgtatc
    19381 attaaactat gtaatctttg aagatacaaa tgaaactgta caagatgtaa taagtgatag
    19441 agaagaggaa gctccagaaa atataataga agaagaagaa aagttagaga ttctatccaa
    19501 agcaattagt aatttaaatg aaagagaaaa attaatactt tcactgtact attatgaaga
    19561 tttaaatcta aaggagatag ggaaagtgct aggtgtttca gaatcaagag tttcacaact
    19621 ccacagaaaa tcaataagaa acttaagaaa caagataaaa gaacttaaat attctatata
    19681 gatggtgatt gataagatga tacaaattat attaatagta gtgtctattt taataatagt
    19741 aactatagtt ataaatttac agaaggaatc aaaaaataaa gataacagat actttgataa
    19801 aatatttgag caagaatatg aagcaaatga tagattaata attgcagaaa aaagaagatt
    19861 ctttttagac aaagtacttg cagaaaaaag aattaatagt catataaaag tttatgaaaa
    19921 ttataaccta ataaaggcta atgaagaaag aaaggaaaac atatttatag aatcaaaaaa
    19981 ggaagtaaga caagaaaatg agttatctct taaagtgaaa catcttataa gttcaggtta
    20041 tgatgatgtg gaaatatgta agatgctaga tattggaaag ggggaactgt tattaataag
    20101 gagttgttac aaaatataag gatattatta tgtgataaaa aagtattctt aggaataggt
    20161 attggtatat taatatcaac tttatgtata atgcttttta gtaataatga tatgagtaag
    20221 gcagagatag aaaaaaaggc cagagcgtta ggcatgaaat atacaagtga gatgaaagtt
    20281 ttagaataga tgatactgtt tagacattta caaaaaagga ggtcataatg tgataagagg
    20341 attatataca gcagtttctg ctatgataac taatcaacaa aagcaaaatg ttgttgtcaa
    20401 taacctagct aatatggata caaatggata taaaagtaaa caacttttga ctaaaagttt
    20461 tgatgaatta aagttggagg gttacaataa ctacgcaaat ggaactaaac aaaagcagat
    20521 tataggcgat ataagtccag gtatttctat ggatgaaact atcacaaatt ttaatcaagg
    20581 acctataaaa actactgata ataaaatgga tgtagctatt caaggtaagg gattttttct
    20641 agttagtgat gcagctggaa atcaatttta cactagaaat gggaacttca gaacagacaa
    20701 tcaaggtgac cttataacaa gtgaagggta ttatgtactt gggacgaata ctgctactgg
    20761 tgctgtaggt catataaatg taggaaatca aaaatttgaa gtggcaaaag ataatacaat
    20821 atcactagac ggaaatgcta tgtataaatt taatattgta gattttaata actacaataa
    20881 cttaaaaaaa gaaggagata acttatattc tggaggagga gctatgaaag taaataatgc
    20941 acttacaata caaagtgcga tagagtcctc taatgtagat atggtatctg aaattaataa
    21001 tatgatgaca atatcaagag aatatgaggc aaatcaaaag ataattcagg ctatggattc
    21061 taaattagca aaaatagcaa gtgagattgg ttcagtaaga taggtaatag gaggtgaatt
    21121 tttatgtaca caatgtttta tactagtaaa acagctttat cagcaaatca aagtaaacta
    21181 gatataatat ctaacaatat agcaaattct gattcaacag gatataaaaa aataaatatg
    21241 gaatattctg atttagtgga tgaagtacta aatagaccat cataccctac aaatggtaaa
    21301 gatatatcga ctggaacagg agctaaggca acagctccca tgagaaatta tgaacaaggt
    21361 gcattggctc ctacagatag taagagtaat ttggcgatag atggagaagg attctttaga
    21421 gtgataagaa aagatggtac atatgcatat acaagaaatg gtggatttaa tgtagatgct
    21481 ttaggtaaga ttgtagatga taatggaaat atattagatg tccaatttga tgcaggatat
    21541 aactataata atacaaatat agatagtaca aacctgacag tttcaagaaa tggagagcta
    21601 ttcgtagata ataaaaaaat aggaaaaatt aacttatatc aacctatagg aactcaaaat
    21661 tttatatctg aaggagatag tttatttgtc gcaactgatg cagcacagat taaacaagtt
    21721 gagaaagtcg atatagttca aggctataga gagaggtcaa atgtttcttt acaagaagaa
    21781 tttgttgact taatagccac tcaaagagca tttcaaatga actcaaaggg aataaagaca
    21841 gcagatgaaa tgtggcaaat agcgaataat ttaagagcta aataattatg taaggagagg
    21901 aatattcatg aatgatatga aagagtacga ttttggcaaa cctcagagtt attcaagaga
    21961 aaatttaact tgtttaaatt ttttattagc tgaattttgt aaaaaatata aaaactatat
    22021 aatttatgaa cttaagtgta attctagtat ggttgttgat agtatagacc aagtaaatta
    22081 tcagactttt ttagatagag taacttcttc atgcgtagta gtacaaaatg caatggaacc
    22141 tgttatgaaa aacttttctt ttagaataga tagaagtgta gctgatatgt ggattgatat
    22201 aacttctgga ggaactggag ttgtaaagga taatgataga gagcttacag aacttgataa
    22261 aaaagtgctt ttacacctta tggatgattt agtaagaaat atgcacttat ttgagggttt
    22321 tgaaaatata caagtattgg atacacatac acatattaat ttgccacaat tatgttctcc
    22381 aactgcacca gtatgcgttg tagatttaaa agtgctaaat gataatgagt gtattggaag
    22441 tgtttctctt tgttttccat acaatggttt agaggctctg ttagaaagta tatctgttat
    22501 agaatttttt gataatgatg taggagatga ttcagaggaa tttacccaaa aaatctataa
    22561 tagtgtatct aatatagaat tatcagttat tgcagagata ggaaaagtat caattaatgt
    22621 tgaggattta cttaaactag aagttggaga tgtaatagta acaaataaaa aaataaatga
    22681 ttatatagat atatttgtag aaaattcaaa atcatacaca gcaacaccag gatttatatc
    22741 aagtaaaaaa ggtgtaaaaa taaatgatgc tgttggaaaa gaggtgtagt tatggacaat
    22801 agtaatctta tttcagatga agaagcaaag atgttattaa atgataaagt tgaaagtgaa
    22861 gttgatggag atgatgagtt agccttagat gtctctgaag ttaatcgaaa taatatacaa
    22921 aggatacttg accttaagtt agaattatct gtagttatag gaagaactaa gatgactcta
    22981 agagatattt taaatttaca taaaggttct cttattgaac tggatacttt agcagaccaa
    23041 gatgtggaaa tactaataga taataaggtt ttggcttatg gaaaagtagt ggtggtagat
    23101 ttaaattttg gtgtaaagat aacaagtata gtaagtgaag aagacatggt aaaatctctt
    23161 gtttagagat aacatcaatt aatacttaag gcatgaggtg gtacttattt taaacttaaa
    23221 tgatataaaa aaagatatac taaaactcaa taaaaaagac tcatgttctc tatataatga
    23281 atctattaat aatataaata ataattgtat atcaactgga ttaaaatttt taaaacaagc
    23341 aagagattta aacccagatg atgttgatat attaaatctc acaggtcttg taaacttact
    23401 aaaatgtaat tttgatgatg ctgtagaaag tttctataag agttcacttt gtgaaaaaac
    23461 tgctctgtgt gaaaagtata taaacatgct tacatctgag gagtttttaa ttttctttga
    23521 aaagtacaat caacttataa ggttaataaa taaaggaatg tataaagaag ctatacaagg
    23581 gtttaaactc ataactgacc aatactgtga tttaatagaa ccttatgagt tgcttgcctt
    23641 actgtatgac aaggaagaag aatatataaa atttgatgaa tgtttagaag tattgaagat
    23701 tattgataag gaaaattatt tattaaatca agaaaataac acattagata tgtataatag
    23761 tgctaatata caaactaaaa gtaatataaa agtagatgat tataagaatg agttcttaca
    23821 taaaagtagt agtaaccata aaattaatga ttataatgat gtaactaaag aaaagagttc
    23881 aagcaaaaac cagaaaagtt cagattctaa taagaaaaaa agtattaaaa aaaataagta
    23941 cctatatata gccataatat taggaatatt attagtagga caaagtatat ataattctta
    24001 taagatgaat aatttaactg ataaaatatc taaaaataat gaaactaaaa atgaagagtc
    24061 aatagattct aacttgacta agaaagaaaa taatatagaa gaaaatgaag atatgagtaa
    24121 ggcttctaat ataaataaga gtaataaaaa cattaataaa gaggatgatg taaaatctaa
    24181 aaaaaatact gtagaaaatg aagatgataa agattctgac ttatttacag aggatgaatt
    24241 gatgtctaag gcgaatactt taaaatcaga aaagaaaact aaatcttcta tacaactata
    24301 taagaaagtt gcagatatag gtgaaaataa agggaataca tctgaagcaa cttatcaagt
    24361 tgctattctc agtgaaaagt taaaagacta taaaactgct gaagaatatt ataaaatgta
    24421 tgtagaaaat tactcagaaa aagatgctta ttttgatgaa tcttattata atttaggtat
    24481 gatgtactat aacaatggtg atttaaaaaa ttctaaattg acattaaaga agttggttaa
    24541 taaggtacca aatagtatgt ataataattc aaaagtaaaa gaaattttaa aagaagaata
    24601 gttaaattag ataagaaaaa taaattattt tattttaaaa taatatgatt ataatatatt
    24661 gacataccat tttaaaaaat ttaaaatatt aattataaaa tgaggaggga taaaaaatgg
    24721 aatttgaaaa agggagtatt tcaattcata cagaaaatat ttttcctata attaaaaaat
    24781 ggttatactc tgataaagac atatttataa gagagcttat aagtaatgga tgtgatgcag
    24841 taagtaaaca taaaagactt gtatcactag gagaaatatc agaaaataag tcatcagatt
    24901 ataaaattac agtatctgta aataaaggag aaggtacact taaatttatt gacaatggaa
    24961 ttggtatgac tgaagaagag ataaaaaaat acataaatca agttgctttt tcaggtgctg
    25021 aagatttctt taataaatat aaggataaaa tggaagagtc aaatgatata ataggtcatt
    25081 ttggattagg attttattct gcatttatgg tgtctaaaaa ggtacaaata gatacacttt
    25141 catatacaga aggagcgact cctgtaagat ggatatctga aggtggtaca gaatacgaaa
    25201 tatctgaatc agatgctaga aatgatagag gaactactat aacactattt attgatgatg
    25261 atagtaaaga atttttagat gaatttactg taagaggaat aataaataaa tactgttctt
    25321 tcttaccagt agaaatatat ttagaagatg ttgaaagatt agaaagagaa gctaaagaag
    25381 cagaagaaaa agctaaaaaa caaaaggaag atggaaaaga agagattgta gatgcaaaag
    25441 taattgagcc tttaaacgac actaatcctc tatggttaaa atctccaaaa gactgtactg
    25501 atgaggagta taaagagttt tatagaaagg tattcaatgt atttgatgaa cctttatttt
    25561 ggatacattt aaatgtagat tatcctttca atttaaaggg tatactttac ttccctaaat
    25621 taaaaaatga gtttgaactg actgaaggaa aagtaaaatt atacaacaat caagtgtttg
    25681 tggctgacaa tataaaagaa gttataccag aatttttact tttattaaaa ggagttatag
    25741 actgcccaga cttaccatta aatgtatcaa gaagtttctt acaaaatgac agggatgtaa
    25801 gcaaaatttc taaacatata attaaaaaag tagctgataa gttaaaatct ctttgtaaaa
    25861 atgaaagaga agaatacaat aagttttggg atgatataca aatatttatt aaatatggtt
    25921 gcttaaaaga tgaaagtttc tatgagaagg taaaagaatg tatattattc aaaactattg
    25981 atgatgagta tataacatta caagattatt tagaaaagtg taaggacaaa catgaaaata
    26041 aggtatttta tgtaagtgat aaagaacagc aatctcaata cattaaacta tttaaagaat
    26101 acgacttaag tgctgttgtt ttaaactcat caatagatac tcattttatt tcatttatgg
    26161 aatataaaga gaatggagtt aagtttaata gaatagatgc agacctttct gatgtattaa
    26221 aagataaaaa tgaaaataaa gatagtgaag aaaacaaaga agagatagct aagatagaag
    26281 gtctgtttaa agaagctgtt ggagaaagag tgaagaatta ttctgtagaa ggattgaaaa
    26341 atgaagacac tccagctatg gtgttagtat cagaacaatc tattagaatg gcagaaatgc
    26401 agtcaagatt tgctggaatg gatttaggaa tgaattttga agaagaaaaa actcttgtaa
    26461 taaatgagaa cagtccaata atcaaaaaat tggtttctct taaagatgat gaggaaaaga
    26521 aagataagat tacgcttatt tgtaaccaga tagcagattt agctttactt tctaataaag
    26581 aattaaagcc agatgagtta gattcatttg tacaaagaag taataagctt atgagtcttt
    26641 tgatagaatt ataagattga atttgaatac attgaagcaa atttataaaa agtattcaat
    26701 atcagtgata agaaagaagt gtctcaaaat gaacttttca gttcatgagg cgcttttttt
    26761 atgtgaaatt aaatttattt taatcatttc aacaatgaaa aatatattta tctctatttt
    26821 gagacagacc gttctttatt tttttatctt tggttaattt ttaaatattt aagtaggttc
    26881 taataaaaaa ctctatttag ttaaatatag ttaacttgat atataataag ataatacata
    26941 taattaagta aaagattgtt ttttagttta tgtttaaata cataattaca ctttgttaaa
    27001 aacataaaat tattactaaa gtagaaatag gaggttttag catgaatttt aattataatt
    27061 tgccagtaaa tatcttattt gggagcggca gaattaatga tgtgggaaaa gaagtatcta
    27121 aatatggcaa aaaagtattg atagtaacag gtaaaaatag tacaaagaaa actggattac
    27181 ttgataaaac aatagattta ctaaaagact ctaaaataga atatgaagtt tttgatagag
    27241 tagaacaaaa tcctcttata actactatat attcaggagt agaaattata aaatcaactg
    27301 gatgtgattg tgttttaggt cttggtggtg gaagcattat ggatgctgcc aagagtatag
    27361 ctttttctta taaaaaccca ggagatttaa atgattatat atttggaatt agacaaggtg
    27421 aagaagcttt acctataatc ttagttccaa caacatcagg tacaggtagt gaaggaaatt
    27481 gttttgcagt acttacaaat tcagaaacta aagataaaaa atctataaag aagaatagta
    27541 tgtatgcaaa agcatcaata attgacccag aacttatggt tacaatgcca aaacatataa
    27601 ttgcatcagt tggatttgat gcacttgccc ataatatgga agcatatctt tctaatggaa
    27661 gaaaccctct tgcagatgta caagctattt atgggattga acttatatca gaaaacctaa
    27721 taaaggtata taatgatgtt aatgatttag aagcatggga aaaaataaca cttgcaagta
    27781 ctattggagg aatgacgatt ggtacagcgg gtacagcctt gccacatgga atggagcatc
    27841 ctgtaagtgg tcttagagat gtaatgcatg gaaaaggact tgcagctttg actcctacta
    27901 ttatagaaag ttcatgggat agtgatattg aaagatttgg taatatttca aaggtattag
    27961 gaggaacttg tgcagaagat tgttcagatg caattagaaa tttccttaaa aaaatagatt
    28021 tagatataaa gctaagtgat ttaggtattg aaaaatcaga tatagattgg ttaactgata
    28081 attgtatgaa aatatcagtt gcaaatatta agagacatcc aaagtatttt aataaggaac
    28141 aaataaagga aatgtaccat aagtctttat aatttgactt tacatattta gttttatata
    28201 aaaattataa attaataata aaaaattata agtaattaat agtaaaaaaa ttgtgtatag
    28261 aagttatata tataataaat ttgtcttaag cttttatttt actttctaat aaataaagga
    28321 gtttaaaaaa gggaagatta attaagtaat attttataaa attcttcgat ttaaaaactt
    28381 taagtagagg tgaaataaat gtttaatccc aaaagaataa tattcgaaaa aggaacttta
    28441 gacactgatg tgggtaaaaa aatatacaat aaatttagag ataataaaga tgtagagata
    28501 attaatatat catcaaatag gattaaagaa catattccag gtgaaaatct atatgaacaa
    28561 tatagaagtg ggaagcagac tatggtagtt gggattagaa aaagtctgaa gtttcaaaca
    28621 tgtaaacctt ctgctaatta tcaactacca cttgtcagtg gatgtatggg aagatgtgag
    28681 tattgttatt tgaacacaca attaggtgat aaaccattta taagggtgtt tgtaaatgta
    28741 gatgaaatac ttgaacagac aaaaaaatat attgaagata gaaagccaca ggtaaccata
    28801 tttgaagggg cagctacatc agacccagtt ccagtagagc catatactaa ttctttgaag
    28861 aaaacaatag agttttttgg aagagaggaa aaaggaagat ttagatttgt tactaaatac
    28921 agtgatgtgg atagcttatt ggatgctgaa cataataatc atacagaaat acgatttagt
    28981 atcaatactc ctagaattat aaatgaacat gagcattata cagcctctgc aaataaaaga
    29041 atagagtcag ctgtaaaact tgctagggct ggttatcaga ttggtttcat aatagcacct
    29101 gtgtttttat atgaaggttg gaaagaagag tataaaaatc tgattataag tataaaagaa
    29161 gctttaccaa aggattttga taaaaaaatt atatttgaag ttatatctca tagatataca
    29221 actaaagcaa aaaatagaat actagagatt tatccagata cactattgcc aatggaaaat
    29281 gaagatagaa agtttaaata cggtcaattt ggatatggta agtacgtgta tgataaagaa
    29341 caattagatg atatgaaaga gttctttctt aaggaaataa atggagtatt taaaaatagt
    29401 ttaataaaat atataatata gcaaatctta cctaaagatt tgctatattt tatttttata
    29461 tattttatgt ttataataaa gcaatgagat ttagcattat catataagaa aattaaaaag
    29521 ggcactatct atcaagaaca ttttttctag ttttgatact ataaaggata agagtaacaa
    29581 aaatatcagg aggagttata gtatgcatgc atgggagtct attcaaatta cattggattt
    29641 aatagaagca aatttatcag aagaaatatc aattaatgaa ttagcaaata aagcaaattt
    29701 atcaccattt tactatcaaa gactatttaa aagattggta aataagactg taatggagta
    29761 tataaagctt cgtcgtcttg caagagcttc tgaatactta gcagaacata agaatcgtat
    29821 acttgatgta gctttaaatt ttggatttgc tagtcatgag acatttacac gttcctttaa
    29881 aaaagcatat ggattaactc cagaaaaata tagagcaaat ccagtaaaac tcaatcattt
    29941 tattaagcca gaattaattt taaattatgc tatggttgat gaaggtgttc cactgattgc
    30001 agatgatata gtgattgaag tcaatcgtaa gacattgagt aatccaagga catttgttgg
    30061 aatagaaata gaagtaccta tttgccagtt ggtgggcggt gaaacaactg gaattgcaat
    30121 agtagaggaa ttatggatga aacttggaag tcaaagacat aatataccaa atcaaattcc
    30181 aggtggaaat gaatttgctg ctctttatat gggaaatgca aaagaaggaa attgtatgta
    30241 tatggcaggt gtagaggtag agtcaggtac atcagtagaa ggatatagta cttttgagtt
    30301 gccagctaaa gaatatttag tatgtggatt tgaggcagaa aactttaatg agttagttaa
    30361 ttcagctgtt tttaaagcag ataaatttat ggaaagatgg atgaagaaac ataatttaac
    30421 aactactgat tttgcaattg agatgtatta ccccactaca cctgaagcag catatctgga
    30481 acattggatt gttccggttc ctattgaaca atagaaagga tgatgaatat gaattggcaa
    30541 gatgcgtttt ttcaagatac taaacctaca tttgagcaaa ttaatgaatt tattaataat
    30601 ccattatggg acaagttaaa taaaactttg gtttctactt ataatgtaga acctaagctt
    30661 gaatacagta aatgttcaat gcaaagaggt tggaatgtaa aatataaaaa aagaggtaaa
    30721 tcactatgta ctttatatcc acaagaaggt tattttaaag ctttggttat tgtaagtgaa
    30781 agcaatagag tagaggttga tttatttatt aatacttgtt gtgattatat caaaaaaata
    30841 tataatgagg taaacttttt taatggaagt aaatggctaa tgattcaagt agatagttta
    30901 ttggtattaa acgatatgct tgaactaatt aaatttagag cttaacatat agaacaggat
    30961 gaatatataa gtcatcctgt ttttatattc aatcagataa attaatatat agtctataat
    31021 atgaataatt tagtaaattt aaatttactt tatctttcta cattctttgc aactaagtcc
    31081 acatgtgttt ttttcttgca tattagtagc accccaatta gcaaattgtt gtaaaatagg
    31141 cacaatactt ttacctttac ttgttaatgt atattcaact tttggaggaa tttcattata
    31201 ttgtttacga tgaacaatac cagtagactc taaatctttt aatgattgag ctagcatagt
    31261 atttgtaatg ccatccaatt ttctttttat gctactatat ctaattggat tttcaactgc
    31321 taacatacat ataataggta atttccactt accaccgaca atgtctaatg cataacggat
    31381 tggacaggtt aattcttcaa cgtttttagt tatatttttt tcagtattta tactcatttt
    31441 aattactcct tagttatagt agtatctttt ttatacttta tcagaaaaaa ctgcatactt
    31501 gttttttctg tattttatat taaaattata tcaaaggata atccttaata caagtattta
    31561 aaaggggtgt aagaattatg aaagcattag aatttttgaa caaggcagga gtttattatt
    31621 tagcaacagt tgatgagaac aatcaagcac atgtgcgtcc tttaggattt gttatggaat
    31681 acaatggtaa acttacattt tgcacatcta atcaaaagga tatgtttaag caacttattt
    31741 ttaatccaaa ggttgaactt tgttgtatag accaaaacct taatactcta cgtattttag
    31801 gtgaagcagt atttgttaca tcaaaagaaa cacaagctaa ggcacttgaa ataatgccaa
    31861 gtttaggtca gatgtactct gtaggagatg gtaagtttga aatattctct attgataagg
    31921 caaaagttag ttgttgttca atgagtggta aaaagattga tgtagagctt taattataat
    31981 tgaatcgaca tccatagcga aaattaagaa aacgttatta ctgaaatatt gatagattta
    32041 atttaaatgc aataaaaaga tttttaacta atgaatatgt aaaaattttt agttattaga
    32101 tgtgtatttc aataatgata tactattttg aataattaca tactagaaat aagataatta
    32161 aaatttactt tataatggga tagatttctt ttaaaaaata ttttaactat aattgatttt
    32221 tatttaatct gaaaaaaata ttatgactat aaaaaattgc tacttattat ttggatattt
    32281 ttatatataa ataataagta gcatgacttt atacattaat aatgatagaa tataaattaa
    32341 tagataagat atgattttaa aaggagtgat ataagatgag taaatatagt tctttatggg
    32401 aatatgttaa gaaaaacaat agtcaatcgt ttaaattaac ttttgaagaa ataaaagata
    32461 ttgctgggat agagatagac cattcgtttt tgaaatataa aaaggaatta aatgaatatg
    32521 gatatcaagt aggaaaaatc tctttaaaag agaagactgt tattttcgat aaaattggtt
    32581 aagacataaa taattacata taataaaata atttgtattg atgattatta caaataaaaa
    32641 taaagtaaac cttatataag atttacttta ttttagatga ttcttttttt tagctttata
    32701 ctataaggta tataaaacta agcttattaa aaatgttata atttctattg ctatcataga
    32761 ccatatcaat ccatcaagtt cataaaagta attaagcttt attaaatagc ttctttatta
    32821 taatttcttt atttcatata atataaatag tattaaaatt tttatgggag ttaaagtaag
    32881 catgtattat tacttagata tttgtataat caacatctag taatttacct ttttgatgaa
    32941 aatgattaat aagtttttta ggataattca ttattaattc atctggaaaa ttaatttcct
    33001 ttaagatatt ttcagaatac tcatagttac ctatgtcaaa acaaatgtga gcatctgaag
    33061 ttagtatgat tttagcacca tattttttgc aaagtgtagc aatatactta cagttatccg
    33121 agctaccatt tcttgacacg cctttaatag aacaattatt tatttctatt aagatattct
    33181 tttctatagc taatttaatg attaattcat agtcgattgg gtagtttggg tttccaaggt
    33241 gtcctaatat ttcaatatta tcatgcttat ttattgcatt tattaaagct ttagtattat
    33301 tctctaatgt atttggagaa aacacagctt cgtgaaatga caagataaga taatctaacc
    33361 gaggaattac aaaaggctct aggtctatgt ttcctttaat atctagtata tttgcttcac
    33421 agcctcttaa aattataatg ttatttatga ttcttgggat attttgaatg ttgtgaaagt
    33481 accatctgtg gggagaacca ggcatcttag gaccatgttc agatgtacct agtatcttaa
    33541 ttccattttc tttacaataa ttaatatttt ctagtaaagt gctatagcca tgaccactga
    33601 ctatagagtg ggtgtgtaaa tctgataaaa aattcatata aattcctcct ctataaaaga
    33661 tgagggtaac gtaatttata ttataaaacg aataggaaag aaaatcaata gttacataat
    33721 tagacaaaaa tattaaaata ttgtagaatt aagcttaata ataaatgttt aacagtatta
    33781 ctgcttatag gggaggttaa ctatgacaca aaagaagcta tcaaggaaag aaaaagtttt
    33841 aatttgcttg aaaaggttaa cagaagaaca cttgaacttt aataatggca ttagatttgg
    33901 atttgatgca gaatatatag ctactgaagt aggtataaat agaagtaatg ctagtaagga
    33961 attaaatcaa ttaataaaag aaagtatcat tataaagata aagggtaagc ctgttcagta
    34021 cttatataaa gaaagtattg agagtatttt aggaaagaaa ctaaataaag ggatatttga
    34081 taatattaac tctataaacg aatatagtga aaatgattct atcaaagatg agtatatttt
    34141 agagagtata aaaaaagaga acaatatttt taatttagta ggatgtgatg gaagtttaag
    34201 atcccaaata gaacaggcta aagcagcagt aatatatcca cctaaggggt tgagtacatt
    34261 aataatagga cctacaggag ttggtaaatc tatatttgca gaatatatgt ataagtatag
    34321 tttaactata aaagaagata gtgataatgc accatttatc atatttaact gtgctgatta
    34381 ttctgataat cagcaattat tattagcgca gctttttgga tatgtaaaag gagcatttac
    34441 tggagctgat aaagataagt atggtattgt acatgaagct aataatggag tattgttttt
    34501 agatgaagtt catagattat ctgcagaggg acaagaaatg ctatttctgc ttatggataa
    34561 agggatatat agaaggttag gagaggctaa taaggtacac gagtgtagag ttatgataat
    34621 tgcagccact acagaagacc cagaaacagc aatgttggaa acgtttttaa gaagaatacc
    34681 tgtaactata aagattccgt ctttagagga aagaggcttt caagagagaa tgaagcttat
    34741 atgttatttt tttaaagaag agagtgttag gattggtgtt aagttaaagg tttcaaaaga
    34801 agttattaag gcatttattt tatataagtg taagggaaac ataggccaat taaaaagcga
    34861 tatacaatta atttgtgcaa gagctttttt agattatatg acctataaaa gggaatgtgt
    34921 atatgtaagg ctctctcttc taccaaataa tattagagaa tcattatatg gaaataatag
    34981 aaaagaggag tttgtacaaa atttcagttt tattggaaaa gatgatatta tttttttacc
    35041 agaagaaaag gattttgaaa gtgaaaattt attaatagaa aataaaaaat atgatttaga
    35101 tttttatgga atgataaaag aaacttggaa taaacttcaa caagaaggta ttaaagagtc
    35161 acaaattaga agtgttatag atggagacat acagaagtat tcctataatt taatgaatac
    35221 ttttatatat aatagtagta atcaaacagc ttataacaac attgttaatg attcaattgc
    35281 aactactgta aaatatattt tacaaaagca tgataaatgg agcaaaagag aaggattaga
    35341 taaattaata aaagctatag ctttacatat tcagaattta attgaaagaa taaagattgg
    35401 aaatatagta aagcatccta ataaagatga aattatgaag gaaagagcat atgaatataa
    35461 catagcaaaa gaaatattat ctaacatgtc attaatgtat ggagtagagt ttccagaaga
    35521 tgaagcaatt tttttagcaa catttcttta tttatcgtat gtgggattaa atgaggagag
    35581 tatagcaata ttagtcataa tgcatggtga atctacagca tctagcatgg tgaatgtagc
    35641 caatacttta ctggattgta accatgcagt ggcaattaat atgggattag aagatagagt
    35701 tcaggatatt ttagtacaag ctgtagaaat agccaataaa attgataaag gaaagggaat
    35761 tttaatttta actgatatgg ggtctatttt aacatttgct aaagttattc gtgatgctac
    35821 aggaaaagaa gtaaaagcaa tagatatggt aagtacacct attgtaatag aagcaactag
    35881 aaaggctcta actccagaaa tgacattaga gaaattatat tttaatatta tagaatctat
    35941 aaagaaacat tatggagata tggaagttac atatgctgaa aaagataatg gtggtagata
    36001 ttttgataga ttactaattg ataatattag taaaactttg acttttttaa atggggaaaa
    36061 ggcttatttt atattaaaag aagtaattaa tagaattagt gaacactatt ctttagtaat
    36121 tcaagatgaa cttttagtaa agtttatatt tcattgttca tgtatgattg aaagagtaat
    36181 aataaaagag tctttagttt ataaatcata tgaagaaaga attagtagaa ataaagaatt
    36241 atatttagtt ataaaagaaa gttttaagct agtagaagag acattcgata taattatttc
    36301 agatatggaa tatgcaaata tattagatat atttgaatca caatatgata cagataaatt
    36361 caaaagttga tacaaaattt aaaaatagcc aaaaggctat ctttttttta ctttttttga
    36421 tacagatttg atacaggttg aaaaaatctt ttgatacata tcgaaataat aagtttttaa
    36481 aaaaaattga ataaaaagtt ttgattaaat cctgtaaaat agccatttgt tattgataca
    36541 cataagttgg cacggcaatt gcttatataa aaagtgacaa gaaaagtgag gtgaaattgg
    36601 tgataggaat aatagttatg actcatggga gttttagtga agaaattata aaaagctgtg
    36661 aacttattgc aggaccagca gaaagaacag cagcaattaa actaaatagg aatgataata
    36721 ttgaagactt aaataaaaat tttgtagaaa agttaaatga attagatgaa ggtgacggag
    36781 ttttagtatt agctgaccta ttaggaggtt cgccaagtaa tgttgcaagt ttaaacctta
    36841 aaaaaggtgg caagtttcat gcacttactg gagtaaatct tccaatgcta cttgaagctt
    36901 taattaatag agagggaaaa agtttagaag agttagcaga agcatgtatt gaagcaggac
    36961 aaactggtat aaacaatatt aacaaagttc ttagctcaat gtaatagaaa aaggagtttg
    37021 attaaaaatg tctttagtaa aatattgcag atgtgatgat agattaatac atggtcaagt
    37081 tatatataaa tgggttaagc atctaggtgt aaaaaagata gtagtagtag atgatgaaac
    37141 aaccaatgat gttattgcta aaggtttgat aaaaatggca gcaccaaaga atattgattt
    37201 atcaatatta actgtaagtg aatcaaggag atatttttat aataatcaag ctgatgataa
    37261 cgtttttgta ttaattaaaa atttagatac tgcaaataga atgatagaag aagggattaa
    37321 tatcaaaaaa cttattgttg gaagaatacc tacaggaata ggaaaaaaga aaatatctca
    37381 aaatgtatac attaataaaa aagagtttct tttaatagat gaatttatta aaaagaatat
    37441 taatgtatca attcaaatgg tacctgatga agaagaagta gaattaaacc aaaatattaa
    37501 tcgctataaa ggagagttta tttagcaatg aataaatata aggtaatcat actgtgccat
    37561 ggaacttggg gaaaagtctt agtagaagaa acaaataaaa actttggatt gtcaaatcaa
    37621 tatgaagttt tatcactaag ttctgaaaaa gatgtttctg tatttatgaa agaggttgaa
    37681 gaagtagtag ataaagaaga tgtgaaaatt ataatatcag atttatatgg aggatctaca
    37741 tcaagtgttg caatagctgt tgctataaga agaggaatag atgcaataaa tggacttagc
    37801 ttacagacaa ttttaatcgt agatgaagaa ttaagcaaag aagataaatt acatacatta
    37861 ccagaaagaa tcgtcaaaaa aaataataac ctttgtgtgg atttaattaa agaatttaaa
    37921 aaaatataaa ttatgaggtg aatataatgg caaaaataac attagtaaga gtagaccaca
    37981 gattaattca tgggcaagtt ataacaaaat gggtaaaaat cgctcaagct caaaaaataa
    38041 taatagttga tgacttctta ggtcaagatg aatttatggc tgacatatat aaaatggcag
    38101 caccatcagg agtagaagta gttattttaa cagcagaaga tgcgggacaa gcattccaaa
    38161 acaacacttt aggagataaa aatatattta ttctatttaa aaatgttgat atggcaaata
    38221 aggcttataa agctggactt aaatatgaaa agattcaatt aggaggtatt ccaaatgaag
    38281 caggaaagaa aatggtgttt acagctgttt cattaggaaa tgaggatgta gaacaactta
    38341 atgaacttaa tgaaaatggg gttgaaatag tcctacaagt tataccagag gaatcatcta
    38401 tgacatatga aaatgcgtta aagaaattta aataaaaaat ataaattagg aggaattata
    38461 aatggaatct gttttgatgt tggcaattgt aacaggatta tggtattggt ttgcagcagg
    38521 tcttgcaggg tatacccttt tctcaacact aaagtctcct ttatttatag gttttagcct
    38581 tggattactt tggggtgatg taacaactgg tatgattgtt ggtgcaagta tagaaatggt
    38641 ttatctagga atggtcgcag cagggggaaa tattccatca gataaatgtc tagctgctct
    38701 tattgcaata ccagtagcac ttcaaactgg tgttaatgca gaagttgcag tatctattgc
    38761 ggtaccttta ggtgttatag gagtattagt taataactta agaagaactg gtaatgctgt
    38821 attggttcat aaggcagata agtatgctga agaaggtaat acaaaaggca tctggagatg
    38881 tgcaacactt tattctttaa tatttgggtt tgttttaagg tttccaatag tttttgtatg
    38941 taactttttt ggagcagatt tagttcaatc attattagat gttataccac aatggttaat
    39001 gaatggatta acagtaatgg gcggtatatt acctgcatta ggttttgcaa caacaatatt
    39061 tactataggt aaaaataaat ttttaccaat gtttattata ggatttttta tggttcaata
    39121 ttttgaaata tcaattactg cagctgcaat atttggtgtt tgtatagctc ttttaattac
    39181 ctttatgaaa gaagacaaaa gggtaggtga agtataatgc aagaaataaa aaataaagaa
    39241 gttaaaaatg gacaaggtcc aactgaagtt aataagacaa gagtacttac aaagaaagat
    39301 gtaacaaaaa catatctaag atggtggtgg acagcagaat tatccaactc ttttgaaaga
    39361 atgcaagcat tagcagtttg tgcaagtttc actccggctt tagaaaagtt atataagaga
    39421 aaagaagatt tagtagatgc gttaaagaga cacttacaat tttttaatac tcaagctata
    39481 tggggaggat taattcacgg tacagtttta gctatggaag aagagaaagc tactgaagga
    39541 aaaatcccag gagaagttat ttcaggtgtt aagaatggtc ttatgggacc tttggctgga
    39601 attggagata ctttagattt tggtacattt caaacaatat tcttagcttt aggtgcttca
    39661 tttggagcag aaggaagtgt tataggtgca tttttcccaa taatgtttag tatattacta
    39721 ttttgcgaag gttattactt atttcattta ggatattctt taggtagaga ttcaattaag
    39781 aaaattctat caggtggtat tgtcaataag ataattgatg gtgcttcaat tctaggaatg
    39841 tttatgatgg gagcattatc tgcaactaca gttaagcttt caactccgct atcatttgat
    39901 attggtggta aagcaattgt tgttcaagat acattaaata tgattgctcc aggtttatta
    39961 ccacttggtg ttgtattctt cgtatattgg ggaatgaaat acaagaagtg gacaataact
    40021 aagttattag taattttagt tgtattagca ttggtagggt cattcattgg aatattctaa
    40081 ttcacagtga ttaaaatata gaatttaatt ttaggaggag aaagaaatgt cagaaaaaat
    40141 gtatacagaa aaggaatatt tagaaggaat tagaggagca acaggagaaa gagcagtatg
    40201 gttctatctt cttatgaaag aagctgaaaa attaggagct aatccagatg atatatgtaa
    40261 agaagcaata tatggatttg gtaaaatgag aggtcaaaaa tataacgtag cagatactcc
    40321 aggaaaaatg gcagaaatgt tatataacag taaaggacaa aaagtgtttg aaatggaatt
    40381 agtagaaaat acagatgaga atggcgtttt aaaattccat cactgtccat tagatgctgc
    40441 ttggaaagaa tatggattaa ctaaagaaga aagaaaagaa atttgtagat tagcttgtta
    40501 tggggattat ggaagagttg actgcgcaca aggtgttaaa ttagattttg ctcaaaaatg
    40561 tgctcatgat gatgaagttt gtgaattagt atttacaaaa aaataatttt taatgctaaa
    40621 agcctgttct ttaaggacag gctttaaaaa gatagaaagt aggttgattt atggatataa
    40681 gcagaagtgt agataatagt ttaaatagaa ccatagaatt tctaaaagaa ttgataaaaa
    40741 ttgattcaca acaaggtgaa cctatttcac aatgtccttt tggtatagga cctaaaaaat
    40801 ctttagataa aactttagat tactgtgcta gtttaggttt tagtgttaaa aatattgata
    40861 attatattgg atatgcagaa attggagagg gagaggaatt aatagggatt ccaatgcatt
    40921 tagatatagt tccaccagga gaggggtgga gtgtagaccc attttcggga gcagtaattg
    40981 acaatattat ttatggaaga ggtgtaatag ataataaggg tgctgtatct atgcttatac
    41041 atgtattgaa aaacatagag gatatgtatc ctacaattaa taaaagaata agattaattt
    41101 ttggtacaaa tgaggagaca ggtatgaaat gtatcaaata ttacttagat aaaggtgaag
    41161 aaattccaag tatgggattt actccagatg ctatgtatcc agtggttaat ggagaaaaag
    41221 gaagggttca tataaggata gaaaaagaaa ttaaaattga caaaagtaaa ccttacataa
    41281 tagtaagggg aggaactaaa gaaaatgttg ttccatctca ttgtactgca aaaattatta
    41341 atggaattat ttcagagtta actactaaag gagtagctgt acatgcaagt aatcctgaaa
    41401 agggagaaaa tgctatatct aaaatggtaa taaagatagt tgaggataat atggattttc
    41461 agcatagaga agatatcgaa ttagtttcaa aatacctttg tagtgattat tatggagatg
    41521 cattagggat taatcaatat gatgaggtat tcaaaaacac taccttaaat ttaggaatat
    41581 taaaagtaaa tgaggaaaaa atcgtatgtg agttagatat aaggtatggt aaaaatatag
    41641 ttttaaacaa tataatagat agattcaaaa aggttttttg taatggttgg aaaatagagg
    41701 ttattgctca taaagactta cattatgtgg atgagagtaa tttagtactt aaaaaattat
    41761 tagaagccta tgaagaagta acagatgaaa atggatatac aattgctatg ggtggtggca
    41821 catatgcaag ttggtttaaa gatatggtgg cgtttggacc taagttctta gcgtataaaa
    41881 ctggtggtca tggagtagat gaaagagtac caataaatca tatacgaaag aatatggaga
    41941 tatatacttt ggctttaata aaactattag aattgtaaaa gaagaagaga ctttatgtgc
    42001 aaatttacag atatcttaaa aagagagata aaacgagttc aaccttattt aaatgttcaa
    42061 caccatcaaa tggggctgtc tcaaaataga gataagcctc ttacttattg ttgaaatgat
    42121 gaaaatatat tttacttcat acaaaaaaga gtgccttatg aagtaaaaag ttaattttga
    42181 gacaccctca tttgatgatg ttgaagagaa aataagtatg aaattattaa aaaagatgat
    42241 tttataaaag ttaaggttga aaagaaaagt taattgaatg gaattagata aatggagaag
    42301 gatgtatact atctaataaa ataacaaaag agtgttataa agttggttat atgattagag
    42361 aaaagtctat tgatacttat ccagatagtg gatggggatt tatgacttgg gatgaaaatg
    42421 gagaataata aaaattatag tttatacagt ttaataagat atatgtacaa gcagttaatc
    42481 taaatgattg actgcttttc ttttatagat ttttatataa tagacaaata ccaatacatg
    42541 atagttgcct gttataatta gagaaataaa tacgaataat aactttataa aagaattaaa
    42601 tggaaaatcg aagagttata ttcagtattt taaaaagaat atgaaatgag aaatgaaaat
    42661 aaggtgttta tattaaaaga ggttttacac aaaatattta aaaacgtgat aaataaaaac
    42721 tgtatttaga tagattaact attagtttta tataatatgg attttattta caaagtttac
    42781 atattttaca aaagagatta aagataaatt taaatatata ttttatatac ttgtttattt
    42841 gtttctctgt ggttgatttt ttcgtcaaaa gtcaacttca aatcgcttgt tagctttaca
    42901 tgtttaagga tataattata cataaggagg gattgacatg aatgaattaa acatcgcaaa
    42961 aacgctgatt ttaaagcgta aagaaaaagg aattacacaa gatgaacttg caaattatat
    43021 aggcgtttct aaagcttctg tatctaaatg ggaaactgga caaagctatc ctgacataac
    43081 ttttttaccg caactcgcga catatttcaa cattaccgtt gatgagttaa tatgttatga
    43141 acctcaaatg atgaaggaag atattaataa actgtataat aaactgtgca aagactttac
    43201 ggttaaacca tttgatgaag tcatggatga aataagagaa atcataaaaa aatattattc
    43261 atgttttcct ttgatttttc ggatgggtct tttaatagtt aatcactatg atattgtaga
    43321 tgaaaaaaag cgtgaactat taattagtga agcattagaa atatttattc gtatacagga
    43381 aacttgtaat gacatagata tttgccgtca agcaaaaagt atggaggcaa cttgttatat
    43441 attgttaaat caacccatac aagtgattga tttattgcaa aacagcaatt ttcccatgat
    43501 aaatgaatct atacttcttg cacaggggca aatgatgaat ggtcaaatgg atgaagcgag
    43561 agaaacattt caacttggtg catatcagaa ccttatatca ttagtccaaa acctagtggg
    43621 tattttgcaa aatgcagata aattacaaat gaaggaaata gaaagacgta ttctagcaat
    43681 ttcagatata tttgagcttg atacattatc tcctgctatt atgctttctt catatttaac
    43741 acaggcacaa attaatttaa tacatggtga taatgaagga gctattaaat cattacgaaa
    43801 atatgtagat ttagcaacac gtgatatata cccaattata atacatgggg atgacttttt
    43861 taataaactt gacagatgga tttctgaaat tggaacaggt attacacgtg atgatacaat
    43921 cgtaaaagca ggtattgttg ctgctattaa aaataatcca atgttttctg tattaagtga
    43981 aaacaaagaa tacaaatttc ttatagaaaa attatcttta ttggaggaat aagattatgg
    44041 aaaatttgat ggaatactta ccacttttgg ttcctgttat tattttagat ttaatactta
    44101 taattactgc acttgttcat gtattgagac atccaaatta taaaattgga aataaggcaa
    44161 tatggattat tgtagtactg tttattagtc taataggacc aattttgtat tttacaatag
    44221 gaagaggtga agaatgatga atattgtctc aataaatgga ttatcaaaag gttttggtaa
    44281 tcgaaagata atagataatt taaattttac tgtaccagaa gggtcagtct ttgggtttgt
    44341 aggtaagaat ggtgcaggta agactacaac aatgaaaatg gtacttgggt tacttaaacc
    44401 agatagtgga actatagatg tttgtggtga gaaagtgacc tatgggaaaa caagttctaa
    44461 tcgtcatgta ggttatctgc ctgatgttcc agagttttac aactatatga gaccacttga
    44521 atatctttct ctttgtggtg aaattacggg actttcaaaa aaagaaatac agataaggag
    44581 tgaagagttg ttatcacttg tgggtcttgg aaatgaaaag agacgaatag gtgggttttc
    44641 tcgtggaatg aagcaacgac ttggtatagc tcaagctttg ctttcacgcc ccaaattact
    44701 tatttgtgat gaaccaacaa gtgcacttga ccctgttggt cgaaaagaaa tattagatat
    44761 tatgcttaaa atcaaagact caacaactgt aatattttca acacatatac tatcagatgt
    44821 tgaaagaatc tgtgaccatg ttgctatatt aaacaaagga agcattgccc ttagtggtac
    44881 actttcagaa ataaaaagta tgcacggaaa ggatagactt ttactggagt ttgcaagtaa
    44941 tgatgaaatt caaaagttta agtctagtga tggtattaaa tccttactaa aagattcaga
    45001 agaaactaat atggaaattg ttttgcatgg taaagatata aaagcaatac aaaaaacagt
    45061 tatatctaca ctcgcagaaa tgaatctttg tccagtaaag atggaactaa tagaattatc
    45121 acttgaaaat ttatttttgg aggtagtaaa gtgagagaat acatagcatt tacaaaaaaa
    45181 gaatttaaag agaatttaag aaattataaa ttatttagtt taattatttt atttctaata
    45241 tttggtataa gtagtcctat ttctgcaaaa tttatgccag acttgattgc tcattttgca
    45301 ccgactttaa aagttactgc tgcaccaact gctttagatt cttggacaca attttttagc
    45361 aatatctctg gtcttggtat gagtttaact ctaattattt tttgtaatat tttatcaaat
    45421 gaatattcta aagggacact tgttattatg ctgactaaag gtttatcacg ttcatctata
    45481 gtattgtcta aattctctgt tacggtgatt attatgacaa ttggtttttg gttgagtttt
    45541 ttatttgctt atggctatac aatatatttt tggccaactg caaatcttaa tcatattata
    45601 ttttcagcat ttaatttatg gcttatagga attatgtata ttagtgtatt aattcttggg
    45661 tgtgtactat ttagaccagc atttgccagt gttttacttg tattggtggc aactctttta
    45721 ttgagtttaa tttctattcc taaacagatt gcaccttata ctcctaattt cttgatgtca
    45781 aaaaatatag acttgatttc agggaaagtg gcaatgccag aatttattat tccaattatt
    45841 ataacagttc ttatttctgt agtatgttta ctagcagctg ttattttatt taataaaaaa
    45901 ccagtttaat aataccttta tcacaaggta aagtaatctt atgttgttaa aaactacacc
    45961 taaaaatcaa aaagacttgg acttaagttt caagtctttt tgatttttaa tatatagaat
    46021 taagtgtaga aaataataca aataatatta tttgtatcaa ataaatataa gaatatttaa
    46081 ttatgttaaa atatactaaa aattacttta aaagtgataa aattagtata agtaaatatt
    46141 ttaaaggggt gtagatatgg aaaagtcaaa agtatatttt tgtgatttat actcaaattc
    46201 gcaaaataaa aacgtaccaa acaatgtaag aagattattt gatgaagcag gattcaaaga
    46261 tttaatagaa aagaatgacc aagttgcaat taaacttcac tttggagaaa agggaaatac
    46321 tacatatatg agtcctgtgg cagttagaca agtggttgat aaggttaaag actgcgaagg
    46381 aaagccattt ttaacagata caaatacact gtatacaggg agtagaacaa attctgtaga
    46441 ccatcttaca actgctatag aaaatggatt tgcttatgca gttgtaaatg caccagtaat
    46501 aattgcagat ggattatata gtagaaatta tgagaatgta aaaatagaca aaaaacattt
    46561 tgaaagtgta aaaataggtg gagaaattta taattcttct gcaatgatag taatgagtca
    46621 ttttaaaggt catgaagcag caggttttgg aggagcatta aaaaacttag ctatgggatg
    46681 tgcaagtgca gcaggaaaac aaatgcaaca ttctgatgtt actccagtag taaaggaaaa
    46741 gaagtgtgta ggttgtggaa agtgtgttaa ttcttgtcct acaaaagcta taagtatagt
    46801 ggataaaaaa gcagttatag attctgatgt atgttatgga tgtggagaat gtccaactgt
    46861 ctgtccaact agagcagtaa ctatacaatg ggaaagtgac tcagatgtgt ttgtcgaaaa
    46921 gatggcagaa tacgcttatg gagcagtatc aaataaaaaa gacaaggtag ggtatataac
    46981 ttttgttatg aatgtaactc cactttgtga ttgtgtacct tggtcaggta gaccaattgc
    47041 ccatgatata ggaatattag catcaacaga ccctgtagct attgaacaag catgctatga
    47101 tttgatttgt aaggaaatgg gtcatgatgt tttcaaacat gaacatcctc atgtaaatgg
    47161 aactagaata atagactatg catgtgaaat gggtatggga agtaaagaat atgaattaat
    47221 aaaattataa gaatagtagc acttaaatgc atataattat tgtaagagaa atctaaaagt
    47281 atcaaaggag tatgcattta tgaaaattta ctatgagaag gaaaacagca agaaggctaa
    47341 tgtaaacaat attccaagtg tatttgcgtt taattttcta agaaactttt tgactagcaa
    47401 agagtatgag gaaactagaa aagaatattt tataaataat ctaacaaaaa gtcaggttaa
    47461 tcaagccatg aaggatttaa aatggctatt tagagaatat gaaggcttag aggttattac
    47521 aatggaaagc ttggatggag ttaagacaag gatagtttta taaaccagta agaattatat
//
LOCUS       DAYAOX010000003        46223 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.003, whole genome shotgun sequence.
ACCESSION   DAYAOX010000003 DAYAOX010000000
VERSION     DAYAOX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46223
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.003"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            29..2677
                     /gene="polA"
                     /locus_tag="ACGI57_000114"
     CDS             29..2677
                     /gene="polA"
                     /locus_tag="ACGI57_000114"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFG9715206.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            2686..3288
                     /gene="coaE"
                     /locus_tag="ACGI57_000115"
     CDS             2686..3288
                     /gene="coaE"
                     /locus_tag="ACGI57_000115"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFG9715207.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            3281..3835
                     /locus_tag="ACGI57_000116"
     CDS             3281..3835
                     /locus_tag="ACGI57_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFG9715208.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            3835..5550
                     /locus_tag="ACGI57_000117"
     CDS             3835..5550
                     /locus_tag="ACGI57_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9715209.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKVKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            5623..5835
                     /locus_tag="ACGI57_000118"
     CDS             5623..5835
                     /locus_tag="ACGI57_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFG9715210.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            6036..6635
                     /locus_tag="ACGI57_000119"
     CDS             6036..6635
                     /locus_tag="ACGI57_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715211.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            6863..7234
                     /locus_tag="ACGI57_000120"
     CDS             6863..7234
                     /locus_tag="ACGI57_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9715212.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            7366..8640
                     /locus_tag="ACGI57_000121"
     CDS             7366..8640
                     /locus_tag="ACGI57_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFG9715213.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            8751..9695
                     /locus_tag="ACGI57_000122"
     CDS             8751..9695
                     /locus_tag="ACGI57_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715214.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            9911..11248
                     /gene="rsxC"
                     /locus_tag="ACGI57_000123"
     CDS             9911..11248
                     /gene="rsxC"
                     /locus_tag="ACGI57_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFG9715215.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            11271..12248
                     /locus_tag="ACGI57_000124"
     CDS             11271..12248
                     /locus_tag="ACGI57_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HFG9715216.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            12248..12817
                     /locus_tag="ACGI57_000125"
     CDS             12248..12817
                     /locus_tag="ACGI57_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HFG9715217.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            12830..13423
                     /locus_tag="ACGI57_000126"
     CDS             12830..13423
                     /locus_tag="ACGI57_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit E"
                     /protein_id="HFG9715218.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            13438..14013
                     /gene="rsxA"
                     /locus_tag="ACGI57_000127"
     CDS             13438..14013
                     /gene="rsxA"
                     /locus_tag="ACGI57_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFG9715219.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            14029..15000
                     /locus_tag="ACGI57_000128"
     CDS             14029..15000
                     /locus_tag="ACGI57_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFG9715220.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            15220..15804
                     /locus_tag="ACGI57_000129"
     CDS             15220..15804
                     /locus_tag="ACGI57_000129"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFG9715221.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            15849..16508
                     /gene="radC"
                     /locus_tag="ACGI57_000130"
     CDS             15849..16508
                     /gene="radC"
                     /locus_tag="ACGI57_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFG9715222.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            16578..17639
                     /locus_tag="ACGI57_000131"
     CDS             16578..17639
                     /locus_tag="ACGI57_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HFG9715223.1"
                     /translation="MAKEKKKEKKGFFSFNKMTKDMGIDLGTANTLVYIKGQGIVVRE
                     PSVVAIRDDSKEVLAVGEEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQK
                     AADKKGVVSPRIAICVPFGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVE
                     EPEGNMVVDIGGGTSEIAVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERT
                     AENVKINIGSTFKDDEEINMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIK
                     STLERTPPELASDIMENGIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTG
                     KSVEDQEIFEKVLMMNTKN"
     gene            17649..18542
                     /gene="mreC"
                     /locus_tag="ACGI57_000132"
     CDS             17649..18542
                     /gene="mreC"
                     /locus_tag="ACGI57_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFG9715224.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            18557..19045
                     /gene="mreD"
                     /locus_tag="ACGI57_000133"
     CDS             18557..19045
                     /gene="mreD"
                     /locus_tag="ACGI57_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFG9715225.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            19057..22035
                     /locus_tag="ACGI57_000134"
     CDS             19057..22035
                     /locus_tag="ACGI57_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFG9715226.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            22133..22816
                     /gene="minC"
                     /locus_tag="ACGI57_000135"
     CDS             22133..22816
                     /gene="minC"
                     /locus_tag="ACGI57_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFG9715227.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            22840..23637
                     /gene="minD"
                     /locus_tag="ACGI57_000136"
     CDS             22840..23637
                     /gene="minD"
                     /locus_tag="ACGI57_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFG9715228.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            23655..23939
                     /gene="minE"
                     /locus_tag="ACGI57_000137"
     CDS             23655..23939
                     /gene="minE"
                     /locus_tag="ACGI57_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFG9715229.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            24024..25154
                     /gene="rodA"
                     /locus_tag="ACGI57_000138"
     CDS             24024..25154
                     /gene="rodA"
                     /locus_tag="ACGI57_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HFG9715230.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            25195..25608
                     /locus_tag="ACGI57_000139"
     CDS             25195..25608
                     /locus_tag="ACGI57_000139"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFG9715231.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            26093..26422
                     /locus_tag="ACGI57_000140"
     CDS             26093..26422
                     /locus_tag="ACGI57_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFG9715232.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            26426..27217
                     /locus_tag="ACGI57_000141"
     CDS             26426..27217
                     /locus_tag="ACGI57_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFG9715233.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFVFGLLCIIAYFILRFKISLIKNNN"
     gene            27347..28167
                     /locus_tag="ACGI57_000142"
                     /pseudo
     CDS             27347..28167
                     /locus_tag="ACGI57_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428476.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(28379..29572)
                     /locus_tag="ACGI57_000143"
     CDS             complement(28379..29572)
                     /locus_tag="ACGI57_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HFG9715234.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            29891..31735
                     /locus_tag="ACGI57_000144"
     CDS             29891..31735
                     /locus_tag="ACGI57_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HFG9715235.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            31737..32438
                     /locus_tag="ACGI57_000145"
     CDS             31737..32438
                     /locus_tag="ACGI57_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HFG9715236.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGAESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            32456..33877
                     /locus_tag="ACGI57_000146"
     CDS             32456..33877
                     /locus_tag="ACGI57_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFG9715237.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            33978..34289
                     /gene="rplU"
                     /locus_tag="ACGI57_000147"
     CDS             33978..34289
                     /gene="rplU"
                     /locus_tag="ACGI57_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFG9715238.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            34304..34621
                     /locus_tag="ACGI57_000148"
     CDS             34304..34621
                     /locus_tag="ACGI57_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFG9715239.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            34635..34925
                     /gene="rpmA"
                     /locus_tag="ACGI57_000149"
     CDS             34635..34925
                     /gene="rpmA"
                     /locus_tag="ACGI57_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFG9715240.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            35036..36313
                     /gene="obgE"
                     /locus_tag="ACGI57_000150"
     CDS             35036..36313
                     /gene="obgE"
                     /locus_tag="ACGI57_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFG9715241.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            36365..36733
                     /locus_tag="ACGI57_000151"
     CDS             36365..36733
                     /locus_tag="ACGI57_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HFG9715242.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     regulatory      36825..36931
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            37034..38239
                     /locus_tag="ACGI57_000152"
     CDS             37034..38239
                     /locus_tag="ACGI57_000152"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFG9715243.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     gene            complement(38440..39006)
                     /locus_tag="ACGI57_000153"
     CDS             complement(38440..39006)
                     /locus_tag="ACGI57_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9715244.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(39286..40545)
                     /locus_tag="ACGI57_000154"
     CDS             complement(39286..40545)
                     /locus_tag="ACGI57_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HFG9715245.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            40670..41383
                     /locus_tag="ACGI57_000155"
     CDS             40670..41383
                     /locus_tag="ACGI57_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFG9715246.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            41501..42775
                     /gene="larA"
                     /locus_tag="ACGI57_000156"
     CDS             41501..42775
                     /gene="larA"
                     /locus_tag="ACGI57_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HFG9715247.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            42792..43580
                     /locus_tag="ACGI57_000157"
     CDS             42792..43580
                     /locus_tag="ACGI57_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFG9715248.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            43594..44814
                     /locus_tag="ACGI57_000158"
     CDS             43594..44814
                     /locus_tag="ACGI57_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFG9715249.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            44825..46216
                     /locus_tag="ACGI57_000159"
     CDS             44825..46216
                     /locus_tag="ACGI57_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFG9715250.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
BASE COUNT        18256 a         5068 c         8750 g        14149 t
ORIGIN      
        1 aatagataat ttttaggagt gatataattt ggaaaaaaaa ttaattataa tagatggaaa
       61 ttcaataata aatagagctt tttatgctct tccagaaatg aataataaag aagggctaaa
      121 gaccaatgca atatatggat ttacaacaat gctatttaaa atgatagata tatataaacc
      181 aacacatata agtgtagcat ttgacagaaa agcacctaca tttagacatt tagaatataa
      241 agagtataag gctggaagaa agggaatgcc agatgaactg gcagaacaat tacagccatt
      301 aaaagacctt ttagataaat ttagaataaa tagattggaa atagatggct atgaggcaga
      361 tgatattata ggtactgttt ctaaaaaagc agaagaaaat ggatataagg tatacatagt
      421 aactggagac aaagatgcta ttcaacttgc ttcagacaat acaactactc ttataacaaa
      481 aaaaggagtt ggagaagttg aagaatacaa ctttaattct gtaattgaaa aatacgagat
      541 gactccaaca caatttatcg atttaaaggg tcttatggga gacaagtctg ataatatacc
      601 tggagtacct ggaataggtg aaaaaacagg tataaagctt ataaaagagt tttctagtat
      661 agagaatcta atagagaata cagataagct taaaggtagt gtaaaaaaga aaatagagga
      721 aaacaaagaa atagctattc aaagtaaaag acttgcaaca attataagag atgtgccaat
      781 agaagtggat ttagatagta tgatttttgg cgattatgat aaagatgaac ttttagataa
      841 atttagatac tttggattta caagtctctt atcaagatta gttgatttgg tcgattcaga
      901 tgacacagaa cacgtagaag aaaaaataga aatattaaaa ttaaaagata tagaaaaatt
      961 tatagatgaa gtaaacaaaa aaggacaagt tattttaaaa actgttagag gacaaggtaa
     1021 tatacttgaa aaaaatataa tatatatttt tataagtgta gatggagaaa aaatatatta
     1081 tgtagattca aatgaagtgt caaagttaga tagtatattt tcaaataatg aaattaaaaa
     1141 gtatggatat aatttaaaag atgattatat atctttaaaa ccatataata taaacttaga
     1201 aaatatgtat tttgatataa caatagctga gtacttaata gattcaactt catctacctc
     1261 atatgattgt agtgcaatag ctatgaagta tttatcaaaa aaggttaaat caaaagaaga
     1321 attattagga aagggtgtta aatctaaaaa ctatgaggat ttagaatttg aggatttagc
     1381 agagtatatg ggtcaaataa tatgcacagt taaagaaaca gttcctatga tggaaagaag
     1441 tctaatggat atgagtatgg atggtctttt ttatcatgta gagatgcctt tagtagaagt
     1501 cttaggtcat atggagtatg aaggtataaa agtagacaaa agtatattgg atgaattgag
     1561 tgttgaattt aaagaaataa tagctacatt agaaaaggaa atttatgaat tatcaggaga
     1621 gccattcaat ataaattcac caaaacaatt gggtgttata ttatttgaaa aattagagct
     1681 tccaataatt aaaaaaacta aaactggata ttcaactaat gcagatgttt tagaaaaatt
     1741 aagagataag catccaataa tagataagat aactgagtat agacaaatag taaagttaaa
     1801 ttctacttac gttgaaggac ttttaaatat aataaatcca gaaagtaata gaatacattc
     1861 atcatttaat caaactataa cgactacagg aagaatttca tctacagaac caaatcttca
     1921 aaatatacct ataaaaatgg aaatgggtag aaaaataaga aaagtgttta ttgcagatga
     1981 taactgtaag ttggttgatg ccgattactc acaagtagag ctaagagttc ttgcacatat
     2041 gagtcaggat gaaaatatga tagaagcatt taaacatcat gaagatattc ataccaaaac
     2101 agcttcacaa gtttttaatg ttcctatgga agatgttact agtgaattga gaggtgcagc
     2161 taaagctgta aactttggta ttatatatgg taaaagtgac tttggtctag ctgatgattt
     2221 aaatatacct gttccaaagg cgaaagaata tatcgaaagt tattttgcta aataccataa
     2281 aataaaagag tttatggaca caacagtaga aaaggctagt gaggatggat atgctgtaac
     2341 aattttaaac agaagaagat atataccaga aataaagtca agcaatttta tggaaagaaa
     2401 tagaggaaag agatttgcaa tgaatgcacc aatacaagga agtgcagcag atataataaa
     2461 aattgctatg ataaatgttc ataaaaaatt ggaagagaac aatttgaaat caaaacttat
     2521 actacaagtt cacgacgagt tgatagttga agcaattgat gatgaaatag atatagttaa
     2581 gaaaatagta aaagaagaaa tggaaaatgc tgtaaatatg gatgttcact tagatgtaga
     2641 tttaaatgta ggtagttctt ggtatgagac aaagtaggtg attttatgtt gatattagga
     2701 cttacaggag gaataggctg tggaaaaagc agcctatcaa atatatttag aaatcttaac
     2761 attcctatag tggatgctga tattatatcc aggaagatat ttgaggataa gttgttattg
     2821 gaaaaagtat ttgtccattt tgggcaaagt ataaaaaatg atgatggaac tctaaataga
     2881 aaagctcttg ggaaaatagt ttttagtgat gaagaaaaac ttaaggaatt aaataacttg
     2941 actcatccac gaataagaga aaaaataata agtgaaatag aaaaattgag aaaaaaaggc
     3001 gaaaatatag tagtgttaga tgcggcaata ttggtagaaa gtggtttttt agatatggtt
     3061 gacaagctat tagtagttac ttgtaaacaa gaagtacaaa ttagtagaat acaaaagaga
     3121 gataattgca gtgagcagga agcacttagt aggataaatt cacagatgag tcaggaagaa
     3181 aaatccaaat atggtgatta tataatagat aattcgggaa caataactga attagaaagt
     3241 aaagcacata aatttattga gtacatgaag gagaattggc gtgaatagta agaaagtatt
     3301 gattctatct atttttataa tcttatttgg ggcactatta atggaaagca aagtaataca
     3361 taaattttta tatcctaaaa aatattcaga gtatgtagaa aagtattcga aagaatttaa
     3421 tttagatgaa aatatagttt acagtgttat taaagccgaa agtaagttta atagttctgc
     3481 tgtttcaaaa aaggaagcaa aaggattaat gcaaatatta gacataacta gagattgggg
     3541 agcagaggaa ctaaatttaa aaaatgtgga tattttcgac ccagagacta atataagact
     3601 tggctgttgg tatttaagta agttatacaa agaatttggt aaattagatt tagtgatagc
     3661 tgcatataat ggtggttcag gtaatgtgaa aaaatggtta gaaaataatg aatatagtaa
     3721 agatggcgaa aatctacatg atataccttt taagcaaact tcaaaatatg tagaaaaagt
     3781 aaaaaataat tacgaacatt ataataagat atatggcaag aaaggaaaaa actaatgaag
     3841 agaataaaag tcttaacagt cgttttagct ataactctta tggtagctgg ttgtagtaat
     3901 actaaaacaa aacaaagtag tagtgattca agtttacaaa gtcaaagttc tatagatgct
     3961 aagtatataa acttaacaat ggtaactcca aaaactataa atcctataac taatactgat
     4021 aagtctgttg gttatataat gaatttagtt tatgatagtt tatttactat agatgagaac
     4081 tacaatgtaa ttcctcaatt agttaaggag tataatatag ctcaggatgg aatgagtata
     4141 gatataaagc taaaagatgc aaagtggcat gatggaaaaa atgttacttc aaatgatgta
     4201 aactatacta ttggtttaat tcaaaaaagt gcagatagtc catataatga atttactaaa
     4261 aatatagctt ctgtgaatat taaatcagat aaagatttta caataaaatt taaagctaga
     4321 tatgcttttt caattgatag tttaattttt ccaattgtat ctcaaaatca tttagattca
     4381 aaagatgtaa atgacaagaa taataatatg ataggaaatg ggaaatataa aatagaatca
     4441 tatacagaaa gagaaggaat ggttttatct gtaaacaaag attactatga agaagttcca
     4501 aaaaccatga agaacataaa agtaggtatg gtgcctaatg aagatgcaag gacatctatg
     4561 gttatggctc ttgatagtga tataaccaat gttacattaa atgatttaag taagtttcaa
     4621 gaaaaagagt ttaatataac aaaatatcaa ggaagagatt atgagtgtgt attatttaat
     4681 tataataatc cttttttcaa agatgttaat tttagaaaag caattgcaca ctctatagat
     4741 aaagatagaa ttattagtga aggttatatg gatgatgcta ctccagttaa ttttccactt
     4801 aattcaaaat caaaatacta taatagtgaa atgaaggatt tagagtttaa taaagataag
     4861 gcgatagagt gtcttgctaa ggtagaatat gcaaatgtaa actcagtaaa tcaaaatgac
     4921 aataaagtaa aaaatcagaa aaaatcagct aaaaagaact caaagaagct tacgccagaa
     4981 gaagaagcaa aagttaaaaa agcagaagaa gatagaatca aaaaagaagc tgaagagaaa
     5041 aaaaatagag aaaaagagga agtaaagaaa agtctttctg aaatgaactt aaagataata
     5101 gtaaacagag ataatagtga gagaataaaa actgcaaaca taataaatga aaacttaaaa
     5161 gctataggaa taaatagtac agtaaatcag ctctcagata aggatatgga aaatgctctt
     5221 aattctaaaa attatgattt agcattagtt ggatggaaac tatcaattat accagatgca
     5281 agtagtatta tagcgagtag tggatatact gatgataagt taaatggata tatgtctgca
     5341 cttacaagtt ctacgtctga aattgaaaca aaaaaagcat acaaagatgt acagacatat
     5401 ataaaagata atgcaacatt tataagttta gctgtgagaa acaactatat tgtaagtaat
     5461 agtagattaa aaggtaaaat tactccaaat gattttgatg tatatgaggg aatatctaat
     5521 ttagatataa aatcgaatga atcaaattaa atgattaatt tctatttaaa aagtataaac
     5581 tatatgatag aataccctta tagggtataa ggaggtaatt aaatgaagaa gaaactatta
     5641 gtagaaggta tgagttgtgg tcattgtgta aaccatctta aaactgcatt aacagaagat
     5701 atagatggta ttgaagtttt agatgtagat ttagaaaata agtgtgcatc tgtagatatg
     5761 aaagatgatg tatctattga aaaattaaaa gaagtgatag cagaattagg atttgaacta
     5821 aagggaattg aatagaaatt gataaactaa aaaaagacct ctattggggg agtaatagag
     5881 gtctaaaaat gggggagatt gagtatgttt gattttacat tactattatc tcccctttta
     5941 aattaatata tacataaaaa gtaaaaaaat tttgtaatga ctcacatttc ttgagctttt
     6001 tgaatatact ataaatagct aagggaggga gtatcatgga atctagggga tataaagagg
     6061 atttaaaaaa aatgatactt gatatggccc acaaagaact tgagagttat gtatcacaaa
     6121 aaggagcaaa aaaatacttt gaaggtaact tggataagat tattaataat aatataaaaa
     6181 aattcaatca aagtattggt cttaatagag tatatttaaa aatgttaaaa aagtgtgcat
     6241 atcaagaaag tgtaacttca atatcaagaa ctataaagaa agaagcatta tttaaatcaa
     6301 aaaaagagct atttgctttt tcagagtact taaaacttga tattaacaag cgactttcat
     6361 ataatcaaat tttaagaaaa atatcaagat atatatacta caatagaagt tcatatgctc
     6421 aaaaatacgt tgttttcaaa agaggagatg aagaatatct tttagaacca gagaaaataa
     6481 aagatgaatt aatatcaagc tataggtcaa agactagaga agatatgagg tcaatagcaa
     6541 aacttttgga tatagaagta gaagatgatt ataatgctga agatattaga aaaaaagtaa
     6601 taaattatat tattaaagaa aaacttaaaa gttagagttt taaagaataa gttctgccaa
     6661 tttgcatgat taagtataac ttgggtataa attttttatg catttgaaag agcttattct
     6721 ttttttgaca tattctttta aataatttac catgatttgg taattggtca tcatcattca
     6781 aatttttaac ttaatatgat ttaaataaat ttataataat gatataatat aaaaaaaata
     6841 aaaattggag agtgatttca aaatgaagtt taatttttgt cataacaatt tcaatgttac
     6901 taatttagaa aaaagtttag atttttataa aaaagcttta gggcttaaag aggtaaagcg
     6961 caaggaagca gaagatggaa gttttatatt agtttattta ggagatggca ttactagtca
     7021 tacattagaa ttaacttggc ttagagattg ggatagacca tataatttag gagacaatga
     7081 atttcatctg gctttagaag ttgaggagtt tgatgaggca aaaaaacttc ataaagattt
     7141 agattgcata tgttttgaaa atgaaagtat gggaatttat tttatagcgg acccagataa
     7201 ttattggatt gaaatacttc ctaaaaatca ctaattttaa ggatatgttt ataatttctt
     7261 tacaattaat aagaaaataa gttatcatta aattgtaggg actaaaaatg gataataatg
     7321 ttatgaattt atgactataa aaatttgttg gggggagtga tagtagtgaa aaaagctata
     7381 gcagctttag gaattggggc agtagcagta tctgttagct ccataaatgc aagtgctctt
     7441 gaaaagggaa cagtaacagc aagtgccttg aatataagaa gtggaccaag ttctgattgc
     7501 gataaggtag caaaattata caagggaaaa actgtagaga ttttagaaaa gtctaatgga
     7561 tggtataaag taagggtatc gagttctgtt gttggatggg gaagtgcaaa atacatatca
     7621 acaagtggtt cgtctgaagg tacatcaagt caaaataatc caacttcaag tgggacaact
     7681 attagtggaa atggtaaagt aaatgtgagt tctagactga atgtaagaag tggtgcaggt
     7741 actaactatt cattagttgg taaagcaaat aatggagatg tagtaaagtt actagaacaa
     7801 agcaatggat ggtataaaat aaaactatca aatggagtta ctggatgggc tagtagccaa
     7861 tatatttcta aaacatcaga agatgttgga acgaacaatt ctagtaattc aaatagtact
     7921 aataattctg ataaaaaacc atctagcgaa gaatcaattg aaggcaagaa tggtaaggta
     7981 acatctgctg tgagtttaaa tgtaagaagt ggtcctggga caagctattc tataatagga
     8041 aaattaaatg gtggagatgt tgttgaatta aaagctaaaa gtaatggttg gtataaggta
     8101 aagttatcga gtggaacaat tggatgggtg agtgccagtt atatttctga gactaatgag
     8161 gatacaaaag aaaaaccaaa ttcatcatct aaccaaaatt ctcaatctaa tagtaactca
     8221 aagccatcat ttactggaaa ttcagataaa tctacagcaa aaggttcaac aatcgtagat
     8281 tttgcatata ctcttattgg aataccatat caatggggag cttcaggacc tgataaattt
     8341 gactgttctg gatttacaca gtatgttttt aaacattctg taggcgtttc tattcctaga
     8401 gtttctagag aacaggctaa ttttggaagt gcaatttcta tgggaaatta tgcaccagga
     8461 gatttagttt attttgacac tgatggtgat ggaacaacta atcatgtagg tatatacgta
     8521 ggaaatagta aatttatcca ttgtagtggt actcagacta atcctaataa ggttaaagtg
     8581 gataatttaa ctagttcata ttggtctaaa gtattacttg gtgcaagaag atttgtttag
     8641 taagtacaat ttcttattaa aaaattagaa gaaataatta tttatattca taaattgtga
     8701 ctcttaaaaa tatactttat taataatata attttgggag ggacaaacta atgaatagaa
     8761 gaaaaacgat atacagagga tttaataata atagacataa gattgattta cgtaaatatg
     8821 ttataacgat tgcttgcttg tgtctaattg gctattattc ttatacaaaa ataaaagata
     8881 gcaagatatt ggagtatgta tcggctaaaa taccatttct aaataattca tcagatataa
     8941 cttataaaga tatatctgat gaattaaatt caataaaaaa agggaaaaaa tctaaatcac
     9001 aaaccaattc agatgataaa caagaaacta atccagaaaa agcggtaaat aacactaaag
     9061 agccagaaga agtcaaatta gctactatag aaggctggga tatgtatact attcaagttg
     9121 ctgcaataga taataatgat gatttgaaaa aaatacagac atcattagtt aataatgaca
     9181 tacctttttc agttatggag aaggatggag taaaaaaaat acagacctat tcctcttttg
     9241 atgaaaatga tgttagaaag cagataagca gtgtaagaaa ggtgtttcca gatgcatttt
     9301 tatcacactt agatgcacct atgttgtctt tagagtatac aagtaactac gcttatatag
     9361 agagtatatc taaagaatta aataaattaa taacaaactt taaagaagaa tcaagttttt
     9421 ggtcgaatgc tgaaaataat gtagatatgg aaaaatataa tactattttg acaaatagaa
     9481 aggcaatatc acaaaatata tcaaaagaag ctgaaaagat tgattattct gaaatgcgtt
     9541 tatttaaaga taatttaata gagtatgtaa agaatgtgaa tgaaaaaatt gatacagctt
     9601 caaaagcagc aaatgaagaa aaatatagtg taagtaaaag tctgctttta tcatccatgc
     9661 aggagtactc tatgtttata aattctataa aataaaatac agaaaatttg gactttagaa
     9721 agctatatga aaaggattgt ttaattaagc aatccttttc ctcaatttga cttaatatag
     9781 aggtgttgat ttataaaata aattataaaa atacggatat ttttttaaca attgttaaga
     9841 ttttttaata tatatagtat acttatagaa ggcagaaaaa atgttttttt atagataagg
     9901 gggtaaaaca atgaaactct taacttttaa gggagggata catcctccat atagaaaaga
     9961 gtactctaac acaaaggcac ttgaaaaagc tcaagctcca aagatagttt acattcctct
    10021 tcagcaacat ataggggcac cagctaaacc tattgttgaa gttggtgatg aagtaaagtt
    10081 tgggcaaaaa ataggtgaac aacaaggttt tgtttcatgt aacgttcatt cttcagtatc
    10141 aggtaaagtt attgctattg aacaacatga agtaccagga ggttcagctc aatgtgtagt
    10201 tatcgaaaat gactttaagg aagaattaca tgagagtgtt caaccaaaag gtcagttaga
    10261 ggatttgagc aaagaagata tagtaggtat cataaaagag gcaggaatag taggtatggg
    10321 tggagctaca ttccctaacc atgtaaaagt atctcctcca ccagatagca aggcagaagt
    10381 agttatatta aatggtgcag aatgtgaacc atacttaact gcagaccaca gattaatggt
    10441 tgaaaatcca gaagatgttg tgtttggttt aagagcatta atgaaggtat tagatgtcaa
    10501 aaaaggattt ataggaattg aaacgaataa accagatgct atagaagcaa ttcaaaatgt
    10561 agctaaagac tatagcgaaa tcgaagttgt tggccttcaa gtgaaatatc cacaaggagc
    10621 agagaaacaa cttatttatg cgtgtacagg caaagaagtt ccatctggtg gattaccaat
    10681 agcagcaggt gcagttgttg acaacgttgc cacagcagca cagatagcta agtctattaa
    10741 aacaggtatg cctttggttg aaagaatcac tacaataaca ggtagttgca tcaaagagcc
    10801 aaagaaccta ataacaaaag ttggaacatt agtttcagaa ataatagaac aatgtggtgg
    10861 atttaaagaa gataagaaga ttggtaaggt tataatgggt ggacctatga tgggaatagc
    10921 tcaatatact actgaaatag cgactaacaa aggttcttca ggaattttat gtttggatga
    10981 ggaagaatca cgtactccag atattcaaaa ctgtttaaga tgtggaagat gtacagatgt
    11041 atgtccatca ttcttacaac cacttttcat aagtgcatat tctttaaaag atgactatga
    11101 cacagctgaa tatcatagag caatggattg tatagaatgt gggtcttgct catttatttg
    11161 tccagcaaga agaccattac ttcaatctat aagatcagca aagagagaaa taggagcaaa
    11221 gagaagaaag caagctgctc agaaataaag aaagttaagg gggaaaaacg atggaaaata
    11281 agttgatagt atcatcttct cctcatgtga gaagtaatga agatacttca tatataatga
    11341 aacaagttat tatagcactc cttccagcag cagtagcagg agtatacttc tttagactta
    11401 atgctttgag tgctatgttt ttttgtatac ttggtacagt aggtactgaa tttttatatc
    11461 aaaaatttac gaagcaaaaa agtactatag gagatttttc agctgttgta acaggattat
    11521 tattagcatt taacgtacca gcatcacttc cttggtggat gtgtctagta ggaggaatat
    11581 ttgcaatatt agtagttaaa atggtatttg gtggaattgg atgtaacttt gtcaatccag
    11641 cacttgctgc aagagcattt ttattagcat cattcccagt agcaatgact gcttggactc
    11701 aaccaggtgt taactggata ggtaaaaatt tagatgcagt tactacagca actccattaa
    11761 gctttttgaa aaatggagca gcaggactgg cagacctttc tagcaatgga attagtcttg
    11821 ctgacatgat gatagggaat attggtggat gtataggtga gacatcagca atattattat
    11881 tattaggtgg agtataccta atgtataaag gcataataaa ttatgttata ccagtatttt
    11941 acatagcaac tgtatttata ttaacattcc ttttaggtgg atttaatata actttcgcaa
    12001 tatatcaact gtttgctgga ggacttatgt taggtggatt cttcatgctt acggattata
    12061 caacttcgcc tatgactaaa aaaggtcaaa taatatacgc tgtattagct ggtcttataa
    12121 caactgttat aagaatgtat ggtggatatc cagaaggtgt atcttactca atactattag
    12181 tgaactgtct tgctccactt atagataagt ttgttagaaa tagagtattt ggggaggtgg
    12241 ctaaataatg aacagtatgg tgagattagg tggtacttta cttgctataa gtgcaatagc
    12301 tgcattagct cttggagcta ctaatcaggt tacagctcca gtaatagaac aaagaaatat
    12361 acaagcaaat aatgaactta gaaaagcagt ccttccagaa gcaaaagaat ttaaagaaat
    12421 gaaggaagat acttacaaag gccttggaga tggtcttata gctgaagtgt atgaaggttt
    12481 agatggttct gaagtggttg gatatacttt aaaagctaaa ccaagtggat atggtggaga
    12541 aattgaagtt atggtaggta tatcatctga aggtcaagtt acaggtgttg acattggtaa
    12601 tatgtcagaa acagcaggtc ttggagcaaa agctaaggat gatgcattta agggtcaata
    12661 caaaggaaaa actgctgagc ctcttgaagt agcaaaagga agtacaacag cagataatca
    12721 aatattagca atatcaggtg caactattac atctacagca gttactacag gagtaaatgc
    12781 tgctatagat gttttcaata gtgctcttaa taaataagga gggttaatta tgaatttagc
    12841 taaagtattt aaaaatgggc taatagatga gaacccaaca tttgttcaag ttataggtat
    12901 gtgtccaaca ctagccgtta caacttcagc aataaatgga attggtatgg gtctttcaac
    12961 agcagcagtt ttaatatgtg caaatttagt tatatcttta ataagaaaaa ttacaccaga
    13021 taagataaga atacctatat ttatagtagt tatagctaca tttgttacaa tagtaggtat
    13081 gcttttaaaa gcatatgttc ctgctcttga taaggctctt ggtatatata taccattaat
    13141 agttgttaac tgtttaatat tagctcgtgc agaaagtttt gctttcaaaa caggagctat
    13201 gccttcaata gttgatggtg taggtcaagg tcttggattt actgttgcac ttacaataat
    13261 aggtgcagta agagaattac ttggaaatgg tagtttattt ggaatgacat tatttggagc
    13321 atctttccaa ccagtattaa tatttatatt accaccaggt gcattcttaa cattaggatt
    13381 cttatttgca ggatttaata aattaagaag taaaaaagca tagtggaggt gtaataaatg
    13441 aatttaatac ttttattttt aagtatcgtt cttgttaata acgttataac atctcaattc
    13501 ttgggtatat gtcctttctt gggagtttct aagaaggttg atacagcagt agggatggga
    13561 gttgcagtta cgttcgtttt aactctagct tcaattataa cttattttat acagatttta
    13621 ttagtaaaaa ctggaactgg attcttacaa acaatagctt ttatattggt tatagcttct
    13681 atagttcagt tcgttgaaat ggtaattcaa aagatgagtc catctttata tcaagcatta
    13741 ggagtttatt tacctcttat aactacaaac tgtgcagttc ttggtatagc tctagttaat
    13801 gttcaaaaaa gttataattt agtagaaaca ataataaacg gatttggagc cggagctgga
    13861 tttactttag caatagtaat attcgcaggt ataagagaaa gactagaatt agctgatata
    13921 ccagaagcat ttaaaggttt ccctataacg cttatatcag caggtttaat gtcaatagct
    13981 ttcttaggat ttacaggact tataaagcta taaaaaagag gggtgaaaat ggtgatactt
    14041 acagctgtat tagttttagg aattatgggc ttaatcttcg ggatagtact agattttgca
    14101 tctaaaaagt ttgctgtaga agtagacgag agagtggaag ctatactagg agtacttcca
    14161 ggagccaact gtggaggttg tggatttcca ggttgtggag gtcttgcaaa tgcgatagtt
    14221 gaaggcaatg caccagttaa tggttgccct gtaggtggag cagatgttgg tgcaaaagtt
    14281 ggagaaataa tgggaataag tgctgaagca ggtgagaaac aagtagcaaa agttatttgt
    14341 aaaggaactt gttcaagtgc aaaagataaa tatgagtatg aaggaatatc agattgtaga
    14401 gcagcaaatg ttttaaactc aggtgctaag atgtgtaaat ttggatgttt aggtcttgga
    14461 acatgtaaag atgcttgtaa atttgatgct atttctatag ttgatggaat agcagtaata
    14521 gatgaagaaa agtgtgtaaa ttgtggaaaa tgtaaagaag tttgtcctaa gggtataata
    14581 atcacaaaac cagaaagtca agaagtagtt gtagagtgta atagcaaaga gtttggtaaa
    14641 gctgttaaag aaaaatgtac tgctggatgt attggctgtg gaatgtgtgt aaaagcttgt
    14701 aaatttgatg caataatatt tgaagacaaa atagctaaga tagaccctaa taagtgtgta
    14761 ggttgtatgc aatgtgtagc aaaatgccct acaaaagtaa tatcaggtga cataactaag
    14821 aaaaagaaag ttactattga ccaagaactt tgtgttggat gtacagtgtg caaaaaacaa
    14881 tgtaagtttg atgcaataga gggagaatta aaagaaaaac ataaggtaga tgcagataaa
    14941 tgtgtaggat gccatttatg tatggaaaaa tgtccaaaga aagctattaa aattttgtaa
    15001 tatggacaaa ataaggatat ggaataattt ccatatctct aatgagtaca taaaacagag
    15061 gtatggaata atttccatac ctctatttta tgtatattta ttctaatggt aataaatata
    15121 acaatagtgt aacaaaaaag gttcgacatt tctgttgaat tttttacaat atagtgatag
    15181 actaaatagg tacaaattac gtacaaatga ggtgaataaa tgaatataat cttggcatca
    15241 gcctctccaa gaaggaaaga aattctagag aatacgaatg taagatttga tataattaaa
    15301 aatgaaattg atgagattat attggaaggt gaaacaccaa aacatttagt tatgagatta
    15361 gcttttgaaa aaagtatgtc tgtagcatct gaacataatg aagatgttgt tattggagct
    15421 gatacagttg ttgttttaga caatgctatt ttaggaaaac caaaagatga gtcttgtgca
    15481 agagatatgt taaaaagact atctggaaga gagcatcaag tcataacagg tattagctta
    15541 ataaacttgt gcgaagataa aaaggtaatt gactatgtaa tcagcaatgt gaaatttaaa
    15601 acgttatcag agcaggatat tgaggattat ctgaaaacta atgagtcatt tgacaaagca
    15661 ggagcctatg gtatacaagg ttatggagct ttgttagttg aagaaattag aggagattat
    15721 tttaatatag ttggtcttcc aatttcaagg ttaggcgacc tattaaaaaa atattttagt
    15781 ataaatcttt tttatggagt gtgatttatc tgaaaaagtc atttaattca accattaagg
    15841 taaaagaaat ggcaccagag gaaagaccca gagaaaagat gcttgccaaa ggcgtaaaaa
    15901 gcttatcaaa tgctgagttg ttggctatac ttttaagaac tggcaacaag aataaaaatg
    15961 ctattgaatt agccaactat ataataaata gagatattca gggtattaga catttagaag
    16021 atatgacaat agaggagctg tgtaatatag atggaattgg gttatcaaaa tcaacccaaa
    16081 ttaaagcagc tttagaacta ggctctagag tagctagttt taaacctata aagtataaaa
    16141 taaggaatcc ttgggatata cagaggtatt atatggatag cctaaggtat ttaaaaaaag
    16201 aggtttttaa agcggttctt ttaaacacaa aaaatgagat aatatctgat gtagatgtat
    16261 ctatagggac tttaagctcg tcattagtcc atccaagaga ggtttttaaa gaagctataa
    16321 gaagaagtgc aagtaagata atagtgatgc ataatcaccc ttcaggaagt gtagaaccat
    16381 caagagaaga taaaaatatc acatcaagac ttattaaatg tggagaaata attggaatag
    16441 aaataataga ccatataatt attggagatg gattatactt tagctttaaa gaaaatatga
    16501 taatttgact ttatatgtga taaaataata gaagaatgtt ttaaacatga ttcttaatag
    16561 taggaaggag taaaatcatg gctaaggaaa aaaagaaaga aaaaaaaggt ttttttagtt
    16621 ttaataagat gacaaaggat atgggaatag atttagggac tgcaaataca ttagtttaca
    16681 taaaaggaca aggtattgtt gttagagaac catcagttgt cgcaataaga gatgatagta
    16741 aagaggtatt agcagtaggt gaagaagcca aaaaaatgat aggtagaaca ccaggaaata
    16801 tagtagctat aagacctatg aaagatggag taatagctga ttttgacata actcaatcaa
    16861 tgattagcta ttttatacaa aaagcggctg ataaaaaagg tgttgtaagt ccaagaatag
    16921 ctatatgtgt tccttttgga gttacagaag tagaaaagag agctatagaa gaggctgcaa
    16981 gacaagcagg agctaaagat gcattcctta tagaagagcc aatggcagcg gctatagggg
    17041 ctggattaaa agttgaagaa ccagaaggta atatggtagt tgatattggt ggaggtactt
    17101 cagaaatagc tgtaatatct ttaggaggaa tcgttactgc taaatctata agaataggtg
    17161 gagacgaatt tgacgagtct atagtggctt atgtaaaaaa agaatataat cttatgatag
    17221 gtgaaagaac tgctgaaaat gtaaaaatta acataggttc tacatttaaa gatgatgaag
    17281 aaataaatat gcaaataaga ggtagagatt taatctcagg attacctaaa acaatagaaa
    17341 tatgctctac agaagttaga gaagcattaa aagaacctgt aagttcaata gtagatgcaa
    17401 taaaatctac actagaaaga actccaccag aattagcctc agatataatg gaaaacggaa
    17461 taatgctaac aggaggaggc gctttgctta gaggtcttga taagcttatt acacaggaaa
    17521 ctggtatgtc tgtacaaata gcagaaacac ctcttgattg tgttgcactt ggaacaggta
    17581 aatctgttga agaccaagaa atctttgaaa aagtattaat gatgaatact aagaattaga
    17641 agttggtgat ggccttgaga tttgacaaga acaaaaatga gaaaaaaagg attaatgtta
    17701 aagtgatagc aacgggcgta gttgctatca ctttaattgg aattgtgggt atatcaattg
    17761 gtaaattttc tagtggtagt ccagtaaatt taggagtagc ttcagatgct attacaagtg
    17821 taggtaaggg aataaatgat ggattttctt ttatcaaaaa tggttttaaa gatgtagcaa
    17881 attttaaaga taattctaaa aaggtaaaaa aattagaaga agaaaatgaa aaacttaaaa
    17941 aaaatatgat agctttaaat gccaaactag ataaaacaga atcgctagaa gagttaaaaa
    18001 agacactgaa ctttgtacaa gaagagtata aggcgacaag tatttcagca agtgttgttg
    18061 gtaaaaatga tggaaactgg tatgagagtt ttgtcatagg tgcaggaaaa aattcaggtg
    18121 taaaaaaaga aagtatagtt atgaatggta gtggattagt tggcatagtt tatgaagttt
    18181 ccaacaatta tagtaaggca atttcactat tggattctaa ggcatctgta agctttaaac
    18241 tagcaaagga tgccaatgct aaaggtacaa ttactcaaaa tactacttta gataataaag
    18301 atagttataa tagtaaagga tatttgcaag gatatatgtt tgactcatca tataatgtaa
    18361 ttcaaggaga tataataaca acatcaggtc ttggattctt cccagatggt ataccaattg
    18421 gcgaagttga aaaagtggtt gatgataaag ataagtcatt aaaatatgta gttgtaaaac
    18481 cttatgttga ttttaagaat ataaatgacg ttgtagtcat agaaccaaga aatataggat
    18541 aaaggggata aaaacaatga aaaaagtttt actttgtctg ttggggattg tgcttatgac
    18601 tatagaaaat agtataacaa attatataga tatttttggc ataagcttta atttagttct
    18661 tatatatgta accataatct ctctttattt agatgaatta gaaggtggga ttatagcagc
    18721 aataataggg cttattaaag acataaccgt tggtggaatt tttggtgtaa atgcattaat
    18781 tttgtttatt attgcatatg caattggata catgagagat cagctatata aaggtagtta
    18841 tattattact ttcgttttaa tttttatagg cagtttaatt gattcaattg tcaatatagg
    18901 tacatctagt ataatttatc aaagctacaa catatcaaca ctttttgtaa aaggtctact
    18961 agtagcacca atagtgaata gtttaattgg tttagtaata tatcgcttat ctaaaagagc
    19021 agttttaaaa cttaaagaag attagttggt gatttcatgg aagaaaataa acaagttgat
    19081 agactaaaaa taattaagat agtcatgggc gtgatatttt tagctatttt agtaaaaata
    19141 atctatatga ctacatttaa atatgaatat tacaatgagt tagctgagaa taaaacatat
    19201 aaaaagttgg caatagaagc tccaagagga gaaataaaag atagatatgg aagactgttg
    19261 gcaggaaata aaaacttatt tactgttcaa gtttcaggta acgatattaa taagaaagat
    19321 gcaaataagc attcaagagc aaatgaaata tcattaaaac ttataaattt gctggagaga
    19381 aatggtgaag aatatgtaga tgagtttcct atatatgtag aaaatggaaa gtactattat
    19441 acatatgaca gagatattag ggaatataaa tcagaaaatg gtattccaaa tgattataat
    19501 gctaaggaaa gtttttatta cttggtggac aaacttatat cagctggaat attatctcaa
    19561 gaagataaaa gattggatgc gacaagatta caggcaaaac taaatgaaaa tggatattat
    19621 ccacctattt tagttagtaa atggatgttc acagcagaaa gagataagag agattggttg
    19681 gctagttata agataaaaga aactaaactg agtgccaaag aagcgtttga aaaagtaaga
    19741 aatagtgatg cattagaaat tgataaaagt ttgagtgatg aagatgcaag aaaaataatg
    19801 gtcgtaaggg atttgataaa atcaaaagga tattctcaat ataatccagt aactattgct
    19861 aaggatgtag gagaaactac gattgctcaa attgaagaaa gtgctatgga ccttgttggt
    19921 gtttcaatag cagtagaacc agtaagatat tatcctaatg ggtcattagc ttctcatatg
    19981 ttaggatatg taggaaaaat gccatccact caaatagaat cataccttca aaaaggttat
    20041 gaaactggag atatggtagg tcttgcagga gtagaaaaga gtaatgaaag tagattaaga
    20101 ggaactgatg gttataaaat ggttaaagtt gatgcattgg gtagaatcag taaagaaatt
    20161 gaatctaaaa aacctaaatc aggagatact gtgtatctta cactagataa agatttacaa
    20221 gaagtttcag acaatgcatt aaaacagata atcgaagttg cttctaaagg aggtactttt
    20281 aaaagtaaat ttggggacaa accaattagt gcatatgcag gaaaagcaca gtcagcagct
    20341 cttatagcta ttgatgttaa aaatggagaa gtattagctt catcaagtta tcctaattat
    20401 gacccaaata aatttgcaaa aggtatttct acagaggatt ataaagcact tcaaccaaaa
    20461 aatccaaatg acttactagc aggaagtcca cttttaaact tggttacaca aggggaattt
    20521 cagccaggtt cttcatttaa gatggtaact tcaatggcag cgttagaaaa tggtctggat
    20581 ccaaacttta ctataaatga ccctggggtt ataatgttag gtaaaaaatc ttttggtgac
    20641 tatgtatgga accatggtag aggaaaccat ggtatgacaa acttatataa agcaatacaa
    20701 gagtcttgta atatatatat ggcaacaatt ggtacaggta aaacatggcc agatggaaaa
    20761 agcattggta tagatatgaa tgcaaataaa atacttgagt atgcaaaatt atttgggctt
    20821 gaccaaaata cagggcttca agatgaagta gaagaaagag caggtaaagt tcctagcaca
    20881 gaagataaat tgaaatcaac acaagctctt ctaaagtcaa atttagaaag agaaatggca
    20941 aatgattttg ttgatataac tagagaaaaa aatcctaaag aatatgagaa aagaatcaat
    21001 gagattgtaa gctgggcagc agaaaagaaa actcctggta gagttgaaac aatgaatagg
    21061 ctaaaaaaat tggatgttaa agaagataga attgaagaag tagcagattt agcagtattt
    21121 agttatttta actttgcaaa atggagtact gcagatacat ttaacttagc tataggtcaa
    21181 ggtgaaaatg cttatacacc agctcaaata tcaagatatg tagctgctat agcaaatgga
    21241 ggtaatttag tagaattatc tgtagttgat agagctgttt caagtgatta ttcatcagtt
    21301 aaaattaatg accagaagaa agtcgaaaaa atacctttta aaaatccaga taacttaaaa
    21361 gaattaacaa aaggtatgaa attggttgca agacaaggta cagcgaagag tgcttttgct
    21421 gattttccaa tagatgtagc agcaaaaact ggtacagctg agaaaagtgg gaaaatacct
    21481 acagataacg agtatgaata tttaaaaagt catatgtcat cttataatgt caacctaaat
    21541 gatgcgatta aactagctga taagatgaaa gcagaaaaag aaaaagagct ttcattagct
    21601 aaggaaaagg aaataaaaaa gaaattagaa aataaagatt tgaaagacga agaaagaaag
    21661 aaattagagg aagaattaga agatggagta aaagttagat tagaggacac tgataaggtg
    21721 aattcttcat atctaagaaa agctataaaa gaattaaatc caaaaataac agatgaccaa
    21781 atagatagat ttaaacaaga ttatggttct tttacgtgga ctgttgcttt tgctccagca
    21841 gatgacccag aaatagctgt tgtatgtgta attcctcaag gtgattcaag tgtattctca
    21901 cttttaccaa ctagagaagt tattggaact tatatgggat tagagccaac taatagtaaa
    21961 aatgacaaca agacgaatga tgttaacaat tcttctgacg aaaatataaa tttcgagtca
    22021 caaattaata gatagctcaa tctatgtata aactttagct ttatatagaa aaaataaaag
    22081 ggtttaaagc gtatttgaag aatataaatg atatatcact ctggaggttg ttatgtcttt
    22141 aagagaaatt tgtagtcaag aactagtaga gttcaagggt aataaaagag ggataatagt
    22201 aaatataaaa agagaagctc catttgagga aatacaggaa aaaataataa acaaattgga
    22261 agcatatgtt ggctttttta atggggctaa aattagtaaa attaatagtg attgtttaac
    22321 tgacatggag attctagaac ttaaagaagg aattacttct agatttgatg tggaatttgt
    22381 tgaagatcaa aaaattgaag aaaatagtaa ttttccaaca aaatatgtca ataccctaag
    22441 atctggtgaa aatattgaat ttgaaggtga tgtagttata ttgaatgata tgaaacctgg
    22501 ttcaaaagtc ctttcaaaat caaatacagt tgttatggga gatataaatg ctggtgctaa
    22561 ggttgtagca ggaggtaatg tttttgtaat gggtaaaata gaaggttttg tacatgcagg
    22621 tgcagaaggc aatgaatttg catatgttgt tgctggaaat cttaatccta aaatattaca
    22681 aatagcagat aatattgcag aagctccaga tgatgaagaa aattatgaaa gtgaatcaga
    22741 aattagccca gaaatagcct ttgtaagtaa tggaagaatt gtaatcgaaa gctatttgtc
    22801 aaagttagac aaatagaaag aaaaataggg ggtagtatta tgagcgaagt tatagttata
    22861 acatctggta aaggtggggt tggaaaaact actaccgcag caaacttagg aactgcgcta
    22921 agcctagaaa ataaaaaaac agtagttgtg gatgcagata ttggacttag aaacttagat
    22981 gtagtaatgg gtcttgaaaa tcgaatagtt tatgacatag tagatgtagt tgaaggaact
    23041 tgtaggctta agcaagctct aataaaagac aaaagatttg ataatctata tttattacca
    23101 gcagcacaaa ctagagataa aaatgctgtc tcagttgagc agatgattga cctgtgtgag
    23161 aaactaaaag aatctttcga gtatataata atagattgtc cagcaggtat tgagcaaggc
    23221 tttaaaaatg cagtagctgg ggcagataga gctattgtag ttactaatcc agaaatatca
    23281 gcagtaagag atgctgatag aataataggt ctattagaag caaatgaaat aaaagagata
    23341 agattagtta taaatagaat tagaaatgat atggttaagc gtggagacat gatggataaa
    23401 caagatataa tagaaatatt agcaatagat ttgttaggtc ttgttcctga tgatgaaagc
    23461 ataattatat caacaaataa aggagagcca gctatacttg attctaagtc acttgctggt
    23521 caagcataca aaaatatcgc aaaaaggata ctaaatgaag aagttcctct acttgatctt
    23581 gaagttgaag atggattctt tggtagactt aaaaaaatgt ttagcatggc taagtagcaa
    23641 aggaggatac atctgtgtta gatttattta gagttttttc taacgaggct aaaactagta
    23701 aatctgttgc taaagagagg ttaaagctag ttttagttca tgatagagta gattgttcac
    23761 cacaactttt agagatgata aaaacagaca ttttgaaagt aatagctaat tatgcagaaa
    23821 tagaagatga tggtcttgaa ataaaaatgt ctaaatgcag aggtgagcat gatgacaaac
    23881 ctgtatctgc attagttgct aatataccac taaagaatat aaaagataga tgtatgtaat
    23941 tagtgcttaa aagggggata tccttattgg tatatgcccc ttttatttaa ttatatgttt
    24001 aaagatagaa agacggtgat tctttgaaaa aaatcaatat taattataag tcgacaatta
    24061 agttgataaa acagttagac tggaaactta tagttacagt attagctatc tttatttttg
    24121 ggcttgttat acttagtagt gcaacacatg ctaattcaac aggttcatat aatcaattga
    24181 ttaaacaagg attagctttt gtacttggta taggaatgat tatagtcata ttattttttg
    24241 actataacct tctaggcagg tattataaag cgctttatat aataagtctt attttgttag
    24301 ccatagtttt attacctgga attgggacag taaaaggtgg agctaggtcg tggattaact
    24361 taggaccact tgacttgcag acatctgaaa tagtcaaatt aacatttgta ctaagttatg
    24421 caaagatact tgaatccaaa aaggataaat taaatacgtt aaaggaagtt atgcctgttg
    24481 tagtttattc tcttccattt atcggattat tgattgcaca accagacttg ggaacaggaa
    24541 ttgtattttg ttgtatgata tttgcaatgc tgttcacagc aggtcttagt tcaaagttga
    24601 taaaaagagg aataatcata ctattagtgt caatgccact gatgtattta atgatggcag
    24661 accatcaaaa ggtaagaata gaagcttttc taaatccaga agatgtaact ctaaaaggaa
    24721 actatcaagt tatgcaatct ctgattgcaa ttggttctgg aggcgtaact ggtaaaggac
    24781 tttacaatgg aagtcaaaat caagaagatt ttttacctgt acaagatagt gattttatat
    24841 ttgctgttgt aggtgaagaa cttggtgtaa taggaatggc agtgttgata atcttattta
    24901 tgatattcct attaaggctt ttagcaattg caagagatgc aaaagatttt tatggaaccc
    24961 tcatagtggt tggtgttatg ggaatgtttg gttaccagat aatccaaaat ataggtatga
    25021 cggttgcgtt aataccagta actggagtaa cattaccttt tgtaagttat ggaggaagtt
    25081 cgttattgac atctttagca aacttaggat tagtactaaa tgtgtgtatg agaagaaaga
    25141 aaataaattt ctaaatttaa atacatctaa acataaattg aggagattag atatatgaat
    25201 atagcattag tagcacatga ccaaatgaaa aacactatgg tcggattttg tataggttat
    25261 gaatcaattt taaaaaagta tggactatat gcaactggaa ctacaggaaa aagaataatg
    25321 gatgaaactg aattaaatat aaatagatta gcatctggtc ctttaggtgg agaccagcaa
    25381 ataggttctt taatagtaac acaagaaata gatttagtta tttttttaag agacccattg
    25441 acatctcagg ctcatgaaac agatatacaa gcgttaataa gactttgtga tgtttatcat
    25501 gttccaatag ctacaaatct agcttcagca gaaatattta taaaagctct tgatagagga
    25561 gaactatcat ggagagaagt tagaaaaagc aaaagtcaac gtatttaaaa aatattaata
    25621 ttacagtcta aagaaacaga tatataagtg tttaatgccc tagtttttct gttggaataa
    25681 atctagtacc tgtagaaatc ttatgaaaga tttaaatgat gaagagctaa atttgagatt
    25741 aaataattag gaaatagatt atagatagag tagtagaatt taaactatta ctctatttta
    25801 ataatatgaa atagaatcaa gtctattatt atatatattt aaacttatag gtaatactat
    25861 ttcaaccaag taaatgttgt tatgacaaca ttaataaaac aaaaatttat ttttaaaatt
    25921 tgataaatag ctgtatatta gtatttatag attaaatatt aatatacagc tatttattat
    25981 ttttaagtct attgtagaat ataaaccttg tattatagag tactatgtaa tattatagta
    26041 aatataagta ctatacatta tagagtagta ggtgaaaagg cggtggtaca taatgcagtt
    26101 aaataaagaa gtgttaaaag gttatatcga tatattaatt gtttctatat tagaaaagaa
    26161 agattgttat ggatatgaaa ttgcaaagca agtaagagaa agaagtgagt ttgaattaaa
    26221 agaaggaaca atgtatcttg cacttaaaag aatggaatct aaaaatttaa ttaaatctta
    26281 ttatagcaat gaacaaagtt caggtggaag aagaaaatat tataatttaa ctaatgaagg
    26341 taaagatttt ttagaaataa agaaacaaga atggagattt attaaaaagg ttatgaatca
    26401 atttttggga gaggtggatt aatttatgaa actaatagat gaatatttag ataaactata
    26461 taaaaaatgt gacaataaaa gtactataga attaaaacag gaaatgagat gccatcttat
    26521 agaatctgca aatgaattta aattagaagg attagatgaa gaagaagcat gtaaaaaagc
    26581 aattgaaaga tttgatgatg gtgatgaaat gcagtatgaa ttatgtaata taataaaaga
    26641 actatcttta tcattggata ggcataaatc aattgtaatg gggtttaaaa aagtattagg
    26701 ttacataagt ataattgcat ttttaatttc tggatttatg tggtattaca ataatagttt
    26761 acaacataat atgtataatt taggaaaaga acttgatgga gaaataaagc aattggcaga
    26821 aagacatgat atgacaaaga ttggtgaata taagttagaa cttgaaaaaa tacttgataa
    26881 agataagtat agtaaagtca aagctcttag actatatgta atagatatga aagatggaaa
    26941 cactaattta agttcttctg gattgaatgc taatatggta tatgagaggg aagcagatta
    27001 taataacata agtaatttta tacagcattt aggatataat ggaaaagatt ttttagacaa
    27061 gaatgggaat atagtaaatc cagatatttt cttagaatat ttcttttatt ttgaatcgga
    27121 gatgttaatt ccagtagcat tcgtatttgg attattatgt ataatagcgt attttatatt
    27181 aagatttaaa atttctttaa taaaaaataa taattaaata attcaccaaa taattttaat
    27241 ttgtagagta aataagatgt ttcaaaataa aatttgaatc atcttatttt ttattgtttg
    27301 ttatattctt ttcttttata tttctccata gtggattgta tctatacata atcacattaa
    27361 aaacaacaca acatatgtta actggaacgc agcagaggga gccaatactg cttatacatt
    27421 ggttactaaa aaaggtgctt gttctggaat gtgtaggagc ttgaaagctt tatgtgatgc
    27481 tatgggaatt gaatgttatt atgtacatag tacaacaaat gaccatcagt ggaatcttat
    27541 tcgttttgag gatggacgtt tgtatcatgt agatattgat gccaacaaat cagcaggagc
    27601 agacatcata tacaagagtc ttactcaccc attaccattt gacaagaaag catatccagc
    27661 tgttggcgaa gtagtaaaga cacagaatcc aacgccaact gttaagaaac ctacgccagc
    27721 taagccttct gcgccagttg gacctgggaa tcacactatg atagcagatc aacaaggggc
    27781 aatacttgat gaagtttatg gaaataatct acgtccaaaa attgaaatgt atgagggagt
    27841 tccgtgtagt ttaatcaact catcaataaa tacaattaag tttacaagca aaaattcaaa
    27901 tttagaccca tcaaagcata cagttactgt aatagggttt atgaatacac catatggtat
    27961 ggttgcatta gcaccagaga gcaaacctgt tagttatcct tttgagtatg ataaggcatt
    28021 ttctttagat tttactggta agaacctttc tgcagcagaa ttatcaaaaa agtgtaaaaa
    28081 aataatataa ttattaaagt agaaattggt gaaacaacat ctaattctgg aatagggcta
    28141 gtagcatata ctagatacgt gaattaatac aagtatacta gtatacaaat agaacagact
    28201 ataaataatg caccctgtgc gaaagggtac atttattatt atattagtca ttttggaaaa
    28261 taaaaaagga gttgaactta aaattagatt agaaaaatta attttgagac aactccattt
    28321 taatttattt ttaaatataa aatcatttat tttaaataat attatgtttg attaatggct
    28381 atttaactaa ttttgcaaat tctgcaccaa atgccataca ctcttttaat gtctcttcac
    28441 tagggaagaa ttttttctta agagattcta caggcatttt gaatgacata gatttaagta
    28501 aagtctcagc catagttata ccttcaccac tccaaccaaa tgaaccaaat actcctgcta
    28561 tcttgccttg gttagccatt gggtctataa cagagaataa atcccacata ggtttaacca
    28621 tagttttgtt tatagttgga gagcctagta atattacttt tgacactact aatgtatcgt
    28681 gcatttctgt taatgtcata tcttctaaat catataattc agcctttgca ccttctttat
    28741 caagtccttc ttttatcttt ttagccatct ctaaagtatt actataagct gataagtaga
    28801 atatagaaac ttgattttga ttagttgtat taacagcttc tgtagaccac tcaacatatc
    28861 ttttaacagc agccattggg tctttagtaa gcattggacc atgagaagtc aatatagtat
    28921 caaattctat gtttagtcct accaccttat ctacagcact taaaacatgt ttagcaaatg
    28981 gcttaactat acaatcatag tagtgtttag ctgattttag ataatcttca ctattcacta
    29041 cctctgcatc aacacttgca aagtgacaac caaatgcatc acaagttaaa agagtcttgt
    29101 cctcctcaat gtaagtaaac attgtatcaa cccaatgtaa gaatggagca gttataaatc
    29161 taagatttct tttaccgata tttaaaattt caccatcttt tattacatga cagttaaaag
    29221 gtctatttat ttgaccatct aagtatagtt tagcagcttt agtacaataa acttctatat
    29281 taggatttat atctaataag tattttaaac tacctgcatg gtctggttct gtgtgatgaa
    29341 ttacaacata gtcaatttta gctatatcag taacctctga taaattactt aaaaactcat
    29401 ctttaaaatt tgcctttaca gtgtcaaata aaaccgtttt ttcgtccttg attaaataag
    29461 agttgtaagt tgtcccaaac tcagtttcca ttattatgtc gaatactttt aaaccttcat
    29521 ctactactcc agtaaagtag atatcttttt ttacttcaaa aactttactc atttttaacc
    29581 ctcctaaaat ttatcattat ataagtaaca taccccattt tgaggtgaat gaaacaaaag
    29641 atgtcatgat aagtcatgta aaacaatagt tttatgttta taatatatca tactttgtaa
    29701 aaaaattgta tttcaataaa aatttaatag aaaatattct taaatatttt tagctattct
    29761 attttgtgaa taatatgtat tttaaattca atattttagt tatatttttg atagaaaata
    29821 ttttatcgtg tataatatta cttataaata agaataaaaa taaagagctt gtttggagga
    29881 attttaataa atgaataaag tagacttgaa aaaaatctta aaaaaagtag aaaagcctgc
    29941 tagatatctt ggaaatgaaa taaattcaat tcataaagat actagtaata gtgaattgat
    30001 aagatatgca cattgctttc cagatttata tgaagttggt atgagtcact tagggagtca
    30061 tatattatat gatgtaataa ataaggatga agatgtattt tgtgagagag tgtattctcc
    30121 tgcagttgat atggaaaata taatgagaga aaaaagtata ccattatttg cattagagtc
    30181 aagagagcct ataacaaact ttgattttgt cacttttaca cttcaatatg aactttcata
    30241 cactaatatc ttaaatatat tagatttagc caatatacca atattaaaag aagaaagaac
    30301 tttggaagac ccatttataa tggttggtgg tccttgtgct tataattccg aaccattagc
    30361 agattttgta gatgtagtaa tattaggtga aggtgaagag gttaatttag aagtagtaaa
    30421 tgagtataaa gaatggaaga aaaataaaac gactagagaa gattttttat ataaaatatc
    30481 tagcatagaa ggagtataca taccaagttt ttatgatgtc aagtacaatg aagatggtac
    30541 agttcaaagt gtaactccta atagagaagg gattactaaa aatcctacta aaaggataat
    30601 aaaagatgtt gagactgtag attatccaga aaaactaata gtaccttata tagatactgt
    30661 tcatgataga atagtactag aattatttag aggatgtact agaggatgta gattctgtca
    30721 agcaggtatg atatacaggc caataagaga aaagagtgtt aaaagactta aagaaatatt
    30781 agataaatta gttaagaata ctggatatga tgaaatatca ttatcatcac ttagtacgag
    30841 tgattatagt aagttatcag agttgactga ttatttggta gatgaatatg catctaataa
    30901 tattgggatt tcgcttccat cccttagatt agataatttt tcaatggaaa ttgctgataa
    30961 aatacaacaa gtaagaaaat ctggacttac ttttgctcca gaagcaggaa ctcaaagact
    31021 tagagatgtt ataaataaag gtgttacaga agaagatttg gaaaatgcaa cagaaagagc
    31081 ttttgagatg gggtggaata gtgttaagtt gtattttatg attggtttac caactgagac
    31141 ttatgaagat ttagatggta tagctaaatt ggcttataaa gttattgata tttatagaaa
    31201 agtaaatgga ggaaagctaa agagaagttt tagtgttaca gtgagtactt ctacatttgt
    31261 accaaagcca tttacacctt tccaatggca tggtcaagat actacagaag aagttattaa
    31321 taagcagaga catttagtaa ataaattaag aaataacaat ataaaataca attatcatga
    31381 ttcaaagact agtttgatgg aagcagtagt agctagaggc gatagaagaa taggaaaagt
    31441 tatatatgat gcatttagac taggtgctaa atttgatgga tgggcagaac attttaattt
    31501 agatatatgg aaagaagcta tggaaaagaa taatctttct atagattttt atgcacatag
    31561 aaatagaaac tatgaagagg tatttccttg ggaccatata gatgtaggta ttagtaaaaa
    31621 attcttaatt agagaagacg aaaatgctaa aaaagaaaaa atcacttctg attgtagaca
    31681 taactgcaat ggttgtggaa taaatataca tgatatagga agggggcttt gctaatatga
    31741 gtaagataat aagagtaaaa tttaaaaagg aaggcgacat gatatatata tcacatcttg
    31801 accttcagag attgttacaa agagcgttta gaagagctga aataaactta tcacattctc
    31861 aaggatttaa tcctcaccct aaaatgagtt atggcaatgc tttggcttta ggggcagaaa
    31921 gtcaaggtga gtatgtagat attgaaatag aagaagatga tttatcagta gaggaatttt
    31981 taaataaagt atcaattcaa cttccagatg ggatagattt tatcaaagca aaagaaattg
    32041 atagacaaac accttctctt tcttctgtga tagactatgg agaatatttg tttaatatag
    32101 atttgaaaag acctatgacg aaagagtttg taaaaagaaa agtaattgac tttatgaata
    32161 ataaagaaat aatcataaca aaaaagaata aaaaaggtaa gatggttgaa gtagatataa
    32221 gacctatgat aagaaccttt gatgttttaa atttagaaga tgaacatatt actcttactg
    32281 ctactatagc tacaggctca aaaactaatt taaatactaa tatacttata ccaaagatat
    32341 tagagatgtt tgaacttgat attgacccat tagatgttga tatattgaga agagatttat
    32401 atgtattaga agatggggaa ttagtgactc ctatgtaagc tcgagaggtg aagttgtgaa
    32461 aaagatagtt attgagtccc taatagggtc ccagaaaaca gcagtacttg aagatgaaag
    32521 attaacagaa ctttttgttg aagacaattt aaataagaag actgtatcca atatatatcg
    32581 tggaatcgta aaaaaagtta taccaggaat agaagcttgt tttgtagata ttggatttaa
    32641 gaaacttgct tatttacaat taaaaaaggg aagtactata aaatctggtc aggatatttt
    32701 ggttcagata aataaagaag aaataggaac taaaggcgca aaattaaaca ctgagattag
    32761 tatttctgga aggtatattg tctatatacc ttccaatgat agaactacaa tatcaaataa
    32821 aattactgat gaaaaagaac gatttagatt aaaaaaaatt actaaggcag taaataagga
    32881 aaatctagga ttaataataa ggactgaggc tcaaggttgc actcatgatg aaataaagaa
    32941 agatatagaa gagttgaaat taaagtatga aaacatttta aaagaatata aacttggtat
    33001 aggtccgaaa ttattatata agtctttaga ttttgcaact aaatatgtaa aagataatgt
    33061 gaatgatgat attgaatcta taattaccaa tagttatgat aagtactctg aacttaagtc
    33121 tattttaaga ggtatagata agacttatgt tgataaactg tgtcttgagg aaaatagaga
    33181 tgtatttgat ttatacagaa tagaatctaa aatagaaaaa ttattaaata aaaaagtatg
    33241 gttaaagagt ggaggttatt taataattga aaaaacagaa gctcttacgg ttatagatgt
    33301 aaatacgggt aagtttattg gaactggtaa acttgatgaa accgtttata aaactaatct
    33361 tgaagctgca aaagaaattg taagacagct tagaattaga gacatagctg ggataataat
    33421 aatcgatttt attgatatgc ataaaaagaa gcatcaaaag gagattttac acatcctaga
    33481 agaagaattt aataaagata aaagaaaagc tgaagtatta ggtatgacaa aacttggttt
    33541 agtagaagtt gctaggcgta gagaaaaaga atccattgat aagtattatt taatgtcttg
    33601 tccatgttgt gatggtgaac aaacaataaa atctgtacat tatatactag atagtattga
    33661 aaaagaaatt atgagaataa gtgaacatac tgtttacaaa aacataatgg tagaatttaa
    33721 tgattttatt tttgaacaaa taaaagaata ttatatggat ataattgata aaattggtga
    33781 aaaatataat ataaagattt ctttaaacgc aaatagtaca ttaaagcaca ataaaaccaa
    33841 tgttattttc gataaaatag ttgacaataa aatgtaaata ttttataatt aataattgta
    33901 atgccgcaca gtaaaggttt aaatcttcaa atgaagtacc ttgtactggc gagtttgagg
    33961 ccgaggaggt gtatttaatg tacgctatag taaagactgg tggaaagcaa tataaagttt
    34021 ctgaaggtga tgtattattc gttgaaaaat tagaagctaa cgcaggtgat gttgttactt
    34081 taaacgaagt attagcttgc tctaaagatg gagaattaaa attaggttct ccagtagtag
    34141 aaggagcatc tgttcaagct aaagttgttg aacaaggtaa agctaagaaa gttatagttt
    34201 tcaagtataa agcaaaaaaa gattacagaa gaaaacaagg acatcgtcaa tcatacacta
    34261 agatagttgt agaaaaaata aacgcttagt tattggttaa gttatgataa aagttaaata
    34321 ttattataat gatgatttct tgttgcaagg tttttgttta aagggacatg ctgattttgc
    34381 tgaaataggc tatgatatag tatgtgctgc agttacatct aatgctattg cagttataaa
    34441 ttctttagac aaattacaga agattgaatt tgaaaaagta gtaggagaag aaggtcatat
    34501 agagtgtata gtcaaagata cacatgtaaa agacgcacaa ttattactaa atcactttca
    34561 attagcggtt aaagaaatta aacgggaata cccaaaaaat ataaaaattt taaaaaagta
    34621 gggggtgact ccatatgtta aacatgaatt tacaattatt agcatctaaa aaaggggtag
    34681 gttcttctaa aaatggtaga gattctatat ctaaaagatt aggtgttaag agattcgatg
    34741 gtcaattagt aactgctgga agtataatag ttagacaaag aggaactaaa atacatccag
    34801 gaactaatgt tggaaaaggt agtgatgata ctttatttgc attagttgat ggtactgtta
    34861 aattcgaaag aaaagacaag aagagaaaaa aagttagtat atatccagta gcaatagctg
    34921 agtaatatac ataaaaggag tgttgaggca ctcctttttc ttataatact gatgaattta
    34981 taatggtatt tttggtaatg ctaattaaaa gtttttaatt aagtaggtga agaatttgtt
    35041 tatagataag gcaagaatat ttgttaaggc tggaaatggt ggaaatggtt cggtagcatt
    35101 tagaagagaa aaatacgttc cagctggtgg tccagacggt ggagatggtg gtcgtggagc
    35161 aagcatcata tttgaagttg atttggggct tagaactcta atggacttta agtaccagaa
    35221 aaaatatcaa gctcaaaatg gtggcgacgg aagcaaagga aaaagagctg gtaaaaatgg
    35281 agaaaattta gtattaaaag taccagcagg tacagtaata agagatgagg ctacaggtct
    35341 tgtattagca gatttaaaaa aagaaggaga tacagctata gttgcaaaag gtggtatagg
    35401 tggtaaaggt aaccaacact ttgcaaatgc agtaagacaa gctccagctt ttgcaaaatc
    35461 tggtacagat ggagaagaaa gatggataac tcttgaactt aaaatgatag cagatgttgg
    35521 tcttttagga tttccaaatg taggtaaatc aacatttcta tcagttgtaa ctaaagctaa
    35581 acccaaaatt gcaaactatc attttacaac acttacacca aatctaggtg ttgttcaaac
    35641 taagtttgga gatagttttg tacttgcaga tatacctgga attatagagg gtgcttctga
    35701 aggcatagga ttaggtcatg agttcttaag acatgttgag agaacaaagg tattgataca
    35761 tattgtagat atatctggat tagagggaag agaccctatt gaagattttg acaagataaa
    35821 tgatgagctt aaattgtaca atgaaaagct ttcaaagaga ccacaagttg tagttgcaaa
    35881 taaatttgat atattagaag atgagagtaa gtttgagaag tttaagagtg aattagaagg
    35941 cagaggatat acagtattta aaatgtcagc agcaactcgt caaggcatag atgaagtaat
    36001 agcctatgtt tctaaaatgc taaaagaagt agaagatgtg gagctagttt cagaagaaga
    36061 aatgtataga ccagaacttg atattggtac tgaagaagag ttaagtatag atattgaaga
    36121 tggtgtgtat gtagttacag gtaaagcact tagaagaata atgtattctg taaattttga
    36181 tgatatggaa tcccttcaat atttccaaaa agctatggag agtcaaggtg tttttgatag
    36241 gcttagagaa atgggaatag aagatggaga tgtggttaaa atttatgaat tagaatttga
    36301 gttttacaat taatttaaat atagattata gataatttaa gtatagaatt atagaggtga
    36361 gaatatgtta aaaggaaaac aaagggcgta tttaagatct ttggcaaata ctttaaagcc
    36421 tactacacaa attggtaaag aaggtgttac agagagtttc ttagagcaat tagatggtat
    36481 gttaagaact agagaattag taaaagttac tatattagaa aatgcaggtc ttgatactaa
    36541 agaaactgct aatgcagtat gtgaagcttt aagagctgaa tttgtgcagg ctattggttt
    36601 taaatttact atttacaaga ggaatattga agagcctaaa atactttttc caggacatga
    36661 acaggcgaaa gcaaagacta aaaataataa tgttacaaaa aagggtaaac caactaaaag
    36721 agctgttaga taatagttta tatcctttta gggaaataag ttaaaaaaat agtgacaatt
    36781 ggaagaaatt gtattatact attaaacata aatttaaatt agcaatctta tcaagagtgg
    36841 cagagggact ggccctgtga agcccggcaa ccagtatatt taattataca ttggtgctaa
    36901 atcctgcagc atttatgctg aaagatgagt ataaacccgt catatggctt gagtttatac
    36961 tcaagctttt tttattggta tatttatata tataaataat ttaatataat caaaatgatg
    37021 ggggtatgtt acagtgaaga tagcaatatt agggtatgga acagtaggaa gtggattaat
    37081 tgatttaata gaaaataata cttcaaaaag agatatagaa gttgttggaa ttttagttaa
    37141 aaataaagaa aaacacaagt ataagaagta ttttgataag ataactactg atatagaaga
    37201 tatatttgac aaggatattg atatactagt tgaagtaatt ggaggattaa atccagcttt
    37261 tgattatgta acaagagcgt taaataaaaa aatacatgta gtgacagcta ataaagattt
    37321 actggctgaa aaaggtagtg accttataga attagctaac ttaaataatg ttagcatcaa
    37381 gtttgaagca tctgtggcag gtggtattcc agttttaaaa ccattgatag aatcactgga
    37441 aggcaacaat ataaagagca taaacgctat tttaaatggg acttgtaatt ttatattatc
    37501 aaagatgtat gatgaaaatt taccgtatga tgtcgcttta aaacaggcac aagagttggg
    37561 atttgcagaa gttaatccag atgcagatgt tttaggatat gatagtgcta gaaagttatc
    37621 tatattgtct actttatctt atggaaaaat agtatattgg aaagatttat tattagaagg
    37681 aataacttca atagatgaaa aagatataga atatgcaaaa aaattaaatt gtaagataaa
    37741 attggttgct agaagtaagt atgaatcagg agaagtaagt ggatttgtta gaccagcatt
    37801 agtggacaat aataatatgc tttcaaagat agataatgag ttcaacgtag ttattttagt
    37861 aggtgattca gtaggtgaac tttcttttgt aggtaaggga gctggaagag gtgcaacagg
    37921 aagtgctgtt tatgcagata ttatagatat aatagataat agaagttcaa atataaagtc
    37981 tttttcaaaa gggaaattgg atttaagtgg acttatagaa gatgagtgta gtgcagtagt
    38041 tagatttggt gggtataata ataaagatgg aattttagaa tgtttagata aatatgtaga
    38101 tagttatgat attatagatg atgaagaatt ggcgattttt gtaagtgcaa agtcagaaca
    38161 tgagattgat agagttttgg aaattgtaaa aagaaataat tacagtgaat cagctaaaaa
    38221 attattgaag atagattaat atgtttatgc aagaattatg aaaatttaat agggtgtctc
    38281 aaaatagaga gataagcctt ttttccagtg ttgaaataat aaaaatatat ttgattttat
    38341 aaaaaaagag tgtctcatga actaaaaagt tcattttgag acactctttt ttaatatctg
    38401 gtgaattaat aagataatag aaaaatgtat acgttataat taaccaataa agaaattttc
    38461 actagcttca ttaatagctt gctgagttac ccttatatat cgctgtgtag tggctacaga
    38521 gctgtgattt aaaagttctt taaccaactc taaattattg ttactctttt caaattgagt
    38581 agtagcatag agctttctaa agctatgtgt acttatatta tacagattta agtatttaca
    38641 aattatcctc aactgttttt gaatagcctt tgtctttata tttactaagt agtcatttga
    38701 tttaagatta tactttcttg catgttcatt tatggactca attatatttt tattaactgg
    38761 tctatactgt aatttgcccg tctttttttc atttatttcc aacatattac ctttaaaatt
    38821 acctatcttt aatcttaaaa tatctgatat tcttagacct aaattagctt ctaacattag
    38881 tgccaatgca actttaggat tttttctaaa tcttttttca acgccatttt cactataagt
    38941 aaagccagta tgtaatagtt ccattatctt tttatattcg tctatttcta taggtcttgt
    39001 tgccattttc aaccactcct cttagtttga tttggtaagt ttaggtcggt atttattata
    39061 taatataaat gaaatattat ataatggaaa aatttcccag taaacttata caagtgtgat
    39121 atatgtgatt aaggagaatt ttttgaaaaa tatttctaat aaaattatta aattgtgatg
    39181 aaataactat gctgttaaac taattaaaaa ggttattaaa attatgacaa atataataaa
    39241 aaaatggaag tatttagtta agttttatta gttttatttt tagttttatt tttttcgtga
    39301 aaatttctta gtgaaaaaag tatttttata tcgcattagt ttcccatttg tagataaagt
    39361 tttcatatgt ttttttgcaa taaatacggc tgagattaga taaattatta ctacaatttg
    39421 aagaattgta tcataatagc taagataata aaaccagatt aaattattat atataacgtt
    39481 gtatgtatca gcaaagctag aaaccaacac agcagagttt gcaattggat aaagtataaa
    39541 tataaagata gcagctaaaa ctccatgcgc aaatttactt agtatgtata attttaaatc
    39601 tatgtccgtt tttgatagaa atgtacaaca ttgagctagg atggataatc cactaaatcc
    39661 tatcaaaaaa ctagctaaag atgctcttaa aatttctgga gaacttgtta tactagatac
    39721 tttattgcat cctatagtaa tttcaaatag accacttaca aatgcttgac agagttcttt
    39781 actaacacct aaaaatgaaa gaggcaaata tataactgat gctattaatg agattacatt
    39841 aaaaagagat agtattttaa atactactga aaaaacaatt acaaatccac caactgtaag
    39901 taatgtattt actccattaa atacagcatt tccaaataaa acaaaaaaac cattcccact
    39961 tgaatttcta atgttgatta cattttttat atttgttttt aaatccaatt ttgtctttgg
    40021 aagattttca ttaccataat ttctaaagaa aagaccaacc aatatagttc ctagataatg
    40081 gcagagaatc attaaataac ctaagtttga attttggaac atacctgtac caaccgaacc
    40141 tattatgaat aaaggtcctg atgtagaaca aaaagacaca agtctttgtg cttcatactt
    40201 agaaatttgt ccattactcc taaattcaga agctaaactt actcctactg gataaccaga
    40261 tactgtagat atagcaaaag ctaaagcgct ctttcctgaa acattaaata caaatctagt
    40321 tatgggattg atgataaacc ctataatatc cactattttt aaatcaacaa ttaagtttgc
    40381 tccaattata aatggtagta gtgatggaac taaaacatta gaccatatag aaaagccttc
    40441 tcttgcagat tttatagctt catttgggta taggataatc cctaaaacta gaactccaac
    40501 tatgatagat ggagcaaaaa aagcaataat tttatttttt ttcataatat cacctgtaaa
    40561 tttgagttat aagatattat attaaaagta ataaaaatat atgaatgtaa tggcttaaga
    40621 tatataatag atatatatta caacaacgat tatcatattg gaggaaaaaa tgtttaatag
    40681 tatagctagt aaaaaagttt atgaacaagt aattgagcaa atacaatata aaatactaaa
    40741 tggagagtta aaaaaggggg ataaattact atcagaaaga gaactgtctg aacaaatgaa
    40801 tgtgagtaga acttccataa gggaagctat tagggtatta gagactatgg gagtgataga
    40861 aagcagacag ggtgaaggaa attttatatg tacaaatata gaaaaaactt taatagaacc
    40921 tttatctatg atttttaaac ttaataatgg tacacttgaa gatatattag agcttagaat
    40981 aatcctagaa attgagattg caaaacttgc ttcaaagaga ataacttctt ctgaagttat
    41041 agaattgaaa catataatag atgaaatgag ggtagaaact aataaaaaag ataataatcg
    41101 agtactggtg ttattagacc aaaaatttca cagtaaacta gcaacattaa gtaaaaatta
    41161 tttaattcaa agtcttttta tgacagcatc aaaattattt gatggattta tagaagatgc
    41221 aagagaaaaa ataatcgcag aaccttttaa tgaaaatata ttgcttaaac agcatgaagc
    41281 tatatacaat gcagttgtag aaaatgatgt agaacttgca tgtgagaaag caaaggaaca
    41341 tatggatttt ataagtaaaa attatagaaa aaatgaaaat taaaagtttg ttataaaata
    41401 aattattctt gcaaatggta gataatggta ctatcatata aatgtaaaac tggtatgacc
    41461 acataccaat tttatagttt attataatgg gagattaaaa atggtaaagt taaaagtacc
    41521 gtattcaaaa caaggaatga ttttagaaat acctgatgaa cgttttctcg gtgttttgga
    41581 gtcaaaatcg gataattata tagaacataa gactcaagaa gagatagttg aagaatctat
    41641 gaataatcct ataggctcta attctctgga agaactggtt aaggggaaaa agaatgtagt
    41701 cataattaca agtgaccata caagacctgt accaagtaga ataactatgc caattatatt
    41761 aagaaggatt aggaatgtta atccagaaat tgatgtgaag attatagtgg caactggatt
    41821 tcataggcca agtacaaggg aagagctaat ttataaaatg ggagaagaaa tagtagataa
    41881 tgaagatata gttatgcata tatctactga tgataaatct atgtgtaaag tagggatatt
    41941 gccttcaggt ggagatttat atataaacaa actagcatat gaggcagaac ttttgatagc
    42001 agaaggtttt atagaacctc atttctttgc aggtttttca ggtggcagaa aaagtgtact
    42061 tccaggaatc gcatctgcaa agactataat gtacaatcat tgtagtgact ttatagatag
    42121 tgataattca agaactggaa aattgaataa taatcctatt catgaggata tggtatatgc
    42181 agctaaagtt gcaaaattag cattcatatt gaatgttgta atagataaag ataaaaaaat
    42241 aattgcttcc tttgctggtg atgtcgaaaa agcacatact aaaggatgta gatttgtaac
    42301 agagttatca aaagtaaatt ctataaaatc tgatattgtt gtaactacaa atggtggata
    42361 tccactagac caaaatattt atcaatctgt taaaggaatg acagcagctg aagctacttg
    42421 taaagacggt ggagtaataa taatgatttc agcatgtaat gatggtcatg gaggtcaatc
    42481 tttttatgaa aatgtagcaa atgcagattc tccaaaacaa cttttagata aaattagaag
    42541 tgtcgataga gtagatacga ttccagacca atgggaattt cagatactag cgagaatatt
    42601 atctaaatat actgtgataa tggtaacaga taagtgtaat cctgaaatga taaaaaaaat
    42661 gcatatgaaa catgcattta actttaaaga agctttagaa atggcgaata gtatagttgg
    42721 taaggagagc aaagtagtag ttatacctga tggagtatca gttatagtta aataaaaaag
    42781 gagtgtactt tatggaaatt ttgacatgta ttaaacaagt tcctggaacg acttcagtag
    42841 aagttgatga aactacagga gtactaaaaa gagatggagt agattcaaaa atgaatccct
    42901 atgacctata tgctttagaa acagctctta gaataaagga agataagaaa gcgaatctaa
    42961 aagtacttag catgggtcct cctcaggcaa agaaggtaat tgaagaaagt tttatgatgg
    43021 gagctgatga aggagcatta ataagtgata ggagatttgg aggagcagat gtattggcta
    43081 cttcctatac catatcacaa ggtattaaga agatgggaaa ggtagaccta ataatttgtg
    43141 gaaaacaaac tactgatggt gatacagcac aggtgggacc tgaagtagct gaatttttag
    43201 atatacctca tgttactaac gttacaaaat taattgaagt taaagatgaa agtatagttg
    43261 ttgaaataga tatgccaaat gatttacaag tatgtgagat tgaatatcca tgtttaataa
    43321 ctgtagaaaa agacattttt caaccaagac taccttcatt taaattaaaa cttaatacta
    43381 aagatagaga aatacctgta tatagcttag atgactttga agataaagat gaaaataact
    43441 atggacttaa tggttctcct actcaagttg taagaatatt tcctccaaag ccaaatacag
    43501 ataaaaatat tgtaagagga aatgcagatg aactaagttt tgcacttgta aataaattag
    43561 aggaattaaa gttggtgtaa gggggagtaa taaatgtcaa agatagttgt aaatcaagat
    43621 aaaataactg acttaaagag aattttagaa atatgtcctt ttggggctat agaagaaaag
    43681 tcaggtatag tagaaataag tgctggctgt aagatgtgca agttgtgtgt caaaagtgga
    43741 ccaaaaggcg catttgaatt tatagagagt agtaaagtac aaattaataa agatgaatgg
    43801 aggggaattg cagtatatgt tgaacatcat aatggaaata tacatcctgt aacgtacgag
    43861 cttataggta aagctcgtga gatggcatca aaaataaaac aacctgtgta ttgtgtattt
    43921 gtaggaaaag acataaaaga taagtgcagt aatttgattt cttatggggt ggatgaagta
    43981 tttgtatatg atgaagatga atttaaagat tttaggatag aaccatattc aaaagcaata
    44041 gaaaacttta tagataaaat aaaaccaacg atagtattag ttggtggaac tacattaggt
    44101 aggtcattag ctccaagact tgccgctaga tttagaacag gtcttacagc agattgtaca
    44161 atacttgata ttcaatcaaa tactgattta gaccaaataa gaccagcatt tggtggaaat
    44221 ataatggctc atataaacac acctaataat agaccacaat ttgccactgt aagatataaa
    44281 atattttcag caccagaaaa aatagagaat actacaggaa agataacttt atgtaaagta
    44341 gaaaaaaaag aactaaaatc taaaattaag gtactgagtg taaaagaaaa aaataaagaa
    44401 gttggtctgg aagaagctga agtgatagtt gtggcaagta gagcaataaa aaaacaagaa
    44461 gatatggaga tgatatataa attagctgat aagttaaacg ctcaagtggc aggaacaaga
    44521 cctgtaatag aagcaggttg gatagatgct aaaaaacaaa taggtcttag tggtagaact
    44581 gtaaaaccta aattgataat aacttgtggt gtatctggag cagttcaatt tgttgcaggg
    44641 atgcagggtg ctgactatat agtagcaata aataaagatg ataaagcgcc tatacttgat
    44701 gtagcacatt tagctttaat aggtgatatt tatgatatta ttcctaaatt gattgaaaaa
    44761 atagaaaata acaaagataa taatcaaaaa tatatggcat cagcagctaa atagggggat
    44821 agaaatgtat aaattaatcg ataaaaaaga tatagatttt ttaatagata cttgtggaga
    44881 agaaaatgta ttagttggaa gtgatataaa tgaagatttt tcacatgatg aacttggagg
    44941 gattgagaag taccctgaag ttttagtaaa tgtacttgaa actgaacaag tatctaaaat
    45001 aatgaaatac gcatataaaa ataatatacc agtcacacca agaggtcaag gtactggtct
    45061 tgtaggtgct gctgttgcca taaatggtgg aataatgatt aatctatgta aaatgaataa
    45121 aatacttgaa gttgattatg aaaatttaac attgacagta gaaccaggtg ttttattgat
    45181 gacaataggt caatatgttc aagatagaga tttattttac ccacctgacc caggtgaaaa
    45241 aagtgctaca attgcaggta atataaacac taatgcaggt ggtatgagag cagtaaaata
    45301 tggcgttaca agggattatg taagaggact tgaagttgta ctaccaaatg gagaaattat
    45361 aaatgtaggt ggaaaggtgg ttaaaaatag ttctggatat agtataaagg atttattagt
    45421 gggctcagaa ggtactttag gaatagttac taaagctata ttaaaattgc ttcctttacc
    45481 aaagaaaagt ataagtttgc ttataccatt tccagattta tcaatggcaa tagaaactgt
    45541 tcctaaaata ataaaatcaa aatcaatacc aacggctata gaatttatgg aaagagatgt
    45601 gatattggca gcagaagaat ttttaggtaa aaagtttcca gataatacat cagatgctta
    45661 tcttttgctt acatttgatg gaaatagcac tgaagatata gaaaaagaat atgaaaaagt
    45721 agctaattta tgtttagaaa atggtgcatt agatgtattt atatcagata cacaagaaag
    45781 aaacgattct atatggtcag ccagaggtgc atttttagaa gctataaagg catctacgac
    45841 acaaatggat gaatgtgatg tcgttgttcc aagagataag atagcagagt ttataagata
    45901 cactcacgag cttcaagaca aattaaaaat aagaataaaa agttttggtc atgcaggaga
    45961 tggaaatcta cacatatata ttttaaaaga tggtatggat gataatacat ggaaaataag
    46021 attaaaagaa acttttgact atatgtataa aaaatctaga gaacttagtg gtcaagtttc
    46081 tggtgaacat ggcataggat atgctaaaaa agagtactta catgaatcaa attcagatgc
    46141 ttatatgatg ttaattaaaa atataaagtt agcttttgac cctaaaaata ttttaaatcc
    46201 agggaaaata tattagaaat att
//
LOCUS       DAYAOX010000004        46192 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.004, whole genome shotgun sequence.
ACCESSION   DAYAOX010000004 DAYAOX010000000
VERSION     DAYAOX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.004"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            342..464
                     /locus_tag="ACGI57_000160"
     CDS             342..464
                     /locus_tag="ACGI57_000160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715251.1"
                     /translation="MMDLEKEKHEIIEIIKKLNIDKDKRNLLMIKNYILGLLKK"
     gene            complement(479..865)
                     /locus_tag="ACGI57_000161"
     CDS             complement(479..865)
                     /locus_tag="ACGI57_000161"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9715252.1"
                     /translation="MIDEMKPENKRIRELRLNLNLSQEEFANKLNLKRNTISLIENGK
                     RNASERTLNDICKTWSISEEWLKHGVGEMFFIKELDEQFAHLVGELSKMENEVEREII
                     TKMFKLNKKYLTVFDKMIDSLLEIEE"
     gene            1013..1156
                     /locus_tag="ACGI57_000162"
     CDS             1013..1156
                     /locus_tag="ACGI57_000162"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715253.1"
                     /translation="MTDINKIREEIYRKLELLGPAKLKLALTKVDILKEIQDIEDENN
                     IQN"
     gene            1161..1385
                     /locus_tag="ACGI57_000163"
     CDS             1161..1385
                     /locus_tag="ACGI57_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715254.1"
                     /translation="MKRLKISDFNKNQIGSAVQLQDIRFDTKADCIITKVEKNEILVM
                     YYEKETEEIAYKTLTKEDLILDDYKLKLLS"
     gene            1404..2144
                     /locus_tag="ACGI57_000164"
     CDS             1404..2144
                     /locus_tag="ACGI57_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9715255.1"
                     /translation="MITLDLLKDARKKEWFWLENDLIDREDLSIYEKMIYIVLARYSN
                     EESSCFPSYKTISEKAGCSIRQVSNILNELENKELIIRENRNHEGKKEKNSNLYFLIS
                     SKAKVQNEVPNVRHEVPNPMAPGALHVRHEVPSKKTYNKKTYIKSNTTTQKESKTDYL
                     DLSFLDLDIEKVKLTKDEYDKLISKFGKKYIHDKIVSLENYIINGKGSRYKSHYRALL
                     TWGNGDASKGIVPEVTKTKNPLSGFKEL"
     gene            2172..2357
                     /locus_tag="ACGI57_000165"
     CDS             2172..2357
                     /locus_tag="ACGI57_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715256.1"
                     /translation="MNEKEETVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFDIKSDI"
     gene            2383..2601
                     /locus_tag="ACGI57_000166"
     CDS             2383..2601
                     /locus_tag="ACGI57_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715257.1"
                     /translation="MKDEKLELTEKDLHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECKDYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(2620..2826)
                     /locus_tag="ACGI57_000167"
     CDS             complement(2620..2826)
                     /locus_tag="ACGI57_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715258.1"
                     /translation="MCKIKTLREKCSLIDDKVEFSGEYKTFPGFTGSKLLECECNKNY
                     ICKIQIMTANSNCPLFKKARQLQY"
     gene            2995..3255
                     /locus_tag="ACGI57_000168"
     CDS             2995..3255
                     /locus_tag="ACGI57_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715259.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            complement(3248..3436)
                     /locus_tag="ACGI57_000169"
     CDS             complement(3248..3436)
                     /locus_tag="ACGI57_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715260.1"
                     /translation="MSQYRVISKECPVINETISLTCEYSAPPGTFGSKLMSTRCDGSH
                     ICDSSSNEECPILKKLLR"
     gene            3574..3891
                     /locus_tag="ACGI57_000170"
     CDS             3574..3891
                     /locus_tag="ACGI57_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715261.1"
                     /translation="MERKKHEFTDQDIQRLIEGLRTDPKIREMIFKSKGCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            3913..5244
                     /locus_tag="ACGI57_000171"
     CDS             3913..5244
                     /locus_tag="ACGI57_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFG9715262.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKKIIDSNKPLDLLLLKNELEKIDRLVDIGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIIDINDLKALVTTSSSVNDNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAQAITNGEKAFLYSG
                     ELPSFMSMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYAVELISDWIEDKFFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRFFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVVDEEEETDDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQVEIPEDVF"
     gene            5619..6005
                     /locus_tag="ACGI57_000172"
     CDS             5619..6005
                     /locus_tag="ACGI57_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715263.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKATIVEAKATTTKVLIGKDLETGRVEASEFKNQVAGQLVMD
                     IPDSEETNETEENSAGVIDFRNASSK"
     gene            6058..6762
                     /locus_tag="ACGI57_000173"
     CDS             6058..6762
                     /locus_tag="ACGI57_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715264.1"
                     /translation="MIRNAMEYLVKLGKRETVCVDGFKYTTDALEIIKEPKANELEIT
                     TLSGLVDYIKSGIDHKADELLLIQVLTPRKVLLKSPLRKNRDRETYIECVALLPEKRF
                     DRPLDLDSFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEIEQPESEFIFRMSSGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            6819..7001
                     /locus_tag="ACGI57_000174"
     CDS             6819..7001
                     /locus_tag="ACGI57_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715265.1"
                     /translation="MKMISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            6998..7375
                     /locus_tag="ACGI57_000175"
     CDS             6998..7375
                     /locus_tag="ACGI57_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9715266.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYTKKDGTR
                     EVDFIPIEVMGGSAEFCANYLAKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DRKPKDEEKEISFQVLDDEDIPF"
     gene            7392..7940
                     /locus_tag="ACGI57_000176"
     CDS             7392..7940
                     /locus_tag="ACGI57_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9715267.1"
                     /translation="MRSLEERNKLVEENINFVWFIIRRYFKVFISKYPYLQDDTFSEG
                     CIGLCKAADYFDSKKGIEFTTFAAVCIKGQIFKFISRYVKKHYRADIDSFEKCIYKNT
                     HGEEIRIEDRLAGNENVDIENVHVIRSCIKRSKIEDIQKITYLREKGYTQKEIGKILR
                     TNQMTICRKLGKLKSEINILGK"
     gene            7970..8128
                     /locus_tag="ACGI57_000177"
     CDS             7970..8128
                     /locus_tag="ACGI57_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715268.1"
                     /translation="MKTKIIGGRENQCPLCNGSVFKIETSIGIVWQCKDCGCMYQDMN
                     CKENKRRH"
     gene            8131..8457
                     /locus_tag="ACGI57_000178"
     CDS             8131..8457
                     /locus_tag="ACGI57_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715269.1"
                     /translation="MACVIKCDFCGETIAEILNIKVFRIDDEGNIMSIGKDVCDKCYD
                     RLFRSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQECLGGCLEGRKKWL
                     ESEAMK"
     gene            8457..8993
                     /locus_tag="ACGI57_000179"
     CDS             8457..8993
                     /locus_tag="ACGI57_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFG9715270.1"
                     /translation="MKKTLIIVGVVISILIMFACLFLVTKNKAIDLEEQVQSATASVE
                     VQEKRRIDLIKNIVDCVKDYAKYENRTLKEVTDMRVSANDESIKEAKLNIQAVAEKYP
                     DLKANENYKQLMSELSITENMIAQHRENYNMQVRNYNKYIRKFPANLILNSMGYEKIE
                     SKYTEYKAKEDAPQNLFD"
     gene            9005..9811
                     /locus_tag="ACGI57_000180"
     CDS             9005..9811
                     /locus_tag="ACGI57_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715271.1"
                     /translation="MKFKTFEITKREFLVSIIIACMMIVLGFFIDDKVQSKFLEDNER
                     YYKATKINNDKDLFDYAMETSAGDSLISGRFKSVDSVSIEELKGDYWFIERTEEKYTM
                     HTRVVTTKDSKGHSHKYTETYWTWDYHDSIEKKCNKFNFMGKEFDAKVITNLSKERLA
                     LKKDIFKNNNLKIKGNYAYINNKRRYYYEVIPKEFNGTMYANLKNNTIANPADKNIKV
                     FKGESIQNVLENNNFNTGFFKVVFWIIWILITTIIIYVFYYLKNDWLEDK"
     gene            9836..10096
                     /locus_tag="ACGI57_000181"
     CDS             9836..10096
                     /locus_tag="ACGI57_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715272.1"
                     /translation="MKCKVYHAIKNIKDANELIDRFETENIFFELNDIDNENVLNGEM
                     NLTDIEDIASLQFILDGDLFIFSSCDEMIFMNKYTQKELGLQ"
     gene            10120..10605
                     /locus_tag="ACGI57_000182"
     CDS             10120..10605
                     /locus_tag="ACGI57_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715273.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKNAIDTGITKDIIAEG
                     LDVCQTILTMFQVLEIENYITEGVETHNKKLNGRGWDLKEIGDDILNLIKNSMINELI
                     NMNKKLKCLDEKEFNAKIQFLDVDKLWNLCMPLVNLSTIKGVNESIDEMKMGEFLKNI
                     N"
     gene            10625..10882
                     /locus_tag="ACGI57_000183"
     CDS             10625..10882
                     /locus_tag="ACGI57_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715274.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPRE"
     gene            10991..11434
                     /locus_tag="ACGI57_000184"
     CDS             10991..11434
                     /locus_tag="ACGI57_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFG9715275.1"
                     /translation="MQSSKKDNLFSSAEGKLYDYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTGKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            11577..12122
                     /locus_tag="ACGI57_000185"
     CDS             11577..12122
                     /locus_tag="ACGI57_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715276.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDITEEISSAAYVPDNLKYYDNVM
                     KETPGMYGRECSLDFIKKKQEKLLKLKKKVKKNYDSNINKIDSYLKILEKSIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFTDVVE
                     DLKILVQERQRKNYYGNKECF"
     gene            12126..12770
                     /locus_tag="ACGI57_000186"
     CDS             12126..12770
                     /locus_tag="ACGI57_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9715277.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RVGDILALKVADVTDLKLDKKGNKIRVSKDWIRVIEEKRDYNREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAKKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            12991..13443
                     /locus_tag="ACGI57_000187"
     CDS             12991..13443
                     /locus_tag="ACGI57_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9715278.1"
                     /translation="MNEVEIIDSSYEKGKELTEDQCNMVTLLITGATVTETARIIGVN
                     RKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVMLA
                     ANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            13998..15737
                     /locus_tag="ACGI57_000188"
     CDS             13998..15737
                     /locus_tag="ACGI57_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715279.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFPILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLSKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
     gene            15753..15941
                     /locus_tag="ACGI57_000189"
     CDS             15753..15941
                     /locus_tag="ACGI57_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715280.1"
                     /translation="MANRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRARSKSY
                     ISKQDIPIKVLKFIESTG"
     gene            15954..17231
                     /locus_tag="ACGI57_000190"
     CDS             15954..17231
                     /locus_tag="ACGI57_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_process: GO:0099001 - symbiont genome ejection through
                     host cell envelope, long flexible tail mechanism [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9715281.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNILSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            17209..17970
                     /locus_tag="ACGI57_000191"
     CDS             17209..17970
                     /locus_tag="ACGI57_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFG9715282.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            18029..18355
                     /locus_tag="ACGI57_000192"
     CDS             18029..18355
                     /locus_tag="ACGI57_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715283.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            18381..18983
                     /locus_tag="ACGI57_000193"
     CDS             18381..18983
                     /locus_tag="ACGI57_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFG9715284.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            19012..19935
                     /locus_tag="ACGI57_000194"
     CDS             19012..19935
                     /locus_tag="ACGI57_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715285.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            19949..20221
                     /locus_tag="ACGI57_000195"
     CDS             19949..20221
                     /locus_tag="ACGI57_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFG9715286.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            20205..20585
                     /locus_tag="ACGI57_000196"
     CDS             20205..20585
                     /locus_tag="ACGI57_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFG9715287.1"
                     /translation="MSTMLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELS
                     TALEGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENS
                     ISSSSFLTDTDKKFLSPFCIVSMY"
     gene            20597..20944
                     /locus_tag="ACGI57_000197"
     CDS             20597..20944
                     /locus_tag="ACGI57_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715288.1"
                     /translation="MTEADILEMTYLDKMTIIRKLKGWNEETCSNDFSNVVIAENIPC
                     AISRKEEAIVNGDIGSIVVNRKLFCRPEVDIKIGDTINTTYNHGEEAVFKVSKANIYP
                     SHMEIPITELERI"
     gene            20944..21300
                     /locus_tag="ACGI57_000198"
     CDS             20944..21300
                     /locus_tag="ACGI57_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFG9715289.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTKETGQELAENIRD
                     ETPVKTEKLKKGWEEKQPEYDTYIVKNEVDYATLVNNGHSTKNGGIVPGSFMVEKGIE
                     RTKENMTSAKANLFDW"
     gene            21309..21758
                     /locus_tag="ACGI57_000199"
     CDS             21309..21758
                     /locus_tag="ACGI57_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFG9715290.1"
                     /translation="MLTNRDIIIAVNKRIENEFRYIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKVLDTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLIIE
                     NISPSIAKDPVGYKLTFLMTVSYFEEVYFENETFEIMESVDLEIGSD"
     gene            21759..22814
                     /locus_tag="ACGI57_000200"
     CDS             21759..22814
                     /locus_tag="ACGI57_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFG9715291.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEIE
                     DIPENYSAANLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMPD
                     ETEQDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDEGSTVEINLDAQRAWLKKQNIDISNMEEQQIKEYNTDTLVFLKA
                     RIKLIDAMEDVYLDISM"
     gene            22830..23270
                     /locus_tag="ACGI57_000201"
     CDS             22830..23270
                     /locus_tag="ACGI57_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFG9715292.1"
                     /translation="MKAKIDAKRIISGTFGECWLDNKQLTSTKGMEAKVDIDKEEIKL
                     CGQIMTTYKMIGATGKGSITLYKISTLFGEYVEKMLNEGKEFKVTIISKLQDPDSYGS
                     ERIALYGCMFDDLTLADWEAAKVGEIEVPFTFEGFEYLDKIKEK"
     gene            23285..23701
                     /locus_tag="ACGI57_000202"
     CDS             23285..23701
                     /locus_tag="ACGI57_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9715293.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSAFELYCKKLDDTLEIECKAIDPER
                     FDEIRMNSMDVNSGDVENINIYELKVNTILESCSIFSNMELVKKFSAITPKELIKKLL
                     LSGEIDKLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            23728..23877
                     /locus_tag="ACGI57_000203"
     CDS             23728..23877
                     /locus_tag="ACGI57_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715294.1"
                     /translation="MYLLFKYKGIMPSVSYHILRGEKEIVSVFMDQELDERKEEIKSG
                     VGRLF"
     gene            23881..27270
                     /locus_tag="ACGI57_000204"
     CDS             23881..27270
                     /locus_tag="ACGI57_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFG9715295.1"
                     /translation="MSSNTKVLQAIIKMRDEASSTLKRVAANTRALHDSSSQASRALN
                     NVQSNLKSVGTSALKAGGLITAGVGGAFAAATKVGTEFESAMSQVKATFGNSLKPGDF
                     EALSKAARDAGKATTKTAADSANALQYMGLAGWDVQKSMHALMPVLNLSEAASMDLGL
                     ASDLVTDSMSSMCLEVDKVSKSGKVLAGAGLSEYLDKVANASTKSNTSIQQLMEAFNV
                     AGGTFSNLKVPLSEATAIMGILANRGVKGTDAGHALNSIMVNLTTGAGQAGKAMKKLG
                     VSAFDNQGKFKGMSNVLKEVANKTKGMTDEQKNYYLSAIGGKTQLKTLQKLLDGVGNE
                     YDSLKKKIDNSKGALKTMSDVMKDNTKGGLATLKSALEETGITIYYQLKPAIASVVDN
                     LTKMTVWFNNLSEPIKRVITNVMLSVGAFGVLLLTIGAVSLIVSKAIGIFGFFVPIIL
                     RAKVAFRAFVIGMKPLSAVFLAVMGPVGWTITAVIALSVAFTIAYAKSEVFRNKVNSL
                     VKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIGV
                     IGFHIAKLVLILSTLATAFKAVVDTITNVLNGLASILNGVFDLIIGIVTGDSKKMMDG
                     VKQIFDGGTKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWRDLRTFLEN
                     PIEATAKISTKTNVNIPGSKAADAAGKSTPNTGGLKQTVANLKGSFSDFKTFIKPMTD
                     SISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELKN
                     ILAGNIKTNIDNLVKAFEPLKPHLDNLKDCFDKVKKAITDFFTPAKQVGDTVNKVSQP
                     MDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGGI
                     ILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDSG
                     VGVIKSIWNGLVDLVSGVLEPVIDLADSLFKDKVKGVKEAWNKLKDFFKHPIKGTIEI
                     FKKGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNSS
                     NGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKIGGVL"
     gene            27317..27793
                     /locus_tag="ACGI57_000205"
     CDS             27317..27793
                     /locus_tag="ACGI57_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFG9715296.1"
                     /translation="MNKKILVLTISIILSLSLVGCSTNNSKNRDSKSKQNTEEVKNHK
                     IGEVVDLDGAKVSVKNIRKLETNSNDYAVEIEITNTGDNDLQSDYLENYILKSSDNTT
                     GEAINNQELNGELMFDTIRPGDTLKGEIAFELTKDATPKTLEIAYENVGKYTVFDL"
     gene            27851..28486
                     /locus_tag="ACGI57_000206"
     CDS             27851..28486
                     /locus_tag="ACGI57_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFG9715297.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDIYFTLSLKEYRRIQISKVNINNDEKLSSEKNVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYSNGDLWEKIYKANEDEIKDPSVIKNGWVLIIP"
     gene            28727..30244
                     /locus_tag="ACGI57_000207"
     CDS             28727..30244
                     /locus_tag="ACGI57_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9715298.1"
                     /translation="MIIDRSKDSSNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFVDNKFPLGNLPKTNVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESIENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYPAEFTAYCPR
                     KEEGGNTDCRGKKLDPSKKTCAAPMVGAYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNMTEKA
                     KTVISEARKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            30258..30590
                     /locus_tag="ACGI57_000208"
     CDS             30258..30590
                     /locus_tag="ACGI57_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HFG9715299.1"
                     /translation="MADPINEFIGIIRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILIDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGNTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            30583..31017
                     /locus_tag="ACGI57_000209"
     CDS             30583..31017
                     /locus_tag="ACGI57_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFG9715300.1"
                     /translation="MNNTIFPFMGVPEDYISPDNEELPIFKEFAWDFNKDEKIIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVHLIYDWSYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     EALLTNPYILEVNITNAEFEDDNLSISLNVKTIYGEEEITFV"
     gene            31010..32062
                     /locus_tag="ACGI57_000210"
     CDS             31010..32062
                     /locus_tag="ACGI57_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFG9715301.1"
                     /translation="MYNGQSFSTLNNRTLNNINLPLYKGQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVTFEGKIGTQIPNGTII
                     SHSDLLFVIIKDITIDENSKLNVSPIQALEIGIKYNLSANTEFKLIEEITGVTKIYNE
                     LDLKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCTEHIEEEKPIGPTVTIVTPSTFDINISATLTLENGY
                     DIESIKVVFLDIINSYLIENSREIVYIKVMSLLASIEGIHDIKNLLVNGDVKNIIVDE
                     EKVPAVSSAIFDIEVS"
     gene            32064..32672
                     /locus_tag="ACGI57_000211"
     CDS             32064..32672
                     /locus_tag="ACGI57_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFG9715302.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLEQFFID
                     TATWGLDDWENLLSIKVNYKLDFDTRRSNIKAKMRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNNCSYNTILELDKKIEEIKPAHLEHKFERILFNKNELYTGAA
                     ISTGETVTIYPYVPRNLESFGEIAICSGNDRALEKVTLYPKK"
     gene            32689..33471
                     /locus_tag="ACGI57_000212"
     CDS             32689..33471
                     /locus_tag="ACGI57_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9715303.1"
                     /translation="MTEQQYFTLVTDIGKAAIANASITGEKVDFAKIKVGDGGGSSYT
                     PNESQTALKNVVWESTLEHAQGDKDNPNWVVIQKFIPGDVGGFEIREVGLFDSKDQLL
                     AISSYPTTYKPTADSGTVKELLIKVILVVSNVANINLKVDPTVILATLKDIQELDTKI
                     DTTKTELTSNIETAKTELNTKIGDTTQLTTTDKTNIVSALNEVKTSVDSIETTADKTS
                     IKDTDNLFESDNVEGALKEVMQEVKGNRSSIISTINSNLIPM"
     gene            33488..34933
                     /locus_tag="ACGI57_000213"
     CDS             33488..34933
                     /locus_tag="ACGI57_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715304.1"
                     /translation="MKIWKYNRTVKKRRGVYTELDILAQRYEFEADGKLYTAYFHLYT
                     NKNIDIFRDKDLYKESYMMSYRVGNVTTDIDDVVQQTTTVRFIPDPVIDIVIDNISRG
                     CEINYRIIDNEPSVKFIVTEKLNGTIIETKTNSSDNNYKLKLTDEQIVPLAFNSQNSI
                     VIEISTEDGGLVTSKTVTFTRTNNKPTILVNSYSSNSAKFTASDLDNNLSKIEWYLDD
                     VLKETITTDLTAEKTINYEFTDNAIHTLKIVATDAENATVEKVLSISKEIMPLQSDAS
                     LSDISTKLIEIGEGFRNGKTSIINTLALKNIEASLNNTLVELSEKIKTSFDSSDASVQ
                     DLQNRITELNNQLSRRKKWATGRYTFTREEDTINFRMVDGDSLSAVKKLIIPINLDFV
                     PSVIIIDKMFLGSYVPAQDVQFNCVSNLADFTLGRRFYNKQWICTSSIKLDSVTQNSF
                     TLSLQKVVPFDKVSSTIFSIINTGFAWRAYE"
     gene            34957..35304
                     /locus_tag="ACGI57_000214"
     CDS             34957..35304
                     /locus_tag="ACGI57_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715305.1"
                     /translation="MNRNNRIIYDQTGKIWLQTGEAIGDIQELSEITELNFIDVEFGS
                     IDYSKQYIESINPVTKELNIKNIEVVLTDEQKRLEVLEKELNMLKEENKNRDSEIVNT
                     AFEVGNIKLNNNL"
     gene            35316..35486
                     /locus_tag="ACGI57_000215"
     CDS             35316..35486
                     /locus_tag="ACGI57_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715306.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKMDVFYAVNRITEEQYLELTGL
                     LNKEETPVEPTI"
     gene            35516..36085
                     /locus_tag="ACGI57_000216"
     CDS             35516..36085
                     /locus_tag="ACGI57_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715307.1"
                     /translation="MTFKELVSKVRNLVLEAKNVTIEDTENKFTSENVEGALKECIDR
                     ADLAFQSADSGKELIATAIGSPATSEQTFQEYANYIAGFKNTISQLNSKYKYTSGTFT
                     YYTGAYPNGLNIGFRPDIISYTAINAVEEKYYCVYTPLFYLFYNYTTGEKYLDVNHNR
                     YGITFTDNGWIPRNPVNNGVYSYIAAKCR"
     gene            36144..36572
                     /locus_tag="ACGI57_000217"
     CDS             36144..36572
                     /locus_tag="ACGI57_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFG9715308.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGK"
     gene            36576..37406
                     /locus_tag="ACGI57_000218"
     CDS             36576..37406
                     /locus_tag="ACGI57_000218"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9715309.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADIFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPRVNAGGYDLLIELHLNASDRQGKGSEVLYYSN
                     KGLEYATRICKKLGTVFKNRGAKLDKSLYILNSSKPTAILIESFFCDNKEDYEKAKKL
                     GYEGMAKLIVEGVLNKSIESNEVKQMYKHTIVYSGDDRVSADILGLYYKRAKESYLVI
                     DIKDYKPHRTQNLYVIGGVTCNKMKEMSKTTGEKFTQLYGNDVWSTMDKVIEFVKEKL
                     "
     gene            37478..38584
                     /locus_tag="ACGI57_000219"
     CDS             37478..38584
                     /locus_tag="ACGI57_000219"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFG9715310.1"
                     /translation="MLTFLDLFAGIGGFRLAMERAGHKCVGHCEYDKFANLSYKAMHN
                     PKEDEWFEKDIREIRAENIPKADVWCFGFPCQDISIAGNKLGFKGNRSSLFFSVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNRGFDFAKLLIELDEIGYNAEWQNLNSKNFRVPQN
                     RDRIYIVGHLRRRSTREVFPITGTSRKTTLEQLNNPNHSSNRIYNSNGLSKCLLSKGG
                     NNTGLYRVGNINPSGNGAGGQVYSSEGFSPTLTTGKGESPKILIKENTKKGYIKASIG
                     DGIRLDHIEGKTGRGRVQHKKSCTLTTASNAGVIGKKFRVRRLTPKECFRLQGFPDDY
                     YDRAASVCSETQLYKQAGNSVTVDVVEEIAKKLK"
     gene            38791..39057
                     /locus_tag="ACGI57_000220"
     CDS             38791..39057
                     /locus_tag="ACGI57_000220"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9715311.1"
                     /translation="MSKKEIIRICNEKHNWRKSATKTLLKRLVKKRVLLKRIKIFHIY
                     YIPIVTEKEYFKYKINDLEPDYLDEYFIRLMSTVHKFKNLDEED"
     gene            complement(39068..39583)
                     /locus_tag="ACGI57_000221"
     CDS             complement(39068..39583)
                     /locus_tag="ACGI57_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715312.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSEFESLKKVGDELGANESTIRK
                     WLNKKGYKRTGNKFVEDNKSYDDKYHIDSISSKIDDDIKYLSSEIDNIKNVLEWFKTK
                     DDSYHICVIKENDISIDLPDQSIKRTTVRINNTVWEMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(39666..40571)
                     /locus_tag="ACGI57_000222"
     CDS             complement(39666..40571)
                     /locus_tag="ACGI57_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFG9715313.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEIVDLGLGLPVKQFAGLRKNLIEK
                     LQGKEYHVEFTRGNETTKRDITIRSIQTFPEGVTGYLYYAKDIVDQIAGRDVVLVDIG
                     GKTTDIALVQGNKATDPYSINVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYINK
                     GFYLNGEKQDIKKNIDASISLFKEIYNELKLNYPVSTAAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            complement(40591..40794)
                     /locus_tag="ACGI57_000223"
     CDS             complement(40591..40794)
                     /locus_tag="ACGI57_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715314.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMQEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
     gene            complement(41043..41501)
                     /locus_tag="ACGI57_000224"
     CDS             complement(41043..41501)
                     /locus_tag="ACGI57_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715315.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSVVVPGGTANLTAMSGGQLSWRVTSTLGNILGFEGNIDVYKAGKIVSSQYVG
                     FRLAGTTKTGVVTFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
     gene            complement(41536..41838)
                     /locus_tag="ACGI57_000225"
     CDS             complement(41536..41838)
                     /locus_tag="ACGI57_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715316.1"
                     /translation="MEVNLHTGKDEFFKSHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            complement(42226..42378)
                     /locus_tag="ACGI57_000226"
     CDS             complement(42226..42378)
                     /locus_tag="ACGI57_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715317.1"
                     /translation="MDNFLFNVLASLTASVVVYLISELFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            complement(42498..43232)
                     /locus_tag="ACGI57_000227"
     CDS             complement(42498..43232)
                     /locus_tag="ACGI57_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFG9715318.1"
                     /translation="MYYVYKYINLDTEECLYVGKTENICDRHASHLSNKKENWCNKNL
                     RFEYIELDNKYTMDFYEIYLINKLEPKFNITGKGEMDIAKTSFSYNGQWVIYSERDLM
                     SSLASKRCGINYEVNAKMLGIMRKLKKISSNEEIFIGNENIRIKYYFETVLEGINLEP
                     CILSVAYKTLKESTVGTVISVKREYLSENVCLIIDSIFLNEIMKDPLMSKSDIKDLNS
                     QVNDILKIIGVDCEWNKLSDYCSANS"
     gene            complement(43252..44439)
                     /locus_tag="ACGI57_000228"
     CDS             complement(43252..44439)
                     /locus_tag="ACGI57_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFG9715319.1"
                     /translation="MEKKEILMQPNNLIKSKYDFTNVENKLFYKILFNAQKQQNSSYV
                     TTISKEELKVFMKNNNDYEHKNIKEILNMFQQSVLEFDYIEETTGKLKTFGSGLINTY
                     ELDHTDQIYTIMMHEVLYNHITDFVKMQKKKNGYTAINLSVLFNFRGAYTQRLYTLFR
                     LWSRENKEVEIKYKLDELRFYLKLKDNVYPEYKYFKQNVLKRAMNEINKKGNMVVSIK
                     EEKRKNRKIDEIIFSVIDYEPRKYFDKDILVEDYIPKKKQDNEVSFSFYIPNENIFTT
                     GTIKLFKEDFKEFDFKKDVYLNAFNKSIAIAFERDNTDIINTKNYDFFKSTLSNKISE
                     VIIHQKDDLKFKEELDKYWDDEDVKKEEREVDYSKDPVRLETVRRNLFDQGLVNEEGY
                     YYK"
     gene            44859..45548
                     /locus_tag="ACGI57_000229"
     CDS             44859..45548
                     /locus_tag="ACGI57_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFG9715320.1"
                     /translation="MLENRKQIQKEIYLPIATYCLKDKQYYIKEYGALLIKSAENSDI
                     FNTHKNRILYKNKLDIQEIANELNVNKATIQRNIKKLEKLDFKILKIENTLNGIVYCL
                     PSENNIDLNKFALINYEMLKKMVNKFKSNTIKVYFLLKTITTETNFKPATNSFIAENI
                     GLSSKSKNNLDIITSSVKTLEENKYIETKKVNVYEYDKEKLREVPKIRKVYRICNFDE
                     WKKEIDKNKVY"
     gene            complement(45697..46080)
                     /locus_tag="ACGI57_000230"
     CDS             complement(45697..46080)
                     /locus_tag="ACGI57_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9715321.1"
                     /translation="MKIKKIPESELLVMMFIWDSEDKEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALIEKEEYLKVETKSFFSFFHKNSFESFISAL
                     HDDEEVSENKLKSLEKFIEDWEEDK"
BASE COUNT        18543 a         5380 c         7705 g        14564 t
ORIGIN      
        1 aaaaaaaaat taagcaggag taaaaaaaat aaaatattat caaaatttat caatataaaa
       61 agaaatataa gtataaattt ttctctttgt atattgattg tatacaattc tatttttgta
      121 aaattaaatt ttaaaaaata atcaaaaatc ttaattttca atattgaata caattaaatt
      181 ttcaaactca tctagggcat gacatacaaa agcaaaaaac attaatttag tatctgataa
      241 aaatttaaaa attgtaaaag ggggaagtct gttatttttc agaagcaatg taattgtaaa
      301 aatataaatc agaatatttt gaaaatatgg gggataaact tatgatggat ttagaaaagg
      361 aaaaacatga aataattgaa attataaaaa aattaaatat tgataaagat aaaaggaatt
      421 tattgatgat aaaaaattat atccttggac ttttaaaaaa atagaagaat agcaattgct
      481 attcttctat ttctagtaag gaatctatca ttttatcaaa tacagtcaga tattttttat
      541 tcaatttgaa cattttagtt ataatttctc tttctacttc attttccatt ttacttagtt
      601 caccaacaag atgggcaaat tgctcatcta attctttgat aaaaaacatc tctccaactc
      661 catgcttaag ccattcttca gatatgctcc aagtcttgca aatgtcgttc aaagttcttt
      721 cgcttgcatt tcttttacca ttttcaatta aagatattgt gtttcttttt aaatttagtt
      781 tattagcaaa ctcttcttgg cttaaattga gatttaatct tagttctctt attcttttgt
      841 tttctggttt catttcatct atcattatta tccctccata tgaaatatta tattttaaaa
      901 gtgcttacaa gtcaacaaaa ataaaagaaa aaggtattac aaaatagttg acttgtaagc
      961 agacatgtaa tataattgta ttacacaatc aacaaaagcg aggtgattga aaatgactga
     1021 tataaataaa attagagaag aaatatatag aaaattggag ttacttggcc ctgcaaaact
     1081 aaaattagca ttgactaaag tagatatatt aaaagaaata caagacattg aagatgaaaa
     1141 taatatacaa aattaaaggg gtgaaaagat tgaaaatctc agattttaat aaaaatcaaa
     1201 taggatcagc agttcaatta caagatataa ggtttgatac aaaagctgat tgcatcatta
     1261 cgaaagtaga aaagaatgaa atactagtca tgtattatga aaaagaaaca gaggaaatag
     1321 catataaaac tttgactaaa gaagatctaa tacttgatga ctataaactt aagttattat
     1381 cttaaagtaa tttcagaagg gaggtgataa ctttggatct tttgaaggat gcaagaaaaa
     1441 aagagtggtt ttggctggaa aatgatttga ttgatagaga agatttgagc atctatgaaa
     1501 aaatgattta cattgtctta gctagatata gtaacgaaga aagttcttgt ttcccaagtt
     1561 ataaaacaat atcagaaaaa gcaggttgca gtataaggca agtatctaat attttgaatg
     1621 aattagagaa taaggaacta ataataagag aaaatcgtaa ccacgaaggc aaaaaagaga
     1681 aaaacagtaa cttatatttc ttaatatctt caaaagcaaa ggtacagaat gaagtgccta
     1741 acgttaggca tgaggtgcct aaccctatgg caccaggtgc cttacatgtt aggcatgagg
     1801 tgcctagtaa aaagacttat aataaaaaga cttatataaa aagtaatacc actacacaaa
     1861 aagaatctaa aactgattat ttagacctat cattcttgga cttagatata gaaaaagtaa
     1921 aactaactaa agatgaatat gacaaactta taagcaagtt tgggaagaaa tacatacacg
     1981 ataaaattgt tagtttagaa aactatatca taaatggtaa agggagtagg tataagagcc
     2041 actatagggc tttgctgact tgggggaatg gagatgctag taaaggtata gtaccagaag
     2101 ttacaaagac taagaatcct cttagtggat ttaaagaact ttaaaaacta aatagtaagg
     2161 aggtagatag gatgaatgaa aaagaagaga ctgtttattt gaatagttct aatgtgaagt
     2221 tattcttagg cgttagggat attaaggaat ttgaggaatt gatagaaaat gtcaatgagc
     2281 gaatttgtca actccaagaa tcgattaata aacttgccca atttaatatt gagttcgata
     2341 ttaagtcaga tatttagtta agtataaatt aggaggttgc ctatgaaaga tgaaaagtta
     2401 gaacttactg aaaaagattt acactgtatt gcaagacatt tacaaaatga agttatggag
     2461 atggtgttta gagggaatat agaagcccct acatcatgcg aagtttgcga ttacttgcaa
     2521 gaatgcaagg actatttcac ccatatagac acttttataa aattaagtga aatgacaggt
     2581 gtagatattt ttactaaata aatttagtaa attaaaattt tagtattgta attgacgtgc
     2641 ttttttaaaa agaggacagt tgctattagc agtcattatt tgaattttgc aaatgtaatt
     2701 tttattacat tcgcattcta aaagttttga tccagtgaat ccaggaaaag ttttatattc
     2761 accagaaaat tcaactttat cgtcaataag agagcatttt tctcttaagg ttttaatttt
     2821 gcacatattc atcaccaccc tataattaat ttgaacatac tgtcaatatg ctccgattaa
     2881 ttataacatg caaattatca tgatgtagaa ttaaagcata ggtcaattaa atttagttat
     2941 aaaatcaatg aaacagataa ataattaaat gttaaataaa ttagggggtt gttcatgaaa
     3001 gaggaaaagt tagaacttac tgaaaaagaa ttgcattgta ttgcaagaca tttgcaaaat
     3061 gaagttatgg agatggtgtt tagagggaat atagaagccc ccacatcatg tgaagtttgc
     3121 gattacctgc aagaatgtga gggagatttt acctgtatgt atcattcttt tactaaatta
     3181 agcaaaataa caggtgtaaa agtgactgca tttaagtatt gttttccaga tgaaaatccc
     3241 aaagctacta tctaagaagt ttttttagaa taggacattc ttcattagaa gaagagtcgc
     3301 aaatatgact tccatcacat ctagttgaca ttaacttaga tccaaaagta cctggaggag
     3361 ctgaatattc gcaagtaaga gaaatggttt cgtttattac aggacattct ttgcttataa
     3421 ctcgatattg gctcatactc tcaccacctt tctaattaat ttaaacatat tggaagtatg
     3481 tttaaattaa ttatagcatg taaattatca tggtgtagaa tagtttcaaa tcaaaagaag
     3541 caactaaata gttaaagaca ggaggtatgc taagtggaaa gaaaaaaaca tgaatttaca
     3601 gatcaagata tacaaagact tattgaagga ttaagaactg atccaaaaat aagagaaatg
     3661 atatttaaaa gtaaaggttg tgaaactaga acaagtacaa gaagtaaatg cgaacaattt
     3721 gttgaagaaa aacttgctta tagaagttat ggatttgaaa ctccaaaata ctatgcaaaa
     3781 cttattgccg acataattga tattttagtt tacaaaaatg ttccaagaga tcgtatttgt
     3841 ggggtattgt atgacgtcga aagggtgatt cctttagttg tgacatttta ataataagct
     3901 gggggtgatt aaatgaatta ctctttagaa gcagagcaaa acattctagg aagttttctt
     3961 ataaatgact ctataggtta caaaataagg gaattaaaag aaaatgattt ctactttgag
     4021 tcacataaaa ttattttatc ttgcatgaag aaaataattg actcaaataa accactagat
     4081 cttttgcttt taaaaaatga attagaaaaa atagatagat tagtagatat tggaggcgtt
     4141 agttacatta ccagtttaac aactatagtt ataactactt caaacataga tcactatata
     4201 aaaatcataa aagagaagtt acttaaaaga caaatttcag agttagcaaa tgaattaatt
     4261 aataattcaa attcagatac aagtatagat gaattgatta tagatataaa cgatttaaaa
     4321 gcacttgtga cgacgagcag tagtgtaaat gataattaca ttgacgcatc aaaaataaaa
     4381 cgtgaaaaag gcgtacacaa gtccatagac acgggtttta ataaactaaa caatatgcta
     4441 gatggattta gatatggaac tcttacaatt ttaactggta aacctgcatc tggtaaatca
     4501 actatagtta accagtttat agcacaagca attacgaatg gagagaaagc atttttgtat
     4561 tctggagaat taccctcttt catgtctatg gattggttta gaaaaactgt agctaatgat
     4621 tatcacataa aagaatataa aagtgtctat ggaggaacat acacagatat tccagattac
     4681 gccgttgagc ttatatcaga ctggatagaa gataaatttt ttctctatga tgaagatgca
     4741 ataagtgatg aagtaaatct actaaatacc atagagcatt tatatttaaa aaaaggggtt
     4801 aggttctttg ttcttgacaa tctaatgaca ataaaaactg gcaataaggc agataaatat
     4861 gagagacaag agcagatagt aagcaactta aaaaatctcg ctaaaaaata taacttagta
     4921 atagttttag ttgcacatcc tcgtaaaaat atgggtgaca tgaagccaac tatgtatgat
     4981 gtgtctggag caagtgagat tgttaattat gcagattata ttttatcaac ttacagagtt
     5041 gttgatgaag aagaagagac agacgacact tatttattaa ttttaaaaaa tagaataaca
     5101 ggaaaacaga atataagttt taaaatgaat ttttcagaaa gaagaaaaag actttacacg
     5161 actagtgagg agttaaacag agattacaaa tatgatgtaa ataaacaata tgttcaagta
     5221 gaaattccag aagatgtatt ttaaaaaaag tataaaaaat aggaaatttt attaagtaat
     5281 gattttataa gaaaaaccaa tattgaataa tttaaaaatt caatattgaa aaaaatcatt
     5341 tttcaatatt gaaaaacgga ttttgaaaaa gtattgaatt tcaatactaa aaaaagtttg
     5401 aaaatatagc atttacaagg tttatataaa ttaatttcct gacaatgtac acacaggttt
     5461 agatatgcta gcaaaaaaac ttaaaagtag tgtaaggata aaaaggctat ataaatttat
     5521 aaaaagattt aaaagtgtta ttaaattaga gctaaagttt attaattaat ttcctcgtag
     5581 aaaaatatta ttttaaataa ataaaggagt gattttacat gatagcatta gaaaaatttg
     5641 caggtggagt tctaaaagaa aagtttaata cagagttaca aaaagtttta gataacattg
     5701 cagatccaaa tacagatttt aaaaaaacaa gaaaaattag tttagagata gtgtttaaag
     5761 caaatgaaga tagagatctt gctgaagtag atataaaatc caaagcaact atagtagaag
     5821 caaaggctac aacaactaaa gttctgatag gaaaagattt agagacagga agagttgaag
     5881 cgtctgaatt taaaaatcaa gtagcagggc agttggtcat ggatattcca gatagtgaag
     5941 aaactaatga aacagaagaa aatagtgctg gtgtaattga tttcagaaat gcaagttcaa
     6001 agtagtttaa gaaagtaagt aaaatataaa tttaaataaa aaaggagaat aaaaaaaatg
     6061 ataagaaatg caatggaata tttagttaag ttaggaaaaa gagaaacagt atgtgtagat
     6121 gggtttaagt acacaacaga tgcattagaa ataataaaag aaccaaaagc taatgaatta
     6181 gaaataacta cattgagtgg tttagtagat tatataaaaa gtggcataga tcataaggca
     6241 gatgagttgt tattaataca agttctaact ccaagaaaag tgcttttaaa atctcctttg
     6301 agaaaaaata gagatagaga gacatatatt gaatgtgttg cactactacc agagaaaaga
     6361 tttgacagac ctttagactt agatagtttt aacataatgc tacaatcagc atttataaag
     6421 aataatgata gagatacact tttaaaaatt gcaggaaata tccaagaatc tacagtaaaa
     6481 aatgtgggag atgatggagt ttctcagtct gtaacaataa aaacaggcgt agctagtgta
     6541 tcagaagcaa tagtcccaaa cagagtaaag ttaattcctt atcgcacttt ccaagagata
     6601 gagcaaccag aaagtgagtt tattttcaga atgagttctg gaccagcagc aggattatat
     6661 gaagcagatg gtggagcatg gaaaaatcaa gcaatgttaa acattaaagc gtacttgcaa
     6721 gaagaattaa aagattttaa aaatgtaaat ataattgctt aattgaaaat ctaagagggc
     6781 ttacaataag tcctcttagt aaaaaaggag gggattagat gaaaatgatt tctcatatga
     6841 acattttaga aagagcagaa tttacagata aaatagctaa tgcaatcatg tctgcaaata
     6901 aagaaattga aagaggctgt gcctttaatg aagcagttga gattgtaaaa caaatggaat
     6961 atagagaaaa taaatacaat gaaggagata ttgcaatatg aataatgtag ttttagttgg
     7021 aagattagca agagatccag aattaagata tataccagag tatggtactc cagttgctac
     7081 ttttgcattg gcagttgata ggggttatac aaaaaaggat ggaactagag aagtcgattt
     7141 tataccaatt gaagttatgg gaggatccgc agaattttgt gctaattatc tcgcaaaagg
     7201 aagaatggtt tcaattcaag gccaaataag gatagaaaaa tatgaaaaag atggagagaa
     7261 aaaaactttc acaaaagtaa gaacaaaaat tgttaatgcg cttgatcgca agccaaagga
     7321 tgaagaaaaa gaaattagtt ttcaagtttt agatgatgaa gatattccat tttaaattgg
     7381 atggtgatta tgtgagaagt ttagaggaga gaaataaatt agttgaagaa aatataaatt
     7441 ttgtatggtt tattataaga agatatttca aagtatttat ttcaaaatat ccatatcttc
     7501 aagatgatac attttcagaa ggatgtattg gattatgtaa agcagcagat tattttgata
     7561 gtaaaaaagg tattgaattt accacttttg cagctgtgtg tataaaaggt caaatattta
     7621 aatttataag taggtatgtt aaaaaacatt acagagcaga tatagatagt tttgaaaaat
     7681 gcatctataa aaatactcat ggtgaagaaa taagaataga agatagattg gctggtaatg
     7741 aaaatgtaga tatagaaaat gttcatgtta ttagatcttg tataaaaaga agtaaaatag
     7801 aagatataca gaaaataaca tacttaaggg aaaaaggata cactcaaaaa gaaataggca
     7861 aaatacttag gactaaccaa atgacaatat gcaggaagct tggaaaatta aaatctgaaa
     7921 ttaatatact aggaaaataa tataataatt taagttcatt ggaggcaaaa tgaagactaa
     7981 aataattggt ggtagggaaa atcagtgccc actttgtaat ggtagtgtat ttaagattga
     8041 aacatcaatt ggaatagtat ggcaatgtaa ggattgtgga tgtatgtatc aagatatgaa
     8101 ttgtaaagaa aacaaaagga ggcattaagt atggcttgtg ttataaagtg tgatttttgt
     8161 ggtgaaacaa tagcagaaat tctaaacata aaggtattca gaattgatga tgaaggtaat
     8221 ataatgagta tcggaaaaga tgtatgtgac aaatgttatg acagattatt tagaagtaac
     8281 ttgaataaga agatgactaa ttatgaaaag ataaaaaata tgagcaaaat agaaatggct
     8341 gaatttcttg caaatggcgg aagtggatgt actagttgtg cttatgattt tcaagaatgc
     8401 ttaggcggat gtttagaagg tagaaaaaaa tggcttgaaa gtgaggcgat gaaataatga
     8461 aaaagacgtt aataatcgta ggagtagtta tttcaatatt aattatgttt gcttgcttgt
     8521 tcttagtaac taaaaataaa gcaattgatt tagaagagca ggttcaatca gcaactgcat
     8581 ctgtagaggt gcaagaaaaa aggagaattg atttaattaa gaacatagta gactgtgtaa
     8641 aagactatgc taaatatgaa aatagaacat taaaagaagt aactgacatg agagtaagtg
     8701 caaatgatga atctatcaag gaagcaaaat taaatataca agcagtagca gagaagtacc
     8761 cagatttaaa agctaatgaa aattataagc aactaatgag tgagttgagt ataacagaaa
     8821 atatgattgc acaacataga gaaaattata atatgcaagt tcgcaattac aataaatata
     8881 taagaaaatt tccagcaaat ttaattctaa atagtatggg atatgaaaaa atagaatcta
     8941 aatatactga atataaagca aaagaggatg cacctcaaaa tttatttgat taagaggtgc
     9001 acatatgaaa tttaaaactt ttgaaataac taagagagaa tttttagtga gcataataat
     9061 tgcttgcatg atgattgtgc ttggattttt cattgatgat aaagttcaat caaaattttt
     9121 agaagataat gaaagatatt acaaagcaac taaaataaat aatgacaaag atctctttga
     9181 ttatgctatg gaaacaagtg cgggagattc tttgataagt ggaagattta aaagtgtaga
     9241 ttctgtatct atagaagaat taaaagggga ctattggttt atagaaagaa cggaagaaaa
     9301 atacactatg catactagag tggttaccac taaagacagt aaaggccatt cacataaata
     9361 tactgaaaca tattggactt gggattacca tgattcaata gagaaaaaat gtaataaatt
     9421 taatttcatg ggtaaagagt ttgatgcgaa agtaattact aatttatcta aagagagatt
     9481 ggcattaaag aaagatatat ttaaaaataa taatcttaaa ataaaaggta attatgctta
     9541 cattaataat aagagaaggt actattatga agttataccg aaagaattta atggtactat
     9601 gtatgctaat ttaaaaaata atactatagc gaatccagct gataaaaaca taaaagtttt
     9661 taagggtgag tctatacaga atgttttaga aaataataat tttaatacag ggttttttaa
     9721 agtagtgttt tggatcatct ggatattaat aactacaatc attatatatg tattttatta
     9781 tttaaaaaat gattggcttg aagataaata aagatgtaag aggagaagat caggtatgaa
     9841 atgtaaggtg taccatgcta ttaaaaatat aaaagatgca aatgagttaa ttgatagatt
     9901 tgaaacggaa aatatattct ttgaattaaa tgacatagat aatgaaaatg tcttaaatgg
     9961 agaaatgaat ttaactgata tagaagatat tgcatcattg caatttatat tagatggaga
    10021 tttatttata tttagtagtt gtgatgaaat gatatttatg aataaataca ctcaaaaaga
    10081 gttgggactt caataaatga atgaggggag atactaaaga tggatttaaa gttaattagc
    10141 agtataccta attggaaggt agaagatcag ttggcaaaaa ttgatgaaga ggtaactgag
    10201 tttaaaaatg ctatagatac aggaataact aaagatataa ttgcagaagg attagatgtt
    10261 tgtcaaacta ttttaacaat gtttcaagtt ttagaaatag aaaactatat tactgaagga
    10321 gtagagacac ataataagaa actaaatgga agaggatggg atctcaaaga aataggagac
    10381 gatatactta atttaattaa aaattcaatg attaatgaat tgattaatat gaacaagaaa
    10441 ttaaaatgcc ttgatgaaaa agaatttaat gcaaaaatac aatttttaga tgtagataaa
    10501 ctatggaatt tgtgcatgcc tttagttaac ttaagtacta taaaaggagt taatgaaagc
    10561 attgatgaaa tgaaaatggg agagtttcta aaaaatatta attaaagtaa ggagataata
    10621 taatatgttg atagaagtaa ctgaaaataa tagagaatta aaagtaggag atattgttga
    10681 aattaatggt ggtaataagt tatatatgat tctttcttta aaggaaacaa taggtgggta
    10741 tgttattata aatatgcaga atggatatgg ttcatttggt gcttataaaa gtttaagtca
    10801 actagaatta gacttgaagg taagaggtta taaattatat agttctgatg attacaagtt
    10861 gcaattagtg cctagagaat aagatatatt tcaaaaaaga aaaaaaggag cattacttca
    10921 cgctcctgct tgtcaaaatt ctaaaaccgt catcacaaca tcattataac ataattagga
    10981 gtgtgaggta atgcaatcta gtaaaaaaga caatttgttt agtagtgcag aaggtaagtt
    11041 atatgattat aaaaaaataa aagcggaaat agaaaaaata gacattgata tgcagatgat
    11101 aaaaaacgac tacagaggat gtagtggagt tgaaattaaa gaaaagacag gcaagacata
    11161 tgatattaaa agtgtaattg aaatagagac tgaagaaaaa gaaaagaaaa ttgccttaaa
    11221 agaaaaagag aagagacata aagagttgat aatagaaaaa atagataatg caatgaaaat
    11281 attaagtgaa gaagaaaaaa agatagtaca gtataaatac ttttcaagta gcaggacatc
    11341 ttgggaatat gttggcagaa tgattggttt ttcagcgagt aagtgtaagc aaatgaggtt
    11401 tgatattata gataagataa aaggattgct ataaaaatgt ccgatttgtg acgattttaa
    11461 accgatttat gtctgttttg tgtctaaaaa atgtccggtt tgtgaccaat ttatgtctgt
    11521 tttgtgtctt ttaatcatga taagattgta ttgtgagaaa aatatattta ataaaaatgg
    11581 cttggaattt acgttctaag tcttttttat tgtctagaga ggtgttgaag attaaacgtg
    11641 atataacaga agaaatttca agtgctgcat atgtaccaga caatctaaag tattacgaca
    11701 atgtaatgaa agaaactcct ggtatgtatg gaagagaatg ttctttagat tttataaaga
    11761 aaaagcaaga aaaactcttg aaactaaaaa agaaggttaa gaaaaattat gattctaata
    11821 ttaataagat agactcatat ctaaaaattt tagagaaaag tataattgat gagtctgatc
    11881 acgttgagtt ggttgtattt aaattatatt tgcaattaga aaatgttgta aaagtaactg
    11941 aaactgtaaa cgacttagga tttagaataa agactaacac ttatgcaaga gaaagaaaat
    12001 atagtacaaa tgatataacc tctataatca cagatccttt tactgatgta gttgaggact
    12061 taaaaatatt agttcaagag aggcaaagaa aaaattatta tggaaataag gagtgttttt
    12121 aatgaatgga agcacatagt ggacaaaagg ttaatcccat aaaagatata gaggatgtat
    12181 ttagactttt aaattttcta gaagaatgga atgaaagaaa ctatttatta gccctctttg
    12241 gaatgtgtac tggacttagg gtaggggata tattagcact taaagttgca gatgtaacag
    12301 acttaaaact cgataaaaaa ggaaataaaa taagagtatc taaggattgg attagagtta
    12361 tcgaagaaaa aagagactat aatagagagg tttttttatc tgatgtaata aaaagtgcta
    12421 tagaaaatta tactcaagat aaaccaggag aagaattttt atttaaaagt ggcaagagga
    12481 agaaatttaa tagacctatc caaacaagac aagcaggaag aataataaaa ggagcagcaa
    12541 aaaaggtagg aataaaagaa aatgttgcaa cacactcttt aagaaaaaca tttgcaaggc
    12601 atatttatga tgaagaagag aataagactt atgcattaga acttataagg aagattctag
    12661 gacataagac aatagaaatg actagaagat atataggaat agataaggat gaagaagtaa
    12721 aagcaataac taaatttact aataagttaa agaaaagaaa aagaaattaa aatatcttgt
    12781 attttatcta aaatttaata tgttacgttt ttcaacaaca ggacattatt agaatgcaaa
    12841 gagccttaac aatagagttt acaaggaata gaatagattt attaaaatgt ctgaaaatat
    12901 atattaaagg tcatttattc aagggattta tagggatgta gatatttaaa tgtttattta
    12961 gaagctgagt aaagaggtga ttaattggat gtgaatgaag ttgaaataat cgatagtagc
    13021 tatgaaaaag gtaaagagtt gacagaagat cagtgcaata tggtaactct attgattaca
    13081 ggtgctacag taacagaaac tgccagaatt attggagtaa atagaaaaac aatctataat
    13141 tggatgaata aagagcatgt taggaaagaa atggacagac ggaaacagga gctaacaaac
    13201 caaggaaatt taatgatatt aaaggattta gatagttata tcaacaatat caaggaatta
    13261 gctagtgaca agagcgataa gagggttatg ttggcagcca atcagtatct tataaatcgt
    13321 atatatggaa ctcctacaag tacagttata caagcagagg ataatagtgt tggcatgggt
    13381 atggatgcaa cagagataga agcagctata gctaagataa gaataagaac aagcaagaag
    13441 taattagata tgaaataaat cctgcaagaa tatgttttaa ttatgtttga aatcaattaa
    13501 agcaaaacat atattatata tatatttact ttaataatta aatcatatat tgtatttaac
    13561 tttagttata gctaatttaa taaaatttag tgagacttaa taattaaagt ttcatacagt
    13621 gaagtgatgt ggattattat aggatatgtg aacctattgg aaatgctagg ttctttttaa
    13681 ctatgccgaa aatacatatt taaggaatag ttgaaagata attaataatt ctaatcaaaa
    13741 aaatatttct tgcaagaaaa taattcaaaa tactttaata tatttatttt ttttattaat
    13801 agggggtgtc cttctatttt gaaaaaatta ttttttatgg aaaatgagtt ctagaaattt
    13861 tctagcaaaa tttaaaaacc agagatgtat ttcaaagagg gtgattttat tagagaaaaa
    13921 tttacattgt ttattttatt aattgcatta ctcactgagc ctaaaaaaat agaagaagta
    13981 agaggaagta aatacgagtg gataatctag aaagaaatat acaattaata tttagttatc
    14041 tagcaaagac ttttataaga aatggagcat ctataaaaga tgctgaaaaa gaagcaaatg
    14101 agattataaa agccaattca aacaatcttt ggggagttaa tggtctcgct tatcaacttg
    14161 gaaaaataaa cctagaattt ttctgtatgt attttatgca agacacatat ttaccaaaag
    14221 aagataatgc tgctgctcct atagctaaag ttcatcatga gctatgggaa gatatacaag
    14281 aatctataat tggagatggt tcacagcaat taggaagaat ttatccaaga gggacaggta
    14341 aaagtgcatt tggagattta gcgacaactg tatggtcgca ttgctataaa cataagacat
    14401 atactttgat ttgctctgac ataggatcta cagcagagaa atttgtaaaa gatataaaaa
    14461 atgcattact tgaaaatgag tatattaaaa aagcatttgg tgtcctttta aatgataatg
    14521 atagaaaata catttgtaat agtactcaac tagagctaac aaataaaact tttattgaag
    14581 ctatatcatc ttcatcaccc atgagaggaa gaaaatacaa taataaccgt ccagatctta
    14641 tcatacttga tgattatcag tcagaagaaa atgttagaac agaagatgct agagagaaaa
    14701 aatttaaaag attttctgat gatgtaaaat atgctgctca aaaaccagtc ataagaaatg
    14761 gtaaaactat aaaaagagga acaactttta tagcattagg aaccttacaa cacaaagaat
    14821 gtttttatag tagactaaaa aatttaccaa cttggaagtt taaatgcgaa aaaggagttt
    14881 tagttgataa tgtagatgaa ttatttaatt ctggtttatg gcttaagttc aaagagatct
    14941 tatttgattt taaaaataca aatcatcttg aagatgctaa agaattttac tgggaaaatg
    15001 aaaaagaaat gaaattccct atattatggc caagtttttg ggattgttta gatatggctc
    15061 taagttatta tgaaaatcca acatctttta agcaggaagt gcaaaatgat gtcaattcta
    15121 taggagaaaa atggtttaaa actgttagaa cagaaactag agaagaaatt gaatcgcata
    15181 cttttaaaaa gacaatgctg ttatgtgatc cagcatctgc gggtgggtcg aaacatgact
    15241 atagcgcttt tcttgttgga agtgaatcag aaaatggttt actatatgga agattggcag
    15301 aactagctaa aataaatgct agaactgatt ttgataaata tatagaccat atgatttatt
    15361 tattaaaagt gtattcagat ataacacata tttatataga aaaaaataca tttaatggcg
    15421 cagatgccaa ccaattagag tttaaaataa aaaatgataa tgttcttagt tatagagata
    15481 tagaaatcat taatgagcaa caaaagaaaa ataaagatga taaaatctct actttaatac
    15541 ctgttttaag caaaggccaa atgatttttg cagaagaaga taaggaattt atacagcaaa
    15601 tacttgattt tacaggacaa aaatactctc tacatgatga tgctccggat atatcatcgg
    15661 aatttattaa tcgaattaac aacataaaag tttctgagac agtaaatatc tttgatagaa
    15721 aattattagg tttttagttg ggaggtaatt tattggctaa tagatcaaag tttgaagaag
    15781 ttcaaaaaac tttaactaaa aaaggaaaga agttcaatgt tggaatagga aaagatgaaa
    15841 aaggatattt tgcatatact catagagcaa ggagtaaaag ttatatttca aagcaagata
    15901 ttccaataaa agttttaaaa tttatagagt ctacaggata ggatgtgttg ttaatggaat
    15961 tagatttaga cttaataaaa gacatatatg acgattggaa ctctaataaa tccgaatatg
    16021 atactatgta taagtattat aaaggtgaaa cggatgctat tagcaactat aaaatggtta
    16081 ccaagagatc taataataag ataaacacta atttcttaaa aaagtttatt aatgaagaag
    16141 tcgcttattc tcttgcaaat aagattacct atacaagtag atctggtgat gagaagatta
    16201 taaatgattt agaatactat acttgccatt ggagtaaaaa acatgactca gatttattga
    16261 ggtatgcact attatttggg ctttgctatg aactttacta tgtaaaagat gatgaaatac
    16321 aagtaaagat aatcaagcca acagatggct gtcattatga aaatgaaaat ggagaaatag
    16381 tatgctttat tagagagttt accaaaggtt tcgaagatga cacgtatgta gatgtatatg
    16441 acaaagaata tatatatcat tttgattcaa atttcaaaga agttgaaagt cctacagtaa
    16501 acaatatatt tgatggaaat gttccaatat caatttgcaa aagaagtgaa gaactaggaa
    16561 aaaatacaat atttaatgat ataaaagggt tacaagatgc ttatgaaact aatttaagtg
    16621 atataagtaa tgaaattagt gattttagaa atgcttattt aaccttttct ggatgtaata
    16681 ttaaagaaga tgatttgcca aggatgaaag aacttggaat acttcaagtt aatggagatg
    16741 gaaaaataga gtggcttatt aaagatatga atgatacatt tgttcaaaat atactatctt
    16801 caataaaaga aaatatgtat gaaattacat ctcatataaa tcacaatgaa aaaatgcaaa
    16861 gtaatacatc aagtctagca gttatagcaa gattaatcag tacagaatgg atttgtagcc
    16921 aaaataatga tagtattgca gatactttat ttaataggta caaattattg tgcatttggc
    16981 ttaataaaaa atatggtttt gactatgatt ataaagatat taaggctaaa tttaccccta
    17041 agataccacg tgatgattta gttgtagcaa atatattaag ccaacttgga gataaattat
    17101 ctactgaaac tggcttatct caactaagtt tcattgataa tcctcatgct gaaatagaaa
    17161 aagctaaaaa agaacaggaa atagtttcag agggagagat tttattagat gaatcaaaag
    17221 attataacta aattgactga agatatatat aaatctgctg aaaataggac aaaaccattt
    17281 tataagtatc aaagagaaaa tagggataaa cttttattag aaatagcaaa gatattgcta
    17341 acacacgata ttattaatga atacttaaat attaataata aagataagaa aaatctaagg
    17401 tcaaaactta ataaaattat aaatgagtat tctaatagtt atgatgaaga agtcaaggat
    17461 atagaaacta ttttatctga ttcatctaag aataaatatt caaatttgat aattttatta
    17521 ggagtaatac tgaagataaa agatgttagt aatataaaca ataaagttat aaataagata
    17581 gtgaataata aggttgatgg aaaacattgg agcgatagac tctggaagaa taaaaaaggt
    17641 atagaagagt ccttaaaaaa agaaataaag agttttctaa atggaaaaac ttctgtaaat
    17701 aagatagaaa aagcaataag agataaatat tcaacaggtg caggacaaac cagcagacta
    17761 atagaaaatg aagttgccag atgtcaaagt gaagtaaatg agtatttttt caaggttcaa
    17821 gggattataa aagtaatgta ttgtgcagat ttagattctc gcacatgtag tgattgttct
    17881 atgcataatg gagtgatatt ttatgtcaat gaagataggc cctctttgcc aagacactgt
    17941 cactgtagat gtcaatatat acctgtttaa catgtattaa ttaatttaat aggtgttttt
    18001 attatgtcta aaaaatatga ggtgaaatat gaaagttgaa atattaggaa cagaatatct
    18061 agtgatagag aaacttgaaa gcgaagatgt gctattaaag gaaagagctg gatattgtga
    18121 tcactctgtt aaggaaattg ttatagagaa aataaattct gaagaaggtt cattgaaaga
    18181 tttaagtgta tataaaaatg aagttgctag gcatgaaata attcatgcat ttcttagtga
    18241 aagtggattg aagagttgta gttcttgggc tacaaacgaa gaaatgattg acttctttgc
    18301 aattcaattt cctaaaatag taaaggttat ggataaaata gaatgtttga attaaatttt
    18361 caaaaaggag aactttatac atgttaaaaa aagaagtgtt ggaattacta aaagaaatag
    18421 aagatgatgc agaaatagat tcattgctgc aatcaacaga tttattcaaa tctgcatctg
    18481 ataaaaagct tacaatagaa gagtttagag aattaattaa taatgatgca aactttaaag
    18541 ctattataga taatgaaaaa aataaatatc acagcgaagc acttgaaaac tttaagaaaa
    18601 aagatatgca gacacttata agtgctgaag ttctaaaaag aactggagca aatgaaacag
    18661 aagagcaaaa agcaataagg gaattaagag aaagcctaga caaactaaaa aaagaaaagc
    18721 agtacgcaga aaaagtttct aaatataaag atattttagt tgaaaagaaa atacctacaa
    18781 acttaattga atatctatta tctgatgatg atgataaaac aaatgcaaat atagagatat
    18841 ttgaaaattc aatgaagcaa tatgtgcagt caaaggtaga tgaaagaatt aaagatggtt
    18901 catacgttcc acctggcaaa gatagtgcag gttcgctatc agaaattaga aaacaaataa
    18961 agcaagggct aaatagtctt tagtataaaa aaagaaagga tgattttata aatggctaat
    19021 gtattacaat atgtaacact ttttcaacaa gaactagata tgcaggtaac agttggctca
    19081 actactggat ggatggagaa aaatgctggt caagttatat ataatggagg aagagaggta
    19141 aaagtaccaa aagtaacaat ggatggctta ggtgattatg acagatctaa aggatatacg
    19201 caaggcgcag ttacacttga gtacgaaaca atgaaaatgg agatggatag aggtaggact
    19261 tttatgttag attcaatgga tgtaaatgaa agtaacttcg ttataaatgc atcttctgta
    19321 atgggattat ttcaaaagca acatgttata ccagaaatag atgcatatag atactcaaag
    19381 atattttctg aaacagctaa agtagatgag cgtgtaaaat attcatacga agctacagta
    19441 actacaatat taagtgaatt gaaaaatcaa cttgcatcaa tacaagattt atctggagat
    19501 atacctctta ttattagtat gtctagcata attgcaagca tattagaaca atcatctcaa
    19561 cttgatgata ttgaatttgg cgatggagat ataaaaagaa ttgtaaagtc tatagacaat
    19621 gtacctataa taaaagttcc atcaaaaaga atgaaaacag aatacgtatt tagagatggt
    19681 aaaacatctg gacaaacaga gggtgggttt actgaaggag caaaggcttt agatataaac
    19741 tggttaataa ctccagcatc tgctccaatt gcagtatcta agactgataa gccaagaata
    19801 tttactccag atcaaaacca aacagcagat gcttatcaaa tagactatag aaaatatcac
    19861 gatttgtgga tattggatga atatttaaaa ctttgtagag catctgtgag acaagctaaa
    19921 gctgcacaag aataaggagt tgttttaaat gtttgcattg ataaaaaata acataattaa
    19981 agttgttgaa gatgaaaatt cgagagataa attgatttca aaaggattta aaatatattc
    20041 agaagaagtg actaaagaga atattgagaa aaaagaagaa aaagttaaat caacctctaa
    20101 tgatttattt aatctatcta aaccagagct tatggaaatt gctaaaggca agaatataaa
    20161 gggatattca actaaaacta aagaggaatt aattcaacta ctagatgagt acaatgcttg
    20221 ataatgttaa attagtttta ggaatagaaa atgatagata tgacaaatta ataatccttt
    20281 atataaataa aataagtgag atggttttag aatattgttc taggcaggaa ctaagtacag
    20341 cactagaagg gtttgtagaa gaaaaagtaa tatcaatatt atcttcaaga tttgaagaac
    20401 ttaagaaaaa tagtggactt gataatctag aaaaaagctt aaaagctata actagaggtg
    20461 acactaagat agaatataat gctttaacat tacaaactgc tgaaaatagc atcagttcat
    20521 catctttttt aactgatact gataaaaaat ttctaagccc attttgtatt gttagtatgt
    20581 actaaggggt gattaaatga ctgaagcaga tatattagaa atgacatacc ttgataaaat
    20641 gactataatt agaaaattaa aaggctggaa tgaagaaaca tgttctaatg attttagtaa
    20701 tgttgttata gctgaaaata tcccatgtgc aatttctaga aaagaagaag ccattgtaaa
    20761 tggtgatatt ggatctatag ttgtaaatag aaagttattt tgtagaccag aagtagatat
    20821 aaaaataggt gatactataa atacaaccta taatcatgga gaagaagctg tatttaaggt
    20881 ttctaaggca aatatatatc caagccatat ggaaatacct attacagaat tggagaggat
    20941 ataatgggaa tagataattt aggagtcttt gaagcattag ccttagattt aggtctaaat
    21001 gcatcaaact ttaatagaaa aatcaagaag tttacaaaag aaactgggca agaactagca
    21061 gaaaatataa gagatgaaac tcctgtaaaa actgaaaagt taaaaaaggg atgggaagaa
    21121 aagcaaccag aatatgatac ttatattgtt aaaaatgaag ttgattatgc tactttagta
    21181 aataatggcc attcaactaa aaatggtggc attgtccctg gttcttttat ggttgaaaag
    21241 ggaattgaaa gaacaaaaga aaatatgaca agtgcaaaag caaatttatt tgattggtag
    21301 gtgattagtt gcttacaaac agagacatta taatagcagt taataaaaga atcgaaaatg
    21361 agtttagata tatagatatt tttattaatg aggataatat acaaggcttt gaggaggctt
    21421 gtttttttgt gcaactttta cctgtttcta ataatattgc aaataaagtt ttagatacta
    21481 aaaagatatt catagatatt gaatattacc aaaaggctag aaaaaataaa ttaaatctat
    21541 acgacataca aagtagatta gaaaaaatat ttaacagaaa cattagagtt aaagatagaa
    21601 gtttaatcat tgagaatata tctccatcaa tcgcaaaaga tcctgtagga tataaactaa
    21661 cttttctaat gactgtatct tattttgaag aagtctattt tgaaaatgaa acatttgaaa
    21721 ttatggaaag tgtagattta gagataggaa gtgattaaat gggtttacca gaaattaata
    21781 tttcttttat ccaagcagga aaaacttttg ttaaacgctc tggtggtatt gtagcgctta
    21841 ttttaaaaga tacaaaaaat atagggttag ttgagataaa agaaatagag gatattccag
    21901 aaaattattc tgctgctaat ttagattata taaaaatggc tatgaaggga aatgtatatc
    21961 ctccaaataa agtattagtt tatacattag atacagatga atcaatagat gatgcactag
    22021 attttcttga aacttgtgag tttaattatc tttgtatgcc agatgagaca gaacaagatt
    22081 tgcctaaaat caaaacgtgg attaagaaaa tgagagaaga taataaaata aaggttaaag
    22141 ctattactgc aagtaattca gcggattatg aaggtataat aaactttaca gcaactgata
    22201 ttgaagttga aggtaaaaaa tacacttcaa atgaatttct gccaagaata gcaggtttta
    22261 ttgcaggaac tccatcaact caatctgtaa catatgcaga aataccagaa gttactaata
    22321 tacctaagct aacaagagct gaagcaaata caaggataaa taatggagag ctaattttaa
    22381 taaaagaaag tggagctata gtaatcgcta gaggagttac atcctttaca acaattactg
    22441 attcaaaagg tgatatattc aaaaagatta agttggttga tacactagat caaattcata
    22501 acgatattaa aaaaataatt gtaaaaaatt atataggaaa aacccaaaac acttatgata
    22561 ataagtgttt acttattgtt gcaatacaat tatatcttca agaattagaa aaagatggtt
    22621 taattgatga aggttcaact gtagaaatta atttagatgc tcaaagagca tggctaaaaa
    22681 aacaaaatat agatatatct aatatggaag aacaacagat aaaagaatac aatactgaca
    22741 ctcttgtatt tttaaaagca agaattaaat taatagatgc aatggaagat gtatatttag
    22801 acatctctat gtagtttgga ggttaaaatt tgaaagctaa aatagatgca aaaagaataa
    22861 tatctggtac ttttggagag tgttggctag ataataagca acttacatca acaaaaggga
    22921 tggaggccaa ggttgatata gataaagaag aaataaaatt atgtggtcaa ataatgacca
    22981 catataaaat gattggtgca actggaaaag gatctataac tctttataag ataagtacct
    23041 tatttggcga gtatgtcgaa aaaatgttaa atgaaggtaa agaatttaaa gtaactataa
    23101 taagtaaatt gcaagaccca gattcatatg gttctgagag aattgcactt tatggatgta
    23161 tgtttgacga cttaactttg gctgattggg aagctgcgaa agttggagaa atagaagttc
    23221 cattcacatt tgaaggcttt gaatacttag ataaaataaa agaaaaatag gaggatttta
    23281 ttatatgaac acagtagaaa aattattaaa tatggatgca ggtaaattaa aaatgccatc
    23341 tagcgcattt gaattatatt gtaaaaaact agatgatact ttagaaatag agtgcaaggc
    23401 tattgatcca gaacgttttg acgaaataag aatgaactct atggacgtta atagtggaga
    23461 tgtagaaaac attaacatat atgaacttaa agtaaataca atattagaat catgttctat
    23521 atttagcaat atggaattag ttaaaaaatt tagtgcaatt acacctaaag aattaattaa
    23581 aaagctatta cttagtggtg aaatagataa attatacgag gaggttaata aagtaaatgg
    23641 aatagatgca gagggagata aaaaaagaca aaaagaaaga gctaaagata taaaaaacta
    23701 attaaaacgg atggagaagt taacttgatg tacttattat ttaagtataa aggtataatg
    23761 ccatccgttt cttatcatat actaagaggc gaaaaagaaa ttgttagtgt ttttatggat
    23821 caagaattag atgaaagaaa agaagaaata aaatctggtg ttgggaggtt gttttagtat
    23881 atgagttcta atacaaaggt cctacaagca ataataaaaa tgagagatga agcaagttca
    23941 acattaaaaa gagtagcagc aaatactaga gctctacatg attcatcttc ccaagcatca
    24001 agagcattaa ataatgttca aagtaattta aaaagtgttg gaactagtgc tttaaaagca
    24061 ggaggtctta tcactgctgg agttggtggt gcatttgcag ctgcaactaa agttggaaca
    24121 gaatttgaaa gcgctatgtc gcaagttaag gctacatttg gaaattcctt aaaaccagga
    24181 gattttgaag ctctgtctaa ggctgctaga gatgctggaa aagctacaac taaaactgct
    24241 gctgattctg ctaatgcact tcagtatatg gggcttgctg gatgggatgt tcaaaagtca
    24301 atgcatgcct taatgccagt gcttaattta tcagaagcag cctctatgga tcttggtcta
    24361 gcatcagatt tagtaactga tagcatgagt tccatgtgct tagaagttga taaggtcagt
    24421 aaaagtggaa aagtactagc tggtgcaggt ttaagtgagt atttagataa agttgcaaat
    24481 gcatctacta aatcaaatac aagtatacaa cagttgatgg aagcgtttaa tgtcgcaggt
    24541 ggtacattta gcaatttaaa agttccactt tcagaagcaa ctgctatcat gggaatactt
    24601 gcaaatagag gagttaaggg tacagatgca gggcatgctt taaactctat catggtaaat
    24661 cttacaacag gtgcaggtca agcaggtaag gcaatgaaaa agttaggtgt aagtgcattt
    24721 gataatcaag gtaaatttaa aggtatgtca aatgtgctta aagaggttgc aaataaaact
    24781 aaaggtatga ctgatgaaca gaaaaattat tacttatctg ctattggtgg aaagactcaa
    24841 ttaaaaacgt tacaaaagtt acttgatggt gtgggaaatg aatatgatag cttaaagaaa
    24901 aaaatagata attcaaaagg tgctttaaaa acaatgtctg atgtaatgaa ggataatact
    24961 aagggtggac ttgcaacctt aaaatcagcc cttgaagaaa ctggaattac tatttactat
    25021 caactaaagc cagcaatagc atctgtagtt gataatttaa ctaaaatgac tgtttggttt
    25081 aataatctaa gtgaaccaat aaaaagagtc ataactaatg taatgctttc agttggagca
    25141 tttggagtac ttttattaac tataggggct gtgtctttaa ttgtttcaaa agctataggt
    25201 atatttggct tttttgttcc aataatatta agagcaaaag ttgctttcag agcttttgta
    25261 attggtatga aaccattaag tgcagtattt ttagctgtaa tgggtcctgt gggatggact
    25321 ataacagctg taattgcatt atcagtagca tttaccattg cttatgctaa atctgaagta
    25381 tttagaaata aggttaattc attagtaaaa tcatttacag aatttgcaaa gcctatagta
    25441 aatttttgta tgcctgcact taaacagcta ggagagaaat ttaaagagtt gttatcagca
    25501 tttggtccac tatttgcatc tattttaaac ctaggaagtg taataatgaa ggtactagga
    25561 cctgttttaa tcggcgtaat aggttttcat atagcaaaat tagttttgat tttatctact
    25621 ctggcaactg cttttaaagc tgtagttgat actataacta atgtattaaa tggtctagca
    25681 tctattttaa atggagtatt tgatttaatt ataggtattg tcacaggcga tagtaaaaag
    25741 atgatggatg gagttaaaca aatatttgat ggtggaacta aaattataaa atcaatttgg
    25801 aaaggtttca tagattttgt aactgctcct atacaagcta tagctgatat agctgacagc
    25861 ttatttaaag ataaagctaa atggcttaca gataagtgga gagatttaag aacattctta
    25921 gaaaatccaa ttgaagcaac tgccaaaata tctactaaaa caaatgtaaa tatcccagga
    25981 agtaaagcag cagatgcagc tggtaaatct acaccaaata caggtggatt aaaacaaaca
    26041 gtagcaaatc taaaaggttc atttagtgat tttaaaactt ttattaagcc tatgacagac
    26101 tctataagtc aaggagttga atctggaaaa actaaattta cagaactgca aaataaaata
    26161 ttaaatgatg ttaaacctgc actaaaggtc ctagaggatt ctggagtaaa ttttaaggat
    26221 aagtttaagg agtcaataac ttcaataaaa gataaattta atgaattaaa gaacatatta
    26281 gctggaaata ttaaaacaaa tatagataat ttagtaaaag catttgaacc tttaaaacct
    26341 catttagata atttgaaaga ttgctttgat aaggttaaaa aagcgattac tgattttttt
    26401 actccagcca aacaggttgg agatacagta aacaaagtaa gccaaccaat ggatagtgtt
    26461 aaaaagtcta ctgatggaat taaactttct ttccaaagtt taactcaagc acttgctcct
    26521 gttaaagctt ggtttcaagg atttatgcct ttcctatcac aaataggaca acaactttta
    26581 ccatctatag gaattgcaat agctggtgtt gttggcggaa taattttagc atttacatgg
    26641 gttttaaata cattgaccgc aataataaca acagtatctg gaatcataaa tggaattatg
    26701 tcttttatat ctggaattat taatgttatt atggggctta taacaggaaa tgctgatcaa
    26761 actatggatg gacttaaaca aatatttgat agtggagtag gtgtaataaa atcaatttgg
    26821 aatggccttg ttgatctagt tagtggcgta ctagaacctg taatagatct tgctgattct
    26881 cttttcaaag ataaagttaa aggggtaaaa gaagcttgga ataaacttaa agatttcttt
    26941 aaacatccaa ttaagggaac aatagaaata tttaaaaaag gtcctagttt acaaattgga
    27001 aaaggtgcag atgctgctgg caaaagtcca agaaaagcat ttggtataaa tagagttcct
    27061 agaaatgatt atttaataag agcacacgaa ggcgagaaac ttttaaccaa acaagaagct
    27121 aatcaataca aaaggaatag ttcaaatgga agtttatttc ttgataaatt ggctgataca
    27181 ataattgtaa gagaagaggc tgacatagat aaaattgtaa gaaaaataaa caaaaaaata
    27241 tctcttgcaa aaattggagg tgttttgtaa attatcgtgc tataattgta gtatataatt
    27301 tacagggggt tatgttatga ataaaaaaat actagtatta acaatatcaa ttatactatc
    27361 tttaagttta gttggatgca gcacaaataa ttcaaaaaat agagatagta aatctaagca
    27421 aaacacagag gaagttaaaa atcataaaat aggcgaagtt gttgatttag atggagcaaa
    27481 agtatctgtt aaaaatatta ggaaattaga aacaaatagt aatgattatg ctgttgaaat
    27541 tgaaataact aatacaggtg ataatgattt acaaagtgat tatctagaaa actatatatt
    27601 aaaatcaagt gacaatacta ctggagaagc aataaataac caagaattaa atggagagtt
    27661 aatgtttgat acaatacgcc caggagatac tttgaaaggt gaaatagcat tcgaacttac
    27721 aaaagatgca acaccaaaaa cacttgaaat tgcttatgaa aatgtaggca aatatacagt
    27781 atttgattta tagtaattat cttaaaagac gcttatttac ttaagtgtct tttattttag
    27841 gaggagttta atggaaatat ggttaagaca agcaaataat acatttaggt ttcctgttat
    27901 tccaccctct tttgagataa atggaagtgc gacaataaat acatctaata ttttaagtgt
    27961 tggagatatc gcagtatttg gaggattagg gcttaaaact atagaactat cctctttttt
    28021 ccctaatcaa gaatatagtt tctgtaatta taatggattt ccaaaaccat atgattgtgt
    28081 aaatttaata gaaagttgga tgaaagaagg atatatatta agatttatca ttacggaaac
    28141 taatataaac tttgaatgta taattgcaga ttttaattat agagaacaag attattcaag
    28201 agatatttat tttaccttaa gtctaaaaga atacagaagg atacaaatat ccaaagtaaa
    28261 cattaataat gatgaaaaat tatcttctga aaagaatgtt cctctaacaa aaggatttga
    28321 tactaagcaa aaaacgcata aggttgttga aggagatact ctttttaaga tagctaaaaa
    28381 atattatagc aatggagatt tatgggagaa gatttataaa gcaaatgaag atgagattaa
    28441 agatccatct gtaataaaaa atggttgggt attaataatt ccttaaaaat ggtggtgatt
    28501 tataattaat aaaatcaaaa tacaagttca tataaaaaac ggaaatatat ataatgtaac
    28561 tgatttagtg gataaatgca cttggtctgg tgattataga tcaccatcaa gaactttgga
    28621 attttctata attcaatctg caagtgatat taattttagg cagatagata ttccagtagc
    28681 aagtacagtt tgtttctatg tagatgataa agaacttttt agaggaatga taatagatag
    28741 atctaaagac tctagcaata ataatataga ttttacagct aaagatatgg gttttttact
    28801 tttacaaagc gaagtgtcat acaattttaa agataaatta gtcgaagata ttgcaaaaca
    28861 ggtttttgta gataataaat tcccactagg taatttacct aaaactaacg ttaaatatac
    28921 aaaaatgttt attggtgtaa ctggctacga tactataatg agcgtttata cagaagcaag
    28981 caagacaact aaaaaaaagt atatgataga gtctaatctt gataaattta atgttattga
    29041 gaagggtgta gttactttaa atataacatt tgaagaaggc tttaatctta tcaatacaaa
    29101 tttttctgaa agcattgaga atgtaaaaaa taaagtacta gttgttgatc agtatggtaa
    29161 taaaattagt gagaaaataa atgactctat atttaaagac gttggggtaa ttatgcaaaa
    29221 ggtaatacaa caacaagaaa acagtactat agacatagat ggagagttta aaggaatcga
    29281 aaaaacttgc tctttaaaag gatatggtga tataacttgt gtaactggta gaggtgtaaa
    29341 agtaaaagac tcatatacaa aattaatagg tcttttttat atagatacag ataagcatac
    29401 atggcaaaat ggagattatc aaattgaact agagcttaat tttgagaata taatggatga
    29461 aaaatctgct ggccaagatg agcaaaaaga agatggttca gagtatactg gaggaacaga
    29521 ataccctgct gaatttacgg cttattgtcc tagaaaagaa gagggtggaa atacagattg
    29581 tagaggtaaa aaactagatc cttctaaaaa gacatgtgcc gctcctatgg ttggagcata
    29641 tgaaaaatct tactatacaa aagatttctt aaacaaacat cctcttatga gatatggaga
    29701 tgaaatacaa ttagttacag gtgtttcaag ccgagatgga acgtataaag taaatgataa
    29761 tgggcctgca ataattatag aaaaggatgg aacatatcat atagatattt tatttggaaa
    29821 tgttgaagaa gctaataact ttggaagaag aaaaggaaag attataattg gtggttattc
    29881 tggaaacatg acagaaaaag caaaaacagt aatttcagaa gcaagaaaac atcttggaaa
    29941 gccatacaaa tggggtggga atggaccaag tagctttgat tgttctggac taatggtata
    30001 ttgctttaag aaggttaatg ttaatttacc aagaacctca agtcagcaat ctaaggtagg
    30061 taagaaagta gagaaaaata atttacaggc aggagatttg gtatttttcc ataatccagt
    30121 cagccatgtt ggactatatg taggtaatgg agaattttta catgcgccac aaacaggaga
    30181 tgtagttaaa ataagtaaat taagtaatag aaaagatttt aatacagcta gaagagtttt
    30241 gtaaaagagg tgatatcgtg gcagatccaa tcaatgaatt tataggaata attaggcaag
    30301 aaggtaaatt tcataatgaa ccttcttttt ttattggaaa aattaaaagt aaattaccag
    30361 atttaaaaat agagataaat aacatcatat tagaaaaaga agatattttg atagatagtt
    30421 ggatgcttga tagacagata gagttatttg atacagaaac aagtcaagaa cataaacatg
    30481 aaataaaaaa tccctttata gatacttttg aatctggaaa cacagtaata atgtttaaaa
    30541 taggtgaaaa atttgctgtt gtaagtaaat tggtgagtct agatgaataa tactatattc
    30601 cctttcatgg gtgttccaga agattatatt tctccagaca atgaagaatt acctattttt
    30661 aaagaatttg cttgggattt taataaagat gaaaagataa ttgaaaatgg agactttaaa
    30721 atagttgaaa gaaatgaagc aattaaagta tggatttaca aaacaataaa aacagataag
    30781 tatgttcatt taatatatga ttggagttat ggaacagata taaaaaatct aatagggcaa
    30841 aagtatacta aaggtcttac tgaaagtgag gcaaaaaggt atatacaaga agctctatta
    30901 actaatccat atatattaga agtcaatata acaaatgcag aatttgaaga tgataattta
    30961 tctataagtt taaatgtaaa aacaatttat ggagaggagg agattacttt tgtataatgg
    31021 ccaatcattc agtaccttaa ataatagaac tttaaataac ataaatctcc ctctttataa
    31081 aggccaagga tcttctcttt ataatatagt ttctccaata aattcagaac ttgcacaatt
    31141 atatatagaa ctttcttata tacataagag agtttttatt caagataatt ttgacgattt
    31201 tcttgataga agagtcaatg aatttggcgt atatagaaaa ttaggtacag aggcaatagg
    31261 tgaggtaaca tttgagggca aaattggaac tcaaatacca aatggaacaa taatatctca
    31321 tagtgattta ctatttgtaa ttattaaaga tattacaata gatgaaaata gtaaattaaa
    31381 tgtaagccct atacaggctt tagagattgg cattaagtat aatttatcag ctaacacaga
    31441 atttaaactt atagaagaaa taactggagt aactaaaatt tataatgaac ttgacctaaa
    31501 gggtggaaca gaaatagaaa ctgatgaaga attaaaagaa aggttctata aaatacaaaa
    31561 aaatcaagcg acaagtggaa ataaggcgca ttaccaatct tgggctttag aggttgaagg
    31621 tgtttataat gcaaaagtaa ttcctcgttg ggatggtcca ggtacaatta aaattctaat
    31681 ttatggtcaa aacaatcaat cagttgacaa tgaagtattg caaagatgta ctgaacatat
    31741 agaagaagaa aaacctattg gacctactgt tacaattgtt acaccaagta cttttgatat
    31801 taatattagt gcaactttaa cacttgaaaa tggatatgat attgaatcta taaaagttgt
    31861 gtttttagat attataaatt cttatttgat agaaaattca agagagattg tttatataaa
    31921 agtaatgagt ttgttagcaa gcatagaagg tattcatgat ataaaaaatc tccttgtaaa
    31981 tggtgatgtt aaaaacataa tagttgatga agaaaaagtt cctgctgttt caagtgctat
    32041 atttgatatc gaggtgagtt aaattgaagt taattgatta cctgccaagt tttgcaaaca
    32101 atgaaattga catacaaatc caagaagcac ttgaaaatga acttttaacg ctaattgatg
    32161 aaaaagatga tttattagaa cagtttttta tagatacagc aacttgggga cttgatgatt
    32221 gggagaacct tttaagcata aaagtaaatt ataagttaga cttcgatact agaagaagca
    32281 atataaaagc aaagatgaga ggtaagggta caacaacaat agaggttata aaagctatct
    32341 cggaagctta cacaaagacc aatgttgatg tagaagtatt tagtaatcta tttagtttta
    32401 cacttagttt tataacaaat aattgtagtt ataacactat tttagaatta gataagaaaa
    32461 tagaagaaat aaaacctgca caccttgaac acaaattcga gaggatatta tttaataaaa
    32521 acgagcttta tacaggtgca gcaattagta caggagaaac agttacaata tatccttatg
    32581 tacctagaaa tttagaaagt tttggagaaa tagctatttg tagtggaaat gatagagcat
    32641 tagaaaaagt aacattgtat cctaaaaaat agaaatgagg tgataaaatt gacagaacaa
    32701 caatatttta ctctagtaac tgacattggt aaggcagcaa tagcaaatgc aagtattaca
    32761 ggtgaaaaag tagattttgc aaagataaaa gttggagatg gaggagggag ttcttatact
    32821 ccaaatgaga gtcagacagc actaaaaaat gtggtttggg aaagtacact tgaacatgca
    32881 caaggagata aagataatcc taactgggta gtaatacaaa aattcatacc tggtgatgtt
    32941 ggaggatttg aaataagaga agttggtcta tttgattcta aagaccaatt attagcgatt
    33001 tctagttacc caacaacata taaacctact gcggattcag ggactgtaaa agaactatta
    33061 ataaaagtaa tattagttgt atctaatgta gctaatatta atttgaaagt agacccaacc
    33121 gtaattttag caactttaaa agatatacaa gaattagaca ctaaaataga tacaactaaa
    33181 acagaattaa caagcaacat agaaactgct aaaacagagt tgaacactaa aataggagat
    33241 acaacacaac ttactacaac agataaaaca aatatcgtta gtgcattaaa tgaggtaaaa
    33301 actagtgtag atagtataga aacaacagca gataaaacaa gtataaaaga tacagataat
    33361 ttatttgaga gcgacaacgt agagggagca ttaaaagaag tgatgcaaga agtaaaaggt
    33421 aatagaagca gtattatatc aacaataaat agtaatttaa taccaatgta gaaagtgagg
    33481 tgataaagtg aagatatgga aatacaatag aactgtgaag aaaagaagag gtgtctatac
    33541 agaattagat atacttgcac aaaggtatga atttgaagca gatgggaaac tttatacagc
    33601 ttattttcat ttatatacta ataagaatat agatatattc agagataaag atttatataa
    33661 agaatcttat atgatgagct atagagttgg aaatgtaaca actgatattg atgatgtagt
    33721 acagcaaaca actacagtaa gatttatacc tgaccctgtt attgatattg tgattgataa
    33781 cattagcaga ggatgtgaaa taaactacag aattatagat aatgaaccaa gtgtaaaatt
    33841 cattgttaca gaaaagttaa atggcacaat aatagaaaca aagacaaatt ctagtgataa
    33901 taattataaa cttaaattaa ctgatgaaca aatagtacca ttagcattca attcccaaaa
    33961 ttcaatagta atagaaataa gtacagaaga tggtggatta gtaacaagta aaacagtaac
    34021 atttacaaga acaaataaca agccaacaat tttagttaat tcatatagtt ctaattctgc
    34081 aaaatttaca gcatctgatt tagataataa tttaagtaaa attgagtggt atttagatga
    34141 tgtattaaaa gaaacaataa caacagattt aacagcagaa aaaaccatca actatgaatt
    34201 tacagacaat gcaatacaca cattaaaaat agttgctaca gatgcagaaa atgcaacagt
    34261 tgagaaggtt ttaagtataa gtaaagagat aatgccactt caatctgatg ctagtttaag
    34321 tgatatatca actaagctga tagagattgg agaagggttt agaaatggta aaacaagtat
    34381 tataaacact ttagcactta aaaatataga agcaagttta aataatacac ttgtggagtt
    34441 atcagagaaa ataaaaacaa gttttgatag ttcagacgct agtgtgcagg atttgcaaaa
    34501 tagaataaca gaattgaata atcagcttag tcgaagaaaa aaatgggcta caggtagata
    34561 cacatttact agagaagaag atactatcaa tttcagaatg gttgatggag atagtctttc
    34621 cgcagtaaaa aaactaatta tacccattaa tttagatttt gttccaagtg ttattataat
    34681 agacaaaatg tttttaggta gttacgtacc tgcacaagat gtgcaattta attgtgtttc
    34741 aaacttagcc gattttacac ttggacgtcg tttctataat aaacaatgga tttgcacttc
    34801 ttctataaaa ctagattcag ttacacaaaa tagttttact ctttctttac aaaaagttgt
    34861 tccatttgat aaagttagta gtacgatttt ttctattatt aatactggtt ttgcttggag
    34921 agcatatgaa taaaaatata aattagaggt gattaaatga atagaaataa tagaataatt
    34981 tacgaccaaa caggcaagat atggcttcaa actggtgaag caataggaga tatacaagag
    35041 ttgtcagaaa taactgaatt aaatttcata gatgttgaat ttggtagtat agactatagt
    35101 aaacaatata tagagtctat aaatccagtt acaaaagaat taaatataaa aaatatagaa
    35161 gttgttttga ctgatgaaca aaagagatta gaagtattag aaaaagaact aaatatgtta
    35221 aaagaagaaa ataaaaatag agattctgag atagtaaata cagcatttga agtaggaaat
    35281 atcaaattaa acaataattt ataggaggaa attatatgta taacttatta aagttaatga
    35341 tagaacaaaa aaactatagc actaaggagg atttacaaca taagatggat gtattctatg
    35401 cagtaaatag gattacagaa gaacaatatt tagagttaac aggtttatta aataaagaag
    35461 aaacaccagt agaaccaaca atataggttc ttttttattg aaagaaggtg actaaatgac
    35521 ttttaaagaa ttagttagta aagttagaaa tcttgtatta gaagcaaaga atgtaactat
    35581 agaagataca gagaataaat tcactagtga aaatgtcgag ggagcattga aagagtgtat
    35641 agatagagca gatttggctt ttcaaagtgc cgatagtgga aaagagttaa ttgcaactgc
    35701 tatcggctct cctgctacat ccgaacaaac atttcaagag tatgctaatt atattgcagg
    35761 atttaaaaat actataagtc agttaaattc taaatataaa tatactagtg gaacttttac
    35821 ctattatact ggtgcatatc ctaatgggct aaatataggt tttagaccag atattatttc
    35881 ctatactgcc attaatgctg tagaggaaaa atattattgt gtatacacac ctttattcta
    35941 tcttttttat aattatacaa ctggagaaaa atatcttgat gttaatcata atcgttatgg
    36001 aataactttt actgacaatg gatggatacc tagaaatcca gttaacaatg gtgtgtatag
    36061 ttatatagca gctaagtgcc gttagttgtc aaagacttag ataaatttct aagtcttatt
    36121 ttaatacaaa attaggagga agtatgaacg taacaatagt ttttttagca acaaatatat
    36181 ttataaaatt agtaatatta gcaatagcat ttgatacatt gctaggttgt ctaagagcaa
    36241 taaaaacaca tcagtttaat agctcttttg gaataaatgg aggaataaga aaagttgcaa
    36301 tgatagcatg tatatttttt ctagcagtag ttgacatact tacaaagttt aactttttat
    36361 ttatgttgcc acaagattgg gttgatttct tacgactaaa tcatctagga atatctgaat
    36421 ttttctctat tttattcatt ctatatgaaa gtgtaagtat attaaaaaat atgtatttat
    36481 gtggattacc agttcctaaa cgattaaaag agaaaatagg tagtttatta gatactatga
    36541 cagatgaatt aaatgttaaa ggaggaaaat aaataatgaa aatatgtatt acagtaggac
    36601 acagtatttt aaaaagtgga gcatgtactt ctgctgatgg agtagttaac gagtatcaat
    36661 acaataaatc tcttgcacca gtattagcag atatatttag aaaagaaggg cataaggtag
    36721 atgtaataat atgcccagaa aagcagttta aaactaagag tgaggaaaag tcttataaaa
    36781 tacctagagt taatgctgga ggatatgact tactcataga actacattta aatgcaagtg
    36841 ataggcaagg aaaaggctca gaggttctat attacagtaa taaaggtcta gagtatgcaa
    36901 ctagaatatg taagaaacta ggtacagtat ttaaaaatag aggagctaaa ttagataaaa
    36961 gtttatatat tttaaacagt tctaaaccca ctgcaatact aattgaaagt ttcttttgtg
    37021 ataacaagga ggattatgag aaggctaaga aacttggata tgaaggtatg gctaagttaa
    37081 ttgtagaagg tgtattaaat aagagtattg aaagtaatga ggtgaagcaa atgtataaac
    37141 acacaatagt gtacagtggt gatgatagag tatcagcaga tattttagga ttatattata
    37201 agagagcaaa agaaagttat ctagttatag atataaaaga ctataaacca catagaacac
    37261 aaaatctata tgtaattggt ggagtaactt gtaataaaat gaaagaaatg agtaagacta
    37321 caggagaaaa atttactcaa ctatatggta atgatgtatg gtcaacaatg gataaagtta
    37381 tagaatttgt aaaagaaaaa ttataaatta aaagacaacc tttaaaaatt aattgttaat
    37441 aaaactaata aaattttcaa ctcagaagga gcattatatg ttaacatttt tagatttgtt
    37501 tgctggcatt ggaggtttta ggctagcaat ggaaagagca ggacacaaat gcgtaggcca
    37561 ttgcgaatac gataaatttg caaacttaag ttacaaagca atgcataacc caaaggagga
    37621 tgaatggttt gaaaaagaca ttagagaaat tagagcagaa aatattccca aagcagatgt
    37681 gtggtgtttt ggattcccat gtcaagacat tagcattgca gggaacaaac ttggattcaa
    37741 aggaaatcgt tcaagtttat ttttctcagt tacaaaactt attagagaac ttaaagaaga
    37801 agatagaccc aagtacttac ttattgagaa cgttaaaaat ctacttagtg ttaatagagg
    37861 atttgacttc gccaaacttc tcattgagtt ggatgaaatc ggctacaatg cagaatggca
    37921 aaatctcaat tctaaaaact tcagagttcc ccaaaacaga gacagaatat acattgttgg
    37981 acatcttaga agaagaagta caagagaagt atttcctatc acaggaacga gtagaaaaac
    38041 tactcttgag caattaaata atcctaatca cagttcaaac aggatttata attcaaatgg
    38101 attatcaaag tgtttattaa gtaaaggtgg gaacaataca ggtctttata gagttggcaa
    38161 tatcaatcct agtggaaatg gtgcaggcgg acaagtgtat agtagcgaag gcttttctcc
    38221 cacacttaca actggaaaag gtgaaagccc aaagatatta attaaagaga atactaaaaa
    38281 aggatacatc aaagcatcta taggagatgg gataagacta gatcatatag aaggtaaaac
    38341 tggccgtgga agagttcagc acaaaaaaag ttgtactttg actactgcaa gtaatgctgg
    38401 cgtaattggt aaaaaattta gagtaaggag gttgactcca aaagaatgtt ttaggttaca
    38461 aggatttcca gacgattatt acgatagagc ggcaagtgtc tgttcagaaa ctcaactata
    38521 caagcaagca ggcaatagtg ttactgtaga tgttgttgaa gaaatagcaa agaaattaaa
    38581 atagaatatt aatacaatgc aaaatgtggt aggtttttcc ttccgcattt tgcattgcat
    38641 tagctttttt ggaataactt acctgtacta tcttaaataa aaaaatgcta ctatattata
    38701 aacatttaca aggagtgcat aatgttaata caaagcttaa aacgtggcga attaattgta
    38761 atgaaaatct tttagaaaag aaattcaaga atatcaaaga aagaaattat tcgcatttgc
    38821 aatgaaaaac acaattggcg gaaatctgcg acaaaaactc tacttaaaag acttgttaaa
    38881 aaaagagttc tacttaagag aattaaaatt ttccacatat attacatacc tatagttaca
    38941 gaaaaagaat atttcaaata taaaataaat gatttagagc cagattattt agatgaatat
    39001 tttatacgat taatgtccac tgtacataaa tttaaaaatc tagatgaaga agattaaata
    39061 tctcccttca tctactcttc acatatttta atagactttc aagcaataat tgacttaaca
    39121 aatcatgctt atcataatgt ttattctcat ctgcaaattt attaaacatt tcccacactg
    39181 tattatttat tctaactgta gttcttttta tagattgatc tggaagatct atagatatat
    39241 cattttcttt aatgacacat atatgataac tatcatcttt agttttaaac cattccagca
    39301 cattctttat attatcaatt tcactagata aatatttaat atcgtcatca atcttactgc
    39361 taatgctatc tatatgatat ttatcatcat aacttttatt atcttctaca aacttgtttc
    39421 cagttctttt atatcctttc ttattaagcc acttccttat agtactttca tttgccccta
    39481 attcatctcc aactttttta agactttcaa attcacttaa tttattatta atacaatcaa
    39541 caacttcatt aatatttttt tctttcaaat cactccaagt catataaatc ctcctcaaaa
    39601 aaataataga ttacaaatat catatatttg taatctatta tacatactat aaactaaatc
    39661 cttttttatt tcatcatttt tttgtatcct tttgcatttg caaaaacatg tttatctaca
    39721 tcactcataa ctattatgcc aggtatattc tttttaaatg cttcacctaa caatttagcg
    39781 cctccaccca taacaacaac cgcagctgta ctaactggat aatttaattt caattcattg
    39841 tatatttctt taaatagaga aatagatgca tctatatttt tctttatatc ttgtttttca
    39901 ccatttaggt aaaatccttt atttatgtag tcttgtattt tttctctttt tatctcaact
    39961 ttacctaaaa atctttcgtc catctcaaga gatttcttta ttgcatcata tatatttatt
    40021 gttcccacat ttatagaata tggatcagtc gctttattcc cttgtacaag cgcaatatct
    40081 gttgtctttc caccaatatc aactaaaaca acatctcttc ctgcaatttg gtcaactatg
    40141 tcttttgcat aatataagta tcctgtaaca ccttctggaa atgtttgaat agatcttatt
    40201 gttatatctc ttttagtagt ctcatttcct cttgtaaact caacatgata ttcttttcct
    40261 tgtaattttt caataagatt ttttcttaat ccggcaaatt gtttcactgg cagacctagc
    40321 cctagatcaa caatttcatc atctgtattt ctacatatag cccctaatag tagtggtaag
    40381 aagttttctt tctcatattt aactatattt atttcctcgt caccttcacc taggtaatat
    40441 gttttattgc ctattttaat gctatctgaa tctgaaccaa agttttttac ctctgtaact
    40501 ttactttcaa aaatatcatc tgtacttgtt ttaactgcat aatttccaat atctatacct
    40561 aatttactca tttatgttat ttccccctta ttattctaaa cttccaaaat caaaattgtt
    40621 agctggatct tctatttctt ttttctgttc aactacagca ttatcctttt tttcttgcat
    40681 tttttcccaa acaagctctt ttaagtaagc tgtagctgat aatttctcat ctaaaaactt
    40741 ttcaattgct ttttctttat cattgttttt aaatgttaga actatcctac tcattcaacc
    40801 acttcctttt ttataaaata ttataaaatt tattaatatt tatatatatt ttactaaatt
    40861 ttacacagta attcaataga tttataagat attttttata atattttata attttttacg
    40921 taattttata attttttata aaatttatca ttttgtatat aattaaaaac acacttttta
    40981 taattttgca taaaaaaaga gtgtttcata tatttctaaa cactcttttc tactttttat
    41041 aattatctaa ttgtaaaaga agcttcagct ggactagaga ataatataat acctcctgta
    41101 cacacacctt ttcctttaaa tttagcaata tatgaaccct tttttagccc tttaaaagtt
    41161 actacaccag ttttagtagt tccagctaat ctaaagccta catactgaga tgatacaatt
    41221 ttgccagcct tatatacatc tatatttcct tcaaatccta aaatatttcc aagcgtagat
    41281 gttactctcc aagataactg cccaccactc attgcagtta aattagcagt accacctgga
    41341 acaacaacag atttagtcga aggcgaatct gagtttggtt ggccaattaa ttcaactgtt
    41401 tgtgaatttc cagtaccttc ataatcattt ccatcagcaa aagctgtaaa tggaatcatt
    41461 actgcgcaaa gcgttagtaa taaagcaatt gtttttttca tttgttttcc ccctattttt
    41521 ttatattatt ctcaattaca gtttcctttc tttacgtgcc ttaataatca tattgtaatt
    41581 atgctcagca gtttcaaatg ctcctaccaa aggagttata attacacttt ctattaagca
    41641 caattcatca tagtttaaac tagcttctcc agcatagaaa ttatttaact tatctaaatc
    41701 tattctagtt aaaacggaaa ctgattccat tgagaatttg taaacatcaa taagtttata
    41761 taatccacct atatagtttt ttgctacttc atggctctta aaaaattcat cttttcctgt
    41821 atgtaggttt acttccataa ttccctccat tgttcttata tttttaacct tcaaataata
    41881 tttacttctt cttcaaaaca gaatattcta tcacggtaga atattctgtt tttttcattc
    41941 tatcatgata gaataatatt ttcaatcatt ttttctattc tattttattt aaatagaata
    42001 gaaaaaaaga acacttaata tttttcaagc taatatatta agtgttcttt ttatattttt
    42061 acaagattat ataaaattat tctttaatca attaaagtgt ttttctattt cttaataata
    42121 agaaatgata taaaattgta tcattttaaa gattttgtga tataataaaa gcaagaagaa
    42181 ctacaatcta tttaggcgta gagtggagtt cataataaac gtagtttatt ttttgaattt
    42241 aaatataaat ttaaattcaa cctgtaagtc actctttgca cgagagtggc tttttgcttt
    42301 tttgaatagt tcactgatta agtaaactac cacactagct gttaaactag ctaaaacatt
    42361 aaacaaaaag ttatccatgt aactcacctc ccctcgaatc gttgggagga taatcttttg
    42421 tacatgaact ccactctata gattgtatat tacgctcttc ttgctaaatt taattatacc
    42481 atattttgta atatcagtta agagtttgca ctacaataat cagaaagctt gttccactca
    42541 caatcaaccc ctattatttt aagaatatca ttgacttggc tatttaaatc tttaatatct
    42601 gatttagaca ttaaaggatc tttcattatt tcatttagaa aaatactatc aatgattaaa
    42661 catacatttt ctgataaata ttcacgtttt actgagatta ctgttccaac tgtactttct
    42721 tttagtgtct tatatgctac actcaatatg catggttcta aattaattcc ttctagtact
    42781 gtttcaaagt aatattttat tcttatattt tcatttccaa taaatatttc ttcattgctg
    42841 cttatcttct ttaattttct cattatgcct aacatcttag cattcacttc atagtttatt
    42901 ccacatctct ttgatgctaa actcgacatc aagtctcttt ctgaataaat tacccattga
    42961 ccattatagg aaaatgatgt tttagcgata tccatttctc cttttccagt aatattaaat
    43021 ttaggctcta gtttatttat taagtaaatc tcataaaaat ccatagtata tttattatct
    43081 aattctatat actcaaatct taaattctta ttacaccaat tttctttttt attacttaaa
    43141 tgactagcgt gtctatcgca gatattctct gttttcccaa cgtataaaca ttcttccgta
    43201 tctagattaa tatatttata aacataatac attttttatt ttctccaatt attatttata
    43261 gtaatatcct tcttcattta ctagaccttg atcaaataaa tttctcctaa cagtttcaag
    43321 tctgacagga tctttgctat aatcaacttc tctttcttct tttttaacat cttcatcatc
    43381 ccaatactta tccaattctt ctttaaactt taaatcatct ttttgatgaa ttataacttc
    43441 acttatttta tttgaaagtg ttgatttaaa aaaatcataa ttctttgtat ttataatatc
    43501 agtattatct ctttcaaatg ctatagcaat agacttatta aaagcattca gataaacatc
    43561 tttcttaaaa tcaaactctt taaaatcttc tttaaatagt ttaatcgtcc ctgttgtaaa
    43621 aatattttca tttggtatat aaaaactaaa agacacttca ttgtcctgct tctttttagg
    43681 tatataatct tcaaccaata tatctttatc aaaatatttt ctaggttcat aatctatcac
    43741 agaaaaaatt atttcatcta tttttctatt ttttcttttt tcttctttta ttgaaacaac
    43801 catgttgcct tttttattta tttcattcat tgctcttttt agaacatttt gtttaaaata
    43861 tttatactct ggatatacat tatcttttaa tttcaaataa aatcttaatt catctaactt
    43921 atattttatc tccacttcct tattttctct gctccatagt ctaaataaag tgtataacct
    43981 ctgagtatat gcacctctaa aattaaataa cactgataaa ttaatagctg tatatccatt
    44041 tttctttttc tgcattttta caaaatcagt tatatgattg tatagaactt catgcatcat
    44101 aattgtataa atttggtctg tatgatccaa ttcatatgta ttaattaaac cacttccaaa
    44161 agtttttaac ttgccagttg tctcttctat atagtcaaat tccaaaacac tttgttgaaa
    44221 catgttcaaa atttctttta tatttttatg ctcataatca ttattgtttt tcataaatac
    44281 ttttagttct tcttttgata tagttgtaac ataagatgaa ttttgctgtt tctgagcatt
    44341 aaaaagtatt ttgtaaaata atttattttc cacatttgta aaatcatatt tactttttat
    44401 taaattattt ggttgcatta aaatttcttt tttttccaaa atctatcacc tcatataaat
    44461 tataatacct aatttataaa aaggtcaacc atttttgaaa gacgctcacc tttgaccctt
    44521 aaaatgatga cctttgaccc ttaaaatgat gacctttgac ccttaaaatg atgacctttc
    44581 gacccttaaa atgatgacct ttgaccctta aaatgatgac ctttgaccct taaaatgatg
    44641 acctttcatc tctgaaacct agatatacca attattatag cttgtctaaa aactattaaa
    44701 gactttaaaa actgttttaa aaactattaa agactagaaa agatgttctg aactattctt
    44761 tttttatatt aaaaaacttt agtatttatt tttctagtat actttaaaca cttactaaaa
    44821 ataatttctt ataaaagatt ggagatgaag aattttttat gcttgaaaac agaaaacaaa
    44881 ttcagaaaga aatttactta ccaatagcaa catattgttt aaaagataag caatattata
    44941 taaaagaata tggagcacta ttaataaaga gtgctgaaaa tagtgacatt tttaataccc
    45001 ataaaaatag aattttgtat aagaataaac ttgatattca agaaatagca aatgaactga
    45061 atgtaaataa agcaacaatc caaagaaata taaaaaaact agagaagtta gattttaaaa
    45121 ttctaaaaat agaaaatacc ctaaatggaa ttgtatattg tttaccatca gaaaataata
    45181 ttgatttaaa caaatttgca ttgataaatt atgaaatgct taaaaaaatg gtcaataaat
    45241 ttaaatcaaa tacaataaaa gtatattttt tacttaaaac aatcactaca gaaactaatt
    45301 ttaaacctgc aactaatagt tttatagcag aaaatatagg actaagttct aaaagtaaaa
    45361 ataatttaga tatcataact tcttctgtta aaactttaga agaaaataaa tacattgaaa
    45421 caaaaaaagt aaatgtatat gaatatgaca aagaaaaact tagagaagtt ccaaaaataa
    45481 gaaaagttta tcggatttgt aattttgatg agtggaaaaa ggaaattgat aaaaataaag
    45541 tttattaaat atagcttaaa agcatttaaa ggcttgtctg acgtgtttta attacaaact
    45601 tgatgttatt tacctaaaaa gatacaaatt tgcttaaaaa tggaaataga aggtcaaata
    45661 tataaataga gaaccaaata aaattatatt ggctctctat ttatcttctt cccaatcctc
    45721 aataaacttt tctaaacttt ttaatttatt ttcagacact tcttcatcat catgcagagc
    45781 acttataaaa ctttcaaaag aatttttgtg aaagaaacta aagaaacttt tagtctcaac
    45841 ttttaaatat tcttcttttt ctattaatgc tttataatat gtaaatctat ctttcttaac
    45901 aacctctaaa aatcctctac caactaatct cctcaagaaa gttaacatag ttgatttttt
    45961 ccattcgtac ttttcgccta gtgtcttcaa aatttcagta gatgcaactt ctttatcttc
    46021 gctgtcccaa ataaacatca taaccaacaa ttcactttct ggaatctttt ttatcttcat
    46081 aaagtacacc accttctaaa agtatatcta aaatactata acaatagagt taactacaca
    46141 atactaaatg tctaaaaatg ttttaccaat ttttaccaat taatttttct tt
//
LOCUS       DAYAOX010000005        45454 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.005, whole genome shotgun sequence.
ACCESSION   DAYAOX010000005 DAYAOX010000000
VERSION     DAYAOX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.005"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(82..2010)
                     /locus_tag="ACGI57_000231"
     CDS             complement(82..2010)
                     /locus_tag="ACGI57_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9715322.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            2223..2696
                     /locus_tag="ACGI57_000232"
     CDS             2223..2696
                     /locus_tag="ACGI57_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9715323.1"
                     /translation="MNGVEKYMVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNK
                     EALLKDLLKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRY
                     VFSLLAPHKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            2699..3007
                     /locus_tag="ACGI57_000233"
     CDS             2699..3007
                     /locus_tag="ACGI57_000233"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFG9715324.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            3121..4227
                     /locus_tag="ACGI57_000234"
     CDS             3121..4227
                     /locus_tag="ACGI57_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFG9715325.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            4302..6980
                     /gene="mngB"
                     /locus_tag="ACGI57_000235"
     CDS             4302..6980
                     /gene="mngB"
                     /locus_tag="ACGI57_000235"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFG9715326.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(7297..9633)
                     /locus_tag="ACGI57_000236"
     CDS             complement(7297..9633)
                     /locus_tag="ACGI57_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9715327.1"
                     /translation="MLDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGG
                     AENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDT
                     YDGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVL
                     DTTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSY
                     GVNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYG
                     YGNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTL
                     SRIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNL
                     TYWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINL
                     KEKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQL
                     LDVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNV
                     SHDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSG
                     DVYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENL
                     IINISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTE
                     GSGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(9605..10321)
                     /locus_tag="ACGI57_000237"
     CDS             complement(9605..10321)
                     /locus_tag="ACGI57_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9715328.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            complement(10735..11391)
                     /locus_tag="ACGI57_000238"
     CDS             complement(10735..11391)
                     /locus_tag="ACGI57_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9715329.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(11446..12075)
                     /locus_tag="ACGI57_000239"
     CDS             complement(11446..12075)
                     /locus_tag="ACGI57_000239"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9715330.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(12206..12847)
                     /locus_tag="ACGI57_000240"
     CDS             complement(12206..12847)
                     /locus_tag="ACGI57_000240"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFG9715331.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(12856..13527)
                     /gene="rpe"
                     /locus_tag="ACGI57_000241"
     CDS             complement(12856..13527)
                     /gene="rpe"
                     /locus_tag="ACGI57_000241"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFG9715332.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(13532..14416)
                     /gene="rsgA"
                     /locus_tag="ACGI57_000242"
     CDS             complement(13532..14416)
                     /gene="rsgA"
                     /locus_tag="ACGI57_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFG9715333.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(14514..15251)
                     /locus_tag="ACGI57_000243"
     CDS             complement(14514..15251)
                     /locus_tag="ACGI57_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFG9715334.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(15359..17374)
                     /gene="prkC"
                     /locus_tag="ACGI57_000244"
     CDS             complement(15359..17374)
                     /gene="prkC"
                     /locus_tag="ACGI57_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861606.1"
                     /note="PrkC, as found in Clostridium difficile, has a
                     kinase domain, two PASTA domains, and a C-terminal
                     Gly-rich repeat region; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing Ser/Thr kinase PrkC"
                     /protein_id="HFG9715335.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(17391..18143)
                     /locus_tag="ACGI57_000245"
     CDS             complement(17391..18143)
                     /locus_tag="ACGI57_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFG9715336.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(18163..19194)
                     /gene="rlmN"
                     /locus_tag="ACGI57_000246"
     CDS             complement(18163..19194)
                     /gene="rlmN"
                     /locus_tag="ACGI57_000246"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFG9715337.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(19202..20527)
                     /gene="rsmB"
                     /locus_tag="ACGI57_000247"
     CDS             complement(19202..20527)
                     /gene="rsmB"
                     /locus_tag="ACGI57_000247"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFG9715338.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(20589..21290)
                     /locus_tag="ACGI57_000248"
     CDS             complement(20589..21290)
                     /locus_tag="ACGI57_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFG9715339.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(21359..22111)
                     /locus_tag="ACGI57_000249"
     CDS             complement(21359..22111)
                     /locus_tag="ACGI57_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HFG9715340.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            complement(22121..23050)
                     /gene="fmt"
                     /locus_tag="ACGI57_000250"
     CDS             complement(22121..23050)
                     /gene="fmt"
                     /locus_tag="ACGI57_000250"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFG9715341.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(23065..23505)
                     /gene="def"
                     /locus_tag="ACGI57_000251"
     CDS             complement(23065..23505)
                     /gene="def"
                     /locus_tag="ACGI57_000251"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFG9715342.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(23523..26012)
                     /gene="priA"
                     /locus_tag="ACGI57_000252"
     CDS             complement(23523..26012)
                     /gene="priA"
                     /locus_tag="ACGI57_000252"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFG9715343.1"
                     /translation="MKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVIKIGHRILVPFGK
                     GNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWMKNRYLCTYIECI
                     NLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVEKVIDSKGKIKVD
                     KLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESDKIDDYIEQNKIN
                     VGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLEFKDYYREPKSIY
                     KSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVYMEIIEFALNQGL
                     DSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAVKAGKVRVLIGAR
                     SALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNITLILGSATPSIE
                     EYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSLKLQKEIRYAIEE
                     NNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRCHYCGYEKEIPKE
                     CPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGALDEILNKFKDKEA
                     DVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLITQVSGRAGRADKE
                     GKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNMLSVVVSGEDERLV
                     IKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQILFKDENIEINLLK
                     GIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(26287..27486)
                     /gene="coaBC"
                     /locus_tag="ACGI57_000253"
     CDS             complement(26287..27486)
                     /gene="coaBC"
                     /locus_tag="ACGI57_000253"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFG9715344.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(27491..27757)
                     /gene="rpoZ"
                     /locus_tag="ACGI57_000254"
     CDS             complement(27491..27757)
                     /gene="rpoZ"
                     /locus_tag="ACGI57_000254"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFG9715345.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(27760..28377)
                     /gene="gmk"
                     /locus_tag="ACGI57_000255"
     CDS             complement(27760..28377)
                     /gene="gmk"
                     /locus_tag="ACGI57_000255"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFG9715346.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(28407..29288)
                     /locus_tag="ACGI57_000256"
     CDS             complement(28407..29288)
                     /locus_tag="ACGI57_000256"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFG9715347.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(29292..30122)
                     /gene="dapF"
                     /locus_tag="ACGI57_000257"
     CDS             complement(29292..30122)
                     /gene="dapF"
                     /locus_tag="ACGI57_000257"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFG9715348.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(30409..31443)
                     /gene="ltaE"
                     /locus_tag="ACGI57_000258"
     CDS             complement(30409..31443)
                     /gene="ltaE"
                     /locus_tag="ACGI57_000258"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFG9715349.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            31739..33514
                     /locus_tag="ACGI57_000259"
     CDS             31739..33514
                     /locus_tag="ACGI57_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HFG9715350.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(33729..35381)
                     /gene="cplR"
                     /locus_tag="ACGI57_000260"
     CDS             complement(33729..35381)
                     /gene="cplR"
                     /locus_tag="ACGI57_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Contributes to intrinsic pleuromutilin, lincosamide
                     and streptogramin A resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein CplR"
                     /protein_id="HFG9715351.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(35680..36891)
                     /locus_tag="ACGI57_000261"
     CDS             complement(35680..36891)
                     /locus_tag="ACGI57_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFG9715352.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(37175..37708)
                     /gene="pyrR"
                     /locus_tag="ACGI57_000262"
     CDS             complement(37175..37708)
                     /gene="pyrR"
                     /locus_tag="ACGI57_000262"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFG9715353.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(38068..39015)
                     /locus_tag="ACGI57_000263"
     CDS             complement(38068..39015)
                     /locus_tag="ACGI57_000263"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFG9715354.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(38984..39430)
                     /gene="lspA"
                     /locus_tag="ACGI57_000264"
     CDS             complement(38984..39430)
                     /gene="lspA"
                     /locus_tag="ACGI57_000264"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFG9715355.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLVDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(39740..40519)
                     /locus_tag="ACGI57_000265"
     CDS             complement(39740..40519)
                     /locus_tag="ACGI57_000265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9715356.1"
                     /translation="MKKIICGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(40574..41056)
                     /locus_tag="ACGI57_000266"
     CDS             complement(40574..41056)
                     /locus_tag="ACGI57_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFG9715357.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(41322..42743)
                     /locus_tag="ACGI57_000267"
     CDS             complement(41322..42743)
                     /locus_tag="ACGI57_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HFG9715358.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(42889..43068)
                     /locus_tag="ACGI57_000268"
                     /pseudo
     CDS             complement(42889..43068)
                     /locus_tag="ACGI57_000268"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
     gene            complement(43081..43659)
                     /locus_tag="ACGI57_000269"
     CDS             complement(43081..43659)
                     /locus_tag="ACGI57_000269"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9715359.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGI"
     gene            complement(43664..44866)
                     /locus_tag="ACGI57_000270"
     CDS             complement(43664..44866)
                     /locus_tag="ACGI57_000270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9715360.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT        15066 a         7870 c         4856 g        17662 t
ORIGIN      
        1 atacaaaact tacttatact atttatgcta aaaacaaaaa atattttatt aaagttttta
       61 aaacttatgt aatataaaaa tctatactat agacttgatt atggaaagaa aattatcaaa
      121 atcattacaa ttagatatac tatctctaat atcattgtca tgaattatct ttaaaaggat
      181 atcataaatg gagtttattt ttatataatc atctttattt atagccaaca tcatgattaa
      241 tttaacagta gaattttcac tccaatatat tggttcattc aatatggaaa tacaaatctt
      301 agttcttata gagcttaatt ccataggatg aggtatagct aaaaaatcat ctatattggt
      361 agaagataaa ctttctctgt aaaatacaga ttcagcaaat gatggaaata caatttcact
      421 ttgagttaat ttgttgcaca tcaactttat aagctcatct ttatctttga ttttaggatt
      481 aacaataaat aaatttttat caaagaaatt gtccaaaaga ttactataaa tatgggaatt
      541 attagtgatt gacttagata tattctctat atcttttttt aatagtttta aatccactaa
      601 tacaatagga atactatcat gatttaaagg aattgtcgat attattatat ctacatctga
      661 caattcacta gctaaaaatt gattaaatga tagacattga agtatattta ttttatcatg
      721 aaaaactttg tttaattgaa cctctaataa cctacttgaa ccataccctg aaccacatac
      781 taatactacg tttttacact taatattact ttgaaaaaat ctctctattc cagcacctat
      841 atgcagggat acatatccaa tttcatcttc tgttaaagag taaatactat ttttaaaaac
      901 tttttctata gcatttaaag ttatttcaaa tgctaaagga taattactct ttattgtatt
      961 tataagggga ttcaccttat ttaaattata agatttagaa ttgagaattg atttaaagtg
     1021 cagtactaaa tcatcaaata atacactatc atttctaagg tcaaaagtat attgattgtt
     1081 gattacttct aaaatttctt caacatactc tataatttta gtatctaccc tattggaaac
     1141 gttatccaaa agagaacttt tagttataaa atgattatat agataaactc tgtctgcctt
     1201 tgaaataatt atagtatatt tactctcaat ataattaaat atattttcaa cagttgtatt
     1261 cgattgaaaa tctgttaaaa caacatctaa aacattatct aagcagtaac catcaagtat
     1321 tctacttaca gtaatagcta aatggataat aaagtttttt agattaaaat ctaggaaatt
     1381 gatattaaat ttattaattt cacttaacac taccctttga agctctatta aatctacatt
     1441 attaaataat tctatttctc tatctgtaat tccaattata tagttttcaa aattcctatt
     1501 tatcatatta ttagaaatac atcttcgaat atctatttcc ttaccttcaa ctttaactcc
     1561 ataatatggc ttagaaacta atattaaatt atactttgac aaaataggct ttattctttt
     1621 tatgtcattt agtattgtaa atttactcac atacaagcta ttagctaaat cttctagttt
     1681 tatatatgta ttgttataga gaatatattt taaaatatat tgatttcttt ctataggaga
     1741 atctggtatt tcacttgaat ccataatatc atcagatatt aatgctagat actgttggta
     1801 taatgctaaa ttaagaatat ctatataata tccttctcct ttttttattt taataatagc
     1861 accatttttc tctaaagttc catttataga agtaatatca tttcttatag ttctctcact
     1921 cacttttaaa tgattactta aagttttaca agtaatttta tcatcacttt catttaataa
     1981 tttaaacaac gtgagcaatc tcttgtgcat aaatatcccc ccttatagaa tttcttctaa
     2041 attatactat ttacactcat ataatacaat tactctaata aaattgtatt ataataattt
     2101 atttccactt ctatatggaa attagttatt atcataatat aaatttccat gtcgaagtgg
     2161 aaattagtta tatttaattt aattaggata atgattacaa tataaatata agttaagaca
     2221 ttatgaatgg agtggaaaaa tatatggtac tacaagaaaa atatatattt atagataaag
     2281 acttaaaaag caaagaagaa gtattaaatt ttattgcaga tagtgcttat atactaggta
     2341 ttacaactaa taaagaggct cttttaaaag accttctaaa aagagaaaat gaatttccaa
     2401 caggaataaa agatggattt gcgatacctc atacaaagag tgtaaatgta aaggaagctt
     2461 ccatactcta tattaaaaca actaacgagt tagaatggaa aactttagat gatagtaagg
     2521 ttagatatgt atttagtttg ttagccccac ataaaaatga aggaaatata catttgaaaa
     2581 ctctatctaa attagccaca tgtttaatgg aggatgattt tacaagtgaa gttaaaaatg
     2641 ctagtgatgc aagtcattta gtaaaatata taactaataa aatgaaggag gaataattat
     2701 gaaaatagta gctataacat catgtatagc aggattagca cacacaccta tggcagcaaa
     2761 agctttagaa caagctgcat caaaaatggg tcatgaaatc aaagtagaac aacaaggtgc
     2821 tatgggacga gtaaatacta taacaaaaga agaagcacaa gatgcggact ttatacttat
     2881 agcatctgac caaactatat ctggaatgga aagatttgat ggtaaaaaag tgataaaagt
     2941 caaaataggt atagcgttaa aaaaaccaga agctgtctta acaaagtgta tagaagctat
     3001 ttcttaataa aaaaatctca ctctaatata gaatgagaaa aatcaaaaat taaaaaattg
     3061 tctaatgtaa ttataacata ttatcaaaaa atataacaaa cttatatagg aggattaaaa
     3121 atgagtaaaa aaaataaatc tcttttaagt gaacttaagg gacacttaat gacaggtatc
     3181 tcatatatgt tgccacttgt aataggctca tctttagttg tagctatccc aaagcttaca
     3241 gctttagcag tgggtataac aagtttagat gcttataaaa atgtatctgg tttttatcat
     3301 attctttact taatggaaca agttggttgg actggcatag gtctattaaa tacggtatta
     3361 gcaggattta tagcattttc aataggtgag aaaccagcta tgggtgcagg ttttattggt
     3421 gggctcatag caagtaatac taatgcagga ttcttaggag cagtaatagc aggtttcttt
     3481 gctgggtatg tttgtaagtt tttaaagaaa aaaattaaaa tatctgggtc agcatcagga
     3541 atgatgccac ttataataat gccacttata acagttggat taactggttt tttaatgagt
     3601 gttgtattag caggaccttt aggaaacata aatacttcat taaatgcttg ggtagctgaa
     3661 atgtgtcaaa atggaacaaa ttcggtggta ttggctttaa ttttaggcgc aatgatagga
     3721 ttcgatttag gtggaccagt aaataaagca gcttggatgg caggaaatgc attattgtta
     3781 gagggaatct atctaccagc gatattggta aactgcgcaa tagttatacc tccactagga
     3841 tatggtttag ctacattcat aagaaaatca aaattttcca atgcattagc tgaaacagga
     3901 aaaggcaata tagttatggg tataattgga ataacagaag gtgcaattcc tttcacttta
     3961 acaaatccat taaaattagt tcctgttaat atgataggat gcgctttggg tgtaggatta
     4021 tcagctttgt taggtgtaca tgctataatg cctccagttg gtggattata tggatttata
     4081 tcaataggtt ctggatgggc ttacttagta ggtgcaatat ttggagcttt agtaatagca
     4141 atattatcaa caactttagt agacttcaat gaagatgaaa ctacggaaaa tacagaattt
     4201 gatgaaatag agttagatat aatgtaaatt aaaaacttaa ttttagataa ttgagaattg
     4261 agtgtactct caattctcaa ttaaattttg ggaggatttt tatgaaaaag acaaaagtac
     4321 atgtagttcc acacagtcat tgggatagag aatggtattt tacaacatca aggtcaaaaa
     4381 tatatttaat gaaagatttt caagatatat tagacatatt ggaagaaaaa gaagacttta
     4441 aatactttac tatggatgca caagcttctc tcttagatga ttatttaaaa tggagacctc
     4501 aggataaata tagaataaga gatttagttc aaagaagaag attagttatt ggaccttggt
     4561 atactcaaac agaccaattg gttatatcag gagaatctat aattagaaac ctttactatg
     4621 gaatagatag atgcaatgaa tttggtgaac ctatgagggt tggatatgta ccagattctt
     4681 ttgggcaatc agctcaaatg ccacaaatat acagaggttt tggaatagat agttcattat
     4741 tttggcgtgg agtttctgat gatatggtag atacaacaga gtttatatgg aaaggttctg
     4801 atggttcaaa agtattagca gttcaaatac catttggtta ttactatgga ggtaatatac
     4861 cagaagatga ttcagattta aaagaatatt taaaagatat aatcgtaaaa cttaaggtta
     4921 aagcttctac taataacgta tattttccaa atggatttga ccaagcacca ataagaaaaa
     4981 accttcctga tatattaaaa aaagctaatg aaatagataa tgaaaatgaa tacgaaataa
     5041 gtgatataga aaactatata gattcagtaa gaaatgaaag aaatgaattt acagagctta
     5101 aaggtgaatt tttaaatgct aaacatatga gaatacacaa atccatattc tcctcaaggt
     5161 cagaccttaa aataatgaat aacaaattag aaaattatat tacaaatgta cttgaaccgt
     5221 tattaacatt aagttattca cttggaaatg aataccctca cctagttata aaagatatat
     5281 ggaaacttat gtttgaaaat gcagctcatg acagcatagg ttcttgtaat agtgacacaa
     5341 caaatgagga tgtatacaca agatacaaac aagccagaga tttgagtata aatttattag
     5401 atttgcatat gagacttata agtactcaaa tcaaaaataa taatgaagaa ataacattaa
     5461 cagtattcaa tggattccca gaagtcagaa atgaagttgt agaatttgat acttatattc
     5521 caggtcaaga ttttgtgata aagaataaaa atggaaaaat tcttaattat ataataaaag
     5581 aaaaagaaga tattacaaat tatatattaa ctcaaacaat aagattaaac ccaagtaaaa
     5641 aaatatatat acccaaaact gtatatagag caaaaattgc actttcagta aaagatatac
     5701 caccaatggg atatactcaa ctagtttttg aattggatac taaaaataaa tgtgaaatag
     5761 aaacaagtaa ttgcaaggaa atagaaaaca aatactacaa aataacagcc aataaaaatg
     5821 gttcattaga aatattagat aaagaatcta ataagactta taaaaaccaa gctgtaattg
     5881 aagacaatgg agatgatgga gattcataca actattctcc tccaagaaaa gatatttatg
     5941 tttcatcttt agattctgta tcttcagtag aagtgctaaa atcaaacatt caagaagaaa
     6001 tgatacttaa atattcattg accataccag cttcactgga agaaagggct ataggtaaag
     6061 tttctgtgaa aatgccagta acaatgaaaa tcaccttaga acctaaagaa caaataatta
     6121 agtttaatgt taatataaaa aataaccaag cactatctca tagagtaaga gttttattta
     6181 atacagaaat agcatctaag ttttctatag cagatcaaca atttggaata atacaaagac
     6241 caacagtatt agaaaaagac cttgcattgt ggaaaagaga caattactca tggcaagaaa
     6301 aacctataac tatcgaacca atgcaaagtt ttgttacttt agaggatgga caaagaggaa
     6361 tagcattaat aactggttgt gtaagagaat atcaaatagt tggtgataat ttagatacaa
     6421 tagcattaac actattcagg tcatttggat atatgggaag agaaaattta ctttatagac
     6481 caggaagagc ttctggagaa aaaataatag caacaccaga tgcacaatta cttaaagagt
     6541 taaactttga ctttggtctt tacatatata acaataaatt tgatgaagca aatgtagcta
     6601 atacagctaa gagattctta acacctatac aaatatatga atatgctgat tttttaaatg
     6661 gtagacttat atttgcattc aatgacgaaa aacaaatata tgaaaatgaa tttagtttga
     6721 tgaatgtaaa taattcaaat ttcacagtaa gtgcaataaa gaaagaagaa aaaggagatg
     6781 gaattgtagt tagaatcttc aatggtatga aaaatgagga tgctaaggga agtcttaaca
     6841 tcaataaaaa tgtcaatgat gcttatagtg caatgctcga cgaaatctat gataatacta
     6901 gcaagcttaa agtaaatact aaaactgtag aggtaaattc tttatcacat tgtaaagtac
     6961 aaacaatagt aattaaataa tataaaatag cttatacaag atttcaaaat aaattgctag
     7021 taccatgtta atagtaaagc cagatattat gctatttata agctctgtaa cactaaataa
     7081 cattttgtaa aataaaaagg tgaaccttgt atattaggat tcaccttttt taaataaatc
     7141 aacattctgt tttaaaataa catttataag ctgttttgaa ataatgctta caaattattt
     7201 taaaacaata cctataaatt tttatttaaa gaatacacat aaatatttcc agactctttt
     7261 atcctataga ctttataatt attaaagaag tctaatctaa tttttgttaa aagtaattgt
     7321 aactttaaat aaatcaccat ccacacttat attcatctta ccaccctgta attctataaa
     7381 actttttgct atcgctaaac caagaccaga accttctgtg ttacgtgatt tatctcctct
     7441 tacaaatctc tccattatat catctacact aaaatcaatc tcagatgcag acatattttt
     7501 aagcataata tttactttat tatctgtttc taaaatatca atatacactc ttgaatttgg
     7561 cattgcatat ttgcttatat ttattataag attttcaaat actctaaatg tacgctgact
     7621 atctaatggt aatattattt tttcattaga aaagttattc tttactatta gagatgaatc
     7681 tgcaagtttg tcatctaatt ctaaaagtgt ctgcttcatt agagatacaa tatccacatt
     7741 tactatattc aaataaacat ctccactatt tgccttgctt acttcaaaca aatcttctat
     7801 tagatgttgc aatctttgtg actttctgtc taatgtatca atatacaact tacgattttc
     7861 atctgttata ctttcatctt ttaataaatc tatataagtg attatagatg ttagaggtgt
     7921 ttttaagtca tgtgatacat ttgatataag ctctgttttc atcttttgac tcttaacctc
     7981 ttcgtcaaca gcttttttaa atccagtttg aatatttcct aattcgtctt ttataggatt
     8041 aaaaaatcct aaatcttttt ccatatttac atctaagttt ccatctgaaa ttttctttgt
     8101 aacatctaaa agttgattat attcatatat tgtcttttta gagtaatctg acagtatttt
     8161 aaataatact actgaatata ttaatgcgag tattattcca aagaaccaca tactacacat
     8221 tataactata atcaataaat ttattcccaa aattgtaact atagttttat ttggcttttc
     8281 ttttagattt attgttctaa tttgattaat aactgttttt cctgcttttt ttagccatac
     8341 aaaaaacatt cctgtcacag tatgttttgt aaaatactct cttattccaa catttaaaat
     8401 atgttttaaa agtattataa atacaaatat tattgagaat attacaaacc aataagttaa
     8461 attcattaaa cttaccaata tactagccat tttagcactt atgtcattgt ttataaggta
     8521 ctctacataa ttcccactca aggtattact tattataaca gtaggatata aatctacaag
     8581 cccctgtata atagcaagag caataaagag tatctctaac ggtattcttg ataatgtatc
     8641 aaatcctagc aactctttag attttctata tgggaatgct aaagctacaa taacaactat
     8701 agctaatata atttttaaaa tcattccaga aatcttataa taagaattat tttctacatt
     8761 gcttatatat gaaattatac catcatcaaa ataagtattt ccataaccat actctacact
     8821 atcaccttta cttattactt taggtacagc atatactaat gttatatttt ttattggaga
     8881 taacttatat gaaattgaat ttcctcctaa acttgtatcc attaaatctt ctcttgcttt
     8941 agataacaac atttgattaa taattttttg atttactcca taagaatcca caatatttac
     9001 ttttccatct ttatcaaaat tcattacaac gtagaatcta tagttttctt ttatatctgt
     9061 aatctgcttt tgtctttcct catcattcat ttctgtattt tttgactgat tttcatcaga
     9121 attttctttt gaagcatcat tttttgttgt atcaagtact cctaattctt tattatcatt
     9181 aaaaaattca aaattgcttt ctttatcaac taaatagtac tttaaattct ttcctgacgc
     9241 tttaatgaaa tgctcccaat tttcaaagtt ttcattaaaa tctgcattaa tactatcatc
     9301 tgtatagtaa gtttctgaac catcataagt atctttttct ttacttacat tttttatata
     9361 gttcatagtt tcttcattga taaatagttt tgatggttgt atcatcttac cttcttgttc
     9421 ttccttcata ttatgatata aaacataact tcctttatat atactatcta caaagctttc
     9481 ctgttcaaat ggattctctg ctccaccttt agcacttgct tgtatagtag aatattgact
     9541 aatcatacct aatgatgctg ataacataag tataaaaact ataatagtaa ctatatattt
     9601 atctttaaat tttttcaatt ttgtatccaa caccccacac cacctttaaa tatttaggat
     9661 tttttgggtc aatttctatc ttttccctta tattcctcac atgtaccata actgtatctg
     9721 tatttacagc attctcattc caaactcttt catatatttc atcagcagaa aaaactcttc
     9781 ctgcatttct cataagtaaa cttagtattt taaattctat aggagttgtt tttatatact
     9841 ttccatcaat actaacttct tttgtatttt catttaattc taatccgcct attgcaaaca
     9901 cactatcgtc actttctacc ttttgctctt tatttccaac catgtttaga tacttagtgt
     9961 accttctaag ttgtgaatta actctagcca atagttctaa tggcttaaaa ggttttgtta
    10021 cataatcgtc tgctcctata ttaagcccca ttattttgtc aacttcctct gattttgcag
    10081 atagcattat tactggaaaa ttatagtttt ctctaagctt cattgtaagt gttataccat
    10141 ccattttagg catcattata tcaactatag ctaaatgtat ttcttggttt tctataacct
    10201 taagaccttc taatccattg tatcccttaa aaacattata tccttgattt aatagatata
    10261 tttctatagc atctgctatc tccttttcat cttctaccac caaaatatta tatgaattca
    10321 ttctaatctc ctttatatta atttataaat tttctttaca tatatgatta tacaatatga
    10381 tagaagtttt tccatttata tatatattta tatcttttgt tctggttatt tgtatttaat
    10441 gtccatctta gaaacctcct tctagtttaa atttaatatt ttttcatttt gttttatata
    10501 cttaatatta aaattaaaaa cttaagatat tacatatact tttctaaaga ttttctaaag
    10561 atttaaatat atttaaaatt aatatacaaa aaaagtagtt cttattgtat taattaaatc
    10621 aatgatttga aatataaaat taatatcaat aaaacctact ttttaatttt ttaaatattt
    10681 atttttaatt tattttgttt ttaatttact tcgattttaa aatgtccttg acttttattt
    10741 tataaatgta catatttctt ctactggttt tttagatgga ctcttcaaat tgtcctctgg
    10801 cactccaaga gctaacattg cacctaaaaa atactctccc ttttcaaaac cagtttctgc
    10861 ttctaaaaca gcttctatct catctgctgc atagttttga cttgtcaacc aacaagaacc
    10921 atatccaagt tcaattgctg atagtgtaaa gttttcaatt gcagcaccta gactctgcat
    10981 acctggattt cttataaata atttatctat agtctcctta ggtgcatcta taagctctaa
    11041 ttcataatat cctgatggat tatatacctt tgtaaaaact aatactaaaa ctggagcttt
    11101 taagtaaaat agtgtaaaat ttttaacaaa ttttctaaat ctattagcct tatccactga
    11161 tactttatcc atttctacaa gtatttcttg ttgcttttta gttatcacat ctgctatctt
    11221 ttccattaaa tcacggcgtt ttataacaac aaaatgccag ttttgaatat tcttgcctga
    11281 tggtgctgca cctgctgctt ttatcatttt taaaatatct tcatctgata catcttgatt
    11341 tttaaacttt cttacacttt gtcttttaaa aatagtatct tgtaattcca tttttaaatc
    11401 cccccataac ttttttatta ttttaatata atttttaaac tcatcttatt ttaatatatc
    11461 atgttctaac aaatctttaa atgaatctat aaaataatca tatacatcta cattcccaaa
    11521 cccatatttg gcatatataa aaggtatacc tacaaactta gcactatttg catcaccttg
    11581 cgtatcacca acatagattg gatttattaa tttatttctt tcaattataa gtttattatt
    11641 ttctccttta tgcaaaccag tattaccagg acattcaaaa tctataaaat actgttctag
    11701 tttatgtgct tttaaaaaag attctatata tccagcttga caattactta ctataaataa
    11761 tttatatttt ttagatagta atttaagagt atcttctata tcatcaaata atttagctcc
    11821 attttcagat aaatagtcac attctttcaa agaacattca ttcaataaat tcattctttc
    11881 ttcttcactt aaatttggaa ataaacgatt tgctatttcc tcaaaagaaa gccccataag
    11941 acttctaaat aaatcttttg tgacacttaa ttctatttcc ttatgctcac ttaaaacatc
    12001 ctgccagact ttgcatactt cttcagttga atcccacaat gttccatcta aatcaaaaat
    12061 aatactatct accacaaaaa atccccctta tgtcaaagtt tattatatat ttataattat
    12121 atactaaaaa gcagatacaa tcataaatat attttacttg attgtatctg ctctacaata
    12181 taaaaccaat tgtcaaaatc aaattttata aatttctaac tacaagtaca cagccttgct
    12241 ctactttaat cttacaagaa ttactcaaca taacatttga tattccaata ggtacagagt
    12301 atttcatata ataattatcg agagggtatt ctaagccagt aagtgttaca cccttagcat
    12361 caccatttat agctattaca gaaatagtgt cccctgggtt tccatacaat gaaatttcct
    12421 cattttcaac tatatacatc tcttctttat cagaaagtat cctagaatat ataccatctt
    12481 cttttaaata atataatagc tttatatttg caagagtgtg gtcaatcctt ccccctaaag
    12541 caccaaaaaa atcaatatga tttgctttaa gtgtcttagc taaaaaaaga caaagctctg
    12601 tatcagtctc atctttttta gatggaaatt tttcaaattt aacaccttta tttttataga
    12661 agtttatctt ttcttcttct actgaatcta aatctcctaa tatataatct ggcattattt
    12721 ccattttata agtgtgattt gcccctccat ctgcacatat tatgcagtca taacattcct
    12781 taattataat atctcttgta acttcgtaat cttcaatttc gccatttaaa acaatacaga
    12841 ttttcattat taaatttaaa ctaattcttc aagagaagca ttctttctaa ataaattaac
    12901 tgtttcttga atgtcatcac ttccaaatat tgcagaacct gctactatta cattagctcc
    12961 tgcttgtaca acttcggcta cgtttttagg gcttatacca ccatcaactt ctatatcaat
    13021 gtttaatcct tgactatcta taatagcttt taactcttta atcttatcta aaacacaagg
    13081 aataaatgat tgtcctccaa atccagggtt aacagacatt aataatacca tatctaaatc
    13141 aggtagtaca tgtttaatcg tatctactgg tgtagctgga ttaagtacta caccagcttt
    13201 tattccatgt gattttatat tttgtatagt tctatgtaga tgaacacaag cctcctggtg
    13261 aacagttatt atatcacatc ctgcttttac aaattcttca atatactggt ctggattttc
    13321 tatcataaga tgagcatcaa ataccatatt tatattttct ttctttaaac ttttgactac
    13381 aagtggtcct aatgtaatgt ttggtacaaa gtgtccatcc attacatcga tatgaagata
    13441 ttcacatcct gcactttcta cttttcttac atcttctaat aattttgcaa aatctgctga
    13501 taatattgat ggtgctaatt taatcatgtt tctaatacct cctactatta ttttgtctta
    13561 tttcatgtag cagctgtata taactatcat atctttcttt agatatttct ccatttgtaa
    13621 cagcttcttt tatagcacaa ttaggttcat tttcatgaat acactttgaa ccaaacttac
    13681 aatcattaaa tttatcaaat tctataaaat aatctttaag ttcaacttcc tctatatctt
    13741 caagggcaag ggaactaaaa cctggtgtat ctgcaaccat tccaccaaat tctaatttta
    13801 aaagttcagc gtgacgagta gtatgttttc ccctttttat tttatcactt acaactcctg
    13861 tttgaagttt aaaattttca tctatttcat ttaataagct tgattttcca actccagatg
    13921 gtcctgcaaa cactactaca ttttctttta attcttcttt tactttatct atatttaact
    13981 tagtaatatt gcttacagga atgactttat agccactaag ttcatatata tttttaacag
    14041 tttctaatgt atcattatca tctaaatctg cttttgtaag gattattaca gtttctaaat
    14101 tttctttttc tgccaaaact ataaatctat ctaaaagaga taagtttggt ttaggatttt
    14161 ttattgcgaa tactattaat gctttatcta catttgcaat aggaggtctt ataagttctg
    14221 tatctctact gtcgatttct tctaagatac cctttttttc attttcatca acaatgctta
    14281 ttttgactct atcaccaacc aaaggtgtta ttttctgctt tctaaatatc cctctagcct
    14341 tacattcata aataccatta taagtatcta cataataaaa tccactaatc ccctttataa
    14401 tttttccttc tagcatcaaa tgcctccctt tataaaattt atataaacac atactgttta
    14461 tgagtagtat atcgtgttat tctttcctat ttttatataa taccacattt tttttaaata
    14521 acaaatcctc cgtttggact aattacctga ccagtgataa aatttgattt gtctgatgcc
    14581 aagaaaatag cacaatttgc tatatcttct ggagttccta gcctcattag aggggtttct
    14641 tccacaagag cgtctatatc ttcctcatta tatccagata acatgtcagt attaatgaca
    14701 cctggagcta tgctatttac tctaatattt gatggtccta cttctttagc taatgccttt
    14761 gtcataccta ttatgcctgc ttttgttata gaataatgca cttcacaaga cgcacctgat
    14821 ataccccaga ttgaagatat attaattata tttccttttt tttctgatat catgtatttt
    14881 aaagcaactt gtgaacaata aaagcttcct tttaaattaa tattcatcat attatcccaa
    14941 tcttcatcag ttatatctgt aaacaactta tcttgactta caccagcatt gtttacaagt
    15001 acatccaatc ctccaaattc ttttatacaa taatctacca tatcctctac atcttctcta
    15061 tttgaaatat cagccttaaa aagttttaca gaaaaatttt tttcatttaa tatagtgtac
    15121 aactctttag cttcattttc tgacttatta aaatttacta atacattgta accttctttt
    15181 gcaaaagctt tagacatagc tttaccaatc cctctagctc ctcctgtaat taaaactgtt
    15241 ttcttcttca taaatacttt tctccttata ttataaccaa ttcagtattt tatacaaaac
    15301 aaactgccct ttaaagaaaa tattttctat aaaaggacag ctcttatttt attaatattt
    15361 attctgtttt accaatgttt gcgccagttc ctgatgaagt tcctccatta tctcctgagt
    15421 ttgagcctcc tgaactatct ccattgtctc ctgagtttga acctcctgaa cttcctccat
    15481 tgtctcctga gtttgagcct cctgaacttc ctccattatc tcctgagttt gagcctcctg
    15541 agtttccacc actatttcct gagtttgaac ctcctgagtt acttccatta ttgcctgagt
    15601 ttccaccact atttcctgaa tttgagcctc ctgaatttcc accactgttt cctgagtttg
    15661 aaccaggttc tgttggtgta ttattctctg gtgttgttgg ttttttatct ggtactgttg
    15721 gagtctgtgt atttgatttt tctgaacctt tgcttacata taggtttacc tcatcacctt
    15781 cctgagctct tgatgagcca gcacccggac tctgacttaa aactgtacct tctttatata
    15841 ttgagctgta ttcatactta acagttccca cttttaagtt attttcttca ataagttgtt
    15901 ttgcattact caaagtaagt cccactacat taggaacaga tgcctgactt gaacctttac
    15961 ttacgacaac atttatagta tcaccctttt tgacatttgt acttccacct tcaggtgtct
    16021 gtgatattat acaattttca tcaacttcac tattatactc ttcacttttt acactcaggt
    16081 ataatccttc tttttcaaca gcactttgag cttcttctaa tgtcatattt acaagattag
    16141 gaacagtcaa agatttattg ccaaaccctc cagcaaataa aaacttatat gctaagaata
    16201 cttgagcaca caatatcagt atcaaaacag ccgcaattac ttttaatctt cttctagatt
    16261 ttgggctttc ctgttttttc ttctttttct tagttctctt acttggagta ctcttaggtc
    16321 tctgattctt tttagctctc attatctctt cctcttcttc atcttcatca tcatcttctt
    16381 cataaaaatc atcatagtaa tcttcatcat catccaaatc agctacttca acaggcttaa
    16441 ccactttttc aggagctggt tttgcaagtg ttggatttac aactttatta atttcttttt
    16501 catcaatttt cttagttgca aaatcatcat attcttttat aaaatctaaa tcaatatttt
    16561 tttctatata ctctatatct tctatcaatt cttcagcagt ttgatatcta tctgcactag
    16621 atttttctgt aagctttttt attgtagttc ttacactttg aggaatccta actttttctt
    16681 ctgatgtaaa gtctatatca tcattaatgt gttgaagtgc tatagaaatt ggactatctc
    16741 ccctaaatgg aacttttcct atcaacattt catataaaac tatacctaga gaatacaggt
    16801 cagcattatt agttacaaat ttacctttag cttgctctgg tgaaaaataa tgcactgaac
    16861 ctattatact accaatatta gtcattgttg aatttgaaac agctttggct ataccaaaat
    16921 cagccacttt tactactctt ccttcatttg atatgagaat attatgaggt tttatatctc
    16981 tatgtataat cccctttttg tgcgctgcac taagtgccat agctatttgt tttgttatat
    17041 ctaatgcagt atattcatct aatatacctt catcttgtat aatctctttt agattcttcc
    17101 catctacaaa ctccattaca atgtagtgaa cttttccatc ttcaccaaca tcataaacat
    17161 ttactatatt tggatgtgaa agacttgcta ctgcttctgc ctctctttta aactttgtta
    17221 gaaattcatc atcatctaca aattcaggtc taagtacttt aagtgcaaca gtcctgttta
    17281 ataatctgtc tttagcctca tatacaaagg ccattcctcc atcaccaatc ttcctgatga
    17341 tttcatatcg atttcctaaa attgtatctc ccacaatatc accgtcctaa ttatactttt
    17401 atcactataa ccgaaacatt atctttacca gaaacatcat tggccatact tatcaattct
    17461 tcacttatat tactagttct ctcatattca taatctaata ttaaatctct tatatcttca
    17521 tcatcaataa acccagttaa tccatctgtc gcaagaagta ttatatcact ttttttgatt
    17581 tctttcttaa aaatatctac aacaaccata tcatctgttc caattgctct agttattcta
    17641 ttcctctgtg gatgtcttct tgcctcttct tctgtaatga catgggccat cataagttct
    17701 tcaaccacgg aatggtctat agttatttta tcaaattttt catctgttaa caaataacat
    17761 ctgctatctc caacatttgc cacatataag acattattat acactatagc cgtaactaat
    17821 gtagttccca ttccttcaaa ttctatatct tccatagatt ttttgtgcac aattgagttt
    17881 acattattat aagcctgttt taatatatca tctatatagt ctatttttac attatcatgt
    17941 tgtaataagt tttcttttag aaaatcaatt atattttcta ctgctaattt actggcgact
    18001 tcaccttttt tatgtccacc cattccatca gctatggcaa atattcctat tggaccatgt
    18061 tctgtatcta taacttctcc ctcacaatag tcttcattgt tttttcttat ttttcctata
    18121 tgggaggcac aactataaac catacgactt cctccttaca aattacttgt attttcttct
    18181 caattgccca caagctccac taatatcaga acccatagac atcctaactg tggcaggtat
    18241 attgttcttc tccaaactat ccctaaattt ataaataaat gctttatctg gtttttcgta
    18301 ttctctttct tcaactttat ttattggaat taaatttaca tgacatagca taccttttag
    18361 gagcttagct aatgcctttg cttctttttc tgagtcattt actcctttta taagagaata
    18421 ttcaaatgta acccttctat ttgtcttttt tatataatat ctacatgcat tcaatatttc
    18481 ttcaatagaa taagcatttg ctacaggcat aatttttctt cgttcttcat catatggtga
    18541 atgaagtgat aaagctaaat ttatagctat ttctaagtct gccaactcat acattttagg
    18601 tactattcca caagttgaaa gagttatatg tctataccct atattaagac catttttttc
    18661 gttaattatt tttaaaaatt gttttgtatt ttcaaagtta tcaagtggct caccactccc
    18721 catcaataca agatttgata ccctcttacc agtatcttct tgaattttaa taacttggtc
    18781 taaaatttcc catggctcta agtttctaat taatccatcc attgtagatg cacaaaaatt
    18841 acatcccatc ctacaaccta cttggttaga aacacagaca gtaactctac tatcataatc
    18901 catcatcaca gtttctatta tatttccatc atctaataaa aacaaatatt tttttgtatt
    18961 atctacctta gattctaact ttaattctat atctatatgc cctatacatg aaacctcttc
    19021 taatttattt ctcaaacttt ttggtatatt gttcatatca tcaaaagttt ttgcaccctt
    19081 atatatccaa gagaatattt gacttcctct aaagggcttt tcatctatag ttttcataaa
    19141 ctctttaagt tcatcttcag taaaattttt taatactatc tttttctttt ccattctctc
    19201 actaccttat tctctttaat tttgctatga aaaaaccatc tataccatga atattaggat
    19261 atatctttaa atatccttta tcttgattgt ctaagtctac atttactgta tcaattggaa
    19321 caaattcaaa gttgtgattt tcttcaacaa aagaagtaac tatatttata ttctccacat
    19381 cttgaattgt acatgtacta tatactaaag ttccaccaac tttgacatat ttagatgcat
    19441 tttctaaaat tttcttttgt atagaagtga tgtcttttaa ctcttcttct ttcttatact
    19501 taatttctgg tttacgtctt attataccaa aaccagagca aggaacatct gctaaaacat
    19561 aatcaaattt acttatacta ttttcatcaa tctcagaagc atcaaatctt tctacaaaca
    19621 catttttaag ccccaatcta tttacagtag agtttataag ctttagttta tgctcaaaaa
    19681 tatctctagc tactacttga ccagtattgt tcattaaagt agctaagtga gtagatttcc
    19741 ctccaggagc actacataca tccaaaacaa atgaaccttc ttttggatta attactttcc
    19801 ctactatcat agaacttata tcttgaattg taaataaacc agccttaaat aattcatttt
    19861 tttctatatt tttcattttt tcgactttaa tagcttcttc taccatagga acttttaaac
    19921 atacaatacc catttcactt aatttttcaa taagttcttc tctacttatt ttaagtgtat
    19981 tcgttcttat atagatacta ggcttttcat tatttgcttc caacaaatct tcagcaaatt
    20041 cttgtccaaa tttatctatc cagtttttta taatccaaga gttataggaa tactttatag
    20101 ataagtattt tacactatct tcctcagtta tgtccattat agtctcttta gctcttattt
    20161 catttctaag aatagcattt atgaaaccag aagatttttt atcatatttc ttagataatt
    20221 ttacagtttc attaactgct gcataatctg atatactatc taaaaataaa atctgatatg
    20281 ttcccattct taaaaagatt ttaacataag ttgacatttt ttttaccttt atttttgaaa
    20341 gtttattgat tatataatct aaataatatt tattttctat tactccataa attaactctg
    20401 ttgcaagtcc tctatccata tcacttattt ctaaattttt aaaatgctta tttattgcaa
    20461 tatttgaata attgtcattt ttttctatat cacataatac tttaaatcca acttctcttg
    20521 catccatata aatttctccc ttttattaaa cataaaaaag ggttgtttct aaacaaccca
    20581 ctttactttt agtcatttct tctttgagct attagtaaca atctaagtaa ttgaagtatt
    20641 gaaaccaatg ctgctgcaac atatgttaaa gctgctgctg aaagtactct tctactttgt
    20701 cttgactcac tttcatctat aatccctaaa ttcccaagtt gaacgattgc tctacttgaa
    20761 gcattaaatt ctacaggcaa tgttattatt tggaataaca ctgaagccga aaacaatagt
    20821 ataccaattt ttaaaaatgg accagccata ataaatccta aaaatattaa aaaccaagat
    20881 atacttgaag caaaattcac tactggtact aaactacttc ttatttgaag aggtgcataa
    20941 cctttagcat gttgtatggc atgaccacac tcatgtgcag ctactgcaac agaagttata
    21001 gatgttccat aatatacatc ttctgacaac cttacagcct ttcttcttgg gtcataatgg
    21061 tcacttagat gccctctaac catttctatc tttacatcat acaaaccatt tgaatcaagt
    21121 actcttcttg ctgtttgctc tccagtatat cctcgacgtg tatttactct taaatactta
    21181 tttgttgttg aactcacttt aaattgtgca tatatagtaa gtaatatagc tggaattaat
    21241 acaaccattg ttgggtcaaa tccccaaaaa ccgccatata cagggtacat aaataacact
    21301 ctcctaaaat atatttttat tatacatcct cttaatattc acttcactta aaaacagttt
    21361 aattacaaac taatatattt tttcctgtta aaaagcctat agctttttca atatccataa
    21421 tgtctacatt agtatttaca caaggtccat taggtctttt gttaaataca cctaaaacag
    21481 gaattttctt aatatcttgt attccagatg tcaaatctct ttcacatgca acagctacaa
    21541 cagctttagg tttattatcc attataattt ttctagctag ggttcctcca gtagctacaa
    21601 atatttttac attagttttt tcctttagct taattaattc tcctatgttg catcttccac
    21661 attctttaca attatctata tcattagtaa cttttaattt acaactattc tcttgaatac
    21721 aatgaggtat taaaattatt atatcctctg gattaaaatt atatttatta ctgtatatat
    21781 attcattatt taactttata tatattcttc taatatcact ttttgacata ccaaatgatg
    21841 atgcaatgaa agatataata ggaaataatc cactgacaat attaaaattt attttcatta
    21901 tatttgagtt aacatacttt cctttaatta ctctataagt aactgctgtt gaacatagta
    21961 ttataagtat tagtgatatt attataatat ttatcatcaa ggagaatatt tgagttaaac
    22021 ttattataaa aaaattaaca actcctatca aaattcctaa tattaaaatc aaaatcccaa
    22081 cacttgttaa atacttcttt atatctgtca tacttttcac ctaacccaat actaaaccag
    22141 tttctatagt attccctttt atatattcac caaccaacat tctcttttta ttcggcatct
    22201 gaatttcttc aattaaaagt acattatcta tagtacttac ttttatccca tctttgctta
    22261 catcaattat tgtacctaca tcttttgtgc ttttttcatc taacacttta gttttccaaa
    22321 ctttcattat gacatcatta taggtagtat aggcacttgg ccatggattt actcctctaa
    22381 ctaaattgtg tatttctctg gcactttttg aaaaatctat gttacctaat gatttattca
    22441 ttattggcgc ataggaaaat tcttcatgat tttgtacttc tcttggagca tttccttctt
    22501 caattaatct taatgtttct tttaaagttt ctgcgcctat attcatcatt ttatcatgaa
    22561 gttctcctgc tgttatattt tcatcaagat ttacttctgt ctttagtatc atatctccag
    22621 tatctagccc ttcatccata tacatagttg taacaccagt cttttcttca ccatttatta
    22681 ttacccaatt tataggtgcc gcacctctat attttggaag taaagaaaca tgaacattta
    22741 tacatccaag tttaggaatc tctaatattt cttttggaag tatctgtcca aaagctacaa
    22801 ctactattac atctggattt aaagatttaa ttttatctat aaattcttta tctctagcct
    22861 ttactggttg atatacaggt atattatttt caattgccag ttcttttact ggactcatac
    22921 ctagtttttt acctcttcct tttggtttat ctggttgagt tacaacaccc aatatttcat
    22981 acttctcatc tattattttt tgtaaacatg gaactgctat atcaggagtt cccataaata
    23041 ctattttcat caaatcaact cctactactt ttctattttg tcaacaaaca aaattccatc
    23101 taagtggtct atctcatgac aaaatgctct agctaaaagt tcttctccct ctaactctat
    23161 tgtttcacca tttctattta aagctctgac ctttacataa tttggtcttc taacatttcc
    23221 cgcttctcca acaacactta aacatccttc ctcatctatt tgctccccag atgtttctat
    23281 tatttcagga tttataagtt ctataagctc ttctccaata tctattacta ctactctttt
    23341 taatattcca acttgaggtg cagccagtcc aacaccatct gcatcataca tagtttctgc
    23401 catatcatct aataattgta ttatcttttc atcaattttt tcaacctttt ttgatttttt
    23461 tcttaatact ggttctccta tttgaactat ttgtcttaaa gccattataa ttcctcctaa
    23521 atttataata cactgtttgg attaatatct atagatacat tgatattctt atcaaatatc
    23581 aaatctcgtt ttgttataca tatatatttt attataccct ttaagagatt aatttcaata
    23641 ttttcatctt taaagagtat ttgccatcta taattttgat ttatttttga tattgaacat
    23701 ggatttggac ctaatataaa tcctaaatca tttatacccc ttttttctaa taaataaatc
    23761 aatgatgcat gcatattttt tatatttttt atcactagtc tttcatcttc accactaaca
    23821 actacactta acatattatt aaaaggagaa tatccaaatg cttttcttat ttttatctca
    23881 tcttcataaa aaccctcata atcatattca atagcatgct taatagcata gtgttcagtg
    23941 tcataagtct gaagtactac ttttccctct ttatcagctc tccctgctct tccagagact
    24001 tgagttatca attgaaatgt agtttcaaaa cttttaaagt ctggaaaatt taatatcatg
    24061 tctgcagaca aaatacctac taaagttaca ttctcaaaat ccagcccttt acttaacatc
    24121 tgtgttccaa taagtacatc tgcttcttta tctttaaatt tatttagtat ctcatctaaa
    24181 gcaccttttt ttgaagttgt atctttatcc attctcaaag tttttatatc tggaaaaata
    24241 tattttaatt cctcttcaat tttttgagtt ccaactccaa atggcttaac ataagtactt
    24301 ttacattcag gacactcttt tggaatttct ttctcatatc cacaatagtg gcatcttcca
    24361 gtattgcttt tcttatgata agttaaagat atatcacaat tttcacattg aaacacatat
    24421 ccacactttc tacaagatac aaaatttgca taacctcttc tgtttaaaaa tagtattact
    24481 tgattatttt cttcaatcgc ataacgtatt tctttttgta atttcaaact aaatatactt
    24541 ctatttcctt tatctaactc atctttcatg tctaccactt caatgtttgg aagaggctta
    24601 tcatttgctc tactttttat atttataagt ttgtattctc cagattttgc cctataatac
    24661 tcttcaattg agggcgttgc tgaacctaaa ataagagtta tattattttt taatgccata
    24721 aatctagcaa cttctatagc attaaatttt ggattcttct cagacttata tgatgactca
    24781 tgacattcat caattattat taagcctaag gaattaaaag gtgcaaataa agctgaccta
    24841 gctccaatta acactcttac tttacctgct ttgacagctt tgtaaacatc atgtttttct
    24901 ccttcagata gtttactgtg gaacactcca accaaatctc caaacctact ctttagccta
    24961 tctattgttt gaggagttaa tgctatctca ggtactaaga atatactgtc taagccttga
    25021 tttagagcaa attctattat ttccatgtaa acttcagttt ttccacttcc agttactcca
    25081 tgaattaagt atggtttttt atcatctaca aacatattcg actttatctc atcaactgct
    25141 tcttgttgct cattgtttaa ttttatactt tttagtacgg atttgtaaat actttttggc
    25201 tctctataat aatccttaaa ttctagggtt attagctttt tcttacttag agaatttata
    25261 ctttgttttg aagcatttaa taaatccaat aaatcattta tctctacatt ttcattattt
    25321 ttaagaaaac ttaatatctc tttttgttta cttcccacat ttattttatt ttgctctata
    25381 taatcgtcaa tcttatcact ttctaaactc aaagatatat aacatacctt cttttcattt
    25441 ttgtgatttt tatattccca acataatttt ataattccat tcttattcat tgtgtacaaa
    25501 gaactattta agtttggtat atattttaac ttatcaacct taattttacc tttactatca
    25561 atcactttct ctactatttc tctattttta tcacttaaag ttgatatttt ttccttcaat
    25621 tcaatatcat ttaatccact taaactttca cctaatagga ctactttata attatttaat
    25681 ttgtatccct ttggatatat caaatttata cattctatat aagtacacaa atatctattt
    25741 ttcatccaat aaaccaactc taaatcttct tttctaaata tcggattttc atctaatata
    25801 tcaataattt cttttatttt tattttttca tctaagctat ctgtaaattg aaatacaaag
    25861 gcttctgttg gtttattacc ttttccaaat ggtactaata ttctgtgccc aatttttatt
    25921 acatcagata aaaatacagg aatttgatat gtaaataaat tatctgtata tatagtatta
    25981 cttctaacta ttacctttgc atattttttc attataaaac ctctacttaa atttaatata
    26041 ttgttcatat tttaatatac tttacaagat attaaaatct cagaatttat aagcaatttc
    26101 aagttattaa tattaacttt attttaataa ttaactatta attatatttt atactttatg
    26161 acaaaataaa tgctgaaaac tacaatttaa ctgacatttg cgagttttta ctcgctaaaa
    26221 ttgaattgca gctttcagcc actttagctg tgataaaata cacaaatatc atatttaaaa
    26281 aaggtcttat ttctcaagca acgctttaac tttatctaaa ataacattgg ctacttcttc
    26341 ttttttcatc ttaggatact cagtaatatt tccctctaca tctattattt ttacaatatt
    26401 agtatcagct ctaaaaccag caccttcttt agttaaatca tttgccacaa taaaatctaa
    26461 atttttcttt tttatttttc ctttggcatg ttctataagg tcattagttt cagctgcaaa
    26521 tccaattagt atcttatctt cttttatttt tcctatttca taagcaatat cataatttct
    26581 acttagttct attgataaat catcattctt ctttttaatt tttttgtcag aataagtttt
    26641 tggtttataa tctgcaactg ctgcactttg aattactatt ttattttcac ttaaatttga
    26701 tactactgct tcatacaaat cttgtgctga ctctatttga acaaattttt taagattttg
    26761 tggaatagaa agatttgtag gacctgatat taatgttaca tcagcacctc tagctatagc
    26821 ttctcttgct atagaatatc ccattttacc agaagaccta ttagttaaat atcttactgg
    26881 gtctatactc tctacagttg gaccagctgt aactatcata ctttgacctt ttaaatcttg
    26941 ctctttagta agattagtca aaacttcctc tactattata tctggattag ctagtttacc
    27001 tgttccaata tctccacatg caagccttcc actttccggt tcaataaatt tatatccaaa
    27061 gctctttaaa gtttgtatat ttttttgaac aattggattt tcatacatat tagtattcat
    27121 agctggtgct ataagaacct gtgcttttgt tgccataaca gtagttgtca acatatcgtc
    27181 agctatacca tttgcaatct tgcctataac atttgcactt gctggcacta ttaagaataa
    27241 atctgctttt tttgctaatg atatatgttc aacttcccaa gtttttggtt cttcaaacat
    27301 atcttctact acataatttt gactcagcga ttgaaatgta agtggtttaa caaattctgt
    27361 tgctgatttt gtcattataa catggatatt tgcatttagt ttttttaact tacttacaac
    27421 atcacaagcc ttatatgcag ctataccgcc acttatgcca attacaacag ttttatcttt
    27481 tagcatgacc ttactcctct tctgaaattt gttggtgttg ctcagcatga tgtctagctt
    27541 cttctatctc tatttcctct tctgtcaata atctatatgt tatttttcca cttgcaactt
    27601 ctttagttgc tacacataca ggcttttcct tagttttatt tgaaacatat ggttcttctc
    27661 catcaattaa ttttcttgct ctctttgaaa cagtccctac taaatagtat ctgttgtcta
    27721 ttttttctag tacctcattg atagatggtt ttaacataat tataattcct ccttaaattt
    27781 ctctattata atatttttat atctgctaac tttattcttt tcagatgata ttataccttc
    27841 tatttcttta gcagatgttt ttacatcttc attaaatata aaataatcat aatccttaat
    27901 cattttaatt tcttcatatg cacagctaaa tctcttctct atttcagctt ctgtttctgt
    27961 acctcttcca acaattctat tttttaattc tttaagtgat ggtggtagta caaatataaa
    28021 tactccctct gggcaaactt cttttatttg ttttgctcct tgcatttcaa tctccagtaa
    28081 tacatcttgt cctttttcta aacattccat tattgccgct tttggagttc cataaaaatt
    28141 atcataaatt tgagcatact ccaaaaactc acctttttct atcatctctt caaacttttc
    28201 cttacttatg aagaaataat ttactccatc aacttctcca gttctgggtt ttcttgtagt
    28261 agctgataca gaaagtttta ttgtatcatt ttctttaagt agttctttgc aaatagtacc
    28321 ttttcctgca cctgatggtc ctgatacaac tagcaataat ccttttttca caaacattta
    28381 agcattcccc ttctaatatt gtattcttac tctatatttt gtatttgttc tcttattttt
    28441 tccaattcgc tttttacttc tactactaaa ttagttatat ttatatcaga tgacttcgaa
    28501 cctatagtgt tagtttctct attcatctct tgtattaaga aatctatttt tctacctata
    28561 gagtcgtctt taaatatagt atttttaagt tgccctatat gacttttaaa tctaactatt
    28621 tcttctgtta tactgctttt atctgcatat attgcaactt cttgtgctaa tctattctca
    28681 tctattatac ttgggtcatc cagtaattca cttattctca aattcaattt ttctctataa
    28741 tcttctacaa cactacttga atatttttca atctcttcta tatgattttt taataaatca
    28801 catctcatgg caatatcttc agctaatttt ttaccttctt cacttctcat ttctcttaat
    28861 ttgattaatg catcttcaac tgcttgattc aacattgacc acaataaatc ttcatcttct
    28921 tccttttctt ctattttcac aatatcagga aactttgcaa cattcataac gctaatatca
    28981 tctataatat caaatttatt ttttatttct tttagtatgt ctatatactg agatgctaat
    29041 ccctcatcaa aatttaagtt tacatcttct ttgcctaata agtcaaactt tatgtataaa
    29101 tctactcttc ctctttttac ataatttttt actaaatttc taactttatc ctctaaaaat
    29161 gaaatttttc ttggaagacg tatgtttata tctgaatact tatgatttat cgttttacac
    29221 tctactaaaa agtaataatt atcatccttg tattctcctc ttccaaaacc agtcatactt
    29281 atagccatta attaaacctc caaaatacct tcacatattt ttactgctgg acctgtcata
    29341 taagagaaat ctcctacatc tattttaact gttccacctt gagacgttac atttacacta
    29401 ctttctacca tattaagtaa attgcacact attgctgatg cagtcatacc agtaccacaa
    29461 cctaaagtat atccacaacc tctttcccaa gtataaacat gtatattata cttatcttct
    29521 acctttacaa aatttacatt cgttttttca ggaaatattt tattattttc aatctctttt
    29581 ccatatttac aaacatcatc tacatctaat tcatctacta taactactgt atgaggaacc
    29641 cccatcaaca tagaagtaac tttaagctcc ctatctaaaa cctttatact ttcttgcaca
    29701 aaacgttctt tatttgtaag tacaggaacg tccttagcta taaaacttcc ttttcccata
    29761 ttgactctta tagagtttat ttctctatta gtaagattta cttttatatc ttttatacca
    29821 tctaatgtat ctactgtaaa actttctttt ttaactatac aattatcata aacaaattta
    29881 caaaaacatc taaggccatt tccacacata gctgctcttg aaccatcaga attataatat
    29941 accatttcta catctgcaac ttttgaatct ttcacaacta acaatccatc cgctcctaca
    30001 gaaaatcttc tatggcatac tttttttgct aaagcagtat aatcagatgg atttatctgg
    30061 tcgaatcttc catctattgc tataaaatca tttcctattc catgtagttt ccaaaatttc
    30121 ataagtatcg ctccttcgta aattaacact taaactatta taacataaaa aaattcccat
    30181 aaacttataa tttatgggaa aagttttgta atgacaacta taatttgagg atgtgaggga
    30241 gagttgccat tacaattagg gtaattatat tatgttttta agctagttct ttctatgttt
    30301 atatattaat atttctatat gaattttatg tatgttttat ttaaactgtc tatgaatttt
    30361 ttaattaaac atctggtaac tagctataat ccttctagta gatatctatt aacatacacc
    30421 tattaattct ttaagtgttt ttaaaacata gtctaaatct tccttagtaa tatctacatg
    30481 agttacaaat ctatattcac cattttccat tttattaatt tttatatttc tttcaaaaaa
    30541 ttcttttatt aaaacatcct cttttataat atcttcactc atcttaaaaa ataccatact
    30601 tatatctgga ttaacattgt ttacttcaat gccatcaatt ttatttaatt cacaagctaa
    30661 atattttgca ttttcatggt cttcattcaa tcttaaagtc attttttcaa tggcaattat
    30721 accagctgct gcaactatac ctacttgtct cattcctcca cccataactt tttgctttct
    30781 tctagctttt gctataaatt ctttatttcc agcaagtata gaacccattg gtgcagctaa
    30841 accttttgac acacagaaca taacactatc acaacacttt gctatttctt tagcttctac
    30901 acccaaagtt aatgctgcgt taaataatct tgctccatct aaatgtactg gtaagttatg
    30961 tttctcagct atttttttaa tttctcttaa attatcaaga ctcacaactg ttccacctga
    31021 atatgcattt tctacacata taagccctgt ctgtggttca tgtatgtcat ctcttctaat
    31081 tgccttttca acaagttcag gatttagagc tccactttcg tcttcatcta tagttctaag
    31141 ctgaactcca gctattacag caggtgcccc aacctcatga gtcacaatgt gataaccttt
    31201 accaataatt atttcttgcc ctcttaggca atgagtaaat agagctaatt gattcccaaa
    31261 agttccactt ggaaggaaaa tagcatcttc ttttccaact ttagctgctg ctaattcttc
    31321 aagtttattt acagtagggt catctctaaa gattgaatct ccaacaggag cctttgccat
    31381 agcttctctc atttcgtcag taggcatagt aacagtatca cttcttaaat cgataaatct
    31441 cattttttca accttctttc atatttatat actatagaac cttaaaattg attttttctg
    31501 taaagttcat atactttcat agcttatact ctacacaata attatactat atttacttta
    31561 tatttaatct actttaaatt attaatttta aaaataaagt ttcctctcta ataaatataa
    31621 ataattatac aatttaaata ttaatctcta ttgtttaaat tccccctagt cacaaagcgt
    31681 atattatgtg tataattaat ttactgactc attttggtca aagaaagggg tgctataaat
    31741 ggcattagat ggattagtta tacattcaat agtcgatgaa ttatcatcaa aacttacagg
    31801 tggaaaaata gataagatac atcaacctga agatgatgag gtaattttta atataagaaa
    31861 taataaagaa aattttagat tggttttaag tgcaagtgcc tcaaatccta gagtttatct
    31921 tacaagtaat tatcaaaaag aaaaccctct aaaagcgcct atgttttgta tgttacttag
    31981 aaaatacata caaggtggaa atatagtaga aatttctcaa ataggttttg aaagaataat
    32041 aaaaataagt gttgaatctt tagatgaatt aaaagaaaag acagttaaaa atattatgat
    32101 tgaaattatg ggaagacata gcaacataat aataactcat ggtgaagaaa ataaaattat
    32161 agattctata aaaagagtcc cttttagcat aagtagagtt cgtcaagttt tacctggtca
    32221 tgattattca ttgccaccag aacaaaacaa actcaaccct cttgatgata tatctaagga
    32281 tttgtttatt aaaaacctag aggaattgga aggacctatc tttaagtcta tatattctaa
    32341 atttctaggt ataagcccta ttatagctaa agaaatttgc tacagagcag gtgtaaatca
    32401 aaatgctatc ataaaggata tatctgatga acaatttgat tctttacaca aagtattttg
    32461 taatttattc aatgatataa attctaacaa atactctcct tgcattatta tagataaaaa
    32521 ggtggataag gtagttgatt tcagttgtat aaaccttaca ttatttagcg atttatcata
    32581 tataaataag gatagtatgt ctcgtattct ggaagatttc tatagaacta aggatattaa
    32641 agatagaatt aatcaacgtt catctgattt aaagaagagt atatctgtta agcttgatag
    32701 actttataat aaacttaaaa aacaagaaga agaattatca gaatccgaaa atgcagacat
    32761 ttataagata aaaggagaat tgattacttc atatatttat atggtagaaa aaggtatgga
    32821 aagtattgaa gttgctaact tctatgatga aaattgtaat gatgtaacaa tagaattaaa
    32881 taaaaacttg acaccatctg aaaatgctca aaaatacttt aaaaagtata acaaaatgaa
    32941 acatgctaaa gtcgagatat ctcatcaaat ttccttaaat aaggaagaaa tagattactt
    33001 agaaaacata attttaagta ttgaaaactg tgaaaattta gctgaattac aagatataaa
    33061 agaagaactt gctaaggttg gatacatcaa gactcaaaag aaaaacagta aaaaggatac
    33121 tatcccatct acaaaacctc atgaatttgt ttcttctgat ggatttaaaa tattggttgg
    33181 taaaaacaat aagcaaaatg actatcttac actaagactt gctgataatg atgacttatg
    33241 gatgcataca aagaatattc ctggttctca tgtaataata aagtgtgctg gtaaggaagt
    33301 ccctgataat actgtatttg aaggagctat gctagcagca ttctttagta agtcaaaact
    33361 ttcttctcag gtgcctgtag actatactaa gagaaaaaat gtgaaaaaac ctagtggttc
    33421 taaaccaggt atggttattt atgaaactaa cagtactatt tatgttactc ctgaagaaga
    33481 aacagtagcc aagcttaagg ttaaatctga ataaattctg cactaaatac aaaataaatg
    33541 ttagacaact acacattatc attctaaata aaaatagtgg ccttcttata aagtacaata
    33601 ataaggccac tttctaatta gcttactttt aattttctaa caaaatactt ttctgtttta
    33661 ataactatac ttatattccc aacaaatatt ttcagtcttc ttatatagat gtaattaaaa
    33721 agttaacact aaaagctatt ttttaattta ttgatttgtt ttagtaattc attatattct
    33781 gattcataca attctttttt tagattatct ttttctatag ataacataga tataacagtt
    33841 gtaagtctat tttctaatac tagaagattt tcttttttga tttggttttc tctactagga
    33901 gtttttttat ttctgctaat tgtatattcg tcataattac aatcaaactc ccttatttta
    33961 gcatctttta tctctataat atagttacaa atatgtgata caaatactct atcatgagac
    34021 actataagca tagttttatc agtatttatc agtgcctttt ccagtgcttc catagatttt
    34081 atatctaagt aatttgttgg ttcatcaaat actaaaaaat tattatcttc caatattatt
    34141 ttacatagtg ctatttttac cttttctcca ccactaagta cttttacttt tttgtagaca
    34201 tcatcacctt taaacccaaa taggcttaag tttatcctta taaaagattc atcaaatgaa
    34261 ctattaactt tagtattgta tagaacactc ttttcctcat ctaataaact ttgattttgg
    34321 tcaaaataac ctacttttac cttgttattt atttttatat tgtcattttt atcagccaat
    34381 atttctttta tcagagtact ttttccacac ccattatcac ctaataatgc tatcttttta
    34441 cctcttttta tcttaaagga aacattatct aacaggagct tattttctaa ctttagagtc
    34501 atatctttta cctctactag atttttacta attatctcca taccatcttg aatcttaatg
    34561 ttcatttctt tagaaacttt aggtttattc tttacctcaa gtttatcaat tctgcttttt
    34621 atagccttca catttgagtc caactttttc ttattagtct gaccacccat tttgtgcaac
    34681 ctagcttctg aattacccat tctttttggt gtttttctta tgccatcact taaagctttt
    34741 ttatataaaa gagctttttc aagccttttt ttctctttaa catattcttg atactcaaaa
    34801 tcagctcttt ttacttcttc atctttttgc attaaataat cagtgtaatt gcctttgtaa
    34861 gcttttagtt ttccatcttc tagttctaat atagttgtac ataactcatc cagaaaacgt
    34921 ctatcatgag atattaataa taatgctccc tcatgccttt taaacatatc ttccaaaacc
    34981 ccaatgcttt ttttatctaa gtttgaagtt ggctcatcag ctattatgat atttttctta
    35041 ttactcagcg catctgctat ttttaactta accttttccc ctcctgataa gtgttcttca
    35101 aatttatctg gagcatttaa aagactcttc tccttactat gactacatgt ttcaatatta
    35161 ttttcactct gacttatata agaatagctt tctgtaaggt aagtatatcc ttcatcaatt
    35221 tctatttctc ccaatattgc ttttaataat gttgtttttc cttgaccatt tgaaccaact
    35281 agacctattt tgtcattttc taatatttca agtttatcta tatccaaaat taatttatct
    35341 gcataatatt tctttaaatt ctctactttt actaacaaca taaaaaatcc ctccttaaca
    35401 cacttaaata taaggagaga ttctataaat aggcataaca aataaacgta tagactataa
    35461 tctactctcc ttaatttaag cattgcaaat aaagccacca aaataggctt ttcaaaaatt
    35521 acaatacttt aaattataaa aaatagatta tattctcatt gtcctagtta ccttgccctt
    35581 tctttcattt atttttaatc attatatcaa cattaattac aaatgtctat agaaaattaa
    35641 ctaattttag ttattcaata tacaacttat tctttaaatt tattcttctt caactttaaa
    35701 gtcctgatta tttaaaattc tgtttaatac aattccaaca attgcagcta aactaagacc
    35761 agttatcttt actgtacttg ttatagggat tccaacagaa atacctttat tagataaata
    35821 tgttgttcca agacctatta ataatataac tgctatgatt aatatattta ttttactttc
    35881 tacacaagaa ctgtccctta tagtttttaa tcctacataa gttatcatcg aaaatagcat
    35941 tatactaatt ccacccataa ctgaacctgg tatactttga agaaatccac caaacttgcc
    36001 tacacatgca agcaatatag caaatattgc tgtacgtcta agtattgaag ggtcatagtt
    36061 tttagttata gcaagtactg ctgtattttc tccataagtt gtatttgctg gtcctcctaa
    36121 acaccctgca actatagtcg caaatccatc cccaagtaat gttctattta atccaggatt
    36181 ttctataaag tttttaccta ctacagcacc attagtagtc atatctccta tatgttccat
    36241 aaatacagct aatacaactg gagatataat cactattgct cctaaactga acttaggaac
    36301 tgtaaagtta ggtattgcaa ataaacttgc ttctgtaata gtagttatat ctactaatct
    36361 taatatcaaa cagattatat acccagaaaa aactgctatt aatattccta attgttttat
    36421 aaatcctcta ccaaacttat tggttaaaat tgctattgct aaagttataa atgctatcac
    36481 aaaattggct gaagccatac taaaagctgt tggcaataaa tttaatccta taactactat
    36541 catagctcct gtaacttgtg caggaaaata taatttaatt ttatttacac caactacttt
    36601 tactataatc gacattataa tataaattaa tcctgctact attataccac cttgagcata
    36661 tgctaaatct ccaccatacg cctctttagc tgctagtata actggtataa acgcaaaact
    36721 tgaacctaaa aaggcaggta cttttccttc tgtacaaaaa tgaaatatca atgtaccaac
    36781 accagcacag aaaattgcaa ctgacggatt aaaacctgtc aaaatgggaa ctaatactgt
    36841 cgctccaaac atggctaata gatgttgtaa agacattatc gtttttttca taaaaacctc
    36901 caaaattatt atatataaat aaatcctctg tttaattaat accttttaat taattatcat
    36961 tccctagatt ctatttttta acctctatat acttattata taatttatca ttattttaca
    37021 ttttacgaca tcaaaaactc aaataaagga gaataaaaac tacattaaga ctcacatcca
    37081 taaaattata atatttattt tgtgcaatct atgtaagctt tatcctcctc cctgactatt
    37141 caatgctaag cttttatata atacttaaaa ataattattc tttgattgta actgaatctt
    37201 ctccatcaat ttctaataaa ctgacagata taatttcatg tctagaagta ggtacatttt
    37261 ttcctacata atctgctctt ataggcaact ctctatgccc tctgtccact agcactgcta
    37321 attgtattga tttaggtctt cctatatcta taatagcatc tagtgaagct ctcacagttc
    37381 ttccagtgta taaaacatca tcaactaaaa ctactgtctt atcatttaca ttaaaatcta
    37441 aataagttcc tttaactaca ggttcaacat gaattttttc caaatcatct ctatacaaag
    37501 ttatatcaat atctcctgta tctacttttg ttccttctat ttgttctatt ttcttagaaa
    37561 ttctatttgc aattggcaca cctctagtct ttattccaac taaaactaga ttttctacac
    37621 ctttatttct ttcaattatt tcatgactta ttctagtaat tgctcttgct attgcctttt
    37681 catccattaa ttgagctttt tctaccatat cataattatt gtcaaagata taatatatct
    37741 atttgacaaa tctactcctt tcttttaatt ttttataaat ttatttttat ttaaaatttc
    37801 aatttaaaca acaaaaaaac ttctcatcac aagacaagaa gctcttatat aaaaaatata
    37861 cagtgtaatt tagcaattgt cttacgatat ctctgtatcg tttaaagata attttttaat
    37921 ctatcaactc aaattatata tttatatttc aaatttttca agctttttta aacaaaatac
    37981 tttaatataa gatattttat ttaaatttca ctaaatttat ttacttatat aaaaacaaaa
    38041 aatgatttat aagtattata caaatggtta ttttatcttt ttaataactt ccttaaaata
    38101 ctcaggtaat tctgaattaa attcgatata ttccttagta gtaggatgta taaatcccaa
    38161 cttcttagcg tgcaaagttt gtccttttaa tttaaattta gtttcttttg gaccatatac
    38221 ataatcacct aaaagtgggt gatttataga caaagcatga actcttattt gatgagttct
    38281 accagtttca agcctagctc tcatatgtgt aaacttatca aatctttcta caacttcaaa
    38341 gtgagtgatt gcattttttc catcttttac tacagccatt tttaatctat ccttaggatt
    38401 tctccctata ggtttatcaa cagttatttt atcttcttta accacaccat agcatataaa
    38461 ttcatactcc ctagttatag tatgttcctt taactgttct gctagaaaat tatgtgcata
    38521 attgttctta gctatcataa gaagaccaga agtatctttg tctattctat gaactatacc
    38581 aggtctaata actccattta ttgatgacaa cttatcttta caatgatata aaatcccatt
    38641 tactaacgta ttttcatagt ttccaggagc aggatggaca accatatcct gaggtttatt
    38701 tattattaag acatcatcat cttcatatac aatatctatt ggaatatctt gaggtaaaac
    38761 ttctaatact ttaggttctg gtatctctat aacaatatta tcttcctctt taaccagata
    38821 tctagctttt tctactttat tattaacctt tacttttcca tcttttataa ttttttgtat
    38881 ataacttctt gacatatcac ctaattgttc tgctagataa atatcaagtc taacttcttc
    38941 ttcctcttca agtactaaaa actcttttac ttcatccatt atatcaccta cttttacttt
    39001 caaaaaataa aacatatata cataaaaaca cagttcctac aactacaaat acatcagcta
    39061 cattgaatac atattcccat ataactctaa agtcgaagta atctaccaca aaacctaatc
    39121 gtactctatc aactagattt cccaatgcac cagatataat taatataatt cccaaccttc
    39181 caaatatatg tactttcctt gtattaagat agtataatcc aaataccgtt gcaagtaatg
    39241 caacaattat aaatatccat tgattatttt gtaataatcc aaatgctgca cctctatttt
    39301 cgacataagt taaatgaaat acattattta ttattggtat tgttgataca tccaccaaat
    39361 tattcaatac ccatatttta gacagttggt ctaaacctat gagtagaatt attattaata
    39421 tatatagcaa tgatttttcc ccctataaat tatttaaaaa ccaattttat ttgaagcaca
    39481 ctattattat tatacaaaaa aaatgctcta aagagcaact ttattaaaat tgtattttct
    39541 taataaagct agaacaaaat ttaaaatagt actacttttt ttatattgta gatttctaca
    39601 tatttaaaat catatcatta tgtataaaaa atttaaaaat accatactaa tcaatatttt
    39661 ttgtgtattt tatagattaa agattaatga tgtctgtaat ttaaacttac aatcaccact
    39721 aatcttcaaa caaatatttt tacttttgca cttgcttatt tttatctaaa tgttgtataa
    39781 gttcagatac tgttacgaat ttatatcctc tctcttttaa tttaggaatt actacttcta
    39841 aagcttgtat agtttgtgat ttatcatttc ttttagtgtt ataatcatgt aaaagtatta
    39901 tattaccatt ttggaccttt gtaaggatag tatttataat tgcatctact ccagggttag
    39961 accaatctct agggtctaag tttgaccaaa gaattatatt cattccttta gaaactataa
    40021 tatcacagac acttttactc atggctctat atggaggtct aaatatcttt ggttctactc
    40081 ctatcacact ttttataata ttttgcgtat ctgttatttc tttttcaaca ctactataac
    40141 tattttttgc aacatttata tgtgtaaatg tatgattccc tatttcatgc ccctcatcat
    40201 attgtctttt taataattct ttgttatatt ctactttttc tccaactaca aaaaaagttg
    40261 cttttacatc atttttcttt aatatatcaa gtacttgtgg tgtaaaatcc tcatctggac
    40321 catcatcaaa agtcaatgct aaaatttttt catcctcatt tccttttttt attattacat
    40381 cttcataatc gtcttttata ttgtcattta aactatatgc ttcttttgaa ctatcttgat
    40441 tatgtgtatt atgattatat gtatatacac ctactataga taataaacat atacatatac
    40501 cacatattat tttcttcatg tcattacctc ttttctaatt cattctagca atatatttta
    40561 taatgtcagt tttttaatct ctcaatattc catttttatt taaatcagtt aaatttgtta
    40621 ggtcttctga ataaaagtta gaatcttcta catcagggtt ttgcattgca tcacctaata
    40681 aattattttc cttctttgca caatcattac ataaacttgt atctggcaat atatccaatc
    40741 tttctaaatc tatttgatta tgacataaat cacaaatccc atatgttcca ttctctattt
    40801 tagataatgc taagtctatt tgatacaatc ttccctcttc atgatttatt agactgtttt
    40861 gcatattttg catatataaa tctgttccca tgtctcctat atgattatca taactagata
    40921 attctcctga tgaatacttt tcacttgtat gtttagttgt atctccaaat aaagtattat
    40981 ctttcatatc ctctactaaa ttagttaaat tttctctttc tttcaataat ttatctttat
    41041 attttttagt atccattttt aacacctcta attttaatat ttacatcata aaaatagtat
    41101 gtggaaaagt aaattaaaca tacaataaaa ataagatttg attcgaaaaa tggaatgtct
    41161 caaaatgaaa tttttagttc atttaaccct cttttttata tgaaatcaaa tatattttta
    41221 tcctttcagc aataaaaaag agacttatct ctattttgag atagtctctt ctttgaacat
    41281 aatttaaata caatttttta atttttatag ttattatttt actaacttgc caatttatga
    41341 ttattttgtt ctttaatatg ttttgctttt ttaaagaata aaagaccaag aattattgct
    41401 acaataacac ctattccatt agatatatta actggtagtc taaatccttc ttttgcttgc
    41461 attatataac taaatgttac tacagtcata aaaagtgctg gtaatgtagt tatataatgg
    41521 tttttatttg ctttgactaa atatgcagaa ccagtccaca acattatcat tgcaagtgtt
    41581 tgatttgccc aagagaaata tctccaaatt atattaaaat ctataaatgt aagagcaaca
    41641 ccaactgcga ataatggtaa agctattaaa aatctatttt tgactgcatc ttgattataa
    41701 ttcattgcat ctgctattgt cagcctagca cttctaaatg ctgtatctcc agaagttata
    41761 ggacatgcca caactccaag tatagctaaa gctcctccaa taggccccat taatccaaca
    41821 gatactgtat ttactactgt tgctgctcca ccatcattaa atatcacatt aagttgaggt
    41881 atgccatgga aatatgacat ggctgctgct gcccaaacta aagctattat accttctgca
    41941 accattgacc cataaaatac tggtcttgcc tcactctctt tctcaacaca tctcgccata
    42001 agtggtgatt gagttgcatg aaaacctgat attgctccac atgctattgt tataaataag
    42061 tatgggaata aaggtgttcc atttggattc atattttgta aatttatatt tggtatgtta
    42121 taccccttta ttattaatcc tccagctaca cctactgcca taattaataa tgctgctcca
    42181 aataaaggat atattttacc aatcaattta tctattggta aaacagtagc cataatataa
    42241 taaagtatta tcactccaac ccatatttgt gcgtttacac ctgtcaatgt ctttaataat
    42301 cctgctggac ctgttacaaa tacaactcca accaatacta aaagaataac actaaatatt
    42361 ctcattgcta tttttgcacc atttcctaga taaaggccta ccaactctga tgcagatgta
    42421 cctttgtttt tcattgatag atatcctgaa gcaaaatcgt gtactccacc agcaaagatt
    42481 gttccaaaca caatccataa aaatgctgat ggtccaaata atgctccttg tatagctcca
    42541 aatatcggac ctaaccctgc tatatttaga aactgaatta aaaagattct cttccatttc
    42601 attggcacat aatctactcc atccctacaa gctatagctg gagtttgatt tttgtcattg
    42661 gttccaaaaa ccttttctac aaaagtacca tagacaaaat aacctactat aagtactact
    42721 attgaaccta aaaagcttac cataaattca tccccttagt ttttatataa ttgctttata
    42781 aatttttatg agtttaagaa aactatttcc agttcaaata gtaatacttt tttatgctaa
    42841 tataaatatt aattacatta agtgcatatt taagtacatg aaatgacttt atatatgcat
    42901 taacagccta aattctttta ctttgcttct acttacagtt acttcataat cactattttt
    42961 taattttaat atgtatgtat tattaaacca tggtataatt tcttttactt tggttaagtt
    43021 tacgatataa gacctatgtg ttctaaaaaa tgttttatca tttataagat tttcgacttc
    43081 ttatatgcct tcttttaata taaacttttc tttctcagta tatataaatg tctgcctttc
    43141 attagcttca cagtaatata tatcatcaat atctattaca actaatttat cacctttcca
    43201 aaggctaatt ttatgtgtta tttcttctat ctcctgattt actgcttcct tcttatattt
    43261 gtataaatta ctatttacat taaatagttc aatatcactc atctcacttt tttctaattt
    43321 attcagcata gaaataattc tctcttcaga atatggtttc aatatataat caaatacatc
    43381 caaagaaaaa gcatctactg cataactttc atatgctgtt ataaaaacaa ttttaggtct
    43441 tgatttaaac tgatttaatg tttttgcaag tagcattcca tcaagatgag gtatatttat
    43501 atctaaaaaa attacatcta ttttattttc ctgaataaag tctaacacct ctataccatt
    43561 ggtaaattca cttactacct cgattccact tttattttca ataaaatatc tcaattcctt
    43621 tcttgctgga aattcatctt ccactattat agctctcata cttctactcc tttacataaa
    43681 atactatcaa agtcccatta tctattcttc ttatattaag ccctcttcca tacatcagtt
    43741 taagccttaa atgtacatta taaagcccaa tcttattttc tggcatatta tcttgatata
    43801 cattatttat aatttcctca cttataccta taccattatc ctctattgat attctcacag
    43861 tattttctaa tgaatacggt aactttttta tggatatttt cactacacca gctctaccac
    43921 tttctaaaat accatgtatt attgcatttt ccactaatgg ttgtataatc aaacttggaa
    43981 tctttatatt tacatcttct atatcataag atacagttag taattctcca aacctagctt
    44041 tttctatctc tacaaaagat ttaacttgtt caatctcttt atttatatca attaagttat
    44101 cactatattc aagattatat cttagataag ttgataaatt tattattaac tctctagcct
    44161 tatctggact aaatcttata aatgaagcta ttgtgtttag ggaattaaac aaaaaatgtg
    44221 gattaatttg tctttgaagt gcttttattt ctgctttatt tctcgcttct atatcttttt
    44281 tatctttctc aatcccctct actatagata taacaaatcc tattccaatc tgcccaacta
    44341 ttaaaggtaa atatattcca ccgattattt gaattgctaa agaatgtgga tatgatatta
    44401 agtatataag taatatagta aaactttcag aaatcatgcc taccaatatt ccatacatta
    44461 ctctatgttg ttttggtatc ctcttatata gaaatcctga taatacacct gctagtatac
    44521 ttgataaaag acatggaaca gatgttatcc ccccaatatc cataaaatat ctatgaatcc
    44581 ctgaaaaaac tcctgctgta atacctacca tcggaccaaa taaaatgcca cctgatacta
    44641 tagatattat tctagtgttt actattgacc ccatataatt tataccacta taagttccaa
    44701 atatggctaa aaaagtaaaa actaatgcta tacagaccaa gtcattaaaa ctatattctt
    44761 ccttttgaaa tatctgctta aatattttta actttgttat tagaagaaac aatactacaa
    44821 taaggcttgt cttttctatt aatgcgatta aaattgatgt tttcataatc cccctttaaa
    44881 aaataaattt ttacacactc taaatttatt atggttgtta taaattacat atttgaagta
    44941 atcctatcac aaaactttcc acatttaatc ttttaattaa ataagaaata gttgtacttc
    45001 tattaacaac taatactagc tagcataagc ttgtaataac ttgtagttat tattaacttt
    45061 acaattctag tatttaattt tatactttag aaatctcttt tcttagaaat aatactttcg
    45121 aatatcatat aatacaatat tatgtagatt cttactcata caacatattt ctttaaaaat
    45181 tttatactat tctttgttca aatatctcat ttgatttaat tgtgttaaac acacattatc
    45241 atctctctgt tatatattta ttatatagcc gtatatcgat aaagtaaata aatcccatat
    45301 ttaaaataat atgtttgaca ataaattatt ataatgtata tttaccataa taatttgtca
    45361 accttgaaag ttacttctat aattattgtt aaataattct tctaaaaaaa atacctataa
    45421 attttatttt ctgaaataca tagttataac tatt
//
LOCUS       DAYAOX010000006        44458 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.006, whole genome shotgun sequence.
ACCESSION   DAYAOX010000006 DAYAOX010000000
VERSION     DAYAOX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.006"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(123..728)
                     /locus_tag="ACGI57_000271"
     CDS             complement(123..728)
                     /locus_tag="ACGI57_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFG9715361.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(853..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1030..2196)
                     /locus_tag="ACGI57_000272"
     CDS             complement(1030..2196)
                     /locus_tag="ACGI57_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFG9715362.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(2344..4485)
                     /locus_tag="ACGI57_000273"
     CDS             complement(2344..4485)
                     /locus_tag="ACGI57_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFG9715363.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(4629..5736)
                     /gene="prfB"
                     /locus_tag="ACGI57_000274"
     CDS             complement(join(4629..5660,5662..5736))
                     /gene="prfB"
                     /locus_tag="ACGI57_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFG9715364.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(5751..8426)
                     /gene="secA"
                     /locus_tag="ACGI57_000275"
     CDS             complement(5751..8426)
                     /gene="secA"
                     /locus_tag="ACGI57_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFG9715365.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(8558..9109)
                     /gene="raiA"
                     /locus_tag="ACGI57_000276"
     CDS             complement(8558..9109)
                     /gene="raiA"
                     /locus_tag="ACGI57_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFG9715366.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQENYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(9662..10336)
                     /locus_tag="ACGI57_000277"
     CDS             complement(9662..10336)
                     /locus_tag="ACGI57_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFG9715367.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(10339..11391)
                     /locus_tag="ACGI57_000278"
     CDS             complement(10339..11391)
                     /locus_tag="ACGI57_000278"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HFG9715368.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(11440..12399)
                     /locus_tag="ACGI57_000279"
     CDS             complement(11440..12399)
                     /locus_tag="ACGI57_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HFG9715369.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(12492..13013)
                     /locus_tag="ACGI57_000280"
     CDS             complement(12492..13013)
                     /locus_tag="ACGI57_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdB-related putative oxidoreductase"
                     /protein_id="HFG9715370.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            complement(13024..14358)
                     /gene="celB"
                     /locus_tag="ACGI57_000281"
     CDS             complement(13024..14358)
                     /gene="celB"
                     /locus_tag="ACGI57_000281"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFG9715371.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(14397..14723)
                     /locus_tag="ACGI57_000282"
     CDS             complement(14397..14723)
                     /locus_tag="ACGI57_000282"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9715372.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(14747..15055)
                     /locus_tag="ACGI57_000283"
     CDS             complement(14747..15055)
                     /locus_tag="ACGI57_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9715373.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(15196..17109)
                     /locus_tag="ACGI57_000284"
     CDS             complement(15196..17109)
                     /locus_tag="ACGI57_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9715374.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(17441..18223)
                     /locus_tag="ACGI57_000285"
     CDS             complement(17441..18223)
                     /locus_tag="ACGI57_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFG9715375.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(18226..20445)
                     /locus_tag="ACGI57_000286"
     CDS             complement(18226..20445)
                     /locus_tag="ACGI57_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFG9715376.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEENFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(20709..21806)
                     /locus_tag="ACGI57_000287"
     CDS             complement(20709..21806)
                     /locus_tag="ACGI57_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715377.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(22121..23314)
                     /gene="metK"
                     /locus_tag="ACGI57_000288"
     CDS             complement(22121..23314)
                     /gene="metK"
                     /locus_tag="ACGI57_000288"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFG9715378.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(23451..23554)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            23743..24252
                     /gene="yyaC"
                     /locus_tag="ACGI57_000289"
     CDS             23743..24252
                     /gene="yyaC"
                     /locus_tag="ACGI57_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HFG9715379.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(24363..24791)
                     /gene="fabZ"
                     /locus_tag="ACGI57_000290"
     CDS             complement(24363..24791)
                     /gene="fabZ"
                     /locus_tag="ACGI57_000290"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFG9715380.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNITVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(24807..25826)
                     /locus_tag="ACGI57_000291"
     CDS             complement(24807..25826)
                     /locus_tag="ACGI57_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HFG9715381.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(26023..26283)
                     /gene="spoIIID"
                     /locus_tag="ACGI57_000292"
     CDS             complement(26023..26283)
                     /gene="spoIIID"
                     /locus_tag="ACGI57_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HFG9715382.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(26381..27049)
                     /locus_tag="ACGI57_000293"
     CDS             complement(26381..27049)
                     /locus_tag="ACGI57_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFG9715383.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(27115..28179)
                     /gene="spoIID"
                     /locus_tag="ACGI57_000294"
     CDS             complement(27115..28179)
                     /gene="spoIID"
                     /locus_tag="ACGI57_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HFG9715384.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(28266..29519)
                     /gene="murA"
                     /locus_tag="ACGI57_000295"
     CDS             complement(28266..29519)
                     /gene="murA"
                     /locus_tag="ACGI57_000295"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFG9715385.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(29540..30277)
                     /locus_tag="ACGI57_000296"
     CDS             complement(29540..30277)
                     /locus_tag="ACGI57_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HFG9715386.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(30733..32028)
                     /locus_tag="ACGI57_000297"
     CDS             complement(30733..32028)
                     /locus_tag="ACGI57_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFG9715387.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWREESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(32479..34311)
                     /gene="glmS"
                     /locus_tag="ACGI57_000298"
     CDS             complement(32479..34311)
                     /gene="glmS"
                     /locus_tag="ACGI57_000298"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFG9715388.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(34578..35924)
                     /gene="glmM"
                     /locus_tag="ACGI57_000299"
     CDS             complement(34578..35924)
                     /gene="glmM"
                     /locus_tag="ACGI57_000299"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFG9715389.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(36094..36651)
                     /locus_tag="ACGI57_000300"
     CDS             complement(36094..36651)
                     /locus_tag="ACGI57_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFG9715390.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(36652..37404)
                     /locus_tag="ACGI57_000301"
     CDS             complement(36652..37404)
                     /locus_tag="ACGI57_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFG9715391.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(37404..38483)
                     /locus_tag="ACGI57_000302"
     CDS             complement(37404..38483)
                     /locus_tag="ACGI57_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFG9715392.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(38512..38727)
                     /locus_tag="ACGI57_000303"
     CDS             complement(38512..38727)
                     /locus_tag="ACGI57_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9715393.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(38770..39447)
                     /locus_tag="ACGI57_000304"
     CDS             complement(38770..39447)
                     /locus_tag="ACGI57_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9715394.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(39437..40516)
                     /gene="buk"
                     /locus_tag="ACGI57_000305"
     CDS             complement(39437..40516)
                     /gene="buk"
                     /locus_tag="ACGI57_000305"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFG9715395.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLA
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(40538..41440)
                     /gene="ptb"
                     /locus_tag="ACGI57_000306"
     CDS             complement(40538..41440)
                     /gene="ptb"
                     /locus_tag="ACGI57_000306"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050182 - phosphate butyryltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HFG9715396.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(41744..42925)
                     /locus_tag="ACGI57_000307"
     CDS             complement(41744..42925)
                     /locus_tag="ACGI57_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFG9715397.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESMEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(42922..43761)
                     /gene="cdaA"
                     /locus_tag="ACGI57_000308"
     CDS             complement(42922..43761)
                     /gene="cdaA"
                     /locus_tag="ACGI57_000308"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFG9715398.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
BASE COUNT        13247 a         8461 c         4733 g        18017 t
ORIGIN      
        1 atacattttt tgataaatta ataaagccat aatatattta atatatcaat cacaatatag
       61 gactgaagtt ataaataaat tatggctatt caatacattt aattttatat tttcttgtct
      121 aattatttct ttattatatt tcccattttt ttataaagtg gcaatgttac taaagatatt
      181 ataacagcct tcagcacatt aaatggagct atcatccaaa ttacaaaagt gaataaatca
      241 tgaacttgag gatttatagc tgaacctaaa cctattatag catctagtcc cataagttga
      301 ccataaagtg gcaacattac aaaataattt actaatgaac caattatagt catagatatt
      361 gttcctaata caaaaccaat cagtacacct tttaaatctc cacttctctt atatgaataa
      421 caaagtatca ttacataaga accaccaatt aatatatttg caaattctcc tattcctcca
      481 gttgttgttg ctgttattgc ttgtaacaca ttttttacta caactattcc aaatccaact
      541 aatggtccta gagacattcc tccgaatatt gctggaatat ctgataagtc aatttttaag
      601 aaatctggaa atatacctgg tattggtgct tgaataaaca ttaataaata agatattgca
      661 gataaaattg acattactac taaagttctt gttgaaatca tattgcctct ttttgctgtg
      721 ttttgcataa ttattttccc cctaatcaat atgtttatat tttttaggaa aaacaaaaaa
      781 ctcgaagacc ctatcttcga gtttttatgg atatagttta aacaaactaa aataaactta
      841 tttatcttcc ataaatatct tctctcatcc agactttact gtcggctttg gaatctcacc
      901 aaatcaacca cctaagtagt tcgcgggcta taccgccggt agggaatttc accctgcccc
      961 gaagatcttc tttgattttt taattttcct tacactctta tattagttaa ttttttaata
     1021 attgtcaagt tattttttct catacactac tttgccattt attatagtgt aaagtacatt
     1081 actttgaatc tctagtggag aattatccca aattactagg tctgcatctt taccaacttc
     1141 tatagagcct actctatctt ctattcctaa agccttagct ggatttatcg taattgattc
     1201 tattgctttt tcttctttca taccatgttt tactgcaata cctgcacata ttggaagata
     1261 ttgaactggt attactggat ggtctgtcat tatacataca tcaagacctg catttgaaag
     1321 tattcctgct gtattaaatg tcaaatttct aagctcaaat ttagaccttt ctgacaaaga
     1381 tggccctact attacaggaa acccttcttc cactaattca tctacaataa gatgaccttc
     1441 tgtacaatgg tctaatgtaa gctttaaatt aaattctttt gcaattctta ttgctgtaaa
     1501 catatcatct gctctatgag catgtgcctt aaaaggaatt tctcttctta aaactggtat
     1561 taaactttcc atttttatat catattcagg tttttcacag tcttcatcat catgactctc
     1621 atacatatca atatcctcta aatattcttc tgcttttttt aggttttctc taagtaaagc
     1681 agctatagcc attctagttt gaggagattt atcatcctga ccataacagc tctttggatt
     1741 ttctccaaaa gcaattttag atgcaactgg attttttatt accattttat caattctttt
     1801 tccacatgtc tttatagcta tacattgacc acccataaca tttgcacttc caggggttgt
     1861 acaaacagaa gttattccac cttcaaaagc ttctttaaaa gttctatcca ttggattaat
     1921 tccatctata ggatttaatt gtggtgttat tgggtcagtc tcttcatttc catctgctcc
     1981 ttcaaaccct ataccatctt cccataaacc taagtgagta tgagcatcta taaatccagg
     2041 aaacacaagt ttattactag catctattac ctctacatct aaaggagcta tcaaatcttc
     2101 tccaatttct attatttttc cttcatctat aagtatatct ccatgaatta tcccatttgt
     2161 tatggtattt attttaccat tttttataaa aatcattcat tttcctcctg attttcaaga
     2221 ctatcgttct tattttaata tattttctta ctatatactt ttacaaattt ttaacattta
     2281 tacactcgag taatcttatt tacaaatata gaatctaagt actataacca tacataaact
     2341 tatctatttt ttcattgata aggcaactct ttttttgttt aagtcaattc caatgacttt
     2401 tacatctact atatcaccta ctgtaacaat actcataggg tctttcacaa aactattact
     2461 catttctgat ttatgaacta atccgtcatt ttttattcca atatctacaa atgcaccaaa
     2521 gtctacaaca tttcttattg taccttttaa agtcataccc tcttgtatat cttcaatttt
     2581 taagacatca gttctaagta taggttttat accttcttct cttgggtctc ttcctggttt
     2641 tttgatttca gctatgatat cttttaacgt aacttgtcct acttccagtt tttcacttaa
     2701 ttccctcaag ccaatttctt ttattttttc atctatttca cctatattct tattctttac
     2761 atcatctaaa gaatatccaa ttatctcaat cattttttta catatatcat aactttcagg
     2821 atgcacacct gtattatcta gtggattttt accctctaag attctcataa atccagcaca
     2881 ttgagtaaat gcttgaggtc ctagtctttt tacttttttc aattgtgctc ttgacgtaaa
     2941 atctccattt tcttctctat atgctattat attttttgct atagctttac ttattcctgc
     3001 aacatgttct agtagggagt atgatgcagt atttaaatct actccaacac tatttacaga
     3061 atcttcaaca actccatcta gtacttcttc taatctcttt ttgtttaaat catgctgata
     3121 ttgaccaaca ccaatacttt tagggtctat ttttacaagc tctgctaatg ggtcttgtaa
     3181 tcttctagct atagatatag ctcctcttat tgatacattt atatctggat gttcctcatt
     3241 tgcaagttca gatgctgaat aaacagatgc tcctgcttca ctaactatca catactgcac
     3301 ttcgctatct atttctttta tcatttctga tacaaaagtt tctgattctc tagatgcagt
     3361 accatttcca attgaaatta tatctatatc gtatttttca atcaatcctt ttaaaacttt
     3421 ctttgctccc tctacatcat tttgagggtc tgttggatat actgttgtat aatctagtag
     3481 tttaccattt ttatctacta cagctatttt acatccagtt ctaaatgcag ggtcaaatcc
     3541 cataactact ttgtctttaa ctggtggttg aagcaataaa cttttaacat tctttccaaa
     3601 tacacttata gctctttctt gagctatttc tgttaaatga tttcttattt ctctttctat
     3661 tgatgggaaa attaatcttt tataagaatc ttctattgaa cttacgattt cttctttatt
     3721 tttgaaattt ttatcattta catattcgtt tattatgtaa tttaaaactt tatcattatt
     3781 tatttccaac ttaaccttta ggaagctttc tttttctcct ctgtttattg ccaaaactct
     3841 atgtggagcc atacttttta ctgcttcgct ataatcataa tacatatcat aaaccgattt
     3901 ctcatcagta gcacttttgc taactatcat accttctctt aaagctaatt ctcttatata
     3961 ctttctaatc ttagcatcat ctgagactag ttctgcaatt atatctctag caccttttag
     4021 agcatcttca atagaaagaa cttctttctc ttcatcaaga taattctttg cctctatttc
     4081 tatattatct aaattattct ctagtattga caatgctaaa ttttctaatc ctttttcttt
     4141 tgctattgtt gctctagttc ttttcttttg tttatatgga gcatatatat cttctacttc
     4201 ttgtaatgtt tttgcttttt ctatattttg tgttatttca tctgttaatt tcccttgttc
     4261 atctattagt ctaactacat catcttttct actttgaaga tttctcaaat acattaattt
     4321 ttcataaaat tctcttagtg taacatcact catttcgcca gtcatttctt ttctatatct
     4381 agctataaat ggtattgtat ttccttcatc aataagtttt aatgtattat ttatttgttc
     4441 atctctaaga ttaaactctt tttttaagat ttgatttata tccaatttat tcactcctaa
     4501 tgttagtttt tcataagtat aacaaaacag aacgaaggct gagggttatc gtcctcagcc
     4561 taagtgctgt aagtgcatat taagcactaa ccttcttata catatatctt aattacagtg
     4621 catatttatt aaactatttt attcattttt aagtattcat taataaacaa gtctatattt
     4681 ccattcatta cactatctac atttccaaac tcagcatttg ttctatggtc tttaaccaat
     4741 ttatatggtt gaaaaacata tgacctaatt tgactacccc aagttatttg tgagtatttg
     4801 ccttgtatat cttctatttt ttccttttgc tctaactctt ttaattctat caatttcgcc
     4861 attaaaagtc tcatagctct gtccttattt agatgctgag acctttcatt ttggcactga
     4921 actactacac cagtagggat atgtgttatt ctaactgctg aatcagtcgt atttacatgt
     4981 tgccctccag ctccagaagc tctatatgtg tctatcttta aatctgatgg attaatttct
     5041 acttctatat tttcatctaa ctctggtgtt acatctatag atgcaaatga agtatgtctt
     5101 tttcctgatg ggtcaaatgg agatattctc actaatctat gaactccttt ttcactcttt
     5161 aaatacccat aagcatttgt tccttctaca agaattgtag ctgtctttat ccctgcttca
     5221 gggtctgata ttatatctaa aagcttaact ttataaccct tactttctga ccatcttata
     5281 tacattctca aaagcatttg agcccaatct tgagcatcta agccaccagt accagcattt
     5341 atagataata tagcattatt tttatcatac tctccattta gaagagtctt tattttcatt
     5401 tcagataatt tttcttccat tgattctatg gacttttcta tatctctttc tacagaatca
     5461 tcatcttctt ctaaacctat ttcaattaat acttctatat cttctaataa gctttttaag
     5521 ctttcatatt cttctaatgt ctcttttaaa gatttatttt cttgaagtac tctttgagcg
     5581 acttcattat cattccaaaa atcttgttga ttcatctttt cttcattaat ttgtatattt
     5641 ttcatcaatg aagctatgtc aaagagaaac cctcaattct tctaaatttg aagccatctg
     5701 ctccatacta tgtctatact cttgtaactt taacatttaa tcctccgaga ttatcttcca
     5761 caacagtttt tatatttttt accacttcca caagggcata agtcatttct tccaaccttt
     5821 tcatcttttt taacagtttt acttgttgga agtgttccat ctgatggaga agatagattt
     5881 tcaacatcta caactgcttt tctttccact ggagtttcta ttgttatatt aaataaatat
     5941 cttacagtat cttctttgat atgtttattc atttcatcaa acatatcaaa accttccatt
     6001 ttataagcaa taactgggtc ttgctgacct acagcacgaa gaccaatacc ttggcgtaat
     6061 tggtccatag catctatatg gtctatccaa tggttatcaa cagcttgaag taagattact
     6121 ctctcaactt ctctcattct ctcatagcca acttgttctt cttttgaagt atatatcttc
     6181 atagctattt cataaacttt ttctgttatt tcaactggat taagtttttc tatttctggt
     6241 atttctatgc taccttttgg caagaacaga ttatacatat gttctttgaa tccttcttca
     6301 tcaaatcctt tatcttgtgt ataaagagta actgcttctt ctattataga atgagtcatt
     6361 gattgtattt gttcttgtaa atcttcacct tctaatacac gttttctctc tgcatatatt
     6421 atttctcttt gtttattcat aacatcatca tattgaagta catgctttct tattccaaag
     6481 tttttacctt cgactttctt ttgtgctcct tcaatagact tagataatat tctatgttct
     6541 atcggcatgt cttcttcaag acctatttta tctacaatac cagatattct atcactacca
     6601 aacaatctca taaggtcatc atcaagacct atatagaatc ttgaactccc tgggtcacct
     6661 tgacgtccag cacgacctct caactggtta tctattcttc gagattcgtg tctttctgta
     6721 cctataatag caagtccacc tgcttctaca acttgttttt gttcttcttg tgtttgttgc
     6781 ttaaactttt caaatagttt ttcatactct tctctagctt caaatagaat ttcattacct
     6841 tttacaggta taccttctat tggagtatct actctattta ctatttcttc tttaaatcca
     6901 tttcttctca tttctctctt agttaaaaag tctggatttc cacctagaac tatatctgta
     6961 ccacgaccag ccatattagt agctattgta acagctccaa gtctaccagc ttgtgctatt
     7021 atttctgcct ctttatcatg atgcttagca tttaatacct cgtgctttat gccctttttc
     7081 tttagtattt gtgataataa ttctgatttt tctattgata ctgtacctac aagtattggt
     7141 tgattgactt tgtgcctttc aattatttct tgtgctacag cattaaattt acctatttca
     7201 cttttgtata cacagtctgg taagtcttcc cttatcataa gcttattagt aggtacttgg
     7261 aaaacatcca ttttgtagat tgctttaaac tcttcttctt ctgtttttgc tgtaccagtc
     7321 atcccagaaa gttttttgta cattctaaag taattttgga atgttacagt agctagtgtt
     7381 ttagattctc tttgtatttt aagaccttct ttagcctcta ttgcttgatg taaaccttct
     7441 gaatatcttc taccaaacat aagtctacct gtgaactcat ctactattac tatttctcca
     7501 tctttagcaa cataatcaac atctttcttc attattacat gtgctcttaa agcttgattt
     7561 atatgatggt ataattctgt atgagatata tctgttatat tatcaacatt aaaatacact
     7621 tctgcttttt gtatacccga tgctgttaga gatacagcct tatctttttc ttctaattca
     7681 taatcatctg gttttaaggt taaaacaaat gtgtttgcat ctgaatataa gtgagttgat
     7741 ttgtctccag gtccagatat gataagtggt gttctagcct catctattag tatagagtca
     7801 acctcatcca ctatagcata atttaatcct ctttgaactc tttgctcttt gtgtattacc
     7861 atattgtcct ttagatagtc aaatccatat tcattatttg taccataagt tatatcacaa
     7921 tcatattgtt cttttctaac ttttggattt tgaccgtgta taataacgcc tacactcatt
     7981 cctaaaaact catagatttt tgccatttgg tctctatcac gttttgcaag gtaatcattt
     8041 actgttacaa catgtacacc ttttccagta agcgcattca aataaacagg agcagttgca
     8101 accaaagttt taccttcacc tgttttcatt tctgctattc ttccttgatg tagtactata
     8161 cctccaatca tttgaactct gtagtgtcta agtcctaaca ctctcttaga aacttctcta
     8221 actactgcaa aagcttctgg aagtatatca tctatacttt caccatttgc taatctttct
     8281 ttaaatatat ttgtcatatt tttaagttcc gagtctgcca tagattcaaa tttaggttct
     8341 aatgaatcta ttatatcaac agttttattg aactttttta attctttttt atctgccatg
     8401 ttgaataaat tatccataaa gctcatattt tacatctctc ctcgttcttt attgtactat
     8461 tattcatttt accataaaaa ctatactttt aatattaatt taaaaagcta tcgataattt
     8521 atttatcgat agctaatatg attaaatcta aattgcttta ttcatgttct attataccat
     8581 atccaccatt attacgctta taaactactg ctatttcatc tgaatctatg ttcttgaaca
     8641 tataaaaatc atgtcctatt aattccatct gtaatacagc ttcttcttca ctcattggct
     8701 taacactaaa tttcttacgt ctttgaatta ctaattcttg attttcatcc ccataattct
     8761 cttgttcatc agctagattt atctctgcat cttggaatct tatactcttg ttgtctttat
     8821 gtttatcttg taatctcttt ttatattttt ttaattgttt acttaattta tcatatacaa
     8881 catctatagc tgtgtataaa ttttcctgca tatcttctgc tctaattata ggaccattta
     8941 cagtagctat tgttacttct attttttgtc ttgcttttct cgcacttact gtaactttta
     9001 catcagtgtc tggatgaatg taattatcta acttacttag tttagtttcg attttatttt
     9061 ttattgcatc agttaattcg atttgttttc cagatataat tatgttcata aatacatctc
     9121 ccttcaatta tgccagtata agaaaattta tactttaata atatattcta cacgaactct
     9181 cataatcctc tttttattat ttacttttat gattttttct attcaattga tagaattatt
     9241 tatcattttt agttacattg atacttatgt ggattaatat tattacaaac acctgtggat
     9301 aatgtggata aaactgttaa taactcttgt ttttgtttat ttaaagctat ttttactcct
     9361 atttctatcc acaaaaatct ccattaattg tttattaata tgtgaattca tgtcataaga
     9421 tggtaatttt aggaaattat gtgtattata tgccaatata cctttataat tttttaataa
     9481 atataaaccc tctaaaacct ccataatcta tcttttcttt cattatttat atttaaaact
     9541 aattaaaagt tataaagcat actcaacaat aatttcatat ttaataccta ttaacattca
     9601 tatacccaaa ttataattaa cttactctct ttatttaaac taagtatttt atattcatat
     9661 actatcttga aattatattt acgagttgtt taagcttttg tctatctata tcttcaaaag
     9721 gactgttatt tattctaatc gctgtaccaa aatgaaaatt acttgcctta gttaattcct
     9781 ttatcttttc tatattatta aaattaagac ctccacctaa aagaattttt atgtgattag
     9841 aactaagcat catattcttt atcatttgta tgttatcaac tatacttcct ttccctcctg
     9901 atgttaatat attcgtaatt ttatcataat ccttcaaaat tttaacacta tctattgtat
     9961 ttgattcatc tattgctcta tgaaatgtaa catcaagatt atcacaacat ttcaataaaa
    10021 catttagatt tttctcatct atactattgt ttttatccaa tacaccaaat acaactccat
    10081 tagcaccaat ctctttaacc acacttatat ctttttgcat tatacttata tcttctttac
    10141 tgtatacaaa acttttcgca tgatgtctta tcattacgtt cacaggtatc tttacactgt
    10201 taacaacact ttctataaga ccaaaacttg gtgtcaatcc accttcagtt aaagcactaa
    10261 ctaactctat tctatctgca ccacaatctt ctataatttt agcgtcttct actgacattc
    10321 ctattatttc taacataact actcctttgc taattcatat attgcatttg caaatatctt
    10381 tgcatttaat attatatctt ctatatccat gtattcatca ggattatgag ctattccttt
    10441 ttgtcctgga aatgaaggtc caaacggaac tatatttggc attacttttg catacgtacc
    10501 tccattagtt gttacgggag ttccatctaa tgatgtaacc ctttcataag ctagctgaag
    10561 ctttttaaca agattactct ctttatcaaa atatacagga ttaaaattag aaattacctc
    10621 tatatcgata ctatttggaa aactagcttt tatttcatcc aatacatctt tcaatttata
    10681 actaaaatcg tattttacct ttatatctaa tatagtatca ctattttcta tattcagctc
    10741 tttatatgta acaagcacat tttcagacat attttctata aaacctaggt atacatcttc
    10801 ttcataattg attttacttt ttattctaat ctttgccatg cctttttcac catatacaac
    10861 aggatattta caatctggtg taaatcccat aataggtgct ttttcttttt ctaaataata
    10921 tggtatatct tcaaatccac tttcttcatt tgtaccaaat attattctaa ctctcttatc
    10981 taattttaag tttaattctt taatagcata taatccatat aaggcagaca ttataggtcc
    11041 tttattgtct agtactcctc gtccataaat tcttccatta gtttcttctc ccttataagg
    11101 ctgatgcttc caaccatctc cttcatgtac aacatctaca tgtccaatta cacaaacata
    11161 atcatcacta tctccatgct ctgcataacc aatataatta tccaagtttc taaccttaaa
    11221 tcccaatttt tctgatattt ctaatgcttt attaagtgcc tcccctactt tttcgccaaa
    11281 agggaagcca ttttctgact cccctttgac actaggtatt tttacaatat ctataatatc
    11341 acttaataaa tcttctttta actcatctat tttattactt atgatattca taaaaatctc
    11401 cttattataa aactctattt taattatgta accactactt tactcactta cttctattgg
    11461 tgcttttatt ctagcctcat atgcttccat ccactcattt gatataacct tatgtatagt
    11521 ctttccatct actctatttg aaatatatac agtaggagat tcttctttag ttactactga
    11581 aaaagtaaaa tcagctatat ttgtagctac tccccattca cctttattat tcatggcaac
    11641 taatgataaa tcaccagctt ttcctcttct tttgataagc tgtttatcaa gttcatttac
    11701 tgcaatatca catgcttctt gtggatgctt cccttctttc ataagtctaa ctatctcata
    11761 agatatacat cccttcatta aatcttctcc caaaccagta gctgtagctc ctcctatact
    11821 actatcaaca tagaatcctg aaccagatag tgcagaatct cctattctac ctcttttctt
    11881 cataaacaat ccacttgttg atgtagctgc acacattttt ccagttttgt ctaaagatat
    11941 cataccaaca gtatcatgac cagcatatgg gcttaatcct ttgtctaaag tctctttttt
    12001 tctcttttta taatgtaact tcgctctatc agtcaacata ttttttcttt caaatccatt
    12061 tttatgagca taatcttcag cacctattcc aactaaaaaa ttatttacct tctcataact
    12121 taacttccta gctatgctga caggattaga ataatctctt ataccagcaa ctgcgccaat
    12181 atctaatgtg ttaccatcca taaatgcagc gtcaagttct acctcacaat tttcatttgg
    12241 aagtccacca tatccaaccg atttatagaa tggatagtct tcaactcttc ttacagcaag
    12301 ctctacagca tcacctgctt ccatgccttt ttctaaatgt tctgatgcct cagttattcc
    12361 ttctagggcc attctccatg tcgctatcat tccccacatg ttaaaattcc cctccttaaa
    12421 ttatatagat tttactaatg tatgcttatt ataaatagta taatatttaa acttgctaat
    12481 aattaatact cttattgata acaaaactct tctttcatca aagttatatc ttctttattt
    12541 gctattgctt ttgccaattt acatatgtta tttaatgaat cattaagacc aattccacct
    12601 ttttttggag tcttaactat acatatgtca tttttatatt cgtttatcac atctatattt
    12661 tcttctgaca tagctgcaaa agctggtata tctgttttat catttatatt tttagacagt
    12721 attgctgaca tcttagaaaa gttttcgtaa ttaaagcttg gtccacagat tactacatca
    12781 gggtttaatt tttttaccat agctatcatc ttactcgtta cttcttcttg attatttaca
    12841 aaaaattcat ccccacaaaa taaggtagct attaccttag attcatttaa aaattgctcc
    12901 atcattacac ctggtcctaa tgggcttgat tttccagcag gaggtatatt acttttctct
    12961 tttcctccag ctccagcttg tatttggtcc aatatcataa ctattttctt cataatcttc
    13021 ctcctataat actaaatcat caaaagatat atcatcatct tcctcttcaa cagtttcatt
    13081 ttcttctctc aagtattgct tatctagcat ttttataaat ggtatataca taaatattcc
    13141 tagtataaga agtactaatt gtaagactgc accttgccat cctgttgtta aaaaaccaga
    13201 gaatataatt ggagttgtcc acggtaactg aactccattt gtaagaggca ctaatcctgc
    13261 tgacatacag aagtaagcta ttattatatt tattgttgga actattgcaa atggtatcaa
    13321 tagtattggg ttaagcatta ttggtagacc aaatatcaat ggctcattta taccaaatat
    13381 tcctggtaaa attgatagtt ttcccattga ctttatacgt ttacttttac atattaataa
    13441 catagctatg actaatgata gtgtactacc tgctccacca aatgtcgcaa acatatcttg
    13501 gaattgacct gttattatgt ttggtatttt tgaaccagct tgaaaagcag ctaagttttc
    13561 agctgataga atttttaata ttggattata cacagcccca acaacactac ttccatttat
    13621 tccaaagaac cagaataaat gtaagaatat ataagcgatt gccatagcac ctaaactatt
    13681 tcctaatttt aataatggca tttgtaaaaa cttaaatata aattcatgta tacttccata
    13741 tggtgtagct tcaaaagcta tcttaactaa aaagaaaact accataacta ttgcacttgg
    13801 tattaatgct gcaaatgatt ttgatacagt tggaggaact ccatctggca ttttcaatgt
    13861 ccatcctttt tttacaactg cagcaaataa ttttacagag gttattgctg ttatcatacc
    13921 tacaaacatt cccttagaac ccatccaacc tagtggtata cctgttacca tgtaaactga
    13981 ttctgttcct tctggtgtat atggtattac aaatggtgtt attataaaaa aggcaactat
    14041 tgcaactgct gctgatgcta ttgggtctac atctatttgc tttgcataag aatatccaat
    14101 ccctaaaact gcaagtagag tcattacttc aaatgttgct acagttggtt gttgcaattt
    14161 tacagcccaa tctggaccaa aaatttgacc tacaaattca ttccatcctt ttataggaaa
    14221 atttgctact aataaaaata atgaacctat tattaataat ggtgatgata ctaaaaaacc
    14281 atctctaatt gcaattaata atttattttt acctattttt tcagccaatg gcataagtat
    14341 tctttccaat ctattcatct atccaccctc atttcctatt attttttact ataattctac
    14401 gcttgtttag attttattag ctttatagca agttttaata ctttttcccc attcatagtt
    14461 ccataatcaa cttcattgat tatatctata agttttcctt gagcttcaaa ctttggtttt
    14521 aactccttta gcatgtattt aacttgtggt cctaataata tacaatctgg atttttttct
    14581 tcaactattt tgtcaatttc tgctatcgga aatgcctcta cttctattgg taaattgtgt
    14641 tcatctgcta ccttctgcat tttatctgca agtaaacttg ttgacatacc aaagctacaa
    14701 aataaatata cttttctttt cattttttaa atccccctta atttttttat tttaattttt
    14761 gaatttcttt tcttaacata atcatttctt ttataaggtt tctctcactc atacaagtca
    14821 ttaaatggtc ttgagagtga atcaataaaa tagatatatc tgatttatct cccccagctt
    14881 ccttttgtat aagttcagtt tgtactttat gtgcttttaa aatttcttca tttgcatttt
    14941 caattaattc atctgctttt ttaaaatcgt tttcctttgc atagtcaagt gcttcataaa
    15001 gcatagattt tgcatttcca gcatgaatta taatctttaa aactatttct tccatttttt
    15061 cctcctaagt tgcttctcta cttttagatt atttatacct tacaaacata taatatctta
    15121 taaaaacaaa aaaacatgca caactttttc catcacatgg aaaattttgt gcttatccat
    15181 agttcactat gaattttata taagaatatc ttttaccttt tcaatgtcac atttacttag
    15241 aatatctagc atattttcat cctctgttaa attagatatt tctttaaata attctctaaa
    15301 tacttttcta tcatcatctt ttttatacgc taacaaaagt ataatcttta tatcctttcc
    15361 atttctaatt tttattggtg tttttaacct tgcaaaacat aaaacagatt gttttatata
    15421 tttcatgtct ccatgaggga ataatattcc ttttccaaca ttggttggat atgacttttc
    15481 tcttgttata atagattgta aatactcttt ttctgcataa tgtttttcaa ttaataaatt
    15541 agttaaatat gttatagctt cataaatatc atcatgttct aactctctta ttaaataatc
    15601 agacatcatt aatctgttca taagtttata tgaagtgtct ttttctttta ttttgtttgt
    15661 gaactcttta agtttattct catcctcatc agtaaataat ggtgttgtta taaaaattgg
    15721 tttttggata ttttcatact cttgaaatgc aattataaaa tctatagtct tattaaagtt
    15781 atctcttttt aaatcttcct ctcttaatgt ttcaataatt tttatgttat caaatctctt
    15841 ttctatcttt acttttaata gcatacttac tccaaaacta aatggacata caatacttgc
    15901 ctttttattt gattcccttt tgctgtattt tctttctaga gaggtttgaa agtgaagtgt
    15961 aagataacca atttcatctt ccgttatttg ttttcctttt aattcaattt ctattgaact
    16021 tgatattact gtaaataaaa aacaaaattt agtttttatg ttatctaaaa gtggattttc
    16081 taagtaaaca ttgcttttca tttgatgcat tgtaacattc aaatgacata ttaattgttc
    16141 ataaagtaat atatcattat taaaatttat tcctgattct tctgatacta aatttataat
    16201 tttttttgta taacttgcta attcttcatt tagatataaa gatttcttaa tattattact
    16261 atttatttgt ttgttcatac ccaatattaa agattctata aacaattttt cttcataatt
    16321 taatactatg gataaattag atttcaattc atcttctata tccaatataa gcatttttag
    16381 cttaagctct ttcttagtat ttgatacttt taaagaattt cccagcctta ttcgtattat
    16441 atttataagc aaaaatagca tcaattgctt gaatgatata tctgtaagtc ttatatccat
    16501 atcctcttct atagtaagca ttatgttttt tacaattatt atatctacta tatggaagta
    16561 ctctatttca tctatgttta atctgtattt atctatatat ttaaataaga atgatactaa
    16621 cattattctt atgtctttct catccccttc aatatatata ccactgcctt ttttagtcga
    16681 gatatttaaa ttataattat ttaatgctac tgataattta cttatttctt ctctgacaat
    16741 atttttattt atgtacaact tattagataa atcatttaaa gtaacttttt tattattaca
    16801 aagtagtaaa tttagtatct caacttgctt atgaagctca ctatttactc ttttaacttc
    16861 acctcgcaac ttctgattta ccagtatttt gtctgttcca tctccactaa atttaatccc
    16921 aatattaggt tttcttttga tataagcatt acaaaaagta gataaccatt catcaatata
    16981 tttgcaatca tttcttattg tcctttctga acactcaaaa tcattggcaa tcttattaac
    17041 atttataaac tcttctttta atagcaatgt tgttaataaa ttttcttgtc ttttattcaa
    17101 acatatcacc tctttatgac tcaacctaaa tttattatat ataaaaaaga gctatacata
    17161 aaggtatgct agctctcttt taatatgata tttagctgac ctggtttttg accccacact
    17221 cgcctactgg agggttcgct tgggttcgcc gcactactac tctctctcga ttttattatc
    17281 ggtaggtctt acttctaagc cgcaccatga tcattaaccc aatttttagt gttaacttac
    17341 gtggatccct ttgcctgcga ctggcttgga ctttcaacca cagacctaaa tatcacttat
    17401 tcattttaat attttaaact aaaattgtca atatataaga ttattccata atataattat
    17461 catttgcttt tgtaagtaga gtcaatacaa aaaccttatt agcacctgat aactttaaaa
    17521 gtttagaaat ttcgtttgtt gttagaccag tagtaaatat atcatctatt aaaagtatat
    17581 ttttattatt tattaattta acattttctt taacaacaaa tacatttttt aattcttctt
    17641 ttcgttcttt cttattcaat ttatatagca ttcttgtata tttttttcta cttatgcaat
    17701 ctaataatgg aatatttact attttactta aattaaatgc tattttttgt gcctgattaa
    17761 aacctctttt atttagcctt tttttatgaa gtggaacaaa taatatataa tcaaatttta
    17821 tgttttctaa atataatttt tctttcatta tttgagctat atacttagcc ataaaggttt
    17881 tttggttata cttcagccct aacaccatct tttttgtcac atcattgtat tcaatacaag
    17941 atattgattt atcaaaataa aagtctttat taaaacaata actacactct tcaataaatt
    18001 ctttttcaat tgaatgacgt attattggtt ttccacactt catgcaacca tcttgtataa
    18061 aattcatttc tttaaaacaa cttttacata ttgaataagt atttgttttc tttatggatt
    18121 tatcacaaat tatgcaactt atattctcag gatatataaa atccaaacat ttattaatca
    18181 tatttttaaa attcttaata aaattatcat tgcatatttt catatttatt ctatcaacaa
    18241 cccttcttgt ttaaatttat ttagcttata tcctaaattt gaataccttt gattaactct
    18301 attatttttt atcatatatt caagatattt cacatctcca actaatacaa ctaatttttt
    18361 tgctcttgtt actgctgtat ataataaatt acggctaagt aacataggag gagcccacgc
    18421 tattggcaat actacaactg gaaattcact tccctggctt ttatgtattg ttgtacaaaa
    18481 actatgatca atttcatcta attcatcata taggtatgat acaattttag tctggtcaaa
    18541 taatatataa atagttttct tatctttatc tatgtgataa acatatccta tatctccatt
    18601 atagatacct tctccacttt ctttttggtc ttctgtttcc catttttttg tataattatt
    18661 ttttacttgc attaccttat cgccaactct aaataatctt ttagaaaaat tttcttcaac
    18721 tttaaatttt tcttttttgt taagatactt ctgtaactct atatttagat tagtaactcc
    18781 caattcacct tttctcattg aagataaaac ttgtatatct tttaatttat caactttata
    18841 aaatttaggt aatctttcat taaccaatcc tattatttca tttaaaattt cttcattagt
    18901 tgattttctt ataaaaaaga aatctttacc ttttgtattt agataaagtg gttctccatt
    18961 gtttatctta tgggcattta caattatcat actttcttgc gcttgtctaa atatttcatt
    19021 taatttaact acatttatga tattagaatt aatcatatcc ttaagtacat ttccagcacc
    19081 tacagaaggt aattggtcac tatctccaac taaaattact cttgttccta actttatagc
    19141 tctaagtaaa ttgtacatta agataatgtc taccattgat acctcatcaa caattattac
    19201 atctgaattt attgggtctt cttcatcctt aaaaaaagtt aaatcatcat ctgttgaaaa
    19261 tcccatttct agcaacctat gtatagtttt tgcttcttta ttagaagttt cactcattct
    19321 ctttgctgct cttcccgttg gagctgccag agtcacactt ttaccattat tttcaaatat
    19381 atctatgata gcattaatgg tagttgtttt accagttcct ggtccaccag ttataattaa
    19441 tactccacta ttaacagatt ctttgactgc taaaatttgt ttttctgcta acttaatttt
    19501 cttatcttct tctaaaacat taatctcgtt atctatatct atttttaagt cttcaaattc
    19561 atactgtgat aattttacta tttgactaca tactccattt tcagctaaat agtatggcat
    19621 aaggtatata agattttcat tgtttacttt ctctatatgt attttttgat tataaactag
    19681 catcattaca cactctttta ttatttctcc atttagctcc aataattttt ccgaatcttg
    19741 tattagaaca tgctctggta aatatgtatg cccattactt aaagatttat ttaaagtgta
    19801 taagattccc tgacaaattc tatctttaga gtttttatct ataccaattt tacttgctat
    19861 actatcagct actttaaatc caattcctct tatatcttca gcaagttggt atgggttttt
    19921 atttataact tctattgcac tgcttttata ttttttatat atttttaggc agtaatttgg
    19981 tgttattcca aaaggagata gctgtattat tatatttctc aattctctgt cttcttcata
    20041 actttttact atttgtttta cttttttact ccctatacct tctacttctt gtagtttttc
    20101 tggtgaattt tgtatcacct ctaacgtatc tacaccaaac ttatcaatta ttctttttgc
    20161 cattttttca ccaataccat gtatcattcc agaagataag taaacataaa ttccttctaa
    20221 ggatgatggt gttactggca taaaactatt cacttcaaat tgagtcccat aaatcttatg
    20281 atttacccac tttccttcaa cttctatgct ttctccaatt gctaaagtag gcatacatcc
    20341 tacaaccaca atttcatcat tttcatttac caggtgagct attgtataac cattatcttc
    20401 gtttttaaag actatttcac ttatcattcc ttctaatttt tccattttta tctcctaaaa
    20461 atactaataa tcgctccatt taaatgttag tataatttta tatattaaaa ctatgataat
    20521 cgtagaaaag ttttatattt ttatacttaa cgtaatttcc aactattata cattttatca
    20581 gaaacaaaaa aaatctgcat ccataaaata tggtaccatg aaatacaggt accatattta
    20641 aatagctatt ttattttaat tactctatat ttagagtata aattaaattt cataagtata
    20701 ttacagtact aacttatttc agatttattc tccttgtacc cctcagtttt agtaattctt
    20761 ctataaagca tacatagtaa aattaaaatt cccatgtaac ctgtatatgg atatagtgta
    20821 cctactaact tatcaaatgg cagtaaacct cctatacaag ctaatatact taccactatt
    20881 gttataattt taaacttagg gtggtcatct tctacaaatc ggtttgttac tgaccataac
    20941 aatggtacag ctgtagtgta tattcctagt aaaagtacta ctgagaataa aatacctatt
    21001 attggagata tcttatcagc taagtaaagt gctggtatct cttttgtgta tatatttcct
    21061 atgtctgaaa gtaatgccaa attcatcatt atagctgctg ccataagtgc tactccacct
    21121 actataccac cccatacagc atctttctta tttactgcag aagctcccat tcctgtcaag
    21181 aatgttatta ctattattac attgtaacaa gtataaagta ctcccgagaa atatccattt
    21241 gatgtagcag ttttaatatt taaactatca accatatttc cagcatttga taaagcatca
    21301 atattgctaa ataaactaat tgctcctact aaaagtgtaa atattattat tataggtcct
    21361 atattaccta gtattttaga aagccttgtc aaacctaatg aaacagttat tagggaaacg
    21421 attgccatac caatcctacc tacatatgga tttaatccat agtactctga taatgtagct
    21481 cctgctcctg caatcattat tactaatgtt ccaaataaaa acaatggacc aaaccattca
    21541 aaaaatgttc caagatattt tccacaatat acttgataaa tttttattgg ctccttaagt
    21601 ttcaattctc ttcccttatc tataacttct gctccaaacc atgaaaatag taccatagag
    21661 attattccac ctataatacc cgcatatcca taaaacgtaa aaaattgcag tatttcttga
    21721 ccagttgcaa atccagaacc tattacagta gcaacataag ctccagcaaa acttataacc
    21781 gttttaatat ttactttgcc tgacatacac tactcccccc aattaaaatt atttttaaag
    21841 tgtaagttta ttaagattat attagactca ttattgtgat atttcaatat atttttaatt
    21901 atttaacatt agttaacaca ttcttaacca tcctttaaca tatatcccat aatatgtata
    21961 aatttagtag agtattatat aaaatgtatt gaccagatta aatactgttt attcattata
    22021 tgatgataaa ttaacattta ttaaaaatat ttaactcata ataaaaaagc tgttagaaag
    22081 taatcgtaaa attacattct aacagctttt tagttaaaaa ttattcacct aaagcatctt
    22141 ttcttaactg ctcaaccttg tcagttcttt cccaaggtaa atctatatca gttctaccaa
    22201 agtgaccata agcagcaacc tttttataaa gaggctttct taaatctaag tctcttatta
    22261 tcgctcccgg tcttaaatca aagtgtttat ttactaattc aacaagtttt tcttctgaaa
    22321 ccttgcctgt cccaaaagta tctataaata tagataatgg tctagctata cctatagcat
    22381 atgcaagttc tatctcacat ttgtctgcaa gaccagccgc aacaatattt tttgcaacat
    22441 atctagctgc gtaagcagca gatctatcaa cttttgtagg gtcttttcct gagaaagctc
    22501 ctccaccatg tctagaatat ccaccataag tatctattat tatttttctt cctgtaagac
    22561 ctgtatctcc ttgtggtcct cctataacga atcttcctgt tggatttata taaattttag
    22621 tctcttcatc aagtaattct gctggtataa cttccttaat aacatgatta attaaatctt
    22681 ctcttatttt atcatttgat actgtttcac aatgttgagc tgatataaga actgtatgta
    22741 ctcttacagc tttacttcct tcatattcaa cagtaacttg agtttttcca tctggtctta
    22801 aataatcaac taaacctgtt tttctaactt ctgtaagtct tcttgataat ttatgtgcca
    22861 aagatatcgg aagtggcatt aattctggag tctcattaca tgcaaatcca aacattatac
    22921 cttggtctcc agctccaact ttttctattt cttcttctat ctcttctcca gtcttacttt
    22981 ctagaccctc atcaactccc atagctatat ctccagattg ttcatcaata gaagttataa
    23041 ctgcgcaagt atcacaatca aatccatatt ttgctcttgt atatccaatt tctcttactg
    23101 tttctcttac taatttagga atgtctacat aagcagatgt acttatttct cctgctacta
    23161 aaactaaacc agtagtaact gtagtttcac aagctactct agattgtggg tctttttcta
    23221 ataatgcatc taatattgaa tctgatattt ggtcacatat cttatcagga tgtccttctg
    23281 taactgattc tgacgtaaat aaatgtcttg ccatttttat tcccccttat ttattcttaa
    23341 tttttattat aggttttacc ttgtttttaa ttttagaaat ttactcaaaa taaaaaaacc
    23401 tctcctttat aagaagaggt taataatcgt aattacatac taattaactg acctcatctt
    23461 tcaagacttt tgtcttgtag gaattagcac cttttcccat ttgggtggtt gctgggcttc
    23521 acagggccta tccctcagcc tctcttgata aggtaatcaa gatttaagtt ttgttttact
    23581 ttactagctt actatttttt taaaattttg tcaatatttt tagtcatatt ttttctattc
    23641 ctttttttgt aagacaaata aaattttcta tctatctgaa atttaaagtt aggtcttttt
    23701 aatgcttttt attcatatat ttttttgaag ggggtgttcc tattgtattt agctaaagta
    23761 aactacagcg atgatgatgt tatacagatg ctaagttcag ttctaaaaac tataattaat
    23821 gaaaatacga ttgttgtttg cataggaaca gacagggcta taggtgatac tttaggccct
    23881 ttagttggaa caatacttaa gaacagtaac tttaaatacc ccgtttacgg tacactagat
    23941 aaccctattc acgccttaaa tatatatgag tctttagata ctataaaaaa tacacatata
    24001 caaggtaact tcttggctat agatgcttgc ttggggtcgc agagtaacat aggtaatata
    24061 cagattagag aaggacctat cctgcccgga aaaggtgttg gtaaaaaact acctcaaata
    24121 ggaaattatt ctatagtagg aattgtagat aaaattgatg aaaataataa actttcattt
    24181 aataatatac gccttagctt tatattagat ttagctgaaa caatagcctt ggctttatta
    24241 gtatccacct aatctaaata taaactaaat gataaaattc tttatataaa aaggtagata
    24301 ttttagagta tctgattaaa tactccaaat tatctacctt tttactttta tatttcaagt
    24361 ttttattttt ctactaaagc aaacattaat tcacctttac agacaagttc ttcacctaca
    24421 tatgccttag catctgcctt agctatattt tttctaagag ttgtcatttc aacttccata
    24481 gttaatgtat ctcctggtct tacttctctc ttgattctaa ctttatctat acctgcaaat
    24541 acacctagtt ttcctttgtt ttcctctaca gacatcattg ccactccaca tacttgtgct
    24601 agagcttcta ctattagtac tcctggcatc aaaggatatt ctggaaagtg accttggaaa
    24661 aatggttcat taactgttat attctttata ccaacagctc ttttcccaac ttctagctct
    24721 gtaatcttgt ctactaataa aaatggatat ctatgtggta ttaatttttt tatttcatct
    24781 atatttaaca ttctaaactc tcctaattat tttcttttga aagacccacc agtttcaagt
    24841 aagtctattg aacctaaagc tattcctgta ccttttgcaa cacaagataa tggttcatct
    24901 gctattgtaa cagttattcc tgtcctctgt tctattattt tatctaatcc atataataat
    24961 gaacctcctc cagtcattat tataccgaca tctcctatat ctgctgctaa ctcaggaggc
    25021 gttttttcca aaacagcatg agtagcaact actatttgct caacacattc tctaagtgct
    25081 tccaacattt ctgttgaatt tatagttata gaactaggaa gtcctgtaat taaatttctt
    25141 cctgttatct tcatatattt ttcttcttct ctcttaaatg cagaacctat ttcaaacttg
    25201 attttctctg ctgttctttc tcctataaga agattgtgtt gttttttcat atatttaact
    25261 atagcatcat caaatctatc tccaccaacc tttatagatt cacttacaac agctcctcct
    25321 agagatatta cagctatatc tacagtacct ccaccaatat ctataatcat attaccatta
    25381 ggctgtgata tgtctacacc agctcctatg gcagctgcta ttggttcttc tatgatatat
    25441 acttctctag ctccagcttg tctcgtagca tcttctactg ctcttttttc cacttctgtg
    25501 acacctgttg gtacgcatac cataattctt ggcataaaaa atcttccaaa tcccttttta
    25561 tcaactatct tatctataaa agattttaac atcttctcag ttacatcata atctgaaatt
    25621 acaccatctc ttaaaggtct tatagcgact atattacctg gtgttctccc tatcatcttt
    25681 ttagcttctt ctcccacagc taaaacagaa tctgttcttt tgtctatcgc cacaacagat
    25741 ggttcttcta aaacgattcc cttaccacta acataaacaa gcacatttgc agtacctaaa
    25801 tctatgccta tatcagctcc agccattttt tcaactcctt tatttataat attagattat
    25861 attataagcc tattttaata ctcctataat aaagcatata caaaaatctc gctttaatca
    25921 agagaaaata aatcttttga gagtattatt attttgctat ttataattac ttaatatatt
    25981 tctttttata atttaattca ataaaaaaag taggttttta acctacattt ttttatgttc
    26041 cttctcatat tttaatttcg ttgctaatcc ccctctaata tgtctctctg atttattttt
    26101 gtccagcaca tttttaactt ctttggcaag ggatggattt atttccttca atctttctgt
    26161 aacatcctta tgaattgtac ttttacttac tccaaatgtc ttggcggttt gtcttactgt
    26221 agtgtttttt tcaagtatat attttgctac cactatggcc ctttcctcta tatgagatct
    26281 caaagattac ccccctcaca taatagcttt aatgatatat atgtaaaaac accaaaaaat
    26341 ataacaagct taaccaaata tgattaaact tgttatacta ttacttaatt agactcattg
    26401 ggtctataga tttttctcca ctatacgcct ctaaatgcac atgtatacct tcttcacttt
    26461 ctatttgaga agtactacct acagtaccta gagattgtcc ttctgttacc ttctgttctt
    26521 tcttaacact aacattttta tctagatttg aatatactac tacaatgtta ttgtcacttt
    26581 ttattttgac tgattgtcca tattcttcat catcaaatac atctactact attccgttta
    26641 atagagattt tacttctttg cctttagtac aactaatatc tacaccttta tgagtttccc
    26701 aaacatcaag tgttttagaa taactaggct ctttttctga atatcctctt attactttat
    26761 ctccaatgta atttaatttt gttgtactcg atttattttc ctcttttgac ttagcttttt
    26821 ctaagttttg ttttgaatcc gttgcagtag ggatagcatc tttcttgtct ttttcaatta
    26881 aatgtatttc ctcttcacta tctttattag cattttccat aattccttta ttagaagcca
    26941 atttatctac attattattt gtaaaccaaa ctccacctac tgctaataaa caaacacata
    27001 caaacaaaga taaataaaaa ccatcttttt ctaacagctt tttcttcatt ttgcacctcc
    27061 aaatatattt ctacatttgg attattgacg tgtttttggc attttataca attattagta
    27121 tatatctttt atttttgtat ctgtgtagta atgtgataat atatcataat atttataacc
    27181 ctcttctgcc attccttctg ctccccactg gctcatacca acaccatgac catatccttt
    27241 taccacgaaa tctatgtatc cttctccaaa ttttatgtca aagtttgcag agttcaactt
    27301 aaaaacagtt ctaatgtctt ttccagtcaa ttctttattt cctaatttta ttttttctac
    27361 agttccggca tcacttcttt tagttattga aacttgttta cttaaattat tcacatctat
    27421 cacaatagtg ctatatgccc ttctaagact tttaacaaaa tctgtgttac ttattttaag
    27481 tgttgacgca aatttagggg aatacttatc ataaggactt tccacagact ttaaataagg
    27541 atattttgca gaaaatactt cttcactatt ttctgttttt cctgaagatg tagaaaaata
    27601 aagaggaagt atcgccttat cattataagt tattatttga cctttagttc ctctaactgc
    27661 ttcttggatt ttggaatatt tattcttaat ccattcttca ccatttaatt ttttaagagt
    27721 atcataactc ttatattctt gacaatgttt ataatcagta catactactg catttttatg
    27781 tttactagat ttaccatgtt cctgtttata tactacatat gttctagctg ctacagattg
    27841 agcttttaaa gcttctatat caaactctga agacatctca ccagctagta ctccacataa
    27901 atagttctcc atgtccatct tttcggtttt ccctgttata tgattatata cattgataat
    27961 tggtgctttt ttatttacag tttcatagtt cactgtattt ttttcttctt tattaccttt
    28021 gtcttcaata tcactttttt taatagtttt gtttatagat actgggctct ctggtttttc
    28081 agttaattct acatttttat aagaaactaa agttataagt gatggaacta acacagagca
    28141 agtcacaaat cctaacaaaa caaccaatgg gttcttcata tactctcctc cttagctttt
    28201 aaaaccttgt atttaagtat atgaacaacc ctattaattt ataactttct tctataaacc
    28261 aattattaat cttctactat ttcgatttga ccacctagtg ctgttatctt cttatctata
    28321 tcaacataac ctctttggat atggtatatt tctcctattt gagtttctcc ttctgctata
    28381 agaccgcata aaataagggc tgcccctgct cttaaatcag tagcgtttac tgctgcaccg
    28441 tgtagctcat ctacaccatt aacaaccgca cttcttccat ctatttttat attagcaccc
    28501 attctattaa attcagcaac atgcataaat ctgttttcaa aaacagtttc tataactaca
    28561 ccagttccat tggctacagt aagcatagcc ataaattgtg cttgaacatc tgtaggaaat
    28621 cctggatgtg gtaaagtttt tatatctatt ggttttaata cttttggacc tacaactctt
    28681 acagaattgt ccatttctgt tatttcacaa ccagcttctc ttaatttagc tacaactggt
    28741 tttaaatgct ccattaaaac attttcaaca gttatatcac cttttgtcat agctgctgca
    28801 accatataag ttgctgcttc tattctgtct ggaattacat tatgttctgc tccttttaac
    28861 tctttaacac cttttatttt tatagtatta gtacctgcac ctttaacatc tgctcccatt
    28921 tcgttcaaga agtttgctaa atctactatt tctggctctt ctgctgcatt ctctattatt
    28981 gtagtccctt ctgctaaaga agctgccatc attatgtttt cagttgctcc aacagatggg
    29041 aaatctaaat ataatttatt tcctactaac ttttctgttg ctgcttcaac aaaaccatgg
    29101 tccatttcta tctttgcacc taaagcttta aatcctttta aatgtaaatc tataggtctt
    29161 gttcctatag cacatcctcc tggcatagaa attttcgtac tattaaatct agcaagcaat
    29221 ggtcccataa ccaaaaaaga agctctcatc tttcttacaa gctcatatgg agcttcacaa
    29281 gtttttatat tgcttgcatc aacagtaagt gtattttctt tatattcaac ttctgctcct
    29341 acatgtctta ataaatctga tataacatga acatctctta aatttggtac tccatttaat
    29401 gttgattttc cgtttgctaa taaagtcgct gctattattg gtaaaactgc attttttgca
    29461 ccatctattt taacactacc tttaagtgga ttactctttt ttactattat cttagccata
    29521 ttttttgatc tcctcataat taataatcta attttataat tggtgtggca atatatatga
    29581 gtgtttttcc gtcatcctca ttatatttaa ttcctatatt taaattaatt ttttccccta
    29641 aatattctaa ttcataatca tttaatactt tactatatgc tgttatagat aaaaagttta
    29701 cgtctttaac ttcatctatt tgcttagcac tcatattata taatattttt tgtaaaatat
    29761 catcatattt gttgttttgt aacctttttg tgtattctcc aacaatacaa gcagacacat
    29821 ctacttgaga tgaatattta tttaacacat tttctacgtt actataaata acctctttat
    29881 gtttatatac tttgttatcc aatatgtcaa ctactatgta actttctttg ttatttttat
    29941 ttattccttt tatagaaatt tctttatcgc taccttttat agtagcacat aattgtattt
    30001 tatcgccaga ccctttttga ttccaattta ttcctttttt atccaacttt agattgttaa
    30061 ttatttctat aaaaatatta tgaaattctt tatttgataa attatgattt ataacagaat
    30121 taattttaac attataaaat ttaaaatttg cttctgatat ttcaagagct ttacttaatc
    30181 tattgtatct agtatttata cttgtatctg cataagatat tgtcattcct ataatcatta
    30241 atataaaaaa gcttatagct aattttactt ttttcatagt aaaaaatccc ccttaaatat
    30301 gtttttctgt ataatagaat tattaacaaa tttaagagag atatactttc tcaatatgta
    30361 tttttataca tttttgtatt tttcatctaa taatctcttt caaactcttc aatctcaatt
    30421 ttctctatcc ttctcctgga cttgccaatt tcttatatgt atcaaataca tcttgcatta
    30481 attcttatat attctcttct gtaatataaa ttacttagtc tatacttaaa tctataatct
    30541 ataatctata atctataatc tataatctat tcatatttta ccaacaaaat aaacagataa
    30601 tactttttta tcaatttcca tagctccaaa tgaacatacc taagtacact tcccaataca
    30661 caatttacac actcttccta ataaatcaaa aacacctttg ttttaaacaa ctctattgca
    30721 ctttcaaaaa ctttatttgt gttctcccac ttcaattaaa tttgaattat caagactcca
    30781 gcccatccat ccatcactat aaagggaggt attttccact cctatgacat ttgcatatgt
    30841 taatacttca gcagcacgcc atccacttcc acacataaat ataagctgtt tattcacatc
    30901 tatattttcc ttgtcccaca tttctaaaat ctcatattta tttctcattg tattatcaat
    30961 attacgatat tcttctagtg aaacactatc actacctgaa tgtccatata aagctcctgg
    31021 tatacgccct tttttatcat aatatgtata tccagatact tttcctatat gttcatccca
    31081 agtacggtta tctactagta taaacttatt tttttctttt agtttttgtc ttaattcaga
    31141 tgtagtaaca attaattgtg agttaactgg tatatctgct ccaaaattag tacatgaatg
    31201 ttttggatta ctaataaatt ctaattcata tcctgctgat aaccatgagt ttgttccacc
    31261 attcaaaact cttacatctt ttactcctat atatctcaat ataacagcta gtctataaga
    31321 tgccataggt gtactacttg aaactattac agtatcatcc tttgttaatc catagtctaa
    31381 agcaaactta gttaagttat catcattatc aagcatccat gttggaggag gttctataat
    31441 atcagtattt acatgaacac ttgtaggtat atgccctttt gtatacgatt cttctctcca
    31501 actcgcttca atcattttta tattctttga atcttcaaat gtctcaggaa tttttccatc
    31561 taatatatct tttattataa aagcaggaac tatcatttga taatttttat atctttccat
    31621 tggaagattt tcgtcgtctg cccattgttt aatatcatat ttatatagat atttatatcc
    31681 ttttttagat aaataatctg ctacaaccaa agcatctttt ccatttgcat catacaatac
    31741 tatattttta tctaaatcaa ttcttttagt tttcaatgct tgttctaaca cttcatcttt
    31801 tctatctgag tatacactta accaattagc agaaaaatct actgctcctt ttatatgtcc
    31861 acctcttttt actccatcta acttccagcc attataagca tcatttaagc gagtatcaac
    31921 tataacccaa gcgttatctt gcactttttc ttgtaaaact tcagtagata ttgccttaag
    31981 attaacaaaa taatcttctt tcttttcttt tttaaaatca tttgtcataa tttatactcc
    32041 caatttaaaa aaatttttat ttgaaattat tttactaaaa taaagtaatt ttcttatact
    32101 ctattagctt cattatattc ttttataaat agttccttat attcttattt taaactactc
    32161 actttatctt atcacaaggt tactaatacc ttgtatttta atttttctca taagctatta
    32221 tttttttatc tgatattgac tatatgttta ttaagtactc actatatttt atatgaacta
    32281 tatttatcgt tccctatacc tggtatggtc tatatattta tcattttaca gatgatttaa
    32341 tatcaatctt tttataaaat ttattatagc aaaaacaaaa caaatttaat atttatctaa
    32401 aaaactcatc ctataaaata caaataacag gatgagttct tttcaataaa tattatctat
    32461 ttaattttta atctgttttt attcaactgt tactgattta gctagatttc ttggcttatc
    32521 tacatcgcat cctcttagta cagagacatg ataagcaagt aattgcattg gtacaactgc
    32581 taatattggt gataaaatat catctgaatt tggtatatag attattttat cagcagcttt
    32641 ctcaacatcc ttattatggc tttgagctat tgctactaca taagctcctc ttgctcttac
    32701 ttcttccata ttagaaacca ttttttcaaa taacttttct tgagtagcta tggcaattac
    32761 aggagttcct ttttctatta aagctattgt tccatgcttt agctctcctg ctgcaaaagc
    32821 ttctgcatgt atatatgaaa tctcttttaa tttcaatgaa ccttccattg ctaagctata
    32881 gtctatacct cttcctaaat agaatgcatg ttcagaacta acaactgttt tagcaacttc
    32941 ttttatatat tcttcattgt ctagtatttc ttgtattttt gatggtattt ctttcatctt
    33001 agaaatcata ctatcataaa attctctagt tattgttcct tttttgatag caaagtctaa
    33061 agctatcatg tataaagaag ttatttgagt agtatatgcc ttagttgatg caactgctac
    33121 ttcaggtcct gcccaagtat agaatacatc atctgattct cttgctatag atgaacctac
    33181 aacattagtt attgaaagta ttcttgctcc tttagccttt gaatctctta atactgctag
    33241 agtatctgct gtctcaccag attgactaac taatattact aatgaatttt catctacaaa
    33301 tgggtcgcta tatctaaatt ctgatgctat atcagttatt actggtatat ttacaaattt
    33361 ttctatggca tacttaccaa gtaatccagc attatatgct gttccacaag ccactatata
    33421 aactttattt attttttcta agtcttcttt agaaatattt atactgtcta gaattatatt
    33481 tccattatca tcaagtcttc tttctaaagt ttctcttact cctgttggtt gctcatgaat
    33541 ttcttttgac ataaagtagt catatccacc tttagacgca gcttctacat cccaagttac
    33601 atgaaatact tctttttcaa caagatttct attagagtca taaactgtta cattttcgtc
    33661 ttttagatga actacttctc cattttctaa gaaataaaca tctctagtat attttaataa
    33721 tgcaggtata tctgatgcta taaagttttc accttcacca acaccaacta caagaggact
    33781 atcttttctt actgcaacta attcatttcc atgttcttta catataacac ctaatgcata
    33841 agctcctctt aattttgata tagttttgta tacagcatct aataaattac cctcatagta
    33901 tttatctact aaatgtgcta ttacctctgt atctgtttga gattcaaact taacaccttc
    33961 agaaattaat tcttctttta tttccatata gttttctatt atcccattat gaacaactgc
    34021 tattgttttg gcttgattaa aatgaggatg tgagtttaca tcagatggtt ctccatgagt
    34081 tgcccatctc gtatgcccta tacctaaatt tccatcaata ggattctttt ctaagtcttc
    34141 tgcaagtaca gaaagtctac ctttaaactt tctaatatta agttcttttt cattagaact
    34201 attaactgct actcctgctg agtcataacc tctgtactct agcttagaaa gcccttctac
    34261 tataacttct gctgcctttc tacttcctaa atatccaact attccacaca ttatttgtgt
    34321 cctcctaaaa atattattta tatattttaa gagttaggtc atataagttt actttggctt
    34381 ccctttgtcc ataggattgc tcctattatt taaagaagtc tttcgatggt aaacacaccg
    34441 cagagcatcc gccgacaatt cgattaactc tgacctcgtc aacttagttc ctatattact
    34501 caataattat gtctataact aagttctggc gcttagaaag gtgtcctcag tatttatatc
    34561 tgagaatacc ctttaaatta cgataatttt tcttgtataa gattagctaa gtttgttgct
    34621 aattctttta tttgaccttc ctctttaccc tctaacataa ctctaactaa tggctcagtt
    34681 cctgatggtc ttatcaaaac tctaccattt ccgtctaaaa tagattctat tctttctatc
    34741 tcagttttta tttctggata ttccatatat ttgttcttat tttcattttt tattgttgca
    34801 tttactaata cttgtgggta ttggctcatt attgatgcaa tttctgataa aggtttcttt
    34861 tcttgcaaga ttatatttgc aagtattaga gaactaagca ctccatctcc agtagtgttg
    34921 taatctaaaa atatcatatg tccagattgc tctccaccaa gattatatcc atttttaacc
    34981 atatcttcta aaacatatct gtctccaact gctgtagtag ataaatttat tccattttcc
    35041 ttagcagcta ttgtcaaccc tatattactc ataactgtaa ctaccaaagt atcttgtgcc
    35101 aatttattat ttttctttaa atttaaggca ctcaatatca ttatatggtc tccatcaaca
    35161 atttgaccct tttcatttac tgcaattaat ctatcagcat ctccatcata tgcaagacct
    35221 aagtcagcat tatgttccaa aacagccctt tgaagttgtt ctggatgtgt agagccacat
    35281 ttatagttta tattatttcc atctggtgaa ctatttattg aaattacact tgctcctagt
    35341 tcatcaaaca ctattggtgc cactttatat gacgcaccat tagcacagtc caaaactact
    35401 tttaatccct taaagtcagt gcttattatt gattttaggt agtctacata gtctctttgt
    35461 gcacaatgtt catgtaattt tcttcctaca ttttcaccaa ttggaagaca atcaattttg
    35521 tctatatcgt ctatatattc ttctattttc aattcaattt catcatctaa tttatatcca
    35581 ttcttattaa agaatttgat tccattatat tcaacaggat tgtgagatgc agatataacc
    35641 acaccacaat cagcaccata ttttctagtt aaatatgcca ctgcaggtgt tggtattatt
    35701 ccaacagtaa taacatcaca tccaacagac ataagtcctg ctattaaaga tgcttccaac
    35761 atatcacctg atattctagt atccttacct actactactt ttactctgtg gtctccttga
    35821 gctaagacaa atccaccagc tctaccaagt ttatatgcta aatcacatgt tagttctgta
    35881 tttgctactc ctctaactcc atctgttcca aaatactttc tcactaaaga ttctcctttc
    35941 ataattgaaa agcttgtaat ttgtattata tttttatagt tcataaaata tcattatagg
    36001 cttaatttca gtcataaaaa tattttaata cttatcagca aaaaagacaa tagaaataat
    36061 tctattgtct tttttttgac atctttttct ttattagtta gttgctttag aacatgtagc
    36121 atccatacct ttttgtaaag cttctctgtt taatggaacg aatttttctt ttctaggtcc
    36181 gaaaactttt ttgaatgctt ctagtacaga ttctatatct acagtatcag atacttttaa
    36241 gtaagcgcct aacattatca tgttagcaac tttatcattt cctaattcag ctgctaattc
    36301 atttgctggt atataataaa catctacatc tgttctatca actttttctt ttattaaaga
    36361 actatttact attatttttc caccaggaat aacatcatct ttgaattttt caaatgctgg
    36421 tatatttaat aatatagctg cagttgcatc atctgttata agtggagaac caactggctc
    36481 attagataca gttactgcac agtttgctgt acctccacgc atttctggtc cataagatgg
    36541 taaccaagaa acttcttttc cttctagcat tcctgcataa gtaagtaatt gtcccataga
    36601 cataacacct tggcctccga agcctgcaca tattactcta gctgtagaca tttacttcac
    36661 ctcctcaact tcaacatttt tgaaatttcc taatggatag tatggtatca tgttgtctct
    36721 taaccattgt aaagcatcgt ttggagctag tccccagttt gtaggacaag tagataatac
    36781 ttctactatt ccaaatccta atcctgcttg ttgaacttgg aatgctttct ttatagcttt
    36841 tttagcttgt ctaacatgag ctggagtatc aactgctact ctttctacaa atacagctcc
    36901 tgttaatgta gctaacattt cacttactct tattggataa ccttgagttt cagcatttct
    36961 tcctgattga gcagtagtag ctctttgtcc aactaatgta gttggagcca tttgtccacc
    37021 agtcatacca taagttgtat tgtttacaaa tatagtagtt attttttctc ctctagcagc
    37081 tgcatgaact atttcagcag taccaattga agctaagtct ccatcacctt gataagtgaa
    37141 tactgtattt tcaggatgaa ctctttttat tcctgtagca gcagctggcg ctctaccatg
    37201 agcagcttct tgagtgtcac agttaaaata tttatatcct aaaactgaac atccaactgg
    37261 aacaacacct acagcatcac ctaatactcc tagttcttct aatacttcac caactaatct
    37321 atggattata ccatgtgtac atcctggaca gtagtgagtt tgagtatctt gtaatccttc
    37381 agttttctta aatacaacag ccattatcta gcacctcctg caaccaattc tccggcaata
    37441 atttttttag ctttttcaac tatttctgct ggtgttggag tcattcctcc tggtcttcca
    37501 tggaagtaaa ctggtaattt accttctact gccattttaa catcttccac catttgtccc
    37561 atactcattt caacagtcaa tatgtttcta gcattaggaa tttggttgaa tgcttcgaat
    37621 gggaatggcc ataatacttt aggtcttata agacctgctt ttattccttc ttctcttaat
    37681 atatcaattg cgctttttac tactcttgaa gtagttccat aagctgcaaa tacaaattca
    37741 gcatcttctg ttttgtacat ttcgtattgt acttcattct tttccattgc atcaaatttt
    37801 tcttgtaaat gccaacaatg gtcttctagt acttctggct ctaagtatag agagtttatt
    37861 acattaggtt ttctttttcc tttagttcca acagttgccc aatctttagg aggcaattct
    37921 cttttcttct caggagctct aaattcaact ggctccatca tttgacctat cataccatct
    37981 gcaactacca taactggtga tctgtaataa tctgcaacgt caaaagcttc cattatcata
    38041 tcaacagctt cttgaactgt agctggtgcg aatactggag ttctgtagtc tccatttcct
    38101 ccacctcttg tagacataaa gtagtcagct tgtgaaggtt gtatacttcc aagtcctgga
    38161 ccacctctca ttacattaag tacaacacaa ggaacttctg ctcctactgc atatgttata
    38221 ccttcttgct ttaaagcaac tcctggtgaa gatgaagaag tcataactct tgctcctgca
    38281 cctgctccac cataaaccat gtttatcgca gaaacttctg attcagcttg aacgaacgct
    38341 ccacctattt taggtaactc tcttgataaa tattctggta attcactttg tggagttatt
    38401 gggtaaccga agaaatattt gcaaccagct tctatagctg cttttccaaa tgcttcgtta
    38461 cctttcataa gtatcttagc cattaaaatt cccccttaaa tattaattaa attaatctct
    38521 ttctactgtt ataactacat ctggacacat agttgcacaa cttgcacatc ctatgcattt
    38581 ttccatttca aaaacagttg ctgggttata cccttttttg tttattacat ttgagtctaa
    38641 ttgtatgatt tttactggac atgcttcaac acacaatcca caacctttac aacgttcttg
    38701 attaaaagat acttttcctt tagccattta cctaagcctc cttaaacttt ctatatattt
    38761 gagctcaaac tacatccagt cttctctcat atataatttt ataggaaaaa tttctccttc
    38821 taaatctttt ggtaactgtt caacaagatt ttctaaacat gcaacatatc ttatcggtat
    38881 attacatttt tcagaaactt cttttgcaac tttctgacct cttaaaacat catcaatagt
    38941 agtaaaccta ataagatgag tattattaat tagacctgtt acctttaatt tagaagattt
    39001 ttctatttct tttatatatt ggataacttc ttcagaagtt tgagttttct ccctatttgc
    39061 atttattaca aaaagcatat cataatcgcc ttcttttaac aaatgactaa atcttcctat
    39121 aaccctagca cctacattat ctccacctaa atctataaca gaattgtatg aatggtctac
    39181 cataggagac attacctctg ctgatactgc tggtaaatct aatgttggag cattaattga
    39241 gctatctatt ggttgtatac ctaaggattt cattaactct ttcttttctc tagttctaaa
    39301 gtatacattt accacatcta aatcggctat agcaactttg ccagaaacca tatctcttaa
    39361 cttaacaaca tagttcatcg caaattcact tttaccactt ccatagtgac cagttatgat
    39421 tcttattctt ttatcattag ttatcataaa cttgagcctc ttcttcacca gttaaaactc
    39481 taagtccacc ttgagctaat gcaatcattt catcttcacc tggataaact tttacatctg
    39541 ctataaattt aactctatct gcaatcattt ctgtaaacat ttttgaatat gcgattccac
    39601 cagttaataa tattgctttt acatctccct taagaactgc agcactagct cctatttctt
    39661 tagagatttg atatgccata gcttcatatg ctaatttagc tttttcatca ccagcttcaa
    39721 ttctttcttc aacttctcta gcatcattag tgtttaagta tgcaactaat ccgccattac
    39781 cttttatttt ctttttaatt tcatcttgag tatattttcc actaaagcac atttttacta
    39841 atgcacctac tggtagtcca ccacttcttt ctggagagaa aggtccttct ccatctaatg
    39901 cgtttgcaac atctactatt ttaccatttt tatgagctcc aacagaaact cctccaccca
    39961 tatgtgcaac tataagattt atatcttcat atttcttgtt tatttctcta gcatatcttc
    40021 ttgctattgc cttttgattt aaagcatgta ctacacttgc tctacttatt tcaggcatac
    40081 cagaaattct agcaacatct tctaattcat ctacaacaac agggtctact atgtatgaag
    40141 gaacatttac ttcttcacct atttgttttg ctattattcc acctaggttt gaagcgtgtt
    40201 ctcctaaaac tcccactttt aaatcttcaa tcatagcagc acttactgaa taagtaccac
    40261 cttttatagg tttaagaagt cctcctctac ctactacagc atctaattca gatgttttta
    40321 ctccaccttc ttttagagct tcttctatta cttgtttacg aaattcaaat tggtcagaca
    40381 ccttctcata ttttcctatt tcttctgaag aatgtcttaa agttttttca aatactaaat
    40441 cctcattatc aaatacagct attttagttg atgtcgaacc aggatttatt gttaagattt
    40501 taaatatttt gctcatttgc ctatttcccc cttaaaatta tgcctttgct gccattaaaa
    40561 cacctaaagc tattgagttt agtttagttt cttcactgtc tgctctagaa gttaatatta
    40621 ttggtgcttt agccccaact ataactcctg cattttttga ttttgagaag aataccaaag
    40681 ctttatataa tatgttacca ccttcaatat ctggggctaa taatatatca gctcttcctg
    40741 ctacaggatg atttatacct ttatgtttag ctgcttctaa agatactgca ttatcaattg
    40801 caaaaggtcc accaaccata caacctttga tttctcctct ttcatacatt tcttctagtt
    40861 ctttagcttc aactgtatct ttcatttttg gatttacttt ttcttttgcg catattgcag
    40921 caacttttgg ttcacttata tctaatgaat gtgctactgt gcaagcattt tctatgattt
    40981 gctttttagt atttgtatca ggagctaagt tcatagctgc gtcagttacg aaaaataatc
    41041 tatcatatcc ctctacatca aatactgcta cgtgacttaa tacatttcca gttctaagac
    41101 ctacttcttt atttaaaact gcttttagta ttattgatgt gtctactaag cctttcatta
    41161 ccatgtcggc ttttccttgt gaaactaatt caacagattt tagagatgct tctgctaaat
    41221 cttttatatc tatcagttca taattttcga tatccatgtc tatgcttttt gcaatttctt
    41281 tagtcttttc tatatctcct actaaaatgg catttgctat tttttctttt ctagccattt
    41341 caactgccat taaaacttct ttatcttggc aacatgctac tgatatagtt ttaggtcctc
    41401 tttcttttgc aaacttaatt acttcttcaa aacttctcat gcatttccac ctcatcttga
    41461 attttttgaa atagaacttt ttatatctca attaaattat aactcgttca gcaacgtttt
    41521 cctagagata attgaaatat ttaattcaaa atatttattt aaatctattt atcagtcttt
    41581 tttcatatat ctagcgcttt ttagatttaa caaatatctc attgtcctta ttttataata
    41641 taactaaaat aaacaagatg caacaaaaac ataaaattaa caaacttaat tttataggtt
    41701 tttttgtcgc atcttgtaac aactaaatat tcgaaatatt tgttcattcc acttcaacaa
    41761 tattaggttc tatatttatt tttttaaatt gatatgttgt ttcatatttt attttatatt
    41821 tactatcttc ctcaggacct tgtgataaat caatatataa tgaaatatct tttttattta
    41881 acttactcaa atcctccatt gactctacaa caacttttat atctacatca gaaattacat
    41941 ttggattatc catatttgtt gggatatttc ttaattcaat atctcccgca ttatataaaa
    42001 attctgcact caattgtttt gtatcattta atttaattgt tatagagcta tcctcaagag
    42061 ttataccctc tggaatttct aagctaactt ctttttcttc accattagcc aaatcagcta
    42121 agtcaatagg ttttgtctta atataactta ttttgtctat atcttctttc tttcctttta
    42181 ttgtaacttg ttccttactt aatttatagt ttttcagtac atcattatct ttattatcat
    42241 ttagacttac tcttattggg acaatttttt ccttcaatag ttctatcttt gcagtcacaa
    42301 aggatgaact taatgttact ccttcaacct tagttccatc actatttaat ggtattaatt
    42361 gcaatttttt agaaaaatca tctgtctcat taccaacatc tagattagct cttacacttt
    42421 tgacttcttt aactagagtt cttggacctg atacatcgac aaattcctta ctaagttcaa
    42481 ttttatttat atttcttttt gaatttccac ttacttctac ctttatatct cttttttctt
    42541 ttatgttttt ttcaagattt actactaaaa catcatgctt aaattctaga gtaacacctt
    42601 gaggaatatt tgcttttaaa tatacttcct tctgtccttg tattgggtct tcaatggttc
    42661 catatacatg tatatcttct tttttaacac tttgtacctt tgataaacta ccttttatat
    42721 atacatctgc tgttaatttt tcgtctggat atataacaag atttttatcc tttaaatcac
    42781 tcatattact tatagataca ggtatatctt caaacccttt agtttcttca gggtcaacta
    42841 cagccataac atacatccat aatgcaattg cacttaaaaa tgatattatt tttattttgg
    42901 tattattttt tagtctattt atcatttaaa tataccacct ttgaaaaaac ttcttgtttc
    42961 tgtatttgtt tttaaattac tacgtaatat attcatcatt ctttctctag aaatatctct
    43021 atataactta cctgctttag caattgatac gccacctgtt tcctctgata caatcaaagt
    43081 tatacagtca gacacctcac ttacccctat tcctgctcta tgtctagtac ccaaatcttt
    43141 acttaaatcc ttactttctg tcaatggcaa aaaacatgca gctgctttta ccttagaatc
    43201 tctaattaca acagctccat cgtgtaatgg tgtatttggt ataaatatat ttattaatag
    43261 ttgcctagaa atttcagcat caataatagt tcctgtattt attatatcac ttattctagt
    43321 ttctctctcc attatgataa gagctcctat tttttgtcta gaaagtgaat ataaagcttc
    43381 tacaatttct tctatatttc tattgagagt ttcttcagaa gtatttacat tctttccaaa
    43441 taaattaaac tttgctcttc ctatatgttc aagtcctgct ctaaattctg gttgaaaaat
    43501 tataagagca gctataactc cataatctaa agctttaaga gatatccaat aaacggtatg
    43561 caatttaaag gtgttactaa gttgagttgc taataaaaga aatattatac ctttaaacac
    43621 ttgttctgct cttgtatctt ttataaacat aaatattttg taaaaaatat acgctactat
    43681 agatatgtca attaaatcat atatactcat tcttaatatt atattaaaaa atccatttaa
    43741 aaaagaattt atatctaaca cagttttatc ctcctgtttg ataacttctt aattatttac
    43801 actaagtatt atactatata aatctaactc tatacaaact atttaacttt tatgtaaata
    43861 attctagttt tatatttgga gattgttaaa taatctctaa acacattaaa aatatcaact
    43921 taataaatac atatttctta ttaaagttag tttttactca aataactcaa accatcaagg
    43981 cgcgaataca aaatagtact cgcgccttaa ttcagtttta tgttctaaaa tatcttaata
    44041 tatctttgtt taatatatat taattattat aaattctata atccacttat taactataaa
    44101 caaatacaaa atctaaatat tacttatcat caatattatt ccattagatt catttttata
    44161 ttaaagtaat aacttatttc atatctagat tagataaatt tattttaata tcttactata
    44221 aactatcatt aaaacttatt atgtataact agatataatc tcctacagtg aaaataatac
    44281 acaataccta aagttatata tccatttcac agtataataa tttatttagt ataacttata
    44341 aatattacat taaattgtaa attcactata gtctctaatt ttttataatt tcttatactt
    44401 attgtttaga caaaaatatg ctcatgaatc tctttataaa taatttcata agttattt
//
LOCUS       DAYAOX010000007        43292 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.007, whole genome shotgun sequence.
ACCESSION   DAYAOX010000007 DAYAOX010000000
VERSION     DAYAOX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.007"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            90..968
                     /gene="purR"
                     /locus_tag="ACGI57_000309"
     CDS             90..968
                     /gene="purR"
                     /locus_tag="ACGI57_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFG9715399.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            1124..1402
                     /gene="spoVG"
                     /locus_tag="ACGI57_000310"
     CDS             1124..1402
                     /gene="spoVG"
                     /locus_tag="ACGI57_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HFG9715400.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            1615..2994
                     /gene="glmU"
                     /locus_tag="ACGI57_000311"
     CDS             1615..2994
                     /gene="glmU"
                     /locus_tag="ACGI57_000311"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFG9715401.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            3089..4039
                     /locus_tag="ACGI57_000312"
     CDS             3089..4039
                     /locus_tag="ACGI57_000312"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFG9715402.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      4877..4962
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            5124..5645
                     /locus_tag="ACGI57_000313"
     CDS             5124..5645
                     /locus_tag="ACGI57_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFG9715403.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSATTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            5796..7472
                     /locus_tag="ACGI57_000314"
     CDS             5796..7472
                     /locus_tag="ACGI57_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HFG9715404.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            7484..8692
                     /locus_tag="ACGI57_000315"
     CDS             7484..8692
                     /locus_tag="ACGI57_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFG9715405.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            8707..10419
                     /locus_tag="ACGI57_000316"
     CDS             8707..10419
                     /locus_tag="ACGI57_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFG9715406.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            10412..11320
                     /gene="pilO"
                     /locus_tag="ACGI57_000317"
     CDS             10412..11320
                     /gene="pilO"
                     /locus_tag="ACGI57_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFG9715407.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            11347..11913
                     /locus_tag="ACGI57_000318"
     CDS             11347..11913
                     /locus_tag="ACGI57_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFG9715408.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            11950..12477
                     /locus_tag="ACGI57_000319"
     CDS             11950..12477
                     /locus_tag="ACGI57_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFG9715409.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            12498..14036
                     /locus_tag="ACGI57_000320"
     CDS             12498..14036
                     /locus_tag="ACGI57_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715410.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRDISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLEKLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            14136..15218
                     /locus_tag="ACGI57_000321"
     CDS             14136..15218
                     /locus_tag="ACGI57_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFG9715411.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            15199..15981
                     /locus_tag="ACGI57_000322"
     CDS             15199..15981
                     /locus_tag="ACGI57_000322"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFG9715412.1"
                     /translation="MDIIIVNLYVFMVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            16263..16733
                     /locus_tag="ACGI57_000323"
     CDS             16263..16733
                     /locus_tag="ACGI57_000323"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFG9715413.1"
                     /translation="MVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNIVQ
                     FDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFILA
                     FLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            16747..17307
                     /gene="pth"
                     /locus_tag="ACGI57_000324"
     CDS             16747..17307
                     /gene="pth"
                     /locus_tag="ACGI57_000324"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFG9715414.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            17324..20710
                     /gene="mfd"
                     /locus_tag="ACGI57_000325"
     CDS             17324..20710
                     /gene="mfd"
                     /locus_tag="ACGI57_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFG9715415.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            20741..21736
                     /locus_tag="ACGI57_000326"
     CDS             20741..21736
                     /locus_tag="ACGI57_000326"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9715416.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            21860..22399
                     /gene="spoVT"
                     /locus_tag="ACGI57_000327"
     CDS             21860..22399
                     /gene="spoVT"
                     /locus_tag="ACGI57_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HFG9715417.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            22519..24132
                     /locus_tag="ACGI57_000328"
     CDS             22519..24132
                     /locus_tag="ACGI57_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFG9715418.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            24239..25687
                     /gene="mazG"
                     /locus_tag="ACGI57_000329"
     CDS             24239..25687
                     /gene="mazG"
                     /locus_tag="ACGI57_000329"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFG9715419.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            25776..26054
                     /locus_tag="ACGI57_000330"
     CDS             25776..26054
                     /locus_tag="ACGI57_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFG9715420.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            26126..26368
                     /locus_tag="ACGI57_000331"
     CDS             26126..26368
                     /locus_tag="ACGI57_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFG9715421.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            26430..26684
                     /locus_tag="ACGI57_000332"
     CDS             26430..26684
                     /locus_tag="ACGI57_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HFG9715422.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            26685..27218
                     /gene="yabQ"
                     /locus_tag="ACGI57_000333"
     CDS             26685..27218
                     /gene="yabQ"
                     /locus_tag="ACGI57_000333"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HFG9715423.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            27224..27508
                     /locus_tag="ACGI57_000334"
     CDS             27224..27508
                     /locus_tag="ACGI57_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFG9715424.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            27621..28541
                     /locus_tag="ACGI57_000335"
     CDS             27621..28541
                     /locus_tag="ACGI57_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HFG9715425.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDKGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            28631..31003
                     /gene="spoIIE"
                     /locus_tag="ACGI57_000336"
     CDS             28631..31003
                     /gene="spoIIE"
                     /locus_tag="ACGI57_000336"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HFG9715426.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            31354..33246
                     /gene="pepF"
                     /locus_tag="ACGI57_000337"
     CDS             31354..33246
                     /gene="pepF"
                     /locus_tag="ACGI57_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFG9715427.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            33360..34325
                     /gene="galU"
                     /locus_tag="ACGI57_000338"
     CDS             33360..34325
                     /gene="galU"
                     /locus_tag="ACGI57_000338"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFG9715428.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            34825..36429
                     /gene="rho"
                     /locus_tag="ACGI57_000339"
     CDS             34825..36429
                     /gene="rho"
                     /locus_tag="ACGI57_000339"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFG9715429.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            36582..36782
                     /gene="rpmE"
                     /locus_tag="ACGI57_000340"
     CDS             36582..36782
                     /gene="rpmE"
                     /locus_tag="ACGI57_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFG9715430.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            36927..37820
                     /locus_tag="ACGI57_000341"
     CDS             36927..37820
                     /locus_tag="ACGI57_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HFG9715431.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            37838..38686
                     /gene="prmC"
                     /locus_tag="ACGI57_000342"
     CDS             37838..38686
                     /gene="prmC"
                     /locus_tag="ACGI57_000342"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFG9715432.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            38730..39794
                     /gene="prfA"
                     /locus_tag="ACGI57_000343"
     CDS             38730..39794
                     /gene="prfA"
                     /locus_tag="ACGI57_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFG9715433.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            39923..40603
                     /locus_tag="ACGI57_000344"
     CDS             39923..40603
                     /locus_tag="ACGI57_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFG9715434.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            40632..41672
                     /locus_tag="ACGI57_000345"
     CDS             40632..41672
                     /locus_tag="ACGI57_000345"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFG9715435.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            41689..42138
                     /locus_tag="ACGI57_000346"
     CDS             41689..42138
                     /locus_tag="ACGI57_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HFG9715436.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            42155..42607
                     /gene="rpiB"
                     /locus_tag="ACGI57_000347"
     CDS             42155..42607
                     /gene="rpiB"
                     /locus_tag="ACGI57_000347"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HFG9715437.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            42643..43272
                     /gene="upp"
                     /locus_tag="ACGI57_000348"
     CDS             42643..43272
                     /gene="upp"
                     /locus_tag="ACGI57_000348"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFG9715438.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
BASE COUNT        17650 a         4487 c         7996 g        13159 t
ORIGIN      
        1 aaaacataag taatattata ccacaataag aattgttatg cattagttag tttaacgtta
       61 tataaaggta cttgtaggag gaaataataa tgaaatttaa aagaacagag agaattggag
      121 caatagttaa aatactgtct gataatccaa ataaaatata tactttaagt tattttacaa
      181 atcaatttaa tgcagcaaaa tcaacaataa gtgaagattt attagttgta aaaaatgtat
      241 ttgagaagct acatttaggt aaagttataa ctatttcagg agcagcagga ggagtaaaat
      301 atatacctaa aacttctata gcagaaaatg cagagttctt aatggaatta tgcgagagaa
      361 tatgtgacaa gtctagaata ctatctggtg gttttttata cttgattgac ctaatttacg
      421 accctacaat agctgctaag ataggaaaga tattcgcatc aaatattgag tatgtagatg
      481 cagattatgt tgtgacaatg gaaacaaaag gtatacctat ggcacttatg actgcaaaag
      541 ctatgaattt acctctagtt ataataagaa aagatataaa ggtatcagaa ggtcctacac
      601 ttagcatgac atatgtgagt ggaaacagtt ctaaagttga aagtatgagt ttgccaagaa
      661 aagctcttaa accagatagt aaagttataa ttatagatga tttcatgagg ggtggaggta
      721 ctataaaagg aatggtagac cttatgaatg aatttggtgc agaggttata ggtactggag
      781 tattcatatc tacaacgaat ccttcagaaa aaatggtaaa agattatatt tcattgattc
      841 aattggatgt taatggagat aaaatagtag tagaaccaaa cttaaagact tttaaagatg
      901 aatatagaaa tgaagattta gacgaagaag aagattcaga atttgaattt gagatagatg
      961 aagattaata aaaaaaatat ttagaaaaat atgtaaaaat aaaaagagga tatccctagt
     1021 tgttcataga attatttaaa aaacagttta tattaattta ttccagcgat aataatttta
     1081 atataaatta atttctgtca aaatcaaagg gggatatttt ggtatgaaga taactgacgt
     1141 aagagtaaga aaattaacag aagaaggtaa gatgaaatgt atagtttcaa taactttcga
     1201 taatttgttt gtagtgcatg atataaaagt tatagaaggc cataatgggc tatttatagc
     1261 aatgcctagc agaaaagtag gcgaaggaaa tttcagagat atagctcatc caataaatgc
     1321 agaaatgaga caagttttag aagatgcagt tttacaagcg tatcatgaag cattagttca
     1381 atgggaagta gctgctgaat aataataaat atataaaatg gagagagccc gaacatttag
     1441 gctcttttta aatgtagaat ttctattaaa atatgtactt tttagtaaaa aagtgctata
     1501 ttattagtga taatgtatat gttgtataca aattgttaga taaatgtatt ataatgtata
     1561 ttgattaaga gaataatacg aataaaatta aattagattg aatggagtgt atcaatgaac
     1621 tttaaagcta taatccttgc tgctggcaaa ggaacaagaa tgaagtctaa atacccaaaa
     1681 gttatacata aagtatgtgg aaaagaaatg gttaaccata ttatagatgt ttcaaaaaaa
     1741 tcaggggtaa aagatactgt cgtgatttta ggacatgaag ctgatgtagt taaagaaaag
     1801 cttgcagaag aaattataat agccatgcaa acagaacaac ttgggacagg acatgctgta
     1861 aagatggcta aagaatatat taatgatgaa gatacaatag ttgtactttg tggagatact
     1921 ccacttataa aagaagagac actaaagaga ctatttgaat atcatataga aaataaatat
     1981 catgctacag ttcttacaac aagagttgga aatccaacag gttatggtag aataataaga
     2041 gataagaagg gtgaccttct taaaatagtg gaacaaaaag atgcaaattc agaagaaaag
     2101 atgataagtg agataaactc tggaatttat tgttttaatg gaaagagtct tagagaagca
     2161 ctagatttgc taaataataa caattcacaa ggtgaatatt atttaactga tactgctaaa
     2221 ataatgagag ataaaggatt aaaagttggt gcatttgcag gttcaactat tgaagaactt
     2281 atgggcgtta attcaagagt agaattatca aaggcagaag aaattatgag acgaagaata
     2341 aatgaatctc atatggtaaa tggagtaact ataatagaca ctaactctac ctatatagaa
     2401 tctgatgtta tgataggcaa tgatactata atatatccag gtgttatgtt acaaggaaaa
     2461 acaaggatag gttcagattg tataattgga atgaattcat ctataacaaa ttcagaaata
     2521 ggtgatggta cagaaataaa aaattcaact ataattgata gtaaggttgg agaaaattct
     2581 actgttgggc catatgcata cttaagacca aagagtgact taggaaataa tgtaaaaata
     2641 ggcgattttg tagaagtaaa aaatgctatt atagaagatg gctcaaaagc atctcacctt
     2701 tcttatatag gagatgcaca tgtcggtaaa aatgtaaata ttggatgtgg agtagtattt
     2761 gtaaactatg atggtaaaaa taaatttaaa tcaatagtaa aagacaatgc gtttataggt
     2821 tcaaattcta atttagttgc accagtagtt gtagaagaaa aaggatatat agcaacaggt
     2881 tcaacaataa cacatgatgt cccagatggt gctttggcaa tagcaagaga aagacaagtt
     2941 atcaaagaag gatgggtaga aaaaaagaat caaaaagacg accaaagtaa gtaattatgt
     3001 agtttattat tttggctaaa aaatttagct cgaaattatt ataaattata ttcattagta
     3061 ttttaaaatt ttcaggaggg ttacataaat gaatactagc ggaagcgaga ttaaaattat
     3121 tgcaggtaat tcatcgaaag agttagcaca aaaaatagct gactatatag gtgtatcagt
     3181 actagattgt gaagtaggta catttagtga tggtgaaata tgtgtaaaca tgaatgagac
     3241 agtaagaggt tgtgatgtgt ttgtagtgca atctactaac agcccagtaa atgataactt
     3301 aatggagtta ttaatcttaa ttgatgcgtt aaagagagca tcagcaggta gaataacagc
     3361 agttattcct tactatggat atgcaagaca agacagaaag gctaaggcaa gagatccaat
     3421 cactgctaaa ttagttgcaa atttaataac tgctgctgga gcagatagag tattaacaat
     3481 ggatttacat gcagctcaaa tacaaggata ttttgatata ccattagacc acttactagg
     3541 tggaacaata ttagcaaatt attttaatga aaagaaaata gaagatttag tagtagtatc
     3601 tcctgactta ggaagtgtta ctagatcaag aaaatttgct aatactttaa atggagaagt
     3661 tccaatagct ataatagata aaagaagacc gaaagctaat gtatgtgaag ttatgaactt
     3721 aataggtgat gttaaaggta aaaatgttat actattagac gatatgatag atacagcagg
     3781 aacaatagtt aatgctgcaa atgcacttaa agagtttgga gcaaaagatg tttatgcttg
     3841 ttgtactcat ggtgtgttat caggtccagc tattgaaaga attgctaatt cagaaataag
     3901 tgagttaata gttcttgata ctatacaact tcctgaagag aaaagaatag ataagataaa
     3961 gataaaaaca gtagctcctt tatttggaga tgctataaga atgatattct ctaatgaatc
     4021 agtaagtaaa ttattctaat ttattgtatt tatgagagtt gtttaaaagg atataaacct
     4081 tttgagcaac tcttttttga ttttatactt atggaatgtt atttttttct tgttagaaag
     4141 gtatatatta ttgtaaaaag agattatagt ttaaaattat gtatatttaa gtaaaataaa
     4201 cctaataaag ggtatattat tcaaaatatt atgaatataa aaaatattac aaaaaagtat
     4261 tgaaaaaatt cagattatgt agtattatgt aaatataaat aaagaaagag gtgtttccaa
     4321 tgggaaggtt agaagatgaa cccctaataa ttaaatgatt cagtcacaat ctaaagtgaa
     4381 tataaaaatc tattgaaagt ttatttataa gaattgaaaa tagtatctat tctatcttta
     4441 gtgtttgtga gagggttctg gattatataa gattactgta aagagtaatt ttgcacaata
     4501 agcatatctg taaatgaaga gtttcgttta tataatgatt gtggctgaat cataactata
     4561 atagatagaa tgtttataat agatgatttt ataaaatact atattgtaat agcttttagc
     4621 tttcaaataa atattagcaa aggatggaaa tgacaatgat aaaactaaat gataatttat
     4681 aaatatctcc ttacgatata taataattgt agggagatgt ttcttttttt gtatagcata
     4741 atttttctag aaattctgtt tcaatatgta aaaagttggt atatataaat agtataaaca
     4801 gtgttgctaa aatatggtag atattatata atataagtgg atatgtatat acattatgag
     4861 ttatataatt tgaatttaat aaaatagaaa cgttgattta tgttctgtaa tgtgggcacc
     4921 ttggagcata ttgagttagt ggtgcaaccg gctatgaaat tgtatttatt tataaatact
     4981 attattttca tagctttttt tatttaaaaa atggttatat gttagaaata aatttggtta
     5041 gtacgtttaa ataattttac ttagattttt aaccagatat attgtaagta agtattaatt
     5101 aaaaaattta agggggaata aaaatgaagt taaaaaagaa taaaaaaggt ttcactttag
     5161 tggaattatt ggtagtaatt gcaattatag gtatattagc agtagtggca gttccagctt
     5221 tatttagtaa tataaacaag gctaaggtag caagtgttga gtctgattat agttcagtta
     5281 agagtgcagc attatcttat tattcagata ctaataagat accagttaca ccagatggtc
     5341 aaactggttt aagtgtttta gagacttata tggagtctct gcctgataaa gctgatatag
     5401 gtggaaaata taaattgatt aaagttggta ataaattggt attacagata ggtacaaata
     5461 ctgaaggagt tacattaaca gaggcacaat cagcaaagtt attgagtgat ataggtgaaa
     5521 ataaaatata tacaagcgct acaacaaata gtttgggaaa tccattaaca agtaatacaa
     5581 aaatagataa taacgttcta tatatagtac ttatagataa tactgtgatg gacacaacaa
     5641 aatagttaaa acaatatttt tataaaaata ttgtaaattt aatgaaaaat ataatataaa
     5701 agacaggaag aaaatcttcc tgttttttta tactattttc tttaatgttc aattatgaat
     5761 gatgatataa ttgagatagt aaaggagtga aacctgtggc taaaaaagtt agaataggag
     5821 acaaactggt cgaaaaagga tatataacag aagaacagct taaatgggca ctatcagaac
     5881 aaaaaaacag tggaaaaaga ctaggagaat ttctagtaca agaagggctt atagatagta
     5941 acttactaat aagtgtatta aaagaactct tggatataga aagtatattt ttagaaggga
     6001 cagaaataga cactttagct acaaaaatgg ttccagaaaa tatatgcaaa agatatactg
     6061 tatttccatt taaaattgat ggaaataaaa tatgtctagc catgtcagac ccacaagata
     6121 gagaagcagt tcaagatgta aggcgtatgt ctggtaaaga cgtagagata tttatatcaa
     6181 gtacagaaga tataaacaaa gcaataggtc atgcttatgc acattcagaa ataaataagg
     6241 caatgactga gtacaacaaa aatagaactg gtggagtaag agaaacagta atacttgaag
     6301 aagatgtaaa tgctgctcca atagtacgat tagtaaataa catactagaa aatgctgtta
     6361 gaatggaagc aagtgatata catatagaac aaagtgaaaa ttacatgcgt gttaggttta
     6421 ggatagatgg aatgttaaga gaatatatga ggatgaactc agcaccatat aaggcagtaa
     6481 tcagccgtat caagataatg tcagatataa atatatctga aaagagaata ccacaggatg
     6541 gtagaatcta tttaaaagta gataataaac ccatagattt tagggtttct acaatgccaa
     6601 ctaacaggga tgaaaagata gctatgagag ttcttgataa gagtaatttt atggttagta
     6661 aagaagtact aggaatagac gaacatggct ctaagatata tgatgaattg ataaacactc
     6721 cttatggtct tatattggtt gttggcccta ctggaagtgg taaaaccact acattgtatt
     6781 caatgttaaa tcaactaaat acagaaaata gaaatctatt aacaatagaa gaccctatag
     6841 agtatgaatt acctggtgta aatcaatctc aaataaatga aaaggcaggg cttactttcg
     6901 ccagcggact tagagctttt atgcgtcaag acccagatat aataatggtt ggagagataa
     6961 gagatactga gacagcagaa atagcaatta gagcttctct tacaggacat ttagttttaa
     7021 gtacattaca tgcaaataca gcagttggag caatttcacg tcttttagat atggatgtgg
     7081 aaagtttttt aataacttca tctgttttag gtgtaatatc tcaaagactt acacgaaaaa
     7141 tatgtgagca ttgtaaggtt tcatatgaag cagatattgg agaaaaaaag gctcttggta
     7201 ttgatgtaaa tgaatcagta actatttata gaggaaaagg ttgtgaaagg tgtaataata
     7261 ctggatataa aggacgtcta ggtatatttg aaatgcttga aattactcca gaaataaaag
     7321 agttaataga ttcatctgca aatcaaagag aaattttaaa gatggctaga aaacaaggta
     7381 tggttagctt aaaagaggat atagttaaaa aagttttaaa tggaaagaca acagtagaag
     7441 aaatgattag aataattttg atgactgatt aaaggatgat attatgaaaa gttttaaata
     7501 cacagtaata aaagaggacg gaaaaaagca aactgatgtg attgaagcca acgattttaa
     7561 tgaagctaga aatcttctta gaaagagaaa tcttagagtt attgaaatca aagaaagcaa
     7621 aaataaaaat ataagattat ctagtagaaa gagaaaaaaa gacttaggag ctgaccagat
     7681 aagccacttt tgtagacagt ttgctattat agtatcttct ggtataaata gtatttctgg
     7741 attagaaaca ttagctagaa ggtcaactaa cattacatta agagaagaaa ttaatcgtat
     7801 agtggcagaa ataaaagtag gttctactat tgcagattca atgctttcat caaaatcaaa
     7861 gtttccaaag ttacttggtg ctatggtagc tacaggtgaa gctacaggta aattagatga
     7921 agtattaaaa agtatggctt cattttatgc aagtgaacac agggttaaac aaaagcttag
     7981 aaatgctgca acatatccat taatagtttt gatttcttca tttataatga tttttatatt
     8041 tacaacattt atggtgccta agatgattaa ttcaattaca acagtaggag caactcttcc
     8101 acttataaca agaattgtta tggggtttgg tatgtttatg aagaagtttt ggataatagt
     8161 acttttagtt attatattat ttatctatca gtttaaaaaa tatctaaaaa cgtcagttgg
     8221 aagggctcat aaagatagag ttataaataa gatacctgtt ttaggaaaag gaataaattg
     8281 tatggtagct acacgatttt caagagcatt gtatctattt gtatcaacag gatacccatt
     8341 agttcaaggg cttgattata ttatagatag tgtaaataac accatggcag agaagatttt
     8401 agcatcagct aaagatggaa taacaagagg agaaggctta gcagaaaatt tagagagata
     8461 tacgtatttt gactctgtgt tggtacagat gatagctata ggggagcaga caggggaatt
     8521 agagaatata agtagacaga tggcagaatt ttatgagtat gaatccgaga tttatttaaa
     8581 taggatggcc tcaatgatag agcctgtttt aataattgca gttggtatta tagttgcgat
     8641 actggttgta tcaatattca tgccaatgct tagtatatat gatgccatgt aggagggaaa
     8701 ggacaaatga acaaggttta tgtatcatat tatggcgatt ctatttccat agttgagggc
     8761 agatataaac acgataagga taagttttat gtaaaaaatt ttaatgtgtt ttcaatagag
     8821 gatattgcac aagatttttc taaggaaaag tatgctctat tgagatacgc attagaaaat
     8881 gttaagttaa aatcaagaca tgtagttttt tgtttaaata caagagatgt aattttaaaa
     8941 ccccaaaaac ttcctaatat ggacaaaaag gatttagacg gatttatgaa gctagagatg
     9001 gacgaaatga tggctttgga gtcagatgaa tatgtatttt cgtatgaagt atctagtgaa
     9061 acacctgata attcagatgg cgatgaatca agtttaaatc ttattattgg agctatacaa
     9121 aaggaagagg taaatgcaat tgtagagata atgcatgaat ttgatttagt attagataga
     9181 atagatacat tatcaacagc ttacttaaga atgcttaaaa atttagacta caatgatata
     9241 atggtaacca atataagtga caatagcaca attataaata tatataagaa agatacttta
     9301 tttattagtg ataatatacc tataaagata actgctaaag atatggacat gcaattatta
     9361 aatgtagtag aagaatcaaa aggtcttatg aattactatt cgtcaagaaa ctttggtaag
     9421 gttacggata ctatattaat attaggaaaa agatattcca ataaattaat ttatgaaact
     9481 tttagctctg cattttcaag ctatgtttct caaggcctta ctgagataat ggatatatct
     9541 gatatgataa ctggagatat agatgaagat gatataaatt caattgtaga gcttttagga
     9601 agtatgatag aacctaaagg gaaaagagat tttgaaaata taaacttttt accaatggat
     9661 atacttagaa aacagataaa agctaaacaa atgaaaaaca tactgaaaat tgctccaata
     9721 gttattttga ttcttgctat accttattta tgtttaattt tttcaaataa tttagcaaat
     9781 aaggaacttg aaaatgttaa tggtgaaata agaaatattg aatctgcata ttatcagatt
     9841 ggaaatatag aaaaaatgat acagagtaaa agcgaagaaa taaaggtata tgatatgtta
     9901 attgggaaaa aggtaaagtg ggaaaattta ttggatgata tagataaaaa tactccaagt
     9961 aaagttgaac taacaagttt atcaacaact tatcaaaatc ttggaactaa taataaagct
    10021 gataattcaa aaagtaataa gactacaaca gataaatcaa gtaacaatga aacaaaatca
    10081 agtacgaaaa gtgatagtaa tggagataaa acagataatg atttaaatga agataagact
    10141 acaaatgtgt cagaagcatt aactggaaat agtgagacta atagtaataa cacagataaa
    10201 gatgataaaa ctccattgta tgataagata ccaaatgtta taaatataga aggaaatgct
    10261 aaagattcct cttatgtagg tcaattttta tataaattaa agggattata ctatttcaag
    10321 gatgttaaat tacatagcat aaaaagtaat gcagaagatt ctaactatac atttagtatg
    10381 acattatacc tgaaagaagg tgtattaact aatgaataaa gatatattta aaaaagatat
    10441 aaaagatata tttaaaatgg aagttagtct aaaagggatt cttataaagg ttataatttt
    10501 attagctatt ttatcatatg gttattttgg actttaccct caatatcaga aatatattgc
    10561 tacaaaaaat tctgtaaaac aggctactga aaaattaaat atatataaag agaaaactgc
    10621 acttatgcca aaatatgaaa aacaacttga agaagttgaa agtgaattaa aagaaaaaag
    10681 caaaaatctt ttatatgata tgcaagatgg tcttttttta ataggtttag ataaaaaaat
    10741 gaaggcttat aatattgact taaccagtta tgacgttgga aatgttaaag aatacgaaca
    10801 tttttatggt atatcaacaa caataagtgt tagaggtgat tatagaaatg tcaaaaaatt
    10861 aatggcttat ttagaagaac aaaaaaatgt tactcaaata ctagattata caatgacttc
    10921 atatatagaa ccagaaaaag aagaggaaga aacaacaaac aataaggatt taattaaaaa
    10981 tgtgaacata aataaagata caaaaatata ctgggtggaa ggcgaaaata agtggcattt
    11041 atataaggat tgtccattga taaaagaggg agagattcta aaaaatgaca aacttttaga
    11101 taggtctact aattatctat gtaatacttg tgtacttaga gctgcaacag aaaaagaagc
    11161 aaaaaaagaa gtcgtagctc caaaggcaga aggtgtagta gatgctacat ttaaatttat
    11221 aatgtacact aaagagaata ctacaatgat acttgataca gataatccat cttcatggag
    11281 accaggaaaa tataatccat ttaagtctag tgcgaaataa agctaagtaa gctttaaaga
    11341 taggtgatga gtttgtacaa aaataatgaa aaaggtctta ctttattaga agtaataata
    11401 gctgttttta tactgacaat agttttaagt atttcttata aagtgttcaa tggtataaca
    11461 tcagcagtaa aaaagcaaca gattattaca gatgctcaag taaatattaa tctaattaat
    11521 aagtatctaa atagagattt ggaaaactgt aaagaactaa ctaaaactgg ttcaggtaat
    11581 aattatgaat acaatataga gatgccagat aatgttgtaa aatatgaagt ttctatagaa
    11641 actaaaaaaa atacagaggt atattctgta acgagaatcc aaaacaatac aattgataca
    11701 gaaaatgaag ttagagaaga gataatctat aatcaaccac tagttcaaaa caataaagaa
    11761 atgaaggaaa caccatttaa aatagaaaag caaactggta aatctattta tactgtaagt
    11821 atatactata atgaatcagt gcaagaaagt cataaaaata gcaatttaaa taacaaaact
    11881 tatacatttg atgttatgtc aagaatagga taataagaat ttattaaaaa ataagtaggt
    11941 tgataaaata tgagtaaaaa gaatagtgaa agaggatttt ccttaattga agtgttagta
    12001 gctatggcta ttatgggaat tgtactattt gcatttttta atattatcaa tactaataac
    12061 aaagcaaaca ctaaaaatga cacagatata acttccttaa actatgtcca aagtgaaata
    12121 gaaaatctaa gagaaaagat aaaaagtgga gaatttgatt ttgatagttt agataaacta
    12181 gaagatggaa ctgtcgtata tgaaaaatta atagataaat caaaaaaagt tgtatatgat
    12241 aaagttctta gtgaaggtga tgtgagctta tatgatactc catatgaaaa gattacaaca
    12301 ataaaagatg aagatggtaa tttaatcgac aaaggaaata ttacaaataa gataaagact
    12361 attgtagaag ataaaagtgg tcagatatat aaaatagctg taactgggaa aagcatgaat
    12421 gactattcaa gtaaaaaaga ggttaaaatt gtaactgaaa tatttaaaga taaataattt
    12481 tacttggggg tgagattttg aggaagtgga ataaatttaa aagtgaaaga ggggcagctt
    12541 tagttttagt tttggttgta gtagcattgc tttctattgt tggattgata ttttcaaatc
    12601 agatagctaa tagaataaaa tctacaaaga ctactaatga aggtatacaa gcaaagtatt
    12661 tagcagagac atgtgtggaa aatagtattg ataaagcata tgaaaaattg tatgatgagc
    12721 ttgagaaaat ggataatgag tttaaaagtg aaaatcaaga aaaatccata tctaggtcaa
    12781 aattaagaga tatatcagat gaagatttta ataatcaaga tgaggaaaat attgaagcag
    12841 aaagacttgg atatatgaat aatataaatt tctatttaaa taaagcttct tcagacttgg
    12901 aaaaagctag tatggaattg aaaaagctat atgatttaga tatgttggac tatagagaca
    12961 ttgaatatgt agatgcaaat attatttcac atagagattc tatcttagaa atttgcaaga
    13021 attatacttc tggagatatt agtaaaataa atgaatatat tctgaaagaa gatatagact
    13081 caactacatt gattgaggca aagttagtga ataatgatat tcttttaaaa atgtttttag
    13141 aagaaaataa aattgaaaat gaacatttaa attcagcctt ttcacatact tataaggcat
    13201 tagataacat ttctttggcg atgcaaaata tgattgagta tagacatacg tttcatatag
    13261 atgagccaaa agtagaagta tctaatggga ttccagattc tcaacaatat tatgaactaa
    13321 tacaaaatcc tataataaat agtatggagt atatctggaa tagtaaatgg gatacattag
    13381 aaaaactatt ggaaatttta ccaaatcaaa ctcaaggatt taattcttta agagttcatt
    13441 tgagaaataa tgtaagaaaa tttgagaagt taagcgataa tatatctagt ggtaaaaaaa
    13501 atacagcaaa aaatttctta aagtataaag agttattgta tgaaatatca gaccaatgta
    13561 atcaactaaa aagtatgtct tatgagaaaa tacctgttaa atatgataac atggctttga
    13621 taacaacttt tgactatata caaaatgagc ttttagcaga gataaaatgc agattaaaag
    13681 aattaaaacc acaagaaatt gataaaacag aaggtattac tataaaaata cctttttata
    13741 aggctgacta cgacatgaca aaagaaggat ggcctaaatt aaaagaaaat ggttcagggg
    13801 cggagttaag cttaatggta actggtgata aagatggaat aaaagaagta gaagttacag
    13861 atggtaaaaa aaatattatt ggtttaggtg ttgaagaaaa ttcaaattca aagtataagg
    13921 ttgacgccat tgtaaacttt aatctaaata tagatacaaa tgtagttgga aattatgata
    13981 taaaagataa aattttgatt aatcatgata taagttcata caaaaaagta aactaaatat
    14041 aagaagcaac ttagtataaa taaataataa aataatttta ttatttacta ctaaaaaaat
    14101 aagtaaacta atagaagata tggagagtaa atattatgta tatttatgat ttattagagc
    14161 aaggtataag actaaacgca tcagatatac acataacagt tggaacaaat cctgtagcta
    14221 gagtaaaagg tggatttgta aaactaagtg aacaaatatt gacatcagaa gtaacaatgc
    14281 aaatggcaaa agacatagca ggggaatcta tgtttaaagt aatagaagaa catggagaag
    14341 ctgatttttc agcttcttta aaaacaggag aaagatttag ggtaaatgca tataggcaaa
    14401 aaggaaatta cgcaatagca ataaggacta taacagcaga aattccaaca tttgaaaaat
    14461 taggccttcc agagagtata aaatctttta ctgaaaagca taaaggatta gttttagtta
    14521 caggtccaac aggaagtggt aaaagtacaa cacttgcaag tatgataaat ataataaatg
    14581 aaaaacaaca aaaacatata attacacttg aagacccgat agaatatgta catcatcata
    14641 aacaaagttt agtaaatcaa agagaagtag gaactgatac agaaagtttt cattcagccc
    14701 ttagagctat tttgcgtcag gacccagatg taatactaat aggagagatg agagaccctg
    14761 aaactgtttc aatagcatta acagcagcag aaacaggtca tttagtattt tcaacactac
    14821 atacagtggg ggcagctaag acaattgata gaatagtaga catgtttcaa ccatcccaac
    14881 agcaacaaat aaaaacacag ttatctacag tttgtgaagg tgtggtatca cagcagttac
    14941 ttcctacagc agatgggaaa ggcagaatag cagcaattga attgatgttt gctactccag
    15001 caataaaaaa cctgataagg gaaggcaaaa catatcaaat tccaaacatg atacaaactg
    15061 gagttaaagt aggtatgaaa actatggacc aagatttaat ggaactttat aaaaatggaa
    15121 agattacaaa agatatggca cttagtaggt gtactgacca agagtttatg acaagaatga
    15181 taggtggagt aaactacaat ggatattata atcgttaatc tatatgtatt tatggtgggt
    15241 atagtgtttg gaagtttctt taacgtctgt atttttagga ttccagaagg tatatctatt
    15301 tctagacctc cttctcattg tatgagttgt aatactagac taaagccaat agacttaata
    15361 ccagtgttaa gctatttaat gagtggcaga aagtgtagat attgttatga gaaaatttct
    15421 agtagatatg ctatagtgga attaattaca ggacttttat ttttaacagt atttatgtta
    15481 tatggaatat ctacatcaac tatttattat ttggttcttg tgtctttact gattataatt
    15541 acatttatag atattgacca ctttataata ccagataaaa tattggtttt tggagctata
    15601 ttttctatga tttttaacct tttatttaaa gaaattccag ttaaggattc aattctaggt
    15661 gtttttattt gtggaggaag tgtctggatt atagttcttt tgattgaatt tgtaataaaa
    15721 aaagagtgta tgggtggagg agatatcaaa ctatttgcaa tgctaggttt ttacatgggt
    15781 gttaaaaatg gtcttttgac agccttacta agcgtttatg tgggagcagt ctatggaatc
    15841 tgtgtaataa ttataagtag aataaaagga aaggaatata attctgttat accatatgga
    15901 ccttttatat ctattggagc attaataaca atcttatgtg gaaagcaatt acttgaactt
    15961 tatttttcta tggttatata gatgatagtc aagttaaata aagttttaat ttaaaaaaat
    16021 gaattaaagt atttaataag gacaatatta ttattaaccc tattactaaa gtgctacaaa
    16081 ctatggagta tataataata atttccttat tttttgcaca tttgataaat aaatctctat
    16141 gtgttttttg tgatagagaa aagaaaaatt tatacaatac gttttttatt tacataataa
    16201 ctgtagtttt atctgtatta atatatctaa aatttgggat tagtgtagaa tgtgtacaat
    16261 atatggtttt aattccattt ttaataatta tatccattgt agattactac acaatatatg
    16321 tatatgatat aacaatagtt agtggtataa tagttcaagg tataattttt ttggtctttt
    16381 tcaatatagt tcaatttgat tatataaatc atatatttgg actattgata ggctttatta
    16441 taccttatat tattgctaaa ctaacaaaag gattaggcta tggagatgta ggtctctata
    16501 gcttatgttg ctttgcattg ggaaataatt atgggtttta tgtgatagtt ttatcattta
    16561 ttttagcgtt tttgtttttt attattttat acataactaa aatatttaaa gatgtagata
    16621 taagaaagca aattcctttt acacctttta tttccttgtc aacaatttta ataatattta
    16681 cagagtataa tatatttaat ttttattata atttgttata caaatttata taacaggagg
    16741 taagaaatgt acgtagtagt agggttaggt aatccaggaa agaagtatga gaagactaga
    16801 cataacgttg gatttgatgt tatagatatt cttgctaaag aatacaatat tagtgttact
    16861 aaaataaagc ataaagcttt gattggtgaa gggagagtag gtactgaaaa agtattatta
    16921 gttaaaccac aaacttatat gaatttaagt ggagaaacat taatagatat atataaatat
    16981 tataaggttg atttaagtaa tatagtagta gtatatgatg atatagattt agaagttgga
    17041 aagatacgta taagaaaaaa aggaagtgga ggaactcata atggcatgaa atctataact
    17101 aagtgcctag gttcaaatga ctttccaaga gtgagagtag gtgtatctaa accagaagct
    17161 ggtcaagact tagcagactt tgtactttct agatttagaa aagaagaatc tgataacata
    17221 aatgaagcat tagaaaaagc ggcagatgct attgactcaa taataagaga aaatatagat
    17281 atgtctatga acaaatataa tggatagtga gagggagatt taaatgaatg atgttttttt
    17341 atatcctttg caaaattcaa aagagtataa agatataata aactgtataa aaaatacaaa
    17401 aggctcatta ttggttaatg gacttttacc agtacaaaaa cctcatatat cgtattctat
    17461 atttaatgat ttatcacgac aaatgatttt tataacaagt agtgatttag aagcaaagaa
    17521 agtatacgaa gatttaagtt tttatatgga agataaagtt gaatatttag gttttcaaga
    17581 tatttatttt tatcacttgg atgcaaagga cagaaacgaa gaagcgaaga aattaaaagt
    17641 attgttaaaa ctagcaaata aagaaaaaat cattttagta acttctattg aggcagtttt
    17701 aagaaaatac ataccaaaac aagttttgct cgatagtgta tctcattata aggtgggaga
    17761 tagcttagac ttagaaaagt taactgagaa attagtttca ttgggatatg aaagagtttc
    17821 aaaaatagag gggtttggtc aatttagtat aagaggtgga attattgatg tattttcact
    17881 tgaatataca aatccaattc gtatggaatt atttgatgat gagatagatt ccatacgaac
    17941 ttttgacgta tattcacaaa aatcaattga taaattgcaa caattctcta taactccatc
    18001 tagggaattt atttatcccg aaaaaactac agatgcatta gttaaattaa aaaaagaaac
    18061 aactaaaaat actgatgaag gtacatttca aaatatagat tatatatcaa gtaagactta
    18121 ttttgaaggt gtagagaact atatagatta tatttatcca gaagaaaaca aaagtatatt
    18181 tacttattta gcagatgatg caatagtatt tattaatgat ataacgagac ttaaagaaag
    18241 atgcgaaaat tatataaatg agtttagaga aaattataaa ttaaatcttg aaagaggtct
    18301 ggctattaaa aaccaaggta aattgttata tcactataca gatttagagt acttagtaaa
    18361 agacaagtct gtggtattaa actcgctatt gccaaagtct ataaataact tttctataaa
    18421 atctataata aattttgaaa gtagagaagt tcctacattt aatggaaagg tagatttgct
    18481 tgtagaagaa ttaaatcggc tgaaatataa tggatacaaa ataattttag caacaaatac
    18541 cttagaaaga gcaaataaat taggaaaaga tttattggac aaaggcttag aaacgaccat
    18601 ttctaaagat agagatattg aaattaaatc ttcacaagtt ataattgtac ctgcacacat
    18661 aaacagtggt tttcagtata aatctataaa gtttgttgtt ataacagata acgaaatgat
    18721 aggtgtttat aaaagagcgt ctaagactag caataaaaaa gttaaaaagg gaaagaaaat
    18781 agaatccttt ttagatttaa gtgttggaga ttatgtagtc catgaaaata gtggtgtagg
    18841 tagatacaca ggaattgaac aaattacagt caatgctatt aaaaaagatt atatgaaaat
    18901 agtatatcaa ggtggagata atctatatgt gccaatagac cagatggaca aagtacaaaa
    18961 atatattggt gcagaagtag aaaaagttaa gttaaataag cttggaacaa atgagtggac
    19021 gaaagcaaaa gcaaaagtta aaaaagaaat tgaagatatg acaaaagact tgattgagct
    19081 ttatgcaaaa cgtgaaaaaa tacaagggta taagttttca aaagatacac cttggcaggc
    19141 agagtttgaa tcgttgtttc catatcaaga aacagaagac caattaaagg ctatagaaga
    19201 aactaaaaaa gatatggaat caagtaaggt aatggatagg ctagtatgtg gagatgttgg
    19261 ttatggaaaa acagaggtag ctattagaag tatttttaaa gcttgtatgg accaaaaaca
    19321 agttgctgta ttggttccaa caacaatatt ggcccaacaa cattataata cattcaaaga
    19381 gaggtttgaa aattatcctt tgagagtaga agttttaagt aggtttaaaa caccaaaaca
    19441 acaaaaacag attattgaag atgctaaaaa aggattagta gatattttaa ttggaacaca
    19501 tagaataata tccaaagata taaatttacc aaacttaggt cttgtagtca ttgatgaaga
    19561 acaaagattt ggagtaaaac ataaagaagc tttaaagaaa attaaatcta cagtagatgt
    19621 acttacactt tcagcaactc ctataccaag aacattacat atgtctttaa gtggaataag
    19681 agacatgagt gttatagaag aacctccaca agaaagacat cctgtaataa catatgttac
    19741 agagagtaag gaaagcgtaa ttcaagatga gatagagaga gaattaagta gaggtggaca
    19801 ggtatttttt gtatacaata gagttgaaca cattgaagaa atggcaagta tgattcaaaa
    19861 actcgtacca gatgctagag tggctgttgc acatggaaga atgacttcta aatcattaga
    19921 aaatataatc ctaggatttt taaataaaga ttatgatgta ctagtctgta ctacaataat
    19981 tgaaactggt atggacatat caaatgcaaa tacaatgatt atatatgatg cagataaaat
    20041 gggattggct caactttacc aattgagagg tagggttgga agaagttcaa gacaaggata
    20101 tgcatatctt ttgtatgaaa aagataaaac tttaagtgag atagcggaga agagattaaa
    20161 agctatcaga gaatttacag agtttggttc tggttttaaa atagcaatga gagaccttga
    20221 aataagagga gcaggaaata ttttaggttc tcaacaacat ggtcatatgg cagttatagg
    20281 ttatgatttg tacgttaaaa tgcttaatga tgcaattaaa aaagtcaaag gtgagcctat
    20341 tgtggaggaa attgatgttg aaatagattt aagtgtaaat gcatacattc cagataatta
    20401 tatcaaagat gagttgatta aaattgaaat gtataagaaa atagcttcta ttgaaaacaa
    20461 agaagacatg ttggatatac aagaagaact tgaggataga ttctctgata tacctaaacc
    20521 agtccagaca cttcttacaa tagcttatat aaaatcgtta tgtaagatat taaaaataga
    20581 aaagataaga caattaaaag atgaaatatt attagttcca ataacaaagt atagaactaa
    20641 acagaaaata ggttataata tagttacaga attagaagaa ttattagaaa agatgtgcaa
    20701 ggtaaaataa cttgtaaatt taagaaggga tgttttaaat ttgaaaaaag taataacatt
    20761 ggtaatagct atgattttgg tagtatctgt gactgcttgc agttctagta aaggagaaac
    20821 tgtggcaaca gttgaaggta caaagatatc atcagatgaa tttaaaaaaa ctatagctct
    20881 ttataaagat tctatggaac aaacttatgg aaaagatata tgggataagg aagttgaaaa
    20941 gggtgtaaaa tacaaggaca agtttaaaga tttaattctt gaccaactta taactacaga
    21001 agtaatatat tctcaagcta aaaaagataa cttacttcct aaaaaagagg atgttgaaaa
    21061 aagttttaaa gaattaaaag acgctatggg taaagatgaa aaatataaag aacaacttaa
    21121 gaaattaggt atagatgatg agtttttaag agaccaacaa gagaaagatt tagcaatgca
    21181 aaactatcaa agtaactttg ctaaaaagac taaaatatca gatgaagaaa tgaaaaaata
    21241 ctatgataca cataaagatg aatttaaaaa agatgaggta gaagcttctc atattttatt
    21301 gaaaacagta gatgataata acaagccact ttcagataag gaaaaagctg aagctaagaa
    21361 aaaggctgaa gaggctctta aagaagttaa atcaggtgag gattttgcaa aagtagcaaa
    21421 aaaatattca caagatactt cagcaagtga tggtggtaaa ttaggattct tttcaagggg
    21481 tcaaatggtt gctgaatttg aagatgctgc tttttctatg aaaaagggtg aagtatctga
    21541 tttagttgaa actcaatatg gataccacat aattaaagtg acagacagaa taaatgagca
    21601 aacatcattt gaagatgcta aagaaactat aaaagaccaa ctgttgaaaa ataaatatca
    21661 agaacaaata gaaaaattaa ctaaagaagc taaggtagaa aaagatgaaa aagtaataaa
    21721 taaaatcaca atctaagact taaaaaaaga gtaatatgct taaaaaagct atatctggat
    21781 aagatatagc tttttttgta tatttttctt aacaatggta aaaataaact taaagtaatt
    21841 tatggaggga ttaaaaaata tgaaagcaac aggtatagtt agaagaatag atgatcttgg
    21901 aagggtagtt atcccaaaag agataagaaa gacattaaga attagagaag gagacccttt
    21961 agagatattc acagcaaaag atggagaagt catacttaaa aaatactctc caataggtga
    22021 attaaatgaa ttttcgcaag aatatgctga aacattagct gaagtactag gatatggagt
    22081 gttgataaca gacttagatt ctataatagc tgtgtctaag cttcctaaaa aagattataa
    22141 agaaaaaagt ataagtgatg agttagaagc aataataagt gaaagaacag taaaatattc
    22201 aaaagataaa aacattttac cattattcaa ggaagaccaa aaagaatata ctagtcaagt
    22261 tatcatgcct ataattagtg catctggtga ctgtataggt tcaattgtag ttgtttcaaa
    22321 ggataaagat acaattgcag aaagtgaaga aaaatcttta aaaattgctg ctaacttttt
    22381 aggaaaacaa gttcaataat tttatcataa agacctttta catgaaggtc ttttgaagtt
    22441 tttgtgtaaa atattgaata tagcattaaa aatatggtat attaaagttt gttatataaa
    22501 cagtatggag gttatgttat gaacaataac aaaaataaag actcctttct aaaaggagcc
    22561 cttatacttg ggctagcagg tgtaatcgta aaaataatgg gtggattttt taggattccc
    22621 cttggaaaca tgattggttc agaaggaatg ggatactatc aggctgctta tcctgtgtat
    22681 actctatttt taacattagc tactgctgga tttccaacag ctgtggcaaa attggtttcg
    22741 gaaaaagttg caattggaaa ttttaaaggt gcaaataaga tatttaaggt atcacataca
    22801 gttttattca ttaccggtat aatatcattt tgtatattat tttttggagc cgactacata
    22861 gtaacaaacg taatgaaaaa tccaggtgcg ttatactcta tgaaagccat tgctccagca
    22921 ttactatttg ttcctgcaat gtctgcatat agaggatatt ttcaagggag acaagatatg
    22981 actaagattg ctgtgtctca agttgcagag caatttttta gagttgtatt aggtcttact
    23041 ttggcttact tgcttatgaa gagtctaggt cagacttttg gtgcagcagg tgctatatca
    23101 ggggctacaa taggttcaat agcatctatg ctttatttgg tgtttgctta tatgctaggg
    23161 agaaaagagc gtagagcaga gatagatgca agccaaagat ttaaagatga aagagtgtct
    23221 tatattttta aaaagctttt aactgttgcc attcctataa caataggagc atcagtaatg
    23281 ccacttgtaa atatgataga taatgttatt gtaataagaa gacttatgga agcaggcttt
    23341 acatacaaag tggcaaattc catgtttggt caattaacag gtatggcaat ggccacaata
    23401 aatttaccag cagtcataac aactgcaatg agtatgagct tagttccagc aatttctaaa
    23461 gcatatgcat taggaaataa gtcaaaagct agaaaagata ctaagagtgc agttaaggta
    23521 actttactta tagtattgcc ttgtgcgttt ggtatggctt ctttagcaat accaataatg
    23581 ggtcttttat ttccacatga accatcttct gtaggaacta ttttgtttac tcttactcca
    23641 tgtgtattat ttttaggatt aatacagaca ttgactggta taattcaggg aatgggaaaa
    23701 ccaatagtac ctgtaattgc tttatgtgtt ggtatgctat gtaaaattgt aataagttat
    23761 actttaacag gtataccaga tattaatgta ctgggttcag catttggtac agttacagca
    23821 tactttgtgg cagccatgat taacttacta tatgttaaaa aacacatgaa tgtaaacttt
    23881 tctaaaaaag aatttataat aaagccattt ataacagtta tgacaatgtt tataatggtt
    23941 aagttgagtt atggtgcttt agttggattc ttaggaaaca gcatatctac aataattgca
    24001 atatgtgttg gtggaattgt atatgtagta gtaatacttg gaataggtgg aattaaaaaa
    24061 gaagagattc taactatgcc aaaaggtgat aaattatata aattgttaaa aaaagttaaa
    24121 cttataagat aatataggaa tatatatgca aataaaggaa atactataca aatagtaaat
    24181 ctccttattt gtataataat ataaaaataa agtgaaattt gctaatagga gtgatgttat
    24241 gggtaagata agtattgtag gtcttggacc aggagactat tctcttataa gccaaggtgt
    24301 gttagaatct ttaataaata gccctaaagt tttttttaga acagagaaac atccaataat
    24361 tgataaactt aaagaaacta tacaatacac ttctttagac tacttttatg aaaaagagga
    24421 aaattttgat aatgtttatt tgaaaatatc tgaatttatc atagaagaat cagcaaaggg
    24481 tgacctagtg tatgctgttc ctggtcatcc aagagttgca gagaagactg taagtataat
    24541 agagagatta gccaatgaaa agcacatagc agtagaaata ttgccttcta tgagctttgt
    24601 agacgctatg tttaactatt tggctataga cccatctgag ggatttaaac tggtagatgc
    24661 ttttgaaata gaaaattcat atatagactt ggatacaagt atgataataa ctcaggtata
    24721 tgataaattt atagcatcta atgtaaagtt aaaacttatg gaacattatg actataatca
    24781 agaagtatgt atagtaaatg gagctggtgt caaagattta gaaagtaaaa agtttataaa
    24841 gctttgtgaa ttagacagaa atgaaaactt ttttgactat ttaactagct tatatatacc
    24901 caaaagttct aaaaagatgt ataatacagt acatgactta gaaataatag ttaatacact
    24961 tagaagtcca tctggatgtg aatgggataa aaaacaaaca catcaatcat taaaaaattc
    25021 tgttatagag gaagcatatg aactttgtaa tgcaattgat aataatgata tagatgaaat
    25081 ggtagaagag ttaggtgatg ttcttttaca ggttatattc cattgtcaga taggaaatga
    25141 ggagggctac tttgatttaa aagaagtagt aaatggcata tgtaaaaaat taattcatag
    25201 acatcctcat atatttagtg gtgcaaaatt agatatgtct aagtttgaga aaacttggga
    25261 agaattaaag aaagaagaga aaggcgaaag tactataaca gatggcctta gaaggatacc
    25321 aaaacattta ccagctctta tgaaggctag taaagttcaa cataaagcag cacttgtcgg
    25381 ctttgattgg gacaatgtag aagatgtatt taaaaaaatt gaagaagaat atcaggaatt
    25441 acttgacgaa cacagggtcg ggaatataaa atacataaag gaagagctag gcgatttatt
    25501 attttcgata gtaaatctag ctagattttt agacatagac tcaggagaag ccttgaactg
    25561 tactacagaa aaattcataa atagatttga ctttatagaa aagagtgcaa tgaacttgaa
    25621 taaaaaatta gaagacatga cacttgaaga gatggatgag ttttggaatc aggctaaaaa
    25681 acaataaaat tgtggaagga aatttgtcaa aaatctagaa cttttatcac aaagatagaa
    25741 gtctatatta taaacatttt taggaggtaa atatcgtgaa taaagctgaa ttagtatcaa
    25801 agatggcaga aaaaagtgga ttaacaaaga aggaagcaga agctgcgtta aacgcattta
    25861 tgagttctgt tcaagatgca ctagtaaata atgaaaaagt tcaattagtt ggatttggaa
    25921 catttgagac aagagaaaga gctgctagac aaggaagaaa tccaagagat ccagagcaag
    25981 ttatagatat accagcttct aaagcaccag ttttcaaagc tggaaaagga ttaaaggata
    26041 taataaatgg ataattagaa atcttgtgaa aaagtgtgga taaatttgcc acactttttc
    26101 tatagaacca aggaaggtga taattatgag attagataaa tttttaaaag tatctaggat
    26161 tataaaaaga agaactgttg ctaaagatgc atgtgataaa gggattgtca ctataaatgg
    26221 aaaagttgct aaatcttctt cagaggtgaa tattggagat acattagaaa tacaatttgg
    26281 agagaagaag atgaagttta agataaatga agtaaaagag catgtattaa aaaatgatgc
    26341 aaaagaaatg tacgaaattt tagaataaaa ttaatagtcc tctagtatat taatagtagt
    26401 attattgaaa tatttactgg ggggatatta tggaacagaa cataactttg aaagatagat
    26461 caaagctggt tatttcggga gtcgagcaca tatactcttt taatgacaaa agagtagagc
    26521 tcaaaacttc tgtaggcgag atggtaattg aaggcgaaaa cctagatatg agcaagctaa
    26581 gtatagacga aaatataatt agcatagatg gaactataaa ttctatggta tatgcaaagc
    26641 ctaaaaagcc acaagagagt ttcttgaaaa aggtatttag atagatgata ccttttactc
    26701 aagatgtaag cattttttat gctactattt atggtggaat actcataggg gtgttgtttg
    26761 atttttatag agggctaaga gggaatttta aattcatcaa ttattttgct ataatatttg
    26821 atgtattgtt ttggtttcta gctacggtta taatattcgt aacaataaac cttacagaat
    26881 tttttgattt aagatattat cattttgtgg ctttgtttat tggttttatt ttatactata
    26941 acaccataag taaaattgtt ttatcaatta tcaataaaat aattcgtttt gtgagaaatt
    27001 cttttaaaaa agttacacat tacatagtca gttttttaaa taatttgtat tacgttataa
    27061 tctattcact acacctacta tttgatatta tattttatat accaaacata tttatagcaa
    27121 caagaaaaag tataaaaaga aaatcaaata aaaaattaaa gaacaagaaa aaatcaaaac
    27181 ctaagaagaa aaaaaataaa actaaaaaaa gggtgtagga aaaatgaact taagaaaaaa
    27241 attttcagga cagattattg ttatcagttt atttttagga atatcaattt ttagtatgat
    27301 gacaggattt gtatttgagt atacaaaagc taaggaatat aaaaaagaaa tagcaagtct
    27361 aaataaacaa ttaaaaaaga ctgaaataca aataaacagc ttaaagaaag atgaaaaatc
    27421 atatgaagga gacttagagg atatagcaag aaaaagattg aatatggtaa aaccaaatga
    27481 aacagtttat gtagacataa atcgttgatt aaaacttgct aataagttta cagatgtatt
    27541 gattttaaaa tttaagattt aataaaaaag tcattcagtt aaggatggct ttttgttttt
    27601 attgaggagg gaatttcata atgaaagtag gagctataga tattgggaca aactcaatga
    27661 gattattgat agcagattat agagatggga attttataaa cagaaaaaaa tttgttaata
    27721 caactagaat tggttatggt gtggatagta aaggatatat aaaagaagat gctatcaaaa
    27781 gaaatataaa agcattaaaa gaattttctg ataagtgtaa tgatgaaaag tgtgaaaaaa
    27841 tttattgtat ggggacatct gctcttagag acagtaaaaa tggagattac tttgttaaat
    27901 tagctaaaga agaaactgga atagatgttg atatagttac gggaatagaa gaatcaaatt
    27961 tgggctttaa gggtgtttta gaaggaatac aaggtgatga tactattatt gttatagata
    28021 ttggaggagg ttcaacagaa tttattgtag gtgattcaga aggaattata tttaataaaa
    28081 gtgagaatgt aggtgctctt aggatgacag agaggttttt gaagcacgat attattgaag
    28141 atgaagagtt tgaagctatg agttcattta tatatggtga gattgataaa acgttaaatc
    28201 atataaaaga caaaggatta aaaactctta taggtatagg aggtacgatt acatcattat
    28261 cagcaatgaa tcaagaatta gagatttact ctatggaaaa aatacataat agtaaaatac
    28321 acaaaaaaga aatagaagaa attctacaaa aattaataaa gatgacatta agtgataaaa
    28381 agaacttaaa agggctacaa gaaaaacgtg cggatattat aactgctggt gtaaaaatat
    28441 tgaatataat aatggaaaaa ttagaaatag aagaaataac cgtaagtgaa tacgataatt
    28501 tggaaggctt aatatgtcaa aagtcaaaaa aaatgtatta atattttttg atttttaaaa
    28561 ccgattatga caaaaaaaat tactcctctt tgttacaatt cttaatataa acaaaagagg
    28621 aggttttttt atgcaaagaa gtgtagcagt tgtgaagaat aagactgtaa aaccaaatgt
    28681 cataaaatta ataagcggat atattgattt tactacggcg gcattcgctg tgatgggatt
    28741 tttacttagt aggtctatta tagtagactc tgtagctcct ttaggaattg ccttttttat
    28801 atgtgcatca aagattgata agtacaagat acctgtattc atatcaacat tattaggaat
    28861 attgttatca tctaatgatt ttggaaatgt agcaaaatat gctgtgtgcc ttacaatatt
    28921 tatggctatg aataaaaaat taaaattatc agattcaatt gctaaggttg catttatagg
    28981 tgcaataata ctacttccaa tctccatggg acaagctttc ttttcaaata ggtatgtata
    29041 tggtcttctt atagcaggag tagaagcaat aataatgttt atatctatat atgtgttttc
    29101 ttatgggata ggtttaatgg ccaatgtcaa caataggatg tctgtacgga cagaagaagc
    29161 catatcaata agtttactgc taacctttag cataatggga ataggagata tatctctgtt
    29221 tggaatatca ataaggacca ttttatcgac cgtattgata ttgctgacct caatagtagg
    29281 tggagctact atgggagcat ctagtggtgt aattattggt gtagcatcca ttttaaataa
    29341 tatgacatca gctgtttata tggggatata ttctttttca ggattaataa gtggagcatt
    29401 taataaaata aataaatatt tctgtatact aggatatatt ctaagttgga caataatata
    29461 cctatatact tctggtatta cttctaatat gatgcaatta agagatatac tattaggttg
    29521 tttgatagta ctagtattgc cagagcgatt atttaataag attgaaaaat taataaaaag
    29581 taatgtggct tcaaatgaaa tcgtatacga ttatattatg agaagcaaaa acctaactaa
    29641 tagcagattg aacagtattt ataaaactta tgatgacctt gcagatacat ttgataagat
    29701 aagagaaaaa gacaaagtgc tagaccaaag agacatagct aatgtaatag atatgattca
    29761 taatgatgaa tgtaaaagtt gtagtatgag aagaatgtgc tgggagtcaa gatttaatca
    29821 tacatacact atgatatata acattcttga aaaaattgag gagaaagggg agttaagtct
    29881 aaacgatata cctaaaaatt ttagaaagga atgtatgaaa ccagagtcaa tagtgaaaat
    29941 atcaaatcac tattacaaaa tgtttgtatt agattatgat tggagtgtga agttttcaga
    30001 aagtagaaag ttaatagcaa atcagataag aagtatttca aaatccatta aatccttatc
    30061 tcaagattta gaaggcgaca taatgctaga tatagaaaag gaaaaaaata tatatgaaca
    30121 gttagaaaga tatgatataa cagttgataa agtaagttac ttaacaaaaa gtaacagtga
    30181 gtttgaaata tcaatagaaa agaaaacttg ccatgatgga tgtatgtgtg aagataaaat
    30241 tgtaaatata atttcagacc tagtagggga aaatatgtct gttagaaaaa taggatgtca
    30301 ctgtttagga ggtaaatgca aggcaacatt cgttaaatct caaaaatata aggctgttac
    30361 agaagtttca gcaatgtcaa gggatgggca tatcctttgt ggagacaatt acacatacat
    30421 ggagataaat gatggtaaat acatgatggc tataagtgat ggtatgggaa aaggtaaaaa
    30481 agcatatgaa gaatcttctg ccactattga tatactggaa aaaatgatag atgcaaaaat
    30541 taaagatgag atagtcattg atactataaa taatatgttg ttgttaaagt cttcagaaga
    30601 gatgttttca actctggatt tgggaatttt agatttaaaa cgaggttgtt tagaaactat
    30661 aaagatggga gcgtgttcta cttatataaa aagagaggat ggagaagttg acttaatatc
    30721 gtcatcatca ttacctgtgg gtatattatc agatgtaaaa attgatagaa aaaatgtaaa
    30781 agttaaggaa ggagactatg ttataatggt ttctgatgga attgtagatg caggaagaaa
    30841 taacaattta ggagacaatt ggttaatata cttcttaaag aatatagaga ctacaaatcc
    30901 aaaggaaatt tcaaatttaa tattggatag agcattagag ttacaagcgt tacaaattga
    30961 agatgacatg actgttttag ttacgaaaat atgtacaaaa taaggtttac aagattatgt
    31021 gtataagatg tggataactt gtgtataata tgttaataga cttttgaaaa aagtatagtt
    31081 attaacaaaa ataacatata aatcaacaaa atttgttgat aagtatggca gaaatgttaa
    31141 taaaatgtaa aaaaagaaaa aacaaaagct attatcaaaa gagattcatg ttgaaataac
    31201 taaatttcta agtaaagaaa acctgtgaat atgttgatta ttatgtggat aactattaat
    31261 tcgatattta tttatatagt atgaataatt gaataatata tctaaaaaaa gccatcctat
    31321 tattaagtaa acttattgaa aggatggctt tttatgaata gaaaattgtg caaagcaata
    31381 atacctttat ttatttctat tttttgcatg ttaataataa gtataaaagc tgaccaagga
    31441 tatcaaaaga ataatgatag agcaattact gtcatgaata ataatgatac tgaagatgat
    31501 gctgaaaaaa aacaagaaat agactatgaa aaaacatgtt ggaatttgca atctttgttt
    31561 aaaagtgatg accaatggaa aaaagaactt aagagttttg ataaagatac taaagagttg
    31621 aaaaattaca taggtaaagt aacaaaatcg ccaacacatt tgtcttttgc cttaaacatt
    31681 aaagaaaaac ttgatataag actaaataaa ctttctgcct atgtaaaatt aaaacaagat
    31741 actaataaaa attcgtataa gtacttagat atgaatgata gtatgagtaa atcctatggg
    31801 aattatatgg ggatttgttc agatttagag ttagaaatac taaaattatc aaacaaagat
    31861 tacaagaaaa ttatttcaaa taaaaatata aataggaaat atggtgcata tttaagagat
    31921 ataagaagga ataaagtaca ttatttggat gataaatcag aagatattct aagcaaagta
    31981 tcttcaataa gtagtcttcc aagccaagta tatgaattat ttagaaatat ggatagaaaa
    32041 actaatttga caccagctca atatgcgagt gaattagagt catcagatag agaaaataga
    32101 aaaaaagctt atcaagatga acttataact tacaatgata atataaatac aattgcaggt
    32161 ttaattactg gtcaagttaa aaagaatatt ttttattctc aggaaagagg ttatgagtct
    32221 tctttagaaa tgtacctgga atctgataat gtagatgaaa aagtatacaa taatttgata
    32281 aggactgtaa atagtaatat ggacagtcta cacaaatata tagatttaag aaaaaaagta
    32341 ttaaaattag ataaggttca ttcatatgat atgtttgtgc ctatggttaa ggctgttgat
    32401 aataatatta attatgaaaa ggcacaaagt atagtatatt cagcgttgtc acctttagga
    32461 aaagaataca atgatgtagt atataaagct ttcaatgaaa aatggataga tgtttattca
    32521 catgaaaata aagtaagtgg tggatactgc ttatctgttt atgaaaatca tccatatata
    32581 cttttaaatt ataataattc attaggttca gtttcgactt tatcacatga gcttggacat
    32641 gctgtatatg aatatttaag ttctaagaat cagaattatt ttaattcaaa tccgtctata
    32701 tttactcacg aagttgcatc tacaacgaat gaagcattac tttacgagat gcttataaaa
    32761 gaagctaaag gcaatgatga aaaagcttat tatataacac agtatttaga cttgataaaa
    32821 gatactcttt atacacaaac tatgtatgct gaatttgaaa aaactataca cgagttagta
    32881 gaaaaaggaa aaagtgtaaa cgcattagta ttaaatgatg tttggggaca acttcttaaa
    32941 aaatattatg gacaaagcta tgaattagac caattatcta aggtcggatg ggctagaatc
    33001 ccacattttt ataatagttt ctatgtctat aaatatgcta caggttgtag tgcaggagta
    33061 agttttgccc aagacatcct aaagaatggt tcagataact atatgagctt tcttaaaaag
    33121 ggaagttcag attatccaat aaacttgtta aaatcaagtg gcataaattt aactaataca
    33181 aaacccatag aaaatacaat caaaaaattt gatagtttag tagcagaact tgaaaagcta
    33241 ttataaacat tattgtaatt cacatggtaa atatatcgaa ttaatgataa aatatactaa
    33301 aagtttaaag tcctatcaag ataggacttt ttatttatta attagtggga gatgattcta
    33361 tgcaagttaa agttaaaaag gctgtaatac cagcagcagg tttaggaact agatttttgc
    33421 cagctacaaa ggcacaacca aaagagatgt taccaatagt tgataagcca actctacaat
    33481 acataataga agaagctgtg gcctcaggaa tagaggaaat actaataata acaggtagaa
    33541 ataaaaaatc tattgaagac cactttgata aatctgtaga attggaactt gatttagaga
    33601 aaaaaggtaa gaaagaattg ttggaggtag tacaaaacat atctaacatg attaacatac
    33661 attatataag acaaaaagag ccaaaagggt tgggagatgc aatttattgt gcgaggcatt
    33721 ttataggtga tgagccgttt gcagtaatgt taggggatga tattgtagat aatgatgtac
    33781 cttgtctaaa acaactaaca gatgcatatg aggaatatag aactacaata ttgggtgttc
    33841 aaaaggtaaa tcaagaagat actaataaat atgggataat agaagcaaaa aatatagaag
    33901 gtagagttta caaggtaaaa gacatggtag aaaagccaga gtcaggaaaa gctccttcaa
    33961 atattgctat tttaggtaga tatataataa ctcctgaaat ctttgatatt cttaaggatt
    34021 taccaccagg taaaggtgga gaggtacaac ttacagatgc tttaaaaata ttatctaaga
    34081 aagaagctat gtatgcatac aattttgaag gcaagagata tgatgttggg gacaagctag
    34141 gatttttaga agctactgtt gactttgctc taaagaaaga agaccttaag gaagatttta
    34201 taaaatattt gaaacatgta tgtagtgaat ttgataaaaa taacaatgtg ttatacaata
    34261 atacagatga aaaaatagat ttaattgaag tcaatgaagc tagagaactt gaaattcaaa
    34321 aataattaat gaaaggtgat gtatggtggc aaaatcaagt cagaaaagaa ttaatagaat
    34381 aatagctgtt gggattgtgg gaatcctcgt aataacgagt ataacagctg ttttgagtat
    34441 gattgcaact atgtaataag ataaatgaca gcagttgata tacataaata taaaaaccat
    34501 atataacttg atgtagtcaa aaacttacaa tattttagtt atatatggtt tttagaattt
    34561 ataaatgtaa aaaaaaattt ttataatagt tgcatatttt aaaggaaaat attattataa
    34621 gatataaata tagtgataaa tttataaaaa cgtgctaaga ttggacttga aatacagtaa
    34681 tatagtaggt aataatgttt taaattttat ttattgacca taatatataa ataatgatat
    34741 tattagactt aactgtttaa ataaaacttt tctttttcat gccttcaatt tccaaaataa
    34801 gtatgtatat aaatgaatat atagatgagg aggatttttt tgattttaga tgataaagta
    34861 ttagaaggat taggtagtaa gaccttagtt gagctacgag agatagctaa agaattaaaa
    34921 ataaaatcaa ttactacata taagaaaaat gagttaattg agattataaa ttccaaaagt
    34981 aaaaatgagg aaaaagtaaa cgaagagact aaagaaaaaa atgaagatat taaagaagac
    35041 ataaagaata gaaaagaaaa tatagagcgt gaaagtatag aaaaagaagt tgaatataag
    35101 agccctacca aatcatacaa caagaaagaa atagataccc aaaatatttc ccaaaaccaa
    35161 gaacatagaa atcaaagaaa tgactataat aagacaacga gtgtaaatga tagtaataag
    35221 tcatcaaata tatctaatag aatggtcaga aataataata aaaactatta tatgcctaaa
    35281 caagttgatg aatctaagat agtagatgaa tttaacacat caaaagagga tgaagtagta
    35341 ggtgtacttg aaattctgcc agatggattt ggttttttaa gaggttcaaa ttatttgtct
    35401 actgaaggag atgtttacgt ttcaccatct caaataagaa gatttaatat gaaaacagga
    35461 gacaagatta agggaataac tagacatcca aaaagtggag agaaatttag agcactttta
    35521 tatgttcaaa aaataaatga tgaaaaccct gacacagcta tacaaagaaa tgcgtttgag
    35581 acattaacac caatatttcc agaagaacga cttacactgg aaactaatag aaatgaaata
    35641 gctacaagaa ttatagattt aatttcacct ataggaaaag gacaaagagg attgatagta
    35701 gcacctccaa aagctgggaa aacagttctt ttaaaaagcg ttgcaaatag catagctaaa
    35761 aatcatccaa atgttgaact tatagtactc cttatagatg aaagaccaga ggaagttact
    35821 gatatgaaag aatctattga aggagacgtt atatattcca cttttgacca agtatcaagt
    35881 catcatgtca aggttgcaga gatggtgtta aatagagctc aaagattagt tgaacatggt
    35941 aaagatgtag taattttact agatagtata accagattgg caagggcata taaccttaca
    36001 atatctccta caggtagaac tttatctgga ggtattgacc ctggtgcact tcatggacct
    36061 aaaaagttct ttggtgcagc aaggaatatt agacagggtg gttctttgac aatattagga
    36121 acagcattgg ttgaaactgg ttctaggatg gatgatgtaa tatttgagga atttaaaggt
    36181 acaggaaata tggagctaca tttagataga aaattagcag aaaaaagaat attccctgca
    36241 atagatattt acaagtctgg tacaagaaga gatgatttat tgcttgatga tgaagaaaag
    36301 actgctcttt ggagattaag aagagaaatg agtaataatt cagtaatgga aattacagat
    36361 aaagtcattg aacttataaa gagaactaag gacaataaag agtttgtaaa atcaataaag
    36421 aatttgtaaa atcaataata caaatgcaaa tatggattat ataaactaat agaaaaatca
    36481 cttgaatggc ataataagtt gtgctataat aatgtagttg ataatgtaaa aagaaagtta
    36541 atataattaa tttttttaaa taaagagaag aggtgactat aatgcaaaag gaaatacaac
    36601 caaaatacaa tccagttgaa gtgcgttgtg cttgtgggaa cacatttgtt gctggttcaa
    36661 ctaaggacga aataaaagtt gaaatatgtt cagagtgcca tccattctat acaggaaaac
    36721 aaaagaacat agaaaaaggt ggaagaatcg acaaattcaa gaaaagattc aaaatggact
    36781 aatttttata aaagtggagt tggaggaagc caactcctat tttattacaa aaaaacattc
    36841 atattctact ttaaattgtc tttttgtatt ataataataa atatgtaaaa ttgagtattc
    36901 taaatacaat aaaatgaggt gagtggatgg caaaacaagc tgtaggtggg caagctgtta
    36961 ttgaaggtgt aatgatgcag tcaaaagaca aaagagcggt agctgttaga aaaagtgacg
    37021 gagaaatagc actaaaagaa gatagaataa aaagttgggt aagagataag aatatagata
    37081 agataccttt tgttagaggt tcttttgtta tgatagatac aatgattcaa ggcataaaaa
    37141 gtttaaactt ttcttcagag ttttttatgg aagaagctga agaggataaa tttgatttat
    37201 ttataaagaa aatttttaaa gataaggcaa atgatataat tataatattt tcattggtca
    37261 tagcaatgtt actttctgct ggattattca tttttatacc tacattggta ggaggagctt
    37321 tttctaaagt aatgcctaat gattttatgc ttaatttaat agaaggaatc ataaggatag
    37381 ctatattatt tgcatatata gtattgattt cacgaagtaa ggatatagaa agagtctttc
    37441 aatatcatgg agcagaacat aaatctatat attgctatga aaatgattta gaacttacag
    37501 tagagaatgc tagaaaattt aaaagattac atcctagatg tggtacaaat ttcctattta
    37561 tagttatggc tgtatcaata atattgtttg cattttttgg ttggccaaat ccaattctta
    37621 gaatatttat gagaattata tgtgtgccta tagtagcagg tttatcttat gaggtaatta
    37681 gagttcttgg gaaatatgat aatggattta ctaaaattat tgcatatcca ggaatgatgt
    37741 tgcaatattt tacaacaaaa gagcctgatg atgagcaatt agaggttgct ttggaagcat
    37801 tgaaagcagt agtagattag agaaaaggat atttgttatg acgataaaag atataattat
    37861 taaatattca gataaactaa aagatataag tgatactcca agattagata cagagttatt
    37921 gttacaaaaa acattgggtg ttgatagatt atatattcac ttaaatctga ataaagagct
    37981 aactgaagaa caaaaaacaa aatttatggg ttttgcagaa gaaagattaa atggaagacc
    38041 aatagcatat atagttgaaa atagggagtt tatgggatta gatttttttg ttaaggaagg
    38101 cgtattgatt ccaagaccag atacagaaac tttagtagaa gaaataattg aaatatgtag
    38161 agaaaaaaaa gatgtaagta tattagatat aggaactggt tctggagcta ttacaataag
    38221 tttagcaaag tacattgaaa attctaaaat catgtctttt gatatatcag agacagcatt
    38281 agaaatagct aaaaaaaatg ctattataaa tgaagttggt gaaaaaataa agtatattaa
    38341 ttctgattta tttacagcaa taagtgatag taacataaaa ttcgatatca tagtttcaaa
    38401 tccaccatat attaaaaaac aagatataga aactctgcac aaacaagtta aagattatga
    38461 accatataat gctcttgaag gtggagaaga tggattagac ttttatagaa gaataacaga
    38521 acaaggtaag aaatatttaa ataagtgtgg aatattagca tatgaagttg gtcataatca
    38581 agctgaagat gtaataaata ttatgaaaag taatggatat aaaaaaatat acacaaagaa
    38641 ggatattcaa ggcattgata gagttgttat aggttacaat atatgatata atcaacaatt
    38701 gatatgttac atttaatcga ggtgaaagta tgttaaaaaa attagaggta ttggaagata
    38761 catataaaga cttaagtgaa aaaataggtg atccggatgt aataaatgat caaaaagttt
    38821 ggcagaaata tataaaagaa catgctgact tggaacctat tgttatgaaa tacagagagt
    38881 ataaaagcgt tttagatagt ataaaagaat caaaagaaat tttacaagaa gagtcagatg
    38941 aagaattaag agaattagct aaaatggagt tagctgagat ggaagaaaaa gtagcacctc
    39001 tggaggaaga aataaaaata ctattattgc ctaaagaccc taatgatgat aagaacgtta
    39061 tagttgaaat tagaggtgga gctggaggag atgaagcagc tctatttgct ggtgatttat
    39121 ttagaatgta ctcaaggtat gctgaaagaa gaagatggaa aatagaatta ttgagtgcaa
    39181 gtgatactgg agttggtgga tataaagaag tatcttttat gataaaaggt aaaggtgctt
    39241 attcaagatt aaagtatgaa tctggagttc atagagttca aagaatacca tcaactgaat
    39301 ctggtggaag aatacacaca tcaacttcta ctgttgcagt attaccagaa gttgaagatg
    39361 tagaggttga aataaatcca aatgatttaa gaatagatgt tttccgttct tcaggtaatg
    39421 gaggacaaag tgtcaatact actgactctg ctgttagggt aacacatata cctacaggtg
    39481 aagtagtatc ttgtcaagat ggaaaatcac aattaaaaaa taaagagcaa gctttaaaaa
    39541 tattaaaagc tagactttac gataaagcat tggcagaaca acacaaagat atagcagcag
    39601 aaagaaaaag ccaagtcgga acaggagaca ggtcagaaag aataagaaca tataatttcc
    39661 ctcaaggtag aataagtgac catagaataa atttaacttt atataagtta gatgcattct
    39721 tagatggaga tatagatgaa atgatagatg ctttaattac tgttgaccaa acagaaaaaa
    39781 tgactgcaat ataagtaatg ttttaaagaa gctgtttctt cctagaaatt attttctatt
    39841 ttgaaacagc tattttgcat tttaggtcat gttatcatat tctattgtat ataattgtta
    39901 tatacaagta taggagggtt atatgatact aaaagtaaca ataattgggc ttttggcagg
    39961 tgttataggg acaggattag gaggagtaat atcagccatt tttaaaagag aggtcgataa
    40021 atatctaagt ttttttatgg gattatctgg tggaataatg ttggctgtag tggtgtttga
    40081 tttaatgaaa gaatccatgg acaaaatggg gataatcaat acagttatat ttacgtttgt
    40141 tggagctctc ataactatgt acataaaaac aaaattagat gtgtctggaa atatggcatc
    40201 tggatatctt atattcataa gtatattact acataattta ccagaaggac tagccattgg
    40261 ttcttctttt atgtcaactg aaagtttagg tattacactt gctatagtta taggtctaca
    40321 taatatacca gaagggttag ctatggcatt aggcttagtt tgcaataaaa tgaaactaag
    40381 taaagttatt ttgtttactg taatagctgg attacctatg ggtctaggaa gttttttagg
    40441 tgtttatttt ggaggagtat ttacttctct tataggagta tttcttgcta cagcaggagg
    40501 tactatgatg tatgttgtat tggaagaaat ttttccacat tcaaaaagtg tgtattgtat
    40561 aattggtttc ttgttaggta caatgattgt aaattatata taaaattaaa atgaaagcag
    40621 gcatacaaaa gatgaaaact attataagta atattgatat aaataatatt gattatgagg
    40681 aaataaaaat acaagcaaaa cttcttagag aaggtaaaac ggttattttt ccaactgaga
    40741 cagtatatgg gttgggtgca aatgcattag atgaaaatgc agtaaagaag atttatgaag
    40801 ctaaaggaag acctagtgat aatccattga tagtccatat atatgaaaaa gaagaagtat
    40861 atgacttagc caaagatata agtgataaag ctaaacttgt tatagaaaag ctttggccag
    40921 gacctataac aattatatta aataaaaaag atataattcc atacaaaact agtggagggc
    40981 ttgatacagt tgcaataagg atgccttcta atgttattgc cagggcaata ataaaagaag
    41041 ctggtatacc tattgcagct ccttctgcaa atatctcagg tcgcccatct ccaacaaaag
    41101 caaaacatgt atatgaagag atgaatggaa gagtggatgg aatagtttta ggaggagaca
    41161 gtaattttgg attggagtca actgtattgg atttaacaga agagactcct atgatactga
    41221 gaccaggaag tataactaaa gaagttttag aggaacttct tggagaagta aagcttgacc
    41281 catcattaag taaaaaagaa gataatcaaa aagctaaagc tcctggaatg aaatataaac
    41341 attattctcc aaatgcagat gtatatataa tatctggaaa aagagaaaat gtagcaacaa
    41401 agattaatga tatgataaaa tctaatagag acaaaggtct aaagacaggt gtaatgtgta
    41461 ttagtagaaa ccgagagttt tatgatggag aagtaatcga tttaggaaac tctcttgaag
    41521 aagttgcaag taatctgttt gatgctctta tagaaatgga caaaaagggt gttgatgtca
    41581 tatattcaga agagttcccc aagactggag ttggacaagc aattatgaat agactactga
    41641 agtcagcagg atacaaagtg ataaaggctt aagagggagt tgtaaaatat gaatatactt
    41701 attgtgtgta caggtaatac ttgtagaagc cctatggctg aggctatact aaggaaagca
    41761 attaaagaat ctggaagaag tattgaagaa tattctatat catcagcagg tatatctaca
    41821 gcaaatggaa tgggtgcttc tgaaaattct atagaagtac taaaagaaat cggtatagac
    41881 ttatcaaatc atagaagtaa agtaattact aaaaaattaa ttgatgaatc agatataata
    41941 ctaacaatga ctaaatctca taaagaaatt ttagtacaag cagttccaaa atgcaaagaa
    42001 aaagtgtata cttttaaagg gtttgcaaat aaaaatgagg aagatatatc tgacccattt
    42061 ggtggaaatt tggatattta tagaagcact atgagagaaa taatgtattc tgtcaacgaa
    42121 atagtaaaaa aaatatgaaa cggaggataa acaaatgaag ataggattag gctgtgacca
    42181 tggaggatat aatttaaaaa aagaaataat aagctattta gaaagtaaag gtatagagtg
    42241 tgtagattat ggaactaata atgctacaga ttctgttgat tatcctgttt atggagaaat
    42301 agttgcaaat tcagttataa ataaagaagt agattatgga atattatgtt gtggtacagg
    42361 gataggaata tctttagctg ctaataaggt tcctgggata agatgtgcag ttgtatcaga
    42421 tgtattttct gcaaaaatgt caaaagcaca taatgatgca aatatgttat cattaggcga
    42481 aagagtttta ggaaaaggtc tagcacttga gatagttgaa gcatggatta atacggattt
    42541 tgaaggtgat agacatgcta gaagagtaaa catgattaaa tcaatagaag aaaaacataa
    42601 taaataataa aaattacagt tttatcagga ggaattatca aaatgagcaa agtggtagag
    42661 acaaaccatc cattaataca acataaatta actttaatga gagataaaaa tactggttct
    42721 aaagacttta gagagctttt aactgaaatt gcaatgttaa tggggtatga aataacaaaa
    42781 gatataccat taaaagatgt agaaatagaa actcctattc aaaaaacatc atctaaagta
    42841 gttgcaggta aaaaattagc aataattcca attttaagag caggtcttgg aatggtagat
    42901 ggattagtga gcttaatgcc agcagcaaaa gttggacatg taggactata tagagaccca
    42961 gaaacattaa aaccagttga atattattgt aagcttcctc aagacatagg agaaagagat
    43021 ataatagtag ttgaccctat gcttgcaaca gggggttctg ctgtagcagc tatagattta
    43081 cttaagtcta aaggagctaa aagtataaaa ttagctaatt tagtagctgc accagaaggt
    43141 atagcagaag tgcaaaaata tcatgatgat gttgatatat atgttgcatc agttgacgaa
    43201 agattaaatg aacatggata tataattcct ggtcttggtg atgctggaga tagattgttt
    43261 ggaactaaat aaaaataaat aactataaat at
//
LOCUS       DAYAOX010000008        42030 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.008, whole genome shotgun sequence.
ACCESSION   DAYAOX010000008 DAYAOX010000000
VERSION     DAYAOX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.008"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(80..301)
                     /locus_tag="ACGI57_000349"
     CDS             complement(80..301)
                     /locus_tag="ACGI57_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFG9715439.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(385..1611)
                     /locus_tag="ACGI57_000350"
     CDS             complement(385..1611)
                     /locus_tag="ACGI57_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFG9715440.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(1673..2605)
                     /locus_tag="ACGI57_000351"
     CDS             complement(1673..2605)
                     /locus_tag="ACGI57_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFG9715441.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            complement(2694..3221)
                     /gene="hpt"
                     /locus_tag="ACGI57_000352"
     CDS             complement(2694..3221)
                     /gene="hpt"
                     /locus_tag="ACGI57_000352"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFG9715442.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            3450..4526
                     /locus_tag="ACGI57_000353"
     CDS             3450..4526
                     /locus_tag="ACGI57_000353"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFG9715443.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            complement(4612..5820)
                     /locus_tag="ACGI57_000354"
     CDS             complement(4612..5820)
                     /locus_tag="ACGI57_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFG9715444.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(5981..6370)
                     /locus_tag="ACGI57_000355"
     CDS             complement(5981..6370)
                     /locus_tag="ACGI57_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HFG9715445.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            complement(6474..7475)
                     /gene="asnA"
                     /locus_tag="ACGI57_000356"
     CDS             complement(6474..7475)
                     /gene="asnA"
                     /locus_tag="ACGI57_000356"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFG9715446.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     misc_binding    complement(7534..7789)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7923..9113)
                     /locus_tag="ACGI57_000357"
     CDS             complement(7923..9113)
                     /locus_tag="ACGI57_000357"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9715447.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEISRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     gene            complement(9394..10515)
                     /locus_tag="ACGI57_000358"
     CDS             complement(9394..10515)
                     /locus_tag="ACGI57_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HFG9715448.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            complement(10576..11061)
                     /locus_tag="ACGI57_000359"
     CDS             complement(10576..11061)
                     /locus_tag="ACGI57_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFG9715449.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            complement(11063..11731)
                     /locus_tag="ACGI57_000360"
     CDS             complement(11063..11731)
                     /locus_tag="ACGI57_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFG9715450.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAVLFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            12121..14145
                     /locus_tag="ACGI57_000361"
     CDS             12121..14145
                     /locus_tag="ACGI57_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9715451.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            complement(14375..15562)
                     /locus_tag="ACGI57_000362"
     CDS             complement(14375..15562)
                     /locus_tag="ACGI57_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HFG9715452.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(15764..17035)
                     /gene="brnQ"
                     /locus_tag="ACGI57_000363"
     CDS             complement(15764..17035)
                     /gene="brnQ"
                     /locus_tag="ACGI57_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFG9715453.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            complement(17176..17829)
                     /locus_tag="ACGI57_000364"
     CDS             complement(17176..17829)
                     /locus_tag="ACGI57_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFG9715454.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     regulatory      18158..18259
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18332..19675
                     /locus_tag="ACGI57_000365"
     CDS             18332..19675
                     /locus_tag="ACGI57_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFG9715455.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            19854..21218
                     /locus_tag="ACGI57_000366"
     CDS             19854..21218
                     /locus_tag="ACGI57_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFG9715456.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(21396..23261)
                     /locus_tag="ACGI57_000367"
     CDS             complement(21396..23261)
                     /locus_tag="ACGI57_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HFG9715457.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(23497..24714)
                     /locus_tag="ACGI57_000368"
     CDS             complement(23497..24714)
                     /locus_tag="ACGI57_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9715458.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(24914..25777)
                     /gene="aroE"
                     /locus_tag="ACGI57_000369"
     CDS             complement(24914..25777)
                     /gene="aroE"
                     /locus_tag="ACGI57_000369"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFG9715459.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(25859..27781)
                     /locus_tag="ACGI57_000370"
     CDS             complement(25859..27781)
                     /locus_tag="ACGI57_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9715460.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(27845..28693)
                     /locus_tag="ACGI57_000371"
     CDS             complement(27845..28693)
                     /locus_tag="ACGI57_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFG9715461.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            29106..29987
                     /locus_tag="ACGI57_000372"
     CDS             29106..29987
                     /locus_tag="ACGI57_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9715462.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(30195..31034)
                     /locus_tag="ACGI57_000373"
     CDS             complement(30195..31034)
                     /locus_tag="ACGI57_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HFG9715463.1"
                     /translation="MSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEYKYAVSQGD
                     YVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDELPLFLSRL
                     ECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARIVIESRDDV
                     SNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIADILCNPLS
                     RARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIVEPVGPWLE
                     KMR"
     gene            complement(31213..33147)
                     /locus_tag="ACGI57_000374"
     CDS             complement(31213..33147)
                     /locus_tag="ACGI57_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HFG9715464.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(33259..34272)
                     /gene="galE"
                     /locus_tag="ACGI57_000375"
     CDS             complement(33259..34272)
                     /gene="galE"
                     /locus_tag="ACGI57_000375"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFG9715465.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(34296..35171)
                     /gene="galU"
                     /locus_tag="ACGI57_000376"
     CDS             complement(34296..35171)
                     /gene="galU"
                     /locus_tag="ACGI57_000376"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFG9715466.1"
                     /translation="MQMTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIE
                     EAIESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHY
                     IRQKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGV
                     QKIAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSIL
                     EKQTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENL
                     KDDLMDYMRKKVSEK"
     gene            complement(35363..35683)
                     /locus_tag="ACGI57_000377"
     CDS             complement(35363..35683)
                     /locus_tag="ACGI57_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715467.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(35673..37664)
                     /locus_tag="ACGI57_000378"
     CDS             complement(35673..37664)
                     /locus_tag="ACGI57_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715468.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(37787..40330)
                     /locus_tag="ACGI57_000379"
     CDS             complement(37787..40330)
                     /locus_tag="ACGI57_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFG9715469.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(40608..40942)
                     /gene="rnpB"
                     /locus_tag="ACGI57_000380"
     ncRNA           complement(40608..40942)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACGI57_000380"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            41074..41751
                     /gene="srtB"
                     /locus_tag="ACGI57_000381"
     CDS             41074..41751
                     /gene="srtB"
                     /locus_tag="ACGI57_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HFG9715470.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(41911..41985)
                     /locus_tag="ACGI57_000382"
     tRNA            complement(41911..41985)
                     /locus_tag="ACGI57_000382"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41951..41953),aa:Thr,seq:tgt)
BASE COUNT        13829 a         7263 c         4679 g        16259 t
ORIGIN      
        1 tcaatttaga aattactttc tatatttgag atagctctag ctaaactaat agccaatagt
       61 tataaataat tatttataac tatctgttgc tttttccagc catttgttgt tcagccattt
      121 caactaattt tttagtcatg tatccaccta cataaccatt ttctcttgca gtaaggtttc
      181 ctttatcaac ttgttgataa ttactcatac caagttcatt agctatttct aatttcattt
      241 ggtttaaagc ctcttttgct cctggtacta ctgttctgtt gttgttattg ttacttgcca
      301 tgttgattac cttccttctg tttaatttat tttgtgttgc tgataatatt atgtgatttt
      361 aaacattttt aattcaggta atttttagca attaaaacct gccaaatatc cagtagaaaa
      421 agctatttgc aaattgaatc cacctgtata tgcatctata tctattactt ctccagcaaa
      481 aaataaacct tctactaatt ttgattccat tgtacttgaa tttatttcgt ttacttttac
      541 accaccagaa gttattatag cttcttcaat aggtctgtat ctctttactg taaactttaa
      601 attttttaat aaatggacta agttttttct ctgctctcta gaaatttggt gaacaaccgt
      661 atttggatta atttcactta agtttattat tataggtatt agtttttttg gaagtaaatc
      721 atctaatgct ttttcaaatt ttttatttgt atacttctga aaatcttttt gaactctttt
      781 atccaatttt tcctcatcta atgcaggttt taaatctaat aatatagtgt aattttcttt
      841 ccttgtatct ttcattctac aacttgcaga cttgattata ggtccatcta aaccaaattt
      901 agtaaattca agttctccaa aatcactata aactttcttt tgtttgctat taaacaccct
      961 tatttcaaca tttctcaatg ataatttttc taaatctttt gtaaaacttt cttgtacttc
     1021 tataccaatt aaagatggtt ttgtatctat aattgtgtgc ccctgtgaaa ttgcaaattt
     1081 atatccatca ccagtagaac cagtaagtgg ataacttaat ccaccagttg ctactacaac
     1141 tgaatcacac ttaatttctt ttttatcgtt taaaattact ttttcaattt tattattttt
     1201 agatattatt ttttctactt tcgaatttaa taatatattt actttcttac ttttaagttg
     1261 tctttcaaga gcatttacaa tatcaaatgc tttatcactc tctggaaaaa ccctttttcc
     1321 tctttctgtt ttagtcttta ctccaagatt attaaacata cttattacat catcatttgt
     1381 aaaagtataa aaagcactat aaagaaattt tccatttgta ggtacatttt ctattaattc
     1441 ctctatttca caagcgttag taatattaca tctacctttt cctgttattg caagtttttt
     1501 tcctaattta tgatttttct caattaatgt tacatcatag cctttctcac atgcagttga
     1561 tgcggctatc atccctgctg gtccaccacc tattattact accttcttca aataatcact
     1621 cttttctacc cttaattttt ttaatttaaa ctattgactc tataccttaa aatcaattta
     1681 attttaaaag tttaaataat gctggagctc ctgctattcc ataaaaaaca actactgtat
     1741 tcttaataaa attcataaaa attatactca tagtagagtc acttatattt aaagtgacta
     1801 ttaacattga aataaacaac ttaatgccaa tatataacat tcctattgtt actatcccaa
     1861 ccaaaaacct acatattctt gaaaaaatta tatttttact ccccttattt ttattgctaa
     1921 aatttatttt tttgttatta ttatctgtat taaatttaac aaatctatcc tcaaccatat
     1981 atccaaatac aaatcctact attataccaa atttttcaaa atattcaggg ctatttagaa
     2041 atatagccac tattataaat ggtataagta aaactacaag taaaatataa tttttattat
     2101 catcaatata gtcaaatatt tttacaaata caactgtaaa aaatattcct aatatccaac
     2161 ccgctatcac atctattggc caatgaacag ctaaatacag cctagacaat cctactccta
     2221 aaaccataac tgcacctatt aaatgaagat atctcttttt aaatatagtc ataatactaa
     2281 cccaaaatgt tgttgcagtt tgagtatgag cacttggaaa agaataacca ccagcagtag
     2341 aaactctcaa tgattcaaga ccttttattc ctattggtct gggagctttt acaaactctt
     2401 tgatgcctaa atttacaaca aaatttccaa ttatagcaaa cagtattttc tgtccacatt
     2461 ttttatttat acaccagtat ataattgttg taattaatat taataacggt gcttctgtgc
     2521 ttattgtaaa gacaagaaat ataaagttca aaattggatt tcttaaattt tggaaaaata
     2581 ataatatatt taactgaata tccatataga tgtctccttt actaaaattt tatataatta
     2641 actaaaaagc actaactccc ttagaaatta gtgcttttga ttcacattta aatctattct
     2701 acatcctcaa ctatacctat ataaggtaaa tttctatact tttcagcata gtctattcca
     2761 taaccaacaa taaatttgtc ttctataaca aatccaacat agtctacagg tatattagct
     2821 tttcttctct gaggtttatc aagtagagta catagtttta aagattttgg atttctagtt
     2881 tttaaagctg caactagatt acttaatgtt aaaccagaat ctattatatc ctctacaatc
     2941 aacacattct ttccttcaat atctacatct aaatctttta atattttaac tgttccagaa
     3001 gactctgttg agtttccata acttgtaaca ctcataaaat ctatatttac atccaaatct
     3061 atacatctta ttaaatctga aacaaataca ctagctcctt taagtatacc taccactagt
     3121 aaatcttcgc ctttaaagtc ttcttctatc ttttttccta attcataaac tttttctttt
     3181 atttgttctt ctgtcaacat ttttcctgta actttgtaca ttttgtatcc tcccgctttc
     3241 cttctattaa cttatatatt atagcatacc aaaatatcaa ttaaaatctt tttcatcatt
     3301 tttaaaccta aattaacagc ttatccatac ttttttgctt tttacattaa aaatttggaa
     3361 taacgattcc tttgtattat aatctatatg tacatatatt aatcagttat ttttatcgat
     3421 tatataattt tatttttgga ggtactttta tgagcacaaa ccatggagct aatttatata
     3481 gtttatctag taagtatggc ttttcaaagg aagaatttat ggattttagc tcaaatataa
     3541 atccttttgg tacatcaagt ttagccaaac aatacattgt aaacaatata gatatggtat
     3601 ctatgtatcc tgaccctgat tacatagact taaaaacttc tatatccaat tactgcaaat
     3661 gttctataga taatatagta cttggcagtg gagctacaga actaatttca tcatttatac
     3721 atacaattaa ccctaaacag gctcttttac tctctccagc ctattcagag tatgaaaaag
     3781 agttatctaa aattaattgt tctatagaaa aatactttgc aaaagaagaa gataattttc
     3841 atataaattt agaaaatctt ataaaaacta tcaatgctaa agattatgat ttggtagtaa
     3901 tctgtaatcc tagtaatcca actggatttg catttactaa agtagaagtg agagaaattt
     3961 taaaaaatac agactcattt ttaatgattg atgagactta tgtagagttt actgatacag
     4021 atacatactc ttgtactcaa ttagttgatg attattctaa tttattcgtt ataagaggta
     4081 catcaaaatt tttttcaacg cctggaataa ggcttggata tggtcttata tctaacacta
     4141 atgtcaaaaa tgaaataaat aaaaatctag atttatggaa tataaacata atagcttcta
     4201 aaatgggtga aatcatgttt agtgatttag attttatatc aaatactatt tccttaatga
     4261 acactgaaag agattacctg cttaaagagc ttaagaatat aaaaagctta gatatataca
     4321 atactaaagg taatttcata ttgtgtaaaa tcaagacaaa ggaattgact gctaaatctc
     4381 ttcgagagca actgcttcca caaaaaataa taataagaga ttgttgttca tttgaaggtc
     4441 tagatgagta tttctttaga gtatgtatat taaaacctaa tgaaaataaa ttattaatat
     4501 catctcttaa agcaattttt aaataaaaaa atcactttat tttataattt tttataccat
     4561 aaaaaatgag ctatctctat ttgagataac tcatttttga ttattaaaaa actaagcact
     4621 ttgctcttct ttatttcctt ttttactcat tccatcattt attttatcta ctactactgc
     4681 tattgcatta tctcctgata cattacatgc tgtaccaaaa ctgtcttgag ttaagtataa
     4741 agctatcatt aaacttgcaa gcccaccatc tgatggtatt ccaaccattg gtaggaaagg
     4801 aagtgcactc attatagccc cacctggtgc tcctggtgct gcaaccattg ctatacctaa
     4861 cattgcaata taagggaata atacacctat tccatgtggc ataccattca tcattaaaac
     4921 tgcagttgta aatcctgtta ttgatatcat actacctgat aaatgtattg ttgcacaaag
     4981 aggtacaaca aattctctta tttgcttact tactccattt ttttctgcac aaccaacatt
     5041 aactggtata gttgctgctg aagattgagt tcctatagct gttaaatatc caggtacttg
     5101 attttttaac attcttactg gattttttcc tgaaaataca cctgctgctg tgaattgaca
     5161 tgccatatat acaaaatgta aaggtattac tactaagtat actttccaga atacccctaa
     5221 tattgtccat acttcccctg ctatagttat atttgcaaaa gttcctgcta tgtataaagg
     5281 taataaaggt ataatcactg ttgacaacgt tttcgttacg attccttgaa attcttggaa
     5341 gaaattaaat aaagtctctc cttttccatg atttcttaaa gctgatattc caagtcccat
     5401 tacaaatgca aatacgattg cagctgttac atctaccata ggctctaatg gaattgtgaa
     5461 tattggagct aacattcctg cctctgggtc tccaatttga cttgcaacac ttgcatcaat
     5521 aaaatttggg aagattaatg tagatactgc aaatgataat aatcctgcta ttaaggtaga
     5581 accatatgct attcccgtag ttatccccaa taactttcct gcacctgttg ctaaatcagc
     5641 tatacctgct gttacaaaac ctataattat aaatggtata acaaatttta ggaatgctga
     5701 gaataaagta ctaactgtta ctaatatttg tactattatt tctggtgcat aacttccgat
     5761 taaaataccg actatgatac cgatgattag tttgggcact aaacctattt ttttcttcat
     5821 aatatttacc ctccataact tttttaaatt aagcattctt gcacattgaa tacttagtta
     5881 aatattggat atccacttat tatgataata gagttttacc tctccgtcaa caatttaacc
     5941 attttttgtt aataaaaaaa agaataacat atataaattg ttattcttct tcatctttta
     6001 tagaaaattc tacattttca attatatttg aatgtttatc tttttttgcg ataactctat
     6061 acttattaat ttcttcattc actataccta tactccaaat taaaactata gcatcatcta
     6121 taaaaccaaa tgcccctgca atcatctctg gaattatatc aaccccagat acaaagtagg
     6181 atatagtaaa aattaataca gaaattacct ttattttatc aactatattg attcttttat
     6241 ctgtcataaa atcaggtagt ttaagtaaat caaatacaaa aaccaaacca aatttagcat
     6301 ttttaaatct gaccaaaatt ttttttaaaa tatctaaaac tatattaatg attattttta
     6361 aaaactccat atttatcacc tcttataatt ataggttatt ctacattata accgatataa
     6421 ttaatttgat gaacatatta gatataaatt ttatcaatta gtttatattt aaattataat
     6481 aattcaattc ctgcactact acaattttga atcatatcct gtggccatac tccaacttgt
     6541 acttctccta tatgagcttt ttgtaagaaa tacatacata ttcttgactg accaattcct
     6601 ccaccaattg tatacggaag ttcgccattt aaaagcatct tatgatagtc aaactctttt
     6661 ctctcttcac atccagcaag tttcaattgt ctatcaagag attcttcatc aactctaatt
     6721 cccattgaag ataattctaa tgctgaatct aaaacaggat tgtaaaataa tatatcccca
     6781 ttcaattccc aatcatcata atcaggactt cttccatcat gtttttctcc tgatattaat
     6841 gtttttccta tttgagttat aaatactgct ttcttctctt ttacaatttt atcttctctt
     6901 tcctttggtg ataattctgg atacatatct tctaactctt gtgatgttat aaaagttatt
     6961 tcttctggta atatttcttt tataccttca tacatactac atacaaaaat ttcagtatct
     7021 ttaaatactc catacaatct ttttacaaca tcttttagag tcttttcatt tctactactt
     7081 ttatctataa ccaattccca atcccattgg tctacataaa gagaatgtat attgtctaat
     7141 tcttcatttt ttcttattgc attcatatct gtatataaac cactaccaac ttcaaaccca
     7201 tattttttaa gggctaatct tttccatttt gcaagtgatt gaacaatttc tgcattttta
     7261 ttcataaatg gtatatcaaa gcttacagcc ttttctccat ttaaattatc atttgcacct
     7321 gtttctggca atacaaataa cggagaagaa actcttataa gatttaattc attacttagt
     7381 ctcatttcaa aaaaatcttt taattttttt atagctattt gtgtatcaat tacgttcaaa
     7441 ctacttttat aatcctttgg tattattgtg ctcatatcat tcactcccta tatttatata
     7501 tttttccatt aaaaaaaccc ttcatcccta tataaaaggg acgaaaggtt gcttttccgc
     7561 ggtaccaccc tgattagttt gaaataaatt caaacccagc tcacaatgta cagatattac
     7621 tcgatacacc ccaatatgat aacggtttgg ttccgactaa gcctactctt ttaaaatttc
     7681 ggttagcaac tccaggatgt tcttcactat agacttcata ttggtctcac accatcacca
     7741 actcgcttta attacattct ataactactc ttcctttcaa agtctttaag agtttaattc
     7801 aataaaattt agctctctta tgttttctat ttattcataa tagtatgata agtgattagt
     7861 taaaatttgt caagtttatt atttttttat ttttaaacac tttataaatt tttacctata
     7921 ctttataaat tttcaaatat agtctttaat ctgtccatac attcttttaa taaagactgt
     7981 ggacaagcta catttattct ttcaaatcct attccttctt ttccaaatat ataaccttca
     8041 tcaaataata cattagcttt tttaaacatt aaatcttcaa gttctacttc atttaatcca
     8101 taagcagtaa aatctaacca tgctagatat gttccttctg gataaacaag ttttactttt
     8161 ggtaggtttt cttttaagta ttcatcaata aattgtatat tattatctaa gtaatcattt
     8221 aattcattaa gccagtcttc actttcatta tatgctgcta ttgtagctac aatagcaaag
     8281 ggatttggtg aagttgcaac tccaacagct gtaacctctt cttgatattt cttttttagt
     8341 tcatcattat tcactattat attagacaac tgcattcctg ccagattaaa tgttttactt
     8401 ggagctatac atgtaactat tttatccttg tactctgggc ataatgactg taaaggagta
     8461 tgtttaaatc cttttctaat taaatcagaa tgtatctcat cagctattat ccataggtca
     8521 tattttttac atatttctac aacctttttt aattcatctt ctctccaaac tcttccaaca
     8581 gggttgtgag gactacaaag tattagtact ttattgttgc tacacttagc tttttcttct
     8641 aaatcatcat aatccatcct ataagtacca ttctcatata tcagaggatt gtttacaacg
     8701 tccctattat tgctctttat cttagcttca aatgggtaat acactggttt ttggactatt
     8761 acaccttcat tagaattagt taatattctt actaaaatag caacagcagg tacaacacca
     8821 ggactaaaaa taatatcttt tctacttatt tcccagttaa atctcctttt ataccaatca
     8881 caaactgcat taaagtattt atcactatca tttgaactat atccaaatat tttatgttca
     8941 acttttttac gtaatgcttc tattattggt tctgcacatt caaaatccat atcagcaatc
     9001 caaaaaggca aaccatcttt ttgtgctttt gttgaacaat tagttctaaa atcccatttt
     9061 acacaatctg ttcctactct atctattact ctgtcaaaat tatacttact cattttttcc
     9121 ctcctaagaa taaaattttt ataattgtta agtctattac tagatgcact ttggtattat
     9181 tttacaattc ttaatattat aataatatca cttttttgtc caatagattt aaaatataat
     9241 tacttttcca tcatattttt tcaatattcc aaaatcagtt tcattatatc aaaataagat
     9301 tgctcatatt aattattttc taattaacat aagcaatctt tttctataac ttctataatt
     9361 ataattttaa atgtagaatt attagtttgg atgttatccc aatctctttt caaccaatct
     9421 acaccatata tctacaccat attctaaagc tttcgtatca aatgttacat ttggattatg
     9481 aaatcctggt gtagcatcag ctcctaatcc aatatatgta gttttacatt ttagtttttg
     9541 tgtataataa tggaagtctt ctccaccaga atttttaaat atacctatag atgcatcagc
     9601 tcccaataca tcttttatag tttcttcagc caagtttaca agttcttcat caaattctgc
     9661 tgctggtact ccatctttgg aaattagtac agcttctgca tttatagaac ttacaacagt
     9721 ttttacagca tgtattgctt tttcttttaa ttcttctatt atatcattac tttctgccct
     9781 tatatctaaa gccataaatg cttcatctgg tatattatta taagtttctc ccttaactga
     9841 tatatttgtt acttttatag aatttggtat agctggattt actcttattg catttattgc
     9901 attcactgct aaacagacag catctactgc atttactcct aaatgtggtt tagaaccatg
     9961 acaagtgata cctttaatcg taaattccat tatacagcta gcacaatggt acattgcagg
    10021 tatcgccttt cccaacctac aatcttgtcc aggtcttaaa tgcatcccta ccatttcttc
    10081 tacatcatcc aacaaaccag aatcaattac tcttaatgct ccatataatt tttcttcacc
    10141 tggttgaaaa agtattttta acttacctct ttttactaaa tttctctctt ttattaactt
    10201 tgcagctgtt aaaagcatag aagtatgacc atcatgacca caagcatgta tcattttctt
    10261 ttctccatct atgataaatt ccaatgcatc catatctgct cttatagcaa atactggtcc
    10321 ttctttttta gaatctatag ttactataat accagttcct gcaactcctt tattaatatt
    10381 ttcaaacccc attttttcaa gctcttttaa aacaaactct gatgtcttat attcttcaaa
    10441 tcctttttct ggtatagtgt gtagataatc atacacttta tgtacatact caatataatc
    10501 tttgttagtt cccataatct agctcctcct taaaatttaa actttccatt tatattaaat
    10561 ttttattata attacctata ctacgaaata attcattata agcattgcaa tacaaccatt
    10621 taaagttgct ataacaaaca tatgtggata atatttagtt tttaactcag ctgttcctaa
    10681 aagtctcccc ataaattgta tctgcgctcc tgaaagcaat aacattggca atagaattgc
    10741 aacatgtgct tgacttattt gacctgctgt aaataaacct gcgattactc cagatgctcc
    10801 tcccattgat ataaatattg tcattatagc tgttgctgca acacctggaa gtccaaagat
    10861 actcattaca ggtgtaaata ttgtccctat tacatccaaa agacctgtta agttgaacac
    10921 agaaattata gcaaaaccga agatgacatt tggagccatt gaatttgtac ttatttggaa
    10981 tccatttcta gctcctccaa taaacgcatc tatcacattt ccttttttaa cttttacttc
    11041 tttttttgct acctcactca tgttaagctt cccccttgtc aaatttattt aaatataatc
    11101 taagtaagtt cgccccaaca aacttcataa ttaatattaa agcaagtggt ataaatattg
    11161 gtacatctcc tataaatgga aatagtactg ctccagaagc aaagaaattc gtaattgcag
    11221 acgcacctga aaattgaaat gcacaaaata cagctttttg tttttcatct atatacccat
    11281 catctttaag ttctttagtc atagatgcag ctgcatctgt agactgtaaa ctagctatca
    11341 aagttagtcc tgcaaatcca ggtaacccta ataatggctt taatagtgga ctaagaagct
    11401 tttgagctgc cttcattcca tctaagtggt ctataactct aactactcca agtgcaaaca
    11461 taactgatgg tattaatgtc aatgcatata gccatccatc tcgagggcca gtacctccat
    11521 cacctctaaa gtttgcagct aatgtaccta caccatcatt aattgttccc aatgttccaa
    11581 aagaacctaa tacattgtta aaatccaata cttttaatgg tcctggtgca tctttaaata
    11641 ttcctgaaaa aaataatgct cccaatatta atgctacata cccataccat tttacctcaa
    11701 ctttttctgt tgttgattct aatttactca tataaattct ccctcctgtt taaatgaatt
    11761 ttattagtat acttgtttaa taagcaaaac ttatgccaat accttaatga taaagccttt
    11821 gtatttattt ttttattgta aatttattcc agtgattaaa tagcttataa ttacttactt
    11881 ttcaagttta aaaatataat atttttttat taattattta aaatatacat tacaatataa
    11941 aaagctattt tccttatctt gcaaaaataa tttcaaaata ggtttttata ggataaaatc
    12001 ctaaaaaaac ctattattta atcaaatata taaatttata ctactatttg ttgtataata
    12061 taacttgcct tatactttaa atactttaaa aataaatact attttgttag gagaaataaa
    12121 atggataaaa agaagttaat ccttataaca tatgataaat tgaatagtga ccactacaaa
    12181 gaagaattaa caaacttttt tggagacgaa ataatcatag aaacacaaaa tatacttgat
    12241 ggaataaaag aaaatttaga gggagatgta gtgctttctc tatcaccttt aactagtaat
    12301 tttttaataa aacattttaa ggaagatatt gaaattattc atggaacgaa agcactttca
    12361 aaacttggat atgaaaaaat gatgaaactg cctccaggta caaaatcgtt acttatgaca
    12421 accaataaaa cttcagcatt tgagatggct acttatctat ataaaattgg tataaatcat
    12481 atagattttg taccgacata tccagattgt gatgaaatat atgatttaga tacagctata
    12541 acaccaggtc agattagatt tataccaaaa tacataaaaa atattgttga tttaggttgg
    12601 agaaaaatct cattagatac atatatgtca ttattagtag tattaaaact aaaaaatgag
    12661 aaatttatag aaaaattata caaattgtcc aaggagacac taagtcatga tttcttaaat
    12721 acatcattag ataatatatc taaattaaaa actatacttt acatgaccat tgatgaaata
    12781 ggtgatggtc taatattttt caataccttt aataaggtta cttttgtaaa taagtcttta
    12841 cttaatatgt tggaactaga tgaaaaactt ataaaatcac cttccttaat ggaatatatg
    12901 ccaaagagtt tcttagataa aattacaaag aacttaaata tagataatat gattatatac
    12961 atagacgaaa tagataaaaa attcatactt tctaaaaaac cattttatct ttataaaaat
    13021 atagagggtt gcttaattac attaaaagat gtaaacaata tagaaatatt agaacaaaaa
    13081 ataagaagtg attctgtcaa aagaggttat gtagctaaat ataaatttaa taatattatt
    13141 ggaaatagtt ctattataaa agattgtatt aaaaaggcaa aaaaaatggc tcttacagat
    13201 aatcctatat taataacagg tgaaacagga acaggtaaag aagcctttac tcagtcaata
    13261 cacaatcatt caaatagaaa aaataaacct tttgtagcaa taaattgtgc ttcattacca
    13321 agtgaattac ttgaaagtga actatttgga tatgaagatg gttcttttac tggagcaaaa
    13381 aaaggtggaa aaaaaggatt attcgaatta gctcatactg gcactatatt tttagatgag
    13441 ataggagata tgccacatga tttacaggtt aaacttttaa gagtcttaca agaaaaagaa
    13501 attagaaaaa ttggtggtac aagtattatt ccaattgatg tgagaatatt agctgccaca
    13561 aataaagatt tagagaaatt aattatagaa aataaattta ggatggattt attttatagg
    13621 ataagtatgt tcactttaga tttacctcct cttagaaaga gattagaaga tatacctctt
    13681 cttttagaat catttttaaa agaacttccc tataaaaaca taaaactcga taagagtctt
    13741 ttagaagctt taaattcata cacttggatg gggaatatta gagaactgag aaattgtgtt
    13801 gaatatatgg cttacatggg ttccaattat cttacaataa atgatttacc tcaaaacata
    13861 tcttcaaaat taaataataa tcatatgtca agtaatatgt ccatattcaa tgatttaaac
    13921 caatatgaca aaaacatatg catttctatc ctaaaatcat tgcacatgaa accaatgggg
    13981 aggacaaagt taatgaaatt tatggaatat aatgtcacag aatacgaagt aagaaatatg
    14041 ctagaatatt tgacacgcaa tggatactta atttctagta agggaagaaa aggctcatca
    14101 ataactgaga aaggcataaa aataatagaa aataatataa tttaaaccta tagtaattca
    14161 tctttacttt atcaaaattt atatataatt ataatttttt atttttctat aaatttttta
    14221 taaaataata aaacttattt atattaaaaa cgaagctatc tcaacatatg gataagcttc
    14281 tttttaattg ttaaaaagat aaaaatatat ttgatttaac agaaaaagag tgcctcataa
    14341 actaaaaagt tcattttgag acacgtcctt aatattaata aattaattgg cttaaaatta
    14401 ctgattgata attataaagt acctctgtta aaggagtaaa ttctgaaccg attccaaatc
    14461 ttaattctgt aaacgcctct aaataatctg ggtcacatac atcttcttgt gttaaatctt
    14521 gtttcaccat ttcagtagca agataaattg ctactgatgg tagaaaagca tccattggca
    14581 taggatatcc accaagtcta tttgacatgc caaaagcctt agccttttca cttatcatgt
    14641 cgtttatttt atcataattt tgagcatctt tttcagatat ttttagaccc ataacactag
    14701 gatatgtttg tattggtgat ggattgcttt gctctgcaac tatatacttt aattctaaag
    14761 ccttagtcaa tataacttca tccatatact catttacacc aaatacattt atatcttttc
    14821 catatttttt aacttgtttt tcaatatctt catttaaaaa ctgcttaact ttgtttttgt
    14881 cttcttcagt atttatattt ggagtattaa cttgtacaaa tggcaatcct atatttttgc
    14941 aagtctcttt aatcatttct aatcttttgg ctatattaac atctttcaaa tcatctgtag
    15001 aggcataatg aataaaagct tttgctccca tttcttttga acgttcagct aatacttttc
    15061 ctctttcctc tgcactaacc cctagattca catcaacaaa ttggcttaat tggtttttat
    15121 catctcccat aggggcagaa atagttatta tttctggacg cttttccttt accttttgta
    15181 aagcaggtaa aagccctgct tggtcagttg aaactacaat agcctgaact tctttatcgt
    15241 cagccaactt tactatctta ttaatagcac tgtctatatt agaagtaaaa ttctctggca
    15301 atacaacatg ctttatagtt tgtttaactt tagtttgacc atctttgtct ttatcaattt
    15361 tattctcttc ctcatattct tttgccatct cttccaccat attgtattgt actttatttt
    15421 cagataatgg ttgagttacc actgcaactt taaaatcatc tataattttc tctttatctt
    15481 tcttgcctac aagtaaacta caacctgtaa gtccaataat taaacatatg gatagcataa
    15541 atgctgttat ttttctaagc attaattaaa acctcctaaa tttgtacatt cttatcacta
    15601 ttactaattt tatcatactt caaagaaaaa ttatattata aaaaattata tacatgctat
    15661 tataatccaa tctaaaaagt actatgggta gattaggttt agattaaacc tacccatagt
    15721 actaaaaatt aatatattat aaaattaaat taattacaaa ttattatgct acttgtttgt
    15781 tgctttttat aaagttacca actattccac ccaatatagc tggtattacc cagttaaagc
    15841 ccaaacctgc aagtggcaag ctttgtataa atggaacagc tcctgtcatt ccatttatag
    15901 ctgataataa actaatcact aatgtaacat atgctgcacc tttaaaagca ttatcattct
    15961 ttatatgttt accagccaac tccataacaa ctattgctaa taaaacagga tatatagttt
    16021 gtaggattgg aactgatatg gctattatag aatcaacacc taaatttgat ataagcatac
    16081 tagctacaca tatacctata accagttgtt tataactcgc cttacttatt ccttcaaaat
    16141 aagttgatat tgatgctgtt aaacctgtag aagtagtcag acaggcaagt gccacaatta
    16201 cacctaaaat tacctttcct acttgaccat ataacaatat actagttatt tcaactaata
    16261 aagatgcttg aggtatacta gtatcatata aagttgaaac tgttgcacct aaataagtaa
    16321 gaccaccata tactactgct agaaatacac aggctacaaa accagcttta atagtcattc
    16381 ctatttggtc tttatctgat gtatatcctt tagctattat agacatcaac attatagttg
    16441 ccatagatgc tgcacctaga gcgtccattg tttgataacc ttgtgatata ccttcaccaa
    16501 atagattttt atctatcata tgctcaggac tcataggtcc tgctggagaa attataccct
    16561 ttactattaa aacaaataat actaataata acgctggagt taaatactta cctattatgt
    16621 ctataacctt agaagaccta atagtaaatg ctaaagttag cccaaagaag attattgaaa
    16681 atacagcttt agctactgga ccatggtctc caaatatagg cataataccc atttcatatg
    16741 ttgttgcgca agttcttggt attacaagaa gtgctaaaca tgctagagca gctacactca
    16801 ttactcttgc taacccttta ccagctctcc taagtacatc atccatgctt cccttacatc
    16861 ttgctgttgc tataataaca agcattgcta taccagcatc tgcaacaaca aatccaataa
    16921 gaggaattaa ccagtctttg ccggattcca tccctaaaaa tggaggaaac ataagatttc
    16981 ctgcaccaaa gaacatagaa aataaagcaa aaccaactat aaaaatatct ctcatctgct
    17041 tactcacact aaattacccc ctttgaaaat attttgaata ttaagataat cttactttat
    17101 tttcagaaaa atgtcaatct ggtaaaaatt cacacattgt taacgttttc ccaatttatt
    17161 ttatgaatat ttttttcatt ttatgataat tcccatcaat ttcgttaaat caattgcttg
    17221 tgacacatca aaaattgctc ctctaatatc atctatacca atctcaattc cctcaatatt
    17281 attagtagta aaatctatgc cagaaagttt agttccatta aagtttgctt tttttaaatt
    17341 tgtatactca aatactagat tgtcaccttt tacttcttga aatacagaat tttcaaaatt
    17401 tgaatctaag atagaaacta tcttaaattt agagaatgaa aagttagaat acttacctaa
    17461 aacatccttg aacaaaacgt ttttaagtat acaatcatca aatctaactc ctatcaattt
    17521 actattttta aattcaactc tgtatatact tcctccatca aaaaatgtat ttgataaatt
    17581 gcagttttca aataccacat caagtaaatc aatctttttt aaattacaat tttcaaaaga
    17641 cacatttcta aatatacaag attcaaagct aactcttaca gaatctaaat cacttataac
    17701 ttcattttct ataattttat taaatatttt ttcatcatta aatatatctt caagtatatc
    17761 ttcaagattt tcaataattt ctaattctcc tgtaaattta ggtttttgca ctttaataat
    17821 tttagtcatt ttcccctcca ctataaatta tattattttt gatattattt tttttaatat
    17881 attaacttaa tatacatatt tgatatatta aaaagttttt acgaaatatc catgtattca
    17941 gtacttttat aattatatca caaactcatt tataataaac ttccattaat agtataaaac
    18001 aagttagaat aattgacatt atactcatac atattcaatt attggaaaaa gtaatttttt
    18061 tcaataaata accttaattt tttataaaat cgacaaaaaa acgaataatt ttattatatt
    18121 tattgtaaat aatacaatat tacgatataa tacaacagaa aaacaaatag aaaatgttca
    18181 tataatatta gtaatacggt ctaatagttt ctacaaactc accttaaatg ggttttctat
    18241 gaatagaatg tcaactctga atatatgttc tattctatct atatgaatat atattttttt
    18301 gtttatatct taaagttttt tggaggtaaa aatggtacca aacactaaca atgtacaacc
    18361 aggtggaaat aattcatcat taagcttttt agacaaaatg tttaaattta gcgaacgtgg
    18421 ctctagcctt aaaacagaag taataggtgg agttactaca ttcttaacaa tggcatacat
    18481 agttttcgta aatccagcaa tattatctga agctggaatg gataaagccg cccttataac
    18541 agttaccata ctagctacag ccattggtac acttatattt gcgattttag gaaatgcacc
    18601 atttgctcta gctcctggta tgggattaaa tgcatttttc acatactctt tggttatagg
    18661 tgcaggaatc ccatggcaaa caggtcttgg ggtagttttt ctatctggat gtgtcttctt
    18721 tacactcgca gtaacaggtc ttcgaaagac aattgcagac gcaattccta gagaattggc
    18781 agtagcatct tcagcaggta taggtctttt ccttgctttt gttggtttaa aaaatatggg
    18841 tgttataata gctaatccag atactatagt ttcacttgca gattttacac caacagtttt
    18901 aatagccctt tttgctctta tcttaatggc aatactagaa tataaaaaag ttaaaggtgg
    18961 aatattgata agtatagtag ttgcaacaat aatatctgtt atatttggat ttgtggagtt
    19021 gccaacagca ataatctcaa cacctcctag tatagcacca gttgctttta aattagatat
    19081 tgtaggtgca ttgaaattct cattaattgg acctatattc tctttcatgt ttatagacat
    19141 gtttgattca ttagcatttc ttattacttg ttgtaaacaa atgggtcttg aagatgagaa
    19201 tggtgaaata aaagatttag gaagaatgtt atatgcagat gtttcttcta ctttaatagg
    19261 ctcattctta ggaactagta ctgttacaac atttggagaa tctgcttctg gtattgcagc
    19321 aggtgcaaga acaggacttg cttcaatagt aacagcagca ttattcttag ctacacttat
    19381 atttacacca ttacttggta tagttcctac ttacgctgca gctcctgcac ttgtaatggt
    19441 tggtgtgttt atgtttgaaa gcataaaaca gattgatttt gctgatagca aaatagctgt
    19501 acctgctttt gttacagttt tattgatgcc tttaacttat agtataagta ttggtttatg
    19561 ctttggattt atttcatata taataatgca tgtagtagct aaagaaactg ataaaatcag
    19621 tattacactt tggattatag gtttacttgc agttgtaaac ttagttctaa gttaatattt
    19681 ttaaatagtt ctaggtcaat attttaaatt atatagaata aaatcttatt tattttaaat
    19741 ttataaatag caaaatcact ttatatgtaa attgattttg ctattaactt tgtatagaaa
    19801 atttattatc ataatatata gatataaaaa tatatagaaa aggagtggtg tttatgtcac
    19861 aattacttat aaaaaatgca tatctaatta ctatgaatgc agatagagag gtatatgaaa
    19921 atggaagcat acttattgaa aataatatta taaaagaggt tggtaagttt gatattaata
    19981 ttgtagataa agatgcagag atatatgatg ctaaaggaaa aatactaatg ccagggctag
    20041 tcaatactca tgttcactta tctcaacaat taggacgtgg tcttgcagat gatgttgtac
    20101 ttttaacttg gcttagagaa agaatttggc catacgaaag tagcttcaat tatgaagatt
    20161 ccttgatttc atctactgca tgttgtgttg aattgataaa atctggtgtc actacatttt
    20221 tagaatctgg tggtcaatat gtagatgcta tggttgaagc tgttgataaa actggactta
    20281 gagcctgttt agctaaatca gttatggata caggagatgg tcttcctgag gcatggcaaa
    20341 aaactactga tgaggaaatc aacactcaat tagacttatt ccataagttt aataatacat
    20401 ctaatgagag aattaaaata tggtttgcat taagaactat ttttaataca actgaagaat
    20461 tacttgttag aactaaagaa ctagctgata aatacaatac aggtatacat atgcatattg
    20521 ctgaaatagc tgatgaaatt tcatttgtaa agcaaaacaa tggagttgga acagttgaat
    20581 accttgataa actaggtgta ttaggaccaa atcttttagc tgctcataca gtttggctta
    20641 ctcataagga aattgattta tttagattac atgatgtaaa agtttctcat gacccaggtt
    20701 cagctatgaa agtggttcta gggtttgcat caattcctga aatgttagaa aaaggaatac
    20761 ctgtttcaat tggtactgat ggagctcctt caaataatag aatggacctt atgagggaca
    20821 tgtatcttac atcactaata cataaaggaa gaactttaaa cccaactgtc gtaccagctg
    20881 aagaagtctt agaaatggct actataaatg gagcaaaatg tgctttacta gaaaaagaaa
    20941 ttggtagtct tgaagtagga aaaaaagctg atatgattat tctaaatcca gatacaattc
    21001 attgtttacc aatgcataat ccaattggaa atatagttta ttctatgact agtgaaaatg
    21061 tagactctac tatttgtgat ggtaaatggt tgatgaagga aagaaaagta ttagttgtag
    21121 atgaatctga attattaaat aaagttaaaa aacaagcttc taaaataaaa gataaagcag
    21181 aagttaaaat tccatctaaa tttaaaatta taaaataaca taaatttcaa ttgttttgta
    21241 aatatgaata tataaatata tttatataca tcatatatac aaaacaattg tttttttatt
    21301 ttatattcat tagcattgct cataaaatac accataattc cagaaaaaat aaccaatcag
    21361 ttaggattac atcatctaag caatatataa acgtcttagt ttataagttt tattctactc
    21421 ttatctaaaa ccatctttaa agagttttca actgtttcaa ttacatcttt ataactcaag
    21481 tacacatttg cgtagataca atcttcatat aaattatatt cagttactct acaatacagc
    21541 agttttcctc catctaataa attgctcaaa atatagtttt cttttcttgg gacatagcct
    21601 atcacatatc cagtttcacc aactatttta atagcttttt catcatatag attatctttc
    21661 tctctagtta aatagtatat cttacttttc tccaattctt tttcaagata ttctctattt
    21721 ctaaaccttg aaccagcaac agctattgaa gtcaaataga tatccttacc atgttcatct
    21781 acttttattt tttcaatatt tacaaattct ttttttaaat tattactttc atataatact
    21841 cttcttaatt catccttcaa tataacattt ttttcttttt catacgcttc tttaacaatt
    21901 tttaattctt caagagatag tttctctctg tatcttttta agatatttat ggattctttt
    21961 cttacttcat ttatatttcc aaatattcct tttaaagata tattctttcc ttcaggatat
    22021 agtgatttac ttcctttaag cacaataaaa aataacatat catcataata ttcttgagtc
    22081 aatttatctt tttctatatc tttcattttt ccaattaaat catctatttt aaacgtctct
    22141 tcaaaaaaat ttaataattt taatctagaa cttgtcgttc cctttttaga tatatgccaa
    22201 tatacctcaa aatctctaat atctctattt agatatggca acaagtcatc aaaagataag
    22261 tttacatcat aaatttctga catttttata atatcttctg tttttccact agcatctctt
    22321 aatgcttcaa aatatatatt tttccatttt tcactgagca agataccatt tatttctttt
    22381 tcaaattcaa tcttattgca ctctatattt tcatctttta agacgtttat agctattagg
    22441 aaaattgaat ataaactttc aaagttagtc ccataattta ctacagtagg taaaaatcga
    22501 ttcactaatt ctaatttatt acctatatat ttaaattcta cacttagcaa gtaattgcat
    22561 ataagacgtg ctaaattatt tatcttttct ttatctaaat ctcgtttttc aagatattca
    22621 ttcaaatcta caattgaaat aatatagttc atcaataatc gttcgtactt tgtatcctta
    22681 taaccatctt caattagata ggaatttatt ttagaatcta aattttccat attaacaata
    22741 gcaaatactt ttattgaacc tgtagccttt ttagataaat taaataaata tgtattgtaa
    22801 aattcaagtt ttttaatagt attactcaga taaaatacgt attctccaga tttagaaaat
    22861 gtatctacaa cttctcttaa gttttctaca tttagatatt tctcacttaa aactaaccct
    22921 agttttacat actcaacttt tttactctta tatattaatt ctttagttaa tttatataac
    22981 catttctcat caataatcat ttcttttacc aaagtattta tctttttcat aaaaatttcg
    23041 aaataacaaa ttaaagactt agaattacta ccctccaata aataaatttc attagattca
    23101 cttttataca tatttaaaat ttcatctact agattatttg caagctctac ttgttctctt
    23161 aacggtatac tttcatatat acataggtaa ttcatttcaa atgattcttt gtatccatca
    23221 taaccattaa attcctgcaa tcgcttaaaa acattctcca ccaaattatc ctcctttaca
    23281 tgatgtaatt tatattgatt atactactta tttcatcaag tttacattta tacgcaaaaa
    23341 aatattacta gaaattgaaa ttctagtaat atccttatta ttaattatgt aaatacccta
    23401 tcttataact gtaaataata tccttattaa ttttaattac ttttaataaa aatctatcct
    23461 agatttagac ttctctatgc atatattata ctaatattag acatcataat catacacttt
    23521 ttctttttca tatctcatgt ttacaaacaa tgcaagtaaa attccaacaa atgttattgc
    23581 cacattaaac aatacaacat attttggtcc attaactccc cctgattttg taataatacc
    23641 tgctatattt aaaataacat agtttgctat actagatgca atcataacta tagatgttat
    23701 ttttccttta ttagttggaa acatttcatt tgcagttgac gttgttaact gaagaacacc
    23761 tcctgctgct gaataaccta ttacaaatcc ccctaacata caaatagttg gagtttgaac
    23821 aaaatataca actaatagca taagtaaagc tattatagga tatataacta atattcttat
    23881 tggtttaata tattttttaa taagtaaaga agttattaat actgcacata ttactcccat
    23941 tgagtaaaag gattgaattt ttgtagggtc agccatccca tacaatttac ccaattcctg
    24001 attacaattt aaccataata caaatgtaga tgtacatgta aatccaatac aaacaagagc
    24061 tatacttgtt ggtgtaaatt tcatcttctc tgatttaact gttttgcctt tgttatcaat
    24121 aacattatta gctggtggaa atggtaacac agctatcaaa aaggcatcta ctacaataag
    24181 aatcgctgtt actataaaaa taactttaaa tgatattgaa tttgctgcaa caaatccaat
    24241 tataaaagga agcaaaaatt gtgctaaagc tattgtaaat ttagtaaaca tatttgctat
    24301 tgttcctaaa ctagcaaata tctccataca agaaggagtt acacaggtat ctaagaatga
    24361 atttgctgcc cctccaatta ttgcaaatac atatgctata taaaaatttg gtgaaaatac
    24421 aattccaaca aagaaaattg catgtagaaa gtttcctata agaccactta cttttcttcc
    24481 atacttatct gaaataggtc ctgcaaatgg ataagatata agtctcccta atcctagtgc
    24541 agcaataaca gctaaaacaa tacttacatc caatgttcca tctgataatg ttttagcccc
    24601 ccacattgat gaaaattctg gtttgtattg tcctaaaatt gaaattccaa taccaagtaa
    24661 aaaatatgaa aaatacaacg ctatggcagt aggataatat tttttattgt tcataatttt
    24721 tctcccattc ttgttaattt attaacattt ttgtgtacaa taaactcatc cccataatgg
    24781 atttaaagta tagtcagtta aagcttcaaa tttaactgga ctatgaggca aaacccacta
    24841 tgacaatttg taatttatta ttaaaaatat tgaaatacgt atgttttttc attgtttaag
    24901 atattactct tatctattta aagcataatt cattaacttc ttctacaggc atttctaatc
    24961 cagtatatag attaaaagct tctgctcctt ggtataataa cattcctaat ccaccaatag
    25021 ccttacatcc attagcttca gcatcttcaa tcatttttgt tttcttagga ttataaacga
    25081 catctgttac tactaaatct tttcttaaaa cactagtatc ttttatattt gtttcattgt
    25141 cataaggctt cataccaata agagttgcat ttgtcaatat atcacttgat tgtatctcct
    25201 cataaagttt gtttgtatct tctaaatcat atagattaac aacacattct ggtacatctt
    25261 tttttatatt ctcaactgtt tgctctgctc ttttataaaa atcatcctta ggattaaaga
    25321 tagatatttc tcttgctcca tctaaagcac attgtacttg tatagcagtt gctgcaccac
    25381 cagcacccat aattgtaatc tttttacctt taacttctac cccattttct tttaaattac
    25441 gaacatagcc tactccatct gttatatgac caactaactt tccattgtca tttacaattg
    25501 tattacatgc tccaattata cgagctgctg gagaaagttc atccatatat tctgtaacag
    25561 cagatttaca tggcatagta acattggctc cttttatatt aaaagttttt attgctaaaa
    25621 gtgcctcttt taccttatct actgtgatat caaaggctaa atatctataa tctaaatcta
    25681 atttcttaaa actataatta tacatcactg gtgatttaga gtgaccaacc ggtgtaccta
    25741 ttaatgcaaa taatcctgtt ctacctgaaa tttccataac tataatctcc tctcaatatt
    25801 atcattttct ttgcaaataa cctattcagt tatttacaaa gaaaaattta tttattattt
    25861 atatatttaa tgctacttca aaagcttctt ttgttgcatc taatgcccta cgagctttta
    25921 ctgcatcacc aacaatataa gtttctttta caacttcttt tataacttca cttaatgggt
    25981 cgtaattacg agaacccata gcaagtacta ctgaatcaaa accttttaaa tcatgctctg
    26041 tgccatctgt aagtaaatag tcgacaccat catcataaaa tttagaaact tttgcaccag
    26101 taatggtatg tatattataa ttttcaaaat cattcataag gaatttacgg tgttctgaaa
    26161 ttacatctgc accaacttct tctctaagct caataacagt aacctcatgt tcttgttctc
    26221 caaggaaggc tgccacttca catccaacca ttccacctcc aacaacaagt acttttttcc
    26281 cacaagattt tttaccatca agaaggtcta cagcatgaat taatcctgaa tcattgattc
    26341 ctggtatagg aagtacaagt ggagttgctc cagtagctat aataactaca tcaggtgatt
    26401 tctcctttat aagttctact gtagcttctg tattagtaca tatttttaca ccatattgat
    26461 tacattttga aatataactt ctaactaagt tagtaatatc tcctttccct ggaggatatg
    26521 cagctaaacg catttgccca ccaagaactt cagatttttc aactaaagtt acattatgtc
    26581 ctcttttagc acatacccat gctgcaagca tacctccaac acctccacca acaacaagaa
    26641 cttctttgct aatttcagct ggcttaagct ctgcttcatg tcctaatata ggatttgcaa
    26701 gacaggctat aggtttgcct tggaacatat ttggtacaca tccttgtaag catgcaatac
    26761 atggtatcat ttcgtctagt tttccatttt tagctttatt aggcatttct gggtcagcca
    26821 agctttgtct accgaatgct actaaatcac atcttccttg tctgaccatt aactcagcaa
    26881 actgtggctc tgtataacgt ccaactgtta ttactggtat actaacagct ttttttattt
    26941 cagtaactaa ttctgcactg aatccagcat gtaaagtcgt aggagcccac atatattcat
    27001 catgtatatg tatactacgt gaaacatgaa gtccatctat tccacaatct tctagatatg
    27061 ctgctattac tgaactatcg tgtacatcaa ttcctcctgg aacatcatca gtgctgttta
    27121 tacgacataa tattgctaga gaatttccta cttttttacg tatattttca atgataagac
    27181 gaggtaatct cattctattt tcaaaacatc caccaaactc atctacacgc ttatttgttc
    27241 tttgtgatat aaaactacta accagatatc catgagcgca gtgaacttca acagcatctg
    27301 caccagcttt ttgtgcacgt aatgcagcat ctccataaga ttctataagt tcatatattt
    27361 cttctgttgt tattgctagt ggtgtattac gaccatttga tgcaggtatt gcagaagcag
    27421 cttttaatgg atgtcctgct actttttcat ttccttcaga accagcatgt tgtaattgta
    27481 tagatacctt cgaacctgca tcatgacaag catctattac atttttaaaa ctatctattg
    27541 tgctatcatc atataaacaa ggcttacgag gacctccttt tgcttttttg tctacaacac
    27601 tagattctat agttattaat ccaaaaccac ctaaagcacg ctcaagataa taagccttag
    27661 aagtttcact taaagtacca tctgtgtttg caaaattgtt tcccataggg ggaactacaa
    27721 aacgatttgg aactgtcatt gaaccaattc ttataggtgt aaacatagaa ctaatcttca
    27781 taaggttttc cttcctttca agttttatat tataatactc tgtatatttt tatattatat
    27841 aagtttattt tggagatact tcagaccatg ctttatccaa aactttttta gttgcattaa
    27901 atacagtttc agcagcagtg gataaccctt tagatattag agaatcagat attacttctg
    27961 cccctatgac tcttggtgat atattgtgtt cttttattat tttcacaaat tcctctgtgt
    28021 ttccatatcc ttcacctgga gccaaacggt catgtagtga ttcatcccta agtttttcat
    28081 aaggagtttc taaaacatca cacacttgaa ttgatactat cttatctgct ggaacaggtg
    28141 caaggtcctc tgctgtctgc tttgctctag cccagtgcca agtatctaat ataagcattg
    28201 cattttcacg attacatcct ttcacaacac gccatgcagt agccaaatct ggtacaccac
    28261 tatatggcat aaactcaaga cctataataa gttcacctgc tctgtcacat aattctccca
    28321 atgcagttat aatttgttct tctggtatca tctctaataa tccacaattt atatgtttaa
    28381 cattaaataa gtgagccata tgataaacat tttgctcttt ttctctttga gcttccgttc
    28441 tatcttcttc tcttccccat tgagttatat attctacttc tgtaactttc atatcatatt
    28501 cattaagaat tgcaatcatt tcttcatctg taacacctgc attttttgca tcaatataat
    28561 tttcagcacg caatccaata ccatcaaacc cagcttcttt tgcaatacga actctatcac
    28621 ggaatgaaac ttctgttccc aatgtataag aactaattgt aattggaata ttaagatttt
    28681 tttgattatt catagcttaa atttgccccc tttattttct ttataattaa tagtattttt
    28741 gattttatat ttttatttat cctactagta gtattatttt accttaaaaa taaaagtatt
    28801 ttttgatttc tatttatcta gattttataa atttattgta tcacgagact ttactaataa
    28861 caaattgata atttcctatt aattcataga ttttgtttat agattttatt tataagataa
    28921 ttcttgtatt tttctattag gtcgtataaa tatatttatc ttataattca gacaaaaaac
    28981 gataaatttt tttaaaagct tttattctta ctctataatc atatataata aaaatgtaaa
    29041 aatatcttaa aggttaactt ctacaaatat ataaataaaa atacctatga aaggatgaac
    29101 aaaaattgaa tttatatcac ttacgctatt ttgttacatt ggcacattta gaacactata
    29161 caaaagcggc agaaaattta tctataacac aaccaagctt aagtcatgct atttctttac
    29221 tagaaaatga acttggggtg gctctatttg aaaaagaagg tagaaatata gtccttacta
    29281 agtatggaaa aatattttta aaagatgtgg aaaaatcatt agaaatttta gattcaagtg
    29341 taaaatcatt aaaaataact ggtactggtg aaggacaaat agacttagct tttcttagaa
    29401 cacttggtac tgattttatc ccagacatag tgcacaaatt tttaaaatct aatcctgcta
    29461 aatcaataga ttttaaattt catactggtg taactactga cattattcaa ggtcttaaag
    29521 aaagaaaata tgatattgca ttttgttcga aacttgaaaa agaaaaggga attgaattta
    29581 tacctgttgc caaacaagat ttagtactaa ttgtaccata tagtcatcca ctagctgcaa
    29641 aggatactat agatttaaaa gaaaccatac cttatcctca aatagtattt aatcagagaa
    29701 gtggtcttag atatataatt gatgatatgt ttaaaaaaat aaatcaacag cctaatatag
    29761 tctatgaagt agaggaagac caagtaattg ctggattagt tgcaaaaaat ttcggaattg
    29821 ctgtagtccc aaatatgaat atgcttagct ttacaaaagt aaaagtaatt cagattattc
    29881 acccaagttg ggaaagaaac ttttacttag catttataaa agatagatat ttgcctcctg
    29941 caattaaaaa ctttaaaaac tttgtcatta aaaacgctca attataattt attaataaag
    30001 gggttatttt taaatatcct cttttattct ttttataaaa taaaaaaatc tatactttta
    30061 aaagtataga ttcctaactg acagtcggca tccgagtctc agtaattatc gttatacaac
    30121 agcaactgga ttcagttcct gaataactag gttacttaca ttatatcaga ttttttaaaa
    30181 tttgtcaatt gtatctaacg cattttttca agccatggtc caacaggctc tacaatagga
    30241 acttctttaa atccccaatt tcttatatca ttcacactcg ttggagtata tattttgttt
    30301 ctgacagatt tgaatatttt gtttttttct ccatattgca tacactttgg attagcttct
    30361 attaaccctc ttcttgctct gctaagagga ttacataaaa tatctgctat ttctaaacct
    30421 tctctatagt cagcatcttc ttttttggca attataaaac ccttaacctt atctcttaat
    30481 tcttcatcta tatctaagta tattgttcca ttattaaata catcaaaaaa cgccttttgt
    30541 acttttaagt ttgagacatc atcccttgac tctataacta ttcgtgcaga ttctacagtt
    30601 tcattattta caaatgaata aaaattctga agaacatgta taaaagccac aacatacggt
    30661 tcctttgaag gtgcataata tttctttaat ctagctttat ctactgttac tgatattata
    30721 gtacactcta atctagatag aaataaaggc aactcatcat agaataactt aatctgtgaa
    30781 tcatcaaact tctcaaatgc tcttgatttt cttgatatat ctgtcaaatg tattcctatg
    30841 ttactggttc caaaacaata ctctttaaat gcatccattt ctttcctcaa agaacataca
    30901 taatcgcctt gacttactgc atatttatat tcaaaaatta ctcctgtcaa tgtaaatggg
    30961 tcttcatgat ttatatcagc tcttccactt tcatcaataa acattatata ttttttcttt
    31021 ataatcttac tcaccctctt aacatatgat gatagcatct atattcattt tatatgagtt
    31081 ttaaaaatat tccaagattt ttatataact taaacttaat tttatactaa aaaattgaga
    31141 tgtaaaacaa tcaagctttt attgattgaa tttcacatct caattataat tatttttcaa
    31201 tatatatttt tactattttg caagaatatc cattaattta tccattacta tataagatac
    31261 tccgcctcct atttgataag gagtttcaac ttttttattt gcaaatactc tttgttgctc
    31321 tgatgataat cctgaattat ccattaaaac tattggtgaa tgtaatttta cagctaatgg
    31381 tccagctgat aaagcatcta ctaaagcatc tgatttacta actaatacag cctgtaaatc
    31441 agtatctcca tagaattttt ctatcacttt ggcattagta tctactctac tatctccata
    31501 tattctattt cctgaaatat cttcagtagt tatatcattc atttttgcta ttactgaatc
    31561 gttaattgca cttggtccac ctataaagta tgcattttct aattttcttt ctgttagcca
    31621 tttataagat tcttctgtaa ttccatcttt attgactaat attataggtt gtttttcttc
    31681 cccagcttta gaagctacag ataaagcatc tgcctcccca tttccagaaa ccatatatgc
    31741 agtttttatt tcttctcgat ttccaagttc ttcagctatt cttgtagcag tttgatatct
    31801 atcttgacct gctattctct ttgtttctgt gttgaaagta gtttttattt cttgctctaa
    31861 tttagaactt attgcatttt ctccacctat caagattact gttttaggtg ctaataactt
    31921 catttcttca acagtttcag ttggtaaatt agctgtatta gtaagtagta ttgttgagtc
    31981 atagctactt gctaaaggag ttgctgttat accatcagct attgcattac catttactaa
    32041 tattacagta tctgaactat cagcattcca gtttccaact gaacttatct ttttagctgt
    32101 atcaaatcta tttgcaccta taattgtatc ccatgtagga tttatagttc catcttcatt
    32161 aaaagtataa tctttcccat ctattttttg agaaccagtt agcatctttc catctttgtc
    32221 taaatagtaa gacttaccat ctatttgttg ccatcctgtc tgcattattc ctgttttgtc
    32281 aaagtaatac ttattaccat ctatttcttg ccatcctgtt actgctaaac catcagatcc
    32341 tagatagtat ttctttccat tcttttcttg ccatcctgtt tgcattactc catctgagtt
    32401 aaagtaataa gtttttccgt ctattacttt ccatccagat actcttgctc catcagttcc
    32461 taaataatat tttttgttat taattgtttt ccatcctgtt tgcattactc ctgaagcatc
    32521 aaagtaatac atttttccat ttacttttag ccaactattc ttttggtatg ttccatcaga
    32581 ttttacataa tacttcttac cattttctgt tttccatcct gtttgattta gatttatctt
    32641 atattttgcc gctatcttta catagctgtc gttactataa tctataatta catctgaacc
    32701 tacaggaact tggtcaaata accatctaac atctttattg tgcattctta tacatccacc
    32761 agaaatatgc tttcctatag aactctcatt attatttcca tgtattccat atgtatctcc
    32821 ataagttcct tttaatgcta atcccatcca tctatctcct aatggatttc ttggacttcc
    32881 tccaggtata ttacctttat aatatggtct attttttatc ttgttaacta cttttgtttt
    32941 ccctgttggt gtttctgacc cctttttacc tgttgcaact ctaaattctt ttacaagttt
    33001 attatttaca aaatatccta gagtattttt tcttgaattt actataatca catgtttact
    33061 ggctgcgctt gattctggta cgccacatgc aaatactaaa actaaagtca gtgctagtac
    33121 taaatacttt tttagacttt ttttcactat aacttccccc tcttttagaa acattactac
    33181 atgtgtctaa ttataacata aaattaccaa taccttatat ttacaaattt gttacattta
    33241 tacatataag tatgtattct actcttcata cccattagga ttcatacttt gccatctcca
    33301 tgaatcttca cacatctctt caagttcata cttagctttc catccaagct ctttttctgc
    33361 ctttgaagaa tctgcataac acatggctac atctccagcc cttcttccta caatcttata
    33421 tggtatttct tttccacttg ctttagaaaa tgcttttact aaatcaagaa cactataccc
    33481 ctttccagtt cctaaattat aaacaacaag tcctggattt tcttctaatt tttgtaatgc
    33541 ttttacatga ccagcagcta aatctaatac atgtatatag tctctaactc cagttccatc
    33601 atgtgttgga tagtcattac cataaacact caattccttc aatttaccta ctgctatttt
    33661 tgttatataa ggcattagat tgctcggaac tccatttggt tcttcaccta ttctaccact
    33721 tttatgagca ccgactggat taaagtatct taaaagagct atatccaatg acttatctgc
    33781 ttttgatata tctactaaca tctgctctat cataagttta gttcttccat atggattggt
    33841 tacagaaagt ggaaaatcct ctaatatagg acaagtatgt gggtctccat atacagtagc
    33901 tgaagaacta aatacaaact ttttaacacc atattctctc attaattcaa ataaatttaa
    33961 ggtacttatt agattattgc tgtaatattc aattggtttt tctactgatt caccaactgc
    34021 ttttaaagct gcaaagtgta taattgattc aatattattt tctttaaata caacatgcat
    34081 ttcatcttta tttctgatat caatattata aaattttact ggttttttag ataattcctt
    34141 gattctatca accactattg agttgctatt acttaagtta tcaactataa caacttcata
    34201 acctgattct aaaagttcta ttgctgtatg gcttcctata tatcctgctc ctccagctac
    34261 taaaactgcc attgtaatta cctcttttct aatatttatt tttcacttac tttttttctc
    34321 atatagtcca ttaaatcatc ttttaaattt tctctcttta gagcaaagtc tattgttgcc
    34381 tctaaaaaac caattttatc tcccacatca tatcttctac cttcaaagtt ataagcataa
    34441 atagcctctt gttttccaag agtttgaagt gcatctgtca gttgtatctc tccaccttta
    34501 ccaggtgtct gtttctctaa tatgctaaat attgcaggag ttattatata tcttccaagt
    34561 atagctatat cagatggagc ttcttcaatt gcaggttttt caaccatatc tttaacttta
    34621 tatactctat cttcgatatg ttttacatcc aatataccat atttattagt atcttcttta
    34681 gctatctttt gaacacctaa tacagttgtt ttatactcat tataagtatc tattaattgt
    34741 tttaagcacg gaacatctga atctactata tcatcaccta ataatacggc aaaaggttca
    34801 tcacctataa aactttttgc acagtaaatt gcatgtccaa gaccttttgg ctctttttgt
    34861 cttatataat gaatatttac catatttgat atgtctctta ccatttctaa catctctgtc
    34921 ttaccttttt gctctagttc taactctaat tcaactgatt tatcaaaatg atcttctata
    34981 gattttttat ttctacctgt tattataaga atttcttcaa ttcccgattc tatagcttct
    35041 tctattatat actgtaaagt tggtttatct actataggaa gcatttcttt gggctgtgat
    35101 tttgttgctg gtaaaaatct agttcctagc cctgctgctg gtattattgc ttttttaact
    35161 gtcatctgca ttttttcacc cttcttcgta aattttctat tgtaccaaat taatatacca
    35221 tatttatact ttttcttcaa actctatatt atcccaatat ttaatgctat aagataatat
    35281 aaggtagatg aaatttcatc tacctttagt tttataagtt aatattgtat tgtttatatt
    35341 ttatccaaaa cttatacagg tactaaattt catctaagat gccttttata tcactatcta
    35401 tatcttcatc tagataagaa tctaaatctt ctaagtcaac ttctagttct tgatatgtca
    35461 tatcaacact gacatcctca tctatatcca tttctgatat taattctcct aaaatactat
    35521 ttccatcaga atctaaacca ctatctatat cttcatctat atagttttca tttaaaattt
    35581 catctaaatc aatctcgtaa taaacttcat tatttttttc atcccctgag tccaatccta
    35641 agaactcata gttaaaagtc caaacattgt atttacttga catactttat tcctcctgtg
    35701 aaaaacatat tttcatctag tttattggtt ccataatcaa cagagttata tattttttca
    35761 attatgtctt ttcttctttc ttttgtacta ttatttaatg tcaattgttt aaatacatat
    35821 gtatttgtta ttctctcgaa taatatttct tttatactat catcatcaat atttctatag
    35881 tttttccaca ataactcaaa taacttaaac atttccctac tagtttcatc tacataataa
    35941 ggatacatat tttctactaa atggtttaat cttgatatgg cttgttttaa aaatacatca
    36001 agacttgcac tttcactttc ttttaacatt ctataattgt atttcttctt atcttctcct
    36061 gaaattacaa agatgatatt gtctaaactt ttttcaactt catttaaata actgcttata
    36121 ttatatgata aatcagacaa tagattttta atctgcttaa acacttgtac atcaagttta
    36181 gtattatact tttcaaacaa atctatttct tgattttcta atattcttgg catatatgct
    36241 gacaataaat ctaaattgtt tctttgttct tgtatttgct tataaagtga atttataact
    36301 tctattctta tttcatcatt taactctttt tctaaaatct tatcaaattc agatttcata
    36361 tagtaaatat ttccaaagaa attaaatatt atcatatcat tcatagcttt agaccaattc
    36421 ttatatatca cttcaataga attttcttta agtagaggtt gctttgcata tataaataca
    36481 tcttgcataa gtttttgaac catactttta cttaagttta aaagtacttt ttgattatta
    36541 aattttattt ccgtttctac aaaattagaa ttttcgtttt caagttcttt attaattaca
    36601 atgtctttta tatcactatt ttttatagat atgatttctt tttgaaaaga ctttagaata
    36661 gtcaagctat cgaaatcttc ttgaataaaa aatggagcat gttttaatgt atatcccact
    36721 atttcatttt ctatatcttt taggtcatct atttgcataa tttctctatt tttatcattt
    36781 atatgtaggt taaatacatt tttattatca tataatatta tatcttctcc taaatttaac
    36841 ttagaacaat tatctttcgc tgactttaca atagtcttct tagatttctt atttataaaa
    36901 attaattggt cttgatattt tactaaaagc atgtttgtgt ttttataatc aaatccatct
    36961 atatttcctg atatcttgca gaatactatt tcacaatctt taggaagatt tcctattttt
    37021 tggttatctt taactctctc atttaaatca gatgggacta cactatctat attgttatta
    37081 aatatagata cagaccttac tataatcccc tctctttcca tatgaaagta tcccttcaat
    37141 gacactcttt tatcttctaa aatgtatctt tctatatcat caaaatttat ttcaaatcta
    37201 tttttcccac tgataaatat aaacttctca ttatctttat taattccacc catataaaac
    37261 ttattgtcat ctaaacttaa tatagtcata ttatccttta gatagaatat aaattctctt
    37321 tgacttttgc agattaaatc atattcatcc aaagttattt tcatagtcaa atttgctgta
    37381 acatttttac ttgaattttc actttttatg taaaaaggca agttatttcc atagacaatt
    37441 atttgagaat cttctaaaac tgcctctaat ttttctttat tgtctgaaaa actatatata
    37501 tattttacac ctgtatcctc attaacttca tctgatttgc tgattattat attccctaga
    37561 tttgttatat gaagcttttt atattctttt gagtatctac ttatgccact aagctcaaac
    37621 ccatctgaaa gctcatatat gattttttct cctaatttca acataccttc accacctaaa
    37681 cagaattata agtattttat aaaggtgaat aaaatgtctc aaaacaaata atattttttt
    37741 taatactttt tattctgaga catagtttct ccttttttat aatgctctaa gcaccttcaa
    37801 ctatttcttc ataaagttta tctaaatgtt taacatattc acgatatcca gatttgttct
    37861 ttaactcact tcttaaatct tcatattttt gtttaagttc ttcagcactc aaagaaggag
    37921 ctgattcttt taattcttct aacttataat catagtactt atcatacata gctatatatg
    37981 tatcaataga cttcttagct agttcattac atttttcttg tgcttctcca tattgagaat
    38041 aatcacctat ttttatttta tagtatccat tttcttgaat tattgttgaa gaaatacctt
    38101 tagcttcata ttcttttgct gtttcttttg ctgttttttc aactttaagt gaacctgcat
    38161 aaacagtgta atatacacct ttaccatctt tatatgcttc catttctttt gctttctttt
    38221 cagcttgtgc tttttcttga gcctcttttt cagcttttgc cttttcttct gcttcttttt
    38281 tctctttttc agcttgtgct tttttctctt cttcagttaa tttattgtta gcttgttgtt
    38341 gagttgatga ctttggaata ttctctttat catttttctt attgttgcct actgttataa
    38401 aatatcctac accaacaata accactataa ctactgctat aatagtttct tttaatctac
    38461 tcttcttaga aacaacctct tcatcatcta aagaatcttc tttatctgat acataatttt
    38521 tcatttcttt cttgttttct ctatatttgt ccttagaaga atttttagat tttttattac
    38581 tattttcttt ctttttaata ggtattacta ctgatttttc attttctaat ttttcttctt
    38641 ttatttcttt tgctttttta tcttctaagt ttttactttc tttttcaagc ttcgattcat
    38701 tatttgaaga tactttttca tctaagttat tatttattgg tttttctatt tttacactac
    38761 gttcttcttc tatatgttga cttggttctt cttgttttat ttctttcaat tcattttgct
    38821 ctatttcttc tgatttcact tgatgatttt caactggttt atctaaaatt atatctggag
    38881 aaatatcatt taaattctct tcttcttgtt cagcttcttc attgtctttt ttaataaatc
    38941 caaataattt agccttcttt ttactctttt tattttctgg ttcaattata tcttctttat
    39001 tgctgtcgta ctctttatct gtagtatctt tatcttctac aacgtcacct atatcttgat
    39061 tattactatc tacttcttct ttagtccctt cttcagcatc atcttctatt gaaatactac
    39121 ttctaaagca aatatcttta tctgcatttt ttatcatgta aatgtattct tcaatatcga
    39181 tatctttatt tcttctagtt ctttttgatg caatttcatg attttctaat tcactatagt
    39241 tatcttttat tagattctgt gctttattcc atgtatcttc actaatttct gttttatttt
    39301 taattacata gtccacaaat tccttaaaat tttcaggacc aaattctcca attatagtca
    39361 gtctcattgc accttctata aggtctctat ttcttctctt taaaaattct aatacaactg
    39421 ttgcacaagc aaaatcttta ataatttctt tgccaaaacc atatgcttca acagaatgaa
    39481 gttcttctaa tccacataac ttcttatatt cattgtgtat tgtttgagat atactttcat
    39541 tatttaagct atttaactta tctataaact tcgattctat atcatttgat tttgattcat
    39601 gttttacttt tgaattttta atttcattta gtagcatgtc attttctgaa ttatctttac
    39661 ctaattgcaa atcctcaatt tcagttgctt ttatttctac tttgttttgt tcgttattct
    39721 cttccacatt attaggtacc acatctttat tactagctaa tattttgttt acacagaata
    39781 taatttctct ttttaattct cttttcgcat ctgcttgatt tttaagaaat aaatctccac
    39841 attctttctt ttctaaactt atttttctaa gagatatatt aagaaggtct ttatataggt
    39901 ttaaaacctt ataatccttt tctaaacgtc ttcttctctc tgatagttcc attttatcat
    39961 aagctaaaaa actatttaaa tcattggctt ttagagagtc taatccctct ttcatagcct
    40021 tatttacata ctttctacat tttgctgtag ttaactctaa atctttttct aattcatcta
    40081 tttcaggtat gtacttatct actatccatt tgccaataag tggttctgta tttcttgcta
    40141 atgtcttata aaacaatata ttaaatttta aaagctctgc atattttgct tcattaaatt
    40201 ctactttttc aaatttcttt tccaaaacac catctagctt ttcatttatt ttagactctt
    40261 gttttaaaaa acttgctgtg acatttaaaa cagtgtttct tattatatta ttttcactca
    40321 aaaccttcac tccttaaatc aattatttta ttgtaatatt tacattgtgc aaagttgcta
    40381 atttgattat gatatctaaa taattattta ctaaatttgc tacatcttat atcatatcat
    40441 attatttaca tcattttact ttttcttttt atatctatct tatattatac catttttaat
    40501 atctgtacta acttatatta caatattcct caaaatttta aaagtagatt agacaaattt
    40561 acaaataaaa aaacatcagt ttaaactgat gtttttgact atcgaaataa ttaagcaagt
    40621 ctataagcgg agttctgtat tagataatca tctttctagg cctattgtca ccaataggct
    40681 caagcgacct acctaccgga gagtacgagc agcactcttt attcctatct tggtcttgct
    40741 ccaggtgggg tttacatagc caactagtcg cctagctgct ggtgagctct tacctcgcct
    40801 ttccatcctt accacaatag tggcggttta tttctgttgc actatcctta ggatcgctcc
    40861 cactaggcgt tacctagcac cctgctctat ggagctccga ctttcctcat gtaagattac
    40921 atgcgattat ctggcttact taatatttat tttacttagt tatgttaaca caaattcttt
    40981 tacatgtcaa agtaatatta tactaaaaat ttaaatttat tcaaaaatag tgtaatatag
    41041 atatataaac tacatttaac tcgaggtgat ctattgaaaa aattatatag aatagtaatt
    41101 aacattatac tagtattagt aatactttac agtggattta atatatactc aaaacttacc
    41161 aaatacaatc atgatactaa aatatcctca gaacttcaaa aaaaagaata taaaaaagag
    41221 gatttatcaa agattaacag tgattttaaa ttttggcttt ctgttgagaa tacaaatata
    41281 aattatccag ttgtacaatc taaagataat tcttactatc ttgacaaaga tttttataaa
    41341 aaagattcta tttctggtac actgtttatg gattatagaa ataaatctat tgatgacaaa
    41401 aatatcataa tatatggaca taatatgaaa aataaaacta tgttcaacaa cctaaataag
    41461 tttaaagatg ctgacttctt taaaaaaaat aataaaataa aaataacctt gaatggaaaa
    41521 gaatttttat atgatgtttt ttctgcttat attgtagaat ctgattatga ttatctaaaa
    41581 acaaatttta acaatgaatc tgattatcaa aattacataa atgatattac ttctaaatca
    41641 ttgtataaat ctcctattaa ggtaaatagc aatgataaaa tagttacact ttctacatgt
    41701 acctatgaat ttgatgatgc cagaatggtg attcatggta gattgattta aaactcacag
    41761 acacagttta aaacaataat tttaaactgt gtcatctttt atacctataa tttgtaaatt
    41821 agaccactaa aagtatttaa tacaaaataa caaatacatc aaacaataaa aaacaataaa
    41881 aaaagactat gctaaaaaca tagtctttac tggagctggt gataggaatc gaacctacaa
    41941 cctgctgatt acaagtcagc tgctctaccg ttgagccaca ccagcacacc tattttattg
    42001 taaattagca aaactaattt ttaaaagtta
//
LOCUS       DAYAOX010000009        41137 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.009, whole genome shotgun sequence.
ACCESSION   DAYAOX010000009 DAYAOX010000000
VERSION     DAYAOX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41137
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.009"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..1356)
                     /locus_tag="ACGI57_000383"
     CDS             complement(<1..1356)
                     /locus_tag="ACGI57_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFG9715471.1"
                     /translation="MVNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREK
                     IYNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDK
                     AAEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMD
                     AVKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGV
                     SGPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSL
                     APTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIP
                     VSEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGM
                     INNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSF
                     KN"
     gene            complement(1379..1648)
                     /locus_tag="ACGI57_000384"
     CDS             complement(1379..1648)
                     /locus_tag="ACGI57_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFG9715472.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(1938..3893)
                     /locus_tag="ACGI57_000385"
     CDS             complement(1938..3893)
                     /locus_tag="ACGI57_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715473.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKLVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(4076..5551)
                     /gene="spoIVA"
                     /locus_tag="ACGI57_000386"
     CDS             complement(4076..5551)
                     /gene="spoIVA"
                     /locus_tag="ACGI57_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HFG9715474.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(5723..7018)
                     /locus_tag="ACGI57_000387"
     CDS             complement(5723..7018)
                     /locus_tag="ACGI57_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFG9715475.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDESGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(7202..8647)
                     /locus_tag="ACGI57_000388"
     CDS             complement(7202..8647)
                     /locus_tag="ACGI57_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9715476.1"
                     /translation="MSLNKFEIGGITVKKKIGFWDLVFMNISALFGIRWIAKSTASSF
                     GLGLGSIPVWLLFAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLN
                     WTSKIFWYSSFLTFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGK
                     FFTNTGALGSTIPAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAII
                     FALSGAETTANFITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAAST
                     GILDALAKVAQDLGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQ
                     LTVTNKYNIPSNAVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFT
                     SYIRLRKTRPNEERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYE
                     IEMIGGGLFVIITGLMLWKNYEKKISNNSGC"
     gene            complement(8663..9802)
                     /locus_tag="ACGI57_000389"
     CDS             complement(8663..9802)
                     /locus_tag="ACGI57_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFG9715477.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            10121..10771
                     /locus_tag="ACGI57_000390"
     CDS             10121..10771
                     /locus_tag="ACGI57_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9715478.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(10928..11947)
                     /locus_tag="ACGI57_000391"
     CDS             complement(10928..11947)
                     /locus_tag="ACGI57_000391"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFG9715479.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(11978..12592)
                     /gene="plsY"
                     /locus_tag="ACGI57_000392"
     CDS             complement(11978..12592)
                     /gene="plsY"
                     /locus_tag="ACGI57_000392"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFG9715480.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(12627..13952)
                     /gene="der"
                     /locus_tag="ACGI57_000393"
     CDS             complement(12627..13952)
                     /gene="der"
                     /locus_tag="ACGI57_000393"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFG9715481.1"
                     /translation="MSISRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRI
                     FAEVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAA
                     DKEIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLD
                     EIVENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAID
                     TYFEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTE
                     QDTKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAK
                     TNQRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTD
                     IRPPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(13980..15308)
                     /locus_tag="ACGI57_000394"
     CDS             complement(13980..15308)
                     /locus_tag="ACGI57_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HFG9715482.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(15546..15746)
                     /locus_tag="ACGI57_000395"
     CDS             complement(15546..15746)
                     /locus_tag="ACGI57_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715483.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(15748..16680)
                     /locus_tag="ACGI57_000396"
     CDS             complement(15748..16680)
                     /locus_tag="ACGI57_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HFG9715484.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(16725..17657)
                     /locus_tag="ACGI57_000397"
     CDS             complement(16725..17657)
                     /locus_tag="ACGI57_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HFG9715485.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(17712..19001)
                     /locus_tag="ACGI57_000398"
     CDS             complement(17712..19001)
                     /locus_tag="ACGI57_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9715486.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(19030..19722)
                     /locus_tag="ACGI57_000399"
     CDS             complement(19030..19722)
                     /locus_tag="ACGI57_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9715487.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(19737..20498)
                     /gene="pgeF"
                     /locus_tag="ACGI57_000400"
     CDS             complement(19737..20498)
                     /gene="pgeF"
                     /locus_tag="ACGI57_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFG9715488.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(20495..20956)
                     /gene="nrdR"
                     /locus_tag="ACGI57_000401"
     CDS             complement(20495..20956)
                     /gene="nrdR"
                     /locus_tag="ACGI57_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFG9715489.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(20978..21241)
                     /locus_tag="ACGI57_000402"
     CDS             complement(20978..21241)
                     /locus_tag="ACGI57_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HFG9715490.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(21345..22118)
                     /gene="sigG"
                     /locus_tag="ACGI57_000403"
     CDS             complement(21345..22118)
                     /gene="sigG"
                     /locus_tag="ACGI57_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HFG9715491.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(22210..22932)
                     /gene="sigE"
                     /locus_tag="ACGI57_000404"
     CDS             complement(22210..22932)
                     /gene="sigE"
                     /locus_tag="ACGI57_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HFG9715492.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(22957..23802)
                     /locus_tag="ACGI57_000405"
     CDS             complement(22957..23802)
                     /locus_tag="ACGI57_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HFG9715493.1"
                     /translation="MVYIEYYVIENLVINYIIISCTSILTKRYNPKKNKLLGASLGGA
                     YSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLVNIFISGSTY
                     FIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTININLLEKSFCC
                     KALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQEIIGSLDDK
                     TSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDREYGAIMNPN
                     ILPGF"
     gene            complement(24119..25393)
                     /locus_tag="ACGI57_000406"
     CDS             complement(24119..25393)
                     /locus_tag="ACGI57_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFG9715494.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(25677..26834)
                     /gene="ftsZ"
                     /locus_tag="ACGI57_000407"
     CDS             complement(25677..26834)
                     /gene="ftsZ"
                     /locus_tag="ACGI57_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFG9715495.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(27024..27377)
                     /locus_tag="ACGI57_000408"
     CDS             complement(27024..27377)
                     /locus_tag="ACGI57_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HFG9715496.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(27476..28150)
                     /locus_tag="ACGI57_000409"
     CDS             complement(27476..28150)
                     /locus_tag="ACGI57_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFG9715497.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VDLFAK"
     gene            complement(28155..28853)
                     /locus_tag="ACGI57_000410"
     CDS             complement(28155..28853)
                     /locus_tag="ACGI57_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFG9715498.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(28876..29616)
                     /locus_tag="ACGI57_000411"
     CDS             complement(28876..29616)
                     /locus_tag="ACGI57_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HFG9715499.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(29886..31112)
                     /gene="murG"
                     /locus_tag="ACGI57_000412"
     CDS             complement(29886..31112)
                     /gene="murG"
                     /locus_tag="ACGI57_000412"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFG9715500.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(31130..32260)
                     /gene="spoVE"
                     /locus_tag="ACGI57_000413"
     CDS             complement(31130..32260)
                     /gene="spoVE"
                     /locus_tag="ACGI57_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HFG9715501.1"
                     /translation="MPKENLKKQIDIRVKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(32312..33667)
                     /gene="murD"
                     /locus_tag="ACGI57_000414"
     CDS             complement(32312..33667)
                     /gene="murD"
                     /locus_tag="ACGI57_000414"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFG9715502.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSDNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(33686..34654)
                     /gene="mraY"
                     /locus_tag="ACGI57_000415"
     CDS             complement(33686..34654)
                     /gene="mraY"
                     /locus_tag="ACGI57_000415"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFG9715503.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(34695..36068)
                     /gene="murF"
                     /locus_tag="ACGI57_000416"
     CDS             complement(34695..36068)
                     /gene="murF"
                     /locus_tag="ACGI57_000416"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFG9715504.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVIDGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(36235..38214)
                     /locus_tag="ACGI57_000417"
     CDS             complement(36235..38214)
                     /locus_tag="ACGI57_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HFG9715505.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(38226..38573)
                     /locus_tag="ACGI57_000418"
     CDS             complement(38226..38573)
                     /locus_tag="ACGI57_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715506.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(39054..39989)
                     /gene="rsmH"
                     /locus_tag="ACGI57_000419"
     CDS             complement(39054..39989)
                     /gene="rsmH"
                     /locus_tag="ACGI57_000419"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFG9715507.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(40019..40765)
                     /gene="lgt"
                     /locus_tag="ACGI57_000420"
     CDS             complement(40019..40765)
                     /gene="lgt"
                     /locus_tag="ACGI57_000420"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFG9715508.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
BASE COUNT        12680 a         7732 c         4281 g        16444 t
ORIGIN      
        1 atttttaaat gaatgtatag gagctggaat ccttcctcct ctttgagtaa ataattcaga
       61 tgaatttgta tttataggca ttactggtgc tcgtcctaaa agtcctccaa attctaccat
      121 atcacctaca tcacatcctg gggctggtat aattctaacg gctgtagtct tattattaat
      181 catacctatt gctgcctcat cagctatcat ggctccaagc gtacctgctg tagtatttcc
      241 tggcacagct atcatatcaa gacctacaga acaaatagca gtcattgctt caagcttatc
      301 tatacttaaa gcacctttaa taacagcatc aatcatacct gcatcttcag atactggtat
      361 aaacgctcca ctaagtcctc caacatgtga acatgccatt actccacctt ttttaacagc
      421 atcatttaaa agtgctaatg ctgctgtagt cccatgagta ccaacaactt caagacccat
      481 ttcctctaat atatttgcta ctgaatcacc aatagcaggt gtaggagcta gtgataagtc
      541 tattatccca aatggaacat ccaatctact agaagcttcc tttgccacaa gttgtccaac
      601 tcttgtaatt ttaaatgcag tctttttaac tgtttctgaa acaacatcaa aaggctctcc
      661 ctttactttc tctaaagctc tcttaacaac accagggcca cttacaccaa catttataat
      721 tctatcagcc tcaccaacac catggaaagc tcctgccata aatggattat cttctactgc
      781 attacaaaat actactaatt ttgcacaacc aaaacctttt gcatcttttg ttaattctgc
      841 agtttctttt atgatttctc ccatttcttt cactgcatcc atatttatac cacatcttgt
      901 agaacctaca tttactgaag cacatacttt attagtactt gctagagctt ttggaattga
      961 cttaattagt atcttatcac cttttgtata tcccttttga actaatgctg agaatccacc
     1021 aataaaatct atacctaaat cctctgcagc cttatccaat gtctgtgcaa actttacata
     1081 gtcattttca tctgttgcac ctgcaataat ggaaattgga gttactgata cccttttatt
     1141 tacaattggg ataccaaatt cacgctctat atcttttcca accttcacca aatctttagc
     1201 agaactcata atcttattgt atattttttc tctagcttta tctccatcag ggtctataca
     1261 atctaataaa gaaatcccca tagttatagt tcttatatcc aactgttctt catctatcat
     1321 ctttattgtt tcaagtatat ttcctacatt cactattatc acctcccctt actgtctact
     1381 acagattatg cattgaatta aatatttctt ctcgctgaat ttttacatcc actgataatt
     1441 tcttacctct ttcaaccaag tcctcaaccg ttttctcaaa agaatttaat gatttctcta
     1501 aatcaactat cataatcata gtaaaataat cctgtaatat tttttgtgat atatctacta
     1561 tgttgatatt ttgcttgtat aattcatcac taattcctgc cactatacct ggtttatcct
     1621 ttccaattac tgttaaaatt gctttcataa taatatcctc ccttattttt ttataaatct
     1681 tatattttta caaattgaac tatgttttaa catatatttc atttatttgt tagtaaacca
     1741 ctttttatgt atatatttaa tatattttaa tacctataca ttcatttgtc aatttttttt
     1801 caatattcta ttatttttat actatttaga aaattcatat attatatatg ttttctcaac
     1861 aataaataca gttttcataa taatgaaagg tattacttag caatcgcatg attttctaaa
     1921 taataccttt ctaaaaatta tttttttaat tttatgaatt taattatact ttttctagta
     1981 tcaggattta caattaatat agaaccaatc ataattggtg ttagtataca aaataaaaaa
     2041 ataatatctt tttttagatt atattcttta gattctaatt tataattatt atttattatt
     2101 ccatcataat cacttatatt tgacttattt ttatttgtat cttgattttt gttagaaata
     2161 ctttccctat aaggaaaaga atgttcattg gtaccaaata ctaattttga gcttaaatca
     2221 taattatcat cattttctgt aattgaattt ttattgtaaa atcctttttc tttatcagaa
     2281 ttattttctt tattaccgtc tttaggtata tcatgaggat ttttattttc attgctatca
     2341 gtacttgatt ctacatcatc tttaggagta tttggttgtt tctcatcaga attatcattt
     2401 tcattattat tattttcatt attattgttt tgattgttat tactatcatt attttcattt
     2461 ccattattcc catcttgatt gttatcatcc tcatctctat ccgggtcgtc tctacctcct
     2521 cctccacggt caccatcaat atcatcattt tcccgttcat catctggtgt ctcttggttg
     2581 tctccgcctc ccattataaa tcctggttta aaaactatat ttgaataccc ttcttttaat
     2641 cctcctacca cttttactct aaaacaacgc aacttaaaat cttcaaaaaa aagtatagct
     2701 tgatctctaa tatcctcctg ttcttcagat atccaattga ttccatcgct tgaatattca
     2761 acaaaaacct tacttgcact atacggcata tcataaaata aaacagctac atatccattt
     2821 tttgtatttt gaaattccag ttccaaattg tctattggtt ttttctctat aacagataca
     2881 ttcaaaatag gaactacatc atcatttatt ataatttttt ctttatttaa catttctgtt
     2941 ttaaactttc ctgttatatt aaaattttct cccttttcca acttttcata ataatcagta
     3001 aaatcccatt ctacatctat atcaagtcga ttaccactag atgtttctaa taccatatat
     3061 ttgggaaatt ttatattttc tattttatca tctttactta cttttatttc atcactaaat
     3121 acaccaattc ccctatgaca acatttcaca atatcatagc cagaatcttc agctatagag
     3181 ggtataagct cattataatc atcaattata taaactttct tactttctga tataattgat
     3241 tgtgccaact tattattatc ttttagtaac ccactttcat tgttgctact atatgctttt
     3301 attgaagttt cttctatctc aacatcacct ttagaataaa cacctatagc atgttctcca
     3361 ttcacttcaa tatccttatt tagtattcta atatcatttt cagtataaat tcctattgca
     3421 tctttcccat ttgcctttat tttttcaaca gattcgtagg atgatttaat atgtaagtct
     3481 cctccttctt caacgtataa ggctgtccca tcagtagcaa ttatgttgtt tataccaata
     3541 cttatacttc caccattttc tatatgtatt actccttctt ttcctccttc acctattata
     3601 ttcaattcat tatcatcaat aataaatctc ccatgatttt ttatcaatat tttatatttg
     3661 ttggtatcta ttgtaaaatt actgtcataa ggaatataaa atctaaactc ttcatctatt
     3721 accatatcac aagaaagagt ataaactatt tcttctttat tatttttatt ttcttgaacc
     3781 cacctttcaa aatcttcaag acaattttcc tcttgtccaa aaactttatg tggaaaactt
     3841 ataaaacaca aaactattat actcataaaa ataaatatct catacttttt catcttacac
     3901 tcctagaaat tttttagctt tgaaacaatc tgtcgaaaca tacacttcac ccactataat
     3961 ttttccacaa ttatcaatta ttgtcaattt ataaaagtat ctttattaat tgttataaat
     4021 aaaaagctac ttactaaagt aaacagacgt ttcactttaa taagtagcta tatttttata
     4081 acaaaatagt tataatatta gaacttcctt catttacaat tttttgtaaa gtcttttgca
     4141 ttttaactct tatctcttct ggcatagtat ataatttact ttgtaattgc tcttttacta
     4201 agtcatgtag tgatttacca aacatatttg attcccataa atctgctggt tgctcttcaa
     4261 atacttccat aagatatttt atcatctctt caccttgatt ttgatttcca actattggag
     4321 aaacctctgt agatatatct gcttttataa tatgaagtga tggtgcattg gcttttaact
     4381 tgataccata ttgttttcct tgtttcatta tttctggttc ttccagacta agttcttcta
     4441 aggaaggagc tactactcca tatcctttta tcttggcatc tattaaagca ctttctattt
     4501 tatcatactc atttttaact ttagatagtc tagttatcag actaagcagt tgataatctc
     4561 cttcaatttt aaatccactt ttttcttcaa ggacattata gaataaatct tgctttgtct
     4621 gtaaatcaat atttataaca ccttctccta gctctacatt atcaacacct gtatcttcca
     4681 aaaactctaa ctcagaaaat ccttgtataa taccttctat atcccttatt ttacctatat
     4741 ctataatact ttgttttaat gttgttatta tgctactttt tatccaatga tttctttcaa
     4801 gaccttcaac ccattttggt aagttgattc ttatttctgt aagtggaaaa tcatataata
     4861 cagcttccat aacctcctct atatcttctt cttccatttc aaccacattc attggaagaa
     4921 ctggaacttc atacttttct tccaattcac ttctaagcat actcgtttcc tcactttttg
     4981 gacttaacgt atttaaaacc actgcaaatg gttttttaag atttttcaat tcttgtataa
     5041 ctctctcttc tggttctaca tagctttttc tgtcaattcc agtaactgaa ccatctgtca
     5101 agacaactat ccctattgta gaatggtctt ttataacttt tttagttcct atctctgctg
     5161 ccttttcaaa tgtcatagct tcttgtgacc aaggagtgga tactaatctt tgttttccac
     5221 cttcctcatg ccctaaagct ccttcaacta tgtatccaac acaatctacc attcttactt
     5281 ttaaagatac tgtgtctttt atttttattt ctacaccatc tgctggtaca aattttggtt
     5341 ctactgtcat tatcgtttta cctgaaccac tttggggtat ctcatctctt gttctatcct
     5401 ttttaaattc attgtctata ttaggtataa ccaacttttc cataaatttt cttataaaag
     5461 ttgattttcc tgttcttaca ggtccaacta ctccaatata tatatccccc tgagtccttt
     5521 tggatatatc ttcgtatatg ttattattca tattattcct cctatacatc taatcttatt
     5581 aaattatatt tagagttaag ggttaatatt cttaaaatag aaaaataaag accagcaata
     5641 ttaatttaat ttatatatag aaatttgtaa atttcaatgt aaattaaatt aatattgctg
     5701 gtccaaatag tagtgtatta tattaactga ctttagtagt attttcattt gaattattat
     5761 tcatctcaat aagttcttta cgtctttctt ttgtcatgtt catccatata aatgtaaaca
     5821 ccaatccaat tatggctact atagtaagaa ctatgaagta gtacctatat ccagttacac
     5881 ccggaaatgc atccaatatc ttccctgctg ctaaaggaca aagtacttct ggaagatatc
     5941 ctaacgtagc aactattcct atagcagttc ctgatattga taatgaataa ttaccttctt
     6001 caagtaacga atagtgtaaa ccttgaattg catacataga tacatatatt gcaacacatc
     6061 caatcaatag tatataaatc atagatgatt ttgttggtgt aaatattaca cctaaaagac
     6121 ctgcaatcat aagtataaat ccataagata taatctttga tgaaccaact ttatctccaa
     6181 gtaatccaga agcagctgat gctactggtc tacaatactg agctaatatt gagattgcag
     6241 atgcaaacac cacagtagct ccaaatgctt cagttgaata aggcgtaaaa taataaaatg
     6301 acatattagc tgaatatgaa caaaacatta ttattgttat tatccatgta tgaggcattt
     6361 ttattactgc aaaaaaggct tctttatcaa attttgctga tacctctttt tcattatcct
     6421 ttaatttgaa aaatactaat actccaacta ttacatttat tgctgagtat aatactataa
     6481 tagctgtaag accagattca tcacttactt ttgatgaaat atacccaaat agtatcaata
     6541 ctagagatag atgaattgca ttggtaattc cacgaccact ttccataaat ccaaaagctt
     6601 ttccttgttc attttcagat gctaacattc taacagcttt tacaagagct ggccaaaaag
     6661 taagtattgt tgttattccc cataaggcat ggattaaaaa cacaacaata tatggaggat
     6721 aaagtgttaa tacaagacca gatattcctg taataattaa agaacctgaa agcaattttc
     6781 tggctgaaat acggtctgct aaaaaaccac caaaaaggta tgataccatt gcaagtagac
     6841 cataaatgct tcctaagctt cccatttgtg tatttgtaag attaaatgct tgtgcaaaag
     6901 tatcataata ataattcttt aaatatggta aagcatatat aaatgctcct gctacaaaga
     6961 gtattatcaa gactttgaaa tttttccaaa tgcttggttt atttacttca actgacatac
     7021 ttcctcctta tgtaaatacc tgtaaatttt cataataatt aattattgct tattttattg
     7081 tttattcaat atttaggaat atttattaat atattaggtt aatctatcaa taatttatgg
     7141 tgagttatga gctaaaagta aactctttta gctcaccata ataattatat ttttatttat
     7201 actaacatcc agagttatta gatatctttt tttcatagtt cttccatagc attaaacctg
     7261 ttattataac aaaaagacct cctcctatca tttcaatctc atagattata ttatctttta
     7321 aagtctccat aactggtgcc gttgagaaaa gtatgccaac tacagttact acaagtacca
     7381 ttctggcaat attgatacaa actgaatcac tttttgcaat tgaatatggt ctttcttcat
     7441 ttggtcttgt ctttctaagt ctaatataag atgtaaaaag caagacatat ggaaataatg
     7501 ctgttaatgc tgtcatagta acaagaacat tgtatatatt atctactgat ggtaataagt
     7561 ttgttcctac aagaagtaga ctcactatta tagcctgaac tattactgca tttgatggta
     7621 tattgtattt atttgtaaca gtcaattgct taggaaatac accttctctt acatttccaa
     7681 aaagcatctt tactggacct gctatataaa gtattaatgc tcctagtact gataaagtta
     7741 ttccaaatgc tactattctt ataaaccaac ttcctatacc taaatcttgt gcaactttag
     7801 ctaatgcatc tagtatccct gtagatgctg caattttatc tggaggaagt atcattgtta
     7861 ttgctactga acctaagata tagattcctc ctattaaaac agccacagtt aatatagctt
     7921 taggaaagtt tttcttagca ttatccattt cggttatgaa attagcagtc gtttcagctc
     7981 ctgataatgc aaatattata gcagatatag aaactaatga attggcatct atttttggta
     8041 ttatattttg gattgtatat atcgatgctg atggagcttt tttgagaata actacagaca
     8101 taaatgccat cactataagt aatatagcag gaattgtaga acctaatgct cctgtattcg
     8161 taaaaaattt tccaaatgcc atcccacgag tacttactaa ggacaacagc caaaatatta
     8221 tgagtgaaag tgctaaaata aacatcttat tgtttgataa atctggattt ccaagcatat
     8281 atgcaatatt tatcgctaaa aaagtcaaga aagaagaata ccaaaatatc tttgatgtcc
     8341 aatttagcca tgacaccata aatccccact tctctccata agcttgttta acccattctc
     8401 caagtccacc atcttttgga tatgttgcag ccaactctgc acatataagt gatgcaggaa
     8461 caaagaatat aaaagcaaac aataaccaaa ctggtataga accaagaccc aatccaaaac
     8521 tagaggcagt agatttagct atccatctaa tcccaaatag tgctgatata ttcataaata
     8581 ctaaatccca aaatccaatt ttctttttca ctgtaattcc tccaatttca aatttattca
     8641 gactcataca caatgtattg acttaaatat caagtataag ttcttcaaca cttcttggat
     8701 aattagttat aattgtagca ccattcttac ctactattgc tgtatctgag tgtctaaatc
     8761 ctccaactcc aggtatatat atccctggct ccatagaaaa taccattcct tcttctaata
     8821 ttaattcatt gtcaaatctt aaatatggct cttcatgttc tgataaacct aaaccatgac
     8881 caattctatg atttgaatat aactctagac catactttgc aacaacagct cttgctgctt
     8941 catccaccat tcttgctggt ataccagctt ttattgtatc taatccagct tgttgagcct
     9001 ctactgcaat cttaaacact tctttttgtc tttctgttgg tttgcctata ataaaagttc
     9061 tttcattttc tgctctatag ttttcatacc aaacctgtct actatgtatt actatatctc
     9121 ctctttgaag tattctagta ttggaatcta aatgaggttg tgctgttcta tcaatccctg
     9181 aacaagtcca gtttgcaaat cctatatatg tatcaggata attttcagaa acatatttta
     9241 gtaaagcatt atcccctgca acgtcaaact ccaattcact catcccaact ctaacatttt
     9301 ctaaagaccc ttttattcct atatcagata ggtaacctgc tatcttcaaa aattctattt
     9361 cttttgcatc ttttacatat ctcatttcaa atattttact tccaacatct ttgatagcaa
     9421 atgacttatc agttatatac tctgaaaagc ttgcagatac tatatctttt tctattccaa
     9481 cagttgttcc tttagggtat ttatttaaaa ttatatctaa gtaatgttta tgtgatattc
     9541 catgttcttt catttctggt atttcataat acacatatac attatcaact tttgcaactt
     9601 ctttagcatg taattcttct aatccaggta ctattaattc tattttatct tgagttacta
     9661 ctgttacaat tggtctagaa taagttattg ctttaaatcc actaatataa aattgattgt
     9721 ctggttcaaa tattaaagca atttcaatgc ttttttcatt catgtacttt ctcaatcttt
     9781 ctagtctcat attatttgac ataatgcacc tctttcttaa tattcagata atttattcaa
     9841 tacaacaagg atgaatatat tattttaaaa ttcttaatat ttttagtact tgtcgtattt
     9901 gtatgtaaat taattttatt tgtgattatt ttatcataat catgattatt ttgtcaatca
     9961 tgattttatt ttaaaaaatt ttttatattc tagtgatttt aaattatcta aaatataacg
    10021 agtatacatt gtattttgta atattattct tgatttatgg taagtttaat gataaaatca
    10081 cgattatata aactatattg attatttgta ggtgaataaa atgacaaata aagaattaca
    10141 aaagaaaagg atattgatgt atttcataga agctgctcaa aatattatgg aaaacgaagg
    10201 tatagaaaat attacacttc gtaaggttgc agatatggct ggctataata gttcaactct
    10261 atataaatac tttaaaaatt tagaccattt aatatcattt gcatctataa aatattttaa
    10321 agattataac ttgaatattt caagatgtat agaaaatgtc aatgatgaat ataagaaata
    10381 tataattatg tggaaattat tttgtaagca ttcttttgat aatgctcaag ctttttacca
    10441 ggtatttttt aacttgtcta gtgatgaact tagctatata acaaaacaat attatgatat
    10501 gtttccagaa gatttaggta ttcatgatag tgacatatct ttaatgatta caggagaaag
    10561 tataaaggaa agaaataaga tactattaaa caatcttgta gatactggat atataccagc
    10621 taaagattta gacataatca atgatataat tatttctttt tatcaaaatc tattattctt
    10681 aaaaaagaac acaggaaaaa attctgatga tgatgaatta actatgaaaa tgatttcttc
    10741 tattgaatac ataataaaac atgctaaata aaattaaaat atggctgtct caaaatagag
    10801 ataagccttt tttattgttg aaataataaa aatatattta atttcatata aaaaagagtg
    10861 cctcgtaaac taaaaagttc attttgagac acccctaaaa taaatttatt atttattata
    10921 caatagttta tatatcatca cctaaaacta cttcttccat ttcatgcttt ttacttctca
    10981 tcattagttc tattccaact tctttaggat ttgaaccttt ctttattaca gaatatatag
    11041 cattagttat aggcatatct atatctaatt tttcagcaac atcatgtgct acttcagtag
    11101 cagttatgcc ttctacaacc atctttactt cttttaaagt atcttctaaa ctcatccctt
    11161 tacctatgag tataccagct cttctatttc tactatgcat acttgtacaa gtaactatta
    11221 aatctccaat acctgaaaga cctgcaaagg tagacatatt agctcccata gcaattccaa
    11281 gtctgctcat ttcactaata cctcttgtca taagggcagc ttttgcatta tcaccatacc
    11341 ctaatccgtc acatatccca gctccaaatg ctataatatt ttttaatgct cctccaagtt
    11401 ctactccaac tatgtctgga ttcgtataaa ctctaagttt agggctcata aataaatctt
    11461 gtatcatctg tgctattttt aaatctttag aagcaactac tacggtagta ggaatatccc
    11521 ttgctacttc ttcagcatga gatggcccag atagtattac atatggattt tgtggtaatt
    11581 cttcttcaca gacttgagat aatctaagcc cagtcccttt ttcaagacct ttagctacat
    11641 taacaataac ttgtccttct tttataaatg gttttatttt ttggcaagta cttcttacag
    11701 cctgtgatgg aactgctaaa actattacct tacaatcttt tatagttttt tctatatcag
    11761 tacttatagt tatattattt ggaaataata ctcctggtaa atagtctgta ttttctttag
    11821 ttctgtttat ttttttagct tgttcctctt ttcgagtcca catgctcaca tcattacctt
    11881 tttttgcaag ccctaaagct aatgcacttc cccagcttcc agtacctaat acacatactt
    11941 tctccatgtt taacaccacc tgagtataat atatttacta ttctttcttt tgaccaattt
    12001 ttctctctgt cccatttaat aatcgcttta tatttgctct gtgattatat attactgatg
    12061 cagttaagaa cagagctact attaaaccag ctttattttt taccataatc ataaatattg
    12121 gtgaacatcc tattcctaca acagaaccca atgaaacata ctttgttata gctaccacca
    12181 atataaatac tgctagacac attagtgtta ttactggatt tacagctagc attgccccta
    12241 gtgaagttgc aacacctttg cctcctctaa atcctaaaac cgctggccaa ttatgacctg
    12301 ctacaacaca tataactgcc agatatccag ctaaaagagt atctattcca accaatcttg
    12361 ctgcaaattc tgatattaaa acagccatta cacctttaag aacatcacct aaaaatgtca
    12421 ttgctcctgc tctttttcca agtgtcctaa gtacatttgt cgaacctgca ttaccagaac
    12481 cttgagtcct tatatcaacc ccagcaattc gctttgcaac tatgtatgag gtagatatat
    12541 ttcctaaaag atatgcaact acagcaataa ttatataact aaatatctcc actgaatttc
    12601 ccccctttag ttatatttta agatatttat tttttatttt tttgtctata ctcaaacttt
    12661 atggaagttc cttcaaatcc aaaattttct cttatcttat tctcaaggta tctttgatat
    12721 gagaagtgag ttaaatcctt atcatttata aacaatgtaa ttttaggagg tctaatgtca
    12781 gtttgagtcc catagtaaat ttttaatctt ctacctttgt cagatggtgg ttgatttaac
    12841 attacagctt ctccaattac ttcattcagt gctgaagttg atattctctt agaatgttca
    12901 tttgacacat attcaactgt atctagtatc ttgttcattc tttgattagt ttttgcagaa
    12961 acaaatacga ttggagcata catcataaat ggaaattttt cttttatatc ttttgtgtaa
    13021 ttgctcatag tcttattatc tttctctatt aaatcccatt tattgattac aaatatgcat
    13081 ccttttcctt catcatgtgc tatacctgca acctttgtat cttgttctgt tacaccttct
    13141 aaggcatcta ttactatcaa tactacatct gctctatcta cagcactcat agcccttatt
    13201 acactgtatt tttctattgt ctcatatatt ttactttttc ttctaagccc tgctgtatct
    13261 attagaagaa atttctgtcc atttttttca aaataagtat caattgcgtc tctagtagtt
    13321 cctgcaattg gacttacaat tactctttct tctccaagta tcttattgag tatagaactt
    13381 tttcctgcat ttggtttacc tgttatagct actcttatta tatcttcttc atactctgta
    13441 tctaatcctg ctggaaaatt ttcaactatc tcatctaata aatctccaaa tcccatacta
    13501 ttggctcctg aaactgcaaa aggcgttcca aatcccaact cataaaaatc gtatatatta
    13561 tcaaattgac tttgactatc aatcttatta accactaaga ttactggttt ttttgtcttt
    13621 ctaagcaatt gtgcaatttc tttatcagca gctgttattc cagctttacc atcaactata
    13681 aaaagtatta catgtgacat gtccattgca agcatggctt gatttctcat ttgagataat
    13741 attatatctt cgctatcagg ttcaattcct cctgtatcca ctaatgtaaa atatttatct
    13801 aaccattcta cttcagcaaa tattctatct cttgtaactc caggtgtatt ttcaactatt
    13861 gatatccttt ttcctgcaaa tttattaaat attgtagact taccaacgtt tggtctccca
    13921 actacagcta caactggcct acttatactc attttatttc tccttccatc ttttgttttc
    13981 tattttaaaa ttttatccac aaaatctttt ccttcattca aacaagggat aatttttatt
    14041 ctcataagtt cttctaattg atttaattca atattatcta agaaaatttc ttcatctgct
    14101 tttagcatac ttcttgttat ataaagagct tctcctaatt ctttatcttc aagttgttct
    14161 tttaaatcct tagctgttaa aagacctgat acagttattg tctctccaaa aaacttgttt
    14221 ttgatttcat atacatttac ttgaagattt ttaaacttgt ccatcatagc atctgccata
    14281 ctctgtataa attcatatgc agaatgacca gtagctattg atactttctt ctctcttttt
    14341 agaatcttct caggcaatat atttagatac tcttcaatct ctgtttttaa cttacgtatc
    14401 atacctacac catcttcaaa ctgtaaaaaa ccttcatatt cttcatatcc aggcaattca
    14461 ctattagata gtatataaaa ttcatctgat aaaaatgcaa atcgagtacc taatttttca
    14521 agatatttct gttgtatttg atgtatttgc tctatagttt tacttgcgct cttatcatta
    14581 aatatattta attctactag attttctcta tgctttgtaa tcccaactgg aactatcgca
    14641 acactgttta catacggata aagttgagct agttctttta ttgttctatc caattccttt
    14701 ccatcattta tacctggaca taaaacaatt tgacagttca tttctatatg agcatctgcc
    14761 aatctcttca ttataccata taattttcct gcaaatttat tgcttatcat tttttgtctt
    14821 aactctggat tagttgtatg cacagatata tttataggac ttattctata ttttataata
    14881 ttatttatat cttcttcact catatttgta agtgtgacaa aatttccttg taaaaaagat
    14941 aatctcgaat catcatcttt aaaataaaga gtttctctca taccttctgg caattggtct
    15001 ataaaacaaa acatacactt atttctacag cttttagctt ggtcaattat cggatttgta
    15061 aattcaacac ccaaatcttc atcatagtct ttttctatct cgtatatgta gacttcgcca
    15121 tctttttttt gtatttctac atctaaatat tcatcactta gtaagaatct atactctatt
    15181 atatcatgta ttggctgttc attcacagat ataagtaaat ctcctacctc tatacccatt
    15241 tcttctgcaa tactatcttt ataaacctta cttataatat tatttacttt ttttacattt
    15301 acttccaata ttttcaccac tcttttgtat tcatcaatgc ttattataat gtattttaat
    15361 atgcaattta tttatttatt atattttatg atagcatttt taagcatttc atgtcataat
    15421 aacacatttt aattctaata gtcaatataa gtaaatagct ataaaataga ttagaacaaa
    15481 taatattcaa atttaagttt cttaaaaatt taaatattat ttgttcttaa aaaaatttta
    15541 taaatttaat gttttactat gattaacgtc ccatttgtca tatcatgaac cattccacct
    15601 acacactttg acatcaacaa tgccttattt tgtaagtctt tctcatcatt tgcataaaat
    15661 attggacaac ctccaccatt tttcatattc ttatcaagtg taactatagc tagggtatat
    15721 tcatttaaac ctatatccat tcccatctca tgcacttcct ttctttccta ttttataatt
    15781 ttttagtgat aagttttttc ctttagagct tgaaagtatt gggcatgact ttacagcttc
    15841 cattactttt tccacattct tatctactgg taaataagca atcattatac tttcatcctc
    15901 tttatttctt ctaggtaatg gtacgaatga aggttctcca taatctccat ataaacctaa
    15961 ccttgaatat atattgtata ctattgcttg tctttgaccg gagtcaaata atattcctgc
    16021 attagcataa tccttatttt taggaattat ttgtattcct acacctctat taatatatac
    16081 ttctctatcg ctttttagac ctatatttgt tatacctttt aaatctccca cctttagtat
    16141 agattcgtcc acaaaactaa tttcagcagg cattacatca gctatgtctc caatactttt
    16201 tctagtaaga agttttttta atataaaggc taacactaat ccacaaattg aacttattac
    16261 caaactcatc ccttcactta ttttcgtttc tgcaagtatt acataataca atcctattgt
    16321 taaaaaagat gtaactatac acatataatt tcttacttca taggttctag ctatctcttc
    16381 tatgaatgca gctcctctct ttacaagttg aacttcctct aaattttgca atgtatttct
    16441 tctatttgct ctcacttgtc taaattgttc tgctgctaga gataaaaaag ttatagatgt
    16501 ataagaacga tttataagag ctggaaccaa taatgctcct aatgaagaag cgacgaatgc
    16561 taatataagt tgagatagta aaacatttgg ttgcgttgga tactgttttt ggtctaaatt
    16621 caacattata attctagatg ttataccaat aataacagca acaaaaaata ctctatccat
    16681 taaatcactc ctaaaatatt taatttgaaa ttttacatgt aaatctacaa agtctttcta
    16741 gaaaaatcac tctgctttcc ttttgatgtt tctatgatag gtgtactttt cactacatct
    16801 atcatgacat ctatatcttt gagtattggc atatatggta taaccacagt acttttttct
    16861 aaattggtct tagatatagc aacaatatca acctcatcta catctctatc tatccccata
    16921 tgaataaata gattatgtat tatagcttgt ctttgcccta tatcatttac tattccaaat
    16981 gcacctaaat ctcttggtat aacttctatg gccagacatt catttctata cttttctctt
    17041 gtactttcta gacctatgtt agttattatt acaccattta ccattaaaat aggtccttca
    17101 aaacttattt ttgcaggaac tatatctgct atatctgcta tactgttcct tcttaaaaat
    17161 cttctaaaaa tcaaaccaac aatagctcct gacacaatag ctaggattgt gcaataaaaa
    17221 aagctaaggc catactttct tgcaaaaacg atataaacta tcgaagatac cagtgcagaa
    17281 aaaagactta tataacttct agattcatac gtagatgcta tttcttcaac gtaagcgtct
    17341 ccttttggta ctaattcttc attatcgata ttctttagtg ttattctttc ttgttctgca
    17401 agaccttgaa actgttgtat tgctactgca aaaaatgtca aggctgcaaa ctccttatct
    17461 ataagtgcag gtaatgctat agcacctaac gaagctgaaa gcccagatat aattatctgt
    17521 tctaaataat cttgtggtct tgatgggtat tgtttgtctg taactcttaa aactaatcct
    17581 ctacataata ttcctacaac taatgcaact ataaaagagt gtctaaataa atttgcattc
    17641 aataaacttt cttccatttt ctcagctcct tctcttatat agtttgtgca aaaaacaaaa
    17701 aaaattccct tctatttaag gaaatttttt tgtaatattg taactataaa ttcgctacca
    17761 atgccaagtt cactttttac ttttatacat ccatttagtg atttgactat atgctttgtt
    17821 atagccaatc ctaaacccgt tcctcctaca tccctacttc ttgctttatc taccctataa
    17881 aatcgttcaa atatgcgttc tacatcttct tttggtatcc ctacaccatt gtctataact
    17941 ttaattacga ttttttcttc atcttttaac actacaactt gtacaatccc attatcttgg
    18001 gtatacttta tagcattatc aactaggttt aagaacactt gctttatata gtctttattg
    18061 gatagtatac ttattgattt atcatcacat ttatataaaa cttctatatt tttttcacta
    18121 gctgaatgtt gagttatttc atatacctct ttaaatacat catatactac aacattttcc
    18181 ataagaagag tatctttatt ttctacaaat gataacagca atatatcatc aatcaacctt
    18241 tttaatctat ctgattcgct ttctattatt tctaagaatc tatttctagt attcacatct
    18301 atattttcat ttgactttag tgtttcaaca aacccactta tagatgtaag tggtgtttta
    18361 agttcatgac ttacatttgc aacaaaatca cttctcatat tttcaagttt aactttttta
    18421 gtaatatctt ctatatttac tatcgaacct attattacgt tgttagtatt ctgaagataa
    18481 actgggtcta cttttatctt atatacgata tcatctttag tagtaatatt agcagtttca
    18541 ttatctttag aaccaataaa ttgaagtatt tgttttaata tttcttcttc ttttattaca
    18601 acattaatat tatgaccttc aactaattga tttggttcac tctttatcat ttcttttgct
    18661 tcatcattta tcagaagtat atttccattt acatctatag ccaaaattcc atgagatata
    18721 ctctttaata tagaccttaa ctgtaaatgt ttatactgta cttcttctaa ggttttatcc
    18781 atattcttta tcatatgatt aaaatttcta gctaattcgc caagttctcc tttggctgat
    18841 atgttaagcc tagcatgata ttcattatta cttacttttt ttgagacgta tataaactct
    18901 tttaaatatt tccttaatgt tattgtgtat cttacagcta atataacaat ggctatggac
    18961 aacataacaa taaaaaaata tatactattt aaatcttcca caagataaca ctcctaaaat
    19021 ataacctttt taatctattt tgtatcctac acctcttata gtctctatat atttttccga
    19081 tttatcttca tcttctattt tttttcttaa atatcttata tgaacatcta ctgttctagt
    19141 ttctccatag tattcatacc cccatatttt atccaaaaga taatttcttg aaagtacttt
    19201 tcctttattt tgaattaata attttaacaa ttcaaattcc ttaagagtta actctatctt
    19261 ttcagagcct ttggtaactt catgttttga taaatctagt tttaaattac ctgtagtaag
    19321 tatattgttt tcttcaccta agctagtcaa attatatctc cttaaaacag aactaatcct
    19381 agctaacaac tcttttatac taaatggctt agttatatag tcatcagcac ctatttcaag
    19441 cccttcaacc ttgtcatttt ccatattttt agcagtaagc attataacag gaatattttt
    19501 taagtcctta tcacttctta tttttctcaa aacttctatt ccacttatat ttggtaacat
    19561 ccaatctaaa agtattaaat ctggtttaat ttccttagcc tttataaatc cgtcaaatcc
    19621 atcatatgaa taactaactt cgtaatttga cacctccaaa ttgaatttta atagctcaac
    19681 tatatgcatt tcatcgtcta taacaagcac tttagtattc attaaaaagc tcctctctat
    19741 tttatctgta gtaccgttcc tattcgttct gatgccttat tatgttttct ataagagtgg
    19801 aatttatctg ctctacaact agtgcataaa tttaaattta ttatattttc ttttttaact
    19861 ccactacatc taagcatata ctcattaatt ttccataaat ctaaatagta acttccctct
    19921 tttattatat agaacttttc atcattattt gtaagaattg tgttaaattt ttctactaaa
    19981 tcttcagaca cttcataaca acatggtcca attgatggcc caattataca tactaaatct
    20041 tcaggaactg ttttatagca ttcagacatt aattttattg ttttatgacc tatattacta
    20101 aaagtccctc tccaacctgc atgagccagt cctatggctt tatttttagc atctataatt
    20161 gatataggta cacagtctgc tgtaaacagt aaaagtggta ctttttccaa gtttgttata
    20221 agtgcatctc catctcttct tttccctata gtatctttat tgactatatt aactatatct
    20281 gaatgtattt gagaattact agttagattt tcaaatgtca aatcacattc tttacatact
    20341 tttaacatat cttctctatt ttttgcatct atattcttac atgttataac tagatttaca
    20401 gaatttaatt tttcatcaag ttcttcacaa ttaccactta aattaacata ctctttaata
    20461 tcttgtacat tttttatatt tatataatct ttcatcattt gtctcctttt tctatcagta
    20521 tacttttaag ttcatttaca aaagtattta tatctttaaa ttgtctatac acagaagcaa
    20581 atcttacata ggcaacctca tctaactctt tcaatttatc cattaccatc tcaccgattt
    20641 ttttggtttc aatttcttct ataaaacatt tatttatttc attttcaata tgtacaacaa
    20701 tactttcaat ctcgtcaata gaaacaggtc ttttttcaca tgcttttagc aatccatatt
    20761 ttatcttttc tctatcaaaa tattctctac tattatcctt tttaatgacc attaaaggag
    20821 ttgtttctat tttctcatac gtagtaaacc tctttttaca tgattcacac tcacgtcttc
    20881 ttctgataga ttttaaatct gtatgtctag aatctattac ctttgattct ttataattac
    20941 aataaggaca ttgcatctat attcctcctt tgtttaacta tatctcaaac tcatctaagc
    21001 ttaattctga acctatattt actattattg tatcacaacc tattttaagt atatcattcc
    21061 aaaatataac ttcttcatcc cctcctcttg aaaaaaaact tctacttcca tctccaaaaa
    21121 tggtaaatga gactacctta ccttcgtgaa tatccatgtc tatgtctatt ataaacccca
    21181 tcctttttcc attttttaca tttataattt ctttttccat tatatcagaa actctaatca
    21241 tattgtactc accctttctt taaaaataaa tatgtatcga agtaaaaaaa aatgacctat
    21301 ataggtcatt taaattaaaa taatgtttac agtccatgga ttacttatac atattttctc
    21361 atatttttta aagcattttt ttcaattctt gatacttgtg cttgagatat accgatttca
    21421 tcagcaactt ctatctgagt tcttcctttg taaaacctta aatctaatac caacttttct
    21481 ctactattta atttcttaat agcttcttta agtgatattt cttgcaacca attttcatca
    21541 gtatcttttt tatcttgaac ctgatccata acgaatattg catctccatt gtcttgatat
    21601 actgggtcaa acagagatat tgggtcttgt atggcatcaa gtgccattac aactgattct
    21661 acttctaatt ctaattcctt tgctatttct gatactgtag gttctttaga atttgttcta
    21721 attaatcttt ctctaacttg tagtgcctta tatgctatat cttttaatga cctacttact
    21781 ctaattgggt tattgtctct tagatacctt ctaatttctc ctataatcat aggaacagca
    21841 taagttgaaa accttacatt ttggcttaaa tcaaagttat ctatagcttt tataagacca
    21901 atacaaccta tttgaaataa atcgtctata ttttctcctc tgttgttaaa cttttttatt
    21961 acactcaaga ctaatcttaa attccctctt acaaactgtt gtctggcttc ttcatcacca
    22021 ttttttattt gtaataaaag ttccttcatt tgcttatttt taagaactgg aagttctgat
    22081 gtattaacac cacatatttc aaccttatta acttgcatat attttcagtc ctctctcaaa
    22141 agtattcatc tattgaaatt atttacattt ccaatttttt tatacttttt aagaagaatt
    22201 aatttagcat tatacaaatt ttttcatttc tttttgcaac cttgagatta ttttcttttc
    22261 aagtctagat atgtaggact gtgatattcc aagcattcca gcaacttctt tttgtgtttt
    22321 ttcaatacct ttatcaataa gaccaaatct taattccata atttgctttt ctctgtcact
    22381 taatctgtca agtgccataa ccaacaaatc tctatctatt tcttcttcaa ttattttgta
    22441 tatttcatca ttttctgtac ccaatacatc agataataaa agttcgtttc catctaaatc
    22501 tatatttaaa ggttcatcaa atgatacttc tgttttcttt ttattatttt ttcttaagta
    22561 cattaatatt tcattttcta tacatctcga agcataagtt gccaatttta tatttttatt
    22621 cagtttaaaa gtattaactg cttttatcaa acctatagtt cctattgaaa tcaaatcttc
    22681 tacatctata cctgtatttt caaattttct cgatatgtag actactaatc taagatttct
    22741 ttctattaaa attgacttta cactttcatc tgtttctagc ttttgtaaaa gctccatttc
    22801 ttcttctggt ttcaatggag gaggaagtat atttgcccct cccatatagt atattccctt
    22861 tggctttatt atttttaatt taatacctaa catagtaata aagcttatta ttctttcttt
    22921 caatcgtaac attccttgtc ccccttaaat tttcacctaa aatccaggca atatatttgg
    22981 attcattatt gctccatatt ctctatcatt aaaatttgaa ataccaagta ttatatttcc
    23041 tatcttttgc tcatctactt ctatatagtc agcttttaag cctattatta tgcttgtctt
    23101 attacttcca gcatgtttat aaggtattat tctaactcta cctgaagttt tatcatctaa
    23161 acttcctata atttcttgtg ctttcttcat atctatatct tcataattaa tatttaatag
    23221 tttttctgga ataaatcctt ctaacacact agacttaact attattacat cactttgact
    23281 tattgggtct tttaacaaat taccactatc tactaaagcc ttacagcaaa aacttttttc
    23341 tagtaagttt atatttatag tctttgtaag ctccttaatg tacttaagca ttttcaaatc
    23401 tttataaatg tattttaata attcacaact cacataagta catgcaatta agaaagatat
    23461 ttttaagtga cttattcctg taaaataaat tataaaataa gtgcttccag aaataaaaat
    23521 gtttactaga taaaaaacta acgctacatg aacataatct tttttgctct tacttctaaa
    23581 tgaaattaaa gttattatac tcatgattat tattttaaat ggtacagtaa atagtatatt
    23641 cattgatgga tacaaataag caacagaata ggcacctcct aaactagccc ccaataactt
    23701 atttttttta ggattatatc ttttagtaag aatagaagta caacttataa taatataatt
    23761 tattactagg ttttctataa catagtactc aatatacacc atgtatcccc ccttaaaacc
    23821 tttaaacata ttataagata gtatgtacaa aattttatgt cacttttgat ggtcgaatcc
    23881 aaaacaaatt ctatttaaaa agaacaatga tatatgtctt atactttatg tcgtcaaaag
    23941 atattttttt aataccaaac aaatagactt ttttgtagaa tccaatatat ctaggtagaa
    24001 gttattcact ctacttttgg tatatttaca aaaaaagact acataattta ataatatatc
    24061 aagtaaatct tttacttata taaaatcaaa ttatatagtc tgtaaattca aatataaatt
    24121 attgtgctag taatccattt gcatattttg cagattcatt tagtgcttgt tctggagtta
    24181 tgtctccagc tatcatcatt tgaagttgtt tccataaata ttcatatatc tctactccat
    24241 gaggtccaac tgtcaatggt ctatacatat gtttatctct tgtcaccatt tcttcaaatc
    24301 ttgggtctcc ttggtatttt tcacctttag caactggagc tctaggtatt tctttgtgga
    24361 aagcttccat tgattctttg cttagcatat actcaagaag tttaaatcca agttctttat
    24421 ttttacatgc tgacattaat gttaaatcat ctactgaagc aaaacttcca actgtcttat
    24481 cttctaatga tgttacatat ccccagttta aatccgggaa tgatttatca aaagtacttg
    24541 tagcagcaga ggatagcatt atagtaaatg cagcttttcc tggtccaaat acactttcaa
    24601 tcatttcatt actatctttt gataaagcat cttctggaat atactttttc atatcattta
    24661 taaagtttgc agctttcaag ccttctgcat tattaaattt aactgatttt aaatcatcat
    24721 tatatatatc tccaccagct tgccataaat acggatacca gttccagttt aagcttccaa
    24781 atactttaga accccaacct tgtgcaagac cccactggtc gatttttcca tctccatcag
    24841 tatcttttgt tgctttttca cagattcttt taaaatcatc ccatgttttt ggaggttttt
    24901 ctcctaattt ttctagtata tctttattgt agtacaatac gcctggatta gcagcttcta
    24961 taggtaaacc ataaatacca cccatcattt ttgcatcatc taagtataga taattatctg
    25021 aagttcctga tttttctaaa taaggtgtta aatcttctac cgcacccatt tctataaatt
    25081 gaggaaacat ctctgcatac atgtatccta tatctggacc ttcacctgca cttattgctg
    25141 ttgcatattt ttctgcgtag ttatcccatg gtactatctg aaattcaatt tcacagttat
    25201 tttctttttc aaatttttca aatatcggct tccatgcatc tgcatcatgg tctgttaatg
    25261 gtgcagacca tacaactaat ttttctttac cctctttgcc tttagaatct tctttttttg
    25321 aacatcctgc aagaattgat actgatagaa tccctaccat aactaaagaa agaacctttt
    25381 ttagtttaat catgcttaat cccctttcag actttaattt gtctttttcc tcaaatgttt
    25441 acatttaatt cagatttaat attaacaaat aagattactc ttgtctaatc ataaaaaact
    25501 atactaactc ctataactat attttttttc tatacctatt agatttaatt catactaaaa
    25561 acttactttt taaattttat caatatttaa aaattttttt aagtctagcc acaaactaac
    25621 tatctaaatt gagcaaaata aaaagaagtc tattgacttc tttataagct tacatattat
    25681 cttcttcttc ttaggaatgt aggtatctcc atgctctcat catcatcttc ttcaattata
    25741 cttgcctttt taggaggctc tattttttcc tcagcaatta cttcttcttc aatttctttt
    25801 acagcttgtt ttactgttgt atttaatgaa gaacgtatgc ttgatttagg tttagtatct
    25861 aaatctaagg caccattttg taatccttca aatcctgtcg cgattactgt aatcattatt
    25921 tcatcaccta aatcttcttt tatagaagct ccaaatataa catttgcttc tgggtcacag
    25981 gattccataa ctaaagttga tgcctcatta acttcaaata atcctaagtt aggaccacct
    26041 gtaacattaa gaagtactcc tttagctcct tgtattgatg tttctagaag tggagattga
    26101 atagcttgtc ttgcagcatc aattgctcta gtttcaccac tagcactacc tatacccata
    26161 tgagcaagtc ccttgtcttt cattatagta gtaacatctg caaagtccaa gtttattaac
    26221 ccttcaactg ctataaggtc tgaaattgat tgtatacctt gttttaatac atcatctgca
    26281 actgcaaaag catctaacat tgatgtattt ttttgtacta tctgtaaaag tctatcattt
    26341 ggtatcgtta taagagtatc tacttttgat ttaagttctg ctattccacc ttcagcgttc
    26401 ttcattctta tcttaccttc aaatgcaaaa ggtttggtaa ctacacctac tgtaagtatc
    26461 cccatttctt ttgccaagcc tgcaaccact ggagcagctc ctgtaccagt accaccgccc
    26521 attccggctg ttacaaagac catatcagct ccttgaagta actttactat ttcatcttta
    26581 ctctcctcag cagctctttt ccctacttct ggatttgctc ctgctccaag tcctctagtt
    26641 aatttttctc ctatttgaac tttatattca gcttttgatg tatataaagc ttgtttatct
    26701 gtatttactg atataaattc aacgcctttt agttgagctt ctaccattct attgacagca
    26761 ttgtttccac cgccacctac acctattact ttaatttgtg cacattcttc taattctacg
    26821 tcaaagttta gcatcataaa cccccttctc tcatgattca aagttatcgt atgtattaat
    26881 tatatcattt tcttctttta ttttgttaac ctttaagtaa catattccac aaaaacgtaa
    26941 attttccttt ttaaatttct tacttatatt gtcaaatatt actctttaag tataaaataa
    27001 tttacgtata tattaaatgc tctttaattt ttacgttttt tgttcataat attactaata
    27061 atatatcgtc taattataga taagttatta aacaatctca taccaaaaac taatacagca
    27121 acatagtaaa gagaaactcc taacttttct cctatataag tcaataatat agccaatata
    27181 gcattaccta tgaaaccagt aataaaaatt acattatcat agttatcttc aaaattggat
    27241 tttatcgctc caaaaataga atccattatt gcaagaatag ctactgacat atatatggaa
    27301 taatccatag aatatgtaaa tggaatatag taacctacta ctacaccaaa tgctaatcct
    27361 cctaaaaccc ataacataaa tcttcacctc tttattttac tttgaaacac aactacatta
    27421 caatcctttt tattaatttt ttttactttt tctttatttt tttgtatttt tcgttttatt
    27481 ttgcaaataa atccacttta tttccattat tttcctgaac ttttatatta attccatata
    27541 tatccttaag agatttagca tatgattctg gagagtttat agctgcactc aataaatcta
    27601 tatctcctat agcttttatt ataaatggtt gtccatatgt agtatcattt actgtaatag
    27661 tagctcctga acattttatc ttacttgttg ctagtactct ttctccattt atagaaattg
    27721 ctcttgctcc agcctttttt aaatcattta aaaccctcaa aatatctata tcatgtataa
    27781 tcaaatcgtt tggattctgt ccgtctttta actctctctc actatccctc atagtaatag
    27841 ttatgcctgg ccctgtaaca gttgaataac cacttaattc tttaacttct tcaagctctt
    27901 tttgcataag tgtttggata gattcatttc cattcttttt tgcttcttta tattcattca
    27961 aagttttttt gttgttttta agagtttctt ttaaattttt tatttctttt tttgtatttt
    28021 ctacctcact ttcaatactt tcagtttgac ctaatgttat gtatactcta tttgggtcta
    28081 gtgattttat ataagttgat atgaaaacgc ctaatatgaa tgctcctaat ataactattt
    28141 tctttttcat actattattt cccctctgct ttgtttatat cactatgacc atttatttct
    28201 atattgtctt ctttggtcac ttttactttg gcattataat atgtttctat ttcccagaca
    28261 ataccatact ttatattaag tgccgaagcc aatgtatcag aatcaccaat tgcttttatt
    28321 ataattggtt cttttatgac agtgtcattc atataaagct tgtcttcttt taaatacata
    28381 tatgtattag aaactattct ctggtcgttt atagatatgg cacttgcttg tgctgaattt
    28441 aatttattta tcattgacaa taataaatca taattatata gtatactatc atttttatca
    28501 gagtttccta agtcttttgt agaatttatc tcaattttta tgcctgttcc ttcaacatct
    28561 gtatatccag ctaatgtttc atatttttct atttctgact taagtatgtc ttgttctcct
    28621 tcattgtttt tgcctctata agtctctata tctttcttca cactattaac ttctgattgt
    28681 agattctctt tttctttttt taaagattta agttcttctg taagttgctc tcctctttta
    28741 aaagttgtca tccctctatt ttctatatta ataattttaa gttgtaaact aacaagaaca
    28801 cccaatacaa tacttcctat gattatactt ttatttataa atcttttttt caatatatca
    28861 cttccttaca attgtctatc cttctggact atacactgcc aactttccat agttaagatt
    28921 tatagtccct ccattagtat ttttattttg caggtcaatc agaattttac ttaccctaga
    28981 tatattataa tctaagttat catcattaga aagtaagatt tttatattac ttcttgtaag
    29041 catttctata ttgctttctt tttttaagtt tacataattt atttttctat aaattccttc
    29101 ttcttttaaa taatttaaag ttttaaatac attctcctta gttttataat ttttaaattt
    29161 tatagattta ttatcatcaa tgctataatc tacatctact atcaagtctt ttttactttc
    29221 attagaacct cttagttctt ccaataaatt tcctttttta tctatataac aataattatc
    29281 ttcatctttt aatactgcaa atatttcttt ttcttttaaa cttataatta tcttattggg
    29341 aagttttctt tttatttcca cattttctat atatggattt tttataagtt tatttttcat
    29401 atctttaaaa ttgtatgcaa aaatattttc atttagattt acatttagtt ctttcattat
    29461 atttgatttg gtcaccctct tatttccaat tacatctatt ttctttacat caaataaatc
    29521 actttttaca attatacata tacaaaaaga taacataagt ataaaaacta aaactatcat
    29581 gacattattc gtatttaact ttcttctttt tttcaaatat aatcaacccc tttggttaat
    29641 aaatacacct ttagagcttg tttttcctac aaaaaaatta taatatttaa agtcaaattt
    29701 ttgcatttta ttacataaca tccaaataag gtattaaatt ttataaataa atcaatattt
    29761 catcaaaata aacatagcat actacatact atttatatat ttaaatctgt atacatacaa
    29821 ataaacccct aatttataat aatttagaaa atctaatata ttataaatta ggggtaaata
    29881 tttacttatc ttttctttat tcctattacc ttagcttgac cttctatact tggagtcgtt
    29941 tcttttttta tttcttttac ttccttaata actttttctt tttttgtttt tttagaagtt
    30001 gacttttgtg ttgaattgta tactttcatt atctcatcat atataaggtc tatggcttca
    30061 ggcttaccta tagttttact tgcatttgcc atatcaacca aaagttctct atctcctaaa
    30121 agtttaaata ctgctgtatt taggctctct ggagttagat ttttttctaa aatagcaatt
    30181 cctgcccctt gtttttctat actcttggca ttatactctt gatgattttc agcagtataa
    30241 gctttaggta ttattataga tggctttcca agagccgtta tctcagctaa tgaaattgct
    30301 cctgcgctac ctataaccaa atcacttgct gcaagtgcat ttgccatatc ttctaagtaa
    30361 ggcacaactt tttggtatgg tttaagattt atatcgctaa tacttcccat aaattcatca
    30421 taatagcttt tgcctgtagc aaatataaaa gctatatctt catttaccat attctttatt
    30481 acaagtctca ttgcatcatt tatttttctt gaacctccac taccaccata gcaaagtacc
    30541 attctttttt cgtcacttat tgatagattc tttcttgcaa tattttttct tgataataaa
    30601 atttcttttc taactggatt tcctgtaaaa actaatttat cttctgctgc ctctggaaat
    30661 cttttatgcg aatcttcaaa acttgtaagt acctttgtaa ctgttttaga taaaatctta
    30721 ttagttacac caggaaaaga attttgctca tgtattatgg ctggtatttt ccccatagat
    30781 gcattaaata aaacaggtcc actaacatat ccaccagttc ctataactat atctggttta
    30841 aatttcttaa caatctttct tgattgctcc aaacctttaa aaagcttaaa aactcttttt
    30901 acattatcga aatctatttt tcgcttaaaa ccttgaactg ttacagtctt taattcaaat
    30961 ccatacttag gaactatttc tgactctatt cccttttccg tcccaacaaa aattatctca
    31021 gcgtctggat gctcatctct tattttgttt gcaatagcta tagctggata cacgtgccct
    31081 ccagttccac cgcctgataa taaaactttc atcaaatcat catctcctgt taattaattt
    31141 tgacatgctt tgagatgttt aacactattc ctacagctcc cataaagatg gtgagtgaag
    31201 ttcctccata acttataaat ggtagtgcta cacctgtagc tggcattgat gatgtcgcaa
    31261 ctgctatatt taaagctgct tgtattccaa tttgtgcacc tatacctata acaaccatac
    31321 aagcaaatac attagaagtc ttcaaagcta ttcttacaca tctgtagact aaaactacaa
    31381 atagcataat aactattata caaccaatca atcctaactc ttctcctatt atagcaaata
    31441 taaagtcatt ttgtggctct ggtatataaa aatacttttg tttactcttt cctaagccta
    31501 gaccaaacaa tccacctgaa cctagtgcat atagaccttg tattacctga taccccttac
    31561 caagagggtc ttgaaaaggg tctaaaaatg atgtaacacg actaagtcta tagggttcag
    31621 caattactaa tgcagcaaat aaagcagccc cactccctat catagcaaat acaattttca
    31681 tatccattcc agcaacaaat atcattacaa aagtaactag aattacagta cctgctgttg
    31741 acaaatttgg ctgaagtatt atcaacgcaa aaaatatccc tggaactacg agaagtggta
    31801 caacaccttt tgtcaaagat tttattttat cataattttt ttcaattaat tttgcagtta
    31861 atattattgt agcaaacttt gcgatttctg caggttgaaa tgttaaagct cctattccca
    31921 accatctttt tgctccgttt gcttctatcc ctaaaggggt caaaactaac aataataata
    31981 ctactgctat agctccaatt gctgtagcat ttttcttcca aaaactataa tctattcttg
    32041 atgtaataat cataactata aatccaagta cagcatatat tacattcttt tttagaaaat
    32101 agtacgcatc attgtgttta aatgaagatt gtataaaact tgcactaaac accataacta
    32161 ttcctacaaa tactaaaagc atagtagtgt aaaacactac tccatcaaat tcagtcttca
    32221 cccttatgtc aatttgtttt ttcaaatttt ctttaggcat ccaactaatc tacctccatt
    32281 cttccattta agtaagttta gaggtaagag attattttaa attgtttaca ttatctttga
    32341 agtcttttcc tcttacttca aaactttcat acatatccca acttgcacaa gctggtgaaa
    32401 gaagcactat atctccactt tttgaaattt cataagaagt cttaactgct tcttccatat
    32461 cttttactat atgtatgtca ttaaatcctt tatttttagc acaattttct atatttgatg
    32521 cagtttctcc taataagacc aatgatttaa catatgactt tgcagtttca agaagttcat
    32581 caaaattact tcccttatcc atcccccctg ctataagtat tataggtcta tcataagact
    32641 gtatagcttt tatagtggaa tctggattag ttccctttga atcatttaca tatattacat
    32701 tgtctaaatt tcttacaaac tcttgtctgt gttccactcc tttaaatgtc ataagtacat
    32761 cctttataac ttctaaatca attttgcaga catatgctat tgctatagct gccatacaat
    32821 tttctaagtt atgacctcca ggtaaactta catcatcttt atttagaaat ttgattttat
    32881 catctatatc tattattatg tcattatttt ctaaatatac tcctccattt acttttttag
    32941 ttcttgaaaa gtaaattact ttggcattgc atttatcagc taaagatttt actatctcat
    33001 catcatagtt taatacacag aaatcctcaa cagtttggtt cataaatata tttgcctttg
    33061 cttctatgta tttttccata gtatggtgtc tatttaaatg gtcctctgta atatttaata
    33121 ttgcgcttac tttaggtcta aattcatcta tactctcaag ctgaaaactg cttagttcag
    33181 ttactaacac agactcttca cttgaagttt ctatagtatc tattacagga tttcctatat
    33241 taccaactac atatgtatct cgttttgctc ttgaaaaaat ttctcctacc aaactagttg
    33301 tagttgtctt tccatttgta cctgtaatcc ctataaaaat tggattatcc gatagtttaa
    33361 aagctaattc tacctcacca attatatact tgcctgaatt ttttaatttc aatatgaatg
    33421 gtaggtctag tggtacacct ggagacacta caaccatatc tatatcatct acatcttctg
    33481 ggtgatgtcc taatatgtat tttatgtcat ttatagattt caattcatca agtatatttc
    33541 tcaatttatt ttcatctttt atatcattta cgattatact tgcacccaat ttatctaaat
    33601 gtttaattgt agatatacca gtttttgcaa gtcctactaa taaaactttt tttcctttta
    33661 agtccatttt tcattccccc tagccctaaa atactgctat tatacttatc caagctaaaa
    33721 caactgttat aatccaaaaa ataaatacaa ctcttgtttc tggccaacca cactgttcaa
    33781 agtggtggtg tattggagcc atcttaaaga atctctttct tgttgcctta aaatatccaa
    33841 cttgaatcaa aactgataga gcctccgcaa aataaattcc tccaattata ggtattataa
    33901 gcactgagtt agttagaact gaaaatgcta caactgcacc tcctaatgcc attgaacctg
    33961 tgtcgcccat aaacactcta gccggatatg aattaaatcc taagaaacct aaacatgctc
    34021 caacagttgc tgctgctaaa actgcgactt ctgtatttcc agcaatagaa cttgcaaata
    34081 gcataaagaa tacagaaact attaatgtta ctcctgatgc taatccatct aatccatcag
    34141 tcaagtttac agcatttact attgctacga ttataaacat cattattggt atatataata
    34201 ttccaacatt tatcacaaaa tctgtaaatg gtatcataag ttgtgaagca cttgatgatg
    34261 atgtatactg ataaaaagca acataaaaag ataatgcaac ttgaaggatt attttttgat
    34321 atgcttttag tccaagtgat ctttttagct ttatttttat aaaatcatct aaaaagccaa
    34381 taaaaccaaa acctgcaata catataagac ctactgccat atcatgacta acttttactc
    34441 ttgtaagtcc agttattaga attgcaacaa tcattataat tccacccata gtaggtgttc
    34501 cgtttttagc tagatgtgtt tggggtccat catctctaac tgtctgacca aatttaaatt
    34561 ttctaagcat tggtataaat attggtccta ttataattac tattaaaaac gcaatcaatg
    34621 cggtgtacgt aagctctgtt attcctaaca tcataaaact cctctccctt gtcaattaac
    34681 tcattagttt aagcttattt atttagatat tcgactattt tttctaatct catgcctctt
    34741 gagcctttta ctaaaatagt gtctcctgtt tttactaatt catttaactt gttaaacaca
    34801 tcttctctat tctcaaagtg atatatattg gctgaattga atcctaattg ttttgcttct
    34861 tctccaataa acactgaatt ttcacctata gttattataa tatcagtatt attcatagat
    34921 gattttccaa ctaatctatg accatactct gaaatttcac ccatttctag tatatctcca
    34981 agtatcgcta cccttctacc tttatatctt ccaagtatct ttaaagcagc atccatagaa
    35041 tctggactag cattatatga atcattaatt attgttaatt tttcattttt tattatgtct
    35101 agtctcattt ttgtcccttt aaaatttttt aaaccttttt ttatataatc taaagaaata
    35161 tttagacaca aaccaactaa tatagcagac attgcattat atatattatg ttctcctatt
    35221 gttggtataa atatttcctc ttttttccca tctattacac atataaatgt taaactatcc
    35281 tcttccatag tataactttc acaataaaca tcattgtttt tattaaagcc aaatgttttt
    35341 aacttatata catgttcttt ttcttttaat gtagataaaa acttatcatc tccatttaca
    35401 attaaagtat ttgtttcatc aaagtttgaa gtaatctcca tctttgcttt caaaataccc
    35461 tcttgtgaac ctagtttttc tacatgtgat aatcctatat tcgatatgac tgcaatttta
    35521 ggatttacta tatccactaa atattttatt tcattgaatc cagacatacc catctctatt
    35581 acagcacact catgttcttt atttagatta aataaagtca atggaactcc aaaatgatta
    35641 tttaagtttt tttcattttt tagtatattt aactttgcag atatggcaga gtaaatcata
    35701 tctctagtag tagtttttcc cacactacct gtaacaccta taaatggaat atcaaatttt
    35761 tctttatagt attttgaaat atctcctaga gccaaagatg tatcttttac ctctattaaa
    35821 tttatatccg aactttctaa ttttattcca ttactttcat tttttataaa agttttacat
    35881 ccactatcat atgcagactt cataaatgta tgcccatcta aattttctcc aactatggct
    35941 acaaatgcat tttgggcatt tgcctccctg ctatctataa ctatatcact tacacagtca
    36001 ttataatctc catatattaa cttcccatta gttgcaagta ctaactcttt tattgttaag
    36061 cactccatta ttaaactcct cctttaaaac cttagctata ttctcttagc aacaaaaggc
    36121 aatgtatacc gttatatata cattgcctac gataactctt aactttataa ttatatacta
    36181 tatttctgtt tttttgaaga tgtttatggg tatatgttaa ttatctgtta ctttttagtt
    36241 ttcaaaatat agggttatac ttgagtcttc attaactttt actccaggtt ttgggaatat
    36301 atcctttacc tttgtatctt ctggaagttc tatatctgca tctaagtctg gttttaactt
    36361 cgcatcctcc aatgccttta cagcatctcc tatttttaga tttcttacat ctggcacttt
    36421 aacttgcttc ttttcatact cagctttttc ttcttcttta tatactggtt taactcccaa
    36481 atattttaaa gagtcattca ttatttcttt cactattggt cctgctgtag tactaccaaa
    36541 tgcacttact cctgttggtt cgtcaacaat agcaagaact actatttgcg ggtcatcaca
    36601 aggtgctatt ccaacaaatg aacatatata ctttcctgga gcatacttac catctataac
    36661 tttttgagct gtacctgttt taccaccaag tctataacct ggtatatacg ctatctttcc
    36721 tccaccttca gttacaactg attctgcaat ttctaacatt tttttagatg tttctttaga
    36781 tataacactt cttacttttt ttggttttac tgtttcagtt atatttcctt tattgtcagt
    36841 ataagatttt accactcttg gttgcattaa atctccacca ttagctattg atgatatagc
    36901 tgttataagt tgtattggag ttactgatat agattgacca aatgatattg tagccaattc
    36961 aactggtcct acattttttt cgttatacag tattccctta gcttctcctg gtaaatctat
    37021 acctgtctta tccataagac caaatgattc tatataatca tacatttttc caacacctaa
    37081 tctgcttcct aactcaacaa atactgggtt gcaagagttt tgtactgctt gtttaaattc
    37141 ttgtgttcca tgaggtctgt aatgtctcca acatttaatc tttcttcctc caacagttac
    37201 actacctgta catgtgaatt tttctccatc tttaattact ccttcttcaa gagcactgga
    37261 agatgtaata agcttaaatg tagaacctgg ttcatatgta tcacttactg ctggatttct
    37321 ccacatttga taatagcctt ttattttatc tttatcatta tatttttcaa gttcttcttg
    37381 ataatatgga tatattggcg tccttgagtc attagggtca taatctggtt ttgatgctaa
    37441 agcaagtata tcacctgttt tagggttcat agctattgca gtaacctttt ttgcattatt
    37501 taactcataa gctttctgta ctgctttttc agtatagtgt tgaattactt catctataga
    37561 caacacaaga ccattacctt gtacaggctc ataatatttt tccattcctt gtggtatttc
    37621 tcttcctgaa gcatcagtac ttactataag ttttccaggc tttcctttta actttttatc
    37681 atattgcatt tctacaccag atatacctgt tgcatctgaa gaagtatgtc ctaacacata
    37741 tggtgcaaaa ttcccatatg ggtaatatct ttgattatct tctgccaccc atattccact
    37801 taatttagca tctcttattt gacttgcctt atcatcatct atccatcttt ttacttttac
    37861 aagagccata ttttttttgc ttattaaagc gtaaatatct ttataatctt catccaaaat
    37921 ctcagctacc ttttcggctg cttctttttt atcttctact tccacaggtt tacaccaaac
    37981 agtatactta gttacactta ctgctaactc tttcatattt ctatcatata tagttcctct
    38041 tttgggttct attggtatat ctcttgtttg ttgttctagt gcttttgtac tcaaccaatt
    38101 tcccttcata agttgtagat acccagtcct aataacaagg caaaaaaaca atgcacatgc
    38161 tagaataaga acgagtacca gccttttctt acttatcctc tttacttttc tcaaatctat
    38221 tccccctagt tatccacatc tatatacttt atttgtccat cttttggata gtccatattg
    38281 agcaattcct tagcctttag ctcaatttct tttattgatg ttttttcagt ttcttttgcc
    38341 tttagttcct ctaatactat ctcttttttt cttaaatctt tttgtaaata atagatatcg
    38401 tacttcattt ccgagatttt tgaataacca catagattca atattattac tgaaaaagtt
    38461 gcaatcagta aaatgcattt agctcctatt tttttcttat ttctcttttt ataattattc
    38521 ttttttttat ttttatattc atttagatta tgtattttat aatttttttt caaattctca
    38581 ccccgatagt atatagttta aattttcctc tatactatat ttgttattaa tcatatttct
    38641 tcctctaaaa tttcatcgct aaattaatac actattacac aatctaattg tatttttttc
    38701 tatacacaac aaaaacctat aaattcactt atacttttat atgaatctat aggttagata
    38761 ttaatttcta tttattttta tcacttctag tatttttaga taatttcttc cataaatata
    38821 atatgtaact aactatctac tctctgattc ctaagttctc cattatatta cttaatacca
    38881 tctatatcta tatttttata tctaaatttt cattatatat ctcaataaca aagaacattt
    38941 cttatagtgt taattgtttt ttaattgctt taccagtgca acaaaatata taatactttt
    39001 taattaatta taataatctt atatataaga ttattcttta gccttataac tacttatatt
    39061 ttttctgcta ctcttagctt agcacttctt gaacgtggat ttacttctat ttcttcctca
    39121 ctaggtaata taggttttct agttattatt ttcacctcag attctttatc acattggcat
    39181 ataggtaaat gctgtggaca tatacaatct gatgctaaat gcttaaatgc atttttcact
    39241 attctatctt ctaatgaatg gaatgttatt atacatattc ttccaccttc attcattata
    39301 gaagaagcat catttatcat ctttgtaatt actccaagtt cattatttac ttctattcgt
    39361 attgcctgaa aagttctttt agctgggtga ggtccatcta ttctggcttt cttagggata
    39421 gcttttttaa ttacatctac taattctcct gtagtttcta ttggtttgtt tgctctttct
    39481 tcaactataa atttagctat acgcttagcc cagttatctt ctccataatc ttttattatc
    39541 ttagttaatt catcttctgt ataagtattt acaacatcat atgcagaaaa ctcacaccta
    39601 acatccattc tcatatcaag tggtgcatct tgcatataag aaaatcctct atcagcttca
    39661 tcaagttgat gagatgaaac tcctaaatca gctaatacac catcaatttt gtaaactcct
    39721 attttttcta attcctcttt tatattttca aaattgctgt gaacaaactt cactctactt
    39781 tcatattcac tcaatctctc ttttgctgtg cttatagcat ttttatcttg gtcaaaccct
    39841 ataaataatc ccttatctga cagttttttt actatttctt tagaatgtcc tgctcctccc
    39901 atagtacagt caacatacac gccatctggc tttatattta aattctctat acactcattt
    39961 aaaagaactg atacatgatg aaattccata gttacctccc ttaaattcac tttttaaact
    40021 attcttcact aatattattt ttatttttct ttgataaata aacatgtgca attatgccac
    40081 caataactcc aattagactt ataacttgtg ccattctaag tggtcctaac attaaactat
    40141 ctgttcttaa tccctctata aaaaatcttc ctattgaata taaagttata taagtaacta
    40201 taacttgacc ttcatatttt ttattttttc taaatagtaa taaaactata aatattccaa
    40261 aatcccatat agactcatat aaaaaagttg gatgtacctt tactccatca accattattc
    40321 cccaaggtaa attggtagca cctccatgtg cttctccatt aataaaattt ccccatcttc
    40381 caatagcttg tgctaaaggc attccaagta taactgtatc agccattttt aagaagttaa
    40441 tatttttaat cttagtgtat atataaccag ctaatattcc accaattaac cctccatgta
    40501 tagcaagtcc accacctcta aaattaaaaa tttgagacat attttgagca tagtaactcc
    40561 aattaaatac aacataatag attcttgcac agattagtcc aactggaatt gctattatag
    40621 ctatatttaa tacatcatct tctttaattc ccactctttt tgcttctttt attgctatta
    40681 aagttcctaa aatcattcca catgccataa gtattccata ccacatgatg tctatgccaa
    40741 acagtgtaaa tgctactcta tccataacgt cacctctttg tattaattga aaatatttcc
    40801 aaagcaatat gctataatta caattaattg taattatact aaattatgat aacacattaa
    40861 tgttaattct acaaaaaata ttaaaattta ttctacaact ccctacacta tattatatct
    40921 aattactata aaatttttta aactaaatct ctcatgacta aagttacaag tgtacataga
    40981 tattttttaa tcaaaaagat tgatgcatat atacatcaac ctcttctaat tcttatctat
    41041 agctcttaaa tttttacctc aatttatata aatatatttt ataaatttgt tttaccaaaa
    41101 taaacattta aaaacataaa ctaatactta attagtt
//
LOCUS       DAYAOX010000010        39814 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.010, whole genome shotgun sequence.
ACCESSION   DAYAOX010000010 DAYAOX010000000
VERSION     DAYAOX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.010"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            66..1124
                     /locus_tag="ACGI57_000421"
     CDS             66..1124
                     /locus_tag="ACGI57_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFG9715509.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPEKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            1375..3519
                     /locus_tag="ACGI57_000422"
     CDS             1375..3519
                     /locus_tag="ACGI57_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1789..1791,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFG9715510.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            3568..4401
                     /gene="fdhD"
                     /locus_tag="ACGI57_000423"
     CDS             3568..4401
                     /gene="fdhD"
                     /locus_tag="ACGI57_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFG9715511.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            4412..4585
                     /locus_tag="ACGI57_000424"
     CDS             4412..4585
                     /locus_tag="ACGI57_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715512.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            4643..5194
                     /locus_tag="ACGI57_000425"
     CDS             4643..5194
                     /locus_tag="ACGI57_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9715513.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            5219..6595
                     /locus_tag="ACGI57_000426"
     CDS             5219..6595
                     /locus_tag="ACGI57_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HFG9715514.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            6595..7110
                     /locus_tag="ACGI57_000427"
     CDS             6595..7110
                     /locus_tag="ACGI57_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9715515.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(7188..8471)
                     /locus_tag="ACGI57_000428"
     CDS             complement(7188..8471)
                     /locus_tag="ACGI57_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HFG9715516.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            8659..9081
                     /locus_tag="ACGI57_000429"
     CDS             8659..9081
                     /locus_tag="ACGI57_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HFG9715517.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            9183..10121
                     /locus_tag="ACGI57_000430"
     CDS             9183..10121
                     /locus_tag="ACGI57_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFG9715518.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            10307..12331
                     /gene="ligA"
                     /locus_tag="ACGI57_000431"
     CDS             10307..12331
                     /gene="ligA"
                     /locus_tag="ACGI57_000431"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFG9715519.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            12491..13840
                     /gene="rph"
                     /locus_tag="ACGI57_000432"
     CDS             12491..13840
                     /gene="rph"
                     /locus_tag="ACGI57_000432"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFG9715520.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRTIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            13840..14310
                     /locus_tag="ACGI57_000433"
     CDS             13840..14310
                     /locus_tag="ACGI57_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9715521.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            14504..15790
                     /gene="tig"
                     /locus_tag="ACGI57_000434"
     CDS             14504..15790
                     /gene="tig"
                     /locus_tag="ACGI57_000434"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFG9715522.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            15940..16524
                     /gene="clpP"
                     /locus_tag="ACGI57_000435"
     CDS             15940..16524
                     /gene="clpP"
                     /locus_tag="ACGI57_000435"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFG9715523.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            16543..17793
                     /gene="clpX"
                     /locus_tag="ACGI57_000436"
     CDS             16543..17793
                     /gene="clpX"
                     /locus_tag="ACGI57_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFG9715524.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(17864..18391)
                     /locus_tag="ACGI57_000437"
     CDS             complement(17864..18391)
                     /locus_tag="ACGI57_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFG9715525.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(18379..18891)
                     /locus_tag="ACGI57_000438"
     CDS             complement(18379..18891)
                     /locus_tag="ACGI57_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFG9715526.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            19071..21434
                     /gene="lon"
                     /locus_tag="ACGI57_000439"
     CDS             19071..21434
                     /gene="lon"
                     /locus_tag="ACGI57_000439"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFG9715527.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            21424..22035
                     /gene="yihA"
                     /locus_tag="ACGI57_000440"
     CDS             21424..22035
                     /gene="yihA"
                     /locus_tag="ACGI57_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFG9715528.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(22300..23670)
                     /locus_tag="ACGI57_000441"
     CDS             complement(22300..23670)
                     /locus_tag="ACGI57_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9715529.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(23845..23960)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            24248..25327
                     /locus_tag="ACGI57_000442"
     CDS             24248..25327
                     /locus_tag="ACGI57_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9715530.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            25718..26398
                     /locus_tag="ACGI57_000443"
     CDS             25718..26398
                     /locus_tag="ACGI57_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFG9715531.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            26391..27770
                     /locus_tag="ACGI57_000444"
     CDS             26391..27770
                     /locus_tag="ACGI57_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HFG9715532.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            27941..29026
                     /locus_tag="ACGI57_000445"
     CDS             27941..29026
                     /locus_tag="ACGI57_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFG9715533.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            29080..29439
                     /locus_tag="ACGI57_000446"
     CDS             29080..29439
                     /locus_tag="ACGI57_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFG9715534.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            29492..30364
                     /locus_tag="ACGI57_000447"
     CDS             29492..30364
                     /locus_tag="ACGI57_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HFG9715535.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKNIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRGFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            30482..30808
                     /locus_tag="ACGI57_000448"
     CDS             30482..30808
                     /locus_tag="ACGI57_000448"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715536.1"
                     /translation="MYVKSINELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEI
                     KKIYETIGINSITKISIDKNKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYY
                     RFRIKS"
     gene            30823..31407
                     /locus_tag="ACGI57_000449"
     CDS             30823..31407
                     /locus_tag="ACGI57_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715537.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            31660..32697
                     /locus_tag="ACGI57_000450"
     CDS             31660..32697
                     /locus_tag="ACGI57_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HFG9715538.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLNGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            32778..33143
                     /locus_tag="ACGI57_000451"
     CDS             32778..33143
                     /locus_tag="ACGI57_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715539.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            33148..33465
                     /locus_tag="ACGI57_000452"
     CDS             33148..33465
                     /locus_tag="ACGI57_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715540.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            33513..35117
                     /locus_tag="ACGI57_000453"
     CDS             33513..35117
                     /locus_tag="ACGI57_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFG9715541.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            35241..36869
                     /locus_tag="ACGI57_000454"
     CDS             35241..36869
                     /locus_tag="ACGI57_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFG9715542.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            37134..38483
                     /locus_tag="ACGI57_000455"
     CDS             37134..38483
                     /locus_tag="ACGI57_000455"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFG9715543.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            38639..39718
                     /locus_tag="ACGI57_000456"
     CDS             38639..39718
                     /locus_tag="ACGI57_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFG9715544.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
BASE COUNT        16367 a         4247 c         7166 g        12034 t
ORIGIN      
        1 attcatagtg tatactaata atatagtatt tagtataaat cttgagcaaa ggatggttat
       61 ttctaatgga tagactaaag aatattagaa agagtttact acagtataca tttttatttt
      121 tgttaatagg tataacaatt tacctagtgt ttaaaacatt ggatattaaa atgctatcaa
      181 atgttatggt tatggtagat aagaagtttt tattcatagg tgctttggca ataatgtgtc
      241 acataatgct tgaaggagta gtaatgaaga taattataga aagcactcac aaagttaata
      301 ttagatttat tggttttaag ctagccatta tgggatttta ttataattta ataactcctt
      361 ttgcttcagg aagccaacca gtacagatat atgtacttaa aaaatgtaaa atgccactta
      421 gtaaggcaag tgcagttgtt accaataagt ctattatctt ccaaatagta gttacttttt
      481 attgtacaat acttgtttta atgaattttg ctatgttaga aaaacaaatg aaaatgatta
      541 tgcctctagt gtttctaggg atagccatta attcatttgt cttaattatg ataatattag
      601 ttattttaaa tcctgaaaaa ataaaatatt ttatgagaat tactataaag catttatcaa
      661 aaattaagtt tttaaaattt ttagataata agattgaatc catagaaagt tttatagatg
      721 attatagtaa agcaataaat ttttttgcaa agaataaaaa ggtattaatt tcaactataa
      781 tagtaacttt tattcaatta agtgcttact ttagtgtttc attttggata tataaagcat
      841 ttaatttaca aggttatagc tatatttata tactgacatt acaagcattt ttatatatgg
      901 caatatctcc aataccaaca cctgggaatg taggagctaa tgaattagcc tttttcacta
      961 tattcaaatc agtattccct caacctttga tgggatatgc agtattttta tatggtggat
     1021 ttatgtacta tttgattctg attggaagtg gaatatttac tgtaattact cactatagaa
     1081 tgaaaaacag aattggcaga aatattgttt taagtgaaaa ctaaataagt ttaaagaata
     1141 tatattgagt atattaaaaa atatgtcata agaactagaa tattttaatt aaatggttga
     1201 aatataaaga ttattatgac atattttttt tgaaatttaa gaggaattgt gagaaaattg
     1261 ttgaatttaa tagatagtat tgcaaaatat ttatgatttt gcatattata aaaacactca
     1321 ttatctttaa gataaatttt acttatcagg ggataaatct aaggggggaa ttgtatggag
     1381 aaaaaaattt taacagtttg tccatactgt ggagctggat gccaattata tctagtagta
     1441 aaagataata agatattaag agctgaaccg gctaatggta gaacaaatca agaaagtttg
     1501 tgtttaaaag gtcgttatgg atgggatttt ctaaatgacc ctaaaattct aacacctagg
     1561 ctaaaaaagc caatgataag aaaacaaggt gtactagaag aggttgaatg ggatgaggct
     1621 ataaactata cagcttcaag actaaatgaa attaaagaaa aatatggacc tgattctatt
     1681 atgggaacag gttctgcaag aggacctggt aatgaagcaa actatataat gcaaaaattt
     1741 atgagagcta caataggaac taataatgtt gaccattgtg ctcgtgtctg acatgcacca
     1801 tctgtagccg gtctggctta ctcattaggt agtggtgcaa tgtctaattc tataccagag
     1861 atagaaaatg cagatgtttt atttatattt ggatataatg gagcagattc acatccaata
     1921 gtggcaaata gaatagtaaa agctaaaaaa aatggtgcta aattaattgt tactgaccct
     1981 agagtaactg aatctgcaag aattgctgat atacatctac ctataaaagg tggaacaaac
     2041 atggttttag taaatgcttt tggaaatgta ttaatagaag aaggtcttta caataaagaa
     2101 tttgttcaaa atcatacaca aggatttgat gagtataaag aaattgttaa accatataca
     2161 gctaaatatg ctgaaaagat tacaggaatc ccagaagagc ttataagaaa agctatgaga
     2221 gaatatgcaa aaggtaaaaa agctatgata ctttatggaa tgggtgtctg tcaatttggt
     2281 caagctgttg atgtagttaa gggacttgca tcaattgctc tacttacagg taattttggt
     2341 agagaaagcg ttggaatagg acctgtacgt ggacaaaata atgttcaagg agcttgtgat
     2401 atgggagctc ttcctaatgt atatcctggc tatcaaaatg taactgatga taagataaga
     2461 gaaaagtttg aaaaagcttg gggggtaaaa ttatctccta ataatggtta tagtttaact
     2521 caagttccaa atttagtact aaaagagaaa aagcttaaag cttattatat atttggagaa
     2581 gacccagtgc aaagtgaccc agatgcatca gaagttagag aagcattaga tgaattggaa
     2641 tttgtcattg ttcaagatat atttatgaat aagacagcac ttcatgctga tgttatactt
     2701 ccagctactt cttggggaga acatgaggga gtatatacat gtgcagatag aggattccaa
     2761 ttgatgagaa aagctataga gccacaagga gatgtaaaac cagactggca aattataagc
     2821 gaaatttcta cagcaatggg atatcctatg aattataaaa atactaagga aatatgggac
     2881 gaacttagac aattatgtcc aagtttctta ggtgctactt atgaaaagat agaaactcaa
     2941 ggatgcgttc aatggccttg taagagtgaa agtatggaag ataaaggaac tatgtatctg
     3001 tatgaaggtc aaaagttttc tactcctaat ggtaaaggta atttgttcgc tgctgaatgg
     3061 agacccccta tggaagttga agatgatgaa tacccattta gtttatgtac tgtaagagaa
     3121 gttggacact attcagtaag aacaatgact ggtaattgta gaacacttag ttcattagaa
     3181 gatgaaccag gacgtgttca aataaattct aatgatgctg aaaaattagg aattgaagat
     3241 gatgaattag ttagaattag ttcaagaaga ggaagtgtaa taacaagagc aacagttact
     3301 gatagagtta aggaaggtgc aacatatatg acttatcagt ggtgggttgg agcttgcaat
     3361 gaacttacaa tagcaaattt agaccctata tctaaaacac cagagtataa atattgtgct
     3421 gttaaacttg aaaaacttga agaccaagaa ttagcagaaa aatgtgtaag agaagagtat
     3481 caaagtttga aagacaaaat gacagctaca aatatctaat gatttattag atttaagatt
     3541 tataaaaggc agaaaggcta atattttatg aacaatatat actcaaaaac taaattacaa
     3601 gaaaatagta aggataaagt ttttgaaaat agttatagtc tagtggacta caaatattta
     3661 aattctttta atgtagaagt aagcaaaata aatgaacatg aagtatcaat agataatgaa
     3721 gaaataattg cagaatatcc acttagtttt attttaaatg ataaatatgt aaatacattt
     3781 ttatgtaccc catataattt agaggaactt atagtaggat tcttgaaaac aaaaggatac
     3841 atagaaaaca aaaaaagctt attaaaattt gagataaatg agagtgaacg ttttgcaaaa
     3901 gtaactattc aagagaaaca aaaaaatcaa gatgagcaaa ttatattttt aaattcacta
     3961 gactatataa catgtgctcc tgtaaagagc aatataaaaa taaattatga tacgatttac
     4021 agtataatgg ataaaaattt aaattcatca gaattattta aaaatactgg tggggtacat
     4081 agcgtatctg tatttaattt tgataaaaaa atagtcactt gtgaagatgt agcaaggcat
     4141 aatgcaatgg ataaggctat tggattttgt gttttaaatg acataacatt aaaagataaa
     4201 attgtggctg taagtggaag aatatcattg gaaatgatat taaaagcagc tatgactcaa
     4261 attcctattg tagtatcaaa atcagcaccg actaatttat caatagagct ttctaaaaaa
     4321 ttaaatataa cgcttattgg atttgtaaga ggaagacgga tgaatattta tgcaaatcca
     4381 cagagagtta tcaaaaagtg agaatgaata tatgtgtact aaaaaagaag agcaaagata
     4441 ttcaaagtgt gaaatctgtg gagtattaat tgatgatact gataaggtgt ataataacac
     4501 atacaatttg tgcaaaaaat gtaaacgaaa tataagtact gagaaaatag tgaaatatat
     4561 aaatatatat gatgaaagta agtagacata tgatgaaaat aactagaggg ttggaaatat
     4621 acaaattgaa aggtgggata atatgaaaaa taaaataggc tcttttgtaa tagcagaccc
     4681 aaacaaatgt acaggttgta gagcttgtga agtagcttgt tttacaatgc ataatcagca
     4741 taataatgtg ggttatacag ttggaacagt tgatatacct gttataccta gactttactt
     4801 agtaaaaggt gacaatttct gtatgccaat acagtgtaga cattgtgaag atgctccttg
     4861 tttaaactca tgtccacaaa aagctatagt taaagaaaac aatattatgt ctgtaaatga
     4921 agaaaagtgt ataggatgta aaacttgtct gctagcatgt ccttttggag ctatagactt
     4981 acttccacaa tatgaagatg gtaaagaagt agaacaagta attttagatg aaaataagaa
     5041 gatagcttat aaatgtgact tatgtaaaga caatgaaaaa atagcttgta taagtgcatg
     5101 tcctcaggaa gcattaaaac ttgttactcc aatagatgac aaaaaagcta agaatagaca
     5161 agctgcactt agtttattgt taacaaataa ataaaggata caggggtagg tgacgtttat
     5221 gataataaat atagataagg atttatgtac tggatgtaga gaatgttcaa aggtctgtcc
     5281 agtaaatgct attgaaggtg aagagggaaa gccacaagag ataaatttag atagatgtgt
     5341 tatgtgcggt caatgtgttc aaacttgtaa atcttatgcc tcagttatag atgaaggatt
     5401 tgagttttta caagagaaaa aacaagaaag agagatacca gagtctataa atgaacctat
     5461 atttgcagca tacaatgtat gtgatataga taaggtgaag aaagctttaa gtgacccaaa
     5521 tgtattcact atggtccaat gtgcaccagc agttagagtt gctttaggag aagattttgg
     5581 atattattta ggatacttag gtgctgggaa aatggcagca gcacttagag ctttaaattt
     5641 tgatagagtc tacgatacta attttgcagc agatttaact ataatggaag aaggtagtga
     5701 attaattaaa agagtgacag aaggtggaac tcttccaatg tttacatctt gttgtccagc
     5761 ttgggtaaaa ttcatggaaa aaaattatcc taaattaact aatcatttat cttcttgtaa
     5821 atcacctcag cagatgggtg gtgcaatatt taaaacttat ggtgcagaga taaataatgt
     5881 agatgcatca aagatattta gtgtttctat aatgccatgt acatgtaaaa aatatgaatg
     5941 tgatagagaa gaaatggatt ctagtggata tagagatgta gatgtagtat taactactag
     6001 agaactagct tatttaataa aagacatggg aattgatttt aaaaatttaa gagaagaaaa
     6061 atttgatagt cctttaggta gttatagtgg agctggaact atctttggtg ttactggtgg
     6121 ggttatggaa gctgctataa gaacaggata tgagcttata actggagaaa gtattcctga
     6181 tgtagaagta aaacaagtaa gaggagaaaa tggttttaga aagtcaacta ttaaaatagg
     6241 agacttagac ttaagagttg gagtagtaag tggattaaaa aatgtaatac cagttcttga
     6301 agacttggaa aaaggaaaat tagatgtaga ttttattgaa gttatgactt gtcctgttgg
     6361 gtgtgtaagt ggtggaggtc aaccaaaaat tttactagaa gaatataagg aattggctta
     6421 tgaaaataga acaaaagcaa catacgtgca tgatgaacat ttagcaatta gaaaatctca
     6481 tgagaatcct gatattaaaa aactgtatga tgaatatctg gtagaaccat taggagaaaa
     6541 atctcatcat ctattacaca ctacatattg tataggaaag gaagtggcta agtgatgaac
     6601 tattttgtta tagcagaccc tgataaatgc ataggatgta gaacttgtat gataggatgt
     6661 gtagttgcac attcagatga agatattttt taccaaaatc cagatgaaat aaattttaat
     6721 ccaaaacttt ctgtcataaa gacaaaagaa gtaagtgctc caatacagtg tagacattgt
     6781 gaagatgctc cttgtgcaaa ggcatgtcca aatggtggta tagttagggt tggtaacaca
     6841 ataaaaataa atgaagaaaa ttgtatagga tgtaagactt gtatgttagc atgtcctata
     6901 ggtgcaatag acatagttac attaaaagat gtagatgaag gaaagctttg ctttagagaa
     6961 agaatgacag caaataagtg tgatttttgt atagaatcgc cagaagggcc tgcatgtgta
     7021 aatgtttgtc caactaaggc atttactata gttaaggatg aggatataga tagtaaagtt
     7081 aaaaataaga gaaaattagc tatattgtaa tataaaaaag atggagtttt atttatgatt
     7141 aaaagctcca tcttttttat tataatattt atatatctaa aaatcaatta ttgcattagt
     7201 ttatctaatc tttctttatt caattttttc attcctatag aagttataat aagtaaagta
     7261 taggcaatta taactgataa aattataata gaccaagaaa atctgtatcc aaaattatca
     7321 actaacaatc caaataatgt tgaacctaat gcaaatccca ctgcaaaaat taattgtacg
     7381 attccatata tgctaccaaa ctccttttga ccaaagaaag agccagttaa ataagcagga
     7441 ccaatcatat aagcaaatac agaaagacct ttcaaaatgg caaatacaaa tgcaaaagta
     7501 gaagatttat ctaccaatag taatgataaa gttgctataa gaaccaatac aaaagatata
     7561 ataagtggct ttgtttgacc gaatttatca aacaataatc ctccaaataa attaccaatg
     7621 agagcaaata aggcaaatgt ggagcctact gttcctatta gaataggatt tagataattt
     7681 ctaagataag ttggatattg tacagataat gatgaaacat atataccaac aaatataaat
     7741 ccaatagtca taatccaaaa atatttaatt tttttagctt cttttaatgt ataaccccat
     7801 actgaaggag aaattgtatc agatgctttc tcttttttac ctttagattt taccttttca
     7861 ttaatatctt taggcatcct gagtatgaat aatattacag gtataccaac tattaatgct
     7921 ataattccaa atattagata agaacgactg tatccttgag ttgctaataa gcgaactgca
     7981 agtggttgta gaaatatact accaatagaa ccaccagcaa aagcaaagcc caaagctttt
     8041 cctttgcttt cttcatcaaa ccaagcactt attaaaacag gtattcctat agcagatata
     8101 attgtagcgc caatttgaac aatacatgat acagtgtaaa attgccatag ttcagtacat
     8161 atagaaaaag tagcaaaagc aatactagaa attatacaac ctaataaata tagtatctta
     8221 atattgactt tagaaaataa tacacctata aaaggtgatg caattgctga ggcaatagta
     8281 ccaatagtga atattagaga aaaagaagct aaactaaagc cttctccttc tacaacataa
     8341 cttataaatt gaggctgtat acttgctaca atactaaatg gaacagcttg tattaacata
     8401 caaccaagta ctataaccca agcatttgaa aagtttttaa tgctaaattt attgtttagt
     8461 aatgtattca tggtatctct cctttaggtt atgatttgta gatattaatc taattattct
     8521 aattttatat attagttttg gatagcctaa ctatttaaat ataatttggg tattaatata
     8581 aaaattaact ataaaaattg attattttaa atgtatgggg taaattaaag tatataaata
     8641 aatgaaagga tggataaaat gtcatttgaa aaaataaata ataataacct agataaaata
     8701 cacaatagga agtgtattat gcttgtaaac tttaataaaa aagaattatc attaattaaa
     8761 aatgtaagta actttgctgg aataagagac catatcatct taagtgataa aaatggagag
     8821 aatataataa gagacatttt agatggaaat atatcaaatg aatgtgaaaa tgggattaag
     8881 agtaagtctg taatatttaa taacactcca agtacacaag taaatgcatt tttagaaggg
     8941 ctaaaaaaag tgagaataaa tagacctttt acagctatgg taacagagac atctattgat
     9001 tggacattga ataatttaat agaaaattta gttcaagaac gtaatgctat gtctttagga
     9061 aaaacaattg aacataagta aatttaagat atcttatagt aattttaact aatgtacatt
     9121 gagtatcata ttatatttta gaatagaaaa ttttaaatgt gggtagatgg aggagatttg
     9181 agatgaaagt tttatttaca ataaattatg gaaaagaaaa atttgataaa ataagagaat
     9241 taggctatga tgtcatacat tactctgaag atagtattga aaataatgag gatgtaaata
     9301 cagcagatgt actagttaca tacaatccat ttgagagatt agatatttct aagatgaaaa
     9361 atttacaata tatacagaca actagtgtag gtatagacca acttccaaaa gaagaggtat
     9421 tgaagagaaa tattaagata gctaataata gaggcggata tagtgtacct attggtgaaa
     9481 gtattgtatt gtatatttta gaaatatata aaaatagtgc aaaatttttt aaacaacaac
     9541 aaaataaaga atggaagatg gatttttctg tatcagaatt gtatggacaa agagttggat
     9601 ttttaggaac aggtactata tcttcagaag cagcaaaaag attaaaagct tttggtgtag
     9661 aaatatgggg tgtcaatact gatggaagta gtaaaaaata ctttgataag tgttttgcta
     9721 ctgaggatat gaatattgta tttgaagaat gtgatatagt agttgtagca atgccttcta
     9781 caaaaagtac agatggaata gtaaacaaag atatgtttaa acttatgaaa gatggttctg
     9841 tatttataaa tgtaggtaga ggaaatacag ttaatcagaa agatttagaa gaatgtgtag
     9901 agaaatttag aggggtagca ttagacgtat ttgaaagtga acctttaaac aaggacaata
     9961 agttgtggga gtgcgaaaat gtcatagtaa ctcctcataa ttcttgggtg tcagataaaa
    10021 acaaagagag aacatttaat atggtttata ataatttgaa acactatata atggagaata
    10081 aacctgtaga taatgttgta gacatatcaa gaggctatta aaaataaaaa attttgataa
    10141 aaataaaaac tcttattagt cgtattaaag atactaataa gagttttttt tgtaataaat
    10201 ttaaagccca aaaagtgata tactaaaaaa tatataaata ggagataatt aattataggg
    10261 aaatttcaaa ataatttaat tttaaaaata tgaagaggtg caaaaagtga gcgtaaaaga
    10321 tgaaattaat aaacttatag agacaataaa ctaccataat gaaaggtatt ataatcaaga
    10381 tagcccagaa atatcagatt atgaatacga taagttaatg aaggagttaa tttctttaga
    10441 agaagaacat ccagaactaa agagaattga ttctccaaca aatagggtag gaggtaagcc
    10501 attagataag ttcaatcaaa ttgtacataa aactcctatg ttgagtttat caaatgcttt
    10561 ttcagagcaa gatttgagag attttgataa gagagttcaa gaatatgtgg gagaaaatgt
    10621 tgaatatgtt gttgaattta aaatagatgg tctttctgta ggtctaactt ataacaatgg
    10681 agagtttgaa aaaggagcaa ctagaggaga tggagttgta ggagaagata tttctcaaaa
    10741 cttaatgaca gtaaaaagca tacctcttaa gataaatgat aaaaaagaac taatagttag
    10801 aggagaagta tatatatcaa aagataattt tagaaaagta aatgaacaac aagaagaaca
    10861 agaacagcca ttatttgcta atccaaggaa tcttgcagct ggttcattga gacaattaga
    10921 cccaaaacta acagcaaaga gaccattaga tatatttgta tttaatatgg aaaatataga
    10981 agaatacgat ttagaaagtc acagtgaatc actagagtac ttagaaaaac taggattttc
    11041 agtaagtcag agttataaag tttgtaaaag tatagatgaa gttattgagc atattgaata
    11101 ctggacagat aacaggggta atttagacta cgaaatagat ggtatggtaa taaaagtaaa
    11161 caatataaaa caaagagacg aaatgggata tacagccaaa agtcctagat gggcaattgc
    11221 atataaattc ccagcagaaa agaagaaaac taaaatagta gatataatag tagaagttgg
    11281 gagaactggt acaataacac cttcagcaat tcttgaacca gtcagacttg caggtaccac
    11341 tgtaagtaga gctacacttc acaatgaaga ttatataaga gaaaaagata taaaaataaa
    11401 tgatacagta ttagttcaaa aagcagggga cataatacct caagttttag aagttataaa
    11461 agaagaacgt actggagatg aaatagattt taaaatgcca gaaaagtgtc cagtttgttc
    11521 agagccaaca ataagattag aaggagaagc tgctgtaaaa tgtatcaata tgtcttgccc
    11581 agcccaaata agaaggggaa ttatacactt tgtgtcaagg gatgcaatga atatagatgg
    11641 attgggagaa tctataatta cactgctatt aaatgaaaaa attattcaag atgtatctga
    11701 tttatattat ataaaaaagg aagatgtagt cgacctagag agaatgggcg aaaaatctgc
    11761 tgacaattta ataaattcaa ttgaaaaatc aaaagaaaat gatttatgga gacttataaa
    11821 tggattaggt attaaattta taggagttaa agcagctaag atacttgcat ataactttaa
    11881 ggatttagat gaagtaattt ctgcaactag tgagcaacta gaagaattag aggaatttgg
    11941 aagtataatg gcaaacagcg ttgtcgagtt ttttaaggaa gacaaaaata tgaacgttat
    12001 aaacaagtta aaaaatgtag gagtaaatac aaaatcattg gataatacag gtgaaaaagt
    12061 agaaaaaata tttgaaggta tgaaaatcgt attgactggt acacttccta cattaaaaag
    12121 aaatgatgca aaagatatga tagaaaaaag aggtggaaaa gctacatcta gtgttagtaa
    12181 atcgacaaca tttgttttgg caggtgaaga agctggttct aaacttacta aagcaaatga
    12241 tttaggaatt aaagttatag atgaagagag atttttagaa ttattaaagt tatcatcgaa
    12301 agatgaagta aaagctgtat tagagagtta aaaagtcgct taatttagta gaaaactact
    12361 ataattaatg atagaatagt tcagtaagct aataagatta cgaaaaatga caataattaa
    12421 tatttgacaa aattaaaaat tataaaaata aatttataaa aaataaaaaa tatgagaaag
    12481 gatataaaaa atgaccagaa gttatgatag aagtaatgaa cagataagac ctgtaaagat
    12541 aactagagat tatacaaagt atgcagaagg ttctgttctt atagaaatgg gagagacaaa
    12601 ggttatatgc actgcatcag tggaagaaaa agttcctcct tttttaagaa atagtggtac
    12661 tggatggata aatgctgaat attctatgct accaagagca acacaacaaa gaaagataag
    12721 agatgcatct aggggaaaaa ttgatggaag aacacaagaa atacagaggt taataggaag
    12781 aacaataagg tctgttgtag acttgaataa attaggagaa agaactattt gggtagattg
    12841 tgatgtgatt caggcagatg gtggaactag aactgcatct ataacagggg catttgtagc
    12901 agttgcagaa gcaatatata aattatataa agcaaagaca ataaaagaga tgccaataac
    12961 aaattttgtg tctgcaatta gtgttggtat tgtaaatgga atacacatgc tagatttatg
    13021 ttatgaagaa gattctaaag cacaggtaga tatgaatata ataatgactg ataagtgtga
    13081 gtttgtagaa gtacagggga caggagaaga gaaacctttt tctagagcag atttaaattt
    13141 attgttagag ttaggtgaaa aagggaataa agaattaata aggcttcaaa gagaagcttt
    13201 aggtgaaata tcagatgaga tattgggtat ggattatggt aatgaagtag tgattgctac
    13261 taataatgct cataagctag aagaaatagg tgaaatctta aaagattttg aatataaagt
    13321 gtactcttta aaagatgttg atttagcagg tatagagata gtagaagatg gaaaaacatt
    13381 tgagcataat gcgttgataa aagctagaac aatagcaaaa aagactaaac ttatagcaat
    13441 atcagatgat tctggcttag aagtagatgc tttaggcaaa aaacctggtg tttattcagc
    13501 tagatatgca ggagaacatg caacagatga agaaaataga aaaaaactgt taaaagctat
    13561 gcaaaatgta ccaatgagca aaagaaatgc aagattcgtc tctgcaatag cagtagtttt
    13621 tcctgatgga aaagaatttg tagtaagagg aatttgtgaa ggaatgatag gatttgaaga
    13681 aaaaggtaaa aatggttttg gatatgatag cttatttata gtaaaaggat atgataagac
    13741 ttttggtgaa atacctagtg ttataaaaaa ttcaatcagt catagagcaa acgcactaaa
    13801 acttatgaaa caagagttta taaaaagggt agtgaagtaa tgaaaatagg catagtaagc
    13861 gacacacata tgatgattaa aaatatggaa aaaacaatac catatttgaa agaatgtgac
    13921 ctaataattc atgcaggaga taactttact gattcaagat atattcatag catgactaat
    13981 gtaggtatta ttgcagttaa aggtaattgt gactttgatg ctgttgagga agaagttgtc
    14041 tttgaagtgg caaataaaac catattctta tgccatggag ataagtatgg tgtaaaatat
    14101 ggaactaata tgttagaaaa aaaggcaact gaagttgatg ctgatatagt tatatttggc
    14161 catacccata ccccatttag agaaattaaa gatggtgtac tttacataaa tcctggaagc
    14221 acttcacttc caagaggagt atcgtataaa agttttgtca ttatggatat agaagaagat
    14281 gacataaaaa ttgaagaaat acgcatataa aggtgtattt atctaaaaaa atggcaaaaa
    14341 acgctcaaaa aattaaattt tatcaaataa gtacaacgat attaatggaa cacttgtaaa
    14401 taaaaaaact tatgtgatat aattaagtgg ttgaattata aatagaatga attataatac
    14461 cgttatcggg tattacataa atatcactag gaggattaga aggatgaaag cagaactaat
    14521 taaaaaagag ggtaataaag ttactttaaa aataacagta gataacaata aatttgaagc
    14581 agcagtaaat aaggcttata ataaaagcag aaataaatac aatatacctg gatttagaaa
    14641 aggtaaagct ccaagaattg ttatagagac tcaatacgga aaaggtatat tctacaatga
    14701 tgctatagaa atattattcc cagaagttta tccagaagct atcaaggaat tagacataga
    14761 cccaatagac aatccagatt tagatataga agaaataagt aaagacaatg ggttggttat
    14821 ggttcttaat gtagaagtta aaccagagtt tgaattagga aactacaagg gtatagaaat
    14881 agctaaggtt gagaatactg taagtgacga aaatgttgat gctaaactac aagaaatggt
    14941 tgaaaaaaat gccagattag taagtgtaga agacaaagct ttagaagatg gagatacagc
    15001 tataatagac ttcgaaggtt ttgaaaatgg tgtagctttt gatggtggta aaggtgaaaa
    15061 ttataactta gttataggtt caaacacatt tataccagga tttgaagaac aattagtagg
    15121 taaaaaagct ggtgaagaag tagaagttaa tgttactttc ccagaagaat atcactcaca
    15181 agatttagct ggaaaaccag ttgtatttaa tgtaaaaata aatgatgtaa aagtaaaaga
    15241 attatctgca cttgatgatg aatttgctaa agatactagt gaatttgatt ctttagatga
    15301 attaaaagca gatgtaagag ctaagttaga agaagaagct aaaaatagag cagatgcaga
    15361 aactagaaat tcagtagtag aaaaagttgc tgaaaataca gaaatagaaa taccagaagt
    15421 tatgatacaa catcaaatag ataatatgtt aaatgaactt aactaccaat tacaatatca
    15481 aggatttgga ttacaacaat tactagagat gactggtaga actatggaag aattaagaga
    15541 agagagaaaa gaagatgcta aaaaattagt taagtcttct ttagtattag aagcaataac
    15601 taaagctgaa ggaattgaag caacagaaga agaatttaag gctgagttag aaaaaatggc
    15661 ttctgcttac aatatggaag tagaaaaaat agaagcttct ttaagagatg ctgacaaaga
    15721 agatataaaa ggtcaaataa aaataagaaa aacaatagat ttattagttg ataatgcaac
    15781 aatagcttaa taaaataaac gctaactaat atagttgacg ttatgaaatg tttagtatat
    15841 aatgatgtgc aaatataatt tagttaaatg cgtataataa tttacagtag cttaaaaaaa
    15901 gctactgtaa attatataga ataaaggggg tatgaaacca tggcattagt acctgtagtt
    15961 gttgaacaaa caggaagagg agaaagatct tatgatattt tttctagact attaaaagac
    16021 agaataatct tcttaggaga tcaagttaac gatgccacag cagggcttat agtagcacag
    16081 ctattatttt tagaagctga agacccagac aaagatatac atttatatat aaattctcca
    16141 ggaggaagta taacttctgg aatggctata tatgatacta tgcaatacat caaaccagat
    16201 gtaagtacta tatgtatagg tatggctgct tctatgggag cattcttgtt ggcagcagga
    16261 gcaaaaggaa aaagactagc actaccaaat agtgaaataa tgatacatca accattaggt
    16321 ggagctcaag gtcaagcaac agatatagaa attcatgcaa agagaatttt aaaaataaaa
    16381 gaaactttaa atgagatatt atctgaaaga actggtcagc cattagaaaa aattaaaatg
    16441 gatactgaac gtgataactt catgagtgca ctagaagcca aagaatatgg tttaatagat
    16501 gaagtattta caaagagacc gtagatagag agaggtgtta atatgtctaa atatgaagaa
    16561 aaacgacaat taaagtgttc attctgcggt aaaaatcaag atcaagtaag aagattaatt
    16621 gcaggtccaa atgtatatat ttgtgacgag tgtgttgaac tttgtgacga aattattcag
    16681 gaagaaatag aagatactat agatgaagat actacatctt taccaaaacc aaaggaaatg
    16741 atggagattc taaatgatta tgtaataggt caagaaaaag ctaaaaaagc tttatcagtt
    16801 gcggtttata atcattataa gagaatatac agtaaaaaat ctagtagtaa ggatatagaa
    16861 attcaaaaga gtaatatact tttattagga cctacaggtt ctggaaagac attacttgct
    16921 caaactctag caagaactct aaatgtacct tttgcaatgg cagatgctac atcattgact
    16981 gaagctggat atgttggaga agacgttgaa aatatacttt taaaacttat tcaagctgct
    17041 gattttgata tagaaaaagc tgaaagaggt ataatatata tagatgagat agataagatt
    17101 gcaagaaaat ctgaaaaccc atctataact agagatgtta gtggtgaagg tgttcaacaa
    17161 gcacttctaa aaatattaga aggtactgtt gcaaatgttc caccacaagg aggaagaaaa
    17221 catcctcatc aagaattttt aaagatagat actactaatg tattatttat attaggtgga
    17281 gcttttgatg gattagagaa gataattcaa aaacgtggtg gagataaaac actaggtttt
    17341 ggagctaaaa tagaaagtaa aaaagaatta gatttaggga agctgtatga aaaagtactt
    17401 ccagaggatt tattaaaata tggtataata ccagagttta taggaagaat accagtttta
    17461 gctactttag agttgttgga tgaagatgca ttaatgcaga tattacagga acctaaaaat
    17521 gctctagtaa aacagtataa aaagttattg gagttagatg atgttgagct agaatttgaa
    17581 gaaggtgctc ttcgtgcaat cgctaaaaag gccatagaaa gaaatacagg agctagagga
    17641 cttagaagta tagttgaaag cgtaatgatg gaaactatgt ttgaagtacc atctagagat
    17701 aatataaaga aggttatagt aacagagaaa tctgtgaacg aagactcagt aaatcctatc
    17761 atagtactta aggatcaaga ggaaagtgca taagatgatg aaaaggagct ttaaaagctc
    17821 ctttttttat gttaatatgc taaatataaa ataggtatca ttattataaa aatgtgagta
    17881 atatacctag aataccaaag acgaatacaa ttgcaatgga accaattttt cctttatata
    17941 aaagataaaa tgatgctata aatgcaatta ttccatataa atcccaatta acttctttgg
    18001 cagatgaaaa agctgctgat aatatgaatc ctagagtaat tggtctaaga actgtcaata
    18061 agttttctac ctttttatta tttttaaatt tagaaaataa ctttgatata atagttagac
    18121 ctattaatgc aaaaattgta acaccaatag ttgcagctag agaaccaaag aagccagcag
    18181 tcttaaatcc tacaaatgta gcactattta ttgctacagg acctggtgta gattgtgata
    18241 tagcaagtaa atccaaaaac tcttgattag ttataaagtg atatttagat ataaattctt
    18301 gctggataaa tggtagcatg gcataaccgc caccaaaact aaatgcccct attttaagga
    18361 atattaagaa gagtagtatt atttctgaca tagactatat acacctcctc caattattaa
    18421 ataaataggg cttatattga atacaccaac agataaaact gctataatta atagtagtat
    18481 gttagtttta gactttggta gtttcttgaa catgctcgta aaagaataca atataagagc
    18541 tattacaaca ggcgcaactc ctttaaaaaa tccatctaaa attttagatg atgaattttt
    18601 aaagtataga tacgataaga ctaagactat acaaaatgat ggtaaagcag agcctaaggt
    18661 acatataaga actcctggta taccataaag tttatatcct aataatacag atatattaac
    18721 tgccagtact ccaggataac tttgagctat ggataaataa tcaataaatt cttcctgctc
    18781 taaaagtttt tgtttgttta caagttcatc ttgtataaga ggaaccatag cgtatccacc
    18841 accaaatgta aacgcaccaa tttttaaaaa ggttaaaaac aatttcaaca taatagaaac
    18901 ctccaaaaga taaataacat ctaagtatac taatattaat attatatcat aataaaaaga
    18961 taatatagta tgatagtatt ctaagaattt atcattaatg ttgaaaatta acttgttcaa
    19021 atatataata ttaaaagcaa agcaattaga ctagaaaagg agtaatgttg atggaacaaa
    19081 attatactaa aatagatcat gaattaccat taattcctct aagaggatta gctatatttc
    19141 catatatgat attaaatttt gatataggta gagagatatc tcttaaagct ttagaccaag
    19201 ctatgatgga tgaagaactt atatttttaa cttctcagaa agaagcagag gtagatgagc
    19261 caggagaaga ggatttttat catgtgggaa caatctgtaa agttaagcaa atgataaaat
    19321 taccaggaga tacagttcgt gttttggttg aaggtgtatc aaggggtaga gtaaagaaaa
    19381 tagaacaaga agatgggtat ttcagagcag taatagaaga aatagtattt gatagtgata
    19441 atttggatag tgaaacagaa gttgaaattg aagcatttgt aagaaatgta tttgatgcct
    19501 ttgaagagta tataaatatt ggaaataggg tatcaccaga aatattaata tctttagctg
    19561 atatagaaga tgtagataga tttatagata cgatagctgc aaacatatat ctaaaatcaa
    19621 gccaaaaaca agaaatatta gaagaatttg atattagaaa aagacttgaa cttatttatt
    19681 ctatattatt agaggaaata gatatattaa aaatagaaaa gaaaataact ttaagagtaa
    19741 aaaaacagat gaataaggtt caaaaagaat attatcttag agaacaatta aaagctatac
    19801 agaaagaatt aggtgaagaa gaagatataa actctgaagc agatgagtat agagaaaagc
    19861 ttaaaaaaat aaaagcacct aagacaacta aagagaaaat agaaaaagag atagataaat
    19921 tttcaaaaat atcttctatg tctccagatg tatctgtaag tagaaactat ttagatacta
    19981 tattctcatt gccatggaat aaagagacaa aagataaatt agatataact aaggcaaaag
    20041 atatattaga tgaagaccat tatggattag aaaaagttaa ggagagaata ctagagtatc
    20101 tagctataag aactcttgct aagtcactta aagggcctat aatatgtcta gttggtcctc
    20161 caggaacagg aaagacatct atagtaaaat caatcgccag agcattaaat agaaaatttg
    20221 taagaatatc tttgggtgga gttagagatg aagcagaaat aagaggtcat agaagaactt
    20281 atgtaggttc aattccagga agaataatta atggggtaaa agaagcccaa acaaaaaatc
    20341 ctgtattctt atttgatgag atagataaga tggcagcaga ttataaaggt gacccagctt
    20401 cagcaatgct agaagtatta gacccagagc aaaataagga ttttgtggac cattacttag
    20461 aaataccttt tgatttgtca aaaatactat ttgttacaac agcaaatagt ctaagtaata
    20521 taccaagacc attattagat agaatggaag taatagaagt atctggatat atagaagaag
    20581 aaaaattaaa tatagcaaaa aaatacctac ttccaaaaca aattaaagaa catgctttaa
    20641 aagaaaactt tataaaaata gatgatgaaa ctttaagaag tataatcaac cattatacaa
    20701 gagaagctgg tgtaagaaca ctagaaagaa ctattggtaa gatttgtaga aaggttgcca
    20761 aaaaatacgt tgaagaccca actcttgaag aggttgttat aaataagtct gacctagaga
    20821 catatcttgg aaaagacatg tttaagtatc aacttgcaga agttaaccct caaataggat
    20881 tagtaaatgg tcttgcatgg acagaagttg gtggagttac acttgaagta gaagtaaacg
    20941 tacttaaagg taaaggagaa attgtattaa ctggaaaatt aggagatgtt atgaaggaat
    21001 ctgcgaagac aggtatctct tatataaggt caatagtgga caagtttgat atagaccctg
    21061 aattttataa gacgaatgat attcatatac acattccaga aggtgctgtt ccaaaagatg
    21121 gaccatcagc tggtataact atggctcttg cagtaatctc agcacttact aaaagacctg
    21181 tgcctggaaa tatagcaatg acaggagaga taactttaag aggtagagtc ttagcagtag
    21241 gaggagttaa agaaaagtta ttagcagctc atagagctgg tataacaaaa gtattaattc
    21301 ctaaagaatg tgaagctgat ttagatgaaa taccagaaaa tgtaaaagaa aaaatggagt
    21361 ttgtgcttgt agaacatatg gatgaagtat tagaacaagc tttattaaag agtggtgaaa
    21421 ataatgaaaa ttagaagttc agagataact atgagtgctg tgaataaatc acagtatcca
    21481 gcagaaggaa tcccagagat agcattggct ggtaggtcaa atgttggaaa atcatcaata
    21541 ataaatacat tgcttaacag aagaaacttc gctagaacca gtcaaacacc aggaaaaact
    21601 agaactataa atttttatct aataaataat gaattttatt ttgtagacct tccaggctat
    21661 ggatatgcaa agatagctaa gtctgaaaaa gaaaagtggg gaggtataat ggaacgatat
    21721 ctagaaagta gacaagagtt atgttctata ttcctacttg tagatattag acatgagcct
    21781 acagcagatg ataagcttat gtatgaatgg ataaagcact ttggatataa ctgtgtagtt
    21841 attgcaacta aggcagataa aatatctaga ggacaatatc aaaaacacat aagtataata
    21901 agaaaaaaat tgcaaatgga aagtagtgaa aaagtaattc ctgtttcttc actaaaaaag
    21961 actggtgttg aagaattatg ggaagagatt gtaaatcaat acaatcaaca tggatatgaa
    22021 ataacagtag attaaattta aaatgttaaa tattgacatg agatttattt gaataataaa
    22081 tgtaataata gttagtttta attatattaa tattaaagca aaatatagga tgccttaaaa
    22141 tagttttaat gaactttttt tggcatcctt attgtttata aacaataaat atcagtaatt
    22201 ttgttaaata ctaataaatt ttagtaaggt atgggaaaat tagtataaat tagttcaact
    22261 aaaaaattat tgacttatgt agtcagcaat aaatagcatt taagaaatag atttatctgt
    22321 ttttctcata aagaaaacag ttagtataag ccctataaaa catactgatg ctgcaatcca
    22381 ataagactga ttgctagcta taacataggc tgatgtgtga cttttaaact tttgattgta
    22441 tgtaagactt tgtgcaaaaa aactactaaa aaatgctgtt ccaagagcaa atgataaatt
    22501 acgaatagtt gcaagaaaac tgcctccgta tccctgtttt tgaggaggaa catctgctaa
    22561 aatactacta ttgtttggtg aactaaatgc tcctagccca agtccaaaaa taactaaaca
    22621 tacaattaaa agatacaatg gagaatctgc ttttaaaagt gccattccaa ctagtgagat
    22681 ggtcataaat gaaaaagatg ctgttagaat gttttttgca ccgtgtttat cagaaagtga
    22741 acctgcaata ggtgctataa gtaccataat aagtggatgt acagtcatta cataaccagc
    22801 catcatagga gataaatttt ttaattgttc taaataaaat ggaagtagaa aagatactgc
    22861 catttgagga aaataacaag ctactccaat gatattgcct acagtaaagt ttctattttt
    22921 aaatagaggt aggtcaataa agctttgttc aaattttact tctcttaatg ccaacaataa
    22981 gaagattata agtgctgtta tacctaaata caatcttgta ttacccttct cattcattgc
    23041 aagtaatata agcgtagtgc aagatgcgaa aagtagaaga cctggaatat caaagctctt
    23101 gttttgttca tccaaaacag gtttaggtag aaatttaatg ccaagaaaga ctgctataat
    23161 accaataggt atattgataa taaaaataga aggccatcca aaatgttcta aaattatacc
    23221 tccaattaca ggaccactca tatttcctat accaactaca gctccagtga ttcctatagc
    23281 ttttcctttt tcatttgctg gaaatgcatt ggaaactata ccaagcccag tagccattaa
    23341 aatacttgct ccaactgcct gaaagattct tgaagaaatt aatgttgata aattgtttga
    23401 catactacac agaaaagaac ctattgtaaa tataaaaaat cctaatgtgt atagacgatt
    23461 tgaacctact ttatcgccaa gttttccgaa aaataacata gtagctgtta caacaagcat
    23521 ataagctgtt acaacccact gaatttgtga catgttaatt ttaaagtcag cagcaattgt
    23581 aggatttgcg atatttagta tacttccatc aagagtagac ataaatacac caatacagat
    23641 gataatcaat actatccatt tttgtttcat gataacactc caatcttaat agattttata
    23701 agtacgatat aattgtaaaa taaacaacaa aaaaagcacc tgtatattag gtaccttgaa
    23761 tgtgtctaat ttcatgaata aatacggtag ttgaaggatt tgtatagatt actcatgtaa
    23821 aattacataa aggtactcat atactatttt cttctaccat ccagatttta ctgtcggctt
    23881 tggaatatga ccaaattatg ccataaggct cgcgggcttt taccgccgat agggaatttc
    23941 acccgtttat gaagaaaaac agatgtattt tgtaatatta ttttaacata aaattataat
    24001 ggaaacaatt ggatttaagt aatatccagc tgtattacaa tacaaatatt tcaatatgga
    24061 cacttaaatt gtatttatag atagaataag gaacagtatg agaatctata ataattagat
    24121 aaaattaacc tgttattagt attagatggt aagttttaaa tttatcaaaa tcagaaagct
    24181 gtaataaaat tcttataata tgaatatctt aattaagaga tattgtaaat tatgaggagg
    24241 cgctaatatg gaaggaaagg taaagaaggc attcccgggt ggaaatacat cacaaggctc
    24301 atattctttt tttgactata taattcctga aaatgtaaaa agagtttttt gtttaaaggg
    24361 tggacctgga gtaggaaaat cctcacttat gaaaaaaatt tataaagagt ttttgcatag
    24421 aggatatgat attgatttat atcattgtcc ttctgaccca agttcgttgg atggtttagt
    24481 tataaagaaa ttaggcgtgg tacttatgga tgcaactgcc ccacatacta tggacccaaa
    24541 gctacctgga gctttggatg agataattaa tttaggtgat tactggaata cagaagaatt
    24601 agaaaagaat aaacaagaaa tatcagttta tgataaagat attagtaatt catttaaaag
    24661 agcatttaag tttttaaaat cagcagaacc aatctttaga gacatagaag aaaaatattc
    24721 tgattgtatg gattatggaa aagtaaattt ggtcacagaa gaatttataa aaaggttatt
    24781 tgatggagtt aaaagcacag gaaatctaaa aagagaaaag catttatttg gttcagctat
    24841 aactcctgta gggtgtttag attatacaga aaacatttta aaagatgtaa agaaagtcta
    24901 ttatttagat ggagaaatag gaactgggaa aactacatta ttaaacaaag tatataagaa
    24961 agctattgaa aaaggtttaa atgtagaagt ttatcattat ccacttattc cagagaaaat
    25021 tgaaactgta ctattaactg atttagatat aggcataact ataagtacga cttttaaaga
    25081 agaagataca attgatttaa atcagttttt aaatagagaa aaactactta aatatgaaga
    25141 agatattaaa tttgatgaaa aagttttaga tgatttaatt tgttgggcag tactaaatct
    25201 taagagagcc aagtctaaac atgatattat agaaagttac tatagtccta atatgagatt
    25261 tgatgaagta gggaaattaa gagataagtt aattaaaaga attttaaaat atgaggaaaa
    25321 cttataaacg catatgttta aaattaacgt tagtaataat ataattttta gtaaattggt
    25381 gtagctttga ggctatgcca ttttactttt tttgctaaaa aatgatatta ataatctcca
    25441 aagccattat ttgtttttca taattattat ttcctttcct aatttatgtt tttttataat
    25501 atcacaacaa tgtttattga aagtttatat aattttaaga acatctaaaa atgcatactt
    25561 gatattggta aattagtgat aggagttcaa tgtatgatat tatatactaa agatgaattt
    25621 taatatataa ctttgatata ataagcatat gtaaatgttt aatatttaat taaagaaaat
    25681 tatcatataa aaataaattt gtaaggagca atacagtatg aaaaaacaat atgtgtttat
    25741 aatactagca agtactattt taggtatact tgttggaacc atatttagag attatagcaa
    25801 tgaagaaaca gtttatacaa gcaaagacaa gtcaataaga aaagaaataa aaatgacaag
    25861 aaaatctata aaagaattaa ccaaagagaa agacaagtta gataaagaaa ttaattcaat
    25921 gaaacaaaat caaaaaaatt ctgaagaaat atcagaaata gaaacactta gagatagtct
    25981 atcatattca gatgtaaagg gtagtgggat aatattaagt atagatgcta tagatgaaaa
    26041 aacagggaat atagctaata gtatagacta taataagatt cttgtaaaag ttataaatga
    26101 attaaaagta aacggaggaa aatacataga aataaatggt caaagaatta atcaatattc
    26161 agaagttgta cttgcaggaa gccatataaa tataaatgga gttcctgtag cacaaccata
    26221 tgaaataaag tgcattggag ataaggagaa actatctaat tatgtagata aaggtagtaa
    26281 atatttagat agcatacaaa ccaattatcc aatgaaaata agcataaaaa ctgaagaaaa
    26341 tatctctatg aaaaaaatga atatacctaa taaattaaag tatataaagg gtgattaaga
    26401 ttatattaga ggaaagcagt ttgccaatgt taataccaga aaaacttgca aaaaaatata
    26461 atgtatttcc agttgagttt caagatgata atttagttgt agaaatagaa gatgaggata
    26521 tttatgctct acaggacttg agattagcta caggtaaaga aattatattg aagaaggaga
    26581 aaaaagaact tataagtgaa aagattgaaa aatattataa tagctttgaa cttgatgagg
    26641 attatgcaaa aaaattattt gaaaatcttt tagaaaaagc tataaaagaa aacgcaagtg
    26701 atatacatat agaaccattt aaagaacatt taatagtgag aatgagggta gatggagaac
    26761 taaaggaagt atcaaagttt ttagttgatg tctatccatc attgtctacg gttataaaat
    26821 taaaggcatc aatggatata acagaaaaaa ggcttcctca ggatggtagg gtggatataa
    26881 agttaggtgg aaatttaatt gacattagag tttcatcaat accaactgtc tatggtgaaa
    26941 aaatagtttt gaggatactt aatagagata cttttttaaa agacaaatta gaacttggtt
    27001 tttcagagga agctataaaa tctataaatc gtataataaa taaaagagca ggaatattat
    27061 tagtgacagg tcctacaggt agtggaaaga ctacaacagt atattcctta ttaaatgatt
    27121 taaaaggaat aaataaaaat attatgacta tagaaaatcc tgtagagtat aagatggatg
    27181 gcataagtca gattcaagta aattcaaaag tagggttgag ctttgatgta ggtcttagag
    27241 cgatattaag acaagaccca gatataataa tggtcggaga aataagagat gcagaaacag
    27301 ctaagatagc agtaagagca gctataacag gtcatttagt tataagtact ttacatacta
    27361 atgatgcagt atcttccata gctagattac tagaaatgca aataccacca tatcttttaa
    27421 atgcctctct tataggtgtt atatctcaaa agttggttag aaaagtctgc aatcattgta
    27481 gccatgagat tatgataaaa gataatttta gtggagatgt aaatactaag gtagctgtag
    27541 ggtgtgaaaa atgtaatgat aaaggatact ttggaagaac agcaatttat gaaatcttag
    27601 aaattaatga tgatataaag acatgtataa gaaatatgga ggattctagt actattaaag
    27661 atgtagcaca aaataatgga atgataacat ttgaagatag ttgtaaaaga ctaataaatg
    27721 aaaaaataac gacattagaa gagtgtttag tagtaaatga aatgatttaa ctgaattgta
    27781 tagttaaact tcgtttggtc agttgtcttt atttaatcta ttttaattaa gatgtttgag
    27841 tataaattat atttaagtga aataaaatta aatgcattaa attagaaaat attaagttta
    27901 tttaattgta attaatataa tttggaaggt attatagtac atgagattat ctaaaaatat
    27961 attttataat ttagaaaagt tttatttagt attaaataga atcagtgata aagaaattag
    28021 agtaatttgc aaagagatag gcatattgct ggaatctgga tgtgaaatta caaaaatatt
    28081 tgaaatcatt gagagtcagt caagtaaaaa agtaaagaac ttgcttagta tagtatcaaa
    28141 tcatattcaa aaaggtaact caatagcaga atcatttcaa ataacaggta tattttctaa
    28201 attctttata agtatgataa aagcaggaga aactagtgga aacttagata taataatgag
    28261 tgatttatct aattattatg ataaagaata caaattaaaa atgaaaataa taactatttc
    28321 aatatatcca ataatactta taatattatc tatcttatca atgttgttta tattcgtatt
    28381 tgtaatacct aattttcagg tggtttttac caacaatgga attgaaccac ctttaataac
    28441 tagagtttta atgggaatat ctacagtggt tacaaataac ttagcttata taatatttag
    28501 ttttatattg ttttcaatag gaagcattta tttctttata acaaatgata atataaaaaa
    28561 attgattaat tcgttaaaat tcaaaatacc ttttataaaa aaaattaatc agttagtagc
    28621 tacaacaaga ttttcaaggg cattgtccgt tttaattagc agtggaatac aaattgtgga
    28681 agctatagat atgtcagcta gtgttataga caacgaattt atttatgaaa ggttaaagat
    28741 ttctaataat tatataaaaa aaggaaatag cataggtaaa tctttgaatt tagcaaatat
    28801 ttttccaaaa ttatttattt caatggcaaa tacaggagaa gaaagtggga aactagataa
    28861 aagtctaaat gtaataaata aattttatga aaatgaatta aataacaaaa tagagcaaat
    28921 aatgaaattt atagaaccta tgctaattgt atttatggga ttaattattg gtacttttat
    28981 tttagcaatg gttgttccta tgtttgatgc aattactaat ttttagagag aaaaataaat
    29041 tttattagat gaaacttttg aaaaggagag aatatttaat tgataaattt gataaataaa
    29101 aaacgaaaag gatttacact tgttgaaatg attgtagtag taactatttt aggcgttata
    29161 tctagtatag cattagttaa gtatagtaag gttcaagaaa gtgccaaatt aaatgcagac
    29221 tatacgaatg ctgctaatat agtaaccgca gctagcatgg caattaatga tgatgaaaag
    29281 acaatagact ctctaagtgt agagacattg aaggaaaagg gatacctaaa tactgttcca
    29341 gttcctcaga gtacatcagg taaattcgaa cttgtcataa atgatagcgg aacagatata
    29401 agcgtaaata taaattctaa gcaattttat ccaaaataat attttatatt gattgctgta
    29461 agttttaaaa ttatttttat ttaagaagga gatggctata attaaaaaaa ttctaaactt
    29521 agaaataaat gatgattata taaggcttgt atttttaaga aaaagtcata aaaaagtttt
    29581 tatagataaa tcaattataa aagagattaa ctttgatata aaaaatgata gtaattcgat
    29641 tgagaagatt aaatatatag aagatgtagt tagatttgta aaagaggaga ttggtaaatc
    29701 aaaaatatta tataaaaatt tatactttaa cattcaaatt caggatatag tcattagaaa
    29761 tgttgaagtt ctaaatacta agaaaaaaag agacatatta agcatgattg aatttgaaat
    29821 aacacaatac attccaataa acataaataa ctattcaata aaatataaag ttataaattc
    29881 taaaaatgat aaattgaata tacaagtaat tcttttccca aagaatatta ttcaattaat
    29941 taagaacatt tcagaaaatc taaatatgaa tccaaagact ataaatataa attttgatat
    30001 attacaaaag cttataaact taaatttaat agagaatttt tcagaagaag gggtatttat
    30061 agaatgtaaa aaaagtcagt taattataaa tataacaaaa gataaaaaaa taagagaaac
    30121 atacttgcta tcaaaagagg gaaaatcata tgagttcata aaaagattat taaggggttt
    30181 taagtatgtt tattactatg gtatagaaaa ttgttttatt gaagaatact ttaaatatac
    30241 agataaatct attgaagtaa atccattaag attaaaagat aaagtaaaag tagaaaataa
    30301 ttttgatgca gatttagaag acaataggat aaattatata aatagtatag gaatgattat
    30361 ttaagtgaaa gtggataaaa caataaattt tcttaatgga tttaaaaaaa ctaaaccatg
    30421 taataaaatc tataaaataa taatagtctt aattttaacg tgtattttat ttaaatcatt
    30481 tatgtatgtg aaatctataa atgaacttga agattatata agtcaaaata agctagtgga
    30541 taaatcaaag aacactttac caacaacaga taaaactaga gagaaatttt ctaaaataaa
    30601 acttaatgaa ataaaaaaaa tatatgaaac aattggaata aatagtatta ctaaaatatc
    30661 aatagataaa aataagctag agatggaagg tgtgtgtagt gatttagatg ttttaaacaa
    30721 tttaaggaaa ataaagtaca taaaatatta ttacatagac agtattgtaa gagatggaga
    30781 atattataga tttagaataa aaagttagat gggtgattat ttttgagatt aaataataga
    30841 gaaaaaaatc tacttaaaat attgttaata ttagttatta tattggcaag tgatatagca
    30901 gtaagttatt tggaagaaaa cctaactagt ttgaagaatg aaaagcaaaa tataataaat
    30961 aaaataaata atcaagataa ttatcaaata aaggataata agcatataga tgataaatat
    31021 atagaaagtg ttgaattaaa taaaaatgaa gaattgaaaa ataattttaa taactcattt
    31081 ttagataaaa aaacaaataa agatatagtg ttattagtag aaaatgaact gagagatatt
    31141 gtgaatgtag aatatattaa tcaagaaagt agtttagata atcacaataa aaaacaagtt
    31201 aatttagaaa taaaagttaa tggagaactt gcaaatgtgt tgaaacttga aggtgtaatg
    31261 aagaagttaa gactaaataa taaacttgaa aagatagaaa ttaacaaatt aaaagtcaat
    31321 aattttgatg ttcaaaatat tccaaatgaa tataaacaag aaaaagacaa tattgttgaa
    31381 tgtaaaatga cagttagagt catataacaa aaaagaatag ataaagtaaa aaactattat
    31441 caattaataa aattttatag aggaattagt tttacgataa ttcatttcta caagagggat
    31501 tgttgatgat tttatcgaaa tcctaattaa agttaatata ttgtaccaaa atataagtga
    31561 atttaataga atatttactt atactataga ttgattaata aaaaaatggt ataataacaa
    31621 attgtacttt aatttaataa taagggagtg gaagtcatat tgttagggag aaatgagcta
    31681 tgcccttgtg gaagtggcaa aaaatacaag aaatgttgtt taaataaaga tattgtttca
    31741 gaaagagcat gcagaaaaac tgaactttca caaaaacaat atacagattt atattcaaag
    31801 ttatatgatt attcaagaca ggataagttt aaagaagaat gtgaaaaagc taaagaaatg
    31861 ttttatataa tgcaaaatga tagtgtaaat gaaaaatttg aaagattttt taatacttat
    31921 ttaatacaag accacataat ggaaaataaa aaggttatga cagttggttt tcttgaagaa
    31981 aatggtgcaa acctaagtca atcagaagta tcaatattaa aaagtatgtt tgaatcatac
    32041 gtaagtattt atgaggtaaa agaagtttct gctggaaaaa tagtattaaa ggactgttta
    32101 aatggaaatg aactttgtac agaagatgta aaacttctga gaagttttaa aatcggaagt
    32161 tgtatgatag ctagaatagt tgatattgaa ggaactaaca tattgataga tataactata
    32221 agtatatcaa atgaggttaa agatataatt ttaaaggata ttatgaatct attcaatcaa
    32281 tatcaagatg tatacaaaga tatgaagtcg ttcttaattt atcatacaca tatactatac
    32341 aagtatatac aacagctttt agacccaagt atagctgaat atttaaagag agaaagagat
    32401 aaaaacgata caaaagttaa ttcagaaaat gaattagctg aagaagaatg taaagtttta
    32461 gatactctta aagaaaatgt agaaaaagaa aatatagatg catgtataga gttttggaat
    32521 gaatataaga aaaatcatga aaatataaaa ggagctgaaa atggttgggc agctgctgtt
    32581 gaatactatg ttaaaaaaga agcaggacaa cctataactc aagttcaaat atcaaagaaa
    32641 tacgatataa gctctagtac actaggaaag agatacaagg atttaaaaat atcttaatta
    32701 attataaaaa gctttaagtc atagataaag aatctatagt ataatatatg tataatgttt
    32761 agaaagaggt attaataatg agtcttagtt taggaaatgc aattgctgta tcaacgaact
    32821 ttataaaatc taacccagct gtacctgtgt acaacggaga agaaatatat gcagttattt
    32881 atcatataga aataggatat aataatggtg tatcacctga taccaaacaa tatataataa
    32941 tagactataa agagtttgat ttatctatgg ataaaaaaaa tgaactaata aatattgcta
    33001 aagagtactg taaatctaaa ggtattgtag aaaacgaaaa ccttgaaata aaccttttag
    33061 acaatgaaga agcagagaac ttcttagaaa aggtgtttaa taatatgaag gttataagag
    33121 gtcttataac taaaaaagga taaataaatg gactatatag gaatagaaaa tataacacct
    33181 tatgaaaata catatgaatt tagtgtatat gaatatgatg atgaaatcac cttaggtagt
    33241 gaaaagttat atgtatgtga attaagggtt gtattgatta aagttaattc tctgtatgtt
    33301 gaaagattgc ataaatcagt tgaagcaatg gtcttagtaa aaaacttgaa aaaagattta
    33361 gataaaacac ttgttgtaaa caaaataaag aattttgtgc tagatgagat ttgggtagaa
    33421 aatctagtaa aagagaatat agaagttata tttgtagaaa gctaggaata accctagctt
    33481 ttttatatta caatctaaga gaggaaaaca atatgttaag agtatctaat gtaaaacttg
    33541 gaattgataa agatatatct ttattaaaag atgttatact taagaagcta aaaataaaag
    33601 aaaaaaactt agtcaaatat agtatatata aagaatctat agatgctaga aaaaaaggaa
    33661 aaatggaatt tgtttattct gtagatgtag aagttaaaga tgaaaataaa atacttaact
    33721 caaaaataaa agatgtaaca aaaataaagg aaatcagata cgtaaatgta cctgtgggtg
    33781 ataaaaaatt aaagaataat cctttggtaa ttggtagtgg accagctggc ttgtttgcag
    33841 gtctgctttt agctcaaatg ggttatgaac caatcatact tgaaagaggt cttgatgtag
    33901 acagtagaac caaagatata agtgattttt ggacaactag aaaattaaaa aataattcaa
    33961 acgttcaatt tggagaaggt ggagcaggaa ctttttcgga tggtaaactt acaactagaa
    34021 taaaagatat acgatgtaga aaagtattag aagagttagt tcagtttgga gcaccagatg
    34081 aaatattata ctcacataag ccacatgttg gaacagatat attaaaaaat gtagttaaaa
    34141 atataagaaa tgaaataata agtttaggtg gtcaagttag atttaattca aaagtaacag
    34201 atatagttgt atcaaatgat tctatacaat ctgtaataat aaatgatact gaaaaaatag
    34261 atacagatac aattatatta gctgttggac atagtgcaag agatacttat gaaatgttgt
    34321 attcaagagg tataaaaata gttcaaaaac cttttgcaat aggagctaga gttgaacatc
    34381 cacaagattt aataaataaa gcacagtaca aggattttta taatcatcct aggttaggag
    34441 ctgcagatta tagacttata gagcatgttt caaatggaag aacagcatat actttttgta
    34501 tgtgtccagg aggtagtgtc atagcatctg catcaaatga ttttgaaata gttacaaatg
    34561 gtatgagtga acatgcaaga gataaaataa atgcaaatag tgcattttta gtaaatgtaa
    34621 cgccagaaga ttttaagagt gataatccac tagcaggtgt tcatttccag cagaagtatg
    34681 aaaaattagc ttttgaatta ggtggaaaaa attataatgc accagttcag cttgttggag
    34741 attttataaa caataagact tctttgaatt taggaagtgt tgagccatca tatagaccag
    34801 gatatacact tacaaattta agtgaatgtt tgcctagctt cgtaacagag actatgagag
    34861 aagcgtttat atctcttgat aaaaaattga atgggtttgc aatgtatgat gctgttttga
    34921 cgggagtaga aactaggtca tcatcaccaa taagaatagt aagagatgaa aatactttag
    34981 aatcagtgag tattaagaat ctatatccat gtggtgaagg tgctggatat gcaggtggta
    35041 tagttacagc agcagttgat ggtataaaat gtgcagaaaa aataatacaa aaatacacca
    35101 ataatacaaa aatttaacat aaacttaatg attttttaat atttaacatg aatttaacat
    35161 tatcactcct ataatttgat aaaatacaat aggttataaa atttttagat acagtaatct
    35221 ctttacaagg aggaaataat ttgaacatta taataagtct aatgggtgga ttaggtctgt
    35281 tcttatatgg tatgaacctt atgggagaag gtcttcaaaa atctgctggg acaaagctaa
    35341 aaaagataat aaaactactt actagtaacc tatttatggg agttttggta ggtacaggag
    35401 ttacagcagt aatacaaagt tcaagtgcta ctactgtaat ggttgttggt ttcgtaaatg
    35461 caggaataat gactttaaaa caggcaatag gagttataat gggtgctaat atagggacta
    35521 ctgttacagc ccaattggta tctttcgact taactggtat ggccccagta gcattaggtg
    35581 taggtattat actttattta tttggaaata agcctagaat aaaaaatata gccgaaatac
    35641 ttataggttt tggtatatta tttacaggta tggattttat gaagatggca gtggagccac
    35701 taagagacta tcaaggattt actgacttac ttgttacatt tggtagatat ccactacttg
    35761 gattattatt gggatttggg attacagcaa taatacaaag ttcaagtgca tctatgggta
    35821 tgttggttgc actggcagca gaaggtctag tgccattatc agcagcactg ccaattcttt
    35881 atggccaaaa tattggtaca tgtgtaactt cactgttatc aagtattggt gctaacaaaa
    35941 atgcacgaag agcagctatg atgcatctaa tcttcaatgt tttaggaacg gtgatattct
    36001 taatattctt gaataaacca gtagtaagca tggttacaag ttgggaccca agcaatgttg
    36061 caagacaaat agccaataca catacattgt ttaatataat atctgtattg atacttttac
    36121 catttacaaa tcttattata aaactagcta taaagcttgt tcctgataga gcaggtgata
    36181 tagatgaaga tgaaactaaa actattaaat atatagatga tagaatgata gaaactcctt
    36241 ctatagctct tgcaaatacg ataaaagaag cacttcgtat gggtgagaag gcgaaggaaa
    36301 gcctaaatgc atctatggaa gctttagtag aacattcaac tgaaaaaata gataagacat
    36361 ataggcgaga gagattaatc aatgatttac agaaagctat tttaaattat ttacttaaac
    36421 tttcaaaagc acctttagat gatgattcaa gagaagttgt ggatactttg tttaatacag
    36481 tcaatgatat agaaagaatt ggtgaccacg ctaaaaatat agctgaatta tcgcaagttg
    36541 caatagattc aaatatttct ttctcagaag aaggacaatc tgaacttgat gttatgtata
    36601 atagagttgt ttcagcatat acttatgcat tagaatcaat gagaactgat aatgttgatt
    36661 tagcttgtaa ggttataaaa attgaagaac aagttgatat aatggagaaa tcttgtagag
    36721 ctaatcacat gtatagattg aacaataatc tatgtagtat agaaaatgga gttatttatt
    36781 tagacgtaat aagtaattta gagagaatat ctgaccatgc tgtcaatata gcacaacaag
    36841 ttatagcaaa gagattagga aatgattaaa aataattaat ttaaaataat ataaaattta
    36901 aaatagagga taaataaaag agaaacttta aatgatacgt ttagagtttc tttttttgta
    36961 aatttatttg actaaaattt ttaaatttat acatagttat agcatttaga agaatcgatt
    37021 tcctgataat aaatgatgag atatgtatga ttttgtggca aacttttaaa aaaaacacct
    37081 atattttgtg ttataatctc tttaaaacga atatgctgaa aagagggatt taaatgggaa
    37141 aaataaattt tgattatagt aaagctacag agtttttttg tcaaaacgaa atagatgtca
    37201 tgcaaccata tgtagatgtt gcgcacgaca tgttacataa taaaactggt ttaggaaata
    37261 cttttttagg atggatagac cttcctaaaa attatgataa agaagagttt gacagaataa
    37321 aaaaatctgc tgataagata aaatcagact cagatgttct tttggtaata ggtataggag
    37381 gctcttattt aggttcgaga gctgctatag atatggtaag tcattctttt aggaatggac
    37441 ttaaaaaaga gcaaagaaag gctccagaag tatattttgt agggcataat ataagctcaa
    37501 catatattat ggatttatta gatataattg aaggaaagga tatttctgta aatgtaatat
    37561 ctaaatcagg aactactaca gaaccagctc tggcttttag aatatttaaa gattacttag
    37621 agaaaaagta tggtaaggaa gaagcaagaa agagaattta tgcaacaaca gatgcaagta
    37681 aaggtgcact tagacagtta gctacagaag aaggatatga gacttttgta ataccagatg
    37741 atgtaggtgg tcgtttttct gtattgacag cagttgggtt acttcctatt gctgctgctg
    37801 gacttgatat tgatgctatg atgaaaggcg caaatgatgc tagagaagca tttcaaaatc
    37861 ctgacttaaa aagtaatgat agttatagat atgctgttgc tagaacaatt ttacatagaa
    37921 aaggaaaaga tgtagagtta ttagtgaact atgaacctca actacattat gtaagtgagt
    37981 ggtggaaaca gctttatgga gaaagtgaag gaaaagaaaa taaaggttta ttccctgctt
    38041 ctgtagattt ctcaacagat ttacattcta tgggtcaata catacaagat ggaaaaaggc
    38101 ttttatttga gacagtactt aatgttgaaa actgtaagag aaatataact ataagttcag
    38161 aggaagtaga cttagatggt ttaaactatt tagcaggtaa gactgtagat tttgtaaatc
    38221 ataaagcttt tgaaggaact ctattggctc atacagatgg aaaagtacct aatttagtaa
    38281 taaatatacc tcaactagat gagtataatt ttggatattt agtttatttc tttgagaagg
    38341 cttgtggaat aagtggatat ttattgggag ttaatccatt tgaccaacca ggagtagaag
    38401 cttacaagaa aaatatgttt gctttactag gcaaaccagg ttatgaaaaa gaaaaggaag
    38461 agctagaaaa aagacttaaa taattttaaa tagttaaggt ttaattaaga ttaaaataat
    38521 ataatgaaat catcgaaaag gagtttactc aaaatagatg atttcatttt ttttatgaaa
    38581 aattgggaaa aatatatttt gataatttat attatattaa gatagggagt ggtatgagat
    38641 gtcaagaaga aagaaaggga taagtttagt aatattagtt gctataataa gttcaatact
    38701 tagtagtttt ctaacaataa ttttagttaa ggacaattta gtaagtaaat ctacaggaag
    38761 ttctacgccg atagttgtaa atgatgatgg taagagtcaa aatatttatc aagcagttgc
    38821 agaaaaagct acgccatctg ttgtaggtat tacaacaact agtgttgata ctagtaatat
    38881 gtttgcaata ccaactgaaa cacaaggtgt aggaactgga atcatagttg attcaaatgg
    38941 atatatactt actaattctc atgttatatc agatggtcaa gctacaagtg taaatgtatt
    39001 attcaatgat ggttcaacaa cttcaggtaa agtagtatgg tttgaccaac aacttgattt
    39061 agctatagtt aaggttgata aaactggact aacaccagca gaatttgctg atagtgacaa
    39121 agtaaaagtt ggagatatat caatagcaat aggtaatcct cttggattag attttcaaaa
    39181 aacagttact caagggataa taagtggatt agatagaact atacaaacag aaaaaactaa
    39241 tatgactggt ttattacaaa ctgatgcaag tataaatgca ggaaatagtg gaggaccatt
    39301 gttaaatcaa aaaggtcaag taattggtat aaacacagca aaagcttctc aagcagaagg
    39361 attaggattt gcaataccaa tcaatacagc taagagtata gttgaagaag taatcaaaaa
    39421 tggaaaatat gaaaaagtaa ctttaggcat aaaaggtaca gatgtatcaa attatgaagc
    39481 agcaacaggt acaaaattaa gtactgataa aggtgtttat gttgcagaag taatatctgg
    39541 ttcatcagca gaaaaagctg gagtaaaggt aggggatatt ataactaaag taggagatac
    39601 agatataaca ggtatgaatg atttaaataa aaagttgtat actttctcaa aaggtgctag
    39661 tacaaaaata acagtaaata gaggcggaaa agcagtaaca ataaatgtga atttctaaat
    39721 atgaccagaa agatagagac tacatggtaa agtgtagtct ctatttttat ttaattttta
    39781 tagtatatac atctaaatta attaaaatga tgta
//
LOCUS       DAYAOX010000011        39219 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.011, whole genome shotgun sequence.
ACCESSION   DAYAOX010000011 DAYAOX010000000
VERSION     DAYAOX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.011"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            54..650
                     /locus_tag="ACGI57_000457"
     CDS             54..650
                     /locus_tag="ACGI57_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFG9715545.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            680..1399
                     /locus_tag="ACGI57_000458"
     CDS             680..1399
                     /locus_tag="ACGI57_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFG9715546.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            1401..1940
                     /gene="scpB"
                     /locus_tag="ACGI57_000459"
     CDS             1401..1940
                     /gene="scpB"
                     /locus_tag="ACGI57_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFG9715547.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            2171..2731
                     /locus_tag="ACGI57_000460"
     CDS             2171..2731
                     /locus_tag="ACGI57_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HFG9715548.1"
                     /translation="MLILISHINILIIADIKNKDICLKLNIKYMFNLININRQLYPAE
                     NSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNINFGTGNIGLTSSVY
                     VLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIKALFNIIIMINKIM
                     NKNKGNKEGDSNESNRFDTESYGNNS"
     gene            2715..3113
                     /locus_tag="ACGI57_000461"
     CDS             2715..3113
                     /locus_tag="ACGI57_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HFG9715549.1"
                     /translation="METTLETIKGSIDANTIIGDPIKTDTTVVVPISKVTIGFGIGGG
                     EYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETRIMSLDSNINL
                     VDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            3361..3801
                     /locus_tag="ACGI57_000462"
     CDS             3361..3801
                     /locus_tag="ACGI57_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9715550.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDINNKDKLFTSLEESKNIIEQLF"
     gene            3817..5268
                     /locus_tag="ACGI57_000463"
     CDS             3817..5268
                     /locus_tag="ACGI57_000463"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFG9715551.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALILLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(5359..6027)
                     /locus_tag="ACGI57_000464"
     CDS             complement(5359..6027)
                     /locus_tag="ACGI57_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HFG9715552.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
     gene            6296..6436
                     /locus_tag="ACGI57_000465"
     CDS             6296..6436
                     /locus_tag="ACGI57_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715553.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(6611..6931)
                     /locus_tag="ACGI57_000466"
     CDS             complement(6611..6931)
                     /locus_tag="ACGI57_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFG9715554.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(7051..7788)
                     /locus_tag="ACGI57_000467"
     CDS             complement(7051..7788)
                     /locus_tag="ACGI57_000467"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFG9715555.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            8003..9826
                     /gene="acd"
                     /locus_tag="ACGI57_000468"
     CDS             8003..9826
                     /gene="acd"
                     /locus_tag="ACGI57_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HFG9715556.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISEGNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            10041..14339
                     /locus_tag="ACGI57_000469"
     CDS             10041..14339
                     /locus_tag="ACGI57_000469"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFG9715557.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            14543..15004
                     /gene="rimP"
                     /locus_tag="ACGI57_000470"
     CDS             14543..15004
                     /gene="rimP"
                     /locus_tag="ACGI57_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFG9715558.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            15034..16188
                     /gene="nusA"
                     /locus_tag="ACGI57_000471"
     CDS             15034..16188
                     /gene="nusA"
                     /locus_tag="ACGI57_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFG9715559.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            16195..16476
                     /locus_tag="ACGI57_000472"
     CDS             16195..16476
                     /locus_tag="ACGI57_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFG9715560.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            16466..16777
                     /locus_tag="ACGI57_000473"
     CDS             16466..16777
                     /locus_tag="ACGI57_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HFG9715561.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            16799..18739
                     /gene="infB"
                     /locus_tag="ACGI57_000474"
     CDS             16799..18739
                     /gene="infB"
                     /locus_tag="ACGI57_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFG9715562.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            18782..19162
                     /gene="rbfA"
                     /locus_tag="ACGI57_000475"
     CDS             18782..19162
                     /gene="rbfA"
                     /locus_tag="ACGI57_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFG9715563.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            19143..20126
                     /locus_tag="ACGI57_000476"
     CDS             19143..20126
                     /locus_tag="ACGI57_000476"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFG9715564.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            20139..21044
                     /gene="truB"
                     /locus_tag="ACGI57_000477"
     CDS             20139..21044
                     /gene="truB"
                     /locus_tag="ACGI57_000477"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFG9715565.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            21175..22104
                     /locus_tag="ACGI57_000478"
     CDS             21175..22104
                     /locus_tag="ACGI57_000478"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFG9715566.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            22208..22465
                     /gene="rpsO"
                     /locus_tag="ACGI57_000479"
     CDS             22208..22465
                     /gene="rpsO"
                     /locus_tag="ACGI57_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFG9715567.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            22705..23550
                     /locus_tag="ACGI57_000480"
     CDS             22705..23550
                     /locus_tag="ACGI57_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFG9715568.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            23675..25786
                     /gene="pnp"
                     /locus_tag="ACGI57_000481"
     CDS             23675..25786
                     /gene="pnp"
                     /locus_tag="ACGI57_000481"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFG9715569.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            26052..26795
                     /locus_tag="ACGI57_000482"
     CDS             26052..26795
                     /locus_tag="ACGI57_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9715570.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKAGKLSDVFK"
     gene            26953..28200
                     /locus_tag="ACGI57_000483"
     CDS             26953..28200
                     /locus_tag="ACGI57_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFG9715571.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINSVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            28235..28480
                     /locus_tag="ACGI57_000484"
     CDS             28235..28480
                     /locus_tag="ACGI57_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HFG9715572.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            28490..29713
                     /gene="dapG"
                     /locus_tag="ACGI57_000485"
     CDS             28490..29713
                     /gene="dapG"
                     /locus_tag="ACGI57_000485"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFG9715573.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            29826..30560
                     /locus_tag="ACGI57_000486"
     CDS             29826..30560
                     /locus_tag="ACGI57_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HFG9715574.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            30683..33094
                     /locus_tag="ACGI57_000487"
     CDS             30683..33094
                     /locus_tag="ACGI57_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFG9715575.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            33097..34155
                     /gene="mnmH"
                     /locus_tag="ACGI57_000488"
     CDS             33097..34155
                     /gene="mnmH"
                     /locus_tag="ACGI57_000488"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFG9715576.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            34149..35483
                     /gene="rimO"
                     /locus_tag="ACGI57_000489"
     CDS             34149..35483
                     /gene="rimO"
                     /locus_tag="ACGI57_000489"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFG9715577.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            35470..36012
                     /gene="pgsA"
                     /locus_tag="ACGI57_000490"
     CDS             35470..36012
                     /gene="pgsA"
                     /locus_tag="ACGI57_000490"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFG9715578.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            36300..37346
                     /gene="recA"
                     /locus_tag="ACGI57_000491"
     CDS             36300..37346
                     /gene="recA"
                     /locus_tag="ACGI57_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFG9715579.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            37522..39063
                     /gene="rny"
                     /locus_tag="ACGI57_000492"
     CDS             37522..39063
                     /gene="rny"
                     /locus_tag="ACGI57_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFG9715580.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT        15989 a         4066 c         7352 g        11812 t
ORIGIN      
        1 gctatttaat tgttataata atattaaaaa tttaatttag gagatgtaca ttaatgttaa
       61 gtatgaatat aggagaaata gtagcaagtt tagttggtat agctatggca atatcaatac
      121 atgagtttgg ccatgcttat tcagctcact tattgggaga tgatacagct aaagcttatg
      181 gtagaatgac attaaatcct gtaagacatg ttgacccaat aggtcttttg gctatgttta
      241 tccttaagat agggtgggca aagccagtac cagtaaatcc aaataacttt aaaaactata
      301 agataggaaa cctgatagtt tcacttgcag gcgttttttg taatgttttg actgctatac
      361 tgtgtgtaat tatcaataag tatgtgcata tgtatgctat aaatacaata gctgaatatg
      421 tgtttttata tagtattgga tttgcagctt ttaacttact tccgatacct ccacttgatg
      481 gatggggagt tatatctaca tttgtaccat ataaatggaa tgaatatttg tataaatttg
      541 aaagcatgag ttatataatt ttattaattg ctttatttac tggtatgtat agaataatat
      601 taacaccaat atatgcagtg ttgctaaaaa tagtatattt atttgtttaa tataataaac
      661 agattaaaga gggattgata tgaagtataa tatacaatta caagtctatg agggaccatt
      721 agatttactt tatgatttaa taacaaagca taaaatagat ataaaggata tatccataat
      781 agatataact aagcagtatc taaattattt gaaaatgcta gataaaatgg atttagagat
      841 aactagtgaa tttataacta tggcatcaaa acttttagag ataaagtcta aatatctgct
      901 ttataagcaa aaagatgaag aagaagaccc aagaatagaa cttatggaaa agttagaaga
      961 atatagaaag tttaaggtag catcacaaga tataaaagaa aatataactt atgttaatga
     1021 gagattttat agaaataaag aagagataat tattgatgac aatgtggatt tagaagatat
     1081 atcaatagaa gcaataaaaa atatacttcc atacatattt aaagttaaaa catcacagat
     1141 agaaaatgct aatgatgaga aactagataa aatagtaagg aagaaaataa tatctgtaga
     1201 agaaaaaata ttatatataa gagatattat aaaagagaag attgaagtta cttttacaaa
     1261 tattataaaa tcatatgaaa atgatgaaat aatagctact tttttatcta tattagagct
     1321 tataaaagaa aaagaaatag tcgtagtaca agatatattt tttgatgata ttctaataag
     1381 aaaaagttcg gagtgttaaa atgaaaagag aagatataaa gtatattata gaatctgtta
     1441 tgtttgcata tggtgaacca ataagtataa aagaattaaa ctatataata aataaagaac
     1501 tttcatcaaa agaaatagag attatgttaa atttacttat tgaggagtac agagaacaaa
     1561 atagaggtat tcaaataatc aagttagaga ataagtacca aatgtgtaca aataaagact
     1621 atgcagaata tataaagaag attattgaac caaagaaaaa gaaaagtttg agccaagcta
     1681 cattagaaac tcttactata attgcctaca aacaaccaat aacaaaggta gaaatagagg
     1741 atataagagg agtaaaatgt gataaggtat tacaaacttt gtttgaaaat gaacttataa
     1801 gagaagctgg tagattgaat aagataggga aaccaataat ttataaaaca acagatgaat
     1861 ttttaaagtt gttgaatata gaaagtcttg aagaattgcc acctatagaa aattatcaag
     1921 aagtggcaac aaatgaataa aaatataaaa tcacaataat ttagtatgta attttaaatt
     1981 ttaaaagatt taaaatgctc taagttgttg tgtttttttg tataaatata aaattataat
     2041 tctcttaaaa taaatatgga taattaagta taaatagtaa ttttttgatg ataatataaa
     2101 catggagctt aatgaattat ttaattttaa gtatttaata atagctgtaa tttttattat
     2161 aagtgtaact atgttgattt taatatctca tataaacatc ttaataatag ctgatataaa
     2221 aaacaaagat atttgtttaa agcttaatat aaaatacatg tttaatttaa taaatataaa
     2281 tagacaactt tatccagctg aaaatagtaa aaataatgat aaaaaagagg ggatgaagaa
     2341 taatatagat tcaagtatat tattagctga tgaccttttg agcatttata gattattaaa
     2401 aaagataaaa atacatgaac tttattcaaa tattaatttt ggaactggaa atattggctt
     2461 aacatcttct gtatatgtat tgataaatac tttgtatgga aatttattta atatgattga
     2521 tgcagaaaaa atgtatttaa atgtaaatcc agatttcaca aaagattatg ttttaggaaa
     2581 tataaggata catataaggc caagaataaa agctttattt aatattataa taatgattaa
     2641 taaaattatg aataaaaata aaggaaataa ggaaggtgat agtaatgaga gcaacaggtt
     2701 cgatacagag tcttatggaa acaactcttg aaacaattaa aggttctata gatgcaaata
     2761 ctattatagg agaccctata aaaacagata ctacagtggt tgtacctata tcaaaggtta
     2821 caattggctt tggtatagga ggaggagaat actcaaaagg atatgatgat aaagataggg
     2881 aaattgaatt aaaaaatgaa aaatcagata cgaattttgc tggaggtagc gcaggggcta
     2941 tatctgtaca accagtagca tttgtagtag ttgaaagtgg agagactaga ataatgagtt
     3001 tagatagtaa tatcaatcta gtggataata tattatctat aactcctaga gtattggaga
     3061 aaattcaaaa tatatcacaa agtaataaga atcaagataa aaacaatatg taagaataaa
     3121 atatttttaa atatgtatat agttttaagt aaagtagtgg ggttaatata aatcccacta
     3181 ttttgttgtg tactttaaac atttatatct aataagattt tagtttaaaa caatttaaat
     3241 agataataaa gttgatatat agtataatta taaaaagaaa agaagtatta tattatagat
     3301 atataaacgc atttgagata taagagggag ttataaatca ttagttttga ggtgaatgtt
     3361 atgagcgata aagaattatc tattttgttg ttagaaagta tgaataattt tcataattta
     3421 ataaagatta ttaataacga aagatataaa aaagataagg ttcttacaga aagacagttc
     3481 tttgccttag taaaaataag aaaacatgat aaaatagagc ttaaaaatct aagtagagat
     3541 ttgcatgtat ctacttctag tctgtgtata ctacttaata aattggttga gcaagagtat
     3601 gtatataggg aagaagatag tagagataga cgaaatactt tttatggaat aactaaaaat
     3661 ggagaaaaga tattggataa tgaaatacta aagtttgtat ctataataag tgataaaatg
     3721 gattgcctag atataaataa taaagataaa ttatttactt ctttggaaga gtctaaaaat
     3781 ataatagaac aattattttg aaaaggagaa tttattatga atataaagta ttctaaagaa
     3841 atattaaaaa gtttagaaaa tgctataaca gtagtagctc tatttgaggg agaagatgtt
     3901 tctacgacat ttgataaaat aaatatgata agcagaaatt ttaattctaa agaaaatagt
     3961 atagatactg taacattggt tgaaaataac aatttggtaa atgtttgtct tgttggtttt
     4021 ggtaagagag agttattgaa tagagaaaaa attagaatca taggtgggaa tttgtgtaaa
     4081 aaattaaaaa atatattgaa aaatgaagca tataagagta gtgatgttaa tgtactgaat
     4141 cttaatttaa atagtgatga aataggttgt tttgtagaag ggatattact tggaaattat
     4201 aagtttgata aatataagac aaaaagtaag gattttgaaa gaaaagaagt tgatactata
     4261 actatattta ctgaaaaaga agttgagtgt caaatagaaa aggctaaaat attagctaat
     4321 tcaactataa tagctagaaa tctagttaat gaaccatcaa acgtaatata tccaaaaaca
     4381 ttagcagtag aaacagttaa acttggtgca gagtttgggt ttaatgttga tgtttatgaa
     4441 gaagaaaaaa taaaggcatt gggaatggat gcttttttcg ctgtttcaag aggttcaatt
     4501 aataaaccaa gattcatagt aatgagatac tttggagata aagaaagtac agatatatta
     4561 ggtctagtag gtaaaggatt gacttatgat actggtggat attctttaaa atcaaatgct
     4621 tctatgctag atatgaagac agatatggct ggtgctgcga gtgttatagg tgctatgtgt
     4681 gctatatctc aaagtaaatt aaagaaaaat gttatagcag tagtggcagc atgtgagaat
     4741 gctttatcag gaggttcata caaacctgga gatataatta gttctatggc aaagaaaact
     4801 attgaagttt taaatacgga tgcagaaggt agacttacat tagctgatgc aatatactac
     4861 attataaaca atgaaaaagt aacaaaggtg gttgatgtag ctacattaac tggtgctgct
     4921 ttaatattgt taggtaatgt cgcaactcct atagtaacaa ataatgatga tttttattgt
     4981 gaattagaaa aagctgccac tttatcaggg gaaagggtat ggaagatgcc aatttatgat
     5041 gaatttaaag atatgataaa aggagaagaa gcagacctta aaaacactgg tggtaaaaat
     5101 gctggatgta taacagcagg agcttttatt ggtgaatttg ttgggaatac tccttggata
     5161 catatggata tagcggggac aagtacatca tcaaaatcaa ttggatacaa agcaaagggg
     5221 gctacaggag aacctgttag aacattgtac tatttggcag aaatctaaaa aacaattata
     5281 aaatataatt tataaagcca cgtggatatt tttgtaattt aagattatct atgtggcttt
     5341 attttttatt taaataaatt atttactctt tttctttttt tcaagtataa aagtatctct
     5401 attaaataaa agagcaggta tgtacataat actagtaaca aaaataaacg taaaaatagc
     5461 agttgtgcta tgacttaaaa aaccataata gtacctagag ttggtaaatg tgtttagtaa
     5521 aaatatgaat gaaaaataaa taaaacatat agctaagtta tctctaagtg catatctgtt
     5581 aactctaaat tttctaacat caaccatata tatatctgca taaattatca agcacttaga
     5641 gaagataaag ataaaatata ttaagctagg aaattcatag cccaagtttt tagaaaaaat
     5701 gagttctcca actgaacaaa ctaaactcca agaaaaaaat atattaaaaa gatgatactg
     5761 cttaaaaatt agtatggcta tacacagata tatacaaata taattgattc caattggtaa
     5821 agagtttaga gtactataat tacctgaaga aataagtaat aattgctcta gagccataat
     5881 tattacaagt aatgtacaaa ttatcttctc aactgtatag ctataaggta aaatattttt
     5941 ggttagtttt ggacaatagt ataaaaataa tccaaatata aagagactta caaattgttc
     6001 actagaaaaa agaaaaattt taaacatata acattctctc ctataataaa tttaatatgt
     6061 atctatataa atatattcat tatatctcaa tttaatatat aaaaaagata ataatagtta
     6121 gattttttat atgaatttta ataggtaaaa tatcctattg ttttgtagat atatcctaat
     6181 tatgtcaaaa aataaaaaaa tttttattta ttagagtttt tggaaataat aataacagat
     6241 attataatta attaacagtt tttgtaagta tttgacaata gagagggtgt aacaaatggg
     6301 aataatagag tcagcatcaa aattggctga gatggttcat ttattggctg ttgaaaaggg
     6361 gataactgat atagaagcat gggatgaagc tgtgaaagaa tacagcaaaa tttatgaaga
     6421 aagaagaaat gaatagaatg ataaaaaatg aaaacttgat gataaaaaat aactttattt
     6481 aaagaaattc aatttttata gaagtattaa aaataatata aataaaaaga tggtatttca
     6541 aatgtaagta aaaaatcatt ttgggatacc atccttatta tttattttaa taatatataa
     6601 gtaataaatc ttacttatcc atatttttta tgtaacctct tttttctaac ttttttatat
     6661 gctccttacg tatttcatca agattaatac catattgttc ttctagctta aacatcatag
     6721 taacacatgt ctgagctaca tccattagtt ccttagcagt tttctttata atatcaactt
     6781 catctaaatc attattttct ccacttagat ttctgaattt acctatacat tcagctaatt
     6841 caccttgttc ttcacccatt ttgataaatg tagattctag tgttgggtct aaattattaa
     6901 gctctggtaa agatatagtt tttagtttca taaaagctcc tttcaagtta ttcgtattta
     6961 tactaaaaca tcataacaaa aataatacta aaaaaaaact ttaaattcaa cataaaaact
     7021 tacatatata ataaaaatcg tgaaataaat ctatatttgt cttaatacag cctcatataa
     7081 aacttctcca ataggtttat ttattacaag agaagctgac ttatcataga ctgtatttgc
     7141 tttattaata agagcaatat aatcaccttt gtaataatct ataaaacttg ctgctgggta
     7201 cacagctaat gatgtacctc ctataatcaa taaatcagca ttacttatag cagaaattgt
     7261 tttagtaatg acatcactgt caagagcttc ctcataaagt actacatctg gttttactat
     7321 agaaccacag ttgtcacagt aaggaatatt acctcccaga tttaacatag attctaagtc
     7381 aaaaaactta ccacatttag tacaataatt tctatgtaca gaaccatgaa gttctagcac
     7441 atttttactt ccagccattt gatgaagtcc atcaatattt tgagttatga cagctttaag
     7501 cttacccatt tcttcgagtt ttgctaaagc tatatgagca ttattgggtt ttgcattagg
     7561 atatattaat ttatccttat aaaattcaaa aaattcttct ggatatctaa caaaaaaagt
     7621 atgtgaaact aattgttcag cagtaaattg tttattgagt ttttggctaa ataatccagt
     7681 agaacttcta aagtctggaa tattagattc agttgaaaca cctgctccac caaaaaatac
     7741 aatattattg tgattagcta ttaagtcttt taagctattt gcatccatag ttacacctcc
     7801 aaattattat atataaattg tactacatat taggtcgttt atgttttatt aatttaaata
     7861 aatatatagt ttaataagtt aaataaaggg aatataaata aaatgtatac agagtataaa
     7921 tagacaatat ttactaaaat aacaagttgt cgtataatta tattaaaatt gttttagtgt
     7981 ataagaaggg cgggggaagt acgtgaagaa aaaagcagct ttagcaacat tggctatgtt
     8041 acccttaggt gtggtaaatg cacatgctga tggagatata ggtatagtga ctataaatta
     8101 tttaaatgta agaaatgaac caactgccga aagtagcata gcctttgttg ctaaaaaaga
     8161 tgataaagtt ttgattaaag actcttctaa tggatggtat aaaataaaag ctgaatctgg
     8221 acaagaaggt tgggcttcat caaaatatat agcaaaatca aatagtgact ctttaagaac
     8281 gtctacaaat aaagaaaaac aagttatttc aaatagttta aatatgagaa atggagcagg
     8341 tacaagttac agagtaataa ctgtactaaa aaaaggtcaa aaagtagaag taatatcaga
     8401 gagtaatgga tggtctaaaa tcaaatatga tggaagacta ggatatgtat ctagctctta
     8461 tttaggagac gtttcaaact caactaataa atcaaaaact aaacaagtga atacaacttc
     8521 actaaatgta aggagtggac caaatacaag ttatggtttg ttaggcaagt tgccaaaagg
     8581 aagtaaagta gaagtaatat cagaaggtaa tggatggtcc aaaataaagt ataatggaaa
     8641 agatgcctat gtatctagta tgtacttatc ggatgtaagt caaagtaatt cagacaattc
     8701 tagtcaaagc aatgataaaa agaatactga taaggtcgta aatacagctt ctttaaatgt
     8761 aagaagtgga ccaggttcta catatagtaa gttgggaaaa gtttataaag gaagtaaagt
     8821 aactgtacta tcagaaagta gtggatgggc taagattaat tttaacaata aagaagcatt
     8881 tgtagtaggc aattatttat ctacttcagc agatacttca aataataact caaatagtaa
     8941 ctctgataac agttctaata gtaatggcaa taattcttca tcatcaggtc aagtaaatgg
     9001 tatgtcaggt ataagtgggg ctaaaattga ttataaatct ttgagttata ctttagaatc
     9061 tcatataagt aaacaagttg agaaagcagc atcaggagga aatgtgatag ctccaagtaa
     9121 taggaaaagc actccaagtc ctgaatttag tactttttca gctcaaagaa caagctcatt
     9181 tgtaaatgca agttcaagtg atatagaata ttatttaaat cctaaaaatt ttacaaatac
     9241 tactaaaggt atgatgcagt ttttaaagat taacagttat agagatggta tttcagaatc
     9301 aagtcttaac tcgtatctta atggtttatc ttctagtgta tttaaaaacc agggagcagc
     9361 ctttataaat gctgccaaga agtataatat agatgttgta tatctagtat ctcatgctat
     9421 gtgggaaact gcttatggta agtctacact tgcccaaggt caaacattaa catcttataa
     9481 gggacaacct cttagtaagc cagttaaagt atacaatttt tttggtatag gagcaataga
     9541 taaaagtgct aatgtttcag gtgcagaagc agcgtattcc aatggctgga ctagtgtaga
     9601 agctacaata gatggctctg caaagtggat atcacaaaat tacgtaaata gttctaagta
     9661 caatcagaat actatctaca agatgaagtg gaattatgat tatacatggc atcagtatgc
     9721 aactgatgtg aactgggcta atggaatttc tggaattatg gaaaatttaa ttggtcttta
     9781 tggaggagga agtagtttgg tttttgaagt tcctcaatac aagtaataca taaataaaaa
     9841 atatagacta tgggtagttt tcattttatt taaattatct atagtctttt tgtatataat
     9901 ttataatctg taaatttaat gctaacaaaa cttttatgtt ataatattaa ttctaagtaa
     9961 atataagtga gtaataggta gaaatagata aaaagtggta ataatatata taaagataat
    10021 tttgagaaag gaggatatat atggaaagta ttaaggaata tttggataag ttagaaatta
    10081 ataatagtgg tcttggaaag caacttaaag aagtttatat aaatagagta gtttatttta
    10141 aggaagataa gatagtttat ttttatctaa cttctaaaga tattgtttct catgaacttt
    10201 tagataaatt taaagaagaa ttgatgtata aactcgatta ttttaaagat atgaggatga
    10261 agataagatt tactggtctt gaaagaaaat ccaataaaga tgttataaaa aaatattgga
    10321 ataatatatt gtatatatta aagtatcttt gtccatctat tgcagggtgg tataaacaag
    10381 tagaatttct ttgtttagaa gaagaattaa aaataaaact tccaaaaggt atattttatg
    10441 agcgattaat gaaaaaaaat gtaacttatg tacttaaaac cgtgcttagc gaagagttgg
    10501 gattagattt aaatataaca atagaaaaag ctgttgatga aaaggtaaat aaagaaagac
    10561 tcataagaat caatgataga gaaatggaag aaaaaattag ggctttagaa attggaaaag
    10621 ttaataactg tgaagaaaat gaagaggaaa gttatgtcat taagtctgat gtagatgaaa
    10681 atttaatcta tggagataat gcaaatgcta tgattgaaaa tatagttgaa ttaaatgcat
    10741 cttctggaac agttgcagtt gttggagata tatttgatgt tgacactaaa gaacttaaaa
    10801 acggaaaaat acttatgata gcttcaatta cagattatac tagctcaata agctgtaaac
    10861 tttttttaac agatacaaat aaggatggag tacttgaaaa tgttaaaaaa ggagcatact
    10921 taaaaatcaa gggtgacata gtttatgata catatcaaag agaaattagt atgatgatta
    10981 gtgggataag aaaagaaact agaatagaga gagaagataa atcagaggaa aaaagagtag
    11041 aactccatgc acatacacaa atgtcttcta tggatgctat ttgttctgtt aaaaagcttg
    11101 tagagcgtgc agccaaatgg ggtcattctg ctattgctat aacagaccat ggtgttgtac
    11161 aaggatttcc agatgctatg aatgcaggta agtcaaataa tataaaaata ctttatggag
    11221 tagaagggta cttggttgaa gatgattctc tcattataga ggatgcaaat gataaagaac
    11281 tttcgcagac atttgttgta tttgacatag agacaactgg tttttctaat acaaatgata
    11341 aaataactga gattggtgct gtaaaaatag aaaactttaa gattgtagat agatttagtg
    11401 agttaataaa tcctgaaaaa gatatttctt ataagataca ggaattgaca ggaataacta
    11461 atgaattaat taaagacaag ccaaccattg aagaagttct tcctaagttt atggaatttg
    11521 ttggagatag tgtattagta gcacataatg cagagtttga tactggattt atatcgcaaa
    11581 aatgtaggga acaaggcttg ttatataata acaaaaaagt agatacactt atgttagcaa
    11641 gagtaatgtt gccaaactta aaaagacata gattaaatgt cgttgcaaaa gaacttggta
    11701 ttcctctttt aaatcaccat agagcagtgg atgatgctga agctacagca cttatattta
    11761 ataagttttt gcagatgctt acagaaaaag gtgcaaaaac tctaagtgat gtaaacaata
    11821 tacttggaaa gattgattac acaaagctta gcacaaatca tataaccttg atagcaaaaa
    11881 attctatagg tataaaaaat ttatataaaa taatttcaga tgcacatgta aatcattttt
    11941 ttagagcacc aagaatcctt agaagtgtat taaatgaata taaagaagga ttaataattg
    12001 gttcggcttg tgaagctggg gtggtttttc aagctgtaaa gaaaaacgtg tctgatgagg
    12061 aaatgaaaaa aataatagat ttatacgatt atattgaagt aatgcctata gataataata
    12121 gatttatgat tgataaagga gaagtaaagg acgaagaaga gttaagggag ttgaatagga
    12181 aattaattga tacagccaaa aaatttgaca aaattccagt agctactggg gatgttcatt
    12241 ttttagacaa acatgaagct gtcttaagaa aagttcttaa atattctcaa ggttttaaag
    12301 tagatgaaga agaaacttat ttacatttta gaacaacaga tgaaatgttg gaagagttta
    12361 gttatcttgg agaagaattg gcatatgaag ttgtagttga aaatagtaat ctgattgccg
    12421 atatggttga agatataaaa cctataccag atgatactta tcctccaatt atagagggct
    12481 cagatgtgga gttaagagaa atgtgttaca aaaaagccaa acgaatctat ggagacccaa
    12541 taccagaaat agttcaaaaa agactggata gagaattaaa ttcaataata agcaatgggt
    12601 atgcagttat gtatattata gctcaaaaac tggttgcaaa atctctttca gatggatatt
    12661 tagttggttc aagaggctca gttggctcat cttttgcagc tactatgagt gatataacag
    12721 aagttaatcc tcttccagca cattatattt gtgaaaacga agactgtaaa tattcattct
    12781 tttatgaaat aggtgaatgg ggctcaggtg ttgatttacc agacaaggat tgtccaaagt
    12841 gtggaagaaa acttgctaaa aacggtcatg atattccatt tgaggttttc ttaggttttg
    12901 aaggtgataa agaaccagat atagacttaa atttctcagg aaattatcag ccaacaattc
    12961 ataaatacac agaagaacta tttggagagg ggtatgtata tagggctggg actataggta
    13021 ctgtagcaga aaaaacagct tttggatacg caagaaaata tgtggaagaa aataatataa
    13081 gtgtaccaaa tgcagaagta cttaggcttt caaatggatg tactggagta aaaagaacct
    13141 caggacaaca tcctggtgga gttatggtta taccagacta taaagatgta tacgatttta
    13201 cacctataca gtatccagca aatgatactt cttgtggtgt tataactact cactttgatt
    13261 atcattcaat aagtggtaga atacttaaac ttgatatact tggacacgac ggtcctacca
    13321 ttataaggat gcttgaagat atgactggaa taaatataac agaaattcct ttagatgata
    13381 aagagactat gtctctattt acatctacag aagcattagg tgttacacca gaagaaataa
    13441 actgtcctat aggttgcctt gctgtaccag agtttggaac taagtttgta cgacaaatgt
    13501 tacttgacac aaaacctact acatttgcag aacttgtacg tatatctggt ctctctcatg
    13561 ggacagatgt ttgggtaaat aatgcacaag acttagttag agaagatata gttggattaa
    13621 aagatgttat atctacacga gatgacataa tgaactattt aatatttaaa ggattacctc
    13681 ccaaaatgag ctttacaata atggagagtg taagaaaagg taaaggttta aaaccagaac
    13741 acattgaaga aatgaaaaaa aataatgttc cagaatggta tataggctca tgtaaaaaga
    13801 ttaagtacat gttcccaaaa gcacatgctg ttgcttatgt aatgacatca tttagaattg
    13861 catattgtaa agttcactat ccagaagcat tttatgcgac ttattttacg acaaaagttg
    13921 aggattttga tgcagattta attgttaaag gattagatgc tataaaatca aaaataaatg
    13981 aaattgagtc actgggaaat gatgcaacca caaaagaaaa aggtatgttg accgttttag
    14041 aagttgctct tgaaatgtat gctagaggaa taaaattatt accagttgat atatataaat
    14101 cagatgcaac tgagtttata gttgtaggag aaaaaacatt acttccacca atggcagcaa
    14161 tacaaggtct tggaggaaat gcggctataa acatccaaaa tgaaagaaaa aatggtgaat
    14221 ttatatcaaa agaagatttg agaaagagaa caaaaatatc aaaaacggta atagaaactc
    14281 ttactaatca tggttcactt gagaatatga gtgagagaaa tcaattgtca ttgttttaag
    14341 ttagcccaaa aagttgcaaa taaaagaatt ttatgatatc atagtataga aaatagtata
    14401 ctttgcagaa actaagagtg agacagctgt tctcactctt ttattttaaa tatattatta
    14461 aaaagctggt ttatttttat taaatatatt ttatttaaga ttatataaac tggtcattta
    14521 caattgaata ggaggaactt taatgaaaaa aaatttagaa gctacaattg aggagattgt
    14581 aactaaaata acggatgaac atggatttga aatggttgat gttgaatatg taaaagaagc
    14641 aggagaatac tacttaagag tatacataga taaagaagaa ggaatatctt taaatgaatg
    14701 tgaattagtt agtagagaat taagtccaat actagatgaa aaagacccta taaaagaaaa
    14761 ttacttttta gaggtatctt ctccagggct tgatagagca ttaaaaaaag acagagattt
    14821 tgttagatat caaggtagag atgtagattt aaaattatat aaaccattaa atggatgtaa
    14881 gcaatttgaa ggtgaacttg taggattaac agaagataat aatataaaaa tcattgcaaa
    14941 tggtaaggaa atggaattta atagaaaaga tgttgctatt gtcagattag caataaagtt
    15001 ttagtggtat attaaatagg gaggatataa aaaatgaatc atgaatttat ggaagcatta
    15061 gatgaattag tagttgacag aggtatagat aaagaaatac ttatagatac tatagaacaa
    15121 gctcttttaa ctgcatacaa gaaaaacttt ggttcagcac aaaatgttag agttgaattt
    15181 gatagagaca agggagatat aaaagtattt tctcaaagag ttgttgttga tgaatcagat
    15241 ttatatgata ctttcttaga aatagagtta gcagatgcta gagagataaa ccctaattat
    15301 gaattaggtg atattataga acatgaagtt acacctaaaa actttggaag aattgcagct
    15361 caaacagcaa agcaagtagt tgttcaaaga ataagggaag ctgagagaga aatagtgtat
    15421 aatgaattta tagaaaaaga aaatgaaata gttacaggag aggttgctag agcaaataaa
    15481 aatgttgttt atgtaaatct tggaagaata gaaggtataa tgactcaaag tgaacaaata
    15541 ccatttgaga attataaagc aggtcaaaaa ataaaattct atatacttga agttaaaaag
    15601 actaacaaag gacctcaaat agttgtatca agaagtcatc cgggtctagt aaagagattg
    15661 tttgaagaag aagttcctga aatatatgaa ggaatagttc aaataaaatc tgtagcaaga
    15721 gaagcagggt caagaactaa aatggcagta agatctatag atgaaaaaat agacccaatt
    15781 ggagcttgtg ttggaccaaa aggacttaga gtaaaaaata tagtggatga acttggcgat
    15841 gagaaaatag atataataaa atatagtgaa gacccagcag agtttatatc agcagcactt
    15901 agcccatcaa aggttgttag agtagatata gatgaagatg aaaagtctgc cttagtaatc
    15961 gttccagatt atcagttatc gcttgctata ggtaaagaag gtcaaaatgc aagactagca
    16021 gcaaaattga ctaactggaa aatagatatt aagagcgaat ctcaagcaga acaagaaatg
    16081 ttaaatcttg aaaattccaa agaaatagaa gctgaggatg ttgaagctga atttatcgac
    16141 caagaagagt tagatacttc agaggataat tttagtgaag aagagtaagg agggatgatt
    16201 ttgaaaaaag taaaaaaagt gcctcaaaga aaatgcatag catgtcaaga tagagactct
    16261 aaaaaagagc ttataagaat agttaaaaat aaagaaggaa aaatattttt agacccaaca
    16321 ggaagagcca atggaagagg agcatatgta tgtaaaagct ctgaatgtct aaaaaaagct
    16381 ataaagagta aagcattaaa taaagctttt aaaatagatg ttcctgatga agtctatgat
    16441 aatctactta tggagttaga agaatatgaa aaataataaa gaaaaagtac tttcgttctt
    16501 agggctcgca actcgtgcag ggaaaatagt gtctggtgat gattcaactt tgcttgacct
    16561 taaaaaagga aaagtaaatt tgattttaat cgcagaagat gcatcaaata atactaaaaa
    16621 actttttaaa gataaatcaa cttttagaaa tattccatat ctattttttt caacaaaaga
    16681 ggaaatagga tttgcaatag gaaaatctcc aagagcagtt gtaggaataa aagatgaaaa
    16741 tttttctaaa aagataattg aactaataga aatttaaaat aaaatagggg gtgatcttgt
    16801 gtcaaaaaca agagtatacc aaatagcaga agaattaaat atatcaaatg aagagcttat
    16861 aaataagtta gctgaattag atataaatgt aactgacaaa gattcagtat tagaaggtga
    16921 agagttagaa ttagcacttg aaatgttagg tgaagattta tctcaagaaa atggaaatgt
    16981 tatagaaata gatggaaagt tgacagtaca agtattagca acgaaattag ataaatctcc
    17041 atcagaaata attatgaagc ttatgaaaat gggaactatg gcaacaataa atcaagagat
    17101 aagttttgag attgctgctt tggctgccaa agactatgga tttgaattaa cagtagctga
    17161 aagtgatgat acagaagctt tagaaataga agctttaatg gaaattgaag aagataaaga
    17221 agaggattta aaacctagac caccagttgt tacagttatg ggacatgttg accatggtaa
    17281 gacatcttta ctagatgcaa taagaaaaac tgatgtgatt tcaggtgaag ctggaggaat
    17341 aactcagcat ataggtgctt cagaagttaa aatcaatgga cacaaaatag tatttttaga
    17401 tactccagga catgaggctt tcacatcaat gagagcaaga ggagcacaag ttacagatat
    17461 agcaatatta gtagttgcag cagatgatgg tataatgcct caaacagttg aagctataaa
    17521 ccatgctaaa gcagcaggtg tacctttaat agttgctatt aataaaatag ataaaccagg
    17581 tgcaaaccca gataaggtta aacaggaatt agcagaccaa ggattattag ttgaagactg
    17641 gggtggagaa gtaatagcag ttccagtatc agctaagaaa aaagaaggaa tagatacact
    17701 tttagaaatg gtattattgg ttgctgaaat ggaagagtta agagctaatc caaataaaag
    17761 agcagtagga actgttatag aagctgagct tgataaagga agagggccag ttgcaacagt
    17821 acttgttcaa ggtggtactt taacagtagg tgacccaatc gttgcaggtg ttgcatgtgg
    17881 taaagttaga gcaatgataa atgcaaaagg taagagagta aaaacagcag gaccatctac
    17941 agcagtcgag atattaggtc tatcagaagt tccacaagga ggagaccaat ttgtcgaagt
    18001 tccaactgat aagattgcta gaagtgtagc agcaagaaga caacaaatag ttagagatga
    18061 aatgttaaaa tcaactcaaa gattatcact agatgctcta tttagtcaaa tgagtgaagg
    18121 ttctataaaa gaccttaata tagttataaa agcagatgta caaggttcag tacaggcagt
    18181 taaacaatct ttagaaaaat tatcaaatga agaagttcaa gttaaagtaa tacatggtgg
    18241 tgttggagct gtaacagaat cagacatatt acttgcagca gcttctaacg caataataat
    18301 aggattcaat gtaagaccag ttccaggtgc agaatctcta ggagaaaaag aaaatgttga
    18361 tataagaaca tatactataa tatacaaggc aatagaagat atacaagcgg ctatgactgg
    18421 tatgttagac cctgaatatg ttgatgaaga aacaggaaaa gctgaaataa gagaaatata
    18481 taaaatatct ggtgttggaa cagtagctgg atgttatgtg actaatggta agatatttag
    18541 aaattgtaaa gtaagattag ttagagatag cataataata catgaaggtg agcttgcagc
    18601 acttaagaga tttaaagatg atgtaaaaga agttaattct ggatacgaat gtggtatgag
    18661 ttttgtaaat tataacgaca taaaagaagg cgatatagta gaagcttata taactaaaga
    18721 agtagaaaga aaattataaa ttcgtatcaa agaaaatcag ttagtaaaga aggtcgatgg
    18781 tatggcatca tataatagaa caagaaggat agcagaagaa ataagaaaag tcgtaagcac
    18841 tatgcttata aatggagtaa aagaccctag aataacatct atggtgagtg taactgatgt
    18901 agaagttaca aatgacctaa gatatgctta tgtatatgta agcattctag gtggagatga
    18961 agaatccaca ctaacaggtc ttaaaagtgc tggaggatat ataagaagag aagtaggaaa
    19021 aaatataaaa cttagatata ttcctgaaat agtatttaaa cttgatgatt caatagaaaa
    19081 aggaatgtat atggatagcc ttataaaaag agttaatgaa aaaaatgctc aacaaaatga
    19141 ggatgaaaat tatgatgaat aatatggata gtattataaa ctatatttat gagggtgact
    19201 attttatagt tacctctcat atcagtccag atggagacaa tattggttct acactttcaa
    19261 tgtattatac attgaagaat ttaggaaaaa atgtatatta tgtacttgat gatgaagctc
    19321 cattaaattt aaggtttttg gttgaagatg taaaaatcta taagtctaat gaatttaata
    19381 tgaagaacta ttctctaatt gcacttgatt gtggggataa gatgagaata tgtgtgagtg
    19441 atgaaatcaa aaataatgca tcaaaattaa tctgtattga ccatcatgct agtaatgatt
    19501 catatggaga tttaaattat gtagatattg atgcatcttc tacatgtgaa ttagtttata
    19561 atttattagt tagatttcag caaataaaag atattaatat tataaatgaa gatatagcaa
    19621 catgtttata tacaggtctt gtaactgata ctggaaactt ttcgtattca aatgtacatg
    19681 caagtagctt tgaaatggca aaaaatttac ttgttttagg tgcacaaaaa aatactataa
    19741 tacaaaatat atatcaaagc aattcttctg gttattacaa attattagga gaagctctaa
    19801 agggtttgga gatatttgat tcaaaggtat ctagtatagt tttaactcaa gacatgatga
    19861 atagaaataa tattagcttt aatgatgttg atggaataac aacatataca agagacataa
    19921 aaggtataga agttggaatt ttatttaaag agaaaaaaca aaatgaaatt aaagtgagtt
    19981 ttaggtcaaa aaattatgtt gatgtaagtg aaatagccaa gttatttggt ggtggtggtc
    20041 atgtaagggc tgctggatgt actattagag atagtattga taatgcaaag aaaatggtac
    20101 tagaggcagt gttaaaatca atttaaggtg acagtaaaat gaataagatt ataagtatat
    20161 tgaaacccac aggtatgaca tctcatgatg ttgtaagtag agtgagaaag attttaaata
    20221 taaaaaaagt tggtcataca ggtactcttg acccagatgc ttcaggtgtt ttaccaatct
    20281 gtatagggaa agctacaaaa gtatgtgaag ttattttaaa caaagataaa tcgtatatat
    20341 gtgagcttac tttaggtata agtacagata cttatgatgc ttcaggtgaa attttaaaaa
    20401 aagttgatga ttttaaattt agtaatgaag acatagaaag agcttttgac acacaaagag
    20461 gagagataaa tcaacttcct ccaatttatt cagctttaaa ggtaaatgga aagagaatgt
    20521 gtgatttagt aagaagtggt agacaatctg aaattacttt aaaaacaaga agagtaaata
    20581 taaaagacat aaaaatacta agtataaaag gtaataaagt aatgttttat gttgagtgtt
    20641 ctaaagggac ttatgtgaga agtatttgcc atgatatagg agaatatcta ggttgtggag
    20701 ctcatatgtc gtttttgaat agaacatctt ctggtaaatt tgacttagat aattcaataa
    20761 cattagaaga attagaatta ttttatgaaa ataaaacttt agacaaatac ttatatgata
    20821 tagattatgt ccttgattcg tttaattatg ttgtattaaa ccctaatgct ataaaatatt
    20881 atagtaatgg tggctcaata gatgataaaa ggtttttaaa gaataatttc gataaagatg
    20941 atgaatttgt aagagtatac agtacagata atttcttagg attaggaaag ttatcaaaac
    21001 acaataatac aataagtgtt aaaagtgata agatgttcat ataaccaatg tatttaaaaa
    21061 aacaacaaaa gagatagtac tataataact tctatagtct atctctttta cttatgctat
    21121 aatagtttgt gaataagtta agagtaaatt atattttagc ggggaacatt tattatggtg
    21181 attataaaat ctataaaaga aatagcaaac ataaaagaaa gtgtcattac cataggaaat
    21241 tttgatggtg tacacaaagg acatcaagtt cttataggaa aaacagttga acatgcaaaa
    21301 aaagaaaata tacagagtat tgtatttaca tttgcaaatc atcctgtaaa ttactttaga
    21361 ccaaactcaa caaaaaatat tataagcaat gatgataaaa taaaattatt agaagatttg
    21421 ggaatagata tagttgtaat cataccattt gatgagtata tgactaaaat acctgcaaag
    21481 gatttcgtag atgagatact agtaaaaaaa ttaaaagcta aaaagatagt aattggacat
    21541 gactttacat ttgcaagggc aaaagaaggc aatgttaatc tattaaaaag cttagaacat
    21601 gaatttggtt ttgaggtaga agtaataaaa cccatcaaaa tcaatgatgt aagagtgagt
    21661 agtacttata ttagaagttt agtatcccag ggtgatatgg ctaatgtcaa ggaatatttg
    21721 ggtagaaatt acaagttaga aggttgtgtt atacattcta aacaccttgg aagaactata
    21781 ggattcccaa cggcaaatat tgaccttaag aataatatgc tcgttccaaa gagaggtatt
    21841 tatgcttcta ttgtacatat tggagatgag acttattttg gtgctacaaa tgtaggctat
    21901 aacccaacag ttaatggaaa aactttgtct attgaaacaa atattttaga gtttgataga
    21961 gatatttatg gggaaaatat aattgttgaa tttttggaaa gaataagaga tgaaagaaag
    22021 tttaattcaa tagatgactt aaaaaatcaa ttgtataatg atacaaatta tgtgtatgaa
    22081 aactatgttt gcaaaaacaa gtaatgtatg ataaaatgat taaggaaaat aaccattact
    22141 cggcatttga atactccaat caatgcttag taataggcgt ttctaaaaaa atacggaggt
    22201 aatctaaatg ataaacaaat cagaaataat aaaagaatat ggaagagctg aaggggatac
    22261 tggttctcca gaagtacaaa tagctttatt aacagctaga ataaacgagt taaatggaca
    22321 tttaaaaact cataagaaag accaccattc aagaagagga ctattaaaaa tggttggtag
    22381 aagaagaaac ttattagctt acttaaagga aaaagatctt gaaggatata gagcattaat
    22441 agctaaatta ggattaagaa agtaatttca ttctttataa aaaagcaggt atagtgtata
    22501 cctgcttttt tatagttaat tcattataaa gtaaaatagt taataataaa agtagtttat
    22561 atgttataaa aaattataaa atgaataata ttgggtaaga atattaaaat agtatacaaa
    22621 tacataatca atttaaaagg aaagacagga taaatataga aattttataa caggataatt
    22681 agttggaata aggaggatat tattttgaaa ataaagttag tatgcgatag tttgtgcgat
    22741 attccagatg aaatatcgga aaaagattat atagagatag ttccacttac agttattttt
    22801 aatgataggg aatatataga aggagtagat ataaagaaag aagagtttta taaaaaagta
    22861 aaagaaatca agcaaatacc taaaacttca caagctacat atatggagtt taaagaagtg
    22921 tttgataagt tcataacaga aggttatcat ataatctgta tgacaggggc tgcaaacgca
    22981 tcaggaactt ttcaaagtgc tatgatagct aaaaatgatg taaatgaaaa tgaaaaaata
    23041 catatatttg atactagaaa tttatctcta ggtagtggac aatatgttat aaaggcatgt
    23101 gaacttcttg aagaaggact gggatttgaa gaaattatag atgaattaga aaatactcgc
    23161 agtagtgtaa gattgctttt tgcaccatat acacttgatt ttttaaagca gagtggaaga
    23221 gttccagttg caactgcttt gataggaaat atgttaaata taaagcctat atttttcttt
    23281 gataatgggg aagctaagtt ggtaaataag gttagaggaa taaagaatat agcaagtaaa
    23341 ttggtcgata taattttaga aatgaatgaa gggtctttag aaggaaaaat cgtcacaata
    23401 ggttgtggag ataatttaca cgattgtgaa atattaaaag atgaagtaaa taaaagaata
    23461 aaagcaagaa gagtattatt tactcgagga ggagtttcta tttgctctca tacaggacca
    23521 gatatattgg ctataagttg ttctaattag tagtaatata aggtttttgc atatatatgt
    23581 gaagataatt ttatattgtt aatttatagt atttttgata atatagtaaa tagagtgata
    23641 tacgtgtttt ttaataagag gaggacaagc attaatgttt gaacataaaa tatttaaaat
    23701 ggattttgca ggaagagaac tttctgtaga aataggaaag atttgtgaaa tggctagtgg
    23761 tagttgtata gttcgataca gtgatagtat ggtaatggta aatacaacta aatcagccaa
    23821 acctagagac ggaatagact tttttccttt aagtgtggat tatgaagaaa aattatactc
    23881 tgtaggtaag atacctggag gttttttaaa gagagaaggc aagccttcag agaaagctat
    23941 acttacatca agattgatag atagaccaat aagaccttta ttcccaaaag gatttagaaa
    24001 tgatgttcag gtagttgcaa cagttctatc agtagaccaa gattgtacac cagatatagt
    24061 agcaatgata ggttcttcaa tagccctttc tatatcagac ataccattta atggaccaac
    24121 aggttcagta tgtgttggcc ttgtagatgg tgcatttgtt gtaaatccaa acgcagagca
    24181 aagagaaaaa agttcaatgc atctagttgt ttcaggtaca aaggaagcaa taatgatggt
    24241 tgaagccgga gctgatgaag ttccagatga ggtaatgctt gatgctattt tatttgctca
    24301 ccaagaaata aaaaaaatag ttgagtttat agaaggtata gttgcagaag ttggaaaaga
    24361 aaaaatgcct gttgaattat accatgcagg agaagaaata actcaattgg ttcgcgaatt
    24421 tgcaactgat aagatgaaaa aagcagttca gacttttgaa aagttagaga gaatggaaaa
    24481 tatggataga gttaaagaag aaactttagc tcactttgaa gaaactttag aagattttga
    24541 agattttgtt ggagatatag aagaagtatt acaagatata ataaaagaag aagtaagaaa
    24601 acttatagta catgaaaatg taagaccaga taatagaaaa cttgaagaaa taagacctat
    24661 atggtgtgaa actggcatga tacctagagc tcatggttca gccatattta ctagaggaca
    24721 aactcaagta cttaatgttg ctactttagg tgctttagga gatgttcaaa aactagatgg
    24781 gttagatgaa gaagaaaaca agagatatat gcatcattat aatttccctg catatagtgt
    24841 tggagaagct agaccatcaa gaggtccagg aagaagagaa ataggacatg gtgctttagc
    24901 agaaagagct ttattaccag taataccatc tcaagaagaa tttccttatg ctattagatt
    24961 agtttctgaa gtattaagtt caaatggttc tacatctcaa gcaagtgtat gtggttcaac
    25021 attatcttta cttgatgcag gtgtacctat aaaagatatg gttgcaggta tagcaatggg
    25081 acttataaag catgatggta aggttgcagt gctttcagat atacaaggta tggaagacca
    25141 tttaggcgat atggacttta aagtagctgg aacagagtat ggtataacag caatacaaat
    25201 ggatataaaa attgatggta tagataaaga aattctacaa cgtgctttaa aacaagctaa
    25261 agagggtaga atccatatac taggagagat gagaaaaact atatctcagc caaaaccaga
    25321 actttcacca tatgcaccta agattgttaa aatgcaaata aatcctgaca agataaaaga
    25381 tgtaatagga cctggtggaa agataataac taagataata gacgaaactg gagttaagat
    25441 agatattgaa caaactgggg aagtatttat atcaggtatt gagattgata tgattaagaa
    25501 agctcaagaa cttataaata atatagtagt tgaaccagaa gtaggaaaaa cttataaagg
    25561 taaggtaagt agaataatga actttggagc atttgttgag atacttccag gaaaagaagg
    25621 attacttcat atatctcata tagctcatga aagagttgcc aaagttgaag atgtattgaa
    25681 tataggggat gaagttgaag ttaaagttac agagattgat gaaaaaggaa gagttaattt
    25741 atcaagaaaa gttcttttac caaaaccaga acataaaaat aaataactta ttatataatt
    25801 aaaagcaaag tcttaggatt ttgctttttt aaatacaaaa aagtagataa ttttaaaatg
    25861 atagttatgg atggcttata gttctttttc tgtcagtctt tactcttata aagaagctat
    25921 ttaaaagctg attaatagaa agctttattg atatatatag aatcacttaa gttatattta
    25981 gtgcataaac agagcttatt tttaatagta ttaaataaga aacaatttgt acataattag
    26041 ggggagtttt aatgataatg atggtactta ataagagtaa gctcaaaaaa atcataatac
    26101 taattttagt agtgttatta gtattatcag gaggggtaat ggtattaaag ttatttaaca
    26161 acaagccagt atttaattta tttaatggtg tagatttacc atatgaaaga ggtacaaagg
    26221 ataaaagtgg atatgttgca ttaacctgta atatagacct tggatgggaa acagagtacg
    26281 tagaatccat tttagaaaca cttaaaaaag aaaatgtaaa gattacattt aatgtaactg
    26341 gaaaatgggc agaaaagaat aaggatgaat tattaaagat aaaaaaacaa ggacatgaaa
    26401 taggtaatca tggatataaa catttagatt attctacatt atcatatgaa gacaattatg
    26461 agcaaataga gacctctaaa aagataattg aagaaattat aggtgaaaaa actaaatttt
    26521 ttcaagcacc agcaggttcc tttggtccag aaacagtaaa ggctgctaaa gcattagggt
    26581 atacttctat caaatgggat gccgatacta tagattggaa atacaaagac caaccagagg
    26641 taatcattga tagaatgaag aaaaaagaca ttaaagatag tagtataatc ttaatgcatc
    26701 caaccaacgc aacgacaaaa tgtatagatg atattatagc tattgtaaga gaaaaaggac
    26761 taaaagcagg aaaacttagt gatgtattta agtagacaaa gtataaatat ttggttaaga
    26821 ttgtataagt tgtagttgaa ttaattaaaa gactgactta taatgtaata catataaaaa
    26881 ataaatataa ttaaaacata gtgttttcaa taatccaata tgaaacaaag aataaattgt
    26941 taggagaatg taatgtataa gacaaaaata ctggaaaatg ggcttacaat aattggtgaa
    27001 gaaataccat atctaaagtc aataacatta ggtatttgga taaatgcagg ctctaggata
    27061 gaagaagcac aggtgagtgg aacttctcat tttatagagc atatgatgtt taaaggaacc
    27121 aaaaacagaa catcaaaaga gattgctagt tcaatagata atttgggtgg tcaaataaat
    27181 gcatttacaa gtaaagaatg tacatgttat tatgtaaaac taatagatga gcatatagat
    27241 acaggaattg atgtattgag tgatatgata ctaaattcta aatttgataa aaatgatata
    27301 gataaggaaa gacttataat attggaagaa cttaagatgt atgaagactc tccagatgac
    27361 ttatcttatg atttattagt ggaaaatata tatgctaatg atggattagg aatgaatata
    27421 attgggacaa aagaatctct atacaatata actagagaat ccatgttaga atacttaaat
    27481 aagtattata ttccaaataa cgctgttatt tctatagctg gaaactttaa ctttgatgat
    27541 atggtggaga aaatcaaatc aaaatttgga cactgggaaa agaaaaattt gagtatagac
    27601 ataagtgagg ctaagtttaa tccttgcttt atatcaaaaa ataaagatac tgaacaggtg
    27661 aacttagcta tgtgcctaaa aggtattcct tttgaaaatg atgaagaagt ctattcaatg
    27721 gcagtggtaa ataatatttt cggtggaagt ataagctcaa ggctttttca gaaaataagg
    27781 gaagaaaaag gtttagtata ttcaatatac tcatcacaga cattatatag gaaatgtggt
    27841 gaattaggta tatttgcaag catgagtaca gaaaatttac aagatgtata taatttaata
    27901 aagaaagaaa ttgaaaatat aagagaaaac tatcttacag aaaaagaaat atctgaaagc
    27961 aaggaacagt taaaaggaaa ttatatattg gatttagaaa gtactagcag tagaatgatg
    28021 tctactggta aatctatgtt acttagtaaa aaagtaaaaa ctacagatga aatacttgaa
    28081 tgtataaata gtgttaatat taattcaata aagaaggttg ttgataaggt attcaatatc
    28141 gaaaatattg gtacatgtat agttggaaga gatgtagaaa agattctaca cttggattaa
    28201 tattgaaaaa agtaaatctc tggaggtata aaatatgaat ttatctgaaa tagcaggtaa
    28261 ggaaatagtt aacttggtca caggagaaag attgggggtc attggagaat gtgaccttat
    28321 actagatgaa actacaggcg gtatattagc gcttctaatt ccaaaagaaa aaggattttt
    28381 tgccagaaga aaagataagt catttttaga agtaccatgg agaaatgtaa aaaaaatagg
    28441 taatgacatg ataattatag aatatgaggg atatatttag gaggaaaaaa tgagtatttt
    28501 agtgcaaaag tttggtggga catcagtaga atcatatgaa aaaatgaatg aagtatgtaa
    28561 gatagtaaaa gcatacaaaa aaaatgatga agaattacaa ttagtattag tagtttcagc
    28621 aatgggaaga aaaggagctc catatgctac agatacactt ataaatttgt gtagtgcagt
    28681 aaatgatgag ccaagtaaaa gagaacttga tttaataatg tcttgtggag aaataatatc
    28741 aggaacaatt cttgctaatt tattgaatgc acaagggata gattctgttt tctttactgg
    28801 tcagcaagca ggtataatta caagtgatga atattctaat gcaaagataa agtatataaa
    28861 tcctaaaaag gtaaaaagag ctttagatga tggtaaggtt gtagttatag ctgggttcca
    28921 gggtgtaaca gatgatggag aaataactac tcttggaaga ggcggaagcg atacatcagc
    28981 agttgctatt ggtaaagctt tagaatgtga aacagttgaa atttacactg atgtggatgg
    29041 tataatgact gcagacccta gagtagaacc aaacgctaaa gtattgagtt ttatagacta
    29101 tgaggaagta ttccaaatgg ctgacaaagg tgcaaaagta atacatccaa gagcagtaca
    29161 gttggctaaa agtggaaata taactttagc cataaagaat acaatgaatc caacttttga
    29221 aggtacaaaa ataggttctt tatgtagaca cttagaagac aatatagaat atgaacaaga
    29281 aagagatttt aaggttgctg ttgcaaataa agatagcgta gcacaagtta agattaagtc
    29341 agcagaagaa gtgtttacag aagtattaaa tgagatagaa aagaaactta taactataga
    29401 tatgataaat ttctttatat cagaaaaagc atttgttgta gaagatgctg atataaaatc
    29461 attaaaggaa atactagata aatttgaatt agattatgaa gtaaatagag actgtgctaa
    29521 ggtaacattg atatgttcaa gaattgctga tgaaatgtct ggtatcatgt caaaggtagt
    29581 tagaggatta tcaaaagcag gggtatccct tttacaaaca tcagactcaa atatgactat
    29641 atcttgttta gttagtgaag aagatatgca tacagcagta catgctatac atcaacaatt
    29701 ttatttaaaa taaactgtat aatacaatta ttttttaatc aaatttaaga atttcatata
    29761 tatcttaata tatttgattt atttggtaaa actaaattaa atataatttt gataggagtt
    29821 tttttatgaa agattataga gaagataatg aaagatttga taaagataat tgtagtgaag
    29881 aatctagttg cgaagagaga gaatgtataa agcaatttgg taccaatgaa atgccacctc
    29941 aacctcctaa agatattcaa tgtataacta taattggaga aattgaggga cattttatag
    30001 gaaatcctca aaaaaaagct acaaagtacg agcatataat tccaatgcta tactcaattg
    30061 aggaaagtaa tgatgttaaa ggagtgttag ttgttttaaa cactgttgga ggagatattg
    30121 aagcaggttt agctattgca gaacttttaa acagtacatc taaaaaagta gttactcttg
    30181 ttctaggagg aagtcatagt ataggtgttc ctcttgctac agcaggagat tactcattta
    30241 tagcaccaac agcaaccatg attatacatc cagtgcgaac tactggtctt gtgattggga
    30301 taaatgagac ttttgagtat tttaaaaaga tgcaagatag aataattcaa tttataataa
    30361 gaacatcaaa tatcaaaaaa gatgtacttg aaaaacttat gcatgaaaaa gatgaacttg
    30421 taagtgatgt tggtagtgta ttaataggaa aagaagctgt ggattatggt cttatagatg
    30481 aagttggagg actaaaagaa gcacttaaaa aattaagaga gcttataaaa gaatctgagg
    30541 aagaaaaaaa taatgattaa aatttgttta aataataaaa tttatgatat aatgatgtaa
    30601 taaagtagga gtgttagtgt taaggcatta gcactttttg ctgtgaaggg gtaaaacttg
    30661 ctaaaaataa ggagtgatat taatggccac gaaaaagaag aaaaaaaaga aggtaaatac
    30721 cttaagtttt aatgcagaat atcataattt aataacaata tttataggtg tatttttatt
    30781 atatagttta aattctaatt ctatgggatg gatacctgtg ttaatgcaaa atctttttaa
    30841 gggactattt ggagggcttt caatagcaat accatttata gtaattataa caggactttt
    30901 aggatttttt gatggaaatg aatatatata taggctaaga aagactaaac tatactacat
    30961 aattatttta tttatatttg tattttatgg attattaaat gcaggaactc ttcctgtaga
    31021 tagcccacta aaaggtaaca tgtttgatga tgttatgaaa ttgggtgtaa gtggacaagg
    31081 tagtggtttg atagcaacaa ctattgcgta ttatatgagt aaaatatttg gtatagcagg
    31141 tggatggtta ataagtatct tcgcacttat actttctgta atgtttatat ttaatatttc
    31201 tataaaagat ttggtgtcaa atgcaaaatc aaaatctaaa gcatctaaag acagcaattt
    31261 aacgtttaaa gataaaattg caaatatgaa aaaatctgca atagatatga tgactgatga
    31321 agtagatgat actactataa ataaaaaacc aggtttcttt aaagggttaa tgtcaaaagg
    31381 tcttaataat gatgatgaag aagatgaata ttttgaagaa gaaaatagtg atggtgtaga
    31441 tgataaaact ataaaaatag ttggatttaa taaggcagaa gatgagtatt tagaaatatt
    31501 agaaggaact caaagtatgc cagagttaga tgttttaaaa gaattacaaa aagctaccaa
    31561 tgaaaatcca gttgtagata ctaaaccaga aaaaaaagtt gatatagcta aacctaattt
    31621 aaatatagaa aaaactcaac ctatgagtat tgtagcagag ccagttaatg aggattattc
    31681 aaactataaa aagcctagca ttgagctttt aaataaagta aataagaagt ctgatgaaaa
    31741 tggtaaaaag aaagtgttga aaaatgcttc tcttttagaa aagacactat cagattttgg
    31801 agtggaagca aaaattaatc aagttacagt tggacctact ataacaagat atgaaataca
    31861 accaagtcca ggagtcaagg taagtaaaat tgtaaattta actgatgata tagctttaag
    31921 tttagcagct aaaagtataa gaattgaagc cccaattcct ggtaaaagtg caattggaat
    31981 agaagttcca aatgaagaag ctcaaatggt tggtgtgaga gaagttctag aaagtgaaga
    32041 atttaataat tttaactctc ctcttgcaat gggacttgga aaagacgtag caggaaaaat
    32101 tataattgga gatataggta agatgccaca cttactaata gctggttcta caggttcagg
    32161 aaagagtgtg tgtgtaaata cattaataag tagtatttta tataaggcaa atcctgatga
    32221 agtaaaatta ctattaatag acccaaaagt agttgaactt gcaaattata atggaatacc
    32281 acatctattg ataccagtag ttactgaccc taaaaaagct gcaaatgctt taaattgggc
    32341 agttacagag atgaatagaa ggtacaaatt atttgcagat gctcaagtaa aagatgtaac
    32401 aagttataat gagaaagcgg aagaaaaact tcctaaaata gtaataataa tagatgagtt
    32461 agcagattta atgatggcaa gtgcaaatga tgtagaagat tatatttgta gattagctca
    32521 aatggcaaga gctgctggaa tgcacttgat tgtagcaact caaagaccat ctgttgacgt
    32581 tataacaggg gtaataaagg ctaatatacc atctagaata gcatttgcag tatcatctca
    32641 aactgattca agaacaatcc ttgatatggg tggtgcagag aagcttctag gtaaaggtga
    32701 tatgttattc tatccacttg gtgcagcaaa accagttaga cttcaagggg cattcatatc
    32761 agagagtgag tcggaaaaag taatcgattt tgtaaaaagt caagttaaag atggaataaa
    32821 atatgaagaa gatataattg aaactatatc taaggttaat acttcaaagg gtagtgatga
    32881 ggatgagttt ttgagtgaag cgatagaatt tgtcgtggaa agtggtcaag catctgcctc
    32941 tatgctacag agaagattta aaataggatt caatagagca gctagactta tagattctat
    33001 ggaagaaaga ggaataattg gagcaagtga gggaagtaaa ccgagaaagg tattaataag
    33061 caaacaggat ttgcagaatt tagaaggtga atagaattgt cagttataaa aatagaaaaa
    33121 gcacttaagc tagataaagt aatatttgta gatgtgagaa ctgaaggaga atatgaagaa
    33181 gaccatatat tgaatgcaat caatatgcct ctttttaaaa ataatgaaca taatgacgtt
    33241 ggaacaattt ataagatgca gggaaaacat gaagcaatac aaaaagggtt tgactatgta
    33301 tcgtataaat taaaggatat atatttacaa gcagcagaac ttgcattaaa ttatgataat
    33361 attgtcatat attgtgcaag aggcggaatg agaagtggca gtatagtaaa tttattatca
    33421 tcattaggtg taaatgtata tcaacttgaa ggtggatata aggcttatag aaattttgta
    33481 ttagagtatc taaggcatgt aatggataca aaaaactttg tatctgttca tggattgact
    33541 ggtacaggaa agactgattt gttacattta ttagatgaaa aaggaataga tgtactggat
    33601 ttagaaggca tagctaaaaa cagtggttct acctttggtt ttataacttt tgataaaaag
    33661 ccaccttctc aaaagaactt tgaaacaaaa ctatttgaaa gtatattttt ttcgaaagag
    33721 aattatatat ttgtcgagag tgaaagcaaa cgagttggaa gtgttacagt tccaaatgaa
    33781 attttcgagg caatgacacg agaaggacat catatattat tagaatgtga tattgaaaat
    33841 agagttgata gactttgtag agattatatt tatgataaag atgaaggtaa tatattgatt
    33901 ttaaaagaat gtataaacaa gtttagaaaa agattgggta ataaagtagt agatgattat
    33961 attgatttat tagaatcagg tgaatataaa gaacttgtaa aaagatatct tttagaatac
    34021 tacgacccac tttatatgca ttcagtagaa aaatataagt atagtaagat tataaatttt
    34081 aatgatacac aaaaagcttt agaagatgtt atagacttat atgagagtat attggaagga
    34141 gaaagtgaat gttaaaaata gcattagaat cattaggatg ctcaaaaaat ttagtagatg
    34201 cagaaataat gatgggaatt ttaaataaca aaggctataa attaattgga gattttgaag
    34261 aagctgatgt aattatagta aatacatgtg gatttataga gtcagctaaa caagaatcta
    34321 tagatacgat aataaacttt gcagagttaa aaaaaactgg caatttaaag ttgttgatag
    34381 taacagggtg tctagcccaa agatattctg aagaattaaa aactgaaatt cctgaaatag
    34441 atgctatagt aggaacagga agctatcaaa atatagataa aatacttaaa gaattaagtg
    34501 aaatgcataa aattgtaagc cttaatgata tagagtttgt atttaatgaa gatttaccaa
    34561 gatatatatc tacacctagc tatatggcct atttaaaaat tggagaagga tgttccaata
    34621 attgtactta ttgtataata ccaaaactaa gaggtaaata tagaagtaga aaatttgaag
    34681 atataattaa agaagctaag aaattagctg aatctggagt aaaagaactt gtggttatag
    34741 cacaggatac gacaaagtac ggttttgatt tatatggaaa agaaagatta tcagaacttt
    34801 tagaagaact tgcaaaaata gatggattta agtggataag ggttatgtat tcatatccag
    34861 aatctataac agaagaattg atacaagtta taaagaaata tgataatata tgtagttact
    34921 ttgatatgcc aatacagcat gcaagtaaca atattttaaa attaatgaat agaaaaacta
    34981 ctaaagaaga tatattaaat aaaatcaatc tgataagaag taatatacca gatgctatac
    35041 ttagaactac aataatagta gggtttcctg gtgaaacaga agatgacttc aaacaacttg
    35101 tggactttgt tgaagaggtt aaatttgata gacttggtgc atttgcatat tcaagagaag
    35161 aagatacacc agcagataga ttgccaaacc atatagatga agaagtaaaa atacaaagaa
    35221 gagatacttt aatgatgatt caacaaaaaa tatctgaaga acttaatgac aaaaagatag
    35281 gaaaaactta tgaggtgtta atagaagaac agatagaaga taatgtatat acaggaagaa
    35341 cacaaggtga tgcagaggaa attgacagta tagtgtatgt aaaaagtgta gacaatcttg
    35401 aagtaggaga atttgtaagt gttcaaataa atgatgcaat ggaatacgat ttaatgggag
    35461 atgttttata tgaacttgcc taacaagtta actttgttta gaatattttt aataccagta
    35521 tttgttttaa ttatgttgct caatatacca aataaatttt taatagcttg tattattttt
    35581 ataatagctt caattactga tgctttagat ggaaaaatag ctagaaaata caatctagtc
    35641 actgactttg gaaaatttat ggacccatta gcagataagt tattggttat ttcagcactt
    35701 acatgtatga tagaagacca tcttgtaagt agttggatgg taattataat agtggctaga
    35761 gagcttacag ttagcatatt aagagctata gctgctgctg atggaaaagt aattgctgct
    35821 ggaaacagtg gaaaacttaa aacgataact caaatggtat ctatagtgtt tttattgtta
    35881 ggtgctcagt ttgaaaatgt attgatatta aatattggtg aaattctcat tcttatagct
    35941 acactactta cattatattc tggatgggaa tatctatata aaaataaaga gctttttatg
    36001 tcttctaagt aaaaaattat gctttaataa ataaaaacta atgattaata aataaaaaat
    36061 aacaacttaa aattttaaaa atgtaaaaaa gggtatacta ttatagtata cctcttttca
    36121 ttttaagaat attttctttt gtgtatataa ttattaataa tggtttataa ataaaatgta
    36181 atgaataaaa atataatcat tagtagcgtt tttaaattga ctaggtttga tttatgttat
    36241 ataattaata tataacaaga gaacaaatgt ttgtagataa atgaagggat ggtatagaga
    36301 tgagtgtaga tcaagaaaaa ttaaaagcgt tgaatgaagc tttaggtaaa attgaaaaag
    36361 attttggtaa aggttcagta atgaaattgg gagaagcaac atctatgtct atagatgtta
    36421 tatcaacagg agcgattggt ttagacatag ctattggtat aggaggtcta cctagaggga
    36481 gaatagttga agtatatggt ccagaatctt ctggtaagac tactgttgcg cttagttgtg
    36541 tagcatcagc tcaaaaagat ggaggaatag ctgcatttat agatgcagaa catgcacttg
    36601 acccagtata tgcaaaagct ttgggtgtgg atgttgataa cctaataata tctcaaccag
    36661 atacaggtga acaggcttta gagatagcag aggcattgat aagaagtgga gcgatagata
    36721 taatagtaat agactcagta gcagcattag ttccaaaggc tgaaatagat ggagatatgg
    36781 gtgattctca tgtaggttta caagctagac ttatgtcaca agcacttaga aagttaactg
    36841 gttcaattaa aaaatcaaat tgtgttgcta tatttataaa ccagttaaga gagaaagtag
    36901 gaataatgtt tggaaaccca gaaactacta ctggaggacg tgcactaaaa ttctattcat
    36961 cagttagatt ggatgttaga aaaatagata caataaaaca aggtgataaa gttataggta
    37021 gtagaactag agttaaagtt gttaaaaaca aagtagcacc accatttaag caagctgaat
    37081 ttgatataat gtatggagaa gggatttcaa aaattggaga ccttttagat atagctgctg
    37141 atgtagatat agttaaaaaa tcaggttcat ggtatagtta caatgatact aaacttggac
    37201 aaggaagaga aaatgttaaa aaattcttgg aagataattt agatttaact actgaaatag
    37261 atgagaaagt tagagcattt tacaatttaa atgaagaaca tgaagaatca ggtacttcag
    37321 tatcaaaaga aattgtagaa gaataagtaa aaatacaaaa aaataactag taattaaaga
    37381 catgccacgc tccctattag cttgacacaa tgttgaaaaa attataaaat taagtgagga
    37441 taaaatatct aaatttaatt ttaattatta ggaatatttt ttaatcatag tgatgacatt
    37501 ttgggattat taacgaagga ggtggatgtc atagatagtg tagtaatgat tgtaataggt
    37561 gcagctgttg gtattatagc tggatatttt gttagaaaaa atatatctga agcaaaaatt
    37621 ggacaagctg aaaacttggc caaagaaata atagataaag cacaccacga ctcagaaaca
    37681 gtacaaaaag aaaaattact agaagctaaa gaagagatcc ataagtggag aacagaagct
    37741 gaaagagaaa acagagaaag aagaacagaa gtacaaaagt atgaaaaaag agtagtacaa
    37801 aaagaagaag tcttagatag aaaattacaa aatttagaat ctaaagaagt aaacctaagt
    37861 gaaaaattaa aggtagtgga aaagaaagaa gaagaagttg aagtaataaa aactcaacag
    37921 ttagaaaaat tagaaagtat atcaggaatt acttctgata aggccaaaga aatcatctta
    37981 accaatgcgg agagagatgt aagacgtgaa atgtctataa tgataaaaga gatagaaagt
    38041 caagctaaag aagaagctga taagaaatca agagaaatta taggatatgc aattcaaaaa
    38101 tgtgcagcag atcacgtagc cgaaactact gtaacagtag taagcttgcc taatgatgaa
    38161 atgaaaggta gaataatagg tagggaaggt agaaatataa ggactttaga aaccttaaca
    38221 ggtatagacc ttataataga tgacactcca gaagccgtaa ttttatcagg atttgatcca
    38281 ataagaagag aaattgcaag aattgctcta gaaaaattaa tagcagatgg aagaatacat
    38341 ccagctagaa ttgaagaaat ggttgaaaag gcgagaaaag aagttgacaa tattataaaa
    38401 gaatatggtg aacaagctgc atttgaaact agtgtacatg ggattcatcc ggaattaata
    38461 agattattag gtagacttaa ctatagaact agttatggac aaaatgtact taaacattct
    38521 atagaagttg ctcatatagc tggtataatg gctgctgaaa taggagcaga tattagattg
    38581 gctaagaggg caggtttatt acatgatata ggtaaggctg tagaccatga aatggaaggt
    38641 acacatgttg aaataggtat ggatttactt agacgttata aagagtctaa agaagttata
    38701 catgccatga gtactcatca tggagattac gagcctcaaa cagtagaggc tgtattagtt
    38761 acagcagcag atgcaatatc agctgctaga cctggtgcga gaagagaaac tttagaagca
    38821 tatataaaga gacttgaaaa attagaagaa atagctaatt catatgaagg tgtagaaaaa
    38881 tcatttgcta tacaagctgg tagagaaata agaataatgg taaaaccaga agctataaat
    38941 gatgaagaaa tacatctatt ggctagagat atgactaaga aaatagagga tgaacttgaa
    39001 tatcctggac aaataaaagt aagcataata agagaaacaa gagctataga gtatgcgaag
    39061 taaaagaaat aatatctaaa taataagaaa atatttttag aaatttaaat ttgccacaag
    39121 atatgataat atatcttgtg gcttttaaat tgtttaagtt taattttata taatgcaaaa
    39181 ataatctaag tgctaaaagt gctacttaca aaagattaa
//
LOCUS       DAYAOX010000012        39081 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.012, whole genome shotgun sequence.
ACCESSION   DAYAOX010000012 DAYAOX010000000
VERSION     DAYAOX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39081
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.012"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(8..1366)
                     /locus_tag="ACGI57_000493"
     CDS             complement(8..1366)
                     /locus_tag="ACGI57_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9715581.1"
                     /translation="MLRVALYIRVSTEEQALNGDSIRTQIEALEQYSKENDFNIVGKY
                     IDEGCSATNLKRPNLQRLLRDVEKDKVDLVLMTKIDRLSRGVKNYYKIMETFEKHKCD
                     WKTILENYDSSTAAGRLHINIMLSVAENEAAQTSERIKFVFQDKLRRKEVISGTIPIG
                     YKIENKHLVIDKEKKYIVKAIFDEYEKSGSVRTLIETINNLHGELYSYNKIKNILRNE
                     LYIGIYNKRGFYVEDYCEPIISKKQFKQIQRILEKNKKTTPNKNIHYHIFSGLLKCKE
                     CGYTLKGNSSNVGEKLYLSYRCSTFYLNKNCVHNVTHNEKHIENYLLTNLKPQLHKHM
                     VKLEAQNEKIRQNKKSNKKDEKKKIMKKLDKIKDLYLEDLIDKETYRKDYEKLQSQLD
                     NITEEQESQIIDTSHIKKFLDIDINEMYSDLSRVERRRFWLSIIDYIEIDNNKNITIN
                     FI"
     gene            complement(1431..1904)
                     /locus_tag="ACGI57_000494"
     CDS             complement(1431..1904)
                     /locus_tag="ACGI57_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFG9715582.1"
                     /translation="MNSYEKLLSEADDNNIIVREVSLISNSHGLYKNNRIALNKNTLN
                     NMSEKACVLAEELGHHYTSYGNILDLNKVENSKQEYKARLMAYNKLIGLKGIIDSFNA
                     GCKTITEMAEYLDVTEKFLNEALECYKSKYGFSATLDNYVIFFEPRFSIMNANFL"
     gene            complement(1922..2458)
                     /locus_tag="ACGI57_000495"
     CDS             complement(1922..2458)
                     /locus_tag="ACGI57_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9715583.1"
                     /translation="MKNTGHIIKILRIEKKLTQDELAKNLNSSFNLKLNKGMISKWES
                     NKAEPRFEYIKYISKYFDVSIDYLLGMTEYRNMYDNFDKKYDSKKLSLEVKEIEKNGE
                     KIKEKNKVLENKLLSSFSELNDIGQNEAIKRVDELTQIGKYVNKNHIDTIAAHNEHLH
                     EEGEIEKIYQDLDDMDNW"
     gene            2621..2830
                     /locus_tag="ACGI57_000496"
     CDS             2621..2830
                     /locus_tag="ACGI57_000496"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9715584.1"
                     /translation="MKSKRNPYLKFKIFLMERGIKQSELCKILGKSASAINQNINGTG
                     GDFSVEDLRIISQTYNISIDEFFIN"
     gene            2893..3783
                     /locus_tag="ACGI57_000497"
     CDS             2893..3783
                     /locus_tag="ACGI57_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFG9715585.1"
                     /translation="MNEVVNAENINEIEVIGITEVDGMKFHDIEGGFGECKKAMLVKE
                     IAEIHNKELKHINELINKNRKRFQNDIDIIDLLGVGLNDTKIKKFGFTQQAINSYRGS
                     KNKGLLTGIYLLSERGYAKLLKILEDDKAWEQYEKIVDGYFNMRKSLKENNKFNSSKP
                     KDIKDESEIKYMNAQARLKNARAREAKIYLELADKVDIKEYKQIMYSKTTELLSGETL
                     IPLPKMEKKTYSATEIGKILGISANKVGGLANAYNLKTDEFGIKVWDKAKHSNKQVPN
                     FRYYENVMPILEKALSSFEN"
     gene            3840..4439
                     /locus_tag="ACGI57_000498"
     CDS             3840..4439
                     /locus_tag="ACGI57_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715586.1"
                     /translation="MKENNRIVSVGTESEIDLTVKELNMIDDFKKIINGVNNEIANDY
                     SILEMDRDILEKIALSTALNYKKMNFEYSEIDEKLFNDFTFEFMLKFFGDIEEDELIK
                     YLVYIGEEEESYELNDRERIFYKIVDTLDTIADIKIADQNKIEKEVGMCIGEDNYITY
                     CIDENLIKFYIKDEEELVIDKDSPLLYMLDTLFYEVHEE"
     gene            4491..4679
                     /locus_tag="ACGI57_000499"
     CDS             4491..4679
                     /locus_tag="ACGI57_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715587.1"
                     /translation="MGKKSEYTVTTVGEPNLDALGETLAKILTEKFGVLITHTYIENY
                     YVDKERGGDVDGAIESEF"
     gene            4654..4881
                     /locus_tag="ACGI57_000500"
     CDS             4654..4881
                     /locus_tag="ACGI57_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715588.1"
                     /translation="MELLKVNFNLEKLKIGNVVSVSSKRFGFNINCIVVVATEEELNL
                     AYYSKDKGCIEYQALIPEDIRYGDYILQRLG"
     gene            5031..5204
                     /locus_tag="ACGI57_000501"
     CDS             5031..5204
                     /locus_tag="ACGI57_000501"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715589.1"
                     /translation="MLEVNIKIKGEASEIIDSIRAIFNSFGEESMTEIKAEQEYKSRR
                     SRKLKTEDLSDQE"
     gene            5220..6473
                     /locus_tag="ACGI57_000502"
     CDS             5220..6473
                     /locus_tag="ACGI57_000502"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFG9715590.1"
                     /translation="MQLYKHQEEALKLTEYNNKCAYYLDMGLGKTFVGSEKLINLGSN
                     TNLLICQKSKINDWVYHFKTYYPDMGIINGTKKLSEGIEYFKIVEVPFIVVINYELAF
                     RRKELLDLKDFTLMLDESSMIQNEKAKRSKFVLSLNPKNVILLSGTPCSGKYEQLYSQ
                     IKLLGWNISKDLYWKQYIDVEYKDIGGFPMKVVTGYKNVDRLKKKLRDYGAVFMMSDE
                     VFDLPKQIDNVMKISTTREYRKFKKDSIISISDDVELVGDTTLTKMLYERQLCSQYNK
                     FKLEAFGDLINSTGDRLIVFYNFNEELDRLIELSRDRPISIVNGSTKDLNNYEEHNNS
                     ITFVQYQAGAMGLNLQKCNKIVYFSLTLSCELFMQSKKRIHRIGQENTCFYYYMICRN
                     SVEENIYKSLQSGVDYTNNLFEKGN"
     gene            6475..6909
                     /locus_tag="ACGI57_000503"
     CDS             6475..6909
                     /locus_tag="ACGI57_000503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715591.1"
                     /translation="MIKCNEICDFKGENICCSECGDNETCSNICGLECSTCGKTIIAD
                     DAGLEEFRDNNYVLFKNIFNICEQKKSLEIEEKKMKEELKKSMELYNIKSFDNDILKM
                     IYIEASTTTTIDSKKLKEKYPEIYSDCSKISNKKAYIKISLK"
     gene            6918..7220
                     /locus_tag="ACGI57_000504"
     CDS             6918..7220
                     /locus_tag="ACGI57_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFG9715592.1"
                     /translation="MSAEKQFENKVKKFLKEQGCWFIKYWGGSAYTKSGIPDLLVCCS
                     GKFIGIEVKGEKGKASELQKYNIKEIEKAGGIGIILYPKDFDRFKKMILEIKKEGE"
     gene            7221..8906
                     /locus_tag="ACGI57_000505"
     CDS             7221..8906
                     /locus_tag="ACGI57_000505"
                     /inference="COORDINATES: protein motif:HMM:NF024870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9715593.1"
                     /translation="MQFSHSRIGVFKNCPYKYKLQYIDKVKTILNADSNNALILGTSL
                     HTGIEKDIDAAVKEYYFSYPSITDLHINEVMKLDYLIPKVKEILPKGFNEVQITTSDF
                     IGFIDLLAPHVYMTDTNLNTNETTIYYEDEERVFDIYDFKYSNNIDRYLESEQLHLYK
                     YFYEKQHPNHEIKDLYYVFVPKVQIRQKKTENLHQFRQRLLNELDKSEIKIVKVEYEP
                     NKVINFLVDIKRCLENKEFEKNQNKLCNWCDYQEYCEKGIDYMLLPNSERRSVEGINK
                     KTLWIYGAPFSGKTTLANDFEKPLMLNTDGNIKFVDAPYISIKDIVTVEGRMTKRQLA
                     WDVFKEAIRELEKKENDFETIIVDLLEDTYEACRLYMYDKMGITHESDDSFRAWDKVR
                     TEFLSTIKRLMNLDYKNIILISHEDTSKDITKKGGDKITAIKPNLQEKAANKIAGMVD
                     IVARVVADGDVRTLSFKTNEVIFGGGRLKVKSNEIPLDYNELMKVYEDISIEKNEIKK
                     SSKPKKSKTNEEVEMTPIVEETSEKTEKEQTEEEVIEQEEAIKPVKKTRKKRN"
     gene            8970..9413
                     /locus_tag="ACGI57_000506"
     CDS             8970..9413
                     /locus_tag="ACGI57_000506"
                     /inference="COORDINATES: protein motif:HMM:NF016896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFG9715594.1"
                     /translation="MDFSKFDKAIDVKGLKEDIKEASENSGKFKDVPHGTYEVEINKM
                     ELSESKKGDPMFVCWFKILEGEYKDSLIFMNQVVKQGFQIHIVNEFLRSLETDIEVEF
                     ESYSQYAQLIMDIAEEIDGELEFAIEYGEKKGFNTFTIKDIFEVA"
     gene            9498..9626
                     /locus_tag="ACGI57_000507"
     CDS             9498..9626
                     /locus_tag="ACGI57_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715595.1"
                     /translation="MELISPGKIVRCVWCGKRFYKLDNSKVIYCSRKCAAKARGVF"
     gene            9627..11360
                     /locus_tag="ACGI57_000508"
     CDS             9627..11360
                     /locus_tag="ACGI57_000508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715596.1"
                     /translation="MIFYDFEVFSYDWLVVFIDVLNKKEEVIVNDIDKLNSFYIEHRE
                     DIFIGYNSRHYDQYIFKGLLCGFNAKEINDYIIVKGQPGWKFSNLLRNIQINNYDVMT
                     SFHGLKQLEGFQGHSIKESNVSFNIDRPLTNDEIEETIKYCRYDVEQTIDVFIERKSE
                     FEAHMGLIKAFKLPVSYIGKTQTQLTAIILEATKKEHDDEFDLQIPDTLKIEKYKEVL
                     SWYKNPLNHDYSKNLKINISNVPHVFAWGGVHGAITKYYGEGYFLHVDVNSFYPSLMI
                     RYNYHSRNIKNPQKYVEIYDKNLQLKKEKSPLRPAYKLAVNKTYGGMKDKNNNLYDPR
                     QANNVCVSGQLLLLDLIEKLEGYCKLIQSNTDGLIVKLNTIDDYELIDDICYEWEQRT
                     GMGLGFDVYTKIFQKDVNNYLVITEDGEVEAKGLYIKDLGNLDYDLPIINKALKNYMV
                     NNTPIEETINKCNDLIEFQKIVKISSKYSHGLYSPTIEGKTKKVFIGGKILNDKCFRV
                     FASKNKNDGGIYKVKNKEKNPEKFANTPDKCFIENGSVIGKKVPRKLDKTWYIDLANE
                     RLKQFGVLLSN"
     gene            11372..13255
                     /locus_tag="ACGI57_000509"
     CDS             11372..13255
                     /locus_tag="ACGI57_000509"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFG9715597.1"
                     /translation="MSFDIFKGYIITNNKKAAEKFKNVKKLKTYEQIKNLPEFAGVLA
                     EETVLVDIDDFESSEILYKIVQDLKLKCRVYKTTRGKHFLFKNTSLEKNRTKCRLAIG
                     LNSDIKLGCKNSYSILKFNNIEREILYDSKEIQEIPMYLTPIKNGIDFLSLGEGDGRN
                     QALYNYILTLQSNDFTVEEIRETIRVINKYVLKTPLQDNELEVILRDESFQKKIFFNS
                     KGSFLFDEFAKYIKNNNHVIKINDQLHLYKDGIYVDGQARIEAEMINNISNLNKAKRS
                     EVLSYLNLLISENTSMSEANLIAFKNGIYNIVDDSFIEFSPEFIITNKVNWNYNPGAY
                     SKLVDKTMNKLSCGDFEIRMLLEEVVGYCFYRRNELRKAFILTGDKANGKSTYLDMIK
                     TLLGDENTSALDLKELSDRFKTAELFGKLANIGDDIGDEFIANPAIFKKLVSGDRVNV
                     ERKGQNPFDFNNYSKFLFSANNIPRIKDKTGAVLDRLIIIPFNASFSVNDKDFDPYIK
                     YKLREQEAIEYLINLGLEGLKRVLKNRKFTVPDKVKKEILEYEEVNNPILGFFKEVDK
                     IENESTKEIYRKYQEYCILNGLQPISNIEFSRQVVKKFGYEVKDKRIQGKKYKVFTRA
                     VPG"
     gene            13622..14029
                     /locus_tag="ACGI57_000510"
     CDS             13622..14029
                     /locus_tag="ACGI57_000510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFG9715598.1"
                     /translation="MTDYEKLEELLNSYSNLDIEIREIELKVRGSSLRGIELNGMPKG
                     NNVSSPIENELIYVERLENEKIYLQIKKESINNMLNLLDDFEKNLIELRYFKKLQYKQ
                     ISYELNISELYVGKKRKKALDKILPFAKKYNLI"
     gene            14218..14640
                     /locus_tag="ACGI57_000511"
     CDS             14218..14640
                     /locus_tag="ACGI57_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFG9715599.1"
                     /translation="MAKLTEKQKRFCDYYIETGNATEAAIRAGYSEKTAKVIGAENLT
                     KPYLKSYIDERIGQLESNRIADAKEVMEYLTKIVRNEAKEEVVVVSEYGPEIIKKDVS
                     IRDRNKAAELLGKRYRLFTDKVEVEGTGIVQIVDDIDE"
     gene            14633..15898
                     /locus_tag="ACGI57_000512"
     CDS             14633..15898
                     /locus_tag="ACGI57_000512"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFG9715600.1"
                     /translation="MNEIRLKNIIAPSFYNVHKLIKQDVYTHYWFKGGRGSTKSSFIS
                     LEIVLGMMRDAQQGKLTNAVVIRRVKDTLRGSVYEQIQWAIYTLGVQNEWEIPESKLQ
                     MTYKPTGQVILFKGADKPKKLKSTKVSKGYIKYVWYEEVDEFEGMDKIRNVNQSLLRG
                     GNTYNVFYSFNPPESQRNWTNMEVIEKREDKFIHHSNYLSVPKEWLGEQFIVEAEHLK
                     KVNPTKYEHDYMGVVTGTGGEVFTNVTVREISNEEINRFDRVRRGLDFGYAKDPLSYI
                     VMNYDKTRKRLYIFFELYKVALGNSKAVELILKENTSNKKVIADSAEPRSINEFKKLG
                     LKIIGARKGPDSVEHGIKFLSEEVEEIIIDPIRCPNAKREFLGYELEKDKEGNFKDEY
                     PDKNNHTIDAVRYGMEDVMLKRKGLSILK"
     gene            15914..17299
                     /locus_tag="ACGI57_000513"
     CDS             15914..17299
                     /locus_tag="ACGI57_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9715601.1"
                     /translation="MNIEVVKKLIKKHVPYHSDVISKSIISERYYKNKNDILFKTSNR
                     EEEETENPLRNADNRISSSFYSLLVNQKASYLFTYPPTFDVSNNRTNKAITEVLGDIY
                     PKICKDLCINASNSGVSWLHLWKDKNSKLNYGVVDSKQVIPLWSSDLNKKLIGVLRVY
                     DEIDENGDNYIIYEYWTDTECQAYRKLDDLDIDELEVYNMFSITYGNGDILDNSNTLK
                     HDFGELPFIPFFNNNIKTSDLDTVKSLIDTYDKVFSGFVNDLEDIQEIIFILTNYGGT
                     DLKSFLEDMKKYKTVEVESNGMDDKSGLSTLTIDIPVEAREKLLMMTRKAIFEQGQGV
                     DPQPIDFGNASGVALKYLYSLLELKAGLMETEFKLSFGKLIRMICKVLNLECNIVNQT
                     WTRNAIANDLELAEICAKSVGIVSDKTVCKNSPIVEDVEYEIAQIKKEKEEAQREYDD
                     LIPNDGVIDET"
     gene            17289..18776
                     /locus_tag="ACGI57_000514"
     CDS             17289..18776
                     /locus_tag="ACGI57_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFG9715602.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSIKYYLELEKQYKLAMSSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKVISKPWATDGLNFSERIWGKHRPALINELYTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKVFDMKDYQVGVTAPPFHCRCRTTTAPFFSDEEGYRAARGEDGKTYYVPSSMKYK
                     EWYEKYINKNIKLSDNEQLAINKYVSSDSYKINEKLRRGLSLTSEDKNFISNLDSALD
                     KMPNYKGNVNRSLYFFNEKEKIDFLNKHQIGKEIVYNEYISTSSRGEYNPSGQVELNI
                     ISTNGKDIRKYNPQEAEILFKRNSKFITTDNFEYDGKHYITMKEV"
     gene            18778..18936
                     /locus_tag="ACGI57_000515"
     CDS             18778..18936
                     /locus_tag="ACGI57_000515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715603.1"
                     /translation="MKKDNEKLFSAPRWTEIPQAKVVGERTLTEEEVKEAQEIRKKII
                     KRKINNKG"
     gene            19007..19252
                     /locus_tag="ACGI57_000516"
     CDS             19007..19252
                     /locus_tag="ACGI57_000516"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715604.1"
                     /translation="MIKLYILSIIVFCTGLYLFKMKIDSNEELIKILKNKKVRRKCNF
                     IFMALFPLLNFILGVIFIISSLLVSNEDIIKNLKGDK"
     gene            19254..19742
                     /locus_tag="ACGI57_000517"
     CDS             19254..19742
                     /locus_tag="ACGI57_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715605.1"
                     /translation="MAKFVKKPVEVEAFKLGYDVEPKWFIENDRVCNFIKEKCINGHV
                     SCDLETLEGTMRANKGDYIIQGVKGEIYPCKADIFEMTYQKVEYRGKNKLSTEMTLDS
                     TNFQENIKNATKELEFFIETLEKADDKIDKLAEKMNKCDCKVDIDKIVKQLGECLRES
                     IE"
     gene            19761..20057
                     /locus_tag="ACGI57_000518"
     CDS             19761..20057
                     /locus_tag="ACGI57_000518"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715606.1"
                     /translation="MFKKDTRGYIKRHIGKPNKSSVRDIISVLIIIVGIALGFIVGLS
                     IIIANVLDVANMIDTNTFTCMKLVFNLAGMIAGYLVGRIICFVLSLTGIFIGSK"
     gene            20121..20711
                     /locus_tag="ACGI57_000519"
     CDS             20121..20711
                     /locus_tag="ACGI57_000519"
                     /inference="COORDINATES: protein motif:HMM:NF018515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HFG9715607.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKDKHNNDLEKLKKDMEISKKEYELKGKLKDLG
                     VVDADYIIYKQGGTEKFNFDNEGKVIGLEDTIKVYKENSPHLFTGGKPNYIPKGGDDY
                     KGSNPWLEKSFNLTEQSKIYNDNPALAKELMTAAGK"
     gene            20728..21786
                     /locus_tag="ACGI57_000520"
     CDS             20728..21786
                     /locus_tag="ACGI57_000520"
                     /inference="COORDINATES: protein motif:HMM:NF039452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFG9715608.1"
                     /translation="MNKNKSLKMNIQLLAGNEGGTKLSDIIVPSLFNPYVINRTMEKS
                     ALLQSGIITNNSEFDKLASQASPLVNMPFFEDLTGESEQIIEDVDLQNNKITSNADVA
                     VIMRRAKMWSATDLSAALAGKDPAGAIAELVSGFWARDMQKELIAILKGVFGTTKAVG
                     ETVAETRLASNILDISSSSGNAAKWSGSAFIDAQQKLGDAKETLTAVAVHSAVEAELR
                     KQNLIETVQPSNSGPIYFYQNKRVIIDDGCPYEGSGSNIVFTSYLFGEGAIALGNGNP
                     VGFVATEIDRDKKKGSGVDYIINRKTFIMHPRGVKFTNENMAKTEGPSRAELSDATNW
                     KPVYEPKQIRMVAFRHKI"
     gene            21789..22139
                     /locus_tag="ACGI57_000521"
     CDS             21789..22139
                     /locus_tag="ACGI57_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFG9715609.1"
                     /translation="MEYKIIKIKRLLGLNESDSSKDTILEFILEDVEEIVKNYCNVPT
                     IPEQLNSTILRMAIEMYKNESLGSEDVALGSISSISEGDTSISYRSSASEFKESLLKD
                     YKAQLNRYRKIRWK"
     gene            22142..22486
                     /locus_tag="ACGI57_000522"
     CDS             22142..22486
                     /locus_tag="ACGI57_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715610.1"
                     /translation="MDKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQP
                     CKLSYKNITSTTDEKVAKLVQTIKLFISPNINIKAGSKLIINDKEYVRSGESAIYPNH
                     QEIILELLKDKA"
     gene            22486..22911
                     /locus_tag="ACGI57_000523"
     CDS             22486..22911
                     /locus_tag="ACGI57_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFG9715611.1"
                     /translation="MARWGSVDFREFKRACRRMEKFTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGIAYARSLPVYKSGNNYIIEVINPTEYASYVEFGHRTKNG
                     KGWVKGQHFLTISEMELQGQIDKIIEKKLLILLKGVFDA"
     gene            22904..23341
                     /locus_tag="ACGI57_000524"
     CDS             22904..23341
                     /locus_tag="ACGI57_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFG9715612.1"
                     /translation="MLNNIIDGISIKLDKSFGNEYTIYSEDVEQGINESCFFIVPLNP
                     SKVSYPSGRELKKNSFDVHYFPKSNDKSFEINEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIVDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            23325..23510
                     /locus_tag="ACGI57_000525"
     CDS             23325..23510
                     /locus_tag="ACGI57_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715613.1"
                     /translation="MIILSKTLNKEDDYKFTKEQIVNSKKYVNRKDLLNAILKENELY
                     SFSEVEKIINNFMKGVS"
     gene            23511..24821
                     /locus_tag="ACGI57_000526"
     CDS             23511..24821
                     /locus_tag="ACGI57_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFG9715614.1"
                     /translation="MALGGGTFVTQNKVLPGSYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIAIAKYSGIRGNDLKIIVTTNIDDNAKFDVVTLLDNKKVDIQVAKVITDLQDNDYI
                     TWKKDATLEATAGLTFTNGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVGVNYTQIQLEEALKSGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
     gene            24843..25313
                     /locus_tag="ACGI57_000527"
     CDS             24843..25313
                     /locus_tag="ACGI57_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFG9715615.1"
                     /translation="MAQQIKARDTISASKAECFVTIKGKRYNFMQAINLEAKMEKNKS
                     EIPILGSTTKGNKSTGSKYSGNATFYYNTSIFRELLYEYKETGEDIYFDIQITNEDPT
                     SSVGRQTIILEDCNMDSGIIAKFDADGEYLDEDMDFTFENWKLVEKFNIANGME"
     gene            25366..25623
                     /locus_tag="ACGI57_000528"
     CDS             25366..25623
                     /locus_tag="ACGI57_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715616.1"
                     /translation="MIKIKDKYEIKDSISFDYSNKRPLEERVSEMYKKAGKYLIDISD
                     KLATDTIDGSSLKPIIIKFEINEAGVATIEKQTKYLVMEVE"
     gene            25628..26068
                     /locus_tag="ACGI57_000529"
     CDS             25628..26068
                     /locus_tag="ACGI57_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9715617.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKAIEWELKAIDSD
                     RDRQLRKDSTIRVPILNKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            26330..27121
                     /locus_tag="ACGI57_000530"
     CDS             26330..27121
                     /locus_tag="ACGI57_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFG9715618.1"
                     /translation="MGLFSKKNKKPCCICGSEKGLMPSIEGENFCTACNCKYIDFSEN
                     ILKVTSIMKMMSNSEGMKKFIEVEKKNLKLLEKFTETKSINSSISFDEEQNLLKISYK
                     NRNQILVEKIIKFDDILEFELLEDGETIVKGGLGRAITGGVLFGGTGAVVGGITGKKT
                     SRKVVEIFKIKITVKDINNPIEYINLINSKVKTNSSIYQKAFSDAQEILSILSIITKY
                     NVMEDKKKSISSSTADEILKYKNLLDMEAITQEEFDAKKKELLNL"
     gene            27188..29530
                     /locus_tag="ACGI57_000531"
     CDS             27188..29530
                     /locus_tag="ACGI57_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFG9715619.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMNNAMNIVLSSFEQLQRTSSNAID
                     ANSIRTAREELARAEAGFDRLEQQIRESDNQQRRLNEDINKGASSTDRLVGSAKKLAA
                     TYLGIRTLGGLGNLSDQMTSTNARLSMINDGQLSDGGLNKMIFQSAERSRASYLDTAK
                     IVSRIGMNAGKAFSSTKEIVGFAEQLNKKFVIAGASTEEMNSALLQLTQGLSSGVLRG
                     EELNAVFESAPNIIQSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAAAEQTNAEFE
                     KMPYTLGQIFTSVKNNAVMVFGAIQKKIEDTVSSRGFRTFITDVKDSLYVLGAVGFNV
                     FSGFINLLSSPAFQNFFNVMIVGTSLVVQGLGWIITQALSVANVFSRNWSIISPIILG
                     VVSAISTYIIALGIMQAVTIAGTVANWMSWTSEVIRQRGILGTARALAIGKAEQWGFN
                     AAIYACPIFWIVLGIIAVISAVFILVAVYNHFAGTSISAISLIVGAWYWLCAVVYNVI
                     VGIVNAINICVVEIANIFRTGLYAVQCFFIDMANAGLKAGVNLDKAFDKFATNLANGI
                     IKAVNIAVKGLNWLVQQINKIPGIDLPQMKEFQKVNTVIGTKTTFKPIQKPPEPKAWK
                     PELVEYKNLKSEFIKGYDVGHQLQNKFKDTFDISKIAEKAKKDLGLDDLWDKKYGLGD
                     GLGSAGLNSPLSDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFTGVN
                     IKIDMNNTNNISKDADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            29546..30247
                     /locus_tag="ACGI57_000532"
     CDS             29546..30247
                     /locus_tag="ACGI57_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFG9715620.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSREMGGKVLFN
                     TNMKVSLEEYNIVEDADNGSDVKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPTPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKTMMDKANKGKK
                     VPKYTIYKGQVLKLG"
     gene            30240..32213
                     /locus_tag="ACGI57_000533"
     CDS             30240..32213
                     /locus_tag="ACGI57_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9715621.1"
                     /translation="MADELVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMYVLYDEFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSTHMRSWGILQLFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIDLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSTTSKEDNDMINKLNKVFKNKLSNTGSIFVKYSNAYK
                     VNAALMAAISIHETGNGSSSLCKNKNNFFGMKGMSFGSVDEGIKRGISNLSRNYIHMG
                     RKTLESIRDKYAPLYDSPLNKHWVPGVNKFYKQITGNAYSSNSAGTGVRSNEEAEKNL
                     KDITYQVQNSNNANTSTNNNSKVNKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAF
                     KRGAGINLKRVSADQSNDSRGKLLCNINDVKAGDLVFFAYNQGKGNVHHVGLCIGNDK
                     FIHSPNTGDVVKISSLSGRQKQKHDFARARRFF"
     gene            32227..32487
                     /locus_tag="ACGI57_000534"
     CDS             32227..32487
                     /locus_tag="ACGI57_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HFG9715622.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMQIAFGTIESVNPLVVKIEQKA
                     SFEESFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            32492..32911
                     /locus_tag="ACGI57_000535"
     CDS             32492..32911
                     /locus_tag="ACGI57_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFG9715623.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     IENVDNFEFENIKGKVNCKFMVHTKYGNIKAERVVSV"
     gene            32911..33960
                     /locus_tag="ACGI57_000536"
     CDS             32911..33960
                     /locus_tag="ACGI57_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFG9715624.1"
                     /translation="MFEFMTFENIIKRMLDSVPSTLDKREGSIIYNALAPVAIELSET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATNTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISECIYKMKCETAGPIFELGQLIPIEYIDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPLQNQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWEN
                     IKLIAEEVIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDITNTMTNDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            33953..34570
                     /locus_tag="ACGI57_000537"
     CDS             33953..34570
                     /locus_tag="ACGI57_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFG9715625.1"
                     /translation="MDKEINLINYLPQVLQDKEEYIKVFNAENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSINPKSNETLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNSTYNIHLESDARNQDWFEEVHSFVSSIKPCNMSLDYTRVLVSKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDILTGNGVGYQEVTIF"
     gene            34582..35607
                     /locus_tag="ACGI57_000538"
     CDS             34582..35607
                     /locus_tag="ACGI57_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9715626.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSNVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKIELTSNIETAKTELNTRIDTENEKQNIKIDNIIAGGVNVSHTHIIE
                     VADWTLNNETNMYEVTINHPLLTKRILIALYDEIGEALTPNARAIDDNSILVRNEENI
                     KMYVYLINGNAETHFINATVDDNRVSEMTTYSSKKIEDRFLNLEEKVNGGISSIATSV
                     NELITYC"
     gene            35622..37298
                     /locus_tag="ACGI57_000539"
     CDS             35622..37298
                     /locus_tag="ACGI57_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine rich domain-containing protein"
                     /protein_id="HFG9715627.1"
                     /translation="MQTEWNFGYNGSPQSVILKPGKYKFECWGSSGGINNSSWYTDAK
                     GGYSKGEITLKKQTTLYVYVGESGFASSSTSNNTKSGFNGGGKGYLNQQIMGTYYSMY
                     GGGATDIRLVGGAWDNEQGLLSRIIVAGGGGGSYYPYTGGAGGGLAGGTGYSANDRHR
                     PGGTQYQGGIGRVSTENGSFGKGCSAKDSTGEGGGGGWFGGAGMNGAGAGGGGSGYVL
                     TKDSYKPTGYTPTSEYYFDNVVMESGGNTAGAYGYAKITLLQALPFLTVSSYNSITAT
                     FKADHTDPTLLTKIEYFIDDVLKETITTDLTTEKTINYTLEDNALHTLKIVVTDSNNA
                     TAEKVLSISKNIMPLPEDVNLQDISSKLIEVNAGFKTGKTSIINTLALKNIEANLNNT
                     LVELSEKIKTGFDSSDATAEQLQNQITNLNNQLSQRKRWAKGTYTFTQSDYENFYSRY
                     SSAENYAKSLTVPINLNFIPSLVIVEKLCFYGNGYTVNFYAINNLNQLASVGRVPKSS
                     GEYRFCAFMFVKTITSKEFSLYTYRDEYTDSSSVVYRCKVGESFTWYAYE"
     gene            37319..37612
                     /locus_tag="ACGI57_000540"
     CDS             37319..37612
                     /locus_tag="ACGI57_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715628.1"
                     /translation="MERGNRIICDQTGKILLQTGEATGDILEHDEITELHFVDIPYGS
                     IDYTRNRIIGINIETKEPILEEIPTFISDEEKRIQELENQILLNENEKVGGIL"
     gene            37612..37794
                     /locus_tag="ACGI57_000541"
     CDS             37612..37794
                     /locus_tag="ACGI57_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715629.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNRVFELDDVTNTEYRMAVEN
                     YIIEHSEVVEGAELTK"
     gene            37835..38065
                     /locus_tag="ACGI57_000542"
     CDS             37835..38065
                     /locus_tag="ACGI57_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFG9715630.1"
                     /translation="MNEELVKEKISTLETRVKEHGKQIDRIEIEQAKFAIQIQNLCSD
                     IKNLTGVLKWLVGVIITTLVGFFIFAIQKGIF"
     gene            38085..38342
                     /locus_tag="ACGI57_000543"
     CDS             38085..38342
                     /locus_tag="ACGI57_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFG9715631.1"
                     /translation="MDNLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYIPIILLVI
                     GIGFSIWMLGFNPSSILQGILCWGVAIGANQVYKQLKDGDK"
     gene            38342..>39081
                     /locus_tag="ACGI57_000544"
     CDS             38342..>39081
                     /locus_tag="ACGI57_000544"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9715632.1"
                     /translation="MKIAIVPGHTLSGKGTGATGYIDEGKENRILTDLIVKWLKQGGA
                     IVYTGKVDKSNNYLAEQCKIANRQNVDLAVQIHFNANKTTLNPMGTETIYKTNNGKVY
                     AERVNSKLSTVFKNRGAKSDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKTIDNKENGEGKIMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIK
                     DYVPGQTENLYVVGGGACEKISSMTKER"
BASE COUNT        15735 a         4136 c         7248 g        11962 t
ORIGIN      
        1 caaataatta tataaaatta atagtgatgt ttttgttatt atctatttct atataatcta
       61 ttatagataa ccaaaaacgt ctgcgttcaa ccctgcttaa atcactatac atctcattaa
      121 tatcaatatc taagaatttt tttatatgtg atgtatcaat aatttgactc tcctgttctt
      181 cagttatatt atccagttga gattgtaatt tttcataatc ttttctataa gtctctttat
      241 caatcaaatc ctctaaatat aaatctttga ttttatctaa ttttttcata attttcttct
      301 tttcatcttt tttgttagat tttttatttt gtctgatttt ttcattttgt gcttctaatt
      361 tcaccatatg tttatgtaat tgtggtttta agttagtcaa aaggtagttt tctatatgtt
      421 tttcattatg agttacattg tgtacacagt ttttatttag atagaatgta gaacatctat
      481 aagataaata aagtttttct ccaacattac tagaattacc ctttaatgta taaccacact
      541 ccttacattt taaaagacca gaaaaaatat gataatgtat gtttttatta ggagtagttt
      601 ttttattttt ttctaatatt ctttggattt gtttaaattg tttttttgat ataataggtt
      661 cacaataatc ttcaacataa aatcctcgtt tattatagat tcctatataa agttcatttc
      721 ttagtatatt ctttatttta ttataactgt ataattcgcc atgaagatta ttaattgttt
      781 ctattaaagt cctaacagaa cctgatttct cgtattcgtc aaatatagct ttaactatat
      841 attttttttc tttatcaata actagatgct tattttctat tttgtaacct ataggaattg
      901 taccacttat aacttcttta cgtctcaatt tatcctgaaa cacaaatttt attctctcag
      961 atgtttgagc tgcttcattt tctgctacag ataacataat gtttatatgt aatctaccag
     1021 cagcagtact agaatcatag ttttctaaaa tggttttcca atcacattta tgtttttcaa
     1081 aagtctccat tattttataa tagtttttaa cgccacgact tagtctatca attttagtca
     1141 ttaaaactag gtctacttta tctttttcca catctcttaa taatcgttgt aagttaggtc
     1201 tttttaaatt agttgcagag caaccttcat caatgtattt accaactatg ttaaaatcat
     1261 tctctttaga gtactgttct aaagcctcta tttgtgttct tatgctatct ccatttaaag
     1321 cttgttcttc tgtactaaca cgtatataaa gtgctactct taacatttta cattccccct
     1381 tattgtatat ttaagaggag ctgaacggct cttaaatata ctatgaattg ttacaaaaaa
     1441 tttgcattca taatactaaa tcttggctca aaaaatatca cataattatc tagtgtagct
     1501 gaaaaaccat atttactttt ataacattct aaagcttcat ttagaaactt ttcagttaca
     1561 tcaaggtatt ctgccatttc ggttatagtc ttacagccag cattaaaact atctattata
     1621 cctttaagac caattaactt gttgtaagcc atcaatctag ctttatattc ttgtttgcta
     1681 ttttcaactt tatttaggtc taatatattt ccatatgaag tataatgatg ccctagttct
     1741 tctgctaaca cacaagcttt ttcactcata ttattaagtg tatttttatt taaagctatt
     1801 ctattatttt tatataatcc atgagaattt gaaattaaag aaacttctct aacaataata
     1861 ttattatcat ctgcttcaga gagtaacttt tcataactat tcataagttc acctacctat
     1921 tttaccaatt atccatatca tctaaatctt gatatatttt ttcaatttct ccttcttcat
     1981 gtaaatgttc attgtgtgct gctattgtat ctatgtgatt tttatttaca tatttaccta
     2041 tttgagtaag ttcatctact ctttttattg cttcattttg tccaatatcg tttaactcac
     2101 taaagctaga taaaagctta ttttctaata ctttattttt ttctttaatc ttttctccgt
     2161 ttttttcaat ctctttaact tctaaactta attttttaga atcatatttc ttatcaaagt
     2221 tatcatacat atttctatac tctgtcattc ctaataaata gtctattgaa acatcaaaat
     2281 actttgatat atattttata tattcaaatc tgggttctgc tttgtttgat tcccattttg
     2341 aaatcatacc tttatttaat tttaaattaa aactagaatt tagattcttt gcgagttcat
     2401 cttgcgttaa ttttttttca attcttaaaa tctttataat atgacctgta tttttcatgg
     2461 attacacctc tttataattt agatatactt aaattatata acatgaggtt tcattgtcaa
     2521 tgatttaatt taaaaaaaat aaagaaagtt attgacaatg aaacttgcaa taatatataa
     2581 tttaggtatc aaatatgaaa caaagaaggg ggtgaaaaac ttgaaaagta aacgtaaccc
     2641 atatctcaaa tttaagattt ttttaatgga aagaggaatt aaacaatctg agttatgtaa
     2701 aatacttgga aagtcagcat ctgctataaa tcaaaatata aatggaactg gaggtgattt
     2761 tagtgttgaa gatttaagaa ttatatcaca gacatataat ataagtatag atgaattttt
     2821 tattaattaa aaagtttctt atatgaaaca agagctgttt aaaaaataca aaaaactagg
     2881 gaggaagaaa ttatgaacga agttgtaaat gcagaaaata taaatgaaat agaagtgata
     2941 ggaattacag aagttgatgg tatgaaattc catgatatcg aaggaggttt tggagaatgc
     3001 aaaaaagcaa tgttagttaa agaaatagct gaaatacata ataaagagtt aaagcatata
     3061 aatgagctta taaataagaa tagaaaaaga tttcaaaatg acatagatat aattgatttg
     3121 ttaggtgtcg gtttgaacga caccaaaata aagaagttcg gatttactca acaagcaatt
     3181 aattcttata gaggttcaaa aaataagggt ctattgacag ggatatattt gttatctgaa
     3241 aggggttatg caaagttact taaaatacta gaagatgata aagcatggga gcaatatgag
     3301 aaaatagttg atggatattt taatatgagg aagagtttaa aagaaaataa taaatttaat
     3361 agtagcaaac ctaaagatat taaggatgaa tcagaaataa aatatatgaa tgctcaagca
     3421 agattaaaaa atgcaagggc aagagaagca aaaatatatt tagagttagc tgacaaagta
     3481 gatataaaag aatataaaca gattatgtat tcaaaaacta ctgaattact ttctggagaa
     3541 acattaatac cacttcctaa aatggagaaa aaaacttatt ctgctacaga gataggtaag
     3601 atattaggga tttcagccaa taaggttgga ggattagcaa atgcttataa tttaaaaaca
     3661 gatgagttcg gaattaaagt ttgggataag gctaaacatt caaataaaca agtgcctaat
     3721 tttagatatt atgagaatgt aatgccaatt ctagaaaaag ctttaagtag ttttgaaaat
     3781 taaattcaga agttgctaag tacaacttta gaaacttaaa tttaaggagg aataagaaaa
     3841 tgaaagaaaa taacagaata gtaagtgttg gaactgaaag tgagatagat ttaacagtta
     3901 aggaattgaa tatgatagat gatttcaaga aaattataaa tggtgttaac aatgaaatag
     3961 ctaatgatta ttctatttta gaaatggata gagatatttt agagaaaatt gcattaagta
     4021 cagctttgaa ctataaaaaa atgaattttg agtattcaga aatagatgaa aaactattta
     4081 atgattttac ttttgagttt atgctaaagt tttttggtga tatagaagag gatgaattaa
     4141 tcaaatatct cgtttatata ggagaggaag aggaaagtta tgaattgaat gatagagaaa
     4201 ggatatttta taaaatagta gatactcttg atacaatagc agatataaaa atagcagacc
     4261 aaaataagat agaaaaagaa gtaggtatgt gtatcggtga agataattat ataacttatt
     4321 gcatagatga aaatttaata aaattctaca ttaaagatga agaagaatta gttatagata
     4381 aggatagtcc tttgttatat atgctagaca cgttatttta tgaagtacat gaagagtaaa
     4441 aattgcaatg attaaatacg gaatattttg aaaatagagg ggtgatttaa atgggtaaaa
     4501 aaagtgaata cacagtaaca acagttggtg aaccaaattt agatgcatta ggcgaaactt
     4561 tagctaaaat tcttacagag aaatttgggg tactaataac tcatacatac attgagaact
     4621 attatgttga taaagagaga gggggtgatg tagatggagc tattgaaagt gaattttaat
     4681 ctagaaaaat taaaaatagg aaatgtagtg agtgtaagtt ctaagaggtt tgggttcaat
     4741 attaattgta ttgtagtagt agcaaccgag gaggaactaa atttagctta ttatagcaaa
     4801 gataagggat gtattgagta tcaagcattg ataccagagg atattagata tggtgattac
     4861 attcttcaaa gactaggtta ggaggaaata aaatagcagt tttaataatg gtaggtttat
     4921 ttgcaatatg tttagtagga ttatttcaat aaaaaagtgc tggtcaaagt aaccaacaca
     4981 caaaataaaa aaagttaata aaattataac ataaaaagga gagatttaaa atgcttgaag
     5041 ttaatataaa aatcaaaggt gaagcaagtg aaataattga ctcaattaga gctatattta
     5101 atagctttgg tgaagagtca atgacagaga taaaagcaga gcaagaatat aaaagtagac
     5161 gtagtagaaa gttaaaaaca gaagatttat cagaccagga ataaagaagg tgatataagt
     5221 tgcaactata taagcatcaa gaggaagctt taaagctaac agaatataat aataaatgtg
     5281 catactacct tgacatgggg cttggtaaga catttgtagg aagtgagaaa ttaataaatc
     5341 taggaagtaa tacaaattta ttaatctgtc agaaatcaaa aattaacgat tgggtatatc
     5401 actttaaaac atattatcca gatatgggga taatcaatgg tacaaagaaa cttagtgaag
     5461 gcatagagta ttttaagatt gttgaagttc catttatagt agtaattaat tatgaattag
     5521 cttttagaag aaaagagtta ttagatttaa aagattttac tttaatgtta gatgaatcat
     5581 caatgattca aaatgaaaaa gctaaaagaa gtaagtttgt actaagttta aaccctaaaa
     5641 atgtgatact actttctggt actccatgta gtggtaaata tgagcaacta tattctcaaa
     5701 ttaaactact agggtggaat atatcaaaag atttatattg gaagcaatat attgatgttg
     5761 agtacaaaga tattggtggt tttccaatga aggttgtaac aggttataag aatgttgaca
     5821 gattaaaaaa gaagttaaga gattatggag cagtatttat gatgtcagat gaagtatttg
     5881 acttaccaaa acaaatagat aatgtaatga aaattagtac tacaagagag tataggaagt
     5941 ttaaaaaaga ttcaattata agtatttcag atgatgttga acttgttgga gatactacac
     6001 taactaaaat gttatatgaa agacaattat gtagtcaata caataaattt aaattagaag
     6061 cttttggaga cttaataaac tcaacaggtg acagattaat agtattttac aattttaacg
     6121 aagagttaga cagattaata gaattatcaa gagatagacc aatatcaata gtaaatggct
     6181 ctacaaaaga tttaaataat tatgaggagc ataataactc aattacattt gtacaatatc
     6241 aagcaggagc tatgggttta aatcttcaaa agtgtaataa gattgtatat tttagtctta
     6301 ctttatcttg tgagttattt atgcaaagca aaaagagaat tcatagaata ggtcaagaaa
     6361 acacatgttt ttattattat atgatttgta ggaatagtgt tgaagaaaat atatataaaa
     6421 gcttacaaag tggtgttgac tatacaaata atttgtttga gaaaggaaat taaaatgatt
     6481 aagtgtaatg aaatttgtga ttttaaaggt gaaaatattt gttgtagtga atgtggcgat
     6541 aacgagactt gtagtaatat atgtggctta gaatgtagta catgtgggaa gacaattatt
     6601 gcagatgatg caggattaga ggaattcaga gataataact atgttttatt taaaaatata
     6661 tttaatatat gtgaacaaaa gaaaagcctt gagatagaag aaaagaaaat gaaagaagag
     6721 ttaaaaaaat caatggaact ctataatata aaatcatttg ataatgatat tttaaaaatg
     6781 atatatattg aagcaagtac cacaactact attgatagta agaaacttaa agaaaaatat
     6841 ccagaaattt attcagattg ttctaagata tcaaataaaa aggcatatat aaaaatatct
     6901 ttaaagtagg tggttatatg agtgcagaaa aacaatttga aaataaagtt aaaaagtttt
     6961 taaaagaaca aggttgttgg tttattaaat attggggagg ttcagcatat acaaaaagtg
     7021 gtataccaga tttattagtt tgttgtagtg gtaaatttat aggtattgaa gtcaagggag
     7081 aaaaaggaaa agcaagcgaa ctacaaaagt ataatattaa agaaattgaa aaggcaggtg
     7141 gaataggaat tattctatac cctaaagact ttgatagatt taaaaagatg attttagaaa
     7201 taaagaaaga aggtgagtaa atgcagttct cacatagtag gataggagta tttaagaatt
     7261 gcccatataa gtataagcta cagtatattg acaaggtgaa aacaatatta aatgcagatt
     7321 ctaacaatgc tttaatatta ggaacatcac ttcatactgg tattgaaaaa gatattgatg
     7381 cagcagtaaa agaatattat tttagttatc caagtataac cgatttacac attaatgaag
     7441 tgatgaagtt agattattta ataccaaaag taaaagaaat tttaccaaaa gggtttaatg
     7501 aggttcaaat aacaactagt gattttatag ggtttataga tttgttagca ccacatgtat
     7561 acatgactga tacaaactta aatacaaatg aaacaaccat atattatgaa gatgaagaaa
     7621 gagtatttga tatttatgac tttaaatatt caaacaacat agatagatat ttagaaagtg
     7681 agcaacttca tctatataag tatttttatg aaaagcaaca tccaaatcat gagataaaag
     7741 atttgtacta tgtatttgta cctaaagttc agattagaca gaagaaaaca gaaaacttac
     7801 atcaatttag acaaagatta cttaatgagc ttgataaatc tgaaataaaa atagtgaagg
     7861 ttgagtacga accaaataaa gttattaatt ttcttgtaga tataaaaaga tgtcttgaaa
     7921 acaaggaatt tgaaaaaaat caaaataaat tatgtaactg gtgtgactac caagaatact
     7981 gtgaaaaggg gattgattat atgttattac caaatagtga aagaagaagt gtggaaggaa
     8041 ttaataaaaa aactttatgg atttatggag caccatttag tggcaaaact acattagcaa
     8101 atgattttga aaagccttta atgcttaata cagatggaaa tataaagttt gttgatgctc
     8161 catacatttc tataaaagat attgtaacag ttgaagggag aatgacaaag agacaacttg
     8221 catgggatgt atttaaagaa gctataagag aattagagaa aaaagaaaat gattttgaaa
     8281 caataattgt tgacttgtta gaagatacat acgaagcttg tcgcctttac atgtatgaca
     8341 agatgggaat aactcatgag tcagatgata gttttagagc atgggataag gttagaacgg
     8401 agtttttaag cactataaaa cgacttatga accttgacta taaaaacata atattaatat
     8461 cacatgagga tacatcgaaa gatattacca aaaaaggtgg tgacaagata acagcaatca
     8521 aacctaattt acaagaaaaa gcagcaaata aaatagcagg tatggttgat atagttgcaa
     8581 gagtagtagc tgatggagac gttagaacat tatcatttaa gactaatgaa gtgatatttg
     8641 gagggggtag attaaaagta aaatctaatg aaattccact tgattataat gaattaatga
     8701 aagtatatga ggatattagt atagagaaaa atgaaataaa gaaatcaagt aagcctaaaa
     8761 aatctaaaac taatgaagaa gtagagatga ctcctatagt agaagaaaca agtgagaaaa
     8821 ctgaaaaaga acaaacagag gaagaagtaa ttgaacaaga agaagcaatt aaaccagtca
     8881 agaaaactag aaaaaagaga aattagtttg agtgattata taagaaagat tattaattaa
     8941 aaatttaaaa gaaaaggatg gtatgtgata tggattttag taaatttgat aaagcaatag
     9001 atgtaaaagg attaaaagaa gatataaaag aagcatctga aaatagtggt aaatttaaag
     9061 atgtgccaca tggcacttat gaagtagaaa taaataaaat ggagttaagc gagtctaaaa
     9121 aaggtgaccc aatgtttgta tgttggttta aaatactaga aggagagtat aaagattcat
     9181 taatattcat gaatcaagta gttaagcaag gttttcaaat tcatatagta aatgaatttt
     9241 taagaagttt agaaactgat attgaagttg aatttgaatc atacagccaa tatgcacaac
     9301 tcataatgga tatagctgag gaaatagatg gagaacttga atttgctatt gaatatggtg
     9361 agaagaaagg ttttaatacc tttacaataa aagatatatt tgaagtagct tagaaatttt
     9421 attacagcta ggtgtataca tgcatctagc tgtaatatta aaaaggtagg taattaatat
     9481 gagagtaaaa agaaaaaatg gaacttataa gcccaggtaa aattgtaaga tgtgtttggt
     9541 gtggaaaaag attttacaag ttagataact ctaaagtcat atattgtagt agaaaatgtg
     9601 cagcaaaagc gagaggggtg ttctaaatga ttttttatga ttttgaagta ttctcttatg
     9661 actggttagt tgtatttata gatgttttaa ataaaaaaga agaagttatt gtaaatgata
     9721 tagataagtt aaattcattt tatatagagc atagagaaga tatttttatt ggttacaaca
     9781 gtagacatta tgaccaatat atttttaaag gattattatg tggatttaat gcaaaagaaa
     9841 taaatgatta tattattgtt aaaggtcaac ctggttggaa gttttcaaac ttattaagaa
     9901 acatacaaat taataactat gatgttatga ctagttttca tggattaaaa caattagaag
     9961 gttttcaagg tcatagtata aaagaatcta atgtatcatt taacattgat agaccattaa
    10021 ctaatgatga aatagaagaa actataaaat actgtagata tgatgtcgaa caaactatag
    10081 atgtttttat tgaaagaaaa tccgaatttg aagcacatat gggattaatt aaagctttta
    10141 aacttccagt atcatatatt ggtaaaactc aaacacaact aacagcaatt atattagaag
    10201 ctacaaagaa agaacatgat gatgaatttg acttacaaat accagatact ctaaaaattg
    10261 agaaatataa ggaagtttta agctggtata agaacccact aaatcatgac tattctaaaa
    10321 atctaaaaat taatatttct aatgtacctc acgtatttgc ttggggtggt gttcatggag
    10381 caataacaaa atactatggt gaaggttatt ttttacatgt ggatgttaat tccttctatc
    10441 caagtcttat gattagatat aattaccact caagaaatat aaaaaatcct caaaaatacg
    10501 ttgaaattta tgacaaaaac ttacagttaa aaaaagaaaa atcacctcta agaccagcct
    10561 acaaattggc agttaataag acttatggag gaatgaaaga taaaaacaat aatttatatg
    10621 acccaagaca ggctaataat gtatgtgtta gtggacagct gttattactt gatttaatag
    10681 agaagttaga aggttattgt aaacttattc agagcaatac agatggatta attgtaaagt
    10741 taaatacaat agatgattac gaattaatag atgatatatg ttatgagtgg gaacaaagga
    10801 caggtatggg tttaggtttt gatgtataca ctaaaatatt tcaaaaggat gttaacaatt
    10861 atttagttat aacagaagat ggagaagttg aagcaaaagg attatatatc aaagatttag
    10921 gaaatctaga ctatgattta ccaattataa ataaagcact taaaaattat atggtaaaca
    10981 atacacctat tgaagaaact ataaataaat gtaatgattt aatagagttt caaaagatag
    11041 taaaaataag tagtaaatat tcacatggtt tatatagtcc aactatagag ggaaaaacaa
    11101 aaaaagtatt tattggcggt aagattttaa atgataaatg ttttagagta tttgcttcaa
    11161 aaaataaaaa tgatggtggt atttataaag tcaaaaataa agaaaaaaat ccagaaaaat
    11221 ttgctaatac tccagataaa tgttttatag aaaatggaag tgttataggt aaaaaagtac
    11281 caagaaagct agataaaact tggtatatag atttagcaaa tgaaagactc aagcagtttg
    11341 gggtattatt aagtaattga ggtggtgatt ggtgagcttt gacatattta aaggatatat
    11401 aattacaaat aataaaaagg cagctgagaa atttaaaaat gtaaaaaagt taaaaactta
    11461 tgaacagatt aagaatttac cagagtttgc aggcgtttta gcagaagaaa ctgtgttagt
    11521 tgatattgat gattttgaaa gtagtgaaat tctatacaaa atagtgcaag atttaaagct
    11581 aaaatgtaga gtttacaaga caacacgagg taagcatttt ctatttaaaa atactagttt
    11641 agaaaagaat agaacaaaat gtagactagc tataggttta aattcagata taaaattagg
    11701 atgtaaaaat tcctattcaa ttttgaagtt caacaatatt gaaagagaaa ttttatacga
    11761 tagtaaagaa atacaagaaa taccaatgta tttaacaccg attaaaaacg gaattgattt
    11821 tttaagtctt ggagaaggag atgggagaaa tcaagcatta tataattata ttttaactct
    11881 acagagtaat gattttactg ttgaggaaat aagagaaaca ataagagtta ttaataaata
    11941 tgttttaaaa actccattac aggataatga acttgaagta attttgagag acgaatcctt
    12001 tcaaaagaag atatttttta attcaaaagg ttcattttta tttgatgagt ttgctaaata
    12061 cataaaaaat aataatcatg taatcaagat aaatgaccag ttacatttat ataaggatgg
    12121 tatctatgta gatggtcaag caagaataga agcagaaatg attaataata taagcaattt
    12181 aaataaggct aagaggagtg aggtgcttag ttatttaaat ttattaataa gtgagaacac
    12241 aagtatgtca gaagctaatt taatcgcttt taagaatggt atatataaca tagttgatga
    12301 ttcttttata gaattttcac cagaatttat tataacaaat aaagttaact ggaactataa
    12361 tccaggagca tattcaaagt tagttgataa aactatgaat aaattatcat gtggagattt
    12421 tgaaattaga atgttacttg aagaagtagt gggatactgt ttttatagac gtaatgagct
    12481 tagaaaagct tttatattaa ctggagataa agcaaatggt aaatctacct atcttgatat
    12541 gataaagaca ttattagggg atgaaaatac atcagcactt gatttaaaag aattaagtga
    12601 tagatttaaa actgctgaat tatttggaaa acttgcaaat ataggagatg atataggaga
    12661 tgaatttatt gctaatcctg ctatatttaa aaagctagta agtggcgata gagtaaatgt
    12721 tgaaagaaaa ggtcagaatc catttgattt taacaactat agtaagtttt tattttcagc
    12781 taacaacata cctagaataa aagataagac tggagcagta cttgatagat taattattat
    12841 tccttttaat gctagttttt cagtaaatga taaggatttt gacccttata taaagtacaa
    12901 attaagagag caagaagcta tagaatattt aataaatcta ggattagaag gcttaaaaag
    12961 agtattgaag aatagaaaat ttacagtacc agataaggtt aaaaaagaaa tacttgaata
    13021 tgaagaagtc aataatccta ttttaggatt ttttaaagaa gttgataaga tagaaaatga
    13081 atcaacaaaa gaaatatata ggaaatatca agagtattgt atattaaatg gattacaacc
    13141 tatatcaaac attgagtttt caagacaagt agtcaaaaag tttggatatg aagtaaaaga
    13201 taaaagaata caaggtaaga aatataaagt atttactaga gcagtcccgg gatagtcccg
    13261 gatgtagtcc ctagatgtaa tatagtaatt tcaacgttgt cccggatgtc cctagatgtt
    13321 tttgaccttt tatatattta actaaaaaat catcaatttt ttgtgatgat tttgctccct
    13381 ttatataaag aatatatata taatagtatg ggactgaaca aatgttattg tctggatata
    13441 ttgaaatagc tagacttgga gcagtcccgg atgtatttta catctgggac tatctgggac
    13501 tgggtttaat taagaagtta tacaatataa aaatatttaa aaaataagct atatttcatt
    13561 aataacttaa caaaataatt aaaaagaaaa ttctgttaat ttgcaaggat gtgaaaatga
    13621 agtgacagac tatgaaaaat tagaagaatt gttaaactca tatagtaatt tggatattga
    13681 aattagagaa atagagttaa aagtaagagg gagtagttta agaggaatag aattaaatgg
    13741 aatgcctaaa ggaaataatg tttcttcacc aatagaaaat gagttaatat atgttgaaag
    13801 attggagaat gagaaaatat atctacaaat taaaaaagaa tcgattaata atatgttaaa
    13861 tttattagat gattttgaga aaaatctaat agaactaagg tattttaaaa aattacaata
    13921 taaacaaata agttatgagt taaatataag cgaattatat gtaggaaaaa agagaaaaaa
    13981 agcactagat aaaatacttc cctttgctaa aaaatataat ctaatatgaa agtcttgttt
    14041 gtgtccgatt tatgaccgat taatgattga tttagtatag aaacatgtca catcaatata
    14101 ctatgagata agatataatg gtagtatgaa acaagtgtat aatatatccc cttgaaaaag
    14161 gctaagttta ccccaaactt agcctttttt atatttaaaa caaagtgagg tggtgatgtg
    14221 gctaaattaa ctgaaaaaca aaagaggttt tgtgattact acattgaaac tggcaatgca
    14281 acagaagcag caataagagc aggatatagt gaaaagactg ctaaagtaat aggtgctgag
    14341 aacttaacaa aaccatacct aaaaagttac attgatgaaa gaataggtca acttgagtca
    14401 aataggatag cagatgcaaa agaagttatg gagtatttaa ctaagatagt aaggaatgaa
    14461 gcaaaagagg aagtggttgt agtttctgaa tatggtccag aaataataaa aaaagacgta
    14521 agtataagag atagaaacaa agctgctgaa ttattaggta agaggtatag gttgttcact
    14581 gataaggtag aagttgaagg gactggtata gttcaaatag tagatgatat agatgaatga
    14641 gattagatta aaaaatatta tagctccaag cttctataat gtacataaac ttattaagca
    14701 agatgtttat actcattatt ggtttaaagg tgggagaggt agtacaaaat catcttttat
    14761 atcattagaa attgttttag gtatgatgag agatgctcaa caaggcaaat taacaaatgc
    14821 agttgtaatt agaagagtta aagacacact tagaggttct gtttatgaac agatacagtg
    14881 ggctatttac acattagggg ttcaaaatga atgggaaata ccagaatcta agcttcaaat
    14941 gacttataaa ccaacaggtc aagtaatttt atttaaagga gctgataaac ctaagaaatt
    15001 aaaatctact aaagtgtcta aaggttatat aaaatatgtt tggtatgaag aagtagatga
    15061 atttgaaggt atggataaaa ttaggaatgt taatcaatct ttgcttagag gtgggaatac
    15121 atataatgtt ttttactcat ttaatccacc agaaagtcaa agaaattgga ctaatatgga
    15181 ggtgatagaa aagagagaag ataaatttat tcatcatagt aattatttat ctgtaccaaa
    15241 agaatggtta ggagaacaat ttatagtgga ggctgaacat cttaaaaaag taaatcctac
    15301 taagtatgag catgattata tgggtgttgt aactggtaca ggtggagagg tatttactaa
    15361 tgtaacggta agagaaatat caaatgaaga gataaataga tttgatagag taagaagagg
    15421 tcttgacttt ggatatgcta aagacccttt atcttatatt gttatgaact atgataaaac
    15481 aagaaaaagg ttatatatat tctttgagct ttataaagta gcattaggaa attcaaaagc
    15541 tgttgagctt attttaaaag aaaacacaag caataaaaaa gtaattgcag atagtgcaga
    15601 acctagaagt ataaatgagt ttaaaaagct aggtttaaaa ataattggtg ctagaaaagg
    15661 tccagatagt gtagaacatg gaatcaaatt cttaagtgag gaagtagaag aaataataat
    15721 agaccctata agatgtccaa atgctaagag agaattttta ggatatgaac ttgaaaaaga
    15781 taaagaaggt aattttaaag atgagtatcc agataaaaat aatcatacta tagatgctgt
    15841 tagatatggt atggaagatg taatgttaaa acgaaaggga ttatcaatac ttaaataaaa
    15901 gtgaggtgaa tagatgaaca tagaagtagt taagaagctg attaaaaaac atgttcccta
    15961 tcattctgat gtaatatcta aatcaattat atctgaaaga tactataaga ataaaaatga
    16021 tatattattt aaaacttcta atagggaaga ggaagaaact gaaaatccac tcagaaatgc
    16081 tgacaataga atatcaagta gtttctatag cttgttagtt aatcaaaagg catcatactt
    16141 atttacttat ccaccaactt ttgatgtatc taataataga acaaataaag ctattacaga
    16201 agttttaggt gatatctatc ccaaaatatg caaagactta tgtataaatg caagtaatag
    16261 cggagtatca tggttacatc tatggaaaga taaaaacagt aagttaaatt atggtgtggt
    16321 tgacagtaaa caagtaatac ctttatggtc ctctgattta aataaaaaac ttataggggt
    16381 acttagagta tatgatgaaa tagatgaaaa tggtgacaac tatataatct atgaatattg
    16441 gacagataca gagtgccaag catatagaaa actagatgac ttagatatag atgaactaga
    16501 agtttataat atgttctcta taacatatgg aaatggagat atattagaca atagcaatac
    16561 acttaaacat gattttggag aattaccatt cattccattc tttaataata atataaagac
    16621 aagtgatttg gatactgtaa aatcgttaat tgatacttac gacaaagttt ttagtggttt
    16681 tgtaaatgat ttagaagata tacaagagat tatatttatt ttaaccaatt atggaggaac
    16741 agatttaaaa agcttcttgg aagatatgaa gaagtataaa actgttgagg tagaaagtaa
    16801 tgggatggat gataaaagcg gtttaagcac acttacaatt gatattccag ttgaagctag
    16861 agaaaagtta cttatgatga caagaaaggc tatatttgag caaggacaag gtgttgaccc
    16921 acaaccaatt gactttggta atgcaagtgg tgtagcactc aaatatttat actcactttt
    16981 ggaacttaaa gcaggtctta tggagactga gtttaaattg agttttggaa aactaataag
    17041 aatgatatgc aaggttttga atttggaatg taatatagta aatcaaacat ggacaagaaa
    17101 tgctattgcc aatgatttag aattagcaga gatatgtgca aaaagcgttg gtattgtttc
    17161 tgataaaaca gtatgtaaaa atagtcctat tgtagaagat gtagagtatg aaatagcaca
    17221 gattaagaag gaaaaagaag aagctcaaag agagtatgat gatttgatac cgaatgatgg
    17281 tgttatagat gaaacataaa gattattgga ggaagagatt tgaacaatta gaagaagctc
    17341 aaaataacaa aagtataaaa tattatcttg aattagaaaa acaatataaa ttagctatgt
    17401 ctagtataga aaaagatata ttagcatggt ataacagatt tgccaaaaat gaaggaatat
    17461 ctttattaga agctaagaaa ctgctgaata caagagaact agaagagttt aagtggagtg
    17521 tcgaagaata tattaaatat ggtaaagaaa atgctataaa tcaaaagtgg atgaaagagt
    17581 tagaaaatgc tagtgcaaga gttcatataa caaggcttga agctttaaag ttacaaatac
    17641 agcaacaagt agaagttctc tatggaaatg aacttgatgg tattgataaa ctaatgagag
    17701 atatttatac aagtggatat tatcatacag cttttaatgt tcaacaagga gtaaacgttg
    17761 gttggagttt aatgagtctt gatactaaca gaataaataa agttatctct aaaccatggg
    17821 caacagatgg attaaacttt agtgaaagaa tttggggtaa acatagacct gctttaatca
    17881 atgagctata tactaaactg acacaatcaa ttattagggg tgagaatcca aagaacctag
    17941 taaatgactt tgctaagaga tttaatgtgt ctaaatctca agctaagaat ttaataatga
    18001 ctgaatcagc tttctttgca tcagcaagta gaaaagattg tttcaatgat ttagatgtag
    18061 agaaatatga gattattgct acattagatt taagaacttc aaatatatgt agagagctag
    18121 atggaaaagt atttgatatg aaagattatc aagttggagt tacagctcca ccatttcatt
    18181 gtcgttgtag gacaacaaca gctccttttt ttagtgatga agaaggctat agagcagcaa
    18241 gaggagaaga tggaaaaaca tattatgtac catctagtat gaagtataaa gaatggtatg
    18301 agaaatatat taataaaaac attaagttat cagataatga acaacttgca attaataaat
    18361 atgtaagtag tgattcttat aaaattaatg agaaacttag aagagggtta tcattaacaa
    18421 gtgaagataa aaattttata agtaacttag atagtgcatt agataagatg cctaattata
    18481 agggaaatgt gaataggtcc ttgtatttct ttaatgaaaa agaaaaaata gactttttaa
    18541 ataaacatca aataggtaag gaaatagtat ataatgaata tatatctaca tccagtagag
    18601 gggaatacaa tccaagcggt caagttgaat taaatattat aagcacaaat ggcaaggata
    18661 taagaaaata taatcctcaa gaagcagaga ttttatttaa aaggaatagt aaatttataa
    18721 ctactgataa ttttgaatat gatggcaaac attatataac aatgaaagag gtgtaatatg
    18781 aaaaaggata atgaaaaatt attttcagca ccaagatgga cagaaatacc acaagctaaa
    18841 gtggtagggg aaagaacatt aacagaagaa gaagtgaaag aagcgcaaga aattagaaaa
    18901 aaaataatta aaagaaagat taataataaa ggatagaagc acttgctaaa tgataaatta
    18961 gtaggtgctt ttattatgta aaaatttatt gagagggtga tttgaaatga ttaaattata
    19021 tattttatca ataattgtat tttgtacagg gctttattta tttaaaatga agattgatag
    19081 taatgaggaa ttgattaaga ttcttaaaaa taaaaaagta agaagaaagt gtaattttat
    19141 ttttatggct ttatttccac tacttaattt tattttaggt gtgatattta tcatatcttc
    19201 tttactagtt agcaatgaag atataattaa aaatttaaag ggggataagt aatatggcta
    19261 aatttgtgaa gaaaccagtt gaagtagaag cttttaaact aggttatgat gtagaaccga
    19321 aatggtttat tgagaatgat agagtttgta attttataaa agaaaaatgc attaatggac
    19381 atgtaagttg tgacttagaa acattagaag gtactatgag agctaataaa ggggattaca
    19441 ttatacaagg tgtaaaagga gaaatatatc catgtaaagc agatatattt gaaatgacat
    19501 atcaaaaagt agaatataga ggaaaaaata aattatcaac agagatgact ttagattcaa
    19561 caaactttca agaaaatatt aaaaatgcca caaaggaatt agagtttttt atagaaactt
    19621 tagaaaaagc agatgataaa attgataaac tagcagaaaa aatgaataaa tgtgattgta
    19681 aagttgatat agataagatt gtaaaacagt taggggaatg cctaagagaa agtattgagt
    19741 agattttggg gggtgaaaat atgtttaaaa aagacacaag aggatatata aaaagacaca
    19801 ttggaaagcc aaataaatca agtgttagag atattatatc agttcttatt ataattgttg
    19861 ggatagcttt aggctttatt gtaggtttaa gtataattat agcaaatgta ttagatgtag
    19921 ctaatatgat agatactaat acttttactt gtatgaagct tgtttttaat ttagcaggca
    19981 tgatagcagg atatttagta ggaagaataa tctgttttgt attatcgcta acaggtattt
    20041 ttattggtag taaataagtt acttaaaagt cttttaaaag gcttttttat tatgtaaaaa
    20101 tgaaaggaga tatttaaaat atggattggt taaaagaatt attagaagga ataaaagtag
    20161 aagataacaa aattgatgta gcttctcttc aaaagtctat agaaaagaaa ataaaagaga
    20221 ctacagttac tcaagaagat tatacaaatc ttgaaacaca gcttaataca gctaatgaaa
    20281 ctattaaaaa gtttgaagga ggtatgacaa aagaagatgt agagaatcta aaagataaac
    20341 ataataatga tttagaaaag ctaaaaaagg atatggaaat ctcaaaaaaa gaatatgaat
    20401 tgaaaggcaa attaaaagat ttaggtgttg tagatgctga ttatataatt tataaacaag
    20461 gaggaactga aaagtttaat tttgataatg aaggaaaagt aattggttta gaagacacaa
    20521 taaaagttta taaggaaaac tctccacatt tatttactgg tggtaaacca aattatattc
    20581 ctaaaggtgg agatgattat aaaggttcta atccttggtt agaaaaaagt tttaatttaa
    20641 cagagcaaag caaaatatat aatgataacc cagctctagc aaaagaatta atgactgctg
    20701 ctggtaaata acataaagga ggtaaatatg aataaaaata aaagtctaaa aatgaatata
    20761 caattattag ctgggaatga aggaggaaca aagctaagtg atataattgt tccaagcttg
    20821 tttaatccat atgtaataaa tagaacaatg gaaaaaagtg cattattaca aagtggaatt
    20881 ataactaata actctgagtt tgataaatta gcttctcaag caagtccact tgtaaatatg
    20941 ccattctttg aggatttaac aggtgaatca gaacaaataa ttgaagatgt tgatttgcaa
    21001 aataataaaa taacttcaaa tgcagatgtc gcagtaatta tgagaagagc aaaaatgtgg
    21061 agtgctacag atttgagtgc tgctttagca ggaaaagacc cagcaggtgc tattgctgaa
    21121 ttagtatctg gtttctgggc tagagatatg caaaaagaac taattgctat tcttaaagga
    21181 gtatttggaa ctactaaggc agttggagaa acagtagcag aaactaggtt agcatcaaat
    21241 atactagata tttcttcaag ttctggtaat gctgcaaaat ggagtggttc agcgtttatt
    21301 gatgcacaac aaaagttagg tgatgcaaaa gaaactttaa cagcagttgc agtacacagt
    21361 gctgttgaag ctgaactaag aaaacaaaat ttaattgaaa ctgttcaacc ttctaatagt
    21421 ggtccaatat atttttatca aaataaaagg gttataattg atgatggttg tccttatgaa
    21481 ggaagcggtt ctaatatagt atttacttcg tatttatttg gtgaaggtgc aatagcttta
    21541 ggaaatggta atccagtagg atttgtagct acagaaatag acagagataa gaaaaaaggt
    21601 tctggtgttg attatattat aaatagaaag acttttatca tgcaccctag aggagttaag
    21661 tttacaaatg aaaatatggc taaaacagaa ggtccttcaa gagctgaact atcagatgca
    21721 actaattgga aacctgttta tgaacctaaa caaattagaa tggtagcttt tagacataag
    21781 atatagatat ggaatataaa ataataaaaa ttaaaagact cttagggtta aatgagagtg
    21841 atagttctaa agatacaata ctagagttta tactagaaga tgtagaagaa atagtcaaaa
    21901 actattgtaa tgtacctact ataccagaac aattaaatag tactatttta agaatggcta
    21961 tagaaatgta taaaaatgag agtctaggaa gcgaagatgt tgcactaggc tctatttctt
    22021 ctatatcaga aggtgacaca tctatttcat atagaagttc agctagtgaa tttaaggaat
    22081 ctttacttaa agattataaa gcacaactaa atagatacag aaaaattagg tggaaataat
    22141 gatggataag actagaaaag caatagaaat gttatataga gataaatgta ctatagttga
    22201 gtatcagcca atcaaagacc ctgtaacaaa acgaactaac aataaagaag tgattgtatt
    22261 agaaaatcaa ccatgtaagc tttcatataa aaatattaca tcaactacag atgaaaaagt
    22321 agctaaactt gtacagacta ttaaactctt tatatctcca aacataaaca ttaaagcagg
    22381 ttcaaaactt attataaatg ataaagaata tgtaagaagt ggagaatcag ctatatatcc
    22441 aaaccatcaa gaaattatct tagaattact taaggacaag gcataatggc tagatggggc
    22501 agtgttgatt ttagagagtt taaaagagct tgtagaagaa tggagaaatt tacaaagatt
    22561 gatttagata agttttgcaa agatgcagca agagaattag cagcaagact gcttggtaaa
    22621 gtaattagaa gaacaccagt tgatacagga tttctaagag aaggttggag tggaatagct
    22681 tatgctaggt cacttccagt gtataaaagt ggtaataatt acatcataga ggttatcaat
    22741 ccaacagaat atgcttcata tgttgagttt gggcatagga ctaagaatgg taagggatgg
    22801 gttaaaggac aacatttttt aactatttca gaaatggagt tacaaggtca gattgataag
    22861 attatagaga aaaagctatt aatattgctt aaaggagtgt ttgatgctta ataatattat
    22921 agatggaata tctattaaat tagataaatc gtttggaaat gaatatacaa tttatagcga
    22981 agatgtggag caaggtataa atgaatcttg tttttttatt gttcctttaa atccaagcaa
    23041 agtatcatat ccaagtggca gagaattaaa gaaaaattct tttgatgtac attattttcc
    23101 taaaagtaat gataaatcat ttgaaataaa tgaggtagct gagatgctac tggaggaatt
    23161 agagtatata gaaattgatg gagatttagt cagaggtaca aacatgaact ttgaaattgt
    23221 agataatgtt cttcatttct ttgttgatta taactacttt actataaaaa gtaatgatac
    23281 agataagatg gatacagtag agttattcgg tggtttgaag agaggtgata attttgagta
    23341 aaacattgaa taaagaagat gactataagt ttactaaaga gcagatagtt aattctaaaa
    23401 agtatgtaaa tagaaaagac ttattaaatg caattttaaa agaaaatgag ttatattctt
    23461 tctcagaggt agagaaaata ataaataatt ttatgaaagg agtgagttag atggctttag
    23521 gaggaggaac atttgtaaca cagaataaag tattaccagg tagctatata aattttatct
    23581 cagctaaaag ggcaaccagt tcattatcgg atagaggtat tgttgcaatg cctttagagt
    23641 tagattgggg catagatgaa gaagtatttc aagtcacaag tgatgatttt gagaagtatt
    23701 cagtgaagta ttttggatat gattatactc atgagaagct gaaaggcttg agagatttat
    23761 tcaaaaatat aaggttggga tatttttata aattaaataa aggcgttaaa gccagttgta
    23821 ctatagctat agctaagtat agtggaataa gaggtaatga cttaaagata atagttacaa
    23881 caaatataga tgataatgct aagtttgatg ttgtaacact tttagataat aagaaggtag
    23941 atattcaagt agctaaagtc attacagact tacaagacaa tgactatatc acttggaaga
    24001 aggatgcaac attagaagct acagcaggat taacttttac aaatggaact aatggagaag
    24061 cagttacagg agcagaatat caagctttct tagataaaat agaaagttat tcatttaatg
    24121 ctttaggatg tttggctaca acaacagaga ttaaaagttt atttgtagaa tttacaaaga
    24181 gaatgagaga taaggtagga gcaaagtttc aaacagtact atataagaaa agtgatgcag
    24241 attatgaagg tgtagtgtct gtagaaaata agattaaaga taaagattta gttgaatcta
    24301 gcttgattta ttgggctact ggagctatag caggatgcga tataaataaa tctaatacta
    24361 ataaaaagta tgatggtgag tttgatgttg gtgtaaatta cactcaaata caacttgaag
    24421 aagcactaaa gagtggtaaa tttatatttc ataaagttgg tgatgaagtt catgtgttag
    24481 aggacataaa tacttttgta tcatttacag atgataaaaa tgacgatttt tcaagtaacc
    24541 aaagtgttag agtacttgac caaattgcta atgatatagc gactttattt aatacaaagt
    24601 acttgggtga agtaccaaat gataaatctg gtcgtatctc gttttggaat gatgtagtta
    24661 agcatcatga acaactgcaa aatatgagag caatagaaga tttcaaagct gatgatgttt
    24721 ctgtagaacc tggaagcgac aagaagactg ttgtagtaag tgatgctgta aaagttatta
    24781 gtgctatgag taagctttat atgactgttt cagttagtta acaataagaa aggagaataa
    24841 taatggcaca acaaataaaa gcaagagata caataagtgc atctaaggca gagtgttttg
    24901 taactataaa aggtaaaaga tataatttta tgcaagctat taacttagaa gctaaaatgg
    24961 aaaagaataa gagtgagata cctatattag gtagtactac aaaaggaaat aaatcaacag
    25021 gaagtaaata ttcaggaaat gcaacatttt attataatac ctctatattt agagaattgt
    25081 tgtatgagta taaagaaact ggtgaggata tttacttcga tatacaaatt accaatgaag
    25141 acccaacaag ttcagtgggt cgtcaaacta taatactgga agattgcaat atggactcag
    25201 gcataattgc taaatttgat gctgatgggg agtatttaga tgaagatatg gattttactt
    25261 ttgagaattg gaaattagtt gagaaattta atatagcaaa tggtatggag taaaatacac
    25321 atttatgatt tatgtatgtg tattttttat ttataagaat aggagatgat taaaattaag
    25381 gataaatatg agataaaaga ttcaatttct tttgattata gcaataaaag acctttggaa
    25441 gaacgtgtca gcgagatgta taaaaaggca ggaaaatatc ttatagatat ttcagataag
    25501 ttagcaacag atacaattga tggttcgtca ttaaagccaa taatcataaa atttgaaata
    25561 aatgaagctg gtgttgcaac aatagaaaaa caaacaaaat atttggtgat ggaggtagaa
    25621 taagaatatg ggagatttaa acgctttttt aagtcaaaat gcaataaaag tagagaatag
    25681 aaagtatgtg gcaagtgaaa ggtttatagg agaagatgga aaagcaatcg aatgggaact
    25741 taaagcaata gattcagata gggacagaca attaagaaaa gattcaacta taagagtacc
    25801 tatattaaat aaaaaaggga aagctacagg acaatacact agtgaaacag attttaatac
    25861 ttacactttg aaattatgtg tggaaactat agtatttccg gatttacatg atgcagaatt
    25921 acaaaatagt tatggtgtaa tgggtgcaga ggagttatta acaacaatgt tgactcctgg
    25981 cgaatataca gacctttcaa gtgaggtagg agaggtaaat ggttttgata gaacttttga
    26041 agataaagta gaagaagcaa aaaactaatt tatgaaggtg attatgattc taatatagct
    26101 cattattgcc ttcataagtt taaatggaaa ccgcatgaat atatggattt accagttgat
    26161 gaaaaagcat ttgttgctgc ttctatagat atcaaagtag aagaagaaaa agaagaagct
    26221 aaaaaagctg acaaagatgc aaagagaggt agaagaagat aatctctggt gtaaaaattt
    26281 tactaatata gtataatata tttaaaaatt gtactggggg attaaataaa tgggattgtt
    26341 tagtaaaaag aataagaaac cttgttgtat ttgcggaagc gaaaaaggac ttatgccatc
    26401 tatagaaggt gaaaattttt gtacagcttg taattgcaag tacatagatt tctctgaaaa
    26461 tattttaaaa gtaactagta ttatgaaaat gatgtcaaac tcagaaggaa tgaaaaagtt
    26521 tatagaagtt gaaaagaaaa atttaaaatt gttagagaag ttcaccgaga caaaaagtat
    26581 aaattcttca atttcttttg atgaagagca aaatctttta aaaatatcat ataaaaatag
    26641 aaatcaaata ttagtagaga aaataataaa atttgatgat atattagaat ttgaactttt
    26701 ggaagatgga gaaactatag ttaaaggtgg tttaggtcgt gctataactg gaggagtctt
    26761 attcggaggt acaggggctg tagttggagg aattacaggt aaaaaaacaa gtagaaaagt
    26821 tgtagaaata tttaaaataa aaataactgt aaaggacata aataatccaa ttgaatatat
    26881 aaatttaatt aatagtaaag taaaaacaaa ctcttctatt tatcagaaag ctttttctga
    26941 tgctcaagaa atattatcaa tactatcaat aattacaaaa tataatgtaa tggaagacaa
    27001 aaaaaagtct atatctagtt ctacagctga tgaaatatta aaatataaga atttgttaga
    27061 tatggaagct attacacaag aagaatttga tgctaagaaa aaagaattgc taaatttata
    27121 atatataagc acttacttaa aaataagtaa gtgcttatat tatgttaaaa aagaaaggag
    27181 gttaaaaatg gcaactatac aaacatctat ccgaattttc gacggaatga cacctgcttt
    27241 tagacacatg aataatgcta tgaatattgt attaagttca ttcgagcaat tacaaagaac
    27301 atctagcaat gctatagatg ctaatagtat tagaacagct agagaagaac tagcacgtgc
    27361 agaagctggc tttgatagac tagaacaaca aataagagaa tcagataatc aacagcgaag
    27421 gcttaatgag gatataaata agggtgcaag ttctacagat agattagttg gaagtgcaaa
    27481 gaagctagca gcaacctact taggtataag aacgttagga ggtctaggaa atttaagcga
    27541 ccagatgaca agtactaacg cgagacttag tatgataaat gatggtcaac tctcagatgg
    27601 aggattaaat aagatgattt tccaatctgc tgaaaggtct cgtgcatctt acttagatac
    27661 tgcaaaaata gtttcacgaa taggcatgaa cgcaggtaag gcgtttagca gtacaaaaga
    27721 aattgtaggt tttgcagagc aactaaataa aaagttcgta attgctggag caagtactga
    27781 ggaaatgaat tcggcattgt tacagctaac ccaagggttg agttctggtg tattaagagg
    27841 tgaggaactg aatgctgtgt ttgagtcagc acctaacatc atccaatcga ttgcagatta
    27901 tttggacgtg gacataggaa aaataagagg aatggcatca gagggaatgt taacagcaga
    27961 tattgtaaaa aactcattac ttgcagcagc agagcagact aatgcagagt tcgagaagat
    28021 gccttatacg ctaggtcaaa tctttactag tgtaaaaaat aatgcagtta tggtatttgg
    28081 agctatacag aagaaaattg aggacacagt ttcaagcagg ggatttcgaa ctttcataac
    28141 tgatgttaaa gactcattat atgtacttgg agcagttggg tttaatgtat ttagtggatt
    28201 tattaattta ctgagtagtc cagcttttca gaattttttt aatgtgatga ttgttggaac
    28261 tagtttagtt gtacaagggc taggttggat aataacacaa gcacttagtg ttgctaatgt
    28321 attttcgcga aattggagta taatttcacc tataatttta ggtgtagttt cagctattag
    28381 tacctatata atagctcttg gtattatgca agcagttact atagctggaa ctgtagcaaa
    28441 ttggatgtcg tggacatctg aagttattag acaaagagga attttaggaa cagctagagc
    28501 tttagcgata ggcaaagctg aacaatgggg atttaatgct gctatatatg catgtcccat
    28561 tttttggata gttttaggta taattgcagt aatatctgct gtatttatat tagtagcagt
    28621 ttacaatcac tttgcaggca caagtatttc tgcaatcagt cttatagttg gagcttggta
    28681 ttggttatgt gcagttgttt acaacgtaat tgtagggata gtaaatgcta taaatatctg
    28741 tgtggttgaa atagcaaata tctttagaac tggattatat gcagtgcaat gtttctttat
    28801 tgatatggct aatgcaggac tcaaggcagg tgttaattta gacaaagctt ttgataaatt
    28861 tgctaccaat ctagcaaatg gtattataaa agctgttaat atagctgtaa aaggattgaa
    28921 ttggttagta caacagatta ataaaattcc aggaattgat ttaccacaaa tgaaagaatt
    28981 tcaaaaagta aatactgtaa ttggtacaaa aacaacattt aaacccattc aaaaaccgcc
    29041 tgagcctaaa gcatggaaac cagaattagt agagtacaaa aatttaaagt cagaatttat
    29101 aaaaggatat gacgtaggac atcaattaca aaacaaattt aaagatactt ttgacattag
    29161 caagatagcc gaaaaagcaa aaaaagattt aggtctagat gacctttggg ataaaaaata
    29221 cggacttggt gatggattgg gttcagctgg attaaattca cctttgagtg acgcagcaaa
    29281 aggagcaaag gacacagcag gcaatactgc aaaaatggca aagactatgg ataaaagtca
    29341 agaggactta aaatatctta gagacattgc agagcaagaa acaataaacc gattcacagg
    29401 ggtaaatata aaaattgaca tgaacaacac taataacata agtaaagatg cagatgtgga
    29461 tggtatagtt aatgtactaa cagaaaaact gaatgatgct atggttgtat ctgctgaggg
    29521 aatagtttag agaggaggtg taatcgtggc ttatgatttt tatttagatg gagtacaatt
    29581 accaatacct ccaccaaagt tagagattaa agttacaaat aaaaataaga cagttgattt
    29641 gataaatact ggagaagtaa acatattaaa aaaagaaggg ctatctgaaa taagttttga
    29701 agcagagttt acacataata aattaccttt ttatcgtgga acttttaggg atgttcaatt
    29761 ctttttaagt aagctagaat tactaaaaac tgattgtaag ccatttcaat ttattgtatc
    29821 gagggaaatg ggtggaaaag tactatttaa cactaatatg aaagtgtcat tagaagaata
    29881 taacatagta gaagatgcag acaatggttc agatgttaaa gttgcaataa agttaaagca
    29941 atatagagat tactcaacta aaaaattagt tcctgcaact cctgaaaaga caaactatgg
    30001 taggactccc actccagtca tgaaaccaaa agaatttaga ccagattcat ccaataagcc
    30061 aaatggtaaa acatatacag taaaagcagg ggatagcctt tggtcaatct gccagaagca
    30121 acttggtaat ggttcattat ataagaaagt atatgagtta aataaaacaa tgatggataa
    30181 agctaacaag ggtaaaaaag tacctaaata cacaatttac aaagggcagg tgttaaaact
    30241 tggctgatga attagttctg gcaaatgata gggatgtaag gctagttatt gcacattggg
    30301 aagatttcta cgaaccagtt gtccttgatg gtatcacatg ggagatagaa agaagaggaa
    30361 caccttctaa gttagaattt acaatagtta tggatgatat attagagttt tgcgaaggaa
    30421 attctgtaag gctgtattat aaaggaatag gtatcttcta tggatatata tttcagaaga
    30481 aaagagataa agaaaatcac attaaaattg ttgcttacga ccagctaaga tattttaaga
    30541 ataaagatac ttatgtatat agtaataaaa ctgcaagtga acttgtaaaa atgttggcta
    30601 aagattttaa tttaaaatac aatgtcatag aagatactaa gtataaaata tctaggatag
    30661 aagaaaataa aacactcttt gacatgatac taacagcact agatgatact ctaagagaga
    30721 aaaaggaaat gtatgtttta tatgatgagt ttggaagaat aacattaaag aatgttgcat
    30781 caatgaaact tgatacggtt atgaacaatg atgtaattga ggattttgac tataattcat
    30841 caatagatag tgatacttac acaaagatta aacttgtcag agacaacgag gagtcaggaa
    30901 aaagagatgt atatattgca caagattcta cacatatgag aagttgggga atactacaac
    30961 tatttgatac agtagataaa aacatgagtg aagcagagat aaagcaaaaa tgtgatatac
    31021 ttctaaaact atataataag aaaactaagt cattaagttt aaaaaatgtg ttaggagata
    31081 ttagagtaag agcaggttgt ttagtacctg tttttttaga tttaggagat attgatttac
    31141 aaaattatat gttagttgag aaagtaaagc acacatttga aaataattcg cactttatgg
    31201 atttgactct tgttgatgga gatgaatttg cttcttattc ttcaagctca tatagtagtg
    31261 gaaatactaa taataaagat gaaaagaaaa atggtcctgc acaaagtact acaagtaaag
    31321 aagacaatga tatgataaat aaattaaata aagtatttaa aaataagtta tcaaatacag
    31381 gaagtatatt tgttaaatat tctaatgctt acaaagtcaa tgcagcttta atggctgcta
    31441 tttctataca tgaaactggt aatggaagtt cttcactttg taaaaataaa aataatttct
    31501 ttggaatgaa aggaatgtct tttggctctg tggatgaagg aataaaaaga ggtataagta
    31561 atttatcaag aaactatatc catatgggaa gaaaaacatt agaaagtatt agagacaaat
    31621 atgctccact ttatgatagt cctcttaata aacattgggt accaggagta aataagtttt
    31681 ataaacaaat aacgggaaat gcatatagtt ctaatagtgc aggtacaggt gttagaagca
    31741 acgaggaagc agaaaagaat ttaaaagata taacttatca agttcaaaat agcaacaatg
    31801 ctaatacatc aacaaacaat aatagtaaag taaataaagt tattcaagaa gcaaaaaatc
    31861 aacttggcaa gccttacaaa tggggtggta atggtccaaa gagttttgac tgtagtggtc
    31921 ttatggtgtg ggcattcaaa agaggtgcag gtataaatct caaaagagtt tcagcagacc
    31981 aatcaaatga tagtagagga aaactattat gcaacataaa tgatgtaaaa gctggcgatt
    32041 tagtattctt tgcatacaac caaggaaaag gaaatgtaca tcacgttgga ctatgtatag
    32101 gaaatgacaa gttcatacat tcaccaaata ctggtgatgt agttaaaata agcagtttaa
    32161 gtggtagaca aaaacaaaaa catgattttg caagagctag aagattcttt taagtgaggt
    32221 gataatatga gccaagattt attacaaata ataaagaaag ctgcaatgga tgcagtagaa
    32281 acaagcaacc caatgcagat tgcatttgga actatagaaa gtgttaatcc tttagtagtt
    32341 aagatagagc aaaaagcctc ttttgaagaa tcttttctaa tacaaacaga tacatttaaa
    32401 agatatacag ataaaaagat aggagataaa ttagtcttaa ttaggatgca aggaggacag
    32461 caatatttga ttttagatag gatgtgatga aatgttacca acagataaca ttgactatga
    32521 tatagaagat gtatcgataa ttaattttga tgtaaggcaa gaaccaagta agacctttaa
    32581 attaaatata gaaaaatcta agatagatgg tatttgtgat gatgtggaag cattaaaaca
    32641 aaccatcttt ttaattttaa acacagagag ataccaacat ctaatatata gttggaatta
    32701 tggagtcgag ttgaacgacc ttattggaga gcctatatcc tttgtaatcc ccgaacttga
    32761 aagacgaatc aaagaagcac taattcaaga tgataggatt gaaaatgtag ataattttga
    32821 gtttgaaaat ataaaaggaa aggtcaattg taaatttatg gtccatacca aatatggaaa
    32881 tatcaaagca gagagggtgg tgagtgttta gtgtttgaat ttatgacatt tgaaaatata
    32941 attaaaagaa tgctagatag tgtacctagc acgcttgaca agagagaagg ttctataata
    33001 tataatgctc ttgctcctgt tgctatagag ttatctgaaa cttatattgc tatggatgaa
    33061 ttactagacc aaacattcgt agatactgcc agttattact acttggagaa acgatgtaag
    33121 gaaagaggaa ttacaccact cgaagcaact aatacaattg caaaaggagt ttttaacata
    33181 gatattccac ttgattctag gtttaatcta ggagaatata actatgtagc aattgagaga
    33241 atatctgaat gtatatataa gatgaaatgt gagactgcgg gacctatttt tgagttgggg
    33301 caactaatac ctatcgaata tatagacaaa ttagaaacag cagaactgac agaaatactg
    33361 ataaatggag aagatgaaga atcagaggac agtctaaggc aacgatatta tgatagttta
    33421 aattcacaat catttggtgg aaatatgcaa aattataaag atgaagttaa caaaatacaa
    33481 gatgttggag gagttaaagt atatccagtt tggaacggtg gaggtactgt taagttagta
    33541 ataattaatt ctaacttcaa agttccatct agtgatttag ttaatttagt tcaagaagaa
    33601 attgaccctc tacaaaacca aggagaaggc cttggtttag cgccaatagg tcatagagtc
    33661 accgttgaag gagttacaag tacaactata aatatatcag cagagataac atacaagagt
    33721 ggttacactt gggagaatat aaaattaatt gcagaagaag taattgatga ctatttaaat
    33781 gaacttaaca tgagttggga agatgaagaa aatttaatag tccgtatatc tcagatagaa
    33841 actcgtttgt taagtataga tggagtgtta gatattacaa acacaatgac aaatgatgtt
    33901 aaatctaatc taacaataga tagtaacagc atagtagtga gaggtgaggt agttggataa
    33961 agagattaat ctaataaatt acttgccaca agttttgcag gataaagaag aatatataaa
    34021 agtatttaat gcagaaaaca aagaaataaa aacactacat gataaattaa aggacctatc
    34081 aaatgaccag tttttagagg acctaactat aagtggtata aaaagatggg aaaaaataat
    34141 gtctataaat cccaagtcaa atgagacttt agaggataga aggtttagga tttttagtaa
    34201 atatataagt aaattacctt attcagagag atttcttagg aactggttag atagtatagt
    34261 tggagaaggc aattatgaat taactataaa taattctacc tataacatac atcttgaaag
    34321 tgatgctaga aatcaagatt ggtttgagga agtacattct tttgtaagta gcatcaaacc
    34381 ttgcaacatg agtcttgatt acactagagt gttagttagt aaagataatt acatgaattt
    34441 tggaataaca accctaatgg gtcaagaaat aactatatac ccttggagtc cgccagatat
    34501 agaaacttat ggagaaattg atatactaac aggaaatgga gttggatatc aagaggtaac
    34561 aatattttag gaggtgatat attggctata gataaaagtt attacactat aattacagat
    34621 gtaggaaaag caaagatagc aaatgcaagt gttacaggca ataaagtcgg atttgtaaaa
    34681 attcaacttg gtgatggtgg aggtagtgaa tatactccaa cagaaagcca aacagctcta
    34741 aaaaatgtag tttgggaagg taatattggg aatacaacta cagatgaaac agcaccaaat
    34801 tgtataatac tagagagtct tataccaagt aatgtaggcg gatttatgat aagagaaata
    34861 gggtatttag atgatgaaaa taatctaatt gccatttcta aatataaaga atgttataaa
    34921 ccttctatag aacaaggggc agtagtagac atgaaggtta aaactgtgct gattgtatct
    34981 aatgtaaata atatagaact taaaattgac ccaacaataa tctttgcaac actcaaagat
    35041 atacaagact tagaaactaa aataggtact gttaatacta aaattgatac aaccaaaata
    35101 gaattaacaa gcaacataga aactgctaaa acagagttaa acactagaat tgacacagaa
    35161 aatgagaaac agaatattaa aattgataat attattgcag gtggtgtaaa tgtatctcat
    35221 acacatatta tagaggtggc tgattggact ttaaataatg aaactaatat gtatgaagta
    35281 actataaatc atccactatt gactaaaaga atactaatag ctttatatga tgaaattggt
    35341 gaagcactta caccaaacgc tagggctatt gatgataata gtattcttgt tagaaatgaa
    35401 gaaaatatta aaatgtacgt atatttgata aatggaaatg cagaaactca ttttataaat
    35461 gcaactgtag atgacaacag agtatctgaa atgaccactt attcgtctaa gaaaattgaa
    35521 gatagatttc ttaatttaga agaaaaagta aatggtggga tatctagtat tgcaacaagt
    35581 gtaaatgagt tgataactta ttgttaaagg agagtgagaa aatgcagaca gaatggaact
    35641 ttgggtacaa tggttcgcca caaagtgtta tattgaaacc tggcaaatat aaatttgaat
    35701 gctggggttc ttctggaggt atcaacaatt cttcttggta tactgatgct aaaggcggat
    35761 attctaaagg tgaaattaca ttaaaaaaac aaactacatt atatgtttac gtcggcgaaa
    35821 gtggttttgc ttcttcatct acgagtaata acactaaaag tggttttaat ggcggtggta
    35881 aaggttattt aaatcaacag attatgggta cttattattc tatgtacggt ggtggtgcta
    35941 ccgatataag acttgttggt ggtgcttggg ataatgagca aggtttgcta tctcgtataa
    36001 ttgtcgcagg tggtggcgga ggttcatatt atccttatac tggtggtgca ggaggaggat
    36061 tagcaggagg tactgggtat agtgctaacg acagacatcg tcccggcggt actcaatatc
    36121 aaggtggtat tggtcgtgta agcacagaaa acggaagttt tggaaaaggg tgttctgcta
    36181 aagattcaac tggcgaaggc ggtggaggtg gctggtttgg tggtgcagga atgaatggtg
    36241 cgggagcagg tggaggtgga agtggctacg tattaactaa agatagttat aagcctactg
    36301 gctacacacc aacatctgaa tattattttg ataatgttgt tatggaatct ggtggaaata
    36361 ctgctggtgc ttatggttat gctaaaataa cattactaca agcattacca tttttaacag
    36421 tatcttctta taattccatt acagctacat ttaaagctga ccacacagac cctacattgc
    36481 ttacaaaaat agaatatttt atagatgatg tgttaaaaga aactataaca acagatttaa
    36541 caacagagaa aacaattaac tatacactag aagataatgc actacacaca cttaagatag
    36601 ttgttacaga cagtaataat gctacagcag aaaaagtgtt aagtataagt aagaatataa
    36661 tgccattgcc cgaagatgtg aatctgcaag acatatcttc taaactaatt gaagttaatg
    36721 caggatttaa aactggaaaa acaagtatta taaacacttt agcattaaaa aatatagaag
    36781 caaatttaaa taatacatta gttgaattat cagagaaaat aaaaacaggt tttgatagtt
    36841 cagatgctac tgctgaacaa cttcaaaatc agattactaa tttaaataat cagttaagcc
    36901 aacgtaaaag atgggctaag ggtacttaca cttttactca aagtgattat gaaaattttt
    36961 attcaaggta ttcaagtgct gaaaattatg ctaagagtct tactgttcct attaatttaa
    37021 atttcatccc tagtcttgtc attgttgaaa aactttgttt ttatggaaat gggtatactg
    37081 ttaattttta tgctattaat aatttaaatc aacttgcttc tgttggtcgt gttcctaaga
    37141 gtagtggaga atatcgtttt tgtgctttta tgtttgtaaa aactattact agcaaagaat
    37201 tttctttata tacttatcga gatgaataca ctgatagtag tagcgtagta taccgttgta
    37261 aagtcggaga aagttttaca tggtatgctt atgaataata tgaaaggtgg taatttaaat
    37321 ggaaagagga aatagaatta tatgtgacca aaccgggaaa atattactcc aaactggaga
    37381 agcaacaggg gatatattag aacatgatga aataacggaa ttacattttg ttgatattcc
    37441 gtatggaagt atagactata caagaaatag aattataggt ataaatatag aaacaaaaga
    37501 accaatttta gaggaaatac caacatttat ttcagatgaa gaaaagagaa tacaagagtt
    37561 agaaaatcaa attttattaa atgaaaatga gaaagtagga ggaattttat aatgaatata
    37621 aataatgttg tagtaagaat attagcagag agaatattaa ataagggatt aaatcctcta
    37681 aaaaatagag tatttgaact tgatgatgtt actaacacag agtacaggat ggcagttgaa
    37741 aactacatta tagaacatag tgaggtagta gaaggagcag aacttacaaa ataggtaatg
    37801 ttcttttttt aatacaaagc aataggaggt tttcatgaat gaagaacttg tgaaagaaaa
    37861 aataagtaca cttgaaacaa gagtaaaaga acacggcaaa caaattgata gaatcgagat
    37921 tgagcaagca aagtttgcta tacaaataca aaacctatgt agtgatataa aaaatctgac
    37981 aggagtactc aagtggcttg taggagttat aattacaact ttagtagggt tctttatatt
    38041 tgccatacag aaaggaatat tttaattaat taggaggtta agatatggat aatttaataa
    38101 gttttatacc agagcaactg cttttattag tagttgctct taatgtgtta ggatttggat
    38161 ttaagaaata caaacagtta gataataaat acattccaat catattgctt gtgataggaa
    38221 ttggattttc aatatggatg ttaggattta atccttcatc aattttacaa ggaattttat
    38281 gttggggagt tgcaataggg gcaaatcaag tttacaaaca gttgaaggat ggtgataaat
    38341 aatgaaaata gcaatagtac caggacacac tttaagtgga aaaggaacag gagcaactgg
    38401 ctatatagac gaaggaaaag aaaatagaat tttaactgat ttaattgtta aatggttgaa
    38461 acaaggtgga gctattgtgt atactggaaa agtagataaa tctaataact acttagcaga
    38521 acaatgtaaa attgcaaata ggcaaaatgt agacttagca gtacaaatcc atttcaacgc
    38581 aaataaaaca actctaaatc ctatgggtac agagacaata tacaaaacta acaatggtaa
    38641 ggtatatgcc gaaagagtta attcaaaatt atcaactgta tttaaaaata ggggtgcaaa
    38701 atcagatgtg aggggtcttt attggcttag tcatacaaaa gcaccagcaa tattaattga
    38761 agtatgtttc gtagatagta aagcagatac agattattat attagacata aagacatagt
    38821 tgctaagtta atagcagaag gtattttaaa taagacaata gataataaag agaatggtga
    38881 gggaaaaatc atgtacaaac atacaatcgt ttatgatgga gaagttgaca aaatccctgc
    38941 aactgtagtt ggttggggct ataatgatgg aaaaatactg atatgtgata taaaagatta
    39001 cgtaccaggt cagacagaaa atttatatgt cgttggtggt ggagcatgtg aaaagataag
    39061 ttctatgact aaagaaagat a
//
LOCUS       DAYAOX010000013        37232 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.013, whole genome shotgun sequence.
ACCESSION   DAYAOX010000013 DAYAOX010000000
VERSION     DAYAOX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.013"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(130..324)
                     /locus_tag="ACGI57_000545"
     CDS             complement(130..324)
                     /locus_tag="ACGI57_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715633.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(633..2162)
                     /gene="lysS"
                     /locus_tag="ACGI57_000546"
     CDS             complement(633..2162)
                     /gene="lysS"
                     /locus_tag="ACGI57_000546"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFG9715634.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2177..2656)
                     /gene="greA"
                     /locus_tag="ACGI57_000547"
     CDS             complement(2177..2656)
                     /gene="greA"
                     /locus_tag="ACGI57_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFG9715635.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="ACGI57_000548"
     CDS             complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="ACGI57_000548"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFG9715636.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRATEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3870..4640)
                     /locus_tag="ACGI57_000549"
     CDS             complement(3870..4640)
                     /locus_tag="ACGI57_000549"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HFG9715637.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4659..5249)
                     /locus_tag="ACGI57_000550"
     CDS             complement(4659..5249)
                     /locus_tag="ACGI57_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFG9715638.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTATSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5432..6400)
                     /locus_tag="ACGI57_000551"
     CDS             complement(5432..6400)
                     /locus_tag="ACGI57_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HFG9715639.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6393..7370)
                     /locus_tag="ACGI57_000552"
     CDS             complement(6393..7370)
                     /locus_tag="ACGI57_000552"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFG9715640.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="ACGI57_000553"
     CDS             complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="ACGI57_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFG9715641.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIIVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="ACGI57_000554"
     CDS             complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="ACGI57_000554"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFG9715642.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDGEGIICVGDYKISENYKIDES
                     TKEVLKINFNKL"
     gene            complement(11260..11688)
                     /locus_tag="ACGI57_000555"
     CDS             complement(11260..11688)
                     /locus_tag="ACGI57_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFG9715643.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11705..12511)
                     /gene="murI"
                     /locus_tag="ACGI57_000556"
     CDS             complement(11705..12511)
                     /gene="murI"
                     /locus_tag="ACGI57_000556"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFG9715644.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12713..13198)
                     /locus_tag="ACGI57_000557"
     CDS             complement(12713..13198)
                     /locus_tag="ACGI57_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HFG9715645.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="ACGI57_000558"
     CDS             complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="ACGI57_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HFG9715646.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14053..14733)
                     /locus_tag="ACGI57_000559"
     CDS             complement(14053..14733)
                     /locus_tag="ACGI57_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9715647.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14717..15607)
                     /gene="ispE"
                     /locus_tag="ACGI57_000560"
     CDS             complement(14717..15607)
                     /gene="ispE"
                     /locus_tag="ACGI57_000560"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFG9715648.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15725..17275)
                     /locus_tag="ACGI57_000561"
     CDS             complement(15725..17275)
                     /locus_tag="ACGI57_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOCS domain-containing protein"
                     /protein_id="HFG9715649.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVECIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17369..17635)
                     /locus_tag="ACGI57_000562"
     CDS             complement(17369..17635)
                     /locus_tag="ACGI57_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFG9715650.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="ACGI57_000563"
     CDS             complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="ACGI57_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HFG9715651.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18727..19665)
                     /locus_tag="ACGI57_000564"
     CDS             complement(18727..19665)
                     /locus_tag="ACGI57_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HFG9715652.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19775..19936)
                     /locus_tag="ACGI57_000565"
     CDS             complement(19775..19936)
                     /locus_tag="ACGI57_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715653.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19946..20443)
                     /locus_tag="ACGI57_000566"
     CDS             complement(19946..20443)
                     /locus_tag="ACGI57_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HFG9715654.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20558..21268)
                     /locus_tag="ACGI57_000567"
     CDS             complement(20558..21268)
                     /locus_tag="ACGI57_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HFG9715655.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21444..21974)
                     /locus_tag="ACGI57_000568"
     CDS             complement(21444..21974)
                     /locus_tag="ACGI57_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715656.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21991..22221)
                     /locus_tag="ACGI57_000569"
     CDS             complement(21991..22221)
                     /locus_tag="ACGI57_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715657.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22236..23630)
                     /locus_tag="ACGI57_000570"
     CDS             complement(22236..23630)
                     /locus_tag="ACGI57_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFG9715658.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23756..25009)
                     /locus_tag="ACGI57_000571"
     CDS             complement(23756..25009)
                     /locus_tag="ACGI57_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFG9715659.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTIPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25060..26214)
                     /locus_tag="ACGI57_000572"
     CDS             complement(25060..26214)
                     /locus_tag="ACGI57_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9715660.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGISGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(26774..27343)
                     /locus_tag="ACGI57_000573"
     CDS             complement(26774..27343)
                     /locus_tag="ACGI57_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9715661.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            27546..27773
                     /locus_tag="ACGI57_000574"
     CDS             27546..27773
                     /locus_tag="ACGI57_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9715662.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(27992..28735)
                     /locus_tag="ACGI57_000575"
     CDS             complement(27992..28735)
                     /locus_tag="ACGI57_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFG9715663.1"
                     /translation="MENYQSTNICTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            28903..29775
                     /locus_tag="ACGI57_000576"
     CDS             28903..29775
                     /locus_tag="ACGI57_000576"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9715664.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29775..30440
                     /locus_tag="ACGI57_000577"
     CDS             29775..30440
                     /locus_tag="ACGI57_000577"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFG9715665.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30461..31318
                     /locus_tag="ACGI57_000578"
     CDS             30461..31318
                     /locus_tag="ACGI57_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9715666.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31474..33225)
                     /locus_tag="ACGI57_000579"
     CDS             complement(31474..33225)
                     /locus_tag="ACGI57_000579"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9715667.1"
                     /translation="MFLYSVGGIVLAIIMLGSIFLIYNSFNMSLNERTHQFGILSSVG
                     ATAKQLRNSVLFEGICIGAIGIPIGVAIGIVSIGLVISVISKNFANILYVNVPLTLTL
                     SIPAIIVAAVVSMVTILISAYIPARKAANTPVMECIRQTNEVKIESKSVKTSKLTQRV
                     YGLEGVLAMKNFKRNKKRYRSIVLSLVLSIVLFISTSALVADLKQMSNQRSEVTDQDI
                     IFSTQGMKDNEMLKLYDKLKTANGVYEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSM
                     SEMLELPVDMQFLDDRTYLNIIKSLGLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSS
                     SMNVSLTPETNGKLEIPERQNVNISFVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKD
                     KFEIPGVSTGIESLSFQSKNPTQSVAEMEQIIESEGVTNEYNLYNMYGMMDENRNYIF
                     IANVFAYTFIIMISLISIANVFNTISTNIKLRRRELAMLRSVGMSEHDFQKMMNFECA
                     FYGMRALFFGLPIAVISSWLIHKVMVTDDIDFVIPWFSIGISIFSVLFIVFITMLYTI
                     NKIKKENIIDALRDDMA"
     gene            33188..33313
                     /locus_tag="ACGI57_000580"
     CDS             33188..33313
                     /locus_tag="ACGI57_000580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715668.1"
                     /translation="MASTMPPTEYKNILNKLLSEIPMNRNTLSLKMEAWSLVLLV"
     gene            complement(33472..34020)
                     /locus_tag="ACGI57_000581"
                     /pseudo
     CDS             complement(33472..34020)
                     /locus_tag="ACGI57_000581"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34020..34706)
                     /locus_tag="ACGI57_000582"
     CDS             complement(34020..34706)
                     /locus_tag="ACGI57_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9715669.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34789..35778)
                     /locus_tag="ACGI57_000583"
     CDS             complement(34789..35778)
                     /locus_tag="ACGI57_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9715670.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(35765..36451)
                     /locus_tag="ACGI57_000584"
     CDS             complement(35765..36451)
                     /locus_tag="ACGI57_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9715671.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(36448..36570)
                     /locus_tag="ACGI57_000585"
     CDS             complement(36448..36570)
                     /locus_tag="ACGI57_000585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715672.1"
                     /translation="MDYIRTKETTENCVMISKIVVYYCSAGIYKTNKIKEDESK"
     gene            complement(36951..>37232)
                     /locus_tag="ACGI57_000586"
     CDS             complement(36951..>37232)
                     /locus_tag="ACGI57_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715673.1"
                     /translation="KSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKMRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT        11906 a         6893 c         4172 g        14261 t
ORIGIN      
        1 tttcgtcaat actttttttg ttttttattt taaaaaaggt tataactttt ggttttttta
       61 ataaatataa ttaaaactaa tagatataac ccttgatatt acaaacaata catgcatata
      121 taattttacc tattttgcaa ttttattttc ataagaatat gaattagctc tcactctatc
      181 tgcccaaaca tttatactct taactttatt aacattagtt gtaccctcaa aaatatccat
      241 tgcatcctta caaaaatata tcataacatc aagaaccaaa aatccaacac aaaataaaca
      301 taaaaaatta attacttgaa acatcagaca ttcccccaat aattttttat atatagtatt
      361 ctcataaatt attcttttta ccttctcata aattattcta aatattttca taataaatta
      421 ctttcaatca tattttctaa atataaagtc tcaatcaaca aaaactaata tcaatttcta
      481 acgtttgttg gtaatttata aattaaatat ctaaaagaat ctttgaagca tataaaaagc
      541 attccacact gacattttaa ataaaattta gctatttatt gataatttaa tagctaaact
      601 ttatttaaaa cttagtagaa tgctaatatt tcttagtttt cttcttcttt atttggattg
      661 ttatcaatag gtttcatagt cgggaataat aatacatctc ttatagatgg agagttagtt
      721 aataacataa ttactctatc tatacctatt cctagtcctc ctgtaggagg tagacctacc
      781 tctaatgctt ttaagaaatc ttcatccatt tcaaaagctt catcatcacc taattctctt
      841 tttctcaatt ggtctataaa tcttcctttt tggtcaatag ggtcattaag ttcagaaaat
      901 gcattagcta actcccactt attagcaaat gcctcaaatc tatcagttct tcttgggtct
      961 tcaacatttc tcttagataa tggagatact tcaactggat ggtgagttat aaatgttggt
     1021 tggattaatt tatcttctcc aaattcttca aagaaagcat ttattacttc tcctcttctc
     1081 attccaggtt ttatctctat acctttttct ctagcaactt ctagtgcctc ttcatctgta
     1141 tttatagtag aaaaatctac accagaatat tctttaacac aatcttccat actcattctt
     1201 ttccaaggtg gtgtaaagtt tatttctgtt ccttgatagt taactatcat acttcctgta
     1261 gcaacttcag ccatatgtgc aataacattt tctgttattt ccatcatatc agtatagtct
     1321 gcatatgctt gatataattc tattgcagta tattcaggat tatgttttaa atccatacct
     1381 tcatttctaa acattcttcc catttcataa actttatcaa atccaccaac aataagtctt
     1441 tttaaataca attcattcgc tattcttaaa tacattggta tatgtaaagt attatgatta
     1501 gttataaatg gtctagcatt tgccccacct gctatagtat ttaatattgg agtctcaact
     1561 tctaaaaatc ctctatcatc taagtatgct cttaatgctt ttaatgcttt tgttctaatt
     1621 aaaaaagcat tttttacttc tggatttact attaagtcaa catatctttg tctatatctt
     1681 agttcttggt cttttaatcc atgatatttt tctggtaaaa cttgtaaaga tttacatagt
     1741 aatactacag atttagcctt tacagatatt tcaccttttt tagttttaaa gatttctcct
     1801 tcaattccaa ctatatctcc aatatcatat gttgaaaata cactatattc ttcctctcct
     1861 atagcatcta gtcttacata agattggatt cttccctctt ggtcttgaat atctataaag
     1921 ccagctttcc cttgtattct cttgctcatt atacgtcctg ctatttttgt agttttaccc
     1981 tcaagagaat caaagttatc ttttatatcc atagaataat gagttctatc atatctactt
     2041 tctttaaaag gatctctacc tgactcttgt aattttttta gtttatctct tcttacttga
     2101 agtacttcac taaggtcttc ttgaatttgc gcttcttcat tgctttgttg attatttttc
     2161 atttttttcc tccaaattat cttcttattt ctaatatttc aaacttagca actccatcag
     2221 gaacttgcac ttccacaact tctttcgcag ctctacctaa aagtgctttc cctacaggag
     2281 attcgtttga tattttacca tcatatgggt ctgcttctgt cgaaccaact atagtatatt
     2341 caacctcttc atcaaattct aaatccttaa ctttaactat tgatcctatt gtaacaacat
     2401 taaggtctat tttgctttca tctatgataa cagcttttct aaccatattt tctaatttta
     2461 aaattctttc ttcaacttgt gcttgttcct ttttagcttc atcatactca gcattttcag
     2521 ataaatcacc aaaagatata gcaactttta ttctctctgc aacttctttt ctctttacaa
     2581 cctttaagtt ttctaattct tcttctaatt tatcatatcc ctcttgtgtc aataaaaact
     2641 ctttattttc ttccattata aatacaactc cttcacaaat ttcttaggaa ttttcctaat
     2701 tatattttaa aaattttata atcttttttt aatttatgcg ctatattata agtcactatt
     2761 atttttatgt caaggagtct tccatatagt actccaattt cgacacaact tcttgatagt
     2821 cttctatttt gttaatttca tcccttaatt ttgcagagtt tctaagtcct tttaaatacc
     2881 aagcgatgtg ttttctcatt tcccttacag ctacatactc tccatgttct tctactgcaa
     2941 gttttaaatg cttaatggca gtacttattt tttcgttaag agttggctct ggcaaaatct
     3001 cgcctgtatt catataatgg tttattcttt taaatatcca agggttacct tgagcacccc
     3061 tacctatcat tatagcatca caaccagttt tgtctaacat atttattgaa tcttctattg
     3121 taaatacatc accatttcct ataacaggta tactcaaatt tttcttaatc tctgcaatta
     3181 tatcccagtc tgctttgcct gtataaaact gttctctagt tcttccatgt attgctaaag
     3241 cacttattcc acaatcctct gctatctttg ctatttcaac agcatttaca ctattatcat
     3301 cccagccttt tcgaatcttt aaagtcactg gcttttttga gttcttcact actgccctca
     3361 atacctcttc tgcaagctta ggatttttca taagtgctga accatctcca tttttaacta
     3421 cttttggagc tggacacccc atatttatat cgagaatctc atttgaataa tcattcatta
     3481 tctctgttgc tcttcccata aactctggtt ctgaaccgaa tatctgaact gctacaggat
     3541 gttcttcctc ttctatctta agcatttttt tagtattttc atcatcatag caaagtgcct
     3601 taccatttat catttctgta tataaaagcc cacatccttg ttctttacaa attaatctaa
     3661 atggaaggtc tgttacacct gccataggtg atagaaaaac tttatttttc aattctaaat
     3721 ttcctatttt cattgttttt ctcctatttt tgcctttatt ttgtgattta actttaaatt
     3781 cttatttcct cattataata taccacatat agctagaaat ctatcataaa aagggaattg
     3841 gatatataac ccaattccct gtatattaat tattcttggt ttttttcata tattattctc
     3901 aatccttcta gtgttaaaaa accatctaca taatctatac ttttcgtctc tgaagcaatc
     3961 agtttagcta atccacctgt agctataacc ttaacatcat cacaattcaa ttctttcttc
     4021 atcatactta ttattttgtc aactaaacca acatatccat aaattattcc agattgcatg
     4081 gctgatacag tactcttaca aatagtcata cctggcttag ctaattctac tctaggtaat
     4141 ttagacgcac tttgaaataa cgcctcacta gatattttta ttcctggtgc tattgttcca
     4201 cccaaatatt cacctttttc agagatagca caaaatgtag ttgctgttcc aaaatcaaca
     4261 agtatacttg gtgctccata cttttctatc cctgctacag cattaactat tctatctgcc
     4321 ccaacttgtt ttggattatc atattttata tttagacctg tttttatgcc tggacctact
     4381 attaatggct gttttttaca gtactttata caaaagtttt caagagaatg cataacattc
     4441 ggaactacag atgatattat aacatcatca atatcactta tattcacatt atcataatca
     4501 aataggttac ttatcaggat tccatattca tcagacgttt tttccctatc tgttttaatt
     4561 ctccagtaat taactaattt gtcaccttta tatataccta aaaccatatt agtatttcca
     4621 acatcaaata ctagaagcat atgtcattaa ccttctttct attttttgct tttcttaagt
     4681 acagtggcaa cagcagctac tactaaagcc cctactattg cttctggcac cccatttgct
     4741 gcggcaagag ccattattac ttttcctgct gaaccacttt tacccattgc atgaagatac
     4801 ttgtcagcat ataagacata tatcattcct aaaactccag ctgtatttaa cagagaacct
     4861 attgcccctg taattaaacc tgatatgtat accttatttg tagccttaat tattaactga
     4921 tatacataat aagctaatac acctatcata actcttggta atattgaaac taatggattt
     4981 ataaaaacaa aagatgttgc tgttgggttt gttatagcat ttaagagact tgatatccca
     5041 aatataaatc ctattgctgc cccaactatt ggtccttcca taatagctcc aataataact
     5101 ggtatgtgca ttatagttgc ttttgtaggt ccaactggaa taaatcccaa tggagtcata
     5161 gatagcataa tagaaatagc tgatagtact cctattatag tcatctttct tacatttact
     5221 tttcttactg atgcacttgt gttcatcata aaaaatacac ctccgttcca gctcattaag
     5281 atgccggact acttaatttt ttaatttttt tcgttttttc ttataactat gagtcaagct
     5341 accattgtag gatgcccgct ctaacaagaa taattttatc aagatgagtt gccattgtca
     5401 acaatttgac aagaaaaata tgctaatttc atcatatttc tcgttttttt actatatctt
     5461 tatatgccat tatattttct acactatccg ctttttttac tgcatttatt atactttttt
     5521 ccacaacctc tacagccaat gaacctacta atgttatatc tgcttctatt tctcctgttg
     5581 atattgtgaa tattgtatct ccatcatgag gtgtatggat aggaaaaata gacttagcat
     5641 atccatcatg tcccatttga gatatctttt tacatccagc tttggttaac ttagcatttg
     5701 ttgcaattat cccaattgta gtattatcta cactaaatcc acctttactt tcaccttgtt
     5761 tcattaattt atatgtatca attgtttctg tctttttatc atttaaagca ccagctataa
     5821 ccttaccatt ttcataaaca tctccaagag catttacagc aattaatgca gatacaatta
     5881 gaccattatc taattttata gaatagctac ctattccgcc tttcatagca tattcctgac
     5941 ctcttatctt tccaacagtg gctccacaac ctgctccata atttccttga tttaaaatat
     6001 cagtatttgc aacttcacaa gctctataac ccatctttaa atctggtcta catttaaagc
     6061 tccctactcc taaatcaaat aaaactgctc ctacaactat aggcacttta cctacaccta
     6121 catcaaagcc aattcctttt tcttctaagt atctacttac accacaagtg gattcaagac
     6181 catatgcaga acctccagcc aaaactactg catgaacttt ttgaaccata tttacagggt
     6241 ctaataaatc ggtttctcta gtacctggag cagagcccct tacatcaaca ccgcaagtag
     6301 ctccttcttc acatattact actgtgcacc cagtaagacc atttctatcc tcaacctgcc
     6361 caacctttaa gccttctata tccaaaatat tattatacat atcctttttc tcctcttata
     6421 gaaacttcac ctgaaataat ttcttttata cttccatcac ttctttgaac taataaattt
     6481 ccctcttcat ttatgtctaa acatttcact agttctctat tatctccttt taatacatat
     6541 atatttttat ctataattgc agaataatct ctacatattt ttaaggtttt ttctttggaa
     6601 ttatttacag tataatctaa atataatttt tcaaattcag ttaatacatt cctaacaata
     6661 tctactctgg ataacatata tccttcttta tacaatgatg tggctttgtc agctatttct
     6721 tgttcaaaat ccattatttt aacatttatt ccaattccaa gaacaacata atttgttctt
     6781 tcaatttcag cagataactc tgttaatata ccacatattt tttttccatt taaaataaca
     6841 tcatttggcc attttattaa tgtatttgct cctagattgt ttaaagcttt cgttacactt
     6901 gctcctgcta ttaaagttat aaatggggct ttgaatggtt ccatatctgg ttttaatata
     6961 atactcatcc agattccttc atgaggtttt gaatcccatc ttcttcccaa tcgtcctttc
     7021 cccatatctt gatgttcact taaaatcaca gtcccatcta cactatttat agctatcctt
     7081 ttagcatgtt catttgttga atctatagtg tcaagatgtt caatattttt tccaataaac
     7141 tctgtatcta atttatagta aatattctgt gggcttaata aatcagttgg attttccgct
     7201 aatcgatacc cttttttatt tacagattct atattatatc cctcttcttt taaggctttc
     7261 atatgtttcc atatagctgc acgtgacacc cctaattgtt ttgataattc ttctcctgaa
     7321 ataaattcag atttattatt taatataatc tctattattt tatctctcat atttcacctc
     7381 taatatgaca taatttcatg acttattttc ttctaataaa taattataca ttattaatat
     7441 acttttaata ctattaattt taaatttcct ttttaattat tataatggtt gttttatatt
     7501 ttatattgat gatttatata attttacatt ttatttaaat aattttaaaa taataaaaaa
     7561 actgaaagta tcaattaaat taatacatgc aaatataatt tgcttattaa tttaatatta
     7621 tactttcagt tttagtattt aatccacatc agataataat cttaaaatga ccaaggtctt
     7681 aaaactattt atctttatca gatttatctt ctaaattttt atttatatca ataacattgt
     7741 ttctttcttc ttcatctttt tctaatttta tagttaattg ttcttcaact tctttagaag
     7801 aattttcctc tgtaactgca ttttcaattt cattcagagg taactcttta ttaaatgctt
     7861 ttacaaattg ttctgcatct aaagtttcat atataagtaa agcttgtgct acatactcta
     7921 atctatccat attttcttgt aatatacttc ttgtcttatc atatgcacca tcaactatac
     7981 gctttgtttc ttggtctatc tcaaaagcaa cttcttcaga ataatttctc ttacttgaaa
     8041 aactattacc taggaatact tcatcatcac tgtcaaatga cattggacca agtttagaac
     8101 tcattccata ctttgttacc atacttcttg cagtagctgt aactctttct aagtcatttg
     8161 aagcacctgt agatacatcc ttaagtacta gctcttctgc tactctacca ccaagtaaaa
     8221 ctataatatt ttctttcatt tcattttttg tagcatagaa cttatcttca acaggaagtt
     8281 gcattgtaaa ccctccagct cttcctcttg gaactattgt aacttgatga acaggactta
     8341 cttcttcaag tacatgggca caaactgcat gccctccttc atggtatgct gtaagtcttc
     8401 tttctttctc acttattact ttcgattttt tagccacacc tgctataacc tttgttatag
     8461 cttcttctat agtttccatc ttaattttct tttctctttt tctagctgtt aatattgcag
     8521 cctcattcat taagttctct atatcagcag gagtaaatcc tggtgttctt ctagccaaga
     8581 catccatttt tacatcatca cttaatggtt tatttcttga gtgaacttta aatatagcct
     8641 ctctaccttt tacatctggt gtaccaacaa caacttgtct gtcaaatctt cctggtctaa
     8701 gtaaagctgg gtcaagtata tctggtctat ttgtagctgc catgattatt ataccttgat
     8761 ttaccccaaa tccatccatt tcaactagaa gttgattaag agtttgctct ctttcatcat
     8821 gacctccacc aagacctgca cctctttttc taccaactgc atcaatttca tctatgaata
     8881 tgatagctgg agcacttttc ttagcctgtt caaataaatc tcttactctt gaagcaccaa
     8941 ccccaacaaa catctcaacg aaatcagaac cacttatact gaaaaaggga actcctgctt
     9001 ctcctgcaac tgctcttgat agatatgttt taccagttcc aggaggtcct accataagca
     9061 ttccttttgg tattcttgca cccaattcaa tatatttctt aggattcttt aagaaatcta
     9121 ctacctcctg caaatcttct ttttcctcat ctagtccagc tacatcttta aatgtaactc
     9181 tagttttttc atcatcttta tgaaccttag cttttgattt gccaaaactc ataacctttc
     9241 cgcctccacc ttgagactga ttcataaaca cgaaccaaat aatcaccatg aagaatatta
     9301 acagtaatgt aggcaacatt tcgacaaacc aaggtgtaga tggcttagct tcaccaccaa
     9361 aagttagttt cccagcctta gcttggtcta acacctcatc tgcaagttta tttcccatca
     9421 cttctgttgg aacatatgac ttaaactttg tatttgtatc ttttatagta ccttctacag
     9481 aagtttgatt tacaaagtat aatcttgata tattctcatc tgttaattct ctatatactt
     9541 cagagaattt taaatctttt attttttctg taggcttacc tgagaattga actataccta
     9601 ctataattat aaaaacaagt agataaaaac ctgccccttt tagcaactta ttcaatatag
     9661 aagccctcct ttcatacatt gctttgccct ataatttgtt aaaattaatt tttaatactt
     9721 cttttgtgct ttcatcaatt ttataatttt cactgatttt ataatctcca acacatatta
     9781 ttccttcacc atctgtgatt ataggaattt tacatctttc ttctcttggg atttttaaat
     9841 ctatgaataa atctttcact tttttacttc ccatagcaag ctttatctta tcaccttgcc
     9901 ttctgcttct tattactata ccccctttta ccttattaaa atcaaaccct ttagatgaat
     9961 tgtctaattt catactctta tacctatcta tgctcattac ttgggtctca ataatttgtt
    10021 ttaattcttt tattttaata aacccattac taggtacatt gtaataaaat tcaatttcct
    10081 cattaacaat ttcctcattc gttaatatta tactgtcttt ttttctataa acaaatatat
    10141 ttcttggaag agttaacatt ttatttactt ttgaattatc ttctaaagac attatatctt
    10201 ctatatgtct ttggtcaaca aaattagtat ctcctagtat atgttttatg gaatttctaa
    10261 gaactctaac tttgatagca tcatgtaagc aaacaaaatc gtctaaattt atctctacaa
    10321 agcctttttc ttttatattt gaaacttctc taaatttagc ttctgcctct ttctcaatat
    10381 aatcgttatc actttttaaa ctattgctca tcctcactat agattctatt acatttgaat
    10441 taaaattatc cttcatatat ggtaaaaggt ctaaccttat cttatttcta gtgtatatat
    10501 tttctaaatt tgttttatct atcctcggat ttaaattata agcttcacaa tactcctcta
    10561 tctcatttct ttccacgtct aatatgggtc taattatgca attatctcta atataatcaa
    10621 ttccctttaa gccttttaaa cctgttcctc tcatgatacg catcataacc gtttcggctt
    10681 ggtcattgag attatgacct attgctattt tattggcttt aatcttatcc ttaatttcat
    10741 aaaacatttc atatcttagc ttccttgctc cttcttctaa tgataatccc tcattttcac
    10801 aatattttgg tacattaata gactttacaa agaatataat tcccatatcc tcacaaagtt
    10861 tagatacata taatgcatct ttttgagctt ctatccctct tatttgatgg tttaaatgtg
    10921 ctgcatatat ttctatatta aaatcttttt ttaatctatt tagtacatgc aataaacaaa
    10981 ctgaatcagg tcctccagaa agacctaaaa ctattttatc gcctttttgt attaaattat
    11041 gtttatttat agtacttagt accttatcaa atatcatatt tatccctctt acacttagtt
    11101 ttattcactt ttaagtatac tttctataaa aaaacgctaa acccttgtat caattttaag
    11161 aaattaaagt ttaatataaa tataataata ccaaataaat taaataaaag gtagtttttt
    11221 cactaccttt ataaaatttc tggaaaatag ccatagttat tatttagtac ctatgtatat
    11281 atctatttta ttcctaccta aaaataaatc ttgtaccttt atattatagt ctattttcaa
    11341 ttttatgtgt tccccttctt gtatatttat atctaattct cttgtatcaa tatcaattaa
    11401 taaagtacct tgtggggtca catactttgt agtactactt tctccacatt taaacagcat
    11461 tgttgagtta atagcaccaa actttttgat actaacttga tttttaagta tctttatagt
    11521 attagttatt tctatatctt cttctttttc cttgtaaata acatatattc catcattttt
    11581 ataaaaaatt ttaccaaagg cagtcatctc tatggtgtcc atatttcctt tttcatcata
    11641 ttgtctagta tttatactta tttttacact caacgatttt tcctccatag attcttcata
    11701 taaactagta tttttgtatt tctacttttt gaatcttatc aatttttttc tttaagaact
    11761 ctcttgctat atctgaaaat ttttcaggta tatctgatac ataatattga taagttccat
    11821 gaatttctgt attgtttata atattttgca cctctaaaat cttttttaaa tcctttgcag
    11881 tctctttagc tggattaaca agttttatat gctctccaac ttcttcacct attgttctct
    11941 ttaaaatagg ataatgagta catcctaaca ctaaagaatc tatatttttt tctttcaatt
    12001 cttgtaaata tatctttgct gtcagtttag ccacttctgt atttgcccat ccttcttcaa
    12061 caataggcac aaataaagga catgctttat taacaatttc tatactctca tctattttag
    12121 atatttccaa gttgtatgct tttgaactta tagttccttc agttcctatt attcctacaa
    12181 ttttgttttt tgtagaactt acagctgttc tagctcctgc ttctataact ccaattatcg
    12241 gtatatcata tttttcttga gcttctttta agcttcttgc agtagcagtg ttacatgcta
    12301 taactatagc ttttacacct tttgttttta aaaagtttat agcttgaaaa gtatatttaa
    12361 ttatagtttc tttggatctt gaaccatatg gtattctggc agtatctcca aagtatatta
    12421 tatcctcatt tggtaatatc ttcattatct cttttagaac tgttaatcca cctaatcccg
    12481 aatcaaaaac tcctattggt ttattgttca tctttataca acacctactt tacatttttg
    12541 tgtttaacaa caacttattt atcgctaaaa tttatattta tattcataat taatatatta
    12601 ttacctaaat gattattata gtttatttta cacaaaaaat aaaggagcta tcttaaatta
    12661 gaaaataatt tctacaaaaa gacaactcct ttcactactt aaaaatttaa atttattttg
    12721 caaacacatg atttcctatt ctcatataag gattcaatct atttatccat ttgcaagtgg
    12781 ctatttctgg attccaaaaa tatactgctt tattagttgg gtcagttcca taaagagctt
    12841 cctgagctgc tttacgtgct gagtctgttg gtgccatctt tattgaacca tcaagcacaa
    12901 ctgagaaagc atttttttga tatatgactt cttctattgt gtctggaaat ctagggtcca
    12961 atactctatt tattactaca gctgctacag caacctgtcc ctcatagctt tctcctctag
    13021 cttctcctgc aaccaactta gataataaca tcaagtcatt atcagataaa tttattactt
    13081 ctttctttgc tatatttttt tcttgtttgt ttactttctt aatattttct gttggttcat
    13141 atgcaaatac ctctttaata ggcataaata taaaagtcaa agctaatagt gtagccatca
    13201 gtttactcca cctgtccatg attaaaacca ctccttacat tcttattttt aaaatttttt
    13261 ctctaaatcc ttgactactt caagtacttt aaacttaatt tttactctac ttgttttttt
    13321 tggtgtatct tgttttatta acttatattc tttatcagtt aaaacttcct tcaacttatc
    13381 atctgtagat ttatctatca ctccattaga ttcatcaaca aaacatagtg gagggaacat
    13441 aacacaccac cagttttttc cttcaccttt cccaataata atctttaatg ctttatattc
    13501 accagctgga agaactatat tagaatattg tttagtaggg aaattgctat attgtatacc
    13561 tacctttaca ctgtaattat atccattttt aagaataata tttttactta tttcttccag
    13621 actactatat tcctttttta taatagcttc actttctttt atactttttg aattttgaag
    13681 ctttggctgt aaatatttta ttacttcatc tctaactttt agtttaagtt cttggtcttc
    13741 atctgtattg ctgtttgcta taacatgaaa tcttattaat ttatctttat aatcttttga
    13801 tatattgtct acttttttta cctcaccatt tattactatt gtcattattg atatgactga
    13861 tataagactt aatattaata gtcccaatct aatcttaatt tttctcattg aaaattcccc
    13921 ctcaagataa atttatatca tcaattatgt ccaattaaat ttaatttatt cataatattt
    13981 tttaatacta ataaaaaaag ctatcttaaa atgattacaa tcattttaaa atagcttttt
    14041 aaaccttata ttttataagt ctttactaat catagcctta tctatcataa actgctctgc
    14101 tttttctata tgctctgtag catacttctt ggatagctca ctatcacctt taattatagc
    14161 atcaagtaaa tatttatgct cttcaacaat attatgtgat gtatcataag cagacatata
    14221 ggtaactcta aatctatata cttgttccca caatgagttt attaattgga ttaatttttt
    14281 attattagtt gctttaaata tacattcatg aaattctaca tcaagtttta ataacgcatc
    14341 tacatcggat tccaaagtgc tttttttaaa ttcttctgat atatctttaa gctttttaat
    14401 atcttcatca ctaattcttt cagcggcaag atatgctgct aagccttcta ggcttgctct
    14461 tacttctaat acatccataa tatcctttaa tgacatattt gccacatacg caccttttct
    14521 aggcaacata acgactaaac cttctaattc aagctttctt attgcctctc tcactggagt
    14581 cctacttact cctaattgtt ctgctagttg cacttccatt agcctttgac caggctttaa
    14641 ttttccttct aagattgctt ctcttaaatt ctcaaagact acatctctta atggcttgta
    14701 attatccaag ttcagtttag ttaaattctc cacaaatttc aacccctttt tgactgctat
    14761 taacaacata cacctgttta tattttttta ataattcttt ttttccaatt agagcatctt
    14821 ctttattttt aaatagtcca aaaactgtag gaccacttcc actcatcata gaacctaaag
    14881 cattattttt catcattact tgttttatat cacttatttc tttgtgttta cctatagtaa
    14941 cattttcaag aatatttacc atgctttctg atacagcttt tatatcttca gattttaaac
    15001 actctatcaa atatttatta tttggtcttt ttttaatatt attcaaatct aatccttgat
    15061 aaacttcttt agtagaaacg aataagtctg gtttgcatat caatatattt gtatcacatg
    15121 gtaaaccttt gatatttgtt agcttttctc caataccttg agctaaggca ggtcttcctg
    15181 atatacaaaa aggaacatct gcgcccaaat taagtcctat ttcttttaat tcatcttcag
    15241 ataatctaag ttcccatagg tgatttaaac ctactaatac tgcggctgca ttagaacttc
    15301 ctccagccat ccctgctgct acaggaatat ttttttctat gaatatttct acaccttttt
    15361 ttatataaaa tttattttta agtattttgg cagctttata aacaatatta tcttcatcta
    15421 aaggaatatc taaactagtg ctttttacct ttatttcatc ttcgtctaat tcttttatct
    15481 ttactatatc gtataaatct atcgtttgca ttatcatttc aacaaaatga taaccgtctt
    15541 gcctttttcc caagacatca atggataaat ttatttttgc tctactcttt agtcttataa
    15601 aatccattct atcctccttg tttaaagaat aatgcatcac taacaataat attatactac
    15661 aaaaaataaa ataatagggt attataatac cctattatac ttaattactt taaatttttt
    15721 aatattaatc tactaatacg actttttttt ctaaaataag acacttccca ggcttaattg
    15781 gttcatcaag ttttatatca ttaagttctg caatttcatt ttctgttgta ttgtattttt
    15841 ttgctatatt ccagaaagta tctccttctt tgcatatata tacgattatg ctaggagctt
    15901 tagataaatc atatacacct tggtcttctc ccttgataat aaaattttct gctttcttat
    15961 ctaatgcttc agtaaatctc ttaattttga taatcaagtc tatctggtcc ctattcaaat
    16021 caacttcgac tttatcaata catgctgtat taaacacaga tgctgtatca gttagattat
    16081 ccatttctat atcatgttca aatggaattt cctcactaat tttataaacc aattttaaac
    16141 cttcaactgg gacaaataaa atttcaactt ttatgattcc ttgtataatg cttttatttc
    16201 cttcaatata actattttct atagacatgg ttggacaaac gcttacaata tcttttattt
    16261 gtatatcatc attatcattt cttatgcctt ctctaaccat aaatgtttct tcactacgtc
    16321 ttaaagtctt atttaattga ataggtttgt ggtcaaattt tattatcttt tgaggcgaat
    16381 atgcatcttg taatacttct cttgtaactt catctgtaac ttttacctta caacaagcca
    16441 tacaatctat ttctaaaaga cctgtgttgc tttcactatt ttgcttgaaa atatgattaa
    16501 aatctgacat agacaacaat acttcttcag tcataccatc actaactcca gggacttcta
    16561 caaattgagt aaactcaata cctactctat ccaactctac aagttcaccc tcatatgtac
    16621 aagctaacgg atttatttct aatactccac caataataac tttattgtca gtcactctac
    16681 tttcttttac tttaacacat ggatttaaac ttattataga ttgaatttcc tctgtgttta
    16741 ttgttatagt atctcttata gaactttctg ctttttctat tccaactata tcttgaaaac
    16801 aaatttcctt tctgtgcttt tgtatgcatt ccacttgcgc cacatctttt acaatgtcta
    16861 acctttgctt ttcaaacaaa cttcccctta tattcataag agctcctact ttaatctttc
    16921 tctcattcat tatagtacaa tccatatgct ctacttctga aaataacatg tattccatat
    16981 cttgaataac attgtctttt tctattactt cgtttatatc tacttttccg tctacattag
    17041 aaatagtatt tttatcatct gcaatataga ttacattata attaaaacta cctctgcaaa
    17101 gtatcttccc atctgccatt tcaatttttt taagtgatat gtatccttct gtttttacaa
    17161 tttcatatac atctaatttt ttgtctggca caactgcctc cgcttcaatg aatgtttgaa
    17221 attttccaaa gtctattcta ttgtcaactt taattacatc tttaattaat tccatagtct
    17281 acctccttaa gtattataaa aatatcacac tatatttata tttaaggagc aaaaaaaaaa
    17341 ttacacctat ttttaaatag gtataatttt aacttatttg taatttatct tgatctctaa
    17401 aaacttgcaa ctttacatta gatgttaata aatctgagta actataagat actctaggat
    17461 atccattact ttcatcatct aactttataa caaacacgct tggataaact ttttctagta
    17521 taccctcttt tgtaataatc tgctttcttc ctttattagc tttcaacaat attttctttc
    17581 ctatatgtct ctctaagctt actcttatct tatctagagt ttgaacagta gccacaatat
    17641 caccttcttt aacgtttata tgtttacata atatcacata taaacatttt tgtcaaatgt
    17701 ttaaactaat tattttatat attttttgca aaaatgtcaa tactattttt gaagatatac
    17761 agtataatta tgttaatttt tgctaatgta atattgtttt tggcattgcc catctaaatt
    17821 ttcctcttgt ttcagaacca ccaattccct taacacctgt aatagtctgc tctataacct
    17881 catcaatagg aactactaca tcaattcttg aacaagcaat tttctccact ataaaaacag
    17941 ggtctaatgc atgtatcatt attcttccag gcgaacttgc atagtttgct cctgcactta
    18001 ttattttttc ataatttgat tggcatgctc ctgcgaatat gacaagatta tccaaatttg
    18061 gttgccattt acgtgcttct tttactgttt taacaaaatt tgaagagttt ctatagttac
    18121 tcatatcttc tatatctcct ctttttattg tcattgcatc atgacctgtt attactagta
    18181 tatccggatt atatttttct agtagtgctc ttacttcttt atactgattt tgctctgaaa
    18241 cagctacacc tactgcaggt attccaagtt gagcatatac atccaagcaa atttttaaat
    18301 attctttatc tccatctatc tgtagtactt ttccaggcat tccatatgtg ttaggattta
    18361 attgaagttt tggaactgct ctagtcattc ttttttgtct ttctttagct tttcttactg
    18421 acttatataa taaattttct acatttttat ctattaatat atcttttata tcaggtgctt
    18481 ttactaattc taggtcatca atataagcat ctgctattac tctaaatgca attcccttta
    18541 gtatagctat tttctcatta ttttcatcta caccaaaaga gactatttta aatactatat
    18601 ctttgttatg tgactttcta gctacaatgt ctcctacctt catagttaaa cctccaataa
    18661 atttttatct tagtttatac tatgttcgat ataaaacttt gtttcctaat gtcaaaaaaa
    18721 catttcttat aatacttctt ttatcactct taaagcattt ttatagtata tcttttctat
    18781 atcatcttca ctaaaccctt ctttttttag aggttcatac agtttaccca tctgagatat
    18841 atcttcaatc tcaacttttg aatctattcc atcaaaatct gaccctagag atacaacatc
    18901 tattcctcca acatttacaa tatgttttat gtgtctaacc atatcttcaa gtttactttc
    18961 acttttatca cttaaaaata agtggaagaa gtttatacct gtaactccac ctttatttgc
    19021 taatacttta atcatatcat cagttaaatt tctagaatga ttcatcattg ctcttgaatt
    19081 agaatgtgta gcaattattg gtttagaaga aatctttgca atttcataaa atccaccatc
    19141 tgaaatatga gaaacatcaa ccaacattcc caattcattc atcttatgta ccacttctct
    19201 tccaaaatca gtaagccctt tttctctata ttctacttta ttatgaggaa agctcaattc
    19261 attcacatga ttccaactta ttgtcatcat tctaacccct aagtcgtaaa atttctttaa
    19321 attttctaat ttaccttcaa gtactgcacc ttcctcaata gataaaagag ctgtaagttt
    19381 accttctgat tggtttttca taatttcttc atagttagta gctaaagcta tttcatcact
    19441 attttttttc atttcctcat gaaatttatt tgccatactc atgcaataat caaaaggatg
    19501 ttttacttct tcagtatcaa caaaaagagc aaatgtttga gctaatgaat cccccttttt
    19561 caatctgtct atatcaacag aatatttatt agacttaagt tcacttgttt ctacattttc
    19621 cattaatttt gctatagtat cacaatgtaa atcaagaaat ttcatattta taccccctgt
    19681 atattttata tttatatcta aaaatagaaa tagttgtcct tatagacaac tattgacaac
    19741 tattttttaa tattcttttt aatattactt aatcttattc ttcttttaag ttaggtatta
    19801 cttttttaac ttcttcaatt tgatgccatg ttttatctat attattttca ctaatatatt
    19861 tttttaaatc agttaaagat gtaaaatctt taactgataa attgtcatca tcatctgcaa
    19921 atcttaaaat aaacatgaga ctcccttact ttccacaaca tttcttatat ttttttccgc
    19981 ttccacatgg gcatgaatca tttcttccta tttttgcttc tttaactatt gttttagact
    20041 tcttataagc ctttattatt tcttttcttt ggtctgctgg tagtatatct tcccaacctt
    20101 gtaaattata tagccaatga gcctcaacag ttaccatatt gttatataat ttttcccaat
    20161 ctatttttat agaaacttct ttatcagctt ctacttcttc taaaactata tcttctttta
    20221 agctttcact tattccatct atgaatccca tgaaaaattc attagtagtt ccaaattttt
    20281 cagccaattc acttacttta ccttcaacaa catccatttt attatttaat atttcattgt
    20341 atattccagc ttcaactttc aaatattctt cccagaattt tacttctgct tcttgacttt
    20401 catgattatc acttaaattt ctccattcag tatataaact catacttttt cctcctaagt
    20461 atacctatat ttttatagtt tttatagtat aaaatctttt agttagattg gaagtttctt
    20521 ctttttgatt ataccatatt ctatttaaag ttaactctta tattatatca taattaaaca
    20581 attcttttaa tacttttata gcatactctt ggtttataaa ataagggctt gtcgcaattg
    20641 ttattacttt ggctttttta attaagtttt ttatcttgta aaatttgagt tcaaatggta
    20701 tatagtccat atcacttgaa aatatatcca aatctatatc aagaacaaac tctgattcta
    20761 cttctaaatc aaatccatat agactatcta ttattattaa ttctgaaaat atgttatatt
    20821 ttaaagcagg ttttataaaa cttcccacat ttaaaacttc atttgtatat ctaaatacat
    20881 cttttagatt atttacatct acatcatagt tctcaggttc tctagtatcc ttatgttggt
    20941 ctacatgcac taacttgcat ccttttgtaa actcaccctt taacaaagat tttgcccaaa
    21001 aataaaatgc atggttatga ttgtcaaaca catatatctt tttatttccc aggacatatt
    21061 cgaccatatt ctccaatcct tttgctttta tttccttatt ttcatctact tcattaaata
    21121 ctacctcatt gcctaacctt acatcattta atgtaccatt tattagtttt ggaacgtata
    21181 tgctcttatt ttctctttta tcatatgaaa atacattatt tcctactggt ttttctatat
    21241 aaaatccact atattcataa ttttccataa aactttattt cctccattgt tatataaata
    21301 tataagttat aattttaaaa attaaaatgc acttattgta aatcatatca tctattatag
    21361 aaaacatctt atataactaa atataatatc ttctctcaat atgtattata caataagtgc
    21421 aaaatatatt tatataaaat atactacatt cctataacaa aaccatcagc ttcaattgga
    21481 aggtaactaa ctcttctatt caactccacc tttccaaaaa atactaccca atatggtcta
    21541 aaaatacttt caattttaac tatttcaatc tctgggattc ctcccataaa cctatgagtt
    21601 aatctcatag ccattttatt tgcctttgct tttactcttc gttcgtcctt atcactatac
    21661 tgcacaatat tacaatccac attttttaca acttcatatg taggaaaatc ttgtactata
    21721 ctaccagaac ctgtagaacc attcactaaa atttttaatt catttatatc attagaatat
    21781 ctcctattct tgaaaatgga tttcgttttg tgaattttaa gtgtgataat tttaaactct
    21841 atataatgaa gtctaacttc atgagcatat ttgttcttta atattagctt actaaataaa
    21901 gaattttttc cacttttagc tatctctata gcttcttctt ttgtaattct tataggtaac
    21961 gctcttatat ccataaaatc attctctttc ttattttttt cttgtcattt ttaaaactct
    22021 atatatataa tcaaaaaata ttacaattac aactgccata actccatatg gaattatttt
    22081 tccaactata atatcatttc tagtcaatat accataaaat ataaatcctg ttattaaaaa
    22141 gtacaataat actagaaaac cgtctattcc aagccatttt atttctaaat ctttttttac
    22201 cttagacact aatttagaca tagcagtttc tccttttata gattattttg tgtatttaaa
    22261 ttttcaccga ttctattacc aatcatccat gcaataaatg aaaaaggtat agctatcaca
    22321 agaggctcta actcaaagct ctgaactgga aatattttaa aataaccaaa agtcactcca
    22381 ccaacaatca ttcctaaaat accacccata gatgttcttc ttccatttcc aagaagccca
    22441 cctactatag gtacaaatgc cgaacccatc caaatacttc ctacaaatat acatgcatca
    22501 tatacatttt caaatctaaa tgctacgcta agccccataa ctgctgctaa acatacacct
    22561 attctagtaa actttactaa ttgtttctga gataactctt tttttgtaat tcttccatat
    22621 atatcatttg ctaatgtagc cccagctgct agataatagc tatccaatgt tgatattata
    22681 gaacctaaaa ttcctattat aaatattact cttattcctt gtggtaatgt atctaaaact
    22741 aaagttatat atccagcagc tggagccatt tctggataag tagctcttac tataagtcct
    22801 gtcatagtta acaccatgtc aaaacatatc catagtgaaa aacaggttaa taaggctctt
    22861 cttccaacct taggagaatc tgctgctgaa aatctttgat aaaaagttgg gtctgaataa
    22921 gctccaaaag aatatacaat taacatcaat gctttcatag ctgacatacc ttttgtaggg
    22981 tgcataagct gtggtgcatc tttgagtgct tctgtaagac cttgaaaccc tcctatttgt
    23041 tcaaattgac ctggcataac catagttaaa ccaaatacca tacaaaaaaa caataccata
    23101 tctgtaatgg caactcccat aagcccacca agtactgtaa gtacaacgac tactcctacc
    23161 attattaatc ctattaattc tttatctatt ttccatgcta cgttacctat agttcccata
    23221 gcagttattt ctccaagttg tgcacaatat ataaacatca aaattgcccc aattatagca
    23281 acatgcttgc cataaaaact ttccagtaaa tcaggaactg ttatattggt tcttatatga
    23341 atccttggac caacccacaa tgctaaaggc attctcccaa tatgtgcagc tattgaccaa
    23401 actgcccatg cagatattcc atatatagcc gcatactcta cactacctac tgtaccaact
    23461 cctccatacc atgatgcaac caaagttcct actattaagg accatggtaa acttcgattt
    23521 cctaggtaat aactttccat atcttttaca tttctagaaa aataataacc tattcctaat
    23581 aatataaatg caaatataac tataatccca ttttcccaaa atgttaacat atctaatctc
    23641 cacattctta tattttaatt tttaacttta atatctaaat atgaacaact ctacccattt
    23701 ccataaaaat aggtagagtt atataataat tataagtatt taagtaatct tgttattaag
    23761 caattttatt gtttaaacct ttattttctc ttttatttat tttatttaaa gcataagtag
    23821 caaatatccc tataactgca catactacag ataaaataaa tatatatgta tatcctgttt
    23881 gtccatattt atctaaccaa ttacctgcaa tagtgtaata aaacgcatct ggagaaaatc
    23941 ctataaatga agcaaatcca actactgctc ctgtagtttc taaagatata ttagactctg
    24001 aaacagatgc aaagtatata cctctaaatc caaataaaat tacacttagt attatcatgt
    24061 ttattacagc tatccatata aatcctgtac tcttaggaat aaccaagtaa gcagctgtac
    24121 ttactgtcat taatataaat cctacaaaca ttactcttat agatgaacca actttatctg
    24181 ttataacacc agcaactgga gatgctccca tttttataac ataagttctt attattgata
    24241 aagcagaaac tagagtcatt ggcattacat aaacttctga taagtatgga cttaaataag
    24301 ttaaacttga gaatactaaa taagttgaaa atattataag accaattaac caagcctttg
    24361 gtattgtaac tacttttaac aatgacttaa tacttaatcc ttcatctgat gctccactat
    24421 cttttttctc ttccactaag aaaaatagaa gtataccaca tattatagat gctgcagaat
    24481 ataaccatac tacatatttg aatcctactg ttatttcagc aaatttacca aagaaataaa
    24541 gacctataaa agaaattaat gtacctgata cacctgataa cccttcataa aaaccaaata
    24601 ttcttccttg ctctgactca cttccttgca ttctaacaac cttaacagag gctgagaaga
    24661 aggttaatat tgtcgtaaat ccccataata caaatattaa aagtatcata ctatatgatg
    24721 ggaatgtaga gaaataaaat ccaagtatcc ctgaagcaat taaagaaaaa gttattaatt
    24781 ttctaactga gaatctatct gcaaaccatc ctcctataaa ataagatacc attcctacta
    24841 atccatataa actcaataag ttccctattt gtgtatgact taatgctaaa gcttgttgca
    24901 ttgggtcata aaatgatgat ttcatatatg gtaaactata catagctgtt gttccaaatg
    24961 ccaatatgaa tattatcaaa aatcttttaa cattattttt gctttccatt gattctctct
    25021 ccttaaaatt ttattgttta taatctcatt catataattt tattttaatt tttctattgc
    25081 ttgtttaaaa tcatttatta tatcatttga agattctaat cctacagcta ttcttattag
    25141 attttcccca ttcatttgaa ctaatgtatg ctcttcccct aagcttgttg ctattgttgc
    25201 aagctctaaa gagttcatta atcttctact agcttggtct cttgtcaatc cactgatacc
    25261 atccttaagt ttaaatgaaa ctacgcctcc acctaaacca ttcatttgag ttttacaaag
    25321 ctcatattga ggatgtgatt ttaatgctgg atactttacc aattccacat tctcttcttc
    25381 ctctaaaaac tctgctactg ctaatccatt ttcacaatgc tttttcatcc tcatatctaa
    25441 cgttttcata ccccttaaaa tcatccatgc actcattggt ggtaaagatg ctcctgtaaa
    25501 acaacataaa cttggccatc taactaaatc tattaattct tttgaacctg ctactgctcc
    25561 ccctaaagta tctccatgtc catttataaa cttagtaaga ctatgtatta ctaaatctgc
    25621 acctaaatct attggtcttt gtacaattgg tgtcccaaaa gtactatcaa ctatagctaa
    25681 gcttttattt tcatgagcaa tttctgctat tgtttttata tctactaagt ctaatgatgg
    25741 atttaaagga ctttctatat aaactatttt agtatttggt ctcattgctt cttttacatt
    25801 ttgtggattt actgcatcta caaatgtaac ttcaactcca aatttagata acaactcaga
    25861 catgaataat ttagtgtgtg aaaatattcc atctgaacta actatatggt caccactttt
    25921 taccaatgct aatatagtag atgttattgc ccccatgcct gatgtactag atatggcact
    25981 ttctgcattt tcaagcatag ccaacttagc ttctaactct gtaagagtag gattacactc
    26041 tcttgtatac acatatcccc atgtcataca caactcttca aaatgctcta ctgtctttgc
    26101 agcaaatgtt gctgtttgac atattggtgt agccaatgca cctgttgttg ggtctgcact
    26161 atgtccccaa tgtattattt ttgtctctaa atcttgatta atattgttac tcattttgat
    26221 ttcctcccat tttgacttat ttatacaacg ttttccctgc caaatttcat caatttagct
    26281 tagtcttaat acattttttc aagtatactt ttttatttgt tataatttct aaaaatatga
    26341 cacttatttt gtttttttac agttctaatt ataaactcta tagtaactat agagtcaata
    26401 tgtttttgat atttttttta caatgtttaa aatagtcaat acaaaaatag gtatatctta
    26461 gaataactaa agtcatccta agatatacct atttttatat cacaatataa ataattacat
    26521 ataagcatta gtttttttat cataaataga tttttcaaga tttatcattt tttcataaat
    26581 atattatagt taaatatttt aatattacct aacatctaca ataaaatata ctcttaaaat
    26641 aaaattatat aattaaaaat actctataaa agctaaaaag tttttctatt cgctacatct
    26701 aaaaataatt agatagaggt ataaatgtat ttagtttatt tatacctcta gtcttttata
    26761 aattatatct ctactaaatt gattttttag cacgaataag tgtgctcttg gagataccag
    26821 tcatcttcac aacctctcta tatggcttag aatttaataa ccccaatgca tattgtatct
    26881 gttgattgct gtatttcttt ggcctaccat ctttaaagtc ctttttcttt ttagcaaatt
    26941 cttttcctaa ttgagtacgt tctattatca tatttctctc aagttctgca actgcaagta
    27001 atgtagtcaa aaagaattta cccattgaag tattttctaa aaggcctata tttaaaatat
    27061 gaacacttac ttctttagca aataaacctt ctattagttg tattccttct gatgtacttc
    27121 tagctagtct atccagctta gttgcaacaa gtaagtctcc cttttttaga gatgatataa
    27181 ttttttcaaa tttaggcctg tttgtagtag tgcctgtata agtttccttt attattttag
    27241 catcattata tttattcaaa atttcttgct gttgctgata caaactgttg ttgtctgttt
    27301 gattttttgt acttacacgg caatatccat atataataat catcacttcc taacataaag
    27361 attttgtaca gaagttaccc agaagcttgt ttttacttat ctttatccat tttactaaaa
    27421 cgatttttgg gtgtcaatac tgttaacttt tgacaccata ataattcctt tataatttgt
    27481 ttgactatgt caaaaattac caatataatg aaattaatga gtttatacaa attggaggaa
    27541 ataatatgaa tagtctgtta aaagaagcaa tagatgaatt aaaaaacatc cattgcgggg
    27601 aagttttttt attaaaagaa ttgttcaaag gctatgaatg gaacaggttg gagatgaata
    27661 tcagattgaa attaggaaaa ctttttttgt atgaagtggt aactaatccc aatttaaatg
    27721 tagaggtcat caataaaaat tctgcttatc agcaaatgta taaaaaaaaa tagtgactta
    27781 aatgtatata atagtataat cttcttattg attcaaaagt taaaactttt aatacaaaac
    27841 aatcattcta ttttaacatt tatcaatatg tctaaattta tataattaac acatacgaat
    27901 agtattgttt aaatcacatt atttaagata gataaatctt aaccctactc cagcataaat
    27961 actaaaatag ggcttagaat tatctatatt tttatcccat aaaatgctta gctcttcctc
    28021 ttgattgaac atatatatct ggggaaatac aatagaagcg taaatttata aaatttccaa
    28081 agtcagaaaa cttcagttca aacttaggta taccaaaact ttcaggtgct atttcgtcaa
    28141 cgccaaaata gatgataatt ccattatctg taagataaaa tgcttggtct tcaggtataa
    28201 ctaactcaac attagaataa tacatttctc tattttcatt tatttgagat ttaatgtaat
    28261 cagtaattat attaatataa tcaactccat ctctaaaaat gtcattaagt aaaagttctt
    28321 ttccagttgt caaatctata ttgtaattat tcaatgtatt gtatttaggc ccatagttat
    28381 tagcaaaccc cataaggttt aatatcatac tcagtacata atttttatta aaagtaactg
    28441 cgtaactggt agatacccaa tagctttgtt ttggaagtga attttgagaa gctgcgttat
    28501 tatattctcg ttcttcttcc tgtaagccat ttttaaaatt atttacagtc attcttacag
    28561 cattgtttat gttatttaaa ataactgggt ttacatacgt ggattgattt ctaatattgt
    28621 atataacagg atactttaca tcatatgaaa aatacttact tctttcaaaa tcttcattaa
    28681 attgtacata taatatatta tatgtacaga tatttgtaga ttgataattt tccattaaaa
    28741 tcctccccct tttataaaaa aatcagatac aatataatat gcttaaaaca gtaataatgt
    28801 taatggtaaa tgtaaagaat agaaagtatc taaaacagca aaaaatgact aattttgtgg
    28861 tataatacat aggattgaga gttattttat aggaggaaaa atatgttatt attagcagat
    28921 aaatggaaag attatgaact tatagatatg ggaaatggag aaaaattaga acgttgggga
    28981 agttatgtac ttagaagacc agacccacag gttgtatggc caatggaatc tgaatgggct
    29041 ttatggaaaa accctcatgg acattatcac agaagcaata aaggtggagg tcaatgggaa
    29101 cacaaaaaaa gatatccaga acaatggact ataaattata aaaacttaaa gtttcacatt
    29161 aaaccaactg gctttaaaca tactggatta ttcccagagc aagcagctaa ctgggattgg
    29221 atgatagaga aaataaagaa agctaacaga cctataaaag tgttgaatct ttttgcatat
    29281 acaggtggag ctactgtagc atgtgcatct gctggtgcag aagtatgtca tgttgatgct
    29341 gcaaaaggta tggttaattg ggcaaaagaa aatatagaat tatcaggtct taaaaatcaa
    29401 acagtaagat ttatagtgga tgatgttgta aagtttgttg aaagagaaat aagaagaggt
    29461 aaaaaatatg atgcaataat aatggaccct ccttcttatg gaagaggtcc taaaggagaa
    29521 gtatggcaaa tagaagaaaa gttatacagt tttgtagatt tatgtatggg agttttatca
    29581 gataaccctt tatttttctt aattaattct tatacaacag gattttctcc aatagttctt
    29641 gaaaatgtat tgtctacaac aataggtaaa aagacaaata caggagatat atacggtggg
    29701 gaattaggaa taccagcttc cagagatggt aagatacttc cttgtggtat atttggaaga
    29761 tgggagaaaa aataatgata aaagtcatat atgaagataa ccatttatta gttgtagaaa
    29821 aaccagttaa catattgtct caaggagata atactaatga taaagatatg gtaaatcttt
    29881 taaaagacta tattaaaaaa aagtataaca aacctggaaa tgtatatatt ggtttaatac
    29941 atagattgga tagaccagta ggtggagtta tggtgtttgc aaaaacatca aaagcagcat
    30001 ctagattgtc tgagcaggta agaagtaaga ctttcaaaaa aacttatcta gctgttgttc
    30061 atggcaagat aaaatctaac tcagatatac taaaagattt tttatataaa aataaaaaaa
    30121 ctaatatggt aactgtagtg aaaaaaagcc ataaagatgc caaagaagct gaacttaact
    30181 ataatgtatt agattttaaa gaaaatctaa gtttagttca agttaattta aagactggtc
    30241 gttctcatca aataagagtt caatttgcaa gcagaaaaca tccattatac ggagaccaaa
    30301 gatatggcaa agaaataaat aagattggac aacaaatatc tctttggtcc aataagatag
    30361 aaatttgcca tcctactaca aaagaaaaaa tggaatttac atgtcatcca cctgacaaat
    30421 atccatggaa tatattttaa atctcaaatg aggtgattat atgagtaaaa aattttcaat
    30481 aggagaaatg tctaaacttc ataaaataag catacagaca ctaaggtact atgaccaaat
    30541 agatttattt aaacctgttc atgttgacca agaaagcaat tacagatatt actcaattga
    30601 ccaattttca caattagata ttataaaata tttaaaatat ttaggtatgc ctttaaaaga
    30661 aattaagtca aagcttgatg aaaataatga agaaattcta aaattactaa caaataaaat
    30721 taatatagtg gatgaaaaaa ttaaagaatt agagcttata aagaaggtac taagcacaaa
    30781 gaaagaaact atggaaaaag gtatgaaagt agatgaagtt ggtaaaatag tgagaaaaca
    30841 tattccacga agaagtatct tatctataga gtatgaagaa ggattaagta gtgaagaaac
    30901 tatggaactt tccagaagac aaatagcaaa tatattagaa gataatatat ctgtgttcta
    30961 tggtggagta agtggacttg tatctataga taaattttta aaagaagata aagttattta
    31021 tgacaatagt tttgttactg ttgaaagaga cttatttgat ataaaatcac aaaaacatat
    31081 aacagaaatt tcatctggtg attttatttg tatgacatat aaagggcctt acaaagaaaa
    31141 ttataccgct tttaagaaac tggttgatta tgttgaatat aaaagaatac ctattgaaaa
    31201 ttatatatat gaaataccta taatagaccc tctgtctaca aacaacgaag aggaacttct
    31261 aacagaaatt caaatacaag ttaaaaagag agaaattgac ttaagatgtc tagtgtagta
    31321 atctagttta cttacacatc ttaatatagt tgtctattat caacatatga tttttctaga
    31381 tagtgattcc taacagtgag attttcaaaa aataggagga aatatggcta atgctaattc
    31441 cctcctattt ttaattaagc catatcattt taattaagcc atgtcatcac gaagtgcatc
    31501 aataatattt tcttttttta tcttattaat agtatacaac attgtaataa atactataaa
    31561 taaaacacta aatatactaa ttccaatact aaaccatgga atcacaaaat caatatcatc
    31621 tgtaaccatt actttatgaa ttagccatga agaaattact gctataggaa gtccaaagaa
    31681 caatgctctc atgccataaa aggcacactc aaaattcatc atcttttgga aatcatgctc
    31741 agacattcct acagagcgaa gcatggcaag ttctctacga cgtaacttaa tattagttga
    31801 aattgtatta aacacattgg caatcgaaat cagagaaatc ataattatga aagtataagc
    31861 aaaaacattg gcaataaaaa tgtaattacg gttctcatcc atcattccat acatattgta
    31921 cagattgtac tcattcgtaa ctccctcact ctcaataatt tgctccattt ctgcaactga
    31981 ctgagtagga tttttcgatt gaaagcttaa actttcaatt cctgtagaaa caccaggaat
    32041 ctcaaactta tctttggctg aataaggagc caatactctg aagcttgctt tcttcacatc
    32101 tgaacttctg attcctggag gagaatcagg tggcactact ttgacaaatg aaatattcac
    32161 attttgtctc tcaggtatct ccaacttgcc attagtttca ggagtgaggc taacattcat
    32221 agaagaactg ttgaaaatat caataatatt attatccttt gttaccatag aaacagcaat
    32281 cagttttgca ttttgtccag tatattcttc tgctggtaat ccaagacttt taataatatt
    32341 cagataagtt ctatcatcaa gaaattgcat atctactggt aattcaagca tttcactcat
    32401 agaatgtgaa ccctcatagt tcaaataatc gtctgtaagc tcacttactt tggcaacaca
    32461 tgaataggtc ataagtttct gatacaaacc ttcgtaaaca ccatttgcag tttttagctt
    32521 atcgtaaagc ttcaacattt cgttgtcttt cataccttgt gtagaaaaaa taatatcctg
    32581 gtcagtaacc tctgacctct gatttgacat ctgttttagg tctgctacta aagcacttgt
    32641 agatataaat agaactatgc ttaaaacaag cgacaataca atacttcgat aacgtttctt
    32701 gtttctttta aagtttttca tagcaagaac tccctcaaga ccataaactc gctgtgttag
    32761 ttttgatgtc ttcacagatt tagattcaat ttttacctca tttgtctgac gaatacactc
    32821 catcactggt gtgttggcag cctttcttgc tggtatgtaa gcagaaatta agattgtaac
    32881 catacttacc actgctgcaa cgatgattgc agggatagac aatgttaatg tcaacggaac
    32941 gtttacatag agaatatttg caaagttttt agatatgaca gaaatcacaa gtccaatact
    33001 gactataccg attgcaacac caattggtat accaatcgca ccaatgcaaa ttccttcaaa
    33061 tagcaccgaa tttcgtaact gttttgctgt agctccaaca gatgaaagta ttccaaattg
    33121 atgtgttcgc tcgttcaatg acatgttaaa tgaattatat attagaaaaa ttgagcctag
    33181 catgattatg gcaagcacaa tgccaccaac cgagtacaaa aacatattga acaaattgtt
    33241 atcagagata cctatgaatc gcaacacatt gtcattgaaa atggaagcat ggtctttagt
    33301 gctacttgtg taagagcgaa tctgatgtgg atttttcaat gtgacaaaaa ggctaagact
    33361 gtcagtcttg acttgtttat cttctttggt tatcaaggta tatcctggtg cagaagattc
    33421 ttcaaatacg ggctttctac agatacccac aactgtataa gttttcttat cctgtggcac
    33481 aaaaacttca ctcccagcag tgtatgggtc actctgacca agcttttcat tttcactcat
    33541 tcgtttccct acatccaaag aaatagtatc ccctactgca aatttgacac caccatctgt
    33601 cgcaacttta cttgaaacaa gaatctctct actattttca ggtaatcttc cagaaattaa
    33661 agttataggc aaagtatcaa aagcttcttt gctaaatcca gcaataaaaa gatatggttt
    33721 atctggattt ctgttacctt caagtgttgc atagccaata ttttcaaatg ttgcagtatt
    33781 tgcaacttca tcattgactc tttgttcctg tacaaaagaa gaatccacat ccaaaaactg
    33841 gacatgccaa tcaccatatt ttataatagc tccatttatc atatagttca tcaaagaaac
    33901 tccaaaggta gtaactgctg taatcatagc agctgacaga acaactccta taattgttac
    33961 aagagttcga gttcgattct ttttcaatcc ttgcagagca actttgttaa aaatgttcat
    34021 taacgattca ccttcctctc atcacgcact acttttccat ctgaaataca gataattcta
    34081 tctgcctgta aagcaatgtt ttcatcatgt gtgacaataa tgagagtttg gtgatattta
    34141 ctatggctct ctttcagtaa ctggataatc tcttgtccat ttttactgtc taagctacct
    34201 gtaggctcat cagcaagcat aactgctggt gcattcatca atgcacgacc aatggcaaca
    34261 cgttgctgtt ggccaccaga aagttgattt ggtaaatgtg tttttcgttc ttttaaacca
    34321 agtaattcta acaagtcatt taaccgttct tgattgacct tacgcttatc cataagtata
    34381 ggcaatgtga tattttccac tacatttaat gtgggtatca gattgtgaaa ctgataaatc
    34441 aatccaactt gtcgcctacg aaaaatagca agtttttcat tgctttggcc atatacatct
    34501 tgcccctcca gatatatctt tccacttgtt ggtacgtcta ctcctgcaat ggaatgtagt
    34561 agtgtagatt taccagaacc agaagaacca atgattgcag taaactcccc tttttcaata
    34621 gtaagtgaga catggtcaag tgcagtaacc ttattttcat tcttaccata aaccttacat
    34681 aaatcttcaa tctttaaaaa cgccattata taagtctcct ttcatatttt ataaatatat
    34741 catagaactt tcaacttaca tttaagtgac atttaaatga gagattcgtc actttggaaa
    34801 acgaataaca aatattgcac caccttgtgg gtgattttgt gcagtaattg ttcctccttg
    34861 tctcataata atcgtcttac aaagtgccaa tcctattcca taaccggctg tgcttgaatt
    34921 tttcccacga taaaatctgt taaatagaca tggtaaatct tcttttttaa agcctgctcc
    34981 gttgtcatga atagtaagtt gagtaaacaa aaagttgtcc tcacaaatga tatcaatttt
    35041 tccattatca cccacactct ccatgcagtt tttaaatata ttttgaattg cttctgaaag
    35101 ccaatttaaa tcacccaaaa tccttactcc ttttggtata tcaatatgta aagtgatatt
    35161 gtgcagttcc atgggaatca aaagttgatg aagtgcagaa ctaatcaaat tattcacatc
    35221 aatttgttct ttttgaaaga ctacaatacc tgcatctaaa cgtgataatt ttaaaaggga
    35281 agtaatcagc cagtccattc gtataagtaa ttcctctgtt tctcgtatca actcttttct
    35341 ctcatcttca tcagaagtat tctctaacag tgacaaaata agagttactg atgtaagagg
    35401 agtgcgaagc tggtgggcta tgtcagctag tgagtcagca aggtgttctt tttctttttt
    35461 cagtgcgtaa ttttgctctc taatgcgtaa cgtcatttta gttatttcac tttgtagaat
    35521 agaaagctca ccctcatctg attcagcaat atacagatgg ttagcattat gaagcacaag
    35581 attaatttgt tctgaaatct gtgcaatact tttgtatcga tttttagtaa acacaaaaaa
    35641 tgctattcca aaggtagtgg cagaaacaat agaaagtatt ccagtcataa tactgattgc
    35701 aaatcccagg gctacagtga gaatagtaat taataagaat aaaatagcaa attttcgaaa
    35761 ctctctattt cgaaacataa cttcccccca atctataccc tgtcccacga acagtcaaaa
    35821 ttatttgtgg acttgaaggg tcatcttcta tcttctctcg caagcgtttg atgtacacag
    35881 tcaatgtgtt gtcattaaca aactcacctg ccgcatccca caattcatca agtaatctta
    35941 ccctcgtaat aatactttta ggattgctaa taaacaccaa taacaaacgg tattctaaag
    36001 ctgaaagaaa aacctcattg ccattctttt tcacaacacc agttgctgta tctacataaa
    36061 gcccacaaat ttcaaaagtt gacccaaaac gcccactttt tcgtagtgct gttctaattc
    36121 gcgcaatcag ttcacgtgga cgaaaaggtt tagtaatata atcatctgca cccatattca
    36181 gtccagtaac aacacttgat tcatcaccgg aagctgtcaa aaagataaca ggaatgtctt
    36241 gcttttcttt gatttctgtg caaatcgtaa acccatttcc atcaggtaaa gaaatatcaa
    36301 taagtgccaa atcaaattta ttcttagcaa tctctacaag tgcttcactt cttgtggata
    36361 catgggttac tataaatcct tctgaattaa gcaaaagaat aagatttttg gcaattgctt
    36421 tatcatcctc aactaaaaat atccgtttca tttactttca tcctccttta ttttattagt
    36481 tttatatatt ccagcagagc aatagtacac tactatttta cttatcatta cacaattttc
    36541 agtagtttct tttgttctta tataatccat gcataatacc tccattgatt tattatattt
    36601 cttataacat aaatacacaa gataagacaa aaaacataat gtaagtgcaa gttttttaac
    36661 tcactaactt ataaacatac actcctacta acactacttt cttgatgaca catcttacaa
    36721 gcttatattc atacatcaat gaaaaattaa atttatccat ttattaaaat aaaaggcaat
    36781 agcttctata gctaatgccc tttatcttaa aatgatttaa atactataca atttttataa
    36841 atggctataa ataaactcgt atagaatata aaatttatct acagtctttt gttatacaaa
    36901 atttataacc tcttatagaa caaatttacc actttgcaaa cttatcactt ttactcagct
    36961 atattataaa cttttaattt atctttgttt aaatgtttct tcttaacttc tactaaagct
    37021 tttagcgtat ccaaagatgt cattatttca gtagaaaatt caatggctgt tcttctcatc
    37081 ttaaatccat ctcttgtaga gtcatttcct ttagttggtg tatttattac tatatcaact
    37141 tgtttatttc ttatagcatc taatatgttt ggtctaggtt cttcaattct atttactata
    37201 tcagcctcta ttccattttc tcttaaactc tt
//
LOCUS       DAYAOX010000014        35883 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.014, whole genome shotgun sequence.
ACCESSION   DAYAOX010000014 DAYAOX010000000
VERSION     DAYAOX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.014"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(359..871)
                     /locus_tag="ACGI57_000587"
     CDS             complement(359..871)
                     /locus_tag="ACGI57_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9715674.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            1321..1797
                     /gene="purE"
                     /locus_tag="ACGI57_000588"
     CDS             1321..1797
                     /gene="purE"
                     /locus_tag="ACGI57_000588"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFG9715675.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDNNLRG"
     gene            1803..2501
                     /gene="purC"
                     /locus_tag="ACGI57_000589"
     CDS             1803..2501
                     /gene="purC"
                     /locus_tag="ACGI57_000589"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFG9715676.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            2513..3880
                     /gene="purF"
                     /locus_tag="ACGI57_000590"
     CDS             2513..3880
                     /gene="purF"
                     /locus_tag="ACGI57_000590"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFG9715677.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            3874..4950
                     /gene="purM"
                     /locus_tag="ACGI57_000591"
     CDS             3874..4950
                     /gene="purM"
                     /locus_tag="ACGI57_000591"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFG9715678.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMERYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAVAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            4944..5537
                     /gene="purN"
                     /locus_tag="ACGI57_000592"
     CDS             4944..5537
                     /gene="purN"
                     /locus_tag="ACGI57_000592"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFG9715679.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            5530..7062
                     /gene="purH"
                     /locus_tag="ACGI57_000593"
     CDS             5530..7062
                     /gene="purH"
                     /locus_tag="ACGI57_000593"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFG9715680.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            7073..8323
                     /gene="purD"
                     /locus_tag="ACGI57_000594"
     CDS             7073..8323
                     /gene="purD"
                     /locus_tag="ACGI57_000594"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFG9715681.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            8348..12154
                     /locus_tag="ACGI57_000595"
     CDS             8348..12154
                     /locus_tag="ACGI57_000595"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFG9715682.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKETSIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            12360..13232
                     /gene="rfbD"
                     /locus_tag="ACGI57_000596"
     CDS             12360..13232
                     /gene="rfbD"
                     /locus_tag="ACGI57_000596"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFG9715683.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            13341..14210
                     /gene="rfbA"
                     /locus_tag="ACGI57_000597"
     CDS             13341..14210
                     /gene="rfbA"
                     /locus_tag="ACGI57_000597"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFG9715684.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            14259..14816
                     /gene="rfbC"
                     /locus_tag="ACGI57_000598"
     CDS             14259..14816
                     /gene="rfbC"
                     /locus_tag="ACGI57_000598"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFG9715685.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            14854..15837
                     /gene="rfbB"
                     /locus_tag="ACGI57_000599"
     CDS             14854..15837
                     /gene="rfbB"
                     /locus_tag="ACGI57_000599"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFG9715686.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            15867..16535
                     /locus_tag="ACGI57_000600"
     CDS             15867..16535
                     /locus_tag="ACGI57_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFG9715687.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVNQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            16544..16972
                     /locus_tag="ACGI57_000601"
     CDS             16544..16972
                     /locus_tag="ACGI57_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HFG9715688.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            16996..17310
                     /locus_tag="ACGI57_000602"
     CDS             16996..17310
                     /locus_tag="ACGI57_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFG9715689.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVKVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            17461..17736
                     /gene="flgM"
                     /locus_tag="ACGI57_000603"
     CDS             17461..17736
                     /gene="flgM"
                     /locus_tag="ACGI57_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFG9715690.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            17741..18157
                     /locus_tag="ACGI57_000604"
     CDS             17741..18157
                     /locus_tag="ACGI57_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HFG9715691.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGNDISMSEVVENSNDGYLKEIYTDLKRILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            18175..19485
                     /gene="flgK"
                     /locus_tag="ACGI57_000605"
     CDS             18175..19485
                     /gene="flgK"
                     /locus_tag="ACGI57_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFG9715692.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            19504..20436
                     /gene="flgL"
                     /locus_tag="ACGI57_000606"
     CDS             19504..20436
                     /gene="flgL"
                     /locus_tag="ACGI57_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFG9715693.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            20452..20844
                     /gene="fliW"
                     /locus_tag="ACGI57_000607"
     CDS             20452..20844
                     /gene="fliW"
                     /locus_tag="ACGI57_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HFG9715694.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            20838..21050
                     /gene="csrA"
                     /locus_tag="ACGI57_000608"
     CDS             20838..21050
                     /gene="csrA"
                     /locus_tag="ACGI57_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFG9715695.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            21072..21467
                     /gene="fliS"
                     /locus_tag="ACGI57_000609"
     CDS             21072..21467
                     /gene="fliS"
                     /locus_tag="ACGI57_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFG9715696.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            21467..21823
                     /gene="fliS"
                     /locus_tag="ACGI57_000610"
     CDS             21467..21823
                     /gene="fliS"
                     /locus_tag="ACGI57_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFG9715697.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            21844..23367
                     /gene="fliD"
                     /locus_tag="ACGI57_000611"
     CDS             21844..23367
                     /gene="fliD"
                     /locus_tag="ACGI57_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFG9715698.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSINSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            23370..23705
                     /locus_tag="ACGI57_000612"
     CDS             23370..23705
                     /locus_tag="ACGI57_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFG9715699.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            23807..24679
                     /locus_tag="ACGI57_000613"
     CDS             23807..24679
                     /locus_tag="ACGI57_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFG9715700.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            24772..26913
                     /locus_tag="ACGI57_000614"
     CDS             24772..26913
                     /locus_tag="ACGI57_000614"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9715701.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            26937..28814
                     /locus_tag="ACGI57_000615"
     CDS             26937..28814
                     /locus_tag="ACGI57_000615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9715702.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            28807..30921
                     /locus_tag="ACGI57_000616"
     CDS             28807..30921
                     /locus_tag="ACGI57_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9715703.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYMESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIKRYNTSEFDRDRIKEKQSEIKHFVIDKHCIEIKNYIDKIYNEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            30930..31925
                     /locus_tag="ACGI57_000617"
     CDS             30930..31925
                     /locus_tag="ACGI57_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HFG9715704.1"
                     /translation="MNNIHINKFEKSCERNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLREQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            32017..33249
                     /locus_tag="ACGI57_000618"
     CDS             32017..33249
                     /locus_tag="ACGI57_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFG9715705.1"
                     /translation="MKRLMILGSIKYFENIVISAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIELYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            33269..34213
                     /locus_tag="ACGI57_000619"
     CDS             33269..34213
                     /locus_tag="ACGI57_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HFG9715706.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ELFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            34286..34714
                     /locus_tag="ACGI57_000620"
     CDS             34286..34714
                     /locus_tag="ACGI57_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HFG9715707.1"
                     /translation="MNRIDKFMDKYNLIRFVSIGNEYIGNLVALESNKVIPFDIKRVY
                     YIYDVPNNVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGV
                     WHEMFDFDEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            34792..>35883
                     /locus_tag="ACGI57_000621"
     CDS             34792..>35883
                     /locus_tag="ACGI57_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFG9715708.1"
                     /translation="MNLLLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHF
                     EEEFANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGAT
                     PVFVEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDC
                     AQSHGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGS
                     EKRYYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKI
                     ENNCTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNI
                     AENYSDKVVSIPLFIGMEDEEVSYIIKIIN"
BASE COUNT        14697 a         3613 c         6544 g        11029 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaata aaatacttta atgaaaaata gctaacttta attgtaggat attcttaata
      181 tatttgaaat aaataaaaat attgaaggat ataatattca actggtatat aaataataaa
      241 tgatagttaa attttacctt agaggctaat taggtgttta gtcaaagcta atgcctttag
      301 aatacaaatg tattctaaag gcatttttac tatgtatttt catttatatt actaaacatt
      361 atctattttt atatacattt ctatgtattt tattttcatc aaatcggtct ataaactcat
      421 ttttttcttc actatatcca atagatataa gtgcataagg aactaatttt aatggtaagt
      481 ctaaagtttg tctaacttta tttaccactt tatttttagg aaatactcct gcccaacaag
      541 cagctagacc ttgattagta acttctaata ataaattttg tgtgcaagca cctaaatctt
      601 gtatccattt accaggtttt aagaatttat ctctatttcc aagcactact atacataatg
      661 gtgcaaattc tatatgcttt gctctatgct gtgactttga gagctgtttc agcagttcct
      721 tatcatctac tataataaat tcccaaggtt gagcattttt tgatgaaggt gcttgcatag
      781 ctgattttag tatttcagtt attttatctt cttctatatt tttatctata tatgttctta
      841 ttgaacgtct attttttata acttctaaca tacagattct cctttaaata aaattatttc
      901 tatttatata atatcattac tttaaatttt agctattact tatataaaga ataaactttg
      961 tgttggtttt atctaagtaa aagtaacatt cagaactaaa aaacaagact ataaatccaa
     1021 agtattaatt aaaaaagaag taataaaaaa taatatacaa gatttaaaaa agagtattgg
     1081 gcttaaaatt taattagaaa accaattttg agataatctg ttttttattt taacaaaaaa
     1141 acataaataa agttcgatac gtagcgaatg aaaaaattac aagaaatatt ttttttaaaa
     1201 aatttcaaaa aagagttgat aatatatgta tattaatata aaatttaagg tgtataattc
     1261 gtataataat acgaatgatt tgtagtgtaa aataacttat aaacggagag gggaagtcta
     1321 atgaaagtag cagtagttat gggttcaaaa tcagattatc caaaattaga agaagggata
     1381 aaactgcttg aaaaatatgg aatagaagtt gtagcaagag cattatcagc tcatagaaca
     1441 ccagaacaac tttcaatatt cctaaaagaa atagaagatg atactgatgt aataatagca
     1501 gcagcaggaa aagcagcaca cttaccaggt gtaatagctt cacaaacatt aattcctgta
     1561 ataggattac ctattaaatc atcaactatg gatggactag actcactttt atcaatagtt
     1621 caaatgccaa aaggaatccc agttgcaaca gttacaatag acttaggttt aaacgcagct
     1681 ttattggcat tacaaataat gactttaaaa tacccaaaat taaaagagga tttgaaatca
     1741 tatagagaag agatggcaca aaaggtgtta gaagacgaca ataatttaag ggggtagcag
     1801 aaatgttatt atatgaagga aaagcaaaac aagtatattc tacagataat gaaaatgaat
     1861 atgttgttta ttataaagat gatgcaacag catttaatgg agaaaaaaaa gcagaaatat
     1921 cttcaaaagg tatactaaat aataagatat caacaataat ctttgaaatg ttaaaagaaa
     1981 ataatataaa tactcacttt ataaaaagtt tatcagatag agaaatgtta gttaaaaaag
     2041 tagagatttt accattagaa gtaatagtaa gaaatattgc tgctggttct atctgtaaaa
     2101 gagttggtct tgaagaaggt gtagttttcg atgagccaat ttttgaaata agctacaaaa
     2161 atgatgctta tggtgaccca atgctaaatg atgattatgc agtggcaatg aagttagcaa
     2221 ctagagaaga gttaaaattc ttaagagaag aaactttaaa aataaatgag ttactaaaag
     2281 catttttctt aaaattaaac ctaaagttag ttgactttaa aatagaattt ggtaaggatt
     2341 ctgaaggaaa tataatattg gcagatgaag tatcaccaga cacttgtaga ctttgggatg
     2401 taaatacaaa tgaaaagtta gataaggaca gatttagaaa agaccttgga gaccttgtag
     2461 aaggttatac ggaagtactt tcaagaatga ataataaata ggaggcaaat gtatgtgtgg
     2521 agttttaggg atttactcta ataaagatgt aactaaagaa ctatattatt ctttatattc
     2581 tatgcaacac agggggcaag aaagctgtgg tttagctctt ttagatgatg gagaaattaa
     2641 gtacaaaaaa gatatgggat tagttggaga tgtttttaag gaaaatgaat tatctaagtt
     2701 aaaaggaaat atagggattg gacatgttag atattcaaca gctggaggaa gtcatgtttc
     2761 taattgtcaa cctttagttg gaagttgtag aaaaagacaa ttagccatag ctcataatgg
     2821 aaatctagtt aatgcaaatt atttaaaaga tatgctagaa gaagatggat atatgttcca
     2881 aactaattca gatacagaag tgattttata tatacttgct agatattaca aaggggatat
     2941 agtagaaagt ttaaaagtga caatggatta tataaaaggt gcatatgctc ttgtaataat
     3001 gagtcaagaa gaattggttg cagttagaga cccacatgga ttcagacctc ttgtacttgg
     3061 taaaaagggt gatgaatata tatttgcatc agaaaattgt gcaatagata tacttggcgg
     3121 agaagttata agagatgtag agccaggaga gataatagta gtcaaagatg gagaactaaa
     3181 atcatatttt tattcagaaa attataaacc agttaagaaa agttgtatat ttgaacacat
     3241 atactttgca agaaatgatg caactataga caatgtaaat gcatatgaat ttagaattaa
     3301 atgtggtgag agattagctc agaatgaaac tgtaaaggca gatatggtag ttccagttcc
     3361 agattcaggt tggccaggag ctattggtta tgcaaatgct agtggattaa aaataagtga
     3421 aggcttagtt aaaaatagat atgtaggaag aacatttata aaacctacac aagaagaaag
     3481 agaaatagca gtaaaaataa aattaaatcc tctttcaaca ataataaaag gaaaatctat
     3541 aattttagta gacgactcaa ttgtaagagg aactacatct aaacaattag taaaatcttt
     3601 aagagaagca ggtgcaaaag aaattcatct tagaataact tctcctccag tagcttactc
     3661 ttgttactat ggaatagata ctccaaatcg ctctaaatta attgcatcaa gtaacaacgt
     3721 agaagaaatg agagaatata ttggatgtga tagtttaaaa ttcctagata tagaaggaat
     3781 gttagatgca acagaacata agtctacttt ctgtaaagct tgttttgatg gagaataccc
     3841 agttaagaaa attgataagg aggaactact ttcatgttaa catataaaga atcaggtgta
     3901 gatatagatg aaggaaatag agcagtagac cttataaaag gtaaaataaa aggaacttat
     3961 gatggaaatg tagttggaga tttaggtaac ttcagtggat tatacagttt aaaagatttt
     4021 gtaggtatga aagaaccagt tttgcttgca tctacagatg gtgttgggac taagttaaaa
     4081 atagctcaga tgatggacaa gcatgatact gttggaatag atttagttgc aatgtgcgtg
     4141 aatgatttaa tttgtcaagg agcaaaacca ttattcttcc ttgactatat agctcttggg
     4201 aaattagtgc ctgagcatat agaaaaaata gttggtggaa ttgcagatgg ttgcaaaatg
     4261 tctggttgtg cactaattgg tggagaaact gctgaaatgc ctggaatgta tggagaagat
     4321 gactatgatt tagcaggatt ttctgtggga atagcagaca aagaaaagat tgtttctgga
     4381 aacaatgtta aatcaggaga tgtattggtt ggtatatctt caagtggagt acacagcaat
     4441 ggattttctt ttataagaaa gatattttta gaaacttaca attataaaat ggaacgatat
     4501 gttgaagaac taggaatgac agttggagaa gcacttttaa caccaactaa aatatatgtt
     4561 aaattagctt tagatgtatt agctaaacat gacataaaag ctgtagctca cataactggt
     4621 ggagggctta tagaaaatat aacaagagtt atacctaaag gattaggact agatatcaat
     4681 aaaaaatctt gggagaagcc tcctatattt aaaatgatag aaggttttaa tgctgttgat
     4741 gagagagaac ttcataagag ttttaacatg ggtattgggc tagttttaat agtagataaa
     4801 gaaaatgctg atgatgttgt aaactttata aataatagag aaaatgacaa tgctgattat
     4861 gtagataaaa aatatagtga attattagaa gataaagctt atataattgg tgaagtagta
     4921 gatagtcatg aaggtgtaga attatgctaa atataggagt tttaatatct ggtgggggaa
     4981 caaatttaca agcagttata gatggtactg agtctggaga aataaaaggt caggttaaag
     5041 tagttatctc aagtaagcaa ggtgcttatg ggctagagag agctaaaaat cataacataa
     5101 aagcaatttg tgaaactgat gaagacaaga taatagaaat tcttaaggaa aataaaatag
     5161 atttagtagt tttggctggt tatttaaaaa taatcagccc aaagctggtt aatgagttta
     5221 gaaacaaaat gataaacata catccatcat taattccttc attttgtgga gctggattct
     5281 atggagaaaa agttcatcaa ggtgttatag attacggagc taaagtaact ggtgctacag
     5341 ttcattttgt tgatgaaggt gctgatactg gaccaatcat aatgcaagat gtagttaagg
     5401 taaatcaaga tgatgatgca aagacacttg caaaaagagt gttagaagtt gaacatagga
     5461 ttttaaaaga aagtataagt cttttttgtg aaaataaatt aaaacttcaa ggtaggagag
     5521 tgtttataaa tgagtaagag agctttaata agtgtaactg acaagactgg tgtagtggaa
     5581 tttgctaaag agttaaataa attagattat gaaattatat ctacaggaaa tacatttaaa
     5641 acattaaaag aaaatggtgt taatgtaatg caagttgaag atgttacaaa ttttccagaa
     5701 atattagatg gaagagtaaa aacattaaat ccatatatac atggtggtat actttataaa
     5761 agagataaag aatctcatgt agaaactgta aatgaacata aaatacattc aatagattta
     5821 gtagctgtaa atctatatga ttttgaagga actttaaaag ctggaaaaag tcatgatgaa
     5881 ataattgaaa atatagatat tggtggacca tctatgatac gttctgctgc caaaaactat
     5941 aaggatgtaa tagtagtagt agatataaaa gattatgatt ctataataga aaaattaaaa
     6001 acagatacaa tgacattaga agacagaaaa aaattatcat ataaagcttt ctcaactaca
     6061 ggaagatatg atgcgttaat atctagttat tttgctggag aagttggaga tacgtatcct
     6121 gacatattaa atttaacttt ccaaaaagaa caaactttaa gatatggaga aaatccacat
     6181 caaaatggtt tcttatattc acagtcaaat gcaaaaaatc caatcttaaa ttatgagcaa
     6241 ttaggcggta aagaactttc ttttaacaac ttaaatgatt tacatggatg tttagaagta
     6301 atgagagaat ttaaagatag tgaagaagta gtttcagtag ctataaaaca tgctaattca
     6361 tgtggggtag gtttaggaaa agatgctttt gaagcatata caaaatgtta tgaagcggat
     6421 aaggtttcaa tatttggagg aatagtagga ataacttcta ctatagataa agctacagca
     6481 gagaaattaa atgaaatatt cttagaaata gtagtagcat atgattttga accagaagct
     6541 ttagagatac ttaaacaaaa gaaaaatcta agaatattaa aattagctaa aatagaaaat
     6601 agtttacaac catatgaaat gaaatattta gatggaaaat tattgataca agatagaaac
     6661 aatatattag cagaaaaaag tgaaaatgta acaaaggaaa aaccaacaga tgcacaactt
     6721 aaggacatgg aatttggtat gagagtagta aaaaatatga aatcaaatgc aattgcaata
     6781 gtaaaaaatg gacaaactct agctttaggt tgtggacaaa catcaagaat ttgggcatta
     6841 aaaaatgctc ttgaaaataa caaagataaa gattttacag gagctgtttt agcgtcagac
     6901 gcatttttcc catttgatga ttgtgtaaca ttagctcatg aatatggaat atcagcagta
     6961 gttcaaccag gtggctctat aaaagataaa gattcaatag aagcttgtga caagtatgat
     7021 atggttatgg tatttacagg aataagacac ttcaaacact aaggaggata ttatgaagat
     7081 attggttgta ggcggtggcg gaagagagca tgctatatgc tggaaactaa gcaaagaaaa
     7141 aaatgtagaa aaaatttatt gtgcaccagg taatgcagga attgcaaacg tagcagaatg
     7201 tgtaaatata ggtgatacta atatagaaga acttttaaaa tttgccaaag aaaatgaaat
     7261 aggtctgaca atagtaggac cagaagtacc actagttatg ggaatagtag atgagtttga
     7321 aaaagaagga ttaagagtat ttggacctaa taaaaaatgt gctcaacttg aaggaagtaa
     7381 agctttttca aaagaattta tgataaaaca taacatacct actgcaaaat ataaagaata
     7441 tacaaactta gaagaagcta tatcagaaat agatagtttt ggatatccag tagtaataaa
     7501 agcggacgga ttagctgctg gaaaaggtgt tgtaatacct gaaaatagag aagatgctat
     7561 agcaacttta aaagaaatga tgtcagataa gaagtttgga gctgctggag ataaaatagt
     7621 aattgaagag tttttaaaag gaatagaaac ttcaattcta gcatttgtag ataatgatac
     7681 aatagttcct atggcaagtg caaaagacca taaaaaagta aacaactatg agcaaggtcc
     7741 aaacactggg ggaatgggaa ctttttcacc tagtgaaata tatacagaag aattagcaaa
     7801 taaagtaaaa gagacagtat tagaaaaaac tttagaaggc ttcaaaaaag atggacttaa
     7861 ttttaaaggt attttatttg ttggtcttat gataactgaa gatggagaaa aagtacttga
     7921 atataatgta agatttggag accctgagac acaatctgtt ttatttagat tagaaacaga
     7981 tttacatgag ataatggaag ctatactaga taataagtta aaagatatag aaataaacta
     8041 tagtgatgat gaagctgtat gtgttatgct tacatcagga gggtatccag acagttatga
     8101 aaaagggaaa attataactg gtcttgaaaa cttggatgat gatatagtag tattccatag
     8161 tggcactaag atgtttgatg gtaatttggt taccaatgga ggaagagtaa ttggtataac
     8221 tgcaaagtca actacagtta aggatgcagc agaaaaagtt tatgaaaaca taaagaagat
     8281 aaactttgaa ggaatgcact atagaacaga cataggaaga taatttttct aaaatggagg
     8341 ataaggtatg ttgaatactg aaaataaaga ctcaatggtt agaagagtat tagttgaaaa
     8401 aagagaaggt tttgaccttg aggctaaagc tttaaaaaaa gacttagtag aaagtttaca
     8461 catagataat atagaaaatt taagaatatt aaatagatat gatgtagaag gaatatcaga
     8521 agaagtttat gaaaatgcag caaagacaat attctctgaa ccaaatttag atgtagtata
     8581 ttatgaagaa ataccaaaat taaatgatga aagagtattt gcaatagaat ttcttcctgg
     8641 tcaatacgac caaagaggtg attgggcagc tcaatgtgta caaattgtaa atcaagggat
     8701 aagacctgct attaatacag caaaagttta tatattatca ggcaaaataa ctgatgaaga
     8761 gttttctaaa ataaaagatt actgtataaa tccagtagat agtagagaag catctttaga
     8821 aaaaccagaa acgttaaaaa tggaaactga aataccaaca acagttgaag tattagatgg
     8881 atttatagat ttagatgaaa atggattaaa aacatttgta agtgaaaaag gattagcaat
     8941 gactttagga gacttacaac acgttcaaaa atactttaaa gatacagaaa agagaaatcc
     9001 aactataaca gaaataaaag tattagatac ttattggtct gatcattgta gacatactac
     9061 ttttatgact gaaatagaaa atgtaaagat agaagatggt aagtttaatg atatagtaaa
     9121 agaagcgtat caaatgtatc ttaactcaag agacaatgta tatgtgaaca gacataaaga
     9181 tatatgttta atggatatag ccactgttgc agtaaaagaa ttaaagaaaa atgggaaatt
     9241 aaatgaccta gatgaaagtg aagaaataaa tgcatgtagt ataaacgtag atgttgaagt
     9301 tgatggtaag atggaaaaat atctagtaat gtttaaaaat gaaactcaca accatccaac
     9361 tgaaatagaa ccatttggtg gagcagctac ttgtttagga ggagctataa gagacccatt
     9421 atcaggaaga agttatgttt atcaagcaat gcgtgttaca ggaagtgctg acccaagaac
     9481 gactttagaa gatactttac ctggaaaact tatgcaaaga aaaataacta cagaagctgc
     9541 tcatggatat agttcatatg gtaaccaaat aggtcttaca actggacaag ttgcagaagt
     9601 atatgatgaa aactttgttg caaagagaat ggaaataggt gcagttatag cagcagcacc
     9661 aaaagagaat gtagtaagag aaagacctga agcaggagat gtaatcgttc ttcttggagg
     9721 aaaaacagga agagatggct gtggaggagc aactggttca tctaaagaac atagtgaaga
     9781 gtcaatctta acttgtagtg cagaagttca aaaaggagat gctcctaacg aaagaaaaat
     9841 acaaagattc tttagaaata aagaagttgc tcaaatgata aaaagatgta atgattttgg
     9901 agcaggtgga gtttgtgttg ctataggaga gattgctgat agcttagata taaacttaga
     9961 cttagttcct aaaaaatatg atggacttga tggaactgag cttgcaattt cagagtcaca
    10021 agagcgtatg gcagtagcca taaaaaaaga aaataaagat aaatttatac aattagcagt
    10081 agaagaaaac ttagaagcta cgcatgtagc tacagtaaca gatacaggat atttaagaat
    10141 gttctggaat ggaaaagcaa ttgtagatat aaatagagag tttttagata caaacggagt
    10201 aaaacaaact actgatgttc atgttactaa agtagatgaa gaaaatacat tctttagctc
    10261 taatgaaata gtaaaagatg ttaagtgtgc aagtatgaaa gacaagttta caaaagtact
    10321 atctgacctt aatgtttgtt ctcaaaaagg attagtagag atgtttgata atacaattgg
    10381 tggtaataca gtacttatgc catttggagg aaagtatcaa gcgactccaa cacaaggtat
    10441 ggttgctaag ataccagtac ttggaggaga aactaatact tctacaataa tgacttatgg
    10501 atacaatcca aaggtaggaa aatggagccc attccatggt gccttgtatg cagtagtaga
    10561 atctgtgtgt aaattggttg ctattggagg aaattatagt acaacaagac ttacattcca
    10621 agagtatttt gaaaaacttg gtaacaatcc agaaaaatgg ggtaaaccat tctcagcact
    10681 tttaggagca ttttatgcac aaagtaaatt tgagatacct gctataggtg gaaaagacag
    10741 tatgtctggt acatttaaag atattgaagt tccaccaaca ttagtttcat ttgctgttga
    10801 cacagtagat gcgaagaaag tagtatctcc agaatttaaa aaagcagact caaaagtagt
    10861 aatgttatgt gtaaacaagg cagaaaatga tgttgtagat tttgaggaat taaagagaaa
    10921 cttagataaa gtaagagaac ttatacatgg aaataaagtt ttatcaacat atgcattagg
    10981 atttgcaggt gtaggtgaag caataagtaa aatggctttt ggtaataaaa taggatttaa
    11041 gtttagtgaa gaggctgaaa aagcatttac agatgataaa ttatttgagg ctagttatgg
    11101 aaatatagta cttgaattag caaatgatga tttaagtatg ttagaaggat acaattatgt
    11161 tgtgcttgga agtacagtta aagaaactag tatatttatc aaaggtgaag aacttgcatt
    11221 agatgaatta tataaagcac attgtagtac tttagagcct atattcccaa ctaaaacaga
    11281 agaagtaaaa tcaaagatag aaactataag ttatatatca caaggagaag ctaaaaaatc
    11341 atcattaagt atagctactc caagagtatt tataccagca ttccctggaa caaactgtga
    11401 atatgactct gcaagagcat ttgaaagagc aggagctaat gcaagtataa gagtatttaa
    11461 gaatttaact tataaagaca tagaagattc tatagataca atagtaaacg aaataaaatc
    11521 ttctcaaata ataatgttac caggtggatt tagtgctggt gatgagccag atggttctgg
    11581 taaatttata gctactgtat ttagaaatcc tagagtgcaa gaagctataa atgaattttt
    11641 aactcaaaaa gatggactaa tgttaggtat ctgtaatgga ttccaagtat taattaaatt
    11701 agggttagtt ccatatggag aaataagagt tccaagtgaa agtgctccaa cacttactta
    11761 caataatatt ggtcgccatc aagctaagat tgctagaact agaatttcat ctaataaatc
    11821 tccttggtta gctcaaacta atgttggaga tattcataat atagcaattt ctcatggaga
    11881 aggtaagttt gttgccagtg aagatgttat gagagaatta attgctaatg gacaagtagc
    11941 aactcaatat gttgacttta ataatgaagc tacttatgat attgagttta atccaaatgg
    12001 ttcattctat gctgttgaag gtataactag tgctgatggt agagtgtttg gtaaaatggg
    12061 acattctgaa agaattggtg aggaagtata taagaatatt ataggtgaaa aagaccaaaa
    12121 gatatttgaa tctggagtta aatattttag ataaagcttt gatgtttaat tgatagctag
    12181 aatatataat ttagatattt agatttaaag agatgtaata atatgatgat aatcatttaa
    12241 tattacatct cttttttatt tatctaagaa ataataagtt caacctaagt taaaatacaa
    12301 gctgtgttat aattaattat taagtaaaat tgaatattta ttggaggaca acataattaa
    12361 tgaaaatatt aataacaggg tctaatggtc aattaggaaa agaattggtt aaccaattag
    12421 aagcaatcaa tcaaagtata aaccaaccaa aatatgttat attagctaca acaagaagtg
    12481 atttggacat atccaatcag actaatgtag ataattttat tttgcataat aaacctgatg
    12541 tagttgtaaa ctgtgcagca tatacaaagg ttgatgcatg tgaagataat atcgaaattg
    12601 catacaaaat aaatgcttta ggagtgagaa atctagcaat agcatcagaa aaagttaatg
    12661 ctaagcttat tcacatatca actgactatg tattcaatgg tttttctaaa tatccatata
    12721 gggaagacaa taaaactgaa cccaattctg tatatggaaa aagtaaactt atgggagaaa
    12781 aatttgttga acaattctca cataagtact ttatattgag aacagcatgg ttatatggta
    12841 atgggaataa ttttgtaaag accatgataa aactttcttt agaaaataaa gaggttaatg
    12901 tggttaatga ccagtttgga actccaacaa gtacagtaga cttagcaaaa attataataa
    12961 aaatcatgga aacagaacat tatggagttt atcatgctac atgtgaaggt gagtgtagtt
    13021 ggtatgattt tgccaaaaag atatttaaac taaaaaatat agatataaaa gtaaatccaa
    13081 ttaagagtaa cgaatttaaa tgtaaggctc caagaccact atattctgta ttggataatt
    13141 ttatgttgaa gttaattggt ttaaattctt ttagaaagtg ggaagaatcc attgaagaat
    13201 atttagaaag atatgatata aatgaaaagt aaatgtatta ttttgagaaa agaaaataag
    13261 atattataaa aaatataaat atttctttta tttatccgat aatcataata tgacaaaaac
    13321 aaatttaagg atggaaaaag atgaaaggaa ttattttagc aggagggtct ggtacaagac
    13381 tatatcctat aacaaaatgt atatcaaaac aaacacttcc aatatacgat aaaccaatga
    13441 tatattaccc tatgtcggtt cttatgttgg cagggataca agatatatta ataatatcta
    13501 caccaagaga tataaaagca tttgaagaat tatttggaga tggaagttca ctagggctta
    13561 acataacata tgcaattcag gagaaagcaa atggaattgc agaagcgttt ataataggag
    13621 aagaatttat aggaaatgac agagtgtctc ttgtattagg ggataatata ttttatggat
    13681 atggatttag tgaaaggttg gagaatgcag ttaaaagaaa aggagcaact atatttgggt
    13741 atcatgtaag taatccaaat tcgtttggag ttgtagaatt tgataaggat tttaatgtat
    13801 tgtcaataga agaaaaacct acagttccta aatctaatta tgctatacca ggattatatt
    13861 tttatgataa tgatgttgta gagatagcaa agctagttga accgtcagat agaggtgaat
    13921 tagaaataac ctctataaac agtgaatatc taaaacgagg aaaactaaaa gtggaacttc
    13981 taggaagagg tatggcttgg cttgatactg gtacacataa aggtctattg gatgcttcta
    14041 attatgttga agtggttcaa acaagacagg gactgtatat agcatgttta gaagagattg
    14101 catatacaaa aggatatata agtagagaac aattaatgaa actagccaag ccactgataa
    14161 aaactgaata tggtcaatat ctattaaaat tggctagtga agatatgtaa tatggtagat
    14221 aaaatttatt ataaatttta ttaagggagt ttattataat gggaaaaatc aatgttataa
    14281 agactaaaat agaaggatta tatatagtcg aaccaaatgt attttatgat gaaagaggat
    14341 attttatgga aagctataat aaaaaggatt ttatagagca gggtcttgat atgtgtttta
    14401 ttcaagataa tgagtctagc tcaagaaaag gcgttttaag aggaatgcat tttcaggtta
    14461 aatatcctca aggaaaatta gtcagagtta cagagggaga agttttcgat gttgcagttg
    14521 atttaaggaa agattcacaa acatttggga aatgggaagg tgtaatattg tctgataaga
    14581 ataaaaaaca attttatatt ccagaagggt ttgcacatgg atttttagtt ttatcagaaa
    14641 aagcagtatt tagttacaaa tgtacaaatt attattaccc agaatttgag aatggcttta
    14701 tatggaatga caagcaaata tctattgagt ggcctttaaa aggaattgaa gaaatatttt
    14761 tatcagaaaa ggacaaaaaa caaaaaagtt ttaaagatat ttgtacaaga cgttaataaa
    14821 aatttattag aattaacatt taggagaaaa aatatgaaaa aaatactgat aactggagga
    14881 gcaggattta ttgggagtaa ttttatacat tatatgctta acaagtatgt agattatttt
    14941 attgttaacc tagacttgtt gacttatgct ggtaatttag agacattaat agaaatacaa
    15001 gacaattcca attataaatt tgttaaagga gatatttcaa atagggagca ggtgtataag
    15061 ctatttgaag aagaaaggtt tgatatagtt gttaactttg ctgctgaatc tcatgtagat
    15121 aggtcagttg agaatcctga tttatttatt aaaacaaaca ttataggaac acaagtactt
    15181 ttagatgcat ctataaaata tggagttaaa aggtatcatc aaatatctac agacgaagtg
    15241 tatggtgatt taccaattga tagaaaggat ttattcttta cagaacaatc ttctatcaat
    15301 ccaagttctc catattcagc ttctaaagca tcagcagatt tattggtaag ttcttattat
    15361 agaacatatg gattgttaac aactatatct aggtgttcca acaattatgg gccatatcat
    15421 tttcctgaaa aattgattcc tcttatgatt ttaaatgctt tggaaaacaa acaattgcca
    15481 gtatatggga atggggaaaa tgtaagagat tggttacatg tatatgacca ttgtactgct
    15541 atagacttaa taatacataa aggaaatata ggggagattt acaatatagg aggtcataat
    15601 gaaagaagta atctagaagt agtaaaaatg atattaaatc ttttaggtaa atctgaagag
    15661 ttaatatcct atgtaaatga tagacctggt catgatttaa gatatgctat tgatgcaact
    15721 aaaatagaga atgagttagg ttggaaggca aaatatgact ttgatttggg tataaaagaa
    15781 actgttaagt ggtatataga aaatgaatct tggtggaaag ctgttttatc agtataactt
    15841 taaaatttag aaaggggcgt tttatattga atgagataag ttcactaatt aatatattag
    15901 cattgactca attatctggt agtaatacta caaatcaatg taattgtaca tataataatt
    15961 catctggatt tgatatgatt atgatgacct tgttaaaagc attatcacaa aataatcaac
    16021 atacttctaa tagctacaat ttattgtcta attctgagaa tttatttaat gaacttgata
    16081 atgtagtaaa taataccact agtagattta tagatgttga tacaaaagat aaaaatgtaa
    16141 agttgagaat tgaaaatgct gtaaatcaag catcaaaaaa atataacgta gattcaaatc
    16201 ttataaaagc aataattaaa gttgaatcag attttaatcc aaataccgtt tcttctgctg
    16261 gtgctaaggg tcttatgcaa cttatgccag aaaactgcag agacttaggg gttacagacc
    16321 cttttaatat agaacagaat atagatgctg gaactagaca tattaaggaa tacattgata
    16381 tgtttggagg gagcatagaa atgggattga tggcatataa tggtggtcct ggaagaatga
    16441 aaagtagagg agtagaatca atctctgacc tgtataaaat gccaaaagaa acacaaaatt
    16501 atattcctaa ggttatgaaa tattatagag ggtaggcttt attatgggaa taaatataga
    16561 taagctaaac ataagtccaa gtataaatat ggaggttaaa aaactaaaag agagtaataa
    16621 attttctgac aaattaaact ctttaaaaca atcttcttca ttggaagata taaaacgaaa
    16681 tttggaaagt gtaaaaaagg caggagaaag gcttgtactt actcaaaact atggcgatat
    16741 tagtatatat aaaaatgcag taaaagaata cctaaaatct gttgtagata atatgtattc
    16801 acttgataaa aatagtagct tttgggagca tcagtattat aagaatgtaa agattgttga
    16861 tagtaaactt gaggatatga ctagtaagtt aattagtgaa gaaaaagaaa acatagatat
    16921 tgtttctact gttgacatga tacaaggctt attgatagat atgtatagat aaggttatac
    16981 aaggagaaaa attttatgaa tagcaaaaaa gaggatatat atgaattgaa ttttgggcaa
    17041 ttaatagaag aaaaatctgg tgttatagaa gaaaatcaaa atgtttttaa tgcaaaagta
    17101 aaggtagctg tttctatagg aaatacaaca gaaacaataa aaaatatatt agatttaaaa
    17161 gaaggctcta taataaaatt aagtaagaat gtggatgaaa aattagatat atatgcaaac
    17221 gatagattgt ttgcttatgg agaaagcatt attacaaatg agaagttatc tgtaagatta
    17281 tctaaaatag aagattcaga tgtaaattag cattttaaat atatcccttt agtctattct
    17341 gtatgagttt agctaagggg atatttttat aaaaaattta aaatatgtgt gaattatata
    17401 aatattttta ttttatgagc gataactaaa ttataagtat gtaattttgg aggtgaaact
    17461 atggatatta aaaatgtaag ttcaaatatt gtagtgaata attatttaaa gaatacatct
    17521 ggtataaaaa aagtggaaga ggttaaaaaa attaataaaa cagacaagat agagttatct
    17581 aaatttgcaa aggaaatggc aagtgtgtca aatatgagtg atgagaatat tagaaataaa
    17641 aaagtcgatg aaataaaaaa tgcattagaa aatggaactt acaaaataaa accaaaagaa
    17701 attgcgaaaa aaatgataga ggatatgcga ggataaagtc atgattacag acttaaaagt
    17761 tataatatat gaagaaaaaa atataattag taagatgatg gaattattaa aagaacaatt
    17821 tgattatata gttgaaaaag acttagaaaa tttaaatatg ttaaatccta aactagaaga
    17881 tattagcagg gagcttgcat ccatagaaat aaagagaaga caactttttg gtaatgatat
    17941 ttcaatgagt gaagttgttg aaaatagtaa tgatggatat ttaaaagaaa tatatacaga
    18001 cttgaaacgc attcttgctt tagctaaaaa tcaacaagaa tcaaatgatt ctctaataaa
    18061 gaaagaactt atttttacga aaaaaatgat taatttcata aaaccagctg ataagcaaac
    18121 tacaacttat aattcttatg gaaatattag aaaataaata ttaggaggag aaatatggca
    18181 ggattatttt ccattttaaa tacagctaat tcaggaatga atgcgcacca aaaagcaata
    18241 cagacaattt ctcataatat aagtaatcta gatacagacg gttattctag gcaaaggacc
    18301 gagtttgcta caaatagtcc aatgtatatg cctagtttga gtaattcaat tgcaagagga
    18361 caattaggga ctggagtaca tgtaacggat gtaacaagag ctagaaatag tttttatgat
    18421 taccaattta gggcagaagc tcataagtat ggtaagacag tttcaaaata tgattattac
    18481 aatactatag aaactatact aaacgaacca tcagactatg gaatatctgc aggtatagat
    18541 gattttttta aaggatggga gtcattatca aaggatataa atagtgacag taaaaaaagt
    18601 ctagtggtag aaaacgcatc taatttagca actttaataa gtgaaagtta taagaagctt
    18661 gataagttaa aagaagatat agattctaat atagacactg agataaaaca aataaatgac
    18721 atgttaaaaa gtcttgaaga tttaaataaa tctattgata ttatatctgg ttctggctct
    18781 acaccaaatg atttgcttga tgaaagagat agaattttag ataacttgag ttttaaacta
    18841 gactttgaaa atagtgatgt taaaaatatg cttgcagatg gtaaattaga acttaatgaa
    18901 ttaaaaaatc cagatggaac ttggaaaaca ggtatatcag ggaccttaca aggcttgttt
    18961 gaaatgcatg gtaagattga tacatataaa agcgatttaa aagatgtatc tgatggatta
    19021 gctaaacaga taaatgatgt ttataatacg tctgcaggaa taacagtaac agattttttt
    19081 ataacttcaa atgtagatgg tgaagatatt ataaagataa atccagctat aaagagtaac
    19141 ccaaatgaat taaagcttac tgctgatgaa gcttcaaaaa ttgctaagtt aaaggatgaa
    19201 aaaatagata ttggggtggc tggtggaaaa gtaagtacaa taagtgacca ctataaagcc
    19261 tttgcagaat caatcggttt agattctcaa aaagtaaatc aagatgaagc aaatcaacgt
    19321 aaaattataa ataatattga taactcaaga atgagtgttt caggtgtatc tttggatgaa
    19381 gaaatgacag agcttataaa aatacaacgt tcatatcaag ctagtgcaaa agttatgtca
    19441 acagctgtgc aactactgga tgttgtaata aatggaataa tttaatagaa gggggaattt
    19501 ataatgagag tcagtacagg tatgatgtct agcagctatc taaactcact acaagataat
    19561 ctacaaagat tggataaggt taatagacaa gttaatacaa caaaagaaat aaacaagcta
    19621 tcagataatc catataaagc aataaagata ttaaactcaa aaagtgaaat aaagactatg
    19681 gagacttatg ttgagaattg taaagataca gggaattggt tagaaactac tgatactgca
    19741 ttagatcagc ttggaaaatt attatcagac ataaagaaaa ctttggtggc aagtggtgat
    19801 ggtgcatact cagatgatga attaaagact cttagtaatg aaactaatga aaaaatgaaa
    19861 gaaattgcaa atgctttaaa tgcaacacat gaagggaagt acatattttc aggttcaaat
    19921 acgggggtac cacctgttga atgtatagat aatgctgatg gttcagtgag tttaaagttt
    19981 aattctagtc taaatcttaa taaattaaat gactctttga gtatggatat agctcaagga
    20041 ataagtgtag attataacgt taaattgagt agccttggat ttgacccaac aaaaactgac
    20101 ccatttgaag agttaaataa catatcaaaa aaattagcaa atccagatgc tgatgctata
    20161 aaagagctta caacaacttg tttgggcgat atggaacaac taatagaaaa tacagtaaat
    20221 gttaggtcta tatatgggac aaaagcaaat acagtagagg ctatgaagga aaaaaacgat
    20281 gatagtttga tacagttaaa ggatattttg tcacaaaatg aagaaataga ttatggtgaa
    20341 aaacttgttc aattaaaggc agcagagtta acttatcaag cttcactaca aacaggagga
    20401 aagttattta atgtatctat attagattac atataataaa ataacgcaga tatgatgaag
    20461 gttacattaa aaaaaggtat attaggtttt gaaaatttaa aagaatatga gcttttagat
    20521 atagaagata atgattattt aaaagaattt aattctactg aagaagaagg tataggattt
    20581 gttgttgtat ctccatttga gattatcaat gaatatgaaa tagttttaaa tcaggaaaca
    20641 atagaaaaac tagaagtgaa atctccaaag gatgttgtat tatttaatat aattactctt
    20701 ggtcagacat ttgaagaatc aactgtgaac atgaaagctc ctatagtgat aaatataaaa
    20761 actaattttg gaatgcaaat aattttacag gatgataaat attcaataag gcatccatta
    20821 ttgagaggtg ataatggatg ctagtaattt caagaaaaaa agatgaagca gtacttatag
    20881 gagaaagcat agaagtaaaa attgtaggag tagatggaaa caatgtaaaa ctagctatat
    20941 cagcgcctaa caatataagt atattaagaa aagaaatata cgaaaaagta aagagtgaga
    21001 atataaaagc tacaaataaa aatataaaaa ttttaaagtc attaaaataa taagcttgga
    21061 ggaagtaata tatgtatgga gaaaatcctt ataattctta taaacaaaat gctgttttta
    21121 tggcatcaaa ggaacaattg ttacttatgc ttgttgatgg agcagtaaaa tataccaaaa
    21181 tagccagagc agctatctta gataaaaata cacagaaagc gcatagagaa cttgtaagag
    21241 ttcaggatat atttacagaa ctaatggtta cattagacca aaatgcagga caatgggcaa
    21301 aggatatgta caaagtttat gattttgtaa gatatgaact ttcaagagcc aatattagaa
    21361 aagacatcca ggtaatagat aatgtgttac ctgtaataga acaaattaaa gatacatggc
    21421 atgaagctga taaaaaaagt aaagaagaaa gaagtaggta caaataatgg aattaaattt
    21481 agctgaatta gataagttat caaaagagga attactattg atgcttgtag atggcacagt
    21541 aaaatatacc aatatatcca aagaagctct tttaaataac gactatttga aagcacataa
    21601 tgaacttgta agagttcaaa atatatttac agaactaatg actacattag accaaaatgc
    21661 aggacaatgg gcaaaggata tgtataaagt ttatgaattt ataaaatctg aattagcaat
    21721 agctgatgaa aataaagata ttaaaataat tgatgatata ttgccaatag taaaacaaat
    21781 tagagataca tggcatgaag tttacaatca actaaaaata taattagatg gagggataaa
    21841 ggtatgtcaa gtataagtcc tataagagtt acaggtcttt caggaaattt tgatatggaa
    21901 ggcataatcg aagctagtat gattagagac aaggaaaaag ttgataaagc aaaacaagaa
    21961 caacaaatcg ttaaatggaa gcaagaaata tatagaaatg ttatacaaga atcaaaagat
    22021 ctttatgata aatatctaag cataaattct cctaatagta tagtaagtga aaaagcatac
    22081 tcttctacaa gaataaccag ttctgatgaa agtattatag tagcaaaagg ttcagctggt
    22141 gcagaaaaaa taaattatca atttgcagtt tctcaaatgg ctgaaccagc aaaatttact
    22201 attaaattaa attcaagtga acctattgtt cgacagttcc ctccaaatgc cagtggagcg
    22261 agttctttaa ctataggaga tgtaaatata ccaatatctg aacaagatac tacaagtact
    22321 attgtaagta agataaactc cctttgcgca gataatgata taaaggcttc ttatagtgag
    22381 atgacaggtg aattgattat ttcaagaaaa cagactggtt cgtcatcaga cattaattta
    22441 agagtaattg gaaatgacaa tttagctcag caaattgcta atgataatgg tatcacattt
    22501 ataaatgatg ctggtggaaa caaagtggca aatgtatatg gaaagaatct agaagctgat
    22561 gtaactgatg aacatggaag agtaactcat ataagtaaag aacaaaattc atttaatata
    22621 gataatattg actataatgt aaattcaaaa ggaactgcaa agttgacttc tgtcactgat
    22681 actgaagaag ctgttaaaaa tatgcaagca tttgtggatg attataataa actgatggac
    22741 aaggtctatg gtttagttac tactaaaaaa ccaaaagatt atccgcctct tacagatgcc
    22801 caaaaagaag atatgacaac tgaagaaata gaaaaatggg aaaagaaagc taaagaaggt
    22861 atacttagaa atgatgatga gttaagaggt tttgttgaag atattcaatc tgcatttttt
    22921 ggagatggaa aaaatattat tgcattaaga aaactaggta tcaatgaaag tgaaaattac
    22981 aataaaaaag gtcaaatatc atttaatgca gatacttttt caaaggctct tatagatgat
    23041 agtgataagg tatacaaaac actagcaggt tattcttcga attatgatga taagggaatg
    23101 tttgaaaagc taaaagatat tgtatatgaa tattctggaa gttcaacttc taaacttcct
    23161 aaaaaagcag gtatagaaaa aactgcttct gctagtgaaa atgtatattc aaaacaaatt
    23221 gcagagcaag aaagaaatat aagcaggtta gttgaaaaaa tgaatgataa agagaaaaga
    23281 ctttatgcta aatattcagc cttagaatct ttgttgaatc agtattcttc ccaaatgaat
    23341 tatttctcac aagcacaggg taattaatta tggataaatt aaaagaaaaa ttaaatttat
    23401 ataaagatat atctttacaa attataaatt tgattgaaaa ggaagaatat ataaagataa
    23461 gtattaagtt aggtgaaaga caagagatta taaatagtgt aagtgaaatt gatagaaatg
    23521 attttataca gctttataat agaatggaat taattgaaat tgatagcagg ataagagata
    23581 ttttacaagg acaattatta gaagtaaaaa aagaattaca tgagtataag cttactaaac
    23641 aagtgaacac aatgtattat aacttaaata gagaaaaagt aaatattttt aataaaaaag
    23701 tataaagtta tgtaccatct tgtccgatta tataaatgaa gaattaataa aaaagattaa
    23761 aagtagaaat gacaaggatg tcaactatac taaggagggt aaaataatga gagttaatac
    23821 aaatgtaagt gctttgatag caaataacca aatgggaaga aacgtaaatg cacaaagtaa
    23881 gtctatggag aagctatctt ctggtgttag aattaagaga gctgctgacg atgctgctgg
    23941 gcttgctata tctgagaaaa tgagagctca aataaaaggt ttagaccaag caggaagaaa
    24001 cgttcaagat ggtatatctg ttgtacaaac agcagaaggt gcattagaag aaactggtaa
    24061 catactacaa agaatgagaa ctttatcagt acaatcttca aatgaaacta atactgctga
    24121 agaaagacaa aagatagctg atgagttact acaattaaaa gatgaagttg aaagaatatc
    24181 aagctcaatt gagtttaatg gtaagaaatt acttgatgga agttctacag aaataagatt
    24241 acaagttgga gcaaactttg gaacaaatgt tgcaggaaca tctaacaaca acaatgaaat
    24301 aaaagttgct ctagtaaata cttcaagtat aatgagtaaa gcaggtataa caagttctac
    24361 aatagcaagc ttaaatgcag atggaacttc tggaacaaat gcagctaaac aaatggtatc
    24421 tagcttagat gtagctctta aagaattaaa tacatcaaga gcaaaattag gggcacaaca
    24481 aaatagacta gaatcaactc aaaacaactt aaataatact atagaaaatg ttacagcagc
    24541 tgaatcaaga ataagagata cagatgttgc ttcagaaatg gttaacttat ctaaaatgaa
    24601 tatattagtt caagcatcac agtcaatgct tgctcaagct aatcaacaac cacaaggagt
    24661 tttacaatta ttaggataaa ataattttgt atattggata tagaagaaag ggtaaggctt
    24721 tgctttactc ctttcttttt ttacaaatat acttttgaaa ggaaaattta tatgttactt
    24781 agtatagtta tgattgttaa aaatgaagaa aaaatacttg aaaaaacttt aaaatcatta
    24841 accacattaa gaaattctat agaaagtgaa cttataatag tcgatacagg ttctacagat
    24901 gatactataa aaatatctaa gaagtacacg gaaaaagtgt attttcataa ttggaataat
    24961 gatttttcta gtatgagaaa tatatcaata tcctatgcta aaggtgaatg gttacttata
    25021 ttagatgcag atgaaatact aattgattct tcaactattg taaaattttt taaagatggt
    25081 cttgataaaa aatttaattc tgcttcaata caattaaaaa atctttactc atatgataaa
    25141 aaattatatg gatattgttc agtgcttaga ctttttaaaa acgttgattt tagataccat
    25201 ggtaaagtac atgaacaacc aatatacaaa aaccctatat ttaataatgt tgctgatttt
    25261 gaacattatg gatatttgtt tgaagatgaa gaaataagat taaataaggt gaaaagaaat
    25321 gaaaaactat tatttcaaga gttaaaagag aatgataatt ctccatatac aaactatcaa
    25381 ttagggaaaa attttattat attaggaaaa tatcaggagg ctttatacta tcttgagaaa
    25441 tcttatgaat tatattctaa actagaattg gcacctggtt acttgataac aagccttgta
    25501 aaaacatatt tatatttagg aaaacataag aagtgtgaaa gattgtgctt aaagtatatt
    25561 aagaaagaca tgaataatat tgatatatat tattatattg cacaagcaca agtagattta
    25621 ggaaagtatg aaaatagtat agattcgtat aagagatata tttacttgtt agataattat
    25681 gaaataagta cacaagcaaa tagtttattt tcagacactg atactgtagg gcttagagat
    25741 gaggctatta ttacactgat aaagatttat tataagcttg aaaaatatga gtttgtaatt
    25801 tcagagtata ataacattga agatgtggaa aagaaaaaga atgtgtactt tagcttattt
    25861 atgtcactat ataaattgaa tagatttgaa gatataaaaa attattataa agaagtttca
    25921 aattctaaaa ttgaaaaaaa tagtttttat agaaatatag aagaagttat aaataatatt
    25981 aaagaggatg aaaaagagta tgtatataaa atcctttgtg acattgaagg taattatggc
    26041 attttaaatc aaataaggat aaataaatgt gtatctatag ataaatgtaa agaaatactc
    26101 aatgaagaaa aggaaataat ttatgcatat attataaaaa tagcttttga agagaagatt
    26161 gatttactag atatttttta tgaaatggaa tatgtatgga tagagaaata tctaaaatat
    26221 cttttagcat tagaaagaag ttttaattta gtactatata agtacataat tagtcgtcca
    26281 aatacatcag atatggaaaa gataagagta tataaagcta tatctaaagt agttttagaa
    26341 agtataagct taagtgatga aaaatataag gagatttttt atctttatat aatgtattct
    26401 tataagtaca taagatacat atatagcaat ttaagtgatt ttgaattaat aaaatttgtt
    26461 tgtaatgatt tagataagtt cactatagaa tttaagcatc tacttgatat taaaaataaa
    26521 gtaaataatg ctaatgaaaa attagagtat atacatagta tgaggaaatt actaaatgag
    26581 tatccttttt ataataaaat aataaaaata cttataggtg aattagaaga aaatataaaa
    26641 gaaagtgaag aatttaagga tttaaaagat agttttatta ttagtataga aaatatgatt
    26701 gaaactggca aaataaatga tgcgaaatta ttggtagatg actattcaaa aacatttgtg
    26761 gatgatataa agattttaaa tataaaggga attttataca tgtttgaaaa taagcttgat
    26821 aaagctgatt ttatgttaaa aaaggcatta tcattagatg tagaaaatga agatactata
    26881 tataatatag aatacttaaa aagtttaaga taagtcaaaa ataggagaat tgaagaatga
    26941 atataccaat agtggttaat gaaaaatcta aagacataat aaaagattgg caatatacat
    27001 gtacaaatga agatataaaa tttacaagca taataatatt gacatataat caaatagaat
    27061 atactaaatt atgtattgaa agtattagga aatttacccc aaagaataaa tatgaaataa
    27121 taataattga taataactct tctgatggaa cagttgagtg gttaaaggga caatctgata
    27181 taaaatctgt gtataataac aaaaacttag gatttccaaa aggatgcaat caaggtattg
    27241 agttagctac tggtgaaaac atattgttgt taaataatga tgtaatagtt actccaaatt
    27301 ggctctataa tttgaataaa gctttatggt ctcaagataa tataggtgct gtaggaccta
    27361 ttacgaatgc atgttcatat tatcagcaaa ttgatgttga ttataagtca attgaggaca
    27421 tgtttatatt tgcacacaaa ataaataaat ctaatattaa cctatggaaa tatagattga
    27481 agttagttgg attttgcatg ttaataaaaa aaactgttct ggataatgta ggggttttag
    27541 atgaattatt cacacctgga aattttgaag atgatgattt atcttttagg atgatagagt
    27601 caggatataa gttattatta tgccaggata cttttataca ccattttgga agtgtgtctt
    27661 ttaaagaaaa tcgtaataaa tatacagata taatgtatat taatagaaga aaatttgaaa
    27721 ctaagtgggg atttaatcca gaatatagtg gaatgataag aacagatttg atttctaaaa
    27781 tagatgatag tttggaggca aatattaatg ttttagaaat aggatgcggt ataggggcta
    27841 ctcttttaga aataaaaaat atatataaaa atgcatcaat atatggaata gaaattagtg
    27901 agtcagcagg taaacttgca aagtatagtg cagatgtact tatagcaaat atagaaaaag
    27961 taaaattgga ttataaaaaa gatttttttg attatataat attgggagat gtacttgaac
    28021 atttgattga cccatggaag atagtcaatg aattgaaaca atatctaaag aaggatggtt
    28081 atctggtatc tagtatacca aatattatgc atgtttctgt tttaagaaat cttgtgttag
    28141 gtaattttac atatgaagat tcaggaattt tggacaagac tcatttaagg ttttttacat
    28201 taaatgagat aacaaaaatg tttaatgata gtaattacat aattaatagt attacagcta
    28261 ctagtatgcc actttcggaa attgataata gcttcattga tacaatatgt aaggaaagct
    28321 gtaaagaaat gagacaacag tacgaatctt atcaatatat agtaaaagcc tctaaaatcc
    28381 tagatactaa aagatattct agtattagta tgattaattt aaaatacgct ttaatgagaa
    28441 ttgataatga attagatgta gatagtaatt taagttatat atttgataat tataatataa
    28501 ctgaaccatg ttttgttgat gatattgaat atctaattga tacatttgtg ataaataaag
    28561 caactctatt aaatagatta ggagttgagg catttaacag aggtctagga gattttagta
    28621 taaacatgtt tatgacagta ttagagatgg atagagatga tattgactca atatataata
    28681 taacaagtgt attatgtgaa ttagaagaat ttgatactgc ttataattta attaaaaata
    28741 gtagcgaaaa agtacagagt gaaagtgata tacaagagat actttgtttt attgagggga
    28801 agaaatatga ataatgataa gatatgtttt ataacctgtg taaatgatga aaggctttat
    28861 gaagaatgtc ttgtatatat aaatagccta gacattccta aaaactttag tatagaaaca
    28921 gtagctttaa gaggagccga ttctattgcc tctgcttata ataaagctat gagagaaaca
    28981 gattctaagt ataaggtata cttacatcaa gatactttta taattaataa aaatttcata
    29041 catgatattt tagagatatt taaaaaaaat acagatgtcg gaatgattgg tgtagctgga
    29101 gtaaaagata tgcctgtgaa tggaatttgg tgggattctg ataaaaaagc agggaaagta
    29161 tatgatagtc atacaggtaa aatggatatt tatgaattta gtaactttga agaatgtttt
    29221 aatgaagtta tggctataga tggattaatt atgattactc aatatgatgt attttggaga
    29281 gaggataagt ttgatgggtg gcatttttat gatttgtcac agagtatgga gtttagacaa
    29341 aataaataca aagttattgt accaaaacaa aataagcctt ggtgtatgca tgattctgga
    29401 gtagtaaata taaataatgg atatgatgaa tataggataa aatttgtaaa agagtacata
    29461 aatcttgatt tatatagaga aaatttttat gaatttgata agaagaaagc aagtaccata
    29521 cttaagagta ataagcatgt aatggattgt atgaaagagg agcttacaga acaattaaga
    29581 ttaaagaatt acaaggatgt tttaggtatt gtttctaaaa ttgctcattt ttctgttttt
    29641 aatcatactg gtttgtattc ttgtgaagaa tgtgagagag cattgctaga atgtgcaaat
    29701 aatttaccag aaatagaaaa tacgaaatta tttacaaaaa aagatttaaa aagaagaaaa
    29761 gtattacatg ttttaagtga aggatattca acaggaggtc atacaagact agttaaaaat
    29821 tggattgaaa ttgacaaaga ctctgttcat tcagttgtaa ctacatggca atcagataca
    29881 ctgccagact ggttattaaa agcaataaat gatagtggtg gcgaagttat atctttggat
    29941 ttaatttcag atgattattt aaaaagagct gtagctttga ggaaaatagc ttatgactgg
    30001 gctgatatag ttattttata tgttcatatg tgggatacaa tacctgttgt tgcttttgga
    30061 gttgatggga aaatacctat tttacatgtt aatcactatg atagtacttt ttggttaggg
    30121 acaagtattg cagatattgt cattgatttt agcgaagcta gtcaattaat tacattagat
    30181 aaaagaggca tagataaatc tgaaatatta ccaatgccat taaaagaaaa agaagtattt
    30241 tcaaaatctg aagttagaaa acgatataat ttagaggatg atgtgacaat aatattgact
    30301 attgctagtg aaagaaaata caatagtgta aatgatatta attactttaa aattgctaaa
    30361 acattgattg aaaatacaga taatacaatt attattgttg taggagttga tacagagaat
    30421 ctctattgga aaactttaag taacgttaca gataatagag ttatatgttt tggaacaaca
    30481 gcaaatatag acgattttta caagatttcg gatatatata tggagtcttt catggtagga
    30541 agtcatactt caaaactaga tgcaatttta tatgatttaa ttcctgttaa gtttaaaaat
    30601 gaagtttcac caatttttac aaatatgtgg aattttatag ataagtttga ttataagaat
    30661 ataaaagagg ttatagatat gataaaaagg tacaatacta gtgaatttga cagggatagg
    30721 ataaaagaaa aacaatcgga aataaaacat tttgttatag ataaacactg tattgaaata
    30781 aaaaactata tagacaaaat atataatgaa gctaatcatc ataaagttaa gtatgattta
    30841 tataaaaatg atgaaatcga ggactatcag ctttttgtag cattatatgg ctatatggta
    30901 aaaaataaaa acatgtcata ggtggtaaaa tgaacaatat tcatatcaat aaatttgaaa
    30961 aaagctgtga aagaaactgg gattattttg tagaaaataa atctataaat ggtacatttc
    31021 tgcaaactag aaatttcctt aattatcatg aggagggaaa attcactgac cactcattaa
    31081 taatatgtaa aggtgaagaa atactagcag tcgtacctgc atgtgaaact tatgaagatg
    31141 gtgaaagaat atttttttct catttaggga gtacttttgg tggtattata ataagaagtg
    31201 gttttaatga tattaggcat gtaaatgcca ttacaaataa gttagaaata tatttacggg
    31261 aacaaaattt cgataaagta tatttaaaaa atacatcaag aatattttgt aaagaggata
    31321 cttcattact agaatatttt ttatttaaga ataattacat gttttatgat gaattaagta
    31381 tgtttttaga attaagtaag tataaagaag atatattagc caattttaaa tacagtaaaa
    31441 gaagagattt taaatattct ttgaagaata atcttacctt taaaagactg tatagtgatg
    31501 aagatatagt ttgttttttt aatttacttt ctaattgttt agatagtaaa ggagctaaag
    31561 taatacattc agtagaagaa cttttggagt ttaaaaataa tagattaaaa gatattgtag
    31621 aattttatgc agtatatatt gatgacatta tgatttgtgg gagcatggta tttaactttc
    31681 aagaaagagt ttatcatact caatatctag catcaaattc gacatataat aaatattatc
    31741 caatgatatt tttatactat aatctaataa aagatgcttt agagagaaat tttaagtatc
    31801 tttcttttgg aattagcact ctaaatagag gcaaatactt agacgagggt ttagctacat
    31861 ttaaagaagg ttttggttca agtgcaattt gtaacagaac atactataaa aatataaata
    31921 agtgagaatc atgttttata gagtttttag aatatagtaa atatctagta ctctaacatt
    31981 acataaaaat aattagttta taaagaaggt ttgtatatga aaagattaat gattttaggc
    32041 tcaattaagt attttgagaa tattgttata tcagctaaaa aagagggaat atatacgatt
    32101 gtttgtgata atagaattga tactcctgcg aaaaagattt gtgatgaagc aatagatgtt
    32161 gatgtatttg attttgataa aataaaaagt atagctatag ataaaaagat agatggaatt
    32221 ttaactggat ttacagattc tttaatgaaa ccatatgttt atattgcaaa tgaactaaat
    32281 ttaccatgtg tgatttcttg caatcaactt gaatctgtaa ctaacaaggc tattatgaaa
    32341 aaatatttta aaaataatgc aatacctaca actgattact gtattattga aagtctaaaa
    32401 gatttagaaa aaatacaagg attaaatttt ccacttgtaa taaaacctgt tgatggttat
    32461 ggttcaagag gtgtatattt tgtatcaaat atagaagaat taatagaaaa atttgaatat
    32521 tctaaggaag cctcacaaga tggacaagta ataattgagg aattttatga aagtgaggaa
    32581 atacaaggac tagcttgggt tcataatgga gagtcacatg tattttatat tggagataga
    32641 gagcttgtaa acatacatac aggaagagct ggaaaaccag atagattact ttatccatca
    32701 aagtattgtt ttcaatatga ggaggaaata aaatctatat atcaaaatat aacacaggct
    32761 tttgatataa aaaatggacc tctttatgta caaatgttag tgggagcata tggaataaag
    32821 gtatctgaag taatgccaag attacctgga ggttgtgact atatggctat ttctcaaata
    32881 acagatttag acataggaag tttatttgca aactttagtg tgaataatga agtaaatttt
    32941 gatgaaataa agaaatataa tatgcatcta tcaaaatgtg tatatgcatt accaatatac
    33001 ataagacccg ggacaataaa aaagataaat aatatagata aaatagaaaa cttagattat
    33061 gtcactcaat ttctattact agtaaaagaa ggagatacaa tagaattata tgaagatgag
    33121 agacaggatt gtggaagagt ttatggaata gcaaataata tatttgatgc aaatataaaa
    33181 aagaaatgta ttcatgaaat gatagaaata cttgatgaaa ataatgaaaa tatgatagaa
    33241 aatttttaaa taagggagta tgatagatat gaagctaata actcatgaac aaattatgtc
    33301 attaaacatt tctcagaaac aaggatatga gtggataaaa gaatctttct taacaaaaaa
    33361 agattgtcaa ttaccaccaa aaataagcat ggtagaagaa ggacatgtat tttacaatgt
    33421 aatgccatcc ctaaatcata atcatatgat tggtggaata aaaatggtta caagattgcc
    33481 agggagaaat ccaagtttaa actcagagat atttctatac aatatggaag atgctaaatt
    33541 aaaggctatt ttggatggta attatattac agcttttagg actgctgctg ttgctgcctt
    33601 ggctatcgat acatttgcta aaaaagattt taatactatt gctattatgg gtttaggtgt
    33661 aactgcttct gctataatga atatgctttc atatactctt aaagataaaa atataaatat
    33721 aaagttactt aaatataaag accaagcaga gttgtttgta aaaaagtatg aaaaaaatga
    33781 acaatttagt tttgaaatat gtgatacaca tgaagaactt attgtagatt cagatgtagt
    33841 tatttctact gtgacatata cagatgaata tttatcagaa tttaaatggt ttaaaaaagg
    33901 tgctttatta ttacctgtac atgttatggg atttggaaat gttgattatc tatttgataa
    33961 agtatattta gatgattata atcatttgaa acatctagaa aatatagata gatataaata
    34021 ttcttgtgaa ttttatgatg ttttaagtaa acaagctaaa ggaagagaaa atgatgatga
    34081 gagaataata tcttacaata taggtttgtc aatacatgat atattatttg caaataaaat
    34141 ttacgaattg tgtgaaacac taagctgtga agaaatagaa ttaaaagaac caaaagaaaa
    34201 atattggata tagtttattt cattcatatt tacaatagtt tattgtaaat atgaatatga
    34261 agtaatatat tgttagaaaa ttagcatgaa taggatagat aaatttatgg ataaatataa
    34321 tttaattagg ttcgtatcta ttggaaacga atatatagga aatttagttg cattagaatc
    34381 aaataaagta ataccttttg atataaagag agtttattac atatatgatg taccaaacaa
    34441 tgtaaagaga ggatttcatg cacacaaaaa cttggaacaa gtactagttt gcctaaatgg
    34501 aagtgtgaag ataaaatgtt atgatggtgt aaaagagact atatttaatt tacataaaaa
    34561 tgatgttgga ttatatatag gaacgggagt gtggcatgag atgtttgatt ttgatgaaaa
    34621 aactgtttta atttctatag catctgaaca ttataatgaa gaagattata ttagagacta
    34681 taaagaattt ttaaagtatt taaataatgt gtagttatta tacaattagt gatgtatttt
    34741 cttaattaaa taaatttaag atagccacat agagaataga tagattagga tgtgaattta
    34801 ttgttggtta aatttaataa tttattaaaa cagttccaaa ctcataaaga agaatatgaa
    34861 cagaagatta aggatgtaat ggaaagtgga tattatatcc ttggaaatga agtaaagcat
    34921 tttgaagaag aatttgctaa ctatgtaggt agcaaatatt gtgttggttt agccagtggt
    34981 ttagatgcac tgataattgc ttttagagca ttaggaataa gcaaaggtga tgaagtaatc
    35041 gtgcaatcaa atacatatat tgctagtgtt atgggaatca gtataaatgg agcaacacca
    35101 gtatttgtag aaccaaatga atattataat atagattcat ctaagataga agaaaagata
    35161 aatagcaaaa caaaagctat attagtagtg catttatatg gtcaagcaag taatatgaaa
    35221 gatatacaag aattatgtga taagtataac ttgtatttaa ttgaagattg tgctcaatct
    35281 catggagcta aatatgatgg aaaaatgaca ggaacatttg gtgatatagg ttgttttagt
    35341 ttttatccaa gtaaaaattt gggtgcattt ggagatgcag gagctatagt aactagtaat
    35401 gaggaaatag ctgataaagt gaaaattctt agaaactatg gctcagaaaa gcgatattat
    35461 aataaatttg tcggatataa ttcaagattg gacgaaattc aggcaggttt attaagagta
    35521 aaacttaaat atctaaatga aattgaaatt gaaaaatcaa aaatcgtaga tagatatctt
    35581 aatgaaatta ataatcccaa agtcatactt ccaaagatag aaaataattg tactcatgta
    35641 tggcatttat ttgttttaca agttgaaaaa cgagatacat ttatggaata cttaaagaaa
    35701 agtggtatag agtctataat acattatccg ataccaccac atctatctga agcatatgct
    35761 tatttgggat ataaaaaggg gagttttaat atagctgaga actattcaga taaagttgtt
    35821 agtatacctt tatttattgg tatggaagat gaagaagtta gttatataat taaaattata
    35881 aat
//
LOCUS       DAYAOX010000015        34382 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.015, whole genome shotgun sequence.
ACCESSION   DAYAOX010000015 DAYAOX010000000
VERSION     DAYAOX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.015"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(64..981)
                     /locus_tag="ACGI57_000622"
     CDS             complement(64..981)
                     /locus_tag="ACGI57_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFG9715709.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            1458..2621
                     /locus_tag="ACGI57_000623"
     CDS             1458..2621
                     /locus_tag="ACGI57_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9715710.1"
                     /translation="MFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKLSR
                     FIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNPYD
                     YLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKNDEN
                     NYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVIDRK
                     DEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPKSF
                     IDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFKEK
                     YNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2670..3521
                     /locus_tag="ACGI57_000624"
     CDS             2670..3521
                     /locus_tag="ACGI57_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFG9715711.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            3847..5748
                     /locus_tag="ACGI57_000625"
     CDS             3847..5748
                     /locus_tag="ACGI57_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9715712.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            5751..6794
                     /gene="ypdE"
                     /locus_tag="ACGI57_000626"
     CDS             5751..6794
                     /gene="ypdE"
                     /locus_tag="ACGI57_000626"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFG9715713.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            6809..7114
                     /locus_tag="ACGI57_000627"
     CDS             6809..7114
                     /locus_tag="ACGI57_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9715714.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            7211..7546
                     /locus_tag="ACGI57_000628"
     CDS             7211..7546
                     /locus_tag="ACGI57_000628"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9715715.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            7578..8900
                     /locus_tag="ACGI57_000629"
     CDS             7578..8900
                     /locus_tag="ACGI57_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9715716.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEGLLDDLGLDF"
     gene            8910..9980
                     /locus_tag="ACGI57_000630"
     CDS             8910..9980
                     /locus_tag="ACGI57_000630"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFG9715717.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            9985..11172
                     /locus_tag="ACGI57_000631"
     CDS             9985..11172
                     /locus_tag="ACGI57_000631"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9715718.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(11369..12241)
                     /locus_tag="ACGI57_000632"
     CDS             complement(11369..12241)
                     /locus_tag="ACGI57_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFG9715719.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(12635..12826)
                     /locus_tag="ACGI57_000633"
     CDS             complement(12635..12826)
                     /locus_tag="ACGI57_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715720.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            13046..14092
                     /gene="cobT"
                     /locus_tag="ACGI57_000634"
     CDS             13046..14092
                     /gene="cobT"
                     /locus_tag="ACGI57_000634"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFG9715721.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            14124..14684
                     /gene="cobU"
                     /locus_tag="ACGI57_000635"
     CDS             14124..14684
                     /gene="cobU"
                     /locus_tag="ACGI57_000635"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFG9715722.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            14694..15467
                     /gene="cobS"
                     /locus_tag="ACGI57_000636"
     CDS             14694..15467
                     /gene="cobS"
                     /locus_tag="ACGI57_000636"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFG9715723.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            15487..16101
                     /locus_tag="ACGI57_000637"
     CDS             15487..16101
                     /locus_tag="ACGI57_000637"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFG9715724.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      16168..16347
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            16462..17976
                     /locus_tag="ACGI57_000638"
     CDS             16462..17976
                     /locus_tag="ACGI57_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HFG9715725.1"
                     /translation="MGKKIMLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMAL
                     NSFITKEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSS
                     KYHDYKLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPV
                     IIVGDIDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKV
                     PVIGVVPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVS
                     IRYVDYGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQ
                     MLGKKLKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGK
                     KVKGYEIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEIS
                     FTRAILNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            18015..19391
                     /locus_tag="ACGI57_000639"
     CDS             18015..19391
                     /locus_tag="ACGI57_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HFG9715726.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            19392..20363
                     /gene="cbiB"
                     /locus_tag="ACGI57_000640"
     CDS             19392..20363
                     /gene="cbiB"
                     /locus_tag="ACGI57_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HFG9715727.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            20395..21465
                     /locus_tag="ACGI57_000641"
     CDS             20395..21465
                     /locus_tag="ACGI57_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9715728.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            21467..22354
                     /locus_tag="ACGI57_000642"
     CDS             21467..22354
                     /locus_tag="ACGI57_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFG9715729.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            22342..22974
                     /locus_tag="ACGI57_000643"
     CDS             22342..22974
                     /locus_tag="ACGI57_000643"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HFG9715730.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            22983..24209
                     /gene="cbiD"
                     /locus_tag="ACGI57_000644"
     CDS             22983..24209
                     /gene="cbiD"
                     /locus_tag="ACGI57_000644"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HFG9715731.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDNLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            24206..24814
                     /locus_tag="ACGI57_000645"
     CDS             24206..24814
                     /locus_tag="ACGI57_000645"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HFG9715732.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            24804..25379
                     /locus_tag="ACGI57_000646"
     CDS             24804..25379
                     /locus_tag="ACGI57_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HFG9715733.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            25372..26124
                     /locus_tag="ACGI57_000647"
     CDS             25372..26124
                     /locus_tag="ACGI57_000647"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HFG9715734.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            26108..27229
                     /gene="cbiG"
                     /locus_tag="ACGI57_000648"
     CDS             26108..27229
                     /gene="cbiG"
                     /locus_tag="ACGI57_000648"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HFG9715735.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            27226..27951
                     /gene="cobJ"
                     /locus_tag="ACGI57_000649"
     CDS             27226..27951
                     /gene="cobJ"
                     /locus_tag="ACGI57_000649"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HFG9715736.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            27948..28700
                     /gene="cobK"
                     /locus_tag="ACGI57_000650"
     CDS             27948..28700
                     /gene="cobK"
                     /locus_tag="ACGI57_000650"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HFG9715737.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            28719..30197
                     /locus_tag="ACGI57_000651"
     CDS             28719..30197
                     /locus_tag="ACGI57_000651"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HFG9715738.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            30227..31132
                     /gene="hemC"
                     /locus_tag="ACGI57_000652"
     CDS             30227..31132
                     /gene="hemC"
                     /locus_tag="ACGI57_000652"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFG9715739.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            31122..32621
                     /gene="cobA"
                     /locus_tag="ACGI57_000653"
     CDS             31122..32621
                     /gene="cobA"
                     /locus_tag="ACGI57_000653"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFG9715740.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            32638..33603
                     /gene="hemB"
                     /locus_tag="ACGI57_000654"
     CDS             32638..33603
                     /gene="hemB"
                     /locus_tag="ACGI57_000654"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFG9715741.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            33756..34334
                     /locus_tag="ACGI57_000655"
     CDS             33756..34334
                     /locus_tag="ACGI57_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HFG9715742.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        13845 a         3498 c         6250 g        10789 t
ORIGIN      
        1 atatagtctt atataaaaat aggttagaaa aattcatttt taacctattt ttataataaa
       61 tatctatttt actgaattta taattttctc tagtaatctt tgagctttga tacactcttc
      121 tccattaata gctggtttta tattattttc tatacaatca ataaaatgat ttgatatatc
      181 ctcaaaaccc ttttgtttta atatatttac ccatgcacca ctttgcttta aagttaggtt
      241 tccaccttct tctatttcca gtacattaag attcttaact ctttgaatct taccctttga
      301 aagaatttca acttgttcta actttgttcc agaatctcta tgcatagagg tgtttataga
      361 gaaattttta cctttaagtt tatgacttac aaatataaga ttcttattgt ctgttaaaaa
      421 taaatcctct ccactaattt caacatcttc atttgctaac cataaagcag tgtcaataac
      481 atgtatatag tcatctataa gagtactgtc gaatctaaca tttcttaagg aatttaatcc
      541 atgtttacat atattgatag atacaatttc tgtagcatta tttttaattt ctttgtacat
      601 aggacagaat ctacggttga atccaaccat taaatttaag ttttttttcg tacttaactc
      661 gataagttct tctccttgac ttactgttga agcaagaggt ttgtctacat atacatgaac
      721 acctaaattt aataatattt ttataatttc gtagtgtgtt tctgtagagc tatgtagaaa
      781 aatacaatca cattttttag ctagggattc aatagaatcg aatggcatga ttctataatc
      841 actacagact ttttcccttt taactttatt tggagtaaaa gctcctacaa actcaaatct
      901 ctcagactta gttaatatag gtaaatatgc cttttgagct atggagccaa gacctatcat
      961 tcccattttt atatttttca taatactttc tcctaaggtt tttatattta cagaattgat
     1021 tataattcag acacataaat ttgtaaactt tgtatcaaaa aataaattta aagcttatat
     1081 aagtaaatta gcgaaataag aaatattgat aaagtaattt tgtaggatat ccataaaatt
     1141 gattgatata aatattttat gtattgacaa aaaattcaaa ttaatttata tttggattag
     1201 tggtaattac cactttggtc tgaacaagct tttatcgtaa aataaaaata ctaaagtata
     1261 tagagtatga ttttattaaa tttttaaaga taaactataa aattaattaa aatctatagt
     1321 gtatatttta tagtgagttt aagtagtact aaaaaatcta agcaaaaaat tataaaagag
     1381 tatatttttt tatagtttaa agtggttata acctcattac caaatgtaaa cgttacaagt
     1441 atgaaaggag aatgaaaatg tttaaattag aagaacaaaa actaaaagat ttaggtgcaa
     1501 taataacaac taatgaaata aaacaacagc ctgaattatg gttagaaact tatgaaatat
     1561 ataaatctaa caaagaaaaa ttaagtagat tcatagatac aataagtaat aatcatgggc
     1621 agtttagagt tatatttaca ggtgctggaa cttcagctta cataggaaat tcaatacttc
     1681 cttaccttaa aaataaaaat gacataagaa aatacatatt tgaagcaata cctactacgg
     1741 atatagtttc aaacccatat gattacttaa aaaaagatat accaacatta ttaatttcat
     1801 ttgcaagaag tggaaatagt cctgaaagtt tagcagcttt aaatttaggg aataagattg
     1861 tagataattt ttatcattta gctataactt gtaatccaga aggagaactg gctaagatga
     1921 ctaaaaacga cgaaaataat tacttgttat taatgccatc aaaatcaaat gatgaaggct
     1981 ttgccatgac aggaagtttt tcgtgtatga tgttatctgc tatgttaata ttcgatagtt
     2041 tagaagatga tgtagaaaag tcatatataa atgcaataat tgaaatggga agaaatgtta
     2101 tagatagaaa agatgaaata catgagttaa taaataaaga ttttgataga gttgtatatt
     2161 taggttcagg aggattaggt ggtttgacac aagaagcaca actaaaactt ttagaactta
     2221 cagcaggaaa aatatctaca gtgtatgatt ctcctatggg gtttagacac ggaccaaaat
     2281 catttattga tgaaaataca cttgtatttg aatttgtatc taactgttta tatacaagaa
     2341 aatatgattt agatgtttta gaggagataa aaagggataa aattgctaaa ttcacttgtg
     2401 cagtgtcagt agaaaatgaa aataacttct caggaacaaa gtttgagttt aaagaaaagt
     2461 ataataaatt accagatgtg tatcttgcaa tgccatacat attgtttgca caaactatag
     2521 cattgtttgt atcagtaaag gttggaaata agccagatac tccatcagct acaggaacag
     2581 taaatcgagt agtaaaaggt gtaacaatat atgaatatta aaagataaga ttaaaatatt
     2641 ataactaaat aatttgggag ggtatcaatg tggcattagt aacaacaaca tcaatgctta
     2701 aaaaggcaca agatgaaggg tatgcagtag gagcatttaa tgtagaaaat atggaaatgg
     2761 taattgctgt tatagaagct gctgaggagt taaattctcc agtaatatta caaactactc
     2821 catctactgt aaaatatgca ggtcttgatt attatctagc aaatgcaaag gttgctgcag
     2881 aaagagtaaa tatacctgta gcaatgcatc tggaccatgg ttcaagtttt gaccttgcaa
     2941 tgaaagcttt aagaagtgga tatacatcaa taatgataga tggttctcat gaaagctttg
     3001 aaaataatat atctatatct aaatcagtag taaaagcatg tagtccttct cttataccag
     3061 tggaagcaga acttgggaaa gttggtggaa aagaagatga tttagatggc gggaatagta
     3121 atgattatac aaatccaaaa gaggcaaaag agtttgtcga tagaacagga atatcttcat
     3181 tggctattgc tataggtact gcacatggat tgtataaggg tgaacctaaa atagatttag
     3241 ataggttatc agaaattaaa aatgtagttt caataccttt agtattgcat ggaggttcag
     3301 gaatacatga tgatattata aaagaagcta taaaaagagg tattgcaaaa gttaactatg
     3361 caactgaatt aagaattgca tatagtaaag gggttaaaaa agttttagat gaagattctg
     3421 aggttataga ccctaaaaag tatggattag cagggcttca atcagtaaag gattttgtga
     3481 aagaaaagat taaagtgtgt gggtctgtaa atagagtata aaaataaaat atcctaaatt
     3541 tattttagat aagtttagga tatttattat atgaaaaatt tatattaatt atatgtaaat
     3601 ggtgtatttt tttcatttaa ttgatgaaaa atatcagctt ttattggtaa agtattacat
     3661 atataattaa aatatactaa aaattgaact atatatttta ttattataaa atatttatat
     3721 taaattgtgg aattaaaata tactaggata taaaattctt aaataaaaag aaaaggttgt
     3781 ttttataaaa aatatttttt ataccacttt aaaaaacaca ataataacac tgagggaggt
     3841 ggggatatga gagaacgcat tattcttgaa acattaaaaa atagtagtgg ttatctaaac
     3901 atagattatt ttgcagataa gttgggggta tctactcgaa ctatcagaaa tgaaataaaa
     3961 aagatagaaa ctataaaaga atgtaatggt tttaaattag aatataagtc taaattagga
     4021 tatattctaa atattaaaga ccaagataaa ttcgagcatt atctaagaaa cttaccctca
     4081 gatttagttg aaaatccaga acaacggttg gagtctataa tagtagagct tttagtaaat
     4141 gaaggttata aaactataga acagttaagt aaaaaatttc tagtaagttc atcccaaata
     4201 aaaaatgatt taaaaaagat agatgaaaag ataaaggata cagaattaaa actagagcga
     4261 aaggctcatt atggaattaa aatagaaggt tctataaaag aaattcaaag tatattggtg
     4321 gatagttatt ttagagggaa tagaaatata acagagtaca ggaataagtt tatagataat
     4381 ttaaaattag ctaatattag aagtacaata aaaaatgtac ttaatgaaca tgatttagaa
     4441 gcaaatttaa cagaacttga ggaaatatta gcacaaatta ttatattgta tataagagtt
     4501 agtatgagag tagtgggaaa tgctagcgat ttaaaattat atacagaaga tttaattata
     4561 gataaattat tagataaaat ttttagagat aaaaaatata accttaatta tgatgaaaag
     4621 tattatttaa aggagcttat aaaactaaag actaaagata agaaagccac tataaagaat
     4681 attgataaga ataaacttca agatattatg ttcgaatttt ttagagagat agataaaaaa
     4741 tataactcta attttttaga agataaagag ttttttaact tattttactt acatattgca
     4801 tgtttaatag aacgtataaa gaagaaccac aagataataa atcctttttc agttaaaata
     4861 agtcaacagt atccaactgt ttttaattta tctattcaat tttcaaaaat catagaaaat
     4921 gagtatcata taaaaataag tcaagatgaa atagggttta tagctactca cattgctgtt
     4981 ccttttgaaa agagagaaga agctaatttt aataaaaaat ataaaatagc aataatatgc
     5041 tcttctggtg gaggtagtgc atttttgata aatttaagac ttaaagaaat ttttccaaat
     5101 gctgagataa gaaatttttc attactagaa gaaaaagctg taatagaatt tgcaccagat
     5161 ttgatttttt caataacaaa tcttttattt gaaataaatg ctccagttat acttataaat
     5221 gaaattttag acgaattgga ttatttagat attaaagaaa gtgttagatt tgcagaccat
     5281 ataggaaata tatcaagccc aaaacaatat atattaggtc tatttgataa gaatcacttt
     5341 aggtgtataa aagataaagc taagtataag gatatccttg atagtatgtc aaaatgtata
     5401 gttgacgagg gagcttgttc tccaacctat cccaaagatg tatgggaaag agaaagttat
     5461 ttgagtacta tttatacaaa tggggtggct atacctcatc caatcgagat gacaggaaac
     5521 aaaaatataa tatcagtagc tttgatacaa actgatatta tttatgaaaa tagggtgcct
     5581 aaaattattt tcatgatttc attaataaag ggaaatttag aacttcataa gcaaatatct
     5641 aaatatttaa gtaagattat ggcaaataag gatatggtag acatgctaaa taagtcacaa
     5701 tcttatgaag agtttatgta taagttaaaa atctatctgg gaggttagac atgaatatag
     5761 agttttttaa gaggttgtca gaagcagatg gtatagcatc taatgaagaa gaggttagac
     5821 atgttttatt ggaagaacta aaagcttata gtgataaaat tatctgtgat gggctaggga
     5881 gtatcatctt ttctaaaata aaagatgaga gtgcaccaaa cgtaatgatt tgtgctcata
     5941 tggatgaagt tggatttatg gtgagaagta tagacaaact tggtatgatt cacttaataa
     6001 caataggagg agtaaaacct cttgcacagt ttgtacaaaa agttagaatt acaacaaaag
     6061 aagggaagaa aatccccgct gtcataaatg ctacatataa taatggtaag gctgaaaata
     6121 tatatgcaga tattggagca tatacagaag aagatgtata taacctaggg attaatgttg
     6181 gggatatggt aacatataca acaagctttg aggaatttac tttaccagac cgattagtgg
     6241 gaaaggcttt tgatgatagg ataggatgtt ttgtgatggg agaagtttta aaagaactta
     6301 ggaaagaaaa tctaaattgt aatattcact ttgcggctac aagtagtgaa gaggttggaa
     6361 taaggggagc aaagacatca acacagttaa taaatccaga tatagtattt gtaatagatg
     6421 tagcatgtgc taaaaatgag tttgtgagag actatacaaa taagaaacaa attgataaag
     6481 gtgtaatgct aatgcataga gataggactc tagtccctag tagaaaaatg atagattatg
     6541 taatggaaat tgctgataaa aataatatac cacttcaaca tgatatgttt gaatcaggtg
     6601 ggactgatgg aggagaagca catctcgtaa atgaagggaa accttgtgta gtaacttgtg
     6661 taccagttag atatggtcat tgtgcatttt caatagtaag taataaagat ttggagaaca
     6721 ttataagatt gtatacacag ttaattttaa attttgatga agaacaatat aaacatataa
     6781 gaaattttat ctaaaggaga ataagaaaat gtcaacagaa gtacaaataa tggaacttat
     6841 atcaactgca ggagagagta aatcgaaagc ttttgaggct ttaaaaaaag tgaagtcaaa
     6901 agattttaaa ggagcaaaat tgttaataga ggaggctcgt aaaatagata ttgaagctca
     6961 caggatacaa acagaattaa taactcaaga tttaaatgaa gatgttgaaa gtccagcaat
     7021 gagtctttta atggtacatg ctcaagacca ctatatgact tctcagctag caaaagactt
     7081 gatagaggct ttgatatatt gtcttgataa gtaattgtaa tatagaaata gattatttat
     7141 gtaaaaattc ttttaaaagt ttagatgctt ttaagagata ataaaaatac aaactttagg
     7201 aggaattatt atgaaaatat tattatgttg tgcaggtgga ttttctacta atatgttaat
     7261 gcaaaatatg aaaaaggtgg taaaagagag tgctaaactc aatgaaaaag attttgagtt
     7321 tacagctata ccagcagatt caatagaaag tgaagttgat gattgggata tagtacttat
     7381 aggtcctcaa atagctcaca aaatagactt cttaaaagga atattagagc caaaaaataa
     7441 gccttttgca gtaatagata aagatgtata tggaaaaatg gatggagcta cagttatgaa
     7501 gttggcatta ataacttata agaaacatca attagctcaa ggataataaa aataatgagg
     7561 gaaaagggag attagatatg ttttcaaaat ttgaagcatt tatgaataga gtatttatgc
     7621 ctttagctca taaggttgac aatcaacgtc accttggttc tataaaggct ggtatggttg
     7681 ctatgacacc attcacaata ttaggaagta tatttgcaat acttccagca cttccaaata
     7741 tgcttggaga aaataatcca ataagcaaat tcatacttag caattcagca atattggatt
     7801 taccagtaag tctttccata ggtttaatag gattatatgc ttgtatgtgt attgctttta
     7861 atctggggaa ctactataaa ttatatatac caggttgtgt tgcactatca actttttctt
     7921 ttttattcct agtagcctca tttacaccag atggagcttt agaaatagga aatttaggag
     7981 ctaaagggct atttacaggt atgatagcag ggcttggttc tgtggagtta tataattttt
     8041 gtaataagaa gaatatcaca ataaaaatgc cagaaaatgt acctgatttt gtatctaggt
     8101 cttttgaatt aatacctact actataatag tttgtggaac ttttatagtt gctcgtttta
     8161 tttcattaaa agtatttgga gaattacctc cacagatact tactcaattt ttatctccat
     8221 tagtaggaag tatggataac ccatggtcag ttcttgcact taactttgct atatgtacta
     8281 tattcttttt tggaatacat tcatcagtat ttagtcctat aactagacct ataatgatta
     8341 catttatagc tgaaaacata gcagctatgc aggctggaga aacaatacct catttcttta
     8401 cagcaggagc ttcaagtgca ttctttggat ttacaggatg tggaattagt ataggttgtg
     8461 taattgcatg tatgttatca aaaaatgctt cctataagaa gattggacgt gtatcattat
     8521 tccctgcatt atttgggata aatgaaccaa tattatttgg agcaccgata atattaaatc
     8581 caataatgtt tataccacat gtatttggtg gagctataat aggtacacta ccaatgtttt
     8641 ttatgcactg gggatggtta gcaaagccag tatttgaccc tccatatgta ggggtattct
     8701 tagaaggatt tttaactaat ggagattggc attctatatt agcaaatgca ttgcaattag
     8761 tattagcagt tttattatat tggccattct ttaaaataat ggaacgtcaa caaaataata
     8821 aaaaacaaaa agaagaaact aagaatatat tctctaaaga agaagaaggc ttattggatg
     8881 atttaggatt ggatttctaa tattttgtta tgaataatac aatggagaga ttagtatcct
     8941 taatctataa aagaggtgtt gacgctcttt tgattaaagc aaaaaatact aaaagatata
     9001 taggagcttt atcaggtagt ggtgtatatg tgcttgttac taaggataaa aattatcaaa
     9061 ttctagatgg cagatatatt gatgaagcag ataaaaaaac gactggtttc ataaaaagga
     9121 ttgtatctca aggttcttat ataccaacta taattgagtt attaaaagaa ttaaatataa
     9181 aaaagttagc aatagaaaac caagctatga gtatacaaga gtatctttca ctaaaaaatg
     9241 aaggttttga aatcaattta ataacaaatg aattatggaa gctaagggct ataaaatcta
     9301 aagaagaaat taaacttatt aaaaaggctt gtgagattac agatgaagta tttgatgagg
     9361 ttatttctga aattaaaaaa gatatgactg agctagaagt atcagctcta attcagtatc
     9421 atgcattaaa aaaaggtgca agtggaatgt cttttgaaac aatagtggta tcaggagaaa
     9481 gaggtgcaat gccacatgga agacctacaa acaaaaaatt gaaagtgaat gaagctataa
     9541 caatagattt tggtgttgtt tatcaagggt atcaatcaga catgactcgt acaatatcta
     9601 taggcaaacc tcctaaaatt attaaagaga tatatgatgt agtattggaa gcacaattat
     9661 ctgcaataga gtcaataaag gaaggaacta gggcaagtga tgttgacaaa gtggctagaa
     9721 aaattataga taaacatgga tttggcgaat attttaatca tggactgggt catggtatag
     9781 gtcttggaga tggtgaagtt cccactttaa atccaaatag tgaagatata ttagtggaag
     9841 gaatggtaat gtcttgtgag ccaggtattt atattccaaa tgtaggggga gttagaatag
     9901 aagatgatat tgttataata gatggaaaag gtgttccact caataagaca tccaaagagt
     9961 ttataatatt aggagagtaa aattatgaaa tataatttta ataaagtatt aaatcgtaaa
    10021 gaaggaaatt gtagaaaatg gagcaaccat gtgattaaag aaaaatttgg cttaaatgaa
    10081 gatgcaattc ctatggattt agctgatatc gattttgagt gtgctcctgc tataaaagaa
    10141 tctatcttaa aaagagcatc agtaggagat tatagttata cttttatagg agatgacttc
    10201 tatgatgcta ttataaattg gaataaaaga agatttgatg tatatataga aaaggattgg
    10261 ataaaattaa cttttggtac tgtttcaacc cttcattata tagtccaagc atatactaaa
    10321 gaaggagagg gtgtattgat aaatacacct gcttatgacc cttttgcaga ggctgtagta
    10381 aataataata gaaagttgtg ttgtagctca ttaaaattag ataacaatag atattattta
    10441 gatttagagg atatggaaaa tcaaatgaaa tacgagaata taagattgct tatattctgt
    10501 agtccacaaa atccatcagg tcgtatatgg acaaaagaag aactatatca agtgtcagag
    10561 ttatgtttaa agtataatgt aattttggta agtgatgaaa ttcatagaga tataatattt
    10621 aaagattata gatttgtatc tttgtggaat gctcatccag aaatttataa aaatagtata
    10681 atctgtgtat ctcctaataa aggcttcaac ttaggtggat taaaaacatc atatgtatta
    10741 ataagagatg taaaaattag ggagacttta ttagaaaggc taaaaagtaa ttcaataact
    10801 tctccaaatg tatttgcaat accagcaata gttgcagcat ataatgaaag cgaagaatgg
    10861 ctagatgcta tgactagtta tgtagaggag aattttgaaa tagtttatga tttctttgaa
    10921 actaatatac caaaagctaa agtgatgaag tcagactctt cttttttagc ctggatagat
    10981 gtaagagaag tatttagaaa tgaagaagaa tctaaagaat tttttagaca tgcaaactta
    11041 actatggtag ttggaagtta ttttgtgaaa gatggagatg gatttattcg tattaatgtg
    11101 gggtgtccta gagaaacatt aaatgaggct ctaaatagaa tcaaaaaaac gtatataagt
    11161 atgtattgtt aaatcataaa aaaagtatct atggtttata taaaaacaca ttatttagtt
    11221 atcaaatatt actggaattt tgtattccag taatattttt tgattaagga atattattga
    11281 ggaatattaa ataatattat ttcataccct tatattaact tatataaaaa tattttatta
    11341 ttttgggaac gggttagcta acctttatct agtgtatatt tatattgtaa gaagcagtaa
    11401 aaacttttcc tttactaagt ttattaataa acttcttatc tttataaatt ttgttagaat
    11461 ttatactatc agcaagacca taccaaggtt ctatacataa aaaaggagca gtagaattag
    11521 tttcactata ataaggagtc caaataccaa ctagagggaa atcttccata gatatagtta
    11581 catatttact accatcatta ttacatatag acacttcatc tatgttggtg tatatcaaag
    11641 catcattctt gaatacttct ggatttaact gtaagttttt taattttcca ataggtatta
    11701 tatcatctgt aaaagaacca tttaaattga ttttttctac atcattttta cttttaaact
    11761 caaggtagtg tttagaaaaa tcattttgtt cgtaaaaagg aaggttgaat gcaggatgtc
    11821 cacctataga aaagaatata tctttagaat cagtattttt tacaatccat tctacctcaa
    11881 tactattatc atttaatgta tagtttatga ttagctcaaa agaatatgga tacttcttta
    11941 aagtagattc attatctgtt aatttataag ttacaaatgt atctccttta tctacaatct
    12001 caaagtccat atctctagca aacccatgtt gagtcatgct atataaattt tcttctatta
    12061 ttgtttcatt atcaaaaagt ttacctacta tagggaacaa tataggtgat tgtctacccc
    12121 aatatttttt attaccatcc caaagaatgt ttttgtcgta ttttttagaa aatattttag
    12181 ttagttcagc accaaaactg cttgattcga taattaaatt ttcattttgt aatatattca
    12241 tttaaaatct cctttattat attttatgag tatattatac tataaagtat aaagattatt
    12301 aaattttatt tgatattata tttgaaaggt agattttagt atattttgaa aattttgttt
    12361 tgaaaatttg ggattattta agaaattttg tattataaat agaaggaaat agataaattg
    12421 tatagaataa aatatattaa gataagttaa taatgttaaa tgggaagtga gtttatggaa
    12481 aaaccaaata caaaagttgt taactttcaa gagtataaag acagaaaaaa ccaaagagaa
    12541 gtaaatagag acgaactttt aaagcttata aaaaaaattg tgacttcaaa atagggagtt
    12601 gttaaactcc ctattttaat gggtttcata ataattaaga cattctaagt ccatctatat
    12661 aatctttgaa ttgtgagaaa tctgattcac tacttaattc tatggtttct gaaccttccg
    12721 tataaaaatt cttaagatag atagtataat tattatcaac acaatcatga taaatacatc
    12781 tatatccatc ttcatacaaa ctttttaact tagaaaaatc atccacaata tcatctcctt
    12841 aaagttcttt taaatatatt tttacaaaaa gcatctaatt gtatgcaaat tgacaatgta
    12901 aaataaagga aatgaacata aaatgtagaa taatttttat atgtatgtaa aaataaaaag
    12961 aaaaagttga aatttttaga aaataagagt attttttatc taaaaagaga gtttaaaaaa
    13021 taaaaaaatg ttgggaggat aaaaaatgag tttattagaa agtatatcaa aaaacatata
    13081 ttccctagat aactcttcta ttgaaaaaac aaagcagaga ttggataggc ttatacatcc
    13141 aactggaagc ttaggaaaga tagaagatat ttgcatgcaa ttagcaggaa tatttggcaa
    13201 tgaaaatttt gacacaagta aaaaggttat aattgctttt gcgggagacc atggtgtata
    13261 tgaagaaggc gtagcacctg accctcaaaa tataacaaaa ttacagtttc caaatttttc
    13321 gaaaggtcta tgcggagttg gagtaataag taaatttgta ggggcagatg ttgtagctgt
    13381 agatgtagga ataaactgtg atgaaaaact ggatggggta ttagactaca agataagaaa
    13441 agggacatca aatatggcaa aaggtccagc tatgagtaaa caagaagcaa ttagatgttt
    13501 agagataggt attgaaatag cagagcagtg tatagaaaaa gattataagg ttattggtat
    13561 tggggagatg ggtatagcaa atactactcc aagtacagca ataatatctg taatttcagg
    13621 gtgtgacccg ttagaagtta ctggtatagg tgcaggtctt aagaaagaaa gactaaagca
    13681 taaggctgaa gtaattagaa aggctattga aattaataat ccaaatccaa ctgatggagt
    13741 agatatttta tctaaagtag gagggtttga aataggctca atggcaggtg ttatattagg
    13801 ttgtagtgct aataggattc cagtagtaat tgatgggttt atttcttatg cagcagcatt
    13861 aattgcatat aagataaatc caaaaactag agaatatatg atagcatctc atttatcagc
    13921 agaatcagga actaaaagag cattagacat attaaaatta gaccctctat taaatatgga
    13981 tatgagatta ggtgagggaa gtggagctgc tttagcattt gatataatag aagcttcaaa
    14041 ctatacttat aaaaatatgg caacttttga tgaaattgac atgggaagat aaatataaaa
    14101 atttaatatg taggagtgtt ttgatgagta aaattattct agtaacagga ggagctaggt
    14161 cagggaagag taactttgca gaaaaactat gttttgacag aaataataat acagcttata
    14221 tagcaacctc tattccattt gatgatgaaa tgaaagatag agtaaaaaaa catcaagaga
    14281 atagacctag aaactggaat acttatgaaa tatatgaaga tatatattcc ataattaagg
    14341 agatatataa agaacatgaa actgttatac tagattgtgt aacattatta gtaaataatc
    14401 taatgtttga acacaatcta aatatagaag aatctagtca agaagaaata aacaaattag
    14461 aacaatacat aaaagaacaa gttgtcaaac ttattgaaga aatagaaaaa acaaatctat
    14521 actttgtatt tgtaacgaat gagttaggta tgggaattgt tcctgggaat aaacttagta
    14581 gaatttatgc tgatattgta ggtcgtataa atcaatatat agcttcgaaa agtaatgaag
    14641 tttattttgt agttagtggc atagctatga agataaagga gtagtatgca attatgaaaa
    14701 gatttatttt aatactacag tttttgacaa ggatacctat aaaattaaat gtaggatttg
    14761 atgatgaatt ttataagtca atagtatatt ttccacttgt aggttttgtc ataggtatat
    14821 tatcctattt aataggatgg atttctatgc ttttatttga accatttata gcttctataa
    14881 taataactct agctggtgtc ttaattactg gaggacttca tatagatgga ttaggagata
    14941 catttgatgc aatatatagt tatagggata aagaaaagat gcttgaaata atgaaagatt
    15001 caagattagg tacaaattca cttctagcaa ttatgtttgt acttttatta aaggttggat
    15061 tcgtttacga tataataagt aataattcat tgtgggttat aatttttatg ccaatgattg
    15121 caaggttagg agtaatgtta ttgacatata aaactgtgac accaagagaa aaaggaatgg
    15181 gaaatttatt tataggaaaa ttaactacaa gtatgttaat aactgctata atatacacat
    15241 tattgatagt tgctcttatt actaaattta tatttttatt acctaatata gtgttgatta
    15301 aagtattagg ttcgattata gtggtttttg tatttataat attattcaaa aaacatattt
    15361 ataaaaaaat tgacggagta acaggagata tattaggctg tggaatagaa ctttcagagc
    15421 ttgtatattt aatttacata tatttgttaa tttttatgtt tttttaaagt aaaaagtggt
    15481 gataatatga taagattaat attaataaga catgcactga caaatgataa taaaaaagga
    15541 agattatcag gccatataaa tagttgtatt agtgaagagg gcaaactaca aataaataaa
    15601 attaccagat atctgagtaa tgaaaacata gataaggttt atacaacacc atctacacgt
    15661 actaaagata cagtagaaaa aatatcaaaa ttgaaattat tagaaataga agaaaaagaa
    15721 gctttaagag aaattagttt tggagacttt gaaggaagaa cttttgaaga aattaaaatc
    15781 aaatatccaa atgagtttga aaaaatgata aaagaaggta acaactatag atatccaaat
    15841 ggagaaagtt tgatagactc ttataaaaga gtggcaaaag aaattgataa tataatttta
    15901 gaaaataata gtaatttaga tacaaagaca atacttatat gttctcatgc aggaacaata
    15961 agaaatataa taacacattt gatttctggt agttataagt atcactggaa ctttaaaatt
    16021 gataatgcat ctattacagt tttagaaatt gatggtggat ttgctgtaat agataaaatg
    16081 aattttacag acttcatata atttaatcaa aaccaaacat tacaatctgt gtttgtaata
    16141 tatctgtaaa attagcaatg aaaatagaat attagattaa aattaggttc ttaattagat
    16201 gttaaaaggg aaaaaggtta gatgcctttg cagcccccgc tactgtgaaa ccaacgaaac
    16261 tataaatacc actgtcagtt tgatgggaag gttatagaag taggatgaag ttaagccagg
    16321 atacctgcct aatttaattt aaaacaagag gtcttcgggg tgaaagattt ttaattaata
    16381 tatataaaag tcctatcttt agaagtatta ggactttttt taatttgttt tttagtaaat
    16441 tttaacaagg agggatggaa agtggggaaa aaaataatgc tacaaggaac agcttcaaat
    16501 gtaggtaaaa gtgtcttagt agcaggtttg tgtagaatat ttaagcaaga tggttatact
    16561 gttgctcctt ttaagtcaca aaatatggct ttaaattctt ttataactaa agaaggatta
    16621 gaaatgggaa gagcacaagt atttcaagca gaggctgcga aaatagagcc aatagctgat
    16681 atgaatccag ttttattaaa accttctgga aataataaaa gtcaagttat tgtaagaggt
    16741 aaagttgtag gagaaatgcc ttcttctaag tatcatgatt ataaattgga gttagtagat
    16801 gtgttgaagg aaacatttaa tagcttaaat tcaaggtatg atattgtagt tatggaaggg
    16861 gcaggaagta atgcagagat aaacttaaaa gatagagata tctctaatat gggtatggca
    16921 gaaatagctg atgcacctgt aataatagtc ggagatattg atagaggagg cgtatttgcc
    16981 tcattagcag gaacaatgct tttattaaat gaagatgaga aaagaagagt aaaaggtgta
    17041 ataatcaata aattcagagg aaaaaaagag cttttacaag atggtgtaaa gatgctagaa
    17101 gacatcataa aagtacctgt tataggagta gtaccttata cagatataaa aatagaagat
    17161 gaagacagtg taactacgag atttaagaaa aaaatgaata aaagtgatat acacatagaa
    17221 ataattagaa ccccacatat gtcaaatttt acagatttta atatatttga gacacaagaa
    17281 gatgtaagta taagatatgt agactatgga gaatctttag gaaatccaga tatattaata
    17341 atacctggaa ctaaaagtac tatagacgat ttaacatata taagaaaaag tggtcttgaa
    17401 tcgcaaatca aaaatttaca tagtcaaggt aaattgatat ttgggatatg tggtggatac
    17461 caaatgttag gtaaaaaatt aaaagaccct tatcatgttg aaagtgaaat tgaagaagtt
    17521 gatggaatag gtcttttaga tacagaaaca acgtttgaga aagaaaagac tacaacacaa
    17581 gtagaagcta ctattgttga atgtatcagt gaatacatga ataaccttaa gggaaaaaaa
    17641 gtaaaaggtt atgaaattca tatgggaatt acaaagcgag gtaatgatgt aagaagttta
    17701 gatttaataa ctaaaaaact tgataaagaa gtgaattata ctgagggaag tgtaaataaa
    17761 gaggggaatg taattgggac ttatcttcat ggaatatttg atgaaataag ctttacaaga
    17821 gcaattttaa acaatataag aaagaaaaaa ggtttaggag aattagaaag caaagtaaac
    17881 tcttttaatg aatttaagga taaagagtat gataagttgg ctgatttttt aagagaacat
    17941 ttagatatag agaaaatata tgagattatg aaataatgat gacctttatt tataatttag
    18001 gtaggaggtg tattatgaaa aaaattttaa ttgcgggtac taatagtgga gtaggtaaaa
    18061 caacaatatc attaggaata atgcaagctt tggttaagag aaatatgaaa gtacagccat
    18121 ataaagtagg gccagactat atagaccctt catatcacac ttttataact ggaagacatt
    18181 ctagaaattt agattcttat atgttagatg atgaaaaaat aaagtatata gtaaataagt
    18241 cctcaaaaga tgcagatatt tcagtaatag aaggtgttat gggcttatat gatggctttg
    18301 gaatagattt agataattgt acaagttcac atacttcaaa ggtattaaag tcacctgtta
    18361 tattagttat aaatggtaaa tctatggcag catctagtgc tgctatggta cttggatata
    18421 aggaactaga taaagatgta aatataaagg gtgttatagt aaataatgta aagactaata
    18481 gccattatca aataattaag agttctatag aaaaatattg taatgttgaa gtattggggt
    18541 attttcctcc aaataacaaa ttctctttgg aatctcgaca tctaggatta gttcctagtg
    18601 tagaaatgaa atcactaagg gaaaaatttt ataccttagc agatgaaatt gaacactata
    18661 tagatattga cagaataatt gaaatatcag aaagtgatga atttgagagt gattttgatt
    18721 ttcaaacttt gatagaaaat aataaaataa aagaacatgt taataataaa tcaatagcta
    18781 tagcatatga taaagcattc aatttttatt atagagaaaa tatagagctt ttagaggaac
    18841 ttggtttaga aataaactat tttagccctc ttgaagatgt atcagtgcca aatgcagatt
    18901 atatttatat tggaggaggt tttccagaaa tatttggaaa agaattagaa gcaaatgagt
    18961 caatgagaac ttccatatta gaagcacata acaataatat acctatttat gcagaatgtg
    19021 gaggactgat gtacctaggt gagaaactat tgaatcaaga agaacaagaa tttaatatgg
    19081 ttggaatatt tagtggaatt agtaagatga catcttcttt aaagagattt gggtactgta
    19141 ttggtgtagc caaggaaaat actcttttat ctaaaaaagg ggaatccatt aaagggcatg
    19201 agtttcatca ttcaatattt gaaagtgatg aaaaatgtgt atattcaatg agaaaagaaa
    19261 gagatggaaa tatagtagaa gagtgggatg gtggttatag taaaggaaac actttggcta
    19321 catatcttca tactcatttt tataataact taaattgtat agaaaatttt ttgaaaaaag
    19381 gaaatgaata aatgagtatt ttatctattt atataggata tgtgacagac ttaattgtag
    19441 gagacccata ttcttttcct catccagtga gatttattgg taaacttata aattttactc
    19501 aaggtataat aagaaaggtc tctaaaaatg ataggcagtt aaaatttggg ggatttatat
    19561 tatggtttat aaccgttgga ataacatatt taacaactta tgctatagtg aagttattta
    19621 gttttaatat attattgagt attgtagtga atagttttat aatttataca acactagcca
    19681 caaaatgttt aaaagacgaa gcattaaaaa tttataatgt attaaaaact gaagatatag
    19741 aaaaatctag gattcagcta tcttatatag taggtaggga tactactaat ttaagtgaac
    19801 ctgaaataat aagagcaact gtagaaactg ttgcagaaaa tactgtagat ggtataattg
    19861 cgcctatgtt ttatgcattt attggaggag caccacttgc tatggcctat aaagctatca
    19921 atacattgga ctcaacagtt ggatataaaa ataaaaagta taaagatatt ggttttgcat
    19981 ctgctaagat tgatgatata gccaattata taccagctag aatatctgtc attcttatga
    20041 caattggaag tttcttttta aattataact ataggaactg ttttaaaatc tctattagag
    20101 atagaaagaa tcataaaagt ccaaattgtg ctttttcaga aggtgcagta tctggtgctc
    20161 taggaataca actaggaggt accaacgttt attttgggga aaaagtatat aagccaacaa
    20221 taggggataa gcttagggag atagataaag aagatataat taaaactaat aaaattatgt
    20281 atgcatcttc atttgtatct atgttggtat ttacactaat atatgtgtta tgtaggatga
    20341 ttttaattaa cttaattata taaattattg tttgttaact taagggggac atagatgaag
    20401 gatttagggc atggtgccaa tgtagatgaa atggcaagat tatatgggaa aaacccaaaa
    20461 gaaataattg attttagttc aaacataaat ccaaatgtat taccaaattt agagagatac
    20521 attttaaaag gattagaaga gtgtaggaat tatccagata taaattatac aaacttgaga
    20581 gaaaatatat caaaatatat agatataaat ccagacttta taatacctgg caatggtgca
    20641 acagaagtga tttatttgct tatgaagagt attaaaaaaa aattggctat aataaatccg
    20701 actttttcag agtatagaag aagtgctgag ttaaataatt tagatattat agatttagag
    20761 ttagatttag aaaataactt taaacttaat atagatataa taaaagaaaa tataaaaaga
    20821 tttgatagtt tatttatatg taatcctaat aatccgagtg gtaatgtcca agatttaaag
    20881 gaacttgttc atttactaga taaacataat aaggtactaa taattgatga aactttcatg
    20941 gaatttgttg aagatgaaag taaatatagt ttggttaaat atatagaatc aaataaaaat
    21001 atatttataa ttaaagctgt gactaagttt tttggtatgc ctggtttaag gctaggatat
    21061 ggtcttacaa gcaacactga aattatgaac aaaatttatg aacacaaaga gccatggact
    21121 atcaattctt ttgcagatat actatccaat tttatttttg aagataaaga gtatattaaa
    21181 aatagtaaag aatattacat agaagaaaga aaatatatgt tacaagagtt gagaaacata
    21241 agaaatataa aggtctatga tacagatgct aattttatct tgataagaat atataaaaaa
    21301 acaactaaag aattgaaaaa agatttgttt aagcaaggaa acatattagt tagagatgca
    21361 tctaatttta taggattaga tgatagcttt ataagagtgg ctataaaatc tcatgaagat
    21421 aacaaaattc ttatagaaaa tatgaaaaat ttattgggtg attaaaatga aatcttatgg
    21481 aatatgccca gcatcttgtg gagaatttgt acaaggtata attgatgatg aagaatattt
    21541 atgttcatat gcaatagata tgtattctaa agtgtatatt gaagaaaaat tagtagatat
    21601 aaatttaggg agatataagt caaggttagc aattgaaaag gtatttgaaa aatttaatct
    21661 acctaaaaaa tatactaaaa atatttcttt aaatataaat agtaaaatac ctgttggaaa
    21721 aggaatggca agttcaactg ctgatatagg agctactata aaggcaacat tatcattgat
    21781 agataaagat ttaagtagcg aggagatttc taaattagca gcagaaatag aaccaacaga
    21841 ttctatattt atagataaaa atagtatatt taatcctcta aatggaactg ttataaaata
    21901 cctaggaaat ctgaccaatg cgaaagttgt aatacttgaa ccaaacaaag tactagatac
    21961 tatgaaaatt agattgaggc aagattataa taaattaaaa gtagagaata aagaagtaat
    22021 aaaaaaatca tttgctcttt tagaagaagg attgaaaaaa aataatttat ctctagttgg
    22081 agaggcttgt actttgagta gcttagcaaa tgagaatata gaaaaaaaag agtatttaaa
    22141 tgaaataata aaaatatcaa aaaaatatgg agcatatgga gtaaatattg ctcacagtgg
    22201 aactgtagta ggtattttaa ttgataagtt tatgaatgat aaaaagatga tagatgcctt
    22261 atgtgaatct aacataaatt ctgtctataa taaaatttat acacaaaaca taattaatgg
    22321 aggaattaag ggggaaatag aatggaatac ataaagaatc ctatgaaaat agaagaaaaa
    22381 agttttgaat tgatacaaga aataatagac gaaattagac cagattataa atttaagaat
    22441 aatatcgaag aaaaaataat aaaaagagct atacatacta ctgctgattt tgattactta
    22501 gatatattaa aaatatcaga agaagcagtt gatagtataa tagatgcatt aaaaaataat
    22561 gcaagtattt atactgatac aaatatggct cttagtggta taaataaaag aaagttagaa
    22621 tcattaggat gtaagtataa atgtttagtg gcagatgatg aaactgtaaa attagctaaa
    22681 gaaaaaggaa ttactcgttc tatggcagca gtagaaattg cagctaaaga agaaggaaga
    22741 aaaatattcg ttttaggtaa tgctccaaca gctttatata aagttatgga gatgaaatca
    22801 gaaggtagat tagatttaga tgcagttata ggagttcctg taggttttgt aggagcacaa
    22861 gaatcaaaag atgaagttga aaaaacagat ataccatata taatttctaa aggtagaaaa
    22921 ggtggcagta atttagcagc agcaatagta aatgctatat tatatactat gtaggtacct
    22981 atatggaaga atatgtatat atagatggaa aaaaatatag aagaggatat actacaggtt
    23041 catgtgcaac aggtgcatct aaggctgctg tatatatgtt gattacaaaa aatagaatca
    23101 atactataaa tatagatact cctaagggga taccattact actaaaagtt gataatataa
    23161 atatttctga tacatttgtg gaatgttcca taaaaaaaga tggtggagat gatatagatg
    23221 caactcatac catggatatt tatgcaagag ctgaaattgt agctaaaaat gataagaaca
    23281 aagggtatct tactctaaag gatatagata atttaagcac caatagtgag tgtaaaagtg
    23341 agctctataa gtttattagg gtttatggcg gaactggtat tggtgtggtt acaaaaaaag
    23401 gtcttagcgt ggatgttggt aaacctgcta taaatccgac tccacttaaa atgataaatc
    23461 atgaaataag aaagcttata ggagataatt ttgaatctat acttggaaat gataaagttt
    23521 taaaaataac aatctttgct ccacaaggag aaactgtagc aaaaaaaaca tttaatccaa
    23581 gattaggtat agttggagga atatctataa taggtacaac tgggattgtt gaacctatga
    23641 gtgatgaggg ttggaagaag tctctatcta tagaacttca gatgaaaaaa gaacaaggct
    23701 tagataaaat aatattggtt ccaggaaatc atggagaaca gtttataaga gaaaaattaa
    23761 acttagatat taagtatgta gttagggtaa gtaattttat aggctatatg attaaagaag
    23821 ctcaaagaat aggatataag aaaatcttaa tggcaggtca tataggtaaa tttataaaag
    23881 tatctgctgg tatttttaat actcatagta aggtagcaga tgctagaagt gagatattag
    23941 tggcaaactt agctttaatg ggagctagat acgaattttt aaataaaata aatcaatgcg
    24001 taactactga agaagctgta gagctaataa ataatagtga atatagagaa gtatataata
    24061 tattaagtaa taaatgtagg gagagagtaa agcaatactt gaatgaagat tcagatgata
    24121 ttgatgtgga agtaatcata ttttcaatgg ataagtcttt attaggtaaa tcggataata
    24181 cagatgattt agtggaggtc tttatatgat aaacataata ggtctaggac ctggtaactt
    24241 agactatata acaaaaaaag gtgaaaattt aatttccact tctgatgtat taattggtgg
    24301 taaaagaaat ttagagtcta taaaaaattt tgaaggagaa aaaatagttc tggattctaa
    24361 tttaagagaa attatagagt acataaataa caataaagaa aagcaaatat caataatagc
    24421 atctggggac ccactaatat atgggatagg tagatatcta tctaaaaata ttgataataa
    24481 gatgttaaac atggtttctg ggattagttc attacagtac atattttcaa aaatatatgt
    24541 agatatgaat gatgtatata ttacaagtag tcatggtaaa gtaccagatt ttgattacat
    24601 attatcacat aaaaaagtat gtatggttac agattcaaag ataggaccaa aacaaataag
    24661 tagagaaata atagatagaa atctaaataa aattattgtt gtaggagaaa atctgtcata
    24721 tgacaatgaa aaaataacaa tagcaaaacc agaagagata ataagaatgg acaattttga
    24781 tatgaatgtt gtggtgattt tagatgagga atagtgaatt tataacaggt aaagttccaa
    24841 ttactaagga agaggttaga gcaatatcaa taagtaagtt aaatttagta aatgctaaaa
    24901 acctcataga cataggggct ggtacaggaa gtgtaagtgt tgaagctgca tataattatc
    24961 ctaatttaaa ggttatatct atagagaaaa atgatgatgc tatagagctt atagagaaaa
    25021 ataaaaaaaa attcaacttg aaaaatatag aagtgataaa aggatatgct ccaattaatc
    25081 tagggttaaa tgttaaagtt gagtctattt ttcttggggg aacaggaaat aatctggaag
    25141 aaataataga atggtctaaa aaaaatctcg ttacaggagg aagacttgta gctaatttta
    25201 taataataga tacatttaat caaactttaa aactgcttaa aaagcatgga tttaaagaaa
    25261 tagatgtatg tgttttgaat gtttctaaac tagagaaact aggaaaaggt gaatatttta
    25321 aaccactaaa tcctatatat ataatatctt gtgaaaaagg ggaaaatgat gatgaataaa
    25381 gtacattttg taggagcagg acctggagat aaagagctga taacattaaa aggatacaaa
    25441 ttattaagta atgcagatgt agtgatatat gcaggttcac ttgttaatcc tgaacttttg
    25501 gaatactgta aagaagattg ccaaatacat aatagtgcac atatggattt acaagagata
    25561 atagatgtta tgagagaagg tatagaaaat aataaatctg ttgtaagact gcaaacaggt
    25621 gatttttcta tatatggttc aattagagaa caagtggaag atttaaataa attgaatata
    25681 gattatgact gtactcctgg agtaagttct tttttagggg cagcatcatc tttaggggtt
    25741 gaatatacag ttccagaaat ttctcaaagt gtaattataa caagaatgga aggcagaact
    25801 ccggttccag agaaagaatc aatacaatca tatgctaagc atcagacatc tatggtaata
    25861 tttttatctg ttcaagaaat tgagaaagtt gtgtctaaat tactggaagg tggatatcca
    25921 aaagatactc ctatagcagt tatatacaaa gcaacttggg cagatgaaaa aatagttaaa
    25981 ggaactttaa gtgatatagc tgtaaaagtt aaagaaaaca acataaataa gactgcttta
    26041 ataatggttg ggagattttt aggagaagag tataataatt caaagttata tgataaggat
    26101 ttcaaacatg aatatagagg gtaatattgc tataatctgt attacagaaa atggaaaaaa
    26161 attagctcta aagataaatt ctctagtaaa taactgtgtt atatatcaag taaaaaataa
    26221 aaaaagtaat ttagttgttg aagaaacatg tgtgcataca gttcaaaaga agttaagtga
    26281 ttttgtagga gaaatattta gcaaatttga ttacatagta tttataatgg ctacaggtat
    26341 agttgtacga agtatagcac catacataat aagtaagttt agtgaccctg caattttggt
    26401 aatggatgaa aaaggcaaaa acgtaataag tttattgagt ggtcatattg gaggggcaaa
    26461 cagcttgaca cagtacataa gtagtcttat tgatgctaat ccagtaataa caacagctac
    26521 tgatataaat caaaaagctt ccttagatat gatagcaaaa aaactaaatg cacatattga
    26581 taattttaga gaaagtgtaa aagaagtaaa ttctatgcta gtgaataata aatgtgttgg
    26641 attatatgtt gatgaaggat atcaagttga tactagggga tttaaactta tagaaagtaa
    26701 ggatgagtta agagatagtt tttcaaacac atatatagaa aaattagttg tagtaagcaa
    26761 taaaataaat tgtattgaag atattgagaa tttaaaccta gataataaat ttgaacattg
    26821 ctatagtacc caaaaaataa taaaggtaat tccaagagat atagtgatag gaattggctg
    26881 tagaagaaac acagatagtg aactgatgta taattcactt gaagagtttc ttaataaaca
    26941 aaatatagat attagagcaa taaacacagt tggaagtata gaactgaaaa aagacgaaaa
    27001 agcaataatt gaattatcaa aaatattgaa ggtgccattt aaaacatttt cagtagatga
    27061 aatatctaag gttgataatc tttttgataa atctgatttt gttaaaaaaa atgtgggtgt
    27121 gtattcagta gcagaacctg tagcatatct tttgagcaaa ggtaatttgt tagttgaaaa
    27181 acataaatat aaaggaataa ctttttcagt ggggagaata aaaatatgat atacgttgta
    27241 ggaattggac caggaagtaa agatacaatg actttagaag caataaaagc aatagaagat
    27301 tctgaggcta ttgttggata taagacttat ataaaactaa tagaagaatt tatacaaggt
    27361 aaagaaataa tccaaaatgg tatgaggcaa gaagttgata gatgtaaaca agcagtggag
    27421 gaagcaaaaa aaggaaaaaa agtagcagtt gtaagcagtg gtgatgctgg aatatatggg
    27481 atggcaggtc ttattttaga attaatatct aaagaagatg aagatataaa agtaaaggta
    27541 attccaggcg ttactgcaag tattggtgca gcagctatac ttggagctcc tattatgcat
    27601 gatttctgtc atataagttt gagtgattta atgactccgt gggaagtaat tgaaaaaaga
    27661 ttgagattgg ctgctgaagc cgattttgta atttgtttgt ataatccaag aagtaaggga
    27721 agaagtgaac atctaagcaa agcattcaaa ataatgggtg agtttaaagg tggagataca
    27781 cctgttggag tggtaaaaga tgtaggtaga gaaaaagaag aaaagtttgt atgtactttt
    27841 gaaaatatgg actttgaaaa agtcgatatg actacaatgg ttataatagg aaataagtct
    27901 acctatatta atggtgaatt gatgataact ccaagaggtt atacagtatg attttaattt
    27961 taggaggaac ttcagatagt ttatctatat gtgataagat aaatgaactg aaaaatcaac
    28021 catatatcct ttcagttact acagattatg gaagggaact tgcacaacaa catgcagaga
    28081 atgtaatttt aggtaagctt ggaaaagaag atatgctaaa atttataaaa gataataata
    28141 tagtaaaaat aatagatgcc actcatcctt atgcagttga agtatccaaa actgctatta
    28201 cttgtgcaaa actattgaat atagattata taaggtttga gagaaaatct ttaattgaag
    28261 aaatagatta tgataataaa tttatagtag atacaattga agaagcctgt gaaatagcaa
    28321 ataaaatagg gacaaatata ttcataggaa caggaagtaa aaatttgaac agatttatag
    28381 aggaaataag tgataaaaat ttagttgtta gagttcttcc aacgagtgaa gtcatattaa
    28441 gttgtgaaaa attaggttta agtgcagata atatagtagc tatgaaaggt ccatttagcc
    28501 agtatataaa tgaagaaaca tataaacatt acaatataga tttagtcata acaaaagaaa
    28561 gtggaatagc tggtgggttc ttagagaagg taaatgcatg taaaactttc aatataccag
    28621 taataattat aaaaagagaa tcgattaact acgaaaatgt atttaatgac ataaataaaa
    28681 ttaaagacat acttgtgtaa tttttaggag gagaatatat gaagaaagcc ttattagtag
    28741 tgagttttgg tacaagctat catgaaacga gagaaaagac aatagatata tgtgaagata
    28801 aaataaaaaa ttcgttaaaa agccatgatt tttttagagc ttatacatca aatatgataa
    28861 taaataaaat aaaaaaaagg gatggaatag aaatagataa tccaatacag gctttagata
    28921 aaatatatga acaaggatat gatgaagtaa taatccaaac acttcatatt atatgtggcg
    28981 aagaatttaa caaattaaaa gaacaagtag atggatattc ttctaaattc aaaaagattg
    29041 tattaggaag acctttgctt acacatattg aagattatca ggaagctgta gaggcaatta
    29101 agcatcaaat tcctcatatg gaaagtaatg aagcagtagt atttatggga catggaacac
    29161 ttcatgaatc acattcagcg tatccagcat tggaatacat gcttagggat aatggaataa
    29221 atgcttatgt tggtacagtt gaagggtatc cagaaataga acatgtaata agaagattaa
    29281 aagaaggaaa tataaaaact gtaaatttga tgccatttat gttagttgca ggagaccatg
    29341 ctataaatga tatggcagga gatgaagaag attcttggaa aacaatatta gaagaaaatg
    29401 gatttaacgt aagagttcat ttaaaaggac taggagaaaa ttcttatata caagataaat
    29461 ttgttcgtca tgctgtaaaa tgtgcagaga atgcgaagtt ctatggtata ggagcagggc
    29521 caggagatgg ttctttactt acgataaaag cggttaatac tttaaagaaa ctggatattc
    29581 tatacactcc agaatcaaaa aagggtggag atagtctagc tttatcaata gttagtgaat
    29641 atttacctga atcagtggaa ataaagtcta ggcatttccc aatgagtttt gatgggaatg
    29701 aaaaaagttt agcttgggac aatgtggcac aagaaattat atcagatgtt aatgatggta
    29761 aaaatgtagg atttgttaca ttaggagacc ctatgattta tagtacatat gtatatataa
    29821 tgaaaagaat attaggagag gtagaagtcg aaactatacc aggaatttca tcattttcca
    29881 atatagcatc taatcagaat tttccattag tcatggataa agaagcttta gtggtaattc
    29941 cttgtactat ggaagatgat aagatagagt atgctttgca aaattataat tcaatagtat
    30001 tgatgaaagt atataaaaat tttaaagaaa taataaaaaa attagaaaag tatgatttaa
    30061 tagaacatgc tattttagta agtaattctt cccaagaaag cgaaaatgtg tttacagatt
    30121 taaaagaagc gcaccttgaa gataagatat cttatttttc tactatacta gtaaataaag
    30181 ataataaaat aaaataggtg aaagcaatat ttaaaggaga cttaatatga atatagtggt
    30241 tggaactagg ggtagtaatc tagcgcttat acaaacagaa tgggtaataa atgaattaaa
    30301 aaagaagtat ccagaaatta gttttgagat taaaatcata aagacaaaag gagatttaat
    30361 tcaaaatgtt tcactggata aaataggtga taaaggattg tttgtcaaag aaatagagca
    30421 acaattatta gatggaaaaa tagacatagc agtacatagt atgaaagata tgccttctta
    30481 tttagcagat ggactaaaat ttgctcatac gcctaagaga gaagacccaa gagatgttct
    30541 aatacttaga gaaggatata aaaatttaga tgatttgcct catggagcag taataggaac
    30601 aggaagtaaa agaaggaagt ttcaattatt gaaacaaaga cctgatttaa atatagtaca
    30661 agttagaggt aatgtggaaa ctaggatacg aaaaataaaa gatgaaaaca tgcatggtat
    30721 tgtgttggca gcatcaggaa taattagagc taatttacaa gataaaataa gttcttattt
    30781 acctgttgat gttgttattc cagcacctgc ccaaggagct ttagctatag aaattagaag
    30841 caatgatagt acgatagagg gaatagtaaa ttcactgaag gatgagaata cagaaatcca
    30901 aatattggct gaaagaggtt ttttagatgg tgtaaatgga agttgtcata ttccaatggc
    30961 agcttattgt gaaataatcc aagataaaat acatcttact ggcttatatg gagattcaga
    31021 aggaaaaaaa gtagtaatca agtctataga tggagatatt tcttctccta gagagttagg
    31081 tttaaagtta gctaaacttg tattgaagga gtatgagaat tatgaagggt aaggtttact
    31141 tagtaggggc aggaccaggt gattataaac tcatgacttt aaaaggttta gagtgtataa
    31201 gaaaatcgga tgtaatagta tatgatagat tagcaaatag tagttattta agagaagcta
    31261 agcctgattg tgaacttata tatgtaggca aggcatcaag taatcatatt ttgccacaag
    31321 aagatataaa tagaattata gcagaaaaag ctaaagaagg aaaaattgta actcgtctta
    31381 aaggaggaga cccttatgta tttggcagag ggggagaaga agcagagact ttagtagatg
    31441 aaggtataga atttgaagta gttccaggta taacttctgc tataggaggt ttatgttatg
    31501 cgggtattcc tataacacat agagaccatg cttcgtcatt tcatgtaata acaggtcatt
    31561 tgaagggtga tgatagtgga gaattaaact ggaatgctct tgcaaataac aaaggtactc
    31621 ttgtgttttt gatgggaatt tcaaacctag agaaaataag tgaaaactta atgaaagaag
    31681 gaaaagataa ggatacacct gttgctctta taagttgggc aactagatat aatcaaagag
    31741 ttgttacatc tacacttgaa aatgtttatg aaactgctat taaagaagaa gtaaaaccgc
    31801 caactcttat agtagttggg agtgttgtag gacttagaga aaagttgaac ttctttgaaa
    31861 gtaagccttt atttggaaaa agtattgctg taactaggtc tagaaatcaa aatagtgtat
    31921 tggtagaaaa aataatggat ttaggtggaa atcctataga aatacctact attaaggtag
    31981 aaaaaataca aaataatatt aacttagaaa atgaaataaa aaatataaat aagtataatt
    32041 atctaatact aactagtaaa aatgccgtag aaatcttctt tgaaaagata tatgaaatga
    32101 attttgattt aaggatatta tctaatttaa aggtatgtgc tataggttct gctacatcaa
    32161 atgagttgaa aaaaagagga attatagcgg atatagtacc aaaaaaattt gtagcagagt
    32221 cactgtatga agagttagcg cctattttaa atgatgacga taatatactt atacctagag
    32281 cagaaaatgc tagagattat ctggttgaca aactcaaggc tatttgtgaa gtaacagaag
    32341 tacatatata tagaacagta atagatgagg agaaaaaagg tgagatatta gatattttaa
    32401 attctaatga catagattat ataacattta ctagttcctc aacagtaaat aatcttgttg
    32461 aaattatagg aaaagaaaat attaaaatat tagaaaaaat aaaaacacta tctataggtc
    32521 ctataactac agagacaata aatagtttgg gaataaaatt gtacaaagaa tctgctacat
    32581 ctactataga ttcgttagta gatttaatta taaaaaatta attttggagg cttgaatatg
    32641 ttaagaagac caagaagact aagagcaagt aaagcaatca gaaatttggt aagagaaact
    32701 aaggtgaatg tagaagactt aatatatcca ctgtttgtag tagaggggga aaatataaaa
    32761 gaagaaatta aatctttacc agatgtctat catttatcaa tagatatgct agaagacgaa
    32821 ataaaagaaa taagtgagtt gggaattgaa catataatgt tatttggtgt tccagatgaa
    32881 aaagatgagt gtgcttcaca agcttttaat gataatggaa tagttcaaaa ggcaattaga
    32941 aaggtaaaag aaataaattc agatataaat gtaataacag atgtctgtat gtgccaatat
    33001 acaagtcatg gacattgtgg gattttaact gatactggtt atgttgataa tgataaatca
    33061 ttggagtatt tggctaaaat atctgtaagc catgctaaag caggagctga tatggttgct
    33121 ccatctgata tgatggatgg aagaatacaa gcaataagag aagctttaga tgaaaatgga
    33181 ttagaaaatg ttggaatcat ggcttatagt gttaagtatg cttcaaatta ttatggacct
    33241 tttagagagg cagctcattc agcaccatct tttggagata gaaaaacata tcaaatggac
    33301 ccagcaaata gcaatgaagc tataatagag actgaacttg atatattgga aggtgcagat
    33361 atacttatgg ttaaacctgc attatcatat ttagatatta taagaagagt taaagataat
    33421 tttgacttac ctctagcagc ttataatgta agtggagaat actctatgct taagtttgct
    33481 gtgaaggaag gtttattaaa tgaagatgta attcttgaat caattacatc tataaaaaga
    33541 gcaggagcag atataataat aacttatttt gctaaagaca ttgctaaact gttgaaaaaa
    33601 taaattctag cagtataaag aatatcaaaa agtataggaa ttgtaattaa ctattataca
    33661 gtagttttgt ttagacattt attgaagaat aatagaaact aatttgcaat ttctatactt
    33721 tatttatgta actaaataaa atttggaggt agaagatgct atatcctatt aatttaaaat
    33781 tagatgagtt agatgtagtt ataattggtg gtggagaagt tgcatatagg aaatgtaaaa
    33841 attttttaga gtttaacaaa aatgttacta tagtttctaa acaaatttta aataaatttt
    33901 atgatttaaa aggaaatata aaaataataa aagatgatta taaagaaata tatataaaag
    33961 atgcttctgt aataattgct gccacaaata atagagagtt aaatatggaa ataggattat
    34021 attgtaaagc aaaaaataag ctagtgaatg tagttgataa tatagaaata tcgaacttta
    34081 ctgttccatc atatataaaa agaggagatt tacttataag tgtgtctact gggggcaaga
    34141 gtccttcatt atcatcaaag ataaaaaaag aaattgaaga gagatatact gaagattatg
    34201 aggagtattt aaatgtatta ggagatataa gaaaaaaggt tataaaaaaa tacgaagata
    34261 aaagtaaaag aaaagatgtg cttaatatgt tggtaacact tgacctagaa gaacttaaga
    34321 agtttgatat ataaaaaata aaataaaaaa tgttaaaaag gtgttgacgg attattttta
    34381 ag
//
LOCUS       DAYAOX010000016        33555 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.016, whole genome shotgun sequence.
ACCESSION   DAYAOX010000016 DAYAOX010000000
VERSION     DAYAOX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33555
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.016"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            154..996
                     /locus_tag="ACGI57_000656"
     CDS             154..996
                     /locus_tag="ACGI57_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HFG9715743.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            1011..1475
                     /locus_tag="ACGI57_000657"
     CDS             1011..1475
                     /locus_tag="ACGI57_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715744.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1489..2634
                     /locus_tag="ACGI57_000658"
     CDS             1489..2634
                     /locus_tag="ACGI57_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9715745.1"
                     /translation="MNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPELAFEEYKSQ
                     KALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPGMGHACGHNI
                     IGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFNNVDAALILH
                     PSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSVNSMRLHLKD
                     YARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKGAAISTGTNV
                     EIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGNLTHEIPAIQ
                     AYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            2678..3613
                     /locus_tag="ACGI57_000659"
     CDS             2678..3613
                     /locus_tag="ACGI57_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HFG9715746.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(3887..6580)
                     /locus_tag="ACGI57_000660"
     CDS             complement(3887..6580)
                     /locus_tag="ACGI57_000660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFG9715747.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            6794..7492
                     /gene="yunB"
                     /locus_tag="ACGI57_000661"
     CDS             6794..7492
                     /gene="yunB"
                     /locus_tag="ACGI57_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HFG9715748.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(7704..8711)
                     /locus_tag="ACGI57_000662"
     CDS             complement(7704..8711)
                     /locus_tag="ACGI57_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HFG9715749.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            8939..9823
                     /locus_tag="ACGI57_000663"
     CDS             8939..9823
                     /locus_tag="ACGI57_000663"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9715750.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            10025..11575
                     /locus_tag="ACGI57_000664"
     CDS             10025..11575
                     /locus_tag="ACGI57_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9715751.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            11604..12251
                     /locus_tag="ACGI57_000665"
     CDS             11604..12251
                     /locus_tag="ACGI57_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFG9715752.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(12393..12653)
                     /locus_tag="ACGI57_000666"
     CDS             complement(12393..12653)
                     /locus_tag="ACGI57_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HFG9715753.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFRYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            12870..14153
                     /gene="hflX"
                     /locus_tag="ACGI57_000667"
     CDS             12870..14153
                     /gene="hflX"
                     /locus_tag="ACGI57_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFG9715754.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYANLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDADLDEEDFNIYREYILEK"
     gene            14161..14838
                     /locus_tag="ACGI57_000668"
     CDS             14161..14838
                     /locus_tag="ACGI57_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFG9715755.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            14924..15367
                     /locus_tag="ACGI57_000669"
     CDS             14924..15367
                     /locus_tag="ACGI57_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFG9715756.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            15672..16316
                     /locus_tag="ACGI57_000670"
     CDS             15672..16316
                     /locus_tag="ACGI57_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFG9715757.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(16402..16779)
                     /locus_tag="ACGI57_000671"
     CDS             complement(16402..16779)
                     /locus_tag="ACGI57_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFG9715758.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(16779..17165)
                     /locus_tag="ACGI57_000672"
     CDS             complement(16779..17165)
                     /locus_tag="ACGI57_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFG9715759.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            17344..18099
                     /gene="nadE"
                     /locus_tag="ACGI57_000673"
     CDS             17344..18099
                     /gene="nadE"
                     /locus_tag="ACGI57_000673"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFG9715760.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            18182..18901
                     /locus_tag="ACGI57_000674"
     CDS             18182..18901
                     /locus_tag="ACGI57_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9715761.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(19068..20072)
                     /locus_tag="ACGI57_000675"
     CDS             complement(19068..20072)
                     /locus_tag="ACGI57_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFG9715762.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            20338..21234
                     /locus_tag="ACGI57_000676"
     CDS             20338..21234
                     /locus_tag="ACGI57_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HFG9715763.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            21355..22617
                     /locus_tag="ACGI57_000677"
     CDS             21355..22617
                     /locus_tag="ACGI57_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFG9715764.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            22693..23886
                     /locus_tag="ACGI57_000678"
     CDS             22693..23886
                     /locus_tag="ACGI57_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HFG9715765.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            24515..25309
                     /locus_tag="ACGI57_000679"
     CDS             24515..25309
                     /locus_tag="ACGI57_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HFG9715766.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDVAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            25404..26744
                     /locus_tag="ACGI57_000680"
     CDS             25404..26744
                     /locus_tag="ACGI57_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFG9715767.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            26881..28113
                     /locus_tag="ACGI57_000681"
     CDS             26881..28113
                     /locus_tag="ACGI57_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HFG9715768.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     TLIESGIVTMNKEACEIC"
     gene            28153..29295
                     /gene="acrC"
                     /locus_tag="ACGI57_000682"
     CDS             28153..29295
                     /gene="acrC"
                     /locus_tag="ACGI57_000682"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0043958 - acryloyl-CoA reductase (NADH)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HFG9715769.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            29306..30100
                     /locus_tag="ACGI57_000683"
     CDS             29306..30100
                     /locus_tag="ACGI57_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HFG9715770.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            30114..31133
                     /locus_tag="ACGI57_000684"
     CDS             30114..31133
                     /locus_tag="ACGI57_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFG9715771.1"
                     /translation="MRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLAD
                     QLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERKK
                     PEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMATI
                     VCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSVN
                     LTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGTT
                     IRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEMI
                     ESLNR"
     gene            31232..32608
                     /locus_tag="ACGI57_000685"
     CDS             31232..32608
                     /locus_tag="ACGI57_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFG9715772.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(32940..33284)
                     /locus_tag="ACGI57_000686"
     CDS             complement(32940..33284)
                     /locus_tag="ACGI57_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715773.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT        12445 a         4109 c         6149 g        10852 t
ORIGIN      
        1 tcatgaatct ataatatttt tttgattcat aactaaaagt atataaaaat gatgaataaa
       61 tatatattat acattatcaa tgataggtta tataataaaa tcactaacaa ttatattata
      121 agaaaattat aaaaattaca aggaggtata gaaatgaaag aaaaagcagg gattagaaat
      181 gccataatag cagcctttat aatcacaata ttggcagaat taattggacc tatttcattt
      241 aaagttatgg gaattaatgt aactttactt agtatactat gggctatatt cataggaatg
      301 gctgtttctc ctcatttatt agggagagtt ataccagcct taaaaaagtt tataggggat
      361 aatgaaataa atgtatctcc atatttatta tctttgacac tatatccatt agggataatg
      421 tttggaatta atgcagggcc taaagttggt atattacttc aagcaggacc agcactttta
      481 ttacaagaat ttggtcatat gggtacaatg ttgatagcat tacctgttgg agtagcatta
      541 ggtcttggaa gaagtgcatt aggtggtact ttttcattat gtcgtgacac agcactagga
      601 attattggag ataagtatgg attaaactca ccagaaggta taggaacact aggaacttac
      661 ataagtggta gtatatttgg aacattatta ttttcatttt tagcaccagt gggtgtactt
      721 ttaggatttc atccatatgc tttagcaatg gcatcaggaa tgggtagtgg ttcaatgatg
      781 ggagctgcaa cagcatcatt aatgaataca gttccacaaa tgtcagagca gattttagca
      841 tattcagcta ctagtggatt gattacagct gttactggaa tatatataga attattctta
      901 tctttaccag tagccaacta ctattatagc aaagtagctc cagttattga aagatttaga
      961 ggaagaaaaa caagaagaac aagtaataaa ttgtaaagga ggatatatat atggaaaaaa
     1021 cttttaagac tattatggag cagataaaaa tagtattgtt tatatatatt attatttgct
     1081 ttggacaaat gatagcactt aagactcagg ctcaagattt gttgattgga agtgtatctg
     1141 gatttttgtt ggtgataatt gctattataa taaagaattt tgttaaaaaa cctaatttac
     1201 caggttttgc atgggctaca ttagttgcat ttgttttatc gcttccaata tctccagtgg
     1261 gggatatcat agcaacaaat gtagggaaaa tcaattttat gtctacagta acaccattat
     1321 tagcttttgc aggaatctct gtaggtaatc aattagatat attaaagact ttgagttgga
     1381 agttagtagt tatatcattt atagttatga catctactta ctttggttct gcttttatat
     1441 ctcaaacagt tttaagatta aatggaatgc tttaaggagg tagtaaatat gaatgaatta
     1501 aaagcaaaaa ttaaagagga aataaagtta ttatctgaag agaaaaagac atcatttgaa
     1561 aaagtttctg actatttatt ttcaaatcca gaattagcat ttgaggagta taagtcacaa
     1621 aaagcattat gtgatttgtt agaagaaaat gaatttaatg taactaaagg tgtaggagga
     1681 ttagaaacat cttttgaagc tgtgtattca aatggaacta atgggaaaac agttgctttt
     1741 ttagctgaat atgatgcact tccaggaatg ggacatgcat gtgggcacaa tatcataggg
     1801 acaagttctg ttggtgcagg tatcatatta aaagaaatca tgaaaaaaca taatatagaa
     1861 gggactgtaa aggtttttgg aacacctgca gaagaaagag taggtggaaa aataactatg
     1921 attaaagaag gtgtatttaa taatgtagat gctgcattaa ttttacatcc aagtgatgct
     1981 tcaatgcctg atgatatatc atttgctcaa gttaatttaa aatttgattt tactggtaag
     2041 gcatcacatg ctgctgcttt tccttgggaa gggaaaagtg ccttaagtgg agtaatagca
     2101 ctatttaaca gtgtaaatag catgagatta catcttaaag attatgcaag agtacatgga
     2161 attataactg atggaggtag catacataat ataattcctg aaaaatctac agctatattt
     2221 aatgtaagag ccttaagtat agaatattta aatgagattt gtgaaatgct taaaaattgt
     2281 gctaaaggag ctgcaatttc tactggaact aatgtagaaa tagttcaatt agatgaaata
     2341 tacaaagaga ttaagaatga ttctgaatta gtgaatatag ttagacaaaa ttttgaagtt
     2401 ttgggtgaag actatgttga aagagattta agtcaaggta taggttcaac tgatacagga
     2461 aatttaactc atgagatacc tgcaatacaa gcatatataa aattaaaaga aaatactgca
     2521 actcatacag atgaatttgc tgttgcagct ggtggtgaag aaggcagagt agcacttata
     2581 aaagcaatta aggtgttagc aatgtgtgga gtagatattt tgtatagtaa ataatatata
     2641 attttaaaat atattaagaa aggaaatcgg attagacatg atattaaaac aagtaataga
     2701 ggtttatgac attttagata aagcaaatgc taatggggaa gaagtaaaaa cttacttaca
     2761 aggttatggt gaagtagatg taactgtaaa agaattgtct tcaagtaaag gttcaacaga
     2821 tttagtaaag cttacaattc ctggaaaaaa tggaaaatta aaaggtggag atgctcctac
     2881 tcttggtatt ttaggtagac taggagggat tggagcaaga ccagaagtta taggttttgt
     2941 ctcagatggt gatggagcat tggtagcaat tgcagtggct gctaaacttt tggatatgca
     3001 acgaaaaggg gatatattag atggtgatgt tgtaatttca actcatattt gcccagatgc
     3061 tccaactaaa gaacactatc ctactccttt tatggattct ccaattgata tgatgactat
     3121 gaatgaaaat gaagttgata gtagttgtga tgcaatactt tctatagata ctactaaagg
     3181 aaatagagta atcaatacta gaggatttgc aatatcgcca actgttaaag aaggctacat
     3241 attaaaaact agtgataacc ttttagatat tatgcagact gtaacaggga aatctccatt
     3301 tgtattccct ttgtctattc aagatataac accatatgga aataatctgt atcacttaaa
     3361 tagtatttta caaccagcag tagctacaga tgcaccaata gtaggagtag caattacaac
     3421 agagatgcct gttgctggat gtgctacagg tgcaactaat tttagtgatt tagattcagc
     3481 tgggagattt gcaattgaag ttgccaagct atttggtaga aataagtgtg atttctatga
     3541 taaagaagag tgggaaatgc ttattaagag atatggcaaa ttaaatagat ttcaaacatt
     3601 tggtatacag taattagaaa aatattaagt ttatataatt tgtgatataa ctatttaaga
     3661 atatataaac aattaggaat aaaagtaaga ataaagcact tagttaaaaa ctttaaaaat
     3721 aaaatattta tttgtttata taaaaaggag gattctggtt attgggattc tcttttttat
     3781 gaatacaaat aatatattgt aattataaaa aaaggagcgt ttcaaagtga tttttttaat
     3841 caccttgaga caactcctta tatttttatt taaattctat tttttattaa gttgctggag
     3901 ttccaccacc atcaggagga gtagtagagc caccaccatt atttccacca ccatcaggag
     3961 gagtagtaga gccaccacca ttgtttccgc cattatcagg aggagtagta gagccaccac
     4021 cattatttcc gccattatca ggaggagtag tagagccacc accattgttt ccgccattat
     4081 caggaggtgt agaaccgcca ccattatcac ctgtgttatt attgttatta ttattttctt
     4141 ctggttggtt attgtctggt ttttttgtat cttcagatga tggttgagaa cttactttag
     4201 taggagcagt accttcaata aatgctgctt tagagccaga agattgcttt cctccatcct
     4261 ctaaattaat ttttgtaaaa tatacatttt taggaacttc aaattcagta accgttaaat
     4321 tagcatgtat tttttccata attttttccc atagttttgc agtactagta cttccatgtg
     4381 gtacttctct acgttttata gtgttaccag aatcatcttt tctaccagca tcatctccaa
     4441 tataagttgc accaacataa taaggagtgt aaccgacaaa ccaagcatca tatgcactat
     4501 ttgtagtacc tgttttacct gctacaggca ttccctttgg aagtgtagca acaccaccag
     4561 taccttcagt aattacgctt tctaacatat ctgtaacaac ataagctact tcagggtcta
     4621 caactttatg ttcttcagga gcatttttaa ctaataattg accatcaaag gtagttatag
     4681 tagtaaaaat agaaggttct acataaatac cgtgatttgc caatgtacca taagcagcag
     4741 ccatttgaag tggagtaata ccattcgcca taccacctag tgtcaatgat gggaattttc
     4801 tatcacttgc ttcagatgaa cctgagtgat tattctttaa agttgttata ccaaagttct
     4861 ttaagtaatc catcattata tctacacatt catcataaga gtctccaagc atttcagcag
     4921 ttttgacagc tatcgtgttt gaagatttag caagtgcttt acgtaaactc atagaaccat
     4981 gaccagcagt agtagttctt ggattccatt tattattttc ttcaaactta tatcctcctc
     5041 gagaatcact tagagcagtt gcttgtgtta tttttagagt atcaatagct ggcgtatata
     5101 ttgctaaagg ttttatagaa gaacctggct gatgagggtt agtagcacga tttaaagttt
     5161 ttctaccact tatatttctt cctccagcta gtgctcttat ttcaccattt ttataatcta
     5221 agattaccat tgcagcttgc ggttggctaa ctccatcagg accgaccata tgtcctggga
     5281 aattgctatt tctatcaaat tcttcttcta aagcatcttg cattttaggg tctatagttg
     5341 tgtttacaat tagaccacca ttgtagaata agttttgagc ttcatcattt gtatgtcctt
     5401 gttccattaa agcatttata acttcacttt cgataaggtc ttctacagat gaagaaactt
     5461 tatctgatga ttgaggcaat tttatttcta ttttagcttt gattgcttca tcatattcac
     5521 tttgttttat atatttcaat tctaacattt tctttaaaac aatttcttgt ctttttttag
     5581 cttcaggatt acttacagcc tttctaacaa ctaatgtacc tgcttttaat tgtctataag
     5641 tatcattaga tataagctcc caagatttta ttttttcaat catatcaaag tccacttgag
     5701 tagggtcatc taaatcatca tcagttgtgt taacaaaaaa taataattta ttctctaaat
     5761 cctccttagt ttcatttccc tctaacttag aagttttata ggctgaaaat cttgatggat
     5821 ttttagttga accagcaaga agtgctgact ctgctagagt aaggtcagct gcagacttat
     5881 caaaataacc acgtgcacca gcctcagcac ctgctagacc tctaccaacg aagaagttat
     5941 ttaaataagt ttctagtatt ttatctttac ttactgtttt actcatctca tgtgcatagt
     6001 aaatatcctt tattttacga ggtatagttt gctgttcagt tgttaacagc attttggata
     6061 cctgcatagg aatagtacta ccaccttgct tagtacctgt aagggtcttt aatactgacc
     6121 taaataaacc tttaatatcg acacccttat gctcataaaa acgctcatct tctattgcaa
     6181 ttacagcatg ttgaagataa ggggaaatct tatctaatgg aacgggtgtc tttttattta
     6241 cacttggagc tgtagataat gtttttccat tagcgtattt aatagtagtt gtttggttcg
     6301 tctgtttatc taaaacagct tctgtaactg gagttacatc tcttagtgac gcaaatacta
     6361 gaccagcagt acctgctgta ccaacaacta gtaaaactaa aaatactatc ccaaatactc
     6421 ttagtttttt aaatttatcg ctttttttag attttttctt gttttttgtt gaacgagtct
     6481 tgttagctga agttctatta attggtttat ttgtattacc agatgagcta actttttttc
     6541 tcctgatttt atttccattt ttatcattat cgttattcat aagattttaa cctccttact
     6601 taatgcaata ttataacaca aataggagta gttttgtaat taatagaaaa aatgaatttg
     6661 ctataatatt ctgtacattg tatatgtata aacgtaatat attaaaaagt ctatattttt
     6721 attttattta tttttgacaa atattataaa aattatgcca aagctacata taaataaata
     6781 ggaggggata attttgaata gaatagtaga tagaaaaaca aaaaatgaat ttagaaaaat
     6841 catagctatt tttttagtaa ttcttttctt gtctgtattt ataggcagtt ttatatatat
     6901 agataaaact ttaagaccga caataacagt tttagcagag actaaagctc ttgaactagc
     6961 aaataggtcg ataaataaag ctgttgctga gatggtagaa ggaaaaataa attatgaaga
     7021 tttaatggat atacaacttg ataataatgg taaaataact atgatacaag caaacactat
     7081 aatgatgaat gaaattgcat ctgcaatagc tttagaaata caagatgaat taaaaaagga
     7141 taagacagca agttcttaca tacctatagg aacagcatta ggaagtccta tattagctaa
     7201 atacggtcct aaacttgaag tatctattga acctatagga acagtatcag taaactttaa
     7261 aacggaattt gagtcttcag gtataaatca aactagacat agaatatatt tagaagctca
     7321 aacacaagtt aaagtagtaa taccacttat aacttcaaca aaacaaatta aagctcaaat
     7381 acctatttgt gaaactataa tagtaggtga tgttccagaa agttatgtaa atattccaga
     7441 aaaaaatcta ggaaatgttt tacctaacac tggaaagaat accaacaagt aatgtaaagt
     7501 tgtatatttt taaataaatt caaaataata gtttataaat aaaatttttg gaaataatat
     7561 taatataatt ttaataaaaa tttatatttt atactatgta aaatatctaa tatatttggt
     7621 ctgtaggtta aataaaagta cctttagacc aaatatatta ttaataactt aataacatct
     7681 taaaacttat ttatttatat tatttacaac atccatttaa taaatacacc ataacctaca
     7741 gctgggtcgt tttcaattgc atgtgacact gctccgatta ttttattatc ttgaactatt
     7801 ggagtaccac tcataccttg tacaatacca ccagtttgag ctaaaagctt tttatctgta
     7861 attttaattt taaaactttt actttctgga tgtttttggt tttctatatc tataatttca
     7921 atattatatt ttttacactt attaaagtta tcttgtaaaa ttatttgagc ttttcctaat
     7981 ttaacttcat ctaaagatgc tactttatat ttttttaact tggaaatatc aaagttatta
     8041 atttgacctc ttattccaaa atcagtattt tctgtaaagc taccaataat attttctcgt
     8101 tttgcattta tacatccaac actaccttta taagattttt ctatattaag gtgcttagtt
     8161 gtagaaattg aacctgtttt aataggtatt tttcttgcat taccaacact tattggatga
     8221 cctacagctc caaactctga tgtatctgga ttgatatacg ttaaagtagc aaatccagaa
     8281 agtaaattgt taaaactaat tttttctaat atgtgtttgg tagttttcat tgttataagt
     8341 tgtccacctc tatttaataa aatagatact tgtttttctt ctggtaaagc atttaaggta
     8401 ttggcaaagt cactatatcc atcaacttca gcattattta cctttactat agcatcaccc
     8461 aatctaaatg gagatgcttc tccaggattt ctaactatga tattttttaa ttctgcgtca
     8521 atttgaagta cgtttccaat tggtattaaa tattcaggtt catttttttg taaattttgt
     8581 gcataaatta aattatttga gaaaaaatat aataaaaata aaaatgttaa aacaattgta
     8641 aattttgact tagctaagtt ggttttttta cttaaagtat tatttttaaa atgaaaattt
     8701 ttaagttgca taaaacacct ctttcttatt aaattatttt aataatagaa ttgaagtttt
     8761 cttgctgctt ctatattatg ttgtgcaaga atcatgtttt gatgcttgga aaactagctc
     8821 aataacacct tagttttcat aaaaataaat aaaataaata gtgtatatga taaaataaat
     8881 acatattcat ataaataaaa ataaacaata actgtctagc ataagaaagg agcaaaaaat
     8941 gaaaaaatat agattgttag ttacttgtat aattgccatg ggaatcataa ttataggaac
     9001 atcaaatcca gttagcaatt tttacaagac tacaaatttg attagttcag gaaatatcac
     9061 aaatgaaaaa gcagataaag atgaaaattc aaacaaaaat aataaagata aaaatgaaga
     9121 caaatctaat tctaaaagta ataaagataa gtcatcagag gacaaatcgt cagataataa
     9181 aaatgaaatt caaaaaaata aaaaactttt aatttgtata gacccagggc atcaaggaaa
     9241 aggagatagc aatttagaac cagtagctcc aggttcttcc tctaaaaaag cgagagtatc
     9301 ttcaggaaca gagggtattg ctacaaaaaa gccagaatat gttttaaact tagaagcatc
     9361 tcttgtttta aagagtatat tagaatctaa aggttataat gtaataatga ctagagagac
     9421 acatgatgta aatataagta attcagaaag agcaatactt gctaatgaca aaaaagcaga
     9481 tatggttgta agaatacatg cagatagttt aaataattct tcaaaaactg gagcatctat
     9541 attgattcct gaaaaagatg gaaaatatac agctcctata tatgaagaaa gtaataaatg
     9601 tgctgaattt atcaaacaga atatggaaca atcaggtata caaatcaatg gaatcgttca
     9661 aagaggtgat ttgactggat tcaactggtc aaaagtacca gctgtattag tagaaatggg
     9721 atttatgagt aactataatg aagaccaaat gatgtcaaat ccagactacc aaagaaaaat
     9781 gatgcagtgt atagctgatg gattggatgc gtattttaaa taagatatga atagagaaaa
     9841 agttaggttg gtattatttg cctaattttt atctctattt tttattgtac ataacacaaa
     9901 gaagaagttt attttaggtt aaattgtcgt aaatataact aaaaatattg ggaatacttg
     9961 atttaagttt aaaaagaata tattatagta tagaatgtgt aaaatttttt tgagaggtgt
    10021 tgaaatgcta gtagtagaaa atgtgagtca tggatttggt gcgagaacaa tattagaaaa
    10081 tgtatccttt agacttagaa aaggtgaaca tattgcacta gttggtgcta atggagaagg
    10141 aaaatcgagt tttttaaata taataacaaa gaagcttatg ccagatgctg gaaatataaa
    10201 atggtcttca agagctacag taggttactt agaccaacat acggtattaa gcaagggaaa
    10261 aactataaga gaagtgctta gagaggcttt taagcatatg tttgacttag agcaagaaat
    10321 gattgctatg tatgacaaaa tgggagaagc tagtgatgat gaaatgagta agcttcttga
    10381 agaaacagca gagatacaaa ctatacttga aaatagtggt ttttatatga tagatgcaaa
    10441 gatacaagaa gttgcaaatg gtcttggtct tggagaaatt ggtcttgata aagatgtaac
    10501 tgacttaagt ggagggcaaa gaacaaaagt acttcttact aaacttctac ttgaaaatcc
    10561 aactattctt atattggatg agcctacaaa ctatttagac gaagagcata taacatggct
    10621 tactaagtac cttcaagagt atgagaatag ttttgtctta gtttctcatg atattgaatt
    10681 tataaataat acatgtaatg taatatatca tatggaaaat ggagagttaa atagatataa
    10741 aggaaattat gatgaatttg taagacttaa tgatataaag aaacgtcaag aagaacaagc
    10801 ttatgataaa caagttgaag aaagaaaaag actagaggat tttgtagcta gaaataaagc
    10861 tagagtagca actagaggaa tggctaacag taggcaaaag caacttgata agatggaaat
    10921 attagaaaga ccaaaagaaa aaataaaacc tacttttgct tttaaagatg ctagagcagc
    10981 tagtaaaata atttttgaaa cagaaaattt agttttaggg tatgatgaag cattgacaaa
    11041 accacttaat ttccatttag aaagaggaaa aaagattgcc ttaaaaggaa tgaatggtat
    11101 tggaaaatct accctattaa aaactttact tggaataata aaaccatttg aaggaaatgt
    11161 taaattaggt gactatttgg aagttggata ttttgaacaa gagagttcaa gagaaaatag
    11221 taatacacct atggatgaag tttggagtga atttccaggg cttacaaact ttgaagttag
    11281 acaggctctt gcaaaatgtg gacttacaaa tgaacatata acgagtcaaa tgagagtatt
    11341 aagtggtgga gaagctgcta aggttagact ttgcaaagta atgttaaaaa atataaattt
    11401 cttagtatta gatgagccta caaatcattt agatgttgag gcaaaagatg aattgaagaa
    11461 ggctatcaaa gagtttaagg gaacagttct tttagtatgt catgagccag aattttattc
    11521 agaaattgtt gatgatgtat ggaatataga agattttaca actaaaatcg tgtagtgatt
    11581 taacttattg ctggggggat ttcatgaaaa agtttctaaa tgtacttttt tcaatttgta
    11641 ttagtattgt tataatagta ggtgttatta attttactgt tggatttaaa caactttatt
    11701 attttgacat tgattactta aatatatcag agttatctgg tttatctaaa gatgatataa
    11761 aattgaacta cgattatcta attgattata acttaaataa gaatgtgtct gagttcaaat
    11821 taccaacatt aaaatcttct ccacaaggga agatacattt tgaagaagtc aggaatatat
    11881 ttcaaaatat aaataaatta gctaaattat tattggtagt ttctttagta ggaattatat
    11941 tgagtgtaaa aaataaaaat ataaaaatct taaaaactac atcaataaca cttataatta
    12001 tgccattatt attaacagtt ccaattttat taaattttga aaagtcattt attatttttc
    12061 ataaattatt atttagaaat gattattgga tatttaatcc cgatttagac ccagttataa
    12121 atatattgcc agaggaattt ttttttcact ctggaatgat gattttaata ttaattttgt
    12181 tagtaagtat agtactgttt gtaatgtata aattatgtaa atctttatca ataaaaatat
    12241 ttcaatgtta aatagaggga tatttttatt tccaaaataa gataataaaa aaggattaga
    12301 ttaaaagtta tacaataaaa atagctgtgc aataatattt agactatgaa agtagttgaa
    12361 aaatatttat gcacagctat ttataataga atttactttt tactagtgat tttaactaaa
    12421 tcttgaaatt tactcataat gtaattgtaa ttatcttttt tatgaggtaa tatacaacta
    12481 ctgcaatctt tgataccttt atctgtatac ctaaagtttc caccacagtt ttctcctaaa
    12541 gcatacaaag gacaatagca aaatagacag ttaaactctt ctggtttatt agttttatga
    12601 caagggaaaa attcgcaatc tttgtgatta aaaaacttat aattttcgcc catattttcc
    12661 tcctaattaa ataaaattta aattttttat actatataag attttggtga taatataatt
    12721 atactaaata attttatgaa tagtcgataa tatagtatta ttaatcatag tattaatggt
    12781 ataattagtc aacagaaaaa taatgtaaaa ataatgtaag tgtattttgt aattagaata
    12841 tgttttataa caaaaaatga ggtgattaag tgaaagaaat tcaagaaaaa gcacttcttg
    12901 taggtctgaa tttgactaca atagtgaaaa aaaatgatga tatagacact aatgaatcta
    12961 tggaagaatt aaaagaacta acaaaagctg ctggggcaga agttgtaggt agcttaattc
    13021 aaaataaaca ctctgtagat gcagcttatt atataggaaa aggtaaagta gaagaaataa
    13081 gagcctatag tgactcttta gatgctactt tagtaatttt taatgatgaa ttatctggtg
    13141 ctcaaatcag aaatatagag aatgttgttg gaagaaaagt aatagataga actactttaa
    13201 ttttagatat atttgctcaa agggcactta gcaaagaagg taaattacaa gttgaattag
    13261 cacaattaaa atatagattg ccacgacttt atggaatggg tggtgagatg agtagaacag
    13321 gtgcagggat tggtactaga ggtcctggag aacaaaaact agaaatagat aagagacata
    13381 tacttaataa agcagcagat ataagacgtg aacttaaaga agtaaaaaag aatagagaga
    13441 cacagagagt taagcgttta aagtcaaata taccgattgt agcacttgtt gggtatacaa
    13501 atgcaggaaa atctaccctg ttgaacgaat taataaagac acacaaggat tatgaacaag
    13561 aaaaagaagt ttttgtaaaa gatatgcttt ttgcaacttt agatgtaact ttgagaaagg
    13621 ctcttttgcc gaataaaaaa gaatttttag tagtagatac tgttggattt gtaagtaaac
    13681 ttccccatga tttagtagaa gcgtttaagg caacattgga agaagtacag tatgcaaatt
    13741 taatcttaca tgtaattgat gcaacaaata ctagttatga attgcaaaaa agtacgactg
    13801 aaggtgtatt aaaagaactt ggtgtaaatg ataaaaaaca tatacttgta tataataaag
    13861 tggacaagct agaattggat atatacccta aaagtcaaga agatattgtg tatatttctg
    13921 caaaacaagg cataaatatg gataagttat taaatatgat agaaattgct ttaatggaaa
    13981 atacttattc agtaagtttg atgttacctt atgagagagg tgatatattt agtagaataa
    14041 aagataaata taatgtggag aattttgagt atggggaaag tggtataacg ctagatgcag
    14101 atttagatga agaagatttt aatatctata gagagtacat actagaaaaa taggttgaat
    14161 atggatataa actgggaaaa tatagataat cttgaagatt attttataac ctatcttctt
    14221 tataaagaat ctaaaacagt atctcaaatt agtaaaatta gaaatatttc aagtactgaa
    14281 gcaagtgagc aacttataca agcaaagtta aaaataaagg aaatgcaaaa agatgatttt
    14341 gaggcatcta aggatatttt ggataaattt ctagaactag ataaaattaa gagacttgat
    14401 tttatggata gcttagatga cgaaaaaatg gtatacttta agagaaaggt tttcaaaaga
    14461 atccttgtag aaaaaaatgc tgaagattta attgttttaa tttgggcgac aggtgagcta
    14521 aaagatgata ggtttttgaa attgcttcat cagctcacaa accacagaca ctcagatatt
    14581 agaagaataa cttattcagc aatcagaaag attgaatcac caagtagtag agaagtactg
    14641 caaaaaggtc tctacgacaa aaatgcacag actaggcagt actgtgcaaa agctctttca
    14701 aaattgggag atgaaaacag tttaaagatt ttacagcagt tgaaagacaa gaataagaat
    14761 tttgaaaaag aatatgtgct tagagcttat gacgaagcca ttaagtcatt ggaaaatgca
    14821 aaaaatttga aaaagtaatt ttttattaaa gcaatttgtt tacttaatga tgtatttcta
    14881 aagtttaaca aggtaataaa taaattaatg ggaggggttt caaatgatag taagacgttg
    14941 tgcaggtgga gtagtgtttt atgctaataa agtacttatt gtaaaaaatg ataggggtga
    15001 atggacactt ccaaaaggaa agatactagg agggggactt ccatatgaga gtgctgttca
    15061 aagagttaaa gtagaaactg gcatagatgc taaaatgata gatgtggctg gcgatactat
    15121 gtatgaattt ttctcaagaa gtagacaaca agaagtttgt aatgcaataa tgtggtatgt
    15181 tatggaagct tgtaacactg attgtgtact tgctcctgaa tttcaagaag gtggatttta
    15241 taaagtaaag gatgctttag aaatgttatc acatcataaa gagcaagctt tagtagaagt
    15301 atcttataaa aagtttaagg aattaaagaa attagtttca gagggcgatg atgttccagc
    15361 aaattaattg tgaatttaga ggattctgag gaagggatag ccccttcctt ttttaattga
    15421 agcagtttta agcgtaaatt atatagtctc tttaaactat gatttatcaa tttagagatt
    15481 tagtagttta tatttaaaat cagtaagata tatttaaaat aaaaattaaa aagtatacaa
    15541 ttttattata taattgtctt atgatgttga ttaaagaagt attaaaagtc catttatttt
    15601 taaatagaat gattttataa atatatattt gatgtgtata ttagaaaatg attaaaatag
    15661 gaggaaatac aatgagcaat tacaggacat tacatgaatt tggaacggat gaaataacga
    15721 ttgaaaagtc tgtttttatt ggatatgcta agcctataaa gagtgaagaa gaagcattag
    15781 agtttataaa tgaaataaag aaaaagcata aagatgcaac tcataatgtg tgggcttata
    15841 ctgtaggtaa aaatatgaat atacaaagat atagtgatga tggagaacca caaggaacag
    15901 ctgggatacc tacattagag gttataaaaa aagaagattt gagagatgta gcggttgttg
    15961 ttactaggta ttttggagga acaaaattag gtgcaggtgg tctagttaga gcttatacaa
    16021 aaggtgctaa actgggactt gaagcaggta agataattta taaggtaatg tatcaagaag
    16081 ttaaggttaa gatagattat actcaacttg gaaaagtgca aaatgagtta atgaatttgg
    16141 ggtattttat aaaagatact gtatatgagg ataatgttga aatagtagta tattcaagat
    16201 tagaagatgt agagaaatta tcagaaaaaa tgattgatat aacaagtgga acaggaaaaa
    16261 tagttttagg agaggaattt tatttgtctg aacaaaatgg agaaatttta atataatcta
    16321 aaaggttatt agaatagtta atacctcaaa tagccaatta gtatttttaa gtgaaaagcc
    16381 attaaattga tttaatctaa atcaatttaa tggcttttta tcggattttg cacagaatcc
    16441 tttttttact tcaaataaac cgactaaaag taaaaatatc ccaaatattt tttttaatat
    16501 atctgaagac agataattag ctaatagtga accaaataca gctcctaaaa ccccaaacac
    16561 agctataggt ataaccaatt taaaaattac atttttattt tttatatgta tagcaagtgc
    16621 tataagtgtc atagggatag atgaaagtaa atttacgctt tgtgctattt tagtgtctat
    16681 gccagcaagt aaaacgagtg caggtataag gattgttcct ccacccattc ccattcctcc
    16741 aataattcct gcaaaaaatc caattagagc aaataacatt aaaacaccat ccttaatgct
    16801 gcaattatca taattattcc aaaagaaatt ctaagaaact ttccagtaac cttatttagt
    16861 aatttagctc ctataatacc accaagaata cttccaactg caactttaat tgttaagtta
    16921 acatcataag tacctttaga tacatataaa acagaacttg ctgtcgttaa gaatattatt
    16981 atagaaagag cagttgcatg agatttatgt tcttctacct taacgatgtc atttaatatt
    17041 ggaactaaaa gagtacctcc tccagaacca aaaactccat ttataaatcc tgtaaaaact
    17101 ccaattatcg tattttttaa attaaaatca aagtattttt tctttaaatc atcagttttt
    17161 ttcataaaaa tcaccttctg aaatagtttt accaaaatag gcaaattata tttatataaa
    17221 ataagattaa atataatctt tcatgtttgt ttaattttgc ataaaaaaaa acatattaaa
    17281 acaacatatt aatatatggt ataatatatt aaaacaaagt agcaaatttg agaggagata
    17341 acaatgtcaa atataaaaat acaaatagac aaaactgtag aatggcttat aaataaggtt
    17401 aatgaggcaa atgcaaaagg attaattgtt ggtgtatctg gaggaattga ttcggcagta
    17461 gttgctaatt taattaaaaa ggcatttcca gaaaactcta tgggagttat aatgagcata
    17521 aaaagtaatc ctcaagatag agaagatgca cttaaggtta tagagggatg tgatatagaa
    17581 taccttgatt tagaccttat agaaccacaa agtgctatat tagacatggt agtaggaaat
    17641 ttaaaagata aacatttata tagagaagaa tatttaaaga tgacagatgc aaacttaaga
    17701 gcaagagtaa gaatgagtac aatttataca attgctaaca atctaggata tctggttgtt
    17761 ggtacagaca atgctgcaga aatacacaca ggatatttta ctaaatttgg tgatggtgga
    17821 gttgatattt tacctattgc aaatttaact aaaggagagg tgtatgaatg ggcaaaagag
    17881 cttggtgttc atgaagattt aataaacaaa gctccttcag caggtctttg ggaaggtcaa
    17941 acagatgaag atgaaatggg aactacttac aatatgatag atgctgtatt agaaggtaga
    18001 ttggatgaag ttcctaaaag agaccaagag ataattgaaa gacttcatag attaagtgaa
    18061 cacaagagaa aaacgcctgc acaacctcct aagttctaat ttactattta taagatttgt
    18121 gataaaattt acaagtgatg aaaattagaa taattaattg tacgtattaa ggagtggaaa
    18181 tatgggacgt ataggtaata taattaatag aaaaggtaaa caagatgctc aaagagcaaa
    18241 gatatttact aaacatgcta gagcaatagc agttgcagct aaggaaggtg gagcagaccc
    18301 agagtacaat gcagcgttaa aaacagctat agaaaaagct aaggcagata atatgccaaa
    18361 tgataatata gatagagcta tcgctaaagg tgctggagca ggtgctggag aagactatga
    18421 aactatagtt tatgaaggat atggacctgg tggagttgct gttatagtag aaactttaac
    18481 tgacaacaaa aacagaactg ctggtaacgt aagatactat tttgacaaaa acggaggaaa
    18541 cttaggaact agtggatgtg tgtctttcat gtttgataaa aaaggacaaa tattagttgg
    18601 tttaggagat ggagtttcag aagaagaatt gatggatgtt gcattagaag caggagctga
    18661 agatttcata acagaagaag atggttatga aataataact acaccagaag atttttctag
    18721 tgttcgtgat gagttaaaag ctaaaggata tgagtttata tctgctgatg tgaagatgat
    18781 acctcaaact acaactgtat tgactgaaga atctcattta aaaatgatga ataaattagt
    18841 tgatatgctt gaagaagatg atgatgttca agatatatat cataactggg aagtagaata
    18901 aatagtataa gcatattgaa atttataagt attgaatcaa ttttaacatg tgataaattt
    18961 tatagaataa gaaacactaa aatagacata cgcacctcta acctatattt gcataataat
    19021 atgtaagcat aggtaagggg tgtttttata tttataggtt aagaaattta aagtaaattt
    19081 tgagctggac tcgattgtct ataaataatt gatgggtata taacttgttg ctgaatacca
    19141 tttatacgcc cagttactgt atcaataatc atcctaagac tacgttctgc tgtctcttcc
    19201 atttttaaat ctataactgt caaaggagga atacttcttg ccattagtcg tgaaggtcca
    19261 ttacttgctg caaatatttg aatatcctct ccaattacaa ttcccgattc atgaaatgca
    19321 ttcatcatac caagagctac cacatcatga ttacaaaaaa ttactttttt tagtgaattt
    19381 tttgcaagca ttttttgagc aatatcatat ccactatcta ttttattaga acaataatat
    19441 atagaatctg atagatgtat tccatgttct ttacatgttt ttaaaaaaga tttacccctt
    19501 aaagtcatac atgtaaagga agttgggttg aatacaacag caatatcatt tttaccattt
    19561 tgaatagcat gtaaagctgc tatacgacca gtttcctcat tatcaataga tacacttgaa
    19621 aaaccaggta tttcacgatt tacaacaact acaggaattt taggtttatt ttttactaga
    19681 tattctatat catcagcatt gatacctgtt aaaacaattc catcataata attatttgca
    19741 aaaaagtatt catcatcaca tagatgacca actttgtaag gtcgtatgat gatatttatg
    19801 tttaaatgtt ctaaagaaat tatgtggttt attgcttgaa gtatattggt tgatgccaac
    19861 tcaaatccct catcctgcca ataaaaagct attttaatat tattatgttt aataggagta
    19921 gagcgtttat atccaagctt atcagctgaa tttaatataa gctcttgtgt atgtagcgga
    19981 atcctgcgtt catttccttt accacttaag acgtacgaga cagttgctga agaaagacca
    20041 cagtcttttg caatatcttt gattgttgcc atgataaaat cctccttaaa tagttgctta
    20101 aatttgctta taccaataat cttaacatat attcaactaa agaacaatat tctacaaggt
    20161 gcatatttta caggattaga ataaataaat taaaaaaaac ttcttactta tgttgacata
    20221 atttagttta taggattata atatgcttaa ataagcttat gattaacttg aattaagcaa
    20281 agttaagcag gaacatgcaa tgctagcttg caatgtgact gcaaaggggg aaaaggtatg
    20341 agtaaggtaa atatatttga agttggtcct agagatggat ttcaaaacct gaaagagttt
    20401 ttagaaactg ataaaaagct taagattatt gatggtctta ttgcttctgg gattaaacaa
    20461 cttgaaatta gttcatttgt aagcccaaaa gcaatacctc agatgaaaga tgcaaaagaa
    20521 gtagcaactt attgtgtaga aaaatatcct gatgtgaaac tttatgcatt agttccaaat
    20581 ttatatggtg ctaaagttgc atgggagtgt ggtataagag atatatccta tgttatatct
    20641 ttgagtgaaa ctcataataa aaaaaatata aatcgtaccc atgaagagtc tattgatgag
    20701 cttaaaaaaa taatgaatac ttatccagac atgaatatat gtattggaat ggctactgta
    20761 tttggatgtc cttttgaagg tttaccaaaa attgaagatg ccataagatt tgctaaacgc
    20821 ctttgggaaa acggtatcag ggagataaat ctagcagata caatagggat tgctgaccct
    20881 aagcaagtaa gagaaactat tagtgcatta gttgaagctt taccagaatg cagatggcaa
    20941 gtacatattc atgatactag aaatatggga atggttaata ctcttgtggc tattgaatct
    21001 ggaattacta ctgttcagtc tacacttgga ggattaggag gatgcccatt tgctcctgga
    21061 gcatctggca atactgcaac tgaagattta gtatatatgc ttgaacgtat ggggtatgat
    21121 acaggagttg attttgttaa acttcttgaa ttatcaaaat atcagaagag ttttgtaaat
    21181 ggagtatata gtggtcatca tgtaaatata aagtctgata gttgtaattg ttagttctat
    21241 tgattaatcg attaaaaaaa tatgctgatt ggtaaaatag atttattgat aaaaaatata
    21301 aattataaaa tacattatct aataaaacaa ataaaaaaat tgagggggat tagtatggat
    21361 attatagctt tacttttatt tttaggagtt attattattg catttgtacg taagaataat
    21421 gcaggtattt tggctctagc tattggtgtc atagctgtta gaatttttgg aatggatgat
    21481 aaagaacttt atggagctat aagcaactca atgtttacta cacttgttgg tataacacta
    21541 ctctttgcaa tagtgaactc aacaggagca ttaaagcttg ttgcagaaaa aattgtagct
    21601 gcttctggta aacgtgtttg gcttatacct atagcagttt ttatagcagg atttgttgtt
    21661 gctggtgttg gacctggggc aattccagca cttgcaatta tcccagcaat ggctgttcct
    21721 gtagccataa cagttggtta taatcctgta atgcttgcac ttataggtga atgtggatta
    21781 atggcaggtc gtatgactcc tattactcca gaaggtcaaa ttataacaac cgttgcagaa
    21841 ggggttggaa ttgaaaatgt tatgcctact atcttagcct gtcaaactct ttctacaatt
    21901 gtattttcaa tagtattgtt tataattttc aaaggatata aacttaaaaa acctataaat
    21961 gttatagata gtaagcaaat agaaaaattc aatataaagc agataatttc tctaataagc
    22021 atagtagtta tgattgcttt tatcataatt tttgatatga atgttgggct tgcagctttt
    22081 gcagtttcag ctgtacttat ttttttcaat attgctgatg aaggagaatg tatcaaatca
    22141 attccatgga gcacgatagt aatggttctt ggagtaggtg caatgatgac aatcgtagat
    22201 gcagcaggag gtattgactt gatgagtaat gcattatcat cactgatgaa tagtaaaaca
    22261 gctactccaa ttatgagtat ttcagcagga cttatgtcta tggttagttc tgctttagca
    22321 gtagtttatc caactatgat gcctatgtgt gttgacatag ctaaggcagt tggaggagta
    22381 gacccactag cacttattgc tgcagttggt gttggaggtt ctcttgctgg tgtaagccca
    22441 ctttctactg gtggggcatt gattttagca gctatgggtt caagtaagaa agactttaat
    22501 aaggaagaac aaaataaggt ttttgtacag cttttcatta tgagtgcagt tggcttattg
    22561 gttattgcag tagtatcaat attatcattt aatgcaataa cacatttaat gaactaatta
    22621 gaataactga aaataaatca ttaaatttaa cttttaatat taatcaatat aatataattt
    22681 ggaggggaag ttatgaaaaa aggagcatta agcaatataa aggtacttga ccttacaaga
    22741 gttcttgcag gtccatattg tacaatgatg ttggcggatt ttggagctga tgttataaaa
    22801 atagagatgc caagaaaagg tgatgataca agaggttttg gaccttttgt aaatggagag
    22861 agtttatact atgcaaatat aaatagaaat aaaaaaggta tcactctaaa ccttaaatca
    22921 gaagaaggaa aaaatatatt tttagaaatg gtaaaacatg ctgatgttgt agtagaaaat
    22981 tacagaccag gtgtaatgga aaagttaggt ctgggttatg atgagttggc taaagttaac
    23041 aatcaaataa tttatggagc tgtatcagga tttggatgtt atggaccata ttcagaacgt
    23101 ccaggttatg atattattgc acaggcaatg ggtggtctta tgagtataac agggcaagaa
    23161 ggaggagaac caacgagagt tggtaatgct atgggtgatg tactaggtgg aatgaattta
    23221 actattggta tactcacagc actcaacgct agaaaaataa taggaaaagg acaaagagta
    23281 gatgtttccc tcgttgattc tgttgtatcg tctctcgaaa ctggtataca gagatacttt
    23341 gattcaggac aacctgaact tatgggtaat agatatgctt cagcctatcc ttatgactct
    23401 tttaaggcaa gtgatggaag ttttgtaata ggctgtggta atgacaaact ttttgaaaag
    23461 ttatgtaaaa aagttctttt tagagaagac cttttaatag atgagagatt taaaacaaat
    23521 cttaatcgtt gtgaacatta caaggctctt aaaccagaaa tagaaaaatg gtctgttaag
    23581 catacaataa aagaagcagt tgacttaata tcagaggctg gagttcctgc tgctcctatc
    23641 tataatcttg attgtgttac aacagatgaa catatagcaa aagcaagaga aatgattgta
    23701 gatattagtc atcctattat aggaaatatg aaagtcaatg gaaatccagt taagttgatg
    23761 acaacaaaag ctaaaattga tatgccagca ccatctctag gggaaagtaa cgaggagatt
    23821 tataatggtt ggcttggttt tgatagtgat aaaataaaag atttaaaaga aaatggagtt
    23881 atttgattaa aaaatagcta aacacacttg tgactttagt catgggaggt tcagttagaa
    23941 taatatcaaa aaatattaaa tcaatactgt aaataaaata tagaattgtc agaagcattt
    24001 aagatgggaa aagctactta gacattgagt ctttgtggct tttcctattt tcattaagaa
    24061 ttttatatta tcagaataga tatgtactaa actaaagtat taacaattta aattaataga
    24121 aaataaacta cctaattatg ttgaataaag actactttag tgaaaataaa cttatagaga
    24181 tgctatttaa attattttaa tggaaatatc aaaaatgaga ctgcattctc aaaaatgaga
    24241 ctgcgttttt taagtatttt ctcgaaaatg agacaaaaat attgttttat gggttttatt
    24301 gaagaaaaat tcttctgtga attacaattt aatcaatttt aaagttgaaa attattaaaa
    24361 atttagtgaa atttatgtat atttcgttta ttttattaaa aataacaatt ttttactaaa
    24421 aataaagaac ttcaatggtg attggcatag tacttgctat tttatattaa caagaaatgt
    24481 agaaaaaata acaatgtata atggggggaa tatgatggaa tctgtaatgg aaaatttgaa
    24541 caatcttaag gtggaggtta aggacaaagt ctgtgttatt actataaata gacctaaagc
    24601 tttaaatgct cttaatagtg acactcttag ggaactatca caagttattg atgtagtatc
    24661 agaaaatgag gcaatattag gagttattat tactggagaa ggtaaagttt ttgtagccgg
    24721 tgctgatata cgccagatgc aaaactataa aagtgaggaa ggcagaaaat atgcaggtta
    24781 tgctcaggga atttttgata aaattgaagc tcttgagaaa acagtaatag cagcagttaa
    24841 tggatatgca ttaggtggtg gatgtgaact tgctatgagt tgtgatatta gaattgcatc
    24901 agaaaaggct atatttggac aacctgaagt taatttagga gttattccat gttttggtgg
    24961 aactcaaaga ttatcaagac ttgttggaac tggaatagct aaagagttaa tttttacggg
    25021 gagacaagta aatgcagaag aagctaaatc tattggactt attaataaag tggttccaag
    25081 tgatttactt ttagaagagt ctatgaagat gatgaaccaa atagttgaaa aagcgccaat
    25141 agcaataaga tatgcaaaag ttgttattaa caaagggata gatatggatt taaagaatgc
    25201 tcttgaacta gagaaagatg ttgcaggttt aacttttgct acaagagata agcaagaagg
    25261 aatgaatgca tttatagaaa agagaaagcc agtttttgaa aataaatagc tattataact
    25321 aaaattggaa caatgtagaa tatattcaaa atctaaattt cgtgaaaaaa tacatatagt
    25381 ttaattttaa aaggagttaa tttatgaata cgggagtaat tataagttta gtagggatta
    25441 tcatagctct atctcttcta gttgtaatgg ttatgaaggg aataaatatt tttattatag
    25501 caattgtatg ttcatcatta gttgctataa caggaagcct aaatgtgtat gatgcactta
    25561 aagtggatta tatgacaggg tttgttggat ttcttcaggc aaatttcttt attttcctaa
    25621 cagggacatt aatgggaaaa ataatggaga ttactggtgg agcaaaatct attgctaaga
    25681 tgattgtaag atggattggg aaggataaag cactactttc tattccaata gcttgtggaa
    25741 tattagccta tggtggagta agtgttttcg ttgtaagttt tgcagtgttt ccgatagctt
    25801 tagaaatttt taaagaagct gatttgccac gaagatttat accagcagct ttgacttttg
    25861 gatgctcaac ttttgctatg gttgcaccag gggcaccaca aattcagaat attgttccag
    25921 catcaacatt agggacagat attatggctg gagcagtaaa tggttttata tcatgtgcag
    25981 taatgtttat agttggaagt acaattttgt acaaaatggt ttcaaaagaa aaagccaatg
    26041 gtggtcattt tatagcacat gaatcagata tatttgatga agcagaagca actaattcta
    26101 agagagaaaa tggtcccaat ggtgtaattg ccttaattcc attgatagtg tcgattttaa
    26161 taattaatgt aaaagttaat ggaaaagcga tagtaccaat tgaggtggga gtatttattg
    26221 gagctttatt agtatattta ttgttaaata aatatcaaga taatactaaa atagttggac
    26281 atgttggtga tgcatgtaaa actacagtag tagcaatatg taatacttgt gctgtagttg
    26341 catttggttc agtagtaaaa tcagcagttg gttttgactt catagttaat gctatgacta
    26401 atattcctgg tcctcctatt gcaggggctg cagttggaac tacagttatt gcaggtattt
    26461 gtgggtcagc ttcaggtgga ctaggaattg cagctccact tcttggacca gtatttttag
    26521 ccaagggtgt ttccgctgca gcattaacta ggactatggc aatttcatca gcagcattag
    26581 attcacttcc acacaatgga tatattgtta ctgtaacaaa tggtctttgt aaagaaacac
    26641 ataaggatgc ctatatgcca atattctggg taactgtttt aaccccattt attggaacca
    26701 tagtagcagt cctactgttt acaatgttcc cgggattgcc ataggagaaa agataaaagg
    26761 tataaattat attttgatat tataaaatca attttcaaaa tatatacaat aaatttcatg
    26821 ttttaatact gtaaatatgt atttaaaata acaaattgac catataagga ggaattgaaa
    26881 gtgttaaata aaccattaga gggagttaag gttattgacc taacgtattt tgtagcaggt
    26941 ccagggactt ctaaaatatt agctgactgg ggtgctgacg ttattaaggt agagccaagc
    27001 tttggagacc caggaagaaa gacaggggca acaatgacaa tgccaataga tgattataat
    27061 aatccttttt acagcacata taattcaaat aaaagaggtt tgtctataaa tcttaagagt
    27121 gaaactggaa ttgaaattat ggataagctt ttaagcgaag caaacgtatt tgtttcaagt
    27181 tatagaacag gagcattaaa aagactaggt cttgattatg aatcattaag taaaaagcat
    27241 ccacacctta tctggggtca gataaatggt tttggcgatt ttggacctgc taaggataat
    27301 gcaggatttg atacagttgc cttttgggca agaagtgggg ctatgcttga tatagcagaa
    27361 aaagatacaa gtccaataaa tccacctata gcctttggag atgcaacaac ttcttgtagt
    27421 ttatctggag gaatatgtgc agctttgtac cagcaagcta agactggaaa aggacaaaaa
    27481 gtaatggcat ctttatttgg gcaagcaata tggaatgcat catctttatt agcatctaca
    27541 cagtttagtg atgagtatcc aaaaactaga aaaaatgcta tttcaccagt aataaattct
    27601 tatcgttgta aggatggaaa gtggatattc ttaagtattt tagagcatga aagatatttt
    27661 gaggcacttt gtagattatt tggaagagaa gacttaataa aagaagagaa atttgcatct
    27721 tcagtagaaa gtaagaaaaa tgctacagaa cttattaata ttttagatgg tgaatttgca
    27781 aagtttactc aaaatgagat ggttgaaaaa cttactgctg ctgatatagc tcatgaaaaa
    27841 attcaacatg ttaatgatgt agtgactgat gaacaggcta ttgctaataa ctatatatat
    27901 gaacatacaa gtaaagatgg aaataagaca atgcttgcat ctactccagt taaatttgga
    27961 aatattgaag ttaacatgac ttgtgatgct ccacttatag gagaacattc agatgagata
    28021 ttgagagaac ttggatacag tgattctgat atagagactc taattgaatc tggaattgta
    28081 acaatgaata aagaagcatg tgaaatatgt taataaacat gtgaagtatg ttaataaata
    28141 ggagggaatt ttatggattt tagattaaca gaagcacaat taatgcttca aagagtagca
    28201 aaagaatttg cagagaatga aatagcacca atagctgctg aaacagataa aacaggtatt
    28261 tttccaaggg agctttttag caaaatggct aaaataggat ttaatggaat tggaacacct
    28321 gtagagtata gtggttctgg tggggcagat atagaaaagg taattgttgt tactgagatt
    28381 gccaaaaaat gtgcagcatc tgcagcaata ttatcaatac acacaatata tgctcaagct
    28441 attttgaaat ttggtactga ggaacaaaag aaaaaatacc ttccaatgat ggctgaaggt
    28501 gggtgtgtag gtgcttttgc cttaaccgaa cctaatgctg gttcagatgc tgcgagagct
    28561 gcaacaactg caattattga tgaagaaact gatgaatatg ttttaaatgg aactaagtgt
    28621 tttatttctg gtggaggtca agcagaatca ttaataatat ttgcattgac agacccatca
    28681 aagggaataa agggaatgtc tgctatcatt gtcgacaaag gtactccagg tttttcaata
    28741 ggaaaaattg aagaaaaaat gggtattcat ggttcagaaa ctgcagagtt aatatttgat
    28801 aactgtagag ttccaaagtc taacttatta ggaaaagaag gtaaaggatt taatatagct
    28861 atgacttgtt tagatggagc aagaattgga gttggagctc aagctgtagg aattgctgaa
    28921 ggggctttag aagaaagtat caaatattct aaagaaagag tccagtttgg caagccaatt
    28981 tcagctttgc aaggtattca atggtatata gcagatatgg cgacaatggt ggaatcagca
    29041 aaattattag tatattatgc tgcagatttg aaagctagag gagaaaaaca cactaaagaa
    29101 gctgctatgg caaaatacaa tgcgtcacgt actgcaagag aggttacaaa tttagccctt
    29161 caaatccatg gtggatatgg atatatgaag gattatccat tagagagaat gtacagagat
    29221 gccaaaataa cagaaattta cgaaggtact tcagaaatac acaaagttgt tatttcaaga
    29281 gcagtactag gataggagga aaagaatgaa tatagttgtt tgcttaaagc aagttccaga
    29341 tactaatgaa gttaaaataa ataaagagac aggtactcta ataagagatg gtgtaccaag
    29401 tataatcaat ccagatgata gaaatgcttt agaagaagct ctaaaaatga aagatgaact
    29461 aggagcagtg attaaggtta taagcatggg acctcctcaa gctaaaagtg ctttaaaaga
    29521 agctttggca atgggagctg atgaagctta tttaatctca gatagagctt ttggtggttc
    29581 agatacttgg gctacatcta caattattgc tgctgctata gagaaggttg gaaagtatga
    29641 tgtaatattc tgcggaagac aagctataga tggagataca gctcaggttg gaccagaggt
    29701 tgcagaattt ttagggatac cacaagttac ttatgcgaaa gaagtaaagg ttcaagatga
    29761 caaattacta gtaactcgtt atacagagac aggggattat ttaatagaag caaagatgcc
    29821 agttttatta actgctataa aagagttaaa caatccaaga tatccaagtg tcaagggaat
    29881 cttagaagct tataataatg gagatgcaaa gattactgta ttaacacttg cagatttaga
    29941 tgttgataca actcaaattg gattaaaggg ttcacctaca aacgtttata agtcttttgt
    30001 accagttaaa gacaagcata atgaaataat agaaggtata aataaaaaag aaaaagctga
    30061 gaaactaata gaaatactat tcgatttgaa actagtatag gagtgttaag atgatgagag
    30121 caaaagttaa tcaaggtata aatttaaatg attataatgg tgtttgggta ataggagagc
    30181 aaagggaagg taaaattaat cctgttacga tagaacttat tggtgaagga agaaagcttg
    30241 ctgaccaatt aggaaaagag cttgcagttg tgatagctgg atatgaagta gagaaagaag
    30301 tgaaggaatt acttcactat agtgttgata aagtatatta catcaatgac cctcttctta
    30361 aagatttcac tactgatgga tatgcaattt caattgcaaa tttaatcgaa agaaaaaagc
    30421 cagaggttgt attggttggt gctacttcta taggaagaga tatagcgcct agaattgcag
    30481 gtaaggttgg aacaggtcta actgctgact gtacaaaact tgaaatagat tcaacagata
    30541 ataagttatt acaaacaaga ccagcatttg gtggaaattt aatggccact attgtttgtc
    30601 caaaaaatag accacagatg tcaacagttc gtccaggagt tatggcaaaa gctgtaagaa
    30661 acgaatcaga gactggaatt ttagaagttg ttactccaga acttactgaa aaaatgatta
    30721 gaactagatt agtagagata cttccacaag aaaagaaatc tgtaaatcta actgatgcta
    30781 gaataatagt atcaggggga agaggattaa aaagagcaga aggatttgag cttataaaag
    30841 aattggctga taaattgggg gcagagattg gtgcttcaag agcagcagta gattcagggt
    30901 ggatagaaca ttctcatcaa gtgggacaaa caggaactac tattagacca gaactataca
    30961 ttgcatgtgg aatatctgga gcaatacaac atttagcagg tatgagtgat tctaaatata
    31021 tagttgctat aaataaagat gcaaaagccc ctatttttag tatatgtgac tatggaatag
    31081 taggagattt atatgaaatt atacctgaaa tgatagaatc attaaatagg taacttttag
    31141 aaaacattga atatgtagaa tgataagtct tataatattg tattgtaaga cttattattt
    31201 ttgctgaagg ttatagtggg gagatgaaac aatgaaagag cagtggtata aggatatatt
    31261 tgcaagagta ttatctatga ctgatgatgg ttttattgtt gtaaatacaa gtggagtaat
    31321 aatagatatt aatgataaat attgtgactt tttaggaaaa gaaagaaaag atataatagg
    31381 acaaaatata caaagtatta taccaaatac aaaaatgcta gatgttatga aaaataagta
    31441 ttgtgaagaa ggagctatac atcattattc aggtggaaat acaaaagaaa aaagtgtaat
    31501 agtaagtagg tcttatgtgg aaaatgacaa tggagaagta gttgcaggag tagctcaagt
    31561 taaatttaga ttgcaatcat ttgatgtagc taaaaagtta atgtcagaat atatggagct
    31621 ccaatattat aaagagcagt ttaaggataa ctgtggtttt gacaagctca taggtgaaaa
    31681 tagagacttc atagaactta agaaaacagg tgtgaaagct tctaaaacaa actttccagt
    31741 acttttaacg ggtgaaacag gcacaggtaa agaagttttt gctagagcaa ttcacaataa
    31801 tagcagtagg tcagataaac ctatggttag tataaattgc gctgcaatac cagaagaatt
    31861 attagagtca gagctatttg gctatgacga aggagctttt acaggagcaa aaaagggtgg
    31921 taaaaaaggt aaatttttag ttgcgaataa tggtacaatc tttttagacg agataggtga
    31981 catgcctttg accatgcaag caaaactttt aagagttctt caggaaagtg agatagaacc
    32041 agtgggaggt cttaaaacta taaagataga tgtaagagta atctctgcca caagaaagaa
    32101 tttaagtaaa atggttgaag agggattatt tagagaagac ctatactata gattaaatgt
    32161 aataaacatt catatgatgg aattgaagga caggcaagat gatattttat tgctagcaaa
    32221 ttatatatta aacaaattaa atgttgaata caaggaattg aaggttttaa gtgataaggt
    32281 taaaaattgt tttattaact atacttggcc gggaaatata agagaactac aaaatgtaat
    32341 aaaaagtgcc tatgctgtaa gtgatgatat ggtcattatg atgtgtgacc ttccatctaa
    32401 gatggataat atcagcagag tagctcaatg taatgtggat agcaattgtt caattcatga
    32461 aatggttgag aattatgaaa agagcttaat tattgatgtg ttaaggaagt ataattggaa
    32521 atgttctaag gcagcagagg ttatgggaat acacaagagt cttttatata agaaaataaa
    32581 aaagtatgaa attgaattaa ataattaaga taacttgaat taaggcaatg ctaaaaaagc
    32641 gcccttattc aaagcgaact gtataaactg ttttatgcag ttaaaacgtg taaaagtaga
    32701 tgtgaagtac taatgtcaat cattagtact tttttattat atttataatt actatctata
    32761 tttcagtatc ttttgatatc taagattatt atgtttaatt tagtatataa caaaaccctc
    32821 ttagttatgg acagtttttt atattttaac tgtctactaa gaggggatag tttagtttaa
    32881 atttagagta aattttatct agcatttata aaaaatattt tgaatagctt ttttgttatt
    32941 tagtcataat tttctacttc tttatcaagt tctttgccac aagaatcaca cttatatata
    33001 gcattgtatc cgtaatcata ttcatcaact attttctttt gtctaaatac accaccacac
    33061 tcacatagga aaccaatttt tataagtgta acgataaatt gtgattttat aaattttata
    33121 tcattgcatt tatcgttagt gtaaaatgta taaatataag agttatcgca ctcatctctt
    33181 aattcaaatc tttcaatata tgtgttattt tttaattcct caaattcttc tttagttaaa
    33241 tatgattttt gttgcaattc atgtaaacgc ttaatttggt ccattattta tatcctccca
    33301 ataattttag tattttaagc ttagataagg aatctaagtt ttattaaatt tatcccaagg
    33361 aattatttaa atcatattta gttttattaa ttacaacata tataaattac acgaaaataa
    33421 aactaattgt aaaaataata tcctgtgaaa acctaaacta ctaacccctt actaacccga
    33481 aaactaaaaa aagaaaatat atccaaatat aagtgatata taaataaaga tgtttcatat
    33541 aaatttaact ttaaa
//
LOCUS       DAYAOX010000017        33288 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.017, whole genome shotgun sequence.
ACCESSION   DAYAOX010000017 DAYAOX010000000
VERSION     DAYAOX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.017"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            382..1203
                     /gene="yidA"
                     /locus_tag="ACGI57_000687"
     CDS             382..1203
                     /gene="yidA"
                     /locus_tag="ACGI57_000687"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFG9715774.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            complement(1339..1980)
                     /gene="saoL"
                     /locus_tag="ACGI57_000688"
     CDS             complement(1339..1980)
                     /gene="saoL"
                     /locus_tag="ACGI57_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_238476746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1495..1497),aa:Sec)
                     /transl_table=11
                     /product="MerB-like organometallic lyase SaoL"
                     /protein_id="HFG9715775.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     CUNIMNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLKQKVLS"
     gene            complement(1993..2745)
                     /gene="saoA"
                     /locus_tag="ACGI57_000689"
     CDS             complement(1993..2745)
                     /gene="saoA"
                     /locus_tag="ACGI57_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein SaoA"
                     /protein_id="HFG9715776.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            complement(2745..3488)
                     /gene="saoP"
                     /locus_tag="ACGI57_000690"
     CDS             complement(2745..3488)
                     /gene="saoP"
                     /locus_tag="ACGI57_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591060.1"
                     /note="Most members of this family are selenoproteins with
                     the selenocysteine residue at the channel-gating position;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2922..2924),aa:Sec)
                     /transl_table=11
                     /product="ABC transporter permease subunit SaoP"
                     /protein_id="HFG9715777.1"
                     /translation="MDILYKIILLVIIFLVWHFAAKDIGSSLLLPMPVDVIKGFFFCV
                     TDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFSKTFERVLSPVVDSVRQVPIMA
                     WVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVRAISKDYYNAARSMGASPIVIF
                     TNVIVPASLPDILTGSRIAISTGWMSVIUAEFIATSAGLGYSMVQAQTLMHTDVLIGL
                     MIFAALIGFIIDRVLKFINKILCRWRFAD"
     gene            complement(3512..4300)
                     /gene="saoB"
                     /locus_tag="ACGI57_000691"
     CDS             complement(3512..4300)
                     /gene="saoB"
                     /locus_tag="ACGI57_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4085..4087),aa:Sec)
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     (seleno)protein SaoB"
                     /protein_id="HFG9715778.1"
                     /translation="MKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTSGFV
                     INYMVNRDYFENIKVSDIMDIFTINDCUASTMQWALSSEEIDIAIICKDAAKQYVNVN
                     SGFEIVGTVVQNSDIFLLSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAY
                     GLESNLVDGVVIDCIKSIGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYN
                     KSVDALKNKATFKKVLKEYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            complement(4314..5405)
                     /gene="saoX"
                     /locus_tag="ACGI57_000692"
     CDS             complement(4314..5405)
                     /gene="saoX"
                     /locus_tag="ACGI57_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding subunit SaoX"
                     /protein_id="HFG9715779.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            complement(5440..5898)
                     /gene="saoC"
                     /locus_tag="ACGI57_000693"
     CDS             complement(5440..5898)
                     /gene="saoC"
                     /locus_tag="ACGI57_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Cys-COOH (seleno)protein SaoC"
                     /protein_id="HFG9715780.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            complement(5891..6976)
                     /gene="saoE"
                     /locus_tag="ACGI57_000694"
     CDS             complement(5891..6976)
                     /gene="saoE"
                     /locus_tag="ACGI57_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter SaoE"
                     /protein_id="HFG9715781.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            complement(7003..7260)
                     /gene="saoT"
                     /locus_tag="ACGI57_000695"
     CDS             complement(7003..7260)
                     /gene="saoT"
                     /locus_tag="ACGI57_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_240067706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7225..7227),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-like (seleno)protein SaoT"
                     /protein_id="HFG9715782.1"
                     /translation="MSKILVEFINTUPSCDEHGVFIKKMSEKYADKLEIKLYQAGKDF
                     SYIKKYGIITKGTLIINQKKKYDRLNKDTIERAIVEAINNN"
     gene            complement(7350..7682)
                     /gene="saoD"
                     /locus_tag="ACGI57_000696"
     CDS             complement(7350..7682)
                     /gene="saoD"
                     /locus_tag="ACGI57_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE-related protein SaoD"
                     /protein_id="HFG9715783.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            complement(7938..8774)
                     /gene="nadC"
                     /locus_tag="ACGI57_000697"
     CDS             complement(7938..8774)
                     /gene="nadC"
                     /locus_tag="ACGI57_000697"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFG9715784.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            complement(8755..10056)
                     /locus_tag="ACGI57_000698"
     CDS             complement(8755..10056)
                     /locus_tag="ACGI57_000698"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFG9715785.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            complement(10069..10983)
                     /gene="nadA"
                     /locus_tag="ACGI57_000699"
     CDS             complement(10069..10983)
                     /gene="nadA"
                     /locus_tag="ACGI57_000699"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFG9715786.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            11401..12858
                     /locus_tag="ACGI57_000700"
     CDS             11401..12858
                     /locus_tag="ACGI57_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFG9715787.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            13081..13509
                     /locus_tag="ACGI57_000701"
     CDS             13081..13509
                     /locus_tag="ACGI57_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715788.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            13569..13877
                     /locus_tag="ACGI57_000702"
     CDS             13569..13877
                     /locus_tag="ACGI57_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HFG9715789.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(14024..15046)
                     /locus_tag="ACGI57_000703"
     CDS             complement(14024..15046)
                     /locus_tag="ACGI57_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFG9715790.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(15221..15727)
                     /locus_tag="ACGI57_000704"
     CDS             complement(15221..15727)
                     /locus_tag="ACGI57_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFG9715791.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            complement(15787..16488)
                     /locus_tag="ACGI57_000705"
     CDS             complement(15787..16488)
                     /locus_tag="ACGI57_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9715792.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            complement(16706..17776)
                     /gene="buk"
                     /locus_tag="ACGI57_000706"
     CDS             complement(16706..17776)
                     /gene="buk"
                     /locus_tag="ACGI57_000706"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFG9715793.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            complement(17829..18404)
                     /locus_tag="ACGI57_000707"
     CDS             complement(17829..18404)
                     /locus_tag="ACGI57_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HFG9715794.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            complement(18406..20193)
                     /gene="iorA"
                     /locus_tag="ACGI57_000708"
     CDS             complement(18406..20193)
                     /gene="iorA"
                     /locus_tag="ACGI57_000708"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HFG9715795.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            complement(20254..21513)
                     /locus_tag="ACGI57_000709"
     CDS             complement(20254..21513)
                     /locus_tag="ACGI57_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9715796.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            complement(21775..23775)
                     /locus_tag="ACGI57_000710"
     CDS             complement(21775..23775)
                     /locus_tag="ACGI57_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9715797.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            complement(23783..25613)
                     /locus_tag="ACGI57_000711"
                     /pseudo
     CDS             complement(23783..25613)
                     /locus_tag="ACGI57_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(25697..27538)
                     /locus_tag="ACGI57_000712"
     CDS             complement(25697..27538)
                     /locus_tag="ACGI57_000712"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9715798.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            complement(27669..28088)
                     /locus_tag="ACGI57_000713"
     CDS             complement(27669..28088)
                     /locus_tag="ACGI57_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HFG9715799.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            28401..28583
                     /locus_tag="ACGI57_000714"
     CDS             28401..28583
                     /locus_tag="ACGI57_000714"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715800.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            28912..29934
                     /locus_tag="ACGI57_000715"
     CDS             28912..29934
                     /locus_tag="ACGI57_000715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFG9715801.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(30027..30629)
                     /locus_tag="ACGI57_000716"
     CDS             complement(30027..30629)
                     /locus_tag="ACGI57_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HFG9715802.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(30646..31794)
                     /locus_tag="ACGI57_000717"
     CDS             complement(30646..31794)
                     /locus_tag="ACGI57_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9715803.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            complement(31787..32164)
                     /locus_tag="ACGI57_000718"
     CDS             complement(31787..32164)
                     /locus_tag="ACGI57_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9715804.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            complement(32802..33281)
                     /locus_tag="ACGI57_000719"
     CDS             complement(32802..33281)
                     /locus_tag="ACGI57_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFG9715805.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT        11004 a         5680 c         3531 g        13073 t
ORIGIN      
        1 taatcatgtt gaataataac atatgattaa tggaattaca atattttata acacaaaact
       61 aactaatata acaaattacc atcagtctat ttattcaaac ttctaactat ataatataca
      121 tttatttata ttaacaatgc aaacgatacc attatttgta attaagaatt ttggaattat
      181 tgattacaaa gtttttacat tctcatctca cataagtaat atttttatca aaaaattaat
      241 attattaaac tttctaaatt acttatctct tgatataata gtatacaaaa caaaccatat
      301 ttcccactga aactttgttc aacactagct gtgtcaacgt tttcacagga aatttgagta
      361 tataatttat tggaggttga tatgtacaaa ttaatcgcac tagacattga tggaacaata
      421 ttaaatacac aaaaaaggat tactcctgaa gtctttgagt ccattcagga ggcaaaaagg
      481 gctggggcaa aagtagttat aacaacaggc agacctcttc ctggtgtaaa agaacttcta
      541 aatcaactaa atttaacaga tgaaggtgat tatgttatat gctttaatgg agcaattatt
      601 caagaagtta aaagtgagaa aatcatacat gatgtagaaa tgagtttaga tgattttgat
      661 tttatatata ataacgtttg taaaaaatat aagactaaaa tacacataaa tacaatgaca
      721 aatttaatta caccaaatga aacgcctggc aaatatacac ttcatgaagc gaaattaaat
      781 aatatagaag tcaaatatat acaaaaagat aaaattgatg aatctattaa aatctgtaaa
      841 attatgatag ttgatgaacc agaaagattg gaagaaatca tacagcaact acctaaaaat
      901 ttattcaata aatatactat agtaaggtct gctccttttt atctagaatt tttaggtaaa
      961 actactaata aaggaactgc tcttaaaaca ttatgtacga atctaaatat acctatagaa
     1021 aatgctattg ctgttggcga tgaagaaaat gaccaacata tgataaagta tgcaggtctt
     1081 ggtgtggcta tggggaatgc acgtaatagt ataaaggaaa ttgcagatta tgttactgat
     1141 actaataatg aaaatggagt tgcaaaggtt ataaataaat atatacttaa taaggctatc
     1201 taatcatcta aaaaaactaa aaatatacct ttagatttaa atacttttca catccctact
     1261 ctatactaat ttatatagta tggatattac ctatcatttt tatctaaagg tcatttttag
     1321 tttttcatat atgtatatct atgatagtac cttttgtttg agtttaaata ttttatatga
     1381 ttctactatt gcctcattaa tatctaaaca atatatatct tcatcatcag ataatcccgc
     1441 ttcgtttgtc caagcatcaa aatcactctt cgtccagaag aaattcatta tatttcagca
     1501 actagcagcc caatttagat gcttatcaag atttatatga agcactctta agtttggatt
     1561 attcacatac tcaatctttt cattttttat aattacttct atttccttac ctgtagcact
     1621 acaaatagag tttattttta tatcttggtt gaatgtgcaa gaggttccta tagcatctat
     1681 agcacacatt gcactgaaac ttctattatc tctcaaagtt actttatgat ttgttggcaa
     1741 tgctgataca ggatatataa aatttatact attcccatca acaaccatta tatttttata
     1801 aataaaatat tgtagtgtcc tttctatata ttctttttct atattcaact ttttacttac
     1861 atattcagtc acttcttgaa gtgtaacact agaaccttta tctattatca tatccataat
     1921 agagattctt attttctgtt gtgtattatc caaactattg tatttttcta tttgactcat
     1981 gtctactcct ctttaaactt cataacactt tgataaagta tcagttaatt tgtttttgta
     2041 ctcttcatat ttttggcttc ctctaattct aggtcttgga atatctatat ttatatcatc
     2101 aataattctt cctttatctc tcgacattac tataactcta tcactcatat atacagcttc
     2161 atcaacatca tgagttacca ttaatgcagt tattttcttc tttatccata ttcgttctag
     2221 ctcttcctgt aaattttgtc tcatttgaaa atctacagct cctaatggtt catccattag
     2281 taatacctct ggattacatg ctaaagctct tatcatggcc actctttgtt tcattcctcc
     2341 agataattga tgcggaaaca gtttttcttt tcctttcaag tctgacataa ccaaaagttc
     2401 ttctagcatt tcttctcttt tttcttttgg tattttctgc tgtttcatag gaaatagaac
     2461 attacctctt atagttttcc aaggaaataa agcataattt tgaaatataa aagctctatc
     2521 tatgcccggt tttttaactc ttttatcatt tataaaaaca tctccacgtt ctgatttttg
     2581 aaacccacct attatagtaa gtaaagttgt ttttccacaa cctgatggtc ctaaaagaca
     2641 gacaaattgt ccatcatcta tatccatact tatatcttct aaaacctttg tatgtactct
     2701 attatttaca aatgtcttat ttatatctct tacacttaac ttcattaatc tgcaaacctc
     2761 catctacaaa ggattttatt tataaacttt aataccctat ctattataaa tcctattaaa
     2821 gctgcaaata tcataagccc aattaataca tctgtatgca ttaatgtttg agcttgtacc
     2881 attgagtaac cgagaccggc actcgtagct ataaactcgg ctcagataac agacatccat
     2941 cctgtactta tagcaatcct tgaaccagtt agtatatctg gaagagatgc gggaacaatt
     3001 acatttgtaa atatcacaat tggcgatgct cccatacttc tagctgcatt ataataatcc
     3061 tttgatattg ctcttactcc ttgaatagta tttaaaatta tagggaaaac ccctgaaaaa
     3121 gctattaaaa atattgttgg accatctcct ataccaaacc atactatagt aagaggaacc
     3181 catgccatta ttggaacttg ccttacagaa tctactactg gagataaaac cctttcaaaa
     3241 gttttagaaa atcccataat aaatcctatt ggaagcccaa acaataatgc atacataaaa
     3301 cctttaagta ctctctgcaa tgttataaaa agattagtga ctgtctctgc atctgtcaca
     3361 cagaaaaaga aaccttttat tacatctact ggcattggaa gtaacaaaga actccctata
     3421 tcttttgctg caaaatgcca gactaaaaat ataataacta atagtattat tttatataaa
     3481 atatccatac taccatcagt ttttttctta gttaatctat cgcttttatc tttagaaatt
     3541 ttaatttcca attgcttatt tcctccatat ctatatttct tatattcctg tactctttta
     3601 atactttctt aaatgtagct ttattcttta aagcatcaac acttttattg taaagtgtta
     3661 taaaatcact atatgcttta ctttttttaa actctttatt tacaactaat acataggtat
     3721 cataatctcc taatttagtt gtagattttc ttttaccctc taacccaatt gactttatgc
     3781 aatctattac aacaccatct actaaattac tttctaatcc atatgctagt gcagtcccta
     3841 ataagggagc tacttccaca ttcttgtagt attttttaac caattctgac tggtaatctc
     3901 tattttgaat gactcctatc ttttcaggat tattatcact taatagaaat atatcagaat
     3961 tttgaactac agtaccaaca atttcaaatc cactatttac atttacatat tgttttgcag
     4021 catctttaca tatgattgct atatctattt cctctgaact cagagcccac tgcattgtac
     4081 ttgctcagca atcattaatt gtaaaaatat ccattatatc acttactttt atattttcaa
     4141 agtagtctct atttaccata taatttatta caaaaccaga ggtatcatca gataccccta
     4201 ttcttaattt tccttttatt tcttctttat cttcaaaatt aaaaaatatt ataaatatac
     4261 ttcccaaaat aatgataaaa agtattatta tatttttcat attctccatt tatttataat
     4321 acctttgctt tccagtcttc ataactcata ccttcaggaa actctgggtt aactttttct
     4381 tttataaatt tgtcaaaatc atcagctcca caagagtcca ataagcttgt atcaatcgta
     4441 tcttcatatt taggtatatc atctaaagcc tttaaatctt tatacatttg taaattattt
     4501 ttatattctt cacctgtaac tttccaagta agagttctac cttctccaac agtcttctta
     4561 tagatagttc taagtgcaac ttcttcttct acattataat attttgcaaa tattttagct
     4621 gcttcagctg gatttgtata tatatattct attgcttttg tatgtgctag aaccaatttt
     4681 tttgctaaat ctggatgttc ttttataaag tttttgttta atgagaatga acaacagtta
     4741 tattctttgc cattagcttt ttctttatat tgagtagatg ctattatttt tcctgcaccg
     4801 tcattttcag ctacagaacc ccaagggtca caagatgtaa aagcttgaat tttcccagtt
     4861 ttaagagcta aatatttatc tttgtctgaa tccatattta caacttcata ttttgatggg
     4921 tcagttggta gacctgtttc tggaccataa tcacttgtcc ataagaaatc actcatatct
     4981 gttgcaattt ttttacctat taaatcttta ggttctttta tatcttttga aactattaaa
     5041 tattcagaac caccagtatg gttgttagct gctattgtta ttggcgaacc cttaggtatt
     5101 gctccaacta accccttagt tcctatgtaa cctgcatcca tttttcctgc tgccatagct
     5161 tcaggaactt taccatttcc aacagttttt acattaagac ctaaatcttt atatattcct
     5221 gccgattctg ctactggtcc cgcagtcata tggtcacaat tataataacc tactgttatt
     5281 gtataatcat cttttccagt ttttgcttct tctttttctt ttggtttact actacatcct
     5341 gttaatatag atgtacccat tacaccaaat aagattaatg cttgaatctt cttagataat
     5401 ttcatataaa tttcccacct ttatgtatta tttttatttt tagcaacatt tgtccatatc
     5461 ttcgccaaat aaatcaacaa attttttacc ctctactctg taaatagcat atcctacatt
     5521 accattttta gaccctgata caattacttc tattggtgct ttatcatcaa tatttttaaa
     5581 ggttatcgtt tgattctcta taggtgctgg tattggcttt gttatatagt ggctattttt
     5641 atcagaaact acgactacca tttcatttga attattgctt tttttatata taactactaa
     5701 atcttttttt ccatcattat ttaagtcttc ttcagcacaa gttattatgt cttctttata
     5761 attttttcta aaatattcta atgtttcttt attcacattt ggaatagaag catcttcttt
     5821 gatgttatca tcaactattt ttgataaaat gattaacaca actactatac aggatattat
     5881 aactatcttt ttaagcatga tttcctatga cctctttcat ctttggtatt attgatttaa
     5941 atgcaaataa aataattaaa atagtacata catactcaaa actatcaggt atatttaaca
     6001 ttagtttatt tgccaactgt attgaagaat ttgttgcatc taaatttact gagttattat
     6061 tcactaaaag taaatttgtt atatatccaa ttattattga agacagtgtc aaagttacag
     6121 aatatataat aacagttctt tttccaataa gtttaaacat acttataagt tctggtagat
     6181 ttgtagctgc tccagctatt agaaatgtta tcgctatacc aggagatgct ccacttgcaa
     6241 caagggctgc tataaacggt atatgtccta cagcacatac atacataaca catgcaagta
     6301 ttgctatact tccaagagat agcaaacctg gatttcctaa atatttttga attatactat
     6361 ttggaaaaat agttgttata aatcctgcaa aaaccatacc cattactaca tatttactaa
     6421 tagtaagggc taattcacca aaagaccatt taagaccatc aatcatattt cccataaaac
     6481 ttcttttttc ttcttctaat tctattactt cttcctctga atcattatga agtgataatt
     6541 cttcttttcc aaaacaattt cctaaaaatc ctactaaaaa tggtactaaa aaaccagcta
     6601 ttagataaat gattgtaact ttaggaccta aaagacctag acaaagaact acagctattg
     6661 gatttattat tggagttgat gtcataaaag caagtactgg tcctaaatat gctcctgaat
     6721 aatacatact tattccaagt ggtattactc cacaactaca tattggtaaa aacattcctg
     6781 agatagtact tttaattaaa gaagaaaatt tcttatttcc aagttgtttt tgaaaccttc
     6841 ttggagttaa aacattgtga agtacacctg ccaatataaa acttatcata agccatcctg
     6901 atgctccatt cagtgtaact atagaagcta ctatagtatc tttaactcca tttaaaacaa
     6961 tttctaatat attcattttt gatactccta atttttaaac ttttaattat tgtttatagc
     7021 ttctactatt gccctttcaa ttgtatcttt atttaatcta tcatatttct ttttctgatt
     7081 tattattaaa gttccttttg ttataatccc atattttttt atatacgaaa aatctttccc
     7141 tgcttgatat aattttattt ccaatttatc agcatatttt tctgacattt tttttataaa
     7201 cactccatgt tcatcacaag atggtcaagt gtttataaat tcaactaaaa ttttagacat
     7261 ttgttaatcc cctactttaa acaatttatc ttttacttaa aataatccac agaaaataaa
     7321 tttttctatg gattgtatcc tatactaatt tataacgtta taacttggtc cacacctgca
     7381 ctgcctaaac aagcatatag gtttgggaaa catccaattg tagctccatc aaccaaatta
     7441 ttctctatac ctctttctat agcacattgg tcacaagcca ttattatcat atcagttttt
     7501 tcatgtaatt tacttagtct ctcgcctaca ggattacctt tagataaaaa taaggtatta
     7561 tccataaaga aaaacatccc tagaacgtct actccatgag ttccttgttc aagttgtggt
     7621 attatcattt tgtctagaat tttatgtgta ttagtactac taaaaatata agctactttc
     7681 atttttattc cccctatact ttttatattt tacaaaattt tacagataca attatattat
     7741 aagtttattc ttatttatct gtaaaataga tattaactat attattaaaa gtgtatatag
     7801 tatttatcta tttttattaa attattgatt gatttttttc tatttttatt aattttaatg
     7861 aataaaaaat agactcctta aatttataaa acaaatttaa ggagtctatt ttaattaagt
     7921 ttatttcaat ataaaattta tatatttctc aaattcttca tacttatatc gagagcttta
     7981 acagaatgtg taagcgctcc tacagaaaca acatccacac caatttttcc tatatcttct
     8041 atagtatcta aatttacatt tccggaaaat tctacaatgg ctcttccatt tataatatct
     8101 actgcttgtt ttgccatttc caaactcata ttatccaaca ttattatatc tgcattagct
     8161 tctattgctt cttttaccat gtctaaagtt tccacttcca cttctatctt tctaacaaaa
     8221 gaagtatttt ttctagctaa ttcaactgcc ttcttaacac taccagcagc cccaatatgg
     8281 ttgtctttaa gcaatattcc atcagataaa ttaaatctat gattgcatcc tccacctact
     8341 ttaacagaat acttatctaa tattctaaga ttaggtatcg tttttctact atctaataat
     8401 tttgtatttg taccttcaag cttttcaaca tacctacttg taatagtagc tataccactc
     8461 attctctgta aataattcaa tgctacacgc tctcctacaa gtaagtttct agtactacct
     8521 gttaatacag ctatcttttc acgattcttt actctatcac catcattttt atataatttt
     8581 acatctacat caccaagtat ctcaaaaact ctcttaaata cacctaagcc agctattata
     8641 ccatcttgtt tacaaattaa atctacagta gatttagaat tttcatccac aatagaatta
     8701 gttgttatat cttcacttgg aacatcttct atcaaagcat cctgtatcat tctatcaata
     8761 atcaaataat tcatcgccaa aatcctcact tgttctttca atttctttta tgactaatat
     8821 tttattttct ttttttacct gttctatatc tatatccata tctggtacat ctataaactt
     8881 aacttttaca ttatctataa ctgagtttat atttttagct gctctttttg aaaatacaag
     8941 cccttcaagt aatgaattac ttgccaatct attggctcca tgaacacctg tacaactaat
     9001 ttcaccaact gcatataaat tttccataga tgtttttgaa tccaaatcta catgtatccc
     9061 gcccataaaa taatgttgtg caggagatac ctttatacat tctttcgtta tgtctgttcc
     9121 tctagctaaa cattcattat aaataaaaga aaatctatta attaaatatt cactatctaa
     9181 atgtgttgca tctaaataca cataaggtaa tttatctttt tccatttgct caaaaatagc
     9241 ttttgaaact acatctctag gaagaagttc atccacaaat ctttctttat ttttatttaa
     9301 aagtttagct ccctctcctc ttaatgattc agatataagc attctcctac cttcattatt
     9361 ttcttcataa aaggctgtag gatgtatttg aatatattct aaattttcta tttttatatt
     9421 atttcttaaa gctattgcaa ttccatcacc tgtaagatgt ctttgattag tagaattttt
     9481 aaaaagccca ccaattccac ctgtagctaa aactactatt tttgaaaaaa catgaatctc
     9541 ttttttagat ttaaaaattc tagctcctat acatttattg ccatctttta atatatctaa
     9601 aagataggca tcttctataa gagttatatt ttctctagtt ttagcttctt ttaataaagt
     9661 tttaaaaaca acttctccag tattatcctt actatgaact attcgattta cactatgtgc
     9721 tccttctctt gtgtaatcta tctctccatt ttctttgtct aaaggcatac catattctac
     9781 tatctgccct acattttcta tagattcatc tgcaagtact tgaactgctt caactctatt
     9841 tttatactgc ccagccttca ttgtatcttc tacaaaggat tctatatcat caatatttct
     9901 agcagttgaa attccacctt gtgcaagata tgtattgttg ttttcaattg ttgatttcga
     9961 tactactagc acatttaaac ttttatctaa atttaatgca cagtataatc ctgaaactcc
    10021 acttccaact attaaaacat cttgttctaa attcataagt ctatccctct atccttctgc
    10081 taatttgtgc atgttaagca gtgaatttaa agccttttcc atgatttcat cttctacaat
    10141 aacttcattt tccatatttt taagtgtact atataaattt tctaatgttg tctttttcat
    10201 atctgtacat agtatttttc caccaggaat ataaaagttt ttattaggat tatttttctt
    10261 taattcatgt aatatacctt cttcagttgc tattataaag tctttgtttt tactattagt
    10321 agcatatttt attattcctg atgtactacc tacataatct cctaaatctc ttatttcttt
    10381 tttacattct ggatgtacta atacttctgc atttttatac ttatctttta actcaattat
    10441 ttcttcagct cttatattgt tatggtattt acaatatccg tcccaaaata taaagttttt
    10501 atctggaaat tgttctgcaa tatatcctcc taaattttta tctggaagga atataatttc
    10561 tttattatct atgttttcta gtattttaat tgcactagaa gatgtaacag aaacatcaca
    10621 atgtgcctta actttagctg tagagtttat ataacataca accaaagcat ttggatactg
    10681 ttgttttaat tttatcacgc cttcttcatc taccatatct gccatagcac acccagcgtt
    10741 acttactggc atcaatacag ttttttcagg tgataatatc ttagcacttt ctcccataaa
    10801 ccttactcca caaaatacaa ccacttcttc tttacaatct ctagcaatct cacttaaata
    10861 atatgagtca ccaactgcat ctgctaattc ttgtatttct ggtggttgat aaaaatgggc
    10921 aagtattatt gcattttttt ctttctttag ctctttgatt tgatatgtta aatctttatc
    10981 cataattgca ccgctttcta tatgtactag tgtatataca cctgtaaact cattatatca
    11041 cttgactatt ctttcttcaa gaattttaaa cctcttaata ttgttaagat atagacattt
    11101 atattctcaa ttgattttcc ttttttctcc atctatttaa actaattttt atatttttta
    11161 atttttttgt aaaatattat cagaattttg tctatattct ttattgctat aacattgaaa
    11221 atattaccaa atacctgtat actaagtaca tggttatgta tatgtattta tttctgaaat
    11281 tatattagtt gtaatacttt atagaatttg taggttgtcc agaattaaat tcaatataaa
    11341 tctacacatt tagataatat gtatacaaag accaaaaaat tttataaatg gggtgatatt
    11401 atgacaacat tcttaatcgg cttggctata ttactaatag gaggtgcttt atacggtgct
    11461 tattgtgaaa aagtatttgg cccagatgac agaaaaacac ctgcacttgc tcaaagtgat
    11521 ggagtagact atgtcccaat gaaaaaatgg aaaaatagtc taattgagtt gcttaatata
    11581 gctggtacag gaccaattct aggacctatc caaggtatat tgtttggtcc aatagcattc
    11641 atactaatac ctattggctg tgtatttgga ggagctttgc atgactatat gagtggaatg
    11701 attagtataa gagaaaaagg agcacaaatg ccttctctta tcagtagatt tttaggtaat
    11761 aaagtatttc aagtctataa tatattcctt tgtcttttaa tgcttttagt tggtgcagtt
    11821 tttatttata cacctgggga tttagttgtt actcaaattt taaatatgaa atcgactata
    11881 aataatccag ttgtctggat agtttatgga cttatctttc tatattactt gtgtgcaaca
    11941 ctatttccaa ttgataaaat cataggaaag gtatatccaa tatttggagc tattctactt
    12001 ttatctgctg ctggtgttgg aattggaatc tttacacaag gttatgattt agctaattta
    12061 agtcttgcaa attggaaagg tattcatcct gatggtattc ctcttattcc tacattcttt
    12121 gttacagttg cttgtggtat tgtttctgga ttccactcaa ctcaagctac tcttatagca
    12181 agatcagttt caaatgaaaa agaaggaaaa actacttttt ataatatgat gattttagaa
    12241 ggtttaattg caatgatttg ggctgcagct gctatgggta tttacaataa aggaatacct
    12301 aaggaattag taggttctcc tgatgtaata ggtttggttg caagagattt acttggttct
    12361 attggtggta tcatagctat aataggtgta attgttttac caataacttc tggagataca
    12421 gctttaagat ctttaagatt aatgcttgca gattactttc attatgacca aaaggaaaaa
    12481 aaacatcgtg ttatattgtc aatctgtata tttattcctg tcatagccat ccttatattt
    12541 gcaaagctta gtgcttctgg atttaatata ttgtggagat acttctcttg gagtaatcaa
    12601 actattgcca tatttgcatt tgcaatgatt acagtttacc ttataataaa agaaaagaat
    12661 tatattataa gcttaattcc tggtatgttt tattcctttg ttatattcag ttatatattt
    12721 aatgctcaaa ttggatttaa cttaaacata aatatatcat atgttttagc tgccatattt
    12781 actgtattat atgctatttt aactgtacgc tctggtagaa agttaaaaag caaagcagat
    12841 actaagttgg ctgactaaat ctaaataaaa taatacttag aataaatttt attaaaaaat
    12901 gataaaaaag taggcaatct caagttagat ttgcctcttt tttatttaat acagtaataa
    12961 atacttaagt atttactcct tattggaata tttatcctaa atctctaata taaataaata
    13021 aaagttttaa aataatctat tcgtttatat acttaaaata aattttttgg aggaatactt
    13081 atgaataata aattacatga acttgaaaaa aatctaccag aaacatcttg ttctttttgc
    13141 acacatcttt catttaaggg gcctagccta gactatagat atgatattag atgtgttatt
    13201 tcagatacaa agcccgattt tagaggatgt tgtgaatatt ttgaaccaga atatacagaa
    13261 ttaaatacag gagatttaga caatctatac ataaactttc ttgagacatg cctcaaggta
    13321 aattataatg attacatcaa ttctatgtat tggaagttat ttaaagaaag aaccctacta
    13381 gaaaacaatt ttaaatgttc tatatgtgga tcaactgaaa atgttgatgt gtatcatgtt
    13441 aataaaaatt taggaaggga aacttccaaa gatgtagttg ttaaatgtga taaatgtaat
    13501 tgtaaataag tgtataaaat ttagataact tcacataata agtttaaata aaacttagga
    13561 ggttatttat gaaaaataat aatttaaatt gtgctgctac taattgtgct tataatacta
    13621 gtggatattg ttatgctggt agtataaaag tagatggaat gcaagcaact actacagaaa
    13681 atacttattg tgcttctttt gaagacaaat atacatctgg tataactagt cgttctaatg
    13741 aaactaacca agttgataca gataacattc attgtgaagc tgtaaaatgt aaatacaata
    13801 aaaatgaact ttgcaaagct gaaaaagttc atataaatga aagaaatgca agttgtgaaa
    13861 cttttgaaat gaaataaatt tctttttatt tagttttata gtatttatac aaagcaaaat
    13921 tgaacctatc aaatagatgt ctttattaat gatgtctatt tataggttct tttttaaaac
    13981 aatttataat ttatatataa agataaatta ttattttaat atattaccta gaagtaaatt
    14041 taacacccag aatatccata aggaacataa atattggaac tccaattagt aatccccata
    14101 cacctaaata atgttctcct acaagtaaaa taataaaaac aaaacaaact ggaagtttag
    14161 ttctattcga catcaactta gggtttaata tgtatgtttc tactgcatgc actatacaaa
    14221 tcataagtaa aactccaaaa acctttgtta ttcctcctgt attaaatgct attacagtta
    14281 atggaatcaa agacactata actcctgcta ctggaataag acccaacaca aaaatcataa
    14341 atcctaaagc ccatatttga gggaatccca tgaaacctaa gaaaatcata gaaacaacag
    14401 aatttataaa ggcaatcata acttgtactt tcattacttc accaaaattt ttaacaaagc
    14461 tctttccaaa aaatacaagc gaattataaa taaaagaaat cttactatca gaaagtttat
    14521 ctccaaaatt tttaatttca tttttttcaa gtaaaaggag taaactcagt aaaaatgcca
    14581 ataatatatt tactccaaaa cttccaactt ttgttgctgt tgatgctata agtagccctg
    14641 cttgtgaaat atatttatta aaatccagct ttgagacaat aggatacaat tttggtccta
    14701 aagattgagc aaattgattt acatcaaaat cactaaaaac cctactcaac tctgtaagtt
    14761 gaactataat tattggtaca actgcatagc taatcaatac taacaatata gcaaatagtg
    14821 catataacac tataattatt aatgaatctg gaatatttaa agaagaaaac ttcttaattc
    14881 gttttcttgt ggctacaagc aagttataga aaataaaagt cataataaat gttaaaagca
    14941 ccatatttaa catataccaa aatgtaatta gaataattac tacgacagat atagaaacaa
    15001 ttttatattt tgaactgtca cgtgccaatc ctttgttatc ttccatagtt taaatcctcc
    15061 ccaaattttt atgactttat aatgactgcc ctttttattt taatctcaaa aaacaagtag
    15121 aaaaatacta aaaataactt attaattttc ataaaacaat attaaaattt agagaagata
    15181 cattttaaat aatcttactt atactgaaat ctgctctaca tcatatattt aatttattat
    15241 tatctatctc atatagcaat tcgtatatct ctggatattc aaaaaacaaa ccctctttta
    15301 gtaattgtct tatttcatct gtgcttaccc actttatatc acttacttct tcctcctgta
    15361 aaatagcttt ggatatatca tattcttttt taactaagta tacatcccaa agagtatctt
    15421 catgaatcat acttctaaaa actttcatct catctttagt tatatctatc cctatttctt
    15481 cctttgcttc gcgaattgct ccttctaaac tgtcttcccc agaaacaata catccagttg
    15541 tcattcccca catatttggc aatgtctttt tacttttaga ccttttctga attaaaattt
    15601 gagagttact atttaaaatc cacacttcaa ctgccaaatg gtaataacct tcttcaatag
    15661 aattacctct ttttataaca tttcctgttt taattccatc agcattatat aaatcccata
    15721 attccatttc aaaatctcct tgactttact ataaattaga taaactacaa atatatttta
    15781 gcataattaa ttctgctcat tttttattac aatacttgat ttacatttat gttcattgct
    15841 tttcttttcc ttccattctc ttatatcatc tataataaat ctatgtttca acaattttgt
    15901 gtcaaattta aatattctaa acactatttg taatacatat cctaaaccta aagcacttac
    15961 aagcgtacca atacctacag aacctcctaa taaccaccca acaactaatg ctagtatttc
    16021 aagaattgtt ctaacaagac gtacagattt attagttttt ttctgaagag caatcataag
    16081 cccatctctc ggtccacttc ctaaacaaac acctaagtat agaaaacttg ctatagctgc
    16141 caaaataatt cctataacaa ccataaacac acctatataa gtgttgcttg catgaggtat
    16201 cactccagaa aatattacta aatcaataaa aattcctata aatgtcatgt tgagtaaagt
    16261 accccatcct atattttcac caaaaactgc atccaatata acaattacaa ctcctaccat
    16321 tatagaagcc tgacctatag ttatgcctag ctttatagat aaaccttggt gaagtacatc
    16381 ccaaggcata agacctacat gtgcattaat cataagcaca gaagaaattg caaatataaa
    16441 aaaacctgaa aataatctta tcatactttt taaacttatt tttcccatca aaaatcactc
    16501 ctcataataa taataacaat aaataccaat aaaatatata tataaatacg aaattttaca
    16561 gaattattta ttaggttaag ataaaatttt aattataaaa agtggcaatc caaattttat
    16621 acattttcac ctatactcta agctttcgtg tataaaaaac ggatatacca cttttttagt
    16681 atataaacat ttttagtttt tcttattaat tttcactata aattctagct tcttcttctc
    16741 catttagtac tcttaaagtt ccaaaagcaa gtgattccaa ctcattttct cctgccataa
    16801 tttctactgg tgctataaac tcaactttct ttttaaccat tgaagtaaac atttcagaat
    16861 atgcaattcc acctgttata attatagcat ctacctttcc atctactaca gttgctaact
    16921 ctccaatacc ttttgctaac tgataagcca ttgcttcata aataattttt gcttcttcat
    16981 ttccttctgc tatcattttt tcaacttctc tagcatctac agtatttagg tatgaaacta
    17041 taccaccttt acctcttatc tttttagtca tttctctttc tgaatattga cctgaataac
    17101 aggcagccac taattttgta gcaggtactc ttcctgacct ttctggagaa aatggtcctt
    17161 catcatcaga tagcatatca accattcttc ctttttcatg aagataaata cttactcctc
    17221 caccaatgtg agctactatt aagtttaagc tcttataatc ttttccttta tccttagcat
    17281 attttaaagc cattgctcta gtatttaatg catgccctgc acttgatcta tccattccac
    17341 aaagtccaga tattctcgct acatctataa gctcatctac tgcaacagaa tcataaatat
    17401 atgagttgat tccaagaggt tctgatattg catgtgccac aacagcacct aaattggaag
    17461 cgtgttcaag tactggtcta tgtcttagta catcaatcat ttcttcattt actaaatatg
    17521 ctcctgattt tactggtggt aaaactccac ctcttcctac tattgcacta agagatttta
    17581 agtctatact attttcttta agaatattca aaactgcttc cttacgcatt tcaaactggt
    17641 cttgtatagt attatatttt gcaatctctt cagctggatg gtctatcgtc ttcacaagaa
    17701 tttgttcttc tccatcatat actgctattt ttgtagaagt agaacctgga tttatggcta
    17761 atattctgta agtcataatt ttcttccttt caccttaatt tctatagtct atttatatct
    17821 attataaact atttgctaac catttccata ccttcatgga aagcttttat gtttaattca
    17881 acaaattttg gtttaacatt ttcacttata attttttccc aatcaataga ctctaattgc
    17941 atagatttaa ctagacatcc taataaaata acattcatta tttttgaatt tcctagttct
    18001 tcagctttat cagctgcatt tataactttt gcatttaatc tacttaactc tgcatctata
    18061 tcactttctt gatattcagc tcctccgatt aaaacagtca tagaatctat cctatgatta
    18121 ttcactaaag cttttccttc tggttttaaa taattgaacc atcttaaagc ttccattttt
    18181 tcaaaagata ctaatatatc tgctccacca atttctataa ctggagaatg aacacaatct
    18241 ccatatctaa cttgtgagga aactgaacct cctctttgac tcattccatg tatttcactc
    18301 atctttacat catatccagc ttccatcaat cccatagtta ataatttact agcaagtata
    18361 gttccttgac ctcctacacc taccaaaagt atactcttag tcatattatt tttcctcctt
    18421 aactattgct tgttttggac aaacttgtgc acatactcca catcctacac actgatttct
    18481 atctatattt gataacttat tctctttatc aaatgataga gctggacatc cagttttcaa
    18541 acataattta catcctatac atttatcatg gtcaacttta cacttagtct taaatggatt
    18601 attaaattct tctatatctt ctttagagaa ttttttaagt acacatggcc atcttgttat
    18661 tattactgat ggttcatctt ctatagctaa agcccaatct agtgcttcat ttacttcttt
    18721 taaattatta gggtttataa ctcttacatg ttctattcca caagctttaa ctaatccttc
    18781 tatatcaact tctttagttt tagcaccttg taaagtatat cctgaacctg ggttttcttg
    18841 gtgacctgtc attccagtta ttctattatc taatataacc gaaatagaat tacctctatt
    18901 gtaaacaaca tctaaaagtc catttattcc tgtatggaag aatgttgagt ctccaagtac
    18961 acctaccaat cttttttcat tatcatcctt catattaaga actttttgag ctccatgtgc
    19021 agttccaaaa gcagaaccca tacaaacaac ataatctatt ccattatatg gaggagcaaa
    19081 tccaagagta taacaaccta tatctccacc aactattaaa ttttttctct ttccaagttc
    19141 atagaagaat cctctatgtg gacatcctgc acagaaactt ggtggtcttg gtgttactaa
    19201 ttcagatttg tattctatag tatcatttgt ttttccaaat atagcttttc ttaaaacatc
    19261 tggtgtcatt tctccatatg gtggtattac atcttttcca atacaatcta ttccatatgc
    19321 ttttatttgt tcttctataa atgggtcatt ttcttctata acatatattt tatcaacttt
    19381 ttctgcaaat tcttttattt tttcataagg taatggattt gtaaatccta gtttcatata
    19441 tgatgcattt tttccaaaaa cttctttggc aaaattacaa cacataccag atgctataac
    19501 acctattttt gtatcattta tttcatagta gtttaaatca gtttcatttg agaatttttt
    19561 aagaatttcc attctttctt caacaacgcc ccttcttatt tgagcatttg ctggaacagt
    19621 taccattttc ttagcatttt taacatactc tttaatttca acttcttctc tatcatagca
    19681 ctccactaat ccttttgaat gacaaagtct tgttgtaact ctatataaaa ctggtgtatc
    19741 gtatttttca ctaacttcaa aagcttcttt tatcatatct ttagcttctt gactagttga
    19801 tggttcaaat aaaggtatct ttgcaaattt tgcatacatc ctattgtctt gctcattttg
    19861 agatgaatgc attcccggct catctgctgt aataagtacc ataccaccat ttacccctgt
    19921 atatgcataa gtaaatattg gatcagctgc aacattcaaa ccaacatgtt tcatagaagc
    19981 cattgttctt gctcctgcaa ttgaaccccc tatagcagcc tcaagtgcta ccttttcgtt
    20041 tggagcccac tctgcaacta ttgcatcttt atatgttgct atattttcta atatttctgt
    20101 acttggagtc cctggatatg cagatgcata tttaacacca gcctcatagg ctccacgtgc
    20161 aattgcttca ttacctgtca tcaattgttt catataaaat tccctccatc tacaattatt
    20221 aaattcatag tccacccaat ttgttcaatg aaattaatta tttattttta cttctaaact
    20281 ttcaagtgct tcttttataa tacttggtgc tttcttacat ttatcttcag aaactgcaca
    20341 aactgcaaat cttaagccca tttttaatgg aactgcaaat aaattatgtt tagtcaattc
    20401 ttcacatact tcttttggat tatcacatgg tatacttaca aagaaacctg ctatatatgg
    20461 aagaatttct aatcctactc tttcagcttc ttttacaaat acatctgctc ttctagtaag
    20521 catgtttttg tatatttctt tttcatcctc atattctttg aattttttag ggtcatttac
    20581 aatgttagat aatactgcca tagcacttct attacaattt gaccaatttg ctctacatga
    20641 atgaacacat gagtaatgga actcttcagc tacatcttca cttgaagata tacaaattat
    20701 agctcccatt ctcataccat aagctgtaaa tccttttgac atactgaatc caaccaatac
    20761 gagaacgttt tcaggcaaat tagaaaattt cttaaaaaac tttctacata catcatcatc
    20821 acctgcaaag tcaatatatg ctgaatctac gaaaaatatt atctttttgt ctttatcttt
    20881 tgcgacttct tttgataagt ctaatatttt atcccactca tcatctgcaa cactatatcc
    20941 agttggatta tgcgctggtg tgtttataat tgtaaagact ctaccttgtt tttctgctat
    21001 atttacaaat ttttctttaa acgactcaaa attaaacctt ctattttcat caaaaagttg
    21061 gtaattaact acttttctat tagcctcttc agctatactt acatatggac tccagaacca
    21121 atcagatgtt aatacctcat ctccttcatt agtatagttc catacagcta attttattgc
    21181 accacttcct cctggtgaag ctaatactct tatatgtcct tctggtaagt aatctctgaa
    21241 aaatactttt ttaactgctt ctaagtaatc tggttgacct tcaagagctg catatgcacc
    21301 tatctcacag ttatctaatg ctttatattc ttcatataca gttttcattg ttatcaattt
    21361 tcctgaatca tccattagag cgcctattgt tgcatttatt acattatcca taccaaaagc
    21421 cttctctgct gcttgagctc tgccagatat gctaaatata acatcatttt cttttggcca
    21481 cattgcatgc ttaccaacca tactttgact cataccatca cccctaaaat tgtatttact
    21541 cttacattat atattaagca attactatgc caataaatcc cactttatgt aaaattgatt
    21601 ttgtttagaa ttattatttt tttcattatt aaattaagtg ttttaactat ttttcttata
    21661 aatatatatg cctgtctaaa aaaataaaaa tttgcaaaaa agtaatgtta atttttctta
    21721 acacttgttt taaaaaatta acattacttc aaaataattt atatgtaatt atatttaaat
    21781 atttttattg tatttcttcc ataaagatgt cctgcttatt ccaagaactt gtgcaacttc
    21841 atttttagaa agtccctgag caaaaagcat acttattatt ttttcttcaa catacttatt
    21901 tacttctttt atatctatac ttatcttacc atcatttatc ttacttatat ttatagattc
    21961 atcttggact tctacacttg ctgtcgtttc agaattaaat ttgtctactt cagagttagg
    22021 aagtaatttt ttagttttaa aatctctatc ttgtctcaat ttaattttac ctgtcacaac
    22081 atatttttga gctattgtct ttaactctct tacatttcca ggccagctat aattatatag
    22141 cttatccatt tcatctttag tcaaaacaaa attttcatct atactgttta ttccattgag
    22201 accatcatcc tttaatactt catttacaaa attgttaaac aaaggtatta tatcttctct
    22261 tctgtctctt aaaggaggta tatttatctc taaactactc aatctataaa ataagtctgc
    22321 tctaaatgta cccataacta ttttttcggt taaagattca ttcgttgctg ctataatcct
    22381 tatatccaga ggtattatat aatcagaacc aattctcatt atttgacgtt cttctattac
    22441 tcttaatagt tttgtttgaa tattaaagga tagactattt atttcatcta aaaaaagagt
    22501 ccctccatga gcaagttcaa atagtcctct ttttccaccc tttctagcac cagtaaatgc
    22561 accttcttca tagccaaaaa gttcactttc aagtaaattt tctgctatag tagcacaatt
    22621 tatagcaaca aaaggcctat cttttctttt acttatgttg tgtatactat gagctataat
    22681 ttctttacca gacccacttt ctccataaag taaagttgta taatcacttt ttccaatttt
    22741 tttagcttct tcaataaatt ctttagttaa tttatcttta aataaaaaat catcaaaagt
    22801 atatctagca tagagacctt tttgattcaa gtcaaatctt atttttcttt ctaagttttg
    22861 tagcttggtt atatcttgta ttattcctaa aactccataa gtactattat caacttttat
    22921 caatgttgtc ctagtattaa caattagatt atttatattt cttatctttc tatcttctac
    22981 gtcctctttt tcatgtaaac aatctaacat ccactccatt ttaggaaata catctaagat
    23041 gtatttatta agtgcacatt ttctctctac tttaagcatg ttttttgcac tttcattgta
    23101 caaaattata ctgccattac tatctatagc aattactcca tcttttatac catcaagtat
    23161 atttcgtaac acttcattat taaacttttc ttcacctaat gtatctaata attttttaca
    23221 atattctaca gcttctctta tagattcatc actagcagtt gcaaatacac tatcaattcc
    23281 atattgcctt gcaaaagaac atgccaaacc tcctcctacg attacaactt catctttttg
    23341 gtctatatat tttacaactt tacttcttat ctcatactta gattcaaacc attcttcagt
    23401 tatttcagta cttatgacat ttctccagcc aacatagtca aaagaaactt cattgtctcc
    23461 aagaatcaat actacttttt tagaatattt ctttgctatt tctattgctc ttaaaaggtc
    23521 agttgacttc atattcattg gtataacagg tacatttact gtatctatta ctaagctata
    23581 acctccacct ctaccaatta tagcttgggc acctttttca acaagtatac gaccttgatt
    23641 ttccattaag tctaggttaa gtatgtcgat tattatagtt ccattttcta catcttctct
    23701 atataattct tctattcttt cttttaattc aatatcagag gctataattc ctattttttt
    23761 tcgcaacaaa atcacctcaa ttttaaatta taatgtattg atttctacct gctttttttg
    23821 atttatatag gttcttatct gcatcttcaa ttaatatttc aacatctttt ctactacgac
    23881 acctttttat acttactcca gcacttatag ttatatagtt ttttatttta gaatatatat
    23941 gttcaatacc ctcaaattca agaagtttga aaactctttt aatatataat tctatattat
    24001 caatctcttc aacaccgatt gattctatag aatctactaa tattaaaatt tcttctcccc
    24061 catatctgat tatgtgttcg gtaatatcta taaatacact tcttataata tcagttacct
    24121 taactataac tttatcccct gctagatgac cataattatc attataactt ttaaaacaat
    24181 ctatatcgac cagtattgcg accatatttt tgtctctggt tccattaaat aaatccaact
    24241 gctgttccaa atatcttcta ttatatagtt tagtaagtcc atcttttaaa atatcatttt
    24301 ctaatttctt attatcatcc actagtaatc ttaaactctc ttgtttttgt ctgagttcat
    24361 attgtttgtg aattgcttga gatgttttat aggaattatt tttttcccac ctatcagaat
    24421 accaatccat acttttataa tattctattg ccagtttaaa atcttttttt agctcatatc
    24481 catttgctat tagcccatat aaatatagat atttttctaa gattcaatga actcttttta
    24541 actaaattta aaccttttat ataacaactt ataccttcat ctatattacc aaattttata
    24601 tataaatctc catacaactt atatatgtaa tctagtttag aattatatat aaaaacatct
    24661 ccaaaacttt tatatatatt ttttgataaa attaaatagg tgtttacctt ttcaaaacta
    24721 caattatctt tctttaactc aatttcagct gaatataaaa atataaatga atataaataa
    24781 tccttatata caccttcaac aactttcatt aattttatag tctttaataa aataacttct
    24841 gcttctttat tcatatcttc ttttaaatat gcccatataa ggtctatttt tatagacact
    24901 ctttcagtta ctgatattga gttattactg tgtgcaacat ctaatacatt atttatgtat
    24961 tttatagctt ctacattatt tccactctct aaataacaca aacttattat acatttacaa
    25021 aaagcctcta tagagtataa attattttct tgagccaatt ttagacattc aattaagtat
    25081 tccatacttt ttataaagtt attaaataaa tatagataaa ctctcccaaa tatataattt
    25141 gccctactta caagagcttt attttttaaa gttttaaaaa aagacacttc ttctactaaa
    25201 gctattacat ccgttttggt ctgatataaa gcacttattt ccacacataa actcatattt
    25261 aacaatacca acaactcatt taaatgattt tgccttataa ttttttttgc tgagaaataa
    25321 aaattattta ccttatcata ttttcttata gaaatagctg ataaagtaag ataaatataa
    25381 attttagata ttatatctga atcatctttt aatgacaatt ttttaatata ctttaatgac
    25441 tctatcaaat agttgtatga tgcttctcta tcttttttta tatagtttat aagactaatt
    25501 aaatataaag tatagactct actattatct tctaattttg tatttaaaag gtctttgtga
    25561 taatccaaga tgtagtttaa atctgtcaaa tttagataga atttagtcaa catatcgtat
    25621 tctatcttgt ttatatttct ctttaaatct tctattaaac tccttttcaa gtaaatcacc
    25681 aatcctattt caacagttaa aaattcttaa aatcatgata atgcaaacat ctatttctac
    25741 cactttcctt tgctttatat aaagctttat cagcattttc aattatttct tttatatcta
    25801 tactactatt tttacatgta tcaatgccaa tactaactgt caaattatta tttaaaaatt
    25861 tctttacaat cctacataat tttcttccaa atattttact ttttttataa tctttacata
    25921 cagataatat cagaaattct tctccaccat accttatcat aagcatgtct ttttttagat
    25981 atttttttat tattatagtt atatttttaa gagcaatatc tccattataa tgtccataag
    26041 tatcattgta ttctttaaag taatctacat ctatcataaa tgctgtgcaa tattcactaa
    26101 cttcttgttt ttctaaaaaa tcgttcaaaa aagttctatt atatgcactt gtcaaatggt
    26161 caacatactt ttttttatct aaatcaagtt ttacttttct catgttattt atttcttttt
    26221 ctatatactc atgtctataa tttaaggaat ctatataatt tctatttcga gcttttatat
    26281 acatcatttt taactcataa tacattttaa agtatttcat actcatttca taattttgaa
    26341 tcttttcaaa acacaaatat atactcttgt atgctaactt cacataatta taatttttag
    26401 catttttaga tttttcaagg agttttttat ttgtatttaa ggcattgtca taatatccaa
    26461 agatataata tatctttgcc tggtgataaa ttatttctaa atcatatatt atatattctt
    26521 ctatattttt tatcatattt aatatgtttt tagctctatc taaatagcat attgattgtt
    26581 caaaagattt ttcattactt tttgcataat acatggctaa acttataaat aaatcaaact
    26641 tctctacttc ttttaagcta tgatttttaa ttttttctag atatacttca gattttgcta
    26701 aattatttat tgcgacagga taatttctag tctttattaa tattttagtt atgttgtttg
    26761 atgctctata tttttgaatt atatttacat ttctatactt tttattgctt attacttcat
    26821 tgaaatattc catggaacat tcataatttt caaacgaaca ttcacactca cctatcttta
    26881 tattacatag tgaagatact tgcatatcct ttagtaaatc tgcatatttt cttgctttta
    26941 aaaaatttac cttagcaatt agagcatcat ccaaaaacaa aaaatatatt ttaccaataa
    27001 taaaaaaata tattttttta caattaaaat cgttaatttc ttttatcttt atatacaacg
    27061 tttctatttc tttaattatt acagaagatt ctatacactt acttgcctta atgtaaataa
    27121 gttgtgattt aaaaataatt tcttcaatta gaaaattatt ttcatcacaa ataagattaa
    27181 atttatccat atataaattt gccttattta catcttcatt ttttaaactt aaaatagcta
    27241 tttgtgaata aaatcgtgct aaaaatttat aatctttggg taagcattta tcaatatatt
    27301 tacaggaaat caaaatatac tctttcattt tttcactaaa cccttgaata ccatataata
    27361 aagctagcaa aaaatttgat tttaataaca ttaaactatt ttgttcagtt ttatagcaat
    27421 caattaaatt ctttactaca tcaattttat tttcaataaa atcgttatta gtcaataatc
    27481 tttcattcaa ggttagttta aacattgaat ctaaaattgt atcatctatc tcttccaaat
    27541 atttactata tattgtaact cacctccgac ttacattatc tcataaattt agtaattttc
    27601 catactattt ttgaaaaaag ttgatgtctc aatttagaaa tttctcttaa attaagacat
    27661 actttacact aaactataca attttctatc tgaactatat aatcctgaca attttcgcaa
    27721 attatcacac ttacaatata actattatca actttatttg cccttgatag tttattaaaa
    27781 gatagtcctt tataatcaac agatatattg cttattacat attctccatc tttactgaaa
    27841 atataaaagt gatattctaa aaaaggaaag aattttttct tttcttttac tccttctaaa
    27901 cataatggac atgcttcttg ataatattct acatcaaaat caacttcttc tgcttcatta
    27961 agtatatcaa ttatttttga aatacttact ccttgaactg gattttcttc atcatttatt
    28021 aaagcactac agttctcctc ttttaataaa tattccttat catcatatat aaacttatat
    28081 aaactcatat tatatcctcc ttataattat taatctccaa gttattctac catttaaata
    28141 aagttaataa aagtaattgt ttataatctt tataattaag ttatgtatac atatattcct
    28201 atgcgctatc ttaattaatg attttaaaat ccaagtttgc actttgaaca tccagtttcc
    28261 caaaatctat tgtcaaaact cactttagtt attaatataa tgatataaat atataactgc
    28321 atagatgtta cacacttaac tatttaatat gatacgattt ggtatctaaa tttatatatt
    28381 gtatataggg ggagatatat atgtataaag ataaaagtga tgaatgtata cacttaatga
    28441 ctgcatacat cgatagtata agtggatatt attcatttat tgacacacaa ttagaggatt
    28501 ttatgatgaa atatggtgaa aacattgttg attcaaattt acacagtata atgatgttgc
    28561 tttgtaaatg gggattatct tagcatttca agaaactttt ttgtatattg tattctatat
    28621 tatttattca ttaacaaaaa agattgacat aaacttaaaa gctatatata gatttcttta
    28681 aaaatctata tatagctttt ttaatttttt caaaatgtat acttaaataa gttagttttt
    28741 ttcaaaattt gactatctaa tatgatataa tttaactatc tacaacaata aaaaatatct
    28801 aatatgtatt atctagttta gctcatcata ataacatccc tatatttaca attaagatat
    28861 tttcaatata gtaatactca attttaatat tataaaaagg agagattttg tatggattac
    28921 aatgacttac taaaatcagc tagagagaac tttaatggaa catgtaaagt atgtaaaatt
    28981 tgtaatggat tagcttgtgc tggagatgtt cctggtatgg gtggtaaagg aagtggttcg
    29041 tctttcattg aaaatagaaa aagtctagaa aaaataaaga taaatatgag agtcatacat
    29101 aatgtttcaa agccagatac atccatagaa ttatttggta gaaaaatgag ttctcctata
    29161 ttcgctgcac ctgtatcagg tactatattg aacatgggtg gtaaagtttc cgaaaaagaa
    29221 tacatagagc ctgttgttag aggttgttct aattctggaa tttatgctat ggttggcgat
    29281 actaatgtag atactttttt actggataat ttagatgtat tgaaggataa taggggaaat
    29341 ggaattgtat ttataaagcc ttggaataat tcaaagataa tagataaaat tagattgtct
    29401 gaagaagcag gtgcttttgc agttggtgta gaccttgatg cttgtggact tattaataat
    29461 caatttcaag aaaatccttt ttctcctaaa acaattgatg agattagaga attggttgaa
    29521 tccactagat taccttttat tataaaagga attatgactg tagatgatgc tttgatggct
    29581 gtagagtctg gtgctagtgc cattattgtt tcaaatcatg gtggaagagt acttgattat
    29641 actcctggta cttgtgaggt acttccagat atagctaagt ctgtaaaggg aaagataaca
    29701 atacttgttg atggtggagt taggactggt gttgatgtag ttaagatgct tggattgggt
    29761 gctgatgctg ttttgatggg aagacctttt gttactgctt cttttggcgg tggtcttgat
    29821 ggtgtggagt tttttataga gaaggttagg aatgagttat gtgagactat gattttgact
    29881 ggttgtcaaa atgttaagga tatagatggt agagttatat ggaatttatt gtaaaatagt
    29941 tttataacat ttatatttaa atagtgtctc atttaaattg aaaaatcata ttgagacact
    30001 atttaatata tttttagatt ttaatcctat gaaagagtag ggtcagttag tctacccaat
    30061 ttatcccaag tagctggacc aattattcca tctgcagaca aaccgtattt tttttggaaa
    30121 cttacaactg cattatatgt cttacttcca aaagaactat ctatacctac attataacca
    30181 agtaaagtta acatgatttg tgcagctttt acttcttttc cgctattacc ctttcgtata
    30241 acttttgttg tacttatagt caaatactca ttactcataa ttgttatagt tttagcacct
    30301 atattataag aaacagtata acttgcataa acacctttac tattgtcctc attcattaga
    30361 ttagctgatt ctggaacaaa tatctcatca tctggcttag ggtctatatt tatctcttca
    30421 caattttgtt tatgttcatc tcttatttct tgtggcgcat tttcatatgt atatatatac
    30481 ccagaaagag taaaagaatc taggtttttc tctggaacag tctctttact tcctcctttc
    30541 tctttacttg tgtcagcaaa tgccacactt gtaaaagtaa gcaataagca acacataata
    30601 gctactattt ttcttaattt tgttaacata aaatcctcct tataattatt ttattaattg
    30661 attataatct ttcatatcca tttctattat gtcatagtct ctaggtattt taccttctaa
    30721 ttcgcattta tcttcataaa atttcctaat ttcatttggt gcatctttat aataaatatt
    30781 tataatttta tcagtcgtct tattcaataa aaattcatta atattgttat tcattccatt
    30841 attaccctca tcaattctaa cttgagcatc aacttcaaca aaagatgtta tagaagtcat
    30901 aaatactaaa acagcaatta gtattccact tttgcctttc tttttaacaa tactctcaag
    30961 tctcttttcc aaatttgtct tatcaaactc ggttgtcaaa atcttactat tataattttt
    31021 actaaactta attgacttta taagtgctag tgcatattct tttttatctt ttaaagaaca
    31081 ttctaccaaa acactttcat cacaacataa ttcacaatct agatttactt tattgctcat
    31141 tacatataca agtggattaa accaatatat tattctaaca gacaaaacta aaaactttat
    31201 taaaatatct ttatttttaa aatgcattaa ttcatgtttc aaaatccaag ttagttcatc
    31261 tttactataa ggatagtctg gtaagaatat gtaagattta aataatccca tacctgctgg
    31321 acttatcaat tcatcagaac ctcttaattc aattttcttt tttatattta attcttttaa
    31381 caagtttttg tagatacagt ttatatcatt atcttcaata tcataagata catcaacaac
    31441 tagattttta aatcttgtat actttataaa tgtataaact gcaatgacta tagttacaaa
    31501 taaccataaa taagctacat acagcataag attattatct gtagaaatat caatttgtgt
    31561 aatattattt cctaatgcat tttctttggg ttcttttact tcatacacta ttgtataatt
    31621 aaatagaaaa agtaacattc taaagactat tatcaaccaa atataataat tgaatctctt
    31681 actaaacttc ttaaataaat atcttttcaa aaataataat agacaaatac caagactaca
    31741 tacaatagtt gtctttataa gattttcaaa aacatatagc atataattat tcatccttta
    31801 aattatcaaa ataactttct agaaaatcta gcttatcttt acctatattt tcatcatcat
    31861 gtaatgctga aattaagctt cttaaagagt catcatgtat attacttagg aaatcctttg
    31921 tctcaaaact taaataatct ttgtgtttta ctcttattgt gtaatgtgta tatctgtcta
    31981 ttttttgagc atctaagaat cctcttttta ccagcctaga taaaagtgtc agtgtggtgg
    32041 tttgtttcca gccatatttt tgttccatag cttcaataac atcttttgat gtcactgtaa
    32101 catctacttt ccaaatgaat ttcattactt ttagttctgc ttgtggtatt tttcttatca
    32161 ttatttacac ctccctcaaa actcctgcta ccttaattct acaacatgtg aattttttag
    32221 tcaaacactt tatgaaaaaa atgtgaattt ttgtactttt tgttaaattt ctaatttttt
    32281 tacaatatta aaagttagat tttaaaatat atctatttaa atattttact ttttacatat
    32341 ttaattacaa tagtgtagaa tgttatttac acattaaaac tatttaataa aaattcctat
    32401 attttcacac aaggaatatt ataatacttt tttttaaaat aaactagagt aatatctaat
    32461 aaattttaat aacattctaa ttatgccttt atgctattaa aacatctatt ttatactgat
    32521 tatttacaaa tatatctaaa ttaattataa attttattga ataagacttt tataattata
    32581 cctttcataa attataaaaa agtactttta taaaaaaatg tgcttttaat atctactaat
    32641 aaagttcttt attttaatta aaacataatt atattttagt attataatct tttaatttta
    32701 gcattatagt ttatgtatta taccctaata tattttattt taaattacta gttagtcact
    32761 cttttcgggt tatttatctc ctatatatta aagtgtcaca tctaaatagt taaatcatta
    32821 tcctctttta tctttattgc ctttacaaat ccatctgcaa ttacaaaact cataagagaa
    32881 gttataaaat atacaaatac tggaacagta aaataaaatc caaatcctaa atctagaaat
    32941 ctcattcctt ttccagtagt accaaaagtt gaaataaact caattagaga gtttaaaagc
    33001 aaaagatatc caataatcct aaatcttttt acattctctt ttacaaatag gctatattca
    33061 gaactaccta atacttctat caatgatttt ataatgataa aatatgatac acctaaaata
    33121 aacattaata ttccataaat agtaccttgt agagttgctt ctccactaaa acagaggtac
    33181 ataaaacttt taaatcctcc tgcaacaatt actaatccta aaagaaaaaa taaaatttgt
    33241 aatataccac ttagtaaatt ttttataagt ttattagaca tcatgcca
//
LOCUS       DAYAOX010000018        29586 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.018, whole genome shotgun sequence.
ACCESSION   DAYAOX010000018 DAYAOX010000000
VERSION     DAYAOX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.018"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(131..943)
                     /locus_tag="ACGI57_000720"
     CDS             complement(131..943)
                     /locus_tag="ACGI57_000720"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9715806.1"
                     /translation="MKICITVGHSILKSGACTSADGVINEYQYNKSLAPVLADTFRKE
                     GHKVDVIICPEKQFKTKNEEKSYKIPRVNSGGYDLLIELHLNASNGQGKGSEVLYYSN
                     KGLEYATRICDKLGTVFKNRGAKLDKRLYILNSSKPTAVLIESFFCDNKEDYDKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVVGGVACEKIGSMTKEKFTMIKGNDRFDTLYKALEFINK"
     gene            complement(943..1374)
                     /locus_tag="ACGI57_000721"
     CDS             complement(943..1374)
                     /locus_tag="ACGI57_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFG9715807.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQNWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKKLKDRIGNLLDTMTDELNIKGGSK"
     gene            complement(1436..1618)
                     /locus_tag="ACGI57_000722"
     CDS             complement(1436..1618)
                     /locus_tag="ACGI57_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715808.1"
                     /translation="MNINNIVVRILAERILSGGLNPLKNREFELDDVTNTEYRKAVED
                     YIIEHSGVVEGTEPTK"
     gene            complement(1618..1911)
                     /locus_tag="ACGI57_000723"
     CDS             complement(1618..1911)
                     /locus_tag="ACGI57_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715809.1"
                     /translation="MNRANRIIYDQTGKILLQTGEATGDILEHDIITQLHCIDVPYGS
                     IDYEKYRIIGINIETKEPISEEIPVYVNEEEKRIQELENQLLIAENEKVGGIL"
     gene            complement(1932..2615)
                     /locus_tag="ACGI57_000724"
     CDS             complement(1932..2615)
                     /locus_tag="ACGI57_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715810.1"
                     /translation="MTTKLTDNASLGELMTALQSVQTDFQTGKNNLSSALGNPFVGTD
                     KLETTKTKIETLKNSLAVNLTNKNVSANETESIQSLINKVGNIKISNYAAGTTSVVKN
                     TSLYAKTFYLNSTSNPSYWISITNLNFLPNVFIAECEYTTSDKWNKYYVIATSYFPSS
                     TVILTDFVLNVSFNCEFSASTLNSGASKLYENNKRDVYITGGSIRVPAFTESSSTLSS
                     YKWYALKIQ"
     gene            complement(2629..3435)
                     /locus_tag="ACGI57_000725"
     CDS             complement(2629..3435)
                     /locus_tag="ACGI57_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9715811.1"
                     /translation="MATDKSYYTILTDIGKAKIANASLVGEKVDFAKIQLGDGGGNEY
                     NPTEEQTALKNVVWEGNVGNVKTDEAMANCLILESLIPANAGGFIVREIGYLDTEGNL
                     LAISKYRSAYKPKVEDGAVIDMKVKTIFVVSNVNNIELKIDPTIIFATLKDLQDLDTK
                     IDTTKTELTSNIEIAKTELNNKIGDTTLLETTDKTSLVGALNEVKTSVDSIETTAEKT
                     SIKDTDNLFESDNVEGALKELATKQNLLDTEVNGQRKKGINIANNLIDMI"
     gene            complement(3451..4068)
                     /locus_tag="ACGI57_000726"
     CDS             complement(3451..4068)
                     /locus_tag="ACGI57_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFG9715812.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFNEVHTFVSSIKPCNMSLDYTRVLVSKDNG
                     MYFGATTVTGHEITIYPWSPSDIETQGEIKIMSGNGFGYQEITIY"
     gene            complement(4061..5110)
                     /locus_tag="ACGI57_000727"
     CDS             complement(4061..5110)
                     /locus_tag="ACGI57_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFG9715813.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEGIYKMKCETAGPIFELGQLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKIQDIGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDITNTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            complement(5111..5530)
                     /locus_tag="ACGI57_000728"
     CDS             complement(5111..5530)
                     /locus_tag="ACGI57_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFG9715814.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLHIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNIKGKVHCKFMVHTKYGNIKAEKVVSV"
     gene            complement(5535..5795)
                     /locus_tag="ACGI57_000729"
     CDS             complement(5535..5795)
                     /locus_tag="ACGI57_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HFG9715815.1"
                     /translation="MSQELLQIIKKAAMDAVETSNPMQIAFGTIESVNPLVVKIEQKA
                     SFEEFFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            complement(5809..7776)
                     /locus_tag="ACGI57_000730"
     CDS             complement(5809..7776)
                     /locus_tag="ACGI57_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9715816.1"
                     /translation="MSDDLVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGAPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYNNKTASELVKMLTKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFETVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLIPVFLDLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNANNKDEKKNGPAQNTTSKEDNDMINKLNKVFKGKLSNTGNIFVKYSNAYK
                     VNPALMAAISMHESARGTSNIANTKNNFFGMKGMSFGSVDEGIKRGISNLSRNYIHIG
                     RKTLESIRDKYAPLYDSPLNKDWVPGVGKFYKQITGNAYSSNSAGTGVGSNEEAEKNL
                     KDTTYQVQNSNNANTSTNNNSKVSKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAF
                     KRGAGINLKRVSADQSKDSRGKLLCNINDVKAGDLVFFKNEQGKVHHVGLYIGNDQYI
                     HAPQTGDVVKISSLSGRQKKKHDFARARRFF"
     gene            complement(7769..8464)
                     /locus_tag="ACGI57_000731"
     CDS             complement(7769..8464)
                     /locus_tag="ACGI57_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFG9715817.1"
                     /translation="MAYDFYLDGVQLPIAPGKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYKGAFKDVQFFLSKLELLKTDCKPFQFIVSRQLGNKVLFN
                     TNIKVSLEEYNIVEDAENGSDTKVAIKLKQYRDYSTKKLVPATPKTTSDAGRPNVKIE
                     KKRVDSVNEPNTKTYTVKAEDSLWAICQKQLGNGSLYKKVYELNKSMMDKANKGKNLS
                     KYTIYKGQVLRLV"
     gene            complement(8476..10959)
                     /locus_tag="ACGI57_000732"
     CDS             complement(8476..10959)
                     /locus_tag="ACGI57_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFG9715818.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQRELRNPLD
                     EGGIQASVKIFDRMAPALKNMNTSINATAKSLERLQQKLNNPINAGNIQASQQNLKNI
                     ESILTRIEQKIGRNTNEQENFNNKIRKGSEEGSLLVSKLKSLAGVYIGIRGIESITKA
                     ADTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAAQVAKLGILAGDAFG
                     SSAEVVKFTELMNKAFVIGGTSASEASAAMYQLTQAMGAGKLQGDEFRSIMENAPLLA
                     TKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFASMPITFSQALTMMK
                     NDAYMIFGQTLGKISGALQSVRFSEIVVSMRNVMIAISSNIYDTLNIIKNILNSDFFS
                     DFVQGITLGAVLIIQGLGGITNAALSVVNVFAQNWNIIEPIVYGVVAAIAIYSLALAG
                     MWLWEKLVAASKFAMMFAQMLLNTKTLAGYAYLIKYAAGQWIANEAMLACPIFWIVAG
                     ILAFIVVVFVAVAAVNKFAGTNLTALGVIVGAVFAAVAFIQNIMIWLFNRCVDVNEGI
                     TNGWNQCVFFMKQAIAKGVIFIIEKMASLNDSVNNAGNALGKAFVTGANIAIRGVNKL
                     IDLLNKIPGINIGKVGEATFTPVKADNSYIKQQINSLNRWVGDAPEKIKLERMQYKDI
                     GAEFQKGNALGTKWQNAISNKLKDTFDINKMLEDAKDKLGLKDLWDKNNPLNNLGGFG
                     GDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQEVINRFTGVNIKIDMNNTNNI
                     SKDADVDGIVNILTEKLNDAMVVSAEGVV"
     gene            complement(11028..11789)
                     /locus_tag="ACGI57_000733"
     CDS             complement(11028..11789)
                     /locus_tag="ACGI57_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFG9715819.1"
                     /translation="MGLFRKSKEPCCICGKEKTDQKIKDGSVCIECLMNYMEYKDKQF
                     TFKDGLPTKSEIKSVLDSKKQNDFLVEKFNVTKEINSFIKFDETNKLIYIETKRKNGK
                     IKKNVYSFEDITGFELLEDGETVTKGGIGSALAGGVLFGGTGAIVGSVVGKKKTKKII
                     ENLQIKLTLKAISEPVAYINLISGKTKTNSIGYERAYEDAQEILSVLSIILKDTENNS
                     NNSNADEILKYKNLLDLGAITEEEFNIKKKELLNL"
     repeat_region   11915..12142
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=11980..12010
                     /rpt_unit_seq="atttacataccacatagttaatataaaataa"
     gene            complement(12420..12578)
                     /locus_tag="ACGI57_000734"
     CDS             complement(12420..12578)
                     /locus_tag="ACGI57_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715820.1"
                     /translation="MDNFLQGVLASLVASLIVYLTSKLFKKVKSHSNTTKSDFDFKLT
                     IKFKRNKH"
     gene            complement(12901..13341)
                     /locus_tag="ACGI57_000735"
     CDS             complement(12901..13341)
                     /locus_tag="ACGI57_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9715821.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKPIEWELKAIDSD
                     RDRQLRKNSAVRVPVIGKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            complement(13343..13600)
                     /locus_tag="ACGI57_000736"
     CDS             complement(13343..13600)
                     /locus_tag="ACGI57_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715822.1"
                     /translation="MVKIKDKYEIKDSISFDYSKKRPIEECVGEMYRKAGLFLVDIAD
                     KLALDTIEGSSLKPITIKIKLDEDGIATIEKETKYLIMEVE"
     gene            complement(13653..14129)
                     /locus_tag="ACGI57_000737"
     CDS             complement(13653..14129)
                     /locus_tag="ACGI57_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFG9715823.1"
                     /translation="MSKNITMNVRDAISGSKAECFVTIKGKRYNFMQAINLEAKMEKN
                     KSEMPILGSITKGNKSTGSKFSGSATFYYNTSIFRELLYEYKETGEDIYFDMQITNED
                     PTSSVGRQTIILEDCNLDSGIIAKFDADGEYLDEDVDFTFENWRIVEKFNIANGME"
     gene            complement(14148..15458)
                     /locus_tag="ACGI57_000738"
     CDS             complement(14148..15458)
                     /locus_tag="ACGI57_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFG9715824.1"
                     /translation="MALGGGTFVAQNKVLPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSTKYFGYDYTHDKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CSIATAKYSGTRGNDLKVIVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTAEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKNDADYEGVVSVENKIKDTGLLESSLVYWTTGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSIRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMGKLYMTVSVS"
     gene            complement(15459..15644)
                     /locus_tag="ACGI57_000739"
     CDS             complement(15459..15644)
                     /locus_tag="ACGI57_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715825.1"
                     /translation="MIILSKTLSKETDYKFTKEQIVNSKKYVNRKDLLNAILRENELY
                     SFSEVEKIINNFMKGVS"
     gene            complement(15628..16065)
                     /locus_tag="ACGI57_000740"
     CDS             complement(15628..16065)
                     /locus_tag="ACGI57_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFG9715826.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYLSGRTLKKNSFDVHYFPKSNDKSFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIVDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            complement(16058..16483)
                     /locus_tag="ACGI57_000741"
     CDS             complement(16058..16483)
                     /locus_tag="ACGI57_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFG9715827.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTTYASYVEYGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            complement(16483..16830)
                     /locus_tag="ACGI57_000742"
     CDS             complement(16483..16830)
                     /locus_tag="ACGI57_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715828.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSCKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(16824..17225)
                     /locus_tag="ACGI57_000743"
     CDS             complement(16824..17225)
                     /locus_tag="ACGI57_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715829.1"
                     /translation="MGNNIIDDIEKRLESFGYILKDGDKWLIDFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLVAYLTTYGKNKILTFRCLRW"
     gene            complement(17235..17468)
                     /locus_tag="ACGI57_000744"
     CDS             complement(17235..17468)
                     /locus_tag="ACGI57_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715830.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(17468..18367)
                     /locus_tag="ACGI57_000745"
     CDS             complement(17468..18367)
                     /locus_tag="ACGI57_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFG9715831.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            complement(18379..18942)
                     /locus_tag="ACGI57_000746"
     CDS             complement(18379..18942)
                     /locus_tag="ACGI57_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HFG9715832.1"
                     /translation="MDWLKELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANEAIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(19012..19260)
                     /locus_tag="ACGI57_000747"
     CDS             complement(19012..19260)
                     /locus_tag="ACGI57_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715833.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            complement(19262..20797)
                     /locus_tag="ACGI57_000748"
     CDS             complement(19262..20797)
                     /locus_tag="ACGI57_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFG9715834.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINNIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRHALVNELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKVFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSNMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     KGE"
     gene            complement(20787..22217)
                     /locus_tag="ACGI57_000749"
     CDS             complement(20787..22217)
                     /locus_tag="ACGI57_000749"
                     /inference="COORDINATES: protein motif:HMM:TIGR01538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9715835.1"
                     /translation="MYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGEEYY
                     YGNADINNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNADDD
                     NLTKLLNDLLGEEFDDTITELYLNASNKGIEWLHPYINRKGEFKYVIIPAEEAIPIWD
                     SKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYGKMT
                     DIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLNFYKSLIDIYDNNISTLADNLDEI
                     QEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRLEKN
                     IIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEYLKI
                     SGSKSYDYKSVQITFNHSMIINESEKIDMAAKSIGIISDETIVSNHPWVEDVNDELER
                     LKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            complement(22232..23485)
                     /locus_tag="ACGI57_000750"
     CDS             complement(22232..23485)
                     /locus_tag="ACGI57_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFG9715836.1"
                     /translation="MVIAMRVRIDFNPSFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKITGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANNKGLEKNLFMYCDSAEPDRIKMWKSAGY
                     EAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(23463..24146)
                     /gene="terS"
                     /locus_tag="ACGI57_000751"
     CDS             complement(23463..24146)
                     /gene="terS"
                     /locus_tag="ACGI57_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFG9715837.1"
                     /translation="MQDVKEKVKQDYIKGMKQKEISAKYDISLNTLKSWIKRYNWASE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETQDLINEIKNKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLSKDDIGDDELKIS
                     VDYGDSNES"
     gene            complement(24209..24415)
                     /locus_tag="ACGI57_000752"
     CDS             complement(24209..24415)
                     /locus_tag="ACGI57_000752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715838.1"
                     /translation="MYYKHFTELVRKKLGIPKGVKRDELNQSELFDIQALERIISMKL
                     PKLIDKDMNYKEVYKKIKELIEMI"
     gene            complement(24468..24887)
                     /locus_tag="ACGI57_000753"
     CDS             complement(24468..24887)
                     /locus_tag="ACGI57_000753"
                     /inference="COORDINATES: protein motif:HMM:NF014548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFG9715839.1"
                     /translation="MLKILQEKNVKVMWSKNGEEVWFNANDVGEELGIVNIRDTLRNI
                     DREYKKKFNESTVGDSYTRNFKDKLPNFGTTFVTEEAVYNMSFRSNKPEAKLFTKWVT
                     QTLKQIRIHGYYIATEKDQEWLDIRTEGKKSKKRFYR"
     gene            complement(25140..25346)
                     /locus_tag="ACGI57_000754"
     CDS             complement(25140..25346)
                     /locus_tag="ACGI57_000754"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715840.1"
                     /translation="MSNLREKIIKELNEFNIDADDEFLSYGVEYVESFIGKSAISEEL
                     LIRGVVFTANYIVNMKAKDGVNKA"
     gene            complement(25786..26268)
                     /locus_tag="ACGI57_000755"
     CDS             complement(25786..26268)
                     /locus_tag="ACGI57_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715841.1"
                     /translation="MNKKTLFQEVEGRLYNYKKLESQIRIKDIYIKKLESEFCGCKAQ
                     SYEEKTGETYNISSSVENEVIKREEDLNRLKEDKKTLEIEKETIECALTSLNSFETEF
                     FNEMYMNNEKINMDYMSNAMHIDRSHCFRIRKRIVCKIMDMLYPKIKEFELPIFSWKA
                     "
     gene            complement(26372..27109)
                     /locus_tag="ACGI57_000756"
     CDS             complement(26372..27109)
                     /locus_tag="ACGI57_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFG9715842.1"
                     /translation="MNNLKLIENEGLIKVYTTDEDIKVVNGRELWEGLGVKQQFSDWI
                     ETNLKNVDANKNEFYILKGKTSKQGGRPTDEYIIKLEIAKEICLVAGASPRANKELKR
                     NSKNYRKYLIAVEEKYKVSNNLTKTQLNQINDIVSNALCEMQTKHDAQIEQFKKESSQ
                     YYRPTSKTKYDISSYIKNRLDIPRANEEFDLVKKRTLLILGADKWEDIPKDVLLKSLN
                     IIDESIRIIKSERKTNQISFFEKDNFC"
     gene            complement(27172..27579)
                     /locus_tag="ACGI57_000757"
     CDS             complement(27172..27579)
                     /locus_tag="ACGI57_000757"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFG9715843.1"
                     /translation="MKVNFVIDGEPVGKERPRMNSITKRTYTPNKTKNYEELIKWLYQ
                     SKVKYRFTGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDNPRVEVILEDIS"
     gene            complement(27569..27967)
                     /locus_tag="ACGI57_000758"
     CDS             complement(27569..27967)
                     /locus_tag="ACGI57_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715844.1"
                     /translation="MQKDIWLYSWDDEYFASDEYESKEEAINGAKEELKRFKEVKQCV
                     YIGKKEDVNIPNIDAEDALERVQDRIDDEFGGFGEDWFENIRAEDILTLENRISKVFK
                     KWIDEFGYKPYWFVVRDTEEIELNEVANES"
     gene            complement(28106..28303)
                     /locus_tag="ACGI57_000759"
     CDS             complement(28106..28303)
                     /locus_tag="ACGI57_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6877 family protein"
                     /protein_id="HFG9715845.1"
                     /translation="MKNKVEINNIQQLNDALNKYNIPFDVLSDIDRRICGWMATGGNE
                     DDAYIKQQYRYVENFINLFCD"
     gene            complement(28399..28728)
                     /locus_tag="ACGI57_000760"
     CDS             complement(28399..28728)
                     /locus_tag="ACGI57_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715846.1"
                     /translation="MKIFLLTILLIIICILANYVKNRIYKKSINNLKYKYSVGEKIIY
                     HQINCYYNRMVGCEILEKCYSTKFRKRNTPLYKVKAYVGDNNTTWVIPEWRIECLATT
                     YGEFPKY"
     gene            complement(28810..>29586)
                     /locus_tag="ACGI57_000761"
     CDS             complement(28810..>29586)
                     /locus_tag="ACGI57_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFG9715847.1"
                     /translation="KNSKEIASYLFDVMKINAALESCDLAICLYTIKDEKSVAILKLD
                     YKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALISLSGINDEFHLRLLDKDAE
                     KEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNALSNDIKQAEDVRSILNYTL
                     KEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSIDKKWVEKKLKKRSIKTDN
                     GFDIKGNLTDFEDPMKYTVRQNQNGSIDIIIKNVTFYEEK"
BASE COUNT         8638 a         5661 c         3127 g        12160 t
ORIGIN      
        1 tatacctgat tttttaaagt ttgtagatga cttatggtta tatttaatat atcatttttt
       61 attttataaa ttgttgtgat actgtcataa aattgtagtc aaaatgtaaa atatattaac
      121 tctattgatt ttatttattt ataaactcca gtgctttata aagtgtatca aatctatcat
      181 taccctttat catagtaaat ttttctttag tcatagaacc aatcttctca catgctacac
      241 cgcctacaac ataaagattt tgcgtctgac ctggcacgta atcttttata tcacatatca
      301 gtattttccc atcattataa ccccaaccaa ctacagttgc agagattttg tcaacttctc
      361 catcataaac aattgtatgt ttgtacatct gtttaactcc ctcattattt atatttttat
      421 ttaatacacc ttctacaatt aacttagcaa taccttcatg acctagtttc ttagctttat
      481 cataatcttc tttattatca cagaagaaac tttcaattaa tactgctgta ggctttgaac
      541 tatttaagat atataatctt ttatctaatt tagcacctct atttttaaat actgtaccta
      601 gtttatcaca tattctagtt gcatactcta agcctttatt actataatat aggacttctg
      661 aacctttacc ttgaccgtta cttgcattta aatgtaactc tataagtaaa tcatatcctc
      721 cactattaac tctaggtatt ttataagact tttcttcatt cttagtttta aactgctttt
      781 ctgggcatat tattacatct accttatgcc cttcttttct aaatgtatct gctaatactg
      841 gtgcaagaga tttgttgtat tgatactcgt taattactcc atcagcagaa gtgcatgctc
      901 cactttttaa aatactgtgt cctactgtaa tacatatttt cattatttac tacctccttt
      961 aatatttaac tcatctgtca tagtatctaa taagttacct attctatctt ttaatttttt
     1021 aggcactggt aacccacaca aatacatgtt ttttaatata cttacacttt catatagaat
     1081 aaataaaata gagaaaaatt cagatattcc aagatgattt aatcgtaaaa aatcaaccca
     1141 attctgcggt aacataaata aaaaattaaa ctttgtaagt atgtcaacta ctgctagaaa
     1201 aaatatacat gctatcattg caacttttct tattcctcca tttattccaa aagagctatt
     1261 aaactgatgt gtttttattg cccttaagca acctagtaat gtatcaaatg ctattgctaa
     1321 tattactaat tttataaata tatttgttgc taaaaaaact attgttacgt tcatatttcc
     1381 tccttatttt gcattaaaat aagacttaga attatctaag cctataaaaa agaacctatt
     1441 ttgtaggttc tgttccttct actactccac tatgctctat aatataatcc tctactgctt
     1501 ttctatactc tgtgttagtt acgtcatcta attcaaattc tcgatttttt agagggttta
     1561 aacctccact taaaatcctc tctgctaata ttcttactac aatattattt atgttcatta
     1621 taaaattcct cctacttttt cgttttctgc aattaataat tgattttcta attcttgtat
     1681 tcttttttct tcttcattta catatactgg tatttcttct gaaattggtt cttttgtttc
     1741 tatatttata cctattattc tatatttttc atagtctata cttccatatg gaacatcaat
     1801 acaatgcaac tgtgttatta tatcatgctc caatatatcc cctgttgctt ctcctgtttg
     1861 caagagtatt ttacctgttt ggtcataaat tattctattt gctctattca ttttatcacc
     1921 tcatttttaa tttattgtat tttaagagca taccatttat aagaagatag agtagaacta
     1981 gactctgtaa aggctggtac tctaatactt cctccagtaa tgtaaacatc tcttttatta
     2041 ttttcatata actttgaagc accactgttt agagtactag cactaaactc acaattaaat
     2101 gatacattta atacaaaatc agttagtata actgtggatg aaggaaaata agaagtggca
     2161 ataacataat atttattcca tttatcactt gttgtgtatt cacactcagc aataaaaaca
     2221 ttaggaagaa agtttagatt agtgatacta atccaatagc taggattact tgtgctgttt
     2281 aagtagaatg ttttggcata taaactagta ttttttacaa cagatgttgt tccagcagca
     2341 taattagata tttttatatt tccaacttta tttattagac tttgtatgct ctcagtttca
     2401 tttgcagaaa catttttatt tgttaaattt actgctaaag aattctttaa tgtttctatt
     2461 tttgttttag ttgtctctaa tttatctgta ccaacaaaag gattacctaa tgcactagat
     2521 aggttatttt taccagtttg aaaatcagtt tgtacacttt gcaatgctgt cataagctcc
     2581 cctaaactag cattatcagt taattttgtt gtcatatttt cacctcactt atatcatatc
     2641 tattagatta tttgctatat taattccttt tttcctttgt ccatttactt ctgtatctaa
     2701 caagttttgt tttgttgcta attcttttaa tgctccttct acattgtcac tttcaaataa
     2761 gttatctgta tcttttatac ttgttttctc tgctgttgtt tctatactat ctacactagt
     2821 ttttacctca tttaatgcac caacaagact agttttatct gttgtttcaa gtagagtagt
     2881 atcacctatt ttgttgttta gctctgtttt agcaatttct atgttgcttg ttaattctgt
     2941 cttagttgta tcaattttag tgtccaggtc ctgcaaatct tttaatgtgg caaagattat
     3001 tgttgggtct atcttaagtt ctatattatt tacattagat acaacaaaga tagtttttac
     3061 cttcatgtca attactgctc catcttccac tttaggttta tatgctgacc tatactttga
     3121 tatagcaagt aaattaccct ctgtatctaa ataacctatc tctctaacta taaatcctcc
     3181 agcatttgca gggataagac tctctaaaat tagacaattt gccatagctt catcagtttt
     3241 aacatttccc acattgcctt cccaaactac attcttaagt gctgtctgtt cttctgtagg
     3301 gttatactca tttcctccac catcacctag ttgaatttta gcaaagtcaa ccttttcacc
     3361 tactaaactt gcatttgcaa tcttcgcttt tcctatgtct gttaatattg tgtagtaact
     3421 tttatctgta gccaaactac cacctcctat ctaataaatt gtaatctctt ggtatccaaa
     3481 tccatttcca ctcataatct taatctctcc ttgtgtttca atatcactag gtgaccaagg
     3541 gtaaattgta atctcatgcc ctgtgactgt tgttgcacca aaatacatgc cattgtcttt
     3601 actaactaac actctagtat aatcaagact catgttgcaa ggtttgatgc tacttacaaa
     3661 tgtatgaacc tcattgaacc aatcttgatt tctagcatca ctctcaaggt gtatgttata
     3721 agtagcatta ttaatagtta attcataatt gccttctcca actacattat ctagccagtt
     3781 ccttaaaaat ctctctgagt aaggtaattt acttatatat ttactaaaaa tcctaaacct
     3841 tctatcttct aaactctcat tacttttagg agttatagac attatctttt cccatctttt
     3901 tataccactt atagttaggt cctctaaaaa ctggtcattt gataggtcct ttaatttatc
     3961 atgtagtgtt tttatttctt tattttctac attaaatact tttatatatt cttctttatc
     4021 ttgtagaatt tgtggcaagt aatttattag attaatctct ttatccaact acctcacctc
     4081 tcactactat actgttacta tctattgtta gattagattt aacatcattt atcattgtgt
     4141 ttgtaatatc taacactcca tctatactaa gtaatctagt ttcaatttga gatatacgga
     4201 ctattaagtt ttcttcatct tcccaactca tgttaagttc atttaaatag tcgtctattg
     4261 cttcttctgc aattgatttt atattctccc atgtatatcc actcttatat gttatttctg
     4321 ctgatatatt tatagttgta cttgtaactc cttcaactgt gactctatga cctattggtg
     4381 ctaatcctaa gccttgtcct tgatgtccaa ttgggtcaat ttcttcttgc actaaattaa
     4441 ctaaatcctc tgatggtact ttgaaattag aattaattat tactaactta acagtacctc
     4501 caccgttcca aactggataa actttaactc ctccaatatc ttgtattttg ttaacttcat
     4561 ccttatagtt ttgtatattt ccaccgaaac tttgtgaatt taaactatca tagtaccttt
     4621 gtcttaaact gtcctcgctc tcttcatctt ctccatttat cagtatttct gttaactctg
     4681 ctgtttcaag accgtctata tattcaattg gaatcaactg accaagttca aaaataggtc
     4741 cagcagtttc acatttcatc ttatatatac cttcagatat tctctcaatt gctacatagt
     4801 tatactctcc taaattaaac ctagaatcaa gtggaatatc tatattgaaa acgcctttag
     4861 caatcgtatt agtggctggc aaaggtgtaa ttcctctctc tttacatctc ttctctaaat
     4921 agtaataact agcagtatct acaaatgttt ggtctagtaa ttcatccatt gcaatgtatg
     4981 tttctgtaag ttctatagca actggtgcca aggcattata tattatagaa ccttctcttt
     5041 tatcaaaagt atctggtaca ctatctaaca ttcttttaat tatattttca aatgtcatta
     5101 actcaaacaa ttatacactc accaccttct ctgcttttat atttccatat ttggtatgga
     5161 ccataaattt acaatggacc tttcctttta tattttgaaa ctcaaaatta tctacatttt
     5221 caaccctatc atcttgaatt agtgcttctt tgattcgtct ttcaagttcg ggtattacaa
     5281 aggatatagg ctctccaata aggtcgttca actcgactcc ataatttcta ctgtatatta
     5341 aatgctcata cctttcagta tttaaaatca aaaagatggt ttgttttaat gcttccacat
     5401 catcacaaat accatctact ctgttttttt ctatatgaag tttaaatgtc ttacttggtt
     5461 cttgccttac atcaaaattt ataattgata catcttcaat atcatagtca atgttatctg
     5521 ttggtaacac tttatcacat cctatctaaa atcaagtatt gttgtcctcc ttgcatacga
     5581 attaaaacca atttatcccc tattttttta tctgtatatc ttttgaatgt atctgtttgt
     5641 attagaaaaa attcttcaaa agatgctttt tgctctatct taactactaa aggattaaca
     5701 ctttctatag ttccaaatgc aatttgcatt ggattacttg tttctactgc atccattgct
     5761 gctttcttga ttatttgcaa taattcttgg ctcattttat cacctcgctt aaaagaatct
     5821 tctagctctt gcaaaatcat gctttttctt ttgtctacca cttaaactac ttatttttac
     5881 tacgtcacca gtttgtggag catgaatata ttggtcattt cctatataaa gtccaacatg
     5941 atgtactttt ccttgttcgt ttttaaagaa tactaaatca cctgctttta catcatttat
     6001 gttgcataat agtttccctc tactatcttt tgattggtct gctgaaactc ttttgagatt
     6061 tattcctgca cctcttttaa atgcccacac cataagacca ctacagtcaa aactctttgg
     6121 accattacca ccccatttgt aaggcttgcc aagttgattt tttgcttctt gaataacttt
     6181 acttacttta ctattattgt ttgttgatgt attagcattg ttactatttt gaacttgata
     6241 agttgtatct tttaaattct tttctgcttc ttcattgctc ccaactcctg tacctgcact
     6301 attagaacta tatgcatttc ctgttatttg tttataaaat ttgcctactc ctggtaccca
     6361 atctttatta agaggactat cataaagtgg agcatattta tctcttatac tttctaatgt
     6421 tttccttcct atatgaatat aatttcttga taaattactt atacctcttt ttattccttc
     6481 atctacagag ccaaaagaca ttcctttcat tccaaagaaa ttatttttag tatttgcaat
     6541 atttgaagtc cctctagctg attcgtgcat agatatagca gccatgagtg ctggattaac
     6601 tttataagca tttgaatatt taacaaatat atttcctgta tttgataatt tacctttaaa
     6661 tactttattt aatttattta tcatgtcatt atcttctttg cttgtagtat tttgtgcagg
     6721 accatttttc ttttcatctt tattatttgc atttccacta ctgtagcttg aagaagaata
     6781 agaagcaaat tcatctccat caacaagagt taaatccatg aaatgtgaat tattttcaaa
     6841 tgtatgtttt actttctcaa ctaacatata attttgtaac tcaatatctc ctaaatctaa
     6901 aaaaacaggt attaaacaac ctgctcttac tctaatatct cctaacacat tttttaaact
     6961 taatgactta gttttcttat tatataattt taaaagtata tcacactttt gttttatctc
     7021 tgcttcactc atgtttttat caactgtctc aaacatttga agtattcccc aacttctcat
     7081 atgtgctgag tcttgagcaa tatacacatc tctttttcct gactcctcgt tgtctctgac
     7141 gagtttaatt tttgtataag tatcactatc tatacttgaa ttatagtcaa aatcctctat
     7201 gacatcatta ttcataacag tatctaattt cattgatgca acattcttta atgttattct
     7261 tccaaaatca tcatagagta catacatttc ctttttctct cttagagtat catctagtgc
     7321 tgttagtatc atgtcaaata aagttttatt ttcttcaact ctcgatattt tatattttgt
     7381 gtcttctatg acattgtatt ttaagttaaa atctttagtt agcatcttta caagttcact
     7441 tgctgtttta ttattataca cataagtatc tttattctta aaatatctca actggtcgta
     7501 agcaacaatt ttaatatgat tttctttatc tcttttcttt tgaaatatat atccataaaa
     7561 tatacctatt cccttataat acagccttac agagttacct tcacaaaact ctaatatatc
     7621 atccatgact attgtaaatt ctaatttact tggcgctcct cgtctttcta tctcccatgt
     7681 gattccatct aaaacgacag gctcatagaa atcttcccaa tgtgctatga ctaatcttac
     7741 atctctatca tttgctaaca ctaaatcatc agacaagcct caacacctgc cctttgtaaa
     7801 tagtatattt acttaaattc ttccccttat ttgccttatc catcattgat ttatttagtt
     7861 catatacttt cttatatagt gaaccatttc ctaattgctt ttgacaaatt gcccaaagag
     7921 aatcctctgc ttttactgta tatgttttag tgtttggttc attaactgaa tcaacacgtt
     7981 ttttctctat ctttacgttt ggtctaccag catcactagt cgttttaggt gtagcaggaa
     8041 ctaatttttt agttgagtaa tctctatatt gctttaactt tattgcaact tttgtatctg
     8101 agccattctc tgcatcttct actatgttat attcctctaa tgatacttta atattagtgt
     8161 taaatagtac tttattccca agttgtcgag atacaataaa ttgaaatggc ttacaatctg
     8221 tctttagtag ttctagttta cttaaaaaga attgaacatc tttaaaagca cctttgtaaa
     8281 acggtaattt attatgtgta aattctgctt caaaacttat ttcagatagc ccttcctttt
     8341 ttaatatgtt tacttctcca gtatttatca aatcaactgt cttattcttg tttgttactt
     8401 taatttcaag tttaccaggt gcgattggta attgtactcc atctaaataa aagtcataag
     8461 ccatttatat ccctcctaaa ctactccctc agcagataca accatagcgt catttaattt
     8521 ttcagttaaa atattgacta taccatccac atctgcatct ttacttatgt tgtttgtatt
     8581 gttcatgtca attttaatgt ttactcctgt gaatcggttt attacctcct gctctgcaat
     8641 atctctaaga tattttaaat cttcttgact tttatccatt gttttagcca tttttgcagt
     8701 gtttcctgcc gtgtccttta cattttttcc taaatctcca ccaaatcctc caagattatt
     8761 aagtggattg tttttatccc acaaatcttt taatcctagc ttgtcttttg catcttctag
     8821 catcttgtta atgtcaaaag tatcttttaa tttattagat atagcatttt gccatttagt
     8881 tccaagtgca tttccttttt gaaattctgc tccaatatct ttatattgca tcctttccaa
     8941 ttttattttt tctggtgcat ctcctaccca tctgtttaaa ctgttaatct gttgcttaat
     9001 gtaactgtta tccgccttaa caggtgtaaa tgttgcctct cctactttcc caatgttaat
     9061 cccagggatt ttatttagca aatctattaa tttatttaca cctcgtattg ctatattagc
     9121 tccagtaaca aaagccttac caagtgcatt acctgcatta ttcacagagt cattcaatga
     9181 tgccattttc tcaattataa agattacacc ttttgcaatt gcttgtttca taaaaaaaac
     9241 acactgattc caaccatttg taatgccttc atttacatct acacatctat taaacagcca
     9301 tatcattatg ttttgtataa atgctactgc tgcaaatact gcacccacaa ttactcctaa
     9361 ggcggttaaa ttcgttcctg cgaatttgtt tactgctgct actgcaacaa aaactactac
     9421 tataaatgct agaatgcctg caactatcca aaaaatcggg catgctaaca tagcttcatt
     9481 tgctatccat tgtccagcag catattttat aagatacgca tatccagcta aagtttttgt
     9541 atttaaaagc atctgagcaa acatcatagc aaatttagat gctgcaacta atttttccca
     9601 taaccacatt cctgccaaag ctaaagaata aattgcaata gctgcgacaa ctccataaac
     9661 aatcggctct attatgttcc agttctgtgc aaaaacatta acaacgctaa gtgcagcatt
     9721 agtaattcct ccaagccctt gtattattaa tactgctcct aatgtaattc cttgaacaaa
     9781 atctgagaaa aaatcactat ttaatatatt ttttataata tttaatgtat cataaatatt
     9841 tgaagatatt gcaatcatta cattccgcat agacacaaca atctcactaa atctaacgct
     9901 ttgcaaagct ccacttattt ttcctaatgt ctgaccaaat atcatataag catcattttt
     9961 catcattgtg agagcttgag aaaaagtgat tggcatactt gcaaatttct tttctatctc
    10021 atcagaagct ttaaacagtg catttcttat aacatctgct gttattgctc cactacttga
    10081 taattccttc aattggtctt tagttttccc cattgcatca gctattttag tagctagaag
    10141 tggagcattt tccatgatag aacgaaactc atcaccttga agtttcccag cacccatagc
    10201 ttgagtaagc tgatacattg ctgcactagc ttcacttgct gatgtccctc caattacaaa
    10261 agctttattc ataagttctg taaactttac cacctctgct gaacttccaa aagcatctcc
    10321 tgctaatatc ccaagtttag caacctgtgc tgctgtatct gcataactag ctcttgcact
    10381 ttgagcagac aaataaatca tcttattcag ttgctctgtt gtctgtaagc catcattcat
    10441 tagatttaaa cgtgcttttg tactggcaat tgtatctgct gcttttgtaa tactttctat
    10501 acctcttatt ccaatatata cacccgctaa gcttttaagt ttagatacta atagagaacc
    10561 ctcttcactt ccttttctta ttttattatt aaagttttct tgttcatttg tatttcttcc
    10621 aattttctgt tctatcctcg ttaaaatact ttcaatattt tttaagtttt gttgtgaagc
    10681 ttgtatatta cctgcattta taggattgtt caatttttgt tgtaatctct ctaaactttt
    10741 agctgttgca ttaatagatg tattcatatt tttaagtgca ggtgccattc tatcaaaaat
    10801 ttttacagat gcttgtattc caccttcatc tagaggattt ctcaattctc gttgtaatcg
    10861 ctctaaacta ttaatcgttg tgttaatgga tgtagtcatg tttctaaatg caggtgtcat
    10921 tccgtcgaaa atctttattg aagtttgtat tgtagccatt tcctcactct cctttctttt
    10981 ttgaaataat aaaagcactt acttattttt aagtaagcgc ttatgtatta taaattcaat
    11041 agttcttttt tcttaatatt aaattcttct tctgttattg ctcctaaatc taataaattt
    11101 ttatatttta atatttcatc tgcattagaa ttattgctat tattttctgt atcttttaaa
    11161 attatagata aaacagataa aatttcttgt gcatcttcat acgctctttc atatccaata
    11221 gaatttgtct ttgtttttcc actaattaaa tttatatatg ccacaggctc tgaaatagct
    11281 tttaatgtaa gctttatttg taaattttca attatctttt ttgttttttt cttgcctaca
    11341 acgctaccaa caatagctcc tgtaccacca aacaagactc ctcctgctaa agcacttcca
    11401 ataccacctt ttgttacagt ctctccatct tctagcaact caaaacctgt tatatcttca
    11461 aaactataaa cattcttctt aatttttcca ttttttcttt tagtttctat atatatgagt
    11521 ttattagttt cgtcaaactt tataaaactg ttaatttctt tagttacatt aaatttttca
    11581 actaaaaaat cattttgttt cttagaatct agaacacttt taatttcaga ttttgtaggt
    11641 aatccatctt taaatgtaaa ttgtttgtct ttgtattcca tataattcat tagacattct
    11701 atgcaaactg aaccatcctt tattttttgg tctgtctttt cttttccaca tatacagcac
    11761 ggttcctttg attttctaaa taatcccata atattacccc catataataa ttttataaga
    11821 ttattatact atataagtaa aatttttaca tcataatcac ctcctttcaa taaaaaaaca
    11881 cctactattc aagtaagtgt ttttagcgtt tttaatttta agtccacata gttaatataa
    11941 aacattttta attgaagtcc aaatttgaga aaatgtgtaa tttacatacc acatagttaa
    12001 tataaaataa ttatgggtag attttaaaga taaagtaaaa aatggattta cataccacat
    12061 agttaatata aaataaggta atactgtaat agaaccagaa tttgattatc tatttacata
    12121 ccacatagtt aatataaaat cccaaaataa acttagcatt tccaatacct acacatacgc
    12181 atctctctca aatttgcagt gaaccatgag tagtgcaatt gataacattt atcacacacc
    12241 ctcaacgcct tatatttcaa tgcttaagcc ttaatttctt caaaaaatcg aacactgcaa
    12301 aatttctatg cctttattat atcataaaaa tattattttt gaatatctgt accaatttgt
    12361 ggtataataa aagcaagaag aactacaatc tatttgaaac tagagtgaag ttctaaaaat
    12421 taatgtttat ttcttttaaa cttgattgta agtttaaaat caaagtcact cttagtcgtg
    12481 tttgagtggc tttttacttt tttaaataac ttactagtta agtaaactat taaactggca
    12541 actaaacttg ctagtacacc ttgtaaaaaa ttatccatac atactcacct cccttctata
    12601 cgttgggagg ataatctttt gtatgaactc cactctataa attgtagatt acatcttctt
    12661 gctaaaaata ttataacata taattcttac atattttacc tatattttat cttcttctac
    12721 ttcttctagc ttctttagca gtcttttttt cttcttttat ttcttcttct actttaatat
    12781 ctatagaagc agcaacaaat gccctctcgt agtctggtaa atctgtatat tcatgtggtt
    12841 tccatttgaa tttatgaagg caataatgag ctgcactagc atcataatcg cctccttcaa
    12901 ttagtttttt gcttcttcta ctttatcctc aaaagtccta tcaaaaccat ttacctctcc
    12961 tacttcactt gaaaggtctg tgtattcgcc aggagttaac attgttgtta atagttcctc
    13021 tgctcccatt acaccatagc tattttgaag ttctgcatca tgtaaatctg gaaatactat
    13081 agtttccaca cataatttca aagtgtaagt attaaaatct gtttcactag tgtattgccc
    13141 tgttgctttt ccttttttgc ctataacagg aactctaaca gcagaatttt ttctcaactg
    13201 tctatctctg tctgaatcta ttgctttaag ttcccattct attggtttac catcctcacc
    13261 tataaacctt tcacttgcca catactttct attctctact tttattgcat tttgacttaa
    13321 aaaagcgttt aaatctccca tattattcta cctccatgat taaatatttt gtttcttttt
    13381 ctattgttgc aatgccatcc tcatctagtt tgatttttat tgtaattggt tttaatgatg
    13441 aaccttcaat tgtatcaagt gccagtttat cagcaatatc aactaaaaat agacctgctt
    13501 ttctatacat ttctcctaca cattcttcta taggtctttt cttactgtaa tcaaaagaaa
    13561 ttgaatcttt tatctcatat ttatccttaa ttttaaccat ctcctattct tataaataaa
    13621 aaatacacat ttatgattta tagatgtgta ttttactcca taccatttgc tatattgaac
    13681 ttctcgacta ttctccaatt ctcaaaagta aaatctacat cctcatccaa atattctcca
    13741 tcagcgtcaa atttagcaat tatacctgaa tctaggttac aatcttcaag tattatagtt
    13801 tgacgaccta ctgaacttgt tgggtcttcg ttagtaattt gcatatcaaa gtaaatatcc
    13861 tcgcctgttt ctttatattc atataacaac tctctaaaaa tagaagtatt ataataaaag
    13921 gtagcactcc ctgaaaattt acttcctgta cttttatttc cttttgtaat gctacctaaa
    13981 attggcatct cacttttatt tttttccatc ttagcttcta agttaatagc ttgcataaaa
    14041 ttatatcttt tgccttttat tgttacaaaa cattcagctt tagaaccact tattgcatct
    14101 ctaacattca tagtaatatt tttagacatc tatatcactc tcctttctta actaactgaa
    14161 acagtcatat aaagcttacc catagcacta ataactttta cagcatcact tactacaaca
    14221 gttttcttgt cgcttccagg ttctacagaa acatcatcag ctttgaaatc ttctattgct
    14281 ctcatatttt gcagttgttc atgatgctta actacatcat tccaaaacga gatacgacca
    14341 gatttatcat ttggtacttc gcccaagtac tttgtattaa ataaagtcgc tatatcatta
    14401 gcaatctggt caagtactct aatactttga ttagatgaaa aatcgtcatt cttatcgtct
    14461 gtaaaactta caaaagtatt tatgtcctct aatacatgaa cttcatctcc caccttgtgg
    14521 aatataaatt taccagtttt taaagcttct tcaagttgta tttgagtata attaacatca
    14581 acatcaaact caccatcata cttcttattt gtattagatt tatttatgtc acatcctgct
    14641 atagctccag tagtccaata aactaaacta gattctaata acccagtatc tttaatctta
    14701 ttttctacag atactacacc ttcataatct gcatcatttt tcttatatag tactgtttga
    14761 aacttagctc ctaccttatc tctcattctc tttgtaaatt ctacaaacaa acttttaatt
    14821 tctgctgttg tagccaaaca ccctagtgca ttaaatgaat aactttctat tttatccaag
    14881 aaagcttggt actctgctcc tgtcacagct tcgccattag ttccaccagt aaatacaagt
    14941 cctgcgcttg cttctaatgt tgcatccttc ttccaagtga tatagtcatt atcttgtaag
    15001 tctgtaatca ctttagctat ttgagtatct accttcttat tatctaaaag tgttacaaca
    15061 tcaaacttag tgttatcatc tatatttgtt gttactataa cttttaaatc attacctcta
    15121 gtacctgagt acttagctgt agcaatactg caactagctt taacaccttt attcaattta
    15181 taaaaatatc ccaaccttat atttttaaat aaatctctaa gacctttcaa cttatcatgt
    15241 gtataatcat atccaaaata cttagttgaa tacttctcaa aatcatcact ggttacttga
    15301 aatacttctt catctatgcc ccagtctagc tccaaaggca ttgccacaat acctctatcc
    15361 gataatgaac tggttgccct cttagctgag ataaaattta tatatgcacc aggtaggacc
    15421 ttattttgcg ctacaaatgt tcctccacct aaagccatct aactcactcc tttcataaaa
    15481 ttatttatta ttttctctac ttctgagaag gaatataact cattttctct taaaattgca
    15541 tttaataaat cttttctatt tacatacttc ttagagttaa ctatctgctc cttagtaaat
    15601 ttgtaatcag tttctttgct taatgtctta ctcaaaatta tcacctctct tcaaaccacc
    15661 gaataactct actgtatcca tcttatctgt atcattactt tttatagtaa agtagttata
    15721 atcaacgaag aaatgaagaa cattatctac aatttcaaag ttcatatttg tacctctgac
    15781 taagtctcca tcaatttcta tatactctaa ttcctccagt aacatctcag ctatctcatt
    15841 tatttcaaat gatttatcat tacttttagg aaaataatgt acatcaaaag agttcttttt
    15901 taatgtcctg ccacttagat aggatacttt gcttggattt aaaggaacaa taaaaaaaca
    15961 aggttcattt ataccttgct ccacatcttc actataaatt gtataactct ctccaaatgt
    16021 tttatctaat ttaatagata ttccatcaat tatattatta agcatcaaat actcctttaa
    16081 gcaatattaa tagttttttc tctataatct tatcaacttg gctttgtagt tccatctctg
    16141 aaattgttaa gaaatgttgt cctttaaccc aaccttttcc atctttagtt ctatggccat
    16201 attcaacata acttgcatat gtggtcggat taacaacctc tataatataa ttatttcctt
    16261 gtttatacac aggaagcgac ctagcataag ccactccatt ccatccttgt cgtaagaatc
    16321 ctgtatcaac tggtgttctt ctaattactt tcccaagtaa tcgtgctgct aattctcttg
    16381 ctgcatcctt gcaaaactta tctaaatcaa tctttgtaag ctcctccatc tttttacaaa
    16441 ctcttttaaa ctctctaaaa tcaacactgc cccatctagc cattatgctt tatccttaaa
    16501 taactcaagt attatttctt gatgatttgg atatatagct gattctccac ttcttacata
    16561 ctctttatca tttataataa gttttgaacc tgctttaatt tctatatctg gagatataaa
    16621 gagtttaata gtttgctcta gcttagctac tttcccttct gtagcagaaa ctatattttt
    16681 acatgaaagt ttacatggtt gattttctaa tacaatcact tctttattgt tagttcgttt
    16741 tgttacaggg tctttaattg gctgatactc aactatagta catttatctc tatataacat
    16801 ttctattgct tttctagttt tacttaccat cttaagcacc taaaggttaa tatcttattc
    16861 ttaccataag tagtaagata agctactaag ctatcaaagc gttgttctgg tgtttgagag
    16921 ccacttccta tagcaaaatc tacctttgta tcaccttctg atatagactt ttctacagct
    16981 tcaaagttaa tgctttctat atctaattgc cccatatttt tcttggtaaa taagaactct
    17041 ccaactatca tatcaacttc aatttctttc aattcaattg gcatagtttt tatattacaa
    17101 tctagtttaa taatattttc tattttttct cttacaaaat ctattaacca cttatcccca
    17161 tcttttaata tatatccaaa actttcaagt cttttttcta tatcatcaat tatattattt
    17221 cccataattt tcacctactt tttagtaagt ttatttttct ctttaagctg tttattttct
    17281 tcttctaaag actcaacttt tgaccttaaa atattatttt cagctattaa atcttttaca
    17341 tttaatgact tgccatactt tactgcctta ccagtttcat ctatcaaatc atatcccatc
    17401 tctaagaaat catctatttt acattcttct atagttaata ttctatttaa tttccttact
    17461 tgtgccatta tgctccagct ccttcaacaa caaattgtat tgcatcagct tttttattta
    17521 atataaatac atcctcaaaa ctttcttcaa agtagaagta ttttccctct gtaactgctg
    17581 ttggttcgtc taacttagag aactgataag aaacaggtgt aattattgca cttgggtgaa
    17641 ctaaggacat aaagatttgt ttagctcctg ctcctacttt ccatccagtt gtaaaatcat
    17701 atgcagtttt cattagatta gatggtactt taattatttt aactgtgtca atatcagttg
    17761 tttgacgatt aagagaagtt cctgcatcct ttatatttac tgttctttgt atctcttttg
    17821 catttttgat aagtgtattt actactggag taacatacaa tattcttcca ttttcaggta
    17881 ctctagcttc tgtcattttt tccattaact tatcaaatac ttctaatacg tttgttgttg
    17941 taagaacagt tgtatctgct gtgttaccta atgcggtcca atcagcatat attttagata
    18001 tacagtaagc atccatctct ggaaactttt gttcctcatt atatactttt gttatattgc
    18061 ctattgaagc cacataatta gtttggttta tatctgctgg atgaaccaat gttgaccatt
    18121 tcctttgatt agttaatacc ttaggttccc aagcattatc atagtttctt tgagctactg
    18181 ctattgtatc tctgtttgaa tctactcttc cagttgtaga tatagttggt atttctattg
    18241 ttttagaacc agtccatcta tatcttccat tatttggtgt tgcatacaaa tccccgaagt
    18301 ttaaagtata aggatatgct tgtgctaaaa catttgaata ttcttttgca taatttagtg
    18361 ctgccatttt atttcctcct atttattatt attttcatga ggtcttaccc cagtaaaatt
    18421 aaaaccaaaa tcatttatct taggctcttg ccctggtgtt atagtatcta ttttaggctc
    18481 ttcaccttct agtgttgcat taaacaaata atctttatcc tgtttcaaag ggtttatttg
    18541 ctcttcaaaa gctttttgtc tatctttact atttcttagt gcttctatat ctaaatgagc
    18601 ttttaacgct atttcatccc tacatttaat agacttaaaa gcatcattta accaataatt
    18661 aaagtccttt tcttcaattt cttttttgta ggtttcttcc aaagttttct tatcagtttc
    18721 ataagttgtt tttagattct ctacatcttc ttttgtcata cctccttcaa actttttaat
    18781 agcttcatta gctgtattaa gttgtgtttc aagatttgta taatcttctt gagtaattgt
    18841 agtctctttt attttctttt ctatagattt ttgaagagaa actacatcaa ttttgttatt
    18901 ttctattttt attccttcta gcaattcttt taaccaatcc atcttttaaa tatctccttt
    18961 cattttttac ataataaaag cacctactaa tttatcattt agcaagtgct tttacatatt
    19021 tactatttgt atatctttcc ataaatcctt tagtaattta ccatcaatgt tgtaattatc
    19081 aaccatatcc ttaccatttt tataatactt tgtatctcca ttagggcaaa gagtaataaa
    19141 ccttgtatca tcatctccaa tagatatatt atatggttta ttatataagt caaattctat
    19201 atctaatcct aaatcaatag aatcaattaa atgttgtaaa ttcttaaatt tattatccat
    19261 tttactctcc tttcaaaata tctttgttag ctattttatg agcttttgta agctccatat
    19321 ccttctctct ttttacctta tcatggttat tttcatcagc taaccaatca tgtttatgag
    19381 gtacaatttt atgttgcttt gggtttctat ggtcagttaa gtctaaatct aatctaggtt
    19441 ttcctgtatt accatagtat cttctttgaa ttaatttacc atctttgtaa ttatcaaata
    19501 cactatttgg ttttgattca aatggcactg aatgaacact tccactagtt aaatttctct
    19561 gattcttaac ttgccaattt acatccttat aaagcttttt agcttcctca taccttatag
    19621 tatcattata cttcatatgt tgatattcat caaatttaga aggtatttca tttcctaata
    19681 cctttttata ttcttcaaat tgttttctat ctttactctc atttagttgc atttttctaa
    19741 gggtatcagc tttttctttt ccaagtctac cctctatatg tttctcatac cactcattat
    19801 acttcatatt agatggtaca taatatgttt ttccatcttc tccttttgct gctctgtaac
    19861 cttcttcatc ttcaaaccaa ggagctgttg ttgtcctaca acgacaatga aatggtggag
    19921 ctgttattcc aacttgataa tctttcatat caaatacttt accatctaat tctctgcata
    19981 tatttgaagt tcttaaatct agtgtagcaa ttatctcata tttctctaca tctaaatcat
    20041 taaaacaatc ttttctactt gctgatgcaa agaaagctga ttcagtcatt attaagttct
    20101 tagcttgaga tttagataca ttaaatcttt tagcaaagtc atttactaga ttctttggat
    20161 tttcacctct aataattgat tgagttagct tagtatgtag ttcatttact aaagcatgtc
    20221 tatgcttacc ccaaattctt tcactaaagt ttaatccatc tgttgcccat ggtttagaga
    20281 taattttatt tattctatta gtatcaagac tcattaaact ccaaccaacg tttactcctt
    20341 gttgaacatt aaaagctgta tgatagtatc cacttgtata aatatctctc attagtttat
    20401 caataccatc aagttcattt ccatataaaa cttctacttg ttgctgtatt tgtaacttta
    20461 aagcttcaag ccttgttata tgaactcttg cactagcatt ttctaactct ttcatccact
    20521 tttgatttat agcattttct ttaccatatt taatatattc ttctacactc catttaaact
    20581 cttctagttc tcttgtattt agtagtttct tagcttctaa taaagatatt ccttcatttt
    20641 tggcaaatct gttgtaccat gctaatatat ctttttctat attatttata gctagtttat
    20701 attgcttttc taattcaaga taatatttta cacttttgtt attttgagct tcttctaatt
    20761 gttcaaatct ctttctccaa taatctttat gtttcatcta taacaccatc ttgattatta
    20821 ggaattaaat catcatactc tttttgagta tcttcctgtt ttttaagtct ctcaagttcg
    20881 tcattaacat cctcaaccca aggatgattg gaaacaatag tttcatctga tatgattcca
    20941 attgatttag ctgccatatc tattttttca gactcattta ttatcattga gtggttaaaa
    21001 gtaatttgaa ctgatttata atcataactt ttactaccac ttatctttaa atactcacat
    21061 acaaaccaca aaagttctct aattgctttt ttaaacttct tttcagtttt ggagcatttt
    21121 aaatcaagta gtgaatataa aaatttaagt gctacaccag atttgtcacc tgtgttttga
    21181 gattctggat taactccttg accaaagata attatattct tttccaatct atcaagaagc
    21241 tcctttttag cttcaactgg tatatttatc tctagtttat caactccacc tccaccatct
    21301 actttaattg atttatagta tcttatatta tctataaact cttgtagact tgttcctgga
    21361 tattctttta atacataaat aacctcttgt atttcatcta agttatctgc tagtgtagaa
    21421 atattattgt catatatatc tattaatgat ttatagaaat ttaaatctga tacgcacttt
    21481 tcattatttt taaaaggtat aaatggaact ttaccccatc cctgttcttt gttatttatt
    21541 ctaaaatgac cttcttgtat atcagtcatt tttccatatt catcatataa aaattcttga
    21601 ataaaactat tacctctttc aataaagtaa gttacgtcat tttctgtgta gtactcaact
    21661 ctttttattt tatttccatc tatatcttca atataataaa acctaataaa tgcaactaat
    21721 tccctctgtc ttttactatc ccaaatagga attgcttctt cagcaggtat tattacatat
    21781 ttaaactcac cttttctatt aatatatgga tgtaaccatt caataccttt gttactagca
    21841 ttgaggtaga gttctgttat tgtatcgtca aattcttctc ctagtaagtc atttaaaagc
    21901 ttagtgagat tatcatcatc tgcattaaat actatgggat ttccgacact atagcctacc
    21961 ttttggtcaa ctaaaagctt atggtagttg ttaattgctt tattattaac tttagtaaaa
    22021 tcatcaacct tagctccatc taagagataa tatcttctct tattgtttat atctgcattg
    22081 ccataatagt attcttcacc ttgtttatat ttctctggtc tatgttttaa aatataatgc
    22141 tctataactt ttactaggtt aaaggtgctc tcttttttta actgaacttt tattaaatct
    22201 gtttcactta tataaatatt taacgcctcc tttactttaa gaagcttatt ccattatttt
    22261 taagcttatt atctatagaa tatctaagcg cagccattgc atcatccata aactcaactg
    22321 gttcatcaag atataatcca gttctttcat cttgtttcca tttccattgt tgtatttctt
    22381 ttatggtatt agtgcaacta ggatgtacat gtattcttaa ttgtttcaaa taatctattt
    22441 gagctttaac acttcctggt ccttttttaa ctcctttagc ttcatatcct gcactcttcc
    22501 acatcttaat tctatctggt tcagcactat cacagtacat aaatagattc ttttctaaac
    22561 ctttattatt tgcaatcttt atgatttctg aggtatccat ttcatgtaca tatatttcgt
    22621 tacatatata taattctcca tccttaaagc caattctaag tactacattt gcatggttaa
    22681 atccaaagtc ttgtgataac ctcatattgt caaaatactc aaattctgta ggaaattcat
    22741 gtataacata atttttaagt attgctccac cagtttctcc ccattctcca agtccataga
    22801 ctttatatcc ttctgggtct tgctcttttc tcatttgcat tcttctatag taagcttcat
    22861 ctataaatct attttgtaga taagtactat gatgagtaaa tatatcatca tttttatagt
    22921 caaaatactt tctttttatc caatgggtag ctgagactgg attaaatgta aacgtcattt
    22981 gataatacaa gttaggatta gttaaaatac ctcttaaacg gtcgtctagt atgtctatat
    23041 cactttccat aagttctgta gcttcttcac accaaaccca tgttaatttt ccttttgaga
    23101 agttaattga ttttaatttt tctctttgct tagcatcatt aacccctctg aaaattatag
    23161 aattaccagt aattttactt ttgatttcta aaggatttaa agttgttttc caatacttat
    23221 cagcttgttt accataaata cgatttatag ctcctgtaag ttctgcatat gttgagtatt
    23281 tgtgcgtagc ttctgatttt ctaactacta atagattagc tccttgatac tttttatctc
    23341 ctaactttag tatataatcc tgtgctacat taacagattt tccactccct gctgaacctt
    23401 tcattgctct gtatcttttt ttagtaaaat tagcttcttt aaaagatgga ttaaaatcta
    23461 ttctaactct cattgctatc accataatct acacttattt tcaactcatc atccccaata
    23521 tcatctttac ttaggttatc aacctcacat tttaacttct caactctatt tttctgctcc
    23581 tctgtagcca aattccaatc cttatgaatc atttcatcat actgcttaat aagactcctt
    23641 aactcactca ttgccctact ctgtgcatta agaaaagatg cttgcctatc ccatgcaaat
    23701 tgaaattcat actctatctt ctcaccattt tctgtgcttt catgtttctt taattcctta
    23761 atcatttctt ccttgtcttt aacatacatt atcttctgtg ctcttattat tgctgcatat
    23821 tgtattgtta tctgttccca aagaatatca aatttatctt tattctttat ctcattaatt
    23881 aagtcttgag tttcttcagg taagtatttt gagaagaaac caaacttttc agcattttta
    23941 ttaccaggtg gaccagtagc gtttttatta cctatgggtg cacccctttt atttttaggt
    24001 gcacccttct ttttctcact agcccaattg tatcttttta tccatgactt taaagtgttt
    24061 aaactaatgt catactttgc tgatatttcc ttttgtttca taccttttat gtaatcttgt
    24121 tttacctttt ctttgacatc ttgcacatca ccacctcgtt tgtttgtcgt tttgggaatt
    24181 aaaaaagacc ctccatcaag acagtcattt aaatcatttc tattaattct ttaatctttt
    24241 tatatacttc tttataattc atatctttat ctattaactt aggtaatttc atagatataa
    24301 ttctttcaag tgcttgtata tcaaacagtt cactttgatt taactcatcc cttttcacac
    24361 cttttggtat acctaatttt tttcttacaa gttcggtaaa atgtttataa tacatctgag
    24421 gtttattact tccttgacta attgcataat aaacaaattc ttgtatttca tctgtaaaat
    24481 cttttcttac tttttttacc ttctgttctt atatccaacc attcttggtc tttttctgta
    24541 gcaatgtagt aaccatgtat tctaatttgt ttaagtgttt gtgtaaccca ttttgtaaat
    24601 aactttgctt ctggtttatt acttctaaat gacatattgt acacagcttc ttctgtaaca
    24661 aaagtagtac cgaagttagg taatttatct ttaaagtttc tagtgtagga atctccgaca
    24721 gtagactcat taaatttctt tttatattct ctatctatat ttcttaatgt atcacgaata
    24781 tttactatgc ctagttcctc tcctacgtca tttgcattaa accaaacttc ttctccattt
    24841 ttggaccaca ttacttttac atttttctct tgtaaaattt tcaacatact actacctcct
    24901 aaattattta tattaataaa gggtgctcaa tttgaacatc ctttttatat atcgacacct
    24961 caatttgacc ccccgtcatt tcataaaatc aacctcctgg gcatttgaat tttagatatg
    25021 catagtgtgc atatccgaat agtgcatatt atgcattttt aatagacctt ttcagatgtc
    25081 ggagttaccg acatctgaaa cttattaatt tttgtattaa aaaagaccta gaaattaatt
    25141 taagccttat ttacaccatc ttttgctttc atattcacta tataatttgc tgtaaacact
    25201 actcctctta tcaataactc ttcactaata gcacttttcc ctataaaact ctcaacatat
    25261 tcaactccat aacttaaaaa ttcatcatct gcatctatat taaattcatt tagttctttt
    25321 attatctttt ctcttaaatt actcatttat catactcctg taaaaaaata aaagactaag
    25381 cttaccctca cttagccttt ttaaataggg agatacatat attatgtcgc aagttctaag
    25441 aatcgaactt agattaagca ccagcacctg catggtgagt gaggttacca agccccaccc
    25501 gatttttaga cttctgaatt aagatacaaa atataaaatt ttgccctcaa tttctctact
    25561 tttagcgtat acgttgatta atatttgaac atagttagaa ttgaactaac aacgtcctca
    25621 cgccctgcct agtctgttca tattgctaga ttagcccttt aaactaacct agcaattatt
    25681 tagttttgag agggaaatct ttatttccac gatattatta tctcatgttt ttgccaacaa
    25741 aaagtctcac gatagtctca aaaaagtctc aaaatagtct catttttaag ctttccatga
    25801 aaaaatgggt aattcaaact cttttatttt tggatataac atatccataa ttttacaaac
    25861 tatccttttt cttattctaa agcaatgact tctatctatg tgcatagcat tagacatata
    25921 atccatgttg attttctcat tgttcatata catttcattg aagaactctg tttcaaagct
    25981 atttagactt gttaatgcac attctatagt ttctttttca atttctaatg ttttcttatc
    26041 ttcttttaat ctatttaaat cttcttctct cttaataact tcattttcaa cacttgaact
    26101 tatattataa gtttctccag ttttttcttc ataactttga gccttacatc cgcaaaactc
    26161 actttctaat tttttaatat atatatcttt tattcttatt tgactttcta gttttttata
    26221 gttatacaat ctaccttcaa cctcttgaaa tagtgtcttt ttattcatac ttccacactc
    26281 ctatcaatta tttatgttat aataatattt gtatataaaa gttttatatt tttgacaact
    26341 ggagtgtgaa agcactcctt ttttcttttt attaacagaa attatctttt tcaaagaaac
    26401 taatctgatt tgtctttctt tcagacttta taattctaat tgattcatct ataatgttta
    26461 gggattttaa taacacatct tttggtatgt cttcccactt atcagctcca agaattaata
    26521 aagttctctt tttaactaaa tcaaactcct cattagctct aggaatatct aatctatttt
    26581 ttatgtagct tgaaatatca tacttagttt tacttgtagg tctataatat tgtgaggatt
    26641 cctttttaaa ttgttctatt tgtgcatcat gtttggtttg catttcacat aatgcgttac
    26701 ttacaatatc attaatttga tttaactgtg tttttgtaag attgtttgat actttgtatt
    26761 tttcttctac tgcaattaga tattttctat aatttttaga gtttctttta agttctttat
    26821 ttgctcttgg acttgctcct gctactaaac aaatttcttt tgctatttct aatttaatta
    26881 tatattcgtc tgtaggtctt ccaccctgtt tagaggtttt accttttaaa atgtaaaact
    26941 catttttatt agcatccaca ttttttaagt ttgtttctat ccagtctgaa aattgttgtt
    27001 ttactcctaa tccttcccaa agttctcttc catttacaac ttttatatct tcatctgttg
    27061 tatatacttt tataagtcct tcattctcga ttaattttaa attattcata cttataatcc
    27121 tcctccaaat tatttttatc taatgatttg ataaatggat ttttccattg ttcaactaat
    27181 atcttctaat ataacctcaa ccctgggatt gtcactataa tatttactag ctacaacctc
    27241 aacaatctgt gtatcatcct tataagctat ctcattgagt gaatcagcta taatcttaac
    27301 aacattgtct atatcgggtt tcttactagg tcttaataca ttatttcttt tctgctcttt
    27361 aaccttttta ctgttacttt tagctataga atagtaacat cttaaagtca tttttatata
    27421 accagtaaaa cgatacttaa ctttagattg atacagccat tttattaact cttcataatt
    27481 cttagtctta ttaggtgtat aggtcctttt agttatagag ttcattctcg gtctttcttt
    27541 gccaactggc tctccatcta ttacaaaatt aactttcatt tgctacctca tttagttcta
    27601 tttcttccgt atctctaaca acaaaccaat atggtttata tccaaattca tctatccatt
    27661 ttttaaatac tttgcttatt ctattctcta gtgttaatat gtcttcagca cgtatatttt
    27721 caaaccaatc ttctccaaac cctccaaatt catcatcaat tctatcttga acacgttcta
    27781 aagcatcctc tgcatctata ttaggtatat taacatcttc tttttttcca atataaacac
    27841 attgtttaac ctctttaaac cttttaagtt cttccttagc tccattaata gcttcctctt
    27901 tgctttcata ttcatcactt gcaaaatatt catcatccca actatataac caaatatctt
    27961 tttgcatatt aatacctcca ctatttattt ttcttattag ccttctttct acagtcctta
    28021 caacaataaa tctccttaga tttttcctta agataaaaca gcttaccaca ccaactgcat
    28081 cttcttcgtt tcatagaatc acttcctagt cacaaaacaa atttataaaa ttctctacat
    28141 atctatattg ttgtttaata taagcatcat cttcattacc accagtagcc atccagccac
    28201 atattcttct atctatatca cttaacacgt caaatgggat attatactta tttaaagcat
    28261 catttaattg ttgtatatta tttatctcaa ccttattttt catatgcaaa tcaccctttt
    28321 tataagtcaa agtaagtcta taaacttcta gtttcattca caaacttacc ttgactattt
    28381 atactccatt tttattattt aatatttagg aaattctcca taagtcgtag caagacattc
    28441 aattctccac tctggtataa cccatgttgt attgttatca cctacatatg cttttacttt
    28501 ataaagcggg gtatttcttt ttctaaattt cgtactataa catttttcta aaatttcaca
    28561 accaaccatt ctgttatagt aacagtttat ttgatgatat ataatctttt cccctacaga
    28621 atatttatat tttaaattat ttatagattt tttatatatg cgatttttta cataatttgc
    28681 taatatacaa ataattatta gcagtatagt caataaaaaa atcttcataa gttattcctc
    28741 ctaactagtt taaatttaca tcctcttatt atttacttct ccttctctag ccaatagtca
    28801 catgagtacc tacttttcct cataaaatgt tacattctta ataattatat ctatagaccc
    28861 attttgattt tgtcttacag tatacttcat tgggtcttca aaatcagtta gattcccttt
    28921 tatgtcaaaa ccattatccg tttttatact tcttttttta agctttttct caacccattt
    28981 tttatctata ctaaaacttt cgtcaagacc tttttcttcc atatgctctt taaagctatc
    29041 ctttaactca tcatctttaa ttgaattatc aacaaagtca tttatatcaa cttcatgttt
    29101 ttccttcagt gtataattta atatacttct tacatcttca gcttgcttta tatcattact
    29161 aagagcattg gttatccaat tttcagctgt atttttaaat accttggtct tgtacttatc
    29221 atcatctatt tttttagcat ttaaaaattc tgttacaaac ttagattctg acccttcttt
    29281 ttcagcatcc ttatctaaaa gccttagatg aaactcatca tttataccac ttagactaat
    29341 caaagctcct tgcttttgtc ttaaagtctc tgggatacca atttcatttg aaaccatttg
    29401 aatattaaat ttatcatcca caaattcaat tgaatgagtg tacaactttt tataatctaa
    29461 tttcagtatt gctacactct tttcatcttt aatagtgtat aaacaaattg ccaaatcaca
    29521 agattctagt gcagcattaa ttttcataac gtcaaacaag taagatgcaa tttctttaga
    29581 attttt
//
LOCUS       DAYAOX010000019        29426 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.019, whole genome shotgun sequence.
ACCESSION   DAYAOX010000019 DAYAOX010000000
VERSION     DAYAOX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.019"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(142..429)
                     /locus_tag="ACGI57_000762"
     CDS             complement(142..429)
                     /locus_tag="ACGI57_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFG9715848.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(507..932)
                     /gene="ruvX"
                     /locus_tag="ACGI57_000763"
     CDS             complement(507..932)
                     /gene="ruvX"
                     /locus_tag="ACGI57_000763"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFG9715849.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(1138..3309)
                     /locus_tag="ACGI57_000764"
     CDS             complement(1138..3309)
                     /locus_tag="ACGI57_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715850.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDIMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            complement(3319..3576)
                     /locus_tag="ACGI57_000765"
     CDS             complement(3319..3576)
                     /locus_tag="ACGI57_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFG9715851.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(3601..6240)
                     /gene="alaS"
                     /locus_tag="ACGI57_000766"
     CDS             complement(3601..6240)
                     /gene="alaS"
                     /locus_tag="ACGI57_000766"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFG9715852.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(6308..6546)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6689..7768)
                     /gene="mnmA"
                     /locus_tag="ACGI57_000767"
     CDS             complement(6689..7768)
                     /gene="mnmA"
                     /locus_tag="ACGI57_000767"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFG9715853.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(8057..8497)
                     /gene="nifU"
                     /locus_tag="ACGI57_000768"
     CDS             complement(8057..8497)
                     /gene="nifU"
                     /locus_tag="ACGI57_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HFG9715854.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(8551..9744)
                     /gene="nifS"
                     /locus_tag="ACGI57_000769"
     CDS             complement(8551..9744)
                     /gene="nifS"
                     /locus_tag="ACGI57_000769"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HFG9715855.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(9762..10208)
                     /locus_tag="ACGI57_000770"
     CDS             complement(9762..10208)
                     /locus_tag="ACGI57_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFG9715856.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(10317..11087)
                     /locus_tag="ACGI57_000771"
     CDS             complement(10317..11087)
                     /locus_tag="ACGI57_000771"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9715857.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(11118..12383)
                     /locus_tag="ACGI57_000772"
     CDS             complement(11118..12383)
                     /locus_tag="ACGI57_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFG9715858.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(12490..13275)
                     /gene="codY"
                     /locus_tag="ACGI57_000773"
     CDS             complement(12490..13275)
                     /gene="codY"
                     /locus_tag="ACGI57_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFG9715859.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(13440..15527)
                     /gene="topA"
                     /locus_tag="ACGI57_000774"
     CDS             complement(13440..15527)
                     /gene="topA"
                     /locus_tag="ACGI57_000774"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFG9715860.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNIGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(15560..16660)
                     /gene="dprA"
                     /locus_tag="ACGI57_000775"
     CDS             complement(15560..16660)
                     /gene="dprA"
                     /locus_tag="ACGI57_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFG9715861.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(16679..18280)
                     /locus_tag="ACGI57_000776"
     CDS             complement(16679..18280)
                     /locus_tag="ACGI57_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFG9715862.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(18311..18655)
                     /locus_tag="ACGI57_000777"
     CDS             complement(18311..18655)
                     /locus_tag="ACGI57_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFG9715863.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            18839..20002
                     /locus_tag="ACGI57_000778"
     CDS             18839..20002
                     /locus_tag="ACGI57_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9715864.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNYTKTFNSIYFVPLILDISFLIKEKYK
                     YILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSIALYRLIQESLSNSSMHGKATEIRIFITFNTSNLIITISD
                     NGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            19995..20642
                     /locus_tag="ACGI57_000779"
     CDS             19995..20642
                     /locus_tag="ACGI57_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9715865.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTIKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            20771..21706
                     /locus_tag="ACGI57_000780"
     CDS             20771..21706
                     /locus_tag="ACGI57_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9715866.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            21720..22817
                     /locus_tag="ACGI57_000781"
     CDS             21720..22817
                     /locus_tag="ACGI57_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9715867.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGNLIFTCLIISTILIALAIVKEKYKWREV"
     gene            22820..23884
                     /locus_tag="ACGI57_000782"
     CDS             22820..23884
                     /locus_tag="ACGI57_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9715868.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            24128..24625
                     /locus_tag="ACGI57_000783"
     CDS             24128..24625
                     /locus_tag="ACGI57_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HFG9715869.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(24801..25049)
                     /locus_tag="ACGI57_000784"
     CDS             complement(24801..25049)
                     /locus_tag="ACGI57_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715870.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(25115..26131)
                     /locus_tag="ACGI57_000785"
     CDS             complement(25115..26131)
                     /locus_tag="ACGI57_000785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFG9715871.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCVNSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(26189..26956)
                     /locus_tag="ACGI57_000786"
     CDS             complement(26189..26956)
                     /locus_tag="ACGI57_000786"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFG9715872.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(27024..29213)
                     /locus_tag="ACGI57_000787"
     CDS             complement(27024..29213)
                     /locus_tag="ACGI57_000787"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HFG9715873.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYGISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKKFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEGDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         9332 a         5179 c         3115 g        11800 t
ORIGIN      
        1 ccaaaattca taaatttttt ggtattttta ataaaaaaca aaaaaacaga tatatactat
       61 aaatgctttt acaattaata atgtttaatt aaagcaaata taataaatat ctgattttta
      121 tatacaaact taattactta tctagttaag tccatttgat tcatcttcgt cgtaatttaa
      181 accttcttct tccattatag cagtatacac agctacaaca gtttcatact cttcatcact
      241 ttccaaaggt attaagattt caccttcttc atcctcatca attctaaaga ttaaagcttc
      301 ttcagcttct tcatcatcta aaggcattaa tattgcatac tcttttcctt ctgcttctaa
      361 tgttaatatt atttcaaatt ggctttcaac gccattttcg tctattaaat ttattatatt
      421 ttcttccata atgtcattca cctcaaattt atttttattt ttattattta tattatatat
      481 cataagtatt caattttata gttcttttat gaatttattt taaaatctaa atatccttgc
      541 aaaattatta ctgctgccaa catatctata accttttttc ttttttgtct actaacatct
      601 gcttctaata aagacctctc tgccgctact gtagaaagcc tctcatccca aaactctaca
      661 tcaatacctg tttctgcttt taccaattca caaaacttga ttactttttc tccttgtgga
      721 cctaaagttc cattcatatt ttttggtaat cctgatacaa ttttatttac ttgtttttct
      781 tttattattg cttttagctc ttcaatatct tttttcttgc caactctttt tattgttttt
      841 acaccttgtg cagttaatcc catcaaatca ctcacagcaa ctccaatagt tttatcacca
      901 acatcaagac ccattattct tccatctaac atagttcctc ctttatctat ttatattcta
      961 tagattaaat acatattctg aacctctcat aactaaggtc acaggcttac tgcttcatat
     1021 aattttacat aatcaatcat aaaaattgca atatgatttt ttatgtaagc atccagttta
     1081 cattaataat catattgcat tttttattta aatcctatat gaacagtaaa aattttttta
     1141 gttatttttt tcaaatatta tcatatttat acttattatt attagtggta taagtatata
     1201 tatagatgct gtagatgcag caactattcc agaatcatta actaaaagtg taattataca
     1261 accaaccata gatgcaataa accctttaaa caagattgga aaattattca ttatctttct
     1321 aaagtgtcta gatggtttaa atataagcac tgcaattact ccaattccta caagtaaaat
     1381 atttacccat acacttgtct gtgctagttg catattcatt tgaatttttc ttccaaatgt
     1441 ttgtaatatc tctcctggtc catttaggaa aattctctgt acaaacatac ctaaatgaga
     1501 ttcactacct gaaagtatat ctaaagctgc aaatgcaaaa acgacaacta cagctgaaac
     1561 agctaataat acagctttct taaaatctat tttaatgtca tatataagca ttataaacaa
     1621 caaatatgct acacattctg atatagctcc accaacattt gcacccattg aaggatatgc
     1681 actagttata agtattatta atgaaaaaat tacaacaagt aatttagata ccttcttata
     1741 atgtaataat actgctaagc caaatactgc acttgctata gttacacctt catattcatt
     1801 tcctacacca tagtacctag ctcctatgac agcatcataa ctcattatgt tattttgcat
     1861 taagtatgtt ccaaatacag aatctataac tatgactaaa atagtaagtg ttgcaaaaaa
     1921 tcccatgtgc tttatatcat tgtccttaaa taattttcta cctaacaagt ataatattag
     1981 cgtagttgca aatattccca aagatattga aattggagtt ttaaaattca tcaaaggagc
     2041 taacataaat gatagtggca ttattatgcc taattttata aactccttaa gtactttgaa
     2101 tacacgctct ttatgtggca tataatttct aaagaaaatc gcaaacatgg caattaccca
     2161 agaagcagat attacgccta caaagccatt tataatagtt gaccttattg aagatataga
     2221 cactatcctt tcataaagtg attttagata gctaatattg tcctctttat ttactaaagt
     2281 ataacttcta cctaccatct ctttattttc aagaccaaat gtacctagta tatctactcc
     2341 aacatctaaa ttggcaacaa ttccagcctt tcttgttgtg gcagattcca gtagaccttt
     2401 cccttctcca ctaaatttta ttattggaga aagtcttctc ttattcttat aatctaggtc
     2461 acttggaaat gcacttgcta tataaacagt gtcattttct ccaaccatag aaaatacttg
     2521 ttcaagatat ttatttatat tttttacaat atttttcttc attatatcat aagtattatc
     2581 atttagattt aatctataca tatctaatct atatgtatct ccaagttcta caaatatagc
     2641 atcactattt ttataggatt ttttagtttc atctagtaat ttatcataat ctgtagatat
     2701 tccaaatggc attttagaat ctttcttatt aatattatca atattacctg aatcaatcct
     2761 tccatattgg tccatagcta ctaaacataa gtttctgttt ttaactaatt gattattttc
     2821 aattatatca gcatttccaa ttacagatgt ctttaaacca ttttctgata aagtttttcc
     2881 taatgaccct aaatttgctc catattctcc atactcaaga ttttcattta tagatctatt
     2941 aatgcctaaa tcatttattt ctttagcttt ttgacctgtt gcgacttcaa aagctttagc
     3001 agtatcttgg tctacttttt gaaaattgat atagtcttca gtagttacac tagctcttgt
     3061 tcctgcaccc atacttgcat aagaccttct atcatcagta ccttgatccc ctcttatatt
     3121 catgagagct acatagcctc tcttttctat ctcatttttc aagattggaa tattcatcat
     3181 acttccaaga ttggtcctat tcatatcaat aaatatgacc ttgccttttt cttcagcaaa
     3241 accatagcta gcagttgata caaaaatcat aatgactatt gcaaacaacg acataatctt
     3301 ttttctcatt tatttgacct actttctatt cagatagtgc gtgattactt cttcaagtat
     3361 ctcatctctt tcaaattttt taattatagc tcttgcattt ttatgactag ttatatagct
     3421 agaatcacca gaaaggagat accctataat ttgatttata gggttatatc ctttttcaac
     3481 taatgcttta tatacaaaat caatagtatc tcctacactc atcctatctt ctggtattcc
     3541 ttcaaatttc atagtgtagt ctaaatcttt ttccaaacta aaaacctcct tgagtgaatg
     3601 ttatttaact tgtgtcttaa taacctcact tgcatagctt aaagcttggt caaccttaga
     3661 tacatcacta gctcctgctt gtgccatatt aggtcttcct ccaccttttc ctccagctat
     3721 ttttgcaact tctctaacaa tattacctga atgtattccc ttatctaata catctttagt
     3781 ggcagtaact acaaagttaa ctttatcatc tactacattt gcaagtacta ctacaccaga
     3841 accaagctta tctcttagat tatctgctgt ttctcttagt gtatccatat ccataccttc
     3901 aaatttatta gttatcaaat taactccatt tacttcaact ttagaatcaa aaatagaatc
     3961 aactgattgt aaactcatct tagctttcat atcatgcaat tctttagata agtttttatt
     4021 ttcttctaat aaagaactta ttctttgaat taaattatct tctttagatt ttaagtttac
     4081 acaaacttca gagataattc catctctttc ttttaggtat tcataaacag cttttcctgt
     4141 tattgcttct attcttctta cacctgctgc tactccacct tcagataata ttttgaacat
     4201 tcctacttga gaagtatttg tcaagtgagt tcctccacaa agttctgtag aataatctcc
     4261 catagaaact actcttacct catcaccata tttttcacca aatagtgctg tagctccttt
     4321 ttctttagct tcctttatat tcataatatc acattttatg tctaaagatg ataaaataac
     4381 attatttact ttttcttcta ttacttttaa ttcttcattt gatatcgctt caaagtgagt
     4441 aatatcaaat ctaagtcttt ctggagttac taaagaacca gcttgattaa catgttctcc
     4501 taatacttct cttaatgcct tatgaagtaa atgtgtagca ctgtgatttc ttgcagctga
     4561 cattctagtt tctctatcta cgagtgtctt cactttgtcc ccattactta ttcttcctga
     4621 ttttattata cctatatgtt ttatactatt attagcacct tttttagtat ttaatacttc
     4681 taaaactaag ttttcattag ttatcaatcc acaatctcca acttgtcctc caccttcagg
     4741 atagaaagta gtgttatcta gtactataga taccttatcg ccttcttctg cactttgaac
     4801 tagctcatca tctttaacta tagcttcaat agttccttca ccataaattt cactataccc
     4861 attaaatgta ctgtcaactg tagattctaa ttttgataga gggtcttcct tccaactttc
     4921 tccatccata tttcctctag catttctagc tctttctttt tgcttttcca tttcttcatt
     4981 aaaagcttct tcatctacag atagatgttc ttcttctaat atttctttag ttaagtctat
     5041 agggaagcca taagtatcat atagtttaaa agcttcttga ccacttagga cagtctttcc
     5101 ttcatttttt aatacttcta tataagagtt caatatttct gttccttggt ctatagtttc
     5161 attgaatttt tcttcttcta ttccaactac ttttttaata taactttctt tttcaactaa
     5221 aactggatat gctttatcat ttacttttat aacttcatct attagtttct gtaaaaataa
     5281 ttcttttact cctaataact taccatgacg agcagcacgt ctaaggagtc ttctaagaac
     5341 atatcctctt ccttcatttg atggaagcac tccatcacta actaagaatg ttacagctct
     5401 tatatggtca gttattattc ttattgatat atcatttttt ggattctctc catattttac
     5461 tcctgttaac ttctcaactg cttctagtat agatttaatt gtatcaactt caaagatagt
     5521 gtctacgcct tgcataatac atcccattct ttcaagaccc atacctgtat ctatattctt
     5581 gttttctaat agactataat ttccttcttc atctctatca aattgagtaa atacatggtt
     5641 ccaaaattct aaatacctat cacaatcaca gcctggttta cagtctggat tgtcacatcc
     5701 atattcttct cctctatcaa aatatatttc tgaacaagga ccacaaggac ctgtacctat
     5761 ttcccagaaa ttatcatctt ttccaagtct aaccattctt tcttctggga aattcatttc
     5821 ttttgcccat atatcataag aatcatcatc ttcttcatat acagtcaccc atatcttatc
     5881 ttctggtata tttagccatt ctgtaacaaa ttcccatccc cattttattg actctctttt
     5941 aaagtaatct ccaaatgaga aattacccat catttcaaag aatgtagcat gtcttgcagt
     6001 tatacctaca ttttctatat ctcctgttct tatacatttt tgagatgtac acattctcac
     6061 acttggaggt acttctacac ctgaaaagta gtttttcaaa ggtgccatac ctgagtttat
     6121 aagtaacaaa ctcttatcat tgtttggtac taaagaataa ctatttgcta cataatgacc
     6181 ttttgactca aaaaattcta aaaacttact tcttatttca tttaatccca tcttttccat
     6241 atcatgttcc tcctaataaa ttttattttt tcgtttaaat aaaaaacctc gtccctataa
     6301 ataaatttat aggggcgagg acaatcgcgg taccacccta attatcatac aaagtatgat
     6361 atcttcggaa aaccttagct ataacgtaac taccgtcaat ctctacttaa atcttcttta
     6421 taaaagataa attgttcaag attgctacta aaaggctgct tcaaatatca tattctaagg
     6481 gcttccagca tttccctctc tctgtaagac atcaaatatt ttactattcc ttatcactgt
     6541 atttgttttt taattaatat actaattata ttactaaatt ttacaaaatt aatcaatact
     6601 atattttcat atacaataaa tttattttat aaactttatt tactcttaat atactttatt
     6661 gcttattttt ttaatttttt agatgttttt ataaactttt ctcgattata cctccaccta
     6721 ctacaatatc tccatcatac attactacag attgaccttt agtaatagct ctttgagctt
     6781 catcaaaaac tattttaata gtatcttctc ctactctatg aatagtagct ggtgatggtt
     6841 ttgctgaata tcttattttg gcttgaactc ttaagggttc ttctaacgta tctattgata
     6901 taaagttaac atctttagca ataagttcct ttttaaataa atcttcattg tctccaagaa
     6961 ctatggtatt atttattgga tttatgtcta ttacaaacat tggttttcca aaagtaattc
     7021 caagcccttt tctttggcct atagtataat ataatatacc cttatgtttt ccaagtatat
     7081 tgcctttagt atctacaaaa aaaccttctt caatacgctt tttagaatgt ttttttacat
     7141 aatttgcata gtcattatct ttaacaaaac aaatttcttg actatcaggc ttattgtgta
     7201 ctgccatacc catttcctta gcaatttctc ttactctatc tttttcatag tcacctattg
     7261 gcaataaagt atgctctagt tgctcttgtg taagattata aagtgcataa gtttggtctt
     7321 ttttatctgt aacagatttt tttagcaaat atcttcctgt actttcatcc ttttctatct
     7381 ttgcatagtg acctgtagct acataatcac aacctatttg tctagccttc ttataaaaat
     7441 catcaaattt tatatgttta ttacaagcta tacagggatt aggagttttt ccttccaaat
     7501 attcatctat aaaataatct attacttttt ctttaaacac ttctctaaaa tttaaaacat
     7561 aaaatggtat acctatttta tttgctaccc ttctagcatc ttcaacagca gataaagagc
     7621 aacaaccacc ttcaatttca acaacatcat catcttgcca caacttcata gtaacaccaa
     7681 tgacatcata tccttgttgt tttaaaagat atgcagctac agagctatca actcctccac
     7741 tcatacctat cattactttt ttattcataa acaaatttca cctcatcttt tgcattactt
     7801 attattatac catatagagc aagtattgta cttatttaaa atatagcttg ttctattttc
     7861 taaaaaaata atcatcttta ttatttgaat cttatggatt attttgttaa ctaattaacc
     7921 tctaaatcaa gttaaaatac ttaatttagg caataaaaaa tagctatttt aaaaacagct
     7981 attttttatg tcctaattat atcattagta cttcttttta atcattgtaa acttctcata
     8041 acacaatcac ttatttttat tcttcttctt caacatcatg gtcatgagca tcatctataa
     8101 catatccatc caattctggt atatggatat tgttcttttg agcataatct ataagagctg
     8161 cttttacagc ttgttcagct aaaactgaac aatgcatctt tactggtggt aaaccatcca
     8221 aagcttctgc aacagcttta tttgtaagtt ctaatgcatc ttttatagtt tttcctttta
     8281 tcatttcagt tgccatagaa gaacttgcta tagctgaacc acagccaaat gttttaaact
     8341 taacatcctt tattacatct ccatctatgt ctaaaaatat cttcattata tctccacatg
     8401 tagggtttcc aacttcacct acaccacttg cattatcaat ttctcccata tttcttggat
     8461 tcataaaatg ttccataact ttatcactat attgcattat cttttcctcc tactcaattt
     8521 atgtctattt tttatgcttt atctttatgt ctattttact aaaccttgtt tcttcgcatc
     8581 atcataaagt ggtgacatgc ttctaagtct ttctattact tttggtaagt tctctagtat
     8641 ataatctata tcatcatctg ttgtaaaatc tccaactgta agtcttaatg aaccatgtgc
     8701 tatttcatgt ggtaaaccta ttgcaagaag tacatgtgaa gggtctaatg aaccagaagt
     8761 acatgcagaa ccacttgaac cagcaattcc taacatatct aataataaaa gtattccttc
     8821 accttctata aattggaatg caaagtttgc atttcctggc aatctatttt ctaagctacc
     8881 atttactctt gtgtaaggta ttctttctaa cacaccatct attaacttat ttctaagacg
     8941 agttaaagtt tctacatgat tttgcatatt ttccttagct aattctgctg cttttccata
     9001 tccaactatt gcaggtatat tttctgtacc agctcttttg cttttttctt gtgctccacc
     9061 atggacaaag ttatgaagtc ttacaccttt tcttatgtat aaagctccta caccttttgg
     9121 tccatatatt ttatgtgaag acatactcat caaatctatt cctaattctt taacatctac
     9181 tggaatatta cctgtagctt gtactgcatc agtgtggaat aatatcttat gttttttagc
     9241 tatttcggat atttctttaa taggctgtat agttcctatt tcattatttg caaacattat
     9301 acttataagt atagtagtat cttttattga atcttctagt tgctttaagt ctacttttcc
     9361 ttcgctatca acatctaaat aagttatttc aaatccatga tgttttgcta agtattcaca
     9421 tgtatgaagt attgcatggt gctctatttt tgaagttatt atatgattac ctttgttttt
     9481 attagcataa gctacaccct ctagtgtcca gttatcactt tctgaaccac ctgctgtaaa
     9541 atatatttca ctaggttcag catttataag ggctgctact tgttctcttg ctttatccaa
     9601 agcatctttt gcttctcttc cgaatgtgtg aaaactagat gcattaccaa aataatctgt
     9661 taggtatggc atcattgcat ccaatacttc ttttttaata ggtgttgttg cagaataatc
     9721 catatacaat cttctttttt ccattacatt catctccaaa tctatttttt aatatttgtt
     9781 atatcatttt ctaatttatt tttgttatag tcatttatca tatctttaag agtaattgaa
     9841 tctataacat cttctatacc ctttttcatc ttttcccaaa ctatttttgt tacacacatg
     9901 tttgagtttt cacatacatc ttcatctagt acacagtcag ataatgcaac tggtccttct
     9961 aatactacaa gtatatctcc tactgttatg tcttcagcat tctttgaaag caaataacct
    10021 ccttgagcac ctctcacact ttttactaga cccgattttt ttaaagatga aaatatttgt
    10081 tctaagtact gatctgatat tttttgcttc ttagcaataa attttaatga tactggtcca
    10141 ttgtcttgat ttaatgctaa ttcaaacatt gcttttagtc catatctacc tttagtagac
    10201 aacttcatat attatcacct ctatatagaa atctaattca tactgtttta gtaggaatta
    10261 ctttcaagat aattttatta taccctacta attttgtcaa gattaatttt aattttttat
    10321 tttgaataaa ttccccattt tccaatgttt tcaaatccta gttttttata tatctttcca
    10381 gcctcttcat tatcataaaa caaacaagga attttgtttt ctctcagtaa ctctgaacat
    10441 agtttaatta ggcattttgt agcaagtcct ttagctctat atttaggatg tgttcctact
    10501 ccaattatca ttgcatgagt cctattttct gaagtactct ttgccattga caccacctgt
    10561 ttgttaatct ctatacaata tcctctacct gtccttagtc cattttttaa attctctaaa
    10621 gttgtactgt gaaattcatc tattaagtca taaagtttta ctgtcttttt tagatttcca
    10681 agcctaattc tcttaacctt tgcattacaa ttattatcta tcaaaaactt tttagtttga
    10741 agtttgcaaa aatcaactac ctttaaatta ttaagtccta atctctttgc taaagcatct
    10801 acagcacata ttttcccaga tatttcttcg taattagtat tacgaagtag attataaaaa
    10861 ccttcaacat catactctcc atcagaataa aacatcataa attcaaaata tttaagtaat
    10921 attgccttta tttcaccatg ttcacccaca tctgcccata tatttaaaaa attatttcca
    10981 tatccatatc tttcaatatc ccctattata aacaaattgt actcaggttc tttttttaag
    11041 tatttcataa ctctactgtg ataagttgag ttaagtttta taatcataat atctaccccc
    11101 gatacataaa gttcagtcta aaaatcacta tttagatatt ctaaatattt ttttatattt
    11161 ttctcagttt tattttcttg tggtatgtaa taaatactat cttttatgct atctggtaaa
    11221 tactgttgct ttacataatg atttttaaaa ttatgaggat atttgtatcc acaaccccta
    11281 cctatttctt tagaaccaga atagtgagca tcctttatac tatttggtat atccttaaca
    11341 aactctttat ccagttcatc catagctttc attattgcta tacatgcaga attagacttt
    11401 ggagatgtag ccaaaagaat agtagcttct gctaatggta ttttcgcttc tggaaatcca
    11461 agttgcatag ctgagtctac acatgccttt acaattacaa tagcatttgg ataagctaaa
    11521 cctatatctt cactagctat tactaaaagt ctcctacata tacttatcaa atccccacct
    11581 tttataagtc ttgctagata gtgtatagat gcctgtgggt cacttcctct tatagatttt
    11641 tgaaatgcac ttaaaatatc ataatgacta tcaccatttt tgtcatagtt tataatttta
    11701 ttaaatgtag atgcccttat tacatctgaa tctatataag ttatattatc cttatttggt
    11761 ttagttgaat agactaccac ttccaaaata tttaaggctc ttctcatatc tccatcagaa
    11821 agaattgcta tatcttcaat agcatcatta ttacactcaa tatccatata gctatcttca
    11881 tttaatactt caacagctct tttcagtcct ttttctatat caagtttttc taaaggttta
    11941 aattcaaata cagtagacct acttaaaaga gctttatata cataatgata tggattttct
    12001 gtagtgctag caatcaaagt tatactccca ttttccataa attctaatat tgattgttgc
    12061 tgttttttat taaaactttg tatttcatct atatataata ggactccatt tacattattt
    12121 atacttccaa gctctgatat cacctttttt atatcttcta aagatgaatt agtagcattt
    12181 attttataga agcttttatt gcttctttct gctattattt ccgctactgt agttttacca
    12241 acaccaggag gaccaaaaaa tatcatattt ggtaaaaaat tagattgtaa tatcttagac
    12301 aatatttttc cattacctat tatatgactt tgacctatta catcattcat tgtttttggt
    12361 cttattttat ctgctaaagg cattttctca ctccttttta atatacttat cattacctta
    12421 ataattcgtc taaaaaaggt atctatttga aatagatacc ttttctaatc taaacctata
    12481 aaatatagat tattgattgt tttttaattt ttttaattca tctgtaagtt tgtcattaag
    12541 tattcttata tgagtacctt tcatacctaa tgatcttgat tctatcacac ctgcactctc
    12601 aaattttcta agtgcattta ctattacaga cctagttata ccaactctat cagctatctt
    12661 acttgctaca agtaaacctt cttttccatc caattcagca aaaatatgtt caactgcttc
    12721 aagctcggag taggacagag tgcctattgc catttgaact acagctttct ttctcatttc
    12781 ttcttccaat tcctcaccta ttgctcttaa tatttcaaga ccaacaacag ttgcactgta
    12841 ttcagctatt actaaatcat catcattgaa tgaatttgaa tatcttgaaa gtaccaatgt
    12901 tcctaatctt tgaccgcttc ctaatattgg aactactgta gtatattttt gtaatcttcc
    12961 atgttcttca ggaaagattt ctaatatctt ctcaccattt aaattttcta atgtttcatc
    13021 aatttttaac acattttgag tatattcatc tgaaaatttc ttttgcttag tatactcatc
    13081 ttctataact gaactgtctt gaacatcatt taaatgaaga cctagtactt taccttttgc
    13141 acttactaca taaacattag aacttaaaac gtcgcccaat gctccggcca gtaaatcaaa
    13201 agagacactg cttccaccac ttgtttgtaa tgttttattt attttccttg ttttttgtaa
    13261 cacttcactt gccatttaat catctcctca tgaaatttag gccttttaat aaccttttat
    13321 attttctaat attattttca catttaatga aaatttattt ttctattagt tgtttacact
    13381 tgaaagatta tcatagattg gaaccttttt caagagaatt atgtgatttt tttcaatttt
    13441 cactcttttc cgtcattatg ctcttttgtc tctttattac attctttatt agaacacact
    13501 gtttttgttt cagatttagt cactttttca accatatatg aaccacactt ctcacaaaac
    13561 tctcctgttg gcttattcca agctacaaag tcacagtttg gataattaga acacccataa
    13621 aaagctttac ctcttttaga ttttctaagt ataatatctc cttcttcaca ttttggacat
    13681 ttaacaccaa ttttattcat aattggtcta gttgttttac aatcgggata gtttttacaa
    13741 gccataaact ttccaaatct accatatttg ataaccatat tagaaccaca gttctcgcaa
    13801 atctcatcag tttcttcatc catatttact ttttctatat tttcaagagc ttcttcaata
    13861 gcctctttaa gtggtgcata tgattctgat acaatctttt tccaatctgt attgccttct
    13921 tctataacat caagtttact ttccatatca gcagtaaaat ctacatctat aaatttatgg
    13981 aaattatttt ccaaaatatc agtaactatt attccaagtt ctgttggaca tagacttgtt
    14041 ccttgctttt caacatactc tctattcagt atagtagcta ttgttggagc atacgtactt
    14101 ggtctaccta tacctagttc ttctagtgtt tttactaaac tagcttctgt atatcttgct
    14161 ggtggttgtg taaaatgttg gtctggcaat atattttcta tatttaatat atctcctttt
    14221 tctatacttg gtaaaatttt gtcttctctt tctgaaaaat tatatacctt ggtataacca
    14281 tcaaatttca tttttgaacc agttgcctta aatacaatat cgcctatttt acattctaca
    14341 tttagtatat caaacactga atcttccatt tgactagcta caaatcttct ccaaattaaa
    14401 ttgtataact tgtactggtc tttgctcaat gaggctttta tactctcagg agttctatca
    14461 actgaggttg gtcttattgc ctcatgggca tcttgaacct ttttagtatc ctttttttct
    14521 tttttatcac caatgttttt atagtagttt tctcctaaat tgtttacgat ataaccctta
    14581 gctttttctt ttgcttcatc agatattctt ttagagtcag ttcttatata tgatataaga
    14641 ccaacagtac cttcaccttc tatgtcgata ccttcatata attcttgtgc tatcatcatt
    14701 gtttttttag tagtaaaaaa taacttattt gctccttctt gttgaagcat acttgttgta
    14761 aatggttttg gagctgattt acgtcttgat ttagattcta tcttctcaac ctttaaatct
    14821 ttaccttcca tttcttttag tatttcattt acagcttctt cattttctaa ttcaatcttt
    14881 tcatcatttt taccataaaa tttcaaagtt atatctttgt cttttactgt cttagcctca
    14941 acatctattg tccagtattc ttttggaata aatgccttta tttctttttc tctatcacaa
    15001 attaactttg ttgtaacaga ttgcactcta cctgcactaa gaccttttct aaccttttgc
    15061 cataatatag gacttatttg ataacctaaa agtctgtcta gcactcttct tgcctgttgt
    15121 gcatcaacca aatttatatc aatatttcta ggatgtttaa ttgctttttt aattgcatcc
    15181 ttagttatct cattaaactc tattctacaa tcctcagatt catctaaatt tagtatatga
    15241 gctaaatgcc atgatatagc ttcaccttct ctatcagggt cagttgctag atatacttgt
    15301 ttagattttt tagcttcttt ttttaattct tttattacat cgccttttcc tcttatattt
    15361 atatattgag gttcaaaatt attttctata tctacaccta atttactttt aggtaaatct
    15421 ctgacatgac caactgatgc tttaactgtg taatgacttt tccctaaaaa tttttctata
    15481 gttttagctt ttgcaggcga ttctacgatg actaatgttt tggccataaa accatacacc
    15541 cccactacct aatcttaatt tatacattta aactgtacgt tttattattc atttctacta
    15601 ctaactcatt tagtactaat tcattgatta ttgaatttac atactttatt tctataccag
    15661 tataatcaca aatactatct atatgcaagt ttccttctct ttttatagct tcaattattt
    15721 gcttagactt ggcatttaga cttatattat catatttttg atttattttt ttatcaataa
    15781 tattaaatat gttatattca tttaaaatat catctatatt ttcaattaat tttgcacctt
    15841 cttttataat tttatgacaa cctctactca tttgagaatt tatattacca ggtattgcaa
    15901 aaacattttt tccatgttca agagcaaaat ctactgtgat tagtgcacca ctttttattg
    15961 ctgcttctac aacaactact ccatcactta aaccactgat aattctattt ctattagaaa
    16021 aatggtacgg ggctactgtc gagttaatat tatactctga taataataat cccccatttt
    16081 ctaatatttt atttgataaa tgtaaatttt gttttggaag aggattgtct actcctgaac
    16141 caagtactgc tatagtttta gattttccac tcatacaacc tatgtgcgag tatgcatcta
    16201 ttcctatagc taatccactt actatattaa ctccatattg ggatagttga caacttattg
    16261 tttttgcaca attaattcca taggctgtag gttttcttga acctactata gctatattaa
    16321 aattattatt aactaagcct atgtcacctt tgtagaataa taactttgga gcgttgtata
    16381 tattctttaa attttcagga tattctttat catatttaca aatatattta atcgcttttt
    16441 tgtaaagtag ttcttttata ttttctaaat aagcgtgacc tctatatttt actatatttt
    16501 ttctaatatt caagcttata ttttttaaat tatatatttc tttttcagaa aaatcaaata
    16561 tatcttcaat atttactatt tcattttcta ttatttcaat tgtttttgtt gttattccac
    16621 caattgattt taaccataaa taagtatctc ttttttccat aaaatccacc tcaaaaaact
    16681 atttaaaata actataataa gcctttctaa atgaaaaagc ttctataata cactgtgaat
    16741 ttatcaaatc tctttcttct aaatctgcta ttgttcttgc catttttaaa agttttgtat
    16801 aacttctact gcttaggttg tatttactaa atatcatctc agccgttttt gaagcttctt
    16861 tttccagttt acaatattta aataaattgt aagctttaat ttcactatta ttttttatat
    16921 tatctttctt aaatctattt atctgtattt ttctagcatt ttctactctt ctctttattt
    16981 tttgagagct ttcttcttgt tttaaagaat taaaatctga atattttata gaattaactt
    17041 ctacaaaaat atcaaaccta tctaacaatg gacctgatat tttatttatg tatctatcaa
    17101 tttcgttact tctacatcta cactctgtct ctgacatata ataaccacat ggacatggat
    17161 tcatagctgc aactaacagt actctacaag gatattttgc actgtacttt actctggata
    17221 tatttacata tccatcttct ataggttgtc ttaaagtctc aagtattttt ctatcaaatt
    17281 cggctatctc atctaaaaat aagatacctc tatgtgctaa agcaatttct ccaggtcttg
    17341 catccattcc tcctccaatt aaagattgct ttgtagttgt atgatgaggt gctctaaaag
    17401 gtcttttatc aattattcct ttagtttcat ttatcatacc caatatacta tagaccttac
    17461 ttacttctat catttcttct acacttatat ctggtaaaat tgtccttact ctttttgcaa
    17521 tcatagtttt tcctgagcct ggaggtccaa tcataaacat attatgattt ccagcagcag
    17581 caatttctgc actcctcttt acaaaataat ttccttttac atctttaaag tcttcatcat
    17641 atttactttc ttcattatta atcttagtat atttgcaatc tatataacta ttatcttttt
    17701 ctaattcttc gttctctttt cttgacttat catccaaaaa agacattatc cttactttat
    17761 cgacttttat ctcttcgttt aaaaaatcga cacattcttt taaactttta ataggaatta
    17821 tatcaatttc atctacaaat agactttctt ttatgttctc aattggtata aaaatacgtt
    17881 ttatgttctg agtttttgca cccataatta tgggcaatat acctcttact tttcttattt
    17941 tcccatctaa tgataattct cctataaaca tactttctct tatgtaattt tcatcttttt
    18001 ttattaactc tcttaaaagg cctatactta tagataaatc taaaaaagct ccttcttttt
    18061 ttatatctgc tggtgataaa tttacaacta ttctcgtgct tggaaattta tatccactat
    18121 taagaattgc tgactttact ctttctcttg cttctttaat ttctttgcct ggaagaccaa
    18181 caatattaaa actaggtatt ccattgctta catctacttc aaccttaact aaaaagctgt
    18241 cgattcctac taagttgctt gaattaataa tacttaacat atttaatcct ttccaaaaac
    18301 attatctttt ttaaaaagca ttcattatgt gatttattct tttttctaat aaatatacct
    18361 ctataacatc aaatcttatg ggagtgttat ttaagttatt ttttataata taataatttg
    18421 caactccaat tatctttttt ctttttttga agcttacaga ttcacaaggg tagccaaact
    18481 ttatgttaga ccttgatttc acctccacaa atataatttc atcttccatt tttgctatta
    18541 tatctatttc ccctatcttt aatctgtaat ttttttctag tatctttgct ccttttgata
    18601 gcaaataatt aactgctact ttttctccaa aatcaccttt ttctttatta ttcattattc
    18661 actccaatac attaaatttt cacttttaaa ttatggtcag tatacaattt tactattcaa
    18721 gtttttggat tgattttatt tccatcaata ttaataaata cttctattga ctttattctt
    18781 ttatgatatt attctagttt tcataggaaa agtttatttt tttggaggag tgcctttaat
    18841 gaacagagaa aaattagttg cttttatacg atatttatct ttatttatat tagttgtaag
    18901 ctatttgaaa aatgttgaaa atacattgta tccggtcata actatgataa tattatttct
    18961 aattataata aataatcaag ttagattttt ttctttatca aatgagaata aatttatact
    19021 tatttcttac tttttagaac ttatattaat ttttatactt tgtaattata caaaaacttt
    19081 taattcgatt tactttgttc ctttaatatt agatatatca tttttaataa aggaaaaata
    19141 taagtacata ttgttctctt ttgttataat atcttctctt attattagtt tagataaaaa
    19201 tatatatttg gcactggagt caagctctat attgctaatt ataactgtac tttctatata
    19261 tatagaaaat gagaatttat caaaattata cagtcaaaat ctttatgata agcttagaat
    19321 ttctaaagat gaattaaaaa aagttaatgc tgatttggaa acttatgcaa gttctataga
    19381 agaattagca attttaaaag aaagaaatcg aatatcaaga gaaatacatg atagtgtagg
    19441 acattctctt tctacaacaa taatacaatt aaatgctata gaaaaactct taaaagacaa
    19501 acctctaatt tatgacttag tacatgaact tagagaattt gtaactgaaa gttttcaaga
    19561 tgtaagaagg gctatatctg agttaaaacc tgttgaatat gaaaattacc aaagtttatt
    19621 taaaatcaaa gaattagtta aaagctttat taagcttaca aatattaatg ttaaattaac
    19681 tatttcaaaa aatacttgga acttatccag aaatcaatct atagctttat atagacttat
    19741 acaggaatcc ctttccaact catcgatgca tggaaaggca actgaaatca gaatttttat
    19801 tacattcaat acttcaaatc ttataattac tattagtgat aatggtatag gatgtggcaa
    19861 tataaaaaaa ggaaatggac ttaatagtat caatgagaga atatatgaat taaatggtaa
    19921 agttgaattt gataattcta ataatggttt cacaattaga gcttcttttc ctaaattttc
    19981 agggggtgat ttttttgaat aaaattaaag ttcttatagt agatgacgaa aaattaatta
    20041 gaaaaggtct taaaattata ttatcatctt ataatgattt agaaattgta ggagatgctt
    20101 ctaatggata tgaagcttta gaattttgta aaatgaatga tgtagacata gttcttatgg
    20161 atatacgaat gaaagtatgt gatggggttc taggtacaag gcttataaag gaatataata
    20221 attctataac attattaatt ttaacaactt ttaatgatga tgaatatata aaagatgcta
    20281 tgaagtttgg tgcttcagga taccttttaa aagatagctc tgatgaagta ttgcatgaag
    20341 ggattcgttc ttcatttttt ggcaatattg tgcttgataa aagtgtagct gaaaaaatca
    20401 tgacctctga aaaaacgatt aaacaagagc atctttatga catgtacaat ttaacagaaa
    20461 aagaaatatc tataattaga ctaattgcaa atggacttaa taataaagaa ataagtcaag
    20521 aactattctt atctgaaggt acaataaaga ataacataac taatatttta gctaaactag
    20581 aacttcgtga cagaactcaa ctagcaatct ttgctttcaa aaataaaatt gtaatagaat
    20641 aaatctaaaa atataaagtg acttaagtca ctttttttag tgacttatag taatagaatt
    20701 ataattcaca attctttatt ataatattat agctttttaa cttatacaaa attattttgg
    20761 agggattact atggatatag tcagagtaaa taatataaca aaaagattta atgataaatt
    20821 agtacttgat aacatcagct tttctgttaa aaaaggtgag atatttggac ttattggccc
    20881 aaatggtgca ggtaaatcta cacttataaa tataattaca aatcttatgt taccaaatag
    20941 tggtagtatt caaatcaatg gtttagattt atcaaaagat tatataaaag caaagtctat
    21001 aatagggtta gttccacaag aattagcaat tatagaaact cttacccctt ttgataactt
    21061 agagtacttt ggagctttct acggtttaaa gggaaaacta cttaaagaaa gaatcattga
    21121 agcattagaa gttacaggtc ttactgaagt caaaaagaaa aaggtcaaaa aattatctgg
    21181 tggtatgcaa aggagattaa atattggaat agcactctta aatcatccta aaatattaat
    21241 attggatgaa ccaactgtag gtgtagaccc ccaatcaaga aatcatattt tcaattttat
    21301 aaaagatata tctaaaaaac atgaaactac tgtaatatat acttcccatt atatggaaga
    21361 agttgagcac ctttgttcta aaatttttat tatggatgaa ggtaaagaaa ttgcttttgg
    21421 aaataatgat tatttaaaat cactcgtatc tacaaataca aaattaatta tggaaataaa
    21481 aaatataaat gcccaactta tctttgattt aaaaaatact aaaggtgtta tatctgtttt
    21541 agaaaataat tcattattag aattggatat agataaaaaa ttacaactta cagatatact
    21601 ctctgtcatt gacaaaaaca attcaaaaat aatgaaaatt tcttatgaag aacctagcct
    21661 agaagacgta tttttaaatc taacaggaaa aaatttaagg gactaatgga ggtgatttta
    21721 tgaaattata catgagtgta aaaactatgc taaagagttt aaaaagctcc tttatactta
    21781 atttaatata ttttttagct ttgcctctga ttttatcttg gtttcttggg atggttacag
    21841 aaagcatgtt tcaaaaccct ataaaaacag aatctacccc cattgttata tatgacaaag
    21901 ataacacaag attatcaaat gatttgacaa agtatttgaa aaatgattta tcttatatat
    21961 taacagttaa aaaagatgat tctaaggcag aattaaagct tactatacct aaaggctatg
    22021 agagtagttt attaaatgaa aaatcaaata ctctaaacat agaaaaactt ggaactcgtg
    22081 atgatattgc aatattgctt caagatatat tagatactta ccatgaaaaa ttttatttaa
    22141 ataattctca gaaaataagt tcagaagatt tttctaaatt atttaataaa aattctattg
    22201 atacttctat aataaataac aatgttaaac agagctctta tgaatacttt gctttagttt
    22261 cactaggatt tcttgtaata atttttataa tgaacaatat tttgtcaaat tatataagtg
    22321 aatcaaaggg actttcaaaa agactttatt ctatgcctat aactagagta caattcttaa
    22381 tttatgactt tgtaggttta tggatttatt catttatatt cttattatta tatgttttat
    22441 tctttagaat acttggaata acatttaaag gtaattttgc catattgctc ctactttgtg
    22501 ctttatcatc ttattttatg acttcaatat ctacctttgt aaccagcttt tttagtaaaa
    22561 aatacggtac agcaatagtt tatgcattac tctttttaca gacaattttt ggaggtatct
    22621 tcagtatgat aagtgataca tttactaagc ttacaagttt atctcctacc tatttaatag
    22681 gagaattgtt tagtaattat gaaacattta aaaccataga ttcaatagga aatttaattt
    22741 tcacatgttt aattatctct acaatattaa ttgcacttgc tattgtaaag gaaaaatata
    22801 aatggaggga ggtttaataa tgattagatt aactattgca aatataaaaa gatatctaaa
    22861 aaatcatact cttttaatta atatggttat gcttcctatt atactaattt tttcacttaa
    22921 tttttttatc aacaactcgg gaaatcaatc aatgtacttt tctccagtag caattgtatc
    22981 tgactcttca ggaaagtatg aacataagtt aataaatagt tctaaattaa aagaaaattc
    23041 atttagatta aatgaacaag ataaggctat gaatctatta aaaaataata aggtttctgc
    23101 tgtattcgtt ttagataaaa atttttcaaa tagtatagat aaattacaac gacctgtagt
    23161 taaatgcttt aaaattgaaa atggtggtgg tagtctttgg gcagaatcac aaatagaatc
    23221 ttttattact aaatcgttaa aattgaaagt ggataaaaat atagatgata aattgacaaa
    23281 gacaaatata attgataaca aaccagataa gaataaaggt tcatttttag tagtcttctt
    23341 gatatgctat tttatgtata ttaatgcggc acatcttgct tctgatttat ttacattaaa
    23401 aaaatcaaat gtgctaaaaa gacttatctc tacagataat aaagatataa aaataatttt
    23461 cagtatattt ttaggactat tttttattca atcaattgta tacacttttg ctctattatg
    23521 tttttcaatt atagaagact ttaatttgag tctgaatgta ttgatgattg tactagcaaa
    23581 tagttttgtt tccactggat ttgtattttg ggtagctaga gtatttaaaa atgaaagttc
    23641 tataagtctt atatctacat tttattcttt aataggtcta gctatatcta tctctagtct
    23701 aataccatca atggataaat tatcctttat gactaatctt tcaaagttca caccatttta
    23761 ttggactata gatgctatta aaaacaatgg tagtatttta ataaatataa tagctctaat
    23821 attaattgga attatatttg taactgcagg aagtcttaaa ctaagagatt ttgctaaaaa
    23881 ctaataatta ttaaatataa ttagtatcta tttttatata attgtaaaag aatatttcaa
    23941 catattaaat tattttatct tatatttttc tctttagttt cacacatcac caagtctaaa
    24001 ctctatatta tatattaata tttttgactt gtcaagttat ttatgttata atattaatca
    24061 ttagataatt aaagcattgt acctaagttg caatatctaa aattcaattt ggaggtataa
    24121 ctagcatatg tataaaattg gtgaaagtgt aatgtatcca aaagagggag cctgctctgt
    24181 aaatgatatt gtaacaaaga aaataaatca tgagatgcaa aaatactatg aattaagtgt
    24241 catttttaat agtaacctta aaatttcgat tccagttcta aatgcagata gaattggcat
    24301 acgtccagtt atggatggaa atgatgttga taattttatc caatctatta ataaaacaga
    24361 tggtgtatgg atttttgata gaaaagaaag acttaaactg taccaagata aatttcattc
    24421 aggagatgtt tttgaaattg taaaattaat caaaatgctt atgatacaag acagttcaaa
    24481 acaattgtgt agcacagata aggagttttt aaataaagct caaaaatttg ctttatcaga
    24541 attagctgct gctcaatgta aatcctatac aatggtatta gaggaaatga aaaaacatat
    24601 attaaattca aagaatacaa attaaaccag ttttttaaac tggttttatt ttttatacta
    24661 atcatccatg ctactttagc ttattttaaa ctgcctaatc taatattaat aaagttatct
    24721 caattagata gatgtatttt attcaaataa aaaaaactaa gagtaaatca tattactgat
    24781 ttactcttag ctaataaaat ttaatcttct tcaagttctt ttttctcttg taataataca
    24841 cttaattcat ttgcttctaa aacttgtgcc tctgcaatat caagtaactc ttgttgccat
    24901 tccacacatt tatctttgta atcaaaaggt agcgttctgt caactatgta cttatcaata
    24961 cagtcctccg cacttttaat tatttctttt ctttcatcaa taaatttttc taattctact
    25021 aaggcttctt cataagcttt tctactcata taaattcccc ctttgtacaa ttattctaca
    25081 ttaaaatgat ttttcctctt ttttgtataa aattttattt gtatataact tttccatcaa
    25141 tatcttttat agtttgacat ccagttaata tcatagaact acttagctct gattgaagtt
    25201 tatttacata agtttctaca ccatcagttg caccaccaaa agaagcagtc acaaaaggtc
    25261 ttcctattaa tactgcatct gcacctaaac ctatcatttt tagtacgtct aaaccagttc
    25321 tcactccacc atctgcaagt attgtaactt tacctttaac agcatcagca atttttggta
    25381 aaacttcaca tgctcctggt gtacagtcaa gcactcttcc tccatgattt gatacgacta
    25441 ttgcatccac acctgcttct actgccatta aagcatcttc aacagtcatt atacctttca
    25501 atataaatgg tagtttagta gactttacta actcctttat ttgtttaaca tttttaggaa
    25561 gtactggttt tccatgtaat gataaagtta ctagaccaca ggcatctata tcaactccaa
    25621 ctgcaaatgc accagcttct tcagctaatc ttatcttttt tataatattt tcattatccc
    25681 aaggtttaat aaatacaatt ccagctccat tatacttttt gactacatct aaattttcca
    25741 ttaaaaaagc atctactgcc gtatcaccaa ccattgcata aataccacta ttaacacatc
    25801 ctgcaactac tggttctatg taatctcttt cattaatttt tccacccata tttaaagtag
    25861 ttcctgttac tggagctgca aatataggtg cactcatttt ctttccaaac atctctatag
    25921 atgtatcagg atttgataca tcatgtatta ctctcatatt tacttttact ttttctaaac
    25981 tcttgctatt ttctataaaa gaagaaccac ttcctttacc tcccatgcca ggcacttcac
    26041 ctgcacaagc aaccccatta caaaccttac aaactttaca acttccattt aaattttctc
    26101 tagcactttt taataattca ttatattcca tctttaatcc cccattaact atagtaattt
    26161 atattatata cattatatca tattatattt acagtatatt tttcaaaaaa ctttttctgt
    26221 ggataggagt tatgccatat ttttcaatag cttcatagtg ttctttagtt ccatatcctt
    26281 tgtgagattt aaacccatat tctgggtata ctctatcata ttggtacatt atactatccc
    26341 tagtaacttt agcaagtata cttgctgctg ctattgatat agattttgaa tctcctttca
    26401 caattggatt ttgagatatg tcaatacctg gtattgttgc tgcatcaact aataaataat
    26461 ccggtgcttt ttttaaacaa tttatagcct ttttcattgc catatatgta gcatttaata
    26521 tattaaattc atctatttct tcattgttta ctatacctat tccataatct agtgcttctt
    26581 cttttattac ttcaaaaagt tcatctcttt ttgcctcact cagtttctta gagtcattta
    26641 caccttctat tttggtatct tttttaaata ctacaacaga agctacaaca ggtccagcaa
    26701 gtggtcctct tcccgcctca tcaattcctc caatatataa ataaccttta tcatagcctt
    26761 cgttctcaaa tatatttata gtttccaatc tttcttcttc ttttctaata ttatccagct
    26821 tttttgccag ttttatagct agaccttgta cagattttct ttcatcaact cttaacagct
    26881 ctatatattc catatatttt tctacttcta aagtctctat tatttcttta atctccctca
    26941 cacttttatc ctgcatacca ttccctcatc tttcgttttt aaaaatttaa tttttaaata
    27001 tattaaaatt ttttaattag agtttaattg catccactat agccacattt taaacatata
    27061 ctacatccac ctgtatgtgc aattccttca cttccacact cagggcacac aaatttttca
    27121 tctccttcaa aatttttatt tattatttct ttggcttttt catctttatt atctttttct
    27181 tttttattat ttttataata ttctttttta ctattttctt ttttatcttc tttattattt
    27241 tcttcctttt tttcatttat taaaattcca ctccttttac atccatctct aaatattgtt
    27301 actcctttaa gaccagcctc ccatgcctta atatatatat tgaaaacatc ttcaactgta
    27361 gtttcaaaag gtatatttat tgtagatgat atagaagcat caatatactt ttgccatact
    27421 ttttgcatgt ttattctttc ttcatatcct atattcatag cagtaacaaa aaaatctggt
    27481 aaacattctt catctgtaat acctttcttg tccatatact ctttaacaat tggagtatat
    27541 actttataat acacatcctc atcatgcaag ctttcagttt ttcttatata tgaaagatta
    27601 aatataggtt ctattccacc actgatacct atcattgtag atatggaacc tgtaggaggt
    27661 atggttaaaa gttgtgaatt tctaagccca tactttttaa ccatttcctt agtgtcttca
    27721 tttagatttt ctataaaaaa cttagattta gaaatatttt catatttata ctcactaaat
    27781 gttccatatt ctttagcaat taatgctgat tgcttcacag cttcatttaa catagtctta
    27841 ccaattaaat cacatagttc taaagattca tcagaaccat atcttattcc catttttata
    27901 agcatatcag ctatacccat tacacctaaa cctatctgtc tatacttatg tacactatct
    27961 ctttgttctt gtaatggatg caagttcaat ccctcatcta agacatcatt taaagctact
    28021 acagcttctc taacgcaaga ttttaattta ttaatatcaa aagtagcatg ttcaccaaat
    28081 ggttgtatta taaactctga caagtttatt gagccaagta gacaacttcc tccacttgga
    28141 agtggttctt ctgcacaagg atttactcct gaaaagcaaa agttcttatc ttcactcata
    28201 aaattatatg tttttatttt atcccaaaac aatatacctg gttctgcata atcccagtta
    28261 tttttcacaa atttattaaa taaatcataa gcatttacta tcttaactat tttttctcca
    28321 gtagtttcaa cttcaaaagt acaattatat aacttttttt ctcttacagc ttgcataaat
    28381 tcatctgtta ctcttacaga aatatttgct ttagtaacct tctccaaatc tgtttttata
    28441 tctataaagt cttctatctc tggatgattt acatccatag aaatcataag agctcctctt
    28501 ctccctcttt gaccaattaa gccagttgtc atactgtaaa gttccataaa agagactgct
    28561 ccagtcgtat gttttgcagc attgttaact ttcgctcctt ttggtcttag ctttgaaata
    28621 tcaattccaa ctcctccacc ataactaaaa gttctagcaa gtttttttgc agtatcaaat
    28681 atatcttcta tgctatcttt tggtggttct attacataac aattagaata tgttactttt
    28741 tttccctctt tctgtaatcc tctatttgct aaaattcttc cagcaaatag aaattttttt
    28801 tgtcttatta gttttgatat tcttgaacta ttatttgaaa ttctctttat ccattcatca
    28861 aaactctcac cattatgtct atacttgttc ttccatattg atttttgtaa ttcatcaaac
    28921 tcccaagctt tagtccttat ttcagagcgt ctttctctat ataatatgta gtgttttgct
    28981 atatcttttc tatttgattc cataagtagt acttcaacta tatcttgtat ttcttctacg
    29041 gataagcatt tttccataga attatagtca ttagctattt ttcttgatat accatatgcc
    29101 aactctctat ctactccacc ttcactattt atactagctt tatagacagc atcttcaatc
    29161 ttacttttac taaaaagttc ttttgtacca tctcttttta ttatgtattt catttttaaa
    29221 cctccaataa attagtatta aattaataca cacattaact ataactctaa acaaactttt
    29281 tatatatttg tatattttca tattaattat ctaacataat atatattata ctattttata
    29341 ttcaatataa cactccttat agttttctta acttgtaaaa tatcttccta aaaaactcaa
    29401 aaataatata aaaaagtata taaaag
//
LOCUS       DAYAOX010000020        29354 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.020, whole genome shotgun sequence.
ACCESSION   DAYAOX010000020 DAYAOX010000000
VERSION     DAYAOX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.020"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            55..810
                     /locus_tag="ACGI57_000788"
     CDS             55..810
                     /locus_tag="ACGI57_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9715874.1"
                     /translation="MNNKIDGGETMEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVK
                     LSIQSLADKFFTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKT
                     LELIDYEKINKVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVL
                     HDIGKLGEQDMFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLY
                     CYSTIKQVNGYNISDKVPIMMIMRAIIEQYWLR"
     gene            940..2865
                     /gene="mngA"
                     /locus_tag="ACGI57_000789"
     CDS             940..2865
                     /gene="mngA"
                     /locus_tag="ACGI57_000789"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFG9715875.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIDDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            3009..5624
                     /gene="mngB"
                     /locus_tag="ACGI57_000790"
     CDS             3009..5624
                     /gene="mngB"
                     /locus_tag="ACGI57_000790"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFG9715876.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPDRKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLIAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            5903..7057
                     /locus_tag="ACGI57_000791"
     CDS             5903..7057
                     /locus_tag="ACGI57_000791"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFG9715877.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEKLDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLDSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(7180..9093)
                     /locus_tag="ACGI57_000792"
     CDS             complement(7180..9093)
                     /locus_tag="ACGI57_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9715878.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGAFILSSKKGYKVNLNTNCQTKIDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLVGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            9335..9652
                     /locus_tag="ACGI57_000793"
     CDS             9335..9652
                     /locus_tag="ACGI57_000793"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9715879.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            9675..10907
                     /locus_tag="ACGI57_000794"
     CDS             9675..10907
                     /locus_tag="ACGI57_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9715880.1"
                     /translation="MNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMVFVGSL
                     VTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMETMKNKKFESMSVVAGLAGA
                     GLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGEDSALP
                     DFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFVLLNFL
                     VVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWITLGGL
                     GATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPMWIGTV
                     VISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLLFMIWY
                     PFFKAYEKEEIKLESQEV"
     gene            10936..11289
                     /locus_tag="ACGI57_000795"
     CDS             10936..11289
                     /locus_tag="ACGI57_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9715881.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            11311..12756
                     /locus_tag="ACGI57_000796"
     CDS             11311..12756
                     /locus_tag="ACGI57_000796"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9715882.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            13148..13900
                     /locus_tag="ACGI57_000797"
     CDS             13148..13900
                     /locus_tag="ACGI57_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9715883.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            14002..14922
                     /locus_tag="ACGI57_000798"
     CDS             14002..14922
                     /locus_tag="ACGI57_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFG9715884.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            14925..16841
                     /locus_tag="ACGI57_000799"
     CDS             14925..16841
                     /locus_tag="ACGI57_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFG9715885.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVAKLNNEEIKSV"
     gene            16861..17730
                     /gene="gatY"
                     /locus_tag="ACGI57_000800"
     CDS             16861..17730
                     /gene="gatY"
                     /locus_tag="ACGI57_000800"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFG9715886.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            18240..19703
                     /locus_tag="ACGI57_000801"
     CDS             18240..19703
                     /locus_tag="ACGI57_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFG9715887.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            19707..20879
                     /locus_tag="ACGI57_000802"
     CDS             19707..20879
                     /locus_tag="ACGI57_000802"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFG9715888.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVEVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGEPINNTKKFDMKKIKEISIYLKRGVSPKYVEQSN
                     IKVINQKCIYWRNLKVENCKYYDENLKEKIEDIFLKQNDILINSTGTGTLGRCVKFNK
                     IDNSEYIADSHVTVLRSISNNLNSDYFAALFEFTNIQDTIYRKCVNGSTNQIELSVSK
                     LGEYLIMVPPIEFQNQFSEFVKQVDKLKLKIETNLKELEDNFNSLMQKAFKGELFN"
     gene            20912..21292
                     /locus_tag="ACGI57_000803"
     CDS             20912..21292
                     /locus_tag="ACGI57_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFG9715889.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            21282..21710
                     /locus_tag="ACGI57_000804"
     CDS             21282..21710
                     /locus_tag="ACGI57_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715890.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            21760..25095
                     /locus_tag="ACGI57_000805"
     CDS             21760..25095
                     /locus_tag="ACGI57_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFG9715891.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMMDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTENFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            25476..27539
                     /locus_tag="ACGI57_000806"
     CDS             25476..27539
                     /locus_tag="ACGI57_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFG9715892.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYSNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(27756..28322)
                     /locus_tag="ACGI57_000807"
     CDS             complement(27756..28322)
                     /locus_tag="ACGI57_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3073 family putative ECF transporter S
                     component"
                     /protein_id="HFG9715893.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            28488..29009
                     /locus_tag="ACGI57_000808"
     CDS             28488..29009
                     /locus_tag="ACGI57_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3072 family TudS-related putative desulfidase"
                     /protein_id="HFG9715894.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKIKDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
BASE COUNT        11701 a         3025 c         5319 g         9309 t
ORIGIN      
        1 ttaaattata attatattaa cgaaaatttt agctaatatt tttgaatttt acttatgaat
       61 aataaaatag atggaggaga aactatggaa ttttatgaaa gagcaaaaaa atatgagtat
      121 aaatttacag atacagaaga catgataatt gaatatatga gaaaaaacaa agaagaaata
      181 gtaaaattat caatacaatc tttagcagat aaatttttta ctgtcccaaa taccatagtt
      241 aggctatgta aaaaaatgga gtatgatggt ttctctcaac taaaaaataa tttaaaagaa
      301 gagttaaaaa atgaggataa agagccaaaa aatggttttg aatcagatat aaaaaagaca
      361 ttagagctaa tagattatga aaaaataaat aaagtagcag atataattca aaaatctagg
      421 agtattatag tttatggaat aggtcaaaac attcctttat gtgagatatt agtaaaagaa
      481 attagaggtg ggcatgataa tataacttac tatagtcaaa gacatgaagt acttcatgat
      541 ataggaaaat taggtgaaca agatatgttt tttgcactta gtttaagtgg agaaacgaaa
      601 gaagttatag aagcagcaaa gacagcagtt aataagggtt gtaaggtagt agctttaaca
      661 catatatgtg aaaatcaatt gcaacaaatt gcagatataa gtttatattg ctattcaaca
      721 attaaacaag taaatgggta caatatatca gataaggttc caattatgat gattatgaga
      781 gcaataatag agcaatattg gttaagatag agatttgtgt ataaatacac aaatctttat
      841 tttttaagaa gaaattgtgt gtataatttc aaaaaaacat tttcataatt tattttatgc
      901 tatattcata gcattaaatg atttaaggag ggcgtaattt tggaatttaa gaaaatcaca
      961 aatgaaaacc ttgttttttt aaaacaaaaa ataaaaacaa aggaagacgc aataaagttc
     1021 ttagcaagaa agctatatga tgaaggcaag ttaacatctt atgaggaata tttagaagct
     1081 gtaatgtaca gagaaacttt atctccaaca ggttttgaaa gagggcttgc aattcctcat
     1141 ggaaaatcta agtcagttaa agaagctgca tttgcagcta tgaccttaga gacaccaata
     1201 gaaaattggg aaagcgtaga cccaaataat aaagtagagc taatattttt aatagcagta
     1261 ccagaaagtg aaggtggaaa tacacacata caaatacttt ctgagttagt tactagactg
     1321 tcaaatgaag attatatgaa tatgttgtta aattcaaaaa ctaagaaaga gttatttaat
     1381 aacttagata taaaagaaaa gaaagaacaa ataaaagaag agattaggat tcaaaatgaa
     1441 tcaaaaaaaa ttatattagc aataacagca tgccctgccg gtatagcaca tacatatatg
     1501 tcagcagaag cattaattaa agctggtaaa gaaataggtg tggatgtgta tgtagaaaag
     1561 caaggagcta atggtatggt ggaccctcat acgccagata taataaaaag ggcagatgca
     1621 ataatatatg caacagatgt agcacctaaa aatacagaaa gatttgaaca tttgccaaac
     1681 ataaaaacgt cagtagcagc tccactaaaa agagcaaaag aaataatata cgaagcttta
     1741 gaagtagcac aaaaacaagg taaaggtgat tatatagaaa aatcatctga tatatcatat
     1801 tgtgaaaaat catcatggaa aaaagatgta aaatcagcaa tacttacagg agtatctcat
     1861 ataataccac ttattgtggc aggaggtatg atacttgcat ttgcagtatt agcatctcaa
     1921 gcatttggtc tacaagaact atacaatcaa gaaggttctt ggttatggca attaagaaag
     1981 ctttctggag gtatgttagg tacattgatg gttccagtat tatcagcata tatgtcttac
     2041 tcactagctg aaaaaccagg tcttggacca ggatttgcag caggtctagc agctgattta
     2101 ataggtagtg gatttcttgg aggtatgtta ggaggtttac ttgctggtta tatagtaaaa
     2161 tttttaaaga agaatatacc agccaaaggc acattagctg gattcgtaag tttttgggta
     2221 tatccagtag ttggttcttt ggtagtaggt ttattgatgt tatttgtagt aggaaagcct
     2281 gtagcagcac ttaacaatgg tcttataaat tggttaaatg ggatgactgg agtaaatgca
     2341 atactactag gtgcaataat aggagctatg gtttcatttg acttaggtgg accaataaat
     2401 aaagcagcct atacatttgc aataggagca atagcaagtg gaaattttat accttatgca
     2461 atatttgcat cagttaagat ggtttcagct tttggagtta caatagctac tgttttaggc
     2521 agagatttat tttcagaatc agaaaaggaa ataggaaagc aaacttggat attaggattg
     2581 gctggaataa ctgaaggtgc aataccattt atgatagatg acccaatccg tgttatacca
     2641 tcattggtag ctggttcagc cataactggg tctatagtag cattcttcaa tataggtctt
     2701 caagtgcctg gagcggggat attctcattg gcgcttttag agggaagtat gtcaggattt
     2761 attatagcat caatatggtt ctttgcggca gtacttggag ctataatatc agcaatatta
     2821 ttaataataa ctaggaaaca aaaaaatcaa agaaagatac attaaaagat acgttattag
     2881 atatttaaat caaaggttta attatagtga taaaataaat attaaaaaat tttgtaaact
     2941 cagtagtatt tttattggat ttttaattag acagaaaata ttatcagata agttttggag
     3001 gataagcaat gaaaaaaaat gtacacatag ttcctcatat gcattgggat agagaatggt
     3061 acttctcaac tgaggaatct agaatattac ttgtaaataa tatggaagaa ataatggata
     3121 tgttagaaaa taatcataac tatccatatt atgtgatgga tggtcaaact gctatattag
     3181 aagattattt agcagttaag cctgaatgta aggagagaat aaaaaaatta gttcaagaag
     3241 gaaggctaat aataggtcct tggtatactc aaactgatga aatggtagtt ggtggagaat
     3301 ccatacttag aaatttacta tatggaataa aagattgtga tgaatttgga gaatatatga
     3361 aaataggata tttaccagat tcatttggtc aaagtgctca aatgccacaa atactaaatg
     3421 ggtttgatat aaaatattca atgttttgga gaggatgttc tgaaagaaaa ggaactaata
     3481 aaacagagtt taattggaag agtgatgatg ggtcttctgt tttggttcaa atacttccat
     3541 taggttatgc aataggaaaa tatctaccaa tgaatgaaga tgagctaaga acaagaatgg
     3601 ataaatattt accggttctt gataagggtg caactactga ccatataata cttccaaatg
     3661 gacatgacca aatgccaatt caaaagaata tatttgaagt tatatataaa ttaaaagaat
     3721 gttatccaga cagaaaattt ttcttaagta gatatgaaaa catatttaaa gagctagaaa
     3781 aaaatgagga tatagataca ataaaagggg aatttttaga tgggaaatat atgagggttc
     3841 atagaagtat attctcaaca agaatggata taaaatcagc aaatgctaga atagaatcaa
     3901 agataactaa catattggag ccactagctt ctattgcata ttctctagga tttgaatatc
     3961 atcatggatt aatagagtta atttggaaag aaataatgaa aaatcatgct catgacagca
     4021 ttggatgttg ttgttcagat aaagttcacc aggaaataat gaatagattt ttcttagctg
     4081 aggaaaaagt agaccaatta ataacatttt acaaaagaag aatagttgat gcaatgagtt
     4141 gtgaaatggc tttagataag ttaatagcat ttaacttaat gccttacgat aggaatgaaa
     4201 tagtgagcgc ccaagttata actaaaatga aagcatttga aatatttgat aaagataata
     4261 ataagttgga ttttgaagtg gtacataaag aagtaataga tgcaggatta atagaccgtc
     4321 aaattgttca ttatggaaac tatgattcat ttatggtata tacaataaac tttaaagata
     4381 aaatacctgc aatggggtat aaggcgtata tgataaaaga agttgaacat atgttagaaa
     4441 aagcttatga agtatgtgac atggtagata atgactttta tactattcaa gttaatgaaa
     4501 acgggacatt aaagatattt gataaaaaat taaataaaac ttttgaaaat gtattattga
     4561 tggaaaatgg aggagatgaa ggagatgagt atgatttttc accacttcca aatgagaaat
     4621 taatatttaa cgataatata gtagcaaatt ccactataaa gaaaaataga tttaataatg
     4681 agataaagat aaattataga ttaaaagtac caaaagatat acaagctaga aaaaataata
     4741 gagcagattc atttattgac tttgatatag ttataaatgt tccaaatgat aaagctataa
     4801 tagatgtagt attcaacata aataaccaag caagagattg tagggttaga acttatatac
     4861 caacaaatat agcttcaaag ttttctgtgt ctgataatca gtttggacat ataaagagaa
     4921 gtgtgtatga tgatgctatg gaagtttggc aacaggaaga ctggagtgaa agaccagatt
     4981 ctatatatcc aatgctaaca ttcgttggat tgtctgacaa agagcatgga gtagctgttc
     5041 ttacaaacag tacaagagag tttgaaatag taggggaaaa atttgacaca atagctataa
     5101 cattatttag aagtgtagga ttcctaggta aagaagaaat ggcaagacgc ccaggtcgtc
     5161 catcaggaat aaagttgcct actccagatt ctcaaatgat aggagatata aaaatagact
     5221 tagcaatagt tactcatgaa aaatctacac tagaagcaaa tgttgcaaat atggctaaag
     5281 agtatttaac aaaaatacaa acttataata aaatgccata taatgctatg aaattgaatc
     5341 aatcagaaat agaagtagat tattcatata gtttacttaa ggaaactaat aaggaccttg
     5401 tgttaagtgt tgtaaaaaag gctgaaaaag aagaggcatt agtagttaga ttttataatt
     5461 ctaaagaaga aacaaaatca gctaacatag tatttaacaa aaatattaaa acagctaaat
     5521 ggattaacct aaatgaaaaa gaattagaat ctatatcaat agataataac tctgtaaatg
     5581 tagagtgctt aatgaaccaa gttaaaacta tactggttaa atagtgctaa aaatgattag
     5641 atagtgctaa aaaggaatta tcttaaaata gagataatcc tccttatcat tgttgaaata
     5701 ataaacatat atttgatttt atacaaaaga gtgtctcatg aactaagaaa tttattttga
     5761 gacactccct ttttataaga taaaagaata ttaatggtat aagttgtata caaagagaga
     5821 tgtagagtat aaaataaaag ataaattaag cttataaaat tatatgttta aaataaggag
     5881 taataaaaag gagtagataa gtatgaaaat attaatttct atagattctt tgaaaggaag
     5941 cttatcttca atagaggcag gaaatgcaat aaaaaagggg atattgaagg ttaaagaaga
     6001 tgcgcaagta aagatattgc cacttgctga tggtggtgaa gggacagtag atgctttagt
     6061 tcaagggatg aatggtaaaa aagagactat agaagttact ggaccaatag ctaaaaaagt
     6121 ggatgcaaca tatggattat taaaaaatac aagtacagca ataatagaaa tagctcaagc
     6181 atcaggatta acattggttc cgactgaact tagaaatcct ttatacacta caacttatgg
     6241 tgttggggaa ataataaagg aagcaataaa taaaggctat agaaatttta tagttgggat
     6301 aggtggaagt gctactaatg atgctgggat aggtatgctt caagccttag ggtttgagtt
     6361 ttatgataaa aataacaagt tagtaggatt aggtggaaaa gttttaaatg aaataagaca
     6421 tataaaaata gaaaatagat taaaggaatt agatgaatgt aagtttaaaa tagcctgtga
     6481 tgtaaataat ccgctgtttg ggaaaaatgg agctgcatac atatacgggt ctcaaaaagg
     6541 tgcaacatca gaaataattg agaagttaga taatgggctt agaaattttt ccaaagtagt
     6601 taaaaattac ttaagtaaag atgtagctaa tgtagaagga gcaggagcag caggaggatt
     6661 aggttttgct tttttagcat ttttagattc aaaattagaa tctggaataa aaataatatt
     6721 agaagaaatt aaattagagg aagagttaaa agatgcagat tttgtcataa ctggtgaagg
     6781 tagattggac aatcaaactg ctatgggcaa ggctccaata ggagttgcaa aactggcaaa
     6841 aaaatatggg gtaaaagtaa taggtttggc tggagccaca acagaagatg cagttaaatg
     6901 taatgaagag ggtatagatg cttatttttc tatagtaaat agagctatga caatagaaga
     6961 agcaatggat aaagcaactg ctagtgagaa tatgacagct acaacaacac aaatattcaa
     7021 tcttataact agtatacaac agtcaaaaaa tatgtagtat taaaacaagg tatattaggt
     7081 aaaaagctgt cacatattaa tttagacttt ttagtgtgac agcttatcta ttttatgtta
     7141 ataacttaag aaacatatat tggaaataaa ttaatggatt tagatatttt gaataagata
     7201 agctattcta ttcataaaac tactatagtt atctgcctta gtaagttctt taacattatt
     7261 ccattcagat aatatttcaa taaagctttc aaatatgtaa ttgaattttt ctttttcttt
     7321 ttcatttata ggtatcatag ctataatttg aactttttcc tcaccccatt taactggttt
     7381 atcgttaaca attaaacata ctccggtttt taaagcatcc attttcatgc tatgaggaat
     7441 tgctacattg ttattaaatg cagttgaaga cattttttct cttataagaa catcatctat
     7501 ataattgggt ttgacatatt tattttcgta cagaatattt cccataaatc ttatataatc
     7561 tttagcacta tctaaatata tatttttcat aaacaaagat tcactaaagt acatttctaa
     7621 atgattttgt acagtaagta tattttttct atctttaatc tgaataaatt tactttgaat
     7681 attatcataa tcctttctat ttaggtaagg atttacataa acaaaaggta tagggcatct
     7741 atcgttaatt ggtaaagtag taataaccat gtctacctta ttgtcaaaat caagattatg
     7801 agtatctgaa aaaatctgaa taatttctat ggattcatta aatctcttct caaggtctct
     7861 tactattttg aattgtaaat catagtaatt tggagtaatt aatgcacata gaactttatc
     7921 ttctaataat ttttgtctat caaagaatga acctacatga agagatatat aagatatttc
     7981 atcttcgtct atatctacat tttctaaagt ttgaatctga ttgcaaatat aaacagatat
     8041 atcatatatc aaagggtagg aatcttttaa cttttgaggt atttgatttc ttaaaacttg
     8101 attatttttg gctctactga ttaaattttt tacatgaaga gtaaatttta caacaaattc
     8161 ctcatcagat aaatctataa gatataagtc atatacattt ttaattattt ttttagttaa
     8221 tttaacaaag tgttcatcta tataatttga caatgattta gtatccattt gattgtagtt
     8281 taagactgta gttttgtttg ttaactgaaa tactaaatag tataattccg cactagttaa
     8341 agttatattg tattcagatt ctaaaaaatt tgatatgtct tttgcaatat ttatatctac
     8401 gttattgtct atgtagttta ctacttcaac agagtctatg taattattct tttttattct
     8461 gtctatagtt atcattaaat gaagaagtat gttgtttata gcatactcat ttataaataa
     8521 attgtatttt tttaatatat atattatatt ttcttttagt ttagtcaaat tatactcttg
     8581 atatattttt tgtacatcaa ataagcttaa aaaatcactt cccgtttctt taaatattat
     8641 agagctcatt aacttgcgct ttgcagactc atttccaact agtttcaata aaaatttcga
     8701 ttgctttatc tttaaattat aacttcctat aaatttatta gctttgacta tatccccttc
     8761 aatagtagct gggctaacaa ataattcttc acttaaatca aatatattaa ttccattact
     8821 attaactata agttctttta aaattaaatt taatctgtta tcaggtgata tattctctga
     8881 actatcaatc ttagtttgac aattagtatt gagatttact ttatagccct ttttagaaga
     8941 taatataaat gcaccattag aattaatctc gtttacatat tttctaatcg aacgtgttga
     9001 tacattaagg taattagcta atgtggatga atctatccaa tcatgttgcg tttttatgaa
     9061 atttaaaaat agctcttttt tagatgtttt cattagattc cctcgcatag ttttaaaata
     9121 aaatctgtat gatatgataa ttatataatt gaatgtacgt attataagat acattatact
     9181 atattaatag gtctaattaa gagtaaatga tttcctatta ttaggaaata tatttatgtg
     9241 aaatgaactt atttgggaag taaaatataa acataataaa ataaaaagtt cattaagaaa
     9301 taaattaatg gataaaaata ttggaggtat tattatgaaa aatataagag tgcttttggc
     9361 gtgtggttca ggagcaagta caggttttat ggcaactagt atgagaaaga gtgcaaagaa
     9421 aaaaggtata gaagcagata taaaagcgag aagtgagtca gagatagata agtatttatc
     9481 agaaatagat tgtttattac taggaccaca tttagattat atgaaggaag aaattcaaca
     9541 aaaggctaat gaatataacg ttccagtagc atgtatatct aaaactgcat atggaagact
     9601 agatggtgca aaggctttgg aagaagcact agaactaata tcaaacaaat aaaatattta
     9661 tggggagagt gtttatgaat aaatttatag attttttaag tgaaaaattt actcctgttg
     9721 taaataatat gactaagaat atatgggtac aatcagttca atcaactata atgaaagttt
     9781 tgccaatggt atttgtaggc tcattagtaa ctatagtttc tgtcttgaag aattacataa
     9841 gctttttacc tgatttaagt ccaataaatc aatatacatt tggactttta ggattattta
     9901 tagcattttt attgcctatg gaaacaatga agaataagaa atttgaaagt atgagtgtag
     9961 tagctggtct tgcaggtgct ggattaatgt taatgatgat tcgtccagag attacgaatg
    10021 aaggagctat atttaacttc aacaggtttg gaggagaagg aatgcttgta tcgctagttg
    10081 caggattatt ctctggatta ataatgtctt tatttggttc tttttcattt ttcggagaag
    10141 attcagcact tccggatttt gtaagcaagt ggtttgataa tatgttacct ataacagtga
    10201 taatgtttgt aggctgggga ttaatttatg gactcaactt tgacatgttt gaaataatag
    10261 ttagagtatt taaaccagta tcagatatag cacagacatt acctggattt gtactcttaa
    10321 acttcttagt agtatttata tattctttag gaataagtgg gtgggtacta tctcctataa
    10381 cttattcaat tcaacttggt gcaattgcag ctaatgcagc agcagtagca tcaggaggag
    10441 tagcaactaa tatatatgtt tacgaagtta ttcaaacagg ctggataact ttaggagggt
    10501 tgggtgctac attaccatta gttataatga tgtgtttatc cagagttgga agaataaaag
    10561 caataggaag agcatgtatt gtcccttcta taatgaatat aaatgaacca gtaatatttg
    10621 gagctccagt ggcatggaac ccatttttaa tgttacctat gtggatagga acagtagtaa
    10681 tatcaataac tacttattta tcaatgagta tgggatttgt tacaattcca agtaaggtgt
    10741 ttaatttatg gtatgttcct tttggtatat ctacttttat ggtaaatcaa gatgttagag
    10801 gtcttattgt tttagcaata aatatagtat tgctatttat gatatggtat ccatttttta
    10861 aagcttatga gaaagaagag ataaaattag agagtcaaga ggtttaaatt tagaaattga
    10921 aaggaaatta taaatatgac aattgaagaa catataatag atgaagaatt agtagaagta
    10981 gcaatgcaaa taatacttcg tgcaggtgag gctagaacag aaattaaaca tgctcttaat
    11041 gatttggaaa agtttgatta tagaaatgct gatttaaaac tagctaaagc aaaagggttt
    11101 atgacagaag cacatagagc tcaaaccaag attatacaag gggaagctgg tggagagaaa
    11161 agagcacatt cgctgttatt tgcacatgct caagatactc ttatgaccat attcagcgag
    11221 ttaaatataa ctatgtcttt agtaggaata gtaaagtcta tagaatataa ggtaaataat
    11281 agtaaatgat ttaaataaat aaaataagga gtgattaaat tgggtaagtt tcctaaaggt
    11341 tttttatggg gaggagctgt agcagctaat caattagaag gtgcatggaa tgaaggtggc
    11401 aaaggttggt gtttagctga tgtacatatg tatgataaag atagagatat atctaaacct
    11461 tatgagtctg atatgactat agaaagaata aagtttgcta tgaaagataa taatggatat
    11521 tatcctaagc gtagtggaat agacttttat catacataca aagaagattt aaagcttttg
    11581 aaagagctag ggttaaagtg ttttagattt tcaataaatt gggctagaat atttccaaat
    11641 ggagatgatt tagagcctaa tgaagaggga ttaaagtttt atgataatct tataaatgaa
    11701 gtaatagata atggtatgga acctattgta acactacttc actatgagac tcctttgaat
    11761 ttagtattaa aatataaagg atggtctaat agaaaagtgg tagatttctt tgttagatat
    11821 gcagaattaa tactaaatag atataaaaat aaagttaaat attggatagt tataaatcaa
    11881 ataaatttaa tacattatga atcatttaat tcagttgcat tttgtgtaga ccaagttgat
    11941 aatgtagaag agactagata tcaagctatt catcatcaat tcatagctac agctttgact
    12001 aagaagcttg gaaaagaaat aaattcagat atgatgatag gtatgatgat ggcagattgt
    12061 acatcttatc cagaagattg tgaccctgaa aatgtagtat ttgctttaaa aagaaatagg
    12121 ctacaatatt ttttcactga tgtaagtttt aggggagagt atccagtcta tattaagaga
    12181 tttttcaaag aaaataacat aaatatagtt atggaggaag gtgatgataa aatcttaaaa
    12241 gaaaatacta tggattttct agctttatca tactattatt ctacaacagt atcagcaagt
    12301 aaaaacacca tgaatccaac tgacaatata aagaatccat atttaaaagc aaatccttgg
    12361 gggtgggcaa ttgacccaaa aggattatac aatactttat ctcaatatta tgatagatac
    12421 caaaagccaa taatgatagc tgaaaatgga tttggaatgc atgataaatt tgaagatgga
    12481 aaagttcatg atagttacag aatagattat ttaggaaaac atatagaaca aataggtgaa
    12541 gctatagctg atggaaccga aatattagct tattgtatgt ggtcgccaat agatatagta
    12601 agttgttcat ctcaagaaat ggaaaaaaga tatggattta tatatgtgga tatagataac
    12661 aatggaaatg gaagtaaaga acgttataaa aaagatagtt tttattggta tcaaaatgta
    12721 ataagaacta atggagaaga attaagagaa aagtaaatag tttttaaata taaacaaccc
    12781 cccttataaa tactgtatat attattaata tagttttata aggggggtta taattgtaaa
    12841 taaaatgatt tattaattat atgattcata gcgaaattta tatttatact tttaatcata
    12901 aacatatcag tatgaggaat atctattgta cttcatccaa attttggtag aggtacaatt
    12961 cttttttatt ttaaaatgct ataagataac ataataaact ttaagcaaaa aattattata
    13021 ttataaattt acatatatag acattgacta aatcttaatt gtatataaaa ataagtagtt
    13081 aagaggtaat atattatatt actcgataaa tttagacaaa taaaacttat ataaggggag
    13141 ataacatatg ataattgagc taaacaaaag taattctgaa aatctaacga agacggaact
    13201 tgaaataatt aaatttataa atgaaaatga atcaatatta ccagaattat ctatagtaga
    13261 gattgctcat gaaacatatt catctccagc aaccgtttca agagcaatta gaaaatgtgg
    13321 tttaaatgga ttcaatgaac ttaggtatag attaacagta aaagatgaaa atgatgatat
    13381 acaaaatatg ggggatataa taaataaatc ctttatagaa gcacagagag ttatagaaca
    13441 aatatcttta actacactaa tcaatataat aaaaactata aataaatcta gtaagatata
    13501 tattttagca agagggctta ctgaatatgt agcaaaagag tttagtctta aattgcaatt
    13561 acttgatttt aatgttgttt ttataaatga ccccaacata atgaaaataa aatctaaatc
    13621 aataaaaaaa gacgaactta taattatatt ttcattgaat ggaaatacca tagagcttgt
    13681 agaatcagca cagaatgcca atttagttgg agctaaggtt ataacttgct gttgtagtga
    13741 aagtgcaaaa cttataaagt attcggattt atatattgta ggatacaaac atgaacatat
    13801 atcaatttct gaatatgaag tatcttccag attgcccttg tatattatat ctaggataat
    13861 tatagattat atggtcagat ataataattc tgaaaattaa attgaaaagt ctatgaaaat
    13921 cgattttcta tttgtgaaaa gattttcata gactttttta ttataatgaa aacatacaca
    13981 gcacaagcaa gggggaagac aatgatatat acattaacta caaatcctgc aatagatatg
    14041 aatatatcta ctaatgggat aaagagaaaa ttagtaaatc gtacatcaaa tgctgtatat
    14101 tctcctaatg gaaagggatt aaatgtaact tttgtactag gtcattatgg tatagaatct
    14161 aagatattag gattttttgg aggattttca ggaaaatata tagttgaaga aagcgaaaag
    14221 agaggatttg acgtattgcc tacttgggta gaagatacaa ctcgtataaa tatattttta
    14281 aatgatggaa gtgatgaatt taaatttgta aactctgggt catatgtaaa tgaaaaacaa
    14341 aaactagata tgctagataa gatacagtca ttagcagata taagttactt atcaataagt
    14401 ggaagtctac ctcctggtat tgatgataat tactatgagg atatatttaa aatttgtaaa
    14461 aataaaagta taaagacaat actcgatata agttcaccaa aattaaagga gttactacag
    14521 tataatccat atttgataaa accaaatgat gaagaaatca aagatatatt tggaattatt
    14581 gtaagagatg aagaagatat taaggatgta cttaaattac tacatttaaa aggtgctcaa
    14641 aatatattgc taactttagg agaaaaaggt tcttattttt ataatggaaa atcaatttat
    14701 tatgctagtg ctcaaccagt tacagtagtc agctctgcat gtgctggtga ttctgcatta
    14761 gctgcttttt taagtatatg gttggaaaat ccagaacata ttgaagaagc attaaaaagg
    14821 tcagcagcaa caggagcaag tgtggctgaa agtaatggta taggtaattt ggagaatgtg
    14881 gaagattata taaaaaatat aagagtcaga aaggtggtat aaaaatggga aagaaaatga
    14941 ttctaggggt aacaggttgt ccaactggaa tagctcatac ttttatggca gaagaagcat
    15001 taaaaaattc agccagggaa ttagggtgtg acataaaagt tgaaacgaat ggagctatag
    15061 gtgtagaaaa taagttaaca gcaaaagata tcgaaatggc agatgcaata atagttgctt
    15121 gtgataaaaa tgttgatatg gacagattca atggaaagcc agtaatagaa gtacctgtca
    15181 aagaaggtat acataaagct aatgaactta tacaaagatg tattgatgga aaagtagcta
    15241 ttagaaaagg aaacttatca agcagtaaaa ttgcagaaga aggaaattta tcgtttggtc
    15301 aaaagttgta taaagactta atgaatggtg tatctcacat gttaccttta gtagtagcag
    15361 gtggagtact tactgctata tcatttttat gggggattta ttcatttgac ccaaattcag
    15421 aacaatataa tcaaatagca gctacattaa aatctgttgg tggatattct atgaacttga
    15481 tggttcctgt attggcagca tttatagctc aatctatatc gggaagagct ggtatgttag
    15541 caggacttgt tggaggaatg atatcatttg atacaggttc ggggttttta ggtggaatca
    15601 tatcaggatt tttagcaggt tacttagtta aactatttgt acatttgtta agaaaattac
    15661 cacgacaatt agaaggtctt aaatcgattt ttataattcc tattgtcagc gtaggtcttg
    15721 tgggtgtagc aatgttgtta cttggtgggc catgttcagc acttaacaat gctatgatga
    15781 actttctatc ggggttacaa aattcaagcc ctataatttt gggaatagta ataggatgta
    15841 tgtcagcatt tgatatggga ggtccagtaa ataaagcagc atatgtaact ggaactatgc
    15901 ttctaggaca gggaaattat ttatttatgg caggtgtatc tgcagcttgt ataacaccac
    15961 cattggtaat agcaatagca tctactttat ttaaaaatag atttacagaa gaagatagag
    16021 cagcaggatt aatcaattat atactaggta gtactcatat aacagagggg gcaatacctt
    16081 ttgcagcaaa aaatccatta aaagtgttgc cagtacttat gattgcatca tcaatatcat
    16141 caattttaac ttatataatg aaaatagaag taccagctcc acatggaggg tttttggttc
    16201 taggattagt caataagcca cttttatggg tagggtgtat atttgtaggt tcattagtag
    16261 gtgctgtact ttatatgatg gttacaccta aagtagtaaa tgagaaaaat actatagaaa
    16321 atacaaatgt aacaaaagat gaacataaaa atgaaagtgc agaatcggaa aacgtatcta
    16381 ataatatctc tttatatagc gaagaaacag taattttaga tgttaaggga aaaaataaaa
    16441 cagatgtaat tgatgaaatg attggaatac ttgataaatc tagtgttctt ttagataaaa
    16501 agaagtttaa agaagaaatc cttaaaagag aagaaatatc atctacaggt tttggtatgg
    16561 ggatagcgat accacatgca aaaacagatg cagtaaaggt accacgtgta gcagtaggtg
    16621 tatcaaaaga agggtttgac tttgaatcag aagatggaag cttagcacat attatattta
    16681 tgatagcagc tacagataat ggtgataatt tacacttaaa aacattatct caactatctt
    16741 caaaattaat gaatgaagaa tttttaaatg agttattaaa tagcaaaaca agtagagaaa
    16801 tagtagcaaa attaaataac gaagaaatca aatcagtata aacatatagg aggagaaatt
    16861 atggcattag tgacaactaa acaaatatta ttagatgcac aagaaggaca ttatgcagta
    16921 ggtgcattta atgttgaaaa tatggagatg gttatggcag taatagaggc tgctgaagaa
    16981 cttaaatcac cagtaatatt gcaaacaaca ccttcaacaa taaagtatgc agggctagat
    17041 tattatttag cgaatgtaaa ggtagcatca gagaaggcaa gtgttccagt agcaatgcac
    17101 ttagaccatg gttcaagctt tggactagct atgcaggcac ttagagttgg atatacttct
    17161 attatgattg atggttctca tgaaagttac gaagataata tttcaatatc taaatctgtt
    17221 gtagatgcat gtatgccttc agatatacct gttgaagctg aattaggtaa agttggaggt
    17281 aaggaagacg atttagatgg tggtgatggg ggagcatata cagacccatt agaagcaaaa
    17341 gagtttgtag agagaacagg ggtatcatct ttggcagtag caataggtac ggcacatgga
    17401 ttatataagg gagagccaaa gcttgattta gatagattat ctgaaattag agatgttgta
    17461 tcagttccat tagtattaca tggtgggtct ggaataccag atgaagttat taaggaatct
    17521 ataaaaagag ggatttgcaa ggttaattat gcaactgaat taaggattgc ttatagcaat
    17581 ggggttaagg atgtgcttaa ttctgaccca gaggtaattg accctaagaa atatggtaaa
    17641 aaaggtttag agtcagttaa agattttgtt aagagtagga tggaagtttg tggatgtgta
    17701 ggaaaagcaa tagagatagt gaagtgttaa taataaaggg agagaataag taagattaaa
    17761 ttaaggggtg tctcaaaatg aactttttag ttcaggaggc actctttttt gtatgaaatt
    17821 aaatatattt tcatcattcc gaaaataaaa aagaagatta tatctatttt gagacagcat
    17881 cttattttat tttttgattg agattgtaat tgagaaattt gaaataagga agatgatata
    17941 attaaataaa aaatttcttt ctaagtttaa attaatatta aaaagttata atatggtaag
    18001 agttatccag gtagatgact tctatgctgg atatatgata aaataatttt gtatgggatg
    18061 tataataatc ataaaataag agtattgcat tagtttttta actaaaacat gtaaatagga
    18121 aaaataattt aataactgtg tagtaaaatt ttgaatttaa tataagacac taattattaa
    18181 ggcgtacgac taaaatatat atttaaatta ttttatgaac actggaggga aagtcatata
    18241 tgattacagg agaattaagg agcaaagtag acaagatttg ggagacattt tggactggtg
    18301 gattaactaa tccattagaa gttatagaac aatttacata tcttttattt attaaaggat
    18361 tggatgaagt agaaacaaaa aatgaagcag aagcaactct tttgggagtt tcttttgaaa
    18421 ggatatttga tgatgaacat caacatttaa gatggagtaa gttttctaat gaaggaaatt
    18481 cagaaaaaat gtatgaaata gtacaaaatg aagtgtttcc tttcataaaa aaattacatg
    18541 gaaataaaga ctcagcttat gcaaagtaca tgggagatgc aatatttaaa attcctacgc
    18601 ctcttatgtt atctaaaata gttgatggta ttagtaattt agaattaagt aaggatagag
    18661 atacaaaggg agatttgtat gagtatttat tatccaaagt ggctacagca ggtactaatg
    18721 gtcaatttag gactccaaga cacataatag atatgatagt aagacttatt aaaccaacac
    18781 cagaagatat tatagtagac ccggcagcag gttcagcagg ttttttagta tcatcacaac
    18841 aatacttaag ggataatcat tcaagtcttt tcttagtgca agggctgaag gagcatttta
    18901 ataatggtat gttctatgga tttgatatgg atagaactat gcttagaata ggtgctatga
    18961 atatgatgtt gcatggcgtt gacaatccta atatagagta taaggattct ttatcggagg
    19021 ttaatacaga taaagataag tttacattgg tgttagcaaa tcctcctttt aagggaagtt
    19081 tagattatga agcagtgtca gcagatttat taaaagtgac taagacaaag aaaacagagt
    19141 tattattctt ggcacttttt ttaagagttt taaaaattgg tggaagatgc gcatgtatag
    19201 tgccagatgg agtattgttt ggttcaacag gtggacataa atcgataaga aaagaaatag
    19261 ttgataatca taaattggaa gctataatat caatgccaag tggagtgttt aagccttatg
    19321 caggagtatc tacagctata atgatattta ctaaaacagg gacaggtgga actgataagg
    19381 tatggttcta tgatatgaaa gctgatggtt attctttgga tgataagaga aatgagataa
    19441 aggatagtga tatagaggat attattaaga gatttgataa tcttgatggg gaagtagata
    19501 gaaaaagaac agaacaaagc ttttttgttg atgtggaaga gataagagag aatgggtatg
    19561 atttatctat taataagtat aaagaggtag tgtatgagga agtagtttat gatgctccta
    19621 gtgtgattat aggtagagtt agggagttag agaaggaaat agctgttgga ttagaagaat
    19681 tagaaagaat gttagaggta tagggtatgg agtatataaa attaaatgaa ttgtgctata
    19741 taaatattgg caaaacacca tctagaaata cttctgatta ttggggaagt ggtaatagat
    19801 ggttatcaat ttctgatttg aaagaaaaat atatatcaaa atcaaaagaa gagataacag
    19861 atttagctgt tgaaaaagct aatatgaaat tagtacctaa aaataccgtt gtaatgagtt
    19921 ttaagttgtc tataggtaga gttgccatat taaaagaaga catgtttaca aatgaagcaa
    19981 ttgctaactt tcaaataaaa aataatgaac ttataacatc tgaatattta tattatgctt
    20041 tgagaacttt aaattttaat aatactgata gagcagttat gggtgcaact ttaaataaaa
    20101 gcaagttaaa tgatataaaa ataccatatt tcacaatttg tatacaaaat aaaatggtag
    20161 aagttttaaa taaagctcaa gagttaataa ataaaagaaa agagcaaata gaagcattgg
    20221 atgaattagt taaatcaaga tttattgaga tgtttgggga gccaattaat aatacaaaaa
    20281 aatttgatat gaagaagatt aaagagattt ctatatattt aaaaagaggt gtatctccta
    20341 aatatgtaga gcaatcaaat ataaaagtta taaatcaaaa atgtatatac tggagaaatt
    20401 tgaaggtaga aaattgtaaa tattatgatg aaaacttaaa agagaagata gaagatatat
    20461 ttttgaaaca aaatgatata ttaataaatt ctactggaac tgggacatta ggaagatgtg
    20521 taaaatttaa caaaattgat aattctgaat atatagcaga tagtcatgtt acagttttaa
    20581 gaagtatttc aaataactta aattcagatt attttgcagc gttatttgaa tttacaaata
    20641 tacaagacac aatatataga aaatgtgtta atggttcaac gaatcaaatt gagttatcag
    20701 ttagtaagtt aggtgaatat ttaataatgg ttcctccaat tgaatttcaa aatcagtttt
    20761 ctgagtttgt taagcaagtg gataaattaa aattaaaaat agaaaccaac ttaaaagagt
    20821 tagaagataa ctttaattca ttaatgcaaa aagctttcaa aggagaatta tttaactaaa
    20881 caatattacc taataaacaa gaggtgatta aatgagaaat ataccaaatg agattaaaaa
    20941 ctcaataata aatgtatttc caaaagaaaa tactctagca atcataatat ttggtagtta
    21001 tggaacagat aaacatacat cagaaagtga tatagacata gcttggattc ccatcaaaaa
    21061 agtaccaatt acagaacttg caactaagac agaagctcta agaaatatat tagacattga
    21121 tgtagaccta aaaattgtta cagataatta tacaatagca cttaaaaaag ctattttaga
    21181 aggtgaagtt atttatgaat ctaaagattt taaggattat ataaataaat tctattttga
    21241 aaacagtgat gtaatagata tcttagattg gagggacatg tatggtaatt agaaacaaat
    21301 tatcttggaa ctcaatagaa gaagtaaata gggattttgg ttcttgcttg tatgacctgc
    21361 aaaacttcaa aatcttatat gataaggaag aaatgcctga gttatgggct agatacataa
    21421 aagaaggctt taaaagctat atggtatcat ttctagaatt aacaaaagca atgttgtatt
    21481 ataaatcaat tgatttaaac ataaaatcta aaaactttta tgactacctt ttagcttgtg
    21541 agtatcacaa tttattacct aaaaattctt ctatagttat agaaacactt agaaaattga
    21601 gaaatgatga ttctcatgga tatgatatac cacagtttga agatatgtac gaattgttta
    21661 cagaaaatga agaagtattt gttagtataa gaaacagctg taagaaatag tttttagaga
    21721 gatttatttt aactaaggga caaaaggggg ataaaagcct tgggtaattt tgatttttta
    21781 caaaagaaga aaaatttcaa tagttttaat actgcttgct tagaagcaga aaaaagtata
    21841 ctagttagtc catctacttg tgcaactata acaagaagag ctttagagct tgcagtaaaa
    21901 tggctatatg ctaatgatag tgatttagta ttgccttatc aagataatct cagtacgttg
    21961 attcataata atagttttat agaacttata gactatgata tgttaccttt attaaaatat
    22021 atagttaagt taggtaattt atctgtacat actagtgcga acatagagag aggagaagca
    22081 atactttcac ttaacaattt gcatcaattt gttagttgga tagactattg ttattcagat
    22141 gaatatacag cagaagactt taatgaagat ttattacttc atggagaaga gaaaaggacg
    22201 agaccagacg aacttaaaaa tctttacgag agattaagtt caaaagataa aaagcttgaa
    22261 gaagtaataa aagaaaatga ggaattaaga aagtcactaa ctgaaaagag aaaagttaat
    22321 actgtaaact atgatttcaa gatagacgaa gtaagcgaat ttgagacaag aaaaagatat
    22381 ataaatatag aattaaagct tgcaggttgg gaatttggaa aagatatagg ggaggaaata
    22441 gaagtacaag gtatgcctaa tgaaagtggt gtaggatatg tagattatgt tctttatggt
    22501 gaaaatggta agccattagc agtagttgag gcgaaaagaa cgagcaaaga cccaaaaatt
    22561 ggtcaacaac aagctaaatt atatgcagat tgtttggaaa agagatatag tcaaagacca
    22621 gtaatatttt tgactaatgg ttttgatatg tatatttggg atgactattc agatagaaaa
    22681 gtttatggat tttataaaaa atcagaatta caacttatga tggatagaag aaagagtaag
    22741 aagtctttaa gttctgtaac aataaatgat gaaatatcca atagatatta tcaaaaagaa
    22801 gctattcggg ctgtatgtga ggcccttgaa aataagcaac gcaaaactct tttggtatgt
    22861 gctactggta caggaaaaac aagaattgct atatctatag ttgatgtact ttcaagacat
    22921 aattggatta aaaatatatt atttctagca gatagaaaag cattggtaaa gcaagctaaa
    22981 aaaagtttta ctaagctatt acctaacttg gcgttatgta atttattgga tagcaaagac
    23041 agcccagaag atgctaggat gatattttct acatatccaa caatgatgaa tgcaattgat
    23101 gatacgaagt ctaaggatgg aaagagattg tttacacctg gtcattttga tttaattata
    23161 atagatgaat cgcatagaag tatttataaa aagtataagt caatctttga ttattttgat
    23221 tcttacttaa tgggtcttac tgctacacca aaggatgaga tagataaaaa tacttattca
    23281 gtttttgaca tggaaaatgg agtacctaca tatgcttatg agtataataa ggcagtcgag
    23341 gatggatatt tagtagatta tactagtatt gagtttaaga ctaagattat ggaagatgga
    23401 attaagtatg atgagctttc tgatgaagag aaggaagaat atgaaaatac atttaatgat
    23461 gatgagagta ttggagacga aatagggaat aatgcagtaa atgagtggct atttaattct
    23521 gacacaatag atttagtttt aaataagcta atgacagagg ggttgaaaat agaaggagaa
    23581 gaaaagattg gtaagactat aatttttgca aagaatacaa agcatgctag ggcaatagtt
    23641 gaaagattta ataagctgta tccaaagtat ggtgggaact ttgtaaaagc agttgactat
    23701 agtataaatt atgtagattc tataatagat gatttttctg ataagaataa attacctcaa
    23761 atagcagttt cagtagacat gcttgataca ggaattgaca tacctgaaat actaaatctt
    23821 gtttttttca agaaagtgag aagtaagact aagttttggc aaatgatagg tagaggaact
    23881 agattatgtc cagatttatt aggtgttgat atggataagg agagattctt aatatttgat
    23941 ttttgtaata attttgaatt ttttaaattt aatcctaaag gatttgaggg aaataaagca
    24001 gaaacactta ctgaaaaatt atttaatata aaggtaagta tggttaaaga acttcaagat
    24061 attaaatata tagaaggtga gtatgcagaa cttagaaaag aattgttaga agaacttata
    24121 acttctgtaa aagctttaaa tgaggatagt tatatagtta ggatgaattt aagttatgta
    24181 cataagtata aaaatgaaaa tgtttggagc aatattggag cagtagcaca aaatgagata
    24241 agagaatata tatcaccact cataacttca tatagtgatg atgaattggc taaaagattt
    24301 gatgtagtta tgtataatat acaacttgca tacttacaaa ataataatgc aagtaaaggt
    24361 ataaggcatg taatggctac agcagaaaaa ctttcaaaac ttgggactat accacagatt
    24421 cagcaacaaa aatatactat tgaaaaagca atgacagaga atttttggga agattcagat
    24481 atatttgagg tagaagaagt acgtatatca ttaagagagc ttattaagta tcttgaaaag
    24541 tctagtcaaa agatatatta tacaagtttt gaagatatga ttgtagcaga agatagaaat
    24601 gattctgtat ataatgcaaa caatcttaag aattataaaa agaaggtaga gtattattta
    24661 aatagccata aagatgaatt agcaatattt aaattaagaa ataataaaaa gataacaaag
    24721 caagatgttg aaacgttgga ggaaatactc ttaaaacagt taggtaattg tgatgattat
    24781 aagaaggagt ttggagatac tccagtaagt caattagtga gaagactagt gggattggat
    24841 agagaagctg caaatgaagc tttttcagag tttttaaata ataagagctt taatactaag
    24901 caaatacatt ttgttaaact tatagtcgat tatgtagtca agaatggatt tatagaggat
    24961 aacaaagtgt tgatggaaga cccatttaga actgttggaa gtataattga tttatttgaa
    25021 aatcatattg aagagagaaa taaattgatt aaaactataa ataaaattaa agaaaatgca
    25081 tcagagatag gctaatgatg ttatttagtg caaaattatt gttaatgatg tagagaataa
    25141 atttattagt aggtagtcta atattgggtt gaaatagata actagatatg gataatgtaa
    25201 atatacaaag agaggtattg aataattatg caatatctct ttttaattga taaataacta
    25261 tgaattactc atgaagaaga ttaaataata atttttaaaa ttaagtattt gtatatttta
    25321 tagaataatc atgtatttac tttaataaaa taaaaacagg tataaaaagg tatataataa
    25381 ggctttcata tacatttatg agtcaaaatt ttgatataat atatagatac atggagttat
    25441 gaatggaatt tatttaactt atagggggag ttaatatgaa agcaggagaa attgaatttc
    25501 ttagttattt agaggggtca aacaaaagtt ttgtagtacc tgtatatcaa agaaattata
    25561 actggaagaa agaacaatgt aaaagattgt ttgatgattt agaggatata atagattctg
    25621 gatttagaac tcatttctta gggtcagttg tatctatata tagtatggga aaagaatatt
    25681 taataataga tggacaacaa agagtgacta ctgtgtcaat attattgatt gccatgtgtc
    25741 aaataataaa agagtttggg gaatcaaaag gaattattac aacagaagaa cagattaagg
    25801 agtgttatct tataaacaag cactcaagag ataaagatgg aaaaataaag ttaaaaccaa
    25861 tagcagaaga tagaaaagcg tactatgaac tatttgaaga agaaatgaat acagatagaa
    25921 attctaatat agtttcaaat tatttatatt tttataatag aataaaagaa gataatatat
    25981 caatagatga tttgtttgat gctatacaga aattaactat agtagaaata gagttaaaaa
    26041 atggtgaaga tgaccctcaa ttaatctttg aaagtctaaa ttcaactggc ttagatttaa
    26101 gtgaagctga tagagtaaga aactttgtgt taatgggtag aagctctaag ttacaagagg
    26161 aattttataa taattattgg tataaaattg aaaaaaatac aagtagcaac gtaagtagtt
    26221 ttataagaga ctatttgact ataaaggaga gaagtatacc gaataaaaat aaagtatatt
    26281 ttagttttaa aaggtatgta tcagataatg gtatagatat agaaatttta ttaaaagatt
    26341 tacttaaatt ttctaactat tatagagaga tatcttattc atgtactgat gataatgatg
    26401 taaacaaagt tttgaaaagg ataaacaaac ttgaaatatt tgtatcatat ccgtttatat
    26461 tagaattatt tgatgattat aatgaaggta taataaataa aaaagtactt gtagaatccc
    26521 ttaatttagt agaatgtttt atattaagaa gaatagtatg tgatgtacct ccgaatgcac
    26581 tgaataaagt atttatgaac ttaggaaggg aaattaaaaa atttagtgat tataaagaaa
    26641 actattctaa tatattaaga tatattttta taaataagaa atcatctcaa agatttccta
    26701 ctgacagtga attttcaaat gcttttgaaa ctaaagatat atataatact aagtcaaaga
    26761 ataagttata tatacttgag aggttagaga attatagtaa taaagaaatt gttgatttag
    26821 aaaatttaat caataataat atgctaaata tagagcatat aatgccacag acattaacaa
    26881 ataaatggaa ggaatcacta ggagataact ataaggagat acatgataaa tatttacata
    26941 caattggaaa tttaactttg acaggatata attcaaaact aagtaataaa acatttgaag
    27001 aaaagaaaga aatggaagat ggatttaaag atagtaggct ttatctaaat aaatatattt
    27061 catctataga taaatggaat gaagaagaaa taaaaaatag aactaagata ttactagata
    27121 gagctgtaga aatttggaaa tatccaagtg ttacatatga acctccaagt aacagtgaaa
    27181 atatatttac gttagaggat gatgatgtta attttacaaa tgaaagtata gtatcattta
    27241 gtatattagg agagtcatat aaggctaaaa attggacaga attttatgaa caagttgctc
    27301 ttactttata tgatttggat tctgtaaggt ttaacagtat tgtagacaaa acttataaaa
    27361 gagattttat agataatatt aagtctagag atgcatataa attaagaaaa ggtattaaaa
    27421 tatcagaaaa tatatattta gaaacaaatt taaatacaga ggctaaatta aatataatta
    27481 gatttttact taatgagttt gatatagagt taaatgaagt aatattttcc ataaaataat
    27541 gaaatatatg cattttttgc gtaatagaat attaattata ttaaaaataa aatagcacct
    27601 cttgatatag atttatagct caaatttata tcaagaggtt acgtttaatt aaagtaatac
    27661 aaatactatc taattaaatt attaataaaa atgttatata aaaatgagta tatagcattt
    27721 taaagagtaa taaaccttac ataataaatg atttattaat atgaactaga agtattttta
    27781 ctatattgtt ttataaaacc tttaggcatt ttacttataa aatataccac cataatacaa
    27841 gacaaaggtt tgtctactat attactaaga atccttggaa agaacgcagc agtaaatatc
    27901 ttctgaccag actgaactaa aaaaccaacc aataaatcag caccacttcc acttaaacca
    27961 ccaaacaata aaactgaaat aggagtacca attaaaggac aaacaataga taaaattact
    28021 cctgtaataa tagcttcttt ataaccaaaa ccttttttct tagaaatata tccaacaata
    28081 agaccaagga caatatttac aagggcaaaa ggtatatttg taggacctga tataacacct
    28141 aaaactaagt ttgtacatcc accaataaga gcaccccaaa aaggacctaa tacgacagcc
    28201 ccaagtacag taccaactgt atctaaaaat aataatggta tatttatcat agatataacc
    28261 atacctaaga caatattaat aactatgcac attgaagaaa atgtgacttt tttagtatcc
    28321 aattataata cctcctaaaa aaatatatac atttatcata gtatagatta taaaagatta
    28381 ctaattgtat atttctatat agaatattgg aaatatagaa caaataaata atataattat
    28441 caaaatatgt agtatcatta aaattaagaa taactaagga gattattatg aaaagaaata
    28501 gagagattgt agtagtatgt cattgtatat taaattgtaa ttctaaagtg gagggattgt
    28561 cagaatttaa gggagcacaa aatatagcga taaatttaat aaataaaggg tatggaataa
    28621 ttcaactacc atgcccagaa atgattatgt atggaataaa taggtgggga catgtaaaag
    28681 aacagtttga caatttgttt tttagagaac aatgtaggca aatgttaata cctcatgtaa
    28741 ggcagtttga tgaatattta aaaaatgatt atgatataaa aagtatcatt gcaatagatg
    28801 gcagtccaag ttgtggttat aataagacat gtagttctaa acgttggttt ggtgagttaa
    28861 gtggttgcaa tgaattgatg gataaaataa aggatataca aatggtggat gggaaaggta
    28921 tttttataga agagctagaa aagctactta aagaatataa tttagagata cctatagttg
    28981 gatttgacga gcttagagaa gataagtaga gatataaata tttattgtct cattatgaaa
    29041 caccattata tttggcagaa aaatatgact gatggacagt acaggaacca tttttaagtg
    29101 gagtaattag tacttataaa gatgtatcca ctagctgaaa gaaaatgtag aaaggtaata
    29161 aatgaattaa gaagtagata atatatataa aggagtgtct aaaagtgaac tttttagttt
    29221 atgagacact ctttttttat attaaatcaa gtatattaaa tcaaatatat tttaatcatt
    29281 ttagcaatga aaaataaatt tatctatatt ttgagacaac atctttttta gttttattta
    29341 tttgttgtcg tttt
//
LOCUS       DAYAOX010000021        29019 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.021, whole genome shotgun sequence.
ACCESSION   DAYAOX010000021 DAYAOX010000000
VERSION     DAYAOX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.021"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            46..435
                     /locus_tag="ACGI57_000809"
     CDS             46..435
                     /locus_tag="ACGI57_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9715895.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            428..1519
                     /locus_tag="ACGI57_000810"
     CDS             428..1519
                     /locus_tag="ACGI57_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HFG9715896.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFIPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLLTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            1639..2115
                     /locus_tag="ACGI57_000811"
     CDS             1639..2115
                     /locus_tag="ACGI57_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFG9715897.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            2501..2968
                     /locus_tag="ACGI57_000812"
     CDS             2501..2968
                     /locus_tag="ACGI57_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFG9715898.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(3028..3600)
                     /locus_tag="ACGI57_000813"
     CDS             complement(3028..3600)
                     /locus_tag="ACGI57_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9715899.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(3663..4760)
                     /locus_tag="ACGI57_000814"
     CDS             complement(3663..4760)
                     /locus_tag="ACGI57_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFG9715900.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSTEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            complement(4774..5463)
                     /locus_tag="ACGI57_000815"
     CDS             complement(4774..5463)
                     /locus_tag="ACGI57_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFG9715901.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            complement(5628..6794)
                     /gene="rpoD"
                     /locus_tag="ACGI57_000816"
     CDS             complement(5628..6794)
                     /gene="rpoD"
                     /locus_tag="ACGI57_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFG9715902.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            complement(6812..8602)
                     /gene="dnaG"
                     /locus_tag="ACGI57_000817"
     CDS             complement(6812..8602)
                     /gene="dnaG"
                     /locus_tag="ACGI57_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFG9715903.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            complement(8857..9642)
                     /gene="aroF"
                     /locus_tag="ACGI57_000818"
     CDS             complement(8857..9642)
                     /gene="aroF"
                     /locus_tag="ACGI57_000818"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFG9715904.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            complement(9623..10129)
                     /gene="folK"
                     /locus_tag="ACGI57_000819"
     CDS             complement(9623..10129)
                     /gene="folK"
                     /locus_tag="ACGI57_000819"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFG9715905.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            complement(10122..10487)
                     /gene="folB"
                     /locus_tag="ACGI57_000820"
     CDS             complement(10122..10487)
                     /gene="folB"
                     /locus_tag="ACGI57_000820"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFG9715906.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            complement(10498..11289)
                     /gene="folP"
                     /locus_tag="ACGI57_000821"
     CDS             complement(10498..11289)
                     /gene="folP"
                     /locus_tag="ACGI57_000821"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFG9715907.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            complement(11353..11925)
                     /gene="folE"
                     /locus_tag="ACGI57_000822"
     CDS             complement(11353..11925)
                     /gene="folE"
                     /locus_tag="ACGI57_000822"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFG9715908.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            complement(11951..12652)
                     /locus_tag="ACGI57_000823"
     CDS             complement(11951..12652)
                     /locus_tag="ACGI57_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715909.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            complement(12686..13429)
                     /locus_tag="ACGI57_000824"
     CDS             complement(12686..13429)
                     /locus_tag="ACGI57_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HFG9715910.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDIKYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FVKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            complement(13419..14768)
                     /gene="pabB"
                     /locus_tag="ACGI57_000825"
     CDS             complement(13419..14768)
                     /gene="pabB"
                     /locus_tag="ACGI57_000825"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HFG9715911.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            complement(14770..15354)
                     /locus_tag="ACGI57_000826"
     CDS             complement(14770..15354)
                     /locus_tag="ACGI57_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFG9715912.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            complement(15378..16097)
                     /locus_tag="ACGI57_000827"
     CDS             complement(15378..16097)
                     /locus_tag="ACGI57_000827"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9715913.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            complement(16125..17189)
                     /locus_tag="ACGI57_000828"
     CDS             complement(16125..17189)
                     /locus_tag="ACGI57_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFG9715914.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            complement(17333..18142)
                     /locus_tag="ACGI57_000829"
     CDS             complement(17333..18142)
                     /locus_tag="ACGI57_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFG9715915.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            complement(18384..19250)
                     /locus_tag="ACGI57_000830"
     CDS             complement(18384..19250)
                     /locus_tag="ACGI57_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9715916.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            complement(19770..20459)
                     /locus_tag="ACGI57_000831"
     CDS             complement(19770..20459)
                     /locus_tag="ACGI57_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFG9715917.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(20452..20823)
                     /locus_tag="ACGI57_000832"
     CDS             complement(20452..20823)
                     /locus_tag="ACGI57_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFG9715918.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(21005..22402)
                     /locus_tag="ACGI57_000833"
     CDS             complement(21005..22402)
                     /locus_tag="ACGI57_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFG9715919.1"
                     /translation="MEVSIILVKDIKQDIESMPRKILDISLIEDNKSDLTFFNIEEED
                     GDETLKALKQSYVTTNYKEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKN
                     YITSAINMVNKALEINPKDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKY
                     VNLISSEDFKTFLGRYNHSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDK
                     LGNVKKREGYLDKLKELDKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCV
                     LIGMGIYLIQSKKRIENLNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEP
                     EREITVADEEDLYYQALNLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKL
                     LGNKDEAIKYYKKYINTYTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPD
                     SMYNNSKIKEILSEK"
     gene            complement(22533..23126)
                     /locus_tag="ACGI57_000834"
     CDS             complement(22533..23126)
                     /locus_tag="ACGI57_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9715920.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(23191..24660)
                     /locus_tag="ACGI57_000835"
     CDS             complement(23191..24660)
                     /locus_tag="ACGI57_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFG9715921.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(24775..26166)
                     /locus_tag="ACGI57_000836"
     CDS             complement(24775..26166)
                     /locus_tag="ACGI57_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFG9715922.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(26332..26622)
                     /locus_tag="ACGI57_000837"
     CDS             complement(26332..26622)
                     /locus_tag="ACGI57_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715923.1"
                     /translation="MSGLKKILRILVISMMICFAMTFALVLINSVMGVHIGLDYYFLM
                     GSVLTVCLTIGIVLCNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            26846..28975
                     /locus_tag="ACGI57_000838"
     CDS             26846..28975
                     /locus_tag="ACGI57_000838"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFG9715924.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
BASE COUNT         9191 a         5036 c         3059 g        11733 t
ORIGIN      
        1 tatataacaa tcgttttcat ttataaagca taatggaggt tttttatggt tttaaattta
       61 gattttaata gtgataaatc tatttatgta caaattaaag aagaaataac taaggctata
      121 gcttcaggag aattgaaaat aaatgaatca cttccatctg taagaagtat ggctgaaaat
      181 attggtgtaa atttacatac agtgaacaaa tcctacaatg aattaaaaga agagggattc
      241 ttaaatattg atagaagaaa aggtgctatt gtagctcaac taccaatgaa aatagataat
      301 acaactcgtc aaaaaaataa acttgacttg gaattattgg tggcaaaatc ttatttatca
      361 ggtattagta aagaagaatt tttgtcacta agttcaagtt tatttgacaa gtatgaggtg
      421 aaatattatg agtaagattg aatgtctatt tacaatattt ccagttctaa ttttgatgta
      481 tgctacattt tattttatac cctattttgt aggtaaaaaa cacatctatg gagtttctat
      541 agaccaagag cataaaaatt ataattactt tataaagtta gataaagaat ttaaaaaact
      601 attatcttta ggttttatta tagactttat attagttttt attctggtct ttacatttaa
      661 taaattggaa ctttcctatt ttattggtat tataggtttt ttattatatg aaagtatact
      721 atatattcat acacataaaa aagctaagaa gcttaaatca gaaatttatg ctaaattagg
      781 tcatatagat atagattcaa aattaatagt agatatggat tttataaata agaaaaataa
      841 aataataaaa aaatttaaaa taatctattt aataccgata ctatttactt tgggaatgag
      901 tatttttata gcatttaatt ataatcaatt gccagactca attgccactc attggaacat
      961 aaatgggtct cctgatgttt ttatagataa atcatttctt aatgtattta aattaatcgg
     1021 tactgacttt tgtttaatgg ttttattata tataacttcc attggatcca taaagtcaag
     1081 gattaagata gatacaaata gaattgaaga aagtagagtt gaaaacatta aatatttaaa
     1141 taaaattggt tatttgtttt taatattgat gattataatg acaacacagt tttttactac
     1201 tcttttatca ataaaaacaa aactattaac tgctatgaat attacaacgc ttttagttat
     1261 aatttattta atagcaacat atattaattc cccaaattta aaattcaact catcttattc
     1321 tccagaagaa gatgagaagt attggatagc aggcatcatg tataataatc caaatgaccc
     1381 atcattaatg gttaataaaa ggtttggtat tggatggact ataaattttg gcaatcctct
     1441 tggaaaaata ctatatattg ctatagcttt acttttaatt ttttcattgt ttagcctaat
     1501 aaaatctctc ttactttaaa ataccataaa aggtatctta cttgtattat tgggtattat
     1561 attttgtagg ataagaaata tttcatatta tcgttgaagt atagtattta tatattacta
     1621 agaatggaga ttttcataat gttgtcaaaa gctgaaatca aatattttaa tgaatgtgct
     1681 tctgagatac tttcttctga aaaagttcaa cttatgagaa catttcctca tcatggcaat
     1741 gtttcatgtc ttgaacatag tttgtcagta gcatattata gttatctatt atgtaaaaaa
     1801 ttgcacttaa gtgttgatat tcaaagtgtt attagaggag ctttactaca cgactttttt
     1861 ctttatgatt ggcattacaa aggtgataga aaaggattac atggatttac tcaccctaga
     1921 gaagcattaa aaaatgcaac tttatttttt caaataaatg aaaaagaaac ggacataatt
     1981 ttaaaacaca tgtggccact tactgttaag ccacctagat acaaagaagc atttattgta
     2041 tgtttgttgg ataaattttg ctgtttagtt gagactttaa agatacattc tttattatct
     2101 ccttaccatg tataggacaa ttcccacaac acttttataa cttaattttg aagaggtatt
     2161 aagtattata ttcaacttga tacctcttta ctatttatta ttttatataa taatattttc
     2221 tttataatat cgttataaac aacaacttct accctcgata acttcccctt cttatagaca
     2281 tgcttactac cttaaataac aagaaaccag ctattaaatt tattagtatt atttgtacta
     2341 ctctaacaat aatatcataa ataaacctcc aaatagcttc tctttcacta ccttttatct
     2401 taaactatat ttttttctta aaatttgtaa aaaacatagc ataatcttta taaatgttct
     2461 aaaatttaat ttaagcatca aaattagaaa ggatgatatt atgtttaaag aaatgagatt
     2521 gaaaaaaaga gaaatgacaa aagaagatac tgttgaagta ttaaaaaatg gtgaatttgg
     2581 tactttttcc actatatctg aaaatggtta tccttatgga gttgctgtaa attatgtata
     2641 ctttaatgat tctatttact ttcattgtgc aagaaatggg cataagttag ataatatatc
     2701 gaaaaacaac aaagtttctt ttttagtagt tgctaatgaa agtgttattc cagataaatt
     2761 tagtactact tattctagtg ctatagtttt tggaaaagct tgcactgtag aaaatgaaga
     2821 aaagaaaaat gctcttgtag aaataataaa aaaatattct aaaggattct ttgaagaagg
     2881 catgaagtac atagagaagg atatgaactt aacaactgtt gttaaaatag aaattgacca
     2941 tatttctggt aaagcttcac gtttataatt aagtaaaaat acctaaaagt atatacagtc
     3001 gcttttaggt atttttatat gatattctta tatgttaagt ctttttaggt aatctcttat
     3061 acctatcttg gcatcatcat ttggccttat atcagaatta tctcctgcta tatgaagtat
     3121 tccatacgct ggataacctc ctcttactga acatggaact gacaaaagag tcattcccat
     3181 atgttgggca aatatattca tagcttgcaa agctaattct cctcctccat gttgtgaacc
     3241 tgctgtagca aaacaaccaa atattttacc actcaaagga gcacctaccc aaatatcaga
     3301 aaaactatcc ataaacatct tcattggtcc agatacattt ccataatatg ttggtgaacc
     3361 cataaatata gcatcataat ctaatatttc ttttccagat ttaattacat ttatactttc
     3421 aaattcgtct ttgtattctt tggaatttat caaataatat tgaggaagtg tttttgcaac
     3481 ttcgtcactt actctaaata tatctacctc gatattcatt tcttcaagcg cttctttata
     3541 ttctctagct aataaatatg tactcccaca aacactatga aaaattactg ctactttcat
     3601 cattatcccc ctaattaaac tcaaatttat tttttatata gtatttttat tatcaaacac
     3661 tgctaaatag tactaaatgg attcaaatat acattactct ttattacttc aatttcacta
     3721 aattcatcta aaaatctctt cataacattt ttgaatatat cttctgtatc aaaatgacca
     3781 caatccacta tacacattcc aatatcaagt gcatcttgtg cctcatgata ctttacatca
     3841 cccgtaatta gtacttctgc accttgtctc ttagcttttt ttacaaattc tgaaccagct
     3901 cctgttacaa cggctacttt cgttatgtct gtactaaggt ttccaactac tcttatgtgt
     3961 ttcatattta atttctcttt tattttcctt gaaagacttt ctaatgtcat acttttatca
     4021 agtttactta ttctacctag tccaaatatc tcacctttat tttctaactt gtacaaatca
     4081 tatgcaactt cttcataagg atgtgcatct aacatagaac ttattacccc acctaaaagc
     4141 ttctgtggta ctacagtttc aattttaact tcatttacag tttctatatc atttacactt
     4201 ccaagaaacg gattagagcc ttctaatggc ttaaattgtc cttcaccttc tgttgaaaac
     4261 gtacaatcac tataattccc tatatgacct gctcctgaat tacttaaaac ctttctaagt
     4321 tcatctgaat agttatgtgg aacgtatact gctaatttat acaaattttc gcttttagtt
     4381 atatctaaaa cctttgaatt accaaattcc ataatttcca taaaatagtc atttagacca
     4441 tcaaatgcta tatcaaaatt cgtatgcata gaataaagtg atatatcatt tttaataagt
     4501 ttttggataa gtctgccttt taaatctcca ctattaattt tattaatttt cccaaatata
     4561 aaaggatgat gtgtcacaat taaatctata tttttggata tagcttcatc aataacatct
     4621 tcattagctt ctaaagatac caataccttt tttacatcca tatcaaaatc tccaactata
     4681 agacctacat tatcccaatc ctctgctaag tttagaggat actttttttc aatttttcta
     4741 gttagactct ttaatagcat atatacctcc tatttatttt attaaatttt caagtttttt
     4801 tattgcaact tcgctttcct ttctcttctt atctatagct tccccatttt tcccttctaa
     4861 tttattaact atagaactat atgtctttat ctttttttct ataaaatcat taaaaagaga
     4921 atctttgttt tctaaaagct ttattccaac ctcataatat atctcatctt ctattatagt
     4981 atttttacct gtatatttgg caacaattat ctcatatatt ctaaaatctt ctctcactaa
     5041 aacttcttct aaaatttcat atccgttgtt taaaaggtaa tatctaagtt cttcttgtgc
     5101 ttgcattggc tgtaatatta atttttctac attatgtgct acttcttttt tagcttcgag
     5161 aagctcactt atcaagattc cacccatacc agcaattatc acttcttcta cttcccctat
     5221 ttcaagtatc tctataccag aacccaatcg taaatcaact ttatctaaaa gattgttctg
     5281 tatgacttct ttatgtgcat tgtcaagagg tcctttatta acatccgcta gcactgcaaa
     5341 tggtactttt ccctctttta acaaatatac tggtatgtat ccgtggtccg ttcctatatc
     5401 agctattttt ttaccatctg acactaatga tgctattttc aataatctat ctgttaattt
     5461 caaatataat tctcctttca attcccttta tttataggat ttaatccaag ttatagatat
     5521 tagactgaaa taactacaaa aataaaatta aatctttact acaaaatagc catgagatat
     5581 cacttatctc atggcattta tcacaaaaac atcaatgatg tatcttttta gtctaaataa
     5641 tctctaagtt tttttgatct acttggatgt cttagttttc ttagtgcttt tgcttctatc
     5701 tgtcttattc tctctctagt tacatcaaac tcttttccaa cttcctcaag agttctggct
     5761 ctaccatcct caagaccaaa tctaagcttt aatacttttt gttctctatc atttaatgaa
     5821 ccaagtacat cttctatttg ttcttttaat aaagaatatg ctgctgcctc tgctggggct
     5881 ggagcatcat catctggaat aaagtcacct aaatggctat cttcttcttc tcctattggt
     5941 gtttctaaag acacagggtc ttgagctatt ttcattattt ctcttacttt atcttctgtc
     6001 atttccattt cttttgcaat ttcttctggt tttggatctc ttccaagttc ttgaagtagt
     6061 tgtcttgata ctcttattag cttatttata gtctctacca tatgaactgg tattcttata
     6121 gttctagctt ggtctgcaat agcacgtgtt atagcttgtc ttatccacca tgttgcataa
     6181 gtactaaact tatatccttt tgtatagtca aatttttcga ctgcttttat aagacctaaa
     6241 tttccctctt gtattaaatc caagaaaagc atacctcttc ctacatatct ttttgctata
     6301 cttacaacta gtcttaagtt tgcttcaact aacctttgtt ttgctatctc atcaccttcg
     6361 tgcatccttc tagcaaattc tacttcttca tgtggtttaa gtagaggaat ttttcctatt
     6421 tcttttaagt acattcttac agggtcatct atacttatcc cttttggtaa agataaatcc
     6481 acagtttcta tttctattgc agaagagtca tcatgtgaaa ttgcctctgc atcttcatct
     6541 aagtggccta tacttaaatc gtcatcagca accgaaaaat ctatatcagc tttatagttt
     6601 tttgtttctg ttatttctat tcccaaattt cctaaagtct cataaagatt ttctacttga
     6661 tccttatcaa gttcagtctc cgaaaaagct tccattatct ctgcaagtgt cagagaacct
     6721 tgcttttttc ctttttctat taatgtctta gcagtaacct tttttaactc ttttttattc
     6781 gatttatttt ccacactcaa acctcctttc tctacaagct ctttaaaatt ttatttatct
     6841 caactatttt taaagcaatt tccataactc taccatctac ctctttcata tcattattat
     6901 tttctaattc ttgttgttct ctcaacaagc tattaatttg ctcttctaat gagttttttc
     6961 tgatatttgt tattattcct tctatttctt ttgtgttctc taagtttata ctattcaaag
     7021 aaattgaatt taattctttt aagtattctt cggatatgtt taaacttttc aatttgtcaa
     7081 tagttatttt gtccaattct tgatttttaa tcatgtaatt taaaatttct ttactttctt
     7141 tcaataaaaa atcactttct tctactttaa gtaacgctat ttctctaatt tttttgtctt
     7201 ctaacattat ttttattaga gtttcttcaa ctaattgttt tccgtcttgt ctgacttcga
     7261 ctttttcaat tactttttct tctattttct tttgattttt gttattatat ggcttattgt
     7321 aatttttacc ataaacttct ctctttatag actcgacatt aatatctatt tgattgctta
     7381 aatactttgt ataaaaatca atttcaacag gacttgtcaa ttgttttata attttagaag
     7441 cttcttttgc gaacttcact cgttcttcat ctttttgaat gttaaattct tttttaagat
     7501 gatctatttt atattttatg tagtgagtag aaacatccat agctttttta taatcactaa
     7561 gtccgtattt tcttacaaat tcatctgggt ctttagcatc ttttaagtct aacactttta
     7621 cattgatacc taatttagta agtatatcta tagctcttaa agtcgctttt attcctgctt
     7681 catcagaatc ataagatata atagcagtgt ccgcatatct ctttattaaa atgccttgtt
     7741 gttcagtcaa ggctgttcca agggttgcta ctacattttt tataccatac tgataaagtg
     7801 aaattaaatc catatatcct tcaactaaga ctatagtttt actttctaaa ttttttctag
     7861 caaaatttaa tccgtataag ttctgttttt tattaaaaat taatgtgtct ggagaattta
     7921 agtattttgg caaagaatcg tctaatactc ttcccccaaa tcctattata tttcctctat
     7981 aatcgaatat aggaaacata actctatttc taaatttatc atagcaatta gttccatctc
     8041 tattttttgc aattagaccg cactctaata agtcttgttt tttatatcct ttactgatta
     8101 gagtattcat aagtgaattc catgaatcca aagaaaatcc taaaccaaat ttcttaataa
     8161 ttttatcatc tagtcctctt attcttaaat attcataccc aagatttttg cttcccaata
     8221 agtttgaaag ataaaatcta gctgcttccg tatgaatatc ttgaaatttc ttagattttt
     8281 ctattttaat cctagtttct tcattcatgt tagtgttaat ttctataccg catttatttg
     8341 ctaaaatttt tacagcatcc ataaaatcca aattttccat tttcatgaca aaatttatta
     8401 cgtctccacc ttcaccacaa ccaaaacact tataaatctg ttttgatgta tttatataaa
     8461 aagaaggtgt cttttcgcca tgaaatggac ataatccttt gtaattagct ccagattgtt
     8521 ttatattcat atattcagat attatacttg ctatatcgca tctagctttt atttcttcaa
     8581 taatgtcttt tatatcattc atattatcac cttctggtgt aattttttgt gttaacattt
     8641 ctattctaca ctatattcta aatttgtcaa tcactcaata tacacaattc gacttttttt
     8701 attgatatcc ttcttattaa tcaaaaaatt tttatttttt atattaattc tatcaatatt
     8761 aaatctttat ttatattaaa taatctaact atcaaattat aacttataaa tttaccttag
     8821 caacgattac gaataaaaaa gaggctcttg cctcttttaa taattattaa ttgatttcat
     8881 taaatcttca aattcattaa agcaaagaga ttgagcacca tctgacaaag cattttctgg
     8941 gtcaggatgt acttctacca ttattccatc tgctccacat gcaaaagatg ccagtgacat
     9001 aggttttacc aaatatctaa ctccagtggc atggcttggg tctaccacaa caggaagacc
     9061 tgtttccttt tgaataatgg gtaccgcagc taaatctaat gtatttctag tataatcatt
     9121 atacgttctt atacctctct cacacattat aatattacta tttccttcta ttgctatata
     9181 ttcagaagca cctatccatt cagtaattgt ggatgctatt cctcttttta acataacagg
     9241 tttattttgt tttcccactt cacttaaaag agtaaagttt tgcatatttc ttgacccaat
     9301 ttgaattata tcacttatac tatacagttc gtccatatct cttacatcca taagttctgt
     9361 aataacagcc ataccaactt cgtctttaac tgcttttaat atttcaagac cttctttttt
     9421 taatccttga aatgaatttg gcgatgttct tggcttgtat gctccacctc ttaaaatgtt
     9481 tgctccttga ctttttacaa atttagcagt ctccaaaagt tgctcataac tctctattgc
     9541 gcatggtcca gctattataa gtttatcttt ttttacagtt atctttttgt tatttgtatt
     9601 tatctctatc tttgaattat tattaaatag gttttctttc ataacctaca agctccttta
     9661 cttcttctct ttcttcagca gataggaggt ttaaatgttc ttttattgtt ttttcattta
     9721 ttataagttc ttcatttaaa tccataagtg gtatcaaaac gaaagctcta tcttgaatcc
     9781 ttggatgagg tattgttagt ctttcatcag tggaaactac atcattaaaa aatagtacgt
     9841 ctacatctat agttcttggt ccccaatgaa ctattctctc tctgtgtagt tctctctcta
     9901 tttcttgaca atattctaac aactcataag gttcaaactc tgtttctatt tctacacaca
     9961 tatttagaaa atctgcttgt tctgtatatc cccatggttt tgtttcgtat aagcttgatt
    10021 cttttacttt atatatagaa tcactatttt tcaaaagttc acatgctctt gataaattat
    10081 caaatctatc acccatgtta gtacctattc ccaaataagc cttattcatc tcttgttctc
    10141 cttatctcaa caccaaaata gtcataaata ccatttacag gagcctcagg ctttctaact
    10201 ctgaccataa ctccatttat aagtataaat ttattcaaga cttcttcagc aatattttcc
    10261 gctaaagcct ccaaaagatt aaattgttta ttttcagtaa tatctttaac aacattgtaa
    10321 acctcggcat agcttacaga tttatttata tcgtcactaa gacctgcttc tcttgaatca
    10381 atataaagtt ccatgtcgac aaagaatttc tgacctagaa aattttcttc tttaagaaca
    10441 ccatgatatc catagaatcc tagattacta agtaatattt tatccattat attataccta
    10501 ctttctatat attgcatcag tcattctagc tgccattaaa ttttcataaa catcatgtac
    10561 tctaactata tctacgccat ctcttatacc aagaacagta gtagctattg taccttctaa
    10621 tcttttttta ggctcaactg gaagtacttt tcctattact gattttctgg atgtcccaag
    10681 caaaatagga taccctaaat cttttaattc atttaatctt ctcaatactt caatattttg
    10741 ttcaaaatcc ttgccaaatc ctacacctgg gtcaagtact attttttctt tttttacacc
    10801 acaactcatt gccatttcta tacttacctt aaaaaagtct tttattgact ccattatatc
    10861 tttgtcatag tttgtcccat cttgattatg cattatacac acttctgcat catattttcc
    10921 tattacagaa gccatatttt tatcttttct aagaccccaa acatcattta ccatagtgac
    10981 acctaatttt aatgcttctt ctgcaacatc tgccttataa gtgtctattg atattggtat
    11041 atccaattcc tgtttaagtt tttttattac agggattact cttcttagct cttcgtctga
    11101 gtctacatag ctatgacctg ggcgtgtaga ctctcctcct aggtctatta tatctgcacc
    11161 ttggtcaacc atatcttttg catgttgtat tgctatgtcc aagttattaa aatctcctcc
    11221 atcagaaaaa ctatctggag ttacatttaa tattcccatt atataagttc ttttaccata
    11281 atcaaacaca attaatgaca ctcccttaaa ttaaatttta tctcaataaa tgattatatc
    11341 tataataaat tgctacttca tagtaatcaa actcatagcc tcggctctag acgcaatgtc
    11401 cttttcaaat attcctctca ctgctgatgt aacagttttt gaacctggtt tttttacgcc
    11461 tctcattgtc atgcacatat gttcagcttc tactactact aagacaccat aaggttgtaa
    11521 ttcttccatt attatatctg cagcattttt agtgattctt tcttgtaatt gtggtctttt
    11581 tgccaatgta tctattactc tagcaagttt agaaagccct gtaagtcttc ctccttttgg
    11641 taaataagca atatgagctt ttccaaagaa aggaactaga tgatgctcac aacatgaata
    11701 aaatggaata tcttttacca atactaactc ttcatgtttt tcatctgcaa aaagaacttt
    11761 taaatggtct cttggttctt cactaagacc agagaatatc tcttcataca tcctagcaac
    11821 tctatttgga gtttcgatta gaccttctct atctgggtct tccccaattg cctctagtat
    11881 ttctcttact gcatgttgta tcttttcttt atcaacttta ttcatttaaa ctccccccat
    11941 tgatttgtga ctagaagatt agttttataa cctcttttgt ctcaatttct ttaaaaatat
    12001 aattataaac atctaaaagt ctttgatagt cataattttc acttactttt gacactagtt
    12061 caaaaagctc attattttct ttttttaaaa cttttagcaa ttttttgtct tttggtaccc
    12121 atttatcatt tatattaaag tacccaatta ttatatcttt taaatataaa ttcgtcaaaa
    12181 aatgatattc aaaaacatca aatttttctt tattttttaa attcgatatt tttgctccaa
    12241 tatcttgctt tataaaagac ttttcctcta atgacatttt actgggtcct tctaagtaaa
    12301 tatttctact tatatctttt ataatatgtg atatattctc cctatcaaac agtactttag
    12361 catctttcat ttctttcaca aagaaatatt ccctatttga taataatttt tttacaccat
    12421 cttttgaaaa gtaatttaca tcaaattcta ttcccttttt tttaaacagt attctctctt
    12481 gtttttcccc ttcttttaca aaaacgaaca catcaatatc gtttatctct acatcataaa
    12541 gagtaaaatc tacgtcttta ctagaaccca ctaaatatac agccaaagtg ttttggtttt
    12601 ctattatttt agttatctct tcattgtata cttcataaat acaacctatc atcttttgtc
    12661 tccaacaaat tgttaatatt ttaatttatt ctaataactt cactatttta tcaaaaacaa
    12721 cattttcttt attaaatttt attttgtcaa tttctgtaac ctttatagcg cccataagac
    12781 tatttgtgac aaaaacaaaa tcaaaactac taatttctga tatattaatt tcattttcta
    12841 tcaactctat atgtaattcg tcacaaatct ctataatcct tttttttatt attccattta
    12901 atatcggtaa tctatcacta ggagtgtaca ctctttctcc ttttacaaag aaaatattgc
    12961 tcatagaaca ttctaatatt acaccttctg aatttaaaaa tataccatca ttataaccag
    13021 tcttatttgc gatatttttg gtatatatgt tttcaaaata attagtagtc ttatgtctat
    13081 gaattaaact attcccccta actattggag atatagctaa tttaaacccc ttctcatacg
    13141 tctcttgatt atatggaata tccctaatag atatattata accttcatca aaaacagtaa
    13201 ttctaagtgc tttattaatg acattttctt tttttatata tgtaacaatt tcatttatca
    13261 agaaatcttt tttatatttt atatctaaat ccaaacaatt tattgaactt agcattcttt
    13321 ctatatggat atctaaatct actgctattc catttttaac tctaatagtt tcaaacaaac
    13381 caattccaaa tttacttaaa ctactatcaa aacctacatt attcttcata atatcccctc
    13441 aaagccttca ttatggattt agctttatca agtgtttctt gatattcttc atctgggtca
    13501 gaatcccaag tcattcctcc tccaacttga aaatagactt ttttgtcatt tttgattatt
    13561 gttctaattg ctatattaaa atccatatca ccattaaaac ctatataacc tattgaacca
    13621 gtatatacat ttctttgcgt tggctctaat tcgtctataa tttccattgc tctaatctta
    13681 ggtgctcctg ttatagaccc ccctggaaaa gttgctttta ttacatcagt agcatcttta
    13741 tcatctttta actcacctac aactgtggaa actaagtgat ttacatttgc atatggttca
    13801 attacaaata gctctggaac tttaacactt ccagttttag atattctccc aatatcattt
    13861 ctttctaaat ctactatcat aagtagttca gctctatctt tttcactatt tctaagttct
    13921 tgttgaagtc ttaaatcttc ttctttatct ttccctcttg gacgagtacc ttttattggt
    13981 ctagtttcta ttctcttatt aacacatctt ataaatcttt ctggagaatt tgataatata
    14041 tgtgcatctt caaagtttaa gaatgcccca aatggtgctg ggctaaatct tcttaagtcc
    14101 ctatacaatt caaagcttgt aagttctgtt tctccactga acctttgtgt taaattagct
    14161 tgatatatat ctccttgtct tatgtactct ctaacacttt gaactgcatt tttaaattct
    14221 tcctttgtaa aattagattt aagtcttata ggtgttactt ctttttcttc ataacatatg
    14281 ctatctatac cttttttctc ttcttttaat attctttgtt ctatatcatc tattatcttt
    14341 tcttctaatt cgatatctat gtttggagta gctatataag ttttttgcac aagatggtct
    14401 ataactatta catgattata aaatccaaaa tacatatcag gcatctcaat atcatctaca
    14461 gcagttctag gtaaattttc tatgtaatta cctaaatcat aagataaata tcccacagca
    14521 cctcctacaa atggaagatt agtatcattt acaaccctat acttatgtag ttcttctttt
    14581 aaaacttcta aaggattttc atcagtatct ttatacttta ataccttaaa tggttggcta
    14641 cttataaaag aatatcttcc caatttttct ttgtccatag cactgtctaa tataaaactg
    14701 tcatgttcat ttctaaaaat agtaaatatc tcaaaagaat ttaatttagt atttatctct
    14761 cttatcatat tacatatgta ccctttctct agcttctgtt ataaaatttt taagcatttc
    14821 atgaccatac tctgacaaaa tagcttctgg atgaaactgt actccttcta ttaaatattt
    14881 cttatgccta atccccatta tttccccttt atcagtaatt gctgtaattt ctaattcttt
    14941 tggaactgta ttggagtcaa ctattagtga atgataccta gtgacattta aaggattttt
    15001 aagtccccta aaaactcctt tattagtatg gttgatacta tatacttttc catgcacagg
    15061 ctgttgtgct tttataatat ctcctccaaa aacatgtcca atagtttggt gacctaaaca
    15121 aattcctagt ataggttttt tccccttaaa atgctcaaca atatctatgc aaataccagc
    15181 ttcttttgga gaacacggtc ctggagataa cacaattacc tctggattta gttcttcaat
    15241 gtcacttatt ttgatttcat tatttctttt tactacaact gtttccccta attcttctat
    15301 atattggact aggttgtata caaaagaatc ataattgtct atcataagaa tcatactgta
    15361 aatctccccc ctaaaaatta ttttattaac tcagaaactg ttacaaattt aaaacctttt
    15421 tcaatcattt ttggaataaa catatctaaa gcttcaactg tatcattagt tgcataatca
    15481 tgcaaaagta ttatatctcc attttgtact ttattaatta ttttatctgc aatgctatta
    15541 gctcctggat ttttccaatc tttcacatct acacctgtcc ataaaacaac tttatagtca
    15601 tgcttttttg caatttctat taattctttt tctctataac ttccaaatgg tggtctaaat
    15661 aatgtcggtt tttttccagt tacttctttc aaaatgtctt cacacttaac aatctcctct
    15721 tctatctgac tagaacttaa attacttata tcaggatgat tgaaagtatg atttcctatc
    15781 tcgtgacctt ctttacttgc tcttactaat ggttctttat accattttgc atgttttcca
    15841 gctataaaaa aagtagcttt tacattgtat ttctttaata catctaaaac ttgactagtt
    15901 tctttaggat gaggaccatc atcaaaggta attgctatta gctttttttc ccttgaacca
    15961 tgtttaatca aaacatttcc attagtatcc atcatcaaag cagaaacttt actaaaatta
    16021 gatattaaca ctattccaca aaacaagact actgctatta agcacttata tactgtcttt
    16081 tttttccatg atgtcatttt ttaacttcct ttcaaatatt ttagttattt ataaaaattt
    16141 tgagtgtaat atgttttata gcttcctcca aatattactc caactccaat attattatag
    16201 ttccccaaaa tattttttct atgtccttca gagttcatcc aagcttcatg cgcatatatt
    16261 gcattaatct gtcctgctgc tatgttttct ccagctgaag tatatactat accttctctt
    16321 ttcattctgt caaatggagt ttcattcttc ttatttacat ggtcaaaaaa attattatct
    16381 ctcatatctt cactatgttt tcttgaactc aaagttgcct gttcactgta tctcaatgag
    16441 tttaaccctc tttgatttct aacactattt acaaggtcta taacttcaag ttcaaaacat
    16501 tttttaagtt cttcgctatc atctggatat aaatttttaa actcatcttc agtgctttta
    16561 tcaattatta aatatgaaca aactctattt tcttcatgaa tatcgtagaa aattgtaata
    16621 tacttacctt ccttacttat tatatcatat tgattttctg agtttattat atatctggta
    16681 tttccttttc tcttatattt taatggtgta atcttagttc tcactgtttg tctatcttga
    16741 tttagtttta tatcttgatt ttcacatgaa ttaatagaat tactataaag tgctactact
    16801 tcatcatttt caagtccaac cattgcaaat tcttttccat attgattata tacataccaa
    16861 tcaaaaccat attcactaat atcaattcta ccaggtttgc cgatacttga taaaacatag
    16921 cttaaattat ctcctaagct tattttttca aaattcttta tatctgactc tcctaagttc
    16981 tccccttcag tattatttga ttgtaaattt tgactttttt cttttttatc tttcttagaa
    17041 ttatcattta tatttatttt gtctaaaaat tcattccata tttcactttg aaaaaacttt
    17101 tcttttaaat catatccttc ttttaataat tcaggactat aaaataaaac aataagtact
    17161 actaacacta aagcaattaa tctcttcaat atatctctcc ttcttaaaat caatcaaaat
    17221 tttttaatat tgcatttatt aagttaatat tgatagtgta tactaatcaa tatcattcat
    17281 acctatttac agtaagtatt agaaaaaagc tttatctact aattagataa agctacagtc
    17341 tcaaggaaaa tatttcatct aatgttctat caaatataga tggcttatca aatttccaag
    17401 tcccttcata aaccaattca ttatttagat aaaaagttcc ttgcccatgt tttttaccat
    17461 gcataaattc acctatatac tttttacctt tttcccatat ataagttccc ttaccatgaa
    17521 tcaaattatt cttccattct ccttcataaa gtacttcccc tttactatta tagcaaatac
    17581 cgtacccttc catctttcca cctttaaatt cacctgcata tatacatcca tcactccact
    17641 tataaatacc ttttccatct ttttttccgt ttttccaagt tccaatatat tcatctttac
    17701 tactatataa catatttcca ataccgctca tcatatcttc ctcaaaataa cctatatatt
    17761 catttccatt tgaaaatata aagtttcctt ttccattttt tttatcttct ttaaattctc
    17821 ctatgtactc cataactgtt ccttcatcta caaaaaaagt atatgagcct ttcccctcca
    17881 tctttccctc taagaatttc cctacatata catccccatt tgaaaattta tagattttat
    17941 tttttggttt ttttgtatcg acatcatcta tatcgcgtat tgtttttcct tccaattcta
    18001 atacttcatt ttctggagca aaatcgcccc tttttaaaac acgtttcttc atgttcttta
    18061 tacttctaat ctctttgttt acttcattta tatatttcaa ataattatca tacaaattaa
    18121 catattcttt agataaattc ataatgccat tcctccaacg ttattcctag atattttact
    18181 aaatctatta aatttattat atcctaatag tggtcatgtt ttctacatat taagtaattt
    18241 tttcaatttt aattttaaaa tgatatcttt taaagtgata cacaaatcat taataaaacc
    18301 tttattttcg tatactaaat ttaaataagc tgagagatat aatatacaaa tttacattat
    18361 aaactcccag cttgtaaaat tttctataaa tcttttgctt taaaccctct tccttttact
    18421 tctgtaacat catttacaga tataaaggcc tttttatcat acttcaaagc tatatctttt
    18481 atctttggta tctcatttga tgaaactatt gtatatataa tcttcttttt ttgatgagtg
    18541 tatgcacctt ctgcatctaa aaaagttaca cctctaacta aactcttcat tatatcttct
    18601 gctattaaat cgacttcatt tgacattacc ataacagatt tttggtaatt cattgcatct
    18661 ttaataaaac tcattgaata cgcatttaga aatagaccta taagagtgta taatgctaaa
    18721 tccaacccaa ataaaaaact tcctgtaagt actatcaagc catttattgc taaagcagtg
    18781 tctttcattt ctatattctt cttaattttt agtattgcag ctattatatc tgtaccacct
    18841 tgagaagacc tagttctaaa tacaactccg actcctaaac cagataatat accaccatat
    18901 acactttgaa gcaatatatc atgtataggt ataatattac ttatttcctg agttgctcct
    18961 aataataatg aaaatacaat catatttacc aaacttgaaa tacaaaactc tttttcaagg
    19021 tatatgaacc ctaagataaa tattggaata ttgattaaaa atgttaataa acctacattt
    19081 atatttgtca aatagtttat agatgttgct atacctgtag cacctccact aagtagatgt
    19141 gctggtctta agaacccatt tattcctata gatgtcatca acatcccaaa tagcattgtt
    19201 gaatatctta aaactggatg tttttgtagg gtattcttaa cttcactcat ttctttctcc
    19261 cttcaatctt tctaaatctt tagaatttta aaagcttact cttacatata ttattcctga
    19321 tgtaatagat aatatttcaa ataaattttt tttataattt cttgaactgt catctattac
    19381 atcatctatt atattatctt tttcatatat gtctagtgca atcttttata ttctaatctt
    19441 taaaaacgtt caaatcatct catttatgcc ttaaatgaaa ctttttctag cataatatga
    19501 tactgcttat tatttaactt gcaatatagt aaatttatag tatttttttc taaatatcta
    19561 ttttcgcagt aatttatttt attttagcaa attcaagttt agtaattgtc tctttataat
    19621 taattcctaa atgaaatttt tctttttata atttcagtac atgatttttt tatttcatta
    19681 tttgcatttt tttcgttcag tttttatttt aaattttttt tattttattt tgttttaagt
    19741 attcaagtta ctcgtcttaa ttttattttt tatataaaat tcattataat tggaattagt
    19801 attactgtaa ttgtaccaga gactccaata gaaacaccac tcatagctcc ttctatttct
    19861 ccaagttcaa aagcctttgt agttcctaaa gcatgtgcag atgttccaag agcaattcct
    19921 ttagcaactg gatgatttat attccccagt ttgaatactg tagaagcaac cataccacca
    19981 aaaatacctg taaaaattat ggctacaact gttattgatt ctacaccatt taaagaattt
    20041 gctaaagata ttcccattgg tgttgttata gattttggaa ttagtgaatt tattatactt
    20101 acatctgaat tagctatttt gccaattatc aatacagata caaaagaaat cactatacca
    20161 caacctattc ctacaataat ctcaaacata tgctttttaa ataattcaaa ttgtttataa
    20221 agtggaactg caagtactac tgtgacagga cctaaaaaga aatttagact atcagctcct
    20281 atcttgtaat tctcataagg gattttagat atgcttaaaa acaatattat tcctacgatt
    20341 gcaattaata aagggttaca tataggatta cttgtttttc gttgaatgta tcttcctaaa
    20401 ttgaaaaaaa ctattgttac tattatacca aatactggtg tctgtaatac attatacatt
    20461 tttttgtcct tcttccttct tttttatcat tttctccaca ataattccag tagtatacat
    20521 tactattagt gtacttaaac atatgactaa taaaagcact aatacatttt cccttattag
    20581 gtctaatgac tttataagtg atacacctgc tggaataaaa aatatagcca tattatcgag
    20641 gaaaaatcca cttacatttt ctatactact caagtctaaa actttaaatt gtagtagtag
    20701 aaataataat agcataccaa ctatactccc aggaataagt ataattcctt gtataaagga
    20761 acttatatac tctcctatta cccagataga taaaattatt accaattgat tgaaaacttt
    20821 cattttccac ctccaaaaat taaagagatt gcttcaaatc atatatttgt agttttatca
    20881 acaagcaaat atctttcttt aaaatagaaa gttataattt gacacaatct ctttttaaat
    20941 ttatttttat tttaatttaa taaaatacat acttggttgt ttcaaaaaac ttaactatat
    21001 aaacctattt ttcacttaaa atttctttta tttttgaatt gttatacata ctgtctggaa
    21061 cttcacttct caaactataa aaagtctgtt gtgcattttt taaatcgcca ttatcataat
    21121 aaagcatacc caactcgtaa tatgaatcat cataatactg gtcattttta gtataagtat
    21181 ttatatattt tttgtaatat tttatagctt catccttatt tcctaataat ttatttgtta
    21241 ttgctaattg atatattgac tcagaaatat attttttagt tttaccagaa gaaactacac
    21301 ttttaaaatt atctatggct ttttcatatt ctttgttttt ctttaaattt agagcttgat
    21361 aatataaatc ttcctcatca gctacagtta tctctctttc tggttcttta tttttaacct
    21421 catttaattc cttattcgtt ttttctaatt ttttactcgt ttctcctaac tttttatcag
    21481 tttcacttaa cttttcctct ttactactta tttggttatt caagttttct attctctttt
    21541 tgctttgaat caaatatatc cccataccta ttaaaacaca gccaactata atatatggca
    21601 aaatactttt ctttcttttt acttttttta tttcttcctt tttacttgta tcttcttttt
    21661 cttcttcatt tttttcaaat acaggattat ctttatctag ctctttaagt ttgtctaaat
    21721 atccttctct cttttttaca ttacctagtt tatcatataa caaagataaa attacatatg
    21781 gctctattag ttcaggttct tcttcaatta tgttctctaa aatatgtatt gactcataat
    21841 ttaattcttc atttataaat cttatagaat gattgtatct tcctaaaaaa gttttaaaat
    21901 cttcagatga aattagattt acatattttc tagatatatg attattttca taacataaag
    21961 cagtataaaa actctcaaat gatttagcaa aatcacattt taataatttt aaaagacctc
    22021 taagatttag tatatcaatg tctttaggat ttatctccag agctttatta accatattaa
    22081 tggcactagt tatataattt ttatatgcta aatccaatgc attgttatat cttttatatg
    22141 attctaattt taactcctta gacgttgtat ctgactttga atttaaagct ccttgcaagt
    22201 ttaatagagt ttcttcttta taatttgtag tcacataact ctgttttaat gcttttaatg
    22261 tttcatcacc gtcttcttct tctatattaa aaaaagtcaa atcactttta ttatcttcta
    22321 tcaaagatat atctaaaatt tttctaggca tgctttctat gtcttgtttt atatctttaa
    22381 ctaaaataat actcacctcc actctaaaat atatatttaa gataacatat ttaatatgtc
    22441 aagtcaaaac tatacatatt tttccaccat tctcataata aaaaaggtaa tcctcttctt
    22501 ttcataaaaa ggttacctct ttttcaatac tattaacttt taatgttgaa tttttttact
    22561 tttctataga gtgtagctat acctatattt aatgcctcag ctactttttt cataccttct
    22621 gtattacttc catatttctt tatggcattt tctatagctt ccctctcaag gtcatctata
    22681 gtcttaactt cttgtttata aagttcatct agtttaaaag atattaaatc agacatactc
    22741 cttacaaaag cagttaactc cttatctttt gaaagtatct tttctttttg ttcttcatta
    22801 aacgcagcta tacccaagat tccaagtata tgctctccca ttttaacggg agtacatata
    22861 tcagctaact ctttacaatt ttctctattt gcacatttat tacagttctc ttctatttct
    22921 ttatttataa cataggattt cccatctttt aaaacaagag agtacactga cttctctgat
    22981 attgtctctc ctattttacc tctgaatatt cctgtacctg ctatcctagt taaatctttt
    23041 gtaactaaag taacatctac atccaagaca ttagaaatat tctcgcaaat actttgaacg
    23101 taattcgtta tattatttaa attcaaagta atccctccta tttagattta tattttttat
    23161 tactctacat aaatcctatt cttgagaact ttaagcttgc ggttgtggtt gtgtctcatt
    23221 aggcttatta tcagttggtg gtgtttctgg tttttgttga ttatttgggt tttgattgtt
    23281 tgtattttga ttattcttat tttcgtctgt atttggtttt tcctctggtt ttggttccat
    23341 agtcccaact aatataatac cttccttctt aggataatat gaattagcta ttttttcagt
    23401 tttaattttt ttaccatttg catcattaaa tgttctatat gttgatacag aatatgcatt
    23461 tcttccactt ttgtctactt tttctttacc tttaggcatt gtagggtcat taactctttt
    23521 cattgtagtt tgtgaaacgc catctaattt tgttgatatt gttatatttt gtttatcttc
    23581 tgcacttcca tatatattgc aaacaatttg atttcctgaa acgtaatttt ttatgtaaac
    23641 tggatgcttt agattatttt taaataagaa atctattcca ctatctgcaa cagttgcatc
    23701 tcttcctttt ggaacataac ttgatggtat agtatgattt tttacattaa caatttctaa
    23761 acctgcgtat agcacagaat tgtataacgt agatgaaact tgacaaactc ctccaccata
    23821 atcttcttcc atctcacctt ccattattac aggtgcactt ttatatccat tttccttact
    23881 tcttttccct gtatgtttat taaatgagaa agtttctcct ggcataagta atacattact
    23941 tatattatct gttgctaatt ttatgtttat acttctattt atttgagaag ttttaaatgt
    24001 tgttgaatac gtacctaaat ttgtatctat attagcaagt tgctcttttt ttacttttgc
    24061 tggagtagct tttactacta actcattttt atagttacct tttttagttt gattatcaaa
    24121 attctccata ctcttagaaa tatccatttt aagaccatca gaatctggta taaccttgac
    24181 tttttcacca cttatatcaa gtgtagcatc ttttacatct acatctattt ctttagctat
    24241 cttttccatt tctgctttta atttattttt atcataattt atcgcaacta ctaagttgct
    24301 ttttttgcca aaatttgctg aaatagtttt tgatagattg ccaaagaaag cactctttct
    24361 atttaaatta tatgcgtttt ctactgtttt atccaggtca gtagataaat ttaaatcttt
    24421 gctctttact ttccagcttt tatcattgta attaaaatca acattctcta atttgtattt
    24481 tttagctaat tcctgttttg cttgtgattt agttaatttt ccaatattta ctccattaac
    24541 atatgtgttt tttgctattt tctccccttt aaattgcatg cacattatgc ctataaaaat
    24601 tattactact aatgctacag ctccaatagc tataactagc tttttgttca cattactcat
    24661 cccatacttc tccttacaat aaaattattt atatatccct attattatat tactacataa
    24721 tgcgccttaa aaccccaaaa actttttaat tatatattaa tttaatatta ttaattaata
    24781 ttcatttagt ccattttctg atatggcata tattttttca ttgtaaatat taatagcata
    24841 aacttctttg cctctaaata agcctttatt tactacagca taataataaa tatttttata
    24901 ttttccaaac aattcttcta aactcttttt actttttaca tatctatctg ctttctctat
    24961 atctaatatc tcaatctttt tatacttatt ttctttttca tcaagagtat tagttaacac
    25021 aaacttagct ttatcttttg taataccaaa ttgataactt ccatcttcta atattctaaa
    25081 gtatccatca ccataatcta ctttgtaatc ttttttacca tctttatatg aatataaatc
    25141 tttagttttt gcacttacca aaacttcatt tattaattta tcatactcat atacttcagc
    25201 ataatagtat ttattatttt ttatcatttg tggaagtgaa tttatagata gatttgcctt
    25261 gtctttgcca ctttttttag ctattacatt tataatgtca tcagatgtta aagtatacaa
    25321 tgataaattc tttttatatt ttttttctaa actctgtacc ttttcatcat ctgaataatc
    25381 gtcttttaaa tactcaatat attgtaatgc tctttcataa tctccagaag tatttgcctc
    25441 ttcagctcta cttatatagt aatcatacat ttcttcaata cattgttctt tcccattttg
    25501 agccatactg taatactttt catcttcttt taatacttta ttatactttt caatagcttc
    25561 ataatactta tatgcttttt ggtcttttac tgctgagtca taaacatctc tagactcttt
    25621 gattgtttct atattctgtt tgtatacatc taggttacta cccataccat ttaatattga
    25681 aacagtactt atgtagttaa tggcaatatc ataagtagta ttttcttctt tatacatttc
    25741 tctaactcta tcagcttgag ctaataagtc atccacatct atacttattt tgtttaattc
    25801 tttcacggta tttattaatc caataaaact ttcttgactt atgtctccat ctaaatattt
    25861 atcaccacta tttaatagca atttatttat ttttttagaa acagacttat taaacctttc
    25921 catttttgaa tcagaaaact ttttttctat acttgtgtag taatcaacag ctatagaata
    25981 ctgtcttttc tctataatct ttaacaactc attagcttca tttcctacac taaactcgtt
    26041 actacttaag aaaaatacta ttatcaaagc aattacacct aatgttgata atgaaatcgc
    26101 aactatagat ttcctttttt catcaataaa atctacagcc ttctcaaata aatctctaaa
    26161 attcattata gttccctctc catttaataa tctttatatt ttatggtaaa ctaaatttct
    26221 tattggttat tatatcataa ttcttgaaaa aaaataaaaa cacaagttaa atcgtgtttt
    26281 tattttacct atcaatcatt attttataca taaatatttc ctttacataa attatgatat
    26341 ctttcttttt atatccctag aatgactctt ttgtttttta ttattactga cagttttcgt
    26401 tcttgtcttg tgtatagctt tgctatcaaa tatttttcta gcattgcata aaacaattcc
    26461 aattgttaaa caaactgtaa gcacacttcc catcaaaaag taataatcca atcctatatg
    26521 tactcccata actgaattta ttaacactaa tgcaaaagtc attgcaaaac atatcatcat
    26581 ggagataact aaaattctta gaattttttt caatccactc acctcgtata atcactaaaa
    26641 ttgacactac cccttatcca ttactatacc aaattagtaa aaaaatagca attactattg
    26701 tcatttatat taggaaaata ttaaatcttt ttaataaata tctttttttg acataaggat
    26761 tacttaactt ttaataaaat agtaacacta atatgtatta attaatagaa taaaataaat
    26821 gataatttat tgaggggtga tttacatgcc aaatttgcca agtttagggt caaaggctcc
    26881 tgattttaaa gccaatacaa caaatggtcc tattagactc tctgactata agggtaattg
    26941 gattgtttta ttttcacatc ctggtgattt tacaccagtt tgtactacag aatttttatg
    27001 ttttgctaaa tattatgacg aatttaaaaa aagaaataca gaactaattg gtctaagtgt
    27061 tgatagtaac agttcacatt tagcttggat gtataatatt tctttactta caggtgtaga
    27121 aattccattt cctattatag aagatagaga tatgagaatt gccaagttat acggcatgat
    27181 atcaaaacca atgagtgata catcaactgt tcgctctgta tttattatag ataataatca
    27241 aattctaaga acgattcttt attatccact aactacagga agaaatattc cagaaatact
    27301 tagaatagta gatgcacttc agactagtga tagagataat atagttactc ctgcaaactg
    27361 gtttcctgga atgccagtga ttttacctta tcctaaaaac tataaggaat taaaaaatag
    27421 agttaacagt tgtaataaga aatattcatg tatggactgg tacttatgtt ttgtaccaga
    27481 taattataat gatgaagaag tgagcaagaa aattgataat acttgtagct ggaaaaaaga
    27541 acatactaaa aacattgaaa atgaatgtaa ctgtgaacat gaacatcatg actacctgaa
    27601 caaagctctt gactgtaaac aagaacacaa gactgatatt aaagatgatt gcaatcatga
    27661 gaaaaaacat actaaaaata ctaacaaagt tcacaactcc aaacaagata agtttaaaga
    27721 taagtcttgt gatgaaatga attttaacta tgataaagat gaatcttgtg acaaaataaa
    27781 ttctagctat aacaaagaag atagtagtta tgaagatttc tataaacata attataaaaa
    27841 ctacgattat actagcgaaa aaaatactaa aaaaatagct atgaaaactt taaaagattc
    27901 taaaaaatta gttagaccac aaataacaga cccatacaat ccaatagttg aaaatgcaaa
    27961 ctgtccagat ataaatccaa ttgtagcaga atatgttctt ggaaatccaa ctaatgtaga
    28021 tgctcaacta ttagatgcag ttatatttgc ttttgctgag atagaccagt ctggaaattt
    28081 gtttattcct tatcctagat ttttaaatca attacttgct cttaaaggtg aaaaacctag
    28141 cttaaaagta attgtagcta ttggaggttg gggagctgaa ggattctctg atgcagcatt
    28201 aacacctaca tctagatata attttgcaag acaggtcaat cagatgataa atgaatatgc
    28261 tttagatgga atagatatag actgggaata tcctggaagt agtgcatctg gaataacatc
    28321 aagacctcaa gatagagaaa actttacact cttactaact gccataagag atgttatagg
    28381 agatgataaa tggcttagtg tagctggaac aggagataga ggatatataa attcaagtgc
    28441 tgaaatagat aaaatagctc ctataataga ttattttaat cttatgagtt atgattttac
    28501 agcaggtgaa acaggcccaa atggtagaaa acatcaagca aatctttttg attcagactt
    28561 atctttgcca ggatatagtg ttgatgcaat ggtaagaaat cttgagaatg ctggaatgcc
    28621 ttctgaaaaa atccttcttg gtataccatt ttatggaaga ttaggtgcta ctataacaag
    28681 aacttatgat gagcttagaa gggattatat aaataaaaat ggatatgaat atagatttga
    28741 taatactgct caagttccgt atttagttaa ggatggagat tttgcaatgt catatgatga
    28801 tgctttatca atattcttaa aaactcaata tgttcttaga aattgtctag gtggtgtatt
    28861 ctcatggaca tcaacttatg accaagcaaa tatactggct agaaccatgt ctattggtat
    28921 aaatgaccct gaagtattaa aagaagaact tgaaggtatt tatgggcaat tctaatagaa
    28981 tgtagtaaaa ttttatagtt atataaaaag cagatttta
//
LOCUS       DAYAOX010000022        28943 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.022, whole genome shotgun sequence.
ACCESSION   DAYAOX010000022 DAYAOX010000000
VERSION     DAYAOX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.022"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..420)
                     /locus_tag="ACGI57_000839"
     CDS             complement(<1..420)
                     /locus_tag="ACGI57_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715925.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIK"
     gene            complement(623..1603)
                     /locus_tag="ACGI57_000840"
     CDS             complement(623..1603)
                     /locus_tag="ACGI57_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9715926.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDRPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
     gene            complement(1808..2662)
                     /locus_tag="ACGI57_000841"
     CDS             complement(1808..2662)
                     /locus_tag="ACGI57_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HFG9715927.1"
                     /translation="MKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKEVP
                     SKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDIEE
                     IEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCDKI
                     IKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLNTI
                     KEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEEEE
                     EKYRRIFK"
     gene            complement(2985..4313)
                     /locus_tag="ACGI57_000842"
     CDS             complement(2985..4313)
                     /locus_tag="ACGI57_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFG9715928.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(4461..4790)
                     /locus_tag="ACGI57_000843"
     CDS             complement(4461..4790)
                     /locus_tag="ACGI57_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9715929.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(4814..6103)
                     /locus_tag="ACGI57_000844"
     CDS             complement(4814..6103)
                     /locus_tag="ACGI57_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9715930.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(6119..6445)
                     /locus_tag="ACGI57_000845"
     CDS             complement(6119..6445)
                     /locus_tag="ACGI57_000845"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9715931.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(6580..7209)
                     /locus_tag="ACGI57_000846"
     CDS             complement(6580..7209)
                     /locus_tag="ACGI57_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715932.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(7424..10081)
                     /locus_tag="ACGI57_000847"
     CDS             complement(7424..10081)
                     /locus_tag="ACGI57_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9715933.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(10440..11384)
                     /locus_tag="ACGI57_000848"
     CDS             complement(10440..11384)
                     /locus_tag="ACGI57_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9715934.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIDVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(11377..12402)
                     /locus_tag="ACGI57_000849"
     CDS             complement(11377..12402)
                     /locus_tag="ACGI57_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9715935.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(12477..14045)
                     /locus_tag="ACGI57_000850"
     CDS             complement(12477..14045)
                     /locus_tag="ACGI57_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9715936.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(14158..15069)
                     /locus_tag="ACGI57_000851"
     CDS             complement(14158..15069)
                     /locus_tag="ACGI57_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9715937.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(15053..16039)
                     /locus_tag="ACGI57_000852"
     CDS             complement(15053..16039)
                     /locus_tag="ACGI57_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9715938.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            16357..16800
                     /locus_tag="ACGI57_000853"
     CDS             16357..16800
                     /locus_tag="ACGI57_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9715939.1"
                     /translation="MILLKSNYDCFRIAMLLKELYSKTMYTVEENFKENGLTHQQIIV
                     IKLIAHNQELTISQLCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTT
                     GFEFATNFKIKMQESFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            16803..17201
                     /locus_tag="ACGI57_000854"
     CDS             16803..17201
                     /locus_tag="ACGI57_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsmA family protein"
                     /protein_id="HFG9715940.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(17651..17875)
                     /locus_tag="ACGI57_000855"
     CDS             complement(17651..17875)
                     /locus_tag="ACGI57_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HFG9715941.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGVTMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(17910..19277)
                     /locus_tag="ACGI57_000856"
     CDS             complement(17910..19277)
                     /locus_tag="ACGI57_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9715942.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(19361..20896)
                     /locus_tag="ACGI57_000857"
     CDS             complement(19361..20896)
                     /locus_tag="ACGI57_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6179 domain-containing protein"
                     /protein_id="HFG9715943.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EVSLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(20919..21353)
                     /locus_tag="ACGI57_000858"
     CDS             complement(20919..21353)
                     /locus_tag="ACGI57_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6323 family protein"
                     /protein_id="HFG9715944.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(21613..22524)
                     /locus_tag="ACGI57_000859"
     CDS             complement(21613..22524)
                     /locus_tag="ACGI57_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HFG9715945.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            22820..23173
                     /locus_tag="ACGI57_000860"
     CDS             22820..23173
                     /locus_tag="ACGI57_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFG9715946.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            complement(23392..24333)
                     /locus_tag="ACGI57_000861"
     CDS             complement(23392..24333)
                     /locus_tag="ACGI57_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HFG9715947.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(24434..25669)
                     /locus_tag="ACGI57_000862"
     CDS             complement(24434..25669)
                     /locus_tag="ACGI57_000862"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFG9715948.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            complement(25713..26567)
                     /locus_tag="ACGI57_000863"
     CDS             complement(25713..26567)
                     /locus_tag="ACGI57_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715949.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            complement(26695..27870)
                     /locus_tag="ACGI57_000864"
     CDS             complement(26695..27870)
                     /locus_tag="ACGI57_000864"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HFG9715950.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            complement(27969..28853)
                     /locus_tag="ACGI57_000865"
     CDS             complement(27969..28853)
                     /locus_tag="ACGI57_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFG9715951.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
BASE COUNT         9281 a         5151 c         2927 g        11584 t
ORIGIN      
        1 tttaatgtag attaacatat gatatgtttt aaacatcaat ccccttgctt tcttatatat
       61 atttggcaac acatatgagt ttaataaatt atatacaatg ttgctttctt cttgtatttt
      121 ttcaaccaac aagttattta taaaattacc tttctcattt tcaataatct cactccaatt
      181 tatcttaact tttaatatat atcttcccac taggtataca cactctacaa taaataataa
      241 aaatacaatg cagtcagtac ctgttgtctt atataaatta cccgatacta atggctttgc
      301 gaagaaatta taaaaattca aagtatcacc cgtataatca aaaagtatta atagcactcc
      361 tagaactctc aatactttaa tattcaacat gtttttatct ttattatttt tgtttttcat
      421 atatactacc tctcttttct ttagatttga ttgtaatcta atactaactc ttttatttga
      481 ggtataaatg aatttaattg gtagttattt tcatttttac tagaaattaa ttcacattac
      541 taaacaaaac ttatatttaa acataaaaat agcctatcta ttttttaata aatacaaaaa
      601 taaataagct actaatattc aattaacttt tatcacttaa aacattacta tctataaatt
      661 taaatatttt attgaagtat actgtactat catgttcaaa tccatcaaca tgaccaatac
      721 cttctataag ccataggtct tttttatcat gtttaactaa atcatacatt ttcttgctat
      781 tttgataatt acaaacagta tcttttgttc catgtataaa cagtatgggt acattagatt
      841 tctcaactga ctcatatggt tttacatcac tgtaccaaaa tccagattta aacttattat
      901 ataaatctcc acaagtcacc acataattga ttaatatatc tggtattcgt ttttctagta
      961 ctcccattct aattgcatct ttcatttcag aataaggaga atccagtata taaaaatcga
     1021 ccttatcatc ttttgaattt atctctgaat gcatagcagc tgtccctgcc cccatggaaa
     1081 atccatgcac tcctaatctt ccttcaggaa atttattttt tacaaattta actagactat
     1141 ctaaatcata cctttcataa agaccaaatg tgtaattatc tccaccacta ttaccagtat
     1201 ttctttgatt ataaactaat acgttataac ctttatctaa atatctatat gctaccttta
     1261 acatttcata gtaactactt ccaattccat gaacaagtat catagtatct cttgtcttta
     1321 tatttgaagt tataaaaaga ctttcaagct tgtaattatt agaactttgt atcattaatt
     1381 cagatgtctt gtagtttttt aatttctcca gtggtctatc ttttctctca gaaaatattt
     1441 ttactatatc ttcgtttttt attaactgct ctaaaccaac tgaactatta tacactaatt
     1501 tgccaaatat ataagataca attaatagga ttaatagaaa cacaattgat ataattataa
     1561 atttatttct taaactaata caatttttaa aactattttt catatttatt taccatacaa
     1621 ttttcctata ttatattttc taagtaatag cataattgat tatactattc ttataatttc
     1681 tattatacct acttatttat taaaaataca aattatattt tattaatttt tactacttgt
     1741 aaattattat cttatacaat gaatttgtat tatataataa gtatttttag gattctgtta
     1801 cataatatta tttaaatatt cttctatatt tctcttcttc ttcttcagta aacacattaa
     1861 atacaacttt taaatttttt tcaggatttt cttttagcca tagtctaact ctacttacag
     1921 caagattagc tgcctctttt tttggataac caaatacacc tgtagaaatt ccacaaaaaa
     1981 caatattttt tatttcatca atttctttta ttgtatttaa acatgattta taacaatgaa
     2041 gcagttgctt ttcttgctct ttacttggct gaccaccaga tacaataggt ccaacagtat
     2101 gtactacaaa ttttgcaggt aaacaataac ctctagttat cttagcatct ccagtatatt
     2161 ctaaatgccc ttgttttttt attattttgt cacaatcttc tctaagtcta ggacctgcgc
     2221 atgaatgtat ctcattatcc acgcatagat gtaatggttg taaacaccct aataacttgt
     2281 tatttgcagc atttactatt gcatctgctc ttaaattcgt tatattacct ctccatatgg
     2341 caatcccttc ctctatttct tctatatcat ttacatctac taactctctt tcttcacatt
     2401 caatcaacaa taaattatgt aacatattaa gtatctctgg agcaatttct tttggtggat
     2461 atacattcaa aagtcctcta agtatatctc taggcttaat ttcatcattt gataaattgt
     2521 atattttaga aggaacttct tttgaaaaat atgcaattaa agtgtttata ttttttcttc
     2581 tttgttcgtc agttagaggc ttgatagttt tatcaaaatc ctcaaataaa tttacatcat
     2641 cagcataatc tctccacttc atagccatct ctccattctt gtattttaga cttaaaaata
     2701 attatgtatt tatgtacata tttatttatt tctataaaca cctacataaa tcctgttaaa
     2761 agtgtaaatt ttttattatg caacttctct ggttatttct attgattgat agtaaattat
     2821 tgtacctaaa tttaatccaa ctaacaagta actaaaccta ctttcctaac tgcaaaatac
     2881 actatgtttg ccacttgtat ctcatttgta aataaataaa gtccctacaa atacattaac
     2941 taggctatgt actaaactgt aggaacttta aaaatatatt attcttaatt aagttttggc
     3001 caataatttt tatttgcttc tattaaatca tctagtaatt cttttgcaac tgttgcacta
     3061 ggaactgtct tagatagcat aagtgcttgc cacaatttat tataagactt ctcaatccaa
     3121 gcatcaacta ctaatttttc aactgctaat tgttgttcta ttaaaccttt ttggaatgtt
     3181 ggtattttcc ctattgataa tggttcatac ccgtcttttc caactataca tggaatttca
     3241 accatagcat catcttgtat attttctata gctccattat tttgaactat caataacata
     3301 cgctcctgtg tattaaaagc tagagctgta gctaaatcca ctataaactc tgcatgtatt
     3361 cctatcttaa gcccactgcc cttagaatgt ccttgctttt ctatcaatgc acatgaacca
     3421 aaaacttctt tttctcttcc atcaataact tcattggcac gagtataatt tacatcagaa
     3481 tgatttacaa cataatctgg aaatagatag tactttaagt aactacttgg tattgtagtt
     3541 aaatctgttt ttactatttc tttagtaaat aaattagttt caaaccaact atctttcttg
     3601 tcatttttca ttccttcttc accatttata aaaccatatt cttttatatg ttctactaat
     3661 tttggtaaaa gttcatttcc ttttttatct ttaattgaag tataccaacc aaagtggttt
     3721 aatccaaagt atcttatatc catatgttta cgagatttga gtcctgcaat ccttgctata
     3781 ttatgttcca tacctattgg catatcacaa atatttatta ccctagaatt aggtctcaac
     3841 cttctaacag cttccgctat tattgatgct ggattagaat aatttaacat ccaacaattt
     3901 ggagaatact tttccatata atctatgttt tcaagaacac cttctataga cctcattcca
     3961 taagctattc cacctggtcc acaagtttct tgaccaacta ccccatgttt tagaggtatc
     4021 ttttcatcta gttctctcat ttcaagtttt ccaactctta tatgagctaa acaaaaatct
     4081 acatctgtat aagcctcttt agggcatgtt gttgctaaat actctatatt agggtccttt
     4141 tcctttatta gtatttcaca agctttagct accttatttt gtctttcttc atcattatca
     4201 tataatttga ttgctcttaa tggtaaccta tctaagttat ctagcaacat taatatgatt
     4261 tctggggtat atgtacttcc tcctccagct attactactg aaaatttttc cataataata
     4321 tctccttttc tatttaaaaa tttactgtat ttataaataa atttataaat actagtactt
     4381 taaagtaaaa gggagttcat ttattaaatc tattgattta ataaactata gccaaacttc
     4441 aagcactatt tacaagaaat ttaattctta ttaattcttg ggtccatcat ctcaaatata
     4501 tctacaagtt cagatactaa agaatgagca aataatgcat ttgaataatg gtcttcagag
     4561 tgcacaacta aaaaatcagt cccactatct tcactattca tagcatttga tagtaattct
     4621 aaatgatgct ttgatgctgt ttgtattttc tctgaggctg aatttaaaag gtgttttgct
     4681 ttattgtagt cacctttttt tatttcctta agagacatca ttacatcact tttagcatct
     4741 cctgcttctg atattatact aaatactttt tcttccattt caggtaccat atccttctcc
     4801 ttctaatgac ttgttaaaaa tcaaagctat catcatctaa tgttgtagct aattctgcat
     4861 ttttatttaa ttcttccttc tccattatct taaagaatgg ataatatatc aaggtcataa
     4921 gtattatact tgcaaatact aatacgattg ccttccaatc taatgtcatc aaaaatgctg
     4981 caacccctgg aggtaaaaag cctggtggtt ctgcaatagg taatcctact aatcctactt
     5041 ttaatgccat aaaattaagt gctccaacca atacaggtcc aaatacaaat ggtacaaaca
     5101 tatatgggtt cataactatt ggtgttccaa atattaccgg ctcatttatt ccaaatagtg
     5161 atggtaaaat cacaactttt cccatactat taaaactctt acacttactt cttgacataa
     5221 ggtacactaa tcccaaagtt atccctgaac cagttacttg caagaaacca tagtaaaaac
     5281 ctattgtaaa cacatatttt gggtctcctc ctgctgctat taacgttgca ttttctgcca
     5341 tatatgtagc tgctattggc atccatatac ttgaagttat tgctgctcca tgaagcccaa
     5401 agaaccataa taattgagtt ataaatagta ttatagttac tgcaaaaaca ttatctacac
     5461 tgcttatagc tggtgctaag aaattcatta taatagctgg taatggttga cctgagacat
     5521 ttttagttat tgctgctatt gctgtaaata acattataca tactatcatt ggagttaagt
     5581 ttctaaaact tttatttact acactaggta cttcttttgg aaatctaatt acgagatttt
     5641 tatcattcat aaacttagta atatcaacta ctaaaagtgc tactaatata cttacaaata
     5701 aaccttgtgc atctaaataa cttgtatcta atacaccatc tactacttgt ccacatacaa
     5761 caagttgtgc aacaactgct gtcataccta tttctacatg attaagatta tattttttag
     5821 ctagtgcctg agctataaaa aatgctgaat atatagataa taaactcata gtaaatttat
     5881 tagggataag taatgtatcc atattttcag ataataatgt ttttgcactt ccttcaggta
     5941 acatattgtt taatgccata aataacatac ttaaagaacc aacaattatt attggggtaa
     6001 tgcttatcat accttctttt atagcttgaa gatgcctttg tttttcaatc ttttgtgcta
     6061 ttggaagaag aaatcttctt aaattatttt gaagtgcttc catttttatt ccccccaatt
     6121 atctattttt atataattcg attgccgact tcataacaga aggagcatcc atacgaccat
     6181 aatgcttagt atctattaaa ccaactgcta ttttgtaatt cttacaagct tcaactacat
     6241 catcttcctt aaacctaatt tgtggtgcta caagtactac atcggtatca ttagcttcaa
     6301 tgtatgattt taaattttca aatgatgttg cctctatatt tatagtttta gtttttaact
     6361 tttcatgctc actacaaaaa cttttcatct tttccacaag tacacctgtt gaagctccat
     6421 ttgcacatac taataaaata ttcataatta ccctcctaca atatctaacc tcttttattt
     6481 tctaattttt tatcagccaa ttcttactct aattcctatc agtttttact ttaattctta
     6541 ttattttatt ttaattctta ttaatattta ctctaattct tagtactgcc aatgattaaa
     6601 cttgaaagcc gtatctataa cttcttcttg cttatatttt tctaatattt ttaagtatct
     6661 ttctttataa tcattatcta cttctttttc tggtattact atactagctt catttaaaaa
     6721 gtggaatgaa tcccttccca tttctaatcc tctatatgta tgcttgtcaa gtgccacgtc
     6781 cataacttct ggaactttac ccattgcaaa tccttttata attatatttt ttaataaatc
     6841 acttgatcta tctttctcac atgcacaaag atatcttatt gcatgtatga aaaataaagg
     6901 tctgtctcca tctgcatatg aatattcctt tcttacttga tttaaattat ttatgattat
     6961 tacagctgat gggtccccca ttcctatatc ttcaactgat attgtaagta tccttctcca
     7021 tagcttatct tctaattctg gtgatgatat atatagttca taagcaaatt cacaagcttc
     7081 ttccaccata tttcttctta tagctttttg aaatgacgat attatttcat ctgcactaaa
     7141 tccgtttctt gtagtcattc ttgcccatgg gtcatttgcc tttttatctc ttaatatttt
     7201 atcttgcata ttatttactc ctttttattt aaattttaat tatttatatt actcatttaa
     7261 taatataagc aagtattatg ccagtttttt tagacacttt aaaacactgt tatttcaatc
     7321 atttttatca tcaaaactta gatactttgt ctaagtatat ttatatgtgt ctaatattaa
     7381 tattatttta gactaaaaaa aattaccaaa taagttttta tactcattgg taatcttttt
     7441 cgattatttc tgcatattct aatattaata atatttgata gcactctttt tcattaattt
     7501 ctatattata ttttttatct aattgagata ctatatcttt tattttgctt aggctcttgt
     7561 gataatcaag tgttttttct ttatcaagct tttcaatttc tctagtattt ccaatcaatc
     7621 catctaccat gaaagctaaa tgcatcataa gtccaattac tttatcgtat ttcttttcta
     7681 tattaaatac taaaaacaat tcatcagaca catattttat atctttaaat atcctattat
     7741 aattagagct ttttaaggat gtctttaaac cttcaactac atcatcataa cctatactta
     7801 tctttataat attttttaat ttattgatat tttcatcctt atctatatct tttttgctaa
     7861 tatagtcaat tcctctaata ttaattcttt gttcattgat tactgcaaca atattactac
     7921 tttctttaag tttcgtaata tttaaactta aatctttttc tgttttatat gggacagata
     7981 taatatctat tccatctaag tacctactca tcttttcttc taatattttt cttgttttat
     8041 tatctacacc ttctgaaaca tgtgatgcta taataattgt tttagacaga ttttctttat
     8101 tttgaatttc attagttcca aaatagtact ttctcgtgtt agttatagag ttgtatacgt
     8161 catttaaagg taatcccata ttggccttct gaactgcttc taacacagtt aatgttgtca
     8221 ccataggaat tactcttact ccacactttt tttctttctt tataatttca tcaaaagagt
     8281 ttaatgaacc catatctgta aatagtaata catctttatt gtctatatta ttaattaaat
     8341 ttttaaaatc tacaataact tcatcatagc ttttatctaa cttcatatcg aaagaaatag
     8401 cactgcactt tccaataata tcaccaactg cttttaccat actacttgct gtagtttctc
     8461 catgcatagc aactataata ttaatgttat tcactatttt attttgttca tcttgtttag
     8521 cttgtactat gaataatgta atatatgcta cagtttctat agataaaaat aaatcaaatt
     8581 cattttcaat tattccaact atttttagtg ctgttttaaa ttcacttctg ttatttcttc
     8641 taatttgatt caaatctatg ttgtttgtag agctactttt atccaatacg ctttttatat
     8701 aaattgccat tgacttaaat attgattcac ttatgtctat ttcttcatct tctttaataa
     8761 tttgtcttat tgattcacac atgcttttaa acttaacatt attaaataga gaagcatctg
     8821 ctatattcat ttctacagat ttattaagat tagtatttac tgcattaata atatttatat
     8881 cattgtagtt aataacttct tcttctccta atgacgtaaa tacaaaatat tctattccat
     8941 gtgttataac ttcatctgct ttaatagact tctctctata atcatcactc acttcattta
     9001 tagatttatc aaaatatttt ttctctatat aaactggttc tttattatct cttttatatc
     9061 ttaggtatcc atttgcaaca gctaatttta tatcattttg aagttgacct acattatttt
     9121 tacaatcata aagtaatagt gatttaatta tttctttatg tacttttatt gaaatagaaa
     9181 tactttttga ttcttccttg aaaaaatttt tgactaattc cattctttca atcattgtcc
     9241 tatctctcag tgatggtatt tctattttca ttggtattct tctattgaat gtatctaata
     9301 gtgttgatgt cggattttct gtagtagcac atattattaa taccttagac tctctttctt
     9361 tcgttgcatc tcctagtctt ttaaatgttc ccttatctat aaaagtaaac aacatttctt
     9421 gaccttctgc tggtagtcta tgaatttcat ctaaaaatag tatccctcca tttgcaattt
     9481 ccaaaagacc ttttctggaa gattctgcac ctgtataagc tccttgttct accccaaaaa
     9541 tatgtcccat aagtaattgt gcattattag cataatcagc acaattaaat gttacaaaag
     9601 gagctttatt ttttctaatt ccactatcta tagaataatc gtacatatat ctagcaatca
     9661 tactctttcc tactcctgtt tctccataga taatagtatg aagtccattt gtaggataga
     9721 gcattgcgac cttgcatttt tctattatag gtcttaaact atgttcttta cctattagtg
     9781 tttcaaaact atcttttttt attttgtttt ctacttttgt tccttttttt atgccaagca
     9841 actcactttg tgtatcaagc aattcattct tgttaaggtt aagatttgca gggatataat
     9901 acacaggtct tccttttatc ttcgtaacca taccctcatt gaataattgg tttaaatatg
     9961 tacttatatt gtttcttgca attcctgtct tttctgataa ttggttagct gtaatccctt
    10021 tttcattttc cctatatgat gattctaaaa ctttcattac tttatctttt ttatatatca
    10081 ttacaagctc ccctacatta tagtatttta ttaactaaaa cccctataca cactttattt
    10141 tttaataaac tcttattctt taactaaggt tataatttat caaactttcc ttactttaaa
    10201 ttatagaaaa ttattgtaga aaaaataaga ttattgctca ttttttctac attactattt
    10261 ttaattttat agtactttct taacaacata ctttacttgt ttttaatttt attatatttg
    10321 aatttattat atttgaaaag tagaaataaa aaaccctatt ttggagtagt tttaaaattt
    10381 ctccaaaaat agggttttag tttgtatttc aaataaatac ctgttcaagt atttttatac
    10441 taaacaactt ttaaattttg ttgatattta tttatttctg atttagaaga atatacaaaa
    10501 tgtccatctg aaacatcaat ccaacttaat gacttttcat ttgaacaatc atatccatta
    10561 gaattatact ctattctatt tcttgactta gcatatctag ggtcaggaac aggaattgca
    10621 gaaagtagtg attttgtata tggatgaata ggattattat acaattcatt tgaatctgcc
    10681 aattctacta attttccatt atacataact cctatacggt cactaatatg tctcaccata
    10741 gataaatcat gagctataaa taaacaagtt aaattctttt cttgttgcaa ttttttaaat
    10801 aaatttacta tttgtgcttg tatagacaca tccaaagaag ctataggctc atcagctatt
    10861 ataaattctg ggtctactga aagagctcgt gctatgccaa tacgctgtct ttgtccacct
    10921 gaaaaatcag atggatactt atttgcataa cttctatcaa gtcctactag ctttagcatc
    10981 tcatatactt tattcattct atctgtttta tttttacaga taccttgaat ttttagtggc
    11041 tctgaaatta tatctgcaat tgtcatacgt ggatttaatg atgaagtaga atcttgaaaa
    11101 ataatctgca tacttttgtt tacatctttt tttataaatt tgtacgtttt tttatctgat
    11161 atgcaatttc ctttgtaaat tacttctcca ccagtagatt catgtagtct tattatagtt
    11221 ttaccagttg tagattttcc tgaacccgac tctcctacca atccaaatat ctcaccctta
    11281 tatatatcaa aacttatatc atcaactgct tttacagtag tacttttgtc caaatgaaaa
    11341 tattgcttta agttttttat ttccagtatt ttttctctat tcattagaaa tcacccttcc
    11401 acaatttact ctaactggta catctacttc aggtgcatct gggtgcaata accaagttgc
    11461 tgcactatgt gtatcattaa ttttaaacat tggtggctct ttttcataat ctatcttaag
    11521 tgcattttta ttccttattg caaaagcatc tccttttggt gggttcaata aatttggagg
    11581 cattccaggt atattatata aatagtcatc ctgcgaatct aaagtaggta atgaacccaa
    11641 caacccccaa gtatatggat gtctagggtc ataaaatata tcctctacag ttcctatttc
    11701 tacaatcttt cctgcataca taactgctat tctatcagct atagttgcaa ctactcctaa
    11761 atcatgagtt ataaatatta ttgacaaacc tattttatgt tgtaaatcct ttattaaatc
    11821 aattatttga gcttgtatag ttacatctaa agcagttgta ggttcatcag caatcaatac
    11881 atctggatta caagcaagtg ctatagctat tactatacgt tgtctcattc cacctgaaaa
    11941 atgatgtgga aattgtttaa atcttttctc aggattatca attccaacca attcaataag
    12001 ctctatggct ctcttttttg cctcactttt acttatacct tgatgaatga ttacagcttc
    12061 tgcaatttgc tttcctattg atatagttgg attgagagat gtcatagggt cttgaaatat
    12121 cattgatata ttctttcctc ttatatcttc catctctttt tcagacttct taaccaaatc
    12181 acttgattta agtaaaacct ctccattttt aatatatcca ttgtatggga gtatcctcat
    12241 caaactctta cacagtacag atttcccaca accagattct ccaactatag ccaatgtctc
    12301 accttttttt agattaaaag atacatttct tacagcctga acttctcctt cttctacttt
    12361 aaaacttaca gatagattat taacctccaa taaatgttcc aaaaaccatc aacccctatg
    12421 ctattcaaac cttagggata tatttttatc cctaaggttt ctaattaatt tcagtattat
    12481 tcaattgtcc agtcagctat gttccagaat atacctacac catgatgacc taaaactgta
    12541 tcaggtgtta atccttttat gtttggtttt ccaacatata ttgcatctat ataagcaata
    12601 aatgtataag gcatatcctt agtcatttct acttggaatt gtttatataa ctttaatttt
    12661 tcatctttat cttctgtttc tcttgctttt tgaagtattt tatctatagt aggattagag
    12721 taagcactgt aatttgcacc tttatctgtt ccaaatactt tatatgtatg gtcatctggg
    12781 tcaaatggac ttccccaacc tattaaatga gcatcttgat tagcccaatc tgtttcagtt
    12841 acaacaacag ctttagcatc aacaccaatt tcttttaact gttgtgcaca tattttagcc
    12901 atatcaactc taacttggtc actctcacct gctgttatct cgaaagctaa cttagtacct
    12961 tctttttcat agattccatc agaacctaat ttccaaccta gcttttcaat ttcttgttta
    13021 gctttttctg gattatattc aaacttttct atatcaggat tattatatgg tcccatttgt
    13081 aaaggtgaat atgctggtac accatgtcct aataatacac tgtcaactat tgcttttcta
    13141 tctattgcat agcttaaagc atttggtaat cctttagctt ttttatcttt aaagaattta
    13201 gaattaaaat tgtataatat acctctatag tcggctgttt tcattatatt tactttaaaa
    13261 ttcttttcgt ctttttcaaa gttagacata tctttaggag ttatttgagc taggtctaat
    13321 tctcctgatt ttaattgcat tgccttagcc ttatcatctg gaacaatctt aaagactact
    13381 ttatctaatc ctggttcttt tacaaaataa tcactattct taacaagtgt tatactttga
    13441 cctttgtccc atttttcaag tttaaatggt ccagtaccta ttggtttttg attaaattca
    13501 tcagtagcta tatccttacc ttccaaagca tgttttggta atactcctac tgttaaataa
    13561 tcaagcattg ctgtatttgg agcttttaaa gtaattttta tagtattatc attaaccaca
    13621 tcaatttttg ttatatcttc ataatttgat gctatctcag aagcattatc tggattcatt
    13681 atagtttcta ttgtaaactt aacatcattt gctgtaaatt tttctccatc atgccactta
    13741 acatcatctc ttaaattaaa agtatatgta tttgttgcct catcaaattt ccagtccttt
    13801 gctagacaag gaaccacctt attattttca tcatgagctg ttaatccatt aaatattaac
    13861 gaatttattt ctccatgttc atatagtgca ggatttatac ttgtatagtc attacttcca
    13921 taaacaagca cctttccatc tttaggcgta tctgccttct tatctttgtc tcctccacta
    13981 gaacaacctg ccattagact aagtatcatt actaaactta aaacttttaa cttctttaat
    14041 ttcatgatgc tccccctttt gctttttata aaatattttt caaagctttg accccattta
    14101 tttaaataga tatttcaaat acctcagtat ttgcttattt aatactcaaa tgctgattta
    14161 taaattacta gattttttat tattattctt tcttatataa ttacctatat ttgttataca
    14221 caccaatgtg gtaaccaaaa atatacccgg aatcagtata atccaccatc tatttgaaag
    14281 taatgcttcc tctgacaaag ataacatact tccccaagaa acaatttcta ttggtaaacc
    14341 aattcctaaa aagctaagtg tcgattcagt accaatagct gcgcctatgt ttgttacaac
    14401 catgaacatt attgatgcca caaaatttgg aagtaaatgt tgctttaata catagaagaa
    14461 tcctcctccc atgctcttag ctgctagtat gtattcgcta ttttttattt gtctaacttc
    14521 tgttctcact atcttagata tattcatcca acttgttatc cctattacta tggacataga
    14581 tactggattt gaatttccaa gtatagcttg tacaaatatt ataattaata ttgaaggtat
    14641 actaagtatg atttctgtaa acctcatcat tgcatcatct acaagttcag atgctgaacc
    14701 acttatacta ccataaacaa ttcctataac agttgatatt acagtggaaa atagtcctat
    14761 aaataaagat atttttcctc cataccatat catagaatat atatctctac ccattgagtc
    14821 tgttccaaat aaaaaagttt tatttggagc taaattagaa cttgctaaat ccatgtatgt
    14881 tggttcatgt gtcattataa aagatgagaa tacacttcca atgactatga ttgataaaat
    14941 aataattgat atatatggta attctttaat ttttttagat aaggttttct ttgtttctat
    15001 atgttcatct tctgctacta aaatataatt ttctcctaca atttcaaaat cactatattg
    15061 tggcttcatt agtatcacct ctatcatatt tcattctagg gtctatttta ttgtttataa
    15121 tttgagctaa catattccca aatactacta aagcccctgt tattaagcat aaaactaata
    15181 acatattata atcatgatac tttgcacttt caaaacaaag tgttcctaac ccaggataag
    15241 aaaaaacttt ttcaactaca taagttcctc ctagtatatg aggtatcgaa atggccatta
    15301 tactaatata tgatggcatt atatttctca ggcaatgctt aaaaacaata gttctattat
    15361 ttaaaccttt tgctttacat aaaagcacat aatcttctct tatttcctct agtaatttat
    15421 ttcttatcat atatgtgtaa taccatagat ggcttaatat aagtactgta agaggtaaga
    15481 ttagatgtga tatcctactt aatatactgg actcttctcc cattgaataa gcaccactac
    15541 ttggaagtat acttaagttt atactaaata taagtatgag tactaaggca acccaaaatg
    15601 atggaataca gttggttatt gttcctaatt tgcatatgat tctatcaatg agcttatctt
    15661 catgtaatgt acaaaatatt cctaacaata aggctagcac aaaggtaagt atatatcctg
    15721 aaccacctaa tattatagtg tttatataga catcatttat aacagaagta acattttgtt
    15781 tatatttaaa tgaaattcca aattcccctt ttgatgcatt tgaaatccat tttatatatt
    15841 gactgtatat tggttcatcc aaaccaagtt ttttcatagc attttctttt tcttgtgtac
    15901 tcattctttc tactccatcc ccatagtaag agattaatgg gtctcctgga gcaagtcttg
    15961 ccatgtaaaa aacaataaaa gataataaaa acattactaa aataaactgc aacaactttt
    16021 tcagtatatc ctttaacaaa ccccaacccc cctttgttgc aataaaaaaa accacataaa
    16081 agcaaacgta aagtaacgtt aaaaccttcg tggttccatt atcattaaac aaaattaaat
    16141 tttctatatt tatctatgag ttaattttag catcatttat taactgtgta aacaaacttt
    16201 ttaaaaattg ttaaaatttc atcaaattat tttatttata cctaaaataa aatttttata
    16261 ttctattctt cattattttc tataaaatta taaaatttta ggttgactat ataagaaaag
    16321 tatattatat tagtttgtat gcaaaccatt taagaggtga ttttattgaa aagtaattat
    16381 gactgtttta gaatagccat gctcttaaaa gaactttatt ctaaaactat gtacacagta
    16441 gaagaaaatt ttaaagaaaa tggattaaca caccaacaga ttatagttat aaaactgata
    16501 gctcataatc aagaacttac aatatcacaa ctatgtgatg aaatgtcttt agctaaaggt
    16561 acggtttcag gcataatcag cagattagaa cagattggat atatagaaaa attcaagaaa
    16621 tctaatgata aaagaaatac atacgttaag tttacaacaa caggatttga atttgccaca
    16681 aattttaaaa ttaaaatgca agaaagtttt gatgatattt ttaaaaattg tgatgaaaac
    16741 gaattgagtg acttagtaaa aaatcttaga aatatattgg caaaagtaaa ggagagataa
    16801 ttatgaatta taaattaata cttgcaatag tatttataac atcagcttta attttctaca
    16861 ctataggtgt atttggagaa agaaaggcaa aaattttaaa aaagaaacat gtaattatat
    16921 tttggcttgg ttttatattt gatactttag gaacatttac aatgagtaac atagccaaca
    16981 gccatacttt tgaagtaaaa tctgctttat cacaaaacct tcatagtatt acaggtcttc
    17041 ttgcaattgt gcttatgctt tttcatgcaa gttgggctac ttttgtccta tacaaagatg
    17101 atgaggaaaa gaaaaaattc tttcataagt ttagtatagt tgtatggact atatggttag
    17161 ttccatattt tataggtatg tttattggta tggctggata gtaaaataaa aagtgtgtaa
    17221 gaaatactgt aggtaaattt gtataagaca aatttatcta catccttctt tcacactttt
    17281 acatatttat ttataatact ccattatttc tttcatgttt cttaatacct atatagaata
    17341 tttaattgtt tctatagtat tttattattt tctcacactt tttcatatat atattgtttt
    17401 actatttctt catatgtata tatgaaatat tccattattt ctataaatgt tttattatct
    17461 ttctcatact ttttcatata tatattgctt cactattttt tcttatttct tcatatacat
    17521 atgaaatatt tcattatttc tataatgttt tattatcttt ctcatacttt ttcatatata
    17581 ttgcttcact atttttttta cttctttatt aaatacttat gtataacata catttaataa
    17641 acgctaacta ttaaacacct aatacttgag ttactcctgg aacttcactc accaaaacat
    17701 tttcaataat agctttgatt gtcatagtca ctcctggaca cccactacat gccccttgta
    17761 gtctaactaa aaccacacca ttttcattta catctataag ctctacatct ccaccatctc
    17821 tttgtaatac tggttttatc ttttcctcaa gcactttttc aactttttct ctcatagcta
    17881 ttccccccag aaatatttta ttagcaaaat tataccaatg gtttaaattt ttgtccatct
    17941 aatgatttat caaattcaat ttttgcttca tcaataattt tagtatccat aaatacatca
    18001 taagcactac aagccagtac tttagctgaa tttatcatag cagaaaatcc tatatttgaa
    18061 cctgctgatg aacatgactg ccatgtatga cttgcaattc caacaggcca agctgccatt
    18121 gcaaattgag ccactggtat tacataactt acatctccaa catcagtaga accagctagt
    18181 cctttatctt tgtcaccgtc aactatttcc ttgtgtatat agctttctac aatagattta
    18241 tcattttgta atgatgttgc tacacctaat ctttcttctc tagtaagagt atcacacaac
    18301 ttttttgcaa attcttcttc ttcaggagta tttttagggc aaccaacaaa aaccatattg
    18361 tcataaacta aatcagtcaa acattggttt ggtatatagt catatattcc atctgtaacc
    18421 ttatactcca tttcagtttc tgtcatcatt gctgctcctt gagctacttt tattaatcta
    18481 tcaagaacgt gttctgcatc ctttgctttt ttacctctaa taaagtacca actttcagca
    18541 aatccaggaa ctacattagg tcttccacca ccattagtta taacatagtg cattcttata
    18601 gagtcaataa catgttctct caaataatta gctccaacat tcataatttc aacagcatca
    18661 agtgcgcttc taccattatg aggagcttga gctgcatggg ctgttatccc tttaaattta
    18721 aattttacag ataagtttgc cagacttcca cctctccaag gtgcatttat atcaaaagga
    18781 tgccatgtaa atgcacaatc tacatcatca aaacagccat ttatacacat aactgtcttt
    18841 cctccacctt cttcttcagc aggacaacca aaatatttta ttgttccatt aaatttattt
    18901 tcttctatta atttttttat agaaacaact gctcctacac ctgctacacc caacaggttg
    18961 tgcccacatc catgtccagc ttttccttct accaatggct ttctttctgc acttacacat
    19021 tgtgatagct ctggtaaagc atcatattct ccaagtatgc caatcacagg tttaccctct
    19081 ccaaaacttg ctataaatcc agtaggtaaa ttttctactt cttcaatttt aaatcctttt
    19141 gactttaggt attctttttg aatgctactt gcatactttt cttcaaaggc taattctgga
    19201 ttttcccata ttttcttaga taaatcaatt aattcttctc ttatttcttc aacattattt
    19261 atgatatact ctttcataat tttcctccaa atatatgtat ttttgatatg ctttacagat
    19321 tatattatat actaacattc tatttattca caattttttg ttaattaatt ttattcatat
    19381 gctcttctat ggagtatatc ttatcttcac tttgcctctt cataaattta atgtactcta
    19441 tcttccataa agtagtttca gttttctcat ttaacataaa ttctaatata ttaatttctc
    19501 tcattctaat atcttcataa aatactacac tacccaatat tgccaattct aattcactta
    19561 atgatgcaaa caacattaaa tactcttctc caaataaaca ttcagattca agaatatcga
    19621 taaagtcttt tttagcattg atattttcct ttattattct tattttttca attatagatg
    19681 tactatctaa tatctcattg aatatctctc taaacgattc atcatccaat ttactttcat
    19741 catcaacaat ataatcttct aagtgttcct ttttattttt actcacagtt accatacttt
    19801 taaaagtgtt actttttatt gagcttagca cagattcaat aaatatcttt tcatattttt
    19861 taatatatct tattacatct tcatcttcta tgcttaatct ttttattaat acttcaattg
    19921 cttccataag tagtaaagtt atttttttat ttattctaat ttctctgtta atttcttcaa
    19981 aatcattata atccaaatta ctatctactt cagtgttaaa actatcatct acttcaatca
    20041 attcaattat attactttct tcagagtcta taactttatc ttcagtaata ttattgtaca
    20101 tatttttaaa ttgtttttct aaaaattcat actcatattt tgaaatttct aaattagtca
    20161 cttcatttcc taaaatagta gaaaaaatac aattattcat aataagttca aataaattta
    20221 caagtataac atcacaatct attttataag tcatcccata gtcataaaat aatttttctc
    20281 tatctttttt cttaaatagg ttacaaaatc tattttctat atctatactc tcaatataat
    20341 tcttcacata ataaataccc ttaacatcca tatcatctag tgccaatgga taatctatac
    20401 ttactggaac gtcctgagct tcaaatttta caatataact tttaaagaaa cttgataact
    20461 ccatcaaggt atcattatat gctattgaat tagtatccaa ttttctactc atcattagtt
    20521 tataaaaatc ttttagccta actaactctt cctctaatat ttcaatgcct ccaaggtaca
    20581 cttttttaac attcttagca gtcaactcca caactctttt ttctatatct ccaagcttat
    20641 ttatatatgc atcaatagca taccatattg aaatcaacag tttttcggca acttctgttt
    20701 ttactgatgt gctttgacca tttgtatatt ttttaatcaa gtccataagt attagagata
    20761 cttcttgttg tatattatat acttctcttt catctatcac tttacaattt agacattcct
    20821 taagtagaga tatggaatat tggttcttta ttaaattatt ttcgtttata ttaatattac
    20881 ttaaattatt taacatacaa aatctctcct atcatatatt aaacttctcc aaatttaaat
    20941 ttctttgcaa attcgtctgc ttctcctctt acattgtcca tattgcctga aaatttttca
    21001 taaaattcac ccagtatttc tataatctca tcatcacata tcttgtcatc agtttcattt
    21061 tttaaataat aaaaaatttc ttgcatatca ttaatagcct ctaaataatt gtccacattt
    21121 gtatattgtg atgtatatat attttctatt aactgttttg taacttttat atctaattct
    21181 attcttccat acccctttaa agtattatct ctcgaattta ttatatcttt tacatcagat
    21241 tcagaaagag ctagaccata atctccagat tctatattag tttcaagcag ttcatttctt
    21301 tctatttcct ttaaattctg atttatagaa ctcaaaataa taggtgatat catttttaaa
    21361 ttctcctttt ttattttttc cattgacttt ttttgttttt tatgctatac ttaaattaat
    21421 gtatattatt ttaccaacca cttataatag tagtgttaaa agtggttttt gtcaactcaa
    21481 aattactatc tcaattaaac ttttcaattt tctcaacaaa aaaataccat catttagata
    21541 tataattttc tataacaact tttaattcag tagttattta ctaaaatcta aacaatgata
    21601 tatttattaa ttttatttat cttttttggc tctacttcta cttttcttta catttgagca
    21661 atttctacaa gaaggtgaac agtattgaga tttagaattt gtaggaatat aaattttacc
    21721 acagtacttg catttattta aagaagaagg ttcacttgta attgcaaagg catagatagt
    21781 ctcaatagct gttttcaaag aatcaaactc ccatgcaata attggtttat ctaattggct
    21841 tatagaaaat cctatctttt gaggattaaa ccctccagac ataatagtaa cactttcagt
    21901 taatagatta gatgtatttt tatatacaca aatttgatta aaatgcttac acatatcttt
    21961 agcaaaactt acaatccaat ctagttgttc agtgtagaac tttgaaaata ctaaatctaa
    22021 aatcaatggt ctctttccaa aaaactttgg agattcttct ctctttgata catctatacc
    22081 ccttctatac tttttaaaat acacatcttc atcacttgca aatggtataa acatgtttac
    22141 atattcatct gtactcaata tattctcttt tgtaatataa ttcttttcaa tcatcagtac
    22201 tctttcttct ccaactatat ctctattata tacacttgaa tatattaaac ccaataatcc
    22261 atactttttt acaaattcaa gtacttcttt ctttagtttc atttcttttg tctcttttga
    22321 tatatcttgt aatgcctctt gtcctatgcg aattatatct actattatat catctgctac
    22381 atcaaatggg ttatacatgg taaacacagc atcttctgtt ggaactatat ataattcatc
    22441 ttcttcagta attttatatt cataatcact atacctaatc caatcacttg caaaattccc
    22501 tattaattct atttttttat tcataataat cattcacacc tttcaatgta atatataact
    22561 tcatattact attacacttt tagtttattc catattttct acactgtcaa ctattctttt
    22621 tatattttta gcatattaaa atttataata gcctttataa tggattttta cagctaaatt
    22681 tcttgtttat tgatgactaa ttcataatta aatttataaa aactatttac atataaaatt
    22741 aatctcttta taattgatat gtaatatatt tttacactta catattacat atcaattata
    22801 aagagaggta tctaaaatca tggatagaaa acgtgaatta aaacaattat acaaagaaat
    22861 gaaatttgac acaggtgtat tcataataaa aaatgatatt actaaaaaga tttaccttgg
    22921 aaaaagtaat gacataaaaa gcaaatttaa ctctctcaaa tttcagcttg gtgctggctc
    22981 atgtatgata aaagaattaa ataatgaatg gaaaaaatat ggtgaaaaag catttacatt
    23041 tgaagtttta gaacttataa aacatgatga taacaaaaca gagaaagact atttagaaga
    23101 actagatatt ttagagatgg tttggttaga aaaattaaag gaagataata cttatgaaat
    23161 ttattccata taaaaaataa tataatataa gaaaagtaga tagtgtctca aaatagacat
    23221 ttagttcata agatactttt ttatataaaa taaagtatct tttcataatt ttaacaatga
    23281 aaaagaggct tatctccatt tgatagcctc ttcccatata ttttatacca ttataaaatg
    23341 aacaatctac taattttcaa tgtatatatt cattttacta ataacactcc attacttctt
    23401 tacagcatcc aaacattgct ctataatact cttatcactt atacctattg ctgttacttt
    23461 actagctcca cttaatatac tcttatcact tttatttgaa actaaaacaa caggagcatt
    23521 ttttgcaagt ggtgatgcaa ctaaagcata aactaaatca tctccacttg ttatatagaa
    23581 ctcttttgct ccattataga acttttcaat aacattttta ttagtttgat atctatcaac
    23641 accaccaatt ctagttgctc tatatctgct cactatgtaa tcacttaatg tagactctcc
    23701 tccaatagca tatactggaa caggagaaac tccaaaccat gggtctaagt ctgtgtcttc
    23761 ttctgctgaa ggtatatctt ttgtcaatat tataggagct ccatctctat aagcaacaga
    23821 tgcaacactt actgcatcag cttcatcctt aaatccattt actaaaaaca tcttatcact
    23881 gtttttaatt ttttctattt cacctgcaat tttataactt gtatctactc tatctacacc
    23941 atcaattctt ataacttgca ttcttctcat aagtaacatt ttttctacat ctttacttat
    24001 agaattttct ccacctatta catatatctt agtaactcta ttcagtcttt gaagtgttgc
    24061 ttctggtata tcatccttat tagttaataa aatagctgca ttatttactc ctgcaagacc
    24121 acttgcagca atcccatcta ccattgtaga gtctgcattg ataagaactg ctgttgtaaa
    24181 atcttgctta tctgaaataa gcccagctgt ttcatattta tctactcctt gaatattttc
    24241 aatcttatct agtgcgttta ttgatgttgg aaaactaaca cttaacagta atgctgaaat
    24301 acctaacgcc aatacctttt tactaatcct cataaattac ctccctaaaa caaatatatt
    24361 tagtattagt ataatataaa atcatttaaa aatagtgtaa aaatagttac acttttttat
    24421 aatttaaata attttagact aaacacaatt catccattaa aataacttca tttattctat
    24481 atgctaagcc atcccacgtt ttttttgaca tatctacaga tgttttctta tttgagttat
    24541 tcaataaaga ttgtatctta gaatctattg cattatacaa attattttca aaatcatata
    24601 actcttcatc ttttggaata ccttctttct ccataaaagg tagagaaaca tattctatct
    24661 ttccagaatt attaatctca ctaccaatcc actctttaac accaagaata tcagtagtga
    24721 ttacatctgt gccacaagat aaagcttcta aaactacaac tggtaatccc tcataaaatg
    24781 atggtaacac aaatatttgt gacctgttga atagctcagc taaatctctc tgttctaact
    24841 ttcctaggaa attcatttta aatggagact tacttgccag tttaactatc tcattatatg
    24901 attcgatatc gcttccagta cctgcaaaat tgacttctat taaatcttta ctatatttta
    24961 atttagatat agcttttata aatggaatca gtccctttga tttacatatc ttacctgcaa
    25021 atactatttt tattttatca tcctttattt tataattttt attataaaat atatcatcat
    25081 tataaccact accaattaca actactttac tttcacttac tccaaaagtc tttataatat
    25141 catatttctg attttcatga agagcaaata ttaaatctaa tttccttata tttgctatta
    25201 tgtattcttt ctctaaatca atggtattta actgtcttaa acaagttcca tgacaaatag
    25261 acataacctt tatatctttt accatttccc taacaaatgc agttaagagg tacaaatgat
    25321 gacatattat aagttctggt ttaaaatcat tcatagcctt atctatattt actttaaatt
    25381 gatatttcaa tctatttatc atgtctatat ttaaatcttt atatcttgta ctttcatatg
    25441 gcatagaatc actcatacct acaacaggaa aatttaactc cccacaatta tactttactg
    25501 gataaaaact tacttcactt ggaaaacaat ttacatcatc atttacatct atacctgcaa
    25561 ttacagcttg cttatgaccg attttttcga acccttttat cataccagac aaatatatac
    25621 cactacctgt actatttggt ttttgtgtta ttatatgaag aattttcact tttcacttgc
    25681 ctcctttcta aaattaaata gttttataca gtctaatcat tatatatatc taaatataaa
    25741 caagatgaat catcttttac tttaaatctt ggcactttaa ccgctttata gtcattatct
    25801 tcaaaatccc ttactttatt atgtatatat cctataccaa atttttctgc tatacgtatc
    25861 aattcttctt cttttattag attatatcta tcacatgcac ttgtaaaacc atcactcgtt
    25921 agcatcaatt gaaaatcttt ttttatttca atgtatccat atatagaatt ttccacagct
    25981 tcctcatcaa aatccaatat ccaatagccc tcttttgtat ttttcttcaa tctattgctt
    26041 actattgtat ccataacact agttctaatc tcatcaaaag ccatctcttc caagttagaa
    26101 agtttactca tttcatcaaa tacagtatta tcaaatttac aaatactcct atcttttata
    26161 actttggaat ctccattttt aataaacaaa gcacaatctc ctaataaaaa atactctact
    26221 ttattttcat gaaattttac aacaactata gaactagatg gtaaatctaa tttttcaacc
    26281 ttaaacccat ttaatcttag ttcatactct ttttttactt ctattattcc agtattaata
    26341 atactcttta atgattcttc tttatttata tttttatata aatacctatt ccaccactga
    26401 acataccacc ttgcgtcact ttcagttgac actaaatttt ttccattaag tcctgtagca
    26461 ccatctaaaa cccatgcccc aaatggattt ataccgacta catcttcatt aaatttagct
    26521 gttccttgat tacataatag gctatagttt cttatattta aagccatatc aacctccaaa
    26581 ttgaatatct aaaaataaag ttctatttaa aattatacag caaataaaaa aatctacaac
    26641 aaaacttgtt gtagattttt acttaatcta tattatcttt atactttcag tattttaatg
    26701 atggaataat ctatttttag taaatgccat gactatgtta tacttattac aagttaatat
    26761 aacattatca tcacgaatag aaccacctgg ttgtgctata aaacttaccc cacttctttt
    26821 tgcacgttct atattatctc caaatgggaa aaatgcatct gaaccaagtg ctactcctgt
    26881 taaagtttta agccatgctc tttttccttc tccagttaga ggctctggtt tttcttcaaa
    26941 aaagttttgc catatgccat cagcaagcac atccatataa tcatcagaaa gatatacatc
    27001 aatagtgtta tctctatctg gacgacctat atccttttta aattttaagt ttaatacttt
    27061 aggatgttga cgaagatacc aagtatcagc tttgttacct gcaagtctag tacaatgaat
    27121 acgtgattgt tgacctgcac ctacccctat tacttgtcca tctttagcat aacaaacaga
    27181 atttgattgt gtatatttta atgtaataag agctataatt aaatcacgtt ttgcactatc
    27241 tgtaaaaatt ttattatcag ttggaatatc ttttaatagg tcttcattta ttaatatttc
    27301 atttctacct tgttcaaaag taattccata aacatccttt gtttcaatag gttcaggagt
    27361 ataattttca tctattttta caatattata gttaccttta cgctttcctt ttaatatttc
    27421 aagggcttca tctgtatatc ctggagctat tataccatca gaaacctctc ttgctataat
    27481 agttgcagtc tccttatcac atacatctga aagtgctata aaatcgccat aagaagacat
    27541 tctatctgct cctcttgcca ttgcataagc acaagctaat ggagaaagct ctaaatcatc
    27601 aacaaaatat atttgcttta aagtatctga taaaggtacc cctactgctg ctcctgctgg
    27661 acttacatgt ttaaatgaag ttgctgctgg aagacctgta gcttctttta attccttcac
    27721 aagttgccaa ctattaaatg catctaaaaa atttatgtat ccaggttttc catttaatac
    27781 ctctatagga agttctccat tcttcatata aatctttgat ggtttttgat ttggattaca
    27841 gccatacttt aattctagtt ctcttgccat atactcatcc ctctctataa taaattaatc
    27901 ataagtttga ttaatcctat attaattaaa ttattcgtac taataaaatt atatggcata
    27961 tttatccatt aatcaactat attttttaaa actcctattc cttcgacttt acattcaact
    28021 atatcgccat gctttaaata tacttgagga ttcatcccca tagcaacccc tgctggagtt
    28081 ccagttgaaa ttatatctcc tggctttaga gtcattcctc ttgataaatc atttattata
    28141 gtaggtatat caaatataaa gtactctgta ttagaatctt gtcttacttc tccatttact
    28201 acactactta tatctaattt taaaggatgt tcaaattctt ctttatgtac tatacaaggt
    28261 cccatagatg tatgtgtgtc taagcttttt cctctaaacc attgaacatg tttatcttga
    28321 agtgctcttt cacttatatc atttactatt gtatatccaa atatatattc ataagcatct
    28381 tcttttgata tatttatacc ttctttacct atgattactg caagttctga ttcatagtcc
    28441 aacttctcta ccaaatctcc atgtaagctt atcttatcat ccactccaat tattttgtct
    28501 gctctcttag aaaaataaat tggataatct ggcataacta cgttttttat aactccatct
    28561 gggatttcat tgacatggtc tttatagttt agacctaaac atataatatc ttttttaggt
    28621 cttacaattg gggaacataa tttaacttct tctaacttat aacactttgc gttttcaaat
    28681 gaattgtttt ttattttttc taaatctcct gtagatacgt tttcaataaa atcattcata
    28741 tcagtatata atttacttaa tcctaaacta ttaatttcat atatactatt tgtttctgaa
    28801 ttaaatacac ctattttttc ttcattgcta ctacaaaaag tcacaaattt catttttatt
    28861 cctccattca ataagttgat taatattatt taatttttat tagtttatat aaattgtaat
    28921 tcataatatt tatatgtcaa tac
//
LOCUS       DAYAOX010000023        28893 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.023, whole genome shotgun sequence.
ACCESSION   DAYAOX010000023 DAYAOX010000000
VERSION     DAYAOX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.023"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            103..978
                     /locus_tag="ACGI57_000866"
     CDS             103..978
                     /locus_tag="ACGI57_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HFG9715952.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDINTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            1337..2641
                     /locus_tag="ACGI57_000867"
     CDS             1337..2641
                     /locus_tag="ACGI57_000867"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/bile acid-CoA:amino acid
                     N-acyltransferase family protein"
                     /protein_id="HFG9715953.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNNYY"
     gene            2825..4216
                     /locus_tag="ACGI57_000868"
     CDS             2825..4216
                     /locus_tag="ACGI57_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFG9715954.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            4393..4980
                     /locus_tag="ACGI57_000869"
     CDS             4393..4980
                     /locus_tag="ACGI57_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9715955.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKSDWNLSI"
     gene            5013..5804
                     /locus_tag="ACGI57_000870"
     CDS             5013..5804
                     /locus_tag="ACGI57_000870"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9715956.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5985..8402)
                     /locus_tag="ACGI57_000871"
     CDS             complement(5985..8402)
                     /locus_tag="ACGI57_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9715957.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(8415..9080)
                     /locus_tag="ACGI57_000872"
     CDS             complement(8415..9080)
                     /locus_tag="ACGI57_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9715958.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(9162..10193)
                     /locus_tag="ACGI57_000873"
     CDS             complement(9162..10193)
                     /locus_tag="ACGI57_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9715959.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSRICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(10183..10869)
                     /locus_tag="ACGI57_000874"
     CDS             complement(10183..10869)
                     /locus_tag="ACGI57_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9715960.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(11027..12091)
                     /locus_tag="ACGI57_000875"
     CDS             complement(11027..12091)
                     /locus_tag="ACGI57_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HFG9715961.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            12353..13024
                     /locus_tag="ACGI57_000876"
     CDS             12353..13024
                     /locus_tag="ACGI57_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9715962.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            13026..13934
                     /locus_tag="ACGI57_000877"
     CDS             13026..13934
                     /locus_tag="ACGI57_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9715963.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            14011..14697
                     /locus_tag="ACGI57_000878"
     CDS             14011..14697
                     /locus_tag="ACGI57_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9715964.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            14694..17249
                     /locus_tag="ACGI57_000879"
     CDS             14694..17249
                     /locus_tag="ACGI57_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9715965.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(17443..18642)
                     /locus_tag="ACGI57_000880"
     CDS             complement(17443..18642)
                     /locus_tag="ACGI57_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HFG9715966.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            18761..19648
                     /locus_tag="ACGI57_000881"
     CDS             18761..19648
                     /locus_tag="ACGI57_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9715967.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            19838..20395
                     /locus_tag="ACGI57_000882"
     CDS             19838..20395
                     /locus_tag="ACGI57_000882"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9715968.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(20634..21494)
                     /locus_tag="ACGI57_000883"
     CDS             complement(20634..21494)
                     /locus_tag="ACGI57_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9715969.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            22044..23396
                     /locus_tag="ACGI57_000884"
     CDS             22044..23396
                     /locus_tag="ACGI57_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9715970.1"
                     /translation="MIFICLILTVIGIFLSLITIQKPNGEFIRSDWPKKFTENFNEQI
                     VFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPKEHKDYYSNSELLALANQ
                     NNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGKFIGGKSIVFSIIGIIFL
                     IILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVFADIYNSLNILNEEIHKS
                     DEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSSTNYSPEKVYKYASIMLKN
                     IEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSIDILNYPEYESNIVEFKSN
                     IDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHVTLNERIEIIISDNGKGMSRE
                     EASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNISVESIPGLGSSFYITFPI
                     "
     gene            23615..26092
                     /locus_tag="ACGI57_000885"
     CDS             23615..26092
                     /locus_tag="ACGI57_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9715971.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGNMNEEFIEDVRHMEGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGVTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            26107..26793
                     /locus_tag="ACGI57_000886"
     CDS             26107..26793
                     /locus_tag="ACGI57_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9715972.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            27067..27900
                     /locus_tag="ACGI57_000887"
     CDS             27067..27900
                     /locus_tag="ACGI57_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9715973.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSWMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            27938..28399
                     /locus_tag="ACGI57_000888"
     CDS             27938..28399
                     /locus_tag="ACGI57_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9715974.1"
                     /translation="MKFGGVLLIVKDINKSKKFFEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQV"
     gene            28430..28678
                     /locus_tag="ACGI57_000889"
     CDS             28430..28678
                     /locus_tag="ACGI57_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9715975.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVKDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
BASE COUNT        10904 a         3459 c         4242 g        10288 t
ORIGIN      
        1 catcaaatta ataattatag tataaataag tttaatatta tgattataaa gtttatatta
       61 aatttatttt attgatgaaa ctaatgggga ggttaaaact caatgattac ttttgatgta
      121 aatggagcta ttatcagatt tgatgataca aaaaataatt ataataagat aaggaaaatc
      181 tttaaattat atgctttgga aatatctaaa aactttgaga gagattgttt aaatagtttt
      241 caaaatctaa agcaaatatc tgatagaggt ttaagtttag gagaaaaata tatagaagag
      301 tcttttaaga aaggaattga gactatagta agttttggaa ttataactat agatatcaat
      361 acctttaaaa gtgtttattg tgaaaaatat ttagatttta agagactttt taataacttg
      421 aataaaactt ttattatacc aaataaaaat aaaaaaaatg gccacgcgag cttcttagaa
      481 atagaatcga caataaagaa aatgagcaat tatttataca atgattgttt taaaattcat
      541 tatgctgttg tagatgccct tatagaaaat cgtataactg cggtacattc ttatattgat
      601 gaagaatcta taaaaaaatc taatgcctta tttaataatt ataaagatgg ttttattggg
      661 aaggctgacg aatgcaaagt agtaaaacaa ataataactt taaatccata tagggaagat
      721 gtatacgaat ttttggtaaa agaagatgga gattttagta aggaaattga gagattgact
      781 gaatttttag gctatgatat aagagaatat aaagcgtcat taatggatat ttatataaat
      841 gagttaatgg agagcgatga aaatgatgtt gaaaatgcaa aagaaaagat tgaaaaatat
      901 gctagatata ttggatgtag tgatgagaat ttatatgtag caagagttaa tgccatatat
      961 acttttgcaa atgcataatt ttattaaaca ttaaatatta gatgttcgat tatataaatt
     1021 taaataaaaa aatatcctta ttaataacaa atagtttata ttaaaaacca ttcttaaaat
     1081 gagtggtttt ttattttgaa tatatatatt ctaaacctaa tagcattcct atctttaatt
     1141 agccaaaaat caactggatt tcatatagac tacataattt taaattgcta aaaaagacaa
     1201 tatgttgaca aaaatagaat atgttgataa aatgaaatag tcaatatggt gtcttaattt
     1261 aaaaatttat ttatgttgta ttagataaag ctgttttata taaaacataa ataattatta
     1321 taaggagaaa tagattatga aagtaaatgt atacaatgta gaatcatttg tggatgaaag
     1381 acttaagatt atggtttcgg acttaattcc aaatactaaa cttaaaataa gtgtgaaaat
     1441 ggaatttcca tggtgcaagg gagaagaatt ttcatcttat ggagtgtttt gttcaaatga
     1501 aaaaggagaa gtagatttag atttagttga acctatagaa ggtacttata aggctgataa
     1561 ttgtatgggg ttaatttatt cgctggaaaa atctaaaact gaaggtaaaa attttgcaga
     1621 aaatatatct attgataaac caataattat gaatatgata tttgaaactt ctattgaaag
     1681 aaaagaagtt gaattaaaga gaatatttaa atcagaagat attaccacaa aatatatatc
     1741 tgatgagttt ataggaaaat tattttataa acaaaattct aataataaaa caattttaat
     1801 gctaggtggt tctgatggaa atttagacgc tctagacctt ttagcagctc cacttgcatc
     1861 tagaggattc aatgtactaa cagttgcata ctttgcttta gaagggttac cagataaatt
     1921 agaagaggtt ccattagaat attttgaaaa agtatttgag tggattagtg aaaatgaaat
     1981 taccagtact aaagaaatat ttgtacatgg gacatcaaag ggtggagaat tggccttttt
     2041 acttgcttca agatatgaac aaattaaaaa ggttgtagta tcacaacctc acatttattg
     2101 tttccaagct cttaatggat tgatgagtgg aaatgatact tcatcatggt catataaagg
     2161 agagccattt ccatatatca aagtagacaa tgatatattc tttgaagaac agaaaaaaaa
     2221 tatatcaaaa ggtattccat ttggatttaa tagtacttat aagaaaagtc ttaaaagagc
     2281 agagaataga aaagaggcta gaataaaaat agaaaattcc aaagcagata tacttatgat
     2341 tgctggaaaa gaagataaca tatggaatag ttatgaagct tgtttaaagg cattagaaat
     2401 ctttgaagaa aataattatc catataacgt agaattatta acatacaata atatgggtca
     2461 tccgttacca attccatata ttatgcctct aaaggagact ttatgtatga gtatgagtgg
     2521 agggattttt tcttcaggtg gaactgtaga aggaaattca aaaggacagt ttgaatcatg
     2581 gaatagaact attgaatttt ataaaaaacc tttatcaagt gatttaaata actactatta
     2641 agtgatttaa gtaactaaaa atgaagcggt ctttctatat atgcacaaaa tctatcaatt
     2701 ttatagattt aatttattgt ttatataatt tgcatgttat atactaattt taatgacatt
     2761 gataatttat aaacaattat aaagttgtaa acttataaga gttattgaaa ggtggagtga
     2821 agttatggaa agactcgcta gaagggaaca acctcatata aaatttggaa tgtttaagat
     2881 aagaattcca tttatacatt acagatttga gaaaccagaa gctattcaag gtataatatc
     2941 aagtatgaca agtttaggca caattggcct tagtacacaa attttgggat taccctatga
     3001 agtggcatgg agtatggcta tcattaactc aatattatac tgtttgcatg tttttatggg
     3061 agacccagtt gtacctggct ggattacagc ttctataact cttacaactg catatttgtt
     3121 aaagttcagc atgggcattg aaagaataca agcattaaca gctttacaaa tagatttagg
     3181 aataatattt attttaatgg gtataactgg tgtagctgga aagttagtta gtaagatacc
     3241 aaattctatt aaaggtggaa tactaatggg tgtcggtata tcgactataa tatcagagtt
     3301 taatcctaaa gggagatttg attcttatcc tatatctata acagtaggaa ttttggttgc
     3361 ttgttttgta atgttttctg aaagatttga tactctaaaa gttaagaata aatttttatt
     3421 tcatttagga gaatatggtg tagtatcagc tattttagtg agtttaatag taggtatatt
     3481 tagtaaagaa atagaaattc ctagatttag ttttgacaat ttagtttaca tagtagattt
     3541 taaaaatctg ataaatacag tatctccttt tggaataggt tttccaagtg taatgttatt
     3601 tatacaaggg ataccaatgg cttttatgat atacataatt gcctttggtg attttataac
     3661 aggtgaaaac ttagtacttt ctgaaagtga aaatagaaaa gatgaatata tagatttcaa
     3721 ttcaaatagg tctaatgtta taagtgggat aagaaatatt tttatggcaa tcttttctcc
     3781 atatatacca atgtgcggtc cattagctgc gacacttaca ggaagtgttg ctcaaaggta
     3841 caaggttgga aaggaagcca tgcagtctgt ttttagtggc atgggtacat tagtatgggt
     3901 ttcagcaatt tttatctgtt tttatccaat tgcgcagatt gctacacctc ttataccatt
     3961 agcattgtca gtaacacttt tagttcaagg atatttatgt acaaagctat caatggagct
     4021 atgtaataca ggtgttgaga agggattgat tggacttatg ggaggtgtaa ttgctgctaa
     4081 aggcggaaca tggggacttg ctgtaggttt tatactgtat ttcattttaa ttgattctaa
     4141 aaaaagaaaa gaaaaagata ttaattcaat agaagattgt gtatatgaaa tattagacaa
     4201 aaagaagagt atttaaagga aaatctgagt tgctgttatc tattaataga ttaattgcaa
     4261 ctcatgtttt atttataagt tttaggaatt gcaaaaaaat gcaaagcttt ttcttgacat
     4321 aatttcttga tatgttacta tagttatata acacatgtgt tttataaaaa tttaatggag
     4381 aggtgaacat atatgccacc taaagtaaag ataacaaaag aaatgatttt gaatacagtc
     4441 ttggatatta caagagaagt gggatttgaa gctgtaaatg cgaggagtat tgcaagtaaa
     4501 ttacagtgtt ccactcgacc aatttttact tgttatgaaa acatgaatga attaaaaaat
     4561 gaatttcttg cttttgcata tgaatattat aatcagtatg ttgttaatta tcgtaattct
     4621 aaaagtgtaa gcccttattt actgcttccg ctttcataca ttgaatttgc tcaagaagaa
     4681 acgtatttat ttaagttgtt atttataaat gatttagatt tgatgatgaa agaggcgaat
     4741 gatttttata aagaaattga caatgaaaaa aaggcaagac ttttttcgga aagcattgga
     4801 atagatttgg aatgtgcaaa agtaatattt ttagatttat ttctttatac tcatggcata
     4861 gctgtcttaa cagcaacaaa aaaattaaca ttagatagat atagtgctga aaaaatgttg
     4921 aaaaatatat taacagcttt tataagacaa gaaaaatcag attggaattt gtctatttga
     4981 tgtttattaa ctaatataga agaggtgaaa ttatggaaac aaaacaatac ttaattgact
     5041 tttataatac ttatgatgaa gatagtcgac ttgcattaaa acatggaatg gtggaatttc
     5101 tcactactat gcattacatt gataagtaca taaaatcagg tgattgtgtg cttgaaattg
     5161 gcgctgcaac tggtcgttat tctcacacac tggcacgtca gggatatgat gttgacgcag
     5221 tggaattagt cgaacataat atagaggttt tccataaaaa tactcaatca aatgaaaata
     5281 taagcattac tcagggtaat gctatggatt tatctgtttt cccagataat aaatatgata
     5341 ttacgttact gttaggacca ctatatcatc tttataacaa agaagataaa caacaagctt
     5401 tacatgaggc aattcgtgta acaaaacctg gtggtgtggt ttttgctgca tatgttatat
     5461 ctgatggctg tcttattgac gaggggttcc atagaggaaa tattaatgta tctgaatata
     5521 ttgaaaaagg tttgattgat ccacagacat ttgctgctaa gtctgaacca aaggatttgt
     5581 ttgagcttgt acgtaaagaa aacattgatg atttgatgtc tgcatttaat gtaactagac
     5641 tgcattatgt cgcatcagat ggtttggctt tgtatatgcg tgaagcagta gactctatgg
     5701 atgatgatgc atttgcatta tatttaaaat atcaccttgc tacttgtgaa cgtgaagatt
     5761 tagtaggggt tacaagtcat gcaattgaca tatttagaaa ataaggaata tgcttgttta
     5821 tgtctctggt gatgttgctc ggagatataa gtaagtgtat ctgtataaaa taaaaacaag
     5881 tatagggata gaaaattatg taaagtcaaa attttctatc cttttagctt ttaaaaaatt
     5941 atgttaaaac ttgtataaaa aatggctatt ttttatatac attcttactg agaacgtaag
     6001 cgttctgtaa gtgatttttt ttctatattt cttacaagta attcagtcac taaaacttgt
     6061 ataccaatta atacgaatga aaaaattaaa atagtaaata tagaaaattt aaaattaata
     6121 aagtctaatc taattacatc ttttataaaa gtgcaaagta gatttgaacc aaaataacct
     6181 agtatacttg aagtacatat agctttgata cttatgttta gaccttctct ataaagcata
     6241 tttcgcagtt gctttcttgt cattccaacg gcttgaagta atgcaaactc tttttttcta
     6301 attaaaacgc tgtttattag ggtattggtc atatttagta taccaaagca agaaattaaa
     6361 ccaacaaaaa tgtatgaagc tataattata tattgaaatg ctctagtaat cattctataa
     6421 tcatcttcat atgaataaag cattaaattt gaatttttct gaattatact taacaacttt
     6481 tcttcaactt ctttagaatg ctttttatct attaaaatag aaaaatctaa atttgtatta
     6541 tatggtgtta gagattctaa agttttgtct gaagcaaaaa acatacctcc tgtattggaa
     6601 ttgtcaatga ttcctataat tttcattttc tttttaataa tatcatttcc actaaagata
     6661 ttaaatgtaa ctgtatctcc aacttttaaa tttaatccat aatattttgt tctatattta
     6721 tttataatta aactatctga atctaaatgt gtatagtcta tttttccact gacaatatat
     6781 tcttcaagtg attttgaaag ttcaggcgta atacctttaa ttagagtaat aaattgtttt
     6841 tcgtctctat ctttgatgta aggttctata atattacttt taattgattt ggattcaatt
     6901 aattttttta ctccatgaat agatttaatt tcattcttaa gagaatctgt tagtggatta
     6961 ttttgttgga tttctgtaat atgctcatat aagttatcac tcttaatgct aattatataa
     7021 tcttcgttat agacaaattg ttgagatagt tgttttttga aatctattga attagcaaga
     7081 ttagcgattg aaataaataa aactccacta atgcttagcg atattaatgc aatcaaattt
     7141 ttctttctat ttgactcgat attctttaat gctagatttt ttatatttat ataactaaaa
     7201 ttttttgctt tttttctaaa tttagtttct gtatattgca tggcttcaat agcagatatt
     7261 ttagaaataa ttctagctgg tttaaataaa gaaattctaa cagttaaaaa cattaccagt
     7321 gataaaatta aaattataaa taaatcagct atcatttgcc atgctaatgg ttgaattatg
     7381 taagcaatta tacaagaagc tataacccct aatggaataa aaataattga caaaatattt
     7441 ccttgtagag aaataatttt ttttaactga aaagaagtca tacctaacga tttcaactta
     7501 gcatacatct gaactgaatt aacaactgaa atataaaaaa tactatatat aacaaaaacg
     7561 ctagaaaaaa gaacaactat tccaactact aaaactgtaa taatctgctc accatcaatt
     7621 aaatttgatt caatatagtt atcatttaag tatatattat atttcttaat acctaaagat
     7681 aaaccaattt tatttaactg tgtcttaatt gaagctgaat ctttctctgt attttcttct
     7741 gatagtttaa ctaatacatc tacagtatca ggtcttgact cgtcaaaagt attactttta
     7801 ttttgtaaag gtgtagaagc tatattctta aaataatcat ttgaaacgac tgtatagtat
     7861 tgcttggcat tatcttgata ataattttct agaaatccaa caataataaa atcacattga
     7921 acttctttat tcgtaatatc atcataataa tttaatttaa tgctatctcc aatatcttta
     7981 tctaaaccaa attcctgtaa atatttttct gaaatagcaa tttcattttt atttgtaggt
     8041 agtttgcctt tcaatggttg aaaattcata agtttaattg ctgtatcatc tgcataaaca
     8101 atcgacataa tacaatctgt ctttttatta cttccaatta atttgtaagt tcctacgcca
     8161 ctaaagtttt tatctttata tagtatattt tttgcttttt catctacaga tttatatata
     8221 gcatgatatg gagatgcatt aactaaatct acagaggcat tatatgaaac taaggtcact
     8281 gttaataata aacatgtaga aatcattata atgatacctg aaagatatgt tttaagttta
     8341 ttaattttta aatcatttaa agctattttt cttataaatg tgcgattatt atttctaaac
     8401 atatactaca cctcctagtt aacaattttt ccatcttcaa tacgaataat tctatcagtt
     8461 agttgtgcaa tttcattatt gtgtgtaatc attacaattg tttggttgaa ttttttactt
     8521 gtcactttaa gtaaactcag tacatctgaa ctagttctac tatcaaggtt acctgttggt
     8581 tcatcagcta gtataatagc tggttttgtt acaagtgctc tagctatagc aacacgttgc
     8641 tgttgaccac cagaaagatt acttggtata tcatataatt tttcttcaag agaaagcata
     8701 tttataattt catttataaa atccttatct atggtctctc catccaatcc tattggtaag
     8761 ataatatttt catatacatt aagtaaagga acaagattat aattttgaaa tataaaacct
     8821 atatttctgc gtctaaagat tgtaagctgt tcatcagaca tagtagataa ttccttaccc
     8881 tttataataa cctttccaga tgtaggatta tctaatcctc ccatcatatt taataatgtg
     8941 gatttgccac taccagatgt acctacaata gaaataaatt cgcctttttc aatagataga
     9001 gttacaccat caagagcttt tgtaatgttt gaactttgtc cataatattt ctttaaatta
     9061 attacttgta aaatactcat aataatacct ccttaacatc ttgtaaatac agtataaaac
     9121 caaaatgttg cagaatcatt acaaatgaaa tatttaaaaa tttatttatt aggtaagaaa
     9181 atagaaaaag tcgacccttt tccaatatct gatgtaactt taatatagcc accttgaaga
     9241 gatataattt ttcttgaaag atagagacct attccaatac cttctatgtc atggacatct
     9301 tcttcacgat aaaatctttt aaagatttct gcttggcgac tttctgatat tccttttcca
     9361 ttatcaataa tatctatttt tgtatacata acccaacatt ctgtagctac acgtatctta
     9421 ccattagaac ttgtgtattt tacagcatta tctaatatat taaaaatagc ttcagaagtc
     9481 catttcttgt catgaattat atataaattt gggtcacaat caactgtaac ttcaatattc
     9541 tttttttcag catcaaacaa aattccacta agtgctgtag caattgtttc ataaattgaa
     9601 tttttctttt tagatagtgt gattatacct gtctctagtc tggaggtttt taccatagat
     9661 tgcattaaaa aatctaattt atctaattgg ttttccatat ccattaaaaa atccttttga
     9721 atttctgttt taaatgtttg atttagtaga gtagtattaa tcatttttaa atttgcaata
     9781 ggagttttaa tttgatgaga tatatcagaa actagttctt gtaaatcagc tttctctata
     9841 gcaatactat tgcgattttc ttgaataact tcatacattc taattaatct gtgattgata
     9901 cgtgataaaa tagtttcttc ttctaattca atttcaatat tatcttcact attcatcata
     9961 ctatctaaag tgtgacatat tctagaacaa aatataacta tttttttatg aagtaaaaat
    10021 attattaatg ctataaatcc acaaagtact attgtatata ttaataatag agcaattagt
    10081 tttaaatcat cagttattag ataaattgag atactaaata aaatagctgt aaaaattata
    10141 ccaacagcaa ttattaaata aatagaacta aaagaaaatt ttttaaaatt catttctttc
    10201 acctaaccac atatatccca taccatatac cgttttgata tacttaagat tgtctttttc
    10261 aattttgctt ctaatcctac ttataacaga tgttaaagta tgttcatcta caaagttagc
    10321 atctatatcc cagagttttt ctaaaagtac tttccttgtt aaaatacttt taggattttc
    10381 agtaagcagt tttaaagtac gatattctaa tggtgtaaat ataatttgat ttccatttat
    10441 attggcagat aactcagaaa agttaatttc aagatatcca tccctatagt aatcttgttt
    10501 tgtttttatt gtaagatgat ttaaaaaagc tttaattttt tgtttaaata tattgatatt
    10561 aaatggtttt gttatataat ccaaagctcc aagttcatag cctttaatca tatcacattc
    10621 catatcatta gcagttaaaa aaattattgg tatattgtaa ttttccttta tttcattaca
    10681 taattcaaac ccacttttat ctggtaaatt aatatccaaa attattaaat caaattcaca
    10741 ttcaaaaata gactttagtg cagatttagc tgtaaatttt gaagtaatta tatatccatc
    10801 ttcaattagg ttatatgata gagttttatt taatatagaa tcatcttcta ctactaatat
    10861 tttttccata cagttactcc ttttagttta atattttata atgtcaatta tagactaaaa
    10921 acattgctga attgttataa acgagaaaaa gtatttaaaa atttatatat ttaataaaga
    10981 acctgtccta tgataaattc tagtacagat tctttattaa ataaaattat ttcaaccaag
    11041 atggatttcc aggtataaat attcttttac caaattttga agtatactca tctaaagctt
    11101 tttgaataag ctcattatta cccttaaatg aagtagtatt ctttatagtt tcgagatttt
    11161 ggctaaatgg gcaagcagat atacacatac cacaatctgt acctaaatat ctccatttaa
    11221 tatagcatgt ctcatgttca attacccaat tatattttcc atcatcacca attttaatat
    11281 cattagaaag cgaatgagaa gggcagttaa aagagcattt tttacagacc ttacaaaaat
    11341 cttctagtcc aaaatcaaca tattcatctt ctaaaagagg aatatctgta gtaacaacac
    11401 ctaatctaag tctagagcca taatctttat ttgtaagtat agcatttctt cctatatctc
    11461 ctaaaccagc atcacgagct ataaaaacag gcattactaa ataattagca tccatatgat
    11521 ttctagcatc atatcctaaa tttcttatat agtaacttaa aatcatgccg acgatagatg
    11581 catccacata gcactttgat gtttctataa cttcacatat cattggagct ctatttatca
    11641 tttctttatc catctcacat ccaaatacaa ttgcatactt atgagttaga ttaatttctt
    11701 caccgtaatt ttcttcatgt ctacccctat ttgtataaat atgatagtct tttaatttag
    11761 ttataccaac tacagaagct ccatactgct ttgcaagacc cttaattttt tttgtcattg
    11821 actttgcatc tacatctact ttagtatcag aaacttttcc ttcgcataaa gatttgatat
    11881 cagataaaaa atcgaatgca ctacttgcca ttggagagtt taactcattg taagtcattg
    11941 ttccttcact acataaattt ggtctatttc ttatactatc atcaatttct tttttgtcag
    12001 gatttttctt ataatagtca ttataagcag tactatcttt tttgtaattt gctcttgcaa
    12061 acatagtatt tctctcatca acccttttca attctattcc ccctagtaaa aatatattta
    12121 aaaaatataa ctttattgtt gtataattta attatataag aataaagtta tattttcaat
    12181 ttaagcgtaa ttaaattggg aaatatataa ctatatgctt gattataatg tttttatttt
    12241 gaaaacaaat ttaaatacaa tctttttagt gaaataaata aaattaaaat atttttagta
    12301 tgataatgca agaaaataat tatttatcta aaaaatctaa aggagagtaa aattgaactt
    12361 attaattatt gaagatgata taaacttaaa tgaaggtctt ttttatgcat ttgagaatga
    12421 tggatttaat gtatttaagg cttatacaaa acaagaagga ttaaatatat ttaatagtaa
    12481 aaatatagat tttataatct tagactgcaa tcttccagat ggagatggat ttgatgtatg
    12541 tcaaataata agggaaaagt cagatatacc aattataatg cttactgcaa gagactcaga
    12601 aattgatgaa gtaaaaggat tagaaatagg gcttgatgat tacattacaa aaccattttc
    12661 tttatctgta ttaaaggcaa gagtcaaagt agcaataaga aaaaaatcga ataataaagt
    12721 aatatattcc aatggaataa aattggacca aaagttacta aaagtatata aaaataaaga
    12781 atgtttggaa cttagctctg tagaatataa gcttctaagt tatctaattg aaaataaagg
    12841 tcaaatatta ttaaaggaac aaattttaca ccatatttgg gacagcgaag aaaattatgt
    12901 tgatgataat attgtatctg taaatataag aagacttaga gtaaaagtag aggatgaccc
    12961 atctaatcca aaatatataa aaacagctta tggaatgggt tatttatgga atgaggtaga
    13021 ataatatgtt tatattagcc tttataatat gtacagttgt tatatttata gtatctacta
    13081 aaataaatcg aaaaagatat gatgaattaa atttgatgtt aaatcaaata ttggaaggta
    13141 aagaagttac ttatccagat acaaaggata caagagcgtc aaaaatttct caccaagtaa
    13201 aaaaaattaa ggatatgata gaaatagagg tagaacagtc aaagtcagaa aaagaagcga
    13261 ttaaaggttt aatatcaaat atgtcacatc aattaaaaac tccactttca aatataacca
    13321 tttattgtga acttctggag aatataaata tttccacatt acagaagaag gagtttttac
    13381 aaaagatgaa aaatgaaact tttaagattg actggctgtt acaatcctta tttaaaatga
    13441 ctaaattaga ggatggagtt atagaatttg aagtagaaga actactaatt aaagatactc
    13501 ttattcaaag tatcagtact atctttaata aagcagaagc taaaaatata aggataaatc
    13561 tcgaaccatt ttcagacata aaattagttc ataataaaaa gtggaccata gaagcaattg
    13621 taaatgtttt agaaaatgca ataaaatact caccatcaga tagcacgatt actatttctg
    13681 taataaaaat ggaactatat acgaagatta ctattaaaga tgagggaata ggtattgata
    13741 gtagagaact aaatgatatt tttaaaagat tttacagaag taaaaatgtt gaaaaccaaa
    13801 atggcacagg gattggtcta tatcttacaa gattaatttt agaaaaagaa aatggaaaca
    13861 ttatagtaga atctaaacta ggaagtggat gttgttttag tatcttctta cagaattgta
    13921 agagtttaaa ctaattttgt aggatgagtg taattatcgt cacttacaat attttataga
    13981 acatatatct gtattgaaag gagaaattaa atgagtatat tagtgacgaa taatttagta
    14041 aaacactatg gaaaaggtga gactaaagta aaagccttaa atggagtaag tatagaaatt
    14101 gaacgagata catttactgc tatagttgga acttctggtt ctggaaaaag cacattgtta
    14161 aatattatag gtggattaga taatccaacc tcaggtgatg taataattaa aggaaagaac
    14221 atatctaaaa taggaaaaaa agatttgaca gtgtttagaa gaagaaatat aggatttata
    14281 tttcaaaact acagtttaat gcccgtatta aacacttatg ataatatagc tcttcctgtt
    14341 acattagata aaggaaacca tatagaccat gaatatattg aaatgctgat gaagacattg
    14401 ggaatttggg ataagcgttt aaaatttcca agcgaactat ctggtggtca acaacagagg
    14461 gtagcaattg ctagagcttt ggcaaataaa cctgcgctta tattagcaga tgaaccaaca
    14521 ggaaatctcg atagtaaaac tacaatggaa gtggtatgtt tattaaaaga aagtagtgca
    14581 aaattccatc aaacgatact tatggtaaca cacaatgaaa atatagcaca agtatgcgac
    14641 tcaattatcc atattgaaga tggagttgtg gtgaatactg gtggtgaagt actatgatgt
    14701 ctctagtaaa attttcaatt caatatataa agcattataa aaaacaatct atatctatag
    14761 ttttaagtat agtactatca gtagcattat tgacgggaat tggttcttta gttcatagtg
    14821 cagacaaaag tagaattgaa aagataaggg aagatagtgg agattatcat tatttctata
    14881 aagtagataa ggaacaactt aataaaatta aaaaaaataa aaaatcaaag gaatacactg
    14941 tagatagact tggcgttaca aaacctaaaa atcagataga tgaacctttt attatagaat
    15001 ttttaaatgc tgattcttca tatttagaca tgacaggtag aaaattatta aaaggaactc
    15061 ttcctagtaa agatggagag atagtacttg acacatattc tctaaacaat ttaaatgtaa
    15121 acaaagaaat tggaacagaa atcaatttag atggtaaaat ctataagtta tgtggtattt
    15181 tatcaagtct ttcaaaccca gaaactgctc tggagggatt tgtaaacgat tattttcagc
    15241 ctaaagaaaa ggattcttat atagtttatg taaaatatga tgaaaataaa aatattaaaa
    15301 agcaaaatta taaatttatg aaaaaattca acatatcaaa agaaaaaact tatataaact
    15361 ggcctctttc agttgaattt ggagtaaaac caccaaaaaa tatagaaaaa tataatatat
    15421 ttgagttctt aaaaaattta gaactaaata caaatgcaat tatattactt gttggagtgt
    15481 ttggtgcagc agcagtatat agtattttcc atgtttcaat acttcagaga atatcacaat
    15541 atggggtttt agaagtatta ggcgctaata ataaacaact tttatttcta ctatttttag
    15601 agttattttt actttttatt gttggatttc ctataggttg cttcttaggt attgaagtgg
    15661 cgtctacaat atatgaacaa tttccacata tatttttgag tagtgatatt gttccaggag
    15721 cattttttat aagtgaaaaa tctatttatt taggcttttt atttatctta ttgttgttaa
    15781 ttttaattgc tgtacgtgtt gtacatcagt tgaataaata ctctagtata gaatctatga
    15841 aaaactttaa gattactaat aagaaaaaga gaatattaga aagtgtaaaa cactctaaca
    15901 tgacaaaagt attatctcat aaatacatga ctcaaaaaaa gggaatgttt attggaattt
    15961 tgttttcatt atcacttggt ggaattatat ttttatgttc tagttactct atacagctta
    16021 ctaaaaataa taatgaactt acaatgaaag ctgatgatgg tttaaattct gactaccaaa
    16081 taactatgca aacaacagat tttgatgtag gtataccaaa agataaaatt tcaagattaa
    16141 acaatgttga gggaatatca aaagtctatc cagtaagata tttatttgga gctatgcaaa
    16201 ttaaagaaga acaactattt tggaaaaatt tctttaaacc tcttgaaaaa gattacagga
    16261 taaagacttt ttttgatggt atttgtacaa agcaaaatga aggtggctat cttttaaaaa
    16321 caaatattta tggttataac aatggtatgt tagaaggatt aaattcttat attatagatg
    16381 gggagattaa tacccatgat atggttaaaa ataataaggt tatagttaga ctacctatgg
    16441 atggaactgg gatgtatgat gcagttgata taaatccagg agatacaata aaagttaaag
    16501 tgccaaagac aatgaaacca acagatgaaa ttgttaaatt taaagaagaa aataagaatg
    16561 attatacaac aaaagaattt gtagttgctg ctactgtaaa acgtgtgatg gcaaataaca
    16621 tatatttcat tggtgattat ggtatggata tagttatgac gaatgggcaa atgaaaaata
    16681 actttgatat cgaaaattat aattcagtaa gtataaaaaa gacagaagat agtaacagtg
    16741 aggaattgtc tgagaacata aaaagtgttg taagtgatgt aaaaagatgt atcgtaacag
    16801 attataccat tgctattgaa aaaaataata cctacttaaa tcaaaaactc ttatttatat
    16861 atggtattgt atttgtactt cttgcaataa gtttgttcca tataatcaat acagtaagct
    16921 acttaatttt ttcaagaaga catgagtttg gaattttaag agctatgggt attacggata
    16981 ataagttttt acttatgatg ataagagaag gttttttata tggattctat gcaagtatta
    17041 taatggtaat tggaagtgtt attggacaat ttatgattta ttttatggta aaacgtgtat
    17101 atttatatat aaatccaatt cttaaaataa atacacctct atacataggt atgattattt
    17161 taaatatcac tattagtata gttgctgtaa ttattccagt aagacaaatt ttgaagagtg
    17221 atattatttc agaaataaat aagaattaga gttatataaa tttttaagat tatataactt
    17281 tctgacaatc ttgtttaaat tatttaaata tatcttatct gtgaacatta aataaacttc
    17341 ttaatagata taactcttga aaagtcatat ctattaagag gttttaataa attttcatgt
    17401 taatatatat aatccaaaaa aattaaatct tattaaaaca tactattttg tatagttatc
    17461 gaaatatcct tgtatgtaaa taataggagt acccttatct ccacttccag atgttaaatc
    17521 tgctaaagaa cctataaggt ctgttaatct tcttggagta gtaccttgag aaaccatgtt
    17581 tcctgttaaa tcatcagatt tattatcttt ttcaactatg tattttgaaa ttgcttcttt
    17641 taactcatca ccagataagt cagcaaaatc attatctgct aagtatttta attttacttc
    17701 gtttggagtt ccctctagcc cttttgtata agcaggagat actactgggt cagctaattc
    17761 ccatatttta cctacagggt ctttaaaagc tccatctcca taaaccatta cttctacatc
    17821 tttccctgtt atttctttta tattaccttg gattttgttt acaacttcat cacaatttat
    17881 agggaataat tttactgttt cttctgtagc tttgtttgaa cctaaaagtc catactggtc
    17941 attgtaacca ctaccatcta cacttgctgt cattatatca tcaagtgaat atactttttt
    18001 agctccattt tgtcttaaaa ttctctttgt tctttgtctt gtatgtatat cacaagttaa
    18061 aatacttgtt gtatagttaa gaatagtttt tggattgttt gcaaatacta tttctacctc
    18121 tgcaccacaa tccttgatta aattcttata ataatcaaca taatctacac cagtaaatgt
    18181 atgcttgtta tatccaaata gttctctgta tttttcttct gttaaaacat ctgaccaagg
    18241 atttattccc ttatcatcaa gttcatcaat acttattaag tgatttccaa cttcatcaga
    18301 agggtagctt aacattaata cgacttttct acatcctttt gcaattcctt ttaaacatat
    18361 agcaaaacga tttctactta aaattgggaa tataactcca acagtatcat ctccaaattt
    18421 atcttttaca tcttttgcaa tattgtcaac gtgagcataa tttccctgtg ctctagctac
    18481 aacagcttct gtaacggcaa ttacatcttt gtccctaact tcaaagtttt cactttttga
    18541 cgcatttaaa acagaatcaa caactatttt aactaagtca tctccttgtc ttataatagg
    18601 tgctctaacc cctcttgaaa cagttccaac taatctatcc attatctaaa atcctcctaa
    18661 agaattaata ttgtcatatt agtaccatgt atattataat tcaaatataa ttataagtaa
    18721 aataaataat actaatattt gacataagga gtaattatat atgaatataa aacttgaact
    18781 ttataaggtt tttaatgcag ttgtaaataa taagagtttt tctcttgctg ctaaggaatt
    18841 gtttatgtca cagccagctg taagccagtc aataaagcaa ttagaagaac aattagatac
    18901 tttattattt tatagaaaca ataaaggagt aaaattaact ccagagggga aaattctaag
    18961 tgaacatgtc actactgcat taaagttaat atcatcagga gaagatagaa taaataaatt
    19021 taaaaaatta gaatacggtt ctctgaaaat aggagtagga gatacagctg cacgtttttt
    19081 cttgttaaaa tatttagaaa tttttcataa gaagtatcca catatacatg tatctacaat
    19141 aaatagaacg agtagagaac ttatatcttt actcaaagat gggaatatag atatagcaat
    19201 tataaacatg ccaatagaag atgatacact aaatattgtt gagtgtattg aaatacatga
    19261 tatatttgta tgtgcaaatg attacataga atacaaaggt aggaaaatat cattggaaga
    19321 attaaacaca ttgcctttaa taatgctaga aaataaagca aattcaagac tttatgtaaa
    19381 tgaatatttt ttatcaaaag gtataaaact aaacccagat atagaattgg gttctcatga
    19441 attactttta gaatttgcgt atataaatct tggagtgtct tgtgttatag aagaatttag
    19501 catagattat ttggaaaatg agaaattgtt taaattagat ataaaagagc caatacctaa
    19561 gagaaatata ggttattgcc atttaaaaga tatatcttta tccttagcaa ccaaagaatt
    19621 tttaagcatg atatctaata atatataatt tagaatacat atttcacaag taatttaata
    19681 taaaaataga gataaattcg taaatttgaa agattcatat agtaattagt ttttattaaa
    19741 aataaaaata aggttaattt gagaacattt atataattaa tatcgtattt taaatatgaa
    19801 cagtatattt agattaatgt ataaaggggg attagaaatg tctgattctg aatatcctca
    19861 aatgaaaacc ttagaaacta gaagttgtat actaagacct gcatcattag atgatgttga
    19921 agattttttt gactgttata aaaataaaat agtagtaaaa catttaccat ttagtgagca
    19981 taaatcttta gaagatacaa gaaagtttat aaaatcattt tttctaaatc catataaaaa
    20041 agggaaaatt ggccactttg ctatagtata taaaagagac aataaagtta ttgggaatat
    20101 ggggtttaat aatataaatc ctaaagcatt agaagcagaa ataggtatat gtataaatcc
    20161 aagctattgg ggacatgatt ttgctactga gataacaaaa agagtaatac agtatggatt
    20221 tagggaacta aacttaaata aaataattgc aataacctat gaagaaaacc aaaattctac
    20281 aaaatcatta gatttattag gatttaaatg tacaggaaag tatatcaaaa aaattcatac
    20341 tggaaatact gtaaaaaatg ttccttgtta tcaatatgaa ttgaaaaaac aatagatata
    20401 atcatttaat atctatgctg gttatttagt aatgattgtt ttctaaataa aatttcaata
    20461 aagtggttgc ctcaaaatat agataatcct gtttttattg ttaaaatact gaaaatgcat
    20521 ttgattttat acaaaaaaga gtgtctcatg aactaaaaag tttatgagac actctttttg
    20581 aataaatcgt attttaatta aaatagtata tatacttaaa atattaattt ttattagttc
    20641 tctaaatttt taaatccttc tccaagaacc tcatgtacat ctgtaacaat aacaaatgca
    20701 gtagggtcag ttcttttaac taatctcttt agataaagtt cttgtttttt agaaactacg
    20761 actaatagaa cttctttatt ttctctagtg tatccacctt taccatcaag aatagtaaga
    20821 cctctatcaa gttcttcagt tatagcttgt cttaaacttt caggctcatc agagataata
    20881 aaaaatgctt ttgaatggtc aaatccttcc attatcatat ctgctatttt tattaataca
    20941 caaagtgcta taccagagta aagagcagtt tctaaactac gatttactat accagaagat
    21001 ataacaacca ttccatctag gcatgacatg aattttgtag cttttattcc tgggaatttc
    21061 ttactaagta tgagtgcaat taagtctgtt cctccagtag aagcatctga tttaaacatt
    21121 attcctaaac ctataccaac taggattcct cctgcaatag aagacaataa taagtcatta
    21181 gtaaatctta gttttgataa tgggtctgtt aattgaacta tagctgaaaa tacaactgtt
    21241 ccaaataaag tttttaatga attttttgta cccataattc taaaagcaag tattactaat
    21301 gggacaccta taattaacat gatagctgat actggaagtc ctgtaacctt gtttagtact
    21361 aatgatagac cactaagtcc tccaggagca attgtatgtg gttttaaaaa catatttata
    21421 ccaatactca ttgatataca accaaaaaat agtccaagca tttcaagtaa aattggcgac
    21481 ttttgttttt tcatataaac acctccatga acttgtcaca atttaatatt ataacatatt
    21541 tgaccagttt atattatata aaatttcttg atataagcaa tttcaagtat ttcattattc
    21601 ttaatatttt tgaattttta tatagtgttt tcatatcttt actgatgttg ttacactcct
    21661 atgaagtttt ttatatatta taattttttt attcttttat aaagtatccc ctataaagat
    21721 ggttctatga aaattttctc tatattgttt aacgtatata gagattttgt gtataattat
    21781 aaggtattta atggaaaaat ttatcttact tttatatgtt atcaaaatga tttattaaat
    21841 acaacagaca gcttgataac atgtaattat ataaatataa tttatgtaga tagttgtatt
    21901 tttaaataaa tttttacata tcaataatta ttgtctattt tatataagca acaatcaaac
    21961 taaaataaaa gtttaagaaa agagttgatt aaatgcataa acctagttat aaaacagtat
    22021 ttcattcata cattattttt ttcatgattt ttatttgctt aatcctgaca gtaataggta
    22081 ttttcttatc acttattact attcagaaac caaatgggga atttattcgt agtgactggc
    22141 cgaaaaaatt tacagaaaac tttaatgaac aaatcgtatt tatagataat aagccacaag
    22201 taaaacagtc tggattagaa aaacttcgag aaaatcaatt gtggttgcaa attttagatt
    22261 ctagtggaaa tagagtttat gggtttttag aaccaaaaga gcataaagat tattattcta
    22321 attctgaatt attagcatta gctaaccaaa ataattcaag taatggaact gtatacttta
    22381 atacaattaa aaataataaa aatgactata tttatattat atattttcca ttaaatatat
    22441 ctaaagtgaa tatgtttttt gatggaggta aatttatagg tggtaagtca attgtatttt
    22501 caatcatagg gattatattt ttaataattt tggtttctgg aattgcttat ggttactggg
    22561 taacaaagtg catatcaaat attacatctg ctgttagtga tatttcaaaa cgaagctatc
    22621 ttccagttaa tattaaaggt gtttttgctg atatatataa tagtttaaat attttaaatg
    22681 aagaaattca taaaagtgat gagatacaaa ataaaactga tattatgaga gaagagtgga
    22741 tttcaaatat aactcatgat ttaaaaactc cactttcacc aataaaaggg tatgcagagc
    22801 ttcttacaga tagtagtact aattattcac ctgaaaaagt atataaatat gcttcaatca
    22861 tgttaaagaa catagaatat acaaatacac ttataaacga tttaaaatta acctatcaat
    22921 taaaaaatgg aataatccct ttaaataaaa ataattatga tttagtaaga ttcttgaaag
    22981 aattatccat tgatatttta aattatccag aatatgaatc aaatatagtt gaatttaaga
    23041 gtaatataga taatattaac tttgattttg attgtacact attaaaacga gcttttagta
    23101 atcttattgt caatgctttt acatatggag ataaaaatac taaagtttgt gtacatgtaa
    23161 cattaaatga aagaattgaa atcattatat ctgataatgg aaaagggatg tcaagagaag
    23221 aagcatcaaa tttgtttaat cgttattata gaggaagcaa tacaaaacaa aaaatagaag
    23281 gaactggttt aggactagca ataacgaagc aaattattga agttcatgga gggaatatat
    23341 ctgttgaaag tatacctgga ttaggtagct cattttatat cacctttcct atttaattaa
    23401 ggttaattaa ggttaaataa aaaatatatt aaggttgata gattattata tttatatagt
    23461 agtttatcaa tcttttttat ttaaggggtg atttgattaa aaatattata aatagaatga
    23521 taaaagtatg gaggagtcta ttagagattt aatttagatt aaagtataaa aatattagat
    23581 tgaaatatct tagataaggg aagaaggtgt aagtttgaaa attatattaa agtatatttt
    23641 cacaaatatt aaagaacgaa aaattagaac agcagttatg ttattgtcca tagttttatc
    23701 aacagtttta ctttttgttt cattctcaat tggattatct tatgaaagtg cacaaagaaa
    23761 aatggcaaaa ggtatgtatg gtacagcgac tatatctgtc caaagtaaaa atccagatat
    23821 attaaccaat ttagaagaca tacctgattt aaatactata aaatcaaagg ttggtgtatt
    23881 ggaaagctct gctatttata ataaaggggg atactatgaa gaatttagtc ttatatcagc
    23941 agacttaagt caactaaata aaatcaacaa gccacgatta gaaaatggcg atagtattac
    24001 tgatttttct ggtgataaaa ttattcttcc aaatcgcttt acatcaaaat ataaaataaa
    24061 aaaaggtgat tctattactt tgcagattta tggaaagtct tatacttttc aagttagtga
    24121 tattgcatct tatgatacag tatttttgag gaacacaaga ggtgtaaatg cactgctacc
    24181 caaagagact ctatcaaaaa ttataaataa aggcagtgga tatacaagag ttcttataga
    24241 gtcagaagaa gaaattacag aaaatctagt aaataaatta tccgaagaac tttctactga
    24301 aaaatatatg gtatcaaata ctataaatga aactaagata atttcagatg ctagacaaaa
    24361 gacaatgcct ttctttttaa taagtttttt tgcattaacc cttagtatat ttataatata
    24421 cagcagttat aaggttatta cattagaacg tcttcctttt attggaacat ttagaagtat
    24481 tggtgccaat gaaaaaactg taactcgtat attgatgctt gaaagtatat tatacggaag
    24541 tataggagga ttaattgcta ttcctattgg tgtagttgtt ttaaatttaa tgcttcatgg
    24601 gttaggaaat tctctcgaac aaggaatatc aattccagta gtaatatcac ccattggagt
    24661 gattatatct gttatagtag caataatcgt ttcttcattt agtgcatata tacctgttaa
    24721 aaaagctagc catttgccga taaaaaatat tgtactagga acagtagaag aaaagaatgt
    24781 ttcaaatcgt tctatacttt ttataggaag tataatgttt attttatcta ttttacttcc
    24841 aagaatatcc cctgaaaata cattgtatct agctggtgga ttttcattac taggattaat
    24901 tgtagctact attgtgttga ttcctcttat tactgatatc atgtctatag tttttgagtt
    24961 tgtttataaa aatattttag ggaatgaggg taaattagct gctagaaata tgaaaaataa
    25021 caaaaatata atacagaata tcactttact atttataagt atttctgcag ttattgcaat
    25081 aagtgttgtt ggtaactttg tcaaaactta tataacagat gtgtttcgtg acgcagaatt
    25141 acaaggtttt gcagatggaa atatgaatga agagtttatt gaagatgtta gacatatgga
    25201 aggaattaaa aaaatattac ctctttatgt tatgaataat gagatttctg gtaatggtgt
    25261 aacattatca agactagagg gtacagataa tataaaacta tataactcta tgtttggaat
    25321 caattataca aattttgaga taaaaaaaca agtaatagaa gcttttaatg ataaacgttc
    25381 tgttattttg aatgaagata ctctaaaaaa agtaggctta tctataggag atacaataac
    25441 tctatctaat gataagtatg atttttctta taaaatagta ggtagtttta aatcccgtgc
    25501 aaatgatgta gaagctgtta taccatcaca ttatgcagtt aatgattttg ataaaactaa
    25561 ttatggtttt ttagtatata cagcagtcaa tccagatgct gttatgattc agataagata
    25621 tttatttgga gatacatata attggagtcg tacagttgaa gaatttaata atgattcttt
    25681 aaatactata agtagttttt tgtctcctat gaataagatg acctatttca tttttcttct
    25741 tgctacagta ggtattatta ataatctact gattaattat attcaaaaaa gacgttctat
    25801 agctatgtat aaatctatag gactaagtaa taaacaaaat ataaaagtaa cattgattga
    25861 aggggttact tcaggtttac ttggagctgt tattggtatt gtgatatcta tacttgagat
    25921 tcaaactatc tttatagttg caggaccaaa aatttcaatg aaaccagatt tagattttaa
    25981 aacatttatt attgttgggt tattgggaat tatagtaact ttaataggtt cgatagtgcc
    26041 tattataaaa gggaagaaaa tgaaactgat agaagaaatt aagtttgaat aattatggag
    26101 gttaatatga aaattacaca taatagttta gctgtagaag caaaaaatat tatcaaagaa
    26161 tataaaattg gaaatacaac aactcgagtg ttaaaagagg tatctttaca agtaatgaaa
    26221 ggtgaatttg tttctatcat gggtcaatct ggttctggta aaagtactct tttatatatt
    26281 ttaggtggtt tagatactcc aacgagcgga aaagtgtata tgaatggagc tgatatatca
    26341 cattttaatg atgagaaaat gagcataata cgtcgtagaa atataggatt cgtatttcaa
    26401 ttttataact taataccaaa tttaaatgtg gaggaaaata taatgcttcc tttgttatta
    26461 gatggtaaaa atttaaaaga ttataaaaat caattagatg aaatcttaga tatagttggt
    26521 ttaactgata gacgtaaaca tacaccacga gagttatctg gtggtcagca acaacgtgtt
    26581 gctatagctc gagctttaat tggaaaacca gagattctat ttgctgatga gcctactgga
    26641 aatttagata gtaaaacagg gatagaaata atagatttac tgaataaaat aaacagagac
    26701 aatggacaaa ctataattat ggtaactcac tcaccagaag cagccaaaag tagtagtcgt
    26761 actattacag tttcagatgg tttaattgta taaggactaa ttattgtatt tgagataagc
    26821 atttttaatt atataaatta gacatatgta aagtggatat aagttaaata ctataaatgt
    26881 gctataatat taaaataaca gataaaagta ctatattttt tattgattat tttaatttta
    26941 tttgtttatt aaatatctaa tgagtattca tactatctct attattaaaa gcagttaatg
    27001 atatatagag ataatatgga ttcttcttac tttaataaat attcacgata ggacaggata
    27061 taatatatgt taaaaaaaga aagtattcat tttataaata aaaataggtc acttatagtt
    27121 tgtgaagaca attttgttta tctcttgcct tgtatacaat taagaaattg gatatcgaat
    27181 tttactatat cgtttccaaa tcaaacaata atttctgata attacacgat tattcctcat
    27241 ggaagtgtaa ctttggtgtt tttttatgat gccacagggt tacatagtct attatttggt
    27301 ccaactacta aaccaaaaac agtaggaaat attgctaata gatgtgatgt tatactaatt
    27361 atagaatttc aacctgctgg ttttttcccg cttataggta tacagcaaag tgaacttatt
    27421 gataaagttg ttccattttc tataattaat acttcattag atttagaaat taaaaaaata
    27481 tttaatgaat ctttatctat agataaattg attttaaagt tggaagaatt attgatttct
    27541 aatattaaaa ttgaatactc atatgagttt attttggcaa ttcagttgat tattcaaaac
    27601 tctggaaata tatcttcaca agaaatttct aaaaaagtat tttatagttc taggcattta
    27661 aacagactct ttaatcagta tctagggttg agtatgaagt cattttctcg tttggtacgt
    27721 attaataaat ctataaaact tctaaacaat aataaaactt catggatgtc tatatgtaat
    27781 gaattaggat tctatgatat accacatttt attaaagatt ttaaaattgt ctgtggaata
    27841 actcctcaaa aataccgagc taatatgtct gatttttaca gtgaaattgc aaaattttaa
    27901 gttataatac ttataacaaa gaaaggatag tgataagatg aaatttggag gagtattgct
    27961 aattgtcaaa gacataaata aatcaaaaaa gttttttgaa gatgttatgg agcaaaaaat
    28021 cacattagat ttaggagaac atgtatcatt tgaaaatgga tttggattac aaagtaatta
    28081 taaagctata attggatatg atttaccaat atatcaacaa tcaaataact ttcagttgta
    28141 ttttgaggtt aatgacatca aacaatggga aagtaagata aatagaatag gaaatattga
    28201 atttttgcat tatataaaag aatacccatg gggtcaaaga acttttaggt tttatgattt
    28261 tgataaaaat attatagaaa tttcagaaag tatggaaagc gtcataaaga ggcttttaaa
    28321 acaaggttta gctttagaag aaatttcaaa acgcactatg tatccagttg aatttattac
    28381 acaatttcaa caagtataaa atattaaaat atataatttt ttctttatat tgcaaaaaac
    28441 aaaggcttgg ggcataatag aaccagaaaa acctgaatct caacagcaaa tggctcatat
    28501 ggatttcgct gttaaggatt tagaagaagc agttcaatat gcaattcatt gtggagctac
    28561 aatcgcagag gagcaattta ctgatgactg gagagttatg attgacccag ctggacaccc
    28621 tttttgctta tgtcagatga aatcaattat ggaaagtagc catttttcat tgttatagac
    28681 caaaatattg tttattaatt acaaaaaagc acaacagtat ataccgttgt gcttttactt
    28741 attatacatg aatacactaa aaaatataaa tagtagagct ttatcaataa tgataatttt
    28801 taaaaaatag tattttttta ttgaataatt tcttagatat tttaaagtat ataaattttg
    28861 catatatgaa ttggacatta tcaaatgtgt tat
//
LOCUS       DAYAOX010000024        28863 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.024, whole genome shotgun sequence.
ACCESSION   DAYAOX010000024 DAYAOX010000000
VERSION     DAYAOX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.024"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            224..1510
                     /locus_tag="ACGI57_000890"
     CDS             224..1510
                     /locus_tag="ACGI57_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFG9715976.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1622..2095
                     /gene="grdA"
                     /locus_tag="ACGI57_000891"
     CDS             1622..2095
                     /gene="grdA"
                     /locus_tag="ACGI57_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_110943085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1754..1756,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HFG9715977.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2127..3437
                     /gene="grdB"
                     /locus_tag="ACGI57_000892"
     CDS             2127..3437
                     /gene="grdB"
                     /locus_tag="ACGI57_000892"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3174..3176,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HFG9715978.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3693..5225
                     /gene="grdC"
                     /locus_tag="ACGI57_000893"
     CDS             3693..5225
                     /gene="grdC"
                     /locus_tag="ACGI57_000893"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HFG9715979.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5236..6381
                     /gene="grdD"
                     /locus_tag="ACGI57_000894"
     CDS             5236..6381
                     /gene="grdD"
                     /locus_tag="ACGI57_000894"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HFG9715980.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6691..7770
                     /locus_tag="ACGI57_000895"
     CDS             6691..7770
                     /locus_tag="ACGI57_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFG9715981.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7883..8482
                     /locus_tag="ACGI57_000896"
     CDS             7883..8482
                     /locus_tag="ACGI57_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715982.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8668..9540)
                     /locus_tag="ACGI57_000897"
     CDS             complement(8668..9540)
                     /locus_tag="ACGI57_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9715983.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            9957..11360
                     /locus_tag="ACGI57_000898"
     CDS             9957..11360
                     /locus_tag="ACGI57_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715984.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            11387..12931
                     /locus_tag="ACGI57_000899"
     CDS             11387..12931
                     /locus_tag="ACGI57_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HFG9715985.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            12962..14353
                     /locus_tag="ACGI57_000900"
     CDS             12962..14353
                     /locus_tag="ACGI57_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFG9715986.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            14546..16042
                     /gene="abfD"
                     /locus_tag="ACGI57_000901"
     CDS             14546..16042
                     /gene="abfD"
                     /locus_tag="ACGI57_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HFG9715987.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            16177..16452
                     /locus_tag="ACGI57_000902"
     CDS             16177..16452
                     /locus_tag="ACGI57_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9715988.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            16476..17783
                     /locus_tag="ACGI57_000903"
     CDS             16476..17783
                     /locus_tag="ACGI57_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HFG9715989.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            17803..18921
                     /locus_tag="ACGI57_000904"
     CDS             17803..18921
                     /locus_tag="ACGI57_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HFG9715990.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            19208..20128
                     /locus_tag="ACGI57_000905"
     CDS             19208..20128
                     /locus_tag="ACGI57_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFG9715991.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            20243..21400
                     /locus_tag="ACGI57_000906"
     CDS             20243..21400
                     /locus_tag="ACGI57_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFG9715992.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(21485..22984)
                     /locus_tag="ACGI57_000907"
     CDS             complement(21485..22984)
                     /locus_tag="ACGI57_000907"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFG9715993.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            23580..25004
                     /locus_tag="ACGI57_000908"
     CDS             23580..25004
                     /locus_tag="ACGI57_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFG9715994.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            25030..27168
                     /locus_tag="ACGI57_000909"
     CDS             25030..27168
                     /locus_tag="ACGI57_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9715995.1"
                     /translation="MDNLKKKKITSRQKKIILMIVANSKKNIPITISEIAGTLELSSR
                     TVLRDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEER
                     NLIILSKLLVSNEPVKSYYFAKILKVSEWVLNNDFALASKWLERFDIELVRKPGLGVY
                     LKGQEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCI
                     IRKVEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITE
                     EFKLAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITN
                     KMIDLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYD
                     VVEKISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSR
                     FLSTKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSL
                     DDEQIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEK
                     FESKDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERID
                     IMKLSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKI
                     NKFSIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            27198..27620
                     /locus_tag="ACGI57_000910"
     CDS             27198..27620
                     /locus_tag="ACGI57_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9715996.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            27639..28790
                     /locus_tag="ACGI57_000911"
     CDS             27639..28790
                     /locus_tag="ACGI57_000911"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFG9715997.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
BASE COUNT        11290 a         3318 c         5402 g         8853 t
ORIGIN      
        1 ctgaagataa gaagaaaaag gtttttatgt ccaactttgt taaaaaaata attttaaata
       61 aaataatttt tttacaataa atatttttta gtaataaaat aaagatttaa ggcataaaaa
      121 cacaataata tatctttaaa aaaataagat aaattgtaaa atgatataat tttgagaaaa
      181 actcaaatgc atatacacaa attaaataac aggaggtgca agtatgcgtc ttgaattagg
      241 gaaaatcttt ataaaagata ttcaatttgg tgatgtaaca gaagtaaaag atggagtatt
      301 attcatcaat aagcaagaga tgctacaaga aattggtgga gatgagcata ttaaatctat
      361 agatatagaa ttagctcgtc caggtgaaag cgtaagaata acacctgtaa aagatgttat
      421 tgagccaaga gtaaaagttg aaggtaatgg gggaatcttc cctggtatca tgtctaaagt
      481 tgacactgta ggagaaggaa aaactcatgc tttaaaaggt gtagctgttg taactactgg
      541 aaaaatagta ggtttccaag aaggtataat agacatgact ggagaaggag ctaagtacac
      601 tccattttct aaattaaata atgtagtagt aatagctgaa cctattgatg gtttaaaaca
      661 atacgctcat gaaaaagctg ttagaatgat aggattcaaa gcagcaatgt acttaggaga
      721 agtagcaaga aatcttactc cagatgaagt ttcagtatat gaaactaaac ctcttcttga
      781 atcaataaaa gaatatccag agcttccaaa agtaggatat gtttacatgc ttcagactca
      841 aggtttatta catgatactt atgtatatgg agtagatgca aaacaaatag taccaacttt
      901 attatatcca acagaattaa tggacggagc aattgtaagt ggtaactgtg tttctgcatg
      961 tgacaaaaac ccaagttatg ttcatataaa caatggggtt gttgaagact tatatgcaag
     1021 acatggtaaa gatataaact ttgtaggtgt aattataact aatgaaaatg tttatctagc
     1081 agataaagaa agatcttcaa actggacttc taaattatgt aaatatttag gattagatgc
     1141 agttatagtt tcacaagaag gttttggaaa tcctgatact gaccttataa tgaactgtaa
     1201 gaagatagaa atgcaaggtg ttaaaacagt tatagttaca gatgaatatg ctggacgtga
     1261 tggaggttct caatcacttg cagatgcaga tgtaagagct gatgctgttg taacaggtgg
     1321 aaatgccaac caagttgtag ttcttcctaa gttagataaa gttattggac atttagaagt
     1381 agtagatgta atagctggtg gttctgatgg aagtttaaga gctgatggaa ctattgaagt
     1441 tgaaatccaa gctataacag gtgcaactaa tgaaacagga tttggtcatt taactgctaa
     1501 aggatattaa gaagtatttt attaaacgat tgagtaatgt taataaaaca tattatagat
     1561 aattataata agtgttatag atatataaaa atatattaga aatataataa ggaggattat
     1621 tatgagttta cttagtaata aaaaggttct tataataggt gaccgtgatg gtataccagg
     1681 acctgcgata gaagaatgtg taaaaacagt agaaggagca gaggttgttt tctcatctac
     1741 agaatgcttt gtctgaacag ctgctggggc tatggactta gaaaatcaaa acagagttaa
     1801 agatgctgct gataaattcg gagctgaaaa tgttgtgatt ttactaggtg ctgctgaagc
     1861 cgaagctgca ggtcttgcag ccgaaacagt aactgctgga gatccaactt tcgctggacc
     1921 acttgctgga gttgccttag gattaagtgt ttaccacgtt gttgaggaac caataaaatc
     1981 attatttgat gaaagtgtat atgaggacca aataagtatg atggaaatgg ttttagaagt
     2041 tgaagaaata gaagaagaaa tgtctggtat aagagaagaa ttttgtaaat tttaatgcct
     2101 aaaagcaaaa ggggtgaaac taagagatgg gaaaactaaa agcagtacat tatattaatc
     2161 agttctttgc tggtataggt ggagaagaaa aagctgatac taagccacat gtggcagaaa
     2221 ctttacctcc aataagttta caacttgata aattattagg tgaagatatt gaaatagttg
     2281 gaacagtagt ttgtggagat agctatttca atgaaaatat agatagtgca agtgaagaag
     2341 ttttatcaat ggtaaaaggt tttgaaccac aacttttcat agctggtcct gcatttaatg
     2401 ctggtagata tggggttgct gctggaacaa taactaaagt tgtaaaagat gctttgaata
     2461 taccagcttt aacaggtatg tatgttgaaa atccaggagc agatatgttc aaaaaggatg
     2521 tttatgtagt agaaacttct gattctgcag caggtatgag aaaggcactt cctaagatag
     2581 ctaaacttgc agtaaaactt gctaatggag aagaaattgg aactcctaaa gatgaaggat
     2641 acatagctag aggaataaga gttaactact tccatgaaga cagaggttct aagagagctg
     2701 ttgacatgtt agttaagaaa ataaaaggtg aaccatttga aactgaatac ccaatgccaa
     2761 actttgatag agttgaccca agtaaagctg taaaagattt atcaaaatgt aaaatagctt
     2821 tagttacttc tggtggtata gttccaaaag gaaatccaga tagaatagag tcttcttcag
     2881 cttctaaata tggaacttat tctatagcag gagttatgga tttaacagaa gagacttatg
     2941 aaactgcaca tggtggatat gacccagtgt atgcaaattt agatgcagat agagtattac
     3001 ctgttgatgt tcttagagat ttagaaaaag aaggtgtaat tggaaaatta catgagactt
     3061 tctatacaac tgtaggtaat ggtacatctg ttgcaaactc taaaaaatat gcttctgaaa
     3121 ttggagcagc attagtggct gatggtgtag atgcagtaat attaacttct acatgaggta
     3181 cttgtactcg ttgcggtgca acgatggtaa aagaaataga aaaaactgga cttccagtag
     3241 ttcatatgtg tacagtagta cctatttcat taacagtagg ggctaataga atagttccaa
     3301 ctatagcaat acctcatcca cttggaaatc cagcgcttga ccctacagaa gaaaaagccc
     3361 taagaagagg ccttgtagaa aaagcattaa atgctttaac tacagaagta gatggtcaaa
     3421 cagtatttga aaaataaaaa taacacacac atatattata tagataaaaa tataaacaca
     3481 cactaaaaat aaaaaataac tatacacaaa aaaataatat agatttcatg aatggataaa
     3541 ttggagttat tcattcatga aatcttaaaa aatgccaaaa tacataaaca ataatagaat
     3601 caaatataca ataaaataca atgattagag cttttaataa gtttaataag attaaaaaat
     3661 aagtgttcat aaaatttatg gaggtgcaca aaatgactta tccagtttta aaaggggcag
     3721 gctatgtact tattcatact ccagatatga tagtgcaaaa tggaagtact tgtacagttg
     3781 aaagagcaac aaatccagat tcagaatttt taaaagaagt aagtaatcat ataagaagtt
     3841 atgaagatgt agttaactac atgccaaatc aagtttatat aggaaataga agaccagaag
     3901 aattaagaga tttaccaatg ccatggtgtg aacaaaagat tgagggaact agaaatggta
     3961 agtttggaga aataatgcct caagatgagt tcatagcttt aatgcaaata agtgatgcat
     4021 ttgatttagt aaaattatca caagaattta ttgatgaagt aaagccaaaa atcgaaaata
     4081 actaccctga aattgcacca tttgtaggta aattaaaggg tgatgatata gaagaaggta
     4141 aagaattagt agcaacacac atagctgaag gattatacca tgatggaaaa tttgttggtt
     4201 atgtaaaaag agcacatgat gtagatgtta acttaaatgc ccatacaatg tttgaaaatc
     4261 tagtagtaaa agcatctgga gttttatctg ctattcaaat gctaagacat agcaaaatag
     4321 accctgctga aatagattat gttattgagt gttcagaaga agcttgtgga gatataaacc
     4381 aaagaggtgg aggaaacttt gctaaatcaa tagctgagat agcaggactt caaaatgcaa
     4441 ctggttctga cacaagagga ttttgtgcag caccaactca tgcattaatt caagcagcag
     4501 ctttagtaaa agctggtata cataaaaatg taatggtagt agcaggtgga gcaagtgcta
     4561 aacttggaat gaatgctaaa gaccatgtta aaaaaggtct tcctgtactt gaagatgttg
     4621 ttggaggatt tgcagtactt gtatctgaaa atgatggtgt aaatccagtt ataagaactg
     4681 atttaacagg taagcatact gttggaactg gttcttcacc acaagcagtt atgacagcac
     4741 ttataacttc aggtcttgat agagctaatt taaaaataac agatgttgat gtgtattcag
     4801 ttgaaatgca aaacccagat ataactaagc cagcaggagc aggagatgtt ccagaagcaa
     4861 actacaaaat gataggggca cttgctgtta aacgtggaga cttagaaaag aaagaattga
     4921 aagattttgt ttctaacaaa ggattaccag gatgggcacc aactcaagga catataccat
     4981 caggagctcc ttatatagga tttttaatag atgatttaac tacaggaaat agaaatagag
     5041 ctatgatagt tggaaaaggt agtttattct taggaagaat gactaactta tttgatggag
     5101 tatcttttat agctgaaaga aatacaggtg ttactgaaga gacttcagga atatctaagg
     5161 atgaaattaa gaagataata gctgaatcta tgaaaaaact agcattagat atgttagaag
     5221 aataggggtg aggatatgtc aaaaaaagta atcgcagatg tattcttaga agtagctaat
     5281 gcaatagaaa gcggcgaatt tggcaaaaaa gtaaaaattg gtgttactac acttggaagt
     5341 gaacatggtg tagaaaacat ggttaatggt gcacaattag caaaatctaa tttatttgat
     5401 attgtgctta ttggaccaaa agtggaaact gaccttgaag tagttgaagt taatgatgaa
     5461 aaagaaatgc atgcaaaaat ggaagaatta ttagattcag gatatattga tgcttgtgtt
     5521 actatgcatt ataattttcc aataggtgta tctacagtag gtagagtaat aactccagca
     5581 aaaggaaaag aaatgatact tgcaacaaca acaggaacaa gtgctactaa tagaatagag
     5641 gcaatggtaa gaaatgccat ttatggtatt gctacagcta aatcaatggg taacaaatgc
     5701 ccaaaagttg gaatacttaa tgttgatgga gcaagacaag ttgagaagtg cttaaaagaa
     5761 ttaaaagata atggatatga catggagttt gcggattcaa ttagagcaga tggtggatgt
     5821 gtaatgagag gtaatgattt acttgtaggt gcacctgatg ttatggttac tgatacatta
     5881 tctggaaata tatttatgaa agtattttct tcatatacta caggtggaga ttatgaagct
     5941 caaggctttg gatacggacc tggagttgga gaggactatg atagaaaggt acttatagta
     6001 tcaagagcat caggttctcc ggtagtagca aatgctttaa aatacgctta tgatgttgta
     6061 aagggagata taagtaatgt tgcaagaaat gaatttgcaa aggttaaaaa agcaaaattt
     6121 gatgacatta tatcctctct tactaaaaaa gaagttaaag ctgaaaaagt agaagttaaa
     6181 atgccagata aagaaattgt tactcgtcaa atagctggtg ttgatataat ggatttagaa
     6241 gatgcagtat ctgagttatg gaaaaacgga atctatgctg aaagtggtat gggttgtaca
     6301 ggtccaatag ttttagtaaa tgaagctaaa ggagacttag cagttgaaac tttagttaaa
     6361 gctggatata ctgcaaaata agtacatttt ttatatgtaa ataagaacat atagttataa
     6421 ttttattgat tataattata tgttcttttt attttatatc ttgtcaatat ttatatatta
     6481 acacaatcca tttatttgat tctctagcta atttattata tttattcagt taaaattaag
     6541 ctttctaact aaataaaaac taatgaagta ttaaaatgaa attgataaaa tatgaaaaaa
     6601 ttttaggaat aaagtatctg ttagataaaa ataagattaa ttagtgatat aatataatta
     6661 agataaaatt tattatttgg aggcaaaatc atgagcagag taaaaaatgt agtagaattg
     6721 ttggaaacta aaggtgttga tgcactatat ctaacaaaga aaactaatgt aaactatata
     6781 agtggttttc ctgatgaaga agcatatgca gtgatttgca aagatggaaa ctttttagtt
     6841 actgatagta gatatatgga gcttgcagaa aaggtttgca aagattttga aattataaat
     6901 tggcataatt ttgatagaag tgtggcaaag gcagttaaaa gtgtatgtga taaagtgggt
     6961 ataaagaaac ttgggtttga aagaactaat atagtgtttg ataaatatga agaacttaaa
     7021 aatctgattg agaaggataa tggagaatta atacctacag aaaatattgt agagactttg
     7081 agatatgtaa aagacaaaga tgaaatcaaa aatacaagaa aagcttgtga aatagcggat
     7141 aaggcattag aagaattgat tccacatata aaagctggag ttagcgaaat tgaactggct
     7201 acaaagttag agtactttat gaaaatgaac ggagcacaaa atatagggtt tgaaactata
     7261 ctgatatcag gagcaaagac ttcattgctt catggtaagc ctagtgataa gattatagaa
     7321 aaaggtgatt ttgtacttat agactatggc gctatgtata atggctatat atctgataca
     7381 actagaactt ttatagttgg aggagcttct gaaaagcaat tagaaatata taatttagtt
     7441 aaggaagctc aaaatgtagg ggttgagaac atgaaagctg gtgtacatgc gacaattcca
     7501 gatgctgaga taagaaaagt tgtcaaaaaa tatgaagact attactatca aggtatagga
     7561 catggagtcg gtagagatgt tcatgaagaa cctttcattg gtaattatgg tgataaaatc
     7621 atagaagagg gatgtataat aacaatggaa ccaggtattt atttccctgg atggggtgga
     7681 gttagaatag aagatactgt tttgattact aaaaatggtc cagaaagact tactaaattc
     7741 cctaaagatt tgatgatttt agacaaataa tttaatttat atatattttt ttacataaaa
     7801 tataacataa tctcactatt aagctctatt tacatattat agtaaagagg gcttaatttt
     7861 attgtgagag gtgatagtat atatgtataa aaaacataaa tttataaaaa atataaaaaa
     7921 agcctggtat aaaatcaaat taattgatag atgccttata attgtaatgg caatattaat
     7981 gtttcaatct atatataatt tatttgttaa tgaagtaaat tcacaagata ctacaactat
     8041 tgatgtagta gtaagaacca cttcagcagc tatatttggg tattttttaa gtgctaattt
     8101 tataaagaga cctttaagga agtatacaaa tactgatgta tcttcaaatc tttttataga
     8161 taataattta tccccaaaag gtcaagagtc taatcaaaat aatataatga atgtcaaaaa
     8221 taccattgga tttacttcag aagctgataa ttatgaaaag aaaataccta ttaataataa
     8281 tgaaggtttt gaagaaggag aaactagtga attgcaaatt attatagcta ctgtaatttg
     8341 tatagtagca ttaacagtac tttttgttgt aagaaatttt acaactacaa catcagcttc
     8401 tttaggtact atttcacaga tgagagattt tgtctcagga tgcgtgggtt ttttgctagg
     8461 ttgtccaagt aaacctgatt aaaaaagtat tatcaataaa aggtatttta aaaagtatca
     8521 gtataataat atttagattt aaatgtatac aattataatt ctaaacattc tatattgtat
     8581 ttttatactg atactctaaa ctatactatt tattaaaata ttttaacatt gtatttaaac
     8641 aaaatattat atctgtataa tataaattta gtttatatga atagatgcca tatactcaat
     8701 aaaaagtttc atactatgag atatgtattg atttcttctc caaagaaccc caatattcgc
     8761 agtaattgca cgttctaatg gtattgaaca gagattttct gtattttgaa atatttcttt
     8821 atacaatatt gttgaagcca agtcattttc tagtaaataa cgtatggtag aaagttgatt
     8881 tgagtgtaaa attatatttg gcttagcttt ttcaagtctt tttgtaataa tcttatagtg
     8941 ataagaacct tcagataaga ttacaaaagg tacatttttt aacatataat tcgatataat
     9001 gggatgtttt gccaattcat ttttgggatt tgtaactaag tataattctg ttttatagat
     9061 tgtctttgaa tctatatcat cataaatatc actgtcaatt ataccaatac aaaaatcaag
     9121 tgtagattct tcaatcattt tagctccaac aaatgttgga acttcaaaaa ttttaagctc
     9181 tatatctgga taaattacat taaatctagg aagtattttt gaaagcaaaa acgtacctaa
     9241 aactgtagga atacctattt tgagagaagc ttttctcttt tttcctaaat caagtgcgtc
     9301 acattgaaaa tcctcaaatt gttttataaa atgttttgac ttgtctaaaa aaatttttcc
     9361 ttcaggcgtt aactctattt tattacctat tctgtaaaaa agttcaaagc caagttcttt
     9421 ttctaattct ttaattgcta atgttatgga tggttgagaa atatgaatac tttccgcagc
     9481 cttagtcatg tttttatatt tacagacagt ttgaaaatag tataaatagt ttatattcat
     9541 ttggtattag ttcctttcat ttacatatta gtgacaagta tataattaac aatagcataa
     9601 gttttgtata ttgttgtaaa aaaatagaat agttttaaac tatcatagca taagtttttg
     9661 atattttcaa tctattaaga atagtgctat tattaaatta acaaaagaga atagctggta
     9721 tggtttagag taaatcaatc tatattacag ttatatcaac gaatatcaaa aaattatcta
     9781 ttagaaaata aaaattattt ttacaattaa attgttaatt aaatcataaa gaaaaaagtg
     9841 atattaacaa gataaaattt ttttaatcag aaatgttaat ttaataacaa aatagagtat
     9901 tggggagaaa ggagaaatat attttttaat aatatttgtt aatttaataa cgaaaaatga
     9961 aagggagtga tttagtgaag tttgaattga tggatttttt aatgaatcct tttgtcctta
    10021 tgtttgctgc agtaattaca ggaattttat ttggaaaaat taaatttggg aagtttaatt
    10081 ttggggtatc aggagcatta tttacaggat tatttattgg atggcttgca tatagcttgg
    10141 ggaatctaat aatagcaaaa ggggaaacag cagcaggata taaggcagca actgtaatga
    10201 tgggtaatgg aattatttct tctgacttct ttgatttctt tttaataata tttgttgcag
    10261 cagttggtct attggcagca aaagatatga aagctgtatt aaagaagtat ggagcaagat
    10321 ttgtaatttt aggagttctt ataactttta taggtggctc tatgacttat gcaatgactt
    10381 tactatcaag tgataaagga agtagtgctt atgaagtttc tggcgtttac acaggagcat
    10441 taacaagttc gcctggatta gctgctgctt tagaaacagc tggtaaacat gcagaagatg
    10501 tatcaaaaga atttgaaaaa gcttcaataa aagataaaaa agaaatttta aaagtagtcg
    10561 acccagaagg caaattggat gtgaatacaa ctacatcact gactcaagag caaatagata
    10621 agtatattgc atatgctgaa gctggtgttg gtataggtca tgcagtcgca tatccttttg
    10681 gagttttaat agtaatctta ggtgtaaatt tccttccaaa attatttagg atggacctaa
    10741 aagaagaaag aagaaaatat gagaaggaaa tgaaggaagc aagagatagt gtttctggta
    10801 aaacgatacc agaagttcca tttaatataa taacattttt cttgacatgt ttagctggat
    10861 acttagttgg aggaatacat gtatttatgg gaccactagg gtacttcaca ctaggcgcaa
    10921 caggaggttc actaattgta tctctagttc ttggatatat aggtaagata ggggtagtaa
    10981 atttcagaat ggaagaaaag gttttaaata ttttaaaaca aatagggcta gtatttttct
    11041 tagcaatagt tggattaaga tatggtggaa aagtagttga ttcaattatg acatctggta
    11101 tgcacctagc cttagtagca atagcagtag gtgttacagc tatgatgata ggattcttag
    11161 ttggcaaata cgtatttaaa ttaaattgga ttttattatc tggagctgtc tgtggtggta
    11221 tgacatcaac accaggtctt ggagctgctg ttgatgctct tgatagtgat gacccagcag
    11281 caggttatgg tgctacgtat ccatttgcat tattaactaa agttatattg gttatagttt
    11341 tacacaaact tccaatgtaa ataagatttt atatgggggg atatttatga ataatgaagg
    11401 agcatttgaa gaattaagtg gaagattaag aaataaagaa ttgtgtgatt taataatgaa
    11461 tgcagatgaa gctgctaaaa ttatagagga tggcatggtt attggagtta gtggatttac
    11521 accttcaggc tatccaaaag cagtaccatt agcagtttca gaaagagcta aaagtggaga
    11581 aaagataaaa ttaaccgtat attcaggagc atctttaggt ccagaagttg atggagcatg
    11641 gtcagaagca ggtataattg aaagaagact tccttatcaa actaattcaa tacttagaaa
    11701 taatattaat aaaggtgttg ttgattatat agatatgcac ctaagtcatt ctactcaatt
    11761 tttaaattat ggaactatac ctaaagtaga tgtagctata gtagaagcac ttgcaataac
    11821 tgaagaagga aatattatac caactagtgg gataggtaat tctccatctt ttataaaaag
    11881 tgcagataaa gtaatagtag agataaatct tgcaaaacca atggaaatgg aaggtatggc
    11941 agatatatat attacggaaa atccaccaaa tagaaaacca atagaaataa atcatcctaa
    12001 ggatagaatt ggaacaacat atataccttg tggtttggat aaaattgcag gagtagtaat
    12061 taccaatatg caagataaga cccgtccatt gggagttgta gatgaagctt cgaaaaaaat
    12121 atcaaataat attattgctt ttttaagaga agaagtaaag gcaggcagat tatcaaagaa
    12181 tttacttcct cttcaatctg gtgtaggaag tgtagcaaat gcagtattat atggattatg
    12241 tgaatctgaa tttgaaaatt taacttgtta tacagaggtt gtacaggact ctatgttgga
    12301 tttaataaga atgggtaaag ttacaatggc atctactaca tctgtaagtc catctccaga
    12361 aggattaata aaatttgaaa aagatattga tttttttaag gataagatta tattaagacc
    12421 tcaagaaatt agtaacaatc cagaaatagc cagaagaatt ggtgtaattg caatgaacac
    12481 tgcaattgaa gttgatattt atggaaatgt aaattctact cacataatgg ggtcaaagat
    12541 gatgaatgga attggtggtt ctggtgattt tgctagaaat ggagcaataa ctatatttag
    12601 tactgagtca attgcaaaaa atggtgatat atcatcaata gtacctatgg tatctcacgt
    12661 agaccacact gaacatgatg taatggtaat tgtaacagaa caaggatatg ctgatttaag
    12721 aggtcttgca cctagagaaa gagctataaa aataatagaa aattgtgccc atccagatta
    12781 taaagaacaa cttagggatt atttaaatag agcttgtaaa agtggagcaa aacaaacacc
    12841 tcacatattg gacgaagctt tatcatggca tagtaaattt atgagtacag gtacaatgaa
    12901 aaaggctgaa acttttaaaa gtgcattata aaatatttaa taaattggag gtagatttta
    12961 aatggaaaaa gcagtggaaa actttgaaga tttaagtaag gaatacatta atggttatat
    13021 tgagagagca agaaaagctc aaagagagtt tgaatgttat actcaagaac aagtagataa
    13081 aatagtaaaa atagttggaa aagtagtgta ttataatgct gaatatttag ctaaacttgc
    13141 tgtagaagag actggaatgg gagtatatga agataaggta gctaaaaata aaagcaaagc
    13201 aaaagttata tataacaatt taaaagataa aaaatcagtt ggtataattg atatagatag
    13261 agaaactgga ataacaaagg tagctaaacc agttggagta gtagcggcaa taactccttg
    13321 tacaaatcca atcgttacac ctatgagcaa tgcaatgttt gcacttaagg gaagaaatgc
    13381 aattattatc acacctcatc ataaagcaat tggatgtagt acaaaaactg tagagatgat
    13441 taatgaagaa ttagaaaaaa taggagctcc agaaaattta atacaaatac ttgaccaaca
    13501 atcaagagaa aatacaagaa atttaatttc atcagcagat gtagtaattg ccactggagg
    13561 aatgggaatg gtaaaagctg cgtactcaag tggaaaacca gcacttggtg taggggcagg
    13621 aaatgttcaa tgtatcatag atagagatgt agacattaaa gaagcagtac ctaaaatcat
    13681 agcaggaaga atttttgata atggtatcat ttgttcaggt gaacagtcag taatagtagc
    13741 agaagaaatg tttgataaaa taatggatga atttaaaaat aataaaggtt ttatagttag
    13801 agataaagta caaaaagaag ctttcagaaa tgctatgttt gtaaataaat ctatgaataa
    13861 agatgcagta ggtcaatcag ttcatacaat tgcaaaaatt gcaggtgttg agataccaga
    13921 agatacaaaa ataatagtaa tagaagctga tggaccagga gaagaggata ttattgcaaa
    13981 agaaaaaatg tgtccagtta tatctgcata caagtataaa agttttgaag aaggtgtagc
    14041 tattgcaaaa gcaaatttaa atgtagaagg aaaaggacat agtgtatcta tacattcaaa
    14101 tacagttaaa aatatagaat atgcaggaga aaatatagaa gtatcaagat ttgttattaa
    14161 tcaatgttgt gctactagtg ctggaggaag tttctttaat ggtcttgcac ctactaatac
    14221 tctaggttgt ggttcttggg gaaataacag catctctgaa aatcttgatt ataagcatct
    14281 aattaatatt tctagaattg cttattatat gccagaaaat gaagtcccta cagatgaaga
    14341 actttgggga taaaatttag acaaaaaata ttcattctaa attaaagtag gaatatattt
    14401 taatttagaa tgaatttaag acataaatta aatttaaatt agtttattgg actaaattta
    14461 aaatattaag agacatatag aagaaggaag tattataatt taaaataaat aaaaaaatct
    14521 ataaaagaga aggagtggtt ttattatggc attaatgaca ggagctcaat atattgaaag
    14581 tttgagaaaa ttaaatacta aggtttatat gtttggtgaa gaggttaaga attgggttga
    14641 ccatcctatg attagaccat ctataaattg tgtagcagca acttatgact tagctcatga
    14701 cccagaatat gcagatttaa tgacagttac ttcaaatatc acaggtgaaa aaataaatag
    14761 atttggtcat cttcatcaaa gtgttgatga tttgataaaa aaagtaaaaa tgcaaagact
    14821 atgtggtcaa aaaacagctt cttgtttcca aagatgtgtt ggtatggatg cgtttaatgc
    14881 agtgtattca acaacatttg agtgtgataa agcccatgga actaattatc atgataattt
    14941 tgtaaaatac ttaacttata tacaagaaaa cgatttagta gttgatggag ctatgactga
    15001 ccctaaggga gatagaagtc tatcacctag tgctcaacct gacccagata tgttccttca
    15061 tatagtagaa agaagagaag atgggataat tgtaagagga gcaaaggcac atcaaactgg
    15121 ttcaataaat tcacatgagc accttataat gcctactata agtatgactg aagcagataa
    15181 agactacgct gtatcatttg cagttccttc agatgctgaa ggagtgttta tgatttatgg
    15241 aagacaatct tgtgacacta gaaaattaga agaaggtgca gatgttgatt taggaaataa
    15301 ggagtttgga ggtcaagaag ctttagtagt atttgataat gtatttgttc caaatgatag
    15361 aattttctta tgtggagaat gggatttctc tggtatgtta gtagagagat ttgcaggata
    15421 ccatagacaa agttatggtg gatgtaaagt aggagtaggt gatgtaataa taggagcagc
    15481 agctttagca gctgattata atggagcaaa caaagcttca catataaaag ataaactaat
    15541 agaaatgact caccttaatg aaagcttata ttgctgtggt atagcatgtt catcagaagg
    15601 tcacaaaaca gaagctggaa actatcaaat agatttactt cttgcaaatg tgtgtaaaca
    15661 aaatgtaact agatttcctt atgaaatagt aagacttgca gaagatatag ctggagggct
    15721 tatggttact atgccttcag aaaaagactt taaatcagac cttaaagttg gaacaagtgg
    15781 aatgactatt ggcgaagtat gtaacaagta ttttaaagct tcttcagtgg cttcaacaga
    15841 agaaagaatg agaatattaa gattcttaga aaatatatgc cttggttcat ctgctgttgg
    15901 ttatagaact gagtcaatgc atggggcagg ttcaccacaa gctcaaagaa taatgatttc
    15961 aagacaagga aacataaatc aaaagaaaga attagctaag aaaattgcgg gaataaaaaa
    16021 agaggaagct ttaaatttat aaaaagttga tatataaagg gaaattatat attaatgtgt
    16081 gttaattaat gaaaaaatat gtagtataag agatagttat aaaattatgg tggtttttag
    16141 ctaagtaaag aaatttttaa taaggagaat ttacacatga cctgtggagt tagattttgt
    16201 gggggctgta accctagata cgaaagaggg aaggctctta caataatcaa agagcacttc
    16261 aatggtaggg tagattttag tatagcacaa gagaacgtcg agtatgattt gttacttata
    16321 attggaggat gcgcaactct atgcgcatcc tatcaacaat ataaagtgaa aaatgaatgt
    16381 gttaagatgt gggattttag ccatatagaa agagttataa atgtgattga atcagcaatt
    16441 tgtgaaactt aaaaaagcta aatgggggga tttcattgag ttggcaagaa ttgtatcaaa
    16501 gtaaattatg tagtgctaca gaagctgtaa aacagattaa aaacggtgat actgtagtat
    16561 ttgcacattg tgtaggtgaa cctcctgcac tagtagaagc tatgattgaa aatgctgaac
    16621 aatataaaga tgttgagatt aaacatatgg taagcttggg tagtggtgga tatactgcta
    16681 agggaatgga agctcatttt agagtaaatc caatgtttgt aagtggaaat gtaagaaagg
    16741 caattgaaaa tggagatggt gattttacac ctgcattctt ccatgaagta ccaaagttgc
    16801 ttagagaaaa aagattaaaa tgtgatgttg ttttagcaca ggtaactcca ccagatgaac
    16861 atgggtattg tagcttagga acatcagttg attatactta tgaagccatt aaaactgcta
    16921 gaacagtaat tgttcaagtg aatgaccagt ttcctagaac ctatggtgag gtagtccatg
    16981 tatcagagtt tgactatata gtggaaaaat cacaaccttt atttgaacta caacctgcaa
    17041 agattggaga agtagaagaa gcaattggta aaaattgtgc ttcattaatt gaagatggct
    17101 ctactttaca gctagggatt ggtgggatac cagatgcagt tatgttattt ttaactgata
    17161 aaaaggattt agggattcat tcagaaatga tttcagatgg gactttggcc ctttatgaaa
    17221 aaggtgttat aaatggtaaa tataaaaatt ttgataaaga aaaaatgaca gttaccttct
    17281 taatgggtac taaaaaatta tatgactttg caaataataa ccctgcagta gaggtaaaac
    17341 cagtagacta tgtgaatcat ccagcaatta taatgaaaca acataagatg gtttctataa
    17401 attctgctat acaagttgat ttaatgggac aggtagttgc agaggctatg ggacttagac
    17461 aatttagtgg tgttggaggt caagttgact ttatacgtgg agtgtctatg ggtgaagatg
    17521 gaaaggcaat tatagctatg ccttcaataa ctacaaaaaa agatggtaca gtaatttcaa
    17581 aaatcgtatc tattgtggat gaaggtgcac ctataacgac atcaagaaat gatgttgact
    17641 atattgtaac agaatacggt atagcagaat taaaaggaaa atctcttaga gaaagagcaa
    17701 gaaatttaat aaatatagct catccaagtg taagagaaca tctagcagta gaatttgaaa
    17761 agagatttaa ggagaaatat taataataga ggggtgttaa taatggaagc acttagagta
    17821 gttccaaaga tattttactt tgatacattt aaagaattta atgaagaatt taaaatagga
    17881 aaaaatgatt tagtaattac taatgagttt atatatgagc catatatgaa acctcttgga
    17941 atagatacaa atttaatttt tcaagaaaag tttggaacag gtgaaccatc tgatgaaatg
    18001 atagactcca tgacaaagga aatgaagaaa tacaattttg atagaattat tgcatttgga
    18061 ggaggaacaa tagttgatat atgtaagata ttagcacttg atgttccaga aaagtctatt
    18121 gatttatttg aaggagaagt atcacctaaa aaagtaaaag agttagtagt agttcctaca
    18181 acatgtggta caggtagtga agtaacaaat gtagctattg cagagttaaa atctaaacat
    18241 actaaaaagg ggcttgcagt agaagaaaca tatgcagact atgcagtgtt aattccagag
    18301 acagtaaaag ggttacctta caaattcttt gttacaagtt cggtagatgc tcttatacat
    18361 gctattgagt cttatctttc tccaaaagca agtccattta cagagatgta ttctctacaa
    18421 gcaataaaaa tgattatgga tgggtataag aagatagttg ataaaggaga agaagagaga
    18481 tttaatcatc ttagagactt tgtacttgca tcaaattatg caggaattgc ttttggaaat
    18541 gctggttgtg cagcagttca tgcactatct tattcaatag gtggagcttt ccatgttgca
    18601 catggtgaag caaattatca gttctttact gaagtattta agatgtattc tagaaaaaaa
    18661 cctaatggaa aaattataaa atgtacaaaa attttagcag atgcccttga atgtgaccct
    18721 aactgtgatg tttatggaga attagaaaag tttttaaata aattaatagc taaaaaagct
    18781 ttgagagaat atggaatggt ggaaagtcag atagatgaat ttacagatag tacaatagca
    18841 aatcaacaac gactacttgc taataattat gtagagctta gtagagagga aataagagag
    18901 atatttgcaa atctttatta atacaatttt ttatttatat gtatagtcaa cataaacaat
    18961 gctaattata tttatgtaca aataaaaata agggaactta aattttaatt aaagttaagt
    19021 tctcttattt ttatatggag ttaaatataa gtatattttt aatttaaaat atgcttatat
    19081 ttaagtctat tactttattt gaaacaattt gtagaatttc aaattccttg acagtaataa
    19141 gtaagtaatt gtataatcaa actgcaatat attaataata ttagtgaaaa tgaaaggatt
    19201 ataaaatatg tataaatcag aaaatttttc aaaaaaatct gtattaataa attcattttt
    19261 aagtaaattt ttaaaaataa ttggatgggg tttggtaagc ttatttacat tatcagcctt
    19321 agtactttca actatttcca ttataattgg atgggctgta atacttccta ttcaagtatg
    19381 tgcagtattt ttacttatag gaggagtttg tatttttaaa ggtaagagat taggagacca
    19441 aatttctcgt tataaaaaat attgtacaat tataaacaat agaaatatta ttccagtaga
    19501 acttattgca gaaaacacat ctaaatcatt aaattttata ataaaagatg tacaaaagat
    19561 gatagacaaa aactacttta taaatactta tattgataaa agaaataatc agattgtgtt
    19621 aacaaatgaa gattttgttc caccaagata tgaagatgta gcatatgaag tcaaagaaga
    19681 agaaaagact gatgaatcca atgaagttga tacaattata aataaaggaa tgaattatct
    19741 aacacaaata aaagaagcta ataaaaatat taaatcagaa tcaatgggcg ataaaattgt
    19801 acaagtagaa gatgttacaa gtaaaatatt tgatgttgta aaacatgacc catcaaaact
    19861 tactcagata caaaaattta tggattatta tcttccaacc actttaaaac tattaaattc
    19921 atatcataca ttggaagaac aaggaattga tagagaaaat ataacaacaa ctatggaaag
    19981 tattgaaaat acaatgaata cgattgtagt agcttttgaa aatcaattgg actatctgtt
    20041 tgaagatgaa gccattgata tttctacaga tattactgtt ttagagaata tgttagtcca
    20101 agaaggatta acgagtggta aattttaact tcaatagaaa aaatattctg ttatttttaa
    20161 acaattaatt agtcataaag tataaaatgt gtattatata agttaatcag tactttatga
    20221 aggaagagga gagaaagata caatgaataa tttagatgat ataccagtta tgccaacttt
    20281 gacacttgac ccatttggag aagaatcaaa cactaatgat atagataatt ctgacttatt
    20341 aatgaaaaag gatgaaaaag acccagaaga agaaaaactg tctgaatctg aacgcaagat
    20401 ggtaaaggaa tttgcggaaa aaatagatat tacaaataca aatatgattt tgcaatatgg
    20461 agctggagct caaaaaaagg tagcaagttt ttctgaaaca gctttaaaga gtgtaaaaac
    20521 aagagattta ggagaagttg gagatatgct tacaaatcta gttactgatt taaaagcatt
    20581 ttcagcagat gaaaaagaac aaagtggttt tttaggaatt tttaaaaagg caaataataa
    20641 aatttcaaat ttaaaaacta agtatgatag tgcagaggta aatgtggata aagtaagcaa
    20701 agagcttcaa aagcatcagg ttaaaatatt gaaagatata gcaatgcttg ataagatgta
    20761 tgaattgaac ttagcttact caaaagagct tactatgtat attatagcag gaaaacaaaa
    20821 attaaaggat atgaaagaat atgaaatgcc aaagttgaga gaaaaagctc gtttatctgg
    20881 ttcaacagaa gatgctcaat ctgtaaatga tatggtttca ctttgtgaca gatttgaaaa
    20941 gaagattcat gacttagaat taactagaat ggtatcgctt caaatggctc ctcagataag
    21001 attggttcaa aatagtaata atctgatggc agaaaagatt caatcaacta tagtaaatac
    21061 cattccatta tggaaaaatc aaatagtgct tgctcttgga atttctcact taaatcaagc
    21121 tatgaaagca cagcgtgaag ttagtaatat gacaaatgag cttttattaa agaatgcaga
    21181 aactttgaag atgggaacaa tagaaacagc aaaagaatca gaaagaggaa ttgtagatat
    21241 agacacaatt aagaaaacta atcaatctct aatatctact attgatgagg tagttaaaat
    21301 acaacatgat ggtagaataa agagacaaga agcagaagtt gaattatcaa aaatagaaaa
    21361 tgaacttaaa agtaagcttc ttgaatttac agttaaataa aaattatact aatataaaaa
    21421 gagactgtct caaaatagag ataagtctct ttttcattgt ttaaatgatt aaagtatatt
    21481 tatattataa atttctatta tcttttaaca taacatcatg agctccacct tcaactatac
    21541 tagtagaaga tacaagtgta agtttagcat ttttttgaag ttcaggaatg ctaagtgctc
    21601 cacagttaca catagtagaa cgaactttac ttaaagaaag agttacattg tctttcaaag
    21661 aaccagcata aggcacataa gagtcaacac cttcttcaaa agaaagtttc ttatctccac
    21721 caaggtcata tctttgccag tttctagcac gagcagaacc ttctccccaa tactctttca
    21781 tataactacc atttatatta actttgttag ttggactctc atcaaaacga gagaagtatc
    21841 taccaagcat tataaaatct gcacccatag ctaaagctaa agttatatga tggtcataaa
    21901 cgataccacc atcagagcaa attggaatat aaatacctgt tttttcaaag tattcgtctc
    21961 tagcttgagc aacttcaatt atagaagtag cttgtccacg tcctatacct ttttgttcac
    22021 gagttataca tatagaacca ccaccaatac caactttaac gaagtcagca ccagcttcag
    22081 ctaggtaaag gaatccttcg cggtcaacta catttccagc tcctacctta actgtatcac
    22141 catatttttc acgaatgaaa tcaagtgtta ttttttgcca ctcagaaaaa ccttctgaag
    22201 aatctataca taaaacatca gcaccagctt caactaaagc aggaacacgt tcagcaaagt
    22261 ctctagtatt aataccagct ccaactacat atctcttaga agaatctaaa agttcaagtg
    22321 gattttcttt atgagaagag taatctttac ggaataccat atacataaga ttaccattat
    22381 catcaagtac aggaagagaa ttaagtttgt ggtcccatat aatattgttt gcttccttta
    22441 aagtaacatc tttgttagca taaactatag atgacatagg agtcataaat tcactaactt
    22501 tagtatctaa atccatacga cttatacgat aatcacgact tgcaacaata cccaataatt
    22561 taccagttga agttccatct tcagtaacag ctactgtaga atgtccagtt ttttctttta
    22621 agtcaagtat gtcttttaat gtgttatcag gtttaatatt tgaatcactt acaacaaaac
    22681 cagctttgtg ggatttaaca cgtgctacca tagcagcttc attttcaata gtttgagaac
    22741 cgtatataaa tgaaatacca ccttctttag caagtgcaat agccattttg tcatcagaaa
    22801 cagattgcat aatagcagaa gtcaaaggta tattcatata aatatctgct tcctcgccct
    22861 ttttaaattt agtaacaggt gtttttaaac ttacattagc aggtatacaa tcagtagaag
    22921 agaaacctgg tacaagaagg tattcactga aagtatgtga tggtgtatca aaataaaacg
    22981 ccatttttta atcactcctt atatttttat actttttata attgatagta ttaattataa
    23041 cttattcttt aattttatta aagtattttg tcaaatattg agatttttga aaaatttaaa
    23101 ctattttaat taaatttgtt taatatatat tgccttatag ggtaaagttg tatattaaat
    23161 aatgtttagt gtttaatagt agctaattta aaattaatct ttagtgattc atataattac
    23221 atttgtatag tttacattga taatattact agagagtgca taaacctcaa aaatacagat
    23281 aaattaaatc tatattagta tatccaaaag tacatttaat aaatttattt aactgaatta
    23341 ttattttcat ttctattaaa tttaaatatt ttataaaaat agtattattt acaaaatcat
    23401 atactccaaa atactacata aataaaatta aacaaaaact aaaatgacaa atgtcattat
    23461 ctaaagtgac atagaaacgg tttcttaaag ttgttactat aaaatataaa ctctataata
    23521 aaagtataac aaaagggaat catatcgata tagaatataa ttcaagagga ggtaaaatta
    23581 tgagtagttc taatcaatcg acaataaaga catctataca gcgagtaggg aaatttttaa
    23641 gtggtatggt aatgccaaat ataggagctt ttatagcatg gggtcttata acagcattat
    23701 ttataccaac aggatggatg cctaatgaaa aattgagtac tataggtgac ccaatgataa
    23761 aatacctttt accactattg atagcttata ctggtggtaa agcaatagca ggacaaagag
    23821 gtggcgtaat aggtgcagca gcagctatgg gagtaattgt tggtgcagat ataccaatgt
    23881 ttattggagc aatgataatg ggaccatttg caggatgggt aattaagaag tttgataaat
    23941 ttgtagatgg aaagatacca acaggatttg aaatgttagt aaataacttt tcaataggta
    24001 taataggtat gcttcttgca atactaggat tttatgcaat tggaccagca atagtagcag
    24061 ggacagctct tatagaatct ggagttcaat ttatagttag caaaagttta ttgcctctag
    24121 tttcagtatt tatagaacct ggaaaagtat tattcttaaa caatgcaata aatcatggta
    24181 tattagggcc tataggtata gctgaggcaa aagaagcagg taaatctata atgttcttac
    24241 tagaaagtaa ccctggacca ggtttaggag ttctattagc atattggatg tttagtagag
    24301 gaagtgtaaa acaatcagca ccaggagctg taataataca tttctttggt ggtatccatg
    24361 aaatttactt cccatatatt ttaatgaatc cagttttaat attggctaca atggcaggtg
    24421 gagcagcagg aatattgact ttttcaatat taggagctgg gcttgtagca gcaccatcac
    24481 caggaagtat atttgcactt atggcactag ctccaaaggg aggattactt ccagtattag
    24541 caggagttgc agtggcaaca gttgtatcat tcttagtggc agcaccattt gttaagagag
    24601 cttcagcaaa tcaaagtgaa gaagacagta cttctttaga agaagctaaa gcaaaaatga
    24661 gtgatatgaa atctgcaagt aagaactctg agaaaaatat tgaggaaaaa cagcttgaag
    24721 taaatgagat taagaagatt gtatttgcat gtgatgcagg aatgggttcg agtgcaatgg
    24781 gagcttccag atttaaaaat agaataaaaa acttagactt aaatatagaa attacaaatt
    24841 catctgttga taatcttcca gatgatacac aaatagtagt aactcacaat acattagttg
    24901 aaagagttgc taagaataat tcaagtgttg agatagtttc aataaacaat tttttaaatg
    24961 acccaaattt agatacattg tttaaaagat tagaatctaa ataaaaataa aaagtaccac
    25021 taattaggag tggataattt gaaaaaaaag aagattacat ctaggcaaaa aaagataata
    25081 ctaatgatag tagcaaattc aaagaaaaac attccaataa ctatttctga gattgcaggg
    25141 actttagagt taagttcaag aactgtactt agagatatgt ctggaattga aaaatggttt
    25201 gatgaaaatg attttaactt tgtaaaaaaa ccaggagtgg gattgatttt agaggaaaat
    25261 atagaaaatc aaaactttat aattgagttg ttagaagaag aaaaaataga aaaagaatac
    25321 agcaaggaag aaagaaattt aattatatta tcaaagctac tagtttctaa cgagccagta
    25381 aaatcctatt attttgcaaa aatattaaag gtatctgaat gggtactgaa taatgatttt
    25441 gccttagcaa gtaaatggtt ggaaagattt gatattgagc ttgttagaaa accagggctc
    25501 ggtgtctact taaaaggtca agaaaaaaac tttagagagg catatgtaaa tcttatatac
    25561 gattcattca atgaaaaaga aatactagat atggtcagaa atataagtga aaatatacaa
    25621 acagataaag caatagaaat tttaagtgaa aatagacttt taaatttaat ggacaggtgc
    25681 ataataagaa aagttgaagc tacattaaca aagaagcttt cagatttaga tgtaaattta
    25741 gctgatagtg catatatagg gcttgtagtt catatttcat tagccttaca gaggataaaa
    25801 aatggtgaaa atataactat ggataaagag tttctaaaag aattatctat aacagaagag
    25861 tttaaattag ctaaagaaat cgtaaaaggt atggaaacag attttaacat ggatatacca
    25921 gtagatgagg ttggatatat aactatgcat ataagaggtg caaagcaaag gtcatcttca
    25981 aaccataagg ctttaaactt agatgatata gaaataatgg aaattacaaa taagatgata
    26041 gatttggcgg aagatgaatt taaaataagt ttaaaaaatg acgagaggtt atttaaagac
    26101 ctagcaaatc acttagggcc ttctataaat aggttgaata tgggattaga aataagaaat
    26161 cctcttttag atgaaataaa aagcaagtat tcatatgcct atgatgtagt tgaaaagatt
    26221 tctagaatta taaaagataa actaaatata aattcaatac ctgaatcaga aataggatat
    26281 attgcaatgc actttgcatc agccatagaa aaaaatctta tgatgaatac taatataaat
    26341 attgttgtgg catgtcccac tggaataggt acttcaagat tcttatcaac taagattgaa
    26401 aataaatttc ctaatttaaa tatacttgaa accatatcag ctataaacat agatgaagag
    26461 tatttaaagg aaaaagatgt tgatttaata gtgtctacag ttgagttaaa tacaagttta
    26521 aattatatat gtgtaggtcc atttatgagt ttagatgatg aacagataat caaggaaaaa
    26581 ataaaatcta tagctcaaaa taaattaatc aatttaaatg cgaaaaatga tactaagagt
    26641 aagaataaag tatatgaaca gataacagaa agtatgaata taggaaaaga tattcttcaa
    26701 tttttagagg aaattagatt tgaaaaattt gaaagtaaag atttaagtga gcttattgaa
    26761 gactcatcaa aaatatttgc aaaatctagt gaagatataa tctctataaa agaatcgtta
    26821 aaagaaagat taaaaatttc aattccatat atagaagaat caaaaattct attgttacat
    26881 tgtatgagtg aaagaataga tattatgaag ttatcaataa taaaactaga gaatagaatc
    26941 gtgctagatt caaatgaaga aatcgacaat gtagttttta tgttacttcc taaaaattca
    27001 ccaagttatc aaagacaaat aatgagtgaa ataagtggtt cattaataga caatataatt
    27061 tttactaata agataaataa attctctata gaagagatgg cattagaggt aaaagatata
    27121 gtttttaatt tttatacaaa cagattaaaa gcatttatag ataaataaaa ttaatattaa
    27181 aaaggaaggt tgatactatg agtaaagtat tgaatgagaa taatatattt ttagggttag
    27241 atagtgtttc aaaggaagaa gctataactt tagcaggaag aaaattagtt gaaaatggat
    27301 atgtaaaaga agaatatata ccagcaatgt tagaaagaga aaaagtaatg actacatata
    27361 tgggaatggg agttgctatt ccacatggtg taaatgaagc aaaaaaagaa atattatctt
    27421 ctggaatagt tatattacaa ttccctaatg gtatagattt tgatggagaa aaagcatatt
    27481 tattaatagg aatagctggt gttggagatg aacatttaga aatactatct aatatagcag
    27541 tagttttaga tgatgactta acagaaagac taaaaaattc aaatgacaaa caagctttta
    27601 tggaagcttt tgccaactaa tcttaggagg gatttaaaat gaaaaaggca attcagtttg
    27661 gagcaggaaa tattggaaga ggttttattg gaggcttgtt agttaagtcg ggttatcatg
    27721 tagtatttgc tgatgtaaat gaggagatat taaactcaat aaacaaagat aaaaagtata
    27781 ctatacacat aagagatgta gaatgtatag atgaagtaat tgataatata agtgcggttt
    27841 catctataaa agaagaaata atagatgaga ttgtacaggc tgaaataata actacagcag
    27901 ttggaccttt agttttgact aaaatagcct ctacaattgc aaaaggtata aaggctagaa
    27961 aagaaaaggg attaacaagt aatctaaata taatagcatg tgaaaatgca atatacgcaa
    28021 gttcttcttt aaaagaagag gtacttaagt atttaaataa ggaagaagta gaatatttag
    28081 agatgtatgt tggttttcca aactgttcag ttgatagaat agttcctcct ggtaaaaacg
    28141 aaaatcctct tgatgtaaca gttgaaaatt tttatgagtg gaatgttgaa aaacaaggat
    28201 ttaaagggga aataccaact atagttggta tgaatttagc tgataatctt atggcttata
    28261 tagaacgtaa attgtttaca ctgaatacag gtcatgctat aacagcttat ataggatatt
    28321 taaaaggata taagactata gaagagagta taaaagataa atttatctgt gatatagtca
    28381 aaagtgcaat ggtagaaagt ggagaaggat tgattaaaaa atacaatttt gactcagaag
    28441 tgcactataa atatattgat aaaattttaa atagatttaa aaatccatat cttaatgatg
    28501 atgtattaag agtcggaagg gaaccattaa gaaaactaag tgataaagat agattgatta
    28561 aacctttaat gacagcaaaa tcatatggat tatcagttga taatttgatt cttggaatag
    28621 gtgcagcgct tcattataat aatagtgaag atactcaaag tgtagaatta caagagttga
    28681 taaagtctat tggagtaaaa aaagccgttg ctaaaatagc taatataagc aatgacgaag
    28741 aacttttaaa caatatcgaa aaaagttata tatttatgaa aaatttataa atttacttga
    28801 aaaaaagtag ttctgataaa gtaactataa tatattattc tacacagttt gaattatttg
    28861 ttg
//
LOCUS       DAYAOX010000025        28649 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.025, whole genome shotgun sequence.
ACCESSION   DAYAOX010000025 DAYAOX010000000
VERSION     DAYAOX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.025"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            142..492
                     /locus_tag="ACGI57_000912"
     CDS             142..492
                     /locus_tag="ACGI57_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFG9715998.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            511..2154
                     /locus_tag="ACGI57_000913"
     CDS             511..2154
                     /locus_tag="ACGI57_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFG9715999.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2235..4292
                     /gene="recG"
                     /locus_tag="ACGI57_000914"
     CDS             2235..4292
                     /gene="recG"
                     /locus_tag="ACGI57_000914"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFG9716000.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4351..4905
                     /gene="rsmD"
                     /locus_tag="ACGI57_000915"
     CDS             4351..4905
                     /gene="rsmD"
                     /locus_tag="ACGI57_000915"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFG9716001.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4907..5404
                     /gene="coaD"
                     /locus_tag="ACGI57_000916"
     CDS             4907..5404
                     /gene="coaD"
                     /locus_tag="ACGI57_000916"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFG9716002.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5408..5932
                     /locus_tag="ACGI57_000917"
     CDS             5408..5932
                     /locus_tag="ACGI57_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716003.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6276..8195
                     /locus_tag="ACGI57_000918"
     CDS             6276..8195
                     /locus_tag="ACGI57_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9716004.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENIL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8208..8495
                     /locus_tag="ACGI57_000919"
     CDS             8208..8495
                     /locus_tag="ACGI57_000919"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9716005.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8628..9887
                     /locus_tag="ACGI57_000920"
     CDS             8628..9887
                     /locus_tag="ACGI57_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFG9716006.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9890..10630
                     /locus_tag="ACGI57_000921"
     CDS             9890..10630
                     /locus_tag="ACGI57_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9716007.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10953..12263
                     /locus_tag="ACGI57_000922"
     CDS             10953..12263
                     /locus_tag="ACGI57_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFG9716008.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLISS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12361..13278
                     /locus_tag="ACGI57_000923"
     CDS             12361..13278
                     /locus_tag="ACGI57_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFG9716009.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            13294..14103
                     /locus_tag="ACGI57_000924"
     CDS             13294..14103
                     /locus_tag="ACGI57_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFG9716010.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14161..15909
                     /locus_tag="ACGI57_000925"
     CDS             14161..15909
                     /locus_tag="ACGI57_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HFG9716011.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKVREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     RYDKVKKYVLELLDRV"
     gene            16213..17193
                     /locus_tag="ACGI57_000926"
     CDS             16213..17193
                     /locus_tag="ACGI57_000926"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HFG9716012.1"
                     /translation="MFFLSISFIGFYLVIRDNSDTVKMQRGINIGNALESPKDFPWDV
                     KMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKIDKYVDYALDKDLIV
                     VLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELLNEPKENLSSQLLNE
                     YIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVVSFHYYEPNDFAFQG
                     NIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIFLGEFGITKEAPEVS
                     RRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILSALIEKR"
     gene            17198..19504
                     /locus_tag="ACGI57_000927"
     CDS             17198..19504
                     /locus_tag="ACGI57_000927"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9716013.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTTS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            19515..20591
                     /locus_tag="ACGI57_000928"
     CDS             19515..20591
                     /locus_tag="ACGI57_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716014.1"
                     /translation="MKTLKVFFCSTKYLILVFIFFINTTFISSATSDKILDLSFKKIE
                     TDLNSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNFYIEIQNKNKSSQR
                     INLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEFEGKIYVNFNSLIN
                     EESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKLRFLNNIKIIGDEE
                     VQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTLSNKAKPGQIILQV
                     NVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMKKINFMNNIITFVR
                     ILFVSLVIICFGIYLYWKKCSKTK"
     gene            20609..22027
                     /locus_tag="ACGI57_000929"
     CDS             20609..22027
                     /locus_tag="ACGI57_000929"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HFG9716015.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22042..23250
                     /locus_tag="ACGI57_000930"
     CDS             22042..23250
                     /locus_tag="ACGI57_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716016.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(23324..24712)
                     /locus_tag="ACGI57_000931"
     CDS             complement(23324..24712)
                     /locus_tag="ACGI57_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFG9716017.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            25053..26387
                     /locus_tag="ACGI57_000932"
     CDS             25053..26387
                     /locus_tag="ACGI57_000932"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HFG9716018.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26529..27446)
                     /locus_tag="ACGI57_000933"
     CDS             complement(26529..27446)
                     /locus_tag="ACGI57_000933"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFG9716019.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27654..28538
                     /locus_tag="ACGI57_000934"
     CDS             27654..28538
                     /locus_tag="ACGI57_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HFG9716020.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
BASE COUNT        11196 a         3063 c         5094 g         9296 t
ORIGIN      
        1 ttatccaaaa acttttctag gtttaaatta taccatattt atagtatgat atgattataa
       61 ttttatataa taaataagta aaaatagtaa aaaataaatt aagaaatgtt tttaataaaa
      121 ataagcttaa gggggtcaac catgagtgca aaggtaacga atcaatatgg aaatatagaa
      181 atagataatc aagtaatagc tcaagtcgta tatagagctg caatggaaag ttatggctta
      241 gtaggtttag catataagtc taaaggaata gtagagctat taaaaggcga gaacgcaact
      301 aaaggtgtaa gagttgagga agtagaagaa gatgccatag caatcgaact ttatgttata
      361 atacaatatg gtacaaatat atctactgtt gccaataata taatagatag agtaaaatat
      421 gtagttgaaa aaatgactgg cgtaagagtt actagaattg atattaatgt acaaggaata
      481 agagttaagt aatttcatag gaggaaacaa atgattcaat atatagatgg taaaaggtta
      541 agggagatgt ttatttcagg tgcaaacaat ctccaaaaca ataaagagtt agttgacaag
      601 ttaaatgtat tccctgtacc agatggagat acaggaacta atatgtcatt aacaatatct
      661 tatgccttaa aagaattagc aaaagttgaa aatgataata tatcagatat cggaaaagca
      721 ttatctaaag gttctttaat gggagctaga ggaaactcag gagttatact ttcacaaata
      781 atcagaggta tagctaaatc tatagaagga aaaagtaaat tatcaacaga agatttagca
      841 aaagctttta aaaatggttc agacacagca tataaagctg taataaaacc aattgaagga
      901 actatactta ctgtagttag agaaagtgga gagtttgcaa taaaaactgc gaaaaaagag
      961 aaagatgtag taaagttttt aagtatgctt gtgaaggaat caaattcttc attagaaaga
     1021 actccagacc ttctaaagaa tctaaaagaa gctggtgtgg ttgactctgg tggaaagggt
     1081 cttgtactta tatatgaagg aatgttagct tctataaaag gcgataacat agaaataaaa
     1141 aatgttgacc tagatactaa tatttcaaca agtatggatt ttgcaaagag tactactagc
     1201 acagatgata taaaatattg ctattgtaca gaatttattt tagaaagttc taaggtagaa
     1261 gatactaaaa taagagacat aatgatggct tatggtgata gcctagcagt agttggagat
     1321 gatggagtca taaaggtaca tgtacacact aatgacccag gaaatgtact tcaagaagct
     1381 ctaaagtacg gtcaattatt aactataaaa atagaaaata tgaagttaca acatgaaaat
     1441 acattactgg atgtagaaga aaagaaagaa aatgatagtg aacctttaga agaagaaaaa
     1501 gagtttgggt ttatagctac ttctatgggt gaaggtttgg caaatatatt taaagatttc
     1561 ggagttgacc atataataga aggtggtcaa actatgaatc caagtactga agattttatg
     1621 aatgctataa agcatataaa tgctaaaaat atatttattt tcccaaataa cagcaatata
     1681 ataatggctg ctaatcaggc aaaagaattg agtgataaaa atataatagt tatacctaca
     1741 aaaaatacgc ctcaaggatt tgctgcatta gtaactttta atggagaatt aagcgaagat
     1801 gaaaataaag aagctatgat gaatgcttta aactcagtta aatcaggtca agtaactttt
     1861 gctgttaggg atacagtgat gaatgaaatt gatgttaaag aaggtaatat aattggaatt
     1921 gcagaaggaa atttattatc agcaggagat tatgtggatg aagtaacatc aaaccttatt
     1981 gaaaagttgg ttgatgaaga tactgctata ataacattat tctttggtga agatgttaca
     2041 gaatctcaag caaatgaact acgtacttct ttagaagaaa agtttgaaga tgtagatgta
     2101 gagttatatt atggaggaca acctctttat tattacttaa tttcagttga ataatgatta
     2161 taagaacctg tatgaccaag tagcttttat aaaattggtt gtacaggatt tttgtgtagt
     2221 aggtgatata tttgttggat ttatataaag atgttcaata tgttaaggga ataggaccta
     2281 aaaaagcaga taaattaaat aaactgggta tatttacact aaaagaccta ttgtattatt
     2341 ttccaagaca atttgaagat agaaataatc taaagaaaat agcacaatta gaagatggtg
     2401 agaaagttac gataaaagca gtaatatcaa gtataaatac atttagccca aaagaaggta
     2461 tgactttaac aaagattgat gtaaaagatg agacaggctc ggctaaatta gtgtttttta
     2521 ataagagtta tataaaaaat acttttcgtc caggagattc aatattagtt tttgggaagg
     2581 ttaagaaaaa atttaataac ttagaactta catcatgtga attagaatat cttactaatt
     2641 cacctaagaa tacatgtaga tttatgcctg tgtaccagct tacatacggt gttacaaata
     2701 aagaaattat gagtataatt agaacagtac ttgaagataa agaattaatt atacaagaat
     2761 atatgccaca aagaattata gaaaaatata ggctatgtag tatagatttt gcagttagaa
     2821 acatacattc accaagtagt aaggaatcat taaaaatagc tttatataga attgtatttg
     2881 aagaattact tatactacaa ctagggttgt ttgtatttaa aagtggcaga aataaagaag
     2941 atggaataaa gtttgaaaca agtaaagact taaaaaaaat aataagtgct ttaccattta
     3001 aactgactaa agctcaaaac agggcattag atgaaattat acaagatatg aatttagaga
     3061 aaataatgaa tagattggta caaggtgatg tagggagtgg taaaacagta gtagctctct
     3121 tagcacttgc aaattgtgtt ttaaatggtt atcagggcgc tttgatggca cctacagaga
     3181 tattagcagg gcaacattat atttcactta ctgagtctct aaaagatttt ggaataaatg
     3241 taggtctatt aataggtagt cttacaaaaa aacaaaagga tacagtattg gaacaaataa
     3301 agaataatga gattgatata ttaataggta cacatgcact aattgaagat aaggttgaat
     3361 ttaataatat tggtttagtt ataacagatg aacaacatag atttggtgtt atgcaaagga
     3421 gcaaactttc attgaaagga gcaaatcctg atatattagt aatgacagca acaccaatac
     3481 caagaacttt agcacttata ctctatggag atttagatat ttctattata gatgaacttc
     3541 ctccaggaag acagccaata gaaacaattg ctattgaaaa aagtaagcga gatagagcat
     3601 ataataatct tgtaagaaga gaggttgaat caggaagaca agtgtacatt gtatgtccct
     3661 tggtagaaga aagtgaagcc atagaagcca aatcagcagt tgaacttgtt gaggagttaa
     3721 gagcggaata ttttcacgac ttaaggcttg ggttgcttca tggtaaaatg aagtcaagtg
     3781 aaaaagatga agtaatgaga ctttttaaag ataaagaaat agatatttta gtttcaacaa
     3841 cagtaataga agttggtgtc aatgtgccaa atgcaacatt aatgataata gaaaatgcag
     3901 aaagatttgg acttgcacaa ttacatcagc ttagagggcg agttggaaga ggtagtcata
     3961 aatcgtattg tgtactaatt tatgattcta aaacagatgt atgtagacaa agaatggcta
     4021 taatggaaga gaccaatgat ggttttaaga tttcagagaa ggatttagaa ataagaggac
     4081 ctggggaatt ttttgggaca aggcagcatg gtcttccaga acttaaggta gcaaacttat
     4141 ttaagcatat aaaaatactg aagttagcac agcaagaagc tcgatatata ttgggtgaag
     4201 ataataatct acaattaaaa gaaaacatgg cactcaaaaa agaaattata gataaattta
     4261 aagatacatt gaaagaaatt tcattaaatt aattttaaat tgtgttaaaa tattactaat
     4321 gtaataatgt gctgaaggga gattgtcata ttgagagtaa tttcaggaaa agcgagagga
     4381 ttaaaactaa atactccaaa aaatgaagat gtaaggccaa ctacagatag agtaaaagag
     4441 tctctattta atatgataaa ttcatatata atggaaagtg aagttctgga tttatttgct
     4501 gggactggtt ctttaggaat agagtgctta tctaggggtg ccaagtcatg tacttttgta
     4561 gatattagta aggaaagcat agatattgtt aagtccaata taaaaaaggc aagagttgaa
     4621 agtgaaagta ttattttaaa ccttgatttt aagactgcaa tagataaact taaattacaa
     4681 aatagtaaat ttgacataat ttttatggac cctccgtatt ataaaaatat gtttatagaa
     4741 gctattgaaa aaattgataa ttctaatcta ttgaacgaag atggaataat agttgtagaa
     4801 catgatacga atgatttatt tccagataag atatgtaaat tggagaaaac tagagataag
     4861 aaatatggaa atactacatt aactttttat aagatggagg cataatatgg aaaataaacc
     4921 tagaaaagct atatttgcag gaagttttga cccaattact aatggacatt tagatattat
     4981 ttgcagggct tcgaagttat ttgacgaatt acaaatagga gtattaaata atcctaataa
     5041 aaagggtcta tttagctttg atgaaagagt aaaacttata gaaaaaagta caagccattt
     5101 aaataatata aaagttgtga cttttgatgg tttattaata aattactgtc aggaaaatgg
     5161 gattggagca ttagttagag gtgtgagaag tggtgctgat gttgattatg aacttcagat
     5221 ggcacatatg aatagagagc ttaatccaga tatagagact ataattttac caagttgtac
     5281 aaaatattca tttataagtt catctttaat aaaagaggtt ttgctctttg atgctgatat
     5341 aaaaaatttg gttccaaaga ttgttttgga agaattaaag aaaaaaacta gtgggggtaa
     5401 ttgatatatg aagatagatt tagaaatgat tgaactattt gagcagttag aggagatatt
     5461 tagaagtgct tctacaatcc ctttctcaca taaatgcaca gtggataaag atgaggtttt
     5521 agcaataatt aatgatataa gaactttaat accagaagaa gtaacacagg cagtatggat
     5581 taataaagaa agaaataaaa taataagtca agctaagcaa gatgctacaa atatagttga
     5641 acaagcgaaa aaagaagctg agagaataca acaagaatat caagataata tagaggaatt
     5701 aaagaaaaat tctgaagatg tagtaaaggc atatgtagaa tccagtgaac ctgttgttca
     5761 agcagaccaa agagcaaaag atatagttga tagagctgag agaatagcca atgagataag
     5821 aattgggtca attgaatatg cagaggatgt gctttctagt gttgagtata atttaagaga
     5881 aatattagaa gaagtaaaaa gagataaagc tgaacttaga cctaaaaaat aaattttaaa
     5941 taaataaaaa tggagctatc acacaaaaaa ttccaattta tgtgtgatag ctctctttta
     6001 atatagcaat tttttgttat ataattgtgc tttttagatt acttatatct tattttgata
     6061 ttataatgta gtttaatctt ttatttgttg aaatcaactt atttatttgt ataaataata
     6121 taggttattt tcaaattttt ttaataattt taccactgtc ttcttttaga ttagagttag
     6181 actgtgcaaa attataaaat aatactaaat catgtgcaat tatttggtat ggcactacat
     6241 gtagaaaata tagataataa aattaaggag gtagtatgaa ggaaagggct aaaaaaatat
     6301 tagattttat tattaaaaat aacaatgtaa catcacaaga actacaagaa aagtttaatg
     6361 tttcaaagag aactatttat tacgatattc tagctataaa tgaacaacta ggaaaaagtg
     6421 gtaatataaa aaatgtaaaa cacaaattta tatttgaagg taatttatgt gatgcaagga
     6481 aaataatttc aactgaagaa gataaatttt tagatagtga ttacagaaaa acttttatat
     6541 taaacaagat attacttggc gaaaaaatat caatagaaaa actaacaaat gaaatgttac
     6601 tttcaaaaaa cacagtagta caaacaataa ctgatgtaaa aaaatacctt caaacaatgg
     6661 ggttaagatt agagtataag ggaaagtaca aaattatagg tgatgaatac gttattagag
     6721 agctgtttct gataattgtt caagaaaata tattggaaat taattctata agtgaggaag
     6781 taagtagttt cgatacaaaa ggacatataa aacttactga ttattcttta ttaaatctaa
     6841 ctaagtttgt ggaatttcta aataaaagga taagagatgg gaaaacatta tactcatata
     6901 aatatctaaa tgaagctaaa aaaattagtt actttagtaa ttgtaaagaa cttttatgtg
     6961 aagaagctaa tgaaaatgaa caagcctata tatgcactta cataagttca cttcccagct
     7021 taaatagtga agttaaggaa gatgttgttg aagaatatgt agataaactt atagataagt
     7081 ttgaagtaaa tacagcaata aaattagaaa gtaagcatga gtttaagaaa aatattttaa
     7141 ggcacctcca ttcttcttac aacagaatac gatttaagtt tcctattaga aatccaatgt
     7201 tagatgagac taagtataaa catgagtcac tttataaaat tataaaatca attatagaaa
     7261 atgaagaaga gtttcctgta tttgagggga taagagaaga agaaatagga tttatagcag
     7321 cgtattttgg aggatattta agagggtcta gggataatgg tctaagaaga aataaagtct
     7381 tactagtttg tcctaatggt cttatggtat caaagagttt ggaaatacaa ctatataaat
     7441 acataccaac catagaaata gtaggtatag tatccattaa gcaactaaaa gaagtaaatg
     7501 tatactacga ttacataatt acaactatag atatacaaaa tgtaaacaac gtaattgttg
     7561 taaatcctct tttaacatca tcagatgttc aattacttat gaacaaacta ataagtgtaa
     7621 aagaaaacga gaagtatttc aatcttgaat taataataca agctataaga aaaaatggag
     7681 ttatcaataa tgaagaagca ctaaaagctg atttattaaa cataatacac aagatagatg
     7741 aaggagagat gtatcaacca atgttaaagg aattaattaa tgcagaaagg gtcaatataa
     7801 taaaaagtgt tagagattgg aaagaagcaa taaaaatagc ctcaaagcca ttgttagaag
     7861 ataactctat agaagagtta tatatagaaa atatgataaa gtcagttgag aaatatggac
     7921 catatattgt tttagctgat agatttgctc taccacatgc aagttctaaa gaaggagtaa
     7981 acaagcttgc tatgtcttta ctaatagtag aagatgaagt tgatttatta ggaaaaccag
     8041 taaatatatt tatggtatta gctgctgtag ataacactac tcacattaga gcattagcat
     8101 cactatcaga gatgatgtac gaggaagaaa atgtaaaact aataattaat ggtgataaaa
     8161 gttcaatact agaacttata aataagcaaa attaggagga atgaaaaatg aagatattag
     8221 cagtatgtgg ttttggtgta ggttcatcaa tggtattaaa aatgacaata caaaaggctc
     8281 taaagcagtt aggtatagaa tgtgaagtag aaaatacaga tataaattca gctagaggtg
     8341 ctgattgtga ctgtatattc acaagtcaag aattgttaaa tgaattaaaa ggaacttgta
     8401 atgtagatgt atatccagtt aaaaagtaca tggatttagc agaagtcaca gaagttgttc
     8461 aaactttttt aagtgataaa aatgataaga aatagtataa gttttaaaaa gtaaatttaa
     8521 gttaatcaga ataaaataaa aacttactaa attcaaaaat agtaagaaag agaggaggga
     8581 tttagtggta gaaaacttaa acagttgacc tatcactaaa aaatataatg cttgatttta
     8641 tttcaaataa tattttacgt aatccatcta tgttaattgg attaatagca gcatttggtc
     8701 ttgtactaca gaagaagaaa gcatctgatg ttataaaggg gtcactactt gcagctttcg
     8761 gaattataat tatggagact ggaacaggta tgttagttag ttcaatagca ccaattaatg
     8821 gagctttcca aagtataagt ggtggtgcag taacaaaagg tttaagtgat gttactttta
     8881 ctgcaagtta tggtggaatt ataggtttag caatgtttgc gggtttagct attcacctaa
     8941 tgatagcaag atttactcct attaagacaa tctttttaac aggtcacatg ctttattggt
     9001 tcccattcat atttgtagct tcaggagttg aagctggatt aaatggtgga gtattaatag
     9061 cttttgcagc agtattatca gcactctatt ggtcaataat gccttggtta ttaagaaaat
     9121 atgtgtttga tgttatagga gatgattctt ttacattagg tcatccatct ggttgtttag
     9181 cacttatatc aggatttgtt gctaaattag taggaaataa agaaaaatct acagaagata
     9241 taaacatacc tcaaagttta tcatttttta gagaaatatc tataagtggt ggaatggcag
     9301 tgtttttaat gtttatagtt gttgggatag taataccagg tgcgtttaaa gatagtaatg
     9361 agtttattgg aacgtatgca ataaagcaag gtctttcttt tggagcaggt cttataataa
     9421 tgcttcatgg ggtaagaatg ctaataaatc aaataatgcc agcatttcaa ggtatatctg
     9481 aaaaagttgt gccaaatgca ctacctgctt ttgactgtcc aatattattt aattataaac
     9541 caaatgctgt attaatggga ttcttagttg ctatgctaac atctacagct atgattttaa
     9601 tatgtaacta ctttaatgta tttaatttgc tacttatacc acttgttata acttcattct
     9661 ttgagtgtgg agcagcagca gtaataggag aatcacaagg tggtatacgt ggagctataa
     9721 taggtacagc tatagctgca atatttatgg tagttttagt aggaatatct gctgtaattt
     9781 actcaggaac tattcaaaat tggatactaa tatttggagg taatgacctt tcattatttg
     9841 gtataatcag taggtttata ggttcactat ttggaggaat attatagata tgaaatttaa
     9901 atacatagat gagataagaa gattaattga tataatagaa aacgaagaaa aagtcaatat
     9961 ggaaaaagca gtacaaatct ttgttgaagc aatagaaagt aaaaatgcta tattttcatt
    10021 tggagcttca catgcaggaa tattaacaga agaattattc tatagagcag gtggctgtgt
    10081 tcttataaat ccagtatttg caaaatctct aatgttagat acagcgccta taactcacac
    10141 aagtaagatg gaaagattag aaggatatgg tacagctcta gctgataaaa ctccaattaa
    10201 aaaaggagat gtagttatag ttcattcagt atctggtaga aatccagtaa gtatagagtt
    10261 tgctatgaaa gctaaggaaa ataaggcaat agtaatatgt ataactaatt taagttactc
    10321 aaaaagtgta tcttcaagac attcttcagg aaaaaaactt tatgaagtat gcgatttagt
    10381 gatagataat catggtcaaa agggagatgc ctgtgtagag atagatggat taaaccaaaa
    10441 agttagtcca acttctactg taatagcaac tactgtgatg aactctatta tagctcaagc
    10501 tactcaggaa ttagttaata aagggctaaa gaatcctccg attttctata gtgcaaatat
    10561 tgatggtgga gatgagttaa ataagaagat ttttgatgaa tataaaagtg taattcatta
    10621 tgaatattaa ggttaaaaaa atatgttaat gaactttgaa gtttatcaac aaagaatatt
    10681 aataatatat aataaatgct atcatttaaa agctgtaggt aaaaatctac agctttttat
    10741 tattgtagat taaaaagatg cgtaattaaa ttttaaattg gagaaaataa attggaaggt
    10801 atcaattata aatattagaa acgttgtcat agaaaaaaac tatattaaaa aaaagtacat
    10861 agaaaaaact gattagacaa taaatacatg ctttgataat attttaaatg gagtgaaaat
    10921 gtgtagaatt ataatgaaaa aagaaaataa aaatgaaaaa agaaaataaa atggagggaa
    10981 taaaagtggg tatgttaaaa aaattatgtt cactagctat ggtagcaatt atgactacaa
    11041 cacttataac agggtgttca agtggaaatg gaaaagattc aaaaaaggat ggcgaaaaag
    11101 aaaaactagt tctttggatg ccaccagtag aaaataatat gaaggaagtt tgggacccta
    11161 ttttagataa atttgaggaa aaaaataact gtgaagtaga tttacagata attccttggg
    11221 ataattattc tgaaaaattt gcaacagcta taagtgctgg cgaaggtcca gatgtaggat
    11281 atatgtatgc agaaatgtat ccacagttta tagaaagtgg agcagtagaa gatttgacta
    11341 actatgcaac taaagaggat aaagagcaat ctatttatat aaaaacatct gaaatgatgg
    11401 gtggaatgta tggtatgcca ttccaagcag caaatccagg ggtattatac tataacaagg
    11461 atattttaga gaaactagga gaaaaacctc ctaaaacatg ggaagatttt aaaagaattt
    11521 gtaaaaaagc aacgcaagat actgatggtg atggaaagat agaccaatgg ggattagctc
    11581 agggatgggg agctaaaact tttggtaact taaactggaa ctggtatcct tatttatggc
    11641 aagctggtgg agatatattt aatgatgatt taaaatcggt taaatttaat gataagagtg
    11701 gtttagaagc agctaagttc ttaaaagaat tacaagcata tgtgcctgaa gattctttat
    11761 ctaaagatag taatgaaatg atagaaagtg tatttggacc agggaaagca gcatttacaa
    11821 taatgttatc ttctgctgca acaagtgtat ttgatgaatc attcccggat ttaaattggg
    11881 gatttgtaac tggactagaa gataagaaag cagcaacttt tggagcagta gaccatttat
    11941 ctttaatctc atctgcgaaa aataaagaac ttgcatacaa gttaatacaa catatgttaa
    12001 gtgttgaatc aatgacagaa tttcataaag caataccaag agcaccaata acaaaaggtg
    12061 aaccatatca aggtgatgaa agattcaaag aaatggtgga aaatgacaaa aatgtatata
    12121 gaccattagt agtaggacct catggagttg aaatatatga atatttatgg aaagaacttc
    12181 aaactatgat ttcaggagat aaaactccaa aacaagcgtt agatgatgct gcaaaatatt
    12241 caaatgattt attagcacaa taaaagttta aagctgtaat atgttgtaaa gtactttaca
    12301 gattaaaatt agcattgtat ttataaatac aatgctaata tttatattgg agaggataaa
    12361 atggaaaact tagttagaga gtttgaagaa aattcaaaaa gaaaaaaaac aaaattaaat
    12421 attaaaccat atatgtatat attgccattg ggaataattt tagtggcgtt ttatgtgata
    12481 ccaataataa tgtcaatata ctttagtttt acaaagtata atattattag tccagctact
    12541 tttataggtt tagaaaatta taaaaaatta tttacagatg aaacattaaa agtatctatt
    12601 ataaatacta ttaaatttac tgttgttgta gtaccttgcc aaactatatt atcattgatt
    12661 ttggctgttt ggattacagg aaaaggaaat agtaaaatag cttcatttgc taaaggtgct
    12721 atatttatac cagtattatc atctatggtt ttgataggta tggtatggag ggctttattg
    12781 aatggagaag gttctattat atatcaggta ttaggtgcct ttggaataga atcatctaaa
    12841 cttttaggag atagtaagac agcattacct acacttatgt ttatatctat gtggaaaaat
    12901 atagggtatt tcatggtaat ttatatttca gctataatga atttaccaaa acactgctat
    12961 gaagtagcga aggttgatgg ggctacaaaa cttcaagaat ttataaaaat aactgtacct
    13021 ttattgaaac caactacaat aatggttgta tttttagggt cgatatggtc attacaagta
    13081 tttgacttaa tttatactgt gacaggtgga ggtccaggta tatctactat gagtatagtt
    13141 atgcatgcat ttaacttaaa ctttaaaaac tttaattctg gatatgcaat gactgtagca
    13201 aacgtattat ttttgttaat agcagtagtt tcgattttac aaaataagct tgtaaaacga
    13261 gacaattcag atttttagaa aggtaggtat aatatgaaaa cagcaagaag attattaagt
    13321 gatggtttac ttttatttat agcgtgtgca agtttggtac cttttatata catgttgata
    13381 atatcattga agataacata taactcatat agtttggata tatctttttc aacagtaacc
    13441 cttcaaaact atatagatat ttttactaaa aaaggatttg cacaatattt ttttaataca
    13501 gcaatagtat ctttttcagg tgtattatta aatttagtat ttagtacatt agcaggatat
    13561 tcttttgcga agatggattt taaaggtagt gataagttat ttttatttat gataatgact
    13621 ttaattatac cttcccaagt tacaatgata ccactatata taataatgaa acacttagga
    13681 tggataaata gttatcttgc acttattatg ccaataccaa ctgcatttgg cgtatttata
    13741 atgagacaag ctattttagg agtaccaaaa gagttattag aatcagcaaa gatagatggt
    13801 tgttcggatt ttagaatttt aatacaaata gtgttgcctt taattaaacc agcattaatt
    13861 acactagcaa tatttacttt tatgggagca tggaacgagt ttatgtggcc tcttatagct
    13921 acaactaaag atgcaatgag aacattaaca gttggattat ctactcttaa gacattccag
    13981 ataactaatt atggacagat gatggcaggg gctacaataa catttttacc tccatttatt
    14041 ttctatttga ttttacagag taaatttgtt gaaggtgtgt ctttgtctgg tataaaaggt
    14101 taaatagtct aatttaataa tatataaaga aacttatata aatatggagg aatttaaact
    14161 atggaaattt catatgtact gaaagattgc acattcaaac acaataaata ctcagttgta
    14221 gaagctgaaa cttggaaaga taaggatttg gatattgtag tagctaaagg tgaaaaattt
    14281 gcatttcaaa ttatgttaaa agcaacagaa gaatttaatt gtacaattga taaaagtagt
    14341 gctatatcat ggaaaggcct gggaaataga gtaaggttag cattaaatat gcctaatagt
    14401 ttagaaaata attttagtat aaatatatta ggatatgttc aagatgatac aaaggcattt
    14461 gtgaatgatg ctattttaag ggataaagat gtgttagtag agcaatattt gcctcaaaca
    14521 ttttggattg aaggtcaagt accagaagat tttgaagaaa ataaccttga tataggtatt
    14581 gatatattta aaagctttgg atatgaagat gaagaaaaag tttgtacaat tgatgttcct
    14641 gtaaaagtaa gaaatgttgt gctaaagcct ttagatgaaa gcaagttctt tttagattta
    14701 tggcaacatc caagctgttt agctagaatg tataaagttg agttatggtc tgatatacat
    14761 tttgaaatag tcgataacta tttaaaagaa ttagcatctt taggagaaaa agttgcaaca
    14821 gttatagttt cagattatcc atgggcaggg caaagttgtt ataaagttta taaaaatcca
    14881 tcaaacttat atgaatataa catggtaagt gtatcaaaag gattggatgg aaagattaaa
    14941 tgtaactttg agagtatgga taggtatata ggcatagctg ataagtataa aatggctaag
    15001 gagatagatt tatttggtct attaggtaat tggtgtgctg gtgagtttgg aaatcctgta
    15061 gaaggttata aggaccctat aagagtaaga tattttgatg aaaaggataa agtatttaaa
    15121 tttataaaca atacaaatga tttaaaagaa tatataggat tagtattaaa tcatttaata
    15181 gagtgtggat tatgggacag agtaaggatt atagctgatg aaccaaataa tccagaagta
    15241 gtcaaagagt gtatagaatt tataaattct actgtaggta ctcatcaagt taaatataaa
    15301 tcagcaactc acgaccaaaa ctttttagac agagctaaag atgaaattga tgatatgtca
    15361 ataaatttaa aacttactat acaaaattat aaagatatag aaagtcttaa gaaaaaaatc
    15421 aatgacaagg gtgggatatt gacttggttt gtatgctgtt ttccagaaaa accaaatagt
    15481 tttttaagtt ctccttttgt agaaaataga ataataggat ggtatactta ttactttggt
    15541 cttgatggat ttttaagatg ggactataat ttatggacag aagacccttg gaaagattca
    15601 agttacaaat tccctatttg gaaagctgga gatatgttct ttgtttatcc tggaaaagat
    15661 ttaaagccag ttagaagtgt cagaatggag aatttaagat ttggaataca ggattttgag
    15721 ttatttacaa tgttagaaaa agaaaaggta agagagtata tagaagttga gcttatgcaa
    15781 gaattattaa ataaaaaaga aaatgctgaa attaaaggtt ttggtgacat agaactagga
    15841 tattcacttg ataattggag atatgataaa gttaaaaaat atgtattaga gttgttagac
    15901 agagtataaa aaatataaga gtgtctcaaa atgaactttt tagttcatga ggcactcttt
    15961 ttgtatgaaa tcaatatagt ttcattgttt caacaatgaa aaataggatt atttctattc
    16021 taagacagcc ctttagtttt ttagttgtat tcaatgtttt aaaactaact ataaacgcta
    16081 taaagtttat aaatgacaat aattatatat aacatgatgt actttaattt gctataatag
    16141 ggattgaata atattatatg gaagataaca ttgtttgggg ggattaatat aaaaagaata
    16201 ggtattatta taatgttttt tttaagtata tcttttattg gattttatct tgtaataaga
    16261 gataacagtg atacagttaa gatgcaacga gggataaata taggtaatgc actagaatca
    16321 cctaaggatt ttccgtggga tgtaaaaatg tcaaataagt tttttgatga tataaaagat
    16381 gcaggttttg acacagtacg tataccagta aggttttctg attatacgtc agattctgat
    16441 aattttaaaa ttgatgaaga attttttaaa aagattgata aatatgtaga ttatgcattg
    16501 gataaagact taatagttgt gttggattta caccattttg aagaaattat gaaagaacca
    16561 agagttcata aagaaaagtt tttaaaaata tggcaacaga tagctaatag gtatcaaaaa
    16621 tatgataaaa aattggtatt tgaattatta aatgaaccta aagaaaattt atcttcacaa
    16681 ttactaaacg aatacattga agaagcaata aagataatta gaaaaactaa ccccaaaaga
    16741 actattatag taggaccata taatttctat caaattgatt atttgaatga attaaatatt
    16801 cctaaagatt ctaatatagt agtttctttt cactattacg agccaaatga ttttgcattt
    16861 caaggaaata tctatcataa gggatttgag cacttaagca atattacttg ggaaggaaca
    16921 aatgaacaaa tggattattt aaaaaaaagg tttgatactg tggaaaattg ggcaaataaa
    16981 aataaggtaa aaatcttctt gggtgaattt ggaataacaa aagaggcacc agaagtttca
    17041 agaagagcat ggataaaagc tgtaagagaa gaagctgaaa aacgaaactt ttcatgggct
    17101 tattgggagc ttgcatctgg atttggtata tacaatcaaa ttgaagggac ttgggataga
    17161 gatatattaa gtgcattaat agaaaagagg tgattttatg aagaaaaatt caaaaaagat
    17221 acttataact ttaactatta ttactaatgt catatatata ttgtggagaa ttttttacac
    17281 agtacctaaa gaagaaggga agtttgcctt aatatgtgcc ataatacttc tttttgtaga
    17341 gataatgggt atgatggaaa tgtttgtgca ttattatggt atgtcaaata ttgagtatcc
    17401 tgaaaaacca atcataagtg aagaacttta tccacatgta gatgtattta ttgcaacata
    17461 taatgaatct gttgatttgg ttagaaaaac tgtaaatgga tgtattcata tgcagtatcc
    17521 agataagaaa aaggtacata tatacatatg tgatgatgga aatcgtgaag aaatgcgtat
    17581 tctagcagaa aaaatgggtg taaattacat aactagaact gaaagagaag gagcaaaagc
    17641 tggaaattta aataatgcaa tgcaacatac aacttctcct ttgattgcta catttgatgc
    17701 agatatgata ccaatgcatg actttttaat tgctacagta ccatactttt taaaaaatga
    17761 gcaagctaaa aaagatggag aaaaagagga gtatgaaaag gttggttttg tgcaaactcc
    17821 acagagtttt tataatccag atttgttcca atttaatctg cactctgaag gaaggattcc
    17881 aaatgagcaa gattattttt acagagatat tcaattggct agaaatagga caaattctgt
    17941 aatttatggt ggtagtaata cagttatttc aagggaagca cttgaagaag ttgatggatt
    18001 ttatacttat tctattacag aagattttgc aacaggtatc ttaattcaaa gtaaggggta
    18061 tagatgttat gctattccag aagtacatgc atcaggttta tctcctacag atttaaaaag
    18121 tcttattaag caacgtgaaa gatgggcacg tggatgtata caaactggta gacgtctaaa
    18181 tattttgttt aggagaggtt taggtttttg gcagaaaata agttatattt cttcaattac
    18241 ttattggtat gcaagtataa aacgctttgt atatataatg gcacctattt tattttctgt
    18301 gtttaatgtg attgtagtaa aatgtacgtt acttcaagta ttggtatttt ggttgccaat
    18361 gtatatttta agtagtttat ctcttaaaat attttctcaa aatataagaa atactagatg
    18421 gaccaatata tatgaaacta taatgtttca gtctttaatg ccagcagtaa tacttgaaac
    18481 gtttgcaata tctaaaaata aattttcagt aacaaataag agtaaattag aagaaaatag
    18541 aatgtataag tttttacagg gaatacctta ttttatttac atggtcttat ctatcatagg
    18601 aattttaaaa atgtttgtag caatatttaa aatgagttca atgacatatt cagttgtatt
    18661 attttggttg attggaaacc tttttaacct tgttatggct acattattta tatcagggag
    18721 acagcagtta agaaagtctg agcgttatat tgctgaaatt gattttaagt taaagcaaaa
    18781 ttcttatgtt ctttcttcaa aaacaattga tatatcagaa aatgggtttg catttttgct
    18841 agaaaatcca gaatatattt caccagaaga agagtttgag gtagagttta gagaaaaatc
    18901 tggaaatgaa atgtatatag ccaatatgaa agcaaagatt gttaatgttg tggaagtaaa
    18961 ttcaaagtgg aaatatgcag cttatattac acatattgaa gatagtgaaa tagataattg
    19021 gatgtgtatt gttcacgata gaatacctac tttaccaatg acaatatcta atcaactagg
    19081 tttctttgat gatttgcaaa ttaatgtgaa aaaacgtatt gaaaaaacta gaacattatc
    19141 acgaagaagt ccaagaatca atatgaattt tcagatggat ataaaaaata caggtaaatt
    19201 aagaatagtg aattttaatt atcaatatgt attattaaat tttgagaata aaagtattta
    19261 tcctaaagaa atagcattgg aaattaatga aaatattgtg ttagaatgtg atttgtgtga
    19321 gggtaaaatt gatgaaagag gtatcttata taaagtaaat aacattgaca gcataatgca
    19381 aaatttcttt ttaagagatg aaatgatgga ttggatatta caaaataaga caattcttgt
    19441 ttcaaagcct agtgaaaaga aggaaaaaag tatagatgag tttgaaccca tggaatatat
    19501 ttaggagaga tgaaatgaag actttaaaag tgtttttttg ttctacaaaa tatcttattt
    19561 tggtgtttat tttttttata aatactactt ttataagttc agcaacaagt gataaaattt
    19621 tagatttgtc ctttaaaaag atagagactg acttaaattc taagattaca tatgaagata
    19681 caggtgtaaa aatagaaacg gatagttcta aaagcgataa ggaacgttat ttatatattt
    19741 accaaaatat aaaagaaaat tggagcatgt ataataattt ttatattgaa attcaaaata
    19801 aaaataaaag ttctcagaga ataaatttaa gcatacaaag taaaaatatg cttgaattta
    19861 gattaaaaga aggtagtgaa gtatttttag aaggtaaaaa tataatatat tcagataaaa
    19921 taaaagaagg atgcatagaa gtaccaggtg aatttgaagg caaaatttat gtgaacttta
    19981 atagccttat aaatgaggaa agtaatgttg tgctggactc aaatatgctt tcaaacattg
    20041 ttagttgggg aattacgttt attccatctg atgaagaaca taatattgtt attataaaaa
    20101 agatatcttt attgtctgaa gagaaattaa gatttttaaa taatattaaa ataattggcg
    20161 atgaagaagt acaaatacca gtattgggtc aaagtataag tcagtatgaa gtattaggtc
    20221 ttaaaagtga ttctaaaatt aagtactctc ttatgggaaa acaagataat gtaagcatat
    20281 ctcaaaaagg taaattaaca ttaagtaata aagccaaacc tggacaaatt attttacaag
    20341 ttaatgttga tgataagttt aaaattggaa agaaaataac attaactgag tcttggagtg
    20401 ttaataaaaa agataaggat ggtgttccat atacattagt atatcctgag caatcaccaa
    20461 cagtacaaga tatgaaaaaa ataaacttta tgaataacat cataacgttt gttcgaattt
    20521 tatttgtatc tctagtaata atatgctttg gaatatattt atattggaaa aaatgttcaa
    20581 aaactaagta aagagaggag gttagattgt gttattttca agtttaattt ttttatttta
    20641 ttttttacct ataactttgg ttttatatta tgtatttagg tttaaccgaa cgatacagaa
    20701 catgatattg cttgctgtta gtttattttt ttatgcttgg ggagagccaa aatttgttgt
    20761 tatcatgata gtttccataa taatgaacta tatatttggt ttactggtag atagatatag
    20821 ggaaagtaaa ataaaagtaa aagttttttt agttttgatg tgtgcataca atataggtgt
    20881 tttatttata tttaaatatc tagcttttgc gctaagaaat attagtgaac ttattagtac
    20941 tgaattgact atacctagta ttgttttacc cataggaatt tcatttttta catttcaggg
    21001 aatgtcttat gttatagatg tatatcgtag acatggagaa gtccaaaaga acccttttta
    21061 tgtgggattg tatatagcat ttttcccaca attaatagca ggtcctatag ttagatatga
    21121 gagtgtggca gaacaaattc tcaatagaaa agaaacttgg aataaatttt ctattgggac
    21181 ttgtaggttt ataacaggtc taggtaaaaa agtattaatt tcaaataaca tggctatagt
    21241 agcagactat atttacacta tgaatagtca aggagaaata gctgcatcac ttgcatggct
    21301 tggttcaata gcctatactc tgcaaatatt ctttgatttt tctgcttatt cagatatggc
    21361 tataggctta ggtcttatgt ttggttttaa atttgaagag aactttaatt atccatatat
    21421 atcaaaatca attagtgagt tttggagaag atggcacatt tctttgggaa tgtggtttaa
    21481 gagctatata tactttccgc ttggaggttc tagagtagca aataaagata ttatgattag
    21541 aaatatgttt atagtgtggt tatttactgg aatatggcat ggagcagaat ggacatttgt
    21601 aatttggggg attttaaact ttgttttcct aattatagag cgatttataa tgtttgaaaa
    21661 gattgaaaat cacaatttta taaaacatat ttatgcttta ttagtagtta attttgggtg
    21721 ggtattattt aggtcaccta atttaaaaga agcatataat tattttaaag ctatgtttgg
    21781 aggcaatgga gctatatgga gtgattatac atatatgttt ttaaaagaat actgggtttt
    21841 ctttatattt gcatttattt ttagtgtacc tattgcaaag aaaatcaata agtttgttgt
    21901 tgaacaagct aaatatagta tgatatttaa cgtattttat ccaatctcag ctattttatt
    21961 gttttttatt tctgtgacat atttagtaac tggaagctat aatccattta tttattttaa
    22021 tttttagttg ggaggaaaag tatgaataga ttttttaaag taagattaat aactgcaatt
    22081 atgttcttaa ttattttatt cgctttctct atctttaatg tttgttattc tggaaaagat
    22141 acgatacagg aaataaaaaa ttcaatagct gaaaaaagta gtttaaaaga tacaatatca
    22201 tctattgata atacagtgaa tgacaaagta atatacaaaa atacgttcat tgaaacttat
    22261 ggctatttac aaaaattaat gggtaaaaat gagttttcaa aatttagtgt tgtaaaagat
    22321 acttctggaa agatgcatta tacatatttt gctaaagctc caaatccagt tggtgtgata
    22381 gcagataggg taagaaaatt ttctgatgaa atggaaaagc aaaacactaa attggtttac
    22441 ctaatgactc cagataagta tataaaagga gtgacaaaat ttccaaaagg aataccttat
    22501 tcttatagta atgagacagc agataatttt ttaaatcaac taaaaaagta taatgtggac
    22561 tatattgatt ttagagaaaa tattttaaag agtggtattc caaaagatga tttatttttt
    22621 aaaacagacc atcactggaa agtagaaact tctttttggg catttggaga attagttgag
    22681 caacttaata aaaaatacaa tatgaattta gatgaaaatc attattatag agataaggac
    22741 aattataatt caattgtata tcctaagtca tttcttggtt ctatgggaag aaagactggt
    22801 atcttatatg gtggtataga tgattttaca ttaatttatc ctaaatttaa gactaattat
    22861 acttattata cagattctaa atctcaaaaa tttgaattaa aaggaaggtt tgaagagagt
    22921 ttaatattat catatccttt taatgcagac cttgatttaa tggatggtca atcagataag
    22981 tattttacat atttattggg gaacagacca ttagttaaaa taaaaaatat agaaaatcca
    23041 gatggattaa aagtgctgtt tgttaaagat tctctaattg taccaacagc agcatttttt
    23101 tcctctgttt gttcatctat agatatgata gaccctagat attatgatgg agatatacta
    23161 gaatatgcaa aatcccatga ctatgacttt gtttttatat ctgtatatcc ccaaaatctt
    23221 acaaaagaat tttttccgtt ttgtgagtaa atttctaaaa aagttttctg ataaaataaa
    23281 tggctaactg gtaaaaagtt agccatttat tttgagtgtt attctaagaa atattttttt
    23341 tactaaatac ttctttatct atttttccaa gtgattttcc tacagtaatt gatgatacca
    23401 tagcaccatt aacatttatc atagtacgtc ccatatctag tatagggtct attgcaagta
    23461 taccaccaac taatggaaaa taagaaccaa gaccaactcc agagataaca actgaaacgg
    23521 ccattgtagc agttcctgga agaccagcta ttccaaacga actaattgtt ataacaatta
    23581 acaacataac ataaaagctc atatccatgt ttactccagc catattggca agtgttacag
    23641 ccattaaagc aggatatata cctgcacaac cattcatacc catatttgca cctaaacttg
    23701 ctgtaaagct tgatacaccc tcatcaactc cgtgattttg tactagactt tctatcgtta
    23761 caggcaatgt tcctaaacta gaacgtgaag taaatgccag aagtaaagct tcagttgaat
    23821 tttttatata agtaatggga tttagaccat ttaaagttat aaaaattaaa tgtataataa
    23881 ataatattgc aactgatata taaagagcta atataaactg taaaacagat actattgaac
    23941 taataccttg tgaagttatt gtatttgcaa gcagagcaac cacagcataa ggcataaatt
    24001 taataacagt catagacaca cttacaatta ttttgtaaga tgcttctata aaacctataa
    24061 atggttttat tgtttcaaaa tgttttttac ttaatctctt tattgaggta gctataaatg
    24121 tagcaaatat tattgtagct acaatatttc catttgccat agccattaca ggattagatg
    24181 gtagtaatcc tcttaaagta tccacaagag agtttatttc tttaagttct tgattttcaa
    24241 ctaaagtagg catattagct cctacaccta acttcattat atttgcaacg ataattccaa
    24301 caattgcagc tatagcagta gtagcaagaa gcatacctaa agatttaaat gtcagagttc
    24361 caagattgtt atctttcata tttataatta cttttataat ggataagaaa actaaaggta
    24421 ctactaacat ttttaataag tccataaatc cagaaccaaa taggctatac cacttactta
    24481 cttcttgtag ccaaatgact tgagttgtat cattaggaaa accagcaaaa gcttgtatta
    24541 tgattcccaa tatgagtcct agtactgtag catatattgt tctcaatgaa aattttattt
    24601 ttttcttttc aaattgtcta acaataaaga acgtacctag aagtaatatt atgaaaaaaa
    24661 ttgttttaaa atctgtaatc ataagaaatt gtgataaaaa tgaactttcc aatatttttc
    24721 tccttttatt taaaaataaa tttttctaag tttaaatgat atcaatatat taaaaaaatg
    24781 tcaatattat atcacaattt acttaaatag agtaaaacaa gttgattttg aaaaatgtga
    24841 ttaagtacag aaaaataggg tataataaat gtttaaagaa tttgaattgt ggtaagaaaa
    24901 taaaaaatag cttttataag atttgatttc aaattaaaat gtgttaaata aataactaaa
    24961 aattattata aaaaatgttt tcatagaata tatatcaaat gaaaagctta aaataagtga
    25021 agaaattaac tagacatcaa aggagagata aaatgagagt aataataatt ggtggtgttg
    25081 cagctggtat gagtgctgca gcaaaattaa aaagaataaa acctgagtat gaagttgttg
    25141 tttatgaaaa aactgaaata gtatcttttg gtgcttgtgg attaccatac tttgttggtg
    25201 gattttttaa tgatgctgat gaattattag caagaacacc agagaaactt agagaagctg
    25261 gaattgattt aaatatattt agagaagtcg ttgaggttga tagtgaatct aagaaaataa
    25321 aagtaaaaaa cataaaaaca gatgaaatat atgaagatta ctatgataaa ttgatgatag
    25381 caactggagc aagaagtatt atgcctccta taaaaaacat aaaattaaag aatgtttcta
    25441 cattaaaaag tttatatgat ggagaatatt taaaaaaact tttaagtaat gaagataata
    25501 aaagagtgac aataatagga gctggtttta tagggttaga agcagttgag gcatgtaaga
    25561 aattaggtaa agatgtagat gtaattcagt tagaagatag aatattgcca caagtatttg
    25621 ataaggaaat gacggatgta ttagaagaag aaataaatag acataatgtt aatttgcact
    25681 tagatgaaat ggttgtagaa ctttgtggag aagataaagt agaaaaagtt ataacaaaca
    25741 aaggggaaat agatacagat gtagtaataa ttgctactgg cgtgagacct aataccgagt
    25801 ttttgtcaaa tactaatata aaaatgctaa aaaatggagc tatagtagtt gatgagtatg
    25861 gaagaacttc tgtagaagat atatactctg ctggagattg tgcaactata aagaatatag
    25921 taagtaatga aaatgtatat gttcctttag ctactggagc taataaattg ggtaggatag
    25981 taggagaaaa cctagcagga agagaaatat cttatcaagg ttcactatct tcaagctgta
    26041 taaaaattat ggatatggag gcagcatcaa ctggaattac agaaaggcaa gctaaagatt
    26101 taggcataaa tgtcaaatct aaatttattt ctgattataa tcaaactcat tattatccag
    26161 gaagaaataa aatatatgta aagcttattt atgatgcaga tacaaaagtt attttaggcg
    26221 gacaagtggc tgggtttaag gatgctgtcc aaagagcaaa tgtattggca gctgctatct
    26281 ttggaaaaat gaccacaaat caacttggaa tgttagattt atgttatgct ccaccatttg
    26341 caagaacatg ggatgtttta aatgtagctg gaaatgtatc taaataatat ttagattata
    26401 gctaatatag ttgacaagca attaagggaa tgaatttacc taaactattt ttatcatggt
    26461 aaaattcatt cctaaatttt tattccaaat ttttattcca aatttttaga aataaggatt
    26521 ccattaaatt attcatcaaa atttaaacgc aagcttaatc tatcttctcc aagaatagta
    26581 ttttcaaaaa cattagtagc attttctaaa tacatactag gtatgtctaa agaaggacta
    26641 aaatgagtca gtaaaagttg tttgacattt ccaagttttg caagattagc agcttctcta
    26701 aaagtcatat gtttgtttct aacagctttt gatatatcta aatcatcacc atacatggct
    26761 tcacatataa ataaatcact atcttttatg aaatctggta ttgattctat aggtcttgta
    26821 tcggttgtaa gacttatctt aactcccttt ctcaattctc ctaagaccat atctggagtg
    26881 tattgttttg aatctaaaac tatattttga ccttcttgga gtttgttcca taatatttta
    26941 ggaacttcgt ttttagttgc cttatctata tcaaatttag ggtttcttct aaaattaaaa
    27001 ctgtatccta aacactctat agagtgttct aaactgattg ttgaaatttc taaatttctt
    27061 aaaactttat tatctaaaga aaaattgcct tgagggtttt ctataatttt taaagtgtat
    27121 ggtacatatt ctactaaatt tctcatagaa cgtatacaat cgactattcc aactggacca
    27181 actatagtta aatcacttgt acgaccacta ttcccaattg ttgataaaag tcctaaaagt
    27241 ccaaatatat ggtcaccatg taaatgggtt atacaaatca agtctatatc tttaaagcca
    27301 cattttttta gcttcataga tacctgagta ccttcaccac agtcaattag tatttttcta
    27361 cctttatagt ttataaatac agaggataga aatctgtttg gcataggtac atttccacca
    27421 catccaagta aagttaaatc tatcattttt tcccctttct aaaattaaca caaatctgtt
    27481 gtttgttttt attatgatat cctattataa ttattttaac attaaatata acaataaatg
    27541 tgtatttgag tcaagaaata ttgtcaaata aagtttttat atagcaatgt ataaaaattt
    27601 ggaaaacgaa aagtgaaaat tatatttagg agtatttaaa ggaggaaaat ttaatgaaag
    27661 tattaaagaa aattactatt ttgtcattaa ttacattaac cttagttggt tgtagtaacg
    27721 gagaagagaa aaaagtggtt gaaggttcta agaaaactca aaattctaaa aaagatgaac
    27781 agcctataat agtacataaa gatgcaaata tattgaaaga taggtatgag ccaccaaaag
    27841 gatttaaaag agttgatgta gaaaaaggta gttttgaatc atttcttagg aatactaagc
    27901 taaaaaatta tggagagaaa gtaaagtatt atgatggaag aactaaggaa tcaaaaggta
    27961 tttatgatag tgtatttgat gttgatattg gaaatagaga tttacatcaa tgtgcagatg
    28021 caattatgct tatgagagca gaatacttgt atcagaataa actatatgat gaaataagtt
    28081 ttgattttgt tgatggtttt aaggcgaagt attcaaaatg ggcacaagga tatagaattt
    28141 cagtaaaaga tagtggttca agctggtata aagcaactga atattcaaca tcttatgaga
    28201 gtttcagaaa gtttatggat atagtgtttg catattcagg aacgttatct cttgaaaaag
    28261 aattggagcc tgtaaaagtt gaggatatga aaataggaga tgtgtttata gttggtggaa
    28321 gtccaggaca tgcaagtata attatggata tggcagaaaa tgaaaagact ggtgaaaagg
    28381 tatttatgat agcccaaagc tatatgcctg cacaacaaac gcaacttctt attaatcgta
    28441 aaaatccaaa tttgagtcct tggtatagtt tagattttga aggtgatttg acaatacctc
    28501 aatggacttt taaaagagac cagttgaaaa gattttaaaa taataatttt atataataaa
    28561 agaaaaatat agatgtgaaa aaatgtaatt agattaaaac taattggata tggacaaaat
    28621 taaattgata gataaatatt aaaaattac
//
LOCUS       DAYAOX010000026        27784 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.026, whole genome shotgun sequence.
ACCESSION   DAYAOX010000026 DAYAOX010000000
VERSION     DAYAOX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.026"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(50..238)
                     /locus_tag="ACGI57_000935"
     CDS             complement(50..238)
                     /locus_tag="ACGI57_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716021.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            573..1541
                     /locus_tag="ACGI57_000936"
     CDS             573..1541
                     /locus_tag="ACGI57_000936"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFG9716022.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            complement(1658..2788)
                     /locus_tag="ACGI57_000937"
     CDS             complement(1658..2788)
                     /locus_tag="ACGI57_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9716023.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(2941..3894)
                     /gene="msrA"
                     /locus_tag="ACGI57_000938"
     CDS             complement(2941..3894)
                     /gene="msrA"
                     /locus_tag="ACGI57_000938"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFG9716024.1"
                     /translation="MIKLATFSGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            complement(4018..4923)
                     /locus_tag="ACGI57_000939"
     CDS             complement(4018..4923)
                     /locus_tag="ACGI57_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFG9716025.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            complement(5295..6887)
                     /gene="hcp"
                     /locus_tag="ACGI57_000940"
                     /gene_synonym="priS"
     CDS             complement(5295..6887)
                     /gene="hcp"
                     /locus_tag="ACGI57_000940"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFG9716026.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(6947..7672)
                     /locus_tag="ACGI57_000941"
     CDS             complement(6947..7672)
                     /locus_tag="ACGI57_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9716027.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASFLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            7837..8553
                     /locus_tag="ACGI57_000942"
     CDS             7837..8553
                     /locus_tag="ACGI57_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9716028.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFQNKLDI"
     gene            complement(8678..9253)
                     /locus_tag="ACGI57_000943"
     CDS             complement(8678..9253)
                     /locus_tag="ACGI57_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9716029.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHIPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNHYKTIVVT
                     KAFERNSTKKNFKVLKKAYKLGMKIAIDING"
     gene            complement(9427..9849)
                     /locus_tag="ACGI57_000944"
     CDS             complement(9427..9849)
                     /locus_tag="ACGI57_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9716030.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            complement(9863..12040)
                     /locus_tag="ACGI57_000945"
     CDS             complement(9863..12040)
                     /locus_tag="ACGI57_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFG9716031.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYKQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEANCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            12321..15500
                     /locus_tag="ACGI57_000946"
     CDS             12321..15500
                     /locus_tag="ACGI57_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFG9716032.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGINKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLDKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(15630..15764)
                     /locus_tag="ACGI57_000947"
     CDS             complement(15630..15764)
                     /locus_tag="ACGI57_000947"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716033.1"
                     /translation="MKTVKWIAKLTKVSVHILHYYDEIGLLKQNILIVKLLHLYMQYV
                     "
     gene            complement(15785..16549)
                     /locus_tag="ACGI57_000948"
     CDS             complement(15785..16549)
                     /locus_tag="ACGI57_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9716034.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIINFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(16705..16992)
                     /locus_tag="ACGI57_000949"
     CDS             complement(16705..16992)
                     /locus_tag="ACGI57_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716035.1"
                     /translation="MLCYRNEVAKNTIEVVKDLKAEDMKRKVSPIDLERILVTGGVIK
                     HEDSIWLLDFWGNKDIAGILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(17012..18505)
                     /locus_tag="ACGI57_000950"
     CDS             complement(17012..18505)
                     /locus_tag="ACGI57_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFG9716036.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            complement(18833..19375)
                     /locus_tag="ACGI57_000951"
     CDS             complement(18833..19375)
                     /locus_tag="ACGI57_000951"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9716037.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            complement(19665..20612)
                     /locus_tag="ACGI57_000952"
     CDS             complement(19665..20612)
                     /locus_tag="ACGI57_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716038.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGYIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            complement(20640..21131)
                     /locus_tag="ACGI57_000953"
     CDS             complement(20640..21131)
                     /locus_tag="ACGI57_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716039.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            complement(21574..22467)
                     /locus_tag="ACGI57_000954"
     CDS             complement(21574..22467)
                     /locus_tag="ACGI57_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFG9716040.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            complement(22692..24977)
                     /locus_tag="ACGI57_000955"
     CDS             complement(22692..24977)
                     /locus_tag="ACGI57_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFG9716041.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLLYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKCIKIDK"
     gene            complement(24974..25654)
                     /gene="pgmB"
                     /locus_tag="ACGI57_000956"
     CDS             complement(24974..25654)
                     /gene="pgmB"
                     /locus_tag="ACGI57_000956"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFG9716042.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            complement(25878..27761)
                     /locus_tag="ACGI57_000957"
     CDS             complement(25878..27761)
                     /locus_tag="ACGI57_000957"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFG9716043.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
BASE COUNT         9297 a         4546 c         2905 g        11036 t
ORIGIN      
        1 cttatataat aaaatgtata ttaaaacatg ttaaattgca aaataagctt caatctcttt
       61 tactaatttc atccattatt aatgattttg aaatatacat tacttctata atattcttaa
      121 aacgtgtatg atgagatgtc tcctcagaaa atttcttctg tccttttcca caattaataa
      181 tacttgaaga tactactctc agtgcttcca ccaattcttc ttttgtatga taattcataa
      241 cacatttact cctttatttt ttaatttaga gaaacagtat atctcaattt aatatcatta
      301 taaactgaca tcattattat ttatacacat cttttagcac ttaaatattc taactatcag
      361 tgacattaca tattttaata attattaata tacatataat actttaatat tcactaatag
      421 agagtatatt aacattgctt ttttaaaaat tttagtataa aatcatatta aataaatttt
      481 ctgaaataat agaatattaa aatatccaaa aacagctttt aataacatct ataattttca
      541 atgtatcgtt attttagggg gagtgattta aaatggcaat aaagccaaga aaaatatcta
      601 ttgtaggttc agggcatgtt ggttctcatt gtggatttag tttaataact caaggagttt
      661 gtgatgaact ctttatgatt gatatagatg aatctaaatc aaaagctcaa gctttagatt
      721 tagctgatgc agtttcatat ctgccacata aagtacatat agaaaaagga actttttctg
      781 actgtaaaga ttctgacata gtagtgataa gtgttgcaga ctcttcagaa gggcctttac
      841 gtagacaaaa cacaactaga ttagatttac taagaccaac tattggaatg ataaaatcta
      901 tagtaaaacc catagttgat tctggctttg atggtatatt tgtagtcata tctaatcctg
      961 ttgatgtagt tacaaattat atttgggaaa aatctggctt tcctaaaaac aaagtcattg
     1021 gaactggaac agctttagat tcaacaagac ttagaaggat tttatctgaa gaaactggaa
     1081 tcgctcaaca atcaattcaa gcttattcta tgggtgaaca tggagattct caaatggttc
     1141 cttggtctca tgtttcaatt ggtggtaaac caatcttaga catgataaaa gacaacccaa
     1201 gtacttatag caatttagac ttaccatcaa tagttgaaaa aaataaaaaa acagggataa
     1261 gtataataaa tggtaaagat tgtacagaat ttggtatagg tactgcttta gtagaaattg
     1321 taaaagctat actacacaat gagaaaaaag ttcttccagt ttctacatta ctagaaggtc
     1381 aatataacga aagaaatgta tttgcaggtg ttccatgtgt tataggaaaa gatggtattg
     1441 aagaaattat tgaaatcaat atgactgaat atgagcaaaa tgaatttaat aaatcttgtt
     1501 ctgttttaag ggaatatatt gcactttcaa aaacacttta gattcaatat tctttcttac
     1561 actaaaagca ggtaaatgac ttattgaaaa gacctttaac taagttaatg tttaaatcat
     1621 ttgtacctgc ttttaatttt aatactaaaa tataaaccta ttttttacca tcaaatttta
     1681 taactttact ttttttattt acctcttgag ataacaatcc ttttttcaat cccatcaata
     1741 attctaagtc ttctggataa agatttattc caaattcctc taaacttgat actatactat
     1801 ttacactcat tattttattt tctatcaata attcaactgc tctttttaaa tacgttgggc
     1861 ttgaaccttt cattttgtct agtggttctt ctatcaacca gcctttttta tccatttcat
     1921 tcataagata attgtatttt ttgtaattta tcttttctag attatatgct ctaaaaatca
     1981 tagcagatat aggtacaatc cattttttct ttagttctac ataataatct aaatcttctg
     2041 gatgagtcaa atcttttaaa aaactctctt ttggcaataa aaatgcacag gcatactcat
     2101 cttctgaaaa ttgcttactt ggtattctta attcacttga tattatatat ccaagctcac
     2161 atgccaaagt ataatttcta attgatgctg actttttgtc attacccaga gatattagat
     2221 atttagattc tttactaatt ctctgttttt gagtaaagat agttgcccct tttctatcta
     2281 cattcatacc tgatattata atccctttag actctaacaa acttaccata tttactattg
     2341 gagtactatt taaatcccaa aactctctag tcttatatgc aagagattct atatcattta
     2401 tattaatatc tgacattact tttctatttg gcaagttttc ttttggaaac ttaatataat
     2461 tctccataaa tgaatatatt ttatgtatta taattatttt ttctctataa gaaatctctt
     2521 ctactctagg tagtttagat tgagggttaa agtggctatc ctctacaacg atatttatat
     2581 tttcattttt ataaaaatac tcttttggaa aatttaatat attagataat tttaatgtat
     2641 tttctatatt tggtacatac ttattttcct caaaagcttt taaatctttt ttatttattt
     2701 tagtttcttt agacaactgt tctatcgtct ttgctctaaa aattctagct gattttaatc
     2761 tctttccatt aaagtctttt aaattcattt gttttctctc tttcaaaatt aaattcttaa
     2821 tctattggta taaataagat tacaattaat aatttatcat attattgaca aaaaaacaaa
     2881 aggtgaaaat atttttttat attttcacct tttagataag tttataataa atattcctaa
     2941 ttactcaaaa agttttaaat attctccata tcctaaagct tccatatttt ctcttggtat
     3001 aaatttaaga gaagcagaat ttatacaata tcttaaacct ccaagttctt ttggaccatc
     3061 atcaaataca tgacctaaat gagaatttcc tttagcactt cttacttcaa ctctgtacat
     3121 accttgtgag aaatcacttc tctcaatgat agatttatct ataactggct ttgaaaaact
     3181 tggccaccca catccagaat caaatttatc ttttgaagaa aataacactt ctccacttac
     3241 aatatcaaca taaattccct ctttgttatt gtcataatat tcattttcaa aaggtacttc
     3301 tgtcatatca ttttgagtga cttcaaattg tataggagtt agagtctttt ttaactcttc
     3361 cctatttata ttgtttccat cccaatggct ttttacaaaa ttatatcttc cagacccttt
     3421 ataatacata ttgtagttta atggtctttt cttataataa tcctgatgat tttcttctgc
     3481 ttcgtagaat ttagttgctg gaattatatc agtcactatt ttactatcaa atattttact
     3541 attttccaga tgttctcttg actctaaagc tatatttttt tgttcttcat catgataaaa
     3601 tatagcagtc ttatattgag ttcccctatc accaaattgt ccaccatcat ctgtaggatt
     3661 aatctgtttc cagaagatat ttagaagttc atcataactt attatatcat catcatatgt
     3721 tatttgaatt gcttctaaat gaccagtagt tcctgagcat acctcttgat atgtagggtt
     3781 ttcaacatga ccacctgtat atccagaaac tactttaata actccatcat acttatcaaa
     3841 aggttttacc atacaccaaa aacatccacc tgaaaaagta gcaagtttta tcattttcat
     3901 cactccttaa ataaattata ctttatttat tccctaagaa gtaacttaat aatctgcatt
     3961 ttattatttt tacctactca atgaaatcat gtgtaaaaat acaattgcaa attgaatcta
     4021 atttctataa aagctctttt tctttatcca tccaaaagca tcttttatat cacaattttc
     4081 tcctaaagac attagatatt ctaatgcatc acaatcatcc atagctacat ctttttccat
     4141 cttaggattt aatctatagt aattatctcc aagaagtttt tgagaaaata actgactcat
     4201 ttgagaattt ccttctaagg taatagatat aattggtaaa tcgggttctt tattaattat
     4261 ccagtcaata gctccccatt cggtagtatc tgcctttata ctgttatata catatccagt
     4321 ccctattgaa aaaagtcttg tattcttaag tttaaatcca ctgtcaatgg catgaattat
     4381 acacgctaaa ctcgggtcag ttgctattat tcctccatca acatgacgat tatatgttgg
     4441 aaaaaatacc ggagcagcac tacttgccat tgctacatct acaactttgc tagtctttgt
     4501 aaaagaattt ggcatattgt tataaaacac tggtttccat gcattttcct catttcctat
     4561 ataaaaactt ggtatcatta ctaatttatt taaatctttt aattctagtt cctctggaaa
     4621 tatacttaat aaaacttctt ttaagttttt gttttcatac tttggtctga gtatttctgc
     4681 ataactttta ttaaaaatat actttgaatt ttcaattgag taaagttctt ttatctcttt
     4741 agggcttaca ccatacgcaa gccctagtgc aattagactc cctgtagatg tacctccaaa
     4801 catatttata ttgtttaata gatttggata ctcttttact atcctctcaa gtatggatat
     4861 actcaatgcc ccttttaatc caccaccatc aaatgccaat atattaaaat tagaattttt
     4921 catatacata tacccccaca taaaacacct tatattttaa attttaatca aaataatttc
     4981 actatagtat atatatttta tgcaagttac atattacaat gttcctcttt taatatttaa
     5041 gttacattca aacctttaat acttaaaata aaatatataa gatattatat atctaagtat
     5101 tataaattta atacaaagat tcttaaaaag aatatactga aagcaggttt atttttcaat
     5161 tattacaaaa aataaataaa gggtcatctc aaaattgatt ttttaatcaa attttaagtt
     5221 taacccctta tcttaattgt ggcatactta aaaatatttt aactataaat atctttaagt
     5281 atagatatgt ttatttatcc taaaatcttc ttcatatctt cttcaggagt agatataggt
     5341 gtcaatgcaa atttatcaac caatatatct aatacattct tagataagaa tgctggcaaa
     5401 gaaggcccta tatagatatt tttgatacct aaatgaagta gtgtaagaag tatacaaaca
     5461 gctttctgct cataccatga cagtaccatg ctaagaggca aatcatttac tccacattca
     5521 aaagcatttg ccaatgaaac tgccacttta attgcactat aagcatcatt acattgtccc
     5581 atatccatta aacgaggtaa gccacctata tccccaaggt ctaaatcatt aaatctatat
     5641 tttccacatg caagtgtcaa tataacagtg tctttaggag cttgttttac aaattcagtg
     5701 taataatttc ttccaggttt tgcaccatca caacctccaa ccaataaaaa gtgtcttata
     5761 gctccactct ttacagcatc tactacttta tcttgtacag atgaaactgt acctcttgca
     5821 aaaccagtag ttaatgtatc tcctccatta ataccagtca tatgaacatc ttctctatat
     5881 cctccaagtt caagagcttt ttctattaca ctagaaaaat ctttatcttc atcaatatga
     5941 accatacctg gataagcaac tacttctgta gtaaatactc tatctctata actatcttta
     6001 acaggcatta aacagtttgt tgtaaataat attggagcag gaatattatc aaattctttt
     6061 tgctggtttt gccatgctgt accaaaatta ccttttagat gtttatattc ttttagtttt
     6121 ggatatgcat gtgctggtaa taactcacca tgagtatata tatttatacc cttatctttc
     6181 gtttgttcta ataatttgtg taaatcataa aggtcatgtc ctgtaactac aataaatggc
     6241 cctttctcga ttttcataga tacctgagtc ggttctggtg ttccataagt ttcagtattt
     6301 gctctatcaa gcaattccat acattttaag tttatttctc cagttttcat aactagacct
     6361 aaaagttcgt caatagacaa gtctcctcca actgctatta atccttcata gaaaaattca
     6421 tttacgtctt tgtctgtata accaagtacc attgcatgat atgcataggc agccatacct
     6481 cttaatccaa ataagattaa tgattttaat gaacgtatgt cctcattatc attccataaa
     6541 tccttcatat catagttatc atttctatta caagttgttg aacaatctga acatctaggt
     6601 acaagtttac ttttttctac ctcagttttt tcaatttgtc tttttatagc ttcatcatca
     6661 aaacttacat tcgttatagt agtgaatagc ccctctatca taattttatc tgtttcactt
     6721 gttggtctat tcttatttcc aatagttgct cttgaaagac caatcaacac tcctgtaagt
     6781 tcgtcttgta agttagctgt accccccttt ttaccacaga caccatttat agtacaacct
     6841 ttacctcctg ctgtttgttc acattgaaaa caaaacattt tttccatttt ttattcctcc
     6901 ctttttattt attaaaattt attatatttt tctataattc acttaattaa atttctccat
     6961 ctacaatttt tccatctaca gatatagtaa ctatttgcca tggtatcatt tttccactat
     7021 tttgtagagc tgtcttaaca gcatttacta taccaccaca gcaaggaact tccatacgtg
     7081 ttactactat atttttaata tcattttctt ttaaaatctc tgtaagtttt tcagcatagt
     7141 caacttcatc aagtttaggg catccgatta atgtaacctt atttttcata aatttattat
     7201 gaaaatttcc atatgcatac gctgtacagt ctgctgctat taaaaaactt gcattcttta
     7261 aatatgatgc attagttggt actaatttta tttgtactgg ccattgatta agttgagaac
     7321 cttttatctc tactatatca ttgcttatag acgtactatt tgaaacctct ctatttattg
     7381 acttagactg agaacctgga catccacatg ctaaagtttt cttttgtgct ttctttgctt
     7441 ccatatttgc ttttactgca gcttcatcat attctgctgc ttctctatac tcaaatgaaa
     7501 tcgcatttgt tggacaagtt ggcagacaat ctcccagccc atcacaatag tcatctctta
     7561 aaagttttgc ttttccattt accattccta tagcttcttc atgacaagct tcaacacata
     7621 gtccacaacc attacacttt tcctcatcaa ttttaattat ttttctcatc atttatatca
     7681 cctcataaaa tttttttaat ttaaagtatt gatttcttat atatttattt ttaataccta
     7741 tatttcttta tttcaaattt cttgtagtag gatttttata tccccttgct tttataaact
     7801 tagtatagta caatgaaaac aaattatcgg ttgcatatgc aacaaggaag tgattatttg
     7861 aaaaaatttt tagaaaagca tatagactta cttaaaaaaa ttcctctatt tgaaggaatt
     7921 aaaagtgatg aacttgaaga aatgttggaa tgtttaggtg ttgtagataa gacttaccca
     7981 aaagatagta taatattctc tgttggaagt gaagttactt ctattggaat tatgcttaaa
     8041 ggaagtgctc atataataaa ggaagatatc gaagggaata ggaatattgt agctgaactc
     8101 ttgcctggag atttatttgg agaggtattc gcatgtacta gactacataa aagtaatgtt
     8161 acagtaacca caacatcttc ttgcgaagtt ttatttataa agtttaaaag tgtaactggt
     8221 atttgttcat ctgcatgtgt atttcataat aggcttgttg aaaatatgct acaattaata
     8281 gctgaaaaaa acattttact tcacaataaa atagaattac tttctcgtaa aacaactcgc
     8341 gagaaattgc ttatgtattt ttcaaaacag atagaacaaa caggtagcca ccagtttaca
     8401 attccctttt caagaaatga aatggctgat tttttatgtg ttgacagaag ttctatgtca
     8461 agagagcttg gcaagatgcg agatgaaggc ttgttatctt ttaacaaaaa taaatttgaa
     8521 atcttttatt ttcaaaacaa gttagatatc taggcttgta taatatctgt attcaaataa
     8581 tattgttttt tattagttat tttctagaaa cctttataca aaaaaggagt attaataatt
     8641 aatgctcctt tagctatact ataaattaaa cttttatcta tccattgata tcaatggcta
     8701 ttttcattcc aagtttatag gcttttttca ataccttaaa atttttttta gtggaatttc
     8761 tttcaaatgc tttggttaca actatagttt tgtagtgatt taatcccatg ccctttaaat
     8821 gttttgcagt atacttaaaa tattttttaa ataaaaacga aataggagct gcttgagtat
     8881 agaccatgat taactttttt tgaccaaacc taggtatatg tttagactta tttatctctg
     8941 ttaaaggata caatctatct aaaagtagct ttgtttgagc tgatacctga catatatata
     9001 ctggcgaacc aattattata tattcagcac tttttatatc ttcatatatg tcaatcatat
     9061 cgtctttaaa tgcacaagta ggaacttttc tacagtatag acagccttga catcctttaa
     9121 tattcatatc atttagataa tattttttaa cttcaacatt attatcattt acacctttta
     9181 ttatctcatc aagtattgtg tctacattcc ctttttttct tggactgccc ataaaggcta
     9241 atacttttac catattaagt tctcctgtct aatttatttt tctaaattta atctaaagca
     9301 cactttattc taaaaatata ttttatatac tttgtatttg ttttatttac attttatatt
     9361 acaaaagtat atcaaaaaat aattcttaac acaattattt aaaagtagta tagcaattta
     9421 atacaattat ttattaaatt ttaatgctcc tgtagggcat accattacac aaggtgaatc
     9481 cataccacac ttatatctct caatacaacc attacactta tttgccttac catcatttcc
     9541 ataaattaag gctccatatt cacaagcttt ttcacaaagt ttacacccta tacagttttg
     9601 attgtctaat acaatgaatc cttcttcttt tttaaaacaa tttttaggac atacacctaa
     9661 acattttggc tcattacaat gtatacatgc taatgaaaaa tattctatat tttcaccttc
     9721 ctctattgta aatacatgtc taaaaagttc accattgtct atattattat cattttggtc
     9781 tatacatgca agcaaacatg ctccacatgc tgtacataat tctttattaa atttaaataa
     9841 acttttcatt atatctctcc atttatctat tcattttttc aatcttacac ttacaacttc
     9901 tatacgcagg gaatccacta taagggtcaa aattatcaca aataatacta tttatatcag
     9961 cttctctata tccatgatac atatgtatat tcttatataa gactctatca gtaacattga
    10021 cttttacatc caaacttcct atcttagtag ttatttttat ataatctcca ctgataacac
    10081 ccaattcttt tgcatctaag ctagaaatat cacaattagc ttcatttttt aatattcttg
    10141 aaagtttgat gtcatgaagt cttgaatgta gtgcatttga aattcttgct cctgatgtca
    10201 ataaatgagt atattcatca tctatattat cattaaatcc tctatatgtt ggtagtgcat
    10261 ccagcccaaa atctttatat ttttcaataa ttgtagattt taattcaaac ctagaagtgc
    10321 tagtatcata cccttctctt gtatatgaac ctactatata tggcttaaaa tctttacatt
    10381 ttaatggtaa atcactttct ttaagtttat ctacatctac acatgtattt ttaaacatat
    10441 gtcttacact tgcttcataa cctgctttta ataaatcatc atctatattc atagcatttg
    10501 ctaaatctga aagtatttct acatctgatt ttgaatcgta taatttatca ataacttttt
    10561 tggtatatgt aatataccct cctggataac tcttacattc ttctctctca aatgatgtac
    10621 atgcaggtaa aagtatatct gcatattttg atgtatctgt cataaataaa tctatattca
    10681 caaaaaaatc tacactattc aacgctttaa aaagtctccc tgaattagga aacattctag
    10741 catttatccc atgacaaaac aaagctctta tttcatatgg tgtcttttct tctatttgcc
    10801 taggtaaatc catagcttga aattgagtca ccatttcatt ccatagagga aatctttctc
    10861 caccaattct ttcatttata tttttaggta gaacttcttt tacaaactct ttttctagtg
    10921 tttggaatcc acctatctta ccagcaaagt aatcttgttt tggtatgttt ccacctattc
    10981 tatcataatt acctgttata gcggataaag acatcattgc tctataattt tgaaatccat
    11041 tttgatgatg aactattgga gctgcacttt catttataca aaatggacct tgtgacaaca
    11101 tctcaacagc ttttactata ttatctttag aaactcctgt aattctactt acagtatcta
    11161 aatcaaataa ttttacatat tctttatatt cattaaatcc atgtacatgc ttttctatgt
    11221 attctctatc tattaaatcc ttttgtataa atagattcgc cattccaaga gctaaagctc
    11281 catcagttcc aggtttaatt cttaaatgta aatctgctag tttttgagtt gtcggtgtta
    11341 ttctagggtc tacaattata aattttagac ctttttcttt taattttatt agattcacac
    11401 tatttaaaaa tttagagtag tatggattaa atccccaccc taaataagtt tttgcattct
    11461 ccatatcttg cattgtaaag tttcttccag tagcaacatt tccagccatc tgtgctgatg
    11521 ttgaacatga actagattct gtaccataat taggtgaacc aaatgaagat gcaaaccttt
    11581 gtaaaaaagg tctataccac tttccataac ccgtaaaaaa agctacatgc tttgctccat
    11641 actcagattt tactctattt aatttttctg ctatttctct ataagcttca tcccatgata
    11701 ttggtttaaa ttttccttca cctctttttc caattctttt tagaggtgtt tttattctat
    11761 cttctctata gatatattct ctattgcaaa gacctttggt acataacaac cctttattca
    11821 ttggatgttc ttttgtacct tctattttta ttactttacc atctttaaca tatgcatcaa
    11881 ttccacaatg tactcctggc gaacaaatat cacataaagt cctctttatt tctatacctg
    11941 tttccttaca gggtatttta gctttaagtt gtttataact atccatatta acattttcct
    12001 ttcccaataa taaatacaaa ttattatact tattatacat tctttttatt caaatatcat
    12061 actttatatt ttatacagtg taatttctaa gaatattttt aataataaga ttttatattt
    12121 attaataatc tatttatata attatttttc attcatagtt tataagataa ttcaataaaa
    12181 atctaatagt aaattgttat ttacacatat atcgtgatag ataaaagcta ttcatatctg
    12241 ccttaatttt agtcttaaat ttagagtgta aattgaatgc atttagatta taatatatac
    12301 agtgaggtaa ggtgaataat atgaaaatag atatgatata taaactaata agaagaaata
    12361 cagaaccatc aagatggtca agaggtgtaa aatattatac taatcaatta gttgattata
    12421 tagacataga tattgatgac ctacaaaata atctgttgac atttcgtggt ggagtcaatt
    12481 ctgaagataa ttatagcttt tattttaatt caataactat agatataaag cactttaaga
    12541 taattgaaaa tgattgtagt tgcgctgact gtcaatcaaa gggcaaaagt atttttatat
    12601 gtaagcatat agttgctatt gtgataaaaa gcttaaaaca aataaaaaat aaaaaagatg
    12661 ttcctcctat tctaatagat atattaggtg cagataagga taagctttct aatacaaatt
    12721 ctaaaaccag cttagattta agcaagcaat tactttcttt gttagacaat actaaaagag
    12781 aaagggttaa tttagatgta ttgataaatg tggattcaaa taaagcaatt gctgaattta
    12841 aaataggaat taataaaatg tacattttaa aaaacattac tgaatttgca tattcaaaac
    12901 ttaatggata tgaattagat tttgggaaga aatttactta caaccccaac actcattatt
    12961 ttgctgatga agatgagaag ttagtagata ttatagaaga atatgggaca aatctttcat
    13021 acaacttttc ttcatcacat tcaaaaaaat ttatggagat aaatgcaagc tctttaaaac
    13081 aatttttaaa tgctattaag tacaaggatt ttacccttat tttttctaaa gaggaatatc
    13141 atccaaagat attaaatgat actctacctg taaggtttga ttttaaaaat atagaagata
    13201 aaatagtctt atattctaaa gattcactac ctgttccact tactaaaaaa aatgatgtac
    13261 ttttatatga tggcaatatc tatttattat cacataaaaa agctcatgat tattcaaaaa
    13321 tatatcaaat attatcaaag actaaagagt taaaatttga taaagaggat tctaaggatg
    13381 ttttaggatt gctagttcct agattaaaat ctatatctca agaagttcat ttagatgata
    13441 acataaaaaa taatataaca aaagatttta aatctgagtt ctactttgat atgattgatg
    13501 gcaacatatg ttgtgatgta aagtatatat atgatgatga agataaaaaa tttgtgctcc
    13561 ccaacataca aaaggaagct actattgaaa ataaacttac ttcttcacaa tttacaaagg
    13621 aaaataatca ttatgtgttt aaaggaactg accatgacct atttacattt ttagatttac
    13681 aattagaatc tctaaaggaa tttggagata tatattattc tgagaagttt aaattgaaaa
    13741 aaatatataa cgcttcatca attcaagctt caatcaatca gactaaccag aattacctag
    13801 aatttaattt taatatatct gatataaatc caaaagagta caaggatatt ttgaaagctt
    13861 ttcaagaaaa acgtactttt tataaactta aagatggaaa ctttatagat ttatcagaaa
    13921 gagaaacaaa agattttttt gaattagttg aaaatctaga tataatggat aaatcaaaaa
    13981 ataataaaat tcataaaaat aaagctctat atataaatga tgtaatacgt agtaaaaatt
    14041 taaagtttat agatggtaaa aaagaactag acagtatatg tgaaaaattt agaaattttg
    14101 atagcataaa tttagaaatt cccgcaaatt taaatgcaaa ccttcgtgac tatcaaataa
    14161 atggcctcaa ttggtttaaa gttttagatt attataaatt tggtggtata ttagctgatg
    14221 aaatgggtct tggtaaaaca atacagacta ttgcattttt attatcatta tctaataaaa
    14281 aaagtcttat tgttacaccg acatcactca tttataattg gaaaaatgaa tttgaaaagt
    14341 ttgctccaga tataaaagtt ttattgatac atggaaataa aagagataga gaaaaatgtt
    14401 ttatggaatt agaaaatttt gatgtaatac ttacaacata tggtactctt agaaatgatt
    14461 tagacaaata tagtgaaata aagtttgatt attgtatatt agacgaagct caaaatataa
    14521 aaaatccagt ggctttagta actgagtctg taaaaagtat aaatgcagaa aataaatttg
    14581 cactcactgg tacacctatg gaaaataatc tactagaatt atggtctata tttgatttta
    14641 taatgcctgg ctatctatat agtaaagcaa agtttcagga attgtttata aataaagaag
    14701 ataatgttaa aaacctgaaa aaattgataa aaccttttat tcttagaaga agtaaaaaac
    14761 aagttatgaa agaacttcca gataagattg aaaaaaactt ttttgttgaa ttgaataaag
    14821 agcagaaaaa gatatatagt gtatattcaa aagatataca agataaaatg aaagataaaa
    14881 atctaaagaa agataaaata gttatattct cttatttaac taaactaaga caactttgtt
    14941 tagacccaag tatagtagta aaagattaca ataaaaaaag ttcaaaaatt gaaacatgtt
    15001 tagaaatact tagggatagt ataaacgaaa accacaaaat attattgttt tcacaattta
    15061 cttctgtgct taagaacata agtaaggaac ttgataagta taaaattaaa taccattata
    15121 tagatggtaa aactaatgcc aaagaaagac ttgaacttgt agatgagttc aataatagta
    15181 tggataaaaa agttttttta atatctttaa aagcaggcgg aactggttta aatcttacat
    15241 ctgctgacat ggtaattcac tttgacccat ggtggaatcc atcagtagaa aatcaagcta
    15301 gtgatagagc tcatagattt ggtcaaaaaa attcagtaca agttataaaa ttgattgcta
    15361 aagggactat agaagaaaaa ataataaaat tacaagaaag taaaaaagag cttataaatc
    15421 aatttataaa tggagaactt tctaatgaag gtgtattaaa aagtctatct gatgaggaga
    15481 taataaactt atttaactaa attatttaat cctacagctt aactatatga atttgttcta
    15541 acatttatat agttaagcta aaatatctga ataaataaaa tttaatattg ttatttaatc
    15601 ccaactttac aatctaatga tatctctttc tatacatatt gcatatacaa atgtaataac
    15661 ttgactatta gaatattttg ttttaacaat cctatttcat cataataatg aagtatatgt
    15721 acactcactt ttgtaagttt tgcaatccat tttacagttt tcattattgt aaattttggt
    15781 taaattattc ctctaaaaat ttctttgcta catttgaaaa atctaatcca ttcgataaaa
    15841 ttgcaggatg accaccttta tcaaacaaat acattttacc atttttaatc ttttcaagaa
    15901 gtaatgaaaa agtattttca aaaaaatcac tagatattag agatacaaac tcatcttctc
    15961 tacttcctgt taaaagcacc tctggttgca tagtttctag aggcttatga aaaaactttc
    16021 ctatagtttt ataatgttca aaaatagctt ctgtatcatt atctacaact ttttcccagt
    16081 tttctccttg attataaata taaaaagctt ttgcaccatc atcttgctta gatgcttcac
    16141 gctctatttt cacattttgt acaaatgact ctagaggaac ttctccttca aaactatctg
    16201 caattacctt attcactaaa tcaggtctct caagtgcaac atttaatgca acaagagctc
    16261 ctccactact accaattatg tttacttttt tatagttcat tgcttctaaa aaacatatca
    16321 cttgcatagc ctcatcaaac cataagtcag cagaaaattt atcgaccatt tgagattttc
    16381 catgacctac aaaatcaatt aatattactt tatattcatc tttataaaaa ttgattatat
    16441 cattaaacat cttggaagag gctgtatttc catgtaaaaa aagtaatggt gtaccatccc
    16501 caatttcatt atagtagcac tgtttgtcgt tatagttaaa atatgccata tatttccccc
    16561 ctttaatatc cctataggta tatatttatt acttatattt aaaatgacta ttcccttagc
    16621 cacttaaaaa tagtataact tagaatgtag ataaaatcaa cattccttgc taatttatct
    16681 attgtttgtc taatttatta taatttaact tctgtaaaat ttcttttttg tacgaaaata
    16741 ttccttccat ttacaacaat cattcaaatg aagtataaca tgacgagtag gaggcataag
    16801 caaaattcca gcaatatctt tattccccca aaagtccaac aaccaaatag aatcttcatg
    16861 ctttattact cctccagtca ccaaaatcct ttctaagtca attggagaga cctttcgttt
    16921 catatcttca gctttcaaat ccttaacaac ttctattgtg ttttttgcaa cttcatttcg
    16981 ataacaaagc aattcatcaa tattgactaa attatcgtat atctcttttc actttaacca
    17041 tttttttctt tataggtatc tccttatatt tttttaatat caaatctccc ttataattta
    17101 atatatctac atatgaacat aaatcttgaa cttcaattac acctatatca tcattagtta
    17161 taccttttat attactaaat gcaccaacta tatctaaaac tcttattttc ttcttcttac
    17221 cagcatttaa atataattta acaacttcac tttctatatt tagttttata tctttacttt
    17281 tgtaagaatt tttatcaact ttttttctct ttgatacttt ttttaggatt ccttttgaat
    17341 attcttcaaa caaaactttg ccatgtgtaa ttctactttt ttctatatct tttaattcat
    17401 ttattttata tcctatatac gtttctatat tctctatata tttttcatcc ttttcagtta
    17461 ctatagtaat tgcctttcca ctatttcctt ttctcccagt tctacctatt ctatgtatat
    17521 agttttcttt atcttgtggc acatcataat ttattactaa agaaatatca tcaatatgta
    17581 ttcctctgga cgctacatca ctactaacta atatattaaa tttttggttt ttaaaatcct
    17641 taattacaaa aattcttctt tcttgtgata aatctgcatg taactctctt attaaaaaac
    17701 cctctttact catcttgtta tataatttag atactttctc tttggtatta caaaatatta
    17761 tcacactctt tggattttcg ctgtatatta attcttttaa aaggatgtac ttctctcttc
    17821 catttgcctt gattattttg tcatctattt gtcttgtttt ttgattagta tcgctctcat
    17881 tttcttctat atttataaca ctgtaatcta gcatatattt ttcacatata tattttatct
    17941 cttcatctat tgtagctgaa aaaagactta ctattttctc cttaggtaaa tttaataata
    18001 ttttttccat ttgttcaaca aaacctttat taaacatttt atctgcctca tctattacta
    18061 aatactttac attttccaac ttaatagaac ctctatttat atggtcaagt attctgcctg
    18121 gagtagctac aacaatatga accctttgtt tcaattcagc tatttggtct tttatagatt
    18181 gtttgccaaa tatagcacta catctcacct tctttagtct ccctatatct gatatctcat
    18241 cttttacttg caaggctaat tctcttgttg gaaccactat aagagcttga atattattgt
    18301 aatctacatt tatattctcg cataaaggta ttgcaaaact tgctgtcttt ccacttcctg
    18361 tttttgacct aactactaag ttttgacctt ttaataattt tggaataact tctctttgta
    18421 ctctagaagg tatattatat cctaaactct tcaatgactt aagtatcttt tcatttaact
    18481 taaatttttc aaatgtatat ttcatatatt accaccttat tatatttatc ttatctatat
    18541 atttttctac attatattat tataatacaa ggttctacta tttttatatt atttattaga
    18601 ctgaagataa aagtatatac tctataaacc tatagtttag tagcaaatta attaaatttt
    18661 tatatttaaa tacctcctta aatatactga taaatcaata ttttcaagga ggtatctctt
    18721 gtcaataatc aaactaatct atcttttgaa agtttaaatt atactaatac attgtatttt
    18781 tatttatatc taatattaaa aggtattaat caatacctaa atcaccataa tctcacatat
    18841 ttatatcttt attcaatata ccttctgtaa tcaatcgtgc tatataatca taacctgatt
    18901 catttgcttt gttacaatca ttcacattat cacagaaaaa actttcaatc tgtattgcaa
    18961 ctggatttgt atttctcaat atatataaat tttctctttt tttagctcct ctattttcaa
    19021 ataattctcc aagtctatta gatactcttt tagcatattc taatccttca gtgccatagt
    19081 aaagcacttc tgtaccacat tgtgttccat cagctttatt taaatgtagc tctactaata
    19141 aatcgtattt accactgttt acttttggaa ctctatagaa aaattcttct ctttctgaca
    19201 taaagtgcct ttctggacaa attattacat ctactgtatt accttgactt ataagcatct
    19261 cagctaacat aggagctaat tttttattat aattgtattc atcaactata ccatttacac
    19321 ctgtactttt accacctttt agtatactat gacctactgt tatgcatatt ttcatttact
    19381 tttttcacct tcttttaagc caatttttat ataatagtaa tataatttta aagttattac
    19441 taataataat atattaattg aagaaggtaa aagttctccg tgattatcat ttttttaaca
    19501 tatttgtctt attttatcat aaatctatat tttttaaata ttatactata cttattattt
    19561 ataattgttt aattaatcat ttatatatat gataaatata tcaatatcta ttttccttta
    19621 accaaatgct attttaataa gtttaagttt tagctttaaa atacttaata atacaataaa
    19681 agtgatgtat ttacaaataa ttcttctgga tttaattcat ttattgatgt atgttcaatt
    19741 tttatattta tatttttcat atcttcataa ataattccaa ctggagaaca aatgaaacaa
    19801 gaaaatccaa aagtttttgt tatttttttt accttattat caccaaaact tatttgcttt
    19861 ttacaatcgt ttatattttc attgttattt tgtatatcat ttttataata cttatctttt
    19921 ttaatgtcat tagacttatt tactgtttta aaattactcc tatttttaca actatagtct
    19981 atttcaactt ctatataccc aaaaactatc aagttacata atttcaaatt actacctttg
    20041 tttataagat ttttcatagg tttaacatca aatattttac tatttacatt tacttttaat
    20101 atatctctta gatttttagg agcttttaaa gtattactta tattcaattg tttgaataca
    20161 attttattta cattagtata aactctatta ctttctattg atattcttgt tgggaattta
    20221 tctagattac acttataata atagtatata ttagcactat tctttataat cctatgtgca
    20281 gaatttggta aaactacact cttaaaagat attatttttt tacaacttgc atctattctc
    20341 cctatgtaaa aaccttgctg tgggtctaat ccacgtttta taatgccatt aacttttacc
    20401 gtattattta taaactctat atatttagaa atataatctt tgaaaaatat atttccagtt
    20461 ttgtacctac ttgtatttgt aacaataata gtgtaggtca aaatctcaaa tacagatgca
    20521 cattttttat caacatattt ttctactttt ataatacttc catttgcatc atttgaaaat
    20581 acatcattaa aagtagtttt cgttattctc attgcatcca cctttctata atttaaagat
    20641 tactctatct tggaactatt ttcataactt ataattgcag taacactaat aaataaatta
    20701 tcatctaaaa tagatgaagt aatatcttgt ataagatact ttaaattaat tttatcttta
    20761 ttttctatct ttctaggcat ttgtataaat gtactaaagg gtatttttaa tttaaatgta
    20821 ttttttttac tagaatttct atttctttta ttgcctgcat aatctaatat tgcatcaata
    20881 tctatatcac caactaaaat caacttcttt cctgtaagtt gttcatcttc taatgatata
    20941 cattctatag tatcaacaag acttatattg ttaatattca tattgtaaac atatgtctct
    21001 aatgaattta ttacagtttt tttagaagct aatttaaaat ttttatatgt attaaattga
    21061 gataaataaa gagtcttttc tagtttttca catctttcaa ggttgtatat taccttatta
    21121 ttttcataca taatcgcatc aatctccctg tatctatata ttaatactat tcataaaaat
    21181 tatttcaaat taccattttt tattattcac tatctagttg ttatttactt ttaagaaaga
    21241 atcctttttt acttcattga tttcaattta caagtaaaag tctttctgaa aaaaaaatat
    21301 atatatctct attcactttt ttataagtaa ttataatttt tatagactaa cggtcagcct
    21361 taagggaaaa ggtgttcttt aagtgcagaa tttagataaa gtaaataatc cttttataaa
    21421 taattccaag ttttaactta aataatttga tttacagtat aaagtaaatt ttgaaagata
    21481 atgatatcga tttaataatt acaagtaaaa gcatgtcaaa ataaggctat gattttaaaa
    21541 atcatagcct tatcaataaa aatttaatca aagttacatt tcactttgaa tttcactgtc
    21601 tattaattga agattatact gttgaattaa ttctattaga cgagctgcat atatactatt
    21661 gccattttca tccttcgcag tactataacc tgccttttct aatgctttag cttgatatat
    21721 atacgttttt gcatcaaata caccattgtt tttatacctt ttatttgtgg ctaaaaattt
    21781 tgcatggtct tttatagatt catttttatc atcatatatt ctaaatttat catctataat
    21841 tgtatctaaa tgttctttcg tttctaaagt cactgacttg cctttccaat aagcgtcagc
    21901 ctttatacca aatagattgt tatattgttt tgccaaagtt gacttgcccc agctagattc
    21961 tattatagct tgtgcaatag ttatagatgg aagtatttta taatccttat aattttgtat
    22021 tgcttctgtt ttaatttcat caataaactt catatatttt gtatcagact tcaatctttc
    22081 tggttttata ccataatcct taagattgtc tatataatta tcaaccaatc ttttttgtct
    22141 attactaaat tctaactcat ccaaaatatc acttaactta tttattttat tatttttaga
    22201 aaaattctta acaaataaat ctgatacttc ttgtatctgt gaatccttaa cgttttttaa
    22261 gttattttta tttaacacag caacaatgga agctacatac ttccagttaa cttgtgcatt
    22321 atttttactt gctaaatcag catattttat atacttactg acatttatat catccttttt
    22381 tatctctcta gatgaatatt ccttcactaa tacacctatt aaaattgata ttatcagtat
    22441 tataacaatg aattttttat tcttcataaa tattgtcctc ccttatacta tctgctgaat
    22501 ataatatacc ttacttttgt gacatattct ttacgaaatt ttgaaaattt tatgaagatt
    22561 tatgaaatat atttaattaa aagcactact attgttataa tatatagttt tctatcacac
    22621 aaaatagtta aaataaataa ataattagat agcttatttt aactatttat ataataaaaa
    22681 tattattaaa tctatttatc aatttttata catttttcta atatatattt tttcccatat
    22741 acaataattt ttattggttt accttttaaa ttttctattt taattaaatc actacataca
    22801 tctacattaa taatattatc tctataattt atattgaaag aataattttt ccactctttt
    22861 ggcaacttag ggtcaaaaga tagagtttca ttccatgttc tcatgcctgc aaatccatgt
    22921 acaatggaaa gccatgaacc agacattgaa gttatgtgca gtccatcatt tgtatcatta
    22981 ttgtaattat ctaaatcaag cctagctgtt cttgaatata attcataagc cttttctagg
    23041 ttatctattt ctgaggcaat aatagaataa atactagaag ataaagaaga ttcatgtaca
    23101 gtatattttt catagaagtc aaaatttctc tttttttctt ctttagtaaa tctatttcca
    23161 aaatagtaaa ttccctgtaa tacatctgct tgttttataa aacacgacct aagaatctta
    23221 tcccaagacc aattttgatt aagaggaaaa ttttcttttg gtaaatcctt tacttctaca
    23281 aattctttat ctaaaaaatt atcattttga attattattt gtagttcctc atcatacagc
    23341 agatacattt tatttgatat tattttccaa aactcaactt cttccctagt cacattattt
    23401 ctgtcaatac attcattaca ctctttctct aatcgtaaga tattttctac agcataatct
    23461 aaacaccatt tagctatata attagtatac caattattat tgatattgtt ttcatactcg
    23521 tttggacctg ttactccatg tatcatatac aaatcttttt ttgtagacaa atgaactcta
    23581 cttgcccaaa atctagctat ttctattatt acatctattc ctttttcttt tatatattta
    23641 tagtcacctg tataatttgt atagttatat attgcatata ctatacttcc atttcggtgg
    23701 atttcctcaa atgttatctc ccattcatta tgacattcta ctccatcaaa tgttaccatt
    23761 ggatataaag cacctttaag acctagtttt tttgcattat cctttgcctg ttctaactga
    23821 ttgtacctat attctaaaag atttctagtt acctccttat ctgctacacc tagatatact
    23881 ggaacacaaa acgcttctgt atcccaataa gtagctcctc catacttttc accagtaaat
    23941 ccttttggac ctatatttaa tcgcgaatct tctccataat atgttgaaaa tagttggaat
    24001 aaattatatc ttataccttg ttggctactt ttatctccat ttatttttac atctgcactt
    24061 tcccatcttt tattccataa ctttatatgt tcatttaata tatcatcata acttttagat
    24121 agttctctat ttaaaatgtc ttcacatttt cttaatagct cactttcctt ataatctcta
    24181 gttgaggtta tagatacata tttttctata cttgcaacct cattttttaa aacatccatt
    24241 gttatcgaat tttcacagta attataatca ctctcatgag atacagcttc tacatcacca
    24301 tttgtcttaa tatacatcat tgaacccaca gtgaatactg gtgtattaaa tgcattctcc
    24361 tttgttcgag aaagaatact ccctttatat tctaaagaac ttgaagaaat tccattccaa
    24421 aacttttcct catagtttga atcttcattt tttatatttg aatctaaata tggtacaaat
    24481 tttattttag tattagaatt taatgatttt atactgtatt taattactgc aagttcctta
    24541 acagatatac ttaaaaacct agtaacccta acttcaaatt ttttatcatt tatatttaca
    24601 ataaacctac gatttaaaat accatttttc atatctaatt ctctatgaaa atcttctact
    24661 ttacattttg ctaaatctaa ttctacatca tctatgaaaa ttcttacacc tatatagttg
    24721 actgaattag gaattttacc aaagtattct ggatacccat ttttccacca accaacacga
    24781 gttttatctg gaaaccatac tccagctata taagacccct tgtgtgtatc tccactataa
    24841 ttttcctcat agtttcctct catacccata tatccattgc ctatactcat tatggactct
    24901 gctaatctat tttctttgat ttcaatctta tcttgtataa tcttccactc atctacatta
    24961 ctaaataaac atttcatata attctcctaa ctatatactc attatacttt tcttgtatat
    25021 attcaaactt taattgattt gttgaatcta caactaaatt tgcttttttt aagttttcat
    25081 aatttccaac accaacagaa aacatattag ctgagtttat agcgtctata cctgcacttg
    25141 catcctctat ccctatgcaa ttttgtggat ttacattaag ccctttcgct gacataagaa
    25201 atatctctgg atgtggttta ttgtttttac attttcctgc atcagctata aaatcaaatt
    25261 tgtcacttat acccaaatga tttaatacat ttattgcatt cttacttgca gaagatagac
    25321 ctatctttat attattagat tttacatcaa ttaacaagct ttctatgcct ggcaaaatat
    25381 catttgaagt tatctcatct atcaatgata catagtaatt atttttttct tcagccattc
    25441 tcactttttc ttcttctgaa aatgaatatt tcttatttcc aaattctaat atcctgtcca
    25501 atgactccat tctacttatg ccttttaaag attcattaaa ttttgtgtct atatctatcc
    25561 caactttatg agctagtttt ctccaagcca tatagtgata atatgcagta tctgtaatta
    25621 caccatctaa atcaaatata aatgcttcaa tcattcttat ttcctctttc tacaagaaat
    25681 ttttagacta ataatcaatt tattttctat cttgtattta agatattatt tataaaatta
    25741 tctactctaa ctcagtcttt aatttaaaat ctactttcaa ttcactcact tgtagaccat
    25801 attttttatc gtttataaag acttgtttaa actgtataat aatttaacat gaaatctaaa
    25861 ataaatactt ttttgtatta ttttaaaata atgaacttgt atttctctaa cattagttct
    25921 tcttttaaat caactatttc ttcttcatat aaatctttta cttttttatt tgctaaataa
    25981 tctggtaacg tcaaaattat atttttagat gaattattta ttattataga aatagattct
    26041 tttttcaata taatcgttcc attttcctta tctgctaaca cccattcatt tgatatagac
    26101 ctaagttctt tattttcttt acgaagtcta ataatcttct tcataaaatc aaatatatcc
    26161 ttattccatt tttcttcatt ccattccata cattttcttt gaccttcaga tattgaactt
    26221 aattctcctt ccattccgat ttcatcacca tagtatatac aaggacatcc tgtttgtaca
    26281 aacataaaca tatatgcaag tttaaatcta tcaatatttc cattagcata agtcaatatt
    26341 cttgtagtat cgtgacttcc aagtaaatta aaaataactt cactaacttg tattggataa
    26401 cttaccatta catcatttat catatactta aattcttcgg cgtttatctc atctgtacaa
    26461 aagaattttt tcatggcttc tgacatcaag taattcataa ctgaatcaaa ttggtcaccc
    26521 attaaccatg gtaaactacc atgccaaatt tctcccaata tataaatgtc tggttttact
    26581 tttttgactt cttctctaaa ctttctccaa aaaacatggt ctacttcatt tgaaacatct
    26641 aatctccacg catctatatc aaattcctgt atccagtact tgcctacatc caataaatat
    26701 tttattactt ctgaattttc tgtattaagt ttaggcatat atttttcaca cccaaaggtt
    26761 tcataaggta tatttttttc atctatttgt tctattggag tatcaatttt actcatatct
    26821 tttatataaa accagttttt atatcgagaa ttttccttat tttgaactac atcttgccac
    26881 tgtttagaat aatatcctat atgattaaaa acagcatcca acattatttt tatatttctc
    26941 ttatgcgctt cttctattag tttttttaaa gtctctttat ctcctaatgt agggtctacc
    27001 tccatataat ctaccgtatc atatctatga ttggtttttc caattgtaat agggcaaaag
    27061 taaattccgt ttattcccaa atcactcaaa taatctaagt gttctatcac accttgtaaa
    27121 tctccacctg taaaattatc tctggttgga atagctcccc atttatctac tccttctggg
    27181 tttatagacg ggtcaccatt tgcaaatcta tctggaaata tctgatacca aacagtatct
    27241 ttcacccaat ttggcacttt aggtatatct atataattta aatatggata tccaaaaaaa
    27301 ttacctattt cacataattc cttttcatca ttttcatttc ccaactctat tattcttttt
    27361 tctgtaaata aaagtttttc ctcttgacct tctattataa agccatatcg tgagcgttta
    27421 tttaaaggct tacacttagc tatccaatag tcaaataatt cagtttctac ttctttactc
    27481 attactatat ttgttccacc agaccaacca actccagatg catttaaatt tcctcctcct
    27541 gcacctcctt tatcccacac atattggtct cctattcgta aaattactct tttcgcttca
    27601 ttttttttag ttctaagtct tatatgtagt gtttctctat cataaccata ggcataattg
    27661 ctcattggta tatgtaatat agcttctttt gttaaactat tttgaattgt tcctttgatt
    27721 tgattactta aagttatatt ttctttattt ttattagaca ttttaaaatt tccctccttt
    27781 ataa
//
LOCUS       DAYAOX010000027        27705 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.027, whole genome shotgun sequence.
ACCESSION   DAYAOX010000027 DAYAOX010000000
VERSION     DAYAOX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.027"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            103..1650
                     /locus_tag="ACGI57_000958"
     CDS             103..1650
                     /locus_tag="ACGI57_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFG9716044.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            1653..2051
                     /locus_tag="ACGI57_000959"
     CDS             1653..2051
                     /locus_tag="ACGI57_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFG9716045.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            2091..3824
                     /locus_tag="ACGI57_000960"
     CDS             2091..3824
                     /locus_tag="ACGI57_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFG9716046.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            4056..5267
                     /locus_tag="ACGI57_000961"
     CDS             4056..5267
                     /locus_tag="ACGI57_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFG9716047.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            5460..5720
                     /locus_tag="ACGI57_000962"
     CDS             5460..5720
                     /locus_tag="ACGI57_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFG9716048.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            6074..7786
                     /gene="ptsP"
                     /locus_tag="ACGI57_000963"
     CDS             6074..7786
                     /gene="ptsP"
                     /locus_tag="ACGI57_000963"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFG9716049.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            complement(8578..9243)
                     /locus_tag="ACGI57_000964"
     CDS             complement(8578..9243)
                     /locus_tag="ACGI57_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716050.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            9587..11017
                     /locus_tag="ACGI57_000965"
     CDS             9587..11017
                     /locus_tag="ACGI57_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFG9716051.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            11385..11963
                     /locus_tag="ACGI57_000966"
     CDS             11385..11963
                     /locus_tag="ACGI57_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="HFG9716052.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITISSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            12017..12139
                     /locus_tag="ACGI57_000967"
     CDS             12017..12139
                     /locus_tag="ACGI57_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HFG9716053.1"
                     /translation="MKFASSLALSTAILSANSTCPWIIHQPKVPKEISNLKKTN"
     gene            12435..13814
                     /gene="scfB"
                     /locus_tag="ACGI57_000968"
     CDS             12435..13814
                     /gene="scfB"
                     /locus_tag="ACGI57_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HFG9716054.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            13843..14340
                     /locus_tag="ACGI57_000969"
     CDS             13843..14340
                     /locus_tag="ACGI57_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFG9716055.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            14638..16302
                     /locus_tag="ACGI57_000970"
     CDS             14638..16302
                     /locus_tag="ACGI57_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HFG9716056.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEAIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            16315..18765
                     /gene="recJ"
                     /locus_tag="ACGI57_000971"
     CDS             16315..18765
                     /gene="recJ"
                     /locus_tag="ACGI57_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFG9716057.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            18778..19290
                     /locus_tag="ACGI57_000972"
     CDS             18778..19290
                     /locus_tag="ACGI57_000972"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFG9716058.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            19364..21571
                     /locus_tag="ACGI57_000973"
     CDS             19364..21571
                     /locus_tag="ACGI57_000973"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFG9716059.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            21596..22045
                     /gene="dtd"
                     /locus_tag="ACGI57_000974"
     CDS             21596..22045
                     /gene="dtd"
                     /locus_tag="ACGI57_000974"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFG9716060.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            22060..22674
                     /locus_tag="ACGI57_000975"
     CDS             22060..22674
                     /locus_tag="ACGI57_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9716061.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            22765..24321
                     /locus_tag="ACGI57_000976"
     CDS             22765..24321
                     /locus_tag="ACGI57_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HFG9716062.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            24311..25573
                     /gene="hisS"
                     /locus_tag="ACGI57_000977"
     CDS             24311..25573
                     /gene="hisS"
                     /locus_tag="ACGI57_000977"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFG9716063.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            25647..27434
                     /gene="aspS"
                     /locus_tag="ACGI57_000978"
     CDS             25647..27434
                     /gene="aspS"
                     /locus_tag="ACGI57_000978"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFG9716064.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT        11295 a         2691 c         4950 g         8769 t
ORIGIN      
        1 tatatgtgat tttaacttgc attgatttgc tatcaattgt gctttataat tagtatatat
       61 aaagtttaac tgtaagataa tttaaaaaat aggggaattt atatgaataa aaatagcatg
      121 acaagaattt acagtttaaa taaaataatt tttattataa ttggaatttt aggaatagtt
      181 ttactcacaa gacatattga ggtagatatt aaagttttaa ttttaatttt attaaccttg
      241 tttagtataa atgtatttat ttcatatctt aaaattaagt tgtatgagga taaaattaat
      301 ttaggattga taaataattc agataagttt ataaatatta ctaaagatga gaacttccta
      361 aaacatgttc aaaattatat tatcagtaat gaaaaagaaa actgtgtaat ggcttgtttt
      421 gatttatgta gactcaaact tataaatgat atatatggtt atgagctagg ggatgaagtg
      481 ttaaataata ttttaagtaa cttaaaaaat tattttggaa aagaagctat ttatggaaag
      541 ttaaaaagtg atgtttttgt attaataata aagcttgaaa atagggaaca gaagattcct
      601 tatttagtaa atcttattaa gaataaaatt gtgatactaa gtcagattga cagttttaaa
      661 atggatataa atattgaggc ttctattgga atttataaat ttaacaataa tgaaattgat
      721 ataaaaaaag ctatagataa tgctgatatg gcaagactta aatcaaaagg actaaagcac
      781 atagagtatg tagaatttga taatgctatg gaagaagaaa tacaatcaat tatgaaaata
      841 gagagagatt tattcttggc tattaaaaat aaagagtttg taattcacta tcaacctaaa
      901 gttgattcaa gtacaggtaa cattataggt tcagaggcat tgataagatg gttgcaccca
      961 tcattaggca tggttggacc aaataagttt atacctatag ctgaaaaaaa tggtctgata
     1021 aacaatattg gtagatggct aattcaagaa gtttttatta ccattaacaa gtggatttca
     1081 gaaggtataa atgtcttacc agtctcaata aatctttcta gagtagaact atataaaaat
     1141 gatttagttg atttttttaa actgatgttt aatacatata atattccaaa agaacttata
     1201 gaattagaaa ttacagaaac gacagcacta agagatgtaa aatttataag tgaaaggctt
     1261 tatgagatta aatctttagg tatgaatata tcattagatg attttggagt aggtaattca
     1321 aattttatca accttaaggg gataccacta gatattataa aaattgatag gtcacttata
     1381 ttggatatag ttacaaatac aaaaacaaag tttatggtga aagcaattgt taacctttca
     1441 catgatttaa acgtaactgt tatatgtgaa ggggtagagg atatgcatca ggtcaaagtc
     1501 ttaagtgaac tgggatgtaa tttcatacaa ggttatgtat tttttaaacc tttggatgag
     1561 atgaattata aaaaacttct tactgacgga agttatataa atctgaaaga tactctttta
     1621 gataaatgca tgacagttga ggaggagtaa ggatgatttc agaagatgta gattattcaa
     1681 taagaatcat aagagctata tttaataatg gagaagataa accaatagat gctaaaatga
     1741 tattaaaaag tgaagatata ccaatactaa cactatacag actgcttaga aagctttgtg
     1801 caaacaatat tcttactttt aacaaagaag atggcaagac atacatgtta aatgtgtcat
     1861 tggatacaat aagtctaaga actattgtag aagtaattga tggaaaaata tatataaata
     1921 aatgtacgag atatgtatct aattgcagta attgcttaaa aggttgtaaa gtaaatcgtg
     1981 aattgctaga aatagaaaaa gaaatttata aacttttatc gagaaaatct ataagacaaa
     2041 tactagaata aaaaattttc agctatgata ataattggag gttttcggtt atggatgata
     2101 tatctcaaga taattttcta ctgtctaaag agtatgagaa tagcttagat gtagatacta
     2161 aaaaagcaag tggaatatat tatactccta aaataattgt agattatatt gtaaaaaaaa
     2221 ctttaaaaaa tcacgatata attaaaaatc catatccacg aatacttgat atttcctgtg
     2281 ggtgtggaaa ttttctttta gaagtatatg atattttata tgacttattt gaggaaaata
     2341 tatatgaatt gaaaaagaag tatgatgaaa attattggac tgttgataac atacaccgtc
     2401 atatattaaa ctattgtata tatggtgctg atattgatga aaaagctata agtatattaa
     2461 aagatagctt aacaaataaa aaggtagtaa atgatttaga tgaatctgat ataaagatta
     2521 atctattttg ttgtgatagt cttaaaaaga aatggcgata taagtttgat tacatagtgg
     2581 gtaatccacc ctatattggt cataaaaaac ttgaaaagaa atataaaaaa tttctcctag
     2641 aaaaatatag tgaagtttat aaggataaag ctgacttata tttttgtttt tataaaaaga
     2701 ttatagatat tttaaagcaa ggagggattg gcagtgttat aactccaaga tattttttag
     2761 aaagtctatc aggtaaagat ttaagagaat atattaaatc aaatgtcaat gttcaagaaa
     2821 tagtggattt tttaggtgca aatatattta aaaatatagg ggtatctagt tgtattctaa
     2881 cttttgataa gaaaaaaaca aaagagacat atatagatgt atttaaaatc aaaaatgagg
     2941 atatatgtat aaataaattt gaaacattgg aagaacttct aaagagtagc aaatttgaac
     3001 attttaatat aaatcagaga ttgctatctg acgaatggat acttgtaaat aaagatgatg
     3061 aaacttttta taataagata caagaaaaat gtaaatatag cttggaagat atagcaataa
     3121 gttttcaagg aataattaca gggtgtgata aagcgtttat tttaagtaaa gatgatgtaa
     3181 agttgaacct tgtagatgat aaatttttaa aatgttggat aaaaagtaag aatataaata
     3241 aatatatagt agataaaagt gaatataggc taatatactc aaatgatata gataatgaga
     3301 atacaaataa aaggatacta gatgaaatta tagggctata taaaacaaaa ttagaaaaca
     3361 gaagagaatg taaaagtgga ataaggaaat ggtatgaact tcaatggggt agagaaaagc
     3421 ttttctttga aagaaaaaag ataatgtatc cttataaatc aaatgaaaat agatttgcta
     3481 tagattatga taataatttt tcaagtgcag atgtatattc tttttttata aaagaagagt
     3541 atttagataa attttcatat gaatatctag ttggaatttt aaattccagt gtttatgata
     3601 aatactttaa aataactgct aaaaaaatga gtaaaaatat atatgattac tatcctaata
     3661 aggttatgaa gataaggatt tttagagata ataattatga agagatagag aatttatcaa
     3721 agcaaataat atcaatttta ttaaataagt ctatagataa aggaaaagta gaaaaattgc
     3781 agataaaaat ggataacttg ataatggatt ctttaggtat ataagcagtt gtaagtatta
     3841 aaaaattata atcttttaat tgtttattta aatttagaaa taaaaagtta gccatataaa
     3901 aaatattaaa aatatatact tttcaatatt tttttgataa aataagaatg aatttgtgca
     3961 attttatttt tctccaataa ctaaaaatag ttttttgaat taatatttgc ttaagaataa
     4021 aaatcagaaa cgtttttgat atagtaggtg ataatatgaa aggattagtt ttagaaggtg
     4081 gaggaacaaa aggagcctac caaataggtg catataaagc tttaagagag cttggtatag
     4141 agtttcaagg agtaactggc acatcaattg gtgctttaaa tggagcatat atagttcaaa
     4201 atgatattga agtaatggaa gatatttggt taaattatga ctatactcat tttatgaata
     4261 ttgatagaga aacctatgag aggtttaaaa atgtagattt cactactaaa aatctcaatg
     4321 cagtgtttga gcttataaat aaagctagaa aaaatgaagg tatagatata agcccattaa
     4381 gacaactaat ggagacaaca ttaaatgaag acataataag gaaatcaaaa aaagactttg
     4441 gacttgttac ggtctggtgg gataaaaaaa ttaatccata tccattgtat atcgaagata
     4501 tacctaaagg aagattgatt gattatttaa ttgctagttc cagtttgcct atttttcaaa
     4561 cgaacatgat ggatgataag ttgtatcttg atggaatgtt ttgtgataac atgccaatag
     4621 gactcttgga agacaaaggt tatgaagatg ttgttgtaat taggttgatt gatgatttta
     4681 ttgggaaaat gaatctaaat cgacatcaaa atataaatat aaaaactata ataccatcag
     4741 aatatctagg aggttctctt aataaagata gagataatgt agaaaaaaac atccgactag
     4801 gttacttaga tactatgaaa gcatatgaaa gatgcactgg aattaagtat tattttgatg
     4861 tagagtataa atataatgaa gaatattgtt ttgataaatt tagaagtatg agcaatgaaa
     4921 caatacaata tatctgtaat ttattgaata taaaaagaga tgcttcactt agaaccgtta
     4981 tggaaagtgt aataccaaag cttggtgagg tgcttggatt gccaaaggat ttttcatata
     5041 aagatttgtt ttactgtata tatgaaaaga aacttgaaga aaataatata aacaggataa
     5101 aattatatga ttttaacaag gttatagatg ttgtaaatac aaatattgat tctaatagaa
     5161 atttagcttc taattttgaa gttagaccta aaattagtat taaaaagaac aaaaacacaa
     5221 aattattgac aaattgtatt ataaaagaca taaaaaatca aagttaaata aaaaacaaaa
     5281 aaaaatttgt aattatactc gaaaaaagtg tatattagta gtataatatt tttgtagcac
     5341 tagtgggaac aaaaaatgac cataggacaa aaaagtacca cttagtacca ttaaagtaaa
     5401 aaacaggaaa acaattttaa aaaatataaa atgtaaagtt tataggagga tttaaaaaaa
     5461 tggaaaaagt agttagtatc aaaaatgcaa gtggattaca tgctagacca gcaggaatgt
     5521 ttgttaagaa ggcagctgaa tttaaatcaa cagtagaggt tgtagcaaaa gacaaaactg
     5581 taaatgcaaa atcaataatg ggaataatga gtttaggttt agctcaagga gaagaattaa
     5641 agttagttgt aaatggagaa gatgaagaag tagctttagc tgcaatggtt gaattagttg
     5701 aaagtggatt tggagaatag atataataat tttttaaaaa tttgatattt agaatctaat
     5761 attacttaaa gtaaagtaac agagaagtat taaacatata cagaccaaca tttgtagata
     5821 tttaatttct ttgttacttt catgtttatt taatcagaag attaagacta ttgttaattg
     5881 ggtataaaaa tataaatatt ttagaaaatg aaataagata tttttaatca taaattcaga
     5941 gtaaaaatac agtaacaaat agtttataaa attttataaa aaattataag taaataaaac
     6001 tataaaaatg taaattattt atataaattt ataaagttta tgtgctagat gatatttttt
     6061 aggaggtagg gttatggctt acaaaggaat aggtgcttct cctggtgttg ctttagggaa
     6121 agcgttagta gttgaacata gtgaattagt aattgaaaaa aaatctatag ataatgttga
     6181 agctgaaatt gctaaattag aagatgcagt tgcagtttct aaagaagaat tagtaaaagt
     6241 taaggaaaag gcttctgaag aattaggagc agaggaagca gaaatatttg aagcacattt
     6301 attagtttta gaagacccag agttaattgg ttctgcaata gataaaatta aaactgaaag
     6361 tgtaaatgca gagtatgctt taaatgaaat aaaagagatg tttgttagca tgtttgagtc
     6421 tatggacaat gagtacatga aagaaagagc ggcagatata aaagatgtta caaatagaat
     6481 acttagacat atattaggaa ttaaagttgt tgatttatct gctttaagcg aagaagttgt
     6541 attaatagca catgatttaa ctccatcaga cacagcaact atgaataaaa aaatggtttt
     6601 aggattttta actgatatag gtggaagaac ttctcatact gctatcatgt ctagaacttt
     6661 agaaatagct gcaatcgttg gattaaatga tataacttca aaagttaagg atggagattt
     6721 tgtggttttc aatggagaca caggtgaagt aatcgtgaat cctgatgaag agactataaa
     6781 taaatatact gaattaaaag ctaagtatga agatgagaga aaagcattac agctattaaa
     6841 aggaaaacca tctgtaactt tagacggaaa acatgtggaa ttagctggta acataggaac
     6901 acctaacgat atagaaggtc taataaagaa tgatgctgaa ggtgttggtc tttatagaac
     6961 agaattttta tacatggata gagattcttt cccaacagaa gaaatacaat atgaagcata
     7021 taaagctgta ttagaaggaa tggatggaaa gcctatagtt ataagaacat tggatatagg
     7081 tggagataaa gaattaagtt atttaagtat ggaacctgaa atgaatccat tcttaggtta
     7141 cagagcaata agactttgcc tagatagaaa agatatattt aagactcaat tgagagcttt
     7201 atatagagct agtgttcatg gaagattaag aatcatgttc ccaatgattt catctttaga
     7261 agagttgtta caagctaaag aagttgtaaa agaagtttta gcagaacttg attctgaagg
     7321 tgtggcttat gctaaagatg tagaaattgg tatgatgata gaagttccat ctgctgctgt
     7381 gatatctgat gtattggcta aacacgtaga tttcttctct ataggaacta atgatttaat
     7441 tcaatacact tgtgcagttg atagaatgaa tcaaaagata agctatcttt acaatcaatt
     7501 taatccagct gttttaagac ttataaagac tgttatagac aatgcacata aagaaggcaa
     7561 atgggcaggt atgtgtggag aatctgctgg agaccaaaaa atgataccaa tacttttagg
     7621 tatgggatta gacgagttct ctatgtctcc aatatcaata cttcctgcta gaaagttaat
     7681 aacttcagtt aaagagtcag atatgaaaaa attagctgat gatgtactta atatgggtac
     7741 tgctgaagaa ataaaaagct atatagaaaa gacttttaat atataattaa tgttatatag
     7801 ttactaaaat tcatttttct aaataacaaa attatatttt acgaattaaa aaggagatgt
     7861 ttcaaaataa aaatttgaag catctctttt ttataaaaga ttactaagaa ttacttaatc
     7921 tttgtaatag tcattttgtg aaaaagcaaa actgcattgt ggctatagta tatatggatg
     7981 ttattatcaa taaaagtact attaaaatga agattcattc tcaaatgata tcaaaagtag
     8041 ttgtgatgga gtaagataat attttgtgat ttaatagtaa aaattaaaaa aatatggtat
     8101 tgaaaaataa ttaaaagtat ttaataatag aattagggaa gaataagttt ataagtaaaa
     8161 ataattgatt actagttaat aaaataaatg tgatatccaa ataaatccta aatctaattt
     8221 gagttttaat atagatagtc taaagtttga ttattaaaga tttaattgta agttgttaca
     8281 gttgatgata ttgattagag attaaagttt agagtagaaa gttaccttaa atttaaaaat
     8341 gcaagataac ccaatctaaa aattataatc actcaatagg tttaaggatt tatttgttta
     8401 ttataaatag gagggtgtct caaaatgaac tttttagttt atgaggcact ctcttttata
     8461 tagaattaaa tatattttat tatttcaaca atgaaaaaga agcttatctc tattttgaga
     8521 cagcctcttt gtatactatt attaatttaa tcaataacat aatttaaaac tatattatta
     8581 gttaagattt cctgctttat tagtaaataa ttctttaata gcattgtttc tggaaactaa
     8641 atcgttatca gagttgtttg tttttttcat aatcactgaa taacgttttt tatttataaa
     8701 aaatattaaa atcttatcat catcaaaatt gtattcttta tatctgtcaa tagactcata
     8761 acctagattt agaatgtttt tgtcttttat actatctaca tctaaaatat atgatgggtc
     8821 agcctttagt aatatattaa ttaaacattt ttcttcacca gcacagaata actccatctt
     8881 acttgatata gatttagatt gctctgaagt tatttttgca ggaataaggt tgtcattgtc
     8941 atctcttaga agataaccat gtgtgtcttg ttgatatact aaattaacct ttttcttagg
     9001 aacactaatc attttatcta aatgtacata atgatgattt tcaacaaaac cttcatttaa
     9061 tatttcaaca aagttattaa ttttacctag gcaaaaagat tctatagatt ctttacagaa
     9121 tttatttgta aatggtatta tgtttacgtt ttcctcgtct gtattccatg ctatagcata
     9181 atccaacgaa tatacttctt cctctatatc tgcaaaatta tattgtacta tgtttccttt
     9241 caaaattact cgacctccat cttataatat atttaatata ctttataaaa cttatattat
     9301 atttaaataa tatcataaat tgaataaata tgatatagta aataagaaaa aatttgaaaa
     9361 aaatcactat aaatgagtaa taaatataaa tagtgtatac taatgggatg gaaaatgtcc
     9421 actattaact ttattacccc acatatataa gataaaatta ttaatataaa atataatcta
     9481 tcgacaatga atagactttt aaataaactt aatataaaat taattaaata agtgcttaaa
     9541 aacgatttac tttaatgata gaatcatatt aaaggaggat aattttatgg atataaagaa
     9601 taaaattaaa tctatatttt taccagaaaa aaatgactat ttagatgatg aagaatttga
     9661 gtatgatata gaaaataatg aagaagattt aaaggaagat aacaaagtac atttaaatga
     9721 tttaagcaga gtaaaatatg attacagaga gttaaaagac atagaaaacc aaacagaaga
     9781 gatgtgtctg ctagcagtaa agcaggatgg tactataatt gagtttgtaa aggacaagac
     9841 ttataaagta tgtatggaag cagtaaaaca gacatataaa tctcttaagt atataacaaa
     9901 tcaaaatgag gatatttgta tagaagcagt aaaacagaac tacagagctt tgtactacat
     9961 aaataataag acagaaaatg tactaattga ggctataaaa aatgcatcaa cttatgatgt
    10021 catggaagta tttaaatttg tggaagagca aacagaagat gtatgtctag catttataga
    10081 aagggcatct aaaaatgatg ttgcagagat attaaaagga ataaaagagc aaactccagc
    10141 catatgttta gaagctgtaa aaaaagatgg aaaatcttta gcttatgtaa aggaacaaag
    10201 taatagtata tgtttagaag ctgtaaaaga aaactacagt gcactatctt gtgtaaaaga
    10261 gcaaactgaa gaaatatgta tagaagcagt aaaacagaat gactttgcat tgtattatgt
    10321 aaatgaacag acagaaaaaa tatgcatgga agcagtaaaa agaagttata tggctctaca
    10381 gtatgtaaat aagcaaacag aagagatttg tttggaagca gtaagaattg atggaagagc
    10441 attacagtac gtaaaagagc aaacagaaga aatttgcttg gagtcagtta gacaaaatgg
    10501 aaaagtactg caatatgtaa aaaaacagac tgaaaatata tgtatagaag cagtaagagg
    10561 ttcatttgaa gaacttgaaa taaaagaaat attaagttat gtaaagatac caacagaaag
    10621 aatatttgta gaagcagtaa agcaaaatgg aaaaattctt aaatatgtag aaaatcagac
    10681 agaattaata tgtttagagg cagtaagaga aaactataat gctttagctt atgtaaagga
    10741 acaaacagaa aagatttgtt tagaagcagt taatcaaagt tatgaagcgc ttaaatatgt
    10801 gaaagaacaa acagaagaag tttgtctaaa agcagtaaaa caagattata gaatgttgaa
    10861 atatgtaaat aatcaaacag aaaaaatatg tttagaagca gtaaagcaaa attatagagc
    10921 tttagaattt gttgataatc aaacagaaaa agtttgtctt gaagcagtaa aacagaatag
    10981 aaaagcacta cagtatgtaa aacaaaaaca aagttaaaag gctaaaatat atggggaata
    11041 tatgcaaata aataaaattt gatattttgt aataaataaa atgggtatct caaaaggaac
    11101 tttttagttt gtgaggtact ctttttatat gaaatgaaat atatttttag ccttttaaca
    11161 aaggggaaaa ggtttgtttc tattttgaca taggcttttt atgcatataa agcttgataa
    11221 aattgtttta ttataattgt tttgttatat aaataataag aagaaaatta tataggtctt
    11281 tttttgacca gttttacatt tttaacaact agttttgtaa agtatattga attttctcaa
    11341 aaaagaatgt actctatata tgtaaaattt ttttggaggt aaaaatgttt aaaagatatg
    11401 ctgaaaaaat gacatctgtt ttaatttgta ataatatgat agataataat gaatctaaag
    11461 tctattctta tggatttgaa atattaatag ctttcatagt taatataacc accatgctgt
    11521 ttataggttt tttatttggt aaatttacat atgtattatt ttttttaatg tgttattgtc
    11581 caataagaca attttcagga gggtatcatg cagacaatta ttttagatgc ttattaactt
    11641 ttatttttat aattttatcc acaatcttaa ttatagaaaa tataaatata gacttattta
    11701 aaaatattat tatgattatt gcaagtgtta gttgggttgg aatttgtgtt ctgtgcccaa
    11761 tagaacatag gagtaaccct ataagtgata gggaaaagtt ggtttataag aaaactgcta
    11821 tatttatatc tacagtagtg ttattgataa caatttcaag tctttctttg agtatatttg
    11881 tagattattt cacatatagc gcatttgcca tgttttggat ttttgtgatg ttagtattag
    11941 gtaagttaaa agctaaagta taagtaaaat atgtatgagg gagagttaag ttatgaaaaa
    12001 gtttattgta agatttatga agtttgctag ctcattggca cttagcactg ctattttgtc
    12061 agctaatagt acctgtcctt ggattattca tcagcctaaa gtacccaaag aaatcagcaa
    12121 tctaaagaag acaaattaaa tacaagcact tatttataca aaattatcag gaaaaaagaa
    12181 attattatat tttaataaga ttaaaacaat ataaaatcag atacaaaatt tattttatag
    12241 gagcctaata cctatttggt aatatattag tgcgccaatt tagggggata tagtgtattt
    12301 aatgaaatga cttacggaga gtaagtcatt tttgtattgt atgtatttta tgtaaactta
    12361 tgatataatg aagggtaaag tttaattaaa ataataaaaa actttagtat agatagtaat
    12421 ttaggaggta gggtatgagt atgatacata aattttctat gaatggatat aatatcgttt
    12481 tagatgtgaa tggtggagct gttcatgtgt tagatgatgt tgcatacgat ttgttagatt
    12541 tttataaaga aaaaagtaaa gaagaaatat tagagatatt aaaatcaaaa tatcaagaag
    12601 aaaaaataaa tgaagcatat gaagaaatat taaatttgga aaaagagggt cttttatata
    12661 cagaagatac atatcaatat catccaagct ttgtacatag agagcctgta gtaaaagctt
    12721 tatgtttaaa tgtagctcat gattgtaact taaaatgtaa atactgtttt gcagctcaag
    12781 gtgattttgg aggagaaaaa gaacttatga gttttgaagt tggtaaggca gcaattgact
    12841 atttgattgc taattcagga agtagaaaga acctagagat agatttcttt ggtggagagc
    12901 ctttgatgaa ctttgaagtt gtaaaacaac tggttgacta tggaagaagt gtagaaaaag
    12961 attataataa aaatataaga tttacaataa ctacaaatgg tgtactgcta aatgatgaga
    13021 ttatagatta tataaatgaa aatatgcata atgtagtttt aagtttagat ggaagaaaag
    13081 aagttaatga taatatgaga ccaactttaa atgataaagg aagttatgat attacattac
    13141 cacgatttaa gaaacttgtg gaaaaaagag caaaagataa gtattattat ataagaggta
    13201 cttttacaag agataattta gatttttcaa aagatgttat gcattttgca gatttaggat
    13261 ttaaattaac ctctgtagag ccagtcgttg gagatgagag taatccatat gcacttagag
    13321 aagaagattt acctaagata tttgaagagt acgaaaaatt tgctgtagag tatgcagata
    13381 gacaattaca aggagatgga tttaaatttt tccattttat gatagaccta aatcaaggtc
    13441 catgtgtaat aaagagaata actggatgtg gagctggaaa tgagtatctt tctgtaactc
    13501 caaatggaga tatctatcca tgtcatcaat ttgttggaaa tgaagaattt aaaatggcaa
    13561 atatatttga tgaagaaata gtacttccag aaaatttaaa aaatatgttt agagaagctc
    13621 atgtatatac taaagaagag tgtaaacaat gttggaataa gttttactgt tcaggtggat
    13681 gtcatgcaaa tgcgataaac tttaataatg acatatcaaa accatatgaa ttaggatgtg
    13741 aaatgcaaag aaaacgtaca gagtgctcta taatgataca agctaagtta atgttagaag
    13801 gagcaaccaa ctaaatttat attttaaagg agttgtttat atatgattag agattattta
    13861 gaagataaac ctttaattga cgaaagtgta tttgtggcaa aatcagcaga tgtaattgga
    13921 aatgttaaaa taggcaaaga ttcaagtata tggtacaatg cagtagttag gggtgatgaa
    13981 ggaccaataa ctattggaga aaacactaat atacaagatt gttctatagt gcatggagat
    14041 acagaaacta taattggtaa caatgtaaca gttgggcata ggtctatagt tcatggttgt
    14101 aaaattagtg ataatgtgtt aattggtatg ggttcaataa tattggataa tgctgaaata
    14161 ggagaatata ctctgatagg agcaggtaca ctaattacat ctaataagaa gtttccgcca
    14221 ggtgttttga taatgggttc tcctggtaag gttgttagag agcttacaga agaagataaa
    14281 aaatatattg atgaatctta tgaatggtat ttagaggcag cacaaaatca aaaatattag
    14341 tttaaagagt ggttgtttca aaacatattt aaatctgttt tgaggcaacc actaatttat
    14401 tttattagat tttgttgata ttttagtttt caaaaagtat ttgattaaac cagatataaa
    14461 atgtcaaaaa taaaaattaa ctgagttaaa taattttaat aaaattggta gtatatgtga
    14521 taaaattaaa tattaaagag gatataggat attagaatat caattggtga aataatgtca
    14581 tatacgataa attaaggtgt aagtttttgg gtatacctat cttaggagtg aaaagatttg
    14641 agaataagtt atagaaattt aaagcgatat agagaaattg gatatgtttt aataaagtat
    14701 ggttttagtt ttattgtaga aagactaaat atagaaggaa ttgcctataa aataccacta
    14761 tttgacccac cagaagaaat aaaaaatatg acaactggtg agagaatgaa aagagtttta
    14821 gaagaactag gacctacata tataaaaata ggacagatat tgagtacaag aaaagacttg
    14881 ttagaccagg atataattga tgaaatatca aaactcagag atgatgtaga gaaatttgat
    14941 tcaaatatag ctattgatat atttaaagaa gaagtgggtt taagtataga agaaatattc
    15001 ttagaattta aagaagaagc aatagcagca gcttcaattg gtcaagttta tgaaggtgta
    15061 cttaaaacgg gagaagaagt aatcgtaaaa attcaaagac caaatataga gaaaataata
    15121 aaatctgatt tagagattct tagaactatt gcaaatactt taaaagattt aaaaaaggat
    15181 tttaatttag atttagtaca gatgattgaa gaatttcaaa ctcaattaat gagggaactt
    15241 gattatacat ttgaagctat aaatgcaact aaattctcta ggatatttaa aaatagtgat
    15301 gaagtatata tacctaaagt atattcagaa tataatacta agaaaatttt agtaatggag
    15361 aaagttaatg gtacaaagtt aagtgatgta gaaaaaataa ggagactagg ttataacaca
    15421 aaaactattg ttgaaatagg cgttaggtca ttttttacac aggtattatc tcatggattt
    15481 tttcatgcag acccacatcc aggtaatata ttcgtagttg ctaaaaataa gatagcatat
    15541 atagattttg gaatgattgg aattattgat aataaaactt taaaccaatt aaatgaaatt
    15601 gcattagcag gtgtagaaaa gaatgttgac aagataatat atctattgat agaaatggat
    15661 gcattaaatg gagaggctga tattaaggga cttagacagg atttacttta tctaatacat
    15721 tattactatg atatttctat agaaaaaata aatgtaacag atatattaaa tgaactattt
    15781 aggtttttta gacagtataa aatagtcatg cctgcacaat ttgtaactct tgcaaagaca
    15841 gttataacat tagaaggaac aagtagaact ttaaacacag atttttcttt tggttctatg
    15901 ggaaaagagt ttatgaaaca tcattataag agtaaattta accctaaaaa tgttgtttta
    15961 agttcaagac aaaatgtaga agaaatatta ctggacataa aaactattcc aaagcaatta
    16021 aaagcaattt taaaaaatat agaaagaaat aatataaaaa tgcaaattga agatgttaaa
    16081 atgacgaggt tagaaaattg tataatagag ctaacatctc aaatatcttt gagtttagtg
    16141 ttggcatcta ttatagtagg ttcttctctt ataattgcat ctccaaatat agaaaataat
    16201 atatggataa aatttactgc aatagcgggt ttctttatat cctttataat aggcctttgc
    16261 ttagttataa gaagtattag gtcaaaatac aaaaaagatt agggggaatt aaatttgaaa
    16321 aaatggatat tgaagtataa aggtaagata gaggaaagtg aatttagcaa aaaacttaat
    16381 atttccccgg aaatctgcca aattttaaaa aatagaggga tatgtactga aaaagattca
    16441 gagatattta tgaatccatc tttagattat ttaagagacc cttttttaat gaaagatatg
    16501 caaaaagctg ttgatagaat aaagttagca gttgagaaga acgaaaaaat ttggatttac
    16561 ggagattatg atgttgatgg tgtatcatct acatctatct tgtgcttata ttttaagagt
    16621 ataggttaca atgttgatta ctatattcct aatagattag aagaagggta tggtataaat
    16681 gaagatgcta ttaagcttat aagctctagg ggatgtgact tgattataag tgttgactgt
    16741 ggaattacat cagtgtctga ggtcaataca gcgaatgatt tgggcataga tgttataatt
    16801 acagaccatc atgaatgtca aagtgaaatt ccatcagcct ttgcagtaat aaatccaaaa
    16861 caagaagatt gtaattatcc atttgattca ctatgtggat gtggtgtagc atttaaaatg
    16921 attcaagcct tgactccaaa agaagaattt aaaactagta tgtataatta tttggaaata
    16981 gtaacactag caactatttg tgatattgtt ccacttatag atgaaaatag aataattgta
    17041 aaaaatggtt taaaatcaat gaaagaaggc aaaaatattg gattaaggga gcttataaaa
    17101 gtttgtggtg tagaaagtga taagataggt tcttctcata taggttttgc aataggtcct
    17161 aggataaatg cttcaggtcg attgggatat tcttacttag gagtagagct atttactact
    17221 caatcacaag aagaggcagt agaaatagct agtatattgg aagaaaaaaa taatgaaaga
    17281 cagatgatag aagctaagat gtatcatgaa gctgaggaaa tgctaaagtc taatagcaga
    17341 tacaatgatg acaaggtgtt ggttttagct aaagagggtt ggcaacatgg aataatagga
    17401 attgtagcat caaagttaac tgaaaaatac tacaaaccaa caattcttct aggtatagaa
    17461 aatggggagg ctactggttc agcgaggtca ataaaaggat ttaatatatt tgaagctctc
    17521 ataaagtgca aagacttgat gactaagttt ggtggtcatg aacaagcagc agggttatcc
    17581 ttggattcag acaatgtaga aattttagca aatgaaataa ataaatttgc agattataat
    17641 ttaactgaag atgatatgat agagaatgta aatgttgagt ttgaattaca agaaaatgta
    17701 ataaatttaa atcttgtaga agaattacat aagttagaac cctttggatt aaataatcct
    17761 aatcctagat ttatagttag aaattatatt ttaaaagatt tgaaagttat aggtaagaat
    17821 caacaacatc tgaaattaag catagaaaaa gagaaaagct atgaatgtat aggatttaat
    17881 atgagtcatt taaaatcaat gtataaagta ggagacaagg tagatgtact attccaatta
    17941 gatgaaaata actatatggg aaataggaaa gtacaatttt tacttaaaga tatacgattg
    18001 gctcgaccta aaagtgcaag taatgacaaa ctttcattga aattaatgtc aaaaataata
    18061 ccaaaagata ctcaaagcct gtataatata tctgtgtctg attttgaatt atttgatgga
    18121 aatacagata taaatatatt tgattatttt gaaaaagata cactaataat atctaattct
    18181 ataaatggat tttatagagc aatgtcagat atatcactta tagatttgga ttttaacata
    18241 aattacaata ttattgaaga tgttagcaaa aatactgata aattagagct tatattttct
    18301 cctaatattg ataaaataga cttaaaaaga tataataata ttattcttta tgattattta
    18361 tacaataaag gcgaatattc gtatatatat gagaataaaa gagaagaaag tgaaattata
    18421 aaatattata ataaaacaga cctattatat ttaaagaatg tagttagtaa tatagttcca
    18481 tcaagagatg aatttataac aatttataaa caagcactca taaaaaaaga aattgatttg
    18541 gatatggtaa atataaggga aacttttaat gtaattccat taaagttttt tacgatatta
    18601 aatgtattta gagaattaaa tttacttgac tttaatttaa attatgaaaa aaattctgta
    18661 ctcataagaa tattgccaaa accacagaaa aaattagact taaatgagag tttaatactc
    18721 aataatctaa aaaatttaga aaaacaatac aatagcagtt attaggaggc gtttcccatg
    18781 gatttaaaaa actttataag gaatattgat gatttcccaa agccaggcat agattttaaa
    18841 gatgtgacaa ctctatttaa agatggagat gcatttaaat atgcagtgga ttctatagtt
    18901 gaagaattga aagataaaga tgtagattta gtaataggac cagaagcaag aggatttttg
    18961 atgggtacac ctgtagcata tgctttagga gtaggatttg ttccaattag aaaacctgga
    19021 aagttaccag gtgaagttga gagttatgag tatggattag agtatggtac agatacttta
    19081 gagatacata aggatgcaat taaaaagggt caaaaagtag caatagtaga tgatttattg
    19141 gctactggtg gaactatgga agccgctgcc aagcttgtag aaaaattagg tggagaagta
    19201 gtttctatgc aatttttgat tgagctaaaa ttcttaaatg ggagagaaaa gttgtctaac
    19261 tatgatgtta attctcttat aaaatactag tacttttata atagttggta taacaccaac
    19321 tattttcata tatattaaaa ttttatacaa aataggtggt tatatgcatg ataaagaatt
    19381 acaagaaata attgaaaaaa taaaagttta tgcacctaat gcaaattttg aaatggttga
    19441 aaaagcctat aatcttgcaa aaaaagcgca tgaaggacaa tatagaaaat ctggggaacc
    19501 atactttatt catccattag cagttgcaaa tattttaata gagatggaat tagatattga
    19561 aactataaca gcaggtttac ttcatgatgt tgtagaagat acagaatata cctatgaaga
    19621 cataaaaaaa gaatttggca aagaagtagc ggatttggtt gatggagtaa ctaaacttgg
    19681 tcaaataaaa tatcgttcga aagaagaaac tcaatctgaa aatttaagaa aaatgttttt
    19741 ggcaatggcg aaggatataa gggttatact tataaaactt gctgatagat tgcacaacat
    19801 gagaacatta aaatacatgc ctccagaaaa agcaaagtat aaagcaaaag agacacttga
    19861 aatttatggt ggtatagctc atagattagg aatttccaag ataaagtggg aactagaaga
    19921 cagagcatta agatttatgg acccagaagg gtattatgat ttagttagta gagtatcaat
    19981 gaagagaagt caaagagagg attatattca aggaattgtt gaacttctta aagataagtt
    20041 taaagattta agtataccat gtgatgtata tggtagacca aagcattttt acagtatata
    20101 tagaaagatg caaaaaaaac ataagacttt tgaagagatt tttgatttaa cagcagtaag
    20161 gatattggtt gataatgtaa aggattgcta tgcagtactt ggtatagttc atacattatg
    20221 gagacctatt ccaggaagat ttaaagatta tatagcaatg ccaaaaccca atatgtatca
    20281 atcattacac accactgttg taggtcctga tggagaacct ttagagatac aaataagaac
    20341 tcatgagatg cataatatag ctgaaaatgg tatagcagcc cattggaaat ataaagaagg
    20401 tatctctagc aaagaatcaa aggttgaaga aaaacttcaa tggttgagac agatgatgga
    20461 gtgggaaaaa gatttaaaag acccacaaga gtttatggat gcacttaaag aagatgtatt
    20521 taatagtcaa gtttatgtat ttacgccaaa aggagacgta attgaattac cagcagaatc
    20581 aacacctata gattttgcct atagggttca tacaaatgta ggcaataaat gtgtaggagc
    20641 taaaattgat ggaagaatag ttcctattga ttataagctt caaaatggta atattgtcga
    20701 ggtacttaca tcatctaatt ctaatggacc aagtagggat tggcttaata tagtgaagac
    20761 cccaaatgca aaaagtagaa ttagacaatt tttcaagaag gaaagaagag aagaaaatgt
    20821 tgaccgtgga aatacaatat tagaaagaga atttaagaag catgatatac cacaaaaaga
    20881 ccctcttgtt gaaaaatata tggcattagt tgctaagaaa tttaatcagc caagtgttga
    20941 ggacttaatt gccactgtag gttatggtgg tataatgtca tctcaagtgg tatctaaggt
    21001 aaaagaccta tatttaaaag aggttaaaaa agctaaaaag gaacaacaga tacaagaaga
    21061 agatttaaat aaacacaata taagtgaaac agaatacagt aaaaagagaa agaaaaacgc
    21121 atcacaagga attatagtaa aagggcttga caatatctta gtaagatttg ctaagtgttg
    21181 taatcctctt ccaggagatg aaattgtagg atatattact aagggtaggg gagtagccat
    21241 acacagagct gattgcccaa atgcaaacaa taatggtgat ttcttccaaa atagattggt
    21301 ggaggtttca tggagttcag caggaaaagg taagtttgaa gcggaaattc aaataaaagc
    21361 tattgataga agaggaattg ttaatgaaat aactcatatt gttgcaatgg ataaacttag
    21421 tttaaacgga ataaatgcaa gaaaaggtaa ggatagtatt gtaagtataa acttacttgt
    21481 agaggttaat gacacagagg aattaaaaat tctaatgaaa aaattaaaag caattcctgg
    21541 tgtagaaagt atttatagaa tgacaaatta gagttaaaat aatagggagg aaactatgag
    21601 agcagtagtg caaagagtat cttcatctaa agttacagta gatgaaaaca ctataggtca
    21661 aataaataag gggttactag tattgttagg agtgactcat gatgataaat caagtgatgt
    21721 agattatatg attgataaaa tattgaatct gagaattttt gaagatgaaa atgacaagat
    21781 gaatttatct ctaatggata taggtggaga gcttttagta gtatctcaat ttactttata
    21841 tggagattgc agaaaaggaa gaagacctgg atttagtaat gcagctaggc cagaacttgc
    21901 aaataacctt tatgaagaat ttgtaaaaaa agcaaaagat aaaggtgtga cagttggtac
    21961 aggtcaattt gcagctcata tgatggtaga gttaactaat gatggtccag taacaatact
    22021 attagactca agtaaatctt tttaaggggg aaaactttaa tgattataaa aaaatttgta
    22081 gatagttatt ttggagtaaa tacttatgta ttaggtgatg aaaagactaa aaaatgtgca
    22141 gttatagacc caggtggaag tttagtagag acattaagtt ttgtaaaaga aaatgaatta
    22201 aatattgaat atattatcct tactcatggg catggagacc atataggata tgttaaggat
    22261 attaaagaaa aaactggtgc taaagtagtt gcacatgttg atgaaaaaga attattaaat
    22321 gacaaaaata aaaatctaag ttatacaatg agatgtggag cgcaagagtt tgatgcagat
    22381 atctatgtaa atgataaaga caagcttgaa cttggtgaat taaaaatgac tttcttgcat
    22441 actccaggac atactcaagg atgtatgtgt ataagagttg aagatgaaat gttcacagga
    22501 gatactttgt ttgctggaag tattggtaga actgatttat atagtggaga ctttaaccaa
    22561 atggaaaaat cacttaagaa attatgtaaa tatgaagatg ctgttagagt atatcctgga
    22621 catggaccaa ccagtactct aggggttgaa aagaagacaa atccttacat gtaattttat
    22681 attagtattt aaaaaagtat aaaatattta ataactatat tagtgagggg gttcttctac
    22741 tgttaaatag aaggagttaa gttgatgttg tatgtttttt taaaaggaca tgattataag
    22801 tatgaagtag cagaacttat aaaattgttt actagtgaat ttaagtttaa agactctaat
    22861 gctaacaata atatagagtc aaagcattta ataaatagat tgatttatga gaatggtgta
    22921 ttattctcaa gtactgaata ctatgaaaat ggaaacttaa agtacgagtc aatacagaat
    22981 atagaaaata tgaatcttga atttttagat ggatttgata gcagcaaaac gattgaagaa
    23041 tttcttaata gcctagatag tgaaagtaag aggaacttta aaaagctgtg taaagaaaat
    23101 atcaaaaaaa gcatgttttt agtgcttaaa caagtattta attcttatgt accatgggga
    23161 attttgactg gtattagacc agtcaaaatt gtccacaatt taatggataa aaagctagat
    23221 gataatagca taagaaccat tctaaaagat aattatttta taatagatga gaaaatagat
    23281 ttagctcttg aaatagctaa aagagagcga ttatttatgt atcctataga taaaaataaa
    23341 atttctcttt atgtaagtat tccattttgt ccaactagat gtgtatattg ttcatttcct
    23401 tcaaattcat taaaacaatt tggacattta aaaagagaat atgtttataa attgattgaa
    23461 gaaattaagg ggtttgccaa agttattaag gatactaaga aagaagttga aactctatat
    23521 attggtggtg gaactccaac tacacttgat gaggaagaat tggatttgct tataaattca
    23581 ttgttcaatg aattagattt aagtaaaatt aaagaattta ctgtagaagc aggtagacct
    23641 gacactatta cagaacaaaa actaagagtg ttaaaaaaac acaatgttag tagaattagt
    23701 ataaatcctc aaactatgaa tgatgatacc ttggtaaaga ttggaagaga gcatagagta
    23761 tctgatttag tagattgctt taatatggct cgtagaatgg gatttgataa tataaatatg
    23821 gatataattt taggtcttat agatgaagac ttaaatatgg taaagaacac attggaggag
    23881 ataaaaaaac tttctcctga aagccttaca gttcatacat tagccataaa gagagcatct
    23941 aacttaaata taaatatgga taggtataaa gaacatctaa cacaatatga agaaatggta
    24001 aaaatgatag atttatcaat gaaatatgca aaagatatgg gattaaatcc atattatatg
    24061 tacagacaaa aacatatgtt gggaaattta gaaaatatag gttatgctaa agaaggatat
    24121 gagtgcattt ataatataca gattatggaa gaaaaacaaa gtaactatgc attaggtgca
    24181 ggtgctatat cgaaatttgt ctatgtagat gaagatagaa ttgaaagagt agaaaatgta
    24241 aaaaatgttg aacaatacat tgaaagagta gatgaaatga taaaaaggaa gaaagaagag
    24301 gtatataaaa atgttgacta aagctcctag aggaactaaa gacataactc caaaagaggc
    24361 ttacaaatgg cgttatgtag aaaataaatt tagagaaata tgtgctttat atggatatga
    24421 agaaatggta acacctatat ttgaacatac agaacttttc aagagaagtg ttggagatac
    24481 tacagatata gttcaaaaag aaatgtattc ttttaaagat aaaggtgata gagaaattac
    24541 cctaaagcca gaaggtacag caggtgtagt aagagcattt atagaaaata aattatatgc
    24601 agatactcaa ccaacaaaat tattttatgt gacaccttgt tttagatatg aaagaccaca
    24661 agctggtaga caaagacaat ttcaccaatt tggaatagaa gcacttggaa gtgatactcc
    24721 ttcaatggat gctgaaataa tcgcactagc tgttcaattt tttaatgaag ttggattaaa
    24781 tgatttggta gtgagtataa attcagttgg ctgtccagtt tgtagaaaag agtacaatgc
    24841 tttattaaaa gagtatcttg actcaaaagc tgatatattg tgtgatactt gcaatgagag
    24901 aagagaaaaa aatccaatga gggttataga ctgtaaaaat cctacatgta aagaaaatat
    24961 caaagatata ccatttattg cagaccattt gtgtgatgat tgtaaatctc actttgataa
    25021 acttcaagaa tacttaaagg aaatgaatat aaactttgta attgataaaa caatagttag
    25081 aggcttagat tactatagaa aaactgcttt tgaaattata tctaatgata taggagctca
    25141 aagtactgta tgtggtggag gaagatatga tggtcttgtt gaacaattag gaggaccaaa
    25201 aggtataagt ggaattgggt ttggactagg aattgaaaga cttttactta cacttgaagg
    25261 taatggaatt gaaatagaaa accctcaatc tacggatata tttatagtaa ctataggtga
    25321 agaagcaaat acaagaagct ttaaattgtt aaaagacttg agacaaaatc atataagtgc
    25381 agataaagac catatagaga gaagtgttaa agcacaattt aaatattcag ataaaataaa
    25441 ttcaaaattt acaatagtaa ttggagatga tgaacttaaa aatgacactg cgacattaaa
    25501 aaatatgaaa acatcagagc aaactactgt aaaattaagt acattagttg aagaattaaa
    25561 gcaaaagctg taaaataaga aagtatgatg ttttgtgtat tagataaata tatagcatta
    25621 aaattaaata aaatggaggt agtatcttgg aaactttaaa gggtttaaag agaactcact
    25681 actgtggaga gttgagagaa aaaaacataa atgaagaagt cgtacttatg ggatgggtac
    25741 agaaaaaaag aaatttaggt ggtcttgtat ttgttgattt aagagataca agtggattat
    25801 gtcaaatagt ttttgataca gatgtagata aagaagcttt tgaaaaagct gaaaaattag
    25861 gggctgaatt tgtaatagct gtaaaaggaa aagtatgcga aagacaatct aaaaacccta
    25921 atatgccaac tggtgatata gaaatatttg caactgaact tagactttta aacaagtcag
    25981 aaactccgcc tatttatata aaagatgatg atgatgtttc tgaagcacta agattaaaat
    26041 atagatactt agatttaaga aaaccatcta tgcaaagaaa tttaaaatta agacataaag
    26101 taatgaatat tacaagaaat tacttatcaa acaatagatt ttgcgaaata gaaacaccat
    26161 ttttaatagc tccaacacca gaaggagcaa gagattatct agtaccaagt agagttaatc
    26221 caggaaaatt ttacgcatta ccacaatcac cacaattata taaacaatta ttgatggtaa
    26281 gtggtatgga tagatatttc caaatagtta aatgctttag agatgaagac ttaagagcag
    26341 atagacaacc agagtttaca caaatagact gcgaaatgtc ttttgttgag caagaagatg
    26401 ttatgagtat gattgaaggt ttgcttgaag cgatatttaa agaagtttta gatgtagaat
    26461 tggcacttcc acttcctaag atgacttatg cagaagctat gtctaaatat ggttcagata
    26521 aaccagatac tagatttggg tatgagttaa cagatatatc tgatgttgtt tgtaactgtg
    26581 gatttaaagt gtttgctgat gctacacaac caggaaaaag tgttagaggt ataaacgtaa
    26641 aaggcaaagc tgacgacttt actagaaaac aaatatcatc actagaagaa catgctaaga
    26701 catatagagc aaaaggtctt gcttggatga aagtaggaca agaaggagta acttctccaa
    26761 tagcaaaatt cttcaatgaa gaagaaatga acgctatact tacaagaatg aatgctgaag
    26821 taggagattt acttttattt gtagctgata aaaactcaat agtatttgat gctttaggac
    26881 aagttagact tgaagtagca aataggctta atctattgga taaaaatgta tataacttac
    26941 tatgggtaac tgagttccca gtttttgaag aagatgaaga aacaggcaca ttctcagcaa
    27001 tgcatcaccc atttacatca cctatggatg aagatttaga caagttagaa gaaggtgata
    27061 agtcatcttt aagagcgaaa gcttatgata ttgtattgaa tggttatgaa ataggtggag
    27121 gaagtgttag aatatcaaat tctgatgttc aatcaagaat gtttaaagca cttggtttta
    27181 cagaggaaag agctaatgaa aagtttggtt atttattaga agcatttaaa tatggtactc
    27241 ctcctcatgc tggtctagct tttggtcttg atagacttgt tatgttactt gcaggaactg
    27301 ataatataag agaagttatt gcattcccta aaaatcaaaa tgctgtttgc cctatgacaa
    27361 atgctcctac attggcggaa gatgagcaat tagaagaatt aagtataaaa gttgatataa
    27421 aagataatga ataatatgta gttgcaagaa actaaaatgt aaaaagtctg gatataaaat
    27481 atatttatat tcagactttt tttattagaa ttattttata ttttttctct atatattttc
    27541 ataaattata cttatattct taaccaatat aattaaaata ctatgtttaa aaattttaca
    27601 taaacttggt tgttttagaa actacttctg taacgagtac acgattttaa ttaaagaata
    27661 tataacaaat ttgaaataag tctaatatat gacttaatag attaa
//
LOCUS       DAYAOX010000028        27537 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.028, whole genome shotgun sequence.
ACCESSION   DAYAOX010000028 DAYAOX010000000
VERSION     DAYAOX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.028"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            120..560
                     /locus_tag="ACGI57_000979"
     CDS             120..560
                     /locus_tag="ACGI57_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HFG9716065.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
     gene            1205..2512
                     /locus_tag="ACGI57_000980"
     CDS             1205..2512
                     /locus_tag="ACGI57_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716066.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    2598..2860
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2986..3789
                     /locus_tag="ACGI57_000981"
     CDS             2986..3789
                     /locus_tag="ACGI57_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9716067.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3827..4438
                     /locus_tag="ACGI57_000982"
     CDS             3827..4438
                     /locus_tag="ACGI57_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFG9716068.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            4470..5141
                     /locus_tag="ACGI57_000983"
     CDS             4470..5141
                     /locus_tag="ACGI57_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFG9716069.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            5440..5802
                     /locus_tag="ACGI57_000984"
     CDS             5440..5802
                     /locus_tag="ACGI57_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFG9716070.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            6238..6939
                     /locus_tag="ACGI57_000985"
     CDS             6238..6939
                     /locus_tag="ACGI57_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HFG9716071.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDELCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            7210..8028
                     /gene="larE"
                     /locus_tag="ACGI57_000986"
     CDS             7210..8028
                     /gene="larE"
                     /locus_tag="ACGI57_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HFG9716072.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            8083..9231
                     /locus_tag="ACGI57_000987"
     CDS             8083..9231
                     /locus_tag="ACGI57_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFG9716073.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            9367..10755
                     /locus_tag="ACGI57_000988"
     CDS             9367..10755
                     /locus_tag="ACGI57_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9716074.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            10784..11482
                     /gene="walR"
                     /locus_tag="ACGI57_000989"
     CDS             10784..11482
                     /gene="walR"
                     /locus_tag="ACGI57_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism DNA-binding response
                     regulator WalR"
                     /protein_id="HFG9716075.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            11485..12918
                     /gene="walK"
                     /locus_tag="ACGI57_000990"
     CDS             11485..12918
                     /gene="walK"
                     /locus_tag="ACGI57_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFG9716076.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            12940..13677
                     /gene="truA"
                     /locus_tag="ACGI57_000991"
     CDS             12940..13677
                     /gene="truA"
                     /locus_tag="ACGI57_000991"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFG9716077.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHSPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    13788..13995
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14248..15447
                     /locus_tag="ACGI57_000992"
     CDS             14248..15447
                     /locus_tag="ACGI57_000992"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFG9716078.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            15694..16740
                     /locus_tag="ACGI57_000993"
     CDS             15694..16740
                     /locus_tag="ACGI57_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HFG9716079.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTGQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            16848..18011
                     /locus_tag="ACGI57_000994"
     CDS             16848..18011
                     /locus_tag="ACGI57_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9716080.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     NRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(18100..18480)
                     /locus_tag="ACGI57_000995"
     CDS             complement(18100..18480)
                     /locus_tag="ACGI57_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HFG9716081.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(18483..19142)
                     /locus_tag="ACGI57_000996"
     CDS             complement(18483..19142)
                     /locus_tag="ACGI57_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFG9716082.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(19248..20222)
                     /locus_tag="ACGI57_000997"
     CDS             complement(19248..20222)
                     /locus_tag="ACGI57_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFG9716083.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(20262..20609)
                     /locus_tag="ACGI57_000998"
     CDS             complement(20262..20609)
                     /locus_tag="ACGI57_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716084.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(20827..21783)
                     /locus_tag="ACGI57_000999"
     CDS             complement(20827..21783)
                     /locus_tag="ACGI57_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFG9716085.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            22228..22596
                     /locus_tag="ACGI57_001000"
     CDS             22228..22596
                     /locus_tag="ACGI57_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HFG9716086.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            23021..23266
                     /locus_tag="ACGI57_001001"
     CDS             23021..23266
                     /locus_tag="ACGI57_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716087.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
     gene            23365..23628
                     /locus_tag="ACGI57_001002"
     CDS             23365..23628
                     /locus_tag="ACGI57_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HFG9716088.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(23913..24860)
                     /locus_tag="ACGI57_001003"
     CDS             complement(23913..24860)
                     /locus_tag="ACGI57_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9716089.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLANTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(24863..26494)
                     /locus_tag="ACGI57_001004"
     CDS             complement(24863..26494)
                     /locus_tag="ACGI57_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9716090.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            26624..>27537
                     /locus_tag="ACGI57_001005"
     CDS             26624..>27537
                     /locus_tag="ACGI57_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9716091.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNS"
BASE COUNT        10739 a         3024 c         4421 g         9353 t
ORIGIN      
        1 ttgtaaataa acagcaaata attttaggct ttttatttaa tatagttttt atttatattt
       61 atttttgaat atgttatact ttgtttaaat aaatatttgt aatgagggag atgagaagat
      121 tgaagaatct caatttactt attaataaat tatattcaaa aaatcataat gaagcatata
      181 aaactttttt attcttagaa aatgaaagtt taaaatcaaa tattacatat tgtttttttg
      241 actcattcct agaaatgatt gataacgaaa actcatatat tcgaacaagg ggattgttat
      301 taatctctgc aaatgctcag tgggatattg ataataaaat tgaaataaat atagatagta
      361 tactaagtca tatcgtagat aaaaaacctt ctgtatcaag aatgtttatt aaatccatac
      421 ctaatattac aaagtataaa gaaaatctta ttaatcgcat aaaaatggaa ttaagtaatg
      481 cagatataag tatttataat aataatatga aaccattagt agaaaaggat ataaacgata
      541 cattatctaa tattagttaa atattaatat agaaattgaa tattatctag ttacaattat
      601 ttataactaa gataataaaa caacaatggt gctaatttat atgtataaat taattatgaa
      661 aaatttatat aagtaaaata tattaacata aaggtaaaat tttgtatact aaaagggaat
      721 atttacagtt taatggcagt tttaaaataa taaattaaag ctgtcatttt tatttaaaaa
      781 tttttagaaa aagtacaata gtgttaatgt gtgaattaga taaggtgtat ataatagttt
      841 agatgatatg atataaaata tgttgtaaat taaatgtttt ctttgacaat atatacacaa
      901 ggatgataat atctatatga ataaaagata gtgtgtaatt tgataatatg taattataac
      961 attatgatat aattacaata aagtatgtaa taatctgtag aaatttcaag ctagttatct
     1021 aagttttcgg ataactaaat ctaaacggaa tacataatgt attaaataaa tgatgatact
     1081 aaaatgatac tatttttata gaaatttatt gaggtatata attaatgaaa ataatatatt
     1141 aaattatata aattaaatta tataatatat tgagttttac ataaaagcaa aggtgggaat
     1201 aaaaatgaaa gaaattgctg atgctataac atcagagtat ttaagctatg gtatcaatga
     1261 agatattagt atctatgaag gtagattttg catatattta gataaaaaat atagatgtaa
     1321 tgggaaaatt tattataaaa tgacacctcc aatatctata agttttaaag cagacatagg
     1381 ttgtgttgaa gagatagaca atgaagatga caatttagct ttagattatg ataatgctat
     1441 tcttgaagtt catggatata aaattatttc tataacaata aatactttaa gtgaatttag
     1501 tgtagaaggt tatatcaatg atgactgtat aaaatctaag aactcctatg ttgaatatgt
     1561 agattttaat ataataaact tagataaaat acctggaaaa ctcattaaat acaatgataa
     1621 ggtatacgca ggtagaattg aatttgatat aaatgattat gttgtaacta tagacaagcg
     1681 atatgattat agaaaagaat taaaatcaga attaaagtct aaatctggag caataataac
     1741 tcatataggc aggattagaa gaaaagatgg tagaattttt agaacaaata atacaataaa
     1801 tcttttagat aggatatcaa cagctttgag ttttatgtgt ggaagatatg tagggttttg
     1861 tttggccaaa gggtatagaa gtggtaatga agtttataga atatggaatg aaaatcaaat
     1921 atctcctttt agatatgtac caacatggtc agatacactt tcaaattatc ataatatgga
     1981 aaaatatata agccttatgt gtaaaaagct agaagatttt tattatggtt ctgctataaa
     2041 gagtgttgtt gattggtaca tagaatctct tggaagtgct accatggaaa acaatataat
     2101 atctgtacaa attgcacttg aaactttgtc atatgtaatt ctagtggaac aaaataagat
     2161 acttacagat gaagcatttg actgtaattt ggcatctaaa aatataagac tcttacttga
     2221 tacatgtaag ataccatatg gtaaacatga attaaatata tttgataata taataaaaaa
     2281 taaatttgat gatggagtgg atttagttat ttatttaaga aatagcatag ttcatccaag
     2341 tagaaagact catagagctg tgttagaggt tgaagatatt tggaatataa tttcaatagg
     2401 aacgagatat attgaacttg ttttactctt tattttaggt tatagaggag aatattcaaa
     2461 cagattagta gaaagatgtt atggtgaagt ggaagttgta ccttggaact agatttttat
     2521 atttatttta aaatatatat tgacatatat tgccttatgc tataaaatca atatcataat
     2581 aataattcag aataacatta aatgctaaga aaagaaatag taaaagtagt taatcttaaa
     2641 cagagagttt ccgattggtg agaggagcaa tagattttac tttgaacaca tctttgagca
     2701 ggactctgaa agagtagaaa tactttatta ggagatttcc ggaatccttg cacccgttat
     2761 agtgctagag tataataatt ttattgtact taaagaggtt agtgccgtga ggcattaatg
     2821 aacagaggtg gaaccacgag agttaactcc cgtcctctag cagaagctag aggcgggagt
     2881 tttttaatat aattttttaa gcgcgcagag aattatattg caatagatac actttttaaa
     2941 aaataatcaa aatattttaa aaattaaaag acgagggaga atattatgaa aaatatatta
     3001 aaaaaagtag gaatatttac aattatgtta ggtttattag gtggagttgt aggttgttca
     3061 aaaccagata atgaaaaaga taaggatgct tcaaaggaat ctaaaaaaga agtagttgta
     3121 gggtttgata atacgtttgt accaatggga tttttagatg aaaagggaga tacagtaggc
     3181 tttgacgtag acttagctaa agaaacattt aaaagacttg gaatggaagt taagtttcag
     3241 cctatagatt ggtcaatgaa ggaaactgaa ttaaatgatt caaaaactgt agatgtatta
     3301 tggaatggat attctataac agacgaaaga aagaaaatag tatcatatac agaaccatat
     3361 ttacaaaata aacagataat agttactcta agtgactcaa aaataaattc aaaagctgat
     3421 ttaaaagata aagaagttgg aacacaacaa ggttcaacag cacttgatgc agtggaaaag
     3481 gataaagatt ttatgaatag cttaaaaggt ggagcaccag ttctttatga tacttatgat
     3541 aaagcactta gagatttaga gataggtaga actagtgcag ttgttggtga tgaagtttta
     3601 attagatact atatgggaca aaagggtgaa gacaaataca aggtattaaa agacgatttt
     3661 ggattagaag attatgttgt agcaacttct aaagaaaatc cagaactttg tgagaaaata
     3721 aatgaaacat taaaagaaat gaaaaaagat ggaacttttg acaagattta tgataagtgg
     3781 tttaaataga agataagtta gctgttaaga agggggcact aaaggcatgt tacaaggttt
     3841 agagatagtt atagcaatgt tttgtataac attgatagta tcaattccat tggggattgg
     3901 agttgcattt ttaagattat caaagaataa attagtaagt ggtattacac aatgttatat
     3961 attgataatg agaggtacac ctttactttt gcaaatgata gtcatatttt acggattacc
     4021 tcttttagga attgtatttg acagatttac agctggagta gtagctttct tcttaaatta
     4081 tgcagcatat tttgcagaaa tatttagagg tggaatacag tcaatcgata gaggacaata
     4141 tgaagcttca aaagtactag gatttgataa gtttactatg tataagagag taatctttcc
     4201 acaagttttt aaaagaatac tagcacctat ttcaaatgaa gtaatcacat tagtaaaaga
     4261 tacatcttta gtatatatat taggattaaa tgatatttta agaatatctc aaatagcaat
     4321 gaacagggaa gcaagtctgt taccattatt tgaggcagga gcgatatatc ttatatttgt
     4381 agctatactt actaagggat ttgaattact tgaaaaaaaa tattcttatt atagataaag
     4441 ttacaatatt ataggaggag gttttttata tgttgaaaat caaaaactta aataaatcat
     4501 ttaaaaaaaa tagagtactt aaagatattt cttttgagtt agaagaaggt cagattggag
     4561 ttttacttgg taagtctggt gcaggaaaga ccacaatatt aaggtgtata aatggtcttg
     4621 aagaatttga tagtggagag attataatag ataatgaggt aataaaaaat aagagagata
     4681 tggcaaaaat acgaggaaaa ataggtatgg tttttcaaaa tttcaactta tttcctcata
     4741 tgacagtcct tgaaaatata attgaatccc cagttaatgt ttttaaagtt cctagaaaag
     4801 aggctgaaga gagggctaga gaacttttaa gattagttga tttagaagat aaattaaatt
     4861 cttatccatt tgaattgtca ggaggacagc aacagagagt tgctatagct aggtcatgtg
     4921 cattgatgcc aaaagtcctt tgttttgatg aacctacttc tgcacttgat attgatacta
     4981 ttcaaagagt agtcaatata atgaacagac ttaaagacaa aggtatgaca atattaataa
     5041 ttacacatga tgttgtcttc tcaaataatg ttgcagataa aattataagt ataaaagatg
     5101 gtatagtaga aaatgttcaa ataaaagaaa aaattgttta gagttttata aatataaact
     5161 agggtagaat tttataatat aaataaaggg caagtaaaat caaatatact atgccctttt
     5221 gttatctcaa aatttaatct tatattttat gtttaagtta cattataatt tataataaaa
     5281 ataaaaggac tatttaaata tataatgtga ataaatgttt aataatttga aatatgataa
     5341 ttttttatgt tgaaagctta taatgattat gatataaatt ggttatagta tacaatttat
     5401 gggtataaca ataaaaaatt agaaaggaat ttttataaaa tgattaaatt atatggatat
     5461 acaaaatgct ctactgttaa aaaagctaaa aattggctta aggaaaataa tttagaattt
     5521 gaagatatag atatggttca aaaccctcct agtaaagagg agttaaaatc aatttataag
     5581 actagtggat atgatataaa gaaatttttt aatacaagtg gtatgaaata tagagaattg
     5641 ggtcttaaag atattgtaaa aactgaatct gatgataaac ttttagaaat attggtaagt
     5701 gatggtatgc ttattaaaag accattactt ttggatggta aaaatgtatt attgggcttt
     5761 aaagaagatg tatggaaaag tactttgcta aaagaagatt aggaagagat aggtgtgttg
     5821 attaaaatta ttaattaata cacctataaa tttatgtatt aaatctaatt tttataaata
     5881 ttaattagca aattcaaatc aaaaaaattc atatcaacaa acatattact tcttattaca
     5941 aattatatca tatgttttat attaaaatat tataatatta caacgaattg ttacaaaatt
     6001 acttattatt aaccatgaac atttgttttg tgctatcata tttatataag ggaattgtca
     6061 taaaaaatat cttgttcata cagttttata tagaaaaaag aaatgaatct tttctttata
     6121 taagtatatt ttgtaaattg cctctatatt tatagtttaa ataatagtgg tattaaaaat
     6181 ctgacaacaa aaataaaact ttcaaaaaat taaatctaaa aagaatagga gataaatatg
     6241 agtattgaag atttatatga ttttgaatgt gttcctttga acaatgagat tgatattgat
     6301 gttttatttg aaaagatgat agaagatgga aaagaaaaag aatatacacc aattatagtc
     6361 attgatgaaa aagtagggct attaagaaaa aatatagata tgattactaa agagtatggt
     6421 tctcttgaaa actatagaga gtattgttta acaaaatata atgaaattga tgtaaatcaa
     6481 ttttttgatt ttaggaagag tgagatttca gaattattaa aggagctaat tgatttagaa
     6541 gatgattttt atgattataa acattttaga ccaattatga atatagatat atttcatgaa
     6601 tataacaaag tttacatagc taaaattcca acaattaaac catatgaagt atttgcatac
     6661 atacctattg gcggatttaa tgattgtcct agagatgaag aacatatagc tattgcaaaa
     6721 tattggtatg aaaagcatgg aatctttcca atcgcaattg gatgtgatac agttcaatat
     6781 tctgtaaaaa atttaactaa agatggtaaa aaatttgatg aattatgtat tgaattagta
     6841 ttttattgtg aggatattat tatacaaggg tatgaaacct taaaggctct taaggatgta
     6901 ttaaaacgct ctacaatagt gcttttttgg tgggattgat attccatcat ttagatagta
     6961 ttaaattgtt agtcaccaag aatccacttg ctttagttat ggggagtgtg tcaaactagg
     7021 tttttataca cagtgtagca tagtaatatg tttaaattac aaaggttaaa aaaatacttt
     7081 aatattttta atattataat taatagattt aaaggtaatt ataatttata aaataaatac
     7141 tttatatgtt ataatcatta agaatgtaaa aaaataatat atagttttat agttcatatt
     7201 tttccagata tggaggtaaa ttttgtgaag gaaaaagaaa aattagataa attaaaaaaa
     7261 atgttacttg aattaggcag tgttgtagtt gcttattctg gtggagtaga tagtaatttt
     7321 ttgcttaagg tcgcaaaaga tacattaggt gaaaatgtta tagctgtgac tattcatgca
     7381 atgatgcatt caagtaggga aattgaagag gctaaacaat atactcaaaa ttttggtgtt
     7441 aaacatatca tattaaatat agaaaacttt gatttaaaag aatttaaaga aaatggtgtt
     7501 gatagatgtt atcattgtaa aaaatatata ttctctaaaa taaaagaggt agcaaaagaa
     7561 cataatataa agtacatagt agatggaaca aatatagatg atttaggaga ctatagacct
     7621 gggcttaagg cattgagtga attaggagta attagtccat taaaagatag tagtttaaaa
     7681 aaagaagaaa ttaggtcgct ttcaaaaaca ttaggattaa atacttttaa taaaccatca
     7741 tttgcatgtc ttgcgagtag gataccttat ggagttgaaa taacagatga aaatttaaga
     7801 atagtagaaa agagtgaaga atatctatcc aatttaggtt tttctcaatt tagagtgaga
     7861 atgcatggag atatagctag aatagaagtt ggacaggaag aactaggtaa attttttgaa
     7921 aataacaatt ttaataaagt tgatactaaa ttaaaaatat ttggctttaa atatgttaca
     7981 ctagatatgt caggatataa aatgggaagt atgaatttaa atgtgtagat tattttaaaa
     8041 ataaataagt tttatagtta atataaggaa acaggaggtt ttatgaaaaa ttatacgtta
     8101 atatcgccat gtttttttgg aatggaaaaa atgctggcta gagagattac taatttagga
     8161 tatgaaataa taaaaacaga agatggaaga ataacatata aaactgatga gtttggaata
     8221 gcaaagtcaa acatgtggtt gagatgtgct gaaagagtac atcttaaaat tgctgaattt
     8281 gaggcaaaaa gctttgatga attatttgaa aatacaaaaa gaataaactg gtctagatat
     8341 ataccttatg gagctcaatt tcctatatca aaagcttcct ctataaaatc aaaattatac
     8401 agtacaccag atgtacaagc catagtaaaa aaggctatag tagaaagctt aaagaaaagt
     8461 tatttggagg atggtttgct taaagaagat aaggagaaat accctatttt tgtatttata
     8521 cacaaagata aagtaaccat ttctatagat acaacaggag atgctttgca taagagagga
     8581 tatagagaaa aggcaaataa agctccaata agagagactt tagctgctgg gcttatttat
     8641 ttaactccct ggaaagcagg tagagtttta gtagacccta tgtgtggttc tggtactata
     8701 ttgattgaag ctgctatgat tggtataaat atggcacctg gcttaaatag agagtttata
     8761 tcagaaaagt ggagaactct tgataagaaa atatggtggg atgtaagaaa agatgctttt
     8821 aataagatag ataatgaatc taaatttaag atttatggat atgatataga tgaagaatct
     8881 atagatatag ctagagaaaa tgctgaaata gcaggtgtag atgaatatat agagtttaat
     8941 gtaggagatg caactcaatt taaaagtgaa gatgagtttg gatttataat cactaatcct
     9001 ccttatggag aaagattaga agataaagac agtgttaaac agttgtataa ggagcttggt
     9061 tatgcattta gaaaattaaa aaattggtcg tattatttga taacttctta tgaagacttt
     9121 gaatatgagt ttgggcaaaa agcagataaa aaaagaaaat tgtataatgg tatgttaaaa
     9181 actaactttt tccaatatcc gggtcctaag cctcctagaa ataataaata agttttacat
     9241 gatttatttg taaaatataa aattttatta aaataagtaa aacattatga tgccttgaaa
     9301 cgtctaatta ttgtgaggag atttttgtga acaaatttaa agatttatag gattaggagg
     9361 agttaaatga aagtgttaaa gcttattact gtagtagccc tgatgatgtt atttgttact
     9421 ggatgtaata taaaaactga atctccagaa caattagcga agatacctga ctatgatgat
     9481 acaaaaaaag tattgtatga tggtattgac cagttactaa aaccagattc atctatgata
     9541 ttgccttcta atacaaaaga agtaggaaaa ataaataagg tagatttaaa cagcgatgga
     9601 attgatgaat tagtagtatt tgaacaaaaa gaagatttaa gtaataatgt gagtcaggta
     9661 gggtttgtaa ccttgagtta caatggagaa aaatacgtac ttggagacca ttttttagaa
     9721 aatggcgaat caatagaata tgctaatttt tatgatttgg actcagatgg atataaggaa
     9781 gttatcttat tggttaaatc caaagacaaa acgaatatgc atatatataa agtagtagat
     9841 aatgaaataa caaaaatata tgacttggat gcatcatggc ttcaaaataa agaagatttt
     9901 aatgatatga aagtaaaaat tggatatata gatggagatg acaagttaga catattgatg
     9961 cttcatttga ataataagac taatgaaatg tttgcaagtg ttgcaaattt tgatgggaaa
    10021 atgaaaatca aagattctgt taagtttgaa aatgtaaaaa acttaagtga attatatata
    10081 acattaggta atgttgcttc atctgttgga aattcttctg cagctataaa aggtattgta
    10141 cttgatattc ctatattgaa agataataac tatattacgc agatacttta tatgaaagat
    10201 gggaaaatta acaaagcttt tagtgattat gataaaacta ttactaaatc ttattatata
    10261 cctgtagatg atattgacaa agataagatt atagaaatac ctatagttgc aggcagtaca
    10321 ggtaataata aaaatactta tagttctaaa tcatctgcaa ctataagttg gtatagatgg
    10381 aatggaaaag aaggtgctga ctcaagttta atatttacaa gtcagatata ttataattat
    10441 aaatataatt ttaaattatt tattccaaat aatttatttg ataaaatact tgttgaacag
    10501 gaatttgttg gagaaaaagc agtatttaaa ttttactatt ttgactattc agaccgtaag
    10561 aatttattta atatagtagt agagagtaaa aataaacttg aagatagaaa aaatacaggt
    10621 actcaaaatt ctattgtact tcaagagagc gatgaatata ctttcttact agttgtaaac
    10681 aatgcaaatg aaatggataa gctagatatg actatagacg cattaaaaga atatttctca
    10741 ttaatatatg gttaatatta tactcaattg gaggttttat tttatgaagg aaaagatact
    10801 tatactagaa gatgaaattg gtattagaag ttttgttagt ataaacttaa aaagggaagg
    10861 ctatgaaata gttgaggcag gtactggaag agaagctata gaaaaaatga caacagaaaa
    10921 ggatattaca atagctcttt tagatgtaat gcttccagat ataagtggta tagaagtatg
    10981 taaatttata agggagaatt ttgaccaagt aggtataata atgcttactg ctaaagctca
    11041 agaagatgat aaaatagaag gatttatctc aggagcagat gattacatca taaaaccatt
    11101 tagtataaaa gagcttttag tcagagtttc tgctcttctt agaagagtag caaaggatga
    11161 ttcaagtgtt aagtcgagtg agatagtttc tccaccattt atacttgata tagacaaaag
    11221 aaaattattt aaaaatggaa aggaaattga gcttacacca actgaatttt caatagtgaa
    11281 atatctaata tcaaatgcta agcagtcgtt aagtagagac cagatattag acgaagtatg
    11341 gggaactaac tatttatatg attttaaaat agtagatgta aatataagaa ggataagaaa
    11401 taaaatagaa gatgacccat caaaaccaaa atatatacaa actatttggg gttatggata
    11461 ctgttttaga aaggaagaat aatagtgtta cattttgaag gtctgagaaa aaaagtaata
    11521 aaaaactatt ttataataat tattataatg gttactctgt ttgaaggatt gttcatgttt
    11581 tacatacaaa actactatta tgattctgtc aaacagctac ttgaatctga aataaaatat
    11641 gctgatgaat ataatgctat tacaatggaa actacaagtt ttgagaaaaa agtaaaaaat
    11701 atatttgata aacaaccctt aactaaaaat tcagaatttg gaatttcaat aatagataaa
    11761 gataaaaata ttatattaga ccaatatggt tttaaaagta aagaaaaagc aaactatgaa
    11821 gatgttaaca atgcattaaa agatataaaa actaaaaatt taacacctta tacttataga
    11881 ataccagata caggggagca tgttatgagt atctccttac cacttaaggt aaataatata
    11941 atagaaggtg ttgttagata tacagtatca ttagatgcta ttgataatgc aatattaaag
    12001 caagctacat ggcttatatt agcaggaata tttatactta ttatagctat actaataagt
    12061 cttaaatttg ctgagactct tataaaacca ttacgagagc taaagaaatt tgcaaatgaa
    12121 ttggctgtag gaaattataa tataaaatta gaaaagatga agattgtaga tgatgaaatt
    12181 ggtgatttag cacagacatt tgaacacatg gcacatgaga ttgacaaaag tgaaaaatta
    12241 aaagaggaat ttatatcatc tgtatctcat gaactaagga ctcctcttac atctataaag
    12301 gggtggagtg aaaccttagg ttatgaaagt ataacaagag aagaactaga cttaggttta
    12361 ggcattatac aagatgaaac agaaagactt ataaaactag ttgaagaatt attagacttt
    12421 tcaaggcttt cttcagacag gataaaactt catgttgata tagttgatgt tgaaggttta
    12481 attgtaggtg ttgtaaatca acttaaagtt aaagcagctg aaaaagatat atcactttta
    12541 tttgaatttg aaaatgaatt tatagagaac attcaaggag ataaaaatag attaagacag
    12601 gttctcataa atttaataca aaattcattt aaatttacga gtcaaggtgg ttacataaaa
    12661 gttgtagctt ctcaagatga agagataaca accatatcag tagaggataa tggttcaggt
    12721 atagaaaagc aaaatttaaa taaagtgtta gataaatttt tccaagaaga ttataataag
    12781 gctggtagtg gattaggact agcaattagt aatgaaattg taaaacttca tggtggaaga
    12841 atgaaaatag aaagtgaaaa gaatgttgga acaaaaataa cttttaatat aaaaaataaa
    12901 tttgctaaac aagcataaat tcttagaaag aggattaaga tgagaaacat aaaaatgata
    12961 gtagcatacg atggttcaag atataaagga tatcaaaaat taggtgacaa taatatgact
    13021 attcaagaaa aacttgaaaa tgttttaagt aaaatgacta atgaaacagt ggagataata
    13081 ggttctggaa gaactgatat gggagctcat gcaagaggac aagtggtaaa ttttagaact
    13141 aattgtatgg attctttgga taaaattcaa aaatatttat atgaatattt accagaagat
    13201 atagttgtaa aaactgttga agaagttgat gaaagatttc attcaagata caatgtaaaa
    13261 tcaaaaactt atatgtataa aattgacaat aataagtatc atagtccttt tataagaaaa
    13321 tatgctactc atgtaagtaa aaaattagat ttagatagaa tgagaaaagc aagtgagtat
    13381 ttagtaggag aacatgattt taccagtttt gcttcttcta aatctaagaa aaaatcaaat
    13441 gtaagagaaa tttattctat aaatataaaa gaagatgata atgttataga aatatatgta
    13501 gaaggaaatg gatttttgta taacatggta aggataatag taggtgcttt aatagatgtt
    13561 ggtctaaaaa gaagagctcc acaagatatt aagtatatgc ttgaatcaaa agatagatgt
    13621 caatcatcag atactgcacc agctaaagga ctatgtcttt ggaaagtaac atattaatca
    13681 attatataaa actctcttta aaataaaaat aaaataaatt atatagttga cataaatgga
    13741 aaaataaact attatatatt ttataagaga gatataaaac tttatattta aatcctgtga
    13801 gaaagagagt aacttctatg tgttttatag agagctgagg atggtggaag cttggcaaat
    13861 aagtattagt gaaaagaact ttggaggata ctaattgaat ttagtatgtt agtaggtaat
    13921 ctcgccttaa gagaaagagt ggataagttt atcttatcaa ttagagtggt aacgcggata
    13981 tatttcgtct cttggttttt ccaagaggcg ttttttaata tatttaatta ttagtgttta
    14041 tctggtatgt tgaacattta ctaattgatt agctaagttt atcaataaga gtggtaccgc
    14101 ggatatttcg cctcttagta ttttaaaata ctaaggggtt tttttagtat taaaatgatt
    14161 ttatgtgagt ctaaattctc agagtcattc aatattaaga atttctataa aataaaaatt
    14221 ataatttaag attgggagga attaaaaatg aaggaaaaag tagtattagc atattcagga
    14281 ggacttgaca catcaatcat aataccttgg ttaaaggaaa attatgaaga tatagatgtt
    14341 atagctgttt gtggaaatgt aggtcaggaa gataaaatgg aagatgtata tgaaaaagct
    14401 ttacaaagtg gagcatcaaa ggcatatgtg gatgatataa gtgaagaatt tgtaactgaa
    14461 actatattta aagccgtaaa agcagaggct aaatatgaag gaaaatactt attaggaact
    14521 tctttagcaa gacctataat agctaaaaaa ttagttgaag ttgctcataa agaaggtgca
    14581 aaatatatat gccatggatg tacaggaaaa ggtaatgacc aagttagatt tgaagctaca
    14641 attgcagctc ttgacccgac tataaaagtt attgcacctt ggagaatttg ggatataaaa
    14701 tcaagagaag atgctataga ctatgctgaa aaacataata taaaggtaac agcaactaaa
    14761 gctaaaatat attcagtaga tgctaacctt tggcatgttt caacagaagg tggagatata
    14821 gagcacctag aaaatgaaca taaaaaagat gtttataaac aatgtgtaga cccagaagat
    14881 gcatgtgatg tagctgaata tgtagaagtt tactttgaaa aaggtgtacc taaaaaggta
    14941 aatggtgaag aattatctcc agttgcactg attcataagt taaatgagtt gggttgtaaa
    15001 catggaattg gtgttataga tatagttgag aatagacttg ttggtatgaa atcaagaggt
    15061 atatatgaga ctccaggagg aacaatcctt tatgaagcac ataatatttt agaaagtgct
    15121 actttagaca aagatacatt acatttcaaa caaatggtat cttataaata cggagaactt
    15181 atttacaatg gactatggta ttgtaaatta agagaatcta tagatgcatt tatggaacaa
    15241 actcaagata atgttacagg aacagtaaaa gttaaacttt acaaaggaaa tataaaacct
    15301 gctggaatat ttactgaaaa tgctctttat gatgaaggaa tatcttcttt tggaaatagt
    15361 gaactttatg accacaaaga tgcagaagga tttattaatt tattcacttt accattaaaa
    15421 atcagagcta tgaaagctgg aaaataataa aataaattat ttaagagtgt ataaaaacta
    15481 tttttaggtt tttatatgct ctttttttat ttgaatttat taacttatac agtaaatagt
    15541 ataaaaaaat atactatagt tccaaatggc ttaataatgt gaaaatataa gacctaaaat
    15601 ataaatttta taaatacaat agttattgac tctctcattg taagagggtt tatactgaat
    15661 ctaatgaaaa tttatagtga ggagatagat tatatgagaa aaataattga aattaaacat
    15721 cattcacatg attatgagtg tatgtggaat ggtatagaag atttatatat aaataaaacc
    15781 ggtgaatgcc ttccaaacga ttttttcttt ttactgtcag gttttggttc attttgttat
    15841 atgaaaacaa ataaagctga tttgaaaaga atggtagctt tgggagatgg aaggacaaaa
    15901 cagatgtata aatttttagc accaattgtt ggatttgaat acaaacatca tgagtttaaa
    15961 aagtatgagc aagcattaaa gaaagcaaaa tctgaaattg atgatggata tccagttatt
    16021 ttaggagctt tagatatgta ttatctacca tattataaga agctatatca taaagaacat
    16081 attccttttc attatatatt aatggtagga tatgatgatg aaggaatata tctgtatgat
    16141 tgtggtagaa cagaattatt acatctttct tatgataaat tacgtgaaag catgaattgc
    16201 aattcccctg gattaagcaa agaaaatact atttgcacaa taagaatgaa tacttcaaag
    16261 aataaatata acattgctag agaagctatt gctataaagc gtgatatgtt tttaaatcca
    16321 cctaaaggat ttttgggata taaaggtttt gaaaagttta ttaaagagtt gccacagtgg
    16381 aacactgaat tggataaaga tgattatgat aagattttga ccaatatggt tatgtttttt
    16441 ggaacagtac caacaattcc aaatgcaatc aaaggaatta aagaaccaga tactgttcaa
    16501 tttaaaggtg gatttgataa aatgagtaga atgctcaaca gtattgggga ggaatacaaa
    16561 aacgactctt ttatacaagt agctgatatt tttgataaaa ctgctattat aattgagaaa
    16621 atatccaata ttataataga ttacttaact caaacatgtg atgatacagg acaacttccg
    16681 ttattgttct ctgaggtctt agagcatatg aaaacaggat atttaatctt ggatgtataa
    16741 aaaatatatt gttgacccta acattacgta atagtgtaat atgaaaatat agcttgaata
    16801 tattagtagg tattgatggc tgtaaaatta aaatttgagg tgtggatatg agaacagtga
    16861 aacaagtttc tgatttaact ggaataagtg tgagggcatt acattattat gatgaaatag
    16921 gtcttttaaa accaaataaa attacagatg caggatatag actttatgat gatgaatcta
    16981 ttaaaactct tcaacaaatt ttatttttta aggagattga tattccttta agagaggtta
    17041 aggaaataat gtcaagccaa tattttgata aggttgaagc tctaaaaaat caaaaaaaat
    17101 tacttatttt aaaacgaaag agactggatg aattgataga gcttataaat caaactctaa
    17161 gaggagaagg aaatattgat tttaaagagt ttgatatgag cgagtatttt gatgttttag
    17221 aagaatttaa aagagagcat aggaataagg taattaaaat ttatggtagt gtagaaaaat
    17281 acaatgaata tatagaaaga gttaagtcta atgaagagaa aattgctaaa atggctataa
    17341 aacaatatgg aactattgag aagtttgcta aagctataaa aacaaatttt agcagtgata
    17401 tattgaattt aggagaaaaa tttgatagat ataaaaatga ttgtttaaaa gaaaaacatc
    17461 caaaattaaa agaattatat agaaagcttg tagaagattt aaataggaat ccttcttcaa
    17521 cagaccttca agaaatagca aaagagataa cagatatatc taaaaaagat tatgaaattt
    17581 tctctatgga tactggtgat gacaactggt attatatggt tcaaaattat ctagtaaatc
    17641 ctatgtggat agaagaagta gataaaaagt atggaagtgg agcaggtaag tttattggac
    17701 aagtactaaa gacttatcta agagatagaa aaccaaagat aaatacattg tatgaaaaac
    17761 ttgtagaaga tttgagtaga gattgttctt caagggaagt tcaaagcatt gttgaagaaa
    17821 tagacaatga aatgaaaaga agtaatgaat tttataaaat agacaatggt gaaagatact
    17881 ttgattatat gtcagagctt tatttacaag attctaacta tataaaagta actgataaga
    17941 attatggtga tggtgcatct aagtttattg gagaagcttt taagatttat tttgataata
    18001 ataattgttg aaaatttgga agtaaaaaat atgcaagttt tataaaagca caaaaataag
    18061 gatgtagatt agctccatct atatccttat ttaaagttat taaaatatat agtttacatc
    18121 aaacttaaca ctttcaataa tatttcccaa atatgttttt atactttcaa cttcttctaa
    18181 agattttaat ttattttcag tataaattgc tttgtccaga atattaaacc ttaatcctat
    18241 ttcatcaaag tactcatgat ttaaaacttt gtaccaatgg tttttttctt tattccaact
    18301 cgtagatatt gaatcagctt ctttttgagc tttttcccaa tcttcatttt ctatagattt
    18361 ttctaaaaca gatatattgt ctttatatcc ttctgtaaaa tcatatagtt tattgttaac
    18421 ataaaatcca aataatacaa ataagatagt ccatagtata acaaatgttg tagatttcaa
    18481 tattaatcac cctttttatt ttgaataaat attttgtcat tttcatctaa aacacaaata
    18541 agaacatctt taaaagtttc tatgtgatta gattttaaaa tacccattaa ccagttagta
    18601 tctttttgag ctatgtctag atttttattt attattctac catccagtat aagtgggata
    18661 ggtaaatgat taaaagctct tggaggagta ctattgtagc ttgatgctgg tacaacactt
    18721 agattaccat cagtctccaa aatagcatat tgtacatctt ttaggttaaa gtatccttga
    18781 atcctaagtt gttctaataa ttcatctata ctaattcttt cttttttaag ctctttatat
    18841 acaatttttc ctttatctat taatacagaa ggtttacctg ataaaaatga acttaatttt
    18901 ctacttttta atgataagaa tgaaattaga gtctgcatga ttacaagtcc agatattgct
    18961 gcaacaccat ttatgattgg tatattatta ttttccattg ggacagatgc aacttctgca
    19021 attaatagtg ttataactaa atcaaaacag ttcatttcag ctatttcacc ttttcccatg
    19081 actctaagtg caattagcac tgttatgtat aatataatac ttcttattaa tactactgtc
    19141 atatctatca attactcctt taaaaaatat ttacacttag ttagtatatc ctatataaaa
    19201 aatttaattc aaagtataaa aaaccttaaa taattggatt caactattta aggttttttt
    19261 gataatttta atattaaatt ttttatggat tttctgttac ttttatttat tttaataaca
    19321 gattcaacaa aactcttttt atctcctaat tttaagataa aatcaggata ttcttcaaat
    19381 tcattgagtt ttattaattt gaaagaagaa gatagatttt taaagttaat aattttagag
    19441 ttattcacta atacacttct ttttttaaaa taaatttctt ttttacttat atttagtata
    19501 gttaatattt gtttaagata tattattata tcttctggag ttctatcaaa tttgagttca
    19561 aaattttcat cttcaaagta gggaatataa tataaaggtg ttttgagagc aggtaattta
    19621 ttatataatt cttcaagaat atcgatattt ttaggggagt taaagaaaaa gcatttacat
    19681 acattcatta aatccacagc atctgaaagt gagttatgtt taatatctcc ttctacagaa
    19741 aaaatttttt tcatattcag taaagatatt tgcttagata agattctatt attgtatgta
    19801 atatgttgag atataaaagg ttgaatgtca atcatcatat taaataactc taagcattta
    19861 cctgtatatt tatatactct aaatgaagtt agaaggccag ttatatcaaa atcaccccaa
    19921 ttatatattg taacatcatc aaatttacaa aaccatttat aaaaatcatc cataacatat
    19981 aaaagattag gagcattatt gatatctagt tggttcaatt tggttaaatt tttacattta
    20041 tttgaaagta ttttattgta ttttggctta actaaagagt aataattatc tattgtatta
    20101 aattcgttat caactaaaac tccaccaata gaaattaatt ccgcaccatt atattctttt
    20161 ggtttatttt tattatcatc tagtatatta aattcaaagt ctataaataa cttgtatttc
    20221 ataaatctaa agcacatcct caggtaaatt atttattgag cctaagcatc ataagaatca
    20281 atagatataa tgactctaaa catcaaaggt tcattacaac ttttatttat ttcataattt
    20341 aattcaaata aatcttttaa gatataattt ataagtattc tttcccaacc catttttaaa
    20401 ttaactttag agtctattaa tatattttct atctttttaa atatttctat atttgctggt
    20461 attatactga gctcaatatt acttttaaac gggttagact gtaaatctgt tataagtata
    20521 ttttttaatt cctctgtaag ttcagaaatt aaactatcac ctatgtattt ttttatgatt
    20581 tcctctctaa tttcttttaa atttagcaat attagtacct gctacttaca tagtagcttt
    20641 tcctttcctt ttaatactat tattcatttt tgaatcttat ttagatatat tttaagatta
    20701 aaagtcttta gctatataat tttttaccca tttttaactt atattaacat attacaggct
    20761 tatatctcca ccctgtttac attgaggatg gagtatattg cctattttga taaaaatgat
    20821 aggttgctat ttgcctattc ctttttctga taatttttct aacatatctt tggtcatttt
    20881 atctaaatca taactaaact taaagttcca ttcttttact gcactactac tgtctagaga
    20941 gttaggccaa gaatcagcta tactttgtct tacagggtct acatcatatt ctatcacaaa
    21001 atcaggaata tattttttta tagaatcagc tatttcctca ggagaaaagc tcatagcagt
    21061 aatattaaaa gcatttctat ggatgagttt atcagctgga gcttcgatta aatccactat
    21121 tgattgaagt gcatcaggca tatacatcat atccattttt gtaccttctg ctatgtaaga
    21181 tttatatctc ttatttttta atgcctcata atatatatca actgcataat ctgtagttcc
    21241 tccaccagga ggcgtaacat aagaaataag acctggaaat ctaacacctc tagtgtctac
    21301 tccaaatttt gagtgataat aatcacatag taattctcca gaaactttag tcacaccata
    21361 catagtatta ggtctttgta atgtatcttg aggagtcata ttttttggag aattttcacc
    21421 aaaagcagca attgaactag gtgtaaaaaa tttacaatcc aattctttgg ctatttcaag
    21481 accatttatt aatccattca tatttaagtt ccaagcttct agaggtttac tttcagcaac
    21541 tgcagaaagt aaagatgcta gatgaattat agtatctaca tcatattttt ttgcaatttc
    21601 aaaaaaacga ttcttatctg taacatctaa tatctcaaat ataccagatt cacatactgg
    21661 attaccttct tttactctcc ttgaactagc gattacattt tgttctccat actcatttct
    21721 aagctttatt gttaattctg aacctatttg tcctaatgca cctgttataa gtattttttt
    21781 caattttaca acctcctaag ggtttaattt atgtagtact acttattttt attttagcaa
    21841 atcttttgtt taaatacaaa gaattaaaga taataatata cgacttaatt gaatgaagtg
    21901 cagtaagtgc ctaaaaattt aaagctaata tataatggta tattgtataa caataaaaga
    21961 gtagattaaa ttattatata tagattgtgt gactaagtca cagaaaatag agttaattta
    22021 atatataata aaagcatatc aagtaaagct ttaaattaaa gtaactttat aaataaaaag
    22081 taatataaat tgataaaatt ataaatttat ttatagtgaa tatgggttgg attttataga
    22141 tacaaaaata gtatatatga tatgtagacc attagcaggt ttaaagtagc aaaacatata
    22201 aaataaaatt tgaaaggaag tataaaaatg acaagaccat caatatatca tagccaagga
    22261 tacacttgtg cagaggcatt aataaagtct tataatgagg aacataacac agatatacca
    22321 atctcaatag gaagtggtat gggggttgga atgaatgttg gaagtgtttg tggagctgta
    22381 aatgctgctg ttgttataat agggtattta aatgggcgaa atagtaattt agatgaaaat
    22441 atagcaagga agtattcaag agagctattg aatagagtaa gaaataaata cagctcagaa
    22501 atatgtgtat ctcttaaaaa aaataaagta acttgtgaag aaataataaa tttcacatat
    22561 gatgctctaa atgatatgct aaaaaatcaa aaataattct aagaaacaag tattttacat
    22621 tcgatagaat acttgttttt ttatgatatt tttaataagt attagtttat tataattttt
    22681 aaatcaattt tattatattt taattgaaac aatctacttt gaaaattact aactgacaaa
    22741 agaatatgat tatagtatag aaaaaatgta aattatttaa tattaagtga gagtatatgt
    22801 ttacctatat aaaatcgtat aaattaatgg ctaaatttat aaaatataaa gaaatatata
    22861 ggaaaacaaa agaattaatt agtataatat aaagtttaaa agttaaaatt tatataattt
    22921 atttataaag taaagaaaaa atagaaattt gtaataaaat aaaaagttta tgacataata
    22981 tggtataatt agtaaaaata tagagattgg aggaattgct ttgtttatag atgaagaatt
    23041 agaaggttat atattaacat gtaaaatatc tgaagacttt aaaaatatac ctgaatatag
    23101 tgatgaagag ttttatgtta cagtctataa agatgaaagt tctgactctg ggtactatgc
    23161 tttattagaa aataaagaag aaagagttgt atgggatgga gaagttgttg ccaataatat
    23221 ttttaataac ctttggattg tagtaaataa ggttaaaact ggataatagt ctaattatat
    23281 agtatttgta atattttaaa tgaattttta catatttgag atgagtaaat aacaaagatt
    23341 attcgatact ggaggagtaa ttcaatggaa tatagttata gtaagatgaa cttaaaaaaa
    23401 ggggatattg tagaggtaaa tttagagaaa caagccaatg taatattgtt agaccatata
    23461 aattatgtga aatttaaaaa tcaaagaaat tatgattact atggtggatt tgcaaagaaa
    23521 aatccatgta gaatgagagt tcctaatact ggaacatggt atctagtagt aaatcaagat
    23581 ggaaatagtg gaatagtaaa cttttcaatc aatacaattc aaaattaatt ttagaatatt
    23641 ggatattctt aatttttgta cattttatat agttaaaata aaattaataa gcaattatta
    23701 taaaataatg tgggtgcctc aaaatgaatt ttataattca tgagacactc ttttttatat
    23761 gaactcaaat atactttatc atttcaacaa tgaaaaagag gcgtatctgt gttttgagat
    23821 agcctcattt atcatatatc atatatagaa ttttaattat tcttaactaa aattttttcc
    23881 ataggttatc tgtacaatta aaatatttat tgttagcata cagctccacc tgtaaggtgt
    23941 aaaagagcct ctagtatctc ttcttttctt tccaaaactt catctgataa aagcatttct
    24001 ataaatttat cagcaccgat tctatcgata gttattccaa aacgttcacc tgtgttaccc
    24061 tgttctctaa ataaaagaat tgctttttca attaaagtca taagttcctc ttttgaaaat
    24121 aatctatcaa tctgtgttcc aggacgaact gattttcccc atttccctcc tatatagact
    24181 ttataaccag attctttttc ttcaatacta tcaaaattac aactttctat acacttacca
    24241 cagttattac atagattact atctatttca agtttacctt tatctgttaa tttagctgct
    24301 ttaacaggac agacctctac aacagcacat tttttacaac caacacaaag ctctgaatca
    24361 tagtcaggta cacgttgacc aacaattcca agatcgttta ggtcaggttt aatacagtta
    24421 ttagggcatc caccaacacc aattttgaac ttatgaggta gttttacatc ataccaattt
    24481 ttgtaaaatt cttcatgtat ttcagcagca agtgcctgtg tattagctag accatgaaca
    24541 caaacagtac ctttacaagc cacaacagga cgaacccttg atccagttcc acctgttatt
    24601 agattttcgc tagcaataaa ttcacttaga gcttcaatat cttcaaattt tacaccaggt
    24661 aattctactg ttagtctagt agtaaatgct aactgaccgt ttccgaattt ttcagcagct
    24721 tctgcaattt tttttgcttg tgatgcattt attacaccat ttacagttat tatacgtgct
    24781 gcaaaatgtt ctccatccct gcttggtaga aaacctctac cttttacttc ctttttttgt
    24841 tctgctgtaa cattactcat atttactcct ccattacctt aagatttgga taaaatagta
    24901 ttccaccttc aagaactttc gtatttgtat agccaaagcg tcttaagcga ttttgcgcca
    24961 tatagctatt tttaccttta gcacatataa gaagaatttt ttcatcctta gcaagacctt
    25021 caatttctcc attaatttca ccaacaggaa tataacgtaa actaggaata gaaggagctt
    25081 ttgaaacatc aagtttagtc caactgtcaa aatcctcaat ttcgtcaagt aaaacactat
    25141 caagttctcc attaagttta ttttgtaaaa catttactgc cactacaaaa gggtgaatag
    25201 ctgttgaaaa aggtggtgca tatgcaaggt ccatactttc taatgaataa aggtcagcgc
    25261 ctaaagttat tgcagtagca gcgatatcta ctattttatc aaccgctcca ggtcccataa
    25321 cttgaacacc taataatttt ctagtatttt tttcagcaac cattcttata ataaaattag
    25381 atgcacctgg ataataatga gctttatcat cagtagctat ggttacagag caaacattat
    25441 aaccttcttt ttcagctatt tctttactta gacctgtttt tcctgcattg agctcaggta
    25501 attttactac tgttgttcca agaacaccat tgtaagtttt agattttcca gatatatttt
    25561 gtgcgcaaat tctaccctca tgatttgcag atgaccccat aggtgaccat gtaggtttat
    25621 ttgttaaaga attttttaca aaagcacagt ctccaactac atatatatct ttgtcatttg
    25681 taagcatata ttcatctgca ataattgtat tattaggggc aaattctaaa cctgtgtctt
    25741 ttagaaaacc tgtgttagga cggattccaa ctgacataac cactaaatct gttttaatag
    25801 ctctattttt agtacgaagt ttttcaactt tattttcacc ttcaatacct acaacctggt
    25861 cacctgtaaa aatcattatg cctttatcag ccatatgatt ttcaacgtat tcagcaaaat
    25921 cagtatcaaa tccaggcata acatgttcag ccatatcaac aacacttaca cgtataccca
    25981 ttgcagaaag attttcagca atttcaagac caatgaaacc tccaccaata acaacagctc
    26041 tcttaatacc gttttcaata gaatctctta gttttatagc atcatttgga gtacgcataa
    26101 agaatactcc aggtaaatct attccaggta ttggtggttt tattgggtct gcccctgtag
    26161 caattacaag tttgtcatag cttagatttt ctttttctcc tgttgaaagc gttttcaaag
    26221 ttacttgttt tgcatttcta tcaatagatg taacttcaac tccacaacga acttctgctc
    26281 ctgttagttt agaaaaactt tcaggtgtat ttacgattaa agatgattta tcttcaatta
    26341 tcttaccaac gtaatatgga agaccacaac ctgcataaga aatatcatca cctttattta
    26401 gtattgtaac agagcaatta tcacccattt cacgttttat ttttgcagca gtttttgttc
    26461 cagcagcaac tccacctata attaatactt tcacaatata tcacactcca atctacttta
    26521 taactctata ataaaattat acttcttgac aatatatttg taaaatacta aaatattaat
    26581 aaaatcataa gcatttacta atgtttgcta aggttgtgat aatatgacaa ttcgtagtct
    26641 tgaaatcttt gttaaggttg ctgaatgtgg taaaatgagt gaagtagcaa gaaatatgta
    26701 tattacccag tcatcagtta gccaagctat ttcagaaata gaaaaagagt atggtgttaa
    26761 gctatttgat agaatttcaa aaaaattata tttaactgag gcagggaaaa aacttcttgg
    26821 atatgggagg catcttttag ctgtaaatga agaaatgaat gattgtatga agcattgtgc
    26881 aaaaaatatc cgtataagag ttggggcaac agtaactgtt ggtacatgtg taataagtcc
    26941 aataatgtta gaattataca aggtaaatcc tcttatagaa cctgaagtat ttgtagaaga
    27001 tacaagactg attgcaaaaa aattacttaa tagtgaatta gatattgcga ttgtagaagg
    27061 aaaaattaag cacccagata ttgttacaaa aagcattatt aatgataatc ttgtacttat
    27121 atgttcacac aagcatgagt tttataaaag agactctata aaagtttcag aactttcaaa
    27181 tcaaccatta attatgcgtg aattgggaag tggtacaaga gcacaattag aaaagcaatt
    27241 aaaagaatta aaaatcccaa tgaatattag atggtcatgt tataactctg aagctatatt
    27301 aagagcagtt gtagataatt ttggcattgc agttatatcc gaattactta ttgaggatta
    27361 tttaaaaaag catcttttgt gggcttgtga tattgaaggt attaatttac ataggacatt
    27421 tgatattgca tatcatagaa gcaagttttt tacagaaaat atttcagcat tttttgatat
    27481 ttcagtagaa tatgggaaaa aacaagcgag aaagaagagt agaagtgtaa acagttt
//
LOCUS       DAYAOX010000029        27377 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.029, whole genome shotgun sequence.
ACCESSION   DAYAOX010000029 DAYAOX010000000
VERSION     DAYAOX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27377
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.029"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(182..1219)
                     /locus_tag="ACGI57_001006"
     CDS             complement(182..1219)
                     /locus_tag="ACGI57_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFG9716092.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(1238..2023)
                     /gene="etfB"
                     /locus_tag="ACGI57_001007"
     CDS             complement(1238..2023)
                     /gene="etfB"
                     /locus_tag="ACGI57_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HFG9716093.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(2056..3189)
                     /gene="acdB"
                     /locus_tag="ACGI57_001008"
     CDS             complement(2056..3189)
                     /gene="acdB"
                     /locus_tag="ACGI57_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative isocaproyl-CoA dehydrogenase AcdB"
                     /protein_id="HFG9716094.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(3237..4364)
                     /gene="hadC"
                     /locus_tag="ACGI57_001009"
     CDS             complement(3237..4364)
                     /gene="hadC"
                     /locus_tag="ACGI57_001009"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HFG9716095.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(4364..5590)
                     /gene="hadB"
                     /locus_tag="ACGI57_001010"
     CDS             complement(4364..5590)
                     /gene="hadB"
                     /locus_tag="ACGI57_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HFG9716096.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(5605..6405)
                     /gene="hadI"
                     /locus_tag="ACGI57_001011"
     CDS             complement(5605..6405)
                     /gene="hadI"
                     /locus_tag="ACGI57_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HFG9716097.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(6444..7643)
                     /gene="hadA"
                     /locus_tag="ACGI57_001012"
     CDS             complement(6444..7643)
                     /gene="hadA"
                     /locus_tag="ACGI57_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HFG9716098.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            8320..9318
                     /locus_tag="ACGI57_001013"
     CDS             8320..9318
                     /locus_tag="ACGI57_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFG9716099.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            9680..10207
                     /locus_tag="ACGI57_001014"
     CDS             9680..10207
                     /locus_tag="ACGI57_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HFG9716100.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            10234..10830
                     /locus_tag="ACGI57_001015"
     CDS             10234..10830
                     /locus_tag="ACGI57_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HFG9716101.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(10895..12478)
                     /gene="asnB"
                     /locus_tag="ACGI57_001016"
     CDS             complement(10895..12478)
                     /gene="asnB"
                     /locus_tag="ACGI57_001016"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFG9716102.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(13044..13880)
                     /locus_tag="ACGI57_001017"
     CDS             complement(13044..13880)
                     /locus_tag="ACGI57_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9716103.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(13904..15370)
                     /locus_tag="ACGI57_001018"
     CDS             complement(13904..15370)
                     /locus_tag="ACGI57_001018"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9716104.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(15373..17235)
                     /locus_tag="ACGI57_001019"
     CDS             complement(15373..17235)
                     /locus_tag="ACGI57_001019"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9716105.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(17482..18345)
                     /locus_tag="ACGI57_001020"
     CDS             complement(17482..18345)
                     /locus_tag="ACGI57_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFG9716106.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(18400..19332)
                     /locus_tag="ACGI57_001021"
     CDS             complement(18400..19332)
                     /locus_tag="ACGI57_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFG9716107.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNQKDLPIW"
     gene            complement(19418..19969)
                     /locus_tag="ACGI57_001022"
     CDS             complement(19418..19969)
                     /locus_tag="ACGI57_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9716108.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(20198..20689)
                     /locus_tag="ACGI57_001023"
     CDS             complement(20198..20689)
                     /locus_tag="ACGI57_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716109.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(20717..21277)
                     /locus_tag="ACGI57_001024"
     CDS             complement(20717..21277)
                     /locus_tag="ACGI57_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFG9716110.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(21429..22586)
                     /locus_tag="ACGI57_001025"
     CDS             complement(21429..22586)
                     /locus_tag="ACGI57_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFG9716111.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(22623..23564)
                     /locus_tag="ACGI57_001026"
     CDS             complement(22623..23564)
                     /locus_tag="ACGI57_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716112.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            complement(23651..24343)
                     /locus_tag="ACGI57_001027"
     CDS             complement(23651..24343)
                     /locus_tag="ACGI57_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9716113.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(24452..25264)
                     /locus_tag="ACGI57_001028"
     CDS             complement(24452..25264)
                     /locus_tag="ACGI57_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFG9716114.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(25315..26226)
                     /locus_tag="ACGI57_001029"
     CDS             complement(25315..26226)
                     /locus_tag="ACGI57_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9716115.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(26285..27016)
                     /locus_tag="ACGI57_001030"
     CDS             complement(26285..27016)
                     /locus_tag="ACGI57_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFG9716116.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            27105..27356
                     /locus_tag="ACGI57_001031"
     CDS             27105..27356
                     /locus_tag="ACGI57_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716117.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
BASE COUNT         8792 a         4896 c         3056 g        10633 t
ORIGIN      
        1 aatttgtaag caatatacat ataaggatta tccagattat cttgtattat aaaattttat
       61 tcaaacagac tttttaataa ctgacaaata cacactagca ataacttatc aaagggttaa
      121 taattatgtt actaactaat aagacttatt caatcagtaa tatttatata gtttgtatct
      181 attaagcttt tatagcttct aataaatcat caacgctatc taatttttct ataagcattg
      241 gaactatttc atgtaagtct ccaactactc catagtcagc gatttctaaa attggagctg
      301 ctgggttttt gtttatagca attatgaaat ctgatgattg catacctgct aaatgttgta
      361 ttgctcctga tataccacaa gctatatata ggtttggttt aacagtagtt cctgtttgac
      421 ctacttggtg agaatggtct atccatccag catcaacagc cgcacgagaa gaaccaacta
      481 ctccacctaa tttgtcagct aatttcttaa gcatttcaaa tccttctggt ccacctaatc
      541 ctagaccacc tgatactata acatttgcat ctgtaagaga tactaaatct ttcttagttt
      601 taactgtttc taaaacagta gttcttatat catcttgagt tattttgtag tctaatgcta
      661 taacttcacc agttcttgtt tcgtcttttt cagctttatc cataacacca ggtctaactg
      721 tagacatttg aggtctatgg tttggacaaa tgattgtagc cattatgttt ccaccaaatg
      781 ctggacgagt ttgttttatt ttcttatctt ctgggtctat ttctaactta gtacagtcag
      841 ctgttaatcc agttccaact cttgaagcta ttctaggtgc taagtctcta cctatatgag
      901 ttgctccgaa aagcattatt tctggtttta tttcatctat tgcatctttt attacttttg
      961 tatatgcatc agttgtatat ttttctaaaa gagcatcatc tgcaacataa actttgtcag
     1021 ctccaaaagt gattaattct ttagctaatc catcaacatc ttttcctaat aatattgcac
     1081 aaagttctgc atctacttct ttagctaatt ttcttccttc tcctaataat tctataacta
     1141 ctggagctat ttttccttct ctttgttctg caaatatcca tacgttttta taagaactta
     1201 aatcttttat atcattcata gtttgtactc ctccaactta tataatgtgc ttttgtttta
     1261 gaccgattat tattttacta gctatttcat cagctttacc ttctaagatt tctccttttc
     1321 ctcttggttc aggagtaaat gatctaaata ctttagttgg tgatgctttt aaaccaactt
     1381 cttctttatt tacatctaaa tcatcaattg tccaaacttt tacatcatgt ttatatgctt
     1441 ttactatttt atcaacagac atgtatctag gtgtatttaa ctcttttact gctgttaaaa
     1501 gtacaggtgt acttactttt attaattcgt atccatcttc taattgtctt tgaactatta
     1561 tatcttttcc ttctattttg aaatcttgta catatgtaac ttgaggaatg tctaatttct
     1621 ctgcaatttg tggtccaact tgtgcagtat ctccatctat tgcttgtctt cctgcaaata
     1681 ttatgtcata gtcacctact ttagatattc ccgctgcaat agtatttgat gtagcccaag
     1741 tatcagctcc tccaaatgct ctatcactta ataatattgc atcatcagca cccatagcta
     1801 aacactctct tagcataaag tctgcttgag gaggtcccat tgttataact gttatagtta
     1861 catcatcatg ctcatcttta atttttaaag cttcctctaa tgcatttgca tcatctgggt
     1921 ttaatatact tggaactcca tctcttataa gagtacctgt ttctttattt attcttactt
     1981 cgttagtatc tggaacttgt tttacgcaaa ctaatatttt caaagtctat acctccaatt
     2041 tcattagatt atttattatt ttaatacgtt tgatgaaatt accatttttt gaacttctga
     2101 tgttccttcg tatattgata cgattctagc atctctgtac attctttcga tttcataatc
     2161 ttttataaat ccatatccac cgtgaagttg taatgcttta taagcaactt catttgcaat
     2221 ttctgatgca tagtatttag ccatagcaga ttcttgagca actctcattc ctgcatctct
     2281 cttttgtgct gcatcataaa ctaatcctct agctgcacaa acttttgttt ccatgtcagc
     2341 tatagtaaat tgagtatttt ggaatttagc tataggctta ccaaattgaa ctctttgttt
     2401 tgtatattta acagcttcat ctaaagcacc ttgagctatt cctagagcta aggcagcaac
     2461 acctattcta ccaacttcta gagtacccat tgctatctta aatccttgac cttcttttcc
     2521 aagtaagttt tcttttggaa ctttaacgtt ttcaaatatt aagtcagaag tttcagttcc
     2581 tcttataccc atcttatctt catgagctcc tgttgagaaa ccatcccatt tactttctac
     2641 tataaacgct gatgttcctc taagtccttt acttctatct gtaactgcta ttactatagc
     2701 tacatcacag aatggtccat tagttatgaa agtttttcta ccatttaata tatagtggtc
     2761 tccaactaat tctgctgttg tttgttgtcc acctgcatca gaaccagctc ctggctcagt
     2821 taaaccaaat gctcctatta attcacctgt tgctatacct tttaagtatt tttgtttttg
     2881 ctcttcagta ccattgtaaa gtattggtaa tgaagcaaga gaagaaccag ctgtaacaaa
     2941 agtagctgtt gaagcacatc ttctagctat ttcttccatt actatagcgt aagaaacata
     3001 tcctgctccc ccaccaccat attcttctgg gatattactg cttataaatt ttgcatcagc
     3061 taattttttg attaattcct taggcattac acctgtttta tccatttctg ctggtaaagt
     3121 atctaattct ttactaacaa aatctcttac tgcttttctt aaaagttctt gttctttgtt
     3181 atataacata gcttcctcca attttcatat tttatagtaa atttatttta atgaacttac
     3241 gctaaactca tcatctcagc aaaagtttga atacgagttc tggcttgttc attattctga
     3301 gtttgttggt cgatttcaac atataaagta ggtattccac tatcttctat atcttttcta
     3361 actaaaggat aatcgtattc ttctgggtca cagaatttca tcatacagaa gataacacca
     3421 tctatatctt tctttttaac ttcatctact ataagtgacc cacgtttttt cttagggtca
     3481 taagctaatg aacatccttc tatgtttgac cattgtcttg ctaatctttc taacgcatca
     3541 tcacctgctg gtacatctgt tctgaattgt cttgtttctt gtgctaagtc gtcagctaca
     3601 actgatatat tgttgtcttc taaaatgtct aatatatctt tagaatcagc taatatacct
     3661 gttaataaaa ctttctttcc agaacagact tcttctggca tagcatttaa ttttgctatt
     3721 aaatctttca ctagctcagt atgttcttct tttctcataa agaacccact cttaattact
     3781 aagcttctta ttgatggttt tatagtatta gaatgtttat aagctacttc aacaaagtct
     3841 ctcatagttt ttctatgttc attgtaaact tctatacttt catgaatttt tgcttcttct
     3901 atttcatatc cacaaatttc ttcaagttct ctttttacgc ctttgtactc actgattaag
     3961 tattttacac cagcttcaag ttttctattt tgtgggtgta ctaatgatat atattttata
     4021 tgaggtactg ctgatttcca gttttgtcct aaacaaatta gtgtatcaca catacctggt
     4081 ataataactc cagataattc atcataagca ccttttaatc catattctaa acatgattgc
     4141 attattgaac atgcaaatgc agggaaatat tgtttagcta aatctaattc tgtttgccct
     4201 ccccatatac caactggaag cattccagct gcatgtataa tttcttctgg gcaataaact
     4261 gggaaacaac ctatagcttt tttaccagtt tcacttttat atttttttac agccgcattt
     4321 ggattttcaa ctacttcttt cattttagat aaaatagctt ccattatatc tcacctctat
     4381 taagtttttt tctttcttcc attacttcaa ctaaaccttg aattcttgtt tcaaattgag
     4441 cattagtgaa agctctaggg tctgcttggt caccatcaaa tccagcatat ggtaatccag
     4501 tttcttcagc tgctcttctt tgcatttcat attgtattaa actcataagt ttacagcttc
     4561 tgttcatatg atagaatgct ccatcacatt taccctctac taaagaatca actctatatt
     4621 ttgtcatacg gtctaggttt acattgttaa acatagtact atatgctact gccattccat
     4681 ctaaatcatt aacttcatat tgtaatgccc aagcatgtgg gtaaacacta cctgtcatgt
     4741 taactccaaa tttagctaat gttttcatct tgtatcctat atatggccaa caaggtatac
     4801 cttccatcat tattctgtat ttttcttccc ctctgaaaga agatttacca gttttcatgt
     4861 tgtcctctaa ttcttctata agtaacttaa atgcttctgt agtttctttt ttacctctag
     4921 cacaaactat tacagccatg taagtaaata agtcaaatcc attcattgga gaaggagaag
     4981 aatctgctgg taaactcata gaatacttcc atagtcttcc gttttcagct gatattttca
     5041 ttacttcttc aaacttctta gggtcaaatt tctttcctga tataatttct agttgtttta
     5101 tagcttcttc aaattgagct ttaatataat ctattcttga ttgagtaact tcgtcttcat
     5161 tattgaaagt tgtatcaatc attattaaag gtatatctaa ttctcttgaa atattttcat
     5221 accattttat aacttggtta catatattgt tacagcaaag taggaaatct ggagctggca
     5281 tatccaaagc ttcacatcca ccattttcta aaagaccaaa atttgttctt gcatatgcac
     5341 ataggtcaat agaatatcct ttagattcag ctatttcaca taattctaaa gaaccttttt
     5401 tagctgctac tccagctgct tggttttctg gatataatac gtttaagtca aatacttctg
     5461 ctaactcttg aggaaatact gatgttgacc aacctacagg tcttccttct tctttagctt
     5521 taaatgcatt tgcatattgt tcagctaata aatcattaat tactactcta gcttcttttt
     5581 tttcagacat tttcatccct ctttttatat atttttcact tctttttgtg attcttttgc
     5641 ttcatcaaaa gcatataacg ctgctcctaa agcaccagtt aattgaggta tatcaggtac
     5701 aataatttct gtgttgattt ctcttgccat agctcttaca ataccactat ttctagcaac
     5761 tccaccaacc ataactacat ttctttgtac tcctattctt tttactaggc tagaaactct
     5821 ctttgctact gaagtatgaa tacctgccac tatatcttca atttttgcat tttcagataa
     5881 atgtgatata acctcagact ctgcaaatac tgtacatgta ctgcttattg atacttcatt
     5941 ttgagaattc atagatatac ttccgagttc agatacatca acctctatta tttttgccat
     6001 tacatctaaa aatcttcctg tacctgcagc acatttgtca ttcataagaa agtttaatag
     6061 tcttccatta ttatctaatt ttaatacctt tgcatcttga ccacctatgt caataatggt
     6121 tcttgtctct ggaattatga aatttacccc tctagcatga cagcttaatt cacttatttg
     6181 cttatcagca tctgaatagt tcattcttcc atatcctgta actacaactt ttttaatatc
     6241 ttctcttgca agacctgtct tgccatataa tttttctaaa actcttgatg gtccagtagt
     6301 cccagtacca gaggatattg tttcagaagc tacaatatct tccccattct ttaagattac
     6361 tccctttgat gcagttgaac ctatatctaa tcccattgtg tacatcttta gcattctcct
     6421 tttaaaaaat ctagaattac taattaatat cttacaactt tactatcttt aaagttattt
     6481 attttctctt catcgtagcc taaagatttt aatacttcta cagtatgttg acctactttt
     6541 ggtgctggtg tatattcttt aattccttca tttctaaaca taactggagt attaactaag
     6601 acacctgtat ttccgctatc gtatgttttc ttaaataaga agtcatttgc ccaagcttgt
     6661 tcgtcatcta ataaatcttc acagctttga attttttcaa atggtaagtc tgcttcttct
     6721 aataaagctg accactcgtc taatgttttt tctaacatag cttctccaac tatcttaact
     6781 aaatcttcaa catgattaac cattgaatct atgttattat atctatcatc ttctaatata
     6841 tattctctat ttataacctt acagaatttg cctaaccact tgttgtattg tattaaagct
     6901 aattgaatcc atcttccatc tttacattta tatgtagtca ttaatgggct gtttggattt
     6961 tctcttgata aaggcatttc atttccgtat tgtgctgttg ttatcattgt tcccattcca
     7021 tatatagctg tatggaaaag acttactgtt actctctcac ctttaccagt ttgagctttt
     7081 ttatgtaatg ctgctaaact tcctgctgct agtgctagac ctgcatagtg gtcaccaaat
     7141 cctgctgctg tatttgctgg agatgttcct ttttccataa cagattggct aactcctcct
     7201 cttgcgaagt atgcagtata gtcaaatcct ggtttatctt ttaaaggtcc tttttcacca
     7261 tatcctaata tttgagagaa tattaatcct ggatacttat cttttatttg gtcataagct
     7321 atacccattt tttctaatgc ttgaactcta acattagtta caaatatgtc tgcttctgat
     7381 aataatttat gtaatatttc tactccttct tttgatttta cattaatact tacacccttt
     7441 ttatttccat tttctaattc aaacataggg ttttcatcat ctgatgctgg agatttaaat
     7501 gttccaccca taactcttat tccatcacct tctataggtt caatcttaat aacctctgca
     7561 ccccagtcac ctaacatttt tgcacaacat ggtgctgcga tgaaacttga aagttctact
     7621 actttaactc cttctaaaag cataacgttt tcctccttaa tagtatcaat tataaaattt
     7681 cgattaaatc actcataaaa tacttttttc tgatattaac tgtctgatgt caaagttcct
     7741 aatcttatct aaaataactt aaaaattatt aaaattattt tttattgttt tattgtctta
     7801 cacctatatt gttaagcatt tatcgtgcca actttatatt taatcaaaat atcgcttgat
     7861 ttgaatgttt tttaacaata tgcaattgaa taatcttttt catatcgtgg catcttgcca
     7921 ctatccattg cattttgcta ttctttctat gcttataaaa gtttttttta tatatttttt
     7981 tatgaaaaag cttgacattt caaaaaaagt tttcattgct aaactcaaaa acacatgtta
     8041 tcattgtaat taatcagcaa gttagttaac taatttcaaa cttagataat tttttaataa
     8101 tatttaatct aaaaaataac aatttctttg ataaatttta gagtaattat tacattaaat
     8161 aaaaaattac taaatatcaa aataacacag ggaaaacaat ttcaaaacta aatttttaac
     8221 cttaaaaatt ttgtataaaa aatataacct tatatgctac atattatttc taaatgaaaa
     8281 attaccataa taatttaata attacaggag ggtattgata tgaaaatact agtatttgga
     8341 gcacgcgatt atgaagaacc agtaataaaa aaatggtctg aagaacataa ggatgttcaa
     8401 gtggatattt atcctgaaaa catgactgaa gaaaatgtag ttaaagctaa agggtatgat
     8461 ggtatatcta tacaacaaac taactatata gataatcctt atatttatga aactttaaaa
     8521 gatgctgggg ttaaagttat agcttcaaga actgcagggg ttgacatgat acattttgat
     8581 ttagttaatg aaaatggact tatcgttaca aacgttcctt cttattcacc taatgcaata
     8641 gctgaattag ctgttactca agctatgaac cttttaagaa agactcctct agtaaagaaa
     8701 aaagtctgtg aaggtgatta ccgttggata gctgaacttc ttggaacaga agttagatct
     8761 attacagttg gtgttatagg tacaggaaaa ataggtgcta cttctgcaaa attattcaaa
     8821 ggcctaggag ctaatgtaat tgcatttgac caatatccaa atagtgattt aaatgatata
     8881 ttaacttaca aagattcttt agaagacctt ctaaaagaag ctgaccttat aacattacat
     8941 actcctttac ttgaaggaac aaaacatatg ataaataaag atactctagc tataatgaag
     9001 gatggagctt acatagtaaa tactggccgt ggtggtttaa ttaatacagg ggatttaata
     9061 gaagcactag agtcaggaaa aattagagct gctgcccttg atacatttga aactgaagga
     9121 ttgttcttaa acaaaaaaat gaatcctgga gaattaactg acccagaaat aaataaactt
     9181 ctttctatgg aacaagttat attcactcat caccttggtt tcttcactag tacagcgatt
     9241 gaaaatatag tttattctag tttaagtagt gctgtagaag ttataaaaac aggaactgct
     9301 actaatagag taaattagta ttttataaat atattttttc attaattagc tattatctaa
     9361 caaaatagcc tttaatttcc caaatataag agagtcagct cagtatatat tgagtagact
     9421 ctcttatatt attttttata ttaaacttgt actcttctta ttcataccaa attaaattta
     9481 tattaactta ttctaactac tcctactcta tacattttac ctatttttac ttttctaatt
     9541 atatacattt gaatgggtac aatctgtatt ttacaaaaaa aacacctaac aaactatgaa
     9601 aattgtatga ttaatattaa tatatttata agaaaacata acaattatgt tagctctgga
     9661 tttttaggga gggagtatat tggaaaaaat gaataaagtt acttctaaaa aaactaccta
     9721 tattgttact tcagcattat ttgcatccat aatatgcctg acaatagcat atatattaca
     9781 tattccagtt ggtggaaaca atggatatgt tcatatagga gatgctttta tttatctagc
     9841 tgcaactatt ttgccaacaa actatgctat agctgcttct gcaattggtg ctggtctagc
     9901 tgatttatct acaggtgctg ctatctgggt aataccaact ataatcataa aaccaatact
     9961 tgtattattt ttcacttcta aaagtgacaa gataataaat aagagaaata tagttgcttc
    10021 ggttgtagca ggtatagtag gattagtttt atacatgttt gctgaaggta taattattgg
    10081 aagttttaca agtgcatttg taatgagctt acttggctta ttacagccaa taggaagttt
    10141 tattgttttt ataatcttag gtatggcact tgataaactt gattttaaaa aacgttattt
    10201 taattaagaa tttaaattta tggagggaaa attatgaata tcttatatac aattgaaaat
    10261 agtatctatg taaatataac taatacatgt ccatgtagtt gtgttttttg tataagaaac
    10321 gaaaaagatg aagttgcaaa tagtggtagt ttatggttgg agcatgagcc aagtgtagat
    10381 gaagtaaaag aagccttcaa taaatacaat ttagacgact atgatgaaat cgtattttgt
    10441 ggatatggcg aaccattaat gagaataaat gaattgatag agatagcaaa gtttataaaa
    10501 gaaaaaagta gcataaagat aagaataaat acaaatggtc taagtgattt aatacataac
    10561 aaaaaaacag ctattctgtt aaaaaatgtt atagatgcag tatcaataag tcttaatgcc
    10621 ccaaataaag aagcttataa tagagttact caaccaaagt ttggagaaaa atcatttgat
    10681 tatatgttag attttgcaag agattgcaaa aaatatataa aagaagtagc tttttcagta
    10741 gtagatgaaa tatctccaga agaaatagaa gaatctaaac aactggcaaa aaaacttgat
    10801 ataccattaa gagttagaca taagaattag caagtaaaat tataaaacat taagaatcac
    10861 ctgaatggta tttcaggtga ttcttcctct tgtattaaca ttttacaaaa tagatttcat
    10921 accacactag gaatgtgtaa atcatccaaa tctttctcca attatcagag tttccactaa
    10981 tgtagtcatt aaaaatctca ttaatctcat ctatgttaaa gaatttatct gctatctcac
    11041 tattgaattt atctcggata tccttattat atcggtcatc agccatccat atacgaattg
    11101 gcacaataaa tcctaattta tttctaaaag caatctcttc tggtaatgtt tttgatgcag
    11161 ctgtacgaaa ggcaatttta ttctcttttc ctttcacttt atattctgat ggtattctgg
    11221 atgcaatatc aaatattctt gtatctgtaa gaggcattct gacttccaat cctactgcag
    11281 tactcatttt atctatatta aaataaatat ctccttccaa ccatatttga atatctacat
    11341 ttgacatgtt agtaagtgta tctagttctt ctgtttcttc ataaattcct tttaccactt
    11401 caataggaag cacatcagga ttgtatttct taagaatttt tcttttctca ccctcattca
    11461 taatgtttgt atttccaata taaaatgtct tcaaattctc tccataacac tctgccttat
    11521 ggtatatatt atatccacca aaaaactcat ctgctccttc tccagagtaa cagatatttg
    11581 tgttttcagc tgctgccttg catccaatag caaaaacgat agcagatgca tctccagttg
    11641 gttgctccat atggtacatt acataaggta ctgacgcaaa atactcttca ggtgtaatta
    11701 tatatctcga atttttacac ccaagaatat tagctgtctg ttgtgctaat tttgattcat
    11761 caaaacgctc ttcctcataa ccacatgaac ctgttttttc tatatctgac attgccaata
    11821 tataagatga atcaacacct cctgatagga ataactctgc agtttcattc tctgatttta
    11881 cctctggcat aattttctga agtgttgtat gaatttcatc cgtccattcc tcaatagact
    11941 tgctgtggtc aggatgaaat tcaggtttcc aataacgacc aatacatact gttttattct
    12001 gatagacaag gtaatgtcct ggcataagct tctttactcc acggaagaat gtattttcac
    12061 ctgctacata tgtgaaagac atataaatct gtaacatgtc ttcatttaac tctttcacaa
    12121 atcctggttg ctccataatc ttacgaatag atgaaccata gagaagcttc ccattttcag
    12181 tctcataata atagaaaggt tttaccccaa actggtctct taggcagaag agtttctttt
    12241 ccacagtatc ccataatgca aaagcaaaca ttccatgcat gtgatttgcc atatcatatc
    12301 cccatgtctt atatgcttcc actaaaattg ctcgttcacg ctcatctcta cataatgaag
    12361 aatcgattcc aagttcttca caaagaactt tccagtttcg aatatgccca ttgtattcta
    12421 gaatttttat catatatgtg tcttttcgtg ttaaatactt ttcttctaaa tttatcatat
    12481 gtgtatcctt tcgtattaaa acatttaact tctaaattta ttaaatgttt atatatattt
    12541 attcacttat aatcactgaa aaatcgactt ttttcaaagt aaattctttt tgaattagta
    12601 ctaatctgct ctttattaat ggcataaata tgattatcaa gtattatcag tgttataaca
    12661 ttgaattttt tattcttcat aaatattgcc ccctttatat taccaactaa atataatata
    12721 tcttatgtta cttacatatt ctttaagaaa ttctgaagat tttataaagt tttttgtatt
    12781 ataattaatt aaaaataaaa ttctagttat aaaacatatt ttccattaaa caaaaaaata
    12841 gttaaaataa attcataacc acataaactt attttaacta tttgtatatt ttaattataa
    12901 atattaactt aaatttttaa caggatacac tcataaaata aacataatga acaacaaaat
    12961 aatatgtctc agtttcttta tatactttac cttctttcaa ttttctacca tgtagacgta
    13021 taagataata ttagtaattc actctattgc ttaactcgtg cgatatgtac aaccaagaaa
    13081 gctatttcct catttgttaa cttttttcca tatttctttt ctaaaaaatc tctaactttt
    13141 aatgcaatta acatttcttt tggatttgac cttttcattt gattaaataa taaatcatca
    13201 ccactgctta acatactatc tgaaaaaaat ctttccacaa aaaattttat atgtgttata
    13261 aatctcatat aatgcatact ctttttatct aaggttctat tcaaagaaaa aacaaaaata
    13321 ttaattattt ctccaattac tttggcatac ttaatcgtat catatgaatt tccatctgaa
    13381 gccatagcat ttgcaaaatg gaaagcaatg ttagctgctt cttcctctgg taattgaatg
    13441 cccaatctct cctctaccat ataaattgca accattccaa ccttaaactc atttggataa
    13501 taatttttta tttcccaaaa tactcgattt gtaattttta ttcctttacg catcctttca
    13561 attgcaaagt taaaatggtc tgctaatatt aagtataagt ttttattaaa agatgagttc
    13621 aataactttt ctgcctctac tattatttcc tgtgttatct ttaaaatgtc gacaggaata
    13681 tcatccatta attctaaaaa ttgttttgat tttgtatttt caacagggac aaacatttga
    13741 ttatcattgg aaccacaaat atattctcct gtttttttac catatccaat tcctttccct
    13801 agcaaaataa attcattata ctttgcatca tgaactaaaa caacgctcga gttcaaaact
    13861 ttctttatta tgtagttcat tttatttgct caccttctat tggtcattta aaatctatac
    13921 tattattttc aattaccttt ttataccaat aaaaactatc tttcttatat cgattatatg
    13981 tcccctttcc atagtcatca gcatccacat aaatatatcc ataacgtttt gacatttgtt
    14041 tcgttgattc actgaccaaa tcaatacaac cccatgaagt atatccccaa agttcaacgc
    14101 catcttcgat aatcgcatct aacatacatt taaagtgttc ttttaaataa ttaattctat
    14161 attggtcatg aactttttta tcttcagtta aaatatcttt agctcccaat ccattttcaa
    14221 ctataaataa aggctttctg tatctatcat ataattcaat caaagatata cgtagaccta
    14281 ttgaatcaat ttgccaaccc cattcactca tttctaaata aggatttttt acagctaata
    14341 acgtattccc aggtgtaata tctaatccag ttgtatcagc tgcctcacaa gaagacatat
    14401 agtaagagaa tgaaataaaa tctactggat actttttcat aatttcatca tcatgttctt
    14461 cttttactat ttgaatgtta ttatttttaa aatacgacag taaatatact ggatactcac
    14521 caaaaacctg cgtatcacta tatgcataaa tacttctcat tttttgttga gttgccaaaa
    14581 catcttctgg tctacatgta tatggataat acgttaattt tgttaacata caccctactt
    14641 ttgaatttgg aataatctca tggcaaattt ttgttgcaat ggcactagct acaaattgat
    14701 gatgcatagc ttgaaaaata acttcctcaa agtttttatc cttaaaacgg tctcgaacta
    14761 aaccaccagt agtgtacgga tgacgaatca tagaatcaac ttcattaaat gtaagccaat
    14821 atttcacttt gtttttatat ctttcacaga tagtttctac atacctaaca aacatattga
    14881 taacttgtcg tgaataccat ccatcatagt ttaaaacgat atttaatggt ggttcataat
    14941 gtgacatagt aacaagtggt tcaattccat aacttatcaa ctcatcaaat acatcatcat
    15001 aaaactgtaa accttcttca tttggctctt tatcatctcc atttggaaaa atacgagacc
    15061 aagcaatact cattcgatat gtcttaaatc ccatttctgc cattagagca atatcttctt
    15121 tatagtgatg ataaaaatca ctaccatgac gttttggata atttacttca tcttcaggat
    15181 gtgctaatcc ttcttctata tcttttgttg tgatttcatt attagattta taatctttca
    15241 catctgcatc aaagtgtgcc tttgctacat ctgatacaga ccaccctttc ccacctaaat
    15301 tccaaccacc ttcaaattga ttagcagctg tagctcctcc ccaaagaaat ccttctggaa
    15361 attttctcat atttatattc ctccctttac atcaaacaag atacttcctt tttttacatt
    15421 gccataagac tggtttaaca ctttataagc ataatctgct ccattattgt ttacaatcat
    15481 aacagttgaa tctaaattag catgtgagat tttgtctaaa tcaaattgaa ttaataaatc
    15541 tccttctttt acattatcgc ctgtcttaac aaatgtttta aatccatctc cattcatact
    15601 tacagtattt attccaatat gaatcaaaat ctcagcccca ttttttgttc ttaaaccaat
    15661 tgcatgtttt gtttcaaata acatcacaac ttctgaatca caaggtgcgt ataaatttcc
    15721 attggttggt ataattgcaa ctccatctcc caaagtctta cttgcaaata gttcatcatt
    15781 aaccttactt aaatctatta cttttccttc tactggagtt actaagatat ttacttcttt
    15841 ttcttcttct agctcttctt caacaacatc tccaattgat gactctactg tatttgtatc
    15901 ttccttccaa ataatcagtg taataataaa cgagcctaat attgcaataa caaaaccaat
    15961 actcgcaaaa atcaaattgt ttccattgtt tgggtcaata aacatactca tactggcaag
    16021 ccctggtcca acaattacaa atccttttac tgcaaataat cctaaaaagg taccagaaat
    16081 tgcacttgag aatacaacac ctaacattgc tcttttatgt tgtaaggtaa taccatataa
    16141 agctggctct gtaattccca tcaaggcaga aattcctgca gataatgctg tttgttttaa
    16201 tgtttcatct tttgttcgca aggctactgc aaaacaagca ccactttcag aaatattgtg
    16261 ggctaatgaa gatgtcatat ataaagcttc ataacccaac tttccaattg tattaattga
    16321 ataaggtatt agtgctttgt gcattcctgt tgcaaccata aatggaagaa aagcagcaag
    16381 taatcctaca actaagaatc ccattttgtt atataagaat aaaataatgg ttgtaaaaat
    16441 tgtaccaacg ttatatccta aaggacctaa aatagttaga gtaattggaa cagtaattgc
    16501 caaagacatc ataggaacaa agaaagttcg aattggtttt ggtgaatact tgttaaaaat
    16561 tttctccatt gttgccaaga aagacacaca taagatagcc gggaaaacct gtgcattata
    16621 ggcaatgttt ggaattgtaa atccaaatag ttcaacacct tcacttaaag cagttgttgt
    16681 tgctggaagc aataagtatc ctacagcggc aatagctact aaacgattac atttcaatac
    16741 attggcagta gttacagcta ccataagagg taggaaatag aatgttgcat ctgaaatagc
    16801 aactaataat gtatatatag tagaatctga ttttaaaata ttaaacattg ctaataacat
    16861 caacagtgat tttaaaatac cagctcctgc aatagcaaaa atcagtggtt gaaaaatatt
    16921 aataataaat tctataaatc ttgttcctaa gtttattttt tcttcagttt ttacagtact
    16981 atcatttgta tctaaagggc aaacttttaa aatttcatca tacacatcaa caacactatt
    17041 tccaatgaca acttgaaatt gtgccccatt aactatactc ataacacctt gtactttttt
    17101 taaagcatct tgattgactt tacttctatc aatcaactta aagcgtagtc tagttgaaca
    17161 gtgttctaaa gctaaaatat tttttgaacc accaacttgt tcaataatgt caatggcagt
    17221 ttttttataa ttcatcttgt aatctccttc tccataattt tttatgaatg gccattcaag
    17281 atgcaaaaaa ggcagaacct atataaacac cttgaaaaat caaagcaata tattcgatcc
    17341 tgcctgcatt accagtaaca tgtcttctat ttatactatt ttgtattatt atattaacat
    17401 ttgcctaaaa gaattgtcaa tactttttta aattttctga tactaactaa tagcaccttt
    17461 ataattcatt ttttatgtca attaaagtaa cagtagccac ccaccaatta gtatcaaaat
    17521 tgaacctggt attttttctt ttatattttc tttgaatata aaatgagcaa gtattaaagt
    17581 tattatcata gatacttgag aaaatggctc tgctatacta agttctagat tatttaaagc
    17641 aattaataat gctaaataag aatatccatt aatagcacca cttaaaattg aaagcccctt
    17701 tttattagta aaaatctcac aaattatttt ttgtttcttt ttaaatgcta aaaagataaa
    17761 taaatttaaa cctacgaaaa aatacagtat agttgaatat aatataggag atacttgtac
    17821 aaaaaaagcc ttatccaata ctcttcctac tgactgcaaa aatatgtata aaaacattaa
    17881 tctacatggt aaattgttaa aaattacaga gacattcttt aaaaaagaac ttactttttt
    17941 taatataaac actccaccaa tcataattat gattcctgca acttttgcta aacttaaacc
    18001 ttcacctaaa aatataactg ataaaagcag taaaaacaat gaatttaaac tatttattgg
    18061 agtaacaagt gaaacttccc cagttgctaa agacgttaca tacgctactg aaccaattga
    18121 atagactaaa ctacttatat aacatggtat taaaaacgac caatcactaa ctctatccat
    18181 tatgctaaat ggaagtaaac atactccccc tataaagaaa aatagaaatg atgaagctat
    18241 atttttataa atatctcctt cttcatcacc taataatctt acaactactc tttcaaaagc
    18301 taatagcact actctaatta ataatgcaaa ataagataat atcatttttg acctcgctta
    18361 aaaattaatc cgacttattg ttatattaac tgtataagac taccatatag gtaagtcttt
    18421 ttgatttttt aatattttat gtttaaatac aggtacatta agtttttcac ctagtttacg
    18481 gcattgctct ttataggtac cttttaattt atcatgaaaa actatacaat caattcctcc
    18541 ttcaatgcta tttatacact tttctagttt ctcaaatcca gattcatcat catacattcc
    18601 acttacaatc cattgatttg gcaatacatg tccaattgat atgttatatt ctttattttt
    18661 tataatatct gagaaagatt tctcaaaatg tacccctcca acacataaca aagtcttcaa
    18721 aggttcatca caatcaaata ctctaattaa tgattttgcc aatacctgta ttgctatact
    18781 attattaaaa ctatcactac tacttcctat ctcaatatcc aacataggta cagaatattc
    18841 tgtaattaat ttagattctt caccatatgt tgttccagac cagtgagttg cttctgttaa
    18901 tgtagtaaat tcatcaagta gattttcatt ttttatatcc tcaatagcat tgattagatt
    18961 tttaaagtat cttggatttg aatttccaaa ttctcctgtt ggaacatctc caattgtatg
    19021 agctgttagg atatgttctg gagcattttc tccctcatgc caatttacaa ctcctgcaaa
    19081 atcataatct ctaaaatgtc tatttaaaat tggaagataa tatttataat tatgacttat
    19141 tacatctttt gtttttacaa aatgaaataa gttattgttt ttatccttaa actctaatac
    19201 aaaattatca tcaataacaa tatcagtttg atttaaatcc attatttctt cacaacattt
    19261 tatcacatta ctggctactg ggtctttctt taaatcactg caaaagaaaa aaactgcttt
    19321 tttattattc atccataaat cacactccta taacttaaat ataatatatc ttcataccta
    19381 aaatcatcta aatatcactc taattccatt aaattattta tatataatat actaccatac
    19441 tcattttagc tatacctttg cctgagtttc tatatatatg tgatacatca gctttaaatc
    19501 ttatagaatt tccactagat acaatgtaat cttctccatt tattttaatg gttacttcgc
    19561 cactaaaaac agttataaac tcttgtgttc ctggaagttc agcatttgaa tctaaatttc
    19621 caccttcttc tatttcaatc ataaatatct caaatcttct tttggagtca aatggaaata
    19681 ttggataact tatatacttt ccattatctt caataaaatg gtctatatca cttttttgaa
    19741 tcaattctat gtcactttcc tgcctctcca tcaatgatgt aaatgaaatt tttagcccat
    19801 tagttatttt cttcaaagta gatatagtag gatttacctc acacttttca atttgagata
    19861 gcatactctt acttacacca ctaagttgtg caatcttgtc taaactaaga tgtttttctt
    19921 tccttatctt ttttaaattt tcagcaatta tttcatttaa actttgcata aataccccct
    19981 aatacatact aatataagat aaattatttt gattattaaa taaacttagt aatttaatag
    20041 aaacttttta tccattataa tgtacattat gtttaatata cattactttt tttagaatgt
    20101 aaatagtttt ctgaaatttt gtgaaaatga ataaataaaa aactagtaac ctatattttt
    20161 acaaggttac tagcttttat tataataata tttcatttta actataaaac ttagatgcac
    20221 ctataagctg tctcttagaa acaatcttag ctaggtttgc taatgaagaa atttttatat
    20281 ctttaaaaga aactaaaaat acaaacttaa aatctgtaaa ttcttgattt atattgtata
    20341 tatcaaaata acatctcaaa ttatcaaata agtaattatc ctgtaagtct gcttctatct
    20401 catcagtagc atcttttaaa tacgctctta catcttcaaa taaaacactg aaataagact
    20461 catctatttc ttcacctaac tcttccaata tttcttctat atctaaatca tccatatcaa
    20521 aagaatttcc tgtcaactgg caatcaaata ccaatatatt ttcttgattt atagtatttg
    20581 atatatcttg tacatattcg aagtctggac gtaattctaa aatgtctttt atttcactgc
    20641 tctttacatg gatttttcct tttctatttt caatttttaa atctatcata tgctccccct
    20701 ttcaatctac aaatctttaa ataccttgtt ttataatctt ttcaatatct atccaattac
    20761 taactctaac tatattttga tgtttaacat ctttattata gttagtatcc agaagtatta
    20821 ctttaacacc atcatcagct agttgcactg agtttaaagg attatcttct ataaatatgt
    20881 cacaactcag ttctcttgcc ttttcaactt tataatcact tcctaataag taaacatcta
    20941 tatctgaaaa tccttgtctc gatagccaat ttttagttac atcagttaaa cactcatgtc
    21001 tagccgttac aaaagataaa ttatgtcctt tagataatgt gctaattacc tctgtagcac
    21061 cctctacaat ctttgcttca tcatatgagt gtatatactt attattaaac tccgcataca
    21121 catctcttat tcccgaacca tacaattctt cccaattatg tgttatacac tctgcttcag
    21181 ttatattttt attaaagata tcatttaggt atggaagaaa atgataaggg cttgttattg
    21241 ttccatctat atcaatacat atattcaatt tgttcatttt tacctccatg taacatttat
    21301 tgatttgatt aatcaaatat tatagcacag ctttaattat aaatcaaaac tctggatatg
    21361 actataccta tataatacca aaaagcttgc atatttaagc aagcttttcg taaattttac
    21421 atttttaact aatatataag tttttctaag gttggttcca ctccaaattg tgatttcagt
    21481 tctttatatt cttttaatgc tttgtcatta ttcgttttag tttttatcgc tcttatcatt
    21541 aaattttttg gtgtatcaag tggagatatg tattctacta cagaagtatc atatccattg
    21601 ccctccaata acaatgttct taaaccatca gttattaaat ctgcaaatct agctttaaat
    21661 acaccatgtt ttaatattgg ttccatagct tcatatctat attgccctaa taactcctta
    21721 tgacaacatg gtactactac tatagcttca gattttgctc taattcctag acctattgcc
    21781 atatctgtag ctgtatcaca agcatgtaga cttattacaa ggtcaacatc cctattaggt
    21841 gtataattag tcaaatcctc ttttataaat gacatatttt tataacccaa gtttttagcc
    21901 atattttttg atgcttctat aactacatct gaataatcta ttcctataaa ataacagttt
    21961 ttcttcaata cttcttttat ataaaaattt aagacaaagc ttaagtaaga ttttccacaa
    22021 gcacaatcca agatagttat acagtcttta tcctttattt cttttaaaat actatctatc
    22081 aattcaacaa aatggtctat ctgattgtat tttcttattt tatcattttt tatcttacca
    22141 tcttttgtaa gtactccaat ttcttttaaa agtgcatttg cttgtcctac ttttacataa
    22201 taatctctgt tttttatttg agatgccgta aattcatcta ttttaggtac ttcttcctta
    22261 ttatctaaat attttacatt taccttctta ttgtctcctt caattacaac aatagttcct
    22321 cgctcaacat atttaaatac taatccatca taattttctg cctctttaga aatattctta
    22381 agcaactcat taaacgtata taaatctgat tttcctttaa aattaaattt atatttatta
    22441 tcttcctcta ttgcaattcc tttaaactct tttcttccag atttaaaaat cacctctaac
    22501 tcaataaaaa aatccctatt atcaaggttt ctttgagtaa tccccataaa aaacatattt
    22561 agttttccta tactctgttt attcatcata atctcccttt taacttatat ctttaaatct
    22621 tattaattaa tattatttgt ttctgaatta tttatttctt cttttaaatc gttcaatcta
    22681 tttttaaaac tttctatctg cttatctgat aaatcaagat attctgttat cttccctata
    22741 tatttagggc ttttagtatt tattaatttc aaagcacttt ccatagattt ttctctaagc
    22801 tcatatgctt tctttacgtc atctctagca ttactcaatg tttctgtata cttctcctta
    22861 gaaagttctg gaatctgcat attttcatat tcttttacaa cttctttaca cttgtttctg
    22921 gcatttgtaa catctttttt tatactagat aatttagtta tattttttgc tatatcctta
    22981 gtgtaaggta tacattctct cattggaact aaagctgctc tttctatatt ataaatttct
    23041 ttcttaaaaa ttcctatact ttccatatct atttttttta tatcttcact atttttttct
    23101 ttacttgtat atttattatt ttcttttgta ttatcttctt gtttttctga tttattattc
    23161 actgaaacat tactatcaga attattcttt tcttttatat cattacttcc ttcttcatta
    23221 ctttctttat tttgtatatt tctatcttgt ttgttgttta aatttttatt atttgatatt
    23281 tcttcattct ttttattatc ctgttttaaa tcagtacttt ctttttcctt aaaatttgat
    23341 acatactttt catcatatgt ctcatcagaa atataattgt gataatacat agactctttt
    23401 attttattaa gtccactcat ataaatatta cttatcatta ttgttgtagc taataccaca
    23461 taaatactac tgtagataca agacctctta aaattcttgt aatttctgaa tatcactact
    23521 aaaggaataa gatataaaaa tataaaaact aatatccacc tcataagtcg tgtctccttt
    23581 ctcaaaaccg aaatccctca aatatttatt tttaatcatg tatcttatag tatttaccaa
    23641 tttatgtttt ttaatcgtga acttttatta aattaaattc tgtatagtca caagatgcta
    23701 atgtatattc tactccattt cgaatcccct tcaatcccat tttaaaagat tctttaccat
    23761 gcaagtgacc ataaatcacc ttttcaacat tatattcttc aaataacttt gtaaataaag
    23821 attcttccaa tttatcattc gtaggaggat aatgagtaat cacaattatt tttgagtgac
    23881 cacttttacg agcagattct aatgatagtc taagtctatg ttcttctctt ttataaactt
    23941 tttcatcagt ctcatcaaat ttaacatcat taggacatat ccagccccta cctccacaaa
    24001 ttgcataatc tttatattca taaaaatttg tctgaataaa acgcatgtct tcatatagct
    24061 tatttaaact tgtaacggtt gtccaccagt aatcatgatt tccctttata agtattttct
    24121 gacctggaag tttgcttatt atatctaaat cttttttagc ttcggataaa ttaattcccc
    24181 aagatgtatc tcctagtact aagaccatat cttcttcctt aacaacttct ttccaattat
    24241 ctataatttt cttttcatga ccatcccaat tacttccaaa gatattcatt ggcttattga
    24301 ctgatgttga aaaatgtaaa tcacctatag catacaaact cataatatca ttcctttctt
    24361 aattacacta aataggctgt ctctttttag aaattatttt ccattaagag gcagccccat
    24421 taatatttag caaattttct acaagtagtt tttactgaac tactttatca tctaccttag
    24481 tatccttata ttgctctaat aatatatcta tttcttgttt taattccaaa atttcagtta
    24541 tatttttatc tgtttcagtt ctatcaagtt ttctttggta ctgcatagct ttatgtgtat
    24601 cacccaagtc atataactct tgtattctat ttaaagtatc tctaatagct atagacttca
    24661 tttggtacat agcctgagct ttcattatgt cctctcttat ttcactcatt tcttggtcta
    24721 aaagaaaggc agcatcagat gctcttctaa gcctttcagc aagagtctca ggtatgtaat
    24781 ggtcatattt atattttaat gaatgttcta ttgtagccca gaaattcatt gcaagagttc
    24841 ttatttgtat ttcacatata atttcctttg aacccgcaat ggagtttata gggtatttta
    24901 ttataacatg atagcttcta taaccactat ctttgtaatt tgtaatataa tctttttctc
    24961 ctattatagt catatcattt cttactttta tcaaatcaac tatagcatat atatcctcaa
    25021 caaactggca cattattctt ataccagcaa tatcatcaat ttcagtttct atatcttttg
    25081 catttatcct ttttaactta gatattatgg aagatatttt ttttgttcta cctgtgacaa
    25141 actctatggg agaatattca ccttttgcta aatattcttt tcttatattt ttaaatttta
    25201 ttttcaattc ctctactgca tgttcataag gtgctagtat ctcattccat ttttcgtatt
    25261 ccatattgct caccctttat ttgtttttta tgaccttctt tattttatca taatttattt
    25321 tgtttttgta tagtaaagca attctccatt ttccaatata taccctattt cttctaaatc
    25381 aactaagtaa cttatgacag atactaacga agatttaaaa aaatgatact ctttataatt
    25441 attgcttaaa ttattgttat ttataatgtt ttttaataaa atttctaaag atttcggttc
    25501 tacaagcaac tctcttattt gatataaata tttttttaaa gcattcaaat gtttttctat
    25561 taatttataa gaatcttctt tcgatataac ttgcttgcca tgccctaaaa ctaagtactc
    25621 aaaatctata ttttttaatt tttctaatga atttatttgt tcttgtatat catatagaaa
    25681 caagaaatcg tattttgata gaatttcatc tcctatcaat aaatcgccaa caaaaatcac
    25741 attatccttc gtcaaaacag ctatactacc aggtgtatga ccctgaaatt caattatatc
    25801 aaacatttca ttgtttaatt caatttttcc agcttctata gatttatcta tttttatatc
    25861 atccaacatt ttatttttta agatattatc cataaataca ttgctctttc ccccaacaat
    25921 atactttgaa aacaattctg gattttctat atataattta tcgtattcag atgacattat
    25981 acacacatct ttataatatt ctttaaattg attacaagct ccatagtggt catcatgacc
    26041 atgagtattt ataatatatc taacctgtat attattttca tctaatactt tatttactct
    26101 cctaggtctt gaaccacata accctgggtc tatcataagt gctgtgttat cttcaaatac
    26161 atatattcct gtattcgtgc caccttttat aaaaaaggta tttcctttta cttttgtaag
    26221 attcaccttc ttcacctcat tttgacaata tttatgatac aatttattac ttactaatat
    26281 aaaactatac aagaacaatc ctttttattc tatctatagt tctttttctt attttgtatt
    26341 ctttagatat ccttattttc acattatatg ctaaatttct ataaagtaat gactctataa
    26401 aatattcaac tatacctcta tccctaaagt ctatatctat atttccactt gaataaatat
    26461 ttgagtctaa tatcataagc tcatcaaaaa agcttaatct cttagattta cttaaataat
    26521 ctttatttgg aataaatcta tcacaataat ctatcctgta atttactact tcatcattta
    26581 tttcaaatcc atattgcttt ttcattaact ctctatagtt attatttttc tcaataacat
    26641 tatctaaatt tttattataa tcctcttttt tccaatgtcc aaacatccta caatttaatg
    26701 gtctaacttc atatattaaa catctattat tagtatcttt aaatggacat gggttctttc
    26761 ttgtatactc ctcaaaataa taatctaata ttttacttat acagcttttt tttaaatcaa
    26821 ctctatcttc aagataacaa aatatattca agaactcaat taaatttata cctactgact
    26881 ccatacaaca atttccacat ccagaacaat ttcctactgg caaggactta tatacatcat
    26941 ttagtttgtc aaagagctta tttttaatgg aataatctat acaatttaaa atatctttat
    27001 tttttatact agtcattatt attactcctt gttataatta ttaagttaat acaaacaatt
    27061 attataacat attttttaaa tttaatcgca tatggaggta ttttatgaat agatatacaa
    27121 aaataattaa tatgatggga agttattaca caaaagactt tgaaaaagaa aaaaagaatg
    27181 taataaaagt tagagaagtt aaagaagata cagttagaaa atttttctta caaggtgact
    27241 gtgaagttct tgtagtattt gaagacactg ggaaagaaat acttattgat gacttttctc
    27301 ctgaagaaga tatcaaaaaa tatttaggag caaaatttat aaataaaaaa agatagttat
    27361 caaaaataat tctaaaa
//
LOCUS       DAYAOX010000030        27087 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.030, whole genome shotgun sequence.
ACCESSION   DAYAOX010000030 DAYAOX010000000
VERSION     DAYAOX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.030"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            119..916
                     /locus_tag="ACGI57_001032"
     CDS             119..916
                     /locus_tag="ACGI57_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HFG9716118.1"
                     /translation="MEKREKRIIISSLLSVSILMGLASIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            1026..1616
                     /locus_tag="ACGI57_001033"
     CDS             1026..1616
                     /locus_tag="ACGI57_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFG9716119.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            1682..3379
                     /locus_tag="ACGI57_001034"
     CDS             1682..3379
                     /locus_tag="ACGI57_001034"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFG9716120.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            3593..4432
                     /locus_tag="ACGI57_001035"
     CDS             3593..4432
                     /locus_tag="ACGI57_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFG9716121.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            4949..6805
                     /locus_tag="ACGI57_001036"
     CDS             4949..6805
                     /locus_tag="ACGI57_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9716122.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGTYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    6888..7128
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    7194..7429
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7514..8731
                     /locus_tag="ACGI57_001037"
     CDS             7514..8731
                     /locus_tag="ACGI57_001037"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFG9716123.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            8848..9330
                     /gene="cdeM"
                     /locus_tag="ACGI57_001038"
     CDS             8848..9330
                     /gene="cdeM"
                     /locus_tag="ACGI57_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HFG9716124.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            9594..10859
                     /gene="hisD"
                     /locus_tag="ACGI57_001039"
     CDS             9594..10859
                     /gene="hisD"
                     /locus_tag="ACGI57_001039"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFG9716125.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(11062..11814)
                     /locus_tag="ACGI57_001040"
     CDS             complement(11062..11814)
                     /locus_tag="ACGI57_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9716126.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(11932..12201)
                     /locus_tag="ACGI57_001041"
     CDS             complement(11932..12201)
                     /locus_tag="ACGI57_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HFG9716127.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            12676..13770
                     /locus_tag="ACGI57_001042"
     CDS             12676..13770
                     /locus_tag="ACGI57_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9716128.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            13772..14722
                     /locus_tag="ACGI57_001043"
     CDS             13772..14722
                     /locus_tag="ACGI57_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFG9716129.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            14715..15899
                     /locus_tag="ACGI57_001044"
     CDS             14715..15899
                     /locus_tag="ACGI57_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716130.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            15912..17408
                     /locus_tag="ACGI57_001045"
     CDS             15912..17408
                     /locus_tag="ACGI57_001045"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFG9716131.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            17431..18381
                     /locus_tag="ACGI57_001046"
     CDS             17431..18381
                     /locus_tag="ACGI57_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9716132.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            18402..19364
                     /locus_tag="ACGI57_001047"
     CDS             18402..19364
                     /locus_tag="ACGI57_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFG9716133.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            19736..20368
                     /locus_tag="ACGI57_001048"
     CDS             19736..20368
                     /locus_tag="ACGI57_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9716134.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            20794..20871
                     /gene="kdpF"
                     /locus_tag="ACGI57_001049"
     CDS             20794..20871
                     /gene="kdpF"
                     /locus_tag="ACGI57_001049"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFG9716135.1"
                     /translation="MWFLAVVIIMLIIYLVYALINPEKF"
     gene            20918..22594
                     /gene="kdpA"
                     /locus_tag="ACGI57_001050"
     CDS             20918..22594
                     /gene="kdpA"
                     /locus_tag="ACGI57_001050"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFG9716136.1"
                     /translation="MVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDNFI
                     YKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGLSF
                     NTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKKKT
                     LGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGPVA
                     ALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKNKK
                     QGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSSLF
                     TTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVFLC
                     GLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGFHG
                     LSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGVVMFFGRYVSIIVLLAISSLLASK
                     KAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            22619..24676
                     /gene="kdpB"
                     /locus_tag="ACGI57_001051"
     CDS             22619..24676
                     /gene="kdpB"
                     /locus_tag="ACGI57_001051"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFG9716137.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            24695..25321
                     /locus_tag="ACGI57_001052"
     CDS             24695..25321
                     /locus_tag="ACGI57_001052"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFG9716138.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            25558..26466
                     /gene="cysK"
                     /locus_tag="ACGI57_001053"
     CDS             25558..26466
                     /gene="cysK"
                     /locus_tag="ACGI57_001053"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFG9716139.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            26493..27083
                     /gene="epsC"
                     /locus_tag="ACGI57_001054"
     CDS             26493..27083
                     /gene="epsC"
                     /locus_tag="ACGI57_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFG9716140.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
BASE COUNT        10689 a         2735 c         5018 g         8645 t
ORIGIN      
        1 tttagaagtt acaaaaaagt tacataaatt tggaaagcta taatatacat atataatata
       61 aggttaaaac tgtaatgaat aatattaatt taaaatttga acatggaagg agattattat
      121 ggagaaaaga gaaaagagga taataatatc atctttatta agtgtatcaa ttttaatggg
      181 tttggcaagt atatattcta tattaaataa agaagacata agtttaaccg ttaaaggtca
      241 agagcaaaaa gtatcttcct ttaagaaaac agttgaggaa cttttggatg aacagggtgt
      301 aaagtataat tctgaagata agattaatcc aagtttagat acagagttaa aagatgatat
      361 gaaaataaaa gttgttaagg taactaaaag taaaaaagaa gagattgaaa aaattccatt
      421 tgatacaaag catgtaaatg atagtaattt gttaaaagga aaatctaaag tttatcaaga
      481 aggtcaagaa ggagaaaaaa aactagtcta taatttaact taccatgatg gcaagttagt
      541 caaaaaagtc ttatcaaaag aagtaatatc taaggagcca actacaaaaa ttataaaata
      601 tggaactaaa gaaaaagtac taatagcatc aagaggagca aatataagag gaggcaaaca
      661 tatgaaagtg gttgccactg catatgcagg agatacaata acatctactg gtacaactcc
      721 aagatggggt gttattgcag ttgacccacg tgtaatacca tatggaacaa aagtatatat
      781 acctaaactg ggtatgactt ttgtagcaga agattgtgga ggtgcaatta aaggtaatag
      841 aatagacatt tttatgaaca gtgaaggaaa agcttcaaat tggggaagga aaagtataga
      901 tatatatcta cattaaatta gtgaatagaa taactttact tgaaggacaa aaaagtataa
      961 aatatgaatt aactaaactg ttagattatt cttaaaacat aaaaatatat ctaagggggg
     1021 ataagatgaa aatagtctgc ttaggagaca gtttaacgta tggatttgga gtatcacgaa
     1081 gtaattcatg gacaaacatt gtaaataaag agactcgatt agaaatagta aataagggta
     1141 taaatggtga tacaacaagt ggtatgttag ctaggtttaa tgaagatgtt gtaaaaaact
     1201 ctcctgatat agtttttata atgggtggca ccaatgattt tattgctggt gcgggaaatg
     1261 aaattattaa ttctaatata atggctatgg tgcatcagtc atattcaaaa aatattatac
     1321 ctataatagg tataccatta aaaccagata ttccaaatgt tagggaagat tggagttgtt
     1381 ttactgattt taatatagta tctcaaaact tagaatcata tagttattgg ataaaaaaat
     1441 tttgtatgac atttaatact aatttcgtag atttttattc agaatataat aaaaatatgg
     1501 aactagaagg atataaaaaa ttatattttg atgggttaca tccaaccaaa gagggtcata
     1561 gaataatggc agacattttt attaattcaa ttaataagta tatagaagag ttatagatta
     1621 cattaaaatg cttgtttatg cgtcagttgc aataaataca tacagtagag aggggtttat
     1681 tatggattat actaaaactt atgaagagtg gataaagggt tcatattttg atgaagatac
     1741 aaagctagag ttggagaata taaaaaataa tgaaaaagaa atagaggata gattctataa
     1801 ggatttagaa tttgggactg cgggtcttag aggaataatt gaagcaggaa cgaatagaat
     1861 aaataagtac acagttagaa gagctacttt tggattggca aactatatat tagaaaacac
     1921 aacaaaagaa gaaacaagta gaggagtagt aatagctcat gataatagac acaagtctag
     1981 acaattctgt atagaatctg caaatacttt ggcagcatgt ggtataaaag catatatatt
     2041 tgatagtttg agaactactc cagaattatc ttttgctgtt agaagcttaa atgcaatcgc
     2101 tggtatagtc ataacagcaa gccataatcc tccagaatat aatggatata aggtatactg
     2161 ggaagatgga gcacaagtta tgccagagat tgcaaatgca ataacagaga aagtaaatag
     2221 tatacatgat tatagtacaa taccaacctt aacggaagct aataaaaatt tggtagtttt
     2281 gttggacgaa tcacaagata ctaaatttat agaagcagta aaaagccaaa ttataagaaa
     2341 agatttagtt aaaaatgtag gtaaaagctt taagatagtt tatacacctc tttgtggaac
     2401 agggaatgtt ccaataagaa gagcattgaa agaagttggt tttgaaaata taattgtagt
     2461 tccagaagaa gaaaatccag acccaaattt tgcagggctt gattatccaa atccagaaga
     2521 gaaaaaagct ttaaatagag gtatattact cgcaaaagaa aaaggagcag accttgtaat
     2581 agcaactgac ccagattgtg atagagtagg tgtagcagta aaaactacta caggtgagta
     2641 tgctttactt acaggaaatc aaattggtgg tatgctgaca cattatataa tagaaggatt
     2701 aaaagaaaat aataaattaa aagaaaatcc tacaataata aaaaccatag ttacatcaga
     2761 atttggagca gatatagcta aagcaaataa tgttgatgta ttaaatgttc ttacaggttt
     2821 taaatttatt ggtgaaaaaa taaaattatt tgaacaaaat aaaaatagaa gttatgtatt
     2881 tggatatgaa gaaagttatg gttacttagt tgggactcat gctagagata aagatggtgt
     2941 agtttcttca ttgttaatat ctgaaatggc agcattttac tattcaaaag gtatgagttt
     3001 atatgatggc ttaatagaac tttataaaaa gtatggattt ttcaaagaac agactatatc
     3061 tttgacttta aaaggtatag aaggtgtaga aaagataaaa gaaataatat cttattttag
     3121 agaaaatcaa attgattata taaatagtat taaagtagtt gataaaaaag attataaaaa
     3181 tggtatagat aatcttccta aatctgatgt tttaaaatac tttttggaag atgaatcttg
     3241 ggtagctata agaccatctg gaacagaacc aaaacttaaa ttttacatag ctgtaaaagg
     3301 agctagtgat attgaagcag ataaaaagat tcaaggtttg aaaaagtata tagatgacat
     3361 ggtcgaaaaa ttaaaataaa atattattat tttataagat aattttgtaa taatatttat
     3421 ttagctgaaa attataattt actttcattg agtagaatat ggaaatgtta gtagaattgt
     3481 tattttcagc ttttatttgt gttattttta cttaaataat tatattatag aatgaatctg
     3541 agtataatta atattgtata atgaaattaa tttgatagaa aaggggatat ttatgtttgg
     3601 tatagataag aaattgattg aatatataat tgcaaaatcc aattacagtg aattaagttc
     3661 aaagtcagca gaagtatctt tcttcttaat gttgtccata tttccttttt taatatttac
     3721 aataagttct atagcttata taccgattct tcatttaaat aagtatatag ctttgtttag
     3781 aaatatgatg ccagaaggtg cttttgctgt gttatcttca atcatagttt ctgcaataga
     3841 caatagaaac ctgaaatttt tagcagtcag ttttgtactt actatgtgga cattttcaag
     3901 agcagtaaaa gctcttatta aaggaatgaa tagagcctat aaagttaaag aaactaggtc
     3961 tttttttaaa attttatcta tatcattttt atttacaata atgcttttgg tccttatttt
     4021 tttatctatg atatttttag tttatgggga aaaaataggt tattttattt ttaatcttgt
     4081 aggattggat gagatattta taaaaatatg ggatattcta agatacactg taggtataat
     4141 aacaataata gtgatattta cacttttata taaatacact ccaaataaaa agttaactat
     4201 taaagaatct gcgccaggtg ctatttttgc tacttttgct tggtttttag tatcattttt
     4261 atattcttat tatacaaatt actatgctaa ctatgaagta atctatggaa gtatagctga
     4321 aataattgta cttatgacct ggatgtattt tagtagttgg tctatcgtta ttggctatga
     4381 ggtaaattca agattgtatt ttagaaagat aagacatgaa atgttaaagt aaagtattta
     4441 aaataaaaga gagtatttag tattataaat gtaattgaat taataaagaa atattgacat
     4501 ttaagttaaa aagtctagaa tttttctggg ctttttttat ttttagtata acttgacaaa
     4561 ttttatttaa tgggatagtt ttaatttata atcatcttta aaaagatgac tattgataag
     4621 taagagataa aattggtaag tatcaaaaaa tatactaaat aaaaattata gtcctaatga
     4681 gttaatttct gtattttcaa ataatgcagt atttagtcat gtgtttttat atgcgcctgt
     4741 atttctaatt accaatatat tagaataagc tactgtaatt agatttagaa aaaagcgaca
     4801 taaataatta taaacaaata ataaattaca taaaattaaa tttattcaca agtatatctt
     4861 ataaaatatg ttataattaa tagaaaattg tcatataatt aattaagtag tattattata
     4921 aataacattg aaaggtagtg gttagaatat ggatacccat aataaatatg taaattttat
     4981 aaaaaatata cctgtacctt ttttatattg tagaattgta aagagacaag aagatataga
     5041 atatcgagta gaatatataa gtaagggtat gggcaaagta ttacaattag aagaaggtat
     5101 ttgtgataaa aatatactag atgtattgcc tgtatttaag tctaaaaaat atttcaaaga
     5161 gttgttctca aatgaagtgg attgtataaa aaggtacatt ccgacactta aaaattggat
     5221 aaatataaaa aaacagataa taggggattc atacataatt ttgtattttg gtaaaattgt
     5281 atttgattat agacagataa tcgattcctt tgataaaaag gaaaaagtag cttatattaa
     5341 agacgaagaa ggcacatata tagattgcag tgaaaattta ataccaatat taaataacaa
     5401 cattaaaaca acgaaagata tctttggtaa aaatgatata gaagtatggg gagagaatac
     5461 aggaaaatta tttagagatg attatagaga aggagtatct agtaaaaaaa gatttttaca
     5521 aaatcttttt gaatatgaag aaacattttt tatggttgaa aaatattttt tgtatgatga
     5581 agacgaactt ttaggtacta tagggatagt agacaatata atatatagtg gttacagtaa
     5641 tagaaattat aactcaaaag atttaatgaa gatgatagaa cactcaattc cagagaatat
     5701 gttttataaa gatgtgtatg gaaattatat tggatttaat tcaggttttt tgaatttagc
     5761 ttgtatgaat aaagaagaat tattagggaa gaatagttat aaaatatcag aagaagaagc
     5821 tttaatagat aagatatttg aaagtgataa gggtgtagtt gaaaataaaa aagtagttac
     5881 atttgagtta aatataagta tgaatgatga aaacaagtgt attgagatta caaaaaggcc
     5941 gttttttgat agctatggaa gtgttattgg aataattgga acagcaaggg atataagtag
     6001 aagaaaaaga ttagaagagg aaatggataa aactaggatg gaattttttg caaatttatc
     6061 ccatgaactt agaacgccta taaacttaat atcatcttca ttacaagtga tagaaaaaaa
     6121 agaagcagat ttaatagaat caaatgatac tttaaaaaga aatttaggta taataaaaca
     6181 aaatggaaat agaatattaa gattagtaaa taatgttata gattttacaa aaatgcaatc
     6241 aggatattta gattttaaac cagaagaatc tgatataatt gcatttatag aggagatatg
     6301 tatgtctgta gctgattttg caagccaaaa taatatacaa cttacatttg atacagaaat
     6361 agaagaattt tcaatgttat ttgattcaga aaaattggaa agaataatat taaatttact
     6421 ttctaatgga attaagtaca ataagaaaga tggaaaaata aatatatttt tatatgtgaa
     6481 agataatgta tttaatatga aaatatctga tagtggaata ggaataccaa aagaaaaaat
     6541 agataaaata tttaataggt ttgaacagat tgacaatgaa ctttcttaca gagttaaagg
     6601 aagtggtatt ggactttcat tagtgaaatc tttggttgag ttgcatgagg gaagtatatc
     6661 tttaaaaagt caacttggta ttggaagtga atttatagtt tcattgcctg ttagaagtaa
     6721 aaataatatt gaaaaatata atcataaaag agaaatcagc aatgaattaa gtaaaaaatt
     6781 agaaatagag ttctcggatt tataagttta aatataaaaa ataaaatttt aaaaaagtat
     6841 tgacaattga tttagaaggg tttaaaataa attacaacat aataaataaa aaggcgatga
     6901 ataggaagag taaatctaat tcgattacca gagagaagag tccgttggct ggaagacttt
     6961 ttaaattaga gggattgaaa actacctagg agctactttc tgaagtcatc tgcattagta
     7021 gattttagta agaatagtcg ttagctgcgt taaagtgtaa agtgagtttt catgacatat
     7081 gaaaactaaa ttaggtggaa ccgcgggaat aatactctcg tccttatgtt ataaggatga
     7141 gagttttttg ttatttaaaa atataaacag taatcataat aattatagaa aatataaaag
     7201 caatgaatag gaagagtaaa tttaattcga ctatcagaga ggaaagttca caggctgaaa
     7261 gactttctaa gtttgagaaa ttgaaaacta cctaggagct gtggtctgaa gttactttaa
     7321 aaagttttag taagactaac cgttaactgc gttaaagtgt aaagtggatt ttatcaataa
     7381 atgataaaat caaattgggt ggaaccgcga gtatagactc gtccctttat atagggatga
     7441 gtctattttt attgcaataa aagattgaaa ataaaaaaaa atcggaaaat tataaattta
     7501 ggaggaaaat aaaatggaga ataaagtgaa aattggagta ctaggatatg gagttgtagg
     7561 aagtggactt atagatataa tagataataa taaagaaaaa agaaatatag aaatagttgg
     7621 aattttagtt aataatttgg aaaaacataa ggataaaaaa tattctaata taataactaa
     7681 taatatagat gatatattta ataaagatat agatatactt gttgaagtca tgggtggttt
     7741 agaaccatca ttatcttata taaaaaaagc attaaacaat aaaattcatg ttgttacagc
     7801 aaataaagat ttgttagctg aatgtggaga tgagcttgca aaattagcaa gtgaaaataa
     7861 agtaagtatt aaatttgaag cttcagttgc aggaggtata ccagtattaa aaccaataat
     7921 agaatcacta gaaggaaata atatagatag tataaatgca attttaaatg gaactactaa
     7981 ttttatatta tctaaaatgt atgatgagaa tctatcttat gatatggcat taagacaagc
     8041 acaagaatta ggttttgctg aagcaaatcc agagtcagat gtattagggt atgatgctgc
     8101 tagaaaacta tcaatattat caactctagc ttatgacaat agagtatatt ggaaagatgt
     8161 gtatttagaa ggaattacag atatagatga aaaagatatt gaatatgcaa aaaaattaaa
     8221 ttgtaaaata aaattgattg gtcaaagtaa gtatgaaaat gataaagtaa gtgcttttgt
     8281 tagacctgtt ttagtagaaa aagataatat ccttgctaga atagacaatg agttcaatgc
     8341 tgtcatagtg aatggagatt cagtaggaga agtttctttt gtaggtaaag gagctggaag
     8401 tttagcaaca ggaagtgcgg tttattcaga tgttatagat ataatagata atagagtttc
     8461 aagtatagat tcttttacta aagataaaat acaagttaat aaaatagtta gagaaaagtg
     8521 tggagcactt cttagattta aaaaatgtaa taaagatgaa attctaaata tagttgagaa
     8581 ttgtttagta aaattcgata ttttaaatga tgatgatgaa cttgctatta tggtttatgc
     8641 tgattctgaa tatgaaataa acaacagttt atgtctaata aaagataaag gatactgcga
     8701 aaaaatgaat aaaatgttga aaataagtta aaaaagaaaa aatataagtt tatatgtaac
     8761 atattttgac gattttaata ctatatagta tgagatgaaa caaaacatct cataaaaaaa
     8821 ttaaaattaa agggagagat atgttatatg gaaaataaaa aatgttattc agaagattgg
     8881 tatgaaagag gagaatctac agctaaatgg ttccaaaatg atagagaaga atatgaaaga
     8941 gaagcatatg atgaagatag agaaagaaga ggttcaaact gtggatgttc agattcagga
     9001 gaaaatagac ctagaaactg tgaaagattt agaagagaag ctgagataag agaaagagaa
     9061 gcaagagaag cattctgtga atcttcagag aaaaagaaag aggcattagc atatgaatgt
     9121 gaagctagaa aattatggga agaagcagaa aaatactggg atgaatattc aaaatacaac
     9181 tataaaggaa tcgaatattt agcagaagct gctagattat ttgatgaagg tatggaatgt
     9241 gaagctagaa gaaatggaaa taatggagga aacaataata attgttgcca taaatgccat
     9301 aaatgtaatt gtaactgctg tagaaaataa aaaaattaat tgacaaatca atagttaaaa
     9361 tatatcatat tatgtataat aaagagaaaa ttcagaaaaa aataatgatt agaagaaata
     9421 taagtatatt tattagacaa atagtgctca gttagtgagg tgagtacaat atgattaaac
     9481 tcccatcctc tagcttttga tagagggtgg gagtttttat ttatttatat atcttaaatg
     9541 aggataatat tgtaaaataa atgagtatga gattgtaaaa ttaggaggta aaaatgaaaa
     9601 gtattaaaac atcaagtatg aaagaaaagg tagatgaaaa gatattaaga gagcaagtaa
     9661 gttctataat agaagatata agaaataata aggatgttgc acttaaaaaa tataatgaga
     9721 agtttgatag aaatacaaga gatgaattta gaattactaa ggaagaaata aaggaagctt
     9781 ataagcatgt agatgacgaa tttataaata acttaaagat tgctgccaaa aatataagag
     9841 aatttgctaa agctcaaaaa ctaagttttg aaaacccttt tgaaaaagaa atttatccag
     9901 gtgtaatctt aggtcaaact aatattccaa tagagtcatg tcttgcatat gttccaggag
     9961 gtcagtatcc attgttttca acagctctta tgcttataat acctgcaaaa gtagcaggag
    10021 taaaaagaat agtggcatgt tcaccaacta tgaaaaatac tgaaaagata aatcctaaga
    10081 cattggtagc tatggatata gcaggtgctg atgaaatata tgcaactgga ggagttcaag
    10141 ctatagcagc atttacatat ggaacagaaa agattaatcc agttgacata attgtaggac
    10201 caggaaataa gtttgtaact gaagctaaaa gacaatgtta tggacaagta gggattgatt
    10261 ttgtcgctgg gccaagtgaa gttcttataa tagcggatga aacttcaaat ccagtttata
    10321 ttgcagctga tttattagca caatgtgagc atgatttaaa tgcaagagga atattattga
    10381 caaatagctt ggaagtagca caaaaagttg aaaaaaatat agaaagtatg ctaaaagatt
    10441 tgccaacaaa ggacatagca tatagttcat gggaaaataa tggagagata atattggttg
    10501 atgatatgga agaagccatt aaaataagta atttttatgc accagaacat ttagaagttg
    10561 cagtaaatga gtataatgat atttgtgata gattaactaa ctatggttct ttatttattg
    10621 gaaatctttc tgctgaggtg tttggtgact atgtttcagg taccaatcat acattaccaa
    10681 cacttaaagc atcaagatat actggtggtg tttgggttgg aacttttatc aaaacttgta
    10741 caaagcagat atttaatgaa aaagctatac aatcattagc acctgtagct gaaaagctgg
    10801 caaaagaaga gggattatat gcacatgcaa aggctgctga ggttaggttt aaaaagtagt
    10861 aaaaaatttt attttggcag tcgagactta gtattagact gagataagga aaacgctgag
    10921 aacctgaata aatactatat tttgtgggtc tcggcgcttc ttttatatct gtatataata
    10981 gaataatact atctgtgtgt ttaaatacta acttaatatt tcttttctaa ataatgttta
    11041 tgaaatagtt aatattttta gttaatactt gaaaaaaact tttgctaact gtgcaaattc
    11101 atctgcattt gtcaatatag cagggtgtcc tccctgacta aaaatgtaaa tacttgcttt
    11161 tgaaataaaa gttgccattt gcttgtactc ttcttcaaga ttatgacgac acatttcgtc
    11221 ttccttactt cctgtaaata aaattggcat ttctaattta cacaattctt tatgaaacaa
    11281 agggcgtttc tcgtttgcac attgcaatag agcttttgta tcaagattaa caacattctc
    11341 ccaatctttt ccatgacacc attcatagaa ttttcgtgct tgaatatctt gctttgattt
    11401 ttcccttcca gtgattagat tatctatgaa gttatcatta agagtacgtc catcaaagct
    11461 atctgctata attgcttcaa ctaaatcggg acgttctaat ccagtattaa cagctgccca
    11521 tgctcctcca cttgttccaa ttaagccaac tttaccacaa ttcaaatgtt caatcagtgc
    11581 tattgtctgt aaggcttcat catgccatat gtcaggagaa aattgagaaa ctctatccga
    11641 ctgtccatta cctaaaaaat caattaagat acatctaaaa ttttctgcat ataatggcac
    11701 aagtgcttta aacatattag aagaaccagt attaccatgc aggaaaatta tcggtttacc
    11761 ttgtccatat tctttataaa aacaagaatt accttgatac tgaaaatatg acatatattt
    11821 tcctcctttt tatttgttaa gttaattata acaatatagg tatgaatgaa aaacctcatg
    11881 ttctttctta gaataaaaat gtgccacgca taaaacgtga cacatttcta attataaatt
    11941 ttgctgttta agtattcttt gtatgctttt ctctgtcaaa aagtatttct ctgataactg
    12001 cttgatactc attccatttt ggaaatttat acagatttga gaattacgag aagctaagac
    12061 ttgtttggtg tttgtgtttt caccccaatg ctttttgtgc tcttgctttt tgggaatata
    12121 aatcacttta ccatctatat attcttgcac ttgctcaatc agttctttag gcagtaagtc
    12181 aatcgaattt tgatagctca ttttgcacct cctaaataat tattctttag gattagcaaa
    12241 ggctactgcc actcacaatt aaaaataaaa ttttttatta tgtacaatag cctttgctat
    12301 gcacaagttc caattcccat aaactaaata ctcctttctg gtttgaaaaa ttcatctgaa
    12361 cattgatttc gcttttaaga tagagttcaa tgacaaattt acatttttat tatatatatt
    12421 cagatagata attgcaactg tctttgtatg aggatatttt attatagtca atactataat
    12481 atgtatttat atcatcaaac taatatgaac agttgaaagg aatatgaaca ataaaactta
    12541 gtcaggaaga ataattatga agcagacaat agagaaattt gattttaaag ttttattgaa
    12601 aatttattga caaatttaac ttgtaaatat tatactaaac ttaataaata aagtttataa
    12661 agtaaggtgt tgtctatgaa aaatatagtt ggaaaaccgc aaacgataaa aaaagtaaat
    12721 gaagatttaa taaaaaacat tataaaaaat gaaggtccta taacaaaacc agaaatttca
    12781 agcattactg aattgagttt agctacagtt aataagattg tagagcaatt agctttgaaa
    12841 aatgaagtta aagtaagtgg actaagtgaa tctactggag gaagaagagc tcaattattt
    12901 gaaataaatg caaacttaga acacataata gctttatatt attatagaaa ttgttgtata
    12961 ggggtagttg ccaatttatt aggtgaaata atttatcaag aagaatttaa tataagaatg
    13021 gatacgtatg aaaacgttaa ccaagatact ttttttgtta tagatactct aataaaaaag
    13081 gaaaaaggaa acaatataag ggctattgga attggtgtcc caggagttgt aaaaaaaggt
    13141 gtagttaaaa atataccaac tataaaaagt tgggaaggta taaatttaga aaatttaata
    13201 aagagtaaat acaatactaa agtgtttata gaaaatgata caaatattac tactattggg
    13261 atttatcaaa aacagtataa agacaaatat aaaaatatgg ctcttatgta tttagaagat
    13321 ggtattggtt caggtatagt tataaatggt gaattgtatt taggttctac aaattttgct
    13381 ggagaattaa gttatctaaa tatagaattt ggtaagtata actctaacaa cttaaatctt
    13441 gaagattata ttatgagttt aagaaaaaga tatttagaaa gtaaagataa taaatttaag
    13501 aaagaaattt tatctataat atcaaacttt ataaggactc ttgtatgtgt cctaaatcca
    13561 caagctatga ttatacagtg tagtatatta accaagagtg acatggagtt tatagaaaaa
    13621 gatgtaagca ataatgttgg agaagaaaat tttcctaaat taataaaagt agactcattg
    13681 agagaaggaa gtataaatgg agtaattagt atgtgtttaa aagaaactga ctatcaatat
    13741 tccttatcta ataaaaaagg aggatattaa tgtgaaatat ttggtttcaa tagatggagg
    13801 gggaacaaaa actaaatttt gtgtaagtga cttagatgga aacatactta aagaacatac
    13861 cactggttca actaattata agtcagttgg aattaaaaaa acatatgaaa atattaataa
    13921 tggatttaaa aaaatattaa aagatttata tatcgattat gacgatattg agtacactgt
    13981 atttggtata tctggttgtg attctcctaa tgattataag attataatgg atgaaatatt
    14041 aaaaattggg attaacaaag aaaaaatata tctggcaaat gatgcagttt tagcttttta
    14101 tgcacaagct gattcccctg gcttagtaat tgtggctggg acaggttcaa ttatactggg
    14161 aattaaagaa gatggagaaa tatatagagt tggtggatgg gggtataatt ttagtgattt
    14221 gggttctgga tatgatatag ggagaaaatt attaaagaaa gtattgcttt attgtgatga
    14281 atgtcatgag tatagtgatt tatttagttg tgtcttggac ttctttggag caaatagttt
    14341 tgaacaactt gcatatatga ttactgatat aaatgataat gttgaaatag caaacttagc
    14401 ttcattagtg attgattgtg ccgaacaagg tgataaattg gctattgaaa tattaagaga
    14461 atcatcaact gaactatcta aattagctca agtaatattg agaaaaatat ctaatccaat
    14521 gaaaatggat aaaagcgaaa taaatatagt tttgtctggt ggtacattaa ataaaactgt
    14581 atatgctcaa atgttaatga ataatttaaa aacagaaaat acagataaaa atttaaaatt
    14641 tatatctcaa gaaaatcagc cagtatatgg ggggataaga cttgctatgg cattagctaa
    14701 taggagggtc aagcatggat agaaaaaagg taatagtaca aatttttact ggaggtttta
    14761 aggctaaaga aataaagttt aatgatataa aatgtaaact actagaatta ttggactgta
    14821 ttgatataga aaaggttatc atgggatgga gtgtagatga agaattatat gtagaaacta
    14881 aaaaactatt aaataaatat aatgttgaac tatatctttg gttgccatgt ttttctgaaa
    14941 taggattact tgaaaaaagt aatttactaa ttgattatat ggggaaggaa gtcaagggct
    15001 atgctttaca agaggatgaa aattttgagt tttattgccc aaataataag gaaaatataa
    15061 aaaatataaa gaaaatatat agtacttatt ttagtgatat taaatttgat ggaatttttt
    15121 tagataaaat aaggtatggt tctttttcga ataaactaaa tggtgttttt aattgttttt
    15181 gtaaagattg tttaaatata tataaaaaaa ataatattga taaagagtcg ttaatgagag
    15241 aaatgaataa tgtgtctaag ggagttatag ataatgaaga tactccattt ggtataacag
    15301 aatacattaa tggaaaatac aaatttaaaa ataaaatatg ggaagatttt tttgaaacaa
    15361 aaaatcaaaa tatatacaag gcattattag agataagtag ttattttaaa agtaagggat
    15421 tgaaaatagg tatagatgct ttttctcctt ttatttctta ctttacaggt caagatttag
    15481 aaaaattaca agaagtagca gattttataa aaccaatgat gtatagaact acaaaagctc
    15541 cagctggttt accatttgaa tttgaaaggt ttattgatga atccatacat aagaataaag
    15601 agattggaaa aagaaatttc ttagatattt taggaattaa ggagtatgaa ggagatgtat
    15661 atcctataga atttgtacta gaggaaatcg attttatgac aagaaatctt aataaagata
    15721 tatatgtagg agttgaaata aataaaaagg aaattatatc tcctgttaca cctaagtaca
    15781 taagtgaaaa tatttcaaat ataaataaga caacagtaaa aggatatgtt ttatcttggg
    15841 atttgttgtc agcaccaaaa gaaaatattg atgaaattat aaaatggttt cggaattagt
    15901 gaggtgataa agtgagtaag atattggaaa tacaacaaat atataaaagt tttggaaagg
    15961 tagatgtttt aaaagggata aattttgagt tagaaaaggg agaagtaaga gctcttttgg
    16021 gagctaatgg agcaggaaaa tctacactta ttaaaatttt aggaggagtt cattctcaaa
    16081 ctaaaggtaa agtaattttg actggtaatg aaataatgtt taaaaatcca catcattcta
    16141 aagaaaatgg tattagtata atttatcaag aattaagctt gataccaacg cttacagtac
    16201 ttgaaaatat ttttcttggt agagaaattt taaaaggaag tgtgttttta aacaagaaag
    16261 acatgcttga ggaatatatg aaaatttgtg aggattttga ttttaatata gacccacata
    16321 tagtagtttc taatttaagt gtagcaaacc aacaaatggt tgagataatg aaagctgttt
    16381 catgtaattc tgaaattatc ataatggatg aacctacaac atcacttact aataatgaaa
    16441 aagatgggct atttaaaata ataagtagac taaagtctaa aggtaaaaca atagtctata
    16501 tttctcacat attagaggaa gtttttaaag tatgtgatag tgcaagtata atgaaaaatg
    16561 gaattatgat tggaacttat gatgtagagg atttaactaa attaaagata actcaactta
    16621 tgacaggaaa tgataatcac aaacttattg agaagaagga ttatacattt gcaaaatatg
    16681 aagagccacc agtactagaa gtaaagaata taagctggtc taacaaaata aaaaatgttt
    16741 catttaaagt ttatagaggc gaagttgtag gtcttgcagg tcttgtaggt tcaagaagaa
    16801 ctgaaattat aaatttgata tttggtatag ataataaaga tagtggagat atttttatca
    16861 atggtaaaaa agtagacata acttctccta aggttgctat aaaaaacaaa ataggattga
    16921 ttcctgaaga tagaaaaaat ctaggattaa ttttagaaca agctatatac acaaactcat
    16981 catcagtaca aattgataaa ttcaagaaat ttttgtttat agatagaaat aaagaaattg
    17041 agttttctaa aaaaggtgtt aaggaattag gaataaaagt tagtgatgta aagcaagaag
    17101 taaaagagct tagtggtgga aatcaacaaa aagtagtagt ttctaagtgg ttagagaaag
    17161 atttagattt aataatatat gatgagccga caaagggaat agatattgct gcaaaagaag
    17221 atattttcaa tacaatcaaa gaattttcaa gtaaaggaat aggagtaata tttatatctt
    17281 ctgatttaga agaagtaata agagtaagtg atagaatttt agtaactaga gaaggttcta
    17341 ttgtagatga tttaaaaaat gagaatatac aggttcaaga tataatgaac agtatattta
    17401 atgtataaaa aatgaaaatg gaggactgag atgaagaaag cttttgagaa gttaaattta
    17461 agagattatg gagtattatt aggtttttta gctttatgta tagctataag tattgcaact
    17521 ccatcatttt taggtaaaca aaatatactt aatcttttac gacaatcttc tataattgga
    17581 ataatttcag caggtatgac ttttgttata atatctggaa actttgatat atcagttgga
    17641 gcagtagcag ctcttgcagg tgctataaca atgaaatttg cgactggtgg aactaatttg
    17701 tttgcatcca tgttcttagg tgtagcgatt tgtgcagtaa taggattaat caatggtata
    17761 gtagttgcaa agataaatgt accatcatta atagccacta tggctatggt cactattgta
    17821 agaggattgc tgttgatgct tacaggagga tatccaatta ctgaaaatat accaatactt
    17881 gattacattg gaaatggata tttgtttaat ataccaatac cagtagtaat tttctttata
    17941 gtggtgttag cttcgtttat tgtacttaat aaaactaagt ttggtagata tgtttattct
    18001 gtaggaggga atcaagaagc aagtaaactt aatggtataa atgtagattc tcacaaagtg
    18061 aaagtgttta ttattaatgc tgtacttgca ggaatagctg gaatcgtact aacaggaaga
    18121 cttgggactg caactgcgat tgcaggtgaa ggatatgata tggatgcaat tgcatctgta
    18181 gtaataggag gtacatcagt ggcaggaggt tctggttctg tacttaaaac tgttatagga
    18241 gtattactta tgagtgttat aaacaacagt tttaatcttc ttggaataga tgtatatttc
    18301 cagtacatat ttaaaggatt aataatttta gcagcagttg gatttgattc ttatagcaag
    18361 aaaaaacttg cttcaagata aaaaataagg gggatttttt tatgaaaaaa ttaatgaaac
    18421 atttagcatt gttgctatca cttgttatga tatttggttt agttggttgt tcaaatggtg
    18481 gtgacaaaga aaaaaatgga gaaaagaaaa tagcagtatt actaccaggt tcaacaggtt
    18541 actttgtagc aactaaacaa ggtatagatg caaaatctaa agaattaggt gtaagcgttg
    18601 aatatgcaga tgctcaatgg gatgctagta aacaattatc acaagctgaa gattttatgg
    18661 ctaaaggggt agacatgatt gcattatgtg gagttgattc tgctgtaagt gagagaatag
    18721 ttaaagctgc taatgattca gacgttccta tagtagcatt tacaaattct ataggaagta
    18781 acccaactgg agaatttaaa ggtttagtta catatatagg acaaaatgag gaagaaacag
    18841 gagcattaac tggaaaaatt gctaaaaact tattaggaga aactggtggg aaagctgtat
    18901 taatagaagg agtaccagga actactccac aagtattaag aaaaaaaggt cttgaaaaag
    18961 aattaaaaga tagtaatata gaaatagttt ataatcaaac ttcaaattgg gagaaagagc
    19021 aagctttaaa agtaacagaa gatttaattc aaaagaaaac tgattttaat gttataatat
    19081 gtcaagatga taattctgct acaggtgcag gtcaagcttt aaaagatgct ggtcttaagg
    19141 ataaagttaa agtcataggt ttgggtggaa gtaaagatgg attaaaagct ataactgatg
    19201 ggttaataga tggtacaact tatatgtctg ctgtagaaga aggtggatta gtcatagaaa
    19261 aggcatctaa attccttaaa ggtgaaaaaa tagaacctgt tacacaaatt aaacaagttg
    19321 aagttaacaa agataatatt tcagagttca agggtgaatg gtaaattgtc atagataata
    19381 aataagattt ttagagttac tttagaaact atataaagta actctttttt taatttaatt
    19441 tagtgataaa ttattgaata gtatttctaa acaaataaaa aattaatata aacaaagact
    19501 taattatact aaattataag taaaactaat ataaacttat ggaaaaaagg aaaacgtcta
    19561 aagtattgga attactatag tgtaggtgta gtattaaaaa aacataaaaa aacataaaaa
    19621 aattgttgaa aaaaatatag aaattttgta taattaaaga tttgaaaagt gcggtataca
    19681 aagccaattc ttttgtatat aatatatata aattcagaaa aaggaaggga tatgtatggc
    19741 caggcggtta tttttagaaa ggattggact tatattttta ggttcagcaa tattagcatt
    19801 tggagtttat aatttttatt atttaaacaa cataacagaa ggtggagtgt taggtatttt
    19861 actactgtta aaaaatttat tcaatataca accagcaata gcaaatgttg taatagatgg
    19921 tttactactt ttagtaggat ataaattttt cggtaaaaaa ttccttatat attcaatagt
    19981 agcttctata actttttcag tgttatatga cttatttgaa gctataggac ctctagtacc
    20041 acaatcacaa aatatgcttt tatcaactat tttggcaggt gtaactgttg gaactggtgt
    20101 aggtatagta gtaaaagcag gttgtgcatc aggaggagac gatgctttag cattagtaat
    20161 ttctaagact acatctttga acatagggca agtttattta gcaacagatg ttattgtatt
    20221 gttgttatca ttattttatc tatcagcgtt tgatatcttt tattctttga ttgctgttac
    20281 aattagtggc aaggtaattg attttattta ttatcatggt aaaagtttgg atatggatat
    20341 aagtaatgat atagttccag aatgttaata taaattaaat aactttagat tttattaggg
    20401 gtggcagatt tgccaccttt tttgtgtcta aatctttata gaaagtataa tcccatctag
    20461 gtttttgcaa atctcaaata tagaattatg ttggaatgac tgggaatacg attgctagat
    20521 gtgcatttta aaaacccaat tttatattta tgaaatacta atcattaatg gatgtttctt
    20581 aatcctatct ttatacctaa aatgataaga taaatgaagt agcaatacaa aactaaaatt
    20641 tgtaaaagag attaaattaa atggaggagc taaaatgttg gatatagtta tgatagcatt
    20701 gttattcata ggatttgttt ccatgaagtt acttgctgat tggtgtggga aacaaactga
    20761 aaaaaaataa tgtaatgtat ggagggatta cctatgtggt ttctggcagt tgtaattata
    20821 atgttaataa tatatttggt atatgcgctt attaatcctg aaaaatttta aacagtataa
    20881 ggctaataag taagttgaat tggggggaac aatttttatg gtgcagatag taatagtgtt
    20941 agcgattttt atgattttag taatacctat gggcaagtat ttatatcata tagctactaa
    21001 tcaaaaaaca tttggagata gactatttga taaagtggac aattttatat ataaggtatg
    21061 tagtatagat aagaaaaaag aaatgaactg gaaacagtat gcattagctt tattatttac
    21121 aaatgcagtt atggtattta taggatatat catacttaga actcaaagta tgcatatttt
    21181 taatccaagt ggaattaaaa gtatggaaca aggactatca tttaatacaa taataagttt
    21241 tatgacaaat acaaatcttc aacattattc aggtgaatca ggactttcat actttagtca
    21301 gatgacagtg ataatatata tgatgtttac atcagctgca acaggatatg cggctgctat
    21361 ggcatttgta agaggtctag tcggtaaaaa gaaaacattg ggaaattttt atgtagattt
    21421 aattaggata acaacaagag tacttttacc aggagcttta attattgggc ttatattagt
    21481 tacacaagga gtaccacaaa cttttgctgg gacagaaaca gtgactacta tagaaggaaa
    21541 gcttcaagat atagctagag gtcctgtggc agctcttgaa tcaataaaac atttaggaac
    21601 aaatggtggt ggattctttg gttcaaattc atcacaccca tttgaaaatc caacaataat
    21661 atcaaatata gtagaaatat tatctatgat gatattacca ggagcttgtg ttgttgcatt
    21721 tggtcacatg ataaagaata aaaaacaagg ttgggttgtg tttggcgcaa tgtcaatcat
    21781 atttttaata ggtctagtag tgtgctttaa agcagaaagt gcaggaaatc ctattttatc
    21841 acaattaggg cttaatcaaa gtatgggaag tatggaaggt aaggaagtta gatttggtat
    21901 agcacaatca tcattattca caacagtgac tacttccttt acgacaggta cagttaataa
    21961 tatgcatgat acactgactc ctttaggagg attagtacca cttttaaata tgatgctaaa
    22021 tgtagtattt ggtggtaaag gcgtaggtct gatgaatatg ttgatgtacg ctataatagc
    22081 agtattctta tgtggattaa tggtaggaag aacaccagag tttttaacta agaaaatcga
    22141 aggaaaagaa atgaagctta ttgctttact tattatatta catccacttt taatactaat
    22201 gttctctggt ctttcagtag caataccagc agggcttgaa ggaatttcaa atcctggatt
    22261 ccatggatta tcacaggtat tgtatgagtt tgcatcttca gcagccaata atggttcagg
    22321 atttgaaggt ctaggagata acactatgtt ttggaatata acaacaggtg tagttatgtt
    22381 ctttggaaga tatgtgtcta ttatagtact actagcaata tcaagtttgt tagcatctaa
    22441 aaaagctgta aatgaaagta taggaacact tagaacggat aattttacat ttacaatagt
    22501 tttagtactt gttgttttaa ttgttggtgc tttaacattt ttcccagcct tagcgcttgg
    22561 acctatttcg gagcatctag tgttgtggca ctaaaaaatt gggagggagt aaagttttat
    22621 ggaaactaaa aaaacaaagt ttattacaaa agatattttc aaaacttcca ttatagaagc
    22681 ttttaaaaaa ttaaatccta aatacatgat gaaaaatcca gttatgtttg ttgtagaaat
    22741 tggatttata gtaacattga tacttacaat agtacctagt ttgtttggtg atgaaggcaa
    22801 taacttaaga gcttataatg ggattgtaac aataatctta tttataacag tcctttttgc
    22861 aaattttgct gaagctgtag ctgaaggaag aggtaaagct caagcagata cacttaagaa
    22921 aactagaaaa gatacagtag caagggttat agatacgtta ggaaatgaaa aaatgataag
    22981 tgcaagtgag ctaaaacttg gtgatattgt tttagtaaat gctggagaag ttattccaaa
    23041 tgatggagaa gttatagagg gtatagcttc tgtggatgag tcagcaataa ctggagaatc
    23101 tgcacctgtc atgagagaat caggtggaga tttcgcatct gttacaggtg gtactacagt
    23161 tgtaagtgac tggttaaaga taagaataac aacaaaacca ggagaatcgt tcttagataa
    23221 aatgatagct cttgtagaag gtgcttcaag acaaaaaacg ccaaatgaga ttgctttaaa
    23281 tactttatta gtagggttaa cacttatatt tatggttgtt ttaatatctc tttatccaat
    23341 ggcaaactat gcaggtgtaa agattccaat ttctactatg atagctcttt tggtatgttt
    23401 aataccaact actataggtg gattgttgtc agctattggt attgcaggta tggatagagt
    23461 aactagattt aatgtaattg caatgtcagg aaaagcagta gaagcttgtg gagacgttga
    23521 tactatgatt ttggataaga caggtacgat tacttatggg aatagacttg catcagaatt
    23581 tatagcagtt ggaaatgcag attcaaaaga tttaataaat tattctgtta tgtgttcatt
    23641 aaaagatgat acaccagaag gaaaatctat agttgattta ggtatgaaat taggttcaac
    23701 aggtaagaca aaagaggcag aggaagctga gtttgtagaa ttttctgcac aaactagaat
    23761 gagtggtatt aacttagctg atggtagagc tataagaaaa ggtgcttatg atagcataat
    23821 aaaaagagta aaagaatctg gtggtacagt gccagaagat ttagaagaaa atgtaaatcg
    23881 agtagctaaa ctaggaggaa cacctttagt agtgtgtgta gataatgaga tatatggaat
    23941 tatctactta aaagatacag taaaatcagg gcttgtagaa agatttgcta gacttcgaga
    24001 gataggaata aagacaataa tgtgtacagg ggataatccc ttgacagcag ctacaattgc
    24061 aagagaagct ggagtagatg gatttattgc agaatgtaaa ccagaagata aaatagatgc
    24121 aataaaaaaa gaacaattag agggtaaaat agttgcaatg acaggtgatg gaacaaatga
    24181 tgctcctgca cttgctcagg cagatgtagg tcttgcaatg aatagtggta caacatctgc
    24241 aaaagaagca gcaaatatgg tagatttaga ttcagaccca actaagatat tggaagttgt
    24301 tgaaatagga aaacagcttt taataactcg tggggctttg acaacattta gtatagcaaa
    24361 tgatgttgca aaatattttg cgattatacc agctatattt actcttgcaa tacctcaaat
    24421 ggagatatta aatgtaatga aactttctac gtcatttagt gcgatattat cagctttaat
    24481 atttaatgct ataattatcc catgtttaat acctcttgca atgaagggag taaaatatag
    24541 acctatgaaa tcacaagcta tgttaatgag aaatatgctt atatttggtc ttggtggagt
    24601 tatagtacca tttttaggaa taaaattaat tgatttattg attacacctt tagtaatggt
    24661 tcttaattta ggttaataaa tggaggatat aaaaatgaaa acattaaagc cagcattact
    24721 tgtaagtatt gtattgttag ttgtttgtgg acttgtatat ccacttgttt tgacaggtgt
    24781 aagtcaagtt gcatttaaag ataaagccaa tggaagtatg atagaagtaa atggtgtaaa
    24841 agtaggttca gaacttattg gtcaaagctt tacagatgcg agattcttta agggaagagt
    24901 atcatcagta aactataata cttatacaaa agaagattta gtaccagata aagatggaaa
    24961 tacatcttat ggaggtgtat catcagggtc ttttaattat ggagctacaa atccagaact
    25021 tcatgataga gtacaaaaag atatagaaaa gtttttaaag gataatccaa ctgtcaaaag
    25081 agaggatata ccaacagatt tacttacagc atcaggttca ggacttgacc ctaatataag
    25141 tccagcctct gcaaaaattc agataccagc tatagcaaaa gcgtctggaa tatcagaaag
    25201 taaattacaa aaaattgtag atgataatac ttccaaaaaa ctttttggag ttttaggtga
    25261 agatagagtt aatgtattaa aagttaatgt agaggtcgca aaaatattag gtcttatata
    25321 gtattttgtg gaattttagc aacaaaatat tacgcaagat ttaatttaaa ggttaaattt
    25381 taaagagtga aaatcgataa tgattttcac ttttttattt gaatattact aatatttgga
    25441 ataaagtgat tgtaagtata aaatccaaaa aaaggttgca ttatcaaaat gataatacta
    25501 taatatattt atcgttatca atttgataat agatttggac atataggagg aaatagtatg
    25561 ttatataata acgcattaga gttgatagga aacacacctg tagtaaggct gaataattta
    25621 ggctgtacta atgggtgttc taacatatat gtaaaattag aaaaagtaaa tccagcagga
    25681 agtataaaag atagagctgt ttatgaaatg ttagagggct tagagcaaag aggagaatta
    25741 aaaaagggag atgtactagt tgaagcaact agtggaaata ctggtattgc attatctatg
    25801 ataggaaaat taaaaggata tgaagtagta atcgtaatgc ccgaaacaat gagcgttgag
    25861 agaagagatt taatgaaggc ttatggagca aatttggtgc ttacagatgg taaagctgga
    25921 atggcaggtt ccatagaaaa agcaaatgaa cttttaaaag aaaatccaaa ctataaaagc
    25981 ctaaaacaat ttgaaaataa agataatcca aatgcacatt ataaaacaac agcagttgaa
    26041 attatgaatg atgtcaaaga tttagatata ttcgtatgtg gagttggaac aggtggtact
    26101 ttaataggaa ctgcaagata tttaaaagaa caaaatccaa atataagagt aattgcactt
    26161 gaaccaaaaa aatctccagc tatatcagaa aacaagactg gacctcataa aatacaagga
    26221 ataggtgcta actttgttcc tgaaaattat gattctagtg tagttgatga agtaatatta
    26281 gtagatgatg aagatgcatt tgatacagtt aaattacttg cagcaaaaga gggaatttta
    26341 gtagggatat cttctggagc aaatatattt ggtgctatag agatggcaaa aaagtatcca
    26401 gataaaaaga tagttacagt agctccagat ggagttgata agtatatgtc tatgggaata
    26461 ttttaataat tttatttgag ggagtgaata ctttgtttaa gaaaataaat aaagatattg
    26521 aatatataat gaagaatgat ccagcagcta ggtctaagat agaagttttt ttactttatc
    26581 catcagttca tgcaatgata atgcatagga tggctcatgc tctttataag aagaagaagc
    26641 tttttacagc aagattaata tcccagatat ccagatttat gactggcata gaaatacatc
    26701 ctggtgctaa aatgggggaa ggtatattga tagaccatgg aatgggtgtt gtaattggag
    26761 agacagctga agtagggaat agggtgacaa tatatcaagg agctacttta ggagctacag
    26821 gaaaagatac tggaaagaga catccaactg ttggtgatga tgtattaatt ggtgctggaa
    26881 caaaaatact aggaccttta aatataggtt caaattcaaa aattggtgca aattctgttg
    26941 tcgttaagga tgtacctaat ggtgcaacag ttgttggtat accagcaaaa atagtaaaaa
    27001 taagaaacct agaacctgta aaaaagaata aaaaagaagt gtcctatgag tatgatgaat
    27061 tagataatgt atattatata taggatt
//
LOCUS       DAYAOX010000031        26590 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.031, whole genome shotgun sequence.
ACCESSION   DAYAOX010000031 DAYAOX010000000
VERSION     DAYAOX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.031"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(40..633)
                     /locus_tag="ACGI57_001055"
     CDS             complement(40..633)
                     /locus_tag="ACGI57_001055"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFG9716141.1"
                     /translation="MVGAVAGLIVLLYRILLGQAGTWLSQILKFVEGSTIKIIAWFAV
                     LAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKIKQVWWRVLPTKFIGGFLSLMGGLA
                     LGREGPSIQLGAMAGKGISRALDRGKTEEKFLLTCGASAGLSAAFHAPLAGVMFSLEE
                     VHKNFSVSVLISVMTASLTADYISANFLGMESVFQFD"
     gene            complement(865..1401)
                     /locus_tag="ACGI57_001056"
     CDS             complement(865..1401)
                     /locus_tag="ACGI57_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HFG9716142.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1474..1887)
                     /locus_tag="ACGI57_001057"
     CDS             complement(1474..1887)
                     /locus_tag="ACGI57_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFG9716143.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2353..2907
                     /locus_tag="ACGI57_001058"
     CDS             2353..2907
                     /locus_tag="ACGI57_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFG9716144.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISSMNGTISDMQGSISSMDGTINSMQDSITDMQVEIKEVNSRLDN
                     MEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVKDTSDIK
                     NDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3515..3718)
                     /locus_tag="ACGI57_001059"
     CDS             complement(3515..3718)
                     /locus_tag="ACGI57_001059"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9716145.1"
                     /translation="MCLDAVIYMLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDI
                     VFSCIVFHHCTNVQNIFCIFWKW"
     gene            complement(3920..4453)
                     /locus_tag="ACGI57_001060"
     CDS             complement(3920..4453)
                     /locus_tag="ACGI57_001060"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFG9716146.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            4770..4901
                     /locus_tag="ACGI57_001061"
     CDS             4770..4901
                     /locus_tag="ACGI57_001061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716147.1"
                     /translation="MGKVRIGCKKEKYFYVRNPVKSLVDRITENEQFAIYHFTHKYN"
     gene            5220..5408
                     /locus_tag="ACGI57_001062"
     CDS             5220..5408
                     /locus_tag="ACGI57_001062"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9716148.1"
                     /translation="MPYKYFTEFCLLKATQFLKGTNELISDIAIKVVFHQIIYFSKCF
                     KEKTGYSPKVYRNIKKEI"
     gene            complement(6201..6773)
                     /locus_tag="ACGI57_001063"
     CDS             complement(6201..6773)
                     /locus_tag="ACGI57_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFG9716149.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(6835..7098)
                     /locus_tag="ACGI57_001064"
     CDS             complement(6835..7098)
                     /locus_tag="ACGI57_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HFG9716150.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(7098..7319)
                     /locus_tag="ACGI57_001065"
     CDS             complement(7098..7319)
                     /locus_tag="ACGI57_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HFG9716151.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            complement(7835..8371)
                     /locus_tag="ACGI57_001066"
     CDS             complement(7835..8371)
                     /locus_tag="ACGI57_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFG9716152.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(8389..9702)
                     /locus_tag="ACGI57_001067"
     CDS             complement(8389..9702)
                     /locus_tag="ACGI57_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFG9716153.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            10057..10497
                     /locus_tag="ACGI57_001068"
     CDS             10057..10497
                     /locus_tag="ACGI57_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HFG9716154.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(10681..10869)
                     /locus_tag="ACGI57_001069"
     CDS             complement(10681..10869)
                     /locus_tag="ACGI57_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9716155.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
     gene            11891..12286
                     /locus_tag="ACGI57_001070"
     CDS             11891..12286
                     /locus_tag="ACGI57_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HFG9716156.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            12329..14428
                     /locus_tag="ACGI57_001071"
     CDS             12329..14428
                     /locus_tag="ACGI57_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFG9716157.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNTSTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(14653..19863)
                     /locus_tag="ACGI57_001072"
     CDS             complement(14653..19863)
                     /locus_tag="ACGI57_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFG9716158.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSMDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(19878..22373)
                     /locus_tag="ACGI57_001073"
     CDS             complement(19878..22373)
                     /locus_tag="ACGI57_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9716159.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(23034..23321)
                     /locus_tag="ACGI57_001074"
     CDS             complement(23034..23321)
                     /locus_tag="ACGI57_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716160.1"
                     /translation="MRLNITKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(23341..23616)
                     /locus_tag="ACGI57_001075"
     CDS             complement(23341..23616)
                     /locus_tag="ACGI57_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716161.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(24271..24450)
                     /locus_tag="ACGI57_001076"
     CDS             complement(24271..24450)
                     /locus_tag="ACGI57_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6440 family protein"
                     /protein_id="HFG9716162.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(24735..26210)
                     /locus_tag="ACGI57_001077"
     CDS             complement(24735..26210)
                     /locus_tag="ACGI57_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9716163.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
BASE COUNT         8982 a         4595 c         2960 g        10053 t
ORIGIN      
        1 taactcatga ctaaagtcac aagtgttcgt agctattttt taatcaaact ggaatacaga
       61 ctccattcct aggaaattgg ctgaaatata gtctgctgtc aaagatgctg tcatcacaga
      121 aattaaaaca gatactgaaa agtttttatg gacttcttca agtgaaaaca taacacccgc
      181 tagtggtgca tgaaaggctg ctgataatcc agcacttgcc ccacaggtta acaggaattt
      241 ttcctctgtc ttccctcggt caagagctct agaaatacct ttacctgcca tagcaccaag
      301 ctgaatggaa ggaccttccc ttcccaaagc aagaccaccc atgagtgata agaatcctcc
      361 aataaatttt gttggcaaca ctcgccacca tacttgtttt attttccctg tcatttcccc
      421 ctcaagctga gggataccac ttccagaaat cattggctcc cagtccacca actttgatac
      481 aatccacgca agtactgcaa gtactgcaaa ccaagctatt atttttattg tagaaccttc
      541 tacaaatttt aaaatttggc taagccaagt accagcctgc cctaaaagta tcctgtataa
      601 aagcacaatc aaaccagcca cagccccaac cataagtcct tcaccaatca aaattacctg
      661 aaagtgctct gcccgtttga taatatgaga tgtatccttt ttcatactat tcctccttca
      721 tccaataatt ctacacaatc tgaatctcca atgactaaag tcatgagtgt gtacagatat
      781 tttttaatca acaaaaatag atttttactt actaaatata agaaaataat tgctcaattt
      841 ctcaataaat gtgaatttat tcttttaggt atttattctc cacagtcgtg cttcttccag
      901 caaaatattt attaattctt gctttctctg tgtatagatt ttacgattgt cctcaaactg
      961 taatgctaat ttttgtttat gactatcata gagttttgct ttgtctgtaa aagtatttag
     1021 ataatctcga aaattgatat aattattcca tgcaacacta ttccattcaa caacatgaat
     1081 atggtgagta cgagtatttt tctcaaaatc tcccatagca aaaagtaatt gttttggtac
     1141 atcctgacct cgaaaaacga ttccacattg ctctaacctt tcgaggtata atttagcttc
     1201 ctctaaagaa gcaacaccta caacaatatc aataataggc tttgcgctaa tactgggaat
     1261 agaagtgctt ccaatgtgtt gaatatcaat agcagtctta tcccatatac tttttaataa
     1321 tataatgaca tcttttgcac ttttatccca tatcatctga tgttccaata atttaaccat
     1381 accacatttt aaacctatca ctatatacca cctcaatata atccaccttt atatatatta
     1441 caattttttc tatcttgata aatagtgatt tagctatttc actttttctt gacactcgct
     1501 acattcccta tcatgaatag atataattcc tccacattta gaacaagtat atctctcctt
     1561 ctgcttttgc ataaacttct ctaaaccgta ctgctgaaca aatttactat tttccataag
     1621 gcttgcttga tatctcttgt tataactttt ttcaagattt tttatgagtt tacaaggaaa
     1681 attgaaacat tcaaaacaat aaaacaaccc tttttgacag acacaatcct ttattttgca
     1741 cttacggcag tgttctggct ttcctgtatc gccgttcaaa caaccagcac atggcttttt
     1801 gtgataacaa tgcttatagc aaaccataca attcattcca caaggagcaa acataatcgt
     1861 atcaatactc tcttttggca ttttcataac attttactcc tttcaaaaat ccaatttgtc
     1921 gaattgttat atattcatat tgtaacataa aaaatagcta aaaaattcat gcactttcat
     1981 tgccaataaa ttttcaagga aatatgaaag tttaaaacta agaataaaaa gagataaaaa
     2041 ggaagctact aatcaaaata ttcaaacaca agtaatcaat ataaataact taaggtatat
     2101 ttaaaagtat atgatattac cagcagtttt aaatacaaat ataaccacac atgcctcaaa
     2161 aacacttatt acagtatctt agaaacttag acaatacata agattcatta ataaaaaagt
     2221 tctcaatata gatatattta accctgtttt gaatagtaga taatcatatg attaaaaatt
     2281 aaatatgaca tagcgtttgt ggaatataac acctaaatta tgttataata ataaagcagg
     2341 agtgtgatag tgatggatga aaaaatatta gagttattac aggaaatgca aggtaatatt
     2401 agtaatatgc aaggtagtat cagtggtatc gaaggcacta ttagtgatat gcaaagcaat
     2461 attaatggta tcgaagacac tattagtggc atgcaaggca ctattagcga tatgcaaggc
     2521 agtatcagta gtatgaatgg cactattagc gatatgcaag gcagtatcag tagtatggat
     2581 ggcactatta atagtatgca agacagtatt actgatatgc aagttgaaat taaagaagtc
     2641 aatagtaggt tagacaatat ggaacctgat attaaagact taaaaactgg acaagttgaa
     2701 actagtagta gacttgatac tattcagagt gatgttacag aaattaagtc agatgtaaaa
     2761 attaacagaa atacattaga tacagtgatt aaacatacct cttcattagt aaaagataca
     2821 tcagatatta aaaatgattt aaataatata gagatagtca catctaagaa ttcgaatgat
     2881 attgctaaat taaaatcagt taaataatat actatagtaa aaaatacctg aaaatttcac
     2941 atacttcttt aaagttatac caaattttat acagaaatac tgattatccg acaacacact
     3001 ttacttataa ataatttatt aaaaaaatac tcattttatc ctaaaattaa cacgactaaa
     3061 tgagtcttga tataataata aaaaataagg gagattgaac actaaaaagt tgtgtatctc
     3121 ccttatattc tataatatag ttatataatt tttcagccta aaataaaagt ccatctttga
     3181 ttaaaacaca tccccttatc acttataaaa ggataaaatc ttgttttcat ttttccattt
     3241 atatcttctt gcttaagagt aataattttt tcttttgata ctttaagtga ttggttaaat
     3301 ttaaccaaac gactaagttt tgttcaacat ctatttttca tattttccaa tacttaatct
     3361 attttttata aataattctt ttatttctaa ctcacttaca ttttttacat aaaacgaagc
     3421 atataaattt caaccttatc ttgtatggtt cttaatactt aatgagtagc ttaagtatca
     3481 atcattataa atttttccct tacatttaac actcttacca tttccaaaat atacaaaata
     3541 tattttgaac atttgtacaa tgatgaaata caatacatga aaatacaata tcataactgt
     3601 tgtctaaaaa tagaagttct tcaacataac ctttcacaaa aaacatatta tctaagtatt
     3661 ttttgtctgc tccttgtttt cctgtttgta gcatataaat tactgcatct aaacatataa
     3721 ctactcacac gaaaaaagaa aataaacttc caaaatacaa gctctaaaaa ctacctctaa
     3781 agttgtataa tgtttactag gaataaggtt gtataacata tagtttaaat aatcattttt
     3841 taaaattgca agttattgct ttcaaaatta gatgttttta tatcaaataa atgattaaga
     3901 atttctaaat ttaatggctt tataatgcac tccctttctt tgggataaaa taatgttgca
     3961 cagatacttt ttctaaagat aacaatttta tttttctatc aattccacca gcatacccag
     4021 tcaaactccc atttgttcct acaacacgat gacatggaat aataatagag attccattat
     4081 gccctactgc accaccgatt gcttgtgctg acattttaga tattcctttt tgttgagcaa
     4141 ctttatatgc aatttctcca taagtgattg ttttaccata tggtatttgt tgtaaaattt
     4201 tccacactgt agtctgaaaa ggagtaccta ccatatggag tgatggtgta aaattgggtt
     4261 cttttcccga aaaataaata tctaaccatt tctttgtctg ctcgaaaatt ggcaattctt
     4321 tttcttgatg ttcttggtca agattataag cataatactt ttgattctca aaccaaagac
     4381 cagttaaccc aatattatct gatgctaaaa gaatatttcc aattggacta tggtaatagc
     4441 taatatattg catacaattc ctccttatat tctgaaaact tcactcaaat cattagacac
     4501 ttcttttcta tctaccatta tgtaaaaaca tactaatata atttccttct aactacactt
     4561 tcgttataat atctatcctc atatcatatt agttttatat ttacctgcat ctgttcatcc
     4621 ttttttccct ttccctttga tgaatataaa attcatacat ttaagcttcc tactgtatta
     4681 acttttatag aagtgtatat tataatttta tcactaaata ggccaaaaaa atatcgatat
     4741 agcatcaagt aatatcataa tactttcaaa tgggaaaagt aagaataggc tgtaaaaaag
     4801 aaaagtattt ttatgttaga aatccagtaa aatcattagt agatagaata acagaaaacg
     4861 aacaatttgc tatttatcat tttacacata aatataattg acgcaataaa gtgttttcta
     4921 ttctacaacg attaataagg ttagaaaaaa gtaaaaatga actgtatatg tatgaagcat
     4981 tagtttctgt ttctttcctt tggcttatta tgataaaaaa tattgttttg ctaccaataa
     5041 aacacaaaaa tataattaat atccatatgc aaaaaatttt acgttatatt gaaaaatact
     5101 attgttttac attatattga agggtactat tgtgaaaact taattttgga gtactatcaa
     5161 gcagtggtaa tatcaataaa tcagagtgct ccagatgatt tgaactaagt ttaaatacta
     5221 taccttataa atattttact gagttttgtt tattaaaagc aactcaattt ctaaaaggca
     5281 caaatgagct aataagtgac attgctataa aagttgtttt tcatcaaata atctatttta
     5341 gtaaatgttt taaagaaaaa acgggatatt ctccaaaagt gtacagaaat ataaaaaaag
     5401 agatatgatt ttttatttct tttgattatc caaagagtaa ataaaaaata ttgtaaaaaa
     5461 cttactaaca acggcaatgc tttgaactat ggtaatactt tgaacttttg attggacttt
     5521 ttttgctaaa ataatatatt taaatagcaa agtaatctgt ttaatcaata aaaaataaga
     5581 gataaaagaa atcaactaaa acttttacct cttattttga tagtttatca gagctaatat
     5641 ttacactctt tttatttttg atacttcatt aaaaatgatg cttactctcc attcactttt
     5701 aattcttcat taaaaatgat gcttattctc cattcacttt taattcttca ttaaaaatga
     5761 tgcttactct ccattcgctt ttaattcttc attaaaaatg atgcttattc tccattcact
     5821 tttaattctt cattaaaaat gatgcttact ctccattcgc ttttaattct tcattagaac
     5881 tatgcttact ctccattcac ttttaattct tcattaaaaa tgatgcttac tctctgttca
     5941 cttttaattc ttcattaaaa atgatgctta ttctccattc acttttaatt cttcattaaa
     6001 aatgatgctt attctccatt cacttttgat tcttcattaa aaatgatgct tattctccat
     6061 tcacttttaa ttcttcatta aaaatgatgc ttattctcca ttcacttttg attcttcatt
     6121 aaaaatgatg cttattctct gttcactttt aattctttat taaaactgat gcttattctc
     6181 cattcacttt caattcttca ttaaaactga tgcttgcact ctgtaaactc ataaacatcc
     6241 attaatgctt caccaaatct ttgatagtga actacttccc tctcacccaa aaatctcaat
     6301 atatctttca aatcagggtc atcagttaaa tttattaatt gatagtacgt agccaatgct
     6361 ttttgttcag cagccatatc ctcatgtaaa tcagttaccg aatcgccaaa gacattaata
     6421 taacttgtag taaagtttac tccattagaa tctactggct gaagaccatg tccaaaatta
     6481 gcataattac taccaagtcc agcagcctta agctcttctg gagtacaatt gcctattaat
     6541 tgatataaca ctgaagaaat tatctcaaca tgagccattt cctctgtacc tatatcagtt
     6601 aaaagtgcac gagattttcc agttggcata gtatatcttt gttgaagata tctagttgca
     6661 gctgccaact caccattagg tcctcccaac tgagtcatga tatattttgc cattctaggg
     6721 tctgcttgtc ttatgttaac tggatgttcc agtgtttttt gataaatcca catattatct
     6781 tactcctcct taatatatat ttaataataa atctaatttt aaagtttaat aattttattt
     6841 ataaaatgat ttgtcccatg gccaaggttc agatatccat gaccaaggat attcacttga
     6901 ttcatatcca aaattagtta atgctccata tttagattca taatcacaca cagcttgtgt
     6961 aaatttatcg cagtatctgt tgtatgaatc aagagctttc ttatcccttg gattgttatc
     7021 taaatataaa tttaattcta tacaggcaaa ttgatattct gaaattctct ctaaaagttc
     7081 tcttctattt aatgacatta gtacatgtta cccccttttt tagctctaac tctattttta
     7141 ggatttgcat atagccaatc attatagtta tttgactcta ttaaatctaa ttcaggaaat
     7201 aaagtacctc tttttaatgc tgtttttgga ttgtatgtgt catcataaat ttgacgattg
     7261 atgtatggtc tagcaaattc acaacaatag tcttgtttat cctcttttaa atatcccatt
     7321 aattatcctc cttaattttt agaattttaa aatagcatta tttttaaaaa ttgtatactg
     7381 gttctatatt ataactggta gttttaatag gtctttaata agattaaatg attaaataag
     7441 tatctttttt tcttatttta cctctaaaat ttttctaatt aatctttcat aaatatctat
     7501 tttaaataaa taatatagag tttatacaat tttttatagc ttaattacta tttactatat
     7561 attattcatt tttaaataaa atgtgattat tttttattaa aaacgatata aatatacaat
     7621 ttaatactat ttttttataa ataaacttgt attaaaatac atttagttat ataattttat
     7681 tttttctaag agtataatta taaaaataaa aaaagaatag gataattttc ctactctttt
     7741 ttctgtactt acatataata ttaaaactct ttatttttta atatatattt taaattttct
     7801 tttttaataa atctttttct tttaataaat ctatttaatt ttcttcttct atatcagagc
     7861 caaagggaat ccatgcatta ccaaactctt tatctttaaa taaataagct aatccagtta
     7921 tttgcttcaa tctaagaatt tcatcaactt ccatacccag gtgctttgat atccattcat
     7981 cagattttcc aatttcacaa agttcagtta taattttact cattaaatcc acattatgag
     8041 aacctcttgc acgattgtgt cgaatagtac ttgccattct ttcatcaaga gatttatcaa
     8101 ttactgaaac aggaagaagt ccattttctc tatcatatat atcctcattt tcaagcatta
     8161 ctctatatct atgaaaacca tctacaataa tatacttatc cttttcctca ttataataac
     8221 agacaattgg cattgtatac ccatcttctt ttatactttt atacaacaac tgcatttctg
     8281 gtggggctac tgcatttgga ttatagtcgt tagattctat cttttcgatt ggcactgcga
     8341 tgattccata tacaggactt ttatattcca tagcatctta actcctatct aatgaatttt
     8401 ttcatatttc ttcttaatat attccgttct aattctctgt tcttttgtag gaccaaatcc
     8461 cataaatcta cacatatggt cattttttaa aatacaataa cacattcttt tccaacttgg
     8521 tatatctttg gtaaatttaa tatcatccgt atcatcaggt atatcatcca aaaatatgac
     8581 tctactcttt ttatcttttg tatgatttga aactccattt aattctattt tataaccttt
     8641 tctcaacaat tcacgtataa ctatttctga caaaccacca cctgtattct gccaaaactc
     8701 aatagatgtt gaaaattttt taatatagct attccttata ttttcaggta aggtatcaag
     8761 caaaaaatat gtgaaacttt tccatgtgtg tccatatggc aatttaacac tacgatatcc
     8821 catagctttt gttttgccat aaatagaagt aaaattaact ccttgtactc tagcaaccaa
     8881 tttcacccat acatctgggt ccaatattct gtataaattt aaactctctt ttgctgaatc
     8941 attaaaaggt gatgccactc tcatttttct tggagctact ccagctttat agaataagtc
     9001 ataaatttta ttatagtcat agtcaaattt atagtgtgct aaccatatat cttctatttc
     9061 ccaatcatat ataggagatg ccgtccagac atttttaaat tccttagtta tccagcactt
     9121 tcctttatat ccatgctgtt tattcacaat agaacaataa cgttgtagag attctgctgc
     9181 tcgtgttcct aataagcaaa caacttttgc accttcatgt atatctctaa tccatctacc
     9241 aaattgtttt gccaaacttt cttgtctcat tttatattta taataagtaa aaggattatt
     9301 tttttgattt attatgtatt ctttgactgg catttccctt acccataact cttctttctc
     9361 atcatcccat ggataccaat acatttcata atttcctaca gcagttcttg tagccattgg
     9421 taaacacacc caatatggtt caatatcttc aatattatct tcaaatactt tttcaacata
     9481 ttcagtagtc aaactatatt gagcctcaaa atcttgatga aatactccta ttttggaact
     9541 tatattattc tttctcttat aatccaaaac taaatttagc agtaaaccac tatcttttcc
     9601 tccgctaaaa gatacataga tataatcaaa ttcattaaaa agataagaaa gtctttcttg
     9661 aaaagcttca aatacatcgg attctaaata tctcttatcc atttgaatta tcccctttca
     9721 attagaagtt tataaaacct tacaatgcaa tattattatg cttttttagc agtaatcagc
     9781 ataaattata cattatttaa atacacaaat ccaactaact acatataatt ataatgtaat
     9841 tatacttttg tattataaac tatattttat cattatttta catttttttc caatatatat
     9901 aaatatcata tttttctatt agctaaaact aattacttta tatttctacg tattagtata
     9961 ataatacata aaaacttgaa taatgcataa aaacttgaca tactgttgtc ataattactt
    10021 tgctataatt aaaagtaaaa atggaggtaa attctaatgt atgaacgtat gcttgataaa
    10081 agcaataaac cctgtttgtc ccaaataata gaatatatag gtaaagatgg atatgcacga
    10141 ttgtgtaagt tggaaactta cttgaaaact caatataatt tatcaagaga acttcgattt
    10201 ccttttggaa acaattatgg ttggggttac aaatatagcc ataaatcttt tcatttattt
    10261 tatgcttttt ttgaaaaagg tgcatttact gttctgattc agataggtga caagcaagtt
    10321 ccagcagtag aaaaaacgtt ggtagctttg acaccaaaag ctaaagaact ctgggtcact
    10381 cgttatcctt gtggcaataa tggaggctgg attaagtatc gtattttaga agatgcagac
    10441 ttacttgata ttattcaata tataagagca aaaaaatctc cacctaaaca aaattaactt
    10501 ttattttttg tgatttaaat atagtgtata taaattccat tctttccata ttaaaaaata
    10561 atataaaaaa tatctattag tataacttat ttagtatgtc ttattctact ttttacagat
    10621 aattaaaaag ttttatcata aataaatcat aactagacat cctagtaaat caaaattaat
    10681 ttattctttt ttattcgaat ttttgataat agtccctata catatgccaa ttatcattcc
    10741 aaaaattaga ccataacaca ttgtaatacc tacatttcct ttagaaatac ctatagcaca
    10801 acctatagcc attccaagta tcatgcctat aaacatacat ataataacta aattgttgtt
    10861 tttattcata gtttttctcc attcaatata ttttaaattg cctagctcgc cttcttagct
    10921 taaaacaaag aaatataaat taactttctt tcattctaat atctgcctgt ataaatgtca
    10981 aacaacacat ttcttgtcat aacctcctct ttttgaagta agtacaatct cccagacgca
    11041 ataatatcaa tttcctaaaa ccatttaagt aacttttata tttacataat atttacaatc
    11101 ataaactata ttgttattca tttatacatc ataaacactc cctcctaatc attattatac
    11161 ttaaaataat gttcatttat aataatatat ctaacgcact ttaattgaaa aaactaaaac
    11221 tttgcctttc attcccatat ataaataagt atactttttg atgctctatt acaaatcaac
    11281 taaattaatt gatataaatt atcattttct gttgaaaata ccattcgcac atgctatact
    11341 taattgcata aatggtaata aatgtcatat gattttacaa atctattaat aacaattatt
    11401 acttttactg tattttaaag tttttggact aaatatcaag aactttctaa aatacaaagt
    11461 acaaaaaatt tatataagtt ataaatttat atgaactaga taagctcaaa caatatgtta
    11521 tgtataaact gtgtagtatt cggttataaa tttatataaa ttatataagc tagataaagt
    11581 attacctcaa ctatataagc tagatatata aattgcttat ataagctaga taagaattat
    11641 ttcaactata taaactacat atataaatta catacataag ctagatatat aaactgcata
    11701 tataagctat ataagaatta tttcaaccat ataaactaca catgtaaaat tcatataaat
    11761 ttactaatat aaatcaaata aaattactaa actctctaaa ctaattttgt aaaacatact
    11821 ctatttttaa catttgttac cttatacata agtacaaaca aaatctaact aaatgaaagg
    11881 ataatgtaaa atgaatattt taaaaatgaa aaaaacaggg atatttttag gaggagtact
    11941 ttttggtact gtaggtgtta agatactttc aagtgattgt gttaaaaaaa cttgcactcg
    12001 tgcaacagca ggagcattga aagttaaaga ttctgttctt gaaacaacta cgaaaattca
    12061 agaaaatgta gaagatattt tagctgaagc aaaagaaatc aacgagcaag aagacttatc
    12121 tacatctaag gtaaacgttg tacaagatac tcttaaatgc gaacaagaag ttcttaaaac
    12181 tgaaactata aaaactgtag aagatactaa ttctaatagc attaaatgtg aagaaatcaa
    12241 agataacgta attgaagaaa aaaatagtac ttgtacaaca gaataaattt aatgattatt
    12301 atgattaata atatcaggag gaagtaaatt gaatttttat ataaaacacg aaataagagg
    12361 tcgtatacgc tttgacttac aacttggcaa attaaacaac aagcagtccg atactctctt
    12421 atattattta acttctataa atggtattac caaagcaaaa gtttacgata gaactggtga
    12481 tgcagtcatt tattacactg gtgaaagaga atacataata agagaaatca ctaaattcag
    12541 ttttaacaat agcaaaatag aaaacctagt tcctgaacat accagcaggg aattaaacaa
    12601 ttattataga gacaaattag caaataaact gctagtcaaa agtatctcca aaatattttt
    12661 tccaaagtcc ttaagatttt ttttaattgg acttcattct ataaaatatt ttaaaaaagg
    12721 tctctcttcc ctgctaaata aaaaaataga agtttctgtt ttagatgcca ctgctatagg
    12781 tatttctatt tttagaaaag atatcaatac ggctggttca gttatgtttc ttcttggtat
    12841 cggtgaacta ttagaagaat ggactagaaa aaaatctata gacgacttgg ctcaaagtat
    12901 gtcactaaac attgaaaaag tatggttaag aaaaggtgac actgaaatct taattccaat
    12961 ttcagagata aaggaaggag atttagtaag tgtaactatg ggaaatatga ttccattgga
    13021 tggagttata gtatcaggag aaacaatggt caatcaggca tcacttacag gtgaatccct
    13081 tgccgttaac aaaaaagaag gctcttacat atatgctgga actgtaatag agcaaggcaa
    13141 tattgttatg tgcgtcaagg aaaaagctgg tactactaga ttccagaaaa tagtaactat
    13201 gattgaggaa tctgaaaaac taaaatcttc tgtagagagt aaatttgaac atctagcaga
    13261 tacattagtt ccatacagct ttttaggaag tatattaacc tatgcaatta ctagaaaccc
    13321 tataaaatca ctctctatat taatggtaga tttctcttgt gcattaaaac tctctatccc
    13381 tatttcagta ctatcagcca tgagagaatg taacaataac aacattactg taaaaggtgg
    13441 aaaattcctt gaaggtgttg catgtgcaga tactattgta tttgataaga caggcacatt
    13501 aacaaaagct caaccaactg tttcagatat aattacattc caaaattata ataaggagga
    13561 tatgttaaga cttgcagctt gcttagaaga acactttcca cactccatag caaatgcagt
    13621 agtttacgaa gctgaaaaac aagggcttag tcacaaagaa atgcatacag aggtagaata
    13681 tattattgct catggcattt caacaaaagt tgatgataaa aaggttgtaa taggtagcaa
    13741 acattttgta tttgaagatg agaagtgtac tatcccagac ggtgaggagg ataagtacaa
    13801 caatctatca gatgagtatt cacacctatt tatggcaata tcaggtaaat tatcagcagt
    13861 tatttgcata aatgaccctt tacgaaaaga agcaaaatat gtaatttcaa atttaagaga
    13921 atgcggaata aaaaagattg tgatgatgac tggagatagc gaaaaaacag ctaaatctat
    13981 agcaagtaaa gttggtgttg atgaatatta ttcagaagtc ttaccagaag acaaagccaa
    14041 ttttgttaaa agtgaaaaac tcaaaggtcg caaagtaatt atgattggtg atggaatcaa
    14101 tgattctcct gctatttcag aatcagatgt cggaattgca atgagtgaag gtgctgaaat
    14161 agcaagagag atttcagata ttaccatatc agcagataat ttgaataatt taatcgtttt
    14221 aaagcagatt agtaataagc ttatgaaaag agttgattta agttctaagt ttattattgg
    14281 ctttaattta ggtctgattt tacttggtgt aggtggattt gttagaccaa gtacatctgc
    14341 ctttttacac aacacatcta ctgtcggaat aagtttaaac agtatgacaa atttattgga
    14401 aaacaccaat acttataact accattaatc tccattacat aaaacatatg atataaaaaa
    14461 tttataattc atctttttaa aagaatatgt cttaaaatta gcttttttct aatcaaaatt
    14521 atataaagca tactttacaa attttaaact caacttattt ctatttttta caaaaaagga
    14581 gatgattcaa attttcattt tgaaacatcc ccttttgtaa atttctttaa aatttatata
    14641 ttaattatat tttcaaccaa taattgtatt ttcaaccaat ctttcttcat agcattttaa
    14701 atcattttgc tttagataat taaattcaga ctctacataa ataccacagc ctaccaattc
    14761 aattgtcaaa ttaggtatat tttcctttag tatactagtc tgctcataaa taattctttc
    14821 caataaatca acttgtaata tagaaaaatg cttcataatc tccccatcac tatctatatc
    14881 tttcttgatt tcataccaac tctttgacaa aattcccttc aaatcaatat accctagact
    14941 cctcaatata tctatactat cgctccatgc atacttacta ggtttatcaa agtatgattc
    15001 tagtttcttc ttctgctcat ttgtaagtga atcataagaa agcttcttat taaaatgtgc
    15061 cttcaaagaa atcttagcca tagaatatgg acttctacca gagtttaatc ctgcattttt
    15121 gaacttttca attcctttat cttctactga agaccatatt gagtttattg aatctgaatc
    15181 attgtattta atatcaagac catgtgtaat atttatatag ttttgtggaa atacatattg
    15241 ataatagaca tcttttttat catcatatag agagaagtat ccactatcat ataaatgaat
    15301 atgcaccttt acttttaaac tatcctctaa aatatttcgt ctaaggttcg ggatatttgc
    15361 atacatggtt acatatttta tggcatcaaa gtttttattt aatggaactt caaaactaat
    15421 ttcatctcct gtcaaaagct ttttcacttc ttcttcatta caatcatata gagaatatgt
    15481 attttcaaat cccacacgtg atttcccatc taaactgcct gttaatgcta ccttcaaaat
    15541 atggttatct gttttttgcc attggtaagc atcttggtca agaaactctc taatctgtct
    15601 attttgctct ttcgcctgat ttataacctc gttttcatct tctgtaatcc ctacatggca
    15661 tctatttaag atatttacta tattgcttcc aaatgggaat gtccatagtt gaaaagctgc
    15721 tgcctcaacc tgacacaaat atctaacctg atttaattca atttgatgga taagtttttt
    15781 ctgatattca ttcatctgat taccactatc tgacatttga taaatcaatt ctcccattgc
    15841 aagattatct tttttcttat caatcttatc agtaatgata atttgagttt ccatcatatc
    15901 ctgtactttt ttaaatatta tagaaatctc ctctgagttt tccattttta agcttccaaa
    15961 cttcgtgctt aattcagtta aaacttcctt catctttaat ttacttatct cattttcaaa
    16021 aatatcttga tttttaactg tactatcgaa tttttctaca aatggtataa cagattcttt
    16081 caaagtttta tctttaaatt ccaccatttg agtattcaat tcatctaatt tcttaatatt
    16141 attttcttta tctttaattg cctctttaac tacctctatt ttactctcaa aactagattt
    16201 tacattaaga caattattaa caccactaat cgcagtattt ataagagaac aagcagaact
    16261 tccaatactc aatgcatttt taattttaga tgtcttttgc tgtctttcaa tagcttgttt
    16321 acttttctct agtgcagcta acttatcttt ttgctgttta ttattgtaat ttgcctccaa
    16381 catacctatt tcttttttct ggatttttgt gtaaatttcc ttatactcgc tttcacttaa
    16441 cccctctttt gctctttgaa tatcgtcagc tttgtctgca tagttatcat catctataga
    16501 cttaactata atctccttaa tttggtcaaa tttagttctt ctttcttcat caacacctgc
    16561 caaattataa gtcattttat ctaaagcatt ttttctatct tcacttagct gttctccaag
    16621 tacaaatctc ataccatcag aatccaattt aaaatctgaa aaatcaacca tcctgttagt
    16681 ccattggtat tcctcttctt tagctgaaag attttccttc aaagattttg cactgtcaaa
    16741 tgcccctaga caagcttgac caacaccact aaatgcagtc agtaaactca atgcgctttg
    16801 tattccagaa agaattaact gtgttttcat ttgttcctca agttttgcct gtttcttttt
    16861 tagttcctct atgtcttctt gttcatcttc tttcatcttt ataatttctt gaatcaacag
    16921 gttcattttt gcattaacag aaagcatttt attctcaagt tcatttttta aaacctctat
    16981 ttgttgattt ccagattcaa tttgagttag gatactttcc ttatatgtat tgaaatatcc
    17041 ttcttttaac tcatctttct ttccttgttc caattctctt aaggtcttat ccagatagtc
    17101 tgtcaaagaa cgaatatctg taacacgatt tcctccaatt tcactttctt caatcatctg
    17161 cacataaaat gctgtcttct gctcaagtgc aaataaaatc atactccatt cgctaaaatt
    17221 ctgatttttt cctacaaaag attctattct atcagactgc atcaatttat ttataaggta
    17281 ttcatagatt tcctttgcct ctttataaaa ttctttcttt atattcatgt catccataga
    17341 aagtactgct ttatttttct tctcaaaact atagtacttc tgaagtattt ctctatttaa
    17401 ttgcatctct attttctcaa ctgcataaaa aatttcataa atatgtgaaa attctaaaat
    17461 atcagcttct aaaattccta ctgcacacaa cagtctaaga tttctataac ctttaattcc
    17521 agaaaaacca aatctctcga aaaattcata gaagaatgta tctattgaca aagaatactc
    17581 ctgctttaac aattcctttt cttttagtgc atctccagag tgaccggctt ttccctctgc
    17641 tccggctgaa cctgaatctt cacgtttgct aattgtccag tccttatgtt cttcataaag
    17701 agtatttaat ttctctccat catctgctgc ttgcttctga tttaaagcat gattaaacac
    17761 atatctatca aaataatagt agtttctatc actattatcc attggaggag aggtacctat
    17821 atatcttatt gtttcttcat atatacctct aattccacca agacctcctg caccaccagc
    17881 tcctccagag cctccttttc ctgctaaacc atgatttcca tcttttttac ttatagcact
    17941 ttttaataaa ggagcttttc caaggagata aacctgtcca gacttgcttc caagacctgc
    18001 atttccacca ttacctcctg tgcctccagt tccaccttgt tttccttttt ctgcttttac
    18061 aacatagtat tttgtacctt taacattagc aaattctttt gactcttttt tagaacctat
    18121 tttagcattt gccccagctt ctccagttat ccctgtatct cctttttttc catcttgtcc
    18181 atttccacca cgtccaccat ctacgtaaag tttaagttct cctctttttt ctatttcttt
    18241 aaaagataat atgatatcac ctgaagtacc tgcattattg ccatttcttc cattcattcc
    18301 gtcgtaaata gaactttctc caaccaattt ttctgcatcc ttaccagata cgttgattga
    18361 ataagtccct tctggtatat gtaatttttc tgtaagtagt acaatattct tacctgagaa
    18421 gacttctttc tccatattac agtcaatttt caactcacaa tctccataca tatatataga
    18481 tgacaaaata ttaagttgat aaccagatat tattttagtc aaatccgata aattcacttt
    18541 aggtatagga atatatatag aaagtacatt ttctttctca tatgatttta tatatgtaca
    18601 actttgaatc ttctctatcc tatactctgc atcttcaagc tttttggctt cttcttccct
    18661 atattcttct tctgctatcc aatatttgat atcttttaat gtatcttcta aagtattttt
    18721 tagcaatgat tttaacaact cacttagacc aacaatatga gtctcactac agtaactatt
    18781 taactctaaa agaagacgtg tttcttctaa tatattcact ttctgtacgc catcaccata
    18841 tgaaagttct tgtacaaact ccataagctc tttcatatta ttagtcctac tacataaatc
    18901 tgtatacatc ttatctaatt gaatcttttt atcttttaga ctatttatag aagatagaat
    18961 ggaaatagaa tcttccatac cttttttaac actttctata taaggaacta aaagtttttc
    19021 taggtcttta taggttacat caacagcttt ttgtatttct attttacaag aagctgtaag
    19081 catcaatccc aaatctgttt tttcaaatgt ttcaggtaga tttttcattt catccaacaa
    19141 cttttctggc tgtttatcta gtggattaca taatttaata gatacaccat ctgataatgc
    19201 tacatttaat aagtacacta attccttaca accgtcgtaa tctttctctt gactatctaa
    19261 gtcttctttt gctttttcca atatatttct tattttttct tctgttacat taccgattag
    19321 tgttttaggt attttattaa taagaaagaa gaaattttct ttatactttg cataatcacc
    19381 aagcatatct ttcaaatttt tgcctgtttc tatcaataat gtatgtctag agtccagtag
    19441 ggattgatag tctaagataa ctaccatccc tttcaccttt gaagatgtgg aaatagtttc
    19501 ccttacaagc atagcaccag caatcatata ctctgctcca cggttgtcaa aaaaacctgg
    19561 gcaatcacat aatgaaaaat ctctatttgc aatatctaca acttctggac ataaagtctt
    19621 agaatataca ccatttccaa tttcgacagc tgaatctttt gcagttatat atatttgtcc
    19681 agtctcttca tccgtttttt cttccatcac acagcctttt aaataattta tacaagtact
    19741 ctttccaacg cctgaagcac ctaggaataa caaaattgac ttttctttta agtcatattt
    19801 tctaagctcg tctcttcctt cttccaaaag gtttacgatt tcactataaa gtttatttgc
    19861 catacatcca tccctttcta tatttcggaa caatagcctt gatatttttc tttaataaga
    19921 ttctcttcta cctttgaaac agctgttgat aacttagtat ttggagcatt aaatcctcca
    19981 ttgtctcctc tatctccata ttcagcgtat tctcttgctg gtcctgtttt accttcattc
    20041 cagcttccac cactaaattc tatatgatat ttaattatac tatctaactt ataatcccaa
    20101 cttgtaaaat atcctgtata gtatacagtc ttatctatac cacttggaga aaatgaaagt
    20161 gtattatatc cgtgatagcc accacttcct ccatatccac ctaaacctgg tctaccattt
    20221 ggacctgctt ctccatcttt cactgttata tcacaatgat taattattct atttttttgt
    20281 tcctctgaat ctgaaatgaa gtaaatatta cctttcttac cagcatttcc acctcttcca
    20341 gcatttccac cagctccacc tttttcacct tttttgccac ttgtaccttt ttcaaaatac
    20401 cctttaaaca tctgtccgtg gaaataactt gtatatcctg catcagctcc attacttcca
    20461 gaagctcctg tttcaccatt ttctccatct attcctttag aaccatttcc tccatttgca
    20521 atcagtgtta gacgataatt tctaatttct tctttaatat ataagtaaat attacctgca
    20581 tctctaccat cttcaccatt tttcccatac atagaattat ctacaagttc tccgctcttt
    20641 ccacttacat taatttttac attactacct acatcaagat tgttagaagc aagaataata
    20701 tttttctctg atgcaaagtt cattccaagg ttatcatcca tataaatagt atctttagca
    20761 aagaacataa aagtagaaac attgtcatct acttttctat ttttgaaata ttccaataca
    20821 ctgctaatta ctaagtttgg aattaatgct cttatttcaa tacctttctt atcattaata
    20881 ttaattgttt ttactataaa tggatttaaa atgtatttac caataaattc tctaattatc
    20941 tcattacctt cttctccata tgcacttaat cttgttatac tgtcttctaa ctcttcattt
    21001 tgtgtaactg taacatttgt caaaattgaa ttaaaatcac ttacactata tacataatcc
    21061 tttttatttg caacttccat taatttttct ctatttggta atgtaaatac ctgtttttgc
    21121 acttcataag aaactaaaac tttttctaaa ttctttctta ttataatgaa gttttgttcc
    21181 attatcttct tacttgcctt ttctataata aattccatat gtacagaagg taataaaatt
    21241 tccccagttc catgttctgt atatttactt aaagcatcaa ttatatctaa gtttcctttt
    21301 atcttatttt gtaatttcat gcaactatct tgtaagaata atccactttg tttggctaat
    21361 acgttgataa aactctctat agtaagactt gttgtacttt ccaaccagtt tttaaatact
    21421 tttacaacct catcatattt tgctatttct tgaatcattg ttatacttcc aataatattg
    21481 ataaaatctt ctgtatacgc cttaacataa tcttctaggc tagatacgat attgttttga
    21541 atttctttta tcaacgtatc taaaactaat cttgcatctg cactaattgg atattggaat
    21601 gctgatgtct tatctttaac ttcatctaat tctagtactt gctttaataa gccttccctc
    21661 tcatctgatg caagtggctt acaaagatga atattttttc cattatcttt tagcaattct
    21721 tcaaataacc aagctttttc ctctatatta ctgtctaaat tttcgcataa ttgttcatat
    21781 tttcttttta taacattata tatttgtttt tctgttcttc ctaagaaact ttttgaaatg
    21841 aaaaaagtta tactaccctt aaaattttct ttatgaagac acatatttaa ctgttggaac
    21901 aggtctagta cttttgtaag tctagaatct aatatatctg cttctggtat caatacaatt
    21961 actgcacata tagatgcaga atttttaaat acttcaccta atgaaactgc tgcactaata
    22021 tcatgggcta ctcccctatt gtcgctaaaa cctgcagtat cacataattt tgcatccatt
    22081 ccttctattg gttttctcat atttacaatg ttcgggtata aagtttgaga aacagctcct
    22141 gaaccaatct ctgccacagg gtctaagcaa tcaatatatg atataccagt atcttcatct
    22201 tctacttcca tcattacaca tcctgcaaga tagttaattg cagtactttt gccacatcct
    22261 gtcccaccaa gaattagaat taaattttga ttttcagcct tatttattgc tacttttcct
    22321 tcatttaata aaagtgttat ttcttctaaa agttgattgt tattttctgg catttatttt
    22381 cccccttggt tatatatttt atttaaataa agtttaatcc cttaagtaga ttgcaaagtt
    22441 tttagactat atttaaattt gttttttatt ttttattttt aataaatttg cattttcact
    22501 aaataattat tcttctttgt ttattaaaat aactgtgttg tataatataa tctttatcaa
    22561 cacagttatt ttatttaatg cgcttatttt attgtatttt atgtaataca gttgtttttt
    22621 atattatatg tccattattt ttctatggtt attacatata gatttttaat tatatttgta
    22681 ccattaattt aaacattcct atactactgc ttaagtttta cacctatacc agcttgaata
    22741 ttttattttt atttgcttaa atagttcaac tatactggtt taaataggtt atctttattt
    22801 gcttaaatag ttcaactata ctggttaaat aggttatctt tatttgctta aatagttcaa
    22861 ctatactgat ttaaataggt tatctttatt tgcttaaata agttcaacta tactgattta
    22921 aataggtttc ctttagttta aataattcac ctatactggt ttaaataggt ttcctttagt
    22981 ttaaataatt cacctatacc tacttgaata gttcactttt atttgcttaa attctatacc
    23041 aatactggct taaacattat actaccatga ttgtattgtc tatcttcaac tacaataaaa
    23101 acaggttcta aatctttagt agatgttatt tttaattcta aaaaaatatt atcagttggt
    23161 aatattttta catcataatt atttaaatta ttattcgtga tactattaaa tacatcacct
    23221 tgacgttcaa aaaaaacatt atcaactatt acaaaataaa catcttcttt tctactattt
    23281 tcaacctcta tagtattttg tttagttata tttagtctca tcttccacct cctattaata
    23341 ttacttaatt atattaatac ttgtaattgt tgcattatgt tcatcacaag gaagaggttt
    23401 aaaaacaact atctctgcta aataattctg tggaggattt gtaactgaag tagcattgca
    23461 aataaattta aaatttgttc ctgctacaac ttgagtagaa acaataagtg gttcataacc
    23521 aaccccttct aacattccta cagcttcatt aaatactttt aaatcttctt cttcgacagc
    23581 tctgaatttt gaccatccac caacaaaaag actcatataa aaacctcctt tatatatata
    23641 tttatttata tatagtagta ccaattttct caatattcaa tattttctgt gtaactggtc
    23701 aatgaaaatg cataactgga taaaaatact tataaaataa cttaacttta catataaata
    23761 gcttacttta catgtaataa gattattcta catgtaataa gattattcta caatatgaaa
    23821 tgcatcttgt tttgaatata ttctaaatac aataatggat attttcaatt attataatta
    23881 gaaaatatcc attacttaac ttgttttatt taaaatcaca tagagacttt attatttttt
    23941 ttattgcatg aatagctatt tcaggtatca taaggtgata taccccctcc ttgaaaccac
    24001 aaaaaacatg acaaacttta cgaatcttgt cttaaaaaac tacaggtaca ttccttattt
    24061 gtggattgga gtagccataa caagcaaatt ttacaatttt tatgatgaca aaaatcaata
    24121 aaatcgctta gtatataaca cgaaatacta cttgttacta ttaagaatag ttagatagga
    24181 agtatgcatt atacaaataa aggaatcaat aaagaaagtt aaaacatccc tacaaattaa
    24241 aaatctgtta catttttcta tatttaaacg ttatttactt acaattggag aaataacagg
    24301 ttttccttct cggtcaagta aaggcgtaaa tccggctgca ttgccataat agtggaatac
    24361 atagttcaca cctgtttctt tatccaccca aatctccgtt gcatttattt tcccttgtga
    24421 atatactttt tcaaatctat cttttcccat tttataatcc tccatatttt tgattggtct
    24481 atgccttctt cacttaagta gaaaaaatac ataacttatt tttttaattc taatatgagc
    24541 acgtaaaaat gtcaaacagc ttatttaatt tcataacttc atgttactgt tcgtaacctt
    24601 gtctgtttac ttacactgca ttacagcacc attttatcct ttacattatt gtattgcaca
    24661 cagtaaaaat gcttacttta tgggattttt caactaaaaa aaggatttta tctccacaaa
    24721 acatttacta tttgttacaa tatacctaat tttaatctca tatcaacatt taatgaagat
    24781 ataacctttt tcaaatattg taaagactcc ttaaaaatat tagaaaaaga ttctgcaaat
    24841 ctctcaacat ctcctttttt taaataccta agtatctcaa aacatacatc aaaacgtgtt
    24901 ttcaaaattt cattatattc ttcatgaaaa aatctctcta aatagattcc aaatatcata
    24961 tattgtttta aatgcaaaaa tgtttcctgt aatacataaa tttgtgtata atctactatc
    25021 ttacaaagta aaacaaaatc tggataaacc ttacactctt cacaattatt ttgtaagtac
    25081 cttaaatgag tttctatttt atctacagct atcaaatcca ttttatttcc tagtaaaagt
    25141 actacatcct caagagtaat cgtaacaaac tccatagcat ccaaaagcaa actcaacctt
    25201 ttagctcctt cttctgtatt tatataatca tcatcatatt cagaaaaaag cgttacatat
    25261 gttcccttgc cattaattgt tttagcaatt cccaattcat ttagctcatt ataggcactt
    25321 ctaactgttg gaagtgatac attatatcta ttaattatcg aagtaattga tggtagacaa
    25381 tctcctattt taatattgcc ttcaaaaatt tgacaaatta catctgaaac aattaagtca
    25441 tataaataac atttttccaa cgttacagaa ttaaaactac ttgtatctat actttgtgga
    25501 gcacgatttt ctaccatttt aagtatggat tctctacaag ctttatacat atatgttaat
    25561 agttttttaa attcaattag attcccatcc aatccatata agtaagtttt tcttacctgc
    25621 tctatgtatt tattacgaaa aaagtctata caacaattat ccaagttatc caagttatcg
    25681 aaattatagg ccaattgtga aataatgaca tcatgctgaa taatctcaca aaaatggtga
    25741 atcaggaaat tatttgtttt acttattagt ttatttatat attttagcct ttgttcttta
    25801 aagactaaat aattagaact atttttttcc atacaatcga taatctcgtt taattcttca
    25861 aagtctttag atgtaaatct tttaatattg tacatagcaa tagaaggaaa tattaaacta
    25921 aaaatcttat atatttctga tatttgtaaa atcttctcat ataataagtc agattctatt
    25981 gattgtttac tcgtactctg gattaaaact ggtctagaac cagcttttgt ctctataaag
    26041 cccttttctg agagcatttt tattacagtt tttatagtat tttttgatac catatattct
    26101 tctgacatgc tttttataga atcaagccta tctccaatag taattttacc agtataaatt
    26161 tgttgaatta tatgctcaaa aataattaaa tattgtggtt ttctcttcat atttttcctc
    26221 ttcgataaat tagatttatt gttttttgta aggtatattt acttttattt aatagggcaa
    26281 aatagttgct tattttttcc tcataatcta taattaatat aaaccattta tatatatggt
    26341 ctaagtttta taatactgta atgcagtaaa atagcatgtt gtagctatac tacagcaaat
    26401 taacatacca ttgtagtata ttaattggaa taattttaag tacttcaaaa ttccagcaaa
    26461 acatatatgt gtttttgata ttatgtatat tcgttaatag aatagctata cttgttttac
    26521 ataataaatt atatgatact tgctatatgt aatcactata tttaatacac ataactatta
    26581 tacttgctat
//
LOCUS       DAYAOX010000032        26090 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.032, whole genome shotgun sequence.
ACCESSION   DAYAOX010000032 DAYAOX010000000
VERSION     DAYAOX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.032"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            158..541
                     /locus_tag="ACGI57_001078"
     CDS             158..541
                     /locus_tag="ACGI57_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFG9716164.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            543..893
                     /locus_tag="ACGI57_001079"
     CDS             543..893
                     /locus_tag="ACGI57_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716165.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(994..1452)
                     /locus_tag="ACGI57_001080"
     CDS             complement(994..1452)
                     /locus_tag="ACGI57_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716166.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLEEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(1497..2285)
                     /locus_tag="ACGI57_001081"
     CDS             complement(1497..2285)
                     /locus_tag="ACGI57_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9716167.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQEGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(2424..2861)
                     /locus_tag="ACGI57_001082"
     CDS             complement(2424..2861)
                     /locus_tag="ACGI57_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HFG9716168.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            complement(3095..3229)
                     /locus_tag="ACGI57_001083"
     CDS             complement(3095..3229)
                     /locus_tag="ACGI57_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716169.1"
                     /translation="MGILEGAAMIREIAIKIAKEKGITEQKAWPEAVKEFKEKYELVL
                     "
     gene            3683..4192
                     /locus_tag="ACGI57_001084"
     CDS             3683..4192
                     /locus_tag="ACGI57_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9716170.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            4219..4365
                     /locus_tag="ACGI57_001085"
                     /pseudo
     CDS             4219..4365
                     /locus_tag="ACGI57_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            4598..5134
                     /locus_tag="ACGI57_001086"
     CDS             4598..5134
                     /locus_tag="ACGI57_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFG9716171.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(5561..6361)
                     /locus_tag="ACGI57_001087"
     CDS             complement(5561..6361)
                     /locus_tag="ACGI57_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9716172.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(6556..8154)
                     /locus_tag="ACGI57_001088"
     CDS             complement(6556..8154)
                     /locus_tag="ACGI57_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9716173.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            8482..8658
                     /locus_tag="ACGI57_001089"
     CDS             8482..8658
                     /locus_tag="ACGI57_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716174.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            8832..9962
                     /locus_tag="ACGI57_001090"
     CDS             8832..9962
                     /locus_tag="ACGI57_001090"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFG9716175.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            10203..10553
                     /gene="eutS"
                     /locus_tag="ACGI57_001091"
     CDS             10203..10553
                     /gene="eutS"
                     /locus_tag="ACGI57_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFG9716176.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            10561..11010
                     /locus_tag="ACGI57_001092"
     CDS             10561..11010
                     /locus_tag="ACGI57_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFG9716177.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            11112..11687
                     /locus_tag="ACGI57_001093"
     CDS             11112..11687
                     /locus_tag="ACGI57_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9716178.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            11680..13089
                     /locus_tag="ACGI57_001094"
     CDS             11680..13089
                     /locus_tag="ACGI57_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HFG9716179.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            13273..14706
                     /gene="eutA"
                     /locus_tag="ACGI57_001095"
     CDS             13273..14706
                     /gene="eutA"
                     /locus_tag="ACGI57_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFG9716180.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            14724..16088
                     /locus_tag="ACGI57_001096"
     CDS             14724..16088
                     /locus_tag="ACGI57_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HFG9716181.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            16101..16982
                     /gene="eutC"
                     /locus_tag="ACGI57_001097"
     CDS             16101..16982
                     /gene="eutC"
                     /locus_tag="ACGI57_001097"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HFG9716182.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            17002..17655
                     /gene="eutL"
                     /locus_tag="ACGI57_001098"
     CDS             17002..17655
                     /gene="eutL"
                     /locus_tag="ACGI57_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFG9716183.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            17666..18367
                     /locus_tag="ACGI57_001099"
     CDS             17666..18367
                     /locus_tag="ACGI57_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFG9716184.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            18370..19839
                     /locus_tag="ACGI57_001100"
     CDS             18370..19839
                     /locus_tag="ACGI57_001100"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HFG9716185.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            19935..20222
                     /gene="eutM"
                     /locus_tag="ACGI57_001101"
     CDS             19935..20222
                     /gene="eutM"
                     /locus_tag="ACGI57_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFG9716186.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            20349..21110
                     /locus_tag="ACGI57_001102"
     CDS             20349..21110
                     /locus_tag="ACGI57_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HFG9716187.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            21123..21752
                     /gene="eutD"
                     /locus_tag="ACGI57_001103"
     CDS             21123..21752
                     /gene="eutD"
                     /locus_tag="ACGI57_001103"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization phosphate
                     acetyltransferase EutD"
                     /protein_id="HFG9716188.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            21794..22519
                     /locus_tag="ACGI57_001104"
     CDS             21794..22519
                     /locus_tag="ACGI57_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HFG9716189.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            22532..22804
                     /locus_tag="ACGI57_001105"
     CDS             22532..22804
                     /locus_tag="ACGI57_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFG9716190.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            22797..23348
                     /locus_tag="ACGI57_001106"
     CDS             22797..23348
                     /locus_tag="ACGI57_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFG9716191.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            23360..24451
                     /gene="eutH"
                     /locus_tag="ACGI57_001107"
     CDS             23360..24451
                     /gene="eutH"
                     /locus_tag="ACGI57_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFG9716192.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            24453..24926
                     /locus_tag="ACGI57_001108"
     CDS             24453..24926
                     /locus_tag="ACGI57_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFG9716193.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(25046..25141)
                     /locus_tag="ACGI57_001109"
     CDS             complement(25046..25141)
                     /locus_tag="ACGI57_001109"
                     /inference="COORDINATES: protein motif:HMM:NF012392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9716194.1"
                     /translation="MGYPNVEHFIRLFNKKYNMSPSKYRKNTSKI"
     gene            25442..25963
                     /locus_tag="ACGI57_001110"
     CDS             25442..25963
                     /locus_tag="ACGI57_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9716195.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
BASE COUNT        10271 a         3247 c         4332 g         8240 t
ORIGIN      
        1 atatatagaa aatccttata aaaatttaaa ttgtaatgaa taggaagtaa ttaaaaagaa
       61 tttttattga tttttaaaaa tttagttaga aactatatca ttatggtaaa aaatgtatta
      121 taatgtttag aaatattata ataaggagcg attaaacatg aatctatcga agaaaataga
      181 agataaaaga aaagctaaac agagagctga aaaagttaag aaagctaaaa ttgctacagc
      241 tggtgtggta ttaggagctg ttactggtgc agtgagtgga gtacttttgg ctcctaaatc
      301 tggaaaagaa acaagagagg atataaaaga tgcttcacaa caaattgctg aaaagataaa
      361 tatgaaaact gtagatgtaa aaggcaaagt atctgagaag ctagaggata aaaaaggaaa
      421 ctttatagaa tcaaagaaaa aaataaaaaa atatatagat aacaaaaaga gtgttgaaaa
      481 ttctagtgag gaagataaaa taatagatat agttgaacca gttagtgtta ctgaggaata
      541 atatggacaa tacaataacg attaacataa gtataatata tgtattatta gctatacttg
      601 cttgtattgc tttagtttat ttaattattg tacttgttaa actacataag attttaaata
      661 atgttggaac tatgttaaat gaaaatactc aaaatataaa tacaacttta aataatcttc
      721 ctaatgttgt agaaactttt ggagaagtag gagaaaatat gaaagatgta acagaagtag
      781 ttactgaagt tacagcagat tttcttgtta ctaaagacag tgtcaaatca aatatggatg
      841 ttatagttga tatattgact ataattaaaa acatttttct agataaaaaa taataagttt
      901 aattaaaata agcctataag atataattta aacgacctta aaatagcatt tataaggtcg
      961 ttttttatta gcttgatttg acactatgtg tttttaataa ttggtaatat tcctctttat
     1021 catttgaaca tatatatcta tgcttgttgt ttcaattaca tccataagaa catctttaca
     1081 ttcaagctca aaatcttcca ttccttctaa atattcatca tcttcatcaa aaaagatata
     1141 agaattatat agccattctt caaatttttc ttcaagtata tcccaatacg aactccaaaa
     1201 taatccacaa cctgatatat aagatgctct actttgattt atataaacct cctctagaag
     1261 cttttgtaat ctactgctat aaagaaaatc tgctcttcca ctgcttgata ttaattcatt
     1321 taaatcttct attgacatta attcaacttt accactttta tcataaggag tataattttc
     1381 atcaacccat ttatgtggtt tgttttctct gcatactgtt gctatcaact ctacaacttt
     1441 tgttcttatc atttttttat cctaacttat ttatttttta taaaataatc atgattttat
     1501 aatttatcta tttcttgtaa tgctaattta acagtttcat atctatcagc accatttatt
     1561 actacatcac cttttacaag tttattagct tctccaccta caacataaac ctttttacct
     1621 aaaccttgct tataactaga cgcttcaatt attaaagaat ctttcaattg ccaatgtaaa
     1681 atctgagcag ccactttatc agcatcacct gtataaacga ttacattttc atacacttta
     1741 ttttcccctc cctcttgaga atttgatata gatttgttta gtataccttc tgctatcaat
     1801 ttagcaactt tatctttatt attaacataa taatctgtat ctgctttact atctacaaaa
     1861 catacttcta ttaatattgc tggtgcaata gtatgattaa gccaataaag acctctaaca
     1921 tcagactttg cgcccctatc tttgaatact gtagctaacc tagtattcac tctttctgca
     1981 taggttttgc cattattagt cttatatatt gtttctgtcc ctactggagt tgaagttgtg
     2041 gcatttgaat taaaatgaat ctgaacagcc aaatcagttt cctgtttatt tgctatagca
     2101 cattggtctg ctaaatgatt acttgattca tcaactcttc ctgtataaac agtagctcca
     2161 cctattttaa gccatttaac aattaagtca ttcagtattc ttgtttcttt gctttcgttt
     2221 atatacccaa cagctcctgt accttttcca attaaagtgt gccctggtat tattactact
     2281 ttcaaactaa catctccttt acaattaaat ttaacttcta tttaatatat gcataaccta
     2341 gaattttgtt tctttttata tcttatactt ttattataac ccattttgta aaaatataga
     2401 atattaagta aataatgtta aaattataat tttgtatata agacattact tgattgcaat
     2461 aattttatag catattcatc tattctatat ccagttctat aatatatatg tttaattcca
     2521 gcttgaatta tagccttagt acaatttaag caaggaaagt gagttacata aatagagcaa
     2581 tttttaactg aaataccttc ttttgcacaa taataaaggg cattttgttc agcgtgaatg
     2641 gttctaatac aatgtccatc tctcatttca tgaccaactt cactacaatg cttatctcca
     2701 gaaatactac cattatatcc agtagacact attctgtttt ctgaattgac aattatagct
     2761 cctacataag ctctatcaca agttcctctt tcagctactg tttcgcacag tctcataaaa
     2821 tattcttgcc atgagcatct attttcaagt tttttattca aattatatac ctccaaaact
     2881 tatatctaat atataagatt tataccttaa aaataatatt aaaaacaaat ttatacgata
     2941 aataaaattt attaatactg tttttagtct aaattatttc attttaaatt aaagttacaa
     3001 ggaatctaaa attatatata tctttcttcg taatattaat gttacaatat aaaaaactgt
     3061 ctcaataaaa tagagacagt tttacagtca aagtttataa tacaagttca tatttttctt
     3121 taaattcttt tacagcttcg ggccatgcct tttgttctgt aatacctttt tctttagcta
     3181 ttttaattgc aatctctctt atcattgcag cgccttctaa tattcccatt tttaatctcc
     3241 cttcaaatat ctttttgtat agaatataaa tagtgctaag atttattaca tgctgcatat
     3301 acaatatcta tacaacacat aacttgtaaa ttccttttat tttcatacgt ctttaaccga
     3361 catataatta tattcaataa gttttaaaat tatgaactca attataatta tttttatttt
     3421 tttagaatct aaacactaaa aattgtaggt aaatttgtct tatgccttta taaatatttc
     3481 tacagtttta attactggac atatctattt ttattataaa aagcgattaa aaatcgagaa
     3541 tatccgcata aaaactgtat ataagcaaaa cggttaaagg aaaaagaaga aatttacttg
     3601 aatgttaaaa tgcgttgctt gaagcaatgg gcagttttgc ctataatatc ggtaacaact
     3661 gaaagaaagg tggttagccc taatgttaag cgaaaacatt aaaacaatca ggaagtcaaa
     3721 aggactttca caacaagaac ttgctataaa actgaatgta gtgcgacaaa caatatctaa
     3781 atgggaacaa ggactgtcag ttcccgattc tgatatgttg atttccatat cagaggtgct
     3841 tgaaacgccc gtaagtactt tacttggaga aactattgtt gagtcggaag ttgataactt
     3901 aaaagtgatt tctgaaaaat tagagattat aaacttgcaa cttgcacaaa gaaaaaacat
     3961 gagaagaaaa atgtttcatt ggttatttat tttattatgt gcagtcatat taatagtttt
     4021 tgtggtctta gggggactaa atagtcctta cttggattgg aattataccg accctgaaac
     4081 cgctgttttt ggagtagctt ttcatgcttt tgagtggcta tttgtcagat tagtaccgat
     4141 tgtccttata ggagcaatta ttggaatctt tttgacacgt aagaaaatat gaagtaactc
     4201 tgctttttat aatacagcaa gcgttcctta aaagcgtcat tctacatggc aatcgaggac
     4261 gactatgtaa ggaagaacct ttttgatttc caactaagcg aagttattga ggacgacagt
     4321 aagaccaaaa ccgcattaag taaagtacag gaagcaagac tgctggactt atactctcat
     4381 gcgtcagatg caggaaatgc gaggtctggt agcatgattt acaacgattt gtacaacgac
     4441 tgctagtaga aataatatgg agataagtat tatctacaaa gtcagaaaag aaatgacttg
     4501 ataatttact catgttataa ttattgatgt gaaattaata aatcgaaatt ccttttttgg
     4561 atttaataat actgagtaag gaaaaagtga attacttttg caaggaggtg ttaatatcag
     4621 gcaagtaaag tcttttgttt ttccagttat atccttaata ttcttagacc aaattagcaa
     4681 agttcttata ggattattct taatggactt tgaaattgat ataattggga aatttttaag
     4741 attcaatcct gttcaaaata caaatctctc ttatggggga aactttattg gtattctatc
     4801 taatttatgg gtattggtat tgtttaacat tttagttata ttagttatta tatctggata
     4861 tgctttttat aaatcaaaaa atgaacaaac aagctattca gtaaaagtaa ttatgtcttg
     4921 tggacttgct ggtacaatat gtagcttgat agataaatta ttttggggag gaagtttaga
     4981 ttttttgcag ataccaagct tttttatttt cgacttaaaa gactgctacc ttactgttgc
     5041 agaaataata tttgttgtca taggaatttt gcataataga gaaatatcaa tgaaagaata
     5101 catatatttt tgctatcgtc agtttaaaaa ataaatttag aagataaatt taagtttcaa
     5161 ggtaaactta tttaatcaca gcaaatagtt ggcttactta agcttttggg ctaaactgtg
     5221 agcactaatt aagtctctga tatctaacaa acggagcaca aaaataaaaa ttaaatataa
     5281 agcacagtta attttgatta aaaagttgac tgtcttttct tttgtagaaa tttaagcata
     5341 ggtaataaag aaatgactgc atttaagcat tatgtatata attggaggca ataaaaagcc
     5401 agcaaagccc ataaaataga gctgtgctag caacatgatg aattataaga agataatcaa
     5461 aaacttacgc tttattttaa tttaaaaaaa tgattactgt aaagaaatca ttttttatta
     5521 taatcactga tacatcaaat attctaaata aaatttttat ttatctggaa ttgtatattt
     5581 tttctataat attaactaca gcgtacattg cccaagcaca gatagccaat acaaatactc
     5641 ccatcattac taaatctaat ttaaatactt gagaaccata tactatcaag tagcctattc
     5701 catatcttga aactaaaaat tctccaacta ttacacctac ccatgccata ccaatattaa
     5761 ttttagttaa atttattaaa tttcctgtgt ttgcaggtaa tatgagttta aataatattt
     5821 gagatttagt agctccaaaa ctttttaaca tcttaatttt ttcttcatct atattcataa
     5881 aataattata agctgaaagt atagtaacaa caacagaaat cgttacagct gttacgacga
     5941 ttccttctat tccagcacct acccaaacta taattatagg agctaaagca gtcttaggaa
     6001 gagcatttaa aactaccaaa aatgggtcta atatttttga taatttttct gaccaccaaa
     6061 gagcaattgc taccaaaata cctaatacag tacctataac aagaccaaga acagtttcat
     6121 atacagatat tccaatatgt ttaaataatt gaccactaaa tgcatattga ataaataaat
     6181 tgtaaatatc acttggtttg ctaaatagaa atgtatttat aacatttaag tcagctaaaa
     6241 gttcccagat tacaataaaa ccaattaaaa ttaatagctg gtagaataat acatttcttt
     6301 tttctctttt tacaccttta acatagttta tatatccttc agatctttta ttaagcctca
     6361 tgtgtatcta gctccttcca taatatgtcg aaatactttt gaaaatcagg aactttacgt
     6421 gttttgagag gcgtttttgt tccatccatg ttaagttcaa tatcgtaaat attttttatg
     6481 gtagctggtc ttttagatag tattgcaacc ttatctgata tgcttattgc ttctgctata
     6541 gctaaaaaag tgcaactaat tgcttaaaga aacagaatta tcaacttcag cacccatata
     6601 aacaatatta agatttccta aactgctatc ccaaacgata ctattaataa tagtagataa
     6661 taacaatctc ttatcatcta aactggcatt atcaacttca tcattaaatc tcttcaaatt
     6721 actaacaata atatcaatat taatactgtc actactaata ttgatgttac tagattccaa
     6781 agtattaatc ttatccttta aaatactaat ctcattattt aggctctcaa tctgggaaac
     6841 tatataatca gaagcagtac taccagtatt ttttgcaagc tctataacta aatttttaat
     6901 aagtctttcc ttatctaaaa tttgattttt aagtaaatta gtctcagatt taaaaatatt
     6961 aaaaacatta tcagtcttaa tgttattact attgaatgca cttataatag actcttcttt
     7021 acaattctta attttatcta taactaaact ttcaactaat ggaccattaa gatttttaca
     7081 gttgcaacta tcagacccaa gtgttttttt agtaccacaa atataataat actttaaatc
     7141 cttgtcagtt tttttgcctt tataggtaat tctcatatta gaaccacatt taccacattt
     7201 tataagacca gataaaagag cagtttttcc agttgatatc cttggagttt tatcacgatt
     7261 tttatctaat tgattttgta cttcaagcca tatttcaggt tctatgactc catggtgttt
     7321 tgcaattgct gctattgagt tagatgtatt tttagcatag gtaaggattc cacattttct
     7381 atctggagta cctacaacat ccataccact atttttaagg taatctaaaa tttcatcatt
     7441 agcttttaca tatgctggat ttctaagtat tctagataaa gaactagggt caaatttgcc
     7501 accacgagga ccccttattc cattattata gacatattta tataacttag ataaggacct
     7561 aagttctaaa tatttattgt atattagctt tacaatctcc atttgttcat catttacttg
     7621 aagcttatac atacttcttt cttttagatt ttcatctaag aatgtgattt tttcactgtt
     7681 gaatccatga ggtgtattgc ctccaagcca tctaccacta cgggctaatt catacatatt
     7741 gtcttttata cgttctgcta ttgtttctct ttctaattga gcaaatacac ttgatataaa
     7801 catcatagct cttcccattg gagttgaagt atcaaattgt tctttgatag atataaatga
     7861 tatgcttagt tcatttaatt tttctattaa agtagagaag tcagatacat ttctcgatat
     7921 cctatctaat ctataacaaa ctaggtaact aaatttctta tctttggcat cctgcatcat
     7981 tttcttaaat tgtggtctat ctatattgct accactaaat ccctcatctt catatatcaa
     8041 aaaatctttt acaccaatag actttgcata atcaatacat aattgtattt gattttctat
     8101 agaatcacct tttccagtaa atcttgattt tcttgaataa attgcagctt tcaaaattaa
     8161 aaccccctat aagttaggat atatatttga aactatttat ctacaacctt ttaagtaaac
     8221 ttattagaat gaatttatta attaggtaaa tttatataat tctacttttt ataatatatg
     8281 actatttatt ttatttcttt actattcaag ttctaaatca ataataaatt ttgatattta
     8341 attgtatcag gacaaatctc taatttcata gattattaat ataaaatcat aggttagtgt
     8401 gaaaaagttg aaatttaaat attttaaaag ataatttttt tgaactaata ggtataacaa
     8461 aaatttatag ggggattgca tatggaaatc aatgttatag aaattttccc taaagataaa
     8521 gctaaactta ataaaataga aatggataaa gctagttggt ttgtaaatat aataagtaaa
     8581 aaatatccta aagaagcttt aaatgaagca tttagtactt tagaaaaaga attaaatata
     8641 agtaaagcta atacataagc tttgattatt gattttatat actattggtt ttatacgcta
     8701 tgacttgaca ttatattaat caaatagtat atttttaaat atagcacaat gaagtgttaa
     8761 aagagcaatg gagctccata gataaagtct atggagcttt tttgcgtatt taacttaagg
     8821 aggataggac tttgaataac tttgagatta aaactagaat aaaatttgga gaaggctcac
     8881 taaaagcttt gcaagaatta aaaaacaaaa atgtactaat aataacagac ccttttatgg
     8941 tgaaatcaaa aacaatagat aaaataacat ctaatttaat aaactcaaca tatcaagttt
     9001 ttgaagaaat agtaccagac ccaccaatag aagtagtagt agcaggaata gaagcattta
     9061 aatctataaa tccagacgta ataatagcat taggtggtgg ttctgcaata gatgcagcaa
     9121 aatctataat ggacttttct aaaaaaatat taaaaattaa cgaagtagag tttatagcaa
     9181 taccaacaac aagtggaaca ggttcagaag taacatcatt tgctgtaata acagatagtc
     9241 aaaaaaaatg taagtatcca ttagtgagtg atgatttact tccagatatg gcaatactag
     9301 acccagaact tgtaaaaact gtaccaaatt ttataacagc agatacaggg atggatgtat
     9361 tgacacatgc tatagaagct tacgtatcta caaattctaa tgacttttca gatgcactag
     9421 ctgaaaaagc tataaagtta gtatttaaat acttaataaa agcatataaa gatggaaatg
     9481 acatagaagc tagagaaaaa atgcacaatg catcttgttt agcaggtatg gcatttaacc
     9541 aagcatcact tggcataaat cattctattg cacatgtatt aggtggaaaa ttccatatac
     9601 cacatggaag aacaaactct atattacttc ctcatgtaat agaatataat tcatcaatag
     9661 taggatatac taacacaact tactctgcta cagctaaaaa atatgcacaa atagcagaat
     9721 tagtaggact tgggagtgct aatataagaa tatctgttag aaatttagta aatgaaataa
     9781 agaaactaca aaaagaaatg aatatgccaa ctaaattgac acaatgcaaa ttaagtttag
     9841 aagatataac taatgaagaa agagaaatag caaaacttgc tataaaagat acttgtacat
     9901 taactaatcc taaggtacca actgaagatg atataataaa catattaaag aacattaaat
     9961 aactacattt tattacatgt aaatataata ttatacaaat agggaaaatg tttttaaaaa
    10021 tataaattat atattgacaa atataaaaca taaaactata atgaaagtat cacaaaggcg
    10081 tgatacttta gtagaaagca atggagcttc ttaggaatat taatattatt cttatggagc
    10141 tccttttatt ttaatcaaat agctttttaa taaactatat tcaaagaaag cagggaataa
    10201 atttgactga agaatctaag caaagagtta ttcaagaata tgttcctgga aaacaggtta
    10261 cattagcaca tatcatagct aaccctaatg aagatatata taagaagtta ggattagtac
    10321 ttgataaaaa agatgcaata ggaatactta caataacacc aagtgaagcg tcaataatag
    10381 cagcagatgt tgctacaaag gcttcaaatg tatctttagg ttttatagat agatttagtg
    10441 gttcagtagt aatatcaggt gatgtaagtt ctgtagaatc agcgttaaat gatgttttag
    10501 aagtcttagg aaatatgtta aacttctcat ctacaaaaat cacaaggaca taataataca
    10561 atgaagaata taatatttat gggaaagaca ggtagtggta aaactacctt atgtcaaaaa
    10621 ctagaccagt tagaattaaa gtataaaaaa actcaatcag tggaacttta taataatgca
    10681 atagacactc ctggtgagta tatggaaaat agaaacctat ataatgcctt aataacaaca
    10741 tcagcagatg ctaaaattat agcaatagtg tatgatgcca cacaagaaga aaattatata
    10801 gcaccaggtt ttgccagtat gttttgcaaa gacgtaatag gaattataac taaaataaat
    10861 aaagctactg aaaacgaagt caatatagct tatgaaagac tatcactagc aggagcaagt
    10921 caaatcttta aaatagacac agttgataat attggacttg aaaaattatt taaatatcta
    10981 gaaaaatata aaaatataga tgaagtttaa ggggaaatat ttttttattc aaaaatatta
    11041 ttaaagtatg ataaaataaa aaaatgtata aactatttta agaagattta atctttaagc
    11101 gaggtaggaa catggggaaa aaaatactat tggttgatga tgaaccactg ttaaggatgg
    11161 atacaaaaga tatattagaa agtcatggct atgaaataat aggcgaagca agtgatggat
    11221 ttgaagctgt tgaaatgtgt aaaaaacaca aacctgattt agttataatg gatatagaga
    11281 tgccaatact tgatggtata aaggcaggca aaataatatg taaagaaaat atagctggtg
    11341 gagtactttt attaacttca tttgaagata aagaatacgt agaaaaggct aaaagtatag
    11401 gtgcttatgg atatttagta aaaacagcaa gtgaaaaatc acttatccca accatagaaa
    11461 tgtgtttaaa taaggtaaat gaatttgaaa aaatgaaaaa agatttagaa aaagtaaatt
    11521 ctaaattgaa tgaaagaaaa ttagtagaaa gagcaaaagg tatacttata agagagttta
    11581 agatttctga ggatgaagca tacactagaa taagaaaatt aagtatggat aaaagaacta
    11641 caatgatgga aattgccaaa atgattataa taggatacga tgactaatac gataaaaaaa
    11701 atatcaactg cacaaacata cttaaataat aacgaaattg aaaaaattat agaagtatca
    11761 aaatccttag atttaatggc ttctttttat gaagctgatg tttttataga tgtactaagt
    11821 aaatttgatg atgaagcaat tgttgtggca catggtagac ctaaagaaaa gtcaatgtac
    11881 tgtgaaaatg ttataggtaa aaaggcttta attgaaaatg aaccaggagt tataaagtgc
    11941 ttaaaaacag gagaaagagt aagagatatt aaagctttaa cacaggaata taaactggtc
    12001 aaacaaaata ttcagccaat aacactagat gacaaagttt tagcagtact aatagttgaa
    12061 aaagatataa gtagtgaagt taagaatgag tttaatgcta gtgaagacga agaaaataaa
    12121 aatttacatc agtttataaa acttattgga aataatgact ttttagttga taatttaaat
    12181 tcagccatat taatatttga taaaaatgga aaattaaaaa ttaaaaatcg tattgcaact
    12241 gagatataca aaaatcttgg gtttggatat gatatacaag atatgcatta caatgattta
    12301 gatatagaaa ccaacacctt tgaaaatata ttaatggatg aaaattatga tgagacaaat
    12361 gaagtttcaa taggtaatat gttttttaaa ataaagacca tatacatcaa agatgatgaa
    12421 tttaaggttt gcaaaatagt acaagatata agtgatttaa agagaaaaga agaagaatta
    12481 gttttgaaaa cagtggctat aagagaagct catcatagag taaaaaataa tcttcataca
    12541 gtaatatcta taataaaaaa acaaagtaga ttatctcaaa atgatgaagt aaagcagtgt
    12601 ttagataaca taataaatag ggtatttgca atactttcga ctcattattt actatctaaa
    12661 gaagttgata ataatatatc tatacgagaa ggtatcaatt tattagtgtc aaatattcaa
    12721 ggtggctatt tatttagtaa ggatatagat atacgtataa ctggtgaaga ttttaaaatt
    12781 agtggagaaa aagcaacagc aatacttttg gttataaatg aaatactaca aaattgttat
    12841 gaccatgcat tttcggatag agaacatggt actatacaaa tattaataaa taaagataac
    12901 gactatagaa atattgctat tatagatgat ggaattggat ttgatagtaa taatataaat
    12961 aaaaatagct tagggacata tataatagat agttatataa cacagatgtt aaaaggaaat
    13021 atagagagaa aaagtagtga gaatggtaca gaagtattaa ttactatacc tacacaaatt
    13081 aatatttaaa taataactca tctacaaagt agtagtttta attgagcaaa gaggcttaag
    13141 gttattaata gattaagtta attgaatgta tttataaaaa ttataagtat atattcaatt
    13201 taacaggtat ttattataac tttaagcctc tttgcttgtt taagacatac ttaaatttta
    13261 ggaggtgaga aaatgagaga agagttatta agtgttggta ttgacattgg aacaagtact
    13321 actcagttaa ttttttctaa attaatagta gaaaatactg aatcaaattt ttcggtacct
    13381 agaataagta tagtagacaa agaaattatt tataaaagca aagtttattt cacccctctt
    13441 atttcaaata atgaaataaa tggtcaacaa ataaaagata tagttgaaaa tgaatataaa
    13501 aaagcaaata tatctaaaga agatatacat acaggagcag taataataac tggtgaaaca
    13561 gctagaaaag aaaatgcaaa tgatgtactt catactttga gtggatttgc tggggacttc
    13621 gttgttgcaa cagcaggacc tgatttagaa agtataatat caggaaaagg agctggagct
    13681 catatttatt caaaagaaca ttcaactaca attgtaaata tagacatagg tgggggaact
    13741 agtaacatag cctcatttaa aaggggagaa gtaaccgata ctgggtgttt ggatataggt
    13801 ggaaggcttg taaagataga taaaataagt aaaaaaataa cttatatatc acctaaaata
    13861 gataaaatca taaaagataa taatttaaat cttcaagtag gcacaatggc tactgtagaa
    13921 aatacaaagc caatagtgga tataatggtt gacttattaa tggaaagcat aggattaaag
    13981 aaacagacac ctttgtttga ttttataata accaataaat caataagtat agatgaaaat
    14041 attaaaaatg tttcattctc tggtggagta gctgattata tttactatga tggagaaata
    14101 aaagattatt ttgaatatgg tgacttggga attttacttg ggcaagcaat aaaaaattgt
    14161 gaatacctta aaaaagtaaa tgtagttaaa tctatggaaa caataagagc cacagtggtt
    14221 ggagcaggtt ctcatactac tgaagtaagt ggaagtacaa taacttacac tagcgaaaag
    14281 tttccactta agaacttgcc agttttaaaa ttatcaattg atgaagaaac aggcacttca
    14341 aaacaaatca gtgaagctat aaaaaataaa cttaattggt ttaaactaga aaatgattta
    14401 cagaaagttg ctatagccat gataggaaag aaaaatccaa cctttttaga tatacaagag
    14461 tatgctaaag ggtttgtaga tggaatgcaa caaatcatag aaaatgattt accttttata
    14521 gtaattgttg aaaatgatat ggcaaaagtg ttaggtcaag caatatatgc attattaaat
    14581 tatgaaaaag aaattgtttg tatagacagt gtcaaggttg aaaatggaga ctatatcgac
    14641 attggaaaac ctatagttga aggtaaagtt ttacctgtgg taataaaaac attagttttt
    14701 aattaataga aggaggtaag taaatgattt taaatacaaa attatttgga cgtacttatc
    14761 agtttaaagg cttatatgat gtaatggcta aagctaatga agaaaaatca ggagataaat
    14821 tagcaggtct tgcagcagaa tcagctgaag aaagaatagc agcaaaagtt gttttatcac
    14881 acataaaatt ggaggatata agaaataatc ctgcagtggc atatgaagaa gatgaagtaa
    14941 caagaataat acaagatgct gtaaatgaaa aagtttatga agaaataaaa aattggacag
    15001 tctctgaact tagagaatgg ctattggatt caaatactac aagtcttgat ataagaagaa
    15061 tatcaagagg attaacttct gaaatgatag cagcagttgc aaaattaatg tcaaatatgg
    15121 acttagttta tgcagctaaa aagataaagg ttactgctca ctgtaataca actataggag
    15181 aacaaggaac attatcagca agacttcaac ctaatcatcc aacagacgac ccagatggaa
    15241 tacttgcatc acttcttgaa ggtttaactt ttggtatagg agatgcagta cttggattaa
    15301 atccagttga tgactctgtt gagagtgtta ctaaagtatt aaaaagattt gaagaagtaa
    15361 aacaaaaata tcaaatacca actcaaacat gtgtattggc acacgttact acacaaatgg
    15421 aagcaataag aaatggtgct cctgcagatt taatattcca atctatagca ggttctgaaa
    15481 aaggaaatga agcatttgga tttaatggcg acactataga agaagctaga cagttagcct
    15541 taaaacacgg tacagctaca ggacctaacg ttatgtattt tgaaactgga caaggttcag
    15601 aattatcttc agaagcacat catggaatag accaagttac tatggaagct agatgctatg
    15661 gatttgctaa aaagtatgaa ccattcttag taaatacagt tgttgggttt ataggacctg
    15721 agtacttata tgatgctaga gaagttgtaa gagcaggact tgaagaccac tttatgggta
    15781 aactacatgg cttaccaatg ggtgttgatg tttgttacac taaccatatg aaggctgacc
    15841 aaaatgatat ggaaaactta gccatactat taacaacagc aggatgtacg tattttatgg
    15901 gaatacctca tggagatgat gttatgctta actatcaaac aacaggatac catgaaacag
    15961 catcacttag agaaatgttt ggactaacag caataaaaga atttgatgct tggatggaaa
    16021 agatgggatt ctctaaagat ggtaagttaa cagagttggc gggagacgca tctgtattat
    16081 taggatagga ggtatttaat atgaatgaga aggatttaaa agcattggta gaacaattag
    16141 ttggacaaat ggttggagaa ttagatacta atgttgtgtc tgagactgtt aaaaaggcaa
    16201 ctgaagttgt agtagacaac aatgcttgta tagatgatat aacagaagtt gatataagaa
    16261 agcaattact agtaaaaaat cctaaggatg cagaagctta tttagatatg aaagcaaaaa
    16321 cacctgcaag attaggtata ggaagagctg gtactagata taaaacagaa acagttttaa
    16381 gatttagagc agaccatgct gctgcacaag atgctgtatt ctcttatgta gatgaagaat
    16441 ttataaaaga aaataatatg tttgcagtgg aaactttatg taaagataaa gatgaatact
    16501 taacaagacc agatttggga agaaaatttt ctccagaaac tataaataat ataaaatcta
    16561 aatttggtac aaatcaaaag gttttaatat tagttggaga tgggcttagc tcagctgcaa
    16621 tagaggcaaa tttaaaagac tgtgtgccag ctataaaaca ggggctaaaa atgtatggta
    16681 tagattcaag tgaaatttta tttgttaaac attgtagagt tggtgctatg gaccacttag
    16741 gtgaagaatt aggttgtgaa gttatatgta tgttggttgg agaaagacca ggattagtta
    16801 ctgctgaatc aatgtcagca tatatagctt ataagccata tataggaatg gcagaagcaa
    16861 aaagaacagt tatatcaaat attcacaaag gtggaacaac tgcagttgaa gcaggtgctc
    16921 atatagctga acttataaaa actatgttag ataaaaaagc ttcaggaata gaccttaaat
    16981 aaagtcgagg gagtgtagaa tatgaaaaat gatgtaattc gtcctaatat actaggaatt
    17041 aaaataattt caaatataag tccagaaatg gcacaaaaac tagaattaaa atcacatcac
    17101 aaaagcttag gtttaataac tgcagactgt gatgatgtta cgtatacagc tcttgacgaa
    17161 gcaactaaag cttcagaagt tgatgttgta tatgcaaaat caatgtatgc aggtgctgca
    17221 aatgcttcaa caaaacttgc tggtgaagta ttaggaataa tagcaggtcc aagcccagca
    17281 gaagtaagaa gtggtctaaa tgcagtagtt gattttctag agtatggagc aacttttata
    17341 agtgcaaatg atgatgattc aatagcatac tatgctcact gcgtatcaag aacaggtaca
    17401 tacctttcag aggtagcagg aataagagaa ggtgaagcat tagcttattt agtagcacca
    17461 ccacttgaag caatgtatgc actagatgca gcaatgaaag ctgcagatgt taagatgtgt
    17521 gaattatttg cacctccaac tgaaacaaac tttggtggag cattacttac aggttctcaa
    17581 tcggcttgta aagcagcatg tgatgcattt gcagaagcag taaaatcagt tgcagacaat
    17641 ccaacaggat tttaaggata gttagatgga tgctataggc ttaatagaag taataggata
    17701 tgttgctgca atagaagctt ctgatgcttg tttaaaatct gcaaatgtta atattgtaag
    17761 agttgataag gttggagcag gtattgttac tttaacaata tgtggagatg tcggtgctgt
    17821 aaaagctgct ttagaagcag gtgaaatagc agcttccaga gttggtacat taagaacatc
    17881 acatattata ccgagaatcc ataatgaagt aacaaatgct ttattcaaaa ctaaagaacc
    17941 taaagtctgt gaagtttcag aagataaaga aaaaaacttt gagattacat tggaaatagc
    18001 tgataatgta tcgaaaaatt tatcagaaaa agaaaatgta attttagatg tcaatgaaaa
    18061 cagtggagat actgaaaatt tatatagtga tatagataaa acaaacaaaa aaattgaaac
    18121 agttgatgat agtttaacag aagctaatga ggaaaaaata gaaaacacta atgataaaga
    18181 agtattggaa caagttaaaa tagaaaattc cgattttgat agagatgacc tatcaaagaa
    18241 cagtgtaaaa aaacttaaag ctatggctaa gaaattagat tcttcaataa cttataaaga
    18301 cttaaatgtt cttaaaaaag aagaattaat aaacttagtt aaaaagctta gtagaaggga
    18361 tagatagtta tgacactttt agataaagat ttagtatcta tacaagaagt aagaactctt
    18421 gtaagaagat ctaaagtggc tcaagagaaa ctttctaaaa tgagtcaaga gcaaatagat
    18481 aaaatagtaa aagctgttgt agatgcagca tttaataatg ctactaaatt agctaaaatg
    18541 gcaaatgaag aaactggatt tggaaaatgg gaagataaag taattaaaaa tacttttgca
    18601 tctatgggag tttatgaaac tttaaagaac acaaaaacta ttggaataat tggtgaagat
    18661 aaagaagcca atacgataga tatagctaca ccagttggag ttgttgcagg tcttatacct
    18721 tcaacaaacc caacatcaac agtaatatac aaaactataa tatctctaaa agcaggaaat
    18781 actatagttt tctctccaca tccaaatgca aaaaactgca tacttgaaac agttaatata
    18841 ataagtgatg ctgctataaa ggctggagct ccagaggatt caatattatg tataactaat
    18901 ccaagtatag ttggaaccaa tgaattgatg aaacataaag atacaaactt aatactagca
    18961 actggaggag aagcaatggt tagagctgct tactcttcag gaacaccagc tattggagtt
    19021 ggaccaggaa atggaccagc atttatagat aaatcagcaa atgttaaatt agcaatcaaa
    19081 agaataatag attctaaaac ttttgataat ggtgttatat gtgcatcaga acaatctata
    19141 gtaatagaaa aatctatgga agaaactgtt ttaaaagagc ttaaaaatca aggtgcatat
    19201 gttttaaatg aggaggaaag tgaaagatta gcaaaattca tattaagagc taatggtact
    19261 atgaatccac aaatagttgg taaaagtgtt gaggtaatag caaatttagc agacttaaca
    19321 aatgtaccta aaaatacaaa agttctagtt gcaagagaaa ataaagttgg tcacaatgta
    19381 gcctattcaa gagaaaaact tacaccaata ctagcgttct atgttgaaga aaatgtagaa
    19441 gctattatga acagatgcag agaaatattg ttaaatgaag gtgcaggtca tacattctca
    19501 atgcatgtaa atgatgaaga attggtaaaa agatttgccc ttaatatgcc agtatcaaga
    19561 atacttgtga atactccttc agcacttgga ggaataggtg catcaacaaa ccttgtacca
    19621 gcactaacac ttggttgtgg tgcaataggt ggaagttcaa cttcaaacaa tataggacca
    19681 cttgatttaa taaatgttaa aagagtagct tatggaatca aagaaataga agaacttgga
    19741 agagataaat ctcaagttga aaaatcaatg tttgactcaa tggataaaga agaattaata
    19801 aatcttcttg ttcaaaaact agtcaaagaa ctagcataac taaaataaac aatgaaactt
    19861 atggctatcg ctataaattt ctaaaaatat aaactataaa aaactataaa attaaatttt
    19921 tggaggataa gattatggca agtgcaaacg cattaggaat gatagaaact aaaggattag
    19981 taggagcaat agaagcagca gatgcaatgg ttaaagctgc aaatgtacaa ttagtaggaa
    20041 aagagcaagt aggtggagga ttagttactg taatggtaag aggagatgtt ggagctgtta
    20101 aagctgctac agatgcagga gctgccgctg ccgagagagt tggagaacta atatcagttc
    20161 atgttatacc aagaccacac tttgaagtag atgctatatt accaaaggta tcagctgagt
    20221 aatataagaa taattataaa atttaaattt tgattgaaaa agaggctatc tcaaaaatga
    20281 gtcttactaa taaactcaat gtattttgag gtagtctttt ttatacatct aataggagat
    20341 gtgataaaat gggtgtttta acagaaaacg aagtaaaaag aaaaattgga aaaaacaaaa
    20401 ataatataac tgaattttat atagaaaaag gtcaaataat aactccttct gcaaaatctt
    20461 atttagctga gagaagtatt gagcttaaat atgttgataa actttcagat gtaaacaatg
    20521 aacctaaaga agttgtaaaa gaagtagtta aagagaaaaa atatagatat actactgtat
    20581 ttggcgctaa attggatgaa aaaccagaac atatgactca attaaatgga aatcttttag
    20641 tttttaaaga ccataaaaga ataatattga gaggaaaaat agattctctt gaatcagaaa
    20701 tactaaaggt acagatactt tgtcagaaac tagatatgcc aaagcttgtt aaggaattgc
    20761 aagaagtatt agactttgta agaaatataa tgagatgtga agttttagat gaaaagctag
    20821 gggactttaa acttttagga ttaactcctc aagaacttag agaacaatct cactatccta
    20881 aaaaatactt tggtatagga catgagttcc caagttatga aatgggagaa gtagtagtag
    20941 ctataaatag tataagaagt aatacacgag aggttgaact tgcagcctat gaagcattta
    21001 agggtgagta tggaaatgtt cacagagaag atttaataaa ggcacttaat agattatcgt
    21061 cagtattttg gataatgata tataaggtta gaactaataa gtataagtag ttggaggaca
    21121 taatggagaa tctattaaat gaaatagtgg aagaagtaat aactagagtt aaaaaagaag
    21181 catttataga agttgaagca tcaggaagac acgttcactt aagtagagaa gatgtcgata
    21241 aattatttgg agaaggatat actcttacaa aactaaaaga cctatctcaa ccaggtcaat
    21301 atgcatgtaa ggaaagagtt acaataactg gtcctaaagg aagtataaaa aatgtagttg
    21361 tacttggacc atgtagaaat gaaactcagg tagaaatatc tttaacagat ggttcaactc
    21421 ttggactaaa agctcctata aaacaaagtg gagacttaga aggtacttta agtattaaaa
    21481 tatcaacaca atatggtgaa gttgaacttg ataaggggtt aatggttgct aaaagacata
    21541 tacacatgac tcctaaagat gctgaaaaat tcaatgtatg tgataaagag atagttgaag
    21601 ctaaggtgat gggtcaaaga cctttaacat ttgatgatgt agtaataaga gtgagtgaca
    21661 gttttaaaac ttatatgcat atagactatg atgaagccaa tgcatgtggt tattcaaaag
    21721 gaactgttgc taaaataata aagaaagcgt aaatttaacc tactatataa attacctcta
    21781 gggaggattt aatatgaatt atgataacct tgtagattta ataacggaag aaatctataa
    21841 aaaaataaat agtaatgaaa taaaaatatc taataaacct aaagctgtga ttgtatttga
    21901 acaagataac aataaattta acttattaaa agatgaattt gaaatagtag aatttgataa
    21961 aagtataagg gaatgtgaaa tagtgatagt ttcaagactt tgtatgaggg ggctatcgaa
    22021 tatagcactt ggaaattcaa caagtgatga agaaagattt atactaaaaa tgattatgaa
    22081 gggcaaagaa gtatatgtcc tagatgaagg aattgaatac aaaaaatata aggatactgc
    22141 accaaaagca ttatataaga aatttatgtc atttgaagat gaaatatgta aatttggtgt
    22201 agaaattata aaagacttaa atgttataac taaaaataag ttaaatataa agaaagaact
    22261 agaaagtact agaggttcta aaaaagaaga tacaaaagtt ttagataaag atgttttaaa
    22321 tttaaccagt gtagaaagtg taaaaataga taatgaattt agtttagatt taaggaataa
    22381 aaaacttata tcggaagctg acttaagaaa accaactata aatggagtta aaaacatatt
    22441 agttaataaa aaaagtataa taacaccatt agctgtagat tttatgagaa tacaccatct
    22501 caaactaaag aaagtgtaag gaggtaattt tatggatata ggaaaagttg taggtaatgt
    22561 ttgggctaca agaaaagatg aaaaattatg tggccaaaaa cttttagttg taaaaatttt
    22621 aaaaactaaa gacacataca aggaaggctt catggttgca gccgataatg caggagcagg
    22681 aaatggagac ttagttctta tatccaaagg ttcctcagct agagagtcga ttgaaaaatc
    22741 tcatgttccg gtagatgcaa ctatagttgg agtagtcgat tcgtttgagg tgtttgatga
    22801 ataaaagtat aggagctata gagtttaaaa gcattgctaa aggtatagaa gtatccaatg
    22861 aaatgataaa gaaatcttca gttgatgtat tgtacttaaa aagtatttgc ccaggtaaat
    22921 ttctaataat agtaggtgga gaaacttctt atataaatga atgtgtagat tatggaataa
    22981 aacttggaga aggatatatt gtagataact tcgtaataaa tgcaatttca caagaaatat
    23041 tagatggatt taaaaataag tatcaaaagc tagatagtat agtctctata ggcgttgttg
    23101 aaagtagtaa agtatgcgct ggaataaaaa tgcttgataa gactttaaaa tctggcgact
    23161 tagtattagt aaaacttcaa ttatcttttg ctataggtgg caaattagta tatatagtag
    23221 caggagattt aagtagttta gaatatgcat taaaagaaag tgaaaatgta gtaagagaaa
    23281 aagaagtaat atataagact gtaataccat cagtagacag tcaaataata aaaagtctaa
    23341 taaaataatg gggggaatta tgagtataaa cgaaattata atctatgtaa tggtagtctt
    23401 catggtactt ggagctatag ataaatgtat aggtaacaaa tttggtcttg gagaacaatt
    23461 tgaagaaggt ataatggcaa tggggtcact tgcagttgca atggttggtg ttatatgttt
    23521 agcaccagtt cttgctgatg tattaagacc aattgtagta ccagtattta atatacttgg
    23581 agctgaccca gctatgtttg ctggaagttt attagctaat gatatgggtg gtgcaccact
    23641 tgcacttgaa cttgcgaaag accctaatgc tgggttattt ggtggattaa tagtaggggc
    23701 tatgatggga cctacaatag tatttataat accagttgcg cttggaataa ttgaaaaaga
    23761 agaccaaaaa ttcttagcaa caggaatact tgcaggtata ataacaatac ctattggagc
    23821 attcgttggt ggattagttg caggattcga ggtaatgatg gttcttagaa acttagtgcc
    23881 aataataata tttgcagtag ttatagcact tggattatta aaattcgaaa atgctatgat
    23941 aaaaggattc acatactttg gaaaaggtgt agtaatagtt ataactttag gacttgctgc
    24001 tgccatagtt gaagctttaa caggaatagt tgtaatacct ggaatgaaac caatcagtga
    24061 aggtatagaa atagttggag atatagcaat agttttagca ggagcattcc cattagtgtt
    24121 tgttataaca aaggtattta ataaaccatt aatggcttta ggaaaagttc ttggaatgaa
    24181 tgatgtatca gcagcaggcc ttgttgcaag tttagctaac tgtataccaa tgtttggtat
    24241 gatgaaagat atggacaata gaggaaaaat aataaatgta gcattctctg tatcagcagc
    24301 attcgtattt ggagaccact taggatttac ggcaggattt aattctaata tgataacacc
    24361 aatgatagtt gctaaattag ttggtggtat aacagcagta ttgttagcta tggttatcgc
    24421 aaataagaca ctaaagaagg agaatgtata agatggatat atcaaatata gataaaaatt
    24481 taattgaaac attagtaaga caaataatag aagaaaaaat aagtggaact aaagacacag
    24541 tagattttgt tagaaataaa gacattagtg gaataacttc tataaagtta ccaactgtaa
    24601 aagttagtga atctgataga ttagatacag ggaatccaag tgatgtagtt tacacaaaag
    24661 atttatttac attagaggaa agcccaagat taggttgtgg tatgatggaa atgaaagaaa
    24721 caacttttga ttggactcta aactatgatg aaatagatta tgtaatagat ggaactttag
    24781 atataataat agatggaaga aaggtttctg cttcttcagg tgaattaata tttataccaa
    24841 aaggaagtaa gatacaattt tctgtacctg attatgctag atttatatat gtaacatatc
    24901 cggcagattg ggcatctcaa aattaataaa aatcaagtta agttaataat aatcaaataa
    24961 ataatttaaa atacaaactc ttaaataagc acacatattt aaaaaagcta tgaattaaaa
    25021 ttcatagctt tttttataaa gaatgttata ttttagacgt attttttcta tatttactgg
    25081 gagacatatt atatttttta ttaaataatc taataaaatg ctctacattt ggataaccaa
    25141 tactatttta ctgataatct tttccttatc actaatctta cattttaaaa tattattttt
    25201 actcgtaata ttatcataag aatttgtttc ttatttaaaa ctaattagaa ttataaagtt
    25261 tattgccatg ttattaataa aataaatcca gttaaatcga gtgctagtta aagtaataag
    25321 aaacttttac aatggtttcc tttatcgatt gaaataaaag ttaatatggt ataatatcta
    25381 aagagagaat cttatcaatt agaatatgca tagaaacaag tggcttaaaa ggagacgtta
    25441 tatggaagag atagtaaaga aggaacaagc aagtatgaat gatatttttt tgtatcttaa
    25501 agctaaatca aactcattat ataaatttgt aatattatat aatgagttta tcaataaacc
    25561 taaagattat ggaactgggg aacttataaa catggttgaa gtacatatgc taacagcgat
    25621 tgaagaaaat ccaggaataa gtccaactca gttagctaaa atatggaaca gaactaaagg
    25681 tgcaatatct caaactatat ctaaattgga gaaaaagggt tatataacta aagaaaagaa
    25741 agaaggaaat gcaaaaacta tattactata tccaaccaca tcaggagtag agttaagtaa
    25801 ggcacataaa ctatatgaca ccatagatgt tacaagaaca tttgaatact taatcaagga
    25861 gtgtacatta gaggagatag atagttttta taaagtaatt gatttgttta ttaatttatt
    25921 agaagaatct ccgcaggaaa ataatgattc tgaaaaagtg tagctgagat tgaagttgaa
    25981 taaagagaaa tagatattat agaagtagta cttaagtaca cattatataa aaatataata
    26041 ggtataggat taaaaagttt agtggtttaa agtttaacca ctaaactttt
//
LOCUS       DAYAOX010000033        25460 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.033, whole genome shotgun sequence.
ACCESSION   DAYAOX010000033 DAYAOX010000000
VERSION     DAYAOX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.033"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            84..626
                     /locus_tag="ACGI57_001111"
     CDS             84..626
                     /locus_tag="ACGI57_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9716196.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            631..3132
                     /locus_tag="ACGI57_001112"
     CDS             631..3132
                     /locus_tag="ACGI57_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9716197.1"
                     /translation="MFTLNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPAR
                     TIIQNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLI
                     CGLSSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIA
                     NILGSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGT
                     LMLICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILS
                     FEYFLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIA
                     APLSGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFT
                     MGTPLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDL
                     VGKPSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKS
                     LLNKVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGE
                     NKLNSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKE
                     LTTAYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVN
                     EQKESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRTMESQKKELQSKLNNSISQ
                     KKDMEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTL
                     QSTLNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            3553..3951
                     /locus_tag="ACGI57_001113"
     CDS             3553..3951
                     /locus_tag="ACGI57_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9716198.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(4168..4377)
                     /locus_tag="ACGI57_001114"
     CDS             complement(4168..4377)
                     /locus_tag="ACGI57_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716199.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(4409..4618)
                     /locus_tag="ACGI57_001115"
     CDS             complement(4409..4618)
                     /locus_tag="ACGI57_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFG9716200.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            4917..5336
                     /locus_tag="ACGI57_001116"
     CDS             4917..5336
                     /locus_tag="ACGI57_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9716201.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            5564..6019
                     /locus_tag="ACGI57_001117"
     CDS             5564..6019
                     /locus_tag="ACGI57_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716202.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(6240..6500)
                     /locus_tag="ACGI57_001118"
     CDS             complement(6240..6500)
                     /locus_tag="ACGI57_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716203.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(6763..7116)
                     /locus_tag="ACGI57_001119"
     CDS             complement(6763..7116)
                     /locus_tag="ACGI57_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1375 family protein"
                     /protein_id="HFG9716204.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(7133..12454)
                     /locus_tag="ACGI57_001120"
     CDS             complement(7133..12454)
                     /locus_tag="ACGI57_001120"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716205.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFIKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGNGNTMLIDNKGILKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            complement(12471..13454)
                     /locus_tag="ACGI57_001121"
     CDS             complement(12471..13454)
                     /locus_tag="ACGI57_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9716206.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKVD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(13455..14153)
                     /locus_tag="ACGI57_001122"
     CDS             complement(13455..14153)
                     /locus_tag="ACGI57_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFG9716207.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(14150..15202)
                     /locus_tag="ACGI57_001123"
     CDS             complement(14150..15202)
                     /locus_tag="ACGI57_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFG9716208.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(15195..15623)
                     /locus_tag="ACGI57_001124"
     CDS             complement(15195..15623)
                     /locus_tag="ACGI57_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFG9716209.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(15623..15949)
                     /locus_tag="ACGI57_001125"
     CDS             complement(15623..15949)
                     /locus_tag="ACGI57_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HFG9716210.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(15965..17494)
                     /locus_tag="ACGI57_001126"
     CDS             complement(15965..17494)
                     /locus_tag="ACGI57_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9716211.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLNVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(17776..18198)
                     /locus_tag="ACGI57_001127"
     CDS             complement(17776..18198)
                     /locus_tag="ACGI57_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFG9716212.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(18198..20651)
                     /locus_tag="ACGI57_001128"
     CDS             complement(18198..20651)
                     /locus_tag="ACGI57_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716213.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MILAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     VWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(20651..20818)
                     /locus_tag="ACGI57_001129"
     CDS             complement(20651..20818)
                     /locus_tag="ACGI57_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716214.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(20845..21291)
                     /locus_tag="ACGI57_001130"
     CDS             complement(20845..21291)
                     /locus_tag="ACGI57_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9716215.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(21417..21845)
                     /locus_tag="ACGI57_001131"
     CDS             complement(21417..21845)
                     /locus_tag="ACGI57_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFG9716216.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYIGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(21859..22923)
                     /locus_tag="ACGI57_001132"
     CDS             complement(21859..22923)
                     /locus_tag="ACGI57_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFG9716217.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(22928..23371)
                     /locus_tag="ACGI57_001133"
     CDS             complement(22928..23371)
                     /locus_tag="ACGI57_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFG9716218.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(23620..24060)
                     /locus_tag="ACGI57_001134"
     CDS             complement(23620..24060)
                     /locus_tag="ACGI57_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716219.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(24165..24362)
                     /locus_tag="ACGI57_001135"
     CDS             complement(24165..24362)
                     /locus_tag="ACGI57_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9716220.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            24531..24851
                     /locus_tag="ACGI57_001136"
     CDS             24531..24851
                     /locus_tag="ACGI57_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9716221.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            24933..25439
                     /locus_tag="ACGI57_001137"
     CDS             24933..25439
                     /locus_tag="ACGI57_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFG9716222.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
BASE COUNT         8380 a         4225 c         2838 g        10017 t
ORIGIN      
        1 ttatagctta catattgatt aataaatttt aaaggagtat aataaacagt gttcataata
       61 attttaataa aagaggtggt aatatgccaa agattttaga aaatgtaaaa gaagatatat
      121 taaaagtgtc tcgggatatg attttagaag aagattactc gaatattagc ataaggaaaa
      181 tagctcaaag gtgtggtata agtgcaggta cagtgtataa ttactttaat tcaaaacaag
      241 aaataataga atatatcaca aaatctgaat gggatttatt gataaggcga attgaatact
      301 ccaataaaaa cactgaagat tacataaaaa aactaaacat tatttttact gaaataagaa
      361 attttataaa caaagttcat aatatccaat acaatgactt tttaaacact tttgaaacag
      421 agagattttt tgaaatgaaa aaacacaaag acgactttca tgaacagctt tcaaataaag
      481 tatatgaagc tttagaaaaa gaaagttttt ttaataatga caaattagta tgcaatatca
      541 taataaaaat gtttttttca tattcaacat atcttcatat agaatttgaa gatttaaaac
      601 cctatattga aaaattaata cattaaggtg gtgtttacct tgaatcataa tactagtatt
      661 aagaacaagg acagtaatcc aaaaatattt ttagtactta cgctttatct attaggtata
      721 tttatgggag ctattgatac tggaatagta tcaccagcaa gaacgattat acaaaatagc
      781 cttggagtaa atgaaaaaac gggaatttgg atgattacca tatacacatt aacatacgct
      841 tctgtaattc ctatctctgg aaaattagca gataaaatag gaagaaaata tgtgtatcta
      901 gttagtatat ttctatttgg tagtggttca ttaatatgtg gattatcatc actattttcc
      961 aatttctata ttttattaat tggtcgtgtt attcaagcaa ttggtggtgg tggaatcatg
     1021 ccaattgcta cagcagaatt tggaactacc ttcccagaaa ataaaagagg catggcttta
     1081 ggtttagttg gggcaacata tggtattgcc aatattttag gttcaagcat tggtagcaca
     1141 attctaagta tctttggaac tcaaaattgg aagtggctat tttttgtaaa tcttccaatt
     1201 tgtttaatca tacttattgg tggagttttc tgtattaaaa acaataagtc tgaatctaaa
     1261 gaaaaaatag ataagcttgg tacattgatg ttgatatgta taatcgtatc actattatat
     1321 ggtcttatga atatagactt ttttaatttt aaaaattcta tacaagatat atcagtttat
     1381 ccatacttat tgacatttat tgtacttata cctattttca tatttattga aaatagagct
     1441 aaagacccta ttttaagctt tgaatatttt ttaaatccga gaatattgat agtacttatt
     1501 ttatctttaa ttgttggtat tggaatgatg ggtatggtat tcgttccaca gtatgctgaa
     1561 aatgcattaa agataaatgc tggttctggg ggatattttg tagctatatt gggattattt
     1621 gcaggtattg ctgctccttt aagcggaaga cttattgaca agtttggggc aaaaaaaata
     1681 ctattactag gattttctgt atctatgata ggctctcttt acctagtttt aattgctcta
     1741 aaaaccaata ctgtatttag tgtatgtgta agtttaatat ttatgggatt aggaatggga
     1801 tttactatgg gaactcctct aaactatatg atgttatcaa atacaagatt agaagaatct
     1861 aattcagcac ttgcaacatt atccttgata cgctcaattg gtacttcaat ttctccagct
     1921 ataatgattg gatttatagc acatgcagga ttatctgtgc aagataatat tatggactta
     1981 gttggaaagc cctcaactcc taaaatagtt caacttgaag aacttgaaaa catgatagat
     2041 gacttaaaat cagaccctga aatggcaaaa aatttaaaaa atgtctctat cccaaatatg
     2101 aatgatactt caaatataaa tatggacatg acaggtggca aacttccaaa atccttactt
     2161 aataaagttc agtcagcaga tacaaccaac attaccaata taacaaaaga aatatcaaca
     2221 acaatgtttg atgaaaaagt tccatctata attactaaaa tacagaaaaa tgtacaaaaa
     2281 ggaatagatg gaacacaaag tggaataaac ggtattgaag atggtgaaaa taaactaaat
     2341 agtggtatta aaggagttca aactggtatt gacaatatgg taaaagctag agctggtatt
     2401 aatcaaggaa tagatggtgt aaaaaaaggt attgctggtg ttgataaagg tataaaaggt
     2461 atggaacaag gtcttgaaaa acaagatgag gccatcaagg aacttacaac tgcttataat
     2521 aaaataccta ctacagaaaa aactcctact gataacgaaa ataactcaaa tgaacaaaac
     2581 cagagtaata ccagtaattc tgaatcagac gacactaaga atgattctac agaaaataag
     2641 aataacaatt ccagcgagaa taaaccaaat gtaaatgaac aaaaagaaag tttaaatgct
     2701 caaatacagc aactaaaaaa gacaagagat gacttaaata caaagttaca aaaaaataaa
     2761 actcaaaaga acgaattaag taaaaaacta agaacaatgg aatctcaaaa gaaagagcta
     2821 caaagcaagt taaataattc tataagccaa aagaaagaca tggaaaaaag ccttgataca
     2881 atgcaacagc aaaaaaaatc actccaagca gttttagcaa agacacaaga ggtaaaaact
     2941 gaaataccaa aggcttttga taaatcaaaa ttggattata taaattccat agaaaataat
     3001 agaacaaaaa tagaaaacac actacaatct actctaaact caggatttaa acagatgtat
     3061 ataacagtat tctgtgtaaa tctactagca tttataatat tattgttcta taaagaaaat
     3121 aaaactagat aaaagagtgt tatagtatta tattgtaaat ttatcttatt cttaaatatt
     3181 ttattctaaa gaataaattc ataaaatttt tgtaattgat taatataaat tacaatataa
     3241 tactattttt ctcttaatct ttttctcttt tacagacaat caaaaagcaa tcttagttta
     3301 tcgaaataaa tttatacaat ctattcatac acccctttac atttatataa caattaaacc
     3361 tattgttata tatacaaatc aaaataaacc atatattttc aactataata tgttaaaatc
     3421 atattatgag catattaatt taagcaaaat gtcatttaga ctttaattta gtagttttat
     3481 attataaatt tatatatttt taattttaaa tgtcatttag acattactca taaaaagaaa
     3541 ggagtgtatt ttgtggatac tttaggaaaa agaattgctt atttgagaaa ctccaaaaaa
     3601 cttactcagc gcaaacttat ggatatttta aaatttgaaa acttaggcaa gtatgaaaca
     3661 ggagatagaa aacctaattg tgatatacta atgtcaatag ctgattattt taatgttacg
     3721 actgactggc ttctctatgg taaagagaag gtaaatgtca atagtagtgt aaaagaagat
     3781 aaagaagatt acttacatgt tactaatgat gaaatgacga tattaaatct atacagacaa
     3841 ctaaacgaac gtgataaaat aaaaatagaa ggaatacttg aacttaagat atctgaatat
     3901 aaagacttaa aaaagcattc ctctaataat aatgaagata aaatggttta aacatacata
     3961 atttgatacg attttacatc atctattaac cttctttttt tgtagtatat tactaaaata
     4021 tggatattgg attatttaaa aacaagtata gtctaatttc aagcacattt ataatattta
     4081 gattatctta tccatatttt agtttaagta ctttgaaaaa tataatacat ctctatgtat
     4141 aacttatatt cctaagaaat gcattactca tgtttttaat tcttccaaca tctttctata
     4201 cacaattgta catccttcat aattcccctc tttaagaagg attgaacatg tactagaata
     4261 tttagttaaa gtatttaatc tttccatatt taagcttttt atattactga gtgctgtaat
     4321 aaaatcatca attgctctac aacactctat aaccccatct gaatccaatt tacacacaag
     4381 aatattcacc tactttatat actatttatt aatcatcttc tgaatattct atattaagta
     4441 attgataaat tgatgattta tattttttac ctgttcttct tccatttaaa atagctgaca
     4501 aatagttttc attaacacct attttcttcg ctaattccct ttgagtcata tttaattcaa
     4561 taagtctttg cttgacttgc actcccaaaa aaggtatcca tttttttcga gcatccattt
     4621 atcaacttcc tttctaagtg atttatattt acttttatat tattttagct tacacacccc
     4681 ctctatttag tatatatttt taatatgtac acatattaag tatataaatt tatttaatac
     4741 taaataagat tttttgatta ctttgtgcta aaattaggat gtaaattcgt atcattaagt
     4801 taattataaa gtcattttga ccttaagtca atgcttttta tgtcattttg accttaaatt
     4861 tgaaactatt tacatcttaa aaagtctttt tgacattaat tgaatggaga gtgtttatgg
     4921 atactttagg agaaagaatt gtttatttga gaaaagcaaa aaaccttaaa caatatgaac
     4981 tagaagaaat gttgggctgt gacaacttaa gtaaatttga gagaaacatt agaaaaccaa
     5041 attatgaaat attaaaatct atagctgaga tatttaatgt ttcagtagac tggctattaa
     5101 atggagataa tctatcacat aaatcagatt taatttgcga ttcctcctca aactatccac
     5161 ttaatagcat aaactctaat gaaataaagc ttttaaacaa ttttagaaag ttaaatgatt
     5221 atgataaagc caaaatagaa ggtatgattg aattaaaact tcatgaatat gaaaaagaga
     5281 aggatttagg aaaaattgag tataacaaaa ataaagatga gaaaatagat aaataatttt
     5341 tatgttgctt ttgatacgat tttacatcat ttctttgttc taatattttt atttcattta
     5401 ttttcttttt tataatttta tgataaaata attagtatat tttgctttac ttgaatgtaa
     5461 ttatttatac aaaagtcaaa cataatataa atgtagtaaa tttttagttt caagtaatat
     5521 ctcgttatta taactaaata tttattaaaa ttaggaggat aaaatgggct taagagacaa
     5581 attcgcgcaa tcatttgcta gatcaaaaac tatgagtggc cctgagaaaa aggctaatga
     5641 aataatgggt aaattgcttc ttaagaaagc tatattacct atcgttttaa tgttcgtgat
     5701 aataatcgct ggagctatgc ttaaaatcaa ttcttgggtt acacttggca taaatttagt
     5761 tattgctgtt ggtgctttct tctacataag aaattctagt aaaaagtatc agaactttaa
     5821 accttatgtt ggcaatttga ttagtttaga gaaaaaaggt aaaaaagagt acgttgctat
     5881 tataaaacaa ggtaagttac cagttaagtt acaaatagct tatggtggtg aagaccttga
     5941 acatgttaag aaaaatcaaa tggttcaaat aagttataat ccagatgcta aaatagctat
     6001 tttggttaat agacaataat ttaataaaaa aaaggagttg tcttaaaatt gattttttct
     6061 aatcaaattt aagctcaact ccttttttaa tttttatata ttatttttat atattatttt
     6121 tattactttt tttaattaat atttttaaat atcaaatcct tctcaataaa atagggtact
     6181 atctacaata ctactttatc ttttcctaac aatatataat ctggtgctac tctttcatgt
     6241 tatacaccgt tttgtaacat accttttata ccagtaacat cttccttaat ttcctctaca
     6301 tctgttttca ttatacccat ttgaacaagt atatttttgt ttatttcttg ttgttgtgta
     6361 gataattcta taaaattttc tacagttttt ttatacatgt ctctatcttc ttttttctcc
     6421 tgcatagtat ttttaaataa tagagcacat agtataccta ttgcccctag actactcaat
     6481 tcggttatta atttatccat tatagcctcc tattgtctaa attttttata cttatagctt
     6541 taatttttgt tatattctta catcatgcaa tattatattt tcaggattat caatttaatc
     6601 ttctgtactt taaattgtag gtgcaattta tagattgaat aaataataaa tatatataca
     6661 atttctaata taacaaaaaa taagtacaag gcttacttat taaagttaag accttgtact
     6721 ttacataaaa aaaataacta atacatagtt attctaaatt tactattcaa ctggtatttc
     6781 taattctttg catctaattc taacttgctc tctaattact gctggtactt cttctattgt
     6841 ttttttgcct cttaaaatta aagctacata tacttctacc attccactct cacctccaga
     6901 ttttcttaca ctaaaagagc ttatattact tgcttcattg acagaacttt gatttaacaa
     6961 catctcatat atttctgcat ttgctataaa attatttgtt atatcatcat cttgctttac
     7021 ttgacctata cttatgtctg aaatgtcttt atactgcatt tctacttttc taccttgttt
     7081 gatttgttct attttatttt tatcgaattt aaacaatttt cacacctcca ttttaagctt
     7141 gtggctcgtt gctttcagca tcatatctaa aaatcataga tggacgtttt atagctgtcc
     7201 atttatctcc tttttctact atgattttag gctgagcact ttttatgttg gcttcttcta
     7261 attcatagat ttcttggtaa cttgagtctg attttttgat ttcaactagt ttcatgtctt
     7321 tgaataagtc agatttattt atttttactt gaactttagt tttatttatt aattgatata
     7381 taggattgtt ttccatcatg tcatccttta atccctcatt taatgttact attaaatcag
     7441 tgttattttc agtaatgttt atgattgaat atactttatt gtttatcatt aaaatagaat
     7501 ccttttttac tttaagccct gtttctgatt taaatataaa catacttgaa tataaatcgc
     7561 ctgtaggact actggcttta atcagtatct tattttctcc taatactatt ttattttgtg
     7621 gtatctcaaa tataatgcta tttatttcca aattagtctt agtagatatt aattcatcat
     7681 ttataaacat ttcaagtttt tctataacat tattagggtc ttgtactaca actctcactt
     7741 gagatattga tttttcaact atttctatag atgctttact tgtatctact tttgttatca
     7801 tgtatattgt atcatctgaa agctctatat ctaaaatatc atttagatgt tcaggaattg
     7861 gtattttagt aaattcatat aaatcagtaa ttgatataga tattttatac atagatttat
     7921 ttttaccagt tatccataga ctattatcat ttttaattac tagtatgtgg tcatcattat
     7981 gtgacttaat ttctttaaca ttagttaata ctttagtaaa agttcttatt atcttatctt
     8041 tttcactttc tcctagtcca aataatccat ttgtattgta cccttgagcg tacaactctc
     8101 cctcattagt taaaacaagt gtcattgagt tatatatact tatatcttta acagaaggta
     8161 atccagtaac cttttgtaac tctcttacat cattattgtg gccaactccc aattggtact
     8221 cagtattctt tccatatgac catagagtat tatctgtctt aataatatag gagtagttat
     8281 ctccacaagc aacttttttg acatctgata tatttacttc agtaagagtt aataattcta
     8341 tatcttcaag ggtttgatta atttctttat atgaattact tcctattcca tataacttgc
     8401 catccttagc aacagccaat gaatgtgttt ttgatatatc aatatcagat acctcaaatg
     8461 gcaatattat tgaagtatac ttaaaattat ctttaaatat gtttttataa tttatatact
     8521 tattatttgt ttttatatac ttatcattaa acattactag cttttcattt ttcttattaa
     8581 aaactatttt atctgatgtg ttatctgttt taaaatatga atgtagggaa ccttcaagag
     8641 cttcattttt atcattatca tatctatggt gaatacctaa atgattatat ctattatctc
     8701 ctgttgaaga ataaaatttt ccatatatat ctttaataaa tatagtattg taactagaaa
     8761 atacagtatc aacatctttc tttatagata ctttataata tggaactgac atatccctat
     8821 aagattcatc agatatacca taagtatctc tacctgatga tatcaaattg ttattattat
     8881 ctatataaag tgtattatta gctgttacaa aaacatcttt aacatcttcc acactatttg
     8941 tattattatt atttgctata cttccatatc cactaagacc tccaacaaat agtttattgt
     9001 catttgttaa aatggctgaa gtatatccat taactactat tttttttgca tttataatat
     9061 tattaattac tcgtataccc tttatatcat tagtttctgt tgaaaagaat accctctctt
     9121 ttttccctgt agtataaata acattgtcat ttgttataaa aaatgtagct tcacttcctg
     9181 ctgttacaac agattttaca ttctcaatat ttatttttgt aaatgtactt atacttcctt
     9241 cagtaaaccc aaaatatcca tttacaaatc cacatacata acatgtatta tcatttttaa
     9301 caattattga tacatcatta catatataca catctctaac tccatttata ggtatacgtg
     9361 taaatatatt cctacttgta gtatcaccca atcctaactg tccttttcca ttattacctg
     9421 ctccatataa aagaccatca ttagttaata atagagatgt gtttccatat acagctacat
     9481 attttacatt ttcaacattt attttagtaa atacatttct atttacatta tctcctattc
     9541 ctaactgacc agaattattt aaaccaacag cataacaggt attatcattt tttatcgcaa
     9601 atacatgttt actattgtca gtaaatctat ttacatttat agattttata ttatcaatat
     9661 ttattttagt aaataccttt ctatttatct catctcccac tcctaattga cctacatcat
     9721 ttaaaccaac actgtaaagc atattattat tagttaataa atatgtagac ttcgaatggc
     9781 tatatatttc ttgtatatta gaagatattt cactactgct attcttttta ttgtaattta
     9841 tcttattatt taaatttaaa ctactattaa ctgatatacc atgcgtaccc ttagagttat
     9901 atccacatat aaataagctc ttatcagtta atattgcaaa tctagtttta tctctactta
     9961 aatcataagg aggtataaat atatttttta tatttttcat taccttttta taattagtgg
    10021 cattatatcc atcttgtgta tctgaaacat ctagttcttt tgcactttca gatatattcc
    10081 caaaaccata taacgtgtta gaagataaat acaatagagc tttatataaa aatatggctt
    10141 tatcagaggt aataggacct gaatttatat tagatgctgc aatattatta gatgattgat
    10201 tgttaagata attccttcca tattgatgta tataagatga acctccagaa ataagtaaat
    10261 tatctggatt tacatttttt aaattacttg ttgattctcc ataatacaaa taggcaaaat
    10321 ttgtagaata tgatgattgt gtacaccttg tgggataata gttatatatg taattactaa
    10381 tatatccagc atgtgtaatt ccttgttgtt gaactctata agtagaatca cttggctttg
    10441 tccataaagt accatggtat ccagttaaaa aagaaccata atttgtacat aatatagcca
    10501 tatattcata tataataata ttattaattt tagtaatatc atttgtacaa gaatttgtac
    10561 tataacttgc tcctgaagga tttacataaa accagttatt tattaatgtt aaatttatat
    10621 tgactccatc attaggatta acacccaata aatagtttgt atttatacca gttgcatata
    10681 cctttttttc gtcagccaca ataaatgttg cattatatga actatatgca tctgttacat
    10741 tgctaacacc aatctcagta tattgacttg ctgatttatt actaagtcca ttaacataac
    10801 ttgaactctt tccagaagta tatatttgct tattattagt tacaattaat gaaaaccagt
    10861 ttgataaatc agtatttata acaacttttt ttacattatc tatagatatt ttcttaaata
    10921 tatctgaatt agaaggaaga acacctgcta ttgagttact atttggccca gaataataac
    10981 aagtgtcatc atttttaatg tagaatattc ctccagaaat attttttgat gctactatag
    11041 actttacatt agagatactt aattttgtca actcatttaa ttgacttggt aagtcagaac
    11101 ccattccttt gtatctatta tttcctagac catatacatc tccattccct aaaataacca
    11161 gagtacaata aggactaaaa acgacatctc ttacagaatg tatatctagt ggaagttcaa
    11221 tatcctttat ttccatttct tcttttaata aagttaattt atcctcagat ttaattaaat
    11281 ttatataatt actataacta tatttttcac tatccatttt cttaaattct acaatgtgca
    11341 tatcatcaaa aaacgaacct aattgtccat aagtattatc tcctgtacaa tatgaatcat
    11401 tatttgaatc aattagtatg ctatgtgata aaccaatgaa aatattctta gctacaatat
    11461 tttttaaatc tataaatgaa ttatctatat tctcagagta atttgctata cctaactgat
    11521 atatatcatt atttccagca accttcaaaa tacctttatt atctataagc atagtattac
    11581 cattaccaca tgcaatagtt tttatattta cttcttctat cttagtaaat agtgatgatt
    11641 gaagtgcatt tccaaaacat ttttcctttc cacaaccata tatatcatta ttatacgtca
    11701 ttaacacagt gtgttcagaa ccacaagcta tagtcttaac atcattaata gaaactaagc
    11761 taaataaatc tctattattc ttatctccta aacctagttg accttgatta ttaaaaccag
    11821 tagaataaca tttattgtca ttctttatta taaaagctga tttattatat gagaatacag
    11881 cctttacatt atcaacatct acttttataa atgtatttac atttgcagat gctccattag
    11941 ttcctagacc taattgacca tacaaattat ttccagcaac atataaagtt ccatcattgt
    12001 ataaaagata tgttgattga agtccacatg caatatcctt tacgcctttt atttctggaa
    12061 ccttctcaaa tgtggaaact gtttttccta ctttttctaa tcccaatccc atttgaccat
    12121 ctgtatttat acctgcgaca agtaattctc catctgaagt aagtataact gcatgagatt
    12181 ctccacatgc aactttttta atattagtag atatatttgg tattttcgta aataattttc
    12241 ttgttgtatt gtcttctccc aatccaagtt gaaagttact attatctcca catccataca
    12301 agatattatt attttttaga ccaaatgtat agttacaaca tccagtaaaa aaattaataa
    12361 agtcataatt atcataccct tcatcaaatt catcaactac catattttta tcgccaaaga
    12421 aattacctct ttgaagtagt ttagttcttt tcattataaa aacctcctaa ttatcttatt
    12481 tcatacgaat atttatttaa agttatatct gtctggccaa ttgaatatgc cctattagat
    12541 tttgtttcaa caaagttatt atccatttca tgtatatatc taactctatt tgcattaaat
    12601 ggtagaacca cattcttaaa tgtaacacta ccaagttctc cagttataca ctttctattt
    12661 aaatctaact taaggccttc tatagctatt atgtttttag cattagttaa ggtatcatac
    12721 caatatccag cttcatctac atctatttct gactctaatt ctaagtatat taaaatatct
    12781 aatacttttt cagataattt attgtaacta ttttttaata cagatagttc agtttgtatt
    12841 tgacctccta ttacatcatc aatagatata tttttaattt tttctactac ctcatttata
    12901 gctcctgata agtttttagc ttctgtagat aagagttctt ctccaatttt tacttttatt
    12961 tcttttatat ttttatcaac cttatcatct aaatcttgaa tatccttttg agttacaaaa
    13021 accaacgtcg ggtctacttc taatgtaaca tttgaagtat tggacaattg taaaatcatt
    13081 tttacaacca aatctttaat actgccatct tcagcacgtg gcttatacgt ctctggatac
    13141 ttacctatag ctattatatt attctcatta tccagaacag cagcttctct tatcataaat
    13201 ccaccaactg ttcctggtaa aatagtctct ataactatcc aattaggatt tttttcatca
    13261 attcttagag aatttatatt tccttcccaa actacattct ttaaagcagt ttgttcctct
    13321 gttggctcat aatattctcc attactatct ccaacttgaa acttggttaa tccaacaagc
    13381 tctcctagtg cagttgcatt tgctattttt tctctaccaa ttttggttaa tattatataa
    13441 aatttttctt ccaattatac cacctccgat ttaggataaa ctactacatt ttctacgcct
    13501 gtatcatttg caatagctat attaatctta caattatttt caatgcattt aatgcttaaa
    13561 ggataaacct ttatttcttc tctacttaac attccagctc cacagtttac aacagcgcta
    13621 cattttataa ttggttcaaa gtatggatga acttttactt cttcactaca taccatacct
    13681 ccaccacaat agaccatact tttatctagt attattggtt ctaaatagtg taataagtga
    13741 gcaggtttta ctctttcaat catatcgcta caatctaaaa gagttttata gtcacaatta
    13801 tttgctataa aactcaatac gaatgtaaat tcatcactat aaacttttat atctgtttct
    13861 gattttgtat atgcctcaca tatacttttt ataacttcaa tagtactagt acctctgctt
    13921 ctcattttag cttttatatt gctacgtctt gtgtcaaaat caagttcttt tttttcaatg
    13981 cagagtatgt cttcccacat atctaatccc catgttgcag tgtcaacaaa taattggtta
    14041 aaggtatcat caatatcttc tttaagaata tttaattctg tatcgtatgc accttgtatc
    14101 tcctctacaa tgtaatttct atcaaatgat ggtagtttat caattagctt cattgatttt
    14161 ccacctcact aaaattaaca gttgttacac taggtatttt atcttcatta atagttatat
    14221 tatctgtact tccatttata agtaggttac ttaaatcgtg tacaccagta gtatttataa
    14281 gtattcccat tacttttgta tagattatct ctcctctaat atctctaaag tatgtattta
    14341 tactttctag gaaagattct tttacattgt ctaatgtata tccatcttct aatttcatta
    14401 ctgcacttat atttatttct attggtaatg gtgtcacaac tgttatagtt ggtccaatag
    14461 gcttctcttc atctatatgt tgctgacacc tttcaattgt ttctgtatca acagcttgat
    14521 tatttttccc aaatatcaag accttaactg ttcccggacc atcccatctt ggataaacct
    14581 taacattata gactccatct acttccaaag cccattcttc atagtgagct ttatttccac
    14641 ttgtagcttg atttctttgg attttataaa atctttcttt tagttcttca tctgtctcta
    14701 tatctgtacc accttcaaaa cttcttgtgt tagtaatctt tgttactcca gatatattat
    14761 caactagttt aaattcacaa tttgtaggta aattatattt cttaccaact tccagagctt
    14821 gaactgggct attgtcacct tcttcactac caatagttac atcttttatt actacaaata
    14881 gtaaatctct atatgatatt attgtgccat tatttattac agttcctttc tctccaataa
    14941 attcaacttc tccatttgac tctgtaccta actttctata tacaccaaat tcattgactc
    15001 ttttatcgag aaactggtta tatgtgtctt gtataaaagc cattttatgt atctttgaaa
    15061 gttctagata tatctttgaa agttctagat tatttccaga taccatgttg tttagaaaag
    15121 aaccttctcc tttataaata tcaagattaa tattttcaag agttctattt tttattactt
    15181 catatgtctg gtcactatac atttatttcc acctccccat agatagtgga tacttttaca
    15241 tttgcactca atacgtctct gttaaagtta gcacttttta cgtttacttc taatatatat
    15301 ggatttatta gaagggcctc ttttatgaat ctactagctt cactttctgt aagacctttt
    15361 gtatattttt gtcctattag ttctgaaagc tctgtcccat attctaaaga gtatatctca
    15421 tgttcatatc tatttgtctt tatacacttg tatatccaaa cttttaaggc ttcttttttt
    15481 tcaattattt taaagtcacc tttttctaaa ataggttcat ctttttcaaa atcccatgcc
    15541 acttcacgaa agattggcaa ttcttctgtt ttaggtaaga tataatcctc tgggacacct
    15601 ataaaaggaa atattgtact cattataagc tcaccaactt acttacaaca gcaaattttt
    15661 cgcctattct aaacattatt accatatccc cagattcaaa attatctata aaaggatttt
    15721 ttacttcatg ctggtgttct tgatttgttt ctgtgtcaaa tgtttctagc tgtctatcaa
    15781 tcatccaact atctatcaaa atatcttctt tttctaatat gatgttattt gtctctattt
    15841 ttaaatctgg taatttactt ttaatttttc caataaaaaa agaaggttga ttatgatact
    15901 ttccttcttc tcttattatt cctataaatt catttattgg attagccatt atatcaccat
    15961 ccttttataa tactctccta gctgtattaa aatctcttct actacttaac ttacttattt
    16021 taactacatc acctttttgt ggagcatgta aaaattctcc attacctata tataatccaa
    16081 catggctgac tggattatga aaaaatacta aatctcctgc ttgaagattt ttttgttcta
    16141 ctttcttgcc ttttttagat tgttgatttg acgttcttgg caaactaaca ttaacttttt
    16201 taaaacaata gaccattaaa ccagaacagt caaaactact tggtccattt ccaccccatt
    16261 tataaggttt acctagatgt ttttttgctt ctgatattac tattttagct ttatcagata
    16321 cattaccaga ataaccacca ataataattt ttccttttct tcttccaaat ttactagctt
    16381 cttcaacatt tccaaataaa atatctatat ggtatgttcc attcttttct atagttattg
    16441 caggtcctac gtcatttact ttatagactc catcacgacc agaaactcct gtaattacct
    16501 gtatttcatc tccatagttt aataaaggat gtttatttaa aaactctttt gtataataag
    16561 tttgttcata tttaccaacc ataggagcag cgcaagtttt tttagatggg tcaagttttt
    16621 tctttctaca atctgtatct ccaccttctt cttttctagg acagtaagct gtaaattctg
    16681 ctgtaaactc ttttcctcct gcataatctt ctccccctaa gttactttct tccttaggtt
    16741 catcctgtcc tgctgacttt tcatccataa gattttgaaa attaagttca agctcaattt
    16801 gatattctcc attttgccaa gtatgtttgt ctgtatctat ataaaatagt cctacaagct
    16861 ttgtataaga atctttaact tttactcctc taccagttat acaacttaca tctccataac
    16921 ctttaagaga acagcttttt tctatcccat taaactcgct atcaatatct acatcttgat
    16981 tttcttgttg ctgaattact ttttgcatta ttacatttac ttccttaaaa atttcattat
    17041 ctattttttc gctaatcttg cttccatact ggtctaccac tattacttta ttttttacat
    17101 tttccatgct ctccgaaaag gtggtattta taatattaaa tccctcttca aacataacat
    17161 ttaatgtaac agttcctttt tcaataacat taaacttatc taaattggcc tctatcatat
    17221 actttttctt tgtcttttta cttgcttctg tatatgcact cattattgtg tcataaccat
    17281 ttactcctat aaacatcttt gtatacttga cattggtctt tgctattgtt ccaactgaaa
    17341 gcctattttc agcaaatact tgctttgcta tgtcttcaac taacttatct ttaaaattgt
    17401 atgacacttc actttgtgta agtaaaaatc ccatatcttt agatacaaaa ctaatttcat
    17461 tactgcttga atctttagac ctattaatta tcattcctct aaagagttct ttctcatcta
    17521 catagaaaca gactgtacta gctattggta tatctatttg ttggaaattt acatcaaatg
    17581 atgattgtat tattgaaaac tctagtgtcc ttgatggaga tttataatca cctgaccatg
    17641 ataccttgtc tactatgtca gttatgtcat atatacttcc gttttttatg tgtacccaaa
    17701 tcttaataat aaaaaatcac ctgccttttc atttaattct ataagcagtt atttataact
    17761 tattatctat aatagttatt gttttggagt tacttttggt atctttattc ttttatactc
    17821 ttttaaatct aatgtaaaat atacatctcg agtcccatct ctttcttcat aactaaaccc
    17881 ttcaattatg acttccatat ttatatttgt ttccgtaatt gtaaatctta atataaaacc
    17941 ttccttcatc catttttcta tcttatttac acaatcatat ggtgatggaa aacctgtata
    18001 gtcacagaaa gtatagtctt tatttggaaa aaaacttgat attgaaatgc ttttaagagc
    18061 tacaccacca aaagttgcta tttcacctat tttgagtata ctagaagagt ttacagcagc
    18121 ttttccatta atactaaagg aagatggaaa tactggaaat ctaaatctat cttcagcttg
    18181 tctaagccac atttccatta taagacaccc cctaactgtg ccaattggat acttgcaact
    18241 aattttgatg ttatcttttc tatatcagct tcttctctaa tcactattgt atcagctaac
    18301 ttagctatgt ttatatttcc accatttttt ctgcttctat attgatttgc ttcttgttta
    18361 gttaaaacca tttcaccttc atggagtctg gtttgatagt cattataagg aacatagttc
    18421 aatccaaatg cattattacc tttcttacct gaataagctt taccagaatc accagccttt
    18481 gttccagcat atggattatc tttgtaattt tttttactct tttccactag atttaaaact
    18541 gttcctgtga tgcttccatt ttttatctta ttaatcatag tttgccacca cttgcatata
    18601 tctttaacta ttttacatac tttatctaat atgttaaaaa aagctcccaa tattggctct
    18661 ataattttcc atccagtttc caacagaggt cctaaggtct cccatacagt tttccaaata
    18721 acacccagtg tctctataaa attgtttatt tgaggtgcat attcacctaa aaaagtaaaa
    18781 attcttgtga taataggact aacagcttca actacaggtc cgattagttg gaatgctgta
    18841 gttactattg taccaaatat acttattaat ccttgtgcaa ttggtgattt attgtttata
    18901 ctatctagta aattatttac taagtttact actggaagta atgaatctat taatgctgta
    18961 aaatcgatat tttgtaatag tgtacttcct gcgttaacaa ttgcttttgg tgcattccct
    19021 atcattgttg ctgcatgatt ttcaaaacgt tcttgcttat tttcaaccat cattttacgc
    19081 ttttcagcct cattagaaat tcgatattga gtagaatcag aaaactcttt tccatttttt
    19141 acacttctag attgcatctg gtcatatttg ttgccaataa ttatcttacc attcattaca
    19201 ttgcctattt taccagccat tttttcagat aagccagcta aattgcctaa tccactttct
    19261 ataaaagttt ttattttctt tagtccttga cctaaaatat catcaaaagg tttaacaata
    19321 ctcttcaagc cattgttaat gcttgtttga gctcctgata ttatacctgg tattgtatta
    19381 ctcatttctt tagctaaccc catctgcttt acacttatgc cttccaatgc ttcatcaaaa
    19441 gttttataat tttctcctac cagagtatta agagattcca tgtttccttt tgctgcactt
    19501 aagaaagctg ctgatacatc ttgttcacta cttgtattca tatttaaagc tctaacatct
    19561 accatagctt gagttatatc tttttctttt ttgccaccag taatattact aatcatttta
    19621 gctttagttc ccattgcaac aacatcttca gggtcaaaag gcgtatcatt tgccattctt
    19681 actaaactgc cataataatc atcaacatct atttgattaa cttttccagt atttcttttc
    19741 attacacttt ctagaacaac tttctgattt tgttcctgac caagtaaatc aacacccttt
    19801 ttcacagcga cacctatagt agcaccattt gtcaaatcag atatcttccc ttttatcgaa
    19861 ccaagtattc cacttgccat atcctttaca gctattgcac cactccatac cttacctgca
    19921 aataatctca atttgggaaa tactttactt ataatgccac ttgccatatc ttttatagct
    19981 attacaccac tccaaaaagt atttttaaac aacattaact taggcaaaat tctacccata
    20041 gcactactca ccatatcttt aagattaata cttccttgat aaggacgttt ctctacttgt
    20101 tttaattttc ccaagatact acttataact ctcttagtca tatcttttgc ttttattacg
    20161 atattccaaa ccttgccacc aatctttttt aaatttccaa gtaatttatt tttagttttt
    20221 tctacttcat cacttttagc tttaaatatt aatataatca taatatttga attaaactca
    20281 tttttctttt gaatcaactt attaaaataa tcaataatca tagctatagt tattgctagt
    20341 atcataatac ttccatttaa acttccaata actcctgaaa cagcatttat gctagaactt
    20401 aattttgtca tagttgaact tacactacta gcagtagttt gaacttttct taacgaggaa
    20461 tttaaactgt ttactctatt acttgcactt ccaatagaat tttttatgct actagccatt
    20521 acattagtgg aaagtgaaac agccacagtt gctctcctaa aattatcagt tactgatata
    20581 atttgattca tttttggtgt gtaattatct gtaaggtaca tatttatacc ttcttctctc
    20641 acatttccca ttacatacca cctcctgtca aaggacacac tccatatcca tgtttcattt
    20701 tttcaacttc ttcctcaatt tctaaggcga aaaaagcttc aataagcttt aattcgcctt
    20761 tatccattgc ataaaaaagg gacggtctta tacctttttt cttccaataa tagtacatta
    20821 ttgtagtcct ggtatccgtc cctattagtt ttttacctct tctatcactg catttttacc
    20881 ataacccata acatcttgaa gtattctata taagttggta atttcacctg gtaataataa
    20941 ttttcttgct aattcttttg gtgttggagc cttaaattta tccattagct ccttattttt
    21001 aaataaaagc tttccatcca aatcacaaac accttctata attgtaagca tctgcatctc
    21061 cggtaagtct atatccgttt tcatattttc atcaatttta agacaagtat tttgtatttc
    21121 ttcatcttta cttattgtta aagctctaca cattacttta aattcctgcc caaatatagt
    21181 tgataatctc ttaagttcaa ctatagtact cggtctttct aagttttctg tatctgaatt
    21241 taaaagtaaa tctactatgt ttatattttt tgataatcca ttttcactca ttattatttg
    21301 ctcctttatc aatcaaattc aatcaactaa cttttttact tattaactat ctgaatttat
    21361 taagtatata tttactaaat atcagtataa aaccaaatat aaaactaaat ataaaactaa
    21421 attatatcaa gaaaatcata ctcagtaaat gtgaaaggtg cttctatctc tccttttacg
    21481 ccaacctccc aatcagctaa agttaaatca tcaaatgcta tattctttac tgctattctt
    21541 tctgctccat aagaatctgg gtcatttaat ttagatatag caacaaacct aggttcagaa
    21601 cctctcttta tcttttctcc aattaacttg tgcattcttg aactaacgtg gtatagagtt
    21661 attgagcctt ttcctttata tcctatatac tttgtatcag tacccatttg acctgctatt
    21721 ataatatctt cttttgtaaa ttccatcttt gcttgaaatt tctttacttc tgctactttg
    21781 tttccatcaa gccaaagttc tccccaagta ccactcatta catttctagc ttccatattt
    21841 gccatattaa taatcctcct atatttctat tgataaatct atatcttcca tagcatcaag
    21901 tacttttatt tttgctttta aaaatacttt agaacctgtg ttagcttctt ttatttcttg
    21961 taatgtcata taagataaat ctactccttt tgattttaaa tacgattttt gtgcttcaaa
    22021 atctatttca acagtagaat cagattctat aagtgctgat ttttctaatt cttctaaata
    22081 actttttata gctactatca ataaacattt gttgtcataa ctgttagtaa cctttcctat
    22141 atagtcatct attatcacct ttcttatgtc actatgtata atatctaaag tgtcaactat
    22201 ttttattttc tggaacattt ctcctttttc tgctgttaat tcagttaaag aatttactcc
    22261 tctagcaatt cttatagctc ctgcttcttt aataagtata agctctcctt tattaacctt
    22321 tgattctgca tcaactttcg tcatcttagg tatatcgact acatcactaa gtttagtata
    22381 agttactgat tgacttaaag gtgtacctgc tataagtcca gccaccctac ttgtaaactc
    22441 atcaacactg tatttctttt ctccaactaa cacatcttct gtagtaaaat taattatccc
    22501 ttcatgattt cctacaactt ttcctaatac agctttaacc ttaaccttat ctatatctct
    22561 aagtttaatt atccaatttt ttatagcagt cttatcagct tctactgctt ttggcataca
    22621 tagatagtta aattccttag tctctaaaaa atctaatgca gtttgaatat ctgcttctcc
    22681 ttctattaca taaactaata acttatttgg agtgttaaca tttcccatca aagctaaatt
    22741 tatatattct ttattttcag cagataaatc aactggtata tcctcttttt catgtatttc
    22801 attaagacct agtgccttag catctttaag taccattgca attattcctc tagctgaacg
    22861 ttctttaaca gttgtagcta gctccttaaa tgatatgttg atacttggta atcctatagc
    22921 catactatca ctctcctaaa tttaaattga tttcttccat attttcatat actacatctt
    22981 cttcatatac actatccaga tagtttagtg taaatgtaaa gttcaataca taaattgact
    23041 cttctttttt aatactttgt tctagctttg ttatatttaa ttttctgtct gttaccttta
    23101 tatttctgtt gaataactta tttaattcat ctgaaacttc atacattcta atactctttg
    23161 actgtggttt tggtaagtat tttatagaga taacaatatc tttgttattc gtatttaaca
    23221 ttacaggtgt acaaataact gatagtatac ttacaaaaaa acaagaccct tcagagcctt
    23281 gcacactttc atcatcaata aaaatacttt cagtaaagtt ttttttgaga atctcaataa
    23341 ttgtttctaa tatttccttg tatttaagca aataatcacc acactatcac taaatattct
    23401 tgcttaatct gataatctta ctataaatga tataagtcat ttttaaatat atatctcatt
    23461 tattcctgtc aagtatcttc tatattttta ggtaatatta ttcttttcaa tctatcacct
    23521 ccttcaatat ggatttcttt cacaataaca ttatctcaca tattttattg cctattgttg
    23581 taatggtgta tataaagtgt tgtaaaattg tcctattttt tataatatct cactcaattt
    23641 aaatatcatc tttttcttta aattgtagca agatgttcta tctaagtgta gtttcataga
    23701 aatatatgtc atattgtttt ttgtcttact attataaaaa agattaaaaa attccatttc
    23761 tatatcattt agacacgtta gagcattttc tatcttctct ttttctattt ctatatcttc
    23821 tttttccatt tttaattttc ttaatctttc ctctcttttt aacacctcat tttccacaga
    23881 agagcttatg ttatatgtgt tactagtctt ttctgtatat accattgctc cacatccaga
    23941 gtattcattc tcacatcttt ttatgtccaa gttaatattc tttatcttta tatccaaata
    24001 cttataatta tgtaatcttt tatctgtttc tttaaataat atatcttttt tgttttccat
    24061 agtacttccc cttttttata tacttattat tagcttcaaa tttacaataa tctctttagt
    24121 gcacagtttt ttactaatct cacaaccttc aagaacatat tgttctataa ctgtataaaa
    24181 aaaacttctt ctatattttg ttctaaaata ctagaaatct tcttagccat aagaagtcca
    24241 ggatttttta tgcctctttc tatttgagat atataatcat ttgtaattcc agcctttctg
    24301 gcaagttgaa tttgtgtcat acctttgcat tttctataat atttcaagtt atttttaaac
    24361 attgttttat acctccatta ctattaagaa tattttgttc ttcctcactt agattatata
    24421 gaattatttg ttctttatca atagatttta gaaaaaaata ttcttttata ttttattagt
    24481 tttatttttt agaacaattt gttataatct atacataagg tggtgttata atgtttgcca
    24541 aaagattaag agaacttaga aaggaatttg gattgactca aagagaactt ggagaaaaag
    24601 taggcgtttc ccaaagagtc ttagggtact atgaaacaga aaacagattt cctgatgaac
    24661 acattttaaa taaattggct gatgtattta atgtatcagt tgattacctt cttggaagaa
    24721 ccttagttaa ggaaaatatt gatacagtag ctgcacatag aaaaaatcca catgaagagt
    24781 tacctgaaga agcacaagaa caacttaatg attatataga atttttacta aataaatata
    24841 aaaaaaaata aaccatatga tgagcagtct atttttctgc tcttttaata taattcaaag
    24901 gcaaacatac attctatttt agggggattt ttatgaataa tttggataag ctttttgaat
    24961 tagcttctca agaagagata ataattcatt ataccactta tattgcaggt gatttagaag
    25021 gactatatat aaacaaacat ggtattaaaa ttatatcatt actcagtaat ttaaaacaaa
    25081 actcaaaaaa attgacatcc atcttagctg aagaacttgg acatcatttc actagccttg
    25141 gatactatgt atcctcttat aacgattatt atacaaaaat tattatagat aagtgcgaaa
    25201 ataaagcctt aaaatgggct tgtgagttct taattactga agaagatata ataaacataa
    25261 ttaactctgg catcacttgc gtttatgaaa tggcagacat acttaatgtg gatattactt
    25321 tctttcaaaa aagattagaa tttctatcat taaaaaaaca gtctttacaa cttggaaata
    25381 ataaatattt aatattaact aatttgccgt atttctacat atttgatcca atttcataat
    25441 aaaaatagta gtaattttaa
//
LOCUS       DAYAOX010000034        25165 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.034, whole genome shotgun sequence.
ACCESSION   DAYAOX010000034 DAYAOX010000000
VERSION     DAYAOX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.034"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(92..943)
                     /locus_tag="ACGI57_001138"
     CDS             complement(92..943)
                     /locus_tag="ACGI57_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9716223.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKNLPKI"
     gene            1160..2326
                     /locus_tag="ACGI57_001139"
     CDS             1160..2326
                     /locus_tag="ACGI57_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HFG9716224.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFAMIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLVKCVAQEWYPIEY"
     gene            2348..3994
                     /locus_tag="ACGI57_001140"
     CDS             2348..3994
                     /locus_tag="ACGI57_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFG9716225.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYVQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            4091..5464
                     /locus_tag="ACGI57_001141"
     CDS             4091..5464
                     /locus_tag="ACGI57_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9716226.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            5909..6862
                     /locus_tag="ACGI57_001142"
     CDS             5909..6862
                     /locus_tag="ACGI57_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFG9716227.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            6900..7862
                     /locus_tag="ACGI57_001143"
     CDS             6900..7862
                     /locus_tag="ACGI57_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HFG9716228.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            7887..8873
                     /locus_tag="ACGI57_001144"
     CDS             7887..8873
                     /locus_tag="ACGI57_001144"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFG9716229.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            8886..9932
                     /locus_tag="ACGI57_001145"
     CDS             8886..9932
                     /locus_tag="ACGI57_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFG9716230.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            10099..11829
                     /gene="lpdA"
                     /locus_tag="ACGI57_001146"
     CDS             10099..11829
                     /gene="lpdA"
                     /locus_tag="ACGI57_001146"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFG9716231.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            12247..15195
                     /locus_tag="ACGI57_001147"
     CDS             12247..15195
                     /locus_tag="ACGI57_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9716232.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            15173..15637
                     /locus_tag="ACGI57_001148"
     CDS             15173..15637
                     /locus_tag="ACGI57_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9716233.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            15750..16052
                     /locus_tag="ACGI57_001149"
     CDS             15750..16052
                     /locus_tag="ACGI57_001149"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9716234.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            16129..17478
                     /locus_tag="ACGI57_001150"
     CDS             16129..17478
                     /locus_tag="ACGI57_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFG9716235.1"
                     /translation="MEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGLTVGVG
                     FIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALIIPIGL
                     VVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIVLVMAD
                     LTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQKKFGL
                     FGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLPVSEAA
                     QEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVLPFADL
                     AVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFKMPEGA
                     TMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKLSIEE"
     gene            17513..17986
                     /locus_tag="ACGI57_001151"
     CDS             17513..17986
                     /locus_tag="ACGI57_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716236.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGKLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            18158..18808
                     /locus_tag="ACGI57_001152"
     CDS             18158..18808
                     /locus_tag="ACGI57_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFG9716237.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            18982..19914
                     /locus_tag="ACGI57_001153"
     CDS             18982..19914
                     /locus_tag="ACGI57_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HFG9716238.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            20188..20901
                     /gene="ispD"
                     /locus_tag="ACGI57_001154"
     CDS             20188..20901
                     /gene="ispD"
                     /locus_tag="ACGI57_001154"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFG9716239.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            20898..21383
                     /gene="ispF"
                     /locus_tag="ACGI57_001155"
     CDS             20898..21383
                     /gene="ispF"
                     /locus_tag="ACGI57_001155"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFG9716240.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    21478..21686
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21750..23465
                     /locus_tag="ACGI57_001156"
     CDS             21750..23465
                     /locus_tag="ACGI57_001156"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFG9716241.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFNRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            23587..25032
                     /gene="proS"
                     /locus_tag="ACGI57_001157"
     CDS             23587..25032
                     /gene="proS"
                     /locus_tag="ACGI57_001157"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFG9716242.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
BASE COUNT        10120 a         2681 c         4725 g         7639 t
ORIGIN      
        1 ataataaaaa agagtttatc tcaaaattga ttattctaat caaattttaa gataaactct
       61 tttttattat aaagataaat aacatataac ttcatatttt aggtagattt tttcttatat
      121 gtttaggagt gcatttaaat ttttccttaa aaattctgta aaataatttt gtattagtaa
      181 atccatgagc ttctaatata ctgcataagg tatcatttgt attaattaag tctttataaa
      241 tatgagaaat acgcacatca tttaaatact ctaagtaagt ttgtcccata tactgtttaa
      301 attttctaca aaaatattct ggttgtaagt gtgctacctt agcgattttg ttaatagaaa
      361 taggcatttt gtaattaatc tttgtatatt ctaaaactgg ttcaaatttt gacaaatcaa
      421 gtactgtttt tttcatattt ttattaccta cagatatttt aaagttagta tataattcaa
      481 ataaaagttc aaataataag cttgtaaaga gcagattagc tgcaggagga gaactaagtt
      541 taatttctct catgttttct aatataagtt taactttact tgaatttttt ttatagttat
      601 tgtcattggc attaacctta aagtcaaaat agcaattttt aaaatcaggg atatatttgt
      661 ttagaaaaga caatggtact tgcaataata tagaggtatt ttgataaata catctagttg
      721 agtgaataat tccagaatta atgaacatac actcattagc ttttaaatta taagtgtaac
      781 cctccatgtt aatttgtaaa tcaccagaag ttatataaat tatttctaat gagttatgcc
      841 aatgatttgc tacataacta ccactatcat caaatagttt gaaattaaca tctaaattat
      901 caataggttc aattatttca tgtgaaaaat tatttctgtt cataataata tcctccttat
      961 aattttaaat tataaatagt aaatttataa tgttcaatat taaaaagata atatagatta
     1021 ataaattaga taataaacat acttaagaaa acggataaaa tcaaatataa taactgtata
     1081 ttaaaataac ttaaaaaaat tattttatta taagtgcaaa atatacctat ttaaataaga
     1141 taagagggga ggattttatg tgtatataaa aggtgtaaat ttaggaggtt ggttagtttt
     1201 agaaaaatgg atgacctcaa gtttatttga aggcacagaa gctgaagatg aatactattt
     1261 accaagacag ctttcaagag aggcatatga atctagaatt aagactcatc gtagtgaata
     1321 catcacagag agagattttg caatgattaa atctatggga tttaattcag ttcgtatacc
     1381 agtaccatac tttatttttg gagattgtga accattcata ggatgtgtta aagaacttga
     1441 taaagcattt gcttgggcag ataagtatgg gcttagtata ttgatagact tacatacagt
     1501 tccaggaagc caaaatggat ttgataatgg aggaatatca ggtatatgta gctggtcaca
     1561 aaatccagaa tatgtagcgt ttacattaaa tgtattagaa agacttgcaa aacgttatgg
     1621 gatgaggcat gaattgtatg gaatccaaat tttaaatgag cctataacag aaagaatgtg
     1681 gaacataatg aatgttccaa atcggtttaa ggctgtagat aaggagatgg caagggggag
     1741 taaacctaat tcattagagt ttttacgtga tttttatata aaagcatata aggtaatgcg
     1801 tccttatatg agggaggaaa acgttatagt atttcatgat gcatttgaat taaaagcatg
     1861 gaaagacttt atgagagagg aagaatttaa aaatgttgtt ctagatacac accaatattt
     1921 gatgttagct gaagcagatg gatgtgaaca aagtattgat agctatctca aatatattag
     1981 ggaaaattat gctaaagata ttttacaaat gcaaaaatat tttcctgtta tatgtgggga
     2041 gtggagctta tttaattcat atgcttgtgg tatagataca aatggaggtc aatcacctct
     2101 aaatgggatt gaatcaaata tagataaatt atcaaaagac gataaaagag aattatatag
     2161 gaagatagca aaagcacagc ttgatgcatg gagaaatgga agtggtcact actattggaa
     2221 ttataagtta ttactagata cagttaatga agaaggttgg ataggatggg atagttggga
     2281 cctagtcaaa tgtgtagctc aagaatggta tcctatagaa tattaaacta aaattggagg
     2341 aaaagatatg attaaaaacc ctatacttcc aggttttaat ccagatcctt gtatttgtag
     2401 aaagggagat gattattatc ttgtagtttc atcttttgaa tggtttccag gtatacctgt
     2461 ttatcactct aaagacttaa aaaattggga gttatatact catattttga cagatgaaac
     2521 aaaaatagat ttgaagaagc ttccttcatc taaaggaatt tgggcaccct gtttgactta
     2581 ttgtgaagaa gaagatttat tttatatagt ttatggaatt atgaactcta tgaatgcaag
     2641 atattttgat gttgataatt atttaattac atcaaaagat ataaagggag agtggagtga
     2701 accagtatat ctacactcat caggttttga tgcgtctatt tttcatgatg atgatggtaa
     2761 aaagtggatt gcatcattag actgggagac aagagaagga tatgaaaaac ctggtgttat
     2821 atgtttagtg gaatactgta caaaaaagaa agaaatagta ggatatccaa agagaatatg
     2881 gtcaggggga actgatagag gttgtattga agcaccacat ataacaaagc gtggagatta
     2941 ctattatata atgtgtgcag aaggtggaac tggatatggt catagtgtaa ctatgggaag
     3001 ggctaaaaat atatggggac cttatgaaaa agattcaatg aatccaattg taacttcaat
     3061 tccaggcgat ttttatgaaa gacatgaccc tgaccatcta aagcctaaat actataatcc
     3121 agaatcaaaa cttcaaaaat ctggtcatgg aagctatata gaaacaacat caggtgaagt
     3181 atatttagtt catctaacat ctagaccatt tgtaccagag ttaaggtgta ctttaggtag
     3241 agaaacagct attcaaaaaa tgaaatggac aaaggataat tggcttcgaa tggaagatga
     3301 aagtaatttg gcaaaagaat acgtatcaga aagtaaacta gaagaacatt tagtttcatc
     3361 tataccaagt tttgatgact ttgattcaaa tgaattaggt ttacagtact atgctcctag
     3421 gatttcacca ttatcatttg cagatgttaa gtctagacca ggatatgtaa ggattagagg
     3481 acaagaatca agaacatcat taaataaagt gagcatattg gcaagaaaat taacttcagt
     3541 atatgcaaga ataaccacta agatggagtt ttacccagag gtacatcagc atagtgcggg
     3601 tttgataatg tattatgata atatgaatta tataaattta agaaagtatt atagtgaaac
     3661 attgggtcaa agtgcactat caataataca tcttgaaaat ggagagaaaa cggaattttt
     3721 aaatactagg ataccaataa aagatatacc aatatatctt cgtctgtatg tacaaggtcg
     3781 taagtcatat tttgaatgga gttatgatga aaaaaactac cagagaatag gaaaggtatt
     3841 tgatacaact aagttttcag atgagtactg taaatatgga gaatttacag gtacgtttat
     3901 aggattaact tgtgcagatc gtgtaaagca taaacactat gctgattttg atttttttga
     3961 atatatagta gatgaatcaa aagatgtcga ttaataaaat cattactaaa attttcagag
     4021 caaaggtaaa tcttatgctc tgaaataaga aacattagaa ggcaaatctt acaaaaagga
     4081 gtatggtatt atgaatacag aagttaatgt taaagcaact acgattagat atgaaaagat
     4141 ttcatttttt gaaagaattt cttatggttg tggggatttg gggtgcaata tcatatactc
     4201 agctatgtca gcatttttgc tgttttatta tactaattat gcagatgtta gtgcagcagc
     4261 tgtaggtagt attatgcttg tatctaggat actggatggg tttagtgact taacaatggg
     4321 aattatagtg gatagaacta aatccaaata cggtaaggct aggccttgga ttttgagaat
     4381 ggcgattcct tttgctatag cagcagtatt attattttct gttccatcaa atcttggtat
     4441 cacttctaaa ttaatttata tttttattac atataactta gtttctactg ttatttatac
     4501 agcaatcaat gtaccctatg caactctaaa ttcacttata actcaaaacc agtatgaaag
     4561 aggagtatta agtatattta gaatgatatt agccacttgt ggtaccttac taataaatgg
     4621 acttacacta cctctagttg aatactttgg aaataattta tcagcatgga ctaaaacatt
     4681 ctttgtattt ggaatagcat ctataatggt attttttata acatttactg gaaccaagga
     4741 aagagtaaaa gcagttaagc aagataggaa tgaagtaata ccatttaaaa taggtattaa
     4801 atctttattt agaaataagt actggataca aataacatta tgtttagttt gcatatttat
     4861 tgtatttgcg ataaatggtg gttcatccgt atattatgca aagtttattt tgggagatga
     4921 aaaattattt ggtcctataa atatggtttc aaatatatcc caaattatag ctatgtttat
     4981 ggttgctcct tttataaaaa aatttggaaa aagaaatgta ttaatagtag gttcaataat
     5041 attaatatcg tcaaatgtta tgtttataat agttggacaa aatcatatag gagtaatatt
     5101 tgcaagtgct ataaagggaa taggaagtgc tggaatagca tcaacaatgt ttgctattgt
     5161 ttcagatact attgaatatg gagaatggaa gactggatat agaactgagg ggcttataaa
     5221 tagtgcatct agttttgggt ttaaagtggg aaatggatta ggttcagcaa tattaggtgc
     5281 agtattatca attggtggtt atgtaggaac atcagctact caaagtgatt tagctatagt
     5341 atcaataaaa gtatgtttta tatacctacc tatatttata acaatattac aaattatcat
     5401 aatgtctttt tataagcttg ataaagaata tagtactata cttaatgagt taaatactaa
     5461 ataatgaaaa agtttcttat aaagtcttaa gtttaattaa ggtctattgt aaatgttccc
     5521 tataaaaact attatatatt tatgattatt gaagaatttt tagagaagcg aataataaaa
     5581 atataagaag actttaaatg atgaaatggt tggtttttta gaagtgattt aataagttat
     5641 tgttatttaa agcattataa gtggacaatt aatttttaat ttatggagat gatatttaac
     5701 aattagaagt aatatataat atgcactaag aagcaatttt tcaactattt atgaaggtgg
     5761 tggtacaaga aaagtatcac cttttttttg ttatacaaaa aaacagttga ctttttatat
     5821 gaaccccttc tataatgtta ctgaaattaa gtaaaatgat tgaacaaatg taaagataat
     5881 tgaaaagctt gattttggag gaaagcaaat gaaaaaaatt ggagacttaa gattaatgat
     5941 gaagtgttgt tcactttatt acgaagataa tttgaatcaa caggaaatag caaatcaatt
     6001 gggaatatca agaccaacaa tatcaagaat attaaaagaa gcttttgaac aaggaatagt
     6061 aaaaatacag atagttgatg ttttgaaaaa tgattaccaa aaaatagaga gaagtttgga
     6121 gagaaaatat aaattaaaag aagtaatagt agtagatgat aaacaggatg cactaacaca
     6181 aaaacaagaa ctagcaagag ctgtatctga atatttaact agagtagtaa aggaaaatga
     6241 tataataggt gtttcaatag gaacaacact aaaagaaata ccaagatatg tggaaaaaag
     6301 taactgtaaa aatgttacat ttatacctct attaggaggg attggagaca atgaaataga
     6361 tattcatgca aatcaaatag ccgtaagttt agcgagggct tttggaggtg attttaaatt
     6421 attacatgct ccagctgtta tgtctgactt gtctacaaaa gagaaacttt gcaaagacga
     6481 aaaaattaaa gaagtactag atttaataga taaaacaact atagcaattg tgggtatagg
     6541 aaatcctatg agtttaaact caacaataat ggcttctgga tacatgaatg aagcagatat
     6601 agaagattta aaaaaataca actcaatagg tgcaatttgt ttacaagcct ttgataaaga
     6661 aggaaaaact tctatacttg aatttaatca aagagtatta ggagtgaaac tggaagattt
     6721 aaaaaagata aaaagaacaa taggtgtcgc ttcaggagat gaaaaaatag aggcaataaa
     6781 ggcatcatta aaagcgaaat ttataaatag tttagcaatt aatcacagtt tagcacttaa
     6841 attattagaa gattgtgact aatagaaaga tagataaaag ttaacaggag ggaatgtata
     6901 tgtcaatatc aaaagaaaca ttgcttgaaa tgtataagag aatgaatcag gctagaaaat
     6961 ttgaagagaa agttagttgg ttttttgcaa gaggtatggt tcatggaaca actcacttat
     7021 ctgtaggtca agaagcgtca agtgtggcgg ctgtaatggc tttagaaaaa ggagatttag
     7081 tatcattaac tcatagagga catagtcaat ttataggaat gggaatagac ctaaataaaa
     7141 tgatggctga acttatgggt aaagaaactg gattttgtaa gggaaaagga ggctcaatgc
     7201 atatagcaga tattgagtca ggtaacttag gagcaaatgg agttgtaggt ggaggtctta
     7261 caatagctcc aggagctgca cttacacaac agtataaaaa aacaggcaaa atagttttat
     7321 gtagttttgg tgatggagca tcaaatgaag gtacttttca tgaaggaata aatttatctt
     7381 caatatggaa attgccaata atattttact gtgagaataa tttatacggt atgtcaacaa
     7441 gcataaaacg tcatatgaat atagaaagta ttgccactag agcagcttct tatggaatag
     7501 aaggaatatc aatagatgga tataacccaa tagaagtata cgaaacagta caaaaagctg
     7561 ctgagaagtg tagaagagga gaaggacctg tattaattga aagtagaact tatagatggt
     7621 taggtcattc aaaatcagat gcaaatgtat atagaacaaa agaagaaata gaatcatgga
     7681 aagcaaaaga ccctattgaa tttttaaaaa attatcttat agaaaataat ctatctaatg
     7741 aagatgaatt agataagatt caagaatttg caaagcaatc aatagaagat gcagtagagt
     7801 ttgctcaaaa tagcccaaat ccaaaaatag agtctttatt agaggatgta tatgcagact
     7861 aagaatggta aaggaggaaa ataaaaatga gtacaaggga attaacatat gcacaagcaa
     7921 taaaggaagc aatgtcagaa gaaatgagaa gagatgaaaa tgtaatattt atgggagaag
     7981 atataggaat atatggagga gcatttggtg tatcagttgg tatgatagat gaatttggac
     8041 cagaaagagt aagggatact cctatatcag aagcagctat agcaggagcg gcagcaggtg
     8101 cagcagcaac tggcttaaga cctattatgg aagtaatgtt tatggacttt gtaacaattt
     8161 ctatggatgc aattgtaaat caagcggcaa aaatgagata tatgtttgga ggaaaagcac
     8221 aagttccaat ggttgttaga tgcccaggag gttctggaac tggttcagca gagcaacatt
     8281 cacaaagttt agaagcttgg ttttgtcatg taccaggagt aaaagtagtt gcaccttcta
     8341 caccagcaga tgctaaggga ttgttgaaag cagctataag agataataat ccagtcatat
     8401 ttgttgagaa taaattgtta tacagaaaaa aaggttttgt gccagaagat gactatgtaa
     8461 ttgaaattgg aaaggcagat attaaaagag agggtactga tgttacagta attacttatg
     8521 gaagaatgtt acaaagtgtt gaagaagcag cagagactct atcaaaagaa aatataaatg
     8581 ttgaaattat agatttaaga actttatatc cattagataa agagactata gtaaagagtg
     8641 tatgtaaaac tgggagagtt ttaatttgcc atgaagcagc taagactggt gggctaggtg
     8701 gagaaatatc agcactgata acagaaagtg aatcatttga ttatttagat gctccagtaa
     8761 aaagaatttg tggaaaagat gtgccaatac catataatcc agagttagaa aaagcagttg
     8821 tcccaagagt tgatgaaata gaagaagcaa taaaatcttt aattgttaga taaggaggga
     8881 tagatatggt ggcaaataaa ataaaagcca caccagcagc tagaagtcag gcaagaaaag
     8941 acaatataaa attagatagg ctgattggaa gtggagaaaa tggaaggatt catttagtag
     9001 atgtgttaaa ttatttaaaa gataataaag caaatactac tcctttagct agaagaatag
     9061 cagaagattt aaatatagat ttagaaacta ttgtaggtac tggatataat ggaaagataa
     9121 gaaagtgtga tgtggaaaaa ttaactgcaa aagaaactat tgtatcgact aatacatcta
     9181 aatctagtga gaaaaaagag ctgaaaattg aaaatgaaaa ttcaagcatg tttaacacag
     9241 tagaaggaat atttgaaaag ccaaatccaa tgagagctac tgtagcaaaa cgaatgtcag
     9301 aaagttattt tagtgctcct gtatttacat tcaatataga agtagatgct acagaattga
     9361 aggtacttag agcaaagtta atcgacacag taaaagaatc aactggagta aaattaacta
     9421 tgacagattt aatagtaatg gcagtatcta aaatacttcc aaatcatcaa gccttaaatt
     9481 cagcatggac agatgagggt atatttagat ataaagatgt aaatatagct attgcagttg
     9541 gtttagatga aggcttatat gttccggtag taaaaaatgc aaacaaaaag tctttaaaag
     9601 aaatagcaaa agaaagcaaa gaacttgcgg aaaaagtaaa gacagggaaa cttatgccag
     9661 cagaccaaga aggtaataca tttacaataa gtaatgttgg aatgtatggt ataactacat
     9721 ttacacctat aataaatatg ccttcaagtg caatcttagg ggtaggtgct acacaagata
     9781 agtttgttcc agtaaatgga gaggctaaaa taaaacctat aatgaactta tcattaacat
     9841 cagaccatag agtaatagat ggaacagtag ctgcaaaatt cttaaaagat ttaaaagaat
     9901 tgttagaaaa tcctttatca atgcttgtat aggttttgat attttgttac tgatataaat
     9961 agtttgaata gattatatta ttataattgt ttcagtaaat ttaaagcttt tatatattaa
    10021 gaattatttt taacagtaac tatatgtgaa tatgatttgc ttaattaaaa tataagaaaa
    10081 taaataggag gagagcctat gtcagtagaa gtaataatgc caaaagcagg agtagccatg
    10141 gaagaaggta ctatagtatc ttggctaaag caagaaggcg aagaagtaaa gattggagaa
    10201 cctatactag aaataacaac agataaagtt aatatggaga tagagtcaga aggagaaggt
    10261 acacttgctg taatcataca taaagaggaa ggtgaagtat taccagtatt tacagtaata
    10321 ggtgtaatag cagaaaaagg ggaaaatcaa gaggaagtaa aagctaaata tttatctgga
    10381 aatgtttcaa aagaagatat agttgaagag aatcaaaata tagaatttaa agaagaaaaa
    10441 ataaacaaaa aagagtgtaa tcatgattat gatgtagtgg taataggagg aggacctggg
    10501 ggatatttat cagcattaaa agcagctctt ttaggtggaa gagttgcttt agttgaagaa
    10561 aatatactag gaggtacttg cttaaataga ggttgtatcc ctacaaagac ttatataaaa
    10621 acagcagaaa tattagaaga aattgaccaa ttatcaaaga gaggtgtaaa agtaactgta
    10681 gataaagaac aggacattaa gaaggccata aaatataaaa atagagtagt taaaaaactt
    10741 acagctggtg ttggcggatt attaaaaagt agagatgtag atgtgtttaa tcttaaagct
    10801 agtgttaaag aagagcataa agtaatttta tcagatggaa aagttttaga tacagaaaat
    10861 ataataattg ctacaggttc caaagtaagg atattaccta taaaaggaat agaatcaaat
    10921 ttaataataa ctagtacaga agcattagat ttggaaacag tgccagaaga attagtaata
    10981 attggtggtg gagtgattgg ttgtgaattt gcagagatat tcaattcaag aggttctaaa
    11041 gtaactatag tagaaatgga agatagagta atcccaagaa tggataaaga gttaagcgaa
    11101 tctttaaaat attctcttag taaaaaaggt ataaatgtat taactaaaaa gaaagtttct
    11161 gaatttaaag aagaaggaaa taacatatta gtgtgcattg aaggtgaaga gccaataaaa
    11221 gcagatttat gtctatatgc tataggaaga gaagctaatc tttctggcat agaggactta
    11281 gatattaaaa tagataaagg ttcaatagtt gtaaatagta aaatggaaac aagtatacca
    11341 agcatatatg cagtaggtga tgttactgga ggtgtaatgt tagctcatgc tgcttttaaa
    11401 atgggagagg tagcagcttc aaatgctcta ggtgtgaata aagaagttga cttaggtgca
    11461 ttacctagtt gtgtttatac aattccagaa gtagcatcag ttggtattac agaagaggat
    11521 gctagaaaaa aatataatgt aaaagttggt aagtttaatt ttgctggaaa tggaagagct
    11581 ttagcttctg gtcaagaaca aggatatgtg aaggtagtag cagatgctaa atatggagaa
    11641 attttaggta tacatatgtt tggatgtggt gtagcagaac ttataaatca tgcagcatca
    11701 tttaaagcat tagagatacc tactgatgaa gcaagtgagt tgatatttgg tcatccatgt
    11761 acttcagaag ctttaatgga agctttggca gatgtaaatg gagaatgttt acacttacct
    11821 aaaaaataaa tctaaacata aattattaat ttaagtctat tattaatatt aatttatatc
    11881 ataaaatttt aaaacactta cttagccata ccccttatta tgcaaataat ttatttgcat
    11941 aataaggggt attttttatg gaaaaatata gaatatagaa tccttgtagt ttttgttgta
    12001 caaaaaagat agtaatataa aataacatta tcattttaga atgatttcaa ttgtaattag
    12061 gaaaagtgat ttttttgttt ttaaaaaaag tattatttgt aaaatataaa aaaaaatttc
    12121 ttaatattta aataaaagaa tagcttaatt taaatgttat tactttataa aaaaataata
    12181 agtacaaaaa aaacaatata tggcatataa gttgctatat aaataataag gagggaacaa
    12241 ttataaatga ggcaaagaat aaaaaactta attgaaaatg aagataagaa aaatccttta
    12301 acagatgaag cgatttcaag taggttaaat attcgaagag aagatgttac ctttttgaga
    12361 aatgagttaa aaatagaaga ttcaagacaa agaagaaaag ttgttttaat taaagctata
    12421 aaagatattt taaaagaaga aaaaactata aataaaaatg atataacaag aagattaaac
    12481 agcattgggt ttacagtatc aagatttaca gtcatacaat acttaaaaga gattcaagat
    12541 tctggtgacc taaaacttga taaagagagt gatgataaaa aagaagaaat aatcaataat
    12601 agagaaaata aagatatagc atttgataaa ctcataggta gtagtggtag cttaagtaca
    12661 attataaaac tagccaaagc agcaattctt tatccacctc atggtcttca tactattatt
    12721 atggggccaa ctggtgttgg aaagagtgag ttagcagagt gtatgtataa atttgcagta
    12781 gaatcatcca gattccctaa gaatgcacca tttatagttt ttaatgcagc agattattca
    12841 gagaatccac agcttttgct atcacagtta tttggacacg ttaaaggtgc ttttacagga
    12901 gctgatgaga acaaagaagg attagtatca aaggcagatg gaggaatatt gtttatagat
    12961 gaaatacata gattacctca tgaaggtcaa gaaatactat ttcaactgat tgataaaggt
    13021 atggtcagaa agttaggaga aacaagacta acacataaaa tagatgtaat gattatttca
    13081 gcaacttctg agccaataga ttctcattta cttaatacat ttaaaagacg tatacctgta
    13141 acaattgaaa taccagaatt agttgcaaga ccattaaatg agaggtttga cattataaat
    13201 aatttcttct taatagaagc tcaaagaatg ggagcaaaca tacatataaa gtcagatgta
    13261 ttaaaagctc ttatgcttta cgattgtata ggtaatgtag gtcaacttag aagtgatatt
    13321 caagtagctt gtgctagggg actattaaac caacttacaa acaaactaaa agaagtaaat
    13381 ataagcatat cagatgtacc tggatatgta aaacaaggtc ttataaaaat cagaaattgt
    13441 agaggaaaaa tagaaaatta tgtagatggg gatttaatta tagactcaaa tcttcaaggt
    13501 gagatagaaa agaaaaatga agatatatat acttttccag atgaaatata caaacttact
    13561 gaaagaagac atattgattt attggaacaa ggtttagatt ttgatgttat aaatagaatc
    13621 attggaggag aattagaaag ttgcattcaa aaatacataa aacaagttaa aaaagtttct
    13681 attagtaaca atcttccaga tataagtcca attgttggaa aatatatcgt agagctaact
    13741 tatgaagtaa tcaaaatagc atcacaatac ctaagttcac tagaaccgaa tctacattta
    13801 tgtttagcag ttcacctaaa agcagctttt gagagattaa aagaaggaaa agtaatatca
    13861 aatgctcatt tgaaagaaat aataggaaat tatccactag aatattctgt agcagaaaag
    13921 gtagctcaat atttaaagga aagatataac ataaacttac caaaagaaga aataggtata
    13981 atagcaatat attttaaaat gagtagtaga agagatatgt ctgaacaaaa aaagataggt
    14041 gtacttctta tggctcacgg aagagtagca tcttctgtat gtgaggttac aaataaacta
    14101 ttaggaatta atcacgcaag gtatttagat atgccatttg agaagaaacc ggaagaaatg
    14161 attgaagaag ctactacaat aataaaagat attgatgaag gtagaggtgt gattatatta
    14221 gtagatatgg gttcactttc atattttgga gatattattt ctggaaagac tggaattaat
    14281 ataaggacaa tatctagatt tgatacattg ctttctatag aggttatacg aagagcagtt
    14341 ttaccagatt taacaataga cgagatagtt gatgaagtta aaggtataga gccacaagta
    14401 aaagaaaagg ttagtaaatg taataataca aaagtagatt ttagaaaacc tataatttta
    14461 acattatgta ttactggatt agggggagct tgtaagataa aaaaaatcct agaggaaaat
    14521 attaaaagta taactgaaaa ggttgatata gaacctatag gtatgattga atatccaagt
    14581 cttgatttag agataaaaca aataagcaat gataaaaatg taatcgccat agttggaaca
    14641 attgaccctc agatagcaaa tatccctttt tttagtctag tagacataaa tgataaagca
    14701 aagttaaatc aaataaataa cctaattgat acaaatcaaa ttggtgaaac aaataaaaaa
    14761 tatgaattat cagatttaat acataaagaa gatatatttg tggatttaca ggttaaatca
    14821 aaagaagaag ttctaaaata tatgagtaaa aaacttagag agaaaggtta tgtgacaaaa
    14881 aatttctata aaaaagtagt ggaaagagat aacttatttc caactgaatt atcagatgaa
    14941 atagcaatac ctcacacaga tggaatagat gtaattaggc cagctataag tatagccatc
    15001 ttaaagaagc ctattgtgtg gagtaaaagt aaagtaaaaa taatactttt attagctgta
    15061 gaccaaaagt gttttaatcc attaagcact ctactctcat ttatagaaac agatgaattt
    15121 aagaagataa aaaacattaa agatagagat tttattaggg agatgatatt agatggagtc
    15181 aatgaaaata attagtagca atttaatttt taaaaatatt gaagtgtcta acaatgaaga
    15241 tgcattaaag tttttgggac aacgtttgtt tgacgaacag tatgtaaaag aaagctacat
    15301 acaagctgtt gtagcaagag aaaaaaagta tgctacaggg ttaccaacag aaatatacgg
    15361 agtggcaata ccacatacag atatagtaca tgtaaatgag ccaggaatag caattggaat
    15421 actgaataaa cctgtaaaat ttataatgat gggtacagac gatactgaaa ttgatgtaaa
    15481 agttgtattt atgttagcag tgaaagagcc acaagaacaa ttgcaactat tagaaaaact
    15541 tatgactata tttcaagata agaatatact aaataatatt gttgacttaa gtgaagaaag
    15601 tgtgagtgat ttattaaact ctaaattgaa aaattaaaca tgtaaagtat attaaaaatg
    15661 taaaattaaa catgcaaagt atgttaaaaa atataaaaag attattaaag atttctaact
    15721 atagatatct ttaaaataaa ataatttcaa tgggggtgac taatgtggct aaaaagatat
    15781 tagttgcatg tggaacagga gtatgtactt caacaattgc tataaacaaa ttaaagaaag
    15841 cacttcaaga cattggtaaa ttggatatgg ttaatattac acaatgtaaa gtagtagaag
    15901 ttgcatcaaa agcacctgat tatgatttaa taatttgtac tacacaagta tctagtagta
    15961 taaaaacacc agtgataaat ggattacctt ttttaactgg tgtcggaatg gataaattaa
    16021 tcaatgatgt attagaagag ttagaattat aattaaaaaa attaaggaac atggtctcta
    16081 aaatcataag ttttagagac ttaaaattaa ataatggagg gtctttatat ggagatatta
    16141 aatttcattg ttggtctagg tgcacaagta atgatgccaa taataatatg tatttttggt
    16201 ttgatattag gtacaaaact aggaaaatct ttaagagctg gtttaactgt gggagttggg
    16261 tttataggtc ttaacactat tatagctttg ctaacagata atttaggtcc agctacacaa
    16321 caaatggtta agaatttagg gctaagttta tcaataatag atgtaggatg gccagcagca
    16381 tcagctatag cttttgcatc aacagtaggg gctttaatta ttccaatagg tcttgtggta
    16441 aatatagtta tgctaattac caatactact caaactgttg atgtagacat atgggattac
    16501 tggcattttg cttttacagg agctttagtg gcaggggcta ctcaaagtgt aatgtgggga
    16561 gtatttgcag cagtagcaaa tatggtaatt gtattggtaa tggcagattt aactgctcca
    16621 ggtatagagg aatatttagg tatgccaggt atatctcttc ctcatggatt tacacaggca
    16681 tttgttccaa ttgctatagt agtaaataaa ttattagact taataccagg cataaataag
    16741 attgagataa atgctgatac attgcagaaa aaatttggat tatttggaga acctttaata
    16801 atggggtctg taataggtgt aataattggt attgctgcga aatatgatat aaaaggaata
    16861 ttacaattag gtgtaactat gggagctgta ttaatattaa ttccaaagat ggcagcatta
    16921 ttaatggaag gattattacc agtatcagaa gctgcacagg aatttattga gaagagattt
    16981 aaaaacagag gcaagattta tataggctta gattcagcag taggaatagg tcatcctgtt
    17041 acattatcag ttgcactagt gttggttcct ttgactatac ttatagcagc tatattgcca
    17101 ggaaataaag ttttaccatt tgcagattta gcagtaattc catttgcact tgtattaatc
    17161 gtaccaatta caaagggaaa tgtgtttaga acattaataa taggaataat tataattact
    17221 tctggattat taatcgcaac taacttagct ccattattta cccaaatggc tttaaatgct
    17281 agttttaaaa tgcctgaagg agcaactatg atttcaagta tttgtgatgg tgcgaatcca
    17341 ttaagttggg tatttgtaaa agttatgaac tataaagtta ttggaggagt agtttttgga
    17401 gttatagctt taggaatggc tatttacaat aggaatagaa taataaatga aaataaaaag
    17461 ctatctatag aagaataagg ttaaaacaat tttattaagg agtgtatagg ttatgaaaaa
    17521 atataaatat gattctaaaa gatacttact cagtgttaca ataccaggat tttttatgat
    17581 tttaatattg atttatgcac tgtttaataa ctatacaaat tttaatttga atatatatag
    17641 tttgttaata attgtctgta cttatggtat attcaatact tttatatcat caagtaatcc
    17701 taaaaaaatt attattgata gcaaatgtat acattttacc tcatttatga ctacacataa
    17761 gtatgaaatt aaagatatag aaaagataag aataagagaa ttttataata aaaaaattta
    17821 tctaaaaatt aatgatggaa aacttttcaa aggtaggtat tggataagaa caaatatgtt
    17881 taataatagt atagcattat atagctattt cattgagttg gaagagtgct tatatccaga
    17941 ttcactaaaa tttagaaata aaaaatttga gaataagaac atataaaaat aaaaaattga
    18001 attgaagcct agcttaatct tttttgataa gatataagtt tgaactttat actaaatttt
    18061 tagaattttc aatcaagaca ataacataat agctaagcaa ttagaacaca actaagatgt
    18121 ataaggcatg tatatgatga atgggaggat ttaattaatg ttattagaga aattaagaaa
    18181 agaagtatta caagcatctt tagacctttt aaactataat ttagtgacat taacaggagg
    18241 aaatgtaagt gggagagatg aacaaactgg ctatatagca atcaccccaa gtggaatgga
    18301 ttatagaaat cttacaccaa gtgatattgt tatagtagat gtagatggaa atattataga
    18361 tggaaaatgg aaagcatcag tggatttatc agaccacttg tatatatata agcatagaga
    18421 agacataaat agtataattc atacacattc tacatactca agttgttttg ctattttaaa
    18481 tgaacctata gaatgtgcaa gtacaactct tgcaaatgaa gtagggggtt cagtaccagt
    18541 tgcaaaattt tcgccaccaa catctaaaaa gatgggaaaa tgtgttattg aagcaattgg
    18601 tgacaaaaga gcatgtcttt tagcaaatca tggcgtaatt gcagttggtc catctgtagg
    18661 acatgctctt acagccgctg taatgttaga agattcagca aaagtatatt acttagcaaa
    18721 atcaattggc acaccagtgc tactgcctga tgaagaaatt caaagagcga gagatgtatt
    18781 ttttaatgtc tatggtcagg ataaataaat tagataaaat tttaaaagta atatgaaaat
    18841 atagatattc aatgatatta aggagattta taatgatata ataagtttaa tagttaagaa
    18901 gtaaaatatg aaatgtaata gtatataaat tcatttaaga ctaaaaagaa taagataaaa
    18961 taaaaaggag tctgtatttc tatgtattac ttattaaaca aaagcacaaa tccttacttt
    19021 aatttggcat tagaagaata tctattttta aatgataaat ataatgatga tattataatc
    19081 atatggagaa atgaagaatc catttttata ggtaaaaatc aaaatccata tcaggaagta
    19141 tatcatgatg ttattgaaaa gggagaaatt cctattttaa gaagaatatc tggaggagga
    19201 actgtttatc atgacttagg taatatcaat atgtcattca tacaaaaaga taggcaactt
    19261 catgagatag attttttgga gcatactaag tttatgcaaa agatgctttc aaccttagga
    19321 ctagatgtat ctataactga aagaaaagat ttatttttaa atgggaaaaa aatttcaggc
    19381 tctgcacagt ctataaaaag aaaaaattct ctttatcatg gaactcttct ttacgattca
    19441 gatttaaata aattaactaa atatttaaat tcaaataaag caactgaatc aaatgcaact
    19501 aaatctgttt caagtaaagt tacaaatatt aagtttttac tcgaaaaaga tataaattac
    19561 tttttagatt attgtgttga atatttaaaa tctaatataa agaatataaa agaacttgaa
    19621 ttggatgaag aagatattaa aagtgtatac aatataggag aagataaata taaaaagata
    19681 gagtggacat atggtaaaac gccgaaattt caagtagtgc taccatttaa taatgaaagt
    19741 aaaataaata tacatgttaa cagatggaaa gtatcaaaat tttttatttc ttatgatgat
    19801 aatgtaatta atatggacaa gttgttagat ttgaatttct ttaaagaaga aataaaagag
    19861 agtattttag aaaattatcc acattacata gatttaatca atttaatatt ttaatataaa
    19921 acctctaatt tgataaaatt gtcttatatc aaactagagg tttttaatat tcttgcatat
    19981 taacaaccat tacaaaattg taaaattaaa aacaacacta ttgaaaaatc aatatatatt
    20041 gtaataaata tatgtgtttt tcattagcaa atagacatta actatttata caactgaaaa
    20101 tttaatatat aattaatcag atggttaaaa taaaaatatt aaaagtttta ataaatgatg
    20161 gttaaaaata aaatacgagg tgataccatg tatagtgtta taattgttgc agcgggaagc
    20221 ggaaggagaa tgaacttaga tataaacaaa cagttcataa aattaagaga aaaagaaatt
    20281 attgctcata caattcaagt tttttatgaa aatataaata tagatgaaat agtggtatgt
    20341 ataaaaaaag aagaagaaga tttttttaaa gaaaatataa taaataagta taactttaaa
    20401 aatataaaaa tagcatatgg cggaaaagaa agacaggact caatatataa tggtttaaaa
    20461 aaattagata aaaattgtga tatagtactt atccatgatg gagcaagacc atttgtcgac
    20521 catagaataa tcaatgaatc tataaaggta gctaaagaaa aaaaggctgt ggttgtaggt
    20581 gttccagtaa gtgatactat aaagatagtg tcagatggaa cagtacaaga aactccagaa
    20641 agaaatttgc tgtgggcagc acaaacccct caaacctttg aatataatct cataatagat
    20701 gcttatgaac aagcatataa gaacaattat tatggtactg atgattcaat gttggtagaa
    20761 aatataggtc aaagtgtcac tatggtaatg ggttcttatg aaaacataaa aataactagt
    20821 ccagaagatt taaatatagc agaacaaata ttaaatatgg aaaaaagaga tgaagttaac
    20881 agtagaagga gaattatatg agaataggat taggatatga tgtacacaaa ttaacagagg
    20941 atagaaaact tataatcggt ggtgtagaga taccacacga taaaggtttg ttgggtcatt
    21001 ctgatgctga tgtattgata catgcaataa tggattctat tttaggtgct ctagcattag
    21061 gagatatagg gaagcatttc ccagatacag atgaagaata taagggagca gatagtatga
    21121 aattattaga acatgtgtat aatctaataa caagtaaagg atataaaatt ggtaatatag
    21181 acagtactat aattgctcaa agccctaaga tggctccata tattgagagt atgagaagta
    21241 atatatcaaa agttttaaac acagacattg ataatataaa cataaaggca acaacagaag
    21301 aaggtcttgg ttttacagga gcaaaacagg gaatagcttc acaaagtatt tgtttattat
    21361 tgttgacaag tcagaataat taatatatta ttagtaataa gtttaatgga tatcaagtaa
    21421 taggcctata tagaatatct ataatgtatg aatttaagtg aaattaatat aaatataaaa
    21481 ttagctatga taaagaaata gtaaaatgat gaaatcttca cagagagaaa acggtggtga
    21541 gagttttcaa gaggaagtct ttgaacctgt cttttagctt aaaggtataa aaatctttac
    21601 ggtaggaacc gttatattcc caaaagagag ctattcatat agctaactag agtggtaccg
    21661 cggaaattaa acctttcgtc tctatacttg tatagagatg agaggttttt tatattttca
    21721 ggaagttaat attcaggagg aatctattaa tgaaaatgtc aaaaatgttt atgccgacat
    21781 taaaagaaat cccagcagat gctgagatta caagccatca attaatggtt aggtcaggca
    21841 tgataaaaaa aatgacttca ggtgtttata accaattacc aatggggtta agagtgttca
    21901 aaaaaattga acaaataatt agagaagaat taaataaaaa agattgtcaa gaaatacttt
    21961 gtgcagcctt acttccttct gaattatgga aagagtctgg aaggtggact gctatgggtg
    22021 aagaaatgtt tagactaaaa gacagaacag aaagagagta ctgtcttggg ccaacacatg
    22081 aagaagcatt cactgatata ataagacaag aaattacttc atataaacaa ttgccactga
    22141 acttgtacca aatacaagta aaatatagag acgagagaag accaagattt ggtgttatga
    22201 gaactaagac ttttacaatg aaggatgcct atagttttga tgtagatgat aaaggtttag
    22261 ataagtcata tcaagatatg tttgatgcat atgtaagtat atttaataga tgtggattgg
    22321 agaatagccc tgtacaggca gattcaggag ctataggagg ttctacatca gccgaattta
    22381 tggttaagtc agaagttggt gaagatgaag ttgtattttg tagtggatgt gattatgcag
    22441 ccaatgtaga aagagcagaa tcttgtaatc tagcatcaca aaaagaagaa atgaaagaat
    22501 tagaagaggt tcatacacca ggagcagcta caataaaaga gttagaagaa tttttaaaaa
    22561 cttctccaga taagtttgct aaaactttag tatatgaggc agatggaaaa acagttgtag
    22621 tcgttgtaag aggagataga gaagttaatg aaatcaaagt atctaatgcc ataggttctg
    22681 ttatagaatt tgctcttgct acagatgatg tagtgagaaa agtaactaat gcagaagtag
    22741 gatttgcagg tccaataggt ataaatgctg actatgtatt tatagataaa gaaattgttg
    22801 aacaaagaaa catagttgtt ggggctaata aaactgagta ccatataaaa aatgctaact
    22861 atggaagaga ttttgaagga atagttggag actttagaaa tgttcaagaa ggcgacaagt
    22921 gtatagtatg tggtaagcca cttgaaatag ctagaggtgt tgaagtagga catatattta
    22981 aactaggaac taagtactca gaatctatga atgcaaactt catagacaaa gatggaaaat
    23041 caaagcctat agtaatggga tgttatggaa taggtgttga aagaacagca gcagctataa
    23101 ttgaacaaca taatgatgag aaaggtataa tatggccttt gtcagtagct ccataccatg
    23161 ttgtgatagt tccagcaaat atgaaaaatg aagaacaaat atcaatagct gaaaatatat
    23221 ataatgattt gcaagctatg ggtgtagaag ttttattaga tgacagagat gaaagaatag
    23281 gtgtcaaatt caatgattca gaattaatag gaatacctat gaggattaca gtaggtaaaa
    23341 atattaatga aggtaaagta gaatttaaac ttagacataa agaagataaa gagataattg
    23401 acatagaaga aataaatgaa aaagtaaagg cagagtttat caggaataat gttaggttag
    23461 gataataact taaataagat attgtataga taatcaaagt tgtactttat agacaccatt
    23521 ttatctatat ataaggatat aaaatggtgt ttttttataa aacaaataaa tgaggaggta
    23581 attaaaatgg caaaaaatga aaaacaattt gttgaagaaa taacaaagat ggaagatgat
    23641 tttccacaat ggtacacaga tgtaataact aagactgact tagttgatta tgccccagta
    23701 aaaggtttta tggttgtaaa gccatatgga tatgcattat gggaaaagat gcaagaattt
    23761 atggataaaa aatttaaaga aactggacat aaaaactgtt attttccatt attaatacca
    23821 gagagtttat taaacaaaga agcagaacac gttgaaggtt ttgctccaga agttgcatgg
    23881 gtaacacatg gaggaaataa aaaattagaa gaaagattat gtgtaagacc tacttcagag
    23941 actataatat gtacaatgta tgcgaaatgg ttaaaatcat atagagaatt accatatctt
    24001 tataatcaat ggtgctcagt tgttagatgg gaaaaatcaa ctagaccttt cttaagaaca
    24061 tctgagtttt tatggcaaga aggtcatacg ttacatgaaa cagcagaaga agcacaagaa
    24121 gaaacaatac aacaattaga agtatataaa gcgctatgtg aagaattact tgcaatgcca
    24181 gttgtagctg gtcaaaaaag tgaaagtgag aaatttgcag gtggtgaaag aacatatact
    24241 atagaagcta tgatgcatga tggtaaagca ttacaatcag gtacaagtca cttcctaggt
    24301 caacacttta ctaaggcttt tgatattact tttgcagata gggaaggaaa tttagctaat
    24361 ccataccata catcttgggg agcatctaca agacttatag gtggacttat aatgactcat
    24421 agtgataaca gaggtttagt attaccacca agagtagctc caattcaagt tgttatagtt
    24481 cctatagctg ctaaaaaagg aaatgtaatg gagactgttg ataaaatata tgctgattta
    24541 aaagctaagg gagtagctgt agaagttgat gatagagata actatacaac tggatggaaa
    24601 tttaatgagt gggaaatgaa aggtgttcca gtaagagttg aaattggacc aaaagatata
    24661 gaaaataatc aagctatggt atttagaaga gatactttag aaaaggattc tatgccttta
    24721 gaaggattag ctgatgctat atgtgattta tttgatgtta tacacaatga tatgtttgaa
    24781 aaagcaagaa aacatagaga agacaataca tctatagttg aaaatatgga tgagtttaga
    24841 aaagctttag aagaaaagcc aggattcata aagactatgt ggtgtggaga tgcagaatgt
    24901 gaagctaaga ttaaggaaga aacaggagca actataagat gcttaccttt tgaacaagaa
    24961 aacttaggtc acaagtgtgt gtactgtgga aaagaagcag atagtatggt agtaatggca
    25021 aaagcttact aaaatacatt taaataaaaa ctggggtaga aagtatttaa tactttatcc
    25081 cagttttttg ttttttatga gtaataaaat gtatttgtaa ctatgtcata agataagtca
    25141 tagatactta tattaaaaag aatat
//
LOCUS       DAYAOX010000035        24993 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.035, whole genome shotgun sequence.
ACCESSION   DAYAOX010000035 DAYAOX010000000
VERSION     DAYAOX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.035"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(42..650)
                     /locus_tag="ACGI57_001158"
     CDS             complement(42..650)
                     /locus_tag="ACGI57_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFG9716243.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(759..3497)
                     /locus_tag="ACGI57_001159"
     CDS             complement(759..3497)
                     /locus_tag="ACGI57_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9716244.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLKDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3645..4436)
                     /locus_tag="ACGI57_001160"
     CDS             complement(3645..4436)
                     /locus_tag="ACGI57_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9716245.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4472..5128)
                     /locus_tag="ACGI57_001161"
     CDS             complement(4472..5128)
                     /locus_tag="ACGI57_001161"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFG9716246.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5121..6086)
                     /locus_tag="ACGI57_001162"
     CDS             complement(5121..6086)
                     /locus_tag="ACGI57_001162"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFG9716247.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6192..6298)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6588..7532)
                     /locus_tag="ACGI57_001163"
     CDS             complement(6588..7532)
                     /locus_tag="ACGI57_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9716248.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            7643..8110
                     /locus_tag="ACGI57_001164"
     CDS             7643..8110
                     /locus_tag="ACGI57_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6194 family protein"
                     /protein_id="HFG9716249.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8280..8879)
                     /locus_tag="ACGI57_001165"
     CDS             complement(8280..8879)
                     /locus_tag="ACGI57_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716250.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            9125..9721
                     /locus_tag="ACGI57_001166"
     CDS             9125..9721
                     /locus_tag="ACGI57_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HFG9716251.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(9819..10778)
                     /locus_tag="ACGI57_001167"
     CDS             complement(9819..10778)
                     /locus_tag="ACGI57_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9716252.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(10812..11543)
                     /locus_tag="ACGI57_001168"
     CDS             complement(10812..11543)
                     /locus_tag="ACGI57_001168"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716253.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(11536..12306)
                     /locus_tag="ACGI57_001169"
     CDS             complement(11536..12306)
                     /locus_tag="ACGI57_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9716254.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(12391..13101)
                     /locus_tag="ACGI57_001170"
     CDS             complement(12391..13101)
                     /locus_tag="ACGI57_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HFG9716255.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(13281..13433)
                     /locus_tag="ACGI57_001171"
     CDS             complement(13281..13433)
                     /locus_tag="ACGI57_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFG9716256.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(13459..15615)
                     /gene="feoB"
                     /locus_tag="ACGI57_001172"
     CDS             complement(13459..15615)
                     /gene="feoB"
                     /locus_tag="ACGI57_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFG9716257.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(15715..15936)
                     /locus_tag="ACGI57_001173"
     CDS             complement(15715..15936)
                     /locus_tag="ACGI57_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9716258.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(15949..16161)
                     /locus_tag="ACGI57_001174"
     CDS             complement(15949..16161)
                     /locus_tag="ACGI57_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9716259.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(16482..19379)
                     /locus_tag="ACGI57_001175"
     CDS             complement(16482..19379)
                     /locus_tag="ACGI57_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9716260.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDEESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKVKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(19706..20314)
                     /locus_tag="ACGI57_001176"
     CDS             complement(19706..20314)
                     /locus_tag="ACGI57_001176"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFG9716261.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(20574..21119)
                     /locus_tag="ACGI57_001177"
     CDS             complement(20574..21119)
                     /locus_tag="ACGI57_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HFG9716262.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(21237..23060)
                     /locus_tag="ACGI57_001178"
     CDS             complement(21237..23060)
                     /locus_tag="ACGI57_001178"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716263.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRILNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSILLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(23073..24848)
                     /locus_tag="ACGI57_001179"
     CDS             complement(23073..24848)
                     /locus_tag="ACGI57_001179"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716264.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         8227 a         4337 c         2446 g         9983 t
ORIGIN      
        1 aaataccttc ctatagatac atatcttacc tatataaaaa cttattctat ataaaaacgc
       61 cataaaaaat cttttgcttc ttctgcataa tctatattta tacgtttact agtttttata
      121 tctaactcat ctttactaaa tacttttttt cctttatcat cataataaaa atcacttata
      181 tataattctt cacccattat actcttacta tttagacttc tatctatttt taaagctttg
      241 caaagttttc ctggtccgtt agtaatattt tttacttgat atttactcaa ctcagtgtaa
      301 gctttgttgt atctattcat agatatctca tctaaagaag ttataggctc tacccctctt
      361 attaatacac actctggcac attttctata tttgcagata aattcaagca attatacata
      421 ccataaatta aataaacata tatatgtcca ccatctaaat ataatggctt agttctatct
      481 gtctttttat tgccatatac atgtgctcct ttatcattta cacccatgta tgcttctgtc
      541 tctatgattt tggtaacaat tactttattt tcatatttcc ttattagata tttacctaat
      601 attgatttgg ctaaatcaat tccatttttt ctaaaaaaat ccttttccaa aatttcacct
      661 cataaaattt atttaaaacg catcaaatac aactaaagta ataaataaaa ttaactctta
      721 ttaatgtgat atatataaac aataaattta gacatttatt atttatatat atcaaaaaat
      781 tctatctcaa acttttcaat attatcattg taatttgaat tatcatatac ttcttcaaca
      841 acctctttaa ttgggaatat aacttttaca ttagttcctt ctcctacctt actatctatg
      901 taaatcttcc cattttgaat ttcaatcaat gattttgtaa ggcataaacc tataccactt
      961 ccaaattcat ttgatatacc actgttttca atttgtttaa atcgattaaa tatatccttt
     1021 aatttttctt tgtctatgcc cactccagta tcttttataa ttatctctac tgtttcgtct
     1081 tttttatata tacctacaag gatttctcct ccttctaaag taaattttat agcatttgat
     1141 aatatattta gtattattct ttctatcgat tctgggtcaa gccctaccat aacagtttct
     1201 tcatctgtat caaaaattaa atctattcct ttagacttag cataactact aatagatgta
     1261 actattcttt ctgttatgtc aataatatca aaattaactg ctttaatctt atatatgtct
     1321 gacttaactt gagaaatatt tattatatta tctattaatc tcaataatct aaacatattt
     1381 tgtttagtta tttttatatg ttcttttagt atatcctgaa aatcaattat ttttcctttt
     1441 ttataaaggt tttcaataag ttgaactgaa ctatatatat tatttaaagg tgtcttaagt
     1501 tcatgagata tattagcata aaactccatc ttcatttggt catactgtct ttgttttatt
     1561 aatgcttttt taagtctttc agactctctt tgcacactta aatctttaat aattctcact
     1621 ctacttcttt tctgtccaaa gtataatgtt cttgatatca tttttacttc tttaatgtga
     1681 ttattttcat ttactatatg gatactcttc tctctttctc ctatatttaa attgtttatg
     1741 tattttttta ctaatatatt tctatatttt tgtgttgtaa ctcctaatac atcactagaa
     1801 attccgaaca actctctaaa ggctctattt gtatatagta ctcttgatgt acttaaatcc
     1861 tcaagaaaaa tagcctctgg tataacatcc agtaattttt catatattat tttttgttca
     1921 tttactgttc taagaatatt tatctcttta gttatatctc taaatgtaag caatttaaag
     1981 agtttattat ctgaccaata gtctccaact ctaacttctt caacatgttc ttcaccttct
     2041 gagtcagaca tcacaaaatt aactatacag tttgctgtgt cattgtaact tactatttca
     2101 gctttctttt cttgtgttag atttaatctt ttaaattccc tattgtcaaa gaaatctatg
     2161 ttaaaagttt caaaatattg ttctaatgtc atatcgacta aatcacttga aacacattta
     2221 aaactctttt catatttttt atttatactt attaccttat tcttctttat gccaccttca
     2281 gcacttctta caagtatagg agatggaata tttagtataa tgttttcata gtctttattt
     2341 atatttctta tttcttcatc tattttgatt tcatttgtaa tatcatttaa acttattaag
     2401 taattgcttt cgtctttatt attacttaaa cttaacaaat aaattttatc atttaactct
     2461 accagtttat caattatact tatatcgtta tcaaatttct tcaatttttc agcaactttt
     2521 tcatcttcct ttataaaagt taatatttta taataattat tcatatcctt tgaaaattca
     2581 tctttaaatt tattatttgt gtaacttata ttaaaatcat agtctgttac tatccataca
     2641 tttggtattg tatccagagc ttgtctaagt aattcttttc tctggtctat gtagtttttt
     2701 aatgttcttt gtataatcat atccttttct attgctaaat ttatctttcc taattgctct
     2761 gactttttac ttaatttttt attgattgaa tcaagttcat aatatggata cactactcta
     2821 ttattaagac tgtatacata tacataataa gtatggttac ataatagtaa atatattact
     2881 cctattaatc tatctgaatc tactatatta atcttagaaa ttgctacaaa acatataata
     2941 gttgtaaaaa aaacaaaata tgttaaatct tttattaact tatagtgctc aatgttacta
     3001 tcaagtttat tcaacaaata tagtaaatta aacatagata tacaaatatt aattaatata
     3061 ataatcatat tccagttaat atcactaaaa atatttctta taaagtaatc taatatcaca
     3121 aaagcactat aaaaaattag tattgggctt attcttagtt tttttacttt agaaatggtt
     3181 aagtatataa aagtagacat attaattatg cttaagttaa gcaatacaat ttggaatttt
     3241 tgaattttta aatttactac tcctaaaata cttacaaaaa ctaatacaag acaccctact
     3301 ttcaatatat ttataaattc atctgtttta aagctactta tataaactat agaatatcca
     3361 aatatccaaa gtaaaattat tctatacata tttatagaaa ctgtatacaa atttatattt
     3421 aaaagtgcaa taaccaaaaa taacccatta gctagtatat agaccttatt cttacttaaa
     3481 gttcctttag attccatatt ccccaccact tcttcatttt tatatagttt tacctataaa
     3541 tatgttacca caattcttcc aaaaaatttg tcactttcta gttataaaaa aaaatgagta
     3601 aatattttta attttactca ttttgtagat tttgtatatt cagtttaaaa agctggaact
     3661 atacttcctt tatattcatc ttgtataaat tttttaactt ctttactatt catagcctct
     3721 gataaagcct taatcttatc actatcttta ttattttctc tacaagctat tatatttaca
     3781 tatggagaat cacttgattc tattactatt gcatccttag taggatttaa gttagctgtt
     3841 aaagcataat tagaatttat tactgctccg tcaacatctt ttaatactgt tggtaattgc
     3901 tctgcattca tttcttttat tactatattt ttaggatttt tagttatatc ttttttagtt
     3961 ataagctctc catcttttac ttctatcaat ttattttttg ctaataattt aagtgctcta
     4021 gctccattag ttgcatcatt tggcacagct ataacagctc catcttttaa ttcatctaat
     4081 gacttaactt tttctgaata gaatcccatt ggctctatat gaactttaga tgtataagta
     4141 agtttatatc ctttctcctt cacagtttcc tctaaaaatg gaatatgttg aaagaagtta
     4201 gcatccaaag acccttcatc taaagctgta tttggaagta cataatcatc aaatattttt
     4261 acttctaaat catatccttt ttcttttaac aaaggttttg caacttccaa tatctttgca
     4321 tgtggatttg atgaagctcc tactagtatt tttttatcct ctttgtttga acaacctact
     4381 gctgatattg ctattgctga caccaatgct actgataata atttctttaa tttcattttt
     4441 actccccctc tttaaaatgt ttataattaa tttattttag tttcttataa gccagatttc
     4501 ctactccctg gattatctga actaatatta taagtgctat tacagtatat accattactg
     4561 ctgtatcaaa tctttgatat ccataaataa gtgctatatt acctaatcct ccagctccta
     4621 ctgcacctgc catagctgta taaccaagta tagatattat tgataatgta attccagata
     4681 ctattgaagg catagcttcc tttatcataa ctttaaatac tatttgtctt tttgtagctc
     4741 caaaagattt tgctgcttct atcaatcctt tatcaacttc atttaatgag ctttctataa
     4801 ttctagcgat aaacggtgct gccccaatag ttattggcac tattgcagca gtttccccta
     4861 tacttgttcc aacgattaat ctagttattg gaatcattgc aactataagt atcataaatg
     4921 ggaaacttct aaatatattt actataaatc ctaatgcact atttattgtg ctatttggtt
     4981 ttaatccatc tggttttgta actactaata tgattgctaa tataaatcct aatattgttg
     5041 ccactatagt tggtactata accatataca aagtttgtaa caatgcattt ggaaatagtg
     5101 ttgttaaaaa gtcaattaaa ctattcattt atcatttcct cccatcttat gcccttacta
     5161 gttagatatt gttttacttg ctctccactt ttatctgaaa tgtttataat aagtgaacct
     5221 aaaatatcat ctttaaattg ttctaatttc ccaaatatta tagacacatc tatatttagt
     5281 tctcttgcca tagtcgtaat aatagcttca ttagaaatat ctttagggaa tagtatcttt
     5341 atatttgttc cttttggcaa tattatactt tcttcaccta ttaattttct aagtccttta
     5401 gtatttctta agaatatttc ttctgtatct cctacctcta atacttctcc attttccatt
     5461 attgctactc ttccacaaat ctgctttata acttccattt gatgagttac aactattatt
     5521 gttattccca attttttatt tatatcttca agcaatgaaa gaattgactt tgtagtattt
     5581 gggtctaatg ctgaagtcgc ttcatcacaa agaagtactt gtggatttag tgctaaagct
     5641 ctagcaattg caaccctttg tttttgtccg ccacttagat ttctgggttt atcatttttc
     5701 ttttcagata ttccaactac ttctaataac tctaaaactc tttttttaat ttctgccttt
     5761 gagtatccaa aacattctaa tggcaatgct acattatcaa atacagtttt tctttcaagc
     5821 aatgaaaagt gttgaaaaat cattcctaac tcttttctta aatctctcat ttgcttttca
     5881 ttcaatgact ttacttcttt atcagaaaca agtacactgc cttcttggta ttcttctaat
     5941 ccgtttatac atctcagtag tgttgatttc cctgcaccac tatgacctat tattccaaaa
     6001 atttctccag attctatttc aatacttaca tctttaagca cttgaatctt tccataatac
     6061 ttattaacat tttttatgct aatcataaaa tttcctccca tatattctgc tcagttttta
     6121 tgtttatcct ctcttatgta ttacaacaaa ataaaagccc gagtataaac tcaggcttag
     6181 aaagctattt atactcatct atcaactata ttagttgcag gatttagcac caatgtatat
     6241 aaaatatact ggttgccggg tttcaaaggg ccagtccctc caccactctt gataagacgt
     6301 tatttaattt aattctatta tcatttgttg ctatgttata tatgatatat tcattaatag
     6361 tatttgtcaa taaatttatt taaatttttt attttttatt atcgttaatt aactattttt
     6421 aattagtttt ctagctccta acactttata ttttaatgtc tatataacta tagaacatca
     6481 ttataagaaa taaattaaat aacatcaatc tatctaaatc atctgtaaag taaattaaga
     6541 aaattaacat tttatccaaa ctctatccta ctattaaata aaataattta aatacaatga
     6601 tgattatata gctctagtac tttagacttt atcaaatgtt taagttctat aggttctatt
     6661 attgtagcat ttttcccaaa agttaaaaaa tattcagcca cccagttaag tgtagatttt
     6721 ggtatataca tatctatgta tccaccttta gacaagattt tcaaccctct tgccaacaaa
     6781 aattctgttt cacaacgttt tactccctca tctgtcaaat taattttaat atgataatct
     6841 gtacctactt ccatattctt aagataatca tgtatagaag ataaaacctt atatttctta
     6901 cttgataagt tttctatact caaaatcttc acaattctgt ctacacgaaa ttccttaata
     6961 gattctgatt ttatacagta agctggacaa taccaaagtc cattcatggc atacaaacca
     7021 ataggaacaa tggtacgtac actatttttt tgctttgatg aatattcaat tttaattgta
     7081 aattcattca ttactatatt aaacaattct ttcaataatg gtgttgaaca gtgcctatct
     7141 ggtgtccaaa aaacaaattt acgccttata ttctcaatat tatattgaat atcatttggt
     7201 atacaattta aaaatttttt taaaacagaa atagtttctt gctcaaatgg caaatcatta
     7261 taatattcta gtgactgata tgcaaagaat atagatttta cctcactttc tgaaaatgca
     7321 atcggtggaa gtatacgctc tttaagtaca tgatatccac cagccgctcc aacttttgaa
     7381 tataatggaa atccaatact ctccagcacc tgtaaatctc ttagtatagt acgttttgat
     7441 acagaaaatt cttctgctaa ttctcctgct gtaaaacttc tttttgcatt tattgttatc
     7501 atcagttcaa ttaaacgctc agctcttgac atatttatac tcctaattta aatatagtga
     7561 caataattgt cactattata tgataagata ttcttgcatc taaagcaata ataatagtaa
     7621 agaaagaagg gattgatact ttatggaaaa tgatactatt aaggctgatg atattctcaa
     7681 ttattgtcta tcaaacttag atgatgttgt actaatggat agttgggggg aacgagcaat
     7741 ttactacaat cctaatggtg ttttaaagcg aggggtatat gttcttacca ttaaggaaaa
     7801 agacagtaat aatgataaag gttcgttagt tagtcgtcca aatgtatacc gtgtgaatat
     7861 aggattaaaa aaagaaactt ttattgaaat gtttggatat attccaaagc gtccaggtgt
     7921 aggtcaaata gttgatatgg attttgattt tacaaaattg gacacaatca tgccacatcc
     7981 tatctactca tggatgggat ggatatgtgt cctaagccct actgaaaaga catttgagaa
     8041 ctttaaaact ttaataggag aatcttacaa ttttgcaaaa caaaaattta aaaagagaaa
     8101 aaacaagtaa aaacgtgtga aaataacctc taaataaatt ataataaaaa tattaaggtt
     8161 acaagataaa aattaatatc ttaataacat gcaaaaaagc gaagccaaaa aatgtattat
     8221 ttataaggct tcgctatatc tcaaataatt tatttcaaat ttcaaaacta tttattctat
     8281 tatttcttat tatttcctgt tattcctaat ctatcattca tatcttttat catttttcta
     8341 aaaccactac ctatatcatt tccaacatct tcagttccat gaactaattt tataaagtca
     8401 ttttgacctt ttttcatatc ttcatcagat accttatcta atttttctat tctcttatct
     8461 atatctttta ctaatctttc cccttcagcg attaggtcat cattcatttt ctttaactct
     8521 gtatcttctg ttttaccatc ttttttaaga gatgttacaa atgattgtat tttatctcta
     8581 ctatctttat atgcagcctt tacatcactt agatattttt catttcctgg atattcttta
     8641 tctttaaact gattatttat actttctgga gtatcataaa tagaatatct atttagacct
     8701 gctatataat cattatagct tgttgaataa taattcatat atccttcatt attatttaca
     8761 gattcattag gattttttcc tgtattttca cttggttttt cattattttt agtacatgca
     8821 gttataccta tagctaatac tagtgctaat cctacaaata aagttctttt taatttcatt
     8881 tttattaccc tcctagatgt tttatactat aaaaaatttt aataattttc tttcttaatt
     8941 tagtatttct atatttttat attttattta ctaaaattaa acaagttata atttattttt
     9001 ttggaaatca taatgaataa ataattaaat aaaaaaatta taattttatc attgatattg
     9061 attatcattc atagtataat ctaattactt gatattaatt ttcatttgaa ggaggttatc
     9121 ttttatgtct tgttgtaatt cacaattttg ttgtaaaaat aaaattgaat catcatatat
     9181 aaaatggctt ttagaagttt taggtccagt ctttttaggc tctaaacctg ctgaaataat
     9241 aaatatttcg tttactgatg taaatagaga ggaaaaaatt aatgatattt ataaatatct
     9301 atataagtgt aagaaaatag attttatagt tattgataaa gaaaaaaagg gattaaaaat
     9361 cttatttgta aataaaaaag cattatcaga aaaattaaaa tgtaaaaaaa cagtaaactt
     9421 tttaaagttt ttaggataca aacaaaatac gaatgttaat gcttatttag agcatttagt
     9481 agataaactt aagagtgatg tttttcctga tgaaataggt atttttttag gatatcctct
     9541 taaagatgtg attggattta tgggatatag taattatgaa gtttctatga ttaaatattg
     9601 gaaagtttat ggtgacacaa aacaatccga ggacacatat tcgaagtttt tacttcaccg
     9661 taagaaaatg agaaaattac tagactacat aagtgtagat aaaatagtaa gctgttttta
     9721 attgtgtttt tgtataataa taaaaataca ccctctttta ccatacagtt tccataataa
     9781 ataagggtgt attttccact ttaatatttt aaagtattct aattttgatt gtacaacata
     9841 tctttaatat ttattttctt ttgttgcatt ccattagaca tcaaaaattc ctgtgtatct
     9901 tctaattctt ttatatcctt gtccgttatt ttagaactaa agtcatacca agaatacatt
     9961 tcttttgttt catctaaaga taaaccaact tctttagata caacatccat cacttcatcc
    10021 ttgttttcat tcatatattt taaagtttct tcatgaacct tcataaatct ttttactaat
    10081 tcaggatatt tttcagcaaa gtcatcactt acagcagtaa ctattattcc acttactaat
    10141 ccttctccat ttacaatcat tttggcacct gaatttatcg ttttcaatgc cactggacct
    10201 gcaagcattg ctacatcagc acttttactt tctaatgcag aagcagcact aggaatatcc
    10261 atatttacat attcaacatc atctatagta tagccttctt tagcaagtgc agcaatcaac
    10321 agttgatgaa gtatagtccc ttttggacct acaatttttt taccttttaa gtcttttata
    10381 gatttaatat tatcattatt agttataagc atatatcctt ttggtgaacg gctataaata
    10441 ttcgtaattt taagaccaac accatttgat gcagctatta ttgcagacgt tcctcctaat
    10501 gcatgtaaaa agtctagctc acctgctgct aatgcttgag tttgctctgg tcctgttgtc
    10561 aattcatgga aattgacttt tatattgtcc tttttaaatt cctttccaaa taaatcatcc
    10621 tttttttcta atatactagg aacattaagt ggtgatttta cataagttaa atttatttcc
    10681 ttaggtaaac tagcctcttt ttttgcaggt tcatctttct tatcatttgt tgttgaacat
    10741 cctacagtcc caaccaacac cattattaaa gatgtcatta ctgctaaaat ctttttgttt
    10801 cttttaatca attaaaactc ccccaattcc cttaatattt cttttttcaa gtttatataa
    10861 tagtttttag ttaaatctct attatattca tcttcgacac taaattgttt aatcctttta
    10921 tttttactaa aaacaattat tttctttgct atttccatag cttcttcaat attgtgagtt
    10981 acaaatacta cacctttttt agtattttta tggatattta caacttcttt ttgcattttt
    11041 cttcttgtga agtaatctag tgctgcaaat ggttcgtcca tcagcaaaat atcgggattg
    11101 aaggaaagtg ctcttgctat acttactctg tgagccattc ctcctgataa ttcatttgga
    11161 tatgagtttt taaacttttc aagcttcatc atttttaaat acttgtctaa atcaacttta
    11221 tttctattat ctttttcagt atgtaaaagt atattttcac ttacattcag ccatggcata
    11281 agtctacttt cttgaaatac cattcctact tttggagtac actttttatc attattaaaa
    11341 aaatatacgt ttccactagt agcattttca agccctgcta aaattctaag tagcgttgtt
    11401 ttcccacatc cactttcacc taatataaca gttatctctt cactagatat atttaaagat
    11461 atattatcaa gaactaaatg ctctttatta tcaactaaat atttcttata tatattctct
    11521 agcaaaaatc cacttctaac catttaaatc aacctgcttc cctttgctaa attttgttat
    11581 tatctttgaa aatagataat ctgaaagtat acctaaaaat ccaataacaa aaattcctac
    11641 caatacaaca tcagttctag acatatcttt tccatcagaa atcaaatatc ctagtcctga
    11701 agaagctgca ataagctctg ctccaattat agccctaaaa ctatacccaa gccctaaact
    11761 aagtccaaca acaatatcca gcatagaatt agggattatt attttataaa aaattctctt
    11821 tttagataaa ttaaaagatt tacctacttc tattaattta ttgtcacaat tttttatacc
    11881 tttcaatgta tttaaaaata ttggaaaaaa ggatgccaaa ataataatta taattttaga
    11941 ttcttcacct ataccaaacc ataaaataag cattggcacc aaagctagtg gaggtgtact
    12001 tcgcataaat tcaaatagtg gtttgaaata ttcatacatt tttttatttg ctccaaataa
    12061 aactccaagt ggtatagcca tcaatgaact tattaaaaaa cctattaata tccttcttac
    12121 actggcataa acatttaaga aaatagaacc gtcatatatc atttttataa aagtatctaa
    12181 gactatttca ggaggaggca gaatatagct actccatata cccaaaacac atactatctt
    12241 ccataaaatt aaaattatta aaaaaatata atatccttta agccttttat gaattttctc
    12301 aaacatataa aactcctttt aacatatcta tccctgctac cctttttatt ttttaatatc
    12361 ttaaataata attaagctat atcataagat ttaatctatg ccaagtctta atccacctga
    12421 actcattcta tcagaaggtg tagaatctgt ttttaagcca gacaatcttt cagatggttg
    12481 tataatgact atttgtccag gttcaacaaa ttccatatga taagattctc ctgaagtctg
    12541 accaataaaa gtcttccatg atagttttga tactttagga tatggttctc ttccagtcca
    12601 agctactaat gcatctgggt caacacaaca aggtccttca agaattagag gattaccatc
    12661 tgttattata gcaacatctt gattttgacc tgttttattt actaaatgtg tagtaaaaag
    12721 acctttttga gatattaccc ctgaaccaat caattttaca ctgtaattta attgttcaga
    12781 aaatgccaat aagttttcac tctcaacata aatggaatca cctgggtcaa ttgatattac
    12841 atctacatgg taagcatttt gagctaggta aatttcacct tcaccttcaa ccaccattat
    12901 tggcatttgt tctcctgtca atgacctctg aacatgacct aataatgctc ttcccaatcc
    12961 accgccatta cttggaccta acagcaattt ttcaaaatta aaatttcctt taaaggctac
    13021 catagcacct ttttttgcaa aaaatttacc acttcccctt gctatacaca taagctcgtt
    13081 tgtaacatca aatttaaaca taatattcac ttatattgct taatttagca ataatccctc
    13141 ctaatcatat atttatatat aattatacag taaaattaca gcatagtcta acctacttca
    13201 aatatgaaat ttgttattta tacaaaaaat aagaacctga taataaagca atttaacttt
    13261 ttatcaggct cttatcctat ttatctctta gcgctatgac aactcttact acttgagcat
    13321 ccagaacaac cacatccaca actgctacca ttcttttttg aatttttaac catatacatt
    13381 acagctagtg ccattaatac tactacaata gctgctataa taaatgtagc catattcatc
    13441 actccttatt tatatgaatt aatttttcat ataacttaat ttacccattt tttctttttt
    13501 catattttta tttgaataag ttaaaactac aaagactact gctactgcta taaatataga
    13561 tattattgcc ccagcaatac ttcctccagt acctaaaact aaacttccaa cttgatttat
    13621 aagaagagca attatatacg ctgttaaagt ttggaatcct aatgtaatcc atgtccattt
    13681 ccatgaaccc atttctcttt ttatggctcc aatagctgca aaacaaggtg cacaaagcat
    13741 attaaatgcc ataaatgcaa acgcactagc tactgtaaac atacttgcaa ctgaagcaag
    13801 aagtgttggg tcttgctctg ttgcatctga tataccaaat aaaactccaa atgttccaac
    13861 tacattttcc ttagctacta gacctgtaac tgttgctact gatgattgcc aatttccaaa
    13921 tccaagagga gcaaatattg gtgcaactat atttcctaag ctagctaata tactatctcc
    13981 agcatctacc atttgtaatg accaattaaa tgattgtaag aaccagatta ctccacaagc
    14041 aacaaatata attgttcctg ctttgattat aaatgctttt cctctatccc acatatgtat
    14101 taaaacacct tttgcacttg gaatatgata ttgaggtaat tccataacaa atggagatgg
    14161 ttctcctgca aataaactag ttttctttaa tataattcca caaatgatta tcattgcaat
    14221 acctaagaaa tacattgatg gagctaccca tgaagcacct ccaaataatg cccctgcaaa
    14281 taatgctatt attggaatct tagctccaca tggaatgaat gtagttaaca taatagtcat
    14341 ttttctatct ctatcattct ctatagtacg agttgacata actcctggaa ctccacaacc
    14401 tgaacttata agcattggta taaatgattt tcctgaaaga ccaaacttac ggaaaattct
    14461 atccattata aaagctacac gtgacatgta cccacaatct tctaatattg ataataataa
    14521 gaataaaagc atgatttgtg gtacaaatcc tagtactgct ccaactccac caattagacc
    14581 atcaactaca agtccttgta accactctgc tacatttaaa gaagctagaa aattacttac
    14641 attgccttga ataatttctc caaacagtac atcatttgtc caatcagttg ctatagtacc
    14701 caatgaactt actgctatat agtaaactcc ccacattata agtgcaaaaa taggtaatgc
    14761 tagtatacga tttgttacaa tcttatctat cttatctgat acagtttcct taccttttcg
    14821 attcttcttt attatacttg aaattattga actaataaat tcataacgat tggctgttat
    14881 tatactttca ctatcatcat ccaattcatc ttcacaattt ctagttattt cttcaatact
    14941 attcaatatt gttttagaaa tatcaagttt ttgtataaca ttttcatctc tctcaaatag
    15001 ctttatagct aaccagcgtg tttctacatc tatatatgga gtcttttctt ctattatttt
    15061 ttcaattttt tctattgcat ccttactttc atcaacaaag ggtaatttga aatttaattt
    15121 attgttacta cttgcaagtt caactgcctt ttcagcagcc tccataattc cttgaccttt
    15181 aactgctgtt acttctacca ctgggcaacc tataacttca gatagttctt taatatttat
    15241 cttatcacca ttcttattta caatatccat catattaaga gctataacgg tcggtatacc
    15301 tagctctaaa acttgagttg taagatataa atttctctct atatttgatg catctacaat
    15361 atttattact gcatccggct tatcatctag cataaaatta cgtgtaacta cttcttctaa
    15421 tgtatatgga gataaagaat aaatacctgg taaatctact atttccacat ctttatttcc
    15481 ttttaatttt ccaccttttt tttcgactgt tactcctggc cagttaccaa catattgtga
    15541 tgagcctgta agcccattga acattgttgt cttaccacag ttaggatttc ctatgagacc
    15601 tatttttatt gacatttcca ttccctccat tattatttaa atacatcact gtttgtgata
    15661 cagaaaatca ttctcattat ataattacat taaccctact ctaattaatc tttattattc
    15721 cacaataatt ttttctgcat ctgacttacg aattgatagt tcataatctc tgactgttac
    15781 ctcaattggg tcaccaagag gagctacttt tcttataaat atattaacac ctcttgtaat
    15841 ccccatatcc attatacgtc ttcgtgtagc tccttctccc tctaactttt tgacttttac
    15901 agtctctcca cacttaatat ccttcagtct gttcatgtca ttacctccct atattattat
    15961 tctacttgcc atttttttat ctaatgcaac tctagtatct tttacgtcta ttattaagtt
    16021 accatctatc ttagaaacta ttttaacatt tgtacccact acaaaaccta tactatttaa
    16081 aaaagtcctc gtcttatcac ttcccgaaat tttctttata actacttctt cacctgtatt
    16141 tgataatatc aatggcatca tataatatcc ctcctcttaa agtaatttaa atctacaagc
    16201 aatcgttatc ttatatataa attacaatac cttaaatctt aatctattaa aataacatcc
    16261 tattttttcc ttctttttac aattcctaat tttgatttaa gtctatcaca atagtatatt
    16321 ttttgcaagt gattattgta ttcttttgat tttttttctc atttgattta ttttattaat
    16381 aaaatcgtaa aaattaatac aaaaataaag atgctctaaa atagtatact caactaatat
    16441 taaagcacct ttattatata tgtcattatt tacacattgc tttattcttt tttaaaagcg
    16501 attttttcaa actcttttac aggcattggc ttagaaaaca aatatccttg taccatatca
    16561 cacccaattt gctttagaaa ttctacttgc gaaatagtct ctacaccttc tgatattact
    16621 ttcattttca gttcctttgc cattttaatg acattagata taacaatttt ttcattatta
    16681 ctctcatctt tttctgtaaa aaatgctcta tctagtttta atatgtcaac aggcaattct
    16741 tttaaaagat taagagagga atatcctgta ccaaaatcgt ccatagagat tacaaatccc
    16801 ttttctttca aattgttcat tgtatcaagt aatccttcta tattatctaa tactgctgtt
    16861 tctgttagct ctaattctat aagtcttgtc ggaattttgt atttatctac aattaattta
    16921 aactcctcta taaaattact atttacaaaa tgcattcgag acaagtttac agatataggt
    16981 actacttttt gtccttcatc catccattct ctaatctttt tacacacttc ctcaaataca
    17041 tagaaatcaa tatttacaat aaaaccattc ctctcaaata caggaataaa ttctattggt
    17101 ggtattagac ctttttgtgg attatcccaa cgaacaagag cctctgcccc tataatttga
    17161 taatctgaaa ggctatattt aggttgaaaa taaacaataa attctccatt attaagagca
    17221 tctaccattg aattttcaat ttctttctct tttaatattt ttttgtgcat ttccttatcg
    17281 taaaaagcaa atgaattttt atgaccacct tttatagttt ttcttgcagt attagctcta
    17341 tccataatag tatttatgtc attatcactt ttacttattt tatatatacc acaatctaaa
    17401 atcaatttat aatagtttat tttaggatta ctaagtattt gaaccttatt gtaaatattg
    17461 tttaaccttg ttttaatatc atcatctgtc ttatacttaa taagaagaac aaaatggtca
    17521 gcagaaactc ttgcaaaaat ttcatcttcc tttaattcac ttgcaatagt atttgatata
    17581 tgtatcaaag tatcatttcc catatcatat ccaaacatat catttatata tttaaatttg
    17641 tcaacatcta tataaaacaa cgcatattct tctggattat tcttagcaaa cagtttatta
    17701 gaatttattt taaatttatc tatactatct gctcctgtca atgggtctgt atatgctatc
    17761 ttccttagat aatcctcttt cttcttttta ttataaatta tatatttaat agcaattaag
    17821 aataaaatac atacaaaaga aataatttgt attgaatatt ctttaaaaat cacaccaaat
    17881 ggtactttat aaggtttgtc aatagtatta gaatatatac aatcacttaa ttcttcttca
    17941 gtaattttgt ctattgattt atttaaaata gacactaata tagggtcagt tttatttgat
    18001 acccctatac gcatcccata gcttgtatct aaaacagata taacagataa attctcactc
    18061 tttccttctc ttaaaagttc atcaaactga taagaactaa gtatcattaa gtttgcttta
    18121 ccttcattta tagcgtttag acaactttct acactcttat aagatgttgt cttagcattt
    18181 tcaaaactct tttctatata agaatcaata gacttataac tactagttaa agcaatattt
    18241 aaatcattat tgagattggt atccaatttt cctaccataa tcattggaat atttatgtat
    18301 ggatttgtca atttcatatt aaatcgtttt accttatcat tttcattgat tgagccacat
    18361 agcatagttg ccttaccact ttttatgtaa tcaagactct cattataatt ttttgtttta
    18421 atatattcaa attttatacc acattttttt gatataattg acattaaatc ggaacttata
    18481 cctttaaatg aattgctttc ttcatcataa tactctacag gagaccactc tgaatcatat
    18541 accatagtaa gttttgggtt tgctttaata aaattcattt cttgccgagt aaatgctatg
    18601 gaattattga cctctctttt gaaatatttt ttatataatt ccaactcata atacatatca
    18661 tttattttta attcttttaa agcataatct aattctttta ctaaattagg tttatctttt
    18721 gcagtagcaa aataaaaagg ttctactgaa aatttgctaa ctattttcat tcctttagca
    18781 tttataatac ttccacaaac aaatgcatct gccttttttt cattgacgga ctcaattaat
    18841 tcatcaatag aattacagta tatatattca actgaaaaat tattttcttc tctatacttc
    18901 tcaaatgcag tattatgaaa gtttcctcta agtacagcta ctctcattcc cttaaaggaa
    18961 cttatatctt cataaaacat attattgtta ctatcctttg tatatagtgc actatattca
    19021 tatcctgaac ttagtttagg aaaattaaat agtttatttc tttcatcatt cttctgtaaa
    19081 ggtccgagta agtctatttc acccttttct agtctgtcta aacattcttc ccaagaccct
    19141 tttacaaatt catattccca tcttgtatat tttgagattt ctcttagata atcataacca
    19201 tatcctgtat aagaatgctc ttttgatgag gatattattc cgtagtcatc acaatatcca
    19261 actcttattt tttttgaatt ttcagactca tcagcaaagc atagtatatt gttaaaacat
    19321 gatataataa gtaaaaatat caaagaaagt atcaagaagt gctttttaaa tgctttcata
    19381 tttaaaaacc tccaatttgc caacttactt taacttattt cgttttctag ttgtaactac
    19441 ttttattgta atggtaaata tatcaaattg caatattctc atataatatt cttattcaac
    19501 tataatgaat ttattattat ctatttaaat acactatcta atattagttt cttataagtc
    19561 ataaaagcac aactaacact ataattctag tgattaattt tatttcaaaa aatataataa
    19621 cactactaat attttaaata tcaatagtgt cataaaaact tttcagatta attaaatctt
    19681 tctatatatt ttataataat ctaaatcatg ctaaatattt agacagaaaa cttttttctt
    19741 taatagtaaa tacttttgtt tgaatatcag aataattatt tgaatatcta tctataaaac
    19801 catgatttgc atctaaaatc tcaatttgga tattttgttt cctagaaagt tggtaagaaa
    19861 tagaatcaac atcaaatgaa tcgtgctttg caaatactaa taaggtagga cattttggaa
    19921 ttacatccat gtaatctcta atccgagagc cataacaaca aattatgcca tcacaaaaag
    19981 aatcttcact gcatctccat gcaatagttg ctcccacgct atacccagca acaaatacat
    20041 atttatactc tgattttaaa tcatttataa gcttttcaac ttttttataa atatcaaaac
    20101 caacattatt tataaaaaaa tcatatgcct cttggacttt tgaatatgga aaaactcttt
    20161 tttctcccag aagttcagga caaaatacat catatccata tttataatac ttttgacaaa
    20221 catcttctat aaagctatta atcccataga tttcatgcaa aacaacaata gctacatcac
    20281 ttttatgtat atactttatc agattatcca ccacctttac aaaacagcaa attttttata
    20341 cttagtttaa tatattcttc tttacatgtc aatttacttt tctaaaatca aatttatttt
    20401 tggattaatc atttattttt aaaagttgtg cattattact attaaattat tctctattat
    20461 taaaaatata atcgttagaa ataaaaaata aagtacaaat aattaaaaaa tatctcactc
    20521 ataactttaa aaattataag tgagatactc ctaaaatcta ctttatatta aatttatttt
    20581 ccaaaatatc tatttaataa tcctaaaaat gctttaccat gtctagcttc atctttacac
    20641 atttcatgta ctgtatcatg tatagcatct aatcctaatt cttttgcttt cttagctaat
    20701 tttaattttc catcagttgc accatactca gcatcaactc taactcttaa gttttctttt
    20761 gtatctgcaa ctacaacttc tccaagaagt tctgcaaatt tagcagcatg ttcagcttct
    20821 tcaagagcta ttctcttata agcttctcct acttctggat aaccttctct atcagcttgt
    20881 ctactcattg ctaaatacat tcctacttct gtacactcac caacaaagtt agctcttaat
    20941 ccttcgatta tttcttcatc tacaccttga gctactccta ttctgtgctc atcagcccaa
    21001 tttttttcgc ctttcatttc ttcaaattta tcagctccaa ctttacatac tggacattgt
    21061 gctggtgcag catctccttc atgtatatat ccacatactg tacaaacaaa tttcttcata
    21121 attactttcc tcctaaattt tatatatcaa attaaatttt ttatttatta agttagtcta
    21181 tatatacccg caccatatac ctttaaacaa tttttttaaa agttttttct aaatacttat
    21241 tttccctgtg tcaataatat gtttctataa taatgtccct ttaattttat caattcttta
    21301 tgatttcctg attccacaat tctaccattt tctaaaacta ttatattatc acaatttgat
    21361 atggtactta atctatgagc tataattata cttgtctttc cataagttaa attatttatt
    21421 gcttcttgaa ttttagcttc tgttgctata tctatgttac ttgtagcctc atccaaaata
    21481 agtattggag cattttttaa tactgctcta gcaatactta ttaattgttt ttgaccttca
    21541 cttaaatctt cacccctatc cgataataca gtactatatc cttcacgcat tttaactata
    21601 aatttatgtg catttgctat tttactagca tatataactt cttcatcagt agcctccaga
    21661 tttccatacc ttatattttc cataactgta tcattgaata tataaccttc ttgtggaact
    21721 actccaaaac aagttctcaa atagtttctg ttaatagagt atatatcctt accatctaaa
    21781 agtatacttc cttcatctat atgataaaaa tttgtaatta gatttataat agttgttttt
    21841 ccacttcccg tctctcctat gatggcattt gatgtgccag ctttgatttt taggtttata
    21901 ttatctaaaa ttttcttttt tgaatcataa gaaaaactta catctttaag ctcaatttct
    21961 ccctttacaa aatgagagtt taaattacca tctttattat cgttaatctc acttttaaac
    22021 atgttatgca cttcttgctc ttcttgttca tctataattt caaaaactct ttcacatcca
    22081 gctatggctg aaagtaatgt attaaataga tttgctaatt catttaaagg tcttgtaaat
    22141 tgtcttacat aacttaaaaa actagctata acccctatgg ttataatatc tttagatgca
    22201 agaatgcctc ctataataca tatagttgca aatcctatat tactaatcac attcataata
    22261 ggcattaaaa gagaagtgta aatttgcgat ttaagggaaa tatttagaat tctatcattg
    22321 acttctttaa aatcatttac agattttctt tcataattaa aagattttac tacatttatg
    22381 tttgatatca tttcttcaat atgtgcattt agttttccca actcaacttg ttgttgctta
    22441 aaaaaaactc tagttctctt tgcaataaaa ttactcacaa acattactat cggtattaat
    22501 attacactgc aaaaggttaa aattgggctt aatacaaaca tcataataaa agtcatcaat
    22561 atgtttaaga ttcctgtaat taactgtact aatgaatttg atattcctgt actgatatta
    22621 tcaacatcat ttgtcactct actcataata tctccagtag aacttttatc aaaaaaggtt
    22681 attggtagat ttttaaattt taaaaataaa ttacttctca tttctttaac tatactttga
    22741 gataatctag ctgtacatat ccccttagac agtttaactg aaaactcaat tatataaaat
    22801 gaaagtaaaa taattattcc ttttaagaaa atggattttg aataagtatc gatatctatt
    22861 atgtcaacta cctttccaat agtatatggt attacagttg atataattga atcaattatt
    22921 actagcaaaa aaataaatct agtaatcttc ttatcttttt taaagtacat aaaaattctt
    22981 ttaaacgttt ttttaaaatc tttaggcttt tcttttttct ttaatctact gaccttctga
    23041 tttccagtac ttaaattcaa tatacttcct ccttaattta catcttcact tactaaattc
    23101 tgcaacgcaa aaattttttt ataaattgga gaactaacca gtaaatcttc atgtgtagca
    23161 aatccaacca cctcaccctc atccaagact aaaatcttat cacagtcaat tatagaacta
    23221 atcttttgtg aaatagttat tacagtacat ttcttacttt ccttttctct taaattactt
    23281 atatactctt ttatattctt tcttataatt gtctctgtat taatatccat tgcactaaaa
    23341 caatcatcaa gaattaatat ttgtggtttt ttggctatag ctctagctat agtaattctt
    23401 tgtctctgtc ctcctgataa atttttaccc ctttggaata aaacttcatc ataagtattt
    23461 tcgagttttg ttataaattg atttgcattt gctatttcac atgattttac aacatcctct
    23521 ttgcatatat ctctatttcc cataactaca ttctccaata tactttttcc aaacacacta
    23581 ctattttgaa atactacacc tattcttgcc cttaaacttt cttcagaaat actgtatata
    23641 ctacgtcctc caatcttaat atcaccttta tcaattatat ataatttttg aattaaattt
    23701 actaaagaag tctttcctga accagtttga ccaatcacac caatattttg accttcttct
    23761 attttaaagg atacgttttt taatattttt tcatctgtat ttttgttata cgaaaaatct
    23821 acattttcaa aaattatatc acagtttaca aactttaaat ctttttctcc aacatctaaa
    23881 ctttcttctc tctcaattac ttcttcattc tcatcaacca aaacctcact tattctctca
    23941 taagatgctt ttgccctaac aaaaacatta aaaacatgtg aaatcattat aagagatgtt
    24001 aatattctag tcatatagtt tacataagca actataatcc caattttaat ttctcctgaa
    24061 taaattaaat gccttccaat tagtaatatt attattatgg aaacattaat gattattgta
    24121 attgtcggtc taaaaaatga catcatagtt gtagcttttc tactattttt aaaaagttca
    24181 tcatttaact cttcaaagct ttggttctca taatcgtatc tacaaaatag tttaactact
    24241 tttattccac ttaaaaactg tcttaatttt gaatttactt tatctgtact ttcttgtaca
    24301 tttttaaata atctataccc tatcttcata ttaagcaata ttataataaa tataattaaa
    24361 actgcaatta gaataattat tgacatcttt aaatttaatt ttatagctat aattaaactt
    24421 cctatacaca ttattggtga ctttataaaa actctcatca ttccattaac gaattgttgc
    24481 atttgattga tatcattagt aattcttgtt attaatgaag ctctaccaaa tttatctatt
    24541 tgttttaatg agtatgtatt tatcttttca aatacatcct gtcttaaatc ataagaaaaa
    24601 tgctgagaaa ctcttgccga gattacactt cttattattg caaatattgc acctaaaaaa
    24661 gttataccta tcattatagc tcctgttgat agtatgtagt ctaagttctt atttgaaacc
    24721 cctatatcta ttatttttgc cattatagtt ggttgagcta aatcacaaaa agcttccaat
    24781 gttaaaaaaa atactgctgt tagaaataat actatatatt tttttatgta ttttttaaaa
    24841 tacctcattc aatcatccct tcatagaatt ttctcattac caaaagtata tcataatggc
    24901 taatttaatt gattatatca attataaata tttgatttat tattaatttt tataccatat
    24961 attttataaa ttatttgttt ttatttccta tat
//
LOCUS       DAYAOX010000036        24922 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.036, whole genome shotgun sequence.
ACCESSION   DAYAOX010000036 DAYAOX010000000
VERSION     DAYAOX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.036"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(72..1871)
                     /locus_tag="ACGI57_001180"
     CDS             complement(72..1871)
                     /locus_tag="ACGI57_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HFG9716265.1"
                     /translation="MYAPFFIRFLLSTLTLSILIAVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(1874..2260)
                     /locus_tag="ACGI57_001181"
     CDS             complement(1874..2260)
                     /locus_tag="ACGI57_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9716266.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            2493..3170
                     /locus_tag="ACGI57_001182"
     CDS             2493..3170
                     /locus_tag="ACGI57_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9716267.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            3146..3607
                     /locus_tag="ACGI57_001183"
     CDS             3146..3607
                     /locus_tag="ACGI57_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9716268.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRVLDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            3675..4454
                     /locus_tag="ACGI57_001184"
     CDS             3675..4454
                     /locus_tag="ACGI57_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFG9716269.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(4701..5327)
                     /locus_tag="ACGI57_001185"
     CDS             complement(4701..5327)
                     /locus_tag="ACGI57_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFG9716270.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(5470..5727)
                     /locus_tag="ACGI57_001186"
     CDS             complement(5470..5727)
                     /locus_tag="ACGI57_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716271.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            6053..6565
                     /locus_tag="ACGI57_001187"
     CDS             6053..6565
                     /locus_tag="ACGI57_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716272.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSDCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            6820..7524
                     /locus_tag="ACGI57_001188"
     CDS             6820..7524
                     /locus_tag="ACGI57_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HFG9716273.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            7521..8291
                     /locus_tag="ACGI57_001189"
     CDS             7521..8291
                     /locus_tag="ACGI57_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HFG9716274.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            8288..9067
                     /locus_tag="ACGI57_001190"
     CDS             8288..9067
                     /locus_tag="ACGI57_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HFG9716275.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            9119..9811
                     /locus_tag="ACGI57_001191"
     CDS             9119..9811
                     /locus_tag="ACGI57_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9716276.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            9830..11254
                     /locus_tag="ACGI57_001192"
     CDS             9830..11254
                     /locus_tag="ACGI57_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFG9716277.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(11494..13962)
                     /locus_tag="ACGI57_001193"
     CDS             complement(11494..13962)
                     /locus_tag="ACGI57_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9716278.1"
                     /translation="MKNYLSLSIKELKSQKFMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRYTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNRSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(13965..14633)
                     /locus_tag="ACGI57_001194"
     CDS             complement(13965..14633)
                     /locus_tag="ACGI57_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716279.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            complement(14919..15446)
                     /locus_tag="ACGI57_001195"
     CDS             complement(14919..15446)
                     /locus_tag="ACGI57_001195"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFG9716280.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFSSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            complement(15662..16822)
                     /locus_tag="ACGI57_001196"
     CDS             complement(15662..16822)
                     /locus_tag="ACGI57_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9716281.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            complement(16810..17484)
                     /locus_tag="ACGI57_001197"
     CDS             complement(16810..17484)
                     /locus_tag="ACGI57_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9716282.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            complement(18306..19133)
                     /locus_tag="ACGI57_001198"
     CDS             complement(18306..19133)
                     /locus_tag="ACGI57_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFG9716283.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     regulatory      19339..19445
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            19515..19835
                     /gene="sugE"
                     /locus_tag="ACGI57_001199"
     CDS             19515..19835
                     /gene="sugE"
                     /locus_tag="ACGI57_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFG9716284.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            complement(19968..20648)
                     /locus_tag="ACGI57_001200"
     CDS             complement(19968..20648)
                     /locus_tag="ACGI57_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFG9716285.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            21044..22096
                     /locus_tag="ACGI57_001201"
     CDS             21044..22096
                     /locus_tag="ACGI57_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFG9716286.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            22143..22559
                     /locus_tag="ACGI57_001202"
     CDS             22143..22559
                     /locus_tag="ACGI57_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9716287.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIVINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            22588..23061
                     /locus_tag="ACGI57_001203"
     CDS             22588..23061
                     /locus_tag="ACGI57_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFG9716288.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            23108..23866
                     /locus_tag="ACGI57_001204"
     CDS             23108..23866
                     /locus_tag="ACGI57_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9716289.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            23890..24729
                     /locus_tag="ACGI57_001205"
     CDS             23890..24729
                     /locus_tag="ACGI57_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9716290.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
BASE COUNT         8827 a         3146 c         4035 g         8914 t
ORIGIN      
        1 ccttgtatat gaatttaatt gataattaat atgaaaatta tgtgaatttt ttagaatgta
       61 cagtaaatat attaaggatt atagattttt ttgtcattta atatggataa tgttatctta
      121 gatgctgaac taccattaga ataatctttg ctttgaatat ttgtagcaaa gaaataagta
      181 ttttcatttt tttccacata acctataaac caaccattta catcttttcc atttatattg
      241 gcagtgcctg ttttaccaaa aagtgatacc ccatcctttt ttgatattaa caatgaatct
      301 tttactgttt ctatattttt atcttcaaat ttaaacttat ttgtataaaa actttcaagt
      361 aattctacct gttcaatagg tgatattttt aatgaagatt ctagccaaaa gtttgagata
      421 ccacctgaaa catcataatt gccatatcca atttgcctaa gataagtttg tatgtttttt
      481 agccctgttt ttttatctag agtctgaaag taccagttta cggagttttc catagcagaa
      541 aataagtctt ggtcttcatt ccaagaacta ttagaatgat gctttccatc ccaagggata
      601 tgtgaattat ttcttgttat tatcccattt tccaatccca ataaggcact ataaatctta
      661 taagtagaat ttggagaaat tcttgtttta cttttttctt tattgtaaat atgatattgc
      721 ttggatttta aatcatacat aacaaaactt ccatcaaaac ctttaaaata ctgacttaag
      781 tcctcgtaca cagtttgttc atttgtaggt ctatattttt cattgatatc tgccataata
      841 gatagtttgg gaatatttcc aaatacaatt aaagctatca atgtaaaaat taaaatgctt
      901 ttgaatttta acgtatttga ttcattatga aaatccatta tcttcaatag tcgctttttt
      961 atttgttttt ttgacccacc aatatctgat gtaagtgtaa gatttgatgg tcttgaaata
     1021 ttatccacaa agtttattag agtttgtcca tattctatat gactatcttt atcaagcttt
     1081 tttaaaactg aaatatcaca tgcaatttca cggtctatac gcatttcttt aaatgcatac
     1141 catacaagag gattaaacca atataatatt tgaaggaagc acattatata atttatcagt
     1201 atatctttat ttttaaagtg ttgtaattca tgaagtaaga tatattttat atcttttaaa
     1261 gataatttag atatagactt atctggtaat ataatgtgtg gtttaaagta tccaaatgta
     1321 ataggtgtat ttataatagg tgatttgcat agtataaaat ccttatttat accaatatct
     1381 tttttacact tttcaaatat aaatactatc tcctcatctc ttaaaatttg tattgatttt
     1441 ttcatatttt ttattttaat gttgcaatga atagtaatag ctatcatgat taaaattcca
     1501 attatccatg atattagtaa taaaatattt aaactatcaa aattaacttg gtttagggat
     1561 actgaaaaat cgtttaatgc atttgagtta ttaattatag aatttggact attttttatc
     1621 atagaagtcc tattagtact gcttccaagt tgattaaaag tatataaata gttaaataca
     1681 tttccaagat taatatattt aagagggaca aatggaatta ttaacattat aagcaatata
     1741 aatccaatat tgtattggta ctttgcagaa atatgttttt taaatacttt tttggcaagt
     1801 aaaattacag caattaatat tgataaggtt aatgtactga gtaaaaatcg tataaagaat
     1861 ggggcataca tatttattta tcccccttag tcgtcctttt atttaagatt tctctcagtt
     1921 cctcaatatc atcttcagat agtttatcat tttctaaaaa gtttagtacc atagaattaa
     1981 gagtaccatt ataaaaacga tttaaaaaag aactactttc tgtagctacg tattcttctt
     2041 ctttaacaag tggagtataa acgaatactc tgctattttt ttcatatgtt aaagctcctt
     2101 ttttaactaa tctcaataac attgtttgaa ttgttttagg actccatgta cttgtttcta
     2161 ccaacttttc aattacttca gtagtgctga taggagcata tttccaaata attttcataa
     2221 cttgatattc tgcttctgaa atatgtggta actttttcat actatacctc cttctactta
     2281 catttgtaat acataaagta tattatcatt aatttagttt gtcaattatt aaaaataatt
     2341 catttatagt tgattaaata agcacatgta gttaaataaa acttttattt aagctgtgta
     2401 agtatagctt taaataaatg gtttatattg acagctaaaa ttatagttga tatacttatt
     2461 gttgatataa caactaaaaa ggagatatag aaatgataat tacagttata aatggaagcc
     2521 ctagaaaaaa tggagctaca tcaaaagttt taacttactt gtataaagat atagaaagat
     2581 taataccaga tgtaaaaatt aattattttg atttaagtga ggtaaatcca tcatattgta
     2641 ttggttgttt aaattgttat aaaatgggca agtgtatcaa tcaaaatgat aaagtagagt
     2701 atattcatga tataattact aagagtgatg gtgttatatt tggtagccca acttatggaa
     2761 gtagtgtaac tggacttttc aaggtgttta cagatagagc acatatgatg cttgaaagat
     2821 tattgtatcg taagccatgt atagcagtta ctacatatga aaatgcacgt ggttcaaaag
     2881 caatttcttt tataaaatcc atggtcttag attcgggagg ttatgtttgt ggtagtttat
     2941 ctataaaaac tggatttaat caaaatccta taactgaaaa agtagaatca aagatacaaa
     3001 aggtatctaa aaaatttatt tattgcatag aggaaaagaa aaatccacct gtgctttctc
     3061 aaatctataa ttttattgct ataaatgctg ttttaaagcc aatggctttt aaagatattg
     3121 agcaatataa gggaataatt gatagatggg aggagcaagg gattatttaa atggaggtaa
     3181 aatgaaagat tttgagaaac tatatgaagc tacaactaag ttagtaagta cttcgttaaa
     3241 agtaaaagat gatttaaaaa agatgtttaa acaaaatgga tatgatataa caactgacca
     3301 ttatgcgtta ttaaggtttc tatgggagca agatgggatt tctcagatag atttatgtga
     3361 aaaatcatgt aaggataaat ctaatacaac aagagtttta gatgttatga aaaataaggg
     3421 attaattgtc cgtaaagtag atgttaaaga tagacgtaag tttcaaatat tcctaacaga
     3481 tttaggtaga gaattagaag aaccattaaa tgaaattgca agtatatatg caacagagac
     3541 atttaaatct atctctgatg aagaattgcc tttgtttact gatattttgg atagaattgg
     3601 aaagtaaata taaatcctat tttaagagac tttttaacta ttgatgatgt agaaatatag
     3661 aaagagggat aagaatgaat agtaagattt attatttttc tggaacagga aattcgctag
     3721 tagtttcaaa tattttaaaa gaaacgttaa atggaagtaa agaagttata cccttagcta
     3781 tacatagaaa agaaaataat atagacataa atgaagatat attggtaatt gtttttcctg
     3841 tttattttgc tgatgttcct gatatagtaa agtcttttgt agagaaatta aattttaata
     3901 ttaatactca tatatatgcc attgccactt gtaatggggt agctggacat agtttattta
     3961 ctatagataa gttacttaaa aaaaggggga aaaaattatc tgctggtttt ttaatcaata
     4021 tgcctggaaa tgctttgata acacctccag atgttgaaga agaaagattg aaaaatcaga
     4081 ctaatagggt agctgagatt gcaaaatgta tcaataactt ggagattgga aatatagaag
     4141 gaaaaaacaa tttaaagtca catatagata gtgttgtact tcgtttagta ggtaagaata
     4201 tgcaaattgc aactactaaa ttttttgcag aaaataaatg taatggatgt gggacatgta
     4261 aaagggtttg tcctgtagag aatataagta tagttgataa aaagccaaag tggggtaatg
     4321 agtgtgaaag atgcctagct tgttttcact ggtgtccaaa agaggctatt aatgtaaaaa
     4381 aatctttttt aacgaatagg aagaagtatc accatccaga tattactgta aaagatatat
     4441 ttttagataa atagatatat ttgacttgta ttaataattg ttttattaaa taaaaacgtt
     4501 agagatataa aagaaatata aagtaatctt gtaaagagta atgttaccta gactaaatgg
     4561 aatagagatt aaaaagaaca atataaataa taaattatgt taatagtata tagttacact
     4621 aatataaaat aaaagtgcct cataaataga agtttttgag gcacttttta ttttatatta
     4681 gtttttatta ttttgtaata tcaaccgttt ataatctcga acattttttt tatatcttct
     4741 gatgccattt caaattttga tttttcaatc agtatgtttc caccagtcat aatatatgat
     4801 ggaaatactc ttaaattatc ataataaagt tcattattac gaagtttata tgttgaaatg
     4861 gaagggatga cattcttttt tatatgcttt ttagtaatca tattaaaaga tttttttgca
     4921 acatctccca ttaacattac tacctctagg tttttaaata atttaatttc ttcttctaaa
     4981 ataggcatat gtaatttaat tgtatcacga gttattgtat attctgattt aggaagtttt
     5041 actgcattag ttatatagat acccatatct aggatatcat ttatgttaga tacttttatt
     5101 cctgcactgt caaataaagg aatagtagtt tttaaataat cagcatcttt atctacacta
     5161 taaaaatcgt cattggtgtt agttgggaca acctcattaa tcattatagt ttttattttg
     5221 tctgtttcta ttgttatatt aggaagatag gatattattc tgctgtcatc aaaaagattg
     5281 ttctgtttaa taaattcact taactttgat tttatattta ttttcatata acgagtctcc
     5341 ttagttttaa gctttaaatt actaaaaaaa gctagtttta aagcacacaa ttttgaaaat
     5401 atagtagcat atttgaaagt ataataacat ataaaacatg acaacagaat gtcatgtttt
     5461 atagataatc tatcgcaact tagaatttat tagagactct gcctgttcat aatcgtcttt
     5521 atgaacataa attaagtact cataagagaa atcttccttt tggcctaaat tgccaagtgt
     5581 tatttcatta aaaaaaggtt ttttagagta agcattattt ttcactttat acttatattt
     5641 aattttattt actgataata tattgcatat ctcgttaaat ctatttaaag aatatcctcg
     5701 gaaaagctca gctctattta aaatcataat gaaaacctcc ttttaaaagt aatatattga
     5761 aaagcatagg agaaatgtac tataagttgt tataaataaa aggtagcaaa tttaaattga
     5821 tttgtaaagt ttttaagagt attatattag tgataaaata aataattttt agaatataac
     5881 taataacaat ttaaaataag taacttatgt aagaatcgta ggaactacgg agatatcctg
     5941 tagagaatta ataagacata tttaagtatg caaaattcta ttaagtagaa accttgtaac
     6001 tttagtcatg tgagattcat aaggatattt aagtattgaa aggagagttt aattgaaaaa
     6061 aatatctcaa gaaaaaataa gtacacatat ccaagaaata ccacaggaaa aaattgaaca
     6121 gtggaaatct ttatatgaaa aatataaaag tattttaaaa gtaaattcta agtctgtttt
     6181 agaaataata gagtttttaa agcgaaaata tgtaatagta gaagaaactt cagaggaatt
     6241 taaacatgta gttatcaata atattaagtt aaacaagcct ttttcacaaa agatttccag
     6301 tgataaagaa ttaaaaccta ttgtatttac aattccaaat gaaggaaatg caaaaaaatt
     6361 gtacgatact caagaggaaa tttatagtga ctgtaagatt attgtaggta tggaatttga
     6421 aacaggttgt gtttttgtag aaggtagtga tgagcttaat gatgaaataa cttcttttaa
     6481 aggattagat aaatatgata taaataatta ttatttagta gcaaactata ttagatgtct
     6541 aaagaaatat aaattaatag attagtgttt gattaataaa ttagtgtttg atttaaagat
     6601 tatagagtaa atttgaagtt agtgtgataa tgaaactaag tttaaatcta atataactgt
     6661 aaaacataaa cttaaataga gttgccaaaa taaaattggt aactctattt tttattttaa
     6721 attttattaa aattagtagg tgttatagaa ttgttatttt ttcatgttag tattctctta
     6781 gtaacagatt taaggtatta caagaaggga gaaattaata tgaaagattt aattcttgaa
     6841 acaaaaaact tatcaaaaag atatggagaa caaatggcag ttaacaatgt ttctctacaa
     6901 attgaacgaa atagtatata tggactttta ggaccaaatg gagcaggaaa gtccacaaca
     6961 cttaaaatgc ttgttggtct tcttcgccca acaaatggtc gtattatttt tgatagaaag
     7021 ccttggaaac gagaatctct tgcaaaaata ggttctttaa tagaatcccc tgcattgtat
     7081 ggaaacctta cagcagagga aaatttgtta gtacatacaa aaatattggg aatacctaaa
     7141 gataaaattt atgaagtgtt ggaaatagta aatttgaaaa atacaggtaa aaagagagct
     7201 tcacaatttt ctatgggtat gaaacaaagg ttaggaatag ctattgcact tttgagtgac
     7261 ccagagttgt taatactaga tgaaccaacc aatggtcttg acccctttgg cattcaagaa
     7321 ttgcgtgaat tgattgcttc ttttcccaaa aaaggtataa ctgtaattct ttctagccat
     7381 attctatcag aggttgctca agttgtagat aatataggaa ttataaatgg aggacgacta
     7441 ttatttcaag gaatgcctga ccctaatgaa aatctagaag aattttttac agatgttatt
     7501 cttaaaggag gacaaaacag atgaatgctt tacaatcaga acttttaaag tataaaagaa
     7561 catttatggg taaacttatt gtttttttcc cagtgtcttt tgcagcatat gcatttataa
     7621 tgcaatcaac actcatgcaa aatccattat ctcaaacaac ttcttgggcg tggcaaaatc
     7681 ttttagcatt aatttttaac tggtggtcat ttttatttct gcctattgga tttgcattgt
     7741 tcgctacttt ggtagctttc caggaaaaaa aagctggaaa ttatcgtgct ttacgtacac
     7801 acaatgtgtc tcctatgaca ctttggatta ataaagttat agctatggca gtctacagtt
     7861 ttatttcaac gctagtttta atagtcgtaa caattataac tggtttaata ttgaaagctg
     7921 gtccagttcc ttttggacag attatagggg caagtattgt atgctgggtt gtttctcttg
     7981 ctatacttcc acttcagcta tggctagcta catggaaagg tatgttccta agtatgggag
     8041 ttggagcttt aggtatgata tttggtgttc ttgctgcgac aaaaccattt tggattgcag
     8101 taccttggag ttgggcagtt agaatggtat gcccaatcat caacatacat cctaacggta
     8161 ctatattaga agctggagac ccacttttga atacttcagt gattcctatt gggattgttg
     8221 tttctttggt tgtgtttata gtattaacag cacttactgc agcttggttc aataggaggg
     8281 atgacaaatg ataaaactat tatcatcaga atggcttcga gcaaagagga cagctgtcca
     8341 gtggcttact ctttgtatgc ctattatttt ttcattatgt gtggtttttt atctcacaac
     8401 taaatctgga agtacacaag aatttgcttt tgaaggattt tttacagtat ggactgtatt
     8461 catcattcct attggagtag gtattttagc aggatttata gtgcaggagg aagagcttgc
     8521 tggaaatttt aatggttttt tatgtgtagg tatttctcgt gtcagattat atctgggaaa
     8581 atttcttttt cttctgtttt gcctaactat atgcacattt attgctacgt tgatactttg
     8641 cataggaatg agtattgcag tgccatctgg agcaacaata tggttattcc tgtcagcagc
     8701 aggacttgtg gtgattggga cacttccact attagctatt catctttggg tgagttttgc
     8761 atggggtatg ggtgcatcta ttggtattag tattggtgga attttgatgg cagcaatact
     8821 tggattaact agcgttggtg aaaaagtatg ggcatttgtt ccatggacat ggccagtaaa
     8881 actaggtatg cttccaggaa tttattttat taaagaagct ggaactattt caactgaggc
     8941 attttactct aaagtgatgc aaacagctag tatagagctt atagtagtag caataggttt
     9001 gattatattc ttgataggag gcgttatttg gtttaaaatg tgggagggaa ggaaaagtta
     9061 tgagtagacg aatattgttt taatactgac cttcccataa gagaaaggtg gtgttatatt
     9121 gagtaaaata ttgattattg atgatgaaat ggacctagta atgctacttg aggatgaatt
     9181 gaaggcaaaa ggacatgaag tgttgatagc ttatgatggt caggcaggta tagagttatc
     9241 aaaacaggag cctgacctta ttatactgga tattatgatg cctaaaatga atggatttga
     9301 ggtatgtcag gcaattagag atgatgtttt atgcccaatt atatttttaa gtgcaaagca
     9361 atcagaaacc gacaaaatca aagggttgac ctttggaggg gatgattatg taacaaagcc
     9421 atttggactg cgtgaactta tggcaaggat tgaagctaat ctgcgtaggg aaaagcgttc
     9481 acagtatatc aatgaagaga acaagagatc tactttgtat tttgggaagt tatgcttata
     9541 tatgaaggaa cgtgcaatta aaattgatgg taagaatatt gatttaacaa aaatagagta
     9601 tgatattgtt gaattactag cactccatgc aggacaagtt ttctcaagag aacaaatcta
     9661 tgaaaaagta tgggggtgtg attctgaagg tgattcctca actgttgttg aacgagtaaa
     9721 aaaaatacga gctaagtttt ctgctgtgac accagaaaaa gaatatattt ccacagtttg
     9781 gggtattggg tataagtgga ataaaatgta aagagagaga ggttattaaa tgaaaaatca
     9841 atcccttatt acccaattta gacatacttt tatttttatt ataatagcca gtatagttgc
     9901 cacagtgatt acttatgtat ttgctctcta tttgtatatt cattccttaa ataaggatat
     9961 ctatccacca aactattatg agcgacaagt tcctagaatt gaaaaataca tcaatgaaaa
    10021 aaacatagct ttgttatcac aatcgaatga agaaggttta aaaagaacga ttcgtgggga
    10081 tgatatgcta tatcaagttg tggataataa tggcaatatt ttatatggta ctaaccccaa
    10141 aaaactattt aaaacaaaag aagaattatt taataatttt atcaataaaa ctgttcgtaa
    10201 aggtggttat atccatactg ttccaatcaa aggggataat ggaaagattg agggtgctgt
    10261 tattctattc taccaagtga aaatcacctt tgcaaatatt agagggagat ttgtatttgc
    10321 agttattata atggctttgt tttctccatt tctatatatc gtgggtttta ctagatggct
    10381 ttctaagcgc tttgtaaaga atatcaatca accattacat ctattgattg atgcgtccaa
    10441 gaagataaaa gaaaaggacc ttgattttga aatcgactat tattcagata atgaactcgg
    10501 taaactttgt agtgcctttt ctgaaatgaa agacgaatta aaaggctctt tatctgcaca
    10561 gtggaagatg gagcaggaac gtgttgaaat ggtggaagct ctagcacatg atttaaaatc
    10621 tcctctttct attattttgg gatatacaga tgcacttatt ggtaataata cagatgacaa
    10681 tgaaaaactt cataggtatc ttacagtgat aagagaaaat acagaaaaaa gtgctgctct
    10741 tgtccaaaag atgcaatata cgtctgactt agaaaagtca aatatacaat taaatcttgt
    10801 tcctataaat ttacctgaat ttttgaggca aaaggtgcaa gactatgaat tacaggctca
    10861 tcaaaaggaa gttgaactta tattgaaaat gcaggggaat atacaatccc caattcaaat
    10921 cgacgttgat aggttgacac gaatttttga taatatcata tccaacagtc tgcaatacac
    10981 tccatcaggt ggtaatatta gtattactgt aaaggatgaa aaaaattgta tttcttatga
    11041 gatttgtgat tctggtagag ggtttagctc taaagattta aaaaaggctc ttgataaatt
    11101 ttatcgagga gatgaagcta ggcagacaaa aggtggacat tctggtcttg ggctttacat
    11161 tgtcaaacaa ttagtggaac agcttggagg ttctgttaaa atagaaaatt caaaatcagg
    11221 tggagcatgt gtgaaatttt ggcatagcat ttaactatat attttaaatt aaaggagagt
    11281 gtctcaaagc gaaattttta attcgtgagg cactcttttt ttgtgaagtt aaatatattt
    11341 tcattatttc gacaatgaaa aaaggcttat ctctattttg agacaggcct tttttaaagc
    11401 ttatattata gttaaaaaga tttattagtt agatttgaaa gaaaatagag acagtcattg
    11461 ataatttaat ttatattatt atctgtaaaa tagttataaa ttgatatcta tacagtcaat
    11521 tatattcatc tttttaactt ttcttaacgg aataagagta gctataatac atgcaagtac
    11581 agaaataata gtagcttgta aaattgaagt aattggtata ttagtgaaat caagcttttc
    11641 tatagttgcc atgtttatga taatagcact ggcataccca gcaattgaac caaatatagc
    11701 agcaaaaatt ccataataaa caccttccca taaaaacatt ttatataagc tcctattact
    11761 cataccaata gctcttttaa ctccaatctc attagtccta gtatttatat ttgtatgagt
    11821 tgtgttaatg atgtttaaag taccaatcaa tcccacaaat aatataactg cccatgctag
    11881 aaattctatt tgtttaaaac tctctttaag ttgtttatct gattcaatat atgatatcca
    11941 atgagagcca ggattatttt cacaaatctg ttcaatagac aatcttattt gttctaattt
    12001 tgatttatct ttagcataaa tatttatctg gttgaacttg tttttatttg tcaaagtgtt
    12061 ataaacagaa tcatatacaa taacatcaac accatttatc cagccattgc cttgtaaaaa
    12121 gaacatttta tctactgtac tatatatatc tagttgtttt ttgttgattg tgatactatc
    12181 tttaggtttt aaatttgtat atctagtttt tacacctggt gtagaaatag caatcggatt
    12241 ttttataaaa cagatagaac catttttaaa gtcttctagt atagaatctg taatagaagg
    12301 cattaagtct tttaaagttt gctcatcaat tccaatgatt tttaacatct caccactatt
    12361 ttcaaaattt atatctgtat ctatttcacc ctgtttgtat tcagagtact ttataaagga
    12421 agtgcgattg acgtttttca ttttagctat tttatctagt gttgatttat caaaaccaga
    12481 tatttcattt gttaaggaaa aatctccttc ttttaaatta cttactgacc tagatacatc
    12541 taatacatta gagaactcac tcagtgcaac gaatactgtt atactcataa ataatgatag
    12601 tatagttatt atagttcttc ctttatttct ttttaagttt agccatgcta gatgtctttc
    12661 aaagtttttt attgtttttt ctcttttaat ttttcttttg attttggtag tactaccaac
    12721 catagcaatt ttgggagaga catgtgaagc atatattgct gatggtaatg ctgaaataaa
    12781 tgaaaaaatc aaagatacat tagtgcttaa tactaatggg aatatatatg ctgttgtatt
    12841 tttatgtata agctcagtaa tttctttatt atcatttgct agaagtatat ctggatttaa
    12901 gatattagtg accattccag ttatgccttt agaagatata attccaatta cagctccaat
    12961 aggtatggca acagtacata aaattaagat ttgtaaaata actatcttat atatctggtt
    13021 tggttcagct cctattgctc ttaaacatcc atactcttta attttctttg taacagagat
    13081 ttttaaaata ttataaatta caagtccaga agccagtaaa accaataagg caacaaataa
    13141 agataccaat ataatcatag atataccatc actattagaa ctattttcat ctttttcaaa
    13201 ctgtatctta agagcattta agtaaatcca attatattga attgaattgt gagaaagatt
    13261 tattttttta gctaattgat ttactatttc ttgaaacttt tcttgctgtt ttattttaaa
    13321 atccaaagag gataaaatat atctcttagg taatagattt tctgatgtac ctttacctac
    13381 aataccattt actataccac ttacatatcc tgtataatca tcctctaaaa tgccactcaa
    13441 tttaaagtta gctgtgtaat tatatggtgg tatggtatca tttaaaagtg aaatatctaa
    13501 attcataggt atagtcactc ctagtcttgg ttttataccc ataagcttta gtgtgttttc
    13561 atctaaggct atttcgtttt tatctttagg caaatgtcca gattttaatt tcattaattt
    13621 tgggtagtta gaaagtcctg ttttatcata ctcttttaca agtacagaga taccagaatc
    13681 ctttatttta ctactgccaa tagttattga agtaccagct tgataaactc tatcatcttt
    13741 ctttatgtca tcaacttgat ttttagtgag ttggtgaaag gatacatgtc tatcaccatt
    13801 aaaagaacgg gcttgttcaa ttcttaaatt ttgtaggata ccaattgact gtccaaccac
    13861 agttgtcata atagttgata gtactattgc tataatgatg aatgtagtca taaatttctg
    13921 gctttttaat tcttttattg aaagtgaaag gtaattcttc atttctattt cacctccata
    13981 agaacaccat caataattga aatttttcta tcagcaagtt ttgcaattct atcatcatga
    14041 gtaattacca ctaaagtctt tccaaactgt tttgaagttt ttataagaag attcataact
    14101 tcactgccac ttttactgtc caagtttcct gtaggctcat cagcaaatat tatatctggt
    14161 tttgcaataa gtgctctagc aattgcaact ctttgttgct gaccgcctga aagttcatgg
    14221 ggaagatgat tgagtctatc tgcaattcca agtaatgatg aaatttcatt taaatagtct
    14281 ttatcaggtt gttttttatc tagaagtaca ggcattgaaa tattttcaga tgctgtaaga
    14341 acaggtaaaa gattaaattg ttgaaagacg aatccaaagg attttcttct aagtgcagat
    14401 agttcttttt cagtgtgttt atatatatct ttatcatcaa gtaacacttg tccagaggta
    14461 ggattatcta atccacttaa aatatgcaaa agtgtgcttt ttccacttcc agaaggtcca
    14521 attacagaaa caaattcacc tgagtttatt tctaaattta cattattaag tgctataact
    14581 ttattgttat tagaaccata tatttttgat aattgttttg cttttataat cataaaaaac
    14641 actctccttt tctatataag aagagtgtaa gttttaaaac tcaatttttt cccaactttt
    14701 agcttaaatc taaaaagtct tcactatttg cggataattc gcagtgctga taagcagttt
    14761 cagtatcatt tttaatttaa aatgtaattt atgcactaaa ggctttacaa tacaactcta
    14821 ttgctttaaa actattttac acataaactt gtggataatt ttttatgttt tacttcctca
    14881 ttattatata aattattttt gtcacaaatt ctaagttgtc acataaatat actttttata
    14941 tcacgattta attcttttga ttttatcata attgctattt cttcaaaaga agaaagaaaa
    15001 gccacgatac atatcataat tcctgcaaca tcaagaccaa agcaaatata gagtaatgga
    15061 aaagcaaaga tagaaattcc agtgatttta ttcatcaaag tatgaagcga tgaaaatgtg
    15121 cgatacttaa aaaaaccaat actgtaacta atcaaccgta acaatgctat tgcaacaata
    15181 caccaccata gatacatggg aagagcaata ttttttccga cagtaaccaa aattgtaacc
    15241 gcaaaaatga aatcagcaat actatctaac tttgcaccta ttccactttg ttgtctcaac
    15301 tttcgggcaa taagaccatc aagtatatca ctaattccac accaaaaata acatatccag
    15361 aaaggcagtg aagagaatgt agaactaagc attagcaatg caaacacaat acgagagaat
    15421 gtaatattat tagcaaaact tttaatataa tcacctccaa aattctaatt tcatactatt
    15481 ctacatattt aaatatatat ttctattatt atttcaaatg ccttttgcca aatattataa
    15541 ttgaagcaac tcttacaact tctacaaaat agtatatata ttggttgtaa attgaaatac
    15601 attttataaa ttctaaacta acaaatttta tataagaaca gtgaaagctt acaaaatcat
    15661 tttaatttct tgttgagaat ttagcagtaa ttttagcact gttagtagaa ttattttcaa
    15721 tagaaataga gccaccatgt ttttcgcata atatttttga gatataaaga cccataccaa
    15781 agtgtgcatt gctgtcattt aaagtcttat ctctatagaa aggtcttaaa gctaatttta
    15841 gagattcctt actaaatcct ataccatcat cttcaattac aaaggtaagg agttcttgtt
    15901 caagatatat aagtatggat acattatttc tagcatatct aaaagcattc gatattatat
    15961 tttcgacgac tctaagaatg atattttcat caaaaaatag atttatatta tttatttgac
    16021 ttttaagtgt aaaagatttt ccttcttgtt tagcaatgat agatatattt tcatctaagt
    16081 tttctataaa cttgtttata ttcgacatag attttgaaac aactaaatct tctaatcgtt
    16141 gagcattact catattttct acatagtatt caattctgtt aatatgctca gacataagtt
    16201 gtgtggttga taaaatcttc tcatcactaa gcttacctgt tggtatgtat tttgttaaat
    16261 agtttgagta accttttaga actgttaggg gatttcttaa atcatgagaa aaagcagcat
    16321 taagttgttt tctatcatta atcatagtcc acatctttat gttgttttta tttagttcag
    16381 acctcatttt ttcaaaagca ttacatagat tacctaattc atcattatta ttatattcta
    16441 agcaaaagtc taaattgttg ttggatattt ccattgcacc tttattgagt atttctatgg
    16501 gtgtttttaa ttttaccttg taaaaaatta tatcagctaa taataaaagt cctataaaaa
    16561 agataagtgg aagtacaagc tgaagtatgt tgattatatt aagtaatgtt ttatcataat
    16621 cactaaattg gttcacattg ttttgaacta tggttataat acctgtattt ataggtgttt
    16681 ttaaatactt atcttgtata gaattatata gattattaag taaaatgtat gcgaaagtag
    16741 taagcactga tgctataatt agtgaagata aagtaagaac aaagaaagct ttttttaatg
    16801 acatattatt tattttatcc atttataacc aactccccag actgtttcta tataattttc
    16861 aagagtatgt tttgatagtt tgattcgtat ttttctaatg tgttccatta taacatcact
    16921 atttccattg ctattgtatc cccagactct atcatatatt ttttctttat caaatacttg
    16981 tccaggattc atagagagaa gctctactat atcaaattcc ttttttgaaa gtgaaatctg
    17041 attattatat atacaaatta accttttttc gtaatctatt gttaaatcat tataaaattt
    17101 aattttagtt tgatttgact tcctttgttc acgtcttaaa tgggcagata ctctagctcc
    17161 aagttcgtct aaatcaaaag gttttacaat ataatcatca gcaccagcac gaaaaccatt
    17221 tatcttgtca gtattttcaa ttctagctgt aagaaataga atagggcatg atacataatc
    17281 tctaattcta ttacaaactt caattccatc tatttcaggc atgtttatat caagtagaat
    17341 gatatctgga tttttagata ttttttttaa tgcgtctttt ccactttgag cagtgtaaac
    17401 ctcataacca ttcatttcaa agtaatcttt taagagtgtt attatacctt cctcatcatc
    17461 tacgacgagt attttattgt tcataagaat gctcccttct aaattaagtt tgtatagtat
    17521 gtatatttta gtataaaaat ctcaataaat tatcaaattc ttagttatat ttcaaaactt
    17581 aatcattaaa gttagtatat ttttatatat taaataaatc aaggatgaaa ttattagaaa
    17641 ttttaataga atatatattt gatatttaat aattattggt atgcaaaaat atagctgatg
    17701 aaatcttata atttaagttg aaaatggata aatagaaagg aaggatttta attaaataag
    17761 gtagattaat taaaaagtat ataaattata aaaatgaatt aatctacatt aattagttga
    17821 tatatcaatt attattgttt ttttcagttg cttaaaatat acttgatata tcaactaatg
    17881 ttgtttttta attaaaagaa attttaggaa agataatatc aactaggaga gataatatca
    17941 acattgaaga aggtagagca agatttctaa ataattgtga acattatctg gcatgtgtgc
    18001 attggtgttt taagcaagta attcaataga aagatgtgac taaaaataga aagaaatacc
    18061 ataatccaaa tgtaaagcta gaagacataa taagtaaaaa agtaaagata tttaataaaa
    18121 gaaataacta atagtgatta aattagtaaa tgctgataat tagcataatc attagtaatt
    18181 attagtatta tttaattaaa ttaatattag cattattttt atgaaattaa ttttaaatca
    18241 aattaatgag aaccccataa atttttttaa cacttaattt atgatttatg gggttatgtt
    18301 ctgttttatt tcaaagtatt aagttcatat aaatcagttc ttctgtgctt aagaagtggt
    18361 aactgttgtc ttatgtcagg aattaaatct ctatcaatgt cagcataaat aatacattct
    18421 ttttcatcag cttgagcaat tattcttccc caagggtctg aaattcttga atttccaaaa
    18481 gctatataag aagcattcat atttctagca ggtgcagcac caacatagaa tatctgatta
    18541 tctaaagcac gcattcttat agataactcc caatgggcgg gaccagtagt catattaaat
    18601 gcagcaggta agataacgat ttcagcacct ttaagagcca ttaaacgtga taactcaggg
    18661 aaacgaatat catagcaaat agctatacca acttttccat attcggtatc tattacagtg
    18721 acatcatttc cagctgtaag agtatctgat tctttaaaac taacttttcc tgtaacttca
    18781 atgtcaaata agtgcatttt acgatgtttt ccaattagag ctccattttt atcaaataca
    18841 tagcaagtat tatagatttt tccatcttct aactcaggaa tactaccaga aactagataa
    18901 attccatgtt tctcagctaa tttacttaat atcgttgttg tttcacctgg atattcttca
    18961 gcaaatttag gaaaatattt attttcatat gggcagttaa acatttctgg caaaactaca
    19021 attttagctc cattgatggc agcttctgtt accatatcta cagcatgatt gatattttca
    19081 attttatttt ctgtagtaat catctgacat actgcaattt tatagaaatt catagaaatt
    19141 actcctttca atatttttat gttattataa catattaaat aaaaattaga tattcttaga
    19201 ttgacccaaa tgggtatgga gagaagtgaa taagaatatt aaaaggtata taaaagttat
    19261 ttttattata tatggaaccc atatattgac tttttttata tatgttgata gtattattat
    19321 agataaaata gaataattaa tagagtttta tagggttccg caattattaa tgattggtct
    19381 ggtccgagat aaaacccaca acatagtttt tgttgtgaac acggaaggac aaaaacctgg
    19441 gagatatttt ataatatttc tcaggttatt ttattggaaa aataagagac atatttgaaa
    19501 agagggatga atttatgaaa tggttatttt tgtttgttgc aggtttgttt gaagtgtttt
    19561 gggcttttca actaaaaaat tcacatggat tttctaaatt aactccaagt atacttactg
    19621 ttgttggaat gattgtaagt ttttattttt tgtcattagc actaaaacat cttccacttg
    19681 gaacttcata tgcaatatgg actggaattg gtaccgtcgg tacagctatt gtaggcatgg
    19741 ctattttaaa tgagcctatt agtatatcaa gaattgcatg tataggtttt attgtggtgg
    19801 gaattgtagg gttaaagcta ttatctacta attagagtat attaatttaa ataagctatg
    19861 aaattaaagg cgagaaaatg tttaaaaata atcaacattt aaaaagtctt tacttcatag
    19921 cttatcttat ttataagttt attatatttg taaattatat gtatatacta tttatctaat
    19981 aaaagagcag ttaaatccat agggtcaact attacaccat ctttataaac acgcatattt
    20041 ccagatgcta tttcatctat taagattact tcatcattgt agaatccaaa ttcaaattta
    20101 atatcataaa gctcaagacc tttttcagct atagtatcac gaactatgtt agaaattttt
    20161 tgagtcatgc ttttcattga atcaaattga gcttgagtca taatgtttaa agcttctaaa
    20221 ccttcagatg ttataagagg gtcacaacgg tcatcatctt ttaaagttgc ttctacgtaa
    20281 caatctagtt tagcaccttc ttcaacacat gaaccataac gacgtaagaa actacctaca
    20341 gctctataac gacaaataac ttctaatcca ttaccaaaaa cttttgctgg tagaacttcc
    20401 atagttgctt cttctaaatt agcacttaca taatgagttt taattccagc attgtttaga
    20461 atttcaaaga acttaacaga tgtctctaaa ttagctctac caattccatc tatacttaga
    20521 cctacactat tttcgcctgg gtcaaatact ccatctttac cagttacatc gtctttaaat
    20581 ttaagtaagt aatttccatt atctaattca aaaacatctt tagtctttcc ttgatacact
    20641 tttttcataa acaacctcca aaaagttaaa ttattatatt gaataaggca ttagtgtcaa
    20701 aaaagttctg atattcgcat gaaaatttat attgatacct tatatttaat attatatcac
    20761 aaatagaagg gaaatttccg aacaatttta taaaaataac aaaaaaagta cattttataa
    20821 aacttataat aagagaacta atataaaatg ttagtgtcgt ttatattgac acacataagt
    20881 tttaatataa gaaacttttg acacactttg acacacattt aatgataatt ttataaaatg
    20941 catgaataca atgcttacat atattggcat gaaagttgct ttagatattt aacatataaa
    21001 caatagggat atattctcaa ataaaaagta ggggtggtta atagtgaagt cagttagatt
    21061 ctatggtata agagacacaa gagtggagga tgtggatgta ccaaaaatat tagaaaaaga
    21121 tgatgtaata attaaagtaa aagtagctgg aatatgtggt tctgatatat caaaatattc
    21181 aaagacaggt ccacatatgg ttggagaaat tttagggcat gaattttctg gagaagtggc
    21241 acaggttggt aaagaagtaa ggagttttaa aataggagat agagttgctg tttgtccagc
    21301 tatgccatgt tttgaatgtg atgaatgtaa aaaaggcttg tattcaagat gtaataatgt
    21361 agcaataata ggaaataaag aacttggagg ttgttttgca gagtatacaa aggtgaaaga
    21421 gagaaattta ataaaaatac cagatgagat aagttatgag actgccgctg cgctagaacc
    21481 agtatgtata gcaggacatg gtctttttag gtcagaagct aaagttggag atactgttgt
    21541 agtacttggt acaggtccaa taggattatt ttcaatacaa tgggcaaaaa tatttggttc
    21601 tacaaaaata atagctgtgg atgtatttga tgagaagtta gacttggcaa aagagttagg
    21661 ggcagatatt tgtataaatg caaaagaaaa gaatattgta gaagaaataa aaaggttaac
    21721 tgatggagat ggtgcagata ttgttattga atcggctggt actccactga catgtggtca
    21781 agtactgttg ctagctaaaa agggaggtac agtattgtat gcaggtgtac catatgggga
    21841 tgtagctttg actagagagc aatttgaaaa gatagtaaga agtgaattaa ctgtaaaggg
    21901 aacttggttt ggaaactcct ttccattccc aggaaaagaa tggagtgctg gattgtatca
    21961 tatgcaaaag ggtgatatga atgttgaaaa attagtaaca catagaataa atttagaaga
    22021 agtaccagct tattttgaaa aagtatacaa aagggatatt ttctttggta agataatgat
    22081 taatatagat aattagatta gagtactaaa taataatcca tataaaaaag gttaagaaag
    22141 gtatgattca tatggcagga ataattatag ccacacatgg aaatatggct aaagcaatgt
    22201 tagaaagtgc tgaattaata gtaggcaaac aggaaaatgt agaaacttta ggattaaacc
    22261 atggtgacag catagatgag tttagtagaa agttagaaga tggaattgag aaatataagg
    22321 atgaaggggt tttagtattg cttgattttt atggcggaac tccatttaat acatctgcta
    22381 ttgtaataaa taaatttgct aaggtatgtg agttagaatg tttgacaggt gtaaatttac
    22441 ctatgctttt agagttattt ttaaacagag atactatgaa gttaaaagac ttgaagaatt
    22501 tatgtgagga agttgggata tcaggaatca aagatgttaa aactgtttta aagttgtaaa
    22561 ataaattaaa aatctggagg aataattatg agtaatatag cattaacaag aattgatgat
    22621 agattaatac atgggcaagt tattactgct tggtgtaaga ttacatcagc aaaaaggata
    22681 gtaatcgtgg atgatttagt agtaaaggac ccatttatag ttcaagtttt acaaatggca
    22741 gctccatcaa ctgttaaggt tgaagtacat gatgtagaat caggtgctga agtcttaaaa
    22801 tcacataatg gagatgaaaa tttaatagta ttggtcaaat accctaaaac tgtcttaggt
    22861 ttagtaaata gtggggtaga tttaaaggaa ttgaatgttg gaggtatggg agcagggctt
    22921 ggtagaaaaa gtttttataa aaatatatct gtgtcagatg aagaaaaaga aatatttaga
    22981 gagctaatat caaaagatgt taaatgcttt atacaaatag taccagatgc aaagaaaatt
    23041 gatgtaggaa cattattgta aagaagacta tcttgtaaat ctttaaaaga aaatagggag
    23101 gaagaatatg cacatttcac taattcaagc agtattaata gcaatttttt attatctttc
    23161 ttggagtccg tggttgactt atgttggatt ttttacatgg aatagacctc tattggctgg
    23221 atttgtaacg ggaattatat taggagaccc tgttcaagga gcaattatag gagcaggtat
    23281 aaatatgata tatctaggtt ttatatcagc agggggagct cagatggggg acccagcttt
    23341 tgcaggatat gtaggtactg ctttagctat agcatctaag ctagatgtaa gtactgcaat
    23401 ggcaatagct gtaccattgg gaactgttgc aactgtactt tggattggaa agatgacagt
    23461 aaattctttt tttgcacatt gggctgatag agaggttcaa aaagggaatg ttgatagagt
    23521 agcatttata aatatagtac caccacagat actgttattt gcaatgagtt ttataccagc
    23581 attattagtt gtttattttg gtccaaatgc gatagatggt atgttggaag taatgaatga
    23641 taatgtgtta catgtattta atgtaattgg ggctatgttg ccagcacttg ggattgctat
    23701 gaatttaaaa ttgattggaa ataagtttac tatgcccttt ttcatattag gaatactaat
    23761 ggctgtttat tttaaagtag atataatagt aatatctgtt attggagtaa tacttgctct
    23821 tacaattact tcaattaagt acggaaaaga tagtgctaca tcttaaatac atgtgagggg
    23881 gaaaatatta tgtcagatat aaaaaatgaa gtatctaaga aaacgttaag caagaaagat
    23941 gtaataaaat catggttaag atggtttttc tttgcacaat ctaattacaa ttatgaaaga
    24001 ttgcaatcaa cagctttttc acattcaatg ttgccagtac ttaaaaaatt atatccagat
    24061 aaggaagagt taaaacaaga agtggagact catctagcat tttttaacac agaacctata
    24121 tgtggatgtg ttatacatgg aataacaata gctatggaag aagaaaaagc taatggagca
    24181 gatgaaataa gtggagatgg tatgaatgct atcaaaacag gcttgatggg tccactagcg
    24241 ggaattggag atactttaac tcaaggagtt ataactccta tagtgttagc tgtttgtatt
    24301 ggtttaactg agggaggagc atcaattgca ggacctatat tatttgtaat agctcaatat
    24361 ataataatga cttcaatttc ttttggaatg tggacaaatg gatataagta tgggaaaaaa
    24421 gctgttgaaa gtatacttca aggtggaata gtaaataagg ttatagaagg ggcttctata
    24481 ctaggtacct tagtcatggg ggggcttgta ggtaggttta tcaatttatc tacaccaata
    24541 agttatacta gtggtgattt taaatttagt cttcaaacgg atttacttga taagatattt
    24601 ccaggattaa ttcctttggt acttacttta ttagtactct ttgcattaaa aaaaggtctt
    24661 tctccaataa aaataatgtt tatactgata gtagtgggag ctattacagg tattttagga
    24721 ctattttaaa taaatatata ttggtgtaag ttaggattct atatctattg attatatttg
    24781 taggatgttg gaaaatttat attaaaaagt ttggcaatta gggtaatttt ataatagatt
    24841 aaattttaaa cttatgtaag ctatatgtag tatttaatat tttaaaaagt atctcagaaa
    24901 gatttttaat cagaatgagg tg
//
LOCUS       DAYAOX010000037        24831 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.037, whole genome shotgun sequence.
ACCESSION   DAYAOX010000037 DAYAOX010000000
VERSION     DAYAOX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.037"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(299..649)
                     /locus_tag="ACGI57_001206"
     CDS             complement(299..649)
                     /locus_tag="ACGI57_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HFG9716291.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(665..1081)
                     /locus_tag="ACGI57_001207"
     CDS             complement(665..1081)
                     /locus_tag="ACGI57_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HFG9716292.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(1100..1291)
                     /locus_tag="ACGI57_001208"
     CDS             complement(1100..1291)
                     /locus_tag="ACGI57_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HFG9716293.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(1442..2257)
                     /locus_tag="ACGI57_001209"
     CDS             complement(1442..2257)
                     /locus_tag="ACGI57_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HFG9716294.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVKGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(2304..3242)
                     /locus_tag="ACGI57_001210"
     CDS             complement(2304..3242)
                     /locus_tag="ACGI57_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9716295.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(3239..4111)
                     /locus_tag="ACGI57_001211"
     CDS             complement(3239..4111)
                     /locus_tag="ACGI57_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9716296.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(4136..6064)
                     /gene="acsV"
                     /locus_tag="ACGI57_001212"
     CDS             complement(4136..6064)
                     /gene="acsV"
                     /locus_tag="ACGI57_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid activation/regeneration protein AcsV"
                     /protein_id="HFG9716297.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(6176..6553)
                     /gene="gcvH"
                     /locus_tag="ACGI57_001213"
     CDS             complement(6176..6553)
                     /gene="gcvH"
                     /locus_tag="ACGI57_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFG9716298.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(6640..8766)
                     /gene="acsB"
                     /locus_tag="ACGI57_001214"
     CDS             complement(6640..8766)
                     /gene="acsB"
                     /locus_tag="ACGI57_001214"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HFG9716299.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(8823..9629)
                     /gene="acsE"
                     /locus_tag="ACGI57_001215"
     CDS             complement(8823..9629)
                     /gene="acsE"
                     /locus_tag="ACGI57_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase/acetyl-CoA
                     synthase methytransferase subunit"
                     /protein_id="HFG9716300.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(9679..11046)
                     /gene="acsC"
                     /locus_tag="ACGI57_001216"
     CDS             complement(9679..11046)
                     /gene="acsC"
                     /locus_tag="ACGI57_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HFG9716301.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(11077..12021)
                     /gene="acsD"
                     /locus_tag="ACGI57_001217"
     CDS             complement(11077..12021)
                     /gene="acsD"
                     /locus_tag="ACGI57_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HFG9716302.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(12041..12811)
                     /locus_tag="ACGI57_001218"
     CDS             complement(12041..12811)
                     /locus_tag="ACGI57_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase accessory protein
                     CooC"
                     /protein_id="HFG9716303.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(12867..14252)
                     /gene="lpdA"
                     /locus_tag="ACGI57_001219"
     CDS             complement(12867..14252)
                     /gene="lpdA"
                     /locus_tag="ACGI57_001219"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFG9716304.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTIKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(14296..15177)
                     /locus_tag="ACGI57_001220"
     CDS             complement(14296..15177)
                     /locus_tag="ACGI57_001220"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFG9716305.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(15212..15886)
                     /locus_tag="ACGI57_001221"
     CDS             complement(15212..15886)
                     /locus_tag="ACGI57_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HFG9716306.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(15923..16795)
                     /locus_tag="ACGI57_001222"
     CDS             complement(15923..16795)
                     /locus_tag="ACGI57_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HFG9716307.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(16887..17519)
                     /locus_tag="ACGI57_001223"
     CDS             complement(16887..17519)
                     /locus_tag="ACGI57_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HFG9716308.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(17624..19300)
                     /locus_tag="ACGI57_001224"
     CDS             complement(17624..19300)
                     /locus_tag="ACGI57_001224"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFG9716309.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(19549..20328)
                     /locus_tag="ACGI57_001225"
     CDS             complement(19549..20328)
                     /locus_tag="ACGI57_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9716310.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(20638..22557)
                     /gene="cooS"
                     /locus_tag="ACGI57_001226"
     CDS             complement(20638..22557)
                     /gene="cooS"
                     /locus_tag="ACGI57_001226"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HFG9716311.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRLIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(23164..24075)
                     /locus_tag="ACGI57_001227"
     CDS             complement(23164..24075)
                     /locus_tag="ACGI57_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HFG9716312.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(24191..24769)
                     /locus_tag="ACGI57_001228"
     CDS             complement(24191..24769)
                     /locus_tag="ACGI57_001228"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFG9716313.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
BASE COUNT         7213 a         5142 c         2859 g         9617 t
ORIGIN      
        1 aaattgcttg atgaggcttt tgcagtattt aaagaatata taattattta acattaaata
       61 atccaatctt gtaataaaat ttactcatta aagtatatta cagaaaatac ctcaaaataa
      121 actttttagt tcatgatata ctcttttttg catgaaatta gatatatttt tatcatttca
      181 acaatgaaag ggaggcttat atccatcttt agacaacccc ttagataaaa tttttaataa
      241 ttaaacatca caaaatataa aaatcgctta tatcaaacgt ataagcgatt aattatattt
      301 acatatgttt tttagcctct aacaacattt tattagcttc ttccatatac tctatagcct
      361 tttcaatact ctttgctgtt tcttcttttc caatagctct agccttttca acccactctc
      421 taaatccttc ttcatgagtt tcattgtgat ttatccaatg tactaaaagt atttttaaag
      481 ttttttcatc cttagactct gatgattcct catgactatg ttcgtggtcg tggtcgtgag
      541 catggtcatg tggatgttca tgacatatat ctccatgagt atgttcgtgg cagtggtcat
      601 ggtcatgatg tccatgatga tatcttacaa tctctttatc aaaaatcatt agttattccc
      661 cctgttattt tatattaaac tcttttttta taaggtttac agaatctttt acaagtagtg
      721 ctaatggttt accttctaat ccaactaact ccacattttc tggcataata ggtaaaagta
      781 tcttaagtgc ctcactgtct gcaatagctt cacttatatt acaagtaact tctcccatca
      841 tagaattagg tattattaca gaaataggtg caactattat atttgctttt ttagctgata
      901 ctactatagc attttcccct gtagctcctt tattagcatg agctttcatc atagaactag
      961 ttgctataga attagtccct agtgcataaa tttctacata tgtaggtaac tcttctctca
     1021 aagaagaaac tatctgggct ccaatacctc cacccattcc atctatcact gctattatca
     1081 tatgaaattc ccctattaat taattttctt gtattattat cttatggtcc atcaatctta
     1141 tttctttaat tatcccatct attatctttt gctctcctaa taaatccgtt aaataaatct
     1201 ttccatcaca agggtctata ttaaccacat tttccataac cttttctaac tcattattac
     1261 ttttacaaat gaaagctgat gattcacaca tataaatctc ctcctaatat gatttttatt
     1321 aaaaatcaaa aaaagattcc cttaggtatt tcaatacacc aaagaaacct tcatgatttc
     1381 agtttttgta tattattata ccttaagtat atcactttaa tttatgtaaa gtcaattaaa
     1441 gttaacgctt tttattatac tcttcattct tgtatttttc atatagttct ttaactaatt
     1501 gcttctcata atcagtcaaa tcgcccttca caaactcaac attaaattgt tcagctaatc
     1561 cctttactat gtttttctgt agaatatcta tgtctatttt tctgccaatt tcattttcta
     1621 ttccacttgc ttttttagct gtaaatttca tagctctttc tttcaattct ggtgtcttag
     1681 tttttattaa tttaaacaat ttttctacat caaaatccag tattattgaa ccatgttgta
     1741 ataaaacacc atcttttcta ctttgagcac ttccaattac cttcttatta ttaatagtaa
     1801 cttcatatga tgcatgtgca ttaaaacatg cagctgataa attttcccta cttattctct
     1861 cgcctctatt taaattatct gtttctatac cacttagatt caacccttta actagacctt
     1921 cacttatata tctataagat aggttgatgc tcttatcgat taaaggatta tcttctccta
     1981 taattatact ataggttagc tcattatcat gtaatacagc tcttccacct gtaattcttc
     2041 tagtatagtc tatattcata catctacact tatctaaatc tatctcatct tccaatttct
     2101 gaaaatatcc tatactcaaa caagctggtt cccatgtata aaatctaaga gttggcttat
     2161 tatgtccttt cttatatgca gtaaatatag cttcgtcaat tgccatattc attgctcctt
     2221 catatgattt attatgtata actctccact gattcatgat tattacgcct ccatttttag
     2281 tataaatata tcctagaaat cctctataga tttatttctc ttattgctaa atctatctca
     2341 ctttgattta aaggtcttga atagttgtat atagtacttc ctggtctttc attatagtat
     2401 ggtctattac aatccttaca acctcttatt tcaaaaggat agcctttgtt tatgtctttt
     2461 aaaatatcat tgtcaagctt tatactattt aagtatccat ttttaaactt aaaatactct
     2521 tttggataac ctttatttat catataagac attaactgta cccttctata actttctatg
     2581 cttggttgat ttatcttttc cattttagtt cctctaactg gagtaaatgc aaataaacta
     2641 atcgttacat cattttcctt taaataagta tataaattgt atatatcttc atgactttca
     2701 cccataccaa ctattatatg tgtacttatt ttattgggat atgattttga catttcagtt
     2761 ataaatttta acttttcatc atagttattt ccttttattt tttcatatag ttctttattt
     2821 gctgcatcta ttgctattcc tattttatca actcctgctg aaaaaaactt ctttatctgt
     2881 tcatcatttt ctaattttgc agatagtgaa attggcatgt ctatcttacc acttatatag
     2941 tttataaagt ctaaaacaga gttatgtgct tcttcacttg ccattgattg aatacatatt
     3001 ctctttatat tttttccttt ataagctttt aaagcatcta atatttcttc ttttgaaaac
     3061 tctggccaaa ttactcttga taatttatct tttctagtac tactttctat tgattgagaa
     3121 caaaaactgc atttatttat gcatttttct ccaatcatta tatatgctgt ggtaggaggt
     3181 atatcgcttt ttttgtttaa tatcccaagt tctattgctg tcccaactga tagtctaatc
     3241 ataaaacgca acactcctta cactcattta ttgtcatatt catttctata gccttattct
     3301 ttgcagacct tgatggtaat actattctat tgactccaca cattatagat aattggtcta
     3361 gctcttttct atacactcct cttggtctca tacaacctag attaatttca gtatctggta
     3421 aattaattct agcttcacat aaaatatctg ctaccccttc taactcagga ggttctacat
     3481 tttcatactc agtccctttt gttggaataa ggactataaa tgtaagctta ttaggaggat
     3541 ttttttgtag gtattctata atttcatatt caccctttat ttgtcctcct tttaaaccaa
     3601 tacatatatg tggtgctact tctgtatgtt cttgtattgt attatagact tcaatataat
     3661 cttctttact ctttttcatc ttataaactt ctcttattgt ctcatcatca aacactaaat
     3721 caaaagaaac tatatctaaa tacttacaca actctacaat gctatcttta tccataagac
     3781 ctaaatgagc attcaatcta tatccttgct cttttaagtt tttaatggta ttaatatgag
     3841 tatttatagg gacatctccc tcataactac aaccgccact caatagaaag ctagttatat
     3901 tccttgattg tactttttcc tcatattctt ctattggcac catattgttt aagtagtgac
     3961 cgttgcaatg ggcacatttc aaactacatt gattgcttgt agtactaaga gctagtgtct
     4021 ggttaggata agcaaactct attttatttc caaaattctt taatttaata tcaaatgcta
     4081 ttttcatttt ttcagataga ttcttattca tagtttattc cttcttaagt acattttact
     4141 cttctagtaa tttctttgct gttgggaact gctctatatt cgtatgtggt aagaaacatg
     4201 cagaaataaa ttcatccata taacttggat atacactaag ttcaacataa gttatttgat
     4261 tacctatttc ttcaagttta ttttttgcat ctttacttat aagcgctaag taagaaccaa
     4321 caagtgaact attccctata taagtaaatt tttctctatc aatatcagga agtaacccta
     4381 taattattga gttttctatg tctaggttgt ttcctatacc acctgctata taaaccctat
     4441 ctaatatact aaaatccata cctaaactat ctacaagagt gtatgcgcct gaatatatag
     4501 ctcctttagc tctgatgaaa ttatctatat caacttcatt gactactata tcattttcta
     4561 aatcaaattc ttctttaaat gctaatacat actctcctat ctcatgctca ttaaatctaa
     4621 ctctcttatt atctaaatcc ctgtatattt tacctcttct atctattaca cctttagtaa
     4681 tcatttgaca gattaaatca attataccag aaccacatat accaactggt gcacattcat
     4741 caataatctt taatgttggc tctaaagtat ctttatcaat aattactttc tctattgctc
     4801 ctgctgatgc tctcattcca cagcttatcc ctccaccttc aaaagcaggt cctgctgaac
     4861 atgcacagct catcatataa tctttatttc caaatacaat ctctccatta gttcctaaat
     4921 ctataaataa aacattttct tcacttgacc atatccctgc tgataataca cctgctgtta
     4981 tatctcctcc tacataactt gcaacagatg gagctaagta cacatatgca ctgtcattta
     5041 caaaaagacc aacattttca cccataagtt ttggagattt taaaaatggt ggtatgtaag
     5101 gttcttgtct taagaagtct gtatacacac ccaagaataa acttgtcata gttgtatttc
     5161 cggcagccac taatgtaact acatcttcct tatctattcc atttctttca tatattgttt
     5221 ttaatagtgg atttatagtt tcctcaacta tagctttatg aagtgtctct aatccatttt
     5281 tcttagttga atatataatt ctatttataa catctgctcc atattttatc tgtgcatttc
     5341 cagaagatgc tttatctact acctcttttg agtataggtc tactaaacaa acaactactg
     5401 aagttgtacc aatatctatt gcaactccgt ataaggaatt ttctttattt ccttgttcta
     5461 tatttatgat tgtaagtttc ttttgttttt gaacatatgt tattgtaacc ttaaaatcac
     5521 tctttctaaa tacagttggc atttttctaa gtatatccaa tctaaaatct atttcattat
     5581 atcctaggtt atttctaaca tatctttcca atctatctac atcacttata ttatcatcta
     5641 aatttggttc ttccatttct atgtatttct tttttatatt tgttttaaat tgaaggttat
     5701 gttcttcaat tattttttta gctctttcaa aaatttccct atcctctttt ttattactac
     5761 cttctatctt cataccatgc atagaagatg atactttaga aggaacttca atttctatat
     5821 ctgatattac ctttgtacaa caagctagta tataaccttg ctcccactca tcatctgtta
     5881 tatgtcttgt tttttctgta tcaacttttc cattcaataa ttttacttta cacttcccac
     5941 aagacacgtt tccattacaa ggagcatcta tgaatatatc tgcatttctt gcaacctcta
     6001 gtaatatatc cccctcatta caataaacct ctttattgtt tggtgtaaaa cttactttta
     6061 tcatgttatc cctcccaatt tgttctatat atgataaaag gaaagtatag ctaaatttgc
     6121 gtgcactcaa attttactat actttcccca attataataa tatcttaatc tttaattatt
     6181 ctaatccagc ctcgtattct ttatcactta ataacgtatc aagttcagcc acatcagcta
     6241 gttttacttt taatatccag ttttcatatg ggtcttcatt tatacattct ggctcatctt
     6301 ctaatttttc attaacttcc aatacctcac cattaacagg agatattaaa tcagaagcta
     6361 ctttagaaga ctcaactact ccaaaatcta ctcccttagt tacagtatct tctacttctg
     6421 gtgtttcaac aaacaatatt tctcctaatt gatcttgagc atagtctgtt atacctatat
     6481 atacaacatc tccatcaatt acttttaccc actcatgatc tttagagtat tttaattctg
     6541 gtaataattt catttcgata gacccccaat attatattat tgattaaatt acttaaatca
     6601 tattaatatt atatcatatt attttaaatt ttccatcaat tacattacac tttccatagc
     6661 taatgctgga tgaccttttt cttctaagaa agctaatact ccttcagaat cgtcagctat
     6721 agtttcatca caaaccatat cacagaagtt ttctattcca gtcatttctt taactgtagc
     6781 atttaattta tcagctacat aatcttttaa ttcttttggc atccaaacta ttctttctgg
     6841 tccaccttct gcataagcga attttttaga agatataaaa tgtcttccgt gtcccataaa
     6901 tcctggagtt tgaactcctc cacctgtcat agaagcaagt tctccgaaag tcataccaac
     6961 tggagtaaca cttgcaaatt ctctgttaac tacaacaaat ccatttgctt ctggcattat
     7021 accacagata cattcgaagc atccacaaga agtcattggg tcttctagta tagagtataa
     7081 tgtaacagat tcaactgcac cttgagatat ttggtttaca gtttcattta ctgaattcca
     7141 tacacctgtt ctatcatcta agcactcgcc tttttctatt ggttgacaag gacctgttgg
     7201 gtctaattct ttagtagcct tagcatctaa ccagctaact gctccacaaa gaccaagtct
     7261 ttcaggagta acaacacaaa cgtgagctgg tgcaaatgat tgacataagt tacaagaata
     7321 gaaagtatca acactttcat caactaatga agctaatctt tcatctctag cattgtactt
     7381 agctattgct tctcctttta attcagaaac tttttcagca tctgtaatta tagttatttc
     7441 acacttatca actactgatt caaattcatc taacatctta gcatataaaa cttctcctat
     7501 atgctcaagt ctaaatcctt tatcaaatgc atctttagag attcttaccc aagccatatc
     7561 tctttgtcca acgtgcatta caccttctat atagtttaag aagtagtgga aacgtctttc
     7621 aagaactggc tcgaaatctt cttgcatatt tttaccagct attttaacta ttacagcaag
     7681 tggtaatctt aatacaccat ctgcatcaac ttcatctata tttggcccta ttatttctat
     7741 cttatggtct tcaacttctg ttgcttcttt cttaacaaca agctcccaag cttcagttct
     7801 gtttccacca aactcagcaa acatatcgct ctttctaatt ctctcacctt caaatgctgc
     7861 tgcaaatgaa actgggatag gtatttcagt aacttttatt tttattcctc tagcttctaa
     7921 tgaagtagct actatcttat cgtaatcttt ttgagttaat aggttcattg gaacttcaag
     7981 aactgtttgg tcagttataa ctgggaatcc taaagctatt gctccagcac ctgcagatac
     8041 aactaattca cttaatggtc caaatgcatt aacaaatgca ggaactctat tagctgtgta
     8101 ctctaataat ccgtttaaat caccaggagt taatccaccg aatattaatg ctgctcttac
     8161 tgcaacagat acaacgtgta taacagaagt tacatcatat cctagaggta taactcttag
     8221 ctcaagaccc atttttactc cagcttctat agcttggtct ataactttac caactaagaa
     8281 agttagtaaa cctttagatt ggtaatcttt tataactttt gctgctgatt ctgcatctgg
     8341 acactcacca agaacaactg caacaccagg tatatctcct gtaactaatg gtacaccaag
     8401 tgatctgatt atagggtcag ttatatgacc tgcacatggc tcactatatg gagcatccat
     8461 agttgaatat tttaatgctt caattacttc tgctgctaaa gctgtagcta aaccagcatt
     8521 aaaagcatgt tctagtaaat gagttctatt tattaaagat tttactactt ctaaagcacc
     8581 ttctaagtcc cctaaagtgt tcatcttttg tcctgttgca gcatatatac aaggtaatga
     8641 atatgctgta tcagggaaag caactttatg ctcttttcca ttcttttcta tagcttcagc
     8701 taacatagct tgagccgcac ctaaagcttg ttctgaccct gtaaagatta tattatatag
     8761 attcattcta tttccccctt taatatttac acctattttt tattaactat accctaaaaa
     8821 ccctagaaag caaatccacc ttcatttaac tctaagaaag agtctgcata taaagatgcc
     8881 ccgttgatta tgtcacaaga ttttacagtt ttcattaatt ctgggtcaca tggattcatt
     8941 atagctgaac taaatccatt agccattgcc attgctaaga atgcactatc taaaacaggt
     9001 ctaacatgct taggacatcc attagatacg ttagataatc ctcctgtagt taaaagtccc
     9061 atctcagtca tcatttttat agcttctaaa acttctactt gtttttcttg catacctttt
     9121 ataactaagc ataatgggtc aaatagtata tcagttggct ccattcctaa cattaagcct
     9181 ttttctaata tttcttggca gtaagccata cgctcatcat tatctcttgg gataccttct
     9241 tttgcacata aacctataac cattgcttcg tattcagctg ctatatctat taaatcaaat
     9301 cttgagccag catcagcaga gtttattatt ggttttgcat tagttctatc ataaacttta
     9361 agtccagctt cgatagcttt tttattagct gtatctaatg ctataggaac attgttgaac
     9421 tcagattgaa gtaatttgat tccccatgtc attatttctt cgccatctct ttcggcaggt
     9481 cctatattaa aatctatata gtgtgctcct gcttctaatt gctcttttgc tctttttaag
     9541 attggagctg ggtctctttc tgctaatgct tttcttattg aaggtgatat acaatgtatt
     9601 ctctcaccta taatcataaa tttttccatt tataatgtcc ccctttaaat ttaatattaa
     9661 tagttagtta atattttatt agtttgtttg gcatgctttt tcttggtatc cttttaagaa
     9721 cttaggtaat tccatagatt cctctggtcc tataactaca ttccatcctg gtaagttatc
     9781 ttctatatca cctttaagta ctgcaacttt tcctggaatg ataaggtctt tacaattagt
     9841 tacttcttct accttacttt ctttaatgaa agcagagatt gagtttccac caaatttacc
     9901 agcagcccaa gatgtaagaa ctgaatatcc acctgcatct ggtattacta accatactgg
     9961 aacttttgat ctttctatat caccagcaac tatgaagtaa gttaatgcaa agtcaacagt
    10021 aactaatact ggagaatttt catctggatt gttgattgga taaatctttg gttcaactct
    10081 cattggtctt tgtgggtcag tgtatatgtt ttgtcttagt gcaaataatg gtaatgcctt
    10141 agcataacta atatcatcta ttactattat agaaccgtat tttatagtga atattgatga
    10201 taaagcaact tccatcatag ggtttgaatt agataatcta tttgcaaata ctattgaagg
    10261 atatccaaat gttctatctt gctcttttaa agctgttctt ctaacttgta tagcattagt
    10321 gtaagtatct tttatgtttt ctcctgttac atctaatact aattctttat atccagcggc
    10381 ttgaattaat tcaactgttt catataattc ttctaagcta ccagctttta cacctaatgg
    10441 taaacttgct cctttaacta cttctatcat atctttgtag ttctctttag ttgctccata
    10501 taccattggt ctagcatctt ttaatacttc aacagcttct ttaactactt gtgcatcatc
    10561 acaagctaaa atataagcta cttctaatcc actgcctttt attttatcta ttaattttaa
    10621 atacgcatct ttatctccac aatactctaa aacagccatt tcaactttca tttgttcacc
    10681 aattcttaca tagtcaactt ttttcatgtt agctatttta gcatctactg cttcatcact
    10741 catacaagta cagaatgaaa ctgcatatct atttctactt actaatgttt tttcatgtct
    10801 aaataatact gtttctccac ctagttcata ctcagatgca ccagcaccaa cttttaaagc
    10861 tttcattaaa ggtgcagtag cctctgataa ttttgctata gcgtcatcag acatatgtgg
    10921 gcatttacct acttctacag ctcctgaagc aaccttcata gagaaagcca tacaagttgg
    10981 gaatccacaa tccttacagt ttttctttgg tgttaattta aatatatcta aagcttttaa
    11041 tgccattatt ttttccccct aaccttaaca atttaattaa gctaatgcgt taaccaattc
    11101 ttttatagtt tctattgatt ttggatgacg aagtataact gcgtttgaac cacctactaa
    11161 aacacttgct gcagttgaaa cttccattgc tatagttctt tcttctggac aaccccaatc
    11221 tggctcatct tctattggag ctatagcttc tttaacatga cctacttcaa aagctactgg
    11281 tgttatgata ggcatttgta atgtcttatc attttgacca aatgctgcaa gtctaattct
    11341 atccatagta gatgcaacgt actcatatcc ataaccaact gctgaacatc caacattcat
    11401 aactatattt tcacctttaa cgcctagttg agttaacaat acgtttaatt gtttagctag
    11461 gtttatatca acagaagatt cagctcctac tttatgtgca taagccatac cagctgatgc
    11521 tcctactcct ttgtagttat cttctactgc tgacataaat aagcagttat gtccatccaa
    11581 tgtttgagct aatttttcaa ataatttacc atctttttca tgatttcctg aaccagctac
    11641 aactaaaggt aattttattg cttcaattac tgccttagct acatcagcac attcatcaac
    11701 tgacttatct aatccatttg ggtcagaacc atcaaatttt aaacaaataa aatctgcttg
    11761 tgtattagct tcaacatatt tagcccactc tactggacaa ttagctacat ctttgtataa
    11821 ttccttatat gaatcagtcc agctttcagg ataaacgtct gatatttgta tacctatttt
    11881 tggagaatta cctacttctc catcaaaact ataaaaaggt aatacatttt ccccacctaa
    11941 tttaattgct ttttccccta ttcctacttc aacttctgat atttttccag aatatttttg
    12001 agtagacatt ttaaatgcca tatttttttc ccccttaagc ctattttaat tctaatttag
    12061 caaaaatttc tttcatagcc actttagatt ttgaatcttc aggtaaatta actaaaggaa
    12121 tacctgtaga atcatattca tatatcaatt catccatagg tacaacaccg attagctcta
    12181 ggttatgctt ttcaatttct tctttaacac catcattcat aaccccattt ggaactttat
    12241 ttacaatcaa aagtattctt ccaacactta actttagttc ctcagctaaa tctcttattc
    12301 tagcaacagc ttgtatgcta cgtcttgagc aatcactaac caataaaagt gtatcaacat
    12361 gtctagtgac ttttctactt agatgttcca taccagcttc gttgtccatg actaagtatt
    12421 tgtaatgacc agatattttg tttacttgtt ctctaagtat cccatttaca aaacagtaac
    12481 atccttcacc ttctgaccta cccattacta ataagtcata tccttcgcct tcttcgatta
    12541 tagaatttaa tctaaattga agatattgtg cctttgtcat accaccaggg aatgcattgc
    12601 ctaacttttc tctttgattt acttcttctc ttatttctcc tattgttgct tcaacttcga
    12661 tacctaacac ttcatttatg ttagcattgg catcagcatc tacaactaat actggtccct
    12721 ttttgtcctt aaccaaataa tcaatcaaaa gccctgtgag acttgtttta ccagttccac
    12781 cttttcctgc aacagctata ttgtatccca ttttaagact tcccccttaa atctagccac
    12841 agagccttat gtactctgtc tgtctattat aatttaggta cagaatgaac acattctcca
    12901 tgtacatcat gaagtgcttc cattagccct tcagataaac ttggatgtgg atgtattgca
    12961 tcccctactt gttctactgt taatcctaga tgaacagcaa gtgacaactc tgtcaataaa
    13021 tctgtagcat gaggtcctac tactgctgca cctattatct tatcagtttc tttgtcagct
    13081 ataactttta caaatccttg gaaatgtcct atagcttgtg cttttccaag acctctaaaa
    13141 tcaaattgac ctacattgta ttctacacct tcagcttcaa gttgcttttc agtcttacca
    13201 acaccagcaa cttcaggttc agtataaaca catcttggaa ttgctctata atctactacc
    13261 ttagtcttac ctaaagcatt ttcaactgct acgatacctt ctttagaagc aacatgtgct
    13321 aggaaaggag tatctattat atcgcctatt gcgtatatac cttctacatt agtttcaaga
    13381 tgttcattaa ctactacttt tcctctttcc atttcaattc ctatatcttc tactccagaa
    13441 ttgtcaaggt taggtcttct accaacacat actaatgcat attgtgcttc tataaccttt
    13501 ccattagaaa gagttgcaac agccttacca tcaactactt cacaagtttg tactccaata
    13561 cctgtaataa ccttaatttt atctttctta aattgtctaa gtagttgttt agctacatct
    13621 ttgtcttcat ttaatagtat ttggtcaacc atctcaacta tagttacttc tgttcctaag
    13681 gctctaaaga actgccctat ctcacatcct ataactccac cacctactat taacatagac
    13741 tcaggtatct cttcaagtcc aagaacttca tcactagtta ttacaacttt tccatcatat
    13801 gggaacattc ttggtacaac tggaacagaa ccatttgcta gtattatctt atctgctttt
    13861 atagtttcta ctgttccatc atctttagtt acttctatag tgtttttatc aattaatttt
    13921 ccaaaaccat taactaagtt tactcctctt ttttcaaata agaactctat accacttatt
    13981 aattggttta caactttatt ttttctttcc attatggcag tgaagtttgg cttaattgta
    14041 ccatctattt ctataccaaa atcctttgct tcttttacag tattcaatac tcctgaagaa
    14101 gcaagaagtg ctttagttgg tatacatcct gcatttaagc aagttcctcc aactcttctt
    14161 ttttcaacaa ctgttacatc tgcaccaagc atagaagctt ttatagctgc tacatatcct
    14221 cctggtccac cacctactac tactatcttc atctttaatt cccccaaacc taatataatc
    14281 tacaatcttg caatactata aacctgcggc atccagtata gcaggtatat tctcttctgc
    14341 cccaattgcc attatatgaa caccatcaca taaatcttct tcttttaatt gtttgataag
    14401 ttcccctgcc atttttattc cttcacttac ccagttttct tttccagctg ctcttaatct
    14461 ttcgatttgt tcatctggaa caaatattcc tggtacattt gcagtcataa atttagccat
    14521 tcctggtgat tttaaaggta ctattcctgc taataccttg caatccatat cccttgttaa
    14581 ttctctaaac tttctcattg tatttatgtc ataaagtgct tgtgtttgga aaaatttagc
    14641 ccctgctttt attttctttt tcatttttaa taattgaact tctattggag aatattctgg
    14701 agttactgat gctcctagat aaaaatctgg agaacctttt aacttattac cagccatgtc
    14761 cgtacctgcc attaaagtct cagcagtttg aagtatcccc acagaatcta aatcaaacac
    14821 tcccttagct tgtggatggt ctcctacgct agtatggtct cctgttagtg ccaacagatt
    14881 gtttatccca aatacacctg ctgacaacat ttctccttgt atagctattc tatttctatc
    14941 tctccctgtt atttgaataa ctggctctaa tccagcatct tttaataatt tacatgtagc
    15001 aagagaagtt gctctcataa ctgccgattg aaagtctgtt acatttgctg catgaactct
    15061 tccaactaac ggctttgcac attctattaa atgtgaaagg tcagtccctt ttggaggagc
    15121 catttcagta gtaactgcaa attttccact ttctaatgtt tctcttaata agctcatctc
    15181 taatccccct tttaaccttc taaaaaaact tttatgaatt tgctgctgct tctttctttt
    15241 tctttgcact taaacttctt ggattatttt gcttagaata gtcttttggt ggtcttattt
    15301 ctgctaagtt atctaattgt cctatagctt ctagtctttc atatatcttt atccaagcac
    15361 aatcattctc tgagtttact tcacacttac catttttagc tccaccacaa gccccattca
    15421 taagaccttt agcacacata gttactggac atatcccacc tgtccaacca agttcacagt
    15481 ctccacaagc cttacaggct tcttcatatt ctccaactct ttgtacttcc cctataaaca
    15541 aagtattgtt ggctggataa actggcttat cttttaaatt cttaactatt gtctgtgtac
    15601 catctccaca tgctaaagat aaaactgcat cagcttcctt agtctcttct tttagagcct
    15661 ttaaatcctt tttagacttt aaaaggttac aagcagggtc aagcattaca tatcctaaga
    15721 tttttttgcc ttcaccttca agagtttcct tcatttttaa aacttcttct tccccaccac
    15781 ttttacaagt tgctgcacat tgattacaac caactaaaac cagcttgtca aaatctttta
    15841 agtaccctag cacttcttct aatggtttat tttcagaaat tatcatatct acttcccccc
    15901 taaaccttcc acatatatat atctatttat tgttttgtaa tttgcaagcc ttaactacat
    15961 gttttgcaag tatagatgtt gtaactgaac caacccccgc tggaactgga gttatcattg
    16021 atactttgtc taaaactgca tttgtatcaa catctccaca taaatttcct tcttcatcaa
    16081 catttatacc aacgtctata actaccgctc catctttaac aaaactttca tcaaccatct
    16141 tagctctacc aatagctgct attagcacgt ctgcctctgc tgctacacct gatagatttt
    16201 tagtttttga atgacatatt gtaacagttg cgtgttcact taataaaagc attgatactg
    16261 gctttccaac taccattgat cttccaagaa ctgttacctt acttcctttt aaatcaacat
    16321 tatagtgttt taatatctca actactgctg taggtgtaca tggtggaaat ccacttttat
    16381 ccccttccat taccttagca gagtttattg gactaaaaca atctacgtct ttttccggtg
    16441 caataacata ttttataact tcttcattta agtgttttgg aagaggtcta aaacataata
    16501 taccattaac atttttgtca tcattaactc tctttaatac atcaatatat tcctcttgtg
    16561 ttatatcctc agctaactct aacacttctg ttgttatacc tatgttttga catctcttta
    16621 gtgctcctct ttcataagat aaatcatcac ttcttgcccc aactctaact attgttagtt
    16681 taggatttat accttcttta attagtaaat cgacttcttt tattaattct tcactaatct
    16741 tatcagctac aggttttcct tttataattt gtcctttagt tgacattcct tccatacatt
    16801 cacaactccc ctacaagcaa acttaaatta atttattcta tataccacat attaataaca
    16861 tagtatctac tttcgtcatt taaaaattat tttccaagag ctttttcaac tttggcataa
    16921 acttcatcac aaatttttac accatcttca actaacttat ctacttctgt ttttacctta
    16981 ttaacatatt ctttatcttg tatagaattt atatttataa ctacatttaa ttgtccacca
    17041 agtatagcag ctcttaaaca ctgaactcct acaccaacat cacttatagc aagtcttgaa
    17101 cctttatcaa ctaaatcttc atgtaactta atagcttcat agcaaactct tactatgttt
    17161 aatggtactt cacaagcaac ttttaatgcc ttttccattg tttctgcttt tattcttttt
    17221 tcttcttctg tttctttagg aagcccgtaa gcttttgata atggtaagaa acattctgca
    17281 tcatcatcta tcatttttaa taaatctttt tctaactttc ctgccttact taatatctct
    17341 tttacacttt cttcatactc agcatacttc ttcttcccta ttgtaaggtt acataccata
    17401 ctacctaaag ccatacctat tgcacctact agtgcagcag ctcccccacc acctggtact
    17461 gcttctttgg aagatagtac ttctacaaaa tcaacacaag ttttatctgc tattttcatg
    17521 ataatcctcc ttgaaaggat gtaggaaaat atctcaaatt gttcgagata ttttacgaac
    17581 tattttaaga tatctcctac tctttattta taaattaatt tccttagaat aatccagcta
    17641 ttactccatt ttcatcaaca tctatttttt ctgctgctgg aacttttggt aatcctggca
    17701 tcttcattat ttctccagta agtgcaacta taaatccagc tcctgctgaa attgttgctt
    17761 gtcttacagt tattctaaat cctgttggtc tacctaattt agtttggtca tcagttaaag
    17821 aatattgagt cttagccata catactggaa ctttagtaaa tccaagtttt tctaagttag
    17881 ctatttcctt atcagcttca ggagtataat ctacaccatc tgctccataa atcttagtag
    17941 ctatagcatt tatcttgtct tttatagata aatcatcttc atagcagaat ctaaagttat
    18001 tttcttcttc atccaataat cttaaaactt ctttagctac tgcaattcca ccttctccac
    18061 cttttgccca tacttcagaa agagcaacat taacacctaa ttccttacac ttgtcttcaa
    18121 caagtttaag ttcagcttca gtatcaagtg ggaatctgtt gattgcaaca actgctggta
    18181 atccatatac ttgagttatg ttttcaacat gttttaataa gtttggaagt cctttttcaa
    18241 gagcttctag gttttcgtta tttaagtctg ctttagcaac tccaccattg tattttaatg
    18301 ctcttacagt agcaactatt ataacagcat ctggttttaa gtttgccatt ctacatttta
    18361 tatctaagaa tttttctgct ccaaggtcag caccgaaacc agcctcagtt attacataat
    18421 ctgcaaagtg catagccatt cttgtagcta taacagagtt acatccatga gctatatttg
    18481 cgaatggtcc accgtgtaca aatgatggtg ttccttcaag agtttgaact aggtttggtt
    18541 ttaaagcatc ttttaataat gcagccatag caccattagc ttttaattgt ctagctgtta
    18601 caggttcacc ttcgtagctg tatccaacta ttatgtttcc taatctttct tttaaatcag
    18661 atatatcact tgccaaacag aacgctgcca ttatttcaga agcaacagtt atatcgaatc
    18721 catcttgtct aactgctcca tcaccttttt tacccatacc atcaacgata tttctaagtt
    18781 gtctatcgtt catgtctaca catcttctcc aagttatttt cttagggtct attctaagtg
    18841 catttccttg gtgaatatgg ttatcaagca tagcagctaa aagattgtta gcagctccta
    18901 tagcgtgaaa atctcctgtg aagtgtaagt ttatatcttc cataggaaca acttgtgcat
    18961 atcctccacc agctgctcca cctttaacac caaatactgg tcctagagat ggttctctta
    19021 aagcaactaa tacatttttg tctagtttag caaatgcatc agcaacacct atagtagtag
    19081 tagtttttcc ttcacctgct ggagttgggt ttatagctgt agttagtatt aacttagctt
    19141 ttttaccagt ctctcttttt agtagattgt agtcaacttt tgctttgtat ttaccataaa
    19201 gttctacatc atcttcacct agtcctaatt tttttgcaac ctctctaata tcttgaggct
    19261 tagcttcttg agctatttca atgtcagatt taaatcccat aactgaatct cctcccacaa
    19321 cctaaataga tttaaaatat aatttttgta aaaattgtat taatatgtaa aaattatatt
    19381 ttataagata attatacatg taaaattact taaaaactag ctactaagct attaaaatat
    19441 tcaaacttaa tagctagtta tttatctaat tacttactta ttacctatct attgcctatg
    19501 tctaaccttt aaccaactta aaatcccaaa tcaaaatttc tatctatttt atttatcctt
    19561 aagtgacatt aatttatctt ttattgcttt aatctgttct ttcgttgttt cactagaatc
    19621 atatggagat tgattagctc tatcagaatc aataacatct tggttataat aaataaatcc
    19681 taaagattct ccatcttcta agttttggat tataaattct tcatcttcct tatttctaat
    19741 tttatttcct actacaaata ctttatttac acctatatca tgtccaagtt tcttaacttt
    19801 tctataagtc tgtagacttc tctctcctgg ttctacaact actataaaag catctacacc
    19861 ttgggctgtt cctcttccta agtgttcaat tcccgcttcc atatccataa ccactacatc
    19921 tttattttgt agtattaaat gtgagcagag tctcttcaaa agaacatgct ctggacatac
    19981 acatccagta ccaccagaat ctacggttcc caatgtaagt aatcttactc cattgtattc
    20041 tttacagaaa ttttcaggga tatcgtctac tttaggattc atcttaaaca tttttccaaa
    20101 tgaacctaca gaagcagctg ttctatctga aactaatttt ttcatttctg atataggtac
    20161 tattgattca tatacttctt ttggaaaacc tagtgctaaa gctaaattag catcagggtc
    20221 ggcatcaaca gcaacaactc tgtatccgtc ttcagcaaac atcctagaga gcattgaaga
    20281 aaaagtcgtt ttacctactc cacctttacc tgttattgct atcttcataa tccctaccac
    20341 cttttattac tatttgcttt ttagtagtta attttacagt taaatatttt ttatactcta
    20401 tatttttata cactaaaaat aaactacagc tagtccttac aggaaccaat taaatttacc
    20461 ctactagctt ttcaaagcag catgtatagt tttattattc tttaatattg tttatatttt
    20521 atttattcag tatattcatt atattttgat gttctttact ttaatatttg actttattca
    20581 actttattca accagaaact tcttataaaa acgtttattc tataacttca ctatttttta
    20641 agcttcagcc ttttctgcca tcttttcttc aactaatgct tcaaagtgaa cacgtttttt
    20701 ctctatatct tctattattg tagctgctaa ctcttttgga tttgtattta cagtaaatgt
    20761 agctccaact ttatctttta attcactagt taatatatcc actgctcttg aagaaccagc
    20821 tattggaggc attattccta aataagtgtc tataccagaa gctacaacat agcatcctat
    20881 agcaactgct ttttctgaca tccactctgg agctatacct acaactggaa ggtcacacat
    20941 atccatatct aagtaattag ctgctgctcc aactaaatct agtatacgac ttatatcaac
    21001 acaagaaccc atatgtagta ctggtgggat tcctactaat ttacagacag ttgcaagacc
    21061 tttacctgca tatttttcag ccgcatcaga ttccattaaa ccaaacttag ctgctgctga
    21121 agctccacaa cctgtagtaa ctactattat gtcgtttttg attaattctt tcattattgt
    21181 tacgtgagcc tcatttgaaa ctcctttagc attgttacat ccaactacac cagctgctcc
    21241 tcttaatact ccagatttta aacagtctgt taatggctta actgttcctg ctgggtctat
    21301 atgagagttt acaactctgt ctaattgatt tactactgct tctacactat acccaactgt
    21361 tgcgcctgat tttaattcag gtataagtac ttttgattta tctctatttt taaagtttaa
    21421 aatagcttct ttaactattg ctttagcatc atcaagtgct tgttcttctc taaattcaat
    21481 ataagttgaa cctgttatct tagcttttgg agaagttgtt acaaatttag tatggtaaca
    21541 atctgcaact ctagctaatg cagggaatat acattgaacg tcaactatta cagcttcaac
    21601 tgctccagtt attattgcaa gttcttgttg atggaagtct cctgctattt ttacaccatg
    21661 tctcattgta atctcattac ctgtacaaca catacctgct aagtttattc catcagcacc
    21721 aacttcttta gctaaagcta ctaagtctgg ctcttctgat gctaaaacta tcatttctga
    21781 taatgctggc tcatgtccat gtaatattat gttaacttta ttttcttcta atactgctaa
    21841 attcgcctct gttcttcttg gcactggagt tccaaataag atatcgctta gacgagtacc
    21901 tatcatagaa ccaccccaac catcagccat agaagttcta agactcatgt gtattaaact
    21961 atctatatca ccagtacatc ctatatgagt agagtgcatt attgttgcaa tttctctatc
    22021 tatagctctt ggctctatag cgtattcttt ccatattttt tgtcttggtt ctggtgcatt
    22081 ttttataagt cttaatgtcc caaaaggttt tccaaattcc attaatgcaa tttctgccac
    22141 ttcatgtgct acatcataga tatctctacc ttctgtctct acatcccatt ctttagctaa
    22201 agtaattaac ttagcttcgt ctcttatttt atagtttcca tttggatcag ctaatgctaa
    22261 tgtatgtgct atatctctag catggtctga atgggcagct gtcccaccag caaccattct
    22321 tgcaaagttt ctacctacta tagtatgctc atctgctcca cataaccccc ttggagtctt
    22381 agggcttatt ctacatggtc ccattccaca tattctacag caaacacctt gaagaccaaa
    22441 accacattgt gccttaaagc cttcttttct atcatacatt gtttctacgc cttcttctct
    22501 agctttagct atcatcttct ggctgtttaa atcaattgtt aacatttttt catccatatt
    22561 cctatcccct ttttttgatt tttaaaatcc cccaggtgac tgacttcatg tcataaaata
    22621 ataaaataaa tgctttctaa gttgattata tactaatttt cagtgctttg ctttagattt
    22681 aaatattttt ttaacaaaat tagataatac ataaaaattt tattttctac acttaattta
    22741 agttgatttt atcgcttgtt tttggctatt tgttaatatc taaacctact tttttgatac
    22801 ttcagtttaa ttaaattatg ttttgatttt tttaaatcaa atttgaaatt tttctagtca
    22861 tttttatatt attgcagatt gtttgtgtat ttattatctt tcactactgt ttggacttat
    22921 tacagatttt tactccctaa acttaattag taaaaacttc ttagaatagc attttttgga
    22981 ctaatttttt tgaatttttt tatttttcct actttaatat taattatagc ttcaaattga
    23041 acatttgtat atattcattt gaagctataa ttatttttta tataaaactt ttttaataca
    23101 gcattaattt agacattttg actatttttt ctactttaat tcatttattt caagcgattt
    23161 atcctatttc atatttttag ctacaatagc cccatatgca atagaatata atttagattc
    23221 atgtgaatca gctcttgaca ctaaaactat aggagctttt gcacccataa ttatacctgc
    23281 tgatttagag tctgccatat atgtaaaggc tttccctatt ccattaccaa cctctattgt
    23341 tggaaccaat atgatatcta catctccaga tacttcactt ttaaaccctt ttatctcact
    23401 agcttctttt gaaacagcta aatcaaaagc cagaggacct tctacaatta cattttcacc
    23461 aaattctcct tctttacaag cttttgctaa catatctgca tctacagttg cttgcatctt
    23521 aggatttact ttctctttag ctgccaaaca tgcaactttt acagtttcca ttcctaaagc
    23581 ttttgcagct tttatagaat tttttaatat ctttgccttt tgttcataat caggagctat
    23641 attcatacct ccatctgtag ttataagtaa tttatggtat ttttctaact catataccat
    23701 aacatgactt aataaactat cagttctaag accatagtct ttatttaaca cttcttttaa
    23761 tatgacagat gtatctaata atcctttcat tacaaagtcg gcttctttac ttgatactaa
    23821 tttaactgct atttctgcac actctttgat atcatcctta tccactattt tgataccact
    23881 taaatcaaat cctatttctt ttgatatttc tcttattttg ccttcatttc caaccaatat
    23941 aggagtaatt atatttcttt ctacagcatc ctttattgcc attaaaactt ctttatcatg
    24001 agctgctgct acagagagta caattttctc tcctccctta agttgttcta aaatatcatc
    24061 taattttttt atcaaaagta acccctccct tatatatatt taactaaata ataccatatt
    24121 tgctatacct tttctcaatt tattttttat gttataaaga atttatataa ctaaaagaca
    24181 gtttaatatt ttattctggg atttcatcaa taactcttaa aagctttgca ggaacaccac
    24241 ctacaatagt attttctgca acattttttg ttacaactgc tccagcagcg ataattgcat
    24301 tatctccaat agtaactcct ggaactacag ttacattagc accaatccat acattttttc
    24361 ctattgtgat tggagaagga taagttgtac cacgtttctc tggtgcaata ccatggttaa
    24421 gtgtagccaa taccacatta tgcccaattt gcgtattatc tccaatctca atccccccct
    24481 ggtcttgaaa atggcaactg ctatttataa aaacattttt tcctattgtg atgttttttc
    24541 cacaatctgt ataaaatggt ggaaacataa aaaatgattc atctaccttt tttccaatta
    24601 actcagaaaa taaactacgg acttcttctg gttcatgata agaagaattt aacttacttg
    24661 tgattttcat tgcttcatta ctcaacttaa ccataaattc atgtatttct gatttactag
    24721 aaacaggttt tctttgattg atatgtgtta aaaattcatt gatattcatt ttatacatcc
    24781 tttcaatatg tgctttaatt tatttgtaca ttctaaataa tattttaata a
//
LOCUS       DAYAOX010000038        24682 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.038, whole genome shotgun sequence.
ACCESSION   DAYAOX010000038 DAYAOX010000000
VERSION     DAYAOX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.038"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            38..742
                     /locus_tag="ACGI57_001229"
     CDS             38..742
                     /locus_tag="ACGI57_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9716314.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            744..2231
                     /locus_tag="ACGI57_001230"
     CDS             744..2231
                     /locus_tag="ACGI57_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9716315.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            2436..4049
                     /locus_tag="ACGI57_001231"
     CDS             2436..4049
                     /locus_tag="ACGI57_001231"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9716316.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(4412..5242)
                     /locus_tag="ACGI57_001232"
     CDS             complement(4412..5242)
                     /locus_tag="ACGI57_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9716317.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            5315..7042
                     /locus_tag="ACGI57_001233"
     CDS             5315..7042
                     /locus_tag="ACGI57_001233"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716318.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            7079..8941
                     /locus_tag="ACGI57_001234"
     CDS             7079..8941
                     /locus_tag="ACGI57_001234"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716319.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            9274..9963
                     /locus_tag="ACGI57_001235"
     CDS             9274..9963
                     /locus_tag="ACGI57_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HFG9716320.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            10080..10754
                     /locus_tag="ACGI57_001236"
     CDS             10080..10754
                     /locus_tag="ACGI57_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFG9716321.1"
                     /translation="MTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMVYKKT
                     YMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDPMDLV
                     FLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNEDSEDI
                     ATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAVLVSY
                     NGDYVA"
     gene            10771..12678
                     /locus_tag="ACGI57_001237"
     CDS             10771..12678
                     /locus_tag="ACGI57_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HFG9716322.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            13131..13820
                     /locus_tag="ACGI57_001238"
     CDS             13131..13820
                     /locus_tag="ACGI57_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9716323.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            14256..14795
                     /locus_tag="ACGI57_001239"
     CDS             14256..14795
                     /locus_tag="ACGI57_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9716324.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            14814..15857
                     /gene="potA"
                     /locus_tag="ACGI57_001240"
     CDS             14814..15857
                     /gene="potA"
                     /locus_tag="ACGI57_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1902047 - polyamine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HFG9716325.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            15859..16695
                     /locus_tag="ACGI57_001241"
     CDS             15859..16695
                     /locus_tag="ACGI57_001241"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9716326.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            16800..17474
                     /locus_tag="ACGI57_001242"
     CDS             16800..17474
                     /locus_tag="ACGI57_001242"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9716327.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            17474..18526
                     /locus_tag="ACGI57_001243"
     CDS             17474..18526
                     /locus_tag="ACGI57_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9716328.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            18756..19604
                     /locus_tag="ACGI57_001244"
     CDS             18756..19604
                     /locus_tag="ACGI57_001244"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9716329.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            19594..21258
                     /locus_tag="ACGI57_001245"
     CDS             19594..21258
                     /locus_tag="ACGI57_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HFG9716330.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNLYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            21307..22563
                     /locus_tag="ACGI57_001246"
     CDS             21307..22563
                     /locus_tag="ACGI57_001246"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9716331.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            22586..24667
                     /locus_tag="ACGI57_001247"
     CDS             22586..24667
                     /locus_tag="ACGI57_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFG9716332.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIIVMIL
                     SMLYIKKYKRR"
BASE COUNT         9749 a         2442 c         4244 g         8247 t
ORIGIN      
        1 tttaatttaa ataaaaagtc tattaaggag tgagcttatg agtaagttaa tttatatagc
       61 agatgatgaa gataatataa gaaatttggt aaagacattc ttaaaaaatg aaggtcatga
      121 tgttatggat tttaaaacgg gtgatgaact tttagaacaa tttaatataa aggagtgtga
      181 cttagtaata ctagatataa tgatgcctgg ctcaagtggt tttgaagtat gtacaaaact
      241 tcgtgagaaa agtacagtcc caataatcat gcttactgca agagatacag atatagatta
      301 tattacaggg ataaccttag gtagtgatga ttattttaca aaaccattta gcccaatgtc
      361 tttggttatg agagtaaaat ctatatttag aaggatagag tttgaaaaga aacaaaatta
      421 tgataaatat agtaactcga tagatatgga attaaaattt ggtgatgtaa taataaataa
      481 aaagaataaa atcgttacaa gtaaaaatgt aaatatagac cttacaccaa atgaatataa
      541 cctattgact tatttgtttg aaaatataga tagagcagtt tccagagatg aattattaaa
      601 caaaatatgg ggatacgata tagaagttga gacaagggct gctgatgata cagtgaagag
      661 attacgtaaa aaaattttaa atacaaatat tttaatagaa actgtatggg ggtttggatt
      721 taggttgaag gagaaaagtt aagatgaaga atggaaatga caagaataga attggaaaga
      781 ttaaatccaa atttaaatta aagttctcaa agaaaaatat aagcataaaa actaggattt
      841 tgttgagtat actttgtttg ataattatag tatttatgat aatattagta tcttttaatg
      901 tatttcttga tacatatata aaaacaactg cgaatgaaga attgacaaag tctacagaaa
      961 tagtagaaca tatggatagt aattttagac ctattaagcc accacaagat gatggaaagc
     1021 ttcctcccaa agtattatct aattttatga gaaatgtaca agataaagtt aggatggcag
     1081 aaacacaatc agatgctgat gctatggtag ttgattctaa gtataacctg atatttccaa
     1141 caagagagga tgatttctta aaaaatgttg atgagatgga gctgattagg gagtgtattc
     1201 aaaatgaaaa attagattta aattcagatg aaaatacacg gatatctacc agaaataagg
     1261 attattatat ttcaactgta aaaattacta gtatatatgg tgaacaagac aagtatctta
     1321 tactttttat agacatatca aaaactctaa atctagcaca aaagataaat attgtattaa
     1381 tatctgtaat gtgttttgct ggaatattag caatatttac agctgtgatt ttatctgaga
     1441 aaattgctaa accgataaaa gagttgtgtg agtttgcaaa aaaaatgggt caaggagatt
     1501 tcaaaagaac ctactttgac ttttcagata aagaattagt agagctctca actgttatga
     1561 ataagagtgc tgagtatctg gataagtatg ataatgaaca aaaaatattt tttcaaaatg
     1621 catctcatga attaaggaca ccacttatgt ctattaaagg ttatgcagaa gctataaaat
     1681 ataatgttat agatagtaaa catgcaagtg atataattct tgaagaaagt gataggttaa
     1741 gtgatatggt tgaagattta ttatacattt ctaaaataga taatataact aaagattttg
     1801 aacttgtaga atgtgattta agagaagtac tttcaaattg tggagctagg caaaatgtaa
     1861 gagctataaa taaggggata aaatttattt atgaatttga tgaagaaatg gttttatttg
     1921 aatgtgatga aaaaaacatt tccaaagcat ttatgaatct tatagaaaat gctttgagat
     1981 acgctaagag tgaaataaaa atagtgtgta agtataatca aaaaaatata gttgtgataa
     2041 tagaagatga tggaattgga atcaagaaag aagatttacc tcatgttttt gaaagattct
     2101 ataagggagt agggggaaat catggaattg gattatcaat agttaaatct attgtaaata
     2161 agcatggtgg gcgtatatac gtagaaaatg gcaaaaaagg agctaaattt actatattat
     2221 ttaaattgta aaaaaattag agaaaatata gagatgtttg aagttgtttt ttatatatat
     2281 ctgttgtata ctgttgaaaa aagaacaata ttaagctgac atatattacg atagttgaca
     2341 tttaaagtaa atgcttaaat atagatacga aaggaaaagt aaaatctgaa gtgtatttag
     2401 gtttaagtaa gcttcttatt ttataaaaaa tatgtatgaa agaacagctg atttttaata
     2461 ttacaaattt agtaacggtt gtatttgaag cttttgtcat ccatatgttt ttatcagact
     2521 ttttaggtca gaaagaaggg tatataaaag ttgtaagata tgctaaactg ggttttataa
     2581 tatgtttagg attctgtaat ttaataactt taaaccctaa aatcacaatg cctttaattt
     2641 tcatccttat tttttcaaca tcatttttat ataaaggaaa tttaaagaca cgattgttta
     2701 ctactgtttt attaagtata ttctttatac tgtcagaaat tgtagttaca agtatttttg
     2761 ttttatttgt aaaagaaggc tttgaaataa tgcttgaaaa taatagtata agagtattgg
     2821 cgacaatttt atctaaaatt gtctttttgt tgacttgtaa aattatttgt ctatttaaaa
     2881 aggatgtaca tttagatatg cccataaagt attggctccc tttattttta atacctatat
     2941 ttagtttatt tctgtctgtt tctatctttg atgtaagtaa attttttagt cttgaaagtt
     3001 taaaatttct aagcttaata tctagtgttg gtattttata tattaatttt attgtctttt
     3061 atttatttaa atttattata gataaaacta aactatctat gaaatatgaa ctacttgaaa
     3121 aagaaataat tcataaggaa gaattaagac ttagtaatga gtgttataaa attattgttg
     3181 aacagactga ttctgtcgtt ttcgaatgga atatcaaaga aaataaatca tttgtatcgc
     3241 aggcttggac tgaaaaattt ggatataaca atgcatgtaa gaatattttt aaagaaatta
     3301 aagataaaga cttagttcat tctgaagata aagctatatt tgaaggtttt cttgaaagta
     3361 ttaaaaaaaa gaatatgcac aatcaagcag tatatcgttt aaaaaaatct aatggtgaat
     3421 atatttggtg tagaacatca attacaagca tttataatga tgaaaatgaa cttttaagag
     3481 ttgtcggagt aattgtagat gtagatagtg atataaaaaa atatgaagag ttaagaacta
     3541 gagcagaaag tgactcattg accaatatat acaataaagg tacatttgaa aaattagtgg
     3601 aagaaactat agttatgaat acaggtgata aaaaagatgc attattcatt attgatttgg
     3661 atgattttaa agaaataaat gataattttg gtcatccatt tggtgatttt gtactaaaaa
     3721 catttgctga taagatacaa actagttttg gtagtaaaga cttagtcgga agaattggtg
     3781 gagatgaatt tgttgtatat atgcaagatt atgttacaga agtaaatttg cataaaaaag
     3841 ctaaagaatt aaatagagta ctttcggata attacactga tttgagtttt agttttgatg
     3901 cttctgtcag tataggtatt gcaaggtatc ctcaagatgg aacttcattt tttgaattgt
     3961 ttaaaaatgc agatagagca ctttatagca taaaagcatc aggtaaaaat tcatattgtt
     4021 tatttgaaga agaactatat gtacagtgaa tttgaattta gattttatgt gagagtaaga
     4081 tttatatata cttgtggctg tctgatttat aaaatatgtt actttgtata tttttatatg
     4141 tttcagtaag gttaattaac atgagcaaag gtgtaagtaa atttatatta gtttaagttt
     4201 acttacacct ttataaaaat acaaataata gattcattta cattcttaaa tgattaaagc
     4261 tctttacctt ctaaacatgc ataacatatc gttataattc cagtaaataa gactaaaatg
     4321 cccattacta agattacaat ttcatcatct attactccaa atgtttttaa agtaacttat
     4381 ttcgctttgt atcataataa tttgtctctt tttacatttt aacttctgct gagatttgag
     4441 ttagatattc attagaatct cgcacactta tatcatatag aagatattct tcataaacaa
     4501 aatccccaat acgtaaatta ttctgattaa aaaaatttaa taacttttca taagccttgt
     4561 aaatagtttc ataactccct ttatgataag cagttacata aagtcccttt ggtttgattg
     4621 atacaggata tttcttgtac tctttattaa cttttgtaaa taaatatgaa tatgattcaa
     4681 agtctttatt aattatatct tcacctttaa tgattgagcc aatcgtacca taatcactca
     4741 attcatagtt gtccataaag ttaatacatt catataaaaa attttcttcc tcatctccta
     4801 aaaactgttc tttatatgta acaggtgttt tgaccaagta ctcttcttca tgttctttta
     4861 aagtaatagt attcgcatct atattacatg cacttttagt aaatgaaata gtagattcca
     4921 ttagagcttg aatattattt agtttttcaa tttcattttt aatatcaatg gttttctttt
     4981 ctaaaagctc aattagtaaa ttaggtgttc ttttgtctat ataagcttta atttctttta
     5041 aaggcatttt tacttcccta agtgaagaaa ttataccaaa aatgctaagt tgatggtatg
     5101 agtagtatct atatccattt tctttcttta attctggtga gaaaagacct atatcatcat
     5161 aatggaaaag tgttttttta ttgattccac aaatctttgc aaattctcca gttgtaaaat
     5221 aattttttga aacttgactc ataattataa ttcctccttg actatcctgt tacagtatag
     5281 tttatcatag tattttgtaa aggagtgtga ttatatgaaa aaattaattc attttttaaa
     5341 accatataga gttttaattg ttgtagtttt gatatttacg tttttacaaa cacttggaac
     5401 tttgtatata ccaacactta cagcaaatat agtaaataat ggtgtagtaa agggagatat
     5461 agattatatt gtaaaaacag gtttaatgat gatgatagta gctggtataa cagctttatc
     5521 agctgtgtta gtttgtaaag tatcagcaaa tctttcatct ggattttgta gagatattag
     5581 agaagcagta tttataaaat cacaagattt atcaataaat gattttaata atataggtac
     5641 agcatcaatg attactagga gtactagtga tattacttta ataggtcagt ctgtgtttat
     5701 gtttattcaa ttagtattac cagcaccaat tataactgtt tctggtctat tcttagcata
     5761 ttctatagat aaagcaatga caattattat tgttgtagta atgtttttat tcatgctttc
     5821 agcattttta gttggtaaaa agcttataaa gttgtttaaa atgatgcaaa taaagatgga
     5881 taatatgaat agggtgttac gtgaggtagt aactggagta agagttataa gagcttttaa
     5941 taggagtcgt tttgaaaaaa agagatttga tagaactgca atagattatt ctgaaacagc
     6001 aatttcaata aataaaatat ttgcagtact tatgcctatt gtaatgttaa taatgaattt
     6061 aggtatagta agtattattt ggtttggtgg gatgagagtc agtaatggaa atatggaaat
     6121 aggtcatata atggcacttg ttgaatattg tatcttaatt ttattctatt taatcatggg
     6181 agtaatggta tttatgtaca tacctagagc aggtgcatgt gcagatagag taaatcagat
     6241 attagatata gaacctgaaa ttgtagatgg aaatggtcat aaagatactg tctcagagag
     6301 aggacattta gtatttaaaa atgtaacctt ttcatatgca caatcagaag aacctgtgtt
     6361 aaataatata acatttgaag caaaatctgg agaagtaact gcaattatag gaagcactgg
     6421 ctcaggcaaa tctactattg caaatataat accacgtttt tttgaaattc aatctggaga
     6481 aatatctata aatggtcagg atattaagaa gattcctcaa aaagaactta gagataagat
     6541 tggatttgta ccacaaaaag cttttttatt tagtggaaca atagaagaaa atataaggta
     6601 tggaaaagaa gatgcaagta ttgaagaagt aaagcatgct gcaagtatag ctcaagctga
     6661 tgaatttatt tatgatatgg aagacaagta tgactcattt gtagcacaag gtggaaataa
     6721 tttgtcaggt ggtcaaaaac agagaatatc cattgcaaga gcgttagtta gaaagcctga
     6781 agtatatgtc tttgatgata gtttttctgc attagatttt aaaacagata aaagacttag
     6841 aaaagcgttg aaaaatgaaa tcaaagattc atcagctata attatagcac aacgtataag
     6901 tacaatcatg gatgctaatc agattatagt tttaaatgat ggaaaaattg taggtattgg
     6961 aaaacataaa gatttacttg aaaattgtga agtttataag caaattgcgg attcacaact
     7021 tagtaaagaa gaacttgcat aaaatgaaga attaatctga tatttggagg tgtatgatat
     7081 gagtgaaaaa agaatagatg aacaagatat agaagtactt agttttgaag atggcatgtc
     7141 agcagaaaaa gctaaggatg caaaaaaaac tactaagaga ttattaaaat atatggcaaa
     7201 acaaaagttt aaaatattaa ttatttttat ttcagtattg ataagttcag cgcttacagt
     7261 tttagcacct atggttatgg gaaaagctat tgaccaacta tttaatggaa taaaaactgc
     7321 agtccaaaca ggtacaaaat tcagtgtgaa ttttagtact atgggaggga tagtatcaat
     7381 acttctaggg ttgtacttaa ttagtgcagt atttatatat attcaacagt atattatgtc
     7441 aggagttgca caaaatctag ttcttagcat gagaaaagat ttaagtgata aactcaataa
     7501 acttcctctt aagtactatg attctcataa aaagggggaa acacttagta tagtaacaaa
     7561 tgatttagaa aaagtagcag atagtttaca agaaggatta atgcaactta ttacagcagt
     7621 agttacagta gttggttcta ttgtaatgat gataagtata agtgtaccac ttacaatagt
     7681 atcagctata acattacttg tttcattggg tattactgtt gtaattgcaa gaaaatcaca
     7741 agaaagattc tctgaaaatc aaaaggcact aggagagtta aatagcaata tagaagagat
     7801 atttactggt caaatagtgg ttaaagcatt tagtaaagaa aaggatacta taagaaactt
     7861 taaaatatta aatcaaaatc tttataatgc aagtagaaaa gcacagtttt cgtcatatgc
     7921 aatatctcca attattagat ttataaacct aattggctat gttattatag cagttgtagg
     7981 tgggatattt gcatcaactg gagcaatgac tcttggtagt atacaagcgt ttattcaata
     8041 tgtaaatcaa gcatcagagc ctactacaga aatatcttat attgtaaata tgcttcaagc
     8101 agcaatagca tctgcagaga gagtttttac agttatggac gaggtggaag agataaagga
     8161 tgaagaatct tcaaaggtta ttagcatgcc taaaggtaaa gtacaatttg agcatgttaa
     8221 atttggatat agtgatgatt ttattcttat gaaagatatt aatatcaatc taaatgcagg
     8281 agataagatt gcaattgttg gacctacagg agctggaaag acaactcttg taaatttact
     8341 tatgagattt tatgagatac aaggtggaag aattactatt gatggtgtta atataaaaga
     8401 tttaaagcgt ggagaactta gaactatgtt tggtatggtt cttcaagata catggctgtt
     8461 taatggaagt ataaaagaaa atattgcata tagtaagtca gatgccacaa tggatgaaat
     8521 tgtaagagca gcaaaatcag caagagtaga ccattttata aggactttac cacaagggta
     8581 tgacactata ttgactgagg atgcttctaa tctatctcaa ggtcaaaagc aacttctaac
     8641 aattgccagg gcaatacttt cagacccatc agtattgatt cttgatgaag caacatctag
     8701 tgtagatact agaacagagg tagaaattca aaaggcaatg aataacttga tgaagggaag
     8761 aactagtttt gttattgcac atagattatc aactattcga gatgctgatt taatattagt
     8821 aatgaaagaa ggaactatta ttgaacaagg aagccataag gaacttattg aaaagaaagg
     8881 attctatgaa gaactttata acagtcaatt tacaagtgaa tatgatgaag atgttgtatg
     8941 attatcaagt tttaggcaac taaatgattg ataaatatta agttaaatat agtaacatgt
     9001 aaggttatat ggaataagag tatttctcta tttcatataa ccttttttat ttatcaaaat
     9061 aagataaaat ttcctttcta aattagattg aagaatgtat ttatcaatat aaaagtaaaa
     9121 taatcattat tattagtgaa gtaaatattg tttgccacat atatgccata aagatgcact
     9181 tattttacgg agatttaaca aagtagtgta aagtatactc taaatataaa ataaatcaaa
     9241 tagagttaaa taaattgaga ggtgatataa atagtgaata aaaaattgaa ctatagattt
     9301 gaaatgaagc acaaaataac tgaagcagat gtgttagctt taaaatcaag actctatcca
     9361 ataatgaaga aagatgaaaa tgcatctaaa gatggaaaat atcttattag gagtttatat
     9421 tttgatactc ctgaagataa agcacttttg gataaattaa atggcgttgc aataagagaa
     9481 aagtttagga taagatttta taataatgac tgctcttata taaggttaga gaaaaaaatc
     9541 aaacattaca acatgacatc aaagctatca actaatttaa ccaaaaaaga agtaataaat
     9601 atactaaata atgatattga atttctaaaa gagtcgacaa attgtttatt aagagaattt
     9661 tatttgaaat taaaatgtga aagattagag gcaaaaagca tagttgatta caatagagaa
     9721 gcttttattt atccagttgg aaatgtaaga gttacaatcg atagtgatat taaaacttct
     9781 gtaaattcgg tagatttatt taataaagat ttgccaacta tttcggtaat tgatgagaat
     9841 atgactgtat tggaggttaa atatgatgaa tttataccag attttataaa ggatttaatt
     9901 caaattaata agactacctc tacagcagtt tcaaagtatg cttcaagtag attatatatt
     9961 taagaataaa ggctatgatt tatataaaga tatacataaa taaggttaaa aaacaaataa
    10021 gttaaaaata aataaaataa aaaataaata agttaaaaat aggaaggatg attttaatta
    10081 tgacatttaa tgatattttt aaatcaagtt ttatagaaaa cgtatcaggt ttttcatttg
    10141 tggacagtgc tttagcacta ggttcagctt ttttagtagg tttgtttata tatatggtct
    10201 ataaaaagac ttatatggga attatgtatt ctcgaccatt caatgtgtcg cttgtagcac
    10261 ttacaatgct tacaactttt gtaatattgg cggttacttc aaatgtagtt ttatccttag
    10321 gtatggttgg tgctttatct atagtaagat ttagaacagc aattaaagac ccaatggatt
    10381 tagtatttct attttggtca ttaggttcag gtatagtttt aggagcaggt ttgattccct
    10441 tagctgtcat gggttcaata attatgggat tgattctaat attcttttca aataaaacaa
    10501 tatctgaaac tccatatata ttgatggtaa attgtaataa tgaagattca gaagatatag
    10561 caactgataa aattaaaaaa gtatttacta aatatcaaat aaaatcaaaa agtgttacac
    10621 cagaaaaagg aatagaatta gtgtttgaag taagaatgaa agatggagaa acaagtctta
    10681 taaatgactt gagtcaagta gatggagtta ctaatgcagt tcttgtaagt tataatggtg
    10741 actatgtagc atagagagga gtaatctttt atgaaagata aaaaatttac ccttcttatc
    10801 tcgattatga ttatattttt atgtgctgta gttggagttt atagtacatc tagcaacaaa
    10861 agtgctgatt tatatagtga tgtatatatt gaaaaatatt ttaacagaga caaggttatg
    10921 gaagttaata tagagataga tgaaagtgac ttgaaggata tgaatgaaaa tgctataaaa
    10981 gaagaattta aggttgcaaa agtaactgta gatggagata catatggaaa cgtaggtata
    11041 agaactaaag gaaattcaag tcttatatct gtagcaaata gtgatagtga tagatacagc
    11101 tataagatta attttgataa gtataatact agtcaaagta tggaagggct tactcaatta
    11161 aatcttaata actgttactc tgacccatct tatatgagag agtttttaac atatagtatt
    11221 tgcgaggaaa tgggattagc gactccagaa tttgcatatg ctaaagtctc tataaatggc
    11281 gaatatcatg gtttgtattt ggcagtagaa ggattaaaag agtcttatct tgaaaataat
    11341 tttggtaatg taactggaga cttatataag tcagatgaag gaagctcgtt gcaatataaa
    11401 ggagatgacc cagaaagtta ctcaaactta atcgttgaaa gtgataaaaa gacagctgat
    11461 tggtctaaaa tcacaaaact attaaaatct ttggatacag gtgaagatat tgaaaaatat
    11521 cttgatgtag attctgtcct taaaaatata gcaataaata cagctttatt aaaccttgat
    11581 agctatcaag ggagttttgc ccataactat tatttatatg agcaagatgg agtattttct
    11641 atgttaccat gggattttaa tatgtcattt ggtggattta gtggttttgg tggaggtagt
    11701 caatctatag caattgatga acctacgaca ggtaatttag aagacagacc tctcatatcc
    11761 tcgttattaa aaaatgagac atacaaaaca aaataccata aatatctgga agagatagta
    11821 acaaaatacc tagattcaga ctatttagag aatatgacaa caaaattgca tgacatgata
    11881 gcatcatatg taaaagaaga cccaacagca ttttatactt atgaagaatt tgaaaaaaat
    11941 ataacatctt caattgaaga ttctagtgat aataagggat ttggtaataa agggtttgac
    12001 aacaataact ctaataacag tgattctaat aataattcta atagtgaaaa taagcgctct
    12061 ggaaatcaaa gtgatgaaaa agaagttaat gctgaattaa catcaagcgt agtcaaagct
    12121 aatacagata atgaaactaa aaataaaact acaaatgata gtgaaagtaa gaataataca
    12181 gataaagata aaagtggaaa tgataataat caaaagctag aaggtcctat gggtaaagga
    12241 ggtaagtcaa taccaggggt tttggaagtt gcagaagata tgagtaaaac tataaaatct
    12301 caattaagtg gagaaacttc ttcgacaaag caaaactctg gtgatgaaag ttcaagtgga
    12361 attaaaggta gtgaaaagtt tgatgaggat atgagtggta tgccagaacc acctgaggga
    12421 atggatggta aaatgccacc aggaatgggt aatatggata agggagatat gaatggtaaa
    12481 aatggcaata tgaatatgga tagaaatcaa gataatccaa gagaagctgg aggttttggc
    12541 aatagaggag gaggctctgt gagtaaaaca acaacatact tcaaattaat tttaggtgga
    12601 gcttcaatga taataatgtc gattatgtta gttggtgtat caagggtaaa gagaagaaga
    12661 tttataaagt caaaataaga ttaaaattga atataaataa ttttaatact gttaagtcta
    12721 aatatttact tagcagtatt tttttagaaa ataatgtatt tggttaataa tataaggtaa
    12781 taaattgata aaaattaaat acagcatatt aataatacat aataaagtaa taaaaaaact
    12841 gtttaagtat aataacttat atttagacag tttttttcat ttataaataa tataaatgaa
    12901 attgaaaaca tttgaaggat agaaattggt tggataaatg tataaattac ttcaccaaaa
    12961 aataatgaca aaaattgtaa ctaagttgta ataaaatggt agttatttag cataaaataa
    13021 tgtatcatag aagtatatta taaaattaat aaaataagat ttttgtaaga aaactgctgg
    13081 cttttgatat ttagagtgag tagttaaatt atgtttagga ggggtagaat ttgttgcaaa
    13141 aagtattatg gaaattttta aagctagtag tcggtttatt tatatgttca gttggtatag
    13201 tattgacgat aaattgtaac cttggattat ctccctggga tgtatttcat caaggacttt
    13261 caaatcatat aggaataact ataggaactg cttctataat tgtaggctct atagtagtta
    13321 ttgctgatgt cgttcttgga gaaaatgtag gatggggaac tgtttttaac atgctactaa
    13381 ttggattctt tatggattta cttttatata gtaatttaat tccagaggct gatagtttat
    13441 ttgtaggaat aattatgttg atattaggat tggttctttt gtctgttgga atggtatttt
    13501 atatgggaag tggtttagga agtggaccta gagatggttt gatggttgca attcaaaaga
    13561 aaacaggaaa atcacttaaa ttaataagag gtactataga agttggagca ttaatagtag
    13621 gatttttatt aggtggaaaa gttggtatag gaactataat aagtgcattt ggattggggt
    13681 atttcacaca aatggtattt ggcttattta aattagattg tagtaagatt aaacatagat
    13741 ttatagtaga tgatataaaa tttatcaaag tgtatatgag tggtgagaaa aaagctgctg
    13801 ttcaaaatat agttaagtaa tttaagaata aattcaacga aatgttaagt attttaaatt
    13861 aacattttgt ttagatagat aaattgaata ttaaaagaat attaaaatat gaggattgct
    13921 agaacactat aaaatataga tttctagcaa ttttttattt gttcaatatt tttttagtac
    13981 tagaagtctt attctctatt taatgaatga attaatttat aattagagat actaaacttt
    14041 atagatatta atataaacac ctttaaactg tgtattttcc aaagccttta aaatgcttgt
    14101 atttattcta aataaaaaaa taaaaaaatg tcaaaaaaac tcttgtatta tatccaaatt
    14161 ttatatataa ttacctatgt tgtttagaaa ttttaaactc aatgtttagt aaacaaaatg
    14221 tttagtaaaa taaaacagaa actaggaggt gcaacatgga tataggtgaa aagataaaaa
    14281 gattaagaac agaaaaacaa ctgacccagg aagagttagc taatagatgt gaattgtcaa
    14341 agggatttat ttctcaatta gaaaataact taacatcacc atccatagct acccttatag
    14401 atatacttga aatactaggt acaaatttaa gggaattttt taatgaaata gatgatgaaa
    14461 gaatcagttt tacaaaagaa gatatgtttg aaacagaaga tgaagattta aaatataaat
    14521 taaagtggtt aattccaaat tcacaaaaaa atgaaatgga gccaataata ataacattgt
    14581 atccaggagg acagtataaa gaagaaaagc cacatgaagg tgaagaattt ggatatgtat
    14641 tagcaggttc aatatatgtc catataggcg aaaagaaaaa taaagtaaaa aaaggagaga
    14701 gtttttactt tagaccgaaa gcaaatcact atatatcaaa cgagggcaaa actacagcaa
    14761 aagtgatttg ggtaagtacg ccaccatcat tttagaatag aggtgtttaa taattggtag
    14821 aaaatataat agaattaagg gatatatcta aacattatga agaacttact gttttagata
    14881 atttcaattt agatataaag aaaaatgagt ttctaacatt acttggacca agtggatgtg
    14941 gaaaaactac aacattaaaa ataatagcag gatttgagta tgctgatgat ggtaaagttt
    15001 tatttgaagg taaggagata aataatttgc caccatatga aagacaagta aacacagtat
    15061 ttcaaaaata tgctttattt ccacacatgg atgtttatga aaatgtagct tttggattaa
    15121 aaattaaaaa gactccaaaa gatgtaatag atgctaaagt aaaagaggtt ttgaaacttg
    15181 ttgcactaga aggttttgaa agacgacaag ttgaatcatt gagtggagga caacaacaaa
    15241 gaattgcaat agcaagagct ctagttaatg aaccaaaggt attattacta gatgaaccac
    15301 ttggagcact tgatttaaag ttaagacaag agatgcaaat agaattaaag agaatccaaa
    15361 aaaaattagg aataacattt gtttttgtaa ctcatgacca agaagaggct cttactatgt
    15421 cagatactat tgttgtaatg tataagggca aaattcaaca aatgggaaca ccacaagata
    15481 tatacaatga acctaaaaat tcatttgtag ctaagtttat aggtgaaagt aacatatttg
    15541 atggaattat gatagaagat tataaagtta atttctgtaa tagagatttt gagtgtgttg
    15601 ataaaggatt cgaaaaaaac gaaaatatag aagtagtaat aagaccagaa gatataaaaa
    15661 tggtaaagcc agaagaagga atgttaaagg ggaaagttac atctacagtg tttaaaggag
    15721 tgcattacga gatagaattg aatgaaaatg gaagattgtg gatacttcat aatactaaaa
    15781 atgctgaggt tgggactgaa ctaggcatgg atatatatcc agaagatatc catataatga
    15841 gaaaagagaa taactaagat gaaaagaaaa tcttttttag catatccata tgtagtttgg
    15901 agtgcgatat ttgtaataat tcctttaata ttagtagtat tttttagttt tacaaaagaa
    15961 tcaggtggag gttatgcctt tacacttgag aattataaag aagtaattga ccctatatat
    16021 atgaaagttt ttgggcgttc tattctttta gcaggaggag ctacattaat atgccttata
    16081 gttggttatc cagtggcata tataatttca aaggctcgtg taagtagaag aggttcatta
    16141 atattattgt ttattcttcc tatgtggatg aactttcttt taagaacata tgcatgggtt
    16201 gctatattag gaaaaaatgg acttttaaat acatttttag gttggtttgg aatacaacca
    16261 ttagcaattt tgtatactaa ttttgccata cttttaggta tggtgtataa ctttttacca
    16321 tttatggttc ttccaatata tacagcacta tcaaagatgg ataatgattt gataaacgct
    16381 gctcatgact tgggtgcaaa taatatgaca gtttttagaa aaattatatt tcctttgagt
    16441 ttgccaggtg taatgtctgg aataactatg gtatttatgc cagcagtcac tacttttgca
    16501 atatcaagac tgttaggtgg aggtaaaata atgctggttg gagatttgat tgaacaacag
    16561 tttacagttg ttggagattg gaactttggt tcagctatat cgatatttat gatgatagta
    16621 atattaatat ctatgtctat aatgtcaaaa tttggagatg aatctgacaa agaaggcggt
    16681 gggttactat tttaaaatta aagaaaataa cttctaatat atatttagca ttagtattct
    16741 tatttttgta tgcacccata tttgctttgg tactattttc tttcaatgat tcaaaatcaa
    16801 tggcaagatg gaatggattt acatggaagt ggtatggtca attacttcaa aatgagagta
    16861 taatgagtgc tttatattat actattgtaa tagctatatt agcgtctgta atatctacaa
    16921 tagtaggaac tataagtgct ataggaatac ataagatgag aggtaagagt aagaaattaa
    16981 tactaaatgt aaactatctt cctattttaa atacagagat agttacagca gtagctctta
    17041 tgagtctgtt tgtatttgta aaaatggaat ttggatttac aacaatgcta ttggcacata
    17101 taatgttttg tttgccatac gttatacttt cagttttgcc aaaaatgaag caattacctg
    17161 ataatataga agatgcagct atggacttgg gagcaacacc tatatacgct cttagaaaag
    17221 taattttacc acaaataaaa ccaggtatag tttctggatt tttaattgca tttactatgt
    17281 caatagatga ctttataata agtttcttta atgctggaaa tggtgtaagc aatctttcta
    17341 tagaaatcta tggaatggct agaagaggta taaaacctga aataaatgca ttatcaacaa
    17401 taatgtttgc agtggttcta ggtttacttt tactagctaa taaaaaagaa tctatagtaa
    17461 gggggattaa ataatgatta agtttaaaaa agcaatatcc cttatagctg taggtatgat
    17521 gacagttact ttatttacag gatgtaataa agcaactgat tctacaaaag ttttaaatgt
    17581 atataatgtt ggagattata tagatgagtc tcttatagat aagtttgaaa aagaaacagg
    17641 aatagatgtt caatattcaa cttatgatac taatgaaatg atgtatcaaa aagtaaagag
    17701 tggtagtaca cactatgatt tagtatttcc ttcagactat atggtagaga aaatgaaaaa
    17761 tgaaggtctt ttggaaaaat tagattttaa gaatatacct aatatgaaat atttagataa
    17821 gagtttttta aatcctgtat acgacaaaac taatgaatat agtgttgcat atatgtgggg
    17881 aactcttgga atactgtaca ataagaaaga agttaaagac cctatggata gctggaatat
    17941 actttggaat ccaaagtata agggaaatat aatgatgttt gactctgtta gagatactat
    18001 agggataact cttaaaaaat taggatatag catgaatagt gtaaatccaa aagaaataaa
    18061 tgaagctaaa aatcttttaa tgaaacaaaa ggatttagta ttggcttatg taaatgatga
    18121 aggtaaggat agattgcttg gtggagaagt ggcaatgggt atgctgtatt ctggagatgc
    18181 agttacacta atggagcaaa atcctgacct tgattatgct atacctaaag agggaactaa
    18241 taaatgggta gatgctatgt gtgtacctac aacagcacaa aataagaaag aagctgagct
    18301 gtttataaac tttttattag acccagagaa tgcaaaagta aatgctgagt acattgggta
    18361 ttcaacacct aatacaggag cactaaaatt attagaccca gaaataactc agaatcctgt
    18421 tgcatatcca tctaaagaag tgcttgataa atgtgaaact tttgaagata ttggtgaaaa
    18481 tataaaatta tatgacaagg cttggataga gttaaaatca aaatagggtc ttgttggttt
    18541 aagtaaattt acttaataaa tacttaataa aactgttctt ttttagaaaa tttattttct
    18601 aattattgaa cagttttatt ttttgtttaa gttatattca tatgcaattt aaatagttta
    18661 ttaataaatg aaaacttatt tgtataatga ctttaataat aattagaaaa aatgggcaac
    18721 ttaatagaat aattggaggt gtaaatttat aaaaaatgaa taggataaat aaaaaaatag
    18781 atttattaat attggctttg ctcgttttta tatttttaat tgttggaata gtaatactat
    18841 cgattaggac agaaaataaa ttgaatgtgt tcataatgtt agcaataatt ttttttataa
    18901 taatgttaac ttatttatca aattcagtag tggggttgat aacaagttca ataattatat
    18961 ttatgtatac atcctatatt ttatataaca atattactca caatatggat gttgaattta
    19021 tatcatatat gtggataata gcaacaccag tatcttcaat tataatgggt aatctgaaca
    19081 aaagcataaa tgaattgcaa aatacaaata aaaaactatc tgaacagtat aaggaattag
    19141 tgactattga tagtgaaact ggacttagaa atttaaagat tttttataat gatgtaaata
    19201 tggagataag taaatctatt agacataata ctgatttttc tcttatgata gtaaaattac
    19261 catactacgg aaacctacag actatatttg gtgaaaacaa aactaataaa atagtaaaat
    19321 atattggttc aaatattata gaatgtacaa gaaatgaaga tataatttat agtttacaaa
    19381 aagatatgat agggatacta atgccaaata catctttgga aggttcaaaa gttgttaaag
    19441 acagaataaa gaaaaggata aaagaattaa atttagattt aaacaatagg ggaaaatatg
    19501 taaatataga tgtcaaaatt gcatttttac aatataagag tagttttgga gatagtataa
    19561 attttaagaa tatagttgaa gaggaacttc aatatgatgt ttaaaatatt taataaaagt
    19621 ctattaaaat tagtagttgt attatcaata gtactgacaa tatcaaattt tagcttatct
    19681 tcatatgcat cagaaattac ttccagcaca gataaggtat taatcatata tgattctaag
    19741 aaagaaacag cttataatag agatatttta aatattatga ggactctatt gggaagattt
    19801 agttcagata tagagctatt aaaattgagt aactatgatg gtgagataaa taaaaattat
    19861 tacagtcata tatttatttt aggaatcaat gaaaactcat ataataatga taaaaacact
    19921 aaaaacttga ttagttcctt aaatttatat aaaggaacta tatgttggtt aggatatggt
    19981 atagaaaatc ttttggaaca taaaaaatac aatttagact atgtaggaaa aacaaacaat
    20041 atagttagtg taaattatag aggtaaatca tataacttgg atgaacatta tgtttttaac
    20101 attgtagaat caaaagatac aagtaataaa gtaataggaa gtattaatga taccttaaat
    20161 aagtatccat atataataaa tgataaaaat cttttttatg tatcaaaatt agatttagat
    20221 ggagttttat tttatatatt ttgcgattca cttaatgata tttttaatat taagacgttt
    20281 gataaaggac gtatatttgt aagaatagaa gatgtacatg cttttagaga accaaaaaat
    20341 cttgtagaga tagcggatta tttaagtagt aagaatatac catttacgat tgcattaata
    20401 ccagcatatg taaatcctaa aaatcataag gtaataactt tatcagaaag ccctgaaatt
    20461 gtcaaagcaa taaaatatat gcaagataaa ggtggaactg tgatattgca tggatatact
    20521 catcaataca agaaagaaga agtttctgga gaagggtatg aattttggga tggtaaaaaa
    20581 gatgaaccct taaaagaaaa tatgaagata tttgtcaaag atagagtttt gaatggtctt
    20641 agagtatgta tagagaatgg aatttatcct cttgcatttg aagcgccaca ttatgcaatg
    20701 gaatcagagg gatataaaga attaaaaaaa tatttttcta cttatatagg gcaacatcaa
    20761 aataatgata aaaaatttag cacaaataca tatccttata ttattaggga tacagaggag
    20821 tttaatatat ttatacccga aaatttagga tatatagacc cagaagataa gtttactttt
    20881 caaaatataa aagaaaattt agataaatta tccattgtaa gaggttttag tggagggttc
    20941 ttctttcatt catatttaaa catagagtat ctaaaaaaca caatagaata tttagaaaaa
    21001 cagaatatag aatttatgaa tttaagagat tttaataatt gggtaaaagt tgatgaaata
    21061 caaataagaa ataatggaga tgaaattata gtcaactatg ataaggactt agatgaacta
    21121 actaaaagtg atactagatt taaatccata tccaatatta gtaaagtttt aatattcata
    21181 gtatctataa gtgttttaat atttgtgatt atatttatat attttaagag aatagataaa
    21241 aagaaatttt taaaataaag gattttatat tagaatcaat attttaaaat attttaggag
    21301 aaatttatga gagatttaaa tgtatcagag tatttgttcg tatttagttt gtttagtata
    21361 tggtctttat tgcttataaa tattatttta gctatgggtg gatatatatt ttattttaaa
    21421 aactttgata aagaaataaa agaaattgat gaatatccta tgatatcaat acttgttcct
    21481 gctcataatg aagctaaagt gataggtaga actgtggagt ctttgctatt actcaattat
    21541 cctaagagta agatggaact tattgttata aatgacaatt cttctgataa tagtaaagaa
    21601 attttagaaa atataaagga taggtacaac aattataatt tcactataat aaatacagat
    21661 agtttaacag gtggaaaagg aaagtctaat gcattaaata ttggatatac gatttcaaaa
    21721 ggagatttta tagctgttta tgatgcagat aacacaccag ataaaaatgc acttagatat
    21781 ctagtacaaa ctatagtaat gaatgatgaa ttaggtgctg ttattgggaa atttaggact
    21841 cgaaataaaa ataaaaacct attaaccaaa tttataaata tagaaacatt aagctttcaa
    21901 tggatgtcac aagctggtag atggcaactt tttaatctat gtacaatacc aggaactaac
    21961 tttatattga ggagaagtat catagaagaa attggaggat gggatagtaa agcaattgca
    22021 gaagatacag aaataagttt taggatatat aaattgggat acaagataaa gcttgtacca
    22081 caatctataa catgggaaca agagccagaa actgttaagg tgtggataaa gcaacgtact
    22141 agatgggcaa aagggaatat atatgtatta atgaaatata taaaaaatat atttaaacag
    22201 ggaagaaata agattgtgtt tgatatagct tacttttttt ctgtatattt tctattttta
    22261 acatctgtaa ttatatccga tattttattt gttttaagta tatctaaact agtagaaatt
    22321 tctattccaa ttaacttttt tctaatatgg atactatctt atcttttatt tattatagaa
    22381 gtgagtataa gccttactat agaaaaaggt gaagcaacaa ttgaaaatat attcatagtg
    22441 gcaataatgt attttacata ctctcaacta tggctgtttg tagctataaa aggaatgata
    22501 gagtatctaa aagatataat ttttaaaaga gaggttaaat ggtataaaac agaaagattt
    22561 tgattcatgt acaggaggaa ttaacatgaa aaaatttatt atatcaatta tatctttagt
    22621 attgtttttt tcaaatatat cacttatata caaagtaaat gctgatgaga ctaaagttaa
    22681 aaattataag tttgaaagag atataactat tgatggtgtt ataggaagta atagtacttt
    22741 ttttgaagta aataaaaatt gggatattga agaagtttta ttgcatctta attttagtaa
    22801 gagccaaata ttaaatggag atgtttcaag tttaactgta ttgataaata atgtaccaat
    22861 aaaatctatt aaattaaatg ctaagacaaa ttataaaaat acattagaag tattagttcc
    22921 aaaagattat ataattcaag gatataatga aataaaaata aaaacataca aaacaatatc
    22981 tgataaaatt tgccaagatg attccaatac tgggaactgg atggttattc acaaagaatc
    23041 atatatatca attagatata agcagaaaaa ggtggaaaac tcaataaatg aatatccgta
    23101 tccatatgca gaaattgaaa ataatcataa attagataca actatagtag taccagataa
    23161 tatgactaga ggagagacta cagcagtgtt taatttggca tcagcatttg gaaaaataac
    23221 aaaaaatgat gatttaaagt tagatgttaa attatattca gaaatgaaaa attggtcaga
    23281 tgataatatt atatatattg gtaaacctga aaatacagca gaagaaattt tggatatttt
    23341 aagtataaag gaacaaaccc tattatcttc aaactgtata atcaagcaag tagattcacc
    23401 atacaataag aataagaaga tgatggtggt aattggtagt aatgaagatg atttaataaa
    23461 ggcatcaaat ttattaatag aaaatagact ttctaaccaa gttttatcat catcagtact
    23521 tgtaaacaaa gagactaata taaaaattaa tagggaacaa aaattaaatt taggtcattt
    23581 aacattaaaa gatttaggat attctgactt tttgttggaa ggagcattta atcaacaagc
    23641 tttgtttgat gtcaaaatac caacgggtaa ggtattagat gatggttcaa agattatctt
    23701 aaatttaaga tattctgaca atttagattt tgaaaaatct ttagttacag tgtctataaa
    23761 tgatgttatt gtgggaagta aaaaattgga taggtcacat tctaataatg ataagttaga
    23821 attaaaaata cctaaagata tagataataa aaactattat caagttaaat tgacatttaa
    23881 tttgagtata aaaaattcga attgtgttac tagagagagt aataatcctt gggcatatgt
    23941 atcaaataat tcttatttgg cgttatctac gaaagaaaat gaaaccttat cttttgaaaa
    24001 ttatccgtat ccatttgtta gagatgatga atttaatgat ttaactgtga taatgccaga
    24061 ttattcaggg tcacaagcta tgacctggat gtttagatta ggtgttactt taggtgcgaa
    24121 tataaattca cataatggaa atataaatgt aattagagga aaagaattta gtgacaagta
    24181 taaggatact aatattgtag tttttggagt gccacataat aattctgtta taaagatgtt
    24241 aaataataat ttaaatataa agttcgataa aaattactcc aattttatat caaatgataa
    24301 gataagcttt attgatgatt atggaaaaaa tatttctact attcaattaa taaaatcacc
    24361 atataataat caaaaaaata tcatggtaat tagttcaatg aatgaaaaaa acttatattt
    24421 aggaatggat tatttgttga ataaaagtaa agttaatgat ttaaaaggag atacattgat
    24481 aattgatgaa tacggagagg tagaagattt agcttataat ttaaaatcta agaaagaagt
    24541 aaaagattca agttggaata tgagtataaa taagacaact aaagtatttt taatgatttc
    24601 ttttattact ataattatag tcatgatatt atcaatgcta tatataaaaa aatataaaag
    24661 aagataatat gataaattta tt
//
LOCUS       DAYAOX010000039        24301 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.039, whole genome shotgun sequence.
ACCESSION   DAYAOX010000039 DAYAOX010000000
VERSION     DAYAOX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24301
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.039"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(54..935)
                     /locus_tag="ACGI57_001248"
     CDS             complement(54..935)
                     /locus_tag="ACGI57_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9716333.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            1208..2596
                     /locus_tag="ACGI57_001249"
     CDS             1208..2596
                     /locus_tag="ACGI57_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HFG9716334.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYANGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            2624..3577
                     /locus_tag="ACGI57_001250"
     CDS             2624..3577
                     /locus_tag="ACGI57_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9716335.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            3637..3861
                     /locus_tag="ACGI57_001251"
     CDS             3637..3861
                     /locus_tag="ACGI57_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716336.1"
                     /translation="MNEKSSFKQIEIILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            3854..4366
                     /locus_tag="ACGI57_001252"
     CDS             3854..4366
                     /locus_tag="ACGI57_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFG9716337.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            4398..5252
                     /locus_tag="ACGI57_001253"
     CDS             4398..5252
                     /locus_tag="ACGI57_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HFG9716338.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            5786..6580
                     /locus_tag="ACGI57_001254"
     CDS             5786..6580
                     /locus_tag="ACGI57_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9716339.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            6684..7490
                     /locus_tag="ACGI57_001255"
     CDS             6684..7490
                     /locus_tag="ACGI57_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFG9716340.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            7552..8073
                     /locus_tag="ACGI57_001256"
     CDS             7552..8073
                     /locus_tag="ACGI57_001256"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFG9716341.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            8488..10575
                     /locus_tag="ACGI57_001257"
     CDS             8488..10575
                     /locus_tag="ACGI57_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9716342.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMDDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            10590..10877
                     /locus_tag="ACGI57_001258"
     CDS             10590..10877
                     /locus_tag="ACGI57_001258"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9716343.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            10904..12175
                     /locus_tag="ACGI57_001259"
     CDS             10904..12175
                     /locus_tag="ACGI57_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFG9716344.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            12233..12832
                     /locus_tag="ACGI57_001260"
     CDS             12233..12832
                     /locus_tag="ACGI57_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9716345.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQEYLKWHPAGSLGKSIREGI"
     gene            12837..13742
                     /gene="dapA"
                     /locus_tag="ACGI57_001261"
     CDS             12837..13742
                     /gene="dapA"
                     /locus_tag="ACGI57_001261"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFG9716346.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKSQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            13848..14480
                     /locus_tag="ACGI57_001262"
     CDS             13848..14480
                     /locus_tag="ACGI57_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HFG9716347.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNANKIREIIKNVRKI"
     gene            14957..17464
                     /locus_tag="ACGI57_001263"
     CDS             14957..17464
                     /locus_tag="ACGI57_001263"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9716348.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            17806..19968
                     /locus_tag="ACGI57_001264"
     CDS             17806..19968
                     /locus_tag="ACGI57_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFG9716349.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            19973..20590
                     /locus_tag="ACGI57_001265"
     CDS             19973..20590
                     /locus_tag="ACGI57_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716350.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLDLSEQADRVIKL"
     gene            20779..21123
                     /locus_tag="ACGI57_001266"
     CDS             20779..21123
                     /locus_tag="ACGI57_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFG9716351.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            21693..22559
                     /locus_tag="ACGI57_001267"
     CDS             21693..22559
                     /locus_tag="ACGI57_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFG9716352.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPINK
                     ESGKPCINLQPDFKCSVHNSLIKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESMMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            22672..23076
                     /locus_tag="ACGI57_001268"
     CDS             22672..23076
                     /locus_tag="ACGI57_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HFG9716353.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            23240..23689
                     /locus_tag="ACGI57_001269"
     CDS             23240..23689
                     /locus_tag="ACGI57_001269"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9716354.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(23845..24222)
                     /locus_tag="ACGI57_001270"
     CDS             complement(23845..24222)
                     /locus_tag="ACGI57_001270"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9716355.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
BASE COUNT         9383 a         2695 c         4182 g         8041 t
ORIGIN      
        1 ttaactatag aaaaaatggt taatgctatt tttatcgtta atattgatta attttatatt
       61 atagaaatga acttttctaa tgctggattt gtattttttt tattccatac aactacaact
      121 tcaattctat tttctacttc ttttataggt actattgcaa tatcacgttc tatcattgta
      181 tctgtaacac aaaatgaagg taaaatggat attcccatgt tagaagatac caatgattca
      241 atagtattaa gattatttga acgttgtacc acattaggta caaaccctgc ttcacgatat
      301 ctattctgta tcattgactt ttgtccatac tcatcccctg tttcctttac aaaaataaat
      361 ttatcatgtt gtaattcaat taaatcgata gaatcctcat tgtaaagtgg atgaccagga
      421 ggtaaaagag ctaccaaaga atatctttct acagttttat attcaaaatc ttttaatggt
      481 tgattttctg gattaataac aaaagcgagg tccatagaat tattttttac taagtttaaa
      541 agttcggtta tttcattagt acaaatatcc atagatatat tagggtatgt attagaaaat
      601 tcttttaaat attgttgaag tttgtttttt tcatatccta ttaaggttcc aatgctcaaa
      661 gttccaaaaa aaccatgtgc aaattgttgt gtttttgcaa tagcatcatt gatatttttt
      721 attatagtac gtgcctcaga taaaaaaact tttccagctg gtgttagttc aacatgacgt
      781 ttacttcttg taaatagagt tacatttatt tgttcttcta aagcttttat ttgctgtgta
      841 acagcagttt gagaaatata aaatttttct gctgttttgg taaaacttag attttctgct
      901 acagatataa aatattctaa ttgtttagta ttcaaataat cacccctatt taaacttgat
      961 tgtttgtaaa ccttatttat ctataattaa agcttattat agtataagaa aatttacttt
     1021 taaagaatat tagaaaatat ttgtataaaa atatatgttt taagtatatc tttacattag
     1081 attttataaa tttattaata agaataactt atatattcca atgaaaacga tattggattt
     1141 aagtaagttt gtctcataaa atgaaagtat atcaattgat aatactttaa ataagaaggg
     1201 agaaaaaatg gaagataaat tttatgcaaa aggcaacgga aataacggat atattaaaaa
     1261 tcttgaagtt tgttccttta ataacttaga tggaacttgt ggaatgtttc aaatggctct
     1321 gtacaaaaga gatgaaaaat actatttata tggatgctgt tttggaggaa ataaaaaaaa
     1381 tggagtaatg attagcgata ttacagaccc ttataatcca caatttataa aacattttca
     1441 aatgttagac cctaaagagt atcctacaac aacaactccc aaaattcaaa tagcagatga
     1501 tttaatgata gtagcaatga gttgtggaag tggaccagga gcacttgttg accaagctaa
     1561 attagcaaat attaagtgtg aagcaggaat tagaatatat agtttaaaag aagacccttt
     1621 aaatcctaag tttttaggat attgggattg tggcttaaag catgtaatgg gtgttcatag
     1681 attcatgtac aatggtggaa gatatgtaca tttatcaagt gattgtgttg gctttgaagg
     1741 tctgatttat agggtcatag atataataaa tcctactaat ccagtggaaa taggtaaatg
     1801 gtggagacca gaccaatatg caaatggata tccaaataga acttttgatg caggagcacc
     1861 tcattgccca gaatttatgg ataaaggatg gcttcatgga cctccatttg taagagacgg
     1921 aaaagcatat tgtggttatg gaggagctgg tttagttgta ttagatgttg aagatttaac
     1981 aagaccaaga tgcttaggtg aattgccatt tatgcctgca ttttctagta gacttgcagg
     2041 tgcaagaact catacagcat taccattgcc aggaagagat ttagtcgttg ttcaaaatga
     2101 gggagaaaga ttccagttct ttaaaccaga taacattaca gatgttcaag ctatgaataa
     2161 tatacatatg gttgatgtta gtgacccaac aaaaccaaca ttaattgctc aatttccata
     2221 tcctgaagtt ccaaaagatt tcccttatcc taactttaat gttgcgggat taggaaaacc
     2281 agggccattt ggcccacata atcttcatga accaatggat aataagccat ggttagagca
     2341 aagaggagat agagtatatt gctgttattt ccatgcaggg ctaagggttt atgatgtatc
     2401 agacccatat tatatcaaag agctagcata ttttatacca ccaaatccaa ataaaacacc
     2461 agaagaatct tatttcccag gattcccagg accacgcttg gcagtaacag aagatcttat
     2521 cgttgatgat agaggctaca tcatcataga tgctttagat gatggattct atatattaaa
     2581 aatgaaagaa gattaaataa atactaaaga gggagagttg aaaatgaaaa tactaggtat
     2641 ttcttttgga acaaaaaatg gaaataatga cacaatgtgt aaagttgcac ttaaaggtgc
     2701 caaagaagca ggggctgaag tagaatttat tcaaatgtca tctattaaca ttaaacattg
     2761 tactggatgt tgtgcgtgtg ttaaaacttt attatctggg aaaggtagta tgtgtgtatt
     2821 aaaagatgat tttgaatggt tgcttgacaa aatgaaagat gcagatggta tagtagtttc
     2881 agaccctatt tttgaagaag gggcatcagg attattccat actataatgg accgttttgg
     2941 acccagagca gatagaggaa ataacattat aggaacaaaa gttgcagaag ctataggagg
     3001 aaaaattcca gattcaagaa tgcttaatga taaagtaata tcatttatgg gaataggagg
     3061 ttctgactgg ggtacaagag ttcaatgtga acatgctatg ctagctctca taccaatgtg
     3121 gaaagttata gataatgctt ggttcccatg ggcaaaggaa ttagtaatgg atgatacaag
     3181 gcttgttcaa gttcatcaaa ttggtttaaa tatagttgaa gcagcaaaag attttgaaaa
     3241 agcttcatat caaggagaag aaggtgtatg tccacattgc cacaatagat tattctacct
     3301 tgagccaggt actaaaaagg caatttgtgc tctatgtggt atagttggag agttagatac
     3361 tataagtgga aaaactatat ttagttttcc agaagagcaa ttaggtcatg ctcatgatac
     3421 tttaccaggt aagtttatac atggtgatga tattaagaaa atggaaggtc attatgcaga
     3481 agttcgtaaa acacaagaat ttaaagaaag aaaaacatct tataatttta tagagccatt
     3541 aactaaagaa aagcaagctg gagtttcatt aggataataa ttaaaatact aagactcttt
     3601 tttaaggtat gttagatgtt ataggaggct tattgtatga atgaaaagtc aagttttaaa
     3661 caaattgaaa ttattttagc agttattttt ttggtactta catattacaa ggttagatta
     3721 actccagcat ttattgttat atttatacca ctatatttaa gttattttgc agtatctatg
     3781 attgtaaatc aaatagcaat taagctattg gagataaggc aagctaggga agctactaat
     3841 tacaatatag gaaatggata gaaagaaaaa agaaatatta acactgtcta tggggattgc
     3901 tttactacct cctttatggg cagtattagc accttatata ggaataaaaa ctggagctgt
     3961 agcactaatc tgtgcaggat tatatgtaac taatggtaat aaacaaaaag atggattaaa
     4021 aataatgttt ggtttttggt gtggagattt atgggcagtg ttagccattt taattatggg
     4081 atatatgaat ttcaatcaaa acctagaatt atttttaaca cttagtatat taggtttttt
     4141 tgcagttgta attgcatcct tatttgaaaa aataattttc ctaccatcat ggctttgtgg
     4201 ttgggctata ggtctaacta ttatgacagg tgaaaatatt attaatttac aagatatttg
     4261 tattcaaatt gctgtagcta tggcagtagg agtatggtac gtgggtgcag gtgtagattt
     4321 gtttacaata tatattttga gtaaagatag gaaaaaggga aattgaaagc atgtgaatat
     4381 actttgatta taggagaatg gctatgggtt ttgaatttaa aataatgaga agtttaatat
     4441 atgtaggact tgccaaggaa gaatatagac ctaagctaat ggactggtta tatcgtcacc
     4501 atattccaga tagtattagc acttttggac catattgtac taaatatgcc ttttatcaag
     4561 catatcctac accaaatgaa ggtgagcgtt ttggtgcacg taagatgcaa ctaacagaac
     4621 attattggct tgtagatgaa catatgcctg agatggcaaa tagaattatg acagaatata
     4681 tgcctatgga tgttctacgt tggcaagggt gtataccaga tgtagaaaat aaaagggttc
     4741 atgaaaatgc agaaagtgga gatgcaggac gtgcagtagg tggagataat ggatgtccac
     4801 catttatatt tgcctttgtt ccaataaact gggaagaaga ctttagagga aaaggacgta
     4861 ctgtacaaga tggaccaaac tatcgttggc aatttatgat taagtatcca gatggtatct
     4921 ctaaagaaga aggagaaaaa tggttctatg atgaggtagt gccatacttt acaaactgtt
     4981 gctatgttaa tcgttttgtc agtagtaaaa taatgattaa ttatggagca actgcttttg
     5041 accgtgtatc agaactatgg tttgaagggg aagaagaatg gtataaagct gtggttgaag
     5101 agacaaagtc gtttattaaa aaaccagaat gggcacaaga agaggagttc ccatatttaa
     5161 aaccacaatt caatatcgca tcagtattct taggtgatat agcaactatg gatgcatact
     5221 cacagtatcg tggatatata ccaatgagat aaaaattata gtttgtgata agtagaggag
     5281 gcatttactc ttactggtag aagaagactt attgtatcct taaaagtagg tgaatatata
     5341 gttttatatt taaatttata taaaactaag actaataaat atgaaacaat agaattagaa
     5401 taatttgata atatttgtta aaagagcatt tagaatgtgt ttaaagatta agatattcct
     5461 tttatggtag cagatagttg aaataataaa actgttatca tgaaaggaat atttttatgt
     5521 ataggaaatt tttaaaaata aaagttaaat atgtttaaga acatcttaaa gtgaaagaat
     5581 cattgttaga aatatgtaga caatatagtc tttataacgt atcatattaa caagtttaca
     5641 cttggattat gaaataaaaa gagtaattcc aaaggttgta aatactatgc tgactttgat
     5701 acttcaaagt ttaggtgatt ttgtaattgt taatagaaat acgtttaatg agcataaaga
     5761 atttatctaa taataggaga atataatgta tacaattgga caagtatcta aatttttagg
     5821 aatatctaga gatacactaa aattttatga ggacaaagga ttagtaaatc ctaagaaaaa
     5881 tgatgaaaat ggatatagaa tatataatca ggttgatatc tatgatattg caacaataaa
     5941 cttttataga gaaattgata tagaaattaa aaagattcaa gaaataagaa aaagtaagag
     6001 tataaacaat ttagaactct tactagaaga gaaggaacaa atcatactaa aagagataga
     6061 atataaaaag cttctcttaa aaaaggttca gttagtcaaa gaagattgtg acaaaataaa
     6121 aacatatttt ggaaaataca caataaaaga aatgaagcct ttggaagtaa aaggtaaaat
     6181 aaaacatgtt actaactatg aggaatatga agccttaaaa gagaatacaa acaacttaaa
     6241 aaaagcagtt acattaactg atttaagaag agtaataaat tttaatgaag aaggtataat
     6301 agaagaaaaa tttataattg taagggaagt agatgactac aacaaaatca tagaaggtga
     6361 agttctatct catcccaatt gtatttatac tattattgaa gatggcagat ggtcaactgg
     6421 aggagaaaat attgataata aagtggaaga cagattaaga gaaatagcaa tagaaaatga
     6481 atgtgaatta cttggaatag tctatgttaa tatattgctt actacttatg aagaagggat
     6541 tgagcgtata tttttagaga tttatgcacc tataaaataa tataaaagca ttgacctggg
     6601 acttactcat aggtttaaac tcatgatata agcagttata acttatatag caattaaata
     6661 ttaatttgaa atgaggatta cttatgagaa cagagaaatc attaaaaagg cctatagcca
     6721 gttttattat tctaacaaat ataatttttt taccactttt tttattggta gtagtgacta
     6781 taatgttaaa acttccaaca gtgatatctg acattgcgtt gtgtatatca gcgtggtcat
     6841 ccacttttgc atttatgata ttatttaaaa aaatttatcc aggtaagaaa tttttagtac
     6901 acgtaaaaga tagatttaga aataaactta aattttcaac agtatctata ataattagta
     6961 tacaagtcct aatagctgta gtagttatat ttatattagc atctaaaaat catggaataa
     7021 tgcctaactt tacagtttca tcatggggag catttatata tttattttta aaaaatttat
     7081 ttgcaggatc attaggtgag gaattaggtt ggagaggatt tgttcaaaat catcttcaga
     7141 aaagacattc tcctttaaaa gcatccataa tagtaggttt ttggtggggg atgtggcatt
     7201 taccaatatg gtttacaaca ggattcaaag gacttgattt aataaaatat attatatttt
     7261 ttatgattgc aattatatca atcagtatta taatgacaac tttttataat ctaaataaaa
     7321 ctttgataat acctattgta atacatcaac tatttaattt tttaataggg ataattaatg
     7381 gagatttaat agagcttata aattactatg caatgttata tttaatattt gcaattgcat
     7441 taatagttat aaatccagga aatgtattat ataaaaatat tagagagtaa tatatatctt
     7501 tatggtaaga tataaagata ttattagaag aacatccaag gaggaataaa catggtttta
     7561 ctgattgttg atgcacaaaa gttaataaca aacgagaggt tatataaatt taatgaattt
     7621 gtagtaaatg tagaaaattt aattgataca gctagaaaaa ataatataga agtaatatat
     7681 gtacgtcatg atgatggagt tgaaaatgaa ttgactaaag gtaaaaatgg ctttgaaata
     7741 tacgaaaaat ttaaaccatg taatggagaa aaaatatttg ataaaaaagt aaatagtgct
     7801 tttaaagaaa ctggtctatt agaataccta acaaataatg gtgaaaaaga tattattatt
     7861 gctggtctcc aaacagatta ttgtattgat gccactataa aatgtggatt tgaacatgga
     7921 tttaatataa tagttccagc ttattcaaat acaactgttg acaatttttt tatgtcagca
     7981 gaacaatcat ataaatacta caatgaattt atatggaatg aaagatatgc gaagtgtatt
     8041 tcattagagg aaacacttag aaaaatgaaa taaaaataga gtatgaattg tatatgtaca
     8101 taagttgatt tcataaaaaa ggatgttaaa ttccaagtta aaaattagat taaatatatt
     8161 atattataga taaggcacat caattttatt ttaaattaat gtgcttttta attgtagtaa
     8221 tttatttata atcaatatga tttttattat ttaaatcaat ttttcttgag tttctttgtc
     8281 taaagaaact ttttttattt ataaaatcta cagttatatt atagtatcta ttttagagaa
     8341 gtttaatttt tgtatcaatc taaaacaaaa gttatactta aatgaaattg aaaacgataa
     8401 aattctcaaa taaaataagt attgtggagg tgatgaaaga atccaatgat aatatgaaga
     8461 ctaatcttaa taagaaagta ggggttaatg gtagtggaca aagaaattgt atcaattctc
     8521 cagattttat taaatagcac attgattaca actaatggat tacaagagga gtctaattca
     8581 tcaaagagac aaattactta tagaataaat aaaatcaatg acatgcttaa agtaaaaaat
     8641 gtaccactta tatgtttaag agctgataaa gatattatcg ttagaaaaga aacaaaaaaa
     8701 gcaattaaag aaattttaga aaaaaactat tctaagaata cttattattt tagtaagaca
     8761 gaaagacttt tatatatgta tttaatgctt tttattaaca tggatgatat ttcaattaat
     8821 cattttataa attctataaa agtaagtagg tctactgtaa atcttgattt caaggatttg
     8881 ataccagagc ttgaaaagaa gaacattaaa gttaagaaca atcgtataaa tggatattac
     8941 atagttggca atgagatgga aattaggaga gtgcttatta aaaatattat agaaactttg
     9001 tctaatgaga aaagttcaag agtatttgat atttttatta aagaatataa tttagatagt
     9061 tttgaagagg ctaaaaaaat tatattaaag ctaataaaaa aatatgaaat tacttttgtt
     9121 gaagatagat tagtagaatt tatatatata tttatctttc taaaggcaag gatgtattca
     9181 actagaatag ttactcagga aaatgtagat attcctaata tttcagttat gagttcaatg
     9241 aaggagtata aatttgctaa ggaattatta gaagtatatg ggagtgaaga taagataaag
     9301 cctcacaata taatgtatat tagtgcttgg attttaggta tatcagtagg aaattatgaa
     9361 gaagatacag aagataagga tgtaatttca aaaattgtaa ataaaatgat gacaaagttt
     9421 ggatatttaa gcggagttta ctatataagc caagaaaaaa tatttaaaca attatattct
     9481 cattttagac ctgcctacta tagactttta tttaaattac ctatttataa tccgatttgt
     9541 gaaaaagtaa aagaagaata taaattagtt tatagaattg taagtaatgc aatgaaagag
     9601 ttttgcggat tatttggaga agaaattcag gaagaagagt tagcatatct tacaatgcac
     9661 tttgcaacac ttttttcaaa taaaaaagaa tttgatggtt ttagaaaaag aatagcatta
     9721 atagtttgtt caaatggcgt tggtagttca gctattttac atgcagaact tacaaactta
     9781 tttcctgact tacactttat accaatgtcg gaatcagttg gattagaaaa tataagtgaa
     9841 cctgtagata ttgtatttac tactaattat aacgcagagg gtttaaaaac agatgtacca
     9901 gtaattaaag taagccctgt tatgaccaca aaagaaaaat ataagattac acgtgaagtg
     9961 tatattcaat taggtgatat ttttttaaga caacctaaaa tagatgaggt tatggatata
    10021 ataaaaaaat atgcaaatgt aacctctgaa agtattcttt caagtgagct attagcttat
    10081 tttacacaga tagagaattt tacatctaag gaaggagagg ggcctatgct aagtgagatt
    10141 actaatgaaa cactagtaaa aataaaggta aaagctaaga attgggagga agctattaga
    10201 cagtcagctt ctgtattagt tgaaaataac aaagtaactg aagggtatgt agatgctatg
    10261 attaactcag caaaagcatc tggaccatac atagtaatta caaaacatgt tgcacttcca
    10321 catgctagac cagaagctgg gtcaaaagag attgcaatag gcattgctac tttagatagt
    10381 cctattgagt ttgggaataa cgaaaacgac cctgtaaaat atgtattttg tctaagtgca
    10441 atagacaata atagccattt acgtgctatg tctgagctag ttgaactatt ggaagaagat
    10501 gagtttttta aggttttaga tagtgctaaa gaagctaaag aaattattga ttttataaaa
    10561 acacacgaat tataaaggag gatttaatta tgtataaaat tttaatcgca tgtagagcag
    10621 gagttggctc aagtttaatg ttaaaaataa agactcaaca aattattaag gaaaataact
    10681 ttccaattga agtagaacat ggttcattgg attcactaaa tggattcaat ggagatgctg
    10741 tagaaacatt aattgatgtt gctgaagaat taaaaaataa aaatttgaag tttgatataa
    10801 ttgggattca caatattgtt gaccgtaatg aaataaagac agcactagaa aaattccttg
    10861 cttcaaaaga gggataaatt taagatgata aggaggaata tttatggagt ttattattag
    10921 tttgctgagt acacctgctg ttttgcttgg cgtagttgca tgtataggat tgattcttca
    10981 aaagaaatca gctgttgaag tatttacagg ctcttcaaaa acacttatcg gttttttgat
    11041 atttggtatt ggtgcctctg ctatgacagg agcacttcaa aatttcaata aactgttcca
    11101 acatggcttt ggaattactg gtgttatagc ttcaccagaa gcagctacag cacttgcaca
    11161 aggtacatat ggttttgcag tttcatgtac attaattctt ggattcatat taaatttagt
    11221 atttgcacgt attacaaagt ttaagaatat tttctttaca acaggtcata gtttgttctt
    11281 ttcttgtgta ttagttttaa ttatgaaagc acatggattt gataatacaa tcactatttt
    11341 aataggtgga actatcttag ggtttatgtc tgctgcacta cctcaattct gtcaaccatt
    11401 tatgcgtgaa ttaacaggtg gcgacgaaca agctattggt cactttaaca tgattggtta
    11461 tggtttatca ggttatattg ggcgtttatt ttcaaaacat aaagatgata caacagagac
    11521 tatagaattt cctaagtggt tatcaatatt cagagacttc ttaatgggtc tatccatagt
    11581 tatgttaatc ttattttata ttgcaacatt aaaagctggt caagcattta ctcaagaaat
    11641 tgctggttca acacattggt tggtttttcc gcttatagag gcatttacat ttgtggcagg
    11701 tatgtcaata ttgatgtcag gagttcgtat gttcttagct gaaataactg cagcatttgt
    11761 aatcatttca gaaaagtata ttccaggttc acgtcctgcg ctagatgttc caaccgtatt
    11821 tccatatgca ccaaatgcag ttattattgg gtttatatca gcatatgctg ctggtctatt
    11881 agcaattgct attatggcat catttcctag tatatttcct gtagtaatta ttccagctgc
    11941 tcacatatgt ttcttctcag gaggtacagc tgcaatattt ggcaatactt caggaggatg
    12001 gagaggggct attgctgggt catttgttgt ggggttatta cttgcatttt taccagttat
    12061 actatatcca gtttatggga ctcttggtat agaaggagca actttcccta atattgatta
    12121 caacgtagtt ggttcgatat tacataatat tttacaattt ataaaaccta tttagactaa
    12181 attatataaa agtatagtca tagttactat gatttcaagg aggatacatc ttatggaaaa
    12241 tacattatct gaatttttag ataatatatc taatgaattg tcagaattta ttgcctcaat
    12301 agatgagaca tctataaata aagcatgcga attaattctt gaagctgaaa aaaatcacgg
    12361 acgtgtacat gtgactggta ttggcaaacc aggacatgta tcgggatata tttcatcatt
    12421 gctttcatct actggaacgt cagcatatat attgcatggt acagaagcag tacatgggtc
    12481 aagtggtcaa gtagttgaag gagatgttgt tattgcaata tcaaatagtg gtgaaactca
    12541 agagttaaaa gcaactctta agactctaaa agtaaatgga gcaaagataa ttggtgtaag
    12601 tggtaatgag agttcatttt taaaaaatat ttctgatata ttcctgtttg caggtgttaa
    12661 gcaggaagga gattgtctca ataaagctcc tcgtgcttca attttagctg aaacaataat
    12721 attacaatca ttaagtgttg tattacaata tgctaaaggt cttaatacac aggagtattt
    12781 gaagtggcat ccagcaggaa gtcttggaaa gtctattaga gagggaatat aattttatga
    12841 aatataaagg gattatatgt gcaatgataa caccatttga tgaaaatcaa aatattaatt
    12901 cacaagcaac atgtcaatta attgattatt tgattgaaaa gggaatttat ggattattta
    12961 ttttgggcac taatggtgaa tgtcatgtat taacagatga tgaaaaagtt gaatttgcaa
    13021 agattgttat taatcataca aataatcgtg tacctgtttt tgttggaaca gggggtaatt
    13081 caactcgtga agttgtagat ttatctaaga gaatggaagc gattggagca agtgcattat
    13141 ctattattac accatacttt gttacaccaa cacaacagga attaatctta cattacaaaa
    13201 ctattgctaa ttcagttaat ttacctatta taatgtataa tatgccaggt aagactggaa
    13261 ttaatattga accagagtca gtacgtgaat tatcaaagat aaagaatatt gttggaatta
    13321 aggatagtag tggtaaactt gacaacatga aagcttatat tgaagcaacg aagaatgaga
    13381 attttagtgt gttttcaggc tcagattcac ttattctgga tagtctaaaa gctggtagcc
    13441 aaggagctgt tgcagcaaca acaaacttcc tcacagagat tggtatctct atctatgaaa
    13501 actttatgaa aggggatttg gaagaagctc aaaaatctca aagtagtata gaagaacttc
    13561 gacgtatact gaaacttgga acaattccat ctgttatgaa gaaaactatt gtcttgaatg
    13621 gaattaatgt tggaactgca cgacttcctg taactgaacc aactggggaa gtacttgaag
    13681 aaattaaacg agtagttgaa aattatagga caatacttaa taaaagtagt gagaatagat
    13741 aatataaata gggaaattac actggtatat aattaatata tatattataa aacatgacta
    13801 aaattaatga ataagtaaag tggtttgatg agtatagagg tgataatatg aaaaagatga
    13861 atataaccaa aagaatgtca gaatgtgggg ctcttgcaat tgtaagagaa gaaaatctta
    13921 accgtgcatg tgaaattgct gaaggatgta taaaaggtgg tataacagtt atagaaatga
    13981 gttataccct aaataatgct ggagaaataa ttcaagggct taataaaaag tatggtgaaa
    14041 tactttgtgt gggagcagga acagttttgg atagtgaaac agctcgtcat gctatattac
    14101 atggagccca atttattatt gcaccaaatt ataatgaagg tgtagcgaag atttgcaata
    14161 gatatcagat tccttatgct ccaggatgta catcattgac tgaggcagta gacgcactaa
    14221 gtttaggagc aacttttata aaagctttcc caatatcaga tttctatgga cctaaactag
    14281 taaaggtatt taagacacca atacctgata tgccagtttt agcaagtgga gggcttaata
    14341 ttgaaaacct acatatatgg ttagaaaatg gagttgatgt atgtggattt ggtggtttat
    14401 taactaaagg ttctgttgaa gatattgcta aaaatgctaa caagattcgt gaaatcatta
    14461 aaaatgtaag aaaaatttaa catagggctg tatcatagta tcttataatt actatgatac
    14521 agccttttta atattattca aatttaaaac agtttacatt tctattatat tacatataat
    14581 ttcagtaatt ttatcttaat ttttctatct ggaaaaaaat gatttaaaac ttaagttatg
    14641 atataatttc attaaaagaa ggcgattttt agtaaatatg agaaaataat gtgtttttag
    14701 gggaaaagtt aaatacagat ttaagtgaac aagaatcgat aattaagata ttaaatggtg
    14761 aatagggcat caaaaaaatt actaaattaa aagttttaga gggagaatct tagcaagtgt
    14821 gtgtgaaaat tcaatttcaa acaaaaataa ttgtaaatga aatcaattta tacacgttga
    14881 agattcactt ttatcattat attaaaaagc agagtgaatt aaaagtgagt ctaaagatag
    14941 gaggataatt aaaacaatga aagaagacat taggttacat cgaatgtgta aaaaagaaca
    15001 agcaacaaag aatgaagtta tatcagagga tattctaaaa tctatgagta ttggggtcag
    15061 taaacatttt ttagatgatt catttacaat aatatgggga aatacatcat tctatcaatt
    15121 attaggatat acagaagaag aatttttagt taaattttct agtttaaaag attattatga
    15181 acagaatctt tatcattttg aatctatgaa aaatcatttt ttagaggctt atcatcatgg
    15241 cgaaaagagt atagaatatc aaatttgcat ttcagcaaat aatggagacc ctgtttgggt
    15301 aataatgact ggtatattaa ttgaatcaca agataatgag aaatcaatgg tctatatatt
    15361 gtattcaaaa attaatgagt taaaaataaa gcaagaagaa ctatctgata tggaaaaaga
    15421 gagtacagag aattttaaat ggatgatgtc agagtatgct gggaatgttt atattagcga
    15481 tatggataca tatgaattac tatacttaaa taagcactca tgcaatactt tacaggcatc
    15541 agcaaatgaa cttattggaa gaaaatgtta tgaggtaatt caaggtagaa cttcgccatg
    15601 tccgttttgt acaaattcgt atttaaaaaa agatgacata tatgaatggg aattttataa
    15661 taaaaaactg aaacgaacat ttataattaa agaccgtatg ttgaattggc aagggcgaag
    15721 agttcgtatt gaattatctt atgatatgta tagtgctgaa tataaattag caaaaaaaga
    15781 tcaggaaaga gaatcaattt tgaaaactat tccagcagga atggttagat tagatgcaag
    15841 agattatctt actatacttt ggtgtaatga tatatttttg aatatgattg aatatacaaa
    15901 ggaacagttt gcagaggaac ttaataatca atgtggatat ttattttctg aagattataa
    15961 acgtagtaag aaactggcac aagatttgaa ggagtctggg gataatgttg tatttgaagc
    16021 aaaaatatat acacgttcaa gggaagaacg tatatggact gttacattgt gttatatcag
    16081 tggggaagac agttgggatg gaataccatc tttttatagt ataggtattg acataacaaa
    16141 ggaaagaaaa cagattgaga aattacagca tatatcagag aaagatgcat taacaggaat
    16201 ttataatcgt gctgagactg aaaggcagat taagaaatat tttgaaaaaa atttaaatgt
    16261 tatgggtgca ctgtttatga ttgatacaga taattttaaa cagattaatg atacagaagg
    16321 gcatatgatt ggagatatag tacttactga gatggcatca ggtatgaaaa aaataatgcg
    16381 tgacagtgat gttgttggaa gaataggtgg agatgagttt acaattttta tgaagaatat
    16441 ttcctcagtt aaagatgcag aaaagaaagc agaagaacta ttgtatatgt tccgtcatct
    16501 atttcagaaa gaaaaaagtt ttctaaaagt aacttgtagc ataggaatag ctatatatcc
    16561 aaaggatggt actacattca aggaaatata tgcaagagct gataaagccc tgtatcaagc
    16621 aaaaaatatg ggaaaaaata attatgtaat ctacaaccaa gattatttta aagaattaga
    16681 agaatttgat tgttcttctc ttggtacagt tattgattca gaaaaacaat acgctgaata
    16741 tcctgataat cttacacgtt atatattccg tatgctttat cagactgatg atatagacca
    16801 ggcgattaat atgatacttg aggttgttgg gaaacagttt gatgtaagtc gtgtttatat
    16861 ttttgaaaat acagaagatg gtagatatac ttctaatact tatgaatggt gtaatgaagg
    16921 aatctcttct caaatggctt atttgcaaaa cagcaattat caagactatg aggattatga
    16981 aaagcttttt gaggatgaat tagtttttta ttgtcgagat atccatacat taagtcctaa
    17041 actagaagaa ttgttttcta gtcaaggtat ccactctaca cttcaatgtg catataaaga
    17101 agatagagtt ttcagtggat ttgttggatt tgatgaatgc acaggactac gtctatggtc
    17161 acaagaggaa ataagtacat tatcattgat ttctcaaatt atatcaatgt ttttacaacg
    17221 taaaaaaaat aataaactta atcaacagat tcaacaatac cagaatattt tagataatat
    17281 tgaccagtgt atttgtgtga ttaataaaga aaataatttt ttattatatg taaataaaaa
    17341 atttaaaaat acatattcta aattccaaat tggacaacct tattatcttg attctataga
    17401 tattcataag atatccatta tatggaataa aaaagaagca tacctatgta ctagcacgat
    17461 ttaatggtaa agtaaagttt atattggact taaattaaat ttcgttttaa ataagatatt
    17521 attttagttg cttactgaaa agactagaat ctatattaat gaattatact aattttatac
    17581 cactattaaa atattgtttg ctaaaaataa aaaatagcct taaaacagtt gattataaaa
    17641 aattaactgc tttaaggcta tttttatctt tgtgacgaaa tagacacttg ccatttagaa
    17701 tgtgacaata tagtagttgt ttagaaaagg cacaaagcat tatactttaa ctaaagtaaa
    17761 atgtattttg cttttagaat aaaaacaatt aggagggagc tataattgaa aaagataata
    17821 tatgtattat ttacaattga actattaata gtatctttat taggtctaaa tatagttaaa
    17881 gataatgaaa ttaataatat tttatataac gatacaacaa gtatatctgt aatgtttaaa
    17941 gattataaaa agttaaataa aaattatagt aagtggatta aagatattgc agatgaaaat
    18001 aatgtggcta tatcaaagta tgtttttaat aataatgaaa aattatctat atatacgact
    18061 gatacaagtt tgaataacaa tataaagtta aaatcaggaa aatttcctaa tactaattca
    18121 aatgaattta ttagtaatat aaacactaat aatagtaatc aagtaggtaa attctcaact
    18181 atgagtaaag acatatcaat tttaattagg gattttggta aaataagtga agttggagaa
    18241 tcaggaatct tatatatttc aactcaaaat gaagatacaa taaataaaat attaagagaa
    18301 ctaaatgtag acagaagtat ttttgtagca agattacatg atagatatgt taactcaaat
    18361 ttatacttaa atccttcaat aattagagat ttaatactaa ttatcctttg tttcttggct
    18421 acaataatac attattcaat tagtgtgagt agagaggctt ctattttaag attaaatgga
    18481 tactctaagc tagatataat tgctagagtt ataaaaaata tacttagaat tatgatttta
    18541 tcatctgtca cagcattatt aatatatata atctatattt taaaattaaa tataggtgtt
    18601 agagcatgct tatactttgc tatgttttcg attatatgta ttttgataaa tattttgata
    18661 tctatattaa tagttggttt taatagtaga agttctaaat atgtgctatc tctaaatgga
    18721 aaaaaatctt atggttttgt aaatataatg catttcacat tgaaaattgt atttgtatca
    18781 tttcttatat tatccataaa taactgtatt ttaaattata atatgctaca aactcagtta
    18841 ggaaatctat ctgagtggga taaagcaaaa aatgtttata atcttactct taaagataca
    18901 ggcgaacaat ctttaggaaa ggaagaagta attaaaaatg ccaagattaa aggtttctat
    18961 aaaaatttgg tagaagaaaa agatgctttc ttaatagatt caactaattt tgaaaaactt
    19021 gaagatggaa gttacatgta tgaaataaat tcaaagggta aaaacccaga agtaagtcaa
    19081 tatggtaaaa atataaaaat aaataaaaac tatcttaaag ttaaccctat ttactcaaat
    19141 ggcaaagaaa tatttaatct aatcgattat gacgataaga ctataaattt attagttcct
    19201 aaaaagttac aagtacatga aaatgagatt aaaaaagagt ttagagaatt attttatttt
    19261 gaaaaaatag aagtagaaaa tatgtacaat aaagagttaa atagagagtt aaataaaaca
    19321 aagaagtccg atttaaatgt taatataata tatgtagata ataatcaaag ctattttact
    19381 tataactccc ttgttatgga tgacaatagg aatctcattg atgacccaat agcaatagtt
    19441 gacatagata atattgatga ttctttttat ctttcttata tatctagatg tgtatatttt
    19501 aattctaaga aattagatgc ccttgcagat ataagtagca ttattgaagc tcaaggtgta
    19561 gaagcgcata tccaaagttt acatgctgtt tataatgagt atggtttaga aataaataaa
    19621 ctagaaaaat atttgaatag tgaaattttt actataataa taatagcaat atcaaactta
    19681 atgattacct acaatatagt agctagctac tatgaaagaa ataaatataa gttatatatt
    19741 aaaaaaatat ttggttactc aactacagct agaagtatgt tgttaatagt ctcattaata
    19801 ttaacaaaca ttattccaat aggcattata tcaacaagag tagatttaag taataatatt
    19861 atcttatttg gacttctaat attagttata gaagtaattg tatctattgt attagacaag
    19921 attatctcaa atagttcatt taataaaatt ataaaaggag aacattaaaa agatgatttc
    19981 attaataaat atatctaaaa aatttaaaga taaatctata atagacaaat ttaacctaga
    20041 aatttctaag ggcgagtttg tagcgataac aggaaatagt ggttcaggta agacaacttt
    20101 acttaatata atgggattat tagaaaaacc taattctggt gatattataa taaataatat
    20161 aaaaaatcca aatagtaaac agataagaat tttacaaagg gatttttatg gctatctatt
    20221 tcaaaattat gctttaattg agaatgaaaa tgttgaaaat aacttgaaaa ttgctttaaa
    20281 gtatcaaaaa aatatcaatc ataatgaaaa aataaattac gcacttgagt ctgtaggtct
    20341 tagaaaatac aataaaaaaa agatatacga attgagtgga ggagaacaac aaagggtagc
    20401 attagcaaga gtaatattaa aagaatgtga agtaatattt gcagatgaac ctacaggtaa
    20461 tttagataaa aaaaatagag acatagtatt tgatatactt aaaagtctaa ataagaatgg
    20521 taaaactatc gtatttgtaa ctcatgattt agatttatcg gagcaggcag atagagttat
    20581 aaaattgtaa taatataaac tataataaat ataagaaaga gattacagct aaattaagtt
    20641 taataataat acgaatagaa tttaaacatt aagtaaatta gtagtttgta aatataagta
    20701 taatatgata tcataaaggc tcaaaagaaa ttttatataa taagaaatct aaaaataaca
    20761 aatggaggac atataaagat ggaaaattta ccaaattgcc cgaaatgtaa ttcagaatat
    20821 acttatgaag atggaactct tttagtttgt ccagaatgct ctcatgagtg ggcattagga
    20881 ttgggagctg aagaagattc aaatattatc aaagatgtaa atggaaatgt attaaatgat
    20941 ggagattctg ttacagtaat tagagacctt aaagtaaaag gaagttcatc atctataaag
    21001 attggaacaa aagtgaaaaa tatccgttta atccatgact catcagatgg acatgatata
    21061 gaatgtaaga tagatggttt tggagctatg aagctaaagt cttctgttgt taaaaaagcg
    21121 taatattata aataagctgt agtgtttatt gtttttcagt acagtataaa accataagaa
    21181 tctattaagt aataaaagtt tgtttattta tctttatatt ttcttatggt tttattaaat
    21241 ataaatttta ttgaacacca aaatattctc tcctctacaa ataagccaat cttatttaaa
    21301 ctaatatact ttgtactatt tatactgaaa ttgtaaattt aaatgctatg ttatttaggt
    21361 ttgtatagtt aatttatata tttctgattt tataaattgt atttattatt atatcttgat
    21421 tctaaaatca ttaaaaattt caacttctat aaagatttac aatttatcaa atacattatg
    21481 agtatataaa caatttatta ttacagtgct acatttaaaa taagtcttag tataaaggta
    21541 ttgttacaag tataaagaaa cttatgtatg ctattggaat aaataatacc ttaaattata
    21601 attaagaata agaactgtta aataagggac ttgattatca tttagttata gataatataa
    21661 tattaaaaat aaagtttaag aggagaaaaa atatgataga tatacataaa aatatatatg
    21721 ataataagtt atttgaggag ctaaagattg actgtaagaa gtgctttgga ctttgctgtg
    21781 tagctcttta tttttctgct tcagatggat ttcctatcaa taaggaatca ggaaaaccct
    21841 gtattaattt acaaccagat tttaaatgtt ctgtacataa tagtcttatt aagcgaggtt
    21901 ttaaagggtg tactgcatat gattgttttg gttcaggtca aaaagtagca caagtaacat
    21961 ataaggggat agactggatg caatcaccag aactaacaaa tcaaatgtct gaagtgtttt
    22021 taattatgag acaattacat gaaatgttat ggtatcttaa agaagcatct gtattaaata
    22081 tatcagatac aatcaaatca aaaattgatt taataattga agaaactgaa aagataacta
    22141 atatgggccc agaacagata attaatttgg atatcatatc acatagaaca aaggttaatt
    22201 tattattatc acaagcgagt gagtctatga tgggaaaggt taaatcattt atcaagactt
    22261 ctactcttaa aaacatgaaa aaattaagta aaaatataga tttaatagga gcagacctta
    22321 gaaaaataaa tctaatagga gcagatttaa gaggtagatt tctaatagca gcaaatttaa
    22381 gaaatacaga tttgagtggg gcaaatttaa taggagcaga cttaagagat tgtgatatta
    22441 gaggtgctaa tcttgaaaat agtatatttt taacacaatt acaagtaaat actgcaaaag
    22501 gagattctag tacgaagtta ccagcttcac taatacgccc taaatactgg gaaaaataat
    22561 taaattaata cataatttta caaaatatga acttttttga ctttaattca tattatacat
    22621 tagagattct ttacaagaat ttcttaatat aatataggga gagatataaa aatgagttat
    22681 caaaattata aatgtgaaag agattatgtg tatgatgatt ataaatacaa taagtgcaac
    22741 aagtgctata atgattatga ggaaatgaca catgttcatg aatattcaga aagtgttaag
    22801 ttagcagaag aatgtgagga tagacataac caccgtgcag caggggttac tggtgaagtt
    22861 ataccaataa atggaggtac aaatcatgtt cataaaataa atgataatgt agatttcctt
    22921 gaccatttcc ataagatatg tgttacaaca gggccagcaa ttagaatacc tggaactgat
    22981 aaacacattc acttaatatg tggagaaact actgttaatg atggtcattg tcataaattc
    23041 ctttttacta ctcaaataga agcaccttta gtataagctg atgtgaatta ttgtagtgaa
    23101 tttgctagat tatagtataa tatataatag ataatattta aacttaatga ggactatgat
    23161 atttaacctt cgtagtcctt tctaagtttt gtataaaaaa gtgttatgca attaaaagtg
    23221 atatagggag gaaattttta tgaaatctgg aataattctt gaaatgaaac aaataaattc
    23281 aacagaatat gacgaaatgt tgcaagtctg ggaatcatca gtaagagcaa cgcatgattt
    23341 tttaacagaa aaagatattg aatctttgat accattagtt gagattggac ttaaagaagt
    23401 agaaaatata gtttgtataa aagataatga tataataaag ggctttatag gaatagataa
    23461 agataaaatt gagatgctct ttatagaaga taaatataga ggaaatggta ttggtaaaaa
    23521 attaataaaa tatgctatag ataaatacaa tgtaaaatat gttgatgtaa atgagcagaa
    23581 taaaaaagct gtaggatttt atattcactt aggatttaaa gtgtttgata ggtctgaaat
    23641 agatggacaa gggaacccat ttccaatact acatatgaaa ttaatttaaa gttatacttc
    23701 ccacttattc gcaaaaatat ttacttaaat ttttatacaa ataaaagtcg tgacagtctt
    23761 aggacacgac ttttttacta cactattaaa ttgaaattta tctgttctat attatgatta
    23821 ttagatgtag tttttttatt ttaactatgt acttaaatac caagtatacc catacctatc
    23881 aagtacttca gcagcgccat cattttgatg tggtggtggg tgtggtaaaa attcttgtac
    23941 aagtgtacca ccttcggcaa gttttacaga tatttcttct atcgcttctc gagatggtag
    24001 aacaacacaa atcgccatgc cagaaattac aacaggcttg tctgtatccg cacccattat
    24061 acaaccttcg tattttgtga aagtaacact tgcgtgcata ataggattgt ttggattgct
    24121 gtctaatcgc gcaaatatag tttttcctcc tccgaaagcc tcagcataaa aattaaatgc
    24181 tttttcacaa gtaccattaa atattaaata tggagttagc atgaccaata tatcctcctt
    24241 gatatagaat aatttatagt tgcacctgat aatctaaatt atgatttagt aaattgtatc
    24301 a
//
LOCUS       DAYAOX010000040        24237 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.040, whole genome shotgun sequence.
ACCESSION   DAYAOX010000040 DAYAOX010000000
VERSION     DAYAOX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.040"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(140..910)
                     /locus_tag="ACGI57_001271"
     CDS             complement(140..910)
                     /locus_tag="ACGI57_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HFG9716356.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(912..2360)
                     /locus_tag="ACGI57_001272"
     CDS             complement(912..2360)
                     /locus_tag="ACGI57_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9716357.1"
                     /translation="MKRFLNKMMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVP
                     AISNISNIVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNA
                     GVFGGIIVGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIY
                     PQAAMNQFGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVI
                     RGEIPRFMAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALT
                     SFMATVTEPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSF
                     SYGDKAWLVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLS
                     HGNFSYMAKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLS
                     NTSVQIIIGTDVVKVKDEMEMQLDELRKQADA"
     gene            2730..3452
                     /locus_tag="ACGI57_001273"
     CDS             2730..3452
                     /locus_tag="ACGI57_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9716358.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            3649..5316
                     /locus_tag="ACGI57_001274"
     CDS             3649..5316
                     /locus_tag="ACGI57_001274"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFG9716359.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(5412..6731)
                     /locus_tag="ACGI57_001275"
     CDS             complement(5412..6731)
                     /locus_tag="ACGI57_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9716360.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(6772..7518)
                     /locus_tag="ACGI57_001276"
     CDS             complement(6772..7518)
                     /locus_tag="ACGI57_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFG9716361.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(7762..9771)
                     /locus_tag="ACGI57_001277"
     CDS             complement(7762..9771)
                     /locus_tag="ACGI57_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9716362.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(10209..11642)
                     /locus_tag="ACGI57_001278"
     CDS             complement(10209..11642)
                     /locus_tag="ACGI57_001278"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9716363.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(11729..13645)
                     /locus_tag="ACGI57_001279"
     CDS             complement(11729..13645)
                     /locus_tag="ACGI57_001279"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9716364.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQILITFDIESISKEGYCLETPVVVTNYSDYVDILENTQ
                     KHVNNSQELITVLA"
     gene            complement(13719..14579)
                     /gene="licT"
                     /locus_tag="ACGI57_001280"
     CDS             complement(13719..14579)
                     /gene="licT"
                     /locus_tag="ACGI57_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFG9716365.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(14930..16117)
                     /locus_tag="ACGI57_001281"
     CDS             complement(14930..16117)
                     /locus_tag="ACGI57_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9716366.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(16131..17522)
                     /locus_tag="ACGI57_001282"
     CDS             complement(16131..17522)
                     /locus_tag="ACGI57_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFG9716367.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     regulatory      complement(17666..17845)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(18117..19769)
                     /locus_tag="ACGI57_001283"
     CDS             complement(18117..19769)
                     /locus_tag="ACGI57_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9716368.1"
                     /translation="MKDKLQVLSGAMMIPVILLVVAGVFIGLGSAFANMENVSALGLS
                     GLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKKEKGWGALGGIVLFIGM
                     HTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGSFLGIFSYDCSIFGAII
                     AGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLGTAMYFIWPPVGLALSK
                     LGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVGGTMEVGGAIYEGVNNI
                     RLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLADNANKKKAISILIPAIV
                     TAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFNVSIMGESFRNMFPNLL
                     QPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSEEVKLYSKKEYRERQSV
                     DAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNCATRLRVELNSIDGFYE
                     DGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD"
     gene            complement(19789..21192)
                     /locus_tag="ACGI57_001284"
     CDS             complement(19789..21192)
                     /locus_tag="ACGI57_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9716369.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(21456..21947)
                     /locus_tag="ACGI57_001285"
     CDS             complement(21456..21947)
                     /locus_tag="ACGI57_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFG9716370.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(21973..22785)
                     /locus_tag="ACGI57_001286"
     CDS             complement(21973..22785)
                     /locus_tag="ACGI57_001286"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFG9716371.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(22798..23751)
                     /locus_tag="ACGI57_001287"
                     /pseudo
     CDS             complement(22798..23751)
                     /locus_tag="ACGI57_001287"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(23875..>24237)
                     /locus_tag="ACGI57_001288"
     CDS             complement(23875..>24237)
                     /locus_tag="ACGI57_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFG9716372.1"
                     /translation="QKLSTKLIKENDIICIEDLQVKNMIKNRKLSRSISDVSWSEFIR
                     QLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDIN
                     ASINILKEGLRLITIQNK"
BASE COUNT         7820 a         4468 c         2658 g         9291 t
ORIGIN      
        1 acataaaaaa tgtgtacttc ctatattgat agagatgatt caagttttta ttttgataca
       61 tctctatcaa tattttttta gaaaatacac atttttttca aatacttatt tttaattaaa
      121 actttaggta atctattctt tatatataat tattagactt gaaaatatct ttaaaatcaa
      181 ccaattcaat attatttctt tttaaccaca ttttaacttc cttatcacat agcattgcat
      241 gttcatatac tctctcttga ataagagaag atgattttat aagactataa tctataaatc
      301 ctggatgagt aaccatcaat gttatatctt tatctaaatt tatatatttt tcttcaaata
      361 atttaacaaa agatttattt aacttgtaaa aatcattctg aggatattta cttggcatgc
      421 taatacatga atccaaataa tagggacaac ttggtatacc gtattctttt gcagcatcac
      481 acattgctga tattgataca tcttcttcta taccatgtat atcaatatac tcaggcaatt
      541 tactcatata caacttgtaa gtttcaattt gagcacaaac ttcttcatat acttcttcat
      601 aattaaaaac ctctttacct gtcagtagtt gcgttcttct tattttgcta gaaacaaatt
      661 ttccaccttc atctaacaaa ttctttatct taatatgttc tttgcaacaa ggttctccta
      721 ttactaaatt aacatgtaaa ccaaaacata catcatcatt ttttaccatt tctatagctt
      781 gtcttgaaaa aggcatattc accatgattc cagcacttgt aacaataccg ttcttataag
      841 aagatattat ccctagtgta acaccttctg aatatcctat atcatctgcc ctaactacta
      901 atttcttcat tttatgcatc tgcctgcttt ctcaactcat caagttgcat ttccatctca
      961 tcctttactt taacaacatc cgttcctatg atgatttgaa ctgatgtatt actcaattta
     1021 attacccctt tggctcctgt ttttttaata ttatcatcat tcaaaatagt ggcatccttc
     1081 acttctaaac gtaatctcgt catacaatta ttaagagtca ctatattttc ataaccacca
     1141 caatttttaa ttatctgttt agccatatat gaaaaattac catgagaaag ttttaaattc
     1201 ttttcttttt cactaacttt atcacctatc tctatctcaa cttcacgtcc tggtgtttgc
     1261 acattatctt ttattattat atatttaaat gttacataat ataatacaaa gaatacaact
     1321 cctacaggta taactaacca cgctttatct ccataactaa aacttaagac atagtcaatt
     1381 atacttgctc cattaaatga acccaagcga gcacccaata aatatagtac aactccacct
     1441 agaccagaat aaaaagaatg aattatatat aactttggag atgcaaataa aaacgaaaat
     1501 tctattggtt cagttactgt tgccataaaa gaagtcaatg ctccagcggt cattgcacct
     1561 tttacttctt gcttcttttt atcttgtgct gttttatata tagcataaga aatagcaggt
     1621 attgaaaaca tcataattat gtaaaatcct gtccagaaga atcctgcatt tgggtctcct
     1681 gccataaatc taggaatttc tcctcttata acttctcctg ttgatgttgt atattcaccc
     1741 atttcaaata atatgtaagc atttaaaact tgatgtaacc cagttggtat tagtgcacgg
     1801 ttacagaata aaaacaagaa tattcctatt gcaccagctc ctcccaacca aaaaccaaat
     1861 tgattcattg cagcttgtgg atatatccat aataaactaa atatatatga taaaattaaa
     1921 gcaaccactg gtgatagtgt aattacacat ttttctcctg caaagaagct taaaaatgct
     1981 ggaactttaa ctttatatgt tttattgtaa gtccatgcta tacaagctcc aacaattatc
     2041 ccaccaaata cacctgcatt taatgtatca ttcaataact ctaaatttga cttaaatatc
     2101 atatatgcaa ctacggctgc aaccattaca cttgtcttgt ctttagactt tgagtaggca
     2161 ctagctgctc ctaataaaaa taagtagtcc acatttgaaa atactatatt agatatatta
     2221 gatatagctg gcacattgag catatttgga ccaaataata atcctaatat acctgctgct
     2281 ggcattacag ctattggcac cagcatagtc tttcctaaaa gtgacagttg ttgcatcatt
     2341 ttgtttaaga atctcttcat atccaatccc ctttctattt cggtttaatt ttttgtgaaa
     2401 acgatttatt cacattttat attttaaata ttatatttgt ttaaacaaat tgtcaatatt
     2461 ttttattcat tttattttta tcatttagtt taacatataa atccttttaa gtgtattgct
     2521 atatctagtc tattttttta attcattttt tttattttaa aaatatgttt aaccatgtat
     2581 ttgaaacaac ccacaaaata attctatttt gtccttttta tggttgatat gattctattt
     2641 tcactataca tgtctaaaca catttgatat aatttgttca tatcagttca ataatattac
     2701 taaagacctt taaggaggga taggggtata tggctaaaaa taaattttca gagatatatg
     2761 aagtattgaa agaggaaata cttgatggta aatatacaag taatatgatg ctgcctactg
     2821 aacttcaact tattgaaagg ttttcatgta gcagaaacac tgtacgtaga gcaatttctc
     2881 aattaaacac agaaggatat gttcaaagta taaaaggcaa gggtgttgta gtgttagaaa
     2941 atagttgttc taatgatttt tttctaaata tgcacaattt caaaggtgta gaatcgattg
     3001 tagaagataa aaaggttaat actgctacta gtgttctcca cttcagcaaa atcttaattg
     3061 ataataagtt atctaaaaag acaggtttta aagttggtag tgaagtatat taccttcaca
     3121 gactaagata tattgataac atacctaaaa ttctcgacat taactatttt ctatgtagta
     3181 tagttaaaga ccttgatgtt tctatagcac aaggctctat ttataaatat atagaagagt
     3241 gtctaggtac aaaaattgtt tcatcaagaa agatatttaa aattgaaaaa gctacagaac
     3301 ttgagttaaa aacgctacct ctaaatgatt ataattgtgt tggtgttatt aaaaatagtg
     3361 tatatactga tgatggaaag ctttttgaat atactgaatc aaagcataca cctgaaacat
     3421 ttgtattcat ggatgttact caaagatatt aaaaaaatta tgtttaatta aacataattt
     3481 ttttacgttt agacacttta tcccatacta acctcactat tctatataca attatatgtt
     3541 tatacaaata attatttatt gtatatttta gctttaatat tagtttaacg cttgacaatt
     3601 tgtttaaaca tattataatt aacttataaa ttacaggagg tatcaagtat gaaaaattgg
     3661 tggaaaaaag caactgtcta tcaaatatat ccaaaaagtt tcaaagatag caacaatgat
     3721 ggtataggtg atataaatgg tattatagaa aaattagatt atctgtattc tttaggtgtt
     3781 gatttattat ggttgactcc tatgtacgtt tctcctcaaa gagataatgg atatgatata
     3841 gaagattatt ataatataga cccaaaatat gggacaatga gtgactttga aaaactctta
     3901 aaagaagctc ataaaagaga cataaaaata atgatggata tggtcctaaa ccatacatct
     3961 actgaacata aatggtttaa agaatctaaa aaaagcaaag acaatccata tcgtgattat
     4021 tatttctgga aagatgctaa acctgatggt tctgttccta ataactggat atccagattt
     4081 agtggaactg cttggaaata tgatgaaacc actaaccaat attatttaca tttatttgaa
     4141 gaaactcaag ctgatttaaa ttgggaaaat gaaaaggttc gtgaagaatg ttacaaaata
     4201 ctagaatttt gggctgataa aggtattgat ggttttcgtt tagatgtagt caacctactc
     4261 tccaaaacac ctggattacc tgatgaccct ataacaggac caaaaggaga tggaagaact
     4321 cactacgctg atggtcctcg tatacacgaa tatttacata atatgaatca aaaagttttc
     4381 aaacctaaaa atatagttac tgtaggtgaa atgtcatcta ctacacctga agagtgtatc
     4441 aactatacta gagaaaatcg agaagaacta tctatggttt ttagcttcca tcatatgaaa
     4501 acagattata aaggaggcgc aaaatggact aatgaaatgt tctctctgga taaattaaaa
     4561 aaagcacaat ctgactttca atataaaatg tatgaaggaa aaggatggaa tgctctattt
     4621 tactctaatc acgaccaacc tagagcttta tctagatttg gagatgatag attttttcgt
     4681 caagaaagtg ctaagatgtt agccataaca ttatttggtc tacagggtac tacatacata
     4741 tatcaaggtg aagaaatagg tatgacaaat gcctacttta ctaaaataga tgaatatgat
     4801 gatgctgaaa gtaagaatgc atactatcat atgattaaat ctggtattga tgaaaaggag
     4861 gcattgctta tacttcaaca aaaatccaga gataatgccc gtactcctat gcaatgggat
     4921 aatactattt ataaggggtt ttctaatcat aaaccgtggt taaaagttaa taatgacaat
     4981 attagtgtag aaaatgagct taatgattct cgttcagtat tttatactta tcaaagatta
     5041 atcaaatatc gtaaacaata tgatattttt acagaaggta cttatagatt gttagatgaa
     5101 aatcataaaa atctatttgt ttatgaaaga gagtataaaa accaaaatct tcttgttata
     5161 agtaatttta ctcatgataa tgtagtatat aaattacctg aaacttattt gaataaatta
     5221 aactacacaa tacttgaaac caattacact agagatataa ttgaaaataa aatggaaata
     5281 aaaccttacg aaagtataat gatatattat aaataaagtt atttatgtaa aaatcggtat
     5341 aacaccctgg tgttatacca attttactaa attcaaagta atatttatag aatatctata
     5401 gattacaata attatttgac ttctacatca ttatatttct ttgaatatac tttagtcata
     5461 tagaaatata taggaactac aattatcatc ccacctaatc cccaaactat cttcataggt
     5521 tcatcaatac ctgcttttat cagtaataat atacttataa taactgcaaa tattggtata
     5581 attgctcctc caggtattct aaaatttaca tctttaccag catacttctt tctcaatatt
     5641 agtacagcta aacatgtagg aatatattga ataaatctag atacaacact tatttgagat
     5701 aatgttgtga atgaacccga ccatgctatt agtaaagttc ctatgacaga tactataatt
     5761 gctatataag gagctccatt tttattagtt tttcttatag acttaggcat taatccttgt
     5821 tcaaccaaag cagcagcaga ccttggtgta aatatggatg atgcaatatt tatacctcca
     5881 acagatacta tagttcctat tgtaattata tacccaccta taggacctaa catcttaatg
     5941 gcagcatctg caacaggagt agctgaccct gctaagccat tacccaacac ccctatactt
     6001 attcccagta taagcatata tattactgat actacaaaca ttaccattac caatgcttta
     6061 ggtacatttt tcttagggtt ctccatatct tttgctgcaa cagctaaaga ttcaaatcct
     6121 gtaaaagcat aaaataaagt tatagatact gctacaaaag ctgatgacgc actttgacct
     6181 ttttgtatta ttacaaatgg ggtaaaatta gaacccttta taaagaataa tcctacagct
     6241 ataaataaaa ctattggtaa taattttcct atagtcatga tattatttaa gtgcttagat
     6301 tgtttcatcc ccataaaatt tataatccca agtcctacaa ctaatatacc aattattata
     6361 tttttagtaa gtggttctgc tgcttgtggc catacagatg atagtgttac agcaaagaaa
     6421 tttgccatag tagcccaagc aatcatacta actatccact tcataaatcc aacttcaaat
     6481 ccaacaaaat ctccaaatgc ctcttttgca taaagatagg gccctccatc agtatcaaac
     6541 caactacctg cttcagcaaa acataaagca atagaaacag ctaaaagcat acaaaaaact
     6601 aatactaata tacttgctgg acctatatct gtataagcat caccaggtaa cccgaatata
     6661 cctgacccta ttattgcatt gattcctaat agtacaatac tccaaaaacc taacttaatg
     6721 cctttttcca ttgttattcc ccccaaagcc aacttaatat atttgtattt tttatttaga
     6781 tgcttcttta actattgcca taaatatttt tttcatatta tcataatccc tagtcatcat
     6841 ttctggatgc cattgtatcc ctattacaaa ctcacttcct tccttttcta tagcttcaat
     6901 taaaccgtct ttagatgtag ctgaaacaat atatcccggt gctactttgt tgacaactaa
     6961 gtgatgaaaa ctattaacta aaacttcttt ttctcctaat atttcatata attttgtccc
     7021 ctcctttatc tgtactgtat gtgtaggtac attagataga tgttgctgat tgtgtttaat
     7081 ataagcacct tcaattaaag ataaatcttg atacaaacta cctccttcag ttacatttat
     7141 aatttgttct cctctacata tacctaaaac tggttttttc atatctaaag catgttttaa
     7201 taatttcata tcaaaactat ctctctttgg tgatattgcc cccaacttat tatgaggttc
     7261 ttctttccat atgagtggat ttacatcttg tcccccagat agtataagcg catctatatt
     7321 agatacctgc tcctttataa tctcatcatc atatacaatc ggtacaatat atggtatagc
     7381 tttacacata actactgatt gtatataatc attatttaca taagcccttt catagccagg
     7441 aaacattcct ccttcatcaa ttagtaaatt acctgatatt cctattattg gatatttctt
     7501 tttagccaat ttcttcaccc cttaacttat ttgtgaacaa taaaaaatgc tacttctctt
     7561 aaagagcaag tagcatacca agattttatt tatttgaaca ttataattta ttttagaaat
     7621 actttttttg aatacgagta atataaataa ctttatttac ttatatttta ttttaatcct
     7681 gtttagtttg acttttttaa attgttttaa gtacaactaa taggtaaaat aggcatttag
     7741 attttccttt ttatcaacct attaaatact gtttaatata ttttttcccc ttctatttaa
     7801 ttcacttccc tttctaccag attcacatat tatcaaatcc agctctttta acatattaag
     7861 tattcccctt acctcacctt ctgttatatt gtagcctaat tcatttattg aattaacaat
     7921 accatttcta cctatatttt tattaagatt attggcattt tttaaacact ccattactaa
     7981 ctttactttc tctatagatg ttctatcctt taatatactt atttctttac taaaatcttc
     8041 agttatattt aataagttat gaggtaaatc atttattaaa ataacatcct cagaactcat
     8101 aatgttaata tacatagcag tattttttaa ttctcttata ttacctggcc attcatatgc
     8161 attaataatg tcttgaacat cttgactaat tgctctcttc cttttcataa aatgctttaa
     8221 cataacttct atatcttctt ttcgctctct taaaggtggt atatttatgg gcaatacatt
     8281 tatcctatag tataaatctg ctctaaatct atctcctttt atcatttcta ataaattttt
     8341 atttgtagcc gcaattactc taacatctat atctattaca ttttctcctc caaccctcat
     8401 tacttgattc tcttgtaata ctctaagtaa tttgctttgt aagtatatag gcatgtctcc
     8461 tatttcatcc aaaaatatag ttccattgtt agcctgttca aataatcctt ttttacctcc
     8521 ttttaaagct cctgtaaaag caccttcttc atatccaaaa agttcacttt caagtaaatt
     8581 ttctggcata gctgcacagt ttatcgctat aaatggctga ctatgtcttg gtgatgcatt
     8641 atgaatagac tgagccatga gttcttttcc cgttccactt tctcccgtta taagaattgt
     8701 ataatcagat tcagctattt tcatacctaa tttcttagtt ctttccatct tcgttgaaac
     8761 tgtttttata tcatcaaaag tatatctagc aacttgtccc ttttctctaa gcttctttga
     8821 taggttttgc tccaattttc ttatgtaagt tatttcttga atacaaaaat aatatccagt
     8881 aacccttcca aaactctcta tattatactt atttacgttt aagtacttat tattaaattt
     8941 tataacttca tcatgtgcct tactcaaact aaaaatcttt ttaatattct cattctctaa
     9001 ttcatctata tttttcccta taatattact ttgaatatct agtatatccc ctaaactatt
     9061 attgcaaata attatgtttc ccttatctga taccattgcc atacctaagt ttgataaatt
     9121 tattatagaa tttaattcat ctgcttttat agtaagttct ttatatttag tatttattcc
     9181 tgaatataaa ctaattattt catctgtata cttaattagc ctttcagtaa tttctttatc
     9241 atccagtttg agttttgcta ttatttgaat aaacgttgaa ctatcaatat acctatggcc
     9301 taaatcaata acttcttcta tatattttgg aactaaactt gcctctcctg gcgttatagc
     9361 tatcttaata tttctatatt cttcattggg attgtatggt ataaaattca gatgatttat
     9421 tcctatattg tataaattag atattgtttc caatacagta tgcttattat cgtttactac
     9481 taatacatct attccttctg gcaatgaaaa taacttatat atatcctttt gctttatgct
     9541 cctatttata gttattatct ttgagacatc ttctacatat ttttttattt ttaatactct
     9601 atcatctatc attaccaata ttatgtcatc ttttattaat tccttctctg ttattttatt
     9661 taaataataa ttttttatca ttactttatc tttaaatatc atttctagat ttttatttaa
     9721 gaaacattct aatttagaca acccatcagt tactactgct attgttttca tttgattact
     9781 ccttttaaaa tatactctac tcttttataa tatacatctg attttcactt taacagcaca
     9841 aaaaattgta catttagtat actaacatat tcctttatta gttttatgtc cctaaatatc
     9901 atagttgcaa tatctatcat atttcttaaa cttctataaa gcaaaaattg tgccaaatta
     9961 ataattttct taattttatt ctaaagttcc tcaatagtat ttacagactt agcacttata
    10021 gcttataagt ctttatatta ttaatctatt tttataggtt aattgtacaa atcatatatc
    10081 acttttaccc attaaatgca ttataaaaaa gatggaataa cgattcgtca ctccaccttt
    10141 tttgtaataa aaaattgtat atataaacac tatttactac tcttctaagt acaaacatta
    10201 tctacatact ataacctttc accattagat tctattactt gtttatacca gtcaaaactc
    10261 tttttcttaa tcctagacaa gtctccactt ccatcatcat acttatccac gtatataaac
    10321 ccatatctct tggccatctc accagtagat gcacttacta aatctataca tccccacgga
    10381 gtatatccaa ataaatcaac accatcttct acagcttcct tcatttgctc tatatgtgct
    10441 tttaaatact ctatacgata gtcatcattt atcactccat tagattcttt tttatcataa
    10501 gctcctaaac cattttcaac taccatcata ggtattctat atctatcata aatttcattt
    10561 agtgcatatc taagcccttc agggtctatt tgccatcccc attcactaga cttaagatat
    10621 ggatttttca ctccacctaa tatgttgcca cttgtattat cttctttaga atcactactt
    10681 acacatgtag acatataata actaaatgta taaaaatcta cacaaccttc ttttaaaatt
    10741 tctaaatctc ctttttccat ttttatttca atattatttt ctccaaaata tctattcata
    10801 taagttggat attcgccacg cacttgtaca tctccacaga aataattcat tactctacta
    10861 tctctttgag ttttaactgc atcatctgga ttacaagtat aaggatacat tgttgcaaat
    10921 atttgcatac atccaacttg ataatttgaa tctatttcat gagctaactt aacagcctta
    10981 gcacttgcta caaattgatg atgtaaccct tgaaaacgaa gttgaggtat atctacttgg
    11041 tttataaaat ctgtagtccc ttcatttaat attccttgtg ataagtagcc tcccataggc
    11101 attgtagctg aatttatttc attaaatgtc agccaatatt ttacttttcc tttatatctt
    11161 ttaaatattg tttcacagta attcatatac aagtctatta catctcttga aacccatcca
    11221 ttgtatttct ctgttattgc aaatggtatt tcatagtgag atattgttat taatggttct
    11281 atattatatt ttaaacattc atctatcaca ttttcataaa atttaagtcc tgcttcattt
    11341 ggttctttct ccattccagt tggaaatatt ctagtccatg ctattgaaaa tctaaatacc
    11401 ttaaatccca tttctgcaaa taaagctata tcttctttgt atctgtgata aaaatctatt
    11461 gcttcatgac ttggataaaa tgttcctttt tctataactc tcgttattct tttacttact
    11521 ttttgagaac ctccagtaca catatctgaa caagaaggac ctttcccatc taaattccat
    11581 gctccttcac attgatttgc tgctgttgct ccaccccata aaaaatcttt tctaagtgcc
    11641 ataattctac ctccaataat ttatttatag taactttatt ttaaaataaa aatacatata
    11701 tataattctt aattaataga actctctttt acgctaaaac agttatcaat tcctgactat
    11761 tatttacatg cttttgtgta ttttctaaaa tatccacata atcagaataa ttagtaacaa
    11821 ctactggtgt ttctaagcaa tatccttctt ttgaaatact ttctatgtca aaagttatta
    11881 atatctgacc ttttttaatc ttatcccctt gattaacaaa agatttgaaa tatttacctt
    11941 ccaactgaat agtatttaac cctatatgta tcaatagttc acaaccatta tcagatataa
    12001 ttcctattgc atgttttgtt ggaaataaag tcattacagt cccatcaaat ggtgccacta
    12061 cttttccttc tttaggtgtt attgctacac cttttcccaa tatacctgtt gaaaacgctg
    12121 catcttgtat ctgctccaaa gcctttactt caccttttat tgggctatat actacttctc
    12181 ttttgttttt ttctataacg ccttgcccat tatcatcttt agaaatttct actatagcat
    12241 catctttcca aaaaaataat gttaaaacaa atcctactac aatagctact attattgcta
    12301 ttatagttgc ataaacacct ttcatatctc cagttgttgg atttatataa ttaggtatac
    12361 caaatattcc aagtccaccc attacataac tttttacatt cattatcccc attacagctc
    12421 ctgccacacc accacctaca cagctatata tgaatggtgt ttttcttggt aaagttattc
    12481 catatattgc aggctctgtt acaccacata tccctgaaat aactgcaggt atacacagct
    12541 cttttatctt cttatctttt aatttaaagt acattgctgc tactactgct gtttgtgcaa
    12601 aagatggtac aaatgctccc actaatattg tgtcaaaccc catagtcata aggtttgaca
    12661 tagctattgg tactaatccc caatgtagac caaatattac aagcacttgc cacaataaac
    12721 cagttaatgc accacataaa attggtgaaa agttaaataa agctaaaaat ccatttccaa
    12781 ctatattaga agcataggta gctataggcc ctataaccat aaatcccaat ggtaaagaaa
    12841 ctaataaaac tgccattggt acaaagaaat tctttactac atctggtatt attttagcaa
    12901 acactttctc caacttagct gctaataata ctattaaaat tactggtact actgttgaag
    12961 tgtagttcat tgctattact ggtaatccta agaaagttat atatactggt gattcaaata
    13021 ttgttccagt aaatagattg tataaaggtt gacctgtaga tgctagagta tctaattgaa
    13081 tacctgaata gcataaaact gcacctataa gcattccaat aaatggtttt aatttgaatt
    13141 tcttagcagc tgtatatcca agaattattg gcatatactg gaacattgca tctccaattg
    13201 catttaacat aatatatgca ccatcagttt ctccatacaa acccaatgct gtaaatactg
    13261 cattaaatcc ttttatcata ccagcagcac acagaactcc taatattggt tgaaaaactc
    13321 ctgatattat gtctataaac ttattaagta atccttttgg cgcatctgag tctgaattat
    13381 ctattgtatc tcctcctaat ccagccaaat cgcacacaac ttcataaaca tctggaacat
    13441 gattaccaat aactacttgg tattgacctg cacttttcat tacagtaact ataccatcca
    13501 ttttttttag cacatcatca tttgctattg actcatcttt taatttaaaa cgtagcctcg
    13561 ttatacagtg agataaactg ctaatattat cctttcctcc tacattcgca atgatttctt
    13621 ttgatagttg ttcatacttt ctcatttttt ctcctccatt ttctttttga aatatgaagg
    13681 tttggccaac ttaatgtcac cacccaatat atataaacct aattattagg atttatatct
    13741 gacttaatac ttttacttac cactctttca acgtgaatag ttaagtataa tttttcctca
    13801 tctgttaaat cgtagttgta ttttttatgt ataaattcat ccaacttttc tatgaattta
    13861 taagcatttt tatatttaac ttttattaca tttaataaat catcttcatt attttcatgt
    13921 aaattattac taacaattcg ttctgcaaaa tactttaagt gagtaataaa tctatgataa
    13981 taaactgaat cttcatcaaa tactattctg taatggtatt taaccatatt tgaaatttct
    14041 tgtattattt gtgtaatctc atatatagtt tttatacttt gatccatttg tgcattaact
    14101 atatgcagtg ctataaatcc agcttcatct tctgatagtc taatttcaaa ttttttttct
    14161 atataatcca atgcctctaa ccctatttta aattcactct tataaaatct ttttatatcc
    14221 caaagtaata cattttttac acttactcct tcacgagctc tttctattgc agtatacata
    14281 tggtctgtta aagaaatata aatactatca tttaatcttt tccctagttt ttctttagca
    14341 tataaaatta tttcatctga taattccata tatcttatag gaatatctgc tattagttct
    14401 ttaaatttat cagatatatt tgggtctgaa agtttgaata tcttatcaat tttatccttt
    14461 gatatatgct ctcctgtacg ttttttataa gctaaacctc tccccataac tactatttcc
    14521 ttatgatttt catcagttgt aataattaca ttattattga gtattttttc aataatcatt
    14581 gtgatgcccc ctttattagc attaagttaa aataaatctt ttttaattat ttagaatatt
    14641 taaaatgtaa tttattacat aaaaaaaaac ctatttatta cacattaaat agactcccat
    14701 gagtcctgag taataaatag gtttagcttg tatttaaaca atcactatcc gtttgtattt
    14761 tattttaatt attatttatt atatgttagc aaatttattt tttagtgtca actttttctc
    14821 aatattttaa ttttaaataa attatataaa ttgaatcttc aaagtactat ttttatcaat
    14881 aaacaaaata aaaaaggtat tactttcttt aagtaatacc catttatttt taatttaagt
    14941 ggtttttctt aatctcatca agtagtccat cttctaaata aatttctaat gcacatccta
    15001 ccattgcctt tactgctttg tgaagtttat cataagcttc ctcagaattt gcataattta
    15061 gaaattcttt ttcatgaaca tataccattg gacttatatc taaggctatt tctgtataca
    15121 tagtagggca aacttgactt acattaccta tatctgtaga acctactgga ccatctttac
    15181 cttctaatat atctgttata cctgcctcaa tcaaattatt ctttgttatc ttttgaagta
    15241 ctttgttatt tattatgttg tcaaaagaat tttcaaagta tctataagaa agcttagctc
    15301 ctgtcattaa ctctgcacct tttgcacagt ttataacttt tttagttact tcatctaaat
    15361 aacttctttc agcagctctt acgaaaaact tacaagctgc tttttctgga actatatttg
    15421 gagcctctcc tccatttgaa actataccat gtattctaac atctgatttt acatgttgtc
    15481 ttagtgcatt tatcccagca aaagtaagtt gtactgcatc tagtgcattg actcccatat
    15541 gagggtgact agctgcatgt gctgataccc cttcaaaact aaactctaat gaatctatcg
    15601 ccaaagcctt acagtttata ttattaaaag cttctatatg catttgataa cttacatcaa
    15661 catcatcaaa agctcctgcc ttaactaaat cacattttcc tcctgttgtt tcttcagctg
    15721 gtgttcctat tagtacaatt tttcctttaa aattttcttt taacttactt aatactatac
    15781 aagctcctag tgtagatgct gcaatccagt tatgtccaca agcatgagct ggttctttat
    15841 tttctccata tccaggaagt gcatcatact cagctaaaaa tgctatggta ggtccatcat
    15901 tatctcctaa ttcagctcta aaagcagtct ccatattaca atatggataa actgtatcaa
    15961 atccatattc cttcattttt tctactaagt attttgatga tatgtattct tcttccccta
    16021 attcaggatt gttaaatata aaattacaaa cttcttcaat ttcttttcca agttcattct
    16081 ctatctcata agctctcttc tttatactct ctatcataat ttacctccta ctagaatcct
    16141 agatataatc ctattgtcaa aaatgccatt acaagaattg ttaatactac cattaaagga
    16201 attacgaatc taacccactt tgaccaagga atattggcta ctgctagtgc tcccattaaa
    16261 acacctgatg taggtgttat caagttagtt acacctatac caaattgata agctgtaact
    16321 atcatttgtc ttgatacatc tactaagtcg ccaagtggtg caagtacagg tattgtaagt
    16381 gatgcaagcc ctgatgctga tggtattaaa aatgctatta atccttctat taagaaagtt
    16441 aaatttataa atatagtttt tggtaatcca tttaataaat ttgctgcaga atttaaaatt
    16501 gtatctatta taaatccatt ttctgctatt attacgattc cacgtgcaaa tgctatacac
    16561 aatcctgctg taattaaatc tcctgctcca cttataaatg aattaactat ctcatcttgt
    16621 ttaagccctg atgctattcc tgatattata cctatagctg taaatatcat tgcaatttca
    16681 gttatgtacc attctagtct taaaactcca tatatcataa tcgccatacc tattgtgaaa
    16741 atggctatta ccatggcttc tctttttgta aattccttta tatttgtatc actattaacc
    16801 attaattctt gatttagtga tatatcatgt actaaagatt ttttagggtc ttttttaact
    16861 ttattggcat acatcataac aaatcctata gatatagcca tatatattat aaacataaca
    16921 actctaaact ctattccaga acctggtact atttgtgata atgcttgtgc tattcctaca
    16981 gagaaaggat tcgtagtagc tgctccaaat ccagctgttg ctcctataaa tactgtggcc
    17041 actgctgtca atgaatcata tcccatactg gtcataagtg gaataaatat catataaaaa
    17101 ggtagtgtct cttcactcat tccaaacgtc gtcccaccta gtccaaatag aatcattgaa
    17161 atcggtatta ttaaaaattg aaacttcttc attttattta cagtatgagc tataacagct
    17221 tctatagctc ctgttttatt aactatccca aaagctcctc caactattaa aacaaatcca
    17281 actacttcag ctgcatctat aaacccgttt ataggtgctg taaatacatc agtaattcct
    17341 tgtggatttg atggtgcttt ttcataagtt ccagctacaa caactgacct tccgtctata
    17401 tctactcttt caaaatttcc acttggcact atccaagtaa gaattgccat taacactatt
    17461 agcaaaaata ttattgtaag tgtctgtggc atagtaaact ttttcttttt taaactgctc
    17521 atttttaatc tccccacttt ttaaattaaa tattttatta ttgcatccct ttatcctatt
    17581 ttgtatagat gtaaaaaagc tcaacatgga tacatgttga gctaaatgtg ctaaatacaa
    17641 catacctgca tcttcccttt atcttgttta agatagctct ccatcaaaat tcgggataat
    17701 tttgatgaca gtactgtacc tattaaatac agtcccagca ctagcttcgg acactagcac
    17761 ttcggcactt actcctttta tattgcctca ttggtttcca acctcttcaa tattaatctt
    17821 agtaaatgca acctctacct caatctgttt gttagatgag gtaattctta ttaattttta
    17881 ttaattcaaa gtaattgtag cataatatgt cgagattgta aatattttca gtataataat
    17941 tcttacttta tgatttcatt ccatttttca caaataattt tttctccata taatttaaat
    18001 actgtagtat attttaaata cacacaaact ttccacaaat tttaaattat tataatacct
    18061 ttataataaa aaataaaagc tatgtaaact tatcaattta cacagcttta tatacattaa
    18121 tctactccaa gtacagcttt aagctttgat gctattgtaa taactcgtgg accaaatata
    18181 acttgaactg agtttttctt tttaacaact ccacttgcac ccaattcatt tacccagaag
    18241 ccatcttcat agaatccatc aattgaattt agctctaccc taagtctagt tgcacagttt
    18301 gtaacatttt ttatattatt acttcctcct aaaccttcta ttatagaatg tacaagcttt
    18361 gtatctttat catctacttc ctcttctact gcattgttgt atgctgtttt accagcatct
    18421 acagattgtc tttctctata ttctttctta ctataaagct taacttcttc tgaacttctt
    18481 cctggtgttt ttatatcaaa cttcttgatt gcccatgtaa atattatata agtaactata
    18541 aaaaatattg gtatcaaaaa taataatggt aacgcatcca ctttttgtgg ttgtaataaa
    18601 ttaggaaaca tatttctaaa tgattctccc attatagaaa cattaaacat ttctgtaaat
    18661 acatacgtca atcctgccag tggtacatat gctacaaaat acaataatgg tgcaacaaat
    18721 aggaatgtat actctattgg ttccgttacc cctaccaaca tggctgttac tattgctggt
    18781 atcaatatac ttattgcttt tttcttatta gcattatcag ccaacttata catagctaaa
    18841 gctgctgctg gtaatattgc aaaatgtatt aataaacgac ctgttgtgaa gttacgagtt
    18901 atataagata gtgtatctgg gtctcccatt tgagctaatc ttatattatt aacaccctcg
    18961 taaattgctc cacctacttc cattgttcca cctactcttg tatattctat agggaatgca
    19021 attaagtgat gtataccaaa tggtaataat gctttatcca ttgctccaaa agtaaatgtt
    19081 cctaataatc cactagaacc tatccaatta ccaagtttag ataatgccaa ccctactggt
    19141 ggccatataa agtacattgc tgtacccaat actattgatg ctataaacat cataatcatt
    19201 gaaaatcttg gacctgagaa aaaagataat gcactaggaa gttctatgtc aacaaatttg
    19261 ttatgtactg cactagctac aaatccagct attatagctc caaatatact acaatcatat
    19321 gaaaagattc ctaagaaaga accatataaa gaacttcgac tatatgcttc tgcctctcct
    19381 aatccagatg ccataaacgc ttctgctgta actgtatctg aatttatacc atttgctgct
    19441 aacaatgtac ttataactgt gtgcattcct ataaataaaa caattcctcc taatgcaccc
    19501 catccttttt cctttttaga taatccaaag gctatacctg taacaaagaa tattggtaag
    19561 tatctcatta ccataaatcc taaatcgttt attactttaa agaatacatt tataaatgac
    19621 ccttctttta ttagtccgct aagccctaat gcagaaacgt tttccatatt tgcaaaagct
    19681 gaccctaatc ctataaatac tcctgctaca acaagtaata taacaggtat catcattgct
    19741 ccagaaagta cttgtaattt atccttcata gtattatttc ctccattttt attgaaatgc
    19801 caggctatat aatgcatata tatatacttc tattgctttg tataatgtat ctatacttat
    19861 attttcattt gcctgatgtg aatttggatt atccccctct attgtgggtc caaatgcaac
    19921 tccatttttt aacactcttg cataagatgc agctcctttt gtaaaaagtt cggcttcttt
    19981 tccaaaacct tgtttatatg catttgataa tttatttatt aattcactat ctggttttac
    20041 atacagtaat tttaaatcct tatatatatc aactattaaa ttttcttttt ttccaatttc
    20101 attaattcta ttagttataa actcccaact tataccctta ggatatctaa agtctatttt
    20161 catattcaat tcatttttct ctaagaagat actcattata catattgtag aaactcccat
    20221 atctacatct tctttataca tccctaattt ttttccatga ttatcatctg taaagtatga
    20281 atttaaaata tcccttatta taacgccttt atcatttagt tttttaattt tctctaaatc
    20341 ttttgctaaa ctaaatgcac agttatatga cctgtgtgga tttctcgata aagccctttc
    20401 tcctgtatat cttactaata ctttaccttc atctaattct tctatctctg tataataagc
    20461 taatgattct actaaatctt tcttatcatt agttttaaaa acagcctcaa ttttgtcaca
    20521 tacaaatcca tcttctttgt ttgactttat atcaactaaa ttgtgatttc taaatttatc
    20581 tttatctatt ttctttctta gattgaagta taaaatacct ttttctccat ttactattgg
    20641 gaagtttcca tctggagaaa atgcaaattt tggctgttca ttgacactaa agtaatgctc
    20701 catacattcc caagtcgtct cttcagctcc acccactatc aatctaatct ttctcttcgg
    20761 cttttcatta agttctctta agaaaagtaa tgcatataaa atccctatca aaggtccttt
    20821 gttgtcatta actcctctac cataaagata gttatctttt tgacatactt taaaaggttt
    20881 actcttccaa agttctttat tatatattgg caccacatct atatgattaa gcacccctac
    20941 tacttcttct ccttctccat attctatatg aattgcatat ccatcaaagt cttttacaac
    21001 aaaatctttg tcttttgcta tttgaataag cttatcgaag gcatttctta tttctatccc
    21061 aaatggttga tttatatctg ttgtactttc atctctaatt gaaggtattt caactaaact
    21121 tctaatatca tttatgatat tttcttgatt ggattttaaa taatcaataa ttagttcttt
    21181 gtttactatc atcttaatta tttatcaccc ctttatatta attttacaaa gcatttcagt
    21241 tcatatctac attataaatt ttgaatatta ttttttcaat atttcaaata cttctctaag
    21301 ttaccataat gatacggtaa acaatcaaca gattatgaca aaatcatact atacctatta
    21361 ctaatatttt aacttatcta ttacgtcttt tttaatgtaa gtttattcca aaaatacata
    21421 aaaaaagatg tagccttagc tacatcttct tatatctatt caacttccat tattatgcta
    21481 ttaatatcta catttccagg ttctatccta tcttttactg ttctcttaac agaatcagca
    21541 tcatccatta taactacagg agttattata tttaaatcat ttgacgttat aaaatctaaa
    21601 tctacttcca ttattaaatc tttagctttt accttatctc ctgcctttac cttaacatca
    21661 aacggctttc cttctagtga cattgtctct aaacctacat gtattaatat atttgtatta
    21721 tcattactct taatcattat tgcatgcttt gtatcaaata cattgactat ttcaccatca
    21781 acaggagaat ataacttacc ttcacttggt attattgcta ctccatctcc catcatcttt
    21841 tgggcaaaaa ctgcatctgg aacatcttct aagttcacta aatttccctt aaaagggcta
    21901 tatattttct caatcttatt ttcttttttc ttaaatatat taaacataac acactctcca
    21961 cattataaaa ttttatgata atatttttct taaagctttt gctacaccat catttacatt
    22021 agtatcagtt acaaaatctg ccatagattt tacttcatct gtagcatttc ccatagcaat
    22081 accaagacct gcaaatttaa tcatggatat atcattttca ttatcaccta tacaaataac
    22141 ttctttgcta tctataccta atatatcaca aaatttttta actgcaatac cttttgaagt
    22201 acctttactc ataacttcaa agtttatctt tccagaactt actacttcta aatcttcttg
    22261 ttttttaaat tcttcctttg cttctctcaa agaatctacg ttttctgaga aaagtataca
    22321 ttttaaaatt tcattatcag ctttttctaa taaagtttca caacttgcat tttctattat
    22381 ttcaattctg tcttcttctt tatttttact atttattaat ctatatggat gttcttcacc
    22441 tatttcattg ttggatataa ctattttaca gcctttaaaa tgtttgttta aattatactt
    22501 ttcacctatt gtatagattt tctttagagc ttctttgctc aaaaccttct tatactcata
    22561 tcccagtaat ttaaaatatg tcccattagt tgatataata tatatatcat cccctaaaac
    22621 acttgtaaaa tatttcatag cattataagg tcttcccgta actaaggcta ccttgatacc
    22681 cttttccatg gcttctttta gtgctttaat attttcttct gatacttcaa atcctttacc
    22741 catcaaagta ccatccatat cagtacaaat taatttatat cgcatttgta tcacatccta
    22801 gtaagatttt tctaatattt caacaaggtc ttctttcttt ccaaaaggta aaaatcctaa
    22861 aactggacag cttgttattt gttctaattg ttcctttgtt acattaaact cagacatttt
    22921 tgtcttaagc cccactctct ttaaaaaact ttctatttca tttcctaaag ttttagctgc
    22981 ttcatcatct tccacttttt ctaattcttg gtttagtatt ctcgctactt tggcaaattt
    23041 ctctatatta tccttataga acttttctgc aaattttggg aacactatag ccaaagcctc
    23101 accatgagga atatgtgtaa ctcctcctat tatttcactt agtggatgtg gtgctgctgc
    23161 tccagcattt gccagtgaac ttcctcctaa tgtatctgct actgctaact ttgttctgta
    23221 ttctatatta tttttatctt ccattacttt aggtagatag ttaatgacta attccataga
    23281 tttttcagac attatctctg taaaatcatt agcatttgga tttatataac tttcaaatgc
    23341 atgagtaaaa gcatcaaatg ctgttgatgc agtaattctc tctggcagag ttaacataag
    23401 ctctgggtca actatacatt cttttgagaa acaattttga tgaaatattg tatttttctc
    23461 gcttcctctc gatattactg ctgcttgtgt aacttggctt cctgttccag aagtagttga
    23521 tacagctatt aatggtaatt ctttttcact taccttatcg tatattttaa atggatttgt
    23581 atatgtacta aacatataat cccaatctat tttttctaaa ccaaaagtca atgctaatac
    23641 ttttgcagta tctatacttg aacctcctcc aacagctaaa acaacgtcta cagattcttt
    23701 tttagctaat tcaaaccctt cgtttattaa ttctacagtt gggtttggct ctactctctg
    23761 aacctcccat gactaaagtc acagggttcc tgcttcacag aattttgcat actaaaatat
    23821 gtcttactaa ttctccacag gccatccccg tagttcctac ggttcttaca taagttattt
    23881 attttgaatt gttattagtc ttaatccttc ttttaatata tttatacttg catttatatc
    23941 tctatcgtga gtttcattac aactaggaca aatccattct cttatattta agtccttcac
    24001 ttcctcgttt ttatacccac atttattgca tatttgtgaa cttgcaaaga atttacctac
    24061 ctttacaatt tgtctaccat gccaattagc cttgtattct aattgacgaa taaattctga
    24121 ccatgataca tctgaaattg agcgagatag tttacgattt ttaatcatat tctttacttg
    24181 taaatcttct atgcaaataa tatcattctc ttttattaat ttagtagata atttttg
//
LOCUS       DAYAOX010000041        24108 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.041, whole genome shotgun sequence.
ACCESSION   DAYAOX010000041 DAYAOX010000000
VERSION     DAYAOX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.041"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            401..3304
                     /locus_tag="ACGI57_001289"
     CDS             401..3304
                     /locus_tag="ACGI57_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9716373.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3337..3783
                     /locus_tag="ACGI57_001290"
     CDS             3337..3783
                     /locus_tag="ACGI57_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9716374.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3906..4208
                     /locus_tag="ACGI57_001291"
     CDS             3906..4208
                     /locus_tag="ACGI57_001291"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9716375.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4389..5795
                     /locus_tag="ACGI57_001292"
     CDS             4389..5795
                     /locus_tag="ACGI57_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFG9716376.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            6139..7197
                     /locus_tag="ACGI57_001293"
     CDS             6139..7197
                     /locus_tag="ACGI57_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFG9716377.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVNIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7296..7982
                     /gene="araD"
                     /locus_tag="ACGI57_001294"
     CDS             7296..7982
                     /gene="araD"
                     /locus_tag="ACGI57_001294"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFG9716378.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            8000..8647
                     /locus_tag="ACGI57_001295"
     CDS             8000..8647
                     /locus_tag="ACGI57_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFG9716379.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8820..10187)
                     /locus_tag="ACGI57_001296"
     CDS             complement(8820..10187)
                     /locus_tag="ACGI57_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFG9716380.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10377..10465)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10893..13013
                     /locus_tag="ACGI57_001297"
     CDS             10893..13013
                     /locus_tag="ACGI57_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFG9716381.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRLFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            13117..14370
                     /locus_tag="ACGI57_001298"
     CDS             13117..14370
                     /locus_tag="ACGI57_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HFG9716382.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVIAMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(14524..15585)
                     /gene="ytvI"
                     /locus_tag="ACGI57_001299"
     CDS             complement(14524..15585)
                     /gene="ytvI"
                     /locus_tag="ACGI57_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HFG9716383.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            15859..16599
                     /locus_tag="ACGI57_001300"
     CDS             15859..16599
                     /locus_tag="ACGI57_001300"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HFG9716384.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            16611..18389
                     /locus_tag="ACGI57_001301"
     CDS             16611..18389
                     /locus_tag="ACGI57_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9716385.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTGIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            18556..19473
                     /gene="pfkB"
                     /locus_tag="ACGI57_001302"
     CDS             18556..19473
                     /gene="pfkB"
                     /locus_tag="ACGI57_001302"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFG9716386.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     IFKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            19510..21429
                     /locus_tag="ACGI57_001303"
     CDS             19510..21429
                     /locus_tag="ACGI57_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFG9716387.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            22025..23551
                     /locus_tag="ACGI57_001304"
     CDS             22025..23551
                     /locus_tag="ACGI57_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9716388.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            23579..>24108
                     /locus_tag="ACGI57_001305"
     CDS             23579..>24108
                     /locus_tag="ACGI57_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716389.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIP"
BASE COUNT         9662 a         2526 c         4227 g         7693 t
ORIGIN      
        1 aatcatgtta aaactattta tatagctaaa atcttaccta tataagtatg tatactatca
       61 tataaaaata aaatcttaaa atagaaaggg ttaaaaacac agtttattat aatttttatt
      121 gttgagtaga tacgtactat tatatatgat tgagcaagtt tttcaatcat aaatttataa
      181 agtattttgc attaaaattt aaacaaagaa ttaaattatt taagattatg aataactaat
      241 acatttagta aattactaaa tatacaaaat gtaaatactt atcaaaatgt ataaatactt
      301 atgacatcta gttacttaca gggtttaagt agaaaaaaat atcaaagtaa gtatggcatg
      361 atagttgctt atatatttat caagaaagga gttattaagc atggagttga gagataaact
      421 tgtaaacatc ataaatacag aaaataaaaa agatccatta acagatgttc aaatagcaaa
      481 gtttttaagt acaactagag agaacataac taatttgaga aaagaactta atataagtaa
      541 ttcaaggcaa agaagatatc cttatttaaa atcagcaatt tcggcaatat tacaaaagaa
      601 taagaatatt tctatatcag aaattacaag ggaattgatg acagaaggct ttaatatatc
      661 gagaagagtt gtggaggaac ttttacctaa agaaagttta gaaatggatg tagtagagga
      721 agatgaagaa gaatcgaatg acccatttga aactctgata ggtaataaag ggagtcttag
      781 aaatgctgta gagcaggcta aatctgctat attgtatcca ccaaaagggt taccaacgtt
      841 gattattggg gaaagtggag ttggtaagag tttattttct agacatatgt atgagtttgc
      901 aaagcaaaaa aagatagtaa aagagagctc caattttgtt gtatttaact gtgctgatta
      961 tagtgacaat ccacaattat tattatcttt gttatttgga tataaaaaag gtgcatttac
     1021 aggtgctgaa tatgatacac caggtcttgt agaagaagct gatgaaggag ttttattttt
     1081 ggatgaaata catagattgc ctccaaaagg tcaagaaata ctattttcta tcttagatag
     1141 gggaaagttt aggagattag gtgaaactaa tgctgaaaga aaagtaagta ttatgttgat
     1201 tggggcaact actgaaaatg tagagactaa tttattatta acattcagaa gaaggatacc
     1261 aatgctaata actttgccac cattacatga tagattacta aaagagaaaa tagaccttat
     1321 atacaatatc tttcaacaag agtgtaatag aataaatgca aaaatatttg tagataaaaa
     1381 tgtgattgaa atacttgcac taaaaaagtt tagtggaaat ataggacaat tacaaaatat
     1441 gattcaggtt ctttgtgcaa gagcttttat gaagtttata aattccaaag atgaagatag
     1501 tgaatctata gtagtagtag atataaatga agttttaaag cttaaagatt cgtttaaaga
     1561 tgctgccttt caagagattg aatatacaga gataagaaaa tatttgaaaa atgctatatt
     1621 tataccattt aatttagaag aatcatcagt aaaaggaagt cttttaagtg atgaatatat
     1681 attaccagag gacatataca aaacaattga aaagaaatat tatgacctaa aatccctaga
     1741 cattgaagat atagatatag agaatatttt atggactttt attttaaata gatttgctga
     1801 cttaaagttt aatcttgata gtaatgaaga aattttttca atgagtgatt taaatagttt
     1861 tgtaagtgat agtttagtta aactggtaaa agaacttatg aatgatatag ttgaaaaaaa
     1921 tatgaattta gaagtaaata aaaatatgtt caaatatcta gcaatacact tagaagaagc
     1981 tataaaaaga attaagctaa atcaaaagat aataaatgta aatttagaaa aaataaagat
     2041 tgacttttca aaagaatatg aaattagtaa aagttttgca ataaaaattg aggaatcaat
     2101 tggtattaag atttcagatg atgaaattgg atttataacg ctctacataa agtctgctct
     2161 aaaaaatgaa gtgaaaaaag ataaagtagg gttaataata atttctcatg gacgtatagc
     2221 tacagagact gtaaatgtag ttaaagaact tttaggtgta aagtttcctg tggctataga
     2281 tatgccttta gatgaaaaac caataaatat atacaataag gcagtagaat tgtcaaaaat
     2341 aatagaccaa gggaaaggca tattgttttt ggtggatatt ggttcactta caaacatagg
     2401 tcaaatagta aataagagaa ccaatataag tactaaaact attgatagag tagacctcct
     2461 aatggcacta gaagcaacta gaaaagtttc tatgggagag gaagaacttg atgaaatatt
     2521 tttttctttg ctaaaagata ggatgggata taactatgaa ttagaaaaac acatagataa
     2581 atcaaatgca attataacat tatgtcttac tggagaagga actgctaagt atataagtaa
     2641 aacgttggaa gaaaagtatg ataacactaa atgttatcaa atgagtgcat tggatgaaag
     2701 tttattttgt aaaattgaaa atctaaaaga gacaaacaat atattagcta ttgttgggac
     2761 aataaaccct aagataccag gtataaattt tataccctat aataaagaac ttttcaaaaa
     2821 tttggatatc tatctatcaa aatcaaaaca acaagatgac agaccaatta aatcgtatga
     2881 aagaatgctt gatgaggatt tagtgatttt tgaaccagat atatatttta aaaaggattt
     2941 attagaatat gtatgttcaa ttcttataaa taaagaatat gtagaaaaag attacttaga
     3001 ttctgtttta catagagaag aaatgcttcc aacatattct aaaggagcta taggtgtgcc
     3061 tcatggagat tcttctactg taaatagtac aaggtttgtt tttgttaaac taaagaatcc
     3121 tattgattgg ggtgtaggta atgtaaattt catatttatg ccagtttttc aggctaatga
     3181 taaagagatt gttaaaaatg ttcttgagat actaaaagat gcagaattta tgaacaatgc
     3241 caataagtgt tttgataaag atgcttttag aaggattata tttgataaat ttaaacatct
     3301 gtagccatag caaaaaaatt gagaggagtt agagtaatga atgtagattt aatatcattg
     3361 ggtttagaat ctaatgatta tgaaagtgtt attgaagaat taggaagtat aatgtgtaaa
     3421 aaagaatatg taaaagaaac atacataaat gctgtcttgg aaagagaaag gacattacca
     3481 acaggtctag atattggaga catgtgtgtt gctattccac atacagattc aaagcatgta
     3541 aatgagtcta atgtagctgt agggatattg aaaaatcctg taaagtttaa ttcaatgata
     3601 gacccaaaag atagacttga tgtagaactt gtatttttat tagcagtaaa gaacccagac
     3661 tcacaagtaa aactgttaaa agatttaatg tcagtttttc aaaatataaa gttattaaaa
     3721 aatataaaaa acgcatcaac aaaagaagaa gtagctaaat tattggattt tatagaaatt
     3781 taatgtagga gaattttata aataaaaaat taaaatttaa acaatcaaag taagtattat
     3841 ttttttaata ggtttaatct tatataaaat aaaaatttag agtgaaaaat taggaggaaa
     3901 ttattatgaa acaatttaat gtattatcag tttgtggttc agggacagtt acatcatcta
     3961 tggtagcagg taaattaaaa gaagtactag gtaataaggg gatttctatt actacaacag
     4021 aagctagacc aacagaagca ctaaatttag cacaatcagg aagatttgac tttataacat
     4081 ttacgagtcc acttcaaaca ggcgattatg gaataccaac tataaatgca tttgcttgcc
     4141 taactggtat aggggaagag gcattctttg aagaagtttt gactgttatt gaagggatag
     4201 ataagtaagt tttggagacg tgaatttata gtatttaaat caatgattta gatactataa
     4261 taagctaata ttaactaaat atttgtggaa aaaacatttt atatgttgtc ataaattaaa
     4321 aaattataaa aatagtcact taagtattaa attaagctta taaatattta taagtaggag
     4381 ggaaaaagat gttagaattt atgaaatcta taatcgatac atttggttct gcaattatag
     4441 ttccaattat aatattcata atcgcaaaga tatttaaagt aactactaag aaatcgtttc
     4501 tttctgctgt gtatgcagga gttgctttac aaggttttac actgatttta aattcattta
     4561 caccagtaat aacaccagtt atcaatagga tggttgagag tacaggagta aacctaccag
     4621 tttttgatgt tggttggcaa gcaacatcat tggttgcatt ttcaacaagt gcaggtatga
     4681 tatatttagg tcttggtata ttattacaaa caattttatt tttgataaaa tggacagatg
     4741 tgtttcaacc atcagattta tggaataact attcttatat ggtatgggga gcaatggtta
     4801 taggagtaac aggaaatttt cctttaggta tagcatgtat ggttttatta aacctatata
     4861 gtttattaat atcagagtta gttgcaaaga gatggtctag ttactataga tatcctaatt
     4921 gtacaataat agctatgcat aatgttgagg cttctgtgtt tgcagtattt gcagacccta
     4981 tatacaataa attgggatta aataaaataa aattaaatcc aaaagaatta gagaagaaat
     5041 tagggttttt aggtgaacca attactttag gtcttttttt aggtatgttt ataggtatat
     5101 taggtaatat gacgagaata aatactatgg aagcttgggg agaaataatg aaagtaggta
     5161 tatctacaag tgcagtgatg gcaattttcc ctaaagtagc aagtatgttt gctcaagcat
     5221 ttgcacctat aacagaagca gctagaaaga ttatgcaaaa agctggaaac cgtgagtggt
     5281 acatagcagt aaatgatgct gttggatatg gtgaacctgc aactttaata tcaggtctta
     5341 tattaattcc aataatgctt gtaatagcta tggtacttcc tggtaataag gtattaccag
     5401 ttgtggattt attggcaatt ccatatatgg ttcaaggttt agttgccata cataatggta
     5461 atataccaaa agtattagtt tcaggtataa tttggtttgg attaggatta tatgtttgta
     5521 catctacagc accattattt acagatatgg ctacaaatat aggtgtagct atacctgcag
     5581 gagcaatgtt aataacaagt tttaatatac taggaaaacc tttgatggga ttagttttct
     5641 ttgcatttct aagtgcaaat ccaatttata taggtctttc agttgtaatt tactttgttc
     5701 tatgggcatt gtttagaaag aacaaaacaa gtattcttga ttatttagaa aaacaagcac
     5761 ttaaaaatgt agaagaagag cctgtagcag tttaagttta taaaaatgaa ataaaaataa
     5821 ttaaaggagt ttataattga ttttatataa gtatcagata atacctgaga aatatttaaa
     5881 tgaaatatag acttcttttt atttgataat atttgtaatt ataatgcatt gtatttttat
     5941 agttgagttt tctgtataac ctgctattta aaacatactc ttttatattt atacagtaat
     6001 gtggtttaat tttataaaat gtaatctcta ctggtaaaag actttgtata taaggcattt
     6061 gcctgaataa accatttatc ttttgtatgg catagatatt gctatataga agtatatagc
     6121 atgaaaggag atatttatat gggaaataaa atgagagcat cagtattgta taatgtaggt
     6181 gatgtcagat atgaaatggt aaatatacca gaaataacag atacgcaagt gcttgtaaat
     6241 gtaaaatatg ttgggatatg tggttcagat ttacctaggt ctatggtaag tggtttgagt
     6301 ggaaatacaa aatatccatt gattttaggg catgaatttt ctggtgaagt tgttaaaatt
     6361 ggagaaaaag taaagcatat taatgtagga gatagagtag ctgttgcacc attagtacct
     6421 tgtggaaaat gtgattattg taatgaaggt aattttggct tgtgtgatga ttacaatata
     6481 attggaacaa gagtaaatgg agcgtttgca gaatatgtaa gagttccaga agaacatatt
     6541 ttaaaactac cagatacatt ggattatgaa actgctgccg gaattgaacc agcaactata
     6601 gcttatcatg ggatttcaaa gtctaatatt agagtaggag atagtgtagt tgtgttagga
     6661 tgtggtccaa taggtcagtt tgttatacaa tgggcaaagg tgtttggtgc atctaagatt
     6721 atagcagttg atatttttga tgaaaaatta gaactttcta aacttctagg agcaaactat
     6781 atacttaact caaaagaagt aaatgtaatc aaagaaataa aaaaaataac aaatggtggt
     6841 gctgatgttg taatagaaac tgctggtagc cgatttacac aagaacaatc tctatttgta
     6901 gcaaaaaaga gaggaaatat agtgtttgta ggtatatcac acacagaatt accacttagt
     6961 gcagacgcta ctgagtgtat attacgtgga gaacttacct tgaaaggttc ttggaattca
     7021 tatacaagcc cttatcctgg aagagcatgg actgcaacat tggattttat ggaaaaaggt
     7081 gacattattt ttaaacccat gatttcagat aaaataggtc ttaatgaagt gggtgacttt
     7141 ttaagtaaaa tgagcaagag agaaattaat ttcaataaaa tcctagtaga aatataaaaa
     7201 tttaataaaa aattctaaaa gcaattaaat tttataacta tgacattaaa taaaataaaa
     7261 ataattttct taaataaaat ataggaggaa gaaaagtgtt agaaaattta aaagaagaag
     7321 tattaaaagc aaacttagaa ttacctaaga aaaagctaat tacttatact tggggaaatg
     7381 taagtgggat agatagagaa aaaggattag tggtgataaa gccaagtggt gtagagtata
     7441 gtaatatgac tgtagaagat atggttgtag ttgatttaga gggaaatata gttgatggca
     7501 aattaagacc ttcttcagat accccgactc atctagtttt atataaagag tttgaagaat
     7561 tggggggaat agtacataca cattcaagtt gggcaacaat ttgggctcaa ataggaaaat
     7621 ctataccatc ttgtggtact actcatgcag attattttta tggaagtata ccatgtacca
     7681 gaaaaatgac tagagaagaa atttgcaatg aatacgaaaa ggaaactgga aatgtaatca
     7741 ttgaagaatt tatgggaaga aatccaattc attgtccagg aataattgta aatgaccacg
     7801 gaccttttac atggggaaaa gatgcaaatg aggctgtaca caatgcagta gttttagaag
     7861 aagtcgcaaa gatggcttat tatacagagt taatgagtcc tgataatatt atggataagg
     7921 ttcttatgaa taagcatttt tcaagaaaac atggaaaaaa tgcatactat ggtcaaaaat
     7981 gattatctgg aggtgaatta tgctagactc tttaaaaggt aaagttgtgg aaatagcaat
     8041 taaagcagag cattatggtt tatgtaaaga aaagactggt aattttagta ttagagataa
     8101 ggatactgga tatatactta taaccccatc aggtgtagga agagaagaat taaaagttga
     8161 acatatttgt gtattggatt tggatggaaa tgtaatagaa gttgagaaag gtattaagcc
     8221 aagtagtgag gtattgatgc atattgaaat atacaaaaca agagaaaata taaatgccat
     8281 atcacatact cattctttat atgcaacagc attttcagta gcaaagaaac caattatacc
     8341 aattgtatat gaatcagtta attatggcgg ttatgtagat attgcttcat acgaaagacc
     8401 aggaacttta aaattagcta agagtgtttc aaaattactg atagatacag atgcatgctt
     8461 attagaaaga catgggttga caactgtagg taatgattta gacgaagcat tattaaaatc
     8521 aagatatgtg gaagaggtag cagaaatata ctataggtct ttagtattaa atcaatttaa
     8581 agaacttgaa gtagtagatg ttgatgagtt taaatcttgg aaatacccag aagaaataaa
     8641 gttttaatct aaataatttt cattgaagtg gttaattaaa atatatgtat tatttatatc
     8701 aattactatg ttttattatg ttaattaccg tattttgcaa atatatttat tacatttttt
     8761 caaaaaaata tagaatccga aagccatata aaattgactt gggattctat actttttatt
     8821 tatgcatttt tctttatctg attttctgga gaatttatat gttcaaaata ttttttagtt
     8881 tcagatacta ttataggtga taatgcaagt aaagctataa ggttaggtat tgccataagc
     8941 ccatttacaa tatcagcaat tatccaaaca acttctaact ttaagaaagc acctaataac
     9001 accatagcca caaatacagt tctataccca ttcatgcctt ttacaccaaa cagaaattca
     9061 aaacatcttt ctccataata actccatccc aaaattgtag taaatgcaaa taatgttaaa
     9121 ctaactgtaa gtaatattgg tccaaatgta ggaattgctc cgttaaaagc tgcttgtgtc
     9181 ataacagctc catttaggtc tgaacaccac actcctgaaa taacaagaga gaatccagta
     9241 agagtacaga ttataatagt atctataaaa gtacctgtca tagatataag accttgttcc
     9301 gctggccatt cagttttagc agcagctgca gctattggag cactacctag accagactca
     9361 tttgaaaata caccacgagc gataccattt ctaattgcca gtgacatagt tgctcctaaa
     9421 aaaccaccag tagcagcagt aggtgtaaat gcacttttaa ttattaacat aaaagtttca
     9481 gggattttat tccaaaaaga tattaaaatg attccacaga ttacaacata tataactgcc
     9541 ataaaaggta ctattttagt agctacatta gatatacttt taagtcctcc aaatacaact
     9601 aaagctacaa atactgctaa tacaacccct gtaattctag ggtctatccc gaaactatta
     9661 tttattgcat ctgttattga atttacttga gtaaaagtac cgattcctaa aagtgcaact
     9721 agtataccac ttatagcgaa aaatattgca agaggtctcc atttttctcc cataccattt
     9781 actatgtaat acattggccc accagacact tgaccatttt tatctttagt tctgtatttt
     9841 attgcaagaa caccttctga atacttagta gccattccaa agaatgctgc tatccacatc
     9901 caaaaaagtg caccaggtcc accagctttg atagctgtag caacaccaac aatattacca
     9961 gtaccaactg tagcagctag agcagtacat aaggctgcaa aacttgaaac atcaccacta
    10021 cctttgtttt cagcagagaa tattaactta agagctcttg gcagtttagt tatttgaaca
    10081 actccaagct taacagtaag tagtatacca gtaccaacta atagtaccag taggggtggt
    10141 ccccaaataa aagcatcaac tttgtttaaa atatcaatta gattcataaa aacactccct
    10201 catttttttt gctttacata atcaatagaa tttttaatga agtaattgac aggaaataag
    10261 tataatttat taataatttc tagtgtaata aaaaaagcta aatctaaaaa agattcagct
    10321 ctggatgtac actaattagc aaccaaaata aaagtattag taaatactgt ttttggtaga
    10381 taatccctgt ccttttgcct gagagattga gcataaaagc cttgcccctt cggcgcccat
    10441 aatgggtctc tccagagttc cgtaaatttt acagtcctac ttttaaaagt acctgagagt
    10501 gttactcctt cggtgtgcta tatagcaact cttctataaa atcttaaccg gatattgatt
    10561 tgtcatagct tattgtatta ataatattaa tataagtttt tatgttttgt caactaaaaa
    10621 attaaaaaac aaagaaaaaa tataaaaatg taagcctatt tacgaattat aaacttagaa
    10681 agatgagtaa tatacgtgaa aatgaatttt gtaatacaca tacaaatgaa gtgtaaaata
    10741 agttttaaat aaaaaaataa taaaatctta aataattaat tagatatgat taaattatgg
    10801 taatattata gttacgatat taagaaaagt tctatttagt tagattttaa gcgtaatatg
    10861 agattatgta tagagaaagg atgtattttt agatgggaaa aatattagtg ctagcagaaa
    10921 aaccttctgt tggaagagat atagcaaaag ttttagggtc aaaaaatgaa aaaaatggtt
    10981 atatagaggg gccaaagtat gtgataactt gggcactagg tcatttggtt acacttgctg
    11041 accccgaaag ttatggagaa agatataaat catggagctt agaagacctg ccaatacttc
    11101 ccaagcacct taagactgtt gtaataaaaa agagtggaaa gcagtttaat acagttaaat
    11161 cgcaaatgaa tagaaatgat atagatgaaa tagttatagc aacagatgca ggtcgtgaag
    11221 gagaacttgt agcaaggtgg ataatagaaa aatcacaggt aaaaaaacct ataaaacgtc
    11281 tttggatttc ttcaagtaca gacaaagcaa taaaagacgg atttgcaaaa ttaagaagtg
    11341 gaaaagaata tgaaaatttg tattactcag caatagcacg agcagaagct gattggatta
    11401 ttggaataaa tgcaaccaga gctttgacaa caaagtacaa tgcacagctt tcttgtggaa
    11461 gagtacaaac tcctactctt gcaatgttgc ttaaaagaga agaagaaata agaaatttca
    11521 aacctaaaga gtattatgga ttagaattaa ttgctacaaa gggaaattca gatataaaat
    11581 ttatttggaa tgataaaaac aataattctt caacttttag taaagaaaag atagaatcca
    11641 cattaaaaaa agtaaaaggt gtagacataa aaatatctga agtaaacaag tcatataaga
    11701 aaaaatattc acctgcactc tatgatttaa cagaattaca aagagatgca aataaaatgt
    11761 ttggattttc agcaaaagaa actttatcta taatgcaaaa actgtatgaa catcataaag
    11821 tacttactta tcctagaact gattctagat atttaacatc agatattgtt ggaacattaa
    11881 aagatagaat aaaagctgta aatattagtg attattcaaa gatttgtaat agattattaa
    11941 aaagcaatat tatagctaat aaatcgtttg ttgacaattc aaaggtaaca gaccaccatg
    12001 caataatacc aacagaacag aaggtattca tgagtgattt tagtgataaa gagcgaaaaa
    12061 tatataatct tgtagtaaaa agatttttag cagtactatc tcctcctttt gaatatgagc
    12121 agacaactat aagtgcaact gtagaaggag aaatatttaa ggctaaggga aacaaagtaa
    12181 taaatttagg ttggaaagaa agttataaag atattgaaga tgaagatgag tatgaaagta
    12241 taccaaatgt tttagaaaaa gatgtactta aggtcaaggg attaaagata acttcaggaa
    12301 aaacaaatcc accaccatac ttaaatgagg gaacattatt gactgctatg gaaaagaatg
    12361 gtctagggac agttgctaca agagcagata taatagaaaa attattcaat tcatttttaa
    12421 tggaaaagaa aaataaagat atatatataa catcaaaagg aagacaatta ttagatttag
    12481 ttccagtaga cttaaagaca ccagaactta cagcttcatg ggaaaagaaa ttattggata
    12541 tttcaaaggg aaaacttagt aaaaatacat tcataaatga catgaaagat tattcaaaag
    12601 atattgtact agaagtaaaa aatagtgata ataaatttaa acatgataac ttaactaaaa
    12661 atagatgtcc agaatgtggt aaatttatgt tagaggtaaa tggtaaaaaa ggtaagatgt
    12721 tgatttgtga agatagagag tgtaatacta gaaaaactat atcacaagta accaattcaa
    12781 gatgtcctaa ctgccataaa aaattagaac ttcgtggaga aggtgagggg agactcttca
    12841 cttgtagttg cggatacaga gaaaagttgt cttctttcaa caagagaaag caagaagaaa
    12901 aacagaaggc atcaaaaaaa gatgttaatc aatatttaaa aaatcaaaat aaaaatcaag
    12961 agacatttaa taatccattt gcagaagcat tagctaagct aaaaaacaaa taattttaaa
    13021 gttattatta tacacaatta taactttaac tgtatattag ttatatgtac tgatataata
    13081 tagtgtatag tttaggcatg aaagtagggc tgttaaatgg atttaagtgg catatttaaa
    13141 tactattgta aagagtgtga aaatacatgg aataattcga gtgttgaatt atttgagaat
    13201 atagaaacgt atagtaaaga ttcacaaaaa aagagggaaa aagaattaga taaattgcta
    13261 aatacaatat cagttcattt agagaggtat ccaagtgatg ctgtattgag aaaaatgtgg
    13321 gtaaaaaagg gcgaggtttt cttacaaaag acattggaaa aagaaaatat ttttaagtta
    13381 gaaaaaatgg atgtagagga tagaaaaaaa tttttagata taacaaaaca gtttattaga
    13441 gatgctagaa aatttgatga tgatttacct ataggtgata ttatgcaagc tatgaggaat
    13501 gtatggattt caaatgcatt acaattatta tttggtaaag aagtatatta ttcaaaagct
    13561 aactttgcat atagtatgtt atatccatat actgataatt atttagacaa tacaaatata
    13621 gataaaaatg ataagatttt atttaataac tggttagaaa aaaggctcct gggagaacac
    13681 attaaatcta aggattatca tgaaagtaaa gtatctcaaa tgatagatta tattgaaagt
    13741 gtatacccta gagaaaagtt tacagaagtt tatgaatcgt tattattaat atttaaaagt
    13801 caagtaaata gtttaaaaca acatggtaag gaaaatcatt tgtgtaaaga agatttatta
    13861 tccatttcta ttgaaaaagg aggttcatcc gttttagtag atggatattt aataagtgga
    13921 ttgatgacaa aggaagaaat agagttttgt ataggatatg gatttttatt acaaatatct
    13981 gatgatttac aagatataaa agaagattta aaatacaacc ataaaactat tattacagag
    14041 atgtcaaaag agggtacttt agataaagtt gtaaataaac taataaattt tactattgag
    14101 ttaatagata gttttaaaat taataataaa aataaatctg taatagctat gataaagaat
    14161 gattgcttaa tgttaatttt attttctgta gtttataatg ctgaattttt ttctgtagga
    14221 tatataaaag aagtagagaa atttattcca tatacaatag attattcatt agagattgaa
    14281 gaaaaaataa aagaaaaatt taaaaatata gatgttttaa ataatgaaaa tgaatataaa
    14341 gaaatgattg atattatttg tgcagagtag aaaaaaaggg agtgtcttag aatagacttt
    14401 ttagtttatg aggcactctt tttgtataaa atcaaatgta ttttcatcat tttaacaatt
    14461 aaaaagagct tatccctatt tgagaaagcc ctttttaatt taaataaaaa tgtcaaggtt
    14521 aaactattta aatgcagtaa taatcccatt atcatgtaag ttttttatga ttgtagctgc
    14581 gatagggaat ccaaataatc cagctattcc aaatagttga gctccaacaa acatacacat
    14641 taatgttatt aaaggatata ggccaatttg gtgtcctact acctttggtt ctaatatttg
    14701 tctaacaacg gttataatta tgtatagtat taaaagtcct attgctaaat taatatttcc
    14761 attaatgagt gagataatca tccaaggtgt caaaatactt cctgtaccca agactggcaa
    14821 aatatcaaca agtgctatga tgacagatat agtaaatgca ttttcaacct ttaggatagt
    14881 aagtccaata agtagttcta tgaatgtcac tgatagtagt attgcatagg cttttagtag
    14941 ttttaaaaga gcattaatac tgctattttt tatatttatt atcataaatt gacttttttc
    15001 tggaaattgt tttaagataa agtttactat tttttgatag tcaaaagtaa agaaaaaaga
    15061 agcaattaaa gtaataataa gttttaacat aaatgcaggt gttttacttg caatactagc
    15121 aatagcattg agtgcatttg ttgaaataga agctactaaa gacatcatag attgacttat
    15181 attatcccaa ccaagtatta atgctaggtt aacattaggt gtactagcat ctatctttga
    15241 aaataaagta tttagcgttg gttgtatact tgatttatat atttcaggaa gtctaaaaaa
    15301 tacatcactt acacttgcaa atatcttcac accaaagcta actaataaaa atatacacaa
    15361 cccataaaaa attaaaagta ttactataga aataagtgta cgatttaaat ttgtattatt
    15421 tgtaataagt cttataatag gtcttaagat ggatgcaatg ataaaactga ttacaaaagg
    15481 cattaataat ggcaataaga attttaatac aatatataca atacttgtta ttaaaactac
    15541 atatgtaaat ttaattataa aattcttttg cttttgaata tccataatat aaaacctcca
    15601 atgtatttga tattattcta aatatggtct tttttacaga aaatatacta ctgtatattg
    15661 attataaact ataaagctta tgtaaagtaa ataaattatg taaaatttag gttaattttg
    15721 tataaagaga tacttttata ttttacaatt aataaccaaa tactaaaaaa tattttaaat
    15781 aaagaactta aaattgtgat aaaattaatt tatagatatt aaaaaaagta taatatatgg
    15841 tttttaagag gtgctaagat gaaaaatttt agagatttag gtgggaacaa aactgaggat
    15901 ggcagaactg taaagaaggg attattttat aggtctgcaa aattatcaaa tctaagtgaa
    15961 aatgatataa agattttaaa agagttaaat attaaatata tatttgatta tagaagtgat
    16021 gaagaagctc gtaaacatcc atcaacaata atatcaaata taaaaaatat aagaatacca
    16081 gcaatgagag agctagaaga atcaggagga agttttggtt ctattgaaga tatgattgat
    16141 ggattgtttg aaaaagatgg tgcatttaat atgttaaata atagttacta taatttgcct
    16201 ataaataatc cgtcatataa aaaattagta gaacttataa gagactattc taatttacca
    16261 atattaaatc attgtacagc tggtaaggat agaactggtg tggggagtgc tataattctt
    16321 atgattttag gggtatcgag agagaatata atgaaagatt atctaaaaag taatgatttt
    16381 gcagataaag agatagaaag atttatagaa tataagccta aatttaagga cattccaaaa
    16441 gaaaatttga aatatatatt tggtgtaaat gaagagtata tgaaaactgc atttagaaga
    16501 attgatgaag aatacattag tgtagaagca tatttatatg gagaatttaa tttaaataaa
    16561 gaagaaataa gaaaattaag aaatcaatat ctagagtaaa aggtgagttt atgagtttaa
    16621 atataacagc aagacagata aatataataa aaatattgtt aaattcaaaa gatacaatta
    16681 gtggtattgc cttaagtcaa gagataggat gttcttcaaa aactatacaa aatgaaataa
    16741 aggatattaa taaacaactt agaaatggta aaattttgtc aataagagga gttggttata
    16801 aactagaagg aagttttgat gaggtaaatc taaatagcaa tctctatgat gatgtagata
    16861 gagtagaata tataatcaga aaaatattaa ccttaagtaa tgaagaaaaa aatactataa
    16921 aacttgaaga tttagccgat tctatgtatg taagtgtttc aacagtaaaa aatgatttaa
    16981 aagaagtaaa aagaatgttg gaaaagtatt caatatcagt taaatctaag cataaacaag
    17041 gtatatgtgt tttggaaagt gaagataaga tattaaattg tatagttgac ttaagcaata
    17101 agagagataa tcaattgagt ttaaatgatt ttttaaatga tgatataaag gaaagaaaac
    17161 tattaataaa aaaattatta ttagacatat taaatgaaaa tggattagta ttaactggta
    17221 tagaatttaa gagtgtgtta aataatatat tagttatgtt aagcagacat aagtataatg
    17281 aaaaagaatt tatagagaat tatatagaaa attataggag caaaagaagc tcaattttag
    17341 aaaatgaaca aaacaaagaa attataataa attcaataaa aacattttgt aaaaatttaa
    17401 aattagctac atcaatagat ataagtaatg ataaggtatt tgaagagttt ttgtataagc
    17461 atattagtag tttatgtaaa aaaataaagt taggtataaa tcaatcacct ataatggctc
    17521 aagatataaa aataaaatat ccatttgctt ttgaactagc taatatagct aaaaagacaa
    17581 tagaaaaaga tttgaagata gaaattaatg aagatgaagt tgcaaacata gctctacata
    17641 taggaggagc agttgaaaga gcatcttata ataataaaga taaggttttc aaaactataa
    17701 tagtttgtac ttcaggcata ggcacatcaa tgttaataaa agctaaatta gagaatatat
    17761 ttaaggaaaa attagaaata ctaaagatta ttccttcata cttagttgaa tatataaaag
    17821 ttatagatgt tgattttgta atatctactg ttcctattga agtaggagat gtgccagtaa
    17881 taaatatatc tccaatttta agtgagaaag aaattagatt aattgaaaaa tatatagaaa
    17941 caggaaatat atacctagat ttagatataa aaagcctgtt tgatagtgaa ttgtttttta
    18001 ctgatttggt gtttaaaagg aaagaagaag ttatagattt tatggcaagt aaacttgtac
    18061 aaaagggcta tatagatgag gatatgaaaa aatcttactt tgagagagaa aaaatagcaa
    18121 caacagaaat agggaatatg gtaactatac cacatggagc tattggaaaa atttttgata
    18181 ataaaatagc aattggtata ttaaagaagc ctatttcttg ggaaatatca gatgttcgtt
    18241 taataataat gttagccctt gataaagata aaatattaga ttatgaagta atattttcaa
    18301 aaatatataa aagagtagat tctatagcaa aagttataag tatatgtgaa aatagtagtt
    18361 ttgaaaaatt tgtaaattta ttcaattaga tgggtagtat tgtatataca cctgtaaatt
    18421 tggataatat aaaactaaat aagataaaaa cgataaattt ctcttttaat taagagaaat
    18481 tttttgactt tcaaggatat taatataaag ttaagatata aacataaagc aaataaataa
    18541 aaaagaggtg gcaatatgat ttatacagtg acattaaatc catcaataga ttatatagtt
    18601 aagctagatg agctaaagac aggaagtaca aatcgtgtaa aagaagaata cgtttatcca
    18661 ggaggaaaag gtataaatgt atctcgcata tttaaggaac ttggaaatga taatacatct
    18721 ttaggtttta taagtggttt tactggagaa tatataataa gaacattgga agaaaaagaa
    18781 ttgaagacag attttataaa gataaaaagt ggtttttcaa gaataaatgt aaaaataaag
    18841 gcgttaaagg aaactgagat taatggacaa ggtccaaata tagatgatga agacattgat
    18901 actttatata aaaaacttga taagctaaat caagatgata ttttaatatt ggcaggaagt
    18961 ataccatcta ctttagatga aaaactatac gaaaatataa tggctagatt agaaaaaaag
    19021 aatataaaag ttgtagtaga tgcaactaaa aatcttcttc ttaatgtttt aaaatataaa
    19081 ccttttctaa ttaagcctaa taacgacgaa ttagaagaac tatttggtgt taaattaaat
    19141 tcaattgaag acatggtaaa atatgctaga aggttaaagg aaatgggagc tataaatgtc
    19201 ttggtatcaa tgggaaaaga tggagcattg ttgattacag aggaagatga agtgcttata
    19261 agtgatgttc caaaaggaaa agtaaaaaat tctgtaggag caggtgattc aatggtagca
    19321 ggatttatat ctgggtattt gaatactggg aaatatgatt atgctcttaa attgggagca
    19381 gcttcaggta gtgcaactgc tttttcatat gacttagcca aaagagaata cattgataaa
    19441 ttggtaaatg agatatctgt aaagcagttt taaaatatta aaaaatatat agaaaggtaa
    19501 ttaatattta tgaaaattat agatttatta gatgaaaagt caattaagtt aaatttaaat
    19561 tcaaaaacca aatcagaagc aatagaagaa ttagtagatt tggtcgcaaa tagtggaaat
    19621 ttaaatgata aagaaaacta caaaaaagca attttggctc gtgaagaaat gagtacaaca
    19681 ggaattggag aaggtgtagc tatacctcat gctaaaaatt cttcagttac taaagcttgt
    19741 atagcagcag cagtgtcaaa agaaggtata gattatgaat cgtttgacgg aagtttatca
    19801 aatctatttt ttatgatagc tgcaccagac ggagctaaca atactcatct tgaagtatta
    19861 tcaagattat ctacaatact tatggatgaa gactttagaa ataaacttat aaattcaagc
    19921 tctgaaaaag aatttaagga aataatagat aaaaaggaaa gagaaaaatt cagtgaagaa
    19981 tatcaagatg agaaagttac tgaaaaaaat ataatagata ctaaagagaa tgatgctaat
    20041 aagtatccta aggttttagc agtaacagca tgtccaactg gaatagcaca tacatttatg
    20101 gcagctgaaa gtttaaataa aatggcagaa aacaaaggtg tatcaataaa agtagaaact
    20161 aatggttcag caggtgttaa aaataagctt acaaaagatg aaatagaaaa tgcaacatgt
    20221 ataatagtgg cggcagataa aaatgttgag atggctagat ttaatggtaa aaaagtcata
    20281 aaaactaagg tggctgatgg tatacacaaa gcggaagaac ttatagacaa ggctgtaaat
    20341 ggagatgctc caatttacca tggaggagat ggaagtcaca acgaatcaaa tgaagaaagt
    20401 gaaagtggat ttagaaaagt atacaaacac ttaatgaatg gtgtatctaa tatgttacca
    20461 tttgttattg gtggagggat acttatagcg atagcctttt tacttgatga ttatactatt
    20521 aacccttcaa actttggttc aaatacacct atagcagcat tttttaaagg aataggagat
    20581 aaagcttttg gatttatgtt acctgtactt gctggatata tagcatactc tatatctgat
    20641 agaccagcgt ttgttgtagg atttgtagga ggtgctctag caggtgatgg tggttcaggt
    20701 tttttaggtg cactacttgc aggttttata gcaggttatt tagtagaagg gcttaagaaa
    20761 atatttagtg tactaccagc ttcattggaa ggaataaaac cagtattgtt atacccttta
    20821 cttggaacac ttttgatggg aattataatg acatttttag taatacctcc agttacagca
    20881 attaataatg caatggttgg atttttaaat ggtttaggtg gaacttcaaa aatagtttta
    20941 ggattagtat taggtggaat gatggctgtg gatatgggtg gacctgtaaa taaagcagca
    21001 tatgtatttg gagttgcatc acttgaatca gggcagtttg aaataatggc agcagtaatg
    21061 gctggtggta tggttccgcc acttgctata gctcttgcaa ctacattttt taagaataga
    21121 tttactaaag aagaaagaga ttctggtaaa gttaattatg taatgggatt atcatttgtt
    21181 actgaaggag ctataccttt tgcagctgga gacccacttc atgttatacc agcttgtgtt
    21241 ggaggttcag cagtggcagg agcacttagt atgttgttca acgcagctct tcgtgcacct
    21301 catggaggag tatttgttat accagtggtt acccatccat ttgcatatat attagctata
    21361 gctgtaggtg ctttggttgg aatgatgctt ttagcattac ttaaaaaacc attaaatcaa
    21421 gaagtatagc aattaaagga tattcaagaa cttaaaatat gatgttttat aaaaattaat
    21481 atattgctga attattttaa aataaggatt ttatgaggct gttatataac gatttgtaat
    21541 tgttatcttg acagtctttt atttttatat aagtaaaata agtgtgtttt gctttatttg
    21601 tatttaaatt gtttttaagt tagaatataa agaaaattat atattatttt aatattcact
    21661 cgaactagaa aataatattt gttgtatttt tttgtatata aaatatattg taatattaaa
    21721 aaaataatag taaactgatt gcataaaaat gatttttata taaaatttaa gcagaagaat
    21781 aaatttctaa cgaaattgga tatttcttct gtaaatacat attcaaaaaa agaattataa
    21841 gagtttagtc agcagtcgtg taagtaataa ggactttata aatatataga aatataatga
    21901 tattaatata accaagcgat ttttaattta gctttttaat aagcttattt tttatactat
    21961 ttataataaa taaactccat gatgggaaaa ctattactat ttaatatgta aaggagtatt
    22021 ttaaatgaat gaatcatatt ggtttttaaa ctcaagtcca aaagaatata acaaattagg
    22081 tgaaaatata aaaacagatt gtcttatagt aggtggtgga ataacgggat tgacaactgc
    22141 atatttatta gcaaaagaag gtaaaaaagt agtgcttgtt gaggctgata aaataggata
    22201 tggaacttct ggaaggaata ctggtaaggt aacttgtcag catgatatat tttactctaa
    22261 gatagagaaa aaatatggat tagataaagc aaaatcatat tataatgcaa ataatgaagc
    22321 tttaaattta gttgaacaaa taatagaaga aaacaatata aaatgtgatt ttaaaaggga
    22381 aacttctttt gtatttacag aaaaagagga tactataaag gatattaaag atgaatatcg
    22441 tacatgtaaa aaaataggaa taaattgtga gtatcatgaa actatagaaa atattccatt
    22501 agatataaag ggggctatta gttttactaa tcaaggtcaa tttaacccaa agaagtatat
    22561 tgatggattg gcaaaagcag cagtaaattt agggctaaaa atatatgaaa atacaccagt
    22621 agtagacctt gaaaaaggaa aaatatgtag ggttaaaaca agagaggata atataataga
    22681 agctgagaat gttataattt catctcatag tccatggtat gatggattga atttttattt
    22741 tgcaaaagaa tatgcagagc gtgcttattt aatggttgca gttttggaaa ataaattagc
    22801 agatggtatg tttataagta tagatgaccc atcaataact tttagacaat ataatgatgg
    22861 aagtgaaaat ttactgattt ttggaggagg agaccataaa gttggtcagg gaggaactga
    22921 gaaagaaata tttgatgatt tagagcatta tggtaaagaa gtctttaagg taaaagattt
    22981 taaggggaaa tggtctgctc aagataatat gagttttgac aatgttcctt atattggtta
    23041 tatcaacaaa agagaagaca atatttatgt agctacaggg ttctctaaat ggggaattac
    23101 taatggaact gctgctggaa taataataaa agatttaata ataaataata attctgatta
    23161 taaagatact tttaatccat ccagactagg tagctacttt tcaaaagatt ttataaagga
    23221 aaatgcaaac gtagcaataa attatgtaag tggaaaatta aaaataggta gtggagatat
    23281 gcctaaaaat aatggagaag gaaagattgt taatattgat ggaaaaagat atggtgtata
    23341 taaggatgat aatggagatt tttatattgt agacactaca tgtactcatt taggatgtga
    23401 acttaatttt aacagtgaag aaaaaacatg ggactgtcca tgtcatggtt ctagatttga
    23461 ctataaggga aatatacttg aaggaccagc tcttaagcca ttaaaacttt atggacatgg
    23521 tgataatgat gttaatccta agttattata aaattatttt taggaaggta aagtgtatat
    23581 gaataaactt ttaaagccaa taactatact attgtatata gtttatattt tatctgccgt
    23641 attttttgta gcaaacaaag aatatggaag tctaggtcta gtagcaatta gtttagttgg
    23701 tactttcatt ttgagtttta taaataagca aactgaaaga ttattgagta aagaattata
    23761 tataatgctt gctttattta taatgttttc atcattgtta ggtacttgtt ttaattttta
    23821 tgatataaat tattatgatg attttttgca tttatggtca ggtataatat cttgtattgt
    23881 ggctttttct ataattaaat acttttatac acaaagagag ataagtaaaa tgagtaaagt
    23941 gtttttagtt atatttttat ttatgtttag tatgggagta gcgagtttat gggaaatagg
    24001 tgaattttta atggatactt ttgttgggac tactacacag gccggagggt tagaagatac
    24061 tatgattgat atggtagatg ctttaatagg cacaattata actattcc
//
LOCUS       DAYAOX010000042        23359 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.042, whole genome shotgun sequence.
ACCESSION   DAYAOX010000042 DAYAOX010000000
VERSION     DAYAOX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.042"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            206..1837
                     /locus_tag="ACGI57_001306"
     CDS             206..1837
                     /locus_tag="ACGI57_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9716390.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(1972..2898)
                     /gene="fba"
                     /locus_tag="ACGI57_001307"
     CDS             complement(1972..2898)
                     /gene="fba"
                     /locus_tag="ACGI57_001307"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HFG9716391.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            3228..3557
                     /locus_tag="ACGI57_001308"
     CDS             3228..3557
                     /locus_tag="ACGI57_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HFG9716392.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            3557..4279
                     /locus_tag="ACGI57_001309"
     CDS             3557..4279
                     /locus_tag="ACGI57_001309"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFG9716393.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            4306..5667
                     /gene="rlmD"
                     /locus_tag="ACGI57_001310"
     CDS             4306..5667
                     /gene="rlmD"
                     /locus_tag="ACGI57_001310"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFG9716394.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVLL
                     RRK"
     gene            6059..8128
                     /locus_tag="ACGI57_001311"
     CDS             6059..8128
                     /locus_tag="ACGI57_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9716395.1"
                     /translation="MQLTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSAL
                     MKLFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFF
                     KNMVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYE
                     LDQIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEE
                     KGVESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVE
                     ELGDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHIS
                     PHLLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSII
                     LPNEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEI
                     SIVPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNV
                     LLYTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKD
                     KYISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGS
                     TYTEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMID
                     DSGGGRK"
     gene            8125..9990
                     /locus_tag="ACGI57_001312"
     CDS             8125..9990
                     /locus_tag="ACGI57_001312"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFG9716396.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            10114..10308
                     /locus_tag="ACGI57_001313"
     CDS             10114..10308
                     /locus_tag="ACGI57_001313"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716397.1"
                     /translation="MKKKTKISKKIVSFIMVFITIVTTILTSSVSAYASELDMSEQTG
                     FNYLGISPITGKKLIITFIV"
     gene            10311..10928
                     /locus_tag="ACGI57_001314"
     CDS             10311..10928
                     /locus_tag="ACGI57_001314"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716398.1"
                     /translation="MDNKIVFCVEPGIKTSSGGGYISEELGDKFISTTLPNYHKRKAE
                     ILEKVNRHNILPSWNNEKVAVKSGETIELEDKNGIIREMQLESNNTGTDILLEENTLK
                     ITANKNSNSGVISYIKIPKNEVGISIVYRKHNYQSLSEFHMESSMSANLNINVIKLGI
                     VKVKKIDEGTGKELANAKLRFEYDGDSKEIITGSNEIASIEDIIG"
     gene            10920..11441
                     /locus_tag="ACGI57_001315"
                     /pseudo
     CDS             10920..11441
                     /locus_tag="ACGI57_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901831.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            11554..11715
                     /locus_tag="ACGI57_001316"
     CDS             11554..11715
                     /locus_tag="ACGI57_001316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716399.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            11955..12182
                     /locus_tag="ACGI57_001317"
     CDS             11955..12182
                     /locus_tag="ACGI57_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9716400.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            12179..15118
                     /locus_tag="ACGI57_001318"
     CDS             12179..15118
                     /locus_tag="ACGI57_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFG9716401.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVHGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            15118..15792
                     /locus_tag="ACGI57_001319"
     CDS             15118..15792
                     /locus_tag="ACGI57_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716402.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            15953..16369
                     /locus_tag="ACGI57_001320"
                     /pseudo
     CDS             15953..16369
                     /locus_tag="ACGI57_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            16329..18776
                     /locus_tag="ACGI57_001321"
                     /pseudo
     CDS             16329..18776
                     /locus_tag="ACGI57_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            18916..19338
                     /locus_tag="ACGI57_001322"
     CDS             18916..19338
                     /locus_tag="ACGI57_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716403.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            19876..20910
                     /locus_tag="ACGI57_001323"
     CDS             19876..20910
                     /locus_tag="ACGI57_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HFG9716404.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            20997..21200
                     /locus_tag="ACGI57_001324"
     CDS             20997..21200
                     /locus_tag="ACGI57_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFG9716405.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            21371..22531
                     /locus_tag="ACGI57_001325"
     CDS             21371..22531
                     /locus_tag="ACGI57_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HFG9716406.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            22790..22930
                     /locus_tag="ACGI57_001326"
     CDS             22790..22930
                     /locus_tag="ACGI57_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716407.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT         9433 a         2337 c         4118 g         7471 t
ORIGIN      
        1 ataaaataca attaaaagca aaatgaaatt tttaacaaac tataatcatt aaaattatat
       61 cttagttacg ttttaatgaa ataaactact tgaaaagacg aatattataa ggagttgatt
      121 atatgtgctg atggtattta ttgtaagcaa acaataaatt ttgtatgtag ttcaataaat
      181 tacttaatga aaggtgcgaa tattaatgga taataattta atagggctta tagaacatgt
      241 agaaaatcca gccattttat gtaaagaatc aggcgaaata atatactgta atcatctaat
      301 agatagtatt tttagttttc tagatataaa aaagcctaga aatataaatg aattagattc
      361 aaattttgac aaaacagaaa tactgacaga tagtaaaaag aaaatagctt tcagagagtt
      421 aaggatgact gcacatatat acaatatgaa agataataat aatgaaaata atatagttta
      481 tttatttgag aagtctctaa tatctgataa agtaattgaa gatataatag agcatataga
      541 tgaagttgtt gttgtgttta acaaagatgg ggtaattgaa aagatgaaca ctgtcagtga
      601 tgagatatta ccttttaaaa gaactgaggt acttggtaga aatataacag accttgtgag
      661 acaaggatta gttgaagaac caataatatt aaacatgctt aaagtgaaga aaaagattta
      721 tagaaatatc gtttacccag atgggaagct tattgcatat acggcagttc caagatggga
      781 ttctaaggga aagcttacag gaggagtact aacagggaga gatatttcaa gggttattaa
      841 gcttgaatct cagataaaat atagtgatat atcagaagac acagagtata taagtcaaag
      901 taagattatg gacaatataa aaaaagttgt taagagagct gcagcctcag attcttctat
      961 atttataaat ggagaatcag gtgtaggaaa agaaataata gcaagaacaa tatataagta
     1021 tagttcaagg agagacaaac cttttatagc tataaactgt ggagctatac caaatgaatt
     1081 attagagtca gagttttttg gatatgaaga aggttctttt actggagcta agaaaaaagg
     1141 taaaaaggga ctttttgaag aggcgaatgg tggaactatt ttcttggatg agataggtga
     1201 gttaccaatg cagatgcaaa aaaaattact tagggttata caagaaaata ctattactag
     1261 aataggtggt agtaagccta taaaaataga tgttagatat ataagtgcta ctaatatttc
     1321 tcatgaagac cttagaaata accttaaatt tagacaagat ttgtactata gattaagtgt
     1381 tattccagta aaaatacctc ctcttagaga aagaaaagag gatatagtac cattagtaaa
     1441 ttatttctta aaactttaca atgaaaagta caatagagaa gtagaagttt cgcctaaagt
     1501 tattgagtta ttagaagaat attcttggcc aggaaacata agagaattaa aaaacatcat
     1561 tgaaagattt gtagtattat cagctaaaaa tgtaattgga gaagatgaat ttaacatgct
     1621 gataaattta gatatgattg ataatgaaac agatgattta tcaccaatag tggtaaatgg
     1681 aattatgaat cttaatgatg catataaaat agtggaccaa ataatgataa gtaaggctat
     1741 aaataagtat ggttcaataa caaaggctgc tgaagttata ggtatatatc cttctaccat
     1801 ccatagaaag ataaagagtg gacatattca cgtttagctg tcagtttaaa aagacaatat
     1861 aattaaaata ttataagata cataatttat acaacttaaa agagatggct taaaaataaa
     1921 tttaattact ttaagtcatc tcttttcttt tataaatatt taaaagttat attagtttat
     1981 agagttagca gaacctaaaa catcatctat cttgctttca actacagctt gtatagcatc
     2041 tctaccagca ccaatgtatt ttcttgggtc aaattctttt ggattttcag ctaagaattt
     2101 tctgatagca gcagtcatag ctagtcttaa gtcagtatcc atgtttattt tacaaacagc
     2161 catagaagat gcttttctta acatatctac aggtacacct ttagcaccag ctatatttcc
     2221 accaaattca ttacatgtag caacagcgtt ttggtcaaca gcagatgcac catgaagaac
     2281 tattgggaaa cctggtaatt tgctttggat ttcttctagt atatcgaatc ttaatttagc
     2341 ttctcctttg aatttgaaag caccatgaga agttccaata gctattgcta atgaatcaac
     2401 acctgttctt tcaacgaact caactgcctc agctggttga gtatacttgt gaacatcaga
     2461 agtaacatca tcttctgttc cagctagaac accaagttct gcttcaacaa caacaccttt
     2521 agagtgtgca tattcaacag cttctttagt tattctaaca ttttcttcaa agtcaaaatg
     2581 agaaccatct atcataactg aagagaaacc agcatcaata caagttttaa tagcatccat
     2641 atttggacca tggtctaagt gaagagctac atctacacct atttcatcag aagcagcctt
     2701 aaccatttca actaatgtat gaggtccagc atatttaaca gctgacatag aagcttgtat
     2761 cataacataa gaatttttag ctttagcagc ttttaaaaca ccttgtaatt gttctaagtc
     2821 acttatgtta aatgcaccta tagcaaatcc accttcgtaa gcttttttaa acatttcttt
     2881 tgtagtaatt aatgccattt taaactcctc ctattaactt ttaaaattta cccatcactt
     2941 atattataac gttaaatcaa aaaaagtata tagtaaattg taataactat aaattacaag
     3001 tataatgtta aatagaagac aataggatta aataaaataa taaagaatat aaacaattta
     3061 ggaaaactag ctatataatg ctaactataa gggtaggaac taccttatta gtttggagat
     3121 actgataaca atagttgcct tgaccaagaa gttaccactt caaacgttat cgaaaggaat
     3181 taagtggatg ataattcact aaaaatttaa aataaaggag ataaactatg ccacagataa
     3241 aaataagagg aattaatgaa aatgatatat gtaaaataag tgaaaaaatg ataaatgatt
     3301 tagttgaagc tgtaaaatgt ccaagagact actttgaaat agaatgtata aagtcagttg
     3361 caataagaga tggtaaaata gcagatgtat atccttttgt tgaagtagct tggtttgata
     3421 gaggacaaga agtacaagat atagtagcaa gaataataac tgatagtata agaaataatt
     3481 tagatgtaga aagtatagat ttagcattta cagtatttga aaaagaaaaa tattatgaga
     3541 atggagagca tttttaatgg ctaaaaagca acgtatagat aaaattcttt caaatttagg
     3601 ttatggaagt agaagtgaaa taaagaagta ttgtaaacaa gggtctgtag tagtaaatgg
     3661 aagtgaagtt tcaaatccag gaactcaagt agacactgaa aatgatgaga tattatttaa
     3721 tggagaagaa gttatatata gagaatatat atatttgatg atgaataaac ctgatggata
     3781 tatatcagca actacagata aatatgaccc aacagtatta gatttaattg atttatcata
     3841 cttagctttt gaaccatttc ctgttggaag gttagataaa gacacagagg gattattagt
     3901 tttaactaat gatggaaagc tttctcatag agttttatct cctaagaagc atgtgcctaa
     3961 gacttattat gcaaaaattg atggagtagt tactgaagaa gatgtagaag cttttttaga
     4021 aggtgtagtg cttgatgacg gatataaaac tatgccatca cagttaaata tattaaaaag
     4081 tgatgatgaa tcagaaatag agcttacaat acatgaaggt aagtttcacc aagtaaaaag
     4141 aatgtttgaa agcgtaggca aaaaagtagt atacttaaaa agattgtcta tgggcaattt
     4201 aaaattggat gaaagtttag agttaggtga gtatagagag ttaacagatg aagaagtaaa
     4261 attgattgaa gaaagataat aaagatacta tagtaaattg gaggaatgtc tgtgaagaaa
     4321 aaagatgtta tagaatttga agtagataaa atggaatttg gtggtacatc tttaagccaa
     4381 gttggagata gagttatcca tatgaagggt ggaataagtg gtcaaaaggt gagagctgga
     4441 gttaaaaaag ttagaagtaa gaaagctgaa gttaagatga tagaattatt agagaactca
     4501 ccacttgaaa ctgaagaaac ttgcaagcac tttagagagt gtggaggatg tacactattg
     4561 tctgtgccat atgaaaaaca attagaaata aaagaaaaac aagttatgga tttattttta
     4621 aagcaagatt tatttggatt tcagttttta gggatagaag gaagtccaga aaacaaatat
     4681 tatagaaata agatggaata tacttttgga gatgaagtaa aaaatggacc tttgacatta
     4741 ggtcttcata aaaaaggaaa gcatatagat atacaaactg ttgaggagtg tatgttggtt
     4801 gatgaagact tctcaaagat attagtttct agtgtagagt ttttcaatga aaaaaagctt
     4861 ccttattata gaacaatgaa tcacaaagga tatttgagac atcttgtagt tagaaaaggg
     4921 attcatacta atgagattat ggttaatata gtaacttctt ctcaagaaga ttttgatatg
     4981 tatgagttta aagatatgct tttagggatt gatttaaaag ctgagttagt aggtgtactt
     5041 catactatta atgatggttt agcagatgca gtccaatgtg atgagttaag agtattgtat
     5101 ggaagagatt atatacaaga agaaatttta ggtcttaagt ttaagatttc accattttcg
     5161 tttttccaaa ccaatactaa gggagcagag gttctttata gtattgctag ggattttatt
     5221 ggagattaca atgataaagt tgtatttgac ttgtatagtg gtactgggtc tattggacag
     5281 gttatggctg gtgctgctaa gaaagtgtat ggtattgaga tagttgagga agctgttgtt
     5341 gctgctaatg agaatgctaa gttgaatgga cttactaatt gtgagtttat tgctggggat
     5401 gtggctaagg ttgttaagga tttaaaggac aagcctgatt tgattattgt tgaccctcct
     5461 agaccaggca ttcataagga tgctattagg gatatttgtg gatttggtgc taaggagatt
     5521 gtttatattt cttgtaatcc taagtctttg gttgttgatt tggttgattt taaggggtat
     5581 ggttatgaga ttaagatggt taagtgtatg gatatgttcc caaatacgcc acattgtgag
     5641 actgtagtgt tgctacgtcg aaaataatgc tagaaatctt gagctttaga cagtttcata
     5701 gacatataat atttagatta gagggtaata aaattaggaa taaagaattg aaaaggtgtt
     5761 ttgatgtgtc tgtagttata tatatggagt gtgggaatac gaaataaaaa aagttttaat
     5821 tatagaaata gatattaaat ttataaattt aatatctatt ttttatgtga aaatatattt
     5881 aatctaaaga atgatatttc ataaaaaaaa ttaaatatat ttttaaaata tttacaatag
     5941 agttattttt tattcgtata ataatagaaa tattttagca taagatgtat aataaaggaa
     6001 aaaatacaga attaaaaaac ataagataga taagtggact taggggagag aaattattat
     6061 gcaattgaca aaaataattc ttagtaacta taggtgtttt ggagaagaac ctacaactat
     6121 tgaaattaat aatttaactg gatttatagg tcataatagc agtggtaaaa ctgctttatt
     6181 aagtgcactt atgaaacttt ttggagaaaa gtctagtgat agaattattg aaagatcaga
     6241 tttccatata cctgaaagta ttgatccaga tagtatttct aagaatgaat tatatattga
     6301 agcacaattt gagtttcctg aattggagag aggtaaagac agcatatcta taccagtatt
     6361 ttttaaaaat atggttgtta gtgaaccagg tgggaaacct tatttaagaa ttagacttga
     6421 agcaaattgg gagaaaagct caaatcctga aggaagtatt gattcaaatg tatactatat
     6481 aacatctaca acagaggaga taaatcctga agataagcgt aatatcaaaa gatatgagct
     6541 tgatcaaata aagatgattt atgtacctgc cattaggaga ccagatgaac agttaaaaaa
     6601 tgtctcagga acaataattt caagattatt aaatggtata agttggtcag aacaaacaaa
     6661 ggcaagaatt aaaacgaaag caagtgaaac ggaggaaatt ttctttgaag aaaaaggggt
     6721 tgaatcatta aagaaatcac tcgaaactca atggaatagt tatcataagg atattagata
     6781 ttctaatcca aagataaact tcaatagtac tgatttagag acaattttaa aaaaagttga
     6841 agtaactttt tcacctacag ggacacatcg agattatcgg gttgaagagc ttggagatgg
     6901 gttacgttct ttattttatc tttcacttgt aaatacattt ttagaaattg aagaagaaat
     6961 tctaaaatct attaatgatg gtaaagaaga agatatattt acattaatac cgcctgtatt
     7021 aacaattgtt gcggttgaag aaccagaaaa tcatatttca cctcatcttt taggaagggt
     7081 agttgaaaaa ttaaatttta tttctacaaa agaaaattca cagacaatat tgacatcaca
     7141 ttcaccagca atagtaaaaa gaattgaacc aacagacata aggtatttta ggatttgtaa
     7201 ggaatatgaa tgtacaaaag tgaagtcaat tatattacca aatgaagaaa aggaagcaga
     7261 acaatttaaa tttgtaaagg aatcagtatt agcatatcct gaactttatt ttgcaaaact
     7321 tgttgtattg ggcgaaggtg atagtgaaga gatcattatt aagcagatga ttgaattaaa
     7381 agcagaatct gtagattcat cagaaatttc aatagtacca ttaggtggta ggcatgttaa
     7441 tcatttttgg cgtttattaa atgatttaga aattccattt attactttgt tagatttaga
     7501 tagagaacga gatggaggtg gttggggacg tataaagtat gcaatacaac aattaataaa
     7561 tattggtcat gataagaatg tattacttta tacatgtgaa ggaatttcac aagataatga
     7621 atttaaagag atgcatactt gggatgtaac tgatactgaa aatatggcta aatggattga
     7681 aatgctaaaa ggttatgatg tttatttttc agctccgtta gatatagatt ttgcaatgtt
     7741 acaaagtttt aaagataaat atatttcagc attaacaagt gcggaaggtc ctaggataaa
     7801 agatcatgga aaaatacaag atattgtgat caatgaagat gctgaagatg aatcattgaa
     7861 agctgcttat aaagatagaa tagatagtga tattaaatca accttaaaac aagaaggtgg
     7921 tgatggatct acttatactg agggagagaa agaactaatg atatggtatt catatttctt
     7981 tttaggaaga ggtaaaccaa ctactcattt atctttattt tctaataata ctgatataga
     8041 ttttgaaagt tttccatact gtctaaagga gtttgtaaat agtattattg aaaagatgat
     8101 agatgattca gggggtggaa gaaaatgaag aggaattggg ttagtaaaga agattggagt
     8161 ccagcggctg gattcagact agaagttgaa gcgatgaata cagttattaa tgataagaat
     8221 acacttgttg tagctggtcc tggcgctgga aaaacagagt tattagctca gagagcctgc
     8281 tttttactag agactaatac ctgtagatat cctaaaaaaa tccttgctat tagttttaaa
     8341 aaggatgctg ctgaaaattt aaaagagcgt ttagaattga gatgtgggaa agaattagct
     8401 tcaagatttg aatcaaagac ctatgatgct tttgcaaaag aaatcgtaga tagatttaga
     8461 aatagtttag atgaagcata tagaccttta aataattaca atatagctga aaatcgtgat
     8521 gttagaagag catttgagat agcaggatta aatttcagag gaaatcaaac tgaatttaat
     8581 aaagtatatg gaaataaact atcgataaat aaattgcctt tacatacaga ttttgtagat
     8641 aagattttta tagatgcttg gaatgtacta cttaaaggca acaaagatac taattttgta
     8701 agttcactta cttttggtat gatatcacgt ttagccgaat atttgataag aaccaatagg
     8761 tatattaaga aagcaattga aatgacttat agtcatgtgt ttttagatga gtttcaagat
     8821 actaaatcta ttcaatatga tcttgttaaa gcttgttttt ttaaaacaga aacaataatt
     8881 actgctgtag gggatggcaa acaacgaatt atgttatggg ctggtgctag aaagtcaata
     8941 tttgaagatt ttcaaaatga ttttttaagt caaaaaagaa cattaattat gaatcatcgt
     9001 tcagcgccaa gacttgtaga gattcaaaaa gcaatgtata acagtttgaa tgaagaggca
     9061 attgatttaa aaactaatga aaagtggaat tatgaagatg gagaagtgta tttaagactt
     9121 tttgaggatt atataaaaga agctgatata cttagtgagg aaatagtcga attggtggaa
     9181 acaggtatag aaattaatga aatatgtata ttagtaaaac agagcgtaga tatttattca
     9241 aaagagatta taaataaact ttctgaacgt aatattaagg cgcgtaatga agcaatgtat
     9301 caggagatat taaaagaaga tataataaag acactggttt cgctttttag gttgtcgtta
     9361 agtgatagaa atcccgatga ttgggattat atttacgata ttctaggtga attatatgga
     9421 atagacgaag ggtataaccc taaaatatta aatgatttca ttaccaaagt agaattaatg
     9481 attaatgatc tcaaggtaaa tttaaatatt actgatgaaa aacaattttc agatttagtt
     9541 gattctaagg ttgaatatat ttcatatgaa aaatttgcat caaagtttca aaaatataaa
     9601 tctaaagatt attttgatga tttggtagat aaatttaaaa agctggtatg gattgaatat
     9661 aatgaaacta aagattggat taaagcgatt gataactttg aaggtaaaaa ttcaattcca
     9721 ataatgacaa ttcataaaag taaaggactt gaatatgata ctatcttttt tgttgggtta
     9781 gaagattcag cattttggaa ttttaaaaat caaccagatg aagatagatg tgcatttttt
     9841 gttgcattat caagagctaa aagaagaatt gattttacat ttagcttaga tagaccagca
     9901 ggattttcga attcacagaa gcattattgt attaatgaat tctacgaatt gtttgataat
     9961 actgagattg taaaaaagat agagtattga ttaagatgat aaaaacttta gtatattata
    10021 ttttaagata ttagttatat aagtagacat tgaatttatg tatttgatgt ctatttttta
    10081 tataaaaata tattaaagaa aggatgaatt tgcatgaaga aaaaaacaaa aatatctaaa
    10141 aagattgtaa gttttattat ggtttttata actatagtta ctactatact tacatcatca
    10201 gtttcagctt atgctagcga gttggatatg agtgaacaga caggctttaa ttatttaggt
    10261 attagtccaa ttacaggaaa aaaattaatc ataacattta tcgtatgaag atggataata
    10321 aaattgtatt ttgtgtagag ccaggaatta aaacttcaag tggaggagga tatatatcgg
    10381 aggagttagg agataaattt atatctacaa cacttccaaa ttatcataaa agaaaagctg
    10441 aaatacttga gaaggtaaat agacataata tattaccttc atggaataat gaaaaagttg
    10501 cagttaaaag tggagaaaca attgagttag aagataagaa tggtattatt agagaaatgc
    10561 aattagaatc aaataatact ggaacagata tattacttga agaaaatact ttgaaaatta
    10621 cagcaaataa aaattctaat tcaggagtta ttagttatat aaagattcct aaaaatgagg
    10681 ttggaatatc aatagtttat cgtaagcata actatcaaag cttatcagaa tttcatatgg
    10741 aaagttcgat gagtgcaaat ttaaatataa atgtaatcaa gttaggaatt gttaaagtaa
    10801 aaaagattga tgaaggcaca ggaaaggaat tagcaaatgc aaaacttaga tttgagtatg
    10861 atggtgatag taaagaaatt ataacgggtt ctaatgaaat tgctagtata gaagatatta
    10921 ttggttgatt aaaaagtgaa gtatactttt gtatctatga aaaagactat aagcaatata
    10981 taaaattaga tatacctatt gatgaagcaa aaataaaaaa tagatttgaa ataaggttaa
    11041 aaaatgaaag agcatatcat gcagcagttg atttgttagc aaatagagat gaagaaaaga
    11101 cagcattttc aattattaat cgttatatac gttttgttga taaagatgat gaaaaaatta
    11161 gaagtgaatg gaaaactaat gatcagtgga gttattttat tggtgaaggt agagcgaagt
    11221 taaaactaac aacaaatcca gaaccatata atattcaaaa aactttaaat tggttatcaa
    11281 gacaagttgc accaacgtta aaaacaatta aaaggttaga tagtttgaac aactcaacat
    11341 taattgaggg cttaataagg tatgcaagac taacagataa acataaaaaa ataattgaac
    11401 aaaattcagt aaatgttgaa gacattattt atgatgaata aaaaaatgta attagtattg
    11461 cagattttaa tataaaagaa gggagatata cgaatgaatt ttggtcaaaa tttatataat
    11521 tagtttttat caaatgcaca atcattggta ttaatggcta tagctataat tggtgtatat
    11581 ttaggattta agagagagtt ttcaaaactt ataggatttt tagtagttgc attaattgca
    11641 gtagggcttg tgtttaatac agcaggagtt aaagatatat tattggagct atttaataaa
    11701 ataataggtg tgtaaggatt aaaagagaat agaaaattat gagtatatga tgaagggttc
    11761 taataagttt tattaaaata tatattgaaa gacaagtaga gctatttcga gttattacat
    11821 gtaatgctaa tatatataac tgcattttgg aaattaaata cataattaga aatagcaaat
    11881 tttataggtg acttattaat atgtttactt attgtatact atttgatata tttaatatga
    11941 ggtgattctt gaaaatggac atagtgaaag tttttgctaa caatgtgaga aaatatagaa
    12001 ataaaaaagg tctatcacaa gaagcttttg ctgaaaaagc tggattgcat agaacttata
    12061 tttgtgctat tgaaagaaaa aagagaagta ttgctttaga taatgtgcag aaaattgctg
    12121 atgcgcttga gattgagact tacttactat ttatagaagg tgaggagaaa gaatgtctat
    12181 gaaaaaatta ccaatagaaa agcttataga aaatttaggt ttttcagggt caaagaatct
    12241 tgtgaatata gatatgctta caaaattaga tatatctaaa catgatgtaa aaactttaaa
    12301 agttataaat ccttatgcaa tttatttagt aaatagtaag ccatttatat tatttataga
    12361 gtcttctttt gaagaaaaaa aattacaaca aattagtaag cttatatgga atgctcaaat
    12421 accaattgct tttatatgcg attctaattc tgttaaaata tttaatggaa aagctttgga
    12481 tttaaagtca tatttaattg aggaaatata taatcaatct attgaggatt tttcggaaga
    12541 atctgagttt tcttatttga aaatatctga tccattattt tgggatcaat acactaaaca
    12601 atataccaaa acgcatttaa atgattattt gctagataat attacttcat tgacaaataa
    12661 attaaagaaa aaatataagg tgaaatttgc aacgaagctt gttctaagat taatatttat
    12721 tagatattta atcgatagag gtgtcgatat agcttataaa aattttaatc atgatattaa
    12781 agcttcacaa aaagaactgc taaagattac taaaaacaaa gttgaattat atgaactgtt
    12841 taaatactta aaagaaaagt ttaatggtaa tttatttgag ttaaatgacg aaattaataa
    12901 tacttgttta acagatgaag tgtttatttt actatctgat tttttatcag gtaaaataag
    12961 tattgacaat ggacaaatgt ctttattttc aatgtatgac tttaatatca taccagttga
    13021 actaattagt aatatatacg aaattctttt aggaaaagaa attcgtaata aagataacgc
    13081 attctatact ccgaattact tagcagaata tattttagat aaaacaacac ttgaatttct
    13141 gaaaaataat aggcaataca aaattctaga tccttcttgt ggatcaggag tatttttagt
    13201 aaatagtttt agacgtatta ttgatatgaa tttgggtgat aaactttttt gtgaagatga
    13261 taaactttta aaagaacttt tattatctaa tatttatgga attgatatca atgaagatgc
    13321 tatcgatgtt actattttct cgttatatct aactatttta gattacaaag accctaagat
    13381 attatcagaa tttaaactgc ctaatttaaa agggtgtaat ttaatagcga gtgatttttt
    13441 tgatgataat aaattggaga gtttaaagaa aattaacttt gattttatca ttggaaatcc
    13501 gccttgggga agtgtctcag atggattaca tttagattat tgcaataaaa atggctataa
    13561 tgatatgcaa caaaacaaag aaatcagtag aagttttatt tttagagcaa aagattttag
    13621 ctctagaaat acagtatgtt cttttgtagt gcattctaaa atactatata ctcaaaaaaa
    13681 gccatcaaaa aaattccgtg aatttttgct taaaaaaagt caaatattta gtgtagttga
    13741 aatgtcatct gtaagaaaat tagtgtttaa aaatgcagac gctcctgcag cagttatatc
    13801 atttaagtat aacgaaagtg aagaaaattt aaataataga atcatctata catctttaaa
    13861 accaaatgta ttttttaaat tatttaacat tattgtaatt gaaaaatatg atgttaaata
    13921 tatttcacaa aaactattat tggagaatga ttgggcttgg aaaactattg tgtatggatt
    13981 cagtaaagat atagatataa ttatgaatat caaaaaaaaa tatcaaactg taaatgaaat
    14041 tattttgaaa aacagttttg ttcatggaac agggatacaa atcaatggta atgaaagcca
    14101 tgatgcgaca catttaaaag gaaagtggta tgttgattca aagaaaggaa tagattcatt
    14161 ctatgttgat ataaataaag ggaaagaact tactcaagat aaaatgagaa gacctaaggc
    14221 taatcagcaa gaaatattta ttcctccata tgtattatta aaaaagggag ttgacacgaa
    14281 aacatataga tatagagcag cattttcaaa tgatgcattt gtatattcag atgcagttac
    14341 aggaatatgt ggaaaaaaag ataatttaac tacactttat tgcttgactg gtatgcttaa
    14401 ctcttctttt tactcatact taaatataat gctaggtaca tcaattggaa tagaaagaga
    14461 acaaggattt tcaacggaaa tttttacgta tccagcaata gaaagtgatg aaattgctaa
    14521 tctggtagta aaaatacaag aagcgactaa attagaaaaa acaactatgt tcttgaatga
    14581 aaaaaatagt tgtaagtata tcaaacaatt agataagtta attttgaagt cttttaattt
    14641 aaatgatgat aaatttattg actatgcaat aaatattcaa attcctgagc ttacaaattc
    14701 taagtcaact aacatatata gaaaaataac taaggaagat ttaattaatt atagtgaatg
    14761 tttcaaagaa cagttttcat cgatatatcg tagggttggg aaaagtattt caatttcggt
    14821 atatccaaaa atattaaatc gttttacagt atttgaacta gaagtaacag atgaaaaaac
    14881 agatagagag attatggtta aagcagaagc tgataataat atggagttgt ttacgaaaat
    14941 aattagatat gattataatg atgtttttca tcaaattaga gatattattc attttggaga
    15001 aaattcattt tatatcataa agcctaattg ctataaatat tggcatcctg ctatcgcaga
    15061 aatggatctt gctgatgttt tggatcagat tttatctgat acagggggtg aagaataatg
    15121 ggaggattta aaaattcttc actaaacttt tatttaaagc aagggcttta tgaaataatt
    15181 atagatggat taatagactg ttgtttactt atgagcaaat catgtattga acaatctaga
    15241 aaaatattaa atcacgaaga aaagataaga acgcatttag ttgaaaatta tttagaaaat
    15301 gatattgaaa gaagaaaaac atctttatct aaacaacatc taagatttga agtagaagca
    15361 caagaaaatt atgacaacaa aaatgaatct tatgagggta gggttgatat aaaagttata
    15421 tctaaagaca ctttagcgga tagaagggct tattatatca ttgaatgtaa aaggatagat
    15481 ggtaaggaaa ctttaaataa aaaatatata accgaaggtg tacaacgatt tattactgag
    15541 aaatattctt cgcattatgg caaaaatata atgcttggat ttatagtaaa agatataaat
    15601 atccagagca atatacaaaa aattaattca ctgaatcaga acatattata tacatatata
    15661 gatacagata tgttgacagt tagaagtgat tctgaaaata attatgctgt ttgtgaaagt
    15721 aaatataaag taaaatctaa actactattg ttaaatcata ttttttataa cttttcgagt
    15781 ataataagat aatcttatga atataaaata ttactatgtt aaaattatag tttggttttg
    15841 ataatattat aaaaataagt ttaagaataa aatataaagg aaaaaagggg cgtagcacta
    15901 ttttttagtg tgacagcccc tttaatttta aaatcggtaa ggaggagtgt atttgaaaaa
    15961 aattaagtca tatacaggga ttttgaatgt ggagaaggtt atatatgcaa taaatgattt
    16021 tcagcttcca tttccagtta cttttacgca gatgacatgg tttgtattaa cactttttat
    16081 aattatatta tttggtaagt tacctccatt atttatgata gaaggagcat ttttaaagta
    16141 tcttggcata ccaattttta taacttagtt tatgtctcaa aagacttttg atggaaagaa
    16201 accatatgga tttttaaagt caataatact atattcatta agacctaaaa taacatacgc
    16261 aggaaaaaac attaaattta aaaataaaat tattgatgaa aatataacta tagtaaggag
    16321 tgaattatat gttcccgata aaatatatag agaacaatct tgtatttaat aaggaaggag
    16381 aatgttttgc atattatgaa ttaataccat ataattattc attcttataa gctgaacaaa
    16441 aatttattgt acatgataat tttaggcaat taatatcaca aagtagaaat ggtaaaattc
    16501 atgcacttca aatagccact gaaagttcta taagagttac acaagaaagg ttaaagaaaa
    16561 atattacagg aaaattaaaa gaaattgctt atgaaaaaat agacgagcaa acagaggcac
    16621 ttgtttctat gattggcgat aatcaaattg attatcgttt ttttattggg tttaagcttt
    16681 tagttacaga gaaagaaatg aatataaaga gtataaaaaa gtcaatatta gctaatctat
    16741 cagattttat atatgatgtt aataataaat tgatggatga ctttgtcagt ataaataatg
    16801 atgaaattat gagattttta aaaatggaaa agttattaga aaataaaatc tcaagaagat
    16861 ttaaagttag aaggttagat aaaaatgatt ttggatattt aattgaacat aattatggaa
    16921 agaaaggcac agtttttgaa gattatcaat accatttacc tacaaagaaa cttaaaagag
    16981 aaacattagt aaagaaatac aatcttataa agccaactag atgtcttata gaagagaagc
    17041 aaagatactt aaaaattata catgaggatt cagaaagttt tgtagcttat tttactattg
    17101 aaattattat tggagaaatg aattttccat ctagtgagat attctattat cagcaacaac
    17161 agtttgcatt tcctattgat actagtatga atgtggaaat tgtaacaaat aaaaaggctt
    17221 taactacagt tagaaataag aaaaaagaat taaaagattt agacaatcat gcttggcagt
    17281 cagggaatga aacagtaaca aatgtagtag aagcattaga ggctgtaaat gagcttgtaa
    17341 ataatttaaa tcaaacaaaa gaatcaatgt ataagttaag ctatgtaatt agggtatcag
    17401 cagatagcat tgatgaatta aaacgccgtt gtgatgaagt taaagatttt tatgacgatt
    17461 taaatataaa attagtacgc ctaatgggag atatgatagg atttcacaat gaatttatac
    17521 cttcaagcaa aaggtatata aatgattata ttcaatatgt aactagtgat tttctagcag
    17581 gattagggtt tggagcaact caatagcttg gagagaacga aggaatatat attggatata
    17641 gtttagatac aggaagaaat gtctatcttc aaccatctct tgcaagtcaa ggagtaaaag
    17701 aatctgtaac taatgcgttg gtagcagcat tctttggatt tttaggaggt gggaaatcat
    17761 ttgctaataa cttacttgtt tactattcag tattgtttgg agggcaagct gttttggtgg
    17821 atccaaaagc tgaacgtggc aaatggaaag aaaatttacc agatatatat catgaagtaa
    17881 atattgttaa tttaacaagt gatatagaaa ataagggaat gctagatcct tatgtaatta
    17941 tgaaagaagt aaaagatagt ggaagtctag ctattgatat attaactttt cttacaggaa
    18001 taagtagtca tgatggagag cagtttccta tacttagaaa atcaatcata aatgttactc
    18061 aagacaaatg cagaggactt ttgaaggtaa tagatgaatt aaggaaagat aattctcaaa
    18121 tatcaaataa tattgcagat catattgaaa gttttacaga ttatgatttt gcacaattac
    18181 ttttctcaga tggaaatatt aaaggctcaa ttaatctaga aaaacaaatg aatataatac
    18241 aagttgcaga tcttgtttta ccgaataaag atactttatt tgaagaatat acaacaatgg
    18301 aaagtctttc agtagctatg ttgatagtaa ttagtacttt tgcattagat tttatccata
    18361 cagatagaag tacttttaaa atagtagatt tagatgaagc ttggacattt cttaatgtag
    18421 cacaaggaaa aactttatct aataaattag ttagagcagg atgtgctatg aatgcaggag
    18481 tgtattttgt aactcaaaat gcaggtgatg tatcagatga aaatttaaaa aacaatattg
    18541 gattaaaatt tgcattcaga agcacagata tcaatgaaat taagagaata cttgaatttt
    18601 ttggagttga taaggaagat gagaataatc aaaaaaggtt aagagagtta gagaatggac
    18661 agtgtttaat acaagattta tatggacgag ttggggtaat acaaatacat ctagtttttc
    18721 aagaactcct tactgcattt gatacaagac cacctataag aaaagaggtg gattaatgaa
    18781 atcgaaagta tttagaaatt taagctggat aactatttta atagtagtac ttttactatt
    18841 ttttatttat ataaatttat agatttagag aaacaaatag atacattaga taagaaaaga
    18901 aaaaaactca tagatatgag attagaagat ataattgata aagaatcata tgaagaaaag
    18961 tatgatgatt taacagttga aataaagaaa cttagtaaag agaaaaaaac tcttaaagca
    19021 tcattaataa atgaaaagag tataaaagca agacttagag aatttagaaa gatacttgaa
    19081 agaaatgaat tgttagaaaa atttgataga tatgtatttg caagtatagt agataaggtt
    19141 atagttggag ttattaatga agatggtata gttgatcctt ataaaataac ttttgtttat
    19201 aaaactagct ttgatgataa acaagatgga cataaattta taaataagag tccgttagaa
    19261 acgtattcaa aagataacaa cgataataaa aagaagtgtt catatactaa taatgagaca
    19321 ttgcgagact gtagttaaac ttattagaga ataacaaggt ttcagacttt tatattgtgt
    19381 cttaaaagta gatttgccac ctatttgcca ccataattat ttttgggtgg caaatttatt
    19441 ctacaatgat ttcaaaaata tttaccgtct ggtttctcat gttgttagtt acatgtaaat
    19501 atgtatccat agttgttgaa gttttagcat gtccgagtat atgctggata tcttttatat
    19561 tagtaccatt ttctaataac atagtatcag ggtttttaaa ataaagtgtg tattcttcta
    19621 aagtagtgat aattaaaata tcaaatatca ctatacaaag gaaagtgcac gctttttatg
    19681 tttaacaaaa acgaaatatt aaaaaactta ttgaagaata tgatgtcaaa acaactacag
    19741 atattcaaga tatgctaaaa gatttattta caaatacaat tcatcaaata tttacattgt
    19801 cgaattttag cggtttaaac ataatatatt gactgatata ataaaatcat agaatatttt
    19861 agggggaaat agattatgtt taagaaaaag ttaatagctg ttgcaatgag tataactatg
    19921 attagtgtag ggagcttttc gtatgctcat tcaggaagaa ctgattcatc aggaggtcat
    19981 cgtgataata aaaataaatc tggattagga agttatcact atcattgtgg tggttatcca
    20041 gctcatttac ataataatgg tggttgtcct tatactggag gaggaagcag ttctggaaca
    20101 acaacttcgg ttaataatga agaaaagcaa aagaggtctg ttggagaaaa aggatataat
    20161 caagggtatg aagatgggta taaaggaaat tatagttcat caaattatag tggagattat
    20221 agtgatacat atgaatcaaa gtactcagaa ggatatgaaa aaggtaaggc taaattagaa
    20281 gaggaagaaa aggtggctaa agagactgga tataatttag ggcttacagg tgctaaatct
    20341 aataatacat atgaaaaaga agttttaaaa aatgcttatg ataccggata ttcaactgga
    20401 tacaatgaat ataagactaa aaagatagaa gaatataaag ctaaagggat agaggatagc
    20461 aataaagata aggaaaaaat gacatttgaa gaaaacatag attctgaatt tatagatgct
    20521 tataataatg cttatgacga aatacaagaa caattaaaaa atgattacac tactcaagga
    20581 tttgagagtg ctatcaaagg agaaagattt gacacatcaa ctataggtaa tgttaaatat
    20641 gctaattggt ttaaagaagg atatgataat ggtaaggtta agttacctaa agcaaaagaa
    20701 agtgtatata atcaaggtta caatgaagaa gattttagta taccagatga aatgaagtct
    20761 attgaaacta agttaaaagg tgtatatgat gaaggtttag aaaaaagaga agaagagaaa
    20821 agtagaaatg ctacttatgg ggttggagct ggaacagcag ctgtagttgc tggagtagta
    20881 tataagaaga aaaaacgtaa aaagatatag atgctagata taccaatgat gacaagtgta
    20941 taacaaatat aattttaagt ataaatattt aaaatataga agggaaggtt atatctatgg
    21001 gatttaactt taggaaaagc ataaatctag gaggaggact caaattaaat ttaggtaaga
    21061 agagtgtagg tataagtgct ggtgttaaag gtgctagagt gtcagttaat tcaaaaggaa
    21121 gaaaatcagc tacattgagt atacctggaa ctggaatatc atatacaaaa actagtacaa
    21181 agaaacgtaa acctaaataa atatttgata tactaagaaa tagactgtta caaatagtct
    21241 attttttagt atatagattt aaaaagtgac aaaattttga aatataaact aatttcataa
    21301 aggaaaatat tattttatgt agaattataa tatttaagta atattataaa atttatagga
    21361 ggatttcatt atgaaattaa gcaaaatact attggcaata gggatgtctg tttcattatt
    21421 gattgctagt tgtccaacat caaacgctct tagttctatt aattcaataa aaggtacaaa
    21481 taagtatgaa acagcaggtt taatagcaga taaacaaaat tatgatattg caatacttat
    21541 taattctgaa aatggattat ctgatggatt gtcagcaagt ggattgtcag gagctaaaaa
    21601 tgccccaata ttgttaacta agaaagatac aatcccttca gaaacattaa aaagattaga
    21661 aaaggtaaaa gaagtatatc taattggagg aaaaaattcg ataggagcat cagttcagaa
    21721 aatattaagt gataaaaaaa taagcataaa tagaataagt ggaactgata gaatagatac
    21781 aagtcataaa gttgcaaaag aaataggaag agtaagtacg gttaaaaaag taatattaac
    21841 taatgctttt aaaggcgaac cagatgctat gagtgttgct tctatagcag ttagagataa
    21901 atctccaata gtattaactg atggaaatac tacaagtttt gctacagatg gattagaaac
    21961 ttatgcaatt ggtggaaaag tcagtatgag tgataagtta ataaatgaaa ctaaagcaac
    22021 aagaataggt ggagtagata gatacgatac aaataaacaa ataataaata aattttataa
    22081 tggctcaaaa gaattttaca tagcaagtgg gactgattta gtttattctt tagttggttc
    22141 tacaattgca caaaaaacac ctatagtgtt agttggaaat ggaagtaata agagtatatt
    22201 aaaaaatgca agtaaaataa ctgctattgg aaatattgat gatgttgtta taaaacaatg
    22261 tttaaatata actaataatg ttggggattc aaatacaggt attgtaaaag atactaattc
    22321 aaataatacg aacaaaccaa gtaatgagcc tagctctaat aatcctggat taactgataa
    22381 aagtacagta tatgtaactc ctaatggaaa atcatatcat ctaacaaaaa attgttctac
    22441 actaaaaaga agtaaaacaa taaataaagt aacattaaaa caagctaagt cacaaggtaa
    22501 aatagatcct tgtaatgttt gtgtaaaata atgtattagc caaatatata ttaaaaaaga
    22561 caattaatta agattgtctt ttttattgaa gaaatataaa tttatattat gtattattaa
    22621 aaaaaaatta ttaattataa aaaataataa taaagacgat aagtattaaa attttatgtt
    22681 tccaatattt atgatataat taatatggca aggaataaat atttgaaaaa gctatgagtg
    22741 gtgtttccat ggaagtttct tcactttctt atgagaggag gtgaaaacta tggaaaatct
    22801 aatgatgagt attataactg gtgttatagc cagttacatt tacgataaat taaagagcca
    22861 ctctgagcga ccaactaaga gtggctggga acttaatata aagttccaga aaagtaaatc
    22921 ttcaaaataa tttgttgaag gaaacatcac tcaaagttaa gtaaaatatg tttccttgct
    22981 tttattatat cacaaattaa aaatattaac acaaaatttt gtgaatatat acttattatt
    23041 gttctaaaaa aatatgtaaa taattgattt gattgcatgg aattattgat gtaatattta
    23101 agttaaatat acacaatctc aattaaagaa gataagtact ataaattcat atcaaaaaaa
    23161 tacataaact tttaagttat aattttataa aaagtctttt agtatcatta tttcaagagg
    23221 aattatcata tgaaaaaata acttcattag aaaagtggat aaaaaactgg taagaagatg
    23281 actaaagatt acatatacta ttacttaaat taaaaattta atataattta taatgtatac
    23341 aatttattat aaaaaattt
//
LOCUS       DAYAOX010000043        23165 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.043, whole genome shotgun sequence.
ACCESSION   DAYAOX010000043 DAYAOX010000000
VERSION     DAYAOX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.043"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            135..1367
                     /locus_tag="ACGI57_001327"
     CDS             135..1367
                     /locus_tag="ACGI57_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFG9716408.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            1381..2187
                     /locus_tag="ACGI57_001328"
     CDS             1381..2187
                     /locus_tag="ACGI57_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HFG9716409.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2299..3168)
                     /locus_tag="ACGI57_001329"
     CDS             complement(2299..3168)
                     /locus_tag="ACGI57_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9716410.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3540..4967
                     /locus_tag="ACGI57_001330"
     CDS             3540..4967
                     /locus_tag="ACGI57_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HFG9716411.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVEIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5044..5313)
                     /locus_tag="ACGI57_001331"
     CDS             complement(5044..5313)
                     /locus_tag="ACGI57_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716412.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5316..6758)
                     /gene="nhaC"
                     /locus_tag="ACGI57_001332"
     CDS             complement(5316..6758)
                     /gene="nhaC"
                     /locus_tag="ACGI57_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFG9716413.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(6783..7949)
                     /locus_tag="ACGI57_001333"
     CDS             complement(6783..7949)
                     /locus_tag="ACGI57_001333"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9716414.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(8174..10294)
                     /locus_tag="ACGI57_001334"
     CDS             complement(8174..10294)
                     /locus_tag="ACGI57_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9716415.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQNFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            10527..10928
                     /locus_tag="ACGI57_001335"
     CDS             10527..10928
                     /locus_tag="ACGI57_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716416.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            10971..11264
                     /locus_tag="ACGI57_001336"
     CDS             10971..11264
                     /locus_tag="ACGI57_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFG9716417.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            11279..11719
                     /locus_tag="ACGI57_001337"
     CDS             11279..11719
                     /locus_tag="ACGI57_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HFG9716418.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11811..12284)
                     /locus_tag="ACGI57_001338"
     CDS             complement(11811..12284)
                     /locus_tag="ACGI57_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFG9716419.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(12284..13072)
                     /locus_tag="ACGI57_001339"
     CDS             complement(12284..13072)
                     /locus_tag="ACGI57_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFG9716420.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            13400..14644
                     /gene="glyA"
                     /locus_tag="ACGI57_001340"
     CDS             13400..14644
                     /gene="glyA"
                     /locus_tag="ACGI57_001340"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFG9716421.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALITSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            14967..16064
                     /locus_tag="ACGI57_001341"
     CDS             14967..16064
                     /locus_tag="ACGI57_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9716422.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            16055..16753
                     /locus_tag="ACGI57_001342"
     CDS             16055..16753
                     /locus_tag="ACGI57_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9716423.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            16756..17820
                     /locus_tag="ACGI57_001343"
     CDS             16756..17820
                     /locus_tag="ACGI57_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFG9716424.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            18003..18707
                     /locus_tag="ACGI57_001344"
     CDS             18003..18707
                     /locus_tag="ACGI57_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9716425.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            18700..20022
                     /locus_tag="ACGI57_001345"
     CDS             18700..20022
                     /locus_tag="ACGI57_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFG9716426.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            20209..21582
                     /locus_tag="ACGI57_001346"
     CDS             20209..21582
                     /locus_tag="ACGI57_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716427.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(21954..22892)
                     /gene="pdaA"
                     /locus_tag="ACGI57_001347"
     CDS             complement(21954..22892)
                     /gene="pdaA"
                     /locus_tag="ACGI57_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HFG9716428.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         8529 a         2885 c         3670 g         8081 t
ORIGIN      
        1 attgataaca aactagatat tatacttttt tgtagaatag aattacaatt atataaagaa
       61 aaattctatt aatagtgaaa aaaaagtaga aaaaagtaga aattattctt ttataaagaa
      121 agggagggat aatattgcaa gagagagaag aattagtttt aaatccagtt ccaaaagagg
      181 aaagagttgg atggttagct ccattgttta atatgcttgg ttctaacata gctatatctg
      241 aactaatggt tgggggaact ttaattttag gaatgactct ttctaatatg attattacat
      301 caattatagg gaacttaata ttagttgcaa ttattatgat acaaggttat ataggttaca
      361 aagaagggtt aaatacgtat gtattagcta agggggcatt tggagagata ggaggaaagt
      421 atcttatatc tctattactt ggtataacta gttttggatg gtttggtgta caagcagggg
      481 ttgcaggact atcagtacaa aagatattcc caagtgtaaa cctaacatta gttacagtta
      541 tacttggatt atttatggtt gtttttgcac tgtatggttt taaagctatg gctaaattta
      601 attacttagt tataccacca cttatgatat taatggtgtg gggagttttt aaagcttttt
      661 ctacttatgg agttgaagct atttataatt atactcctca aacaactatg agtatggttg
      721 aaggtcttaa tattgtagta ggtcttgtta tagttggtgc aattatatca cctgaccagt
      781 taagatatac gagacgagta aaagacattt ggattatcag ttttttaggt ctagggatta
      841 tttcattatt ccaacaagtt gcagctggtg taatgtctat gggagcacct acttgggata
      901 taacagaagt attggctaat ttaggttttg gatgggtagc gtttgttatt ttaatacttg
      961 catcttggtc tacaaacgta tcaaatgctt attctggtgg attagcatta aaaactattt
     1021 ttccaaatgt aaaaagaaat gttttgactt tagttgcagg tctaataggt acaataatag
     1081 cagctactgg tataatattt aaatttcagt catttttatc tttcttggga atcgcagttc
     1141 ctgcaatagc aggaattatg tggtgtgagt attattttat acaaggaaga acttataagc
     1201 atagagaagg aataaattgg attgcagtaa taagttggtt aataggtttt gcagcgtcgt
     1261 attatagttc aaaaataaat ttcttaatac ctccgataaa tggaattgta gtgagtatgg
     1321 ttatttatta tatattaatg aattgttttg gaatcaaaga taaataaagg gggcaattac
     1381 atgggtcgta aggtgaaaat aggtataatt caacaacaca gtgtacttgg aaatgttaaa
     1441 aaaaatatag aaaaagcagt agagatgata gatgacttag gaaaacaagg ggcagatata
     1501 atttgtttac cagaactatt tgctacagga tataatttag agtctttagg tggagttaaa
     1561 acattagaat taataagaga acataataag tacatagagg aatcaatgtc agaagctgct
     1621 aaaagaaata atgtatacct gatatctcca tatggaacct tagaaaaagg gtctacacat
     1681 gtttataatt cagcagttat atttgataga aaaggtaaga ttatgggaga atactgtaaa
     1741 aatcatctgt ggtcattaga agcagtatac tttaaaggag gggaaaaagt agaggtatat
     1801 gatgctgatt ttggtagatt tggtgtaatg atatgttatg atgcaggttt tccagaagta
     1861 tccagagaat tgacattaaa aggttctgaa ataatattta taccatctgc atggagaata
     1921 caagatgaag atatgtggga tttaaatgta tctcaaagag ctttagagaa cactgtatat
     1981 acagtaggtg taaatttagt aagtaatgac tctaatttaa tactatttgg aaagagtaaa
     2041 atatgcaatc ctagaggtac tgtaataact caattagata catacaggga aaaatatgta
     2101 ttatcagaga ttgatttaga tgaaataaaa aagtataggg aagatatccc ctatttgaaa
     2161 gacagaaaaa gtagctataa catataattc tatactataa tacaagttca atataaaatg
     2221 ataaaaagag gatgtatcaa tattaagatt aaatttatta tactttgata tatcctcttt
     2281 aatattatca actttgattt aaatattttt aaaatagttg ataagaaagt ttttgaacaa
     2341 caacacagct ttagattcat agttttcgct aaaccatgaa agatttaaat atcttttaaa
     2401 ttcaatatct ttgatatgta acaacttaat ctcagggtct tgagataatc cccatgattt
     2461 tccacatata aatccaactc cttgaagtga ttttataaga gcataaattg tatatggtga
     2521 atcagactca aatgctattt tgggtttgaa attagctgat tcgcacagga tatcaattac
     2581 ctctctataa ttctcaccct tcgttataac tataaagttt tcatctgaaa cttcgcttag
     2641 atatacttca tcctttaatg ataaaggatg gtttattgaa actcctaata gtatttcttc
     2701 gcatgttaaa gttatgctat tttctgagtt tagctttgta aatgaagaag atatgtacaa
     2761 atcaaaatca ctttttttat aagatgatgg caaagtatgt gacacattaa aagtaatatt
     2821 gggatatagt tttctgaaat tactcaaaag tgatggtaac acattagccg ctgcaggaga
     2881 taatatcttt acttctcctg aattttccaa agaagtgtct tgtaactcct tttttgaact
     2941 ttctactaaa tctaaaatgc tatctactct ttttagaaac aatcttccat actcatttaa
     3001 aacaatatat cttccttttc tgtcaaataa ctgaactcca agttcttttt ctaaggaaga
     3061 tatagttttg cttaaagctg gctgtgctat atttaacttg tgtgatgctt ttgttatgtg
     3121 ctccattcta gcaacagttt gaaaatactt aagatgcaat aaatccatag tataaaatac
     3181 acctctttta atatgacttg agttatatca attttataat caactccaca ttatacatta
     3241 taatataaag tttatataat aaactacaat atattaaaaa gttatttaaa ttgttattta
     3301 tattttgtac ataatctaat taaaaaaagt aactttaaat ggttaactaa ggaaatattg
     3361 ttacaaactt tagttatata aaaaattcgt ttgatgagca aagtattaat gtcaagtaag
     3421 aataaaagct tattagataa gaaaaataat attttataat aatgatgtta cttaaattat
     3481 tatgttatac tattttaaat aggaaacatt tagatactat aattttaggg ggagtcaaga
     3541 tgaaattaac taaatcaata tcatcaatgc tgtgcttatc tctagtttta agcttatcac
     3601 cagtagcata tgcaaatgaa ataagtagaa caacagttga gaatacttta gtaggtcagg
     3661 atagatatca aactgctgtt gaagtaagta aaaaaggatg gagtagtgca aatgaggctg
     3721 tagttgtaaa tagtgaatct attgtagatg cattagctgt aacaccattt gctaaattaa
     3781 aaaatgctcc tatattattg acagaaaaaa ataatctaaa ttcacaaact gagaaagaat
     3841 taaaaagatt aggagttaaa aaagtttata taattggtct atctagttct gtatcaaatg
     3901 atgtgcaaaa acagttagaa tctaataatt taactactga tagagttggt ggtagtgata
     3961 gataccaaac agctttgagt atagctaaaa aaatagaagg attaaaagat atatcagaaa
     4021 tagctgtagt aaatgggtac acaggtcttg cagatgcagt tagtatagct tctgtagctg
     4081 ctactaatgg aatggttata cttccagtat ctgatgcatc tggtatatct agctttaaag
     4141 attttataga atctaagaat ataaataaat catacataat aggctctact aatgctgtat
     4201 cagataaaat aaaacaaagt ttacctaatt cagaaagaat aggtggagca gatagaaatg
     4261 aaactaatgg taaagtaata gaaaagtttt atacatctaa taaattaaac aatgtatttg
     4321 tggctaagga tggaatgaaa aatcaaaatc aacttataga cgctttagca gtaggagtac
     4381 tagcatcaaa agaaaattct cctgtactaa tcgtaggaga acaattaagt gcaacacaaa
     4441 agagtgtatt ttcgaataaa aatgctacct ctataactca agttggtagt ggaggaaatg
     4501 aaaatgcatt taaagaatta aaagatttac aggcaaatag taacaataag agagtaatgt
     4561 atgtaactaa tacagataag gtaaatataa gaaatgatgc tacaatagaa gcttctgtta
     4621 taggatatct aaataatggt gatgaagtag aagttttaga tgtgttaaaa actggatggg
     4681 tagaaataaa atacaatgaa ggtattggat atgttagtgg gtcttatttg acaaataata
     4741 agcctgataa tagtaatgaa aatataaaaa taaagtatgt aaaagaaaaa gatggtctaa
     4801 atgttaggaa aggaccaagt acagaagatg aaaaaatagg tcacctttca tatggaagca
     4861 aagtagaaac aatagaaatg tttgctactg gatgggtaaa aataaagtat aatggtggat
     4921 atggttatgt aagtaatgat tacttatctg atacaccagt tatttaatta taagtaacaa
     4981 taaaaaaact gctttaattt gtaaagcagt ttttttataa aaatttaagt ctaataacta
     5041 tttctatttt gataccttaa tcctgggatt tattattatg gatattatca acatacaaac
     5101 actccaaatc cagtgtctaa ttccaaactt agatattaat aaattcagtc ctgctatggt
     5161 aaataagcag tatttaatta caattaaaag tgtatttata gaaagcccct tcatagaaag
     5221 tatcctaaat ccaataatag acattctaag tcctataaat gccaataata tacctgcaat
     5281 atcctgaatt aagtaatagt attcaaattt catattcaat caactcttct ctgagcagta
     5341 tcaaatattt ctaactcttc aatttcatca tctgtgtatg taagcatagt aaaaccagtt
     5401 acaccataaa taaatgaaat tataggtgta atccaactta agaaacaata tggaataaat
     5461 tctagtgggc ttacaccaag agtctgagca cagaaaacag catttgaatt ccatggtatt
     5521 aatggaccac caagtgtagc tgtatcttct ataattctag atagattttg tggttttaat
     5581 ttaaattctt tgtatattgg agacattaga gttccgacaa atacagaagt gaacatcatt
     5641 gatgaactaa atgcatttgc aaagtaactt actataaaag tagacccaac caatttagtt
     5701 cttgattttc ctattttagt tataagaggt tgaagcagta cttctaagaa tccagttttt
     5761 tggagaatac cagcaataac gaaaactaca aatactagta aaacggtttc agccatactc
     5821 attattccac cacgatttaa tagtttgtcg gcatagacaa atccagtatc tatagtaaat
     5881 cctgatagca tgtaatttaa aacatcacct attttttctc cttcttgaaa tactgctata
     5941 ataagtccca tgatagctga acttagtata gatataatgg gtggcttttg tagcaataaa
     6001 agtaataata aaaatattat tggaatcatt gctactaaac caatatgaaa gtttgaattt
     6061 aatacatcct tgatttgatt tatttgaaca tagtctatag tattatttga atacttaaat
     6121 ccaattactg tatacagaac tatacatatt acgtatgcag gtcctgtggt ataaaacata
     6181 tgcttcatat gtgtaattac atctgtctta caaactgctg cagctaagtt tgtagaatct
     6241 gatagaggtg acatcttatc tccaaagaaa gcaccagaaa tgactgcacc agctgttaaa
     6301 ccaagaggaa tacccatact agtaccaata ctcatcattg ctataccaga agttccaaga
     6361 gtcccccaag atgtaccagt agcaacagaa gttaatgaac atacgattaa tgttgtaagc
     6421 agaaaatatt taggggttat tattgttagt ccactatata ctactgttgg tattgtacca
     6481 gcagctatcc aagctgctat tagtacacca acagaaagta taattaagtt tgattgaaag
     6541 gcatctttac caacttcaaa tccaaaagct tctatttcat cattagtata accaagtttc
     6601 atagctgcag gaactactat aagccatgaa agtaaaaaca ttgtagttat agaagcatta
     6661 aatacaacta taccaactga tgttagagct attattgcaa gcattactaa caaggaatat
     6721 ctaaatgaag gtatttttct ttctgtattc attttcatag tgcaaattct ccttaaatat
     6781 ttttagttaa ttgcattttt tattcttata agagcttctt caagcatact tcttggacaa
     6841 gctaagttta ttctttgata cccacttcct ccaatgccaa agctgtttcc ttgatttaga
     6901 gcaactttac ctttttgtac aagtatactt tctagttcct catcacttaa tccaagtgca
     6961 ctaaagtcta cccataataa ataagtacct tctggttttc taactttaag ttttggcata
     7021 ttttcattta tatattttat agcaaaatct atattagatt ctaaatattc taaaaatgat
     7081 tctagccaag attcaccatt attatatgaa gcttcagttg caactaagct aaagcaatta
     7141 tttctcttta tatcaattct ggtaaaggca tcatctaaca atttatagtc tttttcatca
     7201 ggtagtatta catatgatga ttgaagacca gctatattaa aagttttagt tggagccata
     7261 caagtaattg tatttttttc aaattcttta gaaatagatg ccataggaat atgcttatgt
     7321 ttttttaata taatgtcact atgtatttca tcagatatga tttttacatt atgcttaaga
     7381 caaatatctc ctagtttttt taattcatct tttgtccata ctctaccaac tggattatga
     7441 ggattacaaa gtatgaataa ttttacatct ttaatcttgt tttcaatgtc ttcataatcc
     7501 attatgtagt ttccattttc tagtttttgt aatgggctta taattagttc tctattgtta
     7561 ttttttacaa cactattaaa agggctgtaa actggttctt gtatcattat tttatcattt
     7621 gcttttgtga gttcatttat taaaagactt atagctggga taacacctgg actataaatt
     7681 aaccactcac tttttatttt ccagttatgt ctcctatcta gccagtttac tatagattca
     7741 ttgtaagaat ctggtcttgt tgtgtagcca taaatctctt gttctagtct attttttaag
     7801 gaatcaatta tacaaggtgc agccttaaaa tccatgtcag ccacccacat aggaagtaaa
     7861 tcattagttc catacttttt ttccatttct gaccatttag atgaaaaatt attgcttcta
     7921 tcaacaatct cattaaaatt ataattcatt gtaaatacct ccagtaattt taattttatg
     7981 ttaataatat taagcaaaca acgtgccaat attgataata aaaaatattt attcacaagt
     8041 taatatttaa tattaagtaa tttcagagca tagataagat aaaaatatta attatactta
     8101 aaactttgtg gaaaaggtta tttctaataa atttttaaat atatgaaaat gtaattaaat
     8161 aggttaaata ggtttaactg tttaacttgt tagacctatt tatatttttt agaaattcta
     8221 ctccttttga tgttataatt gaacctccac gaccaacaag tatgtctaca agcccaaagt
     8281 tttgtagttc taaaagcata tttctaatct gttgttctgt caaaaatcta ttttcctcta
     8341 aagctatttt acataagctt tttctaccta ttctttcttt taaatcataa gcctttttta
     8401 aattatccaa tataaatatg tattcatcta gtgacctttt aaaattatag tcgtatttca
     8461 tgatgttttt gtctttgcca atgttgaatt gatacttttt tatattatca cttactttat
     8521 tacaaacggt tctactataa tttgaatcta tagtatctaa tatatattca ggtaaatcac
     8581 atatttctat aatatctttt tccaagtaac agaagtattc tcctaagttt cttaattctc
     8641 ttacatttcc ttcccatcta tacattttaa agatttcttt tacttcctca cttagaataa
     8701 aattacatgg aatatcatat tttaatgaac caaaaatttc aaatatatca gcaggtcttt
     8761 ccctaagtgg atgtatttta aggggaagaa cattcaacct aaaatataaa tctcttctaa
     8821 acatattttt agatacaagt tcttttaaat ttctattagt agctgcaatt attcttacat
     8881 caatgtcaat gacattatta gaaccaatac gtcgtacttg tttttcttgt atgactctaa
     8941 gtaatttaga ttgagaattt aaatccattt ctccaatttc gtctaaaaat atagttccat
     9001 tgttggctaa ttcaaataaa ccaattttcc cacctttttt agcaccagta aaagcccctt
     9061 catcatagcc aaataattca ctttgcagta gattttcttg aaaagtacta caatttactg
     9121 cgacaaaagg accatctttt ctcctagaag cactatgaat ggattgagca aataattctt
     9181 taccagtacc actttcacca attataagga tagatgaatt tgattgagcc attttattag
     9241 caattttttt tgtattaata gtttgtatac tagaacctaa tatgtcatca aatgtgtatt
     9301 tactgatatt accagagttt actagttgtg ctcttaattt agcttgcttt ttttctaatt
     9361 gagaaaactt tgtcatcttt agtataaatc catcaaatac atttaattgt atatgtttaa
     9421 tctctaaatt tatatcaata tgatttattt taatcaattt ctcaaaaaat ggagttttat
     9481 tggtcaatat gctttgaaaa ttgatgtctt ttatgcaatc accaacaaat gagtcaatca
     9541 tttcatttgc atttatagac aaaatttttc tagccatatg attatagaat tgaattattc
     9601 catcgttatt tgtgcatata attccatcat caattataga tagcaacaaa tcaaattgtc
     9661 tttcaaactt atttgcatct aacaataact cacctgtact ataacttgta ggaattattt
     9721 tttctacgta tttttttatt aggtcatctt ttattagatg ttttaatttg gtttttgtag
     9781 caatttcaac aatacaactt aaatctatta ttctgtattt taaatctata acttcacaat
     9841 ttttaaactt aggcaagatt ttttcgccag gagttaggat tacagcatct gtgggagtaa
     9901 actcaatctc tggatagcag ggcaggatat ttatattgtc tattcctaat ctatgtatta
     9961 aagaaattgt ttctattgcc atatctttac ttaagttata tacataaata attttatctt
    10021 tttctaattt ggatatcata tcaatactgt tcttttcaaa tgtcaactta ggaacaatga
    10081 tttgagcatc atagtgagat aattttacta tttcatcata ttttaaataa gcagatagga
    10141 gtataagttt ttctttaaaa aactttaaat ttccctcttc aaatgagtat aaatttatct
    10201 ttagattatc acctaagaat tgccttattt gattttcata aatttctcca gcgtcttttt
    10261 ttaaacttac aatagctaat tccatttgtt tcattttaac ctctcataca aatttgactt
    10321 attttagatt acataataat taaattagat taaataatat tatttaatta ttattataaa
    10381 tttattatag actacacatt gaagtaaaag aagtagctaa aattattcct tttatttttg
    10441 ttttttgtat ctatttggta taatagtatt agagcgtata ataatttagg caaaattttt
    10501 taaggaagac tagaggagaa tataaaatga aaaaatgtct ttactgtaat agggatataa
    10561 agtcaccaaa taataaatta gctataaaat atgatgacaa tatgaatata tatccatgtc
    10621 gtgcagaatg taaggaaaaa attgaagaat atatagcaaa aaaacctaca tataagttta
    10681 taaatatatt agaggttctt ttaggtgtgg gcggaatatt ctgctttttg agtaaatgga
    10741 ctattgcagc ccaagttgta cttggaattg atgcactggt tatattttta ttcccattgg
    10801 caggatttaa aatgaatgga aagttaagta tggaacaaac taagaatcaa agtaggtcta
    10861 ttgcaatatc aattttggca tttatagtaa taataactgt attatatttt aaacaagttg
    10921 gtaagtagag cataattgta taagacatca ttagagtatg aggtgagttt atggaagaaa
    10981 gaaaattagc accaaacaat gagagggaag ctctcataaa aagattaaaa agaatagaag
    11041 gtcaagttaa gggtatacaa aagatggtgg aacaagaacg ttattgtgtt gatatattgg
    11101 ttcaaatatc tgctataagg tctgctataa atagagtagg cacaattatc ttagagaatc
    11161 atataaaagg atgtgtagca gaaagtatta aacatgatga tgctgataaa actgaagaga
    11221 tgataaaaga gcttatggat actataaata aatttacaaa ataaggaggc ggttagttat
    11281 gaatcaacaa ggatttatta tggaaattgt tgatgataga actgcaaagt taaaaatgca
    11341 aagacattca gcttgtgctg cttgtggaaa atgtgctact acaaattcag cagagtcaaa
    11401 agaaatatta gtagaagtag acaacacaat tggtgcaaaa gttggtgacc atgtagaagt
    11461 gagtatggat aatatgaatg tattaaaagc agctgtaatg gcttatataa tacctcttat
    11521 agcacttctt gtaggaacaa tagggactta ttatatattg ggggcaattg gaatgacaac
    11581 tggagttgaa gccataagtg gattagttgg aatttgtctt acaataatct gttatttata
    11641 cttaaagaag aatgacaaaa aatttagaga cagtagagaa tttataccag taataacaag
    11701 aatattaatt gacctttaag ataattataa ttaaaaaata gcaatgtact ttttaaataa
    11761 atactcaata cgtatttgta gagtttaagt acattgctat ttttatattt ttattttgat
    11821 tttctgtaag gaataaagtt ttttctgatt tttaaaagat tcaatgttct tacaaaagaa
    11881 gttacaacta atacaccaag tgctattgca gcacttagaa atagcgcatc tgtacctttt
    11941 gacatagcca aagaataatt tttctgaatc aaagacaaca ttgtattata tagcccaatt
    12001 cctggtatta gaggaagaat acctggaatt acgaacacaa tagctggttg ctttagtttt
    12061 cttgctaaag tctcactgca cgctgagacc aaagcagcag cgataaaacc tgcaaaaact
    12121 gcatttgtag taaaattaaa taaataaaca tatacaatcc aaccaattcc gccagttact
    12181 ccagctggaa gaagtagata tagtggagca ttaaagaaaa cagcaaatcc agcagttgca
    12241 acacctgaaa ataaaaaatg caaaatcata gacatatcag tcattagaaa ggacctccta
    12301 ttctatacca tgtatcaagg acaaaaccaa ctccagaagc aattgcaata ataatcatag
    12361 cagcatcaaa agcacgagaa attcctgata ttaaattccc tgatattaaa tctctaatag
    12421 cttttataaa agcaactcct gggagtagtg gcattatagc gccgactata agcattttag
    12481 ggtcatctaa aatgcctatt tcaactagta gaactcctga aagtgcaatt aaaaaagaag
    12541 atactaaaca ggaaaatgca gatattgcac ttatcttcat cgttttatca taagtaattg
    12601 cagcaaagat tgctattata aacgtaagta taaaatttaa tacataattc cctccaagca
    12661 aacaagcaaa acaagcagat gcaattcctg tacaagagta aaataaccat aaaggataag
    12721 gttttacatc ttggatttca taaagacgtg caatggcatt atcaatatca tactctttgt
    12781 cactaacaaa ttctcgagaa aaactgttta ataaagaaat cttatttaag tttatacctc
    12841 ttgattgaat cgttttcata aacgaaaagc catcaaattt ctcatctgaa ataattatta
    12901 ctgtaggagt tgtaaaaata tttacatgat aaaaccctct tgacctgcat attctaagga
    12961 cactgtcttc aaccctagat gtttcagctc cattttttat catcagttcc cctacaaata
    13021 gagctagttt tagaatattt ttcttatatt caagttcctc attttgattc ataaatacac
    13081 ctccaaatta aaaaacgtaa tattgaaatt atatcaaatt tttaagtaat attccttaat
    13141 atctatacaa atatattatc ttttttttgt ttcaaaatag tcacatttaa attatatcca
    13201 attaaaagaa ttagacaata ttatttatta tattttaact taaaaataaa gaaagtaact
    13261 atgttaaata tggaaaaatg gcaaaacact tgactattaa ttggtaaagg actataataa
    13321 taaattatca tattgataaa aacgaaaatt atgaaatgtc tgtaaaaata aaatttaaat
    13381 aataactagg aggagtaata tgtttgagaa cttgaaaaaa gcggaccctg agatttatga
    13441 aagtatgaaa agagagttaa aaagacaaca aagaaatatt gaattaatag catcagaaaa
    13501 catagtatca gttccagtaa tggaaactat gggaagtcat cttacaaata agtatgcaga
    13561 aggttactca ggcaaaagat attatggtgg atgtgaatac atagatgaag ttgaaactct
    13621 tgcaatagat agaatcaaaa aaatatttgg agcagaacat gctaatgttc aacctcactc
    13681 tggagcaaat gcaaatattg gtgtatattt tgctatgtta aaaccaggtg atgttgttat
    13741 gggaatgaat ttatctcaag gtggacattt aactcatggt gctccagtta atatttctgg
    13801 tcaatattat aaattttatg aatatggtat agataaagac agtggactta tagattttga
    13861 tgaagttaga aagatagcac atgaagtaaa acctaaaatg atagttgcag gagcaagtgc
    13921 atatcctaga gaaatagatt ttaaaaagtt tagagagata gctgatgagg ttggagcatt
    13981 gcttatggta gatatggctc atatagcagg tcttgtggct gctggtcttc accaaaatcc
    14041 atgtgaagtg gctgattttg ttacaacaac aactcacaaa actttaagag gacctcgagg
    14101 gggtgttatt ctttgtaaag aaaaatatgc taaagacata gacaaagcaa tattccctgg
    14161 tattcaaggt ggacctcttg aacatataat tgcttctaag gcagtttgct ttaaagaagc
    14221 tttaagtgat gagtttaaag aatatcaggt acaagttgct aaaaatgcaa aagccttagc
    14281 tgaagaactt attaaaagag attttaaatt gatttctgga ggtacagata atcatttaat
    14341 actattagat ttgactaata aaaatataac tggtaaagca gcagaaaaga gattggatga
    14401 tgcttatata acagctaata aaaatacaat tccatttgac ccaaatggtg ctcttattac
    14461 aagtggaata agacttggta ctccagctgt aactactaga ggtatgaaag aagaagatat
    14521 ggcaataatc gctgaggcta tagacctatg tttaacttat gatgaagaat ctaaggctag
    14581 aacattggtt gttggtttaa ctgaaaaata tcctttatat gaagaatata atattatgga
    14641 ttaattataa agaacaacta aataaaatac atttaaataa ttacaatgat tgtgtatgta
    14701 tttgaatata gttagatata aaaaaggtgt ctcaaaatga actttttagt ttatgaggca
    14761 cttttttgta tggaatcgaa catatttgtg ttatttaaat aataaaaaat atggttatct
    14821 ctatttttag atggtctctt tgtatttttt aattaaaact ttaagatttc ttaagaatta
    14881 tactaggttt ttctaaagat ttaaagtcta tacttataag tatagtaatc atatgaaaag
    14941 aaaagcttta acgaggaggg aatcttatga aggtattaat tcttacagga aaatttggta
    15001 tgggtcatta ctcagcatca aattctttaa gtgaagatat aaaagcaaaa tttgataatt
    15061 cagaaataat aattaaagat atatttgagt acattatgcc taactattca gataaaatgt
    15121 acaagacttt ttctattctt gtaaatcggg gaagtagtct ttataattta ttttataaat
    15181 gtgctgaaaa tggaaagaaa gatataaaat ttactttttc agattatttt ttaaataaat
    15241 tagatacatt acttcatgaa gtacaaccaa cagtagttat ttcaactttt ccattttgtt
    15301 ctcaattagt atcaagatat aaggaaaaat ataactcaaa tcttccttta ataacatgta
    15361 taactgatat aagtagtcac tcagaatgga ttagtaaaaa tacagactgt tatttagtag
    15421 ctagtaaatc tactaaggaa gaacttgttt ttaaaggaat agatgaatct aagattaaag
    15481 ttaatggtat tccagtaaaa aaagaattta aaagaataga acatgtaaat cattcaacta
    15541 aaaaaaatat tcttataatg ggtggaggat taggattact gcctaaatca gaacaatttt
    15601 acaaagaatt aaatagttta gaaggagtaa aaactactgt aataactggt aataataaaa
    15661 aaatgtacta taaactatat ggtagatatg aaaatataga ggtagtaggt tacaccaatg
    15721 aagtatataa atatatgaaa gattctgacc ttattatttc taaaccaggt ggaataacac
    15781 tatttgaaac aatttattca gaacttccaa tattggcatt taatccattt ttacaacaag
    15841 agatagataa tgctagtttt atattaaata atgaaattgg gagaatactt ggtaaaaata
    15901 aaaaatacta tgtagatgag ataaaagatt taatttatga tgatgctact cttaaagaaa
    15961 tgagcagtaa catgaaagaa ttaaaaaaac aatttgataa taatacctta gagaatattt
    16021 tattttcact tgatgaacaa ggagcgtgta gagaatgtat gtagtaggtt taattatcgt
    16081 tgttgcattg atttttttag tccattcaat tatacctaca tactataata aactattaaa
    16141 taaagaggta ttaaaaaata tggctggaga aaatgaaatt gcacttacat ttgatgatgg
    16201 tccagataag agatatacag aaaaattact agatgtgtta aaagaaaatg atatacaagc
    16261 tatgtttttt gtagtagcaa agaatgctga aaaagagcct gaaattataa aaagaatgtt
    16321 gagagaaaat catatagttg gtttacattc attggaacac agaaatgcat ggctttatag
    16381 ttatagttat gttaaaaaag attttattga aagtacaaat ataatgaaaa atttaggtgt
    16441 agatgtaaac tactatcgtc caccatgggg tcatactaat atattttcaa attcatttgt
    16501 taaaaaatat aatctaaaaa tgactttgtg ggatgtaatg gcagaagatt gggagaaaga
    16561 tagtacagta gacattatta taaacaagtt aatgagtaga acaaaagaaa attctataat
    16621 ttgtttacat gatgcaggtg aaaattcagg tggtgcagta ggtgcacctg agcgtactat
    16681 tgaagcttta aagatagcta ttccaaaact taaagccagt ggtttaaagt tcgtaacacc
    16741 agaaaggatg tagttatgat aagtaaaact gaaaagaaaa ataaatgcct aggtagtgta
    16801 gcttttttag ttttactaat gggcataaca ggatattttg tttttagagg gcagtcagtt
    16861 gaatcattga taaaatcatt aaaaggtgca agccctatgt ttatactaat tggatttgca
    16921 atgatgttta tatatgttgc ctgtgaaggt ataaatatat acttaggtat gaaagcttta
    16981 aaccaaaaga ctactctttt aaaatgtatg ggatatgctt ttattggatt ttattttagt
    17041 tctattacac cttcagcatc aggtggtcaa ccagcacaag tttattatat gaaaaaagac
    17101 gatataaata tatcatattc ttcacttata ctattagtca ttgttgttat tcaccaagta
    17161 gttatactgg catatagtgg aataatgttc attatggaac gtgagtttat attaaataat
    17221 gtaagtggta tgaacatttt actaatatat ggtgtaataa caaatgttgc tcttgtcatt
    17281 ggagtaattg caattatatt ttcaaaaaaa cttgtaaaca attttataat atcaataaca
    17341 aatttattag gtaagttaag aataataaaa gatgttgaaa gttcaagaaa agttataata
    17401 tctcaaattg aagagtatgt taaaggtgct caatatataa agcagaatcc aaaacttgta
    17461 gtccaaattt tagtcataac tattgtacaa ataacagcta tgtttttagt tcctttcttt
    17521 gtatacaaag cttttcatct atcaacatat actgtatttg agatattagc gatacaatcc
    17581 ttgcttaata ttgctgtttc ttcattacca ttgccagggg ctgttggagc ttctgaaaat
    17641 agttttatga cattatttaa gatatttttc cctggtcact tattagttcc ggcaatgtta
    17701 cttagtaggg gaataagttt ttatgcattt gtagctataa gtggattgat atgtatagta
    17761 gtacatgtaa aatcttctga aaaagtaaaa gcagtgaaaa aagttgctta tgtaagataa
    17821 tattatatag attattttta ttaatatgtc aatttgtgtg tatattttaa ttaagcttta
    17881 aacatattta caggagtaga aactattaaa acgtgtgaca ttgctaagct tttgctaata
    17941 aagtataata ttttatatag tttgaaatat ttaagaaata tagcaaaaaa aggagcttgg
    18001 tgatgaataa agagattcca aaaaaaattt tagtagctga tgataatcct gagattaggg
    18061 aaatcgtaga aatactttta actggtgaag gttatgaagt tattatggca acgaatggag
    18121 aagaagctgt aaacttagta gatagtacga ttgatttgat tattttggat gttgtaatgc
    18181 ctgtaaaaac aggatttgtt gcatgtaatg aaattagaga aaaaacgaca gcaccaatat
    18241 tatttttaac tgcaaaaact caagactctg ataaagttat tggattttca gcaggaggag
    18301 atgactattt atcaaagcct ttttcatatt ctgaactaat ttcaagagtt aagtctctac
    18361 ttagacgtta ttatatatac cagggaaagg aaaaggaaag agagccaaat atattaaaaa
    18421 caaaagagtt atgtgtcaat ttagatactc aagatgttac actaaatgga gaacctgtgt
    18481 ttttgacaac tatagaatat agtatattaa ttcttatgtt gaaaaataga aaaaaagttt
    18541 tttcgtctga aaatttatat gaaactattt gggatgagca gtatttttat acagcaaata
    18601 atacaataat ggttcatatt agaaatttaa gaaaaaagtt agaaaaagac caaaaaaatc
    18661 cacaatatat aaaaaccgca tggggaaagg ggtattatat tgattaacaa aattaagcca
    18721 tctaagttgg gagttatact attgatatat gtagtaatct cagctgcagt ggctttcatt
    18781 ttaaatatac cattttactc tgctgttgaa tctatagtaa aagatgtatc agaagggaaa
    18841 gtatatttta aaagagaagt aaatcaactt atggataatt ttcaaactta tgtgaataac
    18901 aatggtataa gagcagatga tggagatgat atagcaaaat ggaatagtga taattggttt
    18961 gtttctatga agatatataa aggaaataag gttttttatg atacattata ctatcctaat
    19021 gaagtacctg ataagagtga ggaaaatgag ttttatagca gtcccatggc tagaaactat
    19081 gagatacaat tttctgatat taaagctaat atagttgtag tagctttatt tagatttaga
    19141 tttcaaaatt ttatagaagc aatttctatt acatttatgg cacttacatt tattataaca
    19201 tttttactgt tgtttagtag aaagataaag tacctaagga aaatagaaat gggtataaag
    19261 atagtagaga gtggcagttt aaagtataga ataccagtaa aaggtaatga tgaattatca
    19321 tcacttgcat ctagtattaa tgagatgagt catacattgg agaatcaact tataaaagaa
    19381 agggagatga agaataaaaa taaagatata gtaacttcaa tatcacatga tgttagaact
    19441 ccattaacgt cagtaatttg ttatttagat ttgattaaag accataaata caatagtcct
    19501 gagcaattgg aacaatacat aaaaaatgta aggtctaaag catatcaaat aaaagattta
    19561 acagatgacc tttttacgca ttttgttaat tctggagagg aagtttcaaa agggtatgaa
    19621 aaaataaatg ctaatgaatt tatttttcaa ttacttttag atagttcata tacattggag
    19681 gagaaaggat ttcatataac aatagaaaat gaactttata accaatatca tatttatgta
    19741 gatgttacac aatttagaag gatatttgat aatttatgtt ctaatataat aaaatatgct
    19801 aggaaagata agcctatagt atttttaatt aaagtagagg attctaaatt actaattata
    19861 caaaaaaata gagtaagtaa tttttctaag aatgtagaaa gttttggaat tggtataaag
    19921 aattgtgaga aaatagttgc atatcataat ggaagcataa atgtaaattc agatgataaa
    19981 gaatttgcta ttgaaatatc tattccagca aaagaatcat aaaaatttaa attatatatt
    20041 gtgtaattgc tattatatat tgtgtaattg cttatgtaaa attctatgaa gcaggaagca
    20101 tgtgacttta gttatgggat gttcaatatt gtgtacctct aggcaagtaa caagtttact
    20161 tacaagacca tataatatta ttatcttaaa aaaaggggac ataataatat ggttttgaca
    20221 ggagcacatt atatttacat tctatttatg gtaattatat taattactat gattatgaaa
    20281 aaggatacaa tagtaccatg tatattaggt gtttttttta tggggttgtt ctttgaaaaa
    20341 aacatttttg gtgctataac agcagtgttt aactctttta ttatatcttt aaatgagttg
    20401 ggtccaatta tcttgattat agctattatg gttgcattat ctaaagccct agaagccaat
    20461 aatgctatac agtatatggt gagaccattt tcgagagtta taaaaaattc taatacagca
    20521 ttttttgtaa ctggatttgt tatgttagtt ctttcttggt ttttctggcc aactccagct
    20581 gttgccttag ttggagctgt atttttacct gttgctatga gagctgggct accagcaatt
    20641 ggtgttgcag tagcattaaa tttatttggt catggtcttg ccttgtctac agactttgtt
    20701 attcaaggag ctccaagtat aacagctggt gcagcaggtg ttgcagtatc atatgttata
    20761 aatgatggta tgattttgtt ttgggtgatg ggaatcgtaa ccatatctat ggcattttat
    20821 acattaaaaa gagatattaa caaaggaatg tttagagaag aactaaaagg ttttgaaagt
    20881 gaagaagtag aagagttcaa tggaaaatct aaaatagcaa ctattttagt agctctggga
    20941 tttcttgctg atataattgc tatgtatata tttgacctaa aaggaggaga tgcatcagct
    21001 cttttgggtg gaacagcagt atttttaata ataattataa atgttataaa ttttggcaaa
    21061 gatagtcttg aaaatgtatg tgagaatata attgatggtt ttgtgtttgg gataaaaata
    21121 tttggagcaa ttataccaat agcagcattt ttctatatgg gtgaagttgc accattgaca
    21181 ggtgtatttg gaaaagtatt agcaccaggt tcacaaggtt tattatcaga tataggcata
    21241 gctctttctc aaacaatacc acttaataaa tttgccgtat caggtataga aactgtagtg
    21301 ggagcaataa ctgggcttga tggttctgga ttttctggga tttcattagt tggttcactt
    21361 gcctctgtat ttggaactgc tataaatgca agtgttggtg cactagctgc attaggacaa
    21421 atcagtggta tatgggtagg tggaggctgt ctagttcctt ggggtcttat ttcagcagcg
    21481 gcaatttgtg gagttagtcc aatagaattg gcaaagcgaa actttgtccc agttataacc
    21541 ggattggttg taacaaccat agtagcaatg tttattattt aataaatcga ataaatcttt
    21601 aattgtataa attaaaaata gatgtgtagc tataatttat tataattgtt ttactaattc
    21661 taagccatac atctattttt attgatattt ttattagtat tctttaataa aataatattt
    21721 ttcattatac tatacataac atctaaatat aacatctaaa atactgttat ctatatttaa
    21781 tatcttgcca aattttttga tgttttattg ccctgtccca ccaacatata taagctatca
    21841 caagttttta cctatactat caccaaacaa aaattctaat tttaatttat tattttataa
    21901 cctatattat attaactata ttagtttgta gactttagat gtaattataa atattaatac
    21961 tccaagagtt caaattcata tcctagtttt cttgcatttg atataaaatc atttaaaatt
    22021 tctgtactag ttttggaaac tgcatgtagt aacaagatag aaccatcatg tagattatcc
    22081 attatttttt gcgttgcttc ttcatgtgat ggttgtttat cagtatccca gtctctgtat
    22141 gcaaaactcc aaaatacagt cttgtatcct aaatttttag tcattgccaa gcttttttct
    22201 gagtattttc ccataggagg tctaaaaaat ttaaccatat ctttcccagt tacatcttta
    22261 tataactttt caacatcata taattcgtca ttaaagtttt ttagattgct tgtttttgtt
    22321 ggcatagatg gatgggtttt actatgattt ccaactatat gtccctcaga aaccattctt
    22381 tttactaaat ctttattttc ttttatatat ggagctgtaa caaaaaaaac tgctttaacc
    22441 tgattttgtt ttagagtgtc gagtattttt gtagtgtatc cattttcata accttcatca
    22501 aatgtaagat atagtgtttt ttgaccatct tttgtaggtc cattgtatag ggcatcatag
    22561 tcagaaaatt taaattcaat gtccgtattt acttctggtg ttttatgctt attgtttggt
    22621 atataaaacc aatcaagagt agtgttatct aaagaagata catctaattt attattatca
    22681 ttattagtag tggcttttga tggtttatct gttccgtctg aaactattac attggaagta
    22741 tctttaccac tatctatttt ttccttatct tcttgtaact gtgtttcctt attttgattt
    22801 tcgttttgat tgttttgtga atttgaacat ccaacaataa aaaaaataga aatagaaagt
    22861 agtgttaaat aatgcaatac ttttctaaac attttgaagc tcctttctaa attaataata
    22921 attgcatata ataataatta tatgattaat tacaattgca tatagtatta ttataattgg
    22981 aaattactat taataatagt tatatcttta aaaataaggt cacaatagta tgacaaaggg
    23041 tgaataatca ataaatttaa aaaaaaatga aaaaaatagt tgaccatatt atgagaagat
    23101 gttattataa taaatgtgct caacaagaag taacaaaata acaagcacaa agaagttaat
    23161 tgaat
//
LOCUS       DAYAOX010000044        22527 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.044, whole genome shotgun sequence.
ACCESSION   DAYAOX010000044 DAYAOX010000000
VERSION     DAYAOX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.044"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            37..501
                     /gene="bcp"
                     /locus_tag="ACGI57_001348"
     CDS             37..501
                     /gene="bcp"
                     /locus_tag="ACGI57_001348"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFG9716429.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            511..1254
                     /gene="yaaA"
                     /locus_tag="ACGI57_001349"
     CDS             511..1254
                     /gene="yaaA"
                     /locus_tag="ACGI57_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFG9716430.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            1352..4039
                     /locus_tag="ACGI57_001350"
     CDS             1352..4039
                     /locus_tag="ACGI57_001350"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFG9716431.1"
                     /translation="MKKRKEWSSKMENHKQMPWHSRATHEILESLDSSEEGLTDTEAE
                     KRLKENGLNELRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIV
                     TVNAVIGIVQEKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAM
                     VPADLRLIETSSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGR
                     GVGVVVATGMDTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGA
                     FYQRPLLPMFMIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGG
                     ATVICCDKTGTLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCN
                     DACINPDKTDEILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVH
                     NIDEKIIAYTKGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGF
                     AKREISSIPKEDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHK
                     VTATTIAHELGIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSL
                     KRIGEVPAMTGDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIK
                     EGRRVYRNIQKVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALA
                     LGVDPPSRNIMKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQ
                     TMAFCVLAFSQMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRN
                     AFNLTVLNNLEWLVTIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      4093..4195
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4305..5570
                     /locus_tag="ACGI57_001351"
     CDS             4305..5570
                     /locus_tag="ACGI57_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HFG9716432.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            5600..6490
                     /locus_tag="ACGI57_001352"
     CDS             5600..6490
                     /locus_tag="ACGI57_001352"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFG9716433.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            6981..7424
                     /locus_tag="ACGI57_001353"
     CDS             6981..7424
                     /locus_tag="ACGI57_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9716434.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            7455..9272
                     /gene="ftsH"
                     /locus_tag="ACGI57_001354"
     CDS             7455..9272
                     /gene="ftsH"
                     /locus_tag="ACGI57_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFG9716435.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            9450..12152
                     /locus_tag="ACGI57_001355"
     CDS             9450..12152
                     /locus_tag="ACGI57_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9716436.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            12186..12884
                     /locus_tag="ACGI57_001356"
     CDS             12186..12884
                     /locus_tag="ACGI57_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9716437.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            13241..14197
                     /locus_tag="ACGI57_001357"
     CDS             13241..14197
                     /locus_tag="ACGI57_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HFG9716438.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
     gene            14374..14529
                     /locus_tag="ACGI57_001358"
     CDS             14374..14529
                     /locus_tag="ACGI57_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716439.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            14659..15672
                     /gene="aroF"
                     /locus_tag="ACGI57_001359"
     CDS             14659..15672
                     /gene="aroF"
                     /locus_tag="ACGI57_001359"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFG9716440.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            15694..16758
                     /gene="aroB"
                     /locus_tag="ACGI57_001360"
     CDS             15694..16758
                     /gene="aroB"
                     /locus_tag="ACGI57_001360"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFG9716441.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            16758..18071
                     /gene="aroA"
                     /locus_tag="ACGI57_001361"
     CDS             16758..18071
                     /gene="aroA"
                     /locus_tag="ACGI57_001361"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFG9716442.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            18049..19128
                     /gene="aroC"
                     /locus_tag="ACGI57_001362"
     CDS             18049..19128
                     /gene="aroC"
                     /locus_tag="ACGI57_001362"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFG9716443.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            19131..20327
                     /gene="pheA"
                     /locus_tag="ACGI57_001363"
     CDS             19131..20327
                     /gene="pheA"
                     /locus_tag="ACGI57_001363"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFG9716444.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFSGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSKFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            20374..21183
                     /gene="aroE"
                     /locus_tag="ACGI57_001364"
     CDS             20374..21183
                     /gene="aroE"
                     /locus_tag="ACGI57_001364"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFG9716445.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            21195..21728
                     /locus_tag="ACGI57_001365"
     CDS             21195..21728
                     /locus_tag="ACGI57_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFG9716446.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            21749..>22527
                     /locus_tag="ACGI57_001366"
     CDS             21749..>22527
                     /locus_tag="ACGI57_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFG9716447.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNND"
BASE COUNT         9016 a         2419 c         4058 g         7034 t
ORIGIN      
        1 ataaaattat taataaaaac aaaaggagag atatttatgt tgagtatagg aacaaaagca
       61 ccagaattta cactagaaga taaagatgga aataaggtaa gtatgtcaga ttttaaaggc
      121 aaaaaagtag tagtatattt ttatcctaaa gataatacac caggatgtac acgtcaagct
      181 tgtgctttta gaaatgcata tgatggattt aaaaaggaag atatacaagt tattgggata
      241 agtaaagata gtataaaatc acatcaaaaa tttgcagaaa agcatgaact tccttttatt
      301 ttactttctg accctgactt agttgctata aaagcttttg atgtatggaa agagaaaaaa
      361 atgtatggaa aaactgcatt gggagttgta cgtgcgacat atattataga tgaaaatgga
      421 attattgaaa aagtctttga aaaagcaaaa ccagacacaa atgcacaaga gattcttgaa
      481 tatttggaaa aacaggagta ataagaattt atgattataa tgttatcacc agctaaaaat
      541 atgaagaata tagaagtatt tgatagagat ttgagtttgc cttgctttat agataacaca
      601 aaagaaatag tggaaaatat aaagacattt ggtatagaag attttaaaaa taaaatgaaa
      661 attaatgaaa aattagctgt attaaataaa aatagatttg aaagcattaa atttgataga
      721 ttaggaaacc ctgcaatctt aacttatgat ggaatacaat ataaaaatat agaagcagaa
      781 aattttacaa ggaaagatga agagtttgca aatagctgta ttcgaatcat aagtggattg
      841 tatggtgttg taaaaccata tgatagtata tatgaatata gattagaaat gcagacaaaa
      901 ttacaagtag gtgagtttaa gaatttatac gaatattggg gaaatcgtat atataaagaa
      961 ctaatcaaag aaaaaacagc tatcgttaat ctttcatcaa atgaatacag caaatccata
     1021 gaaaaattta ttaaagatag tgatacatat attacatgta cttttaaagt aaataaaaat
     1081 ggcatattga aagtggaatc tacacaagca aagaaagcta gaggtatgat gacaaaatac
     1141 attgtaaaaa ataggataag agatattgaa gaactgaaaa agtttgattt agaaggttat
     1201 aagtataaag aaaacttatc taacaatagt gaatacatat ttgtaaaaga gtagaagttt
     1261 tgttaaatat taaatggcat tgttaaattt aggtaaaata tacttagtgt gtattgaatt
     1321 gaacttgatt ttaaaatata attattctta aattaaaaaa agaaaggagt ggtcttccaa
     1381 aatggaaaat cacaaacaga tgccttggca ttcaagagca acgcatgaaa ttttagaaag
     1441 tctagattca tcagaggagg ggctcaccga taccgaggcg gaaaaacggc ttaaagagaa
     1501 tggcttaaat gaacttagaa aaaaaccacc tagaacaact ttacaaatgc tttggagtca
     1561 aattacagat gctatggtta tgatactaat aggagcagca atattatctt tgatatttgg
     1621 cgaatttaca gaagcttttg taatactaat aattgtaaca gtaaatgcag ttattggaat
     1681 tgttcaagag aaaaaagcag aatcatcatt agaagcatta aaaaatatga attcacctac
     1741 agctcgtgtt atgcgtgatg gtgaagaaaa tgttattcca gcaagtaatc ttgtagtagg
     1801 agatattgtt tttttagaag atggagctat ggttccagca gaccttcgtt taatagaaac
     1861 atcaagttta aaaattcaag aggcttcttt aacaggagaa tctgttccat cagaaaaaga
     1921 tgcagataca atcttaccta aagaatgtgt attaggagac cgtagcaata tggcgtatac
     1981 atcttcaata gtcacatatg gtagaggtgt aggtgttgta gtagcaactg gtatggatac
     2041 agaagtgggt aacatagcac atcttttaga ttcacaagat gattttgata ctcctataaa
     2101 acgtaaactt aatactgttg gaaagacatt aagtgtcata ggtattattg tatgcgttgt
     2161 tatatttgca ataggtgcat tttatcagag acctctcttg ccaatgttta tgattgcaat
     2221 ttcccttgcc atatcaatta ttccagaggg attacctgca acagctacta ttgtaatggc
     2281 tttaggcgta cagcgtatgg ctaaaagaaa tgctttaatt agaaaattac ctgctgttga
     2341 aacattagga ggagctacag taatatgttg tgataaaact ggtacattaa ccttaaataa
     2401 aatgactgtt acacatattg ctgtcaatgg agattttgaa tctggaaaag tgacatcaat
     2461 agatactgca tctgaaaaac atccattagt gtataaagag attgtttatg ctggtgcact
     2521 ttgtaatgat gcatgtatta atccagataa aacagatgaa atattaggtg acccaacaga
     2581 gggcgcatta atttttatgg ccaaaaaatt tggaatagat caagaagctc ttgaagaaga
     2641 atatccacgt gtatttgaac agccttttga ttcagataga aaatgtatga caactgttca
     2701 taatatagat gaaaagatta ttgcttatac taaaggagca gtagacgaaa ttcttgacct
     2761 atgtactaaa atattaactt caaaaggtga aagaaacatt actgaagtgg ataaaaagaa
     2821 tatacacgaa ttatgtctta acatgtctaa agatgcattg cgtgtacttg gttttgctaa
     2881 gcgtgagatt tcatctatac caaaagaaga tagtgaaaat atagaatatg atttaacatt
     2941 tataggtatg gttggtatga ttgacccacc aagaactgag gtaattgatg cagtaagtac
     3001 ttgtaaagaa gcaggtataa gaaccataat gataacaggt gaccataaag ttactgccac
     3061 aacaatcgct catgagttag gaatatggag tgaagaaaat actgtgatat ctggtgatga
     3121 acttgataat ttgagtgatg atgaactaga tgaagctgtt aaaaatacta cagtatttgc
     3181 tcgtgtatcc ccatttgata aacttagaat tatacaatct ctaaaaagaa ttggagaagt
     3241 accagcaatg actggggatg gtgtaaatga ctctcctgcc ttaaagtctg ctgatattgg
     3301 tatagccatg ggtataagtg gtacagatgt tgcaaaagat tcttctgaca tgattttaat
     3361 ggacgatagt tttacaacaa ttgcttatgc aataaaagaa ggtagacgtg tatatagaaa
     3421 catacaaaaa gttatacaat ttttgcttgt tggtaatata gcagagatac ttacgttgtt
     3481 tgtagcaata ttattaaatt gggatactcc gcttcttgct gttcatattc tatgggtaaa
     3541 ccttgcaact gctacgcttc cagcccttgc tcttggagtt gacccaccaa gcagaaatat
     3601 aatgaagcac cagccagtga aatcaaccac tttgtttgaa aaaaatctta tacaaagagt
     3661 tgttacccaa ggtatatttg tggcaataat gactatttta gcatataaaa ttggtcttaa
     3721 tatagaaaat cattctgtag gtcaaacaat ggcattttgt gtattagcat tttcacagat
     3781 gttacgagca tttagtcagc gttcaaatac agattcaatg tttaatagaa ataatggtaa
     3841 aaatccattt cttttaatct catttttaac ttctgcattt ttaatagcag taatactatt
     3901 tgtacctgtt tttagaaatg cttttaactt aacagtttta aataacttag agtggcttgt
     3961 aacgattgtt ttagccataa tgtctgtaat tcaagtagag gttgggaaaa ttattaaaag
     4021 aaaaaaatta aaaaaatagt tgacaaaatt aggaaaaata tataagatat aaaacataga
     4081 aaaattaaat aaatcttatc cagagaggtg gagggactgg ccctatgaaa cccggcaacc
     4141 agtatattta tatacattgg tgctaaatcc tgcggcgaaa gctgagagat gagtataatt
     4201 aagcttatac agagcctgag ttatatactc aggctttatt tattttaact ctcatctggt
     4261 aaatagttta atttttttta ctaaaatcta agggggatac aagaatgtat aataaagaaa
     4321 caatatgtgt gcaaggaaat tataaaccag gtaatggaga accaagagta ctacctttat
     4381 atcaaagtac aacttttaaa tatagcagta tagaccaact tgctgaatta tttgatttaa
     4441 aagttgatgg acatatatat tcaagaataa gcaatcctac tattcaagct tttgaagaaa
     4501 aaataagttt actagagggt ggagtatctt ctgtagctgt atcatcaggg caatctgcaa
     4561 atatgttggc agttttaaat atatgtaaat caggagatag tatactttgt tcttcaaaag
     4621 tatatggagg aacattcaat ttactaggac ctagtcttaa aaaatttggt atagatttaa
     4681 tatcgtttga cttagattca agtgaagatg agatagtaga acttgcaaag gaaaatacta
     4741 aggttgtgtt tgcagaaaca cttgcaaatc caactcttga agtcatagat tttgaaaaaa
     4801 tagcaaatgt agctaagaga attaatgttc catttattgt tgataattca ttagcatctc
     4861 cagtgctttg taacccttta aagtatggag caaatatagt tactcattct accacaaaat
     4921 atttagatgg gcatgcttca agtgttggag gaattatagt ggatggtgga aactttaact
     4981 gggataatgg aaaatttcca gaattagttg agccagaccc aacatatcat ggtataagct
     5041 atactcaaaa atttggaaat gccgcatatg caactaaagc aagagttcag ttgcttagag
     5101 actatggaaa ttgtttaagc ccattcaatg cgtatcttac taatttaaat gttgaaacac
     5161 tacatcttag aatggagaga catagtgaaa atgcacttaa aatagctaga tttttagaaa
     5221 aacatgaaaa tgtagattgg attaattacc caggacttga agataacaag tattatgaga
     5281 atgccaaaaa gtatttatca agaggatgta gtggtgtttt atcatttgga gtaagaggtg
     5341 ggttagaaaa tgccaaaaaa tttgtggaaa aattacagat agcatctttg gttacacatg
     5401 tttcagatgt aagaacttgt gttatacatc cagcttcaac tactcataga caattaacag
     5461 aagaacaatt aattgcatct ggagtattgc cttcactaat aagattatct gttggaatag
     5521 aaaatgtaga ggatttaata gctgatttaa atcaagcttt aaatttctaa tattctaaga
     5581 gaaagagaag tgaaaagata tggctttaat actaccaaag ggattacctg ttattaataa
     5641 attattagat gaaggaatag atgtaattta taaagaagat tttaaaaagg aattaaagta
     5701 tgaagaaaac atagacacta agatagctat actaaatcta atgcctataa agatagatac
     5761 agaattagat ttactaagaa ggatagataa aacaggattc aacgtgtctg tggaatttat
     5821 taaaatttca acaagagaaa gtaaaagaag ttgtaatgaa tatgttaaag aattttataa
     5881 aacatttgat gaagccaaag gagagtattt tgatggcttt ataataacag gagctccagt
     5941 tgaacaaatg gagtttgaag aagttgatta ttggaatgaa ctagaagaaa ttatggatta
     6001 ttctaagaga aaaactaaat ctacattata tatatgttgg gcagctcaag caagtcttta
     6061 caaatactac aatgtaaaaa aacttccact aagtcaaaaa tgttttggag tatttaagca
     6121 taaggtagat aaagatagta aaatagtaga tggatttgaa aatgaatttt ttgctccaca
     6181 ttcaagacat actactgtaa atattgaagc tttgaaagag aataaagagt tgagcattgt
     6241 gagtcattct aaagaagctg gtccctatat aattacaaat tcaagagatg tgttcgtaat
     6301 gggtcatagc gagtatgaca aatacactct tgataaagaa tataagaggg atataaataa
     6361 aggagataaa atatctatac ctcaaaatta ctatattaat gatgaccctt cagaagagcc
     6421 aactgtaaaa tggaaaaaac attcagaatt gttgtttaga aattggataa aaaactattt
     6481 gatacaataa attttaagag tatataaatg ttaactaaaa aaatatagaa atttataggt
     6541 ataaagttaa tcttaatgat actttatacc ttattttttt aaaatttgac cttataatag
     6601 tagttttata ttttaagaaa atcactttag gtgtatgtag atagaggtgg ctttaatttt
     6661 atatatggtg cgataatggg agactaaacc tttatagtat caataaattt gtattttaac
     6721 ttttaaaaga gattaaaaag ggagaaatca gtgtaaaata tatatataga ttttttatta
     6781 aattttatta aaaatatcct gtataaaatt aaatatcaat aagataacaa aatttcaatt
     6841 gaagttacaa tattgtaatt gacaataaat acttaatact ataatattta atacattaaa
     6901 cattatagtg ttaagtattt atttacagta tgcacttcag cctaaatttg aaataaagta
     6961 ttttcgtagg gagcgtttgt atggatgaga tgagttttaa gaaagagcta ctagagttga
     7021 ctagagatat aaatatgaaa tttacaactt tattaagtga tttttatcag ccacttggta
     7081 ttacagcagt gcaagctttg attttatctg agttatgtga acatggagag aaaaaaataa
     7141 gcgatttagg gaagaactta aacatgacaa atagtaatgt atcagtaatc tgtcaaaggc
     7201 ttgaaaaaaa tggcttttta aatcgcataa gagatataga agaccaacga attgtaaaag
     7261 taaaagttac aaataaatca ttggatatac aagaacatat ttcaagtagt atttttgata
     7321 gttattttga aaatatgacc agtgaaaaat tgcaagatat ggaagatata atagaaggtt
     7381 tagagaaatt aaataaactt ttaacttaca ttgattgcaa ataacataaa aataatttaa
     7441 aggagaataa aattatggat gaacataaac cccaaaaaag gtcagcactc tattattata
     7501 cagttataat aatagtagta atgtttttaa atgctttaat atttccaagt ttaatggata
     7561 aaaaaatggt tgagattgac tatggaaagt ttctcacaat gttggataca ggaaaggtta
     7621 aatctgttga gatagaaaat aatagaatag caataaatcc atctagtagc aaggataaga
     7681 atatatacat aactggtaga atggatgacc cagaattggt agatagacta aaaaatgcaa
     7741 aagtagaatt tactaaggtt attcctaagg aaaattcacc tcttttaagt attttactta
     7801 catggataat acctataggt attcttatgc tatttggtaa cttaatgatg aagtctatgc
     7861 aaaagcgaat gggtggtaat gctatgcaat ttggaaaaag caacgccaaa atgtatgtat
     7921 ctgctcaaag tggaaaaaga tttaatgatg tagctggtca agatgaggca aaagaagctt
     7981 taactgaaat agtagatttc ttacacaatc cagaaaagta taaaaagata ggagcacaaa
     8041 tgcctaaagg agcactttta gtaggacctc caggtacagg aaaaacactc cttgcaaaag
     8101 ctgtagcagg tgaagcaaat gtaccatttt tctcaatatc tggttctgag tttgttgaga
     8161 tgtttgtagg aatgggagct tctaggattc gtgatttatt taaacaagca aaagaaaaag
     8221 ctccatgtat agtgtttata gatgagattg atactattgg taaaaagcgt gataatggaa
     8281 gtggattagg tggaaatgat gaacgtgaac aaactttaaa tcagttgtta agtgagatgg
     8341 atggttttga tggtggaatt ggagtagtaa tacttgctgc tacaaataga cctgacagtc
     8401 ttgacaaagc attacttcgt ccaggaagat ttgatagacg tataccagtt gaacttccag
     8461 accttggagg acgtgaagct atattaaagg tacatgctca aaatgtaaat gtaagctcag
     8521 atatagatta taatgcaatt gcaaggtcta catcaggagc atcaggtgca gaacttgcaa
     8581 atattgtaaa cgaagcagca cttttagcag ttaagtctgg cagagatata gtagaacaac
     8641 aggattttga tgaatctgtt gaaactgtaa ttgctgggta tcaacgtaaa ggagctgtaa
     8701 tttcagataa ggaaaagaaa attatagcat atcatgaaat aggtcatgca cttgttgctg
     8761 ctatgcaaaa acattctgca cctgtccata agattactat aatacctaga acttcaggag
     8821 cattgggata tactatgcaa gttgctgaag atgagagtgt acttatgtct aaagaagaag
     8881 caattgataa aataactact tttacaggtg gtcgtgctgc tgaagaggtt atctttaaca
     8941 cttgtacatc aggagcatca aatgatatag aacaagctac taaaattgca agagctatgg
     9001 ttactcgcct tggtatgagt gaagagtttg atatggtcgc tcttgaaact gtaagcaatc
     9061 aatatttagg aggagatact tctcttgctt gttcttccga cactgctgct aaaatagatg
     9121 aagaggtatt taaaatagta aaggcttgtc acaaaaatgc aattgaaatc ttagaaaata
     9181 atattgataa attgcatgaa cttgctaatt atttattaga aaaagaaact attacaggtg
     9241 atgagtttat ggaaatactt aacttaaact aaaatgatta tacacaagat aaaaatgcct
     9301 attgttaggc atttttattt tacaactaaa ttttttaaat ttatatttaa atctttacat
     9361 aatcttaatc attaaaaata catcttaact acaattttat atcggaattg atattataat
     9421 agtaatgaat ggtgtaagga ggttttttaa tggatagacc taatcctgat attctattag
     9481 aaaaaattaa aaatgaagaa gaaaaactaa gtagaggtca attaaaaata ttctttggtt
     9541 atgcagctgg agtaggaaaa acctactcta tgttggaatc tgcacaaaat ttaaaaaaag
     9601 tgggtgtaga tgtagtagtt ggatatattg aaccacacac tagaccagaa acattggctt
     9661 tacttgatgg cttggaaatt cttcctgtaa aggagataga atataaatct ataaaactaa
     9721 aagaatttga tttagatgct gctttagcaa gaaaaccaga agttatatta gtagatgaat
     9781 ttgcccattc taatgttgct gggttaaggc atacaaaaag atggcaagat atagaagaat
     9841 tgctgttagc aggcataaat gtctacacta ctgtaaatgt acaacattta gaaagtctaa
     9901 atgacattgt tgagataatt acaaacgtat cagttaaaga gactatacca gataagtttt
     9961 tggatacaaa tacacagtta gaacttatag atgttgaacc tgatgtactt cttgaaaggt
    10021 tcaatgaagg taagatatat aagaaagaac aagctactag agcaaaaaat aattttttta
    10081 taaaagataa cctggttgca cttagagaaa ttgcacttag aaaaacagca gagagagtta
    10141 ataaagaagt tcaaatgacc agactatcaa agggtgatgt aactgtaata cctacatcag
    10201 atacacttct tgcatgtata agtccatctc catcttcagc aaaagttatt agaactgcat
    10261 ccagaatatc agacagtaca tttgcaaaat ggatagcttt atatgttgaa actccaaaca
    10321 cagcaagact tccatttgat gaacaaaaac aactacaaaa taacctaaaa ttagctaaga
    10381 aattaggtgg agaaataatt gttttacatg gtgaaaatat aatagagcaa atacttagaa
    10441 ttgcaaagct gagaaatgta acaaaaattg taataggtag aaatcattca agtaataaaa
    10501 gatttagtaa aagatttaaa aaagatatag ttgacaaatt aattgatgaa gttgattata
    10561 tagatataca tataattcca tataaatttg cctcagatgt taaatacaaa cctaaaaagg
    10621 ataaatcatc aataaaatct aaatttaaaa ttagcaaagt tgattttata aaacttatat
    10681 ttattacttt gatgataagt attttagcat atgcagtaca aagcatggga tttataagag
    10741 aaaatatatt gttaatatat atgttgggtg tagttttagt ttctatgtgg actaaagggt
    10801 atagtacagg tataataagt tctgtattta atatagttct tttaaattat ttttttacag
    10861 caccattata tacattatct atagcagatt caaactatat agtaacactg gttgtattct
    10921 caatagtagg tataataact agcactctta cgtctaagat acagcatgaa gcagaaactg
    10981 ctgctaaaag agaagaaaat acaaaaatga tatatcaaat aattaaggga tttttaagat
    11041 tatcaaataa agatgatata gtaaacaaag gtatagaact tttgtctctt agcttaagta
    11101 gagatgtaat ttgttattta aatgtagata agcataataa atcaaagctt tacaaaaaaa
    11161 atactaataa taaggataaa aatgatttgg atagtgaaga tgaaaaagct gttgcaagtt
    11221 gggttttatc caattcaaca gtagcaggaa atgacacgga tacacttcct ggttcaaagg
    11281 gatactatat accaattgta ggaatgaatt taactttagg agtagtggga atatcatgta
    11341 ttgattcaaa attggataca gaagatataa gcttaattga aacaataatt gctcaaatgg
    11401 caatagcgct tgatagagaa atcttgtcag aagctaaaga aaataccaat cttgagattg
    11461 aaagggaaag attaagaagt aatcttttaa gagcagtttc gcatgacctt agaactccac
    11521 tagcaggaat atctggtgca gtaagtacaa ttataaaaaa caagggtact ataggtcaag
    11581 atataataga tgaattactt agtggtgtat atgaagatac tcagtggtta ataagacttg
    11641 ttgaaaattt acttagtatg accaagatag atgaagggaa attggaagtt aaaaaacatt
    11701 ctgaactggt tgaggaaatt atatcagaag cgttacaaaa aataaaaaaa agaattgaaa
    11761 atgcacttat agatattaat ataccagaac agatattatt tgttcctatg gatgcaaaat
    11821 taatagaaca agttttaatt aatttaatag ataattcttt gaaatattca aaggaagatt
    11881 gtaagataga gataaatgta tatgagaaag atgattatgt ttggtttgaa gtatctgata
    11941 atggtcctgg tatatcaaag gaacttaaaa agcatatctt tgacagattt tttactggag
    12001 aagaaggagc taaagattcc agaaagggtg ttggacttgg attatcaatt tgtaaatcta
    12061 taattcaagc tcataaaggt gaaattatgg tagaaaacaa taaagataag ggttcaactt
    12121 ttaagtttag tttgcctaag gaaaatgaat agttttaaat tttaaaatta gttttggagg
    12181 aagaaatgag taaaaaactt atattgttag tagaggatga taagacaata agaaaattta
    12241 tatctactgc acttttaaca caggattatg atgtcaagga ggctataact ggaaaagaag
    12301 gtatatctat agctgtctca tattctccag atgttgttct tttagattta ggcttagaag
    12361 atatggatgg aatagaggta ataaaagcta tcagacaatt ttcaaatata ccaataattg
    12421 tagtttcagc tagagagcaa gatagagaca aggtagaagt atttgatgca ggggcagatg
    12481 attacttaac aaaaccattc tccatagtag agttattagc aagagtaaga gtagcattta
    12541 gacattcaca agtagaagct caacaaaaag atgatgttaa atctacgttt gaagtagata
    12601 aattattgat tgattttgat aaaagaaaag ttattgttga tgatgttgaa gtccatttga
    12661 cacctattga atataatatt ctttcattac ttgcaaagta tcatggaaaa gtgcttactc
    12721 ataattttat aataaaagaa atatggggta gtgttatagg gaatgagact aagtctttaa
    12781 gggtctttat ggcaacgtta agacgaaaaa tagaaaaaca acctgcaaac ccaagatata
    12841 taattacaga agttggtgta ggatacagat taaatgatga atagttgtaa tatattaatt
    12901 attagattat gtgcttatta atatatatta aaattatata gtttagttaa aataagaatg
    12961 aagtgtatgt gcttgttgtc agtttagcac atgcatttta ttttttagct tttttagtat
    13021 caataatttt ttagtatcaa taatatgaaa tatgttttaa aataagtata tagtgtaata
    13081 tgtaataaaa actttggtta aataataaat gtatttaaca cttaccttat aataaattta
    13141 atataaatat taaataataa ttattaaaat agtgtaaaat aattacagta aggattataa
    13201 aaaattttta gaatgtaaat tgaaatttag gagtagatgt atgtatatac tgaatttaaa
    13261 tacaagagag actattgaag attttagaga taaattttat gtagcagaaa attcttatct
    13321 tatattaagt gctccaaaaa atttaaaatt gcttaaggaa actttggata tagatgaaat
    13381 aacttttaat gattgtctta aatttgatga aataaccaaa cttgatttat ttgataatta
    13441 cgatttttta agcctaaata catttgaact aagagatgga gaagctgtta tagaagaggt
    13501 aaatatgtat ttatcagata attttatatt ggttgtagtg aatgaagaac actttttatt
    13561 tgaatttgta aaaaatatta ttttgaagaa ctctcaactt gaaaaaaatc cagttattaa
    13621 tctatttaag ataaactact tgattcttag agaagttata aaaaatggtt ttgagtcttt
    13681 ggaaaaagtt gaagagttaa ttcttcaaat agaagatgaa atgatggaca acattaataa
    13741 gaatcatgta tctagaataa gtgatgtaag aggattaaca agaattatag ttaagaatac
    13801 tagacctctt ttgtatattg gggatagaat agttaaggaa aatataagat atttaaaata
    13861 ttcaaatgta aaaaaatata atttagaaaa ttttcaaggt atagattttg gaattgacaa
    13921 attatacaat ttcgcattgt caacacgaga gttagcagat aaattattag atatttattc
    13981 ttccagagta ggtgagaaaa caaataactt aattacaaaa ttaaccttgt taacagccat
    14041 atcagcacca cttacaatta taactggtat atatggaatg aactttagat atatgccaga
    14101 attaaactgg atttatggat atccagcaac tttatttttt atgctatgta taatttttgt
    14161 tggaattata atatttaaaa taaaaaaatt attgtaaatt acagatgatg tttaattgat
    14221 tttataaaaa tacatcaatt gagaagctga tttaagtaaa taaaaaaata ttttaaattg
    14281 aaaatattta tagaaaatat tgacaattat attttctttg ttataattat taaacaagaa
    14341 gaataataaa ttataaaaat ggaggataat actatgaaag gaatattaaa agaactaaca
    14401 gcaaaacttt atagcttttt ttactttatg ttctggggct tttattttag cgctggctat
    14461 ttcttttaca aaaatatatg tacttccttt ctggtgaggc cttattttag tgattttgag
    14521 cataagtaaa ttttataaaa atgattattt tatatattta tataagattt agtgcttgat
    14581 agattatttt agagaactca ttagtgagtt ctctttttta ttttaaatat aaaattataa
    14641 tattgggagg tagtaaaaat gatagtagta ttaaaaatgg gagcagataa aaatgaagtt
    14701 aaaaaattaa ttgaggctat tgggagagaa ggagtagaag ttaatcccat agatggaaca
    14761 gaattaactg ttttaggttt agttggagat actagtaaaa tagatgccaa aagaatagaa
    14821 gcaaataagg tagttgaaaa agttatgcat gtggtagagc cttttaaaaa ggccaataga
    14881 aaatttcatc cagaaccttc aatcataaat gttaatggaa tggaaattgg ttcaaagaaa
    14941 attgctatga tagctgggcc ttgttctgtt gaaacagaag accaaatagt atctatagct
    15001 aaagatgtga aaaaatcagg tgcaggattt ttgagaggtg gagcattcaa accaagaaca
    15061 tcaccatacg ctttccaagg attgaaatat gatggattgg atttattaaa aaaagcaaaa
    15121 gaaaaaacag ggcttcctat agttacagaa ataatgtcaa cacaagatat agatatattt
    15181 gaagaaaatg tagatgtaat tcaagttgga gctagaaaca tgcaaaactt tgatttatta
    15241 aaagaattag gtaaaacaaa taaaacaata cttttaaaaa gaggattatc tgctacaatt
    15301 gaagagtggc taatgtcagc agagtatata atggctggag gaaatgaaaa tgtagttctt
    15361 tgtgaaagag gtataagaac ttttgaaaca tatactagaa atactttgga tttaagtgca
    15421 attttagctg tgaagaaact tagtcattta ccagttatag ttgacccaag tcatgcagca
    15481 ggtaaatcat ggatggttga ctctttatct aaggcagcaa ttgcagttgg agcagatggt
    15541 ctaataatag aagttcataa tgacccagca catgcacttt gtgatggaaa acaatcgatt
    15601 aagccaaatg aatatgatga attgataagt gaattaaaga ctatcgcatc agcagttggt
    15661 agagaaatat aatctataaa aggatggtta aacgtggaag aaaaactatt agtaaattta
    15721 aaagaaaata gctacaatat atttattaaa aaaaatatac taaagaatat aggagaagaa
    15781 ataaaaaaaa tatattttgg aaataagatt tttattgtaa ctgatgaaaa tatagcaaaa
    15841 ttatacttgg ataaagtaaa aaataattta agtgaatctg gatttgaaat taaatcttta
    15901 atattaaaat ctggtgaaga aacaaaatca tttaatactc ttcctaaaat atacgatagt
    15961 ttacttgact ttaagttaac tagaaaagat ttgattatta ctttaggagg aggggtaata
    16021 ggtgatttag gtggatttgc agcagcaact tttttaagag gtataagttt tattcaaatt
    16081 ccaacatctt tattagcaca agttgatagt tctgtaggtg gaaaagttgg agttgacctt
    16141 gagagaggca aaaacttagt gggaagcttt taccaaccaa aggctgtgtt tatcgaccca
    16201 aatgtattat tatcacttcc agaaaaattc tatagagatg gaatggcaga agttataaaa
    16261 tatggatgta taaaagacaa aacttttttc aataaacttt taagtttaaa aaatagagaa
    16321 gaagtaatgc aacatataga atacattata cataaatgtt gctttattaa aaaaactgta
    16381 gttgaagctg atgaaaaaga ctttggagat agaatgcttc taaactttgg ccatacatta
    16441 ggtcatgcta ttgagaaata ctacaatttt acaggttata cccatggaga agctgttgcc
    16501 ataggaatgt ataagataac tttagaaagt gaaaagatag gaattacaaa aaaaggtaca
    16561 tcagaactta taaaatcaat tttaatgaat tataatttgc cttatgagat tgaattaaga
    16621 gataataaat ctatagtaga tgctatatct cttgataaaa aaaatctagg tgatacatta
    16681 aaaataatac ttttaaaaga tattggagag agtattatat ataatactac ttctaaattt
    16741 tttcaggagg taagataatg ggaagtctaa aaatatatcc aagtaaatta tctggagatg
    16801 taaaaatacc accatcaaaa agtatggcac atagggcagt tatttgttca tctctaagca
    16861 atggaaaaag tagaatttct aatatagatt tttctgatga tataatagct actataagag
    16921 caatgacatc attaggagct attatagaaa agaaagaaga tattttagaa atttcgggaa
    16981 ttttttctaa agaaggtatt ttaaatagag agaatcagtt gaatcaacct aaattgacaa
    17041 tagattgtaa tgaatcaggc tctacattga gatttttagt acctatatct ttaacatttg
    17101 atggtgtaaa aaggtttata ggtagaggta atcttggaaa aagaccactt gatacctatt
    17161 atgagatatt tgacagacag aatataaaat atagttacaa agaaaatcag ttagatttga
    17221 ttatatcagg taagcttaaa ccagatgaat ttagagtaaa aggaaatata agttctcagt
    17281 ttatcacagg tttactgttt atattgccta cgctagaaag tgattcaaag ataataatta
    17341 ctactgagct agaatctaaa ggatatttag atttaactct ttcaacgata aaagattttg
    17401 gggtagaaat tataaacaat aactataaag aatttattat aaaaggaaat caaacgtata
    17461 aagcgagaga ttataaagta gaaggagatt attctcaagg agcattttat ttatcagcag
    17521 atgctatagg agaagatatt agcatattag accttaaaga agattctctt caaggggata
    17581 gtgaagtagt tgaaatttta tctaggatgg gaatggaaat attaagagaa ggcaacaaaa
    17641 taaaaggaat aacaaatggt ttaaatggta ctttgataga tgcatctcaa tgcccagata
    17701 taatacctgt attatctgta gtagcatcct taagtatagg gaggactaca ataattaatg
    17761 ctggtaggct tagaattaaa gaatgtgata gacttcatgc aataaatgtt gagttaagta
    17821 aacttggagc aaatatagag gagaaagagg atagtttgat aatcgaagga gtaagcaagt
    17881 taaatggtgg agtagaggtg tggagtcaca aagaccatag aattgctatg acacttgcaa
    17941 ttgcttcttg tagatgtgat aaaccaataa tattaaaaga ttttgagtgt gtatcaaaat
    18001 cttatccaca tttttttaaa gattttaaga tgttaggagg tcgaattgat gagtggaata
    18061 tggggaaata atttaaaagt atcaattttt ggagaatctc atggaaatgc tataggtata
    18121 aatatagatg gtcttccttc ggggattgaa cttgaccttg ataatattga taaagaaatg
    18181 aagagaagag cacctggaaa aaattctata tctacttcaa gaaatgaaag tgatatacca
    18241 gaaattctaa gtggatattt taatggaaga actacaggaa cgccattatg tgcaatcata
    18301 agaaatagtg atactcgttc aaaagattat ggagagctta aaaatttaat gagaccagga
    18361 catgcagatt tcacaggtaa tgtgagatat tctggattta atgactatag agggggaggt
    18421 catttttcag gtaggataac agctccatta gtattttgtg gggctatatg taaacaaatt
    18481 ttatcacaaa aaggaataga gataggagca cacataaaaa aaattaaaaa tatagaagat
    18541 atgagttttg attatgtaaa tatttcaaaa caacagttat caaatttgca aactttggaa
    18601 ctacctttac ttgatttatc aaaggaagaa gccatgaaaa atacgattat agatgcaaaa
    18661 aatcaaggag attcagttgg tggtataatt gagtgtgcag tagtaggtat taatgtaggg
    18721 cttggaaatc cattttttga ctctgttgaa tctactttat cacatttatt gttttctgtt
    18781 cctgctgtaa aaggtgtaga gtttggttta ggttttgaac ttgcagatat gtatgggtct
    18841 caaagtaatg atgaaatgta ttatgaagga aatcaagtta agagcaaaac aaataacaat
    18901 ggaggaatta taggagggat tactactgga atgccaataa ttttcaaagt ggcaattaag
    18961 ccaactccat caattagcag acaacaaaac acagttaata taaaagataa aaaggacgat
    19021 attttatata ttaaaggtag acatgaccct tgtatagttc aaagggctat acctgtaata
    19081 gaagcagtaa cagccattgg aatttttgat ttgatgaaag gacgataata atggaggatt
    19141 taacgagata tagaaatgag attgacaata tagataaaga gcttattcaa ttatttgaaa
    19201 agagaatgaa tacagttctt gaaattgcca gacataaaat gaaaaataac actactatac
    19261 ttcaaaaaga tagagaagaa aaagtactaa gtagagctgt agataattta gataataaag
    19321 catattcaca agaaacggta cagtttttta attctataat ggaaattagt agaggattac
    19381 aaaaaagatt gatagataat gatggtgagc agaaaatcga gtttagttta gattcatcaa
    19441 ataagaaatt tgatttgtca aatgtaaata gatataagtc tttaaaagat gaattaaata
    19501 aaaaaaatat cctagtagga ttttcaggaa aatcaggttc atttacagaa gaagcactaa
    19561 ataaattctt caacaaaaaa atatcaaaaa aacaatttaa agaatttgaa gatgttttca
    19621 tagcacttaa aaataaagaa ataaactatg gaataatccc catagaaaat tcatctacag
    19681 gagctatctc tgaaacttat gaccttttga gaaagtacgg tttttatata gttggagaag
    19741 aatgtataaa aatagaccaa aatttaatag ggataaaaga cacaaagctt gaagatataa
    19801 aagaaatata ttctcatcca caaggtatag gacaatgtag taaattttta aaacaaaata
    19861 gtacatggaa attgatacca tttaataaca cagcaacaag tgcagaactt gtaagaaaac
    19921 ttcaagataa aacaaaagca gcaattgcaa gcaaaaaagc tgcaagtatt tatggattag
    19981 aaataataag tccttgtata aatgatattg caaacaacta tacaagattt gtagtaatca
    20041 gtaatcaaat acacattgaa gaagaatcaa acaaaatgag tgtagtattt tctgttgaac
    20101 atgaagcagg aaaactttat aaagtacttg gatactttgc taaaaataat ataaatatga
    20161 caaaaataga atcaagacct atgaaaaatg cctcatggag atatttcttt tatatagatt
    20221 ttgagtgttc tatatacaat gctaaagtgt acgatttatt agaattgata gaacataata
    20281 cagcatattt taaatttatg ggagtatata aaaataaaac tgaatagata agataaattt
    20341 ttatttaatt tataattatt aggaggtgta agtatgaatt ttttcggtct agtaggtgaa
    20401 aaactctctc acagtgtttc acctcaaatc cacaagagag tattcgagat attgaatatt
    20461 gaaagtgctt ataaaaactt tgaaatttca aaagaagata tatctaagct agacggtgct
    20521 ataaaattat taggaataca aggggtaaat gttactgtac catataagga gcgtattatg
    20581 aaatatcttg attttatatc accagaggca aaacgtatag gagcagttaa tacaatttta
    20641 cttagagaaa atatgttata tggttacaat acagattact ttggtcttga tagtatgttt
    20701 aaaatggcta atattgatgt tcaaggaaaa gtagcagtta tattaggaac tggaggtgct
    20761 tctaaagctg ctcttacata ttttattgat tctggtatag aaaagcttta tgtttcaact
    20821 agaaaaaagg atgataaaaa attattaaat agtaaagcta tattaataga ttatgaagaa
    20881 cttaaacata taaaaggaga tataatatta aatgctacac cagtaggtat gtaccctaat
    20941 gtaggaattt caccagtgtc aaaatctatt atacaaaatt ttgatatatt aatagattta
    21001 atttacaatc ctggagaaac agagttttta agaattggta atagtatggg taaaaaaact
    21061 tgtgatggat tatatatgct ggtggggcaa gcaataaaat ctcaggaaat ttggcaagat
    21121 actaaaatag ataatagtat tttagatgtt atatataatg aactaaaatt agaatttttg
    21181 taagggagat tgttatgata aataaaacaa aagaaaaatt gatattaata ggtatgccag
    21241 gtagtggaaa aactacaatt ggtaaacttt tggccaaaga atataattgt agtttctgtg
    21301 atatggatga ttatataatt caaatatctc aaaaaagtat agcagagttg ttttcagaag
    21361 gagaagatat ctttaggaac tatgagactc aagcatgtag agaactttct ataagtgata
    21421 aaacagtgat atctacaggt ggaggtgtaa taaaaaagga tgttaatatg gaaatactaa
    21481 aagaaacagg tataataatt tttattgata gacctataca aaagattttg gaagatataa
    21541 atattaattc aagaccatta ttaaaaaacg gaaaagatag attgtataat ctttataatg
    21601 agagaataaa tctttataaa aaattttcag atatagagat tttaaatgat aaatcattaa
    21661 ataatgctgt atataatatt acaaatgcag tcagtgaaaa ttttaaattt gactttaaag
    21721 aaaaataatc tagggggaaa taaaaataat gaatattgtt attgtaggac taggagttat
    21781 aggaggctct tttgctaaag cattaaaaaa agctggatat gaaaatgtat ttggagtaga
    21841 tgtagattta gaaactttaa aaaaagcaga aaaagcaaaa atcataaaaa aaggatgtac
    21901 tacaggtaaa gagttattta aaaaagctga tttaattata ttatctatat atcctagatt
    21961 agttgtagat tttctaaata ataataaaaa cttttttaaa aaagggacta taattactga
    22021 tactacagga ataaaagaaa cacttatcaa tgatgtgttg caaattattc cagatgatat
    22081 agattttata tttggacatc caatggcagg tagggaaaaa aagggtattg attttgcaag
    22141 tgaacaagtt tttaatggcg caaattatat aattactcca actggcagaa acaacataaa
    22201 aaatttagag ttagttgaaa atttaatttt agagattgga tttaaaagag taaaaaagtt
    22261 aacttcacaa aaacatgatg agattatagc ttttacttct cagttacctc atgttatggc
    22321 agttgctctt ataaatagtg atgaagaagg tagagatacg ggtaaattta ttggagatag
    22381 ttatagagat ttaactagaa ttgcaaatat gaatgaagac ttatggagtg aacttttttt
    22441 aggaaataga gataatttac taaaagttat agaaaacttt gaatcagaag taaatttagt
    22501 aaaagaagct atatttaaca atgataa
//
LOCUS       DAYAOX010000045        22221 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.045, whole genome shotgun sequence.
ACCESSION   DAYAOX010000045 DAYAOX010000000
VERSION     DAYAOX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.045"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            213..1034
                     /locus_tag="ACGI57_001367"
     CDS             213..1034
                     /locus_tag="ACGI57_001367"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFG9716448.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    1113..1324
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1411..2727
                     /gene="argH"
                     /locus_tag="ACGI57_001368"
     CDS             1411..2727
                     /gene="argH"
                     /locus_tag="ACGI57_001368"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFG9716449.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            2976..3314
                     /locus_tag="ACGI57_001369"
     CDS             2976..3314
                     /locus_tag="ACGI57_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HFG9716450.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGKP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(3441..4730)
                     /locus_tag="ACGI57_001370"
     CDS             complement(3441..4730)
                     /locus_tag="ACGI57_001370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9716451.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            4810..5514
                     /locus_tag="ACGI57_001371"
     CDS             4810..5514
                     /locus_tag="ACGI57_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFG9716452.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKLNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            5606..6652
                     /gene="selD"
                     /locus_tag="ACGI57_001372"
     CDS             5606..6652
                     /gene="selD"
                     /locus_tag="ACGI57_001372"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5654..5656,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFG9716453.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            6703..8082
                     /gene="selA"
                     /locus_tag="ACGI57_001373"
     CDS             6703..8082
                     /gene="selA"
                     /locus_tag="ACGI57_001373"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFG9716454.1"
                     /translation="MLPSVDEVLSDNRIVEIINEYPRSLVLESVREVIDLKRQFILRL
                     KEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLGRSLLSEDIKDE
                     LWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNAAAVLLVLSTMA
                     KGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYKEAITEDTNVLM
                     KVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKYGLSYEPTVLDS
                     IRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDKLTICALEATLR
                     MYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINIEDGFSQVGGGS
                     MPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDVRTIFDDEFDVI
                     VEELRKAFN"
     gene            8196..10079
                     /gene="selB"
                     /locus_tag="ACGI57_001374"
     CDS             8196..10079
                     /gene="selB"
                     /locus_tag="ACGI57_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFG9716455.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            10184..12922
                     /locus_tag="ACGI57_001375"
     CDS             10184..12922
                     /locus_tag="ACGI57_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HFG9716456.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIITNVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEHELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(13041..13985)
                     /gene="manA"
                     /locus_tag="ACGI57_001376"
     CDS             complement(13041..13985)
                     /gene="manA"
                     /locus_tag="ACGI57_001376"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFG9716457.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            14158..15519
                     /locus_tag="ACGI57_001377"
     CDS             14158..15519
                     /locus_tag="ACGI57_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9716458.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            15607..17496
                     /locus_tag="ACGI57_001378"
     CDS             15607..17496
                     /locus_tag="ACGI57_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9716459.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            17511..17966
                     /locus_tag="ACGI57_001379"
     CDS             17511..17966
                     /locus_tag="ACGI57_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9716460.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            18025..18342
                     /locus_tag="ACGI57_001380"
     CDS             18025..18342
                     /locus_tag="ACGI57_001380"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:NF014368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFG9716461.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            18367..19629
                     /locus_tag="ACGI57_001381"
     CDS             18367..19629
                     /locus_tag="ACGI57_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFG9716462.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            19734..20798
                     /locus_tag="ACGI57_001382"
     CDS             19734..20798
                     /locus_tag="ACGI57_001382"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFG9716463.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YAGNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            20852..22030
                     /locus_tag="ACGI57_001383"
     CDS             20852..22030
                     /locus_tag="ACGI57_001383"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9716464.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         8971 a         2255 c         3926 g         7069 t
ORIGIN      
        1 tttctagcat tataagacta taaattgaaa atattcagaa aaatactaca agaaacgatt
       61 tattttgctc ttaaaataag ttaaatgaat gttattaatt ttgtaaattg aaatagttta
      121 agtataaagt tatgatataa tctaataagg gtaattcttt agattatatt aaggaaatat
      181 aggtatacaa taaaaagtta tggagttgga ttatgggata taaattaatt gtaacagata
      241 tggatggaac tttattaggc aataatcata aagttacaga tgaaaataaa actgcactac
      301 aaaaagttat aaaaagcggt attaatgtaa ctttagcaac aggaagagcc ttcgattcag
      361 ctaaatgcaa tgtagatttt cttaaagagg acatgccaat aatagcttgt aatggttcat
      421 taataagaga gcaaaatgga aatataatat attcaaataa gattgataca agaacatgtt
      481 taaacatttt agatgtatta gataaatacg atatttacta ccaatgtaat agtatagatt
      541 ctatgctttc gaaaaaaata gaaggacgtg aagatagatt aagtgtattt ttaggctctg
      601 aaacagaagt aattgtaaaa gatgatttga gagaagaaat ctttaaaaaa gatatactaa
      661 aatttgtaat catagaagaa aaaaatccat ctattttgga tgaaataaga aaagaattga
      721 gaaaagttca aggaataaag ataacctctt catggccaaa caatattgaa gtcatgaatg
      781 aaggtgttga taaaggtaat gcagttaaaa ttttagctga aaaaatgaat atagacagag
      841 aagatataat tgcatttgga gacaattaca atgacataga aatgataaag tttgctggtc
      901 ttggggtagc tatgggaaat gcagaagaat tgattaaaca agaagctgat tatgttacag
      961 atacaaatca agacagtgga gttgcaaagg caatttataa attcctagag ttaaaagaat
     1021 cacttagctc ttaaaacagt atataatagg aaaaaatagg ttgactattt tgtagatagt
     1081 aattataata caaatagata aaatataaaa atttagattc agtgaaaaag agagtagata
     1141 tagagaaaat ctcagcgaat tgggggatgg tgtgagccta atgataacac tttatattga
     1201 aaagaacttt ggagaatact acttgaaatt cagtatggta gtaggtaatc tcgccttaag
     1261 agaatagagt gggtaaacat tgtttaccaa ctagagtggt aacacggaat atttcgtctc
     1321 ttggtctttt tgaccaagag attttttagt ttcattgagt tacaagactt ataattaaaa
     1381 tacaaaaaat taaaatatga ggtgaaagtt atgaaattat ggggtggacg ctttagaaaa
     1441 gcggaaaatc aacttatgga agaatttaat aaatcttttg gctatgattg tgtactgtac
     1501 aaaaaagata tcgaaggaag tgtagcacat gttcatatgc aagtaaaatg tggactttta
     1561 acagaagaag aaggaaagtc tataacagaa ggtttaaaag ggatacttga agatgtagaa
     1621 aatggaaagt tagtattgga tggagagtat gaagatatac atagttttac tgagataaat
     1681 ttaattcaaa gaattggaga tgttggcaag aagcttcata cagcacgtag tagaaatgac
     1741 caagttgcag ttgatatgag attgtatgct aaagaaaaag caaatgattt ggtggattta
     1801 atatctgaat ttaaagctac tataaaagat gttgctgaca aaaacccagt gatgatgcca
     1861 ggctatactc atcttcaaag ggcacaagta gtgacattta agcatcactt aatggcatat
     1921 tatagtatgt ttgatagaga tgagaaaaga ataaaaaatg ctatagaaat attagatgaa
     1981 tcacctttag gctgtggagc tttagcagga actacgcatg atatagatag aagtataact
     2041 tgtgaacaat taggatttaa aaaagtagta gataatttta tggatggagt aagtgataga
     2101 gattatttac tagagctaat gagtgatttt tcaattataa tgatgcactt aagtagatta
     2161 agtgaagagt tgatactttg gagttctcaa gaatttggat ttgttgagat agatgattta
     2221 tatacaactg gaagtagtat aatgcctcag aagaaaaatc cagatggtgc agagcttata
     2281 agaggtaaga ctggtagagt atatggaaac ttatttggtc tttttacagt aatgaaagga
     2341 ataccattag catataataa agacatgcaa gaagataaag aaggtttttt cgatagtgta
     2401 catactcttg aaatgtgtat acaaataatg gatagaatga tagccacact aaaggtaaat
     2461 gaggataaaa tgaaacaagc agtaaaaaat ggttttttaa atgcaacaga agttgcggat
     2521 tatttggtta aaaataatgt tgcttttaga gatgcacatg gtatagtagg aagtatcgtt
     2581 atatattgtg aagataataa aaaagcaata gaagatttaa cattagaaga attacaaaag
     2641 ttttcagatg cttttaagga agatatttat gattttatcg actatgagag tatattaaat
     2701 aagggaataa aaaagaattt aaaataatgt atttattttt aaaagctgta aatttgaatg
     2761 ttatcattta aatttacagc tttttattgt gtaagatttt attatataaa agtaagaaac
     2821 ttaaaaggag tagtatttaa aataaataca aataaaaaat tattattaaa atgataatta
     2881 atatcaataa gtattgacaa aattatttct aaatactatt atatataata taaataataa
     2941 tgaaaatcaa tatcaaataa atgggaggaa atctaatgaa cttattagtg attggtggtc
     3001 atgaaagaat ggaaaaagat tatatggtta tggccaagaa aaaaggttat aagacaaagg
     3061 tatatactac catgtcatct aaattaaata attctatagg aaaaccagat gcagttgtca
     3121 tacttacatc aactgtttca cataagatgt ctagaacggt tgagtctcaa gctaaaaaac
     3181 aagatatatt aatcgtaagg cataaaaata gtagtaaggt agcatttaat gaatgtctag
     3241 atatggttga tgaatgtctg ggaaattgtt caaaatgtaa gcttaaagac aaacaaaaac
     3301 aaaaaaatat ataggtataa aaaatacata aacataaaat taaaagtact gagataatag
     3361 ttataattat tatctcagta cttttagatt tataaggata agtatagtat aaatttttta
     3421 ttataatttc caactacttg ttactttttc atattttttt tacttctagc tttttttctt
     3481 tttttcttct ttatatttga agctattata aataaaacta aaagcactag aatacctgca
     3541 ataatcttaa caaaagtcat taaaacatta ttttcagtca aaaatggaag actgctatca
     3601 agattatttt ttgcaattaa gtctatactg ttttctagtt taccattatt atatacattt
     3661 aaagtaccaa ccttagctcc tttttttata ggcaaatcaa ttttatttaa ctctgttttg
     3721 gtatcataat tgccttgaga ttcatctttt tctaagaata tcctataact attttttggt
     3781 tcataaatca attcatgttg ttttgtaaac aatacttttt tagtatctgt gtatttttct
     3841 ttatcaacaa tagtagcaca cttaaagtta tcaaatccat aatcaatcag aatacgtgag
     3901 tctaaatata aatcagcatt agttgaatta aatacagcaa cgataactct tctgccatct
     3961 tttacagcac tcgataaaag acatttacca gcagcatcag tatatccagt ttttatacca
     4021 tcaactatat catattttat atttataggt tgacctttgt atgtaatctt atcatgtgat
     4081 gtcaaaaact tattagtgtt tacaaagtat ctctcataag gatatgcttt tgtagcttca
     4141 aatttaagtg acttagtttt tacaatttgt ctaaaagtat cattattcat agcttctcta
     4201 gctatgagag ccatatcatg agctgttgtg tagtgatttg ggtctggtaa tccatgagga
     4261 ttattaaaat gagtattagt agctccaatt ttttttgcct cagagttcat gaggtcaata
     4321 aatttttgta cattgccacc accaacatat ctagctaata caaaagcagc atcattagca
     4381 gaatgaacca aaagagcatc tagtaactgc tttacagtaa aagattcccc attttttaga
     4441 tacatactag agccatctat ttgaggtaaa tctttaattt ctattacttg atttaaatct
     4501 ttaacctcct ttagaactaa acaagctgtc catactttag ttgttgaagc tggataaagc
     4561 ttagaatttc catccttatt atatagtatt ttgccagact catagtccat taaaactgca
     4621 tgtttagcaa ctatagctgg ttcattatct gcaaaagaga tattagagct tcctaaaaat
     4681 gaagtaaata tcaataaaca gattaataaa agtgataaat ttcttttcat tgtgagcctc
     4741 caaattttaa attcatcttt tagtatagca tatatttgtc aataattaaa agaaaggagt
     4801 gattaatata tgaaaaaaaa ggtaaaattt atttcgtttt ttgttttatt tattttaata
     4861 atatcatcaa taatcgttta taaatcgctt attttcaaag atgatattta tgttgtaagt
     4921 aaaaaaaatt tacctaatga aaaaacacat gttgtagaga atgaaattaa aggtgaagat
     4981 aataatcaca gtaaagtaac taaagacata gataaaaatt ttcaaaataa taaaataact
     5041 atatacataa gtggagcagt aaatagacct ggaattgtaa ctatagagtc agacaagaga
     5101 ctttatgatg ctgtagaatt actaggagga acgacaaaag aagcagattt aaatggagtc
     5161 aatctatcag taagattaga agatgaacaa cattacataa tacctaaaat aggtgaagca
     5221 acatctgtta caagtaatga tgattccaat aaactaaatc agaagaatga gtcaagaggt
     5281 gagcctaaaa ataaatccaa taaaaatata tctaacgaat caaaagtaaa tataaatgta
     5341 gcgacgatag aagagttgga ttctctacct ggggttggag aagctacagc caataaaatt
     5401 ttacaacaca gagaagaaaa tggacagttt agttcaattg aggaaataaa aaatgtaaat
     5461 ggtattggag ataaaaagta tgaaaatata aaagatttaa tttgtgtaga ctaaaatata
     5521 atacaaaact tacatataga tatgttatta taaatatgta tgtatatgtg tgtacatgtg
     5581 gaaatttagt aaaggaggca cttttatgaa aaaagaacaa aaaaaactta cagagatgac
     5641 aacagcaggt ggttgagccg ctaaaatagg gccagaggtt ctggcatcag ttttatctca
     5701 attgcctaaa aatgataata tagaaaattt attagtagga ctagatacag cagatgatgc
     5761 tgcagtttat aaattaaatg atgatatggc acttatccaa actttagact tctttacacc
     5821 aatggtagat gacccatacg tatttggtca aattgctgca tcaaattcac tatcagatgt
     5881 atatgctatg ggaggcaaac ctttggttgc tatgaatatt gtttgttttc catcatgcca
     5941 tgatatggat gtacttgcag aaatattaaa aggtgggttt gataaagtga aagaaagtgg
     6001 agcacttttg gttggagggc atactgtaga tgacaaagag cctaaatatg gtctttcagt
     6061 ttctgggatt gtaagtccaa ataaagtttt atcaaatgct actgcaaaac ctggtgataa
     6121 gttaattata acaaaaccaa ttggagtggg tgtattaaat acagccatga aagaaggtat
     6181 ggtagaacaa catatagcag ataaagtaat agaaataatg atacatttaa ataaatatgc
     6241 agcaatgagt tttgagaaat ttgatgtaaa ttcggttaca gatataactg gttttggtct
     6301 acttggtcat actttagaaa tggccaaagc ttctgaagta agtattgaaa ttgaatcaaa
     6361 acatgtacca attattgaag gtgcaataga aatggcacaa atgggaatta tacctgctgg
     6421 tatgtataaa aatatgcatt atgtatctaa agatgtagaa gttgtaggaa atatagaagt
     6481 tgcagttcaa gatattttat acgacccaca aacatcagga gggttgttaa tttcagtaaa
     6541 agaagaactt gcagaggagc ttgtaaaaga tatgaaatta aatggagcaa tagaagctaa
     6601 aattataggt tctgtagttc caaaagggga aaaatatatt acagttttat aaaatattat
     6661 agagaaagag tgattgtttt gagcaaaaga gaattgtttg caatgttgcc ttcagttgat
     6721 gaagtattaa gtgataatag aattgtagaa ataataaatg aatatcctag aagtttagtt
     6781 ttagaatctg tgagagaagt aatagattta aaaagacaat ttatattaag attaaaagaa
     6841 gatgcatcaa actctgtaac aatagagttt ggtgaaataa tagaatctgc aatagagaga
     6901 gttaagttaa attactcatt atctttaaaa aaagttataa atgcaactgg tacagttatt
     6961 catacaaatt taggaaggtc tttacttagt gaagatataa aagatgaatt atggtgtgct
     7021 gcttcaaggt attcaaactt agaatacgat ttggataatg gagaaagagg ttcaagatac
     7081 tctcatttaa ctagtacaat aaaaagactt actggagcag aagatgtact agttgtaaat
     7141 aataatgcag cggcagtact tcttgtactt agcacaatgg ctaaaggagg agaagcaatt
     7201 gtatctcgtg gagaattagt tgaagtaggt ggttcattta ggattccaag tataatggct
     7261 cttagtggtg cagaattagt tgaagttggt tcaactaata aaactcattt gaaagattac
     7321 aaggaggcca ttacagaaga tacaaatgta ttgatgaagg ttcatacaag taattataga
     7381 ataatgggat tcacagagag tgtttctata gaagaattgg ttaatttagg taaaaaatat
     7441 aaactacctg taatagaaga cttaggaagt ggagttttta tagatttatc aaaatatggt
     7501 ttatcttatg aaccaactgt attggattct attcgacaag gtgcagatgt agtaactttt
     7561 agtggagata agatgcttgg aggtccgcaa gcaggtatta tagttgggaa aaaagagtat
     7621 atagaaaaaa tgaagaaaaa tcaacttact agagcactta gagttgataa gcttacaata
     7681 tgtgctcttg aagcaacatt gagaatgtat ttagatgaga caaaagctat agaaaatata
     7741 cctactttaa aaatgttgac atataaaatt gaagaattag aagtaaaagc aaataaacta
     7801 tttgaaaaaa taactgcttt aaacttaaat gcaaacataa atatagaaga tggattttca
     7861 caggtgggag gagggtctat gcctcttgaa actataagta caaaggtaat atcaataact
     7921 cctgaacata tgaatgtttc atctttagaa aaaaaactaa gacttagtga agcacatata
     7981 atagccagag tgtatgataa taaatatgta ctagatgtaa gaactatatt tgatgatgaa
     8041 tttgatgtaa tagttgagga attgagaaaa gcatttaatt aaataaattt gagttaataa
     8101 atattaaatt tagaagtata agtatgaagt tttgattaaa aaatagctac acacacttgt
     8161 gactttagtc atagcaggtt tagagggggc aaaaaatgaa aaatgtagta attggaacag
     8221 caggtcatat tgaccacgga aaaactactc ttataaaagc attgactgga agagagactg
     8281 atacgttaga tgaagaaaaa aagagaggta tttcaataaa cttaggattt acttattttg
     8341 atttaccaaa caatagaaga gctggaattg ttgatgtccc aggacacgaa aaatttataa
     8401 aaaacatgtt agctggagca tcaggaattg atatagtttt acttgttgta gcagcagatg
     8461 aaggggttat gccacagaca gttgagcatt tagatattct aagcttttta aatatcaaaa
     8521 atggaatagt ggttctaact aagtctgata ctgtagatga tgaatttaga gagctagtaa
     8581 aagaagatat ttgtgaaaaa ataaaaggaa cttttcttga agatgctgaa ataattgaag
     8641 ttgattctat atctaaaaga ggtatagaca ctcttattga aaaaatagat aaaatgagtg
     8701 atgatataga agataaaaat gaaaactctc cagcaagact taatatagac agagtatttt
     8761 caataaaagg gtttggtact gtaataacag gcactttaat agaaggaaag ataagtatag
     8821 aagatgattt ggttatatat ccacaggtat taaaaacaaa aataagaagt atacaagttc
     8881 atggagaaaa taaagataca gcttatgcag gtcaaagaac agcaatcaat atatcaaatg
     8941 taaaagtgga tgatataaaa agaggagatg ttttagctgc accaaactca ttggaggaat
     9001 caatgatgtt agatgtaaaa ttatcattgg taaatcattt agataaagga ttaaagcact
     9061 gggatagatt aagattgtat catggaacta gagagatact ttgtagagca gttccactag
     9121 ataaagaatt aattgaaagt ggagaaagtg gctatgttca gttgagactt gaagaaagca
     9181 tagtatctaa gaaaggtgat acgtttgtag tgagacgtta ttctccaatg gaaactatag
     9241 gtggaggtgt aataattgac ccatctccta aaaaacataa gaaatttgat gaaaaagtta
     9301 tagaagcatt aaaaataaaa gaaaaaggcg aactaaaaga tataatagaa gagtacttaa
     9361 aaaggaattt aaaaaattat ccaaatataa aagaaataat gagctatagt ggtgctcatg
     9421 aagaagatgt aaaaagagcg cttgaaacac ttatatcaga agataaagta tttattatag
     9481 gaaatatgta tatgcatata aatcaataca ataaattaaa agaaaataca ataaagcttt
     9541 taagtgagta ccataagaag tataggctta gaaaagggat actaaaagaa gaagtaaggt
     9601 ctaagataga gagtaatttt aagactaggg agatggatat tttattagaa aaattgtcta
     9661 cagaaaatgc tataaaaata gaaaataata tagtatctct attagatttt gaggttattt
     9721 taaatgataa gcaaaaagaa atagcaaaaa aaatagaaaa aagattaaaa tcatgtggag
     9781 tttcttctat actgacaata gatgaagttt ctgaaggaaa tcataactat gctgaagtat
     9841 tggagtcaat gataggtaat aaagtagaga aattagatga tttgtatata atggacaaag
     9901 atatttatga aaatgcaaag aacattttga taaattatat aaaagaaaat aaagaaataa
     9961 cattaggaga atacagagat ttgattgatt ctagcagaaa gaattgcatg attatattgg
    10021 aaaacttcga tagaaacaaa ataactaaaa gagtagaaaa caaaagaatt ttgttttagc
    10081 agaaaatgtc tgtttttgct ttaaggtata aagaaggaat tataatgtcg agtatcgtat
    10141 aattaattag tataaaatat taattaagag gaagtgtaaa gtattgaaga atataatacc
    10201 tgatgtaaaa ccagatataa aagaaagaga agacatgagc cttttattta taataatggg
    10261 ctcagtactt ttattattta ttataaaaga tatagagaaa tatgaaactt tattacatgc
    10321 catatatacg atatttttag tagctacagg agtaattata tttaatacta caaaatttaa
    10381 agtagattca ttttctacat ttttaggatt aatttttata gctactggag tattggagtg
    10441 tatatattta tttaattcct tggggattaa aactaagtct ataatggaga ttaacatcac
    10501 aatatcagca ataacagatt tatttcctat actgggtgta tacttaagtt ttaaatttgt
    10561 taaggataac aaacaaattt attcaagtgt aattttattt atagcaactg ctacattaac
    10621 aataacatct ttattcataa tatcaggaat ttgtgactat ctgggaaatg gtaaatcaca
    10681 atatgcttta ggagttataa tatctatatt cataataatt atttctatca ttagtggaat
    10741 tgaattaaat tcaagttcaa ataaaggtaa atgggaatat gatgaaaaaa agatttttaa
    10801 cagaataata gtaataataa tcttatcaag agttccaaat ctattgcata tagttattga
    10861 taatagaaat gtagagagga tattatcaca aataataaca aatgtagcct tatattattt
    10921 atataattac atagtatcta agaatattaa gaaaacagca ctggaattgc atgatacgaa
    10981 tgaagaatta acaaaaaaaa cggaaagttt aaaagaaaag aataaaaaat taatctatga
    11041 gacagaaaag atagaggaac ttaagggaat acttatgaaa agagaatcta ggctacaatc
    11101 tacattagat gtagcagtaa atagcatagt agtatttagc aaagatggag aaattacata
    11161 tgcaaataag agctttagaa atacttttgg agactataaa gaacaagata aattgactga
    11221 taaaataaaa aacttcaatg atttgataga aagcattcat tatgtgtttg ttcatgagaa
    11281 gaatgcagag aaacttatta gtacaagcga taataaagtt tatcaagcta tatttgcacc
    11341 tttaattatt gcttcacaaa cagaaggagt attgtgtgta ctagaagata agactaagaa
    11401 aaaggagtac gaacgcaaac ttattgaagc aaataagaga agtaaagatt ttttagaaag
    11461 tattggagat ggcatagtag tgcttgaagg taataacaaa atatatgtta ataatgcatg
    11521 tagggaaatt tttaaaaata atttagagag tattgatttt tcattagtat gtaaaagtga
    11581 agaaagtata gaaaaaagat atgtaataga tggtgaagta aagtatgtag agatgagttt
    11641 ttctcagtat actaatgctg gtactaataa aactattatt gtaatacgtg atacaacctc
    11701 tcgaaaaata tctcaaataa aacttgagga aagtcaatca tcatattcaa gatttataga
    11761 tatattacct gatggcatat gcttattaaa agaagattta agtataaact atgcaaataa
    11821 gtcgttttta gatatgctag cttttacaaa catagatgat ataaaagatt ctaatattaa
    11881 actattaatg aatgcgaata ctgaagaaaa aatgaaattt acggataata tggctaaagt
    11941 tttaaatgaa aatagaagta tacttttact ggaacatgaa ttgataaata gttatgatga
    12001 tatagtagaa gtagaattaa gtgcattacc ttttgctata tacaatacta gatacataat
    12061 gcttattata aaagatttag tacataagaa atattccgag caagcagaaa aagaactgct
    12121 tgaaagattt aaaacagata aaatcaaaac ggagtttttt gcaaatatgt ctcatgaact
    12181 aaaaactcca ctaaatgtta tatctagttc aaatcaactg gtagattctt tctatagaaa
    12241 tgaaaagata aaagattaca ataacaatat taaatctcat gtggatttag ttagacaaag
    12301 ttcttatagg cttcaaagat taataaataa cattatagat ttaacaaaga tggagtctgg
    12361 gttctataca ctaaaacttg cgaaatacaa tattgtatct gtaatagaag acttatttat
    12421 gaatatagaa gagtatgcac ttagaaaaga cataaaaatt ctttttgata cagaccttga
    12481 agaaattaat gtttatatag acaaggttga aattgagaga attatgttga atctattgtc
    12541 aaactgtatt aagtttactg ataatggagg ctggatatat gtaagtattc attataaaat
    12601 agataaagta atcataagtg taaaagacac tggagttgga ataccacaag ataaattaga
    12661 gttgattttt gaagaatttt cacaagttga taagacttta tccagaaata ctgaaggaag
    12721 tggaataggg cttgctatag ttaagaatct agtaagtctt catggtggag atatagaagt
    12781 agtaagtgaa gtgaataaag gcacagagtt cttaatttca cttccaatga agggatttag
    12841 tagtgaacat tacacagaag ataagagaat atataatatt caagaaaaaa taaaaataga
    12901 attttctgat atatattatt aaaaatgatt atgagcaaaa ataaaataaa aatactattt
    12961 gtttaaatat ttaagttgta agaaataggc tatcataagg taaattatta tgatagccta
    13021 tttttagcta ggttttatat ctatatgtga gaacatataa tctctaaatt accttcaaaa
    13081 ctaaaatctc caaagtcgtt tggcagtata aaatgcatac ccttttttaa attatattct
    13141 acagaattgt gtataagttt tccatttcca tccaacacac tgtataaaga aaaaggtgta
    13201 ttgtgtgtaa aattacattt tccaaaaaca tctaatttat aaacactaaa aaattcgttt
    13261 gacacaaatg ttgtgcattt aaagtctgat gttgatacga ttttatagtc agtatcgaaa
    13321 tttatatgag gaacattcgt aacatctatt gatttttgaa catgaagttc acgcttatta
    13381 cctgaattgt cagttctatc ataatcatag actctataag tagtatctga gttttgttga
    13441 gtttctaaaa tcaatgttcc tttgcatata gcatgtatag tgccacttgg aacatagaaa
    13501 aagtctccct tttttatatt aacctttcta agtagtttgt cccattcatt attatttacc
    13561 atatttacaa attctttatg agattttgca ttatgaccta ttataatttc tgcatcatca
    13621 gaacaatcta ttacatacca acactctgtc ttacctaact ctccattttc atttttttta
    13681 gcatattcat catttggatg aacttgaact gataaattat catttgcatc caatattttt
    13741 gtaagtaaag gaaacttatc accaggtgtg tttccaaata attctctatt tttattccac
    13801 aattcagata gttttttacc tttatatttt ccattttcta ttaagcaatc tccattttta
    13861 tgagagctta tagcccaaca ttcaccagtt gttggcgaat ctatttcata attaaattta
    13921 tctttaagtg cagtaccacc ccatattcta tccataaaaa taggttttaa aaataatggt
    13981 tccataatcc tgttcctcct atgatattaa atttatccta caaataatta tatataaata
    14041 gattataaaa tcaatagtaa aaaagttttg tcccttgaaa tgacataaag tataaggagt
    14101 tattaaataa gataaactat gatatataat ataaagttct aaattggagt gataattatg
    14161 gagttgttta aggcagaaag caaaaaatat aaaaaagaat ttttaaaatt attggagcaa
    14221 tgggttagta taccttcatt ttatgataga aaaactgtat ctaaagacat gccttttgga
    14281 aaaggagtat atgatgcatt aaactggttt gaaaatctag gtagagaaaa caatttcaaa
    14341 gtaaaaaata tagataatca tgctgttcaa attgaatatg gaaatggtaa agagtatgta
    14401 gatatatttg gacattgtga tgttgtaaat ccaggtgaag gatgggatag tgaaccattt
    14461 aaactaaata ttataggtga taaattagtt gctagaggtg tatctgataa taaagggcct
    14521 atgatagtga attttttagc actaaaaatg ataaaggatt tagatattaa tttaaaaaga
    14581 aaggttagat taatagctgg gggaaatgaa gagagtggat ttaaatgtat aaagcattat
    14641 tatagcaaag agccatatgg tgtatgtgga tttactccag atgctaaatt tcctgtatta
    14701 aatggagaaa aaggtggagc aataataaag ttaatttcaa atatagacga taaatcttta
    14761 tatataagtg gtggaataga gtttaataca ataccagata aagtttatat aaagaatgta
    14821 gaaaaattgg gtaaagataa tatatgtttt gatattaaca atatatcgat aaattatgat
    14881 aatggcaatt atattgtgta tggaaaaggt ggtcattcat ctaaacccga aaaatcaata
    14941 aatcctatat tagctacaat taaacttttg tcagaaaata ttgatgaaaa atggacaaaa
    15001 gatttatata agttgataaa tcaagataat ataaatggaa acttatttgg tttaaatatt
    15061 gaggggaaat gtggaatttt atcaatggtg ccaacaataa taaatatagt agatggaaaa
    15121 ttagaagtag ttttaagcgt tagatatcca gaaatattaa ccattgaaga tataataaaa
    15181 aaatttaatt tgtatatgga gcaaaataat attaataaat ttgagcttat tggggaaaat
    15241 ttaaagcaag caaattatat agatagaaat tcaaaacttg taagaagttt gcatgatata
    15301 tatataaagt attctggaga tttaaaaaat gatgttagag taactagtgc aggaagttat
    15361 gcttctgaaa tgaataattc agtaatattt ggttgtgaat ttcctgatgg tagctttggc
    15421 aatgtccata gtgcgaatga atttgctagc ttagatagat ttataactgc aataggtata
    15481 tatgcagagg caataattac attatgtaat aaaatataaa tacaaaatgt cccttatata
    15541 gacatattat atatacaatt aaaattttac ttgatatata attgaaatca aagagaaggt
    15601 gatacaatga taactaagaa acatgcaata atagtaaaga gtttaagcaa taaagatgga
    15661 tatatgacat caaacgaatt agctatcaaa ttggatgtgt ctactaaaac tattaaaaga
    15721 tatatagcag atttaaatag tgttttaagc aaatatgatt tggtaatagc ttcttctaga
    15781 ggtataggat acaagttaag cggttctaaa aacaatatag ccagagctgt taaagaagca
    15841 aataaatata ttgatggttt tttagatgac tctgaagagt caagaatgtc aaatataata
    15901 tgtatgctta ttaatagaaa ttatatgagt atagaagcaa tggcagaaga attaaatcta
    15961 agtatagccg ctataaataa attgtctagt aagcttaaaa gaaaacttga aaagtatgac
    16021 ttagttataa aatctaaacc ttactatgga agctatatac atggtgaaga aattaatata
    16081 aggcaattaa ttactgacta tgcaataaaa ttagatgaaa aaagtaaagt gaaggtgttt
    16141 ttagatgaca ttagtgaaaa cgatatacac tgtatagaaa gtattttaga aaagcactta
    16201 agagaaaaag atacaataat ttcagataaa gattttaatc ttttgctttc aaaaataatc
    16261 gtttctgtat ttaggagtaa gagaggtcat agtaacaata taaacttaat ggatacaagt
    16321 tatagatttc ataactacta ctttatagaa aatctaatga aagaaatatc tgataaaatt
    16381 ggatttaaac ttattgaaga tgaagtaatt tatatttcaa attattctgg tgtaatagcc
    16441 tataaaggta cacaaggaag aaaaaacaca agtgaaattg aggaacgaat aagtagtgtt
    16501 ataagtagtg cacttcaaga tatattcctt atttcaggta gtgattatac aaaagatgat
    16561 gagtttatgg ttgcaatctc tgaccatata aagagatttt taaatagagc tagagctaat
    16621 gtaaaatcaa ataatccttt acttcatcaa ataaaagaga agtttcctat agcttttaat
    16681 ttagccgtgt ttttatcaaa taaactagaa actgagttta atttaaaact tgatgaagat
    16741 gagcttggat atatagctat ccactttgct gcttcaaatg aaagaatgaa aaaaaatact
    16801 agtaagaaaa tttgtatagt ttgtcattat gggataggaa caggacaact tatttctgaa
    16861 aagctaaaac agaatataag tgatttaagt gttgtaggtg tttatccagt tagatatctc
    16921 gatatggcta taagtcaaga tgttgatttg atagtatcta ccgttgaatt aaaaggatat
    16981 gaaaaacctg tcttatatat agaaaatata tttgatgata gcttaattga aaacgtaaac
    17041 aaagcctttt atgaaaagga agaaagaaga aagataataa gcaacatgtt tgatgaaaaa
    17101 gcttttttca gtataaaggc taatactaaa gaagaggtca taatttcact ttcaaataaa
    17161 ctaaaagaaa gagattttat agaggaaagt tctataaaaa gtataataga tagagaaaat
    17221 atttcatcaa ctgaaatagg aaatctagta gcaatacccc acacaatagt gaagggagat
    17281 aaaaaatcta taataggagt tggaatactt gaaaatccaa taatttggga taagcaagag
    17341 gttcagctag tatttatggt attttttaat actaaagaaa aacataattt ctcgatattt
    17401 aaataccttt ataattttat aaaagatgaa ggcggagtaa gaggaattat taaaatatgt
    17461 gactttaata aattaatggc actgataggt aattaaggag gaaattaata atgggaacaa
    17521 acatatttaa caaagaatat gtatttttaa atgttgatgc taagtcaaag gttgaagttt
    17581 taaaatttat atcaaaaaaa gctaaagact taaacttggc agaagatgag agcttagtat
    17641 atgaaggttt aatggctaga gaagaacaat ttactacaaa tttaggagaa tctatagcaa
    17701 tacctcacac taaaaatgat gcaattgaaa atccagcagt agtagtattg aagtttaatg
    17761 aagatgttgt ttggaatgaa ggtgaggata aggttaaatt agcaataagc ttacttatgc
    17821 caggaaaaag taaagagaat attcatctta aattattatc atctctttca agaaaattaa
    17881 taaataaaga atttaaagat tctcttttaa aaagtgataa tgttgaagaa atatctaact
    17941 taataaatga agctttaggt ttataattaa acactaaggt tataaaataa aaaaataatt
    18001 aactaaaaat aggaggaatt tgaaatgaat aaaaaattag tagcattatg tgcttgtcca
    18061 atgggactag ctcacacttt catggcagca gaagcaatag agcaagcagc aaaggcatta
    18121 ggttatgaag caaaagttga aactcaaggt gctgatggtg tacaaaatga gttaacaaga
    18181 gatgatatat taggagcaac tatgataata catgcagtag caataacacc agaaggtatg
    18241 gaaagatttg atggttgtga agtttatgaa gttgaattac aagaagctat aaaaaatgca
    18301 gaaggtgtta taaaagaaat agaagaagat ttaggaatat aataaattta ttgtggagga
    18361 aatagaatgg caattaagaa aaaagttata aacagttcag gtgcttctac aacaggagga
    18421 aataatgtag gaaaatctac tccatctagt ccaactcaat caaagggaaa tggaaaatgg
    18481 aaagaaataa gtaaacatat aatgacaggt atatcatata tgataccagt tttagtaatg
    18541 ggtggactta taggggcctt atctcaatta ataccatatg ctatattagg acttgaccca
    18601 tctgtaggta tagttgatgc tatgaactca ggagaattta cagggttcaa gcttagtctt
    18661 ttaaatatag cacaattaat gtcaaatttc ggatttactt tgtttggatt tgcgatacca
    18721 ctgtttgcag cattttgtgc aaactctata ggtggaaaaa cagctttaat tgcaggattt
    18781 ataggtggat atatagctaa taaacctgta ggtgtaccac aattcgttga tggacaatgg
    18841 actgaagttg ttccagttgc atcaggattt ttaggtgcaa tactaatagc atttataata
    18901 ggatattttg taaaatggtt aaataaatct ataaaagttt ctcataactg gttagctttt
    18961 aaaacaacat ttttaatacc attaatagcg tcattagctt gtatggtatt gatgatattt
    19021 ataataactc catttggtgg attaattaat gaaagtatga aaaacttctt aacagcagca
    19081 ggagcagctg gagaatatgt atatgcaaca gctttggcag cagctacagc attcgactta
    19141 ggaggaccaa taaataaagc agcaggattc gttgcacttg gtttaactac agagaatgta
    19201 cttccaataa cagcaagaac aatagctata gttgttcctc caattggatt agggttaaca
    19261 acattattag ataaaagatt agtaggaaga agagtatatg atagacaatt ctatcaagca
    19321 ggaaaaactt ctatattctt atcatttatg ggtatatctg aaggagcaat accatttgca
    19381 cttgagagac caggttttgt tataccttta aacatagttg gaagtgtaat aggagctatt
    19441 acaggtataa ttctaggagc aattcaatgg ttcccagagt cagctatatg ggcatggcct
    19501 ctagtagata acttatttgg atatataata ggtatagcag ttggtgctat attcatagca
    19561 gtaggaaata tattctatag aaataaatta ataaaagatg gtaaacttgt tgtagattat
    19621 atcgactagt atttaatttt tactatctat aaataacttt ggctggtaat atggttttaa
    19681 tcaatattac cagccttacc tatatgatta attaaaggcg gtggagataa gatatggata
    19741 agatagaaaa agtaagagag tattttagag aatatgatat agatggattt cttataaata
    19801 gtcctacaaa caagttttat gttggaaatt tatttagctc ttcaggttat gtttttataa
    19861 cgaaagaatc acaatatata atagtagatt ttagatattt tgaagaaatt aaaagaaaat
    19921 ctagtttgtt taatgtagta ttaatggaca aaactagaac tcattttgat ataattaatg
    19981 atatttgtag agagcagaat ataaaggaaa ttggatttga gggaaatgaa gtatcttttg
    20041 atttatatag aagtatgagt aataagttaa gtgcaacctt aaaatcagtt gatttatcta
    20101 cattaaggga aacaaaaaat gaagacgaaa taaaatatat aaaaaaagct tgtgaaatag
    20161 ttgatgctac attttatcat atagtagact ttataaaggt tggtatgact gaaaagcaag
    20221 ttgaaaatga aattgttaga attataaaag atttaggtgg tcaaaaagaa tctttcgata
    20281 caatagttgc atctgggctt agaggagcat tacctcatgg aaaagcaagt gaaaaagtta
    20341 tagagtatgg agattttgtt acctttgact ttggtgctaa gtataataat tattgttcag
    20401 atattaccag aaccatttgt atgggtacta taaataaaga gttagaagaa atatataata
    20461 tagtaagaaa agctaatgaa gagtgtataa gggttttaag accgggaatg actactggag
    20521 agatagataa agttgcaaga gacataattg gttcatatgg atatgctggt aattttggtc
    20581 ataatctggg tcatggtgta ggaattatgg ttcatgaata tcctgcatta gctcctgaat
    20641 caaatgaagt attaaaagaa ggaatgattg taactataga gcctggtata tatgttccaa
    20701 gtcttggagg cgtaagaata gaagatgatg tacttataac acaagatgga tgtatgagat
    20761 tgactacatc tactaaggat ttaatagttg taagctaaaa ataaattaga taaatatttt
    20821 ataacaataa gaaaagaaga ggtaaattta aatgtataat tttgatgata aaccagatag
    20881 agtaagtgaa aagtgtagaa aatgggattt aaatattata agagataaat ttggagatat
    20941 tagagaagat tttataccaa tgtggattgc tgatatggat tttaaaatac caacagaaat
    21001 agaaaacaaa tttattgaag ctgtaagaag aggtgtattt ggatatacat actgttatga
    21061 tgaattttat gattcagtta ttggttggca aaaaaatatg catgaggtaa atgtagaaaa
    21121 agaatggatt acattaactt atggaactgt atcaacactt cattatgtag tacaagcttt
    21181 ttgtaagaaa ggtgatagta taatattaaa tacaccagtt tatgacccct ttgaatcatc
    21241 agcaaaaaaa caaggtgtaa atgtaatttg taatacatta gatgttgtaa atgatagata
    21301 ttatataaat tttgataaat tagaagttca aattaaagaa aataagccta aattaatgat
    21361 gttttgtaca ccacataatc catctggaag gatatggaca attgaagaaa tgacaagggt
    21421 agcaactata tgtaaagaaa ataatgttat tttggttgca gatgaagtac atgcagagca
    21481 tattcattat ggtacattta attcaatact taagattgga aaagagcttt tagaaaatgt
    21541 tattctattg acatcaccaa ataaaggatt taatcttggt ggattaaaga cttcttattc
    21601 aatagttata aataaggata taagggacaa atttagaaat aaattaaagc aaaattctat
    21661 aacttctcca aatgtgtttg gaataatagg attgataacc gcttataatg aatgtcatga
    21721 gtggttaagt ggtgtaaatg aatatataaa atcaaattat gaacttttag aaacttgggt
    21781 aaataaatat aataagataa aattgatgaa aatggaatca tcttacttag cttggatgga
    21841 tataagtgga ttaggtatta gtgcaagtga gtttacagat aaattggcta tagatacagg
    21901 agtattatta gaagatggga gtcattttgt aaaagatggt gagaagtatg ttagaataaa
    21961 tttaggaact caaaaggaaa atgtaattga agctttaaat agaatggatt tatttttaaa
    22021 gtctttataa aaatttggtt gaattagtat tttatataaa tttaaactat taatatggta
    22081 tatacataag tagaatttgt tttagattac ttaagtatat accatatttt atataaaaat
    22141 taaatttgca ttagtcatgt gataactatt taaattcata tgtattttgt atgtataata
    22201 gatataatta ttatttaaac t
//
LOCUS       DAYAOX010000046        21922 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.046, whole genome shotgun sequence.
ACCESSION   DAYAOX010000046 DAYAOX010000000
VERSION     DAYAOX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.046"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(160..837)
                     /locus_tag="ACGI57_001384"
     CDS             complement(160..837)
                     /locus_tag="ACGI57_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing C40 family peptidase"
                     /protein_id="HFG9716465.1"
                     /translation="MKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMSATVDQCDFLN
                     VRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDYLTIQGGNVDA
                     KVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRVSRSQATVGKK
                     VSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMAPGTNYARRLI
                     TATRVLQ"
     gene            complement(1066..2979)
                     /locus_tag="ACGI57_001385"
     CDS             complement(1066..2979)
                     /locus_tag="ACGI57_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFG9716466.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(3063..4154)
                     /locus_tag="ACGI57_001386"
     CDS             complement(3063..4154)
                     /locus_tag="ACGI57_001386"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9716467.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(4160..5527)
                     /locus_tag="ACGI57_001387"
     CDS             complement(4160..5527)
                     /locus_tag="ACGI57_001387"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HFG9716468.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            complement(5481..6233)
                     /locus_tag="ACGI57_001388"
     CDS             complement(5481..6233)
                     /locus_tag="ACGI57_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9716469.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(6281..7189)
                     /locus_tag="ACGI57_001389"
     CDS             complement(6281..7189)
                     /locus_tag="ACGI57_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9716470.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(7211..7960)
                     /locus_tag="ACGI57_001390"
     CDS             complement(7211..7960)
                     /locus_tag="ACGI57_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9716471.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(7989..9164)
                     /locus_tag="ACGI57_001391"
     CDS             complement(7989..9164)
                     /locus_tag="ACGI57_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFG9716472.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(9179..10018)
                     /locus_tag="ACGI57_001392"
     CDS             complement(9179..10018)
                     /locus_tag="ACGI57_001392"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9716473.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(10021..11178)
                     /locus_tag="ACGI57_001393"
     CDS             complement(10021..11178)
                     /locus_tag="ACGI57_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HFG9716474.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLSIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(11194..11916)
                     /locus_tag="ACGI57_001394"
     CDS             complement(11194..11916)
                     /locus_tag="ACGI57_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9716475.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(11928..13001)
                     /locus_tag="ACGI57_001395"
     CDS             complement(11928..13001)
                     /locus_tag="ACGI57_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HFG9716476.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(13023..14729)
                     /locus_tag="ACGI57_001396"
     CDS             complement(13023..14729)
                     /locus_tag="ACGI57_001396"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFG9716477.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            complement(14775..16331)
                     /gene="murJ"
                     /locus_tag="ACGI57_001397"
     CDS             complement(14775..16331)
                     /gene="murJ"
                     /locus_tag="ACGI57_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFG9716478.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            complement(16446..17501)
                     /locus_tag="ACGI57_001398"
     CDS             complement(16446..17501)
                     /locus_tag="ACGI57_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HFG9716479.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(17596..18282)
                     /locus_tag="ACGI57_001399"
     CDS             complement(17596..18282)
                     /locus_tag="ACGI57_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFG9716480.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(18502..20526)
                     /locus_tag="ACGI57_001400"
     CDS             complement(18502..20526)
                     /locus_tag="ACGI57_001400"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9716481.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGSGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(20780..>21922)
                     /locus_tag="ACGI57_001401"
     CDS             complement(20780..>21922)
                     /locus_tag="ACGI57_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HFG9716482.1"
                     /translation="ISNNAVDSKLMNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQ
                     LRHAVWHGVTLADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILF
                     GFFLNYLGNPDLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTF
                     IIVGYYILAKPYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCT
                     MGCVLLKENIGEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGI
                     LLSIFYFICDIKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTL
                     VEWTTYELITPWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
BASE COUNT         6840 a         4052 c         2288 g         8742 t
ORIGIN      
        1 attataaaaa aaacagcaaa gaaatttatc ttctttgctg tttttataac gttcatcact
       61 attttaatat tactaaactc ttaaatctct atgtaaaaca ttaaatgtaa ttatatattt
      121 aatgttaata ttcaatccta tgtaaatcaa tatctattat tattgtaaaa cacgtgtagc
      181 agttataagt cttcttgcat aattagttcc tggagccatg ctagtaactt ttactacatc
      241 tcctgtttgt ggtgaatgta tgaatttaga atctcctaca tacaatccaa cgtgatttat
      301 tgaaccacct gaaccaaaga aaactaaatc tcctggtttt aattctgctc ttgatacttt
      361 ctttcctact gttgcttgtg atcttgatac tcttggtaaa ttaactcctg ctccattttt
      421 atagacataa gaagtgaacc cagaacaatc aaaagaattt ggtccagttg ctccccattt
      481 atatggctta ccttgttgct taaatgctaa gtttaaaaca ttttgtacct ttgcatctac
      541 atttccacct tgaatagtta aataatctcc atttacccat cctgtaacat tgtcaacact
      601 ttttatttta atccatccat ttgaatgtaa ttctaaaact tcaactttgt ctcctgtgtt
      661 tatctttcct actactgcat catttgcact agctccactt cttacattta aaaaatcaca
      721 ctggtctact gttgcactca taggtgaatt agttggctct gctgcactag ccgttattgc
      781 tcctgcttct actgtgacaa ctcctgctat taacaccata gctataaatt ttttcaatat
      841 atgtttttta tttttaatat tcatcataat ctcaaatacc taatcctttc gaaaatttaa
      901 cattttgttc cttcgtttcc aattcctaat taacattcta tcttacatca aaataaattg
      961 caaccttttt tgtaatcttt ttgtaatctt tttgtaatct tttgtttctt tttagtatat
     1021 atttgtttct ttttgctccc cctggttaca aattagaaat atattctact tttctctagt
     1081 tgttgcaatt tctttgatat tagcatcttc tggtttaata taagttggca ctaattccct
     1141 cataatacca tctatttttt caacctcttc attgttagta atttctctta aaagttttaa
     1201 gtattttgtt attttttctc tatcaacatc tattggtcta ccaataaaaa tctttttatg
     1261 ctctgtatcc aacagacctt cttctgacat aagtagttct tcatacaact tttctcctgg
     1321 tctaagacca gaaaattcaa tttttatatc aacatctggc tcaaatccac tgaatttaat
     1381 taagtttttt gctaaatcca atattcttac aggttctccc atatcaagta caaatatttc
     1441 tccaccttta gccatagccc cagcttgtat aactaagcct actgcttctg gtattgtcat
     1501 aaaatatcta ataatatctg gatgagtaac tgtcactggt ccgccatctt caatttgctt
     1561 cttaaaaagt ggaattacac taccattact accaagcaca tttccaaatc taacagcaac
     1621 aaattcagtc tggctttcag cattcatagt ctgtataatc atttcagcag ctcttttagt
     1681 agctcccatt atgttagtag ggtttacagc cttatctgta gatataagga caaatttctt
     1741 agctctatat tttgaagaca agccagctat gtttaaagtt ccaaatatat tattctttat
     1801 agcttcgcca gggctcgatt ccataagagg aacatgttta tgtgctgctg catgaaatac
     1861 cacttctggt ttatatttat taaatatttc atccattctt ttttcttctc ttattgatgc
     1921 tattactgtt tttaaatcta atttattttt atattttcta agcaattctt gttgtattga
     1981 gtaagcatta ttttcataat tatcaactat aataagctct ttaggattaa atcctgcaat
     2041 ctgtctgcaa agctctgaac caattgagcc tcctcctcca gttattagga ttacttttcc
     2101 ctcaatgtaa ttacttatct ctcttaaatt tgtttttata ggctctctac ccaacaagtc
     2161 ttctatctct acttctctaa tctgttttat atcaactttt ccatctataa tttcgtaaat
     2221 tcctggtatt gtctttattt tacagttagt gtttttacat atatcaataa tctctttttt
     2281 tctacgtttc tctatgtttg ctattgaaaa gattatctca tctacattgt acttttctac
     2341 cacatctttt atatctgaag ttgttcctac aattttcaca ttgtggatta ttcttccaat
     2401 tttattttta tcatcatcaa ttatagccac tgctactttt ttaagttctg gatttctttt
     2461 tagttcctgt ataaccattt ctcctgcatc tcctgcacca attataagga tatttgaaac
     2521 ctttcctcta gaatatgtct tgttcatgac tattcttata gctctatagg ctattcttgc
     2581 gccacttgta aacattataa taaatattgt atttaataca taaaacatta taggataatc
     2641 aaaccctaat aatttattta ttatataaat cggaattaaa taagctaaag ttgccacaaa
     2701 aatagatata atttcttctt ctcctgcata tctccataat gtgttgtagc acttaaacaa
     2761 tctaaatgat actatatgaa gtactatata tatacacgct gaaattatta tatcattagt
     2821 ggtaaatgca tttaaattac ctgtcaagct taaagacatt cgaaacgcta ctagtacgct
     2881 acaaatatct agaattaata gtgttaaatg tctaaattta tattttttaa aaagcctatt
     2941 aaaaaagctt tctttctcta aatctatact ttctcgcata actagaagcc ctcctttaaa
     3001 cagcaaaagc tacgcaccta agcatagcca acaacatcta tcttttgctg tattttttca
     3061 ttttattttt tatatttatc aaatatagaa tcataaattt tttctatatc attaaagttt
     3121 aaattaacct cataattttc tctaacaaat tgacgccctt tcttacccat attaagtcta
     3181 agttcatcat tttccattag ttttattata gcatttttta tttcttctgg ttgttttggt
     3241 tctacaatta agccagtttc attttcccat acagattctg gatgtcctcc tacatttgta
     3301 actataacag gtacttcaca tgcttctgat tctatagctg ccactccaaa tccctctctt
     3361 aaagaaggaa atacagttac atcaaaagaa ttaaaagtct tgcttacatt ttctggagat
     3421 attcttccta aaaactgaac atcattttct atacccaatt ctttagctaa atttattagg
     3481 ttgtccatct gacttccaga gccacctatt ttaagaatta ttttcttatc tttatattca
     3541 tctttaagca tcttaaatgc ttgtattaaa tattctattc catatttttt ttctaaactc
     3601 tttataattc ctattacaaa agcgtcttct ttttctactt ccataggctt aaacctatcc
     3661 atatctactc caaaaaaggt tacatctaca tgtttagctg tatatttatt agcctcttta
     3721 gccatatctc tactatttga aaagatatag tctgcttttc taaagttatg ttttataatt
     3781 tgtttttgta taaaaccatt attagggaaa tcataaatat cagtacccca aacagaaata
     3841 atatatggat gatacccaag tagacttcct ataaatccat aactacttgc atactgtgca
     3901 tgcaatatat ctggctttat ttcatttact agcttcttta tctgctttat tgaacctaag
     3961 tatcccgttt ttttagaaat attttcattt tttagttctt ctacattaga accaaaatta
     4021 taaattttta ttccttccat gtgacctcca tttaaagaga ttacatgaat gtcatatcct
     4081 ttatttttaa aaaaactaca ccattttaaa gtatgaggat ttgacccatc agctaaataa
     4141 caaacagtct tcataatatt taccttccta tcgaataata ctcaattcct aactcttcca
     4201 tatctttaat tttataacaa tttcttccat caaggactaa aggatttttc atcttttcct
     4261 tatacttatg tatatcataa tttaatacat ctttccattc tgtcattata aaacatatct
     4321 ctgcatcttc tatcgcatca tctacagaat cagcataaag tatagagtca ccaaatattt
     4381 tcttagtatt atttacacca attgggtcgt atacagtaac tcttgcccct ctttcgagca
     4441 aatattttat atttggtata gatggtgctt ctcttatatc atcagtgtct ggcttaaaag
     4501 tcaaacctaa aacagcaacc ttcatatctt gaaagctctc tactaactta gatgcctttc
     4561 taactaattt aagtttttga ctttcattaa cttctattgc agcttttact gtttttaatt
     4621 cataaccatt atgttcagat aaccagtgaa gagcttttgt gtcttttgga aagcaagaac
     4681 caccatatcc tattcccgcc tttaaaaact tacttcctat tctatcatca taactcattc
     4741 ctaaagctac atcttctatg ttagctcctg ctatttcaca aagatttgca atatcattca
     4801 taaatgatat tttaagagct aagaagttat ttgatgcgta ttttatcatc tcagcactat
     4861 ttctatttgt aactattata ggttgattaa aaggcttata gatttcctct aatatttctc
     4921 tagcccattc actttctaca cctataacga ttcttgaagc atgtagagta tcttttactg
     4981 ctgttccttg tgataaaaac tctggattag atgcaagttc tacttttaca tcatttttta
     5041 ggtttctatt tatataatct tctatctctt catttgtacc aattggaact gtagatttta
     5101 ttactactag agtatctttt gtcacagttt cagctatttg ctttgcaact gcctttatat
     5161 aatctaggtt ggctgaacca tcacttctct caggtgtacc tacagctatt aatattacat
     5221 ctacatcttc ataagctttt ttatagtctg tagtgtaatc tattcttcct ctgttgtaat
     5281 ttttaaccat aagttcttct aggccttctt catatatagg tgctattcca agtttcattg
     5341 atttaacttt ttgttcatct acatcaacac agactacatt ttcattgcct acctctgcta
     5401 gacaaacacc tgtaaccaaa cctacatatc ccgtcccagc tatagctatt ttcatttttt
     5461 tacctccaag tatataaaac ttatactctt tttttaatgg cattaaaaac atagaaacaa
     5521 aatacatata gactttttgg taaacttaaa ttttcaacat ttctataagt attccaagtt
     5581 cgttttaatg cttttatctt gttacttgat atagaatttc caactaatct gtaattcact
     5641 aaatcttctt gaatcccata tgcatatttt tcctttttaa gtaactgtaa ccaggttgca
     5701 gtatcttgtc ctcttcttac caaaggcatg ctgaactctc ctacaatctc cttatcaatg
     5761 accactgtag aacatcctat tatagtattt ctaagtaatc cttcatagtc aattttctta
     5821 ggagctcttg caaccttatt cgtttttgac ccatcttgtc tcatatatct ataagatgta
     5881 aaagaaaaac ctacattttc ttttaacata tattctatct gaatctctaa ttttctacta
     5941 tcccataaat catcactatc tacaaatgca ataaatcgac cttccgcatt cttaatacca
     6001 acatttctag atactgcagc tccagaattt ttttcaagct ttatatattt tattctagca
     6061 tcttcttttc gaaaactttt tataatttca cttgaattgt ccttagaaca atcatccact
     6121 aataaaagct gccaattttt ataagtttga ttttggattg actttatcgt ctctgataaa
     6181 aactcttctg aattataaac aggagtaatt atcgatacta aaggctcatt catattttta
     6241 ctctccttat atagcaacta gcagtactat accaacaagt ttacttagat actaattttc
     6301 tttgtccaat atatttgtaa tatcctaatg ggaataatgc taaatacaac aatttatcag
     6361 atacttttct taattcacca aaataatgac tatcagacaa caatgagtat ctggctaaat
     6421 ttatgtaatt ttttatccac atatatggat atctacttaa tggatatgta ttttgattta
     6481 ttaaatataa aaactctaat gctctacctc ttaatgcatt tttacttaat atattcttag
     6541 tcaaaccatc atccaaataa tctcttatta tcataggttt atttacacat ataactttat
     6601 agtatttaga catttcattc catactacac tttctggtaa aaatctcact tcttttcttt
     6661 ctggaaaagg aaattttata agctcatcag ttacaaatac tatgctttta tctcccttaa
     6721 cgccatactt aaaatatagg tctgcaaagt gacatatttt cttatcttca ggcatttctg
     6781 tccctatcaa atctccactt ggatatacac aaagtccaac aactccacta aatccttctt
     6841 tattctctaa agaatcccac tcatcattag caatttcaag agaatcttta attaaaccat
     6901 catcactatc tacaatgaaa aatagctctc catctgcatt ttttacacct agatttattg
     6961 cagtatgttt tcctccattt tctttgtata tatatcttat atttaattta ttttctgata
     7021 taaatttttc aactaactct ttagtactgt cgctacttcc atcatcaaca atcaaccatt
     7081 caaaatcatt gaaatcataa gtttgagctt ttaaatcatt gtacaaattt tcaagtaaat
     7141 gagctctgtt atacgtcgga gtaaatacag taaatttata tttatacatt aattaaatct
     7201 tcctctctgt ctaacttttt ttgattccgt ttattccata attaataaag tagtatatag
     7261 ctttgtataa aggtattttt tctatatttc tatatatatt ccaagtccac atagcagctt
     7321 ttattttatt accacttagg gaattactag attttctata taatgctaga acttcattta
     7381 ttccatgagc aatatgccct tttttcaata tcgataacca taaaacataa tcttcatgtc
     7441 ttacaccact cattcttatt tcaaaatcca atttagactt atcaatcaca acagtaagac
     7501 atcctaagat atttcctttt aataatcttc tataatctac attaggtgga acttttatta
     7561 ctttgtttaa ttttttatca ttttcatcca tcagttcata tgaagtaaat gaaattacat
     7621 agtcattttc taacataaaa tttacttgtt tttctaactt actactattc cattggtcat
     7681 cactatctaa aaaagctata aattgtcctg ttgccttact tagtgctaaa tttctagcat
     7741 tagagacacc cttattagtc tcagtcttta tacattttat tctactatcc tgttgcatat
     7801 aagatttgac tatattagga ctattatctg ttgagcaatc atcaataatt aacatttccc
     7861 attcttgata agtttggttt aatactgatt ttatggttgc ttcaataaat ttttcagaat
     7921 tatacatggg agtaattata gatactaaat tcttcttcat attaccttcc tcatcattta
     7981 taaaatattt atttaatttt acttaataca caatcatata tcctcttgca gttcttatta
     8041 tcattatatc tgaaaaactt atttctttca tttttttggt tatccataac ctcaaaatta
     8101 ttttcaataa tatcaataag tttattcacg gcttcatcat tattataagc caaagaccca
     8161 aacatttcac tttttaaatc tatatatgaa tcaacttttt ccatatattc atccaaatcg
     8221 aattggtaaa acaaaatagg tctattcata tacaaaaagt ctatggctac acttgaataa
     8281 tcagttatat ttgcagctga ttttctgatt tcatttgcta gagttacccc tttatctaaa
     8341 aacacaattc gttttcctgt tatattttct tttatatcat ctataaactc atgcataaga
     8401 tgatgcatat aaatttttac atttatatct ttctcatcta atattttatt taatttgtca
     8461 tcatttaaaa aattcattat atttttaaaa taatctgtat ccgtaaattt tcctgaacta
     8521 aattttatcc aatctctcca tgtaggagta tataatatat ctcgagtttg tgcaacttca
     8581 tttgttggta aattgtcata tcttgcattt ccaattatat aaactgagtc atctaacatt
     8641 ccccaatcat ttaccataac atttctttca aattcagaac ttgctgtaac tatattatag
     8701 cttttatata ggttatctat aatatttttg aactttttgt tcataggttt tcttttttta
     8761 aaacttatag tcccatgatt taaaaacact tttatcgttt ttttatgaaa ataattaaga
     8821 acaggtacag caaaattgta tggtgctata tctgctgatg gtgcatgtga aaatactata
     8881 gcttttgaat tgtaataata cagatagttt tctacacttc ctcttattaa ttttttgcct
     8941 ggaattttat caaatacttt actgtcttta tttactaccc aataagtttc tacatcatta
     9001 tgttctttta gcatatactc ataaaaaaac tttgaattat cattgtatat atcccctgaa
     9061 tgtccaccta taagccagat atctctacca ttaaacttat gcttattaaa tggatatata
     9121 aaaaaagcca aaagcatatt tattaaaaat ttaaccttgt ccataaacac catcctcatt
     9181 ataacttgta ctgtctaaat ccattaattc ttccttttat agtggcacta tgataatctc
     9241 tattggaact caatataaga cctcctatat ttcttacaag ttttgatacc ataacttttg
     9301 caaacttata attttttcta tagactattt cctttttgca aagcgctcca aagcctcttc
     9361 catagttata atctttttga tacttttctt tagatttaga atgttttttt gcaggatggt
     9421 aaattatatc atttccaaag tattttcctt taaaacctaa actcaataag tttaaaacat
     9481 aatctatctc ttcaccagca ccaaactctc caccaacacc caatttctca tcaaatcttg
     9541 tatagtcttt accttcaaaa tcaataaaaa aagttatgga tgttatagta tccaaaacgt
     9601 tggaacttgt gatatcacaa gttccatcat acattttctt aaatgcatca actttatttg
     9661 aatccatagt tttgcaactg tatattttat aatctttatt tttattaaag aaatttataa
     9721 ctttttctaa tgtatcattt tcgtagacac aatcatcatc agggaaacct ataatttgcc
     9781 ctacagcatt gtctatgcct acatttctgt tataactcaa tcctttttta gagcttttta
     9841 tatactttat atctaactta tcaatgtatt tatcaactat ttctttaact ttgctattat
     9901 cattttggtc tactactata agttcaaagt ttttgtaagt ttgattttct aagctgtcca
     9961 taagtaagta tatatcatca tatctattta atgttggcat tattaatgat attaacatta
    10021 ctacctccta cttttcatct tccacaaatc tatttccctt ttatcattta atatcacttc
    10081 ttttattagt aatactactg caaaactaaa tgaagcttct aataataaat tttctgcaaa
    10141 agagtatagt aaaaatataa ataaagctac taaatatgct tttttatctt tttctataaa
    10201 gctttctatt cctttataca tcaaatacat aaataatata caagcaacta ctcctagtac
    10261 actaattata tatacataag aactatctaa tggattagtt gcttttatag caggactata
    10321 tgaatttcca aaaggtttta gaaaattccc cttttcagcc aagtatacat gccagaactt
    10381 aggtctactt gctaataatc tatttaataa agtgctttta tcaaatactg taccaataat
    10441 tacactaaat aaagtaagta ttatcgataa tgtctttagg agcattggta gtccttttat
    10501 tttttttata tctatccatc ttattatttc tacaaatatt aatcctgcta atatagtgaa
    10561 aaatccagtt ctactatatg tggtttgata aacaaaaaat gcagaaccaa ataaaattat
    10621 tctatcccat ttattataat ctttatatct taagaatata tatgctgcat atatagtaac
    10681 catatgataa aatacactat ttggattacc aagtccaaaa tccattcttg taacaagcat
    10741 atctattttt tctatatatc tatagtggac ttcctgatta aatatcaatg aaggaaatag
    10801 attaaataat aatatagcta ccattaaaaa acatgctgat attattaaat aatttttaac
    10861 tataaattta tcatctattt gcttagctga tacagctaat aaaacgatta ataatatagc
    10921 tccttcccta tttaataaat aaagtaagac acaaactcca actagtacca tttctgttat
    10981 actttttctc ttgtcaaata aaaaaagtag cattagtatt cctattgaag aataaaataa
    11041 cgctgattta atcatcccta tatccatatt tgggtcaata aatgatatca tatactttct
    11101 aagtaatatg ataaacaaaa caaatatata aagatatttt ctaatcataa tatttctatc
    11161 atcatttaaa gtcttcattt tactttctcc cttttagctt cgtaaagtat tacttgcaaa
    11221 tattttacaa cacttccata tcttatttac acctgttaag tgtttgcttt ctaagaaata
    11281 atttctcttt ttagcccaac tttttcccca ccaatgtatc ccaaaagtgt tttctgttat
    11341 acattctggt gtaaagtctt ctttaaatcc aaaaggataa aaatattctt tgtcgtaaac
    11401 acgtattcct tcttcataat tatttgggtc aatcttctta taattctttt ccataagatg
    11461 agtcattatc tttggtattg taaacaatga agaagtaagt acttcttttt cataatactc
    11521 caataagtct tttaaaaaag gatgtccttt ttctacacct attatagcac catttatata
    11581 ctctctatca tcttcatacc cacagattag cctattattt tctaaaagag gagttatatc
    11641 ctttaatatt tgcatatctg tatccatata aactccacct tgttcataga gtacttttat
    11701 tcttgtataa tcagatatga atgcccaaag ttttctttta taacattctt ccaaaaactt
    11761 atttcctttt atttctttct ctatatcaaa gtttttttca ttccactcta ctatctcata
    11821 ttctggaagc ttttctttcc aagaatttat acatatattt tctatatttc cttttggacc
    11881 accaaaccat acataatgta tttttttagg aatcaaatga acacctccta catatattct
    11941 tgtctatctt cttcttttaa ctgctctact atttttttga catcttgagc tttatctttt
    12001 ctacaaacca taactgcatc atttgtagat actactatca aatcactaat acctattgtt
    12061 gatattaatt tgtcatctga atatataatt gaatttttag tatctatagt aatattttca
    12121 cctcttttga tattaccttc atcatcagtt ggatatattg cacctagaga atcccaagaa
    12181 ccaacatcat tccatccaaa atcaccagga actactataa catcattact tctttccata
    12241 ataccataat ctatagagat tgattgtata gttgggtata tttcttttat tttttccatt
    12301 tcttcttttg ttcctaagta tttacttata tcttctaatt tttcatatac ttttggcaag
    12361 tatctcttaa aatcttctag tattttagat accttccata cgaacattcc actattccat
    12421 acatactttt tagagtttac atattcttta gcaatttcat agtttggttt ttctacaaac
    12481 tccactacct catatgcaac atcttctata gtattttctc tattaaaatt tatgtatcca
    12541 tatcctgttg atggaaatgt aggagttatc ccaatagtta ctagtttatc attattttct
    12601 gctatataga tagctttttc caaaatagat ttaaattctt tttcatcttt tatataatgg
    12661 tcagctggat atacacacat aacaccatca ccatattttt tcattatgtt aaatgctgca
    12721 aaacctatag ctgctgctgt atttctggca catggctctg gcaatatatt actatccaaa
    12781 catttatctt ttactatatc ctttaaagct tccaattgct tctcattggt tactataaat
    12841 aaatcatctt tttttgctag agaatctatt ctgtttatag tttcgtttat taaagcatct
    12901 tctccactta aatttataag ctgtttaggt acttcttgtc tactaagtgg ccagaaacga
    12961 gttcctcctc ctccagccat tatgacattg tatactttca ttatttatct ccccttagct
    13021 ttttatatag aatccactac atttaatata taagatatta aattgttcaa cttaaattct
    13081 acatcttctt gattgtctga atttgctcca aagtaaaatt taattttagg ttctgtacct
    13141 gaaggtctta tagctatcca agagccatct tcaagtaaaa atttaagtac atctgactta
    13201 ggtaaatcat caacaccatc tttatagtct aaaattttat ctacttttac atcagccaca
    13261 ttatctattt taacacttct aaagtaaagc atcatctctt ttattttttt tattccatct
    13321 atgcctttta aagttactga ctttaagtcc tctttgtagt atccaaattt attatatgta
    13381 tctatcaaag cttcatacaa gttcatacct tttgaagaat agtatgctgc catttcacat
    13441 atcataagtg aagatacaac accatcttta tctctagcat gagttccaat taagtaacca
    13501 tagctttcct catatcccat tataaaactt ctatcattgc tttcttcaaa cgcttttatt
    13561 ttttctccta taaatttaaa tcctgttaaa gtatttaaac aatctacatt ataagccttt
    13621 gctatttttg cacctagttc tgatgtaact atagttttta ttattgtagg attgttttta
    13681 ggaagtttat tttcttcaac taaactctct aatatatatc taactaaaag tgaaccgact
    13741 tgatttccat ttaagactac atattcacca cttgaatctt ttacaacgat acctactcta
    13801 tcacaatcag ggtctgttcc tattatcaaa tcagtgccat tgttctttgc catttcaata
    13861 gctatattaa acactgactt ttcttcagga ttaggatact ttactgttga aaaattagaa
    13921 tctggcaatt cttgctcctt aactaccatt acattttcaa agccacattc atttaagact
    13981 tttcttactg gcttatttcc tgtaccatga ataggtgtat atattatttt taattttttc
    14041 ccgtattcat ctattatatt ttgtcttaaa acttgcttct taacagcttt tataaactca
    14101 tcatctactt cattatctaa tatagttatt aaatttttag ataaagcttc ttcaaaatct
    14161 atacttttaa tagtactgta atcatcaatc ttattaactt ctgctattat atcatttgcc
    14221 atatctatac agatttgacc accatctgag tcatatactt tatatccatt gtattcttta
    14281 ggattatgac ttgctgttat tactattccc atagcacact ttaaatatct aacagcaaaa
    14341 gaaagttctg gtgtaggctt taattcttca aaaatataag cctttacacc ataagcactt
    14401 aaagtctttg ctacttctat acaaaactcc ctagacatgt atctactatc atgtgctata
    14461 actattccct tttgcttacc ttcttctcct tgagttttca tgatataatt taaaacgccc
    14521 agtgtcgcac gtctaacagt ataaatattg attctattag ttcccgctcc tattatacct
    14581 cttaaaccac ctgttccaaa ctctaaattt ttataaaatc tatcttgtat ttctttttca
    14641 tcatctttta tacttaatag ctcatttttc gtttgttcat caaaataagg agaatttaac
    14701 cacatctcat aattattctt ataatccata ttttcatcct ttctgactct aatttaaaat
    14761 ttcaaggtaa ttatctatct tctaagtagt ttttgttttc cttttcttaa aaattcaaaa
    14821 gctaaatcca tctcttctac cttaaataaa actatcaata cagtatatac agaagctcca
    14881 cctaatactc cagctccaac tgatataatt tctttaattg ttcctgaacc cataaatgca
    14941 tatagattat tatatatcaa taaagtagct actgccatca caccagatgc taccaaactt
    15001 tttagaccag tctttataat tttatcccct ccaaaatatc catttttctt tttaagagat
    15061 ataaataata atataactgt aattatgttt gatgtactag ttgatatagc aatacctgca
    15121 tgacccaatg gtcttatcaa tattaaatta agaactatat ttattattaa agctatactt
    15181 ccatttatca ttggcgtttt tgtatctgaa agagagtaaa atgccctata taagacatct
    15241 cttactccac aagccaataa acctagtgaa tataatctaa gcgctataga tgtaaggtct
    15301 gttgataacg catcaaattt acccctttgg aataatattc taacaagagg ctctgctaat
    15361 ataatagctc ctatagatat aggaagcacc accaatataa tacaatttga agattttaca
    15421 atagtactta taaatccttc tttattatct ttaccagaca tttttgctag tttaggatat
    15481 ataactgtta ttatagatgt aacaaataat gccataacaa actcatttag cctatttgca
    15541 tagttaagag cagacaactt tccatcaccc aaagtggttg ccaataatcg gtcaacaaat
    15601 atatttaact gtccaacagc tactcctacc aacataggca atactaggtt gactaactct
    15661 tttatggact catcctttaa atttatatat ggcttatact tgtaggactt cttaaacgca
    15721 aatggcagtt gaaacaatag ctgacttgcc atagccaata gtgctcctgc tggcagtatc
    15781 catacatttt tacctgcact tagtgctatt gctgctatta ttataatatt atatgggatt
    15841 cctataagcc caggaattac aaaactgtca ttcacctgta aaaacgaact aaatatctta
    15901 cttccactca ggaataaaac tcctgaaatc attattttag taaataatat tgctgcttgg
    15961 aacttagcag ggtcgtttct aaatcctgct gcaaatactg taacaaattg ctctgtaaat
    16021 aaaagaccaa taatagctat tactatagcc attatatagc atatatttaa aacattatta
    16081 gtaaatttta aggcaccttc atctccgagt cttttctttg tctcaaagta cataggtata
    16141 taagttgtag caactgcaga gcctatgact gctatgatta aaccaggtat atttaatgtt
    16201 aatacaaata cgtctgcata catacctgtc ccatagaaat ttgcaagtac aagttctcta
    16261 aggaaaccta atacttttga aaacatagtg gctgccataa tccacaatgc tgctttagct
    16321 gtttttgaca tcttctttct ccttatctaa tttaaactct aaaagtagaa cttttaaaac
    16381 tgcttataaa cattattcta attaagtaaa tttaacctat gaaaagcttc cttataaatc
    16441 aaaaactatt tcgactcata tctaagtgcc ctaaatgtaa tcaatagtaa taatagtatt
    16501 aatactatac aagataagaa tgttagatta aatatgctct taaatagact ataatcacct
    16561 actgtatacc caacttcaac tacatcattt atattgctat ctttcaagaa tttatcttgt
    16621 gcacgactta agttatcact acaaatcagt attggcatat tttcttttgc tgatactact
    16681 cccaaagaaa tatattcgtt tacatcagat ctactaccaa agtctaatct agcagtatag
    16741 attttcttta tatctttttc atcataaaac ttctttatta aatcaacatt tgtacttgac
    16801 ttatttattt gctttattcc ttctatattt tttaagttag aatcaatctc tgcaataaac
    16861 tcttttgaat cacctaatgc atatattttt ttaagatttt tgccttttaa gaaatcaatt
    16921 tgttcctcaa acccttcatc tttagcccat attattggca ttccactttt agcggcataa
    16981 gaatatacag atatggcgtt ctctaatcct gttgttgagc taagtaaaaa tgcttcatca
    17041 aatcctactg ttctatacat ttctctagct atctttaaag atgtatcata tctgtctttc
    17101 cctcttattc tttctacttt cattcccatt ttttctatat tacgttcagc atctttagat
    17161 actgccttta aaccacctac tatcgtaacc ttctctggac ctagttcttt tagataattt
    17221 cttgttactc taccaagatt tttactctta gttacaacta ttggagcatt ttttgcatat
    17281 gctaaaggtg ttgcacttat actatctaca attgactctt catttacaag aattacttct
    17341 ttagaatcct taaatgctct tttatttaat tctgtcgaaa tctctggtat actcccttct
    17401 atcaattctc ttttgggatt tgcatctgca aatactccta tcgtactaga aatagagatt
    17461 aacaaaacta acgataatat agtagaaatt tttttaatca taagttctca ttccttttct
    17521 tttttgtatt ttttgacaat aaaaaagctc tttttaaaga gctttttcat tgattagtaa
    17581 tctaatattt taaaattatc tagctccgtc tcctgtcaat actactttaa cagttttaaa
    17641 gagtatctta aagtcaagta atatactcct attttttata tagtaaatat cttctttaag
    17701 tttttcttca ggagtgattt cgtatccacc atttacttgt gcccaacctg taagcccagg
    17761 ttttatagcg agcctgttga taaatccagg tatttcctca ttaaattgaa cagtaaagtt
    17821 cggtctctct ggtctaggac ctattaagcc catgtccccc tttaatatat taaacagctg
    17881 tggaatctca tcaattctag ttttcctaat aaactttcca accttagtta ttctaggatc
    17941 atctttttca gcccattgtg cgccaaattt ttctgcgtct gttctcatag atctcaattt
    18001 atatatatag aatcttctcc cgcattttcc tagtctttct tgcttataag ttattggtcc
    18061 tttgtcctct attttaataa gaattccaaa tattgctatt aaaggtaaac ctatcacaag
    18121 tcctattata gataaaacta aatccataat tctttggtac aagtcgaata aaactgttcc
    18181 ttttactatg ctatagtcaa tctcacttgc aatttcttta tctgcccctg gatagctttt
    18241 aattttaggt atttcacact catcattagt atatccagac atagttacct ccatattgga
    18301 ataaaaattt ttttaattct taatttcaac ttatttctat cttatttttg acttatttct
    18361 atattatact caataatgag cagattatca atacaagaaa catcattgta aacaatttga
    18421 ttctttacaa aaatatatca tctcttgtta tattttacat tataacagga gatgatatgt
    18481 tttgctttaa tattttctat tttattttac tgtatcttct attcctattc caatttgagt
    18541 tcccattgtt tttacttttt ctgcagtatt taacttatcc atatcgctct tattgtcaat
    18601 aaatgcacat tcaacaagta ttgctggata attattattt cttaacacat ataggtaatc
    18661 ttttccatta tcaagtgttc ttgtcttgat tcctctattt ttcatattaa atttttctag
    18721 tattcttttt aagatatttg atgctgcagt cttagttgtt ccaccatttt tatcttttac
    18781 tttgtaataa atttccacac cattaccaga tccattagac gcattgtaat gtatacttgt
    18841 aaataaatct ggctttatag tatttgataa tgctgttctt tctcctaaag ccattgtttt
    18901 atctgtatct ctagtcataa caacatttat accttttgaa cgtaaatatt cagtagttgc
    18961 taatgctgta ttaagagtat atttcttttc ttgtgctcca ccatttaatc cacttgttgc
    19021 ccctgaatca gaacccccat gacctgggtc tatcactaca gtttttcctg ctgctgaacc
    19081 tgaattatct ggttctgttg gtgcattgtg ttttgataaa ctagatgcta tagaatttat
    19141 aacactatct ttcattccat caccaatttg atatactgtt tctgctgttt tttctgataa
    19201 gtttgtacta tggtatgcag aaacataagt ttttggagtt ataagtattg gagcttttaa
    19261 tttagctgct aaaggtcctg ctgttattga atcaactatt atgtcagatt ttgcaactaa
    19321 cattgcactc aactctttat ctggatagaa agtcttaatt acatttgcat ttgtttcatg
    19381 tctatctgct ccagaaactc tattttttga agtaccattt tttgctattt tagatatttg
    19441 gtctataatt tttgatgata aactttgact accacctatg taataagctt cttctaaacc
    19501 ttgtgaggat aaccaattat atgtaccttg tggtacagaa tctttattag ctaatattat
    19561 aggttgttgg tcttcaccag cttttgatga aatattaaga gcatcatatt caccagcata
    19621 tccatttgct atgtatattt tattaacatc atgatactta tctatttctt ttgctataag
    19681 taaagatgtt tcatgtctat caactccacc aatacgagtc acatttacat ttacagcaga
    19741 tttaatttga gacactgcct tttgtgaaac tgaaccatca tcacctataa ttattacatc
    19801 acttggattt agacgtttta actctgcttt tgtactttct ggtatatcat ctttatttgc
    19861 aagtaatatt ggtgcgtcat atgtagatgc aagtggagta gctgtaattc catctgctgc
    19921 taattctcca ttaactatta caaccttact agacccatct ttccaacctt gtttgcttat
    19981 ctcaacagca gtttcaaatc tagtagctcc agcaagtttt tttactggtg cagaagtttt
    20041 tctcatatat tcatttactg cttttgttga atttgcatca cttattataa actcttgtgt
    20101 aacatcataa tccttaagat ttaatcccat agactctaag tcttctttgt ttaaaacttt
    20161 ctgttcatct ttaccattta tttttatgct ttgaagttcc catacattta agtattctgg
    20221 gtctttgtta tagtttatta caccttcata tgaatctgtc ttttcattgt aatttaaagt
    20281 agcttcaagt ggcatatcat aacacataaa atttaatgtt attgaatcag catttaattt
    20341 ttctttaaat tttacacttg cattagattt acttcctaac actacacttt ttctttgtaa
    20401 atttatatta atatctgaat tagattctgt atttgcagta gtattttcag ccgctaaagc
    20461 tacatttggt gccaccattg ctacagacaa cattcctgtt gccaataatt ttgttgtttt
    20521 tttcatcata tcccccctaa ttttttggtt ctttaaatct ttaaatcttt tttaatacat
    20581 cttaagcttg ttttatatta cataaattca atattatgta aatctttttt cattatagca
    20641 caaaaaatta caaattgtaa actttttgta atcttttatg gcatggtttt taaattttat
    20701 cgaggagaaa ttttgattat atttgatagt ttccgtatat ttgaacaaat caaagacttt
    20761 atcatctaaa aatattgcac taaattttaa taaatatttt tttcttgtac attatagaca
    20821 ttactataac ccagaatagg acatacaata aagaaaatat aagagaatcc caagttgtac
    20881 ctgcccaagg tgttataagt tcatatgtag tccactctac caaggtagtt ggataatcga
    20941 ctttgttcgt aagttttgga acattccaaa aaacatgact caatatttca aggcacatat
    21001 atgtaaaaat agggctactt ccaagtgcta ttattggggt aaatatttta cttttatttt
    21061 tgatgtcgca tataaaataa aatattgaca gtaatatacc ataactgcca gccattaaaa
    21121 gtacaaatga gctagaccat aatcttttat taaatggaaa atactgatta aagataaaag
    21181 cacctattag caggattatg ctcataacca gtattttaaa gaatttttta tattctccta
    21241 tgttttcttt taaaagtaca catcccatag tgcatcccaa catacctgaa gaaattgcta
    21301 ctatactagt taaaaaacca tctggttcaa attccggctt gtacaaatgt cccttgaaga
    21361 aatgtaaatc cactaactgt gcaagagaac cttctagctc aaatccataa ggttttgcta
    21421 atatgtagta cccaactatt ataaatgtag atatacatag aaaagtaatt attgtagcag
    21481 tacttccaac atttaatttt tttagtagta aatatactaa actagtcaca aaatagacta
    21541 atcccatacg ttgaagtact ccaagtattc ttacagtatc taaatcagga tttcctaggt
    21601 aatttaagaa aaatccaaat aatatcaata agatacttct cttgaatata ctaagtatta
    21661 tactcagtgt ggatttatta ttttttagtt tactatttat tgatattgga attgtaacac
    21721 ctaaagatat aacaaaaaaa ggaaatgcaa aatctgcaag tgttacacca tgccatacag
    21781 catgtcttaa ttgaggatac attctcatcc aagtcccagg attattgcag actatcatga
    21841 gggctattga tagccctctt attatatcta ttgatttaat cctactattc ataagtttag
    21901 aatctacagc attatttgaa at
//
LOCUS       DAYAOX010000047        21828 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.047, whole genome shotgun sequence.
ACCESSION   DAYAOX010000047 DAYAOX010000000
VERSION     DAYAOX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.047"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(17..1897)
                     /locus_tag="ACGI57_001402"
     CDS             complement(17..1897)
                     /locus_tag="ACGI57_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HFG9716483.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKVTINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGSGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGDDRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNDKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2107..8070)
                     /locus_tag="ACGI57_001403"
     CDS             complement(2107..8070)
                     /locus_tag="ACGI57_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HFG9716484.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSDSESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDEQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSKSTLE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELARYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRIEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDANTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8194..8279)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8451..9854)
                     /locus_tag="ACGI57_001404"
     CDS             complement(8451..9854)
                     /locus_tag="ACGI57_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HFG9716485.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(10316..12211)
                     /locus_tag="ACGI57_001405"
     CDS             complement(10316..12211)
                     /locus_tag="ACGI57_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HFG9716486.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKGGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            complement(12837..12980)
                     /gene="scfA"
                     /locus_tag="ACGI57_001406"
     CDS             complement(12837..12980)
                     /gene="scfA"
                     /locus_tag="ACGI57_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HFG9716487.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(13046..13408)
                     /locus_tag="ACGI57_001407"
     CDS             complement(13046..13408)
                     /locus_tag="ACGI57_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HFG9716488.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(13466..13759)
                     /gene="yajC"
                     /locus_tag="ACGI57_001408"
     CDS             complement(13466..13759)
                     /gene="yajC"
                     /locus_tag="ACGI57_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFG9716489.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(13893..15014)
                     /gene="tgt"
                     /locus_tag="ACGI57_001409"
     CDS             complement(13893..15014)
                     /gene="tgt"
                     /locus_tag="ACGI57_001409"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFG9716490.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(15093..15578)
                     /locus_tag="ACGI57_001410"
     CDS             complement(15093..15578)
                     /locus_tag="ACGI57_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HFG9716491.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(15590..16615)
                     /gene="queA"
                     /locus_tag="ACGI57_001411"
     CDS             complement(15590..16615)
                     /gene="queA"
                     /locus_tag="ACGI57_001411"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFG9716492.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(16933..17952)
                     /gene="ruvB"
                     /locus_tag="ACGI57_001412"
     CDS             complement(16933..17952)
                     /gene="ruvB"
                     /locus_tag="ACGI57_001412"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFG9716493.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(18059..18661)
                     /gene="ruvA"
                     /locus_tag="ACGI57_001413"
     CDS             complement(18059..18661)
                     /gene="ruvA"
                     /locus_tag="ACGI57_001413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFG9716494.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(18654..19163)
                     /gene="ruvC"
                     /locus_tag="ACGI57_001414"
     CDS             complement(18654..19163)
                     /gene="ruvC"
                     /locus_tag="ACGI57_001414"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFG9716495.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(19475..20104)
                     /locus_tag="ACGI57_001415"
     CDS             complement(19475..20104)
                     /locus_tag="ACGI57_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716496.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            20307..20630
                     /locus_tag="ACGI57_001416"
     CDS             20307..20630
                     /locus_tag="ACGI57_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HFG9716497.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNKNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            20898..21752
                     /locus_tag="ACGI57_001417"
     CDS             20898..21752
                     /locus_tag="ACGI57_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HFG9716498.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
BASE COUNT         6458 a         3939 c         2448 g         8983 t
ORIGIN      
        1 tctatttact taatatttat ttttcaatta aactcttaat cttgtctatt acagatgagg
       61 ctattcctcc acctacttca aacaactttg atgaattttt accttttaat gacgattctt
      121 gtgatttatc taacgtattt gtagcaagta taactggtga attgttttta gctgctaatg
      181 gtccaactgc taaagcatct ataagtttat cttgatttgc aactacaact ccatctagct
      241 tagattgaga gtagaattta tctattactt tggcattcgt ctcttgtcta gtttgacctg
      301 ccaccctatt gtttgatata tctttgtttg ctatttttcc aacttgttca atagccttgt
      361 tagatatttt ttctactcca ccaataaaat atatatttga caccttattt gatttaataa
      421 attgttcagc ttcattatct acaccatctt tttgtgtaag tactattgga gttttttctt
      481 taccagccag tgatgctatt gataatgaat cagcttctcc atttcctgct cctatataca
      541 acttgcttac attattcgtc ttatctattt cttttgctat attaatagaa gtttgatatc
      601 ttgtatctcc acctattctt tgtactgata tttttgaatt tacagctttg atagcttcaa
      661 caactgaatt tggcattgag ttgtttccac ctatcacaac tacttttgat ggatttagcc
      721 ttttcaattc ttctacagtt ttttgaggaa gtttatcatt tgatgataaa agaatcggtg
      781 agttttttac actagcaagt ggtgtagctg taagcccatc caccatactt ttatcttctc
      841 catttactat tacaactgta tctgattttg tccaaccact ttgagatatt ttgatagctg
      901 tctcaaatct atcatcacct tttaattttt tgactgtgac attagatgta gaatcacttg
      961 aatttcctcc tccagagctt ccaccactag atgagccgcc tgaattactt cctccaccat
     1021 tatttccacc actaccatta tctggtggta attcatccgg ttttgataat tcattttcaa
     1081 ctatgtattc taaacttttt ggaagtgtaa cttcaacata tttgtttatt gtaacttttc
     1141 ttcccttagt tgttttttgt gctaatccaa gttttaatat tacatttgaa ccactttctt
     1201 gtgtttcttt actaacaata gaatagttta tcctaatatt gtttaataaa tattcaagtg
     1261 cttcactttt ctctacctta tacttttcag ggtcattttt actagaacca gttcccccaa
     1321 tcggttttac ttcactctca ttaataaggg taaatattaa tccttcatca gagcttatat
     1381 tttcataatc cactgttttg ttgttatatg ttacaatttt taaagctggt ttatcatttg
     1441 ttccttttcc taaaacttta tagtcagcat ttgagttact tccagatttt tttacagata
     1501 ttcttcctat attcaatcta tttccgtcta gtaagtcttc atttgatgta acatatatat
     1561 ctaatgtagt tgtattactt gatttgttgt attctgctct acaagtagca tttgcatttc
     1621 cactttttat ctgagatttt atattatcag accaagtaat cttttctttc tgctcattat
     1681 ctactacttc aacctcacct tgtactttta tactggcttg aaatgaatat actatgtttt
     1741 ctactgagtc cacagatata tctatagtat ttactttctc atacgttttt gttccttgta
     1801 aaactggagc tgcatatgca catgtagcac taccagttac agctactgcc atagttgata
     1861 ctgctatagt tcttataaaa gattttcttt tactcattaa cacccgtcct tatctatcat
     1921 taactaagtg gataaattta cttatccacc tagcttttat atttaattta taaattaccc
     1981 taaatttatg attacattta tatactttac ttttaatatc tactatttta aaactattct
     2041 agtacctaat cttattagat atacaatgtt agtgttagta tttttacgtt tttagagttc
     2101 cacctactat ttatctctaa caatattacc ctttacttta ttgcttacag ttataacctt
     2161 cttattactg atatcaatag aatttcttgt agcttttcta gctttactat tactatcagt
     2221 aagacttatt gtctttaact catcataatt cttagctcct acaggtacat acattgtatc
     2281 acttacaaat ctttgaccta ctggagtatc tccttgaagg tcattgtatc tatatagttc
     2341 agccttaacc ttatcactta aagaatcaaa gttttctttt ggcatatagt aaacccatgt
     2401 acctttactg atttcaccta gttcaccttt ttctggaact tctgcaaata aaagtgcttc
     2461 tccagatata gtcttattgt ttttatctat aagtagtggt aaattaaatg ctggatttcc
     2521 actgtattca cctgtaataa ctattgaacc cgctggaatc ttacctgttt catcttcact
     2581 tgtgctagag tcatcacttt gatatacata atcttctgct aattttccaa ttccattagt
     2641 acgagtttcg tctgcattat tcattgtgcc aatttctata ttatcacctg gaggtcctat
     2701 tatatctaat tctgcaactt ctatattaga cttattattt gagattagtt ttacatatct
     2761 agcttggtat gtccaaagtt taccatcttt attaaatgta actctagctt cattatcttg
     2821 gtcttttcct ccaccaacta aatcttgtcc atattcaaaa gttccttgac ttacatcttc
     2881 ccagttcttt ttatcattac ttacttgtat tttgtaatta gttaaaggat tgtatgttgt
     2941 gctagtattc tttctaaagt taaataatcc ttttaatgag aacttcctag cttctgtctc
     3001 tggttttgta tattttagtc ctactatagg taatgtttct ttcatgtcta ctattatata
     3061 tggattttcc tctacattta tatcagggtc tttttgaggg tctttgttcc actctgcttt
     3121 acttattatc tttcctttat atgtagtatt tgcatcatta tcttttatat tatcaatttc
     3181 aggattttgt aatggtccat gtgagtcatt ttcagttcta acatcaccgt cagaaacagt
     3241 atttgttatg aatgaccatg ctgatttatt tattttccct tcatctgata attttacagt
     3301 accaacttca aattcttctg ttggatttag gtgataatca tatgcaacta ccttatatgt
     3361 aaatgctctg ttattaagag gagatatatt atcagtaaat ttagtcttac tatttccttt
     3421 tgcatccaca aatccaactg ttctgtattt tacctgtgtt ccattttcac catctaaata
     3481 atttccatca cttcttatta tttcgtatcc tagtatctta tctttatcct tatctacatc
     3541 taggtctata gatattttat tagatttaac tagagaacca ctttgtattc catcagaaaa
     3601 actagcactt actttagtat cttttgccat actagctata ttttcattta aaatctttct
     3661 tctagcttcg tcatttacat attgtatctt tcttgattct ttttcgtagt tcttactatc
     3721 cattatactg tttaattctg gtgtaatttc tactccccat gccttaaagt aatctcttaa
     3781 atctttttca acaacacttg atgccattgc tactagcatt tggtcttttg gcaaactatt
     3841 aattgggtca ctacttgaaa gtgctctgta ttttcttgaa atctttgcat agaatgtatc
     3901 attacttaaa tcagcatctg agttattctt taacattgaa cccgtaaaat catcatcata
     3961 agctagatgt aattgccata acattccaag cctttcaaat acattgcttg gtatacctaa
     4021 gctatttgaa gttacatgct catatatctt atccattgtt gaaccttcaa ttcttgaatg
     4081 tgtcttatca tcaaaggtct gagcaagtaa agcaagtaca ttgttactag tttcgctata
     4141 tgtcatcttt ccaatatctg taacatgtcc tatctcatga cctattcccc atccaaataa
     4201 gtttcctcct gtagcttttc cactatcatc tatttggaaa ggtactccat tcataagccc
     4261 tggaactgaa ccatattcta ctcctacatg taatccagaa gcatacataa atgctccaat
     4321 aaacattctt tggtatttta tgttcatacg agattttgga gccatatcat ttttagttaa
     4381 gttaaattca ttatcatcta cagttccatt accatctaaa tcttgtgttt taaatactcc
     4441 tctttctgag taaactaaat ccattatttg ttcccatgca agcatactat tatataatct
     4501 atctacttgt gtatttaaat cagcatttcc ttttgatatc ccttgatata cagctgttgc
     4561 aggaacattt aatgttactt tatcactttc aatatctgta gtgtttaaca caccagtttt
     4621 ttctgaatat ccatatgaat taccaacatt tgttcctgga taatatggtt ctccttttac
     4681 agcatcttta tatctagcta attcttctat atatgctttt atctttttct tagattcaga
     4741 aactcccgca ggactcttat catttattgt tccatttaga ttaaggtgtg gtatcttagt
     4801 agctcctgct acacgtactt ttatatctcc tccacttgga gtatcttcag cgtatcttat
     4861 gtataaactt cctccacatt ctatatctaa actatgaagt ttgcttaatt gaattacatt
     4921 ttttccagtg ttaagattta tttctccact cttatatgca ccagattctc catagtactg
     4981 tgtatatatc aacttaggag tttttccact tttacttcca acatatactg tgatttcatc
     5041 tcctgctcta gcagctactc ctaatgcctg ccagtcattt tgcataccta aattattgga
     5101 atttacagta tactgtccgt ttttatcttt atattttcct aatttattta tttcttgatt
     5161 tacagtagtt acatcagtaa gtgtggctct atcattatat aagtcttgag ctaattttaa
     5221 ttctctctca ataactgtct tatatggatg atattctcca ctagctttat ctggagtatt
     5281 taatctatct tttaattcag ttattttttc ttgagttaca tcttctttta attcaacttg
     5341 taggtcatcc ataaatagat ttcttacatc atcttctata ctgtcatact cataaaactt
     5401 aagttctgat atattagact tatatgaata ggatgaacaa acacttactt gtatcttggt
     5461 agcagttatt ggttctttta atctaaccat tacatactta ttattattta cagggtctgt
     5521 ctttgtttca aaaggaacat cccccagatt gtgtaattga ccattttcat cccaatatcg
     5581 tacctcaaag tcatttattg tcttatcaga aacatcaaaa cgaggtgtag ccataaaagt
     5641 atccatagta taagatttat caaaagttat tattggtcct ctattcgttc cctcagctgc
     5701 attatatgtt gggaatgtcc aatgtgttgt aaagtcccca tctactacat tgaatttatc
     5761 aaatggattt ttatcactat attcactttt gtcatatcct gctggataag taacatctac
     5821 aatgtgattt gttggtattc caaatccacc attctcttta ggagtattaa ttaatctata
     5881 gttaggtgtt gttggtgcat ccatgctttc agttgtacct atatatgttt tagacattcc
     5941 actagttccc aagtggttag tagctgtcat tctaacttca tatgttgtat tatccttaag
     6001 accttctatc tttgaacttg tcccacgaat aagcttatca ttgtatttta tccaagaccc
     6061 attagagcct ttttctttat agtacaagtc aaaatcttta gcttgtttat gatttttcca
     6121 gcttacatca agagatttaa atttaccttt aacatttata ccttctggtg gttctggttt
     6181 tgtgtctgga acaacttgaa attctaatat actatctggg ctaaattgta taggtttata
     6241 gtttgaatct acattttctg gtactccgtc taagttttct ggtgttgcat ctttataacc
     6301 actagaccaa tctccattta aagattgtat gctaacccta tatagtccat atgcttctat
     6361 atctctaact tctattgtat ttttactggt ttgtaatttt ttagtagata atacttttcc
     6421 cttactatca attttatcta gtataagttc gtatgctgat acatttactt cattattcca
     6481 ataaaaagat actttttcat ttccaacagc agtgctagtc gatattttat ttattttagg
     6541 tatattcatc tctggctttg gtatttcttt atatacattg tttaaaaatt caacttttga
     6601 tatttctgca agatttctat cgtttgctgt tgtagctgtt atttttatag ttatcttttt
     6661 tactgctact tgtttaccaa gatttattac tatatcacta ttttgaacat ctgcatatgt
     6721 ttctaatgtt gacttactat atgtagatgt tgtagagtta tcatctttac tgatattacc
     6781 ctcttctttt ggagtatctt catctgaatt aggtttatcc attgatgaat tttctttatc
     6841 attattttta tcttgattgg tattagagtt tacttcttgt ttatcgctat tttcctgttg
     6901 ttcatcatta ttttcatctt tattatttga gttattatca gtctggttta tattttcatc
     6961 tttattattt acttcttctt gatttacttc ttcttgttcg ttatttactt cctcttgttg
     7021 ctcctcatct tttaagtttt tcatatattc tttgttttca taaggaatct gttgaacatt
     7081 accatctttg tcagtaacct caactattcc ttttgctgga gcagtacctt ctggagcttt
     7141 tatattgatg acttccatat taagtgcttc tgataaagct atatccatta ctgctggttt
     7201 ttcctcagat atttcatcat tttttgaaga tattgtaact ccattatttt ctccactaaa
     7261 taaattttct acattaccat taatatctac atcactattt ccttttaatt caaccttact
     7321 tgtatctact ataggattgg tagatactat actttctagg ctttttgcat cttcactttg
     7381 tcctaaattt ttctgtacat aatctaagtc aacaatatct acctttccat ctctatttaa
     7441 atcaaattct cttttatttg aatctatatt ctctaaaacc ttattataat cactcttatc
     7501 tactttaccg tctccattta catcacccat taagaacaat tcataattta aaggtttatt
     7561 tttattatct acacttgctc tattgcttaa taagactctt tgtgaataac ttgatatatc
     7621 aatgtcttta atttctttgt tcttaaatcc atctccagag acttctacat tgtattttcc
     7681 aacaggtaaa ttattaaata caatattata atatgatata ttctcatctt tattttttat
     7741 ttcacttcta cttgcagata atttttttat agtatattct atattatctg tcccaacatt
     7801 tactgttcct gaatcttttt ctccaagctt tattattcca ctattgttac tatcatcttt
     7861 taatcgtaac tgcatattag tcatactttc tgaatcgcta ttttttattg gcagtgaaaa
     7921 atttaaatct acttcaagac tcccccttaa cttttctgta actgattttt caactgccct
     7981 tagcttcccc tctaatatag gttctgcaaa aacgtttagt ggtgcactac acatagatac
     8041 tgctagtaca caagatattg cctttttcat gaaataacaa tcctccctta ataaatatct
     8101 aatttttcat gaatttaaat actataagtt tcagacaata aaaaaagaca ctcaaagaga
     8161 atcttaatta taaaataaaa agactttact ctttttagca actggctgat atggctttaa
     8221 aagctttaat ccctatagtt ttgcgtcatt agatttccct aattttgcca tttaattttc
     8281 actactttct tacccatttt atactatatt taaacttttt aatacaattt ttttagatta
     8341 aattataaaa aaataacatt tatattaatt tattaatata aatgttattt tccattataa
     8401 tttaattatt tatatagctt ccttacaagc ataattttgt gtatattttc ttattcttta
     8461 tatcttccat aaaattcatg tgtagcatat agattcttat ctcctgttat agctatacca
     8521 aatcccatta tttcataccc tttatgcaac atgttagttc cttcttctgg atttgtcttc
     8581 cactcattaa aaataaagtt tcctatatca tctgcatctc tatttgtata tacaggttta
     8641 tctggaattt tatatttaat tatattttca ccttgaactg ctatatatcc aggtttaatc
     8701 tcactccaat ctaagtattt caaaaaagta gaatatgaat tttttccatt tatcgtatga
     8761 gataaagtct tcttatttgc cattaaatta gaccaatcat ttgctaaacc ctctaatctg
     8821 cttgctacag ataacgctct tactccattc tcttttctat actgctttgt aagagcatat
     8881 actttaccct ttattctctc tttaaatgct ctatcatttg gattgtaata taaaccttgt
     8941 tgatttggtg aagcttgatt tatacattgc ttgataacat tctgatttat gcctcctagt
     9001 acagtcaatt tgtctgcacc acttaaaata ctcttgttac tgccattttc aactaaaact
     9061 acaggagcat tttttgctat tgtagaggca gccaaagcgt ctgttaattg taagccttta
     9121 cttagataaa attctttact atttttataa aactcttgta taactatctt attagtttca
     9181 aatctatctg tgccacccaa tctaatagaa tttgtgctct taactaattc gtcgctgatt
     9241 atttcactag aaccaagcgc ataactcttt aagcctgtcg tgtcaaatcc cacactattt
     9301 ccatctgtaa gtattatagg tactccatct ctagcagcta ctggtgatgc acttatcgca
     9361 tctgcttctc ctgtaaatcc attggctata attactttat ctattggctt aatttctttg
     9421 attttttcag ctatttttat acttgtatca aatctatttt caccaaagat tctttgtaca
     9481 accttctttt tggatagtaa ctgattctca acatttttac ttattgttga ttctgagcct
     9541 attatataca cagtctttgc tttgtttaat ctttccatag ttgtgtttgg aatctgattt
     9601 tgttttgtca tcaaaatagg cgcatttaag gctccagcta aaccacttgc acttagacca
     9661 tctgctaagc tattatctga gttgattagt attgctgtat tgtagttttg cttatctgct
     9721 atttttgctg ctgtctcata tctgtcattc ccttttattt gctcaactga tgtcaacgca
     9781 tttaccatag gagtatttac cattactaaa aatagtgtaa gtcctatagc taatatcttt
     9841 ttactattct tcatattatt ttctaccccc aatgtacttt tcatattttt tattagtact
     9901 catacttatt tttaatcaaa agttacaatt ttgttaccat attttatata tgataccata
     9961 gttatccata caactcaaac aatataccta aagtttttat tctctataaa ttcaaaacat
    10021 tataatttat tttatttaat taagttataa gttcaaaata atttatgtta ataagatgtt
    10081 taatcattat aagaagtatc tttttataat aatttttgca atatgaattt ctcaacatta
    10141 taatcaaaaa ataaaataag gtatctcaat ctaattctaa atgagacacc ttatttttat
    10201 tattttatct tgttatttgt atattttata atatctacac tatgtcttac atattaacgc
    10261 attgtataat catatattat ctaaacaaac ttttaatatt aattcctata tcttattagt
    10321 tcttagtaaa taagtttatt ttttctgcta ttttttcaat agcgttttta gctattcctt
    10381 cacctatttg tactaactta gtagcattct taagtcttaa ctcaactact tcttcttgtg
    10441 atttagaaag ttcttctgta gttaatacta ttggagcctt tgaactagct gcaagtggtg
    10501 ctgctgtaag tgcatcaact aattgtccat ttccacctac ataaccatct tttgctacat
    10561 atgctttagt tgcacctgca tagtatttat tgattacttt agcattagta tcttttctat
    10621 tttctccagc taatctttca actttattat ttaaatctat atcaactaat ttgtcttcaa
    10681 cttcttttga tacagaattt tctccaccca ctatttctaa ttcttttcct tttaataatt
    10741 tttcagcatt tgcatctaat tcgttacctg taactattat tggagcacca aattgagcag
    10801 ctctagctga tatactcata gcatcagctt ctccatttcc acctactaca aaagcagtct
    10861 taaatgtatc ttttaattgt tttgctattt ctaaagaagt ttcaaatcta tcttctccag
    10921 aaactctctc aactttactt acacctaatt tagaaagttt ttcttcagtt tcttttgata
    10981 ctttagattc tccacctact atatatattt tcttagaaga tagattactt ccaagtactc
    11041 ttaatatttc agcttctatt tctgatggta gtgaatcttt tttagataat aatattggtg
    11101 catttttttg agaggcaagt ggtgctgatg ctaatccatc aactatagca tcttctccaa
    11161 ctaaaattat agcttctgct tctccatctt tgaatctctc tttactaact tctactgctg
    11221 ttgtaaatct tgaatcacca gataaaactt ctacttttaa ttcttttcca ccttttacat
    11281 cagttaatac tttgtgtaag tctctaacta acttatggtt tttacttgaa acacttattg
    11341 tttctggctt ttcatttgcc ttgcttaaaa ctatatcaaa gcttgatttt tctggtttat
    11401 ctttatcatc actatcttct tgttcttgag taaggattgc ttcattttca aatgttaaag
    11461 catatccatt tttaacttta ttaactattt ctcttcctaa tgtagttaat ctaattccat
    11521 caaataattc ttctgctgat atgtcttgta aatctgcgtt tttaactgtt atcgtatgta
    11581 attcttttga atctatgtca ctataactct tttcataacc ttcaaaagta ccttcttctg
    11641 atgttatcac ttttgagaaa tctaaatgtt ggtctcctac atttaatgta ataacatttg
    11701 ctgaatcttt tgctcttttt acttcaactg tatttgtaga ttttatagtt gctgttaact
    11761 tgttatcaga atcttctgct aaagttgcat tgtaagcatt aactgcatct actatttctt
    11821 gagcagtttt gtagttttca attttatagt caactacttt tccacctaac acttgatgtc
    11881 ctttatcttg aatagtaact tttaaacttt gcccttctgt taaagagttt atcttatttt
    11941 gtaattgagt tgcattagtt aagtttacat tgtctacttt tatatcgtaa actctatcat
    12001 ttacatttgc tcctgctttt gtatcttcaa acttaacttc taatgctttt ctaacctctt
    12061 cgattagttt agcagaatct ttcgcactta ctgtgtaatt tttatcaaca ttttctgtca
    12121 cagtattgtc tgcgaatatt ggtgctgctg cactcataac tgtagcacct gccattacta
    12181 ttgccatact tctctttttg ttgcttaaca tatttatgtc ctccgaatat tattttttat
    12241 tttttatatt cgtatttccc acacataaat aatattatat ataatattat ttttcaatat
    12301 attttatgtg tttacataat ttactaacac aaacactaat aatatcttct tgaacccctt
    12361 ccccctgctt agctctctat aattatgctc aaagatagaa atcctatttt atagtgattt
    12421 tctactaaac aatcatatac taacaaaagt attcaaactc catttttata tactactatg
    12481 cactaatttt tgctttactc ttattactta aatatatatc actaaattaa tatctatctc
    12541 catatattaa aatatataac atattataaa aatcatcata aattagagtt taaaattaaa
    12601 aattattcta atacatgtaa ttgataaaaa ataaaatata agagtagcta ctttaaaata
    12661 aatgctctta tatgattaga tatttttgca taattaaacg ataagaaatc aaatttaatt
    12721 ttacataaat agaatatata tttttatata ataattgaaa ataaaagagg atacctcata
    12781 atgataaaag aatcactttg agataccctc ttacaaacaa ctaaatcaat aattttctat
    12841 ctttcacact cttgatttgc aactgtacaa gaagttttac aagctgattg acaagaagtt
    12901 tgacactctc cacatccacc ttttgcagca ctttgcttta aagtagcttg agataaagtt
    12961 tttacatgtt tattttccat tttcaacgcc tccatttcta attgtttcct attgattata
    13021 tcataaaact actttaatat aaactctatt taaagttgac acccaatacg cctccaatgc
    13081 ctcctgctgc tgatattaac aataacttat aaatcatccc tacttcaaat acaaactttt
    13141 cttgtgctaa aaacacagtc aaaaaaatac atactatgta caaaagtcct actaaaagcc
    13201 catacataag tcctttttct ttcatgtgtt ttgaagtgta aaaccctcct atagctgcag
    13261 aaatagtagt tataaatgat gagaccatag ttatattatc tccaccaata tctgtaaatg
    13321 tcaaaaataa gttatacact aataaaacag ccaaagttat tatatatgca tatcctagac
    13381 ctttaaaaat ataattagat tttcccataa aaaaacctcc tctcaaatta atatatattc
    13441 gagaaaaggc ttttaaatta ctaatttagt cttcttctat ttcttctttt ttagatttaa
    13501 cagttcctat agcccatttt tcaaatggaa ctttagttct atttggtcct aattctaata
    13561 ttacaacatc atcttcaact ttagctacat tagctactat tccacctatt gtaatgacct
    13621 tgtctcctac acttaggcta cttctcattt cttttaattg tttgtctttt ttcttttgag
    13681 gtctaatcat taggaaataa aagattgcga ttactactac ccatagacct atactcataa
    13741 ttatttgttg ctgtggcatt aatttttcac tcctttgtaa attatactct tttgtaaatc
    13801 ataataaatg tttatataac aatttacttt ctctatcttt gagtactatt gaatattata
    13861 tattaaaatg attataatta atagtgcaaa acctatattt catatccata ttttgcaaaa
    13921 aattcatttc taaaatcaag taatctatct tccattatcg cttgtcttat ttgtttcatt
    13981 aaatttaata aaaagtgaag attatgagta gttattaatc ttgcacccag tatttcattt
    14041 gcttttataa ggtgtcttat ataagctctt gaatagtttt tacaagcata acaatcacat
    14101 tcagggtcaa gtggagtaaa gtcttcagca taagttgcat ttctaactac tactttacct
    14161 tggctagtca ttgcagttcc atttctagcg atacgagtag gtaggacaca atcaaacatg
    14221 tctactcctc ttataacacc ttctactaag tcatctggac taccaactcc cattaggtat
    14281 cttggtttat cctttggcat aagtggagtt gtatgctcta acacgtcata cataagtggt
    14341 tttggttctc caacactaag tccaccaata gcatatcctg gtaaatcaat attagttatt
    14401 tctttcgctg attgttccct taagtcttta tacatcccac cttgtattat accaaataaa
    14461 gcttgcttgt cggtattatt atgggcatct ttacatcttt ttaaccatct tgttgttctt
    14521 tctaaagaat tttttacata ttctctatct gatgggtatg gtgcacactc atcaaatgcc
    14581 atcattatgt ctgaacccaa tgcattttgt atttccatag ctttttctgg agttagaaaa
    14641 tgttttgaac catctaagtg tgacctaaat tctacacctt cttcttttat ctttcttaaa
    14701 ggccctagac taaatacctg gaaccctcca ctatctgtaa gtattggttt atcccaattc
    14761 ataaacttat gaagaccacc agctcttttt ataagttcat gacctggtct catatataaa
    14821 tggtatgtgt tactaagtat tatttgtgaa ccaatctctt ttaattcttc tggtgtcata
    14881 gattttacag ttgcttgagt tccaactggc atgaatattg gtgtttcaat aatcccatgt
    14941 ggagtgtgta atcttcctaa ccttgcacca ctctgcttac aagtctttat caattcgtat
    15001 cttactgcgt acataaagtt ctccttaatc atatttattt ctcattattt cttaagatat
    15061 atttgtaata agctattcat ataatttatt gtctaaatag atttatcttc gtcttctttg
    15121 ttctgttgtt ccttaattgc ttcttcatca tgaatactca ttatattaag agcactcttg
    15181 tcttttgtta ctaaaaactt ttttatagca aatagtcctg ctatagctat aactcctatt
    15241 aaaacatcta tcataccttc atttttaggc agtagcaaca tttttctagc aattgcgtaa
    15301 agtaatgcct caataaatgc tcctggtata tgaagagata gcattactac aagttcaaca
    15361 cctattacta aaagtaatat ttgtgctaaa aaatcattaa gctgactgta ttgtactgga
    15421 ttttgaaaat caactatata tgctccccac ataagtctta gaacatctat agttcctaaa
    15481 aatactgcta ttagaactac tactgctaaa acacgttcaa atataaaagc aatctttaat
    15541 atagggttat tctttttttg tttttgtaaa ctatgcatct attacctccc tactttataa
    15601 tcatagcatc tccaaaactg aagaatctat atctttcttt tactgcctca ttatatgcat
    15661 ttaaaacatt atcctttcca caaatagcac ttactaacat tattagtgta gactctggta
    15721 agtgaaaatt agtaataagt ttatctaaaa ctttaaattt ataacctgga tatataaata
    15781 tttctgtcca tccactagtt tctttcatct ttccatcctc attacaagct gattctatag
    15841 ttctacaact agtagtacca acacatatta cattttttcc attttccttt gctctattta
    15901 ttttgtcagc tgcttcttgg ctaaccatat aatattctga atgcatcttg tgatttaaaa
    15961 catcctctac ttttactggt ctaaaagttc ctaagccaac atgtagagtt acaaaggcta
    16021 tatctacacc tttttctttt attttattta acaattcttc tgtaaaatgt aatcctgctg
    16081 taggtgctgc tgctgaacca ttatgctttg aataaacagt ctggtatctt tctttttcat
    16141 ctagtctagc agtgatatat ggaggtagtg gcatatttcc aagttcatcc aatatttctt
    16201 caaaaattcc atcataatgg aattttataa ttcttgaacc ttcatctgat aaatctacaa
    16261 cttctccaat tagctttcca tttccaaaag aaaatttagt ccctactttt gctctttttc
    16321 ctggttttac taaagcctgc caagtatctt cctctgttct ttttaataat aaaaactcta
    16381 ttttacctcc agtttcaagc ttctctccta ttaatctagc tggtataact ctagtattat
    16441 ttaatactaa gcaatctcct ggatttaaat attctattat atctctaaat actttatgtt
    16501 caatattacc tgtctcttta tctaatacca tcaatcttga gctagctcta tcttctatag
    16561 gaacttgagc tataagttcc tgtggtaagt caaatttaaa atcacttgtt ttcaatgtta
    16621 aacttcctct cttaaaattt ctccagctac aataatatca aaaaaacaac ttattttcca
    16681 caatattacc ataatctatc aagaaattgc tatttttaac agacaccata tccacaatat
    16741 atagatattt ttccacgata tacatgcctt atccacaata tatagataca atacacacaa
    16801 tttttatcgt gtttatccac agtatctaca gatataatta ccattttgtg gatatgtttg
    16861 aatttttagc cattttagaa atattttaat ttaatgactc ttttatttta taaatactcg
    16921 tataatctat ttttattttt cattgggata tggaattttt aagtgttcat atgctaatgg
    16981 catagctact cttcctctag gacctctatt tataaatcct aattgaagca aatatggctc
    17041 atatacatct tctattgtat ttctatcttc tccgatagaa gctgccaatg tatctaaacc
    17101 cactggtcct cctctaaatt tttctattat agtcataaga agcttctcat ctacaaaatc
    17161 gagacccaaa ctatcgactc ctaaaagttc cagagcatct tttgccactt tatcagttat
    17221 ttttccatca gctctgactt gtgcaaaatc tctaactctt tttagtaatc tatttgctat
    17281 ccttggagtc cctcttgaac gttttgcaag ttctaatgct ccattagact gtatctctgc
    17341 atctaagatg cttgaagacc taagtactat ttttgaaagt tcatcaactg tataatagtc
    17401 caatttacaa ataacaccaa atctatctct aagtggattt gtaagcatac cagctcttgt
    17461 agtagctcca ataagtgtaa atttaggaag gtcaagtctt atgcttctag cagatggccc
    17521 tttccctatt attatatcta ggcaaaaatc ctccattgct ggatataaaa cttcttcaac
    17581 acttctattg attctatgaa tctcatctat aaataaaaca tcattttcat ttaaattcgt
    17641 aagtatggct gccaaatctc cagctctttc tattgcaggt cctgacgtaa ttcttaaatt
    17701 tacccccatc tcattggcta ttatacttgc tagtgtagtt tttccaagac ctggtggtcc
    17761 atataaaagc acatggtcta actgctcatt tctcgattta gctgcttcta taaatatact
    17821 aagttgttct ttggattttt cttgacccaa ataatcttct aaagtttttg gtcttaaact
    17881 gttttctata tctatatctt ccatcttcat tgtagaagtt attatcctat tttcatcttc
    17941 aaaaccttgc atttataccc ctcctctcgt acttttgaaa atctacttta tatattttca
    18001 aatttacata aactccttaa atattgtttt aaaatccaaa tattataaaa tccaaatact
    18061 atttcaagga tttactcatt atatatgtaa gtgctttctt tataatagct tctgtattca
    18121 taccatcttt tttacatttt tgaactgcct cttttgattc cgacaaagta tagcctaaag
    18181 ctacaagtgc atctacagac tcatcttgtg atatcaaggt aggtttttgt gataataacg
    18241 ttggttcaaa ctctatgtta tttttgtcta ccttatcctt aagctctaag acgattcttt
    18301 ctgctgtctt tttaccaaca ccaggagctt tggataattt tccaatgtct tcacttaaaa
    18361 tatatgctcc taactgtgct ggtgatgcaa atgatagtat tgaaagcgcc accttaggtc
    18421 ctattttaga tacagatgtt agtaattcaa acatcttaag ttcttcccta cttacaaaac
    18481 cacaaagact catatcatct tctcttacaa ttaatttagt atatattttt gtggcttctc
    18541 ccacttgtaa attcattatt gtattggatg atacatttat tttatatcct atcccattat
    18601 tttcaacaac tatactgtct atataaattt cttctactgt accttttata taactataca
    18661 tacttaccac ctatattctt tagagttttt tcaagtttgt ttgaatgagc atggcaaata
    18721 gccacagcta aagcatctgc aacatcatct ggttttggaa ctttttttag acttaaaaat
    18781 gaagttacca tctgttgaac ttgtgcctta tctgctctcc catatccaac tacaccttgt
    18841 tttacttgaa gtggagtgta ttcatataca ggcttcccat tatgagcaca tgcaagcata
    18901 gtaacacctc tagcttgtgc aactgttata gctgttttta cattcttgtt aaagaataat
    18961 tcttccattc caacttcatc tatattgtaa ttctttacta ttgtatcaat tcctttatac
    19021 acaagttcca atcttctcga tatattcata tgggcaggtg ttgtaactgc tccataatcg
    19081 attgctttaa acttgctatt tttgtattca attataccat atccaactat ggctatacct
    19141 gggtcaatcc ccaatattat cattttttaa aattccccct aaaaataaaa gaaataccaa
    19201 aatgtatttc tcataaaaac ttatgttcta aatcattata tcataaataa tattaacaaa
    19261 tcataaaatg ctctatgtaa aatataaaat caaataaaaa agttatatca aagcaagatt
    19321 ttacctcaat ataactttta tatttattac aaataaatta ttgtattttt ataggaatta
    19381 aatgtgacta caattttaat actaatctat ttaatattaa attatttatt attaatttac
    19441 ttagtattta acttaaaata atattaattt actattaact tgcaaagtct tctaatcttg
    19501 aataggcctc ttcttcagta tccataacta cacctttttt gatttcatct tgaactgttt
    19561 taggaagtga atctactctt atttttgttt tttctactaa tgttttactt ccgctatcat
    19621 catatttata tagtgctata tttccattat cattttcaat agtatattta ccttttatag
    19681 taacatccat tgcttgtaca tctttattat cactatattt agattctacc aatatgattt
    19741 catttttatc catactcttt atttttttag ttggatattc ttttttaaaa tatgatacta
    19801 tttctgactt cgtcttatct aataatgact ctggaactat tcctgccatt gtaagtgttc
    19861 tcttatagtt agtttcattt tcgccttcac tttgaagcca aacttcacaa tttttattta
    19921 atccaaaagc ttcttttgac ttttcattca tattcgcact agtacttaca acttcaccag
    19981 tttgaactcc attgtgtttt aatcttgtcc ctatataaac tccgctactt aatacaaata
    20041 ctccaaccag tattaaggca gatattttcc aaggaaataa ttcttttttc ttcttttcaa
    20101 acattgatta cgcctccttt ttctattatt ttctccaagc tgtaagtttt tatacaattt
    20161 aggtaaattt tagaagaaaa tatatttata tttcaaatta ctctaagaat agatattttc
    20221 taaaaggtag ttttctctta atggttatat tcttgtaatt atatggacat aatttataat
    20281 aaataatcgt ttaggaggta ataactttgg aaagaataaa ctgtaatgta attaactgtt
    20341 cacataacaa cagccatata tgttatgcag ataaaatttt tataaatggt gaaaaagctc
    20401 acaatgacag acatacttgt tgtagttcat ttttagatga atctatttat agtaatttat
    20461 ctagcaacgt taataataaa aattgtgagg aattagtttg tactgtaaaa aattgtaaat
    20521 acaacaaaga tggtagctta tgtacacttg ataatatatt ggttgctcct gaaactgata
    20581 gagtaaacct ttatattgaa acatgttgct ctagtttcga atgtaaatag tcatagtata
    20641 aatataaggg tgtcaaatgt aattaaacta acatagtgta gagtatctac aactattagt
    20701 ttgattatac ttgacaccct tatttatttt aaataaaatt tcattattat aaccatgttt
    20761 ataatgttct aactgtaatt aaatttgata aatttctata aattggtata atcaatatgt
    20821 aaattgaatt tagtaaatta atcataaaaa taaaatatcg agtatatagt gttatactaa
    20881 ctagaaatga ggtgcttatg agtacatttt atattcaatt tctttatttt gtaatttata
    20941 gctttatagg atggtgttgt gaaactacat attgttcagt tttacaaaaa caatttgtaa
    21001 atagaggttt tttaaatggt ccattttgcc ctatatatgg atttggagca ttagcaatag
    21061 ttgctacctt gactcctttt gtacacaata ttcctttgtt atttttgttt agtataatta
    21121 taactagcat aatggagtac tgtacgagct ttattttaga aaaaatattt aatatgactt
    21181 ggtgggatta ttctaaacat aaatttaata ttcatggaag agtttgtctg gaaaactcac
    21241 ttatgtttgg tgtattatca ttgattgtaa tgcttatagt acatcctatt gtggttgact
    21301 ttattaactc tatctctaaa aatgtgctat ttatatttgc tatatctata gaaatttatt
    21361 ttgttttaga tttagttatc acagtccata caattttaca attaaatggt aagttaaaac
    21421 aaatcaatct tataattaag gaattaaaag ataagaaaga atactacaaa cttattacac
    21481 aagaaactat agaaaacaaa cttgaatctt tagttgaaaa tagactggat actttggaag
    21541 ataggtttga aatacttaaa gaaaaagaaa attatcgcta tgctaaaaat cgtattgatg
    21601 agctaaaaaa taaacttaat actttgctgt ctaaccacaa attacttcat agacgtataa
    21661 tcaaagcatt ccccaacata agctctaata agcatacaga tattttaaat aaaatcaaag
    21721 aaaatattaa atcaattaaa aaagataaat aataaaataa acacataagt taaataataa
    21781 aaatactaat acataagatt aaggtttaga atattttttg taaaatac
//
LOCUS       DAYAOX010000048        21309 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.048, whole genome shotgun sequence.
ACCESSION   DAYAOX010000048 DAYAOX010000000
VERSION     DAYAOX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.048"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            236..1210
                     /locus_tag="ACGI57_001418"
     CDS             236..1210
                     /locus_tag="ACGI57_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFG9716499.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1326..2387)
                     /locus_tag="ACGI57_001419"
     CDS             complement(1326..2387)
                     /locus_tag="ACGI57_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFG9716500.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(2999..3850)
                     /locus_tag="ACGI57_001420"
     CDS             complement(2999..3850)
                     /locus_tag="ACGI57_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9716501.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(3867..4787)
                     /locus_tag="ACGI57_001421"
     CDS             complement(3867..4787)
                     /locus_tag="ACGI57_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFG9716502.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIKGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(4897..5811)
                     /gene="murQ"
                     /locus_tag="ACGI57_001422"
     CDS             complement(4897..5811)
                     /gene="murQ"
                     /locus_tag="ACGI57_001422"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFG9716503.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(5910..7007)
                     /locus_tag="ACGI57_001423"
     CDS             complement(5910..7007)
                     /locus_tag="ACGI57_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFG9716504.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(7240..8586)
                     /locus_tag="ACGI57_001424"
     CDS             complement(7240..8586)
                     /locus_tag="ACGI57_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFG9716505.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(8675..8983)
                     /locus_tag="ACGI57_001425"
     CDS             complement(8675..8983)
                     /locus_tag="ACGI57_001425"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9716506.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(9246..9644)
                     /locus_tag="ACGI57_001426"
     CDS             complement(9246..9644)
                     /locus_tag="ACGI57_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716507.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(9646..10797)
                     /gene="anmK"
                     /locus_tag="ACGI57_001427"
     CDS             complement(9646..10797)
                     /gene="anmK"
                     /locus_tag="ACGI57_001427"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase AnmK"
                     /protein_id="HFG9716508.1"
                     /translation="MYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEIPKD
                     IKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHGQTI
                     FHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEFLLY
                     SDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYDKNG
                     YFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDLIAT
                     VTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQDEYG
                     YSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(11237..12256)
                     /locus_tag="ACGI57_001428"
     CDS             complement(11237..12256)
                     /locus_tag="ACGI57_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFG9716509.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLIKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(12250..12930)
                     /locus_tag="ACGI57_001429"
     CDS             complement(12250..12930)
                     /locus_tag="ACGI57_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9716510.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GVGYIIQC"
     gene            complement(13082..14989)
                     /locus_tag="ACGI57_001430"
     CDS             complement(13082..14989)
                     /locus_tag="ACGI57_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9716511.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAMFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(14990..15742)
                     /locus_tag="ACGI57_001431"
     CDS             complement(14990..15742)
                     /locus_tag="ACGI57_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716512.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(16058..17212)
                     /locus_tag="ACGI57_001432"
     CDS             complement(16058..17212)
                     /locus_tag="ACGI57_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9716513.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(17215..17682)
                     /locus_tag="ACGI57_001433"
     CDS             complement(17215..17682)
                     /locus_tag="ACGI57_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFG9716514.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(17696..18184)
                     /locus_tag="ACGI57_001434"
     CDS             complement(17696..18184)
                     /locus_tag="ACGI57_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716515.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(18198..19529)
                     /locus_tag="ACGI57_001435"
     CDS             complement(18198..19529)
                     /locus_tag="ACGI57_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFG9716516.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(19543..21108)
                     /locus_tag="ACGI57_001436"
     CDS             complement(19543..21108)
                     /locus_tag="ACGI57_001436"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucoside-specific PTS transporter subunit
                     IIBC"
                     /protein_id="HFG9716517.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
BASE COUNT         7064 a         3709 c         2266 g         8270 t
ORIGIN      
        1 ttcatttgct atttaaaatc aattttaagt tttcttctta taataaactt cctctttgtg
       61 gtctcatgtt agtctctaaa tttaaaattt tacaaaaaat tttcataaaa taaaattata
      121 ttgattgtat ctaaacaaat agggcaataa aataattaag tatagttact tacctttcaa
      181 gcaatattag ttttatgtta gaattttatt atattgattt acaggaggtt tgcatttgaa
      241 taatattaat tcacttaaag taataaaatt atctcttgga tgttctcttg ctatatttat
      301 tgcttggcta ttaaaattag aatattcaat ggttgctgga gttatagtac tacttacagt
      361 aaaggacact aaaagagaaa ctcttaaagg ctccattgga aagatttatg gttttctttt
      421 atgtactatt ttttcatatt tatgttttaa tattttagga tataatttga cttcattttc
      481 aatatttata tttataatta taccactttg ttttttgtta aatattcaag atgttattgc
      541 tatgtgtgta gtcatagcat cccattacta ccttcaagga gaaacttcta tcaaatggat
      601 tttaaatgaa gctggaatat ttgcagtagg aactggaata ggtgtcttaa taaatatgta
      661 tattccaaca aatatacata aaatccatga agttcaaaaa aagttacagg aagaagtgag
      721 tattgtactt atagacattg cagatattat tgtaaatcct aaaaaagaaa atggatatag
      781 tagagattta tatacattaa attcacttat tgacagcagt atttctgaaa cttacgataa
      841 tattaataat actttattaa gtgatactag attcttctta gagcatatgg atattataaa
      901 aagtcaacgg gatatcttag aaaatttgta tagttatgta tctcaactaa attctacacc
      961 accacaagct catattctat ctgcttttat acataagata ggttatactg aatttgaagc
     1021 tgaaacaggc aatcttcttc ttgaagaatt aaagagacta atgcttagta tgaaaaacca
     1081 gcctctacct gttgacagaa cagagtttga aaatagagct attttattcc tatgcttgac
     1141 tgagttaaaa cagtttttag ttaatcgtaa acatgctcag atgcttagag ataataattt
     1201 ttataaataa aaaggggaga aatctagcag ttgacctgac tacaaaatat cagatataat
     1261 attaaatgcg cccctagtaa gagggcgcaa tgtttataac ttattctact gtcatgatta
     1321 aatcattatt catatcgaac atacattatt tgaccaagtc caatattagc tttttcatca
     1381 gtgatttcta ttttaatagt cacatcattt tttttacagt acttcgctaa ttctgcacca
     1441 gaacgatttt taccactaaa atctaaagaa aatggcttat catatttaaa aggatattca
     1501 acaggtttac cctctggtgc taatgcaaac atcccatatg gtgtgttcat aaatcctatt
     1561 acagcaatcg taagatttga tgggtctaaa tttgattcac tacctgtaaa tgtaattgta
     1621 tttactgatg aattaactac actacatcga acaccttcat acattacatg tttttcattt
     1681 atatcttctc cttttttccc aaaatttcca acagcctttg ccccttcttg atgtggtaac
     1741 atagtgtgct tcccaggtgg tgctggtttc tgtgtctgtt ctggttttac cgtctgtact
     1801 attgcaccaa cttttggata tgctgtctta tcaaatggta atgggtggct aagactcttg
     1861 tatatgatat cctttcctgc tgatttatta gcatcaatat ctacatgata caaacgccca
     1921 tcaccaaaac ggataagatt ccactggtgg tcatttgatg tgctgtgtac ataataagat
     1981 tcaattccca tagcatcaca caaagctttc atgctcctag ccattcctga gcatgcacct
     2041 tttttagtaa ccaatgtata ggcagtattt gctccctcta ctgcattcca gtcaacatat
     2101 gttgtgttat ttttaatgtg attgtaggca actaaaatct tagcatctgt ggtcatattc
     2161 tttgtgattt tcttgttcat aaaatctgct gttgcttgtg ctactgagtt tagttgggct
     2221 tgtgacagct ttggaccata tacgctattg gttccagtga catagctttt tggtgttttt
     2281 gctgttacct ctttggctgt cataccataa actgtcatac tcatacctga gattaaaact
     2341 attgttgtaa tcaataactt ggtagctaac ttcctaattt tcttcatact taatattctc
     2401 ctttataaaa cattgttact tcctattctc taccattaat aaaattaatt taggatttaa
     2461 aaaggtggat tgtcaacatg tatatttctc cattcataca ttcgtgaaat atttaaattt
     2521 catggaaaat ctaatagata ttttctagat aatttgtata caatacaact cgcaaatcag
     2581 ggtgtttgct gttagtttga gaaaatgatg atgattaata cattcggaat ctttgaccta
     2641 taataaatac cattgtcttc aatcattaat aatggtaaga gtaaagagaa ttatcaataa
     2701 tacagttaaa atcaactttg tatacatttc atacattctc tcccaacaac attatacttc
     2761 ttataatttg tatattcaat acgttttctg ggaaaatata ggatatatct ataaaaatat
     2821 aaaaaaggag ttgaacttaa aatttgatta aaaaaaatca attttaagac aactcatttg
     2881 tattataaaa atgctatata ttattattaa gtaagtcaat attttagata tttatctatt
     2941 tcttattgac ttacgcttat agtctaactt atttgtttag ttgatatata acctagtttt
     3001 aagcattatt aatatttaaa taatcaagta tcctttttgt ttcttgtagt ttctgttctg
     3061 cctcatttaa gttttcttga attatagaaa tatatattat atctatcaac gtaagcattg
     3121 ctattcttga agaaatagct ccttctctaa gccttttttc tatatttggt actactagtt
     3181 ttaaatcact aatcttacta agatgattgt cactatattt agttatacaa atgaccttac
     3241 atccttttaa ttttgcattt tctatagact ttattacttc ttttgattct ccagagtaag
     3301 atattgctat agctatatct tctttatcta catttgaaga taccataagt tgtgtatgac
     3361 tatctaaaga ggtaaatgcc tgtttattta tccttaaaag tttcatctgt aaatctagtg
     3421 caactaaagc cgaaccacct actccaaata tatatacatt tttagccttt attatctgtt
     3481 caactgcttc ttttattata tctttttcat tcaacaacct tgtttggttg atagtctcta
     3541 tgtttttgtt tgtaattttg tttataacac tatctatatt acaatcatag ttttcatcta
     3601 catatgtgta ttcattttct ttgcccatca aatctttagc tatctcaatt tttaaatccc
     3661 caaaacccga atatcccatt tttctagaaa acctaactat acttgctgta cttgtttttg
     3721 agtttgacgc caattctgat acagaaagct tacttatagc ctctgtatta ttaagtatgt
     3781 aatcagctat ttttttatct acttccgtta aatcttcata tatctgtttt aattttaaaa
     3841 tgcaactcat actctatcac ctctacctat ttactataat atttcttctt agcaagtaaa
     3901 catgctccta aatctggagt caacactggt ctcactatac caaatctcgt tttattcaaa
     3961 tattttttaa gtggctctaa tattaaatct cctgacttaa acactccacc tgaataagat
     4021 acctttatct ttgtaccttg ttcaaaatga tgctctaatc ctattataag cctacttaat
     4081 tcataagctg catcatcaaa tattttacta gcagtattat caccttcaat aactgctttt
     4141 aaacatatcg tagcaaactt tgctatttct attctatctc cttttatttc atcatttaca
     4201 tattttatta tgtcataatc attctctata ttaagatgtc tttttattaa atcatataat
     4261 tttgtttttg aacaccttcc atcagactgc attgtgaaca tttttaaggt agccattcct
     4321 atataatacg ctgaaccttc atctcctaat acatagcccc atcctccaca tctgtgaaga
     4381 ttcccatctt tatctaaagc ctgtgctatt gaaccagtac ctgcaattat atttatacct
     4441 ttttccccat ttaaagaacc tgctaatgct atttctacat cattacctag agtataattt
     4501 atatgactat atgcattttt tgttgctact tctaatgcat acaatacttc ttttattttt
     4561 ccatatccag caagtccaac aaatgcatac gttatttctt ctacatttat cttagcatct
     4621 ttacaaactt cttccaaccc agttattaat aatttcgtta gattatcaaa tcctatttga
     4681 ttataatgac aagttccttt ggttatggtt cctactatat ttaattcttc atctactaat
     4741 gtaaatgttg ttttcgttcc tccaccatct acacctaaaa aatacatact atcctcctac
     4801 aatatcttta ataattcttt caatacttaa tatctaaaaa taaccttcca atgctgtttt
     4861 tttaatattg aaaggttatc agtactatta tttattttac aatttttcta ttgattttaa
     4921 agcttcactt atataacctt cttgtctatc taggatactc tttgcactat ccttatctaa
     4981 accacttttt atcataaata tagccaattt aacgctatta tctgcttctt ctaagaattt
     5041 ttctgcaaca tcatatttta catttgtagc tatcattatt attctttttg ctctttcaac
     5101 caacttttca tttgatgtct taacatctac catcaagttt gagtatactt ttcccatctt
     5161 aaccatagaa gctgttgtta acatattaca taccattttt tgagctgtac cacttttcat
     5221 tctagttgaa cccattatta cttctgcccc tactataatg tttattggta aatccgcttt
     5281 tttactcatt tcactattcg gattcatagt tattgatagt actgcacacc ccaattcctt
     5341 agcatactcc attgcaccta taacataagg agtccttcct gatgcagcta taccacatat
     5401 aacatcctta gatgtaactc ctctatctat tatatcttgc tttccaagtt ctttatcatc
     5461 ttcagcacct tctattgctt taaatattgc ttgatttcca ccagctatta tagcttgaac
     5521 aagctcaaaa tccactccgt aagttggagg acattctgtg gcatctagta ttcctatcct
     5581 cccactggta cctgctccta tatagaataa tctacctccc tgtttcattc tatctactat
     5641 tatattaaca gcttttgcta tactctcctt ttgttcttca acagcatagg ctacagtttt
     5701 atcttcgtta tttattaatt ctattatgcc taatgtatct tctttatcta tttgcaatgt
     5761 attcttattt ctaccttctg taactaaatt ttctaaagtt tttattaaca tatcatttcc
     5821 tcattttctt aatattaaat ttttaaacac attttaattc caactttcat aattatattt
     5881 ttataataaa ttaatatttt cataataaat tattttgttg agggtttaaa attaaacttt
     5941 tgccatggtt taatataatc taatataaat aattcttctt ttcttatata tcctactaca
     6001 ttcgatttac cactgttttt catatcagtc aaggctattt gtaactcccc tgcataatga
     6061 ccatattcac tactttcaat tactatatct cctcttttta ttattttagg agtattaaat
     6121 agttcaaatt tatatccttt atatttaaca cgagattgag tagaccttat cagactatca
     6181 gatatgtccc ctctattaaa atgtatttct tcaaagagta tcttcttctc aacttctcgt
     6241 atattttcct ctattgctac atcaaatgta accatatctt ttctcataga acctaatacc
     6301 tttagctcct catatgttgg ataacaattt gatattatta tatcatttat attattcata
     6361 gctattatat gctttacttg aatatctata ggtagatttc tgtgcatctc aattgttgga
     6421 agcccatcag ttacaggcca tggtccaaat gtagtttcat tttgacttgt tacaaaaact
     6481 gctgttctta atccatactt attaaatctt tctgtacatt ttataaagtg ttctaaattt
     6541 aatccactat atctatgtgg ataaaagtta tgacatccta tcaaattttc tttatttggt
     6601 tggtagtcca ttattgtatc tatataatta gtattattac tcatattgat ttctatttta
     6661 agattttgag gattaaaagt cattaatgcc tcttcagaac ctgtaaatcc catatcaagc
     6721 cttattccgt ctgccttcgt ctttttaaaa aaattcaaat ctttgtagct tatttctaat
     6781 tcaccaaata ctctgggatt tacatcaagt attacctcaa aacctttaaa cttagcgtat
     6841 tcatttatac ttttaaattt tttaattata ttttctttag tatctttaac tgataataaa
     6901 catgaaaata tacgtgtaaa tccattttca gcagctttat ctatataatc atatatttcc
     6961 ttagatgttg ttttttctgg ataaattgat attcctaatc ttcccatctg tgtcatctcc
     7021 ttatatccta tatgtgttta aatttatttc gaattacacg aaactttttt aaaaatttga
     7081 tgaaaaaaag tttcatatat atttataaaa aaagaaaagt ttaccttttc tttttttata
     7141 aacaatcaac aaaatatctt cgttacttat ttcataactg ttatactttg aaatttatac
     7201 taatctaata tagtttttaa tttccaatat agtttctaat taataagctt cttgctcttc
     7261 aactttttct tcatgaagca acctcttatc gtacatcttg acaaatggat agtaaattat
     7321 tattgatata gctatacata ataagttaag aactgaagct ctccaatctc caccagtggc
     7381 taaatatgcc cctactggtc ctggtaatgt ccaaggagca gtaactacta ctctggatac
     7441 taaattagca ctcatagcaa accatgaaac tgttgccatt actattggtg ctacaacaaa
     7501 tggtattatt aatattgggt ttaatactat tggcgctcca aatattattg gttcatttat
     7561 attgaatatt gctggtatta cagttgcttt tcctaaagac tttccataag ctgattttgc
     7621 tctaaatgca agaagtaaag ctaagcctat tgtagctcca gaacctccaa tccatataaa
     7681 ccattgaaaa aagggttcag cagcaatacc tggaactgct tgacccgcag catatgaagc
     7741 tgtattagca tctaataatt gtatccataa aggtcttgcc aatgaaccta ttattgatac
     7801 accatgtatt ccaaatgacc aaaaaaacgt tattaaaaat attagtataa ttactgatgg
     7861 taatgtgtca gtggctgaaa ctaatggagt tactaattta gctatgaagc catgccaatc
     7921 aaaccctagg aaataagtaa caatagccat taaaattatt attaatgcgg ctggtgtcag
     7981 agcttcaaaa gaccttgtga ctgatgctgg aactgcttct ggcatggtta tcttgaattt
     8041 agatttgtca gtaaatctat aaatctcaac acctattata gatgttacaa tagcaacaaa
     8101 cattgaacct ccaccaagat tagccattgg catttgccat ccttgaggaa ttgaattata
     8161 ccaagctaaa agttctggac tcttttcaac tatctttaat aaatcagggg taagaccctc
     8221 tattgcagta ggaactattg tcaaaaggaa tgtagctgta gctaataaac ctcctgtcaa
     8281 tccatctaac ttatatgact ttgccaaact ataacctatg ccaaatgtag aatatactga
     8341 tataataaaa aatgtcattc tatatggtag taatattgta actgcatttt cctttagata
     8401 ctgagttacc ccccatgaat ctggtaatgg aggaaatgct aatatcaaga aaaatgaccc
     8461 aactattata agtggtaatg aagctacgat tccatctctt acagctctta agtgacgttg
     8521 ttctgccaat ctggccatag aagttgataa tttagtgtct aaaaattctg taaatttatc
     8581 tagcatcttt tctctcctta attaatattt atttttatta tcattaaaat ttttataaaa
     8641 ttaactttct tctaatctaa caacactgca actactattt actcaacagc tcttgaactt
     8701 gttttaataa ctttggtcct cctaatggac tataagcttg tgcttgtatt aatgcacatg
     8761 gtacactaac ctcatcagca aaagctttta aatagtcaaa tctatgtcta acttgtggag
     8821 ctaccatagc aatatcccat ccatttttaa cttcatcctc aaactcttga gtaccaacag
     8881 ccaaaacatc tatatctatt ccattttttg ctccttcttt ttgtaatgca ttaactgcta
     8941 tagcacttga catccctgat gaacaaacaa ataatactct catattgatt ccccctgttt
     9001 ttttatttct tttcaatcat tatagcatgt atttttgatt acaggaatac gtttgtatat
     9061 atttttttca tattttacat tttaaattgt aattttattt catacattta tatcttgctt
     9121 taattgcttt atttatctaa tcattctttt gtctaaaatt acacactact taaaatactc
     9181 atatcttttc ttaaaaaaga aaaaagacta tcttattatt aacataaaat agtctcaata
     9241 cttttttaaa tatgtattcc tttatatatc cagaagtaac ctattattgc aaatgataaa
     9301 gcaaataaaa tatacagtct aaaaaatccc aaagaaaaac ctctcttagt ttcaacaaag
     9361 tatcttgtat atgcaactgc aaaagatgta attaatgaat ttcctataac aactccaaat
     9421 ctattatcta ttccaaatat agataataac aaaggaaaaa ctacagtact tactagagat
     9481 gaaactatta atattaatcc acttttaaaa cttatagttg gaatattata atctgttctt
     9541 tgatttttct ccatttgttt cttagtattt ttattacttt ttgtactttt tgcattttta
     9601 taattattat ttttctttct tttctttttt ttattattag acatgtcatt tacctccaaa
     9661 aggtttaggt gttatattcc ctaaaataac tttattttta gcccctgttg cagatattac
     9721 attagaaaaa gaattattta atgtttcatt gcccaagatt acaaatgcca aagcctcctt
     9781 tgcatcagaa gaatacccat attcatcttg agttaatact tcaacctcta ataataactc
     9841 ttttatatat cctattaaag ttagattatg agctcctcca cctccaatta ataaagtatc
     9901 tacattaacc tttggaagta tgaagttttt ataattataa tatatagctt ttgccgtaaa
     9961 catagttact gttgctatca aatcatattt atctacatgt ttatatttgt taagcatgtt
    10021 atccaaataa acttgtccaa atacctctct tccagtgctt ttaggtggcg cttgactaag
    10081 atacttatga ctcatcaaat cttttaacat ttcttcatta attttacctt ttgatgcaaa
    10141 gtatccattt ttatcatact ttctattaaa taatctttga catactccat ctattatcat
    10201 atttcctggt cctgtatcaa atgcaaatac atcatctatg ttacatgatt taggtattat
    10261 agtaatattt cctattccac caatattttg aagtgctaaa tttttattgt tatcactata
    10321 taataaaaat tcactatatg gtactaatgg agcaccttct ccacctgctg ctatatccat
    10381 tactctaaaa ttagatatta cctttgtatt tgtttcatat gctataactg ctggttcacc
    10441 tatttgaagc gttgaagata caaaattatt ataacttctt ggtatatgaa agattgtttg
    10501 accatgagat gcgataaaat ccaaatttgc tatatgaaag tttgctttat gacacactga
    10561 ttttacagct ttactaaata aatatccaag cttaaaattt aagctacaga ttaaatcaac
    10621 actcgattcc tcttctatac agcactttct tatttcgtct tttatatctt taggaatttc
    10681 tagagttata aattctattt gcttaacttt agtatttcta ccattcccac taatttcagc
    10741 tagtactgca tcaactccat ctaaggaagt tcctgacatt aaacctacac tatacatagt
    10801 tatcatcctt ttaagtttat agttattcct ccccaaatca aggtattaag tttaataatt
    10861 ttagaattaa tcttatttca tctttaatta cgataattta ctcttatgaa attaattgag
    10921 ttaatcttat acacgttttt taccttagct aaatcttaca ttaatttatt ttcagtgtca
    10981 atgtaatacc atgtaatttt ttttcacaaa caaaacatat tctctctcca aaaaccatat
    11041 taaaatagtt actacaaata aaaaacatac caggtaccta tctgatatgt ttttcatata
    11101 taataattat tattttataa tatagataga gcatcattta aggcactaat taaatcttca
    11161 ctattttcaa gaactaaatt tattcttatt gtatatgtcg atttgtatat gatgatagat
    11221 tttatagtct aataacctaa ctatctaggc ttaaataatc cgatatatca taaaaataga
    11281 tattaaaaat tgtatatgaa ccattttccg aacatacctc aatctcatgc cctaaactat
    11341 ctatcatttt ctttacaaaa tacatcccca ttccagtaga cttatacaat tgatttcttc
    11401 catttttccc cgtaaatccc ttgtcaaaaa ccctagatat atcttcctca tttataccta
    11461 taccaaaatc ttttatagat aataccactc cattttctaa cttttttgaa ttaaattcaa
    11521 tcttaccaac ttcctttgaa tactttatag cattattaat aatttggtct aaaacatatg
    11581 acatgcattt agaatctgtg taaacatcat tttctaattc attcaaacta acttctacat
    11641 tattttttat aagcaaaaat gaattatttt ttatagacat ttttatcaat ttttctaaat
    11701 taaatttatt tataaataca tcttcaaaca tagtgttgct tctactacta tacaaaatag
    11761 agtttactaa aaaatttatc tttgcaacct gattcttaat atccaaacta tcttctatgc
    11821 tacttaacct atctgtaatt atacttaaag aagatattgg aatttttatt tcatgaaccc
    11881 atttagctat gtactcatcc aattctttta atgattgttt ataagtctct atgtcactat
    11941 tattttttat ttttaagttt tctataatat cttttattag attttcttca aaagattgtc
    12001 catcaactaa ataactgtct atttcaccac tattttctat acaattatat aaatttgtgt
    12061 aactatttct aaaatttata taatctataa caaaaaatac cattattata aacagtacta
    12121 aaaaatctaa atatattaac tctgaatatt tatttttaaa aatatctata gcaaataagt
    12181 agatatttat tatactaact acaactaaag gtagagcaat atacttagac ctacctttca
    12241 aatattttac tagcattgta ttatatatcc tacccccttc ttagttttta taaaatcatc
    12301 aaaacctagt tctttaactt tacttctcaa tctattcata tttacagtaa gtgcattgtc
    12361 tgtcacaaac tcatcattat cccatagagt catcataaga ctttcccttg atacaaccct
    12421 attcttattt tttaataata atgttaaaat ttttatttcg tttttagtca actctatact
    12481 tttatcctta tacttatgtt ttataatccc tttccccaca tcaaacatca aatcttcaca
    12541 atacaaaata ctattagaat cactataatt atagactctt cttattatac catttatttt
    12601 ggtaaccaat atttctatag aaaatggttt ggtaatgtaa tcatctgctc cattatttat
    12661 acccattatt aaatccatat cagaatctct tgatgataaa aatattaatg gtacatttga
    12721 aatagctctt attttttcgc accagtaaaa tccattatat aatggtaaat ttacatctaa
    12781 aagtactaca tctggttttt tatctataaa ttcatctaat actacctcaa aatcatcaat
    12841 taattctact ttaaatcccc atttttcaag tgctaatgca atctcttttg ataaatcaat
    12901 atcgtcctct actaataaaa ttctatacat ttgtatattc tccattatcc taatatttca
    12961 ctatgctatg aattattaat atcataacat attttatatg aatttattac ccaaattatt
    13021 tagttaatca acaaggagac tcttattaca agaatctcct ttctctttat taatatatca
    13081 actaattttt atacactaca ctcttaaact tattaacaga tactgctcca tatattatga
    13141 atattatcaa acatatcact aaacttagta tatatatacc aaataaatcg gtcatcaata
    13201 aatctcctgc taatctcaat gcaaaataac tagatatagt tgcacttaac attggcaaag
    13261 tataaaacat agctacttct ttaagtatag ctctatttat atctttttct gtagtaccta
    13321 tttttaacaa aatcttatat ttctctttat cttcatatgc atcactatat attttcatat
    13381 atattatact tccagctaca attgcaaaga ctaagaataa aaacacaagc actacatatg
    13441 caaacttcat ccattgactc tcaccttgag ctttatatcc attttgaata tacccatttt
    13501 ttaaacttaa attcttttca atattgttga acattacttg actattttct tcttgaaata
    13561 cctttgcacc ataaaaatac acttgttgac ccttatctct tagtttaaaa tatgtatcat
    13621 catttaccac tataatactt tgattaagtc ctgtccctag tactggaact tttacttctc
    13681 cccttgaaat attatataac tgaccatcaa gttttatctt ggcttctttt gtagctattg
    13741 atgctatcgt ttgagggctt tttaactcta ttacattatt tccactaact aatctttcac
    13801 catactcttt taattcttta tcattgccat tcactttaag agtattacaa aaatcactat
    13861 atttcattat agtatctgtt gtatcataag cttcttttat atctgttttc tttaaaattg
    13921 ttagttctgg atttactgag tactttatct tattagtatc ttttattata ttctctattt
    13981 gttttttatt cacttcatct ttatccattg aaactatgct aatagtgtac acatttcttt
    14041 gttcttgggc attcttatgt atacgcttca tcgcaactga agcaccaaga gttgatattg
    14101 ttgtagttat aattatagct attatagcat aagttttata gtttttattt aatcggtagt
    14161 ttatattgtt tattgcaatt atattatttc cattaaataa tattttttta tttttgatta
    14221 taatatcaaa tataacaggc ataactgatt tgaataatcc ataagtacct actattatta
    14281 tacctataac aggtagtaca ctagttatat ctccagttct tatattggta gaaagatagt
    14341 agccatatcc tattactata attgatacta tagctaatat atatatacct attcctacct
    14401 ttggtatttt ttcttgtttt ttagatatat tgattagatt tattacacta cttctacata
    14461 ttgttataaa accttttaca gccattatta taaatataga aataaagatt aaagtagcat
    14521 atagtatagc ttctatacta agtccattag atacctttat attaaatcca gataacttta
    14581 ttatcactac ttgaaaaaat ctagatgtta ttatccctat aattattcct gttatacaag
    14641 aacttattcc tataagcata ttttctacaa aatatatttt tcctatagta tataaatcta
    14701 atcccataaa agacaatatt ccaatttctt tagacctatt cttaaaaaat atatttgttg
    14761 cataccatat aaagaaaaat aaaaatactg atagtatcat tattacagct actaacactg
    14821 atagtgtaaa ttttttattt tcatcttgta acacatttat agcatcacca tatcttaata
    14881 attcaaaatt agttagcaca actactgaaa aaaccataga tataaaaaat gctagataag
    14941 ttcttatgtt actcttaaaa ttattataag caattccaag tatactcact tacatctcac
    15001 ctcccataga ggctagcata tccataattt ttccataaaa ttcttttcta ctatttcctt
    15061 tgtttaattt acactttata tttccatcat ttatcatata tacttcattt gaataacttg
    15121 ctgttagagg gtcatgtgta atcattataa tagtagcgtt attttctctg tttatttttt
    15181 ctaatgattc taaaagttca tatgctgatt tagagtctaa agcaccagtt ggctcatcag
    15241 caaatatgac tgttggattt gtaattaatg acctagctgc tgcgcctctt tgtttttgtc
    15301 ctcctgataa ctgatatgga tatttatcta aatgattttc taatccaaat tttttagcta
    15361 tagaaaatac cttagcttct atctccttac ttttcttttt tcctaatgct agaggaagtg
    15421 ctatattgtc cattagtttc atattgtcta atagattata gtcttgaaat ataaacccta
    15481 ttttttctct tcttaatttt gatagttcat tattttttac ttttgatata ttaattccat
    15541 ctagtattat ttctcctgaa gtttgtttgt caagtgtaga cattaaattt agcaatgttg
    15601 tctttcctgc accagatgga cccattattg atataaaatc accttcattg actgtaagag
    15661 atatattttt taacacattt gttttaaatc cttttattcc ataatctttt gatatatttt
    15721 ttatttttaa tatttctttc ataataatag cctccagttg ctaatttttt aatatatatt
    15781 tattttaaag gttttatata aataaatcct taagtaaata ttacatttta actgttagca
    15841 ttacactttt gtaacatatg gaagactatt ataattatag tagaaatatc ttaaaataaa
    15901 agcatgattt acttctttgt ataaacaaag aaaagaagct cctaagaagc ctctaatcta
    15961 tacaattatt cttctctata aaaaaccaaa atacacattg attgtggcaa atacagatac
    16021 tataaacaag tgatattact actatatatt aaaacaatta ttcctgaaga taattcaata
    16081 agactatcaa gcaataagct aagcatccaa aaagacttaa taccattaat gtataattat
    16141 acccaatatt aatgctcaaa aaaccagcaa cagttggaat aatcagagat gaacctccca
    16201 taaatatact taaagctatt aattggctac tacttctaag ttttttagga gaaacttcaa
    16261 atattaatct ttttgaagta attaaaatta aaggagtaga aaacacttga aaaacacaca
    16321 gtataataat gatatttgaa tcatttgtta aagcaaataa tagaaactga actgttatca
    16381 taatagcact taattttaat aagtttaaac tgctatattt ttttaataaa taagaaccag
    16441 ccagatatgt tggtatctct aacaatgact gtaaagacca ctttaaagat atctgagaat
    16501 ttgtagcatt taaagctatc attttatcta ttacagtaca gttatttgct accaccattg
    16561 agtataataa gaataaaata catactagta agatatattt tttatctttt aataattcaa
    16621 taatatcttt attagttaca tttattttct tattaatctt ttcaatatca ggtaataata
    16681 gtatatttat aataagtaat actacaatta aaccaatcgc tacagataat ccaaaatatg
    16741 agaatctaag aatcaagtaa gatgctacca tacttccaat agcccatcca atcgacccaa
    16801 aagtcttaat aaatgacaaa taattattaa tattatctcc acaacctatt atccatgtat
    16861 catataaact acagcaagta tttaataatc ctcctgataa agcaacaagt aataagtata
    16921 ttataaaagt tgcagattca gtagaaaaca taataactga tgcaattcca taaactgata
    16981 aactaattaa aacaattttt ttcattgttt gatacttatc agctaatact ccaaataata
    17041 attgaaacaa tatattggtt attgcatagc tcgataatat aattcctctt tcaaacgctg
    17101 agtaacctaa ttttgataag aaaggtgtga attttgttga agcaaaggca aaaataatat
    17161 atgagaaaaa atagaatatg ataaatttaa atttaatcgc tttattaatc ataatcaatc
    17221 aaaattgata actttaatac cactatttcc ttcctgattt aagtttgaaa gatgatattt
    17281 taccgtatta ggattctcta caataactaa aggaatatat aaagtatttt caagtaactt
    17341 attatcaatt tctactaact tttcccccat attaacttct tgaccttgtt taacttctaa
    17401 tttaaagttt tctttttttg ttgttgcact atccaatcca atatgaataa gaatttgaac
    17461 atcattttgc aatttaattc ctatagcatg gttagtttga gcaataaaac ttattactcc
    17521 tttagtcggt gatttaagaa tattgtcctt tgctatcata gcaaatccat ctcccattac
    17581 cttagttgaa aatactttat caggaatttc tccaagatta attatttttc catcaatgta
    17641 tgaattaatt gtaattgctt ctttttttct tttaaagaac attatagaca ctcctctaca
    17701 taattaactt tattctaatt atcattacaa ataacgatat taagcatact gcaattgagg
    17761 aaaaaagagc cttatagctc tttccccatt gtagttcttt aatactgctt atgatttgtt
    17821 taattccaac aaatttataa atataggtaa acatgataac aatagttatt gtgtaaattt
    17881 ggaatatctg ccctaataag actaaataga aagcaatcat gttttctttt atctctttat
    17941 tcttttttat attgtaaata acatatgcac tgtatactgc actaaagtta gagattaaat
    18001 ttaaacttgc attaacatca ttgtttaaaa gttgtgaaaa tgaaatattt gtaaataatg
    18061 atgtcaattc taaatacact atatataaaa caggtaatat tagaaaacaa accataacca
    18121 ctgaaagaaa tatattttca tcttttaata ctgctgtgat attttttatt ttatttgtta
    18181 tcatgttttt gttctcctta tttaaaagtt ggccagaaat ccttattagc cacaatcatt
    18241 tcatctaaga tttcttttgc tacttttgca cttggaacag ttttactcat agtaaacgac
    18301 tgccataatt tttgataact tttttctatc catgcttcta cagctaattt ttcagaagca
    18361 acttgttgtt ccattaaacc tttttggaaa gttggaattt tacctataga cataggttca
    18421 accatatctt ttccaacaag gcaaggaatt tcaaccattg catcatctgc aaaattttca
    18481 atagctccat tattaggaac aatcagtaaa aatctttcct gagtattaaa tgctaatgca
    18541 caagctaatt caacaataaa ttgagaatgt tcacttgcat caaaccatgt atctcttgct
    18601 gtgccatttt ttataattct ttcacattct tcgaatgtat ccttttctct tctatccata
    18661 atttcatttg ctcttgtata atttggatta gcatgttcaa cagtttcatc agccattaaa
    18721 taatatttta aataagtatt aggtaatgta gtcggttcta tttccaataa atcctttgct
    18781 gctctcatag tttctaacca actagattct ttgtgttgac cttgaggtgt aaattcctca
    18841 tatccttttt tagcacaata ttcctgtaat ttaggcatta aatcatttcc ttctttgtcc
    18901 ttaatacttg tccaccaccc gaaatgattc aatccataat atctaacatc aaagtcttta
    18961 cgagaattta aacctaaaac tctagcaaat atttcctcta aacatactgg catatcacaa
    19021 atattaataa ttcttgaatt tggtcttaat acacgacatg cttctgcaac tatagctgct
    19081 ggatttgaat aattaagcat ccaagcattt ggagaatatt tttccatata atcaatcatt
    19141 tcgattattg gacctattgt tcttaatcca taggcaattc cacctgcccc acaagtttct
    19201 tgaccaacaa ccccatattt tagtggaatt ttttcatctt gttcacgtaa agcatataaa
    19261 ccaacacgaa tttgtgccat tacaaaatca atatcagtaa atgcttcttc tggatttgtt
    19321 gtataactaa atttaatttc tggatttttc tctctaacaa ttattgcaca tgcttcagca
    19381 acttttcttt gtctctcatc attattatca tataatttaa ttgattcaat aggaaattta
    19441 gcaacacttt caattaacat tgttacaatt ccagcagtat aagtactacc cccaccagca
    19501 attagtatcc tctgtctttt tctttccatc tttccttctc ctttataata aattttcaaa
    19561 ttcttctctt acacttggta ctgaaagccc aataataatc tgaatagctt ttccattatg
    19621 cactaaacca tgtgcaccta ctgccttaaa atcttcttct ttagaaataa ttgagtcatc
    19681 tttcaaggtt aatctcaacc tcgtagcaca gtttgtaaca tctacaacat tttctgctcc
    19741 accaactaat tttatgtatt cagatgcttg ttgagaggct ttactcaatt tatttccttt
    19801 ttcatcttta gtttctaagt attcattttt agtatatagt tttgtttctt cactatcttc
    19861 ctctctacca ggagttttca tatcaaactt cttaattaag aaagtaaata caaagaacca
    19921 gataaatgaa aatataatcc ctacaacaac ttgaacaaca tataccttcc agtgatacat
    19981 tccaagagga agccaattta atacaaccca gttaataatt cctccaccaa aatcaccaac
    20041 aactcctagg gcaaacgaaa tcgtttgcaa gcatgcatct aaaaatgcat gtactaggaa
    20101 taaaagtggt gctacaaata aaaatgtaaa ttctaacggc tcagtaattc cagttaatac
    20161 tgcagtaaga gtagcaggaa tcattaaacc taatactttt ttcttttttt ctggctttgc
    20221 agttacatag aaagcaccaa atacacctaa tgaaccaaac accttcgata atccggataa
    20281 tgaaaatccg attggataca tttcctttag agtcttagct gaagtttgaa aatcctgaat
    20341 atgagtagcc caatatactg atgtaccacc tggaacaaca acactatcat aacaaattgg
    20401 tgcatataca aaatgatgta atccagttgg tattaaaatc ttttgtaaga atgcataaca
    20461 ccaaactcct atatttccac tatttataaa aaacgtttgc aaatgtctaa taacattttg
    20521 aatatgaggc caaataaaac aagttaataa tgcaactggt atcataacaa agaaacaaat
    20581 tgcaactaca agtgatgagc ctctaaataa tccaataaaa tctggcagtt ctttatcata
    20641 aaatctatta tgaatataaa tgacaatacc tgatattaac aaagcaccaa ccataccagt
    20701 atctaatgtt ttaataccag caatgcttac aagtccactt ccagcactta tttcagctgc
    20761 gaaatcaact ccaaagcttt ttccccaaaa acttaacata gaacctacaa aataattaaa
    20821 agtcaaatac gttgctaaag cctccataca agctcttgct tgttgctttt ttgcaagtgc
    20881 tattggtaaa gaaatagcaa ataataatgg caattgatta aataccatcc aagctccact
    20941 agatattaca ccccaaaatt ggtaccaaat attttctggt gtagcaagtg aacccataat
    21001 tgactgattt tgaaatacaa ttgagaatcc aaccattatc ccagcaaaag caaataatag
    21061 tgttggtgta aacattgccc ctccaaaacg ctgtattttc ttcatcatat aaattactcc
    21121 cctcctcata tttttagttt atctttggcc ttagatatct attattttaa caggttaata
    21181 aatatataca atctttaggg tacattcgga gcttattcac agtcaaataa gtttttgcac
    21241 atttttatgt tcaaattctc ataaaaaaaa agaatgacta caatttgcaa tcaatccctt
    21301 ctgtaatca
//
LOCUS       DAYAOX010000049        20836 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.049, whole genome shotgun sequence.
ACCESSION   DAYAOX010000049 DAYAOX010000000
VERSION     DAYAOX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.049"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            455..1348
                     /locus_tag="ACGI57_001437"
     CDS             455..1348
                     /locus_tag="ACGI57_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HFG9716518.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1348..2742
                     /gene="gltA"
                     /locus_tag="ACGI57_001438"
     CDS             1348..2742
                     /gene="gltA"
                     /locus_tag="ACGI57_001438"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HFG9716519.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3108..5327
                     /locus_tag="ACGI57_001439"
     CDS             3108..5327
                     /locus_tag="ACGI57_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9716520.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5474..6220
                     /locus_tag="ACGI57_001440"
     CDS             5474..6220
                     /locus_tag="ACGI57_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716521.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6220..8100
                     /locus_tag="ACGI57_001441"
     CDS             6220..8100
                     /locus_tag="ACGI57_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9716522.1"
                     /translation="MDFRKFAYNNIVKNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGVII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAQITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8219..9610
                     /locus_tag="ACGI57_001442"
     CDS             8219..9610
                     /locus_tag="ACGI57_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9716523.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9717..10757
                     /locus_tag="ACGI57_001443"
     CDS             9717..10757
                     /locus_tag="ACGI57_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFG9716524.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11246..11899
                     /locus_tag="ACGI57_001444"
     CDS             11246..11899
                     /locus_tag="ACGI57_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFG9716525.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKAAEKIDGIIDGMKF"
     gene            11975..12184
                     /locus_tag="ACGI57_001445"
     CDS             11975..12184
                     /locus_tag="ACGI57_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716526.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12324..12890)
                     /locus_tag="ACGI57_001446"
     CDS             complement(12324..12890)
                     /locus_tag="ACGI57_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFG9716527.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13051..13704
                     /locus_tag="ACGI57_001447"
     CDS             13051..13704
                     /locus_tag="ACGI57_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFG9716528.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGFSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13862..14038)
                     /locus_tag="ACGI57_001448"
     CDS             complement(13862..14038)
                     /locus_tag="ACGI57_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716529.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
     gene            14566..15510
                     /locus_tag="ACGI57_001449"
     CDS             14566..15510
                     /locus_tag="ACGI57_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFG9716530.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            15532..16158
                     /gene="hisG"
                     /locus_tag="ACGI57_001450"
     CDS             15532..16158
                     /gene="hisG"
                     /locus_tag="ACGI57_001450"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFG9716531.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            16192..17241
                     /gene="hisC"
                     /locus_tag="ACGI57_001451"
     CDS             16192..17241
                     /gene="hisC"
                     /locus_tag="ACGI57_001451"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFG9716532.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            17244..17822
                     /gene="hisB"
                     /locus_tag="ACGI57_001452"
     CDS             17244..17822
                     /gene="hisB"
                     /locus_tag="ACGI57_001452"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFG9716533.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            17824..18429
                     /gene="hisH"
                     /locus_tag="ACGI57_001453"
     CDS             17824..18429
                     /gene="hisH"
                     /locus_tag="ACGI57_001453"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFG9716534.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            18426..19148
                     /gene="hisA"
                     /locus_tag="ACGI57_001454"
     CDS             18426..19148
                     /gene="hisA"
                     /locus_tag="ACGI57_001454"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFG9716535.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            19130..19888
                     /gene="hisF"
                     /locus_tag="ACGI57_001455"
     CDS             19130..19888
                     /gene="hisF"
                     /locus_tag="ACGI57_001455"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFG9716536.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            19916..20584
                     /gene="hisIE"
                     /locus_tag="ACGI57_001456"
     CDS             19916..20584
                     /gene="hisIE"
                     /locus_tag="ACGI57_001456"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFG9716537.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDVIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         8284 a         1949 c         3666 g         6937 t
ORIGIN      
        1 ataaaataag agtatatttt atacaatatg aaagctggta ttgcaataga ttaaagcctt
       61 atacacgttt agtttttaaa gaaattagtg ttattatgaa aacctaagtt aatttattaa
      121 ataaataatt gaataaaaaa attttattat gtatttataa aatttttgtt acaaatttgg
      181 cataatatag tatactttaa gtgtgtatta ggttaaatct ataacaaatt agacgtatga
      241 tatagactta gtgtttattg gcatttacaa gaaatttgtt aatatcaact atttgtagtt
      301 tgggcagtta taattattat aggggggatg tccaatacaa aacaatcaac caagaactaa
      361 tattatatta taaagagtta tagttttgtg tgtttatgtt tttaattaaa tttaaagtgg
      421 gaaaaatata tttgttagaa tggagaatag cgagatgagt aataaaatag ttagtaagag
      481 acaattaaca gatagtattt atttaatgga aatagaggca cctagagttg caaaatcttc
      541 tcaaccaggt caatttataa taattaaaaa tgatgaaaaa ggagagagga ttcctcttac
      601 aatagctgac tatgatagag aaaaaggaac agtaactatt gtattccaaa cagttggtgc
      661 ttcaactaaa aagttagcaa tgtttgaaga aaacgatttt gtaatggatt ttgtagggcc
      721 attaggacaa gcaagtgaat ttatacatga agatatagaa gaattaagaa ataaaaagat
      781 attatttgta gctggtggag ttggttctgc tccagtttat ccacaagtta aatggtttaa
      841 agagcatgga ttagatgtag atgtaataat tggtgcaaga actaaagaat taattatatt
      901 agaagatgat atgaaaaaag tagctaaaaa tgtatatgta tcaacagatg atggtacata
      961 tggatttaat ggaagagtta cagacctatt aaaagattta gttgataatc aaggtaaaaa
     1021 atacgaccaa gcaatagtaa ttggaccaat gataatgatg aaatttatgt gccaattaac
     1081 taaagaacta aatataccta ctatagtaag tttaaataca ataatgatag atggaacagg
     1141 tatgtgtggt ggttgtagag ttagtgttgg aaatgaaact aagtttgcat gtgtagatgg
     1201 tcctgaattt gatggtcatt tagttgattt tgatcaagca atgagaagac agtctatgta
     1261 caagactcaa gagggaagag ctatgctaaa acttgaagaa ggcgatagtc atcatcatag
     1321 taattgtgga tgtggaggta acaaataatg gatgctaaaa aagtaaaagt accagttaga
     1381 gaacaagaac ctgctgtgag agcaacaaat tttgatgaag tatgcttagg gtataataaa
     1441 gaagaagcta tggcagaagc taatagatgt ttagcttgta aaaaaccaaa atgtgtgggt
     1501 ggatgtccag taggaatcga cataccaggg tttataacaa aaattaaaga agatgatata
     1561 gaaggtgctg ctaaagtaat agctaagagc agttcattac cagctgtttg tggtagagta
     1621 tgcccacaag aaagtcaatg tgaaggtgta tgtatacttg gaataaaatc agatgctgta
     1681 tcaataggta aattagaaag atttgtagca gattggtcaa aagaaaatga cataaattta
     1741 tctgatacag agcctaagaa aaatcaaaaa gtagcagtaa taggaagtgg tccagcgggt
     1801 ctagcttgtg ctggtgattt agctaaaaaa ggctatgatg taacaatctt tgaagcaatg
     1861 catgagccag gtggagtttt aacttacgga atacctgagt ttagacttcc aaaacaagct
     1921 gttgttcaac ctgaaataga caatataaga aaattaggtg taaaaataga aactaatgta
     1981 attgttggta aaacaattac agtagatgaa cttatagaag atgaaggatt tgaagctata
     2041 ttcataggtt caggagctgg tcttccaatg tttatgaata taccagggga aaatgcaaat
     2101 ggagtatttt ctgcaaatga gtttttaact agagtaaact taatgaaggc ttatagagat
     2161 gattatgata cacctattag ttctggtaaa aaagttgctg tagtaggtgg tggaaatgtt
     2221 gctatggacg ctgctagaac tgcattaaga ttaggttcag aatcatatat agtttataga
     2281 agaagtgaaa aagagcttcc agcaagagct gaagaagttc atcatgcaaa agaagaaggc
     2341 attatattta atacattaac aaatcctaag gaaattttag tagatgaaaa tggatatgtt
     2401 aaaggtatgg tttgtataag aatggaatta ggagagccag atgattctgg aagaagaaga
     2461 cctatagaaa tagaaggttc tgaatttgta cttgatgtag acacagttat aatgtcactt
     2521 ggaacaagcc caaatccact aatatcttct acaacaaaga gtcttgatat aaataagaag
     2581 agatgcttaa taacagatga aaatggtcag acttctaaag agggtgtatt tgcaggtgga
     2641 gatgctgtta ctggagcagc aacagtaata tctgcaatgg gtgctggtaa aacagcagca
     2701 gcatcaatag atgaatattt aaaagcaaaa gttaatgctt aatttgtaaa ttaaagagag
     2761 ggtgtctcaa aatgaacttt ttagttcatg aggcactctt tttgtacaaa atcaaatatg
     2821 ttttaatcat ttcaacaatg aaaatttcat tcctgtagaa agtctaactt ttaggtctta
     2881 gtacaatctc tattttgatg tagccacttt tttagatggt ttaataattt atcagttatt
     2941 attgtaatat ttgatactaa gtaacataac tttgtctaac tatgataaaa tagttataca
     3001 ataaaggtct tgtaaggagt gaataatgtg tggctaataa aatcagattt gtcaaatcta
     3061 acattaaata atagaaaaat aaattgtgta attaatggta ttttaatatg aaaaaaagag
     3121 aaaaagtatt tgatgggcta gtttttaagc atatattaat cttattaata ctattagttt
     3181 tgattgattt aacaagtacc atcatatatg caagtaactc tatggaaatt acttctagaa
     3241 atatgattga ggcatcaaaa agagaacttg aaaactattt agaagtaaac atatctttat
     3301 taaaagctct tagtcaagat gatagatttt ctaatggtga aacatcacta attgaaaagg
     3361 gaaaattatt aagtccatat caaaaggaat ataatctttt tatgattggt ataacagata
     3421 caaaaggaaa tgcttcaagt acatataggg aaaatgttaa ctcaataaaa gataagccta
     3481 gttttgagaa agcaattaaa acaaagcaag tagtagtttc agatattgaa gtcagtaatg
     3541 taacaggtga caaagtattt atcatatatg taccaattgt aaaaaataat gagatgattg
     3601 ggaccatttt tgcttctttt tattttcaag atgtaaataa tttaataagt agaagcaatt
     3661 ttgatgattc tattaaattt ctaatgatag ataaagatta tactattatt tctcatccaa
     3721 acaaaaaata tgtaaatgat aaaagtaaga tgcttgattt agaagggaat attataggaa
     3781 ccactaaaag tgaaatctta aaaaatataa ataaaaaatg tcaaggtgat tttttatctt
     3841 gggataattg gagattatat aatgtaaaat atacgaacat aaagtggact aattggactt
     3901 tagtatcaaa atgtaatata tttaagaact ttaaaaatct aatagttaat tttatgataa
     3961 agttgtattt ttatatagta atatttatga tattgtggaa attaagtaat gctaaattaa
     4021 tagaacaact taaaaaattg gcttattatg attctttaag tggaataaaa aataaagaaa
     4081 agtttagaaa agattctatg tatattttaa agaattatta tcaagataat ttttacttag
     4141 tacaattgga tgtcaataaa tttaaatata taaatgagat gtttggatat gctgaaggga
     4201 ataagatatt aattcatata tcacaggttt taaataataa tacaaataaa tatgaaattt
     4261 gtgctaggat ggataatgac cattttatat tgttgattgc ttgtaacact gaagatgaac
     4321 ttttaaatag attatctaaa ataaataaag aaatttgtaa cttaaatact actaactcat
     4381 ctaagtataa aattgttatg tcaagtggaa tatataagat tactaaaaag gatgatatta
     4441 aaaagattga cttgttaatt gatagagcaa atattgcagc aaaaagtaaa aaggaaaaat
     4501 atgaacattc atattcattt tttaatgaag atacaagaaa tcgattgtac aaggaaaaac
     4561 gattggaaga taatatgaat aaagctttag aaaaaggtga atttatagta tattatcaac
     4621 ctaagtatag tttagatgat gtaaatgaaa ttgaaggagc agaagccttg ataagatgga
     4681 atagtccaga atttgggttt atttcaccta tagattttgt acctttattt gaaaagaatg
     4741 gttttattgt aaatattgat atgttcgtgt ttgaagaagt atgcaaaaca ttaaacaagt
     4801 ggataaataa agggtatact cctgtaccaa tctctgtaaa tatgtctaga gttcatttat
     4861 atagggataa ttttatagaa aatataactg atttgataag taaatacaat atatctcctg
     4921 agtttattga acttgaacta acagaaagtg ttgtgtttga taatttaaat atattgattg
     4981 atataatgaa aaagattaaa gagataggat tccttatatc aatggatgat tttggttcgg
     5041 gatattcatc tttaaatctg ttaaaagacc tttcttttga tattttaaaa ttagatagag
     5101 gatttttaat cgaaacaaca gatacaaaac gtggaaaaat tattatttct aagattgtgg
     5161 aaatggcaaa agcgattgat attaaagtaa tctgtgaggg cgtagaaact tatgaacaag
     5221 tagagttctt gaaagagata ggttgtgata aggttcaagg atacttattt gctaaaccta
     5281 tggtattaga tgaatttgaa aaacacttaa actttaaatt tgattaatca ttaataatta
     5341 ttagactata atatacccta acaacttact tacaaatatg taagttaatt gttagggtat
     5401 ttttatggtt aaagtagtat attcacgata ttatggtatt gtagtaaaaa taaaaaatta
     5461 tggagaatat aaaatgaaca tattgagtat tagaaatata tcaaaaacat attgcggaaa
     5521 tattccattt aaagcattag ataaggttag tctaaatata gaaaagggag aatttgtgtc
     5581 tgttatggga ccatctggaa gtggtaaatc tactctttta aatataatat ctacagttga
     5641 tagacagagt gaaggtgagg ttgtattaga tggatatgat gtaagtaaat tgaaaggaga
     5701 aaaacttgct gaatttagaa ggaaacaact tggttttgta tttcaggatt ttaatttgat
     5761 tgacacttta actgttggcg aaaatattat gttgccactt acactagagg gggcaagtat
     5821 caaagatatg aatatacaaa ctaaaagtat atctaaattt ttaggaatag ataaaatttt
     5881 agatagaaaa acctatgaaa tttcaggagg tcaagctcaa aggtgtgcta ttgctagagc
     5941 tattataaat aaacctgcca tacttcttgc agatgagcca actggaaatc ttgattcaaa
     6001 atctactgat gatgttttga aattatttac taggataaac aaagaacaaa aggtaacaac
     6061 acttatggta actcatgaag catatagtgc aagttattca gatagagtaa tttttataaa
     6121 agatggttgt atatatacag aaataaaaaa atctgaaagc agtaattctt tctattctga
     6181 tatactagct gttttgtctc aaataggagg tgtaagatag tggattttag aaagtttgct
     6241 tataataata tagttaaaaa tttcagggca tatttagcat attttttaag ttctgttttt
     6301 tctataatga tattttttgt ttttgcagtg tctatgttcc atccaattat aacaaaatct
     6361 ggaatgcaga gtggttctac tgcatttatg gctcttatga cttcagaatt aataatattc
     6421 ggttttagtc tactttttat attgtactct ttaggaaatt tcttgaaata tagattgaaa
     6481 gattttggag tattgatgat aattggaatg agtaacagac aactgaaaaa attgatatta
     6541 attgaaaatt tgataatagg atttttagca gtgatgttag gaatattact tggtttatca
     6601 atttcaaaat tgtttttatt gtatttaagc aaactgtttt atatgaattt aactgaattt
     6661 tattttccag taaaagcaat gctattgact attgtatcat ttatgatttt atttttgata
     6721 acaggaccaa tgagcttaaa attattgagt aaaaagagca ttttagagct tttagtaggt
     6781 acaaaaaagc ctaaaaaaga gataaaatct tcaaagctat ttggaataat tggtgtaata
     6841 tgtcttatat taggatatgc tatgatacta tttggcaata agttaaaaat aaatggaggt
     6901 atgtatacaa cagcactatt aattactttg gggatatttt tatttttttc tcaatttact
     6961 attttgatat taaagtattt aaaaaataaa gaaaaatttt ataagaacaa agtaaatatg
     7021 ttacttataa gtaatttgat atataaatta aaggataata cacgattgtt atttttaata
     7081 acaatacttt tatcggctac tttagtagca tttacaacaa cttcaacact tgtaagttct
     7141 caaggagaag cttctaaaaa taattttcca atggtttact catatttttc agagcataat
     7201 aatgaaaaag aatatgaaga actacaacaa attagagaag caatcaaagg atatgattat
     7261 aaggagttaa gttttcctgt acttaaatat aatagggaag tcttactaag tgtaaagaat
     7321 tataacaaat tatcaaagga attgggatta gataaaatta gttcaaagtt agaaaaatat
     7381 gaaggggtta taattcctag tagcaattca caagaacata taaattcttt aaaacaatta
     7441 aaaaattata gtataaaaaa tttaaatata gaagtaaaaa aatcaaaaaa taaaataatt
     7501 tttcctacag gactgtttag taaagttgta gttataagtg atgacttata caatgaagta
     7561 aaaggtgaat tttcaaaaat taattttcat ggatttgatt ataaaaactg gcaaagcagt
     7621 gcacagataa ctgaaaattt aaaagaaaaa cattttaatt tggatagaga atataacagg
     7681 tcatattttt taacattacc agatatgtat ttagtagagt tacaacaaag taaaattctt
     7741 caatttatgg gtgtttttat aggagtgata ttatttatag cagtttttag ttttttatac
     7801 tttagattat atacagacga gatagaagat aatgtaaagt acaaaaaact atcaaaaata
     7861 ggtctatctt ttaaaaatat gaataagatt gttagtatag agataggtac attgtttttt
     7921 ataccgtatt ttatagcaat tataaatagc ataatttctt tactgttttt aaatcaagta
     7981 ttaaataata gttttaagtt agaaaatttg ggtatattac tactgatatc atctatatat
     8041 tcactttatt tttacttatt aaaaaaaatt tatactagaa aagtttggtt ttttgattaa
     8101 agtggtatat aatagatata taaattaata tttaaaaagt agttaatatt aggggagggc
     8161 atttgcacct cccttgtaat atgtggaaaa ttgaatatta gaagaaggag gagacttgat
     8221 ggaaaatcaa caattattag gaacagaacg aattagtaaa ttactactaa aatattctat
     8281 accagctata ataggtatgt tggtaaatag tttatataat gtagtagata gaatttttat
     8341 tggaaatata ccaggggttg gaccacttgc aataacaggt cttggtgtaa caatgccaat
     8401 aatgacaata atacttgcat ttgggatgtt gataggtata ggaactacta ctaccatatc
     8461 aataaaactt gggcaaggta aagtagaaga ggcaagaaaa cttataggaa atgcaatgac
     8521 gttatctgta ataacaggaa ttatcataat gatacttgga atattatttg caaataaaat
     8581 acttacattg tttggagcaa gtgaaaatac tttaatatat gcaaaatcat atatcaatat
     8641 tatattatta ggtacagtag taaatcttct ttccttttct ttaaaccact caataagagc
     8701 tgatggtagt cctaaaattt cagcaggaat aatgatagta ggatgtttaa ctaatatagt
     8761 tttagactgg atattaatat ttggattcaa tttgggaata caaggagctg caattgctac
     8821 agtaacttct caggctctta cagctatttt aactataggg tattatataa gtggaaaatc
     8881 aaacttacga ttcagtaaat ctaatctaaa attagataaa aagctaataa aagcagtgtt
     8941 tgcaataggt atgtcgccat ttgctatgca acttgcagca agtttagttc aagtaatatc
     9001 aaatattgca cttaaaactc atggtggaga tttagctata ggggctatgg ctactatttc
     9061 ttctattgca atggtatttt tgatgcctat ctttggaata aatcaaggag cgcaacctat
     9121 tataggtttc aattatggag ctgaaaaata tgatagggtt aaaaaagcat atttaggttc
     9181 attagtagta gctactataa ttttatgtat gggtatggta gtagttatgc ttttcccaga
     9241 agctattata ggtatattta ataaagaccc tgaacttatg aatatatctg ttaatggatt
     9301 aagaatatac ttacttatgt tgccaattgt aggcttatca gttacaggaa caaactttat
     9361 acaatctata ggaaaagcaa aaatggctat gttattgagc cttttaagac aagtaatttt
     9421 attgatacca gcagtattaa ttcttcctac attcttaggt ttacagggtg tatggacagc
     9481 tcaaccagtg tctgatttta tagctacagt aattacaggt atagttgtat ttagagaatt
     9541 aaaaagatat actcctaaaa ctgaaaaatt aaatgaaaat gaaagactaa acgagataac
     9601 tactgagtaa aaatataaat tcagataatt gagcaagaaa ttgcaaaaaa tacgattata
     9661 tgataaaata aaaccgctgt caaggataag agaaattcta attatggagt gattttatgg
     9721 aatttacaaa gaaaagcaaa ttaaaaaata tgtatgtaaa catgaatgta gctagaatgt
     9781 tgtctaagat aaatgagtat aagggtagac aactgcttta caaaaaacaa ccaaaagaaa
     9841 ttttagaaaa cttagaaaaa aaatctttag tagattgttc tgaatctaca tacattggaa
     9901 atcaaagaga tagtagtaat tttaatctag aaaagttgat aagtaatgaa gttacaccta
     9961 gaagtagaga ggaattgagt attgttgaat acagagatgt ggtaaaaact attaacagtg
    10021 cttatgagag tattcccatc agttcacaaa cgattcttga gcttcatgga tacttatata
    10081 aattttcatc tactagagga ggaagttata agtcagataa tgattttatt gaacacaatt
    10141 taaaacctag cgaatttata agtattgatt caagtgtcaa taaagttgaa aaagctgtag
    10201 aagaaatatg tgaagcttat aatacattga tagaggaaga tgaaattgat atattaatat
    10261 taatttctgc atttgtgtta gatttcatat tgatacatcc attcaaagaa ggtaatataa
    10321 aaatggcgag agtacttatc ctattgttac taaataaaaa tgggtatgaa gttggaagat
    10381 atataagttt aggaaagata tttgatgata gttcatatga atattatagt aatttaaatt
    10441 atttgaaagc ctcaataggt agtgaaaaag cagatatgaa tgcatggatt gagtattttt
    10501 tagagacgat attaactgca tatgagaagc tggatgatag tttaaatata tctgataaga
    10561 aaagacaaac aaaaactagt agaatagaga aaataataaa ttctaccctt ggatatttta
    10621 caaaagaaga tataagagat ttatgtcctg acattccaga acctactata aatagagttt
    10681 ttaataatct aagaaaacaa gataagatag aagttgttgc aagaggaaga agtgcaaaat
    10741 ggaagaaaaa atattaaaaa atgtttagat aactaaaatg gtatagtgtt ttacactata
    10801 ccatttttaa tgtcattagc atgagcaata atttaacaac ttaagcaata tcaattttat
    10861 ttattgatta tttttgaact gaaaaatggc gttatatggt atacctcagt ggtaattaat
    10921 tttggatgag aagatataat attacagaat atattaggag tatatgtaga aaaaatataa
    10981 taaaagtgtg aggcttagta attaacaaat tatgtggctt cacacttttt tataaaataa
    11041 agatacttag cagaagaata ttttagttga tatgtttata agatggtact tgtttgggta
    11101 aaagtataaa tagtaaaaaa gtagaagtaa tgtgtataaa aacgtaagtt acattctgta
    11161 ataatagtta ttttataatt caacaaaaga atagaatggg tatatttgtg aaataataat
    11221 ataaaataat cttaggagtg agcttatgaa gaaaaaatta ttatatataa atgtaaattc
    11281 aaaacctgaa gacttatctt caagtaaaac tgtagcaaga aaatttataa ataaatttat
    11341 ggaaaaaaat aaagattttg aggttgaaga gatagacctt tataaagaac atataccaag
    11401 acttgagtat cagtactttg aaaaaagaaa tagcatagtt agtgaagaaa atgctaaaaa
    11461 tttagacgat aaggatagaa aagaacttac aaagattaga aatctttgtg accaatttgt
    11521 gagtgcaagt gtatatgtaa tagcagcacc tatgtggagt ttatcttttc cagcaccact
    11581 taaagagtat atagattgta taattcaaga tggaaaaact atatcttttg aaggcaatga
    11641 taaaccacaa ggaattttaa atgatattga tagaagtatg gtatatatac aatcttctgg
    11701 tggacacata ccatgggtgt tgaagccagt tatgaataaa gggttaaatt atgtggaaag
    11761 tattatgaaa tttataggaa ttaaaaaatt tgatgaattg ttagtagatg gaactggaac
    11821 tagtgaagaa gaaagacaag ctgctataga aaaagcagct gaaaaaatag atggtataat
    11881 agatgggatg aaattttaat tataatattt tattaaaaat ataaaggaaa aacctctttt
    11941 aatgtagaaa cttatattga aggaggcgag aatcatgcga gaagaaaaaa gtaatgaaaa
    12001 gtatgattgt tattggtgta atcaagagaa taatttctgt gtagaaataa aagataatat
    12061 agtcatgata gatgatggca ctggtacgtt aaaacaagcc gttttcatag ggtataaaca
    12121 aatccaaatt aatttaaact gttcacattg tcaaaacttg aatagaataa aattaaattt
    12181 gtagaatttt aaaatggaaa ttattgaagg aattaaaaat agaaagtgtt aatatttaga
    12241 ctgaaaatta tattttatac aataaaagtt tatttaaata acattgtatt ttaatattta
    12301 ttttcagtct tactttatta aaattatttt ttggttatgt ctggaaaata tctgtctact
    12361 aatttttctg aaatattttt taattggtca gccattgaag tcagacttaa taaggctatc
    12421 aataaatcat ctttattttc tggatttata ctaatatttt caaaattaga ctctataaag
    12481 tccggtatat tctttctttg attttctttt attaacatga atttttcgta acatgaaact
    12541 aaatcttttt cactgaaatt atccttttca tacacaccat gtaagattct tttaatttct
    12601 tgtattgtaa gtgtattttt catagaataa ataattatca tctgtaaaat ttgttcttta
    12661 gtatactttt ttcccttaac tggtttgatt aatccctctt tgctgtaatt atttatcatg
    12721 gttttagtaa gaattttatc actttcaaat cttttattgg cagaaagctt attgtcaatt
    12781 agagaaataa tttgatccat atataagtca atagatggaa tatcgtttga agttatatct
    12841 gcattattta gtgtttctag tattatagta ttaagttcat tattcatcat atgttacctc
    12901 cataatatat ataatacact aaattagaaa gtaaaacaat attaaggctt gtaaaaaaaa
    12961 cataaagtgg tataattaaa tatataatat agtaatggaa actatatttg tgaatctgat
    13021 ttttaaaata cataattgta ggaggacgaa atgtatcaat tttttttaaa gggtagagac
    13081 ccaataagca gtttaactca ttttataggt gcttgtttat ctcttttagc aactataatt
    13141 ttagtttttc aatctgttac attacaggaa acttctctct tgatgatagt atctgtatcg
    13201 gtatttggat tttcactaat tgctttatat agcgcaagtt cgtattatca ttttttaaag
    13261 gggacaccag aacaagaatt gttttttaga aaagtagacc atgcaatgat ttacgtattg
    13321 attgctggct cttatacacc aatatgtctg aattttatgg aaaaaaaaga aggcataata
    13381 tttgttacag caatatggat agttgcattt attggtataa taataaaaat attttggatg
    13441 gatgcaccta gatggttatc taccagtata tatttactta tggggtgggc aattgttttt
    13501 gatataaatg cattcaattc aattcctaag gattgcctta gactattaat tatggaaggt
    13561 gtatcttact ctataggtgc aattatttat ataattaaaa agcctaatat aagtcctgag
    13621 tttggatttc atgaaatatt tcatattttt attatgatag gttctttatt tcacttttta
    13681 gcagttttat tgtatgtttt atagttttaa aagatttgat taattgatac taaaattcta
    13741 caatgaataa aatggaactg ccttatatag attatatctt cgacaataag acagttcctt
    13801 attataaata ttaaattcta cttataataa aattttgata aaaatagtcc attcaaaatg
    13861 cctattccat aattccttta atgtactttt catttacgac atatagtttg aatttatgta
    13921 ctataattat atcctctaaa ttctctgaaa cactaacaat tctgacacat ttgtttctaa
    13981 tttctacagg taaatctaaa tcatttgtta aattcacata gtcatcatat ttatacataa
    14041 aatcatccct ttacattaag atatacttat tatactatat tttaataagt ttttttaaat
    14101 tattacaatt tttcccgaaa tttcattttt tatacataaa atttccaaat taggtaaaga
    14161 ttaaattttt tattgtataa acaaaagtta catataaaat attttattca attttttaaa
    14221 taactattgc caatttttga aaaatatatt attattaata gtaacaaaaa actgtaaatt
    14281 tagaatatta ataaaaatta tttgggagga aattatcatg gtgagattaa atatagtaaa
    14341 attaaataca aaattaaatc atcaccatca tcatagatta aagggattgt aatatgaaat
    14401 taattcatag acacaagccc tgtttttagt gtaattggtc ttgtatctat gatgggaagt
    14461 aattttaaga gaaaattata gccatatagg tataaagcct atgtggcttt tttattgttt
    14521 taaatttgca aatataagag taaatttaat gaagagggtg agaatgtgaa atttttgagg
    14581 attgaagatg aggttattta ttctaaaaaa agatataaat taaaaaggga aatagataaa
    14641 ctttttttgg atgaagaata ttttcaaata gaaccacaat tatttgaaga atatgatgaa
    14701 tttacaacta taaataataa aattccagaa gaatctatgg taaaggttgt aaatggaaaa
    14761 gtaatggtac ttagagctga tataactaca agtataataa aaagccttgt accaagatgg
    14821 gaagatggtt taaagcttaa gcttttttat aattcatcaa tatataaaaa taaaaataca
    14881 gttggaataa aagagataag acaaataggt tgtgagtatc taggagaagc ctctgtagaa
    14941 gcagatagag aagtagtgaa attagctctt aagatacttg aaaaatacaa taataatttt
    15001 attctagagg ttgggagtag caagtatatt catggtttgt tagaagaatt aaatttaaat
    15061 aagaattgtg aaaatcaaat taaaaattta ctatatacga agaacactca tgaactaaaa
    15121 gtatatattg aagaattaaa aattaagcgt gaagtaaaag agttattatc aaatatttta
    15181 agtttacaag ggaatttata taatgttatt gaaaaatcaa acaagttcta ttgtaataat
    15241 aagatgaagc aagctcttga agaattaaag caagtaaata atttaattga ggaatgtaat
    15301 tttttagata aggctagatt tgatttatca atgattacta tgcttgacta ttatgaagga
    15361 attatgttta gaggatacta cccaaactca tataaagaaa ttcttagtgg cggaagatat
    15421 gattctttaa ctaaagagta tggaaaagaa attccagcca ttggatttac actaagtgtt
    15481 gatgagttaa tgaagtatgt atataagtaa agtttagaag gagggattgc tttggattat
    15541 ttgactatag cactagcaaa aggacgaatt gaaggggaat catttaaaaa atttaaaaaa
    15601 atgggtctag gagacagtat agacacagat acgagaaaac taatttttaa agatgaagaa
    15661 aataaaatta tatatataca tgtaaagcca tcagatgtag ttacatatgt tgaaaaagga
    15721 gtcgctgacc tcggtatagc aggtaaagat actattttag aaaatgagac agatgtatat
    15781 gaaatatatg atttaggttt tggaaaatgt aaatttgctg tagcaggatt aaagggagat
    15841 agcatatata gagaagatga atatttaaaa gtggcaacaa agtatcccaa tatagcaaaa
    15901 aagtatttta aagagaaagg tcaaaaaatt gaaataataa aattaaatgg ttctgtagaa
    15961 ctagctccaa tagtagggtt gtcagatgta attgtagata tagttgagac tggcaataca
    16021 cttaaagcaa atggtcttga aatattagaa gacatatgta atataagtgc tagaattata
    16081 tcaaatagag ctagctatag atttaaatat gaacaaattc agaatataat tcgtttattt
    16141 gaagaacttg ataattaaaa tataattgag ctttataagg agggatttaa gttgagagaa
    16201 aaagaaagca taagagagtt aaggggatat gaaccaaatc acgtaaactg taaagtaaaa
    16261 cttgatgcaa atgaaggaag taagaggttg tttaaatacc ttattaaaga aatttcagat
    16321 agtgatatag atttaaattt atatcctgaa gattcttata gtgatttgaa agaatctata
    16381 atagattaca taaatatttc tggagtaaat aagaaaaatc ttctagtagg aaacggttca
    16441 agtgaaataa ttgatttaat aatacatact tttgtagata aagatgaagt aatattgagt
    16501 ttctctccaa gttttagtat gtattctata tatagtcaga taaatgggtc taaatttata
    16561 ggtgttgaaa gtgatgaaaa tttagttata aatatagata atgtgattga aaaagtaaag
    16621 gagaataatc ctaaaatagt aattgtatgt aatcctaata atccaactgg aacaatatta
    16681 aagagggaag agataataaa gcttttagat tcaactaata gtctagtggt tttagatgaa
    16741 gcatatatgg attttggaga agaaagcatg ttaagtgatg tttttaagta tgataatcta
    16801 atagttctta gaactttatc aaaggcattt ggattggctg gaataagaac aggatatatg
    16861 ttatctaata gtagtttaat aaattctgtt gagaaagtaa gacctccata taatttaaat
    16921 tcattatcag actttattgc tacaagagca cttagaaata aagatgtggt aaaggcttat
    16981 ataaaggaag taaaagagga aagagaagtt ttatatgaag aaatgattgg tatgggaatt
    17041 aaagcataca aatcacaagc aaattttata ttattttatt ctgaaatcga aaatttatca
    17101 caaaaactta ttgatagagg tgttttaata agaaaatttg gtggaaagtt agaaaattat
    17161 tacagagtta caattggtga taaagaagaa aattctatgt ttgttggagc tataagagat
    17221 attttgaaaa aggagaaata ggtatgagaa tttggaaggt agaaagaaat actcttgaaa
    17281 cacagatttt agtagaatta aatattgatg gttctggtaa agctgaaata gatactggaa
    17341 taggattttt agaccatatg cttaccttaa tgtcatttca tggcaagttc gatttaaaag
    17401 tgatttgtaa gggtgataca tatgtagatg accaccatag tgtagaagat ataggtatag
    17461 ctataggtga agcatttaaa aatgcactag gtgacaaaaa aggaatacga agatattcta
    17521 atatctatat acctatggat gaatctctat caatggtagc aatagatata agcaatagac
    17581 cttatcttgt atttaatgct aaatttgata cccaaatgat tggtagtatg agtactcaat
    17641 gctttaaaga attttttaga gcttttgtaa atgaatcgag agtaacatta catatcaatc
    17701 tcttatatgg ggaaaatgac catcataaga tagaaagtat ttttaaagca tttgcaagag
    17761 ctttaaaaga gggtagtgaa atagtttcta atgagatagc atcttcaaag ggggttttgt
    17821 agaatgaata taatagtaga ctatggtctt ggaaatatag attctgtgtc taggggtttt
    17881 agaaaagctg gaatagaaac taaaatttct agtgatatag atgaaataaa acaggcagat
    17941 tcacttatac ttccaggtgt tggagctttt agagattcta taagtgcctt agataaacta
    18001 ggattgatac caataataaa ggagcatgtt tctaagggta aatttatgat aggtatatgt
    18061 ctaggaatgc aattacttta tgaaaaaagt tatgagtatg gagaatatga aggattagga
    18121 cttataaaag gaagtattga taaactggat ataagtttaa aagtgccaca tatgggatgg
    18181 aataatctaa aatttaataa ggcaaatgat gatatactta aatatataaa tgaagatgat
    18241 tatgtatact tcgttcactc atattatgca aattcatcaa atgaagaact aattgcattt
    18301 tcagaatatg aaaagaaaat ccctgctatt gttagaaaag gcaatgtata tggtatacaa
    18361 tttcatcctg aaaaaagtgg agaagtaggg ttaaatatat taagagcata tggggagatg
    18421 ataaaatgat aatatttcca gcaatagata taaaagataa taagtgtgta agactcacac
    18481 aaggagaatt tgacaaagta aatgtatatt atgataatcc attagaagta gcatataaat
    18541 ggaaaaatga aggagcagaa tatatacata tagtagattt aaatggtgct agaagtgaat
    18601 ttggtgtaaa tacaaaaata attgaggaca tagcaaacaa tatagatata cctattcaag
    18661 ttggcggtgg agttagggat aaggaaaaag tcaaaagttt aataaatgct ggtgttacta
    18721 gagtaatatt aggtagtata gcgattgaaa atttgaattt agttgaagaa ttggtcaatg
    18781 aatataaaga gaaaattgta gtttctatag atgctaaaga tggaaaagta gcagttaggg
    18841 gatgggaagt tgtaagcaat gtagactcat taacattatg taaacaactt gaaaagatag
    18901 gtgtacaaac tattgtttat acagatatat caaaagatgg gatgttgcaa ggtccaaatt
    18961 ttgacatata tgaaagaata gcaaaggaaa catcactaaa tgttattgct tctggaggcg
    19021 taacttctat agaagacgta aaaagactaa aagctatgaa cttatatggt gcaataatag
    19081 gtaaagcgct ttatgataaa aagatagact ttaaggaggc acaacaatta tgcttactag
    19141 gagaataata ccttgtctag atgtgagaaa tggaagagtt gtaaagggga aaaaatttaa
    19201 agatatagta gatgttgata gtccagaagt acttggaaaa ttttatagtg attgtggtgc
    19261 agatgaactt gtattctatg atataactgc atcaaatgaa gagagaaaga catctttaga
    19321 atttgtgaca aaagttgcag aaaatataaa tataccattt tgtgttggtg gaggagtaaa
    19381 taaattagaa gatttcactg atattttaag aaaaggtgct gataaagttt ctataaattc
    19441 atcagcagtc aaaaatcctg aattaataag ggaagcttca ttaaaatttg gagcacaatg
    19501 tgttgtttta tcgatagatg caaagaaaaa tgaagagggt tcatggagcg tatacgtaaa
    19561 aggtggaaga gaaaaaacta atctggatgc aattgaatgg gctgttaaag gtgtagaact
    19621 tggagcagga gaaatcgttg taaatagtat ggatgaagat ggaatgaaaa atggatatga
    19681 tatagaatta ttatcaaaga taacttcttt agtaaatgta cctgttatag catctggagg
    19741 cgctggaaaa aaggaagatt tttatgaggc ggttaataaa tccaatgtag atggaatttt
    19801 agcagcatct gtttttcatt ttggcgagat aaaaataaac gatttaaaga aatatttaaa
    19861 agacatgggt gtagaagtaa gattataagt taaatactaa aaggagatta gaaatatgga
    19921 taataagtgt aacaatgtat acagtgatga agtagagaaa tttataagaa gtataaagtt
    19981 tgatgataaa gggttagtcc ctgttgttgt tcaagaggta gttagcaaag atgtactgat
    20041 gttagcatat atgaataaag atgtgataaa gaaaacctta aaagataaag tagcttgcta
    20101 ttttagtaga agtagacaag aactttgggt taaaggtgag acatctggta atactcaaaa
    20161 agtagttaag atgtcttatg attgtgatgt agatactata ctactttttg ttgaacaaac
    20221 tggtgtagct tgtcatacag gcaattattc ttgtttttac agagacttgt ttgatgatag
    20281 ttcaaaaatg gagcttgaag tacaaacaaa tatattgaaa gaattatatg atttaatcaa
    20341 tgaaagaaaa aataatccag ttgaaggttc ttatactaat tatctttttg aaaaaggaat
    20401 agataagatt cttaaaaaag taggtgaaga gagtagtgag gtaattatag catcaaaaaa
    20461 tactgataaa agtgaactta tttacgaaat tagcgattta gtatatcata cattggtttt
    20521 aatgatagaa aaaggtgttg aaattgatga aataaaaaaa gaattactta aaagaagaaa
    20581 atagaataag atagattaat gggtaataag aaaatatacc acttttaata aaaagtggta
    20641 tattttttat tttatatttt gatttataga ataatattta aattacttga taaaattaac
    20701 tatatatata atttattgat aaaataattc tagtacatta tttattttag aaaaaagata
    20761 attatctaat tatatttaag gataataata tgaaaataat ttttatatta aaaaatatga
    20821 atgaaaatag taggtt
//
LOCUS       DAYAOX010000050        20803 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.050, whole genome shotgun sequence.
ACCESSION   DAYAOX010000050 DAYAOX010000000
VERSION     DAYAOX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.050"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="ACGI57_001457"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="ACGI57_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFG9716538.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="ACGI57_001458"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="ACGI57_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFG9716539.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="ACGI57_001459"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="ACGI57_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFG9716540.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="ACGI57_001460"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="ACGI57_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFG9716541.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="ACGI57_001461"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="ACGI57_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFG9716542.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="ACGI57_001462"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="ACGI57_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFG9716543.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="ACGI57_001463"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="ACGI57_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFG9716544.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="ACGI57_001464"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="ACGI57_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFG9716545.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="ACGI57_001465"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="ACGI57_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFG9716546.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="ACGI57_001466"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="ACGI57_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFG9716547.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="ACGI57_001467"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="ACGI57_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFG9716548.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="ACGI57_001468"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="ACGI57_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFG9716549.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="ACGI57_001469"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="ACGI57_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFG9716550.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="ACGI57_001470"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="ACGI57_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFG9716551.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="ACGI57_001471"
     CDS             6989..7174
                     /locus_tag="ACGI57_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFG9716552.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="ACGI57_001472"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="ACGI57_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFG9716553.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="ACGI57_001473"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="ACGI57_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFG9716554.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="ACGI57_001474"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="ACGI57_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFG9716555.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="ACGI57_001475"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="ACGI57_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFG9716556.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="ACGI57_001476"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="ACGI57_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFG9716557.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="ACGI57_001477"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="ACGI57_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFG9716558.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="ACGI57_001478"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="ACGI57_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFG9716559.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="ACGI57_001479"
     CDS             11117..11767
                     /locus_tag="ACGI57_001479"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFG9716560.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="ACGI57_001480"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="ACGI57_001480"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFG9716561.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="ACGI57_001481"
     CDS             12536..12805
                     /locus_tag="ACGI57_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HFG9716562.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="ACGI57_001482"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="ACGI57_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFG9716563.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="ACGI57_001483"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="ACGI57_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFG9716564.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="ACGI57_001484"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="ACGI57_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFG9716565.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="ACGI57_001485"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="ACGI57_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFG9716566.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="ACGI57_001486"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="ACGI57_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFG9716567.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="ACGI57_001487"
     CDS             14901..15848
                     /locus_tag="ACGI57_001487"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFG9716568.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="ACGI57_001488"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="ACGI57_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFG9716569.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="ACGI57_001489"
     CDS             16330..17163
                     /locus_tag="ACGI57_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFG9716570.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="ACGI57_001490"
     CDS             17151..18017
                     /locus_tag="ACGI57_001490"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFG9716571.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="ACGI57_001491"
     CDS             18011..18814
                     /locus_tag="ACGI57_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFG9716572.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="ACGI57_001492"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="ACGI57_001492"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFG9716573.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="ACGI57_001493"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="ACGI57_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFG9716574.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="ACGI57_001494"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="ACGI57_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFG9716575.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8309 a         2449 c         4327 g         5718 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc tagatgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tatcccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtattaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tatagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaca tttaaatttt atttttataa
    20761 tattgtaatt taaaagttta tataaggtta tatattttaa gat
//
LOCUS       DAYAOX010000051        20724 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.051, whole genome shotgun sequence.
ACCESSION   DAYAOX010000051 DAYAOX010000000
VERSION     DAYAOX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.051"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..1512)
                     /locus_tag="ACGI57_001495"
     CDS             complement(<1..1512)
                     /locus_tag="ACGI57_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFG9716576.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKASISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLG"
     gene            complement(1874..2620)
                     /locus_tag="ACGI57_001496"
     CDS             complement(1874..2620)
                     /locus_tag="ACGI57_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9716577.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(2832..4142)
                     /locus_tag="ACGI57_001497"
     CDS             complement(2832..4142)
                     /locus_tag="ACGI57_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFG9716578.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(4155..4625)
                     /locus_tag="ACGI57_001498"
     CDS             complement(4155..4625)
                     /locus_tag="ACGI57_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716579.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(4629..5480)
                     /locus_tag="ACGI57_001499"
     CDS             complement(4629..5480)
                     /locus_tag="ACGI57_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HFG9716580.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            6404..7315
                     /locus_tag="ACGI57_001500"
     CDS             6404..7315
                     /locus_tag="ACGI57_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9716581.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            7490..7987
                     /locus_tag="ACGI57_001501"
     CDS             7490..7987
                     /locus_tag="ACGI57_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9716582.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            8731..9093
                     /locus_tag="ACGI57_001502"
     CDS             8731..9093
                     /locus_tag="ACGI57_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9716583.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            9191..9775
                     /locus_tag="ACGI57_001503"
     CDS             9191..9775
                     /locus_tag="ACGI57_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HFG9716584.1"
                     /translation="MDALREIGNIGSGNAITALASMINSNVEVLIPMVKILEYNEATN
                     LLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAFLDEIEAIEISA
                     IKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRFSDVGDKVLFIE
                     NKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            9772..10257
                     /locus_tag="ACGI57_001504"
     CDS             9772..10257
                     /locus_tag="ACGI57_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HFG9716585.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            10300..10818
                     /locus_tag="ACGI57_001505"
     CDS             10300..10818
                     /locus_tag="ACGI57_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFG9716586.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            10954..11913
                     /locus_tag="ACGI57_001506"
     CDS             10954..11913
                     /locus_tag="ACGI57_001506"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9716587.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            11939..13642
                     /locus_tag="ACGI57_001507"
     CDS             11939..13642
                     /locus_tag="ACGI57_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFG9716588.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            13903..16005
                     /locus_tag="ACGI57_001508"
     CDS             13903..16005
                     /locus_tag="ACGI57_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFG9716589.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            16018..16458
                     /locus_tag="ACGI57_001509"
     CDS             16018..16458
                     /locus_tag="ACGI57_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFG9716590.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            16494..17297
                     /locus_tag="ACGI57_001510"
     CDS             16494..17297
                     /locus_tag="ACGI57_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFG9716591.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDRTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            17337..17933
                     /locus_tag="ACGI57_001511"
     CDS             17337..17933
                     /locus_tag="ACGI57_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HFG9716592.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            18039..18902
                     /locus_tag="ACGI57_001512"
     CDS             18039..18902
                     /locus_tag="ACGI57_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HFG9716593.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            18969..19958
                     /locus_tag="ACGI57_001513"
     CDS             18969..19958
                     /locus_tag="ACGI57_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFG9716594.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            20050..20661
                     /locus_tag="ACGI57_001514"
     CDS             20050..20661
                     /locus_tag="ACGI57_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9716595.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         8122 a         2363 c         3545 g         6694 t
ORIGIN      
        1 tcctagtgga atattaaata ccataaacaa tattaatagt atagtccaca ctattaagaa
       61 tgatatagag tatggtatca tattagcaat tacagtaccc ataccagcat ctttatcgta
      121 ttttcttgtg aatgctagta taatagggaa gtacggaaat aatgggctaa gtggatttgt
      181 tatacaatca cctatacgat aagccatctg agtaagtgct ggattgtatc caaccatcat
      241 aaacattggt acaaatatag gtgcaagaat agcccacttt gcagatgcac tacctataaa
      301 taaatttata aaacatgata atataataaa tcctatcaac aataaaggtc catttattcc
      361 tgctgatttt aaaaattctc cgccctttac agctagtatc agacctaaat tactatttgt
      421 gaataattgc aaaaattgtg aagcagcaaa tgctagtatt atatatccac ccatatcact
      481 cattgcacta gccattatag agactacatc cttatccttc tttattttac ctatagtttt
      541 tccgtataca acaccaggta tgaaaaatgc tagtgctatt ataggtacca tacctttcat
      601 aagaggagca ttgtttgata gtaaatctcc tgtttctggg tcagctaaga atggtttttt
      661 accgattaca ctaagagcta ctataatagc cacatatatt aatatactta tactagcttt
      721 tttaagccct ttcttttcta catcactaag acttccatca acatctagtt ctgcatttcc
      781 ttcatattta ccaaatctag gagctacaat tttttctgtt acccatgttg ccaaaagtat
      841 tagaacaaaa gttgatgcta tcataaaaaa taagttcatt gttgcattgg cttgaaatcc
      901 agaatcaatc atttgagctg ctggaactgt aaaacttgca gcaagtatat cattgacact
      961 tatcataatg tttgcggcaa atcctagaca gactgctgca tatccagaaa ataaacctat
     1021 aagtggatgt ctaccaacac caagatatac aagtgcagct aatggtggaa ataatattgt
     1081 acctgcatct gaagctatat tggcaagcat acctatagtc attacaacaa gagttactct
     1141 tgtttcagga atcttgctta ctgatgcttt gattgttgct gtcataagtc ctgttttgtc
     1201 acatacacca gcacctatca tagttactag aactagaccc aaaggagcaa acccttggaa
     1261 attgctgact atgtttccta aaagaatttg aagctgttct acagtcaata aatttacagc
     1321 ggttatagtt tctccagtac caggatgaac tactgatata cccatagaac caactagact
     1381 agatagtata agtaaaagta cacataaccc taaaaatatt gtaactgggt ctggtagttt
     1441 attaccaaca gtttctattc tgtttaatgt cctgtcaaaa aagtttttct tcttaactac
     1501 gttatcattc ataatatcct ccctaatgtt taaaaatata attaaaaaat aagtattgtg
     1561 gttaatctta caatttaaat tctgtttgtc gatttaagac gtgtccacaa tatgtcctaa
     1621 aaaaagtata aaataaactt tttcctgtgt caatgcagtt ttctgaattt tctggttgag
     1681 tggtgttagt taaatatgct ttaagtagat taatgacgaa ttttatctaa tgtatttaaa
     1741 taacatatgc actttaaaat taattttaaa acaaaatttt atgaaaagct agtggaagat
     1801 taagttatta aaataagtag cgtagtaaag tgttagattt tttaattaat caaataaatt
     1861 tactttgtat tttctacaaa aatctgtgaa tatcttttga attatgtaga ggatataaat
     1921 ttattttatt tacatagatt agattttgtt ctaatttact gtattcaaat ggttttccat
     1981 gtccaggata aatcatttta ggttttgtat tgatgattgt tttccaagat tgaataaatt
     2041 ctagtttgct ttctgcaaat atggtaattt tgtgagtact tggaaatcca ttcattgcag
     2101 aatctccaca aaataaaaca ccttcatcaa ttagtagtga aattgaatca tcagtatgtc
     2161 ctggtgtatc tataattttt ccatttagtt ctttttcaat ttctttacga ttcttgtcag
     2221 acacaagaat acaccttttt tcaaattctt gtttaagtgg agaaaaacta tgctcacctt
     2281 ttccaaatag tttcataatt tggcaaaaga gaaaggcagt acggctggta caaccaccaa
     2341 taaaagagtt ttgaccttta tatagaattt ctaaagcttt atcatgcata acaatctttg
     2401 tattaggaga ttcgtctaga atctgattta gaaaaccaac atggtcatca tgagcatgag
     2461 ttagaaatat gtatcgaata tctttgattt ttatgttgaa tttagaaatt cgttttttga
     2521 aatgtttaaa accattttca tatccagtat caatcaagac aaagccatct tgaatagggt
     2581 acaaccatgt atttataatt cgatttcctg cattaaacat tttttcacca ccttgaatta
     2641 gaatagaaat ttatattaat tagaaagaat atagcatagt tctactgata tttcaacgta
     2701 gctctaaaag ttttaaggag gataatatat tttagtttaa tctaaaaact aaaagaaaag
     2761 aagtatactg ttacaaaaac aaagagtggt ataactttta cagtagatac caccctatta
     2821 taaaaataga cttataaagt tttatcgtgt ttttctaaga atgttagata ttcttcttta
     2881 gtcataactg gagtaaaggt atctaatata tctttagcat ccttagcatt atcaaaaaga
     2941 aggtctacaa ctgtaagtgc cattgcttta gcaggaatta tgtaagctaa atcttcatca
     3001 actatttcaa aatctcttgt atgaagagct ccttttacac caccaatcat agggtgaaga
     3061 gtaggcatta tatgagacac atctccaaaa tcaaaagagc ctgtaaaatc tccaccatca
     3121 ataacttttc cttttagacc taaatcttca agattgtttt taaataaatt atcaagactt
     3181 ttgtatcgta atattggaag atatccagga atttcagtta tctcaacatc agctccaact
     3241 gccattccac cagccatcaa agccttattt attctttcat tagcatcaat cataccattt
     3301 atagttctag ctctaacata tgattccata tgaacatcag caggaacaac atttactata
     3361 tctccacctt ttgtgatgat agggtggaat ctaactcttt cagactcttt aaaagtttct
     3421 ctaagtgcat tcacgttgtt tatagcaagc attgctgcat ttaaagcatt tattccatca
     3481 tgtggagcag aaccagcatg agattcttta cctataaatt taaccttttt tccaacaaat
     3541 ccattactct caggaccaac tagagcctta tcttctccta aatcagaagc atgaaacatc
     3601 atagatatgt ctatatcatc aaaataacct ctttttatta attcttgttt tccaccaaaa
     3661 taagttattt ttccttcttt ttttaattgc tctctgtatt ccaactcaat aaactcttca
     3721 gcaggagtag ccataaaaga tactttacca tctaaatgtt ctaaaacgcc actttttact
     3781 aaaccaacag cagcacctaa cattccagct atttgaatat tatgtccaca agtatgagaa
     3841 gcacctattt cattagcatc tttatgttct ttacatgata taccatccaa ttctccaagt
     3901 atagatatat gaggaccttt cttgctagaa tttgcatctg ctttgcatcc tgtaactgct
     3961 atattttttt ctacagataa atttaattct tgctcaaaaa agtttgaaac agcttctgta
     4021 gttttaaatt ctttatatcc atattctgga tttttgtata ttccttgacc tatttttaat
     4081 attttttctc tattttcatc tatagttttt attactagag attttaattg ttctttattc
     4141 ataataaatt cctcctatat tttaccttga atacttaaaa ctatatgagc tatactcgca
     4201 catgcaaaga atatagttgt aaatacaata agtgaaatta taactatttt ccatgacatt
     4261 tcttttaaag cttctatttt gtttccaaca gaaattcctg caaaagctag tagtggagta
     4321 gttatagcca taaagttaat attattagtg ttagttaaga atacttcaga tgttggacta
     4381 tatggcatac taagtaaaag gccgattata gttgcaaatc caaaagctgg aaagattgat
     4441 ttaggaaata aatcttttat aatcatagct gccatagcag ctaatattag catcaccata
     4501 ccaggaagag ctgtaactat agatgtacca acgctaattc tttgtcctat aagaattata
     4561 actccaataa cagacataac caataattga tatgaacttt tctttaaatt taaattagca
     4621 aacatttatt agcactctcc ttctgtagat ttaatttctt tagttttttt atttctcgaa
     4681 aatttaggtt ctaaaatctt atacataaaa ttagtaaatg gtaaggatac aaaaactaag
     4741 aatataattc ctgttatact agacatcata ttgcttgttg cagcataggc aagtattgta
     4801 tctgccattt caggtggtac catggtagat aatgaaccag cagctgctgt catcatactt
     4861 ccactaccaa caccacaggc cattcccaaa gcatatgggt gaagaccact ataaatagat
     4921 attgaaccta gtacactaaa gtatatagta ccaagaactg ttcccattaa atatgtacca
     4981 agtacaccac taccttctgg tgagtttatc ccatattttt ctgatataac tcctaaagaa
     5041 ggttcacgac ttatacttgc agtagctcca acagcttctc ttttcattcc aaacattaat
     5101 gcaagaggta atgcaacgat tggtgcaagt atatgaccaa attcttgagc gataaacgct
     5161 ggtccagctt gaattatttt gtcaatgttt ggtccaactg tagtaccata ttttatacca
     5221 agcattacta gtgcaattcc aacaacttca ccagcaatat ttatttcttt ttctccaacc
     5281 acttttttaa gtgctttaat ttttttacct aataaatctg gtgttattat aagtccaatt
     5341 ataactgcat aaagcatagg gaacagtact atagaggctt ttccaatagc tatagttctt
     5401 tgccctataa attcacatat tactaccaag attaagcaag ttaaaaatac ttgaataaaa
     5461 tgttttttat taatttccat gatttcctcc ccaaaagaat aaatatttta cttatctgaa
     5521 atgagaaaat acgttttctt aagtataata cgttttccta aataaaatga aaaaactatt
     5581 tcaaataagt ttcagaaatg tcaataacat gtccttaatt aaggataaaa caaaaattta
     5641 acattgtcaa cgaaaaatat ctaaaatatt acatattttg aatatattgt aatatagctt
     5701 tattatgaaa aaattgttta aatattgtag gaataatagc taaaaagctt taaaattagt
     5761 aatataaaat tgaaaaataa taatatagag ttgagaaata gtaaattgaa attttaaaat
     5821 aaatgagtgg aagtagctta gtaaagtatg gaaatgaata ttacaataga aaataaataa
     5881 tatgtaaaga ctttaaatta attttaagta ttaagtacaa atactaattt tgatatagaa
     5941 attgaataaa gtaaatgata ataagcaaat gaattaagat gataataagt gaattgatat
     6001 agaaataagt aaattaagta agacgataac aagtgagata ataataaatg aattgacata
     6061 gcaataagta aattaagtaa gacgataaca aatgagatga taataagtaa attgatatag
     6121 aaataagtaa attaagtaag atgataacaa gtgaggtaat aataagtgaa ttgatataga
     6181 aataagtaaa ttaagtaaga cgataacaaa tgagatgata ataagtaaat tgatatagta
     6241 ataaataaat taagatgata atatccaaag tgagaaagat aatagaaagt aaataaaatc
     6301 atgtaaaaat aatttagata agattctaag attttattta tttaaaaatt agtatgatta
     6361 atttagatta aataaattta ataattttag taggtggtaa tgaatgaaaa taaatagatt
     6421 aacagaaata atagtaattc tattaaataa aaaattagta acagctaaag agttggcaga
     6481 tagatttgaa gtttctacaa gaaccatata tagagatata gaaactctgt ctatgtcggg
     6541 agtaccagta tatatgacga aaggcaaagg aggagggata tctttaatag aagaatattc
     6601 tatagacaag gctatattgt caaaaaaaga taaagagagt ttaatagttg cactgaaaac
     6661 attacaggcg actaagtatc cagaaataaa ttcagtagta aataaaattg gctcaatatt
     6721 tggagagcag aatttttcaa actggattga aatagacttt actgaatggg gaagtaattt
     6781 taatgaagat gataaattta caaaaataaa agaagctatt ttaaggcgta atactataaa
     6841 ttttaattat gtaaatagtt tgtcaagcca gacaaacaga actgtcgaac cactgaagct
     6901 tatgtataag agtaagactt ggtacttgta tggattttgt aaactaaaag atgattttag
     6961 gatatttaga attagtagga taaggaattt atctataaag gatgaagtat tttcaagaaa
     7021 gataatagaa gaagtatgtt tgaatgattc taaagtaatt aaggaaaata ctataacgtt
     7081 gaaattgagg tttaaagaaa agatgctttt tagagtattt gatgatttta ataaggattt
     7141 aataactaaa aatgaagatg atacatatga tgtaattaca gaatttccta ttggtgaatg
     7201 gatttatgga tatattttat catttggtga caatgttgaa gtacttgaac ctaaagatgt
     7261 tagggataat gtaataacta gactaaggga gttgtctaaa atttattcat tataatataa
     7321 tgctagatta aagattttat taaatatata atatcttgca tttagtatat aatatctcac
     7381 tgagtttaat tatattaaaa atatgacata aaggtgtcgt atattataaa ttataattga
     7441 attagttcaa aataaaatag attaataaaa acaaagtgag gtataaagta tgaattatga
     7501 aatagtagag ataaatgaga aaatagttat aggaatatcg aaggagacta caaataaaga
     7561 tggacaagcg gtaaatgata taggtgaatt gtggaaaaaa tttatgggca aagggatata
     7621 taatgctata aagggtaaaa agaatgacaa gacaataggt ctatatacag attaccaggg
     7681 tgattttaca agtccgtata gctttgttgc atgttgtgaa gtaaatagta attctgataa
     7741 agagaagaat ttagaagaat taaacactaa taatactgtt ggagaaagta ttattagtaa
     7801 agttatacca gcaggtaagt atgcaaagtt tgttatagta ggaggtcaaa aagaagtagg
     7861 agatttttgg tttgagtttt ggcaaatgga ttttgatagg acttatataa gtgattttga
     7921 agagtatcaa tgtaatactt ttgatacaaa gaaacaagaa attcatattt acataggaat
     7981 aaagtagatt tgttattact atattgaatg aaatattgag tatagtacat ttattataga
     8041 tagttttatt atttataact ataagcagta aatctgtaaa agatttactg cttttttagt
     8101 aattaatagt atcagtatca ctttgaatct tcagacatat tcttcattta atattgttta
     8161 ttgataaatg atacttgtaa atataatgat ttgttattta atttaaaata tgaaaaaaag
     8221 tattataaac acattagaat acttctggat ttatgagtat acaatttgaa aaatataaaa
     8281 aataatgtat aaagctaaat ctaatttaat cgataataca tatagagaaa tataattaag
     8341 taaataaaca actatctatt agatattgag aaagtcaaat cctatatgaa tctacagaaa
     8401 tgattttaat gaatctgaaa atttttttta tacaaacata aaaaaggtat aaagttgttt
     8461 tattataatt taattacttt tgataacttt ttatgtttta tacaattata tttttaataa
     8521 ctcgtcaaaa cgccaaacat atattacaat agatatataa gtttttttgt tgacaaatta
     8581 tgttaagaga attaagtata atatggcata aataattact tttatttatc aatgaaatgt
     8641 atttattgtg tagcgaaaaa tagtcagata gtatagacaa taactgaaga taaaagcgtt
     8701 tattgttgta catatggagg agttagtttt atggaaaata aaattttgat agtagatgat
     8761 gctattttta tgagaatgat aattaaagaa gctctgagta aaagtggcta taataattta
     8821 atagaagcaa atgatggaga agaagcgtgt aatatattta aatatgaaaa gcctgatttg
     8881 atgttgcttg atattacaat gccaaaaaaa gatggtttag aggtgctaag agaaataaaa
     8941 aagttggatt caagtgctaa ggttgtaatg tgctctgcaa taggacaaga gaatatgatt
     9001 gtagaagcca taaaacttgg agcgttagat tttatagtga agcccttcaa aacagaaaca
     9061 ttagttaaag ttgtagataa tgctttaaaa taaaagtgta aatatacact aatacaatga
     9121 aaagcaaata aatgcaataa taaagggagg ggtttatatt ataaattatt tagaacttga
     9181 tgatgttcat atagatgctc ttagagaaat tggtaatata ggctctggga atgcaattac
     9241 tgcacttgca tccatgataa atagcaatgt agaagtatta atacctatgg tcaagatact
     9301 tgaatataat gaagcgacaa atttgcttgg tggtccagaa aacaaagtag tatgtatttt
     9361 actcgatatg aaaggtgata tcaatggaat gtttatgttt ttgttggatg aatcaataac
     9421 tcaacttatg ctaagttctc tttttaataa agaagaagct tttttagatg agattgaggc
     9481 aattgaaatt tcagcaatta aggaaatagg aaatattatg gcaagttcat atgtaaatgc
     9541 tattgcatct atgttaaaca tgactataag tgtttcaatt ccagatatat gtattgatat
     9601 ggttggagct gttctaaatg tacctatgat aagattttca gatgttggtg acaaagtctt
     9661 atttatagaa aataaattta aaatgagtga taattatttt actagtcaca ttttgatgat
     9721 tccagaaatg agctcactta gagaaatact cgtgaggttg ggattagaag tatgaataga
     9781 gagatagtag tgaacattgc agatatgaag atagcctata gaccaaatgt tttagttact
     9841 tatgcacttg gttcttgtgt aggagtatgt ttgatagata aagtagctgg aattggtgga
     9901 atgttacacg ttatgttgcc atatagtaaa gatgctatta acattgaaaa taagtgtaaa
     9961 tttgctgaca caggaataaa tgagctcatt aaatctatgg agaatatagg tgcaaataga
    10021 atatatataa aagcaaaaat tgctggaggt gctcagatgt ttttaggaag tagcaattca
    10081 gtaattgcaa gtataggaca taggaatgtt gaggcagtaa aaaaaactct tttaggatta
    10141 aatattccaa tattagctga ggacacaggt atgaattatg gtagaacaat acgtttttac
    10201 actgagacgg gtgaattact tgtagattca ataaataaag gtaagaagaa attataatat
    10261 atatttcata gaaagtgtat tttaggaggg aggtattaaa tgagatttcc agatgaccca
    10321 tatttagatt atggactaat tgatgaagga attaagtatc taaagtttaa agtagatgag
    10381 caagattttg gggttgaact tgaaaaggta atagaaatta ctggaaatca agaaattata
    10441 tttattccag aactacctac atattctaaa ggcataataa atctaagagg aaaaatcatt
    10501 ccgataatag atatgcgttt gagacttaaa aaagatgaat taatgactac aaattgcatg
    10561 tatatagtag taactgaaat aaaggacttg gtagttggat ttgtggttga tagagttgat
    10621 gatatagtaa gcttagaaaa aagtaaaata tcacctccac caagagttac tatagaatat
    10681 tcggattatt ttgtaagtgg agttggagaa ttagaaggta aaataattac tttactagat
    10741 ttggagaaac ttttgacaga taaagatgaa gttttaatag atgaattaat caatgggttt
    10801 gattgcacta tcatgtaaca tagggatgta gttggagtgt tattataaaa agattgtgat
    10861 gagaacattt aagaatatag tggtataatt attaagtata aaaattttaa atcattaaaa
    10921 tttgaatata ttatgtttaa gagaggtttg tatatgaaaa ataagcaaca gggaacaatt
    10981 ttagtcgttg atgatagtgt tctgatgtgt gacttaatag ataaaacact tacaaaagat
    11041 aattttaagg ttaaaaaagc atttaatgca atagaggcaa aagaatttat tgaagaattt
    11101 ataccagaca ttattttgct tgatataatt ttaccagata actctggttt tgagttttgt
    11161 aaggaagtaa aatctaatgc tcgtacagca gatataccta taatttttat aacttcaaaa
    11221 aatacagata cagacatagt aaaaggattt ggtgtaggag caattgacta tatggtgaaa
    11281 ccttttagta tgacagaatt aaaagctcgt gttatagctc atcttgaagt aaagaaatct
    11341 caagataaat taaaaaagat aaataatgaa ctggaatctt ctcttgaaaa attgaataga
    11401 ctcgttgtaa gagattatct tacaggtctt tacaataggc gacatataat taatcagctt
    11461 atggagcaaa gaagaattaa taaatatagt gaaacatcaa tatcccttat ccttggtgat
    11521 attgatgact ttaaggttat caatgatact tatgggcatg aagcagggga tttcgtgtta
    11581 acagtaatat caagtataat aaaaaagaat tgtagacaaa ttgatactgt atcaagatgg
    11641 ggtggagaag agtttttgat aatgctcaca aatactccaa tagaaggtgc taagatactt
    11701 tcagaaagaa tccgtaaaga gataaaggag tttgatttca actataaaga acataaaata
    11761 aagtgcacta ttacattagg agtagtagaa gtaaactcaa atatatcatt agaagaaaac
    11821 atatccttag cagataaagc aatgtatgag ggtaaaaact taggaaaaga ttgtagcgta
    11881 gtgagtacaa tgaaaggatt aaaaaagata taaacttact ttatgaagga gtacagatat
    11941 gaaaagagaa gtaaagcttt ctaaattagc aattgtattg ctgattatta tagtattggc
    12001 tgttctagca gggatttttt cttctatttc atcttataaa gcatttgaaa aagtagaaaa
    12061 aagtattaat gatatgaata atttgaggac tcaaagtcag tatataaaag atacaactag
    12121 agagataagt gatgtattaa gaagatatgt atttacagga gaaaaaaaga ataaagatat
    12181 ttatgaaaaa gaattatacg gaaaaacaag agaatcagct ttaaacaaat tagaaaaaat
    12241 tggtcttaca aagagcgagt caaataaaat cttagaagca aaagaaatat cagattcttt
    12301 agtaccaaca gaacaaagtg caataaaggc agtcgaaagt aatgataaga gtaaagctca
    12361 aagtataatt tttggtagtg actatataaa ctcccaagag aaaatggatt ctttaataaa
    12421 tgaatttcaa gaggatatag ttacccgttc aaattccaaa attgtagaag taaaaaaaga
    12481 attacaaagt ctaatattaa gaacaatatt tatactttcc cttttaataa tattgacaat
    12541 aatagaatat acagtaataa aattaaaaat agtgattcct attaataaag tagaaaaaca
    12601 cttttcaaga attgcatctg gggacttaag aacaagtata gacgttgaag aaagtaaatc
    12661 tgaaataggg ctattagtga gttctgtcaa aaagatgcaa agtatgcttt taaattacat
    12721 agaccttata gacagtacat taactagtat tgcaaaagga gatttaagag tagagataga
    12781 ccaagaattt ataggtgatt ttaaatcaat taaaatatct ttagaaagta taataaactc
    12841 ttttaatggt gatttttctg aaataaattt atcagcagag caagttgcaa gtgcttcaga
    12901 acaagttgca gcaggtgcac aagctctttc acagggtgct actgaacaag ctagttcagt
    12961 agaagaatta gcttccacga ttaatgagat atcagatgga attaagaagt gtgcagatag
    13021 tgctagtctt gcatgtgagg tatctgacca atctgcatca caagttggtt ttggaagtag
    13081 atgtatggat gatatgatga catcaatggg tgaaataagt gaaaaatcga gtgaaatatc
    13141 aaaaataatc aaaacaatag atgagatagc attccaaact aatattcttg cattaaatgc
    13201 agctgttgaa gcagcaagag caggtcaata tggtagagga tttgcagttg tagcagatga
    13261 agttaggtct ttggctggta aaagtgcaaa agcagctcaa aatacagcta ttctcataga
    13321 agaagctatt aaaacagtag aaaatggtgt cagaattgct aaagagactg caaatgcatt
    13381 ggacttggta gtaaatggtg tagaaaaaac tactaattat attaatggta tttcttcggc
    13441 tgtacatagt caagaagtag caattacaca gattttatct ggaatagagc aaatttccat
    13501 agtagtccaa atgaactcag caacagcaga ggaaagtgca gcagcctcag aagagatgtc
    13561 tgctcaggct caaattttaa aagaattagt agaaaaattt catctaaagg aaagtgaagt
    13621 aagagaatca tcatgggttt aaattatgag gtgaatattg aagtaagaga attatcatgt
    13681 atttaaatta tgagatgtat atatattgaa ataagagaat tatcaaggat ttaaattatg
    13741 agatgtatat atattgaagt aagagaataa ttgtgtattt aaattatgag gtacatatag
    13801 tgagataaga gaatcatcaa ggatttaaat tatgagatgc atatataata gaaaattatt
    13861 tgtaggtcgt aggtgataaa tgtgaattat attgaaccga gtatgagaca aatgttagat
    13921 atgtttatat ttgagacaag tttattactc gaacaattag atgaaattat gattcaaata
    13981 gaaaaagaga aagtatttac tcataacaac attaatgaaa cttttagaat aatgcatacg
    14041 ataaaaggtt cagcatctat gatggggtta gacaatatat ctgctttagc acatagtgta
    14101 gaagacttgt tttatgctat acgtgaaggt aaaatagaag ttttagacaa tgacctatta
    14161 tttgatttag tctttcaatc attagatttt ataagaagag atatagatga gttacaatca
    14221 agaggttgta tatcttctga ttactctaat tttattgaag gaattgaaga gtatgtgagt
    14281 actttggaat tatcaagttc tatgaaatca aaggataatg caaatttaga aagtaaagac
    14341 gaaaaagaag taatcgattc aaattttgaa tacaaagaag actctgatgt agtaaaaata
    14401 atctttgaac aaggttgcat gatggaaaat gtgagagctt tcatgcttgt atttgaactt
    14461 agagattact gtgaatttat agatttttat ccaaaagatg tagaaacaaa tgcagagtct
    14521 acagagtata ttaaaagtta tgggttttat atacaatttg tagctaaaga ggataaggca
    14581 atagtttata aaactataga agataatctc tatgtaaaag aatacataaa tattagtaaa
    14641 aaagaaatag aaatgaaaag tacatcaaac aatgatgcag tagaaaataa attaaaagat
    14701 aataataaag taagtaaaag ttatcaaaat aactatacag aagatgagat acaagaagat
    14761 gttttaaatg aaaaaactga tatggaaaaa gatagtctaa gtgaagaaaa tgaacattta
    14821 ccaataagta agcagagtat tatgagtgta aatttagaaa aattggactt acttcaaaat
    14881 atagttggag aaattgtaat tatggaatca atggttgtaa atagcgtaaa ttctacaggt
    14941 gttgatatag ataatttcaa taaggcaaca agacaattac acaagcttac agatgaattg
    15001 caagatatag taatgtctat gcgtatgatt caaattgctg gtgtatttca aaaaatgcat
    15061 agaattgtac gagatatgaa taaaaaatta gataaaaatg tagagttaat aactatagga
    15121 gcagaaacag aggtggataa gagtgtaatt gataatttag cggaaccttt tatgcacctt
    15181 ataagaaatg ctatggacca tgcaatagag cctattgagg agcgtatagc gaaaggtaaa
    15241 gaagaagtgg gaaagatagt tttatctgct gaaaatctag gcggagaagc tgtgataagt
    15301 atttcagatg atggtagagg cattatgaga gataagattt tggcaaaagc taaggaacaa
    15361 ggtcttctta aacatcaaag taatgagtat actgatgacg aggtcaattc tttgattatg
    15421 actccaggct tttctacaaa agaatctata actgaatttt ctggaagagg agtaggcatg
    15481 gacattgttc gtaaaagtat tgaaaaagtt ggaggttcaa ttgaagtaaa aagtaaacag
    15541 gatgagggga caacctttac tataaaaata ccattaactc tttctatagt agagggtatg
    15601 aagtttaaag ttggagattc actatttact ttaccaatat catccataag aagaacattt
    15661 aaacttacag acttaaatca aataattaca gaaggaaata agacagagtt aattacaata
    15721 tatggagcat gtattcccat tataaggtta cataaaatat ataatattca aacaaaagta
    15781 accaatctga tgaatggtaa tatgataatt attgaaaatg agcataaagc agtatgcatt
    15841 tttgttgatg aaataatagg taatcagcaa gttgttgtga aaccatttcc agtttatttt
    15901 aaccgattta atataaaaaa taagggttta tcgggatgta caatacttgg ggatggaagt
    15961 ataagtttaa ttatagatgc agatagcatt atagaaatat attaggggat ttaaagtatg
    16021 gctaaagatg aaattattaa acaagtatta acattttatg tcaatgatgt tatttatgga
    16081 atagaattag aaaatgtgat tgaaaccata agatttcaat caataacgta tgtaccatgt
    16141 cttccaacat atataagtgg agttattaat ttgagaggaa gaataatacc agttattaat
    16201 atgcatataa agtacaatct tcctaaagct gattataatg agagaacttg tataataatt
    16261 acaaaggttg atgagtatca agttggaatt attgtagata aagttgttga tgttattcat
    16321 atagaaaatc taaatttact agagacgact aattctaaca acactaatat taataaagat
    16381 ataaaagaga ttgcgaaagt tgaagataat tctatattaa ttttagatat tcgaaagttt
    16441 ttgattaata gcatatagtt agttctgata aattagcagg aggaagtttt tgcatgataa
    16501 aactcacaga tgaagaattt atagttcttg taaaccatgt aaaaaaagaa tatggtattg
    16561 atttaagtaa aaaaagagcg ttaattgaag gtcgcttata caatacaatg atagagaaaa
    16621 aacttagttc ttttagtcaa tatatgaatc tattatttaa agataggaca ggaaatgaag
    16681 ctataaacct aataaataga ttgagtacta accacacatt ttttatgaga gaacctcaac
    16741 attttgagtt tatacagaat agtatacttc ctttttggga agaaaataat aaaataagaa
    16801 acataaatat atggagtgca ggatgttcta gtggagaaga agcgtattca atagctatga
    16861 ctttggatga ttattttgga tataataaag agttgtggaa cattaaaatc atagccacag
    16921 atatttcaac taactcactt gaaaaagcta aaagaggtat atacatagaa tcaaatgtta
    16981 acaatgttcc tgagttatgg aagaagaagt attttataga caacaaggat ggtacattta
    17041 aaatttgtga taagattaga aaaagtgtaa tttttaagcc gtttaattta atgaatgatt
    17101 tctcatatca acattttgat ttgatttttt gtagaaatgt tatgatttat tttgatttaa
    17161 aaacaagaga agaacttata aataaatttt ataatgctac caaaaaagat agttttttgt
    17221 ttgtaggaca tgcagaagta atcaacagaa gtagtactaa atacaaatat gtaaaacctg
    17281 ctatatataa acgataaaaa ggtatttttg atatatgatt ttttggaggc ggtaatgtgg
    17341 aacgttttaa taatatggtt attgctatag gcgcatctgt aggtggtaca gaggcaatac
    17401 ttgaaattat aaaagaccta ccaaaatcaa caccagggat tgttgtagtt caacatatgc
    17461 cagctatatt tacttatatg tatgcacaac ggcttgacaa gcagtgtatt atgaatgtaa
    17521 gagaagctaa gaataatgat agagtagaac aaggaaatgt gttaattgca cctgggggtt
    17581 accagatgaa attgtgtacg gataaacaag gatattatgt aacttgtgaa aaaggagaaa
    17641 gaataagtgg tcactgtcca tcagtagacg ttttgtttga ttcagtagca gaagtagcag
    17701 gtaaaaattc aataggaata atacttacag gtatgggtta tgatggagca agtggacttt
    17761 taaaaatgaa agaaaatggt tcatttacaa ttggacagga tgaaaattct tgtatagtat
    17821 atggaatgcc aatggtagct tttgatagag gttctgttat gttacaatta cctcttggtg
    17881 aaatttcaaa ctgtttgata agacatatta atattttaaa acaaaaagat taatttttta
    17941 taaataaaaa ttaatctttt attaaattat atggataaaa aaccctagaa actccaaata
    18001 taataagaga gatttatatt taggaggaaa atgataatat gaaatttttt aaagtcgata
    18061 caaaagctaa agttttaaga tatgtatttg ttttaacatt gactataatg gctgttttgg
    18121 caattaaaca tgactataaa tataataatt taaggaaaga tgcttcaatt aatttaaata
    18181 gtaataagga agcagtgact gttatgagta accagtcatt accaaagggt aatacaaata
    18241 gtaaacaaac tttatcagta agtacaatag gaagtaaaac aatagtgaaa aatgatgagt
    18301 atttagaaag tactataaac atgcctgtaa taacaaattc aaataaaatt gttgaaaggt
    18361 ctacaaatga taagataaaa aatgacatat ttgaatttta taataaatca tataaagaag
    18421 caaagcagta tcttaaggat aatcctgacg agaaaaataa gtttgttgca aatgtagact
    18481 ttgaacttaa aaagaatact gattctgctc ttagtataaa agtgagatat tatacttata
    18541 gtggtggagc acatggattt tatcaagata tagcatataa tgtggatatg agaactggaa
    18601 aatttttaga attaatggac ttgtttaagg ataatactaa atataaagaa gttatagatg
    18661 aagaaattaa aagacaaata gctgaacttg aaaaaaagga tgaagaaaat atcggaatat
    18721 ataattttaa aggtataaaa gagaatcaaa acttttactt gcaagatgag aatctagttg
    18781 tatattttga tttgtatgat ataactccat atgcagcagg aataccagaa ttttcaataa
    18841 ataaagcgtt gataagttct atgctaaaac cagagtatgt agatttattt gatttgaaat
    18901 agaagattaa attaaatgtt atgctataat atttagcata gagacttatt aaaattgggg
    18961 gtataaaaat gctactttta aaaaaagacg acataaaaaa agtatttaca atgagagatg
    19021 caattgaagc tgataaggaa gcttttagaa tttattgtga aggaaagagt gtaaatcctt
    19081 tgagaaccaa tatatctgtt ccaagtcaag atgctagtat gttatttatg ccaggatacg
    19141 tagaagaatt aggctgtggg ggaataaaaa ttgtatctgt atttcctaaa aatgctcaaa
    19201 aaggcaaacc agtaattcca gcaacagtac tattattaga tggagaaact ggagaagtta
    19261 gtgcagtatt ggatggtact tatgtaactc agattcgtac tggagcagcc tctggagcag
    19321 ctatagacgt attggctaaa aaagattcta agattggagc tttgataggt ctaggaggac
    19381 aaggtgagcc acaacttgaa gccatgatag aagctagaaa tttggatgag gtaagagttt
    19441 ttagtagaaa caaggaaagt agagaaaaat ttgcagaaga aatgaactta aaattaagta
    19501 aatataatac taaaatagtt gcagttgaat catctgacga agccatagat aacgcagatg
    19561 tgatagtttt agctactcca tcaaaacaac cagtacttaa tggaaactta gtgaaaaaag
    19621 gagctttaat aagtgcagta ggttcatata tgccagatat gcaagagttg tgcccaaact
    19681 gtttaactag agcgtctaag atattttttg aatcaacaga tgcagtttta tctgagtcag
    19741 gagatatatt aattccatta aaagaaggta agataagtaa agatgatttt agtggtgatt
    19801 taggaaatgt tataaacgga actattcctg gaagagaaga tgatgatgag ataattgtat
    19861 ttaaaactgt tgggataggt gtacaagatg tcgtaacagc aaaaagaata tacgataaag
    19921 caaaagaaaa cggtattgga atagactgga agtggtaaaa tattcaaaat aattctatag
    19981 ttagacaaat ttatgttata atgttgataa ataaattttt tattatttta attgagggag
    20041 gatgtttgta tgaaaatggt aaaaaagtta atattatcta cgattatggc tctagtttta
    20101 gttctaacta tagggtgttc taatgcagaa gaaaaagtaa agtcgaattt tgaagctaat
    20161 ttagctgcat tagaaatgta taagtatgat gaaaaaggaa tttctgatgc attaacagaa
    20221 gctgaatata aacagattca aacttataac agcaaggaca aggtttcaat gattgctcaa
    20281 ttgacacctc tttatggtaa agaaaatgct gaaaagatat acgaaacaat tgttaaatta
    20341 attcaaggtg ttgaaaagac agtaacagtg gaatcttcta gtaaggataa gataactttg
    20401 aaagttgtaa gtaaagcttt agatactagt aaagtaatga gtgaagcgaa cactttagta
    20461 aaatcttatg taactgatga aaagaattac gataaaaaag aaaaagaagt tcaagatgat
    20521 atgacaaaaa tgattataga cctttacaat aagaaaccaa ctaaggaagt tacaactatg
    20581 attacatata ctaaaaaaga tggtaaatgg caaccaccta gtaatataca aaagttatgt
    20641 gaaggaatat atacaattta atttatttta aagacacaaa actatctttt aagcatataa
    20701 taaaatgttt ggaagatagt ttat
//
LOCUS       DAYAOX010000052        20690 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.052, whole genome shotgun sequence.
ACCESSION   DAYAOX010000052 DAYAOX010000000
VERSION     DAYAOX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.052"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(15..857)
                     /gene="licT"
                     /locus_tag="ACGI57_001515"
     CDS             complement(15..857)
                     /gene="licT"
                     /locus_tag="ACGI57_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFG9716596.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1118..3160)
                     /locus_tag="ACGI57_001516"
     CDS             complement(1118..3160)
                     /locus_tag="ACGI57_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFG9716597.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(3312..4283)
                     /locus_tag="ACGI57_001517"
     CDS             complement(3312..4283)
                     /locus_tag="ACGI57_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716598.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(4284..5261)
                     /locus_tag="ACGI57_001518"
     CDS             complement(4284..5261)
                     /locus_tag="ACGI57_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716599.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5480..7039
                     /locus_tag="ACGI57_001519"
     CDS             5480..7039
                     /locus_tag="ACGI57_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9716600.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGESKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            7145..8104
                     /locus_tag="ACGI57_001520"
     CDS             7145..8104
                     /locus_tag="ACGI57_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9716601.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPAYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLAKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            8107..8919
                     /locus_tag="ACGI57_001521"
     CDS             8107..8919
                     /locus_tag="ACGI57_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9716602.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(9135..10037)
                     /locus_tag="ACGI57_001522"
     CDS             complement(9135..10037)
                     /locus_tag="ACGI57_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9716603.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            10392..11570
                     /locus_tag="ACGI57_001523"
     CDS             10392..11570
                     /locus_tag="ACGI57_001523"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFG9716604.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            11579..12235
                     /locus_tag="ACGI57_001524"
     CDS             11579..12235
                     /locus_tag="ACGI57_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HFG9716605.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            12235..12900
                     /locus_tag="ACGI57_001525"
     CDS             12235..12900
                     /locus_tag="ACGI57_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HFG9716606.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            13005..13781
                     /locus_tag="ACGI57_001526"
     CDS             13005..13781
                     /locus_tag="ACGI57_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HFG9716607.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            13843..15177
                     /locus_tag="ACGI57_001527"
     CDS             13843..15177
                     /locus_tag="ACGI57_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFG9716608.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            15673..16605
                     /locus_tag="ACGI57_001528"
     CDS             15673..16605
                     /locus_tag="ACGI57_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFG9716609.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPNNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNNNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVAT
                     KKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSE
                     GHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(16997..20536)
                     /gene="nifJ"
                     /locus_tag="ACGI57_001529"
     CDS             complement(16997..20536)
                     /gene="nifJ"
                     /locus_tag="ACGI57_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFG9716610.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPVNT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMGICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         7147 a         3259 c         2912 g         7372 t
ORIGIN      
        1 taaactactc tttattataa attcagtaca gatataactc ttttgatatg taatgttaaa
       61 taaagtagtt catcttgact tacatagtaa tcataattct tttctacatg agtttttatt
      121 ttatatgcac aatcataatc cttttcatac tttactttaa ttatatctat aatttcatta
      181 tttgtatcat tgattttttc tttttttact aatcgttttg caaagaattt taaatgagtc
      241 agaagcctat cataatttat atcatcttct ttaaattcaa ctctataata atatcttata
      301 atatttagta tgtcttttac aattttagta attaacagtg attcctttga acttccctta
      361 tagtttgaat ttactatatg cattgctata aaaccagctt catctactgg aaatttcaca
      421 tcaaattctt tatttatata atctacagcc catttcccaa tctcatattc ttttttatgt
      481 attcttttta tctcatgtag taaatcattc tttatagtta tgttttcatg atatctttta
      541 attgcaaatg caatatggtc agtaagtgct acataaatat attcataaag tttagtattc
      601 aaattctcct ctgcataagc aattatttca gtactaacct caaaaaattt ctcatcaacc
      661 tggcttgcta gtttactaat tttatctaaa aagctttcat cttgtattac aaaagttctt
      721 tcaattttac ttttatctat ttcttgacct ttcttttttt gaaacccaat tccacatcct
      781 gttaatatta attctttttt tatattttca tctaaaacta atacaacatt attattataa
      841 atttgttgaa taatcataat caatatctct cccaaaatac aatttccctt attcataaaa
      901 aaatacctaa attaagatac aattcacctg tataccttaa cctaggtatt gcctgcttac
      961 cagtaacaat ccagcttgtt aacgcttata tattgtattc aatttatggt tttattctat
     1021 tataaataaa tatatatgtc aagtaaatta ttgaaaacta tttcctattt tttagtatta
     1081 attttaacat caaaaatcaa attttattcc attatattta tgattctatc atcatcattt
     1141 catcatagag aatattttcc aataactctt tagccacttc tcttgatatt tgcttagttt
     1201 caactaattg ttgaacactg tctctttgac tttgtatcgc cattactctg tagtattcta
     1261 ctcttgcctc aaacttatca tcattttgaa tctttctagt aatccaattc aaataacgat
     1321 tatagaatct gattgctatt aaggcctcat gtctgttatc ttcatcagta tttttattta
     1381 taaatgatat agcagtttta gtagaaagtg catatgcatg gtgaaactct ttgattattt
     1441 ctttattctc agatactatt tttgtaattt tttcttttac ttctttcttt acttttctat
     1501 ttataatcca catgcttatt tttaatttta caattttaaa tatagatttt tgaacagatt
     1561 ttcttgattt tgtaatataa aatctataca atttaaaggc tctatcagaa attttaccat
     1621 cttctaatag tttcaaaaca gctaataatc cctgttggtc tgcttctgca atcatcatac
     1681 ttataagttt attatctgga gtatttagta ttcctttttc tgagtctacc aactgttcat
     1741 ttaaatcatg tataactggt tgtacttctt gtgctttttc attactaagt tttaaaatgg
     1801 cttctttcaa aattagttta tatgcctctt tatcagataa taaaggttct gactcttgta
     1861 cgttattttt taataatctt ggcagtaaaa ctgttgccaa tactaaactt ataagaataa
     1921 cttctgatga tataaataaa acagtatctc tcattggaaa tacatcttta tcagccaaat
     1981 aaaaaggtat agataaggca gttgctagtg taaatgtacc atgaacacca caagtagcaa
     2041 ctattagtga gtatttacat ttagaaatac tctcttttac aacttcttca tccttaaaac
     2101 ctaaaaaacc tacaaaaaaa ttatttaaag gattcttttt aggttttata aaagaattat
     2161 gccataaata tacccatata aatcttataa caaataaaat aagtacaatt aaagtagaaa
     2221 taaaagtcaa ttccaatgcc atattttcat ttttactact aagtcctgta tatatacttg
     2281 gtaacaaaaa accaagcagt gtaaagacaa atccattcaa tacatactcc aaaacatacc
     2341 aagtattgtc tgatattatc cttaatttag tagttgtatt ttgtaaatgc tctttttcaa
     2401 ttccatgagc tatccctgcc attacaacag ctaggattcc agatactcct atttcttctg
     2461 ctacaaaata tacaaaaagt ggagtcatta tttgtataac tattaccatt ggaatttctt
     2521 ccaaattcca cttgtgtaaa ctaagtctta tttttataat tatgtatcct aaaatacttc
     2581 ctactattat tcctccaaaa gctgtaatca taaattgaat ccctgcctct tctattgaaa
     2641 aaactcctgt aactgtggca agtactgcaa ccttaaatgc tactactcca gcagcatcat
     2701 ttaataaaga ttcaccttca agtatactca tcatattttt aggaaaattt aatccttgtg
     2761 taattgattt tactgctact aaatcagttg gagatattac tgctgccaaa gcaaatgaaa
     2821 cagataatgg catcccagga ataagaaaat gtataaagat tccacctgca aacacggtta
     2881 ttaaaactaa accaaatgca agaagcaaaa ttggttttct gagctctaat agctcttttc
     2941 tcgatacatt ttgtccttca ctgaatagta atggagctat gatacatatt acaaatattt
     3001 ctggattaaa tgaaaatgag aatttaaatg gcaaaataga caatataatc cctagtacaa
     3061 tttgatatat tgcaagagga atattactcc actttttgct aaatacagtt gaaatcgttg
     3121 tacaaaccaa taatcccaac aatgttacta aaattgacat aactacctct cttttcaata
     3181 ataatattat agattaaact tatatatcaa ctaacttaat attaacagca tgaaatccca
     3241 tcttaatcaa aaaaattttc agtttattct attattctta ttatatacat caaatttaat
     3301 ttttgaaata tctatactag atgacatgca acaaaatgat ttcttgatat ttcttttaaa
     3361 actggaatct cttttttgca tatttcctta caataagggc atcttgtatg aaacttacat
     3421 ccacttggtg gatttgttgg agatggtatg tctcctttta atataattct atttttctta
     3481 attgtagggt cttgtattgg tactgcgctc ataagcgctt ttgtatatgg atgttgagaa
     3541 tgtaagtaaa gttctccttt agttgcaatt tctattatag aacctagata cataacacaa
     3601 actctatgtg caatatgatt tactacactc aaatcatgag atataaataa atatgaaagt
     3661 ttatatttat cctgtaaatc cataagtaaa tttataattt gagattgaat agaaacatct
     3721 agtgcagaaa caggttcatc acaaactata aattctggat ttaatgctaa tgctcttgct
     3781 atacctattc tctgtctttg acctccagaa aactcacctg gttttctatt taaatggtac
     3841 ttagaaagac cacaactttc tacaacattt aatatattat cttttatttc attctttggc
     3901 actaaattat gttctataac tgcctcggat attatttgtt ccacatttaa tcttggattt
     3961 aatgaactat atgggtcttg aaatatcatt tgcattttgg gtcttaactt tctaagttct
     4021 tccttctcta acttatatat atctacacca ttaaattcaa ctttaccttc agttttttca
     4081 attaatctaa gaatggttct tcctgcagtt gattttccac atcccgattc tccaactata
     4141 gccaaagttt ctcctttttt taaatcaaaa gaaattccat ctactgcctt tacacattta
     4201 atttccttat caaataatcc agatttaact tcaaagtact ttttaagacc ttctactttt
     4261 aataaactct cttctttttt caattatatc tcctcctttt tatataacca acatgcaata
     4321 ctatgaccac tattcaattc tattaaagga ggtatctgag ttctacattt atcacatact
     4381 ttttcacatc tatcactaaa ataacatgaa tctggcatcc caattggatt tggtacttgt
     4441 cctggaatcg aatacaatct tttatctttg ttttttccaa gagcaggctt tgattttaga
     4501 agtccaatag tataaggatg ttttggattt ttaaataatt caacaacctc tgccacttct
     4561 ataattttgc cagcatacat cactaatact ttgtcacaca tttctgcaac tactcccaaa
     4621 tcatgtgtaa tcatcattat agatgtgttt aatttttctt ttatattttt cattatatct
     4681 aaaatctgag cttgaatagt aacatctaaa gctgttgttg gttcatcagc tataagtaat
     4741 tttggattac atgataaagc catagctatc attattcgtt gtctcattcc acctgataat
     4801 tcatgtggat atgaattata tatttcttca gctcttggta tttctaccag ttttatcata
     4861 tcgattacct ttttcttagc ttcttccttt gataaatctt gatgtatcat aataccttca
     4921 atgatttgac ttccaatagt aaacgctggg tttaaagatg tcattggttc ttgaaaaatc
     4981 attgatatct cattccctct tattcccata agttcatctt cattcatctt taaaatatct
     5041 ttgcctcgaa agattatttc tccaccttct atttttgatg ttggaagtaa cttcatcaaa
     5101 gacatggctg ttacagactt accacatcct gattctccaa ctattccaat agtttcacct
     5161 tcatttatat taaaacttac ttcatttaca ctctttacaa tccctttatc tgtatgaaaa
     5221 taaactttta aatctttaac ctcaattagt gctttactca ttattttctc ctctcagcaa
     5281 aattacttaa gataacatac accttttgat tgtattttat aaaaatttaa attacaacat
     5341 tttcttacat tttattattt ttttctatta attataataa tataatagtt aatttctata
     5401 tatattgatt taataatgat tactattata caataaaaaa tatgtgttaa atattatttt
     5461 atggaggttt tataagaata tgaaatttaa aaaattggca tcattaatct tagtttcatc
     5521 attaatgctt acatttacag cctgtgcttc caatgataaa gacaagtcta gtggaagcaa
     5581 atctggtgga actgttgtaa tacctatggc aagtgaccca gatataatca atggagcttt
     5641 tgcaaacgtt aaagaagact ctcttgcatc aaatatagtt tatgctcctc tttatactta
     5701 tgaaaaagga aatttagtaa attatttagc agaaaaagta gattttaaag atagtaagga
     5761 attaactata aaattaaaat ccaatttaaa atggcatgat ggaaagccaa taactgctga
     5821 agatgtctta tttacattta atacagtttt agacgaaaaa caaaattcac catcaagaca
     5881 atacttattg gtgggtgaga aaccagttaa agtagaaaaa attgatgatt taacagttaa
     5941 gattacttta ccaactgcaa gtgaatcttt cttatatggt atatctaaaa tttctccaat
     6001 accaaaacat gtgtttgaag gagaatcaaa tatagctaag tcagaaaaaa ataataatcc
     6061 agttggttct ggtgcattca agtttaagga atggaaaaaa ggtgaaagca tagtatttga
     6121 aaagaatgca gattattttg gtggagaatc taaggcagat tctatagcgc taaaaataat
     6181 tcctaatgaa gcatctcaag aagcagcttt aaataatggc gaaatttcac ttatgaagac
     6241 atctgctgaa ggatatgaaa aggcaaaatc aaactcaaat ttacaaactt atacttatag
     6301 tgaagaaaga ttaaactaca ttgtatttaa ccaaaatata tcaaacatgg ctaacaaaga
     6361 agtaagacaa gccttatcat atgccttaaa tagaaatgaa atgatagaat cagcatatgg
     6421 aaaagaaggt tctgtacctg ctaaatctat attagttcca gaagcagact tctatacaga
     6481 agaaggtgtt gaaggatatg accaagatac aaataaggct aaagacttat tggataaaag
     6541 tggtgttaaa attgataaat taaaaatagg ttataacaca ggtagatttg ggcataaaaa
     6601 ctatgcctta gtagctcaac aagagttgaa aaaaattggt atagaagctg aaatagttcc
     6661 ttatgaaagt aaggcattct tcaatatact atttagtaat agcactgaat gtgatatgta
     6721 tgtaaatggt tatgcttggg gattagaacc aaacccatat agaggtatgt ttgaaactgg
     6781 tcaatattgc aaccaaacta aatattctaa tgctgaaata gatgctttat gggaaaaagg
     6841 atttactgag ttaaacaagg aaaaacgtga agaaatatat aagcaaatac aacaggatat
     6901 atctaaggat gcgcctattt acactataga ttatgaacaa aatttaatgg cagctcaaaa
     6961 aaatctaaaa ggaataaaag atgctaaacc atctcctgca atattatttg aagattggtc
     7021 taaactgtat gtagaataat tgatgtaaaa ttttatatta ttaattagaa aaggagatgt
     7081 ctaaaaataa tattgtttta ttattttaaa acgagacatc tcttttaatt aggagaattt
     7141 acttatgtta aaattaatat tgaatagagt aaaagtttta atacctatgt taatcttaat
     7201 atctatatta tcatttggac ttttagaatt agctcctggt gacccagcag atgcatatat
     7261 aaatccttta atgtcttcac aggatattga aaatataagg gttaatatgg ggcttgataa
     7321 accagcatat ataagatatt taaactggtt gaaaaacact ttaagtggaa acttaggtat
     7381 ttcttatata aaccatatgc ccgttacaga acaaattatg gaaaaaatgg gaaatacatt
     7441 tattcttatg ggtacttctt tgatattttc aatattggta gccatacctc ttggaatatt
     7501 tttagctgta aataaaaatt caataactag taaagtatca tctgttttta attatatagg
     7561 tgtttctata ccttcttttt ggataggtat gatactcatt tcaatttttt cagttaaact
     7621 taacattttc ccatcaggag gtatgcatac tattggaaat gatagtattg gagatttagc
     7681 aaaacattta gttttgcctg taataacttt aggtctatat aatactgcta tttttacaaa
     7741 ttatgtagaa gctagtgtaa atgaacaatt aaaaaaacaa tatgttatta cagcaagagc
     7801 aaaaggatta tcagaaaaag tcatactatt taaacatgtc ttgaaaaatt cactgacatc
     7861 attggttaca atcctaggca tgagtataca aaaattagtc actggtgcat ttgttacaga
     7921 agtagttttc tcttggccag gtatgggaag attaatgatt gattcaattt tttcaagaga
     7981 ttatactgta attatggcaa taactatgtt atcagcacta tttttaattt taggtaattt
     8041 agttgctgat atactatact tattaattga ccctaaaata aaatctagca aaggaggatt
     8101 ctagttgtgt ttacaagtat cttaaaaaaa ttaaaaaaag ataaatcctt tgcttttgca
     8161 tgtattactc ttagcataat aatcatatta attatagctt cttttatatt aaatatagac
     8221 tctaatgcaa ttgatactac ttctaaactt caagaaccta gcttaaagca tatctttggt
     8281 acagacgaac ttggtagaga ttacttctct agagctcttt atggtggaag gatttcactt
     8341 agtgttggta ttttatcaat gcttatttct gtagtatttg gtactgctgt aggtgttatt
     8401 agtggatata ttggaggtaa gctagaccaa ttcttgatga gattagtgga tatgcttatg
     8461 agtataccaa gtttcttagt tttgattgta ttaaatactt atctaacacc taaagtttcc
     8521 actattatac taattattgg attcttaagt tggatggaag tttcaagaat tgtacgtggc
     8581 gaaacattaa aaataaagga aaatgaatac tgtctagctg ctaaagctac tggtatcaaa
     8641 actagaaata tactattaag acatatagtt ttaaacttaa aagagactat tgtagttgct
     8701 ggttcgttaa atattgctaa tgctatctta actgaatctg ctcttagttt tttaggtctt
     8761 ggaatccaat tgcctcttgc atcatggggt aatatgcttc aagatgctca aagacatatt
     8821 tttgacaagt tatatttagc tgtatttcca ggattactta tatttttaac agtcatgagt
     8881 ttgactataa taagcaaaaa attttctagt aatacttaac aaattttata tagaaaataa
     8941 aaacagtgat tatctaaaga tatacactgt ttttattttt cgtttaaaaa aatacatatt
     9001 ttgtatatta taactttaaa tactactcta ccatgttatg caaagcgtat tattgctctc
     9061 aaacatatag atgaaaaact aatagtttat tataataaaa attgatttat ttatttgtta
     9121 ttttaataac atttttaaga gttcttttta ctaaagaaat ctataataaa tttaaataca
     9181 tgttggtcta catggctata acgttctttt ttaggtctac ataatataac tttccatgta
     9241 ataggatgat taaaaggctt ttctataatt ttgctatttt caaaaaaatc atgaactggt
     9301 gatggtaaaa ttgttataaa atcagaattt gtggttgata gcaacaaaaa atcccatgaa
     9361 ccagacatta tatatctttt taacttaaca ttttcatcag taaatttttg cattagctta
     9421 tgatgtatca taaaagtagg attaaatatt gcaagtaact gctcattcaa atcgttccaa
     9481 tctatcttat ccttcttagc aagtggatga tttatattta aaaatgctgt aagttcatct
     9541 tcttgaagca catgttcagt tactacactt ttatcaattt tatgtggatg taacaatact
     9601 gcataatcta attcctgtaa gataagcatt ctatttaagt catatgcacc cttctctact
     9661 atatcaaatt ctatatctgg attatttgca actaactgtg caactacatc tgaaaatgct
     9721 attccaagta taagtggagg tattcctatt ctaacttttc ctttaaattg aactgaatct
     9781 tctcttaaat cttccatcat atttttgtat tcatttatga tatgttctgc atttacataa
     9841 aatctttctc ctgctggtgt taacccattt aacctgcctt tatacctttc aaataaatat
     9901 acatcttcac tttcttcaaa attttttata atttgactta atgctggttg agatatgtga
     9961 agtgtcttag atgctattga taaattaaaa ccagatttca ctatttcaac aaaatatttc
    10021 aactgtttta tatccatttt aaaccccctt ccccaaaagt ataatttaga ttatattttt
    10081 tcttctaatg atattttata ggaaaatcaa cataatttca ttaatatttt aacaaaatat
    10141 ttttctaaat ctactataaa tttttatcct ataaattttt cttatacact atataaaaaa
    10201 ttagacttag acaaaacgct tttttcgccg taatctataa ttagaaacag gaaaacctaa
    10261 agcattagga ataaaagtaa ttgaaatact taaaaacaag cttgtattaa aaaagtactt
    10321 tattatagtg ttccctaatt tctttattca gaaaattttt aattatcatt caagaaaggt
    10381 aactattaga tatgaaagat gtagtaatcg tatcagcagt cagaacacct ataggaagtt
    10441 ttggaggtgt atttaaaaat acttcagctg ttcaacttgg aactatcgct gttaaagaag
    10501 cgatatcacg tgtaagactt aatttatctg aaatagatga agtaataatt ggaaatgttc
    10561 ttcaaacagg gcttggacag aatgttgcta gacaaattgc tataaatgca ggtataccaa
    10621 attctgttcc aagctacaca gtaaataaat tatgtggttc agggcttaaa agtgtccagc
    10681 tagctgccca atcaatcaca tctggtgaaa atgatgtggt aattgcaggt ggtactgaaa
    10741 atatgagcca agctccttat atcgttccaa ctgctcgctt tggtagtaaa atgggtgaca
    10801 taaccatggt agatagtatg ctaacagatg gccttattga tgctttcaat cagtatcata
    10861 tgggaataac tgcagaaaat attgcaacta aatttgaatt tactagagaa atgcaagata
    10921 aattggcatt agaaagtcaa aataaggctg agaatgctat aaaaaacaac cgctttaaag
    10981 aagaaatcgt tccagtagat gttttaatac gtcgaggaaa aatagaaact attgataaag
    11041 atgaatatcc taaattaggt atgacatttg aaggtttatc taaacttaaa cctgcattca
    11101 aaaaagatgg tactgtaaca gctggaaatg cctcaggtat taatgatggt gcggctatgt
    11161 taatactaat gagccaacaa aaagcagacg aactaggtat tagaccactt gctaaaataa
    11221 aatcctatgc ttcagctggt gttgaacctg aagtcatggg tactggtcca atccctgcaa
    11281 ctagaaaagc actgaaaaaa gctggactta gcatcaatga tatagactta atagaagcaa
    11341 atgaagcgtt tgctgcccaa gcattagctg ttaaaaatga acttcaaata gattcatcaa
    11401 agttaaatgt taatggtggt gcaatagcat taggacatcc aataggagca agtggagcta
    11461 gaatacttgt taccttaatt tatgaaatgc aaaaacgtaa agttgaaact ggacttgcaa
    11521 cactttgtat tggtggtggt caaggaatat caatggtggt ttcacgatag gaggaaatat
    11581 gaataaaata gttagcattg atgaagctct atctcatgta aaagatggag atacaataat
    11641 ggttggtgga ttcatggcca atggttcacc agaaaattta attgattatt tatgtagtaa
    11701 aaatataaaa gatttgacac taatttgcaa tgatactggc tttattgaca aaggagttgg
    11761 caaaatggtt gtaaataagc aatttaaaaa gataattgct tctcatgtag gattaaataa
    11821 agaaactgga agacaaatga ataacaatga aacagatgtt gagttagttc ctcagggaac
    11881 tcttgctgaa caaatacgtg cagctggtta tggtctagga ggtatactca caccgactgg
    11941 tattggtact cttgttgagg agggaaagca aaaaattgtt gtcgatagca aagaatacct
    12001 tcttgaaaaa ccaatttatg cagatgttgc tcttctattt gcatcaaagg tcgataaagc
    12061 aggtaatcta gtttataaag gctctatgaa caattttaat aatttaatgg catctgctgc
    12121 caaaattaca atagttgaag cagatgaaat cgttgaaatt ggtgatattg acccaaatga
    12181 agttaataca ccaggtattt ttgtgaatta catcgttgaa ggaggtaatt tataatggat
    12241 aaattagaaa tgcaagaata tattgcaaat agagtatcta aagaattaaa agacggagcc
    12301 gttgtaaacc ttggaatagg tctaccaacc aaagtagcaa attatattcc ggataatgta
    12361 aacgttatct tacaatctga aaatggcttt ttaggtttag gttcagccga agatggaaat
    12421 tcaagtgatg aaactattgt aaatgcaggt ggtcaaccag ttactatact tcctggagga
    12481 tgtttctttg atagtgctac ttcttttgga ataatcagag gaggtcatgt tgatataact
    12541 gttcttggcg cacttcaggt tgacaaatat ggcaatatag caaactatat gattccaggt
    12601 aagatggttc caggtatggg tggagctatg gacctagtaa ctggagctaa aaaagttatt
    12661 gtagcaatgg aacatacttc aaaaggttct gcaaaaatat taaataattg tactcttcca
    12721 cttactgcaa caaatgctgt agatttgata gttactgaaa tgggagttat ggaagtaaca
    12781 tcagatggaa ttttacttaa agaaataaat ccagctttca cattagatga tgttatttct
    12841 gctactgaag ctcctttaat attgtcagat tcattaaatg gaaaaatatc tgttgtttaa
    12901 aataattaaa actagaaaat aaaaatctct tagacgtatt acatataaat ttgtattgct
    12961 taaaatatta atataaaaat aaaataaaat tggaggaaat gaaaatggtt aaagataaag
    13021 ttgtattcgt aacaggagct gcaagtggaa taggtaaaca aatagctgaa tcgttcttaa
    13081 aaaatggtgc taaagtaatg ttctctgata taaatcaaga agctttaaat aataccataa
    13141 cagagttaca aaaaaaaggt tatgattgca tgagtgtaaa gtgtgatgta actaaggaag
    13201 aagaaattaa ccacgctata gacaaaactg ttgaaaagta tggcagatta gatatattaa
    13261 taaacaatgc tggacttcaa catgtttcta tgatagaaga tttcccaact gataaatttg
    13321 aatttatgat aaaaataatg ctgacagcac cttttatagc tactaaaaaa gcatttccta
    13381 taatgaaaaa acaaggcttt ggtcgtataa taaatatggc ctcaatcaat ggtgtaatag
    13441 gatttgctgg aaaggctgct tataactcag caaaacatgg acttattgga ctaacaaaag
    13501 ttgctgctct tgaagctgcc gattctggta taacagttaa tgctttatgc cctggatatg
    13561 tagatactcc acttgtcaga ggtcagttca atgacctttc aaaaactcgt aatataccat
    13621 tggaaaatgt cttagaggaa gttttatacc cacttgtacc acaaaaacgc ctattagatg
    13681 ttcaagaaat tgccgactat gctatgttct tagcaagtga taatgctaag ggtattacag
    13741 gacaagcttg cttactagat ggaggatata ctgcacaata gttattatat ttagggcttg
    13801 aagaaagtat tcttcaggtc cttctactaa ggagggatat ttatgagtat tatcgcgcca
    13861 attggtataa ttattggtat catagctatt atatattttt cagttaaaga aattcatata
    13921 actatagctg ctccaatagc aactttaata gttgttattt taaataaaat ggatgttgta
    13981 acgtcaatgc taggtgctga caaaaacaat tatatgggtg cacttggaaa ctatataatg
    14041 agttattttg caatattctt attaggctca atacttgcta aatttatgga agaaagtgga
    14101 gctactgtat caatagctga ttttatttta aataaatttg gctctaatca tccttataga
    14161 gttttagttg ctatatttat agtaagtttt atattaacgt atggtggtat aagcttattt
    14221 gtagttatgt ttgcggtcat tccacttgca agaactctat ttaaaaaact tgatatttct
    14281 tggaacctta ttcagatacc actttggctt ggtattgcaa caattactat gactataatt
    14341 cctggaacac cagccattca aaatgttata cctattcaat atctaaatac gtctcttact
    14401 gcagctacta taccaagtat tgtaggtagt ataggttgtg ctacatttgg acttgtctat
    14461 atgaaatatg ctctaaacaa aagtattaga aaaggtgaaa cttatgcaac ttacacttca
    14521 gaattagaag aagaatcttt agatagagaa ttaccaggat tttttgcaag tatttttcct
    14581 ctattagcac tcgtaattat agctataact ggaagtactt ttggttcaga atttatgaaa
    14641 aaaaatgtaa tttacatagc tttaatagtt gcaattcttt tatctgcttt tttattccat
    14701 aaatttctat catctaagat acagacattg agcataggtg ctagtggttc tataggtcct
    14761 atatttgcta catcttctgc agttgcattt ggagcagtaa taatggctgc acctggtttt
    14821 gatgtattta gcaaacttat aatgtcaata ccaggtagtc cacttataag tcttactgtt
    14881 ttgacttcaa caatgtcagc aataacaggg tcctcttctg gtgcattagg aatagtaatg
    14941 ccaaactttg cagattatta tttaagtact ggtctaaatc cagaattaat tcaccgtgtt
    15001 gctacaatag cttcaaatat actaactata gttccacaaa gtggtgtttt tcttacattc
    15061 ttaagcttat ctaagttaac acataaaact ggatttaaag aaactttcat tgtagttgca
    15121 gttggttcac taatagcaga aattattgta attacattag gattactaat gtattaatat
    15181 aaaacaagtt aaaattcata atatgaaagg tggtgtctca aagtgaactt tttacttaat
    15241 gagacactct ttttatatga aataacatat gcctatcatt taaacaataa aaaaggcctt
    15301 atctctattt tgagacagac cctttttatt tcttaaagat ttaaataatt ataatttgtt
    15361 gctaatacga aagcatctac tgaataaatt taaccaaata aaaaaaaatt tatcataact
    15421 ataaatttac gttatggaat tattataaaa atattatttt ctctaactaa gtaattccat
    15481 actatacagg cttttaatac aaattgtcaa acacactgaa atatacctat ttattccata
    15541 tatatctttt ttacatatct ttacagtaaa aaaagtctta atcttatgtt agtatagatt
    15601 cacgggtaac cccctacact aaaggttaca aatcagtaac atttttagaa tttttagatt
    15661 aagaggtgtt taatgatgag aagaaatata ccaacaaaat taaaaactat attagcttta
    15721 ggagtgttaa ctttagcaac agcttcaagt gctgttactg caagtgcttc atcacttgaa
    15781 aataatgtaa atataagtaa caaaagttta gtatatagta atactaaata caagttttct
    15841 gcaaaaccaa attgttctgt aaaaccagaa cttaagcctg ataaagataa ctcttgtaag
    15901 gataagaatc ataatgacaa gaattgcaac aacactaata aacctgagga caacaataat
    15961 tcagactcta ctaataagcc tgataacaat aaacctgagt ctaataaacc tgaagacaac
    16021 aataattcag gctctactaa taaacctaat aataataaac ctgagtctaa taaacctgaa
    16081 gacaacaata attcaggctc tactgataaa cctgataaca ataaacctga gtctaataaa
    16141 cctgaagaca acaataacaa taattcagac tctactaaca aacctgatga taataacaac
    16201 tcaggctcta caagtgaaag tttttcagct taccaaaaag aagttgttga cttagtaaat
    16261 atagaaagag ctaaagctgg attaaatcct cttactcttg attcttctat atcaaatgtt
    16321 gctacaaaaa aatctcaaga tatgattgat aacaactatt tttcacacaa ttctccaact
    16381 tatggttctc catttgacat gttgaaaaaa tttggaataa gctataagac agcaggtgaa
    16441 aatatagcta tgggtcaaaa aactcctaaa gaagttgtaa acgcatggat gaattctgaa
    16501 ggtcacagaa aaaatataat gaatccaaat ttcagtaaaa taggtgttgg agtagcacaa
    16561 aaaagtggtg gttcaattta ttggacacaa atctttgtag gataaaatac tctttttcca
    16621 tacatatcaa aagagtatct ctaagtgata aaaaacacaa atgagatact cttttgtatc
    16681 tattttaaaa ttatcaaatc caaatataca tactataaat attatttgtt ctcttaattt
    16741 tagtgtgtag aacaatatat aaaaagtatt ggcaagatga aaatggaaaa cttaaaaaag
    16801 atatttaata ggtaaaaaca aaatcttaat atcttcataa aaaaaggctg tctcaaaata
    16861 gagataaggc ctttttcatt gttgaaatga ttaaaatata tttgatttca tacaaaaaga
    16921 gtgcctcatg aactaaaaag ctcactttga gacactctct tttatcaatt tatataagca
    16981 ttataatact ttaatattat tcagctaatt ttttgtatcc ttcatatctt tctttagcat
    17041 cagcttcagc tttagcaaat aatccatcag cttcttctgg gaattgttta gctatagcag
    17101 aatatcttac ttgtgcaagt atgaaatctt tgaagcttgc agttggttct tttgaatcta
    17161 aagtgaatgg gtttttacct tcagctttta cttctggatt gaatctgtat aaatgccagt
    17221 atccgcaagc aacagcttgt gcttcgttag ctacagttct tcccatacct tcttttaatc
    17281 catggtttat acatggagca taagctatta ttagagatgg cccatcatgt ttttcagctt
    17341 ctataacggc tttgataaat tggtttttat cagcacccat agcaacttga gctacatata
    17401 cgtttccata tgtcatagcc atcattccta aatctttctt tctagatttt ttacctgaag
    17461 cagcaaactt agccatagca gcagctggag tagctttaga agcttgacct cctgtatttg
    17521 aatatacttc tgtatcaaat acaagtacgt ttacatcttc accagatgca agaacatggt
    17581 ctaatccacc gtaaccgata tcatatgccc aaccgtctcc acctagtatc cattgagatc
    17641 tcttaactag ataatctttt ctatctttta tagcttttac taactcttta gctttttcat
    17701 ctgtacaatc acaagctggt ttttctaata attcaacaac cttagcactt gctactttag
    17761 aagcttctcc atcatttcta gaatctaacc actctgtaaa tactgcttta gcatcttcac
    17821 agatacccat agaaactaac tctgtcatag catctactat tttatttctt atttgcttaa
    17881 cagctgtgta cataccaaat ccatattcag cattatcctc gaataaagag tttgcccaag
    17941 atggaccttt accttcatgg ttacaagtat aaggaattga tggtgcagaa gctccccata
    18001 tagaagaaca tccagtagca ttagctatca tcattctatc tccatataat tgtgtaacta
    18061 ctttaatgta tgcagtttct ccacatccag cacatgctcc tgaaaactcc attaatggtt
    18121 gtctaaattg acttccttta acagtgttta caggcataac atcacctttt ggagctacct
    18181 catcaggatt tactgcaaat gcccaattat ctaactctgt atcaagttga gtatctattg
    18241 gcttcataac taaagctttt tctttagctg gacatatgtc agcacagttt ccacatcctg
    18301 tacagtcaag tggactaagt tgcattctat attttaatcc ttctaatcct ttacctaatg
    18361 cttttttagc ttcaaatcct tctggagcat tagctaattc ttcttcagta actaatactg
    18421 gtcttataca tgaatgagga catataaaag aacattggtt acattgaata cagttatcta
    18481 ttatccattc aggaacatca acagctatac cacgtttttc ataagctgca gtaccacaag
    18541 ggaatgttcc atcttctata ccattgaatg tacttactgg taagttgttt ccttcttgtg
    18601 cagtcattgg tcttagtata ttttttataa attctggttc tttaacttct cctttatctg
    18661 catcaacagc attttcccaa gaagctggaa cttcaacttt aactaaagca tttattcctg
    18721 catcaacagc agcatagttc atgttaacta ttttttcacc ttttttacca taagctttaa
    18781 ctatagaatc ttttaagtat ttaacagcat cttcttctgg tattatttca gctaatttaa
    18841 agaatgcaga ttgcattatc atatttattc tgtttccaag acctatttct tgtcctagtt
    18901 taacagcgtt aactgtatag aattttatat catttttagc tatatattgc ttcatatgag
    18961 ctggtaaatg ttcttcaagt cctgcttggt cccatatagt gttaagtaca aatgtaccac
    19021 cttttttaag accttttaat aaatcatatt gataaacata tgattgttta tgacaggcaa
    19081 tataatcagc ttcatctatt aaataagtag ctcttattgg agtatcacca aatcttaagt
    19141 gagacatagt tataccacca gattttttag agtcataatc aaagtaagct tgagcatatt
    19201 tttcagtatg gtcaccaatt attttgatag cttgtttatt agctccaaca gtaccgtcag
    19261 aacctaatcc ccagaactta catcttatag ttcctttaga agctattttt attggctcag
    19321 atggagttaa agaagtatta gttacatcat caactatacc tactgtaaat ccatttttag
    19381 gttgttctga aactaagtta tcaaaaacag tttttatatc tgaaggagta gtatcttttg
    19441 aacctaatcc gtatcttcca cctattatca taggagcatt ttcttttcca tagaatacat
    19501 cacgtacatc taagtataat ggttcaccag ttgaacctgg ctctttagtt ctatcaagta
    19561 cacatattct ttcaacagta gatggcatag catctaagaa atgtttcatt gagaatggtc
    19621 tatataaatg aactttaact aaaccatatt tttctccctt agcatttaag taatctatag
    19681 tttcttctat tgtttctgtt actgaaccca tagctattaa tacgtattta gcatcttctg
    19741 ctccatagta atcaaataaa ctatgttttc tacctgttaa attactcatt ttttccatgt
    19801 atttttcaac tatacctact atattttgat agtatttatt agaagcttct ctagtttgga
    19861 aatatatatc tgggttttga gcagtaccac gagcaactgg atgatttgga gataaagcat
    19921 tatctctaaa tgcttgaact gcttcaaagt ttaataaact tgcataatct tcattttcta
    19981 gtaattcaac tttttggatt tcatgtgaag ttctgaatcc atcaaagaaa tgtataaatg
    20041 gtaatctacc ttctattgca gctaaatgtg caacaggtgc tatatcagct acttcttgaa
    20101 ctgaaccaga agctaataat acacatccag tttgtctagc agccataacg tcttggtgat
    20161 ccccaaatat tgataacgct tgagatgcta aagcacgagc acttacatgg aatactcctg
    20221 gtaataactc tcctgcaact ttatacatgt taggtatcat taataataaa ccttgagatg
    20281 ctgtaaaagt agaagttaaa gctcctcctt gtaaagaacc gtggaatgtt cctgatgcac
    20341 ctgcttctga ctgcatttca acaacattaa ctgtttgtcc aaatatattt tttcttcctt
    20401 gtgatgccca ctcatcaact acttcagcca tagttgaaga tggtgtgatt ggatatatag
    20461 ctgctacatc tgtaaacgca taggcaacat gagctgcagc tgtatttcca tcaagtgttt
    20521 tcataaactt agccataata tgtatcctcc ttgatagcta caattgtact tgtttataac
    20581 aagtctattt ttttatattt acatataatt tacacaacaa aactataaac acacatatag
    20641 tcgcgcaaat actctatctt ctaatataat actacagatt agataaaatt
//
LOCUS       DAYAOX010000053        20260 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.053, whole genome shotgun sequence.
ACCESSION   DAYAOX010000053 DAYAOX010000000
VERSION     DAYAOX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.053"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            19..1932
                     /locus_tag="ACGI57_001530"
     CDS             19..1932
                     /locus_tag="ACGI57_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9716611.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            2203..3177
                     /locus_tag="ACGI57_001531"
     CDS             2203..3177
                     /locus_tag="ACGI57_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HFG9716612.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            3198..3473
                     /locus_tag="ACGI57_001532"
     CDS             3198..3473
                     /locus_tag="ACGI57_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFG9716613.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            3486..4649
                     /locus_tag="ACGI57_001533"
     CDS             3486..4649
                     /locus_tag="ACGI57_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFG9716614.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VNYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            4986..7265
                     /locus_tag="ACGI57_001534"
     CDS             4986..7265
                     /locus_tag="ACGI57_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9716615.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(7360..8700)
                     /locus_tag="ACGI57_001535"
     CDS             complement(7360..8700)
                     /locus_tag="ACGI57_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9716616.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(8712..9494)
                     /locus_tag="ACGI57_001536"
     CDS             complement(8712..9494)
                     /locus_tag="ACGI57_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716617.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(9509..10531)
                     /locus_tag="ACGI57_001537"
     CDS             complement(9509..10531)
                     /locus_tag="ACGI57_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFG9716618.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(10537..11550)
                     /locus_tag="ACGI57_001538"
     CDS             complement(10537..11550)
                     /locus_tag="ACGI57_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFG9716619.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(11678..12616)
                     /locus_tag="ACGI57_001539"
     CDS             complement(11678..12616)
                     /locus_tag="ACGI57_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9716620.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLAKPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            12886..13350
                     /locus_tag="ACGI57_001540"
     CDS             12886..13350
                     /locus_tag="ACGI57_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716621.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(13462..13779)
                     /locus_tag="ACGI57_001541"
     CDS             complement(13462..13779)
                     /locus_tag="ACGI57_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9716622.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(13792..14523)
                     /gene="chbG"
                     /locus_tag="ACGI57_001542"
     CDS             complement(13792..14523)
                     /gene="chbG"
                     /locus_tag="ACGI57_001542"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFG9716623.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLLSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(14549..15859)
                     /locus_tag="ACGI57_001543"
     CDS             complement(14549..15859)
                     /locus_tag="ACGI57_001543"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFG9716624.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(15956..17269)
                     /locus_tag="ACGI57_001544"
     CDS             complement(15956..17269)
                     /locus_tag="ACGI57_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9716625.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(17291..17602)
                     /locus_tag="ACGI57_001545"
     CDS             complement(17291..17602)
                     /locus_tag="ACGI57_001545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9716626.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(17802..18506)
                     /locus_tag="ACGI57_001546"
     CDS             complement(17802..18506)
                     /locus_tag="ACGI57_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9716627.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDVRVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(18597..19958)
                     /locus_tag="ACGI57_001547"
     CDS             complement(18597..19958)
                     /locus_tag="ACGI57_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9716628.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
BASE COUNT         7128 a         3349 c         2659 g         7124 t
ORIGIN      
        1 aatatttaag tttatttgat gaaatatgga ggaattatta tgaaggaaat actcgttatt
       61 gctcccacta aaggaaccta tgaaaaatca attcacatag ttaaaaaaaa caaatataca
      121 aatattgatg ttgtatttgg caatctaaaa gaaggtatac ctcttgctga aaaaagtatt
      181 aatcatggta ctagaattat cataagccga ggtggaactt acaatatgtt aaaagctact
      241 tacaatatac ctatagttga aattaaagta gatgcttatg atattataaa aagctacaag
      301 gaggtaaaaa attccaacga accatttgga ataattggat ttaataatgt catctacggt
      361 ttcgatatta tagaagaaat attgaataag aaaataacta tgattgaaat tgaaaaagaa
      421 gaagaaattt atgatgctat agaaaagtac agaaagaaag gtataaacac atatatagga
      481 gataccactg ttgcacatat tgttaagaga cttaattgta agggaatatt aatcgaatct
      541 agagaagaaa acatacttag agccattcaa caagcagaac aaatactcga agcaaccaag
      601 gatgaacaaa aaagaagatt acagatagaa gctatgacag attttgtaca tgatggaata
      661 ataacagttg ataaaaattt taaaataaca ttatttaata agagtgctga aaaaatattt
      721 ggtataaaaa aagaaaatgc actttatcat aatgttgtag atgttatccc aaacacagtt
      781 cttccacaag tagttgagac tatggaacct caaattggtg aaatacaaaa tatagggagt
      841 acaaaaatta ttacaaatag agttccaata attgttgatg gtcagattca aggggctgtt
      901 tcaacatttc aagatagcaa ggagataagt tcttttgaac atacaataag aaggagctta
      961 ttaagcaaag gtctatctgc taaatacact tttgatgata taattcatgc tagtgaaaag
     1021 acaaaagatt gtatagaact tgcaaaaaaa tatgcttgtt atgatactcc tatgattatt
     1081 actggagaat ctggagttgg taaagaacta ttttgtcaaa gtatacataa ctatagtaag
     1141 cgtaaaaatg caccttttgt tgctgtaaat tgtgctgcca tacctccatc tctaatagag
     1201 agtgagtttt ttggttatga ggaaggttca tttacaggtg ctagaaaaaa aggtaaagca
     1261 ggtgtattcg aactagcaca tgaaggcaca atcttcctag atgaaataag tgaaatccct
     1321 cttgaacttc aaggtcgatt attgagggta ttgcaggaaa aacaagttat gagaattggt
     1381 ggtgataaag ttattcctat agatgtaaaa attatctgtg cttcaaataa agacttaaaa
     1441 aatatgatgc gcaatggttt atttagacaa gacctttatt ttagaataaa tatactaaca
     1501 ttatatcttc cttcattaaa agaaagaaaa gaagatatta taaaattatc agaatttttt
     1561 atacataaat attcaagcaa atacaataaa acgcctttaa ttatcactcc aaatattaaa
     1621 aaggcacttc ttgaacgaga atatgaaggt aatataaggg aattggaagg tatgatggaa
     1681 agggctgtta ttgtggaatc atttgactcg attttattag aaaagcttag caatgaatat
     1741 acaaataata tagttgataa agataatagt gattttgaat gcagtaattt agatttaaaa
     1801 actttagaaa aaaaatatat ctataaaatt tataacgaaa ataatagaaa tactacaaaa
     1861 acctgtgaaa ttctaaagat aactcgctcc actctatggc gtaagttaaa agaaatagaa
     1921 gatgatgttt aaaaataata catatctata tatatcaaaa cgttttatgt ttaattttaa
     1981 tacataagac gtttatttgt tttcaataaa aaatacaatt atatgctaaa aagaatcttt
     2041 gtaagtgaaa ttctcgtcaa tcatactttt aattataagt aatctacagc atttcaatcg
     2101 attattaaaa ataatcatga taactaaaga tacaaactct atatattggc atagtaattg
     2161 cttttataat acctatataa aaatgaaagg gagtgatact agatgcacat cttaaaatca
     2221 gtgaaaaaga taccaggagg acttatggtc tgtcccttgt tattgggagc actatttaat
     2281 acagtatgtc cacaagctct tgaaattgga ggattcacaa catccttatt taaaactggt
     2341 gcaatgagta tacttgctct attttgtcta tgcaatggag ctcaaatcaa tatcagacaa
     2401 gctggtaaac cattgactaa aggtatagta cttacagcaa caaaatttat aattggtgca
     2461 gtattaggta ttattgttag taaatttatt ggacctaaag gtataattgg tattacacct
     2521 cttgccatag ttgctaccat gactaattca aatggaggtc tatttgctgc tcttgcaggt
     2581 gaatatggag actccacaga tgttggagca atatctatct tatctctaaa tgatggtcct
     2641 ttttttacaa tgcttgcttt tggagtaact ggacttgcaa atgtacctat agtagcacta
     2701 tttgcagcac ttataccaat actacttgga tttatacttg gtaaccttga tgaagatata
     2761 cgagaatttc ttgcacctgg tacaacatta ttaattcctt tcttcgcttt tcctttagga
     2821 gcagcattaa actttaatca aattataact gctggactac ctggagttgt acttggttta
     2881 ggagttacta taataacagg tatgggtggg tactttgtaa tgaaacttat acgtgcagaa
     2941 catccagcag taggtgcagc tacaggtaca acagccggaa atgcagctgg tactcctgaa
     3001 gctttagcag ctatagaccc tactctagct acaatagcag ctgtttcaac agtacaagtt
     3061 gctgctgcta ttattgttac agcaatatgt tgtccaatgt tagtttccta tctagataaa
     3121 agagaaaaaa agaaaaaatc tatttttaaa acaaataaaa atattgaagt aaattaatac
     3181 ttaaggaggt aaaattaatg aatgcaatta ttcatgcaaa tgaaaaagat aatctagtta
     3241 catgtgtcag acctttaatt aaaggtgaaa aagtttcaat tgatggaaag tcttacatag
     3301 taaatgataa tatacctgtc tttcataaga tggctacaga agatgtaaaa aaaggtgatg
     3361 ttgtatacaa atacggtgaa attataggaa ttgcaacagt tgacataaaa actggtgact
     3421 atgttcatgt acataacatt gaaagtacac gtggtcgtgg agataaaaaa taggaggaag
     3481 ttaaaatgaa attaatgggt tatttaagag aaaatggtca gtttggtatt cgtaatcatg
     3541 ttttaattat tccaacatct gtatgttcaa gtgagacagc tacaagaata gcatcacttg
     3601 tgcctggagc aatagcaatc cctcatcaac atggatgttg tcaaattggt tctgatatag
     3661 agcttacagc aaagacatta ataggatttg gaaaaaatcc taatgttgcc gcagttttag
     3721 tagttggtct tggatgtgat ggtattcaag caaaagaatt agcatctgaa atagctacaa
     3781 caggaaaaaa ggtaaattac gttgttatac aagaatgtgg tggaacatta aaaacagttt
     3841 ctaagggagc tgaaatagta agtaaaatgg ctcgtgaagt atcaaaagaa gtaagagttg
     3901 aatttggtat gagtgaaatt acattagcac ttgaatgtgg tggttctgac ccaacaagtg
     3961 gaatagcttc aaacccttca ataggcgttg catcaaacct tcttgtagat gaaggtggaa
     4021 gtagcatact aagtgaaact acagaagtaa ttggtgcaga acatttgttg gctacccgtt
     4081 ttgaagatga agaaatgaaa gataagtttc ttaaatttgt aagtgatgta gaaaaaagag
     4141 caattgctat gggagaagat ttacgtagtg gtcaacctac tccaggcaat aaagctggtg
     4201 gactttcaac aattgaagaa aagtcacttg ggtgtatgta taaggcaggc aataagccat
     4261 tcaaaggtgc tcttgaatat gctgatattg tacctcctga taaaaaagga ttatatttta
     4321 tggatactcc aggtcaagat atagattcta tcactggtat ggtagcaggt ggtgcacaaa
     4381 ttgtaatctt ttcaactggt agaggtactc caacaggaag ccctatttca ccagttatta
     4441 aaatcactgg gaatagtgat acatacaaca aaatgccaga caatatcgat ataaatgcag
     4501 gtagaattat aacagatggt gctaaaatag cagatatagg tcaagaaata tttaatgaaa
     4561 taatagaagt ctgtaatggt aaacatacca aagctgaaag tcttggtcat agagaatttg
     4621 gcatttacag aatttcatct acattttaga ttgtaacaaa atatctttta taaatataat
     4681 acttatgtag atacataagc taaaagttct ttctaatact ataaaaattt tctatactga
     4741 aaaggaactg tatcaaaatt attttgaaaa cagttccttt tttaacctct tttactcaaa
     4801 atattataaa ataagattta aacaataaac gtatcttgat taatctctaa gaagacataa
     4861 ataaagctaa atgtaaagtg ggataaataa aatttaatat ctttgtatga aaagatattt
     4921 ggtataatat gtctgagaga attcttattt gtaaacaaat aaaggatgga ggatgaaaca
     4981 aaagaatgaa ctttgaaaac acaaaaaata aaaaaataat agctacttta ctattgacac
     5041 tccttttaat cgtgtcatta acatgtgtca cttttattat gaatctaaat aaagtaacac
     5101 ataatgaaac agaacgatat cttgtagaag tttcacaaag tatttccact acaattaatg
     5161 tgaaatgtaa aagtatattt aggacactat attctatggg agatacttat tttgagatta
     5221 atgatagtga aaaagaagtc aatagatact tatcaaaaaa attaaaatta tttgaatttg
     5281 attggttagg tattatagat tctaatggaa attttgtaag tgcccttaaa gatacaggta
     5341 atgttaaagg acaagacttt tatagaaaag cccttcaagg caaagaaaca attactcctg
     5401 ttaaatcaca atctggtaca tatggaattt tatattctgt accactatac aatggtgata
     5461 aaattgttgg agctgttgct gcttggaata ctattgatac tatgagaaat ttattaagtg
     5521 tagaaagctt tcatggtgaa ggattttccc aaattatcga ccaagatgga aattttattg
     5581 taagttcagc aaacaaaaat gctccaaaaa atgtggtaaa tttttttgac atgattaaac
     5641 accaaggaaa acaggttgat aaaaataaac ttgaagaaat gaaaataaac ttaaaaaaga
     5701 acaaaactgg ttctatgtat tatacattaa atgatggtat ttctaaggca atgcattatc
     5761 ttccattaca agaagaaaac ttatacctcc tttcaattgt tccaacaaaa acagcaagtg
     5821 cacagtttga tttacttcta aaaaaagcaa tcttcatcaa tattttaatt gttatactat
     5881 ttttattgct tattatattg attatgttta taaatcaaaa gaacagggca caacttataa
     5941 aaatcgccta tgtagactct gtaactaaag gttacagtaa agcacgtttt gatattgaag
     6001 ctaaaaaaat tatacagtca tcttcatcag aaagctattc attggtatct ataaatattc
     6061 agaaattcaa acttattaat gattcttttg gtagtgaagc aggagataaa acattaaagt
     6121 acatacatga tgtcattagt aattatctag aaccaggaga attgctttgt cgagtttcta
     6181 atgaccagtt taatgtactt attaaaacta ttcctcaaaa agaaattctt aagtacatag
     6241 aaaatatcac caatgatatt aatagtttca ataaaaatga agaatgtaaa tattttatag
     6301 ctctatctat aggtgtatat aaaattgatg acccaaatct acctttagtt tcaattcaag
     6361 accgtgcaaa tgttgcacga aaaaataata aaggaaacat tggaaatcat ccactttata
     6421 catgtgtttt ttatagtgat ttagagcgtt taaaaatgat taaagaaaaa gaaatggaaa
     6481 atagaatgga ggaagcatta aataataacg aatttatagt atatcttcag cctaaaataa
     6541 gcctcaaaga taatactatt gtaggtgcag aagcattagt aagatggcaa gacccaaata
     6601 aaggtcttat accaccagac gagtttattc ctttctttga aaaaaatggt tttattacaa
     6661 aactcgacca ctatgttttt gaaaaagtat gcagtatgat aagaaaatgg atagataatg
     6721 gagaaactcc aatacctgtt tcagttaatt tttcacgtgc taatttaaat aatatagatt
     6781 ttttagaaaa atacagaatg cttcgtaata aatacaacat tcctgctaag ctcttagaaa
     6841 ttgaattaac tgaaacactt gtatttgaaa atctccaaaa attaattaat ataatagacc
     6901 aaatccatga agaaggtttt caatgttcac tagatgactt tggaagtgga tactcttcac
     6961 tcaatatgtt aaaagaaata aaagttgaca cattaaaact tgaccgtgct ttttttagct
     7021 cacctaatgc agataatatt gctgaaaatc atgtaataga atctatagtt aatttggcaa
     7081 aaaaattaaa tatgaatagt atatcagaag gtgttgaaac aatcttgcaa atggattatc
     7141 ttaaaaaaat taattgtgat atgatacaag gatatgtttt ttcaaaacct ctacctcaag
     7201 aaatatttga gaaaatgact tttggaaaaa aactagaaga aaccagtatg gatagttccg
     7261 tttaaataaa ataaaaaact tgtctctata aaataatttt aatcatttta taaagacaag
     7321 tctttttatt ttccaattta attttatttt ctaaatttct tacactcggc ttgttttaaa
     7381 ttcccttttt aagaataata atacaattat catagcaata aaatctgcaa ctggctgtgc
     7441 caaccaaaca ccatctaaat taaattgaat tggaagtaca atgattaatg gtataaataa
     7501 aataaattgt cttaaaagag ttaaaaacat tgaatgttta actttactta tacactgaaa
     7561 atacacaggt cctacaatag aaatccccat tataggcaat gtaattagat ttaggcttat
     7621 acctcttact gctatatcca tcaaatctga atctttatta aatataccta caaatacttc
     7681 tgggaaaact tgtactacta aaaatccaaa tgacaatatg acaactgaca ctatgataga
     7741 taatattaaa gttttctttg cacgctcata ttgttttgca ccataattgt atgatattat
     7801 agtttgcata ccttgattca atccaaacac aggcatcata aacatcaata aaatagatgt
     7861 aagtgctgtc atagctccaa tagctaaatc tccaccataa acctttagag aattatttgt
     7921 aaacatgtga gttatactta tagctacttc catagcaaaa ggtgtcattc ctattgcaaa
     7981 taccttcttt actatatctt tattcaattt taagttacat ttttttagtt ttaaattgga
     8041 cttccctctt ataaaataat gtattaccca aaagaatact actatttgac agagtactgt
     8101 tgctatagct gctcctttta tacctaaatt aaatacaaat ataaatatag ggtccaatat
     8161 caggtttaat acacatccta caatcattgt ccttgctgct aattttggat taccttctgc
     8221 cctaattgca ttattaagag aaaatgcaac taaattaaat atacttccaa ccaatatgac
     8281 actcatatag tcttttgcat aaaatattga atctttactt gcacccaaaa taaaaagtat
     8341 atcttctaag aaaactaatc ctaatatagt tataattaaa gagataatta tagatagtgt
     8401 caaagcatta cctaatatct tctctgcttc ttctctattt ctttccccta actttataga
     8461 caatcttgtg ctagcaccta ctgataccaa cattccaaaa gctattatta gagtaaatac
     8521 tggcatagtt acaccaagac ctgcaatagc tatagagcca ataccttcca tagaaccaat
     8581 aaaagctcta tctactacat tataaagtga agttaccatc attgctatta ttgcaggtat
     8641 cgaataccta aataataaaa tccatatttt ttcatgtctt aatgcttctt gattctccat
     8701 gttgtcacca tttacaccat gtcataagtt atacaaattg gtttatttgt tcttgggtct
     8761 gttgctattt ctgcatcaat attaaataat tcttttagat tatccttcgt cataacatca
     8821 tatgcatcac cttcacaaac tattttacct ttcttaatac ctatcatgtg gtcagcaaat
     8881 cttgctgcat tattaagttc atgtattacc atgacaatag ttcttccttg ttttttattt
     8941 agttcatcta ataattttag tatctccaat tgatgtgcca aatctaaata tgttgtaggc
     9001 tcatccagta ataatatatc agtttcttgt gccaaagcca tagctatcca tgccctttgt
     9061 ctttgtcctc ctgataaatc ttccatattt ctatccttaa attctgcaat tccagtaact
     9121 tctaaactcc aatttacaat ctctctgtct tctttactag aattaccaaa tccactttta
     9181 tgaggaaatc ttccataaga aattaattct gaaacggtaa gaccactagg agcttgaggg
     9241 ctttgtggaa gtacagccat atcttttgct atttctttag acttcttttt gtagatgtcc
     9301 tctccattta tatatatttt cccactatta gggctaataa ttctacctat agttttaagt
     9361 attgtcgatt ttccacaacc attagcccct atgatggtag ttatttttcc ttttggtata
     9421 tttaaattta aattttttat aatatctgtg tttccatatg atacatttag attcttagta
     9481 tttatacagt tcataacaac accctttcct aatcatttac caacattaaa taaataaagt
     9541 acggcactcc tattatagaa actactatac ctactggtat ttctataggt gctagaagat
     9601 ttcttgatat agtatctgct attaaaagta ataatgttcc tatcaatgct gatactggta
     9661 ttaatctttt atgctttggt cctattattt ttctacctat atgtggagct attaaaccta
     9721 aaaatgaaat actacctgct actgctgtcg atacaccaga taaaattact gcatatatta
     9781 taagtcttct tctttgtttt tctacatcta caccaagact tatagatact tcatcaccca
     9841 aatttaaaac atctatatat ttagatttat aaatagttat acccataaaa atcactataa
     9901 atggaagcac agctattaca tatttccaac tggttcccca aatgcttcct gatgtccatg
     9961 ccataaccct attaaattcc tgtgttgtaa atttcaattg gaatattgtc aaaattgaag
    10021 taaaagctat gtttacacct ataccaataa gtagtaatct tgatgaattt ataccacctt
    10081 tccatgcaag agaatatata agaaatgctc caaataaagc tcctataagt gcaacaatag
    10141 gcattgtaaa tacagtcaag ttactcatac cttcatatac attaccattc ataaggtata
    10201 tataaactac tacaaacaat gcagcacctg aatttattcc caaaattcca gaatcagcta
    10261 aatcattctt tgtaactcct tgaagtatta cacctgctgt tgataatgca gaaccaacca
    10321 aaagtgctat tactattctt ggtaatctta atttaaatat tgcaatttca tgattacgct
    10381 gaccctgccc taaaaaagtc tttataacat ccattggttc tatagaaaat gagcccatat
    10441 ttaaactcaa taaaaaagtg ataaaaataa gcaccattaa aattgatata acaagtaatg
    10501 ttttagaatt tatttttata atatttttca tagtacctat ttctccccct ttctaacaag
    10561 gtatataaat attggcactc ctataagtgc tgttatagaa cctactggtg tctcatatgg
    10621 aggatttatc atcctactta atatatctgt atatacaagc aatacagctc ctaatactaa
    10681 agagctcggt attatatact tataatctga acctactatt cctttagcta tttgaggaac
    10741 tataagacct ataaaaataa tattaccaga cactgacaca gatgctccag tcattaatat
    10801 tacaagtact atacatataa atctaacaaa gttagttttt tgtcctaatc ctattgccac
    10861 ttcttctcca agacttaata tagttattct tggagccata ataatagcta aaataaatcc
    10921 aataccacca gctacaaata gcaattttat accttcccac ttcactccta caagccctcc
    10981 tgatatccag aaacttaatt gttgagatag gttaaaatac atagatattg ctgatgccaa
    11041 agaaattagc aatgtaccaa gagcgactcc tgcaagagct aattttacat tattaactct
    11101 tcctcttgat ttagaactta taaaatatat aaataatcca cttatacttg ctccaataaa
    11161 tgcaaacatc attacactaa aacttccatg tataagttga ggaagttttt tttgaagtac
    11221 cagtgatatc gaaattgcga atgtagctcc ttgtgtaatt cctataacag atggttcagc
    11281 tataggattt ctcgttattc cttgcattat tgccccagaa actgctaaaa atcctccaac
    11341 aagtgcagct gctattgccc ttggaagtct aacatccttt actattctca tatttattcc
    11401 atcttccatt tttatcaaac tatctataac agttgataga ttcatatctt ttgccccaat
    11461 agttatggat aatacaattc caaatacaag tacaactata cttactaata tcaacaaata
    11521 agttatattt tttttactca gcttagtcaa ttatcctacc tccttttccc ttttaatcgt
    11581 cagtataata tcatctatta agtattttaa ttttaagatt tattaaaaat aggtgtgaaa
    11641 aatgaatatc attatctcac acctactaag ttaagaatta ctttgttaat tcatttttaa
    11701 ctgattctaa taataactct tttccaattg gattgtatga ttgactgaaa aatggagcag
    11761 catcaagtat tgttacatta ccatctttta ctgctcttat ttgagcccag atagcactat
    11821 tttctaaatc ttttttatct gcttctgtag caattacaac tatatggtct gcatctatat
    11881 ctgttaaacc ttccatactt accattggta gtgttattcc atcctgtttt ggcatattct
    11941 taggtttagc taatttcata tcatcattga ttagagtacc tattccacca tcactaaata
    12001 ccataaattg acctgaactt gccaacactg gtaaataagt tttttctcca ttcttttcta
    12061 taacttcctt acctaacttt gtagcttttt catcatactt ctgtagccaa cttttagctt
    12121 cttcttcttt gtcaaataac tttgaaacat cagttaactt gcttctccag tcatttgcat
    12181 aatctttcat cataactaca ggagctattt cttttaattg gtcatatatt ttttcttgtc
    12241 tttgactcat tattattaaa tcaggattta cttctaatat agcttccata tccatagtat
    12301 ccatcattga gtgcccaact atctttacac ctttaagctc ttctcttatg taacttggta
    12361 atttatcagt ttcatatgag tctacatttg ctgttgcaac aggcttataa cctgctaata
    12421 atagttcttc actacttcct gatatatcaa ctattttttt aggatttgat ggtatcttaa
    12481 cctctccttt aacagattgt acaactcttg tctctgattt atctttatcc tctgtattct
    12541 tatctgaaca tgctgtaaca agtactaacg tagttataat agatataaat attgcaagtg
    12601 attttatttt tttcatgatt acttctccta cttgtttatt atacttacat gatattgatt
    12661 atcataagca catatttatt atagcatttt tctattttta gtcaatatta ttttgtaaaa
    12721 ggataaaaat tcctaattaa tcttaattat atatagctta gataaggttt tcttatatta
    12781 aacagtattt ttttaaagaa ttggacacaa atacaatgac ttttcagtta aacaatgata
    12841 aaatgcaatt tatagataat gaatttagta aggaggattc aaaacatggt acatttagta
    12901 tattgtgata atgcaggaaa aaaaggagaa aaggtcttag ataaaatctt ggctggaaca
    12961 aaaacaatgg ttattagagg tgctgctggt agaaaaatac cacatagtag agtatttgaa
    13021 gatgagttac tttattttat ggaaaaagga actgcaaaaa tatctgcaaa agccactgtt
    13081 aaaaaggtag aaaactatgt taaattaaca gaagatgaaa tttctaaaat tttaaatgaa
    13141 aatcaaccaa aattaaattt atctgaaaag caaaaagttc gttggcataa aaaatgttta
    13201 tgcttagttg aatttgaaaa tgttgaagaa ataacacctc ttgattttga ccatcaagga
    13261 aatatggatg attggcttat tgttgaaaaa attgaagatg tagttgttgg tactagtata
    13321 ccttacaatt atgagaagtc taaattttaa agtatcaata acataatact atattgctaa
    13381 actctactta aataaaaaaa gagtgcattt aataattata aaaattattt ttatacactc
    13441 ttttttaata ataattttaa attacttact ttctaatctt tcatatacat ctataaattc
    13501 ttctgctaat tgttgaaaat tcatagctgt cattaaatgg tcttgtgcat gaataagtat
    13561 aacacttatt tcagttttat taccattagc ttcattttgt atcaattctg tttggaattt
    13621 atgagcttta tgaattgatt ctttcgactt ttcaatacat tctctagctt tatctatatt
    13681 cccgctcttt gcttctttta ttgcttcaaa tgctagccct ttcgcatcac ctgcaaatcc
    13741 aattattcca aaagatatct ctatcatctt ctcatccatt tattcgacct cctaaatatc
    13801 tctatagctt gatatgacta atccattctt ttctaaaaat tctttgactt ttttagatgt
    13861 aagtatcttg tgctctttcg ttctatcaat agcataagat gtagaattta aaatataatc
    13921 atctaaaaaa gctgggtgag acataatatc cactacatca tacttcatta tttcgtctat
    13981 attttttata aaatattctt cacttacatt ttccttataa aaactatcta ttaaaggaac
    14041 tactttagaa tactctaaat tatattcaaa tccacctctt attggaagat tatacttggt
    14101 tacaatcttc tctattactg gctttaaact taataaagta tggatatgat ggtgactatc
    14161 taaatgagat atatctattc caagcccttt taccctatct atttgagcac aaagttctct
    14221 ataaatctca tcacaatcca ttttttcaat aatttcgtct gtaatcctcc taataaaaaa
    14281 accgtcttta tcaacaatag tttttaagtt agataataat ggtctaccac aacttaaagt
    14341 catatgaact ccacaattta aagttttatt ttctttaagt aagcctactc catgttctac
    14401 cccaggcata tttgccatca tagaagtaga cctaactata ccattattat gagctgaaat
    14461 tattccatag ttcacagctt cacaatatcc aaaatcatct gcatttatta taattttagt
    14521 cataccataa cctactttct atagacaact atctattaaa tctaggaaga taatctttat
    14581 gagcttccaa catttcgtct aatatagttt ttgctaagtc atcagatgga atcaatgggt
    14641 ttatacaaag tgccaaaaga gcagattcat aatcgccata tacagctgct ttagctgcca
    14701 atacttcaaa tgatttgatt tgacttacta atccatgcac agaatctggt aaatatccta
    14761 tacttattgg ttttggacca ttttttgtta ttacactact tacttctacc gcttgttcat
    14821 ctttaaaatc tcttatagaa cccttattta atgtattaac tacttggata tcttttttgt
    14881 cgttgtatat agaacttatt aaattacaag cagcatcact atagtaagcc cctcctcttt
    14941 tttcaagttg aggtggtttt acatctaatt tttcatcctt atataattca aataattgtt
    15001 cttccaattc tttaactact tgacctctag cttttccctc tttaaactct ctcaactcat
    15061 cctcaagcat ttctttagtt ttatagtagt atctatgata tggacaaggt attgcaccca
    15121 aagattttac aaattcagca ttaaaatcaa ttgcctttat attttgcata cttatatctg
    15181 ctttgacaaa tttatcaatt gcttcttttg taacatcttc tccatctaaa gatacattta
    15241 gaccataaac catatgatta agacctgcaa aatccatact tattctgtca ctttctacat
    15301 taaatagttt ggcaacatca ttttttaaat gaattggcac gttgcataga cctatatatc
    15361 ttctaaaatt agtatatttg aatacagcct ctgttattat tcctgttgga tttgtaaaat
    15421 tcaccatcca tgcatttgga cataattctt ccacatcttt tattatatca aatataactg
    15481 gtattgtgcg aagggcttta aataaacctc cagcaccatt agtttcctgc cccataactc
    15541 catgagataa aggaatagtt tcatctttta ttctcgcatc taaaagacct actctaagtt
    15601 gtgttgtcac aaagtctgca tctcttagag cttctcttct atctaaagta agatttattt
    15661 tcatatctac tccagctttt tttaccatcc tcttagctag atttccaact atctctaatt
    15721 tttcttttcc agcttctata tctacaagcc ataattcctt tacaggtaac tcttcatatc
    15781 tatttataaa accctctaca agctcaggag tataacttga acctcctcca attgtaacta
    15841 tttttagtcc ctttttcatc tcattatacc atccttacct ataatttaat atactctaca
    15901 aaaccccata catttattta aaactctata catttattta ccgaaataaa actacctagt
    15961 tgttaatttt caatttatta gcttccattt tttcagacgc tatgacaaat ggaatatata
    16021 ttaatattga taatattaaa ttaaatagtg ctaaagctcc accagtccag tcacctgtag
    16081 ccattgctcc acctaatata ggaggtgtta cccaaggtat tttaggtaaa attactggtg
    16141 ctactaatcc aaaatctatt gctaagtatg atacagcact acatattact ggtgcaagta
    16201 caaatggaat cataaatata ggatttaaaa ctattggaag tccaaatatc aatggttctc
    16261 ctatgttaaa tattgcaggt ggaccaccta atactatcat ctctttacgt cttcttccag
    16321 ctattaataa agctactatt aatcccaata ccattccaga accacctaaa tatacaaatg
    16381 catcaaagaa tgcacctgca agtacatggt atccttccgt tgctcctgct tgagccaagg
    16441 cagcattttc tccacctaat ttcataagta tagtttctgt aaaaggaagt gctatatttg
    16501 caccatgaag tccaacagtc caaagtatat gaattataaa tgcaataagc attgtagaac
    16561 ctagactatc agctacattt gatagtggtg ctcctaaata agtatttaaa atatctgtta
    16621 agaaacttcc attagaaagc atttttataa atattcctat tacagtaaat atcattatag
    16681 ttaacattcc tggtattaac tttgcaaatg atcttgaaac tgctggtgga actccatctg
    16741 gcatttttat agttaatttc tttaccttag ataatcttac aaatatctca gttgataata
    16801 atcctattaa tatagaagag aataaagctg ctgtacctaa gtaggtttgt tgtatcattc
    16861 cccatccttc aactactgta gttcctccct caggaactat tttaccaatt tgtggagcca
    16921 ttataaaaaa gatagataaa gctataagcc cagattgtaa cccatcactc tcataagatt
    16981 ttgctagaaa ataagcaaca cctattacta agaatactgc cattgtacct attgctcccc
    17041 accaaaggtc gcctccaaat gtggtccagt tttctccaaa tatacttgcc atcaaatttt
    17101 tgtatgcttc caatggtaag ttatttatca atgttcccaa tgcacctacc atagttatag
    17161 gtatcattac tataaatgca tctcttactg ctactaagtg cctttgagct cctattttag
    17221 cagctacagg cactatatat ttatccataa atgacatgaa tttttccatt ttacatcctc
    17281 cagtacaact ttattttgaa ctatttaact ctacagctct atctaacaca gcttttccat
    17341 tacatcttcc ataatccatc atatttataa cttctactgg aatattatat ggttttgcca
    17401 tttcagttgc ttttcctaat acatatctaa cttgaggccc taaaagtaag acatcacaat
    17461 tttctatttc attttttaag ttcacttctg atatagccca tatttttgct tctataccgt
    17521 tttcctttgc tgcatcctcc atttttgtaa ctaataaact agttgacatt cctgctgaac
    17581 aagctaacat tatttttatc attttttccc ctccaaatta gattcattaa atatttttta
    17641 agtcataagt tttttaaact atacatttaa aatatgtttt tctattaatt ttttcttaat
    17701 gtatatatct tatttttaaa tgtatataca tttaattctt gatattatta tataccaaat
    17761 gtatatacat ttcaagaaaa ctttttcatt ttatttaaat tttattcgac aacagcattc
    17821 aaagttaatg tatatccata gtgatatgtg tgtgagtact caaaaatctc actattttgt
    17881 aacttacaaa tttggcttat atataaaagt ggctctggtt cttctaattt tagattatcc
    17941 atacactctt cattcgatat tacagcttgt acttctctct ctgacatggc tatcttgtat
    18001 ccacacactt cttcaacaaa attataaaga gaattttcac aatgagtctt atttaaattt
    18061 ggaaataact taactggcat ataagtaatc atatatgaaa ctcttacatc attagctaat
    18121 cttactcttt ttatttccca aacattttct tctaaactta tattcaactt acttgataaa
    18181 ttttcatctg cctgaactat ttcaagagat accaatatat tagatacttt atagccttta
    18241 ctttccatct ctttagtaaa tccacttaca gacgatatat tatcatatat attatttgca
    18301 ataactatac tacctacacc tctttgtcta gttatatagc cttctttaac tagattatta
    18361 agtgcagacc ttacagtcat tctagttaca ttaaattctt cactcagctg tttttcagaa
    18421 gggattaaat ccccttcttt caaactgtct gaatttatta actctctaac atgattttct
    18481 ataactttat aaattggctc tttcatttaa ttcactcctt attaaatctt ttatataata
    18541 agataatttt atatacaaac aaagaaaatg tctatacatt taagataatt taaattttat
    18601 tcagaaatct tgtacaactt tccgagtttt aataaagata taaatagact tatcattaat
    18661 atacctttta atatacttga taaggttata ctccaccata caccatttag acccaacatt
    18721 tctggtctag atataaagta agccaatggt actcttgcac cagtcagtat agtaattata
    18781 atagaaggta tataggttct ccctaatcct ttgaaagctc ctgttgttat tatttctaaa
    18841 cacataaaaa attgagaata gcctaatatt ttaagataaa ctgctccttt ttctattgcc
    18901 tcactttcat ctataaaaat agaaaatata ctctctcctg caaacactaa tactaaagta
    18961 gttattatac caagtacaga agatacagtt atagctattt tgcaaccttt atttattcta
    19021 gaatacttct tggcaccata attttgtcca acaaaactgc ctaatgcaac tgatagccct
    19081 tcagccgtca tccaggaaat tgcttctatt tgtgagccta ctttttgtac tgcaacagcc
    19141 acaggccccc atgatgctac tattactcct aatagcattg agaatactgt aaacatacca
    19201 ctttgtattg ctataggtaa tcccaattta tacagaactt tataatattt agtctctatt
    19261 tctttaaata gtcttaattt aaaatattct tctttacttt taactattat atatataaaa
    19321 catgtagtaa ctactatctg agcaaataca gttgcaatag cagctcctgc aacaccaagt
    19381 tttggagcaa atccccatcc aaaaattaat atagggtcaa gtataatatt agttattaat
    19441 cctactgtat taatacgaaa aggagtttta ctatttccaa gaccattgaa aattgcagta
    19501 aatactggat taataaaata aaataccata cctagagcta ctattatcaa atactgtcta
    19561 gacatggtta taacttctaa gtctcctaat ctaaaaaaac ctatcaattg tttattaagt
    19621 actattaaag aaattgtata tattattgct agtataatat ttatttctat tgctgacttc
    19681 atatatgatt tagtagtgct tatattattt gcaccaatac tttgtgctac ttttatctct
    19741 ccacctactt ttgaaatcat aataaatgcc attgcaagcc atggaaaaaa accagctgtt
    19801 ccaactgcag ctactgcttt acttccggct ttaccaaccc atatcatatc tatcatgtta
    19861 taccccatct gtataaatga agtccccatt attggaagtg atagcttaag taatttttct
    19921 gtaattcttc cttctgttaa attaagtctg tctttcattt tccacctctt ttatattaaa
    19981 tttatttgct tatatagcta gattaatcat tttatttgat tgagttcact atccttcaat
    20041 ttatattatc atatctcagg attgcatctc aatcatactt atgtttaaga tacagtattt
    20101 tacctatcta tgataaaatt aatacttatg aatataaatc tagccatttc atcaatacat
    20161 attctaacct ttaacttaac ttcaattaag tatacatatc gtaaccaatt agaatatttt
    20221 atacggtatc ttccattaat aaacaaccaa tatcaatgat
//
LOCUS       DAYAOX010000054        20063 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.054, whole genome shotgun sequence.
ACCESSION   DAYAOX010000054 DAYAOX010000000
VERSION     DAYAOX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.054"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            100..384
                     /gene="groES"
                     /locus_tag="ACGI57_001548"
     CDS             100..384
                     /gene="groES"
                     /locus_tag="ACGI57_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFG9716629.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            417..2045
                     /gene="groL"
                     /locus_tag="ACGI57_001549"
     CDS             417..2045
                     /gene="groL"
                     /locus_tag="ACGI57_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFG9716630.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTESAVADLPEKEDAGMPGMGGGMPGMM"
     gene            2307..3047
                     /gene="larB"
                     /locus_tag="ACGI57_001550"
     CDS             2307..3047
                     /gene="larB"
                     /locus_tag="ACGI57_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HFG9716631.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            3182..4314
                     /gene="larC"
                     /locus_tag="ACGI57_001551"
                     /pseudo
     CDS             3182..4314
                     /gene="larC"
                     /locus_tag="ACGI57_001551"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
     regulatory      4374..4475
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            4696..6231
                     /gene="guaA"
                     /locus_tag="ACGI57_001552"
     CDS             4696..6231
                     /gene="guaA"
                     /locus_tag="ACGI57_001552"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFG9716632.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(6308..7354)
                     /locus_tag="ACGI57_001553"
     CDS             complement(6308..7354)
                     /locus_tag="ACGI57_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9716633.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRRWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGSIGKKNAD
                     EMNFWTLEEFKKFIEFEKKSEPRLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IRLNGEDIVSSPKTPKSKRVVPIPHFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LDRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(7431..7646)
                     /locus_tag="ACGI57_001554"
     CDS             complement(7431..7646)
                     /locus_tag="ACGI57_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9716634.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
     gene            7932..8063
                     /locus_tag="ACGI57_001555"
     CDS             7932..8063
                     /locus_tag="ACGI57_001555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716635.1"
                     /translation="MNENENMSLYVSKLKEVLKNNSEEYKKIRAKINEIYNLNEKKE"
     gene            complement(8080..8481)
                     /locus_tag="ACGI57_001556"
     CDS             complement(8080..8481)
                     /locus_tag="ACGI57_001556"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9716636.1"
                     /translation="MQTLAEIIKEYRSEHNLSLREFSNLCGVSHTYIDKIEKNRDPRN
                     GKAVEPTLDMLEKMSFGLNLTLKELLTKLGKIQPNTQQDDELLKKVNSKEDNEVNELI
                     NRLASLDSDDKNAIKKMIDNAYYKAVNTKKE"
     gene            8667..8858
                     /locus_tag="ACGI57_001557"
     CDS             8667..8858
                     /locus_tag="ACGI57_001557"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9716637.1"
                     /translation="MRKESRISQKKFADKIGISRPYLSRIENGKVNPSLEIAHKISLE
                     TGKTINEIFFDFTVNHSKL"
     gene            complement(9153..9371)
                     /locus_tag="ACGI57_001558"
     CDS             complement(9153..9371)
                     /locus_tag="ACGI57_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9716638.1"
                     /translation="MTKFKDNIKLVRKQMNMTQKQFASLFGISERAYQYYESGSREPN
                     IETLILISNKLNVSTDFLLGLSPNQNRN"
     gene            9484..9678
                     /locus_tag="ACGI57_001559"
     CDS             9484..9678
                     /locus_tag="ACGI57_001559"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9716639.1"
                     /translation="MKINNNLKLQREKVGLTQLEVAQKAKITERSYQYYEAGERLPNI
                     RTALKIARILNTNCEKLFNE"
     gene            9720..10484
                     /locus_tag="ACGI57_001560"
     CDS             9720..10484
                     /locus_tag="ACGI57_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFG9716640.1"
                     /translation="MNNLQLNNKNTITTLEIADMLEINHWEVLRKLEGTEKTKGIIDI
                     LNDNNFVVVDYFIKSAYLDLKNENRKCYNVTKLGCDFLANKFTGEKGIIFTARYVKRF
                     NKMEQELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQAMKPKADYFDALVERNLL
                     TNIRDTAKELGVKEKVFVSWLIEKKYCYRDSKDKLQPYANRMQYFEKKEFTNEYGYSG
                     VQTLINPRGREAIRLLLIKDGLIKEKEKGCQITLLG"
     gene            10531..10740
                     /locus_tag="ACGI57_001561"
     CDS             10531..10740
                     /locus_tag="ACGI57_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716641.1"
                     /translation="MAKAVAKEQLFYRAKDIAKFLDICEATAYKIIAELNEELEKEGF
                     KTFSGRVSVAYFKERYCYKPRKGVI"
     gene            10741..10881
                     /locus_tag="ACGI57_001562"
     CDS             10741..10881
                     /locus_tag="ACGI57_001562"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716642.1"
                     /translation="MNVKVLIAYIQFCNDKQIKASFEGLRKYNRDIELVNLYLPTHLK
                     IK"
     gene            10897..11109
                     /locus_tag="ACGI57_001563"
     CDS             10897..11109
                     /locus_tag="ACGI57_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716643.1"
                     /translation="MVFNLEEFKVGNAVRISCERFGFEIDCIVVVATEKELNLAYFDK
                     ERGCMEYQALIPEDLRYDDYILERLG"
     gene            11123..11293
                     /locus_tag="ACGI57_001564"
     CDS             11123..11293
                     /locus_tag="ACGI57_001564"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716644.1"
                     /translation="METAISIAKGQIEGANESIKELKSKENYDKESLRWWEGVKQASE
                     NILEFLEIENKA"
     gene            11375..13327
                     /locus_tag="ACGI57_001565"
     CDS             11375..13327
                     /locus_tag="ACGI57_001565"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9716645.1"
                     /translation="MKAILLKGLEIENFKGIKELRIDFNNITNIFGENATGKTSIFDA
                     FTWVMFDKDSKNRSVFEIKPLDKQNKVIRGLVTTVTAVLEVNNKEIKLTKKYEEKWTR
                     KRGESEATFTKNETTYMINDTPIKKSEYVKEIAEIADEEQFKLLTNPYFFSNELNWKK
                     AREVILEICGDITIEQIIESNKDLVLLTTEFEKENNIDKIIKNRKASKNNLSKEKEEI
                     PVRINECNKGMYNIDFEEIEVQLKAKKSDLEAIEDNLLNGTKTNEQILKDKEKIFELK
                     QETQSIKQSASKKGNKKRNELLKEKDELQYNIKNLRNNLVYLERDSELKETLRKRAIE
                     KTTNLRDKWTKKSQETLDLSVIQTECPTCKRPLDLEDIEEKKKEMLDNFNLNKAKELK
                     EIVELGKSKNDDVELFNTEIERLKVDINKTLEEIQEKAVLLDKIKKELESTKSTEIYS
                     ADEEKRLTEISVEIKELEEKINNKDNDKNIDELKESKKKLAVEIELLNKELAKRDINK
                     GLLDRKEQLLEKEKELGIELAHQEKILNLCELFIKTKVSLLESNISSKFKNVTFKLFK
                     EQINGGIEETCEALVDGVPFSNVNTAGQINGGLDIINTLSNHFGVKMPIFIDNRESVN
                     DLIDIDSQVINLIVSNDNPLKIKGVN"
     gene            13329..14198
                     /locus_tag="ACGI57_001566"
     CDS             13329..14198
                     /locus_tag="ACGI57_001566"
                     /inference="COORDINATES: protein motif:HMM:TIGR00616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFG9716646.1"
                     /translation="MANEIQRQVSVKNLLSTEAYKKRFKEVLKDKANTFMASVVNVSN
                     LPSLKDAEPNSILKSAMVAATLDLPIDPNLGFSYLVPFVNKGVKEAQFQIGYKGFIQL
                     AMRTGQYKTINAIEIYENEIKSVNRLTGEIEFNENKDEIDNEIVVGYIAYFKLLNGFE
                     KTLYMSKEDMEKYAKRYSQTYKSNKDYVVKSSLWTTDFDAMAVKTVLKRLLSKYGILS
                     IEMQKALETDQAVIKDDNSVEYVDRQVEEEIEENANKKTIDMTTEEKEKAIEIKESKP
                     EEATQVELDTPPF"
     gene            14201..14914
                     /locus_tag="ACGI57_001567"
     CDS             14201..14914
                     /locus_tag="ACGI57_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9716647.1"
                     /translation="MKIKVLGSSSRGNCYLLQLKNETLILECGVSYKEILKGLDFSLE
                     NVVGCLVTHEHKDHSKAITELTNNGIDVYSSKGTLEALKIENHRTKIIESEKLFKIGN
                     FKIVPFNTKHDAVEPLGFLINHSSFGNLLFITDSYYCEYNFNNLNHIMIECNYSKDLL
                     DNNKDKIYLRNRIVKSHFELSNVINFLKVNDLSNIKTITLLHLSEDNSDKDLFIREIE
                     KNVGVPVIVAEKGLEIYLD"
     gene            14925..15818
                     /locus_tag="ACGI57_001568"
     CDS             14925..15818
                     /locus_tag="ACGI57_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFG9716648.1"
                     /translation="MAKYRILQANFWDDGFVLDLTPEEKYFYNYLLTNGRASQCGCYE
                     LPYKIMEMQTGYNRETVEKLIKRFIEYGKIKYDSTTKEILIINWSKHNFSKSPKVRTC
                     ILKEVESIKNKEFQQYMYRVCIDYGYPIDTVSIDYGEKEKEKQKEKEKQKEKEKQKEK
                     VEPLICEEQEKNELKEFKKLYEENIGVVYPVTAEWLIEISSDVDIRLFKSAIEICAEK
                     MNMNIAYLKGILKKWKDANITTYEQLESYRLQQENKKAKKVVNGQLSKNKFANFEQTF
                     TKYTENELDNIIKKSQDAKFK"
     gene            15876..16223
                     /locus_tag="ACGI57_001569"
     CDS             15876..16223
                     /locus_tag="ACGI57_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716649.1"
                     /translation="MGKIWMDAGTFLETKDIGDIECNLREMINKNKQKNLIGRINSKG
                     QGRKSKKVECTNIITGESKIFVSAVEASEYLYFTELHITRLARTGKTTKNGWKVRYIE
                     EVSNGISKCGTSN"
     gene            16192..16329
                     /locus_tag="ACGI57_001570"
     CDS             16192..16329
                     /locus_tag="ACGI57_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716650.1"
                     /translation="MVLANVEQVIKLAEKILSKKKCSVNKAIDIAIKILNRYEYEGMI
                     K"
     gene            16326..16775
                     /locus_tag="ACGI57_001571"
     CDS             16326..16775
                     /locus_tag="ACGI57_001571"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716651.1"
                     /translation="MSLIEYRGYDFENKKWIYSKTIMWSNAVDCLLMLKEDFKWRKVC
                     NVGICSEEWAGNNQEIFEGDILKENYNPHNRSSYGIVKRDFNSIKLYLEWHYLKKFEG
                     EWIEIIDKTEIYHSRGYKVIGNEYENLEEVRKEFLERKEMLENERLS"
     gene            16759..16884
                     /locus_tag="ACGI57_001572"
     CDS             16759..16884
                     /locus_tag="ACGI57_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716652.1"
                     /translation="MNVLASLILIVGSFIAGRVYEYRLNLKEFENYDNIGGFKNE"
     gene            16877..17035
                     /locus_tag="ACGI57_001573"
     CDS             16877..17035
                     /locus_tag="ACGI57_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716653.1"
                     /translation="MNDRLEMINASVNYIQMICESSNIAIIAEHDRVRILDLETKEKY
                     DLLRSEGD"
     gene            17039..17362
                     /locus_tag="ACGI57_001574"
     CDS             17039..17362
                     /locus_tag="ACGI57_001574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716654.1"
                     /translation="MSKVIQCDFCKEIFEKYNGECIELYKKDCSKGVLGIDKHICPTC
                     YEKFIEGKIEKVENAKRFKDKLIDFLVEHEVCDCCACDCVFDSNDEKKCKAGIREWVE
                     SEVEE"
     gene            17364..17663
                     /locus_tag="ACGI57_001575"
     CDS             17364..17663
                     /locus_tag="ACGI57_001575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716655.1"
                     /translation="MAKLIVSIPLTKYLKDITKEWGETYITKTYGGHVYLSDEAPGEV
                     FKKEDDLNYIEFNDSDIVMRLPKEVHQHINLKKGCNKNLKDVLKEIKKIEEKTNI"
     gene            17770..18843
                     /locus_tag="ACGI57_001576"
     CDS             17770..18843
                     /locus_tag="ACGI57_001576"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFG9716656.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFIVGHFRGRSTRKVFPIERKSGKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGG
                     GGAKTGLYFIDLNKNSKVTINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQ
                     NGRRIKESGEPMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDS
                     QLYKQAGNAVTANVVYEIAKRMG"
     gene            18927..19622
                     /locus_tag="ACGI57_001577"
     CDS             18927..19622
                     /locus_tag="ACGI57_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716657.1"
                     /translation="MDLNKIMNDALVEIEESGFVEETVKKELEETIKRAVNEIFGSYS
                     KFRKNIEQHLGENININLDELDIQKYNLLVANVVKEKVDATMKGQGIEHLKRNLDNML
                     VGLKKEYKMSELLEELKEDKYNLDEYPCDEDCITFILESNFNSEWARWIDFDECPNVS
                     RNNCEHSILLRNDGTIAAFRYQNREISSKDIMNGFGNFGDLLFKIYAHNSKIILDLGN
                     DVDDYDLTNGEDY"
     gene            19640..19861
                     /locus_tag="ACGI57_001578"
     CDS             19640..19861
                     /locus_tag="ACGI57_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716658.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSKEMLKQKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYYCDGGIEI"
     gene            19858..>20063
                     /locus_tag="ACGI57_001579"
     CDS             19858..>20063
                     /locus_tag="ACGI57_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFG9716659.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDIEAFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQI"
BASE COUNT         8263 a         2040 c         3736 g         6024 t
ORIGIN      
        1 gtatatatat aaatctacat atataagtag tattaattta aaaataacta aaaacatagg
       61 cgaactagtc tatacttaat attttaggag gcgtgaataa tgaaaataag accattagct
      121 gacagagtag taattaaaaa agtagaagca gaagaaaaaa ctgcaagtgg aatagtttta
      181 ccaggagcag ctaaagagca acctcaaata gctgaagttg tagaagttgg accaggtgga
      241 atagttgaag ggaaagaaat aaaaatggaa ttgacagtag gagataaggt tatattccaa
      301 aaatattctg gaacagaagt taagatagaa ggacaagaat acacaatact aagacagagt
      361 gatgtattag ctgttattga ataaatatag aataaattta ttaggagggg tttaaaatgg
      421 ctaaagaaat taaattttca gaggaaacaa gaagagcttt agaagctggt gtaaataaat
      481 tagcagatac agtaaaagta acattaggac ctaaaggaag aaatgttata ttagataaaa
      541 aatttggttc tccacttata actaatgatg gagtaactat agcaaaagag atagaactag
      601 aagatagatt tgaaaatatg ggtgctcaat tagttaaaga agtagcaact aaaactaatg
      661 atgtagctgg agatggtact acaactgcta cagttttagc acaagctata ataagagaag
      721 gtttaaagaa tgtaacagca ggggctaacc caatactttt aagaaaagga atacaaaaag
      781 cagtaacagt agcagtagaa gaattaaaaa atcaatcaag aatagtagaa acacaagagg
      841 ctatatctca agttgcttct atatctgctg gtgatgaaga agttggaaaa ttaatagcag
      901 aagctatgga aatagtaggt aaagatggag ttataactgt tgaagagtct caaactatga
      961 atactgaatt agatgctgtt gaaggtatgc agtttgatag aggatttgtt tctgcatata
     1021 tggttacaga tgtagataaa atggaagcag ttttaaacga cccatatata ttaattactg
     1081 ataaaaaaat atctaacata caagaattat taccagttct tgaacaaata gttcaacaag
     1141 gtaaaaagtt attgataata gctgaagatg tagaaggtga agcattatct acattagtag
     1201 taaataaatt aagaggaaca tttgatgtag tagcagttaa agctccaggc tttggagata
     1261 gaagaaaaga aatgcttcaa gatatagcaa tacttacagg tgctcaagta atatcagaag
     1321 aattaggtta tgatttaaaa gaagctgatt tatctatgct aggtagagct tcatctgtta
     1381 aggtaactaa agaaagtact acaatagtag atggttctgg agataaaaaa gctatagaag
     1441 atagagtaac tcaaattaaa catcaagtag agcaaactac ttcagatttt gatagagaga
     1501 aattaatgga aagattagct aaacttgctg gaggagtagc tgttgtaaaa gttggagctg
     1561 ctacagaagt agagttaaaa gaaagaaagt taagaataga agatgctctt aatgcaacta
     1621 gagcagctgt agaagaagga atagtagctg gtggaggaac tgcttttgtt agtgttatac
     1681 cagcaatagg aacactaatt gagagtttag aaggagaagt taaactaggt gctcaaatag
     1741 ttaaaaaagc gttagaagag ccattaagac aaatagctat aaatgctggt cttgaaggtg
     1801 ctgtaattgt gcaaaatgtt gttaattctg aagcagaaac tggatttgac gcattgaatg
     1861 aaaaatatgt aaatatgata gaagctggta tagttgaccc aactaaagtt agtagaagtg
     1921 ctttacaaaa tgctgcatca atagcaagta ctttcttaac tactgaatct gctgttgctg
     1981 accttcctga aaaagaagat gcaggaatgc caggaatggg tggcggaatg cctggtatga
     2041 tgtaatttaa aaaatttaaa atataaattt taatataaag aactatttcg acttagattt
     2101 taaactctaa gttgaaatag ttcttttgta tatatctaat ttttgtatat ctataaattc
     2161 aatacacata tttagataat cagtatctag tttgtaataa tattttagag tagtattgtt
     2221 attttttagg ttatgatttt agatatatat taattaaggt ataattgttg atagagaata
     2281 aatcaaattt aaaaggatgc gataatatgg acttaagaaa attgcttgaa gaagttaaaa
     2341 atgaaaatat tgatatagat tttgcgttag aaaagttaaa agatttacct tatgaagacc
     2401 ttggatatgc aaatatagac catcatagag aattaagaaa tgggtatccg gaagttattt
     2461 attgtgaagg taagagtgat gagcatattt tagggattat tgataaaatg aatcaaaaaa
     2521 gttctaacat attggggaca aggtgcagaa aagaaacttt tctgaaaatt aaagaaatct
     2581 ataatcatgc tgagtacgaa gaggcgtcta aaattcttaa gattcaaaat catgatatag
     2641 agaatatagg aaaaggtaaa attgtgattg ctacaggggg aacttctgat ataccagtag
     2701 cagacgaagc ttatcataca gctaagtttt taggtaatga tgttgataga atatatgatg
     2761 ttggagtggc aggtatacat aggcttttaa ataaaagaca taaaatagat agtgctagag
     2821 taatagtagc cgttgcaggt atggaaggtg ctcttgcaag tgtaattggt ggactggttg
     2881 atgttccagt aatagctgtt ccaacttctg ttggatatgg agcaaatttt ggaggattag
     2941 cagcattgct agctatgtta aatagctgtg catctggaat atctgttgta aatattgata
     3001 atggatttgg agcaggatat ttagcatcaa tgataaataa actgtaaata cattgatata
     3061 aattaaacaa aactatataa taaaatttaa tttctattat atttttacta ttaaaattga
     3121 ggagacatat atgagtgaaa aaatacttta ttttgatata ataagcggta tatcagggga
     3181 tatgaccctt gcaagtttat taaatttagg tgtgcctaag gaaatttttt tggaggaaat
     3241 tcataaacta agaatgagcg atgaatttaa tataaacatc agctcgaaaa ctgaaaatgg
     3301 aatagtaggt acaaaagtag aagtaattac aaaagaaaaa catacacata gaaatttagt
     3361 ggatatattt gaggttatag ataaaagcac tctaaacgaa aatgtaaaat ctaaagcaaa
     3421 aaaaatattt atggtaattg gagaagcaga agcaaaagtt catggaacta caatagacaa
     3481 gatacatttt catgaggtag gggctataga ttcaattgta gatatagtag gagcttctat
     3541 attagttgac ttactttgtg tagataaagt ttgtgctagt acagtgtctg ttggttcagg
     3601 atttgtaaaa tgtgaacatg gaattatacc tatacctgca cctgcaacta tagaaatttt
     3661 aaaaggtgtg ccaattaaat taaataatgt aaatggagaa tgtacaactc caacaggagc
     3721 tgcaatcata aaagttttgt gtgatgagtt tgtagatgag cttgaatttt ctccaaagca
     3781 aattggatat ggtataggtc ataagaagtt tgaagtacca aatatactca gaactgtatt
     3841 aggtgaaaaa aaaaagaaga attaatttat gaaattagtg caaacattga tgatatgtct
     3901 tcagaaatct attcttattt gtatgaaaaa atattagatg agaaagcatt ggatatttat
     3961 acagagagca tttatatgaa gaagaataga ccagcaacaa aagtatcaat tttatgtagt
     4021 gaatcagatt tagaaaaatt tattaaaata ttacttttag aaactagtac ttttggagtg
     4081 agatataatg catataatag agaaattcta gagagaaaat ttgtaaaatt agatacaaaa
     4141 tatggagtgg ttacagtaaa actagggtat tataaaggaa aactagtcaa ggctactcca
     4201 gaatataatg aatgtaaaac tatagcaaaa aatgaagata ttcctataaa aaaagtgttt
     4261 gctgatataa attttagaat aaaagaagaa tttagtgaaa acttattgac ataagtaata
     4321 aattttgata aattaatatt cagccttatt aaataaaagt aaacagaaaa aaatatataa
     4381 aatataaact cactcgtata tgctcagaaa tatggtctga gagtctctac caagataccg
     4441 taaatatctt gactatgagt gaaattatta taccaaaaga cctagttaag taaatgtgta
     4501 ggctgagtta aactccctat gtgttggcta taagataaag gtattttgga agcctgtata
     4561 gtaatttcac ttttgctaga aattatggaa gttactatac aggttttttg tgtaaaaaga
     4621 tttaatataa tttagactgt attgtgttaa aataaatata taaaaactat attatacaag
     4681 gagagatgtg aatttatgaa acatgaatta gtacttgtaa tagattttgg aggacaatat
     4741 aatcagttga tagctagaag agttagagaa aacaacgtat attgtgaaat acttccatgt
     4801 actgcaagca tagaaagaat aaaagaaaaa aatcctaaag ggataatatt tacaggcggt
     4861 ccaaatagtg cgtatttaga agattctcca acaatatcaa aagaaatatt tgaattagga
     4921 gtaccaatac ttggaatatg ctatggtatt caaataatgt ctcatgtatt gggtggagtt
     4981 gtaagaaaag gtaataaaca agaaaaagaa tatggaaaaa cagctataac ttatggaaaa
     5041 tcatcattat ttgaaggaat aactacaaat agtgtatgga tgagtcatac agatttaata
     5101 gaaaaagttc cagaaggatt tactatagtt gcaaatacta atgattgtcc agtagcagct
     5161 atggaaaatg tagaacgaaa cctatatgga gttcaattcc atccagaggt ggaacactgt
     5221 ttggaagggg ataagatact tacaaacttc ttatataata tttgtaaagt aaaaggtgac
     5281 tggactacag attcattcat agaagataaa ataaaagaat taaaagaaaa aataggcgat
     5341 aaaaaagctt tatgtgcttt aagtggagga gttgattctt ctgttgctgc agttcttatt
     5401 cataaagcaa ttggggacaa cttaacttgt atatttgttg accatggttt acttagaaaa
     5461 aatgaaggaa atgatgtaga aagaatattt agagagaaat ttgatataaa tttaataaga
     5521 gttaatgcag aagatagatt cttatctaaa ttaaaaggag tttctgaacc agaagctaag
     5581 agaaaaataa taggtgaaga atttataaga gtatttgaag aagaatcaaa taaattagga
     5641 aaaatggatt tcttagtaca aggaacaatc tatccagatg ttatagaaag tggacatgga
     5701 aatgctgcta ctataaagtc tcatcataat gttggtggaa tcccagaaga tgtagacttc
     5761 caagaaattg tagaaccatt aagagagtta tttaaggatg aagttagaaa aataggatta
     5821 gagcttggta tagaagaagg attaatattt agacatcctt tcccaggacc aggtctagga
     5881 ataagagtta taggtgatgt aactaaagaa aaatgtgata tattaagaga agctgatgct
     5941 gtttatatgg acgagcttag aaaagcaggt ttatacagag aaatatggca agcttttgca
     6001 acattaccag atgttaagac tgttggagtt atgggtgatg aaagaacata tgcttattta
     6061 gttggtttaa gagcagttac ttcttctgat ggaatgactt ctgactggta taagatgcct
     6121 tatgatgtat tagaaagaat ttctaataga ataataaatg aagttgatgg agtaaataga
     6181 gtagtttatg atataacttc taaacctcct ggaactattg aatgggaata gtaaataaaa
     6241 aagtcgtaag ccttgtattt tcaaggtttg cgacttttaa aaaaatcttt gtgatactaa
     6301 aatgatacta tatatttaaa ttatctagtt tttgggcaac ttcaagttgc ttatttgggt
     6361 ataaatgtga atatgtattc caagttgttt ctactttttc atgccccaat ctttctgcta
     6421 tagttaatat atttacatcc atatttacca ataatgacgc atgagaatgt ctcaaatcat
     6481 gcactctaat tctttttaca ttagataact tacagcatct atctagctct ttagaaagat
     6541 aacttttagc aaatttaaat attctttcat tatcttttaa gtcatatagc ttagatagat
     6601 aatcttttat attattatat aagaaatgtg ggatagggac aacacgttta cttttaggag
     6661 ttttaggaga agaaacaata tcctctccat ttagtcttat gtagcttttt tcgacgctta
     6721 ttttattttc gaaaatatct tttggagtaa gagctaacaa ctcccctagt ctaagacctg
     6781 tccaaaataa aatttcaaat gctagtctag gttctgattt cttttcaaat tcaataaatt
     6841 ttttaaactc ttctaatgtc caaaagttca tttcatctgc gtttttttta ccgatagaac
     6901 ctgccaaatg agcaggatta gaaggtaagt tataatattt aactgcataa tttaatacag
     6961 caacaagctg gttgtttatc gttttaatat atgtttggct ataatcactt tttaataatt
     7021 cgttttgcca tcttcttata tgtgttgcag taatttcatt tatttttaga cttttaaaaa
     7081 aaggtaaaat tctaaagttt attaaatact ttttagtttc taatgtagat aattttaatc
     7141 tagaagacat atcgtgcatg tattcttcta tgagactttc aaaactcata tcagtactca
     7201 ttttagattt attcaaaaat tctctttcaa actctagagc ttctttttta gttttaaaac
     7261 ctctcttaat tttctttttc ctatccccat cgaagtctgt aaaataaaag ctagcatacc
     7321 aactttttct ttgttcgtct ttgtaagcag gcattttaga cacccccaat tatgtcattt
     7381 taatattaca ataattataa catgttagat atgctatcta aataattgtt ttattttcct
     7441 cttcttttta tttcttgaag cttgtaatat ttacacagtt ctaagaagtc ttcttctagt
     7501 gctatagata agtctaatat cgtagatata cctatatctt tgtactcttg attttctagt
     7561 tttgatatat aacttctgtt acgtctcatt ttttctgcta attctagttg tgtcattctt
     7621 ttcttttttc ttaatttttt caacatttat taaaatccac cttttaatta tttttatgac
     7681 atttttataa ttaatagttt gtgtaatttc ttgtaaaaaa tgttcctcat tagaacattt
     7741 tttggtggaa aaatgtgtta aaatgtaagt aagaaaagca ctttcaaagc actataaaaa
     7801 tagtgctgaa atagataaac aaataaaagt aataaggagg aaagatagat tctagaggga
     7861 aatcattatc atttttaaga acgtatgttc ggttggtggg atagaaaaat actttacagg
     7921 ggatggttta tttgaatgag aatgaaaata tgagtttata tgtcagtaag ttaaaagaag
     7981 tattgaaaaa taattcagaa gagtataaaa aaataagagc taaaataaat gaaatttata
     8041 atctaaatga aaagaaagaa taaaagggct ttagctcttt tattctttct tagtattaac
     8101 tgctttataa taagcattat ctatcatttt ttttattgca tttttatcat ctgaatctaa
     8161 agatgctaat ctatttatta gttcgttaac ttcattatct tcttttgagt taactttttt
     8221 taataattca tcatcttgtt gagtgttagg ttgtatttta cctaatttag ttaatagctc
     8281 ctttaaagtt aaatttaatc caaaactcat tttttcaagc atatctaaag taggttcaac
     8341 agctttacca ttccttgggt ctctgttttt ttctatctta tctatatatg tatggctcac
     8401 accacaaaga ttagaaaatt ctctaagaga taagttatgt tcacttctat attctttaat
     8461 aatttctgct aatgtttgca taatttcaca cctcatctaa gtaatagttg acttaattat
     8521 atcagaaaaa tgtaaactat tgttaacaaa aataaaaatt tagtaaaaaa taattgacaa
     8581 gagtaaaaaa aagacgtata ctatgattaa caaaaaggtt ataaataaag ttaggaggga
     8641 ttaatataaa aaataattta caatatatta gaaaagaatc tagaatatca caaaaaaaat
     8701 tcgctgataa gataggtata tcaagaccgt atttatctag aatagaaaac ggtaaggtta
     8761 atccgagttt agaaatagcg cataaaatct cgctcgaaac agggaaaaca ataaatgaaa
     8821 ttttttttga ttttactgta aatcatagta aactataagg ttttaattgt accttgaaaa
     8881 ctaaatataa aatattttaa aggagcaagt atatgaaaaa taaagaaaaa gaaataatca
     8941 ttagaggaat tttaatagga attttttatt ttttaggaat tgtatttagt aatacttttt
     9001 ttaaataata aatagaaggg gtaggtgaaa taccaactcc ccaaatcaaa ataacacttt
     9061 ggaagttaaa tatagaatat tcaaaagagg tgattagatg gaaatagagc aaacaacaat
     9121 acgcctgcta aaaagcagac gaaagttgaa tattaatttc tattttggtt aggcgaaaga
     9181 cctaacaaaa aatctgtaga tacattaagt ttgttagaaa ttaatattaa agtttctata
     9241 tttggctctc ttgaacccga ttcataatat tgatatgcac gttctgagat accaaacaaa
     9301 ctagcaaatt gcttttgagt catattcatt tgctttctta ctagcttaat attatctttg
     9361 aatttagtca taactacacg ctccaataaa aaaataaata aatttcttga cacgaacaat
     9421 attagcgtgt aatatataaa acatagcacg aacaatattg gcgtgttaaa taaaaaggag
     9481 agggtgaaaa taaataacaa tttaaaacta caacgtgaaa aagttggctt aacgcaatta
     9541 gaggttgctc aaaaagccaa aataacagag agaagctatc aatattatga agctggcgaa
     9601 cgtctcccaa acattcgtac agctttaaag atagctagaa ttttaaatac taattgtgaa
     9661 aaactcttta atgaataata gcataaaagg actcagatta caagaaggga gctttagata
     9721 tgaataattt gcaacttaac aataaaaata caataacaac attagaaatt gctgatatgt
     9781 tagaaattaa ccattgggaa gtattgagaa aattagaagg cacagaaaaa acaaaaggaa
     9841 ttattgatat tcttaacgac aacaattttg ttgtggttga ttactttata aaatcagctt
     9901 atttagattt aaaaaatgaa aatagaaaat gttacaatgt aacgaaatta ggctgtgact
     9961 ttttagcaaa taaatttact ggagaaaaag gaattatttt tacagctaga tatgtaaaaa
    10021 gatttaataa aatggagcaa gagttaaaag aacaacaacc taaattacca actacatata
    10081 aagaagcatt gcaacagtta ttaatagaag ttgaagaaaa agagcaatta caattagaga
    10141 accaggcaat gaaaccaaaa gcagattact ttgatgcttt agtagaaaga aacttgttaa
    10201 ctaatataag agatacagca aaagaacttg gagttaaaga aaaagtattt gtatcatggt
    10261 taatagaaaa gaaatattgt tatagagatt caaaagataa gttacaacca tatgcaaata
    10321 gaatgcaata cttcgagaaa aaggaattta caaatgagta tggttattca ggtgtacaaa
    10381 ctttgattaa cccaagaggt agggaagcta tcagattatt gcttataaag gatggattaa
    10441 ttaaagaaaa agaaaaggga tgccaaataa ctttattagg ttagaacttt gaaaactaaa
    10501 tacagaatat tttgaaagta ggtgtattgt atggcaaaag cagtagctaa agaacaattg
    10561 ttttataggg caaaagatat agctaaattt ttagatatat gcgaagcaac tgcatacaaa
    10621 ataattgcag aattaaatga agaattagaa aaagaaggtt ttaagacttt ttctggcagg
    10681 gtatcagttg cttattttaa agaaagatat tgttataagc caagaaaggg ggtgatttag
    10741 ttgaatgtaa aggtactaat agcttatata cagttttgca atgataagca aataaaagca
    10801 agttttgaag gtcttagaaa atacaacaga gatatagagt tagttaactt atatttacca
    10861 acacatttaa aaataaagta ggaggtttaa ttgtatatgg tatttaattt agaggagttc
    10921 aaagtaggaa atgcagtaag aataagttgt gaaagatttg gttttgaaat tgattgtatt
    10981 gtagtagtag caactgagaa ggaactaaat ttagcttact ttgataaaga aagaggttgt
    11041 atggagtatc aagcattgat accagaagac cttagatatg acgattatat tcttgaaaga
    11101 ttaggttagg gggtgtattt taatggaaac agctatatct attgcaaaag gtcaaataga
    11161 aggagcgaat gagagtataa aggaattaaa atcaaaagaa aattacgata aagaaagttt
    11221 aagatggtgg gaaggtgtta agcaagctag tgaaaatata ctagagtttt tagaaataga
    11281 aaataaggca taagaaaaga gccattacga ttggctcaat tcaaaaacat aataaaattt
    11341 aattagctat attatagcac aagggggaac taaaatgaaa gcaattttat taaaaggttt
    11401 agaaatagaa aatttcaaag gaataaaaga acttcgcata gattttaaca acataactaa
    11461 tatatttgga gaaaatgcaa caggaaaaac aagcatattt gatgctttta cgtgggtaat
    11521 gtttgacaag gatagtaaga atagaagtgt attcgagata aagcctttag ataagcaaaa
    11581 taaagtcatt agagggcttg taacaactgt tacagcagta ctggaagtta ataataaaga
    11641 gattaaatta actaagaaat acgaagaaaa atggactaga aaaagagggg aatcagaagc
    11701 aacttttact aagaatgaaa caacatatat gataaatgat actcctatta aaaaatctga
    11761 atatgtaaaa gagatagctg aaatagctga cgaggaacag tttaaattac ttactaatcc
    11821 atactttttt tcaaatgagc tgaactggaa aaaggctaga gaagtaattt tagagatatg
    11881 tggagatata acaatagaac agattataga gtcaaataaa gacttagttt tacttactac
    11941 agagtttgaa aaggaaaata acatagataa gattattaaa aatagaaaag ctagtaaaaa
    12001 taacttatct aaggaaaaag aagaaatacc agttcgtata aacgaatgta acaaaggtat
    12061 gtacaatata gattttgagg aaatagaggt tcaattaaaa gctaagaagt ctgatttaga
    12121 agctattgag gataatcttt taaatggtac aaagacaaat gaacagattt taaaagataa
    12181 agaaaagata tttgaactaa agcaagagac acaaagtatt aaacaatcag cgagtaaaaa
    12241 aggaaataag aaaaggaatg agctgttaaa agaaaaggat gagttgcaat ataatattaa
    12301 aaatttaaga aataatcttg tttatttaga aagggatagt gagttaaaag aaactttgag
    12361 aaaaagagcg attgaaaaaa caacaaattt aagagataaa tggactaaaa agagtcagga
    12421 aacattagat ttaagcgtta ttcagactga gtgtccaact tgcaaaagac ctcttgactt
    12481 agaagatata gaagaaaaga aaaaggaaat gttagataat tttaatctaa ataaagctaa
    12541 agaactaaaa gaaatagtag aactagggaa atctaaaaat gatgatgtag aattatttaa
    12601 tacagaaatt gaaagattaa aagtagatat aaataagact ttagaagaga tacaagaaaa
    12661 agcagtatta ttagataaaa taaaaaaaga attagaaagt acaaaatcta cagagatata
    12721 ttcagcagat gaggaaaaaa gattaacaga aattagtgta gaaataaaag agctagaaga
    12781 aaaaataaac aataaagata atgataaaaa cattgatgaa ctaaaagaaa gtaagaaaaa
    12841 actagctgtt gaaattgagt tacttaataa agaactagct aaaagagata ttaacaaagg
    12901 actattagat agaaaagagc agttattaga aaaagaaaaa gagctaggta tagaactagc
    12961 tcatcaagaa aaaatactca acttatgcga attatttata aaaactaaag ttagtttatt
    13021 agaaagtaat ataagtagta agtttaagaa tgtaacattt aaattattca aagagcaaat
    13081 aaatggaggt attgaagaaa cttgtgaggc attagtggat ggagtaccat tttcaaatgt
    13141 taatacagca ggacaaataa atggaggact agatataata aatactttat ctaatcattt
    13201 tggagttaaa atgccaatat ttattgataa cagggagagt gtgaatgatt tgattgatat
    13261 tgatagtcaa gtgataaatt tgattgtaag taatgataat ccattaaaaa taaaaggagt
    13321 gaattaatat ggcaaatgaa atacaaagac aagtaagcgt taaaaatcta ttatctacag
    13381 aagcgtataa gaaaagattt aaagaggtat taaaagataa ggctaataca tttatggcat
    13441 cagtggtgaa tgtatctaac ttgccaagcc taaaggatgc cgaacctaat tcgattttaa
    13501 aatctgccat ggttgcagca acattagatt taccaataga cccaaattta ggcttctctt
    13561 atctagtacc ttttgtaaac aaaggagtta aagaagcgca atttcaaata ggatacaaag
    13621 ggtttataca actagctatg aggactggac agtataaaac aattaatgca atagaaatct
    13681 atgagaatga gattaaaagt gttaacagac taactggaga aatagaattt aatgaaaata
    13741 aagacgagat agacaatgag atagtcgttg gatatatagc atattttaaa ctgctaaatg
    13801 ggtttgaaaa aactttatat atgagtaaag aagatatgga aaagtatgct aaaagatata
    13861 gccagacata caaaagcaat aaggattatg ttgtaaaatc aagtctttgg acaactgatt
    13921 ttgatgctat ggcagttaaa acggtactta aaaggttgtt atctaaatac gggatattaa
    13981 gtatagaaat gcagaaagct ttagagactg accaagcagt tataaaggat gataattcag
    14041 ttgaatatgt agacagacaa gtagaagaag aaatagaaga aaatgctaat aaaaagacta
    14101 tagatatgac aacagaagaa aaggaaaaag ctattgaaat taaagaatct aagcccgaag
    14161 aagcaaccca agttgaattg gatacaccac cattttaagg atgaaaatca aagtattagg
    14221 gagcagtagc agaggtaatt gctacttgct ccaactgaaa aatgagacat taattttaga
    14281 gtgtggagtc agttacaaag agatattaaa aggcttagat ttcagtttag aaaatgttgt
    14341 aggatgtttg gtcacacatg aacataaaga ccactctaaa gctatcacag agcttacaaa
    14401 taatggaata gatgtatatt ctagcaaggg tacattagaa gctttaaaaa tagaaaatca
    14461 tagaactaag attatagaaa gcgagaaatt attcaagata ggtaacttta agatagtgcc
    14521 atttaatact aagcatgatg cggtagagcc attaggattt ttaataaatc atagtagttt
    14581 tggtaaccta ttatttataa ctgatagtta ttattgtgaa tacaacttta ataatttaaa
    14641 tcacatcatg attgagtgta actatagtaa agatttatta gataacaaca aagataagat
    14701 ttatttgaga aatagaatag tcaaaagtca ttttgaatta agtaatgtga ttaattttct
    14761 aaaagtaaat gatttaagta atataaaaac aattacattg cttcatctaa gtgaagataa
    14821 cagtgataaa gatttattta taagagagat agaaaagaat gtgggagtac cagtaatagt
    14881 agctgaaaaa ggattagaaa tttatttaga ctagaggtga taaagtggca aaatatagaa
    14941 ttttacaagc taacttttgg gatgatggat ttgtattaga tttaactcct gaagaaaagt
    15001 acttttataa ctatctttta actaatggta gagcaagtca atgtgggtgt tatgaattac
    15061 cctataaaat tatggaaatg caaacaggat ataacaggga aactgtagag aagttaatta
    15121 aaaggtttat agagtatgga aaaataaaat atgactctac tactaaagag atattaataa
    15181 ttaattggag taaacataat ttttctaaaa gtccaaaagt acgtacttgt atcttaaaag
    15241 aggttgaatc aattaaaaat aaagaatttc agcagtatat gtatagagta tgtatagact
    15301 atggataccc tatcgataca gtatctatag actatgggga aaaagaaaaa gaaaaacaaa
    15361 aagaaaaaga aaaacaaaaa gaaaaagaaa aacaaaaaga aaaagtagaa ccgttaattt
    15421 gtgaagaaca ggaaaaaaat gagttaaaag aatttaagaa gctatatgaa gaaaatatag
    15481 gagtggtata tccagttaca gcagaatggt taatagaaat ttctagtgat gtagatataa
    15541 gattatttaa atctgctata gagatatgtg ctgaaaagat gaatatgaat atagcatact
    15601 taaaaggtat ccttaaaaag tggaaggatg cgaatataac tacatatgaa caactagaat
    15661 catatagatt acagcaagaa aataagaaag caaaaaaagt agttaatggc caactaagta
    15721 aaaataagtt tgccaacttt gaacaaacat ttactaagta tacagagaat gagcttgata
    15781 acataattaa gaaaagtcaa gatgctaagt tcaaataaaa ttaaacttct aggaagtaaa
    15841 tatcaatata ttgcttccta gaaagggggt agaatatggg taaaatttgg atggatgcag
    15901 gaacattttt ggaaactaaa gatataggag atattgaatg taatctaaga gaaatgataa
    15961 ataaaaataa acaaaaaaat ttaattggta gaataaattc caaaggacaa ggaagaaaaa
    16021 gtaagaaagt agagtgtact aacatcatta ctggagagag taaaatattt gtcagtgctg
    16081 tagaagcaag tgaatattta tatttcacgg aacttcatat tactcgtcta gcaagaacag
    16141 gaaagactac taaaaatggt tggaaagtta gatatattga agaggtgtca aatggtatta
    16201 gcaaatgtgg aacaagtaat taagttagct gaaaagatat taagtaagaa aaagtgttct
    16261 gttaataaag ctattgatat agctataaaa atattaaata gatacgaata tgaggggatg
    16321 attaaatgag tctaattgaa tatagaggtt atgattttga aaataaaaag tggatttatt
    16381 ctaaaacaat aatgtggagt aatgcagtag actgtttatt aatgctgaaa gaagatttta
    16441 aatggcgaaa agtttgtaat gttggaatat gttctgaaga atgggctgga aataatcaag
    16501 aaatttttga aggggatata ttaaaagaaa attataatcc tcacaataga agttcatatg
    16561 gaattgtaaa aagagatttt aatagtatta agttatattt ggagtggcat tatttaaaga
    16621 agtttgaagg agaatggata gagattatag ataaaacaga aatatatcat agtagaggtt
    16681 acaaagtaat tggtaatgaa tatgagaact tagaggaagt taggaaagag tttttagaac
    16741 gcaaggagat gttggaaaat gaacgtctta gctagtttaa tacttatagt aggaagtttt
    16801 atagctggta gggtttatga gtatagattg aatctaaaag agtttgaaaa ttatgataat
    16861 ataggaggtt ttaagaatga atgatagatt agaaatgata aatgcttctg taaattatat
    16921 acagatgata tgtgaaagtt caaatatagc tattatagca gagcatgacc gagttagaat
    16981 attggattta gaaactaaag aaaagtatga cctattaaga agtgaggggg attaaaaaat
    17041 gagtaaagtt atacaatgtg atttctgtaa ggaaatattt gaaaaatata atggagaatg
    17101 tattgagcta tataaaaagg attgtagcaa aggggtgctt gggatagata aacacatatg
    17161 cccaacttgt tatgaaaaat tcattgaagg aaaaatagag aaagtagaaa atgctaaaag
    17221 atttaaggat aaattaatag acttcttggt tgagcatgag gtttgtgact gttgtgcttg
    17281 cgactgtgtg tttgattcta atgacgaaaa aaaatgcaag gcaggaatta gagagtgggt
    17341 tgaaagtgag gtagaagaat aatatggcaa aattaatagt tagcatacct ttaactaaat
    17401 atttaaaaga tataactaaa gaatggggag aaacttatat aactaaaaca tatggtgggc
    17461 atgtctattt aagtgatgaa gctccaggag aagtatttaa aaaagaagat gatttaaatt
    17521 atatagagtt taacgattct gatattgtca tgagattacc taaagaagta catcaacata
    17581 taaatttgaa aaaaggttgc aacaagaatc ttaaagatgt attaaaagaa ataaaaaaga
    17641 tagaagagaa aacaaatata tagaaaatat ctaattaaaa cagtttagag agttgcaaaa
    17701 tatcttttag tataatttat tgttgaagtg ttttgtgact ctcaaaaatg aaataaagga
    17761 ggcgttgtat tgcttacatt tttagattta ttcgcaggga taggtggctt taggctaggg
    17821 atggaaaaag caggacataa atgtttggga cattgcgaat atgataaatt cgcaaattta
    17881 agttataatg ccatgcacaa accgaaggag gatgaatggt ttgaaagaga tattagagaa
    17941 attagaacag aaaatatccc aagagcagat gtctggtgtt ttggattccc atgtcaagac
    18001 atttctgttg cagggaaaca atttggattc agaggagaac gttcaagttt attttttaca
    18061 gttacaaaac ttattagaga actcaaagaa gaagatagac ccaagtattt acttattgaa
    18121 aacgttaaaa atctacttag tgttaatgga ggatttgatt tcctcaaagt tctcgttgaa
    18181 ctggatgaaa tcggctatga tgcagagtgg caagttctta attctaaaaa cttcggagta
    18241 ccccaaaata gagaacgaat attcattgtt ggacatttta gaggacgaag tacacgaaaa
    18301 gtatttccta tcgaaagaaa aagtggaaaa aatcttgagc aactaaataa tccaactcat
    18361 agtacaaata gaatttatga tgcagttgga attgctagat gtattagaag tcaggcagga
    18421 ggtggaggtg ctaaaacagg tctatacttt atagacttaa ataaaaactc taaagtaaca
    18481 ataaatgcta gatgccttaa agcaaaatat aatgcaggtg tgacaaatag aaattgtgat
    18541 aatagtggag ttttagttaa tgcagtttta acgcccgata gggtaaataa aagacaaaat
    18601 ggtcgtagaa ttaaagaaag cggagaacca atgttcacat tgacagctca agataaacat
    18661 ggaattttga aaaatggaga tataagaagg ttaacaccaa aggaatgctt taggttgcaa
    18721 ggatttccgg ataaatatta cgaaagagca gcaagtgtat gctcagatag tcaactgtac
    18781 aagcaagcag gaaatgctgt tactgcaaat gttgtatatg aaatagcaaa aagaatgggc
    18841 taaaagttgc aaaatgtctt ttagtatgaa tatttttgaa gtgttttgta actctcaaaa
    18901 atgaaaataa ggggtgggat aataaaatgg atttaaataa aattatgaat gatgcactag
    18961 tggaaataga agaaagtggc tttgtagagg aaacagttaa aaaagaatta gaagaaacaa
    19021 taaaaagagc tgtgaatgaa atctttggaa gctatagtaa attcagaaaa aatattgaac
    19081 aacatttagg tgaaaatata aatataaatt tagatgaatt agatatacaa aaatacaatt
    19141 tacttgtggc aaatgttgta aaagaaaagg tagatgctac gatgaagggg caaggtattg
    19201 agcatttaaa aagaaacttg gataacatgc ttgtaggtct taagaaagaa tacaaaatga
    19261 gtgaattatt agaagaattg aaagaagata aatacaacct tgacgagtac ccatgtgatg
    19321 aagactgtat aacatttatc cttgaatcta attttaattc agaatgggca agatggatag
    19381 actttgatga gtgtcctaat gtaagcagaa ataattgtga acattctata ctattaagaa
    19441 acgatggaac tattgcagca tttagatacc aaaacagaga aatatcatca aaagatataa
    19501 tgaacggatt tggtaatttt ggggatttat tatttaagat atatgcacat aattcaaaaa
    19561 ttatcttaga tttagggaat gatgtagacg attatgactt aacaaatgga gaagattatt
    19621 aaaatagggg atggaacaaa tgtcaaagta tgtattgaga tggcaaaaag gactattact
    19681 agatgaacgc aagataaatt attcatgtgg aagtaaagaa atgttaaaac aaaaagcaga
    19741 actattagct aaagatgaca agatactgct tgtaacaata gataaagttg aagaagttat
    19801 aaaagatact agaagtcaaa aaatggctga atattattgt gatggaggaa ttgaaatatg
    19861 ataatacaca aatttataat acatgtttta gataagaata gcgatacacc aatactaaat
    19921 gattttgagg gtagggttag tcaagatatt gaagctttct ttcaaaagaa aataagcaaa
    19981 gtatcaagag ataatgacat cagaacagca gtatttaatg actatagtaa caatctaatt
    20041 aagaagtgtt gtgaacaaat tat
//
LOCUS       DAYAOX010000055        19804 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.055, whole genome shotgun sequence.
ACCESSION   DAYAOX010000055 DAYAOX010000000
VERSION     DAYAOX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.055"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            175..966
                     /gene="pxpA"
                     /locus_tag="ACGI57_001580"
     CDS             175..966
                     /gene="pxpA"
                     /locus_tag="ACGI57_001580"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFG9716660.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            995..2215
                     /locus_tag="ACGI57_001581"
     CDS             995..2215
                     /locus_tag="ACGI57_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFG9716661.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2235..2930
                     /gene="pxpB"
                     /locus_tag="ACGI57_001582"
     CDS             2235..2930
                     /gene="pxpB"
                     /locus_tag="ACGI57_001582"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFG9716662.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            2960..3946
                     /locus_tag="ACGI57_001583"
     CDS             2960..3946
                     /locus_tag="ACGI57_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFG9716663.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGTRAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISEGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4160..4726)
                     /locus_tag="ACGI57_001584"
     CDS             complement(4160..4726)
                     /locus_tag="ACGI57_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9716664.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5078..6145
                     /locus_tag="ACGI57_001585"
     CDS             5078..6145
                     /locus_tag="ACGI57_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HFG9716665.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6164..7324
                     /locus_tag="ACGI57_001586"
     CDS             6164..7324
                     /locus_tag="ACGI57_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFG9716666.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVATILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7337..9433
                     /locus_tag="ACGI57_001587"
     CDS             7337..9433
                     /locus_tag="ACGI57_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HFG9716667.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNGLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9592..9936)
                     /locus_tag="ACGI57_001588"
     CDS             complement(9592..9936)
                     /locus_tag="ACGI57_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFG9716668.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            10228..11118
                     /locus_tag="ACGI57_001589"
     CDS             10228..11118
                     /locus_tag="ACGI57_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9716669.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEIIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            11336..12622
                     /locus_tag="ACGI57_001590"
     CDS             11336..12622
                     /locus_tag="ACGI57_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFG9716670.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(12711..13154)
                     /locus_tag="ACGI57_001591"
     CDS             complement(12711..13154)
                     /locus_tag="ACGI57_001591"
                     /inference="COORDINATES: protein motif:HMM:NF025547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HFG9716671.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(13178..13972)
                     /locus_tag="ACGI57_001592"
     CDS             complement(13178..13972)
                     /locus_tag="ACGI57_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HFG9716672.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(14537..15013)
                     /locus_tag="ACGI57_001593"
     CDS             complement(14537..15013)
                     /locus_tag="ACGI57_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFG9716673.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSIPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(15114..16283)
                     /locus_tag="ACGI57_001594"
     CDS             complement(15114..16283)
                     /locus_tag="ACGI57_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9716674.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNSNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            16446..17309
                     /locus_tag="ACGI57_001595"
     CDS             16446..17309
                     /locus_tag="ACGI57_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFG9716675.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            17380..17973
                     /locus_tag="ACGI57_001596"
     CDS             17380..17973
                     /locus_tag="ACGI57_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HFG9716676.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(18224..18859)
                     /locus_tag="ACGI57_001597"
     CDS             complement(18224..18859)
                     /locus_tag="ACGI57_001597"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFG9716677.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(18939..19670)
                     /locus_tag="ACGI57_001598"
     CDS             complement(18939..19670)
                     /locus_tag="ACGI57_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFG9716678.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKGVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
BASE COUNT         7511 a         2327 c         3310 g         6656 t
ORIGIN      
        1 attgcaaaaa tattccttaa atggtaaaat tatattaatt aaatgcaata gaatagttgc
       61 aaaaataaaa ttgaatacaa gtgataaaat atttcatgca atatataaaa taaacaaaat
      121 ttaaaaatta aaactactta ttctatcaaa atgttagcga ggaggggtca taaattgtat
      181 aaagtagatt taaatagtga tttaggagaa agttttggaa cgtataagat tggtttagat
      241 gaagaagttt taaaatatat ttcatcagcc aatatagctt gtgggtttca tgcaggtgac
      301 cctagtcata tggaaaaaac agtacagcta gctaaaaaaa atggagtgaa aataggcgca
      361 catccaggtt tcctagattt aataggattt ggaagacgtg agatgaaaat aacaaagcag
      421 gaagctaaag attatacaaa atatcaactg ggtgctttaa tggcatttgc aagttcaaat
      481 ggatgtaata tacaacatgt aaaaccacat ggagcattat acaatatggc agctaaagat
      541 aaagagttag ctatggggat ttgtgaagct atatatgaag tcgataaaga tattatactg
      601 ttggggctat ataatagtga aatgataaat tctgctaaag aaattggact taggtttgca
      661 aatgaagtat ttgcagatag agcttatgat aataatggtt ttttggttcc aagaaatgta
      721 gaaggagcag ttatacatga tacaaaacat gcaatagata gggttgttag gatggtaaaa
      781 gaaggaactg tagaaacttt aacaggtgaa gttatacata tcaaagcaga ttctatatgt
      841 gtacatgggg acaatccaaa agcaatagag tttgtcaaag aaataagaaa gcgttttgaa
      901 ttagaaggta tagaagtttg tccattagaa aacatagaag tttgttcgtc agagaacata
      961 gtataagact gataacatga gggggagata aaatatgagt acaaaaaata ttacagataa
     1021 aactaaaaat aaaaaagatg tcggagcact tataggggct gcatttataa tggcaacatc
     1081 agcaattgga ccagggtttt taactcagac tgcacaattt acccaagatt ttggaccgaa
     1141 tttttcattt gtaattttaa ttacaacaat cctttttata ggggctcaag taaatgtatg
     1201 gagagtaatt ggtgtatcag gacttagagg tcaggatata gcaaataaaa taatacctgg
     1261 attaggctat ttagtagctt ttttagtagc tcttggagga ttagctttta atataggaaa
     1321 tgttggtgga gctgcacttg gtatgaatgt aatgtttaat atgaatatga cattaggaac
     1381 tgtattaagt ggattaatag ctatatttgt atttatgtca aaaaattcaa attctctagt
     1441 cgataagata actaaatttt tagcattagg aatgataatt atagttggat atgtagcaat
     1501 atcaaatcat cctcctgttg gagaggcagt atcaagaatg gtaaaaccag aaaatccaaa
     1561 aggactgata ttcccaataa taactttact tggaggaagt gttggaggtt atataacttt
     1621 tgctggagga catagattaa tagacggtgg aataacagga gaagaaaata taaaagaaat
     1681 aactaaatct tctcttttgg gtatattagt tgccacaatg atgagagttc ttttattttt
     1741 agcaatactg gcagtggttt caaaaggtct tcaacttgac ccagaaaatc cagcagcttc
     1801 agcttttaaa tttagtgcag gagcaatagg atataaattc tttggacttg tattatggtc
     1861 tgctgcaatt acatcagtta taggagctgc ttatacatca gtttctttct taaagacact
     1921 aaatcctttt atagataaat atgaaaaata ttttataata gcatttatag caatatcaac
     1981 acttataatg gcatttatag gaaaaccagc tactttatta attttagcag gagctttaaa
     2041 tggattgata cttccaatca cattaggaat tatgcttata gcatctaaaa gaaaagatat
     2101 tgttggagat tacaagcatc ctacatggct tttaatattt ggattaatag tagttttaat
     2161 atctgcatat acaggaataa cttctcttag cagcctaggt gctttatttg cttaatttag
     2221 aagaggtgac atttatggaa actagatact tgatatcagg ggataaggca gtggttgctg
     2281 aatttggaaa tgagatatca gaagatataa acaaaaaagt aatttcattt atgagagcaa
     2341 tagaaatatc gaatcttaaa ggtattgtaa cagaaatggt accaacatat aggtcactta
     2401 tgataagtta taatccatta gaaatagatt ttgatagttt gattgagaat ttaaaaaaaa
     2461 tagaagataa tttggaaagt atagtattac caaaacctaa aatacatgag attccagtat
     2521 gttatgatga ggtttttggt atagatatta aaaacgttgc aagctacaat aatctcactg
     2581 tagatgaagt tataaaaata cacacaagca gagagtactt gatatatatg ctagggttta
     2641 ctcctggatt tccatatcta ggtggtatgg atgaaagaat agctacacct agattagaag
     2701 tgccaagaac taaaatttat ggtgggagtg ttggaatagc aggttcccag actggtgtat
     2761 atcctattga tagtcctggt ggatggcaaa taattggtag gacaccttta aaattatatg
     2821 atgaaaatag agaagaacag attttgctta gagctggaga ctttataaaa tttgttccaa
     2881 taacattaga tgaatttata gaaatcgaaa aaaataactt aagtatttaa atatcgttat
     2941 agtacaggga gtggtatata tgggattcaa agttgaatta ggaggtttcc aaacattaat
     3001 acaagacaga ggtagagttg gctatggtca atatggtgta tctggatgtg gagcaatgga
     3061 tgagtatgca catagagtag gaaatatatt agtaggaaat tctgaagatg aggcttcttt
     3121 agaagtttta atgttaggtc ctacaattac ctttgatgaa tatacgcaaa tagcagtaac
     3181 tgggggagat ttaggggcta aaataaatgg aaaagaaatt caaaactgga ggtcatatca
     3241 aataaatcca ggtgatgtat tatcatttag aggtgtgaaa tcaggtacta gagcctatgt
     3301 gtcaatagct ggtggaatag atgttccact tgcaatggga agtaagtcaa cttatacaag
     3361 agctaaaatt ggtggatttg aaggtagagc acttaaaaaa ggcgattata taaatacttt
     3421 tattcaggaa aaagatttta caataaataa aaaattaagt tcaaaatata ttccaactta
     3481 tagttcagaa atcgtactaa gaattgtaaa aggtcctcaa tttgatgctt tttctaacgg
     3541 tgaagtagag aaatttttat caaataaata caaagttacg aatgaaatag atagaatggg
     3601 ttgtagacta gatggagaga gtataaagca ccttaatgga gcagatatta tatctgaggg
     3661 tatttcttat ggagctatac aagtacctgg tcatggaaaa cctattataa tgttatcaga
     3721 tagacagact agtggtggct atactaagat agggaacgtt ataagtgtag atttgtataa
     3781 gttagcacaa gcaaagccaa atgatgtagt aaaatttgaa cttgtagata tatatgaagc
     3841 acatagacta ttgagagagc aagaagataa gattcaagat atctacaaga gtatgaaaaa
     3901 tataagagtt gtaaaggcaa aagtattaaa tgatatagca gtttaatttt aacaaataca
     3961 atttttatta tagtttataa tataacttac aaatagctat ttactattta ccataattaa
     4021 gatggtttag attagtcaaa tagctatttt tttagtttga gtaaatattt taaatttaaa
     4081 taaatacttc aattttaaaa taaaagcaat atttaaatct attatattat ctacaaaaag
     4141 taggtgtgtc atattgagtt tatttagatt ttattggcca aattggaggt gatataactc
     4201 catatccacg agctattgta tctttacttg gattgatgat tttatgtggt acattagctt
     4261 ttataaatgt tgagtcacct ggatttaatc taactacctt ttcacccata caaatatcaa
     4321 ccacaccact cgttaccacc ataatttctt ctgatgcatg atatatataa tctttggcat
     4381 cctcactttc tggtttaagt tcaaactcta caaataaaat tgaaggtgta aactcagttg
     4441 taggcattgg agatactatc tcgtaaaaca tttcactctt tggaaatttc atcataactc
     4501 tatcttcttt tctaatgact aaatcatcag tattattgtc atccataaac atatataaag
     4561 gagtgtctaa aacttcagcg atttttctca atgaagacaa ggatggttca atagcatctc
     4621 tttctatttg agagatataa cttatagata aatctgtcaa tttggaaaca tcacttaatg
     4681 tttttttctt gctttttctt atatttctca tcttttcacc tagcataaga acctccaaat
     4741 aataaaatta atataaaata ttcgcttaaa acaaagtaat tatagcacaa catagaagta
     4801 gtggcaataa gaatacatag tagattttga caattaaaat ttaattaaat ccaaataaaa
     4861 gtctatgaaa atgaattatt gttaaattaa tacaaagaaa ataataataa aaaaatttaa
     4921 tagaagagta ttaattgaat taagattatc acattatatt ttggaaaaga gtaaattttc
     4981 agtaaattat cttgacatat atattgaagt aataatataa tttaaagtat aaaattttaa
     5041 aaattgaatt ataacttcaa aaaataagga gggtaatatg aagataacag atatcaaatt
     5101 tgaaaagctt agaattaaat taaaaaaacc tgtagtagtt tcatttggag taattgaata
     5161 tggagaaagt ataattttaa aaattgaaac tgatgaaggg tattgtggat ttggcgaagc
     5221 agcgcctctt gcagcagtta caggtgaagt tttggataat gttctttcag tattgcttat
     5281 gtttaaaaaa gagttaatag gcaaagaccc actagatatt gaaaccatac atacaatcat
     5341 ggatggaatt attataggta atacttctgc aaaagcagct attgacatag ctttatatga
     5401 tattaaagga aaaattatga atgcacccct ttataaagtt cttggaggat ttgatagtaa
     5461 agtccaaacg gatattacta taagtataga taagccagaa aaaatggcag tagaagcttt
     5521 agaaagagta aaagaaggtt ttaggatatt gaagcttaaa gcaggtataa atcctgaaga
     5581 cgatattgaa gctgtaaagt taattagaga agccgttgga gagagtataa gaattagaat
     5641 tgatgcaaat caaggatgga acgtaaatag cagtataaat actatcaaaa agttagaaga
     5701 atttaatgtt gatgcaattg aacaagcact tccacattgg gatttagatg gtactgctta
     5761 cataagaaat aaaagtaata caaaaattat gatagatgaa tcattacatt caccaataga
     5821 tgcaattaag gctataaaaa agaatgcagt tgatacattt aatattaagc tgatgaaatc
     5881 aagtggaata tatcctgcta taaaaatcaa caacatagct gaggcaagtg gagtaaattg
     5941 tatgttagga tgtatgctgg aaacgagaat tggtataaca gcagcagcaa atttaatagc
     6001 atctaaaaag aacataacag aagctgacct tgatagtttt atgttttgtg aagaattaga
     6061 aggtatatct ggaggatttg taatggatgg agatattatg aatcttgtca ataaaccagg
     6121 tttaggaata gaagtaaact tatgataaat ttggaggtta aatatgaata cacttttttc
     6181 aaatccaggt agtatacttg ctgtaatgac atcaatgatt gcattgggtt tttacttaca
     6241 aagatacaaa gccattaaaa gcttaggacc agcacttaca ataatcataa tgggaataat
     6301 actttcaaac ttaaaagtag ttccagtaag tactgagtta tatggaacta tttcaacata
     6361 tgctattcca gtttctatga ctataatgtt gatgagtgtt gacttaaaag aaatgacaaa
     6421 attatcaaga gaacctttaa ttgcaatttt tgtagctgta ttaactgtaa gtatcatggc
     6481 atttttattt ggattagtat ttgcagaaaa aatatcagaa ggttggaagg ttgctggaat
     6541 gtttgtaggt acatatactg gtggaagtgc taatcttaca gctattggaa caggtcttaa
     6601 tgtaagtaga caaactcttg ctgcagcaaa tgcagcagac tatgtaattg gagtaccaac
     6661 attgatattt atgtttgctc taccagcaat attaaaaaat tcaaagaagt ttaaaaaact
     6721 ttggccatat catgtagaag aatcagaatt agaggattgt caaaatgaag aatttatgga
     6781 gtcaaaagaa tggagtatta aagatatagc ttggatgttg gctataggtt ttgttgttac
     6841 agaagttgca acaatacttg ctggatattt caattcgagt tttagtagtg cagcaagaat
     6901 acttttagtt acaacaattt caattataat tgctcaatta aagccagtaa agaaactaaa
     6961 gggaaattta gatttaggat tatttgttgc attattcttt ttatgtacaa taggtttttc
     7021 agtagatata aaagaatttt taggttcaac ttttactata acactttact gttttagcat
     7081 aatttttgct tcatttgttt tccatttagg cataactaga cttttaaaga taaagtatca
     7141 atatgtaata ctttctatag taggagcaat tgctgatggt cctacttcag ctttagtagc
     7201 tgcttctgca aagtggaatt cgcttgtaag tgtagcagtc gtaatgggag taattggtgg
     7261 agtattagga aattatgcag gtatatcagt ggcatatgca ataaagatgt ctctaggatt
     7321 atagggggga gtttaattga taaaatttaa attatacata tctggactat acagtggaaa
     7381 tgtaatcttt gatggagatt tattaattga aaagttgaat ccttttacaa ataaaataga
     7441 aagtttaaaa ccaataagca aggaagaaaa tacatactat ttaaatctta caaaaataga
     7501 tttaaagtct ttatttaata attttgatgt atatactaaa agcttagtaa acacagataa
     7561 agacacagtt ataaataact taggagaaac gcattctaaa cttaatgaat atatatgggt
     7621 tcaaagaaac aaaaagttcc cactagatat aattattgta gataataaaa tagtaggttt
     7681 tatctgttta tccagagaaa cttgtactat attaattatg gatggatatg aagagtatac
     7741 tgtattaaaa gaatgggaaa aaactcataa aaatgaagag atatattcta taagatttgg
     7801 tggaaattat atgattgata tgaaagatgg tataaaactt tctacagatg tatatctccc
     7861 tgattttgta gattctacca aaaaagcacc gacaattctt atgagaactc catatggaaa
     7921 agaaaatgat aaagaaatat attataaata tgtacaaaga ggatatgctg ttgtaataca
     7981 agatgttagg ggaagaaatg aatcagaagg taagtgggaa cctatgattc atgagaggga
     8041 agatggagat agtaccataa attggatagt atcacaggaa tggtcaagtg gaatagttgg
     8101 aatgcttgga gcatcatatc ttggttatgt ccaatgggca gctgcatctt caggcaataa
     8161 gcatcttaaa gctttagtta gtatagtaac atcaggaagc ccttttattg atataccaag
     8221 aaaaggagga gcttttgtat ctggtatgct tgcatgggca tttatggtat caagaaataa
     8281 agttgataga agcaaaatgg taagagatga ttgggatgat gttttaaaca tacgaccaat
     8341 agaaaatata cctgttgaag ctcttggata tagaattgaa tttctggaag aatggttaaa
     8401 gagagtagaa aaagatgagt attgggattt gatggattgg catttacaaa aagataaaat
     8461 aaatgtacct gcattggttg tatctgggtg gtatgatgat aatagtatgg gaactacaga
     8521 ggccttagat gttataaagg actatgaaaa aggaaagaga aaggctattc taggtccatg
     8581 gatgcataat tcaaatactt taagagatat aaatggtatt tctttaggaa atagttcatt
     8641 aagatatgat ttggattata attatctatt atggtttgat aaatacttaa aaggtataga
     8701 aaataacata gatacgactg ctccagtaga gtattactct gtaggttcta ataagtggaa
     8761 gacagaagaa aattggccaa taataaataa gatagataaa agtatgtatc taataagtga
     8821 tggaaatgct aacacatctt taggaaatgg tagattagta tttgataatg gtttagaaga
     8881 aaagtatgat agttatatat ataatccaaa agacccatca gtacaactaa ttgatatgtc
     8941 agagaatgaa gttggagttc caaataacta caaagattta gaaaaaagaa gtgatatgtt
     9001 gtgttataca tcagatgctt ttagtgaaga atttactgta acaggtgata taaagttaga
     9061 gttttttgca tcaagttctg ccaaagatac tgactgggtt ataaaaatta tggacgtgga
     9121 tttagatgga aattcaatta aattagctga tggtatactt agtgctagat ttagaaatag
     9181 tttttataag tcagagttta tggaagaagg agagatttat aagtttacga ttataacttc
     9241 aaaaatatca aacacgttta aagttggtca taaaattaga ctagatataa cgtctagtgc
     9301 taaaaacttt attttccaaa atagcaacac gacagaggga tataatagta ttgaatatat
     9361 agaagcaaag aatacgattt atcatggagg taaatatcca tctaaattga tattgccaat
     9421 agagaataag taaatttata tagattaaat agtgaggagg tgtctcaaaa tgaatttttt
     9481 agttcgtgag acactttctt ttatacaaaa tcaaatgtat tttcatcatt tcaacaatga
     9541 aaaagaggct tatctctctg ttttgagata gcctcctata agctaaattg attattttgt
     9601 agctgcaaca acgctcattt caactaacaa atgctctcta gcaagtctag cttcaacaca
     9661 agctctagca ggttcaaatc ctttttcaac ccaagcatcc caaacagagt tcatagcttc
     9721 aaagtctttc atatctctta aataaatagt aacagatagc aaatgttgct tatctgaacc
     9781 atatttgttt aataaatctt ctattttagc taaaacttca gttgtttgtt caattactcc
     9841 tccttcagca tgagtttgtc cacataaata aactgtattg ttatgaacta cagctctact
     9901 cattcttcca gttccttcat atctctttat ttccatattt taaatacctc catagttaaa
     9961 aaatctatta aaaatctaaa taataaatta aaaaaagtct atactatata aaattatatt
    10021 atatattctg ataataatcc ataaaaacat ataacttttt agctataatt attattgtta
    10081 ttaattgaaa agatttatta gttacttgac atagtttaca cttttaatta tagtataaat
    10141 tggaatattt agataattat tatttaatgt ataagctaag tttttaatta atatggtttt
    10201 atacatttat atagaaaaga ggtacgtatg agtagcacaa tcaaatcaaa tcgaataaaa
    10261 ggtataatgt ttataatagc atcagcgctt ggctttgcaa tgatgtctgc attcgtaaag
    10321 ctagctggag atttaccaag ttttcaaaaa gtgttcttta gaaatctggt atcagctata
    10381 atagcttttt ggcttatcat aaaacataaa ggaagtctaa ctggtaaaaa agaaaattta
    10441 aaagtacttt tatataggtc aatatttgga actctaggag ttatatttaa ctactatgca
    10501 attgacaagt tgatgctttc agatgctaat atgctaaata aaatgagtcc cttccttgta
    10561 gtaatatttt gtgcaattgc tctaaaagaa aaaataaatg taaagcagat aggagctatt
    10621 ataattgcat ttataggagc tttattcata ataaaaccaa catttagtgt agaaataatt
    10681 ccatatcttg ggggaattgc aggagctgta tttgcagcaa tggcatatac ttgtttaaga
    10741 gttcttggag atagagaaga ttactataca attgtatttt tcttttcagt tttttcatta
    10801 gttactgtag ggccaattgc ttttgcagta tatgaaccaa tgacccttat gcaacttata
    10861 tatctattac tagggggagt atttgcaagt ctaggtcaat ttggaataac tctagcatat
    10921 aaatatgcac ctgctaagga aatctctata tttgactata gtaatataat cttttcagca
    10981 attttaagta tattcttatt caatgtctat ccagatatgt taagtgttgt tggatattta
    11041 atagtatttt cggcagcatt ttatatgttt ttatacaata agaagttaga taaattagac
    11101 aaagaaaaga agaagtaaac tagataaaag tgtttttata atttgtattg aatatttaag
    11161 tctttgtatt aaaattaaag aaaatttaat taaaaatgtt tggtatatta acttataaca
    11221 gcagttaaaa ttgaaaaatt ataactgctg tttcttttat taaaaatatc caaatgtctg
    11281 taaaaataga ctataataga gtagtataaa taatataaag gggataaatt aaagtatgga
    11341 aaaagtgaaa gttgctgtga ttggagcagg taatagagga acgtatgcat atgctccata
    11401 tatttatgaa aattctgatg tatgtgaaat agttgctgta gcagaaccta aaaaaggaag
    11461 acgagaacta tttacacaaa aatataacct agatagtaaa aatgtatttg aaactttaga
    11521 agactttttt aaacatgaca agatggcaga tgcagtaata atagctacaa atgatgatag
    11581 acattatgat gtagcaaaac tagctcttga aaaaggttat catgtactat tagaaaagcc
    11641 aatgtctaat agtttagatg gtctggtaca tatagatgac ttgtgtgata agtataaaga
    11701 taagattttt atgatttgcc atgtactgag atattctcca ttttataata aattaaaaga
    11761 aattgtagaa agcaaaaaat taggagagct agttagtata caatataatg aaaatatagg
    11821 atattggcat tttgcacata gctttactag agggaattgg agaaatagca atgaaaccag
    11881 ccctttaatt ttagcaaaga gttgtcatga catggatatt ttgttatatt tagtaggaag
    11941 tagatgcaaa aaaatatctg catttggaag cttaaagcat cttaataacc aaaatgctag
    12001 tggagagatg gcacaaaatt gtcttcagtg tttagttgaa aaaaaatgtc catattcagc
    12061 aaagagaatt tatttagaaa aagatagaag tataaataga gctgttcaca taaatccaac
    12121 tgaagagaat ctattaaata tacttaagac aagtccatat ggaagatgtg tttacagatg
    12181 tgataataat gttgtagaca atatggtaaa tatactacag tttgaaaatg gagtgacggc
    12241 tacttttaat ttatgtgcat ttactaaaga aaatggaaga actataaagt taatgtttag
    12301 ccatggtgaa gttggtgggg atttaaataa aaatgagata agaattaaag aatttggaaa
    12361 aaacgaagaa atagttatga atccatcaaa tcaacagaat atggtagagg aatatgatag
    12421 aaatcttata gctgaattta ttaaattagt ttcaaataaa gaactagaaa agggtagagt
    12481 agcagcaaaa gaagctatac aaagtcatgt aatggcattt gcagcagaat attctagggt
    12541 cagtgatgaa gtagtgtata tagaagaatt ttttgatagt gctaagcaaa tgactaagga
    12601 gatagaagaa actatatttt aattaataaa aatttattaa ataaaataaa gatagattaa
    12661 ataatagaaa tacgaaaata tgtgttctat taaggaatct atctttttta ttaaggtttt
    12721 ttcttatccc tatcaaaaaa atccatacat tctgataaaa gataagaaag taaagcgaat
    12781 aagatttctg tacttaaagg aatcgttact gaatccataa aggtatcaat tatgttcata
    12841 gctagaaaag ttagtataaa atcaattata aattcgcaaa gtttatatat tgagtatggt
    12901 aatttgttta catatctgaa tatatctaaa atagtagtgc atagaaaatc aattggagtg
    12961 gttatacaaa aatatattaa aaagaaaatt aatacagctc caagagattt atattcaaat
    13021 cctaagaatg ataagaagcc aattcctata accgtgaaaa tacaaaaagt tataccaaaa
    13081 aaaacagcaa aaaataaaaa tacagaaata aattcgccta aaggaatcct tcttttatta
    13141 cttatcttaa tcaactcctt aatttatgat atgaaattta tataaataat aacattctta
    13201 aaaattcata atagtcctct gtttcaaata gtactgtttg agaatctatt tttttcatat
    13261 ttggataaaa agaggcctta aaagctttat aatgttctct aaattttatt tctatcttaa
    13321 atttatctgg caattttatt aagtgcctag ataaatctcc agataaagct tttttgacac
    13381 ctttttcaat tttttttagt gcaaggtttg gatggattga aattgaacca tttccaattc
    13441 cttttgacac aggaacagtg actatatttg gatttagttt ttttgcactc tcacaaagca
    13501 tttcatctcc acttaaaaat gctacaggaa cattataata tgcagcaata taagcattta
    13561 tagtaaattc agatgctatt tcatcattta ttttaaaata gtcataatca atatccatag
    13621 tatgggaaag tggattacca ttacaagtag ctccagagtg atagccggtg aatatacatg
    13681 catcaaagct ttcatcaatt ccagccatca taactagtgg gtctttggtc catcctctaa
    13741 gtattttgac gttttcaggt aaaatatttg gattaatatt tctaccactg tcatgtgcat
    13801 ctttaacaag tatttcatgt acaccagatt gagtagctcc atcacatgct gctgttatct
    13861 ctttactcat ttgtgttcta tagtgttgcg aaaattcaca ttcaagtttt gtttcatccc
    13921 aatttacaat accacaagta ccttctatat cagcacttaa atacaatttc ataatatata
    13981 cccccttatg tttattattg atgataataa cagataacag aaaatacata atcaataaag
    14041 ggcataaaaa tattatgtct tactattaat tattattaaa tctgtaactg aaaaataact
    14101 aaattggtat taaaaaatat aacactatgt gtcatttatt tatatagttt ttatatctga
    14161 tgtttatata aaattgtaac atataagtgg ttacatttac tagtttaaca tagttaaaat
    14221 agttaggtat tatttttaag ttaaattgat tattataaaa tcgcagaata aaagaaagat
    14281 ttaatttatt aaaatgcatg aaatgttttt tcctcaacat aatgctaaat taaactcaaa
    14341 aagaaataat agaaaaatct tatgtagctt agaaagaatg tgaatgattt aagggaaaaa
    14401 tagaaattta ttatataata ttaggctgta ttatataata cagcctaata ttatatgtat
    14461 atgtgacata aaatcttggt aagtgtaaat aaaataaatg tggaaaacaa gttaatgaaa
    14521 tcaagaaaaa agtaatttaa aattcaattt catcttccca attaaattct cctgattttt
    14581 ctacactctt aggccagtga ctacaccatt taggaatgga cggattttca accctatcaa
    14641 cgcttggagt atatggcttt aagtctataa caggactatt atcattagca tcaatatatg
    14701 gtatttttat tataccattt tcatggtcga tattgataat ttgtacagca gtcaatgcta
    14761 ttggattggg tcttataggt gaccttgttg caaatattcc tataacgtct ggtccacttt
    14821 tataaggttt tggggtttct aaaatacttc ttagttcagg attatcaaac ccatcagccc
    14881 accaaaatac atttaaatga ctgaaatcgt cgagttcatt taatgcaggt acatactttt
    14941 tgtctaattt aataaagacc tcttcgcctt gaatacatat tttacctata ggatttatta
    15001 atatttcttt cataatatta cctccaatta tttaatttat catttgtaat tttagggtat
    15061 aacatgacat catgtgaggg tcaatataaa ataataaata aaaatattaa tagttagaat
    15121 gataatacat tttttacatg gagcatgaca ccagtaataa ggcaatcttc gtcaatatca
    15181 aaagtagagg tatgcaaagg ggaagtaatg tttttttcac gatttgcaca accaaggtgg
    15241 taaaaagctc cttcacagtg tcttagaaaa tacgaaaagt cttcagcccc aagagtgggc
    15301 gagtctttaa ggataatatt ttcttctcca aacaattcta cagcattttg tttgacgtaa
    15361 tctactagat ggtttgaatt gataagagct ggataacctt cctcaatttc aacagaaata
    15421 cttcctccaa atgttttaca agtaaaatcg caaatttctc taattcgttt ttttgaaaac
    15481 tctctagttt caggagtcaa ggttctcaaa gtacccctta taaccacttt atcacaaact
    15541 atattttcct tgattccacc ttcaatcttt cctagagaaa taaccacaga atcagttgga
    15601 tttgtgtttc gactaacgat tgtctgtaga gctgtaataa tatgagcaga tataacaata
    15661 gcatcaatac cttgatgtgg ataagctcca tgacattttg aaccatgtac agttaattga
    15721 agtgtatcag tggaagcatt tagagtgttg tattttaatt caattagatt tttgttgata
    15781 ttggaattta catgaagtcc aaatatatat tttacatttg ggttttcaag acatccatct
    15841 tctatcataa gttgagcccc accaatagtt tcctcggcag gttgaaaaaa gaattttaca
    15901 tttacattta agtaatcctt taatttaaat agtatattac aagtacccag taaaatagca
    15961 gtgtgtgcat catgaccaca tgcatgcatt ttgccaatat taatagattt ataagaataa
    16021 ttaagttctt ctgttatagg taaggcatct atatcagctc ttatagcaac tgtttcaaag
    16081 tttggactca cgttaatgta tgcagttatc cctgtatgat tcttatattc aatataattt
    16141 atacctattt catttaagta tttaataatt tttttcttag tttgaaattc ttctaatcca
    16201 agttctggag tcttatgtaa atctcttctt atatttataa cccaatcatt tatctcatga
    16261 caaagagttt ttatattaag cataaaacac atcctaacta tattaaaaat ttatttttct
    16321 tcattaatta tattatttta tgtaaatcat tattatgtat caagtacaaa taaaatatct
    16381 tataattata ttagagatat ttagtatgga gaagaatata tcattttata agaagaggtg
    16441 tttaaatgtt aggaataatt gcgccatctg ggccattaag aaatacaagt ttggaggaaa
    16501 taaagtttaa tttagaaagt tatggttatg aagttaagtt ttctgaaagt tgtagtttaa
    16561 attataaagg ttatttagct ggtaatgatg atattagagc aagggatata gaagatatgt
    16621 ttttagataa agatgtagat ataattatgt gcttaagagg aggttatgga acaacaagaa
    16681 tattggataa aataaattat gatataataa agcaaaatcc aaaaccattt ataggttttt
    16741 ctgatataac aggtttaaat ttagctttct ataaaaattg tggtctttta ccatatcatg
    16801 gaataatggc agcaaatgta ggtaaatggg ataaatttac ctataaatca ttggtaaatg
    16861 ctttagaatt taaagatgaa ttatatttag aaaatccaaa agaagaaaag atttatactg
    16921 tatgtgaagg taaagcagag ggaataataa tgggaggaaa tttatcctta ataattgcca
    16981 caatgggaac aaaatatgaa atagatgcaa aagataaaat tttgtttatt gaggaaattg
    17041 gagaacctca atataaactg gatagaatgt taacacaact atattcttca ggaaaacttg
    17101 aggaatgtaa tggtataatt tttggagatt ttaaggattg tattgaagaa aatgatttga
    17161 tggaattact aatagaattt gcaaataagg tcaataaacc ttctatatat aatcttcagt
    17221 caggacattg tatcccaatg ataactattc ctttgggcag aatgtgcgaa ttagatgcta
    17281 cagaaaaaat cgttaaatta aaaaaatagt gtgtatataa atataataga aatatggtat
    17341 aatcttatac aagtaaaaat ttagctagga ggatgaaaaa tgtctgatga tagaataata
    17401 gactttaacg aactaaagaa caaagttaaa gattctgatg tagataaatt tgagcaatac
    17461 atatataact tatatttttc tgttatggat ggaaagatgt ctatggctga gttttcaaga
    17521 aaaatattcg attatatgag agataacaac atatctcaag aaaaatttat gaaaatacaa
    17581 aagcaattta tggagagata tggtatggac actgaagaag tagaaaaaca acttagaaac
    17641 tttggaatcg acccttctac ggcaggtttt atgagtaata atacttccag caaagtatct
    17701 acagaagatt tagagtcatt taagaagagt gcaggatttt atgagaaata tggagaaaaa
    17761 atccaaccaa agagttgtat aacaacattt ataaaaaatg acttaaatga tattaatgta
    17821 ataatcgacc aagaaaagat aatgctttgt agtgatagaa aaataaatct tatggattca
    17881 gaactaaatg agtttttatt agaatataag aatatgttta ataaaaaaat aaaggtagta
    17941 atgtgcgaaa ctacgaataa gtatgattat taaaaaatga atttaaatac tgaagtagta
    18001 tcaattaatt gtactactta aaaataaaaa tcgcaattta ttattcctta taaatttgta
    18061 taaggaatat ctaaactgcg attttttata ttgtgaagat tagtaataat agatagtgtt
    18121 ttaaattgta atcttctcac taaaactgtg tttttctatt agaaatttta ttaaaaataa
    18181 atatctgata attgtattat atgggatgta aagtattaga agcttaacga atctctttta
    18241 atacattaac tccattttga atcatactgt tgaaaaatac taagtttaaa atctcagcag
    18301 ggtgaacgtt aggaatatca taagtatcta ctaaattcat aggtacaact atattttttt
    18361 caatattatg ttgattaaaa tatgatttta gagttattgc aaattgatat atacatatat
    18421 ctgtgcaatc gccaactata attatattat ctatattatc aaaatccaaa ttttctaatg
    18481 caaaaaaacc attagtagaa tttttaggaa gaatttgtag gttttctatc gacttaagtt
    18541 catcaaccaa ttcactttct acatcatttt ccaaacagtg aacaggataa ctcaaaagtt
    18601 ctattgattt aggagtatga gaatcagtaa atgctattac tctatttaat ttgtttgata
    18661 ttttttttgt aaacatttca attggcttta ttaaagactc tactctagga gagtataatg
    18721 caccttgtct tgcaaagcca ttattaacat caattataaa taaagcagtt ttactcaaat
    18781 cataattttc tattttttct attggtagat tatctaaatt actctttaaa gcttcaagtt
    18841 catttaataa gttatccata gttatacctc caagataaat ttaataaaat tgtacatatt
    18901 aattatataa aagtcaagga gtacaaacta ttgtataatt agctgtattg ttttgagtgt
    18961 aaaagatttg aagcaatatc tggatagtta gtaaaaatac catctacatt gagttttaga
    19021 cagtgtagta tatcatcctc ttcatttaca gtatatacat tgacaccaag tagatttttg
    19081 tgacaaagtt caattagaga ttcatttact aaaactagag gtggatgaag ataactatta
    19141 cccagaaatt tagcaaaatt gactacattg tttataggta aataacacaa taatcctgtt
    19201 tttacactag ggtctaattt atgaaagttt tttaagctat tgctgttgaa actagatata
    19261 agaactctat ttttcatatt atactttaaa ataagctcta aaacgccttt ttctatattg
    19321 ggatagttta ttttgtcagt ttttagttcg atatttaaag taaggtctgt aggtgagatt
    19381 agaattaata cttcttctaa agttggaatt ttacattctt tattgctttg atatccttca
    19441 aaagatgagt ttagagtttt taattttctt aaagtaaagt cttttacaaa accatgtcca
    19501 ttaaaagttc tatcaacctt ttcatcatga ataactacaa tttttccatc cttagttctg
    19561 tggacatcaa gttctattcc atatatatcc atatccaaac attttttaaa agctagaata
    19621 gtattttctg gatactttcc actaaacccc ctatgagcat aaatattcat ttatatggtt
    19681 ccctcctata agtataactt atcttataaa aatttaatta atataatata tcatattcca
    19741 aaaggtattg ttatatatta gttaaataac tatttgtaat ttatatatta tataaatgta
    19801 gaaa
//
LOCUS       DAYAOX010000056        19601 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.056, whole genome shotgun sequence.
ACCESSION   DAYAOX010000056 DAYAOX010000000
VERSION     DAYAOX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.056"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            220..7320
                     /gene="tcdB"
                     /locus_tag="ACGI57_001599"
                     /gene_synonym="toxB"
     CDS             220..7320
                     /gene="tcdB"
                     /locus_tag="ACGI57_001599"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Toxigenic strains produce this toxin (TcdB) or
                     TcdA. TcdB-producing strains are tumorigenic in mice;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HFG9716679.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            7442..7942
                     /gene="tcdE"
                     /locus_tag="ACGI57_001600"
     CDS             7442..7942
                     /gene="tcdE"
                     /locus_tag="ACGI57_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HFG9716680.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8021..8182
                     /locus_tag="ACGI57_001601"
     CDS             8021..8182
                     /locus_tag="ACGI57_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716681.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            8247..8453
                     /locus_tag="ACGI57_001602"
     CDS             8247..8453
                     /locus_tag="ACGI57_001602"
                     /inference="COORDINATES: protein motif:HMM:NF022413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BhlA/UviB family holin-like peptide"
                     /protein_id="HFG9716682.1"
                     /translation="MKLFKNTKIRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKT
                     LKKYKICLQITIRQSPYLIEESIN"
     gene            8670..16802
                     /gene="tcdA"
                     /locus_tag="ACGI57_001603"
                     /gene_synonym="toxA"
     CDS             8670..16802
                     /gene="tcdA"
                     /locus_tag="ACGI57_001603"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HFG9716683.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17137..17835)
                     /gene="tcdC"
                     /locus_tag="ACGI57_001604"
     CDS             complement(17137..17835)
                     /gene="tcdC"
                     /locus_tag="ACGI57_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HFG9716684.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            18261..18506
                     /gene="cdd1"
                     /locus_tag="ACGI57_001605"
     CDS             18261..18506
                     /gene="cdd1"
                     /locus_tag="ACGI57_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Cdd1"
                     /protein_id="HFG9716685.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(18724..18993)
                     /locus_tag="ACGI57_001606"
     CDS             complement(18724..18993)
                     /locus_tag="ACGI57_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFG9716686.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT         7742 a         2239 c         2987 g         6633 t
ORIGIN      
        1 attttatata gaacaatgtt tacatattta tttcagacaa cgtctttatt caatcgaaga
       61 gcaaattaat caactgagtg tcttaaattt aaaatgttag gaagtgaatg tatatgaaaa
      121 cctaagtaga tattagtata ttttataaat agaaaggagg atatataaaa gagttttagc
      181 atttagatgt aaaaatatta tagtaaagga gaaaatttta tgagtttagt taatagaaaa
      241 cagttagaaa aaatggcaaa tgtaagattt cgtactcaag aagatgaata tgttgcaata
      301 ttggatgctt tagaagaata tcataatatg tcagagaata ctgtagtcga aaaatattta
      361 aaattaaaag atataaatag tttaacagat atttatatag atacatataa aaaatctggt
      421 agaaataaag ccttaaaaaa atttaaggaa tatctagtta cagaagtatt agagctaaag
      481 aataataatt taactccagt tgagaaaaat ttacattttg tttggattgg aggtcaaata
      541 aatgacactg ctattaatta tataaatcaa tggaaagatg taaatagtga ttataatgtt
      601 aatgtttttt atgatagtaa tgcatttttg ataaacacat tgaaaaaaac tgtagtagaa
      661 tcagcaataa atgatacact tgaatcattt agagaaaact taaatgaccc tagatttgac
      721 tataataaat tcttcagaaa acgtatggaa ataatttatg ataaacagaa aaatttcata
      781 aactactata aagctcaaag agaagaaaat cctgaactta taattgatga tattgtaaag
      841 acatatcttt caaatgagta ttcaaaggag atagatgaac ttaataccta tattgaagaa
      901 tccttaaata aaattacaca gaatagtgga aatgatgtta gaaactttga agaatttaaa
      961 aatggagagt cattcaactt atatgaacaa gagttggtag aaaggtggaa tttagctgct
     1021 gcttctgaca tattaagaat atctgcatta aaagaaattg gtggtatgta tttagatgtt
     1081 gatatgttac caggaataca accagactta tttgagtcta tagagaaacc tagttcagta
     1141 acagtggatt tttgggaaat gacaaagtta gaagctataa tgaaatacaa agaatatata
     1201 ccagaatata cctcagaaca ttttgacatg ttagacgaag aagttcaaag tagttttgaa
     1261 tctgttctag cttctaagtc agataaatca gaaatattct catcacttgg tgatatggag
     1321 gcatcaccac tagaagttaa aattgcattt aatagtaagg gtattataaa tcaagggcta
     1381 atttctgtga aagactcata ttgtagcaat ttaatagtaa aacaaatcga gaatagatat
     1441 aaaatattga ataatagttt aaatccagct attagcgagg ataatgattt taatactaca
     1501 acgaatacct ttattgatag tataatggct gaagctaatg cagataatgg tagatttatg
     1561 atggaactag gaaagtattt aagagttggt ttcttcccag atgttaaaac tactattaac
     1621 ttaagtggcc ctgaagcata tgcggcagct tatcaagatt tattaatgtt taaagaaggc
     1681 agtatgaata tccatttgat agaagctgat ttaagaaact ttgaaatctc taaaactaat
     1741 atttctcaat caactgaaca agaaatggct agcttatggt catttgacga tgcaagagct
     1801 aaagctcaat ttgaagaata taaaaggaat tattttgaag gttctcttgg tgaagatgat
     1861 aatcttgatt tttctcaaaa tatagtagtt gacaaggagt atcttttaga aaaaatatct
     1921 tcattagcaa gaagttcaga gagaggatat atacactata ttgttcagtt acaaggagat
     1981 aaaattagtt atgaagcagc atgtaactta tttgcaaaga ctccttatga tagtgtacta
     2041 tttcagaaaa atatagaaga ttcagaaatt gcatattatt ataatcctgg agatggtgaa
     2101 atacaagaaa tagacaagta taaaattcca agtataattt ctgatagacc taagattaaa
     2161 ttaacattta ttggtcatgg taaagatgaa tttaatactg atatatttgc aggttttgat
     2221 gtagattcat tatccacaga aatagaagca gcaatagatt tagctaaaga ggatatttct
     2281 cctaagtcaa tagaaataaa tttattagga tgtaatatgt ttagctactc tatcaacgta
     2341 gaggagactt atcctggaaa attattactt aaagttaaag ataaaatatc agaattaatg
     2401 ccatctataa gtcaagactc tattatagta agtgcaaatc aatatgaagt tagaataaat
     2461 agtgaaggaa gaagagaatt attggatcat tctggtgaat ggataaataa agaagaaagt
     2521 attataaagg atatttcatc aaaagaatat atatcattta atcctaaaga aaataaaatt
     2581 acagtaaaat ctaaaaattt acctgagcta tctacattat tacaagaaat tagaaataat
     2641 tctaattcaa gtgatattga actagaagaa aaagtaatgt taacagaatg tgagataaat
     2701 gttatttcaa atatagatac gcaaattgtt gaggaaagga ttgaagaagc taagaattta
     2761 acttctgact ctattaatta tataaaagat gaatttaaac taatagaatc tatttctgat
     2821 gcactatgtg acttaaaaca acagaatgaa ttagaagatt ctcattttat atcttttgag
     2881 gacatatcag agactgatga gggatttagt ataagattta ttaataaaga aactggagaa
     2941 tctatatttg tagaaactga aaaaacaata ttctctgaat atgctaatca tataactgaa
     3001 gagatttcta agataaaagg tactatattt gatactgtaa atggtaagtt agtaaaaaaa
     3061 gtaaatttag atactacaca cgaagtaaat actttaaatg ctgcattttt tatacaatca
     3121 ttaatagaat ataatagttc taaagaatct cttagtaatt taagtgtagc aatgaaagtc
     3181 caagtttacg ctcaattatt tagtactggt ttaaatacta ttacagatgc agccaaagtt
     3241 gttgaattag tatcaactgc attagatgaa actatagact tacttcctac attatctgaa
     3301 ggattaccta taattgcaac tattatagat ggtgtaagtt taggtgcagc aatcaaagag
     3361 ctaagtgaaa cgagtgaccc attattaaga caagaaatag aagctaagat aggtataatg
     3421 gcagtaaatt taacaacagc tacaactgca atcattactt catctttggg ggtagctagt
     3481 ggatttagta tacttttagt tcctttagca ggaatttcag caggtatacc aagcttagta
     3541 aacaatgaac ttgtacttcg agataaggca acaaaggttg tagattattt taaacatgtt
     3601 tcattagttg aaactgaagg agtatttact ttattagatg ataaaataat gatgccacaa
     3661 gatgatttag tgatatcaga aatagatttt aataataatt caatagtttt aggtaaatgt
     3721 gaaatctgga gaatggaagg tggttcaggt catactgtaa ctgatgatat agatcacttc
     3781 ttttcagcac catcaataac atatagagag ccacacttat ctatatatga cgtattggaa
     3841 gtacaaaaag aagaacttga tttgtcaaaa gatttaatgg tattacctaa tgctccaaat
     3901 agagtatttg cttgggaaac aggatggaca ccaggtttaa gaagcttaga aaatgatggc
     3961 acaaaactgt tagaccgtat aagagataac tatgaaggtg agttttattg gagatatttt
     4021 gcttttatag ctgatgcttt aataacaaca ttaaaaccaa gatatgaaga tactaatata
     4081 agaataaatt tagatagtaa tactagaagt tttatagttc caataataac tacagaatat
     4141 ataagagaaa aattatcata ttctttctat ggttcaggag gaacttatgc attgtctctt
     4201 tctcaatata atatgggtat aaatatagaa ttaagtgaaa gtgatgtttg gattatagat
     4261 gttgataatg ttgtgagaga tgtaactata gaatctgata aaattaaaaa aggtgattta
     4321 atagaaggta ttttatctac actaagtatt gaagagaata aaattatctt aaatagccat
     4381 gagattaatt tttctggtga ggtaaatgga agtaatggat ttgtttcttt aacattttca
     4441 attttagaag gaataaatgc aattatagaa gttgatttat tatctaaatc atataaatta
     4501 cttatttctg gcgaattaaa aatattgatg ttaaattcaa atcatattca acagaaaata
     4561 gattatatag gattcaatag cgaattacag aaaaatatac catatagctt tgtagatagt
     4621 gaaggaaaag agaatggttt tattaatggt tcaacaaaag aaggtttatt tgtatctgaa
     4681 ttacctgatg tagttcttat aagtaaggtt tatatggatg atagtaagcc ttcatttgga
     4741 tattatagta ataatttgaa agatgtcaaa gttataacta aagataatgt taatatatta
     4801 acaggttatt atcttaagga tgatataaaa atctctcttt ctttgactct acaagatgaa
     4861 aaaactataa agttaaatag tgtgcattta gatgaaagtg gagtagctga gattttgaag
     4921 ttcatgaata gaaaaggtaa tacaaatact tcagattctt taatgagctt tttagaaagt
     4981 atgaatataa aaagtatttt cgttaatttc ttacaatcta atattaagtt tatattagat
     5041 gctaatttta taataagtgg tactacttct attggccaat ttgagtttat ttgtgatgaa
     5101 aatgataata tacaaccata tttcattaag tttaatacac tagaaactaa ttatacttta
     5161 tatgtaggaa atagacaaaa tatgatagtg gaaccaaatt atgatttaga tgattctgga
     5221 gatatatctt caactgttat caatttctct caaaagtatc tttatggaat agacagttgt
     5281 gttaataaag ttgtaatttc accaaatatt tatacagatg aaataaatat aacgcctgta
     5341 tatgaaacaa ataatactta tccagaagtt attgtattag atgcaaatta tataaatgaa
     5401 aaaataaatg ttaatatcaa tgatctatct atacgatatg tatggagtaa tgatggtaat
     5461 gattttattc ttatgtcaac tagtgaagaa aataaggtgt cacaagttaa aataagattc
     5521 gttaatgttt ttaaagataa gactttggca aataagctat cttttaactt tagtgataaa
     5581 caagatgtac ctgtaagtga aataatctta tcatttacac cttcatatta tgaggatgga
     5641 ttgattggct atgatttggg tctagtttct ttatataatg agaaatttta tattaataac
     5701 tttggaatga tggtatctgg attaatatat attaatgatt cattatatta ttttaaacca
     5761 ccagtaaata atttgataac tggatttgtg actgtaggcg atgataaata ctactttaat
     5821 ccaattaatg gtggagctgc ttcaattgga gagacaataa ttgatgacaa aaattattat
     5881 ttcaaccaaa gtggagtgtt acaaacaggt gtatttagta cagaagatgg atttaaatat
     5941 tttgccccag ctaatacact tgatgaaaac ctagaaggag aagcaattga ttttactgga
     6001 aaattaatta ttgacgaaaa tatttattat tttgatgata attatagagg agctgtagaa
     6061 tggaaagaat tagatggtga aatgcactat tttagcccag aaacaggtaa agcttttaaa
     6121 ggtctaaatc aaataggtga ttataaatac tatttcaatt ctgatggagt tatgcaaaaa
     6181 ggatttgtta gtataaatga taataaacac tattttgatg attctggtgt tatgaaagta
     6241 ggttacactg aaatagatgg caagcatttc tactttgctg aaaacggaga aatgcaaata
     6301 ggagtattta atacagaaga tggatttaaa tattttgctc atcataatga agatttagga
     6361 aatgaagaag gtgaagaaat ctcatattct ggtatattaa atttcaataa taaaatttac
     6421 tattttgatg attcatttac agctgtagtt ggatggaaag atttagagga tggttcaaag
     6481 tattattttg atgaagatac agcagaagca tatataggtt tgtcattaat aaatgatggt
     6541 caatattatt ttaatgatga tggaattatg caagttggat ttgtcactat aaatgataaa
     6601 gtcttctact tctctgactc tggaattata gaatctggag tacaaaacat agatgacaat
     6661 tatttctata tagatgataa tggtatagtt caaattggtg tatttgatac ttcagatgga
     6721 tataaatatt ttgcacctgc taatactgta aatgataata tttacggaca agcagttgaa
     6781 tatagtggtt tagttagagt tggggaagat gtatattatt ttggagaaac atatacaatt
     6841 gagactggat ggatatatga tatggaaaat gaaagtgata aatattattt caatccagaa
     6901 actaaaaaag catgcaaagg tattaattta attgatgata taaaatatta ttttgatgag
     6961 aagggcataa tgagaacggg tcttatatca tttgaaaata ataattatta ctttaatgag
     7021 aatggtgaaa tgcaatttgg ttatataaat atagaagata agatgttcta ttttggtgaa
     7081 gatggtgtca tgcagattgg agtatttaat acaccagatg gatttaaata ctttgcacat
     7141 caaaatactt tggatgagaa ttttgaggga gaatcaataa actatactgg ttggttagat
     7201 ttagatgaaa agagatatta ttttacagat gaatatattg cagcaactgg ttcagttatt
     7261 attgatggtg aggagtatta ttttgatcct gatacagctc aattagtgat tagtgaatag
     7321 ataaaaatat gttaaatata tcctcttata cttaaatata taaaaataaa caaaatgata
     7381 cactacataa agtgttctat ctaatatgaa gatttaccaa taaaaaggtg gactatgatg
     7441 aatgcacagt agttcacctt tttatatttc taatggtaac aaaatatttt tttatataaa
     7501 cctaggaggc gttatgaata tgacaatatc ttttttatca gagcatatat ttataaagtt
     7561 agtaatttta actatatcat ttgatacatt attaggatgt ttaagtgcaa taaaaagtcg
     7621 taaatttaat tctagttttg gaatagatgg aggaatcaga aaagtagcaa tgatagcatg
     7681 tatatttttt ttatcagtag ttgacattct tacaaagttt aactttttat ttatgttacc
     7741 acaagattgt atcaattttt taagactaaa acatcttgga atatctgaat ttttctctat
     7801 tttatttatt ttatatgaaa gtgtaagtat attaaaaaat atgtgcttat gtggattacc
     7861 agtacctaag agattaaagg aaaaaatagc aattttacta gatgcaatga cagatgaaat
     7921 gaatgctaag gatgaaaagt aagtaatggt agatataata aagatattaa caaataaaaa
     7981 gtgttatcca aataagaata gctgaaagtt atcataattc atgaaactaa taatgaaaac
     8041 gagggagcag atgccaagag acacacaagt attaaataca tataatttcg aagcaagtgt
     8101 tcattactat atagatgaca aggtagtata tcaaacattg gttcacaaag atggtgcatg
     8161 gtcagttggt aaaatctatt aagctacatt agttacagat atcacaaact ataatagtta
     8221 aacatagaaa tatgtgtaaa ttgtgattga aattattcaa aaacacaaaa atacgtgatg
     8281 aaggacaaaa tgatatagaa aataagtatc aaaccttaat aaatgattta attgatagtt
     8341 taaaagttat aggaaaaata tataaagaaa taaaaacatt aaaaaaatat aagatatgtt
     8401 tacaaattac tatcagacaa tctccttatc taatagaaga gtcaattaac taattgagta
     8461 tctttaaatt gaaatgttag gaagtgattt aaatatgaaa acttaaatta taaaaaatca
     8521 atattaattt atttttaaaa aatagaaagg agtgtataag atttattttc aaagtttaaa
     8581 aacaagaaaa tcaatttaaa tttcagaagg aataaatgtg gttatagaag tggatttatt
     8641 atcaaaaata ataatactag gaggttttta tgtctttaat atctaaagaa gagttaataa
     8701 aactcgcata tagcattaga ccaagagaaa atgagtataa aactatacta actaatttag
     8761 acgaatataa taagttaact acaaacaata atgaaaataa atatttacaa ttaaaaaaac
     8821 taaatgaatc aattgatgtt tttatgaata aatataaaac ttcaagcaga aatagagcac
     8881 tctctaatct aaaaaaagat atattaaaag aagtaattct tattaaaaat tccaatacaa
     8941 gccctgtaga aaaaaattta cattttgtat ggataggtgg agaagtcagt gatattgctc
     9001 ttgaatacat aaaacaatgg gctgatatta atgcagaata taatattaaa ctgtggtatg
     9061 atagtgaagc attcttagta aatacactaa aaaaggctat agttgaatct tctaccactg
     9121 aagcattaca gctactagag gaagagattc aaaatcctca atttgataat atgaaatttt
     9181 acaaaaaaag gatggaattt atatatgata gacaaaaaag gtttataaat tattataaat
     9241 ctcaaatcaa taaacctaca gtacctacaa tagatgatat tataaagtct catctagtat
     9301 ctgaatataa tagagatgaa actgtattag aatcatatag aacaaattct ttgagaaaaa
     9361 taaatagtaa tcatgggata gatatcaggg ctaatagttt gtttacagaa caagagttat
     9421 taaatattta tagtcaggag ttgttaaatc gtggaaattt agctgcagca tctgacatag
     9481 taagattatt agccctaaaa aattttggcg gagtatattt agatgttgat atgcttccag
     9541 gtattcactc tgatttattt aaaacaatat ctagacctag ctctattgga ctagaccgtt
     9601 gggaaatgat aaaattagag gctattatga agtataaaaa atatataaat aattatacat
     9661 cagaaaactt tgataaactt gatcaacaat taaaagataa ttttaaactc attatagaaa
     9721 gtaaaagtga aaaatctgag atattttcta aattagaaaa tttaaatgta tctgatcttg
     9781 aaattaaaat agctttcgct ttaggcagtg ttataaatca agccttgata tcaaaacaag
     9841 gttcatatct tactaaccta gtaatagaac aagtaaaaaa tagatatcaa tttttaaacc
     9901 aacaccttaa cccagccata gagtctgata ataacttcac agatactact aaaatttttc
     9961 atgattcatt atttaattca gctaccgcag aaaactctat gtttttaaca aaaatagcac
    10021 catacttaca agtaggtttt atgccagaag ctcgctccac aataagttta agtggtccag
    10081 gagcttatgc gtcagcttac tatgatttca taaatttaca agaaaatact atagaaaaaa
    10141 ctttaaaagc atcagattta atagaattta aattcccaga aaataatcta tctcaattga
    10201 cagaacaaga aataaatagt ctatggagct ttgatcaagc aagtgcaaaa tatcaatttg
    10261 agaaatatgt aagagattat actggtggat ctctttctga agacaatggg gtagacttta
    10321 ataaaaatac tgccctcgac aaaaactatt tattaaataa taaaattcca tcaaacaatg
    10381 tagaagaagc tggaagtaaa aattatgttc attatatcat acagttacaa ggagatgata
    10441 taagttatga agcaacatgc aatttatttt ctaaaaatcc taaaaatagt attattatac
    10501 aacgaaatat gaatgaaagt gcaaaaagct actttttaag tgatgatgga gaatctattt
    10561 tagaattaaa taaatatagg atacctgaaa gattaaaaaa taaggaaaaa gtaaaagtaa
    10621 cctttattgg acatggtaaa gatgaattca acacaagcga atttgctaga ttaagtgtag
    10681 attcactttc caatgagata agttcatttt tagataccat aaaattagat atatcaccta
    10741 aaaatgtaga agtaaactta cttggatgta atatgtttag ttatgatttt aatgttgaag
    10801 aaacttatcc tgggaagttg ctattaagta ttatggacaa aattacttcc actttacctg
    10861 atgtaaataa aaattctatt actataggag caaatcaata tgaagtaaga attaatagtg
    10921 agggaagaaa agaacttctg gctcactcag gtaaatggat aaataaagaa gaagctatta
    10981 tgagcgattt atctagtaaa gaatacattt tttttgattc tatagataat aagctaaaag
    11041 caaagtccaa gaatattcca ggattagcat caatatcaga agatataaaa acattattac
    11101 ttgatgcaag tgttagtcct gatacaaaat ttattttaaa taatcttaag cttaatattg
    11161 aatcttctat tggtgattac atttattatg aaaaattaga gcctgttaaa aatataattc
    11221 acaattctat agatgattta atagatgagt tcaatctact tgaaaatgta tctgatgaat
    11281 tatatgaatt aaaaaaatta aataatctag atgagaagta tttaatatct tttgaagata
    11341 tctcaaaaaa taattcaact tactctgtaa gatttattaa caaaagtaat ggtgagtcag
    11401 tttatgtaga aacagaaaaa gaaatttttt caaaatatag cgaacatatt acaaaagaaa
    11461 taagtactat aaagaatagt ataattacag atgttaatgg taatttattg gataatatac
    11521 agttagatca tacttctcaa gttaatacat taaacgcagc attctttatt caatcattaa
    11581 tagattatag tagcaataaa gatgtactga atgatttaag tacctcagtt aaggttcaac
    11641 tttatgctca actatttagt acaggtttaa atactatata tgactctatc caattagtaa
    11701 atttaatatc aaatgcagta aatgatacta taaatgtact acctacaata acagagggga
    11761 tacctattgt atctactata ttagacggaa taaacttagg tgcagcaatt aaggaattac
    11821 tagacgaaca tgacccatta ctaaaaaaag aattagaagc taaggtgggt gttttagcaa
    11881 taaatatgtc attatctata gctgcaactg tagcttcaat tgttggaata ggtgctgaag
    11941 ttactatttt cttattacct atagctggta tatctgcagg aataccttca ttagttaata
    12001 atgaattaat attgcatgat aaggcaactt cagtggtaaa ctattttaat catttgtctg
    12061 aatctaaaaa atatggccct cttaaaacag aagatgataa aattttagtt cctattgatg
    12121 atttagtaat atcagaaata gattttaata ataattcgat aaaactagga acatgtaata
    12181 tattagcaat ggagggggga tcaggacaca cagtgactgg taatatagat cactttttct
    12241 catctccatc tataagttct catattcctt cattatcaat ttattctgca ataggtatag
    12301 aaacagaaaa tctagatttt tcaaaaaaaa taatgatgtt acctaatgct ccttcaagag
    12361 tgttttggtg ggaaactgga gcagttccag gtttaagatc attggaaaat gacggaacta
    12421 gattacttga ttcaataaga gatttatacc caggtaaatt ttactggaga ttctatgctt
    12481 ttttcgatta tgcaataact acattaaaac cagtttatga agacactaat attaaaatta
    12541 aactagataa agatactaga aacttcataa tgccaactat aactactaac gaaattagaa
    12601 acaaattatc ttattcattt gatggagcag gaggaactta ctctttatta ttatcttcat
    12661 atccaatatc aacgaatata aatttatcta aagatgattt atggatattt aatattgata
    12721 atgaagtaag agaaatatct atagaaaatg gtactattaa aaaaggaaag ttaataaaag
    12781 atgttttaag taaaattgat ataaataaaa ataaacttat tataggcaat caaacaatag
    12841 atttttcagg cgatatagat aataaagata gatatatatt cttgacttgt gagttagatg
    12901 ataaaattag tttaataata gaaataaatc ttgttgcaaa atcttatagt ttgttattgt
    12961 ctggggataa aaattatttg atatccaatt tatctaatac tattgagaaa atcaatactt
    13021 taggcctaga tagtaaaaat atagcgtaca attacactga tgaatctaat aataaatatt
    13081 ttggagctat atctaaaaca agtcaaaaaa gcataataca ttataaaaaa gacagtaaaa
    13141 atatattaga attttataat gacagtacat tagaatttaa cagtaaagat tttattgctg
    13201 aagatataaa tgtatttatg aaagatgata ttaatactat aacaggaaaa tactatgttg
    13261 ataataatac tgataaaagt atagatttct ctatttcttt agttagtaaa aatcaagtaa
    13321 aagtaaatgg attatattta aatgaatccg tatactcatc ttaccttgat tttgtgaaaa
    13381 attcagatgg acaccataat acttctaatt ttatgaattt atttttggac aatataagtt
    13441 tctggaaatt gtttgggttt gaaaatataa attttgtaat cgataaatac tttacccttg
    13501 ttggtaaaac taatcttgga tatgtagaat ttatttgtga caataataaa aatatagata
    13561 tatattttgg tgaatggaaa acatcgtcat ctaaaagcac tatatttagc ggaaatggta
    13621 gaaatgttgt agtagagcct atatataatc ctgatacggg tgaagatata tctacttcac
    13681 tagatttttc ctatgaacct ctctatggaa tagatagata tatcaataaa gtattgatag
    13741 cacctgattt atatacaagt ttaataaata ttaataccaa ttattattca aatgagtact
    13801 accctgagat tatagttctt aacccaaata cattccacaa aaaagtaaat ataaatttag
    13861 atagttcttc ttttgagtat aaatggtcta cagaaggaag tgactttatt ttagttagat
    13921 acttagaaga aagtaataaa aaaatattac aaaaaataag aatcaaaggt atcttatcta
    13981 atactcaatc atttaataaa atgagtatag attttaaaga tattaaaaaa ctatcattag
    14041 gatatataat gagtaatttt aaatcattta attctgaaaa tgaattagat agagatcatt
    14101 taggatttaa aataatagat aataaaactt attactatga tgaagatagt aaattagtta
    14161 aaggattaat caatataaat aattcattat tctattttga tcctatagaa tttaacttag
    14221 taactggatg gcaaactatc aatggtaaaa aatattattt tgatataaat actggagcag
    14281 ctttaactag ttataaaatt attaatggta aacactttta ttttaataat gatggtgtga
    14341 tgcagttggg agtatttaaa ggacctgatg gatttgaata ttttgcacct gccaatactc
    14401 aaaataataa catagaaggt caggctatag tttatcaaag taaattctta actttgaatg
    14461 gcaaaaaata ttattttgat aatgactcaa aagcagtcac tggatggaga attattaaca
    14521 atgagaaata ttactttaat cctaataatg ctattgctgc agtcggattg caagtaattg
    14581 acaataataa gtattatttc aatcctgaca ctgctatcat ctcaaaaggt tggcagactg
    14641 ttaatggtag tagatactac tttgatactg ataccgctat tgcctttaat ggttataaaa
    14701 ctattgatgg taaacacttt tattttgata gtgattgtgt agtgaaaata ggtgtgttta
    14761 gtacctctaa tggatttgaa tattttgcac ctgctaatac ttataataat aacatagaag
    14821 gtcaggctat agtttatcaa agtaaattct taactttgaa tggtaaaaaa tattactttg
    14881 ataataactc aaaagcagtt accggatggc aaactattga tagtaaaaaa tattacttta
    14941 atactaacac tgctgaagca gctactggat ggcaaactat tgatggtaaa aaatattact
    15001 ttaatactaa cactgctgaa gcagctactg gatggcaaac tattgatggt aaaaaatatt
    15061 actttaatac taacactgct atagcttcaa ctggttatac aattattaat ggtaaacatt
    15121 tttattttaa tactgatggt attatgcaga taggagtgtt taaaggacct aatggatttg
    15181 aatattttgc acctgctaat acggatgcta acaacataga aggtcaagct atactttacc
    15241 aaaatgaatt cttaactttg aatggtaaaa aatattactt tggtagtgac tcaaaagcag
    15301 ttactggatg gagaattatt aacaataaga aatattactt taatcctaat aatgctattg
    15361 ctgcaattca tctatgcact ataaataatg acaagtatta ctttagttat gatggaattc
    15421 ttcaaaatgg atatattact attgaaagaa ataatttcta ttttgatgct aataatgaat
    15481 ctaaaatggt aacaggagta tttaaaggac ctaatggatt tgagtatttt gcacctgcta
    15541 atactcacaa taataacata gaaggtcagg ctatagttta ccagaacaaa ttcttaactt
    15601 tgaatggcaa aaaatattat tttgataatg actcaaaagc agttactgga tggcaaacca
    15661 ttgatggtaa aaaatattac tttaatctta acactgctga agcagctact ggatggcaaa
    15721 ctattgatgg taaaaaatat tactttaatc ttaacactgc tgaagcagct actggatggc
    15781 aaactattga tggtaaaaaa tattacttta atactaacac tttcatagcc tcaactggtt
    15841 atacaagtat taatggtaaa catttttatt ttaatactga tggtattatg cagataggag
    15901 tgtttaaagg acctaatgga tttgaatact ttgcacctgc taatactcat aataataaca
    15961 tagaaggtca agctatactt taccaaaata aattcttaac tttgaatggt aaaaaatatt
    16021 actttggtag tgactcaaaa gcagttaccg gattgcgaac tattgatggt aaaaaatatt
    16081 actttaatac taacactgct gttgcagtta ctggatggca aactattaat ggtaaaaaat
    16141 actactttaa tactaacact tctatagctt caactggtta tacaattatt agtggtaaac
    16201 atttttattt taatactgat ggtattatgc agataggagt gtttaaagga cctgatggat
    16261 ttgaatactt tgcacctgct aatacagatg ctaacaatat agaaggtcaa gctatacgtt
    16321 atcaaaatag attcctatat ttacatgaca atatatatta ttttggtaat aattcaaaag
    16381 cggctactgg ttgggtaact attgatggta atagatatta cttcgagcct aatacagcta
    16441 tgggtgcgaa tggttataaa actattgata ataaaaattt ttactttaga aatggtttac
    16501 ctcagatagg agtgtttaaa gggtctaatg gatttgaata ctttgcacct gctaatacgg
    16561 atgctaacaa tatagaaggt caagctatac gttatcaaaa tagattccta catttacttg
    16621 gaaaaatata ttactttggt aataattcaa aagcagttac tggatggcaa actattaatg
    16681 gtaaagtata ttactttatg cctgatactg ctatggctgc agctggtgga cttttcgaga
    16741 ttgatggtgt tatatatttc tttggtgttg atggagtaaa agcccctggg atatatggct
    16801 aaaatatatg tttgataaaa aattattcct gtgctactaa gaaattattt ttatataata
    16861 aatattgaga tttaattaaa agtcatgtgt tattgtaata catgactttt agttaaaatt
    16921 tttctatcat ttaataatct attattcttg actattttat aataaaattc atatatggaa
    16981 atattaatac taaataatta atagttgata aaaaatagat aatatgctaa aagcaaaaac
    17041 taatttagag ccttgtaact gtttatttgc aattataaaa acatctttaa acatattgac
    17101 tataatataa aatattaact ataatacaaa acaatattaa ttaattttct ctacagctat
    17161 ccctggtata gttatatttc cacccatagt tgattcataa gttatagttc cagcacttat
    17221 acctcttata gttatgtaat catcctctaa tactcttgaa ggagttattg attttttata
    17281 actacatagt acaaccttat cataatttcc agacacagct aatcttattt gcacctcatc
    17341 accatcttca ataacttgaa taaccttacc ttcaaatttt acctttttgt acttataatc
    17401 atcaggtgtt ctagctaatt ggtcataagt aataccagta tcatatcctt tcttctcctc
    17461 ttcttctttt ttgcgttgtt cttcagcctt tttagcttct tcagcttttt tagcttcttc
    17521 agctttacgt tgattttcag cttcaatagc tttcttttcg tcgtctttca ttttgaacca
    17581 tggttcagca tcagacaatt tgctatttaa agtttctatt tctgtttttt gagaatctat
    17641 aactgatgtt tggttagaaa tgacctcctc atggtcttca gaacaagctg gtgaggatat
    17701 attgccaaaa aacacgccta aaataaatgc cagtagagca ataccctttg tgctcttaaa
    17761 gaatttaatt attttcttag agcttccttt tccttcatta ctaaattcgt taccctcatt
    17821 ttttttagaa aacataatac aatctccctt ttattagata tattttattt taaaagttaa
    17881 aaacttaaaa taactagttg actaaaaatt atatattcat attttcctaa aatatatttc
    17941 ccctaagcat aattttacaa aataaagtaa gaagattcaa tatattttga aaaaataaga
    18001 taaaaatgtc agattataat ataattttct tgaaggtgaa ttttatatat taaattaaac
    18061 ataaagtatt ttatttgtat aaaactatat tatgatgtaa ttaagtgact cagtgattta
    18121 gaggtggtaa aatatatata tatgaaagag cataaataaa ttgatataat aaaaaattaa
    18181 atattaaata ttaactataa taaaaaaata tctggtaata ggtataatat aaaaagaata
    18241 taccagaaag aaggtgaagt atgggagaac tacaaaaaat tccaggggta gggaaagcaa
    18301 cagaaaaatc tttaatcatg ttaggatata caactattaa atcacttaaa gatgcaaacc
    18361 cagcacaaat gtatgaaaag gaatgtttaa tgcgtggaca gcacatagac agatgtcagt
    18421 tatatgtata cagatgtgca gtatattttg catcaactga aaatccagaa ccagaaaaat
    18481 taaagtggtg gtattggaaa gattaaaaaa tcaaaaaagt tagacataaa aaagagttga
    18541 atatccgttt taaataataa aaactaatac tcaactcttt tttagtcgta aattttatgt
    18601 aaatctattt gtatggctaa atatacccat caataaatgt ataaatatac taatcaacaa
    18661 atgactaaac atactaccta acaaaggatt aaataaattc cttaacaaag cattctaaaa
    18721 caattaatta gaaactgttt cttcaagcca ttttctacat tgctcaatat ttaccatagg
    18781 gtcaaaacct tggtcttttc ccttatttaa aagcatatca caagggaaag tatcacattc
    18841 accacaatga ttaagttcct tactttcaca gcaagtttta accttacatt ctccacccca
    18901 aaatggtttt tccatagttg ggcaaccagt gcaatttact tgttcttttt ttgtacaact
    18961 gtcacaacag acaccacatc ttgattcaaa catattcaaa catcctttcc attatttaaa
    19021 aatattataa aatgatttta gatgaataca aaagtgcaaa tagttgattt taggtcatct
    19081 aaatgttaaa cataaaatta ttagtactgg taaatttata aaaactattc caaaaatctt
    19141 tttgaatatt tcaaaacaca ataaattact tcaaatcccc ttgaatatat tttacagcat
    19201 tatagattgc tttaaatctc tttgaatatt ctacaagact atagattagt ttaaatccct
    19261 ttgaatatat tttacaacat tatagattgt tttaaatctc tttgaatatt ctacaagact
    19321 atagattagt ttaaatccct ttgaatattt caaaacacca tagattactt taaatccctt
    19381 tgaatgttct ataacactat aaattacttt taacttttat ataaccataa agtttttatc
    19441 tggcacttta aatggagttt tatatcaaat ttgccttcca tcgaataacc aaacttaatt
    19501 taattacaca ttccaagagt caaatattca ttcaatatat ttgataattc tatgttatag
    19561 ctataattaa atatagatta ttaaagttaa atatttattt a
//
LOCUS       DAYAOX010000057        19531 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.057, whole genome shotgun sequence.
ACCESSION   DAYAOX010000057 DAYAOX010000000
VERSION     DAYAOX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.057"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(131..1000)
                     /locus_tag="ACGI57_001607"
     CDS             complement(131..1000)
                     /locus_tag="ACGI57_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9716687.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(1097..1954)
                     /locus_tag="ACGI57_001608"
     CDS             complement(1097..1954)
                     /locus_tag="ACGI57_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9716688.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(2169..4187)
                     /locus_tag="ACGI57_001609"
     CDS             complement(2169..4187)
                     /locus_tag="ACGI57_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFG9716689.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIINTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(4458..5234)
                     /locus_tag="ACGI57_001610"
     CDS             complement(4458..5234)
                     /locus_tag="ACGI57_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFG9716690.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLIDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(5291..6262)
                     /gene="asrC"
                     /locus_tag="ACGI57_001611"
     CDS             complement(5291..6262)
                     /gene="asrC"
                     /locus_tag="ACGI57_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HFG9716691.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(6275..7066)
                     /gene="asrB"
                     /locus_tag="ACGI57_001612"
     CDS             complement(6275..7066)
                     /gene="asrB"
                     /locus_tag="ACGI57_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HFG9716692.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(7067..8074)
                     /gene="asrA"
                     /locus_tag="ACGI57_001613"
     CDS             complement(7067..8074)
                     /gene="asrA"
                     /locus_tag="ACGI57_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HFG9716693.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            complement(8465..9154)
                     /locus_tag="ACGI57_001614"
     CDS             complement(8465..9154)
                     /locus_tag="ACGI57_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9716694.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(9217..10065)
                     /locus_tag="ACGI57_001615"
     CDS             complement(9217..10065)
                     /locus_tag="ACGI57_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9716695.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(10084..10971)
                     /locus_tag="ACGI57_001616"
     CDS             complement(10084..10971)
                     /locus_tag="ACGI57_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9716696.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(11029..11832)
                     /locus_tag="ACGI57_001617"
     CDS             complement(11029..11832)
                     /locus_tag="ACGI57_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFG9716697.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            complement(11992..12444)
                     /locus_tag="ACGI57_001618"
     CDS             complement(11992..12444)
                     /locus_tag="ACGI57_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFG9716698.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            complement(12574..14076)
                     /locus_tag="ACGI57_001619"
     CDS             complement(12574..14076)
                     /locus_tag="ACGI57_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HFG9716699.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            complement(14109..14990)
                     /locus_tag="ACGI57_001620"
     CDS             complement(14109..14990)
                     /locus_tag="ACGI57_001620"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFG9716700.1"
                     /translation="MRTTDMKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGL
                     YVGGSTGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGY
                     DAISAVTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENE
                     KIIGVKFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRA
                     RQIFELTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKE
                     ATDEMKSRAKEIYRKYF"
     gene            complement(15060..15731)
                     /locus_tag="ACGI57_001621"
     CDS             complement(15060..15731)
                     /locus_tag="ACGI57_001621"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFG9716701.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(16004..16495)
                     /locus_tag="ACGI57_001622"
     CDS             complement(16004..16495)
                     /locus_tag="ACGI57_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFG9716702.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            complement(16517..17881)
                     /locus_tag="ACGI57_001623"
     CDS             complement(16517..17881)
                     /locus_tag="ACGI57_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFG9716703.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            18300..19475
                     /locus_tag="ACGI57_001624"
     CDS             18300..19475
                     /locus_tag="ACGI57_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9716704.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
BASE COUNT         6406 a         3383 c         1890 g         7852 t
ORIGIN      
        1 gaatatacca ttttgttttt taataaaaaa tcatgctaca tcaatagtaa gcaactatta
       61 atgtagcatg attttttgta tctaaataat cttagtattt acctatcagt atagattgat
      121 atatcaaaaa ctatagtctt ctatattctt ctctttttag cttttggtta cttagatagt
      181 aatcaatttc atcaattaga cgttgtttat catcaggtaa tacacctttt aaattagcat
      241 aatttgatgc atgattactt ctaaatacaa ttttttcatt agcatctata ttctttatca
      301 tctctttaat ttcttgtaac acagccttat cctctagtat tgtaaactct cctgccttaa
      361 gtaaatcgta aagttcagta ttaggttcta ccattaaact aagtacaccc acataatctg
      421 gtgaaattgc tcctagcatt tttcctgtat caactgcatg ctgatgtgtt tttttagttc
      481 ctccaagacc tgctattaca gtggctgata atttaatccc acttttttta acttttctac
      541 caacttttat taattgttca ctactaaaac ctttcttaat atcttctaat acttcattgc
      601 ttccactttc aagccctaga taaatcattt taacaccaag ttttcttaat tctttaagtt
      661 catcatcaga ttttttctct atagatttag gagagccgta catagtaatt ctttcacact
      721 ctggaaaaac ttcttttata tataaaataa tatctcttaa tttctccatt ggtattatca
      781 atgcatctcc atctgctaag aatattcttt ctacatgctt ataatatctt ctaaatatat
      841 caatttcatt ttttattact tctagtggct ttattttaaa ttttttagat ttgtacatac
      901 tacaaaacgt acatttgtta tgagaacatc ctagtgtgac ttgtattata agactataag
      961 cctcacttgg tggtctgtaa agtggcatat cgtattccat attaaaatcc cccttgtaat
     1021 aaaatattca ttctattata ttattaatat ttactttaaa taataactta taaaattata
     1081 ttttttaaat tttagtttaa gatatattct ttaaaaattt aatgatacta ttggcagtaa
     1141 tattaagttg tttttcttga cttataatag catcattatc tcccttttgt tttccataat
     1201 ctccaaattg tgcatgattt cctccttcaa tctcaacata agttgtatca tcaggaagtt
     1261 tttgttttga ttctacaaga cttttaaaat tgactactcc atctttgctc ccccatatag
     1321 atataacttt ttttcctaat tctttcaact catcacccat tggataagaa gctaaaaata
     1381 caacgccttc aattaattta ttatcacgtg caaaatttga tgctgcaact cctcctaatg
     1441 aatgacctcc tattacccaa tgttctatgt tgttgtaaga atccataact ttctgtgcct
     1501 tattagttcc aagtatagct agattaagtg gcatctttac gattacaact tcatatccac
     1561 tttctgctat ttttcttgca agtggagcat aagattctat ttctacttta gctcctggat
     1621 acaaaattaa acctttagaa acttttcttc cttttggtgt aaatgaaatg ttttcattta
     1681 cagttacttc aactttacta ttgctgacta gagcttcttt tgccagcttt tgaggttcat
     1741 atgttttatt tatccatatg aaaaagccaa gcaaaatcaa tacaaatagt atacctattc
     1801 ccttaaatat cttattcttt atgttaaatt ttctattatt atttttatca tctttgttct
     1861 caacctcatc ataaagtaaa ggtctctcaa gttccatagt tatactaaat gtatcatatt
     1921 tcttattacg ctttaccttt acatctttac tcataaatct aacccccata atatttgaat
     1981 actattatag tttatcctaa gtaaattgtt ttttcaacca atgaaaaaaa tcaatatttt
     2041 aactattttc atataaaatt cataaatata tgttttttgt tttttatata ttattttctt
     2101 ttagtatttt ataaaacaaa tagccatata ctttatttta aatatatggc tattctataa
     2161 tttaatatct aagctgcttc ttttattgaa actttagtta tattacttat tctatacatt
     2221 tctttgacac catttagagt ttgcagtaag tttagacata ttatcttgtc acctagactt
     2281 ttagtattta taatactctc taaaattaca cttcttcctt catcatactt atctacattg
     2341 taatttgaaa tataattagt atctctcaat attttttcaa catcttcatc ttttatttta
     2401 tttttattaa catttatata tagattatac atattactta ttagtaattt ctgattttct
     2461 aaaaaatcct cttgatgttt atctttatat atagcattta tcaacataag tctatcttca
     2521 ataagtgatg gtatcaataa taaatttttt atatagtaaa catcagctac attttctata
     2581 gacttattga cttcatcaat tatattttta actattcctt tatacatttc tataacatgt
     2641 tgatagcctg ttttagcatc atatggtaat ataaacttat ttactattaa tgcaataatt
     2701 gctcctaatg ctacatatga aatcctgctt atggttaaaa catttgggtc tcccataact
     2761 actgctgaac caagagctga aacagttaca cataccatta attttctata atcaactaca
     2821 taagagttta tataccctgc taaaagaact attaacgacc ttaatgtact atcttttatt
     2881 attgaaaata atacaataaa tattacagct cctataaatg taccttctat tctttttcta
     2941 gacctttgta tacaattttc tgcgtaaggt tgtgttaatg aaaagacagt taacattatc
     3001 catcttccat ctcttaaatg gaaataatcc ataataaaac ccgctacagc tgtagctaaa
     3061 gcaatcttta ctgcataaga aaatcttata gaatttaaat taaagttttt cttatatatt
     3121 gatagtcgtt taaattcatg aggtatcttt gattttcttt cagctttctt ttcgtctctt
     3181 gattttactt ttttataatc tactaaaaac tcatataaat tttctaaaac actataaatt
     3241 tcttttaaat ttatattttt agtatcctga gcaataacat aatctctaaa tcctaatatc
     3301 tctttatcag ttatggtatc tttattttct aaacacatct taatatagtt tatttctttc
     3361 gttgctacaa ctagaacatc tgatttacta tttatgctac tttcttctaa aatatttttt
     3421 atatcttcat cttcaaattc tttactatcc tttttgtatc tttctaggat taaacttatt
     3481 ctttctaatg aaaataaaat attcattatg tctatacctc taatactgat ataaaagtca
     3541 tcttttctat tatcaaaaat aaccattttc aaatttttag atatagaatt tattctataa
     3601 ttacagctat ctatttttct catactagat ttaattatag aactatcatc attaatagta
     3661 ttttttaaaa gactaatttt ttctaataat tcatcacata tagatatcag ttctttatct
     3721 ccgacatttt tcatcctatt tctattcata acaaattgag caatcattat tataactgct
     3781 ccaacagcta aagccgacaa tctcatagga aattcacttt ttcctacagg tatacttacc
     3841 ataaatatat acattagacc aaagggtaca tatactgctc tttttaagtc ataactaaat
     3901 acatacccta ttataaataa tgctataaaa tttaataaaa ctcctaaata catattttgt
     3961 actgcaaaaa atgatagtat tcctaagatt atatttattg ctaaatactt cagtaaattt
     4021 ttaattggag aaatagttaa atctctttcc aagagcgtta gcatagctgt tactatacct
     4081 acacctataa gtgtattttt acttccaaat aaatatataa atatagaaat aaatgctatt
     4141 ataaaaataa acaactttgt ttttgatatt actagtttct ttgtcatcat atccccccta
     4201 aaaatttcaa caagaccaat tttatcatat ttgttatctt attcaaaatg cgctttttta
     4261 cggtaggact ttaacttttt ttatatttag aactatataa ttcatatata aacgtatttt
     4321 ttatatatat aaccgatata ttttcgtatg ctcccatatt ttttttgttt caaatatttt
     4381 ttcaaaaata taaaaataac tctctcaaca atactaaaaa tgcatttatc aagagagtta
     4441 taatttatac aataaattta attaagcttt tctttaccta atatataagt tccaagaccc
     4501 ataaataaag ctcctccaac aatatttcca agtgttacag ctactaaatt ataaatagct
     4561 cctccaatag taactgtatt tattgttggt gaaaataatg aaacactata aattgtcata
     4621 tttgctacac tatgctcaaa gcctgatgtt ataaaagcaa ataaacataa gaatatcata
     4681 attatttttg cagtatcact attagttcta aacgaacaca acactgatac acatactaga
     4741 atattacata aaatccctct aaagaaaaga gctgtaaatg gcatggatgc tttactagct
     4801 gctgtatttg caaaaaattc agctacaggt cctttgtcta ttagaccagt tcctacaaat
     4861 ataatgccta aaaccaaagc tccgattaaa ttacctaccc aactgtatgc ccatatttta
     4921 gaagtatctt ttatacttac acctttattt aacataccag cagacataac catattgttt
     4981 ccagtaaata actctgttcc cgtcattata accaaactta gtgctattgc aaatgataac
     5041 cccattacta tcttagtcat aggtgaacct gcatcagtca aaagtcctcc aatagtaaat
     5101 atcaaaagta tacctatacc tacataaagt cctgcaaacg cagaagatac caaatatttt
     5161 actttacttg tgtttaataa atttatctta tttatagctg cattagttaa tttatccaaa
     5221 gtttctttat gcataattcc ctcttccttc tattaaattt taatttattc aaatatatgt
     5281 tttactcaat ttatagatat ttaattccac tccagtatat gttttcgtac attttagtta
     5341 tttctggaag ctctacacct tcaagtgccc attttttaaa ttccataaat cctgttctat
     5401 caattatata tcctatatgt tcttttcctc ctggtgcatc tttatctata tacttttcta
     5461 catatctata agtattaagt attatcttta ttatgctgtc ctcatctacc cataaaagaa
     5521 aatcttctgc tagtctaggg tttttctttc ctgttctacc cattatagct aatctatagt
     5581 attttttctc atctctagtc catgcatttg ttgggcaatt tagcacacac tctccacagc
     5641 ctatacactt atctttatct cttaccacct tattattttc tgtttttaat gctccaacag
     5701 atatcttaga acattttttt acacatgctc cacaatttac acatctatcc ttttcatata
     5761 ttggtaaagc cataccaata attccaaagt cattcattct agcttttata caatcatttg
     5821 gacaaccagt taaagccact ttaaaatgta aatcatttgg aaatatagct ttttctattc
     5881 ttttagcaaa ttcagttgta ttatattgag cctttggaca gactttattt cctatacaag
     5941 ctgctatatt tcttgtacct gctgctggat aacctgcgtt tttctcactt tgatttatat
     6001 tcattttttc aataacaggt tgtataattt tatttatttc ttccatatct tccatattta
     6061 ttcctaatat ttcaaacccc tgtcttgtag ttatgtgcac ctgtccattg ccgtactcac
     6121 ttgcaatttt agaaactaca gctaaactct cagggtcaat aagacctcca ggtactctaa
     6181 ctcttaaaga agtctcatat tttgtttttg taattctata agcatttttc attacttttt
     6241 tggtatttaa atctcttatc acaaacttca ccccctaatc aataagattt ttagcatatg
     6301 aataatcaaa tactggtcca tctaaacata tgtaagtatc atccatttta caatgtccac
     6361 atttacctat tccacaacac atttttcttt cataagaaac ccaaatgttc ttttcatcta
     6421 aatctctttt taaaaattct tctactgtaa atttcatcat cattggaggt ccaactacaa
     6481 ctatagaagt attgtttata tctttcatat ccaattctgg tatgtatttt gttactaatc
     6541 ctacattacc actatatcct tcctctgctc catctacagt aactaaaata tcaagttttt
     6601 tactccatct ctttaaatca tctgcaaata aaatatcttt tggtgattta aatcctacta
     6661 ttaatttaaa cttttcgcat ttttctttat tattataaaa atattcaact attcctctta
     6721 caggggctaa tgcacttcct cctgccacta ctacaatttc tcttccttca taaagggata
     6781 catcaaatcc atttccataa ggacctctta taaaaaaaga atctccttct ttataattaa
     6841 acaactcgct agttactttt cctacatttc ttattgtaaa gtcaatataa tcctctccat
     6901 atcctgatac tgatattgga ctttcaccgt atttaggtat agatatctca taaaactttc
     6961 caggcaatac accttttgta ttgcagttta ctctaaaagt ccattcttta tcagtatgtt
     7021 taactatatc tagtattttt gcacttactg gtatatatgg gttcatttat acttcctcct
     7081 tactaattac taaatcattt attttttcta tacactttga aaatgatatg tattgcggac
     7141 aggcatcatc acatcttcca cagccaacac acatatgata tccgaatctt tttttaaaat
     7201 catgtatttt atgcatagtt ttaaatctca ttctttcgcc ttgttttttt ctaaaagaat
     7261 ttccacctgc tatatctgta tatccatcta cttgacaaga agcccaaacc cttcttctct
     7321 cacctacatt ttcgttttcc ttatagaaaa tatcctgcat tgtaaaacat gaacatgttg
     7381 gacatacaaa attacatcta ccacatccta tacatcttgt atcatattca tcccatattg
     7441 aatttcctat catttcattt gaatcaatat tctctggcac atctacagat attaaattat
     7501 cagttacata atcaacatta aattcagttg cttctccatt aaatacttca aattcactat
     7561 cctttatatc taagtacagt gtatctcctt ctatatttaa tccaattgaa taattatcag
     7621 ttttgttaga atccatgctc acacaaaaac agtttctaaa actttcctta caccctacaa
     7681 gtgcaaactt tactttatct ctgatatttt tataaaaata atctttttct tcaccattat
     7741 ttagatatat ttcatcaagt ctttttatac catttatgtc acaagctctt aagaaaatta
     7801 aaattttctt gttgtctaaa tcgctttcct taaactcttt ttcagtaaaa taaaacaata
     7861 cttgatttat gggaagtaca acttcttttg gtgaaaagtt tgctttctta gcaaattcca
     7921 tttcttcaaa agcgtttacc tccttgtatc ttataacatc agtatctgaa aaagtacctt
     7981 taaatgggat taaaactggt gcaaatatct tgtagtcttt ttttagataa ttcagacctt
     8041 cattgaactt agatgaatct aattttagtt tcatttttat cacctttctt atattttagt
     8101 tttacttact tgctatcttg tttaaatctt aacactttat tttctatgct tccgtgataa
     8161 ttatcacagt ttcaaaaata ataaaaaaaa tattaatgga gtttattaag ttctgttaat
     8221 atttttttat tatctacatt attcgatttc aaatctaatc acttaaatac aatcattaag
     8281 tacaataaaa ctactatcta ctcaattttt tgaataaaaa agaatcttat ttgttttttt
     8341 atacaattca atagggtaaa ataaaaatta ataaattaga caataaaaaa gaatctataa
     8401 atagattctc ttaataatat ttttatttaa ttaaaattct aataatactc ttacttaatc
     8461 ataattaatt atttttaaaa taccttgata attcagtttc tttaacataa aaacctttat
     8521 tgtcaatttt aataagttct tctttttgca atacctttat cattctggaa acagtctccc
     8581 ttttacagcc aagcatatca gctaaatatg taactgtaat atttacattt agtaaagtcc
     8641 attctccaca gcaaactcca aattctctac ttaacctata taattttgct gcaagttttt
     8701 tatctacttt tatggatata gaatttttca actgtctata taaacgtctt attcttcttt
     8761 ccatataaga aataatatcc tttgtaagtt gaaaatcatt ttgcataatt tctaaaaatt
     8821 cttcaatact ataataagct acaattgaat tttcaaatgc ttcacagcca acagtagagt
     8881 ttttagaagc gtcaagtgta atatcattta tgacttcccc actatttaat atgaaaatta
     8941 cttttctctc tccattttca cttatcttaa aaatagatac ctttccttct aaaagtatat
     9001 aaacactgtc taagctatct ttttcataaa ataaaatttc ttttttttgt aatcttttta
     9061 gtttaacttt ttctttaagt tgattcttcg ttttagtact taaattatta aaaattatta
     9121 ctttatcaat attataatct tttaatttag acatgtaata tctctccatt cctaaatttt
     9181 taagagagct ctataaaaca ttacaaattt cttattttat tcatttttaa acaattttat
     9241 agcatttgta gttttaattt tcctttcaat agcttcatta gaaagttctt ttactacctg
     9301 tgtatatact aaatcaataa tgaaaaattg tgctaattta gaagatattg aaccagtttc
     9361 cagtactgtt tctcccgata cataagataa gtgtacatct gaaatatcct ttaattcaga
     9421 atttttattt tcagtaattg atataatttt agcattattg gctcttgcaa gctttacagt
     9481 ttttataatc tcttctgttt caccagaatg tgaaattgca attattaaat ctccttcttc
     9541 cattatggaa gacatcatta tcattgtatg actactgtca aaacttacac aattaagacc
     9601 tattctcata aatttatagt ttgaatcctg tgctattata ccagaatatc caatacctat
     9661 gaagtaaact ttctttgcat ttattataag ccttgcgcag tcatgtacat ccttactatt
     9721 gattatctct actgtgttct ctaaagtggt tacatttgaa cttaaaagtt tttttgctgt
     9781 atccaaagct ggctcatcat tttgtatatt tttatttata atattttttt tattgatagt
     9841 tgatatttct tgtgctaaag taactttaaa atcctgtaat ccattaaaat tcattttttt
     9901 tacaaaccta gttatagttg cttcaccaat attgctctct ttagctattt gtgatattgg
     9961 tttataaaat acatcatcta tattttcttt tatataagct attaataatt tatctgattt
    10021 tgtgacttta aaagttggtg attccaattg ttctaaaata ctcattaaaa acccttctat
    10081 tctttatatt ttgtttaaaa aattatataa tgctccaagc attcctgcat cattttgtct
    10141 ctttgcaaat tctatattag tgtttttgta aactctctct ataagaacct cctttaatgc
    10201 tttgtctatt tttggtttta ggaacttttc ttgtgccata atcccaccac ctaaaattac
    10261 cacttctgga tttattacat aacatatatt tgcgattcca actgctaatg attttatcat
    10321 attatcgagt tcttttaaac aatcttcatc attattcttt gctaaatcaa aaattatttt
    10381 tccatttaag tcattttcat tcaaattttt tatttttgct acttttttaa ttagactgct
    10441 agtagatgcc aattcttgaa aagaacttcc atttacattc atatatccta cttcaccagc
    10501 actattacta aacccattta tcagtttatt atttattata atacatccac caattccagt
    10561 acctattgtt aaacatatag aagatgttgc atctttacct gctccaagcc acatttcacc
    10621 aagtccagca caattaacat cattttcaac ttcacatttt atattaaact ctttttctac
    10681 ttcttttttt aattgcatgc ccttatagtt tggaattaat tcttctaatg caaatagtat
    10741 tttaccttca actgggtcaa ccatacctgc agttgatata catataccac ttatttcatt
    10801 ttcctctata tattttttta ctatatcttt tattttggct aatattcctt ctccaccttt
    10861 ttcctttgct tcagtatcaa gacttgattt atcaattata aatccatctt ctctaataaa
    10921 accatatttt attgaagtac ccccaacatc tatacaaacg taatttttca taacatatct
    10981 ccatacaaat tatttttata aattaacaac tctatttaaa attttatatt acacaaatct
    11041 tttacttatc ttttccaatt cacagatata tttccttgct tctgataagt ctatagtatc
    11101 acttaataca cttttttctt tggttatatg atacttataa atatttttac ataagtcctc
    11161 tcctaacata ttaagacctt cttgagtgta atgaacatca tcaatagtta atcctctgtc
    11221 atgaaactgc atagcaattt tgcttcctac acataatata tcacattcac tacaagccga
    11281 ctcctgtgct gctgcaattc gtcttgttct aataaaatat tcatcttctc caagccaata
    11341 accagttaaa cttacaaaca ttttatcaat catacattct tctttaagcc tatacgcaat
    11401 tttttttaga ctttctttat agtacaatac atcactacca tattttgcat cactttcacc
    11461 ttgaatccaa ataacgtatt tattttctat attataatgt ttacttaagg tagtacagcc
    11521 tgtattaaat ttttctatag tgtcatttag aaaccagtta ttatcatgat tccagttttt
    11581 tattcttgaa ccatcttttg cattgtgaat aaaacaaact ttatttccag taagcttatt
    11641 ccattcattt gaaaatgcag gtgctatagt accatcacct tctgaagtct ctagctcaca
    11701 tctcatattt ataatttcat cagtactcaa atattcaaaa caattttcat ttggaatgat
    11761 gctctctttt ggatttccca atcctctagc attgctttgt cctattaata agaatacatc
    11821 tatattttcc ataagttaac tcctttacaa aattttaagc ttttaattat tttcaatttt
    11881 ctcataaaaa actaaggctg aaaatagtaa tctaactaaa atttacgaag tctcactaat
    11941 cgctgaaagt taaatcacta ttttcagccc tagtggttat ataaattatc attatctaac
    12001 caaaacctta aaaatagctt ttttgatatt ttctttttct aaaactttta ttcctgtcat
    12061 atgagcatct tccggataac aaactaaaaa atcacctttt ctaagttcca tgtatccatt
    12121 atttttacca tcaagaggca agaaatcgcc cttttcttca tatatttttt gttctaaatt
    12181 ttctataaaa tttatatcta ttcgttcaca tccatcaagc atcaaatgaa catctatata
    12241 cttcttatga gcttcccaga atctatcttc tttttcacaa gttttatact ctacaatatt
    12301 cacaaaaata tcatcaccat ctattgtata agtacctttt tcataatcta ttaatgaatt
    12361 atctttagca tattcaaaac atctaagtaa atcttcatgc aatgttgaat atgtcttact
    12421 atgatttata tttccaaata tcataagcac tatctcccta tataattata aagtctattt
    12481 ttatactttt atatacaaag tatattatac aatcttatag agtaactaaa ttatttatct
    12541 aatcactcta taagacaatt tatcatttaa atattatttt tcataataaa cagtagtctc
    12601 agcttttggt aaagatactt ttgctttaac tttagcttct attgcactaa ctattgtacc
    12661 taccactaca gaagtcgtga ttgttattag agaatatgcc caagaagtta cttctggcat
    12721 acggtatttt aaataaacaa ctaaaattgc tgatactatg aatccaacat atgctcccat
    12781 tttagtcgct tttttacaaa atgcaccaag tacaaatatt cctgctaaaa cacctagtac
    12841 tagccccata aaactattaa accattcata tgctgacttt atttctccat ttgctaatac
    12901 tattgcaaca gctatagaaa gtatacctac acctaatgat atatattgtg ctatcttagt
    12961 ttgcttttcc atacttatat cttttgttat gatagtttgt atatctaaag tccaacttgt
    13021 agcaactgaa ttaagccctg tcgataaagt tgattgagaa gctgcatata tcgctgcaag
    13081 tagtattcct gttataccca ctggtaattc ataagctata tatgatgcaa atacttggtc
    13141 ttgttgaact gtttgagcta aatctgggtt ttgagtatag tatataaata aacaagttcc
    13201 aattagataa aatactgttg caagtcccat tgataaaaca ccatttccat atgtcatttt
    13261 attaagttgc tttatatcag ttgttgtagt aaatctttga actatgtctt gactcgatac
    13321 atatgatgaa aaagtattaa gaccagctcc aacaaatatt ataaatgcac ttgtagatag
    13381 tatattttta tcaaacataa cttcattact tgctaagaac ttgtgtccag ttgtaagagt
    13441 atccattact gttccaaaac caccatttat acttgctatt aaatagaaca atgtccctgt
    13501 aacacctact attaatacca ttccttgtat aaaatcggtc cacaagacag cctttaatcc
    13561 acctgtatat gagtaaatta ttgctattac acccataact attatcagta catttacagg
    13621 tattcctgta agaccagcca gaactattga tggaagatac attatgatag acatacgtcc
    13681 tatttgatat attataaaca tcaaagctcc aagtactcta agacctttgc tttcgtatct
    13741 catttctaag tattcatatg ctgtatctat ttctaaccta ctatatagag gtaagaaaaa
    13801 ttttattgtc aaaggtattg ctataaacat acctaattgt gcaaaccaca aaatccaagt
    13861 accaccataa gaatttcctg ctaatgataa aaatgatatt ggacttaata atgtggcaaa
    13921 tattgatact gatgtaaccc accacggaat agttccatca cctttgaaga actcttttcc
    13981 cttcatttcc ttttttgaaa ataacaatcc tgcaaataaa actgccaata agtaaaccac
    14041 tagtattgca aaatctatcc aagtaaatcc taccatttga ctaatcctcc catactaaaa
    14101 gtattttatt aaaaatactt tctgtatatt tcttttgctc ttgatttcat ttcatcagtt
    14161 gcttctttca taggttgtct acagtatcca gcctcaacac cttgttcttc aagtagtaat
    14221 ttaattgttt gatataagcc atttcctaat atatctgtta ttaaatcatt agtcacatgt
    14281 tgtacttcaa gtgcttctga tattttttca ttcttagtta attcgaatat ttgtctagct
    14341 ctgacaccgt ttacattgaa tgtagaacca attgccccat ctacacctaa aactgtagct
    14401 ggtaacatca tttcatcaaa tcctgcaaat attaatttat ttgggaatgt ttttctcatt
    14461 ctctctaata agtagaaatc tgctgctgta aacttaacac ctattatttt ttcattttca
    14521 aataattctc caaactggtc taaactcata tcaacaccag ttaaaaatgg tatagagtat
    14581 attattaatc tattatctac agaatttatt attgtattat aatagtgttt tatttcttca
    14641 aaatcaaatt tatagtaaaa aggtgtaaca gcacttattg catcatatcc taaatcagtt
    14701 gtaaatttag caagttctac agcttctttt aaattaactg aaccaacttg agctattaat
    14761 tttatttctt ctttcacttc atctttagct atttcaaata ttcttttctt ttcatcagtt
    14821 gaaagcatga agttttcccc tgtacttcct cctacataaa gaccatctac tttacaaaca
    14881 tctatattgt gtctaattat ttgtcttaac cctttttcat ttatatttcc ttccttatca
    14941 aatgaaacta ataatgctga ataaattccc ttcatatctg ttgttctcat atctttacct
    15001 ccaattttaa ttttcaaata tagttaaata taataatttt ttaatttata aattaatttt
    15061 taattaattt caattgcttt aacaaatttt tctgttatta attgtggtct tgttattgct
    15121 gaacctacta ctgatgaatg aacacccaac tcaaagacct tttttaaatc ttgaggagta
    15181 tttacctttc cttcagctat tacaggtatt tctaaatctt taactaatct ttccattaat
    15241 acaaaatctg gaccttctaa tgttttagta tatggagtat atccagatag tgtagttgaa
    15301 acgcaatcta caccgtactc ttgtgctttg atagcctcat cataatttga aatatctgcc
    15361 attactagaa caccgttttc ttttatatac tttataagtt cttttaagtc ttcattattt
    15421 ggtctaactc tattggtagc atctaatgct atcatttcac accctgttgt taaaagttca
    15481 tcaacttctt ttttagttgg cgttatatat atatctgaat cttcatagtt tctctttata
    15541 atacctataa caggaagtcc agtaactttt tttatttcaa taatatcttc aactccttga
    15601 gctctaattc caacagctcc accttccata gcagccttag ccattctccc cattataaaa
    15661 ggactatgaa gaggttcatc ttctaaagct tggcatgata ctattaatct tccctttacc
    15721 ttatctagca ttttttaaat tacctcgttt ctgaaattta ttttcttgat ttatgtatta
    15781 ataataacat gaaaactttt tctttgcaat attttttcat atttttttct cgcatctata
    15841 tttttactga tttattttat atattagctt gttttttaca tttaaaagaa attttatttc
    15901 atttttttgg tgtttatttt cattttatat aactactgtt ataagtccat ttaaataatt
    15961 tcaataaaaa aacccatgta tactatacat aggtttccca atattattct aaatccatta
    16021 taatttttct tccacagcat ggacaaaatt cagcctctgt atctatctca ctttcacaat
    16081 aaccacaaat tataaaatca tcacattcat tttccaattc aaatttttta ccacattcag
    16141 cacaatattt atcatttaca tttccaacat aaccacactc acaagttaca ttactttctg
    16201 ccatttccat ttcattaatt tccatatttt tttcataaat taacttatca agttctagta
    16261 actcatcaca gaatttatca aaactattat cattaataga atcttctcta attttttgat
    16321 atgtcagtat tcccatttcc aagaatatct ttgctttctt ttcttgaata tcatcaatct
    16381 ctctatttaa ttttgatttt tctttatata attcaacttt aaattttcca ctaccaaact
    16441 ttttcttaac cttagaaaat ttatcatcta ctttattatt agtatttcct tccacagaaa
    16501 ttccctccaa tcatatttaa tcagctgaat ttagcttata tcctattaaa gatatcacaa
    16561 aactatatac aaatgaaatt acaatagctt gtaagacttt aaattgcatt attaaagttg
    16621 ttccataatt agttgtatta aagaaattaa tattactatc tagtataaat gtactaaata
    16681 aagccaatat tcccatcaaa aatgcataat atatactaaa tactattata ggtcttatac
    16741 tatctgtatt atacttaaat cttagcttgt atccatttaa taataatatt aacatagaca
    16801 atgctcccat tgcataaaac attaatttag tatctccaaa taaagatgaa gaacttaaag
    16861 taaacattga tactgttgag tttattatgg ttactgaaat tccatttgca aaagcccaca
    16921 tataactagc tatttgtggg agtatagtaa ataaatttaa tccatttgaa taactactca
    16981 tatctagttc atttaagtaa ctactatcag atatagttag taccaatagt attactaata
    17041 ctaaaacata acctaatatg aatgttctta tagaatttga aaaaagagat aaatacatat
    17101 tttccttttc ataacttttt ttataagtag ttatgtatgt acatataaac cctaatacaa
    17161 atccatttaa aagaacacta aaaaattcat aactatattc taatgcatat ccatattcta
    17221 acatactatg tgaactagtt tttacattag taaaaataga taaaatagct aaaactaatc
    17281 cataaaaaat tccaactcct agagaatttg ctaatacagt ttttgaatct ctacatcttt
    17341 ttctcataaa aattaggttt gatattaata ataccaaaat agggattata agtaatatca
    17401 acaaacctat attggcattt ataaacccag aacccatcat ggtagacata gatacactaa
    17461 tttgacccaa gttgagagct aggattatat gtgctggatt tattaaataa ctaatatcat
    17521 taaattgaat agttataata gctttaaata tcaatgcaat aataaacaat ataaatatgg
    17581 ttgtaaatgt tgttaaaaat acacctcttt tatttgcatg atatgaaagc tcttttattt
    17641 tagatttcaa gtctagtttt gtttcaagtg gtcttaatga tgctatttca taaagttcat
    17701 ttccacaaaa tttacagtaa ttatccttag tattattttc tttattgcaa ctagaacaaa
    17761 ttttttcttc tttactttta agagtgatat ggctattttt tatttttcta gttggttcac
    17821 ctgtcttttt ggaataatta ttatgtgaat aattcatatt atttattttc ttttcaccca
    17881 ttttttccct ccaagtttta ctattgtaaa ttatcaaata taactatatc atataaaact
    17941 taattatacc ttaattggat atatttatct attttatttg tttattgctg ttgtttttta
    18001 acaggtcttg atatttaaat agtttgttaa tgcgaacttt attatttata ttttattatg
    18061 cttaatttaa atttattcta ttctttattt tcagtagtaa actatctgta aatcttaatt
    18121 ataataaagt attaatcaat aaagttactc gacattatct aataaatttt atctatcaca
    18181 attttagatt gttacacaca atatattttg atataattaa atcataaacg aagtattcca
    18241 aatatctaat gatagaaagg aatacttatt tttatgcttt atttttaatg gaggcttata
    18301 tgaacttaga aaaaataaaa aaattttttg gatttcctct agacaaaaaa cacaaagaca
    18361 atgttgagaa agaactctgc aattatcttt atcatacggg taaaatcatt tgcataataa
    18421 tagtaatatc tcaaataatt atgatgatat caatctcagc tagagaagga ggcccttttg
    18481 tcacaccaag acgccaatgg tatttttata tgtatgtaac actattttgt gtatcttctg
    18541 tctttatgtt tgtattttct tacctttgga ataaaaaaaa ggattgctgt acgttttata
    18601 taaatttatg gataatttat gtgacttttt tctgcctatg gggttgtgta attactctac
    18661 ttgaccagtt aggtggaaat gacctaagtg tatttagtta tattatgtta gcagctgcag
    18721 ctttctcagt attaaaattt ggagaatcag taatagtttt tggaggatgc tttttgtttc
    18781 ttaatttatt cttacctggg attcaaacta taccaggcaa tgagtttagt aatattgtca
    18841 acacattttt tataacatcc cttgccattt ttgtttctac aatgctatat agaaatcgtg
    18901 tatttactta ctatgataaa cttttaatta gtcaacaata tgaagaaata tctcatataa
    18961 ataatttact taacgacaaa gttatgacag atgaccttac taaaatgaac aaccgtaggt
    19021 accttgaaga tattctacaa aaaaaattaa tcagccatgc aaatcaagaa cctataactg
    19081 gaatgatgat agatatagat tattttaaac aatttaatga taattatgga catcaaaagg
    19141 gagactattg tctacaaaat attgccaaag taattttaga ttttacaagt tctgaaaatg
    19201 aacatgctat acgatatggt ggtgaggaat tttttatatg tatttttaac tgtaatgagg
    19261 cagatgctaa acttaaatca gaatgtttac gaaaaattat agagtcctgc aattttgagc
    19321 gtgatgatgt tcctttaaaa tgtgtaactg ttagtattgg agtttataca aagaaaattg
    19381 gaaagacaat aacattaaat gagtttataa gtaattgtga taaggcccta tatactgcaa
    19441 aagaacaagg acgtaattgt gttgcatttt attaaaaggt tacatactat tacataaacc
    19501 tataagtatt atatcgcaaa ataaaagtaa t
//
LOCUS       DAYAOX010000058        19202 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.058, whole genome shotgun sequence.
ACCESSION   DAYAOX010000058 DAYAOX010000000
VERSION     DAYAOX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.058"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            203..784
                     /locus_tag="ACGI57_001625"
     CDS             203..784
                     /locus_tag="ACGI57_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9716705.1"
                     /translation="MMGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLD
                     TAAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPG
                     EISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVA
                     EIYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            823..1401
                     /locus_tag="ACGI57_001626"
     CDS             823..1401
                     /locus_tag="ACGI57_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9716706.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1487..2116
                     /locus_tag="ACGI57_001627"
     CDS             1487..2116
                     /locus_tag="ACGI57_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9716707.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2106..4865
                     /locus_tag="ACGI57_001628"
     CDS             2106..4865
                     /locus_tag="ACGI57_001628"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFG9716708.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            4952..6514
                     /locus_tag="ACGI57_001629"
     CDS             4952..6514
                     /locus_tag="ACGI57_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HFG9716709.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6515..7651
                     /locus_tag="ACGI57_001630"
     CDS             6515..7651
                     /locus_tag="ACGI57_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFG9716710.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7715..8791
                     /locus_tag="ACGI57_001631"
     CDS             7715..8791
                     /locus_tag="ACGI57_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFG9716711.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8769..10007
                     /locus_tag="ACGI57_001632"
     CDS             8769..10007
                     /locus_tag="ACGI57_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFG9716712.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10061..11149
                     /locus_tag="ACGI57_001633"
     CDS             10061..11149
                     /locus_tag="ACGI57_001633"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFG9716713.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11177..11968
                     /locus_tag="ACGI57_001634"
     CDS             11177..11968
                     /locus_tag="ACGI57_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFG9716714.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12083..12637)
                     /locus_tag="ACGI57_001635"
     CDS             complement(12083..12637)
                     /locus_tag="ACGI57_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFG9716715.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12833..14035
                     /locus_tag="ACGI57_001636"
     CDS             12833..14035
                     /locus_tag="ACGI57_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFG9716716.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14092..15072
                     /locus_tag="ACGI57_001637"
     CDS             14092..15072
                     /locus_tag="ACGI57_001637"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HFG9716717.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15315..16268
                     /locus_tag="ACGI57_001638"
     CDS             15315..16268
                     /locus_tag="ACGI57_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFG9716718.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16258..17214
                     /locus_tag="ACGI57_001639"
     CDS             16258..17214
                     /locus_tag="ACGI57_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFG9716719.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17211..17966
                     /locus_tag="ACGI57_001640"
     CDS             17211..17966
                     /locus_tag="ACGI57_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716720.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18058..19023
                     /locus_tag="ACGI57_001641"
     CDS             18058..19023
                     /locus_tag="ACGI57_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9716721.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7612 a         1943 c         3424 g         6223 t
ORIGIN      
        1 tattaaacat agcttttata catttaataa ggtatgtaaa atctatgtaa aaagtaagaa
       61 atataaacat ttttttaatt atattttaaa aatatcaagc aagttttctt cacaaatgaa
      121 taaaaaatgt tataatatga ttaaagaaaa agataaataa ttagcaagat ttgtttagct
      181 aaaattaaca agggagcgat aaattatggg tataacatta tcaaaaggcc aaaaggttga
      241 tttaactaaa ggaaatccag gtcttaaaaa tattttagta ggtttaggat gggatactaa
      301 caagtatgat ggtggatttg actttgattt agatacagca gcatttttaa caggagcaag
      361 tggaagtgtt actaatgatg gagattttgt attttacaac aatttaaaac atacatctgg
      421 agcagttgag catctaggag ataacagaac tggcgaaggt gatggagatg atgaacaaat
      481 agttgttgat ttatctaaga ttcctggaga aataagcaag atttctttta ctgttacaat
      541 tcatgatgca acagaaagaa gacaaaattt tggacaagta agtaattctt atataagaat
      601 agtagataaa gacacaaatg aagagttaat aaagtatgaa ttaggagaag attttagtat
      661 agaaacagct atagtagtag ctgaaatata taagcacaat ggagaatgga agtttaatgc
      721 actaggttct ggctttgaag gtggtcttgc tgcattatgt ggaaactttg gaataaactt
      781 ataatttcaa ctataacaat aaactaaagg gggatatttt ccatggctat agtattaaaa
      841 aaaggacaaa aaatcgattt aactaaggga aatccaggtc ttaaaaatat aaaacttgga
      901 ctaggatggg atacaaattc atttgatagt ggctatgatt atgatttaga tgtaagtgtt
      961 ttcatggttg gagaatctca aagagttgaa aaagatgaag attttatatt ctacaataac
     1021 ttaaaacatc catctggagc agttgaacac ttaggtgata atagaactgg tgaaggtgat
     1081 ggagatgatg aggaaatatt agtgaattta gagacaatgc caaagcatat acaaaagata
     1141 gctgtaacag ttacaatata tgaggctgct gaaagaagac aaaattttgg acaagtaagt
     1201 aactcatata taagagtatt aaattctgaa aatgatgaag aaatattaag atatgactta
     1261 ggtgaagaat ttagtataga aactgcaatt acagtatgtg agatatataa gtataatgga
     1321 gaatggaagt ttagtgcagt tggagcagga tttgaaggtg gcttagaagc actttgtaga
     1381 aactatggct taaatgttta atttaagcaa tataatatat taaatataaa taacctatac
     1441 aaataatgta taggttatat tatataaaat tttgtggagg tttagtatgt cgattaattt
     1501 atcaaaaggt gataaaatag atttaaaaaa atctaaccca ggactgagta acatacttgt
     1561 aggtctagga tgggaccctg tgcaacaatc tggtggaggt ttttttaaat cgttatttgg
     1621 tggaggacaa gctgatatag attgtgatgc atctgtattt atgcttaatc aagaaggtaa
     1681 attaagtgga ataaaagatt taatttattt tggaaatcta aaaagtgcct gtaaatcagt
     1741 tttgcataca ggagacaatc taactggtga gggtacaggt gatgatgaac aaatcctagt
     1801 taatttggat aaagttccat caaatattca taaattattg tttgtagtaa atatatacaa
     1861 ttgtgtagat agaaaacaac actttggaat gatagaaaat gcctatataa gagttgaaga
     1921 ccaaggtaat aagaaagaga tagcaaaata caatctatct gataattatt ctgaaaaaac
     1981 tactcttata gtaggagcta tatatagaaa agatggaagt tggcaattta aagcaattgg
     2041 tgaaggaaca aaagatgcgg gtcttaaaga agtaatgcaa aatttagaca ggattgagtg
     2101 tgcatatgga atttaataat gatatcaaat attataatgt agataaagaa gaagttacaa
     2161 aagacttatc tgttaatcct gaaaaaggtt tatcagaatc agaagtcaag actagaagag
     2221 aaaaatatgg tttgaatgaa tttactccaa aagaagaggg tagtttttgg gatgatttaa
     2281 aggaaagttt aagtgaacca atgatagtta tcttgattat tgcagcagta gtaagtgcag
     2341 taattgggga aactcatgat gcaataggga ttgtaggtgc tatagcaata ggtatagcaa
     2401 taggtatgat tacagaaggt cgttctaaga aagcagcaga ggctctttct aagttgactg
     2461 aaaacataga agtaaaagta ttgagagatg gggaagtaca tcaaatttct aagagtgaat
     2521 tggtaccagg tgatatagta tatattgaaa ctggtgacat ggtacctgct gatggtagac
     2581 ttatagaaag tataaacctt aaaattagag aagatatgtt aacaggagaa tctgatgacg
     2641 tatctaaaaa gtgtgatgta gtagtttcta tggaagagat agaatctaaa ggagcagtag
     2701 tagtacagga gccaatacct gcaaaacaga taaatatggt gtttggtggc acacttgtag
     2761 cttatggtag aggagctcta gttgtaactt caattggaga taatagtgaa atgggtaaaa
     2821 ttgctcaaaa cttatcagaa acagatgagc aaactccact tcagataaaa cttggaaatt
     2881 taggtggttt gatagccaaa gtttctagtg cagtagcagg attactattt atatttatgg
     2941 ttttccaaat gataagtaag aatgtattaa atgtagatat gtctggaata ttaccgtttt
     3001 tagaatcaat agacccagtt aaaacagcat ttacagtatg tgttgcgttg atagttgcag
     3061 cagtgccaga aggtcttcca actatgataa atatgacact tgcaataaca atgcaaaaaa
     3121 tggctaaaat aaatgcattg gttactaaaa aagaagcttg tgaaacgata ggttctgttt
     3181 ctgtaatatg ttcagataaa actggtacat taactgaaaa tagaatgact gtagaagttg
     3241 catatgttga tggcagatat attgaaagtt cagaagagga aataaatagt tattttgaag
     3301 aaaactgtat gataaactct acagctgatg tagagcacaa tgatggtgat attaagtatt
     3361 taggaagtgc tactgaatgt gcattgcttt tatattacaa aaacgtagat tatagacaag
     3421 ttagaaaaaa ttctaatata gtagctcaaa atccttttac ttcagattca aagagaatga
     3481 cttctgttat aggtcaagat aatcatcatg tactattatc aaaaggagca ccagaagtct
     3541 tacttgaatt atgttcacat attcaaagag gaaaagacat agttccatta actgaaaata
     3601 taaaacatga gattttagaa gaaataaaga aattgcaaat aaaatcaatg agaactttag
     3661 ggtttgcata taaagaaatc tcacaagctg aagaagaagc agcagtaact gctgaaagtg
     3721 atgctatgaa tgtaagtgca atggaaaaca atttagtatt tagtggtttt gttggtataa
     3781 gagacccact tagaaaagat gttatagagt ctgtaaatac agcaaataaa gctggtgttt
     3841 ctgtaaaaat gcttacagga gataatataa atacggcaag agctataggt gaagagttag
     3901 gcctattaaa aaataatatg agagtggttg aagcttcata tatagatact ttagatgatg
     3961 aagaattaaa acaagagata caaagtattt caatcgtggc aagaagtaaa ccagatagta
     4021 aaatgagaat agtttcagct ctgcaaaaga gcggagatgt tgttgcagta acaggtgatg
     4081 gtataaatga tgctccagca ctttcaaaag cagatgttgg tatagcaatg ggtatatctg
     4141 gtacagaggt atctaaaaac gcagcagaca taattttaac tgatgatagt tttagtacca
     4201 ttgtaaaagg tattaaatgg ggtagaggaa tttatgataa cttccaaaga ttcgttcaat
     4261 tccaattaac tgtaaatgta attgcatttt tagtggctat aatatctcaa gttatgggac
     4321 aagagatgcc atttactaca atacaattat tatgggtaaa catcattatg gatggaccac
     4381 cagcactagc attaggttta gagccagtta gggaccatgt gttaaataga aaacctgttg
     4441 atagaaaagc caatataatt tctaagtcaa tggtgtatac aatagtatta aatgcatttt
     4501 atataacagc aatactaatg ttacaatcta catttaattt cttaggagtt gatgaagcgc
     4561 ataagggaac agtaatgttt gcgttatttg cttttagtgc actgtttaat gcatttaact
     4621 gtagagagtt taatgcagat agtatcttcc ctaattttac acataacaaa ttagcactac
     4681 aaataatagt gttgacagga attgcacaag taatatttac tcagctattc caagatttct
     4741 ttaactctgt agcaatggat tttatgacat gggtaaaagt tttatgtgta gcagcaagtg
     4801 taattgtggt taatgaagtt gtaaaatgta taataagact ggtaaaacta aaaaagactg
     4861 cttaaaacaa attttttaga agaaaacaga ctgactcaaa atatgaattt tgggtcagtt
     4921 ctgaataaac atttaaattg ggaggatagt tatgggtgac tcaaaggtat ttgaaaaaat
     4981 attctccagc caatcagata gaggaaatta cactccttca aagggatatc taagttattt
     5041 tatttcgtat attggtttag aagatgaagt tttgtacaat ctcgagatat ttaagacaaa
     5101 acaaaatatt gatagcaaaa aagatattgc tttatttact gatgttatag caaatcctag
     5161 tgattttgat attataaatt attttaaaag tggacttcaa aaatatagaa ctagcatgga
     5221 agatgtagat ataaatattt tgggatttga agagattgat tataaaataa agcaagcaat
     5281 ggatagagtg ttaaaagaag aggaaaaaga atttactaat gatagagtta aacaaaattt
     5341 tatagtgaaa ataatggcat ggataaaaat ttatatcggt gctttagata taaataaaaa
     5401 tgaagctcca aaagtaatat tttatggaga tataaaaaaa catgaagttt atttactctt
     5461 aatattatac ttggcaggtt ttgatgtttt atatcttaat ccaaattcaa agagtaatat
     5521 agatatttta aaatcagaaa gatacaatat agagtttgaa gaagccaaca taatagaaga
     5581 aaaaatttct tttgaagaaa gggttatttt aggggaaaaa atagataaaa gttctgtaaa
     5641 aaaagctttt actgttgggg cagaagcatc aaagcgtata agtgaggaat tgttaaatga
     5701 tgcaggattt ataaaacctt ggcaactcca agatagaaaa ataaaaaatc tacttctaag
     5761 ttcaactgtt gatgaaatat caatatactg gaatcaacca cttaaattaa gaccaggatt
     5821 taagtttaat gatgctattg tagaagctcc aaattttctt tctaagatta atggaatata
     5881 taacgataag aatgaatata ttaagttttt agatttatta agagattcgg aaagttcaac
     5941 atttattgaa tttaatggag atgtagatag attttcaaaa gcttttacaa gagaagcttt
     6001 tagtttatcc tttttattgg attcaaaagg tgcaatagat aaaaactctg ttttaaataa
     6061 taaagattat tccatatcaa cactagcatt gaatcaacaa attatgattt tagagaaagt
     6121 ggaagagttg ttagaaggaa gtatgttttt gaatggttta agtggagaag acaagataaa
     6181 aggtctattt acagtacttc acatggataa gaaatttgtt catatgatga acaatttcga
     6241 ttactcactt ataaacccaa aattgattat atacatgtac aagagtattg tatttgataa
     6301 agaaatagtt tttcttatgc ttttacttag taagattgga tttgatatta tcatattatg
     6361 tccaggagga gaaaacaata tagagaatgt tataaataac caattgattg atgtacacag
     6421 attggataaa atggtatatg accttaaatt aaattcgctt gaaaatgata tacctttact
     6481 taaaaaaatt tttggaaaga ggagacgatt ttagatgggg ataaatataa atagtttttc
     6541 aaaagataag aactctgtag aggttactga aaatttgcct acagcagttc aagaagaaaa
     6601 cttcgatata atggaatata caaataataa aaaaaatgaa ctgagaaagt ctaaggaagt
     6661 tgaggcattg acatctctta tagaagtaga aaatccagat acgattttac agtttggtag
     6721 gaaagcatct gaaggagtag caagagtttc tgatagcctt ttaaatacaa taaaattaaa
     6781 tagaaatgaa gaaaattcta aaatgttggt tcatttaact aaaataatgg acaagtttga
     6841 tttagacgat tttcaagaaa ctaaggaacc aaatttcgtt caaaaacttt tcaaaaaagc
     6901 taataatgcc attgaaatga tgtttcaaaa gtatgaaaca ttgggaggag aagttgagaa
     6961 aattcaaatt gagcttgaac aatacgaaag agatatagca ctttctaata agcaaatagg
     7021 tgctatgtta aatgaaaact ttgagtttta caatgaactt caaaaatata ttgttgctgg
     7081 agaaatggca atagaagaaa tggataatga aatactacct ttctttaaac aaaaatcaga
     7141 gactagtgga gaccaaatgg atgttgtcaa ttatcaagaa ctattaaaag tttatgatat
     7201 gttaaatcaa agagtctatg atttaaggat agcagaaaat atagctatcc aaacaatacc
     7261 tatgcttaga ggtatgcaac ataataatta tggtttaatt agaaaaataa atagtgcatt
     7321 tgtcgtaact ttaccagtat ttaaacaatg tttatctcaa gctatattac ttaaaaagca
     7381 agaattacaa gcaaaatctc taaaagcact tgatgacaaa actaatgaat tattgcttag
     7441 aaatgcacaa aatgtaagta ctcaaagtgc acaaatagct agaatggctg gaacaagttc
     7501 tgttcaaata gaaacattgg aaaaaatgta caatactatc aagtctggta tagatgaaac
     7561 tatgagaata gaagaaaaca atagagcttt aattaaggat aatacaaaga gattggaaga
     7621 attaaatacg actataattt ataataagta gtattatatt aagggctaat ttgtataaag
     7681 ttggcccttt ctatgtatat gaggaggcta gacattgaaa tatttcgatt atatctgcaa
     7741 agatgatttg gagagaattt ttttgaagga gccagaagat tttagtgcta agactgaaaa
     7801 agatgtgttg aaatatgcac tgggcgcttt tttatacgtt ccagctactc aatataatat
     7861 gatttataag agtattatag gggatgttaa gggagttaga ccattagcta tttgtttaga
     7921 agatgctgtt ggagtaaatg gagagttgga ggctatagaa aatcttagat taattttaaa
     7981 aaatattagc aatgaaagta taactaataa agatggaata ccactaattt ttgtaagaat
     8041 aaaagatgtg gagcaactgt tgagaattaa agagattata attaaaaata gtcattttat
     8101 aacaggaata ttaattccta aagcaaattc ggagttaatt gaaaattgta tagaagcttt
     8161 agattttatg aatcttcaag atatgtatgt tattccgata gtagagacaa aagaatttat
     8221 atataatgaa aaaaaagaac ttagttttac taatttgtac aatgctattt taagacataa
     8281 gtcaagaata ttaagcatta gagtaggcat aactgatata ttagggatgt atggtataag
     8341 gagagataaa aacttttcta tatataataa tttgatttgt tcttcattta tattagatat
     8401 aatcacatat cttcaaagac cagagttaga tatacctata agtggtggag tatcagaatt
     8461 ttttgatatg acgaataagg atataagaaa taagtatata gaagaaatat tattggataa
     8521 atatcatgga ttaattggaa agaccgtaat tcatccaatg caaattcaaa ttgtacaagc
     8581 gctatctaca gtatcatatg aagattttac tgatgctttg gatatactgg atagtacaga
     8641 cagtaagtat ggtgtgagca agggtgtatt aggagagaga atgaatgaaa caaatccaca
     8701 ttttttatgg gctaaaaaaa cattgattct atctaaaata tatggtgtat taaataaagg
     8761 agtagactat gaagaattgc ttaaatttta attgtgagat tgacattatt gataatccat
     8821 tgaatttaga actaaatgat tttttagacg taggaataag aaataatagc aaaagacgtt
     8881 ttttgtttat ctcaaagaaa ctcggtaaac atttagcatg taagccaagt gagatggata
     8941 atttgggaaa attaatggct acaatttata atgaaaaaaa taaggaagaa tcaaatggta
     9001 ctgttatagc ttttgctgaa actggtacag ctgttgggca tagttttttt gattacttat
     9061 gtggagatta tgaatttatt catactacta gagagcagtt tgacgagtta gaaaatctgg
     9121 aatttttaga ggaacattcc catgcaacag accagaattt atactttggt atgcttaaca
     9181 actttaaatt tggagatgag ataatattgg ttgatgatga gattaccaca gccaatacat
     9241 gcatgaatat aattaggaaa attcaaagca tttatcctaa aaagaaatac actatatgct
     9301 caatactcaa ttgggtaagt gatgaaaatt taagtaaagt tactcgttta gaaagagagc
     9361 tagattgtaa gatagatttt gtatacttat ttaaaggaaa ctttaaattt tatatagatg
     9421 aagatattat ttcaaattac aacgataatt atagtattac taaattagag actatttcaa
     9481 gtgataaagt aaatttagta gtaaatcata tttatgttga tttagaagat tatattggaa
     9541 ataagaaata tgtaaaatat acaggaagat ttggaattaa tagaagagaa caatttagat
     9601 taaaagaaat cgttaagaga gaaagtaaaa aaataactat ggccagtaaa gaatctttaa
     9661 aaaatgaaaa agaaaagatt ctatttttag gtactgaaga atttatgtat atacctatgc
     9721 tgtttgcaaa agaaaaagaa aatcaatatg atatatacta tcattcaaca acaagaagtc
     9781 caattattaa tattgataag aataattatc ctattaaaag taaatttatg ctaaagagtt
     9841 tttataatat agatattcaa aattatattt acaatataga taaacataat ttttctaagt
     9901 gttatttatt tgtagagctg aataaaagtg aaaattctta tctagaattt attgatataa
     9961 ttaaaaaaac aagtataaaa gaattaacta tagtatgttg tagctaaagt gttttaacag
    10021 gataaaatat atcacgattt taagatagga gttgagtcat atgagagatt ttagcacgta
    10081 ttctaaagat gatgttattt ttttattaaa agatatatca aatttaattg aagaagaagg
    10141 aaataaagaa agagaattaa aaatacaaaa tggtagacat tattcagaaa tgataccaat
    10201 agagtatgaa gttagtgaag attatctgaa tttatatcat gagaaactaa gagaaaataa
    10261 agataaactt gcattttcta taggagttat gtgtgaaaaa ataattaaaa aaaatggaaa
    10321 tgatgtagta ctagtttcac ttgctagagc aggaacacct attggtattt tggcaaaaag
    10381 atatataaga agcaagtata atataagttt acctcactat acaatctcta taataagaga
    10441 taagggaata gatatgaatg caattgaata tatagtaaag aatcatccga gctcaaaaat
    10501 tcaattttta gatggatgga ctggtaaggg aactatatca aaagaacttg aaaaagcatg
    10561 tgatgaactt aatgttaaat ttaaaaaata ttttgactca acacttgctg tattagctga
    10621 ccctgctgga tattcaggac tttatggaat tagagaagat ttcttaatac caagtgcatg
    10681 tttaaattca acagtttctg gtttagtaag tagaactgta ctaagagagg atttaattgg
    10741 taaaaatgac ttccatggag ctaagtttta tagacattta aaagataaag atgaatctat
    10801 gaactatatc gaaactatag aagaatgttt taaaaatcaa tttaagaata tatctgatga
    10861 agttgaaaac tgggaaagtg acattataac aagagatgga tactttgatg ttttaaatat
    10921 aaaagaaaag tataacataa cagatattaa ctttatcaaa ccaggtgtag gggaaacgac
    10981 aagggtttta cttagaagag ttccgtataa gattcttgtt aaagacttaa atgataaatc
    11041 tttagaccat atattcatat tagctaagga gaaaaatgtt gaagttgaac aaatggattt
    11101 aaaagcctat aaatgttgtg gaataataaa aaatatgaaa gatatttagg aaatatatta
    11161 gacaggagaa tattgaatga ttgttttttc agatttagac aggagtatca tttactccaa
    11221 taaattttta aatgctgata gtaaatatac taatatagaa atttataggg aaaaagaaat
    11281 atcttatata tctttagata ctataaattt aataaagcaa atacaatatt atggtatgtt
    11341 tatacctaca actactagaa cagtggagca atttaagcgt atagaattta ataaatatgg
    11401 gatttatttt ccttggagta taacttcaaa tggaggagtt atacttaaag ataatgaaat
    11461 attgaaatca tggagtgaaa aaatagataa actaaaatgt gattatgagc ccattgaaag
    11521 tatgattcat aaatttaaag actatttgaa tgtagatgga ataactaatt ttaaagttgc
    11581 agaagataca tttttttata tagttgttga tttatcaaga tttaatttgg attcaatcaa
    11641 agaatataca aatatattgg aatcaaaaaa ttggaagttt tatgtgagtg gcagaaaaat
    11701 atattttata ccaaaagaaa tttcaaaaga aaatgctata aaatacttga ctaaagaatt
    11761 aggaatagaa tatttttatg cagttggaga ttctattatg gactatggta tgcttaatat
    11821 ctcaaataaa tcctatgtat tgaagcatgg tgatataaat aaaaatgaaa tagaaaattc
    11881 atttatatca agttctttca atggaatgag tggaactgaa gagatactat ctaacatttt
    11941 agacgagaac tgcctactta atatataatg actgatatat aaataataaa ctagaaaaat
    12001 ctgaaataaa ttatagtaaa gtttatcata ctaatttatt tcagattttt atcaatttat
    12061 atttaagtaa gaactcaata tgttaaaatg aaggtggagt cattgcccag actgagatac
    12121 atacctcatc atcataattt tcatacacat gtggtatagt actgttaaaa taaaaactat
    12181 ctccttcctc caatagatat tccttatttc caagtgttat cttcatttta ccttttatta
    12241 tatagccaca ttcttctcct tcgtgtgtca caaattcact gtttttttgt gtatgaccat
    12301 ctagtgtaac aagtataaat tctatttttt tcccagataa attaggaaca agaagttcat
    12361 aaaattttgt agaatcatta gttactattt tttttcgatt atttttcttt actactatat
    12421 cagagtcttc tcttccaatc tcttcaaaaa aataacctat atttatatca agagcttttg
    12481 atattttcca cattgctaca atagatggaa caaccatatt acgctcaatt tgacttatta
    12541 agcctgtact taaatctgaa ttttttgcta aagtagagat acttatattt ttagattttc
    12601 taagagatct aattttgctt ccaacatcta tattcattag atacaaaccc cctcagacat
    12661 gattataaca tataatttat aaagattaat aaaataaaaa aaaagtttta caatacaagt
    12721 attttttatg ctatattgaa ttttctgaaa agatgcatta taatagtatt aatattcaat
    12781 atattgaata ttgataacga tattttaatt taatgaaatg gaggtaatta agatgaagag
    12841 ttttgtaagt cctgataata tttgggtatt atgggcagtt gtaactggat gggcagcatt
    12901 tagtatttat ttggaacaaa agtacaattg ggcatcaaaa gtgtcgggag caataatagc
    12961 tcttgtagga gcaatgttat tatcaaatct aaatattata ccagtagagt ctgttgtata
    13021 tgaccaagta tggggatatg ttgttccttt agccatagct ttattgttat atcaatgtaa
    13081 cattaaaaag atatggaaag aaagtggaag attgctcatt atatttcttg taggttctgt
    13141 aggaactgta cttggagcta tgattggatt tttagcatta aaaaatgtag taccagattt
    13201 aaatattgtt gcagctatga tgacaggttc ttatataggt ggaaatgtaa actttgctgc
    13261 tatgtcaggg gcgtttgatg cgccaggaga attagtatca gcaacagttg tagcagataa
    13321 tcttttaatg gcattatatt tctttgtctt aatagctata ccatcaattg gattttttag
    13381 aaaacatttt aaacatccac atgtagatga aatggaaagt ataggtataa ctgaaggaga
    13441 aacagtagca gcaaattatt ggggaagaaa agaaatatct ttaaaagata tagcaatagc
    13501 cattggtagc gcctttataa ttgttgcagt atctgtagaa ctatcaactt ggtttaaagc
    13561 tataatacca ttatcaaatc cttttttagc aatgttaaat actttatttg gaaatcaata
    13621 tctaataata actactttga ctatgttgtt tgccacattt atgcctaatt tctttggtga
    13681 tataaaagga gcacaggaat tgggtacatt tttaatatat atattctttg ttgtagtagg
    13741 tgttcctgct tcaataacat taataattca aaaatcacca ttactacttt tatactgcgg
    13801 aataatggta ctaataaaca tgctagttac atttatagtt gcaaaaatat ttaagtttag
    13861 tttagaagaa gctattcttg catctaatgc aaatattggt ggtcctacaa cagcagcagc
    13921 tatggcaata tcaaaaggat ggtctaagct agtagggcca atacttatag ttggaacatt
    13981 tggatacata gtcggtaatt attttggtct tttagtagga aatattttga tataatttta
    14041 cgtgaaaaaa agttgtttat tagaaagatt tttatttaat tggaggttgt tatgattttc
    14101 gatggacatt cagatacttg ggcagatgta acaataagaa ggcttaacgg ggaaaaagat
    14161 gtatttagaa gaatccatct tgataatttt aaaagagcaa atatagaagg agctattttt
    14221 gtaatatgga tagacccacc atatgataaa gagcctgaaa aaagagttaa acaaattata
    14281 agttctataa aagatgaaat tgaagatatg aaagatgttg taaacatagt aaaaaaatat
    14341 tcagatatag ataaaggtat aagtgaaaac aaaataaata ttttaattgg tcttgaagga
    14401 ttaagccata ttggaagaaa tatagattta ctagattatt tttataaaga agttggtgca
    14461 agacatgcat cattaacttg gaatgaacag aatgaacttg ctacaggatg tttaggggat
    14521 gaaaatcgtg gtttaagtga aataggcaaa aaagcagtca ataaaatgga aaaattgggc
    14581 atgatagtag atgtgtcaca tttaaatgaa aaatcatttt gggatttaat gtctgttgca
    14641 agtaagccag ttatagcttc tcattctaat gctaggagtt tatgtgatgc taaaagaaat
    14701 cttacagata atcaattaaa agaaatttca aatacaggtg gattagttgg aataaatagt
    14761 tttagaggat ttactaatag taatcctaag aagcaagatt taatacattt ggttgaccat
    14821 atagactata tggtaaatct tataggtata gaacatgtat cttttggttt tgattttagt
    14881 gattacttag ggaaagagac tataggttct tttactggaa atgctagtgt gccaagtgta
    14941 gatggtcttg aaagcacaca tacagcacag aacatagtag tagagcttga aaatagaggt
    15001 tataaaaaga aagatataga gttaatctct agtggtaatt tttataggct ctttaaagat
    15061 atactaaaat aatataataa agaacttaaa tttataaaat agtatattta tacaaataat
    15121 atctacatat ttaaattggc gttcataatt tattaaattt taatgttact agaagtttca
    15181 attctagtaa cattttttat gttaaaaaca gatatagata tgtattgaca atgataatta
    15241 ttattgataa actataaaag tgaaaatgat aataattttc attttaatta ataaaagatt
    15301 tttaaggagg gagaatgaag aaaaggtatt tagtcatagg aataataatt ttgtcttatt
    15361 tttctttatt tataggagct gaagatataa atataatgca tatatttgcc aaaaatcagc
    15421 ataaacttat gatatttatc atgagtagaa taccaagatt aataagtata ttgattgctg
    15481 gtgtaggaat gagcatagca ggtcttataa tgcaacagat aagtaagaat aaatttgtat
    15541 cacctacaac aggtgctaca atagatgcag cacaatttgg gatagtaata tgtatgcttt
    15601 tagtaccaac tgcctctatt tttacaaaga caataatagc atttgtattt tctcttgtag
    15661 ggacttttac gtttatgaaa ataataggga agttacaatt taaaaatata atctttgtac
    15721 cattagtagg tataatgttt ggaaatataa tagggtctat gacggatttt atcgcgtata
    15781 aatacgattt gagtcaaaat gttagttctt ggatgcaagg tgacttttct atgatactta
    15841 aaggaaatta tgagatatta tatatcacaa taccccttat aatattagca tatatttatg
    15901 caaataaatt tactgttgtt ggtatgggta tggattttgc gacaaactta ggtcttagtt
    15961 acaaaagaat tgtcaatata ggtctgataa tagtagcact tgttacagta tgtgtagtcg
    16021 ttacagctgg taatatccct tttataggtc taatagttcc aaatatcgta tccttgtaca
    16081 tgggtgataa tataagagca agcatatggt atactggttt acttggagcg atatttgtac
    16141 ttatatgtga tatttttgga agaattatta tttatccata tgaaatatct atagggctta
    16201 cagttggagt tataggaagt atactattcc tttatttaat attaaggagg aatgtgaatg
    16261 aagcttagtc tcaatcaaaa aatttacttt gtaagtatat tactattcat atcaatggca
    16321 ctttttataa tttatggaat agacatgaat catttagaat atgcattatc acaaagaata
    16381 ccaaaaatat ttgctatggt attaggtgga ggatgtatag catttactac tgtagtgttt
    16441 caaacaatta caaacaatca aatattaaca ccaagtgtac taggtttaga ttcattgtat
    16501 gtaatgattc aaactataat agtattcata tttggttcaa gcagtagtct gattataaat
    16561 gaaaattata attttattat taatgtagct atgatgattg gagcttcatt gttattatat
    16621 aagacgttat ttgaaaaaaa taaaaataat attttctttt taatcctagt aggaatgata
    16681 tttggaacct tatttaaaag tgcaacaaca tttattcagg taatgataga ccctaatgaa
    16741 tttttagctt tacaaactag tataatggca agtttaaaca atataaacac taatgtattg
    16801 ttaatagcat ttataataat tattgccata atacctttta tatatgatga aataaagtat
    16861 ttagatgtat tgtcgttagg taaagaacaa gcaattaatt taggtgtaga ttttgataaa
    16921 gcagttaaaa aaatgataat attaatagca attttagtct caatatcaac agcacttatt
    16981 ggacctatga catttttagg acttttactt gctaatataa caagagaaat ttttaaaaca
    17041 tataagcata catacttgat ttcgggttct atgttgatag ggatgattac attgataata
    17101 ggtcaatttt ttatacaaca tgtatttaag tttgatacaa ctttaagtgt agtaataaat
    17161 tttataggag gaatttattt tattcaatta ctattaaagg gggcaaatag atgatagaga
    17221 taaaaaatat atttaaaaga tataagaata aaaatgttgt agatgatgtc tcttttagta
    17281 tagaaaaggg aaagataaca tcttttatag gaccaaatgg agctggaaaa agtacagtac
    17341 tatcaatagt tacaagactt ataggaggtg atggtggaga agtaataata gaaggaaaaa
    17401 gcttgactaa ttatagcaat aaagaattag caaaaaaaat agctattctt aaacaatcta
    17461 acaatataac tttaaagctt acaataagag aattagttgg atttggaaga tttccatata
    17521 gtgaaggtaa tctaactaaa gaggatgaaa attatattga tgaagcaata gaatatatga
    17581 agttgactga tatacaacat aagtatttag atgaattgag tggaggtcaa agacaaagag
    17641 cttatatagc aatggtaata gctcaagata cagagtacat attacttgat gaaccactga
    17701 acaatttgga tatgaaccat tccgtacaga tgatgaaggt acttagaggt ctttgtgatg
    17761 aattagataa gactatagta ttagtaatgc atgatataaa ttttgcatca tgttattcag
    17821 ataatatagt tgcattaaaa aatggaaaag ttgaaaaagt aggtcgtaca gatgagatag
    17881 taaatgaaaa agttttagaa gatatttatg agatgaattt taacattaag aatataaatg
    17941 gaaatcgaat ctgtatttat ttttaaaata ttgtatatga aaataaactt gctatatgat
    18001 tttttataaa gtttttaaat taaaataaag attaatagga gagaggaaaa tattgatatg
    18061 aataaaaaag cagcaatagt agcagcagta gctattatag gattagtgac agtgtttgca
    18121 ttaggtggaa gtaaaaagaa tgaatcaaaa acatcagaaa attcaaataa tactatgaag
    18181 ataactcata acttgggaga aacagatgta aaattaaatc ctaaaaaagt agtggtattt
    18241 gattattcgg ctttagatac aatggatgca ttaggtgtag ctgagacttt agtaggactt
    18301 ccaaaagcga gtttaccagc atccttagaa aaatataagg atgacaagta tgcagattta
    18361 ggaggattaa aagaaccaga tttagaaggt ataaagtcgg caaatccaga tttaattata
    18421 ataaatggaa gacaagaaga tttttatgag caattgtcaa aaatagcacc aacaataagt
    18481 acaagtaaag atgataagaa gtacttagaa tctgttaaaa ataatataga caaaatagca
    18541 aaaatatttg gagtagaaga aaaagcaaat caagaattta gtaaaattga aaagaaaata
    18601 gaaactttga ataaaaaagt aacagataaa aatttaaatg ctttaactat aatggtaaat
    18661 gaaggtaatt taagtgtatt tggtgaagaa tcaagattta gtatattata caatagtttt
    18721 ggatttgaaa ataaagataa aaatataaaa gaatcaagtc atggacaaaa tattactttt
    18781 gaatatatag ctaaacaaaa tccagaagtt atgttcgtta tagatagagg aatagctact
    18841 ggcagtgatg taaaagaaag ctcaactgca aaatctgttt tgaacaatga tataataaaa
    18901 tctatggatg catataagaa tgataatata atatatttag attcaccaac atggtatgta
    18961 aatgatggtg gtctaacatc tttaaataaa atgatagatg atgcttcaaa agcggtaaat
    19021 taataatccg atataaccaa aatgtatttt ttataaaatg attatcaaat aaactaggag
    19081 tttttaagaa ctcctagttt attttttgca taaaaaagat atagtttaaa caataatatt
    19141 atctataata agataaatct attcaaaatc taataaaata tgaagttata ctagacaaaa
    19201 ag
//
LOCUS       DAYAOX010000059        18871 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.059, whole genome shotgun sequence.
ACCESSION   DAYAOX010000059 DAYAOX010000000
VERSION     DAYAOX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.059"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..591)
                     /locus_tag="ACGI57_001642"
     CDS             complement(<1..591)
                     /locus_tag="ACGI57_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9716722.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
     gene            complement(921..2135)
                     /gene="dpaL"
                     /locus_tag="ACGI57_001643"
     CDS             complement(921..2135)
                     /gene="dpaL"
                     /locus_tag="ACGI57_001643"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFG9716723.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(3010..3297)
                     /locus_tag="ACGI57_001644"
     CDS             complement(3010..3297)
                     /locus_tag="ACGI57_001644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716724.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSM"
     gene            complement(3374..5179)
                     /locus_tag="ACGI57_001645"
     CDS             complement(3374..5179)
                     /locus_tag="ACGI57_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9716725.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(5575..5952)
                     /locus_tag="ACGI57_001646"
     CDS             complement(5575..5952)
                     /locus_tag="ACGI57_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFG9716726.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6857..7342
                     /locus_tag="ACGI57_001647"
     CDS             6857..7342
                     /locus_tag="ACGI57_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716727.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(7542..8081)
                     /locus_tag="ACGI57_001648"
     CDS             complement(7542..8081)
                     /locus_tag="ACGI57_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9716728.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(8074..8487)
                     /locus_tag="ACGI57_001649"
     CDS             complement(8074..8487)
                     /locus_tag="ACGI57_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9716729.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(8523..9644)
                     /locus_tag="ACGI57_001650"
     CDS             complement(8523..9644)
                     /locus_tag="ACGI57_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFG9716730.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            10232..11884
                     /locus_tag="ACGI57_001651"
     CDS             10232..11884
                     /locus_tag="ACGI57_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HFG9716731.1"
                     /translation="MNKKNLAILISGIMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            12183..12932
                     /locus_tag="ACGI57_001652"
     CDS             12183..12932
                     /locus_tag="ACGI57_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716732.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            12945..14972
                     /locus_tag="ACGI57_001653"
     CDS             12945..14972
                     /locus_tag="ACGI57_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9716733.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTRILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGIVDCIFAT
                     LVYKTALMQNLLGNSLSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKK"
     gene            15116..16699
                     /locus_tag="ACGI57_001654"
     CDS             15116..16699
                     /locus_tag="ACGI57_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9716734.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPDVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNISDNGVANTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISVSDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            16671..17291
                     /locus_tag="ACGI57_001655"
     CDS             16671..17291
                     /locus_tag="ACGI57_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9716735.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(17480..18406)
                     /locus_tag="ACGI57_001656"
     CDS             complement(17480..18406)
                     /locus_tag="ACGI57_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HFG9716736.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         6480 a         2952 c         2255 g         7184 t
ORIGIN      
        1 acctttttct ggattagcag agtgagctgg tttaccaaaa gtttctacta ctatttctcc
       61 tctacctctt tgacctatct ttaggtttaa ttctgaagat tcacctatta caacataatc
      121 aggttgaaca gccttactta tctctctagc tgaaacacct tcaaatattt cttcatgaac
      181 aacacctgct acatacaatt caccttcaaa gtctttgtta gtatcttctg caaagtatga
      241 aacagcagcc atcatagcac ttacttgccc cttcatatca gaagttcctc taccatatat
      301 tttcccatct actattctcg cttcaaaagg aggaacactc catttacttg catctggaac
      361 tggaactgta tctatatgcc cgtcaaataa tattttttta cctggtttat ttccttttat
      421 acatcctact atatttccat atctatctct tgaccaacta tcaaaaccta gtaaagcaaa
      481 attttcttct atagctttta ctacgttttc ttcttgacct gaataactag catttcttat
      541 taagtcttgg catacttcag ttaattgttt ttttcttgtt tcatttaaca ttttgatatt
      601 cccccatata atttttaatt tgttgacgtt ttattttgat atttcatttt tttaatttat
      661 tagcatttca cttaatctat tgatttttta tttctttata ttttatttat taaattttta
      721 tttagacttt attttaattt tatttggttt ttattttgaa tttttcattt gctttacttt
      781 tttatttgct ttactttttc atttatttag atttttcatt tgttttagtt tttattttat
      841 ttaaagtttt cacttattta agtttttatt ttccttaaat ttttcattta tttgttaaga
      901 ttttcttttc cttaagtctt ttatttactt tggcattcac cattccaaac tatatctcta
      961 tatttatcag ggtcagtatc tccttctgta cttattaata atactttaga gttctcatct
     1021 agtttcaaag cttctcttaa gtcttttaaa tcttcacttt caaggattga aactaatgct
     1081 ccagttgtaa ctgctccaga ttctccagag attactttct catctcctct tagagggttt
     1141 cctaatattc tcataccttt agcagatacc cagtctggtg ctgaaacgaa tgctgatgaa
     1201 taatttttta acacttcaaa acctatagtg tttggttctc cacaagcaag ccctgccatt
     1261 atagttggca tatctcctcc aacaaatctt ggttttccat ctccagcttc tgctgattta
     1321 tagtaacagt cagcctcatc agcctctact actactgtta ttgggcattc atcatatatt
     1381 gaagcaacat atccttgaac tgcaccagca agtgaaccaa ctcctgcttg aacaaatacg
     1441 tgtgttggtc tatctactcc atattctttt aattgttcta tagcttcttt agccatagtt
     1501 ccatatcctt gcattatcca tgctggtatt tcttcatatc cgtcccatgc tgtatcttga
     1561 accattactc catggtcatc agcttctgca taatcattag ctaatcttac tgcatcatca
     1621 tagtttaagt ctgttatact agcatctgca ccttctcctt tgattttatt taatctaaat
     1681 tcagaagaac cttttggcat taatacaaca gatttttgac ctaatttatt agctgtccaa
     1741 gcaactcctc taccatgatt tccatctgta gctgtaaaga aagttatttc tcctaatttt
     1801 tcttttattt cttttgaagt taatttatca taccctaact cagatatatc tgtatctaat
     1861 ctttgagcta agtatctacc catagagtaa gaaccaccta aaactttaaa tgcgtttaaa
     1921 ccaaatctat aagattcgtc tttaacatat attccgctta cgcctagtga ttttgctaag
     1981 ttatctaatt taactaaagg agttttttca tattgtggaa agctttcatg aaaagcttta
     2041 gcctttgcaa tctcttcatc ttttaaaaat tcaattcctt tttctttttc ctctgattta
     2101 ggtaagttat ttaatttcca ttttatttct ctcatttatt ttatattccc ctttcaaatt
     2161 tttcggtgtc attttaattt tttatctttg tgccttatag caaagcaatt accgtgccaa
     2221 tattatttat tctaaaaaca atttttttat gttggaatta catactttta taaaatttct
     2281 ttaaagttta taaattttga gtgtgaaaaa taagatttat aaaagaaaaa attctcatat
     2341 tgggaaattt ttatcaatac gagaattatc aattcgagaa atcgtttatt tatcttaatt
     2401 cttcctttat tatgctctta ttttttatat gtctattgtt ttgtcagata tattactact
     2461 tttattttct ataaatatat ggctataacc ttcttacgca taaactctat aaatttatat
     2521 actttatatt ctttattacc caaatattct ttattaccca aagcctccaa aatggcttaa
     2581 atcttttcat agcttcttac agaagtttac atgggcgtcc ccccaataag caaaagtgca
     2641 atatgtgtac cattgtgccc tacttgcgac tattgagctg tcgaaattcg caacttcgca
     2701 tttatctgat gaaatgcact atacaaaata ttcataacat ccacaacaga gttaaagtta
     2761 cctattcata ttcactgtaa aaatttcttc cacaggaaat gtaattttaa ccaatcctat
     2821 tgagacaaac tcaccactta tttttttgaa ctcacgcagt aatacatcac ttgcccgaaa
     2881 aaaagcctca ctttcatcat ctccagtaag tgctattgtg caatggggta cccaacgatt
     2941 ggggtggtac cattcccaac catttgaatc aaaatcttgt agtacctcat ataattctct
     3001 ctgaaactgc tacatactgc tattcattat aggtgataca aacaccgttt tagtatcatt
     3061 aaacatgcct actgaaccaa tgtaagcagg tattacctta tggttttgtg caaacccctt
     3121 tagcttgtct atacaacaag cctcatccac atcattgaaa caagccaaag taagatgagg
     3181 tcttgttttc cattctaaat acttcgtact aattttttca tcagcgattt ttttagataa
     3241 atcaaaaagt tgcttttccg tttttttatc ataatacagc tctatcgcat attgcataat
     3301 aacacctcca ccataaagta aaaataccta tctataaaaa cacacaaata cctatcattt
     3361 taatattata ttactactgt tcctcttcca attttctata tagtgtagcc aaacctatgc
     3421 ctaagtcttt tgctatcttc ttcttcgtct ttgtatctct tccatactta ttcaacaact
     3481 tgtttatgta agtcaactct aattctttaa ttgaaagtat cctttcttga ttctctacta
     3541 tccctccaac tatatcatct tcaaatatta tatttacatc cttattttta taaatatttc
     3601 cattaatatt ataatagttt aaaatatcta atggaagcat atctttatat attattccat
     3661 catctccaac caaattcatc atatattcaa ctgcattttc cagttccctt atatttcccg
     3721 gccaattata gttaatcaac atattcatta cattttcttc tattttattt aactgaatac
     3781 ctaattctaa agaatattta gaagcaaact tcatcattat tgcctcaata tcatcctttc
     3841 tttccctaag aggtggcaaa tttataggaa ttacattcaa tctatagtat aaatcatccc
     3901 taaacttacc ttcatttact aatttcaata agtttttatt tgtagcagca atcactctta
     3961 tatctaaatc tataagttta tttgacccaa ttctcgcaaa ttttctctct tgtaaaactc
     4021 taagtaactt tgcttgtaaa ggcattgaca aatctcctat ttcatcaaga aatataacac
     4081 cttcatttgc aagttcaaat ttacctactt tacctccact actagctcca ctaaatgcac
     4141 cttttatata gccaaatagc tcactttcaa ggagattttc tggaatagca gcacagttta
     4201 tcgcaataaa tggcttgttc catctgctac cttcagcatg tatagcccta gcaattagct
     4261 ctttacccgt accactttcc ccagttataa gtacagttga atttgacttt gctaatttct
     4321 tagttctctc ttttactttt aacattgctt ctgaatttcc aattatagac tctatatcac
     4381 tatttcccca gccactattc acttttccat gtcctttatg attgatatat gtcttgtcaa
     4441 atattattat tttgtcgtat tgatttatat atggatatac cggaattatt ttggcattta
     4501 tattataaat tttgttatca atatcaattt ctagtaattc atgtccattt gaggaagatt
     4561 caacagatac tatatttact atattttcta ttatattttt atctatatct aattttttta
     4621 gagctatatt gtttatataa gatattttgc tatttatgtc tagtattata acgccttttt
     4681 caacattttc aatgatttct atcatcatat ccatttttct actgctattt tttgacgctt
     4741 catgttcttc tattttcata gaaattaaat cgcatatatg attgacaaat tttaaataat
     4801 tatctatttt tgcacttatt tctacttttt tagttttatc tgtactaata gcaccaatta
     4861 ctcctatagt tcgattatta tactttattg gaactgctat tattacttta tttaggcatt
     4921 tcatgtaatg ggaacattct atacaaagct gattatcaca tatatccaat acgactaatt
     4981 cctgacctgt ctgtataaca ttgtcataaa taaagccctc agtaacaacg ctttcattta
     5041 tctttgattt atacagacct gtaccactaa ttcttattaa tctatcgtca actatttcaa
     5101 tatctacatc taacacacta gctactgttt cagtgtattt tatcatttct tgttgaaatt
     5161 cttttaattt acttaacata gtttccccca ttagaattta atttaccaaa ttatcagttt
     5221 agctatattt taacatatag tcactcttta aaatatatat aaaacattac ttatttatat
     5281 atttgcgtta aaacagacaa tattaattta taaaaagttt gttttatata gtaaccttaa
     5341 ttataatatg cagacaataa aataaatcac aatacaataa taaaaaaaag ctttcctcat
     5401 ataacaaata tatttttgac ataaaaatat acattatatg agaaaagctc aatagttaaa
     5461 tatttagata gatttttata tagacttttt taatataaac ttttttaatt gtagactttt
     5521 tttattagtt tttaattttt attagttttg ttattggttt tattgttata atacttataa
     5581 tactgccatt gcttcaattt ctaaaactgc atcttttggt aattgagcaa cttgtacaca
     5641 tgaccttgct ggtggatttt ctttaaaata tgtagcatat acttcattaa tatcattaaa
     5701 atcctttaaa tcttttacga atactgtagt cttaaatatt ttatccatac ttgtaccagc
     5761 ttgttctaat attgctttta cattatctaa agaagctttt gttgcttctt ttgggtcaga
     5821 tactaactcg ccagtcttca tatctattgg aagttgacct gatgtagaaa ctatgtttcc
     5881 aacttgaatt gcttgagaat atggtccaat tgctcctgga gctttctctg tagctattac
     5941 tacttttgac atgacttcac ctcctattat taagttatat ttacaggaaa ataaaatttc
     6001 ctaaacacca atattaatac gaacctttag attaaacttc aaatctaaaa cttcaaataa
     6061 tcaatactta gatgattatg tttatttgtg tcttgttaac ttttattaaa gcaattaacg
     6121 tgccaaactt tatttatata taaaattttg tgaagtcatt caaaatagtt acttctgaat
     6181 tttttcgtaa aaaaacgctc taaaattact tttttatgat gtcaattttt ttctcatatc
     6241 tagaaattta ccaacatgag aattcttgtt ttgagaattt acagattaaa gttatttttt
     6301 aaccttaaat tctgaacctc ccatgactaa agtcacaggg ttcctgcttc atagaatttt
     6361 gcataatcaa gttattacat atgaaagaac tatagttgac aatcccacta ttataaataa
     6421 taaaaccttt acccatacct tatttaccct agttatcata gagcctattg aaaatataac
     6481 agctaaatat aataataacc aaaaaattat caatatctac cactgttgct gaaaatccaa
     6541 aaaactacct tttgatagta ttacttaaca atctaattaa tctcataata aatttacaac
     6601 ttataaataa attgttatat tggccgtatc tcaacaactt tctcctttcc tagttttaaa
     6661 tcttttatag aattgtacca cgtctaagta aattttcata ctattaagtc ttatttctaa
     6721 taaatcatta gctctcattc tatgcccaat aatgtaagat tgcagtaatt ttattgattt
     6781 taatagtttt atttaatata cttatattaa ttattttcat atgaaattat tatttatata
     6841 gggggatttt tttaatatga gcaaaagtga attaacagca gaaacaacag aagaaatgtt
     6901 agaagtacta agtggtaaag attatgatat tgcatgtcat ttacatgaac ttggtaaatc
     6961 attagattgt aaaattgaac caaaaacagg tgctcgttct tacaaaatag tatattcaac
     7021 taagaaacca aaacgtagct tatttactat tgaatgtaat gaaaaaaaat ggagagttaa
     7081 agcaaatctt tttcatctaa atacatataa agatgctgtg gaggaatgct ctaaaactat
     7141 taaagatagt ataactaaaa ctcgtacttg taagaaatgt aattcaaagt gtattggagg
     7201 ttcttcgttt gaattagatg gaaagtctta cctgacttgc ataggaagtg gtcattattt
     7261 tgcaaatatg gaagaaatgg attggaaaaa cctagaaaaa ttaattacta aagaaaataa
     7321 tattatgcag gaatctgtat agttttttaa gggtaattat taaaatatat gagtaacaac
     7381 agatacttaa ataggcataa aaaaataaag ggtataataa ggatatagaa taaaaataaa
     7441 tgatatagca caaagaggct gtctcaaaat acagataagc ctctttttat tgttgaaatg
     7501 ataagaatat attcaatttt atacaatttt atacaaacat actacgccaa cttacccatt
     7561 tcatatgcct gtttcattgc cttacttccc ttaatatcac ctacattcca agcacctaca
     7621 ccatatacta tacctttttc cttagaacca tttagacatg atgtaaatcc tctaaatcct
     7681 tctattgttc tttccatatc agccacattt tcatctgctg ctgtaacgat aaaatagaaa
     7741 tctttatcac aaatttcagt atatctagag caagttctat caatcatagt tttcatttgc
     7801 gcattcatag tataaaaata aactggagtc gctaaaacaa tcacatctgc ctcaatcatt
     7861 ttttcaagaa tttctcccat gtcatctgtc tgtgaacaat ttgcttcttt ttcataacaa
     7921 gcaccacatc cagtacaata gttaatatct ttatctttta aaaatatctt ctccacttga
     7981 tttttagatt ctaatacccc ttgtataaac tggtcacaca ataaatcaga atttccacct
     8041 tttctaggac tagctgatac tattaataca tttttactca taagatactg cctccatttt
     8101 cttatttcta ctgctcttta atactttttc tttctcaata ataccttctt catatctatc
     8161 aatcttaaaa tttaatcgtt ccaaagtttt ttgcatttct tctattcttt ttgctagttg
     8221 gtttctctgc tctattaaaa gttcttttct agctttgata gtactgctcc cttgttgaaa
     8281 catagctaca tactcaatta atgtctcaat agaaagacct gcacctctca tacactttat
     8341 gaaattaacc cactcacaat cttcttctgt aaaagaccta attccacttt tatttctatt
     8401 tacaggtggt attaaaccaa ttcgttcata atatcgtaaa gtatccgctg aaagctcgta
     8461 ttttttacta acttctgaaa tcgtcataca tatcttctcc tcttgatatt gtatagattg
     8521 aattatttct caaaggtttc tgctacattc tttaaagcat ctataatttt aatgtgttga
     8581 ggacagcttt cttcacattg tttacattca atacaatctg aggcctttcc ataagtttta
     8641 gtataattat tatagtagac catctgtgtt gaaaatccag tattaagagc ttgtttttct
     8701 gcattgtaca acgcaaaata gtttggaata gcgatatttt ttggacagcc ttctacacaa
     8761 tactgacagg cagtacaagg aactaaaatt gattcgttga taatctctac tgcttcatct
     8821 ataatattat attcctcttg tacgaatggt ttgaaatttt gcatatatcc tgtattgtct
     8881 aataattgtt ccatatttga catcccactt aagaccatca taacattatc tttacttgct
     8941 gcaaaacgta ttgcccaaga tgaaattgac atatctggat tgtagtcatt caataatttc
     9001 tctgcttttt ctggtatctt tgcaagtgta cctcctttta ctggctccat aacaattaca
     9061 ggcttattat gcttctctgc cacctcgtag cattttctag attgtatgct ttcattatcc
     9121 caatctaaat aattaatctg taattgcacg aagtctactt ctggatgctc actcaagact
     9181 ttatccaata attctgcact atcatgaaaa gaaaaaccaa tattctttat ttttccttct
     9241 tttttctttt cttcaataaa cttaaagcta tcaaattttt tagctacttc ataatgtgac
     9301 acgcctatat tgtgtaaaag atagtagtca aaatagtcta caccacattt ttctaattgc
     9361 tcattaaaaa tggtttcttg ctcttctttt ttctttaaag ccattaatgg aagttttgta
     9421 gctatagtaa aactctctct tttgtgtctt tttactagag attctctaag tgctatctca
     9481 ctttttccca tgtgatacac ataggctgta tcaaaatatg taaatcccct ttctaaaaaa
     9541 gtgtctacca ttttatttaa atgttccata tcaatagttg taacatcatt ttcatctttt
     9601 attggtaaac gcattaatcc aaatcctaat ttttttgcct ccattgtttt acttccccct
     9661 ttaaaacttg tttgtttttt ctctctactt taactattct tacaagtata taattcttgt
     9721 ataattatac tttactactt ggagttaact tcaagtcaat agattttttt atttttaaga
     9781 caaatattat aaaaatacag ttctttaata ttaatccatt acatgtttct ttcaccctcc
     9841 aattctcaaa cttcacaaac tccaacatgt aaagaaatat atactttaag aaattcagtc
     9901 cattaatatt actacaaata ctttttagat atgtttaaaa agaaatttca cctaaaagat
     9961 ttagaaatag gtcttgtgtc tattttaaaa tatataaaac agtggtaaag acttatttat
    10021 aagataaaaa atgaatagtg tatacccttt ttaaaaagtt ttttcctttt tatatttgtg
    10081 atacaatgtt aaatataaaa aggaaaattt tgtattaaaa aaatcttatt tgttgatttt
    10141 gtaacttcaa actcagcatc aatttgattt taagattata ttttattaag tatgctaaat
    10201 aacaaatttt taaagattgt gaggtaaaaa tatgaataag aaaaatttag ctatattaat
    10261 atcaggtatt atgttttttt ctagttcttc agcaattttt gcagacaata ctacaaagca
    10321 agaaagatta ataggtaaaa caagatatga aacagcagta gaagtaagta aattgggatg
    10381 ggttcaatca aaaacagcca ttatagttaa tggtaattct attcaaagtg cgctttgtgc
    10441 aaatccattt gctaagctaa agaatgcacc tatactttta gtaaacaaca acagtattga
    10501 aaattcaact aaggctgagc ttaaaaggct tggtgtagat aatgtgtaca ttgtagatag
    10561 tggaaatagc atttcttcta aagtagaaaa tgaaataaaa tcacttaaca taaaaataaa
    10621 taaaatagta ggaaacaata tatatgaaat gtctacaaat gtacttaaag aaatagataa
    10681 gataaaaaag gtagaaaatg ttgcagttgt aaatggacta aagggactat ctgatgcagt
    10741 tagtatagct tctccagcag caatacataa tatgcctata atattagtat cagatgaaaa
    10801 cagcaatgat agtagtataa atttcttaaa caataggtct ataaaaaaat cttacatagt
    10861 tggaggaaat aagattttat cagacaatac agtaaagaaa tatccaaaca gagaaagaat
    10921 cagtggttca gatagaaaca atactaatgc aaaaattatt gataaatttt ataaagataa
    10981 aaaaataaat aatgtatttg tagctaaaga tggtattaac caagaaaatg cattagtaga
    11041 ttcattatca ataggagtac tagctgctaa agaaaattct cctataatga tatctaatgg
    11101 tagccttgta aatagtcaaa aagaatttat taaaaataaa gatatagatg aaataacaca
    11161 agtaggagga ggatataatc aaaaagcatt tgaagatatt ttaaaactaa aaaatgtgaa
    11221 cataagaaat aaaactaaaa aaatatcttt aatacaggct aaagaaatat taaaacaagc
    11281 tcttccagga agcaatattt tagtttgtta ttatgatgaa gatgataatg aatatgactg
    11341 caaagttata aaggataata tcctatatga gatagaaatt gatgcctact ctggtaaaat
    11401 aatagaaatt gaaaaagatg atgacttaaa tgataataat ttaggtacac aaaatatatc
    11461 attggataaa gctaagtcta tagcattaga tgctgttcct aatggtaatt taatctattg
    11521 taaatatgat gctgaagata acgaatatga ggctaaagta ataaaaaata agaaaacata
    11581 tgaattagag ataagtgcat ttgatggaag aattgtagat attgacatgg acaatgattc
    11641 tgatgatgac tttgatgata atgataagaa taactctaat actaatgaca acagttctat
    11701 tattagtgct gaaaaagcta aatcaataat gctagataaa gtgcctggtg ggaaaatagt
    11761 aaaatttaag tatgatgaag atgacaaaga atatgaaggt gaaataataa aaggaaaaac
    11821 tgaatatgag ataacaatta atgcaattac tggtaaaata ttagaatttg aaatagatga
    11881 ttaataaaaa atatatattc tattaattat tacaagcaat aaagccataa atattgaaaa
    11941 tttcaatatt tatggcttta ttttattttt tttaatttaa atccacatta tgccattata
    12001 tatacctatg cactctttta aattaaaatc ttgtccatca tcccaaacct aataaagtta
    12061 gattttaaac aatatattgt gatataagta atttgtgtag aattattgcg tgatttactc
    12121 atgaagaaac aataaggtta atatataatt tgactatatt aagacgaaag gagacatata
    12181 caatgaatac aatattaaaa gtaaataaca taactaaaac ttataataaa gggcaacagc
    12241 cgagtttaga taaggtatcc tttaatgtat cagaaggtga atttataggt ataatgggag
    12301 cttctggctc tgggaaaaca actctactta atgtattatc tacaattgat aaaccaacaa
    12361 gtggagatat tttcataaat aatataaatt tgaaatcgct taatgacact tgtgctgctg
    12421 attttcgtaa ggataattta ggctttatct ttcaagagta ttttttacta gatagtctta
    12481 caattttcga gaatatttca gtacctctta cacttcagaa actcaatcca gataaaatag
    12541 aaaacatggt aaagagtcta gctgaacgat ttggcatagc ttctcaatta aataaatacc
    12601 caaatgaatt atctggtggg caaagacaga gagcttcagc agttagagca atagtaaaac
    12661 atcctagtat tctctttgca gatgaaccaa caggagcttt ggattctaat tcagctactg
    12721 aactactcca ctcactacag gaagcaaatg aggagttgaa aacaacaatt ttaatggtaa
    12781 cacatgatgc ctatgtagca agttttgcaa atagaatttt gatttttaaa gatggtcata
    12841 tcattaaaga attaaataaa caacagaata atagacatac attttatgag ttgattattc
    12901 aagaaattgc aaaattggat actaaaagat aggaggataa tttaatgaac attttttata
    12961 cggcaataaa gaatcttaaa aaaaactttt cattctattc tctatatcta ctttcagtct
    13021 cttttgttat tactgtattt ttcgcattta cttcattttc tgttaataag ataatgctag
    13081 aaaaaatttc aactgatggt agagttgaat ctatgtgtaa tgttatttcg atatttttga
    13141 tggcatttgt catcttctat atggcatact caaatcgatt cttcttgaga cgtcgtacaa
    13201 aagaattagg tatatatgct ttacttggct atcgtaagtt tacaatatta tcgctactta
    13261 cctttgaaaa tgtattaatt tgtagtggag cttttattat tggattggta ttaggagcat
    13321 tggcacataa gggtattgtc tttggaatta cagcactact taatttatca attgataatt
    13381 cacagatacc attttttaat ataaatgcaa taaataaaac aattatcttt atagcattag
    13441 ttgttttagt cttagctgta tcaaatggta aattttttta taagactcca cttgtaaaat
    13501 tgataagata tgaaaaaagt gcagaaaaaa aattaaagtt cagaaaaatt ccagccttta
    13561 tggggtttat tatgatttta agcgggtatt gtttatcact agatattctt cgtggagcta
    13621 aatcagtatg ggtaagtatt ggattttatc aaatgggctt gataactatg attatcattg
    13681 taattggtac agttctcttt atcaattcct ttcttccata tattatgcaa aatagtaaaa
    13741 aaaataaacg tactttctac acagatatga agataatagc tattccaaac tttatctatc
    13801 gtatacgttc aaactctaaa actctaatta tgcttacatt actctctgct actactttga
    13861 cagtttcaag tgttatggca ctgtccttat actaccctat tgcatctgtt tcgcgtattg
    13921 caccttcaga gatagaattt agagtagaaa aaaataaaca aatagaagat gtaaaacaaa
    13981 ttgtcagtca atatacgtct aatacagatg atattatttt tacacagaca aatatttata
    14041 aggtaaaatc ttcatcaaaa gaccttccat ttgaatatga aattggtaca tctaagaata
    14101 atttatccaa tagtagttct tctaaagaag ttatttcaag agaatcagga tttgaatgta
    14161 tatctttttc tgattattat actttattaa aggcacaaaa tcgaacaaag ctactaaaac
    14221 aacttacccc tttaagggat aatgaatgta ttttaatgaa atatcaacca aataatgatg
    14281 gatatagtga aaatggaacg atttatccat tacttgttaa tggtaaagaa acacctgtag
    14341 ctgtaaggga aacatcatta aacaatgcta tttcttttgc caatagcgtt ggaacactta
    14401 tcgttaatga tagtatatat gagcaaatag cacaaaataa attaccaatg acaagaattt
    14461 taagtattaa tggtaaatca attaaaaata atgaaaattt atatagtaat atttcagatt
    14521 atttagatgg aagcccttat ttacaaggat attctcacag gataaatgat ttattatcaa
    14581 ttaatagttc tacatttctg ttaatcggat ttcttgtggt attattcttt attgctgttg
    14641 gaagtatttt atactttaac aatatatcag caatttcaga ttcaacatct gattataaaa
    14701 ttttacagaa aatgggttat actaatatac aaattaaaaa aattattaaa aaacaagtat
    14761 tgccattttt taatattccg tttttacttg gtatagtgga ctgtatattt gctaccttgg
    14821 tttataagac tgctttgatg cagaatttac ttggaaattc actttcacaa tacatacctg
    14881 tgattattgc aattttctta acttctgtag tatatttgat atattacata cttactatta
    14941 aatcctgttg taaaattgta ttaaaaaaat aatatttgcc aaatcgtcaa gcttaaaggt
    15001 gtgacgattt ggtatacttc atttataagt atttgatata atttaaaact ataaattctt
    15061 aagtttgatt tagtcataaa attaatataa ttaactcata aggaggtgta taagattgac
    15121 aaattttaat ttaaaaatac tatttttagg aaactacatt ctatcacttc tcttcatggg
    15181 aatatctaca agcactgaaa cttatatgaa tattcaaagc attattccct taataatttc
    15241 aacattatta ctcatagaca tcacatatat aacctcttgt aatgtccgag aaaataacat
    15301 tatttcattg ttttgtggat tactagcact agacagttgg tatttactct tatcatttgt
    15361 aaccacacca ataggcacta ttatttttaa aatattaagc acagttatag tgtatgtctc
    15421 tattaaattt atatttttat ttttgtttca aggtagtgga tacaatttcc gtaagataac
    15481 agacatttta ttgcttatca cctgcattgg tgcaattgta ggagcattta tttctaacag
    15541 aacgtatgct tgtatgtacg gaatacagtt tgttgttagt ataataagct ttttctatat
    15601 tattttttat catttgaaaa gggttgtttt tgtcataaaa agcgaaaaaa aacctatatc
    15661 gatttcagta tttataacaa ttataggatt ccttacttat tattttttta cactaagaat
    15721 atcaaatcat attggtaatt ttggaatcta tctaactgta ctggtatttt ttatgagtat
    15781 tcatggtatt gcacttaaag aaaatgatag ttttcctatt tcaacagttt ttaacataaa
    15841 acaaagggct atatttcttt ttataagtat tttagtaatt agcttggtcg tactatttgc
    15901 caatgggaat ttttctataa tattaatagc tataaatgtt ttatttatgc tcattttcct
    15961 ctgtaacatt attttggagt ttaaccttag agatgaaaaa aataaagtag ttaaaaatag
    16021 caagtatact tttgcattag aaaaattaca acaagaagaa caactaaaaa cagagttttc
    16081 taatttttta catgatgaca tattacagaa cctgctttca ataaaaaaca tgatgagtaa
    16141 gtcatatcgc ccagatgttc aagatattat atatgaaaca cttaacaatc taaatacatg
    16201 tataaggaag caaatgcaag actatcatcc tgttatttta aaaaatttaa cagtaaaaga
    16261 aaattatcag aatttaattg aaagtgtttc ttcatccttt ccacaaaaaa atatttgtgt
    16321 ttcatttaag tgttctgata ctttattctt agttgaacca tatgatatat tgatatatcg
    16381 actgattaaa gaattattga caaatgtgta taaacattca aatggtagcc atgcatggat
    16441 agctttagca ctagaaaaaa atataataaa attaaatatt agtgataatg gtgtggcaaa
    16501 tacatctttt aaaacgcctt tagatataac taaaattact tttgatacaa taaaacacaa
    16561 agggattata tcaattaaag aacaagtaga aaatctagga ggaacgataa gtgtttctga
    16621 taatatacct catggagttt gtgtacagat tacaatccct atgaaaggag atggttctta
    16681 taaatatttt gttagttgat gaccatatgc tttttgctaa aagcttgtca attgcactag
    16741 aagaatacaa agaaatcgaa cagttttatt ccacacagga catccataat ctttcagaaa
    16801 tgattcaaga taaaaaaata gacattgtct taatggatat aaaccttaga ggcttaagtg
    16861 aatgtgatgg cttaaaagta gcttataatt tattagagac taattcaact attaaaataa
    16921 taatcttgac tggatacgac ctcccagttt ataaacatga ggctaaaaaa ataggtatct
    16981 atggattcct caataagaat attagccctg atagtttaat tgattcatta ttaaaagtct
    17041 atcaaggggt aacttgtttt ccatctgaaa atactgaagt aattattgaa gatttaacta
    17101 ctatggagaa aagaatttta cagcttatct gtagtggtaa aaaaagaaaa ataattgctg
    17161 atgaattgta tataagtgaa agaacattat caaatcattt acaacacata tatgacaagc
    17221 tagatgtttc atcatcagtt gaggctatta caaaagctat taaaatgggt tatatttctc
    17281 cagtatatta aaaaaagtga gtgtctcaaa ataaatcttc taactcataa agcactctcc
    17341 tttgtattaa atcaaatata ttttcatcat ttaaacaatt aagaagaggc ttctctctat
    17401 tttgagacaa ctccatttta atacactatt tatatttata tataaacata ttttaaacaa
    17461 ctaaaaagca actaatatct caatacatat cttcataaca catatcacca taattacaag
    17521 cagataaaga actctcctca tttaaatacg actcaaattc atacccaaac agaattgttg
    17581 gttctatata tatttccata tctgtatatc tccatcttct tacctttgcc tctataacct
    17641 tgtaaaaatc ttctaaacta tacccttctt ttaatctttt atctataagt gccttagtta
    17701 tactcaaatc agctttaaaa ttttttcctg attcactatt taattttctt ataataattt
    17761 catgtatgct ttctggattt ttgtaagtag aatcttcagt caaatcttca tccgaatttt
    17821 caaaggtatt tttatttaaa ttctttttat ttaaattatt ttttaataat tcttttttct
    17881 ttgtctcatt ttctgtttca tacccagtat gaattatcaa atcataatct ttgtctatac
    17941 tctcttgatt tatacggctg tatatgttgg tattactgtt cttaaacaac tcatttctat
    18001 gtttatattt cattgttttt tggttattgt taatagttgc atcaatgtat ttctctatat
    18061 ttttgaaaat atcaccattc tttgttacaa tttctgcatt actactttca gaacaaacat
    18121 aactatatac agaacaacaa tttcccttta ctcccctact tattaatctc aatataccta
    18181 aatctataaa ttcatttaca agtctactta ttgtagatat agatacatca aggtcagatg
    18241 cagctttcct tactgtcatc ataaattgtc ctcttggcag agttcctttt aatctttgta
    18301 tattttcttt tctcattaca tagtgattaa accttattct atttacatct cttttttcat
    18361 taaaatataa tactgctgaa tttaattgat aaaaactatt actcatattc ccactcctcc
    18421 aatttaaata gataatttgt tgtaaagttt ttagattttt aaataactta ttaaatgatt
    18481 atttttatta aaaagtattc tttgatattt tcattttaaa gttttaagta taataagtta
    18541 atagtgtaga tttaactttt ttatttaaac aataaactta atattatttt taatataaaa
    18601 attttattaa atttagtatg taatttttta atcttatttt attccatcta tactcttatg
    18661 ctaagtaata actttgataa tatttcccta acttatgttt caactggttt atattgcaat
    18721 ttatttaatt acagcttatt tgcataataa ttcgtccata tcataagttg tgtatttata
    18781 taaatacaca attaaaaata aatcactatg gtatactaat tattacgaag actatactta
    18841 tatgcaaaga caaatataag gcatcttata t
//
LOCUS       DAYAOX010000060        18655 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.060, whole genome shotgun sequence.
ACCESSION   DAYAOX010000060 DAYAOX010000000
VERSION     DAYAOX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.060"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..321
                     /locus_tag="ACGI57_001657"
     CDS             <1..321
                     /locus_tag="ACGI57_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9716737.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="ACGI57_001658"
     CDS             337..1920
                     /locus_tag="ACGI57_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9716738.1"
                     /translation="MRILIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKNLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="ACGI57_001659"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="ACGI57_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFG9716739.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3475..4827
                     /locus_tag="ACGI57_001660"
     CDS             3475..4827
                     /locus_tag="ACGI57_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFG9716740.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5019..6128
                     /locus_tag="ACGI57_001661"
     CDS             5019..6128
                     /locus_tag="ACGI57_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9716741.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6411..7802
                     /gene="hydA"
                     /locus_tag="ACGI57_001662"
     CDS             6411..7802
                     /gene="hydA"
                     /locus_tag="ACGI57_001662"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HFG9716742.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7796..10357
                     /gene="xdh"
                     /locus_tag="ACGI57_001663"
     CDS             7796..10357
                     /gene="xdh"
                     /locus_tag="ACGI57_001663"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HFG9716743.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSIPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10509..11855
                     /gene="rpoN"
                     /locus_tag="ACGI57_001664"
     CDS             10509..11855
                     /gene="rpoN"
                     /locus_tag="ACGI57_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFG9716744.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12637..13659
                     /locus_tag="ACGI57_001665"
     CDS             12637..13659
                     /locus_tag="ACGI57_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFG9716745.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13719..14726
                     /gene="gap"
                     /locus_tag="ACGI57_001666"
     CDS             13719..14726
                     /gene="gap"
                     /locus_tag="ACGI57_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFG9716746.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14948..16150
                     /gene="pgk"
                     /locus_tag="ACGI57_001667"
     CDS             14948..16150
                     /gene="pgk"
                     /locus_tag="ACGI57_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFG9716747.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16176..16919
                     /gene="tpiA"
                     /locus_tag="ACGI57_001668"
     CDS             16176..16919
                     /gene="tpiA"
                     /locus_tag="ACGI57_001668"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFG9716748.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16931..18460
                     /gene="gpmI"
                     /locus_tag="ACGI57_001669"
     CDS             16931..18460
                     /gene="gpmI"
                     /locus_tag="ACGI57_001669"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFG9716749.1"
                     /translation="MKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTLI
                     NASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMDN
                     AKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALEY
                     AKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAIQ
                     NSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDGF
                     KREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAHV
                     TFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVLN
                     FANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPET
                     GKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTILDMMKLEKPEEMTGHSLIS
                     K"
BASE COUNT         7462 a         2159 c         3761 g         5273 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggctatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttgtga gaattttaat aaaacatggg
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtctag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgatggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagaact tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aagaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccgggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatgg ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aaatggaaag
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgattatgc aaaattctat
     3301 gaagcaggaa ccctgtgact ttagtcatgg gaggttcaga ttaatataca agtaagaaac
     3361 aagaatttag atgttaaaaa cagagcttaa acaaaaatag gaatttaaat gaagtatata
     3421 aataaaaaat atacggaata gaatattaaa ttaaatatag aagggagacc caaaatgtct
     3481 cgagataaaa gccctaaaat agctccagta gatgaaaggt tacctgtatc caaatcttgg
     3541 gtattttcac tacagcacgt tttagcaatg tgtgcaggag cagtagcagt tcctatgatg
     3601 ataggaagtg ctgcacgact agatcatgct agtattgttt ttttaataaa tgcttgcttg
     3661 tttatggcag gtataggaac actcatacaa agtgttggta taggaaatat tgtaggtgct
     3721 aaaataccag taatagaagg aactagtttt gccgctgtgg caggtatgac agcaatagcc
     3781 tcatcatata gtgattcaca agttgcaata cagacagtat ttggctctgt aatagtagct
     3841 ggattgtttt gtatattact tgctccagta tttgaaaaat taataagatt tttccctagt
     3901 gtagttactg gaacagttgt tatggtaata ggtatttcac tatttccagt tgggataaaa
     3961 tggataactg acaacaaatt ggaagcggtt gaaccaaact cattagcatt agctttagca
     4021 gtattggcaa taactttact ttcatttaaa ttcttaaaag gaatttggaa tagcacagct
     4081 atattggtag gtatagtact tggtacacta ctttccatgg cagttggaat ggcagatttt
     4141 agtgcagtaa atggagttgg ttggcttaac ttaaatgtac ctttaaaatt tggaatgcct
     4201 agatttgaac catctgcaat actatcattg tgtttgatta tgttggtact tatgacggaa
     4261 tcagttggta atatgatagc tattcatgaa atggtagaca aagaagtaac aggtaaaaat
     4321 attaaaagag gtctatttgg agatggaata tctacagcat tatctggaat attcaattca
     4381 tttccaataa ctccatttgg tcaaaataca ggaatagtag gtcttacagg tataaaatca
     4441 agatttatag cagtttacgc aggggttata ttgctagtac ttggattctt accaaaattt
     4501 gcagcaataa taggagcaat acctaagcct gttcttggag gcgtaggatt tgcaatgttt
     4561 ggaatggtaa tggttggagg agtaaaaact ttaagcaaag tgaaatttga tggaactaaa
     4621 aatggggtta tagtagcagt tagtgttggt ttgtctatga taccactaac aaatcctgat
     4681 ttctatcata atttcccaac ttgggtacaa acaatattcc atagtggaat aacaacaggt
     4741 agtttatcag cagtgctttt aaacatattc ttcaatgaac taggttctaa aaaagaaaaa
     4801 aataataaag ttgaagaagc aaaataaaaa taaaacaagt aaaaatctat attgacttaa
     4861 agtcattaag tattaataaa aataattcag agatattaat aattcaacaa aactatctta
     4921 tattttaaat aatataaaaa agtaaataaa taacaaatac aaataaaaga atacaatcta
     4981 aatattaaaa atatttaatt taagggagag ataataatat ggctaattta aaagttaatt
     5041 tatatggaat ggaattttta aatccaatta tgacagcggc aggacctgga gcaaaagatg
     5101 gtgctctctg tcaagaggca gcaaaaggtg gagcaggagc tatatgtaca aaaactatat
     5161 ctgtattacc agctgatgta ccaagaccat gtatggcaaa aactaatagt ggttttttaa
     5221 acacagaatt atggtcagag cttccaaaag agcaatggat agaggaagaa tacaaaaagg
     5281 taaaagaaac agggcttcca gtaatagtaa gtatgggata ttcagcagag caaatagctg
     5341 aggttgctcc tttagtaaag ccttatgcag atgcagttga attatctact cattatgtag
     5401 gtacagacgt tgcaccaata gtaaatgctt taaaagctgc aaaagctgtt ttagatgtac
     5461 cagtatttat gaaaatgagt cctcatactg acattcaaac tattgcaaaa gctgttgaag
     5521 aagcaggtgc agatggactt gtaatgataa actcatttgg accatgtatg gcaatagatg
     5581 taaatacagg ataccctata atgggaagta aagctggata cggatggtta tcaggtgcac
     5641 caataagacc tctagcaata agatgtatat atgaagcttc tcaagttgta aatataccaa
     5701 taatcggtgt tggtggaatc acttgtggaa aagacgtggc tgaaatgatg atggctggag
     5761 caagtgctgt tcaagtatgt acagaagcaa tccttaaagg gccttctatc tatggaaaaa
     5821 ttgctaaaga attaaatgaa tttttagatg agaatggata tgctgatgtt aatgaaataa
     5881 aaggactaac tcataagaaa atggcagcta gagggtttag aactcattca gtagctccag
     5941 tagtggatga taacaagtgt atcaaatgta agcaatgtgt aacaagctgt gtatacgaag
     6001 ctttagaagt tacagataag ttgaacatag atgcagacaa gtgttttgga tgtggattat
     6061 gtgtaactag atgtccaaaa ggagctttaa gcatctgcta caatcaagta gaagctatga
     6121 atgagtaata attatataga attttggtaa ttaacttaga gtaataggta ttaggataat
     6181 aaaatatatc agataaacaa acacacatta tgcattaata atacagtcta aagttaataa
     6241 ttttattata ttgatttaac taaatattag tttagctgaa tattaattta attaggtatt
     6301 ggtttaatct taatatagat ttactgcgta taagtgaact aactagatta ctttaagtta
     6361 attttttcat ataaaataaa ttgatacatt acaagtaagg tggagtctat atgggaattg
     6421 ttttaaaagg tgggaaagta gtaacaagtg aaaaaactta ctgtgcagat gtaagaatag
     6481 aagatgaaaa aatagtcgat ataggtcttg atatatcaaa agaaaatgat gaaatagttg
     6541 atgttagtga ttcttatgtt ataccaggag gtatagatac acacacacac tttgacttag
     6601 atacaggcgt tactaagaca gcagataact ttgagacagg aacaaaagct gctatagtag
     6661 gaggaactac tactatattg gattttgcta cacaagctaa aggatgttct ttgaaagacg
     6721 gattagaaga atggcatgaa aaagctaagg ataaatccta ttcagattat ggctttcata
     6781 tggctataac agattggaat gactcggttt gtaatgaaat ggaggatatg gtgaaagagg
     6841 gagtatcttc atttaaactg tatatggcgt ataaaggaag ccttcaagtt gatgatggtg
     6901 taatctttga agcattgaga aaagcagaag agataggagg gattattggt ttccattgtg
     6961 aaaatgggga cataatatgt gaacttgtgg ataaagcaaa gtcagaaaat aatttatctc
     7021 caaaatatca tcaattaact agaccagcta taatggaggc agaagctaca tcaagactta
     7081 tgaagatagc tgaagttgca aactctaaag tatatgtagt tcacttaagc tgtaaagaat
     7141 ccttagagga ggttttaaaa gctaagcaaa gaggtgtgga tgtaatagtt gaaacttgtc
     7201 ctcaatacct tctattaaat gatgaattat atgatttaga tggatttgaa agtgctaagt
     7261 atgtaatgtc accaccttta agaaataaag aaaataatga aatattatgg aatgctattt
     7321 tagataatca aatagacact ataggaacag accattgttc ttttaattat aagggacaga
     7381 aggaacttgg gataaacgat ttcagtaaga taccaaatgg ttctccaggt gtagaacata
     7441 ggatgagtct tttatacaca tatggagtct gtaaaaataa aatatctatc aacaagatgg
     7501 tagaagtaac atcaactaat gcagcaaagt tgtttggaat gtatcctaat aaaggaacta
     7561 tagaaatagg cagtgatgca gatatattag tcttaaatcc aaacaaaaaa tccaaaatag
     7621 aagcaaaaaa acaattacaa aatgtagatt atacacctta tgaagggtat gaagtggatt
     7681 gtcaaataga acatgtttat ttaagaggaa aagaagtagt tagagatgga gaattaattt
     7741 gtaatactcc aagtgggaga tatataaata ggcgtctaac aaagggggct agagaatggt
     7801 agaatttata ttaaatggag agaaagttgt tgcaaaagaa ggcaaaaaac ttttagactt
     7861 ccttagagaa gatatgaata taacttcagt taaaaatgga tgttcagaag gtgcttgtgg
     7921 aacatgtatg gttataatag atggtaaagc tactaaagca tgtgtattta aaactgataa
     7981 attagctggg aaaaacataa caactataga gggattatca gagagagaaa gagatgtttt
     8041 tgcatattct tttgctagtc aaggagctgt acaatgtgga ttttgtattc caggtatggt
     8101 aattagttca aaagcactta tagacgttaa taataatcct actgaagatg atataaagaa
     8161 agctttaaaa ggtaatatat gtagatgtac agggtatgta aaaataatta aagctgtcga
     8221 tttagcggca aagattttta gagaaaatat agatgtgcca aaagtaaact gtaagggtct
     8281 agtaggtgaa aatcttccta gagttgatgc agttggcaaa gcacttggat attctgaata
     8341 tgtagatgat atgagaattg agggtatgat ttatggttca gcagttagag ctaagtatcc
     8401 tagagcttta gttaaaaaaa tagatataga agaagcaaaa gcttatccag gagttgttgc
     8461 agttctaaca gctaaagata ttccaggaac tataaaggta gggcacttaa agaaggattg
     8521 ggatacttta ataccagaag gtgaaataac tagatactta ggagatggaa tagttttagt
     8581 agcagcagaa actagagaag cgttagaaga ggccaaaaag ctagttaaga tagattatga
     8641 ggaattaaca ccattaagta ctacagcaga tgcacttaga gaagatgctc caaagataca
     8701 tgagacaggg aatatacttg ttaaggaaca tttagtaaga ggaaacgcta aagaaaagat
     8761 agaaaattca aaatatgttg taactaaaag atatactaca cctgctacag aacacgcatt
     8821 tttagagcct gaatgtgcag tagctggtcc ttatgaagat gatggagtaa taatctattc
     8881 gacagaccaa ggggtgtttg ccactcaaca tgaatgtgca gatatgttag ggttaccata
     8941 tgagaaggta agagttataa acaaaatggt aggaggaggg tttggtggaa aagaagacat
     9001 gagtgttcaa caccatgcag ccttacttgc ataccataca aagagatttg taaaagtaca
     9061 gttaacaaga aaagaaagta ttattgttca tccaaaacga catgccacag aaatggaatt
     9121 tacgacagca tgtgatgaaa atggatactt aacgggtatg aaggcaacga taatatctga
     9181 cacaggtgca tatgcctctc ttggaggacc agtgctacaa agactgtgta ctcatgcagc
     9241 aggaccatac aattatcaag atattgatat aacaggaaca gcagtatata ctaataaccc
     9301 tcctggagga gcatttagag gatttggagt tacacagagt tgttttgcaa ctgaatgtaa
     9361 tctaaattta cttgctgaaa tggttggaat atctccttgg gagataagat ataaaaatgc
     9421 aataagacct gggcaagttt taccaaatgg tcaatttgca gatgaaggaa cagcaatggc
     9481 agagacacta gaagctgtaa aagaagagta tgaaaagcat ccacgtgcag gtatagcttg
     9541 tgctatgaaa aatagtgggg ttggagttgg tatacctgat gttggaagat gtagattggt
     9601 gataaaagat ggcaaagttc acataagaac aagtgcttct tgtataggtc aagggcttgg
     9661 tactgttatg actcaagtag tttgtgaaac tgctaaccta cttcctgaaa atatagttca
     9721 tgaagttcca gatactcatt taacaccaga ctctggtaat actacggctt caagacagac
     9781 agtatttacg ggagaagcta ctaaggttgc atcaaataag ttaaaagaag ctttagactc
     9841 tggcaaaact ttacttgatt tagagggaga agagttttat ggagaatatg aaagtattac
     9901 agataaaatg ggttgtgaca aagaaaatcc agtaagtcat gttgcttatg gatatgcaac
     9961 acaagttgta attctggatg aaaatggaaa actagaaaaa gtagtagcag cacatgatgt
    10021 tggaagagct ataaatccaa tcagtttaga aggtcaaata gaaggtggag tagttatggg
    10081 tcttggatat gctctaaccg aagattaccc agtagttaac tctattccta cagctaagtt
    10141 tggtacatta ggtttattta aatctacaga tataccagaa atacaagctt taatagttga
    10201 aaagaacaaa gatttattgg cttatggggc aaaaggtgta ggagaaattt gtacagttcc
    10261 tactgcacca gcagtacaaa atgcctatta tgtatttgat aaagaattta gaactgagtt
    10321 accactacaa aatactccat atagtaaaaa aaagtaacaa aaaaaagaaa tattaaataa
    10381 aaaaatagat gaaattgtaa ataaaaaaaa cccttttgca taaggtatat tttaaatgat
    10441 agaatataca tatacaggag ggtttttctt ttataaaata agtttaataa aattgagagg
    10501 tgagcagaat gaattttaat aatagtctag aattaactca gtctcagaag ctaataatga
    10561 ctacacaact taagcaatca ttatcaatat taaatatgag taagttagag ttagaagaag
    10621 agataaaaag agaagcagag aataacccat tagttgaagt cgagaaaagt ggggagataa
    10681 attgggaaga gtatataaaa gacatggata ggtcaagacg tttagataga acagagataa
    10741 gctataatcc tgataatgaa gttaatctag aaaatttagt taaatattcg tcaaatttat
    10801 atgaagattt aaaatttcag ataagcctgt ataaacttac agacaaagaa ttggaagtct
    10861 gtgagtatat aattgatagt ttagatgagg atggatattt aagaactgag gaaaaagtaa
    10921 ttgttgatac cttaaagata gatgaagaac tttttgaaaa atgtttaatt agtgttcagc
    10981 agttggaacc tagtggagta ggggcaagaa gtctttcaga atgtctgatg atacaaatgg
    11041 ctagtttagg aatatataat gaaattttag aggaaatcgt tacaaaagac ttaaatttaa
    11101 ttgcaaataa taaatacaaa gagataagta aaaagtacaa tatgtcatta caaaaatgtg
    11161 ttgaactaat aaatataatt aagacattag accccaaacc aggaagaact tgttcagtag
    11221 agaaaagtgt atacattcaa cctgatgtaa cagtagaaaa aattgatgat gaatttattg
    11281 tatatttaaa tgagaaagat tcttatcaaa taagaatcaa tagttattat aaagacatat
    11341 taaaaaattc acagtctgat gaaagtgcaa aagaatttat aaaagaaaga ctaaattcag
    11401 caacaggact tatgaaaaat atagagagta ggaaaactac agtacttaag attgcagaag
    11461 agatagtaaa agctcaagat gaatttttga gaaaagggac aaaatatata aaacctttga
    11521 aaatgaaaga catagcagaa aaattagaat ttcatgaatc tactataagt agaggtgtaa
    11581 atggtaaata tatgctaaca ccttttggcg tatatgaatt taggtacttt ttcagtagcg
    11641 ccattgagac tgagaataat gaaatggcat ctagtacaag cataaaaaaa attataaagg
    11701 aaacaataaa agacgaaaat aaaaagaaac ctttaagtga tgaccatata tctaagttat
    11761 taaaagaaaa aggaatcaat gttgcaagaa ggacagttgc caaatatcgt gaagaattag
    11821 gaatcccatc ttcaagtaag agaaaagaat actaataagt aaaagacaaa tatcagtatg
    11881 gtaaggggaa aaggctaatt aagagatgaa tgtagattgg gctaaaaatt ttataaagta
    11941 aaataatata atatgaaaaa gtattaaaat gctataaggt attaaaaaac tggggaacat
    12001 taaaacatta agatagcaaa gagcataaaa aactaaagaa gattaaaaca ttaaagaaca
    12061 ttagagcttt gaggaatatt agaactttaa agaatattaa aatttataaa atataaaaat
    12121 actaaaaaga attgaaaaac tggggaacat taaaatattg taaaatgcta aaaaactaga
    12181 aaacataatt aaataagtgc ttataaagaa tgattaaatt aaataagtgt ttgtaaagaa
    12241 taattagata ttagaaaaag aagaatacct agataaataa actattatta aatttaataa
    12301 tcacaaaagg aaaacacgta gggtataaat aaaaatctta actagataaa agtaaactta
    12361 gttgtagata aaaataacta gaaataaaat tatttatagt aataaatatt agaatgaaaa
    12421 tataaaaaaa tatatataaa aagccttata taaaaagaaa aaatactgtg atttgaaaat
    12481 ggtggagaaa tattttataa aataaaaaaa taaaaaagtt tttgaaaaac tattgaaatc
    12541 tatacgcaaa tcctataaaa taaaaatatc ttgtgggact taaataaaca ctagggacaa
    12601 aaaaagtccc gaaaggaaag caataaggag tatgttatga agaacttatt agaaattcaa
    12661 aagaaattga ttccacaggc aattgaactt atggaaagaa gatactctat tttaagacaa
    12721 atatctttaa gtgaaccaat aggtaggaga acattatcaa atgtattaga gattagtgaa
    12781 agaataatca ggtcagaaac tgagttttta aaagaacaag gacttataga cgtagctgta
    12841 tctgggatga ctatcactaa agaaggttct gatttgttag ataaacttaa ggacatcatg
    12901 agtgacatca tgggattgtc taagttacaa gatagagtca gagaaaagct tggaataaaa
    12961 agggtaatat tagttcctgg aagctgtgat gaaaatggaa gtttactaaa agatgtaact
    13021 agatatggat gcgaatattt cttaggcatt ttaaaagatg gagatatcgt ttcaataaca
    13081 ggaggaagca ctatgttgga gttttcaaac gttattaaaa ctgataagag atatccaaac
    13141 tctacaatag ttccagctag aggaagtatg ggaactgatg tagagatgca atctaacaat
    13201 atagttgctt caataagtaa gaaattacat tctaattgca aacttttaca tataccagat
    13261 gagttggatg aaagtgcaat gaaaactctt agccaagtgc cagagattaa gacaactttg
    13321 gagtatatta aaagaacaga cattttagtt tttagtatag gtagagcaga tgtcatggca
    13381 aagagaagaa aacttccaga agacaaggtt gatgagatat tatctaaaaa tgcagttgga
    13441 gaagcatttg gatattattt caacaaagat ggagaaatag tttacaaact gaacactgtt
    13501 ggaattgatt tagaaacagt taaacaagta aaagaaacta ttgcaatctt tgcagggact
    13561 gaaaaagtag aagccttatt agctatatca aatatgcata aaaacatggt tcttgtaaca
    13621 gatgaagaaa gtgcttataa gatattgtca ttattataaa agtcaagacc tagcacagag
    13681 ctagaaaaca tacatttact ttataggagg tagcgaaaat ggttaaagta gctataaatg
    13741 gatttggaag aataggaaga ttagcattaa gaaaaatgat ggagcaacaa gataagttcg
    13801 aagttgttgc aataaacgac ttaacagacg ctaaaatgtt agctcattta tttaaatatg
    13861 atacagctca aggaagattt aacggagaaa tagaagttaa agaaggtgct tttgtagtaa
    13921 acggaaaaga aataaaagta acagcagaaa gaaatcctgc tgacctacca tgggctgaat
    13981 taggagtaga tatagtatta gaatgtacag gattctttac atctaaagat aaagctgaag
    14041 ctcatataca agcaggggct aagaaagttg ttatatctgc accagctact ggagatttaa
    14101 aaacaatagt attcaacaca aacagtgaca tattagatgg ttctgaaaca gttatatcag
    14161 gagcttcttg tactactaac tgtttagctc caatggctaa agtattaaat gataaatacg
    14221 gtatagaaaa aggcttaatg actactatac atgcatacac taatgaccaa aatactttag
    14281 atggtcctca tccaaaaggt gatttaagaa gagctagagc tgctgctgga aacatagttc
    14341 caaacactac aggagctgca aaagcaatag gcttagtaat accttcatta aaaggaaaat
    14401 tagatggggc agcacaaaga gtaccagttg taactggttc aataactgaa ttagtttgta
    14461 cattaggaaa aaatgttaca gttgaagaaa taaatgcagc tatgaaagaa gcttcaaacg
    14521 aatctttcgg atatactgaa gaaatgttag tatcttctga cataatcgga ataagctacg
    14581 gttcattatt tgacgctact caaacaaaag ttatggaagt agatggaaaa caattagtta
    14641 aagttgtttc ttggtatgat aatgaaatgt cttatacttc tcaattaata agaactttag
    14701 gatactttgc acaattagca aaatagtcag ttttgagaaa atagaaaagt agattgacaa
    14761 agtccgagtt tgtagtttaa tactacggac tcaggacttt ttttaagaaa aaatatataa
    14821 aatacaccta aataatgtta catactatat ataaaaatat gataatatga tatatttaat
    14881 agggtaaaag tatgtactat attatacttg gcaataaaaa attactttat taaaaggaga
    14941 gatatttatg tcaatgctta acaaaaaaac tatagaagat attgatgtgt gcggaaaaaa
    15001 agtattagta agatgtgact ttaatgtgcc attacaagat ggtgtaatta ctgatgaaaa
    15061 tagattgaac ggagctttac caactattca atatttaatt tctaaagggg caaaagttat
    15121 attatgctct cacttaggaa agccaaaagg agaagctaaa ccagaattat ctttagcacc
    15181 agtagcaaag agattatctg agatgttagg aaaagaagtt gtatttgcag cagacgataa
    15241 tgttgtagga gaaaatgcaa agaaagcaac tgaaaaaatg gaaaacggag atgtagtatt
    15301 attagaaaat actagatata gaaaagaaga aactaaaaac gaagagaatt tctctaaaga
    15361 actagcatca cttgctgaaa tatttgtaaa tgatgcattt ggaactgctc atagagcaca
    15421 ttgctcaaca gttggtgcag gcgagttttt acaagagaga gtttgtggat acttaattca
    15481 aaaagaatta aaattcttag gagaagctgt tgcaaaccct gtaagaccat ttacagctat
    15541 acttggagga gctaaagttt ctgataaatt agctgttata aacgaattat tagaaaaagt
    15601 tgataatcta ataataggtg gaggaatggc ttatacattc ttaaaagctc agggttatga
    15661 agtaggaact tctttacttg aaatagataa agttgaatat gctaaagaaa tgatggaaaa
    15721 agctaagaat aagggtgtta acttattatt accagtagac gttgttatgg cagaccactt
    15781 tgcaccagat gctactccaa tagtaacaga agatgctaat gtaaaagaag attacatggg
    15841 actagatatg ggacctaaga ctatagcaaa ctttgtaaaa actataaaag aatcaaaaac
    15901 tgtagtatgg aatgggccaa tgggagtatt tgaatttgaa aactttgcaa atggtacatt
    15961 atcagtagct agagctatgg cagagttgac tgatgcaact actgtaattg gcggtggaga
    16021 tagtgcagca gcagttaatc aattaggatt tggtgacaag atgactcacg tatcaacagg
    16081 tggaggagca tcactagaat ttttagaagg taaagaatta ccaggtattg cagcattaga
    16141 taataaataa actaatatat agaggagatt gtaaaatgag aaaacctata attgcaggaa
    16201 actggaaaat gcataaaaca ataaaagaag ctttagaatt tgtaaatgaa gttaaagata
    16261 aagtaaattc tgataaagta gaagctgtta tatgtgcacc atttacttta ttaaaggatt
    16321 taaaagaagc tactaagggt acaaatataa agataggtgc tcaaaatatg cactttgaag
    16381 aaaaaggagc atttacagga gaagtttcac ctcttatgtt aaaagaaata gatatggact
    16441 atgttgtaat aggacattca gaaagaagac aatacttcaa tgaaactgat gaaactgtaa
    16501 acaagaaagt tttaaaagca ttagaagtag gaatagaccc aatattatgt gttggagaaa
    16561 ctttagaaca aagagaagct ggaaaaacta aagatgtttg taaagttcaa gttgaaaaag
    16621 ctttagaaaa tgttcttaaa gatgatttag caaaagtagt tgttgcatat gagccaatct
    16681 gggctattgg aactggtaaa actgcaactg ctgaagatgc taatgacgtt atatcttata
    16741 taagagaagt tataaaagga ttatatggag aattagctaa tgaagttaga atacaatacg
    16801 gtggaagtgt taaaccttca aatgtagccg aaataatggg tcaaagtgat atagatggag
    16861 ctttagtagg tggagctagt ttagctagta atgattacct agaccttgtt aatttctaag
    16921 gagtgaaatg atgaaaaaac cagttgcttt aattattatg gacgggtttg gatacaataa
    16981 ggatgttaag ggaaatgcaa ttgctgagtc caaaactcct aatttagata gaataaaaaa
    17041 agagtatcca aacacattga taaatgctag tggtcttgat gttggtctac ctgatggtca
    17101 aatgggaaat tcagaagttg gacacactaa cataggtgca ggtagaatag tatatcaaga
    17161 tttaactaga ataacaaaat ctataaaaga tggtgacttt tttactaata aagttttatg
    17221 tgaagcaatg gataatgcaa aagaaaactc tcttcatgta atgggacttt tgtctgatgg
    17281 aggagtacat tcacatatag accatcttaa ggctataatc aaaatggcaa aagataaagg
    17341 cgtacaaaag gtgtatgttc acgcatttac tgatggtaga gatactgacc ctcaaagtgc
    17401 attagaatat gccaaagaag tacaagctag tatggatgaa attggagtag gtgaatttgc
    17461 tacagtttct ggtagatact acgctatgga tagagataag agatgggaaa gagtagaact
    17521 tgcatacaat gccatggtta gaggtattgg tgaaaaagca aattctattg aggaagctat
    17581 tcaaaactct tatgatgatg gaaaaaatga tgagtttata atgccaacag ttattatgaa
    17641 agatgataaa ccagttggaa gtataaagga aaatgattca attatattct tcaactttag
    17701 accagataga gctagacaaa taactagagc tttagtttgt gaagagtttg atggatttaa
    17761 aagagaagat attaaaaact tctttgtatg cttaacagag tacgatataa ctatagagaa
    17821 tgtgcatata gcatttggac ctcaatcttt agccaacact ctaggtgagt atctagctaa
    17881 aaatggaaaa actcaactta gagctgctga aactgaaaag tatgcacatg taactttctt
    17941 cttcaatggt ggagtagaag agccaaataa aggtgaagaa agactattga taccttctcc
    18001 aaaggttgca acttatgatt taaaaccaga aatgtcagca tatgaattga cagataaagc
    18061 tttagataaa ttaggagaag ataagtttga ctttatagta ttaaactttg caaatcctga
    18121 tatggtaggt cacacaggaa gtatagaagc ggctataaag gctgttgaaa ctgtagatac
    18181 ttgtgtagga agattgatag ataaaatagt agagttaggt ggaagtgcaa taataactgc
    18241 tgaccacgga aatgctgaat atatgttaga cccagaaaca ggaaaaactg taacggctca
    18301 ctctattaat ccagtaccat ttattgtagt tggtcaagaa tatgaaagtg caaaattatt
    18361 ggatggagga agattatctg atatagcacc tactatttta gatatgatga agttagaaaa
    18421 gccagaagaa atgactggtc attcacttat ttcaaaatag tcgattctga taagtagata
    18481 ataatatata aactacaata taaatttaaa aataatgcag tttaggttta ttttgtaaag
    18541 tttagacttt atgacataag gtttaaattt tgtaagttct ggatattgta aggtgtagat
    18601 gaagatatat tttataaaat ttacaagata gacaatttat aaaataaaaa attac
//
LOCUS       DAYAOX010000061        18462 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.061, whole genome shotgun sequence.
ACCESSION   DAYAOX010000061 DAYAOX010000000
VERSION     DAYAOX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.061"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..360)
                     /locus_tag="ACGI57_001670"
     CDS             complement(<1..360)
                     /locus_tag="ACGI57_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9716750.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKE"
     gene            927..1289
                     /locus_tag="ACGI57_001671"
     CDS             927..1289
                     /locus_tag="ACGI57_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716751.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1273..1998
                     /locus_tag="ACGI57_001672"
     CDS             1273..1998
                     /locus_tag="ACGI57_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716752.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQN
                     IIENIKKNFDEKNLVQVYKYRGV"
     gene            2018..2437
                     /locus_tag="ACGI57_001673"
     CDS             2018..2437
                     /locus_tag="ACGI57_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716753.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2427..2951
                     /locus_tag="ACGI57_001674"
     CDS             2427..2951
                     /locus_tag="ACGI57_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716754.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            3263..3820
                     /gene="efp"
                     /locus_tag="ACGI57_001675"
     CDS             3263..3820
                     /gene="efp"
                     /locus_tag="ACGI57_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFG9716755.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            3912..4367
                     /locus_tag="ACGI57_001676"
     CDS             3912..4367
                     /locus_tag="ACGI57_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1247 N-terminal domain-containing protein"
                     /protein_id="HFG9716756.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            4530..5240
                     /gene="rnc"
                     /locus_tag="ACGI57_001677"
     CDS             4530..5240
                     /gene="rnc"
                     /locus_tag="ACGI57_001677"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFG9716757.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5294..6364
                     /locus_tag="ACGI57_001678"
     CDS             5294..6364
                     /locus_tag="ACGI57_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006448 - regulation of translational
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongator complex protein 3"
                     /protein_id="HFG9716758.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6392..9946
                     /gene="smc"
                     /locus_tag="ACGI57_001679"
     CDS             6392..9946
                     /gene="smc"
                     /locus_tag="ACGI57_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFG9716759.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKFAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            9973..11253
                     /gene="ftsY"
                     /locus_tag="ACGI57_001680"
     CDS             9973..11253
                     /gene="ftsY"
                     /locus_tag="ACGI57_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFG9716760.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11356..11724
                     /gene="ylxM"
                     /locus_tag="ACGI57_001681"
     CDS             11356..11724
                     /gene="ylxM"
                     /locus_tag="ACGI57_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HFG9716761.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            11721..13073
                     /gene="ffh"
                     /locus_tag="ACGI57_001682"
     CDS             11721..13073
                     /gene="ffh"
                     /locus_tag="ACGI57_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFG9716762.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13111..13383
                     /gene="rpsP"
                     /locus_tag="ACGI57_001683"
     CDS             13111..13383
                     /gene="rpsP"
                     /locus_tag="ACGI57_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFG9716763.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13448..13675
                     /locus_tag="ACGI57_001684"
     CDS             13448..13675
                     /locus_tag="ACGI57_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFG9716764.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            13783..14298
                     /gene="rimM"
                     /locus_tag="ACGI57_001685"
     CDS             13783..14298
                     /gene="rimM"
                     /locus_tag="ACGI57_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFG9716765.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14307..15002
                     /gene="trmD"
                     /locus_tag="ACGI57_001686"
     CDS             14307..15002
                     /gene="trmD"
                     /locus_tag="ACGI57_001686"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFG9716766.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15187..15537
                     /gene="rplS"
                     /locus_tag="ACGI57_001687"
     CDS             15187..15537
                     /gene="rplS"
                     /locus_tag="ACGI57_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFG9716767.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            15858..16754
                     /gene="ylqF"
                     /locus_tag="ACGI57_001688"
     CDS             15858..16754
                     /gene="ylqF"
                     /locus_tag="ACGI57_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFG9716768.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            17143..18435
                     /gene="brnQ"
                     /locus_tag="ACGI57_001689"
     CDS             17143..18435
                     /gene="brnQ"
                     /locus_tag="ACGI57_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFG9716769.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLFVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7805 a         1728 c         3427 g         5502 t
ORIGIN      
        1 ttctttatct gttccgatag ggtcttgtag agatacttga gttttatttt ttttgttaga
       61 tctaatattc attaaaatct cattttcaat acatctagaa gcatatgttg ctaatcgagt
      121 tcctttatct atgttataag tttctatagc tttaattaat cctattgtac ctatggatat
      181 taaatcatcc tgatcttccg ttgaattatt atatttcttt gctatataag ctactaatct
      241 catgtttctt tcaattaatg tatcttttgc acttttatcg ttttctattt taaatttagt
      301 taaatactca atctcttctt caggggttaa gggtttttca aaagatttaa gagctgccaa
      361 taggtcaccc ccttttcaac ggtcacttta aattatatga aggagactta tccaagttga
      421 ctgttatcta ataaaataat atgcatattt ttagaattta aaacatatat ttgcaaactt
      481 ttgaatggaa tatgacataa agagtgggaa tttaaattta agtttttgtt gtatatatat
      541 gaaataaggc gaagaaatta ttgatttata aagtataaat tggaattgag gtgtcaataa
      601 atataaaagg aaatagtatt tatatatgtt ttggtgttga agtaaaagat taatgcaaag
      661 cattagagtc tataaaaata tgaatatagg aggtgtaatt ttacaatact taaaaaagta
      721 aatgtaatta attgtttgta taaagttgat tacaagcaaa agtatcagtc atagttaata
      781 ctgttaaggg tgaaggtggt gtgttaattt atatatgtat gggaagaaat tttgattaca
      841 atgaaaaaac ttacaaaaga tatttatgaa aaaacttata gttaaatgtt tatatataaa
      901 atattattta ggggttgttt ttattattga aaataagaaa gagtggtttt atatcaattg
      961 aatgtataat aagtattgct atattatatg tggctgttta tttagtttct acatcattgt
     1021 ataattgtta tagttttatc agtagaaata tatctgacag agaaatgtta agtacagcaa
     1081 aaaaatatat agaagatgag aagtatagaa tacaaaatag taagtatgag ttaattgaag
     1141 ataagataga aaaaaattac ataaatggat atgaaattaa cagtaaaata gagcaaattt
     1201 tagattatta tcaatgctat gaaataaata tagagataaa aaatgaattt aaaaaactga
     1261 ggtttaatag ctatgttact agaaaataaa ggctctatat taataataac attgataata
     1321 ttttctatta taagtactgt gtgcattatg tgtataggtt taatttatac acataataat
     1381 atgtttgagt taggatacaa ggacattcaa ctaaaagaaa tttctttaag tggaatagaa
     1441 atatcagtaa gtaatgtact ttcttgtgtt gaggaagcta ttgatgtatc taataatgaa
     1501 gacgaattta gagaatattt cttaggaaat aatatgattg aatgtataaa aaaaataaaa
     1561 gatacatctt atagtagttt agaagaactt tctttaaaag tagaaaataa tactatttat
     1621 gacagaggag atttttttga atttgatata gaaagtaatg ttagagaaaa agaattttta
     1681 aaagcaataa aagtaagagt gaaaatcaaa aatccatgga taggaattga cattgaagat
     1741 tatatggata aatctaataa caataaaatt gatgacaaaa cattcaataa ttataaagca
     1801 tctgaaaata atgaaattct aaatcaattt aaaaatgaaa gtgaaaatat aaggaatcaa
     1861 gaggttggtg gatttacaga agaagatgaa gaagatgaag aatatgaaga agaacttaaa
     1921 caaaacataa ttgaaaatat aaagaagaat tttgatgaaa aaaacctagt acaggtatat
     1981 aagtataggg gtgtataact ttgaaaaaag aaggaatgtg actgtaatag aattaatagt
     2041 atctatcagt ataatactct tgatagttac attatctttt ccaaaagata atcttgaaaa
     2101 tcataaaata aatttatttg cacgtcaact ttgttcagat ataaggtatg ttaggcgaat
     2161 taatatgttt agcaactaca atacatatat atattatata aatcaaggta aacacgaagg
     2221 ttatgtattg agaaaagatg gtaaaaacat taaatcaata atgctaccta aaaatgcaaa
     2281 aatagctagg agtattgaaa taataaagtt taaatctgat gggtcgcctc aaaaggcgtc
     2341 tacaatagag atatataata atagattaag aaaaactata acaataacac ctgtaagtgg
     2401 aagagtactt ctgaaagagg gaaaatatga aacataaata tggttacttg cttttggaaa
     2461 gtgtcgtatc attgtcttca atggtgataa ttatactagt tttatattct atatttttgt
     2521 ctacaataaa cttaaagtta aaagtagaag ataagataga actacaacaa caaagtcttg
     2581 aaatcataaa atctatggaa ggaattatta gcaattctat gggaataatg aatgtatcta
     2641 attatgaaga aacatttaaa aagacaacat ctataaaatg tagatattta gatgaaaata
     2701 ataatgaaga aggcataagt aataaagaaa ttatattgaa tgaaagacga aataagttat
     2761 ttgtaaattc tttaaatggc gaaagtagtc aagcaggtgg ttatgaaatt ggagattatg
     2821 tagatgagat gtatgtatca attactaata atggtcaata tgttaatatt aaattgaaat
     2881 tatcaaaacg tagtcaaaaa tatgagactg atttcaaaat aaaagtatgg aattttagtg
     2941 aaagtatata gaaatacata aatttaagag aaagaagtga catcaataag tcatataaaa
     3001 tataattatt gagaactata taactaaata atatcaatat gtaaagtgta tagattaaga
     3061 atatagagga taataataaa aatatagaat aagtaatttg ccaaatactt gtttatattg
     3121 tataacttat gatattatga tattagtaaa aatttgtaat cctgacttga cagaattcaa
     3181 gtaagagata taattttaaa ggcatagtaa tgtaaaattt actaaaaaat aagaaattta
     3241 ttttaatatg gaggaacaaa aaatggtatc agcaggtgat tttagaaaag gtgttacatt
     3301 tgaaaaagat ggacaaccat gtttagtagt tgattttcaa cacgttaagc caggtaaagg
     3361 agctgctttc gttagaacta aatacaaaaa cttaaaaaca ggagctataa gagaagaaag
     3421 tttcaatcca agtgaaaaat tccctaaagc tgttatagat acaagacaaa tgcaatatct
     3481 atacaatgat ggtgagttat attattttat ggatcaagaa aattttgaac aaataccatt
     3541 aaactatgag caagttgaag atgcaattaa gttcttaaaa gaaaatgaag ttgctacaat
     3601 aagattttac caaggacaac cattccaagt ggaagcgcca aactttgcag agctagaagt
     3661 tacagataca gagccaggta taaaaggtga tacagcaagt aatgtaacta aagcagctac
     3721 agttgaaact ggagcagttg ttcaagttcc attatttata aatactggag ataaagtaaa
     3781 gatagatact agaacaggtg aatatttatc aagagtataa aaatttatgt atataaaagc
     3841 ttatatgtct taataatata agatatatga gcttttagtt tatatataaa aaagaaagcg
     3901 aggggtgtta catgaaacat ttatatgaag aagttgcata cttaaaaggc ctagcagaag
     3961 gactagaaat aagtgctgaa agtaaagaag ggaaaatgat acataaaata gttgatgcat
     4021 tagaagtatt tgcagatgct attgtaacat tggatgaaga acaagaagaa cttcaagatt
     4081 ttgttgaatc tatagatgaa gatttagcag atttagaaga agatattgct gacatggaag
     4141 aagatcttta tgaagaagat gacgatgagg atgatgagga cttcagctat atagaaatgg
     4201 aatgcccaaa ttgtggagag ttagtagaaa tagacgaaga tttgttatat gatgatgaag
     4261 tagatgttgt ttgtcctgat tgtaaggctg tgatattatc atcagaagat gattgtgatg
     4321 atgattgtac atgtggtggt tgttcaagtt gtgatgatag agagtaaaaa ttcataagaa
     4381 ttaaaactgc ctatgatgcc tatgagatat tttgcttcat aggtagtttt atttttatga
     4441 ggaagggtat tatgtatttt ggttttgcaa tacttggaca aaaatagtac actagaattt
     4501 atagaatagg attttggagg gatgtaaata tgaaaataag taaaaaatta ctagataata
     4561 tacaaagatt tgaaaatgtt ataaattata aatttaaaaa taaagaatat atactagaag
     4621 ctcttactca tagttcatat tctaatgaaa ataaaaaata taattttaac gaaagattgg
     4681 aatttttagg ggattcggtt ttaggcatag tgataagtga ttatttattt aatgaagaag
     4741 ctaatctgcc cgaaggggaa ctaactaaac ttagagcaaa tatagtttgt gaagattcat
     4801 taagtgaagt agctaatgat ataaatttag gtattcacat gttattaggt agaggagaag
     4861 aagcaacagg aggaagacat aggacttcaa tattagcaga cgcatttgaa gctgtaatag
     4921 ctgctatata tcttgatgga ggttttgaaa gtgcaagaca gtttatactt catcatatgg
     4981 aaaatataat ttatgattca agaaaaggaa atatatttag agactacaaa acgcatcttc
     5041 aagaggtgtt acaagggaat ggagaaaata atatctggta caggcttata gaagaaaaag
     5101 gaccagacca taataaaaga tttgtaatgg aagttggaat taatgacgat gtacttggta
     5161 taggagaagg caagagtaaa aaagaagctg aacagttagc tgctaaaata gctttaaaga
     5221 aaaaattatg ggaaaaataa atataatatg taaaaatata aaaagaaata tttatattag
     5281 aaggatgttg tagatgaaaa agagaattat acctatattt gttcctcaca aaggatgtcc
     5341 tcatgattgc atattttgta atcaaaaaaa aataacaggt gtgagtactg atgttactag
     5401 tgaagatgca agaaatataa ttgaggaatg tttagagact atagataaag atgcagatgt
     5461 agaaatagca ttttttggtg gtagctttac tgctatagat attgatatac aaaaaagttt
     5521 attatcagtt gctaaagaat atgtagaaaa aggtcttgta aaagatatta gaatgtctac
     5581 aagaccagat tgtattgatg aagatatatt aagtatgtta aaagaatata agacaagtat
     5641 aattgaacta ggagtacaat ccttagatga aaaagtatta ttagatagta taagaggtca
     5701 tcaatctgaa attgtataca agagttcaaa gatgataaaa aatagtggaa tcaaacttgg
     5761 attacagatg atgattggtc ttccagctga cacagaagaa aagtgtatat ttacagcaaa
     5821 aaaatttgta gagctaaaac cagattgtgt tagagtctat ccaactttgg tagtaaaaga
     5881 tacaggtctt gaaaagttaa tggaacaaaa taaatataat ccatttactc ttgaagagag
     5941 tgtaaatata gtaaaaaaag ttcttgtact gttttatgtt aataatatca atgtaataag
     6001 agttgggctt caagctactg atgatataca aattggaaaa gctgttttag caggaccata
     6061 tcatcctgca tttagagagt tggtagaagc agatatgata aaagattatt tagaatttgt
     6121 tattttgcaa aataaaaata taaagcaaat gttggtcaaa tctaataaaa agaatatttc
     6181 taaaatcata ggaaataaaa aaacaaatgt taaatatatg gaagaaaaat ttggtgtgct
     6241 tttaaaaaca caagaatcag atttagatat aaatcaatta gaaattgtgt tagatgggaa
     6301 aagtttaatt agtgctaata tgagagacat tcatagaaaa ttgtatgaca tttatgacct
     6361 ttagttttaa taatgaaggg agagaaaaga tttgtattta aaaagactag agttaaaggg
     6421 atttaaatcc tttccagtaa aaacagatat tatttttaaa gaaggaataa cagccatagt
     6481 aggaccaaat gggagtggaa aaagtaatat atctgatgct gtaagatggg tattaggaga
     6541 acagagtatt aagagtctta gaggtgataa actcgaagat gtaatatttg caggtacaga
     6601 tacaaaaaaa cctatgaatt attgtgaagt ggctcttact attgataatt ctgaaaatca
     6661 attggaatta gattttacag aagtaactat aagaagaagg gcttatagaa atggtgaaag
     6721 tgaatttttt ctaaataata aaagttgtag attaaaagat ataaaagaag tctttttaga
     6781 tactggtatt ggaaaagatg gttattcaat catagaacaa ggaaaagtag atgaaatatt
     6841 aagtaataat cctttaagca gaagaaaagt ttttgatgaa gcttgtggca tatcaaaata
     6901 tagatataaa aaacaggaag cagaaaggaa tttaagtaat acaaaggaaa atttagaaag
     6961 aatagatgat gtatatatag aaattgaaaa tcaattaaag cctcttttta atcaacagac
     7021 aaaggcaaaa aagtacttgg aaataagtga aaaattaaag acacttgaag taaatagctt
     7081 tataagagaa attgaaggaa tagaaaaaga attaagtgag gttaatgaac atcgtaaggt
     7141 tattgaaaag gagctcaatg aaaaagaaga acaaaaaaat gttgtagaga aaaagcaaga
     7201 agatattaat aaagaggtag aggtattaca agatgtaatt gaaaaatctg tagattatat
     7261 aaattcaatt aaaggagtta tatcaaaaaa agagtcccaa ataaatttaa ttaaggaaag
     7321 aataagaaat tttacgaatg aaattagtag aaaaaattta gaaataaagg atataaaaga
     7381 gaagctaaat gaaaataagc aatacataaa agaattagaa agcaataaat tatcaggtag
     7441 tgaagaacta tctaccttgc aggaaaatat aaaagtattg gaaggaagta aagataaaca
     7501 aaaaattaag ttagaatccc taaataatga gatagaatta ttgaaagaaa gtataattga
     7561 tatattaaat aagaaacaag agtttagtaa taagctgtct actttaaacg caaataaaga
     7621 aaatatgaat atcagagatg aaaatataaa ttcagagata acagagttaa acaaaaatat
     7681 tgaaataaaa tcatctgaat tagatactat aaataaagaa tttaatatgc aaaatgaaaa
     7741 tttaaagaat gtaaataata gacataaaga attatcaatt aatttgcagg attcaattag
     7801 tgaacacaat aaactagaag atgaaataca gaaaagtaaa tataatttaa atggatataa
     7861 ttcaaagtta aatgtctata tagatatgga aaatcattat gaaggattta atagaggagt
     7921 aaaagaagta ctaaaaaata aaaatttaaa aggagttcat ggagcattag gacagattat
     7981 aaatgtacct gaaaagtatg agaaatctat agaagcagct cttggtgctt atatgcaaaa
     8041 tataataact gataatgagt ttagtgctaa gtttgcaata aactatctaa aacagaataa
     8101 tttaggtaga gtaacatttc ttccattaaa tataattaaa tcaaataaaa taagtctagg
     8161 aaatttaaaa gcaaatacta agttcatagg aatagcaagt gatttaatta catttgatga
     8221 aaagtataga aatataattg aaaatatact tggaagaaca atacttataa ataatataga
     8281 tgagggcata aaatttgcaa aagaaactgg tcataggttt aaaatagtaa ctttagatgg
     8341 tgaaatctta aatccaggag gttctttgac tgggggaagt ctaaaaacta atgggaatat
     8401 actatcgaga aaaagataca ttaatgaata tacagaaaag ataagtaata tcaagaatga
     8461 aatttcacat ctagaattaa aaagagaatc tttagacaaa gatgtgaaaa atatcaaaaa
     8521 tgaaattgat tcacatgaaa gtaaaataaa agatttagaa aaaagtataa taataaaaag
     8581 tacaagtata aagaatgttg agtctgaaat tgaatcgtta aaaggtagta ttactaaatt
     8641 agaaaatgag aagaatgatt taaattctaa cctaaattat acacttgaaa aaagtgatga
     8701 tgttagaaaa gatatggaag agttagatga tttatacaat aaaaataaag aaaaaattga
     8761 tgcattgaat gaggaaataa aaagatacaa tgatttatat gataaagaaa aatctgaatt
     8821 tgatgaattg aacttaagtt tagtgaagaa aacagaagtt tacaatagta tagttagaga
     8881 tataaaaaga atatctggtg aaaattgtga gctagaagaa aaaaataaac aattagaaga
     8941 gtcattaaat tatgaagaac atgaaataat taaattacaa gattctatac tcactgaaga
     9001 aaaagaaaaa gaaaatttaa caaaacaact aggggatagt aatagaaatc ttgaaactag
     9061 aaaaatagca aaagatgatt taaaaaatag ttttgatgaa attaataaag aattaaaaac
     9121 tatagataga cagcatatag aacttaaaga aagtttattt aaagttggtg gaagattaga
     9181 aagattaaaa actagtcaag acacatatat aaataagttg tttgagcaat atgatatgac
     9241 cttagtacaa gcattggaaa ttaaagatga agatttggat atagatagaa aattcttaga
     9301 aagtctaaag agagaaataa gaagtcttgg aaatataaat atagactcta taaaggaata
     9361 tgaggaaata aaagaaagat acgattttta tagcgaacaa aagcaagatt tagaagagtc
     9421 tatggaagag atagaaaaac ttatacatac tttagaagaa aatatgaaat ctgaatttga
     9481 aattaagttt gaagaaataa gtaaaaattt taaatatgta tataagagat tatttggtgg
     9541 aggttgtgga gaattaacta ttttagataa agaaaattta ttagaaagtg atatactaat
     9601 aacagctcaa cccccaggta aaaaaatgaa aaatttaaac ctattatctg gtggagagaa
     9661 agctttaacg gcaataagta tattatttgc tatattgatt acaaagccaa caccattttg
     9721 tatattagat gagattgaag cgccacttga tgacgcaaat atatttagat ttggagaatt
     9781 tttgaaggat ttatctaaag agactcaatt tatatcagta actcatagga gaggaacaat
     9841 ggaagctgct gattatatct acggagttac aatgcaagaa aaagccattt caaaagttat
     9901 tagtttaaaa ttaaaagaag ctcaggaaat aacagatatt atataactta gattgacata
     9961 ggaggaataa cagtgttaaa aaaattattt ggttttggaa aagacaagga aaaggaaata
    10021 gaaaaaaaag atgctgaaga agaaatagaa gttgaagaca gtgttgataa tttagaaaac
    10081 ttggaagaaa ctattttttc aggacttgaa gaagaagtga tagataaagt ggaagatatt
    10141 gaagaaaaaa atgaggaaag tgtcaataat gaaggcactg aagaaatcga aaactttgaa
    10201 aaaatagaag ttgagatgga tagtaatgga aaagaagtag aaaatatttc aaataatgat
    10261 tctcaagaag agataatcga agaattagaa aattatgatg aaattgagtc taaagagata
    10321 gaagataaag aagataagaa agttaacttg tttgaaagac taaaacaagg attgacaaaa
    10381 gcaaagcaag gaataactga tagaatagat gaagttttaa aatcatatac aaaaatagat
    10441 gaagaattac ttgaagattt agaggaaatt ttaataacag cagatgttgg tgtaaataca
    10501 actatggata taattgaaag actaagagat aaaataaagc aaaaaggtat tactgaacca
    10561 ataaaggtaa gagaagaact taaatctata gttgaagata ttcttacaaa tgaaaactct
    10621 acactagata tagaaccagc accatgtata atattaatgg tgggtgtaaa tggggtcggt
    10681 aagactacta caataggtaa actagcaaat agatacaaaa aagatggtaa aaaagtttta
    10741 ttagcagcag cagatacatt tagagcagca gcgacagagc aattagaaat ttgggcaaat
    10801 agaaccaatg tagacataat aaaacatcaa gagggagcag accctggagc tgtagtattt
    10861 gatgctataa aagctgcaaa agctagaaaa acagatgtat taatatgtga tacagcagga
    10921 agattacaca ataaggctaa tcttatgaat gaacttggaa aagtatttaa aatagtagat
    10981 agagaatttc cagaagcaaa acgtgaagta cttcttgtgg tggatgctac aacaggtcaa
    11041 aatgcagttg ttcaagcaaa gacatttaaa gaagttgcag atataacagg tatagtactt
    11101 acaaaacttg atggaactgc aaaaggtgga gtagtccttg cagtaaaatc agaagtagat
    11161 gtacctgtaa aacttattgg agttggagaa agtgtagaag acctacaaga tttcaatgca
    11221 aaatcatttt cagatgcatt atttggtaac taatgttctt ttaaaaataa tggttgacaa
    11281 gagtgttgtt atgatataaa atacaatagt gtaaagtaaa taacctttac agtatactgc
    11341 taggaagtgt aagatatgaa tatagaaaaa atggtcgaaa ttggattgtt atttgaacag
    11401 tataaagaat tattaactga caagcagaaa gaaatagttg ctctatacta tgaagaagat
    11461 tattctttag gtgaaattag tgaaaatctg aatgtatcaa gacaaggtgt ttatgatacc
    11521 ttaaagcgtt cagaaaaaat actaagagat tatgaagaaa agttacactt agtatcaaag
    11581 attcaagagc aggaaaaaaa tattaagatt ataaaagata aaattattga cattaaagaa
    11641 gatttattgc acaatagaga ttgtgctaat ttaatcccta agctagaaaa tatagaagat
    11701 gtatgtaggg agatgataaa atgatatttg aaggattgtc tgataaacta caaggggcat
    11761 ttagtaagtt aaaatctaaa ggaaaactta cagaagtaga tgtaaaaaat gctatgagag
    11821 aagttaaact tgctcttctt gaggcagatg ttaactttaa agtagttaaa gatttcataa
    11881 aaaaagttca agagagatgt gttgggcaag aagttatgca aagcttaact ccagctcagc
    11941 atgtaatcaa aattgtaaat gaagagctta caagtttaat gggagatgta caaagtaaag
    12001 taatgatttc ctcaaagcct cctacaattt taatgatggt tggattgcaa ggtgcaggta
    12061 agactactac agcaggaaaa cttggaggat attttaaaaa gcaaggtaaa aaacctctat
    12121 tagtagcttg tgatatttat agaccagcag ctataaagca acttcaagtt gttggagaaa
    12181 aattggatat acctgtattc aatatgggag ataaagaaaa tccaataaat attgcgaaag
    12241 caggtttaag ccatgcgatt aaaaatgcta atgatttagt tattatagat acagctggta
    12301 gacttcatat agatgaagta cttatggatg agttaaagtc tattaaatca gaagttaagc
    12361 cacatgagat acttttggtt gtagattcta tgacaggtca agatgctgta aatgttgcag
    12421 aaagcttcaa tgaggcactt ggtgttgatg gagtagtatt aactaaattg gatggagata
    12481 ctagaggtgg tgctgcgctt tcaataagag cagttacaca aaagcctata aaatttatgg
    12541 ggatgggaga aaagctagat gatatagaac ctttccaccc agatagaatg gcatcaagaa
    12601 tattaggtat gggagatgtc ttaagtttaa tagaaaaagc acaagaaaat atagacttag
    12661 agaaagctaa agaattagag caaaaaatta aaaaacaaga gcttgatttt gaagatttct
    12721 tacaacaaat ggaacaaata caaaatatgg gacctcttga caagatactt ggtatgatac
    12781 ctggtatggg aaatgtaaaa gaccaacttg gagatgtaga tttgaatggc aaagaaatga
    12841 agagaacaaa agccataatt cagtcaatga ctacatatga gagaagaaac ccaggagtat
    12901 taaatgcttc aagaaagaaa agaatagcta aaggcagtgg aactagtgta caagatgtaa
    12961 atagacttat aaaacaactt aatgaaatga aaaaaatgat gaagatgttt gctggttcac
    13021 aaaagagtat gaagaaaagg ggcggcttag gcggtttacc tttttttaaa taaaatataa
    13081 aaaagtaaaa attattcgga ggtgactgta atggcagtta aaataagatt aaaaagaatg
    13141 ggagctaaca aaaaaccttt ctacagaata gtagtagctg attcaagagc tccaagagat
    13201 ggaaaattta tagaagaaat aggatactat aatccaattt ctgaacctaa acaagtaaga
    13261 ataaatgatg aaaaagctat aaaatggtta gctactggtg ctcagccaac agaagtagtt
    13321 aaaaaattac ttgtaaaaaa cggagtaata gaaaaattcg aagcttctaa acaagcaaaa
    13381 taattttttc ttggcaatta gtttaagtaa gggctcattg ctaaggttac tttaggagat
    13441 gaaatatatg aaagagctag ttgtagatat agctaaggct ctagttgata atcctgactc
    13501 agttgttgtt gaggaatttg aagataatga tggtatcgtc ttaaagctta cagttgctca
    13561 agaggatatg ggtaaggtta ttgggaagca gggaagaata gctaaagcta taagaactgt
    13621 cgtaagatct gttgcaaata gagaaaatat aaaagtttct cttgagatag tataaaatag
    13681 gaggattagg tataatgcct agtccttttt atataaatgc gtagtttaag attttatgat
    13741 gatatgacca cataagatta aattttttaa gaaggtgata acatgaaaga aaaattaact
    13801 cattttaagg taggtcaaat agtaaataca caaggtttaa aaggtgaagt aagagtttat
    13861 ccgcttactg atgatataga gaggtttgat gaattaaaag atttctatct gggaaaagat
    13921 ttaaataata aattggctgt tgaaaaagta agatacaaag gttctatggt tataatgaag
    13981 ataaaaggca tagacagcat agagaaagca gaaaaactaa aaaataaatt tatgtatgta
    14041 gctagagaag atagcagaga gcttgaagaa ggcgaatttt ttatagcaga tatgattgga
    14101 atggaagttt taactgtaga tggaaaatat gtagggatac tagaagatgt tcttcaatac
    14161 tcagcaaatg atgtctatgt agtaaaaggt gaggaagata aagaatttat gataccagct
    14221 ataaaaaaat ttgtacctac aatagatatt gatgaaagaa aaatgattat agaccctatt
    14281 aagggaatga tagattaggt ggtaatatga gatttcatat aatgacactt tttccagaaa
    14341 tatttaattc gtacatggat gaaagtataa tgaagagagc agttgaaaaa ggtataattg
    14401 aggttcatat atataatata agggattttt ctaataataa acataaaaaa gtagatgatt
    14461 atccatttgg aggaggagcg gggatggtaa tgactcctca acctatatat gacacatata
    14521 agcatataat aacaactcat aatataaata acccaagtgt aatttatctt actccaaaag
    14581 gtaaggtata caatcaatct atggcaaagc agatgtcttt aaaagaagat ataatactac
    14641 tatgtggtca ttatgaagga atagatgaga gaatcattga tttaatagtt actgatgaaa
    14701 tttcaattgg agattatgta ttaactgggg gagagctccc agcacttata atgatagatt
    14761 ctatatcgag attaatacca ggtgtattaa accaagaaga atcatttgaa gaagaatcat
    14821 ttaaagacaa cttattggaa tatcctcatt acactagacc tagagatttt gaaggattaa
    14881 aagtaccaga agtgctttta tcagggaatc ataagaagat agatgagtgg agaagagaag
    14941 aatctataag gataaccaaa gaaagacgat ttgatttata taaaaaatct aatgaaaaat
    15001 aaaaatataa tgaaaaatta atatatatgt atacaataaa gtacctataa atggaattaa
    15061 aaacataaaa gtgcttgtaa taactattga agtatagtat aatataaaat gttgaaaata
    15121 cgggcgttcc tctgtttaca ctggtcgggt agttaagaac gtctatgata tctaggagga
    15181 agaaagatga atgaaatgct aagagcaatt gagcaagaac aattaaaaaa tgaagttcct
    15241 aattttggac ctggggacac agttaaagta cacgttagaa ttatagaagg aaaaagagaa
    15301 agaatacaag tattcgaagg tgtagtatta aagagacaag gtggaggagc aagagaaact
    15361 ttcacagtta gaaaaatgtc tttcaatgtt ggtgtagaaa gaactttccc agttcactct
    15421 ccaaaaatag aaaaaataga agttactaga aaaggtaaag taagaagagc taaactaaac
    15481 tacttaagag gtagagtagg taaagctgct aagataaaag aagctagaaa taaataatta
    15541 aaaactttac ttataaatgg ggggctgatt tatcagtcct ctttctattt taatgaaaat
    15601 tacagcaaat aattatacta gaaataaata gaaatatttg ttgaaaatat acattattta
    15661 tctatgaatt tatagctttt aaaatgaaaa ataaaattgg taaatattgc attatatttt
    15721 gtaacaatat taaaaataag agaacagtat tatgtaaagt atgacgttaa aaagatgaca
    15781 atattatagt ttgttaagat agatttttta agtggaatta ggatataata atataatatt
    15841 aataaggagt gatacatgtg tcaaatgaat acgaagagta tcttatgaat gacaatctac
    15901 atataaactg gtatccaggt catatgaaaa aaactaagga gttagtaaaa aataacttaa
    15961 aattgattga tgtagtaatt gaacttttgg atgctagaat cccgtttagt agtaaaaatc
    16021 cagacataga caggcttgta ggagataagc caagagttgt cgtacttaat aagagtgata
    16081 tggcagatag ggataaattg aatcaatgga ttgaatacta taagaaaatt aatatcaaag
    16141 ctataccagt agatacaata aaaggcgttg gtataaataa gataatcgaa gaatgtaaaa
    16201 atgtaactag agaaaaaatg agttctttaa aagacaaagg gagaaaagaa agagctatac
    16261 gaataatgat agttggagtt ccaaacgttg gtaaatcgtc tcttataaac aaattaacag
    16321 gtagaaaaag cactcaaact ggagataagc caggagtaac gaaagggaaa caatgggtta
    16381 gattaaaggg aaatcttgaa cttttagata cacctggaat actttggcct aagtttgaag
    16441 accaagaagt tgcattaaat ttagcattta gtagagctat aaaggatgag attttagata
    16501 ctgaaacttt ggctttaaga cttatagaga aacttatgaa aatagaacct gagaagttga
    16561 aagccagata taagttagat tgtctaggag aaactccaat tgaaactatg gatatgattg
    16621 gccataaaag agggtttata acaggtaaaa aagaactaga ttatacacgc atagccacaa
    16681 ctgtattaaa tgaatttaga gatggaaaaa taggaaatat aacattggaa gttccagaaa
    16741 acgttaaaag atagatttta aaacaaaatc aaaaatttaa tgttaaaaat ttaaaaaagc
    16801 cttttagcaa aggctttttt atttttttga aatatagaca aatatctcca tataaaagta
    16861 taaattaggt tagaaaacct ttaaaaatat taatttaaga caaatatacc taatattttg
    16921 aaaattaaaa tgagtgtgat tatttaaaat tttagcatat tgacttgctt gtttcgtata
    16981 atatataata agcacaaata aatacaatat attaaaaatt taatttattt aattttatta
    17041 atttaataat aagttcttat tgtttaattc aacttactta tgcaagcgta aacaatcgag
    17101 acattttatt aatttaataa ggttaaaaag ggggatttta taatgggtaa gaccaaggat
    17161 gttatagtat ttggatttgc gttatttgca atgttctttg gagcaggtaa cttaatattt
    17221 ccaccttact tgggaataat tacaggtcca gaatggttaa ttgccttctt aggatttacg
    17281 tttgctgatg ctggattggc acttctagca gtaatggcta cagctaaatt cgatggaaac
    17341 gtagttgaaa tgtttaaaag atgtggtatg aagttaggaa ttcttatagg ttgtgcagat
    17401 attttatgta taggaccatt cttagctata ccaagaacag gtgcaacaac ttatgaaatg
    17461 ggtataatgc ctttgtttgg aacttcaatt ccagtgttat tattctgtat actttttttt
    17521 gcgatttcat atgtactaac aataagacct tctaaggtcg tagatatagt tggacagttt
    17581 ttaactccag ctcttctaat agctttagca tttataatta taaaaggtat tatatcacct
    17641 cttggagata tagtagataa acctatgata cctaatgtat ttgcagaagg tataggtcaa
    17701 ggatatcaga caatggatgc ttttgcagca atagcacttg catcagtgct aatagtatct
    17761 ttaaatgata aaggatattc tactattagt gacaagttga aaatgattgg taaggcgggt
    17821 gttcttgctt gtggaggatt agctctagtt tatggaggat tatgtttcct tggggcaact
    17881 gtatcaacaa tgtatggaac agatgcagta caatcacaag taatagtaaa tataactgaa
    17941 ggattgcttg gaaatgttgg gaaagcgatt ttagcagtag tagtatcatt agcatgttta
    18001 actacatcta taggtcttac atctgccaca ggtcaatatt tctctagact tacaaaaggt
    18061 aaattgagtt atgaaaaaat agtattagct gtatctgtat tcagtgcagt agtagcatct
    18121 tttggtgttg gaactataat aaagatagca tctcctatac taagtatagt ttatccacca
    18181 tcaattgttt taataatttt agctttcttc aatgaaaaaa ttaagaatga taatgtatac
    18241 aaaggagcag tatatatgtc attgtttgta agtatactaa cagttatatc tagttatgga
    18301 gtagctgttc cagtagtaaa ttctttacct cttaattcat taggatttaa ctgggtggtg
    18361 ccagttataa tagctggaat aattggtaat tttataccat caaagagtca atctaataca
    18421 cttggaacaa actaagtttt gtttaattaa aatataatca at
//
LOCUS       DAYAOX010000062        18450 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.062, whole genome shotgun sequence.
ACCESSION   DAYAOX010000062 DAYAOX010000000
VERSION     DAYAOX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.062"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            494..1867
                     /locus_tag="ACGI57_001690"
     CDS             494..1867
                     /locus_tag="ACGI57_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9716770.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGREITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            2123..3190
                     /gene="ord"
                     /locus_tag="ACGI57_001691"
     CDS             2123..3190
                     /gene="ord"
                     /locus_tag="ACGI57_001691"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HFG9716771.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            3334..3642
                     /gene="ortA"
                     /locus_tag="ACGI57_001692"
     CDS             3334..3642
                     /gene="ortA"
                     /locus_tag="ACGI57_001692"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HFG9716772.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3635..5065
                     /gene="ortB"
                     /locus_tag="ACGI57_001693"
     CDS             3635..5065
                     /gene="ortB"
                     /locus_tag="ACGI57_001693"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HFG9716773.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSYFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            5143..5517
                     /locus_tag="ACGI57_001694"
     CDS             5143..5517
                     /locus_tag="ACGI57_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HFG9716774.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5511..7715
                     /gene="oraE"
                     /locus_tag="ACGI57_001695"
     CDS             5511..7715
                     /gene="oraE"
                     /locus_tag="ACGI57_001695"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HFG9716775.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7715..9124
                     /locus_tag="ACGI57_001696"
     CDS             7715..9124
                     /locus_tag="ACGI57_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HFG9716776.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            9109..10173
                     /gene="orr"
                     /locus_tag="ACGI57_001697"
     CDS             9109..10173
                     /gene="orr"
                     /locus_tag="ACGI57_001697"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HFG9716777.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            10203..11624
                     /gene="nhaC"
                     /locus_tag="ACGI57_001698"
     CDS             10203..11624
                     /gene="nhaC"
                     /locus_tag="ACGI57_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFG9716778.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            12053..12517
                     /locus_tag="ACGI57_001699"
     CDS             12053..12517
                     /locus_tag="ACGI57_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9716779.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12751..13113
                     /locus_tag="ACGI57_001700"
     CDS             12751..13113
                     /locus_tag="ACGI57_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9716780.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13708..14568
                     /locus_tag="ACGI57_001701"
     CDS             13708..14568
                     /locus_tag="ACGI57_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9716781.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            14826..16940
                     /locus_tag="ACGI57_001702"
     CDS             14826..16940
                     /locus_tag="ACGI57_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFG9716782.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            17481..18434
                     /gene="blaCDD"
                     /locus_tag="ACGI57_001703"
     CDS             17481..18434
                     /gene="blaCDD"
                     /locus_tag="ACGI57_001703"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HFG9716783.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT         7352 a         1991 c         3390 g         5717 t
ORIGIN      
        1 aaaaatctag attttacata ccaaactggt ttcttatttc gctatcttta cttatgaact
       61 catatattcc tataaaggtt attttagaaa aagttggtta tttaaacatt aataccacaa
      121 tttctataga ttgtttggtg aataatctat aattatatta tggactaaaa aatccaatga
      181 gctaatttat tgttttttta tatttgagag attacaactt attagtttgt atatctcttt
      241 ttttaataaa atcaataata tataaaacta ttggcatatt taatgacaat tcaaatattg
      301 tatataacca taatgaaaga tatataaata aaattttaaa tatacttaaa aatatattaa
      361 tataatgtac ataaaaaata tcataaatat atgataagat aaatacaata gcaacatttt
      421 attaattttt tgggttttta tatatttagg tactggggtg atatagttga accaagagat
      481 attttttaaa ataatgaatg aaatattgga aaatattgat gaaggggtac attttgttaa
      541 taaagacggg aaaactataa tctacaataa ttctgtacaa aaaatggaaa aaatgaacag
      601 acacgatatt ttaaccaatt cattttttga tatagtcaac aaaatgaaaa taaacaggag
      661 tacactatta gaagttttgg aaaaaaggga agttatcaaa gacaatattc aaaaatatct
      721 ggataaaaat ggaagagaga taacagcaat taatactaca ataccaattg atattaaagg
      781 cgagtttgta ggggcacttg agatatcaaa gaatatgaca acgatagaaa atttactaaa
      841 cagaaattat aaaaaaaatt tagtcaataa agttagtatt agacaaaaaa agggttatga
      901 atttgatgat attttagggg aaagtaattt gattaaagaa gcaatagcta aatctaagaa
      961 agcttgcaag tctgatgctt cagtatttat atacggtgaa actggaacgg gtaaagagtt
     1021 atttagccag tccattcact atggaagtaa taggaaaaac tatccattta tagcacaaaa
     1081 ttgtgcagct ttgccggaat cactattcga aggtattctt tttggaacta gcaagggagg
     1141 atttacagga gcaatagata ggccaggctt atttgaacaa gctaacaaag ggacattgtt
     1201 attggatgag ataaattcca tgcctattat gttacaggct aaactattaa gggttttaca
     1261 agaaggttat gtaagaaggg ttggaggaac taaggatata cctatagatg taagaattat
     1321 tgcaacgaca aatgaaagtc caaaggtaat tttaaacgag cataaaatgc gaaaagattt
     1381 atattatagg cttaacataa ttcatataaa aattcctcct cttagagata gaaaagagga
     1441 catacctcta ttgtgcaaaa aatttatttc aaagtataat aaaaagttaa ataaaaaagt
     1501 agtaggaatt tcagatgaag ctattaaaat tttaaaaaat tacgattggc aaggtaacat
     1561 aagagagcta gaaaacataa tttattgtac tatgagtatg atggaagatg aggtacaaat
     1621 agaaacaagt atgattaatc taaacgacta ttacaatctt aaagagaata aagagagtaa
     1681 caattattct ttaaacgatt tggaggacaa aacacttagt aagattatca gtgagataga
     1741 agaaaagtat atatatgaat cattagaaaa ggcatcttac aatataacta aagctgcatc
     1801 aattttaggt atgaataggc aaaatttaca gtataaaata aaaaaatata atatcaaaat
     1861 attataaata agcaagataa aaaaacaaaa tcaaagtaaa ataaaaaagc aaaatagagg
     1921 agcaaaatta ttataaaaga gcaaaatatt ttgctctttt ttttatttct cataagaaat
     1981 attatttttt atttatgaaa atacataata aaataattat agtttcaacg tttcaataaa
     2041 tatttacttt aattttaaag ttggcacgat ttatgcttta actaattatt gagaatatat
     2101 aaatttttag gagggtgtat atatgagaaa agtaagagta ggaatatggg gatttggagc
     2161 tatgggaata ggtatggcta atatgattct aaagaaagaa ggtatagaaa tagtatctgt
     2221 ttgtagtaga agtacttcag gaaaaagtat gtataatgtt ttaggaattg aaagaggaga
     2281 aagaccagaa gttattatca ataaaaatta tgaagaagtt tttagagaaa aaagtgtaga
     2341 tgttgttttg ttagcaactg attcatttac taaaaaggct tttgataaaa taattttctt
     2401 attaaataga aaaataaatg ttatatcaac agcagaacaa atggcatatc cacaagctga
     2461 tgatgcagat ttagctaaaa aaatggatga agttgcaaag gaaaatggag taagtatact
     2521 tggaacagga ataaatccag gatttgtatt agatttacta gtattggcgc tttcaggcac
     2581 ttgtgaagaa gttactagta taaaagctaa gagagtaaat gatttatctc catttggaaa
     2641 gtctgttatg gaggaacaag gtgttggagt aacaagggaa gaatttataa aaggtgtaga
     2701 agataagact atagcaggtc atgtaggttt tgtagaatca ataaatatga tagctgatgg
     2761 attaggttgg aaattagata agatagaaca aacaaaagag ccaataatga ctacagtaga
     2821 tagaaaatct aagtacggag aggcattggc tggaaatgtt gctggttgta gacaatgtgg
     2881 atatggatat gtaaatggag aagttttaat tgaaatggag catccacaac agataatacc
     2941 agaagcagaa ggtattaaaa caggagacta tgtatcaata aaaggtatac caaacattga
     3001 tttacaaata aatcctgaaa tacctggagg agtaggtact tatgcaatga tagtaaactc
     3061 tattccatta ataataaatg ctagaccagg tcttaaaact atgttggata taccagtacc
     3121 tagagcaata atgggagata taagaaatca gatagaagtt gaattggaag aagaatcaaa
     3181 ggctaattag taagataaca gctaactaat tagtaagata gcaactagct aattagtaag
     3241 ataacagtta gctaattggt aaaataatgt ttaatttaaa ataacagtaa attaattagt
     3301 aaggcaatag tcaattaata aggggatgaa aaaatggatg ctaaaattaa tgattgggtt
     3361 attatacaca atatagtatt gactccagaa gaaagagctc cacaagttcc agaggatact
     3421 aagaaagtat ccttagaaat gtgggttaaa ggttttatac aaagtgatgc ttctataggc
     3481 gacttagtag aagttaaaac aattacaggc agacttgtaa aaggtaattt acttaaaata
     3541 aatccatatt atactcatga ttatggtaaa tgtataccag agttactcca aataggaatc
     3601 caagctaaag aaatattatt tggaggtgta tataatgaat aattcaacat caatgaaaga
     3661 tatgagttat caagctgtta tgggaagaaa taacgaaata atgaaaaatg caatcggatt
     3721 ggattattct tattttgaac aagagggaat agggtttgat tatgagaaaa tgatgagtga
     3781 aacaggatat acattacaag atattgaagc aattcaatca cagtatgctg ttggaaatac
     3841 tcctttaata gagcttaaaa atttgactaa attagctaga aaatgtgcta aagaaggtaa
     3901 aggagctaga atatttgtaa aagatgaagc aatgaatgca tctggtagtt ttaaagcaag
     3961 aagagcagct actgcagtat atcatgccaa acaaatggga tataaaggtg taatagcagc
     4021 tactagtggt aactatggtg cagctgtagc atcacaagcg gcaatgcagg gattaaaatg
     4081 tataatagtt caagaatgtt atgattctaa tggtgtaggt caacctgaaa ttatagaaaa
     4141 agcacgaaaa tgtgaggcat atggagcaga agttgttcag ttaactgttg gaccagagtt
     4201 attttatacg ttcttagttt tattagaaga aacaggctat tttaacgcat cattatattc
     4261 accatttgga attgcaggtg tagaaacttt aggttatgag ttagctatcc aatttagaga
     4321 aaagtataaa aaagaccctg atatagttgt ttgtacaaat gcaggtggtg gtaatcttac
     4381 tggaactgca agaggattga taaaagctgg ttctattaat actaaagtag taggagcaag
     4441 tgtagattta aaaggattac acatggctag tgataatcaa tttaataaaa aatcatttac
     4501 aacaggacat acaggttttg ggataccata ctgtacatgg cctgataggt cagatgtacc
     4561 taggtctgca gctagaccac ttagatatat ggatagatat gtattagtaa aacaaggaga
     4621 agttttttat acaactgaat tattggcaca gctagaaggt atagaaagag gtccagcagg
     4681 aaatacatct ttagcagcag cattctcttt agctcaagag ttagatgagg ataaagttat
     4741 agtggtacaa gaaacagaat atacaggcgc aggtaaacat atatgtccac agcttacttt
     4801 tgcaagagaa aatggaatag atataaaatt tggaaatcca agagaagaaa ttgcaggagt
     4861 aaacttaata ttaccagaaa gaccagaatt attgaaatgt gttgatattg atatgaataa
     4921 aataagaaag tcatttataa aaaattgtat atctaataat cacattgata atattgataa
     4981 gctaagtaat cgagatatag aatttttaat ggaagaagta aaaagttcaa gagattttgt
     5041 tgttgatgta ttaaataatt tatagtaatt attgtttttt aatttaatat aaaataaaaa
     5101 ttcagaaaat tcgattaaat caatacgaat ggaggaatgt tcatgaaaaa aagagaggat
     5161 gattttgaag ttagaagaaa acatcttcaa gaactgagtg atgatgagtt aaaagaaagg
     5221 ttttggagtt tagctaccca gatagtagaa ccaatgatag aacttggtaa aaagaataca
     5281 acaccatcaa tagaaaggtc tgtcttgttg agaatgggat tttcttctct tgaagtaaaa
     5341 cctattttag aaggggttat ggaaagaggt cttataggta aaggagcagg gcatgtagtt
     5401 tataaattgg ctaaatctaa aaatataacg gttagagaag caggtttgtt attagttaag
     5461 ggtgaatatt gggatgaagt tacatgttta tttagagaag gggtagaatc atgttaaagg
     5521 aaaataaaaa attggatata gactatatat taaaagattt ggataaatat aaaccaaaac
     5581 gaagaggttg ggtttggaga gaacacttgg aaaatcttga aatgggacct tttaaatata
     5641 aagactgtac aaaaccttta aagaagagtg tagggctacc atcatctaag tatttcaata
     5701 atatagaccc tcaaccaagt ccagttatta ctactgaaat agcttctggt agatttgagg
     5761 atgatataag aagaatgaga atggcagctc atcatggagc tgaccatcta atggttatca
     5821 gaactgctgg acaatcacat tttgatgggc ttatagaagg aactccacaa ggtataggtg
     5881 gagttccaat aacaagaaaa caagtaagag ctcaaaggaa ggctcttgat tttatagaag
     5941 aagaagttgg tagacctatt aattaccatt catatgtaag tggtgttgct ggtcctgaag
     6001 tagcagtaat gtttgctgag gaaggagtca atggagcaca tcaagaccca caatataatg
     6061 ttctgtatag gaatataaac atggtaaggt cttttgtaga tgcttgtgag gcaaagaaaa
     6121 taatggcttt tgccaatata gctcaaattg atggagcaca taatgctaat gctactgcta
     6181 gagaagcatg gaaggtaatg cctgaactta tggttcaaca cgctttaaac tctatatttt
     6241 cagaaaaaat aggtatagat aaaagtaata tatgcttatc aacagttcca ccaacagcac
     6301 ctccagcacc atgtttaaaa atagacttac cttacgctgt tgcacttaga gagttattta
     6361 gtgattataa gatgagagct caaatgaata ctaaatatat ggaatcatca actagagaag
     6421 caacagttac tcatgtatta aacttattaa catcagtatt aactagagct gacatacaat
     6481 caactataac acctgatgaa ggtagaaatg taccttggca tatatacaat atagaagcat
     6541 gtgatacagc aaagcaagct ttagttggta tggatgggct tatggatatg atagaactta
     6601 aagatgtggg tgaattaaga gacaaagcaa gagaacttaa agaaagagct gtattatata
     6661 tggaagaaat atctgaagtt ggtggatatt ttgaatctgt ggaacaagga ttttttgttg
     6721 attctggaaa ttatccagaa agaaatggtg atggaatatc tagaaaaata aaaggtggag
     6781 ttggtgaagg tactgtttat gaaagagaag aagattattt agcacctgta actgctcact
     6841 ttggatacaa taatgtagct caatatgatg aaaatgctat agataatcca tcaatgttaa
     6901 tagatggatg tacatttgaa aatccagaca aaataatata tattgatgag cttgacgatt
     6961 ttgataatgt agaaaataga ctaaaagaat catatgagta cagaaatgga actaaaatta
     7021 agcctgaaat ggaatggtgt gcagatggaa ttgtaatgat aacaatgatg cttcctactg
     7081 ataaaagaac agcagagttt gcagctttgg aatttgtcaa aaaaatgaac ttacaagaaa
     7141 ttgaggtcat aagtagagaa gttatgcatg aatctgaagg gactagaata gaagtgaaag
     7201 gaagagttcc gtttgatata gatttaaaca atttagtaat accagaagaa cctaaggtac
     7261 taactgatga agaaataaga gcagatatag aagaaaaacc tatgaaaata gtttcagcta
     7321 cggttggaga agatgaacat tcagttggtt taagagaaat aatagatata aaacatggag
     7381 gtattgaaaa atatggcata gaatgccact atctagggac ttctgtacct gtagaaaaat
     7441 tggtagatgc agcgattgaa ctgaatgcag atgcaatact tgcatctaca ataataagtc
     7501 atgatgatat tcactataaa aacatgaaaa aattacacga ttattgtgta gaaaaaggta
     7561 taagagataa agtaatgatt gcgtgtggag gaacacaagt aacacctgaa atagctgttg
     7621 aacaaggcat agatgctgga tttggaagaa acagtaaagg catacatgta gccactttct
     7681 tagttgaaaa aagaagagaa atgagtaata aataatggaa aatagcaagg tttctttaaa
     7741 aatagatgtg ttggtagctg aaattggaag tactactaca gtggtaaatg ctttccacga
     7801 tattaatact aacaatccta tatttttggg tcaaggtcaa gctccaacaa cagtgtttga
     7861 aggtggcgat gtaagaaatg gcttatcagg tgctattaaa gatttagcta ataaattatc
     7921 tgtagatgat atagagtata atgatatgtt tgcaacttct agtgcagctg gtggattaaa
     7981 aatgactgtt catggattag tctatgatat gacagtaaaa gcagctaaag aagcagcttt
     8041 aggggctgga gctgttattc gtcaaataac atcaggtaga attaaaagaa ctgatttaaa
     8101 taaaataaaa gaaataaacc caaacataat tttaatagct ggtggagtcg attatggaga
     8161 aagagataca gctatttata atgcagaaat gatagcttct atgaatctag gcataccagt
     8221 tatatatgct ggtaatgttg aaaatcaaga ggaaataagg ttaatatttg aagatactaa
     8281 ttataagctt tatataactg aaaatgtata ccctaaaata gatttattga atatagaacc
     8341 tacaagaaga atcattcaaa gtgtatttga agaacacata actacagcac caggaatgaa
     8401 atacattaaa gaaatggtta atgaaaatat tactccaacc ccaggagctg taatggaagc
     8461 ttctaaactt ttatataaaa atattggaga tttattaact ctggatgtag gtggagcaac
     8521 tactgatgta cattctgtta cagatggaag tgactatatt aataaaattt tggttaatcc
     8581 tgaacctact gcaaagagaa gtgttgaggg ggatttaggt gtatatgtaa atatgaagaa
     8641 tatagtagaa gttataggta aagaaaactt acaaagtgaa ctatctatag atattgatgc
     8701 agtaatagag aattatcctc caataccaaa atcaaaagaa gaaatattat ttgttgagag
     8761 gttgacaaaa gaagctgttg ttaaagctat gctaagacat tcaggaaaaa taagaaatat
     8821 ctatggaaca actggaaaag tcaaaattgc agaagggaaa gatttaacag aagtaagata
     8881 tatagttggt acagggggag cacttactag gctccctagt agaattgaaa tattagataa
     8941 tatgctcaaa tacaataaaa ataatgaatt actatttcca aaggaaaaaa caaagatatt
     9001 aatagataat gactatatta tggcatctat gggtgtttta tctaaaaaat atgaagaagc
     9061 atctcttaag ctcctgttaa aaagtttgaa ttttgaggag gaaagattat gtatcctagg
     9121 ttagaaatag atatagaaaa gttaaagcat aatgcaaaat taatttcgca gatgtgtcat
     9181 gaaagaaata ttaagatatc ttttgttact aaatcatttt gtgcacaaaa agaaatagta
     9241 gaagaaatat gtagtgaagg aatagaccat atagcagatt caagaataca aaacttaaaa
     9301 aatttacaag atataaatct acctaagata ttgattagaa tcccaatgtt aagtgaatta
     9361 gaagaagtta tcaattattg cgatattagt tttaactcag aattaggaac aataaagaaa
     9421 ttaaatgaat tatgtgaaag taaaaatatt attcataaga tagttcttat gtttgactta
     9481 ggcgatttac gagaaggata cttttatgaa gaagactttt ttagtaatgt aagagagata
     9541 gtaagattaa aaaatattga aataattggt atagctacaa acttaacctg ttatggagct
     9601 attataccat ctagggaaaa tataggcaga ctggtaagta tagcaaaaag aatggaagaa
     9661 aaatttggaa taaatctaga aatagtatct ggaggtaatt caagttctat tcatctgttg
     9721 ataaataata atatgccaga aggtattact aatttgagaa taggcgagtc cattctactt
     9781 ggtagagaaa cggcatacgg tgaaaatata aatggaacat atcaagatgc atttaaatta
     9841 atatgccaag tagttgaatg taaagaaaaa ccatcagttc ctattggaga aataggagtt
     9901 gatgccttta gaaataaacc tgtttatgaa gataagggaa tcttaaaaag ggcaatcata
     9961 gctataggta aacaagatat aaatattgac tctttaattc caatagacac tgatataaaa
    10021 atattagggg caagttcaga ccacatgata ctagatgtaa gtaacacaaa atatgattat
    10081 aaattaggtg acaatttaga atttttatta acttatgggg gtatcatgtc atctagtacg
    10141 agcaaatatg tttcaaaaaa aattatagtg tagtgtttaa ataattagga ggaaactatt
    10201 ttatgaaaga gcgagaatgt agaaaagcaa gtctgtttga tgcaatgata ccaattactt
    10261 gtttgatatt atttttaagt ctaggagtat tagtgtatgg ttcatcacct catgtaccac
    10321 taataggagc agcagcagta gcaggacttg tggctgtata tagacttgga tttaaatgga
    10381 aagagttaga attaagtatg tttaatagta taaagatggc aatgcaagca atcttaatca
    10441 taattataat aggcgtactt ataggaactt ggatagtaag tggagtagtt ccatgtatga
    10501 tttactgggg actaaaaatt ttatcaccaa atattttttt agtagcatca acattagtct
    10561 gtgcaatagt atctcttgcg acaggttcat cttggagcac aatgggtaca gtaggagtag
    10621 ctcttttagg cattggtcaa agtttaggta tgccaattgg attaatcgta ggttctataa
    10681 tatctggagc ttactttgga gataaactat cacctttatc tgaaacgact aacttagctc
    10741 cagcaatggc aggaacagat ttgtttacac atataaaata tatgctatat tcgactatac
    10801 catcattatt aatttgttta gtaatttatg gtgtaattgg aatgaagtat tcaggtcaag
    10861 cactagatat aaaacaaata gaattaatta ggtctacatt agattctaca tttaatactt
    10921 tatcaccagt attattatta gctccagcaa tagtaattgg attggttgta tttaaagttc
    10981 ctgctatacc agggcttata gtaggtgctg tattgggagt attgtttgca gtagtttttc
    11041 aaggagaatc tatgaatacg atattagatg cagcacaaaa tggatatgtt tcgtcaacac
    11101 aaatacagga ggtagatgct ttattatcta aaggtgggat tatgaatatg atgtccactg
    11161 tatccttaac aatatgcgct ttatctcttg gaggtatttt agaaaaaaca ggtatgcttg
    11221 aagtagtagc atcttcttta ctaagattag caaagggtgt atttggaaca gtattatgta
    11281 ctatggtaac ttgtacaatt accaatataa tagcaggtga acaatatcta tctatagtta
    11341 taccaggaag aatgtataat aaggaatata aaaagagagg tatacatcct aagatgttgt
    11401 cgagagcttt agaagatagt ggaactttaa cctctccatt agtaccatgg aatacatgcg
    11461 gggcgtatat aacagcaaca ttaggtgtat ctgcactaac atatggtcca tacgctttat
    11521 taaatattat aaatccaata gtatctttag tattaattgc atgtaagttt aaaatagcaa
    11581 gaatagaaga tgaacctgaa acttgtttaa attttgatgt ttgataatag ataacttatg
    11641 taagaacgta ggaactaggg gggtagcctg tggggaatta gtaagacata ttttagtgtg
    11701 cgaaattcta taaatcagta atcttgtgac tttagccatg ggaggttcag attagtaaat
    11761 ttatagtttt atattagtct atattttgat cttttaattg tttgtataaa ttttttatag
    11821 aaaaagattt cttttagact tgcctaaaaa ttaggcaagt cttttaagta tgttctgatt
    11881 ataagagcac tgtgtgacaa gaatgatgag attatttttg ctatactttc gatataagaa
    11941 atggaaggag gttatgtttt atattcatat gaaataatta gttacacagt aaattttcgt
    12001 ataatttggt ataaaaagat tagaaatgaa aataaagtaa tggaggaata aaatgactag
    12061 aatatccaat ataacaatag ctgaacaatc tgagtactgt tttttggcaa tacgaaaaac
    12121 aattgacttt atggtagagt tttcagagtt ttcaaaacaa agttttgaaa aaatttctaa
    12181 atatctggag gagagaggaa ttttatcttc aggagcaccg attgtatgtt ttcataacat
    12241 ggatttagca aaattggatg ttgaagtagg gtttcctgta gccacttata tagatgggaa
    12301 aaatgagatt ctacaaagga ttgttcctgc acagaaaata ataactgcaa ttgatttagg
    12361 accttatgaa ttacaagacc ctactttaga agacttattt tcatgggcaa atagcaatgg
    12421 atataaactt catggcgata tatattacca gtatttaaat gatatcaatc gtccagctag
    12481 tgaatatctt acgaaaatga tgttacccat tatatagaaa gttaaagatt atcgtttgtt
    12541 agttgataat tagaagaaca tttactgcta ctacataaat tttatcctaa ataatatcaa
    12601 ggtgatttaa tttatgtatt ctaatgtaac cccaatatat tgttaaggtc tacttaagat
    12661 gctcaaaagg ctatgcttaa tttattgtta tataatttgt agagtggtat aatttagagg
    12721 taatattata tagatatata gggggaaaat atgaaaatat atgatatatt aacacgtttt
    12781 tatattcaag atatagaaaa agccatacct ttttatgaaa atcttttaaa agaaaaatgt
    12841 tctttgcgtt tttcctataa agaagttggg ttggaattag cgcaaatagg aaatgtttta
    12901 ctattatctg gttctgatga tgctcttaaa ccatttatag aaacaaaatc tacttttatg
    12961 gttgattctg ttgatgaatg gagaagttat ctattggata atggagcagt tgtagtaaga
    13021 gataagaaaa aagttcctac aggatataat atgacattga gacatccaga tggtacaatt
    13081 atagaatatg ttcaacatac aaagcaggat tagttaaaaa cagattttac tactgagcaa
    13141 ctaattagaa tctattctta gacgtacaat ttattatttt tggtattaac agattataag
    13201 tcaaattata atatttcaaa atagaataga aatttattta cataaaagag agtgtttaaa
    13261 aatgaacttt ttagttcatg aagcactctt ttttgtatga aatcaaatac atcaatatat
    13321 aaaataaact gatgaagagc ttaaattaaa ataaaaagat tcataacagg accccattaa
    13381 actttaagat agggtaagtt tgtaatcata ttaaataatt gtaaaaagct gtttttagtc
    13441 gtataatata aatatttatc atttaattaa ggaatataaa aggaatctaa taagaagaaa
    13501 ataataaata actttttaga tttactaaat gaaaagttat tgaaaattaa aggattagag
    13561 tctttaatca gaaagtaaaa agtaatatat taaatttatg aattgaaaac ttaaacaaca
    13621 aatttgttta taaaatagca agattcatta aatataacta agtaataatt gatataatgt
    13681 tgcatggata aaaggaggga agctgcaatg tcttacaaag taattaatgt cataactgct
    13741 attgactata tagaggagca tttatcagaa aagctggatt tagatatagt tgctaatgct
    13801 gttcattact caaaatatca cctgcataga actttcacaa cttctgttgg tctaacaatg
    13861 catgattaca taaagcggag aaagttgaca gaagcagcaa aattattggt attttcaaag
    13921 aaaccaatta ttgaaattgc attgatagct ggatatgaaa gtcaacaggc attcacttct
    13981 atatttaaag ctatgtataa aaaatctcca aataaatata ggaaagaaca agaattttat
    14041 cctttgcagc taagatttgt tttgaaaaaa gataacctat tttcaaagaa tgtactagag
    14101 ttggaaaaag aaattaaact tgcaagtatg tctgacatac cattatggat gaatttagtg
    14161 agattagtca tagatggatt tccaaatttg caagaggaag aatatatata tcaattagaa
    14221 cagtatattt tagaagaaag agctttaata ttaaaactta ataatgtcgc tattgcaaat
    14281 atggtgttta atagagaaac tagaagtatt gatttttttg gaatacatcc acagtatcgt
    14341 aattcagaca ttgcacaagt gtttttgaag aaagttattg aagatttctt gatagataca
    14401 gatatttcaa ttactacctt tcgagagggt gacaaagctg atacaggaca tagagatatg
    14461 ataaagaagt tgggctttgc agaagcagaa ttgcttgtgg aatttggata tcctactcaa
    14521 aaatttattc ttccttgtgt agaagaaaga gataatagac aaaattaatt gtagattaaa
    14581 tgaaaagttt accacatatt ttttgtttga attattttat atataagagc atttctgctt
    14641 ttaatgtatt ttgcttgtaa gtgtacttat ggtaataaat atagaaagat ttagtttttt
    14701 aaaaaaggaa ctttaaagct aaaaagctaa catttaagtt aaatagcaag aatgattaaa
    14761 aactccaaaa cacaattaag tataatgatt cagtgtaagt acttaattaa caaaggagga
    14821 tttaaatgtc tgaaaataaa caagtagtaa attgcgatat tattgcatct aattctaaac
    14881 ataatatgtg tgaatatgat gcagagttat taactttttt tccagatgag attgcacatc
    14941 taatagagat aaataacaag ctagacaatg cttttaagaa agctgaaaat cttgttgaca
    15001 agcttgataa ggattatatg gacgctaaaa tgtatatggt aaaaaatcgt ggtgaaatag
    15061 acccacatga aatgtttcaa aatgaacaag gactaaaaca aatagataac tatggggctt
    15121 ttatggtaaa agttcgagat aagattaata aaatcaaaga ttctccatac tttgctagaa
    15181 tagattttag gctgaaagat atggatgatg aatctaagta ttatattggt agatttgcat
    15241 ttgattatga agatgagctt ataatacttg actggcgttc tccaattgcc agtatgtttt
    15301 atgattatga aatcggaaaa gctggatatg atgctccaat aggatgggtg gatggagaaa
    15361 taacaagaaa acgacaattt aaaattaaaa atggaaaatt agaatatgca ctagaaagtt
    15421 ccattaatat acaagatgat attcttcaaa aagaacttag tcacacttca gatgaaaaaa
    15481 tgaagtcaat catttcaaca atacaaaagg aacaaaatca aattattaga aatgataaag
    15541 ctgatacact gattattcaa ggggtagctg gttctgggaa aacatctata gcattacaca
    15601 gaattgcttt tcttctttat cgatttaaag ataaaatatc agcaaataat gtaattatcc
    15661 tatcacctaa taaagtcttt ggagactata tttccaatgt ccttcctgag cttggagaag
    15721 agccattatg tgaattaagt tttgaaaata tagcagaggt acaattagat agagttatta
    15781 attttgaaag tgagaaagac ccattagaaa taaatgatgc taaatggtct gaaagagtgc
    15841 gttttaaatc tacacttgat tttgtaaaac ttattgatga ttatataaaa caaatgccaa
    15901 ataagatttt tattccaaaa gactacactt ttggaagttt tacagctaaa agtgactgga
    15961 tacagagtag atttgaggca tataacagat atccagttaa gaagagatta gagaaggtag
    16021 ctgaagacat tcattataaa tttgaatcag ataatattat ggaagaagat ttaccaaagg
    16081 taaaaagtat acttaaaagt ttgaatggaa tgttgacaat aaaaaatact cttacactat
    16141 ataaggattt ctttaaacaa atgaatatat caaacatgtt tgtgatggca acaaaaaaaa
    16201 ctcttgaatg gtcagatgta tacccattta tatatattca tgctgcatat gaaggtatac
    16261 aagaagataa gattataaga catgttgtta ttgatgaaat gcaggattac acacctattc
    16321 aatatgctgt aataaactta ctttttaagt gtaaaaagac tattcttgga gattttggac
    16381 aattggttaa tcctaatcac actcatacac tagatgatat gaaacagcta tataatgatg
    16441 gggaactagt tatgttgaat aagagttacc gttctacttt tgagattatt aatttcgcca
    16501 aaaagattca agacatatct tcattagagc ctattgaacg acatggtgaa gaaccagctt
    16561 tattaaaatg taataacgag caagatgaaa taaataagat aaaaatagaa attgaagaat
    16621 ttaaaaagag tgataatgcg actcttggta ttatattaaa gacagataat gacgctaaag
    16681 ctgtatatga tgcgttaaga gaaaaatata gtgttcactt aatatcttct gaaagttcta
    16741 gctttacaaa aggagtatcc ataacttcta ttaaaatgtc aaaaggttta gagtttgatg
    16801 aagttattgt accttcagta aataataaaa catattatag tgattatgac cgcagtttgt
    16861 tatatattgc ttgtacacga gctatgcata aacttaagtt gacatatact ggtgaattga
    16921 ctcaacttat agatatgtag attatatact tattatgtaa gtgtttactt atataaatat
    16981 aacctgtcaa taaaattcat taataaatca ataaaaacaa tatttgtaaa agtaaaagct
    17041 ataaattata tattaaattt tataatttat agcttttata atactgctta tcttaatcct
    17101 cccaatagca aaataatttt aaacatgcca tattttataa atgtaataac aattatacaa
    17161 ttaaaataac tttcccaaac taacttgaca tttaaaactt actattgtaa tatttaaata
    17221 ttacaatagt aagttttaaa tgtggaatca attattatat cacaaattaa aaggaggaca
    17281 ggatgttatt aatagttact taattatctt cgcaataggt ttaatcaaca taattgtact
    17341 aggtgaagaa aatataagag atacagctat tcatttagtc ttgtttctta taagtagaac
    17401 ctagattgga gtatattaca aataatgtct actattttaa tagtaggtta taaatttaaa
    17461 tatgggagat gatataaatt atgaaaagaa aaaagaattt tatttggata gctatcttat
    17521 tggttggtgt agttatggta atgtattatg tagggaaaaa acataacgat ataaatcaaa
    17581 aaactgataa gaattatata aaatctgaat taaataagtc tgaattaaat aaatccaaaa
    17641 ttgaaaacaa taataaaaac aaaaaagaaa aaattaatat ggttgactat agtgattgtt
    17701 ttgaaggtat tagtggagga gctatttttt ataatactaa aaataaagaa tataatatat
    17761 ataacaaaga attaattgaa acaagaaggt caccatgttc tacttttaaa atagtttcaa
    17821 ctttaattgg tttagaaaaa ggggtaataa actcaaaaga atcagttatg ggatatgatg
    17881 gtacagagta tccaaataaa aattggaata aaaatcttag tttagaagag gcatttaagg
    17941 agtcttgtgt ttggtactac aaaaaattga tagataaagt tgatgctaaa tctgtccaaa
    18001 acattttaga tgatttaaag tatgggaatt gcgatattag cgaatgggaa ggagatttaa
    18061 aaaatggaaa gggacattta aatggatttt ggttagaatc ttcattacaa atatctccaa
    18121 aagaacaagt gcaaacaatg gcaaaaatat ttgagggaga tacaaacttt aaaaaagagc
    18181 atattaatat tttaagagat attatgaaaa tagatgtaaa tgataaaaac ataaatgtat
    18241 atggaaaaac tggaacagga tttgatgaaa aaaataaacg tgtggatgca tggtttgttg
    18301 gtatgctaga gcgtgaaggt gacacctatt attttgctat taaatcagat gattccaata
    18361 aagaaattac agggccaaag gtaaaggaaa ttgcaattaa tataattaag aaatattata
    18421 gtgtaaggga ataaacttaa ttttatttgt
//
LOCUS       DAYAOX010000063        18436 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.063, whole genome shotgun sequence.
ACCESSION   DAYAOX010000063 DAYAOX010000000
VERSION     DAYAOX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.063"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            153..521
                     /locus_tag="ACGI57_001704"
     CDS             153..521
                     /locus_tag="ACGI57_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFG9716784.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            524..895
                     /locus_tag="ACGI57_001705"
     CDS             524..895
                     /locus_tag="ACGI57_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HFG9716785.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1040..1495)
                     /locus_tag="ACGI57_001706"
     CDS             complement(1040..1495)
                     /locus_tag="ACGI57_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716786.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1534..2799)
                     /locus_tag="ACGI57_001707"
     CDS             complement(1534..2799)
                     /locus_tag="ACGI57_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HFG9716787.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
     gene            3269..4720
                     /locus_tag="ACGI57_001708"
     CDS             3269..4720
                     /locus_tag="ACGI57_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFG9716788.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(4939..5400)
                     /gene="ribE"
                     /locus_tag="ACGI57_001709"
     CDS             complement(4939..5400)
                     /gene="ribE"
                     /locus_tag="ACGI57_001709"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFG9716789.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(5505..6698)
                     /locus_tag="ACGI57_001710"
     CDS             complement(5505..6698)
                     /locus_tag="ACGI57_001710"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFG9716790.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(6721..7386)
                     /locus_tag="ACGI57_001711"
     CDS             complement(6721..7386)
                     /locus_tag="ACGI57_001711"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFG9716791.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(7420..8532)
                     /gene="ribD"
                     /locus_tag="ACGI57_001712"
     CDS             complement(7420..8532)
                     /gene="ribD"
                     /locus_tag="ACGI57_001712"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFG9716792.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(8664..8785)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            8974..11457
                     /gene="recQ"
                     /locus_tag="ACGI57_001713"
     CDS             8974..11457
                     /gene="recQ"
                     /locus_tag="ACGI57_001713"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFG9716793.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      11546..11657
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11799..13100
                     /gene="thiC"
                     /locus_tag="ACGI57_001714"
     CDS             11799..13100
                     /gene="thiC"
                     /locus_tag="ACGI57_001714"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFG9716794.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            13154..13348
                     /gene="thiS"
                     /locus_tag="ACGI57_001715"
     CDS             13154..13348
                     /gene="thiS"
                     /locus_tag="ACGI57_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFG9716795.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            13341..14144
                     /gene="thiF"
                     /locus_tag="ACGI57_001716"
     CDS             13341..14144
                     /gene="thiF"
                     /locus_tag="ACGI57_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HFG9716796.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            14207..14977
                     /locus_tag="ACGI57_001717"
     CDS             14207..14977
                     /locus_tag="ACGI57_001717"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFG9716797.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            14994..16097
                     /gene="thiH"
                     /locus_tag="ACGI57_001718"
     CDS             14994..16097
                     /gene="thiH"
                     /locus_tag="ACGI57_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFG9716798.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            16098..16787
                     /locus_tag="ACGI57_001719"
     CDS             16098..16787
                     /locus_tag="ACGI57_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFG9716799.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            16975..18369
                     /locus_tag="ACGI57_001720"
     CDS             16975..18369
                     /locus_tag="ACGI57_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFG9716800.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT         6856 a         2252 c         3029 g         6299 t
ORIGIN      
        1 tgtaattact tttattaata aaactcaaag ttatattagt aaaaattgga attatttata
       61 atatactcca aaatttaaaa ataatggttg aaaaatctaa ttaagagtac tataatttaa
      121 ttaagtaatt acttaatcaa aatgaggtac ttatggaaaa cttggttaaa atatttaaag
      181 cattgagtga tgaaactaga cttaacatac ttatattagt atccaagaga aatatatgtc
      241 aaaaggggat ttctaaatat ctagggattt cagattctgc tgtttcacaa catataaaaa
      301 tattaaaaga tgttgggata ataacaggat ataaagaagg atactatgtt ttatatcata
      361 ttaataaaga atcatttaat atatgtgtag attttataaa ttctatgtta agtaattcta
      421 gtgaaagctt tatagatgta tttgatgtaa atacaataca tctaggatgc agtaaagaat
      481 gtaaatctat aaaaaaatgt tgtaagagaa gggagaaata ataatgaaag tatgtatacc
      541 agtagaggag aataaaggat tggatagtaa accatatgga cattttggtt ctgctccaat
      601 ttttgttgtg tgtgatttag aaagtggaga agttaaatct ttagataatg gagatttaga
      661 tcatgaacat ggaaaatgtc aaccattaaa agcattgtca gggacagctg tagatgcagt
      721 agttgttgga ggaataggac aaggagctat aattaaatta aatggaatgg gaataaaagt
      781 ttacagagca gaaggtgata ctataagtgc taatttagac ttacttaaaa atggaaaatt
      841 agtagaattt ccatcagacc atacttgctc acatgacgga tgtggacatc attaaaaaaa
      901 aacagctgat gaaatatata cttcatcagc tattttttct tttaataaat acgattttat
      961 tcttttaaaa ccaaaattta gatttttatg ttttttgtag tatccttaat aatatttcaa
     1021 gtttacaagt ttactacttt tagatttttt ttgtatgttt ttctatatag tcaatatcat
     1081 ttaatatgtc attgagttct ttattaagtg tttttaaatc atcaccatct acattgtagt
     1141 ttaaaaatag ttcctctaca ttatcaatga tagaattgac ttttctagta acttcaacaa
     1201 atgtcttaaa gttactacta tttttagcat ttagaaattt ttttaaatcc tttgatataa
     1261 atgatactat ttcatattca tcattaaata gacctctacc aataggatat ggttcaattc
     1321 tctgtaaaaa atattggtct ttagcattaa tattataagt ataagatatt tcccaaccgt
     1381 ctcctaaatc aaacttgata aaatataatg tactacttac ttttgatatt cttgtggcac
     1441 caagctgtct taaaagaact tcaatcgaat caccttgaac agtattaata ttcatatata
     1501 cacatcccct ttagtattat tatactatat tttttaaaat tttataatta aataaacagt
     1561 agatataata atagatataa tcataagtgg aaaacctatt ttgaaatact ccttaaaaga
     1621 gataggatga ccgtgtttat tacctatacc tgctagtaca acattagcag atgctcctat
     1681 tagtgttcca tttcctccaa gacaagctcc aagagatgtt gcccaccata gtggcattac
     1741 atcaatacct tcagcttgca tagtcaaaat aagaggaatt agtgttgcaa caaatggaat
     1801 attatctaag aaagaagaga ctatagctga caaccataat atcaacaaca ttgtaaatac
     1861 aagatgtcct tctgtaagtc caatcaatgc ttgagccaat ttgtttatta tcccaacttc
     1921 tactaatcca ccaactacta caaataatcc cataaagaat aaaattgtag accattcaat
     1981 acctgccata atttcatcta catcttgttt tcctattaaa agcattatag atgctcctgt
     2041 cagtgctaca acagaagaat ctatgtggat tgtactgtga aacataaaac caaaaagtat
     2101 gaatgctatt aatataaggc tttttataag tagaggttta tctttaacag attttttttc
     2161 atctagtttt aatattgcat ttttagctct ttcattcaca acaagttctt ttccataaat
     2221 aaatctaaag catattatag ttatagcaag tattactata acagcaggac ctaaatttat
     2281 tacaaaatcc ataaaactaa gattggcagc actaccaatc ataatatttg gagggtctcc
     2341 tataagagta gctgttcctc ctatatttga agctaaaatc tgtgttatta aaaatggaac
     2401 tggattaagt cctaatattt gtgtaataac tatagtcatt ggtccaataa gtaagacggt
     2461 tgtaacatta tctaatacag ctgataaaat agctgttata atagcaaaac aaatcattat
     2521 cttccatggg tcacctttgg ctttctttgc agtccagata gctatatatt caaataaccc
     2581 cgagtttttg acaacagcta caaatagcat cataccaact aatacaccaa tagtattgaa
     2641 atctatatgg cttaatcctt catcaagtgt aagtatattg aatattatca ttaatagtgc
     2701 accaccaaca gctgctacgg atctgtttac cttttctgta ataatagctg ccataactat
     2761 taaaaatata catatagcta ttatttgttg aaattccata atataagtac ctccatttgt
     2821 attattttgt gcctaatgct agtactatat atagtattac ttttttattg ttttttcaat
     2881 ggatgtatga aagtatatta tttaacaaag tttttggaaa attgacagaa tttaaagaaa
     2941 atttttgtta tttaaataaa ttttaaaatt attccattga taataatatt aaacgacaaa
     3001 cgaaaaaata taccctttaa actagaattt attgtatcta ataacaatta taaaatatta
     3061 aatatatcta tattataaaa acaataaaaa aaggtttgat ttgaatataa aactgcaata
     3121 tattaaaaaa attattcatt ttaaaccttg taatttgatt ttataaatat tataattaaa
     3181 ttaatgaatt tttaacaagg aattattcat ttttttgatt ttaagattta ttgagaaaac
     3241 aaaactaaat aggggggtaa ttaatataat ggattttttc aatggtattt taacgagtat
     3301 taacaatgca gtagtatatg taaacaatta cttatggtcg tatatcttaa ttataatgtt
     3361 agttgtaatt ggtatttact ttactattaa aacaaatttt gtacaattca gatattttat
     3421 agaaatgttt agacttctgg gtgatggtac agcgaataaa aatgcaaaaa aggaaggaaa
     3481 aatctcttca tttcaagcat tttgtataag tacatcttca agagttggaa ctggaaatat
     3541 agctggaata gcaattgcag tggtagcagg aggacctggt gctgtatttt ggatgtggct
     3601 tatagcatta attggttctg catcaagctt tgttgagagt acattagctc agatttacaa
     3661 agtgaaagat ggtcaagctt ttagaggtgg accagcgtat tatatggaac aaggtctaaa
     3721 taaaaaatgg atgggtattt tattctcaat acttataact atatgttatg gttttgtatt
     3781 taatgcagtt caagcaaata ctgtttcact tgcatttaat aatgcatttg gaataagtaa
     3841 aatggctatg ggaataggtc ttggaatttt aactgcaata gttatttttg gtggagtgca
     3901 tagagttgca aaagtttctg aaataatagt tcctgtattt gcaggattat atatattagt
     3961 tgctttggct atagttgtaa tgaatataac agagatacca agtgttatag cattgatagt
     4021 tgaaagtgca tttgatttta aaggaatggc gattggtaca tttatgggag ttgtaatgac
     4081 tggagtgaag agaggtctat tttctaatga agcaggtatg ggtagtgctc ctaatgctgc
     4141 tgcaacagca catgtaactc atcctgtaaa acaaggttta attcaatctt taggtgtttt
     4201 tacagatact ataataatat gtagttgtac agcattcatg gtattattat attcaggtta
     4261 tagtagtagt ggagcaactg gaattgaatt agctcaagaa gctttaactc atcatatagg
     4321 accagttgga aatatattta tagcagtttg tatattcttg tttgcattta gttctatagt
     4381 tggtaattac tattatggtg aatcaaatat ggagtttatg agtggaagta agactaaact
     4441 taatgtattt agagtttttg ttgtaggtat ggttttattt ggttcactta ctcaagtgga
     4501 tgtagtttgg aatcttgcag acttatttat ggcattgatg gcgataataa acttaatagc
     4561 aatagcactt ttaggaaaat atgcatttat tgctttaaaa gattatacaa gtcaaaaaaa
     4621 atcaggaata aaagaccctg tgtttattgc agatgaaata gaaggcctag aaaaggtatc
     4681 agagtggcat agggaattat ctgaggaaca tttaggataa aaaatagtat aaaattataa
     4741 aaccattttg aaagactaga gtatgtatat actttatatg aatataatat tgaattaatt
     4801 tatattactg gaatttttaa ttccagtaat atttttttat tatataattt agctttatta
     4861 aaagttttta ttattaaaaa tagcttattt atcgtaaagt gaatgatatt gggttttaat
     4921 tattatgaat gttagtattt aatttaaaga cttaaataaa tttgccattt ctagcgcatt
     4981 catagcacaa tcataacctt tatttccagc ttttgtacct gctctttcaa tagcttgttc
     5041 aatattttct gtagttacga ttccaaatat aacaggtaat tcactgtcta aagatacttt
     5101 ggctatccct ttagagactt cactacatac ataatcataa tgagaagtag cccctcttat
     5161 aacacagcct aaacagatta tagcatcata cttaccactt ttagccattt tttgagcaac
     5221 aagaggaatc tcaaaagctc caggtaccca aactatttct atattttctg gagaaacgtc
     5281 atgtcttaaa agacagtctt cagctcctga taaaagtttt gatgttataa attcattaaa
     5341 acgagaattt attataccta tttttaaatc ttttccaata agttttcctt catatatcat
     5401 ttttacttcc tccaatttta tttatgttat agatttatat ttataaatat ctttaattta
     5461 attataaaaa actacttagt agaaaaatat catcatataa tcatttatat aatatctaaa
     5521 agatgtccca ttttatcttt tttagttttt aaatatagct tattaaagtc attaatttct
     5581 tcattaatag ggattctatt ttcgattttt attccatatt tctctaattg atttatttta
     5641 tctgggttat tacttaaaag atttatactt tttatgttta aattttttaa tatttgagca
     5701 gccatataga aatctctcat atcttcttca aatccaagca ttaaatttgc ttcaacagta
     5761 tcgtaacctt cttcttgaag tttataagcc ttaattttat ttaaaagacc aatacctcta
     5821 ccttcttgct tcatataaat taaaactcca cttccatttt ccacaatttt cttcatagaa
     5881 gattctagct gtaatccaca atcacatttt agagagtgga acacatctcc tgttaaacat
     5941 tcagaatgaa gtctaactag agtattttct atatttatat ttccataagt aagggcaatg
     6001 tgttcttcat ttgaataagt atctctatat cctatgattt caaaatttcc atattttgtc
     6061 ggaaggaaag cactagatac tttttctata gtagtttcat ttattttgcg gtattctata
     6121 aggtctttta tagtgatgat ttttaaatta tgcttttctt tgaaaatcat taaatcatca
     6181 actcttgcca ttgtaccatc atctttcatt atttcacaga taagaccaat ctctttaaaa
     6241 cctgcaagtc tagcaaggtc tactgaggct tctgtatgtc catccctgac aagaacccca
     6301 ccagtttttg ctattaaagg aaagatgtga ccaggcgttt tgaaatcttt acttgtagaa
     6361 ttttcatcac agaataattt tacagtttca gctctttcat atgcagatat accagttaga
     6421 gtatctgctc catctatagt aacagtaaag gctgtttgaa atgtatctgt attttctcta
     6481 accataagag gtatattaag acttttaaat tttttttcag tggcaggtag acaaattaga
     6541 cctctaccat atgtagccat aaaatttatt gattcaggag tagccatttc agctgccatc
     6601 aataaatctc cttcattctc tctactttca tcatcaacca cgattaccat tttcccattt
     6661 tttatatctt caatagcttc ttcaatagta ttaaacatgt aattcactcc tttattattt
     6721 ttaaaaacca tttttaaata aaaaatcttt atctatattt gatttatggt tattactatt
     6781 agaatttaat tccttgtagt tattagccat aaaattatta acatactttc caattacatc
     6841 gctttctaaa ttaacgaaat ctccaacatt ctttgtcaag agaatagtgt taattttagt
     6901 atgaggtata atagaaacct taaatatttc atcatctaaa tatgatatag ttaaacttac
     6961 accatcaata gcaacagaac ctttgagtat cattgaggat aaaagttttt tatcaggtcg
     7021 tatacttact aaaactgcat tttcatcttt ttcaaatttg cagatagttc ctttaccatc
     7081 aacatgacct gttactatat gaccaccaaa tcttgaagaa agactcatag ctctttcaag
     7141 atttaccttg tcatttgcat ttaataaacc taaatttgtg gaacgaattg tctccatcat
     7201 aacatcggct gtaaattcat tttttccgag gtttgaaaca gttagacaaa ctccatttac
     7261 ggctatacta tctccaagat ttgtgccatc taatatttta tttgatagta tggttacttt
     7321 tccactctta ccattagcag taatttttct aagtattcca acttcttcta ctatgccagt
     7381 aaacatccta acctcctata tgaatgtatt aacaaaaatt taatttaaaa catctgattc
     7441 tataagaata tcgccatcta tcaaagttac attattgatt gttaactgtg ttgaatctga
     7501 caaatgattt attccttctc cagaaacgaa tgtaggtgca tctttaccac ctataatctt
     7561 gggtgataga aataatttga ccttatcaac taacttattt ctaaagagac tgtcattgag
     7621 agtagcacca ccttcaacta gaacagaatc aatgtttaac tctcctaatt tatacattac
     7681 aaactccaaa tctactaagt tgttttttga aggagaaatt attgtttcaa cattattttc
     7741 ttttaacata gataatttta tactttcttt gcaacatgtg aaaactatag ttttactggt
     7801 tttatcttta accaaattac tattaagtgg caactttagg tgtgtatcta ttactattct
     7861 tgtaggatga gacacttttt cttgattgag cctacaagtt agtaaaggat tatcgttcaa
     7921 aactgtattt atgccaacca ttatactttg gtatttattt ctgtatttat gagttagata
     7981 ccttgatgat tcattagata tccattttga ttctagtgat tttgtagcaa ttttaccatc
     8041 tagacttact gctgatttta caatacaaag aggtcttttg ttttttatat gataaaaaaa
     8101 tgcctcattt aaatctcttg cttcatcttc taatattccc acagtaacat caatattgtt
     8161 atcttttaat ttttttactc cattaccaca aacaagtgga tttgggtcta atgtagatat
     8221 tacaactctc ttaatcttat tttgtataat tttatctaca caaggaggcg ttttcccata
     8281 atgagaacat ggttctaagt ttacataaag agtagagtct tttagacttt gtttagcact
     8341 gtttatagca tttacttcag catgattaga gccaaatttc tcatgatatc ctttgcctat
     8401 tatattacta gctttaacaa ttacacaccc aactaatgga tttggattta caaagccttc
     8461 tccattttta gccaattcaa tagctttttt catataataa atatcttttt ctttttgatt
     8521 cacaaaattc acaatatgta cacctcctgt ttaattggat aatttttaaa caaaaaaccc
     8581 tgagaattaa ctcagggttt agaataatct acaataaatg tttaataaat gtagaacaaa
     8641 attgctctac aaaaacactc attaaatatc ttcttccatc cagactttac tgtcggcttt
     8701 ggaatttaac caaatcaacc aagttttacc ttggctcgcg ggctataccg ccgattggga
     8761 atttcaccct accccgaaga ttaagtatta aatttatatt tagaatgtat cataaattat
     8821 tttactttgc aaactatttt tttaaaaaat atatgttaat atataatata gaatgtctat
     8881 aaactttgaa ataccacgat tgtgaatata aaaataaaaa tttgaacata atcaggaaag
     8941 aagtctaatt aatttaaatt aggagagtaa tatatgaata aaaaactttt agaaatacta
     9001 tcaaaatact atggatatac aagttttaga aaaggtcaag aatctataat aaactctatc
     9061 ttaagcgaaa aagatgtgtt agctattatg ccaacaggag ggggaaaatc aatatgttat
     9121 caaattccag ctttgatttt agatggaatg acaatagtaa tttctccact tatatcactt
     9181 atgaaagacc aagtagatgc cttaaagact atgggaatta gtgctgcttt tataaatagt
     9241 tcattaagta gcaaagaatt taatgaaata ttaaataata ttagaaaaaa taattataaa
     9301 atactctata ttgctccaga gagattagat gcacaagagt ttattgagct aataaataat
     9361 aacaatgtaa gtcaagttgc gatagatgaa gcacattgtg tatctcaatg ggggcatgat
     9421 tttaggttga gttatagaag gatttctgat tttataaaaa atttgcctaa aagacctata
     9481 gttacagcat ttactgcaac agcttcagaa gaggtaagaa ctgatataat aaatttactg
     9541 tgtttagaaa atccagatta ttatataact ggatttgata gagagaattt atcaataaat
     9601 atagtaaaat catctagtaa aaataagtat attttagatt atattcaaaa tcataaaaat
     9661 gagtctggaa ttatatatgt tgcaactaga aaggaagttg aaaatatata taatggttta
     9721 tctaagagaa atatatctgt gtcaaaatat catgcaggtt tatctaaaaa tgaaagaagt
     9781 aaaaatcaag aagattttat aaatgataat gtggatataa tggtagcaac aaatgcattt
     9841 ggaatgggta ttgataaacc taatataaga tgggttatac attacaatat gcctcaaagt
     9901 atagaaaact actatcaaga aataggtaga gctggtaggg atggagaaaa aagtgagtgt
     9961 gtacttttat tttctccagg agatgtacat atacaaaaat atttaattga tattggagtt
    10021 gaaaatcctg aaagaaaatt atttcagcat aagaagcttc aatacatgat tgacttagtt
    10081 tacagtaatt catgctatag aaagacaata ttgagttatt ttggagaaaa ttatatagac
    10141 aattgcaata attgtagcaa ttgtttagta gagggtgaaa tagtagataa aactatagat
    10201 gcacaaaaag tcatttcatg tgtagctaga atgaaaagaa gttatggtgt tactactatt
    10261 gtagatgtgc ttagaggttc taagaataaa aagattttac agcttggatt aaatactttg
    10321 tctacatata acattatgag agattattct agtgaagact taaagaattt tgtaaataca
    10381 ttggtttctc atggtttttt agatttagtt gagactctag gcaatggaaa tagaggtagt
    10441 tttcctacta taagattaaa tgagatgtct atgaaagttt taaaaggtga agtgaaagta
    10501 gagtttaaag aaattgttat gacaaaatca ttggaagtag aggatgagtt atataatgct
    10561 ttaagagaac ttagacattc aattgcaagt gaagaaagga tagctccata tatggtattt
    10621 ggagatggta cacttagagc catgtctagt agttatccaa ttaataaaga agaaatgcta
    10681 gatatctctg gagtaggaga aattaaatat caaaaatatg gtagagattt tgaagcagta
    10741 attaaagaat atgtcgaaaa aaataatata aataaaaaaa tagatgaaaa aaatgtcgat
    10801 tcaaagaacg ttaatagtga tttcttagag gttaatacag ataaagtttt atatgaaaaa
    10861 ctgttagtca ttagagaaga atatgcaaca aaggaaaaat tacctgcata tatgatttta
    10921 gccaataagg ctttgaaaga aataagtgga agatatccac ttgatgaaga acaattaaaa
    10981 gatatttctg gcataggtgg agtaaaattt gaaaaatatg gagctagtat tttggcagag
    11041 gttaataaat atgtaaaaga aaataatatt aatgttattt gggagaaaaa gggcaaaagg
    11101 aagttaatat tagatggaga aagtagaaaa aataatgaaa tagcattaga tttgctaaat
    11161 caaggaaaag atatacaaag tatcagtcaa gaaatagaag tttcactgtc aaccattatt
    11221 ggatatgtgc atgactatgt aaaggatgga catagtatat catttgaatt aggactagaa
    11281 aatttttata atgaagataa aaagaagatg attttagaag tttgcgaaaa agttggctat
    11341 gataatttaa ataacataaa aagaaaactt ccagattcaa taaagtatga aaatataaga
    11401 gctgtgatac ttgactctta tcttgaaaac ttaaaaaata atattatagc agagtaattt
    11461 ttaacttaat aaaaaataaa atttgtatta ttgtgttgaa ctaataattt tgctgtgtta
    11521 tactgaattt gtaaaaataa tgaaaaatta gagctagggg cgctagaatt ggctagctga
    11581 gagataaagc cgtaacttac aaaccctaat aacctgatca agataatgct tgcgaaggga
    11641 agctttttaa tataatttta ttatataaat aggcaaatta ttcaatgtta ggagaaattt
    11701 gtctaagtgt atatacgatt ataagagtgc tttccaaacg aggaaaagca ctttttttat
    11761 tttcaaaaac tataacttta atcttggagg attgtaatat gaactataca acacaaatgg
    11821 atgctgctag aaaaggcata ataactaaag aaatggaaat cgtttctcaa aaagaacagg
    11881 tagatgtaaa tgaattaaga gaattaatag caaatggaca agttgttata ccagctaata
    11941 aaaatcataa atctttaagt gcagaaggtg taggtaaaaa tttaagaaca aaaataaatg
    12001 taaatctagg aatttcaaga gattgtaagg atatagaaaa agaacttgaa aaagttagag
    12061 tagcaataga catgaaagct gaagctataa tggatttaag taattatggc aaaactagag
    12121 aatttagaga aaaggttgtt gaaatgtcac ctgcaatgat aggttcagta cctatgtatg
    12181 atgcagtagg atatttggaa aaagaattaa aagatataac agaagaagag tttttaaatg
    12241 taataagaca acatgctatt gatggagtag actttattac tatacatgca ggtcttacaa
    12301 gaagtgtttg ccaaaaaata aaaaatcatg agagattgac tcatatagta tctcgtggag
    12361 gttctttatt gtttgcatgg atggaattaa ataataagga aaatcctatt tatactaatt
    12421 ttgataaaat attggatata tgtgaagaat atgatgttac attgagttta ggagatgctt
    12481 gtagaccagg ttgtataaaa gattctactg atggtgttca aattcaagaa cttgtagtat
    12541 taggtgaact tactaaaaga gcatgggaaa gaaatgtaca agtaatgata gaaggtcctg
    12601 gacacatggc tattgatgaa atagaagcaa atgtagtatt agaaaagaga ttatgccatg
    12661 gagcaccttt ttatgttctt ggaccattgg ttacagatat agcaccaggt tatgaccata
    12721 taacaagtgc aataggagga gctttagcat gtgccaaagg agtagatttt ttatgttatg
    12781 taactccagc agaacattta agattgccta atttagatga tatgaaagag ggtataatag
    12841 ctgcaaagat agctgctcat gcaggagata tagctaaaaa tgttaaaggt gctcgtgaat
    12901 gggataataa gatgagtaaa gctagagctg atttagactg gtgtgagatg tttagacttg
    12961 caatagaccc tgaaaaagct aagagataca gagatgaatc aactcctact catgaggata
    13021 gttgtactat gtgtggaaaa atgtgctcaa tgagaacagt taagaaaata ttaaacaatg
    13081 aggaacttaa tctaatataa tttttggcta gtatgactgg aataaaaata agctgaaaag
    13141 aggaggtgat ttagtgaagg ttaatgggaa ggaaattgaa tttgaaaaag atttgactgt
    13201 tatagattta ttaaataaat ataatttaaa aagtgataga gttgtagttg aagtgaattt
    13261 agagataatt gaagaatcta actacaatac atatgtatta aaagacgagg acattgtaga
    13321 gcttattagc tttattggag gtggttaaaa taaaaatatt tgtaaatgaa ttattaacag
    13381 atgttgagga aaatacaact gcttataaag ttagagatag ctataacaat aaatgtgatg
    13441 tattggtgtt aaatggatat ccaattaaaa cagatatgac cctttgtgaa aatgataaat
    13501 taacacttat acaaaaagga gttaagccat cacttgatga attggaaagc ttgttgattg
    13561 ctagacatac tccaaatata cataataaat taaaaaaagg gaaagttgct atattgggat
    13621 tggggggact tggttccaat atagctatct ctttagctag aataggagtg ggagaacttt
    13681 tacttataga ttatgatgtt gtagaaccat ctaatttaaa tagacagcag tattttatag
    13741 atgatatagg aaaactaaaa actgatgcca tgatagaaaa tataaaaaaa ataaatccat
    13801 ttataaaatt aaataaacga gatatttttt taaacaagaa caatatggat actattaagg
    13861 attcagattt aataattgag gcctttgatg atgcttcatg taaagcacag gtatgtaatt
    13921 atgtattaat aaatttaaaa gataaatatt taatagcatc atctggtatg gcaggttact
    13981 atgattcaaa tataataact actaagaaga taaaagacag gttttatata tgtggggatt
    14041 ttgtgaatga agctaaattt ggtgaagggc ttatggctcc aagggttgcc atctgcgcaa
    14101 atcatatggc aaatttggca acgcaaatac ttatagatat gtaaatatta aaattagata
    14161 taaaaaagat tgaatattaa aaaaatttaa cgtggaggga aataatatgg ataaattggt
    14221 acttggtgga catgaattta atagcagatt attggttgga acaggtaaat atggttcaaa
    14281 taatatattg cctgaggtaa taaaagaaag tggaagtgaa ataataacaa tggcacttag
    14341 aagagtggat ttagacaata aacaagaaaa catattaacg tatattccaa aagagatgac
    14401 aatacttcca aatacatcag gagctactaa tgcagaagaa gctgtaagaa tagctagaat
    14461 atctagaaaa atgggatgtg gagattttat aaaaatagaa gtaatatcag atactagata
    14521 tttacttcct gataatgaag aaactataaa agctacaaag atacttgctg atgaggggtt
    14581 tatagttctt ccatatatga caccagacct ttatgctgga agaagactta tagaagctaa
    14641 tgcagcggca gttatgccac ttggagctcc aattggttca aatagaggtc ttcaaatgaa
    14701 agaaatgata agaataatga tagatgagtt ggatatacca ataatagtag atgcaggtat
    14761 tggaaaacca tctcaagcta tggaggctat ggaaatggga gctgatgcag tacttgtcaa
    14821 cactgctata gcttcagcag gagaccctgt acaaatggct agagcattta aattagctgt
    14881 agaaggtgga agagaagcat atattgcaaa aacaggaaat gtgtcagaat ttgcaaatgc
    14941 atcttctcct ttaacaggtt ttttaggcaa tttatagaaa gtggtgattg gtgatgagtt
    15001 tttatgatgt aatagaaaaa tatagggatt ttgattttga tggatattta aataatgtta
    15061 ctgataatga tgttttaaga agtttatcaa aagacaagct tgaagatttt gatatattaa
    15121 atttgctttc taaaactgca gtaaaacatt tagaagatat ggcacaaaaa gcacataaat
    15181 taagtgttca atactttggt aaaacagtat gtttatatac accaatgtat atagcaaatt
    15241 attgtgttaa tcaatgtgtt tattgtagct ataatataaa gagtggaata aaaagaaaaa
    15301 aattaactat ggatgaaatt agagaagagg gagaagtaat ttcaaaagaa ggtttcaaac
    15361 atctacttgt tttgactgga gaaagtagtt ttcactcaag tgtagaatat ataggtgagg
    15421 ctatagaaat attaagagag aaatttccct ctataggtat agaagtttat ccaatggaag
    15481 tagaagaata taagtacatt gtagataaag gtgttgaagg attaacagta tatcaagaaa
    15541 cttatgacga ggaaatatat aagagagtac atataaaagg cccaaaatca aattataaat
    15601 ttagactaga tgctcctgaa agaggggcaa aagcaggtat gaggacttta tcaataggtg
    15661 cacttttagg gctaaatgat tttagaaaag aaactttttt tacaatattg catgggaaat
    15721 acttaaaaac gaaatatcct catattgagc tttcatattc aacaccaaga atgagacctt
    15781 ttaaagggtg ttttgaggaa ttggtagata taagtgatac tgatttagtt caagctatgg
    15841 tatgtatgag attatttgac cctcatgcag ctataaatat atccactaga gaaaatttag
    15901 aaatgagaag tcatataata cctttaggag taactaaatt gagtgcaggt gtatctactg
    15961 atgtaggagg tcattctcaa gatgaacatg atactgctca atttaagata aatgatgaaa
    16021 gtactgttaa agatgtggag aaaatgctaa actcaattgg ttatcagcat gtgtttaaag
    16081 attgggaaag attttaaatg tatctgataa caaacagaaa attatgtagt gaggaacgat
    16141 atttagaagt tattaaagag tctatattga gtggtgtaga aaatataata ataagagaaa
    16201 aggatttaga atatcaagag ctaagaaaat tatatatgaa aataaaaact aaaatcaatt
    16261 gtatagattt tcaagagcaa atatcagatg aaagtttgaa aactaatata aatcaaaaag
    16321 agtttagaaa taaattcaag gttaatttta taataaatag taacattgaa ttttttgaaa
    16381 aagtggattg tcaaggtatc catcttcctt ttaaattatt cttgaattta attgaaaata
    16441 agtataattt taatgaaaat aaaatactag gattatcttt acataaagta gaggaagtag
    16501 attatttaga aaaattaata agaaatcaaa atataaaaat agattacata acattatcac
    16561 atatatatga aactaaatgt aaagaaggat taaatccaaa aggaattgag cttttaaaag
    16621 aagctaaaaa gattactgat attaaaataa ttgcacttgg gggaatttta ccatctaatg
    16681 tcaaggaaac attaaaatac tgtgatgatt ttgctataat gtcaactata atgaggtcta
    16741 aagatattaa aaaaaccata tcaaattata atgaaaagtt gaattagtaa atatagtata
    16801 ttgaattttt aacttaaata aaaataaaaa agttatcttt taaattttaa aaggtagctt
    16861 ttttatttta tatctttgct aataaaaaat tgtaaaaagg gaaaacttta gtcagagaga
    16921 ttaaacaata tcctagaaag atttaaaatt tagtaaagta tggaggacat tataatgact
    16981 gacagtaaaa agaaacgatt tattatacca cacactttta caataatatt tttactaata
    17041 gtcttgatgg caatattgac ttggatagta ccaagtggtg aatttagtag agaagatgta
    17101 gaaggtagaa tggtagttgt accagggact tatcaaagtg tagaaagtaa tcctcaagga
    17161 tttgcagatg tttttaaagc accaatagaa ggctttatcg attcagctga agttgtaggt
    17221 tttgttttaa tagttggagg tgcttttggt attgtaaata ggactggagc tatagaagca
    17281 ggaatagctt cggttatctc taagtttaag ggaaaagaac ttcttataat tccaatatca
    17341 atgattttat ttggacttgg aggtacaaca tttgggatgg cagaagagac attaccattt
    17401 tatatgatat ttgttccact catgacatcc ttgggatatg attctctaac agcaatagca
    17461 attgtattta taggagcagc agcaggagca gccgcttcaa caataaatcc attttcagtt
    17521 ggtattgcac aggcactagc tcaacttgct cctggttcag gagtaactta cagagtagtt
    17581 atttttatag catatatact aataagtata gcatttgtta tgtactatgc taagaaggta
    17641 aaggaaaatc ctgaaagttc aatagtatat gaaatagata aaaataataa atcaatactt
    17701 aatcaaactt caacagaaat aaaagaattt acttttaaag aagtatctgt tcttgctata
    17761 tttggaatag ggatggcaat aatgatttgg ggtgttttaa gtcaaggatg gtacattcca
    17821 gaaatatcaa tgtgttttgc tgcaataggt gtgctttctg gattggttgg tagattatct
    17881 caaattgaaa tctcagaaag ttttatagat ggagcaaaag atttagcatc tgcagctctt
    17941 gctatagctt tggctcgtgg aatcgtaata attgctcaaa atggatttat aatagatact
    18001 atattaaatt ccgcagcagc ctttctaggt gggcttccaa aggttatatt tgtatattta
    18061 tcatttttct tggaagctgc attggcattt ttaattccat catcttcagg tcttgcttca
    18121 cttacagtac ctgtacttgc tcctcttggt gatttggtta atatatcggc tcaagtgata
    18181 gtaaccgctt atcaatttgg tgtgggtctt acaaacttta taactccaac ttcaggagta
    18241 ctgatgggag cacttgcagt agcccatatt ccattttcaa aatgggtaag atttataatg
    18301 cctatcttgt tgattttgac agctgtatct ctagtctttt taacaatagg tatttatata
    18361 gggttttaat taagtgtagt tttatttaag atatttagta atggtataaa atgataatat
    18421 gttttcaata aattgc
//
LOCUS       DAYAOX010000064        18319 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.064, whole genome shotgun sequence.
ACCESSION   DAYAOX010000064 DAYAOX010000000
VERSION     DAYAOX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18319
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.064"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            42..716
                     /locus_tag="ACGI57_001721"
     CDS             42..716
                     /locus_tag="ACGI57_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9716801.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            740..1744
                     /locus_tag="ACGI57_001722"
     CDS             740..1744
                     /locus_tag="ACGI57_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFG9716802.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            1856..3085
                     /locus_tag="ACGI57_001723"
     CDS             1856..3085
                     /locus_tag="ACGI57_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFG9716803.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALSSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            3093..3767
                     /locus_tag="ACGI57_001724"
     CDS             3093..3767
                     /locus_tag="ACGI57_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716804.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            3770..5002
                     /locus_tag="ACGI57_001725"
     CDS             3770..5002
                     /locus_tag="ACGI57_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9716805.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(5288..6553)
                     /locus_tag="ACGI57_001726"
     CDS             complement(5288..6553)
                     /locus_tag="ACGI57_001726"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFG9716806.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(6606..7754)
                     /locus_tag="ACGI57_001727"
     CDS             complement(6606..7754)
                     /locus_tag="ACGI57_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFG9716807.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(7845..8390)
                     /locus_tag="ACGI57_001728"
     CDS             complement(7845..8390)
                     /locus_tag="ACGI57_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HFG9716808.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(8613..9557)
                     /locus_tag="ACGI57_001729"
     CDS             complement(8613..9557)
                     /locus_tag="ACGI57_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9716809.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            9943..10824
                     /locus_tag="ACGI57_001730"
     CDS             9943..10824
                     /locus_tag="ACGI57_001730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9716810.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(10885..12093)
                     /gene="tyrS"
                     /locus_tag="ACGI57_001731"
     CDS             complement(10885..12093)
                     /gene="tyrS"
                     /locus_tag="ACGI57_001731"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFG9716811.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(12166..12418)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(12570..13538)
                     /locus_tag="ACGI57_001732"
     CDS             complement(12570..13538)
                     /locus_tag="ACGI57_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HFG9716812.1"
                     /translation="MNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDFIVPVFFC
                     IICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINKPTMVMDD
                     LVYGSVFGLIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLMIALFPFG
                     MVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICVSYLAIFE
                     VLKLLNVKVDSVYLKTIFISITYISLYFIIRDSCMSLQNFIIAKFRKLLYARILFLII
                     ILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            13711..14151
                     /locus_tag="ACGI57_001733"
     CDS             13711..14151
                     /locus_tag="ACGI57_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9716813.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKN
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            14478..14705
                     /locus_tag="ACGI57_001734"
     CDS             14478..14705
                     /locus_tag="ACGI57_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9716814.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            14731..16851
                     /gene="feoB"
                     /locus_tag="ACGI57_001735"
     CDS             14731..16851
                     /gene="feoB"
                     /locus_tag="ACGI57_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFG9716815.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKNSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(16984..17802)
                     /locus_tag="ACGI57_001736"
     CDS             complement(16984..17802)
                     /locus_tag="ACGI57_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9716816.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            18021..18293
                     /locus_tag="ACGI57_001737"
     CDS             18021..18293
                     /locus_tag="ACGI57_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716817.1"
                     /translation="MKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKAVKLE
                     IFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
BASE COUNT         6514 a         2733 c         2411 g         6661 t
ORIGIN      
        1 atagttatgt taatataact attttgaatt aggaggtaag aatgtttaaa ataatggtag
       61 ttgaagatga cgtctcattg aaaaatatca tagcaaagtg tcttactaaa tggggacatg
      121 atgttcatca aatagaaaat cttgaaaata taatagaaga gttcaaaaac tataacccag
      181 aattagtttt attggacata aaccttccat tttatgatgg ttttcattgg tgtaatgaaa
      241 taagaaaaat ttcaaaagtt cctataattt ttatttcttc aagaaattct aatatggatg
      301 taataatggg tgtaaattta ggtgcagatg attatataca aaaacctttt tctgtagatg
      361 tcttagtagc taaagttaat gcccttttaa gaagaactta taattttgtt gataataact
      421 caaatcagat tattcacaat ggtgttactt tagatttatc aactgcaaca ataaactatg
      481 aagataatac tattgagcta acaaaaaatg aaatcaaaat tcttcatgag cttatgaaat
      541 ataaggggca gatagtaagt aggaataaac tcatgaaaaa attatgggat aatgattggt
      601 ttgtagatga taatacatta actgtcaatg taaatagaat acgctcaaag ttaaatgaaa
      661 ttggtttaga agattttata gaaacaaaaa gaggattggg ttatataatc agttaaaaca
      721 attctaagta ggtgaattta tgagctttaa agattttctc aaggataaac taggtttttt
      781 agtatacaat tttactttct taatttttac agtatcagtt attatatttt ctcctgtaga
      841 tgtttttctg actgatacta ttttatatat attagttgta aatgttgtgt ttttattttt
      901 atatctattt attagctaca tcaagaagaa caaattttta gataagataa aaaatgatac
      961 attcaaaatt aatatgaatg atatagaatt agctagatgt aaaaatgaag aaaaaatata
     1021 cttgaacatt attaaaaatc atcaatatca atgtaataac ataataaata ataatatcga
     1081 gaaatttgaa gaaaacaaag agattttaaa tatgtgggta catgatgtaa agatacctat
     1141 agctataata aaactcattc ttgaacaaaa tgaaaattta attgatgaaa aaatttcaga
     1201 tgacatagat agtgaaatat ttagaattga aaactccgtt gaaaaaattt tatatttatc
     1261 aagactcgaa gattttcata aggatttttt aattcaagag gtaaatattg aaaaaatcat
     1321 tagaaatatt ataaaaaaat actcaaaata ttttatttca aaaaaaatta ttttagactt
     1381 aaataatttg aattttaagg ttttatctga tgaaaaatgg ctatacttca tattcgagca
     1441 attattgagt aattccctaa aatactcctc attaggagat aatatatcta tctattgcaa
     1501 aagaaccaaa aattatttac aattaagagt ggaagatact ggttgtggta taaaaaagga
     1561 agatttaaat cgaatattca ataagggatt tacaggtaat aatggaagaa acaattctaa
     1621 atctacaggt cttgggcttt atttagtaaa agaactttgt gataagcttg gtcataaaat
     1681 tgatgtagat tcagagtaca accaatatac aaaatttact attacattca aatgtgattt
     1741 atagtatctt attattgata gtttattttc attaacttat attcaacttc ttatagtaat
     1801 tctatatgta catcccagta tataattaag tagtttttat aaaaaggagg aagacatgtc
     1861 ttttctaaaa aaatttaaaa ataaaagatt tagaaataac agttttaaaa gtaacaactt
     1921 tagaaatagc agttttggaa gtagtggctt caagtctaaa aataaaggtt ttaaaaacaa
     1981 tccctttaaa aataagagat tcaaaatata tgcaggaata gcagtaatca tacttttaat
     2041 tctaggagtt cttgcttatg ttgattcgaa aaacactgaa ttacaagcta atgaaaattt
     2101 tatcgaaaca tatactattc ctgaaaatga aaaaatattt ataaatggga tggttgttcc
     2161 taagcaaact aaggacttca atatatctgg agattatgag ttaagtgatg ttaatgttac
     2221 aaatggtcaa aaagtaaatc aaggagacct tctttttact gctaaaaatc caactataat
     2281 agcagaaata gatagtttaa aatcacaact tagtcaatac aaaaaacaaa aaatatcact
     2341 ttctgatatt gctgaaaata aagatgctat tgcttctata aatgcacaga taactgcttt
     2401 aagctcccag atagcttctc tggataaaaa agcttatgac agagttactg ctccttttga
     2461 tggcacagta tacttgaatg accaaactgg aaatccagac caaccagttt catttatgac
     2521 tattcaaggt ctagagtttt atatgaaagg acaagcaagt gaacaagatt tacctaaaat
     2581 gaagattgac caaatggtca atatactagt tttttcaact gaccaaaagc ttactggtag
     2641 aataagtttt atatctgata aaccatctac tccaaatact gaaatgggag ctcagcagaa
     2701 tacactatct tattatgata taaacatagc atttgataat caagaaggat tagtaaatgg
     2761 attccatctt caagcatcac ttgaagtagc taattcaagc tttaaaatac cagcatcttg
     2821 tgtacttaaa gacaacaaac actcatatgt atttaaggat ttagatggta tattgaaaaa
     2881 gcaaattgta gatgtagcaa gtcaaaatga tgattttgct gtagttagag gcggtcttga
     2941 acaaggcgat ataataataa aacaccctac taaagaaatg aaagaaggag acccagttca
     3001 aggcgatggc gtaactgctg gttctaataa tggagatact actccaaaca aaaaagtaga
     3061 agaagttact atggatgtga attaatttac atatgttaat taaattagaa aatattcaaa
     3121 aatattacaa agtagggaaa gatgaattac atgttttaaa atccttaaat ttagaaattg
     3181 aatctggaga atttgtcatg ataatgggta agtctggtag tggtaaaact actttactta
     3241 atatcttagg ttttctagat gtatttgatg aaggaagata tatatttgat ggaacagatg
     3301 taactaattt gagtgaaaat gaacgttctg tttttagaaa tattaatatt ggatttgttt
     3361 tccaacaatt taatttaata gaaactctaa atgtatatca aaatgtagag ttgcctctaa
     3421 tttacaataa agcactaaaa aaatctaata gagaagaaat tgttaaagat aaattaagtt
     3481 ctgttgggct tttggacaag cttaagcaga aaccacttca attatctggt ggtcaacaac
     3541 agcgtgtagc cattgcacgt tgcctagcaa atgaccctca gataatcttt gctgacgaac
     3601 cgactggggc attagatagt gaaactagca gagaaattat ggagctttta acaaggctca
     3661 acaaacaagg taagactata ataatggtta cacatgatca agatttaact aagtatgcaa
     3721 ctaaggttat acgacttaaa gatggtgtgt ttacttcgga ggtgtaatca tgagtttatt
     3781 aaaaaattct ttggccaatt taaaaggtca caagcttcgt gtatttgtag ctttactctg
     3841 gattataata ggaataactt cagtaatact tgttagttct ataggtaatg gatttcaaaa
     3901 agaaattaag aaatctgtaa acaatgtaaa ccctaataaa actactattt cgtttgaatc
     3961 agccgataac acaggtctta ctgatgatat gagtattttt ttaaaaccat ttaatgcaaa
     4021 agatttagaa gaattatcat ttgttgaagg agtagaaaga atagctcctt caagagatgg
     4081 gtttaacctt gattcagttt actcttcaca agcatctttt gataaaaaaa ctacgtatgt
     4141 tgatgttgga ccagtaaaaa aagattctaa aataaattta atttgtggta gagatttttc
     4201 tttagatgat gaaaaaagaa aagtaatttt acttacatta caaagtacaa gtgaaatttt
     4261 tgaaaatcca gaagatgcct taggtcatgg tatcaacata aatggaacta tatttgaaat
     4321 aataggggta ttagatgatt ctcagcaaaa ccaagctgga ggattctttg gaggttacca
     4381 agatatgcaa tttactactt ccctagtacc taaaaaggct tttgacactt taatgagtca
     4441 aaattcttat tcaaatgaaa tttaccagtt agacttagtt tcttctaaag gctataatgt
     4501 aaatgaagtt gcaaacaatg ttatagctaa attatatgag atgcaccctg gtatcaatgg
     4561 ttcttatact acaccagacc caactgaaca gacagcttat cttgaaagca ttaattccaa
     4621 tgtaaacaaa tatgtatcta taataactgt agttgcaatg tttgttggtg gtattggtgt
     4681 aatgaatata atgtatgtat ctgttatgga aagacaaaga gaaattggta taagacgtgc
     4741 tattggagca aaacctaggt ctatattatt tcaattttta gttgaagctg tttttataac
     4801 agtatgtggt ggaattttag gaactatagt tggatttact gctactaact atgtttctaa
     4861 atatataggt tttgaagcta tacctagctt aaatagctta ttttatgcaa ttgtagctac
     4921 tatattgact ggagttgtat ttggtctgat tccagccttt aaagcctcta aactagaccc
     4981 tataaaggca atttataagt agaaattgta taaagatttt cgatttttag attagtttat
     5041 ataaaacata cttaaatata taacgcacta ttatatatga tagattagga acttaatctt
     5101 tccaaatata atagtgcgtt ctttcataaa aaggattctc ttccttacta tatctaaatt
     5161 accaagatgt tatgttctaa ttgttcaaac taatacttcc aattttgatt aatcaatctc
     5221 attctcctaa tatacaatct tttactttca atcattgtac actccgccta aaaaaaataa
     5281 tcttaaatta ggcgttatat acaatctccc cattacatat tgtataatta acctttccat
     5341 ataaagattt acctttaaat ggtgaattat cagctttaga aacaaagctt tcaactgtcc
     5401 attcttcttc tggattgaat ataacaatgt ctgctacagc cccttcttct ataaatccaa
     5461 tatttaaatt gtacaacttt gcaggattta tagacatttt ttctattaac tgcatcatac
     5521 taagatgatt tttatgcacc aaatttgtta ttccaagagc taaagaagtt tcaagcccaa
     5581 taattccact tggagctttt ataaactctc tatctttttc ttctttacta tgaggtgcat
     5641 ggtccgttgc tattatttca atagtaccat catttaatcc ctctataatc ttatctctgt
     5701 cccatttact tcttagaggt ggattcattt ttgcatttgt tccaaactcc aatacatctt
     5761 cctcagtcaa tgtaaagtga tgtggtgaag cttctgcaac tacatttgca cctaagtatt
     5821 ttgcaaatct aaccatatca acagatacac ctgaacttat atgttgtata tcaactttag
     5881 caccagtctt tagtgccaac atacaatctc tggctaccat aacatcttct gctacacttg
     5941 atgcaccttt taatccaagt tctgaagcta cctttcctgc atttacacct ggatttccaa
     6001 ctaaagaagg gtcttcttca tgaaaactaa gtggtacatc caactctttt gccttaatca
     6061 ttgcctccat aattagattg ctatccatta aaggtaatcc atcatcagta aaccctggta
     6121 caccagcttt tttaagagct tccatatcaa ctaactcttt accttcaaaa cctttagtta
     6181 tagctgctgc ttgtaaaaca tttatacatg cattttttga cttttcttta acataattaa
     6241 aagtatcttc attatctaca ataggattag tatttgccat acatataaca gttgtaaatc
     6301 cacctctagc agcagattta gctccacttt ctatatcttc tttatatgta aacccagggt
     6361 ctctaaagtg tacatgtaca tccactaaac ctggagcaac tacattacca ctagcatcta
     6421 ttattttttc atattcatca ctttcatcaa acttgcctat tttataaatt ttattttctt
     6481 ttataattaa gtcaatattc tcatctcttt gacttttagg gtctattact ctaccatttt
     6541 taattaaaat catattattt ctcctttata tttttaattt atatagactc atataatatt
     6601 atacattatt ttacatactt aaatacaaaa ttacgtttga catcaatagc ctttttagga
     6661 caaagttcat ggcaacagta acatcttata catttactta agtctataat agcaccctta
     6721 gtccctatct ctatagtgga tgcaggacaa gcttccttgc attttccaca ttttatgcaa
     6781 tcttgaaatc ttaccacagg ctttggtgtt ataagctttg aaataggttc atttaaaaat
     6841 tttggtagct ttacagaaaa caatcttaaa tccttactta catttggaat cttgtaattt
     6901 tttataatta aatcttttat atcttcacct aaaatttcaa tatctgaaaa atcttcttgt
     6961 atatatcctc tcttaatagc acctctaatt gtagcaactc tatttggaag taaacttata
     7021 atcttacatg caacaacatc tatagcatat ggattttgtg atgctaataa aacgcctatt
     7081 tctcgagtag taccagctga aggtccatca ccttccatac ctactatacc atccataatt
     7141 gtcaatgttg gttttatata atcacaaata tctaaaagta cattttcgca aaacaactct
     7201 tctgtaggaa atctataatg tatctctgct tttttcaatc cagctatgca tccatataag
     7261 ttttttgtac caccagtaaa agttgccatt atatgagtct ttaatttaca aagatttatt
     7321 atataatctg aatctactat tggtttaata atatccatat attttagaac atgtgctttt
     7381 tcactatgta tcttaacatc tgaaacatca taatttaact taatgcccag ttcatttgct
     7441 acttcttcaa ttccagatgc cttataaatg ctcttaagag aactttgatt atatggacca
     7501 ccagggctat ctgctattgt aatatcacaa tttaactcta aaagtttttc acatacaact
     7561 tttacaacca taggatgtgt agttgtagct tcttcaggct tttttttcat taataaattt
     7621 ggttttataa gtactttgct tcctttttta acaaacttat ctataccacc aatatcctct
     7681 aaattttttt caatagataa ctttacttct tcataattgt aagttttaca actcctaaca
     7741 gataccgact tcatattatc tccatccttc tatcacaaca gtttatttgt ataatttaaa
     7801 aaaggagaaa ccaaagtttc tccttttaat ctatgtttta attattattt tccaaaatgt
     7861 ctgtttaata atcctaagaa tgctttacca tgtctagctt cgtctttaca catttcatgt
     7921 actgtatcat gtatagcatc taatcctaat tctttagctc tcttagctaa ttttaatttt
     7981 ccatcagttg caccatactc agcatcaact ctaactctta agttttcttt tgtatctgca
     8041 actacaactt ctccaagaag ttctgcaaat ttagcagcat gttcagcctc ttcaaaagct
     8101 attctcttat acgcttcagc tacttctgga taaccttctc tatcagcttg tctactcatt
     8161 gctaaataca ttcctacttc tgtacactca ccagtaaagt tggctcttaa tccttcgatt
     8221 atttctgcat ctacaccttg agctactcct attctatgtt catcagccca aaccatttcg
     8281 cctttcattt cttcaaattt atcagctcca actttacata ctggacattg tgctggtgca
     8341 gcatctcctt catgtatata tccacatact gtacaaacaa attttttcat aattaatttc
     8401 ctcccaattt tatataatta aatttttatt tttaagttta ataacttgct acaagaatat
     8461 atatacccca gactttatag cttaaacact aaaaataaaa tttatttcct aattaatttt
     8521 atcactttga aacaccagta tcaaccaagt tttgatgtca aatttctaca taatacataa
     8581 tttatttgat taattaggaa atagttgtca tattaaatac tttctaatga agactcctct
     8641 gaatctttaa gtttcacttt tttgctcttt aaaaaagata atttagtttc tgcagttatg
     8701 attgcaacaa atatcaagat actaccaata accaattttg ctgtaataag ctcctttaaa
     8761 attattatag aaaatattgt accaaataca gcctctgtgg ataatattat agctgttcta
     8821 gttccatcaa ctcttttttg acatatagtc tgaaataaaa atccaattgt agtactaaat
     8881 acagctagat acatagttcc catgtaacca gataattcag cttccatctt taattgacct
     8941 gcaaatgttt gaactacaac agacattaaa aacgctactg taaattgaat agctgtcaat
     9001 gccattgggt tgttatcttt tgcaaactca cttgtaaaga atatatgaaa tgcaaatccg
     9061 aatgaacata taagtgttag aaaatctcca aaattaatag agaaatcagc ttctaaagaa
     9121 agtatcccaa ttcctataag agctaccaaa ctacttatta ttcctatttt atcaagtttt
     9181 cttctataaa gtataaatcc tatgaatgga actattacaa cattagctgc tgttataaac
     9241 gcatttttag atggtgtagt gtacataagt ccaatagttt gtactgtgaa tgctagaaat
     9301 aaaaatattc ctaaaatccc tcctgctttt aataattttt tacccatatt agcacgaatc
     9361 tgtttaaaaa acagtaaatt cataattata gctgccaaga aaaatcttaa agttatgatt
     9421 tgtagaggtg taagcccacc gtctaatgca aattgggttg ctacaaagcc acttccccat
     9481 ataattgcta taaatattag tcctatttct ccaaagtatt ttttcaataa agccccctcc
     9541 tttttatttt tttgcaaaat tataaacccc ctaaacagca agagagtttt tctataagca
     9601 ttactataaa ttaatatagt aaattttttc agtattttaa atctaaattt acatacattt
     9661 tgagctcaaa tttatatcca ctatttaaca gtaatatatt aatcattttt tgtaaacacc
     9721 tttttaataa atttttatat tttttatatt tttttaccta tactaaattt ttttatagat
     9781 atttttaata tatatgatac tttaatatta tgcaaataat gtcatataag ttacacttta
     9841 gttatcttag ttacaacttt gtcggtttac tttttctact ttcttcgata taattataat
     9901 atatttttga tacaattcga caaaaggggg aatgctgtaa ttatggtaaa aaaaagaaaa
     9961 aagaaaactg ttacacttac tatacttgtt gtaatagtta ttggtatttt ttctatggca
    10021 tgggcggaca tgtctagaaa aaaagaaatg aaagaaactc aagaaagact taaagctgaa
    10081 agattagaaa aagaaagaat tgaaaaagaa aaaaatgaac ctattattgg tgcaaaaaaa
    10141 gaagctgtaa aagaatatgg atatgacgca aaaatagttt gggacaaact taacaaatac
    10201 gactattcaa acaatggaga aaagatagtc tttcttacat ttgatgatgg accatctact
    10261 actaatactc cacaagttct agatatcttg aaaagacatg gtgttagagg aactttcttt
    10321 attaagggtg attctcttga aagaaaagga gctaatgaaa ttttaaaaag aacatttgac
    10381 gaaggaaatg caatagccca tcactcttat actcatgact ataaaaaact atatcctaac
    10441 agaagcctaa atctcgatac attcgtaaat gaacttaaca aaactgatga agctatgaaa
    10501 aaagtcttag gaaagaattt ttcctcaaat gtagttagat gcccaggcgg gtatatgtca
    10561 tggaaaaata tggaacctct aggaaactac ttaaaagaga aaaacatggc atcaattgat
    10621 tggaacgctt taaatgctga tgcagaaggt aaaaagaaaa atgcacaaga attatttgaa
    10681 catgcaaaga aatcttctga aggcaaagaa atggttgttc ttttaatgca tgatacttat
    10741 ggaaaacaag aaacagtaaa tgcattggac caaattataa catattttaa agacaatggt
    10801 tatcaattta aaattttaat ataataaaaa agcaagttta ttgaatgtac attttatact
    10861 caatagactt gcttttactt tactctattt tactagctcg atttttacaa attttttctt
    10921 tcctatttga acaactaatt cactctcttt tacaatagtg cttaaatcct ctactttttc
    10981 accatttacc tttactccac cttgagaagc taatcttcta acttcactct tactcttaac
    11041 aatctcatta gaaactagta cttcgattaa gtcaaatcca tcttccttaa cttgaattgt
    11101 ttgtatatct tcaggtattt gacctttttg gaatacagat ttaaatcttt cttcagcttc
    11161 tttagcagat tcttctccat ggtataatgt tactatttct cttgctaagt tcatctttat
    11221 atctcttgga tttactgaac cctctttcaa ctgtgattct attttattaa cttcatcagg
    11281 atgaacatct gtcactaaat tgtagtattt aattataagt tcatcaggaa tttccataga
    11341 tttttggtac attattcccg cttcttcatc tattccaata tagttaccta aactcttact
    11401 cattttttct ttaccatcta atccttctat taaaggcatc attattacta tttgagactc
    11461 cataccaaat tctctttgta atgatcttcc cattagtaaa ttgaatcttt ggtcagttcc
    11521 accaagttct atatcagctt ctaatgctat agagtcatat gcttgcatta atgggtagaa
    11581 gaactcatgt actgatattg gcatttgtcc ttcatatctc tttttaaaat cttctctttc
    11641 taacattctc gcaacagtaa tagttgctgc taattttatt acatcttcaa aatttaactt
    11701 agctaaccac tcgctattaa atcttacaat agttttttct ttatctaaaa ctttgaaaat
    11761 ttgttcttca taagtttttg catttgcaag aacttgctct gttgttaaag cttttctagc
    11821 tttagatttt ccagttgggt ctccaatttt tcctgtaaaa tctccaatta ttattactat
    11881 ttgatgacct aaatcttgta attgtttcat ctttctaaga actactgtat gccctaaatg
    11941 tatatctgga gcacttgggt ctagacccaa ttttactatc ataggctttc cttctttttc
    12001 tgatttaata agcttttccc ttaactcttt ctcatctatt aaatcatcta cacctttcat
    12061 aattattcgc atttgctcgt ctatcgattt catttttcat tcctcctaaa atatttatat
    12121 aatctataat ttttattttt ataataaaaa aagctttcat cctcttaaaa ggacgaaagc
    12181 attgcttacg tgttaccacc ttagttcatc aatatctcac gatattaatc tcttagagta
    12241 caattacata tactcgtgta ctataacgtg tactaatccg atatctctta atggttgtta
    12301 tctttcagag atagtgctct gagatgtatt caaactaaat caatttattc catttcacca
    12361 aactggaact ctctgttaaa taaaatctag ccctacttct tctcttcata gcatttatat
    12421 attcagatta tttcatcaat tacaagaaat ttttcttaac ttactcataa tatattatta
    12481 tttttatatt ttgtcaacct tttttattat tatatacatg tttttttaca ttatactact
    12541 tttttatatt ttttaccaat acccaaaatt tatttttcaa tcaaaagatt tatttgttta
    12601 ttgactatat tactctgact caatcttata tttattttct tatttaaaaa tgcgtttata
    12661 attattaaac caataactgt tacatcaaat ataaaaaaca gcaataaaat tattatcaaa
    12721 aataaaattc tagcatataa taattttcta aacttagcta ttataaagtt ttgtagtgac
    12781 atacatgagt ctctaatgat aaaatagaga cttatatagg ttattgatat aaagattgtt
    12841 tttaaataaa cagaatcaac tttaacattc aacagtttta gaacttcaaa aatggctaga
    12901 taagaaacac atatgtaaaa agcttttttt gagcaacata aaaattggtt taaacttcta
    12961 aaattcttaa atattaaaaa tttttttaga gaattagaat ctaaaatatg caatttattc
    13021 cctaaaatta aacttaataa atatatgcaa aacaccatac caaaaggaaa caatgctatc
    13081 attaaataat aaataaaatt tgtataccca ttaaaataaa tccatttgct atatccttga
    13141 aattctttca taaaactata acctattaat gttgaagcat atatatcaat aaaaaccatt
    13201 acaataagcc caaatactga accatatact aaatcatcca taaccattgt aggcttattt
    13261 attagatagc cacacattat accaagtaat acactctgac ttgcccaaat agcagttcct
    13321 agatttccaa gtaccagaga aattattaaa agcgaagtaa ttccagataa aatggtatac
    13381 tttaaatcac atttcaagca gattatacaa aaaaatactg gcactataaa atctaagtaa
    13441 atagcatatc caaatccagt actcacagct ataattgtta ctactatgaa taaagatgac
    13501 aataaagctg cttcagttaa ttttttagta gtattcaaaa cactgcctcc ataaatcata
    13561 aattttctaa ctaaagtttt gtttttaata tagtatatca ctttttaaaa atatttttaa
    13621 taaatataaa ttttaataaa ggtatttatg ttaaaaatgg tataatgtat cttaatattc
    13681 agttttttaa gatgctagga ggtaattact atgtttaaaa ttaaacactt taatgatttg
    13741 agtttagatg aattttatga aatagcaaaa tcaagatatg aggtatttgc ttgtgaacag
    13801 aagatatttt cattaaacga ttatgatgat atagataaaa actcttatca catattttta
    13861 aaagaaaatg gattgatttg tgcttacgct agaataattc ctaaagaata cagctcttat
    13921 aatgatgttt ccattggtag agttttagtt ctttctagtc atagaagaaa aggattagca
    13981 aaacaaatga tggattgtgc tatagatttt ataaaagtta acttacacga aaataatata
    14041 accttatcag cacaaactta tataaagaac ctttacttat cttgtggttt taaagaaatc
    14101 tcagaagtat acgacgaagc aggtattgaa catattaaaa tgagattata gtgttgacaa
    14161 gctaatattg ttaatatata atttaagtat aaattaaata ttgaattgat atttaggtat
    14221 ttgaaaatac cttacataga gtcagaaggc ttttgctttc tgactttttt attatcaaaa
    14281 ttcaataatt atatggattc aaagtgttaa tttttattct atattctact ttccccaaaa
    14341 gtaagaatta tacccaactt tgtatgatag gcatcttgcc tatctttttt ttgtaaaagt
    14401 aacaaggtaa taatataatg catagtttaa aataagaaga ttagcccagg ctaaatttca
    14461 agcatggagg tgctattatg aaaaacctta acgatattca agtgaaaaaa actgtcaaag
    14521 ttgaagatat attatctagt ggtaatttaa gagaaagaat gttagcgctt ggtcttacta
    14581 gaggagctgt tatagatgta gttagaaaag gtccaaaaaa taatttaact gtctataaaa
    14641 tcagaggtag caagatagct ctacgacaag aagaaagtag cttaatatta gtttctgaca
    14701 tttaaaaatt tctagcagga ggatattaat atggggttga cacataactc aactaaaatg
    14761 agttctttaa aagacatgtt tgatatagac aataaggaag accagtttgt aattgcattg
    14821 gctggcaatc caaatacagg taaaagtact gtatttaatc atcttacagg acttagacag
    14881 catactggaa actggccagg aaaaactgta gctacagctc gtggtaattt taaatataaa
    14941 aataccgaat atgcattaat agatttacca ggaacatatt ctttatttgc cttatcacaa
    15001 gaagaaattg tagctcgtga ttttatatgt tttggtaatc cagatgcagt aatagtagta
    15061 tgtgatgcta catgtcttga aagaaattta aatttagtat ttcaagtgat ggaacttaca
    15121 gataaagtta ttttgtgtat taatttaata gatgaagcta gaaaaaaagg cattactatt
    15181 gataaaaaac ttttagagga ttctcttggt attccagtta ttttaactgc tgcaagaaat
    15241 ggctctggta tggatgaact attggatact ttaaatgatg tatcttttga caaatataaa
    15301 ttaaataata aacctgttag atacaatgaa aatatagaga atgtggtaaa atcaattcaa
    15361 ccagaattgg ataatattat accaggtata aattctagat ggttgggact tagacttatt
    15421 gatggcgatg aaagtatatt tgaatctatg agtaattata tagacaaaaa ttctatagat
    15481 gctattaatg aagtaaagaa aaagattcca gacaatataa ataaacagaa aataagagat
    15541 gagtttacta aaatcaacta tgattatgct aaaaaactga gtgatgagtg ttgttctaat
    15601 gttgctaaaa aatcaactga tagagaagaa aaagtagata aaatattaac atctaaaata
    15661 tttgggcttc caataatgct cttattatta ggtactatat tgtggattac tatagaagga
    15721 gctaactatc catccacttt attatctaat ttattactcg gatttgagcc aagtatttcg
    15781 ggtatattaa atagtattaa ttgtccatca tggttaaatg atatgttggt tttaggatta
    15841 tacagaacat tagcatgggt aatctctgta atgctacctc ctatggcaat attttttcca
    15901 ttatttaccc tacttgagga ttttggatat ttgccaagag ttgcatttaa cttagaccac
    15961 ctatttaaaa aagcttgtgc acatggaaaa cagtgtttga ctatgtgtat gggatttgga
    16021 tgtaatgcag ctggagttat tggttgtagg ataattgact ctccaaggga aagacttata
    16081 gctatactta caaacaactt tgttccttgt aatggtcgtt ttcctacttt gatagctata
    16141 tctacaatat tttttagctc agttataacc aactctttcg tatcaagcgt agcaactgca
    16201 ctttgcataa ctttactgat aattctaggt gttataataa cattgttggt ttcttacact
    16261 ctttctaaga cgctattaaa aggagttccg tctacattta cattagaact tcctccttat
    16321 agagttcctc aaataggaag aactttatat acctctataa tagatagaac aatatttgtt
    16381 ttaggaaggg ctgtaatggt agccatacct gctggtgtca taacttggat atttgcaaat
    16441 atatatatag gagatttaag tatattatca catgttgcaa actttttaga ccctttagct
    16501 aaattaattg gtcttgatgg ttttatatta cttgctttta ttcttggatt cccagcaaat
    16561 gagatagttg tacctatact tttaatggct tacctagcaa ctggttctat gatagaatta
    16621 gatagcttta gtgctttagg tcaagttctt agagaacatg gatggactta tttgacagct
    16681 ttaaatgtta tgctgttcag tttactgcat tggccttgtg caacaacact tttaacaata
    16741 aaaaaagaaa ctggtagttt aaaatggact gccttaggat tcttaatgcc tactatactt
    16801 gcttttgttg tttgtttttt aacaactaca gtttacaatt tatttatata attagtatta
    16861 actgacagta gacatatatt agagaaacca tttaaaaatt tatatacttt attataaagc
    16921 atagagatat attcaatgat aagtaaaatt acttaacatt tgaatatatc tctattttaa
    16981 ttcttatctg gatacttgga ttgaaatttc agtcatataa ttatcttcat tactttcaca
    17041 aaaaaagtca agtatactct cctcatacga atttccaaca attttatatt cattttcata
    17101 tatatatttt ttcaattttt cgtaagattt atatgaatcc tcatattttc caatatgttt
    17161 tatgcaagca tacattcctt ctggctttac atatagcttt ttatccttta tttttttatc
    17221 tatcttcaaa taaaaacttg atatacttaa atagtcccca ttttttatat tttcattact
    17281 tactattact cccaactcat atccttgata caaatttttc gatttacaat aattggtaaa
    17341 atctattaat tccatcataa aatcactatt actcatatta ctcaaatcgc ttgatagaac
    17401 taaatattct tcaaactttt tggatatata aatttcttca ctattcattt tagaaattcc
    17461 ttcaactgtt atatttattc tattttgcat cttagaactt atctttctta gtttttttat
    17521 tttttctctt atcttttttt cttcttcttt aagcattttc acacaaagct ggggagttct
    17581 attttgtata tactctttta tttcctctaa actcattcct atttctctta atgattgtaa
    17641 tatatcaagt gtatcatatt gataaactga gtaatatcta taattatttt tatctttata
    17701 ttcaggagaa aaaataccta ttttgtcata gaaaattaat gtctgcttag atataccaca
    17761 caatttagca aattcacctg ttgtaaaata ctgtttttcc aagggttata cactcctttt
    17821 ctttttgttg tctatatagt tactatattc tttattattt actaaatcaa gtagttatta
    17881 ttttagaaaa tcaatatggt tttgatttta aagcttaaat atcagttaga tgtcagttat
    17941 aaatatttta ccctttccca tcataaaaaa gatatcccct cttaactttt ggatggaata
    18001 ttatatttat acttgttaga gtgaaaattg taataggtgc tacaataaga tatttaatta
    18061 aacataaagt taattattgt aataaaaaat ctttagataa attagagtta aagtacggaa
    18121 atatagatag agaaaaagct gttaaactag aaatatttta tcagtattta atagaacttg
    18181 aatatataga aatgagttta ttaataagaa gattagatac tgcaataaca tcattagcat
    18241 tagtatctat tataacaata gcatcatatt atttaatcag aaaaaatgtg taattatgta
    18301 agctatttat aataaaaca
//
LOCUS       DAYAOX010000065        18293 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.065, whole genome shotgun sequence.
ACCESSION   DAYAOX010000065 DAYAOX010000000
VERSION     DAYAOX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.065"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            163..2262
                     /gene="nrdE"
                     /locus_tag="ACGI57_001738"
     CDS             163..2262
                     /gene="nrdE"
                     /locus_tag="ACGI57_001738"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFG9716818.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDKWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2275..3246
                     /gene="nrdF"
                     /locus_tag="ACGI57_001739"
     CDS             2275..3246
                     /gene="nrdF"
                     /locus_tag="ACGI57_001739"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFG9716819.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3322..3948
                     /locus_tag="ACGI57_001740"
     CDS             3322..3948
                     /locus_tag="ACGI57_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9716820.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            4343..4972
                     /locus_tag="ACGI57_001741"
     CDS             4343..4972
                     /locus_tag="ACGI57_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716821.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            4973..5755
                     /locus_tag="ACGI57_001742"
     CDS             4973..5755
                     /locus_tag="ACGI57_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9716822.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            5748..6626
                     /locus_tag="ACGI57_001743"
     CDS             5748..6626
                     /locus_tag="ACGI57_001743"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716823.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            6659..7618
                     /locus_tag="ACGI57_001744"
     CDS             6659..7618
                     /locus_tag="ACGI57_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9716824.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            7933..8625
                     /locus_tag="ACGI57_001745"
     CDS             7933..8625
                     /locus_tag="ACGI57_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9716825.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            8866..9639
                     /locus_tag="ACGI57_001746"
     CDS             8866..9639
                     /locus_tag="ACGI57_001746"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9716826.1"
                     /translation="MYLGSSFLGRNISVILKQQHKLYQSMIQEHKKIHSEHLTFINQW
                     IHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLDMAMHFARADNFQKDFIVEK
                     AKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDVKWMKFIIGQLITNGVKYSK
                     DYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDPFFTGENGRKFGESTGMGLY
                     LVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV"
     gene            9907..10758
                     /locus_tag="ACGI57_001747"
     CDS             9907..10758
                     /locus_tag="ACGI57_001747"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFG9716827.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            10862..11608
                     /locus_tag="ACGI57_001748"
     CDS             10862..11608
                     /locus_tag="ACGI57_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716828.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALLRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            11608..13524
                     /locus_tag="ACGI57_001749"
     CDS             11608..13524
                     /locus_tag="ACGI57_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9716829.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVIMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKSISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFFIVR
                     NNYLKEIRLNLVNF"
     gene            13804..14037
                     /locus_tag="ACGI57_001750"
     CDS             13804..14037
                     /locus_tag="ACGI57_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFG9716830.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFISTKW"
     gene            14233..14661
                     /locus_tag="ACGI57_001751"
     CDS             14233..14661
                     /locus_tag="ACGI57_001751"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFG9716831.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKNFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(15115..15885)
                     /locus_tag="ACGI57_001752"
     CDS             complement(15115..15885)
                     /locus_tag="ACGI57_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716832.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLSL
                     NKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTFYCCGNCDSEISLSQREHFKI"
     gene            16209..16754
                     /locus_tag="ACGI57_001753"
     CDS             16209..16754
                     /locus_tag="ACGI57_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFG9716833.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            16786..17922
                     /locus_tag="ACGI57_001754"
     CDS             16786..17922
                     /locus_tag="ACGI57_001754"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HFG9716834.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            17926..>18293
                     /locus_tag="ACGI57_001755"
     CDS             17926..>18293
                     /locus_tag="ACGI57_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFG9716835.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKV"
BASE COUNT         7204 a         1826 c         3133 g         6130 t
ORIGIN      
        1 taaagcactg actatatgta gtgcttttgt ttttgaaaaa tacttattgc atttctataa
       61 ttatagatta attttgggat ttcaactgat aagtaccaaa agaaataata agcaaattaa
      121 gtgaaattat ggcacaagat tatgttaaaa ggacggtaaa taatgaattg gatagagctt
      181 aacaatcaag tgataattaa aaatgaacaa ggcaagtacc aattggaaaa ggataaggaa
      241 gccttaagta gttacataga agaatttata cagccaaagt tgagaaaatt taataaccta
      301 gaagaaagat tgaagtattt aatagatgaa ggttattatt caaaagaagt aataaatcag
      361 tatagcatag atgttattaa aaatatatat gatatgattg ataaacataa gtttgaattt
      421 caatcatata tgagtgcaaa taagttttat caaaattatg cattaaagag caatgatgga
      481 aaggaaatat tagaaacata taatgataaa gtgttaattg tagctttgac actaggaaat
      541 ggcgataaag atttagcttt aaacttggca gataaaatag taaaacaaga atttcaacca
      601 gctacaccta cttttttaaa tgctggaaga aaaagggctg gagaaatggt atcatgtttt
      661 ttactatctg tagaagatag tacagaaggt atatcgtatg ctatatcttc atcaaatcac
      721 ttatcaaaga ttggtggagg agttgcacta aatttatcta aacttagagc atctggcgaa
      781 tctatcaaag atatagaagg tgcagctgga ggtgttgttg gagtagctaa aatgttagaa
      841 caatcattta gttactttaa tcaaatggga gctagacaag gttctggtgc ggtgtacctt
      901 actgtattcc atcctgattt tgaattgttg atggatacta aaaagataaa tgcagatgaa
      961 aaaataaggc ttgctacatt atcattagga gctataatac cagataaatt tatggagttg
     1021 gcagaaaaaa atgaagtagc atatgcattt tatcctcata ctgtgtataa gaaatatgga
     1081 gtttctcttg atgagataga aatggataag tggtatgata aactggttaa tgatagtgat
     1141 ataagaaaaa aagaaataaa tcctaggcag atgttgacaa aaatagcaca gatgcaacaa
     1201 gaaagtggat atccatatgt agtgtacata gatacagcta atagagaaca tacattaaaa
     1261 gatgttggaa ttattaaaat gtctaactta tgttgtgaga tatttcaata tcaaacacct
     1321 tcagaaatag aggggtatgg aggaaaaaat gaatggggac aagatataag ttgtaaccta
     1381 ggttctttaa acatagccaa tgtaatggat aataaaacta tagaaagtac agttgaaaca
     1441 gcaataagag cactgtcatt tgtagcagat agcacagata taaaaccagt gccaacggta
     1501 agcaatagta acagcaagtc tcattctata ggattaggag ctatgaattt acatggatac
     1561 ttagtaagag aaaatatttt atatacttca gatgatgcta tagatttttc taatgtattt
     1621 tttgcaatgg ttagatacta ttcaattaaa gcttctatga aaatagctat agaaagaaat
     1681 caaacttttg aaggatttga taaatcagaa tatgttaagg gaagaaatag caaggtttta
     1741 tcaaaatact atgaacaatc ttatctacct aagtcagaaa aggtaagagc attatttgaa
     1801 gggatttata taccaacaaa agaagactgg acaaaattat tggatgaggt aaaagaaaaa
     1861 gggatatata atgcatattt aatggcagta gcaccaactc aaagcattag ttatgttcaa
     1921 aatgctacat caagtataat gccaattact gagccagtag aagtaagaac atatggagac
     1981 tctactacaa tatatccaat gccattttta acaaatgata atatgttgta ttatcaatca
     2041 gcatacagga tggatatgag aaaggtcata gatttagtgg caacagttca aaaccatgtt
     2101 gaccaaggta tatcaacaac actgtttgtt acagatgaaa agacaactag agatatagct
     2161 agacattaca tatatgccta taaaaaaggt ttgaaaagtt tatattacac aagaacaaag
     2221 atgacaagag atactcatga gtgtctagtg tgttcagtat agggagagaa atatatgaca
     2281 tttaatttac ataaaataca taatgcagtt aactggaata gagaagaaga tggatttaca
     2341 caagcttttt gggaacaaaa tgtaaaacaa ttttggcttc ctgaagaaat atctgtttct
     2401 aaagacataa aggtatggaa tgaactaagt aataaagaaa aggaattata taaaaaagta
     2461 ttgggtggtt taacattact ggatacaaaa caggggaata atggaatacc atctatgatg
     2521 agtttaacag aaaacctaca aagaaaagct gtcttgtctt ttatggggac tatggaagaa
     2581 atacatgcaa aaagttattc ttctatattt atgactctac tttctaattt agaaatagat
     2641 gaattatttg aatggataga aacagaacca acattacaaa gaaaggctga tttagtttta
     2701 gctcaatacg aaaatactac aaatcaagag ggattatacc tatcaatggt tacaagtgta
     2761 tttttagaga gtttcttgtt ttattcagga ttcttttatc cattgtatct atctggtcaa
     2821 ggaaagatgg tagctagtgg agaaataata tcgcttattc ttagagatga gtctttacat
     2881 ggtaagtata taggattgct agcacaagaa atatacgatt catttgataa aatggataag
     2941 gagatgttag aagagaagat gtattctata ctttatagcc ttatggagaa tgagatagag
     3001 tatacaaatg ttatatacag agaaagtggg cttgaaaaag aggttgtgaa ctttttaaaa
     3061 tacaatgcga atagagcttt agaaaatcta gggtttgaga agctatatac tgtagatgct
     3121 ataaatccaa ttgtattaaa tggtcttagc actgaaacca aaacacatga ttttttttca
     3181 actaagggta atggatacca aaaaggagtg tatgaggaat tagaggatga agattttata
     3241 atataattta taattatgac acactgttgt cacttttagc gtgataatat caatgtataa
     3301 aatatatatg gaggtatttt tatgaaatat gaatggagaa aaagagataa agagttatat
     3361 ttacctaaaa agaatcctgt aatagttgaa ataccagaac agaaatttat catgttaaaa
     3421 gggagtggag acccaaattc taaagaattt actgaagcaa taggagtact ttattctttg
     3481 gcatatgcaa taaaaaccat gcctaaaaaa ggaattgtac cagaagggta ttttgactat
     3541 acggtttttc ctttagaagg tatatgggat aagggagaaa aaagtaaagg cttagatgta
     3601 cttattaaag aagatttaat ttacactata atgatacgtc agccagattt tgttacacaa
     3661 gacctgttgg ataaagctac tgatatagta aagaagaaaa aacctcataa tcttcttgaa
     3721 aaagttagat ttgggtctat taatgaaggg ctatgtgttc aaatgcttca tataggttca
     3781 tatgataatg aaccagaaag ctttgacatt atgaacgaat tttgcaaaaa aaataaactt
     3841 ataagaaaat cttacacaca tcgtgaaatt tatataagtg atgctagaaa agtatcgcct
     3901 gaatcattga agacggtttt acgttttgaa gtagaaaggg aagtataaat attttgaata
     3961 atttataaga gttataaaaa gtgaagtctg ttgagacttc attttttgat gcaattttaa
     4021 aattaaattt gatgtttcat aatattttaa aaactttcta cttttattag tgtgaataac
     4081 tgttgctatt aaataataat actaattata taagtattat tatataatat ttatagatta
     4141 ttatataaat agattattag taagatgtat gcattattaa ttaaaataaa ttacatatac
     4201 tatttaatat ttcatttaaa atattaaaaa atgtagataa aaattgtttt attaagaaaa
     4261 aattaatata ttgacaaaat ttaagtgctc atttaaacta ttaattgata ataatattca
     4321 atattaaata ataggaggcg ttatgaataa atatcttcta ataaatccag tggcagaaag
     4381 aatgtatgga gaaaagtttc accttattaa agcaaatttg ataaaaaagg ggtacataat
     4441 agcagaatgt gaaccacagt tagaatatgt aaaaaagcag tacaaagagt atacaaaaaa
     4501 gactgaaaat acaatgttgg attgtagatg tccagaaagt ataagtcttt taaaaagaaa
     4561 taatttgata aatgattttg aagtacctat gatagaacca atattagtaa ggacttgtag
     4621 ggttttatac gataaatata taaaaagtaa agatgatatg ttggtagtaa cctgtccatg
     4681 tacacaatta agagactttg caagtgaaaa gtttaaggat aagggtaatg ctatgtttta
     4741 tacatggaaa gagtttgctg aacaagaagg tgtaaaatca cttggaaaaa tagatgaatc
     4801 accaattcca ttaggatatt tcaaagatac ttttgaaaaa gttcttgaag tatcaagtga
     4861 agacgaaata ttaagtgaaa tacaaaaaat aagaaatggc tttgaagata agtatgacat
     4921 agtagaaatg ttttattgta aagatggttg taataatgga gatggtttgt aaatggtcaa
     4981 taaatttaat aataagtttt taaggctagt aaagagtgtt ttgatatttt ttataattct
     5041 tttattatgg aaaattacaa attatcttgg tatttggagt gattatatat taccatcacc
     5101 tgaaaaggta tatagtacat ttttaaatat gataagtgat ggttcaatat ttattaatgt
     5161 atatgcaagt atgaaaagag ttttgatagg ttttgcaata agtactgcta taggtgtgcc
     5221 tttagggata ttctttggaa tttatagtgg agtatacgaa tactttaaat ctctaataaa
     5281 ttttttaagg aatacacctc cccttgcttt aataccaatg cttatattat ggtttggtat
     5341 aggagaggaa tcaaaaatta taattatagt tttagcatcc tttttcccaa tctttacaag
     5401 cacattgaaa gggattaaaa attgtgactc aaaactgatt gaagtgggta gagtatttga
     5461 gttttcaaag cttcagataa tatttaaaat aataatacca aatgccattc ttgatattgc
     5521 tgttgggtta aagttagctc ttggatatag ctttagagcc attattgggg cagaacttgt
     5581 agcagcttca tctggtcttg gatacttgat atctgatgga aaagaaatgt ctagaacaga
     5641 tgttgttatt gtaggtataa tcgtaattgg acttttagga ataattacag attacatatt
     5701 ttcgattatt gtgaagaagg taagtaaggg aaagatggtg gaagcatatg aataatttag
     5761 tatgtgactc tggatatata attagaaatt tatataaaag ttataaagtt gacaatgaag
     5821 aacatgtagt tcttagtgat atctctttaa acatagatag aaaccatata acagtaattt
     5881 taggtgaaag tggttgtgga aagactacct tgcttagagc aattgcatca cttgaaggta
     5941 taaatagtgg tgaaattagg tttattaatg ataataaaga gtatagacct aatgttggtt
     6001 ttgtttttca ggagagcaga ttaatgcctt ggttaaacgt aagtgaaaat atagcttttc
     6061 ataatcaaag aaaaaatact attttaaaca aggtgcttag aaaatttaaa cacaataaaa
     6121 gaattagcag taaaattata aatgataatt ttagaagctt agaaacaaat gataatttga
     6181 acaaatcaaa taaatataat aggaaaagta atattgatgt agaaaaatat cttaagatga
     6241 tgaacttgca gaaatttaaa aactcttatc caaatgaatt gtcaggaggt atggcgcaga
     6301 gagtaagcat agctagagct ctttctttca atccagatat gttgcttatg gacgagcctt
     6361 tttcggcatt agattatttt acaaggattg atatgcaaaa tgaagttata agaatacata
     6421 agtcaactaa taaaggcgtg atatttgtaa ctcatgatat tgatgaagcg ttaaagatag
     6481 gcaagaaaat agttgttttc acagacgaaa gaaaagtaaa agaatttaat atagaagatg
     6541 gttataatag agatttaact tcagattact atattaaatt aaaaaaagat attttaatga
     6601 caatgaaatc aaataaaggc atttaaagaa attaataata ttttaaggag taaagtaaat
     6661 gaaattaaaa aagctattga taggtataac aatattaaca cttgcaacag catctttagc
     6721 tggatgtgct aaaaaaaata atggagaaaa actttcagaa ataaatttaa cttatgtaaa
     6781 atcaccatta aatgttcctt ctataattca aaaacaggat gatttatttg gaaaggaatt
     6841 taaaaaggat aatataaaag ttaatttcca cgaaattact acaggtccag agcaaacaca
     6901 agctttagct gctggagaaa tagacttctt acatgcacta ggtggtacat cagctttaat
     6961 agctgcatct aatggagtag agttaaagat attaaacact tatagcaggt caccaaaagg
     7021 atttatgatt ttgactaata ataattcaat aaaatcagca gcagatttag taggtaagaa
     7081 ggtggcaggc ccaaaaggga ctatattaca tcaggtattg atttcagcac ttgataaaga
     7141 aggattaagt atggatgatg tggaatttgt taatatgggt attccagagg catcagcagc
     7201 tctttctgat ggcagcgtag atgctgcatt gattgctggt ccagcagctc ttaaagctat
     7261 gaagtcaggt agtaaacttg tagcaaatgg tgaaggttta gtagatggta taatagtaac
     7321 tgctgttagc acagactttg cagagaaaca tccagaaata gttgaaagat ttatgaaggt
     7381 tgaaaaggaa actttagaat atgtaaataa taattttgat gaagctatgg aaaaagtagc
     7441 taaagaagtt gatttaagtc ttgaagaaac taaagaatta tatgcttggt atgattttag
     7501 tttagatata acagataaag acatatcttc gcttgaagat acacaagact tcttaataaa
     7561 aaataagtta caagagaaaa aagtgaacat aaaagaatta atctataatg ctaagtagaa
     7621 actatgataa aataatacta agtaagaaat atagtaaaag tttaagttac cttattaagg
     7681 taactttttc tattttgaat tagtaatttc aatttatttt agaatgatta gttgataata
     7741 atgattaatt aatcataaaa agatactttt ttatagagtt ttattaagat gtctaataat
     7801 atggttatac ttatgaagtc agtaattgtg aagttagtaa tatgattaaa tttatatatt
     7861 gtaaaaagat tataatatat taaaattgga ctgttaatat gatgagtaaa taggtatatt
     7921 aggaggaatt atatgagtta taaagtatat attgtggaag atgatatatc aataagtaca
     7981 ttacttaaag attatattga gaaatatgga tttgaagtga ttgttgaaga aaactttgat
     8041 gatataatga ttacatttga aagttttaag ccacacttag ttctattaga tgtaaattta
     8101 cctaaatttg atggtttcta ctggtgtaga tgtattagac aacagtcgaa tttacctata
     8161 atttttatat cagctagaga tggtaatgta gaccaaatta gagctttaga aagtggagca
     8221 gatgattata taacaaaacc tttttactat gatgtagtaa tggcaaaaat aaagagtcat
     8281 ataagaaggg tatatggtga atattcacct aaaatagacg aaagaatagt ggagctagat
     8341 ggattaaagt tttatccaga gaggttagaa ctagaattaa atggtgagaa tctaattata
     8401 actaaaaaag aaggtatact gattgaatgt cttatcaaga gatacccaaa agtagtaagt
     8461 agagattatc ttttagaaaa gatatgggat gatattgagt ttgtagagga aaatacttta
     8521 aatgtaaatg ttagcaggat aaggaaaaga tttgaggaat taggtataga taatgctgtt
     8581 cagactgtcc gagggttagg atataaatta aataagaatt ggtaaggaga aagtatgaag
     8641 ttatttttaa aagattataa aggttatatt tttatatatt atataggcat agttatgaca
     8701 attatttact gtaatcttat gaagtttatt agttttggag agagtttata tattctttta
     8761 tttagttctt ttatattgtt atgtttttta atatataagt attataaaaa tagagaagta
     8821 tataatctgt ttgaaaaagg agtacattct ttggaggagt catttttgta tttaggaagc
     8881 tcattcttgg ggagaaatat atcagttatt ttaaaacaac aacataaatt gtatcaatca
     8941 atgatacaag agcataaaaa gatacatagt gaacatttaa ctttcatcaa ccaatggatt
     9001 catcagatga aaacaccaat atcagttata agccttcagc ttcaagatta tgagggagaa
     9061 gaagtaactg aaaatataag aaaagaaata gatagattaa ataaagggtt agatatggct
     9121 atgcattttg caagggcaga taattttcag aaagacttta tagttgaaaa agccaaatta
     9181 aatgaaattg tatctagtat tgtaaatgaa gaaaaaaggt tatttataaa aagtggaatg
     9241 attcctaaga ttcaagtaga caaatttact tatgtttata cagatgtaaa atggatgaaa
     9301 tttataatag gtcaattaat tacaaatgga gtgaaatatt ctaaagatta tagtaactac
     9361 ctaactataa aatctgaaga aaataataaa tatgtaattt taagtataat agatgaagga
     9421 attggaattg tttctaaaga tttaaaaagg gtatttgacc cattttttac aggagaaaat
     9481 ggtcgtaagt ttggagaatc tacaggtatg ggtctttatc tagtaaaaaa ggtatgcgat
     9541 gatttaggtc ataaagtaag tataaaatca gaagtaggaa aaggtacaga agtttcaatc
     9601 tactttaata aagatgtaaa tagtttaata aaggtgtaaa tagtttgata aagatgtaaa
     9661 ttatctgtta caatagggtt tttagttaat tgtacttttt tattatacaa tttattaaat
     9721 gaaatattca tagttaaata atcaaaattt aagagcgttt atttgcaaac cttacataga
     9781 tgtaaggttt gtgtaatgtt tgcagatagt taaattttct aatctaaatt acaattaata
     9841 taagaaataa cataagagaa ataacataaa aaaagaaatt ttgtaaaaaa ggaaggatgt
     9901 ttattgatga gtttagatgt tatttttata ttgaaatcag tcataatagc tatagtagag
     9961 gggcttacag aatttattcc agtttcatct acaggtcata tgattttggt tggtaattta
    10021 atagatttta aggggcaatt tgctgaaatg tttgaggttg taatacaact tggggctata
    10081 ttagcagtag tagtacttta ttggaaaaag ataaaagata gcgttataga attttttaaa
    10141 tttatattta ctggtggaaa agaaggaaag attggtttta gatttggaat gaatgtaatt
    10201 ataggttgta taccatttgc gattatagga gttctatttt acgataatat aaaatcattg
    10261 tttaatttac aatctgtaat tataggattt atagtgggtg ggattttatt attagtagta
    10321 gaaactttgt ttagaaaaaa gaatcattcc acagataata tagataaaat tactccaata
    10381 caagctttaa aggttgggac attacaagtt ttgtctgcat ggccaggtat gtcaaggagt
    10441 gcatctacta ttatgggtgg atggatagca ggacttaatt ctcctacagc agctgagttt
    10501 tctttctttt tagcagtacc agctatggtg gcttcttcag gaaaagattt atttgaattt
    10561 gattattcaa taatgacacc aacattgtgg attgctttgg tagttgggtt tatagtagct
    10621 tttattgtat caataatagt tatggaaaaa ttcgtgaatt ttttaaagaa aaaaccaatg
    10681 agagtgtttg ctgtatacag aataataatg ggtgtagtac ttgcagtatt agcttttact
    10741 aacataatta gtgtatagaa aaaatagatt tatcatctat aaaaattaaa ctaagataaa
    10801 ttataattaa caattatata tatgttattt caaaaattaa attaggaagg atagttaaat
    10861 aatgaaaatt ttaactgtaa ataatttaag taaagtatat ggtaaaaaaa taattttcaa
    10921 cgcattaaat gacataaatt ttagtataga agatggagaa tttgtaggaa ttatgggtcc
    10981 atctggtagt ggaaaaacta ctcttttaaa tatgatttct actattgaca agccaactac
    11041 aggaactatg gaattaaagg gcaagaatcc tttattgtta agaggtgagg aacttgcatt
    11101 acttagaaga agagagttag gttttgtatt tcaggatttt aatttattgg atacattaac
    11161 tataggagaa aatatagtat tgccattgac attagataag gtcagtgtaa aagagcaaga
    11221 tgagagattg aatgaagtat ctacaatatt aggtataaaa gatttattag gaaaaagaac
    11281 ttttgaagta tcaggtggtc aagctcagag gactgctatt gctagagcat tgattaacaa
    11341 tccatcaata cttttagcag atgaaccaac aggtaattta gattcaaagt catcaaaggt
    11401 tgtaatggag ctatttcaaa agataaataa agaaaataaa gttactacta tgatggtaac
    11461 tcatgaccca ttagcagcga gctactgtag tagaatctta ttcataaaag atgggtctat
    11521 atataatgaa atatataagg gtagtagtag agagcagttt tatcaagaga taatggatgt
    11581 acttacatta ttgggaggcg ataactaatg aatataaaac agtttgctat taacaatatt
    11641 tcaagaaata ttaaagcgta tttaggatac ttagcaagta ttgttatatc atcttcactg
    11701 ctattttctt ttattatgtt tacaagtcat ccagatttag atgtatctca atttcctgat
    11761 tatttaaaag aaggtctcaa gatgagtaag ataattgcat atctattctt attcttcttt
    11821 gtgttttatt cagttagtgt gtttcttaaa agtagatata aagaatttgg aatactttat
    11881 attacaggta tatcaaagcg ccaagtaatg aaattaatct ttatagaaaa tatgataata
    11941 aatatagtat cttctgtaat tggaataata ataggcttaa tctttgcaaa gatattttta
    12001 gtgattatgt ctactttttt agaattatca ccattaaaat tctatatacc atttaatgcg
    12061 atattgtcta ctttaatcta ttttatgatt ttagctgtat taacatcaat atttacttct
    12121 tttatagtta aagaaaatca ggtgttgaag ttacttaaag gtacaaaaac tccaaaacca
    12181 gagcctaaaa catcactaat gcttgctata ttaagtattt gtttgtttat aatagcgtat
    12241 tataatgcta ttacctcaac cgaccaatat acaacatatc tgcgcttagt tccagttaca
    12301 tcccttacta tagtagcaac ttacttgttc ttttctcaat ttagtgtatt ttttatgaag
    12361 aagctaaaac taaacaagag attttataga aaaaatacaa atatgctttg gatttcgaac
    12421 ttgatttata aggtaaagga taatgctaga atatttttct tgataactat aacttctgca
    12481 gtagcattta ctgctatagg tacagtttat tccttttgga aagatgtaga gcgtcaaatt
    12541 aatttaatat atcctaacac aatatattat tcaacaatga cattgcataa tgaccctaaa
    12601 aagcctgata gcaaagaaaa agacaaggaa agaatgatgt ttatagaaaa taatctaaaa
    12661 aaagagaatg tagactatac aaggactaat ggtgaattta aaactgtatt tcccaagaaa
    12721 agtgatttta atgtcagaat tatgaaagaa tctaagtact tagaaattac agaaactata
    12781 ggggttaagt ctataagttt tgaagataat gaatgtatat cattattatc atcaagttta
    12841 ccaggaaata ataacgtcaa agaaaatatt attgtaggga ataggggttt aaaggtggca
    12901 aaacaagtgg aagaatgtgt tatgccagca tattacaaaa atatgtatgt ggtaaaggat
    12961 aatttttatg atagtataga atcaaaattt atagtggata gatttactgc atttgaagta
    13021 gaggattcaa gtaaaactat agagatatgc agagaatttg aaaataaatt taataatgag
    13081 tcagggatgc aaccatattt gtttttctct aaagaattaa tgataggaac tgggaaatta
    13141 atgtattcta catttatatt tttagcaata tttataggtt taattttctt tgttactaca
    13201 agtagttttt tatataataa attttatatg gattgccaag ttgataagaa aaagtatgaa
    13261 cagttaaata aattgggtat gacatataat gagattaaaa aagcttcaac aattgagatt
    13321 ggaatagtat ttttgtttcc ttatgtagtg gctgttatac attcaatatt tgcacttact
    13381 gcacttaaaa attcttttga tatagaagta gcatcatcag ctgttttggt aatgggaagc
    13441 ttgttcttgg ttcaaatagt ttattttttt attgtaagaa ataattattt aaaagagata
    13501 aggttaaatt tagtcaattt ttagttacta ttattaaatg ataataaaaa gggtttaaaa
    13561 ttaattaata ttactggaat tatagatgtt tattttttca gtaatatttt ttattgtatt
    13621 aactattaaa attttgtaca aatataaaat ataatatggt aaatgtcaaa attataaaaa
    13681 aattctactt atataaaata taaagaggtt atatgtacat atgtgtagaa ataaataata
    13741 aaaatatcaa ttaatgttat tagggggggg ttactcatta gtaaattagc taatacactt
    13801 agaatgatga tattaattca aagcagaggc aaaatgaaat gcaaagaatt atcagaagag
    13861 ttggaagtaa gtgaaaggca aataaaaagt tacaagatgt atttagagca agctggtata
    13921 tttataaatt caacacctgg gatttatggt ggttatgaaa tagataaatg taatagcata
    13981 tcattaatta aattattaga tagtgaagtt tcaattttta tatcaactaa gtggtagata
    14041 agtagaatat tagcaatatc aacgagtatt tagaaactta ttttatatta ataaagcagt
    14101 atataaaatt gtagaatcaa caatagcata tactaaaaca tccaccttat attaactaaa
    14161 tcatatctaa ataaaataca cttacctaca aacattataa aatcaataca aaaatgaggt
    14221 gaaacaaaat ttatgataaa gaaattaaac agtaaagaca taaataaaat catggaaata
    14281 tgggaaaaaa gtacaatcaa agcacatgac tttataagta aagaatactg gcaaaataac
    14341 tataatactg ttaaaaacga atatatacct atatcagata catttgtata tgatgatgga
    14401 gatgaaataa aaggatttat aagcataata gataaaaact ttataggagc tttatttata
    14461 aagcccaaat accaaaatct aggtatcgga ggtaaacttt tagattatgc aactaaaaaa
    14521 tataaaagtc taagcttagc agtatataaa gataataaaa aagcagttgt gttttataat
    14581 aaaaaaggtt ttaatatagt aaaagaacaa gtaaatgaag attcaggatt taaagagtac
    14641 ataatggaat atagtaaata atatgattac attctattaa aaggataatt aatttatttt
    14701 ctagttgatt ttttaagagt aatttttaaa atagactata tgaagataaa gatatttatt
    14761 atagatgtaa gttaatcaga attgatatag ctacaaaaga ggaaaataaa attgtatggt
    14821 gtatttcaga tttaacaaat ttactattta aagataataa gtttgatata atattaaaga
    14881 tactttcacc tataaactat agagtattga aagaatttta aataattatg gaatgctaat
    14941 aaaggtttgt taaaatttaa taattattag attatagaaa attgccatga aattagttca
    15001 tcaagaacta gtttcatagc aattttaatt cttataagtg tatttatttt tacagattta
    15061 aaaaacttat acagtatcta ataatgtaat cttattactt tagcctataa atatttaaat
    15121 cttaaaatgt tctctttgag ataagcttat ttcagagtca caatttccac aacagtaaaa
    15181 tgttgagaca tctttaaatt tttcagtatt ctcatcaaag ttcatttttt tctttgcaat
    15241 aattcctacc ttagttaaat tagaaccaca attattacag ttttgaattt tgcataggtt
    15301 atatagttta tcaagtgtaa tatcttctaa taattcatca caattacaac atctgataga
    15361 agaaacttca ctacacattt caataggttc agcttcttta gaagaaaatt tatatgttat
    15421 gtcatttgat actttataac ctgtatcaaa tatattagaa ttacaacttg gacataagta
    15481 ctttggaaga tatatatctt ttttttcaaa agtatcacta ctaatattac agtttaattc
    15541 atctaaaatt ggtctttcat ctaggttatt acctttattt aagcttagaa gataattata
    15601 aattttttta tcttcattct gttttgatgt atctaacaaa tgctccatta tattagtgta
    15661 ttttttgttt tttgaaggca tgtttaaatc cagataattg ttttcaatag caaagcatac
    15721 tatattgaaa tagttatatt taactgcaaa aaataataac atttcatcaa atactttatc
    15781 tattattttg tcagaaatac ttatccaaaa atctggtgac tctaagtaaa ctttcctact
    15841 aagttcatct aactcttcat ctatattatt ttcattaact ttcatatcat cacttccttt
    15901 taaatatctt gtgtataaac tatttgttac tattttttat ataaaataaa atcttatata
    15961 aaaatatatc atattataat ttacaagtat agtctaaata gagctgtttt taagcaaagt
    16021 tttataaatg actattaaaa tataaagtaa cagtataaag tttacttata aattaaatgt
    16081 aaacctaaat aattataata gttgacaaaa aaacgtcttt attttatact aaaacaaagc
    16141 tttaatagaa gctttgtata tgtacatata aactaaatga aataagacat atgaagggaa
    16201 ttataaaaat gaaaaatact aaagtaaata ttcaagattt aactaaaatg tcaatatgtg
    16261 tagcattatt gtgtatatca tcttacatat atataccatt gccatttact ccagcaggag
    16321 taacagcaca aacaataatg ataaatttaa tagctttgat tttaacacca agacaagcat
    16381 ttagtacagt tggtgtatat ataatgatag gtttaattgg gattccagta tttggtggag
    16441 gtacatctgg tattggaaag ttattaagcc caacaggtgg tttttatttt gggtttttgt
    16501 ttgcagcact aattattagt ttattaaaag gacgaaataa tgatattaag agatatatat
    16561 taataacaat ttttgttgga atgcctatta tatattttat gggaacagta tttatgtgct
    16621 attttaatca aatgacagta gaagcagcac tttttgcagc agtagtacca tttttaattg
    16681 gagatacaat aaagtcagtt attgcatctt ttttaggcac taaactgaac aatgtactca
    16741 gaagaaatct atgataaatc tataatataa ggaagggtgt aatgtatgaa ggaagttttt
    16801 atattaggag gattgagaag tcatataggt ctaaaaaatg gaatttttca atttgttcaa
    16861 cctgaattac taggagcaag tgttttaaaa gatttaatta aaaagtatga aattgacaaa
    16921 attgatgaga ttatatgtgg aaatgcagtt gggacaggag ggaatatagc tagattaatg
    16981 actttaacag caggagtgtc aaatgaagta cctgctttta ctgtggatat gcagtgtgct
    17041 tctgctatga tgagtataga catagctttt tcaaaggtta aatctggtca gtgtgattta
    17101 attattgcag gtggttttga aagtagttct ctgcaaccaa tgcgcacata tcataagaat
    17161 gataagcgat ataatacaaa taatccaaat tatacagttg ctcaattttc tcctgatgat
    17221 aatagccaaa atagtatgct cgaaggtgcg gaaagagtag cagagttata tcaaattgag
    17281 aaagcagact tggatttttg ggtaaaggaa agtcataaaa gagcaaaaga agcaagagag
    17341 gaaaaaatac ttgaagatat tatatctcct ataaataata gtacctatga tgaaggtata
    17401 cgtgataaga tgagtcaaag gttattagat aggatgccat caattttagg aaaagaaact
    17461 attacaaatg cagcaaattc atgtcttatt aatgatggag cttcatttat tataatatgt
    17521 tctaagaagt atttagaaca tgtaaaaaag aagcctaaag caaaaattat taacacatgc
    17581 acgataggaa cagatgcaac cttaagtcca acatcagcga tacaagcaat ggataaactt
    17641 ttagagatag aaagccttaa ttatttagat gtatctgctg ttgaatttaa tgaagcattt
    17701 gcagttatag atgtgttgtt tcaaagaaag tatccagagt taatagatag atataatata
    17761 tttggtggag ctttagcgta tggacatcca tatggtgctt caggtgcaat tattgcatta
    17821 catcttttaa aagcattaga aaagactaag ggtagatatg gaatttgctc tatagctgca
    17881 gctggtggtc taggttcagc tttactgctt gaaagggtgt agattatgaa ctattatgaa
    17941 cttttaagaa taaaaaaaga gaggcataaa aataaaaatt ttttaattat agatggagaa
    18001 aaatatactt atgaaaatat attgagttat agtgaggaac taggaaagca aatttcttta
    18061 ggtgaaaata tcccaatttt aatatattct aaaaatataa tgtttcagtt gattagtttt
    18121 tttgctatta attattcaaa aaatattcct attatttgtc attataatct ttctaaaaaa
    18181 gtattagatg atattttatt aaaaaataat atttcaatca ttatatctga tgaatctatg
    18241 gacgttattg atttatatga tgataacaat aatgaagtag caaaaaaagt taa
//
LOCUS       DAYAOX010000066        18269 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.066, whole genome shotgun sequence.
ACCESSION   DAYAOX010000066 DAYAOX010000000
VERSION     DAYAOX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.066"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..907
                     /locus_tag="ACGI57_001756"
     CDS             <1..907
                     /locus_tag="ACGI57_001756"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9716836.1"
                     /translation="VTNNRVYGADRHETNANVIKKFYTDDELEAVLVAKSDVLVDALA
                     AGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVYKIGGGLTSKVMSSIASSLSKH
                     NTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVI
                     MTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNKQGHGVEVFYKLKDKNGGTTKT
                     VATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDM
                     SLINSSAKVKEMGTQIGKGIEDSLK"
     gene            1149..3062
                     /locus_tag="ACGI57_001757"
     CDS             1149..3062
                     /locus_tag="ACGI57_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716837.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            3055..5274
                     /locus_tag="ACGI57_001758"
     CDS             3055..5274
                     /locus_tag="ACGI57_001758"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFG9716838.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDNLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            5383..6213
                     /locus_tag="ACGI57_001759"
     CDS             5383..6213
                     /locus_tag="ACGI57_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFG9716839.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            6203..9670
                     /gene="addB"
                     /locus_tag="ACGI57_001760"
     CDS             6203..9670
                     /gene="addB"
                     /locus_tag="ACGI57_001760"
                     /EC_number="3.1.-.-"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HFG9716840.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            9670..13497
                     /gene="addA"
                     /locus_tag="ACGI57_001761"
     CDS             9670..13497
                     /gene="addA"
                     /locus_tag="ACGI57_001761"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFG9716841.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGCRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVNNPDSDY
                     YGEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            13543..14754
                     /locus_tag="ACGI57_001762"
     CDS             13543..14754
                     /locus_tag="ACGI57_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFG9716842.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            14741..18268
                     /locus_tag="ACGI57_001763"
     CDS             14741..18268
                     /locus_tag="ACGI57_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9716843.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKDNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
BASE COUNT         7957 a         1703 c         3245 g         5364 t
ORIGIN      
        1 tgttactaat aatagagtat acggagcaga tagacacgaa acaaatgcaa acgtaataaa
       61 aaaattctat acagatgatg agttagaggc tgttttagta gctaaatcag atgtacttgt
      121 tgatgcttta gcagcaggtc cattggctgc gaacctaaag tctccaatac ttataacacc
      181 aaagacgtat gtatctgcat accataaaga taatttagaa gctaaatcag ctaataaggt
      241 atacaaaata ggaggaggat tgacttctaa ggtaatgagc tctatagcat catcattatc
      301 taaacacaat acgactccaa cagaaccagg aaatagtggg ggcaagacag ttatgattga
      361 cccagggcat ggtggttcag cacctggaaa ttcatctgga ggaatgattg aaaaagatta
      421 caatttaaat acttcacttg caacaactga atatttacgt tcaaagggat tcaatgtaat
      481 aatgacaaga gacacagata agactttatc tcttggaaat agaactgctc tatctaattc
      541 attgaaacca gatttattta caagtataca ttataatggc tcaactaata aacaaggtca
      601 tggtgtagaa gtattttata agcttaaaga taaaaatgga gggactacta aaactgtagc
      661 taccaatata ttaaatagaa ttttagagaa atttaaactt acaaatagag gtataaaaac
      721 aagagtactt cctagtgatt ctacaaaaga ttatttatac gttttaagaa gtaatgatat
      781 gccagctgta cttgtagaat gtgcattttt ggataatgaa aatgatatga gtttaataaa
      841 ctcatctgca aaagtaaaag aaatgggtac acaaataggt aaaggaatag aagattcatt
      901 aaaataaatg gaaataaaaa tatttaaaaa cttaatgtaa atttatagtt gtaaatttat
      961 actaaagtca aaaaatgaaa tataagtgta tcaatagtaa gactgtatgt acatttgcac
     1021 gtgtgaatgc aagtatatat acagtctttt tttgtagtta aatgcaataa tttgaatgtg
     1081 ttttgtaaaa tacacaatat gatataatta gataatatac acttaattta attgaggaag
     1141 gtgattatat ggaatattta tttgaaaaaa gtagtttaaa agaatatgga aatgaagctt
     1201 tagataaata tagagaatta ttagataaaa ctggtaataa ttcaaggtgt attcttttgt
     1261 tagtaccaaa caataacaca aagatacgat atgaaaggtc tctaagatta aattatagtg
     1321 aagaactgaa aataacaaca tatataagtt ttgtgaagaa agaattagtt aaatactggc
     1381 cactaataat agaaaaatgt gatgaaataa gtacaaaggt tgtatctcca acatttatat
     1441 caaatagttt aagtgaatat ttaatagtgc aaaaagttaa ggaaaaaagg atacaagaag
     1501 gatattttga agatattaca ggaaccaata aatccatagc aaatagtata atgactaaca
     1561 taaataagtc tgcatttaat ttaattgatt ttgattttat tggagaaaaa atatattcat
     1621 caaagaaaaa taaagatagt atatatagat tttcatacac tcaaatggat gaaattatat
     1681 catattatat aaattcctta ctatcaaatt caattttaga taatgcatta agcatatatt
     1741 tatacaatca atacctactt aatgatgatt tttatttaaa aaacttattt acagaagtga
     1801 gatatataat tgtggatagc cttgaaagct caagtaatgc agaagttgat tttataacag
     1861 aagcattgga taatacatta caaagttata tatattttga ttatagtagg gattattctg
     1921 tatttaataa tgtggatata gactatataa atgagaaaat aatatcaaaa attaagccta
     1981 aagaagaaaa tattaaaaag aaggagatta gaatagaaga tttatactta ttacctgcaa
     2041 atatagaatt aaatcaatca agtcaattat ataatgaaat gttagattta ataagtgaaa
     2101 aagttatatc tcttatagaa gaagatgtat ctccaagaga tatagcaatt atatctccta
     2161 taaacaatag tatattggag catcaaatta gagattcttt gatagaaaaa aatatagatg
     2221 tttttagtac taagaaggat aaaaaagctg tggattatcc atatgggaat gcattggtag
     2281 tggctacatg tatattttat ggatatttag attttataaa agatgatgaa tacttaagct
     2341 ttttagaaac tcttttagaa ataaatagga taaaagcatt taagattttt aatgaaacaa
     2401 gacatttaag tattgataat atagatgaac atagtcagta tcgtgagatt ttacagtata
     2461 ttgaagaaaa aaagaagagt gatataaaaa tacatgaatt tttaactcaa ttttacatag
     2521 ataaaatgtt aaatttaaaa gaaggtaaaa agaatgttgg gttatgtaaa aagataatag
     2581 ctgaaagtga gtcttttagt gaaagtttaa gtctacttgg gatgaaaaaa gaaaagatat
     2641 ttatagaagc tttaaaaagt acaataaacg attattactc tgttgtcgat atagaagagt
     2701 tgaaatccaa agacaaaata gtaataacta ctccttattc atatatatca tcaaatatag
     2761 atagaagtat acaaatatgg gtagatatag gaagtaatgc ttggaatatg aaaatagaaa
     2821 aagatataag taatttaatt gtacttagaa aatcttttga agaaaaaaag atttatacaa
     2881 atgaaatgga agaatattat aaaaaatact atctatacaa tacagtatat aatttgttat
     2941 taaatgcaaa gaaaatctat gcatttaaaa gtgaatatgc gattaatggt tatatccaag
     3001 agagtatatt atatagtata cttttaaaaa tctcacataa aggagataaa aattatgatt
     3061 aatataaatt atagagaaga ccagatacca ataataaatt acgataatgg tactatggca
     3121 gtaccagcag taccaggtgc tggaaaaaca tttataataa caaatttagt agctaaacta
     3181 ttacttgagc agaaacataa gggtggtaaa atattaattc ttacatatat gaatagtgct
     3241 gtaaataatt ttaaaggtag aataagaaaa attcttgaag agaataaaat agatgatact
     3301 aattcgtatg aagtaatgac tatacacagt ttggctgtaa aaataataaa ggaaaagcca
     3361 gaagttgtaa tgttaagtga ggattttaac atagctgacg atttgcaaaa aagtattata
     3421 ttaaatgaat gtataagtag atttagattt gaaggtggag aaagagcttt tagatggttt
     3481 ttaaaggagc aaaaagatga acgctggaaa gaaataactc ttgaagcctg ggaaagaggt
     3541 ttttttgaat ttgcaggaaa tgcaataagt gaacttaagt ataagggaat ttctccagat
     3601 aaccttgaag aaatattgtc aagaggacac aaaggaatgc ttaaaattat tttaccaata
     3661 tataaattgt atgatagaaa attaaagcaa aatggattgc tagattatga tgatatatta
     3721 attttagcag aaaaaacttt aagcttagac gaagggctta gaaagaagtt tcaaactaga
     3781 tacaaatata tatttgaaga tgagtgtcag gactcaaatg aaattcaagg taatataata
     3841 aaaattatat ctagcgaaaa taaaaaccta gttagagttg gagatataaa tcagagtata
     3901 acaggaacat tttcatcatc agaccctaaa ttttttaaag aatttataaa tagtgcagat
     3961 ttctgttata ggatggatat gtcaaatcga agttcaaaag atatacttga tttagcaaac
     4021 actcttgtca agtatgttac aaaggaattt agacagaaag aatgtagaca tgctcttgaa
     4081 gatatgcaaa taaaaactgt gccaaatgga atgggatata aagaaaatcc taatccagaa
     4141 cattataata taaatgtaaa gtggtatgag agttggaaaa ttgaaataga gcaaactgct
     4201 agatatgtta aaggtataaa aaataaatat ccagataaaa gtataggtat attggttcca
     4261 tttaatgaac aagtgacaca agttgctaaa gaacttagag agaataatct aacttttgaa
     4321 gaattaggac caaattcttt gaataaaagg aaggttataa acaacatagc atatattatt
     4381 gattttatgt tacattgtga tgatgtagaa aagttaatag ttgtacttaa taaagttttt
     4441 atagatactg ataatgaaga agaaataaag tattttttag aagaattaaa aaactattct
     4501 actgaagaaa ttatttataa ttttgaattt agtaagttag ataatgattt aatagaattt
     4561 aaagaagaaa gtgacatata tttaagtttt aaaagaggaa tagatattat aaaacagata
     4621 ttagaatatc caataataag gttagacctc ttagttttgt ttattgggaa aaaacttaat
     4681 ttagaaaaag aagataaagc tgtactggat tatatatctt tttatataaa gtatctagta
     4741 tcagaaaata taaatatgga tttagaagat gtatataatt tattgtttga tgtaaagaac
     4801 agggtgttta atcatataat agatgtggta tatgagataa atggatatga accagagccc
     4861 ggtagtataa cactatgtaa ctatcataaa tcaaagggga tggaatggga ttgtgtgttt
     4921 ttactaggtc ttgttgaata taatttccct gacaatatta atcaaaagtt tcagagtgat
     4981 aaatggtatc taaaagaaaa atataaaaac cctatggcaa ttattaaatc agaagttgaa
     5041 gctatattaa aaggaagtat ttcaacagat tatgctcata aaacgaagat tgattctata
     5101 aatgaaaaga ttagactttt atatgtaggg attactagag caaaggaaat gttagtactt
     5161 tcaggaagtg cttatagaga tgaaagtgat atagggaata agagaaaaga gcaaaaacca
     5221 tgtatatatt taagcagact taatcaacat attattgaaa aaaggtcaaa ttaatatata
     5281 ataagttatt aaaagattgc ttgaaatata tctaaaaggg gataaaacaa tatgtacagt
     5341 aagtaactac taaaaaataa agttaagaga gaagtgatat gagtgaacga taaattaaaa
     5401 tatttttctt atagtcaaaa ttcaattaac acatataaat catgtccatt gaaatttaaa
     5461 tataaatata ttgatagaat aaattggaaa aatgatgatg ttggtagtag agagtattat
     5521 gaaactttaa aaacaggaag agattttcat ttgatttgtg aaagatattt tagcaatata
     5581 cctcttggaa tctattttaa tgaggatgat aaaaatagta aaaagttttt aaagtggatg
     5641 gaaaatataa aaaaagttgt tcctattgaa aaaggaaaaa cgtatttgcc agaatatgaa
     5701 gtaaggatga cattaaatgg ggatataata caggcaaagt atgatttagt tgtaattgga
     5761 aataacaata tagaaatatg ggattggaaa accgagtcta aaaagattga ctataaacac
     5821 gtagaaaata gaatacaaac tattgtttat atgtttttag caaaagaagt aatacctaaa
     5881 gttttaaaaa tggacataaa tgttaaagat ataaatatga gatattatca acctgaattt
     5941 gatgacttac caattaccat tttatatgat gaagaaaaac atgaagcaaa tagaaataaa
     6001 attcaaaatt atataagtat gataaaaaac actaattatg aagaacatac atacgaagac
     6061 aatctataca atgatataga aaaggtatat aataaagaag aacgtatgtg ctatagaaat
     6121 aaaaagcatt gcaagtattg tgaatttaat aagttatgta atggcaaaga aatagattat
     6181 agtattttgg aggcggaaat ttatgggact taggtttgta ttaggtagaa gtggaagtgg
     6241 aaaatccacc tatatactag atgaaattaa aaaagaagct caaaaaaatg aaacgacatc
     6301 aattatactt ttagttccag aacagtacac atttgaggct gaaaatagag taagtaaact
     6361 atttttagga aaagaaaaag ataagtatct aagagttagg gtacttagct ttaagacact
     6421 aagtaatata gttttttctc aagtaggagg attgacagat gtaaatataa attctagtgg
     6481 aaaagcaatg atggtatata gagctattga agatgtaagt gaagaattaa atgtgttttc
     6541 aaaatcaaaa tcgcagtctg gttttgtaag ttcaattaca gatatgatat cagaaatgaa
     6601 acaatacaat ataagccctg aaatgttaga aaatatatct ggagaattgg ataatgaaac
     6661 tttaagttta aaattaaaag atataagtaa aatatataat tcttttgaag ggaaattgca
     6721 tgaaaattat gtagatgctc aagatatgtt aacttcattg gcaagtaaaa ttgaattaag
     6781 cagttattta gatggagctt gtgtttatat agatgaattt acaggtttta caccaaatca
     6841 gtacaatgtc ataaagagta tattaaataa gtctaaaagt gttaatatct ctttgactgt
     6901 agatgatatc aactatattg ggtacagtaa atctgatatg ttttctagaa cgaaatttac
     6961 ttattcaaaa ttgactcaat tgtgcaacga agaaggcata aaaatattgc cacaagtaaa
     7021 tttaaacaca ggtgttataa aaagatttga aaaagttaag gaattacagc acttagaaag
     7081 attttataat gcttatccat ataaaatata ttcaaatcca actgaaaata taaagataaa
     7141 agagtttaac aatttatatt cagaggtgga agagatagcc agagaaattg tacatttagt
     7201 tagagataag aatgtcagat atagagatat aaccattgct acaagagatt taaatagata
     7261 tgatttttta gtacactcta tatttaatga atataatata ccaaacttta tagataagaa
     7321 gagagaggca aaaagtaatc caatagttat tttaattatt tcagctttag aaatgaagaa
     7381 tagacgatat gggtatgaaa ctatgtttag gtatttaaaa agtggactta taggaattga
     7441 taatgatgat ataaatttac tagaaaatta tgtgttggct aatgggatta aaggtaaaaa
     7501 atggtttgat gaaaaatggg attatagaat aacccaaagt ctttcaggtc aagaaagtga
     7561 atttgaactg gaattaaaag agaaaataaa tgaaataaaa aatagagtat tagagcctat
     7621 agtaatttta caagaaaaat taagaggaaa aaatagagta aaagaaatct gtagatatat
     7681 atatgaattt ttattagata taaatatgcc agaaactata gaaagtttaa tagtaaattt
     7741 taaggataaa ggggaacttg atgttgcaaa tcaatactca caagtttggg acatagtagt
     7801 ggacatatta gaccaaatgg tggaacttat gggtgatgaa ataatttctt tagaaaaatt
     7861 tataaaatta ataaccttag gttttgatga atatgaatta ggactggttc ctcctagtat
     7921 agaccaggta cttgtaagta gtgtggatag aatgaaaaat ccagacacga aatatttata
     7981 cctagttgga actactgatg gtgtgtttcc gctgattaca aaggatagtg gaatattgag
     8041 tgataatgat agggaaagtc ttggaaacaa aggaatcgaa gttgatattg atagtaagac
     8101 taggagtttt gaagaacagt ttcttgtata taaagctctt acttcgacat ctaagaattt
     8161 aaccataaca tatccaatat cagaccatga aggtaaaacg cttagaccat caataattat
     8221 atcaagactt aagaagatat ttccaaatat agaaaataaa agttatttag tggaagaaaa
     8281 taaaagcact gataaagata tacttaaaaa aataacggtt aaatccccta catttaatga
     8341 acttataaac gtaataaaaa attatgacag tgatggttac aatacagaag agattaatag
     8401 tatttggtta gatatatata ggtactattt aaaagatgaa atctatagtt ctataacaaa
     8461 aaaagttata aaaggtctta gttatactaa tcaagttcat aagattgaag agaaaaaaat
     8521 aaggtcattg tataaaagta attctcttag catatctagg cttgaaaaat atgctgaatg
     8581 tccatttgct tactttatac aatatggtct aaaagcaaaa aagcgtaaag agtattcatt
     8641 tacaccacca gatttaggaa cttttattca taatatttta gataggtttt caaaagaact
     8701 tttacaagat aatttaactt ggagagatat tgatgaaaaa tatatagaat taaagatagg
     8761 tataatagtt gatgaaataa ttttaaagat accaggatat atattaaata gttctgagag
     8821 gtataaatac cttgcatata gattaaagaa tatgttgaca actgctataa ctataattag
     8881 tcagcaaata aaacaaggtt catttgaacc aatagattat gaagttaaat ttggcaacaa
     8941 tggaaaatat ccacctataa aaatggtgtt agaaaatgga caagaagtaa gtttaattgg
     9001 acaaatagat agagttgatg aatttgaaga aggagaaaat aaatatataa gaataataga
     9061 ttataagtca ggcaataagt ccataagtct aacagaaata tattatggtc tacaattaca
     9121 attacttgtg tatttagatg caatccttga aagtgcaaag gatgaagata taaatataaa
     9181 tccagctgca atactatatt gtagaataaa taatccaata gcaaaattta atgaagataa
     9241 agatgatgaa gaaatacaag aagcaatatt aaaagaactt agaatgaagg ggcttgtagt
     9301 aaaagactct cacatagtta aagagatgga taaatcatta atagatggag aaaggaaaaa
     9361 ctctttagta ataccagtag gtcttactaa ggatggaaat gtaggtaaaa gtacttcagc
     9421 aataagctat gaggatttca aactgcttag aaaatatgta aggcatgcta taaaggattt
     9481 atgtgaagaa atgttaagtg gagaaatacg tatagctcca tataaacata aagatggaac
     9541 atcatgtgac ttttgtgatt actctgctat atgtcagttt gattcaacta taaaagataa
     9601 caagtataaa aatttaaata ataagagtaa tgaagagata ataaaaatga tgaaaggaga
     9661 tgttaattag tgagttctcc taaatggaca aaagagcaat tagaagttat agaaagtaga
     9721 gaatgcaatt tattggttgc tgctgctgct ggttcaggaa aaacagccgt tctagttgag
     9781 cgtataattc aaatgataac aagtagagaa aatccaattg atatagataa gttattagtt
     9841 gttacattta ctaatgctgc tgcgtcagaa atgagagaga ggatagggga tgctatagga
     9901 aaagccttag atgaaaaccc agaaaataaa catttacaaa accaacttgt tttattaaat
     9961 aaatctagta ttacaacaat acactcattc tgtttggatg ttataaaatc aaactttcat
    10021 agaataaact tagaccctaa tttcagaata ggagaccaaa ctgaatgtgc tattttgaaa
    10081 caagaagcaa tagaggaagt atttgaagat ttatatgaag aaagagatga ggggttttta
    10141 aatttagttg aaagttatgc agaaagagga ggagataaag aagtacaaga tattatcttg
    10201 ggaatatatt catttgcaat ggcttctcct gaacctaaaa aatggttgat tgactcagca
    10261 gaacgattta atattgatga aaattttgat ttttcacagt ctatttgggc tagagctata
    10321 ttagacactg taaaaataga gattaatggt ctttgtctaa atatggaaag ggcattaaaa
    10381 gaagtggaat caatagaaga attagaaact tttgcagaaa agttatccgt tgaatataaa
    10441 aaaattgctg acatttcaca agcatgtaat aaatcttggg atgaggcata taaaaagatg
    10501 gcaagtatgt cctttgaaaa ttatgtaaaa ggagtaaaaa gaatttcaaa agatgctcct
    10561 tcgtatataa aagaatcaaa agaaaaggca aaaactataa gagataagac taagaagtct
    10621 ttagaaagta tagtaagtgc tacattcaat aaagataatg attctattag agaagaaata
    10681 aaatatcttt ataacatagt taaaccaata tctagtgttg tactgagatt tgaagaggaa
    10741 tatagcaata agaaaagaga aaaaggaata atagatttta acgatataga gcattttgca
    10801 ttaaatatac ttacagatgt agatgaaaaa ggtaatattg taccttctga tatagcagtt
    10861 ggatgtagaa acaagtttta tgaaatattt atagatgaat atcaggatag taatctagtg
    10921 caggaagtac tgcttaaagc tgttgcaaat acagaaactc caaatagatt tatggttggt
    10981 gatgtaaaac agagtatata tagatttaga caagcaaagc cagaattatt tttgcaaaaa
    11041 tataataact ataatgataa aaagggaagc tcccatagaa aaataatgct ttacaaaaac
    11101 tttagaagta gagaagaagt tgtagatgct gtaaattata tatttgaaaa tataatgaat
    11161 gagaatatag gagaaattga atatacagaa aaagaaagac taaatttagg tgcaaatttt
    11221 aatgtggata cagacgagaa atctattatt ggtggagcaa cggagataca cctaatacaa
    11281 aaagataata aacttgatga tgatattata aatgataaag acgatagaat taataacaaa
    11341 gaaaatgaaa tagaggaaga agaaaaatta gataatatac aacttgaggc tagaatggtt
    11401 ggaaacatta tcaaagattt aatgaaagtc aatgaagatg gaaaaattca aaaggtctat
    11461 gataagggaa tagatggata tagacctgtg gaatttagag atatagttat tcttttaaga
    11521 gctacatctg catgggctcc agtgtttgca gatgaattga tgaatatgga tataccaacg
    11581 tatgctgatg ttggagtggg gtattttgat acgattgaaa taaaaacaat attgtcatta
    11641 cttcaaataa tagataatcc aatgcaagat atacctctta tatctgtatt aaaatcacct
    11701 atatttggat ttacaccaga ggatttgatt gatattagag ttcagagtaa agataaaatt
    11761 ttttatgagg ttttaaaaag tacagcagag tatgatggat ttacagattc tcaaaatgaa
    11821 aatgagtctg agtttatacc aagtgaagag tgcataaaca aaagtaaaga ttttttaatt
    11881 aagctaaagg aatttaaaga aaagtcaatg tacatgagta cagatgaatt tatatggtac
    11941 ttgtacacaa ggactggata ctatgcctat gttggagcat tgccaggtgg aagtcaaaga
    12001 caagctaatt taaaagtatt gtttgagaga gcaaaacaat ttgaagaaac cagtcttaaa
    12061 ggaatattca actttgtaaa ttttatagag aaattaaaaa aatctagttc tgatatggga
    12121 agtgcaaaaa cacttggtga gaatgcaaat gttgttagaa taatgagtat tcataaaagt
    12181 aaaggtctag aatttccagt agtcatatgc tctgcaatgg gtaaaaattt caatactcaa
    12241 gattttaaaa agagtatttt atatcatcat aatttagggt atggacctca atttgtagac
    12301 tatgagagaa ggatttcttt tccaagtata gccaaagaag cattaaaaag caaaataaat
    12361 atagaaaatt tatcagaaga gatgagagtt ttatatgttg catttacaag agctaaagaa
    12421 aaattgatta taactggttc tactagaaat atacaagata gtataaagag atggtcaaat
    12481 ggaattgaaa gtttagatac aatatcacaa tacgagatat taaaaggtaa aaatttttta
    12541 gattggataa tgccttgtgt attaagacat agggatttat ctaatctact agaagaagta
    12601 ggattagacg cagtttttaa tgttgaacat aattcaaaat ggtatggaaa gttgtggaat
    12661 aagaatgata ttttagtgga aaagaagagt gatgaagaaa aagaaagtat tgaagaaatt
    12721 cttgaaaaaa tagatgtaaa taatcctgac agtgattatt acggtgaaat agaagaaaaa
    12781 ttaaactata tatatccata tgaatttagt acaagaaaac ctgctactat atctgttaca
    12841 gaaataaaaa agatacaaaa taattatgaa gaagagttaa taaatactat atttgaacaa
    12901 aaagtaatat taaaaaagcc tctatttatt caaaatgaag aagaaagaga aaaaataagt
    12961 ggaacagaaa gaggtacaat agtccattta gttatggagg ttttagactt aaaaaatgta
    13021 agtagtgtaa atgatataaa atcacaaata agaggttttg tttcaaaagg tataataaca
    13081 gaaaaacaag ctagtatagt aaatccatat aaaatatata aattttttgc ctctaacata
    13141 ggaaaaagaa tgttgaatgc tgaaattata aatagagaaa aatcaattta tgcacaagta
    13201 aatatgaaag acatatatat ttatgaaaag cttataaaca atgacgataa aaaactttat
    13261 gataatgaaa gtgtcatgtt aagaggtata gttgatgctt attttgaaga agataatcaa
    13321 atagttttgg tggattataa aacagatttt gtaaatgagg aaaatataaa tcaaataata
    13381 gagaaatata aaaaacagtt ggatttatat gcagatatta ttgaaacttt aactggtaag
    13441 tcagtaaaag agaaatgtat atatttattt ggagttgatg aagctgtttg ttattagttt
    13501 ttatatataa attaaaccta aaagatagag gaaggggaaa aaatgagatt tattcataca
    13561 tcagattggc atctagggaa gtccctagaa ggccattcta gaattgagga acaagcaaag
    13621 ttttgtgagg aatttataaa aatagtagaa tcaaatgaaa tagatatggt tataattgct
    13681 ggagatgtat atgatacttc taatccccca gcacaagcag aaaaactatt ttatcagact
    13741 gtatcaagac ttgccaataa tggtcagagg tgtgtattaa taatttcagg aaatcatgat
    13801 aatccagaga gactctctgc aataacacct ctagcacatg aacaggggat acttatatat
    13861 ggttatccat taagtgctac gatagaagct aagtataaag ggtttgaaat aacatatgca
    13921 actcagggat gtacaaaact gaatataaat ggagaaaata ttgtggttgc tacattacct
    13981 tatccaagtg aaaaaagatt aaatgaagtt ttttcaagtg aagatgagtt tgagaaacaa
    14041 aaaaattatt ctgaaaaggt aggagatata tttagaagtt tagaagaaaa ttttagaagt
    14101 gatactataa atatagccgt atcacatata tttgtaatag gtggagagag tactgaatca
    14161 gaaagaccta tacagttagg tggaagtttt ttagttgaaa gaaaagattt gccagaaaag
    14221 gctcaatata ctgcactagg tcatttacat aaacaacaaa aagcttccga acgtttaaat
    14281 gcatactatt ctggttctcc acttcaatat agcaaggatg agagagcgta taccaaaggg
    14341 gcatacatag tggatataaa agctggtgaa aaaccaataa ttgaagatgt atattttaat
    14401 aattacaaac ctatagaagt atttaaatgt aatggaatag aagaagcact agatatatgt
    14461 gagcaaaatc aagatagaga gatttggtca tattttgaaa taaatactga tgagataata
    14521 tcacagaatg aaattaaaaa aatgaaggaa ctattaaaag acataataga aataaaacca
    14581 ataataacat cttgttatga acaagaaagt gtagatataa aagaaaagtc tatggcagag
    14641 ttgtttagag agttctattc ttttagtaaa ggtgtagagc caaaaggaga acttatggat
    14701 ttatttctag atataataag tgaagaaggt gaatcagcag atgagaccaa ttagattaga
    14761 acttacaggg cttaatagct atattgataa acaagttata gactttgaaa aattaattga
    14821 aagaggtttg tttgggatat ttggaactac tggaagtgga aaatctacaa tcttagatgc
    14881 aataactatt gctatgtatg gaaatatatc cagaaataca aaagaatata taaacagtgt
    14941 gtgcgataag gcaattatat catacgagtt tgagatagga agcaaaaaca ctagaagaag
    15001 atacattgta gatagaacca ttgctagaag caaaacagga acaaagacct ctaatgctag
    15061 attaatagaa gtattgaatg acaataccca aaatgtttta gctgataaag ttgtggaagt
    15121 aaatgaaaaa gtagcacagg ttgtaggatt aacagcaaat gattttacta ggtcagtagt
    15181 tttaccacaa gacaaattta atgagtttct aagattatca cgtgcagata gaagagacat
    15241 gttagagaga atttttaatc ttgagaaata tggaagaagt cttggagaaa aagtgaaaaa
    15301 gagaaaaaat atacagttac aaaatttaaa agatttgaaa tcaaaattaa gtcaatatga
    15361 tggaattaca gaggaagtat acaacaatat aaaccaagaa ttaatagagt taaaaaattt
    15421 ggaaaaagat aaggataatg ctttagacct agctcaaaaa tcttatgaag aaagtaaaac
    15481 agtatatgaa gaacaattaa agttagaaaa aaatgaactt agaaaaaatg agcttgattt
    15541 aaaaaatagt gagataaaag aaaagattgc tcttgtagaa aatgatagta acgctaaaaa
    15601 ggtagaccca tacataagtt cagttcaaaa tctagaaaaa aagatagatg aagatagctt
    15661 cgcagtaagt aatttagaaa agaaattagc aatattaaat caggagcttg aagttacaaa
    15721 aaatagatat gaaaaaataa acaaaattaa gaatgaagaa gtccctaaat tgagtgaaga
    15781 aaaaataaga ttgcaacaag caataaaatt agaagaagag ttagttttat tagatagaga
    15841 gttaaaagag ctaaaagaaa gtggaattaa tttaaataag actaaagttg aattagaaaa
    15901 agtaaagcag gtgtcagagt ccagaaaaga tgcagttact aaatctataa aagaagttga
    15961 aggaaagatt gataaagtaa atataagtgc agaattaaaa caaaaaatat ttctagcata
    16021 tgaatacgag aaagattaca acaacgtatt ggaagaaaaa aatcaaaagc taaataaact
    16081 agaagaaata ctcaaagaga cagaaaatat aaatttaaaa gttagatata ttgataaaga
    16141 taagaatgat gtaaatcgta atttagaaaa tttatcactt cacttggatg ttcttcttaa
    16201 aaaatgtcct ggtaagagtg ctgatttact tttaaaatct gaatatgtta ctgaattaag
    16261 aaataaagca aataatacta aagaaaatga aattaagaaa agcagtatac aagatgaatt
    16321 aaagataata cttgaaagta agtttaatac tgaaagggaa gtaaatttac taaatgaaaa
    16381 acttgaaaat aatagaaaga acagagatga cttagaaaaa gaattagaag aactaaagta
    16441 tttaaattta gcctctgaac ttagacgtga attaaaagaa aatatgcctt gtccagtttg
    16501 tgggtcaaaa catcatgaaa atcatgatat aactaagtat gatgaaaata tatcttttgt
    16561 caaagaaaaa ctggagaaat tagaaaaaga aaaaataagt ataagaaaca atatagagga
    16621 attaaatgct aaagtaagtg ggtatttatc tatagaaaaa atgaagacta aagaacttga
    16681 agatgtaaaa ggaaaactag gggggattcc atctagtcaa ttattaaaaa aacttgatga
    16741 agaacaaaga aaacttgcat tgttaaaatc taatattcag gagtgggaga aagaaaaaga
    16801 gagtactgaa aataaagtaa cattagctaa agaagaaaaa aataaaattg aaaaagaaga
    16861 attaaaaata agagagagtc tgaataatta taagaaatta acaagagatt taaacataga
    16921 aatagaaggt ttagaaaata aatgtaaaaa acttaaacag gaatacctag gattaaagac
    16981 gattactaaa gtctctaatc tattgtcaaa agtaaaagaa ataagagaga atgaaaaaga
    17041 attagaatta ctaagtactg ggcattcaaa tttattaaaa aatagagact cactagatat
    17101 taaaataaga gaaaatgaga gtaaactaca tgaaatagag atagaattaa taaaggctag
    17161 agaattatat gtagaaaaga aattgagtag agataataag tataaagaag ttattagcat
    17221 aacgaaaggt gatttagcta aaaatctttt acataatgtt gaagaaaata tatgtaagat
    17281 attagaacaa gaagaatcaa gtaagaaaaa attggaagag caaagattag aatatgaaaa
    17341 aaacattgct gaaaagcaca atatagatgg tagacttaaa actgctaagg aacaatataa
    17401 agaccaaaaa tcaacactaa ataaactatt agcagaaaat aaatttgaaa gtatatatgc
    17461 agtaaaaaga gctttattag atactgatgc agtaaaaaaa ttgattgaag aaatactaga
    17521 gcatgaagaa gaacaaaagt tattatcatt taaaataaaa tcatcaaaag aaaaattaaa
    17581 tggaagaagc ataaagaagg agtactttga acagttaaaa gatgaaattt ataatttgaa
    17641 agtagaaata ggaaatatat ccaaagatat tggagctaat caaaatcagt taataacatt
    17701 aaaagactct ttagataaga taaatgattt taataaacaa cttaaggtag ttgaacacaa
    17761 tgtggactta ttggaagaat tagataaatg tgtacaagga aataaatttg tagaatatgt
    17821 ttctacaaac caattaaaat atatagcgtt ggaagcatca aaacgattgg atggaataac
    17881 aaaaggaaga tatgcacttg aaattgactc aacactaaac tttgtaatga gagataactt
    17941 taatggagga gaaagaagaa gcattgatac tctatctggt ggagagacat tcttgacatc
    18001 tctatcatta gcacttgctc tttcatctca aatacaatta aagggaagtg caccattaga
    18061 atttttcttt ttagatgaag gttttggttc attagatagt gaacttttag aaattgttat
    18121 agaatcattg gaaagattgc atagtaataa tctaagtgtg ggtataataa gtcatgtcga
    18181 ggaacttaaa aatagagttc ctgttaagct attagtttca tctagtgagg ctggaatagg
    18241 ttcaaaagta aaaattgaat atagttaga
//
LOCUS       DAYAOX010000067        18083 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.067, whole genome shotgun sequence.
ACCESSION   DAYAOX010000067 DAYAOX010000000
VERSION     DAYAOX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.067"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(12..698)
                     /locus_tag="ACGI57_001764"
     CDS             complement(12..698)
                     /locus_tag="ACGI57_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFG9716844.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(747..1280)
                     /locus_tag="ACGI57_001765"
     CDS             complement(747..1280)
                     /locus_tag="ACGI57_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFG9716845.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(1324..1956)
                     /locus_tag="ACGI57_001766"
     CDS             complement(1324..1956)
                     /locus_tag="ACGI57_001766"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HFG9716846.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2046..3125)
                     /locus_tag="ACGI57_001767"
     CDS             complement(2046..3125)
                     /locus_tag="ACGI57_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFG9716847.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(3258..4604)
                     /gene="pepV"
                     /locus_tag="ACGI57_001768"
     CDS             complement(3258..4604)
                     /gene="pepV"
                     /locus_tag="ACGI57_001768"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFG9716848.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELNKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4673..5953)
                     /locus_tag="ACGI57_001769"
     CDS             complement(4673..5953)
                     /locus_tag="ACGI57_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9716849.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6080..6340)
                     /gene="hfq"
                     /locus_tag="ACGI57_001770"
     CDS             complement(6080..6340)
                     /gene="hfq"
                     /locus_tag="ACGI57_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFG9716850.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6454..7389)
                     /gene="miaA"
                     /locus_tag="ACGI57_001771"
     CDS             complement(6454..7389)
                     /gene="miaA"
                     /locus_tag="ACGI57_001771"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFG9716851.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7427..9394)
                     /gene="mutL"
                     /locus_tag="ACGI57_001772"
     CDS             complement(7427..9394)
                     /gene="mutL"
                     /locus_tag="ACGI57_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFG9716852.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9405..12248)
                     /gene="mutS"
                     /locus_tag="ACGI57_001773"
     CDS             complement(9405..12248)
                     /gene="mutS"
                     /locus_tag="ACGI57_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFG9716853.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIENLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALIKQNDDKEKINQVDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(12408..13142)
                     /locus_tag="ACGI57_001774"
     CDS             complement(12408..13142)
                     /locus_tag="ACGI57_001774"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716854.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(13111..14124)
                     /locus_tag="ACGI57_001775"
     CDS             complement(13111..14124)
                     /locus_tag="ACGI57_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9716855.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(14146..14916)
                     /locus_tag="ACGI57_001776"
     CDS             complement(14146..14916)
                     /locus_tag="ACGI57_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9716856.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(14891..15181)
                     /locus_tag="ACGI57_001777"
     CDS             complement(14891..15181)
                     /locus_tag="ACGI57_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HFG9716857.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(15312..15411)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      16031..16120
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16173..16349
                     /locus_tag="ACGI57_001778"
     CDS             16173..16349
                     /locus_tag="ACGI57_001778"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716858.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(16464..16820)
                     /locus_tag="ACGI57_001779"
     CDS             complement(16464..16820)
                     /locus_tag="ACGI57_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9716859.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            17197..17577
                     /locus_tag="ACGI57_001780"
     CDS             17197..17577
                     /locus_tag="ACGI57_001780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HFG9716860.1"
                     /translation="MTNADGLSESQMQKIARELNNSETAFIFNNLAKNHQENYDVHIR
                     FFTPTNEVPICGHATIAAHYVRAIENQFNNKTVFQKTGAGILPVDIIKKDNDYEIIMT
                     QGKIEVSPPLQQTYQKRLLNAFGI"
     gene            17637..18035
                     /locus_tag="ACGI57_001781"
     CDS             17637..18035
                     /locus_tag="ACGI57_001781"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HFG9716861.1"
                     /translation="MIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHNKETLQ
                     VHGRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPTPNTEFIFEAVQGEAIKRKGKM
                     LVKVIILDNKPVEVKIIGKAVIAFSTNIEI"
BASE COUNT         5791 a         3059 c         1948 g         7285 t
ORIGIN      
        1 ttaaattatt tttaaattat tccaaatacg ccaaataata ttggtataaa tatagctggt
       61 aacaaattgg ctacttttat attcgttaat ttaagtacat ttagaccaag tcctataatt
      121 aataaacttc cagctgctgt catttctgtg actacaggat cacttaaaaa tccatttaaa
      181 aatgatgctc ccacacatat aataccttca tacaaaaata cagatattgc agaaaacatt
      241 acacctatac ctaaagaagc agtaaatata attgaagcta ttccatctat aacagatttt
      301 acaaataaag tatcatgact tcctgtaaga ccactttcta atgaaccaac tactgccatg
      361 gctcctatac agaataataa actagatgat ataaaaccct ctgctataga atctttttta
      421 tttcctttag aaaatttact ttgtaaaaaa gcacccaact tatttaccca tttgtcaata
      481 tctattaatt ctcctatcag agcacctaca gctacggata ttattgtcac taatgtgttt
      541 tttccttcta gtgctcctga tataccaatg tacaatacac ataaggcaat tccattcatt
      601 atagtatcgc ttactctttg aggtaatcct ccttttatta taagacctac tatacatcca
      661 cctgcaattg ctagactatt tgctattact ccaaacattt tttctcccct cgattagcta
      721 attgaaattt atcctattat taaaacctaa acatctatac tttgtattgg atattttaaa
      781 tattccaaaa attcttcaaa accttttcta gatatattta cagttttagt atttatgtta
      841 ggatgaaaat ttaagtaatc ttctttaatt acatctttat ctatgtaaag ctcaactttt
      901 ctttctgtat catttattaa tgaaaaagga tttacacttc caggaagaag tttaagtaca
      961 ttgtatagtc tttcaggtga agcaaacgat aatctagaac ttcctatctt atctttaagt
     1021 gagtttaaat caacttgttt ttctccttta actacaataa gataatactt atttcctttg
     1081 gcatttctta aaaataaatt tttacattga cacccctttg ttattccatc taaaatgact
     1141 aattgctctg ctgtatatac agcttcatgc tctactacat catattcaac atttaattta
     1201 tccaaaatat catatatttt agtttcattc atgctagatt cattttcatc ttgttctttt
     1261 ttattattta aatcactcat acttttctcc ttaaacactt catcataata tcattgtgag
     1321 gtattattct actatagcta ttgctcttac tggagctcca tcagcatcat tatacttaaa
     1381 tggtgctgct ataaatagaa atttttctgg aacattttct aaatttgtaa gattttctat
     1441 gattattttt cctttttcaa ataaaatatg atgcacttca aatactgatg tgtcatatct
     1501 atccactgat agcatatcta ttcctatgcc ttttatatta gtattagcta tatagtttgc
     1561 agcttctttt gttaatgtag gataacctac atagtactgc tttttatccc aaaattttga
     1621 ccaacctgat ttaaatatta taaaatcact attttttatt tcatcttcat atttttttag
     1681 atattctaat tctatattct catttccctt ttcaagttcc aatacataag ctttcccaac
     1741 aaaattttct atatccaact tatctagggt ctcaccttta gtatacatat gtcttggtga
     1801 atccatatga gttccattgt gagaaaatac actaattaat gtttcttgat aaccattttc
     1861 ttcaataata gctactttct tgatatctgg tctattaggc tctgcatata caggcatgtc
     1921 attatgtgtc acatgagtta aatcaaaaac cttcatcttt atataccccc tatgttttta
     1981 ttttctatta tatacctaaa ttcattttat ttctatcaga ttttattcca ttatatacca
     2041 tactactata tattttttat aaacctcaag ggatttaccg aaaaatcttg gaaagaatta
     2101 tcagtaaata taacatcttc acccaaaaaa cgtttatacc ttccttcttc tttctttaaa
     2161 gtaattctct gtttttgtga atgattatta aaaaccatta tcttaagagg aggttttttt
     2221 ctattagtct gcactctttt taacatgtat tttatgtgca tatcagcttc aggaaatgaa
     2281 tatccacaaa atattaaaag gtcagtatct cttaaagttt tttctgtttc attccaaaca
     2341 ttacttaaaa atatgtttga catattctta aaatatgttg gtggcactat aataggttct
     2401 gttaactcac cacactctaa acattttgtc tctgaactat tttcaataag cttcatgaca
     2461 ccaccttcat gtggtgtaag agttacacta ttacaaatgg ggcaataaag ccaattcaat
     2521 gacccatgta tcttatataa tttgattaat ggttcttttg gttttctcca atttaactcc
     2581 tctttaaaat ttgtaaactc cacaccataa tcaagcataa ttggcaaatc attcttatct
     2641 cttaatccag taactgtatt gtctatatgt atatcataat ttgcactaat aaaagtagta
     2701 tcaagaagca aatcattttc caaaagattt tcaactaaaa gtttatgata tttattatct
     2761 gttctaggtg tattatgaat tgcttcagcc attagcagta ttaaatattg tcttaaaaaa
     2821 cgtatactat cacttctgtt atttagattt tctagtccaa aatttctaaa tgcttctttt
     2881 ctttgttctg ctaagtctaa aagacctaaa acttcttcaa aggtaggaaa atttgcatta
     2941 tctatattat cttttactat atcaatattg aacatctgtt taaagaaaat atacaattct
     3001 ctattcattt tggtattata atttgtggga tttagttttt taaaatattc gctaaaaagt
     3061 tcattttgaa taggtagatt ctctgctgct gaagctcctg ctcctaaaaa tattgctatt
     3121 ttcatatagt tcctcctaat ataaatatta ctcctatttt tatatccatt aaatatccat
     3181 atcatttatt ttatgataca acaataaagc tgtcttttac tagaaaaaat cctattacaa
     3241 gacagcctta tattatatta aatctttaat aattcgtaaa tagaaagagc atatatttga
     3301 gtagctttta taatatcctc tactattaag tattcattag cttggtgttc tgtttctggt
     3361 tttcctggaa aaatagctcc aaatgcaaca caattatcta aagctcttgc gtatgttgct
     3421 cctccagatg aaagtggtgt tccatcaaga ccagtttctt cttcatatac ttttctaagt
     3481 gtcttaacaa gaagagtatc ttctggaaca tagattgaat ccaagaaatc atattcttca
     3541 taatttaaat tgtatttatc tgtcattttc tttaactctt ttacaaaatc atcctttttg
     3601 tatgttactg gaatccttac atctattcct gcaaattctt tttcgttatc aatagttact
     3661 ctacctatat ttactgttag tttacctgat acatcatcct tgcagttagg aattatattg
     3721 tttccattag catcttcacc aataacttct gctaaaaact taattatatt agaatctata
     3781 cctatattat taagagctat acaaagtcta gccactgcat ttataccagt atcacttgct
     3841 gctgaatgga cactctttcc aattatacag attttattac cttcaacagt atactcaaaa
     3901 tttaatttat taagttcttt ttttaattta tcagagtatt ttccaatata gattgcttta
     3961 cctggaactg catttaaagc ctttccaact gacaactcta tatcactttt gctatcacaa
     4021 gtcaaatgaa cttgtaaaag acctttctct gcatttgtta ttgggaatct agaatctgga
     4081 gtaaatccat agtttggtat ttcttcatta ttttctttat atttatttat acatctccaa
     4141 agattttctt catcagcccc aaaaatgaat cttatcctct tattaaagtc tacatttaaa
     4201 tccataagag ctttcactgc ataaattgct gatattgtag gtcctttatc atcttgtgtt
     4261 cctcttccat acaatttacc atcttctaaa acagcttcaa atggagggta attccagctt
     4321 tctaaatcac cttctggaac cacatcaacg tgaccaagta tacctatcat tttttcacct
     4381 tgtcctattt ctgcatatcc ataatatcca tctttgtata ctgtcttaaa tcctaaagat
     4441 ttacataaat ctaatatacc tcttaaagct ttatctacat tttcaccaaa tgggcaattt
     4501 tcagttgctt cagatataac actaggtatt tttacacttt cttgtattga agaaatcatt
     4561 tcatcttgta gagaatttac tttttcttta atttgctctt tcatattatc acccttcaat
     4621 tttcatcata atatactaat aaatattttg accttttaaa aaaaaaatat gcttattttt
     4681 tcagtttttt tataaattca atcgctttta aagtccccat ttttgaatgg tcaaatgtaa
     4741 gtccaccttg gaaataaaca ttataaggag gtcttattgg agcatctgca cttaattcta
     4801 tagaagaacc ttgtataaat gctcctgctg ccattatgac ttcactttcg tatccaggca
     4861 tgtcccaagg aacaggtttt acataagaat cgactggtgc agcttcttgt ataccttcac
     4921 aaaaacttat tacttcatca gcattattaa gtctaataca ttgaataata tcacttctta
     4981 agtcatcata ttttggtaat acatcatatc ctaatttttc aaaagctctt gagcaaaaga
     5041 tagctcccat tacagcctga gatactatat aaggcgccaa gaaaaaaccc tgaagtacat
     5101 ttctagttgt tccaaaagta agaccacatt cttttcctat tccaggagaa gtcattctat
     5161 aagatataag ctcaatcaaa tcttttctac cagctatata tccacctgtc aatgcaagcc
     5221 caccaccagg attttttatt aaagagccag ccataacatc tgctccaaca tcagtaggct
     5281 ctttggtatc taaaaactca ccataacaat tatcaaccat tactatagct tctggtttaa
     5341 cagactttat tacttctata gcttctttta tatcactaat tgaaagtgat tttctccaag
     5401 aatatccttt tgacctttgt atcataacga gtttagttct atcatttatt ttgtttttaa
     5461 ttccttctaa atctaagttt ccatcctcta aaaaatctac atcatcatat gtaacaccat
     5521 actctttcaa tgaacctttt tcttctctta tgcctataac tccctctaaa gtatcatatg
     5581 gtctaccagt aacagataaa atttcatctc ctggtctaac tataccttgt atacataaag
     5641 taagtgcatg tgtaccattt acaattattg gtcttacaag agcatcttct gtattaaata
     5701 ctttagaata aatttcctct attttttcac gaccaatatc attatatcca tatcctgtag
     5761 tccagttaaa gtgcatatca cttagtttag attcttgcat agcttttaaa actttatatt
     5821 gattatattc tcttatttct tttatttctt caaacttatc ttttatttct tccattattt
     5881 cttgcgatag tttaaaagta tcatcatcta ttccatagta gtcttttaat aattcttttg
     5941 tctcattaag cattttatca cctgtctctt tattatttta tttaaacatg taaaggagac
     6001 tattttacta aaacaaataa attatagttt ataagttagt ataagtctcc tctcaatcat
     6061 cttaaattaa attaattatc tatctgttgt tattattatt gttgttttgg ttttgatttg
     6121 ttagattaat atactttcca ggttgtatag ttgatacagc atgtttataa atcatgtttt
     6181 gttgtctatt atccccttct attaatatta tataactatc aaaccccttt acaagccctt
     6241 taacttgcac tccattaact aaatatatag taacaggtat cctttctttc cttgcattat
     6301 ttaaaaacaa atcttgtaaa tttaaaactg tatttttcat tagatttgta ccctcccaag
     6361 attaaattga acttctaaaa tattcctacc ttatattcta tataagttat gacaaatttt
     6421 caatattaag ttgtatactt aaaaatttat atattatttt atagaatctt taatatataa
     6481 gattatttca ttttttaatt cgtctatatt ttcatattgg tctaaatcaa accattttgc
     6541 tgttttatat cttttaaacc atgttatctg acgttttgca taccttctag aatctctttt
     6601 aattatttca atagcttctt cgtatgtata ttctccatcc agatacttta atatctcttt
     6661 ataacctata ccttgcattg atatcatatc ttttttaaat cccatactta aaagtttttt
     6721 tacctcttca accaatccat ttttaagcat aatatcaact ctcatattta ttctttgata
     6781 caaatgttct ctatctctat ttagaactat tattattggt tgatattctt cattaaattt
     6841 taaatcactt gaaaaatcat tcattttctt accacttaga caaacttcaa gagctcttat
     6901 gaccctttta gtattgtttt tatggattct attagcagat tcttcatcaa gttcttttaa
     6961 cttattatgc atgtaatcta ttcccttttc agcaagttgt ttttgaagtt cttctcttaa
     7021 ttcattattg gcatcagatt gtgcaaaatc catattatat attaaagagt ttaaataaag
     7081 tcctgtgcct ccagttacta aaacacattt ttccttttta tttatttcat gtatatattt
     7141 ttttgctctt tgttgaaact ctgaaactga aaatggatag tcaggcttaa cttcatctat
     7201 aaggtaatgc ttcactcctt gcatctcttc ctctgtaact tttgcacttc caatatccat
     7261 atattcatat atttgcatag aatcagctga aattatctct gcattcatat cttttgctaa
     7321 ttttattgac aaatctgttt ttccaacagc agttggacct gtaagtatta taagtggtat
     7381 ttttttcata acattccttt ccatatattt taaatatcca aatattttac ataattcttt
     7441 taaacatttt ttctatttct gtcttagata tttcgaccat tataggtctt ccatgaggac
     7501 atgtaaatgg attctcacac ttttctagct gttccaataa actttttatt tcaatatcgt
     7561 atattttatc atttgctttt atagcagacc tacatgccat agatgcaaat ctctctcctt
     7621 ttaaatcata attacttgta atttcttcta tattatcaat tatttgaagt ataaatttct
     7681 cggtttcagg tactccaaat atagtaggaa cacatcttac cattatatga ttattgccaa
     7741 aaatttctag ctcaaaacca aatttcataa ataactccaa gttattttca atttgaagca
     7801 tatctacatt tgatacttca ataaccactg ggtctaaaag tatttgcata tttatatctt
     7861 gtctatagaa cttctccata tatctttcat atagtattct ttcatgtgca gcatgttggt
     7921 ccaacaaata catagaatcg tctttgctca aaattatata agtattaaat acaactccaa
     7981 ttacagaata tccgtataaa gaaaacttgc gttttgaact acacaagtat tcttcttcac
     8041 tgtccttaat actatctcct aagtaataat ttccttcatt tttaattcca tcaacttgaa
     8101 actcttcttg tatattatca tttgcactat cttcacttag ataattaatg ttttttacat
     8161 catcttctac acttgcattt aggacttcac ttactgattg aaattcattt attggttttt
     8221 cacttgataa agtaacaact tcaatgactt catccttacc tttagtaata ttactatttt
     8281 taggagtaga ttctaataaa tcattatttc ttaatttata atctgtactt ttttctctac
     8341 tgttaatagc gattctaggt tgtgtttttt tatcactata agtagcatat tttccaataa
     8401 gatttgagtg tattaattta acctttaaaa aatctctaag ttctatatac acttcttgtt
     8461 ctttttcaaa tttaatttca agtttattgg ggtgtatatt tacatctata catgatgggt
     8521 ctacttctat atttaagaag cacactgcat gtttacctat tggaattata gacttataac
     8581 tttctgttat tgcatctatg attattttac tttttacaaa tcttttattt atatagatat
     8641 gttgtaagtt tttatttgaa cggtatatat tgttattacc tatatatcca ttcattttaa
     8701 agtgattgca cttaaactct acatctatta tattttccgt tatttctttt ccatatatac
     8761 ttctaatagt gtttacaagc tttccatccc ctggagtatt tagcatctgt ttattgttat
     8821 ttgtatattt aaattgaatg tttggattac ctattgcaag tttatttatc aaatcactta
     8881 tatttatagt ttctgcatga gtagatttta aaaatttctg tcttgctggt gtattaaaga
     8941 aaatatcttt tataatgatg gtagttccat tagtagaacc tattggttct ttcgatatta
     9001 tttttccacc ttcaacatat attttagttc ctattatttc atctttagtt ttagttgtca
     9061 tttctaattt tgatacagct gaaatagatg ccaatgcttc tcctctaaaa ccaagagagt
     9121 ataaatcata caaatcatca atttttttaa ttttactagt agcatgtctc aaaaatgatt
     9181 tttcaacttc acttgaaggt attcctattc cattatctgt aatcttaatt agactttttc
     9241 ctccatcaat tatatctatg gaaattttat ttgcacctgc atcaatggag ttttcaataa
     9301 gttcttttac tacagaagaa ggtctttcta caacttcacc tgctgctatc ttatttatag
     9361 tcaaatcatc taggatgtta attatatttt tcatttttac acctctaaga ttttatttct
     9421 tttgcttttc tttgaagatt ataaagagag tttatagcat ctaatggagt catatttaaa
     9481 atatccagat ttaaaatttc ttctgatagt atatctctat ttacactatc aaaagagatt
     9541 tgagttaagg ctacttcttt tacagaatcc acttgattaa ttttctcttt atcatcattt
     9601 tgctttatta aagcaacttc atcatttaat gctttaaatt tagaatttaa tttatcataa
     9661 tcctttttta tatgtttgta gtcttttact ataagttcat gttctatttt tagagattca
     9721 tatttattct taaaattact ttgatttacc ttaactaatt cttcgtccaa attactatta
     9781 attatattat tgttttcttt atctccattt attgcaacac tgttgtaaac atgatttttt
     9841 tccaagtctt taagtatata ttttgctctt tctatgactt cgtctggtaa tttagctaat
     9901 ttagcaacat atattccata actcttgtct gctccctgag gaattatttt tcttaaaaat
     9961 attattcctt ccccatcttc tttgactgct attgaataat ttttaacctc tttaaactct
    10021 tcttctaaat cagtaagttc atgataatgt gttgcaaata atgtcttaca tctaatattt
    10081 ttttgaatat attccactat tgaccatgct aaacttatac catcataggt actagttcca
    10141 cgacctattt catctaatat aactaaactt ctctctgttg cattttttaa tataagggat
    10201 acttcgttca tctctaccat aaaagtagat tgaccttgag ataaatcgtc actagctcca
    10261 actcttgtaa atattctatc taatattgga atatcagcag actctgctgg tacaaatgag
    10321 cctatatgtg ccataagagc tattatagca gtttgtctca tatatgttga tttacctgac
    10381 atattaggac cagttattat gttaataatg ttttctccac gatttaaata agtatcattt
    10441 ggaacgaaat tttcctctcc tactatattt tcgacaacag gatgtctacc atttcttata
    10501 tcaagtttat tattttcatt tattgctggt tttacatagt tatttatatg tgcaactgtt
    10561 gcaagggaca caaaaacatc tatattagct atagtcttag ccactttttg tattctatct
    10621 atgtttttat atattgtatc tcttatctct acaaatatct cgtattctaa agatttaatc
    10681 ttttcctctg catgtagtat cttttcttct atttccttta attctggagt tatatatctt
    10741 tctgcattac taagtgtttg ctttcttata tatgtctcat ctagctttgc ctgtttaaag
    10801 ttagcttttg ttatttctat ataatatcca aaaactttat taaagcctat ttttaatgat
    10861 ttaactcctg ttttttctct ttccctgttt tctatttcct ttacaagaaa ggctccattc
    10921 ttagaaatct ctcttagttc cttaagttca tcactaaaat ctgatttaat tatgtttcca
    10981 tcttttatgg ttatcgtagg ttcttctaga atagcttcat ctattaaatt gtatatatca
    11041 tctaatttat ccatttctga aacatactct tttaggattt ttgcatttga taaatttata
    11101 gtatctttta aattaggtaa cttttctatc gaatttttta agtgaattaa ctcttttggt
    11161 gttactctct caaatgctat ctttccacat attctttcta tatcgtatat atttttaagt
    11221 atatcattta ggtcttctct taatataaag tcatctttta tttcttctat tacatctaat
    11281 ctattttcaa ttttagattt atttatcaaa ggttcttcta catattttct taaaagtcta
    11341 cctcccattg ctgtagaagt tttatcaagt acgtgtaata atgaaccttt tttcttattc
    11401 cctcttatag tttgagtaag ttctaaatta gttctagtaa acatatctag aaccatatat
    11461 tctgatgaat tgtatatatt tatattatta atgttagatg ttatttgttt ttgagtattg
    11521 tatatataat tcagaagaat tgataagctt gactttatta gacctttatc atcaaacttt
    11581 agtttttgta gataaacatc tggaaaatat tcttttaata tatttatatc caaatagtta
    11641 tcactaaaac tttcatttat gtatatatta cttacagtag ctatatctct aagattttct
    11701 ataaagtcta aatcatttat aattatttca gttggatgga tttttgcaat ttcttcaatt
    11761 accttatctt catttaaaca tgttgcatta gtttctcctg tacttatatc aacataagtt
    11821 agacctatat tagtaccatc cttatacaaa gataataaat aattattctt tttattttca
    11881 agtaaatttc catctaaaac tgtaccagga gtgatgactc ttataacctc tcttctaaca
    11941 attccttttg ctgtagatgg gtcttccatt tgttcaccta ttgctacttt atatccattt
    12001 tcaactaatt ttgatatata tgaattggct gagtggaaag gaacaccaca cataggagca
    12061 cgttcttcta aaccacaagc ttttcctgtc aatgctattt caagagcttt tgaagctact
    12121 aatgcatctt caaaaaacat ttcataaaaa tctccaagtc taaaaaacag tatacagtct
    12181 ttatatctat tttttacttc aagatactgt ttcatcatag gagttaattt attcatatct
    12241 atttgcatta tcaaacctcc ttcttttcta attaaatatt atatcatagt agatgattat
    12301 tattaacaca atatataaaa ttttgacata taactaaaaa caatccaaaa tgtatactca
    12361 cattttggat tgctaagttt aatgcttatt aaataaaaat atttttttta caataaagat
    12421 attatttctt ttttaagttt aatgatattt tcaaggttat cttcatttaa ctttctacta
    12481 tctataacaa attctttctt tactattgat ggtttttttg aaataacata aattctgttt
    12541 gatagcatta tagcttcgtc aatatcatga gttataagaa gtattgttga atttactttt
    12601 ttcttaacat ctaaaagcca tttttgcatg gatgttttag ttaatgaatc aagtgcccca
    12661 aaaggctcat ctaaaagcat tatgtcatta gaatttataa aagttctcaa gaagttagct
    12721 ctctgtctca ttcctccaga aagttctgaa ggatatttat cttcatatcc gcttaatcca
    12781 aacacctcaa aataaggttt taccattgac ctagctttac tctttttttc tttttttaaa
    12841 attagtggta gtgacacatt atctattata gttttatatg gcaagagtaa atctttttga
    12901 tacatataac tatacttacc atttatattc actttgccac tatcataatc tgtaagttta
    12961 gttataatat taaaaatagt actttttcca catccacttg gccctaatat agaaacaaac
    13021 tcaccttcat aaacatcaat acttatatct tttaaaactt caatatcttt ataagttttt
    13081 gatacatttt ctatagatac ttttactttt ttattgtggt aaaaattcat ttgtgtaagc
    13141 atcacttgcc ttcatatcct tatctattaa tttatattct tttaagaatg atgtatagtt
    13201 gttccataca ctatctttca tttctcccca tcgtggtgca tcatctttgt acttgcttgc
    13261 taaatattct tgactcttta aagctagttt ttcatctact tcaggtgcat gttttactaa
    13321 aatttttgct gattcttctg gattttttat tgcatattca taaccttttg ttgtagcttt
    13381 taagaatttt ttagcaagct ctggattatc tttaagcaca gtttcattag aaattataag
    13441 tggtgtataa taatccaatc tctcatctaa atcttttact ggtataaagt ttaagtcata
    13501 tccaattaaa tcagctttaa ctgcatccca accttcaaat atccatgcaa aatctacagt
    13561 tttcatagct gcaaagaaat catcttgacc agtattaact atctttaact tattaaaatc
    13621 agctccattt ttcttcataa ctgctttaaa aacagcttct tctgatggag agccccaacc
    13681 accataagtt tttccttcaa aatctttagc tgttgttata ttcttttctt ttggtgatgc
    13741 aaacccagaa gtattatgtt gtataacagt tgctacagct tttattggaa gtggatcttc
    13801 actagtcttt gcataagtaa cttgttcttg atagcttata ccaaagtcag cttttcctgt
    13861 tgctactaaa gtttctgctc cactttcagg tggttgtact atttcaacat ccaaaccttc
    13921 ttctttatag taacccttat caagtgctac aaataatcca gtatgatttg tatttggtgt
    13981 ccaatcaagc accatagtta cttttttaga agaagtatct ttatcattgt ttttgtcttt
    14041 attgttattt gaagaacatc cagttattgt tgccaatgta aaaacactta ctaaacctaa
    14101 tattcctaac tttttaaaat tcataattgc ctccctcaaa tacctttaat ctattaatct
    14161 atgtcttata acaacttttt ttacagcctg tacaattcct acaaacatta aactaaatat
    14221 aactactaat atagtagatg caaatacttt atcaagtgca tatgcacttt ttgacctaag
    14281 catataaaca ccaagtcctt tcgttccacc tagccattct gcaacagttg ctgccattac
    14341 agcatatgtt gcagatattt taagtccaga aaaaaattta tccattgcca taggaaattt
    14401 aaggtgaatg aatgtattaa ctttatttga gtccatagtc ttaaataaat ttaaataatc
    14461 tttatcaata ttctcaatgc catctacaaa acttattaat ataggaaaaa aacaagtaag
    14521 cattaccatc aatacttttg gcattgttcc aaatccaaac catatcataa agagtggtgc
    14581 tataattatt gtaggtatca tctgtgtaac caacataata ggataaagac atttttttat
    14641 tattgataca aaatccatca atattccaat taccaatgat attataattg cgactataaa
    14701 accaagtatt gcttctccta tagtaaccac agcatgcatg gataaatttt gataatcttt
    14761 aaaaaacaca ttaattatat ctactggtgt gggtagtata tactgaggaa cttctaaaac
    14821 tcctaccata gattgccaaa gaataagtat aaacaaaaat gttgctattg gatatatact
    14881 atctttgact ttatctatga tttctaagtt tttcatctat agtcagacct ccagcttttt
    14941 catctactct agctactgtt atgactcttt cacatccaag attaaaaggg attttatgta
    15001 ccttttttaa taattcaaaa acttcatcaa aatcaccttc aacagtagtg ctcattgctc
    15061 ctatttcaca tttaagacca cttttttcaa tttcagcaat acaagcatct actactttat
    15121 acatctcttc ttcacctttg taaggtctta gtggcattac tgctatgtct gcgattacca
    15181 tttaaatcac tcctttaaaa ataattaata cctactaggt atattttgtt ttttattaaa
    15241 aaattttatt actaaaaaga tattatattt aaataaaaga aaagtcttat ccaaaatagg
    15301 ataagactta tttttatctt aactggtctc cctacactgg cattatccaa acaggttcaa
    15361 agggtcgaga taaaatctcc tctcagcaca ttagctcccc gaaatatatt atatcttttc
    15421 tacttgatta tactacactt atcatatttt aacaaatttt ttatttatat tagatatttt
    15481 atccgttcaa aaatatctaa tataaattgt acattattac accaaataaa ttatcttaaa
    15541 atggtatttt atcaacatct tccaaaacta gattaactga attttctagt ttttattttg
    15601 ctatttcaag catatttgga tgtatatcaa ttccatacaa atattgtgct aaatttctgt
    15661 tttcactcat ctaatttgca atcgttacag attacagatt agaaacagtt tttagactct
    15721 acttatcttt atctacaaaa aatctttcaa taaattcatt aataatattc agtaatctaa
    15781 aaataaacct ctatctggat ttttaagata caaacctttt aggaagtatc ttttgtgata
    15841 atataaaaat tactcgtcat tatcatcaaa atcctcagca accaacattt tatataagaa
    15901 tcaaactcta taatataatt attttatcca atttattttt gagaaataaa atattttttt
    15961 aatttttaaa ttttttaata tgcaccttga aaaaagaaac gttttcctat attatggtaa
    16021 tagtagaata aaactaaata ggcaaaacta gagaaattta gtgacgcaaa gctataggga
    16081 ctaagactta tgaaatgagt tatgtcagcc agttgccaaa gagatatttt ctttttgttt
    16141 ttatgaatgt tttttagggg gatatatgat atatgtttat ggataaaatg gatagatgta
    16201 cacatatact aactgcatat atatgcagtt cacgcgatta ctgcaatttt atagatacac
    16261 aattaaatga ttttatacta gaatatggag agaatgtggt agaatcttgt ttgcatcaag
    16321 tgatggtatt ggtaagcaga tataattaaa gaaattttat tttctagaaa acccttttta
    16381 gttttatcta cgttttataa ttcattaaag aactaattaa aataagcttc attatatatt
    16441 tttcatgaag taaacagtta agtttatctc atatactgtt taccccaatt acataattct
    16501 aataatatag attccaatga tttaccacgt ttagtagaca aatattcaac ttttggagga
    16561 atagtcggat aagaaatcct cttaataagt ccatctcttt caagatactt taattcttgt
    16621 gacagtatct tatgacttat atgaacaact tcttttttta attgactgta acgtattggc
    16681 tcttgtttta aaatatgcca taaaataatt gccctccatt tacttcctat cacagtcatg
    16741 gtaaaaccaa ttgaacaatt aaaatctttt tgtctttctt caatatttgg aaattcttca
    16801 aaattattat ctatatccat ctcaatcctc tctttcaaaa tagtaagtat ctaactaaaa
    16861 agtgcattct tgtttagcgt actaattcgt aatataatct tgattaaaaa atagctatgc
    16921 acactcgtga ctttagtcat gggaggttca gtgtagcata gagccttaca taatactact
    16981 tttttagatg tatatgttta aattttaata tagacatatg ttattttatt aattttattt
    17041 aaacagataa taataaataa taatttatct caagttttct atgtagataa actaatctaa
    17101 tgtttagaaa ggattatata atatgaataa aaaatttcgt ctttatcaag ttgactcatt
    17161 acaaaagaaa aatttacagg taatccagct ggtgtaatta caaatgctga tggcttatct
    17221 gaatcacaaa tgcaaaaaat agcaagagaa ttaaataatt cagaaactgc ttttattttc
    17281 aataacttgg ctaaaaatca tcaagaaaat tatgatgtac atatcagatt ttttacacca
    17341 actaatgagg ttcctatatg tggtcatgct actattgctg cccattatgt tagagcaata
    17401 gaaaaccaat tcaataacaa aacagtattt caaaaaactg gtgctggaat attaccagtt
    17461 gatattataa aaaaagataa tgattatgaa ataattatga cacaaggaaa aattgaagta
    17521 tctccaccat tacaacaaac ctatcaaaaa agattactta atgcatttgg tatttaaagg
    17581 aagatattcg tacagattgt cctattgcca tatcatctac aggacattca aaagttatga
    17641 ttggaataaa ggaattatca acattaaata gtttatctcc aaatttatat gaattatcga
    17701 aaataagtca agaaataaaa tgtaatggat attatatttt tacattacat aataaagaaa
    17761 ctctacaagt tcatggaaga atgtttgctc ctgcaattgg tatttcagaa gaccctgtta
    17821 caggaaatgc taatggaccc ttaggtgcgt accttgtaca ttataaagta atacctacac
    17881 ccaacacaga gtttatattt gaagcagttc aaggagaagc aataaaacgc aaaggcaaga
    17941 tgttagtaaa agtaattatt ttagataata agccagttga agtaaaaata attggaaaag
    18001 ctgtaattgc attctcaaca aacatagaaa tctaattata gattccttct aagtaaattt
    18061 ttgaactttt taatactaat tta
//
LOCUS       DAYAOX010000068        17948 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.068, whole genome shotgun sequence.
ACCESSION   DAYAOX010000068 DAYAOX010000000
VERSION     DAYAOX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.068"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            53..388
                     /locus_tag="ACGI57_001782"
     CDS             53..388
                     /locus_tag="ACGI57_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9716862.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            525..1802
                     /locus_tag="ACGI57_001783"
     CDS             525..1802
                     /locus_tag="ACGI57_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein/redoxin"
                     /protein_id="HFG9716863.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1923..3311
                     /locus_tag="ACGI57_001784"
     CDS             1923..3311
                     /locus_tag="ACGI57_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9716864.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEEL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3757..4395
                     /locus_tag="ACGI57_001785"
     CDS             3757..4395
                     /locus_tag="ACGI57_001785"
                     /inference="COORDINATES: protein motif:HMM:NF037435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HFG9716865.1"
                     /translation="MKSKEEIEWLLNRDYKIDPVVTFVGDRYQFSIRQRDALKRAVCT
                     DEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISIEVALSGGTLVIGSDGNIRDLAGLRG
                     TYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDAPVSNSGNLKYRILEHAKTWGIETEV
                     ELVKNADVVLEKLDRVVSSDAVIVDKCISYFNVARGIIEEYIKECNIINLNK"
     gene            4513..5382
                     /locus_tag="ACGI57_001786"
     CDS             4513..5382
                     /locus_tag="ACGI57_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFG9716866.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5537..6682)
                     /locus_tag="ACGI57_001787"
     CDS             complement(5537..6682)
                     /locus_tag="ACGI57_001787"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFG9716867.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(7015..7791)
                     /locus_tag="ACGI57_001788"
     CDS             complement(7015..7791)
                     /locus_tag="ACGI57_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFG9716868.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8373..9347
                     /locus_tag="ACGI57_001789"
     CDS             8373..9347
                     /locus_tag="ACGI57_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HFG9716869.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9445..9750
                     /locus_tag="ACGI57_001790"
     CDS             9445..9750
                     /locus_tag="ACGI57_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFG9716870.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9906..11066
                     /locus_tag="ACGI57_001791"
     CDS             9906..11066
                     /locus_tag="ACGI57_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFG9716871.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNLD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            11116..12018
                     /locus_tag="ACGI57_001792"
     CDS             11116..12018
                     /locus_tag="ACGI57_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFG9716872.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            12098..12991
                     /locus_tag="ACGI57_001793"
     CDS             12098..12991
                     /locus_tag="ACGI57_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFG9716873.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      13044..13225
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13453..14583
                     /locus_tag="ACGI57_001794"
     CDS             13453..14583
                     /locus_tag="ACGI57_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9716874.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTQEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14655..15680
                     /locus_tag="ACGI57_001795"
     CDS             14655..15680
                     /locus_tag="ACGI57_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFG9716875.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            15683..16879
                     /locus_tag="ACGI57_001796"
     CDS             15683..16879
                     /locus_tag="ACGI57_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716876.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(17007..17732)
                     /locus_tag="ACGI57_001797"
     CDS             complement(17007..17732)
                     /locus_tag="ACGI57_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFG9716877.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEYATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSVDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT         6897 a         1997 c         3104 g         5950 t
ORIGIN      
        1 attgctataa aagtttgttt aataaattaa ataaagaaaa gaggagaata aaatgtacaa
       61 catagatgat atgatatata attgtccagt agaagcttta tcaaatattt taggtaaaaa
      121 atgggtggct ataattatat ggaaaataca agaagataaa aaacgatttg gagagctaca
      181 aagagctata ccagactgta gtaagaaaat gttagttcaa caactagatt ttttaattga
      241 acaggggatt atagtcaacc aaaaaaagtt ggttaataat gttgtagagt caacatattt
      301 tttaagtgaa tcaggattac gattgcttcc tgtcatggaa aaaatgatta tgtggagcaa
      361 taaaaattta aattgtgata aatactaaaa tttactctgt aagtaatgaa aatttaatta
      421 agctatgagt agaaaaatgt attaaaatta cataattttt tagttagatt ttaatttaga
      481 aatgggattg taatattgga aataaaataa ggaggaattt taatttgcaa aatgtaaatt
      541 tatttttagt atttatagaa ggtatagtat cattcttttc accatgtata ctgccaattt
      601 tgcctattta tttgagtata ttgtcaaata gtagtgtaga aaatttaaaa gaaggaaaga
      661 cgagttttat aggaagttct ttatttaaaa atactatatt ttttgctctt ggaatctcaa
      721 caacattctt tatattgggt tcatcagtaa aagtattgag tatgtttttt aatgaaaata
      781 aagatttaat aatgtttatt ggtgggatta taattattat catgggatta ttttacatgg
      841 gaataattaa atcttcaata ttgaatagag aaaaaagatt taatgttaag tttaaagaaa
      901 tgaaagcaat aacagctttt atattaggtt ttacatttag ttttggatgg acaccttgta
      961 ttggtccaat attggcatct gtattagtca tggtttcaag ttcaagtaat catcttagtg
     1021 cgaatctttt aatagcagta tatactattg gttttatttt gccattcata attacagcta
     1081 tgttttacag taaactattt aaaactatag ataagataaa atctaacatg gaaattatta
     1141 aaaaaatagg tgggattata cttatagtat caggaatatt aatgatggta aatggatttg
     1201 gaagtataag caaacacttt aatacgtcac aaaatagtaa aattgaaagc aagcaagaag
     1261 aaaataagcg agaaaattct actgataaag aagaaaattc tgatggtaat gatagtcaaa
     1321 aagactctaa taatgacaat aatgataaag ggtctaatga tgaagataga ataaagtcta
     1381 ttgattttac tttaacagac cagtatggta aaacacataa attaagtgat tatgaaggca
     1441 aagttgtatt cttaaatttt tgggctacat ggtgtcctcc atgtaaagaa gaaatgcctt
     1501 atatagaaca actgtataaa gactataata aaaataatga tgatgtagta attttaggag
     1561 tagcttcacc taatttaggt agagaaggtt caagggaaca tgtagttaac ttcttaaaag
     1621 accaaggtta tacattccct gttgtacttg atgaagatgg agcattagca tatcaatatg
     1681 gaattaatgc ttttccaact acatttataa ttgacaagga aggatatgta actcagtata
     1741 ttccaggagc tatggataag gcaactatgg catcatttat tgaaaatcag agaaataaat
     1801 aaaagtattt aagtataagt ttataaatca atttaaggtt acaaaaaagt gcctaattgt
     1861 accacctaaa taatcttgtt atagtaaaag tataaacaat acaagaaagg gttgaatttt
     1921 atatgaaaaa aacatttgat gctataataa taggatttgg aaaaggtgga aaaacattag
     1981 caggggattt ggcaaacagg ggattaaaag tagctttaat agaaaagtca aacaagatgt
     2041 atggtggaac atgtgtaaat gtggcatgta taccaacaaa aagcttagaa aattcagcaa
     2101 acagcgtaaa aacaaagaat ataaattctt gggatgaagt acaagctgaa tatgagaaag
     2161 ctatagataa aaaagaaact ttaattacaa aactcagaga agctaactat aataaattaa
     2221 attcaaatga gaatgtaact atattcacag gaatgggaac tttcattgat gaaaaaacag
     2281 ttcaagtcaa aactgaaaat gaaatttatg agttagtagc agataatata tttattaata
     2341 ctggttcaag accatttata cctaatatta agggaattga aaataagaat atagtatatg
     2401 atagtgagtc tcttatgaat ctaagaactt tacctaaaaa aatgacaatt ataggagctg
     2461 gatttatagg tttagaattt gctggtattt atagttcatt tggagcagaa gttacaatat
     2521 taaactctaa taatggcatt ttaccaaatg aagatgttga ggattcagaa gaaataataa
     2581 aattacttgc aaagagaaat gtaaaaattg ttaataatgc aaatataaaa gagattaaag
     2641 aagtttctga attagcaata gtagaatatg aagtagatgg taaatctaaa gaattaacaa
     2701 gtaatatgat acttgtagct acaggaagaa aagcaaatac tgaggagcta gggctagaaa
     2761 atgcaggtat agaattaaat gaaagaggat ttattaaagt ctcagaaact cttaagacta
     2821 ataaagagca tatatgggct ataggagata taaatggagg tccacaattt acttatatct
     2881 cattggatga ttatcgcata gttattaatc agttgtttgg agataagact agaacaacta
     2941 atgatagaaa gaatatccca aattctatat ttataagtcc agcattttca agagttggtc
     3001 ttaatgttaa acaagctaaa gaaaagggat atgaagtatt agtggcaaag atgccagtag
     3061 aagcaattcc aagagctaaa cagattggca aggcagatgg gtttataaaa atagtgatag
     3121 ataaaaaatc caataagata cttggagcta gtatgatatg tgaaaattca agtgagatta
     3181 tccatcttat tcaattagct gtagatttag aagtagagta tacatattta agagaccgtg
     3241 tttatgcaca tccaactatg acagaagctc ttaatgatat tctatctcct aatatgataa
     3301 aagaagtata acataagttt aatatataaa cctaaaatta tcattataag tagattttag
     3361 aaagaggcta tctcaaaaaa tagagataag tctctttttt actgtttaaa tgataaaaat
     3421 atatttgatt ttataaaaga agattatctt atgaactaaa aagtttattt taaaacacta
     3481 cctatttatg ttataaactt ataaatataa tatagaatct attattctgt attattaagc
     3541 ggggttaagc gttataatta attataaata ttgaataatc taaatggata agataatggt
     3601 attaaaaatt atcttaaaag gtgaaataat ctgtatttaa tgtagtacag ttatatcttt
     3661 aaagcaaggt ggtgaaatga tataaataag atatctaaaa gagggtttga tgatagtgat
     3721 aaaagatggt tttcaagtaa agaattaaca agacttataa agtctaaaga agaaatagaa
     3781 tggttattaa atagggatta caaaatagac ccagtcgtta cttttgttgg tgatagatat
     3841 cagttttcta taagacaaag agatgcatta aaaagggcag tctgtactga tgaaaaaaat
     3901 attataagac aatcaaaaag gctttctcta gataaaatca aagaagatac cgtaaatata
     3961 gatggcttta atttgataat atccatagag gtagcattat ctggtggtac tctagtaata
     4021 ggaagtgatg gcaatataag agatttagca ggtcttaggg gaacctataa gattatagat
     4081 aaaacagaag aagctttgaa tctgataggt aaatttttta ataaatacaa tgcacagaat
     4141 cttaaatttt atttagatgc tccagtttct aattcaggaa atttaaagta taggatatta
     4201 gagcatgcca aaacttgggg aattgaaact gaagtagaat tagtaaaaaa tgcagatgtt
     4261 gtattggaaa aattagatag agtagttagt agtgatgcag tcatagttga caagtgtata
     4321 agttatttta atgtggctag aggtataatt gaagagtata ttaaagaatg taatatcata
     4381 aatttaaata aatagataaa tataggtgag aatatataag tatatgtagc aatattctta
     4441 gtaatacaag taaagtttga aaataggaga acgaatacat atcgaatttc ataaaatgtt
     4501 gtaaaaagac atatgagagg atggatatgt gtgaaattaa ctaaactaaa tattaaaaaa
     4561 tatagagcac ctatatataa atatgcttta gcaaatgtaa atttgaggtc tgctaaatct
     4621 actaattcaa gtatcataac agtaatacct cagggagcca aaatggaagt attggatgaa
     4681 gaagatgact ggattaaggt gatgtacaat tctcaagaag gatatgtgta taaggatttg
     4741 gtatcagtaa gcgaatatgc ttggagtaac ttaaacttaa gagaagataa atctacaact
     4801 tccaatataa ttacagtaat tcctgaaaaa tctagggttg aagtccttca agtagatgga
     4861 gattggagta aggttgtata tgatgataaa ataggatatg tttttaatta ttttttatca
     4921 attgatggga ataaaccaaa tgaattagat tataagtatt tttatactga catgattaaa
     4981 tttgttaacg aaaataacat taaaagtact agcgattatc taattgtaac agaccttaga
     5041 aataaatata cttatatttt taagaaagat aatggaggat ggggacaact ttacaagtgg
     5101 caatgtacta taggcaagcc tgagactcca actataacag gtatatttta tataagtgga
     5161 agaaaacctt cttttggaac ggatgagtat tcagttaagt atgctactag aattaaaggt
     5221 ggatattact atcattctgt actatatgat tcaacaggaa gttatataat agatggcaga
     5281 cttggtgaag ctcttagtca tggatgtata agattaagta cggaaaatgc aaagtggata
     5341 tatgataata taccagatac aacaacagtt ataattcatt aagaaaaatt ttgagttttt
     5401 tattgggata ataattctta aattaataga attatatatt aacttctcat aaagagtact
     5461 atttaaaaag tgtaataaga gagtgctatt aacaaaacaa agatattttg aggcactctc
     5521 ttataaatat acataattaa gatattttca taaacaattc tataatctta tctttatcaa
     5581 agaaaacagg attagcacca gcacaagcat ctttcataga gttttctgca agcaattcaa
     5641 aattaggatt atcaacacct acatctttta aagattttgg gattccaacc tcttcagaaa
     5701 gagcttttat cttatctatt acaaagtcaa cacactcttt atctgattta ttatttacat
     5761 ccattccaat aacttcagct attggtcgga atttggcagg ggctgattta gcattttcct
     5821 cctcaactat tggaagtagc atggcattac atactccatg aggtaaatca tataaacctc
     5881 ctaattgatg agccatagca tgtacatttc caagacctgc attactaaag gcaattccat
     5941 ttaaaaaaca agcataacac atttgttctc tagcttctat gtcattacca tttttaacag
     6001 ctcttggaag gtatttaaat atctgcttta tagcatataa ggcagttgaa tctgtaacat
     6061 cataagcacc attagctact acagcttcta tagcatgagt tagtgcatcc ataccagttg
     6121 ctgctgttaa attagatggt ttgctaatca ttaattctgg gtcatttact gtcattgtgg
     6181 ctagtgaatt ggtatcaacc attatcatct ttacatgtct gtcttcatca gttataacat
     6241 agtttattgt aacttcagca gatgtaccag cagtagtagt tatagctatg ataggaagac
     6301 attttttaga agttttgttg ataccttcat agtctctaat atctcctcca ttagtggcta
     6361 atatagagat agctttacca cagtcttgtg gagaaccacc tcctatagtt ataacgaagt
     6421 cgcaattttc tgattttaat attttaagtc ctttattcac attagttaca gttggatttt
     6481 gtttaacttc atcataaaca acaaagtcta catctatctg agataaaata tctgttactt
     6541 tttttacagt accatttgaa actagaaatt tatcactaac aataaatgct tttttagttc
     6601 ctaatgattt aattgaatct ttagcttcat taagacatcc tttcccaagt aaatttattg
     6661 gtggtacata ataaatgctc ataactaaaa tctcctttta ttttcattga tttttatgta
     6721 aaaatatata tctaacaatt ttcactaact tactcataat atatcgtatc ttagattgag
     6781 ataggattgt tggttcttat ataagaactt aattcgtaat tacgattatg attagaattt
     6841 aacatagtat ttttatcatg tcaataaaaa tataaaaaat attataaaaa aaacaaagtt
     6901 actgtagtta aatcagcaac tttgttttta tagtataaat caaataaata cattagttta
     6961 aaacaaaatt tttaattatt atgttttgat atcttccata tacagaatat taatctaatt
     7021 tattacaatg tcttcattac gttcttccat cattgattga atatttttct gagcatttaa
     7081 gagtaatttt tttatcagtt ctattttttc gtcattagct ctaaataaag gcagtgttac
     7141 actaacagca gctcttatac atccattttt tcttattggt acggcaagac attttaggtg
     7201 ttccattgat tcctcacact ctgaagctat ttgtgttctt tgaacttcaa gaagctggtc
     7261 gtataaaaca tcaaaatctg taattgattt ttctgtaaaa ccttttagac catctggata
     7321 taagcttatt agtttttcct tgtcaaaatc acataaaaga gcttttccaa gacctgtaca
     7381 attagcaggt attttttttc caacataaga aataagtcta attggttctg gagaatctac
     7441 ttttccaata taaagaactt cattattgtc aagtatacca agctgacaag tttcagaaca
     7501 ggaattaact atattagtca tttcgtcaat tatatagtta aaaatagttc ttttttcaaa
     7561 aaatgaagac cctaatgtgt aagaacgtat ccctatagaa tatttggatg tgtttttatc
     7621 aatagtaatg tattttctag cattaagtgt atgaactata ggaaagatgc tactttttgg
     7681 agcattgata acatctgcta tttctgtaag agtatatcca ttttcggatt ctgctaatgc
     7741 ttctaaaata tctaatatcc tagctgttgg gcgatgatta tctgaagtca taaatttccc
     7801 cctcttttta ttaatttaaa gtattagaat atgttgattt tagcacacta tacgatattt
     7861 tacaatgtag aaaataaata tgtaaattta tatataaata agctcttgat ttagatacag
     7921 atagatattt ttttagactg tacattaagg aataactaat ttccaaaatg tacctaaaga
     7981 aagtaagtga tatttagtta tataatttaa gtaaatatat gcaatagtta tttttatatg
     8041 taaatatcaa aatattatga acttatattt tatctatttt ttacactgta taaaaacttt
     8101 atagttttga atactatata attttaaata cgctgtattt ttgcatataa ggaagtcatt
     8161 tgataatata caaggtatta tcagaaagtt tgaaaaaatt ttgacaaaaa tttatttttt
     8221 ttattgactc ataaatagga aaatgctatc attaattcaa agattcgtaa ttaaaaactt
     8281 agttcttaaa tacgaaccga aacggccgtt agaattaaag ctaaaaactt aataagagta
     8341 attaaatatc atgttttagg aggattaact ttatgataat ggattttatt aaaaaaattc
     8401 ctgcaggtat gatgatagtg ccaatgttta tagcagcttt cattaatacc ttttgcccag
     8461 atattgtaca aataggttcg tttacgacag cagtattttc aagtgttggg tctgcaacta
     8521 taattggggt tcaactggta tgcttgggaa ctcagcttca atttagagaa atgccaaaag
     8581 ttttaaagag aggtggtata cttcttggtt ctaaatttgc tataggtgct ttgataggta
     8641 tacttatagg aaagctattt ggaatggatg gtgtccttgg tcttacaaca ttggctgtta
     8701 taagtgcagt tacaaatagt aatggtagta tatatctttc attgatgact tcatatggag
     8761 atgaaacaga ttgtgcaact atgagtttac ttactttaaa tgatggacca ttttttacat
     8821 taatagctct tgggacttca ggtcttgcaa atgtaccatt gttgtcatta ttggctgcaa
     8881 tagtgccaat acttgttggt atgataatag gaaattttga caaaaaaatg aaagagtttt
     8941 tagagccagg gtgtaattta ctcgtacctt ttgtagggtt tgcacttggt agtagcataa
     9001 atcttgcaag tatactaaaa ggaggtttat caggagtatt acttggatta atatctgtat
     9061 ttgtaggagg tatatttata gtattctgtg ataaatttat aggaaaacgt ccaggatatg
     9121 caggatgggc tgtagctaca actgctggaa atgcaattgc tgtacctgct gctgttgcat
     9181 tagttgaccc atcatggcaa ccatatgttg caacagctac aacacaagtg gcagccgcag
     9241 tagttgtcac tgcaatatta gtacctctta taactagttg gtgggctaag aagtatggat
     9301 gtcctaagtt cccaaaacaa aatgaggatg gtacaataac agcttaattt atattacagt
     9361 gagttgttgg taaaataata aattctttaa ttagatttta ggatattaat aaaattatat
     9421 tatttataaa ggagagcaag aataatgata aatgcagtga taattgatga aaaagataat
     9481 gttgcagtag ctattgaagc aatagctaaa ggtagtgaaa ttagttttaa attaaaagac
     9541 aaaacagtaa aaacgattac agcactagat gatataacta tttatcataa attggctata
     9601 aaagatatgg cccaaggtga taaagttact aaatatggtg aacatatagg agaagcaaat
     9661 caggagataa aaagtggtca acatgtacat attcataatg ttagaagtgt tagagaggat
     9721 ttggataaac aagttatgac taatatataa aataatattt atagagttat aaaagttgta
     9781 ttcattgatt ttagtgtcaa atagttttta aataggtttt tagctgtttg attagtgagt
     9841 gtaataaaaa ttataatata taaaaattat agaaagtata tagactattt taggaggaaa
     9901 tcagtatgaa attttatgga tataaaagac cagatggaag agttggtata agaaaccaca
     9961 tattaatatt gcctacgagt gtgtgtgcat cagatacaac tagaataata gcatcacaag
    10021 ttcaaggggc agttaccttt aataatcaga atggatgttc acaagtacat tcagaccaac
    10081 aattaactat ggatgttatg gctggttatg cagcaaatcc aaatgtatat ggtgtaatag
    10141 ttgtatcttt gggatgcgaa aattgccaaa atgatttggt agttgatgca ataaaagaga
    10201 gaacaaataa gccaataaaa actcttgtaa tacaagaaga acatggaact ttaaaaacta
    10261 tagaaaaagc tgttcgttat gctagagaaa tggcacaaga agcttctttg cttagaaaag
    10321 aagaatttcc aatatctgaa cttatactag gaactgaatg tggtgggtct gacccaacaa
    10381 gtggattagc agcaaatcca ttaataggtg aattaagtga taaacttgtc gatttaggag
    10441 caacatcaat tttaagtgaa actactgaat ttataggtgc agagcatata ctagctagga
    10501 gagcagtaaa tgaagaagta aaagaaaaaa tattacatat agtacataga tatgaaaatt
    10561 cattaaaatt ggttggagaa gaagttagag aaggtaatcc atctcctgga aatatagctg
    10621 gtggtttaac aagtttagaa gaaaaatcac ttggttgtat acataaaggt ggtcatagac
    10681 aaataagtga agtatatgat tatgccaaac aaattgataa gaaaggtctt gttataatgg
    10741 atacccctgg aaatgatgct tcatctgttg ctggtatggt agcaggagga gcacaaatag
    10801 tggtatttag tacaggaaga ggaacaccat caggaaatcc aatagcacca gtaataaaaa
    10861 taactggaaa taaaataact tttgcaaata tggaagataa tctggatttt gatgctagtc
    10921 cagttatata tggtccacaa acaatggaag aattaacaga tgatttgtta aatatggttg
    10981 tagatgtagc aaatggaaaa caatcaaaag cagagtccct tggatatact gaaatggcaa
    11041 tagctagagt atgcaactat gtataatctt taagtaagaa aataaataca tagaatttta
    11101 ataaagggtg agattatgaa gtttgtaagt tttactgaag gagaaaatga atgtaaaaga
    11161 actggagttt tttctaaaga tgaattattt attatagatg ttaactcttt aaatctcagt
    11221 agaaatttta aggatttaaa tgaattaatt caaaatgtat ctagtgaaga tattattaag
    11281 ttgaaaacta ttataaatga aagtacattc tataattatg atgtttttaa aagagagaga
    11341 tgtactgtac atgttcctat agaaaaacca atacatgata tcttatgtgt gggagtgaac
    11401 tataaagacc atcttgaaga aactcaaact cactttgatg atagttttga agaaccacaa
    11461 aaaacggttt attttacaaa acgtgtgaca aaggcaatag gtccagaaga tgagatagat
    11521 ggtcattttg agataaataa tcaactagac tatgaagttg aacttggagt agtaataggt
    11581 aaaacaggtg ttaatattag aaaagaagag gtagaagatt atatctttgg ttacactgtt
    11641 ataaatgata tttctgctag aaagttgcaa aaagaacatg ttcaatggtt tagaggaaaa
    11701 ggattagata catttacctc tttaggtcca tgtattttac ataaaagtgc agttcctttt
    11761 cccataaaac ttaaagtatg tagtagggta aatggtgaag aaagacaaag ttctaataca
    11821 gggttattca tatcagacgt ttcagacata gtttcagaac tttcaaaagg tatgacttta
    11881 gaggctggag acattatagc tactggaaca ccttctggag tagggatggg attttcacct
    11941 cctcgatata tgagtagtgg agatgtagtt gaatgtgaga ttgagagtat tggaatttta
    12001 aaaaattaca ttaagtaact gttttaatat aattaatatt aaagctaaat ctgatgtatt
    12061 aatgaacata ttaaaataaa ttttaggagt ttaaattatg aaagcagaat ttttgacacc
    12121 agttgttaca atatttgatg aagaaggaaa tttagataaa gaaggtaata aaaaagtgta
    12181 tgaccatctt atagatggag gtgtagatgg actagtcata atgggaagta ctggagagtt
    12241 ctttaatatg tctatggata tgcaaaaaga acttatagat ttagtaacta gtcatgtaaa
    12301 taagagagta aaagtgttta taggtacaag tcgtatgtgt ataaatgaat ctatagaact
    12361 ttcaaactat gcacataata gtggagcaga tggtgttatg ataataagcc cttattattt
    12421 ctctcttgat gatgatagta ttgagttatt ttatagtgaa atagctaaga atacagaggc
    12481 tgaaatatat ttgtacaatt atccagatag aactggttat gatttaagtc cagaacttac
    12541 attaagactt gcaagaaaac ataaaaacat agttggatat aaggatacag taactttgat
    12601 gggacatacc agagatttaa taaatactat gaaaatagag ttcccagaat ttaaggttta
    12661 tagtggttat gatgaaaact ttatacataa tctaatgtct gggggagcag gttgtatagg
    12721 ggggttatca aatttaattc cagaagtttg ctccaaatgg gctgaagctt acaataatag
    12781 agatatggag aaggttatag aaattcaaaa atatattaat aaagcaatgg atttttactt
    12841 aataaccaat ccatttatac cagcaatgaa aaaagctatg aatatcagag ggttacaaat
    12901 aagtgattat atgactgctc catttgtaaa accaaatgaa aaacaagtat gtgaaattaa
    12961 aaaattgatg aatgaattaa atatatgtta aatcaacctt gcaaagaggt caacaataaa
    13021 ttacaattat cttaataaaa attaaattaa aataatacta gggtactata ttagtttaat
    13081 agggaaagtg atgaaaattc actacagccc ccgctactgt atacggatac gaaatcaaaa
    13141 tccactgaaa tttataaaaa ttttgggaag ggtgatggag gaaaagccgt gagtcaggag
    13201 acctgcccag tattatataa gttcaattta gggtggagat tgtaatttat aataaaatga
    13261 agtatcaatt cgtttttgta aagttgatat atgaacttct ccttattaaa ttaggagaag
    13321 ttttttattt tattaaaagg ttatacttat taaagatgac tttaggataa ctttaataag
    13381 tatactttaa aaagcatata ttattaaagt tttttagcca ctcataaaaa ataagatatt
    13441 ggagtgaaag agatgaaatt taaatcaaat gttttaaagc agtttttttt aattgctatg
    13501 gtaattttta tggtaggagg tcttataggg tgtagtaaaa gtgacaaaga gaataaacaa
    13561 gctagtactg gtaagaaatt agaattaaaa tatgctagtg gatttagtgt tgaagattta
    13621 gaagatggaa ttaaaaaagt aactgatgga gataatcgag aactgatttt aatcccaaag
    13681 aagattaaaa ttcctgaaaa atataaaaat gctaatatag ttagaacgcc tgtggacaat
    13741 gtattaatag catctacaac acaggcttgt tctctaagaa caatagatga actagatagt
    13801 ataaaagccg taacaacaca agagcagtac tggactatta aagaaattaa agatttaatg
    13861 aaagatggaa aaatacatta cgttggttca aatttagcac cagattatga aaaaatagta
    13921 gacttaaatc ctgacttaac tatagtgagt agtggtttat cagaggctga tacaaaattt
    13981 attgacaaat taaaagaatt agggcttaat tatgttgtag acaatgagta taaagagcaa
    14041 aatccatttg gaagaatgga gtggacaagt ttaatagctg cattttatga taaagaagat
    14101 atggctacta gtgaactaaa taaaactgta caaaaggtag aagaagtaag taaaaaaata
    14161 aaaaacaaag ctaaacctaa agttgtatgg gcatctgtat atgaaggaag tgcttatata
    14221 gctcctaaag attcatatgt ggataatatg ataaaaatgg ctggtggaat aaatgcatgt
    14281 gcttcaatag attcaaatga agggcgagta agtattgaac aattgcatga agttgcaaaa
    14341 gatgcagata tatttgtata ctcatcaagt tcagattatg caccaaattt agattctata
    14401 aaatctagtg cacctgtatt ggctgatttg gatgttgtaa aaaacgggaa cctatgggtg
    14461 tttgctccag attattatca atcagtagat aagacagacg aattaatagt agatttagtt
    14521 gagatgttcc acccaggtac tacaggtgaa aaaatcaaac attatataaa ttatgataaa
    14581 tagtacagag aggacacaaa aagataggtt ttatagattt tagttaatat agctttgcta
    14641 aaaggagtat acaaatggat tttatcaata aaatgagaat aaggaatacg acaatattta
    14701 tagtattaat tataagtatg atacttactt ttttagtgtg tgtagcattt ggctctgtaa
    14761 aaattgaatt taaagaagta atagatgttg tattaagagg tatggataat acagaacatt
    14821 ataaaataat atacaatata agaatcccta gagtacttgc aaccatggta ggtggagcat
    14881 gcctagcagt atcaggtatt ttattacaga tattttttaa aaacccaata gttgaaccgt
    14941 atatattagg agtatcttca ggttctactt tatcagtagc tctcatgatt ttgggaggat
    15001 acacattggg atttgagagt gtaaattcaa tgagtatatt tgtagcagct tttataggag
    15061 ctttaattgt aatgtctata gtcattttat ttgcaaataa ggtaaagaat ataacaactc
    15121 ttttaataat aggaattatg accggatatg tatgtagtgg tataacaaat gtgttacaat
    15181 catttgcaaa tagtgataaa ttaaaagaat ttactatgtg gtctatggga agtttttctg
    15241 gatttacatg ggaaaaggta gaaatactta taatagttgg tatattaggt cttttgatga
    15301 ctgtattcat aataaaacct ttaaatgctt ttttactagg agaagagtat gcaaaaagta
    15361 ttggcataaa tataagattt tttagagttt tgatagtatt tatatctagt atattagcag
    15421 caatagtatc agcatttgca ggacctatag ggtttattgg acttgcagtg ccacaaattt
    15481 caaaaatgat atttaaaaca tcagataata gagttttgat accagtagta atatttttag
    15541 gcagtatagt tacagctctt tgtgatttaa tatcaagagt aattttatca ccagtagaat
    15601 taccaataag tacaataaca tctttattag gagctccaat tgtagtaatg ttattggtga
    15661 aaaggaagga tagggcttag acatgttgaa aactaataat ttaagtgtag gatacaataa
    15721 caaagttgtt ataagtaata taaatgtaga agtaaaaaat ggagaaatac tatgtctttt
    15781 gggaagtaat ggagctggaa aaactaccct attaaggtcc ttatctaaac ttatatcacc
    15841 aataaaaggt gaaatatatt tgaatggtgt aaacataaat tgtatatcaa gaaaagcatt
    15901 atcaaaaaaa atggctttag tacttacaaa tagattatta ggtgatttaa tgactgtaca
    15961 agatatagtc aatataggaa ggtatccata tacaggattt tttgggagtc tatctaagaa
    16021 agatttaata atggtagatg aagcattgga gtctgtagat gccttacatt taaagaaaag
    16081 atattttgat gaactaagtg atggagaaaa acaaaaggtg ttggttgcaa gggctcttgt
    16141 tcaagaacca gaaataatta tattagatga gcctacaact cacttggata taaaacatag
    16201 attggaactc ataaacatac tgaaaaaatt aagtaaagaa aagtcaatat cagtaatact
    16261 ttcattgcat gaaatagaca tagcattaaa aagttgtgat aaagttgctc ttataaaaaa
    16321 taataaagtc atagcttatg gccagcctga agatgtagta gatgaggata ttataaattc
    16381 gctttatgaa ttggatgata aaaactttaa tagtctttta ggttcagtag aaattagtaa
    16441 taaatctaaa aatgaagttt ttataattgg tggtgggggt aaagcaacac caatttatag
    16501 agcttttact aaaaaaggta taggtctgta ttctggaata atacatgaaa atgatattga
    16561 ttatgaaatt ggtagaacta tggggataaa gatgtttaca gaaaatccat ttgagcctat
    16621 aagtgatgag agtttcgatt tggctattag aaatttaaat gattccaaga ttataataga
    16681 tacaggattt agtgtaggag agacaaataa gaggaatata gatattataa aagaagcttt
    16741 aaaattggat aaaaaagttt attcttttag aaatagagat gagagtaaga agtattatga
    16801 cagtttggat agtaagatag aacatataga taaagtttct caaataatca atagtgcaga
    16861 tataaataat ttattataat ttatgaaata aaatgtactt atgtgttata gaaaaaagag
    16921 tatagattat aatttttttg aaattacaat ctatactctt ttgttatttt gtttatgtat
    16981 ttatatattt ataaaacttt agcattttat agataaccaa tagcttcctc tctagttaaa
    17041 aaaaagtgaa tccctgtagt cgattctttc catctatttt cattaaaatc ttcagctaca
    17101 atcgtttctc ctactctata tataaaattt tcatcagcat atgcacgagc acttttaaaa
    17161 gattctttac catctacact agtaattgac agtacttttg ccttatcaca tctacaagca
    17221 tttgatgtag cagacactct ttttgcgtcg ctgggtatta ataattgtac cattctaaaa
    17281 ttaaaacatt ttttatagcc tataaatgct cctgtttcag gacaatacaa tttaaaaaaa
    17341 cgagtacttt catctgatat tactccattt aaagtagcat attctaaatt ggaattttct
    17401 aaattagctc ctctaatatc tgagttagat aagtcacaaa atctaaaact tgcaccactt
    17461 aagtctgcat tttttaatat agaatttttt aatgaacaat ccgaaagata agaattatta
    17521 aaaatagagt tttctaaatc tgctttttct aaatttaaat tgataaggtc agaccacgaa
    17581 aagtcaatat tacttaagca gtatcctctt aagtccatat tttctaatct taattctttt
    17641 aaatctgcct tttctccagc attattgttt ttaagccata aataatgttt ctcaagtgtt
    17701 ttttctaatt cttgtttaga aatagttttc attaaaatca ccttcataaa tttattttta
    17761 tatatgtagt agcaaaatag tcatatattt aattatataa caataaataa aaaacttcta
    17821 aataatatat aaaatatcat cttgaaagta cttttttcaa tatatcagaa tatattttta
    17881 agctatttag agataattta aatttttaaa caggacataa taagcttgta ttgttttata
    17941 tttgagtt
//
LOCUS       DAYAOX010000069        17859 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.069, whole genome shotgun sequence.
ACCESSION   DAYAOX010000069 DAYAOX010000000
VERSION     DAYAOX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.069"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            166..876
                     /locus_tag="ACGI57_001798"
     CDS             166..876
                     /locus_tag="ACGI57_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9716878.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKKTVVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
     gene            1000..1143
                     /locus_tag="ACGI57_001799"
                     /pseudo
     CDS             1000..1143
                     /locus_tag="ACGI57_001799"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
     gene            1137..2456
                     /locus_tag="ACGI57_001800"
     CDS             1137..2456
                     /locus_tag="ACGI57_001800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9716879.1"
                     /translation="MLNINVFWIFLPIFSTAIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITMLNTIEFNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADK
                     YIKDINKQIKECNTIFNTQNMILDVILSDKKSICDMNNIYFLVDINFLECNFIEMPDI
                     CSIFSNIIDNAIEACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKVI
                     TSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
     gene            2922..4220
                     /locus_tag="ACGI57_001801"
     CDS             2922..4220
                     /locus_tag="ACGI57_001801"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9716880.1"
                     /translation="MIDSEEMIFNVEDLIIYRKNQLVIIYNKKFRKYIKLSEEIYKYF
                     LLGQENKIKIYDFVNYFDDESDKKYIINLIDIMNKNQIFKSNNLNFDMLSNGVAIYFC
                     LTNRCNLFCKHCCVESSSNKKDLLSTYDLKNIVDIISVLNTPHIIITGGEPLIREDFF
                     EIIKYIKQKLPDTKLILSTNGTLIDEHNVDFIITNFEKIDISIDGVDENTCSETRGKG
                     VFNKVISSVNLLKDNGFSNIKLSMVFGQKNSHLNKQFKDLNKKLGTEFVERYFIPTGR
                     GLENKESYLIEDTVLPITIPYILDNELKMCKDKKNKKMGCCACNAGNSQIFINYDGDV
                     YPCPSLIKDDYKMGNIFDNEFVKVIKENKTRDLKSLIKLESIYPYNFEKCKDCDVNIF
                     CWHCPAILDSVKDNELELDRWCSMMKHNLNTAVWGDEKVN"
     gene            4217..5233
                     /locus_tag="ACGI57_001802"
     CDS             4217..5233
                     /locus_tag="ACGI57_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9716881.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPDID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            5289..5450
                     /locus_tag="ACGI57_001803"
     CDS             5289..5450
                     /locus_tag="ACGI57_001803"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716882.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            5658..8204
                     /locus_tag="ACGI57_001804"
     CDS             5658..8204
                     /locus_tag="ACGI57_001804"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9716883.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            8246..9490
                     /locus_tag="ACGI57_001805"
     CDS             8246..9490
                     /locus_tag="ACGI57_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9716884.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            9538..10527
                     /locus_tag="ACGI57_001806"
     CDS             9538..10527
                     /locus_tag="ACGI57_001806"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFG9716885.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            10564..11238
                     /locus_tag="ACGI57_001807"
     CDS             10564..11238
                     /locus_tag="ACGI57_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716886.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            11222..12964
                     /locus_tag="ACGI57_001808"
     CDS             11222..12964
                     /locus_tag="ACGI57_001808"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716887.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
     gene            complement(13379..14623)
                     /locus_tag="ACGI57_001809"
     CDS             complement(13379..14623)
                     /locus_tag="ACGI57_001809"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9716888.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(14666..17500)
                     /locus_tag="ACGI57_001810"
     CDS             complement(14666..17500)
                     /locus_tag="ACGI57_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9716889.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIMHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
BASE COUNT         7110 a         1733 c         2710 g         6306 t
ORIGIN      
        1 aaatctaaga tatcttctta ttttgttgtt aacatactaa aaataggggg tgatttttta
       61 agtgaataat agtatttgat gaccatgtta gttgtataac taaataatta tttataaatt
      121 gatattttat ttgttttcag atctaatgtc taaggagggg tgtttatgta tagaatagtt
      181 atctgtgagg atgatattac acaaatagct tttttaagag agtgtatatt aaagtcctta
      241 gagggtattt ctagtcaaat tgaactattt gagtttaatt caggagaaga attattggaa
      301 acaaatttag aaggtattga tatatttttt ttagatatta aaatgcttca actaacaggc
      361 atggatgtgg ctaaaataat tagagaaact aatgatactt ctgaaataat atttataact
      421 tcaatagttg attacataca ggaaggctat aaggtaaggg cttatagata tttattaaag
      481 cctatagatt ttggagattt aaatgaaagt attttgagtt gtatatcgga tattataaaa
      541 aaaagagaaa attttatgct gatagaaaat aaaggtataa tcaataaaat tttaattaat
      601 agtattatgt atatagaggt taggaaaaag gttttgacta tacatactaa aaatgatact
      661 tattatacta aaaatagtat ggataaaata gaattagagc tagaaaaata taattttttt
      721 agatgtcata agagctattt aattaatttg gagtatattc aatttatatg taaaaagact
      781 gtggttatta atgatgaaga tgtaccagtt agtaaatata gaatggctga tttaaagaaa
      841 aaattaacat atgtattggg agatgtatta tgttagatat aaatgtattt tggatttttt
      901 tacctatatt ttctacggcg atagaatgaa ttgtattaaa atttttatca aatgaattca
      961 gtgatttaag aaagaataaa tttttaaaaa tgttacaaga gctatttaat taatttggag
     1021 tatattcaat ttatatgtaa aaatactgta gttattaatg gtaaagatat accagttagt
     1081 aaatatagaa tggctaattt aaagaaaaaa ttaacatatg tattgggaga tgtattatgt
     1141 taaatataaa tgtattttgg atttttttac ctatattttc tacggcgata gaatggattg
     1201 tattaaaatt tttattaaat gaatttagtg agttaagaaa gaatagatta accctaaata
     1261 gtagcatagt aattgctatt atgataatta ctatgttgaa tacaattgag tttaatataa
     1321 atattaaatt atttatatgt atatttatta catatgtatt gtataaaata aattatgtag
     1381 tggataaatg gaaaagtgta tttataagtt tgctatattg gatgttatta attggatttg
     1441 atagtattgg attaagtata gtaatgacac ttaattctat aaaaaatatg aatgatttat
     1501 tagaggataa tttgttgaag ttagagttaa taatgatatc taaatcatta ctaatagtat
     1561 taataccatt gcttaagttt atcagattaa aaacaaaaat aaacaagagg gattgtatat
     1621 atttatctat tccattaata gcaaacatta ttggtgtaat agtaatattt gaatttattt
     1681 ttaaagataa gattatcgat tctaaagaga gtttaataat acttacaata tcaactgtat
     1741 ttttactatc aaatctttca ttagtaagca taataggaag aataataaaa gataatagtt
     1801 taaagataga atatgaaatt acaaaaaaaa gaatgaatat gcaatataaa tattatttaa
     1861 atttacagga atatcagttg aaaataagaa aactatatca tgatatgaat aatcatatca
     1921 tttgtattca gaatgtttat gggaaaaatg agtttgcaga taagtatatt aaagatataa
     1981 ataagcaaat aaaagaatgt aatactatat ttaatactca gaatatgatt ctagatgtta
     2041 ttttaagtga taaaaaatct atttgtgata tgaataatat atattttcta gtagatatta
     2101 attttttaga atgtaatttt atagagatgc cagatatatg tagtatattt tcaaatataa
     2161 tagataatgc aatagaagct tgtattaaga ttaaggacac aagtatacaa aaaaagatta
     2221 aattaagagg aactgttata aataggttat ttgtaataaa atgtgaaaat acaaagataa
     2281 atgaagttgt attaaaaaat aataaggtta ttacaagtaa gaaagattcc tttttacatg
     2341 gaataggaat aagtagtgta aaaagttcag tagaaaaata taatggaaat gtggaaatat
     2401 actcagataa aaacaagttt acaattacta tttatatacc attaactagg aactaattct
     2461 agttggggct ctaaattgcc cagttggagc agtagtattg aaagataaaa tagaagatgc
     2521 tatctttaag gcataggata tttggaggtg ataaaaatgg ttgaatgcta tgctaataag
     2581 ataacatcct ttttagtact caataaaata atagaacaaa aaatgtgatt tatatttgtt
     2641 tgaatttaaa atgttgatag gttttatagt tatattattt gcatgatata tcttaaataa
     2701 atttagaaaa atagagttat ttttatctgc aaatgcgacc ttagtatggt aacattattg
     2761 accaaaagtt ctatatagta taaaaaaata aaatgtaaat ttgataaact ttataactag
     2821 gaacataaaa acaacaatta aataactaat tagatagtaa agttgatatt agtaataaat
     2881 attaaagaaa aatattttac taatttgagg aggacttagt tgtgatagat tctgaagaaa
     2941 tgatttttaa tgtagaagat ttaattattt atagaaaaaa tcaactagta attatttata
     3001 ataagaaatt tagaaaatat attaaattgt ctgaagaaat atataagtat tttcttttag
     3061 gacaagaaaa taaaataaaa atttatgatt ttgtaaatta ttttgatgat gaaagtgaca
     3121 aaaaatatat tattaatctt atagatatta tgaacaaaaa tcaaattttt aaaagtaaca
     3181 atttaaactt tgatatgtta agcaatggtg ttgcaattta tttttgtttg acaaatagat
     3241 gcaatttatt ttgtaaacat tgttgtgttg aatcttcttc taataagaaa gatttactta
     3301 gtacatatga tttaaaaaat atcgtagata taatatcagt tttaaatact cctcatataa
     3361 ttataactgg tggagaacca ttgataagag aagatttttt tgaaataatt aaatatataa
     3421 aacaaaaatt acctgatacc aaattaatat tatccacaaa tggaacttta atagatgaac
     3481 ataatgttga ttttattata actaattttg aaaaaataga tattagtatt gatggagtag
     3541 atgaaaatac ttgtagtgaa acacgaggaa aaggtgtatt taataaagta ataagctctg
     3601 ttaatctgct aaaagacaat ggattcagta atataaagct gtctatggta tttggacaaa
     3661 aaaattctca tttaaataaa caatttaaag atttaaataa aaagcttgga acagaatttg
     3721 ttgaaagata ttttattcca acaggaagag gcctagaaaa taaagaaagc tatttaattg
     3781 aagatacagt tcttccaatt acaattccgt atattttaga taatgaatta aaaatgtgta
     3841 aagataaaaa aaataaaaaa atgggatgtt gtgcatgtaa tgcaggaaac tctcaaatat
     3901 ttataaatta tgatggagat gtatatcctt gtccttcact gataaaggat gactataaaa
     3961 tgggaaatat atttgacaat gaatttgtaa aagttataaa agaaaataaa acaagagatc
     4021 taaagtcttt gattaaatta gaaagtattt atccatataa ctttgaaaaa tgtaaagatt
     4081 gtgatgtaaa tattttttgt tggcattgcc cagcgatatt agattcagtt aaggataatg
     4141 aattggaatt ggatagatgg tgtagtatga tgaaacacaa tttaaataca gctgtatggg
     4201 gagatgaaaa agttaattga aatttactat atttgtaact gaagactgca atttgagatg
     4261 tacatattgt tatgaaggta atgataaaaa aaaagaaaat atgagttatg aaactgctga
     4321 taaaacaata gattttattt gtaataaaga attagaaagt aatagtgatt atacaacaat
     4381 tgttttacat ggaggagaac catttataaa ttttgatttg attaaatata tacataggga
     4441 tttagtagat agattgagtg acaaaaagat attatttgat ataactacaa atggaactat
     4501 tataaatgat gaaataatag agtttatagc aaagaatgta aaaaacatat cgataagtat
     4561 tgatggaaaa aagaatatac atgatataaa tagaatagat gctaacggag taggtactta
     4621 tgaaaaagta atggaaaatg gaaaaagact tatagataaa ggtatatatg ttagggcaag
     4681 aatgacatat aactcaaata ctgttaagta tctttatgag agtgtatatg atatagcaaa
     4741 acaagggttt aaaaatatcg caccaatttc agattttatg gataaaaatt gggataattc
     4801 tcatattaaa atattaaatg aacaaattag taaaattatg aatattaaaa aggattatcc
     4861 tgatatagat atatctattt tagataaaca atttataaat aagaaaaaag gagattgttt
     4921 tggagggata aatagttatg caatagatac tagaggagat gtatatccat gtacagtatg
     4981 tgtaggagat aaagatattt tactaggaaa tatatatgat ggattagatg ataaaaaaat
     5041 taagaacctt ataaaaattt atatatctaa gaatgaagag tgtgatggat gtactagact
     5101 tgattattgt atgtcaacca gatgtaaact tatcaataaa aaggtaacag gtaaattcca
     5161 aagtgcagta ccaatttatt gtggattaga aagagtttat gtggaaacaa caaaaaaact
     5221 atgtagttta taatctagtt taactagtta taataaatat aaaacaaaag taaaggggga
     5281 tatgtattat gatacatata aaaaaaatcg aagaaaaaat agaacttcaa gatgttgagg
     5341 tgcaagcaga tccatgtaaa tggtattatg ataagcatgg aataatttca caaatagact
     5401 gtatggtgga ttgtgcagga tcaactccaa aaggaacagt agcttattaa aaaatactaa
     5461 tcagtctatt aaatatgttg aaagtactag atgtagtttt ttacaaatga aatgtgattt
     5521 tctcaactct agtattttta ataattaaat ttaactagca caacgagtta gttagtattt
     5581 aatgaatagt gctctataac ctactatttt aggttataga gtttttcttg taaatatgaa
     5641 taaggaggat tctgattatg ataaatatta aacttgcggt gtcatattta aagagacaaa
     5701 aaggaaaaac tttatcatta atacttagta tagggattgc tgttatgcta gttttttcat
     5761 taagtgttat taatgaatct caaggacaat cagatataaa tcatgcatac aaaaactcgg
     5821 gagattatca tgctttttat gaagatataa atagtgatat agtaaaagaa cttaatggtg
     5881 aagaagatat aaaagaattc aatgatgtac ttaaatttgg agaaattata agtaaagata
     5941 atggagcatc tctaaattta tgctcttaca ataaagattt tttagattct actagatata
     6001 agttagttaa aggtagagag cctagaactt ctaatgaatt agtaatagaa caaaaagttt
     6061 tagacaaaat ggaattagat gtcaaattaa atcaaactat tgattttcag attgttaatg
     6121 agcatataaa taaaaataat gaaaattcta tttatagtaa agataagcaa tttaaaatag
     6181 ttggtatttt aagtaaatct gatgaatact atgaaaattt aaataaatat aaggtaagag
     6241 cttttacata ttcaaacaat ggcaaagata ttgttccata tgaattaata actcatatgg
     6301 gtacattaaa attgaataca aaagatttag attctcaaaa agtagtgaat ctagggagaa
     6361 aatataagtt aacaagtaat caattttatc caaatacaga agtaagtatg tctatgtata
     6421 tgaaaaatat ttcaaaaaaa acaacttatt caataaagca aagcttactc cctttattat
     6481 gtgcaacatt agtaatatac aatatgttta acataataat actagatatg accaagcaaa
     6541 ttggtttact tagagctgtt ggagctagca agagaaatat tagacaggta ttttttattc
     6601 agagtattgt agttttatct gtaggtatag taattggttt acttggaggt atagtttttt
     6661 cttacttagg tctcatgata gtatatgata aaagtgcaga attaactata aatacaaact
     6721 ctataattga atctatcatc atgtcaatta tagcagttat attagcctct gtagtaccaa
     6781 tatataaagc tggaaattta tctataattg atgctataaa aaagacagat aagttaaatt
     6841 atattcctag gcttttgttg ttaaaatcac ataaaaaagt tgttggaatt ataaatgaga
     6901 tagcttttaa aaatatattt aggaataagg ttacatcaat aataataata attacgatat
     6961 cactatgtgg ggttttattt ataggcagac taacttctac aaaattcata tatggaaaag
     7021 agtcagattc agctaatata acaagtaaat catatggcaa ttttgatatt tatttgggat
     7081 attcaccaat aaatgcaaat tatacatttt ctaaatatga taattcttta ttaaatgaaa
     7141 taaaagctat tgaagatgta acagatgttg aacccaatat ttatttaaaa ggatatctaa
     7201 gaaatgctga cttagaagaa gattatttag atgagctaaa aagaactgaa ataaataata
     7261 ataatgagtc tacagtttta attcgaggtt acaataaaga gctattaaat aatatagata
     7321 aatatataga taaaggcgaa aatgtttata gttatacaga cagggattat aaaaatgttt
     7381 tattggtaaa taatttttat tctagaatta aattgtcaaa taatactcag attataaaat
     7441 cacctaaaat aggagactta atagatataa aaattcctgt atataaagat ggagagtata
     7501 aatatataaa tacaaaagta agaatcgctg gaataatgaa aaatagctat atatcagagc
     7561 aagatggaga atctcaatca ggtggaattc aaattatatt taatgaaaag gatttaaaag
     7621 aattaacagg gataagtgat tataataaag tttttgtgaa gataaaaaaa gaaacagata
     7681 aaaaagtaat aaaagaaata aataatataa tagaaaaaag tggattttca gatatagagg
     7741 gaagatatgt acgtaatcat tttggagacc attataataa atcctcatat aaattagcaa
     7801 tgatttgtgt atttggagtt ttattaatat catctataaa cattattttt atagttagat
     7861 caaatataat tgtgaggttg agtgagatat gtacatttag agcaataggg atgtccaaaa
     7921 aaaatgtaaa aagaatgtta ataaaagaaa atatgatata tggggtatta agtatgattg
     7981 tagctggaat tatatctagt ttcaattatt ataaaatagt ttctgaaact aacaaactta
     8041 atcaacaggt atatggtata aataactcta ttaaatttga aattccaata tatgaaattc
     8101 taatatttgg atctatatct gtttttgttt gtgtcattgc agtttatttt tctaataaaa
     8161 tgataaataa acttagtata gtaagtggta taaaagaaaa ttaatgaaat ttctaattta
     8221 aatatatagg aaggaaataa aataaatgaa tagctcaaac aaatattttt tagattgtat
     8281 atgtgaaaaa ataataaata ataatcaagt tattcttagt aataaagtaa atggaagatg
     8341 ggttaaaatt ccaatagaat gttatgaagt tctaaagtat tcagtagata atgcattaca
     8401 acttggtaat ttgtatgatg tattccaaga aaatgaagat aaaaaatatt atgaacgagt
     8461 tatagataag ttagagcata taggacttct aagtgattct attctaagtg aatttagact
     8521 agagagaata aacaaaattt cattttctcc aactaataga tgtaatctaa gttgtgatta
     8581 ttgctgtgta gattctaaaa tagataataa cgattattta gatacatctc aagtaagaaa
     8641 agtaatagat aatatagtta aattaaatcc aaggatatta accattacag gtggagagcc
     8701 attgatgaga aatgatttct tagagctgtt ggattatata aaagaaaaat ttaaaggtaa
     8761 aataattctg tctacaaatg caactttaat taaagaaaaa cagattgatg atataattaa
     8821 tggtttatat gcaatagaaa ttagtttaga tggttatgat gaattaagtt gtagtgaagt
     8881 aagaggtaaa ggagtattct gtaaagtatt aaatgtgatt agacttataa aactaaaaaa
     8941 attcgagaac gtaggtttgt ctatggtggt aggaaagaat aatgagaaag acatagagaa
     9001 attcaataaa ttaaatgaag atttaggtac aacgcctata ataagaaatt ttatgaatat
     9061 tggtagaggt aatgagaact atagcaaata tcttaatgat aaacttgata ttgattacat
     9121 ttgcaaagat gattatgtgg atgaaagtag tttatgttca aatatgtgta aagcaggtgt
     9181 taatcagata agtatagatc ataaaggaga tgtatatccg tgtccaaact tagagtataa
     9241 ggatttaaag atattcaata tattagaatt taatgaaaac atattagaga ggattcttaa
     9301 tagagaaatg gatatattta atatatttga taaattaaaa ccagtaaata ttgagagttg
     9361 taaagaatgt aaagtaaatt tattttgtac aacttgtcca tcgaaaatat atactttaaa
     9421 aaataacaaa aaaatgtttg atgcaaattg taaaaaaatg aaaaacatct tggaacctat
     9481 attatggtaa ggattagttt tatattcaag gtaatttatt ttagaggtga ttattaattg
     9541 actaaagtaa gaatagcagt cttagacaca ggattagatg taaatgatga tgatattaat
     9601 ggaaattatg tttatgattc aaattttcaa ttgaaagatt tgggggggaa ggaaataaat
     9661 gacataaatg gacatggaac ttttgtatcg aaaaccatat taaatataag taaaaatacc
     9721 caaatttatc ctattaatat atttaatgat tctggaaaaa caagttcttt aaatcttctt
     9781 agtacactga gcaagttgac tatgtcggat atacagataa taaacatttc agctgcatct
     9841 attaatagta tgtatgaaaa agaattagaa aaggtttgta atattttaaa tagtcaaggt
     9901 agatttatta tttgttcaaa acataataaa tatgaagata aagaaagtat tcctactata
     9961 tttgaaagtg ttattggtgt agtaggtaat gaaaatatat ataaaaataa tcaatatata
    10021 tacgaaccag aaaataagat tcaaatgtat gcaaattcaa aagataattt tattaaattt
    10081 aaagataaag taactcattt tggtaaaaat agtaaggctt gtgcagtggc aactggcatt
    10141 gttgcaaata tattaaaaaa caatccatat attaagttta atgaattgga agaaatactt
    10201 attcaaaaaa gcaaaagtaa agaagattta aaaatagaaa acttgaacac tccaagttat
    10261 aaatctaatt tgataagatt ggatatagca aggaaaataa taaatgtgat aaatagaaac
    10321 ttttctaaaa taaaaataga ctttgaattt gtaagtgaat attctttatt taacaatttt
    10381 acatgtattg gaaattataa tgcatataat tttttaacta ttttaaataa agagtttaat
    10441 attagtattg actacaaaga agtatactta tatcaattag agaatttaga tatggcagta
    10501 gatcttattt acgaaaattt aatttaaaaa tatatgtttt ttaaatggga atggagatac
    10561 gttatgaaaa tacttaaggt aaaagattta aaaaaaatat atggaaaata tgagaatgaa
    10621 gttagagctc ttgatggtat tgatttagat atagagccac ataaatttac agctattgta
    10681 ggtgcaagtg gttctggtaa aagtacacta cttcactgtg ttgcaggctt agacaagcca
    10741 actttaggga atgtgtactt agatgatgta gatttatata cattaaatga tgataagctg
    10801 tctgaaataa gaagacagga atttggcttc atatttcaaa gttacaattt aataccagtt
    10861 ataaatgtat atgataatat agtacttcct gtgttgcttg atgggaaaaa agaagataaa
    10921 gaatatgtta tgagattaat taataagttg gggattgaaa aacaaataaa gaaatatccg
    10981 aatgaactat caggaggtca acaacaaaga gttgcaatag ctagagcttt agccaataga
    11041 ccgtcagtaa tatttgcaga tgaacctact ggcaacttag atagtaagac tacaaatgaa
    11101 gttatgaata tgcttgaatg gtgtgttaaa gagtacaaac aaacactagt tatgataact
    11161 cataatgata atattgcaaa gacagctgat agaattataa ctataaaaga tggaaaaata
    11221 gatgaaaaaa actattaata ataatttatt tagaatacta aatataatag tatataagaa
    11281 taagactaag ttgatagtat catttataat ggtttttata attgctggcg taaatattgt
    11341 aatacctcaa ctaattagat gtataataga tgatggaata ggaaataaaa atatttttct
    11401 gcttataaat ttaatattga tatatatttt tataaggata ttatcagcta catttgatgt
    11461 tatattatca tatttatata cttcaataaa aaataaagtt actataaatc taaaagtaaa
    11521 gttgcttaac catatatcaa aactttctgg tagatattat tcaagtaaga aaacaggtaa
    11581 tattttatcc attatggaaa gtgatatatt tatgatagaa aactttggag cagagattat
    11641 atttgatatt attgtaaatg tgtttacagc tataatatcg ttaattttct taattaattt
    11701 gcagtttgat ttatttttag tagttttatt tttacagttg atattaacat tttctcaaaa
    11761 taaaatgaca aaacttatat ctaagaaaat ttctgaaata agaagtgatt caggcagttt
    11821 atcaaatttg attcaagagt acgtatctaa tattatggat attgttatat taaattcaaa
    11881 aaagaatttc tttaataagt atttaaagag agagagagat ttgataaaaa aagatttgaa
    11941 tttgaacatc attatgagta gtaatatagc aatatctcaa atactaacag gattgataac
    12001 aatattaata tatggaattg ggggatataa aataataaat aataagatga cgatggggga
    12061 gttaatagca tttcaacaat atacaggaat gttaattgga ccatgtatag ctatagtaag
    12121 ggcgaatact agaatagcac aatcaaaagt gtctatcaat agagtatttt cagtaataga
    12181 tgaaaagata gatattaaag ttgataataa tggatataga atagataaca gtgaaattaa
    12241 gaaaatagat tttaaaaatg tatcttttag atatggtgag gatttaaacc ttgttttaaa
    12301 tgatataaat ttacaatttg agaaagggga agtaacagga attgttggtg gaagtggatg
    12361 tggcaaatca acaataacaa atctaatata ccgactttgg gatataaatg aaggtgatat
    12421 attaatagat ggtgttaata taaaggatat taatttaaaa tcactaagaa ataatattga
    12481 tatagtaact caagatcaat ttgtatttga tgacacaata aaaaataata tagtattaaa
    12541 taataaaata agtgataaag aattagatga gatattagaa gttacttgtt tagatgattt
    12601 tataaaaaat ctagataagg gattggacga agtagtagga gaaaaaggtg tcaaaatatc
    12661 aggaggtcaa aaacaacgta tatcaatagc tagagcttta gtaagtaata aaaaggtact
    12721 tattctggac gaagcaactt cagctctaga taatatttca caaaaagaga tattgaacaa
    12781 cttgaaagag tacatacaag ataagatagt tattataata gctcatagac tttcaacaat
    12841 taagagtgct gataatatat atgtattaga taaaggtaga gtaatagaat ctggaaatga
    12901 tgctgaactt ctacataaag atggtttata taataagtta gttgaaactg cagattttgc
    12961 ataagcatga ctttgtatgt acatatatga ttattagggt atttaatgga atctataagt
    13021 tatataacta atttagttat ataaaagttt gcaaatcaat ctcagataat gttaaacaaa
    13081 tgattagtat atccagtaga aaaattataa tagtagtaga atatattaca aataaatcaa
    13141 aacttcacat aattttcata ttaattatca attaaattca cacacaagag ttattatata
    13201 aacatagaag atagtaaaac tagcttaaaa acataatata ataccctata caaaatagtg
    13261 gcaactctcc ctcacatcct caattgttgc cactatttta attttttgta aaaaacaaaa
    13321 agactccaca aagtggagtc tttataaatt taacataccc ttttaataat atgctttact
    13381 atgctttaaa acaaacagaa tttttaatct caagtttttt atttatctta gcaaccttaa
    13441 tcttaatctt atccttcaaa ggaatacatc ctatattact ttcacttttt acatcaacac
    13501 ttccaacaaa tttaccagag tcattataat actttgcaac accaacaata tctcccttat
    13561 ggataggata attcatattt ttaaacacta ccttaggatt gatattttcg ttatctgaaa
    13621 caacaccata caaatcttta tcaaccttaa ctgttatctt aaaatcatca ataccatcaa
    13681 cacaaggagt ctctataata tcacccttat gtgctatctt tttagaatgg aaattatctt
    13741 taccatattt caataaagta gcggaagaag aaattctatt tttcttgtta ccatcaccaa
    13801 gtacaacacc tatcaatcta tgatttcttt cgtttttagc atctttaggt accatcatag
    13861 aaaatgcaag gcaatatcca gcattatctg tatatcctgt ctttattcca tcaacaccag
    13921 gtactgaaac aagcagagga tttgtgttat aatgtgtaaa atccttttta gtatcattat
    13981 atacttgcat agtggttatt cttgtaactt gattttcata atggtcgtac atatactttc
    14041 caagtgtaac tatatcgtga gcagttgaca tattttcaat aggaggttct ttatgttctg
    14101 ggtcagtata tataggaagc ccatttggat taaaataata cgtatcaatc attcctattt
    14161 cttctgcttt tttgttcatc atatttacaa aattcttttc agttttacca atatgtttag
    14221 ctatggctat agcagcatca tttccagata ctagcattag accctgcata agctctccta
    14281 gagatatttc atcaccatct ttaagtttac aagtagaacc tcctactgaa gctgtagact
    14341 tgtctatttt caccatatca gtctctttaa cttgattttt atcaactgct tctattgcca
    14401 ataaaaaggt catcatttta cttaaactcg ctaatggcat tttttcatca atgttttttt
    14461 caaataatat tcttttagtt tcctggtcta taagtagtgc tgctcttgaa gaatttggta
    14521 ctggtggagt atctgcaaat gcaaatacag gagttataaa tataaaaatt aatgcaagta
    14581 ttgttaactg ttttattttt cctttcaaaa tctcgtcctc catttcatat attaattaat
    14641 atgcacaaaa taagtgctat aaattttatt tatttagata ctcattaatc atgtctatta
    14701 tataagcaat ttcttcgtct ggtatcaaaa tattaaaata agcttctgtt ggcttaaagg
    14761 cttcacgtat taaactatat agttcatgat ttttactaat taattcagta atgttattat
    14821 atggaaaagt tgtattttta aaacatcttt ctatcataaa agagcaatgt agtatatatc
    14881 ctagcttaaa tttgctcttt aagttttcat ctattaattc tattattgta aataaggaag
    14941 aattgacata gttgaataat ttaacagagt ccaaaagctc taagttttgg gtcataaagt
    15001 ttattattaa actgttgtat gatagatttg tttcttttat atttgtaata ctattggcat
    15061 tgaatgtacc ttctattatt ttgtttagca tatctaggcc atatccattt agtagttcct
    15121 ctatgtgtat aaaaggggtg ttggctacat taaaatctac acttccaaca actgccagaa
    15181 ttttagtatt actaaattta ttcttataca ttttaaaact ttctatatta tgaggaacta
    15241 aattaatatt gtatttacta atatttttaa gtgaagaatt tataaagtct gctactttca
    15301 tagctgaacc atatcctgtt atacaagtag ttattattac atactctctt gacttttcag
    15361 gatagtttat ttctgttgaa tcgattgctg ctcgtccaat atatggattt aaagagctta
    15421 aggaattagc taattcatcc aaagtcattt ctggtagtgt actttttctt actgcttcta
    15481 aaactattgg agtagatacc atttcaattg agcgagtttt gatttttgtt ttttctgaaa
    15541 taagttctcc aaaagctata agagagccca tatctgttaa aagaagtaca cccttacctt
    15601 cgtcacattc ttttaccaac tctatagttt tatcaagtat ggtacttact tttacatcaa
    15661 gaggcatgtc aattgcatta caatggtttg taccaagcaa tgaatttgct acatcacaca
    15721 tactgcttgc tgttgaattt ccatgagcaa gtattatgac tcctatactt tcagattgat
    15781 tgtttggttc aagatacatg ctatttagaa acatggatat aaattttact tcatctttag
    15841 gtaaagatgt ttgtaagtca ttttcaatta tacttaaaac tattttagct atatttaaat
    15901 ctttatctaa gaaaatttta tcattatata aattgtactt aggattatat gtgtcttgat
    15961 ttatattttc tataagtgca cttatatgca ttgaaagagc aatataagtt tgttttgtgt
    16021 atttgtttct cgatattttt tcagcctctt ttaaagaatg ttcaacagca tagtaaatct
    16081 ttggattgat tactttaaaa atctcattac ttgtataatc agaatgagtt actgaagata
    16141 tcgaaagtaa ttttgtagag tacttttcca tgtaatttat aaacatactc cttatatcat
    16201 tttccagata aggaatctct gtagagtatt taaatccatt gtttgatgta tctatattgt
    16261 atttagatag ttcttttgta tattcaactt ctaagttatt accattaaat tcataaaaaa
    16321 gcatataatc aagttctgat aatttattta gattactacg attttcattc aattttaaaa
    16381 gaccaatata gatgtaatca ggaacaaggg ataaatctat ttctatatta tccttaagtc
    16441 cagttttgta atttaagaag gctcttgcac tcataagttg tatgtcagat ttaagctgac
    16501 ctatattacc aaaacaatca taaaggagta atgatttaat tacatctttg tgaactttta
    16561 tagttgcatt ggtgtatttt gattctataa taaagaagta tttaataaga gataatcttt
    16621 cttttaaaga cctatcctgt aaagcaggta ggttgattgt aacagggatt cttcttaaaa
    16681 aagtcccaag cagagaagaa tgtatatctt ctgttgtggc acacacaata aagacattag
    16741 ctttgtgcat aatgtcagat tctcctaatc gtctgtatat gcctttgtcc atcaaaagaa
    16801 aaagcatctc ttgaccttct ggtggcaatc tgtgtatttc atctaagaat aatatgcctc
    16861 catcagcctg tgcaactatg ccgaatttat ccttattagc accagtaaaa gaacctttga
    16921 cataaccaaa taaattactt aataataagt tagcattttc tgcatattca gcacagttaa
    16981 atactacgaa tttgctttca gaagaaaaga ttttactttc tacagcatat ttgtacattg
    17041 tttcagcaaa tgtacttttt ccaacaccag taggtcctat tagtaacgta ttaagacctt
    17101 ttggaggata gagtatagca gatttagctt tttctatagg tagtttaaga ctagaatcat
    17161 gtccaagtaa tgttgaaaaa atatcttctg agtcattttt acttgattta agtagctcat
    17221 tacaattatt tatagaaaat tcatcactgt tcaaagtacg gccaattaaa ttttctataa
    17281 tactagcatg tagaaataaa attggcttac cagatatttt cagaactttt ttttcattgt
    17341 ataggttatt taattcttta ctggtattac ttctatcaag agatgaatgg ttagcaattt
    17401 ctattgtagt gaatcctgtt ttatctgatt gaaggtcttt taaagtcata ttttcacata
    17461 atttatgtag agataataga actttatctt ttcttagcat ataccgtttc ctttctttat
    17521 gtttaaacta tatttaagct acattaaata tattataata tatattcagt attaaaataa
    17581 ggtttatagt ggatattttg tattttactt tttaatatgt tgttttgtgt aatagatggt
    17641 gttaaaataa gagttgcatt atattaagta tggattttta tagattaaat tagagtatta
    17701 tgttgttaaa taactaaatt taagtttttt aatatactta agtctatgta atatcattta
    17761 ttaatatatg gttggtatag atgaagatat atgaaaaaac tgataaggtc aagaataatt
    17821 tatatataca cagttaatac atatatacat ttacaaatt
//
LOCUS       DAYAOX010000070        17851 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.070, whole genome shotgun sequence.
ACCESSION   DAYAOX010000070 DAYAOX010000000
VERSION     DAYAOX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.070"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            429..3974
                     /locus_tag="ACGI57_001811"
     CDS             429..3974
                     /locus_tag="ACGI57_001811"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9716890.1"
                     /translation="MNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHTY
                     LSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRMT
                     ISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKSG
                     KIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQLF
                     DKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKIV
                     SIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGGV
                     KTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQLE
                     KQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSKR
                     FEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFKD
                     DRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVDI
                     TSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENAT
                     KNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDNN
                     GKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKGA
                     LMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDIS
                     ETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALYS
                     AKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIEV
                     AVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLGN
                     YLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSYN
                     RMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTYE
                     LLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKFE
                     VQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            4159..6093
                     /locus_tag="ACGI57_001812"
     CDS             4159..6093
                     /locus_tag="ACGI57_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFG9716891.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6199..7194
                     /locus_tag="ACGI57_001813"
     CDS             6199..7194
                     /locus_tag="ACGI57_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HFG9716892.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7166..7951
                     /locus_tag="ACGI57_001814"
     CDS             7166..7951
                     /locus_tag="ACGI57_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9716893.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7939..8637
                     /locus_tag="ACGI57_001815"
     CDS             7939..8637
                     /locus_tag="ACGI57_001815"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716894.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8760..9233
                     /gene="ybaK"
                     /locus_tag="ACGI57_001816"
     CDS             8760..9233
                     /gene="ybaK"
                     /locus_tag="ACGI57_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFG9716895.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKFYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9430..10269
                     /locus_tag="ACGI57_001817"
     CDS             9430..10269
                     /locus_tag="ACGI57_001817"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFG9716896.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKN
                     VVI"
     gene            10282..10830
                     /locus_tag="ACGI57_001818"
     CDS             10282..10830
                     /locus_tag="ACGI57_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HFG9716897.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10896..12077
                     /locus_tag="ACGI57_001819"
     CDS             10896..12077
                     /locus_tag="ACGI57_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HFG9716898.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12369..12758
                     /locus_tag="ACGI57_001820"
     CDS             12369..12758
                     /locus_tag="ACGI57_001820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9716899.1"
                     /translation="MMEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNIT
                     AYDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQ
                     PGVEAFYEKNGCQRSLQSYIIKKKDSY"
     gene            12908..13171
                     /locus_tag="ACGI57_001821"
     CDS             12908..13171
                     /locus_tag="ACGI57_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFG9716900.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13300..14142)
                     /locus_tag="ACGI57_001822"
     CDS             complement(13300..14142)
                     /locus_tag="ACGI57_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HFG9716901.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14437..15171
                     /locus_tag="ACGI57_001823"
     CDS             14437..15171
                     /locus_tag="ACGI57_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9716902.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            15181..16314
                     /gene="nagA"
                     /locus_tag="ACGI57_001824"
     CDS             15181..16314
                     /gene="nagA"
                     /locus_tag="ACGI57_001824"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFG9716903.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16413..17162
                     /gene="nagB"
                     /locus_tag="ACGI57_001825"
     CDS             16413..17162
                     /gene="nagB"
                     /locus_tag="ACGI57_001825"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFG9716904.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17337..17609
                     /locus_tag="ACGI57_001826"
     CDS             17337..17609
                     /locus_tag="ACGI57_001826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716905.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
BASE COUNT         7143 a         1875 c         3142 g         5691 t
ORIGIN      
        1 tttgagtatt aatctaaagt atatgttttt atatggttta aattttatgt atacaaaatt
       61 aatttgctaa ctgaatcata taaaaaaaaa caaaatattt tttagaggga atgattagat
      121 agaatttttt tgttatgaaa ttaattgaag agtaattaat tagaaaagga atgaataata
      181 atgaatatat tacattatta ctttttgata aatcaaaaat aaagttataa tttttaatat
      241 taattaagaa tgatgaaata tgaataaaga ttttacaaat aaatcttaag ctatcttatt
      301 taattctaat acaggaagaa ggtaaatgta tattgaaaaa atcatagaaa tagaagtaaa
      361 caatttaaat aaatacaaag gtttgcaaaa gaagtaatat aattatatac aatgaggatg
      421 tgagtatgat gaacagacta aagccaagaa ttttaggtat tagtatcttg tttattatta
      481 taattatttt aacatgtgga gctgttttca agtttaaatt taaattagat aataaattaa
      541 aagaaaccac acatacatat ttgtctgagt ttacagaaca caatataatt aatctcaaga
      601 caagacttag tggtcaattt gacatgcttg aatctattgc taacttcata ggaaacttga
      661 ataatataaa tgatgaaatg gtgattgatt taatggattc tgatgtaaag cgaagttctt
      721 taattagaat gactatctct acattagatg gggaatgtta ttctaatgat ggaattaccc
      781 ataatgttca agatagagat tattttaaaa aagctaaaaa tggagaaaag aatatatcag
      841 agcctttaaa atctattgtt aataataaag aaataatagt tttatctacc ccagtttata
      901 aaagtgggaa aatagttggt gttttaagtg gaatttatga ttcaaaaaag ttaaatgagt
      961 tattaggatt atctgcattt gatgaaaaag cagatgtttt tgttgcaaag aaaaatggag
     1021 atatagttgc tcaaaaaaca aataaaaata gaaagacgct atcatgtata tttcagcttt
     1081 ttgacaaaga taattgtgat gaagatgatt gtctaaaaac tataaaagaa aatatgaaaa
     1141 atgataagtc tggatatttt aattataact tagataatga aaaatttatg ttaaactatc
     1201 agccacttgg agttaatgat tggtatgttt tttctgttgt gccaaataaa atagtatcta
     1261 ttcagtcaga gaatatatct tatgatgtat atatttttgc attagaaata ctactagtta
     1321 tattgatttt tataatatat atagcatgta ttataaataa taacaccaaa attattataa
     1381 atgaaaaaga aaaatttaaa gtactcactg ataatatatt aggaggggtt aaaacctgtt
     1441 taaaagatga atatatgact atgacatatg taagtcaggg gtttttagag cttacaggtt
     1501 actcttgtga agatttaaat aaaatattta aaaatagatt taaagacatg atttataaag
     1561 atgatttaga caagtttaat tgtattattc taaatcaatt agaaaaacaa gataaaattg
     1621 aagttgaata tagaattgta agaaaagatg gaagtataat ttggattttg gagagaagta
     1681 agtctataaa tgataataaa ggaagtgagt gtattcaggg agttttaact gatataacag
     1741 aacttaagaa aatacaagaa gaattagatt caaaaagatt tgaaatacag gctatcaatg
     1801 atagtattgt tggtggaata gtgataactg agataaatga tgattttgat agtatatatt
     1861 taaatgaagg ttatttaaat atgattggat atacacgcaa acaactggaa gatgagctaa
     1921 ataataaatt aatcaaactt atttttaaag atgatagaac agaattaatt aatagtataa
     1981 acaaacaact caaattaagt gatacatttg catctgaaca aagagttgaa aaaagggatg
     2041 gcactattat atggatttta ttaaaaggaa gaaagatgat ttatgataat ggaaaatctg
     2101 tactttgttg tgttattgta gatattactt ctgctaaatt attagagcaa gaacttaaaa
     2161 agagtgaaga acgtcatgaa atcattctaa agcagacgca aaatattata atagaatggg
     2221 atatagaaaa agataaaatg aatatatcac ctaattggta taataagttt aaatatgacc
     2281 ctataactga aaacgcaaca aaaaatataa ttaaatcagt agatatatat catgatgata
     2341 tggataagtt tatttcatta attaaactta cttcacaagg tagtgagtat gaagagtgtg
     2401 aaattagact tagaactaga gatactattt acgcttggtt tagtataaag gcaacaactt
     2461 tatttgataa taatggtaaa ccttataaag tagtaggaag tttgactgat attgatgaat
     2521 caaaacgaga agctgaaaag ttgaaagaaa gggcagaaaa agaccctctt acagatctgt
     2581 ataataaaaa agcatcacaa tcacttatag aggagtacat aacattggat aaaaaaagaa
     2641 aaggtgcttt aatgattatt gatattgatg actttaaagg aataaatgat aatttaggac
     2701 atttatatgg ggatgctgta ttaagtgaaa ttgcaaatga cctaataaat ctatttagag
     2761 caaccgatat agtaggcaga attggtggag atgagtttat tgtatttata aaagatattt
     2821 ctgaaacaag agatattata aataaagcag aagaattggt aaaaacattt gaaaggtctt
     2881 ttttaggtaa aggacgaaat tataaaatat cattaagtgt aggaattgct aagtttccac
     2941 aagatggtat ggactatatg acactattta gaaatgcgga tatagctctt tactcagcca
     3001 aagggaaggg caagaattgt tatacattgt atgataaaaa atttgataaa gtccaatata
     3061 taactaatac aagaaatgca ggagatagtg ataatgtaga gatgtctttt aaagaacata
     3121 tcacaaatta tatatttgat atactatatg aatcaagtga tatagaagtg gcagtaaatt
     3181 taattctaag tattgtaggt aaacacttta atgtgagtcg catgtatata tttgaaaatt
     3241 cagatgataa tcgagtatgt aaaaatacat ttgaatggtg taacgaaggt gtaacctcgg
     3301 aaattaataa tcttaaaaat atttgttaca aggatttagg caactatctt ttaaatttta
     3361 atgcagatgg gttattttat tgtaatgata ttgaaaattt aggtagtgat gtgaaaaaag
     3421 tacttaagcc acaaggcata cgttcaatat tacaatgtaa aattttagag ggtaatgaat
     3481 ttagagggtt cataggattt gatgaatgtt catataatcg tatgtggact aatgatgaag
     3541 tgaacacttt gacatttata tctaaaacgc tcagtatgtt tttattaaaa atgcgtattc
     3601 agagtaagct agaagagtct ttaacaattc aaaaatctgt gatggataat atggatatat
     3661 gggcatatgt tgttgacagg aatacctatg aattactatt tatcaataaa aaaatgcttg
     3721 agtcagcacc aaacactcat gtaggtgatt tatgttataa agcgatatgg aataatcaag
     3781 ataaaccttg taaaaaatgc cctatgatat atttaaaaga tggtgatgat acttataaat
     3841 cattcatgca taataaaaag tttgaggtac aggcaaatat aactacaact actttgaaat
     3901 ggtttgatgg taaagatgca tgtttaatat ttggaagtga aataactagt attgaagctg
     3961 aagaaaatac ttaaaatact gtgttatata tatacttaaa atcgcaattt gaatttatta
     4021 gacttaaaat gtctatataa atcatttaag ttgtggtttt aagtattttt tatgatataa
     4081 ttaagcgtta agaacaaaat taaaagatac ttttgtataa aaaaatattt gttaaatata
     4141 tattgagcag gtgataatat ggaaaataag tttttaccaa taagcaaaca agatatgata
     4201 gatagaggat gggaagaatt agattttgtt ctagttacag gagatgctta tgtggaccat
     4261 catagttttg gaacagcaat aatatctaga gttttagaaa gtgctgggta taaagttgga
     4321 ataatagctc aaccagactg gagaactaca gatgatttta tgaagctagg aaaacctaga
     4381 cttgcatttc tagttaatgc aggaaatatg gattcaatgg tcaatcatta ttctgtaagt
     4441 aaaaagcata gagaaaagga tatgtattct ccaggtggga aaatgggtta tagaccagat
     4501 agagctacta tagtatattg caataaaata agagaagcat acagtgatgt tgcaatagta
     4561 ataggtggaa ttgaagcgag cttaagaaga tttgcacact atgattattg gagtgataag
     4621 gttagaaagt ctatgttaat agatagtgga gcagatttat tagtttatgg tatgagtgaa
     4681 aagcagattg tagaagtagc caatgcactt aatgatggat atgacccaaa atacattaga
     4741 catattgatg ggacatgcta tatttcagat acattggaag aaatatatga taaatatata
     4801 ttaattccat catataaaga aatttgtgaa gataaaatga agtatgtaga agcttttaaa
     4861 attcaatatg atgagcaaga cccatttaga ggtaatataa ttgttcaacc tcatggaagt
     4921 aagtatctgg ttcaaaataa gccagaaaaa ccacttagta gagaagaatt agatgaagta
     4981 tatggattac catatcagaa gacataccat cctgtatatg aaaaatttgg aggaatacca
     5041 gctatagaag aagtaaaatt tagtatagta agttcaagag gctgttttgg tagttgttca
     5101 ttttgtgcaa taacatttca tcaaggtaga gcagtgcaaa gtagaagtga aaaatcaatt
     5161 attgatgaag caataggaat aacaaattta gatgatttta aagggtatat acatgatgtt
     5221 ggaggtccaa cagctaattt tagaagacca gcatgtaaaa aacaaattac taaaggtgct
     5281 tgtaaaaata gacaatgctt atctccatca ccatgtaaaa atttagatgc agaccacagt
     5341 gaatacttac atttacttag agctgtaaga aaattaccta aaataaaaaa ggtgtttgtg
     5401 cgttctggaa ttagatacga ttatgtaatg gcagataaga acaataaatt tcttagagag
     5461 ttgatagagc atcatgtaag tggtcagtta aaggtagccc cagaacatat atcagaagaa
     5521 gttttaaagt atatgcaaaa accagcagga aaaacttatg ataaatttag acagaagttt
     5581 tttgctataa atgaagaatt aggtaaaaag caatatttga taccctattt aatgtctagt
     5641 caccctggtt ctaccttaaa tagcgctata gagttagcag agtatttaag agatactcac
     5701 taccaaccag agcaggttca ggatttttat ccaactccag ggactctatc tacaaccatg
     5761 ttttatacag gtattgaccc gcttacaatg aagccagttt atgttcctaa atcaaaacgt
     5821 gacaaggcaa tgcaaagggc tttattacaa tatagagcac ctagaaatta tgatttggta
     5881 tactctgcgt tagttgaagc aggaagagaa gacttaattg gttttggaca tagatgtctt
     5941 ataaagccaa aaaatgaaaa gccttatttt aatagaaata attctaaaaa aaatgtatct
     6001 aaaggaacaa ataaaaacaa aaaaactaat acaaataaca gaagtaataa aaatcaacaa
     6061 aaaagttcaa ctaaaaaaaa gaaaaaacat taaagtataa ataaaaatac ttaaaaaaga
     6121 aggattattc gataaattaa cagaaattag tataaataaa tataattatt ttaatgacgt
     6181 tacctttggg aggaagagat gaaaaagtat atttctataa tattattagt ggtgttgact
     6241 atggttgttt tggtaggatg ttcaccagga aaagataacc ctaaaaataa ggagctatca
     6301 gttgtcaaac aagtaaaagt agctgttcca gatggactac cagcagtggc aatagctaaa
     6361 cttgcaaatg aaaatccaga gattaaagaa ggatatgaaa cagtgtatag cattgaaaag
     6421 acacctgaag cgatttccac tagagtgatg aaaaaagatg cagatatagc tatagttcct
     6481 tcaaacatgg ctgcaatagc gtataataaa acttcatcat ataatattgt tggaactgta
     6541 ggaatgggtt cactttattt ggtgtcaaca gaaaatataa aagattactc tgatttagtt
     6601 ggtaaagaag ttggatgtac tggaaaagga cttactccag acataactat aaaaagtctt
     6661 ttgttacaaa aggatataaa ttattcagat ataaaattta attatgtaaa ttcagcaagt
     6721 gaactggtac cattattggc tactggtaaa gttaaaacag gtattgttcc agaacctgca
     6781 cttagtgctt tgatgtctaa aaatccagac ataaaaatta taaaaagttt aaatgactca
     6841 tggaaggaag taagtggttc aaaagatggt tatcctcaat ctactttaat tgtaaagtct
     6901 gaatttttaa gagataataa agattttgtt gattcttttg taggtcaatt atcaaatagt
     6961 atagattggg caaacaaaaa tcctgaagaa ttaggtgcat attctgaaaa aataaaaata
     7021 tctactgaat caaagataat aggaaaatct cttgaaagag ctaatttgaa atatattcca
     7081 gttaaggaca tgattaaaga ttataaaaat tactatgaga aattagctaa ttttgatgat
     7141 aagacattag gaggcaaagt accagatgaa gcaatatact ttgttgagaa ataaaaaaaa
     7201 taaagaacaa tttatagata aaatggaggt aacactttct tgtgttatcc tcttattttt
     7261 atggcaaatt attgctttaa aaattgataa tgatattttt ttaccaacag taggtcaagt
     7321 gtttaatagt ataaaagaga ttatgctaaa ttctagtttc tatatagata tactttactc
     7381 tatggggaga tgttttttta gttttttact ggctatgatt tttgctatta tactttcaat
     7441 agtgtcttat ttaaatagat tttttagaaa cttattaaag ccaatcaatg ctttgacacg
     7501 ttcaatacca actatgatat tggtagtact tgcacttatt tggtttgaaa aggatagtac
     7561 cccttttata gttggattta ctatagtttt tcctatattg tatgataacg ttttgggcgc
     7621 tatattgaat atagacaaga acttattaga aatggctaat gtgtataaaa taaggtttat
     7681 agataaggtg ttaaaaattt atttaccagc cataaaattt caaattatat ctatactagt
     7741 atcaacattt tctttaagtt tgaaagttgc tatagcagga gaggtgtata gtcaacctac
     7801 atatggtatt ggaactatga tacagactga aaaaattaac tttaacacat ctggaatatt
     7861 tgcatggata attattgttc ttttaatatc tgctatttta caaatagctc aaaagttttt
     7921 agcaaggaga gcttttttat ggaaaagata aagttgagta ttgaaaatat aaataaaaga
     7981 tatgatagtc gtattatatt tagagatttt aatattgatt tttatgtaaa tgaggtaaat
     8041 tgtatccttg gaaaatctgg ttgtggaaaa actacactct tgaacataat aagtgggatt
     8101 attaaaaatg acaccaacaa tctaaatatc aaagaaaatt taaatagagt aggaaataag
     8161 cttgaagcta gttacatatt tcaagatgat agacttatag actggttaac agtagaagaa
     8221 aatataaaga tagttgtaaa taaatactat aataaaactc aattaaataa aatttgtgat
     8281 gaatacttgg aattggttgg aattagtgat tataaaaaat tctatcctca aatgcttagt
     8341 ggaggtatta gacaaagagt aaatattgct agagctttta tatatccatc aaaaaacatt
     8401 ataatggatg aaccttttaa atctattgat gcaaagaata cacagcttat aatggataat
     8461 tttagaaata tattaagaaa agaaaagaga actgtgttat ttgtaactca taatattgag
     8521 gaagcattgt ttttagcaga taggatattt attttaggag actctccaat tagaataaaa
     8581 aaaatattaa aaaattctaa ggaattggaa aaaaatgaag ttttgaagtt gatatagagt
     8641 tataataata ctgtcgtttt aaaatatatt tggttaaaat ctataagact atattaaaat
     8701 agtacagaat attcaaaact gtgtataatg ttcgtatttt ataaaaagga agtgaatgta
     8761 tggttaagac taatgctatg agaatattag attcaaataa gattgattat aaagtaatgt
     8821 cttatgaagt gaaaagtgaa catgtggatg gtgttgaggt ggcacatgat ataggaagag
     8881 atgtaaatga agtgtataag acattagtaa ctcaaggagt tagtaagaat atatatgtat
     8941 atgtaatacc agtacatgaa aacttagact taaaaaaggc tgcaaaagta gctaaagaga
     9001 aaagtgtaga gatgattcat gtaaaagata taaataagct tacaggttat attagaggcg
     9061 gatgttctcc tataggtatg aaaaagtttt ataaaacttt tgtaaatgaa agtgcaaaga
     9121 atttagatac aattattgtg agtgcaggaa aaataggata tcaaatagaa ctatctccat
     9181 ttgatttaca aagacttata aaagtagaat ttgtagatgt aataaaaaaa taagaattac
     9241 atataaaata tagagcttat attctcaata ataggtgttg taatattaat aaaatgaata
     9301 agaattttaa aataaatatt cagttactta tgtgtaattg gtataaaaat attgagaaat
     9361 tttaaaaaca atggtaaagt gtaagtataa tcacgaatat tttttataaa gtaatggagg
     9421 gatgttcgct tgagaaaaat aaaatctgaa caaatcgtcg agcaggtaaa aaaactttgt
     9481 atagaagcga gcctatattt aggagaagat gttttaagtt gtataaaaga aaaagcgaaa
     9541 agcgaaaaga gtgaagttgg aaaaaatata ttgaatatat tagttgaaaa tgctgaaata
     9601 gctaaagaaa aaaatatacc aatatgccaa gatactggta tggctgtatt ttttgttgaa
     9661 ataggacaag aagttttaat tgaaggagac acacttactg atgctataaa tgagggagta
     9721 agacaagggt atgaagaagg ctacttaaga aaatctgttg taagtccaat taacagagta
     9781 aatacaaaag ataatacacc tgctgttatt cattatgata tggtaaaagg tgataaaatt
     9841 aaaatagaat ttgcagctaa gggatttgga agcgaaaata tgagtaagat gaaaatgtta
     9901 aagccatcag atggattgga aggaattaaa aaattcataa tagatacagt ttcagaagct
     9961 ggacctaacc catgccctcc tatggtaatt ggagttggta taggtgggac tgtggacaaa
    10021 tgtgctcaaa tagctaaaaa ggcacttttt agagaactag gtgaatttaa taaggacgaa
    10081 aatatagcaa aacttgaaag tgaactatta acagctatta ataaacttgg tataggacct
    10141 caaggcttag gtggtactac aacagctttg ggattaaaca tagagacttt tcctacacac
    10201 atagcaggtc tacctgttgt tgtaaatata aactgccatg catcaagaca taagaatgta
    10261 gtaatttagg aggaaaataa aatgataaaa ataactacac ctgtaaatga aattgatata
    10321 gcaaaattaa attgtggaga tacaattagt ttgagtggta tactttatac agcaagagat
    10381 gctgctcata aaagattgat agattgtata aataaaggtg aagaattacc atttgacgta
    10441 tatggtcaag gaatatatta tgtaggacct actcctacaa agccaggaga agttataggt
    10501 gctgcaggtc caacgacgag ttatagaatg gatgatttga ctatacctct tttagaaaga
    10561 ggtcttagat tgatgatagg caagggaaaa agaagtgatg aagttataga aggtatgcaa
    10621 aaatacggag cagtttatct tgctgcaata ggaggagctg gagcgtatat ttcaaattca
    10681 ataaaatcat gtgaaattat tgcatatgaa gatttaggag ctgaagctat aagaaagatt
    10741 gtagtagaag accttaagct tacagtagca atagatagct atggaaataa tatatatgag
    10801 caaggtcgag caatttatga atgtaaataa tgtttagaaa taaatatgaa tatcaaaatt
    10861 aaaaatagtt gtgtatattt taaggaggaa aattgatgtc taaggattat gcaaaattgg
    10921 cattagaaat gcatgaaatc aacaaaggga aagtaagtgt agaaagcaag gtagaaatta
    10981 aaactaagga tgatttatca acagcatata caccaggggt tgctgaacct tgtttaaaaa
    11041 tacatgaaaa tcaagatgat gtttatagat acacttctaa agggaatcta gtagcagtag
    11101 ttagtgatgg aagtgctgta cttggattag gtaacattgg tgctgaagca tctatacctg
    11161 ttatggaagg taaagctata ttatttaaac aatttgcaga tgtagacgct tttccaatat
    11221 gtttaaagac taatgatgta gatgagatag ttaaatctgt agagttgatg gagcctgtat
    11281 ttggaggtat aaatttggaa gacatatctt ctccaagatg ttttgagata gaagagagat
    11341 taaaaaaatc tttaagtata cctgtttttc atgatgacca acatgggaca gcaataatag
    11401 ttgcagccgc cataataaac tcaataaagt taattgaaaa taaaaaaata gaagatttag
    11461 aaatagttat aaacggagca ggagctgctg gtatagccat agcaaagata ctacttaata
    11521 tgaatgttaa aaatataatc ttatgtgata gaagtggagc attggaagct agtatagaaa
    11581 atctaaacta tgttcaaaaa gaaatgttaa aagtaacaaa tataagaaat gaaaaaggac
    11641 ctttaaaaga tattataaaa ggaaaagatg tatttattgg tgtttcaggt ccaggagctg
    11701 ttacaaagga aatggttgaa agtatgtctg agaagccaat aatattggca atggcaaatc
    11761 ctactccaga gataatgcca gaagaagcaa aattaggtgg agcatttata atgggaacag
    11821 gtcgttcaga cttccctaat caagtcaata atgtactggc ttttcctgga atatttagag
    11881 gtgcacttga tgttaaggct aaagagataa atgaagaaat gaagatagct gctgcatatg
    11941 caattgctaa tacaataagt gatgaagaaa tatgcccaga gtatatatta ccagatgtat
    12001 ttaataagaa tgtagtaaaa aatgttgcta aggcagttaa agaagcagcg ataaaaacta
    12061 aagtaaatag aatataaatt tttatatgtt catatatcct tatattatcg ggtatatggg
    12121 catttttatt ataggtttat actcatatat ccttactttt atatacatat ctttatgatt
    12181 aaaggtgtta gaaaatgtag taaattttat actatcagac tctatcttta ttactttagt
    12241 agatacatgg ttgtaaaggt ttatagaaaa aaatatagga aggcagattt ttattgttgc
    12301 gtatatatga gaatgatata atatgaatat tataaattaa taaatttaaa tttatggagg
    12361 tacaagagat gatggaatat aaagttaatg accaagaact taatgcttca atgtttatcc
    12421 cattcgtcaa caggatatgg ccaggagatt atgatatgga taaaacacag actgcattat
    12481 caaaaacttt gaatataact gcttatgatg ataaagtgct tgtaggatgt ctgcgagttc
    12541 tttcagatgg ttattatttt ggaacaatta cagaattgct tgttcttcca gagtatcaga
    12601 aacaaggcat tggtagtaag cttttccagc ttgctaaaga gaacacccca actatgctat
    12661 attttggtgc acagccagga gtagaagcgt tctatgaaaa aaatgggtgc caaagaagtc
    12721 tgcaatccta cataataaaa aagaaagata gttattaaag ttccagcttt caataatctt
    12781 aaaagtacaa tattttagtg gaatcatttc attttttatt actaatatga cataaacatg
    12841 tcatattagt attgatatac ttttaatatg gttaagagcc aaagaaaaat ataaggagtg
    12901 atttaaaatg gaagaaacta aattttgcca atgctgtgca atgccaatgg gacaaactga
    12961 tgaattatat gggacaaata aagatggaag taaaagtact gattattgta gttattgttt
    13021 taaagatggt gagtttacag ctgatataag tatggaagag atgatagaag tttgtatccc
    13081 tcatatgctt caaagtaata aggatatgac tgaagatgaa gcaagaacaa tgatgaacaa
    13141 tttctttcca acattaaaac gctggaaata attttatgca aaattagtta tatgcaaaac
    13201 atgaaatatg gtgttgagaa taaggggtgg tgtgctttat agcataccac ctgttttgtt
    13261 acttggagtt cagttatttt cctaaatatt ttaaatatac tattttagat aagttccctc
    13321 ttcatcttga taaattaatc cttttttcat aagcactcca agagctcgtt taaaattttt
    13381 cttacttgaa ttaaataata tagaaatatc tttagggtca gatttatcat taaatctcat
    13441 atatccatca gaaccttcta aataacttaa aatcttattt tctaaagtat caagttcatc
    13501 ctttctattt cctctaggag aaagaccaag ttttccatct tcatatattt ttatgacatg
    13561 taaattttca agaacatcac cagtttttag ttcagtaaag tactcattat gaagtattac
    13621 accagcatat ttattgtcta cacatatcat tgcagaatta ttagtttgga atccatatac
    13681 aactccagtt acaatatctc ctacattgta agtatggtct gttaataagt atgaatctat
    13741 gtctgtagta gcagcaattc ttttgctttt gtctaaatat atataaaata aatatttttc
    13801 gtctctaaat agtggataag tcttagcttt aaaaggaact aatatgtctt taggaagtcc
    13861 catatctata aaagtaccaa tgtctgtatt atcaactact tttaaatgtg ctacatcccc
    13921 aaccttagct aaaggcggta ttaaagttgc agcagttctg tcatcagaat ctttaaatat
    13981 gaaagcattt acttcatcac ctatgtttat ttcatttttt ccgattaatc tattatgaag
    14041 aagtatatcg tcttttgtgt tattagtttg tccatccaaa aaatatccaa attcagtttt
    14101 tctttttact gtaagtttat taaaatcacc tatgtttatc aaattaaaat catcctctca
    14161 ttttatattt ataatttagc cataatatac ttatgtagta gtttgtcaca gtatccatat
    14221 tagtattagt gaaatttaca ataaataata tagttattta tgtatttttc aattctctag
    14281 cttcttatat agtataaact ccaatttctt ttttgtcttt aggttttatt aaaattttta
    14341 caaaataagt taaaaagtat tgaaataaaa tttcttttaa agtataattg gtatatacca
    14401 aaattggtat ataccatatc gaaaagtggt gactatatga aaatagtaaa taaatctagt
    14461 aatataccat tgcatactca attaagttca ataataagag agatgataga aactggtgag
    14521 cttaaagagg gcgatgcaat aatgccagaa agagaacttt gcaacataca aaatgtaagt
    14581 cgtatgacag taaataaaac cattgttgga ttagttacag aaggacttct ttatagggta
    14641 caaggtaaag ggacattcgt tgcaaagcaa aaaaagaagt atcagttttc aaatgtaaaa
    14701 ggttttactg atgttatgaa agaaaaggga gtaaacataa aaacggatat attatccttt
    14761 gagatggaac taccagatga tttagttaaa agaaagcttg gaataagtga taatactaca
    14821 aatatatata agattgtcag acttagatat actgatggag agccatttgg attagaaata
    14881 gtatatctat cagaagaaat gtgtaagggt ctaacaaaag ggatattgga taattcttca
    14941 ttatataggg ttttaaatga aaaatatggc tataagatac aaaaagctga acaggtaatg
    15001 gagccagtaa tactttcaga tgaagaaagt aagcttcttg aaacagatga aggtgcttta
    15061 gctcttaaac ttcatagaaa ttcctataat agagaaggta gccctataga atataccata
    15121 tcaatattta gaacagataa atatcaatat gaaatagtat tgagtgagta ggtgaataat
    15181 atgaaatgtt taattaatgg aaagataata ttaaaaaatc aaatcttaga aaataaagta
    15241 ttagtatttg atgaaaaaat aattgatata gctgatagtg taccaaaaga ttgtgaagtt
    15301 atagatgctg atggaaaata tatatctcca gggcttattg atattcatat acatggaaac
    15361 atgggaaaag atacaatgga ttcgactgat gaatctattg aaacaatttc aaagtcaatt
    15421 atgagacatg gagtaacatc ttttcttcca actacaatga ctatggataa agaacatgtt
    15481 tatgatgcac ttgaagttat taaaaaagcc caaaatagaa agcttgaagg ggcacaagta
    15541 ttaggagcac atttagaagg accttttata aatgagaatt ataagggagc acaaaatgaa
    15601 aaatttataa taaattcaaa atacgaattt ataaaagaat ataaagatgt tataaaagtt
    15661 ataacttatg ctccagaaaa agatattgat tttgatttta ctagagaaat aaaaagatgt
    15721 acagatatag ttttatctat tggtcatagt aatgcaaatt atgaccaagc taaagaagct
    15781 ataaatttag gagtaactaa tgtaactcac atgttcaatg caatgactgg tttaaaccat
    15841 agagacccag gcgttgttgg tgcagcactt acaactaatg tgtacagtga gttaatagca
    15901 gatacaatcc atataaataa agatttattt caatttatac taaataataa aggaaaagaa
    15961 agactgattt taataacaga ttctatagaa gctggtggtc ttgaagatgg caattattct
    16021 cttggaggtc agaaagtcat agttaagggt aatgaagcta gacttgaaaa tggagcatta
    16081 gctggaagtg tgttgtcttt aaataaaatg gtatttaact tcttagataa tacaaacctt
    16141 aaagtgaatg aagcaattaa tttagcttct ttaaatccag caacatcttt gggaataaat
    16201 gacaaaaaag gaagcttaga aataggtaag gatgcagata tagctgtatt tgatgagaat
    16261 ttagattgta aaatgacact ttgcttgggg gacgtagtat ataaaaatat atagtataca
    16321 aaacaaacct taaagacagt aaaaacaata taaaaaataa taaattaaac ttacattagt
    16381 aaaagtagta gttaaaattt aggaggaata agatgagagt attagtgtgt aaagattatg
    16441 atggaatgag taaaaaagcc gctgaaatga tagcagctca aatagtttta aaaccaaatt
    16501 caatactggg actagctaca ggaagtacac cagtaggtat gtatagagat ttagtaaaaa
    16561 aatacaatga taatatagta gatttttcag atgtgatgag ctttaattta gatgaatatt
    16621 ataagttacc aatatctaat gaccaaagct atgattattt tatgaaagaa aatctattta
    16681 atcatgtaaa cataaagcca gaaaatactc atctaccaaa tggaatggct gatgatattg
    16741 aaaaagagtg tatgaactat gaagcttcta tagatgcagc tggtggtata gatgtacaag
    16801 tacttggaat aggtcgtaat gctcatatag gttttaatga acctgatact aaatttgcaa
    16861 agagaactca tgtagttgaa cttacagagt ctacaataga agctaatgca agattcttta
    16921 aatcaagaga agatgttcct aaaaaagctg taagtatggg aataggttca atattgaagt
    16981 ctaagaaaat attgctttta gcttcaggag aagaaaaggc tgatgcagta tataatactg
    17041 tttatggaga tataactcca gaagttcctg gttctatact tcaacttcat aaagacacta
    17101 tagttatagt agatgaagca gcagcaagta aattgaatcc aaaagattat aaattagtat
    17161 aagaatagaa tgtagtttaa gtaaaaaata gctatcattg agtaatcaaa tttatttaat
    17221 gatagctatt tttatttaaa ttttttactt ctttttattt aaattagtta ttattgttta
    17281 aattacttgc ttattacttt ttacgaaaac tcgttatcaa gaagtatcca aataagatgt
    17341 taaaaattat tatgtttagt gggattctat caagcataat aaaattacct ataattggga
    17401 tattaccaac tggaaacata aagaagaata caggcatcag tgatagtaaa aaagatggaa
    17461 aacaaagaag aagtaatctt ggaatatcaa tataccaact accatccttt ttgtattctc
    17521 taatcaataa ttcagttcct agtaataaac caataccacc agaggaaatt aaattaataa
    17581 aaatgtatct tttaaagtct tgcatataac cagtgtaaaa ttgagtatag aaatcataat
    17641 ttaatttaac agctaaaaaa tacataagca atagaatgat taaaaataaa agatatctaa
    17701 gagctttttt cattttaaat tctcccttca gaaatacttt attcagtttt attttgttgg
    17761 aatctgattt tatcatttag tttaagaaat ccataatatt agtgaagaat gaaataagta
    17821 ttatatatga ataaatattt ttagaattaa g
//
LOCUS       DAYAOX010000071        17834 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.071, whole genome shotgun sequence.
ACCESSION   DAYAOX010000071 DAYAOX010000000
VERSION     DAYAOX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.071"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            422..1255
                     /locus_tag="ACGI57_001827"
     CDS             422..1255
                     /locus_tag="ACGI57_001827"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9716906.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1324..2280
                     /locus_tag="ACGI57_001828"
     CDS             1324..2280
                     /locus_tag="ACGI57_001828"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9716907.1"
                     /translation="MNKKLYIGMVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVL
                     SATNNYINGQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVD
                     SKYQELEDKMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDM
                     EAYYTSHKKDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESF
                     ESLAKKYSDDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVK
                     VTDKRERQKSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2332..2814
                     /locus_tag="ACGI57_001829"
     CDS             2332..2814
                     /locus_tag="ACGI57_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9716908.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2826..3695
                     /locus_tag="ACGI57_001830"
     CDS             2826..3695
                     /locus_tag="ACGI57_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFG9716909.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3738..4493
                     /locus_tag="ACGI57_001831"
     CDS             3738..4493
                     /locus_tag="ACGI57_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFG9716910.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLASLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4535..5356
                     /locus_tag="ACGI57_001832"
     CDS             4535..5356
                     /locus_tag="ACGI57_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HFG9716911.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5361..6122
                     /locus_tag="ACGI57_001833"
     CDS             5361..6122
                     /locus_tag="ACGI57_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFG9716912.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6283..6540
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6653..6892
                     /locus_tag="ACGI57_001834"
     CDS             6653..6892
                     /locus_tag="ACGI57_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFG9716913.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7145..8191
                     /gene="ilvC"
                     /locus_tag="ACGI57_001835"
     CDS             7145..8191
                     /gene="ilvC"
                     /locus_tag="ACGI57_001835"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFG9716914.1"
                     /translation="MSNKNKYHKNKKKYWEEMKMARMYYEKDVDLEVLKNKKVAVLGY
                     GSQGHAHAQNLRDNGVHVVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPD
                     EKQKKVYEESVKDNLKEGQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVF
                     TKGSGVPALFAVYQDYTKKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVL
                     CGGISELIKLGYKTLVDAGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEY
                     GDYVSGKRVITDVAKQGMQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIA
                     EVGRNLRSMMSFLK"
     gene            8244..9932
                     /gene="ilvB"
                     /locus_tag="ACGI57_001836"
     CDS             8244..9932
                     /gene="ilvB"
                     /locus_tag="ACGI57_001836"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFG9716915.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            10143..10820
                     /locus_tag="ACGI57_001837"
     CDS             10143..10820
                     /locus_tag="ACGI57_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFG9716916.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            10989..13025
                     /locus_tag="ACGI57_001838"
     CDS             10989..13025
                     /locus_tag="ACGI57_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HFG9716917.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPAGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(13216..13782)
                     /locus_tag="ACGI57_001839"
     CDS             complement(13216..13782)
                     /locus_tag="ACGI57_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9716918.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            14070..15005
                     /locus_tag="ACGI57_001840"
     CDS             14070..15005
                     /locus_tag="ACGI57_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HFG9716919.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            15470..16684
                     /locus_tag="ACGI57_001841"
     CDS             15470..16684
                     /locus_tag="ACGI57_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9716920.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(17104..17625)
                     /locus_tag="ACGI57_001842"
     CDS             complement(17104..17625)
                     /locus_tag="ACGI57_001842"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9716921.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHKQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEIRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHKKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
BASE COUNT         7308 a         1758 c         3042 g         5726 t
ORIGIN      
        1 aaaatattac ctgattaata tagtgattta taaatgagat tagaaaattt tatttgataa
       61 caaaaattag ataatatgaa tatataatgt atgtattaaa aatgtggcaa atagttgttt
      121 tgctatttat tatttgttta atatttctaa tttataaaaa aaaaattata aattttaaaa
      181 attataaatg acattttaac tagaattaat tgtaaaaaac actatataat actcaatttt
      241 caacattcta tacaattata cagaaattat acagcataat aatcaccgaa ataacaaatt
      301 ttaaaaatta agcaaaactt tttgtcgaag ttcaacgtct atatatatgt agtgtaaatt
      361 tttgggataa aatactacaa aaatataaat gtaaaaatat agtaatattg gaggtcgaac
      421 gatgagaaat aaaaagaaaa aaataaatag aaagaagttg tatttgcttt taagtgctat
      481 tgtagtgtgt ctggctttta tagtagttgg tgttcgcgtc agctctttaa atatgaagga
      541 aaatgaaatt agtagaaatg caaaactgtc aagttctact attacagata tagtatctaa
      601 tacatcaata gaagttagta aagggccaag taaaagttct ggaaaaatag catacataac
      661 tatagatgat ggtccatcta aatttactga ccagatgata aaaactttaa ataaatacaa
      721 tgttaaagca acttttttta tgatagatgg gaatatgaag gaatatccac agcaagttaa
      781 aaatataatt aaaaatggga acacagcagg ttttcatagt gtatcacatg atattcacaa
      841 gctttatgtt actagtacat cagcaaaaga ggaatttgat acaaatgatc aaacttttta
      901 taagataact ggtaaacatt caaaagtaat aagaatacct tatggaagca aaccatatac
      961 accacaagcc tcttatcagg ctttagttga tgctggatat aaaatttggg actgggactt
     1021 agatacagaa gactggagat ctaattcttc tcaaattgta caaaatgtaa aaaatcatat
     1081 taagaataga aaaggtgaag ataaagacca acttgtagtt ttaatgcatg agaaaaaaca
     1141 gagtgcagaa gctttagatt cagttttaaa attcttatca gatgaaggtt atgaatttgc
     1201 gccagtagat caaaaccaaa taccaaaaaa ttattggtta cgtaatttag agtagttaaa
     1261 atataagaat attgattgat atttttagaa attgagaata aaaatgagtg aggtgtaggt
     1321 atattgaata aaaaacttta tataggaatg gtcggaatat tgtctttgat gatggttggt
     1381 tgtaataaaa gtttagcaaa agtaaatgat gtggaaatca caaaagaaca gtacaaaaaa
     1441 acaaaagcag ttttatctgc tactaataat tatataaatg ggcagtcttt agatgaatta
     1501 gaaaaaactt tggataaaaa aggtagaaat aaattagaaa atgttataat atcgtttatg
     1561 gtggataatg aacttttata tcaagaggct aaggataaag gactaactcc aagtaaaagt
     1621 gaagtagact caaaatatca agaactagaa gataagatga atttgaatac aagctataag
     1681 gaaaaaatgg acaaagcagg tgttgataaa gattatttaa aacaagaaat atctagagat
     1741 ttagcaatag ataaaaataa aaaggccttt gaagatagaa taaacataag tgataatgat
     1801 atggaagcct actatacaag tcataaaaaa gattttaatg tagaagaagt aagtgcttct
     1861 cagatactta tatcgacttt agataaaaat aaaaaagaag taagcaaaga taaaaaagaa
     1921 gctttgaaga agaaggcaga taatatattg actaaaatca aaaatggaga aagttttgaa
     1981 agtttggcta aaaaatattc ggatgataaa gcaactggaa aaaatggagg acagctagga
     2041 tattttacaa aggatgataa aaatgctgag tttacaaagg aagtgttcaa attaaagaaa
     2101 aatgaagttt caaatgtgtt tgaaactagt tatggatatc atatagtcaa agttactgat
     2161 aaaagagaaa gacaaaaaag ttttaatgag tgtcagagtt taataagaga atctatttta
     2221 aatgaaaagt atattgagca tataaaaaaa ctgaatgaag atgctaaaat agacagatag
     2281 aatttctaac agctcaatcc tagcctgtaa tcggagggag attttacagg aatgtttaag
     2341 agtttaaaga gaacgaaagt tgagaaatac attatcgata ataaagactc tttttataga
     2401 atagcatata gttatacgaa gaatgaagaa gatgccttag atgtggttca agaagccatg
     2461 tataaggcac tatactcagt ggaaaatatt aaagaagtaa attatataaa gacatggttt
     2521 tataaaattt tagttagaac gagtatagat tttattagaa aaaaccgtaa atataataat
     2581 atgacagata ttgaccttat agatgagact ggagagtatg ataaatatac tgatttggat
     2641 ttaagaagag ctttggaaga attacctatt gaatacaaat ctattataat attaagattt
     2701 tttgaagatt taaagattga agaagtagca ataatattgg atgaaaatgt gaatactgtt
     2761 aaaactaggt tatatactgc tttaaaaaaa ctaaaattaa aaattgaaga gtagggataa
     2821 aaagaatgac aagcaaagaa aggttaaata agttaaaaga tgagtatcat aaaattccaa
     2881 tacccaagaa attagatact ataataaatc atgaaaaaat agaaaacata tatcgagaga
     2941 aaaataaaaa agtaaataga ttaagattta aagttgcaat agcctttgca tgtatattta
     3001 cagtattagt aaatataagt cctgtatttg cagacaattt ttctaagata cctgtaattg
     3061 gagcaatagt agaagtaata actataaaaa attatagttt aaaaagtgaa aattatgaag
     3121 ctgagattga tattcctaaa atacgaggtt taaaagataa aaacttagaa caaagattaa
     3181 acagtagttt tatggaagat ggaaaaaggc tttatcatca attccaagag agaatggaga
     3241 agatacaatc atcaaagaat aaaggatata aatctttaag tttgagttat agtgtaaaaa
     3301 ataattctaa aaaatttcta tcaatagaga tgacaaaaaa tgagatagag gcttcatcat
     3361 atgtgagtaa ggtacattat actattgata aaaaaagaca aatagttttg actttgccaa
     3421 tgctttttaa agatgataag tatataaaag ttataagtga taatataaaa gaacaaatga
     3481 gagaacaaat gaaaaaggat agtacaaaat cctattttat agatcaaaaa aaagacttgc
     3541 ctgttgaaga ttttaaaaca attaataagt atcaggattt ttattttaat aaaaatgaag
     3601 accttgtaat ttgttttgat gaatatgaag tagcacctgg ttatatgggc gcagttgagt
     3661 ttgtaatacc atataaagta ataaaagatt tgtaaattga atagtagttt atatataaat
     3721 cttttaagga ggacaatatg actacatata aaaaacttaa aaaatctata tttaaaattg
     3781 ctatattttc aatatgctta ttagcttcat tagtagatac aaaccctata tttgcaggta
     3841 gttcctttaa tgaaacttca aatagtgata cgataaaggt gttaaatgct aagagtatca
     3901 actttaaaca agacaattat gaagtgaatg tgagtattcc taaaataaaa ggtttaaaag
     3961 ataaaacttt agagaataaa ttaaacaatg agtttttgca agatgggaaa aaaatttaca
     4021 gtgaatttca aaataaaatg aaaaaggaag ataataaggg tcatagatac ctaaatgtaa
     4081 tccataatgt caaaaataat tctaaggact ttttatcaat agaaatgaca aaagaagaga
     4141 tagaagcttc ctcatctata actaagaagc attacactgt tgataaaaat agaggaatac
     4201 tattaaattt accaatattt tttaaagata gtagatatat tagagctata agtgataata
     4261 taaaagagca gatgagaaat caaattaaga aagatgatac aaaatcttat tttatagacc
     4321 aagataaaga ctcgccagtg aaagattttg aatctataaa taagtatcaa gatttttatt
     4381 ttaataaaaa tggaaatctt gtgattagtt ttgatgaata tgaagtagct cctggttata
     4441 tgggagcagt agaatttgtg ataccaaata aagtaataaa aagactaaga tagatatata
     4501 gaagattttt agatagactg gtaaggagaa ttatatgaat atatatgaac aagcaataag
     4561 attaatagaa aataatgagg atttcgcatt tgctacaata acgtctcatt caggttcaac
     4621 accacgtgaa actggagcaa tgatgattgt gaaaaatgat tctactatat ttggctcagt
     4681 aggaggaggt agtgtagaag cagaatgtat aaaacatgca attaatgtta taaaaaacag
     4741 agaatcaatg ttgtataaat ttactctaaa caaatctgat gttgcaaaac taggaatgat
     4801 atgtggaggt actggagaaa tacaaataga ttttatagat agtaaattaa aaagtaatat
     4861 agaaaaattt aataagagac taaaggaaaa tacaagtaaa gcatatatat ttggtgcagg
     4921 tcatatttca agagatgttg ctgtgatact atctctttta gaatttagaa cagtagtaat
     4981 agatgataga gaagaatttg ctaatcatga aaggtttcca gaatcagaag tagtagtttt
     5041 agattctttt gaaaacatac cagattttcc aactgacgaa aatagttata taattatttt
     5101 aacaagaggt catctatatg attcaagtgc tttagaatgg gctcttaaaa gagaggctgg
     5161 ttatataggt atgattggaa gtagaacaaa aattggattg acttacgaaa aactcatgaa
     5221 aaaaggtttt aagaaggaag aattatcaaa agtacatgca ccaataggca taaaattgaa
     5281 tgctcaaaca ccagctgaaa tagctgtatg tatagctgct gaacttataa attgtagagc
     5341 taataaggaa aaataaacta atgaacacag ggagtaaaga tattgatact ttaaaggtta
     5401 attttagagg gattgaaata aaagcagttt tagtatataa aaatagaaaa aatatttcta
     5461 taaaaataga tccatttggc aaaataactg taatgagtcc tcctaaaata tccaaaaaaa
     5521 taataaaaga tataattatt gaaaaaggag actggatact taaaaagtca gaaaaatgta
     5581 aaggaagaga ggaagtatat aaacaaagaa tgtttataac tggcgaaaaa tttctttatt
     5641 taggtgaaga gtaccattta gttataaaag aactgttgag agattctgtc aaaaaaagta
     5701 actgtagaat aactattaac gaatatcaaa tagttgttca gacaaatgat acaagtacag
     5761 attttattaa aaaatcttta aaatcttggt ataagatgga aagtgaaaga atagtattag
     5821 agagaataga ttttcttaaa ttaaattgtg aaataatgaa gcaattagtt ccagcgagtg
     5881 ttaaggtaaa agaacaaaat aagagatggg gaagttgtac agctcaaaaa aatatttata
     5941 taaactcaaa aatatctatg gcaagattag atgtaattga ttatgtaata atacatgagt
     6001 ttagccattt agtacatatg aatcactcta aaaaattcta tgatttagta aaatccatta
     6061 tgcccgatta taaagataaa gaaaattggc taaaaataaa tggatataaa ataataatct
     6121 gaaaacgatt attccaaaat ataggcgtaa agtataaaag aagtattatt gtgaaaaaaa
     6181 taaataatct gaaaatttta aaaaaagtca ttgacattgt aaatgatagg gaatataatc
     6241 tatattaaca acaaaacaga attaaaatat aaaattaaaa aattaaaagc aatgacagag
     6301 acaacaatac taatagtgtt atccacagag aaccaatgtt gctgagaatt ggtgttaaca
     6361 gtagtataat gatcactctg gagcttttgt actgaatatt tttagtaagt acaaacgtat
     6421 gtcagcgtta catgactaag gttaaggtaa ttaattaccc gacctgaaaa aagaggggta
     6481 gatgtatttt ttatatctac aaataaggtg gtaccgcgga atataacttt cgtccttata
     6541 agtaatattt atattgctta taaagatgaa agttttttta ttttaagcga tataaataaa
     6601 tttaatccat ataaaattaa taaaaaaata ataaaaataa ggagggattt ttatggaaaa
     6661 tactgtatat gctaaattaa accaaggaat agattctttt ttaagagtgg ctatgactct
     6721 aagaagaaga gaggttgata tacaatcaat aagtatgact gtagataata tgaataattc
     6781 aggtataaaa ttaatagtaa atgaagaaaa gacatcctta gattctgtct taaaccatat
     6841 gaaaaaacta catgatataa gagagataac tgttgaaaga atgagacatt aattaaatat
     6901 attttataaa gctaaagata aatatgctta ttttaaaata aaaaactaaa gataaatatt
     6961 aaataagaaa aatcttagat taaaatataa gtgtcattga atttattagt ttatcacagt
     7021 taataaattt attagtttgt tgtagctaat aaaaactgaa aattctaaaa ttataattgt
     7081 aagattatat aaatttagaa tttaaatctg ttgaataata ttaaaacaat gataatgagt
     7141 atgaatttct aataaaaata aatatcataa aaataagaaa aagtattggg aggaaatgaa
     7201 aatggcaaga atgtattatg aaaaagatgt ggatttagaa gttttaaaaa ataaaaaggt
     7261 tgcagtttta ggttatggaa gtcaaggaca tgcacatgca caaaatctta gagataacgg
     7321 tgtacatgta gtgataggtc tttatgacgg aagtaaatca gcacaaaaag caaaagaaga
     7381 tggatttgaa gttaagagtg tagcagaagc tacaaaagaa agtgatttga caatgatgtt
     7441 aatgcctgat gaaaaacaaa agaaagttta tgaagagagt gtaaaggata acttaaaaga
     7501 aggccagaca ttagcttttg ctcatggatt caatatacat tataatcaag tacaaccacc
     7561 agaatttgtt gatgttgtaa tggtagctcc taaaggacct ggtcacttag ttagaaatgt
     7621 atttacaaag ggaagtggag ttcctgcact atttgcagta tatcaagact acactaaaaa
     7681 agctacagag acagttttag catatgcaaa agggatagga gcaacaagag caggtgtttt
     7741 agaaactact tttaaagaag aaacagaaac agatttattt ggagaacaat cagtactttg
     7801 tggtggaata agtgaactta taaaattggg atataaaacg cttgtagatg caggttatca
     7861 aaaagaagta gcatattttg aatgtttaca tgaaatgaaa ctaatagtcg accttatata
     7921 tgaaggtgga tttgaaagaa tgagatatag tataagtgac acagcagaat atggagatta
     7981 tgtatctggt aaaagggtaa taactgatgt tgcaaaacaa ggtatgcaaa atgttttaga
     8041 ggatatacaa aatggaaaat ttgcaaaagc gtggattaaa gaaaatgaag aaggacgtga
     8101 aaacttccta aaaactagag aagaagaata taatacagaa attgccgaag ttggtagaaa
     8161 tctaagaagt atgatgtcat tcttaaaata aatatatatt aaatggatta gtttgaggga
     8221 aagagtggtg tatagtagtg cgtatgaatg gagcaaaggt aattttagaa tgtttaaaga
     8281 aagagggaat tgacacaata tttggatatc ctggtggagc cgtgatacct ctttatgatg
     8341 ctctatacga ttattcagat gattttaaac atataaggac atcccatgaa caaggtttag
     8401 ttcatgctgc agatgggtac gcaagaagta ctaatacagt aggagtttgt tttacaacat
     8461 ctggtccagg agcaacaaat gccataactg gaatagcaac agcttttatg gattcttcac
     8521 ctatggttgt aatttcaggg caggtgccaa ctagtttatt aggaaaagat tcttttcaag
     8581 aaattgacat aactggagca acattatcta tgacaaaaca taattattta gttagaaaca
     8641 ctaaagaatt agttcctaca ataaaagaag cttttagagt tgcaaattca ggtagaaaag
     8701 gacctgtact tgtagatgtt ccaaaagatt tatttttagc agaaatggat tttagtggtg
     8761 aggactatga tttatgtcaa atagatgatt atatggatta taagagtgat tttgatctag
     8821 atgatgaaac taatattaag cttttaaatg aagcaataga tatcataaaa gaatctaaaa
     8881 agcctataat atatgcaggt ggaggtgtta aatcttcaga cagtgaggaa attcttgaaa
     8941 aatttgcaac taagatagat acacccgtac taaatacact tatggggctt ggaaatatag
     9001 atagaaagaa tgaattatct cttggaatgg ttggaatgca tggaagtaga gaaagtaatt
     9061 tagcactttc taattcagat ttagtaatag cgataggtgc gagatttagt gatagagtga
     9121 taagtaaaag ttctgaattt gctaaaaatg caaaaataat acacatagac atagacccat
     9181 ctgaaataag taaaaatata gaatctaatg tatctttggt tggagatgta aagctagtcc
     9241 ttagtttatt aattgaaagg gttgaaagta agaataatag caattggaaa gaagagataa
     9301 agagatttag aaagagtgag ggtgtacaaa catatgaatt tcatccacaa aatatactta
     9361 aaaaaataaa tgaaaaatat gaaacattaa agaaacccac tgtggtagta actgatgtcg
     9421 gtcaacatca aatgtgggct gctaagtact ggaattttaa aggtaataaa agttttataa
     9481 catcagcagg gttaggaact atgggatttg gtttaggtgc agctattgga actaaagttg
     9541 gtaatgtaga taaaaatgtg gttttagtca ctggtgatgg aagttttaga atgaactgta
     9601 atgaactagc tacagttgca aattataatg tacctatgct tattttacta ctcaataata
     9661 gaacattagg aatggtaagg caatggcaaa agttattttc caatcaaaga tactctcaaa
     9721 ctgacattaa tgaaaatgtg gattatgtta aactcgtaaa tgcatataac attgatggat
     9781 ataaggtatc cagtatggaa gaattaggaa aggcgctaga tatgatagat tttaataaac
     9841 ctgtattttt gcaatgtgat atagataagg attatgatgt ttatcctatt gtagcaccta
     9901 atgatgcatt ggaaaatttg atatgtaact aaaacttata ctgaatattg ataaaatttt
     9961 ttattaaatt tttattagta ttcagttttt gttgtttaga gatagattgg cattttttat
    10021 ttttgcaaat aaatcaatga ctatataatt aagaacttat taaattttaa ttatacaatt
    10081 tagaatagta ttacaactta agtaaataat aattttgaat aataatttac ggaggtattc
    10141 atatgtttaa acacgataaa gcattattaa aagaagtaaa agtagaaaga ccaaatcctc
    10201 aatatgctgt tttaatgcaa gaacagttgg gtggtgcaaa tggagagtta aaagcagcta
    10261 tgcaatactt gtcccaaagt tttagaataa aagaccctca aataaaagat ttatttcttg
    10321 atatagcagc agaagagctt agccatatgg aaatggtagc acaaactata aatttattaa
    10381 atggacatga tgttgattat aatgcagtta atacaggcga aatagaaaca catgtattaa
    10441 ctggtctatc accagtctta ataaactcat caggagctcc ttggactgct aattacgtta
    10501 cagttacagg ggatttagta gctgacttac tttcaaatat agcttctgaa caaagagcta
    10561 aggtagttta tgagtacttg tatagacaaa tagatgataa atatgtaaaa gaaacaatag
    10621 atttcttact taatagagaa gaagcacata atgctctatt tagagatgct ttaaataaag
    10681 ttaaagatac aggttcaaat agagattttg gagttacaga agattctaaa ttatatttcg
    10741 atttgtctac tccagggcca aatcacgata ctaagataga tattaatcct ccttcttttg
    10801 aaaaaccttt aaaaaaataa tttgatattt tttattttgt tttagtagtt ataaaaatta
    10861 tttactagaa gaattaatta tatgaaaaat taaatatgta tagttaaaaa tgggtataca
    10921 cttattataa gtgtatatct atttgtttat tgcaaaacat ataataaaat aacaaatgag
    10981 gtggtatttt ggacagtaat agaaaaccta ataatctaat aaatgaaaaa tctccatatc
    11041 ttttacagca tgcttataat cctataaact ggtatagttg gaatgatgag gcttttaaaa
    11101 aagcaaaaga agaggataaa ccaatatttt taagtgtagg atattcaaca tgtcattggt
    11161 gccatgttat ggaaaaagaa tcttttgaag atgaagaagt tgctgaaata atgaatagaa
    11221 actttgtggc aataaaagta gataaagagg aaagacctga tgtagatagt gtgtatatga
    11281 cagtttgcca agctatgaca ggtagtggtg gatggccaat gaccataata atgacaccag
    11341 ataaaaaacc tttttttgcg ggaacatatt tcccaaaata ttcaaggtat aatagacctg
    11401 gagttataga tttattagag aatgtatctg aaaagtggaa tacaagcaga gatatattaa
    11461 taaaatctgg agatgaaata atagaagctc ttaaggatga ttttggtgta aaaaatactg
    11521 agggtgattt atcaaaagag atgttgagtt catcagttag agtatttaag gctatttatg
    11581 atgaaaagta tggtggtttt ggtaatgctc ctaaatttcc aagtcctcag aatcttatgt
    11641 ttttaatgaa atattatagt atagaaaaag acaaagatgt gttaaaaatg gtggaaaaaa
    11701 ctttagatgg aatgtataga ggaggcttgt ttgatcatat tggatttggg ttttcaagat
    11761 actctactga taaaaagtgg ttggctcctc attttgaaaa aatgctatat gataatgcta
    11821 tgcttactat agctttttta gatgcgtata aaataacaaa gaaagagttg tataaagaaa
    11881 ttgctataaa aactatagat tatgtagtta gagagatgaa ggataaagaa ggtgggtttt
    11941 attcagcaca ggatgctgat agtgaaggtg aagaaggtaa attttataca tttaatcctc
    12001 ttgagataat tgaagttttg ggtgaagaag atggaatttt ctttaataat tattttgata
    12061 taactagtag tggaaatttt gaaggcaaaa gtattcctaa tcttataaaa aataaagaat
    12121 atgagagaca taatgaaaag atagctgacc ttagtaaaaa ggtatttgaa tatagaaaag
    12181 aaagaacatc tttgcataaa gatgacaaga tacttacatc atggaatgca ttgatgatag
    12241 ttgctttaac aaaggcatat agtactttaa aaaatgacat atatttagag tattcaaata
    12301 agtgtcttaa ctttataaat aataatcttg taaatgagtc aggtagatta ttagctagat
    12361 atagagatgg aagttccgac tatttagcat atttagatga ttatgctttt ttaatctggg
    12421 cgtatataga gctttatgag tcaacattta atatgaaata tttagaaaag gctttaaatt
    12481 tgaatgaaag ctgtatcaac cttttttggg attatgaaaa aagtggattt tatatatatg
    12541 gtaaagatag tgaaaattta atagcaaggc caaaagattt gtatgatgga gctattccat
    12601 caggaaattc tgttcaatta tacaatctta taagacttgc gaaaattact ggagataata
    12661 gacttgaaga aatgtcatat aaacaattaa aactgtatgt ggataatgta aagagttctc
    12721 cagctggcta cagcttttat atgctttcat tgatgtttga actttattct actaaggaaa
    12781 ttatatgtat ttttaaggaa gattctgatt taattgcctt taaagaattg ataagtgaaa
    12841 attttatacc aaatgcaact tttttagcaa agaaatacaa tgaagaaaac actattatag
    12901 gttttttaaa taactataga ttgaaggatg ataagattag ttattacgtg tgtcaaagta
    12961 atagttgtag tcaaccaatt aatgatttac aaaaattaaa agatatgatt ttaggagtag
    13021 aataatcata atagtgaaaa aggagatgct ttaaaataaa agtctaatca tctccttttt
    13081 ttgtacaaaa aacagaaaaa gagttcctga gaacacctaa tctatgtaat gattgttttg
    13141 ttaaaaaata aaaatgacat tatagttata taaaatattg tactataaaa aagtagtatt
    13201 atagatgcga tagtattaat cgtttttatt tactctcttt cttctttttg caaattcttc
    13261 aagtgcttca gatttaattt tatctatttc ttcatattcc ataaaattag catcttcttc
    13321 aaagccatct tgttcttcac cattaaaacg atacattttt tttagtaaac tccataaggt
    13381 ctctaattca tctttggtaa attcgtgaaa tacatctgcc ataaagttta ttccagtcct
    13441 actacatgtt accataacct tttttcctaa gtctgtaact ttaacattaa ttgctctctt
    13501 atcatgagga cttggaatta catcaacata tccatttttt tcaagattag caactagtct
    13561 atttatattt tgtttacttg tacccatttt tcttgcaata ttatttaatg ttgtttcttc
    13621 ctctggtaaa tgaagaattg ataaaatagt catatactgt cttgaagtaa gtataccaaa
    13681 atatttatct ccttctattt gtattttgtt tgtaagggta aaaagagttg catatgtttg
    13741 attcattaaa aagagttctt ttagttcatt tgaataatcc atgaaatata tctcctttat
    13801 ttttagatgt tgttttgtcg aaaacagaat atcatgaatt aattctaatg tcaatatggt
    13861 gtcaaaatat attaaagtat tactttttta tgtaactatt aattaattaa attattgtat
    13921 aattttatgt aaaaacttct atgtaccaat gataacttct atgaggaatt aataattctt
    13981 aaatttttta gaaagatttt gacttgaaaa tacttgtatt ataatatata aattaatata
    14041 ctgaataaaa tcaatctgga gggataatta tgaagtgtac aaactttact ttcaaaggag
    14101 aggaaggttt agatatatat acatataaat gggaagatga aaatataaaa aaaccaaagg
    14161 cagttatcca aattgcacat ggaatggctg aaactgcaca aaggtatgag acttttgcca
    14221 aggtacttac taaaaatggt tatatagtat atatcaatga ccatagaggt catgggaaaa
    14281 ctgcaaagat aatagaaaat gttggacatt tagctgaaaa agaaggattt agatgtcttg
    14341 tagaggacat gtatacttta acaaatatta taaagaaaga aaatgaagat ctgccaattt
    14401 atctatttgg acacagcatg ggttcatttg caagtcaaag atatataatg gattacagta
    14461 ataatttatc tggtcttata ctatgtggtt caaatggaaa gcaaggaatc attctaaatc
    14521 ttgctcatct gataatcaat cgtgagatta aaaagtatgg aagacgttct aaaagtaata
    14581 aaataaataa tttgatattt ggcggcgaaa taataagaag aaatgaaaag actaagtttg
    14641 actggttaag tagagataaa gaacaagttg aaaaatatat aaatgaccca ttttgtggag
    14701 tagtgtgtag ttgtggcttt ttttatgatt tagttcaagg gttaaaagaa attgaggata
    14761 aagaaaactt aaaaaaagtg ccacttgata tacctattta cataatatct ggagataaag
    14821 accccatagg aaaaaatggt aaaggagtct taagactaag ggatagatat ataaaattag
    14881 gagtaaaaga tgtaacttgc aaactttaca aagatggaag gcatgaattg ttgaatgaaa
    14941 taaatagaga agaagttttt gaggatataa tttgttggtt aaataataaa atagaaatat
    15001 tgtaataaaa aaatataatg atatatttac tttaaattta tttatatata atatttgaaa
    15061 agtggattat gtatatgcaa agtagaaaag agagagccca gaagtgattt tttgctataa
    15121 tgccatttat tgaataggtg ggtgttatta taaataaatt attaacactc agtattatta
    15181 tcattgtata tggaatttat tttatttaaa attccttgaa agtttaaata tagtaagttg
    15241 tagtaaagag aaaatataaa tatttaaatg ctatttaatg tgaatctatt aattaggtta
    15301 tacttaaact taattaatag attttatatt tcaagacaat gagatagggt tttagattaa
    15361 aatataacca atggttaaat tacagacatt ttgttaactt tccctcgatt tactagtaaa
    15421 tatttttttt atatacttaa attacaacag atgtactagg gaggtatata tgcaaattaa
    15481 aataggtaaa gtcatacagt gtcttagaaa agaacgaaat ttaactcaag aacaattagc
    15541 aaagtttata ggtgtgtcaa caccagcagt atctaagtgg gagagtggaa attcttaccc
    15601 agatatagaa ttattgcctt tattagcaga cttttttaat gtgtctattg ataagctttt
    15661 aaattacaag attgatttaa gtgaagaaga agtaatgaaa atttataaag aattagaatc
    15721 aggctttgct agaattgaaa ttgatttatc aacagaagaa ccaaaagaag aatttagaca
    15781 agatttagaa tctgtaaaaa agctttccaa tatgtatata gagaagtatc ctaaaagcta
    15841 tttattgaag ctaaggatat gttcgctgta tcagatgtat tcatataaat ttggtaagag
    15901 tgaacttaat gacagagtaa aagaaacaac gaatattctt gaagatatag tgagaaatac
    15961 agatgatatc caaataaaag aaacagctct tattatatta tcaaacgcat attgtatgtt
    16021 ggaggattat gaaaaggcag agttgtattt aaatatgata cataagtcaa taggggatac
    16081 aagtgttaat ttagctatga tatatttaaa gcaaaataga cttgaagaag cagaaatatt
    16141 gcttcaaaat aaattgttta gtaatgtatt taatataagc atggattgta agggaataat
    16201 taatgtatac aaaaatcaat acaaggaatt aaagaaaaaa ttggagaata gaaattttaa
    16261 taaaaatgca attgagagag aaatggagta tataaaaaat aagttgctgg ggtatgcaaa
    16321 tctttcttta gaaataaaaa aaatgctcag tgaagataaa ggtgcatttt ttagtatgta
    16381 tatggattat atggaattat ctctaatttt tttatttttt aacatgaaag aagaagcaaa
    16441 aaaagcttta tatagtctaa aagaaatatt agaaaaatat cctatacatg aaagtttgga
    16501 tgtaagtcaa atgagatttt ttgataaggt agagtctaaa aatttatata cttttaatat
    16561 atatacaaac ttgttaattg tacttaatga tgacagttat aatgagctta gagaagaacc
    16621 aatttttaag gatgtaattg aaaagatttt agatatggaa aaaatgttga aaaataaaga
    16681 ataagtaaaa atttaatata ggacttaaag attaatcaaa ttaaaagaaa gtgtttcaaa
    16741 ataaaaattt gaatcacctt ttttgtcggt cttaaacagt tatttagaaa aatagaggtt
    16801 tcaaaataga aatctgaatt atctgtatta ttacacaaaa atagaaacct aaatttataa
    16861 aaatagaata aaatttgtta tataaaatgt aattttgtct ataaaatctc acctgttttc
    16921 ttaactttaa gcgtgaaaaa gaggatgtat catgaatttt ttagttcatg atgcatcctg
    16981 ctttgtatga aatccaatat atctttatta tttaaacaat ggaaaagagt cttatttata
    17041 ttttgagaca tctcttttat ttaattatga agttttatac agttcaataa ataggaatta
    17101 tatttagaaa ctcatgacat tatttcttag atagtgatta aatttatcat attccatttt
    17161 agataacttt tctgggtaag acattacaat caggttatgc ccattaaatt ttttatggta
    17221 tggtggatga atgtgttcat aattatttgt cttatatttt actctttcat agaacgcctt
    17281 gcgataaatt gaaatctcat caattggaag gtcaatttct aaaataatag tttttccttt
    17341 cttatttaaa agctctaacg ctctttgccc atatctatta ttgcgaattt ctggaaaaat
    17401 gcagaaatgt tctacataga taaagttatc tgtttcccaa cataaaatta tgccgacaaa
    17461 ctggttttta tcataaatta aattgaattg atattcttta tttttcaaaa tttctgcttg
    17521 caaagaagat tttctttgtt tatgaaatgg gaaactaatt ttatataatt ccatagcctt
    17581 gtaatataat ttattttcac tggttcttaa ccgttcaaat ttcactttac tcctcctaat
    17641 cacgaaaatt ccaatttatt tatgagttca ttttaccata gtcagtattt gcatacaatg
    17701 tgttttaagt tgagtgtcat ttctttcttt tatttatgct tatatctcat agtctaagag
    17761 ataattgctt aagacataac taaaaactat tatattaagc tttgataatc taaaaacagt
    17821 atatttatgg actt
//
LOCUS       DAYAOX010000072        17806 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.072, whole genome shotgun sequence.
ACCESSION   DAYAOX010000072 DAYAOX010000000
VERSION     DAYAOX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.072"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            567..1427
                     /locus_tag="ACGI57_001843"
     CDS             567..1427
                     /locus_tag="ACGI57_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9716922.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKTLNIKASDYLSLTLLDHLEF
                     LLKRCKKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSE
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1417..3288
                     /locus_tag="ACGI57_001844"
     CDS             1417..3288
                     /locus_tag="ACGI57_001844"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9716923.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQSTTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3308..4762
                     /locus_tag="ACGI57_001845"
     CDS             3308..4762
                     /locus_tag="ACGI57_001845"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9716924.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYEMPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLGKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            5787..6995
                     /locus_tag="ACGI57_001846"
     CDS             5787..6995
                     /locus_tag="ACGI57_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716925.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            7026..8405
                     /locus_tag="ACGI57_001847"
     CDS             7026..8405
                     /locus_tag="ACGI57_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9716926.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            8511..9701
                     /locus_tag="ACGI57_001848"
     CDS             8511..9701
                     /locus_tag="ACGI57_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9716927.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIREETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEVLLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(9829..11157)
                     /locus_tag="ACGI57_001849"
     CDS             complement(9829..11157)
                     /locus_tag="ACGI57_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716928.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCIGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(11775..12989)
                     /locus_tag="ACGI57_001850"
     CDS             complement(11775..12989)
                     /locus_tag="ACGI57_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFG9716929.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            13415..14131
                     /locus_tag="ACGI57_001851"
     CDS             13415..14131
                     /locus_tag="ACGI57_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFG9716930.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            14266..14892
                     /locus_tag="ACGI57_001852"
     CDS             14266..14892
                     /locus_tag="ACGI57_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9716931.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(15092..16636)
                     /locus_tag="ACGI57_001853"
     CDS             complement(15092..16636)
                     /locus_tag="ACGI57_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9716932.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(16662..17303)
                     /locus_tag="ACGI57_001854"
     CDS             complement(16662..17303)
                     /locus_tag="ACGI57_001854"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HFG9716933.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            17573..17767
                     /locus_tag="ACGI57_001855"
     CDS             17573..17767
                     /locus_tag="ACGI57_001855"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFG9716934.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFD"
BASE COUNT         6581 a         2089 c         2930 g         6206 t
ORIGIN      
        1 tatttatata ttagtatatc ttactagcat ttaaatgaaa ctaaatttaa gtatttatat
       61 attagtatat cttactagca tttaaatgaa actaaatttg aagctttacc tattaatata
      121 tcttaatagt gattaaataa aactaaactt aagactttat atgttaatat atcttaagaa
      181 tttatttgtt aaaatacagt tcattatatt taaaagcatt actatgataa ttcataatag
      241 taaaatagat aatggttcag acaatattat aaatactagt gacggtaaaa tagtatcaat
      301 ttagtatata aaccttgtca gataatcgca taacagagct tgtagttata gaataaaaaa
      361 atatctagga aaccgttgac agatttatat ggatagtata acatatagaa tatgaaatta
      421 aacaaaaaag aattttaatt atggcgacgt agtcataatt ttgagtatgt tactgattag
      481 caggcagaac tcaagtatat atgtgaaaaa tagagggata ctctatacat atatgcttga
      541 gttcttttta ttgaagggag gaatatatga aaataattca gattttaaat aataatgtag
      601 ccttagtaaa aagaggtaaa aatgaaatta ttgtatttgt taaaggaatt ggctttaaaa
      661 aaagagtggg acaagtaata aatgaaaatg aaatagagag aacatatata ctagattcat
      721 acgatatgct agaacacttt agttatctac tacaaaactc agactccaat aatatagttc
      781 ttattaatga aataattgaa tatggtgaga aaactttaaa tataaaggca agtgattatc
      841 taagcttaac cttactagac catttagagt ttttacttaa aagatgtaaa aagaatcaat
      901 ttataaaaag tcctttggta tggaatgtta aaagatttta tccaaagcat tttgaaattg
      961 gaatgcatgc actaaaatta attggtgata gtaagggaat taatcttcct gatgatgagg
     1021 cagtatcaat agcacttcat tttgtaaata tggaggtaaa taaagagtct catgatagta
     1081 caattgtaga gttgagaact ttagcagata tagtttctat aataaaatat cattttaatg
     1141 tagagctaga tgaaacttca acaaattata tgagatttac tactcatcta cagtacttta
     1201 tccaaagaat tatgtcagag gaagcttcaa tagatgaaga taattctata gatttatata
     1261 aacaagtaag tcaactttat ccaaagtcat ttggaagtgt tcaaaagata agaaaatata
     1321 taaaatcaca atttaatgtc gatatatcta taagtgaaga aacttattta atgttgcata
     1381 taaatagggt gactcaaaga ctggaggcaa aatcagatga aatataaaga tttaaatgaa
     1441 aatataataa aacttatagg gggaaaagaa aatataaagt cggtagcgca ttgtgttact
     1501 cgtttaagat ttactttaaa tgataggagt atcgctcaaa cagaagaaat taaaaaatta
     1561 aaaggagtaa ttgatgtagt ttctaatgat gttgcttatc aaattataat aggtactcat
     1621 gttgtagatg tatataacga gtttatgtct atgataggtt taagttcaag tgaatcaagt
     1681 ataaaaagtg aaagaaaaaa gggaattaga ggtatcaaag gtatatttaa ttccatcttt
     1741 gtggtagtct cagagactat gacttcagta attgaagtac tacttgcagc tggtatactt
     1801 gcaagtatat tagcactttt aaatatgtct ggcatacttc agagtgacag tcctacatat
     1861 caaatattag atacattaag aagtgctaca tttcacttct taccagtatt aattgcaata
     1921 gcatcagcaa aaagacttaa tgtaaatcca tatatagcag tagtattagc agttacgtta
     1981 ttatcttcat caatagatgg tgtagaagga ttaagtttat ttggtataaa tttacagtct
     2041 acaacttatg caaacagttt tattccaata cttttaggag tatgggcaat gggaattata
     2101 attgaaggtt tgaaaaaaat attacctaaa gcactacact attttttagt tccagtatta
     2161 tctttaataa taactctatc agttacatta actgtatttg gacctattgg tatgtggatt
     2221 ggtgatggat taaactatgc atgtatgttt ttaattgata cattgggtaa ttggagtgtt
     2281 gttgcactgt atgcagcttt acaaccactc ttaatagtct ttggtgcagc aaactttact
     2341 atgcctatat ctttaaattc tgtgacaaca ttggggtatg acccaatttt tatgggcgct
     2401 gcaacaatat ctgatattgc agtttgtggt gcaatgtttg gatatttctt aaaaactagg
     2461 aataaagaac aaaaacaatt atttggtaca gtaagtttta gtgcacttat gggagttaca
     2521 gaaccttcag tgtttggtgt gtttatgaaa tatagaagac catttgtagc agtaatgata
     2581 ggtggaggta taggtggttt aatagcaggt ttagctcaag ttaaaacata tggaatggtt
     2641 tggggattag ctgcattacc aacgtatctt gttggaggag aggtatctaa ctttacattt
     2701 atgataataa gtgtagtagt atcatttgta gcagcaacag cagcttccta tttactagga
     2761 ataccttcag atgaaaaaga agaagttaag ggtgataatg aaaaagaatt agaactaata
     2821 atcaacgaga acaattcaag aactattagt attggaaatg ttgcaaaagg tcaggtaatt
     2881 gcacttgggg aagttaatga caaagcattt tcctctggag cattaggtaa aggggttgga
     2941 gttataccta gtgaatcaaa atcaactgtt atatctcctg tagatggaac tataactata
     3001 gtatttccaa ctaaacatgc atatggtata gtgactgatg aaggattaga aatattaatt
     3061 catattggga tagatactgt aaacttagat ggaaaattct ttgaaagtct tgtaacacaa
     3121 aatcaaaggg ttaaaaaagg agatgcttta gcagtatttg aatctgataa aattatagag
     3181 gaaggctttg attctactat aataactgta gttacaaata ctagcgatta tttagatgta
     3241 atctcatcaa atgatgaatc agaagaatta ttaactgtaa tagtataaat tttaggaggg
     3301 aaaattcatg aatattaaag atacattttt ctggggagga agtattgcag cccatcaatg
     3361 tgaaggttca tgggatagtg acaacaaagg acctgcaatt atggattttg taactaaggg
     3421 ctcttatgaa atgccaagag taattactga taaaatagaa gaaaaattgg attatccatc
     3481 tcataatgga atagattttt ataatagata taaagaagat atagctttat ttaaagaaat
     3541 gggatttagt gcattaagaa tatctataga ttggtcaaga atttttccaa atggagatga
     3601 tgaaaatcct aatgaattag ggattaaata ctatgaaggt ctgatagata cattaataga
     3661 aaataatata gaacctattg taacactata tcattttgaa ttacctatga atttagtaca
     3721 taaatatggt tcatggaata acagaaaact tatagatttg tatttaaagt acagtgagac
     3781 tgtaataaga agatttgatg ataaggtaaa gtactgggtt acatttaatg agatgaacca
     3841 tattgaccct caaacagagc actcggatat ttttacttat atgatagcag gtattaagta
     3901 tagtgaaata gaaaataagg ttcaaacatt ggcaaatgta gggtataata tgactttggc
     3961 gggtgtaaag gttgcaaagt tggctagaga aatcaatccc aacaataaag ttggatgtgt
     4021 atttggacta aatccaattt atccatacaa ttgtaatcct agtaatgtgt taaatgcatt
     4081 tttagataat gaccgtgatt attatcaaat tgatgcaatg tgcaatggga agtttccaca
     4141 atataaatta aaagaatatg aaaaaaataa tataactatt gaagtgactg atgaagacaa
     4201 aaaagcattt aaagaaggta agttagattt tataggactt aattattatt tttcaagtgt
     4261 atctactcca aaagaaaatt tatcaggtga taaatcttta tttggtggga ttcaaaatcc
     4321 atatttggaa caaagtaagt gggggtgggc aatagaccct ataggtttaa gatttacctt
     4381 gaactatatt tacagaaagt atcaattacc tatcttaatt acagaaaatg gtttaggggc
     4441 atatgataaa atcgaagaag atggaactat aaatgatgat tatagaattg aatatttagg
     4501 taagcatcta tctcaaatga agtctgcaat tgaagaagat aatgttgaat gttttggata
     4561 tttgatgtgg ggaccaattg atttagtcag tgcaactaca ggtcagatga gtaagagata
     4621 tggatttatc tatgttgact tagatgatga aggcaaagga agttttaaga gatataagaa
     4681 aaaatcattt gattggtaca aagaagtaat aaaatctaat ggagaagttt tagatttttc
     4741 tacggatata gtagtagggt aggaataaga taaatagaag ttaggaacta atgttaatta
     4801 gtgtctagta aagcatttaa aatagagtat agggtcttta aagtaaagtg tgtattcttc
     4861 ttaaaagtaa tggcaactaa aattactata aaaaggagaa tacacatttt ttatgtttat
     4921 acttaagtaa atatttagac atattcatag tataatttac tcattatata aaaatagtat
     4981 gatttactca ctatataact ttataaattt tctggaaaaa tgtcagaata tattgacaaa
     5041 gaaaagattt aaaattataa tctatattaa gaatagtttg acaatataaa aacaagtatt
     5101 tttataaata gtgaaattat ttttattgtc aatttaagaa taatttagga ctactataaa
     5161 gataaaggta tagatgtcat tatatgataa attatacttg tatagaaaga attttaaagt
     5221 atggcttatc attgatttaa tataataaat aatttttgtt tctagtaata gttcctagga
     5281 ataagatatc taaattcgtt tagaaatagt ctaatttaca aaggagggga tgcactaata
     5341 gataaaattt ctatataaag aagacttaaa gtgaagtata ttctgataat attggtttaa
     5401 tgggtatatt attatttcgt tttagatatt ttaagaataa tttaagacgt ttataaaaat
     5461 gtattctata tctaatattt aaataaatat aagtcttcca acatgtatac taatatagag
     5521 agtagttgaa gtacaattct aactcgtaaa attatgtaga aagtaaaaat caaaaagaaa
     5581 tctagtaagt tgataactat agttaatatt tagagagtta actctagtta tatttattta
     5641 gatattttag gaataattta ggacgtatat aatatcatca aaaaatatca agctatacca
     5701 tatgtagtta agaataactg cataatatac attttataat tattgtttta ttgataaaaa
     5761 attattatat ttaggaggaa acaacaatgg aaaatttttg gaatccactt acttgcttta
     5821 ctgtatgtgt aatgatttat gcttttagtg agtttttatc taagaaaaca aaaggtgcaa
     5881 tttcaacatt gttatttgta tgtattatat ttttactagg attttggaca aatattttac
     5941 caaaagacat tacaggagca tcagggatat tagctgcaat gagcaacttt ggtatagctt
     6001 tattaataac taacttagga acacttataa atcttgaaga cttatgtcat gaatggaaaa
     6061 cggttgtaat tgcattagta ggtattgtag gaataggagt tggctgttta acagtgggta
     6121 gtatgttatt tggcagagag tatgctttaa tagcagcacc tccaatagca ggttctactg
     6181 ttgctgggat tatcgtaaca actgttgcag aagctgcaaa tagacctgaa cttgctgcat
     6241 ttgtaattct agtattatca tttcaaaagt tctttggtat tccgataact acatattgta
     6301 taaataaaga attaaaattt aaaagagata acggggattt tttgaaagat gagtcaaaaa
     6361 gtaattttaa acttccaagt atgagaattt ttaaagaaga tttaggtaaa aaatcatcta
     6421 ctctagtgta tcttgcaaag ctttcatttg ttgctatgat tggtaatttt gtaggattgg
     6481 ctactttaat accaggattt acccctgcaa actattattt aaatcctaat atagcatgtt
     6541 tgttagtagg gttgattttt acacgaatag gttttttaga aaaaggaatt ttagataaat
     6601 ctcaatctaa tggaattata atgtttggtt gtatgttgat gttacctgga ggacttgcta
     6661 aggtaactcc aacagcattg tttgggatga ttgttccaac tataggaata cttgttgtag
     6721 gttctgtatt tataatatta tttactgcaa ttgtaggaaa actattggga tattctccaa
     6781 gaatgtcagc tgcaattggt gtaacttgta tgctagctta tcctgcaaca cagataattt
     6841 ccaacgaagg tattgatgct atggaaggaa ctgaagaaga aaaacaaagg gcaacagatt
     6901 atttattacc aaagatgatt ataggaggat ttgttactgt aaccattgca tcagtggcat
     6961 ttgctagtat aataggtcca attatatttg gttgatagta aatatactta taggaggaat
     7021 aggaaatgat aggaagtata tctataaatg agatagataa aaaaagattc aaacttgatg
     7081 atttaagtaa gaaaatatgg gaaaatccag aaaaagcatt taaagaattt aaagcatgtg
     7141 aaaatacagc aaactttcta agaagtgaag gatttgatgt ggaagtaggt gtaggaggtt
     7201 tagccacggc tattagagct agttttggtt caggtaaacc agttataggt tttatgggag
     7261 aatttgatgc attacctggt ttaaatcaaa aagtttcaac aaagcaagaa gcatttgaat
     7321 tgggtgcata tggtcatgga tgtgggcata atttattatg tacagcacat gtaggggcag
     7381 tagtagggct taaaagagag atgatagaaa ataacttaag tgggactata gtattttatg
     7441 catgtccagg agaagaacaa ttaacaggta aaggatttat ggctagaggt ggagcgtttg
     7501 aagggttaga cttagctata aattttcatc caaataaaat tagtgaagct acaattggta
     7561 cttcaacagc tgttaactct gtaaagtttc actttaaggg aaagactgca catgcagggt
     7621 cagaccctca aaatggaaga agtgcactag atgctgtgga acttactaat gtgggagcta
     7681 attatttgag agaacatgtt acttctgatg taaggattca ttatacaatt acagatggag
     7741 gagtagcacc caacatagtt cctgataaag catcagtatg gtattataca agagctttaa
     7801 gtagagaagc tgtagaaagt acatatgaaa gacttgtaaa ggtagcaaaa ggagctgcaa
     7861 tgatgactga aactgaagtt gaagtagagt ttttaggagg gtgttataat actttaaaca
     7921 atcatgtttt agcaaatctt gtatcagaat gtatgaatga gattaaacaa gagccttgga
     7981 ctcgagagga attggatttt gcagctgaat tagatagaca aagcccacaa caatataagg
     8041 ctatgataag taaatataat ttgccagaag gaactcatct atattatggt ggaggaaatg
     8101 ttacaaactt taatagttat ggctctactg atattggaga tgtaatgcat atagttccga
     8161 cagcatactt ttttacaggg tgtacgaatc taggagcacc aggtcatagc tggcagttta
     8221 catcttgtgc aggaagttct ataggagaaa aaggtatgat atatgcatca aagattatgg
     8281 ctatgtttgg agctaaaata ttaaataatc cagagatagc taaaaaggca aaagaagaat
     8341 ttgataaatc tatgaatggt aaaacatata aatgtcctat tccagatgat attccaactc
     8401 cttaaaaaga catgaaattt aatatataaa attataattt ttatatcagc atcatttcat
     8461 tactaaaaga aagtgagatt ttgctgacta tctgattagg aggaaggaat atgaaaaatt
     8521 tttttaaaga agcgcagtca atagaagggg aaattattag atggagaagg tatttacatg
     8581 aaaatcccga aattggttgc aatctaccga aaacagtaac ctttgttacc agtactttag
     8641 aaaaaatggg atattctccg aaattgacgt ctaataatgg aatagtagct gttttagaca
     8701 gtgaaaatgg tggaaagacc ttattattaa gggcagatat ggatgcactt cctattaggg
     8761 aggagacgga tttacctttt aaaagtaaga ataaagatgc atctcatagt tgtggacacg
     8821 ataatcacac agcaatgctc ttaggagcag ctaaaatact tatggataat aagcacttac
     8881 ttaaaggtag agtaaagttt gtatttgaac cagatgaaga gactaccaga ggtgcaaaat
     8941 caatgattga tgatggtata ttagaaaatc ctcaggtcga tgcagctatg gcatttcaca
     9001 gtatggtggg taaatttctt aacacaggtc aagtagctta ttcacgtggt ccagctatgg
     9061 catctgcgga tatatttgaa ataacagtag aaggaaaagc aactcatgga gcaagccctg
     9121 aatttggagt ggacccaata aacataatgt cgcatataca aatagcatta aacactatac
     9181 tttcaagaga aaaacctcag aaagagcctg tagttttaac tattggtatg tttaatgcag
     9241 gaaacgtacc aaatgctata ccaaataaag catctatgtc aggaacttta agaacatttg
     9301 accaatttgt tagagaaaaa ttaaaaaata gaattattga aatatctaca ggaatagcta
     9361 aatcatttgg aggaaatgct aaagtggagt ttaaaatggg gactcctgca gttataaatg
     9421 atttggatgt aggagatgag attgtatctt atgttaaaga agttatagga aatgaaaatg
     9481 taatagactt tccatctgca atgggttcag aagatttttc agaggtttta ctaagggttc
     9541 caggagtatt tttatggatt ggaatgggaa gtattagtca gggatataat tttggtatgc
     9601 ataatcctaa agcaattttt aatgaagagg gattagttta tggttcagct ttatttgctc
     9661 attgtgcagt aaaatggtta gaaaataatt ttagtagata ataacataaa atattattaa
     9721 atatcataaa aggtatatct taaaaagatg caaagatata ccttttagaa agtcatatat
     9781 aattttactt aatattagat gataaaatat ttttatgcat tttggatttt aatatttatt
     9841 ttctgatata aataatttgt ataccctttc aggtctgcct tttgttgagt tttgtttttc
     9901 atataatata gtagcgtttc cagactttaa gagactactt agaaatctat tagcagttct
     9961 aactgttaca tttaaacatg aagataattc cttaggagta acttcatctc tttgtagaat
    10021 ttcagtaaca gatataatct tttgaatagt aagagtagac aatttagaac ttttggcaat
    10081 tgtatttata tgtggagtta ctttatttga aacagtaagt aacttatcac acagtaaagg
    10141 tcctattgtc tcataatttt cgttaactaa gaaactgtaa tttaaaggat ttattttaga
    10201 ttccttatta gcacttatgg cactagacat agcttgcttt atatcattac caattccata
    10261 tccaatacat actttaatat ttgtactatt ttcaatagat tcttttatat aacatgcagt
    10321 aaaattttta gttaatgatt taatcgtttc acatttagtg aaaattttaa aagaattact
    10381 atttttttca attataaaat cggttaattt atctttttta atactattta atattttata
    10441 aacttggtct agagtagagt catcataatt tgtatcttta actgttatat atattacact
    10501 tggttgattt cccttcattc tatgtaaatg aatatctttt attaagttat taaaagtatt
    10561 ttttaataaa tctatatcag ggtatatgaa atgaaagttt actcctgcat tttgtaacat
    10621 aggaacaata ctactaaaac gagtagtaga aaaatcgatt ttcccttcat tccatagatt
    10681 aatatgtttt tttgcaatct ttttttcaag ttttgctatg tcagataaac tcattttaga
    10741 tgcattttca tcaatactat ccattaagtc tccaaatgtg ccatttgata aataatatga
    10801 tatatctttt ggattatctg cccagtctgt taagtcaaag tatgctctgg aaaaatctaa
    10861 tgtattatat ttaaagataa gctttagaaa catatcatag taactttgta agtcagtacc
    10921 aaaatcaact agaggttttt taatagttcc agcctttttt gtaatagctt ttttggggat
    10981 ggggccacta actgcaaatc catcaaacct atcttctatt tctaaatata aatctccaac
    11041 atgagaaaaa tctctataga tatatatttc aatttctgca ttaatattta acttttttat
    11101 agaattatct acaaactctt ttaaaaattc ggttgttatg actgctattt tcatcataga
    11161 ttacttcctt ttcaattatt ataattattt tatattaaat attacattaa tctattgttc
    11221 tagttaaaat cgtatttcaa ttaaattcaa ttaaaataaa taactcttta taaaaagcaa
    11281 ttatttaggt agaggaatat ttgctaactt taatagtctt tgttaacttt aattttattt
    11341 aagttattat agtcttacta attttataat tgtagattta actactttat gttcttttat
    11401 tgtttatgta gttgatttag aagttatttt aaccatttat tttaattgaa gttgtttgac
    11461 ttttaaatcc tttgtgtaat ctttgttttt ctagccatga taaaagtatt tctatttgta
    11521 tcactatata taataatatc atgacaaaat atttattgtc taactaatta taatctatcc
    11581 aaaatgaaaa tgggtgtgag atattattac aacaaattgt attagtatta aaaagattaa
    11641 aaaattaaaa aactaaacag ttaaataatt aaacgattaa gtgactgtca tataacaatt
    11701 tataatttta tttaacagcc acttaggtat ttaagagcga tagaattgag tgtatatcaa
    11761 attataataa tttcctagta aataattgaa aaataaggta gtccattttt atatttgata
    11821 tctggtttac cttgaataag tggatataga taatttaggg cttcatcagt tatataatta
    11881 cattcttcat ttatccactc tttaggaaaa tattttgtct tgttagctat tttagaaata
    11941 tcagtatgac taacttcata cttataagga ttattgcatg ttctattaat gatagccata
    12001 actcctgtat atcctttttt agatagttct atcccatatg caccaacttc agctgcttgt
    12061 tttaaatcag tatctgaagc taaatgcata gcacatcttt gtattacacc aagttctaag
    12121 gtttttactc ttgttgtaat tgatgatgat actataagat tctttagata gttgcttact
    12181 ccacctaatt gggaatgacc aaatttatca tgagcagaag ttttttgttc gcatataaag
    12241 ttgccgtatt tatcccttat accttctgat gcaactatat aagccttatt tttttcttta
    12301 aatatttttc taacatcttc tataaactta tcttcataga aatcaatttc tggaaggtat
    12361 attaaatcaa caagtggagt atcattaagt ctagctagtg aagaagaagc tgctaaccat
    12421 ccagtatctc tacccatagt ttctactata aatataccat tatttttgta tatagaaaaa
    12481 tctaagtatg tttccattat tagtgtagaa ataagctttg cagcagaacc aaatccagga
    12541 cagttgtcag ttcctaataa atcattatct attgttttag gaatacctat aaattttatt
    12601 ttacaagtag tgtttttgca gtattgagaa aatttcatca ctgtgtccat agaatcattt
    12661 ccgcctatat aaaataatgt atctatttta tattcatcta gtatatttat aactctatca
    12721 taatcataag aatttatttt ataatcagcc agtttgtatc tacaagaacc aagaccagac
    12781 gaaggtgttt gctttaaatt taatacatta tctttgttta aggagtctga atctattata
    12841 tttttgttta atataccttc aatcccattg ataccaaaat atactttatc aaagtattta
    12901 gtttctaaat ttttttcata aataccaact gcagaagcat tgataacagc tgtagggcct
    12961 ccagattggg caataataca gtttctcatt ttacatctcc ctttcattaa tattgttatg
    13021 aatggtactt tttatcgagt aaatttatat ttatagtctg attatatatt aaatgaacta
    13081 gctatatcaa ttgagttagt atagacaata aatgccataa gaatgtggta aaaaactaac
    13141 tttttatata caattaatgt ctataattta ccacaatata aaggtacttt taaggagtag
    13201 tcaaagtgtt gtgaaaaaaa atatatcagt gtaatattaa tttacaaagc aaaataagtt
    13261 cacagttaac aaaagcagaa taagttcata aaagaaatat atacagcaat aaaagtaatg
    13321 ctaaataaaa gtaatactgg attaaaaaat attaaaaaaa tattaaaaac taatatttaa
    13381 tactaattaa agtaaaatat ggaggtatac aaatatgaaa cacataaaaa tagcagcagg
    13441 attagcacat gtaaattacg gtcatatatc agccgtagtt aaagaagcaa tggatgcagg
    13501 tgtagactac gtacattcag atgcagcaga tatgcatgac ttaaaaaata tgcaacttat
    13561 gggtggtcat caaataatag aagcaataag accggtgaca gataaaccaa tagaatgcca
    13621 tatatacaca agagattgtg atagattatt tatagaaaaa atagcagcag caggatgtaa
    13681 cttgttgata ataccagcag aacatttttt aggtgctcct cttgcataca taataaatta
    13741 ctgtagagaa tttggtatga aagtaggatt aacaattgga tgttacacac cactatgttt
    13801 tgttgatgaa gctatatatg atatagatag attgcaaata gtcgttcatg gtgttgatga
    13861 aactgatgga aaagataact ggggatggag aaagagtgct gttgatttag taaaaagagc
    13921 tagaaaaatg atagacgaaa agaatcctaa gtgtgaatta gcaatagatg gtggactaag
    13981 agcagacaat atggagccat taatagaatg taatccagac gttgtaatat tatcttcagc
    14041 aatattcaag gataaagatg gtataacagc aggtgttaag aattgtagaa aagctataga
    14101 tgaagcagct acaaagtttg gtttagaata agataataaa ctatgataaa agagatgctt
    14161 tcttaatgtt ttaaggaagc attaatttta tgaaaaacta caggaagcta gactaattta
    14221 attgattttt tatactgaaa caatataatc ataggaggtt attaaatgaa aaaagaaatg
    14281 ataaaagatt atttactgat aacaatatca gcatttttaa tggcattagc agttaatttt
    14341 ttctttgcag aacatacatt agctccagga gggataacag gattatcagt cgtacttagc
    14401 acattcttat caatgcctgt tgagaatata tcattaggaa tatctatgcc acttttagta
    14461 atgggattat tatttttagg gaaaggtttt ggaataaaaa cattatatat aactcttatg
    14521 ggcccattat tcttaaaagt aataccacaa actcacataa ctgacaatct attagtagca
    14581 ggagtgatag gtgggttatt ggtaggaact gctataggga tagcaataat aaggggctgt
    14641 agtactggtg gaactgattt agttgcaatg cttattaata aggtaattaa gtttctgaag
    14701 ttgccagtta tattatttat gctagatgga tttataatag tagcgtctgg aataataagt
    14761 aagaatttta tgatatctat atattcattg atatctttat tactcataat aaaaacaata
    14821 ggatttgtaa ctacaaagtt tggtaagaat actgaagaaa ttaataatat aaataatgct
    14881 tgtgctcaat aaaaataata aaatttactt taaatataaa agtttaaaaa atataaaagc
    14941 ttaaataaaa agattaaata taaaaaatga aggtgtctca aaatgaattt tttagttcat
    15001 gaggcacttt ttctatgaaa tcaaatatat ttttatcatt tggacaatga aaaataggtt
    15061 tatctctatg ttgagacaag cctatttttg tttatttata agataatatt ttattttttt
    15121 cagaatatag tttttttata attgtactaa gtctagaaat atcattctta cttaaaagag
    15181 catttatatt tatcagttca gcattatgtt caaagtctac aggagcagtg gttagtatta
    15241 agtccacatc atcaaagttc gtaaatgaaa tagaagatag aggtatagat ttaactatat
    15301 taatctgatt aaattcaaaa cttaactttt ggactaataa attagataca ccatcactac
    15361 agtgacatat taaaatagta tttaggggag atttgcttct atctattgct gatattaaat
    15421 ataaagcaag atatcctatt tcgtcatcat tgacttcata attcatcaac tgtttgataa
    15481 cagtattaga ctctttagca gccttataag tgtaaggaaa tgttctcttt atatcattta
    15541 atatgggatt tgtaatagta aacccatagt ttattctgtg tagaactgat ttaaggtgat
    15601 tagcaagaga ctttattaat attaaatcat cctttaagtt taaatttagt atacttcccc
    15661 aatgaacagc tattgcttca gatagattat actctgtaat ttcttcattt ttaagaggtt
    15721 caagagtagt agttttcttt attgatttaa gtaatccttc tatatgaaga aggatataac
    15781 agacctcttc atcaataaat tctacgtcaa aagcttcact taggttagca cataaaattt
    15841 ttgatttttc aaagtattca tgttctaata attctctaaa aaaaccttca gataagttaa
    15901 tatatctttt aattgaaatt cttttaatac atatagctat atgtattaag aagtttatat
    15961 aatactcatc actaaggtta cttttttcta cttgtaaggt gtgtagagtg atttgagata
    16021 atttctcaaa atcatagtta aatagctcct taaatatttt tgttgatggg gtatctttgc
    16081 attcattaga tttcgagaat aatatttctt taagttcagt ataactttta ttaaccgtag
    16141 caagttcaaa aattgctttt cgaatatctt tctcttttcc ctctaaatat actccattat
    16201 tggtttttct aactagagtc actttaaagt tttgcaaaaa attttgaata actaccaagt
    16261 ctttatgcac agtagctcta ctaacatgta gttcattgca aatatcacga atcctaaact
    16321 tagaattttt aataaataat ttacttagaa tatacctttt tctatcttca ggagagtatg
    16381 catatataca atttgattta tcattgatat ctctaataac tttaagtttt atatcttcat
    16441 tacctagaat aataacccct gaaccagttt ttttatctag ggataaatta aactctaaaa
    16501 gccattcatc taaaataaca aggtcattac gaatagtttt attactaact tttaattctt
    16561 cggcaatagt atctacagta atatagtctg agctgttgag taataattca gcaatatcta
    16621 ataatctttt gttcaatgca aaccctccta aatcttagtt actatttatt aaccttggac
    16681 caatctcttt caaaagtatt ggcaccatct attgtaagct ggttaaaaga agctttttta
    16741 aataaatcat atgttacagc tgcataatca catccacaaa gagatgcttg agttaagtgg
    16801 aatacatttt taatagaagc tgcaacaact ttagtattat atccatttat tgatataagg
    16861 ttacatattt ctgtaaggtt ttttatacca tcattcccat tttcatcatt tctacctata
    16921 aaagggaata ggtaatcagc acctgcattt atagctaatg atgcttgatt tacattgaaa
    16981 atcaaggtac atgcagtttt tacaccttta ctatttaata tgtttataag ttctaagcca
    17041 acttcagaga aatttatttt tattatagtg tttttattta tatttaatat cttatcacat
    17101 gaattaatta attcgtataa attatttcca atgacttcag cagttaatat ttctggattt
    17161 aaattagcac atgtctcaat aaattcatgg aagtttactt tctctttaag atacatagat
    17221 ggattagcgg ttattccctt aaatccatac tttagacact tttgaatttc atcaagatta
    17281 gcactgtcta tcaaatattt cataatttaa ttctccttta agctatttgt cataaaaaag
    17341 tgtatatact ctatttttat tattaagtca attatacaat aagattgatt ataaaattat
    17401 aacatgggta cttaataaaa tttacataat gatatggtaa taataaaaca ttgattgtct
    17461 aataattacc ataagcttta gtaaataaga gttaacagtg aaggtattgc cttaaaaatc
    17521 ctatgatatg tataatttga atatgaaggc aataatttaa gggaggaaaa atatgattaa
    17581 cttttatcaa ccatcaagaa taaattttgg acaaggaact ttaagtgaat tacctagaat
    17641 aataggaaaa tatggaaaga aatgtttgtt agttactact ccaaatgtag agccattaga
    17701 caagttatat aaaagagtta cattgaattt aatagaaaaa ggtatagaag ttgtacactt
    17761 tgattaaaaa atagctacgc acactcgtga ctttagtcat gagtta
//
LOCUS       DAYAOX010000073        17670 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.073, whole genome shotgun sequence.
ACCESSION   DAYAOX010000073 DAYAOX010000000
VERSION     DAYAOX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.073"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(8..772)
                     /locus_tag="ACGI57_001856"
     CDS             complement(8..772)
                     /locus_tag="ACGI57_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFG9716935.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            1046..1720
                     /gene="fsa"
                     /locus_tag="ACGI57_001857"
     CDS             1046..1720
                     /gene="fsa"
                     /locus_tag="ACGI57_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFG9716936.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVGKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1842..3236)
                     /locus_tag="ACGI57_001858"
     CDS             complement(1842..3236)
                     /locus_tag="ACGI57_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFG9716937.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3314..4660)
                     /locus_tag="ACGI57_001859"
     CDS             complement(3314..4660)
                     /locus_tag="ACGI57_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9716938.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      4910..5012
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5106..6482
                     /locus_tag="ACGI57_001860"
     CDS             5106..6482
                     /locus_tag="ACGI57_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFG9716939.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(6596..7741)
                     /locus_tag="ACGI57_001861"
     CDS             complement(6596..7741)
                     /locus_tag="ACGI57_001861"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFG9716940.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIILDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            7994..8701
                     /locus_tag="ACGI57_001862"
     CDS             7994..8701
                     /locus_tag="ACGI57_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716941.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            8748..10343
                     /locus_tag="ACGI57_001863"
     CDS             8748..10343
                     /locus_tag="ACGI57_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9716942.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLMVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            10446..11528
                     /locus_tag="ACGI57_001864"
     CDS             10446..11528
                     /locus_tag="ACGI57_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HFG9716943.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            11677..11838
                     /locus_tag="ACGI57_001865"
     CDS             11677..11838
                     /locus_tag="ACGI57_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HFG9716944.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            12179..13447
                     /locus_tag="ACGI57_001866"
     CDS             12179..13447
                     /locus_tag="ACGI57_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFG9716945.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            13642..14430
                     /locus_tag="ACGI57_001867"
     CDS             13642..14430
                     /locus_tag="ACGI57_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFG9716946.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSIRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            14430..14879
                     /locus_tag="ACGI57_001868"
     CDS             14430..14879
                     /locus_tag="ACGI57_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFG9716947.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            14883..17147
                     /locus_tag="ACGI57_001869"
     CDS             14883..17147
                     /locus_tag="ACGI57_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFG9716948.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            17287..17598
                     /locus_tag="ACGI57_001870"
     CDS             17287..17598
                     /locus_tag="ACGI57_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716949.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         6584 a         2073 c         2814 g         6199 t
ORIGIN      
        1 attaaactta taaaacattt acattaaatt ctgataaaat atctactata tcaggagtag
       61 gcgttttttc tgtaattata gaactaatat cttctaaatg agcaaacttg aaatttccat
      121 caaatttaaa ctttttatct tccataacta taaatacttt tttacttgaa tttattatag
      181 ccttcttcgt aagaccatct tcaatttcaa aagttgtaag tgaagagttg aatacatcta
      241 ctccacaact tcccataaaa gtcttatcaa acttatattt cgatataaac tctatagttg
      301 gagtaccgac aaaaccgtct aaactgagat ttaataaacc tccagtggaa attatattta
      361 aattatttgg ttttgaagta actacattta gtatatccag catatttgta acaagtgtta
      421 cttttttatt actttcagct aacaacttcg caagcaagat gttaattgta gagatatcta
      481 aaaatatagt ttctctatct tcaattaaat taaatgcttt ttctgctatt attttctttg
      541 ttggaatatt aatttctttt ctgtcttttg tatcctgatt ctctagtgtt tctctagcta
      601 gtacagctcc tccataaatt cttcttattg aagaaatctt ttctatattt tttaaatctt
      661 ttcttataca atcttctgta acattaaatt tagaactgag ctctttgaca gtaactctac
      721 cttctttttt taggatattt aatatagctt ggattctttc ttctgcaaac attttctacc
      781 tcttttcgca atatagtatt atagtaaata ttataagata aaatacaata tatcaactat
      841 tttaacatga aatacaaaat taatttaata taaaaagtat gttttttcta actatatatt
      901 tagaatttag taaataaaaa aattataaaa ctaagaagga aaagtaaaac aataataaaa
      961 aataaaaaac aataagcaaa atgttgaaaa taataccaaa tcaatatata ataagaataa
     1021 agaaagatta aagggggata ttttaatgca actaatctta gatacaggta atgtggagga
     1081 gattaaggag ttatgtactt gtttaccaat agatggtgta actacaaatc caagtattgt
     1141 ttcaaaagaa aaaaagaatt ttaagcaact tataaatgaa attggtgaga ttataggtga
     1201 ggatatgcct atacatgctc aagtgttaag tacaaagtat gaagaaatat tagaagaagc
     1261 attatatata agtagtttga gaaaaaatat atatgtaaaa atacctgtaa cacaagatgg
     1321 tttaagagct ataaaagacc ttcacagaaa aggtgtaaaa ataactgcta ctgcaatatt
     1381 cactgctcat caaggttttt tagctgcaaa ggctggagca aattatattg caccttatgt
     1441 aaataggctt gataatatat ctggtgatgg agtagctatg gtttcagaac ttataaaaat
     1501 aatagatact tataaaatgg atacaaaagt acttgcagcc agttttaaaa atgcacaaca
     1561 agttatagaa ttaatgcaac atggggtgca tagtgttaca gtaccatatg atatttgtaa
     1621 atctatgatg aatcatccat taactgattg gagtgtaggt aagtttatag aggattggga
     1681 gaatactttt ggaaaaggaa gtaaaacaaa taatatttag tattatggta aaagagtagt
     1741 tttgtccaat ttcacaagaa cttatataaa aagatgaaaa tatgaatttt ctttttaata
     1801 atttcgttat tatcttaagt taattcatat tttcatcttt tttagatttc tagtcctata
     1861 aatttccacc aagggaatgt tatacatatt attataaagt aggttaaagg aatcaaagtt
     1921 aaaccgtatt tgaaaataac cttgctatta tacagatttt caccatgacc aaccattatt
     1981 gtagcatgat gaaatggtaa tatgtactgt atgcttacta taatgtagac aaatagacat
     2041 aaagtaacac tattttcctt tagtatatgt aaacttccaa gtaacggtat tactacagat
     2101 gatgtagtga cagcacttcc aagacatata ttcaagatta ttgtgagagt tatcaaaaac
     2161 aatattgaaa taatttctga agagtttggt ataaacttta tcaaataatt tcccattata
     2221 tctacaactc cagagtactt taacactcct ccaattgaaa atgctgctat aaaaaaaata
     2281 aggaccttcc aatttatttc ttttaaagta ctaaatccaa ctatttttct tagtaataaa
     2341 agaagtacac aaattgacat tataattaat gtgtttatgt tatgtatgaa ctgagttaaa
     2401 aacataaaaa acattattcc taggcataaa aatacgtgtt tttgctcttt gtttagcaaa
     2461 ttaaattctt tttttaaatt agaatatgaa aaatgaagtt tattaccctc aagattttca
     2521 ttagaaataa attttattga atgcaattct tttttaaaga caaagataaa taaaaaataa
     2581 gttataaaac aagtaattat agttggaaca gacatataaa aagcccattg accccaattt
     2641 atattacaat ttccaagttc tagtacaaca taatttagaa gggtgtctcc atttacaaaa
     2701 aacattgatg taaaggtaga tgctgtaaat atactaaata aaagtataga ttttgaatct
     2761 tcagttatct gttgttcttt taaaaactcc ttgtaaaaag cagatacaag aataaccctt
     2821 ggaaatggtt gtgggataaa aaatattaaa agtataccta aaatatacga taaaagaatc
     2881 atttttttag gggtatttac agtatttaaa attaacttct cagaaataat ttttgcgaca
     2941 cctgtttttg taactgcatt agtaattatg tatgcaagaa ttattatgta aaaattttct
     3001 gtaaataaaa actgtaaaat aacctttatt ggtgcaatgt taaatataaa cgctagaatt
     3061 acaaaaaaca atgcaacaaa agttttttca actgagtcta cagcccatat caagatagtt
     3121 aaaaaagata agcatataga aaattttatg ttgaaactgt aattaagaga gaagtagaga
     3181 attatggtag ctataacagt catataaaat tgttttggct taagaataat acccattttt
     3241 atattcacct ccataattta ttatatcata atggaggtga atacaatgaa tgtgaataca
     3301 ttttataaaa agattattca ttgattctat gatttacaat agctttagtt ttccaatgtc
     3361 ctttcttata gtagataact gtaacaataa gacctaaagt ccaaccaaaa ggaataccca
     3421 tccaaattcc gtctgttcca aaaaatttag aaagcatata agaagctgga agtcttccaa
     3481 gccataaaga tactattgag cttataagtg caaacatgga atcaccagca cctttcattg
     3541 cacttgaaag tacaaaagaa gttccaataa ataagaagaa tggtcctatt ataaataagt
     3601 aagaagagcc aattttaatg acatctttat cagtattgaa tagagcaatt agattttccc
     3661 taaatagcaa aattaaagca ataactgtaa ctgctaaaac taaggtcatt tttatagatt
     3721 ctcgaatgcc ttttttaact ctttcatttt ctccagcacc tatgttttga gcaacaaaag
     3781 tagaaacagc agaaccaaga ttcataatag gtaagcttat aaaattttct atcctaagac
     3841 cagcaccaaa agctgccata gcagatgtac cataactatt aactaaaaat tgaagagcca
     3901 tatttccaat tgaaaacaac atttgttgaa ctccacttgg aagtcctaat ctaagtccat
     3961 ctttaaatat tttgttatca tatacaattc ctttgatttt aaatttaagc acttcatgtt
     4021 ttttatttaa ataaattata cttattataa atgatactgc ctgagccatt atagtggcaa
     4081 gtgcaacgcc ctcaacaccc attcttagca ctacaataaa agttaagtct aaaactatat
     4141 ttagaattgt ggctattatc agaaaataaa gtggagttac tgaatctcca agtcctctaa
     4201 gtattgcact tactgaatta tatccagcag aaaataatat tccactaaat attatagtaa
     4261 gatacaattt tgattgagca aaaacggatt ctggtgtatt cataagctta agcattgggc
     4321 caccaaatac tattccaaga atacttatta atatagaacc aataaataaa agtatatatg
     4381 tagtatcaat agtagctttt acctttttta aatctcctga gccataaaat tgtgacacta
     4441 aaatagaaaa tccaaggcta acacccataa gcgctgccac caataaaaac attatagggt
     4501 tagctccaga gactgccgcc attgcttctt ttccgataaa tctaccaact attatactgt
     4561 cagctgtatt gtatagttgt tgaaataaac taccaataag cataggcatg gcaaaaaaga
     4621 atatggactt tccctcatga cctgtagtta aatctttcac cttattcctc caaataaaaa
     4681 aattattaat ttattatagt ataatcttaa cataaacttt atatccaaac tataaataaa
     4741 ttgtatcaaa atatcatata tttttttaaa atgtaaagaa tataattata atttgaattt
     4801 aataaatcaa tatttaatat aatcgaatat taatacaaaa tcaatacaat atatacgtat
     4861 atattgacaa aattaattat ataaattaaa ataatattcg taaagaaaaa ttcatgtttt
     4921 gtactgtata acatataatt ttgacaatat gggtcataag tttctaccgg aataccgtaa
     4981 atattctgac tatgtatact attatttagt taattaaata tttaattaac tgaattatga
     5041 aggatacggc tcagcttatc aactactgtg tcgttttttt gtaaaattta ataggaggtg
     5101 ttgttatgca aacaacaaac agtagtacta tgcagaaact ttttccaatt ttgactaata
     5161 aaaatgtcga catgaagaag gaaataattg caggagtaac tactttttta actatggctt
     5221 atataatagc tgtaaatcca aatattttat ctgagacagg catgcctgca ggtgcacttg
     5281 ttacaggtac ttgtttagca gcagcatttg gatgtatttt aatgggtgta gttgctaacc
     5341 ttccttttgc tttagcatca ggtatgggtc ttaatgcatt ttttgcatat acagtagttt
     5401 tacaaatggg tgttccttgg gaagtagctc ttacagcagt atttgtagaa ggtattattt
     5461 ttatagtatt atcagtaagt ggagttcgtg aggctgttgt aaatgcgata ccaaaaaata
     5521 tgaaattagc tgttacaggt ggtataggaa tatttattgc acttataggt ctagttaaca
     5581 gtggaattgt tattggagac caagccactc ttatcaaaat gggtagattt acaccagcag
     5641 taataataac ttgtgtagga ttaataatta ttgctgtatt agataaaaaa agagttaaag
     5701 gttctatact ttttggaata gtagtgagtt cattacttgc ttgggcattt gcttttataa
     5761 atccagagta tgcacaaaaa ttaggtatat atttacctgg tggattattt aaattcgaaa
     5821 gtttagcacc aatagcaggt aaaatagatt taggatatgt actacatcca acaaatatag
     5881 gtggtttttt agtagtagtt tgtacatttt tatttgttga tttttttgat acagtaggta
     5941 cattagttgg tgtttgttct aaagcaaaca tgttagatga aaaagggaat gttcctaatg
     6001 taggtagagc cttacttaca gatgcggttg caactacagt tggtgcagga cttggagtat
     6061 ctacagttac aacttatgtt gagagttcaa caggagttat cgcaggtgga agaactggat
     6121 ggacagcaat aacagttgga attttattct tagctgcaat gtttttctct cctgtattta
     6181 tagcaattcc atcatgtgct acagcaccag ctttaatata tgttggatat ttaatgcttg
     6241 gtacagttaa ggatatagag tttgataata taactgaagg tgttccagct tttgttacta
     6301 ttgcttgtat ggcattaaca tatagtatag gtgatggttt aactttaggt atattaactt
     6361 atgttttcgt aaatatattt tataatatat ttggagctaa aaaggcagaa gacaaaaaac
     6421 atgtttctgt agtaatgatt gttctggcaa tattatttat aataaaactt tggctactat
     6481 aataggtatt atttaaaatt ttaatttaat ataaaataaa gggctgtaat ttattatcaa
     6541 ttgtgatgat aaactacagc tcttttgtat ttgttgtatt tttgtattta ttgtattatt
     6601 taataatgtc tattagttct ccattttgta aagttttagg tgtaattact atattgaact
     6661 ctttttgcaa atcttttata atatctaatt ctttttttgt tattattgag attgtgttac
     6721 cacttcgatt tcctctagca gttcgaccac atctatgcag gtattcattt ttactttttg
     6781 gaaaatccaa gttaaaaaca tgacttacat ccacaatatc tagtccacga gcagataaat
     6841 cagttgtaat taaaatcttt gctttaccaa gtttaaattt atttattgca ttctttctat
     6901 cttctttttc catatttcca aaaattccta ttgctttgta attatgataa tttagttttg
     6961 atactaggac ttctatattt ttttcattat ttacaaatac aatagctttt tttggatttg
     7021 tagctgctaa tgcctttcta agaaaagtaa atttatccct tatctcacct aataaatatg
     7081 agtgattgat gttaggattt atttgtgatt tttcctttgt ttttattatt tcaaactcct
     7141 tcataagttt atcacaaatt tttatagtgc tatcagtaag acttgcagag catcctataa
     7201 tttgtctatc tcttaacgtt gttcttatta tgtcttctat acaagttata ttttttccat
     7261 tcaatagatt gtccacttca tctaagataa tagtttttat attatgtgat tttagtttct
     7321 tttgttttat caagtctaaa actcttccac aagagccaat tactatgtgt ggttttactg
     7381 cttttatatt ttttatttgt ttttgtatat tgacctcccc aattaaagct aaagaagtaa
     7441 cggagagttc tgcattttta gccaataatt caacctggtt tgtaatctgc attactaatt
     7501 catgcgtagg tgcaagtata agagcttgtg tttctctttt tgatgtatca attttttcaa
     7561 atataggtaa aaggtatgct agtgtttttc ctgttccagt ctgtgaatta atcaataaat
     7621 ccttattttg aattatattc cctataacaa gtgactgaac ttctgttgga gaagttatgt
     7681 cttgtttttt gagtccatct attagagtgg aacttatttt taattgttca aatgtattca
     7741 tattttactc cttatatttt aataattata gtcaactatt ttttattata cattgtttat
     7801 tcaaatcgaa ctatatattc ttttataatt atgtgtaatt tgaaagtaaa tatcaattat
     7861 atttatacct ttatatttct gatacctttg cattttatag atttctaaaa taaacaaata
     7921 aaagttgtaa ttgtttgata tttgttatat attatatata acaaatatta aaactataat
     7981 atggagggaa attatgttaa aagtaatcaa tgtatctaaa tcatataaaa ataaaaaagt
     8041 agtaagtagt gtctcatttg atgtaaatga tggagagctt tttgggttta taggacataa
     8101 tggagcaggt aaaactacta ctattaaagc aattgttgga atacacgatt ttgaagaggg
     8161 agaggtacta ataaattcta aatctataaa aaaacagcct gttgaatgta aaaaggaaat
     8221 ggcatatata cctgataatc cagatttata cgagtcatta actggtatac aataccttaa
     8281 ttttatagct gacatatttg aagttgccaa agataaaaga gaagagttaa taaaatatta
     8341 ttcaaataag tttgaactca ataaagcatt aggagattta atatcttcat attcacatgg
     8401 tatgaaacaa aaattagcta taatttcggc tttgatacac agcccaaaaa tcttaattct
     8461 tgatgaacct tttgtaggtc ttgacccaaa agcttccttt atattgaagg aaattatgag
     8521 ggagttttgt gacaaaggtg gttgtatatt tttctcaaca cacgttttag aagttgctga
     8581 aaaaatttgt gataagattg ccataataaa agatggaaat ataattgctt atggaactac
     8641 agaagaagtt aagggaagta attcacttga aagtatcttt atggaattaa tagagaaata
     8701 aaatgttttt tatacaatat agtgtctaaa gtgaggaatg ataaaatatg agcaatctaa
     8761 gtatattaat aaaaactaat ataataaatg aatttaaatt aaatgcgtta aaaaaagctg
     8821 atggtagaga aaaacaaaaa ataatgggaa tgatgctttc agttgttata atagcgggtc
     8881 tattaatttt ttatgtaaca agcttaagtc tctctttagt tgatatctta aagcaaataa
     8941 atcaactgga aatgctactg atatttggtt ttggattatc aacattaaca actattgtta
     9001 catcattata taaaacgtca tcatatctat ttcaagcaaa agatttagac cttttgacta
     9061 gtttacctat aaaagaatct actgtactag caagtaagat attaatgttg gttggagcaa
     9121 actatttatt ttcttcaata tgtatgatta tacctgcagt ggtttacttt ttaaattctg
     9181 atgtaagtat aacttatttc ccttatttga tattgctatt tttagcttta ccattattac
     9241 cgatagttgc ttcatcaata atttttctat ttatagggca gatatcatca agaataaaat
     9301 acaaaaattt agtattaatc ataggtagca ttatattgat gttgctttat accctactta
     9361 tgtcaagact taattctatg tcagttgaaa tgctaaagaa tagtgtgtct atttcagaga
     9421 caattagaaa agtatatcct cctatatatt attttgttga tgctcttaaa acaggaagta
     9481 tagtttcatt tttagcattt atagccatct caataatacc attttttatg tttgtaactc
     9541 tatttgcaaa aaaatttaaa gatataaatg ctaagatgag tgaaggatat aagtcaaaaa
     9601 attatgaatt taagggtcaa aaaacttcaa gaccttcaaa agctttatta aaaaaagaat
     9661 ttaagagata tacttcttcg tttatatact ttttaaattc atctgcaggt ttgttatttc
     9721 taatggtagg tacaatttta atattattat ttggagcaga taaagtgggt gagttactga
     9781 aattgaatat agattttaat cttataaaaa tacaattatt gggagtattc ttctttataa
     9841 tagtaatgaa ttgtacaaca tactgttcaa tttctcttga aggaaaaaac ttatggatac
     9901 tgaaatcttc tccaatagat gaaagagaaa tattctggtc aaaaatattg atgaattttt
     9961 tattgaatgc accactatct attttatgtc taatattagt atctataaga ttagactttg
    10021 aaattcagtt tattattgta tcaatctgtt tgattgtaag tttttcaatg tttgtagcat
    10081 tgatgggatt attatcaaat ttattatacc caaaccttga ttggaaaaat gaggtggctg
    10141 tagtaaaaag aagtgcaagt atgatagtta caatgttagt atcattagct tatgtaatta
    10201 tacttggagg tatatatgta ttacttggaa tttcgttttt aaatatatat attattttgg
    10261 caagtatact tactttgata ttagatttta tgatatggag aattatttct actaaaggag
    10321 taagaatatt taaaaattta taggtgtatt tatgtcaaat aaagacttat cttttttagt
    10381 cttgcaaata ataggttaag tagtatacta tgtatagtga tatgaaaaaa tttaggagaa
    10441 aataaatgag aaaattatcc aaaagagaaa aagattttat tcaatatgga tttttattat
    10501 ttttgatatg tattactact tatttagtat taactacttt agatataaaa ctaataccta
    10561 aaattataag gctagtaaat aagacttata tatttcttgg catattgaca attatagtat
    10621 atatgataat tgaatctatt gtaactcatt tgataataaa tgcagttcaa aaaacaaaag
    10681 taaaatttat tggtattaaa atggctacaa tgggatttta ctataactta gttactccat
    10741 ttgcatctgg gagtcaacca atgcaaatat atgcacttac aaaatatgat gtaagcctta
    10801 gtaaatcagt ggctatagtt actaataaaa ctgtagtatt tcaaagtaca gttactgtat
    10861 tttgtggaat tttaattttg ctaaataagt cattactgat ggagcaagta cattctgtga
    10921 aagtcttgat aacttttgga atgattatga atgtaagtat gttgttaggt ggactactaa
    10981 tagtatttag tccaaaacaa gtaaaaatga tagcagaagt tttgattaat tttttatcta
    11041 aatttaaaac atttaaattt ttagattcaa aacgtgatag aataaatcat tatattgatg
    11101 attataatta ttctataaaa atatttatta aaaatgtaag aatcttagtt ttaagtatat
    11161 tacttactgt gttacaatta atcgcatatt tcagtatagt ttattgtgtg tataaagcct
    11221 caaatttaag aggagtttca tatgtacata taatgacatt acaggtcttt ttatatatgg
    11281 ctatatcacc aattccaaca ccaggaaatg ttggagctaa tgaggttaca ttttttacta
    11341 tgttctccaa tataattcca aaagaattat tggggtactc tgtactctta tatagtgggt
    11401 ttgtatacta ttttatatta atagtttcag gtttatttac atttggaacg cattatacat
    11461 taaattcttg gaaaaaggaa aataaagtaa attctaattt agataaggtt attgaaaaat
    11521 taaattaata aaataattag ggaaattcta tatataaata tggtgttgaa taagctatat
    11581 tatattgaga tatttccctt ttttgattaa aattatagaa atttgaaata ataagtaata
    11641 aacagaaaaa gtgatattgt tttgaaagga gaaactatga aacaagataa acaaaatgaa
    11701 aaacaaagtg agatttctaa gataaaacaa agcaatgata ataaacttga aatagctcaa
    11761 gaattacatg taagtaaaaa tatagggaat atgcttggat atagaataga taagaaaaaa
    11821 atgaatagac caagttaatg aaaatgttga aaatggtaca ttttaaaaaa tatatataat
    11881 gaaatgtttt attgttaaat ttaaacttat tttaatgggg gaaaaagtcg aaaaatatct
    11941 aaaaagatat ggatataaaa aatattaata tatatatact ataaacttga caaaatcaac
    12001 cttttaataa ctagcaaaaa atagcaaaat tatataagcg ttatttaatt gtcaaaaatg
    12061 aaagctaaat tgcatgtcct aaaaaaaatg attgataaca tttatttgtg tgctataatt
    12121 atatagtaaa acaataagta tattgcataa tgtatgcaaa tcgggaggag agacggacat
    12181 ggaagcatct atacacacaa ttgccagcaa taatttattg ataatttttg ctattgtaag
    12241 tataactgga ataatttgta gtaagcttag tgaaataata aaggttccag atgtagtgtt
    12301 atatttactt gtaggtattt taataggacc atcattttta aaatttatag atattcgtgg
    12361 atttcaaata gaaaatcaac taatacttac atttggttct gcatttattt tatatttagg
    12421 tggaaaagaa ataagtttaa aagtattgag aaatgtgaag atatctgtat tcttattagc
    12481 tactttagga gttgtgatat ctgcttttat aatgcaacaa gtaataggat ttacttttgg
    12541 gataagtgca atgactgctt tactagcagg aactataata gcatcaacag acccagccac
    12601 tcttgtacct atatttaatc aggtaaagat aaaggatagg gtaaaacaaa ctgtaataag
    12661 tgaatcagct tttaatgatg caacaggagc tatactaaca tcagcagtgt tggctgtaat
    12721 tttgtcaaat aaattttcat taggtgaaaa tatttatgaa ctggggacaa tggttattgt
    12781 cggagtttta gttggtttaa ttacaggaat actgcttttg aaattagtta atgataaacc
    12841 ttatggaata tttaaagatt ttgcaccaat aatatctgtt atttcagttg tagttgcata
    12901 tgaattatct acaaaattag gtggaagtgg atatatgtct tgctttattg taggtataat
    12961 aactggtaac aagaaaaatt ttaaaatttg gttaagtcaa aaatcttatg atgcagattt
    13021 ttatgttgct gaaactttag gtactatttg tcgtatggca atatttataa tattgggtag
    13081 ccaagttaat ttggctcagt taagtaaata ttttatgcca tctgtaatta cagtattagt
    13141 attaatgttt atagcaagac cactttgcgt tttagtttgt acacttgtag acagggaagc
    13201 aaaatggagt aaaagtgaaa tattatttat gatgtgggta agggaaacag gcgtaatacc
    13261 agcagcactt tgtggtatta tttcagcaat gaaagttcct ggatacgaag tgatttcatc
    13321 tgtagtattc atgactatac taattacatt agtaatacaa ggaagtacta caaaattagt
    13381 agctaaaaaa cttggattat tagaagaaga agtgataaat ataagtgaaa aagtatcaac
    13441 tttttaaagt tttgctttaa aatagtacct gttaatggct attttaatat tgtctaatga
    13501 tgattattga aatagtcatt tttttatgta taagtaattt acagaattta catattagac
    13561 atatttatta tcaaaatttg ttgaagctat gatataatta gttgtattta aaataagaat
    13621 agcaaataag ggaggaaatt tatgtttacg gttatggata ttgtacagcc agatacagta
    13681 gaagaagcgt actcaatttt aaataaaaga aaaactaacc aagtaattgg tggaagtgct
    13741 tttttaagaa tggggaaaaa aagaatagga acaggaatag aattatctaa attaaattta
    13801 gattatataa aagagtatga agattacgtt gagattggtt ctatgactac ttttagaaca
    13861 ttagaaacaa gttccataat aaaaaataat tttggaagaa taatagaaga ttcagttaaa
    13921 aatataatag gagttcagtt tagaaatgta gtaacagttg gtgcaacagt attttctaaa
    13981 tatggttttt ctgacttgat agtagcatta ctttcattag atacggaagt agaattatat
    14041 aatattggga gaattagtct tgaagagttt ctaaatagag attatgagaa agatttactt
    14101 attaagatat acataaagaa gacaaataag aatgcttctt ataaatcaat tagaaatgca
    14161 aagagtgatt atccaattct aaatgtatct gtatcaaaac atatggaaca atttaagtta
    14221 tgtgtaggag caagaccgca gaaagctacg attgcaaaac aagctagtga atttctatct
    14281 aacaatgaaa tcaatgaaat taatatagat aaagcagtag aaatagcatc agaagaacta
    14341 acttttggtt ctaacatgag agcatctagg gagtatagaa aagctatgag taaagtactt
    14401 ttaaaaagag ctataatgga ggttatttaa tgttagtaaa gattaatata aatgggaaga
    14461 aaaaaagtgt tgatatagaa ccagaagaat atctggttga tactttaaga aaagttggta
    14521 atttaagtgt gaaaagaggt tgtgacacag gttgttgtgg tttatgtaca gttttaattg
    14581 acaagaaacc tacattatct tgtgcaacac tgacattgag agctttagat aaagaaataa
    14641 ctacaattga aggtttagaa aatgaagtta aagaatttag tgaagttttg gtaaaagaag
    14701 gagctgagca atgtggtttc tgttctcctg gctttatact tactgtactt gcaatgaagg
    14761 atgagcttga aaatcctaca gatgatgaaa taaagcatta tcttacagga aatctatgta
    14821 gatgtacagg gtatatggga cagttaagag ctatcaaaac ttatctggag gtaaaataga
    14881 acatgaatat agttggaaaa ggaatcaaga aaatagatgg aatgagtatc acaacaggaa
    14941 agcctgtata tacagatgat ttatcagcaa aagatgcttt ggtggtaaag atacttagaa
    15001 gtcctcacgc ttatgcaaaa ataaaagata tagatacatc aaaggcagaa atggttgatg
    15061 gtgttgaatg tgttttaaca tataaagatg taccaaaatc tcgatttaca cttgctggtc
    15121 aaacatatcc agagccatct ccatatgata gattaattct tgaagatgtt gtaagatatg
    15181 taggtgacga agtagctata gtggcagcta tagatgaaaa aactgcaatt aaagcaatga
    15241 atcttatcaa agtaaagtat gaagtactta cacctgtttt ggattttgaa gaggcaatag
    15301 gaaataaaac tatggtacat gatgaagata gacattgtaa ctttgatata ggtatgcaaa
    15361 gagaaaaaaa tatagtatcc catcatgatt atacaaaagg tgatgtagaa aaagtattaa
    15421 gtaattgtga tgtagtgatt gaagaaactt attatactca agctcaagca catgcaatga
    15481 tggagacgta tagggcatac aattatttag accatatggg aagattggtt gtagtaagtt
    15541 caactcaaat accatttcat gtaaaaagac atttatctcg tgcactagaa ataccagcta
    15601 gcaaaattag ggttataaaa cctagaattg gtggcggatt tggtggtaaa caaactgcat
    15661 gtgttgagat tttttctgca attgttactt taaaaacagg gaaaccagct aaaataattt
    15721 atgatagaaa agaaactttt aattgttcaa caagtagaca tgcaatgaga ttaaaagtta
    15781 gactaggtgc tacaaatgat ggcataataa aggcaataga tataaaagca ctttctgata
    15841 caggagcata tggagagcat gcttctacaa ctattggttt ggttggagaa aaaactttac
    15901 caatgtataa taaagttgaa gcaacaagat ttattgcaga tgtggtatat actaataaga
    15961 tgccagcagg agcacttaga gggtatggag catcacaagg atgttttgct gttgaatcaa
    16021 ctataaatat gcttgctaat aaattaaaaa tggacccaac agatttaagg ttaaaaaatg
    16081 tggtaacaga aggtgaaact acatttgcat atggtaaaac tttaaatagt gtcgatttaa
    16141 taaaatgtat aaataaaggc aaagaactta ttggatggaa tgaaaaatat ccttgtaaac
    16201 atcttgataa tggaaaaatt agaagtgtgg gtatggcaat tactatgcaa ggttctggta
    16261 tacaaggtct tgataagtct tcagctgaaa taaaattgaa tgactgtgga gattatactc
    16321 ttatgatagg ttctactgat atgggtacag gaagtgatac aattttagct caaatggctg
    16381 ctgaggttct tgatacaagc attgaaaata taacagttat atcagctgat actgacttag
    16441 ttccatatga cccaggttca tatgcttcaa gtactactta tgtaacaggt atggcagtag
    16501 ttaaggcagc tcaagactta agagaaaaaa taatttctgc tggagcaact aaactaggtg
    16561 ttgagaagga tgaagtagaa tttgatggta taaaattaac tagtaaaaaa ggtagcactt
    16621 tattattcga tatagcagtt gattctacaa ttggtccaga tactaatcaa ttaataggtc
    16681 ttgcttctca tggtagccca gtatctcctc caccttttat agcaggtttt gcagaaactg
    16741 aaattgacac agaaactgga aaagttgatt tagtaaatta tgtagctgta gtagattgtg
    16801 gaacggttat aaataaaaat ctagctaaga tacaagttga aggtggaatt gttcaaggga
    16861 ttggaatggc attatttgaa gaagttagat acacagaaaa aggaagcatg gacacaaata
    16921 catttatgca atacaagatt ccagctagat gtgatattgg aaatgttaaa gtagattttg
    16981 ttgaaactta tgaaccaact ggaccatttg gtgctaaatc tgttggagaa gttgttataa
    17041 atacaccttt accagcaata caagaagctg tgtttaatgg aattggtgta agaataaata
    17101 cactccctat tacacctgaa aaagttttta tggaaatgaa taaataatta tttatgaaaa
    17161 tttagactag ttttacatgt taaacaacca gttatgtcat acctgttgaa agatatttaa
    17221 taagtagtta ttataatatt atgaatttta cttataaaat acaaaattaa ataaggggat
    17281 gatgttttgg ctggtaatct aaataatatg agagcagtaa ataattttag aggagataag
    17341 aacattttag aatgtttagt cagctttgag ggtcgttcaa taagtcagag aaaagtaagg
    17401 gtatttttta aagaaaaaca aaatcaaata gaaattgatt ttgcagaaga ggaaatttct
    17461 aaattggttg aaaatgttgt tttaaataca tcatatcaag aaatgttata tgatgaaata
    17521 gagaaacaac tggaaattga ttgtataggt acttggatga tattatctaa attaaaagat
    17581 ggtagtagag ttcactgatt ttaaaataaa ttattagagc ctaattttaa ggctcttttt
    17641 tgctgttttt aattaacaat agaaaattta
//
LOCUS       DAYAOX010000074        17287 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.074, whole genome shotgun sequence.
ACCESSION   DAYAOX010000074 DAYAOX010000000
VERSION     DAYAOX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.074"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(219..1286)
                     /locus_tag="ACGI57_001871"
     CDS             complement(219..1286)
                     /locus_tag="ACGI57_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9716950.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            complement(1298..2986)
                     /locus_tag="ACGI57_001872"
     CDS             complement(1298..2986)
                     /locus_tag="ACGI57_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HFG9716951.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            complement(3004..3999)
                     /locus_tag="ACGI57_001873"
     CDS             complement(3004..3999)
                     /locus_tag="ACGI57_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716952.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            complement(4000..4836)
                     /locus_tag="ACGI57_001874"
     CDS             complement(4000..4836)
                     /locus_tag="ACGI57_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFG9716953.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            complement(4840..6009)
                     /locus_tag="ACGI57_001875"
     CDS             complement(4840..6009)
                     /locus_tag="ACGI57_001875"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFG9716954.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            complement(6010..6723)
                     /locus_tag="ACGI57_001876"
     CDS             complement(6010..6723)
                     /locus_tag="ACGI57_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFG9716955.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            complement(6764..7630)
                     /locus_tag="ACGI57_001877"
     CDS             complement(6764..7630)
                     /locus_tag="ACGI57_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9716956.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQREFKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            complement(7623..8492)
                     /locus_tag="ACGI57_001878"
     CDS             complement(7623..8492)
                     /locus_tag="ACGI57_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFG9716957.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            complement(8482..9597)
                     /locus_tag="ACGI57_001879"
     CDS             complement(8482..9597)
                     /locus_tag="ACGI57_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HFG9716958.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRAAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKIKHPIYEDEEITIGDMLVTECEIVSKASQNK
                     TENSKNNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIKKFEEVSLGDI"
     gene            complement(9600..10175)
                     /gene="phnH"
                     /locus_tag="ACGI57_001880"
     CDS             complement(9600..10175)
                     /gene="phnH"
                     /locus_tag="ACGI57_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFG9716959.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTNLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            complement(10190..10621)
                     /gene="phnG"
                     /locus_tag="ACGI57_001881"
     CDS             complement(10190..10621)
                     /gene="phnG"
                     /locus_tag="ACGI57_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFG9716960.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            complement(10959..11729)
                     /locus_tag="ACGI57_001882"
     CDS             complement(10959..11729)
                     /locus_tag="ACGI57_001882"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFG9716961.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            complement(11759..13696)
                     /gene="metG"
                     /locus_tag="ACGI57_001883"
     CDS             complement(11759..13696)
                     /gene="metG"
                     /locus_tag="ACGI57_001883"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFG9716962.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     misc_binding    complement(13780..14026)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14092..14613)
                     /locus_tag="ACGI57_001884"
     CDS             complement(14092..14613)
                     /locus_tag="ACGI57_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HFG9716963.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     gene            complement(14629..15213)
                     /locus_tag="ACGI57_001885"
     CDS             complement(14629..15213)
                     /locus_tag="ACGI57_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HFG9716964.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            15345..16775
                     /locus_tag="ACGI57_001886"
     CDS             15345..16775
                     /locus_tag="ACGI57_001886"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFG9716965.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            16812..17240
                     /locus_tag="ACGI57_001887"
     CDS             16812..17240
                     /locus_tag="ACGI57_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFG9716966.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
BASE COUNT         5411 a         3066 c         2039 g         6771 t
ORIGIN      
        1 aatgaaatta aaatatacta ttttaaactg tagtatatct atttctaaat taatctctaa
       61 aaataaaaaa tataccattc aaactgaatg gtatacttac tctatacatt tttatagaat
      121 attcactttt cttgacctat attattctca acttatatta taataataaa atctacttaa
      181 aaaaaatttt tctttttact ccatttgtat agttttactt aactctcttt ttctgtctta
      241 ccatcatatt ttgatatcca tttatttagt atatcttctc tattttttgc tgctgatttt
      301 aaatcattat ctatcatttg ctctaatggc tttttaggga atccttctgg tattggattt
      361 cctgtagaaa tagtagtaac tgcatagttt ttagagtatt cgttcattgc atctttactt
      421 atagcccaat ctaaaaatac ttttgatgct tcttttatat tatctttttt cacaagagca
      481 tttgcttcta tatcccatcc agaaccttct tttggaaata ctacttctac tggatagcct
      541 tcctctttta actttatacc tctatatcca aaagatatac caataggata ttctcctgaa
      601 gctgctgatg tagctggagc tgaaccagat tgagtgtata caccaatatt ctcatgtaac
      661 ttatccatat actgccatgc tttttcttcc cccataattt gtataagggc tgatactgtt
      721 aagtaccctg taccagaaga agatggatta ggcatagata taagaccttt atattctggc
      781 tttattaaat cttcgtaaga actaggaact gggatgttct tatcacttaa ttctttagtg
      841 tttactgtta tacctgtcat ccaagcatct atcccaaccc aagaaggaac ttctttatca
      901 tctttaaatg cagactctat ttcttcagaa ccttttggag catatggttc taacatcccc
      961 tggtcatctg caaccaataa gcttgtggct gcaactcccc atacaacatc tgcttgagga
     1021 ttatcctttt ctgctaataa tcttgctgtt atatctcctg ttgatgccct aacaatattt
     1081 aatttaatat ctggatattt ctctttaaat gtagctaaat atggctctat actatcttct
     1141 tcaattgctg tgtaaactgt taaatcccct tttgtcttac tatcagactt tgaatctgaa
     1201 cctgatgaat cagtactaca tcctgttatt acacttaata aaagtgttga taatactcct
     1261 aaagaaacta gcttcttaaa tttcattttt acctccccta tgcaacatct ttttgctgcc
     1321 atttttgtgt gttatttttt aatttctttg ttacaaattc gtacaaaagt cttactatta
     1381 tatttgttaa aagcactaat actgccatag cagctgatgg tcctatatat cctatctcat
     1441 ctaactttag tattgatatt gatgcagtct gagtttgagg tgtatataaa aatattaatg
     1501 ctgaaacagt aaccattgaa tttacaaaat aatatactac tatttcaagt attgatgtta
     1561 tagacattgg aactgttata ttaaaaaata ctttatagaa tggaatattt atggattttg
     1621 caactaattc atactcttta tccaacattt ttaaagatga atttgaggtt ataaaacaaa
     1681 ctgaataaaa atgaactaca tttactataa ctaatattgc tgtacttcca tataaaaaat
     1741 ttaatggatt acttgctgag ttaaaaaaag ttatatatga tatacctaaa actaagccag
     1801 gtaaggccat tggtatcatt gataaaaggt aagctgtttg tcttaattta ggaaacttat
     1861 ccaccttttc tatcatataa gcactaaaaa atacaaatat agttccaaat attgctgtta
     1921 aagttgatat tttcaaactg cttaaatatg tttttattcc acttcccaaa atacctttaa
     1981 atgtatagtg ttccattgta aagcttaaat tatatggcca taactttata actgaaccta
     2041 taactgaaac gaatattaaa ataaaaatta gcaatgttat aaaactacat actattaaaa
     2101 atactctatc tctaaactta ttttccttaa tcttatatgg catagacttt gcagttatgg
     2161 tcataccttg tttcttactt gccatcttat ctaatgtaaa tgctattagt acaggaatta
     2221 acattaccat agaaacagta gctcccattg gtatgttaaa ttgtccaact acttgtttat
     2281 atatgtcagt agccagtaca ttgtagtttc ctccaactac cttagctgct ccaaaatctg
     2341 taaatgcaag tataaaggat acaaagaatg aactaagtaa tgagtacttt ataccaggaa
     2401 ttgttatttt gcaaaatttc ttaaattcac tagcacccaa gctatctgct gcttcataaa
     2461 gtctgtaatc agccatcgaa agtgctacag ataatattaa aaaagcttgt ggaaatgtaa
     2521 atagaatctg agaaataatt attcctaatg gaccatatag tggtacttcc agacccatgg
     2581 ttgtaaagat tcccttattt ccaaaaaggt atataagtga aataccatat aacatagttg
     2641 gtgcaaaaag tggaaacatt cccatagttt taaaaaattc tttaaacttt atgtttgttc
     2701 ttgttattgc gtatgaatat ataaatgcta aacatagtga tattaaagat gagactgtag
     2761 atacaaaaat tgaattttta aatgatgcta taagacttgg attttcaaca tatttactaa
     2821 agtttgaaat tcctataaaa tttccacttg tatccataaa tgcatttttt aataatgata
     2881 ctaatggaaa taatataaat actattagta caattaccga aaaaatcaag aaatattttt
     2941 gtattgtgct atcacttatt cttcttttat tttttgatgt tatcatttat tcacccctag
     3001 ctattaagca catccctctg atttatattg tacaattcct tctttaggta tcttaaagtt
     3061 tattttagaa cctatacaca gtttcatttg ttctgcaact ttggaaacta catctaattt
     3121 catctgctta gaacgatact caactgtaat tctatagtta aatcctctaa attcgatatc
     3181 taaaatttct cccactttta tatccttatc tttagaatct ctattagact ctacttttat
     3241 atcttctggt ctaatagcta tcttattaat accatcatct ataaaattta ttgttcctat
     3301 aaaatcagct acaaaaggtg tttctggatt tttatatacc tcctttggcg ttccaacttg
     3361 tattatttct cctctattca taacaaccat cttatcagct aaacaaagag cttcttcttg
     3421 gtcatgcgtt accattatag ttgtaatgcc tagttttcta tgcaaacttc taatctgctc
     3481 cctaagttta agtcttactt ttgcatctaa tgcagataaa ggttcatcaa gaagtaaaaa
     3541 gtctggctct aaagcaatcg cacgagctaa tgcaaccctt tgttgttgtc cacctgataa
     3601 ttgtgatgga tattttttct catatccact taattctaca acttctagaa cttctttaac
     3661 ttttttatca atattagatt tacttatgtt tttattttct agtccaaagg ctatgttttt
     3721 gtaaacattc atatttggaa ataatgcata tgattgaaaa actataccaa aatttctact
     3781 ttcaggtggt gaattagtca tatccttatc atttattatt atttttccac catgtttatc
     3841 ctcaagccct gcaattattc taagcagtgt actttttcca caaccacttg gacctagtaa
     3901 acaaataaac tctccttttt caatatcaat agatatgtct tttaacacct taacttgtcc
     3961 ataactttta cctacatttt ttacttgtaa aaaagacatt tataataccc ctcctaaagc
     4021 taacctaaac ttaaataaat cttctttatt taagctcttg cttccaagtt cataattatg
     4081 ttgagaataa aatccgtggt aatctgagcc tcctgtgacc actagattat acttcttagc
     4141 ataagaaagc gactcttttt ctagttcttt gttgtgagat ggatgatata cttctattcc
     4201 atctaatcct acttttacca tctcttcaat agcgctaaaa ttatccattt gacccgggtg
     4261 agctaacaca caaattccac cagaattttt tatagctttt attgcatctc tgtaatccac
     4321 atattttaaa gatacatata ccttgccgtt tcctctatga aacaattctt tatatagact
     4381 tgaatatata ttatctgtat atcctttatc tataagagct ttcattatat gttgtttaaa
     4441 tattccagtc tttgatgaat atttctttac atcttccaaa gatattttgt atcctaaatc
     4501 tataatttta tttaccatct taacagatgc tttatgtctg tcacatacca ttggattgca
     4561 caaatttttt atttcttgac tgtttaaatc tatgttatat ccaagaatat gaactctttt
     4621 tctattttta taatcatagg ctgaaatttc tatacctggt attattgtta ttccataatg
     4681 ctctccaagc acaagagctt catccaatcc agataatgtg tcatgatttg ttattgctaa
     4741 gatatttata ttcttttctg ctgctttttt tattatttct tcagtagtaa aattattatc
     4801 tgatatactt gtatgacaat gtaactctgt tagcaatctt tatacctccc ttttataaac
     4861 tgtttttcca tttacaatag tatttacaac ctttggatat tcactatcaa tatctactaa
     4921 aactaaatct gcttttttat tttcttttat actacctctt tcatcatata taccaactgc
     4981 tttagcagga tttgaggtaa ataaactgac tgctttattt aaattattta ttctttctgc
     5041 aactatacac atagacttta tcatagctga tggaagataa tccgaacaca caatgtcaca
     5101 acaatcttcc aatatagcat ccattgcttt taaattgtta ttatgagatt tacctcttac
     5161 aatattcggt gcacctaaac aagttagaac acccaatttc tttgaatgtt ttgcaacttc
     5221 aagagttata ggaaattcag atacatttat gcctatattt tttatgaaat ccattttttc
     5281 taatgtatca tcatcatgtg atgcaacatt tatattttta gttttggcta tacctataat
     5341 attagctatt ttattccaat ctaaattatc atgcagattt actaaatcat ctaaccatgt
     5401 atctacataa ttttctgtaa ctccccacac tttagtagca tactctttat aaaatgttgg
     5461 atttgtatac tgaccttgtc ctggagaatg gtccattatt gataagtagt ctattttatt
     5521 ttcatctaat agttctaaaa ccttttctaa accttcataa tacaacactt catatcttaa
     5581 atgaacttta tgatttatgc tcttactatc tatattttta tatgaatcaa tatttttaat
     5641 catctttaaa gagttatcaa tacttctaac tcctactcca tcccctaaag atatagaatg
     5701 ataaactgta gttattccat ttgcaggtag ttttttatct agttcataaa atgccatatt
     5761 aaatggtaat aatgtacttg gtctcggctc tatttccttt tcaatggcat cactgtgaat
     5821 atctataata ccaggtatta catataagtt ttttgcatct attatttcat ctatctcatt
     5881 aaagtcaaat gcgtaatcaa tcttatcatt tgatactttt tttattgtat ctccctctat
     5941 gattaaatga ccattttcta aaatcatatc ttctaataca atatttgcat tatgaattag
     6001 ttttatcatc taggctaccc cactttcact tatagtccct tttgtcatag taaaggtctt
     6061 atctataaca gtttccataa actctatatc atgaaaaatt cctatcattg tagttccttg
     6121 acttttaagc tccaacattt tttcttttac atatatttta gattgattat caagtgatgc
     6181 agttggttca tcaagtaaca aaagtctagg ctttttaacc atagcttgag ctaaatttaa
     6241 tcttaatttt tctccaccag aaaaattatt tggatacgca tcccataaat tttcctttat
     6301 ttcaaattgg ttaagtatat cttttgccat atcatacgaa caatcctttt caaacccact
     6361 ctcaattaaa gaatgtacta ccagttcaat tgcagttatt cgtggaagtg tctttaaaaa
     6421 ctgtgataca tatcctattt ctttctttct aagatttatc atttctcggt ctccaatttt
     6481 agccaggtct acattaccat atatttctga attaaaaatt atttctccag ttgttggtat
     6541 atatgtttta tagatacttt ttaatatggt agacttacct gctccagatt tacctattat
     6601 tcctaaaaac tcaccttttg aaacttcaaa acttatatta ctacatgccg ttatagtctt
     6661 gttcaatgca tgtaactcaa agtctttact aaaattattt acttcaagta tagtttcttt
     6721 cattttatta aaaaccccca ttttgcttat ttacaaacga tatttatagc aatgaattta
     6781 taagttgctg agtatatggg tgttgtgggt cctccattat ttggtctgta agaccagact
     6841 caattacact tccatttttc attacaattg tcctatcagc aagcatcttt ataacatcaa
     6901 agtcatggga tacaactatc atagaaatct taaattctct ttggatttct cttattaaat
     6961 ccaatacttc cgcttgcaca gaaacatcta aacctgttgt aacctcatct aatagcaata
     7021 ttggagggtt atttgaaaga gcttttgcta tttgaactcg ttgttgcatt cccccactaa
     7081 agtattttgg tttatggtct attctttcta caggtatatt agtttttatt aaaagttctt
     7141 tgcctctatc tcttattttt tcaacattat ataaatttgc catcaataat ttttctgcta
     7201 tattaccacc actactaaag tctaaattta aaccaaggtg gggattttga tacacaatac
     7261 ccataaaatg attctttatg tatctctttt tttggtctga aagctctagt atatttgtgt
     7321 ctttattgta atgggatata tatacttctc cactatttgc atgttcttgg aaaaacagta
     7381 tttttaataa agtgcttttt cctgaaccag attcacctac aattccaagg acttctcctt
     7441 cataaagttc taaatttaca ttattacatg caactataga accacaatac ttacatatat
     7501 tattgctttc atatcctgtg ctttcaaaac acttttcaca cccatctcca taaataagat
     7561 tgatatttct tcctattaat acttgcttgt ctatattttc taatctttta gagtcattta
     7621 ttttactcat tccaagtacc tcctattgtc acatctatac tgttattttt taatctaact
     7681 ttttcacaat aacttgtatc tgagcaacta tagtactttt cattagtgtc actgtcatat
     7741 acttcatcta aaaatgtatt tgtactgtta cataaactac agtattttcc ttcaaacttt
     7801 tcctcttcaa acttataatc ctcaaactct aaaggttcta cctttgtata tggaggaata
     7861 gcataaattc ttttttctct acctgcacca aacaagaata aattgtctga catatttaat
     7921 ttttttatat caaatctagg tataggtgat ggattcatta aatatctatc atttacttta
     7981 catggatatt caactgctat agatatatca ttaaatctaa ctatatcttc ataaagatat
     8041 acccacatcc tactataatc ttcttctgaa tgcattcttc ttgtttctac ttcactaggt
     8101 tctactattc taagggtttc tggtattggt acttgaaata ttagtatttg attagattta
     8161 agtttttctt ctggtattct atgtctcgtt tgaatcaatg tagcttctaa tgtatctttt
     8221 gtggtttcta cattacttac acttgcaata aagctcctta tattacaagc atttgtagaa
     8281 tcatcacttc cttggtcaat tacttttact acatcatttt taccaattaa tgatagtgtt
     8341 aattgtaaac ctccagtacc ccatccctta gctataggca actctcttga accaaaggga
     8401 acttgataac caggtattga tattgctttt aatatctttc ttcttatttc tttttttgtg
     8461 ttttcgtcta aaaaaccata gttatatatc acctaaactt acctcctcaa acttttttat
     8521 tctttctaca tccgctttaa aatctacata gtgaggaagc ttaaagtgag atataaatcc
     8581 taatgaatct attccatcta tatgaagcaa tacaaattct tcgtcttgtg ctagtttttc
     8641 actatttggc tccaaagatt tgtctagtat agccatagat atagccttat tctcattgtt
     8701 ccccatacaa agaccatatc ctaaagacat tttattttta tcttccgaat tatttttaga
     8761 attttcagtc ttattttgcg atgcttttga aactatttca cattcagtaa ccaacatatc
     8821 acctattgtt atttcttcat cttcatagat gggatgtttt atttttataa acacattgcc
     8881 agttctaagt tcacttatat atggatgttc atcaccaaag cctcttacaa tagcataagc
     8941 aaaacctgat actactccgc tttcacctct agttaatgcc tgtaactttg ctgccctaga
     9001 aactggaaat cttaaactat ttcttgtaat atcatcaata acttcttcca atttgttttc
     9061 acaaacttct tcttgtaata aattttcttt tctaaatata ttagatattt ttgtaaattc
     9121 agggatttca tctactgttt ctaattcatt tataaaacta tctctaaaat gtctattctt
     9181 tttttcatct tcttctaata attctacatt caatattcta ttttcatagt ctctagttgc
     9241 cccaagatac tgacctcctg gtatatcttt aaaagtagct gatattcttc tttttattct
     9301 catttcttct gtatttatgg gttctgaata tgtaattcta ggcattgttg accttaaagc
     9361 tcttacatag aaagaggctt ctaatatatc accttgagct tgttttatag ccaatgctga
     9421 tagttccttg ttgtataaac ttccttcaga cattactttg tctacagcat aagacatttg
     9481 ttcctcaatc tgctttacat ctataggcaa gctatctcct tttaccctat aaaactcaag
     9541 tatattttta gcctgtaata tggcttcttc tccaccttta actgctacat aagccatttc
     9601 tataatacct cccaacttaa ttttgatgac cttggtataa atgcaatttc tcctttttta
     9661 tctactaaaa tcaaatctat tcctattgga taatctttat ttatactaga taatctttct
     9721 ataaattctt ctctaattcc atttatatat atactatttt tagattttat accaggacca
     9781 ctcaaatcaa gctttatata cttagtttga gagtacgttg ataaatcatt tacttgatgt
     9841 actatagttg cactttcatc aggatattca aaagaaccta ttttaatctg taatatatcc
     9901 acttctgaat taatatctaa aaatatataa tctgattttc tataatctat ttgatttgac
     9961 attgtaagtg ctcttatatt ttttatatct ttttgagtat aattttctat atagaaggta
    10021 acttcttggt ctaataaagt gattgctata ccaattgctc cattcaatag atttgtatta
    10081 ttttctatat ttatattatc tatattattt aatttcccag gaaatgaagc actatctagc
    10141 atctttctat atatttcttg tgtataattt accatgattc tcctcctatt taatccatag
    10201 cattgaattg aactttcgtt ttattaatca agctaaattc atcaataatc tcttgttcat
    10261 accttaagtt ctcttcatat acacttttat ttatgtaatt ctttaactca tcaaatttac
    10321 tgttattgct atgtaaaact gcatctataa ctgctaaata aatggctttc ttattattgt
    10381 tttctgaaac tattccgtaa cccaagcttt catctacctt tacactacat tcagtaacta
    10441 atatctctcc taaattaaat gttgtgtttt ttacactttc attagctctt accattactg
    10501 tagccaagtt aggttcttta attatagaaa tacttgatgt atcttttatc attgagtata
    10561 tcttttctag tttctctaag ttcatattta tcaatatctt agtactatca actgtataca
    10621 aaattcaacc tccttaatct acctttcgtt tacttctctt taataatata ataaacaact
    10681 taagttaagt gaatattaga gttgagttaa ttctaatgaa tctcttgtaa attctttttt
    10741 aacaatgtta agaaaatata ttttaaaata tgaattaaat aaataaaaac aaactatctc
    10801 aaaatatgat aaacttcttt ttcattgttg aaatgatgaa aatatatttg atttcataca
    10861 aaaaagagtg cctcatgaac taaaaaactc attttgaggc actctcatcc tatacttata
    10921 atgacttatt atatatatca ttataagatt taatttgtct attttatatt aaaaaatttc
    10981 tttgcatttt cttttgtaac tctacatact tcttcataag atatcccttt ttcctgggct
    11041 atcttatcag caacaaaaga tacttgggaa ggattgtttc tctttcctct atgtggctct
    11101 ggtgacatat atggtgaatc agtctctatg aataatcttt ctagaggtat ttctcttact
    11161 acttctctag tttttttatt attcttaaaa gtaaccgtgc ctggaattga tatataacaa
    11221 cccatcttaa catattctct agctaactct acattcccac tataacaatg aagtacacat
    11281 cctatttcag gatttttagt attctttatt atctcaaaag tatctccatg tgcatctcta
    11341 tcatgtatta ttattggaag ttttagctca ttagctaatt caatttgctt cttaaaccac
    11401 tctttttgtt cttctcttgg agaataatca taataataat ctaatccaat ctctccaata
    11461 gctacaactt tttcattctc agttgcaagt tttcttagag tttctattgc tgtatcatct
    11521 agttttgata catcatgtgg atgtacccca actgctgaat atataaagtc gtattttttc
    11581 gcaagttcaa ttgctgttat cgacgtctct atatctgctc ctggatttac aaccaaatct
    11641 acacctttat cttttaatga accaataagt tcatctctat cttcatcaaa actttcatca
    11701 tttaaatgtg catgtgagtc aaataacatc tttaatctcc ttactattta attacctact
    11761 aacgaacctc gcatccatct ttagcccctt gtgtaaatgg taaaacatat gggtctccat
    11821 catcacctgc tgctaatatc ataccttgag attctacacc tcttaattta actggtttta
    11881 agttacatac cactgtaacc tcttttccta ctaaatcctc tggcttatag tattcagcta
    11941 ttcctgatac tatttgtctt gtttctggtc caatcttaac ttgtgatact aataatttat
    12001 ttgcttttgg atgtttttca cagcttatta ttttccctac tctaagctct attttgtcta
    12061 aatcatcaat agtaatttct tctttatgct ctaaaggttt ttcttctact ggaggtttcc
    12121 cagagaacat tttagttaat tcttctatct ctttttctac atctaatctt gggaataaag
    12181 gttcaccttt aaatactttt acattttctc caataagacc aaatgttttt gtactttccc
    12241 atgtagttat atcatcttga ccttttattc ctatatgttc atatatttta ttagcagtct
    12301 cattcattat aggatttatt agtgttgcga ttattcttat agattcacat aaattatata
    12361 aaactgtgtc taattcatct ttcttcgttt catctttagc tagtgcccaa ggcatagttt
    12421 catctatata tttgttagtt cttctaacta atttccatac actctctaat gcttcatgga
    12481 attgcatttt atccatttca gcttcaaagt tttctctagt tgaaacagct tgttctttta
    12541 agtcagcatc aaaatcagtt gaaacttttg ctgttggtat tataccatta ttgtattttt
    12601 caaccatagc tacagttcta cttattaagt ttcctaaatc atttgctaaa tcataattta
    12661 ttctcgttac aaaatttcta tgagtataac ttccatcttg cccaaatgca aactctctta
    12721 ataagaagta cttaagtgtg tctattccat atctttctat tataggttct ggataaacca
    12781 cattaccctt tgatttactc attttatcat cagcaaatag tatccatcca tgtccaaata
    12841 cttgttttgg aacttctaga cctaaagcca ttaaaagcgc tggccatatt attgtatgga
    12901 atctcactat ttctttacct actatttgaa cattggcagg ccaatatttt ttaaattctt
    12961 catcattatc agtcatataa cctaatgctg ttatatagtt acataaagca tctacccaca
    13021 cataaattac atgtttttca tcaaaaggaa cttttatacc ccagtcaaaa gtcgttctag
    13081 ttacgcttaa atcttctaat cctttgtcta aaaagtttgc caccatttca ttttttctag
    13141 cagcaggaaa acagaaacta tcatctttaa ataactcttt taatctgtct tcatatttag
    13201 ataatctaaa gaaataagat tcttctttta ctaaatatgt ttctcttcca cagtcagggc
    13261 atttatttcc ttctagtaat tgagattctg tccagaaact ctcacaagga gtacagtatc
    13321 taccttcata ttcaccttta tatatatcac cttgctcata tagtttagta aatatctttt
    13381 gtattatgtc tgtatgtctt ttttctgtag ttcttataaa atcatcataa gatatatcca
    13441 tagtattcca taaggccttt atatctttta tcattccatc aagatactct atctctgaca
    13501 ttccttgttc tatggctttc ttttgaattt tttcaccatg ctcatctgtt cctgttaaga
    13561 actttacatc atatccacag aaacgcttga atctagctat tgtatctgca gctactgttg
    13621 aataagtatg tcctatgtgt aatcctccac ttggatagta tattggtgtt gttacataaa
    13681 aactcggttt gctcatgtta tttacctcct aattttttat cgttcgtaat catatgattc
    13741 tttcatttaa ataataaaaa atcgcctcct atgagttaac ataggggcga gattattcac
    13801 ggtaccaccc taattcatct ataatagtta tgtttcacat gaaatgtaga aacattttat
    13861 agatacctta gtagactata acgtgtccaa ccgttttacc ctagcacact tgctcagata
    13921 aaaagctcca agaccatctt caataaagct tttgacacta gctttcacct aacctagctc
    13981 tctgatagaa aaaaccttta tttactcttc ttttcatagc ttgtaatttg tattactatt
    14041 tgtttctata taatcactat attataaaat ttttcccata ttgtcaacaa attatacaaa
    14101 tattacatag caaataaaaa cagctgctat gacacctgca acatggctaa tcatagcaca
    14161 tgataatgta tgtctagtat tttttatctt cagacttcca tagtatattg ccattgtgta
    14221 aaaaatagtc tcagaagaac ctacaatcgt agcacccatt ttttctacta aacttcctat
    14281 tccaactcta tttgcaagtt cactgtacat ccctaaagca ccacttccag ataaaggttt
    14341 tacacttatt agacctatta attcttttgg tatactcatc atatttgcta ttggtgtaaa
    14401 tagccattct aacatatcaa gacctttacc agttttgaat attcctattg ccaaaaatat
    14461 acctattata tatggtagga tatcccatgc agcttttaat ccatcaattg ctccttttac
    14521 aaaactatca tacacatcta tttttttata tttaccataa attactatgt acagaattat
    14581 tattggtata agtaagtttg aaaatatctc catgtatcta catccccttt atctatatct
    14641 tctttgaaaa aatttacatg ttattattgc tactgctgta gatattatgg ttgcaaataa
    14701 agtcgtaaaa attatttcac ttggattttg agcccccata tccatcctta gtttgattat
    14761 ggtaaatgga attatttgta tagatgatat attgattacc ataaacataa tcatatcatt
    14821 agtggctgta tctttcttgt tattcaaagt ttgaagctca ctcattgctt ttaaaccaaa
    14881 tgctgttgca ccatttccag cacccaacat atttaaagca atattcataa ctatataaga
    14941 cattgcttta tgaccttttg gaatagaagg aaataatctt ctcattatag gatttagttt
    15001 ttgacctatc ttttcaatta aacctgattc ttttgcgata ttcattatcc ccatccagag
    15061 tgccatgata ccagctagac ttattgcaaa ctctatacct tttgatgttt cacttaatat
    15121 aactttattt aattcaccca tatttttaga aatcaagctt cctattattc ctatcgatac
    15181 catataaaac catatttttc ctattataat catcctcctc tttaaataat tttatgatag
    15241 ttaggtaata ctttatactt gaaagttgta ctatattatg ttttagtata agtgtgggag
    15301 tttttacaat ttttaatatt taataataat taaggagaga aataatgaga aatctaacac
    15361 ttcttactga tttatatcag cttactatgt taaatggcta ttttgaaaag aatattcatg
    15421 aagacattgt tgtatttgat atgtttttta gaaaaaacgc ttgtgatgga gggtatacta
    15481 tagtttgtgg tattgatcag gttgttgaat acatagataa tcttcatttt tcagatgaag
    15541 atttagagta cttaaaaaac ttaaatttat tttctgacaa gttcttaaaa tttttaaaag
    15601 aatttaaatt cacaggagat atatatgctg ttgaggaagg aactatcatg ttccctaatg
    15661 agccattaat tactgttaag gcacctttat atcaagctca acttattgaa acagcattgt
    15721 taacaatagt taattttcag tctcttattg ctactaaagc ctctagagtt tgctttgcag
    15781 ctcaaggaga ccctgtattt gaatttggat tacgtcgtgc acaaggtcca gatgcaggga
    15841 tatatggggc tagggctgct gtagttggtg gttgtgctgg aactgcaaac gtactagcag
    15901 ggaaaatgtt tgatatacct ataattggta cacaagctca cagttgggta caaaaatttg
    15961 ataatgaact tgaagcattc caagcatatg ctgatgttta tccagataaa tgcctactac
    16021 ttgtagatac ttatgatgta ttaaacagtg gagttcctaa tgcaataaaa gtattcaaaa
    16081 atatttctga aaaaggatac aaacctatgg gtataagact tgactctgga gatttagctt
    16141 atttatctaa agaagctaaa aaacaattag ataatgctgg cttttcagat atcagcatca
    16201 ctgcatctaa tgacctagac gaatatacca taacatcact taaagcagaa ggagctacta
    16261 taaattcatg gggagtaggt acaaagctca ttacatcttt cgattcacct tctcttggag
    16321 gagtttataa attagctgca tcttgtgaaa aaggagttct tgaacctaag ataaagattt
    16381 ctgagaatcc agaaaaaata aataatcctg gttacaagaa agttataaga atatataacg
    16441 aagacaataa ggcagaggca gatttgatta tgttacatga tgaagttata gatgagtcta
    16501 aacctttaga aatcttccat cctacttata catggaaaac gaaggtattt acaaattaca
    16561 aagttaaaga gcttcttaaa ccattgtata taaaaggcag atgcaaatat aataaaaaag
    16621 ctgttcttga aataaaaaat catgtacaat atgaattaag tactatctgg gaacaataca
    16681 aaagactttc aaaaccacac atttataagg ttgatttatc tagaaatctt tggtatttaa
    16741 aaacacaaat gatagattct aaaaaagttt tatagcatta attattttta tttaaggggg
    16801 actgtttatt tatggatgtt acagattaca gaatcataga aattttacag gatgatggga
    16861 gaatttctat gaaggactta ggaaaaatag ttggtttaac ttctcctgca gtttcagaaa
    16921 gagtcaaaag attagaagag tctggtgtta tagaaggata taaagctatt gtcaacccag
    16981 attcattagg cagagttata aaggcattta ttcatatttc tcttccaagc aatggatata
    17041 cagaatttat tgagtcagct gcaaaggacc ctaggattgt agaatgtcac catataacag
    17101 gtgatgattg cttactttta aaagtaattg taaaagatat gtacgagtta gaaaatgtga
    17161 ttgatactat aaagaaaatt ggttctacta agacctctgt tatactatca acgccaatac
    17221 aagcaaaatc aatattgtaa tataaattaa atactaatgg tttattaatt aatagaccat
    17281 taagtat
//
LOCUS       DAYAOX010000075        17273 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.075, whole genome shotgun sequence.
ACCESSION   DAYAOX010000075 DAYAOX010000000
VERSION     DAYAOX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.075"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(349..1599)
                     /locus_tag="ACGI57_001888"
     CDS             complement(349..1599)
                     /locus_tag="ACGI57_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HFG9716967.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1631..2572)
                     /locus_tag="ACGI57_001889"
     CDS             complement(1631..2572)
                     /locus_tag="ACGI57_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFG9716968.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2812..3888)
                     /locus_tag="ACGI57_001890"
     CDS             complement(2812..3888)
                     /locus_tag="ACGI57_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HFG9716969.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4261..4776)
                     /locus_tag="ACGI57_001891"
     CDS             complement(4261..4776)
                     /locus_tag="ACGI57_001891"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HFG9716970.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(5139..6209)
                     /gene="leuB"
                     /locus_tag="ACGI57_001892"
     CDS             complement(5139..6209)
                     /gene="leuB"
                     /locus_tag="ACGI57_001892"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFG9716971.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(6216..6707)
                     /gene="leuD"
                     /locus_tag="ACGI57_001893"
     CDS             complement(6216..6707)
                     /gene="leuD"
                     /locus_tag="ACGI57_001893"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFG9716972.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6722..7999)
                     /gene="leuC"
                     /locus_tag="ACGI57_001894"
     CDS             complement(6722..7999)
                     /gene="leuC"
                     /locus_tag="ACGI57_001894"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFG9716973.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7956..9698)
                     /gene="leuA"
                     /locus_tag="ACGI57_001895"
     CDS             complement(7956..9698)
                     /gene="leuA"
                     /locus_tag="ACGI57_001895"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFG9716974.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTTQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9822..10068)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10216..10662)
                     /locus_tag="ACGI57_001896"
     CDS             complement(10216..10662)
                     /locus_tag="ACGI57_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716975.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(10683..11402)
                     /locus_tag="ACGI57_001897"
     CDS             complement(10683..11402)
                     /locus_tag="ACGI57_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HFG9716976.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(11520..12794)
                     /locus_tag="ACGI57_001898"
     CDS             complement(11520..12794)
                     /locus_tag="ACGI57_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HFG9716977.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNVKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(12994..14361)
                     /locus_tag="ACGI57_001899"
     CDS             complement(12994..14361)
                     /locus_tag="ACGI57_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9716978.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(14611..15048)
                     /locus_tag="ACGI57_001900"
     CDS             complement(14611..15048)
                     /locus_tag="ACGI57_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HFG9716979.1"
                     /translation="MGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVYVFSVIGSTL
                     VSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGIILFVGIPLPT
                     TGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(15080..15709)
                     /locus_tag="ACGI57_001901"
     CDS             complement(15080..15709)
                     /locus_tag="ACGI57_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HFG9716980.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(15743..16630)
                     /locus_tag="ACGI57_001902"
     CDS             complement(15743..16630)
                     /locus_tag="ACGI57_001902"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HFG9716981.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(16659..>17273)
                     /locus_tag="ACGI57_001903"
     CDS             complement(16659..>17273)
                     /locus_tag="ACGI57_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6080 domain-containing protein"
                     /protein_id="HFG9716982.1"
                     /translation="NMIFEFKLKIGKMLKHLITFILMAYICVVSTKSYESFMNLGNVI
                     QYDTKFISGEPILEKIAMIIERLLYSVFYFPGASIKKGLFLQNGEVATIPVILTLLAL
                     CFCVLSVIENSEKRVPKLCMGIIIFNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYF
                     TKALRKNEKNMYNIFLSLLLVTIVISNVNGFIEILNIGIKSYPV"
BASE COUNT         5517 a         3187 c         1765 g         6804 t
ORIGIN      
        1 tttactattt tatttttata aatataataa ttatatttat aaaaataaaa tataaatttc
       61 aaaatcactt tttttattaa cttttttata attataataa aaatatataa cagtactatt
      121 ataaatacac atttaaaacc ttataaatat acaaatacaa ttatatgaat tttcatcctc
      181 aattatagca tagaatatga ctatctatat cctaaaatta tccatttcta aaaaaaaggt
      241 aactattatt aagaatatca acttatagaa tttatattcc acatacgatt ttcaatcaaa
      301 aaaacaaggg ctaaaaagtg aattgtgcac tttttagccc tttattaatt attcatttat
      361 ttcatgtaag aatagaccac atcttacttt aggttcaaac catgttgact tagctggcat
      421 tatcttattt gcatctgcta ctcttattaa atcttcaata ctagtaggat acattgcaaa
      481 tgctactttc atatcttcat taactctttt ttctaattct tcaagacctc taattccacc
      541 gataaaatca attcttttat ctactctagg attatcaatt cctaaaatag catcaataac
      601 atagtcttgt aatatagaaa tgtctaatga gtcaacaggg tcttctattt ctaataaatt
      661 tttatttgct ttcattttat accacttatc acctaggtac attccgaaag ttcctttttt
      721 gtcaggttta tatttttctt ttccttctat ttcttctagt tcaaaatttt cttttatttt
      781 atttataaat tcttcctcgc ttaaaccatt taagtctttg ataaccctat tataatccaa
      841 aaccattaaa ttctcatctg gtgcaatcat tgctatgaag taattaaatt cttcttttcc
      901 agtataattt ggatttttag ctctcatttc tttggctaca ttttctgctg aagctgtcct
      961 atgatgacca tctgcaatgt atagataatc taatttttca aatttatcta ctaggttatc
     1021 tattatatca tcattatcaa tcttccatac tgtatgctct atcttgtcat ctgttatgaa
     1081 atcatataaa ggttcattat tatccataaa atcattgatt atatcatcta tcaccctttg
     1141 atgtttgtaa gttacaagta tagttcccgt attagcatca cagtatttta tgtgtttagt
     1201 tctatcaatt tccttttctg gtcgagtgta ctcatgtttt tttattgttc cattcaagct
     1261 ttcatcaact gatatacatg ctactatacc aacttgagct ctaccatcca tgatttgttt
     1321 atatatatat atacactctt tgtcatcttt tactagaatt tcttgttttc gaaactcatc
     1381 taaattttct cgcgctttta aataaacttt ttcatcatat aaatctatat tttcatctaa
     1441 atcaatttct gatttatcta tatgcaaaaa tgaatatgga ttcccttctg caattcttct
     1501 agcttcttta ctattcataa catcgtaagg aagtgctgct actttatcta ctatatattt
     1561 atttggtcta atcgccttaa atggtttaat cgttgccatt actaatttcc tcctaattat
     1621 acaaaatatt ttataatgct acccctatta gattgttgaa gtcaagaaag aaatcttgaa
     1681 tcacatttac tatttcgtct cctattcttt cttgagcttc tactgtagat gccccaatat
     1741 gaggtgtagg agaaactctt ggatgattta ctaagtctaa atttaatgta ggttcttgtt
     1801 cataaacatc tatagcagct gctgataatt taccttcatc taatgctttt agtaaatctt
     1861 tttcatcaac taaaccgcct cttgcacagt ttattaaata agcccctttc ttcatcttat
     1921 ttatttcttt ttcagttatt actgccccct tattcttatc aaatggtatg tgtaaagata
     1981 agaaatctgc attttctaaa acttcttcca tatcacagaa tttataattg ttaaagcctt
     2041 gcgcctcacc cattatgtca gtatatataa cattcatacc taataattct gctctctttg
     2101 caacttcttt tgcaattctt ccaaaaccta taagtccaag tgtctttcca ttaatttctg
     2161 tacctttata ctttttcttc tcccattttc catctctcat agttacattt gcagtattta
     2221 tgaatcttgc aagaacaaat aattgaccta ttgtaagctc tgcaacagat atactgcttg
     2281 catttggagt attcataact tttatgccat tagcttgagc atatttaaca tctatattat
     2341 ccagacctac tccacctctt actatcaact tcaacttttt accctctaat gctttatcta
     2401 ttacatcttt agtaacttta gtagctgaac gtactacaag tacatcaaca tctttaagtt
     2461 tatctcctaa aacatctttt tcataaaact gctctatcac attaaaatct aactctctca
     2521 attttctcgc tgcttctttc tctattccat cagtaactaa tatattatac ataacaaact
     2581 cctccaattt ttattcactt aatttttcca atttttattt tttaaagtat tttcatcaat
     2641 aaaaaacttt acttaattta actttctgtt tatataattt tcaaaatatt tttttattaa
     2701 agtcatatca aatttttcta ttgtttgtta aaaatttaat atgtaatttt tataaaatat
     2761 tatttattat atattctaaa tttatcttaa aacatacata tttttacaat attatttatt
     2821 aaaacctaat atatcatcaa tattatctaa taattcttgt acatcttcga cagtatagtc
     2881 acccatatgt gaaattctaa aagttttttc ttttaaatct ccatagccat ttgctatttg
     2941 gaatcctcgt tcttgtaatt ttgaatttaa atttgataca ctaatacctt gagtattttc
     3001 tataacagta agtgtattgg ataagtgatt ttcattggta aaaatttgga agtgtttttt
     3061 agcccagttt ctaactaagt ttgccatgtc ttcatgtctg ttgaatctgt tatctaggcc
     3121 ttcatctaat atattatcta attggaaatc taaagcgaac atgtgagaaa gagatggtgt
     3181 tgaagggtat tggtagtttt tcttgattaa gtattcatac attgctaaca aatcaagata
     3241 tactcctcta aaaggtactt gcttagctct atcgattgct ttttgagaaa atgtgcaaat
     3301 agacattcct ggtggtaatc ccagtgcttt ttgtactgat gttatacaaa tatctattcc
     3361 aatcttatca acctcgactt ttattccacc ggctgaactt acagtatcta cacagtagat
     3421 tacatcctca tactttttaa caacttctcc aatttcttct attggatttc ttattccagt
     3481 tgaagtttca ttgtgagtta cagttattaa atcgtattct ccggtagaaa gcaccttatc
     3541 aaccatttct ggcgttgttg cctctcctaa ttcaacttta aatatatccg ctgggacatt
     3601 attagccact gccattttgt accatctatc accaaaggat ccacaggaaa atactgctgc
     3661 ctttttggat gtacaggaac gaatggagcc ttccattaaa ccagttccgg atgacgtgga
     3721 taaaagtatc gtgttgtttg tatagaatag tttttgcatc ttttcactta tactcttttg
     3781 gagcatagat gcatcttttc ctctatgtcc tatcattggt gtagccatct tttgcaagac
     3841 ttcttcttta acatcaatag gaccaggaat aaataatttc ttactcatta taattccccc
     3901 ctttttagtt tgtaatatgg atagcgtttt tactgtcctt atttttaata actataatat
     3961 caagctgaat taactatgtc aacataaaat ttatttttta tatataattt attttatata
     4021 ttttatgaaa acattcattt tctacctttt cataagtctg aattttctga attattccct
     4081 caaaaaaaat atacttttta tctatataaa ttaatttttt tagtatgtaa attttacgct
     4141 attacgataa atctttttag tataactaat ttatttttat cttgtatttt tattgtattt
     4201 ttgaatttta ttttttctaa atataaaatt tatgttttaa aatataaaat ttttatttaa
     4261 tcaaagttca atatattcca ctttttcttt cgattcttca aaaaaccatt cttcattaga
     4321 aaaatcacct tctgcaattt gtccaatagg cattttcata tactttccag ttgctgtcac
     4381 tgctatatct ccatttggta gaattatttc tcctgtacct tcaaataatt ttctgttttc
     4441 tttagttatt cttccaacaa cttttatagt agcatctgtt ggtactggtt tcttatattt
     4501 caactctaaa gatactgtta ctccccatat ttggtcatca tttatactta cagctcttcc
     4561 aatagtctca tccaatattg cagcagacat acctccatga actctcccag gataactttg
     4621 atgccaatcc tttgtattac aaattgatac aagctctcca ttttcaagtt cataaaaatc
     4681 tgctttaagt cctagctcat tttgtgttcc acaaactaaa caagatttac tactattttg
     4741 cttcttcaaa actttatact ttgccaaaaa atccatctcc taaccgacat tagtagtcgc
     4801 ttaatattaa ataattctaa aatatattat ataatattaa taaaatccct gcaaacttct
     4861 aataataaaa ataggctact tcaatatgat aagtttaatt taaagtatta ttttccaaaa
     4921 aataacttta tcaaaataga aactcaaagt agatataaac ctctttttcg ttattaaaat
     4981 agtaaaaata tatttgactt aatataaaaa agagtgtctc attaactaat aaagtttatt
     5041 ttgagacacc cctcataaaa caaattatac tacaatagat ttttactaat tctacaagtt
     5101 atatatttaa attatattat tcaaattaaa ctataattct aaatttcatt tactattaat
     5161 tcgcccattt cccttgttcc tacaacattt cccacaccat tatagatatc aatagttcta
     5221 taacctttat tgagtacact ttgtacagca tcttcaatac acttagcttc ttcttctaag
     5281 ttaaaactat gtcttaacat catgcttaca gatagaatag tcgcaattgg atttacaata
     5341 tcttttccag ctatatcagg tgcacttcca tgtattggtt catacatacc aaaactatct
     5401 tctctaagtg atgctgatgg taacatacct atagaacccg taatcataga tgcttcatca
     5461 gatattatat ctccaaatat attattagtc actattacat caaattggct agggtctttt
     5521 acaacttgca tagcagcatt atcaacatac atatgtgaca gttctacatc ttcaaactct
     5581 ttagctaagt cttccattat acttctccaa agtcttgaac tctcaagcac atttgcctta
     5641 tctacactta taagtttttt atttctattt ctggctattt caaaagctct cttaccaatt
     5701 cttcttattt cattttcgtt atatatttct acatcataag cattttcgat accatcaatg
     5761 atttttcttc cacgttctcc aaaataaatg ccccctgtta attcccttac aacaacaaaa
     5821 tcaatgcctt tctcaactat atccgttcta agtggtgaag cttcttttat actttcatac
     5881 attgtagctg gtcttaaatt tgcaaataaa cccaatgact ctcttaattt taaaagacca
     5941 gactctggtc ttttatctcc cttacattta tcccactttg gtcctccaac agcaccaagc
     6001 aatacagctt cgttttttcg acaagtttca acagttttat taggtagagg ttctccagtt
     6061 tcatcaatcg cgcatcctcc agctaataca tattcacaat cgaatctatg attaaattta
     6121 caacaaattt tatttagaac ctttatacct tcatctataa tttccggtcc aacaccatca
     6181 ccttttatga ctgctatatt acaattcata cttcactacc ccctatttgc ttttattgag
     6241 ttaattaagc cttcatttaa tataatattt tgcataaact caggaaatgg ttctgcttta
     6301 tattcttttc cttttgttat attttttatc acacctgtag aaaaatctac ttctatatta
     6361 tccccttcat ctatgttatt ggcagcttct tcacattcaa gtataggaag ccctatattt
     6421 atagagtttc taaaaaatat tctagcaaaa gtagaagcaa tcacacagga tactccactt
     6481 tcttttatta caattggtgc atgttctctt gatgaaccac aaccaaagtt tttagttgca
     6541 actataatat ctcctttttt taccttagaa ataaatttat catcaatatc ctccatacaa
     6601 tgcgttgcta gttctttata atcagctata tttaaatatc tagctggtat tattacatct
     6661 gtatctatat tgtcaccgaa tttaaaaaca cttccattgg caatcataaa ttttacctcc
     6721 cttaaattat ttctgatggt tttgatattt ttccagttat agctgatgct gctgctacag
     6781 ctgggcttgc aagatacacc tctgaatcaa catgccccat tcttcctacg aaattcctat
     6841 ttgttgttga aatggatttt tctcctgctg caagtatacc catatgtccc cctagacatg
     6901 gaccacaagt tggtgtagaa actgctgctc ctgcctctat aaaagtggta atataccctt
     6961 tttctatagc ttcaagatat actttttgag ttccaggaaa tatcataact ctaactccat
     7021 ctgctacttt tttgcctttc attatctcag ctactacttc aaggtctgat attcttccat
     7081 ttgtacaaga gcctattaca acttggtcta tctttatatc cttatctatc tcatctactg
     7141 ttctagtatt ttcaggaaga tgaggaaagg ctactgtttc ttttagttca gctaaatcta
     7201 tttcgtatac ttcatcatag actgcatctt catctgcttc atatatattt acatcttttg
     7261 tcatagttga acacttatgt gaatttatat attccatagt cttatcgtct actggaaaaa
     7321 ttccattttt tgctcctgct tctattgcca tattacaaat agtaaatcta tcatccatag
     7381 ataagtattc tactccatct ccacaaaatt ccattgactt gtaaagtgct ccatcaacac
     7441 ctatctcccc tattatatga agtattatat ctttaccact tatccattta tttggtttat
     7501 tcttcaaaac aaattttatt gctccaggaa ccttaaacca acattttcct gtagccatac
     7561 ctgctcccat atcagttgaa cctacaccag tagaaaatgc tcctaatgct ccatatgtgc
     7621 aagtatgaga gtcagcacct ataattacat ctccagcagt tactatacct ttttcaggaa
     7681 gaagaacatg ctctattccc atatctccta catcaaaaaa atttacaact ccttttgatt
     7741 ttgcgaaact tcttgtaaat ttacattgtt ctgcactctt tatatcttta ttaggtgtaa
     7801 agtggtctaa aaccatcact actttagtct tatcataaac atcttcaatt cctaattttt
     7861 caaattctct aatggctact ggtgatgtta tatcattacc taaaactaca tctaaatctg
     7921 cttcgattaa ttgccctttc ttaacttcat ttaatttagc atgtttggct aatattttct
     7981 gcgtcatagt catccccatt ttttatttcc tcctcaatat aacttttatt tattgcattc
     8041 ataagtgcat taatagctgc tgtatttatg ttttccgata tacttacacc aaaatagact
     8101 tcattattgt gctctattcc tacataagct acccctcttg aatgtgaccc ttcctctaat
     8161 gcatgttccc agtaatatct aaatttataa tctttcatgc cacattgctt tagtgcatta
     8221 ttaaatgaat ctacaggtcc atttcctata ccaacaatcc tttgttcatt tcccatataa
     8281 cttattcttg cagtcatctc aatcatgtta gtatcattag aatcatcatt ttcatagttt
     8341 aattcatcca ttgattttat tttatacttc ttaactttat aagggctctc aacttcaata
     8401 tattctttct taaacaaatt aaatattgct tcactactaa gttcttcccc aattctatca
     8461 gattccattt tgactacatt tccaaaatca gaatgcatat tcttaggtat cataaatcca
     8521 taatctgttt ccattatata aacagctcct ccttttccag attggctatt tatccttata
     8581 atttctttgt attctcttcc aatatcatga gggtctattg gtaaatatgg aacttcccag
     8641 taatcattat ctctgttttt cattgctatc atgccttttc taatagcatc ttgatgtgaa
     8701 cctgaaaaag cacaatgtac aagattacct gcatatggat gtctatcatg taccataagt
     8761 tttgtacaat cttcatatat acctattatc ttatctatat tgctaaaatc tagctctggg
     8821 tctattcctt gtgtataaag attaagtccc acatttacta tatccatatt cccagttctt
     8881 tcaccatttc caaatagtgt tccttcaagt ctatcggcac cagccaataa tccaagttca
     8941 cttgcagccg tacaagttcc tctatcattg tgagtatgca aacttagtat aatagaatcc
     9001 ctacaactta tatttttaca aaaccattct atttggtctg catatacgtt tggtgttgcc
     9061 atttcaactg ttgatggaag atttattatt actttttttt gagtagttgg tttccaaaca
     9121 tctatcactg cctcacatat ttcaagtgca taatccatct ctgttcctgt aaaactctct
     9181 ggtgaatatt caaaagtaaa ttctgtttct ggatattttt ttacttcttc attaaataat
     9241 tttgctcctt caactgctat atctattatt tcttgtttac tcatattaaa aaccacatct
     9301 ctttgaagaa cagatgtaga gttatataaa tgtataattg cttttttaca tccccttatt
     9361 gattcaaatg ttttttctat aagatgagcc ctagattgtg taagtacttg tatagttaca
     9421 tcatctggaa tcatattctc atctattaat tttcttaaaa atgtatactc tgtatttgat
     9481 gctgatggaa atcctacctc tatttcttta aatcctgtat caataagcat tttgaacatt
     9541 ctgacttttt catttacact cataggtgtt ggcagtgact gatttccgtc tcttaaatcg
     9601 acactacacc aaattggagc cttaaatatt tcattatctg gccatttacg atttactatt
     9661 tgcattttat catatttttt atactttcca cacttcatgt tatttccccc tttttctgtt
     9721 ttttgtattc agtatacctc atatcgacgc aatcctgcac aattcataag gataaatgtc
     9781 aattttatca ttagaataaa aaaaacctca tctctaataa aattagagac gaggttttgc
     9841 tcgcggtacc actctattta gctttctaaa aagctccctt agtaatagcc aatacaataa
     9901 ttataatgac tatatttcct ataacgtgga attactccga ttattctaag atttaaatct
     9961 tagaaaacag ctcctagaca agttcagaac tatcaactac taaatcacac catcctttag
    10021 ctctctgaaa gtttcattgt tcttactact tctattcatc acttttttaa tcatttaaat
    10081 tttctaaata ttttgattta atagttatta tatacacatt aaaatgtatt gtcaacacaa
    10141 aaaataatta tctcaatttt ttatttttga taagcaattc tataaaacat gctaactatt
    10201 gaaaacagta tgatattatg ccttaaactt tttcatcttt tcatctatga tattcaataa
    10261 ttttttttca tcttccaaac tcaatgccct taatctaatt ttataattag attcgtcagt
    10321 gtatatattt aatatatttt ttactacttc tattttttct attatcttat aattaaactt
    10381 ttgactttgt ttagatttta tactgaatat acattgatta tttattaata ttgaatgatt
    10441 atcgagagta atatatactt ttccctcata tttatttgaa aaatcacgac cttgtttaaa
    10501 tagaaaaact gctgttatta tacataaaat atataatatt gtcgttgaat atataaaaat
    10561 tgtagctaaa attataagtg ctaaaaccat atatatagtc attccttttt taaaataacc
    10621 attgtcataa taattcatat agataaactt atttttactc aaattttttc acttccaaga
    10681 ttctaaaaat ttatatcaaa aaattctata atttcatctt catgataatt tatgactaat
    10741 tcatctggaa aatctactgc ttctaaaact ttttctgcat tttcaaattc tcctatagaa
    10801 taacatatat gagagtcagt accaagaatt actcttgcac catatgtttt acacaatgtc
    10861 aacatatgac ttacattttc ccaagtaccc gttctatgag agtttgaact taatgatgaa
    10921 ttatttattt ctaaaaggat atttttatct ttagcttttt ttactatagg ttcataatct
    10981 aaaggatacc tactgtcatc tggatgtcca attattttta ccttaggttt atccataact
    11041 tttaaaatag catttgtatt ttcttctttt gtaccagaag ctatacaagg tctatgcaaa
    11101 cttgcgatta tataatccaa atgttgcaac atatcttctt gaacatctaa attaccatta
    11161 taatccatta tatttccttc tattcctcta agaatcttta cgccttgcac ttcacgtgga
    11221 agcaaatgca aattataaaa atagaatgga tgtggtcctc ctggcatatt aggtccatgg
    11281 tctgatattc ctaagtattt tatattcttc ttactcgcct cttcgacatt ttccttgaaa
    11341 gtactatgtg catgtccact tattagagtg tgacaatgta catccataat agactttttc
    11401 attaaatttc ctcctaatat tttacataaa ttcgtgaaaa tagctaaagg gctatttcaa
    11461 aactaaagct gtcataatag acagtgtaac gttttaaaac agccccattt tcaattattt
    11521 tacacttcta aatctttaaa tttatcttta aataattctc caatacttcc taaagattcc
    11581 tctacttcta ggtattctgt tatatcctct tctggctctc tagttgcttc ttttattgaa
    11641 accaacattc tcttagtctt tggtttaaaa tcaagaatct taactttaac ctcatctcca
    11701 atattaacaa cattagtaac tttagccact ctagtatcac tcaattcaga tattggtaaa
    11761 tacgcatcta caccttcttt aatttgaaca aaagcccctt tttctattat tctaagaact
    11821 ttaacattga caacatcatc aatttgtact tcttttgcta ttaaatccca agggtcatta
    11881 tttatgtctt ttaatgccaa ggcaattttt ttagtctctt tatttacatc caacacataa
    11941 acttctactt cttgtccctc tgaaacaaca tcttcaacac tttcaactct tgtccaagct
    12001 aaattattta tatgtgctaa tccttcaatc ccaccaatat ccacaaatgc accataaggc
    12061 attatctttg tcaccttacc atctcttttt tgtccaactt caagagaatt aaataagtct
    12121 tctttagctg ctttgatacg agctttttct tcttctcttt cttgcttaat tcttgctctt
    12181 tcagcttctt tttctgcctt aatttgagct cttctttctt ctctctcttt atttatacgc
    12241 tcttgtaata catctctatg agatgctaca agtctatttt ttctaacatc caattctttt
    12301 atataaactt ctaagttaag accaacaaat ttagatgtat ctgtaataaa ttttgtatca
    12361 agttgagaaa ttggtataaa aaatgtatat gtattgtaat tagcaaatac acctttatct
    12421 atattctttt caacatttac agttattatt ctatgttcat cataagcctt ttgtaactcg
    12481 gcaaaatcat ttcttttgtc aagttgtaat ttagataaag ttattgtacc atctttttga
    12541 gatacttttg ttattacagc agttatttca tcacctatgt ggtatatatc ttctatatat
    12601 tgattttttt ctatgttcaa ttcacttata ggtattattc catcaaaccc atagtctaat
    12661 cctaattgta cttcatcatt tctcactgca gttatttttc cagttgtcaa ttctccaact
    12721 ttcactttgc tgaatacttg ttcttgctgg tcaagtaatt cttgcattgt taaatcattt
    12781 tccattataa acatcctttc ctcttattga ttatcatatc tgtaaatatt ataccattaa
    12841 tattgcaaat acttacattt ttcttactat aatttttaaa ataaattcaa cttcatatat
    12901 ttatacatta tagaaaatat cttattaaat taaaatacaa attctaattt ttatctaata
    12961 aaatattttc ataaaaaatt tatattgtat atcttattct tttattgctt taggttttga
    13021 tagaaaacat ttataaaata taaagaaaga aattgctgtt atagtagcgg ctgttatatc
    13081 tgcaactggc tctgctaaaa ttactgcaaa aagtttttca ttaaagaatg ttggaagtat
    13141 aaatattaaa ggtattaaaa gtacaatctt tctaagtaga gctaaaacca aagatattgt
    13201 tgcttttcca agtgctaaaa atgtctgttg acaagctatt tgaatcccaa acataaaagc
    13261 accagcaaaa tatattttta tactccaact agtcatacta agtaactgcg gattcttatt
    13321 aaatatacta acaaaaaatg caggaaatat cattaataaa agccataaaa tagtagaata
    13381 tgtaaaacat gacaataaac acaacttaaa tgtctttttt actctatcaa gttgacctga
    13441 accataatta taacttatta ttggttgtgc tccttgtgta acccccatat taggaagcat
    13501 aagaatttgc attatactgc tcattatggc cattgaacct actgctaaat ctcctccata
    13561 catttgtaat ttactattta aggctataag tactaaactt tctgtagatt gcattataaa
    13621 tggagagatt cccaagccaa gcactggtaa gatgatactt gcttttggta tcatatactt
    13681 cttttttatt ttaagtatac ttttctttcc aaagagaaac ataagtaccc aaatagcaga
    13741 aacgccttgt gcaattatag tagctaatgc agctcctttt acacccatat taaagccaaa
    13801 tataaaaatc gggtcaagta ttatgtttat taaagcacct atagcaactg tcatcattcc
    13861 agttgtagca aacccttggg tatttataaa agtattcata cccattgaaa tctgaacaaa
    13921 cactgtacct attaaataaa tatttaaata gtccattgaa tatccaattg tagattcact
    13981 tgctccaaac atccaaagta aaggctcttt tccaatctta aaacctatag tcaaaagtat
    14041 tgctaaaatc accaatacag aaaaactgtt actcattatt ttctctgcct catcattgtt
    14101 ttgctcaccc atttttatag cagctaatgg acctcctccc ataccgatta aataactaca
    14161 tgcagttatt ataattatta ttggaaatgc cactccaaca ccagccatag caacttctcc
    14221 attttccatt ctaccaataa aaatcctatc gactatgttg tataaaacat ttacaatttg
    14281 tgcgataata gcaggtgtag caagtttaaa taataattta cccacacttt cactacctaa
    14341 gttagtgttt gaattttcca ttttaaaacc tcctcaatcc aataattaaa atgtatcact
    14401 aaaattttat tctatataaa aatttcattt tattagcaga caaatttaga gtactttccc
    14461 tacaagtaat actatactat atttcttttg ccatagtaaa cattttagaa ctaactgaag
    14521 ttaaatactt tatctcattt tttatttatt attaaaaatt tttcacatat actcataaaa
    14581 aaactatcca agtatattac ttagatagtt ttatattata tgtaaagaaa gagctgaaac
    14641 tataacccct gataacaaat tgccaagaaa tactcccata aaagcatctt ttattctcat
    14701 ttttaatatg gaagctatag cagatgcagt ccatgttcct gtagttggca gtggtatacc
    14761 tacaaatagt attataccaa ttatcgaaac actttttaat ttcttcattc ttgagtttat
    14821 ttttctatca attacatcag caatcccttt aaatagtttc ttccctctta aaaattgtaa
    14881 tacatgtctg aatgtcaata tcaagggaat cgataccaat gtagaaccta ttacactaaa
    14941 tacataaacc catataggat ttaagcccat tgcaatacct ataggaatgg ctcccctcaa
    15001 ttctgttaca ggaaccatag ataaaattaa tatgtgtaaa tatcccaaac tattccctcc
    15061 tattctaagt gacattaagt tatgaattta tttgctctat aacctcacca tcctttttac
    15121 taactaaaaa tgcattttta gtcaatgaaa gcattggtaa atcactctta gagtctgaat
    15181 atgcatatga attttcataa tcaatctcta tcccaagttt acctaataca gcctttattc
    15241 tttttacttt ttcaattccc ttacagtttt ctccaatgaa ttgattttta tatcttgaat
    15301 tttcataaaa aaactccgtg cctataacct cgtctgcaag accataatac ttaaaatatt
    15361 tcatatatgc atatggagat gcagttatca taattataac attatcttct tctttatttt
    15421 tgtaaataac ctttttcata gattcgttaa tttttttcat tatgcaatca tcaaaaaact
    15481 tttttaaatc ttcttcacta aaatctacta ttggataaaa gattgcctct tttgccttct
    15541 tcaagggtat cattctaatt aaatacataa atagaataaa aataatgtat ggcaacttta
    15601 taatagtttt aaaggtttta ttttttaaag agtaaataaa aaaatctatc atgctatcag
    15661 tatttataac agtataatct acatcaagta ataatacact ttttttcata agtatttccc
    15721 catcctatca ctaaaaatat cactaaaaaa tcaatattat taaacatgta attatccata
    15781 agattacgtt tatttttaaa gcattatctt ttaggacaat atcagtagga ttattttcat
    15841 ttgtagtgtt aattatataa ttatatctaa gtacaccata aactacaaat attgttgtaa
    15901 atatcatatg tggcttttct ggattacttg tagaatacaa tgcataacat acaattgtag
    15961 aacttaatat aactatcatc atttgattta gattttctat gtcataatct tccaatattt
    16021 ttctatgttc aactgcatca tctcttagag tttctatttc ttttttacgt tttccaagag
    16081 ctaagtacag agataagaaa aatgtacata aaattatcca atttgataaa ctaacactta
    16141 tggctacaca tcctgcataa accctaagta taaaaccaag agatattgaa aacacatcta
    16201 atagtattat attctttagt ttaagagaat ataatatatt gtttatcaaa tagagactga
    16261 gaattataga aagattaaaa ctaacaactg ttgataaaca gactgaaatt aacgctaata
    16321 ctacaaatag tatattagca ctttttttgc ttattcttcc acttgccact ggtctattac
    16381 attttttagg atgtttttta tctttatcca tgtctatagc atcattcagt gcatatactg
    16441 atgatgacat taggcaaaaa cacacaaacg caataaacgt atttaaaaat aaatttatat
    16501 ttagtatgtt gtttgaaaaa attatagctg aaaatacaaa gaaattcttt atatattgct
    16561 ttattctcat taactttata taatctgaaa ttttagtaag aagccttata cttgtattgg
    16621 tctgtttcat aaacttacta ccctttcaat attatttttt aaacaggata tgattttatc
    16681 cctatgttta aaatttctat aaatccattg acattactga ttactatagt aaccaataac
    16741 agcgatagaa atatattata catattcttt tcattttttc taagggcttt cgtaaagtaa
    16801 gccaataaaa ttataactgc aaaactaaaa tttatagcca taatacttga attgactaga
    16861 ttatatccta ctataccatg taaagttaga ttgaatataa ttattcccat acatagctta
    16921 ggaactcttt tttctgaatt ttcaataact gataaaacac aaaaacataa agctaataat
    16981 gtaagaatta ctggtattgt agcaacttct ccattttgca aaaataaccc ttttttaata
    17041 cttgctccag gaaaataaaa aaccgaatac aataatctct ctataatcat tgcaatcttt
    17101 tccaaaattg gttctccact tataaatttc gtatcatact gtattacatt tccaaggttc
    17161 ataaaactct cataagactt cgttgaaact acacaaatat atgccattag gataaatgta
    17221 attaaatgtt ttaacatttt tcctattttt aatttaaact caaatatcat att
//
LOCUS       DAYAOX010000076        17176 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.076, whole genome shotgun sequence.
ACCESSION   DAYAOX010000076 DAYAOX010000000
VERSION     DAYAOX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.076"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            162..551
                     /locus_tag="ACGI57_001904"
     CDS             162..551
                     /locus_tag="ACGI57_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9716983.1"
                     /translation="MKQQEVVNKLRRGELMLMEYLWRKNSILSKKEIIEAMKEKYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNAIKEDRKVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIENMEKIGKE"
     gene            645..1028
                     /locus_tag="ACGI57_001905"
     CDS             645..1028
                     /locus_tag="ACGI57_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9716984.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDGNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEENLSDDKLDMLEEWVKNWEEDE"
     gene            1746..3137
                     /locus_tag="ACGI57_001906"
     CDS             1746..3137
                     /locus_tag="ACGI57_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9716985.1"
                     /translation="MRNKIIIGITFLLVLLMSGCASKQTAEINVLATTDLHGVIPYEL
                     TSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGDNNTESYIETPISK
                     DMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTYKKNGDSYTKPYII
                     KNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVE
                     DAKKWVSIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVDGIDAIVAGHNHVQ
                     IEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDKSSKLIKFEKNQES
                     DKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDIYKTIGYKWRNISS
                     PKSDGMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKDNVVTIYPNKNDKFKIEK
                     GKQEYSINLKHIQ"
     gene            complement(3285..3593)
                     /locus_tag="ACGI57_001907"
     CDS             complement(3285..3593)
                     /locus_tag="ACGI57_001907"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9716986.1"
                     /translation="MLREQGSAIRDTLDSVLSLSNQKAYPVWESVNSFALIKAAQAGL
                     GITILPENLLLDSLLHKKLRLIELDGIDMENKMLAILHKDKNITQPLKIILDNISNVL
                     "
     gene            3630..4151
                     /locus_tag="ACGI57_001908"
     CDS             3630..4151
                     /locus_tag="ACGI57_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFG9716987.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKIFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIMSGILGIFFYL"
     gene            complement(4280..4516)
                     /locus_tag="ACGI57_001909"
     CDS             complement(4280..4516)
                     /locus_tag="ACGI57_001909"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9716988.1"
                     /translation="MLECFIAKDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSKKFCQEAYDWFVENQ"
     gene            complement(4826..8254)
                     /gene="uvrA"
                     /locus_tag="ACGI57_001910"
     CDS             complement(4826..8254)
                     /gene="uvrA"
                     /locus_tag="ACGI57_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFG9716989.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEALNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNHLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQAPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            8573..10807
                     /locus_tag="ACGI57_001911"
     CDS             8573..10807
                     /locus_tag="ACGI57_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9716990.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            11063..13093
                     /locus_tag="ACGI57_001912"
     CDS             11063..13093
                     /locus_tag="ACGI57_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9716991.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            13090..13542
                     /locus_tag="ACGI57_001913"
     CDS             13090..13542
                     /locus_tag="ACGI57_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9716992.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            13544..14599
                     /locus_tag="ACGI57_001914"
     CDS             13544..14599
                     /locus_tag="ACGI57_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFG9716993.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            14666..14974
                     /locus_tag="ACGI57_001915"
     CDS             14666..14974
                     /locus_tag="ACGI57_001915"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFG9716994.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            15078..16343
                     /locus_tag="ACGI57_001916"
     CDS             15078..16343
                     /locus_tag="ACGI57_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HFG9716995.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            16431..17147
                     /locus_tag="ACGI57_001917"
     CDS             16431..17147
                     /locus_tag="ACGI57_001917"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9716996.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHELEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
BASE COUNT         6448 a         1953 c         2838 g         5937 t
ORIGIN      
        1 aattatttga aaatttaata attttataga tatgaaagtt attttcttct aagaaaacag
       61 actaaatgcg tagaatattt ggtaaaactt acctgtataa attgaaatca gaaggtttta
      121 ttattgattt agattagaac aatttgaggg agtgacataa aatgaaacaa caagaagtag
      181 ttaataaact acgcagggga gaattgatgt tgatggaata cttgtggagg aaaaattcta
      241 ttttatctaa aaaagaaata atagaagcaa tgaaagaaaa atataagtgg aggaaaagta
      301 ctacagaaat tctattaaaa agattagtaa aaatgaaaac attaaaaaag aagaggatag
      361 gttttcagtt tagttatgaa gtgctagtaa ctaaaaaaga atatttaaat gcaataaaag
      421 aagatagaaa ggtaactaaa tatgatagtt tttttatacg aatgtttacc actatgcata
      481 aaaaagaaaa gatgacagaa gaacaaataa aaaaatatat agaaaatatg gaaaaaattg
      541 gaaaagaata atttatatat atattaatta tttttaaaat aaatttgtta taatatttaa
      601 atatacataa acatacataa atttacaaaa agggggtgta gcttatggga ttcaagaaat
      661 tgccagattc tgagttaaaa gtaatgaagt ttatttgggg attagatgga aataaggtag
      721 catcaggaga agttgtggaa gcaatgaaac aaatttatga ctggaataaa aatacgacat
      781 taacaatatt gtctaagctt gcaaagaaaa actatttgta ttctcaaaaa acaagcaagt
      841 gtacatatta tactatcgct gtaagagaaa aagattattt aaaagttgaa acaaaaaaat
      901 tctttagttt ttttcataat aattctttaa aaagtttttt cactgcttta aatgatgaag
      961 aaaatttaag tgatgataaa ttagatatgt tagaggaatg ggtgaaaaat tgggaagaag
     1021 atgaataaaa tgaatgtgat actaaaatga tactaatatg ttttatagcg tgttaaaaaa
     1081 tatagaaaat atagaaataa tagaagaaaa ttaatgtaat agataaggtt ttatcagatt
     1141 aaaaagaaaa tcaatagaat gggaataaaa tatagaatgc caaaaatcca cataaataca
     1201 ggatttaagg tctctcttac ttctatgtta tactggtttg atactatcaa tatttgccag
     1261 tatcttatgg attaataagt aaaaaacatc ctgctgaatg ataaattcag cggggtgttt
     1321 ttattttgtt ttagaactcc tttgctctga aactcgaagt atttaacact gatgattttg
     1381 ttatctttta gttccttttg tgtcatgctc tgaaagacaa attgatacaa taaaaaagta
     1441 atttcttaat cattgatgga gatatgtatt tatgttactc ttgatggtaa tttgagtaat
     1501 attgttcata gctttttata ttcaattaat tagaaaacca tttggtataa tcataaataa
     1561 acttgatgat ataatagaaa agatttataa ttattagaag tatataaaag tattttatgt
     1621 ctatgaaatg tatgtctata aaacatatag atgacttttg tatattaatg tatgaatatt
     1681 gatattatag aagaattgag ttgtcaatga tactaaatca aatatttata gaagctggag
     1741 ggattatgag aaataaaata attataggaa ttactttttt acttgtatta ctaatgtctg
     1801 gatgtgcttc aaaacaaacc gcagaaataa acgtattagc aactactgat ttacatggag
     1861 ttatacctta tgagttaact tcttatgtaa aagaagaaag aaaaaaagat aaaaatctaa
     1921 ctttagtaga tgcaggagat ttttttgata gtggagaagt gtttggttct agcatggata
     1981 gttattttag tgctagatat aaagatggtg ataataacac tgaaagttat attgagacgc
     2041 ctatttcaaa agatatgaaa gaggtaggtt atgatgttgc agttcttggg aatcatgaat
     2101 ttatatccaa caataaattt tatctagata atatgatatc tgattttgag aagcaaaaaa
     2161 taaatatttt atcggcaaat acgtataaaa aaaatggaga cagttataca aaaccataca
     2221 taataaaaaa tattaataca cctgaaggaa acgtaaaact aggaatttta gggcttacta
     2281 taaaagaagt tggtgagagg aaacagtgga aagatggaaa acttgttgac tctaagtctt
     2341 tagaactaaa agaccaagag ggctacaatg gtgaattgta tatgaatgat ttagtagaag
     2401 atgctaaaaa gtgggttagt ataatggaaa aagagaagcc tgacattata gttgctgtgg
     2461 cacatacagg tgagaaacct aagaaaccta aaaacccagg aaatagaata caagacttag
     2521 ctcaacaagt tgatggaatt gatgctatag ttgcaggtca taatcatgtt caaattgagc
     2581 aacatgatta taaaaataaa tcaggagaaa atgttatagt tactgagcca ggaaagcatg
     2641 gggaatgtat ttctaagatt aattttaaat tagaaaaaaa caatgataaa tggaatatag
     2701 ttgacaaatc tagtaaatta attaaatttg aaaaaaatca agaaagtgat aaatttggag
     2761 aatttatttc ttcaattaat aaagtaagta aagataagaa agaaataaga ttatcagaag
     2821 taaagactcc tgaatgggat aaaatatatg tttttaaatc tggaacatct gcagatgata
     2881 tatataaaac aataggatat aaatggagaa atatatcatc tccaaagagt gatggcatgg
     2941 tgcagattgt tgtaatgaat aataataagc ctgttagcta tatatatggt gaagctgaag
     3001 ttatgccaat taatataaaa tgtgatgaat cagagtataa agataatgtt gtaactattt
     3061 atcctaataa aaatgataaa tttaaaattg aaaaaggtaa acaagaatat agtattaatt
     3121 taaaacatat acaatagaaa cttttatctt atttaagttt agattgtact taaaataaag
     3181 tgtgtattat tttaaaaatt gatgataatt aaaatcacaa cattaaggaa tagtacacac
     3241 tttttatgct aaaaaataga atgttaaaaa attaaacctt taaattaaag tacattgctt
     3301 atattatcca atattatctt caaaggttgt gtaatatttt tatccttatg taatattgct
     3361 aacattttat tttccatatc tatcccatct aattcaatca atcgcaattt tttatgtaag
     3421 agtgaatcta ataataaatt ttctggcaaa atggttatac ctaaccctgc ttgtgcagct
     3481 ttaataagtg caaaagaatt aacactttcc caaacaggat aagctttttg gtttgaaaga
     3541 gataatacac tgtccaaagt atcgcgaatg gcacttcctt gctcacgtaa caataacgga
     3601 aacgtgcata actgtcgtag agataaggct tgtttagtat tggtggtggt tatgcaatca
     3661 tacctttaat ccaagagaaa gtagtaaatc aaactggatg gataagtgag aaaatattta
     3721 cagatattat aactatttca caaatgacac cagggccact ggctgttaat acttctactt
     3781 ttgttggact tcaaattgga gggttttggg gagcagttgc agccacttta ggttgtgtat
     3841 tatgtggtgt agtaatatct ctatttttat ataatttttt tcaaaagcat cagaactcta
     3901 cttatatatt tgaaatatta aatggtctaa aatcagcttc attaggttta attatctcag
     3961 ctgcggctac aattataatt cttgcattat ttgggacgaa taaagtgtac ttcaattcta
     4021 tatttgagat gctaaactgg actgcactaa taatcttttt tgttacacta ctgatagtac
     4081 gtaagtggaa aattaatcct attgtgatta taataatgag tggtatactg ggaatatttt
     4141 tttatttata agtttttgct aagtaaagga tatctatttt tttatatctt aaatccaaat
     4201 agccaataaa gtaaggctat ttggattttt aataaattct gaaaagataa tcagaaacct
     4261 atataacatt taaattatat cactggtttt ctacaaacca atcataagct tcttggcaaa
     4321 atttcttact atctatatag taactattat gaacattatt aatttcaaca agtttctttt
     4381 tacattttag tgaattataa tagctatcaa aatattgggt tttaaaatat ctatcatgaa
     4441 gtgttttaaa gacaagagta ggtatgttga ctccatcttt tatcttacta tcttttgcaa
     4501 taaagcattc taacattgta ttaattatct ttgtactatt tttccaaata tgaggatttt
     4561 tcttttcaaa tattttataa aattcattat catagacttc attataacct ttatatttag
     4621 gtggttttac tgttatgtga aaattaggaa gaaacttaaa gaaaaaatat aataattttt
     4681 atgttctgtt ttatatatta ttcatatttt atcacagata atatactaaa gttaattttt
     4741 ttattgtaca actattataa taattcttct ataatataag acattctata attttatatt
     4801 taagttcatg tgtatttaat tgtttttata taaaatgtcc agtaatacta tttggattta
     4861 tttttatttc atcaggagtt ccagaagcaa caatttcccc tccatttatt ccactgtctg
     4921 gacctatatc gataatccaa tcagaagcac gtataacctc tgtattgtgt tcaataatta
     4981 aaagtgaatg ctgttcattt acaagttttc taaaagtaat taacaaattt tctatatctt
     5041 taaaatgaag tccagtagta ggctcatcca atatgtacag cattcgtttt ttagtttttg
     5101 tccctaattc ttttgctaat tttatccttt gagattctcc tccacttaat cctaatgtat
     5161 tttgtccaag ctttagatat ccaagtccca cttcatctaa taaggagagt tttctatata
     5221 tatcagagca atcgctaaaa aatgtttttg cttctgctac ttccatgtca agtatatctg
     5281 caattgtctt tcccttgtat tgtatagaca gtatttcttt ttgatatctc ttaccttgac
     5341 attcactaca agttacccat tggtctgcca tatattgcag aataattttt gttttaccta
     5401 atccttgaca gtttgggcat tgcccctttt tgctattaaa gctaaaatag ctttcatcca
     5461 ataatttttg ttttgcttgt ggagtttcag caaatagaga acgaatttca tcaaaaatat
     5521 tgatatatgt agcaggtgta gaacggctag attttccaat aggtgcttgg tccattaaga
     5581 taaaatcatc aatagcatca aatccagtaa attcagtata atttttatct gggatagatt
     5641 tttgtttcaa tttttcttct agagcaggaa gtaaactgtg aaatacaaga gagctcttac
     5701 cagaaccact aacacctgta atagagcaca tacagtttaa gggaatttct acatcaatat
     5761 tttttaagtt atttgcatgg catcctttta agcttaacca tttacttggt ataattttat
     5821 tttgtgaact tattttattg tatgtactta aatatttacc tgttatagag tttggatttt
     5881 ttataatttc ttgggttgta ccttctgcga taatgaaccc cccttttgta ccagcacttg
     5941 ggcctacatc aataatataa tcagcagaca gtattgtatc tttatcgtgt tccacaacta
     6001 taacagtatt acccttatct cttagctctt ctatcatttt gatgattaaa ttatggtctc
     6061 ttgggtgtaa accaatagat ggttcatcta aaatgtaggt taagcctact aattcacttc
     6121 ctagctgact tgataatcgt actctctgca gttctcctcc agataaagtt ggagctgttc
     6181 tatctgttga tatatagcta agacctacct ttaatagata actaactcga atttttaatt
     6241 ctgttaagat atcatttacc aaagagagct catttttttg aaggtgattt ggtaattcac
     6301 acaaccaatt ccataactgt tctatcgtca tttttgttag ttcagggaag cgataacctt
     6361 tgattgtagt gaatctagcc tcaatgcaca gcaattcgcc accacatgta ttgcaaggaa
     6421 ttttgttcat atattgatgc aaggtattat tttcagaact agattctcta aaaagtcttt
     6481 gaatgtggtt gattgctcct gaagcaggtc ttctaatctt tgattctcgt ccctttgatt
     6541 caaagctaaa ttcatatatc ttatcgtctg taccatataa gattgcatct ataaaatctc
     6601 ttggtaattc attccaaggt atatctaggt ctatattttt atcttttgct attgcatata
     6661 attcacctac catccaatta ccatttggct ttttccccct caattttcca aaatagtaag
     6721 tagctccatc taatattgaa atagttgggt cttttacaat taattgagga ttaacttgat
     6781 aggtaaaacc taatccatta caatcgtgac aagctccagt atgggagtta gcattgaagg
     6841 ttgaagtatt tatcttagaa aaaatagttt gacaatgtgg acaataattt tttgtattat
     6901 tctttttttc taatggaatt ttgcaatatg gacagtaact ttttccgatt gatacaaata
     6961 acattcttaa ataatcccaa atgcttgtaa gagtaccaac agtagagcga ggattttggc
     7021 ttcctttttt ctgttcgatg gctatagtag gggttaatcc ctgaagtaaa tcaaattccg
     7081 gtttttgaag ctgttctgaa agttttgctc cgttacttag attttcaaaa taacgtcttt
     7141 ttccttcttc ataaatcgta tcgaaagcta aagaagattt tccactacca cttacacctg
     7201 taataacagt tatttgattt tttggaattg taacttcaac atcctttaaa ttgttttgcc
     7261 ttcctccttt taaatagata gagcttttat tatgacaaag tgaattttta attgatttat
     7321 ttattgcttt tgtaaatttg aatttctttt ctagttcaag aggattgcct aattctttta
     7381 tttccgaaat acctaatatt tttaactgag aaaagattga tttcaaataa tcttcaggca
     7441 atttttctat taacaaatta agggcttcta tttttatatt tccgcttgat aatatctctg
     7501 ccatttcatc taataaattt ttttctgctc ttgttaattg attgtatttt ttggaactta
     7561 attctgtcca taaatcccat ggaagcaatt ctaaaccaat catattatta aagtcatgta
     7621 gaagattacc ctttacagaa tgccaacctt tccaattttt attatatcca aagtcatctg
     7681 gatgagcatg tccttgccga catcttatcc aggcttctga accagttaaa aaaccatctg
     7741 tttttccgac atcatgagtg tcaaaattaa tttgaaaata atttttttgt atgtcatcta
     7801 tttgtgcatc tattcttatc catctctttg ttagagtatc atagtattct acaagccagt
     7861 ggtcttcata agtaagctca ctttcaaaat aattagcaaa acctgctctc attctagcct
     7921 caaaacctcg atatcttagg agcgatacaa gaagtaaaga ataatctctg cacattccaa
     7981 caagcctttg ttttgggttt cttggaatag ttagtgggtt agaatttatt tcaaatattc
     8041 tttttaacat ttgaggaatt gtacgaagca attcctcatc agattgttgc ttattaaagg
     8101 atataccata taattttcct tggtctccat gaattactag gctttgaacc acttttaata
     8161 actcaattgg agtctttggt aaatcttgat atttattttc aaatttttta ggatttgtga
     8221 tgatactttg atttttatag ttatttattt tcatttatat ttcacctctc taaatggatg
     8281 atataccctg acatggtgtc atatgcaagg acaatttaga tttttggcca aaatttaaaa
     8341 aataaataaa aaatgataca gtgataaatt gtaatacaaa tatagtttca aattgcatat
     8401 aatataatta tttaataaaa ccattatcta ttgaatgtta atgaaatgtt aagatggcat
     8461 tcttgaatat taagaaatag aatactataa tatagtgtga aggattttta gtaaaaaaat
     8521 ttgtaagtgt taatatacat taaaaaatag gtttataatt tggaggaaaa aaatgaaatt
     8581 atcaaggtat aagaaaaata gtttagttct gtctataatt ttatgttcac ttattatttc
     8641 aatccttgta attactgttt ttaataccgt aaaactacac aatatatcta ataaacaatc
     8701 taaaaactat ttaaatgatg tatctacaca gattgtaatg aatgttgatt ctaaaatcaa
     8761 atttatttta tccgacttaa gaataatggc agattttatt aagcaatatg aaggtgatag
     8821 tcgatatgag tatttatcta aaagaaagac tagttataag tattatgatg ttggaataat
     8881 agatttagaa ggtaaagctg agtttttaag tggaaaaaag tttgacttaa agaatactgt
     8941 ttcctataaa aaaggaattg aaggtaaaga atactcagaa gtaatagagt caatgggttt
     9001 tgttctttat agtgtaccta tatttaatga taatcatgaa gtatctagta ttttaatagg
     9061 tgtatccaat aaagaaagta tgaatgagat tcttaatata gataacttta atggaaaagg
     9121 aactattgaa attattaata gtaagggaaa acctcttttc ataggaaaaa atactgaatt
     9181 aataaaggac cttagccata aatataagag ttctggagat gaaccttggg cacaaaagat
     9241 gcttgaggat tttaaaaatg ataaatcagg gaatattaca ataacctcat caaaaggtgt
     9301 acaatgctta ttaacttatc atccaattac aagaggttta aatgattggc attttcttct
     9361 catagtacct gaagatgctg tacttggtga acttaataaa ttaaactctt ttacaataac
     9421 aatgacattt ataattacat gtattattgg aattataaca ttaattttat atattataag
     9481 aagaaaatat gtaaatcaaa tagaaaatat tgcatattta gatagtataa caaatggtat
     9541 aaattcaact aagtttagta tgctagtcaa acctttaatt tcagaatcac ctgatagtac
     9601 ttatatgatg atagcaatga atataaaaga ttttaagcta ataaatgatt gtttcggtag
     9661 tgaaaaggga aatatgacac taaaacatct ttacaatatt ttgtataaaa acattgatga
     9721 aaatgaagaa tttgtgtgcc gtcatgatgc agaccttttc tatttgctta ttaagaatag
     9781 accagtgtta gaagccctgg agttattata taaaatagaa gctgacacaa attattttaa
     9841 taaaaatagg gaaaatccat actttttaag actatcagta ggtatttaca caattgaaaa
     9901 tcataatgaa gatttaatta caatacaaga ccatgcaaat acagcaagaa aaagctttaa
     9961 taaaactcat caaagtgatt tttcttttta tagtgatata gaaagaaaac gattaatatc
    10021 tgaaaaggaa atttcaaatt taatggaaaa agcccttgaa aataaagaat tttttatgtg
    10081 cttacaacct aaaattgata taaaaagtgg aaaaattagt ggagcagagt ctcttgtgcg
    10141 ttggaaaaat tcagaaaagg gcataattta tccaagtgat tttattcctc ttttcgaaaa
    10201 aagtggattt atatgtaagt tagatttata tatactagaa gaaacatgca aattgatttc
    10261 taagtggata aaagaaggta aagaagtgtt gagaatttct gttaatgtat cacgtcaaca
    10321 tctgaatgat aaattgtttt tagaaaaata taagcatata tgcaataaat acaatgtacc
    10381 aacatgtctg atagatttag aacttaccga atctattttc ttagaaaatc cagaagctat
    10441 agatataata gaggatattc attctaatgg atttaaatgt tctatagacg attttggatt
    10501 tgggtactca tcattaggaa tattaaagga ttttaaagtt gacataatta agttagaccg
    10561 ttcattcttt gttagcaaaa ataatataga ccgtggtaag gtagttatta agtctattat
    10621 tgaacttagc aaaaggctag gtatgaaagt aactgctgaa ggaattgaag aattagaaca
    10681 agttgagttt ctaaaaaatg tgggatgtga ttatattcaa ggttatgttt tttcaaaacc
    10741 attattaatt tctgattttg aaaattttgc atatgaaaat aataaaatta aaaatatcca
    10801 ttattaaatg tacaagataa aaaatttagg caagttatat aattaaaaat ataacttgtc
    10861 ttttttattt atatatttaa taaaaatata tttgcagatt ttgtaaaaat aattttcaaa
    10921 tgttgtaagt tgtggctaaa gctttattgt attattctga atatagaaag atagttactg
    10981 tcttagataa aaactgaaac atacataata atataaaatt tgctatatta ataactctaa
    11041 ttattttgga aggagggaaa atatgataag tgggagaatg ttactcatca tgaattttct
    11101 aaaaggaaaa aataagacaa gttacaagga tattagcaaa caacttagta tagaagaaag
    11161 aaaagttaga tatgatataa ataatttaaa tattgttttg cgttcattaa acaagtcagt
    11221 aatacaaaag atgaacaaag gtgtgttaat tattcctgat gacttccaag tagtatgtat
    11281 aaataataac gaatatgtat tttccttaac tgagcgagta tctattatga aaattatatc
    11341 aatgtttaaa atagataatc taaacttaga aaaattaagt aaagaatttc aggtttctag
    11401 aagttcaatt aaaaatgacc ttaatgtttt gtcaagtgaa ttagaaagaa accaaataac
    11461 tattacttat gataaatctt ttaaaataag tggagaagaa gacattatat ataagcaaag
    11521 attgaacata ttaaaatcgt attcttatct ttttggtaag gaaaaatctg atttaagtgt
    11581 atttgagaag tatttagtga atgtaattga aaaaatattc aaagggattt atttgattga
    11641 tatatataat tggtctatga atttattatt gcaaatggga tggactttaa atgatgaatc
    11701 ttttacatgg tatgtgtcaa acattttcct ttttacatgg tatatacatt cagatgaaaa
    11761 acatccttta gaatctgctt cattaagtga gccgtgtttt gaaaataagt ttgaccaaga
    11821 atttgaaaca attattcaaa aaaagttgac taaggaacaa atttttctgt taaagagttt
    11881 tgtatttttt acaaataaat atgcaagttt aaacgaggat atggatttga ttagtacaga
    11941 aattatagtt caagatctaa tttacaatat gtcagaaata ttagagatta gctttaaaga
    12001 agatatgatt ctttataaag gattactcaa tcatattgcc ccattgatag agagaatcaa
    12061 acagaatata cagatttatg aggaattact tcatgtaata ccagataaat atcaatatgt
    12121 acttgaagct actaaggttt ctattcaaaa aaacagagtt ttaagtcaaa taaagaatga
    12181 aaatgaaaca atacttttgg caatacattt cttaggaagt atacatagaa atgagaggaa
    12241 taactacaaa aatatattgt tagtttgtgg atttggatat ggtgtaattg ctatgttaaa
    12301 ggatagttta gtaaatgatt accagataaa tatagtagag agtattccat catataagtt
    12361 acaagattat aaagaatggg aaagtattga cttcattata acaacatcaa agatagtaat
    12421 agacttgcct aaaacaatta ttgagataaa tccattttta caagaagagg attatttgaa
    12481 gtttgaagaa aaagggataa aaagaaagaa cgtcttaact aattatatgt caataaaaaa
    12541 aaggttggac tttttagaag aagatgccca aaaaagagta ttaagtatta tatgtcaaga
    12601 acttggatac agtgatgaga gaatattatt tagacaactt aatttaagtg atttaatagg
    12661 aattgatacc ataaggataa ttgatggaga tatgaaatgg gaagatgctg taaaaattag
    12721 ttcaaacata cttgctaatt gtgattttgt atcagcacaa tatgctgaga atattataga
    12781 tattttaaaa aatgtgggtt tttatgctgt aaaagataac gaatttgctt tactacatgg
    12841 caatgatagt tcattagtca aagtaagttc gataagttta ttaatatgta aagaagcagt
    12901 tagttttgga gataaaaagg ttaaaatcat attttgccta gcaagcagag ataaaaaaga
    12961 gcaaattcct gcaataatta atctaactaa aatggtttat aagactaatt ttattgctat
    13021 gttagaaagt gcaaagacaa aagaagaagc agaagtttta attcataaat atgagaagga
    13081 ggtcactgta tgaataaatt agttaatgta aaatgttcat ttaaaataca tgttaattca
    13141 aaagaagaag ccattatatc gttagttgat gtagtgtcaa aagaaggata tttaatagat
    13201 aaaaatcaat ttttaaaaga tgttttaaag agagaagaaa cattgtcgac ttatattgga
    13261 catggtatag gtcttcctca tagtcaaagt gtaggtgtta aaaactcttg tattacaata
    13321 ggaaagcttg atactcctat tgaatggaca gaagaaggag aaaaagtgga tttaatattt
    13381 ttgatatcag taacaaaaga caatgaaaat aatcttcatt taaagatttt atcaaaatta
    13441 gcacgtttat tgatgcatga aagttttaga aatcaaatta gatgttcaga tgaacaaact
    13501 gtttataatc ttataaaaga aaaaatagag gaggaagatt aaaatgagaa ttttaaagtt
    13561 tttgcaaaaa catcttatga cagcaacatc ttatatgatt ccatttgtgg ttgcaggagg
    13621 aatattattt gctttatcag taactttaag tggacaagct gctgttcctg aaacaggctg
    13681 gttggctaaa ctgaatcaaa ttggagctgc tggcttggct ttattcattc ctatattagg
    13741 tggatatata gcatttagta tggcagataa accaggatta gctccaggta tgataggtgc
    13801 atatttagca aaagaagtaa atgcaggttt tataggtggt atcatagcag gatttatagc
    13861 aggatttgtt gttttacaat taaagaaaat taaattagct cctacaatga gaactttagg
    13921 ttctatattt atttatcctt tagttggtac attgattaca ggtggtataa ttgtattttt
    13981 aattggagaa cctatagctt cttttatgac atggatgaca aactggttaa atggaatgtc
    14041 tggtgtttct aaaattcctt taggtggaat cttaggagga atgatagcct cagatatggg
    14101 tggccctata aataaggttg ctgctacttt tgcccaaact caagttgaca cacttccata
    14161 tttaatgggt ggagttggcg ttgcaatttg tatacctcca attggtttag gattagctac
    14221 attattgttt ccaaaaaaat ttagcaagga agaaagagat tcaggtaaag cttcactatt
    14281 aatgggatgt gttggaatta cagaaggagc tattccattt gcaacatcag atccagttag
    14341 agtaatacct tgtattatgg ctgggtcgat tgtaggaaat attatggctt ttttattagg
    14401 atgtttaaat catgcaccct ggggaggtct gatagtgtta cctgtagttg acaataggtt
    14461 ggggtatatt gcatcagtat taactggagc agtagttgtt gcggtattaa tgaaattagt
    14521 aaaaaaagac gtgaaagaag atgaagaaat tgaagaagat ttagatgaat caattgaatt
    14581 aatatttgaa gaattataaa tataaatgga aggaatttgt ataaaaaagt aaatataaaa
    14641 ctataaattt aaaggagata agaatatgaa aattgtagca gtaacatctt gtcctagtgg
    14701 agtagcacat acttacatgt cggctgaatc acttgaatta tcagccaaaa aatttggtat
    14761 agaaataaag gttgagactc aaggaagctc tggaatagat aatgaattgt cacttaaaga
    14821 tattgatgaa gcaacttgtg taatattgac taatgatgtt gagattagaa atatggaacg
    14881 ttttaaagga aagaaggtag taagaatgag tgtatctgag ataattaaga aatctgatgc
    14941 attaattaag aaaataaaag atacttttca ataaaaattg atttatttta catataactc
    15001 aaagatgttt ttaaagaaat ttttattaaa agcatctttg aaaatatctt aaaaatagaa
    15061 taaaaggaga tatagtcatg aaaaagttac caataaagac tgtcgtgcaa agattactaa
    15121 acttacaaga agaggggaaa agtgcaactc ttttaggaat tggtcctatg tcaccaaact
    15181 tgttacaagc aagttttgaa ctagcaaaag atgatgattt tcctttaatg tttatcgcaa
    15241 gtagaaatca agtagatgca gatgaactag gtggcggtta tgtaaatggt tggaatcagg
    15301 aaacttttac aaaagatatt aagaaggttg ccgacaaagt tggatttgat ggtttatatt
    15361 atttatgtag agaccatggt gggccatggc aaagagataa agagagaaat gaccatttgc
    15421 ctgttgatga agcaatggag ttaggaaaaa aatcttattt agcggacatt gaagcaggtt
    15481 ttgacctatt gatgattgac cctacaaaag acccatttga aattggaaaa gtaattccac
    15541 tagatgtagt actagaaaga actgtagaat taattgagta ttgtgaaaac gagagaaaaa
    15601 gacttaatct accagacata ggttatgagg ttggtacaga ggaaaccaat ggaggtctga
    15661 cttcaactga gacgtatgaa acatttataa ctagactaaa ggttgaactc gataatagag
    15721 gtttgccaat gccaacattt atagttggtc aaactggtac tttaactaga aagacagaac
    15781 aagtaggaac ttttaatttt agaaatgctt atgatttagc tcaaatggct aagaaatatg
    15841 gagttggttt aaaagagcat aatggcgatt acttagatga tgtaacttta ttagaacata
    15901 taccatctca aattattgct acaaatgtag ctcctcaata tggtactgaa gaaactaggg
    15961 catatttaaa attagcagaa gttgagtgta aattagaaaa agagggctta gttgagaaaa
    16021 cttctaatat cagacatgtt cttcttgtta atgcaattga atgtggacgt tggagaaaat
    16081 gggtagtagg agaacaaaaa aatctaacta ctgaagagat atttaaggat gaagttttat
    16141 ctaatgaaat actagatatt gcaggacatt atacatttaa taatgatgat gttaaaaaag
    16201 aaatagaagt gctttatgat aatttaagta agaataatat tgatggtcaa agatttgttg
    16261 tagaccatat taaacgtcct ttgcgtgatt atgcagaatg ctataactta aaaggtgtta
    16321 ctacgaggat tttaaataaa taataggact tatagtggtg agtattgtta tactaggtat
    16381 ttatagttat aattatactt accactatta aatttaggag gactaatatt ttgattaatt
    16441 taatttttga tgtagatgat actttatata atcaattaac gcctttttac actgcatata
    16501 ataaagtgtt ttcttcaata aaggatattt ctattgaaga tttatatatg agtagtagaa
    16561 agtatagtga tgaggtattt catatgactg aaaatggaga aatgccaatc aaagaaatgc
    16621 atatttatag gataatgaaa gcttttgagg agttgggaaa ttctattaca gaaaaagatg
    16681 cacaaagttt tcaagatgag tatatatatc aacaatcaca aattacacta ataccagaag
    16741 ttgagaggat tttaaatttt tctaaagaaa gaaatattaa tctaggaatt ataactaatg
    16801 gcccatcaaa ccatcaaaga atgaagttaa agcagttaaa tattgaaaac tgggttgata
    16861 aaagtaatat ttttatttct agtgaggttg gattttctaa gccagatact aatattttta
    16921 gagtagctga gaatgttatg aatttagata gagaaaatac atattatgtg ggtgattctt
    16981 atagaaatga tgtattaggt gctaaaaaag ctggttggaa gagtatatgg ttgaaccata
    17041 ggggacatga gttagaagag ttgttttata aacctgattt tgtaattttg gaacataagg
    17101 atttgatatc tttatttata aaaatatgta gttataaaaa tatgtagtaa aaaaatgtta
    17161 ataatagaat tgtata
//
LOCUS       DAYAOX010000077        17030 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.077, whole genome shotgun sequence.
ACCESSION   DAYAOX010000077 DAYAOX010000000
VERSION     DAYAOX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.077"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(124..648)
                     /locus_tag="ACGI57_001918"
     CDS             complement(124..648)
                     /locus_tag="ACGI57_001918"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9716997.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(713..1465)
                     /locus_tag="ACGI57_001919"
     CDS             complement(713..1465)
                     /locus_tag="ACGI57_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFG9716998.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1496..2794)
                     /locus_tag="ACGI57_001920"
     CDS             complement(1496..2794)
                     /locus_tag="ACGI57_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HFG9716999.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2811..5075)
                     /locus_tag="ACGI57_001921"
     CDS             complement(2811..5075)
                     /locus_tag="ACGI57_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HFG9717000.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5330..6148)
                     /locus_tag="ACGI57_001922"
     CDS             complement(5330..6148)
                     /locus_tag="ACGI57_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFG9717001.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6141..6839)
                     /gene="cbiQ"
                     /locus_tag="ACGI57_001923"
     CDS             complement(6141..6839)
                     /gene="cbiQ"
                     /locus_tag="ACGI57_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HFG9717002.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6842..7126)
                     /locus_tag="ACGI57_001924"
     CDS             complement(6842..7126)
                     /locus_tag="ACGI57_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9717003.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7116..7868)
                     /locus_tag="ACGI57_001925"
     CDS             complement(7116..7868)
                     /locus_tag="ACGI57_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFG9717004.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8050..8242)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8374..10146)
                     /locus_tag="ACGI57_001926"
     CDS             complement(8374..10146)
                     /locus_tag="ACGI57_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HFG9717005.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10160..11089)
                     /locus_tag="ACGI57_001927"
     CDS             complement(10160..11089)
                     /locus_tag="ACGI57_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9717006.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11310..11495
                     /locus_tag="ACGI57_001928"
     CDS             11310..11495
                     /locus_tag="ACGI57_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFG9717007.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            11488..12189
                     /locus_tag="ACGI57_001929"
     CDS             11488..12189
                     /locus_tag="ACGI57_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9717008.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12191..13114
                     /locus_tag="ACGI57_001930"
     CDS             12191..13114
                     /locus_tag="ACGI57_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9717009.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13271..14194
                     /locus_tag="ACGI57_001931"
     CDS             13271..14194
                     /locus_tag="ACGI57_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717010.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14187..14924
                     /locus_tag="ACGI57_001932"
     CDS             14187..14924
                     /locus_tag="ACGI57_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717011.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            14935..15708
                     /locus_tag="ACGI57_001933"
     CDS             14935..15708
                     /locus_tag="ACGI57_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717012.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16249..16857)
                     /locus_tag="ACGI57_001934"
     CDS             complement(16249..16857)
                     /locus_tag="ACGI57_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFG9717013.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5488 a         2950 c         2154 g         6438 t
ORIGIN      
        1 aaataaatta aaaagtgaaa tgtatttgtc taaaaacata ataacatttc actttatcct
       61 catttttaag taatatatat ttttaatttt tatttataac taaatctagt ttttatataa
      121 attttagttt ttttctacat ctgagtaatc tattccaaaa gtttctacag taacagattt
      181 cataacttgt ggagtttgtg gcttatcagc tgaatctgtt ctaactgatg ctatttcatc
      241 aactgtatcc ataccttcta tcactctacc aaaacctgca tactgaccat ctaagtgtgg
      301 tgagttttta tgcattatga aaaattgaga accagctgaa ttaggtgcca tagttctagc
      361 cattgatatg actcctcttt catgttttaa attgttagta aatccattac cactaaattc
      421 accttttatt gagtgacctg gaccacccat tcctgtacca tttgggcatc ctccttgtat
      481 cataaatcct ggtattactc tatgaaatat tattccattg taatatcctt catttactaa
      541 tgatacaaag tttttaactg tatttggagc tatatttgga tataactcaa ttttgatttc
      601 ttttccattt tccatttcta tagttactat aggattttta ttttccataa tttattacct
      661 tccttttcat tagttaaatt acataattac atttattata ttatactcta aattaaagtc
      721 tatcaagtat attttttcct atttcatatc cttcttgata aatagtttta caaaactcat
      781 catcaaagtt aagcgtgtct ttaggagcaa attgagtatt aggtctaact actattattt
      841 tatcttcatc atatactttt ttaatatctt ctggcaactc ataattatat tttgtggata
      901 ttaaaataat tttatccaca ttatccccaa ttaatggttc aattaatgca ctcctaatta
      961 aacctccatc taatttgaaa ccatcataaa ttccttcttg cacatctttc acaaattgtt
     1021 ctttaaatcc aagagttgcg tttggatttg atggtaacaa cccactgtat gaaatgctga
     1081 taatggcttc attacgaggc atttttgata cgtcaacaat cttttctgat actactttat
     1141 tatcaacttc aacataatta acataaaagt tcatttcttc tatattacta tcatctagtt
     1201 cccttaggtt gtctatagca ggagaattat ccacagtatt atttactatt tttacatttc
     1261 cattctggat atcttttata tttgtccaca ttttctccaa gttatcaaca ttccctgtgt
     1321 acaaataata tccatttatt gctcctattg atgtccctga aatcgaataa aatttattta
     1381 ttcctccatc atacaaggct ttaactactc ctgcttgata agcccctttt gctcctccac
     1441 cttctaaaca taatcctaat ttcatagtct catctccaaa ttgtttatct tatatttata
     1501 tttcaaaaaa cttactaaat gttttatata tactatagtg gtcctcaaca tttcctaatt
     1561 ctcttataga atgcattgct aaaattggac ttcccacatc cacagatggt atatctatat
     1621 gagtagaaga tattggacct atagtacttc ctcctctttc atctgaccta ttaacaaatt
     1681 cttgatactc aacattagct tctctacata tatttttata aatagctata gaatatccat
     1741 cactagtata tgcctgacta gcatttatct ttataaccgg accttttccc attattggtt
     1801 tattagttgg gtcatgcttt tctggaatgt ttggatgaat agcatgagct aaatctgatg
     1861 atatcataaa agatgaataa atagactcaa agaatccctc tctatcctta cccattgata
     1921 tgcaaattct ctccaatata tttaacaaca tgtttgaatc tgcaccttgt ttagtagagc
     1981 taccaacttc ttcattgtca aatacagcca ttacatttac accatgttga cctttagtat
     2041 taacaagagc attcaaacta gcatgtacca ttgcaagatt atcttgtctc ccaatagata
     2101 taaattcatc attagcccct attaaacttc ctttttcaaa ttcatataaa aataaatcaa
     2161 aatctataat atcgtccata tttacatcta agttagatgc tatttgcttt ataagaaaat
     2221 tatctttttc taatgtatca tttaacatcc caactaaagg taacatatct ttttgcttat
     2281 tatatgagtg accatcattt atacttctat ttaaatgtat tgctagattt ggtattatac
     2341 aaattggctt tttaaagtct ataagccttt catttggttt taatatacta tcacctttta
     2401 aaactactct accagctatc ccaagaggtc tatccaacca tgtacttaat attgctcctc
     2461 catagcattc tgtatttaac ttcaaataag atttttcagc ttctatttct gcatttggtt
     2521 taatactaaa tgtaggagaa tctgaatgag agcctattat tctaaatcca tctctttcta
     2581 taccttctga atttactaca aaagctacta tagctgataa attcttagta acataatact
     2641 ttccaccaac tcttagattc catctttgat tcaaaactaa ttcttcaaat ccatttttat
     2701 ctaagatttc cttagtactc tcgactgcat gaaaagacga cggactatca tatatgaagt
     2761 ctattaagtt tttagcaaag tttatactat ccattcctca tttacctcca ttataaaata
     2821 tttagtgttg tatattcttt atttattttt ttaattccct gttggtcaaa agctatagta
     2881 acatctgtac caattataga aaccactgtc ccaaccccaa atttaggatg atgtacctta
     2941 gaaccaagtt ttatatcatc tggattagtt tctttattag aatcttttat tgtagcatta
     3001 actttatctg caactttaga tttgcttata gtactcatat actttttagt atatttatct
     3061 aacatattat aacttgcttt agaatagctt agttcttttt ttactttgta caatctttct
     3121 atacattctt ctggcagttc ttccataaat cttgaagcta ttgcaacatt tgttttacca
     3181 tataatgttc tcttctctgt caatgacata tatagctctt ctttagctct agttatacca
     3241 acataacaaa gtcttctctc ttcttctatt tgtgaatcac tcatagattt tatagctctt
     3301 gatataggaa ataacccttc ttccatacct gttaaaaaca caacagggaa ctctaaacct
     3361 ttggatgtat gaattgtcat aagcgaaacc ctatcattgt cttcttcatc atttgattca
     3421 gatgtaagtg ctatacttgt taggaatgtc tctaagtctt tattttcata agtatcatca
     3481 ttactacttt caaactccaa agctatagaa ataaattctc tcaaattatc aattctgtct
     3541 tgagcttctt cacctttgcc tgttaaatct ttttcatttt tatttcttat ctctacaagc
     3601 tcgtccatat atcctgtagt atccaagact ttttctataa gtttgctaac aggataaact
     3661 tcttttatag ttctaagagt ccctataata tcaacaaatc cacttatact tgcccttgcc
     3721 ttagtagata tatctgaatt tgtttctata tcaattaata ctgagtatat actttcctgt
     3781 tttaagttag ctctatcttc aattttttct atagttctaa gccctatacc tcttctagga
     3841 acatttataa tcctttttat agatatatca tcttgtggat tttgaataac tcttaaatac
     3901 gccaacaaat cctttatttc ttttctctca tagaattttg taccaccata tatattgtat
     3961 ggaatttgac ttctatttaa agcgtcctct acaggtcttg cctgtgcatt tgctctataa
     4021 agtactgcaa aatccttata aggtctattt tcttttctag ctatttgtgc aattgaatca
     4081 gcaatgaaat cagcctcttc tatttcacta ccagttaatt gtatctttat aagttctcct
     4141 tctttttttt cactccaaag cttttttctt tttctttcta tattattaga tataacttta
     4201 ttagctgcat ctaatataac ttgtgtagac ctataatttt gttccaactt tacaacatgt
     4261 acattgtcat aatctttctc aaattcaagt atgtttctta tatctgcacc tctccatcca
     4321 taaatacttt ggtcatcatc tccaacaaca caaatatttt gatgttctct tgctaataat
     4381 tttatcaatt catattgagc tttacttgta tcttggtact catctaccat tatgtatcta
     4441 aatctacttc tgtaatatgc taacacttca tcattagcct tgaaaagttc tacagtttta
     4501 agtattaaat catcaaagtc aagtgcacta tttcttttta aacggtcttg atatagcgca
     4561 taaatatctg caatcttaac cattctatta tctgccatat tgatatcttt aaactgcttt
     4621 ggagtataaa gtttgtcttt tgctcctgaa attgcactta taactgcttt tggttcaaac
     4681 actttatcac ttaaatttaa ttcttttaga cagtctttaa ccaatgtaac ttggtctgct
     4741 gaatcataaa ttacaaaact tctattgtaa ccaatcttat ttatatcttt tcttagtata
     4801 cgaacacagc aagaatggaa tgtacttatc cacatatcct tagtttcagg acctatattt
     4861 tgttcaactc tctctctcat ttcatttgca gctttgtttg taaatgttat agctaatata
     4921 tttggtgctt gtacaccttt atcttctata agatatgcta ttcttgttgt taatacctta
     4981 gttttacctg aaccagcacc agccagtata agtactggac cttcagtctt ttctactgct
     5041 tctctttgag caggatttaa tgtatctaaa ttcatatttc tcctcctgtt aacagccata
     5101 aatatatttt aatctaaaat atgaattact tcaaattata attatgtatt atattaatag
     5161 attatttcca atatattaca attaaataac atttttccat ataatttatt tcattgttta
     5221 ataaaaaaaa taaccttatg atttggggaa tcataaggca cctttaaggg gtctaggtta
     5281 agggttatta tatataaatc tattttttta aaataggttt agggcttaat tatttatttg
     5341 tattaagttc tctttccttc caatacttat acaaatcttc ttctttctta aataagggta
     5401 aattcatatt tctatgtact tttactaacc atggggattc taggccagct tgtataatat
     5461 tattttcatt tataaacaca ttttctgctt ttccttcatc aatcaaaatc cccttatcca
     5521 atacatatat gtaatcacat atttcataca ttaaattcat atcatgactg gatattacta
     5581 tttttatatt atttttattt aatcccttta ttatatctac aatagaccta gtacttactg
     5641 ggtctaaacc agctgtaggt tcatcaagta atacaatttc attttccatt gctataactg
     5701 atgctattgc cactcttttt ttctgtccat agctaagaaa atgtactggc ctgtctataa
     5761 aatctattcc attaacagct actagagctt tgtttattct ctctttaatt gttttttcat
     5821 ccataccaat gtttctcatt gcaaaggcta tatcatcata tacttttgaa taaaaaattt
     5881 gtttttctgg gtcttggaag acaataccta cattttttct aaggttgtat aaacttctct
     5941 tatcatattt taacttttct tctttaaaaa gaatttctcc agatgtaggc tttaatatac
     6001 ccattaaatt cataaataat gttgattttc ctgaaccatt tgaacctatt atacctataa
     6061 catttccctt tgaaaaatcc atattaatgt ttaatagtgc attggtattt ttttcatatt
     6121 gatacgttaa attatttatt ttaaacatca ttatccccta ctatgtgaaa tttaccatca
     6181 tacaatttta tgtctaatga tatacacata tcatcatacc ttgtcataag tcttttgtac
     6241 agcatacttc caagaatacc taaggattta tatgaagttt ttaaatttat atatccaaat
     6301 cttagctctt gtgacttgta tatttctgaa aattcttcta aaaatatgaa tataaaccta
     6361 tatattaaca ttgataactc gacaaaagtg tcaggtaaat gaagtttttt aaaaaagaat
     6421 attaattgat taaaaggtgt tgtaagtata caaaaataca cacaagtcaa acaagacatc
     6481 gctctaaata acacatgaat agacatgtta atagacgcct ttgatattcc tacattaaaa
     6541 ctaaaaaatt caaaactata taataaatct gctttattaa aagatatatt tacaagagtt
     6601 attcctatac taagaaataa aaagtacatt ggtattctaa gtaattttaa ataatttttt
     6661 aaatctattc cagccacaca aacaaccaaa atactcatta aaatcataat tccacttaaa
     6721 ataaatatat tttgtattac catagaagca atcagaaaaa ttactcctat ggctactttt
     6781 ttgtttggat ttactttact caatctattt gtatatgcat atttatcaat tataagcatg
     6841 attaacttct ctttcctttt tgaagaccta atatgtatcc tattacacca gcccctaaag
     6901 ctgcttgtgc agagaataat aaactttcta tttctccact tggtggctca tatggactgc
     6961 taaaccatgg tttgtaattt ggatttatct tagtaatttc actttcagct tgaccatctg
     7021 ctcctccata ttcagcacct gagttgacaa gtaatgggaa tataataaga gctactacta
     7081 ataaaagtaa taatatgtta gtttttgttt ttgttttagc actcattagc tttaactcct
     7141 ttatcattat tttctagtat gcttttttct ccgttttctg taattaaatt atatacaatt
     7201 acagtaagta taccttctgc tattgctatt ggtatttgag tcataaagaa aattcctaaa
     7261 aatttaattg ctgaagccat taccccacct gatgggtctg ggaaagccag tgctaattgt
     7321 attgaagtta ttgtatatgt ggctaagtct cctattgctg ctgcaagaaa tactggaata
     7381 gcattatttc catctttttt ctttaaagcc ttaaatatta ggaatgatac gattggccct
     7441 attatcgcca ttgaaaaagc attagctcct aaagtagtta ttcctccatg tgctaataac
     7501 aatgcttgga atattaaaac tatagttcca agaacaaaca ttacgcttgg tccaaacatt
     7561 attgctccaa gacctacacc agtaggatgt gaacaacttc ctgttactga aggtattttt
     7621 agtgctgata atacaaacac aaatgctcca cataaagcta ataatacttt ctttttaggg
     7681 tcttgcttta ctattttacc tattgatttt aatccgaata agaaaaatgg tataaatata
     7741 actccccatg ctatagacca ttttactggt aagtaacctt ccattatatg cattgcattt
     7801 gatgtaactg gagttaaaat gattaacata agtgcagcta taacccctaa tttaatattc
     7861 tgtttcatct tagtatccct ctctcccttt ctaataatta aaacataata atacatgtct
     7921 gatttattta ataaaataaa aaagcctaac tcgatggtta ggcttctaat tttccacaat
     7981 ctaaatacaa acacataaaa caaacatatc tgtataatta ttataagaaa ctttctccat
     8041 ccccgaagaa attactttaa tattttaggc aggtctcctg acttagcttc atcctactcc
     8101 ttttccttcc catgagatat aaaaaatatc atcatagtgg ctaacaaaag tttcgtctgc
     8161 atcacagtag cgggggctgt aaaggaattt aacctttttc cctattaatc ttgcttaaag
     8221 aacctataat attatatatt taatttttaa attattctta aatcattttc tatataaatc
     8281 attgtatacc ttttctgtga aaatttctac attttttata aattttattc atttttatat
     8341 ttttatgtat ataaattctt ttttgtaagt ttattaattt ctaattatct tctttaaata
     8401 cattgaaact acatcagaaa atctagttat atctcttgaa tatataaaat catttccata
     8461 tattaatctt tcagcttcaa gttctgattc ttttcctgta aatactccta atactaatat
     8521 attttgcttt ctagcttttc tcacttcatt ggctgtatct ctcaaaccaa ctatacccct
     8581 ataagccttt tctcctctta atgttctctc actacttttt cctatcttta catcatttgg
     8641 tcttccatcg ctgagaacta tcaggatttt attttcttca tctctattta ttagaccact
     8701 acatatagat tttattgcca aaccatctct attattacca gcacaaaaat attcaaatat
     8761 attttcacta ttttttatac tatcatcata atctttaaat ctttttaata ttgtatagtc
     8821 taaaaaacta gaaaatccca tcactctatt tggtatgcca acttctgtta gagcatttgc
     8881 aattatgtaa gcttgaattg ccacatttcc ctgatttcta ctttgagagc cacttgcatc
     8941 caataatata tcaataacgt atttcccttt ttcattttct atatctttat aaaatatttt
     9001 attattatta cttctaccaa ctctccaagc tctattggct aatatagtac cacaatcaga
     9061 atatactcta cttttagcat tttcttctat taaaattctt gacagacttt cttttaatct
     9121 ctttatttgc tgtctataca ttctagcatg ttctctaaat tttcctaaat tattttcttt
     9181 ttgtcttgtt acatatttta gttgaaatac attattacac tcacttctaa gaactccatc
     9241 tgtaaagtgt attctacaac cttcatgaac atttctacaa ttttgtgtct caattttttt
     9301 aatttcacct ttactcaaat aagtttttcc ataataatgt tctatttttg aatatatctt
     9361 atttgcagtg tcttcatcaa catatataac tcggtctgct gaattgctac atttagaatc
     9421 atcaaaatcc cctacacctt ctacaagcat ggatgaagat attgaatcta tatcactttc
     9481 aataatattt tcttcttcat cactataaag ttcttcatac atgaaatcag caaatgtatc
     9541 aaaatttact tctacacttt ctacattatt ttcatcaata agactatttt cttctccatt
     9601 taatatgtat tcaaaataag ttaaatatat aacatctaat gcttttaaaa tctccataat
     9661 atcttgattg gtatctatat ttcttatatc attaactatt cttctagtca tactatctaa
     9721 tgctgggtgc ttgtccatac tataaagtac aaatgtgtat ttaattttat ccataatatt
     9781 atcattgtgt attttggtaa aacgtgaaac aatatcttta taagcattcc ttcttatgtc
     9841 ttctatccct ggcctgtctt taatgacctt ttcttcaact attgtattta atataacttg
     9901 aattagacct agtataacat ctttgttgta tccttttctt attctatttg taagatattt
     9961 tttaactata tcccaatcta catactttct tctagcacct gcaattattg cgaaatatag
    10021 acctatttct tttgaagagt aatctttttc tgatatatct atactttcat tgtaatcacc
    10081 agctattgtc catgataggt tattagctct attttcaaat tcataatcat catatatcca
    10141 tttcataatc tcacctatat taaatatgac ttattggaaa cacatctata ctctcccatt
    10201 tattaggtat tctagtttta attacatctc caaccatttc tttctcatat acatcaaaag
    10261 tctttcctgt aagtcccata ttaattgcaa gagttggttt taatcctctt cttatagtct
    10321 taagagatgc cataagacca cgtaaatcaa tagctttact agatatctct ccattttgag
    10381 acttcaattg taaatctaaa aatattcctg caaagtgtat taatttttct tcatcagcat
    10441 ctgaaaattc attttttaaa atcatcatta acttatcttc ttctatagga ggaatatcta
    10501 ttaccataaa tctagatacc agtgcttcat taagttcttt tgttcctgca tattcataat
    10561 tcatagtccc aataaatctt gttgctggat gtagattgac tctctcatac ccaggtacat
    10621 caattattct tctatagtct aaagctgaat gtaccacaac tatagcatca ttttttgcca
    10681 tattaatttc atcaaacacc ccaaaacctc cattaatagc acactcataa actgagcctc
    10741 ttctaagttt aacttcattg tctataaatg tatctgtacc tataagtgtt gaactgtctg
    10801 tatttatatg aaatgatgta ttccactgag gtctattaaa tatttcccct agattatcag
    10861 caagtaagtt ttttcctgtt gcctttggtc ccgataataa tatattttct tcttctaaaa
    10921 tagctgctat acacatagac aagatatctt taccataaaa ataagtttta gactttgtta
    10981 ctctgtattc taaatctttt tctaaaccat aataatttct aaaatacagt acatcttcaa
    11041 taagtttttc atctacccct tgcaccctta gattatctat tatatccata tttttacccc
    11101 ttttatataa atagtatttg tttcatttct tatcaccatt ttagcataaa aggatataag
    11161 ctaaaattga ttttttaatc tccccatttt aagtaaaacc atgcaccatt ttatcactac
    11221 aaaattaaga gtactactta ctgatatttc ttgattttgt tcgtctatac gtatataata
    11281 tatgctgata aattatgagg aaaggatgat tgaaatatgg atgcccagaa gtagcaaaaa
    11341 aatggggtat ttctgaaaga agagtacaaa aactatgtga agaaagtcgc ataccaggag
    11401 tttcaaaact cgggtatatg tggctgatac caaagaacgc agaaaagcct gttgatggta
    11461 gaacaaaaag aagaaaggaa gtacaatatg aatagcgttt tgattataga tgatgacaaa
    11521 gaactttgtg ctcttatgaa aaaatgtgtt gagcaagaaa atttatctgc tattactgca
    11581 cattgtggtg cagaaggtct acatttggtc gataaaaata gacacacact ctctcttgta
    11641 attttggaca taatgatgcc agatatagac ggttttcaag ttctgaaaaa aattagagaa
    11701 acaagtaata ttccagtaat tatgcttact gctaaaaatg acgaggatga taaagtttcc
    11761 ggactacgtt taggtgctga cgattaccta acaaaaccgt tcagcataaa cgaacttatg
    11821 gctcgtgtca attctcttat tcgtcggtat actatgttaa accctgcttt tataacagac
    11881 acagattgca taataataaa tggcatgatt attgacaaga caaaccgaat tgtttctgtc
    11941 aataatattc ctgttcaact tacaagcaaa gaatttgatt tacttgcttt tctggcttct
    12001 aataagggac gggtgtttac caaaaaacag atttatacac aagtatggga agaagaatat
    12061 gcttttgacg atagtaacat catgtctttc atcagtaaat tgagaaaaaa gattgaacca
    12121 gaccccaacc atccatttta tattttgaca gttcgtggtg tcggttatcg ttttaataag
    12181 gaggcttaac atgaatacaa gtgtctatct gcttttgata ttggttctta gcctgattat
    12241 tatagcgtac cttgtttctg tcctgctacg tgtccgaata caacttacac ttataaaaga
    12301 tgctttagag gatataaaaa atggaaacct taatcgacgt attttaacag atgaaaacga
    12361 cattacaaaa caaatttgct atgatattaa cgaaatagca atcaatagcc aatttcaact
    12421 tattcagcaa aaacaatcag aacaagccta caaacagcta atgacaagtc tctcccatga
    12481 tgtaaaaact ccacttgctt ctttggtcgg ttatttggaa gcagtagaaa gtaaaattgt
    12541 tgtaggagag gaaaaagacg agtatatcca tgttgcgtct aataaggctc attacttaaa
    12601 gaattttgta gaaaatctat ttgaatgggt aaaattggat tctaaggaac aagtttttca
    12661 ttttgatatt tttgacttga atgaattatc ccgtaatatt atatctgatt ggatacctgt
    12721 tttagaaagc agtcattttg aatatgaatt tgatatacca gaaatagagt acttcttacg
    12781 tatagacgca aatgcatata cccgtatcat taataaccta ttacaaaata ttattaccca
    12841 tagtagtggc aacaaaatga cattgagaat ttttgaaaac aaagagcaag ctcaaattat
    12901 aataacagat aatgggaaag gtatatcttc agacaatctc ccacatattt ttgaaaggtt
    12961 gtaccaatgc gaccattcac gagcttcaaa gggaaatggt ttaggcttgg cgattgcaaa
    13021 ggaacttatt aacgctcata agggaaatat taccgccaac agcactcccg gtatgggaac
    13081 agaatttaca atattgcttc ctaaagccct gtaaaagtac gaggctttct tactctaaaa
    13141 caagataaaa gcaagattat ggcaaggttt tggcaaggtt catgttttat aattctaatt
    13201 gtagttgatt ttgacgttgt atcaactatc ttttgtagaa acaaatttat taaaagtgag
    13261 gaaattatat atgaatgatt ttgtgattga aacaaagcaa cttacaaaaa tttatgggaa
    13321 tcaaacagta gttgacaaag tcaatctcca tgcaaaaaaa ggccgaatat atggtctgtt
    13381 aggacgcaac ggagctggga aaaccacaat tatgaaaatg attttaggat tgacttcaat
    13441 cacgtatggc gaggtagatg tatttggaga gaatattaag ggacgtgaaa aacaagtata
    13501 tccccgtatc ggtgcaatta tcgaaacgcc aggattttat cctaatctga caggaacgga
    13561 gaacttggag atttttgcaa aactgcgtgg tacagccgcc cctaattctg taaaaagagc
    13621 attggaaatt gtaggcttac cttataaaaa taaaaagctg tttagtgaat attcacttgg
    13681 catgaaacag cgacttggta ttgccaacgc tatcctgcat gacccagaac tgttagtttt
    13741 agatgagcca accaatggtc ttgaccctat tggtattgcg gagatgagaa agtttattag
    13801 ggacttgagc gttgagcatg gaaagacaat tcttatttcc agtcatattc tttcagaaat
    13861 ctcattgttg gctgatgata ttggaattat cgaccacggt gttcttttgg aagaaagtag
    13921 tatgagtgaa ctagaaaaga aaaacagtaa atatatcctg ctacaagttt cagatattcc
    13981 gaagacatca cttattttag agcgtcaatt taacacgaag gattattccg ttcaagatga
    14041 ctacacgtta cacctttata acactaatct ggatatgggt gaaatcaata aagcacttgt
    14101 aatgcagaat gtaactgtta ttagttctca actttgtaat gatactttag aggactactt
    14161 taaaaaaatc acaggaggag agggcattgc ttaaacttat tcaaacagaa tttcttaaac
    14221 tgcgtcgtag gaaacttatt tggctgatgt tgttgtctgc tctcattatg ccgtttttcg
    14281 cactactata cttcaattat tttggtaaaa caggtgttga gccaataaag ttttataaat
    14341 ggtctgcatt tggttataca ctttggatta ttttgcctgt tgttttgggt atattttgca
    14401 ccatgcttat acacgaagaa aaccagtatg atatgctcaa acagctttgg attgtaccta
    14461 ttagtaaaat gggatacttt tttagtaaat ttttcgttgt tttgatttac tctatttgtt
    14521 ttatgctgat tacagccgtt gcttctgtgt tatttagtgt actttctgga tatgtcgtat
    14581 ttagctgggc aagcatttta tatttgttga aaaaatgttt agaaattggt gtaatcactg
    14641 cttttgtaat gttgccgatt ttggcaatcg ctacatctca aaaaggatac attctcccaa
    14701 tttgtatcac attggtatat gccttttccg gttttgttat aatgacaata aatatgtatt
    14761 tacacccatt atccagtatg ggagttatta tcatgagaaa taaagacatt ccaggtctta
    14821 tattttcaca ggaaattaat gtcccattag catttctttg tatttgtatt tggagtattg
    14881 tatcagtatt gtttgcaaac actgcactag gaagaagaaa gtgaggtgta gataatgtgg
    14941 aaattacttt gggctgaatg tcaaaaaatt cgtcgttcaa aaattgtttg gattacagtt
    15001 tttgcatcca ttatgatagc agtcatagtt tttgcaggag ggcaggatgt atataatgac
    15061 ccagatttac attatggctt aaagagcgtt tatgacggtt cacggtatat agacaatgca
    15121 ggttggtata tggacgcagt acaaccttgg tcaacttttt ttgttcttcc tgctgtcatt
    15181 gccctattag gaagctatat tatttgtcgt gaagaagagg aggacactat taaatccctg
    15241 cgtcttattc ccataaatga aacaaaatta accatagcaa aaatgattat tacttttata
    15301 ttcagtatac ttctttattt gttactcttt actataacat ttttgactga atccgtttta
    15361 catttttccg atttatcgac aaaactggtt ttaagttgca tgaaagagta ttttcttgat
    15421 ggcataggtg tattctttgc aatttctccc attattgcct tggtatcgcg catgaaaaaa
    15481 ggatattggc ttgcattagt attcacagaa gtttattcta ttgctggact atttgcgggt
    15541 atgtcaaatg tcttacaaac cttttttcct attgcggcaa tatttaatat ttctggatac
    15601 tatataacta ctggagaaaa aattatgggg agtatcataa gtttattact ttgtgcctgc
    15661 ctttctgcat ttatacttaa agggctaaaa cacaatgaaa aaaattgaaa taggtatatc
    15721 aaaatgaaaa agataaaaaa actgattatt ctaattgtat tcttgtttct ttttggcatg
    15781 ttacactgtt atactgaatg gaaattcaac aataacccac ctaaaggggt aatagaaaga
    15841 ataaatgaat ggactgataa gattggactg gattagtaac ttatgttaat tatagtgaat
    15901 gtaatattag caagttattg tttcgtattt taatactata actttaaata tttggtttta
    15961 atcttccaaa cgaatataaa aaaactgtca cagaggcaac atatttttga atatttataa
    16021 agtggtagtt ttgattttca aagctgccgc cttttcttct atataagcaa acttatcctc
    16081 aacaaagtgt aaaacatggt acattttaag aaaaaagtac cacagacaca agcaataaaa
    16141 gaaacgccga gaacctacaa aaacgtagtg cttatgcaga tttacgacgc cttctatttt
    16201 ctcaaccgtc ttaatgacct tgtacaaaag aaatttttct aatatttttt ataatcttat
    16261 cttgtgatgt tttttatgta taaatgactt aataacaaat ccaataaaaa ttatggatat
    16321 tggtataatt agaacgagga atttataatc attataatgt gacattattc ttacccaact
    16381 ctcagaaaaa atatatccta tactgattaa tgaagtattc catatggtta ttcccacgat
    16441 agatgatatt acaaaagtta taatattttg acctgctata cctgctataa aagatatgta
    16501 tgttctacat aaagggatta atctacatac acaaacagaa atagaagagt atttgttaaa
    16561 ataatcttga gatgcaaata ttcctttctt tgcctttgga catatctcta ttattttatt
    16621 tatgatagat ttacctcccc aatttcccac tgtataacaa aatatcgaac caagtatacc
    16681 tgcaattata cttaaaatta aaattgtcag aaatccaaaa tgatttcttg cagcaattgc
    16741 tccacataaa ggtaaaacaa cttcgctagg taatggaaaa caagcatact caatcataat
    16801 taatacaaat acagagatta atccatattg taaaataaaa ctattgatta cagacactta
    16861 cttatctatc tcctttctta tttaaaggtt ttaagggtac atgttctaag gattctcacc
    16921 ctatatttaa agaatcaatc tctttaaact attatatgta tttacacaaa gcatttataa
    16981 catactttaa aaatagtacc ttaaaataag aaacttaata aataaaaaaa
//
LOCUS       DAYAOX010000078        17014 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.078, whole genome shotgun sequence.
ACCESSION   DAYAOX010000078 DAYAOX010000000
VERSION     DAYAOX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.078"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(370..1092)
                     /locus_tag="ACGI57_001935"
     CDS             complement(370..1092)
                     /locus_tag="ACGI57_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFG9717014.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(1151..1828)
                     /locus_tag="ACGI57_001936"
     CDS             complement(1151..1828)
                     /locus_tag="ACGI57_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFG9717015.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1856..2671)
                     /locus_tag="ACGI57_001937"
     CDS             complement(1856..2671)
                     /locus_tag="ACGI57_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9717016.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2897..3107)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3825..5891)
                     /locus_tag="ACGI57_001938"
     CDS             complement(3825..5891)
                     /locus_tag="ACGI57_001938"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFG9717017.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(5968..8997)
                     /locus_tag="ACGI57_001939"
     CDS             complement(5968..8997)
                     /locus_tag="ACGI57_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9717018.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(9050..10510)
                     /locus_tag="ACGI57_001940"
     CDS             complement(9050..10510)
                     /locus_tag="ACGI57_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFG9717019.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(10602..10976)
                     /locus_tag="ACGI57_001941"
     CDS             complement(10602..10976)
                     /locus_tag="ACGI57_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717020.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(11152..11892)
                     /locus_tag="ACGI57_001942"
     CDS             complement(11152..11892)
                     /locus_tag="ACGI57_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFG9717021.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(11913..12701)
                     /locus_tag="ACGI57_001943"
     CDS             complement(11913..12701)
                     /locus_tag="ACGI57_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HFG9717022.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(12789..12879)
                     /locus_tag="ACGI57_001944"
     tRNA            complement(12789..12879)
                     /locus_tag="ACGI57_001944"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12843..12845),aa:Ser,seq:gct)
     gene            13138..13902
                     /locus_tag="ACGI57_001945"
     CDS             13138..13902
                     /locus_tag="ACGI57_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFG9717023.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(14183..15298)
                     /locus_tag="ACGI57_001946"
     CDS             complement(14183..15298)
                     /locus_tag="ACGI57_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFG9717024.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(15339..16865)
                     /gene="glpK"
                     /locus_tag="ACGI57_001947"
     CDS             complement(15339..16865)
                     /gene="glpK"
                     /locus_tag="ACGI57_001947"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFG9717025.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
BASE COUNT         5412 a         3094 c         1750 g         6758 t
ORIGIN      
        1 ataaaagtta aatttataag cttacttgtt ataaaaataa cttttataat ttatttcaaa
       61 aaaaatattg atttttgtgt cgcattttgt tataataaat taaggttatc cccctggtaa
      121 ccctagttag ttaatatatc tattcccaat accctttcat aaggatatat tttatattta
      181 ttaaaaatcg gattagtaac ggaaattaat tcggtggaaa aaaagagctt accccgagct
      241 cttttttttc tgcaaaaaat cgtaaaaata aagttaatat taatataact ttaattttaa
      301 aattaatatt aaccatcact aaaaaaggat gtattgaatt tctaatatca ataacatcct
      361 ttttatatat tatagcactt ttgctaaaaa tgcttttgtt ctttcatgtt ttggatttcc
      421 gaaaacttct tctggagtat tatcttcaag tatacttcct ccatctataa aaataactct
      481 atctgcaact tccttagcaa atcccatctc atgagttaca attgccattg tcataccttc
      541 tttagcaagt tcttttataa catccaaaac ttcgtttacc atttctgggt caagagcaga
      601 tgttggctca tcaaacaaca tcatatctgg ttccatagca agtgctctag ctattgctat
      661 tctttgcttt tgtcctccag ataattgtga tggatatgaa tcttttttat ctaaaagacc
      721 aactctactc aacaaaattt ctgctttttt ttctgcttct tccttagtca tcttttttaa
      781 tttaattggt gctaatgtta tattatctaa aatagtctta tgtgggaata aattaaagtt
      841 ctgaaacacc atacctatat tttgtctaac ctcatcaata ttagtttttt tatcgaccaa
      901 attttttcct tcaaatatta tttctccacc tgtaggtatt tctagtaaat tcatacatct
      961 aagaagtgta cttttaccag aaccacttgg acctactata accattattt cgcctttagc
     1021 gatttctaag tctatatttt ttaatacatt taaatctcca aaagatttac ttaaattttt
     1081 tattgtaatc atattatcac tttccttttt actattctat gttttcccta aatgctaaac
     1141 atgctctcat ctagttagac acagatagtt tcttttctaa taatccaact aatcttgata
     1201 gagtaaatgt tataacaaag taaattgctg ctgctactat taatggctct aatgctctaa
     1261 atgctgcatt ttttactata tcagcagtac gcattatctc aacaactcct attacagata
     1321 ttattgaaga ttcttttaca agtgtaataa actcatttgc taatgaaggt aatatatttt
     1381 ttatagcttg aggtattatt atatatctca tagttgacca ataatttaat cctaatgatc
     1441 tacttgcttc catttgtcca ttatcaactg attgtatacc tgatctaagt atctctgcca
     1501 cgtatgcacc agagtttact gataatgcaa atgcactagc tataaattca cttggtatac
     1561 caaacaacat agggaatctt attcctaatt gaggtaaacc aaaccatact atatatatct
     1621 gtactaataa tggtgtccct cttaaaacct caatatagat tgatgatatt gctcttaata
     1681 ccttgctctt tgatattttt gccatacaga taattatacc tattataaat ccaaagcata
     1741 atgccacaag agaaattgat actgtcacac ctgttccttc caaaaaagaa gtcccaaatc
     1801 tacttaaaaa actaaaatct aaactcataa tttcatctcc cacataactt tcttactaat
     1861 ttgaagcagc ttcagtagat gcttcattta aaaattcttc tatcttcttg ccatcttgta
     1921 attcttttat ctttttgttt aataattcta taaaatcttt gttattgtca gattttttga
     1981 ttgctgctgc cataccttct gttacatctt tcccaacttc agtatttgcc atttttattc
     2041 catcatattt tttaacattt attaaagaaa ctgcttcatt agtaacaact gcatctatgt
     2101 ttccattttt taattctgta attaaatcag gtattgcttt caaagatttt gtagttgtca
     2161 tttttaaagt gttaactaca tattcttctt ggatacttcc tgcttgaact ccaactctaa
     2221 gtttctttaa atcatcttca gtttttactt tgtctatatc tgcatcttta actataactg
     2281 catttctact taaataatat aattctgaaa aatcaacact cttttctctc tctggtgttg
     2341 gagacatacc tgcaagaaca atatcaactt ggtctgcatt taaagcacct attaatccat
     2401 caaatgacat atccttaatt tcaaccttta caccaagctc ttttgcaaat tcttcagcca
     2461 acattatatc gaatcctttt attgaatctt ttccatctac taccttatga aattcaaatg
     2521 gaggaaattc tgcactagta ccaacaacta attttccatt gtcttttatt tgttctaact
     2581 ttgttttttc tcctccacca ctacatccta ctagcgataa tgtcaatcct aatactaatc
     2641 ctaaacttaa taatttttta aatactttca ttttaactac ccctctcttt taattataat
     2701 ttccctcaga ttttaatact taaatgtttc gagttacaaa aaaagtctct cagtctttaa
     2761 tgactaagag acgactttac cgcgttacca ctcttattga taaatactta tattactttc
     2821 aaaatttgtg actgttagca tttaagctgt atggatactt ttatttttaa atcaaaaaag
     2881 tcccttagct ttacagctaa gagacgaatt atatccgcgt taccactctt tttgataagt
     2941 aagtacttat cctctcaatc ccttaaggcg ggtaaaccaa ttatcatact aaagtttctg
     3001 ataatctcct ccagagctct cttcacatag acttcattgc taggcttaca ccaaccccta
     3061 actcgctgta aatccatata tgctactttc tctatcattg gatttgtttc cttaaattgt
     3121 taatacaatt gtacttcctt ataatatttt tgtcaataag ttttttattt ttttaataat
     3181 ttttacttgt aaattatcta aatactactg tttatattta ttgatattag ttttgtaatt
     3241 ataaatagta tatttcatat ttttagatat ttcttaaata atactaaata atatctttca
     3301 cattgttgct ttttcttaaa caatactaaa taatatcttt cacattgttg cttttcttaa
     3361 attacactaa ataatatctt tcacactgtt gctttttctt taataatact aaatagtatc
     3421 tttcacactg ttgccctttc tttaataata ctaaataata tctttcacat tgttgctttt
     3481 tcttaaatta cactaaataa tatctttcac actgttgctt tttctttaat aatactaaat
     3541 agtatctttc acactgtttc tctttcttta ataatactaa ataatatctt tcacattgtt
     3601 gctttttctt aaattacact aaataatatc tttcacattg ttgcttttct ttaataatac
     3661 taaataatat tttccacatt gttgcttttt ctaaacaact ataaataata tattaaaccc
     3721 ttatttcttt ttctaataag ttaaataata cattttacac atatctattt gtcttgcaca
     3781 taataaataa tacattatat ttctatctaa atttagattc taaattaaat agtataaatt
     3841 atattcttca ataaacaagt tttcaaatcc aagattcgct ctctatcttt aaactttatt
     3901 gtaaacataa ttccatcttt tttaattatt tctccttcat gaaatttttt atggtatacc
     3961 ttatccccta caccaatact gtcaatttct tttttcgttg gagattttat atcattaata
     4021 aataatgatt tatcaacttt cttaccatac ttatttaaag tagagcttat acataacttt
     4081 tcttcagctc ttgtaatagc aacatacata agtcttcttt cttcttctaa ttgttcttct
     4141 tttttctctt ccttacaaat atcatatgat ttttcatgag gtattgttcc ttcatttaca
     4201 cctataatat aaacatttct aaattctaat ccttttgcac tatgcatagt tgtaaatatg
     4261 acaccctcag tctgcttatt cttgttattt tccgttaact cagtctttac tctatcaata
     4321 tgctctaaaa actctgtaac agtattaaaa ttagttgctg aactctcaag ttcatttaat
     4381 atctctacaa gtccattagt ctttatcttt ctgttgctac aataatctaa aacatatcta
     4441 tcataatcaa gactagttct aatataagat attgcatttt taggatttaa agtatttaaa
     4501 taacttaaat ctatttccaa atcatttatt gttttaactt gtttaggatg aagattacac
     4561 ttatttatta atgctgttat aaagtctttt tcatccttaa ccatatttat actgtctttg
     4621 gatatatatc taaaaggctt gtttataatt ctcaaccaat ctttatttga ttttggatta
     4681 atacctattc ttaaataggc tagtatgtct tgacttgccc aatggtcata tattgtaatt
     4741 actgagtctt ttactacaaa tggaatcctc atatccataa atacatctac taatgctctt
     4801 gactgtatat tagttctata aattactgca aaatctgaat actctacata atctttttgt
     4861 atttcgtcaa ttatttccct ggcaatatat aaggcttctt cctctgaatc atgcggagaa
     4921 atataggtaa cacttcctcc atccccctga ctacacttaa tgattttttc atatctattt
     4981 ttatttttgt cgataagtct atttgcagta tgaactatct cacttttaga cctgtaattt
     5041 atatctaata ttattttttt agtattatta aagtattgtt caaattcaag caaaaaatct
     5101 ggtctagcac ctctaaatcc atatatactt tggtcttcat ctccaactgc aaatatattg
     5161 tttaatgggc tacaaattaa ctttaaaact tcaaactgta ctttatttat atcttgaaat
     5221 tcatctatca atatatattt atacacattt ctaaccattt ctagtgcaga cttattgttc
     5281 aaaagtaaat aataagttct tataagcata tcatcaaaat ctattttatt aactttagat
     5341 ttttgttctt cgtataagtt gtatactttt aaaaactcat cttttgttaa tacttctgaa
     5401 ttaaattcat ttttatccat aagttcattt tttacatagg aaatttcatt tataacttgt
     5461 cctatattct catcatcatc agcattctct atatttaaac tttttaggat tgccttgatt
     5521 gtcattcgct ttgacttttc atcaaatata ctgtctaagt tgtatctttc aaagtatctt
     5581 agaattttaa aaaatacaga gtgaaaagta ccataggtta ccttattaag tcttatgtca
     5641 tcacttaaat tcaaagctct atttttcatt tctattgaag atgccttggt aaaacttatt
     5701 gccaagattc ttgttggtgc tatgtttttg tttacaacca tattggctat tctatatgta
     5761 attactctgg tcttacctga accaggccct gcaagtatca tgcaaggccc atctatatgc
     5821 tcaactgctt ctaattgatt ttcatttaac tttcttatat ctatatttgt tgtgattatt
     5881 agctccacca ccttgattta taattatatt attttctttt taattataaa ctaaaagttc
     5941 ataaaaataa atttaatttt tcctctatta aaccacatca ttcctatttt catgacatat
     6001 cttgagttct tttttaattt tgtcgcttat atcttcaggt ataaatgatg gtttactaat
     6061 atagtagcct tgcatataat ctactccaag attaattagt gtttccattt caccttttgt
     6121 ttctacacct tctgctatca ctttaatatt tctttcgtta gaatatgaga taagattttt
     6181 taatattttc tgtctgtttt catctttatc aatacctctt acaatagaca tatcaatctt
     6241 tacatagtct ggcattatat ataatagtac agcatctccg ttgtaacctg tcccaaagtc
     6301 atctaaagca atttctatat tccaatggtc tatatatgct ttctttttct caataaaatc
     6361 ttcattaatc ttatcattct ctgttatttc tagtactact ctatttaaat attcaccata
     6421 taaatcttca atttcctcaa aatctacttt tgacaaatta ttattaggaa tagaatttaa
     6481 gaacagtttg caatcgccaa atttttcttt gtatttttta aatgatttaa gtgctttaaa
     6541 ccatgtaata cgctcaattt gatataattt agactgtgaa cgagctaatc taatgacatc
     6601 tattggcgaa ctaaaagttt ttattttagg tctcataagt gcttcatatg caaaaacttt
     6661 acctttacaa tctacaatag gctgaaatgc atactcaact aattgaccat caattaactt
     6721 gtttaattct tctctattac tcaataagaa tgaatcctta ttatatatat taatatcaaa
     6781 ttcactaact tcacccttga ttgtattttt aatttgatac atagcaaaat ctgcgtatct
     6841 cattaattca tcatagttgg ttgagtcatc agggtaccag gaaattcctg ctgatgccct
     6901 tattttaaat tctgttccat catgaagttt taataaggta ttattcatct gtttcttaac
     6961 agaattaata atatctctta tactatcttt actttcatat ccatatataa atacataaaa
     7021 ctcatcacct gaaagtctag atactatacc attccatgat ataaacttct taagtatgtc
     7081 tgcagcacac ctaatatatt catcaccagc atcatgtcca tatgtatcat taatatattt
     7141 tagattatct aaatcccaca taataaatgc tgctatctta agttcatctt tattcttaaa
     7201 tttttcttgt atctgtgaat gaaaagctct cctatttaaa agattcgtta aaatatcaaa
     7261 atctctttca tattctatct tccttttctc aataaattct tttgttacat caacaacaac
     7321 accaagaacc tttagctcat cttcaaagat attcattctt atccacttaa gttttttatc
     7381 tttagattca aacttatata tatatgtatt ttctgtttca taactttctt gcaaatattt
     7441 atctagtccc cttaattttt tcaggaactt atttttttcc atatatccat attcttcaaa
     7501 tttgatttca gtagtaaaca cgctaaaaaa ttcttttgta caaaaaacat tctctgcgtt
     7561 tttgtataac tcaaaagctc caattggcat atctaccatt tcaataatct gtgataattt
     7621 tgacgaagaa tctgcaacat ttgcactgag caactctata gctgaagata attcatctat
     7681 ttcagatata tttatcttat caagatttac tggttttgat ggattactgt ttctaacttt
     7741 gttggctaaa agaacaattg gttttgtaaa taatcttgat gcaatataaa tacctattag
     7801 acctattaat aaagaaattg ctatagatat tataactaat ttctctaccc tttttgaaaa
     7861 tgacaaaaga cttttttctt caataatacc caaaagagcc caacgttcat tttcaaatgg
     7921 agtattggag ttatataaat ctaagtattg tacactagca taaatgttgc cattaccaca
     7981 ttcattatct ttatttttaa tcttatatat atttttatac gattccttct gctctaaatt
     8041 agaatgtaaa ccatcccaaa atttttcttt taatgagtat ccatttgaaa ttacattttc
     8101 aaaggccata tcattatttc tatctacacc taaaatataa ttaccctttt tattcccatt
     8161 tatctctgag tatggaagta attctcttaa ataatttaca cttaaatcta ttccaagtac
     8221 tccatataca gttccatctt cataaataag gggaactgta tatgtcataa gcctatcacc
     8281 tgcatttgtc aataaaaact tacttcccca gtatcctaaa ttagaacttg atacatttgg
     8341 attatcaatt gcagctctaa atggtttata aaaaaaatca tctccatctt gctcattttc
     8401 acttttaaat gaaaacttag gttcccaata actgtccata gatattccaa aacttcttgc
     8461 tatatctgat gaacctctct caattaacaa atcagaatta tcatttggat taaacttagg
     8521 gtctaaatcc cttatataca atcctgtcct acttatttca cctttactat ctattgactt
     8581 atcgttaata tttttgtcac ttaggattat aaaagctcct gtaacagaat tttttctcaa
     8641 aagatatatt aaatcatttg atatgctttt tataatatct ttgttaattt cttcattgtt
     8701 gcctacatcc tcaattaagg catttttttc atttaatact ttttttacag aggaattaat
     8761 tgtagcttcc gtttcacttg tatttgacca tctttgtaac atttcatttt gtatataatt
     8821 tttacgatta attactcttt catttaagat gtcaaacgaa ttatcattta atttcttaat
     8881 agttcctccc cataaaattg tggcacaaaa taaacttgtt tgaattagca taacaataat
     8941 taggggaaat atcatctttt tcattattga attgtttttt cttagattgt tttccatacc
     9001 cattccacct atacctttgt gctcgtcaac acactaactg ccaattcatc tattttttta
     9061 tagcattttc aagctcagcc ttaaaacttt caaaccaagt tttaaaattt ttatcattat
     9121 taaattgtgc tactgcatcc ttcttagaag ttccactttt tactaattca tttattttat
     9181 ttgaatcttc tttagcttta tcaagcattg aatattcaag tatttgtcta gctttagttc
     9241 caccatcaaa tgctttatta gtatataaat tacaactgtt aatctgttca atagatgagg
     9301 ctattgaacc ttcaacttta tcagacaaac tcaaattatc tttcttcata acttctttta
     9361 taacatccaa atcattcgtt tttttcttta ctggtaagta tccagaagca atagagaatt
     9421 ttatattagg ttctttatct gtaaaccatt ttaaaaattc tactgatgca tattcttctt
     9481 ctttagtaga ttttgttaca gccattcctg caccttgttg tacagcatat tttccttctc
     9541 ctttaaaacg tggtgcttga aaaacagatg tttcaatatt ataactctca ttatcattta
     9601 gcattactct atctggaaag tatgctgctc ctgaactaga acctacaaga gctaatatat
     9661 cacctgtttt tgcatcatca gaacgaaact taccataaga agcaaaatat ccatttatat
     9721 atggtacata gaaattatcc catagttttt tcataacttt ttcatcaata tttagcgtaa
     9781 ctttaccatc tttaacagaa aaaatttcta ctcctagttg tttacttcct ataataatat
     9841 aatttgctaa agcatctctt ccaaaaaatg ctttaccatc attaggctct ttcgttaaac
     9901 tatctgtcca ttcataatat ttttttgatg tctctactaa tccttcaatt gtttttatat
     9961 catttatatt tgaatttgta gcttttgcaa atttatccca atcagtttta tttaacatca
    10021 aaatttcagt tgattttgca attgggaaaa gtttaaactc atccttagaa tttattctgc
    10081 cttcttctac atattcgtct atgtattcat ttaattcttc tttacttatg tacttatcca
    10141 tatctactag taaccctaat ttatccattt catatgcagt atctgagtat gctgcaaata
    10201 tatttggtac atcttcagaa ccaacttttt tatttgcaga atctaaaact ttctctgata
    10261 aatcaactac attaccttga ctgaatgctt caacaataat tcctttttca gcacctacag
    10321 tgtcattaaa ttccttaaca agcttatcaa aagccatttt ctgctgtcca ttataataat
    10381 gccaaatttg tattgatgtc ggattttctg ggtctaactt ataattatct tccttcttta
    10441 aactacatcc tgtaagtaat aaaacaatca ttgatgtgat aattaatttt gcaaacttac
    10501 acctattcat aaaatctact ccttcaaatt ataaaattta taatagcatt aaaactgtaa
    10561 cttcactatt tatattaatt atagaacgtt tattgatata tttaaacagg atttacaaca
    10621 tatttaaaag caattaagaa acatactatt acaaatatag aaaataaagc ttttacataa
    10681 tttttatttc taaatgcacc aaatgacacc aatccttgtt gaatagctac tgtaattact
    10741 aaaagtatca ttattatatt aagttgaacc agtttaaata agtaaagcaa tgaaaacact
    10801 actagaagta cacataatac aatactagat ttattaataa tatcatcaat cttatctact
    10861 ttcttagatt gcttattaat tttatttaat ttttctttat ttaaattctc aactttttta
    10921 ttataccttt ctcttgtttt atctttatat ttagtatttt tatttttatt tcccatttat
    10981 tttccatcct ctataaattt tttaattgag cttatacatt atttaaatat tatatattta
    11041 agataccata tattcctaca aaaaggtaac ttactatgta tctatttttg attttcgcat
    11101 aaaaaagcca tctcctttag aattaattct gtattgagac agctaattta actatttatc
    11161 ctttgaaaca ttttctacat tatcatattt actatcaact aaaactatat caactcttct
    11221 attttttgac ctaccttctt cagatgaatt ttctgctatt ggtctaaact caccatatcc
    11281 tacagcagat aatttatctg gtgctatacc tgcattgtca ataagaagtt gaacaacatt
    11341 ggttgctctc ataactgata aatcccaatt tgacttaaac tctgaatttt ttatagacat
    11401 attgtctgta tggccttcta ctctcatgta actattcatt tcatttaaca tcttacctat
    11461 ttgaataatc ttatctctag aattatcctt cacaatagct ttccctgtat caaatagtat
    11521 tgtatcttta aggcttatta ctaatcctct atcttgaact cttaatgata ctgaattagc
    11581 cataccattt tcgtttaaat acttctgaat ttcttcacca atttttttaa acttagcatt
    11641 ttgtaaagta tctaaatcat tacttcctgg ttcaactact ggttctatct tgctattacc
    11701 accttcaata actccagaag aaccaccaaa agcagaattt agagattgag ataattgttt
    11761 atatttctcc gcatcaacat tactcataga atacattatt acaaagaaaa tcataagtaa
    11821 tgttatgagg tctgaataag ttaaaagcca tcgctcattg ttttcttctt tttcatcttc
    11881 atcatacaac ataaacattc catcctttta tattattcag ctctaccacc aatttcttca
    11941 agttcaatta gttcttttga atttaaaaat atttttaatt tttctgtcat taaattagaa
    12001 ttatcaccct caagtatgga taatattgct tctatcacca aaagtctttc acttacttct
    12061 tgttgattta tagccttaag ttttgatgct attggtaacc atattaaatt ggcagaacca
    12121 acaccataca aagtagccaa gaatgcaaca gaaatttgag gtcctaatgc atctggatta
    12181 cttgaaagat tacctaaaat atgtactaat cccataactg tacctattat ccccagtgta
    12241 ggtgcatatc cacctgctga atcaaaaatt gctattcctt ctttatgtcg ttttgatgtt
    12301 atctgtatct gtgattccaa tatatttctt atagtaactg catccactcc atctaccata
    12361 agttcaagcc cttttctaat aaaagggtct aattcatcat tctcacttat ttctttgtca
    12421 atactaagaa gcccctcttt cctagccttt gttgcaattt ctttgaaata gattatattt
    12481 tctatgttat taggcttttt atttttaaaa gctaataaca ttatctttat agacttttta
    12541 aacacaggga atggagtcga agctccaaca gctcctatag ttccaccaaa cacaacaagt
    12601 gctgatgaaa atacaaataa tgctccaaca tgtcccccat caataacaaa agcaactacc
    12661 aaagatgcta tccccactat gaaaaaaatt aaagttgtca tctattccct cctttcttta
    12721 gttatacata tctaatcatt tatgacatta taaaaagact atctttaata aaaatagcct
    12781 ttaataattg gcggagaagg agggattcga accctcgcac cggttaccca gcctaatccc
    12841 ttagcagggg atcctcttga gccacttgag tacttctcca tatctctata ctaataaatt
    12901 atataataaa atcatatttt agtcaattgt tttataaaat ttgagttatt ttgaattatt
    12961 aaacatctat ttttactttt ttgcttaaat gtaagtttta aatatggtaa aattatcttg
    13021 cataacactt ataatattat ttgataaata tacatacatt aacaaatata cctaaactct
    13081 gacttaattg taagttatgc caaaactaaa ataatactag tccaaggagg atattttatg
    13141 ttaccacagg aaagatatga gaagatttta agtattctca aatcagataa tataattaaa
    13201 ataaatgaca tagttcaaat gtttgatata tcaatagaaa ctgcacgaag agatttagcc
    13261 catatggaga gtatcggtct tttaaaaaga gtttatggtg gtgctatacc agttatagca
    13321 agtagcgtag aacttaacta tgttaaaaga aaaaaaataa actcaaacga taaaaaacag
    13381 attgctatta aatgtagtga gtttataaat acaaatgata ctatttttat ggatactgga
    13441 actactgtac ttgaagtttc aaaaaatttg aaagataaaa agaatctaac tgtaataacc
    13501 aactcaattt tagttgcaaa cgaacttatg aatactaata taaaagtata tgtgcttggg
    13561 ggctatctta gagaaactga aggttctttt tctggtccgc ttacactgtc agctattgaa
    13621 caatttaatg ttgataaagc tattttaggc gctggaggta tcactcttga aaatggaatc
    13681 tctgactatc acttagaaga agcacttgtt agaaaaaaaa tgattgaaag gtctagaaaa
    13741 tctatagtag ttgtcgaacc tcctaagttt ggtataaatt catttgcatc agtagctcct
    13801 atagaacata tagatactat tattacctct gctagtatag accataatat aataatagag
    13861 ttttctcaaa aaggaatacc tttagtcata gctgataatt agcttatatg attgattttt
    13921 agttccttga tgtaataaat taaaattaag gagccaaata attatttatt tagctccatt
    13981 tatgtctaaa ctatacttta tattttaatt atgtaactac tttcagttag cttatcattt
    14041 ttagttattt gattgccatt ttatttaatt tataagtttt agttattcga ttatttttta
    14101 attgtctgtc tttttaattt atttttagtt atttaaactc ctactttaaa ataatttaaa
    14161 tttgacttaa gtattgttta ttctacactg catctttctt cattttaata ctttgttctt
    14221 cttccaaggt taaatctttt gtcataagta tagcaagtgc aattgccaat actcctccaa
    14281 ccaattttcc aattacaaca ggtgtaatca tctctggaac tactcctgct gtaaatccta
    14341 aatggtcccc caaagctgct gtagcacaaa ccagccaagc agtatttact actttcccct
    14401 ttggactcat atctttcatc attttatata ctggaatact attggctaaa gtaaaaacta
    14461 atcctgctgc actagtggca tccataccta attttccacc cactattgta agtggtttat
    14521 taagaacttt aactaataaa tgtaatattg gaaaagtacc taataacacg attgcaatac
    14581 ttccaattac ttgaatacct tctcttatag gtgtcatgcc ttttataagt actatacctg
    14641 ttatttcttg aaatgctgct gctcccaaac ctatcgttat caatatagat ataaattttc
    14701 caaaaattaa tgacccattt atcataagag tagaattaaa ttttaaacct actgccaata
    14761 ttactgccaa tattatcact ggtatcatat ttataattac cattgataag ttaaatcctg
    14821 ctattaatcc tcctataata cctccaaagg gtattgttat caagcctatc agtaaccctt
    14881 ttgcaaagaa tggtctgtct tcatattcta taagacctag tcctactgga attgaaaata
    14941 ctaatgtaca tccaagcatg gatgacacaa ccaatcctgc aagcattcct gcctctttat
    15001 tgtgtgcaag ttccatagcc aatggatacc ctcccatatc attcgctaaa atagttgcaa
    15061 acatagatgg gtcagctcca cagagattga agattggtat tattatagga cctagtacat
    15121 ctgaaattac tggagctaaa caaactatac caaccattcc taaagccaat ggacccattg
    15181 aattaaaccc ttcttcaaac tgctcgccaa gtccaaattt atttccaatt attctatcta
    15241 acgctcctaa aagcactccc acagccatta tgtataatat aattttatca aagggcattt
    15301 ttaattcctc ctatacatgt aaaatactct attgtaactt aattagcaca cacctttttg
    15361 gtatcgtttt cccaattcat agctcttttt actgcttggt tccatccaag taatatttct
    15421 tctctttttt gagtagtcat atttggaata tattttttgc ttattgtaaa ttcacttcta
    15481 atttcatcaa tactgttcca gacaccacaa gaaagacctg ccatatatcc tactccaagt
    15541 actgtcgtct caggattttc tgcaatttca attggtatac ctaatatgtc ggattgaaat
    15601 tgcattgtaa agttattttt agtaattcca ccatctactc tcaacatttt tatttttttc
    15661 cctgaggcta agttcataga atcaacaaca tctttgactt gaagtgcaac agactcaaga
    15721 gtcgctctta ctatatgctc ttttttagta cttcctgtta tcccaaccat tgtacctcta
    15781 gcatattggt cccaataagg tgctgctata cctgcaaatg ctggtacaaa atatacacct
    15841 ccagtacttg atatggaatt tgccatgtca tcagtttctg aataattttt tataactcca
    15901 atgccatctc taagccattg tattgcagca cctgctatat acactccacc atcataagca
    15961 tatgtaactt tatctcctat tttccaagca acagttgtca aaataccatt acctgctata
    16021 tttagttcct cacctatatt catatatagg aaacatcctg taccataagt atttttaacc
    16081 attcctggtt caaaacatcc attgccaaat aaagctgctg gttggtctac cattgaagca
    16141 gtaattttta tagaaacccc acaaaatgcc tccttatctg tagttccaaa atcaccactc
    16201 gtaggcatta tatctgctaa aatacttcta ggtactccta taatgtcaag caactctcta
    16261 tcccaatcca tatctcttat attaaaaagc attgttcttg ctgctgtaga tacatcagtt
    16321 aaaaaagatt ttcctcctgt cattttccat attaaccaac tatctaatgt tcctgcaagt
    16381 acttctccat ttttaatttt ttcttttaca ccttttacat tatctattac ccatttaatt
    16441 tttgtacctg agaaatatgg gtctataatt aacccagtct tatctattat cttattttct
    16501 aaacctggta tattctttaa attttccaca taatcataag ttctcctgca ctgccacaca
    16561 atagcattgt aaagtggttt acctgtattt ttatcccata acattactgt ttcaccttga
    16621 ttatctatac caattccttt tatatcactt tcattgattc cattttcaaa agcctgttgt
    16681 aatacattat tactcacagt ttttgtatta ttccaaattt ctatagggtc atgttcaaca
    16741 tatccagatt gtggatatat ttgagtatgt tccatataat cgctaaaaac aaactctgat
    16801 ttttcgttta tcaatactgc tcttgttcct gttgttcctt ggtcaattgc taatacatat
    16861 ttcactttaa atgtcctcct ttattttcag aattaataaa ttctttttta ttttttgaat
    16921 atataatcta ttcaagttat ttatatttta tatcatcatg tggatttatg caatattcat
    16981 tacaatattt tttgttattt ttaattatag tgat
//
LOCUS       DAYAOX010000079        16886 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.079, whole genome shotgun sequence.
ACCESSION   DAYAOX010000079 DAYAOX010000000
VERSION     DAYAOX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.079"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            164..1174
                     /locus_tag="ACGI57_001948"
     CDS             164..1174
                     /locus_tag="ACGI57_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFG9717026.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            1310..1798
                     /locus_tag="ACGI57_001949"
     CDS             1310..1798
                     /locus_tag="ACGI57_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFG9717027.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1974..3902
                     /locus_tag="ACGI57_001950"
     CDS             1974..3902
                     /locus_tag="ACGI57_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9717028.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            4079..4489
                     /locus_tag="ACGI57_001951"
     CDS             4079..4489
                     /locus_tag="ACGI57_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFG9717029.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            4511..4813
                     /locus_tag="ACGI57_001952"
     CDS             4511..4813
                     /locus_tag="ACGI57_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HFG9717030.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(5030..5722)
                     /locus_tag="ACGI57_001953"
     CDS             complement(5030..5722)
                     /locus_tag="ACGI57_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFG9717031.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(5748..6935)
                     /locus_tag="ACGI57_001954"
     CDS             complement(5748..6935)
                     /locus_tag="ACGI57_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9717032.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            7271..9148
                     /locus_tag="ACGI57_001955"
     CDS             7271..9148
                     /locus_tag="ACGI57_001955"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9717033.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMTDSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            9505..10911
                     /locus_tag="ACGI57_001956"
     CDS             9505..10911
                     /locus_tag="ACGI57_001956"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9717034.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            10939..11787
                     /locus_tag="ACGI57_001957"
     CDS             10939..11787
                     /locus_tag="ACGI57_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9717035.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            12448..13140
                     /locus_tag="ACGI57_001958"
     CDS             12448..13140
                     /locus_tag="ACGI57_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9717036.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            13137..14072
                     /locus_tag="ACGI57_001959"
     CDS             13137..14072
                     /locus_tag="ACGI57_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9717037.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            14220..15143
                     /locus_tag="ACGI57_001960"
     CDS             14220..15143
                     /locus_tag="ACGI57_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717038.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            15127..15897
                     /locus_tag="ACGI57_001961"
     CDS             15127..15897
                     /locus_tag="ACGI57_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717039.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            15907..16689
                     /locus_tag="ACGI57_001962"
     CDS             15907..16689
                     /locus_tag="ACGI57_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717040.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
BASE COUNT         6152 a         2026 c         3078 g         5630 t
ORIGIN      
        1 ttgggtattg tatactggaa ttagagtaat taatttattt tttaacatac aaatgatttt
       61 atgaaaaaaa acaagactca aaaattttta tattctttga tagaataaaa ttgagagtaa
      121 atttattgaa ttttataaaa tttataaggg gtgagtctaa gatatgcatg aagataaaaa
      181 tgatatattt gaatcaatag atactgagga ttttgataat aaaacagaag aagaatgttt
      241 agagctagta agaaaaaatg gattgaattt aatatacata aaaaatcaaa cagaagagat
      301 atgtctagag gctgttagac aaaattgtaa tgcaataaaa tgtgttcaga atcagacaga
      361 aaaaatatgt atagaagcag ttaaacaaga ttggagaatg ttagaatttg tggaagagcc
      421 aacagaagaa atttgcatgt tggctattaa ccaagatgga acagcattaa aatatataga
      481 aaatcagaca gaggagttat gcttaagagc cgttgaaaaa aatggtgcag ctttggagta
      541 tgtaaaagaa caaacagaag aaatatgtat agaagcagtt agaaacagcg aatttggatt
      601 ggcaagagtg aaaaatcaaa cagaaaaaat atgtatggaa gcagttaagc attgtagcta
      661 taatctaaaa tgggtagaaa atcaaacaga agaaatatgt atggaagcag tgagacaaaa
      721 tgggcttgat ttgaaatttg taaaaaatca aacagaaaca atctgtttaa gagcagtaag
      781 acaaaatgga atggcattag aatttgtaaa ggaacagact gttggtatat gcttaaaggc
      841 agtaagacaa tatggaatgg cattaaaatt tgtaaaggaa caaacagaag aaatttgtac
      901 agaagctata aaacaagata aaagagcact tagttttgta aaaggagata aggaaaaata
      961 taaggctcta tatgataata atgaaccatt tgcaaagaga tatgttagaa atgtaataga
     1021 aaaagaaaac agagcattga ttaaaaaagg agaagaaaat gcaaaaatca aaattgcagg
     1081 aaaactttat gatagaggca tgtcttttga agatatatca gatatagttg agattgaaat
     1141 aagcaagtta aaagtgagtt taggtgtttt ttaaagtaat aaatgtaggt gttttttatg
     1201 ggaaatagtt aataaaaaaa taatcgacta gctctttgta ggacagcaaa attagtgata
     1261 taatttgatg tataataaat tatttttatt agataagggg gaattaaagt tgaagattac
     1321 agtattatac caaacaagaa gtggaaacac agaaagggtc gctaagctga tagaagaggg
     1381 cgctaaaaag gtggatggag tagatgtaaa gcttatgaga cttgataata ttgatttaga
     1441 ttatttaaat gaaagtaaag cagtaatatt tggaacacct acttatttag caaatacaac
     1501 ttgggaagta aaaaaatggt ttgatgaaga ttcaaaaaaa gttaatttag ctggaaaatt
     1561 aggagctgtt tttgctacat gtgattatat atgtggaggt ccagatgttg cgatacttac
     1621 tatagtagga catttgatgg ttaaagggat gcttgtgtat tctggaggtg gttctttagg
     1681 aaagccattt atacatttag gacatgtaca ttcaattgaa ggtccagagt tacaagatga
     1741 gaaggctata atatttggtg aaagaattgc tactaaggca aaagaactat ttgcttaatt
     1801 tctatatgca tagtaatgat tagagtaaat ttagggtttt aatggagatt tgaagtaaaa
     1861 gttataaaat aaaaaatttt ccataaaatt actcaagatg aattataata taattataaa
     1921 taaattatat tattaaaata tttaaaatca aacgtcaata gcgagggaag tttatggtat
     1981 ggcatataga atatgagatt ttttctgcaa ttatagttat ttttttaatg gtatactttt
     2041 ttagaagtaa atttatacct actttacaaa acaaaatata ttgtgcactt ttaatattct
     2101 catttttatt tataatttca aatattttag gttcattttg tctaaataat atagacaaaa
     2161 ttccaatttt tattactttt ttattgaatc agatatactt attgttattg ccaataccag
     2221 cagcacttgt atcattttat gttatggcta ttatttatca agatataagg tatatgaaaa
     2281 aaagattatt atttttatct ataccactta taatttgtat aatcttatct attacaaatc
     2341 cttttacgca tattctattt ggattgtcaa aagaaacagg ttatatacga ggttgtggat
     2401 atgttgcaac ttttgcatct tttttctttt atgcaatata ttcagcattt ttatctttta
     2461 aatatagaaa agcaatgcat gaatcaaaaa tatgggctat tagaggtttt cttatagttt
     2521 caattgtggc aattatctta caggcaattt ttttacagta tcttcttaca ggaactgcat
     2581 gtgcatgtag tttacttttg acatatcttt ctatgcagaa tcgaggtctt attattgatg
     2641 accttactgg tgtactcaat agacaatctt ttattcaaga gctagatttg aatatcaata
     2701 ctgaacaatc aggatttatt attacaattg cgcttgatga ttttaagttt atgaatgaaa
     2761 cttttggaac aaaaaatgga gatattgcac taaaagaagt tggaaaatat ttgattcaga
     2821 tacatgatgt aaaccatgta tatagaatag ggggagatat attttctatt gttgtcaata
     2881 gtcaattagg tatagaacca gatgatatta tcagaaagat agaagaaaga tttaaaaagc
     2941 cttggctagt agaagagata agttttaatt tagcaactag tattgctgtt gtttattacc
     3001 cagaaaatgc agaaacgaca gaagatgtaa ttgttgctat agatttctct atacatgaag
     3061 caaaacgctc tgagtcaagg cgagttgttt atgcagatgc gtctataagt gaaaagataa
     3121 agaagaaaca tgttattaaa aattgtttaa aagaagcatt aaaaaatgat gggtttgaag
     3181 tgtactacca acctatattt tctaatagca ggggcaggtt tacttcagca gaggcattac
     3241 ttaggctaga acacaaagag ttaggtttta tacctcctga tgaatttatt ccaattgcag
     3301 aacaaacagg attgattaac tcaattggat tagtagtctt tgagaaggta tgtagattta
     3361 tagctagtga tgagtttgaa gatttaaaat tggataatat agctgtgaat ctttctgtag
     3421 tacaatgtat gcaaaaaaac ttagctgatg atttacttta tcttatgaat aaatataatg
     3481 tatcaccaac taaatttaaa cttgaaatta ctgagactgt ggctatgggc tcttttaata
     3541 taattaaaga aacaatggaa agattaattg atttaggtgt taagtttgca cttgatgatt
     3601 ttggaattgc ttattctgga gttactaata tgctttcttt accattttca gtaattaagt
     3661 tagataagag tttgatttgg tctatgaatg aagatagtag gcataaactt acagttgaga
     3721 cgattatagc acttataaat aaattgaata tgaaggctgt agcagaaggt gttgaaacca
     3781 ttaagtgtgc tgaagattta atagctatgg agtgtgaata tttgcaaggg tattattttt
     3841 ctaaaccagt tccagaagat gtttttaaaa atttgttgaa agaaaatact tttacaagtt
     3901 aacacccttt atttttttaa ttacaacttt gttcattgac actgccagcg atatattata
     3961 aaattaacac aataataaat attaaattta ttatacatat tattaatatt aattaatgga
     4021 gtaaagattt attatatatt ttatttaatt atcctaaagt gagaaaggaa gatgtgacat
     4081 gaataaaaag aaaatattag tgccaattga tgggacagaa agaagtatgc attctttgga
     4141 atttataaaa ggaatattta aaaaagatga agttgaagta gaaataatga atgtaaaaga
     4201 actagtattt attgatggca tatccctggc tgaagagata aaaaattctg aaaatttagg
     4261 aagaagaatc ttggataaag cagcagagat aatgggagag tatgatgtta aagtacattt
     4321 tacttttgga tatccaggag atgagataat tagaaaagca aaagaagata atgtagattt
     4381 tatagttatg acaaaatcta ctaaaaaagg tcttacaaga atgataggtt cagtaacagc
     4441 aagtgtagtt aaacaagcaa aatgtatagt gatgattgta ccggagtaga attagaaagg
     4501 tatttgaaat atgaataaaa agatagtagc agtttgtgaa agtgaaagaa gtttaaattc
     4561 tcttaagaca gctgcaaatg atttaggatt tgatttagtt tatgaaattc aagaaaatga
     4621 taatataaaa aatgaacttt ctatttatga tatagaagat gcatccatag tgttatttgt
     4681 tacaagtgat tctatagaaa gcatagagaa gattgataga tttatagata gagaatttta
     4741 tgaagttgat ccaaagtata taatagagga tgcaaaatct atagtaagtg aaattttaat
     4801 agacttaaat taatcgtggg tgtcgcacta agaatagagc gacgcctttt tatatatttt
     4861 acacaagaaa taaagaggtt gtatcaaaat atagataaga gtctttttca ttgttgaaat
     4921 gatgaaaata tatttgattt catacaaaaa agagtacctc ataactaaaa agttcacttt
     4981 gagacactcc atttttatat gttagttgca tttaaggtaa aatagaagtt taatcatgtt
     5041 gaaataactg aattacatct tctaaatgat tgtacattga tgtataacct aagtaagagt
     5101 ctttactatc aaatgcaatc attatcttgt tatggtggct aatcatatcc ttgagagaat
     5161 ttctatattt aaaaccaaag tattggttag aagaaactgt tttaggtatt aactcataat
     5221 attttataaa aattggatta tgagaagcta aaattatctc tttgtgaaat tgcaagtcag
     5281 catcataaca ttcttctatc aaaccatgag aaaccttatc caattcctct attgacttta
     5341 ggtaagtata ttttattttt tgaagttctt tagatgtagc atttaaaatt gctagtttag
     5401 tagcttcgct ttcgataagt tgtcttgctc ttaaagattc tacataaaat tcttgtatgt
     5461 tatgattttt atagatttca ccatcatcaa ataggtcaac ttttttaaat ccttcaacta
     5521 taggacttga ataaggcaca gctctaagta ttccttcctt acagagtata tttatggcat
     5581 ttcttacagg tattctactt atgttaaata ggtcagcaat ctttctctca cttggtaatt
     5641 tactattaat tttaaaagtt ccatttagaa tgtttaattt aatataatca ataatttttt
     5701 cagtggcatt taagcttttc ataaaaactc ctaaacatat aattttttta tgattgtatt
     5761 atatcatagt taagagttaa atctactttt ttataatatg tataagacag gatagaggcc
     5821 aaaatagcta taatagccaa gataaagtat ccatattgta ttgaagtata ttccgatatt
     5881 ttaccaacta tcattggaaa tacagacatt cctatagcat aaattgcttg aaaaaaaccc
     5941 attgcagtag atttctgatg ttttggtaca tttttcatag actcgctagt aaggtaagaa
     6001 aggagtatac ctgtagatat acctggtagt acttgtagta caaatattat attaatattt
     6061 tttattgttg gaactaaaaa gcaataggct gaaactaaaa taaaaactaa aggaatccaa
     6121 aattttggtc catacttagt acaaaattta gtagaagcaa agccagaaga agcaacagca
     6181 cacaccatgt aaaatataga agatagacct atgagggtag aactagaacc taagttttta
     6241 agaatttgag tagtaaatga cattgttgta gacatttgaa taccctgttg aattaatgcc
     6301 ataaaggaaa aaaatacaag tcttttatcc ttgcagatag ataaatattt tttaattgat
     6361 attttttcag gtattttttt agaattatga ttttttatat ttaaggctag aatcgttcca
     6421 ataactgaag acaatacact catgatacat aaggctgtca ttcctaattt ttcataaaat
     6481 aaagtactca taacaaatcc aatcaacatt cctagattgt ttaccaatat tatcgtactt
     6541 gtagcttttt gttgatgttc tgtagaaaaa aagttggtat agtgaaccat gtaagaaatc
     6601 caagttgatg atgatattcc agataaaatg ttagctataa gaaaaccatt gcctccagga
     6661 gaaaagactc ttataagaga tgctaaacca gcaaagaaaa ttccagacag tataaatatt
     6721 ttatgtttat ttatggaatc agcaaatact ccaacaggaa gccttaataa acattgtgat
     6781 attccatatg cacctattat tataccaatc atatttgtag atacattaca tgcacttaaa
     6841 tatggatttt ggaaaggtat atatacatat tgtgaaaacc agaaaaatgt aacaatagaa
     6901 agtaaaagta atttttgatt agatattttt ttcatattat cctccatgtg gtatttttat
     6961 aatgcaaata atataccatt tagatttttt aatcaagtac tttctctatt aaaaaaaatt
     7021 taaatatata attattattt acaaaaaaat tatactttat agttgaatta agaaaacgct
     7081 tatgatacta taaagttaac aaatttgaat atataacagt agacgtgtta ctggtaatgc
     7141 aggcaagatc gaatcaattt ttatagatta tctatagagt actatgggta taatctattt
     7201 atttggttcg atttttttgt ttaattggcc ataaaacaaa aagcctatag ttgaaaatgg
     7261 aggatttttt atggattata aaaatatagc tcaagaaatt ctactaaatg taggaggaaa
     7321 agagaatgta aatgaagtga ctcattgtat gacaagactt agatttaaag tcaaaagcgc
     7381 ttctaaagtg aataaggata aattatctaa aactgaagga gtaataactg ttgttgagag
     7441 tatgggtcaa atacaagttg taattggaaa taaagtaaaa aaagtatatg atgaggtcat
     7501 aaaaattgta cctcaatcaa ataacatagg taaaaagaat gaaagtcaag aaaagcaagg
     7561 tataattaat tctatattat cagcagtagc tggtatattt acacctacaa tacctgctat
     7621 agcaggggtt ggtatgatta aaggtatttt atcagttctt gctatgtatt atatgaataa
     7681 aaatggagta gatataaaag aaacgcaaag ctatattatc ttaaatgcta tgactgattc
     7741 tatattctat tttatgccta ttatactagg atatactgcg gctaaagtat ttaatgcaaa
     7801 taaaataata tctatggtat taggagctac actttgttat ccaactttta ctgctttgat
     7861 ggcaggtgaa gaatctgtta gatttttagg attggcagta acaaaagcta cgtatacatc
     7921 atctgttatt ccaataatta ttgctatatg ggctctttca tacgttgaaa aagtattaga
     7981 aaagtatata cctgaggtaa ttaaaataat aatggttcca actttatcat tggtagtaat
     8041 gttacctgca acattatttt tatttggacc aatcggaatt tatataggaa atgtcataaa
     8101 cttttcttat aagtatattt atgaattaag cccagcattg tgtggagcat ttgtaggtgg
     8161 gttatggtgt gtgttagtta tatttggtgc tcatagagca ttattaccaa taggtataag
     8221 tgatgttgct caaacaggaa gacaaaattt attggcgttt gcaggagctg caaacttttc
     8281 acaagcaggg gctgctctag gagtattctt taaaactaaa aatcaaggac taaaaacaat
     8341 ttctatgtct gcaactataa cagctttatt tggtataact gaacctgcaa tatacggtgc
     8401 caatcttaga ttaaaaaaac ctatggtatg tgccgtaata tgtggtgcat ttggaggtgc
     8461 gataatggga atgggtggag cttatggtaa tgcatttgca aaccaaggtg ttttaactat
     8521 acctgtatat gctgaagcag gtgcattagg tttcttaagt tatctaggtg gttgtgctat
     8581 agcatttttt ggttcagcta tttcaactta tctagttgga tttgaagatt tggaagagtc
     8641 ttctaataag gaaaattcat ctataaaagt agaaacgaag gatggtgtta ttgatataac
     8701 ttcacctgta gagggcgaat gtatagaatt gtcagaagta aaggatgatg tatttgcttc
     8761 aaaagctatg ggagaaggta tagctgtgtt acctacaaaa ggagtaataa cagcccctac
     8821 tgattgtgaa gtagcttctt tatttccaac attacatgca ataggattaa aattagataa
     8881 tggagcagaa atgttaatac atgttggaat caatacagta gagttaaatg gtaaatactt
     8941 tacaaaacat gttaatcaag gagatttagt gaaaaaaggt gataaactta tatcgtttga
     9001 tatagataaa ataaaaaagg ctggttatga tgtaactacg ccagttatag taaacaatac
     9061 atttgatttt gggcaagtag tttcttgtaa atctagttat gtttcaacca atgataatat
     9121 aatttcatta gtattagctg gtaattaagt taatttgact agagataagt aaattgattt
     9181 aaaaggaaat aagtaaatca acaattaaat acatctaaaa gtaagtttat tgcaatgaat
     9241 tgttaaagaa aataattaat atggagtaaa tctattgcaa tgaagtaaca aagaaaataa
     9301 ttaatataga gtaagtttat tgtaatgaat tgttaaagaa aagagttaat atagagtaag
     9361 tttattgtaa tgaattgtta aagaaaataa ttaatatagg gtaaatctat ctcaatgaaa
     9421 taacaaagaa aagagttaat atagagtgaa cttattgcaa tgaatcaaca aagaaaataa
     9481 tcaatataaa aaggagaaat atttatgaat acaggatttc caaaagactt tttatgggga
     9541 gcatcatctt ctgcatttca agtggaagga gcttgggata aagacaataa aggaaaaacc
     9601 gtagccgact ataattcttt taaaaagtct catttacagg cagatactaa agtcgcatca
     9661 gacttttacc ataattatga agaagatatt gaattaatga aagaattagg gatgaaaaca
     9721 tatcgttttt caatttcatg ggcaagaata ataccagatg gtgaaggaga gataaaccag
     9781 aaaggtttag aattttataa taagataata gacaaactga tagagtgtga tattgagcca
     9841 ttcgtaactt tataccattt tgaccttcca tttaaattag tagagaaata taatggttgg
     9901 gaaagtagag aaactgtcta tgcttttgaa agatttgcta aaatatgctt taagcatttt
     9961 ggagataggg taaagtattg gcaacctcat aatgaacaga atttaattgt tagagtggag
    10021 gagagaatta atatatatga tgaaacagat tcatggaaga tagataaaat gcgtgctcag
    10081 atggattata atctttgctt agcacatgca ttagcagtca atgcatgtca tgagatgata
    10141 aaagaaagta aaattggagc agcagtatca tcatctgtaa catatcctct tacttctaag
    10201 ccagaagatg tatatgcagc tagaatgaat gataatttta aagtgtacta tatgcttgat
    10261 atgcatcatt atggagaata tccagggtat tatatgaagt atcttgaaaa aagaaatata
    10321 gttcctcaca tggaagatgg ggataaagag atacttaaaa aagctaaaat ggattttata
    10381 gctgtaaatt attataggac aaattgtgca gaagctttac ctgaagattc acaacatcca
    10441 tttggattaa gagaaggaac tgttgatttt agtatgtatg gactatttaa aatgtcaatg
    10501 aatcctaact tagaggcttc tgagtatgga gcagcaatag acccatcagg tcttagagtt
    10561 gccttaaatg aatattggca aagatatcac ttacctgtta taataacaga aaatggtcta
    10621 ggagctaaag atatattaga agatggaaaa attcatgatg actatcgtat agactattta
    10681 agaagtcata taaatgcatg taaactagct attgaagatg gtgtagagat gattggatat
    10741 tgtccatggt catttacaga ccttttaagt tcttctcaag gttttaataa gcgttatgga
    10801 cttgtttata taaatagaac tgaccatgaa gttttagatt taaaaagaat caagaaagat
    10861 agtttttact ggtataaaga agttatagag aacaatggaa tagtaaaata aacataaata
    10921 tatattgagg gttttagtat gtacattaca aaggtattaa ataattcgtt gctacttgca
    10981 aaggatgata atgatgaaga aataatattg atggggaaag gcattggcca taattataag
    11041 gccgggtctg agttaaataa aaaagatata gaaaagattt ttgttcttca tgatgaaaat
    11101 ataaaaaaga gtattataca attggcaaca gaaatagatg cagaatattt tggtatagct
    11161 caaatgataa ttgcatatgg aatagaaaaa tataacttaa aacttatgaa ccatatatat
    11221 ttggccttaa ctgaccacat tgcctttgca gtgagacgtt ttaaagaagg tataatgatt
    11281 gaaaatcact atttgttcga aatcaaggaa tttaatccaa aagagtatga tataggaaaa
    11341 tatgctatta ctgtttttaa ggaggttctt ggtttagacc taccagaaga agagattggt
    11401 aatatagcag gccattttat aaatgcacaa caagataatc cttatagtga tagaaataag
    11461 agaagtgcta aaattgtaaa tgctattttg caaatagttc attatcactt ttctattgta
    11521 tataataaag agtcatttta ttataggaga tttgtaatgc atctaaaggc cttttcacaa
    11581 agatttctgg ctaatgaacc atcaagagaa aaaatagatt ttatatatga gcaagttcaa
    11641 aaaaattgta aggaagagta tgaatgtgtg aaaaaggtag gagcttatat gtataaggaa
    11701 tactccagag aattaccaag acaagaggag ttatatttga tgattcatat tcataagatt
    11761 ttaggagaat tatgcgaaaa tagttagaat atcttaatat gctagtaata ttataaaaaa
    11821 ttaaaaatta actgcacttt aaatatgaat aattgcatta cttaaggggg ttgtcttaaa
    11881 attgattttt ttaattaaat ttgaagctca actccttttt taaaccttta tatcattaca
    11941 actttaatgt ttatacgact gtactttaga tgtatatcta ctttttacat ttctcaacat
    12001 gcaaacctac cattttttgc agtatgttaa ttgaaataaa ccattgagtt aaccctaaat
    12061 ttattctgga cttataggaa tagttccaaa gatactttaa aatattttct ggaataattg
    12121 cttatttcac atatgtttta ttgtgatgtt ttgatttaat aaattcttca actctgtata
    12181 tagtaaatgt attatcatgt ttgttttatt caagcacact tataatacgc ttgatttttt
    12241 cacaatgatg aaatatacta atgggtgtta taggtataag aatcgcatta cacttgaaga
    12301 agtaagtgat aaatgaggta taaatcctca tatgataaac tactatttca ttgaggaaca
    12361 tattctaagt gatgtcaaga tactacaata tgactaaatt cctagtgata aaaaagtcta
    12421 cgaatgagtg tagaaagggg aactaagatg aataaagttt taattataga tgatgataaa
    12481 gatctttgta ttttgttaaa aaagagtatt tcaattgaac agattgaagc aaactattgc
    12541 cattctggga gagatggact ggttgaactt tcaaaacaag attatcagtt agtagttcta
    12601 gatgtgatga tgccaggtat ggatggtttt gaaactatgg aacaaatacg aaaatatagc
    12661 agtattccca tcttaatgct gacatcaaaa agcgataatt acagcaaagt gcatggattg
    12721 cgagctggtg cagatgacta tctaaccaaa ccatttgaaa ttacagaatt tactgctcgt
    12781 gtactgtctt taatccgacg ttacacccgt tttaatccaa aaaatgagga gccacagcaa
    12841 ctttctttca aaggtttgtt gattgacctt gaccagcata cagttactac caatggcgaa
    12901 cctatgttgc ttccacccaa ggaatttggt gtacttcttt actgtgcaag gcatcagggt
    12961 aagattttga caaaacagca gatttatgaa gccgtgtggg gtgagccata tgtatatgat
    13021 gacagcaata tcatggctat tatcagtcgc ctaagaaaaa aaattgaact agacccatca
    13081 tctcccatct atttgcaaac aataaaagga attgggtatc aattcaacac ggaggtatga
    13141 aaatggatga acaaacgata attcttttct tactcttgct gtcgattggt ctactttttc
    13201 tttccatatt tgctttttcc aagcttgggc atatttacaa gcgcctaaaa gatatagagg
    13261 agatactggc tgatgtacaa attggagacg aaaatcgtaa aatactaatt aagccatgtg
    13321 atgttatggc tccattggtt tatcagctta atgagattgt ctacgattat gaaaataagt
    13381 tactttctct gaaaaagtcg gataaagcta gcaaacaact tatgacaagt ttatctcatg
    13441 atgttcgtac accattaaca actctgattg gttatctgga tgctgtacac agtggtatag
    13501 ttatcgaaca agagcgggag gaataccttg aaatcgctag gcgtagagcc tacgacctca
    13561 aggattacat tgatgttctt tttgactggt ttcggttgaa ctctgatgaa tttacgctgt
    13621 caatagaatc tgtggaaatt gcagagttgt cacgcaatat tttgaaagac tggattccta
    13681 tattccatga aaagaaactg aattttgaaa ttgatattcc agaaaatcga ctaatggtaa
    13741 acctcgaccc agatggatat tcccgtgtgg tcaataatct ggttcaaaat gttcttgctc
    13801 acagtaaggc tcggcaaatc aagattacca tgagtgagga ttccagaatg gtattactcc
    13861 gtgttgagga tgatggtgtt gggattgcca gagaaaattt accacatatt tttgagcgac
    13921 tgtataagtg tgataaagga cgttcagaaa aaggaagtgg tcttggtcta tctattgtca
    13981 gtcaaatggt ggagcgaatg ggtggtcagg tttccgtaga aagtgagatt ggaaagtata
    14041 ccgtatttac agtgagctta ccattaatat aaaattgttt ttcccccatg tgacatgtat
    14101 acacatgggg attttttgta agttataaag ttgcaaggtt aatacaaggt tgttgcaagg
    14161 ttaatgtaag attgaggtgg tatgataact ttaataaaca aaggaggctt ttattattta
    14221 tgaataattt aattattgaa acaaaaaatc tcactaagca atatggtgct caaaagagcg
    14281 tttctaattt gaatattcac gttcagaaag gtcgcattta cggattgcta ggaagaaacg
    14341 gtgcgggtaa gacgacaact atgaaaatgt tattgggtct aacaaaacca actactggag
    14401 aaattaaaat atttggtcag gacattcaca ataacgagaa gaagcttctt cctcgaattg
    14461 gatgtttgat tgaatcacca gggttttatc ctaatctgac aggaacagaa aatctcaaaa
    14521 tctttgcaag gcttagagga ataccaaaaa aaaatgcagt agaatcagca ctggatgtag
    14581 tcggtttgcc aaacaatgac aaaaagttat tttctcaata ctctcttggt atgaaacaga
    14641 ggttggcgat tgctcttgcc attatgcatg acccacaact gttgattttg gacgagccta
    14701 tcaatggact tgatcctatt ggtattgcag aagtaagaac gtttattcgt gatctctgca
    14761 cagaacgagg taaaactatt ttgatttcca gtcacattct ttctgaaatt gccttgttgg
    14821 cagatgacat tggcattatt gaccatggta ttttgttgga ggaagaaagt cttgcccaat
    14881 tagaagaaaa aaacagtaaa tacatacatt ttgctgtatc tgatgcagca caggcagcaa
    14941 ggcttctgga aacccaactt aacctgaaaa atttcaaagt agcagataat tacaatatta
    15001 tgctatatga tacagatgtt tctgttgcag aaataaatcg tgctttcatt ctaaacaaca
    15061 ttgaggtatc agaatctcat ttatgcgaag atactcttga agattacttc aagaaagtaa
    15121 cagggggtga aggaattgct tgatataatt tcatgtgaat tttcaaaact caagcggtta
    15181 aaatttatac tgatttctat tttaggtgct tgcttatttc caattcccgc aacaattttg
    15241 attgcaaagg acaacttgcc ctttgagcaa ttgttcaaat tagttgtcaa ttttggatat
    15301 tttttgctat taccaattgt attaagtatt gttgctagcc aactattttt tatagaaaga
    15361 gataatgatg tattaaaaaa tcttgcaaca gtgccagtgc aaaaaggaaa actggctctg
    15421 gcaaaattgg ctgtgttgct gtttatatca ttgttctact ctgttgcagg gttaggagct
    15481 actatcattg gaggtttcat tgttggaata gtagaggggg ttgctgtaaa attaggaatg
    15541 agcattgctt ttggaattat gttttttgtt actgtacttc ctgttgtggt actcattgta
    15601 tatttcaatc gcagctatat tttttctatt atactttctt ttgtatatgc tattttcaat
    15661 tttagtatat ccttaaatat tatcaatttt gaaccaaata acccaatatt aagtgtatta
    15721 cctgctcctg ttataatgcg ttggtggatg gcattttggg gagatcccac tggcgaatac
    15781 acggcactta gacagccata tttgctctca actcctgctt gtgtaggtat tttatgtttg
    15841 ataaccatta tagcagtcct gctgatttgt atgatataca aaaagcagga gaattaggag
    15901 gtatctatgc ttgatataat tactacagaa tttcaaaaaa taaaaagata taatattcta
    15961 tggattggtg ttgttgctgt tctgttttca gcactattgg cagtttttca ggttgtaagt
    16021 tcacacggca gtgaccctct aacgtatgag agctttgcaa acggagttat ctggaataat
    16081 ttcagtctta gttttccatt tgggattaca cttattggag gatatcttat taaccgtgaa
    16141 tatacagacc aaaccttaaa aaacatccta actgttccta tttcattacg caaattgcta
    16201 gtgggaaaat taattgcggt tggaggaatt actgtattgt taagtctttt cagcttctta
    16261 tgcacattta ttttggggtt gttgttttgc aatatcaata tatctgttac actcgttgta
    16321 aaatccttag ttcagatttt gtctgttaat ctgtattgtt atgttgctgt tttgccgatt
    16381 attgcttatt ttggtcgtaa acaaaatgca tttctgacgg gtgtagggat tgcatttgta
    16441 tacggttttt gtggtgtgtt tgttgctgga agaaatctga cagactttta tccaatcaca
    16501 gcaggtcttg gtctagttgg atacaacaat ggtgctggta ctgtttatca gccacttatc
    16561 ggtgctatga ctttgattgt aattctaatc ttgacaacta ttctacttgt atttacacct
    16621 aattatgata aagttatgac catatctaag aaaaaaggga taaagcataa aaagtattct
    16681 cataaataac ataaatattg tttctattat ttaagttgct agacagtgaa aagaaaaaat
    16741 ctgtcgtaca gtgtctatat gctcatgtat tcaagaagct acagttttga tttttcataa
    16801 ctgtagcttc tttttgtatt tattaaatca cgactatcat aatacttgca ataggtatag
    16861 cgacaagaga gagtactgtc atatgt
//
LOCUS       DAYAOX010000080        16705 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.080, whole genome shotgun sequence.
ACCESSION   DAYAOX010000080 DAYAOX010000000
VERSION     DAYAOX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.080"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            331..852
                     /locus_tag="ACGI57_001963"
     CDS             331..852
                     /locus_tag="ACGI57_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9717041.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            886..1662
                     /locus_tag="ACGI57_001964"
     CDS             886..1662
                     /locus_tag="ACGI57_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFG9717042.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDKI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            2004..2957
                     /gene="gpr"
                     /locus_tag="ACGI57_001965"
     CDS             2004..2957
                     /gene="gpr"
                     /locus_tag="ACGI57_001965"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HFG9717043.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2975..3994
                     /gene="spoIIP"
                     /locus_tag="ACGI57_001966"
     CDS             2975..3994
                     /gene="spoIIP"
                     /locus_tag="ACGI57_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HFG9717044.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            4025..4276
                     /locus_tag="ACGI57_001967"
     CDS             4025..4276
                     /locus_tag="ACGI57_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717045.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4558..6363
                     /gene="lepA"
                     /locus_tag="ACGI57_001968"
     CDS             4558..6363
                     /gene="lepA"
                     /locus_tag="ACGI57_001968"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFG9717046.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6512..7849
                     /locus_tag="ACGI57_001969"
     CDS             6512..7849
                     /locus_tag="ACGI57_001969"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFG9717047.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            8082..9395
                     /locus_tag="ACGI57_001970"
     CDS             8082..9395
                     /locus_tag="ACGI57_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9717048.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9567..10742
                     /gene="hemW"
                     /locus_tag="ACGI57_001971"
     CDS             9567..10742
                     /gene="hemW"
                     /locus_tag="ACGI57_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFG9717049.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10845..11864
                     /gene="hrcA"
                     /locus_tag="ACGI57_001972"
     CDS             10845..11864
                     /gene="hrcA"
                     /locus_tag="ACGI57_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFG9717050.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11894..12514
                     /gene="grpE"
                     /locus_tag="ACGI57_001973"
     CDS             11894..12514
                     /gene="grpE"
                     /locus_tag="ACGI57_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFG9717051.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12606..14453
                     /gene="dnaK"
                     /locus_tag="ACGI57_001974"
     CDS             12606..14453
                     /gene="dnaK"
                     /locus_tag="ACGI57_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFG9717052.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14664..15818
                     /gene="dnaJ"
                     /locus_tag="ACGI57_001975"
     CDS             14664..15818
                     /gene="dnaJ"
                     /locus_tag="ACGI57_001975"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFG9717053.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
BASE COUNT         6747 a         1839 c         3038 g         5081 t
ORIGIN      
        1 aatacaaact attaacatac attttgatag tatttccaat aattataaga taatacaaga
       61 aattactcat ttttgtaaat ttaatgaaaa attataaaaa tataaattta tatgcttaat
      121 taagataatt tttttcatta gtatgtatga tagatgcatc aaacaatata actaatatgt
      181 tagaaggtta tatgtaatta atgtcagtgt ttatatagaa tagcatttgt agcatttatt
      241 atagttgtaa ttactattta tatcatagac ttgaatagtc taaaaaaata gactataata
      301 tttttataga ctaaagaaag ggctgataag atggacatag caaaactaat tttaaatcct
      361 gcaaggcttc gtattttaca atatattcga ctttatggca gtgttcgtac atctgatatt
      421 gtaaagtatt taaatgatat tcctcgtgca acagtttatc accatgtaaa aattttagaa
      481 gagaacaata tgattgaagt aattaaagaa aatcgtgtgc gtggtactat tgaaaaggtt
      541 tatgcactaa aggaatatac aacatctatg gaaggagaga ccttcgttgc actttctaca
      601 gcattttatg taggtctgat gcaggaaatg aatgaatact ttagcagaaa agagcaagac
      661 cataaaaagg ataatgtatt tttctcttca gcattactat atatgagtga taatgaatat
      721 gaaaatcttc ttaaaagtat agctgattta ttgaaaccat atatagaaca aaaacctaaa
      781 tcaaatctaa aacttagaaa actttcaatt atatcttctc cacctgtagc tgataatgat
      841 aaaacaaaat aacatgcaac ccatttttat aaaaggaggt tatatatgaa atttcatgag
      901 tttggaaata aaaacagacc acatattatg ttgattcatg ggggagggaa tgcatggtgg
      961 aactatttgc gacaagcaga agttttgtct aacaaatatc atgtgatact tcctacattg
     1021 gatggtcatg gagaagaata tgaaacaaca tacatttcta cagaggatac agcagataag
     1081 ttgatatcat atatagatga aaattgcagt ggacatcttt tcgctttgtg tggggtatca
     1141 cttggtggtc aaattgtcat ggaactttta tctcgaaaac ctaatattac taaaaaagca
     1201 attattgatg gtagtatttg ctacccaaga cctcttatgg caaggttttg tattgctggt
     1261 attcgatttt ttggaggact tttgtttagt gagaaggctt gtcgctttca aattgctatg
     1321 atgccaaaat tgttacctga aaatatgcaa tatccagata aaataaaagc gtattatatg
     1381 acagatatgc cacatgtaag aaaagaaaca ttctataata tgtatcgtac ttacatgatg
     1441 aactatacac ttaaagagaa tgttaaaaaa accacagcac aagtaatgta ctggtatgga
     1501 gaaaaagaaa tgaagtgtgt caaaaaatct gcaaaactgt ttcagtctta tgtgccctct
     1561 tgtgagattt atgaagctaa gagctataat catgggtatt tatcacttta tcttccaaat
     1621 gaatggttgg aaattgcaga accatttttc caaaaggaat aaaggtgtta ggtgattatt
     1681 gtataattat tgctgttttt ttatatagta aataaattag gtataaatat aaaaaattat
     1741 tttagttaat atattagtga aagcttaata ggaaaattat aaatagatta aaggtagcac
     1801 ctatataaat agttgctacc tttttctgtt tgtaaataaa tgatagataa gaaggtttac
     1861 tgacaaatca ctgactaaac ccaattttct tacattgaaa gccttaattt tacttgtttt
     1921 aaataaattt acaatttctt gttataaaat actaaatttt gttaacaatt ataattagca
     1981 tttaatttat actggaggtt tatatgatta gtgtaagaac agatttagct ttagaagcta
     2041 gtgaaatgtg tgaaaagtct caagaaggaa gtagtattcc tggtgtaaaa atagaaacta
     2101 aagaattaga aaattgcatt gtaactaaag ttgaagtaat agacaggcaa ggcgcagaga
     2161 taatgaataa agatatagga aagtatataa ctctggaaag taatcttatg aagtttgatg
     2221 atgatgagtc aagagaagaa atgataagtt atttaaaaga ggagttagta gatatatttg
     2281 gccaagacaa aaataaaaaa accctggtaa ttggtcttgg aaatagaaat attacttctg
     2341 atgcgttagg accaaagagt gtatcaaaaa cacttgtaac tagacatctt tttaaaaatt
     2401 ataataaaga ctatgatgat gatttcactg aggtatcagc tttaagccca ggagttatgg
     2461 gtgttactgg aatagaaact agtgaaatag taaaatcatt ggttgagaag gttaagccag
     2521 atagagtagt agcaatagat gcattggctt caagaaaaat ggaaagagta aactctacta
     2581 tacagatatc aacagcagga atatcacctg gtggaggtgt tggcaatact agaaaatctc
     2641 taactaaaga aactttaggc gtggatgtta ttgcaatagg cgttccaacg gtggttgatg
     2701 cagcaactct tacaatagat gtcttagata tggcaataga caatttaata gctcaatcag
     2761 aagaaacaga aagtttttat gaaatgttga aaaaacttaa agaagaagaa aaatatcatc
     2821 taataaaaga ttcgttagac ccatatgaca agaacttaat agtaacacca aaggatatag
     2881 atgatacaat cgaaaatcta tctataataa taagtgaagg cttaaatagg tctttacatc
     2941 caggtagatt ggtataaaat aaggaggagc aattatgttt aaaaaatgca ttaaagtagt
     3001 aaccttgact tttatattag tatgcatttt accaggaaaa tcattagctt tgaatcaaga
     3061 tgatttttta aagtttttag taaattcatc ttatccagaa gctaaagtcg aaggaaatga
     3121 tacagaaaat aaaaagaata ataaaaataa agaaacaagt aaggaaaata aggaagaaag
     3181 taaagaagga aacactaaat ctaaagatgc aagtaaagtt gataataaaa aagagagtga
     3241 aaaagaatat ataaaacttt atgttggtaa agaaaatgtt ccagatattg agtcaaaaaa
     3301 ttcagacaca actgaaacta acacaacatc tagttcagat tataaggatg atttaagagt
     3361 aaccaaagaa aatccaagaa ttttgatata tcatactcat ggatgtgaga cttattcaaa
     3421 ttcacctgat ggaaattatc attcaagaga taagaaaaat tcagtaatgg aagtaggaag
     3481 tgcattaact agtgctttag atagcaaagg atggggtgta gtccatacta ctaaatacca
     3541 tgactatcca tcttataata actcttatgc aagtagctta aaaacaatac aaagtatatt
     3601 acctaaatat aactcagtgg atatagcgat agacttacat agagatgcta gggatttaac
     3661 taatccagcc actaaggaaa aagaccattt aaaatataca actatgataa atggagagag
     3721 agtatcaaag ttcttctttg ttgtaggagg taaaaataca aatagaaaac aacttagagc
     3781 tttagcagaa gatataactg cttttgctga gaaaaagtat cctggtctag tatctccaat
     3841 agtagaaaaa gactatgcta gattcaatca gtttgcagtt aagaatcata tgttagtaga
     3901 aatagggaat aacgcaacaa gtgtagaaga atctaaggct actacaaagt atttagccga
     3961 gattttggat gaatatttta aacaaaaaaa ttagagcttt aagttataat taggagcatg
     4021 ctaaatgagc cgattagaaa aaaatataga aagtaaaagt aaactaagaa aatacaagta
     4081 tattatgaga atcttatttt tgattactat gattatatgt acatcagttt gtgtatttat
     4141 agtggacagt aatgcaaaaa gtatgctagg aatgaaacaa tatgattttc caagtattaa
     4201 atttgatata acaaaatttg attttgacat accaaatttt gatatacatg aaatcataaa
     4261 aaaatttaat aagtagtaat aagtgaataa ggtttattga ttaaaataag ccttatttta
     4321 gttatatatt tttaatgtat aaaataaaaa ttcattaact gtattttata ggatttactt
     4381 taagtttatg atattatata atatttagtg ttagacttga taatccacat ataatgtgga
     4441 taattttgtt tagtgccaat tagtttatat tcaaaaattt taaatttggt atataaagag
     4501 tttttagata atgtaaaata ataactataa gaataatcaa gaggagggaa ttttaaggtg
     4561 gacaataaac aaagtagaac tagaaatttt agtataattg cacatataga ccatggaaag
     4621 tctaccttag ctgatagatt aatacaacaa actgggcttg ttagtgaaag agacatgaaa
     4681 agtcaattac ttgacaatat ggaccttgaa agagaaagag gaatcactat aaagcttcaa
     4741 aacataagat taatgtataa agcgaaggat ggaaatgaat attatttaaa tcttatagat
     4801 acacctggac atgtagactt taactatgag gtatcaagaa gtttagctgc atgtgaagga
     4861 gcactattag tagttgatgc agctcaaggt gttgaggcac aaactttagc caatgtttat
     4921 cttgctatag accaagattt agaaatatta ccaataataa ataaaataga tttaccaagt
     4981 gcaagaccag aggaagtaaa aaatgaaata gaagatttaa tagggcttga ttcaagtgaa
     5041 gctcctctta tttcagctaa aactggttta aacatagaag atgttttaga agatattgta
     5101 aaaaatgttc ctccacctaa aggagataat gaagctccat taaaggcatt aatattcgat
     5161 tcttactatg atgcttacaa aggtgttgta gcttatgtta gagtatttga aggaactgtt
     5221 aaaaagggta tgacaataaa aatgatgaat accaataaaa agtttgaggt aactgaagtt
     5281 ggtgttatgg ctccaggtca aactgaactt agtgaattat cagcaggtga tgttggttat
     5341 attgctgcta gtataaaaga tataagaagt tgtcgtgttg gtgatacaat aacagattca
     5401 aataatccta cagaagaacc tttaccagga tacaaaaaag ctactccaat ggtatattgt
     5461 ggtatatatc caggagaagg agaaaagtat gagaatgtta gagatgctct tgaaaagctt
     5521 caagtaaatg atgctgcact tgaatatgaa gctgagactt cagcagcact aggatttggt
     5581 tttagatgtg gtttcttagg tcttttacat atggagataa tgcaagaaag acttgagaga
     5641 gaatttaacc ttgatataat tactacagct ccatctgtta tatatagagt tacaaaaatg
     5701 gatggagagg ttgtaatgat acaaaatcct gcaaacttac cagaaccatc tgagataaag
     5761 atgatagaag agcctatagt aaaaggagat ataatagttc ctaaagacta tgtgggtgtt
     5821 gttatggagc tttgccagga aagacgtgga aatatgttaa atatggaata tatagatgat
     5881 agaagagtaa tgcttcacta tgaccttcct ctcaatgaag ttgtttacga tttctttgat
     5941 gctttaaaat caagaactag aggttatggt tctcttgatt atgaggttaa aggttatgta
     6001 gcatctacac ttgtaaaact tgatatatta atcaataaag agcaagtgga tgcacttagt
     6061 tttattgtcc atgagacacg tgctttccct agaggaaagg caatgtgtga gaaattaaaa
     6121 ggagaaattc caagacatca gtttgctata ccaatacaag ctgctgttgg taataaggtt
     6181 attgcaagag aaacaataag cgctctaaga aaagatgttc ttgctaaatg ttatggtgga
     6241 gatatatctc gtaagaagaa acttcttgaa aaacaaaaag aaggtaagaa gcgtatgaga
     6301 caaattggtt ctgttgaagt tcctcaaaaa gcatttatgt ctgtgcttaa attagatgag
     6361 taaatcctta taaatttaga taaagatatt taaaagctag agtgatttta gaattatttc
     6421 taaattagtc tagctttttt atggtatatt ttaagtaaac tcatagagaa tataagtaag
     6481 aatattaaag aagaataaaa aggtgatatt tatgattaat atagaaattc ctaaaaaagt
     6541 agactatata attaaagaat tagaaaagaa tggttatgaa gcttatatag ttggaggatg
     6601 tgttagagat tgtttattag aaagagttcc aaacgattgg gatataacaa ctagtgctag
     6661 accagaagtg gtagtagaat tatttgaaaa aacaattcca actggtatac aacatgggac
     6721 tgtaacagtt atgattgaac atgaaccatt tgaagtgacg acttatagaa ttgatggaaa
     6781 ttatagtgat ggtagacatc cagattcaat agaatttaca aataacatag ttaaggactt
     6841 gtcaagaaga gattttacta tcaattcaat tgcatacaat tcaaagacag gattagtcga
     6901 cccatttaat ggctatgaag atatacaaaa taagtatata cgttgtgttg gaaatccagt
     6961 agatagattt gaagaagatg cacttagaat gcttagagca gttagatttt cagcacaact
     7021 caattttaaa atagctgaag gtactaagca aagtattcat aaaaaagcag atttgataaa
     7081 aaatgtttct attgaaagaa tccagacaga gtttaataaa atattagtat ctgattcatc
     7141 taaattaaac ttattaaaat caacaggttt gttgaaattt ataattcctg aaatatgtga
     7201 attagaagat gttactcaac acaatccata tcacatctat gatgtacaaa aacatacttt
     7261 aatagctaca gaagtaattg aagatgaact gtatttaaaa cttacaatgt tgtttcatga
     7321 tttaggtaaa aaagtaacta aaacaactga taaaaatggt gttgaccatt tttatactca
     7381 tagtagagaa tctgtaaaga ttgcaaagaa gattttgaaa agacttaagt atgataatta
     7441 tactattaat aaagttttaa tattaataca atatcatgat tatagaatag aacctaaaag
     7501 gaagataatt aaaaagttat taaataaact tgaagatgtt gaattatttg aagatttaat
     7561 aaaagttaat tgggctgata cacttgcaaa aaatccaaag tatgcaaaac aaaagatatt
     7621 aaatcttatt gagtgtgaaa aagaatttaa acatataata aatcaaaagg agtgctttaa
     7681 tcttaaagat ttagctatta atggaaaaga tttgatatct ataggtataa aaccaggtaa
     7741 agatatagga tatattttaa ataagatgct tgaaatagtc ataaataatc cagagttaaa
     7801 tgaaaaagaa atactaaaag aaaaagcatt aaatatatat acattttaga ttcaaatata
     7861 aaaaatttaa ttactttaaa aaacatagaa taaaatactt atttagataa aataaataat
     7921 ttttagagaa tattttagaa gttacgattg tatgattttt aaatgcatag gcatttactc
     7981 taggactata gactaatata aaaaaaaact ttgggaatac taaaaccata aatttataaa
     8041 attaatattt tatatactgt aaaacagtga ggagggacag tatgcatact caactacaaa
     8101 agactattgg tctttcagca gctctttcta ctgttgtagg tatggttata ggttcgggag
     8161 ttttttttaa gccacaagca atatacacta caactaatgg agctccaggt ctaggtatta
     8221 tagcgtggct tttaggtggt tttataacaa taactgctgg gcttacagct acagaaattt
     8281 cagctgctat accaaaaact ggtggtatga tgatttatat cgaagaaata tatggagaaa
     8341 agttaggttt tttaactgga tggatgcaaa ctgtattatt tttcccaggt acatcagcgg
     8401 cattaggagt aatatttgct caacaagcat ctgaactttt aggaatgagc ccaaataata
     8461 tggcaaatgt acttcctata gcaattggag ttatactatt tttagcgtta ttaaatataa
     8521 taggttcatc cttaggagga aaagttcaga cagtagccac tattggaaag atgatacctt
     8581 taattttaat aatagtcttt ggatttatta aaggtcaaag tagtgaagta ttaaatccat
     8641 ttgtaggaga tggtgtaaat gcatcaaatg cacttggaca agtattaata gctactttgt
     8701 ttgcatatga tggttggata aatgttggag ctatatcagg agaaatgaag tctccagaga
     8761 aagatttacc aagagcaata gttggtggtt tatcattagt tatggcagtt tatattataa
     8821 taaatatagc ttatctttgg gtagtaccag caagtgaatt agcaacagtt acttccccag
     8881 ctacattagt agcaacaaga ttgtttggaa atataggtgg aaaagtaata actgtgggta
     8941 tattaatatc agtatttggg acattgaatg gatatttatt aacaggttca aggatacctt
     9001 atacattagc tgaaatggga actttaccag catctaaaac tcttttaaaa gtaaattctg
     9061 gaggttctcc tgtaaattca atactactta taactgtgtt agcctgtgta tatgcattat
     9121 ctggtcaatt taatcttttg acagacttaa caatattttc aatatgggta ttttatgtat
     9181 tgacttttat aggtgttatg aggcttagaa gacaaaagcc agatttacac agaccatata
     9241 aagtcccttt atatccaata ataccaatta tagctatatt aggtggactg tttgtaataa
     9301 taaatcaaat attgacttca acagtcatat ctttaggcgg aatatttata acactattag
     9361 gtttaccagt ttattattat atgaaaaaaa gataactact taatttaagt tagataaatt
     9421 tgggtaaagg tgaaaattaa cttttatcca aattttttat actgttatac aatataagta
     9481 ttatatattg cttgatgtgc tattttgttt gatttataat ttagtaattg atgttttaag
     9541 ataatttaag aaatgaggag tgaaagatgc taggacttta tgtacatatt ccattttgtg
     9601 tgaaaaaatg taagtattgt gattttaatt cttataaaat ggacatagat tcaaaaaaaa
     9661 gatatataga agacttaaaa atagaaatgg aattatatag taataaatta tataaagata
     9721 ataaatataa aaataaagaa tgctgtagtc taaataaaaa tgacaaaata acgagtatat
     9781 ttgtaggggg aggtacacct agtatactaa cctctgatga aataagagaa gtatttataa
     9841 gtataaaaga aatgtttgat atagatgaga atgcagaaat aactatagag tgtaatcctg
     9901 ggacattaac attagaaaaa ttaaaaacta tgaaagagat tggcataaat agacttagta
     9961 tagggctaca agctattcaa gaaaagcatt tgaactttat aggaagaata catacttatg
    10021 aagaatttga gaaaaactat aaagatgcat taagtgtagg ttttaaaaat attaatatag
    10081 atttaatgta cagtcttcca aatcaaactc tatgtgattg gaaggaaact cttgaaaaag
    10141 ttgtacactt aaatccaaca catatatcag cttattccct tatactagaa gaaggaactg
    10201 aactatacaa tatgtatgaa agtaataaat ttgaacttat agatgaaaat gtagatatag
    10261 aaatgtatga gtatacaata aactatttaa agtcaaaagg atataatcaa tatgaaatat
    10321 caaactactc aaaagaaggc tataattgtg agcataatat tttatattgg gaatgtgaac
    10381 attatatagg gattggagca ggagcttctg gctatattaa tgagaacaga tacaataatg
    10441 tagaatcttt agaagattat catttaagtt tggtaaaaag agaaaaacca atacaagaaa
    10501 atgaaattct ttctgagaaa gatatgatag aagaaaagat atttatgggg cttagaatga
    10561 ataaaggcat aaaatttgaa gattttaaaa aaaaatttgg aatagatttt agagagaagt
    10621 ataataagca aatagaaatg ctattagcta gaaagcttat aaatcagagc tttgaaggta
    10681 tacaattaac tcaaaaagga agagaaatat ctaatagtgt atttatagaa tttatggaat
    10741 aatttttaag gtgttgacaa aataaattta taatggtata taatatatat aatcttttta
    10801 gcactcgaac ttagtgagtg ctaacagcta ggaggtataa taatatggaa ttaaatgaaa
    10861 gaaaattaaa tatcctcaaa gctatagtta aagattatat agaaacagct gaagctatag
    10921 gttctagaac aatatctaaa agacatgatt taggtgttag tgctgctacc ataagaaatg
    10981 aaatggctga ccttgaagaa ttagggtatt taattcagcc tcatacttct gcgggtagag
    11041 ttccatctga aaaaggctat aagttatatg taaactctct tatgagtaaa agcgaattgg
    11101 atgataatga taagattctt attgaacaat gtatgaatca taatataaat catataaaag
    11161 aattgattca tgaaacttct aagctattat cgcaactaac aaactataca actgtagcag
    11221 ttactaagag tttaataaat caaagtgtta taaagcacat acagttggtt gctatgaatg
    11281 acaataatat tttacttata gttgtaacag ataaagggga cttaaaaaaa gctaacctta
    11341 caactaatgt atatttagat caatctaagt taaatttgat ttctgataat cttacaagga
    11401 agctcttagg taaaagtata acagatttgg atgataatct aattgctttt attaaatacg
    11461 aaataagtga gtattctggg cttatagatg agcttttaaa tgctttgaac tcaaatatga
    11521 aagaagaaga tttttctcta tcattaaatg gagctactaa catatttagt tatccagaat
    11581 ttaatgatgt attaaaagca aagtcatttt taaatatgtt ggaaaaaaag gaaacaatag
    11641 ctgacataat taaatcaaag ggaatacaaa aagacaatct aaatataata attggtagtg
    11701 ataatgattg tgaactggca caggattgta gtatagttac agcaacttat aatgtagata
    11761 gagacttggt tgggcgaata agctttatag gccctaccag aatggattat gctagaattt
    11821 actctataat aaattatatg agtttattaa ttaatagaaa gtaaagaaat tttaaattgc
    11881 gaggtgtaaa gtcttggata aaaaaaatga gcaacaggaa gtaagagaag aaaatgatac
    11941 ttctattaac caagaatcgg aaactcaagt agagttagaa gaggaagttg ttaatgaaga
    12001 atgtgaaaca tcaagtgaaa aaactgatga aaaagaagtt gatgatgaaa atgtaacaga
    12061 tataaattct aagttagcag aaaaaaaatt gcaggatgag ttggatgaat taaatgataa
    12121 atatcaaaga cttcaagccg aatatgcaaa ctacagaaga agaactcaac aagagaaaga
    12181 gacaataggc gtatttgcaa atgaaaaaat aataactgaa ttgataccag ttatagatag
    12241 tatggaaaga gccttagatg catgtgaaga taaggaagat actatgtata agggtataag
    12301 ccttgtacat aaacaattaa tagacacttt agtaaaattt ggagtagaag aaatagaagc
    12361 agaatctaag gaatttgacc ctaatctaca tctagcagtt atgcaagaaa gtgtagatgg
    12421 agttgaagca aatcaaatag taatggtact tcaaaaagga tataagctag gaactaaagt
    12481 tgtaagacct tcaatggtta aagtttcttg ctaagtataa attaaaggtt aaattattta
    12541 acctaatata aaatattaaa ataagtaaaa cttaattata taattactcg aggaggattt
    12601 tagtcatggg aaaaataata ggaatagatt taggaacaac aaattcatgt gttgcagtgt
    12661 tagaaggtgg agaagcacaa ataatagcaa atagtgaagg tatgagaact actccatcag
    12721 tagtagcatt tacaaaagat ggtgaaagaa tagttggaga acctgcaaaa agacaagcag
    12781 ttacaaatgc agataaaaca ataacttcta taaaaactca tatgggaact gattataaag
    12841 taaatataga tggaaaatca tatactccac aagaaatatc agcaataata ttacaaaaat
    12901 taaaatcaga tgcagaaagt tacttaggac aaacagtaac agaagcagtt ataacagtac
    12961 cagcttactt tacagatgct caaagacaag caactaaaga tgctggtaga atagcagggc
    13021 ttgatgttaa gagaataata aatgagccaa cagcagcagc tcttgcttat ggtatggata
    13081 aattagacca agaaaagaaa atattagtat ttgacttagg tggaggaact tttgacgtat
    13141 ctatacttga aataggagat ggaacttttg aagttttagc tacagctggt aacaatagat
    13201 taggtggaga tgactttgac caaatagtaa tagattattt agcagaggaa tttaaaaaag
    13261 ctgaaggtgt agatttaaga aatgataaaa tggctcttca aagattaaaa gaagcagcag
    13321 aaaaggctaa gaaagaatta tcatcaacaa tgagttcaaa cataaactta cctttcataa
    13381 ctgctacagc agaaggacca aaacacttaa atatagattt atcaagagct aaatttgaag
    13441 aattaactag aggtttagtt gaaaaaacta tggaaccaac taagacagct ttacaagatg
    13501 caggactttc tacaggtgat atagatgatg tattattagt aggtggttct acaagaatac
    13561 cagcagtaca agaagctgtt aagaagttta taggaaaaga acctcataaa ggtataaacc
    13621 cagatgagtg tgttgcagca ggtgcttcta tacaagctgg agttttagca ggggatgtta
    13681 aagacttatt attactagat gttactccat tatctcttgg tatagaaact atgggtaatg
    13741 taatgactaa gataatagaa agaaatacta caataccaac taagaagtct caaatattct
    13801 caactgctgc tgataaccaa acagctgttg atatacatgt tcttcaaggt gaaagaagta
    13861 tggcttatga caatacaact ttaggaagat tccaattaac agatatacca ccagcacaaa
    13921 gaggaatacc tcaaatagaa gttacttttg atatagatgc aaatggtata gttaatgttt
    13981 cagctaaaga tttaggtaca ggaaaagagc aaaaaataac tataacatca aacactaatt
    14041 tatcagaagc agaaatagag caaaagataa aagaagcaga aatgaatgca gaagctgaca
    14101 aacaaaagaa agaaaaaata gaagcattca atcaagctga atctactata tatcaaactg
    14161 aaaaaacttt aaatgaactt ggagataaaa taagttcagg tgaaaaagag gatatagaaa
    14221 aagctatagc agacttaaaa gctgtaaaag ataatcaaga tgctacagca gaagaactta
    14281 agaaagctac agatgaagta atgactaagt tccaaaaagt atctcaagaa atgtaccaaa
    14341 aagcagctca agaacaacaa gcggcacaag gtgcagaaca agcacaagac aatggaccta
    14401 aagatgacaa tgtagtagat gctgacttta aagaagtaga tgaagataaa taagctatat
    14461 aatatgtaac actaaatgat aattggaaca tatatgattt gtgaaaatca tatagatgtt
    14521 aaagatatat ggacaatata catcataaag aatataatat atcatagatt atataactta
    14581 aagtagaata taagaattaa tgatagcttg tagtttatta ttctacaagc tatcgttttg
    14641 taaaatgaag gtggtgtcaa tttttgagta caaaaaggga ttattatgaa gtcttaggta
    14701 tcagtaaagg tgcagaagct caagagataa aaaaagctta tagaaaatta gcaatgaagt
    14761 accatccaga tagaaatcct ggagataaag aggcggaaga aaaatttaaa gagataaatg
    14821 aagcttatga agttctatct gatgatacaa agagaaaaac ttatgaccag tttggacatg
    14881 atgggctaaa tggtcaaggc ggttttggtg gtcaaggagg atttggcggt caaggctttg
    14941 gtggttttga agatatgttc ggagatatat ttggtgacat gtttggagga agcttcggag
    15001 gtggaagagc aagaagaaga gggcctcaac gtggtgcaga tataagacag agtgttacta
    15061 tatcttttga agaagctgca tttggtaaaa agatgtctat aaaagtaaat agaagtgaag
    15121 aatgtgaaga atgtaatgga actggagcaa aaccaggaac atcaaagaaa acttgttcaa
    15181 cttgtaatgg aactggtcaa gttagaactg ttcaaagaac tccatttggt aatatagcaa
    15241 gttcaagacc atgtagtgct tgtaatggaa ctggagaagt gattgaatct ccatgtagta
    15301 aatgtcatgg aactggaaat acaagaaaag ttaagactat agaagtagat atacctgctg
    15361 gtatagatga tggtcaaatg ataaaacttt ctggtcaagg tgaagtagga gaaaaaggtg
    15421 ctccaagagg agacttatat attgtagtta atgttaagtc acatccacta tttactagag
    15481 atggaaatga tatttatttt gaaatgccaa ttacatttgt tcaagcaact ttaggtgatg
    15541 agatagaagt acctactttg gatggaaaag ttaaatatag tgtgccagag gggacacaaa
    15601 ctggaactgt atttagactt aaagagaaag gaatccctag aattagaggt aattcaagag
    15661 gagaccaata tgttaaggtt gtagttgaaa ttcctaaaaa attaaacgat aagcaaaaag
    15721 aactattgag agaatttgcg aaggagtgtg ggtcgaacgt ccacgaaaag aagaaaacct
    15781 tcggacaaaa gatagaagat atgtttaaga aaaagtagtt aaagtagcca atatataatc
    15841 atatttttaa aatcttagtt atttataaga ttgtcaaagg agatattatg atttttatat
    15901 tggctatttt tgtataaaaa ataaatgtgc ataaaataaa actgaatata aataattttc
    15961 tgaaaaatac ttgtcctaaa atatataata tgctataata taatcaaata aaagaaaggg
    16021 gtgattccag tgggaacgga tgtggcactg ccaaatgatg aagaaaactt tattgtaata
    16081 tacaaaataa aattagatac ttttagtgct agattaagta ctggaaaaac tcatgagctg
    16141 ggataattat tgtaattacc tagactcaaa cttttagtag taaaatagta atattatata
    16201 gacaaggtat ctaattttta aatcttttgt atgaattaaa atttttataa agggtatatc
    16261 tgattagaat gtaattaggg aaacctagtt gtgaattact tttagtatat acatgtgtaa
    16321 agaaatatgt atatggtatt ggttattgtg ctagatgtgt atgtaaagga atatgtgtct
    16381 agttgggtat atgtaaagaa atatacttat aatctatgaa aaatttttag tataaattgc
    16441 taaaacattg agattgtttt ttatagaaag ttagtttata ccaactaaaa atgtgcttaa
    16501 gatttgtttg atgtttaatt ataaataagg ttgtagataa aaaaataagt aaagacaaag
    16561 gtttaaaata cttgctttaa atatagtttg ttttgaacac ttgccaaatt aaaataatta
    16621 ataaaataga catcttctca tagtaaaata tactgtgaga ggatgttttt gctttgttca
    16681 taaactttta tgaaaaattt gcata
//
LOCUS       DAYAOX010000081        16572 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.081, whole genome shotgun sequence.
ACCESSION   DAYAOX010000081 DAYAOX010000000
VERSION     DAYAOX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16572
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.081"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            212..349
                     /gene="rpmH"
                     /locus_tag="ACGI57_001976"
     CDS             212..349
                     /gene="rpmH"
                     /locus_tag="ACGI57_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFG9717054.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            427..771
                     /gene="rnpA"
                     /locus_tag="ACGI57_001977"
     CDS             427..771
                     /gene="rnpA"
                     /locus_tag="ACGI57_001977"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFG9717055.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            803..1054
                     /gene="yidD"
                     /locus_tag="ACGI57_001978"
     CDS             803..1054
                     /gene="yidD"
                     /locus_tag="ACGI57_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFG9717056.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            1086..1793
                     /locus_tag="ACGI57_001979"
     CDS             1086..1793
                     /locus_tag="ACGI57_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFG9717057.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            1762..2391
                     /gene="jag"
                     /locus_tag="ACGI57_001980"
     CDS             1762..2391
                     /gene="jag"
                     /locus_tag="ACGI57_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFG9717058.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            2503..3882
                     /gene="mnmE"
                     /locus_tag="ACGI57_001981"
     CDS             2503..3882
                     /gene="mnmE"
                     /locus_tag="ACGI57_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFG9717059.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            3977..5872
                     /gene="mnmG"
                     /locus_tag="ACGI57_001982"
     CDS             3977..5872
                     /gene="mnmG"
                     /locus_tag="ACGI57_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFG9717060.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRR
                     TRGGKGE"
     gene            5865..6584
                     /gene="rsmG"
                     /locus_tag="ACGI57_001983"
     CDS             5865..6584
                     /gene="rsmG"
                     /locus_tag="ACGI57_001983"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFG9717061.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKDEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            6747..7532
                     /gene="noc"
                     /locus_tag="ACGI57_001984"
     CDS             6747..7532
                     /gene="noc"
                     /locus_tag="ACGI57_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HFG9717062.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            7667..8440
                     /locus_tag="ACGI57_001985"
     CDS             7667..8440
                     /locus_tag="ACGI57_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9717063.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            8442..9305
                     /locus_tag="ACGI57_001986"
     CDS             8442..9305
                     /locus_tag="ACGI57_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFG9717064.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            9338..10480
                     /locus_tag="ACGI57_001987"
     CDS             9338..10480
                     /locus_tag="ACGI57_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFG9717065.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(10614..11189)
                     /locus_tag="ACGI57_001988"
     CDS             complement(10614..11189)
                     /locus_tag="ACGI57_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HFG9717066.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            11449..12741
                     /gene="rstA"
                     /locus_tag="ACGI57_001989"
     CDS             11449..12741
                     /gene="rstA"
                     /locus_tag="ACGI57_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin production/motility transcriptional
                     repressor RstA"
                     /protein_id="HFG9717067.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            12846..13448
                     /gene="yedF"
                     /locus_tag="ACGI57_001990"
     CDS             12846..13448
                     /gene="yedF"
                     /locus_tag="ACGI57_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFG9717068.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            13521..13784
                     /locus_tag="ACGI57_001991"
     CDS             13521..13784
                     /locus_tag="ACGI57_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFG9717069.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            13799..13984
                     /locus_tag="ACGI57_001992"
     CDS             13799..13984
                     /locus_tag="ACGI57_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFG9717070.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            14087..15283
                     /locus_tag="ACGI57_001993"
     CDS             14087..15283
                     /locus_tag="ACGI57_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9717071.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            15497..15775
                     /gene="rpsF"
                     /locus_tag="ACGI57_001994"
     CDS             15497..15775
                     /gene="rpsF"
                     /locus_tag="ACGI57_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFG9717072.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            15812..16246
                     /locus_tag="ACGI57_001995"
     CDS             15812..16246
                     /locus_tag="ACGI57_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9717073.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            16265..16492
                     /gene="rpsR"
                     /locus_tag="ACGI57_001996"
     CDS             16265..16492
                     /gene="rpsR"
                     /locus_tag="ACGI57_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFG9717074.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
BASE COUNT         6864 a         1757 c         3036 g         4915 t
ORIGIN      
        1 aatttagaat aaaattaaat agatttttac tagtattttc aataaaaaaa atacataata
       61 tatatatggt agattttgtg atataagcgt cccaaaaact cgttgacaac agtagtaaaa
      121 gtatttataa ttatatgtgt tatgtttact ttgagggtaa agttattatt tttataaacg
      181 gaaaataaaa tctgaaaggc ggtgcaaaat aatgagtaaa agaacttatc aaccaaaaaa
      241 gagacaaaga agtaaagaac atggtttcag aaaaagaatg aagacgtcta acggaagaaa
      301 cgtgttaaag agaagaagag ctaaaggaag aaatagatta actcattaat tacaaaaggg
      361 ccgctgcaaa ggtggccttt ttgtgtaaat aattacaatt catagctgtg tttatggagg
      421 ttaactatgg actttaatag gactaaaggg ttgaaaaaag actctgattt tagaaaagta
      481 tataaacacg gcaaatcttt tgcaaacaaa tatttagtaa tatatatact taaaaataaa
      541 tcagattata gtagagtagg tatttctgtt tcaaaaaaag taggaaaagc tattactagg
      601 aatagagtaa gaagattaat aaaagaagcc tacagattaa atattgatga aaaaattaag
      661 cctggatatg atatagtatt tatagcaagg gtatctagta aggatgctac ttttaaagac
      721 atagacaagt ctataaaaaa tttagttaaa agaacggata tttctattta attgtatgct
      781 ataaatatgg aggttaatga ctttgaataa aataatttat atattaaagg aaataagtaa
      841 gtatttatcg aatttatgta tttatttagt gagattttat caaaagtata tatctccctt
      901 aaagggacct acctgtagat tttatccaac ttgctcacaa tattcaatag aggcgtttaa
      961 gaaatatgga tttgtaaaag gtatgtattt aacgataaga agaattttaa aatgtcatcc
     1021 ttttcatcca ggaggttacg accctctaaa ataaaagaga gtaactatta ggaggtataa
     1081 aaaaattgaa tataatagga aatttcttag gattgattct taaggtaata tttgaatttg
     1141 ttaatcatta tggtatttct ataatacttt ttacgatact tgtaaagatt attttattac
     1201 cattaacaat taagcagact aagtcaacaa aggcaatgca agatatacaa cctcgtataa
     1261 aagagataca agaaaaatat aaaaataagc cagaaaaaca aaatgaagaa attgttaagt
     1321 tatatggaga agctaaaatt aatccactat ctggttgttt acctttatta atacagtttc
     1381 ctatattaat aggattattt agcgttttga gagaaccagt tgctcatggc gtttttgcaa
     1441 acaaagctgc atttttagca gctgacaatg ggtttttatg gataaaaagc ttaacatcac
     1501 cagattatgt attagcagta ttttcaggag caagtgcata tgtcatgcaa aaagtaatga
     1561 caccaaaaga tcagttacaa ggctctatga aagtaatgac atatgtaatg gcaggtatgt
     1621 ctttctattg gggatttata ttcccagcag gacttacatt gtactggaca gtaagtaact
     1681 tattctcaat agctcaatat tacttaataa tgaatccttt aaaagctaaa ctggctgcta
     1741 actctaagga ggaaaaagtt aatgagaaaa agcctaaaat taataaaaag taagagtaaa
     1801 gaggaagcta taagtaaagc tatagcagag ttgaatttaa aagcagagga tatagaagta
     1861 gaaatacttg aaaatccaag taaagggttt ttaggtttta taggtgctaa agatggtact
     1921 tatgaaattt ttgttatcga gagggaagta gatgtagcta aaaattttat agaggtaatg
     1981 ttgaaaaatg caaatgttga tgccaaagtc aatgtatctc aaaaagataa tttaatcatg
     2041 gttgatattg aaggcaaaga agctgcaagt ttaattggta gaagagggga aactttagat
     2101 tctattcaat ttttgactgg attagctctt aataaaataa ataaagattc tcatacaaga
     2161 gtacttgtag atatagaaaa ttatagatca aaacgagaag aatctttaat aagatatgct
     2221 aataaagttg ctagagaagt agcgaaaaca agaaaaacta aaaaattaga ttatatgaat
     2281 ccatatgaga gaagaataat acattcagct cttcaaaatg acaaatatgt aattacatat
     2341 agtgaaggaa ccgatccata tagaagatta gtaattgagt gtaaaagata aagtcaacct
     2401 cctaaacatt agggggtttt attttttaat attattaaat cattaatgat tggttaaatg
     2461 taaatctata tgtaaaaaat tatgaattaa gaggtgataa atttgtttat agatgataca
     2521 attgcagcaa tagctacagc acctggagaa ggtggtatag gtatacttag aataagtgga
     2581 gaaaaagctt tgaaggttgc agaagaaatc tttaaatcta tgtctggaaa gagtattgaa
     2641 gaatacaata agagaacatt aatatatgga aatatagtgg ataatgaaaa tataattgat
     2701 gaagttttat tggcatatat gaaaggacca aattcctata caggagaaga tgttatagaa
     2761 attaattgcc atggtggatt tatttcggta aagaagatat tagaattaat actatcaaaa
     2821 gatgttagat tagcagaggc tggtgagttt actaaaagag cattcttaaa tggaagaata
     2881 gatttatctc aagcagaggc tgtaattgat gttataaaag ccaaaactga tatagctcat
     2941 gaagttgctc aaaatcaatt agaaggttct ttatcaaaga agataagaga attgagagat
     3001 aaagtcactg agatattagc tcatgtagaa gttgctatag attatcctga ggaagatatt
     3061 gagcatataa catatcaaac tcttaaagaa aaaactgatg aacttaaaaa agacattaaa
     3121 aaattatatg atacagctga aagtggaaaa atacttagag aaggcttaaa gacggtaatt
     3181 gttgggaaac caaatgtagg taaatcttca ctacttaatt ctattttagg agagaataga
     3241 gcaatagtta ctgatatacc aggaactact agagatgtta tagaagagtt tgtaaacata
     3301 aaaggtatac ctttaaaaat tgttgacaca gcaggtataa gagatactga tgatattgtt
     3361 gaaaaaatag gtgttgaaaa gtctaaagaa tcatttacta gtgcagattt gatagtaatg
     3421 gttttagatg catctagaaa gttgtcagaa gaagatatag agatattaga aaaacttaag
     3481 gataaacaaa caatagtgct gttaaataag aatgatttga aacaagagat tgaagaagaa
     3541 aaaatactta aatatgtaga aaataatagt ataataaaaa tatcagcttt acaacaagaa
     3601 ggaatagaag aattgcaaga taaaatagag tctatggtat ataaaggtag tatcaaaaat
     3661 aattctagtt tagtagtaac aaattcaaga cataaagatg cacttagtaa agcatataaa
     3721 tctgcaacag atgctttaat tgctttggaa caaagtatgc catttgactt tgtagaagtc
     3781 gatttaaaaa atatatggga ctatcttgga tatattaatg gtgatacagt aacagaggat
     3841 ttactagata atatattcca taatttctgt attggtaagt aatgtacaaa agtaaacttg
     3901 tttttattat agagattttt aaaaaatttg aagtgtttct catgaaacat taattttgaa
     3961 aagaaaggaa ttataaatga taaagtttga agcaggaaaa tatgatgtta tagtagtcgg
     4021 agcaggacat gctggatgtg aagctgcttt ggctactgca agaatgggat ataaaacttt
     4081 gataataaca atgtctttgg attccatagc attaatgcct tgtaatccat caattggagg
     4141 gactggtaaa ggtcaattag ttaaagaaat agatgcctta ggtggtcaga tgggattaaa
     4201 tatagacaag acatatatac aaagtagaat gttaaatact gccaaaggtc cagcggtaca
     4261 ctctttaaga gcacaagcag ataaatttaa atatcatgaa gaaatgaaaa agacattaga
     4321 agatgaacca aatcttgata tagctatgga tgaagttgta gaaatacttc atgaaggaaa
     4381 tgttgtaata ggagttggaa ctaaactagg atgttcattt aaatctaaag ctgtaatatt
     4441 ggccactgga gtttatctga actcaaagat atatatgggt gaagttgcat tttatgaagg
     4501 tcctaatgca ttggggtatg caaaatatct tacagatagc ttagttgagc ttggccttag
     4561 aatgagaaga tttaagacgg gaactccagc aagagttcat agagatagta tagacttctc
     4621 tgtaatgtct ttacaagagg gtgatgaaaa agtaactcca ttctcattta tgaatgaaaa
     4681 tatagaaaaa aaacaagaac cttgttattt aacaagaact acagaagaaa ctcaaaaagt
     4741 tatactggat aatttaaaaa ggtctgctat gtatagtgga gtaatagaaa gtactggtcc
     4801 aagatactgt ccttctatag aagataaagt agtaagattt agtgataaaa catcacatca
     4861 attatttata gaaccagaag ggttaaatac taaagaaatg tatatacaag gaatttcaac
     4921 tagtttacct tttgaagttc aattagatat gtacaaaact ataaagggac ttgaaaattg
     4981 taagataatg agacctgcat atgctattga atatgattgt gttgacccaa ctcaactaaa
     5041 aataagtttg gaaataaagg gtgttgagaa cttatttagt gcagggcaat ttaatggaac
     5101 ttctggatac gaggaagcag cagctcaagg gcttatggct ggtataaatg cagttagaaa
     5161 gatagaagga aaagagccat ttgtattaga ccgttcagaa gcttatatag gtgtcttatt
     5221 agatgacctt gttactaaag gaactaacga gccttacaga atgatgacat caagagcaga
     5281 atatcgacta tatttaagac aagataatgc agatatgaga ttaactcaaa aaggttatga
     5341 tataggatta gttaaaaaag atagatatga aaggttccta aataaaaagg ctgctgtaga
     5401 gaaagaattt gaaagactaa aaaatgaaag agttacacca aaagaagtaa atagtttgtt
     5461 agaagaaaaa ggagcaacac caataaaggt tggaatatct ctatatgaat ttttaaagag
     5521 accagaggtt acttacgaat tgcttgaaga attagggaaa ggcgctggag aagatgtttc
     5581 aagagaagtt aaagaacagt gtgtaataat aactaaatat gaaggttata tagagaaaca
     5641 attgaaacaa atagaccaat ttaaaaaact tgagaataaa aagctggatg agaaaataaa
     5701 ttattcatct atagaaggtc ttagattaga ggctagacag aaactagatg atataaaacc
     5761 tatttcaata gggcaagcat ctcgtatatc tggtgtttct ccagctgata tatcagtttt
     5821 acttatatac ctagaacaaa tcagaagaac tagaggtggc aaaggtgaat aatatagaac
     5881 ttctgaaaaa cgggattgaa ggttttaata tagatacgaa tgacagtatg ttagaaaaat
     5941 ttaaaatcta tagagagata ttagttgact ggaataaaaa gatgaatctt actggaatag
     6001 aagatgaaaa agaagtgtat ataaaacatt ttttagactc tatttcagct gtaaagaatg
     6061 gatatataaa aaatgggatg tctataatag atgttggtac aggtgctggt tttcctggaa
     6121 taccattgaa aatatgttta gatagcttag aacttacttt gttagattcg ttaaataaaa
     6181 gaataaactt tttagaggaa gttagtagag tactagagtt agataatatt acatttatac
     6241 atggaagagc tgaagatttt ggtaaagatg aagactatag ggaaaaatat gatgttgcta
     6301 cggcaagagc tgttgctgga ttaccgattc ttatggaatt ttgtgtacca tttgtaaaag
     6361 ttggagggta ttttatttgc ttaaaaggtc cgaatgctaa tttagagcta gaagaatcac
     6421 aaaaggctat tgatgtttta ggattggagt atattgaaaa aatagatgta gagttaccag
     6481 aggtagattt aaatcataat atattagttt ttaaaaaagt gatggaaaca ccagttaagt
     6541 accctagaaa agctggtaaa ccagccaaaa atcctattaa gtaatgaaaa atttattatt
     6601 ggtaagctta attatttctc aggaaataat taagcttact gtaaaaaaac aaaaataaag
     6661 gaattattaa agtagatgca gaaataaatt tgtatgtaca tatagagaac aaaaataatt
     6721 tatatctaaa ataagagagg tatgttatgg aagataaaag agttatggag atacctatag
     6781 aagatgtagt tccaaatcca tatcaaccaa gaaaaatatt ttcacaagtt tcattggagg
     6841 aattgagcaa ttctataaag gtatatggta taattcaacc tataacagtt agagcaaaag
     6901 atggcaaata tgaactaatt gcaggagaaa gaagattaag agcagctaaa ttagcagagc
     6961 ttaaaaccat accagcaatt ataaataata tgaacgatga atcttctgct gttctagcgt
     7021 tattagaaaa tttgcaacga gaagacttaa atttcattga agaagcaatt ggttatgaaa
     7081 acttaataaa agaacatgct tttactcaac agcaattagc agaaaaattg ggcaaaaatc
     7141 aatctactgt agcaaataag cttagaatat taaagcttcc aaacgatata aaaatgaagc
     7201 taatagagaa taatttaaca gaaagacatg ctagagcatt tcttaaacta ccaagtgaag
     7261 atttaatgca aagtgtgtta gacaaagtca taaaaaatga attgactgtt aaaaaaacag
     7321 aaaaattgat acaagatgta ttagaagaaa ctaaagttca agaagagcct gataaaaaac
     7381 agaatataaa gggtgctatg agtataagaa tttacataaa tactataaag caggcctttg
     7441 atgctatatc aaatacagga attgatgcta agtataatga aatagataag ggagattata
     7501 tggaagttgt tgtaaaaatc cctaaaaaat agagattact gcttaccagt aatctttttt
     7561 tgtaatataa gccttgtaat ataaattgat ttatagacat tatttatact taaaaaacac
     7621 ctattcaaca tttaaaatta atttaattct taaagggggg aacatcatgg gcaaagttat
     7681 agctgtattc aatcaaaagg gtggagtcgg gaaaactaca acaaatgtaa atttaagtgc
     7741 tagcttagga actttgggaa agaaaatatt agtattagat ttagacccac aaggaaatac
     7801 aactagtgga tatggtatta ataaaaacga agtagaaaat acaatctatg aaattatgtt
     7861 agatgggtta catattaaag aagcaataat aagtactgaa tttgagaatg tagatgtagt
     7921 tccatcagct actgaactat caggtgcaga gatagagctt acatctaaaa ctaatagaga
     7981 gtatatttta aaaaattcca taaaagcggt aatagatgaa tatgattata tatttttaga
     8041 ttgtccaccc tcactgggga tgcttacaat aaattgttta actgctgtag atagtgtttt
     8101 aattccaata caatgtgaat attatgcttt agaaggtgtt agtcaattaa tggagactat
     8161 aaaattagta aaatcaagat taaatgctga tatagaaatt caaggtgttg ttttaagtat
     8221 gtttgatgga agagctaatt tatcaataca agtagttgaa gaagttaaga aatactttaa
     8281 aggtagtgta tatacaactt taattccaag aaatgtaaga cttgcagaag ctccaagtca
     8341 tggaaaacct gtaatttatt atgataaaag atgtagaggg tctgtagctt atttggagct
     8401 agcagaagaa tttatagatt tagaagagga ggaatggtag tatggagaat aagtctaaaa
     8461 gaagtaatag attaggtaga ggtcttagtg cattgatacc agaaattaaa ggagaaactt
     8521 cagaaaaaga aatagtcaac atagatatag acaaaatata tccaaacgaa gttcaaccta
     8581 gaaagcaatt tgatgaagaa aagataaaag tattgtcgga ttctataaaa aattatggtg
     8641 ttttacagcc aatagtggtt aaaatggatg aaaataataa atatatgata atagctggag
     8701 aaagaagatt tagagcatca aaacttgcaa ataaaaatca aatacctgct ataattaaag
     8761 atatagatat gaaagatata atggagatag ccttaattga aaacttacag agggaagatt
     8821 taaattcaat agaagaggct ctagcatata agagtttaat agagcattac aatgtaactc
     8881 aggaagaaat atcagaagct gttgggaaga gtagacctca tataactaat acactaagac
     8941 ttttaaatct tggacaagat gttatagaaa tgatagacag tggtagaatc acagcaggcc
     9001 atggtaaggc tttattgaga attgcagata aagatttgca acttcaaata gcgaaaaaaa
     9061 tagaagaaga agaactttct gtaagagaag tcgaaaatat agctaaaaaa atatctgaaa
     9121 ataaacaaga agaacctaaa aaatctaaac ctaaggatgt atttatttta gatgttgaag
     9181 ataaacttag aaatatattt ggaacaaagg tgaatatttc taaaggaaag aaaaaaggta
     9241 aaatagagat agaatactat aatgatgatg atttaaatag tattgtatca atgcttcttg
     9301 aatagaacaa aaatataaaa cagagaggaa gattgttatg atttatttag ataatgctgc
     9361 aactacatat ccaaaaccag aaagagtgta taatgctgtt ttagattgta tgaaaaatta
     9421 ttgtgcaaat ccaggtagag caggccataa acttgctatg agagctgcta gggagatata
     9481 tgatacaaga gagaatatag ctaagttgtt caatgtaagt aatcctatga atatagtatt
     9541 tacatctaat gcaacagatt ctttaaattt agcgataaaa ggtgtattac aagaaggaga
     9601 ccatgtaata acaacgtcta tggagcataa ttcagtaata agacctataa aagcattgga
     9661 aaaaaggggt attgaaaata ctgtagtaaa atgcgattat gagggatttt tagattatga
     9721 agatttagaa aaatcaataa agagtaatac taaactgatt gtaacaacac atgcgtctaa
     9781 tgtatgtgga actttaatag atataaaaaa agttggagaa atagctaaaa aacataatat
     9841 actattttta gtagatgcat cacaaacagc aggtgtttat gatatagacg taaatgaatg
     9901 taatatagat atgttggcaa tgccaggtca taaatgctta tttggacctc aaggaacagg
     9961 tatattatat gtaagagaag ggttgaactt aaatatatta aaagaaggtg gaacaggtag
    10021 taaatcagaa gaaatagttc aaccagagtt attcccagat aaatatgaat caggaactca
    10081 taatactcca ggtatagcag gtcttaacca aggtatacta tttatatttg aaagaggaat
    10141 caacaatata agacaacatg aagaagaatt atgtcaatat atgatagaca aattagagga
    10201 agtaccagat ataaagatat atggaccaaa ggacagtaaa aaaagagcat cagtaatagc
    10261 tctaaatata ggagatatgg attctggaga ggttactttc ttattagata gtgattataa
    10321 tatagcgact agatcaggaa tacattgttc accactagca cataccactt taggtacttt
    10381 aaaacaaggt gcagtaaggt ttagtatagg atatttcaac acaaaagatg agatagataa
    10441 agcagtggaa gctttgaaaa aaatttcaaa aaataaatag catgagattt aataatatag
    10501 aaataaaaaa aggggatatt tcaaaataaa aatttgaatc atctcctttt ttgtacaaaa
    10561 aagaaaaggg gttcctaaga acctctaatc tatttgataa tagtttcaag aaattacttt
    10621 aagtaatctc cttcttcaag aactatatga ccactttcgt aattagaacc atcaacagta
    10681 atttttattt gttcaacatt catatttttt atgtaagtac gagcaactga atcaagcatt
    10741 aaagtttctg catcactacc taagtttgac tttgtaaagt cagagctaac atctaatata
    10801 ccagttttta caccatcaat gtcttttgta gtaaatgaat ttaattttgc agattctgga
    10861 acaactttaa gtttttgaag ttcttcaaat aaagtgttct catcaatttt atttaaatca
    10921 actttatttt caattaaatc tgtcttatct acatcaaaag agtaaatagt agctgattga
    10981 gttttagttt cttcttttgg agtaggttta gctttgttat taggaacctc tttttcagta
    11041 ttgttttctt cttttggttg ctcaacattt gtattgctct tagtttcttt tttagatgaa
    11101 tcacttccat tttgagcatt agtacaacca actgcgaata tagatataga taatatactt
    11161 aacactaaca ttattttctt atttttcata aaaatttctc ctctcttaaa aactattctt
    11221 atatataatt atagtcgtta tgagcaaata tatgaataca aaatagtaac aaatttaaaa
    11281 aatgatttat ttaaattaat aaaactcaaa ataaactaaa ttttagtcat ttttatggga
    11341 aatcatttaa tgtacagtga aaatattgta aaataaaagt agtgttttta aaataaatat
    11401 aagttataat tatattataa aaaaatgtct tcaaaaggtg ggaatagtat ggaaattttg
    11461 agcttaggag aaaagattaa aaaattaaga aaagaaaaaa acatgacatt aaaagaatta
    11521 gctggagata ggataacagc agcacaaata agccatatag agagggataa atctcatact
    11581 agttatgaac tgttggaata tctaggcgat aagctagatg taagtgtaga atacttatta
    11641 gagaccaagg aaatgcaatc aaaaaagata actgataatt taatactaca gagcgaaatt
    11701 tatataaaag aaaatgaatt aaacaaggct gaggaccaaa taaaagaagt tttagatata
    11761 tgtaagagat acgatttaat ggagaactat ggcagatgta attttttaat ggctaatatt
    11821 aatttgaaaa aatctaatta taatgatgct atagaaaact ttgaaaaggc attgtacttc
    11881 tttataaaaa atagtgatag tgaaaatata cttaagtgtt atttaaacat agggaaaata
    11941 tatatgcagg aagactttta taaaggtgct ataagccact ttaattttgc agaggaaatt
    12001 ttgtcagaga ataaatttga ggatgttaac atacacaagg aactatatag taagatggca
    12061 tattgttaca taaaactaga taatccaaat atgtcattga agtacataga aaagataaat
    12121 gaaatagata atagaaatga tagaaaagaa gatgtcgata ttttggtgtt aaaagcaaat
    12181 aatatgcttg agattggaaa atatgaagaa tcaagagatt actttaaaaa agcactaaaa
    12241 atacttgaga gtgaggataa taaaactgga ttagcaaatg tgtatttaac aatttgtgat
    12301 gtttacagaa aaataggtga aacagataga gtacttgaat attcacaaaa ggtatatgac
    12361 ataaagaaaa atgatgaaga tgaatacatg atgtcaggat tatttaagat aatagaagca
    12421 tatatagata aagaagatta tgatttagct agaaaatatt gtaagatagc cttagcttca
    12481 tcaataaaaa ataaaaataa attcaatgaa tataaagcat tgagattcta tgctaatatg
    12541 tataaaaatc agaatgaagt aactcttgca attgaatatc tgattaaatg cataaaaata
    12601 gtatcagatt taggcaacaa tagaatattg gccaatttat atatagactt aggtcaatta
    12661 tattcaaata tatcaaaaga aaaagagtta gaatattatc aaaaaggtgt atttatgtac
    12721 aaaaatttag aaataatgta gggtaaataa acctataagg gtatagaaac aatctataac
    12781 aagcaagata atatctatga taaaatataa attgtatgaa tatttttaag gagatgaatc
    12841 agacaatgag tataaaaata gatgcgagag ggcttgcttg tcctaaacca gttataaata
    12901 caaaaagaga attggataac ctagaagaag gtgttgtagt tacaatagtc gataatgaaa
    12961 cagctaagga gaatatatta aaacttgcta aatctttaag ttgtgaagca aatattgttg
    13021 acgaaaaaga gaattttata tctatagaaa ttagaaaagg taaaaatata ctagataaac
    13081 aaaatgttgt agataataaa gaaagtgaat tagacaatac atgtatattt atatcttcag
    13141 ataagatggg acttggaaat gacgaattag gaaaagtgtt aataaaagga tttatatata
    13201 cattaacaga atctaaacct tatcctaagt atgtactttt agttaatgga ggagtaaaat
    13261 taagtgctga aaatgaagaa accatagaaa atctaaaaat acttgagaat atgggtgtag
    13321 aagttttatc ttgtggtact tgtttagatt attataattt aaaagacaaa ttacaagtag
    13381 gtacagttac aaatatgtat actatagttg agacattaaa gaatgcatca aataccattt
    13441 ctatataatg tatgtttgtt taataatata aaattaataa taaagattaa ctcaaataga
    13501 tagtcagaaa ggattaagta atgaatcaaa tgtatatagt gtcatttaat tcgacacatc
    13561 atgcgataag atcagaaaag ttatttggtg aaaatagtct aaaagtaatg gctcttccaa
    13621 caccaagaga aataacagca agttgtggaa tatcaattaa attttctttt gaagatatgg
    13681 aaaagataaa aactatttta gttgaaaata atgtagatat aaaaggaatt tattgtataa
    13741 gtaaattaga aaatggctca aaagaagtag agaagttaga ttaggaggct aattatttat
    13801 gccaatggat ttaaaaatag gtgatattgt tgaattaaaa aagcaacatg catgtgggtg
    13861 taaagagttt gaaatcataa gaacgggaat ggatataaag ataaaatgta ctaaatgttc
    13921 aaggctaata atgcttgata gagagacctt agaaaagaga gtaaaaaagc taataaaaaa
    13981 gtgataaaaa tttaacactt aaaaaatgtc aaataccgta tattaatatc caaaattttg
    14041 gtataataag aattgaagaa aacattttca tgggggtgct ttaattatgg cagttaaata
    14101 tgcaaaaaga atgcaaggat tacaaggatc agaaatacgt gagcttttaa aacttactca
    14161 acaaccacaa ataatatctt ttgctggtgg aatgccggct ccagagttat ttccagttga
    14221 ggaaatgaaa aaagtatcag ttgcagtgct tgaagaaaat ggaagatcag ctatgcaata
    14281 tactacaaca gaaggatatg aaccattaag agaaaaaata gcagctagaa tgaatgataa
    14341 aaacaaaact aatgtaaaca aagatgatat attagttaca agtggttctc aacaaggtct
    14401 agattttgca ggaaaagtat ttattgatga gggtgatgta attttatgtg aaagtccatc
    14461 ttacatagga gctataaacg catttaaatc ttatcaacct aaattcatag atgttccaac
    14521 agattctgat ggaatgataa tggaagaatt agaaaagata ctagaaacaa ctgatagaat
    14581 aaaaatgatt tatgtaatac ctgacttcca aaatccaaca ggaagaactt ggccacttga
    14641 aagacgtaaa aaattcatgg aaatagtaaa taaatttgaa ataccagtaa tagaagataa
    14701 tccatatgga gatttaagat ttgaaggaga gactttacca tctttaaaat caatggatac
    14761 aaaaggatta gtaatattct taggaacttt ctctaaaata ttctgcccag gatacagatt
    14821 aggatggact tgtgcttctc cagaaatttt atctaagttt aactttgcaa aacaaggtgc
    14881 agacttacaa gcatcaacta tatctcaaat ggaagtaagc aaatttatgg atatgtatga
    14941 cctagatgca catgttgaca aaataaaagc agtttatgtt aaacgtagag atgtaatgct
    15001 taagactatg gaagaagagt tcccagaagg attagtattt actcatccag aaggtggatt
    15061 atttacatgg gtagagcttc caagtaattt aaatgctaaa gaattaatgc caaaatgttt
    15121 agataagaat gttgcttatg ttgctggagg aggattcttc cctaatggtg gtagagaaaa
    15181 tactttcaga cttaactatt caaatatgcc agaagaaaaa ataatagaag gtataaaaaa
    15241 tatagcagct gtattaaaag aagctatggg tgtagaagct taattaaaaa taataactta
    15301 tatataatac aagggatggt tgttgccatc ccttgtttgt ttgaaaagta tataattcac
    15361 cttatactaa actttgtcca aaatttaaat tgacagaaat aagaataaat gataaaatgt
    15421 gtaggtatga gattttagtc tcatttctct gctctaagag tgtagagccg ttaagtccaa
    15481 agggaggtga attatagtga gaaattatga attagtttat gtggtaaagc caaactctga
    15541 tgaagaagta agagaggcta tactaaacaa agttaaggaa gttgttgcaa ctgacggaga
    15601 aatagttaaa gttgatactt ggggaactaa aaaattagct tatccaatag ctaagtttac
    15661 agaaggtttc tatgtattag ttaacttcaa atcagcagta gacgttccta aagagataga
    15721 cagaaactta aagataaatg aaaacgtaat aagacacatg atagttgttg cttaataact
    15781 ggttttatta taaaaaatag gtggtgtttt tatgaatcaa gttgtattag tgggaaggtt
    15841 gactagagac cctgaattaa gatacatccc aggtacagga acaccagtgg catcctttac
    15901 aatagccatt gatagagact atgtaaaaaa agatggatct aaagaaactg attttatacc
    15961 tgttgaagta atgggtaaat ctgctgaatt ttgtgctaac tacataacta aaggtagatt
    16021 agtagcactt caaggatcta taagagtaga caattatcag acacaatcag gtgaaaagag
    16081 aacttttaca aaagtaagta ctagatcagt acaagctttg gatagtaaga ataaatcgga
    16141 aaattcatat agagagagtg ctccagcttt tgagccaagc tttgaacctc aaggtttaga
    16201 cccacaaggt ttccaagcta tagatgatga cgatatacca ttttaaaaag gagggaaaaa
    16261 agtcatgatg aataaaaaaa gacgtaagaa aaaaagagtt tgtcaatttt gtgctgacaa
    16321 aaacgctaaa atagattaca aaagtactca aagattacaa aagtatataa ctgaaagagg
    16381 aaagatatta ccaagaagaa tatctggaac ttgtgctaag catcaaagag aattaacagt
    16441 tgctataaaa agagcgagaa atatagcact tttaccatat acattagact aaaaaaatga
    16501 gccttgtgct cattttttat tatatgtatt tatatatcta aatgaggcga gctataaagc
    16561 tcgttttttt gt
//
LOCUS       DAYAOX010000082        16233 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.082, whole genome shotgun sequence.
ACCESSION   DAYAOX010000082 DAYAOX010000000
VERSION     DAYAOX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.082"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..1305
                     /locus_tag="ACGI57_001997"
     CDS             <1..1305
                     /locus_tag="ACGI57_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFG9717075.1"
                     /translation="KIKLQRIAYTDNLTGANTIDKFVIDANKILCKNTQVKYALLYID
                     IDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEMFARISADNFIIIMKYIEEEDIT
                     KRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPEDRDINSIIDRANIPHKMAKGGHK
                     SSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIYLQPKIELNTGEIQGSEALVRWK
                     RPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIYLRKWIDAGINPVTVSVNVSRIH
                     LYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDNFDILIDIMNNLKKIGFLISMDD
                     FGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSKIIVTKVIEMAKELGMKVISEGV
                     ETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIVSNLVRG"
     gene            1481..1849
                     /locus_tag="ACGI57_001998"
     CDS             1481..1849
                     /locus_tag="ACGI57_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9717076.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            1851..2576
                     /locus_tag="ACGI57_001999"
     CDS             1851..2576
                     /locus_tag="ACGI57_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717077.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            2570..3334
                     /locus_tag="ACGI57_002000"
     CDS             2570..3334
                     /locus_tag="ACGI57_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717078.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            3556..4806
                     /locus_tag="ACGI57_002001"
     CDS             3556..4806
                     /locus_tag="ACGI57_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9717079.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            4997..5881
                     /locus_tag="ACGI57_002002"
     CDS             4997..5881
                     /locus_tag="ACGI57_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFG9717080.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            6243..6860
                     /locus_tag="ACGI57_002003"
     CDS             6243..6860
                     /locus_tag="ACGI57_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFG9717081.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            6968..9499
                     /locus_tag="ACGI57_002004"
     CDS             6968..9499
                     /locus_tag="ACGI57_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9717082.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            9933..10634
                     /locus_tag="ACGI57_002005"
     CDS             9933..10634
                     /locus_tag="ACGI57_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-G-Cd"
                     /protein_id="HFG9717083.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            10684..11766
                     /gene="vanS"
                     /locus_tag="ACGI57_002006"
     CDS             10684..11766
                     /gene="vanS"
                     /locus_tag="ACGI57_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFG9717084.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            12027..13127
                     /gene="vanG"
                     /locus_tag="ACGI57_002007"
     CDS             12027..13127
                     /gene="vanG"
                     /locus_tag="ACGI57_002007"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HFG9717085.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            13124..13930
                     /locus_tag="ACGI57_002008"
     CDS             13124..13930
                     /locus_tag="ACGI57_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFG9717086.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            13948..16086
                     /gene="vanT"
                     /locus_tag="ACGI57_002009"
     CDS             13948..16086
                     /gene="vanT"
                     /locus_tag="ACGI57_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HFG9717087.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         6180 a         1621 c         2902 g         5530 t
ORIGIN      
        1 aaaattaaat tgcaaaggat agcatataca gataatctta caggagcaaa tactatagat
       61 aaatttgtaa ttgatgctaa caaaatacta tgtaaaaata ctcaagtaaa gtatgcttta
      121 ttatatatag atattgataa atttaaatat ataaatgatt tatttggata tgaagttggg
      181 aatgaaattt tgcgtaattt aacaaagatt attaagagca atatatttga agaggaaatg
      241 tttgcaagaa tatctgcgga taattttatt ataattatga aatatataga agaagaagat
      301 ataacaaaga ggttaaaaac tatatttgaa gaattagact tgtttaataa taatcaagaa
      361 gaaaaatata agttagtttt aagttgtgga atctatttta tacttccaga agatagagat
      421 atcaactcaa tcatagatag ggctaatata cctcataaaa tggcaaaggg aggacataaa
      481 agttcatatg cattttatga taataagatt catgaccaag agataaaaga aaaagagatg
      541 gaaaatacaa tgttttcaag tctagaaaat aaagaattta ttatatactt acaacctaag
      601 atagaactta atacaggaga gatacaaggt tcagaagcac ttgttaggtg gaaacgtcct
      661 gataaagggc taatacctcc aaatgaattt atcccatttt ttgaaagaaa cggttttgtt
      721 ataaatttag atttatatgt gcttgaagaa gtatgtatat accttagaaa atggattgat
      781 gcaggaataa atccagttac tgtttcagtt aatgtttcaa ggatacattt atattgcaat
      841 aattttatag aaacatacaa aaatataatt gataaataca atatacctgc aaagtatata
      901 gaacttgaat taacggagag tatcatattt gataattttg atattttaat tgatataatg
      961 aataatttaa aaaaaatagg atttttaata tctatggatg attttggttc agggtactca
     1021 tcattaaata tgttaaaaga aattcctatg gatatcttaa aacttgacca aaaatttatt
     1081 atggaaactt ataatagtaa aagaagcaaa ataattgtta ctaaagtaat agaaatggca
     1141 aaagagttgg gtatgaaagt catatcagaa ggagttgaga cagaagaaca gtttaaactt
     1201 ttaaaagaag taaagtgtga tatggcacaa ggatatttgt ttggaaagcc aatgccaatt
     1261 gaagaatttg aacatttaat tgtatctaac ttggtaagag ggtaattaat ctgtaatata
     1321 aagaatttaa taaattgtgt taaaaatgga ctctaatgta gtgttcattt tttttgtaaa
     1381 attttttata cttttactaa aaagtaattg acatatactt tgtattatta tattattgta
     1441 ttaacaactt aatacaataa tataataagg aggcatgaac atggaatggg aacttgataa
     1501 taacaaacct atatatatac aattagtaga acatttaaag ttaaaaataa tttcagggga
     1561 aattaaaata ggatctaaat tagaaacagt gagagcacta gcagaagatg cggaagttaa
     1621 tccaaataca atgcaaaagg ctctaacaga acttgaaaga caaggtttag tttatagtca
     1681 gagaactaaa ggaagatttg taacagatga taaggaaaaa ataaaagcta tgaaagagga
     1741 aatagctaat gtggagataa atacattaaa agtaacctta gaaaagcttg gatatgatag
     1801 agatgaaatg ctaaaactta ttacagaaaa tctgaaagga gaattataaa atggataaaa
     1861 atataagcaa tgtttctcaa gatggaatag ttgaatttaa aggtgtaaac aagagttatg
     1921 gaactaagaa tgttttaaaa aatattgatt taaatatacc aaagggaaaa atagtcgggc
     1981 tgttaggacc taatggaagt ggcaaaagta caatgattaa gctaatgaat ggattattac
     2041 agcctgataa tggtgaaatt atgataaatg gaatgaaacc atccatagat actaaaaaaa
     2101 tagtttcata tttaccagaa agaacttatt taaatgattg gatgaaggta tcagatttac
     2161 ttaagttttt ctatgatttt tatagtgatt ttgatgttag aagagcaaat gagatgatta
     2221 aaagcttaga tattgatgta aatgaaaagc taaagacaat gtctaaagga actaaagaaa
     2281 aagttcaact tattttagta atgtcaagaa atgcgagcat atatatattg gatgaaccta
     2341 taggtggtgt tgacccagct gcaaggtcat atatacttaa gactatatta aaaaattatt
     2401 ctgaagatag tactttactg attgctaccc acttaataag tgaaatagaa aacatatgtg
     2461 atgagataat attcatatca aaaggagaaa tagtgttaca aggagacgtt gaagcaataa
     2521 gagaagaaaa aggaaaatct atagatgcat tatttaggga ggaatttaaa tgttaggtaa
     2581 attattaaaa tatgagttaa aagcaagtgg aagaatattt ataccgttat atatagccat
     2641 attgatagta gctgtattta atggaatatt tatgaataca aatatacttc aggtacaggg
     2701 aataggaata ctagtattaa caagtctttt tatggcatta ggagttttaa ctattgtagt
     2761 tacaattcaa aggtttagaa aaaatctttt gggggatgaa ggttatttaa tgtttactct
     2821 tcctgttagc actagttcac ttatattatc taaatgtata acagcactta tatacgctgt
     2881 acttagtttt atagttgcag tattcacttt tggagttctt atgctttttg gtacttctgg
     2941 aattttacta cctgaaattt tagatttgtt taacactagt tttaaatgga tttctgaaaa
     3001 ctttttagat attttattat tagtagtagt aatgtttatt tcatattcat catttatttt
     3061 gttattatat acatcaatct caatgggaca attgccaaaa ttcaataaac atagaaacat
     3121 agtggcattt gcttctttta tagctataaa tatagtaata tcaattgttg gagatgcagt
     3181 tggaagcatc ttaccaaatg aagatacaaa tatggtatat catctttatc agagtcctag
     3241 ttttatgctt gcaatacttg gtagtcttgt agttgcaata gcgttatttt tcgctacaaa
     3301 atttatatta gataaaaaat taaatttaga ataaatgttt ttatatggag ctatcttttg
     3361 agtaatgtta aaagatggct cttattattt atgtagttaa ttcatgtata taatatgaat
     3421 agtgttttat ttctagttaa atatatacat atgttgtata atgatattgc gtgtaaaaat
     3481 ttgtaagaat aactatacac tttatataag ttatatcaag aaaatttaaa aatatatgga
     3541 tttgagggag aagatatgaa aagttataca aaggctaagt ggagtgtttg gggtataatt
     3601 acattttcat ttgttttagt attattttta aggatgtcaa ctgctgttgt ttcagacaat
     3661 ttagcaaatg agttaggttt taattctata caaatttcaa atatagcatc cttttgccta
     3721 tatgcatatg catttatgca aatacctgca ggaattttga ttgataaata tggagcaaga
     3781 aaaattagca gtcttggaat aataatggca agtttagggt caattttatt tggtttgatt
     3841 caaagtatag aattagcata tatttctcgt gtaattgtag gggcaggaac atcagtaata
     3901 ttactttgta ttttaaaaat tcaagggaga tggtttaata agtcagaatt tgcttctgca
     3961 acagctaaat tttcatttgt aggaaattta ggtggagttc ttgctacttt tccacttgtg
     4021 tttttgtctg aacttgttgg ttggagaaat tcatttttat taatcggtat aattggtgtt
     4081 gtaattggat gttttatgta tattattgtt agagatacac caaaagagta tggattcaat
     4141 gtagatactg aaccctatga aaagtcagag aaggttaata ttgttgatgg tattaagtct
     4201 gttataaaga acaaatcaac atggtataat tcaatgataa tgttctcttt tgttggtctt
     4261 acaagtgcat ttataagctt gtggggagta agatacataa tggacgttta tggtgtgagt
     4321 aagagttttt ctgcgtttat agtatcattt tttacatatg gatttatatt tggttcaata
     4381 attatggatt ttgtatttgc taaaataagg tctagcaaat ttaatataat aaaatttgga
     4441 gcaatgatag atttgtttat atggattgta attgtagttg tataccaggt aaaaccacca
     4501 attatagttt taccaatatc attctttatt atgggatgta tagttatgtc acatctacag
     4561 gtttttaatg atgccaagta taaaaataaa gaaatttatt ctggactggc aactagtgtt
     4621 attaatactt ttgaatttat aggaagtgga attattaatt tgattatagc tatctcttta
     4681 caagtaaatt catataatat agttgatgga tataaaaaag gttttgtagt atttatagta
     4741 ctaagtatta taactatagt atcatctcat ataggggtaa aaaatgatga ttttaaggct
     4801 atatagtgtt ttattaatag tttaatttag tattatattt tgcatatata taacaaatag
     4861 tttgcatttt cctaatatgt ataattggaa tataaatctt gtttactatg tacaaatggt
     4921 attgtattat attaaataga aaataagaac tttaaactat aatattggtt ctaaaatata
     4981 aggtaaggag ttttttatgg atacgacgaa aatagatgat ataagcaagt tagatttaaa
     5041 taatttagat attaataaga tggatatggg aggtataatg gaaagtctgc tagaatgggc
     5101 tactacaagc ggagtaaaac ttataatagg catacttata ctttctattg gatttaaaat
     5161 aataaaaaaa tttgtaaacc atgttatgtt attacttagt aaaagagata ttgatttaac
     5221 tttaagaaag tttttaaagt ctcttctttt gagtgtactt aaagttgccg ttataatcat
     5281 tgttctggaa tattggggaa tgagtttgtc cagttttgct gctgtgatag catcagcagg
     5341 ggtagcaata ggtttggctt tacaaggtag tctatcaaat tttgctggag gatttattat
     5401 actattgatt agacctttta aagtaggaga ttacgtagaa gcagcaggtc atggaggaac
     5461 ggttgaacaa ataggtcttt tttatacaca attggttact ccagataata aacagatact
     5521 tatacctaat ggttctgtat caaatgatag tctaataaac tattcagcaa agaatactag
     5581 acgagttgat ttgactttta gtgtagggta tgaagatgat atactacatg ttagaagagt
     5641 acttaaagat attgttaata atcataaatt aataataaat gaaccagaac catttatagg
     5701 agtagtagaa catggagata atgctattaa atttgcaact agagtttggt gcaagacaga
     5761 agattattgg acaatttatc ttgatttact agaagaggtt aaagttaggt ttgacgaaga
     5821 aggaattacg attccatatc ctaaaatgga tttaacggta aaagaattaa acaaaattta
     5881 attttttaat atagtgttta aatttaagaa tatcatatag atatacttat aaggtttgta
     5941 aaaaattaat aagtactgtt attttgtaag taaaaatata aaaaaatggg agtgtctcaa
     6001 aatgaacttt ttagttcatg aggcactctt tttgtacgaa ataaaatata tttttattat
     6061 ttcaacaatg aaaaagaggc ttgtttccat tttgagacag tataattttt atgtaacttt
     6121 aattttagta aatataaagt tttaaaattg ataaaatagg aggaattttt aatataaaag
     6181 ggtaaataaa gggtttagag ggcataatat agaataaatt aatatattta aggagggttt
     6241 ttatgtgtaa aaaaacgtat ttattagtgg taagtatgct tatagcgggt ttattaactg
     6301 catgtagtgg gagtccaact aaggattcag gacaagcaca agataataca aaaaaggagg
     6361 caaatgcatc aaataacgct ttaaaagatg aaaaaaataa tgaaaattta atggaacaag
     6421 attttaaagt gccatacaca gatgcaataa acatatttaa agataaatat aaggatgctg
     6481 atatagtaga tttaagtctt gagagagatt taaataaatt tgtttatact gtggaaggtg
     6541 tagatgataa taatgaatat aagatgaaaa ttgatgctaa tacaaaagat gtattagaag
     6601 ataaaacaga aaagttggat tcagaagatt taaatggagt tgctagaaaa gaaaaattag
     6661 atttaaatga cataatgaca cctcaacaag ctatggaaat tgcattaaaa gaacaaaatg
     6721 gaatagttaa agaatggagt ttagacaaag atttagatgt tactttttat aaaataagaa
     6781 tagataaaga taaaaatgaa tatgacataa aagtagattc taaaaaggga actgtattga
     6841 aggtagaaaa agaagattag aagatttaaa ctatctttaa aataatttct tttttgaata
     6901 aattttgttt aaaatataac aagcagggta taaagagact aaataactat tttaggagga
     6961 gtacggtatg tcattgaaat cactaaaaat aaaagaaaat ttatattggg taggttcgct
     7021 tgaccctgat ttaagagtat ttgacataat tatgtataca ccttatggaa ctacttataa
     7081 ttcatatgta ttaaaaggaa cagaaaaaac agttttattt gagacagtta aagataagca
     7141 ttttgacaat tatattgaaa gattaaatga tttaaatata gattttgaaa aaatagacta
     7201 tattgttgta agtcatactg aaccagacca tgcaggaagc gttgaaaagc ttttggattt
     7261 agctaaaaat gcaaaagtag tagcttcaga aacagcaatt aaatatctta aggaaatcgt
     7321 aaacaaagac tttgaatatg ttgcagttac agatggagat acattatcaa ttggggacaa
     7381 aactttagaa tttttctctg tacctatgct tcattggcca gataccatat acacatatat
     7441 aaaagaggat aaaactcttg taacatgtga ttcatttggt agtcactata gtaatgataa
     7501 gatagtaaat acacttgatg aaaatgaaga aaaggattac ttggatgctt taagatacta
     7561 ttatgattgt ataatgggtc catttaaacc atctatggta actgctatag aaaagataaa
     7621 agatttagat atagatactg tatgtccagg tcatggtcca gtattaactg aaaatcctag
     7681 aaaaattata gacctttatt acaactggag tgtaaatgaa caaataaagt tagaaaaaga
     7741 agttacaatt tgttatgttt ctgcacatgg atatacaaag attatggcag aagctataaa
     7801 agcatatatt gaaaaaaata gcaactataa agtaaattta tttgatgtaa tagagcataa
     7861 gcaagaagat attttggcaa aaatagcagt atcacaaggt gtattatttg gaacaccaac
     7921 aatattagga gatgcgttaa agcctatatg ggatatatta atatcgttaa acccagttct
     7981 tcatggtgga aaagttgctt cagtatttgg ttcatatgga tggagtggag aaggtataga
     8041 aaatgctatg gagagaataa gtcagcttag aatgacagca gtaaaacctt ttgctgttaa
     8101 ctttaaacca tcaaatgaag agattgataa attatattct tatacaggta aattcctaga
     8161 taagttaaat tctacttttg gaagtaaaaa gaaaactaag aaatttaaat gtgtaatttg
     8221 taatgaagtt tttgaaggtg atagtgctcc tagtgtatgc cctgtatgtg gagctaagga
     8281 agaccaattt atagaagttg aagaagatga agttactttt agaaaagata ctgatgaata
     8341 tttcgtaata gtaggaaatg gagcagctgg tttttatgca gcagatgcta taagaaaaag
     8401 aaataaaact tgtaagataa ctatgatttc taatgaagat gagttgactt actatagacc
     8461 tgcactttct gatggtataa atgaagaact tggttcagat ttctacatgg aagataaaga
     8521 ttggtatgat aaaaataata tagtagtaat attaggaacg aatgtagata aattagatga
     8581 agtgaataaa actattatag tgaatgatgg agctattaaa tttgataagc ttgtaatagc
     8641 tacaggaagt agaaacttta taccaccaat taagggccat gatttagaaa atgtgtttac
     8701 tcttagaaat ataaaagatt tatatagtgt aaaagaagca ctagaaaaat ctaagaaagt
     8761 tgtagtgatt ggtggcggtt tattgggact tgaagctgca tgggaattta gactaaaagg
     8821 attagaagta gtagtagttg aagccatgga tagcatacta tcaaaacaac ttgataaaga
     8881 aggtagtaaa atattagaac aatgtgtaag agatacagga atagatgtaa gattgggtgt
     8941 agcagttgat ggtattgaag gtgatggaaa agctcaaaaa gttgtattta aagatggaga
     9001 tagtgtcgac tgtgatatgg tagtattttc aattggtgta agagcaaaca ctcaaatggt
     9061 tcaggataca tctgtaaaaa ttgatagagg tattgttgta gataagacat tacagacaaa
     9121 tgttaaagat atatatgctt gtggagatgt tgcacaagtg ggaaatatta gtttagcaat
     9181 atggccatct tcagtagaaa tgggaaaaat agcaggggca aatgcaagtg gagataattt
     9241 gacatttgaa agtgaagtat atccagtttc attagatgca atgaatgtaa aagtatttag
     9301 tattggtaat atacaaaatt ttgataaaga aatttcatca aaagatgaag gtcaaagaat
     9361 atataaaaaa ttatttatga aagatggttc attagttgga gctatactta taaatgattt
     9421 aagctgtact gttaaattga taagattaat atctgaaaaa ggtgattttg aggatattat
     9481 gaaggctgat atactataat ttaaatacat taaaataaaa aggagttgtc tcaaaattga
     9541 tttttttcta atcaaatttt aaattcaact ccttttttga atttttatat aaaataacat
     9601 ggaaatttta gtaatgtctg tggttacata cataggttta attgtttttt atatggattg
     9661 tctctggtat aataaattga attgtgtttg actgatagat atttttattg aaagttaata
     9721 cctgttatgc gataataagg acgtgttagg ggagttttta taataacaat taatctcagt
     9781 ttttgtgttc caatagtagc atcaacacat tctgatgtag tagttctttt cttataaaaa
     9841 ttagttacaa atatgcttgt aattatttga tatgtaaatt taccaatatt ataaatgatg
     9901 acctcgataa taaggagagt gaaaaataat ctatgaacga aaatattttg attgtggatg
     9961 atgaaaaaga aatagcagat ttaattgaag tatatttaaa aaatgaaggt tttacagtat
    10021 acaagtttta tacaggtaag gaagctctag aatgtattga atctaaaaat ttggatatgg
    10081 ctattttaga tattatgctt cctgatattg atggatttca tatttgccag aaaattcgag
    10141 aaaaatacta ttatcctatc attatgttaa cagctaaaat tgaagatata gacaagataa
    10201 tgggtttaac cataggtgct gatgattata taacaaagcc atttaaccct ttggaagttg
    10261 ttgcaagagt aaagacacaa ttaagaagat atatgcgata taataattct tatgaacaac
    10321 agtcgataat tgttaatgag tatgatatta aaggattgat tatcaataaa gaaacacata
    10381 aatgtagttt atttgggaaa gaagtagcat taacaccgat tgaattttct attctttggt
    10441 atttatgtga acatcaagga aaagtggttc cttctgaaga attgtttgaa gctgtatggg
    10501 gagaaaaata tcttgataat aataacacag ttatggctca tatagggcga ttacgtgaaa
    10561 aaattcatga accttctaaa aacccgaaat ttataaaaac tgtatgggga gtaggttata
    10621 caattgaaaa gtaaaaatat taaagtcaat cctgacttta ctcaagtgag aaataaaatt
    10681 tttatgagaa cagctttaat ggcatttata gccattattt tcatatggat tttatattct
    10741 tttatattgt atggtaagtt ttctatctgg atagtatcat tctttgaaaa tgcttttcgt
    10801 atggaatata aagctgcatt gaatttatat cagcagattt tccgtaatag catggagctc
    10861 ttcatgatac tagctatggt gattgtgttt tttataatat tccgggttta tttaaattgg
    10921 tttacaaaat attttagtga aataaatcaa ggtattgact ctcttatcaa agaagatgtt
    10981 ggagaagtgg ctttatctcc agagttattg gcaatagaaa agaaaataaa ctcaattaaa
    11041 catatacttg aacaacgtaa atttgagaca caaatggcag aacaacgtaa gaatgaactt
    11101 attgtttatc ttgcacatga tttaaaaaca ccacttacat cagttatagg gtatctgaca
    11161 ctacttcgag atgaaagtca gatttctgaa gaactgcgaa aaaaatattt atctatttcg
    11221 ttggataagg cagagcgtct tgaagattta attaatgaat tctttgaaat tacaaggttt
    11281 aatttatcaa atatatcact tgaatacagt acagtaagct taactcgtat gcttgaacaa
    11341 ctaacatatg agtttaaacc tactttgatg agtaagaatt taaaatatac actgaatgtt
    11401 acaccaaata tgatgataag gtgtgatgtt aataaaatgc aacgtgtatt tgataactta
    11461 ttacgaaatg ctgtttatta tagttttgaa gatactacta ttgaaataac agctgtacag
    11521 gaggatgatt atataaagct taaatttata aatcatagca atacgattcc agaggaaaaa
    11581 cttgagcgta tttttgaaca attttacagg ttggatacat ctcgtaattc aaacagtggt
    11641 agagctgggt tgggacttgc tatagctaaa gaaatagtgg agcttcataa cgggacaata
    11701 acagctcata gcgagaacaa tataattatc tttaaggtta ttattccgat tgtaggaaat
    11761 ttgtaagaat ttaagtataa aaaaatagga aattctttag gataaataaa taaagagcac
    11821 tcttgttatt gatataatta ctgtatcaaa acaagagtgc ttttattttg atacagtaat
    11881 tatatcaata gaaaaatgtg aaaatatatt atgtggaaca gatgcaattg taagtgaagg
    11941 ttgattggat ttgaaataga aaaagtattt taacatgttt ctgtagattg tataaaaata
    12001 aataaatcaa caaaggagaa tcaaagatga ataataaaaa aattgcagtt atatttggtg
    12061 gaaactcaac agaatatgat gtatcattac aatcagcttt tgcagtactt gagaatttga
    12121 atacggaaaa atatgatatt ataccaattg gaattacaag ggagggtaat tggtatcatt
    12181 atattggaga ttataataat atccagaata acacttggtt tgaaagggat actgatataa
    12241 ttccagttat tgtttcgcag aaccgtgtca aaaatggtat tattgaattt ttaattcctg
    12301 atgctgtttc tcagaatcat attccaaata gtgctattga atcatgtgaa aatgatatta
    12361 aatacactaa acttgatttt gtgtttcctg tactgcatgg aaaaaatgga gaagatggaa
    12421 cagtgcaggg gttatttgaa ttggcaggta tacaggttat aggatgtggt actctttctt
    12481 cagcattatg tatggacaaa gacaaatcac ataaacttgt tcattcagaa ggtattaaag
    12541 taccaaaatc aatattacta aaatactcag ataaatttaa tgtggctgaa ctgtcaaaag
    12601 acttaactta tccattattt ataaaacctg tgcgtgcagg ttcatcattt ggtattagta
    12661 aaatttataa aaaagaagag ttacaagtag cagtagactt ggcttttgaa catgataatg
    12721 aagtaataat tgaggaaaat attgatggtt ttgaggtagg gtgtgcagtt ttaggaaacg
    12781 atgacttact tgttggaaga gtagatgaaa tagaattgtc aaaaggattt tttgattata
    12841 cagaaaaata tacattgaaa agttcaaaaa ttcatatgcc agctagaatt gatttggatt
    12901 gtgagaaaag aatacaagag acagcaaaaa ttatttatag aactcttgga tgttctggat
    12961 ttgctagagt agatatgttt ttaactccat ctggtgaaat tatttttaat gaagtcaata
    13021 caattcctgg ctttacttct catagtcgtt atccaaatat gatgaaaggt attggattgt
    13081 cttttgaaga aatattggac aagataattg aggtgtacat aaaatgatga ataatatttt
    13141 attagataaa aataaagaag ataaaatgac aaatactatc ttattgacta aagataaagt
    13201 ttatgatggg aatctaattc tagtaaatgc ttttttacca gttaaaacat ctgaagatat
    13261 agatttaatt cctgtagata caaggtttcc aagtattctt atgaaacgtg aagctacaaa
    13321 cattttacaa aatattttaa aaagtatatg tggaataaat gaaatagtac ctgtaagtgg
    13381 atatcgtact gcagaggaac aacaagatat atattcaagt tctctgcgtg acaatggaaa
    13441 agactttact aaaaagtttg tagcacttcc aaatcacagt gaacatcaga cagggcttgc
    13501 tattgattta gggttaaaga aagaagttat agattttatc tgccctgaat ttccttataa
    13561 tggtatttgt aatgagtttc gtaaggcagc actacattat ggttttatag agcgttatca
    13621 gtgtaaaaaa gaagaaataa caggaatatc tcaagaacca tggcattttc gttatgtggg
    13681 atatccgcat tctgagatta tcaataaaat gaatctcgct cttgaagaat atatagaata
    13741 tattaagaga ttttcgtata caaattgttt aaaaatagaa gataaagaaa aaattataga
    13801 aatcttttat gtgcctattc atactaaaga agaagtgaga atctctatac ctgaacaatg
    13861 tgtatatcaa atatcgggta acaataagga tggttttatt gtgacatttt ggagaaatag
    13921 aaaggactaa gaaatggagg aacaagaatg attaggaata aatcttatgc agggcttgat
    13981 tattttagga ttatagctgc attacttata attgcaattc atacatctcc attattatca
    14041 tatagtaaaa tggctgactt tgtgttaact agtataattg ctcgtattgc tgtgccattc
    14101 ttttttatgc tatcaggctt ttttctaatt tcaacatata attataattg taataaactg
    14161 aaatcttttc taaaaaatgt attatttatt tacgggatag ctattgtaat ttatattccc
    14221 ttaaatattt acactggata tttttccatg aataatttag tgcccaatat tattaaggac
    14281 attgtatttg atgggacttt atatcattta tggtatctac ctgcatcaat tattggtgca
    14341 agtattgcat actttcttgt aaaaagacta ggtttaaagt actcactagt aatcactatt
    14401 gttatttatg taattggtat gtttggtgat agttattatg gttttttaga aaaaatacca
    14461 ctattaaaga atttatatgg gtatattttt gaagtatcag attatacacg aaatggtata
    14521 ttttttgcac ctgtattttt catacttggt ggaataatat ctaataaatc taattatatg
    14581 tcaactaaaa atagcttgat tggatttctt atatcattct tacttatgtt atgtgaggga
    14641 atgtttttac ataaactagg ggtacagcgt catgatagca tgtatattat gttattgcca
    14701 tgtatgtatt tcttatttac agccttaaca tttttgaaag gttctcgagc taggttattg
    14761 cgaacatcat cattaattat ttatattata catcctatga tgattgttgt tattcgtatg
    14821 ttttctaaaa tattcggaat acaaactgtc ttggttgaca atagccttat tcattatata
    14881 gtggtaagta tagcatcttt tatagcttct ataattatta tattgattct taaacctcta
    14941 aaaaaagact tgaaaggaga acataaggta aataccaata gagcttggat tgaaattgat
    15001 ttaaataatt tggaacataa tgtaaagatg atacaaaaag ctatgccaga agattgtgaa
    15061 ttgatggcag ttgtaaaagc taatgcctat ggtcatagtg cttttgaagt agcgacttgt
    15121 gcaaaccaaa taggtgtaaa agcatttgca gtagcaacaa tagatgaggg gattgattta
    15181 cgtcgatatg gaatactagg agaaatattg attcttggat atactaaccc aataagggca
    15241 aaggaacttc ataaatacaa tcttacacag agtgttattg accttaatca tgctatctct
    15301 ttaaacaaac agaaatacaa cataaaatca cacattaaaa ttgatacagg tatgcacaga
    15361 ctgggttttg atgttaatga tgtgaggtca attttaaagg catttaaatt aaaatactta
    15421 gatatatgtg gcatatttac acacttgtgt gtttcagata gtctgaaaga tgaagatgta
    15481 gactttacaa ataaacaaat tgaatgtttt tataagttaa ttgattttct gttaaaaaaa
    15541 ggtataaaaa ttcctaaagt acatattcaa agtagttatg gtttttttaa ttatcccaat
    15601 cttaagtgta attatgtaag aattggaatt gcattatacg ggatattaag ttctccaaat
    15661 gatacgacta aattgcaact tgatttacga cctgttttat ccttaaaatc acaagtaatt
    15721 cttattagaa aaatacaaaa agatgatagt tttggatatg gaagagtctt tattgcaagc
    15781 agagatagcg taattgctat attatctatt ggctatgccg atggtatacc tagaaattta
    15841 tcctgtggaa aaagttacgt aataataaat ggttgtagag cagcaatagt gggtcgaatt
    15901 tgcatggacc aacttgctgt tgatattaca gatataccta atgtagaagt tggtaatacg
    15961 gcaataataa taggaagaga taatttatct gaattatctg cttctgaagt tgcaaataac
    16021 tcatgtagta tatcgaatga attattgagc agagtaggaa gaaggttaaa tgttataaag
    16081 aagtaactca attaaatagt aatatttgtt gtactgtgta atttatttta ctctgactag
    16141 acatttttag taatattatg tctaatatat attttttata acttacatga caatttaagt
    16201 tataaaaagg tgatgtttca gatttttatt ttg
//
LOCUS       DAYAOX010000083        16178 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.083, whole genome shotgun sequence.
ACCESSION   DAYAOX010000083 DAYAOX010000000
VERSION     DAYAOX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.083"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            55..771
                     /locus_tag="ACGI57_002010"
     CDS             55..771
                     /locus_tag="ACGI57_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HFG9717088.1"
                     /translation="MKLDLFLLAIIPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            850..2160
                     /locus_tag="ACGI57_002011"
     CDS             850..2160
                     /locus_tag="ACGI57_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFG9717089.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            2260..2796
                     /gene="lepB"
                     /locus_tag="ACGI57_002012"
     CDS             2260..2796
                     /gene="lepB"
                     /locus_tag="ACGI57_002012"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFG9717090.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            2962..3498
                     /gene="lepB"
                     /locus_tag="ACGI57_002013"
     CDS             2962..3498
                     /gene="lepB"
                     /locus_tag="ACGI57_002013"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFG9717091.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            3508..4263
                     /locus_tag="ACGI57_002014"
     CDS             3508..4263
                     /locus_tag="ACGI57_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFG9717092.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EILTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            4337..6004
                     /locus_tag="ACGI57_002015"
     CDS             4337..6004
                     /locus_tag="ACGI57_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HFG9717093.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            6971..7900
                     /gene="glsA"
                     /locus_tag="ACGI57_002016"
     CDS             6971..7900
                     /gene="glsA"
                     /locus_tag="ACGI57_002016"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFG9717094.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            7998..8561
                     /locus_tag="ACGI57_002017"
     CDS             7998..8561
                     /locus_tag="ACGI57_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9717095.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            9049..9885
                     /locus_tag="ACGI57_002018"
     CDS             9049..9885
                     /locus_tag="ACGI57_002018"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008833 - deoxyribonuclease IV
                     (phage-T4-induced) activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFG9717096.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            10169..11164
                     /locus_tag="ACGI57_002019"
     CDS             10169..11164
                     /locus_tag="ACGI57_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFG9717097.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(11421..11825)
                     /locus_tag="ACGI57_002020"
     CDS             complement(11421..11825)
                     /locus_tag="ACGI57_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFG9717098.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            12847..13761
                     /gene="queG"
                     /locus_tag="ACGI57_002021"
     CDS             12847..13761
                     /gene="queG"
                     /locus_tag="ACGI57_002021"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFG9717099.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIQNISNKEFKR
                     KYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            13867..16104
                     /locus_tag="ACGI57_002022"
     CDS             13867..16104
                     /locus_tag="ACGI57_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9717100.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
BASE COUNT         6448 a         1455 c         3060 g         5215 t
ORIGIN      
        1 aagtagatga tataaatcat aaataaatca aaaattttga aaggaaaata atctatgaag
       61 ctagatttat ttttattagc aataattcct atattgatag ggatgttttg gataaggtct
      121 aaagatagat attgtagaga acccctaatc catctaatta aatttttttt aataggtgct
      181 tttctaagtg ttataattat acttttagaa aatttactga tgaagtttaa tgtatttgaa
      241 gggtattcag aactaatata tgtatcattt gtagttgcag gacttgtaga agaaggtgta
      301 aaagctctga tactgattcc tgccctaatt aaagaaaaac actttactga aaagttagat
      361 ggtattattt actctgtatt tttagcttta ggatttgcaa caattgaaaa tatggtttat
      421 attttttctg aaagtagaaa tctagcactg caagttggta ttaatagggc agttatctct
      481 ataccagctc atgtaatgtt tgcaattact atgggctact atatttctaa gtataaattt
      541 gagggaaata aaaataaaag acgtgagtat ttgtttatgg cggtgttaat tccaatttta
      601 ttacatggag tttttgattt catattgatg atagaatata gatgggctat tatattactt
      661 atagtttatg taataatttt gtggaagata aatcttgata aacttgagaa atatatgaat
      721 cactctaaaa aagtgttttt cggtaatctt aggaaaaaga agaagaaata aaatttaaag
      781 gaaagacata aataattaaa gtataataaa tgacacacaa gtaaagcaga ttgaaggggg
      841 attatagatt tgacagaatt accaaaggaa tttttagaag atattgaaga aatactaaaa
      901 gatgaatatg atgattttat aaaaagttat gaggaaagta agactactgg gcttagaata
      961 aatactttaa aaattgacaa gaaaaaatta tttgatttgg agttgtttga tttgaatcaa
     1021 ataccatggg cagaagaagg tttttattat gatgataaaa ttgacagacc tggtagaaat
     1081 ccactgcatg aggctggagc atactatttg caagagcctt ctgcaatgag tgtaatacca
     1141 aaggcagata ttaaagaggg tgaaaaagtt ctcgatatgt gtgctgctcc tggtggaaaa
     1201 tctacatata tactatctaa gctaaacgat acaggtttat tggtttctaa tgaaattaat
     1261 gctagtagaa ttaaggctct aggggaaaat ttagagcgat ttggagcgag aaattgtatt
     1321 ataacaaaca ccgattctaa tagcttaaga aagacgttta atgggtattt tgataaggtt
     1381 attatagatg caccttgttc tgggcaaggt atgtttagga aggatgaggt tgccatatca
     1441 gattggagtt atgcaaaggt agtagaatgt caatccatcc aaaaagagat tataagagat
     1501 ggatataaaa tgttaaagaa ggatggcatt ttaatttact caacttgtac tttttcaagg
     1561 gaggaaaatg agcatgttat aaatgagttt atcagtgaat atccacatgc agaacttatt
     1621 gaaatggaaa gattatggcc tcataagata aagggagaag gtcattttgt tgccaaaata
     1681 aaaaaattag aagaagaaga atgtaatgtc aaagaattaa aaataaaaaa tttagataaa
     1741 gaaataaagg attataggga gtttgaaaag aaatttttaa aggttgattt ttatagtaat
     1801 aaaaaaaata aattttattt aagaggagaa aatttgtacc tacttcctga aatatatcca
     1861 gatacgaaaa aactaaaagt attgagatat ggtttgcatt taggggtttt aaagaagaat
     1921 agatttgagc cttctcatgc actatctcat tacttaaaag ttgaagatgt aaaaaatgtt
     1981 gaaaacttca gtgtacagag tgaatcaatt cttaaatatt taaaagggga tgttgtaaat
     2041 tctaatgata gtagaggatg ggttttggtt tcggttgaag gaataccttt aggttgggga
     2101 aaagaatctt ctggtgtaat taagaatcat tatcctaaag gtcttagaaa agtattttaa
     2161 actattagaa aaaagaagta taatatttat attgtaggtt tatggtagat tattttgctg
     2221 tagattgact taatatttaa cacagaggag acgattttag tgggtgaagc agttaaaaaa
     2281 gaagttgtag aatggataaa agtgattgtc atagctcttg ttttggcatt tgcaataact
     2341 cgttttatag tgccaacaat agtcaaagga gaatcaatgt atcctacatt agttgaacgt
     2401 gattatttga tagttaacag aattgcgtac aaggtaggag agccaaaata caaagatata
     2461 atagtattca aaaccgactt aacagaggaa aatggaaaga aaaaagattt agtaaaaaga
     2521 gttatcgggg ttcctggtga ccatgtaaaa atacaagact ccaaggtata tgtaaatgat
     2581 aagttgttag atgagacttc ctatatacat aataatcgta ctgatggaga tattgatatc
     2641 gtagttccag aaggaaaatt atttgcaatg ggagataata gagaaaaaag tttagatagt
     2701 agatacgatg aggttggatt ggtcgacgag cataccattt taggaaaggt tctagtcaga
     2761 ttgtatccat tttctaagat aggaactatt gactaaacat ataaaatttg gagcaatcgc
     2821 tgattagctg aaatcaccag accatacaca atagttgtct atataaaaaa gacaacacct
     2881 tatttagtag acttatctaa aagatagtgt ttaagagaca ttatcaataa agataatata
     2941 actataaagg agaatttgtg aatgaatgaa actattaaag aagagattgt agagtggata
     3001 aaaataatta ttactgcact tttttttgca tttattataa ctcgttttat aaaaccaaca
     3061 ttagtaaatg gagaatcaat gtacccaaca cttaaatcac atgattattt ggtagcaaac
     3121 aggatgacat ataagttatc agaaccaaaa tgtggagata taatgatatt taagactgat
     3181 ttattacaag agaatggaag gaaaaaagag cttgtaaaaa gggttatagg tgttcctggt
     3241 gaccatctaa aaattaagga ttctaaggtt tatataaatg gtaagttatt aaatgaagtt
     3301 tcatatatac atgataatta tactgaaggc gatattgata tggtgattcc taagggaaaa
     3361 gtatttgcga tgggagacaa tagagaagtt agtttagaca gtagatataa agaagtggga
     3421 ttagtagatg aagaaaatat taaaggaaaa gttattttaa gagtatttcc ttttacagat
     3481 ataggtattt ttgagtaggt gatttgtatg gcagttggta tagcgtcctt attgtttaat
     3541 attgaggagg ctttaaacat ttgtgagagt ataaagcaaa taactcattt agagattgga
     3601 attgacaata tatctgaatg tagtgagtta tgtaaatata aagagagaat ttctaagtta
     3661 ggattatcta tagggataca tttgcctatg gaattaaata cttgcgaaaa catagagtat
     3721 atacgaaatt catggataaa ttttattgag aagatagaat ttgaactaaa aggatttgat
     3781 ttaaggtatt ttaaccttca tttgggatat gttatgacaa atagagttag aaagaataga
     3841 gacaaatatt taggaaatag tgtggatttt ttagataaat taaatacaaa ttcatatgta
     3901 tgtatagaga atacttattc aaaaggtggg gatttttcaa atattggaaa tatatcttat
     3961 gattttgaat atatatttaa gaggattaaa aattctaaga tttgtttttg ttacgatact
     4021 ggacactgtt taattgatga ggatgcttat gtaaagaact taaaggataa aataagactg
     4081 atacacttaa gtgataatga tggtatcaat gatacacatg ttggtatagg aagaggaatt
     4141 ttatctgaag aggggattaa agagatctta acattagatg cagaatattt agtattagaa
     4201 ataaattacg aagacattga agatactata agtaaattga ataatattgt aggagagggt
     4261 taaatacctc tctttatctt gttgatatgc ctcattaatt aaaatttata ttcatcaaaa
     4321 ggaaggagat gttgaagtga aaaaaataaa cttaaaaata gaaggaaaag ataaggaata
     4381 ttcattagaa gaaaattccc ctggaatcag attgggagat atagcaaaag aattttgtga
     4441 tgagcataag ggttatataa cacttgcagt tgtagacaat aaactaaaag agctaaattg
     4501 tagagttaag aaggattgtg agataaattt tttagatact actaatgaag atggagagag
     4561 agtttacttt agagtcatgt catttatatt tgtaatggca tgtagagaaa tcttttggga
     4621 tagcagagtt acaatagaac attcactatc agatgggcta tattgtgaag ttcatataga
     4681 tagaaagctt aaagaagcag atgttgaaaa tataaaaaac aagatgaaag agatagtaaa
     4741 caacgattat gttatagaaa aaattgaagt tacaagaaat gaagctattt ctatctttga
     4801 aaataatgaa atgtatgaaa aggcagagct tttgaaatac aaagagtatg atagtgctaa
     4861 agtatataag tgtagaaact atatagacca tttttatggt tatatgttgc catcaactgg
     4921 ttatataaaa tcatttgata ttaagcttta taatggaggc gttataattt taggacctag
     4981 tgaagaagat aagactcttc ctatgaaatt tgtacctcaa cctaagctat caagtgtata
     5041 ttatgaagca gaagaatggt caagacttat gggaattgac aaggtagtat ctcttaataa
     5101 aataatagaa aacaatgaat atggagatat aataaggaca tttgaagcat tgcatgaaaa
     5161 gaagttatcc caaatagcgg atatgataaa ggatggcaat aagagagtag ttttaatagc
     5221 agctccatct tcatctggaa aaacaagttt tgctcacaga ttatcaatac atcttagagt
     5281 aaataattta aatcctattt caatttcttt agatgattat tttatagata gaaaacatac
     5341 tcctttagat gaatatggaa attatgattt tgaatcaatt tatgcaatag atttagagaa
     5401 gtttaactta gacttaaaga aacttttagc tggtgaagaa attgatgata taagattcaa
     5461 ttttaaagaa ggtaagagag aatacactgg taaaaagata aagatagatt caaatcagcc
     5521 aattatatta gaaggtatac atgggctaaa tcctatttta acatcatcta taccagatga
     5581 agataaattt aagatataca taagtgccct tactcaaata aatttggatg accataatcg
     5641 aatccctact gctgatttga gaatgataag aagaattgtt agagactata attttagagg
     5701 atatagtgct gataatacta ttttacaatg ggcatcagtt agaagaggag aaaagaagaa
     5761 tatattcccg taccaagagg aggcagatgc tatatttaac tctgcttgtg tatatgaatt
     5821 ggcagtttta aaacgatttg cgaaaccttt gttggaagag ataaaggaag ataatcctgc
     5881 acatattgaa gctactagat tactaaagtt tttgcagtat tttgttgaat tggacgatac
     5941 ttctgacata ccaggtattt caattcttag ggaatttatt ggaggaagta aaatagttga
     6001 ttaatgtgtg tattatagat tgttttatga agattaaatg gcatagcaag tagtgttatt
     6061 ggtttatgtt aagtatatta agtgtaaagt ggagaattaa attgatacag attttaaagt
     6121 ttgagttgat atataaaaag gcttgtttta tacttttgta tgaagcaagc tttttatttt
     6181 aaattttact tttttgttta atattttgtt aatttatgtg attatttttt aaaagagtaa
     6241 aagagtaaaa gagtaaaaga gtaaaagagt aaaagagtaa aagagtaaaa gagtaaaaga
     6301 gtaaaaggtg ttgggttaga aatttttggt gttataaaaa tttaaattac tggattatta
     6361 gattgctgga ttgctaaatt actaggttgt taaattattg aattattagg ttgttgaatt
     6421 actaaattac taggttgtta agttactgga ttatttagat agttagatta ctaggttgtt
     6481 aaattattga attattaggt tgttgaatta ctaaattact aggttgttaa gttactggat
     6541 tatttagata gttagattac taggttgtta aattattgaa ttattagatt gttgaattac
     6601 taaattacta ggttgttaag ttactggatt attagatagt tagattacta ggttgttaaa
     6661 ttgttaaatt attgaattgt tagattgttg aattactaaa ttactaggtt gttaagttac
     6721 tggattatta gatagttaga ttattagatt gctaaattat taggttgtta aattactgga
     6781 ttattagatt actaaattgc taggttgtta aattattaaa ttattaagct atttaaacta
     6841 tttaaattgt ttgaaaaatg aattgattct tgtagaaaat aattttaaag tataattttt
     6901 tttaacattg ttttattata gtataaactt gtgctaaaat agaatatata acataatata
     6961 agaggtgcat atgaatacgt tagatgagac attattaaaa gaaattataa gttctaataa
     7021 gaagtatact aattatggac aagtcgctag ttacatacca gaattaaaaa acgctagaag
     7081 aaatgattta ggaatttgta tcatagatag cgaaaataat ctttatagtg ctggaaactg
     7141 tagcacaaag tttactatac aaagtatttc aaaaccaatt gtgcttgcta tggcacttat
     7201 ggataatgat tgggaagacg ttttttcaaa cgttggaatg gagccaagtg gggacccatt
     7261 taattcaata atgaaacttg aaattaatga cacaaaaaaa ccctgtaatc caatgataaa
     7321 tgcaggggct atagtaacca cttcccttat aaatgggagt tgtttagaag aaaaagaaga
     7381 aagaatgctt agtttcttta gaaagttagc aaaaaatgat aatataggaa taaactatga
     7441 tgtctataag tctgaaaaaa tgactggaga tagaaacaga gctatggctt acttattgaa
     7501 aagtgatgga tttatcagag ggaatgtaga agatgttcta gacctttatt ttaaacaatg
     7561 ctcaattgag atagattctg tagatttagc gagaattgga ataaatcttg caaattatgg
     7621 agtagatata gaaaatggtg agcatttaat gagtgaaatg gtttctagga ttgtcaagac
     7681 atttatgatg acttgtggaa tgtatgatgc ttctggggaa tttgctatta aagttggcat
     7741 tcctgcaaaa tctggagttg ggggtggtat aatggcttct gtgccgggac gcatggggat
     7801 tggtgtttat ggacctgcgc ttgacaaaaa aggtaacagc gtcgcaggag ttaaagtttt
     7861 agaggagttg tcaaataaac tcaaattaaa tattttttaa aatattaaga aaggattttt
     7921 ttgacaacaa catataattt aatatataat taaattgtaa agaaatatga attaaataaa
     7981 ttttttagga ggggtcaatg aaagaggtat tggtattacg agatttggag tgcataaagg
     8041 ctattgcgca tccacgtaga atagatattc taaaagcatt tgacaaatcg ccattgtcag
     8101 caaaacaatt atctcaatta ttggaagaac ctcatgcaaa aataaactac catataaaaa
     8161 tgttatacaa agttggtatt ttagaattag ttgaggaaaa aataaagtcg gggattgtgg
     8221 aaaagtacta ttatccaagt gcaaaaaatg tggttatagg gaataggata cttaattttt
     8281 ctttagacaa tggggaagaa aaagaagaat tgtacatatc aaaatttgaa aatatgagtg
     8341 aagtttttta taaggcagca gaggaagatg ttttagaaaa tgaaaatata gttgattatc
     8401 atgatatttc tcttactcat gatgaattag ttgaacttag tgacactatg aagtcaaaga
     8461 tagatgaaat tttaaataaa agacaacaca atgttgaagg ttctaagtat gatatagcta
     8521 tggttatagt tcctactctt gaggaggaat gtcctagtta gagaattaat tatggattaa
     8581 ttaaagatat aatattagag attgaacatc aaaatttact taataatcaa aaaatagttt
     8641 ttggtatggg taaaacaatg atttttaatc tctatttttt tgttttttta tacctattgt
     8701 atttgtaaat aatatgtata gtgtaagatg tatacactat ctaagaagtg cttagataat
     8761 atacaaagta tgtaagatta taatatttga aattgattaa aatgggtata attaaagtgt
     8821 aaatattaaa attaaaaatt aatataaatt aaatcaatgc tagaaatatg ttaaaagagg
     8881 aatgagatta gttgggatag aacatccctt agagcttacg aatttttagt gaaaaagtac
     8941 tatcaaatag gaatctccat cttcatatgt tggctaaatg tagttaaggt ggagtagttc
     9001 aaaattaaaa ttgagtacaa gtagtagtac aaataaaagg agattaatat gattaaatta
     9061 ggaacacatc tttcaatagc aaaaggattt gctaatgcgg ctgagacagc tgtatatatt
     9121 ggtgcaaata catttcaatt ttttagtaga aatccaagag gaggaaatgc taaagaattt
     9181 gatgaaaaag atataattaa atttcaagaa attagaaaag aaaataattt tgcaccactg
     9241 cttgcacatg caccatatac tatgaatctg ggtggtacaa aggatgatgt atacgaattt
     9301 gcaacaaggg tgatagatga ggatataaga aggatggact cattaggaat tgaatacatg
     9361 tgttttcatc ctggaagtca tgtaggtggt ggtgtagatt ttggcataga tagaatagta
     9421 aaaggattga acaatgccat aaagggtgat gaaaatataa ctatacttct tgaaactatg
     9481 tcgggaaagg gaactgaaat aggttttaaa tttgagcaat taaaaagtat aatagacaaa
     9541 attgagcata aagaaaaaat aggtgtgtgt cttgatacat gtcatatttt ttcagctggt
     9601 tatgatatag tgaatgattt agatggggtt ttggaagaat ttgataaggt tataggcata
     9661 gaaagattga aaactgtcca tctaaatgat agtatgatgc catttggaga caaaaaagac
     9721 agacatgctc ctatagggga aggaaagata ggattaaaag cattaattga ttttatggaa
     9781 catcctagtc taaaacattt accatttttc ttagaaactc cttttgatga tgaaggtcac
     9841 aagagagagc ttaaaatgat aaaagaaata ctatctagta aataaacctt tattaaaaat
     9901 atgtgaaata ctccaccctc aacataatta gggtggagat acaatcatga aatgtgataa
     9961 tttaaattaa tggataggaa taacgaaaat tgtatacaaa ttaactgggt ataaagtatg
    10021 attatattat gaatagactg atataaaata taattatatt atgaatagta tgtaaataca
    10081 ttgcaaagat tctcataatt tgttgtataa ttaaaggtga catatttata ttatataaaa
    10141 aattattaaa agttaggtgg ggaaagatat gagaaattta ggcaatacta acatgaaaat
    10201 aaagagagtt ggctttgggg gcattccaat acaaaggatt actcaagatg atactaactt
    10261 agttataaat gagttagaaa aacaaggaat aaactttatt gacagtgcaa gagggtatac
    10321 aataagtgag gaagcaatcg gaattgccat tgaaggaaaa agagataaat ttttcttagc
    10381 tactaaaagt atgtccagag attatgattc aatgaagaga gatgttgaaa taagtttaaa
    10441 taattttaaa actgatttta tagatttata tcaattccat aatgttaaag aagaggagta
    10501 tgataatctt tttaaagata aaatggctta tagtgccctt ttagaggcaa aggagcaagg
    10561 taagataaaa catattggta taactagtca caatttaaat actatagaaa aagctataga
    10621 agatggaaaa tttgacacta tacaattccc ttataatata gttgaaggac aagcagatga
    10681 agtgtttaaa aaagctcatg aaaaaggaat tgggataata gtaatgaaac ctttagctgg
    10741 aggtgcttta gacaatgcaa cactggctat taaatatata ctatctaaag agtacataga
    10801 tgtagttata ccaggtatgg agagtgttga acaggttaga caaaatgtcg ctgtacttga
    10861 aaatcttgtg ctagatgaaa aagataataa ggaaattgaa gaaataagaa attctttagg
    10921 taaaaaattc tgtagaagat gcgaatattg tatgccttgt gctgtaggaa taaatatacc
    10981 attgagtttt ttatgtgaag gttattatac tagatatgga ttaaaagagt gggctaaaga
    11041 aaaatatgag gttatggatg ttaaacctac tgagtgtatt gactgtgggc tttgcgagag
    11101 tagatgtcca tatgaacttc ctataagaga aatgcttaag actgttgttg aaaagctagg
    11161 atagatttta tttattgaaa tcggtgtatt aaatttactc cgatttcttt ttattagatg
    11221 tggtcttata tacaaatatt aaatttacat agttatagct attatataaa tttaatattt
    11281 gtttaatact tattaaatat ttacttaatg ctttttaaaa acatttgttt gagtatagac
    11341 ataacacaat tattattgtt gtattagtat agtttcaaat actggagtat caatataatg
    11401 tcgaataaca cataaaatct ttatttttca aaaatattat aggatatatc gtatttagat
    11461 actggataaa gttcaatttc aaccctaggg ttatctttat ctataaaatc aaataaaagt
    11521 actggcttaa gttgtttatc attttttatt attccacttt tttcaatacc atcacaaata
    11581 ctttttggat aattatgtaa atcccccatt ctcttatttg ggaagaataa tttcaagact
    11641 gttattagat tttcttcgac agtttcccct tgatactgtt ggtttataaa acctgcaatc
    11701 atgttttcgt atgcaagata cttagaatat tttccctttg aaggcatcca tgcttgacca
    11761 tttatgttat gaagcttaaa attagattta ctaataggtc tacctggaat tgttatttta
    11821 atcaaatttt tcaccatcct tatacagtat ttattataat ctgtatatga tttaaaagca
    11881 aattgttatt taaagtgttt ttacagagct tttttgtaaa attttccact ttagatgtag
    11941 taatataaga taagtatttt gcttacaaat tttaaaattt gatatatgat ggacatattc
    12001 atattagtct attataagac cttcataata ttaataagta tagtttagta aagagagtaa
    12061 aaagaaatcc atataagtca agtaaagatt taataaatgg caatgtaaat gggatattaa
    12121 ttataagaaa aaatcaatct ttttgcttaa ctatagaaag aatgtcaaaa tataactaat
    12181 gatatttgaa gttttagagt ttatattaaa aggtaagaag gttaagtggg taaaaaaggt
    12241 tgaatagatt aaaaaggcta agtatctaaa agagttaagt aggtaaaaaa gttaaataag
    12301 taaatgaaaa aggctaagta tctaaaagag ttagtagtta aataagtaaa tgaaaaaggc
    12361 taagtatata aaggagttag tagataaaaa aattaaatag ataaaaagat taagtatcta
    12421 aaagcgttag tagataaaaa aattaaataa ataaaaaaga ctaagtattc aaaagagtta
    12481 gtaggtaaaa taattaaata gataaaaaga ctaattattt aaaggagtta agtagataaa
    12541 aaagttaaat aagtaaatga aaaagacgaa gtatctaaaa gagttagtaa ataaaaaaga
    12601 tgaagtatct aaaagagtta gtaggtaaaa aactaaatag ataaaaagat taagtatcta
    12661 aaagcgttag taggtaaaaa attaaatagg taaaaagacg aagtatctaa aagatttaag
    12721 taggtaagaa aattgaatag ctaaaaagat taagtattta aaagagttag tagctagaaa
    12781 aggttaaaaa gactaaaaaa attaaataaa tttaaaaagt caattagatt aaaaaggaat
    12841 aattttatgg ataataagga attaaaagca ttttgtaatt taataggatt aaactgtgta
    12901 ggagtagcag gggtagataa gtatgataat ttagaaaaga tacttaagga cagacaacaa
    12961 aaaggttatt taactggtat ggaagaacca ataattgaga acagaattaa cccaagacat
    13021 ataatgaagg atgctaactc tataatagtc tgtgcatttc catatcatat aaatagacaa
    13081 gaaaagaata aaaattcaaa tttatcaaaa tattgtcatg gaaaggatta tcacattgta
    13141 gtaaaggact ttttacaaca aatttgtgat tatatttcta aaacagtaac agattttaaa
    13201 tacaagttat ttgctgataa tggaccatta gttgacaggt atttagctta tttatcagga
    13261 ataggatatt ttggaataaa taataatatc ataactgatg aatatggttc ttacgtattt
    13321 atagggtata tagtaaataa ttataaattt gaagaagata aaccactaga aaaaagttgt
    13381 tttaaatgtg gaaagtgtgt aaaatattgt ccgggaaatg ctatattggg aaattatgat
    13441 atgaacccca aaaaatgttt atcatatata actcaaaaaa aaggagattt gtcagagaaa
    13501 gaaaaagttg ttttaaagaa tggcaaaaag gtatttggat gcgatatttg tcaagaagta
    13561 tgtccacata atgttgaaat accaacaact catattttgg aatttaaaga aaatataata
    13621 gattatttga attatgatga gatacaaaat atttcaaata aagaatttaa aaggaaatat
    13681 ggagatagag cttttagctg gagaggaaga aatataataa agagaaatat ggaaataatt
    13741 ttagataagt atgatgacta ataataagag tctgtttcat atactttaat gtgtatttac
    13801 tttaacactt gttgtgtaaa agtcatatta tatcattaaa gttaaatatg gggaggtggg
    13861 ggaatattga aatataataa tacatctgaa ataaatccac tagattatat attgggtcaa
    13921 gaaagagctg tagaagctat ggaattaggg ttgaaaataa ataatccagc gtacaacata
    13981 tatatagctg gtgaacctgg aactggtaaa agtacttatg ccttaaaggt tttaaatgag
    14041 tatgcatcca aaaaaaatac gcataaagat tggtgttata tatataattt tgaaaaccct
    14101 agagagccta ttatagttga attagaaaag gggtttggaa gagaacttaa gaaagatatg
    14161 gagaaattaa ttgaaagtct tttagaagat tttaaaaatg cgtttgaaag tgaaaatttt
    14221 gaaatagaaa aaaataaact tctggatgaa tatgaaatag aaaaagatat gcttttgaag
    14281 caaattaaaa aatatggaga agaaaaagga tttaaattaa aacagagtaa aatgggaata
    14341 gtttttatcc ctttgaaaga agaggaagat gaaagtgatg agtctgatga agagttttac
    14401 aaagctaaaa gagaactaga aaatatggcg atacaggtcg tatataaaat tagaaactta
    14461 gaagaaatag ctgaaaaagc agtgttagag cttgaagaag agatagctaa acttgtagta
    14521 gaacctcata tcaagagatt gtgtgaaaaa tatgaaaatt atgataagat tcaaacttat
    14581 ctggaaaata taaagaaaga tataatagaa tatatgtatt tattctattt agatgaagag
    14641 gagttaaaag ataagtatga taaagagcat tttattaaat ataaaattaa cctatttgta
    14701 gacaatggaa gctcaaagaa taaagaatca gcacctgttg tagtagagat aaacccaagt
    14761 ccagcaaatc tatttggaaa ggcagagtat gattatgcaa atggaaatat aaagactgat
    14821 ttcacaaaac tattatcagg agcatttcat agggctaatg gtggttattt agtactttat
    14881 gcagaccaat tactaaaata tacaatgtct tgggaaattc ttaaaaagac tttgcagact
    14941 aaaaaagtaa tattggaaac acaaacagca attaaaccag aaaatatgcc attggatgtt
    15001 aaattagtac tgattggaag ccactatata tatgatattt tatatagata tgatgaagat
    15061 tttgaaaaat attttaaagt atttgtagac tttgatagtg aaatggataa aaatgaagat
    15121 aatgaagaag gaatagcaag atttatagca taccaatgtg ataagaataa tttaaggcat
    15181 tttactaaat cagcagttga agaggttatt aaatttagca ctagattatc tggtgattta
    15241 gaaaaattat caactaagtt taataaaata atggaagttg taatagaagg aagtgcttat
    15301 gcagagttta gaaattcgga gtatacagag cagtgtgatg ttaaaaaagc aataagtgaa
    15361 aagagaaaaa gaatcaatag agtggaaaca catatggatg aatcaattga aaatggattt
    15421 actctaatag aaacagaggg aagaaaagta ggagtgataa atggtttgtc agtcttaagt
    15481 acaggagaat actcttttgg tagagcttca aggattacag cgaccactag cccaggaagt
    15541 aaaggcatag taaatataga aagagaagta aatatgagtg gctctataca taacaaagga
    15601 gtacttatat taggtggcta tctttctgag aattttgcac aggatttact attgtcacta
    15661 aatgcatatg tttgttttga acaaaattat ggtggaatag atggagatag tgcatcttct
    15721 gcagaattat atgtactatt atcatctctt agtggagtat caattaaaca aaatattgct
    15781 gttacaggtt ctataaatca aaagggtgat atacaagtag ttggtggtat aagtgaaaag
    15841 atagaaggat tttattctat atgtaaaact aaagggttaa atggtgagca aggcgtcata
    15901 attcctagaa aaaatcaaag aaatctcgtt ttatcagatg aagttaatga tgcagtgcga
    15961 gatggaaaat ttaagatata tactgttgaa agagtagaag aggctataga gatattgaca
    16021 gatgttaaat ttgaagaaat aaagaaattg gtaaaagaaa aattaatatc tttcagcaag
    16081 atacaaactg tgtcaaaaga ataagtttat tatagaattt aatgggtatt tatgtataat
    16141 aaaaagcaag aactttaata ggtttttgct ttttttgt
//
LOCUS       DAYAOX010000084        16008 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.084, whole genome shotgun sequence.
ACCESSION   DAYAOX010000084 DAYAOX010000000
VERSION     DAYAOX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.084"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(128..1318)
                     /locus_tag="ACGI57_002023"
     CDS             complement(128..1318)
                     /locus_tag="ACGI57_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFG9717101.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(1578..2009)
                     /gene="dut"
                     /locus_tag="ACGI57_002024"
     CDS             complement(1578..2009)
                     /gene="dut"
                     /locus_tag="ACGI57_002024"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFG9717102.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            2122..4350
                     /locus_tag="ACGI57_002025"
     CDS             2122..4350
                     /locus_tag="ACGI57_002025"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFG9717103.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(4461..4946)
                     /locus_tag="ACGI57_002026"
     CDS             complement(4461..4946)
                     /locus_tag="ACGI57_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9717104.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            5198..6421
                     /locus_tag="ACGI57_002027"
     CDS             5198..6421
                     /locus_tag="ACGI57_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFG9717105.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPSILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            6425..9559
                     /locus_tag="ACGI57_002028"
     CDS             6425..9559
                     /locus_tag="ACGI57_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFG9717106.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            9581..10891
                     /locus_tag="ACGI57_002029"
     CDS             9581..10891
                     /locus_tag="ACGI57_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFG9717107.1"
                     /translation="MNKKMSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSD
                     SNKRTSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFP
                     YDQIGLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMK
                     LKKDLGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEY
                     RFESFRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVA
                     PTKPEQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFN
                     TENGLVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGL
                     ITLTDYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(11180..11425)
                     /locus_tag="ACGI57_002030"
     CDS             complement(11180..11425)
                     /locus_tag="ACGI57_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFG9717108.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(11639..14266)
                     /gene="ppdK"
                     /locus_tag="ACGI57_002031"
     CDS             complement(11639..14266)
                     /gene="ppdK"
                     /locus_tag="ACGI57_002031"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HFG9717109.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(14327..15169)
                     /locus_tag="ACGI57_002032"
     CDS             complement(14327..15169)
                     /locus_tag="ACGI57_002032"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFG9717110.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(15176..15814)
                     /locus_tag="ACGI57_002033"
     CDS             complement(15176..15814)
                     /locus_tag="ACGI57_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9717111.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
BASE COUNT         5612 a         2541 c         2300 g         5555 t
ORIGIN      
        1 aataaaaagc aggtatcaat caaatagtaa catgtattat actatacatt actatcaata
       61 ttgtacctgc tttcatatta taagcaataa agaaaaatct atttattagg ttttataata
      121 aaaacaatta taaaactctt cttgcattta catatctact tgaatagtag ctactattta
      181 tattagatat tgttactttt tttgatgaac ctgaagatgc atgtatcata tctccatttc
      241 ctatgtatat tccaacatga cttacagaac cattatttga accttgtgta tcaaagaata
      301 ctaaatctcc acttctaagg tctcctctat ttacatatgt tccatactta ctttggtctc
      361 tagatactct tggtatacta actcctacag attttttcat aacgtactgt gtgaatccag
      421 aacagtcaaa actatttgga ccttctgctc cccatacata tggttttcca agtagtgtct
      481 ttgcaaaact tattacacta tcagctcctt gacttggaga attagtcgaa taagatggaa
      541 ttgaagtatc aacagaacct ttttcagcaa ggtattgact tgacacatat ccagtttgat
      601 tgccataact tacttttgtc catcctccac tttcagaaat acttccaacc tcagtaccat
      661 aacttaatgt agctatttta gaatgacttg tacctggtcc tgttctaacg ttcaatgact
      721 ttgctgttac aacctttgta ccctttacaa ggtcatttga actgttttca gcattttcat
      781 ttgtagagtc atttacacta gttcctaaat atttacttga aacataacct actctaccat
      841 tagaacttat ttttgaccat ccatttgact cagatatgta tccgacttca gtcccatatc
      901 ctagtgttga tattttagaa ctacttgtac ttggtcctgt tctaaaattt aatccttttg
      961 ctgttacaac ttttgtcgac ttaactgaag tatcactact ttcattgcta cttccaaggc
     1021 tatttattgc tacataattt ccatgcacat aacctgtgtt tccattatat ttaactttta
     1081 cccaagaacc ttcttttcct acgtactcaa ctttatcacc tttgtaaagt ttccccattg
     1141 attcatggtt tgtacctggt ccttttctaa aatttaccgc attccctgtt attgttctgc
     1201 tttctatact cacactctct acttcagact cattagcata tgctaaattc cccatagtag
     1261 gcattattat cgcagttgcc attatactgg ctgctattct cttttgatta aaattcataa
     1321 ctttccactc atctcctctt ttctactatc tttatttcac ttcaaaatat aaaagaaata
     1381 attgacacta ttaaaatatc aattatttct tttatattaa tattattaca aatttgtaac
     1441 tatttgtcta tagtaatatt tgcttaccat aaattaccat gtataaattt taatctttaa
     1501 tagcctgtaa tttagtaata tagccatctt ttaccttttt taaaaatggc tatatttttt
     1561 aacttttttg taacttttta aaatccagtt gagccaaacc caccttttcc tctttcggag
     1621 tcacttaatt cttttacttc ttctatgtta atatcataga taggttttac tatcatttga
     1681 gcaattttca tatgcttttc aaccttaaaa tcatttttac catgatttat taatataatt
     1741 ttaagctctc ctctgtatcc ttcgtcaata gttcctggtg tatttaatac agtaacagaa
     1801 tgctttaagg caagcccact tctaggtctt acctgcgctt ctgtcatagt aggaagctct
     1861 atacatatcc cagttttgat aagtttagtc tctccaggag gtattacaac ctcttctata
     1921 gaatacaaat ccataccagc atcacctttg tgagcaaaat taggtattat agcatcatca
     1981 tttaattttt ttacttttaa attgtacatc ttatatctcc tttcaacttt ctataaatat
     2041 tataacttaa atttcgacct cttgaagttt tttatttttt tgtggtaata taattattag
     2101 tgttttaaag gaggattttt tatgaaactt gtacttgctg agaaaccatc tgtggcaaaa
     2161 actatagcat catttttagg tgcaaagacc agacaagatg gttactttga aggaaatgat
     2221 tatataatta cttatgcagt tggacactta gtatcattat atgatatgaa ggattatgat
     2281 aaagataaat actctggctc ttggaaaatg aacaatttcc catttgttcc tgaggataag
     2341 tttaaattta agattgatag ttctaaaact aaacaattca acactgtaaa aaagcttttg
     2401 aatagaaaag atgtagaata tgtaataaat gccactgaca atgaccgaga aggagaactt
     2461 atagcatttt taatattttt actggcaaaa aataaaaaac cagtaaaaag aatcttggta
     2521 aatgaatgga caccagaaga tattacaaga ggtataaaaa acctaaaaga tgaagatgaa
     2581 atgagaaatc ttcaagctgc tggatatact aggcttatta ctgactggtt gattggaata
     2641 aatttcactt ctgttgctac attgaaatat ggaaatggga aactattaaa cataggtagg
     2701 gtaattcttc caacagttaa attagtctat gatagagata tggaaatact aaactttgtt
     2761 cctaaaacct attatgaaat agaagggcat tttaaagctg aagctggtga atataaaggt
     2821 aaatatgtta agggtaaaga aagtaaattt gatacacttg aagatgcaaa taaaattata
     2881 gcttcaatta catctgaaac tggtaaaata ctagataaaa aagttactat gtcaaaggaa
     2941 tatgcaccaa aactttttag tttgacatca cttcaaggat atataacttc taagtattcc
     3001 aattttacat ctgataaggt tttaaatgtc tgtcaatctc tctatgaggg taatggtaag
     3061 ggtggataca taacttaccc tagaactgat tccattttct tagaagaaag tttggcttca
     3121 aaagcttctc aaactttaga taaattaaag gttggtcttg aatatgaaaa taaaattaaa
     3181 ttttctaaaa ctaaaagagt ttttgattct tctaaagtag actcccatag tgctataatt
     3241 ccaacttata taattccaaa aagtttatct aaggatgaac aattagttta tgatgccata
     3301 aaagatagat ttgtagcaaa ctttatgcct cctgctgaat atgagaatac agaaattaag
     3361 actgaagtag ataattgtac tttcttaaca aaaggtaagg tgcttaaatc aaaaggatac
     3421 cttgaggtat ataataagga agaaaaaaac gatttacttc cacttgtaaa taaagatgat
     3481 gttgtagatg tgttagaaat aaaaccatta actaagcaaa ctactccacc taaaccatat
     3541 actgaggaca cacttttaaa ggctatgaaa aattgtggta aaaatgtgcc tgaagaagat
     3601 acaacagtgc tatctggata ttcgattgga acatcagcca cacgtgctga tgtgttaaag
     3661 aagataagtc aagtgggcta tgtaaagaaa aaaggtaaat cttattctat aacagacctt
     3721 gggaaaaatt tagttgaaat ttttcctgtt aaagacttat ttgatgtaga ttatactgga
     3781 aaacttgaaa aaagtttaag cgatattcaa aaaggaaagt atacaagaaa agaatatctt
     3841 actaatatca ttagttttat atatcaaaat gtcaatatta taaaacaaga tgcttcaaaa
     3901 aatataaaca cagaagcata tactttcgat tcaaaaacta aaaaatttac taaaacaaaa
     3961 gaaaaaaaac agaaaaaaga tgtaactaat actgaaaaag aagttgctat atcaaaggat
     4021 tctaataaca aaaaagaagt taaaaatggt gatgaaaatc aatctcttgg caaatgcccc
     4081 atttgtcaag gtgatgtact agaatttgac aaaggctttg cttgtaaaaa tcataaagaa
     4141 tgtaaatttg ttatttggaa aaatgataag tatttaagtc tttataaaaa gaaagttaac
     4201 aaaacaatgg tcaaaaatat tttaaaaaaa ggtgaaacta aagtaaaatc tttaactgct
     4261 aaaaacggta ataaatttga tgctacatta aaatatgata aaaatccaga tactggctat
     4321 ttcaattgga aaatagagtt tgacaactaa atattcataa gaaaagaaat agcagtagta
     4381 taataatcta tattatatta ctgctatttc ttttcttatg aagttttaac ttttttaata
     4441 gtgactttta caatcacact ctaacctaat ttctttacta tttctgataa ctttaatgta
     4501 tgttcatcta ataaagttag ttcctcttca gaaagattag ataatttttt tactagagat
     4561 tctttgagaa tagcttttac tgcatcaaat aatttaaatg ctttatctgt taattcaacc
     4621 ctaagaatcc ttctatcttt tgggtcagga aatttattga ctaaaccatg ttccactaat
     4681 ctgtcaataa tcggagtcat atttgatttt aaaattccta aattagaagc tatttgagaa
     4741 attgatgatg aatttgatgt cataagataa agaattacct tagcatgtga tggagccatt
     4801 ggacattctt caaaataagc tttcattata tcagagttat tttgaaactt ttttaacaaa
     4861 tcgctttctt taaatatatt attttcaagc acaaataaaa aatctaaaaa agagtccgct
     4921 attttaagta tattgttgtt atccatattt gtaacatccc ttcaatagat aatataataa
     4981 aacattgttg tttaatatag tataaattat aaactattaa taaatttaaa acaagaaaaa
     5041 gttattgact cataataatt ataaatttat aatcaaatta gagaattata aattgccaat
     5101 aaagggaagt tttttaatta gtctaaaact aaacttaaat tataagacac tttaaattct
     5161 acaacaagct agtgtgcaat atataatagg aggagatatg aaaagatatt taatagtttt
     5221 cttaatgtgt agttcactgt ttttagttgg atgtggaaaa acagaagaat ctgaacctga
     5281 gaaaccaata gcagtttctg ttcaaaaagc aataggtgga gaaattgaaa acacaaattc
     5341 tttctcagga actacaaagg ttaaagatga aacagctgtt actgcacaga ctgttggcac
     5401 agttcaagaa gtgtatgtaa aacttgggca aaatgtaaga aaaggagatg aattattgag
     5461 tataagcagt ccagagctag aaaatagtgt aaagcaaagt aaagcctcac tagatttagc
     5521 aaaagcaagt tattcaagtg ccactggtgg ttcattagag gcacaagtaa atcaggcaaa
     5581 aacagctctt gacaatgcaa agattcaata tgaggagtct caaagaaatt atgacaataa
     5641 taagattctt tatgaacagg aagtcattag tttagaccaa tttaaaaaaa tagaattttc
     5701 tttagagcaa acaaaacaac agttagattc tgctcaaaga gcatacgata cagcaacttc
     5761 aaaaagtata cctcaagcta aagcacttgc taaaaaacag cttgaccaag ctcaagtatc
     5821 atataattta gcaatgagta atttagacaa attaacatta acatctccag tagatggtac
     5881 aataacagct aaaaactttg attcaaagga aatgataaca caatcacaac cagcttttat
     5941 tatttctaat cctagtatac tagaagttga tttaaatgta gcagaatcag atataggtaa
     6001 atttaagaaa gatggaaatg tagatgttat aattgaagac caaaggattt taggaaaaat
     6061 agattatgta ccttcagtag ttgaccctca gacatcttta tatcctgtta aagtacttgt
     6121 aaataataca aacaacaaat ttaaggcagg tatgtctgct caagtaaact taagtgttga
     6181 aaaagaaaat ggtgctgtta cagtacctaa aaaagcaata tttgaagaaa atggtaaaaa
     6241 atatgtatac atagcaacaa aagacaatat agcaaagaaa cacttggttc aaactggaat
     6301 tgtaacagag gataaaatag aaattaaaag tggtgtaagt gataaagata cagtgattat
     6361 tggagggata agtcttattt ctgacggaac taagatattt cctgtagaaa aggagaagta
     6421 gactatgaat ttaacacaga cttccgttaa acgtcctctc acaatcataa tggtcttttt
     6481 agtattaata gtttttggtg gtattggata taaaaaaatg tcaataaatc taatgcctga
     6541 tatagaaata ccagttgtaa tggtcatgac tacttggaca ggtgctggtc ctcaagacgt
     6601 tgatgaacaa gttagtcaaa aagttgatga aagtttatct gccgtttcaa atgtaaaatc
     6661 tactatatca agttcacaag aaagtgtttc tatggtagtt gctcagtttg aatttggaac
     6721 taatttggat gaaataatga atgatgttag gtctaaagtt gatgcattac agaccagcct
     6781 cccagatgat gcagctaaac caacagtttt aaagctggat atgaatgcac aggccatagg
     6841 tcaacttgtc atatctggag gaaatgaaaa tagttcacaa gccttaagaa aatacgctga
     6901 agatgtaatt cagcctaaaa tagaatccat agatggtgtt acttcagcag accttaaagg
     6961 tggaaaaaaa gcacaggtta atgtaatagc tgacccagct gttctttcaa attatggggt
     7021 tagtttatcc actataaaag gtgtcttatc ctctagtaat aaaacattcc cttatggttc
     7081 aattactcaa ggtgaggata aaatagtttt aagagcgatt gataaattag aatccctaga
     7141 tgatataaaa cagattcaaa ttcctgtaaa aggaggaaat acagttagat tagataatct
     7201 ttgtagtgta gattatggat atgcagacaa agatagtatt tatagataca atggaaaaga
     7261 cagtttggta atagatattt ctaaacaaca agatgccaac actgtaaaag ttatgcagag
     7321 tgcatacaaa tacgtagatg aattgaataa ggaaaatcct caatttaaaa ttaaaattgc
     7381 taatgataca agttcataca taagtgaatc aataaacagt gtaatgagca acttgcttat
     7441 aagtgctgtt atcgcattta ttgtaatatt tgctttccta aaaagtgtaa gagcttcact
     7501 agttgttgca gttgcaatac caatttctat aataggtgct atagcaattc tctactttac
     7561 tggtgaaagt ttaaatctta ttactgcaag ttcattggta atttcagttg gtatggttgt
     7621 tgataatgct acagttgtaa ttgaaaatat atttaaatat aggaaaagtg gcagattgag
     7681 tcttgatgat tgtgctattg agggaacaag aactgtaaca aatgcaatac ttgcatcaac
     7741 attgaccaca atagcaatat tcttacctat catgtttaca gaaggtatag ctaaaataac
     7801 ttttggggca ttaggtaaaa cactaatagc ttcactagca ttctccttta taggtgcaat
     7861 tacactcgtt cctagtgtat ttgcaaaatt aagcagagga aaaaatagtc aaaagatgca
     7921 agaaaaagat agtcctatat ttgataaggt aagtgaagca tataaaaaat tattaagtgt
     7981 atgtttgaga catcaacgct tagttgtaat atcaagttta cttatttttg tagcttcctt
     8041 atttggaatg ggatttatag gtatggactt tatgcctgca gcagacaagg gtgaattatc
     8101 tatttctata gaacttccaa aaggtcttag cctagaatcc aatgattact atgtatctat
     8161 gactgagaaa aaagtctcag atattccaga aattaaaact ttaataacaa ctttgacatc
     8221 agattcaaat aatagtaaag caagtatagc aattgaatta gaatctcaaa aaaatagaaa
     8281 aagaactact gatgaaatag aaaaagagat aattgataga gtggctactg taccagattg
     8341 taaaataaat gtaagtcaaa acagtagtgt tatgggtgga gctggaggag cagacttcac
     8401 attagaaatg aaaggtcctg atttagatgt attaaaaata ctgggaaaag aagctgaaaa
     8461 agaatttagc tctataaaag gatttagaaa tgttgaaact tctcttacag atacaacaca
     8521 agaagctcaa tttactatag ataaacataa ggctcaagaa tatggtgtaa ataccgctga
     8581 aattgcaggc atgttacgta cagcaataaa tggtgactcc gtaaccactg ctactattga
     8641 tgattacaaa gtggatgtaa acctaaaatt taaagcagat actattgata gcttagaaga
     8701 tataaagcaa ctaaaagtaa actctgcaac tggaaaagaa gttcctcttg gtgctttttc
     8761 agatataaaa atggcagatg gtctacaatc tatatctagg actgatggag atttttctgt
     8821 aaatataact gctacacttg acaatttaga tgctggtact gctacaaaac tagcactaga
     8881 aaaaacgaat aaaatggtaa ttcctaaaga ttactctata ggtgttggtg gtgaaacaga
     8941 aatgatgaat gaatctatgg caagtcttgg attttcaatg gtcatagcta tagcattagt
     9001 ttatatggta atggtagctc agtttgaatc atttagtaaa ccatttataa tcatgttctg
     9061 tgttccattt gcattcgttg gagttgtgct ttcgcttcta gttagtggtc aaagtttaag
     9121 tactgtcggt atgctaggta taatcttact tatcggtatt gttgttaata atggtatagt
     9181 tcttattgac tacatagagc aacttagaaa aacaaatcgt aacaatgatt taattggttt
     9241 ggtttcaatt ggttgtgcag caagattaag accagtctta atgactacag caacaacaat
     9301 tttaggtatg ttgccaacag cactatctct tggtgaaggt ggagaaacta tgcaaccact
     9361 agccatagtt ataatgggtg gtctaactgt atctacactt ataactttgg tgcttatacc
     9421 tactatatac ttaatatttg ataaatggga aaataagttt tcagacaaaa tgcctaaaat
     9481 atttggtaag ataacagata aaataggaag tataaaacgt aaaataataa agaaaccaaa
     9541 attgaaagct aaagtttaaa tagaatggag ggtgattgta ttgaataaaa aaatgagcaa
     9601 aatagttgca attggctgtg gtgttggtat aataactgcc aatttaatcc cagtttacgc
     9661 agataataat acttcttcta taaacaaagt tgataattct attttaagtg actcaaataa
     9721 aagaacatct gaaaaggaaa ttttgactgc tagagaagct gttagtgctg cacttaaaaa
     9781 tagtgaaaaa ttaaaaatga aatcagaaga aataaaaatg ttaaaagaaa agttagaagt
     9841 acaagatgag tttgatagtt ttactggaag tgacaactcg tttccatacg accaaattgg
     9901 acttcttaaa aatcaaagtg aacaggctaa aggctttatg gaggaccaaa tagcaaatga
     9961 tattactaat aaatttaacg atttagtttc aagagaaaat gagctagaca agataaaaaa
    10021 caatcttgaa ataaaaacta aagaaattaa agatatgaaa ttaaaaaaag atttagggtt
    10081 agtaacatct ttagaaactg aatcagctga acttgaactt caaacactac aaaatactca
    10141 aaaagctaaa cttcaagaac taaaaaacaa tcaagactac tttaagttgt tgactaatat
    10201 agatttaaat aattatcaat tggataaaga atatagattt gaatctttta gagtaggtgg
    10261 ctcagttgat tcatatatgg aaggtaaggt taatgaatat ttaaaatacg accaattgat
    10321 tttagaacgt acagaagaaa gctttaatga taaagatgag aacaaagcag atttaccaga
    10381 tagacctaat cttacaagac cagttgctcc tacaaaacca gagcaaggta acctaactga
    10441 tgatgaatat aaggttctta cggataagta tgaaaaagac tacaagagat atgaaaaaga
    10501 agttgaagca tacaatcttg aaagacagac ttatgctgct ggccttacta cttatgcaaa
    10561 ttatcttcaa caaaaattta ataccgaaaa tggtctagta acattagaag acagtaaaaa
    10621 agctcttaaa aaagggttaa ttgatagcta tgctcaatta cttgctttag aagatacaat
    10681 tcagattact aaaaaacaac ttgatttatc agaaaaacaa ttaaaaaata ctaaattaag
    10741 atatgattta ggattaatta ctctaactga ttataaaaaa caagtggtaa gtaatgaaga
    10801 tgctaaaaat agctatgaca ctcttattgt gaattataac tcactaaaaa atggtataga
    10861 aaaaccttgg atacttaata ctggaaaata attctggtaa atagaaagat tactttaaca
    10921 tacgtttgat taaaaaatag ttatgcacac tcgtggcttt agtcatggaa gattcagaag
    10981 gcaaataata gaaatagatt agtaaaaata tatttgctta taaaactgta taattttata
    11041 gaggctatct caaaatagag ataagcctct tttttattgt ttaaattata aaaatatatt
    11101 taattccata caaaaagagt gccttatgaa ctaaaaagtt cattttgaga caccctcaaa
    11161 agcttaactc taaatttaat tattttttat caaatgtctc atctatttta catctcacca
    11221 tgttaacaaa ttcagaaata ttatatcctc taagtttgca tccacattta ggacaataat
    11281 ccaatttcct agataccaaa gccctacaac taggttctgg acatcttatt tttagactct
    11341 catctgctac tttaaatcca catcgtgggc atatactacc tggacgtgat gatacttcct
    11401 ttccacattt aggacacttt gtcaacttta tcccctccag taaactcaat ttaattgata
    11461 gttgaaattt ctctctacaa taatatatgc aatataatta aatacgtttc acttacaact
    11521 atataagtta aatttttata ataaaaatcc cctatattca atttacaaat acttaatttg
    11581 atacattgaa caatagggga ttttattttt atttagttac atattttgca gtctattatt
    11641 atctaggatt ttttatagat gcttgagctg ctgcaagtct agcaattggc actctaaatg
    11701 gtgaacaaga tacatagtta agtcctacac tataacagaa ctctactgaa gatggttctc
    11761 ctccatgctc tccacatatt cctagtttta attctggttt aacttctcta cctaatttag
    11821 ctcccatctt aactaactta ccaacaccat tttggtctag tacttggaat gggtcttttt
    11881 ctaatatttc ttcatctaca tattgaccta agaatttacc agcatcatct cttgaatatc
    11941 caaatgccat ttgagttaag tcattagtac caaaactgaa gaagtctgct tcttgtgcta
    12001 tttcatcagc agttaaacaa gctctaggta tttctatcat agtacctaca gtataaggaa
    12061 cttctatacc ttcttctttt attatagcgt caacagtttc tactatcatt tttcttatta
    12121 tttttaactc atttacttct ccaactaatg gaaccattat ctctggagaa actttaacac
    12181 ctgctttgat tgcttctata gcaccttgta ttatagcttt agattgcatt acacatattt
    12241 caggataagt tattgcaagt ctacaacctc tgtgtccaag cattgggtta aactcatcta
    12301 aatcaactat tctcttctta atatcactta cttttattcc catagatttt gaaagttctt
    12361 ctatagtctc atcatcatga ggcaaaaatt catgtagtgg tgggtctagt aatcttatat
    12421 taactggttt tccatccata actttaaata actctacaaa atcttctctt tgcattggta
    12481 atattttttc aagagctttt tctctttgtt ctactgtatt agataatatc atttctctaa
    12541 ctgctggcaa tctagcttca tcaaagaaca tatgctcagt tctacataga cctatacctt
    12601 ctgcaccaaa ctctatagct gcacgagcat ctctaggatt atctgcatta gttctaacca
    12661 tcatacattt gtgtttgtct acccagttca ttaatttttc aaaatttcca gttaaagata
    12721 cttctgtttt cttaacatct cctaggtata catttcctgt agaaccatct atagaaatgt
    12781 attctccttc ttttataact acatcatcaa gtgctcttat ttctttatta aactcatcaa
    12841 cttttatttc tccacatcca gcaacacaac atttacccat acctctagca acaactgctg
    12901 catgtgatgt cattccacct ctagctgtaa ggattccttc tgctttaacc attccttcta
    12961 tatcttctgg agatgtctct agtctaacta aaactacttt ttctcctttt tcatttgctt
    13021 ttactacatc atcagcattg aaataaactt taccacttgc tgcacctggt gatgctggta
    13081 aaccttttgc tataacctta gcttctttta atgccttatc ttcaaatttt ggatgtaata
    13141 attggtctaa ttgattaggc tcaattctca tgatagcttc tttttcatca attattccag
    13201 cttcaactaa atcaaccgct acatttattg ctgctgctgc tgttctctta ccatttctag
    13261 tttgtagtat atataatctt tcattttcta tagtaaactc tatatcttgc atatctttat
    13321 aatgaccttc aagtatatga gtaattttta caaattcatc gaatacagct ggcattacat
    13381 ctgcaagagt tgatatattt tgaggagttc ttatacctgc aacaacgtct tcaccttgtg
    13441 cattcattaa aaattcacca aataatttat tttctcctgt agctggattt ctagtaaatg
    13501 caacacctgt accactagtt tctcccatat taccaaatac cattgattga atatttactg
    13561 ctgttcctaa gttatgagct atatcattca atttacggta tacgattgca cgaggattat
    13621 tccatgatct aaatacagcc tctattgcaa gcattaattg ctcttttggg tcttgtggga
    13681 aatcattttt tatttctttt ttataaattg ctttaaattc ttttactatt tcttttaaat
    13741 catcagttgt aagttctgta tcaactgtgt atccctttgc ttcttttact ctgtctaata
    13801 cattttcaaa tttatatttt ggaacttcca tagcaacatc agaaaacatt tgtatgaatc
    13861 ttctgtaact atcatatgca aatctttcat tttgtgtagc ctctgctaag ccaacaacac
    13921 tattgtcatt aagacctagg ttaagaattg tatccatcat tcctggcata gagaatactg
    13981 caccagaacg aactgatact aataatggat tcttattgca tcctaatgtt ttatttaagt
    14041 ctttttctaa agtagctaaa tgctcttcta tttcttttat tatctctgct cttatacttt
    14101 cattgttaac ataataatca ttacatgctt ctgttgttat agtgaatcca ggagggacag
    14161 gtaagcctat cttagtcatt tctgctaaat tggcaccttt acctcctagt aaagatttca
    14221 tgtctttgct tccttcacca aaactataaa catacttagt ttccattgtt aaatccccca
    14281 ttaatctttt tattttaatt tatatcattt ttattaatag acattattaa atttctattt
    14341 ctttgtatat ctttagacca ttttctttca ttatgtctaa aattatacct gctgtttctt
    14401 ctattgcctt atttgaaacg tttattactg ggcatccaat tcttttcatt atctcttctg
    14461 aataatctaa ctcctgaagt attctttcta aattagcata gtttgcttta ctggacaatc
    14521 ctaaagcttt taatctttgg gttcttatct cattcagctt ttcaggtgaa ttagtaagac
    14581 ctattatctt tttagtatct atctcaaaca cttctttagg aattggtatt tctggaacta
    14641 atggaacatt agctactttt atatttttat tagctaaata catactcaaa ggagttttag
    14701 atgttcttga tattcccact aaaattatgt ctgcttgaag aacacctcta gggtcttttc
    14761 catcatcata tttcactgca aattctatag cttcaactct cttaaagtaa gattcatcta
    14821 attttctaat tataccagct tctcttttag gttttcttcc tgttctcttg cttatttctc
    14881 ttagtatatc tgtcattaaa tctatacaac taagaccttc tctttcgcaa tatctttcaa
    14941 caatactgca taattcttca tcaactaatg tatatacaat taaagcgttt tcagcttttg
    15001 cattctccaa agtttcaagt agtactcctt tattaatcat gtatggaaat ctttttattt
    15061 cataagtttc attcacataa aactgagaca tacatgcctt tgcaacttgt tgtgcagtct
    15121 cccctactga atctgatact gcatatataa ttaggttttt caccagcata cctccttaat
    15181 tgtcaactat ttctaagaat agcttagtta tgttggtttt tgatattctc ccaacaactc
    15241 tcatatgatt tttgtcttct cccttatatt caacaatcgg aatcgaatct acttcatgtt
    15301 ctattatttt tcttgaagct aataaaacac ttgctccttt agttaaagtg actacatttg
    15361 gagttcttgt cattatcata cctattggca ttttatttat atcagaacct cctatagttg
    15421 cttttagcaa gtcttttctg gaaacaactc cacataactc ttcattctca tctataataa
    15481 ctatactccc tacatcagat aaaaacatag tgactattac atcatatata ctttcatcct
    15541 tcttagctag tacaggcatg ctcattatat cctctactgt tttttctttt acacttttac
    15601 caattaaatt tatttcacta actcctgaat aaaaatatcc aacttttggt cttgcgtcta
    15661 atattcctgt cattgttaat atagctaaat cagatctaag tgtagcacga gtcacattca
    15721 agctggaggc aatactttca ctcgttattg gttcattttc cttaactata tctataattt
    15781 ttagttgtct ttcattaagc tgaataataa tcaccttctt tcattgtgct atacttttta
    15841 tcttttcgca tcttatatag tatattatat aactatatat accattctgt aaacagattt
    15901 tttattgaat acgatatatt ttttactaag ttctctcgct taaagatatt tattatatat
    15961 taatggggga tatttcttta attaatagaa ctatattagt attacagt
//
LOCUS       DAYAOX010000085        15990 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.085, whole genome shotgun sequence.
ACCESSION   DAYAOX010000085 DAYAOX010000000
VERSION     DAYAOX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.085"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            101..1549
                     /locus_tag="ACGI57_002034"
     CDS             101..1549
                     /locus_tag="ACGI57_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFG9717112.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKN
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            1575..3965
                     /locus_tag="ACGI57_002035"
     CDS             1575..3965
                     /locus_tag="ACGI57_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFG9717113.1"
                     /translation="MSLIFTTKDQLVELGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGVRTYFEATNDEEFMMQNGLEILIETSRFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVFVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            3967..4638
                     /gene="pgmB"
                     /locus_tag="ACGI57_002036"
     CDS             3967..4638
                     /gene="pgmB"
                     /locus_tag="ACGI57_002036"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFG9717114.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     INREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGVKDEFDCIVNPSLLKRGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            4677..6047
                     /locus_tag="ACGI57_002037"
     CDS             4677..6047
                     /locus_tag="ACGI57_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9717115.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIENFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            6394..7236
                     /gene="licT"
                     /locus_tag="ACGI57_002038"
     CDS             6394..7236
                     /gene="licT"
                     /locus_tag="ACGI57_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFG9717116.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            7381..9309
                     /locus_tag="ACGI57_002039"
     CDS             7381..9309
                     /locus_tag="ACGI57_002039"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9717117.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            9370..10797
                     /locus_tag="ACGI57_002040"
     CDS             9370..10797
                     /locus_tag="ACGI57_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFG9717118.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFVVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            11208..11984
                     /locus_tag="ACGI57_002041"
     CDS             11208..11984
                     /locus_tag="ACGI57_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9717119.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            12000..12365
                     /locus_tag="ACGI57_002042"
     CDS             12000..12365
                     /locus_tag="ACGI57_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFG9717120.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWRSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(12541..13290)
                     /locus_tag="ACGI57_002043"
     CDS             complement(12541..13290)
                     /locus_tag="ACGI57_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFG9717121.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELIST
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFEIITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            13512..13640
                     /locus_tag="ACGI57_002044"
     CDS             13512..13640
                     /locus_tag="ACGI57_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717122.1"
                     /translation="MKDYKYKGYFEGVEIFDNVNDLNLNTLTTVSRFVFVFLFTIT"
     gene            13768..13950
                     /locus_tag="ACGI57_002045"
                     /pseudo
     CDS             13768..13950
                     /locus_tag="ACGI57_002045"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            13978..14193
                     /locus_tag="ACGI57_002046"
     CDS             13978..14193
                     /locus_tag="ACGI57_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFG9717123.1"
                     /translation="MVPPKGKFFFGAVVVGERGQIVIPKEAREVFNINAGDKLLVLGD
                     EERGLGIVHQRDLINFIGHVGVVHKEE"
     gene            14225..15412
                     /locus_tag="ACGI57_002047"
     CDS             14225..15412
                     /locus_tag="ACGI57_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide family glycosyltransferase"
                     /protein_id="HFG9717124.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            15604..>15990
                     /locus_tag="ACGI57_002048"
     CDS             15604..>15990
                     /locus_tag="ACGI57_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HFG9717125.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLK"
BASE COUNT         6277 a         1688 c         2839 g         5186 t
ORIGIN      
        1 ttttaaattg aattatttaa aaggtatagt atgaaaaaaa tcttataact aaatatgaga
       61 tagaaattat aaaattttat actgtgaaag aggtagatgc atgaaattag ggaaagattt
      121 tttatgggga ggggcaattt cagccaatca aagtgaagga ttttatttag atggaggaaa
      181 aggtttatct cagtttgata aactcccatt aaatgataag agattaaaaa atgtatgttt
      241 agatgaggat aatattttaa atgaaaataa tcaatactat ccaagtcata tagggattaa
      301 attttgtgaa aattacaaag aagatatagc aatgttagca gaaataggtt ttaaatgttt
      361 tagattttct atatcttggt caagaatatt cccaacagga gaagaaagtg aaccaaatga
      421 aaagggatta caactttatg ataatataat aaaagaatta aaaaaatata atatagagcc
      481 attaataacg ataagccact ttgatatacc ttattctcta gttgaaaaat attctgggtg
      541 ggctgataga agagtggtta atttatatgt aaaatatgct gaaattttga tgaaaagata
      601 taaagatgat gtcaaatact ggattccttt taacgaaatg aatatgataa tgcatattcc
      661 atatataggt gctgggttaa cttttatagc tgatgaaaat caacttcaga aaaaatatca
      721 agcagcacac catcaattat tagctaatgc aaaaactata gaaataggaa ggtatataaa
      781 taaagaattt aagtttgggt gtatgttagc tgctggaaaa acatatccat acacatgtaa
      841 accagaagat gtatttgcaa ctttagagca tgagaggcat aatttgtttt tttctgatgt
      901 tcaagttagg ggtgtgtatc caagctttat gagttattat ttaaaatcaa atcaaataaa
      961 attaataata aaagaggaag atttatcaac acttaaggat aatactgtag attttgtatc
     1021 ttttagctat tattctagtg cttgtagttc agctagagct gaaggtttag aaaaatcaaa
     1081 ggcaaacgga ccagaaacca taaaaaatcc atatcttcca aaatcaaata gtgtatggca
     1141 aaacgaccct gtagggttaa gaataactat gaatcagtta tatgaaaggt atcagttacc
     1201 tttatttata gtagaaaatg gactgggtac tcaagatatt gtaaatgaag atggaaaaat
     1261 aattgatgat tatcgtatag attacatgaa agaacatatt aaaaatatga ttcttggagt
     1321 aaatgaagat ggaatagagc ttataggata tcttatgtgg ggatgtatag atgtaaccag
     1381 tgtaagtgaa ggaaagatga caaaacgata tggcataatt catgttgatt ctgatgacta
     1441 tggaagaggc tcatttaaaa gaactaaaaa acaaagtgct ttttggtata aaaaagtaat
     1501 agaaacagat ggagaaattt tatttaatga tatggaaact catgcataat tcattttaag
     1561 aaaggagtgt tattatgagt ttaatattta ctactaagga ccaacttgta gagctagggt
     1621 ttagtaagga tacaataaaa tccaatgaaa cgttgtttac actagctaat ggacatttag
     1681 gagttagagg gaatattgaa gagtctgatt ttaataatga gtatgtagat aatatgggta
     1741 catatgtaaa tggattttat gaagaatctc caattacata tggagaaagc gcttatggct
     1801 atgcaaaaca aaatcagact atctgtaagt tacctaatgc tcatatagta aatttctcta
     1861 ttgaaggaga ttgctttgat ttatcaaaag gtattacaag tgaacatact aggatactag
     1921 atttaaatga agggattctt aaacgttcat ttatatggga aaactctcgt ggtagaaaag
     1981 ttaaagtatc tatagaaaga ttagtatcac aagatataga agaattaatg atgatttcat
     2041 atacaataac accattgaat tttaatggaa aaataaaatt taaaaataaa atggaaaata
     2101 gtatggagaa gtttaattgt gaaaaagtag atgacccaag agtggcatct ataaataaga
     2161 agcaatttaa aattaatgta aaaaaatata acaataagta ttttatgaat ttagttacag
     2221 aaagaagtag attagggtta tggtgtgggt ttgattcata tattacttgt ggaaatatta
     2281 caaatgagac ctataatgtt tcagatgaat gtattgagcg agaaatagaa gtctatgcca
     2341 aaaaagatga aaggttaaat ttagaaattg ttgtaggcta tagtagagta tattctgatt
     2401 taaatctaaa tgaagaacgt ttaaaatcat tagaatgtct ggagtcgtta aaatgtttag
     2461 aatcagaagc acatttaaaa tcattagaat tggttttaag agaagcatgt aatattggat
     2521 ttgataaagt caaaaatatt cagagaaaga aaatgaatga gttttggaca acctctgatg
     2581 tagagattga aggcgataaa actcttcaac tggggataaa aaccaattta tttcatattt
     2641 atcaatcagc aggaagagat ggaaagacta atatatcagc aaaaggtctt acaggtgatg
     2701 gctatgaggg gcattatttc tgggatacag aaatgtatat attaccattt tttatatata
     2761 ctcaaccatc cattgctaga gctttattag attatagata ctcaatctta gatgcatcaa
     2821 gagaaagagc tagagaactt ggaataaata aaggttgctt atattcatgg agaactataa
     2881 atggtaatga atgcagtgca tattatccag caggaacagc acagtttcat ataaatgcag
     2941 atatagctta tggtgttaga acgtattttg aagcaactaa tgatgaagaa tttatgatgc
     3001 aaaatggctt agaaattctt atagaaacat caagattttg ggttgaattt ggagattata
     3061 tagaatcaaa aaataataaa ttctgtataa atggagtaac aggaccagat gaatatacag
     3121 caatagtaaa caataattac tacactaatt taatggctaa gtataattta atatatgcag
     3181 tacagatggt agataaattt aagcataatt tattttcaaa tgaagtattt agaaaagtta
     3241 aattacaaga atatgaagtg aaaaactgga taaatgccgc tcaaaatatg tatttaccat
     3301 atgatgaaga gaagaaaata agcaaacaag acgattcatt tttagaaaaa tcagtatggg
     3361 attttaaaaa tacaccaaaa gaaaaatatc cattacttct ccattatcat ccattaacaa
     3421 tatatagata tcaagtaaat aaacaagcag atactgtact ttcagcgttg cttttccctg
     3481 atgagtttag cttagaacaa aagaagagag attttgaata ctatgaaaaa ataactactc
     3541 atgattcatc tttgtcaagg tcaatatttg gaatgatggc aagtgaaatt ggaaatcatg
     3601 aaaaagctta taactatttt atggacacag cattaatgga tattaataac ctacaaaaaa
     3661 atacaagtga tggaatacat gctgctaata tgggtggaac atggatgagt ttagtatatg
     3721 gatttgcagg aatgaaaata aaaaatgata aattaagttt tgaaccaagg atacctaaac
     3781 actggaaaaa tattaaattt aatatattat ttagagataa tttagttttt gtaaatatag
     3841 aagaatataa atcttattat aagttaatca aaggtagaaa tataaccata ttacatgata
     3901 ataaagaaat aaaattatca aagaacataa tagaagttaa taattgtttt aaagggagta
     3961 attaatatga aagcagtctt atttgatctt gatggagtta taacagatac tgctaagtat
     4021 cattttgaag cttggcatga gatagcaaaa tcgctagata tagaaataga tgaaactttt
     4081 aatgaaaaat taaaaggcat aaacagggaa gaatcattag ataaaatatt gttatctgct
     4141 aataaagtag atagttatac taaagaggaa aagttaaagg tgtgtgaaga aaaaaataac
     4201 atttatataa agttaataaa ctcattaaca tataaagata tattaccagg gataaatgag
     4261 cttataaaag aacttaaaaa aaataatgtg aaattgggaa tagcatctgc tagtaagaat
     4321 gcacctagga tattagaatc tttgggagta aaagatgaat ttgattgtat agtaaatcca
     4381 tctttattaa aaaggggaaa accatatcct gatattttta ttgaagggag caagatgcta
     4441 aatttaaaac aatatgattg tgtaggaata gaagattctt attcgggtat taaagctata
     4501 aatgctgcca atatgacatc aattggtgtg gggaaaaaag aagtactagg aaaatgtgac
     4561 catattgtag aagatactag cttattaaat tacaatacac tattacaagc ttggagtaaa
     4621 aagattaatt tggattaaaa ttatattaaa aattatttta aaagagagga taaggtatga
     4681 agaataaata tgaagatgta gctcaattga taattgatgg acttggagga aaagataata
     4741 taattgactt aactcattgt atgacacgat taaggttcat cttaaaagat gaaaagaaag
     4801 tcaatgcaga taaattaaaa tctatagata aggtagttgg agtaaactca acttcaactc
     4861 aatatcaggt cataataggt aatgaggtag gtgttgtatt taatgcaata ataaataagg
     4921 ggataaatac cacaggagat attaaaagcg atataaaaaa taaaaataaa aaaaatagta
     4981 ttttaaataa tataatagat acaataactg gttgtatgac acctatgata ccagccctta
     5041 cagcatctgg gatgataaaa gttatattat ctctttgcac cacttttaat tggctatcat
     5101 caaattcaag tacatataga gtattagata taataggaga tgtagcattt tactttatgc
     5161 ctataatact tgcaataaat gcagctaaaa aatttaatgt taatacatca atagcagtta
     5221 tagtagtagg tgtgttttta catccaaatt tctctgcatg ggtatcaagt ggggacccta
     5281 tatcatttat aggagtacca attcaaggtg ttatatatgc agcatctgtt atccctgcac
     5341 ttttaacagt atggatgatg tcatatatag aaaatttcat tgataaatta acacctagca
     5401 tgttaaaaac aattttaaat ccaactttag tattattaat aagtgcgcca atagcattaa
     5461 tagtaattgg accaatagga aatttcttag gagagggatt agctagtata ataaatttac
     5521 tacaaggaag gttaggattt ataatggttt gtttattagc agctgcaatg ccatttatcg
     5581 ttatgacagg gatgcatcat gcattgactc ctatattcat atcagcattt gcaactacag
     5641 gacaagaatc gctaatatta atatctcaag tttgtgctaa tttagcgcaa ggtggcgcaa
     5701 cattagctgt agcattaaga agcaaagata gtaagatgaa acagttagca tcagctgcat
     5761 ggatatcagc tattatgggt ataactgaac cagctcttta tggagttaca ttgaaactta
     5821 aaaaacctgc tatgttagca tctataggag caggaatcgc agggttattt gcaggaatta
     5881 ctcatgtgac tttatatgtt ccacaaaata gtataatggc agtacttgga ttatcagggg
     5941 aaaaggggat ttcaaatata gtaaatggac ttatagttat atttatagca ttggttgctc
     6001 catttattat gacatatgtt tttggagttg aaaaaaatgt taaataaagt acagataatg
     6061 tagtagagga agtataatat taactattta cattaataga atttttatta ttattgacta
     6121 taacttgttt tgtatttata ttgatgcaaa gaaataagtt aatattaata agaataatta
     6181 ttataactga caaaaagcaa cataaaagat tgatgaagta tatggaaata aaaagttgac
     6241 aaaatatgtc tgatgtttta tcatattaat tggaattagg tagtaattgt ttaagtacaa
     6301 gctaagctta ttcattaata atagcagtat cctaggaagg agtatgccca ttattatgga
     6361 taagcttttt ttatgcttaa aaggaggata atcatgataa ttaataagat attaaacaat
     6421 aatgttgtaa tcacattaga tgataatgat gaagaagtta tcgttatggg taaaggaatt
     6481 ggttatcaaa aatcaaaagg caatctaata gataaaacta aagtaaataa agtatttaga
     6541 atatcaaata aagaaatatc aaacaaacta caagaattac tcaataatat tcctatagaa
     6601 cacatgaaat tatcaagtga aattatagaa tacgcacaaa taaagttaaa caagaagcta
     6661 aatgaaagta tttacatatc cttaagtgac catacatatt ctgcaattca aagaatgaaa
     6721 gaaggaataa atgtaaaaaa tgcaatcctt tgggaaatta aaagatttta taaagaagag
     6781 tttgaaattg gaatgaaagc actagatatt attgaaaaca aaacaggtat aaaattacca
     6841 gaagatgagg ctggatttat agcctttcat atagtgaatg ctcagttaag tgaaggtcat
     6901 actttggcat ctgatattac gaagttaata caagaagtac ttagcatagt taggtatcat
     6961 tttaggatag aatttcatga agaatcagtg ttttattata gatttataat gcatttaaag
     7021 ttttttgcgc aaagattgct tttagatagt gcacatgaag gtgagacaga caaagaatta
     7081 ctaagcatta ttaaatctaa atataataaa gagtttgaat gtgtagttaa aattaaaaat
     7141 ttcattaaaa aacaatataa ctacatatta acagatgatg agcttatata tttaacaata
     7201 catttagcta aagtagttaa agactcaaat atgtaagtga aataattttt cagcagtata
     7261 ttaagaatga ttgaaaggag tgatactaac aaaattatac ttatggttgg atggtaacat
     7321 tgagttggcc aaaccttcaa atttcaaaaa attaatttaa acgaaatatg gaggagaaaa
     7381 atgggaaaat acaaacaatt agcacaagag atagtaaaca atgttggtgg aaaagaaaat
     7441 attagtggat tagtacactg tattacaaga ttacgtttta ctttaaaaga tgaaagtata
     7501 gcaaatgaca atgtattaaa aaacatggaa ggtattgtta ctgtaatgaa aagtggagga
     7561 caatatcaag tagttatagg aaatcatgtt gaagctgtct ataaagatgt agttgaaata
     7621 attggtttag atgattcaag tacaagttca gaaactaaaa aatcaggtaa tatactagat
     7681 aaaggtatag atataatatc aggaattttt cagccagtgt taggaataat ggcagcttgt
     7741 ggtatgttga aaggatttaa tgcattattt gtagcaatgg gattatatag tgatgtatct
     7801 ggtggttata tggtaataaa tgcagcggga gatgcattat ttacattcct tcctttgttc
     7861 ttaggatata cttcagctaa gaagtttgga ttaaaaccaa tgttgggatt agcactagga
     7921 gctgcagtat gttatcctgc tatacaaggt agcagtatat cagcaggagc agatgtgatg
     7981 tacactttat ttaaaggaac aatgtttgca tcaccagtat atattgattt ctttggacta
     8041 ccaattgtat caattgatta tactggaaca gtcgtaccaa ttatatttat agtgtatttt
     8101 gcttctaggt gtgaaaaatt atttaataag tgtgttccaa atttagttaa gtttttcttt
     8161 gtgccaatgc ttacattatt aataacagtg cctgttgcac ttatagttat aggccctata
     8221 gcaacatttg gttctacact tatttcacaa attgtaattt caataagaga ttttagtcca
     8281 ctattggcag gtgctatagt aggatttact tggcaaattc ttgttatttt tggtatgcac
     8341 tggggattta ttcctgtata tataaacaat gttatgactt taggatatga taatgttatg
     8401 atgccattct ttgcctgtac atttgcaagt tcggcagttg tgttggcaat atttttcaag
     8461 actaaagata aaaaactaaa agagatggca ataccaaact ttatatctgg tatatttgga
     8521 gttacagagc cagcaattta tggaatatta ttgcctttaa agaaaccatt tataataagt
     8581 tgtattgcta gtggtattgg aggcgctttc tatggattct ttaatcttag aaaatttata
     8641 actggaggaa tgggaatatt tgaacttcca gcaatgattg aaccagatgg gggaatgggt
     8701 aacttaatag tagctctttc tggtatagct atatcaatgg tcgtagcttt tgtattaact
     8761 atgattttat ataaagatga gaaagtagaa gagtctaaaa aagtaagaaa taagggtaaa
     8821 gaagaaataa aagaagtcaa gtctactaaa ttagaaagag aaatagtaac aagtcctata
     8881 aaaggtgaag tattaaaatt aagtgatata gaggatgcag catttgcatc tggtgtacta
     8941 ggtcaagggg tggcaataat acctagtgat ggaaaagtag ttgctccagt agatggggta
     9001 gttacaacct tatttcctag tctacatgct atagggattc tttctgatac aggtgttgaa
     9061 gtgcttattc atattggatt aaatactatt caattagagg gaagaggttt tgaggcatgt
     9121 ataaaacaag gtgatagaat tacaaaaggt caaactatat taaactttga tgttgatgca
     9181 ataaaagagt tggggtatag tactgtaact ccaattgtta ttacaaattc atcacaattt
     9241 ttagatgtag tagaaacaga aagtaaaaat attgaattag aagacaatct aattacagtc
     9301 ttattttagt aaagattgag gtattagcat aatctagtgc tttggttata attttaggag
     9361 gctttgacta tgagttttaa gaatgatttt ttatggggag gagctactgc ggccaatcaa
     9421 tgtgaaggtg gatacaatga agacgaaaga ggattggcaa atgtagatgt atgtccaaca
     9481 ggaaaagata gaacagcagt tattacaggg aaactaaaaa tgtttgattt tgatgatgag
     9541 cattattatc cagctaaaac tggtattgat atgtatcata attataaaga agatataaag
     9601 ttatttgctg aaatgggatt taaagtatat agaatgagca ttgcatggag tcgaatattt
     9661 ccaaaaggtg atgaagaaac accaaatgaa aagggacttc aattttatga aaatatattt
     9721 aaggagtgta aaaaatataa aatagagcct ttagtcacta ttacacactt tgattgtcca
     9781 atgcatttag ttaaaaagta tggtgcatgg cgtaatcgaa aaatgatagg tttctatgag
     9841 aaattatgca atgttatctt tagaagatat aaaggattag tgaagtactg gctaactttc
     9901 aatgaaataa atatgatttt acatgctcca tttatgggtg ctggtatttg ctttgaagaa
     9961 ggtgaagatg ttgaaaagat aaaatatcaa gctgcccatc atgagcttgt agctagtgca
    10021 attgcaacta aaatagcaca tgaagttgat tcaaataact taattggatg tatgtttgca
    10081 gctggaagtg tatatcctta tagttgtaat cctaatgatg tatgggaagc tacaaagtta
    10141 gatagagaaa attacttctt tgtggatgta caatctaaag gtaaatatcc taactatgcg
    10201 ttaaagtata tggagcaaaa aggtattaca ccagaaatgg aacctggtga tattgaatta
    10261 ttgaataaat atacagtaga ttttatttca ttttcatatt acaatagccg ttgtgttaga
    10321 accgatgaaa atgcagatga tatggctgaa ggaaacatat ttacatctgc taaaaatcca
    10381 tatctttcat acagtcaatg gggatggcca attgacccat taggtttgag aattacacta
    10441 aatcatgtat atgatagata tgaaaagcca ctatttgttg tagaaaatgg attaggtgct
    10501 aaagatatag ctgatgaaaa tggttatgta gaagatgatt acagaattga ttatataaga
    10561 gaacatataa aagctatgca tgatgcagtt tctatagatg gggtcgattt attaggttat
    10621 actacatggg cacctattga tttagtaagt gctggtacag gtgaaatgga aaagcgttat
    10681 ggatttatat atgtagatag agacaatagt ggtaatggaa gtctaagaag aatgaaaaag
    10741 aaaagttttg aatggtataa gaaagtaatt gcttcaaatg gtgaagattt atattagaca
    10801 agaaatctat gttaaataag aaatttgtat taaataagaa gagtataaaa attaataata
    10861 agtataattt ttaacttttt taactataga aaatcaatac agaattattt tacattgtat
    10921 ttatgcaata gtatgattca caaataaaat tttagaaaat aaaattgtta agaagtatgg
    10981 gaagtaagtg caatttactt tgtaataaaa gatgaaaaag gcaagcttag gttttaagct
    11041 tgtctttttt attatgtata aactatgata ctttaaaatt ctatgaaaaa tttatttaat
    11101 agagaaatta aaaatttaat ggtaatcaaa ttctttacca atgaatataa aaaatgtcta
    11161 cgtgttataa tagtttaaac taataaacga aaggtagatg aatgaatatg aaataccaac
    11221 atataataga agttgacaaa gaactttgca ttggatgtgg gctttgtaaa aatgattgtc
    11281 cagtaaacaa tataattata gaaaataaaa agtcagtaat taaaaaacaa gattgtctaa
    11341 tgtgtggaca ttgtgcagca atatgtccca caaaagctat taccttgaca ggatttgatg
    11401 aacctcctat agaacttaca aataagccaa agctagattc agatgaatta ctaatggcaa
    11461 ttaaatctag aagaagtata cgtaaattta aagacaaaga agtttcttca gaaattataa
    11521 agcaaattat tgaggcaggt aggtataccc ctagtgctaa aaattcacaa gatgtatcat
    11581 atattgtttt agacaataaa aagtcaattt atgaaaacga agcagtgaaa ttttttagaa
    11641 aaataaaacc tattgcaaat atagctatta aatattcaaa agaagttgag attgacgata
    11701 actttttctt taagcatgct cctattgcaa taatgataat tacaaaagat aagattagtg
    11761 gttcacttgc agcatcaaat atggcattaa tggcagagtc ttatggccta ggtgttttat
    11821 ttagtggata tttttcagat gtagcaaata attcgccaaa attaaaaaag cttttaggtc
    11881 taaaacgcag taatcatgtt cttacaacat tagtaattgg atacccagat gttaaatata
    11941 gacgtactgc acagaaagaa gtagcaactg ttagatactt ataagagagg gtggatttta
    12001 tggaaaaaga atgtcaacaa agaatgcaag aaataacgtc taaatttaaa gaatgtcgca
    12061 aagctatctc tgctatgggt gatgagacta gacaattgat tttaattgct ctcttagaga
    12121 gtgattttaa tggtatacga gtgggagaaa tcacaaaaaa aacacatctt tctcgtcctg
    12181 ctgtatctca tcatcttaag atattaaaag aagcagagat tgttaatttg cgtagagaag
    12241 gaacaaaaaa ttattattat cttgattcaa aaaaatcaca atggaggtca ctaacagagc
    12301 taataaatct tatatttgtt ggaatacaac atattagtga aaatgatata agaaaaggtg
    12361 attaaattat acttgattta atacaaaaag attgatttag gaactaaaaa gaactaaaaa
    12421 gttcactttg agacactatc ttttgaacta tacccatctt aggagacagt taaaatatgt
    12481 aaaactgtat aaaacaatgg gtatagttca ttttataatg gtttaaatat taagaatata
    12541 ttattctaaa tagtatcttt ctctgttttg agttttattt ttatactcaa tagcttcagt
    12601 ggggcaacca caaatacatg ccatacaatg agtacagtta tttttccaaa ctggtttttt
    12661 gttttttaaa ttaatattat ttaaaggaca tagctctaca cattttccac agccaatgca
    12721 tttatcagta taatgaaatc ctttggcact aataattgtc ttataaaaaa ctgtatttat
    12781 tataccactt ttaattgtac ctcctaagcc actaggagta ataatttcaa attcattttc
    12841 atttcgtata tcagaaataa tcctatatat ttgtttatct gcttctttaa tcatttgttt
    12901 tgatgtttct ttgtctgttg tagaaaataa agctatataa ttctctggca ttttaatttc
    12961 tgccatacct tttaattgcc attccttgta tttacatagt ttttgaatat agtttatgga
    13021 ttttgctgta tctccaccac aagtcattac aaagtaaact ctgttgcttc ctagaaattt
    13081 tgtttctttt ataaaatccg taaccactat tggcaatctc catgcataag taggacatac
    13141 aaatatgaat ggtttatctg ttgatattaa ttcatctgtt ttttcctctt ttataagttg
    13201 atttagtgat atcagttcat catttaattc ttgtgcaatt ttacgagcca catatcggct
    13261 attaccagtt ccgctaaaat ataaaatcat aataatatct ccctaactat tttttatttt
    13321 aactcttaat tttttattta ttatagtaat ttattgatat attctattca aatgtatgta
    13381 aaatttagta gttaagaaaa ttataacaca ttagtaattt ttatacttat tggtaaagaa
    13441 tttgattacc attaaattta gcaacaagac atcaactgga cttgtatatg agaagggata
    13501 ataatttgct tatgaaagat tataaatata aaggttattt tgaaggtgta gaaatatttg
    13561 ataatgtaaa tgaccttaac ttaaatactt taacaacagt tagtagattt gtgtttgttt
    13621 ttttgtttac gataacttaa ggaaatatgt tacctgaaca ataaatttat ataataaaag
    13681 tatataaata tatatatgaa tgttaagatt agttgataga atttaaataa taaatccaat
    13741 attataaaca taggaggtat gaaaaatatg attaatgaga atttaaaaag tctaagaaaa
    13801 atcaatcagt atacacaaga agaattagct gaaaaactta atgtttctag acaatcaata
    13861 gcaaaatggg aaagtggaga atctattcct gatataggta gttgcattaa acttgcaaaa
    13921 ttgtacaatg ttaaactaga tgacttagtt aatcattcgg aagaaaaaac aggtattata
    13981 gtacctccta aaggaaagtt tttctttgga gcagttgtag ttggtgaacg tggtcagatt
    14041 gtcattccaa aagaagcaag agaagtattt aatataaatg caggagataa attattggta
    14101 cttggagatg aggaaagagg attaggaatt gttcatcaac gtgatttgat aaattttata
    14161 ggtcatgtgg gagtagtaca taaagaagaa tgatttaaaa aaattataaa aggaggtatg
    14221 aaatatgagt aaaatagtat tttttagcat tcctgcatat ggtcatacta atcctactat
    14281 tgaagtagtt cgtgaattag tggatagagg aaatgaggta ttgtattatt cttttaatga
    14341 atttaaagat aaaattgaag gtgctggagc aaaatttatt tgttgtgata aataccttcc
    14401 agaattactc cctggagatg agaaaaaaat tggtaaggat tttcctggat taattgagat
    14461 gatagtagat actactattt ctttagatga aaaagtttgt agagaactta aagattttaa
    14521 tccagattgc attgtatcag attcattaag tttttgggga aagttatttg cacaaaaact
    14581 taatattcca tatgtatgct ctacaacaac ttttgcattt aataaacata cagcaaagtt
    14641 gatgaaacaa aactttatag aaattatacg tatgattttt ggtgtaagac gcattaataa
    14701 aaaaataaag cttttacaaa gaaaagggta tgaagtaaaa aattttattt ctatagtttc
    14761 taatgataat gatacggata ccattgtata tacatcaaaa gaatttcaac caatggtaga
    14821 aaccttctct agtaagtatt catttgtagg tccatcagta tcaaagttga ttattgaacc
    14881 taaagagaga aaaagaaagc taatatatat ttcgcttggt acaattaata ataaaaatgt
    14941 tagcttttat aaaaactgta taaatgcatt taaagattct gatgttgatg tgattatgtc
    15001 tgttggtaga agtactgata ttaaaagttt aggaaatata ccaaataatt ttgaggttaa
    15061 aaatagtgtt gaacagattg ctatacttca aaaaactgat gtgtttgtta ctcattctgg
    15121 gatgaatagt gttaatgaaa gtttatatta tggagtacct atggtattat ttcctcaaca
    15181 ttcggagcag agaatggtag ctaagagagt tgttcatttg ggagcaggaa ttatgttaaa
    15241 agaggataaa tcagaaagta ttaaaaaagc agtatttcaa gttataaatg acaatgaata
    15301 taaagaaaat gcaactaaac tatcaaaaag cttttataat gcaggaggct caaaaaaagc
    15361 agcagatgta atattacaga ttatatgtaa tagtagacaa gttgacatat aatagatttg
    15421 ttaaaaatat atatacatac atgtaattta atcaaagtac tgacagtggg gtcttttaac
    15481 ctaccagttg gggcatatat attgatgcta aatatgaggg atgttatatt ttaataaaaa
    15541 agataacatc ctttttttag tgcattatat cgagttttaa ataataaatg aaggaggatt
    15601 aatatgaaaa aatataaagt tatttttata gtattaatta taggtattat aagtgtaaca
    15661 gggtttgttg tatataaaaa ctgcattaaa ccatggaaag atgctgaaac atatattgat
    15721 aagtatatgg taaaacaaga tatttcaaaa gataatataa aaagtattac aaaagaaaaa
    15781 gccaagaagg caagttacga aggaatacta tacaaagtgt attacaaaga tgaccctcga
    15841 tatgagtatc aatattttta tagtgataat tattatgctc tatatgtaaa taaagtaatg
    15901 ttacaaattt atgatatgga aaataataat aaacttttaa gaagtaatga ggaattaaaa
    15961 agtgtaaagt atccaccaat atatcttaaa
//
LOCUS       DAYAOX010000086        15978 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.086, whole genome shotgun sequence.
ACCESSION   DAYAOX010000086 DAYAOX010000000
VERSION     DAYAOX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.086"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="ACGI57_002049"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="ACGI57_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFG9717126.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="ACGI57_002050"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="ACGI57_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFG9717127.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="ACGI57_002051"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="ACGI57_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFG9717128.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="ACGI57_002052"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="ACGI57_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFG9717129.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="ACGI57_002053"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="ACGI57_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFG9717130.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /gene="rplJ"
                     /locus_tag="ACGI57_002054"
     CDS             2662..3168
                     /gene="rplJ"
                     /locus_tag="ACGI57_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFG9717131.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="ACGI57_002055"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="ACGI57_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFG9717132.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3982..4770
                     /locus_tag="ACGI57_002056"
     CDS             3982..4770
                     /locus_tag="ACGI57_002056"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFG9717133.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5241..8957
                     /gene="rpoB"
                     /locus_tag="ACGI57_002057"
     CDS             5241..8957
                     /gene="rpoB"
                     /locus_tag="ACGI57_002057"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFG9717134.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            8999..12484
                     /gene="rpoC"
                     /locus_tag="ACGI57_002058"
     CDS             8999..12484
                     /gene="rpoC"
                     /locus_tag="ACGI57_002058"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFG9717135.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12773..13195
                     /gene="rpsL"
                     /locus_tag="ACGI57_002059"
     CDS             12773..13195
                     /gene="rpsL"
                     /locus_tag="ACGI57_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFG9717136.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13319..13789
                     /gene="rpsG"
                     /locus_tag="ACGI57_002060"
     CDS             13319..13789
                     /gene="rpsG"
                     /locus_tag="ACGI57_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFG9717137.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13838..15904
                     /gene="fusA"
                     /locus_tag="ACGI57_002061"
     CDS             13838..15904
                     /gene="fusA"
                     /locus_tag="ACGI57_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFG9717138.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6257 a         1989 c         3249 g         4483 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa ataatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactaa ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgaaag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaactga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
     3781 attgttgtaa tgtctgtata taaagtatat aattaaacta aaatgtaaaa ttttacattt
     3841 tagtttaaaa aaatacacga gtgtttataa ataaaatgta ataaataatt aattaacaaa
     3901 tataaatttt aaaataagtt agatatttag aaaaatcgaa atttgtagga tttaaaggac
     3961 atattcaagg aggagttaat aatggaaaaa ttacaaggaa aaattgcagt agttactgca
     4021 gcaacaaaag gtattggatt agcatcagca gagatattag caaaaaatgg agcaactgtg
     4081 tacttagcag ctcgttcaga agaattagct aatgaagtta taaataaaat aagtgcagaa
     4141 ggtggttgtg ctaagtttgt ttactttaat gctcgtgaag aagagacctt tacctcaatg
     4201 atagaagagg tagttaaaaa agaaggtaag atagatatac ttgtaaacaa ttttggttca
     4261 acaaaccctt ctcttgacaa agaccttgta actggggata cagataattt ctttgacaca
     4321 gtaaatacta atttaaaaag tgtgtattta ccatgtaaag cagcaattcc tcatatgata
     4381 aagaatggaa aaggaagtat agtaaatata tcaagtatag gttcagtatt acctgattta
     4441 tctagaatag cttactgtgt atcaaaagca gcaatcaact cattaactca aaacatagct
     4501 acacaatatg ctaaagacaa tgttagatgt aatgctgtac ttccagggct tattgcaact
     4561 aaagcagcat tagacaatat gtctccagag ttcataaaag aatttttaaa gcatgttcct
     4621 ttaaatcgta taggggaacc agatgatata gcaaaagctg ttctattcta tgctagtgat
     4681 gactcatcat ttataacagg ggatttactt gaagttgcag gaggatttgg tttacctact
     4741 ccacaatttg cagataatat attaggataa ttaaattact aatatgcaat gaaagcttat
     4801 gcattagtta tagtagaaat gatattttta taaaggccat agatataatt tatattggaa
     4861 aggtttgagg ttcaaaattc caagttaaat ttatttatgg tctttatttt gtatatttta
     4921 ataaattgta tatatttgta taaatgtaat tatttacaat cattttaata tggaattata
     4981 attaaatagg ggggatggta cttgtataaa attttttata gaaaattaga cctataaatg
     5041 aaaaatatgg attttatatt ggaaaattgt acataataca taatatatgc ttaaaataat
     5101 ttacaaaaaa tcaaaaataa tgcatagttt acatataaga taaaaaatat attgaaaaat
     5161 acttgacatt atgctaaaat tattagatgc gttagaatga actattgttt tatttttaat
     5221 tattgagagg tgaaaagtga atgccacatc ctgtcacgat aggtaaaaga actagaatga
     5281 gcttttctaa aattaaggaa atagctgatg tgccaaatct tatagaaatt caagtagatt
     5341 cctatgagtg gtttttaaaa gaaggtttaa aagaagtgtt tgatgatatt tcaccaatag
     5401 aagattatac tggtaatctt atattggaat ttgtcgacta ttctctagat gataaaccaa
     5461 agtacgatat agaagagtgt aaagagagag atgctacata ttgtgcacct ctaaaggtta
     5521 aggtaagact tataaataaa gaaacaggtg agataaaaga acaagaagtg tttatgggtg
     5581 acttcccttt aatgacagaa agaggaactt tcgttataaa tggagcagaa agagttatag
     5641 taagtcaatt agttagatct cctggtgttt actatgcgga agaaagagat aaaacaggta
     5701 agagattgat ttcatctact gtaattccta atagaggagc atggttagaa tatgaaacag
     5761 attctaatga tgtaatatct gtaagagttg atagaacaag aaaacaacca gttacagttt
     5821 tacttagagc tttaggaata ggaacagatg cagagataat tgacctttta ggagaagatg
     5881 agagattatc tgctacatta gaaaaagata atactaagac agtagaagaa ggtcttgtag
     5941 agatatacaa aaaattaaga ccaggtgaac ctcctactgt agaaagtgca tcatctttat
     6001 taaatgcttt attctttgac ccaaaaagat atgacctagc aaaagttggt agatataaat
     6061 tcaataagaa acttgcttta tgttatagaa taatgaataa aatttcagca gaagacataa
     6121 taaatccaga aactggagaa gtatttgtaa aagctggaga aaaaataagc tatgatttgg
     6181 ctaaagccat acaaaatgca ggtataaatg tagtaaatct actaatggat gatgataaaa
     6241 aagttagagt tataggaaat aattttgtgg atattaagtc tcacattgat tttgacatag
     6301 atgatttaaa cataaaagaa aaagtacatt atccaacttt aaaagaaatt ttagatggat
     6361 atagtgatga agaagaaata aaagaggcta taaaatcaag aattaaagaa cttataccaa
     6421 aacatatatt attagatgat ataatagctt caataagtta tgagttcaat atattctata
     6481 atataggaaa tattgatgat atagatcacc taggaaacag aagaataaga tctgtaggtg
     6541 aattgttaca aaatcaagtt agaattggtc tttcaagaat ggaaagagtt ataaaagaaa
     6601 gaatgacggt tcaagatatg gaagctataa cgcctcaagc gttagttaac ataagaccag
     6661 tttcagctgc aataaaagaa ttttttggta gttctcagtt atctcagttc atggatcaaa
     6721 caaatcctct atctgagtta acgcataaga gaagattatc agcccttggg cctggaggtc
     6781 tttcaagaga aagagctgga ttcgaagtgc gtgacgttca ccattcacac tatggtagaa
     6841 tgtgtccaat agagactcca gagggaccaa atatcggtct tataaactct ctaggaactt
     6901 atgcaaaaat aaatgaattt ggatttatag aatcaccata tagaaaattt gataaagaaa
     6961 catcaacagt tactgatgaa atacattatt taactgctga cgaagaagat ttattcgtaa
     7021 gagctcaggc aaatgagcca ttaacagaag atggtaaatt cgtaaaccat agagttgttt
     7081 gtagaactgt aaatggtgct gttgaaatgg ttcctgaaag tagagtagat tacatggata
     7141 tatctcctaa gcaggttgta tctgttgcca ctgctatgat acctttctta gaaaatgatg
     7201 atgccaaccg tgcccttatg ggagcgaaca tgcagcgtca ggcagtgcct ctagttagaa
     7261 gagaagcacc aattatagga actggtatag aatacagagc agcaaaagac tctggagcag
     7321 ttgttgttgc tagaaattct ggtatagcag aaagagtaac agcagatgaa ataattataa
     7381 aaagagaaga tggaaacaga gatagatata atctacttaa gtttaaacgt tcaaactcag
     7441 gtacttgtat aaatcaaaca cctataataa ataagggtga ccagataata aaaggtgatg
     7501 ttatagcaga tggtccagca actgatttag gagaagttgc acttggaaga aactgtctta
     7561 tagcattcat gacttgggaa ggttataact acgaggatgc tatattaata aatgaaagat
     7621 tagttaaaga agatagatta tcaacaattc atatagaaga atatgaatgt gaagctagag
     7681 atacaaaact agggccagag gaaataacta gagatatacc taatgttgga gatagtgcaa
     7741 ttaagaactt agatgacaga ggtataataa gaataggtgc agaagtagac tcaggtgata
     7801 tattagttgg taaagtaact ccaaaaggag aaactgaact tactgctgag gaaagattac
     7861 ttcgtgcaat atttggagaa aaggctagag aagttagaga tacttcactt aaagtgcctc
     7921 atggtgaatc tggtataata gttgatgtaa aagtatttac tagagaaaat ggagatgatt
     7981 tatctccagg agtaaatgaa ttagtaagat gctatatagc taaaaagaga aaaataaaag
     8041 ttggagataa aatggctggt cgtcatggta ataaaggggt tatctcaaga gtattacctg
     8101 aagaagatat gccattcatg gaaaatggaa caccactaga cataatactt aacccacaag
     8161 gtataccatc acgtatgaac attggtcagg ttttagaagt tcatttagga cttgctgcta
     8221 agacattagg atggtatgta gcaacatcag tattcgatgg tgctaatgag tatgacataa
     8281 tggatgctct tgaagaagct gggtatccta gagatggtaa attaacttta tatgatggta
     8341 gaacaggtga gtcttttgac aacagaataa ctgttggata tatgtactac ttgaaactac
     8401 atcacttagt agatgaaaaa ctacatgcaa gaagtactgg accatactca ttagttactc
     8461 aacagccact aggtggtaaa gcacaatttg gtggacaaag atttggagag atggaggttt
     8521 gggctctgga agcatatgga gctgctcaca tacttcaaga aatacttact gttaagtctg
     8581 atgacgttgt aggtcgtgtt agaacatatg aagctatagt taaaggtgaa aatatacctg
     8641 aaccaggaat cccagaatca tttaaggttc ttataaaaga acttcaaagt ttatgcttag
     8701 atgtaaaagt attgacagat gaagaccaag aaatagaagt aagagaatct gtggatgaag
     8761 atgatacaat aggtgaattt gagctagatg ttgtaaatca tatgggagaa gttgaagaaa
     8821 gtaatataat agaagaaatt gaagatgatt ttgcagaaaa tgcagaagat gaggatatag
     8881 agaatttaga agaattcact gaagatgatt tatttgaaga agaaatagat tttgatagtg
     8941 atgactttga tatgtaaatt taattaatgt aaatctcaaa tagaagggag agaactcctt
     9001 gtttgaatta aacaatttcg agtcgataaa aatagcattg gcttctccag aaaaaataag
     9061 acaatggtct aggggagaag ttaaaaagcc agaaactata aattaccgta ctttaaaacc
     9121 agaaaaagat ggtcttttct gtgaaagaat atttggacca caaaaagact gggagtgcca
     9181 ctgtggtaaa tatagaagag ttagatataa aggtgtagtt tgtgatagat gtggagtaga
     9241 agtaactaaa tcaaaagtaa gaagagagag aatgggacat atagagctag ctgctcctat
     9301 gtctcacatc tggtacttca aaggtatacc aagtagaatg ggacttttac ttgatatgtc
     9361 accaagatcg ttagaaaaaa tattatactt tgcctcatat gtggtagttg atccaggaga
     9421 gactggatta aacgaaaaac aattgcttac agaaaaagaa tacagaactg ctcttgaaaa
     9481 gtatggatat acttttactg taggaatggg tgctgaagct gtaaaaacat tactacaaaa
     9541 tatagattta gagcaacaaa gtaaagacct aagagcggag ttaaaagata gtacagggca
     9601 aaagaaagtt agaacaataa gaagattaga agttgtagag gcatttaaaa agtctggaaa
     9661 taaaccagaa tggatgattt tagatgcaat accagtaata ccaccagatt taagaccaat
     9721 ggtacaactt gatggtggaa gatttgcgac ttcagaccta aatgatttat atagaagagt
     9781 tataaataga aacaacagac ttaaaagatt attagagctt ggagctccag atataattgt
     9841 aagaaatgaa aaaagaatgc ttcaagaagc tgttgatgca ttgatagata atggtagaag
     9901 aggtagacct gtaacaggac ctggaaatag accacttaaa tctttatcag atatgttaaa
     9961 aggtaagcaa ggtcgtttcc gtcaaaactt acttggtaag cgtgttgact actcaggacg
    10021 ttctgttata gttgttggac cagaacttaa attttatcaa tgtggacttc caaagaagat
    10081 ggcattagaa ttattcaagc catttgttat ggataaatta gttaaagaag gttatgcaca
    10141 taatataaaa agtgcgaaat ctatagtaga aaaagttaag ccagaagttt gggatgtttt
    10201 agaagatgtt ataaaaagtc atccagttct tcttaaccgt gcaccgactc tacatagatt
    10261 aggtatacaa gcatttgaac caatcttagt tgaaggtaag gctataaaac tacatcctct
    10321 tgtatgtaca gcttataatg cagactttga tggtgaccaa atggcagttc atgtaccttt
    10381 atcagtagaa gcacaagcag aagcaagatt cttaatgctt tctgtaaata atatacttgc
    10441 tcctaaagat ggttcaccta taactactcc atctcaggat atggttttag gttgctatta
    10501 tctaacaata gaagcccaag atggagctaa aggaacaggt atggtattta aagactttaa
    10561 tgaactatta cttgcttatt acaataaatc agttcatcta catgcattag tgaaacttaa
    10621 ggtaacactt gaagatggaa gaagttcatt ggttgaaagt actgttggta gatttatatt
    10681 taatgaaaat atacctcaag acttaggttt tgtagataga aaagaaaatc catttgcact
    10741 tgaagttgat ttcttagcag ataaaaaatc tcttggtaaa ataatagata aatgctttag
    10801 aaaacacgga aatacagaaa cagctgaatt actagattat ataaaagctc taggatttaa
    10861 atattctaca ttaggtggca taacagttgc tgttgatgat atgagtgtcc cagaagaaaa
    10921 gaaagtattt atagctgagg cagaagctaa agttgataag tatgaaaaag catacagaag
    10981 aggtctaatc tctgatgaag aaagatatga aaaagttata gagacatgga cagaaacaac
    11041 tgataaagtt actgatgctc ttatgggtgg actagataga ttaaataata tatatataat
    11101 ggcacattca ggagccagag gttctaaaaa ccaaattaga cagctggcag gtatgcgtgg
    11161 tcttatggca aatgcatctg gtaaaacagt tgagatacca gttaaatcta atttccgtga
    11221 aggtttatca gtactagaat actttacatc ttcacatggt gccagaaaag gtcttgccga
    11281 tacagctata cgtacagctg aatctggata tttaacaaga agacttgttg atgtaagtca
    11341 agatgttatt gtaagggaaa tagattgtgg aacagaagat actacagaga tttatgctat
    11401 aaaagaagga aatgaagtta tagaagagat atatgataga attgtaggaa gatatactat
    11461 agaccctata ttaaatcctg aaactggaga agttatagtt gaggctgatt caatgataca
    11521 agaagatgaa gcagaaacta tagtagcttt aggaattgaa aaaattagga taagaacagt
    11581 tcttaactgt aaaactaatc atggagtttg ttctaagtgt tatggtagaa acttggcaac
    11641 aggaaaagaa gttaatatag gtgaagcagt tggtataata gcagctcaat ctatcggtga
    11701 gccgggtact cagcttacaa tgcgtacatt ccatactgga ggagttgcag gagctgatat
    11761 aactcaaggt ctaccaaggg ttgaagaatt atttgaagct agaaaaccaa aaggattagc
    11821 tgtaataact gaagtatctg gtagagttga aattgatgaa acaggaaaga gaaaagaagt
    11881 aaatgtaata ccagaagaag gcgagactca aacatatgta attccatatg gttctagatt
    11941 aaaagttaag caaggtcaaa tgctagaggc tggagaccct ctaacacaag gatttataaa
    12001 tcctcatgat atagtaagag taaatggtgt taagggagtt caagaatata tagttaaaga
    12061 agttcaaaga gtgtatagac ttcaaggggt tgatgttaat gataagcata tagaagttat
    12121 agtaagacaa atgctatcta aagtaaaagt tgaagaccca ggagatacag atttattgcc
    12181 aggtggatat gaagatgtat taacattcaa tgaatgtaat aaagatgcta tagataaagg
    12241 tctaagacca gcagttgcta aaagagtttt acttggtata actaaagcat ctcttgcaac
    12301 tgattcattc ttatcagcag cttctttcca agaaacaaca agagtattaa cagaggcagc
    12361 aataaaaggt aaagaagacc acttaatagg acttaaagaa aatgttatat taggtaagtt
    12421 aataccagca ggaacaggaa tgaagaaata tagaaatata gctgttgaaa aaattgaaga
    12481 ttaatagcat atagagtaaa gaaatcttga caaccattat ttgtggtgtt aaaatagtaa
    12541 agtgtgttgt ttaaaagtac actaagggct tgaacaagtt cttagtgact tttagatggc
    12601 aataaatgag aactaaaaag catcaagcta gaaaagtgct aatatcacat atttatgtgt
    12661 tatagacatt aaattgtaag taacgaatgt tacaatcagt cagtatgaat attcatattg
    12721 atgtagttgg cggtcactga taagatcgaa aatttggaag gaggtgcaga tgatgccaac
    12781 aattaaccaa ttagttcgta aaagtagaaa agcgctagag aagaaatcta ctgctccagc
    12841 attacaaaaa ggttacaact ctttaaataa gaaagtaact gacgctagtg ccccacaaaa
    12901 aagaggggtt tgtacttcag taaaaacagt tactcctaag aagcctaact cagctttaag
    12961 aaaagttgcc agagttagat taactaatgg tatagaagtt tctgcttata taccaggaga
    13021 aggacacaac ttacaagagc atagtgttgt tcttataaga ggaggaagag taaaagacct
    13081 tccaggggtt agataccata tactaagagg tactctagat acagcaggtg ttgataagag
    13141 aagacaatca agatctaaat acggtgcgaa gagacctaaa gaagctaaaa agtaaaaaca
    13201 attaatgatt gattatagtt cggtgcttta aatatatata tatttacggc atcacggcat
    13261 taaatgattt atgtcgagta cctatgattt aagttaaata attaaggagg gaagcgaaat
    13321 gccaagaaaa ggtaatattc caaaaagaga agttttacca gatcctatgt atgggagtaa
    13381 ggtagttact aaattaataa ataatctaat ggtagacgga aaaaaaggaa agtctcaaag
    13441 aatagtttat gatgctttcg caattgttgc tgaaaagaca ggagaagaag ctttagaagt
    13501 atttaataaa gcaatggata atataatgcc tgttttagaa gtaaaagcaa gaagagttgg
    13561 tggggcaaac taccaagtgc caatcgaagt tagacctgaa agaagacaaa cattaggttt
    13621 aagatggcta gtaaaatata ctagagctcg tggagaaaaa ggaatggttg aaaagctagc
    13681 taaagagata atggacgcag ctaacaacac tggagcttca gttaagaaaa aagaagatac
    13741 tcataaaatg gcagaagcta ataaagcatt tgctcattac agatggtagg atatatagat
    13801 atcttataaa attgaggata tcataaggag gataaccatg gctagaaagt ttcctttgga
    13861 aagaactagg aatataggaa tcatggctca tatagatgca ggaaaaacta ctactacaga
    13921 aagaatcctg ttttatactg ggcaaactca taaaatagga gaaactcatg aaggagcttc
    13981 tcaaatggac tggatggagc aagaaaagga aagaggtata acaataactt ctgccgctac
    14041 tactgcttca tggaaagacc atagaataaa cataatagat actcctggac acgtggactt
    14101 cacagttgag gttgaaagat ctctaagagt tcttgacggt tcagttgctg tattctgtgc
    14161 aaaaggtggg gttgagcctc aatcagaaaa tgtatggaga caagctgaaa cttatggagt
    14221 acctagaata gcttttgtaa ataaaatgga tatattaggt gcagatttct acaatgttgt
    14281 aagcatgatg aaatctagat taaattctaa tgcagttcct atgcaattac caataggtaa
    14341 agaagattca tttataggta taatagactt attaaaaatg gatgctgtta tatacaaaga
    14401 cgacctaggg gttgaaatgg aagaaacaga tataccagaa gatatgaaag aattagctgc
    14461 tgagtggaga gaaaaattag ttgaatcagt agctgaaact gatgaagaat taatgatgaa
    14521 ataccttgaa ggtgaagaac ttactataga tgaattaaaa gttgctataa gaaaagctac
    14581 tatagcttgt gaaatgaacc cagttttctg tggtacagct tatagaaaca aaggtgttca
    14641 gttagtaata gatgcagttt tagattactt accagcgcca actgatatac cagctataaa
    14701 aggtatatta gcagatggag aagaagctga aagacattct tctgatgaag agccattctc
    14761 tgcattagca ttcaaaataa tgactgaccc attcgttgga aaactagcgt tcttcagagt
    14821 ttattctgga acattagaat caggttctta tgttttaaat gcaactaaga ataagagaga
    14881 aagaattggt cgtatattac aaatgcatgc taatacaaga gaagaaataa ctaaagtgta
    14941 tgctggagat atagcagcag cagtaggatt aaaagatact actactggag acacattatg
    15001 tgaccctgct aatccaataa tacttgaatc tatggaattc cctgagccag ttatatctgt
    15061 tgctatagaa ccaagttcta aagcagctca agaaaaaatg ggtatagctc ttcaaaaatt
    15121 agctgaagaa gacccaactt tcacagttaa gactgaccaa gaaacaggac aaactataat
    15181 atctggtatg ggtgaattac acttagagat aatagtagat agattattaa gagaatttaa
    15241 agtagaagct aaagttggtg caccacaagt tgcatataga gagactataa ctcaaccagt
    15301 tgatgttgaa tacaagtact ctaagcaatc aggtggtaga ggacaatacg gtcacgttaa
    15361 gattagagtt gctcctcaag agccaggtga aggatacaaa ttcacaaaca aaactgttgg
    15421 tggatcagta ccaaaagaat atgttggtcc agttgacatg ggtatacaag gagctatgca
    15481 atctggtata gttgctggat atccagttgt agacgttgct gttgagttat atgatggttc
    15541 ttaccatgag gttgactctt ctgagatggc gtttaagatg gctggttcaa tggcattcaa
    15601 agatgctatg aagaaaggta atgcagttct tttagagcct tacttcaaag tagaagttgt
    15661 tactccagaa gattacatgg gagatgttat gggtgactta aactcaagaa gaggattaat
    15721 ccaaggtatg gaagctagaa gtggtgcaca agttataaat gcattcgttc cactttcaga
    15781 aatgtttgga tactcaacag acctaagatc ttcaactcaa ggtcgtgcaa cttacacaat
    15841 gatattcgat cactatgagc aagttccagc ttcagttgct aagaaaatag ctgaaggaaa
    15901 ataatttaaa aataattaat aatataaata taaaaaataa tcagctagat gagagatatc
    15961 tctcatctag cacaagat
//
LOCUS       DAYAOX010000087        15814 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.087, whole genome shotgun sequence.
ACCESSION   DAYAOX010000087 DAYAOX010000000
VERSION     DAYAOX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.087"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            196..1386
                     /locus_tag="ACGI57_002062"
     CDS             196..1386
                     /locus_tag="ACGI57_002062"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFG9717139.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1556..2518
                     /locus_tag="ACGI57_002063"
     CDS             1556..2518
                     /locus_tag="ACGI57_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9717140.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2640..3302)
                     /gene="zmp1"
                     /locus_tag="ACGI57_002064"
     CDS             complement(2640..3302)
                     /gene="zmp1"
                     /locus_tag="ACGI57_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HFG9717141.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3402..3487)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3673..6591)
                     /locus_tag="ACGI57_002065"
     CDS             complement(3673..6591)
                     /locus_tag="ACGI57_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HFG9717142.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     ENENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNLGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(6709..6794)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6921..8099)
                     /locus_tag="ACGI57_002066"
     CDS             complement(6921..8099)
                     /locus_tag="ACGI57_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFG9717143.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            8474..8659
                     /locus_tag="ACGI57_002067"
     CDS             8474..8659
                     /locus_tag="ACGI57_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFG9717144.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(8724..11498)
                     /locus_tag="ACGI57_002068"
     CDS             complement(8724..11498)
                     /locus_tag="ACGI57_002068"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFG9717145.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(11670..12926)
                     /gene="glyA"
                     /locus_tag="ACGI57_002069"
     CDS             complement(11670..12926)
                     /gene="glyA"
                     /locus_tag="ACGI57_002069"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFG9717146.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(12998..14500)
                     /locus_tag="ACGI57_002070"
     CDS             complement(12998..14500)
                     /locus_tag="ACGI57_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFG9717147.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(14591..>15814)
                     /locus_tag="ACGI57_002071"
     CDS             complement(14591..>15814)
                     /locus_tag="ACGI57_002071"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9717148.1"
                     /translation="NKKIKFWRGVSMHELILISEKYKEDILDFFTDIHMHPELSFKEF
                     RTTKAIKDLLVSLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKY
                     KSRYDGIMHACGHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVER
                     GVMENPKVDAIFGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTID
                     PIVASSAIIMGIQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNE
                     VRKKISENLENIVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAI
                     VDPIPSTGGEDFSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVL
                     CQSVINYMDKLKNKI"
BASE COUNT         4968 a         2737 c         1929 g         6180 t
ORIGIN      
        1 tctttaaaaa actattatat aaaataatca tgataaaatt agaaattgga atttttaata
       61 tgtactgtac ataaatatat tttatataaa agtttatatt ttcaaacaag taatttttcg
      121 aaaattgtct aaattcagta aaaatatggt aatatataca taagcaatat aaatctataa
      181 aaaggagtgt tacatatgat atctaataaa atgcaaactt tagttgcaaa tagttctgtt
      241 ataagagcta tgtttgaaga aggaaagaaa ctttctgata tttatggtga ggaaaatgtt
      301 tttgatttta gtataggaaa tccaagtgtt gaacctccag agactattaa agcagttata
      361 aatgatattt taaatgaaga atctccaaat ttagttcatg gatatatgaa caactcagga
      421 tatgaggatg taagagatgc tattgctgaa cacataaata aaaaagatgg attaaatctt
      481 acaagagaaa atttaattat gacttgtggt gctgctggtg gtctaaatat tattttaaaa
      541 actcttttaa atcctggtga tgaagttatt gcttttgcac cgtattttgg tgagtataaa
      601 aattatacag aaaactatga tggaaaatta atagaagttc ctacaaatat agagactttt
      661 gaaccagatt tagatgctct aaaaaatgct attactccaa aaacaagagc tttaataata
      721 aacactccaa ataatcctac tggtgtcata tattctgaag aattattaaa aaatcttgga
      781 gaattattgg attcaaaaca aaaagaattt aatacaagca tatacctaat ttctgatgag
      841 ccatatagag aaataattta tgatggtgcc aaagttcctt gtgtactaaa atactataga
      901 aattcattta taggatactc ttatagtaaa tcattatctc tacctggtga acgtatagga
      961 tatattgttg ctaatgggca aatggatgat tttgatgatg ttatgtcttc attaaatgtt
     1021 gcaaatagaa tacttggatt tgtaaatgct ccttctcttt ttcaaagggt tattgcaaga
     1081 agtttagatg cagaagttga tgtaaatatt tataaaaaga attttgacct tctatataat
     1141 agtttaatcg atatgggata ttcttgtgtt aaacctaatg gtacattcta tttgttccca
     1201 aaagctccaa ttgaagatga taaaaaattc tgcaatgatg caaaacaatt taatttatta
     1261 ttagtaccag gttcttcttt tggttgtcca ggtcatttta gagtatcata ttgtgtatct
     1321 tatgataaag ttaaaagttc tttaccagca tttgaaaaat tagccaaatt atataattta
     1381 aaataactaa atcaaagtaa ttcaaataat taactacctt agtatagaat acttacattc
     1441 tatattaagg tagtttataa ttttatgtac tatattttat acataccata ttattccaat
     1501 atgcttgaat attcttatct atagtactaa tataaattac aaggaggtgt ttgacttgaa
     1561 aaaaggttat gtttatataa tactgactac cttatttttt agttcaatgg aaattagtct
     1621 aaaaactgtc actaatgact ttaatccctt acaaataact ttatcaagat tttttgttgg
     1681 cggtttagtc ttactacctt ttgctgtaaa aagattaaga gctcttagtc tttctattac
     1741 aaaatcagac ttaaaatact ttgcattttt aggttttatg tgtgtagtag taagtatgtc
     1801 attgtatcaa cttgctgttc taaacacaaa ggcatctgta gttgctgttt tgttcagttg
     1861 taatccagtc tttgtaatgc ttttagctta cttaattttg aaggaaaaaa tatataggca
     1921 caatgtaatt tcacttatat tagaagttct tggaattata gttataataa atccattgca
     1981 tacaaaacta actcttagtg gaatattctt aacattatct tctgctatta ttttttcaat
     2041 gtatactgta tttggtaaaa gaaaaacctt aaaatttggt ggaatagttg taacctgctt
     2101 tagctttata tttggtagtt tggaaatgtt gatattggtt ttactcacta aaattaattt
     2161 tatcgcagat atattaaata aaaataagct tcatgtattt gcaaatatcc ctatttttag
     2221 tggatatacc ttacacaata tgcccattat gatttatgta ttcgtatttg ttacaggtgt
     2281 tggttatgcg ctttacttta tggcaatgga agctacatct actagtctta catctttagt
     2341 attcttcttt aaaccagtaa tatctccaat actagcatta ttaatactta aagaaattat
     2401 ccctataaac atggttatag gaattttatt gatagtaatt ggttcaataa tttctattat
     2461 acctacgata attactcaaa tgcataataa acacgttgaa gccttgtata aggagtagta
     2521 attgataatc tatcatagtt atacataaga caaaatttat tagatttaaa cgcaatataa
     2581 aagagatgct attataatag catctcttta tttatattta ttttaaattt attaaaactc
     2641 tatttagcta aattttgcaa aaagctataa gtttgtggac aagctgattt taacttacta
     2701 tttgtatctt gatttaaata gtaatatgca aatgattctg caaaaaattc ttctggatat
     2761 acacctaagt agtttacatt acctaaagaa cgaccttctt tagcaaatat ttgtttgaat
     2821 tgtgctgatt tagatatatc atttaatact atatggtcta ttgcatgtgc tgtttcatgt
     2881 aattctaaat ttattgcatc atgcccttta cctttgttac taaatcctat tctaagagct
     2941 actgtacttc cacctaaacc tggaacatca tcccaagttt ttccagtacc ttcccatccc
     3001 ttaggaacta caccttttaa ataagcatat tctttttcat ctgttaactt tccacttaat
     3061 aattttattt ttaagttatt ttgctttaat gcatttaaat actttccatc tatattagct
     3121 aatctattta ccatagcatt agcttcgttt ttatcgtaat ttccagttgg aaaaactact
     3181 atttgactaa gtgtgtcttt attttgttgt atagtagtac tatctgcatg tgcacttact
     3241 ggaactgaac ttattaagaa tattgatatt atagctatta ataatttttt acttggtctc
     3301 atattattcc ctcctttgag ttaggccggg agtaataaat ttcaaaataa aaaagatacc
     3361 caagagtatc tttgtatata caaaaagaaa tttttactct tttcggcaac tggctggcat
     3421 ggtttactaa actttagccc ctatagcttt gcgtgactag gtttccctag ttttgcctca
     3481 tttaatttta tatctatatt atagcttata tctttttaat tgtctatatc cctttggaac
     3541 ttttttctaa aaagttatta tttttttaca taaatcttca aaaaaaaata tcttctaaca
     3601 atattttcat tattaaatat tgttttagaa gatatttttt aatataactt atatttttta
     3661 taatgttagg gtctaatttg tatttttatt tcttcttaat acgataagtc ctacaatagc
     3721 tcctataacc aaaagtattt ctccaataat tgtagtacta tcccctgttt ttggtggtac
     3781 tggtggattt accaaagtat catctttaac tttattattt ttttcttctg acactgatgg
     3841 ctttactact tctggtattg gaataatctg tttaacagga gtttctatca tctcttctga
     3901 tggtggtact ggcggattca atacctctgg tattggcgga tttattatat cttctgatgg
     3961 tggtattggt ggatttataa ctatagtatc ctctgtatca gatgaacttg atggtttcct
     4021 tggtttatct gtgcttggtg gtactggtgg atttactatt ggttcatctg tatttggtgg
     4081 tgctggtgga tttactggtg gattttcttg tcttatgttt tctatatttc caatatccac
     4141 tattctacca atatctttat taccaatttc aaccttaatt ttatccttca gtttttcata
     4201 tccacttgga gccattatct ccactaaata atatgttcct ggttttttta tactagattg
     4261 aattatacca tcttcatcac taactaaatc cttaactact atatttccaa atgcatcttc
     4321 taaattgaac ttagcacctg aaagtacatt tttatctaaa gcatccactt ttttagcttc
     4381 aaaagcataa ccttgcttag tagtctctac tcctggagga cttgaaccat taatctgagc
     4441 cacatttcgt atagtctccc caacttttac atttgtgaag tctgcatcaa atgtaaccca
     4501 actttgcaca ggttcccctt cttttatctg tttaaattcg tatttattta ctaatcttat
     4561 tgtatttttt ccatctccag cttctttttt aagttcaaga ttatcagaat attttatatt
     4621 agatatttct agagctgagt ttacttcatc taaaagattt acttgtcctg catcataaac
     4681 cccataagtt gacatattga ttttgtattt aacaatctgg ttatcactac tatttaaaac
     4741 ttttttatca acttcttttt ttgcgcttat taatccagaa tttttcttaa agtttatctc
     4801 atcattttta tcaatttcac tattatttgc atcaagaaca gctctattta tatagctatc
     4861 ttcaatacta attaccttta atgtataagc tataaaaaac tttgattcca cttcttcttt
     4921 actgagatta attgtaaatc cagttataac tttatctact tgcccacttt catttctttt
     4981 ttcttctact ttatatgaaa tattattggc aattttttct ttactccaag gagtccagct
     5041 cccattcatt ggaatttcta caactctaaa atcatgccca gcttctcctg aagtagttaa
     5101 tctcatgcct tctggtatat tatctgttat aacaacatta cttattggtg atttatcatc
     5161 atttattgaa atctgatagt ttattacttg accaacttga gtaatatttg ttgctcctga
     5221 atgtccttta gtaagaagat aatcatctcc tctatcaaat atacttacag tagcatcatc
     5281 ttcttgtatc ttcttaccat cagattcaat cctagctaca ttcttatatt tattaccatg
     5341 ttttgcattt ttgtcactga ttctagcctt atacttaaca gtatacgatt tatctatatt
     5401 tccaagatta actgaaatag aatttttact ctgttcaata ttaaaatcac taacttcttt
     5461 tccatcactt gttactacac ttacagaacc atttatcaat gtcaatcctt caggaagctc
     5521 atcatacaaa ataatatctt ttaactcttc ctgcttttca tttacttgta tactgtaatt
     5581 tatatcttta tttttaactg gcataaatac agtagttgat ttttttcctt caacaagctt
     5641 tttaagtgtt cctttagcat ctggaacatc cgttttatct ccaggtttta tttctacatc
     5701 tatatctcca cttccacttt ctatttttcc agtagtttca atatgtattt ttgcattagt
     5761 atcttcatca acctttttaa catatgcttc caaacctatt ttaccttgca ttacactttg
     5821 gccattaggt aattctctaa atgtaagtgt atactctcca ttactttctg ttttatcaaa
     5881 gcattcactt gtataatcta aattaacttt attgaataca tctggcacct taaaagttat
     5941 tgaatctcct gcttgtattc cttttgatgt tggagtataa gttaaattaa atgatatttt
     6001 tttcattgat tccgtttcta ttgtattgtt attaattttt acaacaaatc catagtcttc
     6061 attttcttca ttaagttctt cttctaaaac tttcaataaa ttttcatttt ctaatagatt
     6121 atcttcctct ggagtttttt ctgtattttt attgtctagt ggtttttcat cttctaaagc
     6181 ttgttctgac tttttattat ctgttgactt atcttcttct ggtatctgct ctggcttttt
     6241 attatctatt ggcttttctc cttctggagt ttgctctggc tttttattat ctgttgactt
     6301 atcttcttct ggggtctgtt ctggtttttt attatctgtt ggtttttctt cctctggggt
     6361 ctgttctggt tttttattat ctgttgcctt atctttctct ggagtctgtt ctggcttttt
     6421 attgtcgctt tccttctcct ctccagttga tgaattagtt gaaccacttt gaatctgact
     6481 attctctcct aattccgaag cataagatac agttggatat attgtagaca ccaccatact
     6541 taaaatcata attaaactaa ttaataatgc ctttctattt cctttcttca tgtaaatctc
     6601 acccctttag gttacctata atttataatc taatcttcca ttaatatcgt atataaagta
     6661 atttttacta aaaaattata caaaaaaagc tatgaaaata aataaattta tattcatagc
     6721 cggttgcacc actgactcag tatatccata aggtgcccaa taatagaata tacttcaaca
     6781 tctctataat atttcataaa aacaattaaa attgctttat ttatattaaa aatacaatta
     6841 tatacttcta ttatattatg cctctatact attgtctatc ctttttacat tttttacctt
     6901 atgatgagat taattaataa ttaaataaaa tccttatcta caacaattac acaattatat
     6961 tcaggattta tctccttaac taattttgtt atatcttctt ttaagtcatc ttctgacatg
     7021 tttttggaaa attcggaagg attaactact atatcaaata ttaaattctt cttgcttcct
     7081 tttccaacta ttctaaaatc atgcatagat tttacaagac tatttttctt aagtatttct
     7141 tcaagttctt ttttggctga cataacttct ttatcttcaa gacatattgg gtccatgtga
     7201 attactagat atatatctaa tttttcagat atttctctct ctgcagtatc tataatttca
     7261 tgtatttcca ttatatctat gttggatgga atttctgcat gtatagatgc aatacatcta
     7321 ccaactccat agttatgaat aattaaatca tgtgtaccta ttatatattt ataagataac
     7381 aatagctcta taatagaatt taccatttct tcatccggtg cttcccctaa aagaggattt
     7441 atagtatctt ttacaagaga gaaaccagca taaagtatca ctaatgatac tacgatacca
     7501 acatatccat ctatgggaaa attagtaaat ctagctacaa taaatgatat aactacacaa
     7561 cttgaagtaa atacatctcc taaagcatca agtgaaactg cttttaaggc tgaagaatca
     7621 attttatttc ccataaattt attaaaacga ctcaaccaga ttttaatcat tatagatgca
     7681 attaaaagta taagtggtat tacttcaaat tttacaggaa ttggatttac tatcctttct
     7741 atagatgact ttacaaattg aagtccaacc aacatcacca taaatgctac tattaatgct
     7801 gataaatact ctattcttcc atgaccaaaa gggtgttctt tatcagctgg cttacttgct
     7861 aatttaaaac ctatcatagt tattgctgaa gatgccatat cagaaagatt attaaatgca
     7921 tcagccatta tagctatact agatgtgagc attcctatga atacctttat cacaaataaa
     7981 agtaaattgg ataatatacc tactacacca gctacataac catacttatt tctaacatct
     8041 gtattttgaa cgttttcgct atctctgata aatgttttga ctaggatttt ggaaaacatg
     8101 aaacttacct ccctgccctt tattttatta acaaatttta taatctatat ctttatttat
     8161 gataacataa tttatatata aaattcttaa aaatcactct acttcagtaa aatattattc
     8221 tatatatcta tttagatacg aattttatat ttagtaaagt ttttatggct tagtgtctgt
     8281 ttaatagatt ctatataagt ttttacaaag attatttatt atgataatat catttttccc
     8341 aagtattact tagaatatga tttttttgtt ttgtctgtac atattttaaa tttatactat
     8401 atttatctat attttataac cttatgatac actataacta taagatttta aatggaaagg
     8461 aatgaaataa atcatgtttt ttatcagtga tatttacaca aaatcaccta ttaaatttga
     8521 tactcctttg caaaaagagg catataaaat attacaaaaa ttagatattg actttgaatg
     8581 tgttgacaca gatgaagcaa ttacaatgga agattgtgtt cagattaata aaaaattaaa
     8641 tatgaaaatg gtaatataaa acaacattaa acaaacaaaa accgcaaagt tacaatatat
     8701 agtactttac ggtttcataa gaattaattt ttactattaa agttcatagt tctactattt
     8761 ttcattgttc taagaattag ctttacaaat tcatttgaca ataggatagt ggatgcaaat
     8821 agtattatct ttatccacat atcaaagtcc aaagaaacag aattaaagaa actctggaac
     8881 acttgtgtaa atattatttg aattatccct gtaactacta ttatttgcaa tgccagttta
     8941 tttttaaaga aatttggtat tgtactattt aaaccaaact ctctacaatt taatgcatta
     9001 aaaagggcac tgaatgcaaa taaagaaaat ataacagtac cttgttcttc tgatgtagct
     9061 cccaatatat taaaagctga ttgagtaaat acaattgtaa ttataagaat tgcatttata
     9121 ataatattta caaacataga cctagctatt attccagaat gtctatttat tggttttctt
     9181 ttcaatacat aatctctaac aggttctaat cctaatgcta gggctggagg accatccatt
     9241 attatattta cccataataa ttgtattgta gtaaatggca tatccttacc agtaagttga
     9301 gatattatag caattataaa tgctactata tttacagtaa gctgaaactg tataaatctt
     9361 tgaaagttct catatatacc tcttccccat tttattccct ctactatagt actaaagcta
     9421 tcatcagtca atattatatc tgctgcattt ttagatactt ctgttcctga aatacccatt
     9481 gcaattccaa catctgcttg actaagtgct ggggcatcat ttataccatc acctgttaca
     9541 gctacaactt cgccactttt ttgaagtgct gaaactattc tcatttttgt atctggttta
     9601 cttcttgcaa caatagatat tcctttgatt tcttctctca attctttatc tgtcaataca
     9661 tctatatatg ttgcttcaac tgcctttttc ccatcattta ataatcctaa ttcatttcca
     9721 atagctgttg ctgtatttat attatctcct gtaagcattt ttacatctac tcctgcatta
     9781 aatgctttat caatggaatc tttgacccct tctctcaatg ggtccactat tcctacaaat
     9841 ccactaaaaa ctaaattatc ttccttcata taacttcttg aatttcctat taaattcaat
     9901 tcaccttctg aagtcattgc aacttctgtt ttagaattac tcattttttt ataagcaaat
     9961 cctaatgttc tcatagattt aatttgtaac ttcttaatct catctaaaat acttttttct
    10021 acttttggag ttattggaac aatatttttt ccttgttgta cataagaaca tttttttaat
    10081 aatacttctg gagcaccttt tgaaagtaat atatctgaat cctcttgacg aattaatgtt
    10141 gacatcttct tttcttctga actaaatggt atttgtgata tcaaatacgt ttgttttctc
    10201 atttcattat agttcttatc attatgatat aaaagtaatg cacactctgt tgcgcttcct
    10261 atatatttaa atgaattatc ttctttttct atatctgcag ttgaatttac tatacagttt
    10321 tgttcaaaat agctattact ttgatactct cctccactta tatatttacc atcaacatat
    10381 gctacctcta ccatcatttt gttttgtgtt aaagtccctg ttttgtcgga acatattaca
    10441 gaaacagaac ctatagtttc acaggcttct ttctttgtta ctaatgcatt tatctttgcc
    10501 atcttttgca ttgttatagc taaggtaata tttatcatag ttggcaaacc ttctggaaca
    10561 gttgcaacta ttaatgcaat acaaaccata tatgcatttt tagcaggttc taatgattgt
    10621 aagaaagata atatactgct tgtatctaaa attaacatac cttttctctg catctgtata
    10681 atcataaaca tacacaataa agtagctatt atgcctgata ttcctgctat tttagcaccc
    10741 aacttcccta atttaatttg aagtggagtt tgctggtctt catttgataa attctgtgct
    10801 atcttaccca tctctgtctt atcaccagta tgagtgacaa ccattatgcc cctaccatat
    10861 gctacaagag tccctccaaa taccatattg acttgctttg caggtattgc atcctgttcg
    10921 attatttctg ttttagaata aactacttcc atatctaata cagcatcagc attcttagct
    10981 acatcatcag attctcctgt tagcatatct tctctaagtt tcaaatttat agactgtata
    11041 agtcttccat ctgctggtat catatcccca gtttctatat atacaatatc tcctggtact
    11101 aaatcatttt ttgatatttt aattatcttc ccatttctaa gcacctttac ttctatattt
    11161 tctgttaatt ttgacagggc atgagctgct tttttagatt ttccttcagt tattatgcct
    11221 atagaaattc caataaaaat agcccctaaa atacctaatg cgtcatgtaa ttctcccaca
    11281 aaagaactta ttactgctgc tcctatgaga attaatatca taggttctgt caaactttct
    11341 cccaactcgt cccaaaatgt tctaccttct tttatagtaa attcatttaa tccatatcta
    11401 agcttccttt cttctacctc attatcatca agtccaattt ctggatttgt ttttaagtat
    11461 tttaatactt ctttggtagg tttattataa tacctcataa ctttatcccc cttgttaatt
    11521 atctataagg aattatatgc ttatgatagc ttgtatatta cttttcaaaa aaaataaggt
    11581 gttgattaat caacacctta tagtggttat atattattaa attttatgtt acaggcttta
    11641 taaattgtga gctatacaaa tcaaaatatt taatcttcaa aatacccttc tggatataat
    11701 ggaaacttag atataagttc ttgtgcctct gctttacatt catctaatac ttctttatta
    11761 tcaatatttt ctgctacctt attcattatc cctgctatct gtataacttc tttttctttc
    11821 agacctcttt gagatattga tgtaagacct atacgaacac cactagttac aaatggtgat
    11881 tgtgggtcgt caggtataac atttttatta actgttattc ctacatattc aagagctttt
    11941 tcaaattgtt ttcctgtaag attcttactt cttaagtcaa ccattactat atgattatca
    12001 gtacctccac ttacaatact aaaaccatgt gaaatcagtt cagaagctag agtctgagca
    12061 tttactacaa cttgctccat tatcttttta aatttatctg tacttaaata taatagtgaa
    12121 aaggcttttg ctgcaactgt attaagatgt atagaaccta atgttcctgg aaaaacacct
    12181 ttatcgagtt tctttgcatg ttctgcttta caaagtacaa taccacttct tggtccacag
    12241 aaagtctttg tcgtagatga agaaacaaaa tctgcataag gtacaggact aggtattact
    12301 ttcgctgcga ctaatcctgc aacatgtgcc atatctacca taaagtaagc tcctacttct
    12361 tttgcaacct ttgatattct ttcataatct attaatcttg gatatgaact tgcccctgca
    12421 attataagtt ttggtctaca ttcttttgcc tttgcttcaa gagcatcata atctatacgt
    12481 ccagtatttg ggtctacccc ataaaattca tatttataaa tgtcatgtaa aaaattaact
    12541 gcgctaccat gtgtcaagtg tccaccttgg tcaagacgca tacttaaaac agtgtcattt
    12601 ggttttaata ttgatgaata tacacaatag ttagctgtag aacctgaata cggttgtacg
    12661 tttacatgtt ctgctccaaa tacttctttt gcacgtttta ttgctagggt ttcaagttta
    12721 tctgcttctt cagaacctgc ttgaaaacga gcccctggat agccttcttc tgttttattt
    12781 gtaaatacac atccacttaa ttcaagtact tctgttggtg ctgtactttc agaagctatc
    12841 atctcaatat tgtgttcttg tctaaccaac tcatcagcta caatcttgta taactctggg
    12901 tctgaaattt ttgttgtttt taacataata atcctcctct taattaaaga atatatttta
    12961 ttagtttaat cttcattgat aaatttatta taacggtcta taaaacgtaa gatattcctc
    13021 caccaggtcc taatggtatg ccaataccca tgaatagtaa caatattatt atttctatta
    13081 atgtaaatac cactgagtat ggaatcatat tcgatatgac agtacctata cctatatctt
    13141 tatcatatct tctggcaaag gccaatataa ctggaaaata cggaaaaagt ggacttattg
    13201 gatttgttga agcatctcca atacgataag ctatttgagt taatgctgga ttaaagtcta
    13261 gtagcataaa catgggaaca aatataggtg ctaaaatcgc ccattttgca gaagcacttc
    13321 ctacaaataa attgataaat ccagatagca atataaatcc tattatcaat ccatagctag
    13381 gtattcccga ctctgctagt aattttgctc ctgttattga taaaattgtt cctaagttac
    13441 taagattaaa taaattaata aactgggatg ctataaatgc taggactata tatccaccca
    13501 tatcactcat actactaccc atcatagata ctaaatcttt gtcacttttt atcttcttac
    13561 ttacttttcc atacactaat cctggagtta aaaatataat agtgattata ggaactatac
    13621 ctttcattaa aggtgcatta ctagatagta tagaaccagt ctctggatct ttcataaatg
    13681 cattgtcacc tatacataag gcaactatca ctactactac aactaacaat gataataaag
    13741 cgtattttat acccttattt tcaagttcac tcaattcctg tatatcctta tcatttgttg
    13801 aatcattata ttttgtaaaa cgaggagcta taaatttttc agttacaaat gctcctgcaa
    13861 aagttaaaac tatcgctgat ataaaagcaa aataaagatt cattgctgga ttgccttggt
    13921 aagatgggtc catcatttga gctgctggta ttgtaaatcc agctaacaat acatctgtag
    13981 tactaagcat aacatttgct gcaaatcccc ctgcaacacc tgcaaaagct gcaaacatac
    14041 ctacaagagg atgtcttcca attcctaaaa acacaagtgc tgcaagtggt ggtaatatta
    14101 taaatcctac atctgctgct gtatgtgaca acatacctgc aaatacgatt gctacagtta
    14161 caagtttttg tggaacttta cttataccct ttttcattaa cacttccata aatccagatt
    14221 tctcagcaac acctgctcca agcattgtaa ccagtactaa tcctaaagga gcaaatgact
    14281 gaaagtttgt aactatgcta cctaaatatg attgaatctg ctctttctct agtagattta
    14341 ctacctttac aacttctttt gtagaaggat gtatttcttc tactcctaaa ttagatataa
    14401 ctgctgacaa tattacaaca attatacata gtaataaaaa tatggttact gggtctggaa
    14461 gtttatttcc tgctctctct acaacattta gtgccctcat caataatcca ctatctttgt
    14521 ttttttcctg tgaattaatt ttcctcaaac taataccccc tcataaactt tatctccaga
    14581 atgctctatc ttaaatttta ttttttaatt tatccatata atttataaca gactgacata
    14641 gtacatttgt acctaccaat atagaatttt tatcagcttt aaatttagga ttatgccact
    14701 gatatataca atcttgttct ttgttgccta cccctaacca gtaaaaaaat cctggggctt
    14761 tttccatgaa tatagaaaaa tcttcccctc ctgtagaagg tattggatca actatagcat
    14821 cttctgaata caaatcacac acagacttac aagctattgc gtacatctct ttactattta
    14881 taacagctgg taaatcaaat aaataatcta gctttgcagt tgcttgatat ccacgagcta
    14941 tctcacaaac tatattttct aaattttctg atattttctt tcgtacttca ttactaaagc
    15001 ttctacatgt gcctgtcata tctacctttt ctgaaattac attatttgca gtccctccat
    15061 ttatagtacc tattgatata acagctgatt ctaatggact tatgtttctg cttactatgg
    15121 tttgaatacc cattataatt gcacttgatg ctactatagg gtctattgtt ctgttgggta
    15181 tacctccgtg acctccaaat ccatttactt ctatcttaat agtgtctact gctgccatta
    15241 acccacctaa cttaacacct atttttccac aaggtatatc tggatgatta tgaagtccaa
    15301 atatagcatc aacttttgga ttttccatta ctcctcgttc cactagcatc ttcgcacctt
    15361 tattgacttc ttcggctggt tgaaatataa actttacatt tccatgaaga ctatctctaa
    15421 tatgagacaa tacatatgca catcctacaa ggcaagacgt gtgtatatca tgcccacatg
    15481 catgcattat accatcatat ctggatttat acttaatatc aacctcttca tatattggca
    15541 atgcatcaat atctcctctt agtgctactg tgggaccatc atacttccct tttaataatc
    15601 caacaactcc tgtttccatt cctaaatcta atatttctat gtctaaactc actaataaat
    15661 cttttatagc ttttgtagtc ctaaattctt taaaacttaa ttcggggtgc atatgaatat
    15721 ctgtaaaaaa atctaaaata tcttctttat acttttctga gattaaaata agttcatgca
    15781 tactaacacc tctccaaaat tttatttttt tatt
//
LOCUS       DAYAOX010000088        15787 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.088, whole genome shotgun sequence.
ACCESSION   DAYAOX010000088 DAYAOX010000000
VERSION     DAYAOX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.088"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            207..1424
                     /gene="ilvA"
                     /locus_tag="ACGI57_002072"
     CDS             207..1424
                     /gene="ilvA"
                     /locus_tag="ACGI57_002072"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HFG9717149.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1519..1899
                     /locus_tag="ACGI57_002073"
     CDS             1519..1899
                     /locus_tag="ACGI57_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFG9717150.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2183..2665
                     /locus_tag="ACGI57_002074"
     CDS             2183..2665
                     /locus_tag="ACGI57_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFG9717151.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2686..3501
                     /locus_tag="ACGI57_002075"
     CDS             2686..3501
                     /locus_tag="ACGI57_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9717152.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3552..5102
                     /locus_tag="ACGI57_002076"
     CDS             3552..5102
                     /locus_tag="ACGI57_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9717153.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5199..6551
                     /locus_tag="ACGI57_002077"
     CDS             5199..6551
                     /locus_tag="ACGI57_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFG9717154.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6652..7440
                     /locus_tag="ACGI57_002078"
     CDS             6652..7440
                     /locus_tag="ACGI57_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9717155.1"
                     /translation="MIFLEEWGDAMNIINKIFWEEDRIGMITNNLEADTHSHCMLQLF
                     LGIEDSIEITVNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMN
                     DFGYWICDNDGLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITEL
                     LNLLRTCNCDNHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLS
                     GKNVTEASMTAGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7487..8206
                     /locus_tag="ACGI57_002079"
     CDS             7487..8206
                     /locus_tag="ACGI57_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HFG9717156.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8330..9730)
                     /locus_tag="ACGI57_002080"
     CDS             complement(8330..9730)
                     /locus_tag="ACGI57_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFG9717157.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9734..10624)
                     /locus_tag="ACGI57_002081"
     CDS             complement(9734..10624)
                     /locus_tag="ACGI57_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9717158.1"
                     /translation="MTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKATFYKHF
                     ETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRTTINTYI
                     RENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDALIHQFI
                     YIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYFSLHNKS
                     LFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAYLEKEDT
                     LRPKVLLLNSIKNQLKKETL"
     gene            10903..11550
                     /locus_tag="ACGI57_002082"
     CDS             10903..11550
                     /locus_tag="ACGI57_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9717159.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11875..14514
                     /locus_tag="ACGI57_002083"
     CDS             11875..14514
                     /locus_tag="ACGI57_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFG9717160.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14572..15675)
                     /locus_tag="ACGI57_002084"
     CDS             complement(14572..15675)
                     /locus_tag="ACGI57_002084"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFG9717161.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVREAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
BASE COUNT         5947 a         1901 c         2753 g         5186 t
ORIGIN      
        1 gtaaaaaata tttttaaatt atcatttaat gtaatttaaa tgaattgaag atatatgttt
       61 attgtatgca attgttgtat ttttaggttt tttgtgatta attaaaaaat tataaaaatc
      121 gtttacatgt tgatttttaa aaaaaaaaga tatagaatta tatagaaagt aataaacaaa
      181 aattggattt acgggagtgg aatgaaatga tgaatgttac cttaacagat gtgaaagagg
      241 ctagagagac cattaagaac atagtaaaaa gaactgactt acttgaaagt gttaaactta
      301 gcgaaaaaac aggagcaaat gtgttctata aatgtgagaa tcttcaaaaa acaggttctt
      361 ttaaattaag aggagcttgt aacaaaatag cgagtttaac agatgaagaa aaggcaaatg
      421 gagttatagc ttcaagtgca gggaatcatg ctcaaggagt tgcattggga gcaaaaatga
      481 ctggtataaa atctacaata gtaatgccag caactgcgcc tttagcaaaa gttagtgcaa
      541 ctaaaggtta tggagcagaa gtagttttaa atggtgcggt ttatgatgat gcatatgcaa
      601 aagctgttga aatacaaaaa gaaacaggag caactttctt acatccattc aatgataagt
      661 atgttatagc aggacaagga actatatctc ttgaaatttt cgaacaatta gacaataaag
      721 ttgatactat actatgccca gttggtggag gaggaatcat ctctggtgtt gctgttgcag
      781 caaaagcatt aaatcctaat gtaaaaatag tcggtgttca aactgcaaat ataccttcaa
      841 tgaaagagtc aataaaaaat ggaaaagtaa caacagcatt taatgatact acaatagctg
      901 atggtatagc tgttaaaact ccaggagatt taacttttga aataataaat gaattggttg
      961 atgaaatagt agtggttgaa gagacagaaa ttgcagaatc aatattattt atgatggaaa
     1021 gtcaaaaaat agtttcagaa ggtgctggtg ctgtatgtac agctgctata ttaagtggta
     1081 agtatgtacc agcaaaagat gaaaatgtag tttgtataat atctggtggt aatatagaca
     1141 taaatacgtt atatagaata ataggtgttg ctttagctaa agaaggaaga agatattcat
     1201 ttagtacaat tatggaagat aagccaggaa actttgctga gttaactaga ataataagtg
     1261 agaatggtgg aaacatttta agtgctaatc aaggtaaatt atcagcaggg gaagctctag
     1321 gaaaacaaag tgcggaattt atattagaga caatagatta tgaccatata gctagaataa
     1381 agaaagctat agaagaaaaa ggttttaaaa ttatagaatt ataataaaaa gtgtatataa
     1441 tatacaagaa aaatagtata atagtgtatg aagatacatt ttatgtgtat tagattaacg
     1501 aattaggagg attttaaaat gaaacatgaa gtaatacata ctaatgatgc accagcagct
     1561 ttaggaccat attctcaagc tataaaagct ggaaacttgt tatttgtatc tggacaagtt
     1621 cctcttgtac cagagactat ggaagtagtt gaaggagatg tacaagctca aactgctcaa
     1681 tctttaaaaa atcttaaagc tatattagct gaaagtggag ctgatttttc aaatgtagtt
     1741 aagactacag tatttataaa agatatgaat gaatttgggg ctataaacga agtttatgct
     1801 gaatattttg gagaaaataa accagcaaga gcttgtgtag aagttgctag attaccaaaa
     1861 gacgttaaag ttgaaataga agtaatagct gtattataaa aatacaatat ttttaatttg
     1921 aaaaggctga aaaactatat tctagtgatt cagccttttt ataaaaaaat aaatcacaat
     1981 aaagaacatg ataaatattg tcgaattaaa aaataaaatt aaaataaata ttgcaatatt
     2041 tttgagaagg ttttataatg agtatataat ttgaaacgtg taactattaa attaggcatg
     2101 agttcttgga tatttttatc ctgaggattc atgccttttt tgtattgaaa ttcaaaaatc
     2161 tatttaataa ggaggaagta ctgtgttaaa ttttttcaag aaaaataaaa gctataagtt
     2221 acatgcagta gtgagtggta acagcattaa tattgagaaa gtaaacgatt ctgtgttttc
     2281 aaaaaagtta atgggagatg gagttgcaat tattcctaat agtaatgttg ttgtagcccc
     2341 ttgtaatggt aaagtaactg tgcttacaga atcaaagcat gcctttggta tggtctcaga
     2401 tgagggtgtt gaaatattag tccacatagg aattgataca gttagtttac agggtgaggg
     2461 gtttaaaaat gaagtgtctc aaggagatac tgttaaaaaa ggtagtccaa taataagttt
     2521 tgaaagagaa aaaataaatt ctcagggaat tgattgtaca acaataataa ttgtattaaa
     2581 tcatagcgaa ttttcagaaa taaattgcat ggtagaaaat gaagttgttg cagggcaaga
     2641 tactgttatt gaaattatga aataatagga agggattaat tcaatatgag agtagtaaaa
     2701 atctttaata ataatgcatt atctacagta actaatgata ggaaagaagc tattttacta
     2761 ggtcttggta taggttttaa taaacgtcca ggagataaag ttaatgaaga taaaatagaa
     2821 aagatttatt atgttcaaga ccacatgcaa acaaaatttt tagaattatt aaaaaatgtt
     2881 agtccagaag tgatggatgc ttcacaacaa attataagtc ttataccagg aaatgaggga
     2941 aaatttaata ataaagggat attatcttta gtggaacata ttagttttgc aatagataga
     3001 atgaaaaata atgttttttt acctaattta atgcttttgg atataaagat gatgtattca
     3061 aaagagtttg aattaggagt aaaagcttta gaaattatat atagagtatg tcatatccat
     3121 ttgcctgaag atgaagcagg ttacatagct ttacattttg tcaatttaca atcaaatgat
     3181 aatttggcat atgatacttt aaaatttgta aaaggtagta ttgatttgat taaagaatgt
     3241 tatggattag aattagacga atcgagttta agtacattac gttttagaac tcatcttaaa
     3301 tttcttgctc aaagaatttt taagaatgaa atttgtcagg atgataaaat gattgaaatg
     3361 tatgattatt taattaacaa tcatcctaaa aataaagaat atttagaaaa gttaaatgta
     3421 tatattgaaa aggagtttag gtataaatta gataaacctg aaaaaattta tctactacta
     3481 cacctaacaa aaatcttata agaatgagat gaattagtaa tagaaatata taattttagg
     3541 gggggataag attgaaagaa aaactacttg gaggactgga aacatttgca aaggcaatgg
     3601 ttcagccatt aatgtattta tcgagtgcag gaatattaat ggtggtaggt gtactactta
     3661 caaatgatat gttattagga gtacttccat tttttaaatg ggagccaatt caaatatttg
     3721 gaagcctaat ttataactgt ataatggcta ttattaataa tcttgctgtt atatttgcta
     3781 taggtattcc tgctgcacta gcaaaaaaag agaagcacaa agcagcgtta ataggattta
     3841 tgagttatat aatatacctt acagcttcca acactatgtt aacatcacta ggtcaactag
     3901 caaaagaaag caagatgctt ggactgatag ggactggaca agctactatt ttaggtattc
     3961 aagttgtaga tacaagtgta tttggtggaa ttattttagg gtgtatagct ggatatgtat
     4021 ttaatcgtac aagcaacaaa acatttaaag gagctttaca gatatactcg ggagcaaatt
     4081 ttagttttat atgcatgttc tttgtatcca ttttaattgg agttgtgagt aactttatat
     4141 ggccattagc tcaacaagga atagcttctc ttgcaaatgt catcaaagat acaggtacat
     4201 ttggtttatt cttatatggt tttttagaga gaatattagt accaactgga cttcatcatt
     4261 taatctatac accatttcag ttttctgatt taggaggagt gcttaaagtt ggtgcagata
     4321 cagtttcagg agcctatgct atagttatga cagaaatgaa tttaccagtt gagcgtttct
     4381 cagatagtat atactacatg gcaactggat ttacaaagat gtttggttat ataggtatag
     4441 gagctgcctt tatttatact gcatataagc aaaataagaa gaaagttaag gcaacgatta
     4501 ttccactaat aattacggca gtattagcct ctgtaacaga accattagac tttatgtttg
     4561 tattctgtgc accattttta ttctttgtac atgcagttat agctggtctt tttatagctt
     4621 tattaaaaat atttgatgtt actgcattat gtggagggaa cttattagta tcaattataa
     4681 tgaatactac agtaggcgta gaaaaaacac actggccagt aatgatgata ttaggacttg
     4741 cacaaattat tgtatatttt gtagtattta gttttttaat taagaaattt aattataaaa
     4801 ctccaggtcg tgaagatgag agtacagcta caggcgaaga acaatcaaaa caacttaatt
     4861 tagatgcagg gatagaaaat ataattgatg ggttaggcgg aaaagaaaat attaatacag
     4921 tagaaaattg catcactaga ttacgtgtta atgtaaaaga tgaaagtaag attaatgaag
     4981 atataattaa tttaacgcca aatagcggga ttgtaaggaa aggaaaagat atacaaataa
     5041 tttttggact tcatgtgcat gaggtaagac gtgcagtaga agatttttta gaagcttatt
     5101 aaagatatat atttatatag caagggtaaa taacagcttt aagtgctgtt tactatttat
     5161 taaagataca tataatttat agaggaggaa taattattat gattattact attgttggtg
     5221 gaggaagcac atttactcca ggtattgtta agtcaattgc attaagagaa aaagagttag
     5281 agcttgagga aattcgctta tttgacattg ataaagaacg ccaagataaa gtagctgtag
     5341 ttgtcaactg gatactaaaa gaagatgtta aaacagatgt taaactaact gtaactaatt
     5401 gtgaaaaaga agcgtataca gatgctacat ttatatttgc acaaatgcgt gttggtaaat
     5461 atgaaatgcg tgagcaggat gaaaaaattc cattgaaata tggttgtgta ggtcaagaaa
     5521 cttgtggttg tggagggatg gcatacggac tgcgtacaat acaaccaatg ataaaggtta
     5581 ttgatgatgt agagaaatat gctaaaaaag attattggat attaaattac tctaatcctg
     5641 ctgcaatcgt ttctgaagct tgtcgtaagt taagaccaaa tgcaaggatt ataaatatct
     5701 gtgatatgcc aatagctatt atagatatga tagcggggtc attaaatatc aatgacgttc
     5761 ataatatacg ctatgattat tttggtctaa atcattttgg ctggtttact tcaatagatt
     5821 ataaacatag agacttaatg gaagagatta aggaatacat aaaagaaaat aaaatattat
     5881 taccaaaatc ttatatagaa aacttaaata aaagtcgtag tacaggaaat cgccatgctg
     5941 taagtagctg gatacatgtt tgggaatctg tttatacaat gttagaaagc ttcccaaaat
     6001 atctgccaaa cacttattta acatactatc ttttaagtaa agaaacagtg gaacattcaa
     6061 acataaatcg tactcgtgca aatgaggtta tggaaagccg tgaaaaagac ttatttgaag
     6121 gtgtgagaca ttacttagaa actggagaaa tttctgaaaa agcattttat gcaggttcac
     6181 atggagattg gattagtgat ttggcagttt caattaaaaa tgatacacgt agtcgttttt
     6241 tagttataac tgaaaatcgt ggagctatac caaacatgcc atatgatgca atggtagaaa
     6301 ttccagcata tattggtaaa gatggtccag aagttattgc tcgaggtcca atacctctat
     6361 ttcaacaagg attaatgatg caacaactta acagtgaaaa attgatagta gaagcttata
     6421 tagaaaatag ttatgaaaaa gctttacaag cattcacatt aaataaaact attccaagta
     6481 tgaatgttgc taaagaaatt ttggatgaaa tgatagaagt taataaggaa tactggccag
     6541 aattaagata gtaggataaa ggaaagggtt tttatttaat aattagatat gttttcagag
     6601 tcttttatca aaatgtattc acaaacatat ggcaatatat taaaataaag agtgatattt
     6661 ttagaagaat ggggtgatgc aatgaacatt ataaataaaa ttttttggga agaagatagg
     6721 attggcatga ttacaaataa tttagaggca gatacacata gccactgtat gttacaactg
     6781 ttcttaggga tagaagacag cattgaaata acagtcaatg aaaaattagt taaatgtaat
     6841 tgtatcatag tagataaaaa tatctctcat tcattttcag caagaaaaaa agtttattac
     6901 tctgctatta ttgaacctac atctatatat gcagaacagc ttacctcaaa aatgaatgat
     6961 tttggctact ggatatgtga caatgatgga ttagagaaat tacgtcaaca aggaactttt
     7021 ttgatagata actctagtaa agaacaatat ttaagattta tggaaatgct taataactat
     7081 ttgaatatac ctataactct caaacattat gatgacagaa ttactgaatt gctcaattta
     7141 ttacgtactt gtaattgtga taatcatact atttcaagtt ttgcagataa agtgtcccta
     7201 tcagcaagta gattatcaca tctgtttaaa gaacaaatag ggataccttt aaaaagctat
     7261 atcttatttc atcaaatgga gtgtgctttt agagaattac tttctgggaa aaatgttacc
     7321 gaagcttcta tgactgctgg attcgatact ccatcacact ttgcagggac agttaagcga
     7381 atgatgggaa tgcctgtttc tttatctttg aaagatagcg aatttttgaa agtatattaa
     7441 atattctcgt tatataatga agttgtacta agaaaggggc tttgttatga ataaagaata
     7501 tttagaggaa acaaaaatgt taaattataa tgaaccacaa cttaagcttt tggtatcatc
     7561 aaagaattgg ttggaattag atgattttca taaaataaaa tctatttatg agtttgtaca
     7621 gaatgatatt ttatttggat ataatgcttc tgatatgtta agcgcaacac aggtattaaa
     7681 tgatggtatg ggacaatgca atacaaaagc tactctttta atggcattat tacgtgcagt
     7741 gaatatccct tgtaggcttc atgcttttga tgtaacaaaa gattttcaac gaggagctac
     7801 atccaaattg atttcactat tagcaccaaa atatattctt catacttggg tagaggtatt
     7861 ttatcaagat aggtggattg ctcttgaggg tgtcataact gataaaaaat atttagaagc
     7921 aattcagaag aaatttttta atcatggtgg aacatttaaa aagtatgcaa tagctacaaa
     7981 tgatttgaaa aatacatcta ttgattggga tggtaaagat acttttatcc aaaaagaagc
     8041 tatcgtgtat gattatggta tttttccatc accagacgta tttttttcta ctcattcaca
     8101 acatatgtca aaattaaaaa atttcatata tgtacatctt atcagaaaaa taatgacaaa
     8161 aaatgtatgt aaggctagaa acaattatat tgataaaaac gaatgagtgt ttaagaggta
     8221 actatatagg aaatatatag aataattaaa actgtactgg ttaaaatgaa taaaaaggag
     8281 ttatcacaga gcaaacttaa attgttcata ggataactcc ttttctgtat taacttctaa
     8341 taatttgatg tctttttata aaacatatag atacaattaa accagtaaat gcaagagtag
     8401 ctgctattga aaatgcaaca tctattccat gaatgttaga cagcatgttt gaactcatat
     8461 gagctttctt agtagcactt gtcattatag taataagtat agcagaacct atagctccag
     8521 atatctgacg gagtgtgtta ttaattgctg tggcatgaga tataagttct ctatctaatg
     8581 ttttaattcc ccatgtagtt aatggcatta aaaccattga aataccaatc atacgaatac
     8641 aatacatcaa tgacacatga attaaagatg tatctctgcc taaaaaagcg aaggataaag
     8701 ttcctaaaaa caaacatcct gttcctaata ttgatagagc atgaggtcca tatttatcta
     8761 aatgacgtcc tgcaattgga ttgagtacaa ccatcaatat agcaccaggt aacattaatg
     8821 aacctgaaga taatgcagaa aatcctctta cagattgaac gtacagagga atcattaatg
     8881 tagcagacat cattgaggca tatacaatca taattaatat tgtagaaata agaaatggtt
     8941 tatttttaaa aaccctcaat tctaaaaaag gttctttaga ttttaattga cgcaatgtaa
     9001 ataaaattaa tgacataatt cctattaata ttggtaaata tgtggcaata ttagtccacc
     9061 cataattacc ttgattagta aatccaatta ataaaccacc aaaacctaga gatgatagta
     9121 ctattgaagg tatatctaat tttagttttt gagtttcccc aacatttctt aatagaataa
     9181 atgatagtat aacatctaat atagcaatag gggaaagaaa cagaaataaa ctatgccaac
     9241 caaatgaatc tactaaccat cctgataaag ttggacctat tgctggagca aatcctactg
     9301 taataccaac taaactcata gctgcaccac gtttttcctc agggcataaa tagagtataa
     9361 taacttgtaa aagtggcatt agagaaccag aacccattgc ctgcaacaca cgagcaatca
     9421 acatagttga aaaattatta gaaaaaagtg caataataca tcctatagaa aagatagaca
     9481 ttgatgtaat aaataacttt ctagtactaa atcgatttat taaataagct gttgtgggta
     9541 caataatacc tgcaataagt agataaattg ttgttaacca ctgaccaata tcagcagtaa
     9601 ttttaaaatc tgacattata ttaggtaatg ctgttgacaa tagtgtttga ctgagagaac
     9661 ttataaaagc accagtcatt acaattgcta ttattaaatt tcctttttta ttgttacttt
     9721 gggtttccat aatttataac gtctcctttt ttaattgatt tttaatgctg ttcaataaaa
     9781 gtacttttgg tcttaaagtg tcttcttttt ctaaatatgc aatcattgca tccataatta
     9841 aagaatcata ttgttgattt tttccttcga cgtataattt ttttattgct tctagtaatt
     9901 taggattttc aaggagagaa gtatcagttt tgattagttc ttctatatct tgtatcgttt
     9961 taaaaaatag cgatttatta tgaagggaaa agtaaacttc atcttcttgt ttatgtaaaa
    10021 acattgattt ttcaattaaa ggatttagat tatccatatt ttctacaact aaatctagaa
    10081 tttgtatagt aaatttggca ataaagtata catttatttc tcttttttgt acatgaataa
    10141 tgtatataaa ctgatgaatt agagcatcca ataagaaaac taaatcaaat ataatttttt
    10201 ctattttatc accatagtta tcaaataagc ttagtttgta ttcattcaat aagttagctc
    10261 ttgattttgt ttgcaattta cttgtagcct tacgttgctc ttcagaaaag ttttctctta
    10321 tataggtatt tatggtagtt ctagaaaaaa tatttttcca tacagcaatt atctttctgt
    10381 ttaacttttc tgatgatgga atgtcattat tttcatttat ggaatgtata atttctaaaa
    10441 acttttcatc ataataattg attatttcgc agataaaact ttccttagtt tcaaaatgct
    10501 tgtaaaaagt agctttagac attttacact cttttacaac atccgatatt gaagtatttt
    10561 gaaaaccttt ttggtcaaaa attttttgtg caatagttat aatgagcctt tgtttttctg
    10621 tcactttatt atctcctttc acgcaaaaac gtttagtact atatagtacc tacaagtttt
    10681 gataaatata ttatacatca tttggaaaaa aaatcaatat atagactgta atatgaaatt
    10741 tttgaaaaat ttgttgttat ttttagtcgt ataaaaaaga aacttaagag tttcttaaga
    10801 attgtttaaa gaagttgcaa taaattagtg gtatgattat agtatgaaag agaatgtctt
    10861 gaaatttttc tgtatcaaat tccaattatg gaggtgtagt atatgaaaaa gaaaatttgt
    10921 gtagcaataa tttttgctct atttatagtt tttgtagaaa tgtttattga tagctctagc
    10981 gaaggaagta aaattatgac agctgaatta agtaaataca ggaaaggcgt aaatgtatta
    11041 tcaataaaca atattatagt agcgaataaa aaatatagtt tgcccaaaga ttattctcct
    11101 caggaaagca gtgaagcgag agatgctttt tataaaatga ataaagatgc tcaaaagtca
    11161 ggattaaatt taaaagcttt tagtacatat agaagttatg aatatcaaga tagattattt
    11221 aagtcatatg taaaagaaca tggagaaaaa gaagcaaata gattttcagc taaacctgga
    11281 gagagtgagc atcaaactgg acttgctttt gatattggag gagatgacca gtcttgttgg
    11341 gcaaataaaa agttcaacaa tacaaaagaa gctaagtggt tgtatgaaaa tgcatataaa
    11401 tatggtttta tacttagata tccagaaggt aaagagaata taacaggata tatgtatgaa
    11461 tcttggcact atagatatgt tggaactgaa catagcaaaa actttgcaat gaataatttg
    11521 actttagaag aatatttaca tataaattaa gagatggttt gttaaaatta ggttaaaata
    11581 aaaaataacc tagactatta gctgtgtaaa gtgatataat gtaaatacaa tttaagataa
    11641 ctactcgaag gggagtagct tacacgaaag tggtaattaa tcgtcaagac agtagaaata
    11701 ctcggttaat tagcaaggga ttccttgata gcaagacctt caaaataagc attaggtatg
    11761 tttgttttgg ggtctttttt tattcaagga atttttattt aaaaaaattt aaatttcaag
    11821 tatacataag agcccccaaa cataaaataa atttattgaa gaggagggca acaaatgtct
    11881 tacaaaagta cttatcaaga agtaattaaa aatttagact caagtttatc tggtttgagt
    11941 aaagaaagag cagaagagtt gttagaaaaa aatggagcta atgaattaaa agaagcagat
    12001 aaggttccta cttataagct tttcctagaa agttttaaag acccattagt aataattcta
    12061 ttgatagctg ctttagtaca aatattttta ggtgaaacag tggagtctat aataattttt
    12121 gcagttatta ttataaactc tgttctgggt gttgtacaaa caaaaaaagc agaaagttca
    12181 ttagaaagtt tgaaaaattt atctgcacca aacgcaaagg taataagaga caataaaaaa
    12241 atgacaattc cagcaaaaaa tctagtagtt ggagatattg tattcttaga agctggcgat
    12301 tacataccag cagatggaag gcttatagaa gcacaatctc tcaaggttgt agaagggatg
    12361 ttaactggag aatcagaacc agttttaaag catatagaaa aaataagtga agatgtaagt
    12421 ttaggtgacc agaaaaatat ggtatttagt ggtgctgtag ttgtttatgg tagaggttct
    12481 tttgtagtga ctggaacagg tatgaatact gagatgggta aaatagctgg gcttttagaa
    12541 agtgctgaaa ataaacttac accattgcaa caaaaaatag aggattttag taaaaaactt
    12601 ggtgtaggta ttgtaatact tgctctttta atatttataa ttcaagtagc aagaaattac
    12661 tttatggctg gaaatggaga aatgacacaa attattttag attcattcat gttctctgta
    12721 gcagtagcag tagcagctat accagaagct ctatcatcaa tagtcactat agtattggct
    12781 gttggaacaa atagtatggc aaaaaaacaa gccataataa gaaaattacc agcagtagaa
    12841 actttaggtt caacaagtgt aatatgtact gataaaacag gtactcttac acaaaataaa
    12901 atgacagtag ttgattatta tatgtatgga actaataatg atgagttaga taaagaaaaa
    12961 gtaaactatt cttatcatgc taaattattg actatggttt ctacattgtg taatgactct
    13021 catataacta gtgatggtaa agaagttggt gacccaacag aagtggcttt aataaattat
    13081 tcaagtaaaa atgatttaga ttatgataaa ttaagaagta agtatatcag aataaatgaa
    13141 ataccatttg actcagacag aaagcttatg tctactgtaa atagtatata tggagattat
    13201 ataatgttta caaaaggtgc tccagatatt gtgtttagta gatgtaaata tgctttgaaa
    13261 aatggttcaa aagtagatat aacagatgag ataatagaag aatataaaca taaaaatgaa
    13321 gagttctcaa atagagcact tagagtatta gcttttgcaa taaaagatgt cccagaagaa
    13381 gattttatac cttcaattga tgacgaacat gacatgacat tggttggaat aatggcaatg
    13441 atagacccac caagagaaga agtaatggat gcagtaaaag aggcaaagag tgctggtata
    13501 aaaactgtta tgataactgg tgaccataaa acaacagcag cagcaattgc aaaagagatt
    13561 ggtataatgg aagaaggaga cttggctctt actggaaaag agcttgatgc tttgagtgaa
    13621 gaacaactat atgaaaaact agaaaatata agtgtatatg ccagagtctc gccggaaaat
    13681 aaaattcgta tagtaaaagc atggcaacat aagaataata taacagcaat gactggtgat
    13741 ggtgttaatg atgcacctgc attaaaacaa gcagatatag gaatcggtat gggaagtggt
    13801 actgatgtag caaaagatgc ttctgctatg gtattggttg atgataactt tgcaagtata
    13861 gtaaatgctg ttgaagttgg tagaacagta tacagtaata taataaaatc aattacttat
    13921 ctatttgcag gaaacttagg agctatagta tccatagtat ttgcagtgtt tgcaggttgg
    13981 tcaaatccat ttacagcttt acaacttttg tttataaact tagtaaatga ctctttacca
    14041 gcaattgcat tagggtttga aaagtcagaa aaaaatataa tgaataaacc tcctagacac
    14101 ccggatgaaa gtatactagc aggaggaaca ttgaaggcag ttgctataag aggttgtata
    14161 ataggtgtag ttacaataat tgcacaatat ataggattac aagtatctcc agaacttgga
    14221 gtagcaatgg cattttcaac attgatatta gctagaataa tgcaaacatt acctgcacgt
    14281 tctaataatc aaacagtaat aaaattaggt tttttcagca ataaatacgt tataggagca
    14341 gttttagtct gtcttgggct ttactcttta acactaatac cagtgctaag aggaatattc
    14401 tctataccaa caacgtttgg attgtaccag ttggggattt gcctagggtt agcattaatt
    14461 tcatcaatat taatggaact taacaaatta ataaatagaa gtgaagtaaa ataatcaaaa
    14521 aattaaaggg catattgatt ttaacaatat gccttttata aatagtctaa tttattctat
    14581 attatcgatt atttggaatg cttttataac ttcaggtaat tctttttctt caggcattct
    14641 aaatcttata aaatttttat caagtccagt aaaatcactt ccagatataa ctcttatttt
    14701 taatttagca aactcttgtt gtaaatctat gtccttgttt ttatgttcaa ctgtcataat
    14761 tgatacagta tcactagttt cagctatatt aaggtttttc catggaatta ataattgatt
    14821 ttttatatct tttgtttttt cttttaattc ttctataaat tgaacatcat ctaataaatt
    14881 tgctgcaata ctacgagata tttcagacac ttcgtaagga gtagaaatct ttttaatagg
    14941 gctaacaagt tgttctggaa gaactgcata tcctgctctt agtcctgcta aaccaaatcc
    15001 ttttgaaaat gtttttaaag caattacatt atcataatta tttagaagct tgacagctga
    15061 attttctttt ggcatgtatt caccataagc ttcatctacc ataactgcta tatcatattt
    15121 agctgcttcc cttactatat tttcaattga tgataaaggt ataatttgtc ctgtaggatt
    15181 gtttggattg tctatgtaaa ttactttata ttctggattt attttttcaa taaattcttt
    15241 ttcattaaac ttgaaattat cctctttctt taaaagaaca taatcataag ttgcaccatg
    15301 catttttata tctgtctcat attctgagaa ctgaggtgaa tatcctaaaa ctttatctcc
    15361 cttttcaata aatagtctat ttaaaaggta aataacatgt atagaaccat ctgccaaaca
    15421 aattctgtca gtatctaatg ctataaaatc tttccaataa tcgactatat tatcttttaa
    15481 agcaattgaa tgaggatatc ctcttatcat atcaaatttt aaagcattaa aagcttctac
    15541 agctttgttt gagtaagaca cggtatttat accttcacca cagtctatct cgaatacatc
    15601 ttctgaatgt ccagattcta ttgcataact ttttttcaac tgcatttcaa tagctttgtt
    15661 aatttttagt ttcatacttt atcactcctt atttagtcaa taataaatgt tataaaagtt
    15721 gctagattta aaatattatg aaaatatata atagtaaaaa tgaatataaa tattataata
    15781 gctcact
//
LOCUS       DAYAOX010000089        15697 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.089, whole genome shotgun sequence.
ACCESSION   DAYAOX010000089 DAYAOX010000000
VERSION     DAYAOX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.089"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     regulatory      <1..94
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            277..849
                     /locus_tag="ACGI57_002085"
     CDS             277..849
                     /locus_tag="ACGI57_002085"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFG9717162.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            913..1563
                     /gene="fsa"
                     /locus_tag="ACGI57_002086"
     CDS             913..1563
                     /gene="fsa"
                     /locus_tag="ACGI57_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFG9717163.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            1715..4639
                     /locus_tag="ACGI57_002087"
     CDS             1715..4639
                     /locus_tag="ACGI57_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9717164.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVMKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            5228..5686
                     /locus_tag="ACGI57_002088"
     CDS             5228..5686
                     /locus_tag="ACGI57_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9717165.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            5747..6022
                     /locus_tag="ACGI57_002089"
     CDS             5747..6022
                     /locus_tag="ACGI57_002089"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9717166.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            6081..7355
                     /locus_tag="ACGI57_002090"
     CDS             6081..7355
                     /locus_tag="ACGI57_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFG9717167.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            7485..8537
                     /locus_tag="ACGI57_002091"
     CDS             7485..8537
                     /locus_tag="ACGI57_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFG9717168.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            8537..9568
                     /locus_tag="ACGI57_002092"
     CDS             8537..9568
                     /locus_tag="ACGI57_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFG9717169.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            9660..10472
                     /locus_tag="ACGI57_002093"
     CDS             9660..10472
                     /locus_tag="ACGI57_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFG9717170.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            10475..11395
                     /locus_tag="ACGI57_002094"
     CDS             10475..11395
                     /locus_tag="ACGI57_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HFG9717171.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            11570..12016
                     /gene="rpiB"
                     /locus_tag="ACGI57_002095"
     CDS             11570..12016
                     /gene="rpiB"
                     /locus_tag="ACGI57_002095"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HFG9717172.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            12046..12711
                     /locus_tag="ACGI57_002096"
     CDS             12046..12711
                     /locus_tag="ACGI57_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFG9717173.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIEPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            12821..14323
                     /locus_tag="ACGI57_002097"
     CDS             12821..14323
                     /locus_tag="ACGI57_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFG9717174.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(14635..>15697)
                     /locus_tag="ACGI57_002098"
     CDS             complement(14635..>15697)
                     /locus_tag="ACGI57_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFG9717175.1"
                     /translation="VFFSNLLNMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFFMYI
                     YYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVIGD
                     DEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDMLKN
                     NDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQSIV
                     EFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDMFI
                     KEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENAHM
                     EIIIDADLFKVMLTFRRVN"
BASE COUNT         6303 a         1669 c         2907 g         4818 t
ORIGIN      
        1 taatatattt acctgtatat gtttgataat atggttcaaa tgtttctact aagctaccat
       61 taatagcttg actacaggag tgaagaagat atagtttata cttattttaa attatatatt
      121 aagttaatta ctttgaattt taagctcctg tctatgatat agacaggagc ttttttagta
      181 ttttaatata aaatacttac aagaatagat agcaaaatta gaacaaatat aattttttta
      241 ttaatataat tttagaaagg gctaggtgga agttcaatga aggctttaca agaaaagata
      301 ttgagagaag gcagtgtttc aggaaatgat atactaaaag ttgatagttt tttaaaccat
      361 caaattgatg tagctttttt aaatgaaata ggaagagaat ttaaagagag atttaaagga
      421 gaaaaggtgg ataagatatt tacaatagag gcttcaggaa tagctatagc atcaattgtt
      481 tcacagtatt ttgataatgc acctgtagta tttgcaaaaa aatcagaatc taaaaatttg
      541 gataaagatg tatatgaaac taatgtatat tcatttacta aggcaagaga gtattcagtt
      601 aaagtttcta aaaagtacat aaacaaaggc gaaaatatat tgatagtgga tgattttttg
      661 gcaaatggaa gagctgctct tggacttaaa gatttaattg agcaagcaga agctaactta
      721 gttggagtag gaattgtcat tgaaaaagga tttcaagctg gaggagcact tttaaaggct
      781 aatgatgtaa gattagagtc tttggcagta gttgaatcta tagataatgg aactgtaaaa
      841 tttaggtaat ttatgatatt atatacttgt gtttagcaca tattttaact atgtaattat
      901 ggaggatttt ttatgagatt ttttatagac acagccaata ttgaagaaat aaaagaagca
      961 aatgatttag gtgtaatctg tggtgtaact actaatccgt ctctaatagc taaagaaggt
     1021 agagatttta tagaggtagt taaagaaata agtgaaatag tagatggacc aataagtgca
     1081 gaagtaataa gtttagaaca taaaggtatg atagaggaag cagaaaaatt atctaagata
     1141 cataaaaata tagttataaa gatacctatg acagcagaag gtctaaaagc ggtgaaagta
     1201 ctatcaagta aaggtataaa gactaatgta acgcttatat tctctgctgg acaagctctt
     1261 ttagctgcta gagctggagc tacatatgta agtccttttg ttggtagatt ggatgatata
     1321 tcacaaaatg gtcttgattt aattgaagaa atcgttgata tatttagtgt aaatagtatt
     1381 gaggctcaaa taatagttgc tagtgttaga aatccaatac atgttttaca agctgctaga
     1441 atgggagcag atatagcaac agttcctctt aaagttataa accaaatgat taagcatcct
     1501 ttgacagaca aaggaataga tagctttatg aaggattggg aaggtgcttc tttaaaatta
     1561 taattataaa ctacgcttta taagtatatt aaagaggtta ttatggaaat tctaagtaat
     1621 aaggctaggc tattttgcct agtcttattt tggtgtataa tatattgaaa catattattg
     1681 aaatagatta attttttaag tgaggaggcg aaaaatggat aaaatattaa gagagataca
     1741 aagagaagat aaaaaaaatc catatactga ccaagaactt gctgaaatgt taaatgtagc
     1801 taggtctgaa gtgatatcag ttaggaaaaa aaataatatt ttagattcga gagaaagaag
     1861 aaaaaaaata ttaataaaag atatatcaaa aattgtaagt gaaaatcctc aaatgtctga
     1921 aagaaaaata actgaaatat taatagaaaa tggatataat atatcgagaa gtgcagtttc
     1981 aaaattatta aaagaggaaa atttaaatca gtcaaagatt gggattaagg ataaagaccc
     2041 agcatcatca ggaaagtatg tgaaggtgag taaagttgta gaagaagatg gatttgaaga
     2101 attgataggt ataaagggaa gtttgaaaga aaaggtaaat ctagctaagt cagcaataat
     2161 gtatcctcct aatggccttc atactataat atatggagaa acaggagtag gtaaaagtga
     2221 attagctaca tgtatgtata aatatgctat aaaaaacaac attaaagaag agaacagccc
     2281 atttatagtg tttaactgtg cagactatgc agaaaatcct aatctattga tagctcaact
     2341 ctttggggta gtaaaaggtg catatacagg agcagatgca aatagagaag gtcttgtaga
     2401 acaagctaat aatggaatat tgtttttaga tgaaattcat agattgcctg ctgctgggca
     2461 agaaatctta ttttcattaa tagatagagg agaatttaga aggttaggag aaagtagttc
     2521 taatagaaag gcaaatgttc tcataataag tgctacaaca gaaaatccag attctaattt
     2581 acttcaaaca tttagaagac gtataccaat ggtaataaat ttacctaata tgagtgaaag
     2641 accaaaatct gaaagatatg aaataataat aaagtttttt gaaagagaag caaagcgagt
     2701 aaacaagaat tttataataa ctaaagaagt tatgtgtgca cttatgaatt ataagtgtac
     2761 tggaaatata ggtcagttaa agagtgatat acaagtaact tgtgctagag cttttagtaa
     2821 atcgatattt tctagcgata aagtaattat agatttagat agtttaaggg attatataaa
     2881 aagtggatat tatgattgta attgtaaaaa tgaaaagtat tctgagtttt cagttgagga
     2941 tatatatata gatattagtg aattggtgag tggaaaaaat agaattttat ctgataatga
     3001 gataagcgag atatataatt atgcagaaaa agagcttaag gttttggaaa gtaagtgctt
     3061 ttcagaagaa gagttaaaaa atgcatttat agaaaagtta gataagaagt ttaatgctat
     3121 taaaaacaat aaaaatttat ctgatagaag aagtcgaata gactggggag gacaattaaa
     3181 agagtctaca ctggagataa tggataaggt aattgtattt attaaggagc agtttaagca
     3241 tgtaaatcaa ggtctgtact tggctctagc aattcatatt gagcatgcta taggtaggat
     3301 aaaagatgga aaaactataa taaatccgag tttagataaa ataaaagcat caatgccaat
     3361 ggaatatgag ttgtcaagat atataatggg tatagtagaa gatttgacac aagtaacatt
     3421 tccagaagat gagcttggat atttggctta ttatataaat aaattttgtt ataatgagga
     3481 aagtataaaa gataaagtta aagtagtaat agttacgcat ggtaaagttg gaattgaaat
     3541 gtctaaagtt gtaaatcata ttttaggtat agaatgtaca ctaggtatag aaatagcact
     3601 tacagattca ccatcagagg ggatagagca tgtattagaa gaacttcaaa agatagaagc
     3661 tagaaaaggt attctagttc taattgatat gggttctttg gtaattcttg gtgatgaggt
     3721 tgaaaaaagg cttggaatta gatgtaaaac tgtaaataga gtagatacat tattggcaat
     3781 ggaagcagga aaacttgcaa caattgaagg aaaaagttta gatggtataa tagctgattt
     3841 aaagaaaaat aagaattatg ctatgataaa tacaaataaa tttagttaca gaaaaaacga
     3901 gtatggaaag aaaaatgtaa tcattacttt atgtttgagt ggtgtaggta cagctttaaa
     3961 tttaaaagag catatagaaa aacaaattaa ggaatatgac actttaattg aagtaaagcc
     4021 agttgctttt ttaaataata atttagaaga agagttaaat gatattgaat ctgaatataa
     4081 tattattgct atctgtggaa ctattgatat agattataaa aacgtgcctt ttatttctta
     4141 taatgaggta cttggtaaaa atggtattaa taaagtatta gagcatttaa atagtaaaca
     4201 accagcttta aatattgaaa accagttgaa taatttaata catgaagatt tgattttata
     4261 caattttgaa ggaatttcaa aggagtatat aattgatact cttgtttcaa aacttgaaga
     4321 aggtggatat gttgactcaa agtatatatt aagtgtatat aaaagagaag caatgggaag
     4381 tgttgtaatg gcctctaagg tagccgtgcc acatggactt ccagaaaacg ttatgaagcc
     4441 tgcaatagca atcgcgaggt tgaataaacc tatagtatgg gacaataaat ttatggtgga
     4501 tttggtggta ctactggcac ttaaagaaaa taataagaaa gaaatacgaa gcttattttc
     4561 aaagattaat gaccagcata ctttagaagt tttattaaat actgaagata aaaatgaaat
     4621 aaaagaactt ttaagctaat tattagtacc gagaagaaag acaaaaaaat ttgctttagt
     4681 ataaaattta aaactactca attgctaagt agttaattac tctatataag gctagttcta
     4741 tgaaatttat ttatatgatt ttatagaatt agcttttttc ttggtaaata aaatatataa
     4801 atttatttat gtaaaaagta aattttattg gacaattcta taaaaatttt tcctatattt
     4861 cttataaaat atagaatata taaaaaatat ataaaaataa attaaattca taataaataa
     4921 ttttataaaa tagttgttaa acataaaaaa ccatttgtaa aaaacaaaaa aatcatttct
     4981 ctaacaaaaa aaaccacaaa aaatataaaa aagtatggct agttaagtga ttaacaagta
     5041 aaaattaaaa taaaaatcaa aaaaaattta tcaatgaaag tcagctaaaa tcctggaaga
     5101 gagcattatt tttgggaaaa cattttcttt tgtttcacaa aaaaagccac tttttggcac
     5161 acaacatgct ttatataaag tcaagaaaaa ctaaggtata agacaaaggc aattaggagg
     5221 tgtaaatatg gagtttataa attttgtaag tgataaagtt atagacctta aaatggattg
     5281 taaaacgaaa gaagatttct ttaaagaaat acataataaa gtttttgatt taggctttgt
     5341 aaaagaagaa tttggagaaa aaatattagc aagagaaaat gtatttccaa caggtttaaa
     5401 tttaggtgat tatggagtag caataccaca tacagatgcg gaatatatta aagagcaatt
     5461 tatatcagtc tgtactttta atgaaccagt agtatttagt tctatggaag accaagatga
     5521 aaaagtacct gtaaatttgg ctttcgtatt aggacttaat caaccacaca gtcaattatc
     5581 tgtattaaca gaattaatgg gaattatgca aaataaagag ttagttgata agttgatgag
     5641 ttctactgat aaagaagaag ttttaaagac actaaagtct ttataaaaat aaaaaagtac
     5701 taaaaataaa aaatataaaa ataaaaatta ttaggaggaa ataaaaatga aaaaaatatt
     5761 agtagcatgt ggagcaggaa tagcaacatc aacagtagtt tgtgacaagg tagagagatt
     5821 agttaaggaa aataatatac aagcagaagt tatacaatgc aaaatagctg aatgtgcaac
     5881 aaagcaagaa ggagcagatt taatagtatc aacaactata ctaccaacaa cttataatat
     5941 accagcaata aaagcaacag catatataac tggagtaaat acagcagcat tagataaaaa
     6001 aatattagat gctttaaaat aatcctatca tagatacatt gaaataatat tttataaaac
     6061 agaaaaagga ggatttataa atggataaat tattagcagt agtacaatac attttaaata
     6121 tgggaccgac agtaatacta ccaataacaa ttgtagtact aggaatgata tttagaatta
     6181 agtttacaaa agctttaaag tcaggtctta caattggtat aggatttgtg ggtataaact
     6241 tagttgtaaa cctattaaca tcaacactag ggccagcggc tcaaggaatg gttgaaagat
     6301 tcggacttaa tttaacagta atagatgttg gttggccaac aacagcatca gcaacatggg
     6361 cttcaccagt tgcaccaata ttaataccag tttgtttatt agtaaactta ctattattat
     6421 acttcaataa aacaaagata ttaaacgttg atatatggaa ttattggcac tttatagtag
     6481 caggtgcagt tggatatata gtaacaggaa gttatgtttg ggctatattc tgtgcaatac
     6541 taatggagtt attagtactg tttattgcag aaaagacagc aaaaaatgtt caggagtttt
     6601 atgatttaga aggtatagca ttaccaactg gttcaacagc ttcatttgct ccacttggat
     6661 atttagtagg taaatttgtt gaaaaaatac caggaataaa taaaatacaa gcagacccag
     6721 actctataca aaaaagattt ggtatatttg gagaaccaat gatgatgggg attatactag
     6781 gtataatact tggagcactt gcgggatatg atgtagcagg aatattcaat gtaggtatat
     6841 caatgggtgc agtaatgtta cttatgccta gaatggttag aatattaatg gaaggtttaa
     6901 ttccaatttc agaatcaata agagatttct tacaagcaaa atatggagac agagaacttt
     6961 atataggtct ggatgcagca gtagcaactg gacatccagc agttatatca acagctctta
     7021 tacttgttcc tataactttg ttcttagcag ttatactacc aggaaacaaa gttttaccat
     7081 ttggagactt agcgacaata ccattttata tggcatttat agtgtgtttt agaaaaggta
     7141 atatagttca atcagttata acaggaacaa tagtaatagc tatatcactt tatatggcta
     7201 caaactttgc agatgttcat acacaattat tacaacaagc aaatatgatg ccagaaggaa
     7261 caacaatggt tacaagtata gatacaggtg gaaacttgct aaaatgggga atattgaaat
     7321 tatctcaatt agtttcttca tttggaatat tctagtatgt agttaataga gattactctt
     7381 ttttagtaaa gtatgcgaaa atttaacatt tagcagctac taaaaaagag taatattttt
     7441 tataaatttt taaaaataca aaatacaaga ggaggagatt tatcgtgaaa gcagcagtat
     7501 tacatggcac aaatgatatg agatttgaag atatagaaat aaaaccatgt gaaagtgatg
     7561 aagtaaaaat aaaagttatg gcagcaggaa tatgtgggtc agatcctcca agagtattaa
     7621 aacattggaa atatccagtt ccagctatac caggacatga gttttctgga gtaattgctg
     7681 aagttggtaa ggatgtaaaa aatgtaaaag ttggagatag agtagttgca attcctttta
     7741 taccttgtaa tgaatgtgaa tattgtaaaa gaggattatt ttcattgtgt gatgaccatg
     7801 gaatgttagg agctaaaagt tttggagctt ttgcagagta tgtaaatata aaagctacaa
     7861 atgtacttcc aataggagat atgaactttg aagatgctgc aatgatagag ccactagcag
     7921 ttgctatgca tggagtactt aatataggtg ttcaagttgg agacactgta gctgtaatgg
     7981 gtagtggaac aatggggcag ttggtaatac aaggacttaa aatagcaggg gcaggaacaa
     8041 ttatagcagt tgatatatct gataataaat taagagaatc aaaggaatta ggggctgata
     8101 taataattaa tgctaaagat attaacccag tagaaaaaat aaaagaactt actggtggaa
     8161 aaggtgttga catagctctt gaatgtgcag gttcaaaaat aactcaagaa caatgtttac
     8221 ttataactaa aaagaaaagt aagataggat ttttagggat agcatattca gatataactt
     8281 tatcagaaga agcatttgaa aatatattta gaaaagaact agaattaaaa ggtttttgga
     8341 actcttattc tgcaccattt ccaggacaag aatggactaa gggaatcaat ctagttaatg
     8401 aaggaaagat aaaattaaaa gaaatggtat ctcatagatt ctcacttgaa gatacttaca
     8461 aagcatttga aatgataaga gatagaaaag aagaatttaa caaaatatta attttaccac
     8521 aaggggttga aaaataatga aagcagtagt taaaactaaa ccaggctatg ataatgtaga
     8581 agttttagaa gtagaagaac caaaagctac aggagacaag gttaaaataa aagtagaata
     8641 tagtggtata tgtggttcag atatacattc atttaaaggt gaatatgcaa atataaaagc
     8701 cccagtaaca ttaggtcatg aattttctgg aattgttgta gaagtaggag aagatgttaa
     8761 aaatataaaa gtaggagata gagttacatc agagactact tttgcaactt gtgagaaatg
     8821 tatatattgt gaaactaaag attataactt atgcccaact agaaaaggta ttggtactca
     8881 agtaaatgga agttttgctg agtatgtatt atcaagagaa gaaagcatac atgtacttcc
     8941 tgaaaatgta tctttattat cagcagccct tacagagcca ctagcttgtt gtgttcatgc
     9001 agctcttgaa aagactacta tagaaagttc tgatacagtt ttaataatag gaccaggacc
     9061 aattggttta ttattagctc aagttgttaa atctcaaggt gctacagtta taatgtctgg
     9121 tgttacaaaa gataaggaaa gattagaaat agctaaaaat cttggagtag atagaactgt
     9181 aaatgtaatg gaagaaagtt tggcagatgt agtgaatgaa atgactgatg gaaatggagt
     9241 aaacaaagga tttgattgtt ctgggtttat gccagcagtt aacgaagcct taagactgct
     9301 tagaaaaaaa ggtactttcg tacaagttgg aatctttgct aaaaaattaa atgaaatgga
     9361 ccaagaagca ataatccaaa gagagttaca atatatagga agcaggtctc aaaagccaag
     9421 ttcttggatt acagcattag atttaatggc aaatgggaaa gtaaatacag aagctttagt
     9481 tactagaact gttggtttag acgaatttaa agaaggtatt acagctttaa tgaacgggga
     9541 agaaataaaa gtagcagtaa aatcttaaaa aaacatttgt attattttaa aaaaaattgt
     9601 aaaatctact gtaggataaa aaaatgtaag gtcttaaaaa ataacaggag gaagaaagta
     9661 tgagcactct tagagatcat tctaataata ttagaaaaaa cataataaaa atggttgcag
     9721 aagctaaaag cggacatcca ggtggttcat tatcaatagc tgatatgttg acagtattat
     9781 attttgataa gatgaacgta tgtgcagaaa atccaaaaat ggctgataga gatagatttg
     9841 ttctatcaaa aggtcatgca gcaccagctt tatatgctac attagcagaa aaaggatttt
     9901 tcccagaaga agaattaata acactaagaa aatttggttc taagttacaa ggtcacccag
     9961 acatgaaaaa agtagcagga atcgatatgt ctactggttc tcttggtcaa gggctatctg
    10021 cagcaaatgg tatggcactt gcaggaaaat tagataacaa agactataca gtatatacta
    10081 tattaggtga tggagaaatc caagaaggtc aaatatggga agctgcaatg acagcatcac
    10141 attacaaatt agataattta attgcatttg ttgacttaaa tggattacaa atagatggtt
    10201 ctaatgaaga agtaatgaat gtaagtccag tagatgacaa attcaaatct tttggatgga
    10261 atgtaatagt aataaatgga cattcttttg aagaaatagg aaatgcagtt gatgaagcta
    10321 aaaaagttac aggaaaacca actgtaataa tagctaaaac agtaaaaggt aaaggtgttt
    10381 catttatgga aaataatgct gcatggcatg gaacagctcc aaatgcagaa caagcagagc
    10441 aagcattgaa agatttagaa ggaggtctat aggaatggga atagcaacta gagaagctta
    10501 cggacaagtg ttaaaagaac ttgcagaaaa taaagatata gtagtattag atgcagattt
    10561 aggaaaagct acaaaaagta tatcatttaa agaagttgcc ccagatagat tttttgacat
    10621 ggggatagca gaaggtgaca tgattggtac agcagcaggt cttgctactt gtggaaaaat
    10681 accttttgcg agtacttttg caatatttgc agcaggaaga ggctatgagc aaataagaaa
    10741 ctctgtagca tatccaaatt taaatgtaaa aatagcagct actcatgcag gtgtaacagt
    10801 aggtgaagat ggtggtagcc atcaagctat agaggatata tctctaatga gaggtatacc
    10861 aaatatggtt gtattaaatc cagcagatgc tttagaagca agacaagcta ttcttacatc
    10921 tattgattat aatggacctg tttatataag attaggtaga gctgcaactc ctgatgtaaa
    10981 cagtgaaaat tataaatttg aaataggaaa aggaactgtt ttaagagaag gttctgacat
    11041 aacagtaata gctacaggaa taatggtagc aaaagcctta gaagcagcag aagaacttgc
    11101 taaagaaggt ataaatgtag aagttgttaa tatatctaca ataaaaccat tagatgaagc
    11161 attaataaaa gaaactgcta agaaaactgg aaaagtagtt acagcagagg agcacagtat
    11221 aataggtggt ttaggttcag ctgtatgtga agctttagca gaaactaaag atgtagtagt
    11281 tagaagaatt ggtgttaaag atgtatttgg tcaatcaggt acaccagctg atttattaaa
    11341 acactatggt ttaactacag aagatatagt aaaaaatata aaagagttgt tatagatata
    11401 aatatatcta aataacaaat tgatttgaca taagtcaatt aaagctgtct taaaaataga
    11461 aagaaatttc tacaatggga cagctttatt tttacaatac tttattttta tgataattat
    11521 aaaatagtct gaaagtaatc aaaaaaatta taaaaagagg tgctatttaa tgaaaatatc
    11581 tattggatgt gaccatggtg gttatgaatt aaaggaatat ataaaggaaa atttaataaa
    11641 taaaggtata gaagttgaag atgtgggtac aaattcaaaa gaaagtgtag attttccaat
    11701 atatgcaaaa aaggtaagtc aaaatgttca aagtaaaaaa tctgatttag gcatactttg
    11761 ttgtggaaca ggaataggta tgtctattgc agcaaataag tttaaaggaa tcagagcagc
    11821 tgttgtttct gactgttttt cagctaaggc aactagagaa cacaataata caaatgtact
    11881 ttgtttagga gaaagagtta ttgggaaagg attagcaatg agaattgttg aagaatggtt
    11941 aaattcaagt tataatggag aaagacatgc aagaagatta caaatgattg atgatataga
    12001 aaaagaaaat ttataaatat ataatttata acaaggggga gcgttatgtc tattatttgt
    12061 gcttcaataa tgtgtgcaga ccaaatgaag ttaaaagagg aattagaagc tcttgaagaa
    12121 gcaggtgtaa aattacttca ttgtgatgtt atggatggaa tatttgttaa aaacttggct
    12181 atgggaccag agttattaaa atcaataagt gaaaatacaa caataccttt agacatacac
    12241 ttagccacag aaactccaga taaatatatt gatatgatgt cttatataaa gccaaaatat
    12301 atttcttttc atgtagaatc aagtacaaat gtaaaagctg atatacaaaa gttaagaaat
    12361 tatggtatag agccagtgtt agcaataagc cctcaaacaa gtgtggataa aatagaagaa
    12421 tatataagtt tagttgaggg tatcttagta atgacagtaa atccagggtt tgcaggtcaa
    12481 aagtttaatc tttctgtatt ggataagttg gataaactta cagagatact aaaagattat
    12541 gataatcctc catttataga ggttgatgga aatataaata aggatactat taaacttatg
    12601 aatggtaaaa aagtagatat atatgttgtt ggtacttcag ctttatttaa tgataagcct
    12661 ccaatttcat ataaagataa aatagaagaa ctaaaagaaa gtataaagta accatgtaat
    12721 aaaattataa tatgtttctg tttaatagta aaaatttagg tggaaacaag aaattttata
    12781 atatttaata aaaattttgg ttgagtaagg agaatttttt atgggagacc tatataaact
    12841 acaaaatggt actgatatta gaggaatagc gtatgaaaat gattctaaag aagttaacct
    12901 aactgtagaa gaagtaaaaa aaatagctaa agcgtttcat atttggttaa aagaaaagac
    12961 taagaaagat aaagtaactg ttgccatagg aactgattct aggataacag gaagccaatt
    13021 tagaagtact gttattgaaa ctttaactaa tgacgattgt aatgtaattg attgtgagat
    13081 tgccacaact ccagctatgt ttatgacaac tattatggat ggatacaatt ctgatggggc
    13141 gattatgatt acagcaagtc acttacctta ttactataat ggtttaaagt ttttcacaga
    13201 aagtggaggt ttagaaaaaa ctgatataaa agagatgttg gatatagctg ttaaaaatga
    13261 tagtgtagac tatgaggaag taaataaaaa aggtgaagtt atagtaaaaa atttaataga
    13321 agattattct aatttattga tagataaaat tagaaaaggt gtaaattcaa gtaaaaatta
    13381 tgaaaagcct ttttctgggc ttaagatatt ggttgatgca ggaaatgggg ctggtggatt
    13441 ctttgctgaa aaagttttac acatactggg agcagatacg acaggtagtc agtttttgaa
    13501 cccagatggt atgttcccaa atcatatacc taatcccgaa aacaaagaag caatggaaag
    13561 catatgtaaa gctgtgttag ataataagag cgacctagga ataatctttg atacagatgt
    13621 agacagagct gctattgtag gtaaaaatgg caaaccaata aataaaaatg ctcttatagc
    13681 tgttatatca tcaatagtat tagaagaaca tccaaaaaca gctatagtaa cagattcgat
    13741 tacttctgaa ggacttgcta aatttataaa tgaattacaa ggaagacatc atagatttaa
    13801 aagaggatat aaaaatgtaa taaatgaagc tataagattg aatagtgaag gtgaagagtg
    13861 ccatttagct atagaaacgt ctggacatgc tgctttaaaa gaaaattatt tcttagatga
    13921 tggggcatat ttaatagcta aaattttaat aaaagtagca aagctatcct tggaaggaaa
    13981 gactatagaa gaattgatag aaaaccttga agaagctcaa gaagagaaag aagttagaat
    14041 aggcattaat aaagaagatt ttagaccata tgcagaggat atattaaaag atttagattc
    14101 tcatgtcaag agtattgatg gatggtctgt agcaccaaat aattttgaag gcataagagt
    14161 taattgtgat gaatcaaatg gagatggatg gtttttactt agaatatcac tacatgaacc
    14221 attattagct ttaaatattg aatcaaataa aattggcgga gcagaacaaa tttattctaa
    14281 gttaaaactt ttcttagaaa aatatgattt aaaaggtctt tagggtaaat agaaagaata
    14341 tatttatatt gaagaataaa tgtaaatttg tacctaagat tacaaatatt acaaaattgt
    14401 aatcttaggt aacagagatg taactgatta tggaaacact atgtgatatt atagatacag
    14461 agagatgaaa tatgtgatga attatagaga aatataattt aaaatgtatt aatatcacac
    14521 caaaaaggta taatcaaaaa atatgtaaat ataaacccca acatttagaa gaagacctct
    14581 aaggaggttt ttttcttgtt taaataactt aaaatatatt tttatatcaa tatatcaatt
    14641 aactcttcta aaagtaagca ttaccttaaa taaatcagca tctataatta tttccatgtg
    14701 tgcattttct aattcaatta tattttgagc aattgataaa ccaagaccag agccatctgt
    14761 ttttctagat atatcacccc ttctaaatct ttctattaac tcttctggct ttagatttag
    14821 tttatcattt gaaatatttt taaacgttaa tattatattt tcttccttta taaacatatc
    14881 tatgtagacc ctagtgttag gcatagagta ttttagtata ttgcaaatta gattctcaaa
    14941 aactctccaa gtttttttac catctatcat ggtaaaaaca gcatattcag gtgtttctat
    15001 tataaaatct aaactgctat tttgaatttt gtcttcaaac tctacaatac tttgaattaa
    15061 aagttctctt atatcggttt ttgttatatt aaattctaca tctccactac ttagcttaga
    15121 tgcttcaaat atgtcatcta ttaataattt taatctttgt gattttttat ctaaaatatt
    15181 tatatagtca tttatagtct ctctgtcaaa gtcattattt tttaacatat caacatagct
    15241 taatatagat gtaagtggag tttttaagtc atgagatacg tttgtgataa gctctgcctt
    15301 catcctttca ttttttatat tagcatctac agcagtttca agaccacctg ttatattatt
    15361 tatacttttg gctaaagtag atatttcatc atctcctata acttctaatt tatactttaa
    15421 attgccattt tctatatttt ttatattttt tgatatgtta tttatgtaat taaacttaac
    15481 agataaaaag ttgtatatat aaaaatgaaa aactgtgttt acaagtgtaa ataaaattac
    15541 tatagctatt gcctcagcat aatatatata cataaagaat atcaaaacaa gatattggat
    15601 aatagcttgt acaactatag atattaaaaa tactatagct aattttttat ttatagtttt
    15661 tttattatac atattaagca agtttgaaaa aaataca
//
LOCUS       DAYAOX010000090        15585 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.090, whole genome shotgun sequence.
ACCESSION   DAYAOX010000090 DAYAOX010000000
VERSION     DAYAOX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15585
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.090"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(66..800)
                     /locus_tag="ACGI57_002099"
     CDS             complement(66..800)
                     /locus_tag="ACGI57_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFG9717176.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            925..1641
                     /locus_tag="ACGI57_002100"
     CDS             925..1641
                     /locus_tag="ACGI57_002100"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFG9717177.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1775..2467
                     /locus_tag="ACGI57_002101"
     CDS             1775..2467
                     /locus_tag="ACGI57_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9717178.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2457..3638
                     /locus_tag="ACGI57_002102"
     CDS             2457..3638
                     /locus_tag="ACGI57_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9717179.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            3920..4375
                     /locus_tag="ACGI57_002103"
     CDS             3920..4375
                     /locus_tag="ACGI57_002103"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFG9717180.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4365..5090
                     /locus_tag="ACGI57_002104"
     CDS             4365..5090
                     /locus_tag="ACGI57_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HFG9717181.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5062..7443
                     /locus_tag="ACGI57_002105"
     CDS             5062..7443
                     /locus_tag="ACGI57_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HFG9717182.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7648..8877)
                     /locus_tag="ACGI57_002106"
     CDS             complement(7648..8877)
                     /locus_tag="ACGI57_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFG9717183.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            9478..10392
                     /locus_tag="ACGI57_002107"
     CDS             9478..10392
                     /locus_tag="ACGI57_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9717184.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            10416..11051
                     /locus_tag="ACGI57_002108"
     CDS             10416..11051
                     /locus_tag="ACGI57_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HFG9717185.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            11427..12143
                     /gene="pyrF"
                     /locus_tag="ACGI57_002109"
     CDS             11427..12143
                     /gene="pyrF"
                     /locus_tag="ACGI57_002109"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFG9717186.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            12169..13212
                     /gene="carA"
                     /locus_tag="ACGI57_002110"
     CDS             12169..13212
                     /gene="carA"
                     /locus_tag="ACGI57_002110"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFG9717187.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            13265..>15585
                     /gene="carB"
                     /locus_tag="ACGI57_002111"
     CDS             13265..>15585
                     /gene="carB"
                     /locus_tag="ACGI57_002111"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HFG9717188.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIM"
BASE COUNT         6271 a         1720 c         2811 g         4783 t
ORIGIN      
        1 gtagattata taaagattat ttacaattaa tacaatcttg cttttttatt taaaaatttt
       61 aattattatt taaaattttt tatcttctct ttatcccata taaacaattt ctcagaatta
      121 tttaaagcac caaatacatt tcttttaaaa tcaggcatct ctttatttaa attagcaata
      181 agatttttta tgtactctct cctagtatga ccatttgcag ggcagggatt tgtgataata
      241 ggatagttag aatcttttga aattttctta gtcatatact cttctatgta aaccataggt
      301 cttataactg ttaaatcctg tctgtctagg tatgtctttg gggaaaaaca atttactcta
      361 ccctcataaa acatagacat taaaaatgtc tcaattgcat catctttatg atgaccaaga
      421 gctactttat tacatccaag ttttttagca ttatcgttca aagcacctct tcttaaatta
      481 gcacacaatg cacaaggatt cttttcttgt cttatatcaa atacaatttc ttttatgtca
      541 gtttgaaatt cataaaaagg aacatttatt tctttacaca atttatctag tggagaatta
      601 tcaacacctc cagtatttaa tgttatggct atcaattcaa agttttgtgg agaaaatctt
      661 tgataacttt taagtatatg aagtagagtt aaactatctt ttccaccaga aagtccaacc
      721 gcaattttat cattttcttg tatcatatca aaatcttgta tagccttacg agctttactt
      781 agaagttttt gtatagacat tttatcacca tcctttaaaa atattaaatt taagatttat
      841 ttaagattta cttataaaat acttaagtct tatactctaa aattaacatt atagcaaaca
      901 taacaaaaag gagaagattt aattatgaaa aaatttaaat taataggagc cattatttta
      961 gtagtattta taagtgtatt tatatctaaa gatagattac ttaagaaaga taaagatcca
     1021 atagtagctg ctcaacatga ttcagaacaa gatgttgagg caaattctca aaataaaaat
     1081 cactcaaata gtatatcaaa tatattattg gtaaacaaga caaatggaat tagcaaaaac
     1141 tatactcctg aaaatattac taaggtaaac ataccttttg ttgaagaagc aacagaggaa
     1201 gaaaaacaaa tggcaggaga acctgcaaag gcagttgaag atttagttaa gcaagctaat
     1261 tcagaaggaa tacagttttt agggtcttca gcatacagat cttatgatac tcaactggat
     1321 acttatacaa gaagggtaaa atctcagggg cgagaaaagg cagatgcgta tgtagcaaag
     1381 cctggttaca gtgagcacca aactggactt tgtattgatt taacaaatcc agaaagatgg
     1441 tttgttggaa gtacaaaaga agctaaatgg ttggcagaaa atgctcacaa gtttggattt
     1501 ataataagat atcctgaagg taaagaagat ataacaggta cagcatatga accatggcat
     1561 attagatatg ttggtaagga tgctgccgag gaaatttatt caaaaggatt aactttagaa
     1621 gaatatttac aaaataaata atataaatgt gtaaataatt tgtattatat tggaaattta
     1681 gaaattaata aatttataga ttacactaac taaaatatag tataatttta tatgaataaa
     1741 gctatataca aagattaagt taggatgtgt aaaaatgaat aatacagttc ttgttgtgga
     1801 tgatgaaaag gaaataagag accttataga gatatatctt ttaaatagtg gatataatgt
     1861 aattaaagct gaagatggaa aagaggctct agatatttta aaaaaggaaa atattcattt
     1921 aatgatattg gatataatga tgcctaaaat ggatgggata gaagtttgta gaaaagttag
     1981 agaaagtttg aatataccaa tacttatgtt aagtgcaaaa tcagaagaca tggataaaat
     2041 acaagggata atgactggtg cagatgatta tttgacaaaa ccatttaacc cacttgaact
     2101 tactgtgaga gtgaagtcgc tgattagaag agcatattat tttagtggtg ctaataatga
     2161 agataaagat gtcataaagg ttggagtagt aattatagat aaggaaaaac atagtgtaac
     2221 agtggctgga aatgaaataa ttttgacatc aagagaattt gatatattat atttactagc
     2281 aaataataaa ggcagagtat atagtacaga agaaatattt gaaaaggtat ggaaagagaa
     2341 atattaccaa tctaataata ctgtaatggt tcatatgagc agaataaggg ataaaattga
     2401 aaaatataca gatggagaaa aaataattca tactgtatgg ggagtaggat ataaaattga
     2461 aaaataaaag tttattaaat catatgtttc gacccatatt agatcttata ataagctgtg
     2521 ctttaacttc aatgcttatt ttaacaagta tgtatataat aaaatcatta tatgtcaata
     2581 gtgatatgtc tgtagttaat caacttgtat atcaagcaag agatttcaga agtcttatag
     2641 ggtatgattt attttatcca tttttattta caatcacatc attagcaatt tattccatga
     2701 tatcgtacaa gagaaataag caaattgtag attttataag aaagactaat agaaaaaatc
     2761 aaaaattaga aatcattaaa agtgctgtaa atttaatgga tgaaggtaac ttagaaaaaa
     2821 gtatagatat tgagtatgat ttagatatat taaaggataa agaaaaggat gatattgatg
     2881 aactagcaca taaaatcaat aatatagtcc atcaacttag aaatataact attgaagaga
     2941 gacaagctca gcagacaaaa acagatttaa taacaaatgt gtcacatgac ttaagaacac
     3001 ctttgacatc cataatgggt tatttaggag tgatagaaga aggtaagtat aaagatgaag
     3061 ttcagatgat gtattatgtt gatattgcat atgaaaaatc gaaaaattta aatgttctta
     3121 taaatgattt gtttgaattg actaaaatgc aaaataatac aataaaatta aacaaaatag
     3181 aaataaattt agtagagtta ttaagtcaag tagtatctca atttgaaatt tattttaaac
     3241 aagaatttat ggttagtaga attaatttta atgaagaaaa attaattgta aaagctgatc
     3301 caaataaatt ggtaagagca tttgaaaatc ttataactaa tgctgtgaaa tatggaaaag
     3361 atgggcatta tgtagatata gtcacagaga aaaaggaaga tatggcggtt gtaaaagtga
     3421 taaattatgg ggaaccaatt ccagtgttag atttaccaaa tatatttgat agattttata
     3481 gagttgaaaa atcgagaaat agaaatgttg gaggttctgg attgggtctt gcgattacaa
     3541 aaaatattgt aaatttacat gatggagaaa ttactgtttc aagcgataaa tatcaaactg
     3601 tgtttgaagt acaactgcca ataatacaat cagcttagtt ataaaataaa agtacgatta
     3661 tgtgatataa atattataac aataaagtgt ttacaaaaat aaaaaagtaa tatataattt
     3721 aagtcagttt aataagatac ttatcaagag ttaggggaga gagttagctc agtgatccta
     3781 taccaacctt ttatcaggtg gtcctgctaa caacgatgag atgacttgaa ataatatact
     3841 cttcttatca ataatagata ggaagagttt tttttagaat taatgtaaca aattttaaag
     3901 tataatggag gattaaataa tggaaaaagt agaaagtttt aagctagacc atacaaaagt
     3961 aaaagctcct tttgtaagaa agtgtagtgt acttgatgga gtaaagggag ataaagtaac
     4021 taaatttgac ttgagatttt tacagccaaa tgtagaaagt tttggaactg ctgctatgca
     4081 cggtttagag catcttttag ctacttattt aagagataca ttagatggtg taatagactt
     4141 atctccaatg ggatgtagaa caggatttta tttaatacta tggggagatg tagatgccaa
     4201 aacagttaag attggtttag aagaagcatt aaaaaaagtt cttgaatcag ataaaatgcc
     4261 agcagctaca gctatagaat gtggaaacta cagagactta tctttatttg gggcaaagga
     4321 atatgcaaaa gatgttttag ataaaggatt ttctttaaat atatatggag aataatttaa
     4381 ttggaagaga agatataagc ataaatcaaa gtgaagtttt aagatattta caatatagag
     4441 gtcaagagat agataaagac cttaaatata ctattgatga atgtgtagct ataactaaaa
     4501 ataaaataaa tcctagatat ttatctagga tttatcctat taaaataaat aaagataatg
     4561 ttgtagaatt agagggaaca aatttaacat tagagagtaa agatgtatat gaacttttaa
     4621 aagaatgtaa tgagtgtgta attatggcag ctactatagg gatagatata gaaaaagaaa
     4681 taagacgaca ctcttattca aatttaacaa aaggactaat aattgattca tgtggaacaa
     4741 cagcaataga agaagtgtgt gacattgttc aaaataattt agaaaaggtt tttttaaagc
     4801 aagataaatt tataactatg cgatatagcc caggatatgg agatttacca atagaaaaaa
     4861 atatagatat attaaacgtt ttgaatgctc aaaaacaaat aggtctaaca attacttcta
     4921 atggaattat gataccgaga aagtcagtag tggctattat aggtatttct aatacaagaa
     4981 taaataaatc taagaaagaa aagtcgtgtg aaaattgtaa aaactataga aactgtacat
     5041 atagaaaagg agcgaactct tgtggaaata agacaatatc tgaaaaataa tatattaata
     5101 tttgatggtg caatgggaac aatgcttcaa caaaaagggt taaagttagg tgaaaatcca
     5161 gaagtatttg gtcttcaaaa tccagataaa cttatagaaa tacatacagc atacttagag
     5221 gcaggttcga atgtaattct tacaaatact tttggatgta atgaactaaa attagatagt
     5281 aagtatactg tagaagaagt gattgacaat gctgttttgg tggctagaaa agctatagaa
     5341 aatgtagata atactaaacc aagatatgta gctttagata ttggtcctat aggagaaatg
     5401 ctagagccta tgggtacttt aagttttgat aaagcatatg aaatatttaa aagacaagta
     5461 ctacaaggcg ttaaatctgg agttgatgta atagtaatag agactatgat ggatttatat
     5521 gaagctaaag tagctgtatt agcagcaaaa gaaaactctg atttacctat tttctgtact
     5581 atgacatttg atgaaggtgg aagaagtttt actggatgta tgccagaatg tatggtggct
     5641 actatagaag gtctaggtgt agatgcaata ggagttaatt gttcattagg acctaaacaa
     5701 cttttaccaa tagttgaaaa aatagcatca agagctacag tccctgttat ggttcaagca
     5761 aatgcaggtc ttccaaatat agttgatgga gaggctatat atgatgttga tgcaaaagaa
     5821 ttttttgaag gggttaaaaa gttcgttgaa gttggtgcta ctataatagg tggatgttgt
     5881 ggtacgaatc caagttttat aaaagaaata tctgagaata taaattctgt tacaaaggga
     5941 tgtatcgaga aaattgataa atgtgtagtc tgttcaccat caaaatttgt ggaagtacaa
     6001 tcgcctacag ttgttggaga aagattgaat cccacaggta gaaaatctct acaagaagca
     6061 ttaaaaaatg aaaatgttga ttatgctata aatttaggtt tagaacaagt aaatgctgga
     6121 gcacaaatac ttggagtaaa tgttggtctt ccagagattg atgaaaaaaa actaatgcca
     6181 aaattaataa gagagattca agcagtagta gatacacctt tacaagttga ttcttccaat
     6241 gtagaggcct tagagcaagg acttagatat tataatggta gaacaatagt aaactctgta
     6301 aatggcaaag aggagtcttt agaaagtata ttaccaatag ttaaaaagta tggttcatgt
     6361 gtggttggac ttacacttga tgaaaagggg atacctaaaa aggctgaaga aagatttgaa
     6421 atagcaaaaa ggatagttaa tagagcagtt tcttatggga taaaaccaaa agatatattt
     6481 atagactgtt tatcattaac tgtttcagca caacaagaag aagcaataga aactataaaa
     6541 gctataacaa tggtaaagac actaggagta aaaactatat taggtgtttc aaatatttca
     6601 tttggtcttc caaataggaa agcacttaat gcttcttttc taacattagc tttaggggca
     6661 gggcttgatt tagcaattat aaatccaaat gaatatagta tgatggaagc tatcaattct
     6721 tttaaaatat taaataacac agataaagga tgtataaact atataaatca atatagcaat
     6781 ataaataatt caaaaaaatc ggattcatct acaaagattg ataaagattt gcccttggat
     6841 attttagttg aaagaggatt aaaagatgaa gcaaaaaatg taacactgaa tcttttaaaa
     6901 gagaaggatg aaaattatat attagatgag attttaattc ctgctcttga taaagtagga
     6961 aaaagatatg atagtggaga tatattttta ccacaattga ttcaatcagc agaaacggta
     7021 aaagtttcat taaatacaat aaaggaaact ttattaagta aaagtagcaa taatgtatcc
     7081 aaaggaaaaa taatagttgc tacagtaaaa ggtgatatcc atgatattgg gaaaaatata
     7141 gtaaaaataa tgctagaaaa ttatggatat gaagttatag atttaggtaa agatgtccct
     7201 attgaagaag tagtggaagt tgctaaaaag agaaatataa aattagtagg attaagtgca
     7261 cttatgacta cgactgttca aagtatgaaa gatacaattc aggcactgag agataatgag
     7321 ataaatgcaa aggtatttgt aggaggtgca gttttgacag aagaatacgc tgaagaaatg
     7381 ggtgctgatt attattcaaa ggatgcaaag tctgctgttg aaatagcaaa gttaaatttt
     7441 tagcatataa attaaaataa aggttttact aaataggata ttttatatat aatataaata
     7501 taagttttag atacttatgt ttaaaacaat aggaattaat ctataataat gagattaatt
     7561 aatataaaaa atatttatca tcaacattaa aaagggctgg gaagtctggt ataacttcca
     7621 tcagcccttt ttagttaaaa tcatatgtta aaaagcccaa ttaccatttt taaaaatctg
     7681 tatttgcttt ccatcataag tctcaccaat aatatccata tcagcagaac caatcataaa
     7741 atctacatgt gttaaactgt catttacacc atgttcatct aattcttcat cttttaaatt
     7801 gtttccacct tccagacaag tcttataagc agaaccaatt gcaaagtggc aagaagcatt
     7861 ttcatcatat agagtattat aaaaaattat atccgtattg gatattggtg agttgtatgg
     7921 aacaagagca acttctccta aataatgaga gccttcatcc gtttcaataa gtttttctag
     7981 tgtatctagt cccttttcag cagagaaatc tattatttta ccatctttaa atgttaagaa
     8041 gaaattgtca attatgtttc caccataaac aagaggtttt gtactataaa cagttccatt
     8101 tactttaaac ttatgtggca ttccaaaaat ctcttcagtt ggaatattcg gattaaaact
     8161 tattccatta gggtctttgg atgctccact tagccaaaca tgaccctttg gcaagtctag
     8221 agtcaaatca gtttttgagg atttatattt taaagtttta aagttatttt tatttagaaa
     8281 gtccatttta gatttaagat tttcattatg ttcttcccat gcttttatag ggtcctgacc
     8341 atctactctt gaacatttaa atatagcatc ccacagtttt tctattgctt tatcttctga
     8401 taaatttgga aacacttttt ttgcccaatc ttttgttggc acagagacta tactccattt
     8461 acatttatca gttagagtgt aagaacgcca ttcttttaaa gcttttccag atgccttctg
     8521 aaaatttgca actctttcag ggtcaacatc ttttaataaa tctggatttt gggcatagac
     8581 tgataaaaat gccccacctt cttttgctat atctacatat tgttctgctg tccatttggg
     8641 gaactcatga aatgcttcat ctggagcata taagtattta ataagagtac tttcttcatc
     8701 agaccactct atataaacat ttttagcacc acatttatat gcttctttaa gtgcatttct
     8761 tgcaaaatca gcacattcta ttggtgactt tataagtaga atttgacctg gctgtatgtt
     8821 aactccaact tttattgcta attcagcgta tttttctaaa tttctattta aattcatatt
     8881 tatcctccta tatttttaat attgaataac ttataaagta ttataacatt acttatgttt
     8941 ttaatattga acaacttata aaatattata actttactta gattttgggt catattcaaa
     9001 atatttatac tttatagatt gagaaataaa aaaaatattt taattaaaac aaattatgag
     9061 gataacaaac ttatttaagt agtatgtttt ttgttgataa taatattaaa gtagaaataa
     9121 aaatactttt ttagtgaaaa gctatacaag aataccgaaa aaaacttata atataataat
     9181 gagatagata tttataaatg aaaaaaaatt tttctggcaa agcttaatta aaaaggtaat
     9241 agctagtgtt gttatttaaa ttagcataga aaatcaaata aaaaaattat gctaatgtat
     9301 agacattgat aaggtagaat ttaagccttt tgaatgctat attaaactag agtagatatt
     9361 cttatgaatt tcaaaatagg gcaggaggaa aaaggaaata aaactataaa aaatataaag
     9421 tatttatata aatttataga gtttatacgt cagaagattt cagttaggag ggcataaatg
     9481 gagctttatg aagccatttt ttatagaaaa tctgtaagaa atttttccag taaaaagata
     9541 aaaaagccat tgatggaaga aataaaaaga agttgtaaaa atttagaata cttaaatgaa
     9601 gatttaaata ttaaagcaca tgttgttgat agaggtcata taatacattt tttgatgggg
     9661 aaggaatgta aagtaaaggc acctcattat atagtagtta catcagacaa aggtgaggac
     9721 tatttacaga atataggatt tgctacagaa ggtgtagttt tgcgccttac aacattggga
     9781 atagctactt gctggttgga aggaaactta aagagagaag atatcttgga atttgtagat
     9841 attgggaata agaattttga agaagatgag gaagatgatt tagattatct agctggaata
     9901 aaaacagaag aagatgatga aaacaaaata gaaaatcctt atataataat agcatttgga
     9961 tatccagcaa aaaatgaaga gttgtttagg acgagatatg ctagacctga tagaaagcca
    10021 gtaaaagata tgataaataa gataaatagg aatagaaagt ggaaagatat cttagaattg
    10081 acaagaagag ctccatctgt aaaaaattct caaccgtggt atttccataa ggatgaacga
    10141 ggtcttcatt tatttgaaaa gagacctaag aaacactgtg aagatatgaa taaggttagc
    10201 ttaggtgtag ccttaagaca ttttgatata gcttgtataa aaaataaaat agatgtttca
    10261 tatgaaaaac ttcctataag gaataaaata ggaaaatcat attttataac tgtagtcgag
    10321 catgtgaaac cagaagaaga aacccaagaa gaaaatgtaa ctctagaaaa agaagaatcg
    10381 caagatgaat aaaattttgg aaggggatat tggatatgta taagtgtgga aaatgtaatc
    10441 ttagaggctg ttctaatgga aatatggata aattaatgaa gatatgtcca actagagaaa
    10501 ttgaactaca acaaaaagca aagcaacttt atgaagaaga agagaataga aaaatagctt
    10561 ataatgctgg attagttgaa gcagaggggt attgtcaata tactagacta aaagagatta
    10621 tagagtttgc taaaaagtgt ggttataaaa agattggcat agcattttgt attggactaa
    10681 aaagagaaat gcaaacagtg caaaaaatat ttgaacatca tgggtttgaa gtattttctg
    10741 ttatatgtaa aaatggggca atgcctaaga gtacaattgg actagaagca tctcaaacaa
    10801 tatcaaagtg ttcagatgag gtaatgtgca atccaatagg acaagcttta tttttaaatg
    10861 aagctaaggt tgaccttaat gtaattcttg gattatgtgt tggtcacgat actttagcca
    10921 tgaaatatat ggaagctcct ataacaactt tagttgtcaa agatagagta actgggcata
    10981 atccagttgc tgcaatatat aatgcagaat catattttga gaaaaaattg tttgataaag
    11041 atgaagaata attcaaagac ttattgacat aaaaagcaat tagtggttta attataaaaa
    11101 agataaaact ttaaattatc ctatagatac ataaagttat ataaataaag aaaaatatta
    11161 cagctaaatt ccttaaagta gtccagagag actaaagggt tatagataat gttagtatat
    11221 aacctctagt aaatcttcta gaggtttttg atgagtataa aataaaaatt taaatataat
    11281 aaagtctttt aaactagtcc agagagactg ggaaagatat ctcatagcta atgaatagag
    11341 ttatgtatag ataaaatcct tatgcctctt ttggtgtgag gattttttta tgattattta
    11401 ggctttatta aaaaggagat gtaaaaatga taaatggtaa agaaaaattg atagttgcaa
    11461 ttgatactga cgaatttgat aaagctaaag aactaataga cagattagaa gatagtgtag
    11521 acatatttaa ggtagggcta gagcaatacg ttgccacaaa aggtaaaaca atagattatc
    11581 ttaaagaaaa agggaaaaaa atatttttag accttaaatt ccatgatata ccaaatacta
    11641 tgaaaagtgc agtgaaagct gcagtgagag ataatgtttg gttaatgaca atacatgtat
    11701 cagatatgga aggaatgaga caatgtgcac taattgctag ggaagaagca gaaagattaa
    11761 atatagaaaa gccattagta gttggagtaa cagttttaac ttcattaagc aaccaagatt
    11821 tacaggacat aggatgtaat atgacaacag aagagttagc tataaagaga gctaaactgg
    11881 caaaagaatc aggaatagat ggggttgttt gttcagcgca agaagtagat aaaatagtag
    11941 aagcatgtgg aactgatttt ataacagttt gcccaggaat aagacctaaa tcagcagagg
    12001 ttggagacca aaaaagagtt gtaactccat cagatgcaat aaaaaaaggt gctcattatt
    12061 tagttgttgg aagacctata acaaaagcag aaaatccaag agaatcagct attaatattg
    12121 taagagaaat agaaaatgct tagataaaat atttgggagg tagcttacat gaaggggaaa
    12181 ttaattttag aagatggaac agtatttgaa ggaaaagcat ttggatatct aaaggaagct
    12241 attggagaag ttgtatttaa tacttcaatg gctgggtatg gagaactatt gacagatgca
    12301 acttattatg gacaaatagt tactatgact tatccactta taggaaacta tggaataaat
    12361 ttagaagatg ttgaatcaaa taaggtgcaa gtaaaaggac ttattgtaag agaaaaaagt
    12421 gataacccaa ataattttag atgtgaaatg gatatagaca catatctaaa gcaaaataag
    12481 gtaataggac ttgaaggaat agatacaaga gcattaacta aaattattag aaacaatgga
    12541 acgaagagag gaattataac tttagaaaat gtatcattac aagatgttca gtctaaatta
    12601 gaagagcatt gtaacaaaga ttcagtaaaa aatgttacaa gaaaagaaat tgaaaatata
    12661 aaaggtactg gagctaaagt tgtagttata gactttggtg ttaaaaatag tgtactagag
    12721 tctcttaagg cttgtaactg tgatataact atagtaccag caatgactac agtagaagaa
    12781 attttaaata taaatcctga cttaatctta ttatctaatg gcccaggaaa tccagaggat
    12841 ataaatgata tagttgaaaa tataaaaaaa ttaataggca aaaagccatt gacaggaata
    12901 ggtttaggac atcaaatttt agcattggca ttaggaggaa agacttcaaa gcttgtattt
    12961 ggacatagag gaggtaatca cccagttaaa tgtttagaaa ctggaaaggt atttataact
    13021 tctcaaaatc atggatataa tgtttcagaa atgccagaaa atacagaagt aactcacata
    13081 aatttaaatg acaatactat agaaggaatg agacataagg aattaccaat atatagtgtt
    13141 caatacattc cagaatacac aacaaaagaa gatttagatt gtatatttaa taaattctta
    13201 gcattaatat agatttagta ttatttacag atagtaaaaa ccatttagat aaggagaaaa
    13261 atttatgcct aaattagata gtataaagaa aactttagta ttagggtcag gaccaataat
    13321 aataggtcaa gctgcagagt ttgactattc aggaacacaa gcttgccagt ctttgaaaga
    13381 ggaaggaatt gaagttgtat taataaatag taatccagcc actataatga ctgataaaga
    13441 agtggcagat aaaatatata tagaaccatt aacaatagaa tttatagaaa aaataataga
    13501 aaaagaaaga ccagacagtt tacttgcagg tatgggtgga caaacagggc taaacctagc
    13561 agttgaactt tatgaaaaag gcatactaga taaatatgga gtaaagataa taggtacttc
    13621 agtagaatct atcaaaaaag gtgaagatag agatatattt agagaagtaa tgaaagaaat
    13681 aaatcagcct gtaatagtta gtgatatagt tacagattta caagcaggtc ttgattatgc
    13741 acttacaatt ggttatccag tagttgtaag accagcatac acactaggtg gaactggtgg
    13801 gggtatagct gataatgagg aagaattaag agaaatactt tcacatggtc tacaattaag
    13861 tcctgttgga caagtattaa ttgaaaagag tataaaaggt tggaaagaaa tagaatatga
    13921 ggtaataaga gattcaaagg gaaattgtat agttgtatgt aacatggaaa atatagaccc
    13981 agtcggagtt catacaggag acagtatagt tgttgctcca actcaaactt taagtaacaa
    14041 agaatgtgca atgcttaaaa aagcatcttt agatatatta aatgcagtag aagttcaagg
    14101 tggttgtaac gttcaatttg cacttaatcc acatagtttt gaatatgcag ttatagagat
    14161 aaatccaaga gttagtaggt catcagcttt agcatcaaaa gctacaggat atccaatagc
    14221 taaagtttcg tcaaaaatag ctttaggtta tactttagat gagatagaaa atgcagtaac
    14281 taaaaaaact tatgcttgtt ttgaaccaac tatagattat gtggtggcaa aaataccaaa
    14341 atggcctttt gataaattta aaaaagcaaa tagaaaatta ggaactaaga tgatggcaac
    14401 tggtgaaatt atgagtatag gaagtaactt tgaggctgct attcttaaag ggataagatc
    14461 acttgaaaca ggaaaatatt cattagttca cacaccatct gaggagagaa gcattgagga
    14521 attaaagaaa agagttgtag tacctgatga tgagagacta tttgatttag ctgaaatgat
    14581 aagaagaggc tataaagtag aaatgataga acaaattaca ggtatagata aatggtttat
    14641 aaataaattt aaatggatag tagagcaaga agaaaaatta aaagttatga aaattgaaga
    14701 tttaactaaa gactatcttc ttgaattaaa gaaaaaggga ttctctgata aaggtatatc
    14761 tgacctaatg aagataagtc cagaaaaatt atatgaattg agaagtctat acaatataaa
    14821 accagcatat aaaatggttg atacttgtgc aggtgagata gatgctatat ctccttacta
    14881 ttattcaact tatgagcaat atgatgaggt agttgttagt gataagaaaa aggtgatagt
    14941 attaggttct ggtccaataa gaataggtca aggtatagaa tttgactatt gttcagttca
    15001 ctgtgtaaaa tctttaagaa aaatggacat agaaactata atcataaaca ataaccctga
    15061 aactgtaagt acagactttg atacatcaga taagctgtat tttgaaccat taacagaaga
    15121 agaagtttta agtataatag aaaaagaaaa gccagaaggt gtaatactac agtttggtgg
    15181 acaaacagct ataaaattgg ctaaattttt acatgaaaaa aatataccta tattgggaac
    15241 tgattttgat gatatagatg cagcagaaga tagagaaaaa tttgatgatt tactagagag
    15301 actagatata aatagaccaa aaggaaaggg tgtatggtca cttaacgaag gtattgaagt
    15361 tgctaatgag ttaggttatc cagtattagt tagaccatct tatgtacttg gtggtcaagg
    15421 tatggaaata acttataacg aagaaaaatt atctcaatac ctacaagatg catttgatag
    15481 agaccataaa aatccagtac ttatagataa atatctaact ggtagagaaa tagaagtaga
    15541 tgctatatgt gataaggaag atatattgat acctggtata atgga
//
LOCUS       DAYAOX010000091        15073 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.091, whole genome shotgun sequence.
ACCESSION   DAYAOX010000091 DAYAOX010000000
VERSION     DAYAOX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.091"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            695..3337
                     /gene="adhE"
                     /locus_tag="ACGI57_002112"
                     /gene_synonym="adhC"
     CDS             695..3337
                     /gene="adhE"
                     /locus_tag="ACGI57_002112"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFG9717189.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3697..4038)
                     /locus_tag="ACGI57_002113"
     CDS             complement(3697..4038)
                     /locus_tag="ACGI57_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717190.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(4065..4736)
                     /locus_tag="ACGI57_002114"
     CDS             complement(4065..4736)
                     /locus_tag="ACGI57_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717191.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4748..7324)
                     /locus_tag="ACGI57_002115"
     CDS             complement(4748..7324)
                     /locus_tag="ACGI57_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717192.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            7532..8467
                     /locus_tag="ACGI57_002116"
     CDS             7532..8467
                     /locus_tag="ACGI57_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFG9717193.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8486..9223
                     /locus_tag="ACGI57_002117"
     CDS             8486..9223
                     /locus_tag="ACGI57_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9717194.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLDIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(9408..14465)
                     /locus_tag="ACGI57_002118"
     CDS             complement(9408..14465)
                     /locus_tag="ACGI57_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFG9717195.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(14490..14909)
                     /locus_tag="ACGI57_002119"
     CDS             complement(14490..14909)
                     /locus_tag="ACGI57_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717196.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
BASE COUNT         5280 a         2111 c         2121 g         5561 t
ORIGIN      
        1 aaatatcgaa ttaaatttac ttttatttaa aataaattga ttaaatctac ctgggaaaaa
       61 attaattaat atgttaataa attaaaaatt tataaatatt ttatactgta aaaaattttg
      121 tatatatatt atcattaaaa aattaacggt cttaatataa aaattattta tctatatgaa
      181 aagcataatt attacatatt gacttaaaaa taacttatag agattagaat aattttagtt
      241 tatatatttt ttaaaactat acagttgtta taaaaagaaa aattgcatac aagtcaaaaa
      301 taagtgttat tttataaaaa tacgacaaaa attttattta ctacttatac aaataacatg
      361 caaataacat ataaaattta ataagtaaaa aaaatgaaag atataaatta tcattttagt
      421 attttattta gtcgaagtaa attattcaaa tgaaatatat ctcatttaga taaaaaattt
      481 tagtaaaagt aaaaaaaata atatatttgt taaaaaataa attgacaaaa tattcagaaa
      541 aaaacttctt gacctaagag agtaaaataa ataaaatcta aggtatagag atgaaatcta
      601 taaaattatt ttaagtataa gttaaaaata tagatgatgc atttaagatt tataaaaatt
      661 agagatgcat aattatttta gggaggtttt tagtatggag aaaaaagaaa aggtagtaga
      721 aaaatcaaat gttgaagtat gtagtcctga aattgtcaac agtgttgaaa cattaagaat
      781 gagattagaa gaaattagat tggcacaaaa agaatttgca acatttacgc aagaacaggt
      841 agataagatt ttcttagctg cttcaactgc agctaatcaa caaagaatac ctctagcaaa
      901 aatggcagtg gaagagactg gtatgggtat tgttgaagat aaggttataa aaaaccattt
      961 tgcctcagaa tatatctata atgcttataa agatactaaa acttgtggtg tcatagaaaa
     1021 agatgaagca tttggtttta caagaattgc tgagccagta ggtgttttat cagcagttat
     1081 accaacaact aatcctacat caactgcaat attcaaatcc ctaatagcac taaaaactag
     1141 aaatggtata attttctctc ctcatccaag agctaaaaac tgtactatag aagcagctag
     1201 agttgtacat gatgcagcag ttaaggcagg agcaccaaaa ggacttatag gttgggtaga
     1261 tgttccttcg attgaattaa ctaatgttgt catggcagag gcagacctta tattagctac
     1321 tggtggacct ggaatggtta aatctgcata ctcttcaggt aaaccagctg tcggagttgg
     1381 gcctggaaat gtacctgcaa taatagatga gagtgcagat ataaaaatgg ctgtaagttc
     1441 aatcttagta tcaaaatcat tcgataatgg tatgatatgt gcttccgagc aagctgtaat
     1501 agtaccagaa aaaatatatg aagaagttaa aaaagaattt aaatatcgtg gagcacattt
     1561 cctaaataaa gaagaaactg aaaaagtagg aaaagtagta ataattgatg gttcattaaa
     1621 tgccagaata gttggacaac cagcacatgt aatagccaaa atggcggatg tagaagttcc
     1681 aaaaacagct agaataataa ttggtgaagt agaatctgtt gaattaaatg aaccttttgc
     1741 acatgaaaaa ctttctcctg tacttgcaat gtacaagtct aaaagttttg aagatgcagt
     1801 tgctaaagca gaaaaattag ttgcagatgg aggctatggt catacatctt cattatatgc
     1861 agactctata aatcatccag agagagtaga aaagtttgta aatgctatga agacttgtag
     1921 agtattagtt aatactccat catcacaagg tggtataggt gatttatata actttaaatt
     1981 ggcaccgtct cttactcttg gttgtggttc ttggggtgga aactcagtat ctgaaaacgt
     2041 tggagtaaaa catttgctta atatcaagac agtagctgaa aggagagaga atatgctttg
     2101 gttcagagca ccagaaaagg tttatttcaa aaaaggatgt ttaggtgtag cagcaagaga
     2161 atttaaggat gttatggata aaaagaaagc atttatagta acagattctt tcctatataa
     2221 taatggatat actaagaaat taactgattt attagatgag atgggaatta agcatactac
     2281 attctttgat gtagctccag acccaacatt agcttgtgca agagaaggag ctaaagctat
     2341 ggctgatttc caaccagatt taataatagc tgtaggtgga ggaagtgcaa tggatgctgg
     2401 taagataatg tgggtaatgt atgagcatcc agaagtagat ttccaagact tagctatgag
     2461 atttatggat ataagaaaaa gagtatatgt atttcctaag atgggtgaaa aagcgtattt
     2521 tgctgctatc ccaacttcag caggaactgg ttcagaagta actccatttg cagttataac
     2581 tgaccaagat tcaggtgtaa agtacccatt agcagattat gaattaatgc caaacatggc
     2641 tataattgat gctgatatga tgatggagat gccaccaaga ttaacagcag catcaggtgt
     2701 tgatgcttta acacatgcat tagaagcata tgtatctatg ttaagaactg aaccagctga
     2761 cggtctagca ctacaagcag gtaaaataat atttgaatat ttacctcgtg catataaaaa
     2821 tggaaagaat gataaagaag caagagaaaa aatggcaatg gcatctacta tggcaggaat
     2881 gtcttttgca aatgccttct taggaatatg tcactcttta gcacataaat taggtgcatt
     2941 ccaccatgtt caacacggtg ttgcaaatgc attattaata aatgaagtaa tcaagttcaa
     3001 ttgtgcagaa gctccaaata agatgggtgc attctcacaa tacagatatc ctgattgcat
     3061 acaaagatat gctgaatttg cttcttttgc aggtattaaa ggaagtacag accaagaaaa
     3121 agtagataat cttataaaag caatagatga actaaaggca aaagtaggtc ttcctaagac
     3181 tataaaagag gctggagtag aagaatctaa attcttagaa agattggatg ctatggttga
     3241 acaagctttt gatgaccaat gtacaggtgc taatcctaga tacccattga tgagtgaatt
     3301 aaaagagata tatctaaaag tttattatgg taaataatat ctattgtgca acataggttc
     3361 tattgggttt gtatataaat tattagattt gaatataaat agagttttat tagagacacg
     3421 tagaagtaac tataaataat agattataat ttaaattcta aatggaaatg ataaaaagtc
     3481 agtaatttaa tggtaatttc gaatatgtgt atttaatcaa ttaactttaa aattagtcgg
     3541 aaaatttttt tattacatat aattttaaag cataatatat aaaaaacttt atgaaaaaat
     3601 tataaaaata ttcagaaaat tctattgact aatttattta tcatgatata ataagtttag
     3661 aaaagaaaaa aattggggcc tattaaggcc cccttactat tttttgatag atttatttaa
     3721 aattactttc tcattttgat tttttatatt tattatagag tcagtataat ccacttcaaa
     3781 gttggcaaaa gcagagaata aatctatatc tcttaacaaa tgaattgtca tacattgatt
     3841 gttcttattt agccttagaa tcaatgctct tacaaatgga aattgtattc tagaattaaa
     3901 taagttagaa ccatcagtat ctatatctaa gctacgataa ttaatatcta taaatatatc
     3961 tacatcttca ttttcttctc ttattattct gtagtcaaaa tccaagttta aatttaaatt
     4021 acctaaaatc aataacattt aaatcctctt ttctataaat agttttagag tgttaattta
     4081 agctctccat ctattataga tataattcta tctgcttttc tagcaatatt ttcattatgt
     4141 gttattatta taagtgtttg gttatatttt tttatagaac tttttagtaa atccatgact
     4201 tcttctgtag ttttactgtc caaatttcct gttggttcat ctgctaggat tatacttggt
     4261 tttgcagata aagcacgtgc aatagcaact ctttgctgtt gaccaccaga taattggttg
     4321 ggaagatgat ttttcctttc attaagaccc aataaattta taaggtcatc aatatattct
     4381 ttatctattt ttttattgtc taactttagt ggtaattcta tattttcctt tacatttaat
     4441 acaggaatta agttatactg ttgaaagaca aatcctacct tttgtcttct atatcttgct
     4501 aattctttat ctgaaagaca acatatatct ttaccatcta cctttatagt accattactt
     4561 ggtctttcaa gaccacctac taaatgtaga aatgtacttt tcccacttcc acttggtcca
     4621 attatagcaa caaattcgcc tttatttatt tttaaatttg cattctttag tgcatgtaca
     4681 gatgtttcct tttttccata aatcttacct aagttaatag tttccaatat aggcataatt
     4741 attcctccta ttctgtaatg tttatagctt caactatact aattttattt atctttcttg
     4801 atggaatata tgtagctaga atacctacaa ttatatttgc tacaacaact agtatatata
     4861 ttttgtaatc tattgaaaat cccaatccat atgatacaaa gccctgcata taatacattt
     4921 taaattgtat tataattcct gacacgatag taattatact tgagagtaca ccatataata
     4981 ttccttcata aagtatcata tttttaaagc ctctctcaga gataccaata gctcttaaca
     5041 ttccaaattc actagttctt gaagttaagt tataacttat gttgtttatt atgttaagta
     5101 cactgattat gaacattatt gctactatac cataactata aatcatagtt ctagatgtca
     5161 tcttatcaga attttctttt tctaccatca tatctactgt tgtagttcca ggaacttcac
     5221 ttccaatttt acctaaaagg ttatttattt ttttcttgtt tgcattttca ttaaggtctg
     5281 cataaactaa gttatactta tctacaccag ttaatttttt taggtattcg ctactcgtta
     5341 ttacatctac accatcatct ccagagtaca taccattatc tgcaaaagga taacttataa
     5401 tagctccaac tttaaattca tattctaagt attcataatt atcttttaga ctttcataaa
     5461 gacctgtatc catcccaccc tttggatatt tgacttttat agtatcacct acttttatat
     5521 ctacagctgg tgttccttca ccaggagtaa aagtctttgt gtatttctgt gttttagaaa
     5581 tttctggtac atatacaata gctaaatttt cttttttcat ttttttcaag tttatatttc
     5641 cacttactaa gtatttttct aatgacttta tcatctcatc attataacct tttagctttt
     5701 gctttagtgt atatttgcca gtagtcttat tttttatgaa cagaggattt agcttataat
     5761 aaggagttga atttatagct tcgtagtaat ctaatctatt tattttattt acctctattc
     5821 ttgtgtttaa gatactagca gttttgactt tgtcaattcc atctatattt tttattttat
     5881 ctatctgatt atcattaaac ccttttatat ctttaaaaga agtattgtta ccaatttcat
     5941 tgagataaac atttgataga ataaaatctg cattcatata tgtttgttct tgagtagatt
     6001 tgttttgcac atctaaaaaa gaatatttat aattgtcttt tatgacgatt atacctccta
     6061 tactcataga taatacaatt ataatgaacc cctttttatt tctaaagatg tttttcgcag
     6121 atatagattt tgttactgac atatttaaag ttaattttga tattaaataa ttaactttgg
     6181 ttacattttt atcagctcca aaagtttttc tgatagcatc aataggagaa attcttctta
     6241 tttttaagta tgtaaagaaa cttataatta gtattattag tattgtacat gcaattgata
     6301 agagaattat tttatctgga ataacaaaag gtgtaacatt tatatttcct tcaaattgaa
     6361 tatttcctac agacctatta aatatttgtg cacctcccat acctatacat atacctatag
     6421 gcattgctat tagagcaagt atgaaaagtt cataaagcat aagtttgaaa atcctaaagt
     6481 tggtggaacc aatagctctt aagacgccat attcctgtac tctttggtat attgatataa
     6541 catagatact atatattaca ataccagcaa atagactcat agcaattgca acgtatttac
     6601 ttgaattatc taaagtaccg tttttcatag tagattctat aagcattgag tttcttccta
     6661 tacttttctc atctaacata gcatttttta ttatattgtc tatgtttgta tttatatcac
     6721 tgccttcatt aaattcaata taagttgtaa aatcattcag ttctgattca ttgagattag
     6781 aaaaaatttc acattgtccc ttttttttct ctataaatct atcttcaagg attccaacaa
     6841 ctttaaaagt ttctggattt tctttttgat ataatttaaa agtaaggttt tgatttatct
     6901 ctggttttaa gcccatacta tttaaaatcc atttctcgac taccacctca ttgctagctt
     6961 ttggaaatcg accttttata agtttagaat gactagtcaa ataattttta tttgtatata
     7021 gtatgtttat aggtaatttt tcattaacat ctgtagacgc atagtaagat gttataccca
     7081 gatttttaat atctttgcta gattttatgg actttaattg atttttcttt atatttttaa
     7141 agtatacatg gtcagttcca agctctcttt tcaatctatc tacatctgct tgctgagcac
     7201 ttcttgagag agtaccaata ccaacaacga gtgatgtagc aagaattata ctcacaacaa
     7261 ttgctaaaga cctagcttta taacttttta tataccttag tactattttc aaattatctt
     7321 tcatatttac cacttccttt cttaactaaa tcaattatat atttatctaa aatatatagc
     7381 aaagtaagta ttttcaaatt ttaagtagtt aattgtacaa attcatctgt atttttacaa
     7441 gtttaattaa tatatatgta aattttaact ttttacataa tttttttaaa atgatataat
     7501 ccctatataa attgtagagg ggaggggaat catggataac aattttgtat ttcctatagt
     7561 aatactaact ttgtttatat ttagcaatct tataatgaat aagtataaat tttacaaaga
     7621 attagacata ttaaaaagag gcatattaac attttttata tcgtgttttg cattgatttt
     7681 atttatggag ggcactaaat atatacaatt aaggttagga tatagttttt tacttgaagg
     7741 agtattaaat attatacttt ttttggtttt aattatttta agtttttata caggctttaa
     7801 agttataaga ttgataaata ataataatga acaggaatat aaattacaaa aactagatag
     7861 taaaataaat cataaggata atataattat caaattaaga agtcaaaaac atgactattt
     7921 gaaacatttg caagtagtgt attctctatt aaatactggg ctagatgaag atgccaaaga
     7981 atatataatg aatatatcaa atagctttaa atcaaaaaat tcaaggtatg gaaaagtatc
     8041 ttatttagat gctatagctt cattaaaata cgaggaatgt cttgaaaagg atatatcttt
     8101 tgatgtctac attcaagatt ttatggataa tttagagatt gaacctaatg atatgagttc
     8161 agtactatta aacataatag ataatgcaat agagtctttg tctactataa aaaaagaaaa
     8221 gaagtatata agattacatg catatgaaga tgaatttcag tatattatag ctataaaaaa
     8281 taatggtcct aaaatacata atgttgaaag tatattttgg aatggattta gtactaaaga
     8341 aggacaagat aggggatatg ggctacattt agttaaaaat atattagata agtatgatta
     8401 tagtatactt gtaaatagtg atgatttttt aactgaattt gtaatcttaa tcccaaaata
     8461 ttattagtta ggaggagaaa agtctatgat aaaagttttg atttgtgatg atgataagat
     8521 tgtaagagat gatatagcaa atatagctaa agagtctgga tatgtagatt atgttaaaaa
     8581 agttaaggat ggtatagagg caattgaatt tataaaaaat gagaaaatag atttattgat
     8641 tattgacatt gatatgccat ataaaaatgg gattgattct gctgaagaaa ttctttctat
     8701 agataaggat atacatatag tatttgtgac gggtttcgct gattatagtc ttgaaagctt
     8761 taaagtgcat cctatagatt ttgtagttaa accatttaaa aaagaaaaaa tacttgattc
     8821 aattaacata gcaatagacc atattaactc ccataagatt gctaataata attttataga
     8881 agacaccctt tttgtatata aaataaggaa gcagatacat atgattaatt ttgatgatat
     8941 agttatgttt gagaaaaatt caagagcaat taatctacac actaaagatg aagatataat
     9001 aaagttttat gagaactttg atgatttaaa aaaacgccta cctcctaatt tctttatgac
     9061 ccatagacaa tacatagtaa atctaagatt tatacataag gttataccta taaataaatc
     9121 ttcattagat ataaggttta taaattttca agaaaatgcc atattaaaca aatctctaga
     9181 aaaagatttt ttatatagat tttatagggt taaaagattc tagtgaagat agaagaaaaa
     9241 tactttttgt gtgatataat aagtaccgta aagataatgt ataagagaag tttatataaa
     9301 aattatttgt aattatgatt taatctatat aaattacagt atagtatata ttggactttc
     9361 taattttttt aagaaagtcc aatatatttt agagatagtt tttatactta taaattactt
     9421 aagatactag ggtctttaat cttactatct tcagcaagta atagtatctt agacataatc
     9481 tcagcagttt tagggtcttc atcagcgaat ggaaggaata tcttacctct actttgagaa
     9541 tgtataggaa gtatattcaa agcacccatc ccttttttat gtacaatacc agaacccata
     9601 tgcacagaat actcacctaa attacctgct ataagagcat gagagccttc aaaattgaca
     9661 ttgttaattt ttaataataa taaaagttct ttagcaattg ctatacgcat ctctacagta
     9721 gaatggctag tttcagggtc aacaccacca gcatgagcga cacttacaac aaggtctata
     9781 tcacgcataa cttctgagaa tagaattgga ggtatagaag ctaattctaa acttttataa
     9841 gtttttctat caaaaaattc tactgtttca atagtaggag gctctatatc tgctggtgaa
     9901 aaccaatctg caagtgcaaa catctttgcc actatatttt ctttataaaa tactttctgt
     9961 aatccctctt cataatcgac agtccaaagc tgatttctaa gaagtgctac agttttcttt
    10021 ggctgaattt ggtgaccagc atatctgcga gatatagttt tttcagataa ttcatcactg
    10081 tttattctat aatactcacg gaatacttgc ttaaatggtt gaacaatttg cttactaaat
    10141 aaatcatgtt ggaactcact ccacttacca gattcaaaca aatgaactgg atgtgcaagt
    10201 tttaatttat tttttgccgt caacttataa gtttcacctt ctatattgca aataaagaac
    10261 ttagacttat cattataaac atatccaata ttatcatcac atacccatac tagattttta
    10321 atcaaagggc agataactgg atttttatag atattggcaa tttctttaac tgtaaatact
    10381 atttcatttt tcatagcttg ttctaaactc tgttttgcac gagaatactg actctttaaa
    10441 tctttttgga cagatttaag ttcaagtaca tatggatgtt tagcaatagc ttttggtata
    10501 ctctttaaag acttgttgcc tttttgaact ttagtagatg cttttccgtt ttcatcaaca
    10561 tctatccaaa ttgaagtacc atcaatatct tttggtttaa atagatgagc aacttcattt
    10621 agtttttcag tctcaagagc ccacatcata cgatttacat ctccaaatcc agcacttcta
    10681 gctagatttt ctagcgctat agtacaagct tttgattcac tttcacgacg ttgtgcacca
    10741 aattgcttgc tttctttagc aaacttttga ataaattcat aacggaaagt tgcttctttt
    10801 aggtctcctt ttttcatagg aagtaaagag aaacttctca atttatcttg atttctattt
    10861 gtttcaattt ctttcatcaa catgtcttta tcaagcttac caagtgtagc atcagcatag
    10921 agttgtgcac gtctatgaga gttaccacca ctagttatat atttagctga cttgtaaagt
    10981 atattgaatc tttcttcccc aatagtttta tatgaatcta aaaaccaatc tacatcaaat
    11041 gcaccatcaa tgaaactacg tggtgaaata ggggagaata gagcaacctc tgattctttt
    11101 tgtgcagaga agccttcatt tatatgtgca tggaagtacc aaattgtact tttcagaccc
    11161 ttccagttta aatatttttc aattatgttt acccattgag gggcatacat agcagattct
    11221 atcaatctta actcagtaat gtcggtattt ttaagtaaac tttttaattt ttttgaatca
    11281 tcttctttag taggatagca atttttaagt agagaactta aaacatcttt ttttgaataa
    11341 ttgctaccat atgaatatcc tctctggaaa gtttctttac caagagcgac caaaatattt
    11401 acaaaataat ttataccttc aaagtattgt atattattag ctagattaga cacttctgtt
    11461 ggcatatcac ctctacgtga ttcaatttct ataattcggt ttgtaacagt ttttaaatta
    11521 tctttaagcc aagggtactt ttctaatgtg tcgccagccc atctaggatt tgtcaatgtt
    11581 cttattatat ttgatgcgtt gtatccactt atcatttctc tattaacttg ctcttcagtc
    11641 attaatccta attcatgtgc acgagcaaac acatttatgc tagtatagaa aggaaactta
    11701 tattctagca actgataatt tgaaaacgta gtgtagaaaa acttagtaaa agaaatatca
    11761 tcataaataa acgaatgtgc tttatcaagc caatagttta aaataggggc atttaaagca
    11821 gggcagtatt cattttgtct gtaatagttt ttagtaaatt caactctttg ttttttacat
    11881 tgttctatag tcaataagtt tctaagttta tagtatattt ctaaacaata gttaaatatt
    11941 tctttatggt caaactcatt tagtaaaact cttaaaagtt ttcttgtaag tccattaaat
    12001 ttaagttttt caagttttgt atatatttta ttttgttttt tagagtcaag gacatttgaa
    12061 tatatagatg aaaaccaact tttttctgtt tcattatatc cacatgtact tatactataa
    12121 aataagatat caagtattaa tttgtaatct aagtcaagtt ttttactagt ttctctccaa
    12181 agctctgcta atggataatt atcaagagta tcattagaaa ctttatattt aataggagtc
    12241 aagttataag tagaataacc taaaagtact ttgtctacat tacctgacca tctttcaact
    12301 tcatattcat aatctgcatt ttcagtaata agttcatcaa atttttcata aacttttatt
    12361 attttattta tatccgtatc gagaattttt tttgcttctt tttttgttgc tacagaaaca
    12421 gaatcctcac gagtagataa acttatattt gagataattt tattaagctt tgatgagcta
    12481 ttagaatcat ctttaaaatc aaattgttct ggaatttgaa ctttgttttc agggttatat
    12541 attccaaaac cattttcttt gcagtaatca ttttttgctt tacctaacaa attatcaatt
    12601 agtatttgaa cttgagatgt attcttttta gatttatcaa tatcctctat aagtaaaata
    12661 agcttattat atagttcttg tctagcagta ttgtctttta ataagtttac aatttcaaga
    12721 cctccaaggc atttttgaga atttgaagat tttatcagtg aagaagcaat attttccaaa
    12781 tttgaatcat cttgttcaag taaaattttt attactgttt gtctaataga gcttgtcttt
    12841 aaagacaata gattggttat cttatctaat tctacatctg ttagctcaag ctttctaagt
    12901 agactcaata ccgtttctct attggaaaca ctcttatcac ttaaacattc caataaaaat
    12961 tcacgttgtc tatctgtctt tggttcattt acaaaattga ctataagtga agctcttaaa
    13021 tctgaactca taagatgaca attttccata agcacatcta taaattgagc attcatatcg
    13081 tatgctgtta aagtcatcat acatttaagt atttcatgag aagaaatttc aatccaagtc
    13141 catggaaata cacttcctga aaatgatttt gtctttgaat ttaaaatttc taaagtatgt
    13201 tttaaacttt caaattgttc ttttctagca tcaaagtctt tgtcaatagg agtgtttgac
    13261 aagtcaaaat tagtttctct gttttgccaa ctatagtagt agccaacaga accttctaca
    13321 tagaaatttg taattaccca tgctagtatc tctaaatcat cacccttaat gtttttagat
    13381 gctatagaat gtttaaaata tgtactttct gtatgcgata aaaagtaaag agctacaagt
    13441 tttttatatt tttcctgaca agaaagtaag ttttcaataa gagagtaaga gtcatatatg
    13501 ttgtaagttg cagtagccca taaggcaaaa taaatttcaa gattatcatt gctattaatt
    13561 gattctatac ggaacttttc atcagtcaat gatttatatg aaagttctaa acatttacgt
    13621 attattccag gtttttggtc tgaaaaacca agtcctgtcc atgtatccaa ggctcttata
    13681 acagaactaa atcgctctaa attgttgtcc aaaatagttt ttaacatgta tattgatgca
    13741 gaaattgtac cctcatctaa actctctaca atttgctgtc taagtccttc ttgttgtttg
    13801 gctgccaaaa gaagtttaga aagcatttca tagcatttat cactatgact cttcatgata
    13861 ccaattatca tttgtcttgt aagtagagca gtttgatttt ctccaaatat tacttcttct
    13921 aaagcactta ttactttttg atttcctttg tctatttcta gtgctatttt atcactcatt
    13981 atagagtcat agtacaagac cttatttgga agagtaagat aatctataac tgaaaaatca
    14041 taataaatta aatcaacata gtttgaaata aaccttataa tacctctaaa ataatccaca
    14101 agtttatttg agcgataccc tttacgataa tatcctcctg aatatgtata atccatcttt
    14161 cttttaatta attggcaaag ttcttctact tgttcagaag taaatattaa ttctaatgca
    14221 ttttttaaag gtccagaaag gagctcgtcc atatttttaa tttcgtagta gtggcaattg
    14281 tagtatttct ttatcatatt taagaatttg tcttcaggtt ctctataata gtatgaacta
    14341 ttttctatgc agtcccaaag agactttgct aagtcgtagt actgttttga agaagattta
    14401 gtaaaattat ttttaaaatt ttcaattatt ttaggatttt tttctactct agtacgttct
    14461 aacatttatg tacctccaaa ttatttattt taaaacattc ttccagcaag caaggtaagt
    14521 cttataaaag taaatatatc tccacttttc agatttatag aggaatcgag tttttcaata
    14581 acatcctcat ttataagcac tttgtagatt ccatcttcaa atgcctgtaa cgctacctct
    14641 attgcctttt ctacagattg tttattctca ttgtaaatag agccaaatcc aacttttcca
    14701 atatcagctt gagattcaat ttcttcatta gttaagaagt ttattattga tttttctggg
    14761 attttctcat tatattcaag aacattttta gatacaattt cagagatagc ttcttttagg
    14821 caattgatgt tgtcagatac atcaaaaggg gtaagttcta gcataggttt gcgtttgcca
    14881 acagccttaa ctctaaaata aatttgcata aaatcacctc gcaaaatatt aattatgtat
    14941 ttagaaattt aaaaatttat ctatttgtta tattatatca ttttgtgaat tattaatgtg
    15001 aattatggaa ttattttaca aaaaatagaa aaaaagctat cttaaagtct aaaaaagcaa
    15061 taaaagataa ctt
//
LOCUS       DAYAOX010000092        14903 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.092, whole genome shotgun sequence.
ACCESSION   DAYAOX010000092 DAYAOX010000000
VERSION     DAYAOX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.092"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(165..1535)
                     /locus_tag="ACGI57_002120"
     CDS             complement(165..1535)
                     /locus_tag="ACGI57_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HFG9717197.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            1751..2566
                     /locus_tag="ACGI57_002121"
     CDS             1751..2566
                     /locus_tag="ACGI57_002121"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFG9717198.1"
                     /translation="MNIKSAFIRKRGEKFYVYVEYIEETTGKIKQKSYGSYEKKKDAE
                     KHLIEIKSTINNNKFVAPKDMTFVERCYKYMDMNKNNWSPYTVTNTKSWVKNYIEPLF
                     KNLKLTEVNPSSVQAFVNYNFNNSTFTSAKVRYSFLSSVIREAYRLREIQENSCDFIK
                     LPSKEKYSTNEIYNRKEVLLLIEKLLSNNIEIPILLMLLLGLRMGEATGLRWSDVDLD
                     NNLINVNQILVYSNGKISFKEPKTPKSKRALSAPKELIEKLKIKKLRQSKLKL"
     gene            2596..2868
                     /locus_tag="ACGI57_002122"
     CDS             2596..2868
                     /locus_tag="ACGI57_002122"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9717199.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(3231..3311)
                     /locus_tag="ACGI57_002123"
                     /pseudo
     CDS             complement(3231..3311)
                     /locus_tag="ACGI57_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(3351..4019)
                     /locus_tag="ACGI57_002124"
     CDS             complement(3351..4019)
                     /locus_tag="ACGI57_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFG9717200.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(4872..7292)
                     /gene="leuS"
                     /locus_tag="ACGI57_002125"
     CDS             complement(4872..7292)
                     /gene="leuS"
                     /locus_tag="ACGI57_002125"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFG9717201.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(7646..7996)
                     /gene="rsfS"
                     /locus_tag="ACGI57_002126"
     CDS             complement(7646..7996)
                     /gene="rsfS"
                     /locus_tag="ACGI57_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFG9717202.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(8113..8685)
                     /gene="yqeK"
                     /locus_tag="ACGI57_002127"
     CDS             complement(8113..8685)
                     /gene="yqeK"
                     /locus_tag="ACGI57_002127"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFG9717203.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(8686..9375)
                     /gene="nadD"
                     /locus_tag="ACGI57_002128"
     CDS             complement(8686..9375)
                     /gene="nadD"
                     /locus_tag="ACGI57_002128"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFG9717204.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            9651..10289
                     /gene="recX"
                     /locus_tag="ACGI57_002129"
     CDS             9651..10289
                     /gene="recX"
                     /locus_tag="ACGI57_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFG9717205.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            10442..12445
                     /locus_tag="ACGI57_002130"
     CDS             10442..12445
                     /locus_tag="ACGI57_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFG9717206.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            12610..13260
                     /locus_tag="ACGI57_002131"
     CDS             12610..13260
                     /locus_tag="ACGI57_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9717207.1"
                     /translation="MSFLGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDG
                     LSRGAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHTYEQHN
                     KQDIFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMF
                     GFLLNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            13353..14036
                     /locus_tag="ACGI57_002132"
     CDS             13353..14036
                     /locus_tag="ACGI57_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717208.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(14213..14524)
                     /locus_tag="ACGI57_002133"
     CDS             complement(14213..14524)
                     /locus_tag="ACGI57_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFG9717209.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(14602..>14903)
                     /locus_tag="ACGI57_002134"
     CDS             complement(14602..>14903)
                     /locus_tag="ACGI57_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9717210.1"
                     /translation="NIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQL
                     IYNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
BASE COUNT         5166 a         2365 c         1930 g         5442 t
ORIGIN      
        1 tattactttt tacattataa taatattctt ttaatcaaaa aaacacctac tattaaagta
       61 aatgttttta agtattttta attttaaaat ccatatagtt catatgaaac taaatataat
      121 aactcaacat atgagcaata tcgagacatt tacataccca ctagttattt atattcaaaa
      181 aaatcttctc catccgggtt ataaaataat attctacttg ccaatccttg ttcatattta
      241 taattttctt tatcacctac ataagaaaat gttaatgtca tgcttcttga tggatctaat
      301 gaaattatat ctaaataaat agtacatttt tctcctggct ttaatacacc gaaactatta
      361 aaatccattt ttattttagc gtattgcccc atatataatg ttgaattagc ttctccataa
      421 acttttaatt ttgttacatc taaattttgt gtacttgtat tttctatatt tataggaact
      481 gttgggtctt tatatttttc agtataatat tcaaggttat catcataaca ttcaagataa
      541 cttcctttat cttgccaaat tatgtgattt gaatcaaaat caatctttaa tccacatatt
      601 atatttttat ttgaagatgg aagactaata ttattgatgg tattttcaca ctctagcata
      661 attttatcac ttagatttcc tatcccggtt acttttgcag catttttaag tatatcttta
      721 ttacttccaa tatcaactag tacaatagga ttatttttag ccattggtga agcaactaaa
      781 gcataaacta aatcagtacc actagctata taaaattctt ttgcgccatt atagaactta
      841 tttactattt ttttattagt atcatatcta tctactccac ccaatcgagt tgaattagta
      901 tcatttacta aactatcact cattgatgaa gtgccaccta ttgcataact ttctatacca
      961 gttgtattaa atggaacact ttttccatca gttaatacta tagctgcttt atctctaact
     1021 gctacaggtg cagcactcat agcatctggc tcacctttga aagcgttagt taatattact
     1081 ttatttactt tattaatgga atttatttct tttgctacat tataacttgt cttaatccta
     1141 tcattacctt gaagccttct agtctctatt cctttatctt ttagaaaagt ttctgttgct
     1201 ttatcaatgg aactttctcc tcctattatg tatacctttt tagctttttc tactctttta
     1261 agagttgcat taggtatatt attcttttta gtcaaaagga ttggtgcatt tgttgctcct
     1321 gcaagtccac tcgcacttag cccatcagcc attgtactat ctgcatttat aagtattgca
     1381 gtagtataac tttgtttgtc agctatgata ccagcagttt cgtatttatc tgctccttga
     1441 attttatcaa ttttatcaag tgcattagct gatataggaa aaccagcaac aaacattgac
     1501 actaacccta gtgataataa tttctttaat ttcacaatat ctcccccttt tatatatgaa
     1561 atactatata tcttaataat tctacattaa attttctttt cctcctttaa cgatatttat
     1621 cttcctgtta atttaagtgt atacaaatac gtgttataat gtaactgtaa agaattaaaa
     1681 agataatgac ataattagag tagtacaact gttcttttat aaaactaact tataaaggga
     1741 gcgtacatat atgaatatca aatcagcttt tataagaaaa agaggggaaa aattctatgt
     1801 atatgtggag tacatagagg aaacaactgg caagataaaa cagaaaagtt atggaagtta
     1861 tgaaaagaaa aaggatgctg aaaaacattt aattgaaata aaatctacta taaacaataa
     1921 taagtttgtt gctccaaaag atatgacatt tgtagaaaga tgttataaat acatggacat
     1981 gaataaaaat aattggtctc catatactgt tacaaataca aaatcttggg ttaaaaatta
     2041 catagaacct ctttttaaga atctaaaact cacagaagta aatcctagtt cagtacaagc
     2101 ttttgtaaat tataacttta ataattctac ctttactagt gctaaagtaa ggtatagctt
     2161 tctaagttct gtaataaggg aagcatatag attaagagaa atacaagaaa attcatgtga
     2221 ttttataaaa cttccttcta aagaaaaata ttctacaaat gaaatataca atagaaaaga
     2281 agtattattg ctaatagaaa agttacttag taataatata gaaataccta ttcttttaat
     2341 gttacttcta ggtttaagaa tgggagaagc tactgggctt aggtggtcag atgttgactt
     2401 agataacaat ctgataaatg ttaatcaaat tcttgtctat tctaatggca aaatatcttt
     2461 taaagagcca aaaacaccaa aatcaaaaag agctttatca gctccaaaag aattaattga
     2521 aaaattgaaa attaaaaaac taagacaaag caaattaaaa ttgtaaggta tcctggaaaa
     2581 cagaaataat ttagtttgtc taaacaccaa tttaaatccg tttctacctt cttcactaag
     2641 aaaaacgttt cacaatttta caaaaaaaat aatttaaaaa agattagaat acatgattta
     2701 agatatacaa gtgccactct gctgctttta gggggaacta atatgaaagt tgtttctgaa
     2761 agattgggtc atacagatat aaaaataact atgaatagat attctcatgt acttaaagaa
     2821 atggacaaag aagcttccga caatctaagc gaacttctat ttaaatagta aaaactgact
     2881 gtcagttaaa tgtcagttaa acctaaaaaa ataggttttg tcagttgatt atcagtcaat
     2941 tattttataa tagtttcact taaaaacgtc tatatctaca cattttacta tttgtttatg
     3001 cttattaata tctatctgtt attattaatt ctaatagctt ctatttaatt atttagttca
     3061 gtttacagga aactacacac aaaccataaa ataggatgtt gacaatataa cagatagtat
     3121 gctcctgtaa aaggtaacgt actatctgtt atgttgtctg gatttccaaa tggttaccct
     3181 ttggcacaca actttgctat ctccttggta aactaaaagt ttgtaatcta aaatcctaat
     3241 ccatctctat aaaatttaat tgacttctcc atacttttta cacctaaaca tataaaagta
     3301 attttattca cttaatgacc tccataattc ccaaattagc tatttggaac ttaactaaaa
     3361 aatcctaata taagggcaac tattataatg gcaatcggaa tacatactgt gtaatcctgt
     3421 tccatagagt catcatctga atgtagtttt acaattcgca atttagcaat cagtttgaag
     3481 cagaaatagc ctattattgt tccaattaca ttcgtgagta agtcgtctat atcggttgct
     3541 ctgtgtaacg taaaaagctg aatagtttcg ataaaaagag ataatccaaa accaatcaag
     3601 aatgtatttc tcaaacgttc aaaggttttg ctgataagcg gacataaaaa acctaatggg
     3661 acaaaaagta aaatattcaa aataaaactc aaactaaatc catcactaaa aggaataaga
     3721 tttatgtttg gattaaagaa cgtttcccct aactgtgaca gtcttatata ttcactgaat
     3781 gtagggatac caacaatatg cgatagtgtt aaacataagt agtaatacat tgccagtgat
     3841 aaaatagatg tttttacctt tggaatatca ctatttttct tgaatattac aactaagaaa
     3901 ataccaatca agataattcc caacaagaaa ataggattaa ttaacaataa atctttgata
     3961 gcttcaaaca gactgagttg aatttctaca cctgattgat tatttgttac agtctccatt
     4021 tgtacatacc tcctcataac atttcaatta tttctaaaac acaaaataga agataaatct
     4081 taaaatcatc ctatcaaaat ttttaaattt gcctttatca gtttatacca ccagttaatt
     4141 cctgtcttac ttctgtatgg tattcttggg attctttcat caaattagac aaaaaatggt
     4201 taccatatca ataaactggt tacgctccat atctcccgaa gcaaccatcc cctgagaata
     4261 aatccgtatc aggaatctcc acatctactg cacctatcac aagttatttg ataacatcct
     4321 cagacaatcc caacttggat atatcgacaa cacccattag aataccactg ctgaccatac
     4381 ttgttttcct gctgataatt ttaatcaact gggaagttga aacgctatct tttttacaag
     4441 ttccttttta gaaacatggt agctattata tgaatctgaa tttcgttgtc tgggtgtttc
     4501 atataaagca tagatacgca ccttttttgc ctgttttcat tatatagaaa aaaatttcaa
     4561 aatacaataa ttatattttt ttcgtttaag cttagagcag ttccttgatg aacaacactt
     4621 tggaaactga atgatttaca taccacttaa tacattaact cgatttaaca ttaattataa
     4681 taatatttaa aacaacaact agggtatata agaatgaact ttttagttct ttaatcattt
     4741 ttttgtataa aatcaaatct atttttataa tttcaacaat tttataattt caacaatgaa
     4801 aaaaggctta tctctatttt gagacagcct cgtttatttt ataaatttat atttttgttc
     4861 atatttttga gttatttaac aactatattt actagttttt taggtacagc tacaacttta
     4921 actatagttt taccttcaac taatcctttt attttttcat cttctaaagc taccttttcc
     4981 atctcatctt tagatatatt tgagttaaca gttaattttc ctctaacttt tccattaact
     5041 tgtacaacaa cttctatttc atcttttact aaagcttttt cgtcatattt aggccagtct
     5101 atgtcaaata cacttccttc tttacctatc atagtccata gttcttcacc taaatgaggg
     5161 gcaaatggtg caattatagt tactatagtt tgtacacctt cttttataac tgcttcgttt
     5221 atattatcta actctttata tttatacata tcatttatta attccataag agctgatata
     5281 gcagtattaa atccgaattt ttcacttaaa tcagcagtaa cttttttaag tgtagagtgt
     5341 atagtatatc tcatatcctt atcttgggaa tttaattctc caaactctac atccttttta
     5401 accacatctg ctaactcgtc aaccaatcta taaaccctat ttaagaatct aaagcatcca
     5461 tcaacacctt gctctgacca gtccaaatct ctttctggtg gtgctgcaaa taatacgaaa
     5521 agtcttgcag tatctgctcc atactcatct attatatcca taggagatac tgtattccct
     5581 ttagacttac tcattttaga accatccata agtaccattc cttgagtaag taagttttta
     5641 aatggctcat tgaagtctac cattcccata ctcttaaatg cctttacaaa ccatcttgca
     5701 taaagtaaat gcattatagc atgttctact ccacctatat attggtctac tggatgccat
     5761 ctattaacta attccttact aaatggttca ttttcattat tgctatccac atatcttaag
     5821 aaataccaag aagaatcaac gaatgtatcc atagtatcaa cttcccttct agctggtttt
     5881 ccacagtgag gacaagttgt tgacataaat tgctttgaag tcgtaagtgg agattcccct
     5941 tttccagtaa attcaacatc tgttggtaaa agaactggta aatcttcctt tttaactgga
     6001 actattccac attcatcaca ataaaccaca ggtataggac aaccccagta tctttgtctt
     6061 gaaactaacc agtctctaag tctataatta attgttctct ttcctctacc ttctttttct
     6121 aacttatcga ttattccttc aaatccttct gaagcatcta atccattaaa ttcttctgaa
     6181 tttatcattt tgttatcttc tgttattaca tcaattatgt ctaaattata tttttcagca
     6241 aaatctctat ctctttcatc atgtgctgga actgccatta tagcacctgt tccatactca
     6301 actaatacat agttagctat ccatagagga actttcttgc catttaaagg atttataaca
     6361 tatctaccta taaacattcc ttctttttct acatcagctg ctgttctttc tatttctgtc
     6421 atagtatgca ttttttgaac aaaagcttca acatctgctt catattcagt ccctgcaacc
     6481 aattctttaa ctaattcatg ctctggagca agtaccatat aagtaactcc ataagttgtg
     6541 tctggtctag ttgtaaatac tgtcatagat ttatcagttc catctatatc aaaaactaaa
     6601 tctgctcctg tacttttacc tatccaattt ttttgcattg ttttaacttt ttctggccat
     6661 ccatctaata tatctaagtc attaagtaat tcttctgcaa attcagtagt cttaaaatac
     6721 cattgttcta aatctttctt taatactgtt gctttacatc tctcacatgc tccttgaaca
     6781 acttgctcat ttgcaagaac agtctcacaa gaaggacacc agttaacata tgattttttc
     6841 ttatatgcaa gcccatgctc taaaaacttc aaaaatattt cttgtgtaaa tctatagtat
     6901 tctggcgttg aagttgcaac ttctttatcc caatcataac taatacctaa taaatttaat
     6961 tgctctttca tttcttctat attttccatt gtccatttat gtggatgtat tccatgtttt
     7021 atagctgcat tttcagctgg taatccaaaa gaatcccatc ccattggatg aagtacatta
     7081 tatccttcca tttttttgaa tcttgcgaca acatcaccta tagaataatt tctaacatgt
     7141 cccatgtgta tttttccaga tggataaggg aacatttcta atgtgtaata attaggtttt
     7201 tcagtttgtg cagtatccat tttatactga ttattatctt tccagatttt ttgccatttc
     7261 gattctactt ctttaaaatt atatacgctc atattttgcc tcctattttt aattgtgtat
     7321 aactacctta tttagtatta tttacttata atttccaaat aattctttga aaataaaaaa
     7381 tcctcgtcac taaacttacg tttagggacg aggataaatc ttcgcggtac caccctaatt
     7441 agcctactac atatatagac tctcttagat agcttttaat ccagccatgg agaatagatt
     7501 ttaatctagt aagttcaaag ttatataata ttagttctca ccttccacta actctctgaa
     7561 aaaatataat cttttactgc tctagtctat tcaatttaat ataatttttg aaattttcta
     7621 attataataa catatattat catgtttatg ctaatgtgtc aatatctata taaggtgcat
     7681 ctttccataa tttttctaaa ttataaaacc ctctattttc ctcattaaat acatgaacca
     7741 ttatgtctcc ataatcaagg agtacccaag tttgagttcc ttgtccttct ttacctcttg
     7801 gctctaatcc tagttttgct aattcatctt ccacattgtc agcaatagct tttacctgtc
     7861 tctgagaaga tgctgtagta ataataaaat aatcacacaa actagaaact tttcctatat
     7921 ttattataac tgtatcttga cccagtttat cttctatggc atcatatgct atttttgtca
     7981 tttgttccac tgtcatataa aaattcctca ctttcctaat taaaatacac ttagttattg
     8041 taacatatta atacgatttt gtaacatagt caaatttaaa tttattaata tgtttgcctt
     8101 tatatagctt ttttataata acttttcttt aattagatag tttcttgcca taactgttct
     8161 tggatgtatc tcttcttttt tattgatgac aaacattaaa gtattattaa aagaagttaa
     8221 aagtgcttca tcaagcttcc ctccatatgc taattctctt aatgtatcta caactgggaa
     8281 ttttcttcct tcttcaatta aatcagctat gtaaattatt ttttctaaaa gagacatgtc
     8341 ctctctccct gttgtatgat atttgattgc atttataata tcttcatctt ccacttcaaa
     8401 ttcatactcg catatgtacg ccccaattac actatgagac aaggaacgat ttccatcctc
     8461 tattgggtct aaatatattt catacttatt tacataatcc tctatttcct tgtcacttaa
     8521 atactttgca caatcatgaa tcattcctgc taagtaagct ttttctttgt cacatccata
     8581 aatctcacat aatttttcag cacattttgc aacatttttt gaatggctaa gcctccccac
     8641 tggtagcatt tgatttagtc tttggttaat attttctaag ttcatttaat catctccata
     8701 ttgatatagt ttattttcat ataaataatt ttctacattt tcgggtacta agtaacgtat
     8761 tgacttgcct tcatttagct gttcccttat atatgtggac gatatatcca gtgatggaac
     8821 ataaactgtt ataatgtcag cattatactt cttagttagt ttttctatag tctcttgtga
     8881 ccttaacaaa ctaattcctg gtctagttgc ggctataaaa gtagctaact caaagttttt
     8941 ctttacatct ttccactctt caacactaca aattgcatct gcacctgtta taaaataaat
     9001 gtctgcattt ttgtattttt tctttaagta ttttaatgta tctattgtat aagtcctttt
     9061 ctttctttcg atttctactt ttgaaactaa aaaatcttcg ttgctttcag ttgctagaag
     9121 tgtcatatta tatctatcat acttatctgt agtgatacat aatttatgtg gtggatttcc
     9181 tgatggtata aagattattt tgtcaatgtc atacttgtct ctaataaatt ctgctgttgc
     9241 taaatgagca tagtgtatag ggtcaaaagt acctcccaat atacctattt tgattttgcc
     9301 tttatggcga ttaaatttct ctagtttttc agtatttttc aactctgcca tcttaactag
     9361 attttcactt ctcatatttt gtctcctttt aatgttataa tactattgta ccattatttt
     9421 ttatttctta aagcttataa tttgtttttt tgggtatata attatattta tatgctaaaa
     9481 aacactttaa aaaataatta tttaacataa ttctgcttta ctttttaata aaaataactt
     9541 tttactattt tgtcttaatt ttgataaaat ataacataca aatataagtt ttttgttaaa
     9601 ttttattagt tgcttttgtt aatataatta catataaaaa aggaggactt atgggtataa
     9661 ttacaaaaat agaacaacaa aaaagaaatg atgatagagt taatatttat gtagatgata
     9721 aattttttat tgctatattt aaagaactgg tttatacatt taatttaaaa aaagggaatg
     9781 aaataaatga agaagaatta aaacctattt tagatgatga aatgtatata aaagctaaaa
     9841 ataaagcatt aaatatttta tctcatgctg accaatctga aaaaaaatta aaagaaaaac
     9901 tatcatcaga atttgatgaa aatataattg agagggtcat agattttctt aaaagttata
     9961 atctcgtaaa tgacagtgta ttagctcaaa aaattgttaa cacaaatgtt aacctaaata
    10021 aatgtggcaa aaacagaatt aagcagaatt tatataataa aggtatcaat agaagtacaa
    10081 tcaatgaagc agtatctgaa ttagacaagg acactgaatt tgaaaatgct atgtatctag
    10141 caaaaaaaag gtatgaaaga gtaaagaaag aagataaaaa gaaaatatat caaaaaattt
    10201 ctcaacatct ttcctataaa ggctttgatt atgatattat taaaagagtt ttaaacaaac
    10261 ttttaaattt tgatgaatac gatatataag tataaatact agggaggtaa attatgaaat
    10321 taatcattgg tggcttaagc tttttattag gattatataa tatatatcaa ttatacagag
    10381 aagatagtat gagataaata ttatttttta gatgtaatat ttattatgga ggagaaatac
    10441 aatgaaaagt caaaaaagat ttaaaatttt agatattgat gtatacctac aaccatttga
    10501 atctgatata aaaaagcgaa tgaaacatta tgaggatatg aaatcaaaaa cattgaaaaa
    10561 ctataaagat tttagttctt ttgcaaatgc tcatctttac tatggatttc atcagacaaa
    10621 tgatggattc ttctataggg aatgggctcc aaatgcagat agtttgtcac taattggtga
    10681 ctttaataat tggaatagaa aatctcatcc tctaagaaaa attgggtctg gtaattggga
    10741 aatatttatt cctggtcaaa atagcttacc tcacaaatca gaggtaaagg tacaagttac
    10801 tgcaaatgga aaaacatttg accgtatacc attatatata aaaagagtaa ttcaaaaaga
    10861 ttttggtgga tttaatggac aaatatggca accaaaaact ccatttatct ggactgacaa
    10921 tgattttgac ttaaaaaaca taacttctcc actaatctat gaatgtcata taggtatgtc
    10981 tactgaaagc gaatctattg gaacttataa tgaatttact gaaaaaatac ttcctaagat
    11041 aaaaaaagct ggatacaata ccattcagct tatggctatt atggaacatc catattatgc
    11101 atcttttgga taccaagttt caaactttta tgcaatctca tctaggtttg gtactcctga
    11161 agacttaaaa aacctcataa acactgctca ttcaatgggg attgctgttt tattagattt
    11221 agttcattct catgctgtaa agaatacttt agaaggaata aatgagtttg atggaagtga
    11281 acatcaattt ttccactcag gttcaaaggg aaatcatcct gcttggggta caaaactttt
    11341 taactatggt aaacctgaag taatacattt tttgctttca aatataaaat tttggttaaa
    11401 tgagtaccat tttgatggct ttaggtttga tggtgtaact tcaatgttat atcataacca
    11461 tggtcttgga gtttcatttg atagttatga aaaatatttt agtatgaata cagatattga
    11521 agctataaca tatctccaat ttgcaaatga attaatgaaa gaaattaaac ctaattcgat
    11581 aagtattgca gaagatatga gtggaatgcc tggtatgtgt atccctataa aagacggcgg
    11641 aattggattt gactatagac tcgcaatggg agtacctgat ttttggataa aaactatctc
    11701 taacttatct gatgaagact gggatttggg caaaatgtgg tatgaactta ctactagacg
    11761 acctggagaa aaaaatatag gatattgtga atcccatgac caagcattag ttggcgataa
    11821 aacaattatt ttttggctag ctgacaagga aatgtactgg aatatggaaa taaactcaaa
    11881 taaccatgtt atcaatagag caatatctct tattaaatta attaaattaa taactttttc
    11941 tctagcaggt gaaggatatc taaattttat gggaaatgaa tttggacatc ctgagtggat
    12001 tgattttcca cgagaaggca acaattggag ctataagtat gccagaagac aatggagcct
    12061 ttcagaagat aacaacttaa agtataaaca attattaaat tttgataaat ctatgctcga
    12121 acttacaaaa tacagtgata tttttatcca aaatagtcat gaacttatac atatggataa
    12181 tgataaaaag attttggtgt acagtaaggg caaatattta tttatattta atttccaccc
    12241 taatctaata caatcagttg atatttcaaa gtttcaaaat acaaactaca aaacgatatt
    12301 aaatactgat atggtagagt ttggaggaaa cacaaaaaaa gaaaacttaa gagattatga
    12361 tgtatacttt agctcaactc ctccttcttc taaaatgcct attgcctctc gtacagcaat
    12421 agtgctttta gataatggta tttaaatatg agccaaatat attaggaaac tgcaaatata
    12481 gtccagtata aggcaaaaaa ataaaataat gaaagcatgg caatatacct gttcaacatg
    12541 ctaaaaagac acattgacaa atcattatgt atttgctaag ataaacatac caaatgattg
    12601 tttcgaggag tgagtttttt gggaagaaaa aaagaacctg ataatactat acaaaaaata
    12661 cttgatattt cacataagtt attcttagaa aaagggtatg agcagacaac gattcaagac
    12721 attgtagata atttagatgg cctttcaaga ggtgctattt atcatcactt taaatcaaaa
    12781 gacgaaataa ttaaggcaat cacaagtcgg ttgtataatg atggatttta tgggtatgag
    12841 gtaaaaaatg atgaaggaaa gaccgcatta gaaaaattac aatataggtt gcttcatact
    12901 tatgaacagc acaataaaca agacattttt ttaatgtcat catcactatt gcgtaatcct
    12961 aagtatttag ctacgcagtt tgatgagatt ataaatgata ttgtgccttg gataaaagca
    13021 ttgattgaag aaggaaatca agataattct atggaagtta aaaatccaga gattgctgct
    13081 caaatgtttg gttttcttct aaacacatgg tgtattccaa cagtttatcc aggtaatcca
    13141 caacagatga tagaacgttt tactttgttt ggtgagcaag ctgcttttat gggtatttct
    13201 tttatgaatg ataagataat gtgtgaatat caaaaatttg ttaataaatt ttctaaatag
    13261 taaatataaa actgccttaa tatcggcagt tttatattta cacaaaacaa acatttagtt
    13321 ggtattttaa aaaatttaat ttatttcagt acatgcaaaa ggaggttgct atgaacaaac
    13381 taattaaaga ttgtgtaaac ttttttaatg attgtggctt ttcatattca atatgtggtg
    13441 gatatgccct agaattattt gcgaatatgc atattcgccc acacagtgat attgacatat
    13501 ggatttctga cacagataaa gaaaagagca ttcagtatat gcttaatcaa ggatgggata
    13561 tatatgagcc cttgggtaat tatttattac gacctattat cgaccttaac aagcaacata
    13621 tagagcaaat ttgtgttttt gcgattaagc ctgattgctc atttattgaa ttaaaaaaag
    13681 tcgaggatga taactataaa atgaatatac agtgtgacga acagttacag ttcgatttta
    13741 ttgaattgat atataatcca cagaaagatg gaaaatttct tttttcccaa aatcaaagaa
    13801 ttataagaga tatggataag gccagactga taacatcaga taatatccca tatatggctc
    13861 ccgaagtggt tttgttttgc aaatcaatat acatagatag gcttggttat caaaaagact
    13921 tcgatgtaac agtcccacta ttatcacaag agcaaaggga ttggcttgta aatgctttaa
    13981 taacagcata tccagatgga catgcatgga ttgaccaatt ggcaaatcag tattaagcct
    14041 tttagctccc gaatatgaca ttatgataat ccacactcta acagtttgtt ggtttttcta
    14101 gtatataaca ctcatattaa aataaacact aaaagccaaa taacaattta aatacaaaat
    14161 gttatttggc taatttagtt tgatactatt taactatctt ttacattcag ttctataatt
    14221 tccaactttt aatttgtggt attatattca tcttaacagt ttgattcttt aaagcttttc
    14281 taaatttctc agttgaagaa tctttaaaca ttttactaga aacactcttg aggtcttcta
    14341 tagattccca atgttgtatc ataatccact tttgagactt ttcatcatat aaaagctcag
    14401 tatcaataaa accagattga atagaatgaa attttaattc taagtcattt acaatttcta
    14461 taaactcttc ttttgataaa ttttcttcca cattaaactc aactatttct gtaataattt
    14521 tcatatgtaa aacctccaat ttattttcct atatcatatc aaaaaataaa ttggaggtat
    14581 tgtaaaaaat cgacagtata attataaata gctgtacttt atattttctt taatcgtctt
    14641 attctcacca ctaaatttaa ttggtgacgt ccctgaaaat tttttaaatt cttttataaa
    14701 gtgtgcttgg tcatagtact cattttcata aataaaatca aaaagttcat catctttata
    14761 attataaata agttgattgc ttgccatttt aaatctgtaa attctcttta aaatcttagg
    14821 tgtataccct gtatatttta aacagattct ttccaatgtt ttaatattta tatttgtatc
    14881 atcacaaaat gcttttatat tgc
//
LOCUS       DAYAOX010000093        14683 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.093, whole genome shotgun sequence.
ACCESSION   DAYAOX010000093 DAYAOX010000000
VERSION     DAYAOX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14683
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.093"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            158..1072
                     /gene="murB"
                     /locus_tag="ACGI57_002135"
     CDS             158..1072
                     /gene="murB"
                     /locus_tag="ACGI57_002135"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFG9717211.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            1076..1789
                     /locus_tag="ACGI57_002136"
     CDS             1076..1789
                     /locus_tag="ACGI57_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFG9717212.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            1820..2677
                     /gene="rapZ"
                     /locus_tag="ACGI57_002137"
     CDS             1820..2677
                     /gene="rapZ"
                     /locus_tag="ACGI57_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFG9717213.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            2689..3795
                     /gene="yvcK"
                     /locus_tag="ACGI57_002138"
     CDS             2689..3795
                     /gene="yvcK"
                     /locus_tag="ACGI57_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFG9717214.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            3864..4289
                     /locus_tag="ACGI57_002139"
     CDS             3864..4289
                     /locus_tag="ACGI57_002139"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFG9717215.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            4306..5253
                     /gene="whiA"
                     /locus_tag="ACGI57_002140"
     CDS             4306..5253
                     /gene="whiA"
                     /locus_tag="ACGI57_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFG9717216.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            5410..8979
                     /locus_tag="ACGI57_002141"
     CDS             5410..8979
                     /locus_tag="ACGI57_002141"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFG9717217.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            9188..10147
                     /gene="pfkA"
                     /locus_tag="ACGI57_002142"
     CDS             9188..10147
                     /gene="pfkA"
                     /locus_tag="ACGI57_002142"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFG9717218.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            10199..11959
                     /gene="pyk"
                     /locus_tag="ACGI57_002143"
     CDS             10199..11959
                     /gene="pyk"
                     /locus_tag="ACGI57_002143"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFG9717219.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            12134..13492
                     /gene="rlmD"
                     /locus_tag="ACGI57_002144"
     CDS             12134..13492
                     /gene="rlmD"
                     /locus_tag="ACGI57_002144"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFG9717220.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(13965..14603)
                     /locus_tag="ACGI57_002145"
     CDS             complement(13965..14603)
                     /locus_tag="ACGI57_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717221.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
BASE COUNT         5834 a         1597 c         2797 g         4455 t
ORIGIN      
        1 tatatttatt gaatatagtt tgtaattaag aagcttaaaa tataaaatcc atatgataag
       61 caaaatcata tattattgaa tatagttaat actatatatt aatgtaattt aaataataat
      121 gacagctaaa gttcagtata atttgggagg aaatacaatg aataatcaag atatatatat
      181 aaatttatta aatatattaa gtaaagagga tataaaagtc gatgaaccta tgaaaaaaca
      241 tatttcattt agggtaggag gaccagcaga tatattagtt agacctagaa ctgaagaaca
      301 attaaaaaat gtacttaagt tggtaaaaaa agagtctatt ccgtatttaa taattggaaa
      361 tggttcaaac attttaatta aagatggtgg aataagagga gtggtaatag aattagctga
      421 taatttcaat tcttatgaga taaatgatac taggatgact gctcagtcag gagctttatt
      481 atcagtttta gggaaagctc tccaaaagca agaattaaaa gggtttgagt ttgcatcagg
      541 aatacctggc acattgggag gagcattagc catgaatgct ggagcatatg gtggtgaaat
      601 gaaggatata gtgaagtcag taagactgat ggatatggaa ggcaatatat ttgaattatc
      661 aaatgaacag atggaatttg ggtatagaaa aagtataata tcgaaaaacg gatatatagc
      721 tttatcagca gaattagaat tacaagaagg aaattatgat gaaataaaaa gtttaatgga
      781 tgatttagcg acgagaagaa taactaaaca gccattaaat tttgctagtg caggaagtac
      841 atttaagaga ccaacaggat attttgcagg taaattaata gaagaaactg gattgagagg
      901 tcttacttta agaggagcac aagtatctga gaagcattgt ggatttgtag tcaatcaagg
      961 agaagcaagt gctaaagata ttttggattt aatatatgtt ataaaaagtg ctgtatatgc
     1021 caagtttggg gttatgttgg aagaagaagt caaaatactt ggagaggatt aacaaatgaa
     1081 aatactggct gattatcata cacatacatt atatagtcat ggcaaaggga ctattgagga
     1141 taatgtaaag gtagccattt ctaaaggaat aaaaacaata ggaatatctg accatagtta
     1201 taaacatatt gcatatggtg taaagaaaaa agatattctc aaaatgagag aagaagtaga
     1261 ttatttaaat gataagtaca ctgatataaa tatactttta ggtatggagt gtaacatatt
     1321 agatgataaa ggaaatatag atatggatga taaatttatt agagattttg attatattat
     1381 ggctggatat cattttggtt ctactccaac atcattaaga agcttattaa atcattgtaa
     1441 taactatatt tttaaaactg aaaaagcaaa agaatataat acaaaagctg taataaatgc
     1501 gataagtaat aatgatattt ttataattac tcatccagga gataaaggcg atgtgtatat
     1561 agaagaagta gcaaagacag caaaaaagac aaatactaga cttgagataa atagtagcca
     1621 ttcttttttg aatgtaaatc aattaaataa aataaaaggg ctagaaaata aatttataat
     1681 aggctcagat gcacattgtc cagaaagagt tggtgatttt gaactagcta tgaagactat
     1741 atctgactct aatctggata tatcgttagt agaaaacatt ataatataat aaacataaga
     1801 taaaagggag gagttagcaa tgaaatttgt tatagttaca ggtctttcag gatctggaaa
     1861 aagtgagaca atgagagctt tagaggatat gggattttat tgtgtagata atctacctcc
     1921 tgctttgata actaaatttg cagaattatg ttatcaacca aattcaagta ttgataaagt
     1981 tgctctaggg atagatataa gagggagaaa gttttttgaa gcgcttcatg aaagcttaaa
     2041 ttatttagaa aaagaaaatt atgaatatga aatggtatat ttggattgta atgatgatgt
     2101 attgctaaag agatataaaa tgacaagaag aaatcatcca ttggcaaaag atatgcagat
     2161 tccagaaggt ataaaaatgg aaagaaagat aatggaacct ttaaaggagt tatctacatg
     2221 tataattgat acaactaaca tgaaacctaa agaccttaag gaagaaataa agaaaatata
     2281 ttcttctggt gaagataatc caaacctaac tatttctgta gtatcttttg gatttaaaca
     2341 tggtatactt gctgatgcag atttagtatt tgatgtaaga tttcttccaa atccctatta
     2401 tgttgaagag ttaagagcta aaactggtga tgataaagaa gttagagatt atgttatgaa
     2461 ttctaaaatc agtgaagaat tttatgtgaa acttttagat atgattcatt ttttagtacc
     2521 tcaatatata gaggaaggaa aacaacatct agtaattggt gttggatgca caggtggaag
     2581 acataggtca gttactataa caaatcttat agcggaggat ttatctaata agggatatag
     2641 agttgtaaag aaacatagag actctatgtt gcgataatgg aggcaaacat ggctaaatta
     2701 cttattattg taggaatttt aggttggatt gcattaattg tatcgttatt tatatataaa
     2761 aaagcaaaaa atgaaaaagt aaaactattt caaaggtcta ttgttgaaaa tagagaacca
     2821 aatgtggttg tgataggtgg aggtactggt caatcggtat ttttaagagg tcttaaacac
     2881 actacgcaaa atataactgc aattgtaact gttgctgatg atggtggagg ttcaggtgta
     2941 ttgagagaag acttaggaat gctcccacca ggagacataa gaaactgttt attagcactt
     3001 gcaaatatag agccaacaat gaatgaagtt atgcaatata ggtttacaga agggctttta
     3061 aaaggtcaga gttttggaaa tttgtttttg gctgctatga atgggttgta tggaaacttt
     3121 gaaaaagctg tatataaatt aagtgaaatt tttgctataa caggaagagt tttaccagta
     3181 actttggaag atgtaaattt agttgccaaa ttagaaaatg gcaatataat aaatggagaa
     3241 tctagtatac ctgaagagtc aaaaattcaa aaaagctcta ttgataaaat ttttttaaat
     3301 ccaaaagatg taaaaccatt aaaggatgtt attgcatcta tttatgatgc tgacattata
     3361 attatgggtc ctggaagttt atatacaagt ataataccaa atttacttgt agaggggatt
     3421 gtagacgcta ttaaatcgtc tgtagctcca aaagtatata tagcaaatat aatgactcaa
     3481 ccaggtgaga cagaagggta caatgtatta gagcatgtta atgctatagt aaaacacact
     3541 gatgagaatt taatagatta tgtaatcgca aataatgaga tattgccaga agaaatgctt
     3601 gacttatata aacaagatgg agcagagcaa gtcttgttag ataaaaagca aaaagataaa
     3661 ttaaaagaga tgggtataaa aactgtagag aagaatttaa ttgaaataaa aaataattat
     3721 ataagacatg atgcaaagta tatatctaac atagttattg agttagccct aaatcataat
     3781 tacaacaaaa gttagtaaaa aaggatttta aaaaattgtg tagaaatcct tatcattatg
     3841 ttagttttaa ggagtgaaca tcgatgagag aagaggttag tgctggtggt gtagtcctgt
     3901 ttggcaatac gattctttta ctaaggaagt tcaatggaga ttgggtgtta cccaagggaa
     3961 aagtagaaga aggggaaaat aaccaagagg cagcattacg tgaagtaagt gaagaaactg
     4021 gagtaaaagc agatatattg aaatatttag gtgaaataca ctatacattc aaagaaaact
     4081 gggatgaaaa tagagcagtt cataaaacag ttttttggta tttgatgcaa gcaaagaata
     4141 tggatactat tccacaaaaa gaggaaggct ttattgatgc taaatttatc cacctagata
     4201 gagtagtaga cttagctagg tatgatgatg aaaaggagat aataaaggtt gccttacaag
     4261 aaataaaaaa aaggttaaag aaaaattagt taataggtga tagatatgtc tttttcaact
     4321 gaaaccaaaa atgaactagc aagagttata tctgggaatg aatgttgtaa tatagctgaa
     4381 ttgtcagctc tagttaaatc agggggaagt atacaaattg ttggatataa gaagctaaat
     4441 ctaaagataa caacagagtt aaattcaata gcccgtaaag tatttaaatt attaaaaaag
     4501 aattttaata tcaatactac aatctcagta aataagaatc aaatgctaaa gagaaataac
     4561 agttatgtat taatggtaac aagcgaaatg ggttcagaaa tgttattaaa aaagttaggt
     4621 atacttgaag ataaagaagg attttttact ataaacaaaa tttcagagaa cttaattaag
     4681 catgaaggat gtaagagagc tttcataaga ggagcttttc ttggtggagg ttctataagc
     4741 gacccagaaa agaactatca tatggaattt gtaactaata atgaggagtt tgcagattct
     4801 ctaaaagaac ttataaactc tttaggtttt aatagtaaaa ttgtggctag aaaaaataat
     4861 cacgttgttt atatcaaaga gagtgaacaa atatcagatt tgttgagtat tataggagga
     4921 caccacgctc ttttaagtct tcaaaatact aaaatagtaa aagaaatgag aaataatgta
     4981 aacaggatag taaattgtga gacagctaac ctttctaaaa cagtaaatgc agcagtaaga
     5041 caagtagaaa atattaagct catacaagag actataggaa ttagcagttt gccagaaaat
     5101 ttacaagaga tagctaagat tagaatcgag tatgaggata tgactctcaa agaacttgga
     5161 gagatgttag aaccaccaat aggaaaatct ggagtcaatc atagacttag aaaaatagaa
     5221 gaaattgcaa cagatttgaa aaaaaaatca tagcaataaa ttgaggggac aatctcagaa
     5281 taagcattaa tttataacat ataatggtat acgttgaagt taattcctat tttgagataa
     5341 aagtctctgt ttttgtttta ggacatatat ttgtggtaaa attaaaatta taaagcagga
     5401 ggtgatttaa tgaacaaaga ttttgctcat cttcatgtac atactgagta tagtttactt
     5461 gatgggtttt cgagggtgaa aaaacttata aaaagggcta aggaactaaa catgagctca
     5521 atagctataa ctgaccatgg ttgtatgttt ggtgttatag atttctacaa aacagctata
     5581 aaagaaggaa taaagccaat tatagggtgt gaagtctata cagctgcacg tggattgaga
     5641 gataaagacc caaactatga caaatatcaa ggacatttag ttctgttagc taagaatatg
     5701 gaaggatata aaaatttaat aaaaatagtt tcaacttcat acgtagaagg tttttactat
     5761 aagcctagag ttgacatgga agaacttaaa aaacataaca aagggattat agcactttct
     5821 gcatgccttg caggagatgt agcacgtgct ttaatgaata gaaattatga aaaagcaaaa
     5881 caatttgctc ttgactacag agacatattt ggagaagaaa atttcttttt ggagattcaa
     5941 gatcacaatt tacctgagca aaaagaagta aactctggtt tagtaaaatt atcaaaagag
     6001 actggtatac cactagttgc tactaatgat gttcactatg taaataaaga agattcgaaa
     6061 atacatgatg tacttatgtg tattcaaatg ggaaaaacat taaatgaccc aaatagaatg
     6121 agatttggta gtgatgagtt ttatttaaaa tcaagagaag aaatggaaga attatttcca
     6181 tatgcattag aggctattga caatactgtg aaaatagctg atatgtgtaa tgtggagttt
     6241 gattttaata ctatacatct tcctaagtat gatgttccag aaggatatac accagaaact
     6301 tatttaaggg agttgtgttt taatggatta aaagagagat atgataatcc tagtaatgag
     6361 atactagaga gattaaatta tgaattagat gtaattgaaa aaatgggtta tgtagagtat
     6421 tttttaattg tttgggattt tataaatttt tctaaagaaa ataatattat agtaggacca
     6481 ggtagaggta gtgcggctgg ttcgatagta gcatatacac ttaaaataac tgatatagac
     6541 ccaataaagt actctctatt atttgagcgt tttttaaatc cagaacgtat atccatgcca
     6601 gatatagata tagatttttg ttatgagaga agagaagaag ttatagatta tgtaaagaga
     6661 aaatatggtg atgaccatgt agcccagata ataacttttg gaacaatggg tgcaaaagct
     6721 gcaataagag atgttggaag ggtattggat ataggctaca ataaagttga caaggtagcc
     6781 aaggaaatac catttgcttt aggaatgaca atagataagg ccctagatac aaatcctaat
     6841 cttaaagaat tgtatgacca agacccggaa acaaaagaaa taataaatat atcaaaacag
     6901 atagagggaa tgctaagaca tgcttctact catgcagcag gagttgttat atcaaaaaat
     6961 cccgtagatg aatatgtacc tttgtacaaa catcaagatg caatcacaac acagtttact
     7021 atgacaacac ttgaagaact tgggctttta aagatggatt ttcttgggct aagaactctt
     7081 acagttataa gagatactct tgatttaata gaaaaaaata gagttataaa aaattacaca
     7141 gaaaaaatag acttttcaaa gatggattat gacgacccga aagtatatga aatgctttca
     7201 tcaggaaata cactaggggt gtttcaattg gagagtgctg gcatgagaag ttttatgaaa
     7261 cagctaaaac cagacaattt tgaagatatt gtagcgggaa tatccctatt tagaccaggt
     7321 ccaatggatt ctatacctgc atatataaaa aataaaagca acccaaaaga tgtaacatat
     7381 ctacatgaaa aattaaagcc tataatggaa gttacatatg gttgtttggt atatcaagag
     7441 caggttatgc aggtcgttag agatttaggt ggttatagtt acggacgaag tgacctagtt
     7501 agaagagcca tgagtaagaa aaagatggac gtgatggaag aagaaagaca atattttatt
     7561 catggtaaat ttgatgaaga tggtaatata gaaattgcag ggtgtatcag aaatggtgta
     7621 gaagaggaaa tagctaataa aatatttgat gatatgatag acttcgctag atatgcattt
     7681 aataaatcac atgcagctgc atatggggtt ttagcatatg agacagctta tctaaaaaca
     7741 tattatccag ttgaatttat ggcagcattg ataaccagtg taatgggaaa tactgataag
     7801 gtagttgagt atataagaga atgtaaagca ctagaaatag atgtattacc tccagatata
     7861 aacaaaagtt tttcaaagtt ttcagttgaa ggcgacaata taagatttgg tctagcagcc
     7921 gtaaaaaatg ttggcgtaaa tataataaac aatataatcg ctgaaagaga atctaatgga
     7981 ttatttgtag atttagtaga tttagtcaaa agattagacc aaaaagatac aaataagaga
     8041 gtaatagaaa gtctaataaa atgtggagct tttgataata taagtgaaaa tagagctagt
     8101 ctgatggctg gttatgaaag tttattagaa agtgtatcta tggatagaaa gaaaaatgtt
     8161 caaggtcaaa tttctttatt tgatgctttt ggagatagtg aggaagatag tgattttcaa
     8221 caaatataca gtttgcctaa aaggcaagag tacgaagaaa gagagagact taaccttgaa
     8281 aaagaagtct tgggaatgta tgtaagtgga catcctttat cacaatttga aaaagaacta
     8341 caagaaaaaa catctatgga taatggaaaa ttaaattcat taaaagagga agaagagtca
     8401 tacctacaaa tgaatgaaac agatgttata atgggtggaa tgattgtaac taaaactatt
     8461 aaaacaacaa aacgaaatga aattatggca tttattgaat tagaagattt gtatggtgct
     8521 atagaaataa tagtttttcc tcaattatta caaaagtata atgtcatttt gaatgaagat
     8581 aatataatat atgtaaaagg aacattaagt ataaaagaag gcgaaaatgc aaaacttata
     8641 gctagagaga taaaagatat aaatgatgat actgactttg cagatagaaa ctacaattca
     8701 ggaagaacta gaaatgtaga aaatattaag ccagaaagtg gtaaaaaatt gtatttgaaa
     8761 atagatagta tgtctgatac tgatacaact aataatattg tagaaatagc caaacaatat
     8821 ccaggtaatg acagtatata cttataccct atggatgaaa atataaatgg taaacgtaaa
     8881 gcatataaaa tgaccggggc attgacttgt atatgtgatg aatttataaa taacttagaa
     8941 gagttacttc ctaaagataa cataaaagta aaagcttgat tttttatata aaaatctaaa
     9001 tatattataa taatatgaaa aatattgtcc gttttgtgat ataatatcac aaaatggaca
     9061 tttaaggaca tttaattttt ttagtcttga tgggattaaa aagtgatata gggacaaaaa
     9121 aagtccatta gaatacaata acaatgatta taataaaaaa ttgacataaa atccaaggag
     9181 gtaaaaaatg aaaactatag gattactaac aagtggagga gatgctccag gaatgaatgc
     9241 tgcgattaga gcggttgtaa gatcagcaat atattatgga tgcaaagtat atggaatcaa
     9301 tagaggatat aagggattac tagaagaaga tttaactgaa atgaacttat cttctgttgg
     9361 agatattata cacaggggag gaactatatt aaaatcttcc agatgtgaag aatttaaaac
     9421 agaagaaggt agattaaaag cagtaaaaat tcttaaaaag tataaaattg attgcttagt
     9481 tgtaattggt ggagatggtt catttgcagg agcccaaaaa cttagtgact taggattccc
     9541 agctataggc ataccaggaa ctatagataa cgatttagca tatacagatt atactatagg
     9601 atttgatact gcaatgaata ctattataga tgctataggt aagataagag atacatcatc
     9661 atctcatgaa agagttaaca tagtagaagt tatgggaaga cactgtggag atttagcctt
     9721 atatgcaggt cttgcaggag gagcggaaac tataatagta ccagaagtag aaattacagt
     9781 agatgaagtt gctctaagat taaagactac tcaaaaaaga ggtaagagac atagtataat
     9841 cgttttagca gaaggagtcg gaagtgctag tgatttagaa aaggaactta aaaaagagtc
     9901 aggagcagat ttaagagtca ctgtattggg gcatgtccaa agaggaggaa gcccaactgt
     9961 atctgataga atactagcaa gtagacttgg agtaagagca gtagaattat tattagatgg
    10021 aaaatcagct agagttgtag gaataaaaga aaacaaaata attgatttag aaataagtga
    10081 agctttagct caaaagaaag tatttgataa agaagcttat gagatggcaa aaatattatc
    10141 tatataagtt ttagtattaa aaataaatct gtggaataat atctcaggag gagcaaatat
    10201 gttaaataat gtaaagaaga caaaaattgt ttgtacacta ggtccagcat cacaaagtga
    10261 ggaagtttta actcaactta tgcaaaatgg tctaaatgtg tgtagattta acttttctca
    10321 cggttcgcat gaagaacata aagaaagaat tgatatggca aagaaagtta gagaaaaatt
    10381 aaataagcca gttgctatat tattagatac taaaggtcca gaaattagaa caggtaactt
    10441 tgaagaccca gaggtatttt tagaagaagg gcaaaagttc actataacta tgaaagatat
    10501 tatggggact aaagaaatgt gtacagtaag ctataaaggt cttgctgaag acgttaaatc
    10561 aggagattct atactaatag atgatggttt agttggctta agagtaaaag aaataaatgg
    10621 agaagatata gtttgtgttg ttgaaaattc aggaatagtt aaaaatcata aaggagtaaa
    10681 tgtacctggt gttaagataa atttaccagc aataactcct aaagatataa gtgatataga
    10741 gtttggaata agtcaaggaa tagattatat agctgcttct tttgtaagaa aggcatctga
    10801 tgtactagca ataagagaag tattagagaa caataatgca actgatatac aaataatatc
    10861 taaaatagaa aaccaagaag gtgtagagaa cctagatgaa atattaaaag tttctgatgg
    10921 tataatggtg gctagaggtg acttaggtgt tgagatacca actgaagaga tgccaatagt
    10981 tcaaaaaatg atgataaaaa aatgtaatga attagcaaag ccagttgtta ctgccactca
    11041 aatgcttgat tctatgataa gaaatccaag accaacaaga gcagaagtta cagacgttgc
    11101 caatgctata tatgatggaa cagatgcgat aatgttatct ggagaaactg ctgctggaaa
    11161 atatcctgta gaggcagtaa aaatgatggc tacaatagca aaaagaacag aagagacttt
    11221 agattatgat agattactaa aggaaaatgg tactaacaat gttactgtaa cagatgcaat
    11281 aagccatgca acttgtacaa cagcagtaga tttaaatgca tcagctataa taacatctac
    11341 ttcttcagga tatactgcta gaatggtatc taaattcaga ccaaaatctc caataatagc
    11401 tactacaaat aatgaaaaga ctatgaataa attagctctt acatggggag tatatccagt
    11461 taagtcttct gttgcaggaa atacagatga agttatagaa aaggcaattg aggcagctag
    11521 acaagctgat tatatagata atggggaatt agtagttata actgctggag taccagtagg
    11581 agtaagtgga actactaatt taataaaggt tcatgtaata agcgaagaaa tcgtgcaagg
    11641 tataggtgta ggtactcaaa cagtagaagg taaagttcgt ataataaaga atggaaatgc
    11701 ttgtgtagac tttaatgaag gcgacatact tgtttctact gctactgatg ctgaaatgaa
    11761 caattatata gaaaaagcag gagctgtagt aactgagaat ggtggaatga catcacatac
    11821 agctattgta ggtataaatt tagatatacc agttattgtg tctgcagctg atataacaaa
    11881 tctagtaaaa gatggagaag ttgtaactgt ggatgcatca agaggcgtgg tatatagagg
    11941 ttcaactaga gttttataag aaaaataata aatttattta ctaaggggct ttataagctc
    12001 cttttttttt ggtaaaataa aatattgtag cttagtaaaa aatatagttt aattaatagc
    12061 aatattttaa tataaattta aagaaaaaag tttgtaaaga tttaaatata atagattgat
    12121 tggagggtga tttatgcttt cgaaaaacga gatgtatatt gtagatatag tagacattgg
    12181 tcaaggcgga gtaggagtag gaaagtacaa tggtattaca gtttttgtag agggcggatt
    12241 aatacatgat aaagttaaag ttaggattaa taaagtaaag aaaaattatg ctgtgggaga
    12301 tattgttgag ataatagaaa agtctccatt cagggtgaaa agagtgtgta gtgacaatct
    12361 tagggattgt ggagggtgtc agatacaaga cttagattat aataaacaac ttgaaatgaa
    12421 aacaaatgaa gttaaacaag tactttcgag aataggtaag ttagaggatg tagaagttca
    12481 tgacactata ggaatggata atccatatag atatagaaat aaagcacaat ttccaataca
    12541 aaagaataat ggtattccaa ttattggctt ctataaaaag aagactcatg atgtaatatc
    12601 gacagaaaaa tgcgtaatac aacatgagat aaatgataaa attgttaaaa taataaaaac
    12661 atatataagg gcctataagg taagcatata tgatgaaaag actcacacgg ggttgattag
    12721 gcatttagta acaaaaatag gttttacaac taaagaagta atggttgtat tagtagcaaa
    12781 tggaaacaaa cttccatatc ttaaagaatt agcttcagtg cttaaggaaa atatacctgg
    12841 atttaagact ttggttgtaa atgtaaatca aaaagatacg aatgctattc taggaagaga
    12901 aaatatagtt atatatggag atggaaaaat aaacgattat ataggagact tagtgtttga
    12961 gatttcacca ttatcatttt tccaagtaaa ccctgtccaa actaaagtat tgtatgataa
    13021 ggctcttgaa tatgcaaatt taaatgatac tgatactgtt tttgatattt actgtgggat
    13081 aggtacaata tctttatttt tggcacaaaa ggctaaaaag gtttatggaa tagaaattat
    13141 agatgatgcc ataaaagatg ctaagataaa tgctaaatta aacaatatac ataatgctga
    13201 attttatgtt ggaaaggcag aggaaattgt acctaagatg tatagagagg gtaagactgc
    13261 caatgtagtc gttgtagacc cacctagaaa gggatgtgac gaaaaggtat tagagactat
    13321 tgtttcaatg aatccggaaa gaattgtcta tgtttcttgt aatccttcta ctttagcaag
    13381 ggatttagat tatctaaatg ttcacggatt taaatgtgtt gaggcacaac ctgtagatat
    13441 gttccctcat tctacacatg tggaaactgt agctaagata atcaaacaat agtaatgttt
    13501 atatagatta tatactaata atatattgta tttgccaccg atttgccact gtaaagcgat
    13561 ttcggtggca aatttagtct acaatgttat caaaaatctc taaagtttta tttttaattc
    13621 tataaaactt attataattg taattttaaa agaaatagtc aatctacagt agccagattt
    13681 tatacaactc atttttttca tagagtaatt tacataatct atagttgaat attatttaat
    13741 atttttataa ttcattagca aatataggca ttacaaatgt tgataaacat gtgaaagcac
    13801 atatccactt ataattattt tttttaaaac cataaaatca cctcttcatt tgttgtatgt
    13861 ttttataaca aatgaagagg tgatttccag acaatttaaa aggtttttgt atattgacta
    13921 aaatttattt ttattattta taattcttaa taatacatat gtacctactg attccatatt
    13981 aaactgtata atttttgttc atcttttttt ataattaaaa ctacgtagtt ctcataattt
    14041 ctcttatata tatacttatc atctttttta acttctaact tatatttttt aaaatcttct
    14101 tcaattttat ttttattact tttattaatg ttaacagaat ctcttttaaa tttatcaata
    14161 tatttttcta tttcattaat attatctttg cttacaacta taccaccttt taaattaaaa
    14221 acttttttga aatttttttc agaaaatcct tgaataaaat aactttgtcc atctccatta
    14281 atactatctt gagtatcaaa tacttcaatt tctttgttag gtattggtat atcaaaattc
    14341 caagattcct ttagcattga aatattagct gtttttccta aaaaaagatt atataggata
    14401 cttcctaata acaaaacacc tattaatagt agtgtgatta atacaatttt tctctttttc
    14461 atttttcgtt cacaaccttt tgaaaaaaca tgatttaatt catcatttga aggcatgtct
    14521 tcaagaataa tttttgttgt gataggaatg ctttcttgaa acatatcatt tgtaatatca
    14581 aaatcttttc tttgattact caatatccat ttcttctaat atttcttttg attttttcta
    14641 gtacattgaa tactcttata tacattctcc tcttttaaaa tta
//
LOCUS       DAYAOX010000094        14176 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.094, whole genome shotgun sequence.
ACCESSION   DAYAOX010000094 DAYAOX010000000
VERSION     DAYAOX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.094"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     regulatory      133..237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            447..2921
                     /gene="gcvPA"
                     /locus_tag="ACGI57_002146"
     CDS             447..2921
                     /gene="gcvPA"
                     /locus_tag="ACGI57_002146"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HFG9717222.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            2921..4378
                     /gene="gcvPB"
                     /locus_tag="ACGI57_002147"
     CDS             2921..4378
                     /gene="gcvPB"
                     /locus_tag="ACGI57_002147"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HFG9717223.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            4801..7158
                     /locus_tag="ACGI57_002148"
     CDS             4801..7158
                     /locus_tag="ACGI57_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFG9717224.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(7244..7561)
                     /locus_tag="ACGI57_002149"
     CDS             complement(7244..7561)
                     /locus_tag="ACGI57_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFG9717225.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            complement(8145..9416)
                     /locus_tag="ACGI57_002150"
     CDS             complement(8145..9416)
                     /locus_tag="ACGI57_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9717226.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIMLEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     gene            9628..9867
                     /locus_tag="ACGI57_002151"
     CDS             9628..9867
                     /locus_tag="ACGI57_002151"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717227.1"
                     /translation="MECYLKRTLIIFSEVFLSISFKTRKYLFIVIFILVCPNLSEMIF
                     ILVLARRRTIRIDNIVSIYKYVGVLKLINYSILIL"
     gene            complement(11658..12209)
                     /locus_tag="ACGI57_002152"
     CDS             complement(11658..12209)
                     /locus_tag="ACGI57_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9717228.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            12400..13362
                     /locus_tag="ACGI57_002153"
     CDS             12400..13362
                     /locus_tag="ACGI57_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFG9717229.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            13657..>14176
                     /locus_tag="ACGI57_002154"
     CDS             13657..>14176
                     /locus_tag="ACGI57_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFG9717230.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIK"
BASE COUNT         5517 a         1551 c         2245 g         4863 t
ORIGIN      
        1 gaataatata aaagagaaat aattgataat aaaaacttaa caaaaaaatt aatacataaa
       61 aaaaaataaa tataatgtgt tgacatttaa ttgaaatatt catacaataa aattgaaaat
      121 aaatatttcc aaatgagatt agcgagagag tcctttatac gaaaaggcgc cgaagaagaa
      181 atttaagaaa tgccaatttt tagagaagct ttcaggcaaa aatatcgcta attgatggga
      241 ctctggagag acttaagtta agatactata agtcaccgaa gaagcaaaat aatattaaat
      301 tattaaatct ttcaggtata caaacaggga tatataggct aggattattt tccatgctga
      361 tatatctttt ttttatgaaa aagtacagga aataatatca aagttactta aatacagtag
      421 gtctaatttt ctagggaggg ttatatatga atgaattgaa aagagtatct ttgtacaata
      481 tacacaaaga gcttggagca aaacttgttg aatttgctgg ttgggaaatg cctttagaat
      541 atgagggtat aaataaagag catgagaagg tcagaaaaag tgcaggtata tttgatgtat
      601 ctcatatggg agaggtacag ataaagggag cagagtcaga aaaatttata caaaacctag
      661 ttacaaatga tataagcgca ttaaagataa atgatattat atatacacct atgtgttatg
      721 aaaatggtgg agtagttgat gatttattaa tatataaatt tggtgaagaa gattatttat
      781 tagtaataaa tgcaggtaat atagataaag atgtagcatg gataataaaa caaagtgaag
      841 gatacaatgt agatataaaa aatatatcta gtgaagtgag tcaattagca attcaaggac
      901 ctaaagcaga agaaattctt caaaaaatca cagatataga cttaaactca ataaaatttt
      961 ataagtcaat tccatcaaca aaagtatgtg gttgtccttg tttagtttca agaacaggat
     1021 atactgggga agatgggttt gagatatact gtaaaaataa atatgtagaa ataatttgga
     1081 atgaagttct aaaggttgga ggagaagata tatgtccagc tggtctaggt tgtagggata
     1141 ctttgagatt tgaagcagct ctacctctat atggacatga aataaatgaa cacatatctc
     1201 caattgaagg tggtttatct atctttgtga aaacaaataa agaatcattt attggcaaat
     1261 caattttatc aaaagaaaaa gaaagtggag ctaaaagaaa gttagtagga tttgaaatgc
     1321 aaggtaaagg aatgcctaga aatggatatg atataaggat tggtgataaa acagttggat
     1381 ttgtaacaac aggatgtgca tctccaacga caggaaaaat tttaggtatg ggaattatag
     1441 attctgaata tgcaaaagta ggaaatgaaa taggtatagc aatcagaaaa aaagttgttc
     1501 ctgctgtgat agttaagaaa cctttttata aaaaacaata taaaaaagat aatattatat
     1561 taaacaaaga aaataaattt tcatatatac ctgctacaag tgaagataaa tcaaaaatgt
     1621 taaaagtagt tggattaaat tcagttgatg aattattttc agatatacca gaagaagtaa
     1681 aattaaaaag agatttaaat ttggaaattg gaaaatctga attagaggta agcaaaatag
     1741 taaaaagatt gtcagaagaa aatttaagtt tagaagactt gacttgtttt cttggtgctg
     1801 gtgcatatga ccattatata ccatcaatta taaaacatat aacttcaaga tctgaatttt
     1861 atacagcata taccccatat caagctgaaa taagtcaggg aactttacaa gtagtttttg
     1921 aatttcaatc tatgattgca gaaattacag gtatggaaat tgcaaatgca tcaatgtatg
     1981 atggagcaac tgctgcaata gaagcatgta taatggcaat gaatcagact agaaaaagta
     2041 aaatagtagt gtcaaaaact attcatcctg aaactctgtc agtattaaga acttacttac
     2101 aatataaaga ttgtgagata gtagaaattg acttttgtaa tgaatatggg actacagata
     2161 tagaaaaatt aaaagcttct gtagacaaag atacagcatg tgttcttata caaactccta
     2221 atttctttgg aattattgaa gaaatggaag aaatagaaaa gataactcat gaaaataagg
     2281 ctatgttaat tatgagtgta gacccaatat cattaggagt tttaaaaaca ccaggtgaga
     2341 taggtgcaga tattgttgtt ggagaagctc aatcacttgg aaaccctctt aattttggag
     2401 gtccttatgt aggattttta gctagcaaat ctaaatatac tagaaaaatg ccaggaagaa
     2461 tagttgggca aagtttagat gtagaaggta aaattgcata tgtattaact ttacaaacta
     2521 gagaacagca tgtaagaaga gaaaaagcaa cttctaatat atgttcaaat caagctttaa
     2581 atgctcttgt agcttcaata tatatggcta ctatgggtaa agaaggattt aaagaggttg
     2641 gtatgcagtc tatgaaaaaa gcacattata cctacaataa acttgtacaa actggaaaat
     2701 ataagccaat atttaaaggt aagtttttca aagaatttgc tgtacaagga aatcttaata
     2761 tagaaactat aaatgataaa ctcttagaag aaaatatatt aggaggttat aatttagaat
     2821 acaattatcc agaattaaaa aattcaactt tgctttgtgt gactgagaaa agaagcaaag
     2881 aggaaataga taagttagtt ggaataatgg agggattata atgaaagaat acaatagttt
     2941 gttgatagat atatctaaaa aaggtaggaa agcatactca cttccaaagt tagatataga
     3001 tgatataaaa atagaaaata tgattgacca agatatggca agacaaagtg agttgaattt
     3061 gccagaagtt ggtgaattag aattagttag acattataca ttattatcaa ataaaaactt
     3121 tggagttgat acaggttttt atcctttggg ttcttgtact atgaaatata atcctaaaat
     3181 aaatgaagat atggcagcac ttccaaactt tactggaatg catccatacc aatcttcgga
     3241 tacagctcaa ggttctcttt ctttgatgta tgatttatca agaagacttg cagaaattac
     3301 aggtatggat gaagttacac tacaaccttc agcaggttct catggtgagt ttacagggct
     3361 tatgattata aaagcatatc atgaaaatag aggtgaccat aagagaacca aagtgataat
     3421 tccagattct gcacatggaa caaatccagc aagtgcagct atggcaaact ttgatgtgat
     3481 tcaaatagca tctgataaaa atggagctgt agacataaat gcattaaaag aggttttgaa
     3541 tgatgaagta gcagctctta tgcttactaa tccaagtact cttggtttat ttgaaaaaaa
     3601 tataaaagaa atagctactc ttgtacatga agcaggagga cttttgtact atgatggtgc
     3661 aaacatgaac gctattatgg ggataacaag acctggagat atggggtttg atgtggttca
     3721 tcttaatctt cataagactt tttcaactcc tcatggaggt ggaggtccag gagctggtcc
     3781 tgttggagtt aaaaaagaat tagtaccttt tttaccaata ccagtaattg aaagaaaaga
     3841 agataagtat gtactaaatt atgatagaga aaaatctatt gggaaaatta aaaatttcta
     3901 tggtaatttt ggagttcttg taagagctta cacatatata ctaactatgg gaagggatgg
     3961 acttaaagag gctagtgaaa tggcagtttt aaatgctaat tatataaagg aaagtataaa
     4021 agatgattat attttgccaa tagatacctt gtgtaagcat gagtttgtat taggtggatt
     4081 gccaagagga gaggccaata taaaaactat cgatattgca aaaagactac tagattatgg
     4141 ataccatcct ccaacaatgt actttcctct tattataaat gaggcactta tgatagaacc
     4201 tacagaaagt gagagtatag agacattaga tagttttata gaagctatga aaagtgttgc
     4261 taaagaggca aaggaagaac cagaactatt gaaaactgct ccacataata ctttggtcaa
     4321 aagagtagat gatgcaaggg cagtaaagaa acctatacta acatggtcac aaagataaat
     4381 agttaaaaag tagagattta aagtataaag agaattaaat ttgtaagaaa aagctatcta
     4441 acttaatcta gtttagatag cttttttgta tattagtatg tatggtattt tctatataaa
     4501 gttttaaatt atattagtgc ttatatagaa tattgatatg atgattatgt atagatatta
     4561 ataagaattg ttgaatttat cttgccttaa ctttatttac tcttttatat taagaaaaac
     4621 ttaactaaaa tatagcgaaa ttcataaaaa tttaggaaat cttaagaatt ataatattta
     4681 aatctttaga tttatatttt attatataaa ctatagaata ataaaataaa aaaattgaga
     4741 attttataat atatttgtag atttgagtat aaaaaagtgc taagttgaaa ggagtaaaat
     4801 atggataagg ttaatttaga aaggtatagt tgtgatataa atgttggttt aaataaaaat
     4861 caacttcaaa gtagaataga tgataattta attaatgcat ttgataatgg aaaaaccaaa
     4921 acatataaac aaatttttaa agacaatata tttacacttt ttaatctcat aaatattgtt
     4981 ttagtctgtt tattagtttc agttggttct tttaaaaata ccttatttat atttattgct
     5041 gtaattaata ctattatagg agtaatacaa gagataagag ctaaaagatt attggacaat
     5101 ctatctgtta tagtttcaaa taaggtatct gtcataagag aaaaagagaa aaaagagatt
     5161 gatgtacaag aattagtatt agatgatatt atgattttaa aaacaggtaa tcaaatttgt
     5221 gcagattcaa aagttttaaa tggaaaatta gaggtaaatg agtctttggt tacaggtgaa
     5281 tctgatatta ttataaaaaa taaaggcgat tttttatatt ctggaagttt tgtagtttct
     5341 ggagaagctt ttgtaagagt tgaaaatata ggaaaagata actatgcaaa taagatagct
     5401 aatgatgcaa agatttctaa aaaacataat tctcaactta gaaattcatt aaatgcaata
     5461 ttaaaaattg taggtataat aataattcct ttaggaatta tattgtttgc aaaacaacat
     5521 tttggaaatg gagattctat agctacatct gtagtaggta cagtggcagc tgtaaatggt
     5581 atgataccag agggattaac attacttacg agcattgctc tagctgtagg aactataaaa
     5641 cttgctagtc ataaaacttt agtgcaagaa ctgtactgtg tagagactct tgctagagtt
     5701 gatacactat gtcttgataa gacaggtaca attacagaag gtaaaatgca agttgatgat
     5761 atagtaatgt tggaagaagt ggatattaat gaaataatgg ggaatatatg taattctctc
     5821 aaagatgata atgcaacact aaatgcaata agagataagt ataatgtttt agatacgtat
     5881 aaagctaaga atttaatacc attttcttca gaaagaaaat acagtggggt tacctttgaa
     5941 aataaaggta catatttatt aggtgcattt gaatttatat ttaaggaaaa aaataaaaaa
     6001 cttaggatag aagttgaaag atattctaaa aaaggaaaca gagtaatagt tttagctcat
     6061 agtgattctt atatggaaga taataatatt cctgaagata taaagccact agcttttata
     6121 ttgatattgg ataaaattag agatgaagct aaggaaactt tagaattttt ttacaatgaa
     6181 ggtgtagata taaaaattat atctggtgac aatcctataa cagtaagtga ggtagctaga
     6241 aaagcaggtc ttaaaacagc tagtaatttt attgatgcaa caacacttaa aaatgatagt
     6301 gatatataca aagcagttga taaatacagt gtttttggaa gggtaagccc acaacagaaa
     6361 aaacaaatga ttttagcatt aaaaaaacaa aatcatacag tagctatgac tggagatggt
     6421 gtaaatgatg tgctagcact taaagaagcg gattgtagca ttgccatggc atctggaagt
     6481 gatgttacta agaatgtgtc taatttagtc ttattagatt caaactttgc atcaatgcca
     6541 aaggttgtta tggagggaag aaaagtaatt aataacattc aaagagcatc ttctttgttt
     6601 ttagtaaaga caattttttc attcttcttg tcgttattaa cattattctt cttcagtaga
     6661 cagtatccat ttgttcctat acaattatca ctcattgggg ctgtaacagt gggtatacca
     6721 tcattcttct tagctcttga agcaaacaaa agtagaattt caggaaattt tttactcaat
     6781 atattaaaaa atgctttacc aggagcacta tgtgtgacag taaatgtaat tattgtttac
     6841 aatatagttg aatatttagg ttatagttca agtgtatttt ccacaatgtg tgctatatta
     6901 actggtgtat gtggtctttt aatattaaga caacaatgtt tgccatttaa taaagaaaga
     6961 ttatttttaa tagtaagtat gactattgca tttgttattg ggattctatt tttaggtgga
     7021 ttattctcat ttgtagcatt gagtagagaa ttaattctca taactatggc attagcaata
     7081 ttaatgccaa ttatgataaa agttatgtta tttgtcttag atgagatatt agagacaatt
     7141 gttccagatt taaattaatt ttaaaatgat ataaataaga gcagttttag ttgaactgct
     7201 ctttttagat gtgtttaatt ttatgtaaga atatgtattt ttattaatca gctatatatg
     7261 agcaacagta gtcaacaact tcattaacct ttaagtcaaa acttgaatca ctaggaacat
     7321 taaatttttg cccttcttta taagttacaa actcattgct tccaggcagt tttacatcca
     7381 tacttccagc aagcatctcc attatttctt cttctctagt agaaaaagtg tattctcctg
     7441 gcaacattaa tcctaaagtt tttctttctc cattaggcag tattattact ctgctactta
     7501 ccttaccatc aaaataaacg tttgccttct ttactgcagt aacattctta aattcactca
     7561 ttagataaaa cctccaaaat tatttgtaca tatatattat ataaaaataa tgtaaaaaat
     7621 gctatatatt tacaaaaaaa tttagtattt attaaaaaaa ttaataagaa taaaaaagag
     7681 atgaaattgc ttaaattgat aaaaaatata aaataaattg caaagtaagc aagaaaagaa
     7741 taagttgaag agttatatat tataaatatt aaaaatttta gtattaataa aaaagatatt
     7801 acattaatat gaaaatatag gagattagaa gtgtttgata attacagtac atgttagata
     7861 tatcattgta aattaacata gctttgaata taataatgtt cagaaggcaa tatttttgag
     7921 gtctgaatcc aataataata tagtcttatg attgtagata tcaaaaaatg taagttacta
     7981 ggaatacgtt ttgtagattt atatatgatt aataactaaa gctataaata aacaaaataa
     8041 aaagagtatg tttcaaccta tagcttttag atttaaaata tactctttta ttaattatag
     8101 ttgtataaat taaccataat atataattaa aatttttatt ataattatat attaggttct
     8161 tcaattagtt ttccacatac atatctatga atgatatttc tatcatcacc aacatatata
     8221 aatctttcaa gtctttcaag tatagaataa ttgtcatgat ttaaatttga atcatcaata
     8281 atcaatgcat caaaatcata accttcttca aagctaccaa cttttccaaa gaaacttcca
     8341 ccacttttgg ttgcaatata aaaagcctca gataaagata agaaattata cttcttacca
     8401 gagttaaccc aatataattt agataattgt attgcagata ccattgcttt aaatattgac
     8461 aatgtatgtc caccacttat atctgatcca agtgctaatc taatattatt atcgattaat
     8521 tttctgatag gcatagcacc actaccaaca ttaaagttag aagctggaca gtgaactgcc
     8581 ataacattat tttctctcat taaatctatt tcatcctgag aacaatgaac acaatgagcc
     8641 attaaagtag gagtttgacc aaataaatta tatctattat atgcatctcc atagaattta
     8701 gattcaggtt ctagacttct aacccattca atctcatcat aattttcaga taagtgtgat
     8761 tgaataggaa tattatattt taaacataaa tctcctaaac cttttaaaag ttcacttgta
     8821 cagcttggta taaatctagg tgttattatt ggttttacaa tagaatctgt atcactatac
     8881 ttatttataa tttcttctgt atcagtaata gaatcctgag tgttttctaa taaagtatca
     8941 gggcaattca tattcatatt tactttacca acgtatgctc caagaccaac ttctttgaat
     9001 atatccataa gtttcataga ggcttcttta tgtactgttg caaacactgc tattcttgta
     9061 gttccagatt tccaaagttc ttttacaaat tttttataca tttttgttgc atattctata
     9121 tctttataat ttgattcttc tggaaatgta taagtttcaa gccatggcaa aagctcttta
     9181 tccatagcca ttccaagatt tttaaattga gaggcatggg catggaggtc attcatacca
     9241 ggtattataa gcttatcagt gtaatctact acttttaggt ttttgtattc cttaggtatc
     9301 tctttaaata ttccctttac tttacctttt tccagcataa tataactatt ttcaaatact
     9361 gtgaaagttt ctgaagtttt tgtaaaaatt atatttcctt ttagtacttt taacatatag
     9421 gtctccttag ttttaaattt gttttaatat atgtaggtaa aattttgttt tacaaatcaa
     9481 aattttacca catgtattat atcaaatcta aactataaat actattagat aatagcaaat
     9541 attagtgaaa tacagttgtt tactattatt atatataact gacatttaag tgacatttaa
     9601 gaaaaatata atgcctactt acataaaatg gaatgttatt taaagagaac tttgattata
     9661 ttttcagaag tttttttatc catatcgttt aaaacaagaa aatatctatt catagttatt
     9721 tttatattag tatgtcctaa tctttcagag atgattttta tattagttct agctagaaga
     9781 agaactatta gaatagataa tatagtaagt atttacaaat atgtaggtgt tcttaaattg
     9841 ataaattatt ccattttaat tttatagttt gaattttatg atataataaa aatatataaa
     9901 ttttgcagtg agcgatattt ttgataaagt agggtttaac agttgcaatg taagggattg
     9961 agggtgtatg ataaatgtta tcaattgcac tactcatggt tcactgcaaa tttgagagag
    10021 ttgtatgtgt gtaagtactg aaaatactta gtttattttg gggttttata ttaactaagt
    10081 ggtatgtaaa taaataatgt gttttatcat cataactcct gctaaacatg ttttatatta
    10141 actatgtggt atgtaaatct tgttaatgca aataaacaac atagcactat taatagtttt
    10201 atattaacta tgcggtatgt aaatagggtt tctgataaaa tcttcaaaca tgtaaaatat
    10261 gtgttttata ttaactatga ggtatgtaaa tcttatactt agttagaact atatatcgac
    10321 acaaatatgc tttatattaa ctatatggta tataaattgc aatttttatt cgttgtccaa
    10381 tctctttgaa atttgtttta tattaactaa gtggtatgta aatttgtaac aagtataggt
    10441 aaaatacccc aataatttat acagcatttt atcctttaaa atataattat tttttatcat
    10501 ttgtagtaaa taattaccag ataacattga ctttagtttt aatgattaaa atataaaagt
    10561 agaataatta taaaaagtat tgaaaaattt ataaatatat ataataaaac ttaatgacaa
    10621 gatattagat ataaaaaata attaccttat aaatagattg aaatttatga atattcatac
    10681 tataatttaa atataaggag atgccctttg aaaataaaaa ttaaaaaata tttaatgcta
    10741 ctacaatagg aactagaact acacttaata aatatacaga aattagattt ggctcaatat
    10801 aaaatacaaa tatagaattt aggtgttttt tatgaaaaaa tttttatatg ctttatatag
    10861 tttcattgtt attatagcta attttagatt aaaagaaaaa aattataact ttattttatt
    10921 agcaatcttt tttatattgc tgttaattca aaatggagtt ttaaaaacag gttatcaaaa
    10981 tagagctaca ttttatcaag ataattattt taatagagag gataaaaatg ataagataaa
    11041 atttatgact ataaagattt ttatattttt gtcattacca ttatgtttta atatactatt
    11101 caattattta acctcttaaa ttatatttag atataggagt cttatgataa aagtaagctt
    11161 atgtgttatt tgtagtaaat ataaagtgca tatagtgata aaacaaaagt ttcatataaa
    11221 tgaagaaaaa aatataaaat aatttattta actacttaaa aataaactaa aagggataat
    11281 aaaatagctt ttaggttatg gtaataaagg tatttaaaaa gagtgcataa gcactccaat
    11341 tgtgagaaat acatacacaa atcattatta caacaatgaa aaatgtacat ataagagacg
    11401 ttttgtattt gttttaagtg ataatatttt aatactaact aaataattat cacaatagta
    11461 aaaaagaaaa aggagtattt tgtactccat ttgtcaaaaa tataaaactt ttatataagc
    11521 ttattataac ataattgata aaagtgtaaa gatatataat gaatttataa agaaaaataa
    11581 aataaaaaag ataaatccta atcaaaggat ttatcttttt gtatagaaaa taattattct
    11641 attattaaat ggcatggcta aactggataa taatttatta ttactgcttt caatgttttt
    11701 tctccttgat tatggtatgt atgaattgat tcagctgaaa aacaaataga gtcattttct
    11761 ctaagttgat aaatagagtc atttacttct aattttaatt gaccttctat aatcataata
    11821 tattcctgtg atttttcaga atgaccaaca gatgtataac tatgaccttc ttctaattcc
    11881 atttgaaata attcgaagtt tctagttggt gtattgggat aataacaata aagacgataa
    11941 tctttatttt ctgaaacttg tacatctata tcagtttttg atacaataaa agtttcattt
    12001 tgaggttgtt ccagtattgc agtatatgga acatttagtc cacttgcaat tttccaaatt
    12061 gtgtttactg ttgggtttga gtctcccttt tcaatttgcg aaagcattac tttacttacg
    12121 ccagataatt cagcaagttg acctagactt aagttacgtt cattacgtaa tctatttaaa
    12181 tttttagcaa taacagtatt aatactcata gtagctcctt aaaatgtaaa ttattgacaa
    12241 atataattta cgaatgtata atttatttaa cattatttat aatttacaaa tgtataatat
    12301 aatgaacgtt aaatacactg tacatccgta atataaatta tactgcataa agagttgttt
    12361 ttcaacggat gatagttgag agattaaggg ggaattgtaa tgcgtgtact taataatgtt
    12421 acagaggcta taggcaatac tcatatgtta catttatcca aaatagcaga tgaatttgga
    12481 gttcaaggta atatttatgc aaagatggaa tacttaaatc cgggatttag taaaaaggac
    12541 cgtgtagcat tacaaatgat taccgaagca gaaaatgcag gtttattagt tccaggtcaa
    12601 gcagttgtgg aattaacaag tggtaatact ggaacaggac tagcaatagc ttgtgcatgt
    12661 aaaggatata aatttatagc gtgtatgtca aaaggaaatt ctatggaaag agcaagaatg
    12721 atgaaagctt taggtgctga agtagttctt gtagaccaaa tgcctaattc agttcatgga
    12781 caggtatctg gtgaagattt ggcacttgta gaaattgagg ctcaaaaaat agcaaaagaa
    12841 agaaatgcat ttagagcaga ccaatttaat ttagaagctt gtaatcatgc acatgaaaag
    12901 tatacagcag aagaaatgtg ggaacaattg gatggaaata ttgatgtatt tgtagatttt
    12961 cttggaagtg gtagtacttt tgcaggatgt tcaaaaacac ttaaaaaaca taattcttct
    13021 atcaaatgtt atgttgttga acctgaaaca gcagcaattt attcaggtaa agaaataact
    13081 gaccaagggc ataaaataca aggtggagga tattctatgg atttgccatt agtagacaag
    13141 aaacttattg atggttatat tcaagttaca gacgaggagg caactgatgt agcaagaaaa
    13201 ttagctaaat tagaaggtgt atttgcagga ttttcttcag gggcaaatgt atgtgcagca
    13261 attaagttgt taaagagcag tgaaaaagga aaaaatatag ttttaactct taatgatagt
    13321 ggtttaaaat atttaagtac tgatttatat gaagaattgt aataatagca caatagttat
    13381 gcaatgtttt ttaaattaag tatatagatt taaattataa aaagtatctc aatgtaattt
    13441 ttgaaagtta taaaggagat acttttttat tttgtaaata tggagtatcc tacttataag
    13501 tattaagcta tatagatatt gatgattagc tatgcaataa aaaagagtat gtgtttttgc
    13561 atttatacat cataacttgg aataatacat attaatgttc taaatgtatt taaaacatat
    13621 gataaaatta gaacaaatag atacaaggag ataaaaatgc aaaagagaat tttaagaaat
    13681 tttatattta cgggtaaaaa tattttaagt aaacttgttt ttataataaa atataatttt
    13741 agtacattaa ttttatttga attatttcat aaaggattag ccttattttt tatatggcca
    13801 tctattagat atatatttga tattttaata aaaagtttac atatagtcta cctaaatatg
    13861 gataatttta taaaagtttt gaccaatccc atgtcaatag ttctgataat actatcagtt
    13921 ataatactag ctttttatgc attttttgaa tttacctctg taattatttg ttttaataaa
    13981 tcgataaaat atgaaaaaat agggttattt gaactcttta aaatatcatt taagaactct
    14041 ataaaattgt tatatcctaa aaatatacta ttatttatat ttgtgatatt aataattcca
    14101 ttagtaaata cagttttaat ttcagggttt ataggaaaaa tacaaatacc agagtacata
    14161 ctagattata taaagt
//
LOCUS       DAYAOX010000095        14154 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.095, whole genome shotgun sequence.
ACCESSION   DAYAOX010000095 DAYAOX010000000
VERSION     DAYAOX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.095"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            190..1350
                     /locus_tag="ACGI57_002155"
     CDS             190..1350
                     /locus_tag="ACGI57_002155"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFG9717231.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1352..2482
                     /gene="glgD"
                     /locus_tag="ACGI57_002156"
     CDS             1352..2482
                     /gene="glgD"
                     /locus_tag="ACGI57_002156"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HFG9717232.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2511..3953
                     /gene="glgA"
                     /locus_tag="ACGI57_002157"
     CDS             2511..3953
                     /gene="glgA"
                     /locus_tag="ACGI57_002157"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFG9717233.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            3967..6387
                     /locus_tag="ACGI57_002158"
     CDS             3967..6387
                     /locus_tag="ACGI57_002158"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HFG9717234.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRVLL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            6392..8257
                     /locus_tag="ACGI57_002159"
     CDS             6392..8257
                     /locus_tag="ACGI57_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFG9717235.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIRIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            8502..9155
                     /locus_tag="ACGI57_002160"
     CDS             8502..9155
                     /locus_tag="ACGI57_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9717236.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPMEEMKKEKLL"
     gene            9251..9850
                     /locus_tag="ACGI57_002161"
     CDS             9251..9850
                     /locus_tag="ACGI57_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFG9717237.1"
                     /translation="MYPNKEIAIEELEVAGKMNPGPWIKHSYNVANVAKLIAEQCENL
                     NSEKAFVCGLLHDIGRRTGISSVRHIIDGYDYVISKGWDEVARVCLTHSFPVKDIEAD
                     IGNKDISKEQYDFINHYLNNLEYNDYDKLIILCDALADANGFCILEKRFVDTTRRYGI
                     YPFSVERWNKTYEYKEYFEKIINKSIYTLLPNIEKSIYY"
     gene            10440..11915
                     /locus_tag="ACGI57_002162"
     CDS             10440..11915
                     /locus_tag="ACGI57_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFG9717238.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            12002..12415
                     /gene="speD"
                     /locus_tag="ACGI57_002163"
     CDS             12002..12415
                     /gene="speD"
                     /locus_tag="ACGI57_002163"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFG9717239.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            12445..13296
                     /gene="speE"
                     /locus_tag="ACGI57_002164"
     CDS             12445..13296
                     /gene="speE"
                     /locus_tag="ACGI57_002164"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFG9717240.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            13286..>14154
                     /gene="speB"
                     /locus_tag="ACGI57_002165"
     CDS             13286..>14154
                     /gene="speB"
                     /locus_tag="ACGI57_002165"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFG9717241.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISD"
BASE COUNT         5692 a         1425 c         2574 g         4463 t
ORIGIN      
        1 aaataatgta tacgtcggta agtccattat ggggtataca ttatatcaat tgtatagttg
       61 atattatttt atttttatta aggtattatt taagcaacat ttatacacga catttattga
      121 ctgataaagt ttataaaagt aaaaatgcaa ttataaatat taattactag atgaggggag
      181 tgtcattaaa tgaaaaaaga gatgttagct atgattttgg caggagggca aggttcaaga
      241 ttaggtgttt ttacaaaaag aattgccaaa ccagctgtat catttggagg taagtataga
      301 attatagatt ttgtgttaag caattgttca aactctggta ttgatactgt aggtgtttta
      361 actcaatata gaccgttgat attaaattca catataggca tgggaagtca ttgggatttg
      421 gacagaataa atggtggtgt ctatgtttta caaccattta tgaatgaaaa agaaggaaat
      481 tggtataatg gtacagcaca tgcgatatat caaaatatgg actttgtaga tacctataat
      541 ccagaatatg tattgatatt atctggtgac catatataca agatggatta tagcaaaatg
      601 ttaaaatttc ataaggaaaa aggctctaaa gctacaatag cagtgattga agttccttgg
      661 gatgaagctt caagatttgg gataatgaat acaaatgaag attcaagtat ctatgaattt
      721 gaagaaaaac caagtgaacc taagagcaat ttggcatcta tgggagtata catatttgat
      781 tggaaaatgt tgagaaatta ttttaaggaa gcagaaaaaa atccagaaat aaattatgat
      841 gattttggaa aaaatttaat acctaaaatg ttagaagata atgttggaat gtatgcttat
      901 ccatttaaag gatactggag agatgtagga actatacaaa gtctatggga tgctaatatg
      961 gatataataa aatctccaga aacacttgat ttagcagacc ctaagtggaa gatatataca
     1021 aatactatgg caatgcctcc acaatatata ggtaaaaatg caaatgttca tagatcaatg
     1081 atagctgatg gttgtagaat tttaggtgaa gtaggtaact ctgtattatc acatggagtt
     1141 gttgttggta aaggaagtaa agtaattgat tcagtaataa tgccaaatgt tgtaataggt
     1201 gaaaatgtaa ctatagaaaa agctatgata ggtgagtgtg caactataaa tgataatgtt
     1261 cagataaaga atgtaaataa tgaaataaat gtagtttctg agtatgaaaa tatcgaacct
     1321 agatgcgtat taatagaagg aggtctttaa gatgagaaat gaatgtttag ggataataaa
     1381 tttaaacaaa aaaggagacc cagcaataaa caaacttaac tatggaagac caatagcatc
     1441 tacaccaata gctggaagat atagaataat agactttgct ttatctaata tgataaattc
     1501 aggaatcact aaagttggaa tattcgcaaa agaaaaatac agatcattaa cagaccatat
     1561 aggtagtggt aaagattggg atttaagtag aaaaaaaggt ggcctatcaa tttttagtcc
     1621 agaaaacact aagtatagaa atacttactc acatagagaa ggtgatatat atactatctt
     1681 agctaatcta gattatatag aaaagagtga agaagaatat attttaatag ctccaagcta
     1741 tatgctatgc aatttaaatt attctcaagc attggaatac cataaaaaat ctcataatga
     1801 tataactata atatataaaa atgtaaataa tgcaaataaa gattttgctg gtaacttaac
     1861 tctaaattta gatagtaaca atagggtaat aaatgtagga aataatttag gtaagttccc
     1921 tagagcaaac atatgtatgg agacatatat tatgaagcgt gaagattttg ttgaatgtat
     1981 atataacatt gtaaataaag gcaattattg ttatttagaa gaatttatta ttgaagaagc
     2041 agaaaacatg aaaataggag cctatgaata tacaggatat ctaaaatgtg taaattctgt
     2101 tgaatcatat tttgaaatga gcaaagattt attaaattta gaagttgcag atgaattatt
     2161 atattctgaa agaaagatat ttacaaaaga aaagaatgaa tcacctacaa tttatacaga
     2221 tagtgctaaa gtagaaaata gctttatagc atctggttgt ttaatagaag gtactgtaaa
     2281 agatagtatc atatttagaa aggtaaatgt tgaaaaagga acagttatag agaactcaat
     2341 agtaatgcaa aattgtgtaa taaagtcaaa tgcaaaacta tataatgcaa tattagataa
     2401 aaatacaagt gtatcaaatg ggaaagaatt aagaggagat gaaaaatatc ctcttgtagt
     2461 ggaaaagaat acaaatatat aaataaattt tttagaaggg ggaatgacta atgaaggttt
     2521 tttatgtaac agcagagtgt tggccctttg caaaaactgg aggattagga gatgtttctt
     2581 atgcattgcc taaagaatta aaaaaagaag gtgtagatgt aagggtaata atgcccaaat
     2641 attcaactat accaagttat ttaaaagatc aactaaaaga aatagctgtg tttagtgtac
     2701 gtgttggatg gagaaatcaa tactgtggtc ttttagaaat ggaattggat ggtgtaaaat
     2761 tttactttat agataatgaa ttttatttta gaagagaaga tgaaagaaag agtatctatg
     2821 gatatggaga tgatgcagaa agatatactt tctttacaga tgcagtttta gaagctataa
     2881 gtagaattga tttttatcca gatgtaatac atataaatga ttggcataca gggatgttac
     2941 cattaattct aaaagaaaga tatgcaaccc ttgaggggta taaaaatata aaaactatgt
     3001 atactataca taatctccaa tatcaaggtg tatttgataa gcatgtttta tatgatatat
     3061 tggacttacc acaaaaatac tttgataatg gagatataga gtattatggc tcaataaatt
     3121 ttatgaaagc tggtataaat tttgcagata aaattataac tgttagtcct acttatgcaa
     3181 atgaaataca aacatctttt tatggcgaac aattagatgg attacttaga aaagagtcag
     3241 gaaaattaaa aggtatatta aatggtatag attatgattt aaatgaccct gcaaaagata
     3301 aagatatatt tgttcattat gatgtagact caattgacaa aaaagtagaa aataaattga
     3361 gattacaaga tatattggga ctaaaaaaag attcatctat tccgttgata ggtatagttt
     3421 cgagattagt ttctcaaaaa ggatttgatt taatagcata catgatgcca gaacttatga
     3481 gagaagattt acagattgtc gttttaggaa ctggagaaca tcaatatcaa tcaatgttca
     3541 actattacga ttctaatttt tcagataaag tatcagctag aattactttt aatgcatcat
     3601 tggctcaaca gatatatgct gcaagtgata tgttcttaat gccatcatta tttgagcctt
     3661 gtggtatagg tcaaatgtta gctatgagat atggttcttt accaatagtt agagagactg
     3721 gagggcttag agatacagta acaccttata ataaatttac tggagaaggc aatggattta
     3781 gttttaaaaa ttacaatgct catgagatgt ttttctgttt gaaaaatgca atcaaagtat
     3841 ttaaggacaa agaaaaatgg attaaattag ttgaaaatgc aatgaaaaca gataatagtt
     3901 ggaaaaaatc agctaaggag tatatagaaa catatagaga catatgtgat taaaggagtg
     3961 gtttttttgg taacaataag caaaaagaaa ttcaagcaaa aatttgaagt aaaaatgtat
     4021 agcttatatg cacaatctat caaggaagct actgatgaac aattgttaaa tgtattatgt
     4081 agtctattga aagatgagat agcaaaaaaa tgggttgcta caaagttaga taagaaaaaa
     4141 gaagtttatt attttagttt agaattttta atagggagac agttaaaatc taatttatta
     4201 aatttaaata ttgaagaaga agttagagaa ggtttatctg aattaggaat caacttggat
     4261 gatttaattg aagctgaagt agacccagcc ttaggaaatg gtggacttgg tagattagca
     4321 gcttgctttt tagattctat ggcatctttg aacataagtg gtcaaggata tggaataagg
     4381 tataaatatg gtctgtttga acaaaaattt gtaaatggtt atcaggtaga agtgcctgat
     4441 aactggctta cagaaggacg atatgcatgg gaaactgtaa gaccaaatga agcaacaatg
     4501 gtgaagtttg gtggtgaggt agaattaatc aaggaaggta gtcatttaaa agtaattcat
     4561 aaaaactacc ttcctgtaat ggcaatgcct tatgatatac caataatcgg ataccaaaac
     4621 caatgtataa atactttaag attgtttaag agtgaaatac caaagagaga ttttggtgaa
     4681 cttacctcaa atgctctaaa ttattctggt agctatgaag aggctttaaa acataagtat
     4741 tatactgagg aaatatcaca ggtattatat cctgatgatt caaactatgc aggtaagttg
     4801 ttgaggttga aacaagaata cttttttgta agtgcaggga tacaagatat aataagaaaa
     4861 tataaaaaga acaaattaaa tattaataat ttattcgata aggtagcaat tcatataaat
     4921 gatacacatc ctaccttatg cataccagaa cttatgagag ttttgcttga tgaagaaaat
     4981 ttatcttggg atgaagcatg ggaaataact aaaaaaacag tgtcctatac taatcatact
     5041 ataatgtcag aagcaatgga aaaatggcct gtaagtatga tgaaagaact attaccaaga
     5101 atttatatga taatagaaga aataaataga agatatgtag aggaattaaa taataaagga
     5161 tatgaccaag atagaataaa aagaatgagt ataatagact gtgacaatat caatatggca
     5221 aacttatgta ttgtcacaag tcatagtgta aatggagttg ctaagcttca tactcatatt
     5281 cttgaaacag aggtattaaa agatttctat caagatgaac caaataaatt taataataag
     5341 actaatggaa ttgcacatag gcgatggctt ataagttcaa atcctcaact tagcaattta
     5401 attacagatt taattggtga ttcatggaag aaagatacat tacaattaaa aaatattgaa
     5461 aaatttaaaa atgattcctc tgttttacaa agacttgatg atataaaata caataataaa
     5521 gcaaatctag caaagtttat aaaagataaa tacgatttaa atgtagaccc aagttcgata
     5581 tttgatgttc aagttaagag attgcatgcg tataaaagac agttactgaa tatatttaat
     5641 gtacttcata tgtatcatga attacttgat aatcctaatt tgaatcttga cccaagaact
     5701 tttatatttg gcgcaaaagc agcaccagga tattatttgg ctaaatgtat aattaaattt
     5761 ataaattctg ttgcaagtac tataaataat gatgttagag taaaggacaa gttaaaagta
     5821 gtatttttag agaattatgg agtatcatta gcagaaataa taataccagc agcaaatgtt
     5881 agtgagcaaa tttcaactac aactaaggaa gcatctggaa caagtaatat gaagtttatg
     5941 atgaatggtg ctataacttt ggctacactt gacggtgcaa atgtagagat atgtgaacag
     6001 gttggtaaag aaaatatgtt tttatttgga cttagtgcag aacaagtact aaattataat
     6061 aaatatggtg gatattcttc acttgaccta tatcattcta atatggatat aaaaagggta
     6121 gtagatgatt taataaatgg atttattcct aatcttggcg aagaaggaag aagtatttat
     6181 aattcactca ctacttacaa tgatgagtat tttgtattga gagattttga aaattatggt
     6241 caagctcaag cagatataaa caggttatat agagataaag aaaaatggaa taaaatgtct
     6301 ttagttaata tagctaattc tggatttttt tcttctgata gaacaatttc tgagtatgca
     6361 aaagacattt ggtttaaaag ggtgtgatta aatgcaaagt gtaatagaat ataactcttg
     6421 ggataaaaat tttaaggctc cgtttggagc tttaaaattt gatgaggaat tgaccatttg
     6481 tgttaaagta aatgagggat acaatatcaa gtctatttcc ctagaaataa atagagaaga
     6541 agaaatgaga acaataactt taaatgaaga attagataat gataaacttg gaaagtgctt
     6601 ttgtggtaag attgaaaagt ttgatggtac aggtgtttat ttttattact ttaaagttga
     6661 tgttgaaatg gatggtcaga taaagacttt attttatgga aaaaacagag ataatggata
     6721 ttcttgtgaa tataattata gtgatattaa taaatatcaa atcactgttt acaaagattt
     6781 taaagtacca acatggtaca aagaaggaat actttatcat atatttgtag atagattcaa
     6841 taatggaaat cgtagtggta aggttgataa tcctaaaaaa aatagtttca tatatggaaa
     6901 ttgggaagat acacctatgt acataaagga tagtcaaggg gatgttataa gatgggattt
     6961 ccatggtgga aatttaagag ggataatcaa taaacttgga tatttaaaaa aattaggtgt
     7021 aagtatacta tatctaagcc ctatatttga agcctctagt aatcataagt atgatacagg
     7081 tgactataaa aaaattgacc caatgtttgg ggatgaagat acatttaaag aacttattga
     7141 taaagcaaaa gaaaaaggta tatcaatagt attagatgga gtttttagtc atactggagc
     7201 agatagtaaa tattttaata tgtatggaaa ctataatagt ttaggggcat atcaatctaa
     7261 agaatctcca tattattcat ggtatatgtt tgaagaattt cctcaaaaat ataaaagttg
     7321 gtgggatgtt aagacattgc caaatataaa tgaattagaa catagctata tggattatat
     7381 aatatatgac aatgatagcg ttataaataa atgggtaaat atgggtatta agggctggag
     7441 actagatgtt gcagacgaat taccaactaa gtttataaga gagttaaaaa aggaattaaa
     7501 aaaggcagat gatgactcaa ttcttatagg tgaagtatgg gaagatgcat caaataagat
     7561 tagctatggt cagagaagaa gttatttatt aggtgaagaa ttagattctg ttatgggtta
     7621 tccttttaga aataacatgt tttctttctt aaaaggagaa attaattcat atgaactttg
     7681 taatagatat atgcagatta aagaaaatta tccaaaagaa tcatttaggt ctaatttaaa
     7741 tttaattgga acacatgatg ttacaagagc taaaacggaa ttaaatgatg atgtagattt
     7801 agtgaagctt gctgtagcta tacagatgac atttgaagga gtaccatata tatattatgg
     7861 agatgaagca ggtctttgtg gaggtgttga cccagataat agaagaactt atccatggaa
     7921 aaatgaagac gaagatatgc ttaattttta tagagatgtt ataagaatta gaaataagaa
     7981 taaactttta agttctggaa atacagaatt tatatatact aataatgatt ctgtattttc
     8041 ttttattaga gttaatgaaa ataatgatag aatgttaatt ttagtaaata gaagtgaaaa
     8101 tgtggaaagt atatcattat gtatagagag tagttttatt gaggaaatac ctataaagta
     8161 tagtcttaaa aatgcaaata gtactataca aattgaaaat aatgagttga aagtagattt
     8221 agactctaaa tcatttagaa tttttagagt aaattaaata tcttttttta gaaaaataaa
     8281 tagaaaataa ggtgaatgtg gaaagagttt tgttttatca aaactttttc tacttttttt
     8341 gtataaatta tcataagaat tttagtattt caaaatatta caaagaagta taaaaaatat
     8401 cataattgtg atattatata aattagtaag ataaaactga actctctacc aaagttgtag
     8461 ggagatttaa cttttataat cttaatatgg aggaacgtct tatgaaggta gatccactta
     8521 caactcaagt atatgactat atatcaaaga aaatccaaaa tggagaatat gaagctaatc
     8581 agagaattac tgaatctgag atttgtaaat ctatcggagt tagtagaaca ccagcgagag
     8641 aagcattaac taggttagca ggtgaaaatt tactagaaaa aatacctaat aagggatttg
     8701 tagtaaaaga atttcaggaa aaggagaaat tagacaccta ttccgtaatt ggagtattgg
     8761 atgcgttagc cggttcttca gcattacaaa atttaactga atcagaccta gtaaaaatgg
     8821 aagaattaac agaaatgatg gcggtctcta taaagtataa aaattacaat agctatttaa
     8881 aattaagtaa tgaatttcac gatatataca taactaaatc agataatcag gtgttagtaa
     8941 atctattgaa ctctttaaga tataatttta tgtcaaagtc atatactagt gataatgaag
     9001 atgagttata taaaatgttg acatacagca ataatcaaca tattgaagta gtaaaatcta
     9061 tgagggagaa tgatttagaa aatgttgaaa gaattttgag agaacattgg aaaacgatac
     9121 ctatggaaga gatgaaaaaa gagaagcttt tataaagttt ctctttattt tttataccat
     9181 tttatcacta tttttttgaa ttattaagta gaaaataata attaatttaa acttgaggag
     9241 gaaaactaat atgtatccaa ataaagaaat tgcaatagaa gagttagaag tggctggcaa
     9301 aatgaatccc ggtccatgga taaaacattc ttacaatgtt gctaatgtag ctaaattgat
     9361 tgcagagcaa tgtgaaaatt tgaatagtga aaaggccttt gtatgtggat tattacatga
     9421 cattggaaga agaactggta tttcttctgt aaggcatatt attgatggat atgattatgt
     9481 tatttctaag ggatgggatg aagtggcgag ggtatgtttg acacattctt ttcctgttaa
     9541 ggatatagaa gctgatattg gaaataaaga tattagtaaa gaacagtatg attttatcaa
     9601 tcactattta aacaaccttg aatataacga ttatgataag ttgattattc tttgtgatgc
     9661 attagcagat gcaaatgggt tttgtatttt agaaaaaaga tttgttgata caacacgaag
     9721 atatggaata tatccatttt cagttgaacg atggaataag acttatgagt ataaagaata
     9781 tttcgagaaa ataatcaaca agtctatata tacattgtta cccaatattg aaaaatctat
     9841 atattattaa atcctaagtt aataatttaa tatccagtat caccagcagt tggtcattat
     9901 gattgactgc ttttattatg cttatattaa aaaaatacac tatggtttgt gtatatcaaa
     9961 gttatatgga atgaaaagag aatttatttt atcaattaaa aaatttaata aattttatat
    10021 taaaattaaa atacaaaaat aggaaaatta tttagtccaa ctaaaccaag aattttgata
    10081 tgtgaaataa gaataatata aaagtttagt aataaaatac ttataaatta aaaatagtaa
    10141 actcaaaagc ttactaaaac tcaaaaaagt aaaaaaaaca aaataataaa aaataagaaa
    10201 gtttataaat actaagtttt aaaaaaatta atataaacaa caaaaaaata aaaagtttat
    10261 aaatattgat atagctaatg tatatgtttt tgagtaactt caaaaaaaca cttgtaaaaa
    10321 tgctgagatg ttaatataat aaatatcatg agtttaataa aagtaaaaat aaaaaataga
    10381 aataataaat aataaatatt aaaaatattt gaatatagag aggagatgaa tttgaaatca
    10441 tggatagcga gtttgcaaat gcagttgaat taagctatga gtgtgctcca ttattagagt
    10501 cgttaaagga atactcagag aaagatatag cttgttttga tgtgcctgga catgtaaaaa
    10561 atagaggtgt agctatatta aataaatatc taggtgaaag tctcatgaaa atggatataa
    10621 attcatcacc aactatggat aatgtatctg cgccaaatgg gattataaaa aatgcacaag
    10681 atttgctagc acaagcatat atggctgatg aggcattctt tataacgaat ggcacaactc
    10741 aagcaataca tgcaatgatt ttgagtgtta taaaaccagg tgaaaaagta ttacttccaa
    10801 gaaatataca taaatcagta ataaatgcgt taatactttg tggaggaata ccaatattta
    10861 tacaaccaga atttgatgaa aaactaggta taagtttaaa tattactctt gaaaaagtta
    10921 gaacagagat agaaaaagat tgtaatataa aagcattatt tttccttaat ccaacttact
    10981 atggagtttg tgcagacctt gagagtatta tagaactatg ccataaaaat aatgttttag
    11041 tgttagttga tgaagctcat ggtgcacatt ttccatttca tttagattta cctccttctg
    11101 caataagttt gggagctgat atggtagctg taagtataca taaaactgga ggagcattga
    11161 cacaatcttc agcactctta ttgaatagag ataatgtaag ttttgaaaag gttcttcaat
    11221 caatcaatat gctacaatca acatcagcat cttatttgct tatggcaagt atagatggtg
    11281 ctagagttaa tctagttgag aatggtgaaa agcaactgtc aaaagcatta aatctatcta
    11341 ggtatgcaaa atctaaatta aacaaaattg atggaataaa agttttatct acagaaatat
    11401 tgaaacaaaa aggtgttaaa tttatagatg aaacaaaatt gtgtataaat gtaaaagaat
    11461 taaatttgac tggatttgaa gtatacgatt tattatataa aaatttttca attcaagtag
    11521 aattgggaga ttcatataat atactggcac ttgtgtccat tggaacaaat aaatcagata
    11581 tagatagatt agtaaaagca ctttctataa ttgcaaagat ttatagaaaa gaatcaacat
    11641 taaatgaatt taatatggtt caaataaatc caattattaa acttaatcca agagaagctt
    11701 tttatgcacc aaaggaaagt gtagaaatca attcatgtat agatagaatt tgtggagaat
    11761 caataatggc atatccacca ggtataccga ttattgctcc aggagaattg ataacagaag
    11821 aaattatgga gtatattatt ttcttaaaaa atagcaatgc atatcttaca gatgtacaag
    11881 ataaaaactt agatagaata ttagtaataa aataaattac tttattttgc aagggcaagt
    11941 atttgttaag tagagtttat caaatagaac ttattaaaag tataaaggag ttggggttga
    12001 aatgaaattt gaaacactag gaagacatat actagtagaa tactataatt gtgatgaaaa
    12061 cgtacttaaa gacccacgtc ttatagaaaa ttttatgaat gattcagcat taaatgcaaa
    12121 agcgacaatt gtggattctg tattccacca ttttaatcct tggggggttt caggagctgt
    12181 tataattgct gaatctcatt tgacaataca tacatggcct gaatatggct atgcagcagc
    12241 agattttttt acttgtggtg atatagaccc atggaaaagt tttgaattat tagagcaact
    12301 tttaaagtct gaaagaagcg agtctacaga aattccaaga ggcttaacta caaaaattaa
    12361 aaaatattca aaaaaagatt taggtaagat tactcataaa ccagaagcag tataaaacat
    12421 acaagagaaa gtgaggatta ttatatggaa ctttggtata cagaagaatg gacagaaaat
    12481 gtaagatttt ctataaaagt aaataaacat ctatttgaag gaaagtctca atttcaaagg
    12541 atagatgtgt ttgatagtga tgaatttgga aaatttctaa caatagatgg attaatgatg
    12601 gtaacatata aagatgaatt tatataccat gagatgataa ctcatgttcc tatggctaca
    12661 aatttaaaca taaagaaagt attagtcata ggtggaggtg atggaggtac agtaagagaa
    12721 ttaagccgtt atcctcaaat agaaaagatt gacatggttg aaatagataa aatggttgta
    12781 gatgtatcta aggagtatat ggatatatgc tcatgtaaat tagatgataa aagagtgagt
    12841 ttatattttg aagatggtgt taattttgtt aagtgtgcac atgataaatc atatgacctg
    12901 ataatagtag attcaactga cccaatagga ccaggtgaag ggcttttctc aactgatttt
    12961 tataaggatt gttatagaat actgactgat gatggaattt tagtaaatca aagtgaaagt
    13021 ccgtattttg actttaatgc taaagagatg aaaagagcta ataaaaaatt aaaacagata
    13081 tttccaatat cagaagttta tcaagcacat ataccaacat atccatctgg tcactggttg
    13141 tttggatttg cttcaaaaaa acttaatcca gtaaaaaatc aagatagaaa tggatgggag
    13201 aaattaagtt taaaaactaa atattataat agtgatatac atttaggttc gtttatgtta
    13261 cctcaatacg tgaaggagat gctagatgaa gaataatttc tatcatatga acacttttat
    13321 gagtatggac aaaaattatg aagaatctaa tcttatagta tttggtgttg gatttgatgg
    13381 aactacttct aacagacctg gggctagatt tgcaagtagc tctatgagaa aagaatttta
    13441 cggtcttgag acatatagtc cttttttaga tttggattta gaggattata atatatgtga
    13501 ttatggagat ttagaaatta gtgttggaag tacagaacaa gtcttaaaag aaatctatca
    13561 agagacatat aagattgtta gagattcaaa ggtacccttt atgattggag gagagcattt
    13621 agttacatta ccagccttta aagcagtaca tgaaaagtac aatgatatat atgtaattca
    13681 ttttgatgcc catactgatt tgagggaaga atataataat agtaaaaatt ctcatgcaac
    13741 agtaattaaa agaatatggg atattgtagg tgataataaa atatttcaat ttggtataag
    13801 gtctgggaca aaagaagaat ttaaatttgc tacagaagaa aaacacacat acatggaaat
    13861 aggaggaata gatacgtttg aaaatatagt taatatgcta aatggaaaga atatttatct
    13921 aactatagat ttagatgtat tggatgcatc tgtgtttcca ggaacaggta caccagaacc
    13981 tggtggtgta aattatagag agtttcaaga gatttttaag attataaaaa actctaatat
    14041 aaatatagtt ggttgtgaca ttgtagagtt aagccctgat tacgatacaa caggtgtatc
    14101 gacagttata gcttgtaaaa tcttaagaga gttatgctta ataatatctg ataa
//
LOCUS       DAYAOX010000096        14146 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.096, whole genome shotgun sequence.
ACCESSION   DAYAOX010000096 DAYAOX010000000
VERSION     DAYAOX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.096"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            402..1805
                     /locus_tag="ACGI57_002166"
     CDS             402..1805
                     /locus_tag="ACGI57_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9717242.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2282..3304)
                     /locus_tag="ACGI57_002167"
     CDS             complement(2282..3304)
                     /locus_tag="ACGI57_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9717243.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3339..4937)
                     /locus_tag="ACGI57_002168"
     CDS             complement(3339..4937)
                     /locus_tag="ACGI57_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HFG9717244.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4979..6244)
                     /locus_tag="ACGI57_002169"
     CDS             complement(4979..6244)
                     /locus_tag="ACGI57_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9717245.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAE
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6386..7813)
                     /locus_tag="ACGI57_002170"
     CDS             complement(6386..7813)
                     /locus_tag="ACGI57_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HFG9717246.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLFASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQIYMGVEE"
     gene            complement(7851..8858)
                     /locus_tag="ACGI57_002171"
     CDS             complement(7851..8858)
                     /locus_tag="ACGI57_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFG9717247.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(9055..10377)
                     /locus_tag="ACGI57_002172"
     CDS             complement(9055..10377)
                     /locus_tag="ACGI57_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717248.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10735..12375)
                     /locus_tag="ACGI57_002173"
     CDS             complement(10735..12375)
                     /locus_tag="ACGI57_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9717249.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12531..13187)
                     /locus_tag="ACGI57_002174"
     CDS             complement(12531..13187)
                     /locus_tag="ACGI57_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717250.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(13213..13914)
                     /locus_tag="ACGI57_002175"
     CDS             complement(13213..13914)
                     /locus_tag="ACGI57_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HFG9717251.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     KKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
BASE COUNT         4807 a         2661 c         1672 g         5006 t
ORIGIN      
        1 aacaaatcct ataattatat tctaatatac tacctactac tttgcgctac aacaatttct
       61 ctatttatat gatttgtaaa atatagtaca acatttttta tcattttcat ttcaactaaa
      121 tttgtaatat tttattcaat acttaataaa tacttaatca atacatgctt tataatactt
      181 tactcaatat ataatcaata cataatttat aatttgtaat actttattca atacataagt
      241 agaaatctct atatacataa attcattttt tcatacatct tacaaattta tttttattta
      301 gataacatgt atattttttc catgaaaaat aaataataaa aaatagtaaa tatggaaaat
      361 ataaaattga ttaacaataa ttaattacct gaggtgtgaa tatgaaaatg ctaaacaact
      421 tttttaagaa aatcaacaat ccagctcctg tctttgcaca gacaaaaatg gaaagagata
      481 atcaaacaga tgattcactt tctacaaaac caaagactac atttagagac gttgctggat
      541 tagacgaagt aaaagaagaa ctatttgaaa tagtcgattt tatgaagtca ccacaaaaat
      601 atcaaaaaat gggtgcaaaa atacctaaag gagtattatt ttatggtcct ccaggaacag
      661 gtaaaaccct acttgcatct gctgtagctg gtgaaacaaa ctcatcattt ttcaatgtta
      721 ctggttcaga gttcgttgaa aaatatgtag gtgtgggagc aaaaagagtt agaactctat
      781 ttgaaaaagc tagaaaagaa gctccaagta taatatttat agacgaaata gatgctgtag
      841 gagcaaagag acatcttgaa agtaacaacg aaaaagacca aacactaaac caacttttag
      901 ttgaaatgga tggatttaat aaagattcca atgtattaat aataggtgct accaatagac
      961 ttgacttatt agatgaagct ttacttagac caggaagatt tgacagacac attcatatag
     1021 gtgcaccaaa ctatcacact agatttgaaa ttctaaaggt acatacagat gacaaaccta
     1081 tagacaaatc tgtaaatcta gaacttttag ctaaaaaaac tcatggtttc aatggggctc
     1141 atctttcaaa tatagcaaat gaagcagcta tatttgcagt tagagatgac agtgagtgta
     1201 ttacatcaga acattttgac aaggcacttg aaagagttat tgcagggcta gaatctaaaa
     1261 actctgcatt ggttgaaaaa gagaaaaaaa tagtagcata tcatgaagct ggacatgctc
     1321 ttgtaagtga tatagtagga atttgtccta tacaaaaaat atctatagtt cctagaggac
     1381 aagcattagg ttatgttctt caattacctg atgaagatag atatatttat actaaggatg
     1441 agttgattgg aaaaataaaa atattacttg ctggaaaggc ttcagaagaa ttaatattta
     1501 atcataaatc tactggtgca aaggacgact taaagaaagt cactgaaata gctaaccaaa
     1561 tggtttgtga atatggtatg agtaacttag gatttatgac tattgatgga aatgataaga
     1621 ctttcttatg tgataaggtt caaaaggaag caaatcgtat agttgaaatt tgttataaag
     1681 agactttaga gatgttaaag gataatcttg aagatttaca ttctgtttct aaattcttat
     1741 ttgaaaaaga aactatgact catgaagagt taaaagattt gataggaaaa gaagctgtaa
     1801 actaatatag ttttaaaaat ttgaggctgt ctcaaaatag agataagcct ctttttattg
     1861 ttaaagttat aaaaatacat ttgattttat acaaaaaagt gcctcataaa ctaaaaattt
     1921 cattttgaga cacttacata ttttaattac atattttaat tttgtctatt tacagaatat
     1981 tatatctaca taaaactaaa ctgctgtaaa cttaactacc tccagtaatg tttgaagtgg
     2041 tataaaagct agtagcctag tttctacact tataatttat attttacata actaaattta
     2101 gtagattttt atactcacaa tttagaacga ttgaaaactt aaaaatttct tatcagatat
     2161 ctacaactta attaggtatc tatcacttga ttaaatatct atcacttaat taggtatcta
     2221 tcacttgatt aaatatctgc catttaatta aatatctacc acttaattaa atatctacca
     2281 cttaaagtgt agaacctctt tcaatcaatt caggttttaa catttcaacg actgactctg
     2341 gattttcttc atttaataag gacttaagta tgttaattgt tttttcaatc aacaaatcta
     2401 gtggctgtct aatcgtcgtt aaattataac tcttccatga agccatttga atatcatcat
     2461 atcctatcac tgataaatcc tctggcaccc tcaacttgta ctcaaacctt gcagcatcca
     2521 ttacaccaat agctatcaag tcacttgtgc aaaaaattgc tgttggagga tttgataatg
     2581 ctaacattct cttacctgct tcatatccag aatcataatc atagtttgca gattcttctt
     2641 taaggttatg aatcccactt tgtcttaact tagagtaaaa tccaattttc ttttccattt
     2701 cttcacttgt ttctctcgta aattgaatat atcctatatt cttatgacct ttcttaagca
     2761 gatattcagc agccattccc ccaccttcaa ttgggtctac atagactgta cttattttta
     2821 taccaggtat aaaacgatta aaaagaatca caggaatatt gttttcttca caagtctcag
     2881 ccatcacctt attcatggcc gaagctgtaa taatcacagc ttctacttga aactgaatta
     2941 cttttgaaat tatactatct atactttgtt gtctaggaac tttaaaaacg agacactgtt
     3001 tacctatctc ttgaattttt tctacaaata aattgataat tctattgtaa aatggaccta
     3061 gactatcacc aactatcaat cctataacat tagtttttcc actaatcatt ccccgtgcta
     3121 ccaaattagg aacatatcct atttcatttg ctacacgaaa aattctttct cttacttcag
     3181 gttttacgcc ctttccattt gcactaccaa atgctcttga aacagatgat tgagatactc
     3241 ctgccaatct tgcaacatct tttgaggtag ccgatttatt ataatgaact ttcttttgat
     3301 tcatttagta tttctcctta aatatctagt atttcttctt aaaattgtat caaaaataat
     3361 ataatatatt ggataataaa aaaagctaat acaattaatt cttgtttacc taaagaaaat
     3421 ggcactatag atactccatc tactttatcc attccttgag aaataaggtg atttctcata
     3481 ccaaatgcag tgtatttttt aatatcatct gcattatgat ttttctttaa aatgacacta
     3541 aaaatatatc caaatatcaa tgttattgta gcacctattg ctggattcca caaccaactc
     3601 acattatatt tattagaaat aaaaaatcct aaaactaacc ctacaataaa accaatagat
     3661 actcctttat cattagcttt atatgtaaac attgctagta caaatgcacc tgtagctggt
     3721 ccagcaaagt atgagatata gttaccaaca aggtcaagaa ttgatttgat gcttcctcca
     3781 aatcccataa taataaaacc tgtcataaca actcctataa ctacagttat aatcattgta
     3841 acttttaatg atgattcttt gttatctttt ttgaaataaa actcataaat atccttcgta
     3901 aaaacagttg tcatactgtt taatatagag tcgatactag acattgctgc tgcaaacaca
     3961 cctgcaataa taattccaat ggctcctact ggaagttcat ttaaaataaa agtaatcatt
     4021 atttcatttg aagtttcaaa tgtccttccc ccataaaaaa caaataaaat tactcctacc
     4081 aatagcataa agtagtaaac taaattcata acaaatgcac taactacaaa agaatttttt
     4141 gcacatttta aagatttaga tgttaaaact ctttgtactt gagattggtc aaaacaaaag
     4201 tatcttatcc acattacagt tccaccaatt aaagtagccc aaaaagtatt tgtcactgaa
     4261 atatccctac tgaaatcaaa tgtatttaat tttccactct gttgtgcaat ttgtaatgta
     4321 tcaaagaaac ttagattcat tccttttaag gctacaataa ttactataaa tacagcactt
     4381 attactacta tcatttgaat actatcagtc catataactg ccttaatccc acctaataga
     4441 gtgtatataa ttgatacaag tacaactatt gggactaaag cataaagtga ccagccagta
     4501 atcatttgaa taataagcac tggaatatat accatagaac tagcttgaat caatgaatta
     4561 ataaaaaatt gaataatagt taatcctctt gaataatttc ctaaacgaag tcccatatat
     4621 tcatatatag aggttatttt taatttatag ataactgggg tggtaatgct cagtgcaaaa
     4681 aatattgcta aaggtactgt aatattaacc attactggat acattccatc tacatatgcc
     4741 catccaggac cacctataaa gccatttgca ctaatcatag tagctgcaac tgataaagca
     4801 attggaatcc atggcattga cctacctgcc aagaaataat cttcttgatt tttattatct
     4861 tttcctgaat aataaccaat aactaacatt aatagcaaat atcctattat aatacataca
     4921 tcaattgatg aaatcataag ccaccccttt ctaagtaaca aaataaattc ctagtatttt
     4981 attttttatt atttaacgtc tctggtaaga ataaccatac aatagcacct gctacaaaga
     5041 ctgcgattga tatgtaaaag atagtcatca tacctaaagt accagaagct gtaccagcta
     5101 tagcaggtat tgcaaacgta gatataatcc ttcctccatt aaatgacata ccagatgaca
     5161 acgaacgata tttacttggg aacaactctg tgtagtatgc tccccatgct ccagaatatc
     5221 caacagcaac acctagtaat actgatatca tatagaatgt tgttgtatta tctccaccta
     5281 attttgtata tataaaaaca aatactgctg ctaatgccat acctataaaa ggtatttttc
     5341 ttccaaattt atctgcaatt gaaccccaga ataaatatcc tactaattga ccaattcctt
     5401 gtaaactata tatagactca gccactgctg ctgacattcc tattcctgtc actaggtatg
     5461 tagttgcatt atttgaaaat gctgaatatg ctgtaaaatt tgcacctgat aaaagaatcg
     5521 ttcccaatcc aatccataag tattttccct tatacatact tatatagcta atcttttcac
     5581 cagtttcttt tcccttttta actaattcct ttgtatactc caaatcatct ggcacaaaga
     5641 agattgctaa gatagctcca actataggtg gtataatcgc agtatacatc aatatttgcc
     5701 aatttgcatc acctaaaacg ccatatactt tcccaccaaa aatcattgct aatgaaaata
     5761 aagcagttaa tatagcagct aacttaccac gatatttact tggaaacata tctgcaacta
     5821 aaggcatagc tgttccaaat acgcctccca acactactcc tgtacacaaa cgtgctattt
     5881 tccataacat aatatttgta ggagataaca ttggtataaa tgttgtcaat ccaatcagta
     5941 aagcaacgct aattaaagtc ctcttcttcc caatttttgt agaaatcacc ccaaaaataa
     6001 atgtacctgg aattgtacca aaagacataa ctgcaaatat ttcttctgtt tgttttaatg
     6061 aaaaaccaaa tcccccatct gctaaagcat aaatcattgt tggtttaata aaagtaccca
     6121 ccataaaatt gtagcaataa aaaaagtata ttaaagcaat tgaaaaaaat gcaattcctc
     6181 tttttaggtt tgaatagtct tttcctaata tgatatcatt ttctatttca accgttgttt
     6241 tcatattttt ataattcccc cttttaaatt tagtcataat tttgctaaaa gtttaaacat
     6301 ctatttttca tatacattcc ccacttaaat tttataaagc aatacttccc tatatatttc
     6361 aaaactattt atactctaaa cagctttatt cttctactcc catataaatt tgttttaata
     6421 catccatacc atcaaaagtt atccactcct cctttacttt accttgtagt atatgataat
     6481 gattgattcc cattacttct atatgctttc cagaaggctg tccaaaaaat cctatacctt
     6541 catttattcc acgtaatctc catcttacag atacatcata accttcattt ttttctcttt
     6601 gattacaagt tactctttcc acttcataac ttccatttgg aattgatgca aataaactta
     6661 caatcattcc ttgaatctca tcatagccat ttaaatcttt atcacatata aaatgtacta
     6721 ctgcattatc atgatagtat tttttaactt catttatata tttataatta taaatacggc
     6781 ttgtcatttc aagcatcatt tcgccaacag aatcatcttt tgcttgatac ttagttggca
     6841 taaattgacc atccatagat tcacagatac cataagttga ttgtaaacta agcactttag
     6901 attcacttgc ctttgccata ctctttgcta cttcctgagg gtttaatcct aattgagtaa
     6961 caatccataa attatctcta accaaccatt cttcatggat tctattgtta gttgtggcac
     7021 aatcaattac agttctaaag tttatttttc tacctgttgc tggtccaaaa ttactatcac
     7081 ctaagttagt tgctgtagac aaaacacggt gagaagataa aaaaccatca gcaccaaagt
     7141 tagaccatat tacattttga ccaattaatc ttctatcagg aaacgcatgt aatgtttgaa
     7201 gagtattgga aataacatct ttaataccaa ctaaatttga agagccacaa tgcattgtaa
     7261 cattattgtg atatgtatca taaataacac ctataccttt ttcttcccag atattatgtg
     7321 taatcttcat aatataatct acaaaatctc tatacttaga atcaaatcca ggtatttcct
     7381 gttccctttt tgtctgtttt gaaaaatcat tgtaatccat tactccaaca ggcgttactt
     7441 ctaatgaatc tccatagtaa atttttgtag atgacatatt atcttccccc ttaaatataa
     7501 cttttttatt tttttcagta agagtatttg tatttaattt atctgtatcc actgtatcta
     7561 tgttaacttt atttatgtcg gatttatttg tgtttgtttt atctatattc cctttatcta
     7621 tatttccttt atctatattt cttttatatg tgttcgtttt attctcattt gtttgatttt
     7681 catttatatt actaaaatta gtttgattca ttttagcttt gttttcttta accgtacatg
     7741 attcaagccc acttgtagaa acttcttttt cttcctcttt acttgttgca ttatttttgt
     7801 tggtagatgc cactttatat ccccctttgt tttagaatac gtatgcataa ctaagcatta
     7861 aaaatacttt tagttctctc aagaatatca agcccttgtt gtttcaacca ataaggaata
     7921 tcaattaaca cccagttttc ttgaagttta tcgcccttac gataataaac atcaacaact
     7981 tgcatatctg ctttaacctc tccacctggt aatcctaaaa aacctccaca tggtacatta
     8041 gtcagattag gccatccaaa gaaacaagca aaattacctt ctgcaaatct acatacatgc
     8101 ccattaaaag tctttccttt taaattattt ctaaatggta gctggtgttg ctcttgatat
     8161 ctaggtatag tataagaagc accaattcca cctggaccat accaaatcat atctttagcc
     8221 caagattttt caagtatttc tggtgggcat cccatagcac cactttcatt tagttcagat
     8281 aaatcatcta ccattttatt tactaatgct aaagttttta ctccttcaac ttcatctgca
     8341 tcttcaaaaa gtaatccatt atggtcaatt ggccctggat atacaaaata tgaacctgtt
     8401 tgtggtggca atggattaac tcctgcttga atcataaacc caattatatc tacaaataat
     8461 ccagttttag caattttacc atcttgaaca cagttaaatt ctgcatatct taaactaatc
     8521 attttacgag tatgtttaat gccaagccaa tcgttatcaa aaagtcccat aaaatgcccc
     8581 atactcatta cccattcact attgtcaatc tcatttgttc ctgcaatgaa aatatcttgt
     8641 cgtctttgca tattactcaa tgattttttt aaaggcttcc aaaatacatc tgctacattt
     8701 tcagtgcctt cttgttcacg aaatggatac acacctttcc agttataatc tgatttcata
     8761 tgttgcttta agacattttc aacctcatcc ccagtagcat tttctaatgc ttcaaaataa
     8821 tttcttacaa ctttttttga atcctgatat tttcccataa ttatctcctc ccagatttat
     8881 ttaagttata aattaacttt ctcatttcat gcatacgatt tcctttttaa tgatttttta
     8941 gctgttttaa gtttatttta tgaatacata tgcatttttc taaatattaa catatatcta
     9001 aaaaattgta aagaaaatgt tatcataaaa atttaatttt tgacttatat ctatttaaaa
     9061 caacagaaaa ggtttaaaac gtaatattct actgggtcta ccatacttgc tcataagctg
     9121 ttcactcact acctcacaat aaccagaatc acacaatcta agtataatcc tgtccatagt
     9181 tctaatactt acaccacata tagtagctaa ttcctttgat gtaaattcag tttttccact
     9241 atttattatt ttattgaata ctttatatac agtattagca cttagccctg tctctgtagc
     9301 aatttgataa aaacgttcat caaaactatt tttctctttg acagtaggat ttggctttag
     9361 tggtcccata atttctccat tttcaaaaac tacatatgct gcatcatttt tttgactttg
     9421 agccatctta atactctcat aagcgttaaa tttagcttca tttgctgttt ttccataacc
     9481 aattcctata taagtgttta taactgaaac ttcacgaatc atatccaaca aataaatatc
     9541 tctgtatttc tttgtttcta actcaagtat ttttttagtt gtaaaaatca taaattcact
     9601 tctactctgc tctaccaaaa caccatctat acggctattg aaatggtata gtttatcaag
     9661 aatcttaatc ctttgtgaaa gataagcgta ctcatcttcc ttcattagtg aatattctga
     9721 aggcatgttc agtttaatag caagaacaat aatctgattc tcattgtttt tctttgcaat
     9781 ataacgcatc tgaatgtttt ttatactttg caccattacg tcctcagaag gcaaggctaa
     9841 aactactgga atattatctt ttttcattat atgataggcc tcttcaagac ctgtaataca
     9901 acaacataca tcattatttt gataattata tttatgaaat tcaagcacaa actttatata
     9961 gtcctcttct aaatactttt gctctgcaaa aaacatatgt tcatccttaa attcaactcc
    10021 aatttcttta tacaatctct tcatcatagc cttatcataa gtatctatac taagtctact
    10081 aatatcgaat ttaagcaaat atgtaatttc aagcaacact ctgtacaatg ttgtttcatg
    10141 tctaggaata taatcagaca atacaaaatc tttttgtctt ttttcaaaaa atttaaatgg
    10201 cgacttacct gtaaaaagaa ctgcatccac tctacctcta tatttttcca ataaatcagg
    10261 tacttcaata tatctatcat atctaagaaa atctgcttca atattaggaa aattcttttt
    10321 acttactgct agtattttat cgattaccat acctggtcct acataaagta ttttcatttc
    10381 gtctctcctt tcctaatgat actattatct cttaaaacat tttctaaacc taccatgact
    10441 aaagccacag gactcctgat tcatagaatt ttgaataatc aattatacct aatataaaaa
    10501 gttactttaa gttttttaat tacgactatc tgttgaaaca tttttgtatt taaatttttg
    10561 tatttaaatc ttattcatta atattaatta cattttacaa ttaatatatt gttttttcta
    10621 cttaaagtta aattctttag aaaacacaag tagagtaatc tttattaatt taacaaaaat
    10681 agccttcctt aatattcaaa gaaagactat ttttataaaa gttcttaatg tattctattt
    10741 tgctatgcta agaggatttt taattacacg tccagcacat acatcagttt gctttcctcc
    10801 tgccaatgca attttaccat tcaccaacac atattccact cccactgtat actgtttagg
    10861 attactataa gtcgctttat cttctattgt atctgggtca aataccacaa tgtcagcaaa
    10921 ataattttct ttaagtagac ccctttcttt ataaatgcct aagcgttttg ctgaaagata
    10981 agttatcttt ttaactgctt cctcaaaagt caacatacct ttttctctaa catagtaaga
    11041 gaaaattcgt ccaaaagtac caaatgcacg aggatgaggc tttccaagag aaagtaaacc
    11101 ttctgtagaa aggtccatac tatcagaacc tatacatgac tttggatggc aaacaacctt
    11161 atctgtatcc tcttgacctt gtgtatgaac aactacttga attttacttt tttcttcaat
    11221 caaaagccta aagaacgtat caaatggatt ttcaccattg attttggcta cctccattaa
    11281 agttttaccc tctacatatt tatttgcttc agtcattaca gaggctagta caacaccgtt
    11341 ccacccacct gctaattggt agaaacattg ccatccagga atcccttcct ccaactcttt
    11401 tataattctg tctctatttt cttgtacttc taatcgtttt accatatttt ctacaccgcc
    11461 ttcaaaagcc caaggaggta aaagtgcacc caaagatgtc attccagctg tatatgggta
    11521 acaatcaaaa gttacttcca aaccatctgc ctcagcttca tctagctttt taaatacctc
    11581 atcaatagtt ccaaaattct tatgacccat aacttttaaa tgagaaatat gtagaggaac
    11641 tcctgcagtc ttagctatat aaattgcttc atcaagagct tccatttctt catcaccttc
    11701 atctctaata tgagatatat agtatccatt gtatggtatc aattccttac ataattcagc
    11761 catttcttct ttctttgtgt atgctcctgg taaataaact aatcctgatg aaagagctac
    11821 acatccagct tccatagctt ttctaacttc caacttcata tttttaagtt gctctaaagt
    11881 agctggttcc tttgaaaatc ccatttcatt gatacgtaca ggtccttgtg ctacataagc
    11941 tacaacattg actgctggag aattttcagt taagtaatca aagtattctt gcattgtatt
    12001 ccaatgaagc tctaattttg ttggaattgt accaattatc ctagttccaa gataatcaat
    12061 taactcttgc ttcttctctt ctcttatcgg agcaactcct ataccacagt ttgcaataac
    12121 atctgttgtt acacctgtat ggatacggct tcttgtgtat ttatcataca caagagataa
    12181 atcagaatga gtatggaaat ctataaaacc aggtgataca aattttccag ttgcgtctat
    12241 agtctcttta gcctcttttt gacttaaatc tccaatagcc atgattttgt ctcctacaat
    12301 ccctacatca cttttatatc ttgaatttcc tgtaccatca acgattattc cattgacaat
    12361 tttaacatca aacatgcttt ttctcctttc aagacttatt tatattcata tttcttaata
    12421 aacaatccta ttcttttttc tcaacaaata attctagtct ttctccttaa taaacaatcc
    12481 tattcttttt ctcaacaaat aattctagtc tttctcctta ataagcaatc ctattctttt
    12541 gttttattgt tatcaattac aaagaagacc cttgtgataa gtatcccacc taacacaata
    12601 aacaatgtta ataatccacc tggtatacca attttttcac caaagtacat aattgctaac
    12661 gctacagcaa tcgtacatcc accagctcga atatccttac gtgcaatatc aacataaatt
    12721 gctccaaata atgctggaag tatataatta aatgaattag taacaaattc aggtaacaaa
    12781 ggaattactt tatagcctac aatggtaaaa atggtaatca tagaagctga tacaaagatt
    12841 gaacaacatg taccaattgt tccaacaact tcaccctcat gagttcctgc ttctacacct
    12901 gcaattttct gtgccattga tgctgcaggc atacgtatat ctccaacaga acctgcaagc
    12961 caacctatat aagaacctgg agctcctagt gtaggataat aagctatagg ttgaacaatc
    13021 caactaaccc cataagtcgc agcgactaac ccccaaattt gaaatataac agaaagcatt
    13081 ggcatctgac caaagcgaat acctacatag atagcgggaa taaagtttgc aataattgca
    13141 agacctatag aaattctccc tatttttatc atctttttat catacataat tatcacctct
    13201 tatccgtttt aatcacgata atataatctg tgtaccaaac ataccaatta acatggcaat
    13261 acctaaggat aattcttgta atcgttgatt ctttttaaat aaagttgtca atactagcat
    13321 aactatagct gaaataattg ctggtgtaag ttttggtatt tcaaatgttt ttatttgatt
    13381 tgaaagaagg taagcaaata atccaactgt agcacctgca agtagttttt taacccaagt
    13441 aggattatac ttcatgttca tcttttcaac aattccacgc attctatgac ccaaaataaa
    13501 tacaacaaat agccaaccta agttatttaa agccatcccc catagtgcat acgaaaaagc
    13561 ttctgctcca aatgcagcac tacctacttc taccccagat aaattggatg taagagatat
    13621 tactccaagc tctgttctag ctgctccgac atcacagagc ctcatccaag tcgtaggagc
    13681 gccaatcaca gctaccagtg ataataatgt aataacagga gatagagaag gtccaatggc
    13741 tgtaacacta gctgagcgaa ttgctgcttt aattcctttc ttttcaatac caagatttga
    13801 agcctctttt agtgcctctt tcataaatac gattgcttga acaactgaaa taattactaa
    13861 aaaactagaa acaatccaaa gacctgggct attaatcatt tcttgaaact tcatacttta
    13921 ttcctcctta aaataaattt ttcagcattt tattgtgagc tttctttctg taatcttgtt
    13981 atgtttttat attgttttaa tttattttat ctaagatata gtatctttaa taggagatta
    14041 tctgtcttaa agtctcacta aaaacattaa tacttaaata ttttattcat aattagtaaa
    14101 tatcagaaaa ttcacatatt atttttttaa cctgtaatta acttat
//
LOCUS       DAYAOX010000097        14012 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.097, whole genome shotgun sequence.
ACCESSION   DAYAOX010000097 DAYAOX010000000
VERSION     DAYAOX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.097"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            444..1514
                     /locus_tag="ACGI57_002176"
     CDS             444..1514
                     /locus_tag="ACGI57_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717252.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="ACGI57_002177"
     CDS             1498..2367
                     /locus_tag="ACGI57_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFG9717253.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="ACGI57_002178"
     CDS             2411..3271
                     /locus_tag="ACGI57_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFG9717254.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="ACGI57_002179"
     CDS             3302..4606
                     /locus_tag="ACGI57_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9717255.1"
                     /translation="MKLKRKLATGLAITLLLCSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="ACGI57_002180"
     CDS             4669..5931
                     /locus_tag="ACGI57_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFG9717256.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="ACGI57_002181"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="ACGI57_002181"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFG9717257.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="ACGI57_002182"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="ACGI57_002182"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFG9717258.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="ACGI57_002183"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="ACGI57_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFG9717259.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /gene="hprK"
                     /locus_tag="ACGI57_002184"
     CDS             13008..13937
                     /gene="hprK"
                     /locus_tag="ACGI57_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFG9717260.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
BASE COUNT         5697 a         1615 c         2544 g         4156 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaattttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tactttcaaa ccaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa attacattgt gatagcaaac catgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacttc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt taccatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggcccaagt ggatgtggta aatctactac gttaagaatg attgcaggtc
      601 ttgaagatat aacttcaggt aggttgaaga ttgaagaaaa tgatgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaagtc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcaatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taaaaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tgggaaaaat gatgatgtat
     1261 ttagtctatt tggaaaagtt gtaactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagag ataagagtca aaacggaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aatcaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacaaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatataa tatcttaata gtgttatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccac tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg gcaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataaggt ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatagaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcta catcctttaa aaatccacaa gaaatgatgc aattaccgcc
     2581 cactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttgctaa acagtgtatt tgtaacagga agtgttttaa ttgtacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gctatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat gttccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aattattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat tatgttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataaa gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattatgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacaccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta agttctggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcttacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaattgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtactca
     4921 aaggacataa aagcaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagttc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatatcg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacaca attatggctt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggctag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gagttaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatggtaat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 aatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataattg tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag ggccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatatc
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaaggacaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aagaaggatt tgtaagagtt
     8641 agagttaatg gtgaaaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaagaat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagtaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgc
    10021 ataacaggtg tttcaggttc tggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gttttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc acaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaacat tgttttcaaa tcaattatag aagggaatat gggctttaac
    11101 ttgtttaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttgggccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggggta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaagcaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagatgcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcggg aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacatac aacaattcac ggtgtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga actgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactagg actggataaa gaatatgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tggactacac atcatttaat taaatattga
    13981 ttaggattat gtcctacacc ttataaatta aa
//
LOCUS       DAYAOX010000098        13992 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.098, whole genome shotgun sequence.
ACCESSION   DAYAOX010000098 DAYAOX010000000
VERSION     DAYAOX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.098"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(200..1042)
                     /locus_tag="ACGI57_002185"
     CDS             complement(200..1042)
                     /locus_tag="ACGI57_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9717261.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1294..3078)
                     /gene="pepF"
                     /locus_tag="ACGI57_002186"
     CDS             complement(1294..3078)
                     /gene="pepF"
                     /locus_tag="ACGI57_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFG9717262.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3144..3809)
                     /locus_tag="ACGI57_002187"
     CDS             complement(3144..3809)
                     /locus_tag="ACGI57_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFG9717263.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(3960..4121)
                     /locus_tag="ACGI57_002188"
     CDS             complement(3960..4121)
                     /locus_tag="ACGI57_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HFG9717264.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4199..4708)
                     /locus_tag="ACGI57_002189"
     CDS             complement(4199..4708)
                     /locus_tag="ACGI57_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFG9717265.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            4914..5132
                     /locus_tag="ACGI57_002190"
     CDS             4914..5132
                     /locus_tag="ACGI57_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9717266.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5150..7096
                     /gene="feoB"
                     /locus_tag="ACGI57_002191"
     CDS             5150..7096
                     /gene="feoB"
                     /locus_tag="ACGI57_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFG9717267.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(7275..8330)
                     /locus_tag="ACGI57_002192"
     CDS             complement(7275..8330)
                     /locus_tag="ACGI57_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9717268.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8378..9178)
                     /locus_tag="ACGI57_002193"
     CDS             complement(8378..9178)
                     /locus_tag="ACGI57_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9717269.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9171..9962)
                     /locus_tag="ACGI57_002194"
     CDS             complement(9171..9962)
                     /locus_tag="ACGI57_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9717270.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(10002..10424)
                     /locus_tag="ACGI57_002195"
     CDS             complement(10002..10424)
                     /locus_tag="ACGI57_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9717271.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(10440..10919)
                     /locus_tag="ACGI57_002196"
     CDS             complement(10440..10919)
                     /locus_tag="ACGI57_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFG9717272.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(11051..13807)
                     /locus_tag="ACGI57_002197"
     CDS             complement(11051..13807)
                     /locus_tag="ACGI57_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9717273.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDIIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4515 a         2426 c         1600 g         5451 t
ORIGIN      
        1 ttctttttta ttctaacatt gtaatattat tttgtcaata tttccatatt cttagagata
       61 agatactaaa atacctattt tatctaaaga tatttatatt tctacattca ataatagaca
      121 ataccctgca agttaaagca tcatttttat gagcttatct cacagggtat tatatgttta
      181 ctttatatta taactcttgt tattgattaa ctttaataaa tcctttttct gaaacaagtg
      241 cttgtccttc atcacttaat atataatcta ttaaagcttt acctttttct gacaaaccat
      301 cttctttatt aactaatata aatggtcttg atattggata tttattagct attacatttg
      361 catcagttgc ttcaacatca tttattttta aactatttac tttctcatca acatatccaa
      421 atgatatata tcctatagca ttttcatttc cttcaactgt agtttttata tttcctgaac
      481 catcacttat ttgtgcatct ttagttagtt cttctgaatc aaaccctaca atttcttgga
      541 atccatctct agttcctgaa ccttcttctc ttgatactat tactattggt gcatcatttc
      601 ctcctacttc tttccagtta gtaatctttc ctgtatatat acctttaact tggtccattg
      661 tcagattttt tactgagttt gcagtattag ttatcaatgc tataccatct attgctattt
      721 tagtttcctt taatccagca cttttttctt catcttttaa atctcttgaa ctcattccta
      781 tttcagatgt tccttctata gcatttttta tacctccaga agaaccaatc tgttgtactt
      841 ctacactaac accttgattt ttttcttgaa acttttctcc gatagtttcc ataagaggac
      901 ctattgaagt tgaacctgaa acagaaactt tgcttgaatc tcctgaatca cctgaacctc
      961 ctgagttaga actacatcct actgctaaag ctccaactaa cattgtacta gctaaaagaa
     1021 cgcctatttt ctttttaaac attttactta cccccgctta tattttaatt ttttgttgtc
     1081 atttttctat attttaataa tacagattct ttgttaaatg aatattagag cagggttaag
     1141 taaatgtaaa tattgttaaa tacatgtaaa aacttaattt tatatgattt attcttaatt
     1201 agattaactt tttaaagtta ctttaggcag atataaacat aatacaactc atattatcag
     1261 aagtcttcat tgaagctatc aacctatcac atcctataat tctttttcta gtttattaac
     1321 taattcagca aatacattta aggcttcctc tatagattct tttttcgtca tatctacacc
     1381 agcagatttt aattgttcta gtgggtattc tgaccctcca ctttttaaga agtttatata
     1441 tctatctact gcagtacttc cttctgaaag tatctgttga cttaaagcac ttgcagcaga
     1501 aaaccctgtg gcatacttat atacatagaa gtttgaataa aaatgaggta ttctcgccca
     1561 ttctattcct atttgtttat ctactattgc agattttccg taatactttt catttaattt
     1621 gtagtatata ttagtaaatt catcagcagt aagtggttct ccactctcta ccctttgatg
     1681 tacaattttt tcaaattcag caaacatagt ttgtctatga actgtagttc taaattgttc
     1741 taaataataa tttaataagt atatagtttc atccttagac ttagaatttt ctaataaata
     1801 atttataaga agtaattcat ttaatgtaga tgcaacttct gcaacgaata tcttatagtc
     1861 cgaatataaa aatggttgac ttgttcttga ataatgactg tgaacagagt gccctaattc
     1921 atgaattaaa gtgaatagag aattaaggtc atttttatag ctcattaata tataaggatg
     1981 tgaatcataa cttccccaag aataagctcc tcctttttta ccttcattat catatacatc
     2041 aatccaccct tcatcaaaag cactttttat gaccttcaag tattcttctc ctaaaggttt
     2101 aagtgcctct aatatgatat cctgtgcttt atcatatggt atatccatgt caaatttatc
     2161 tgtcaaagga acatacaaat catacatgtg tatttcattc aatcctaaaa atttcttttt
     2221 aagctcaaca tatttattta gattaggaat acttttatct atagctgata taagattatt
     2281 gtaaacatct aaacttacat catctgaaaa taaagacgct tctattgctg aattatactt
     2341 tctagttttt gcgtaaaata tctctgattt tattccacca taaagcgttg aggcaaatgt
     2401 atttttatat ttctcatatg ttgaaaattc accttcaaaa gcgtcttttc taactctact
     2461 attcttactt tttataaata aagagtagtt tgaatgagaa agcttaacct tattcccctc
     2521 ctcatcttct atctcaggaa attccatatc tgcatatgct aacatatcgt atacattttc
     2581 tggaattgtc gataattctg atgtagctgc aagtatttct tcctctcgtt cactcaaagt
     2641 atgtggttta tctctcaata tatcctttat atattttgca taaaaggata gcttctcatc
     2701 ttttaagtat tcacttaact tattctcttc tatcgccagt atttctggaa ctatataaga
     2761 agtagccaca cctaaatcag tagataacat ttctgtctta gtggctcttc cttgattatc
     2821 attaattcta gtatcttcat gttgtttcat atgagtatat acatataaat tttgaacaat
     2881 cctagatgca ttttcagaga tatttaatac tttatataag ttctccttac tctcagaaag
     2941 cttcccttta tattctttta cttcttggat aagatttttt actttactta tgtctttttc
     3001 aatttcctct ttactggagt acatcttatc tatcttccat ttgtactttt cttctattgt
     3061 acttctatca gttcccatat catacacctc tattttaatt tgttttatat tttattcact
     3121 ttacaaaata tattgtatat attttagtcc aaataatcta catttattac tccttctatc
     3181 atatttattt ctttcataac gtcgtgtaag tgtattctct tagcataaga tacaaaaaaa
     3241 ttaaccttcc agtattcttc attacgacat tctatttcca tatttcttac gataagttga
     3301 tttttttcaa atttatcata taaaaaaggt atagaatcca aattgcttaa ttttaatatc
     3361 aatacattct tttcttggct aaataaaaac gtattgatta tcctaagcac aaataatgct
     3421 atcattacaa ataatccacc tagaattgcc aattcataat aaccatatcc aactgctata
     3481 ccaatacatg cagttgccca catcccagca gctgtagtaa gcccttttac tccaccttta
     3541 ttttttaaaa ttgctcctgc acctaaaaaa ccaattccag ataatacttg agcaggcatt
     3601 cttgctgggt cagcatttgt atacaaatta taatcaaaga ataattgaat cgaagttatt
     3661 gaaacaatac aagaacctat tgcaattagt atatgtgtcc taaatcctgc aaactgtctt
     3721 attttttctc tctctaagcc tattattcct cctattatta gtgctaatag taaccttatt
     3781 gctatctcct tacttcctat atcaatcata atataaaacc ctcctgactt aattacaact
     3841 tatgtcctac agataattta cacatcagaa atactatttt atatcttaat attattatta
     3901 atatatgtaa aatatagcca tctgttaatt tttgatacaa aaaaacccac aactgtgggc
     3961 tatttgctta ggtacatgta tttatttatt aatatatcta ttttcttact tgattctact
     4021 aactcttctt gaaatatttc tggatattct atgtatgtat ttatctcttc cctaactgct
     4081 tcaatctcat tttttaacgc ttctaattca ttgttcgcca taataatcac cactattaca
     4141 aatttgtata tttttatata cattttatca aacaatatta tattttgtca atattatgtc
     4201 aataaaactt actataaaaa tgctttaatt taggtattgt atacctggat actaagccta
     4261 caaacatacc agttactaat gatgccatta acataaatgg taagtaccct atagcaagta
     4321 tattttctat gattaaactt gcaactatta attgacctat attatgccct attgcaccta
     4381 taatgcttat ccctattagt gataaatctt ttattttact cgctaaatac ataaataaaa
     4441 gactcagata agctcctcct atactaaata gtagatatga taaagggcct gcaaatattg
     4501 atgacaaaat taccctgata gtaacaatta taacagtatc tttgacacct aacaacatta
     4561 atgaaattaa agttataata ttgctcaaac ccaattttgc ccctggaaat ataactataa
     4621 gaggatttgg tatatatgtc tctaatacat ataaagctaa actataccct atcataagac
     4681 ctaaaaatgt catttttcta gttttcatta agttaactcc tccaaatttt atctatcatc
     4741 ttacattata atacaaaata tataaaaaca cctaatttag actaaaatat ttttaatcta
     4801 tttagaataa atattagtta tttcaatgtg caattacctc tataaatctt gactttttgt
     4861 atcatacttg ttacaatttt aatgataatg aatttcatta ggaggtgtta ataatgactg
     4921 tttacgattt aaaaatagga gaaaaaggat ttattaatag tatagatgga gatattaaat
     4981 tatctaaaag attgcttgca cttggatgta taaatagtac tgaaatagaa gttaaaaagg
     5041 ttgccccact tggagaccca attataatta gatttagagg ctttgacctt gcgattagaa
     5101 aatctgatgc taaaaatatt tctttacaat aatagaggag gagctacccg tgattaatgt
     5161 agcattatta ggaaacccaa atgtcggtaa aactactgtt tttaatctac ttacaggttc
     5221 aaatcaatat gtaggtaact ggcctggtgt tactatagaa aaaaaagaag gatttcttgg
     5281 aaaagagata aaggttgtcg atttacctgg tatatatgca atggatactt tctcaaatga
     5341 agaaaaggtt tctaaatcat atttagaaaa tgaagatgtt gatgtaatag taaatgtagt
     5401 tgatgcttct aatttatcaa gaaaccttta cttaacaact caacttatgc aatttaacaa
     5461 gcctatagtt atattgctta atatgttaga cattgcagaa tctaaaggag ttaatataga
     5521 tgccaaaaaa ttaagtgaag aacttggagt aattgttgtt ccaattatag caaaaaagaa
     5581 aaatggtgtt gataggatag aagaagagat taagggagct acgcaaaaag cttttatata
     5641 tcagaaaaac tttggaagtg aaacagaaac atataaaaaa ttagaatcca ttttatcgag
     5701 atgtacaaaa actacatata cacaaaagaa atctatcagt gataagatag acaatatagt
     5761 cttgaatcct atattagcat atccgatatt tattggtgta ttgttcctct tatttaaatt
     5821 tacctttgac tgggtcggag gaccactaca agagggattt gctagtctta tagaggctta
     5881 tatttctgca cctgttagtg atatgttatc taactctagc ccatggttta aatcacttat
     5941 agttgatgga ataattggag gtgttggtgg tactttacca ttcttccctt taatatttac
     6001 attattcttt ggtatatcgt tgtttgaaga tagtggatat atgtcaagaa cagcattttt
     6061 aatggataaa gttatgagaa aggttggtct atctggtaaa gcttttatac ctatagttat
     6121 gggtatggga tgttcttctc cagctataat ggcaactaga acattggaaa gcgaaaaaga
     6181 tagaagagtt acagctctta ttgcaccact tatgacttgt ggagcaaagc tacctatata
     6241 tgcactattt gtagctatat tctttccaca taatgctgct ttagtaacta catcactgta
     6301 tttggtgggt atagttatgg caattgtagt tgcattattc ttaaataaaa cagcctttaa
     6361 aacagaggct gaaccattta tattagaatt accagaatat agaatcccta ctatagatgc
     6421 acttattaaa aatacttgga ataaatcaaa aggtttcttg ataagagttg ttactgtcat
     6481 gtttgcaatg tcagtcgtaa tttggggact ttcttcattc aatgtttttg gatatacaga
     6541 agatataaat gtaagcttct tggctactct aggtcatatt atatctccta tattcaaacc
     6601 tcttggtttt gatgattgga gaacttcagt tgctatttta agtggtcttg gagcaaaaga
     6661 aatcgtagta aactctttaa atatactata tggtaatcta actgttgtct taccaacaat
     6721 atttaatgga gttactgcat atacattctt gatatttact gctttatata ctccatgtat
     6781 agctgcactt gcaactatga aaaaagaata tggcaataaa atgatgttta catcatttgc
     6841 atatcaattt atactagcat ggataatggc ttttatagtt aaaagtatag gtggagtact
     6901 atttatgggt aattcaataa tagaagcttt gataggtgga ataattatag tccttgcact
     6961 ctttattctg tttaataaat tcaagtctac aaaaaacgga aatgtctgta gtggatgttc
     7021 tatttgttct ggatgtccaa gtgctaattc ttgctcaagt aaggaagaca gcaataaaaa
     7081 aaataatgca cactaatata taaatatttt agtttaaaat aagataaaag tgggtgtctc
     7141 aaaattaact ttttagttca tgaggcactc ttttttatag aaaatcaaat atattttcat
     7201 catttcaaca gtaaaaaaga gcttatctag taaaaaagag gcttatctct attttgagac
     7261 aacctctttt tttgttactt attcagtttc ttgctaccta acttcatgtg gaacctagta
     7321 tcttttggaa cacttgggtc tacacctctt aatgtaggta tttccccaca tattagttgt
     7381 ataggtacta taaaattaaa tgtttgatag taaggattat ctgtgatatc aatctttaaa
     7441 tttttatcat tttctgtaac atctctacct atagcaaata cattttccgt ccaaccagaa
     7501 agtacatcaa tcatcttagt tactctaggt tcttttcctg tatctagtat aaatatagta
     7561 gaatcactat ttatagcatt atatatacca tgtataaact cttcaaactc atacccagtt
     7621 acaggtattc tcattgtttc caataatttt aatgctgctt ctaatgtatc tccatatata
     7681 tcagagtgac ctattattct tatttcttta gaattaacta acttttcttt gtttctttca
     7741 atccattgtt tagagagctt atacactgct tcaaatctat taattgcgtc taatatctta
     7801 tttatttcct cattgtactt ttcactagat attatacctt tttctctagc tatttgaaga
     7861 cctaataaca ttaagtttaa ctttgtacaa taatatcctt ttgttttagc tccagatttt
     7921 tcttcaccac aatttactgt taatatataa tcagaaatct catctattaa agcattctta
     7981 caacctgcca tagatgctat tttacagccc ttatcttcag ctaacttcat agcgttgtat
     8041 gtagaataac tactcccacc ttgagataca cctactacta gggtattttc attatcaaac
     8101 ttgaatgtat cttcagttat cataaatgga tacattttca caacatctat atctaataaa
     8161 ttttgtaaat atggttgtac ttgcactcct gagtgatagc tagtacctga acctgttatt
     8221 ataatctttt ttagatttgt tctcttaact tcattaaata agctatctgc attagatata
     8281 atttctctca ttctaactgg tgtttctaac atataatctt gtattgtcat tttatacacc
     8341 ttccttatct atatattcaa taaattataa ttaatttcta taacaaacct atagcatgta
     8401 aagcgattcc tgttactata gtgcctatag ttaattttgt tacacttacc tttctcttta
     8461 acatgtagta tattacaaat gcaaaggtta aaggtaacat attaggtatt attttatcta
     8521 gtatatcttg taaaattact tctgcaccat ttaagttgaa tttcagagga gtagttatac
     8581 tcaacatagt tgcaaccatt cctcctacaa cgaataaacc aacagttgta gccatagaca
     8641 ttattttctc cataagtccc tctctttgta ttctttcaag agaatttacc cctacttgat
     8701 aaccatactt aagtccaaat attctaaggg caaatgctgg tatattgtat aatagtaaga
     8761 ataatagtgg tccaaagata ttaccttctt ttgcaagtga tactcctact cctgctgcta
     8821 taatcctaaa tgttccccag aataatgaat caccaattcc tgcaagaggt cccataagag
     8881 ctgttttaac agcactaatt gaactttcat caaattctgg attattagca ttctcttctt
     8941 ccatagctgc tgttatacct aatatagcag gaactactac tgtcgttgta ttaaaaatct
     9001 ccaaatgcct ttctattgct tttgcttgat tttctttctt attgtatagc ttctttattg
     9061 caggtaacat tgaataacag taacctaagt tttgcattct ttcgtaatta aatgcacctt
     9121 gaagagcaaa tgaacgccaa aacatacttc ttaattcttt ttttgttaat ttattcaaag
     9181 tcaatctctc cttccgttaa agtattgatt gctaattgag gattttgatt attttctttg
     9241 ttattgatat aaaagtttag tacaaatgct acacatactc caaataaagc tatagcagtt
     9301 atatctaatc caccataaga tgctaacaaa aaccctataa agaaaaatac agacatcttt
     9361 tttgaaagta acatatccat aagcaatgca aaacctactg ctggcaacaa tgttcctgca
     9421 acacccagtc cgtctaatat aactttaggt attgcattta tcaatgctgt aactttatca
     9481 gcaccaatca taattgctaa aaaggttgga actgatgtac ttaaaaagaa taatattact
     9541 ggcaaccaca tcattatggt aactttctta aaatcagctt gtttagcata gaaaactgcc
     9601 ttttgagaaa agtacgaatt tattattcta actaatactc caagagattg tgctaagact
     9661 gctactggta ctgctactgc taaagcaact tctgcaccat tacctgataa tatagcaaaa
     9721 gctgtaccta atacacctcc tgttactaca tctggaggag tagctgcacc tattcctgca
     9781 attcccatcc aaattaattc aagctgtgca ccaatcataa ttcctgattt tatgtctcct
     9841 aaaactaatc ctactaacat acctgtaact attggcctat caagcataga ttgaccaaat
     9901 atacgcccat ctagtatacc aactcctgct ataatagcta acatagtagc ttgtactaac
     9961 atattgaacc tccattttaa cttagtttaa tttacattaa attacattac atttttgatt
    10021 ttgttttgat tttctaataa ctcatttaca taaacaattc cattttttgc ttcctctaat
    10081 cctgatttaa ctattagttt tgtatcctcc tcaactaata aaagtataga taaaagcatt
    10141 ggtaaattta ctcctgtcaa taattcaaat ttgaaccctt gtaaaagtaa gtttgtacat
    10201 atgtttgcca cacttccacc aaaaacatct gttaagacaa caaattcttc actatcttta
    10261 ttatttgata atatacttct aatttctttc tcagcatcat ctgctgattt atccgttgtc
    10321 atacaaaaat actcaatatt agttaatgaa ccaattatta atttacttgt gtcaacaagc
    10381 tctctggaca attctccatg tgttgcaatt agaaattttt tcattctctt tctcctaaat
    10441 tattttatta aatctgatag ttgtttcttt ttgtctctag gtaacattct aaattctata
    10501 tcaatcccta aatcacctat ttgtttaaag ttatttatat catcatcatc aacagcaact
    10561 aaatctgtaa gtttcttctt cccttcctca tatcttaatc ctcctacatt tatttctttt
    10621 aatactcctc ctgaattttt agctacaact aaagcatctt tggtattttt tactaacact
    10681 aatacattgc ttttctttga agaagcaaat ttttctacta tttctccaga ttcctctacc
    10741 ccccttatat ataaagttac attgctaggt ttcgctaagt tcagtgacat agctttcatt
    10801 tggtcatttt ttgcttcatc atttgcaact agtattacat ttatacctag tgatgttgcc
    10861 catccatatg caacctgacc atgtatcaat ctgtcatcaa ttctcatcaa agataccatt
    10921 ttccccaaat cctccatcat acattaaaat ttttattgtt tatactctca tttatctata
    10981 aaagcaaaaa gtatgccaaa tactggcata cttttttaaa tttcattcac ttattttgta
    11041 atcttgtatt ttactgtggt agaccataga taatattata aatatatcct atctctgaaa
    11101 ttggtatttg cacactataa ctcttttcta gcacactaaa tgcatttttt attagtttta
    11161 cttcactatg atggtttttg attaacaaat ccatatcagt atactcttca atttcagttt
    11221 gtctaactaa tctttcaatc atacaactta agtgaacata taaagaaatc tttttatcat
    11281 ttgaaagttt tttcttttga atattttcta tagcattaat tccttcttct attctctcta
    11341 ttatcttttt tgaatctaaa atagttagac tatctattac tctaattaat gaaaaattct
    11401 ttacaataga atcatttatg ttctttattt tttcattttc tactactctg ctaaatattc
    11461 taaaaacatc atcttctcct tttcctgata ttatatcctc taataatata aaatctaccc
    11521 cattcacatt tggattggca gttcctacta tagccatcac atcatacatt tcaaaaactg
    11581 gttctttatc tcccatagaa acaagactat cataatcata aggtattatt ttgacatcaa
    11641 ctattccaat taaactttcg gaaagtagat gttggatttt tatagcagta cctatccctg
    11701 taaaacaaga tgatattata gcatattctt tatttttttg aggataatac aattgtatat
    11761 taggtttagt ttcttcatcc ataatttctg caatctcttc aatgttttta ccttgcatta
    11821 ataaatttcc tacttctagt gcagaaatag tggatatttg gtctataaat aacattgggc
    11881 tatttatctt attctttaaa tcttcatata taagattaag agagcccata tcaaataaca
    11941 caattaaacc attctttatc ctatctccct ctaggtatct agacatttgt tttgatacat
    12001 ccgcaatagt agcatctata ggcatatcaa tagctgtaaa tatattttta cccaacatcc
    12061 gattacatac attcgccata ctactggcag tagcatatcc atgtgctata atagtcgcat
    12121 taacttgatt aaatttcatt cttataattg ttgatttaat aaatataatc attatgatta
    12181 tatctacatt attaagatac atatccaatc tattcgttat taatgttgat attttactta
    12241 ttatagagta ttctttgtaa ctctcttttt ttaaatatat tagtaattta ttttctaaag
    12301 ctttatactc atcagacaat tgataagatt cgtccttcaa ataaagataa gacgaaaatg
    12361 ccaatacatt attcccatca tattttatat tgttattaac ttctaaattt ctaaatatat
    12421 cctgcattat cgatattata agctccataa gtacgttatt attttcatca ttatttgaaa
    12481 agactaaatt gtccattagt ctaatcatta gattagaact ctttttacta aatttttcct
    12541 ttgttatttc atttttttct agtctatcaa agtaatctaa acattttatg aaactttgat
    12601 agattatatt tcttttattt cttccatttg acatttcaat actaaattgt ttaggagaaa
    12661 agataatgct tttgctttta ccactttgga attgaaatat atctacattt tctatataaa
    12721 gatttctagg aaggtcttga agagtaacaa aaatatttcc atttcttctt tgattttttg
    12781 cataagctcg tccacatgca tattttatta catttttaca ttcacctata ttccccttga
    12841 attttatctt taaaatcgca tttaatgtaa tttgagttat ctctataggc aaaccaagta
    12901 ccttactttc ttcataaata aaatgttcta caatctttct tttttcaata gtccctcttt
    12961 cttctatatt gggaacataa atatttatgg gtattcttct taaaaaggtt tgtaaaaatt
    13021 cagatagact ttctgttgta gcaaatatta atcttaccct agcttttcta attactccat
    13081 tctctccaat cctagaaaac gtacctgtat ccataaatgt aaataacttt tcctgacctt
    13141 cactatttaa tctatgtact tcatctaaaa ataaaacacc accatctgat tgttctatta
    13201 gacctgctct agattcattt gctccagtat atgctccttt tacatgccca aataatagac
    13261 tagacaataa ctccaaatta ttataatact gcgcacagtt taacacattg tatggagaat
    13321 ttttttctaa tacattactg cttacactat atttgtatat tatcttggct aaaaaagttt
    13381 ttcctactcc actttcacct gtcagcatta ttgtaagtcc gccatttgga taaagtacag
    13441 cggtttttat ttgttcgatt acttctttta agctattatc atatcctatg atactattaa
    13501 acacagtctc ttgctttaat aatttacact catttttaag ttcatctaaa ttttcataaa
    13561 tacttttttt tgtaggtcct actacatcat ttattttatt tttaggtaaa aaaataacag
    13621 gtctactgtt tatctttata agattatcag attttactaa atcatttaat aatctacttg
    13681 attgagttct acttaaatta aactttttta ctatatcttc tgttattatg ccactttctt
    13741 ttttaagttc atcaaagttg ttaaataata cacattcatt tacaaaactt aaaatttcat
    13801 ccctcatgtt caacctccaa tacaaatatc aataatacta taatattagt atatcattta
    13861 tatatttatg aaaaagataa ttatattcct atcttgattc tcttttgtaa gtttgtaaaa
    13921 tcaaactttt tataagtata aaatctagat tttttaacat aatctcaagg ttcttatctt
    13981 tccatatatc tg
//
LOCUS       DAYAOX010000099        13805 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.099, whole genome shotgun sequence.
ACCESSION   DAYAOX010000099 DAYAOX010000000
VERSION     DAYAOX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.099"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(693..1322)
                     /locus_tag="ACGI57_002198"
     CDS             complement(693..1322)
                     /locus_tag="ACGI57_002198"
                     /EC_number="3.2.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
                     /protein_id="HFG9717274.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(1401..2363)
                     /locus_tag="ACGI57_002199"
     CDS             complement(1401..2363)
                     /locus_tag="ACGI57_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9717275.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(3125..6667)
                     /gene="cbpA"
                     /locus_tag="ACGI57_002200"
     CDS             complement(3125..6667)
                     /gene="cbpA"
                     /locus_tag="ACGI57_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HFG9717276.1"
                     /translation="MQQKCKKKALSMILSISMGLGPVASFNASNTFADTIEESTNAGN
                     AEGGTNAGNAEGDTNAGDAEGDTNAGDAEGDTNAGDAEGDTNAGNAEGDTNAGNAEGD
                     TNAGDAEGDTNAGNAEGDTNAGDAEGDTNAGDAEGDANAVVVEEEATAEEPTSVGDFK
                     ISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIK
                     TASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVNR
                     DIEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGN
                     TESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGIITAKGAYHGA
                     GIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPGT
                     NRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGDL
                     EKKTKVFMNKISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQGILYLWFSD
                     QFKGSEVSVNLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDTPPYLIIKR
                     YDGLPFEEDVEKSFLDWIFKDGGIPIITPKGEKLTDKKFMTISSQRLNEETMEPDINL
                     GSDDLTKQVDAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTETSLVVEES
                     KAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKGS
                     ESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDMTNVDV
                     DGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPD
                     KNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPDN
                     GGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDP
                     DNGGNSKPDPDNGGNSKPDPDNGGNSKPDIDTDGDGKPNINIDTDGDGKPDINIDTDG
                     DGKPDINIDTDGDGKPDINIDIDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNV
                     QTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
     gene            6936..7508
                     /locus_tag="ACGI57_002201"
     CDS             6936..7508
                     /locus_tag="ACGI57_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HFG9717277.1"
                     /translation="MKKLVKILINIICIITLIFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEAFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNSNYEFKDYLNYIT"
     gene            complement(8521..9183)
                     /locus_tag="ACGI57_002202"
     CDS             complement(8521..9183)
                     /locus_tag="ACGI57_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717278.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(9200..11755)
                     /locus_tag="ACGI57_002203"
     CDS             complement(9200..11755)
                     /locus_tag="ACGI57_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9717279.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNIRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGMISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLISVVVSNRDFESMNIIEGIKENE"
     gene            complement(11995..12183)
                     /locus_tag="ACGI57_002204"
     CDS             complement(11995..12183)
                     /locus_tag="ACGI57_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HFG9717280.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTK"
     gene            complement(12195..12374)
                     /locus_tag="ACGI57_002205"
     CDS             complement(12195..12374)
                     /locus_tag="ACGI57_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717281.1"
                     /translation="MIHIEISEKEFFRKIEEVFDKGEVNVEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(12379..12636)
                     /locus_tag="ACGI57_002206"
     CDS             complement(12379..12636)
                     /locus_tag="ACGI57_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717282.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKEKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(12609..12749)
                     /locus_tag="ACGI57_002207"
     CDS             complement(12609..12749)
                     /locus_tag="ACGI57_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717283.1"
                     /translation="MVIAISLTLGLITGCTNKVSGTNEKIYTIEQISSIKKTCQRKYQ
                     NQ"
     gene            complement(13016..13222)
                     /locus_tag="ACGI57_002208"
     CDS             complement(13016..13222)
                     /locus_tag="ACGI57_002208"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFG9717284.1"
                     /translation="MCKYLDNAIEACDKIHDKSIDKYIQIKGTISKSYFVIKCENSKI
                     NSIRKNKSLLLTDKKDKLVHGIGI"
     gene            complement(13398..13643)
                     /locus_tag="ACGI57_002209"
     CDS             complement(13398..13643)
                     /locus_tag="ACGI57_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFG9717285.1"
                     /translation="MIPRLILASSLVIIIVFIKIIKENKLKLQNEVIKNKLDMQYSHY
                     LSIQESHAKVKKLYHDINNHIYCIENLKYKKGKLMGM"
BASE COUNT         4452 a         2355 c         1443 g         5555 t
ORIGIN      
        1 acattacaat acagtactaa attagtttat ttcatctatc atctcaatca tatcatttac
       61 taactattat tctaatgagc atataacatt gaaacttata attatacaac ctaaatgcct
      121 cccaatgtac cctcaaaaca tcatttcctg taactgaagt ataattggta tattttatca
      181 ctgaaataaa aaaacactag tcaataaaga ctagtatttt caagtatttt atcattcttt
      241 ttttaattat tggagacgaa tcatcagaat aatgcccata atttatctat atgcaaattc
      301 taaatttaaa ataaagtttc agataaattt actattctat gctatgatac aacagaaaaa
      361 actgaaatct atgagtggta gcttcatata atcctagttc ctacagtaat caaaactgag
      421 gtttattcta tatgtaacta atgataaatt ttattatagg agttattaga tctatttgtg
      481 ttactataag tagtaattat atatatgata attttttaac acttttctgt tggctatata
      541 gaaagtggtt tagaatttaa atttaaataa aatttaaatt caccaaaaag aaatctatat
      601 tagaaaccac tactctattc caccagattg taattttttg ttctttatat cacaatgcta
      661 taccatatta aattgaaaaa taagttttaa atttatttgt tacaattcat taattcaaaa
      721 aaatattttg agtcatgagt tttgtaaaga tttagccatg aatttaatgc ttctgattca
      781 aaaacatcta aaagtttcaa tacttgaaac gtaaattcca acggagcaat tcctgaagcc
      841 gtaactaaat tttcatcact aactacagat tccgttttat aatatttttc tccagagtaa
      901 tttggtatta ccattttaag aaactctaag tcattacttg tatgatttct aaaatctaat
      961 agcccttttt tagctaggcc taatgtagct ccacatatgg ctgcaactat agaatcttgt
     1021 cttaaagctt ctttaacttt ttctaatatt atctcatgat tcatagataa ccaagtattt
     1081 ccccctggca aaattaataa atccttactt tccatattaa actcatcaac tgtaatttca
     1141 ggtaaaactt ttaacccacc cattgttata ataggatttt tatcaattcc tacagtaatt
     1201 acttttagtg gctttaatcc ttttttaaaa tatcttcctg tatttaattc agcagttaaa
     1261 tatgccactt cccaatctga catagtatca aatacataca tatatacttt tctatttttc
     1321 atctttttta ctccttatgt aattaattta tatatctaat tataaaataa cttaactgac
     1381 atattcagtc agttaagcta ttaaatttga taataatcaa ttaattctag taaatcttca
     1441 attattttct tcctaaggct tagtggttca attacttgta ttgtctttct ataaggtaaa
     1501 agtatatttg gtatgtactt gcatattact tcctcttcaa gcataaatat tattttatta
     1561 ggtgttcttt ctttcaagta gtgccctaaa aaccaatgtt ggcttaaact atctaaagaa
     1621 tctatactcc cttctataac taattgaatt atattttgtt catcttttat ttcaggtata
     1681 attcctttta taaaaaattc acttgctgaa aattctgtcg gtctgttaaa tgtatttgat
     1741 gtttctatga tttcactaat cctatccact ctgaaactac gaacctcatt tctcatatta
     1801 caaaatgcaa ctacgtacca attattattc cagtgaacaa ttccatatgg atttataatt
     1861 ctatattttg attttacttt acgacctgca tgatagttga tttctacaga tagctccttt
     1921 aatatactat tctccaattt tttaagtact gattctatag acactttccc aattggtttt
     1981 attacttcaa gggctcttaa gtggtttgtt aaaatatctt cttgtttttg atttgaatac
     2041 atttttaact ttaatatcga tttatctaat gcttcgtcta agaagtatcc ttctttttta
     2101 gcaaatagtg ctgcatgaag tagagatttt tgctcttcta tatcaaaaaa aagaggtgct
     2161 ttagtaaaat tatttaaaag gctataccca ccattatgac ctgtatctga aattataggt
     2221 actccactcg cagaaagtac atcaatatat cggtacactg ttcttatatt tatttctaat
     2281 ttttcagcta tctgttttgc tgtaattttt ttgtcagagt ttaacatcca cagtatagac
     2341 atcatattgt caattttagc catgtagttt cacctctttc tatttgttat ataaagttat
     2401 tatataactt tttgattgat tttttacaaa aaaacattag taaactgcta ataatttata
     2461 ctaagattta tttcaaatat ttatagatag tctttttcta tagttaagtt atccattaaa
     2521 ttacatagta gtatatctat tttagttgat atattttcag ctacctccct tgaatattat
     2581 tacaataagt ctaaatcttc tttcttccat aaatatatta tgaggaaaga ctatcatctt
     2641 taatggttta atagtataaa agatattaat ttaaaaaaat ctatttttat atcagatggg
     2701 tattgttttt tataaaactg aaaaaatcac ctaatagaat ctatgatttt ttaacataac
     2761 ctccataaat tagttccaat tatcacttca aattcaaatt tttatctgaa gaatatacaa
     2821 tcatatttcc atactcaaca taatattgag tgtttggctt gtatttacaa attattattc
     2881 taacaaaaac aactatacat agattaaaat catcttctaa acataactac taaaattgaa
     2941 ttaatgatat caattccatt aatctctttt aataaaaaat tctcttttct atatgaaaat
     3001 tatttcaaaa cttttaagtt aaatacaaaa atgactataa atagattttt atttacacaa
     3061 atctatttat agtcactata gtttatccaa taaaataata aaataaatat gtacacaata
     3121 aatgttattt acgtcttaag tatttatttg ttagattttt aattaaaaaa aacaagctaa
     3181 tgaacattaa tgctaaatct agcattatat tgctttgatc tcctgtttgt acattttgac
     3241 ctacattact atctaaactt tcatctgtat ctatatttat atctggtttt ccattcccat
     3301 ctgtatctat atttatatcc ggttttccat ctccatctat atctatattt atatctggtt
     3361 ttccatctcc atctgtatct atatttatat ctggttttcc atctccatct gtgtctatat
     3421 ttatatctgg ttttccatct ccatctgtgt ctatatttat attcggtttt ccatccccat
     3481 ctgtatctat atctggtttg ctatttcctc cattatctgg gtctggttta ctatttccac
     3541 cgttatctgg atccggtttg ctatttcctc cattatctgg atctggttta ccattgccac
     3601 cgttatctgg atctggtttg ctatttcctc cgttatctgg atccggtttg ctatttcctc
     3661 cattacctga atccggtttg ctatttcctc cattacctga atctggtttg ctatttcctc
     3721 cattacctga atctggtttg ctatttcctc cattatctgg atctggctta tttagatttg
     3781 gattacctgt atcataacct cctgagccag tatctacatt tttatcaggc ttccaataat
     3841 catctactcc atcattatct gtatctattg ctggacgatt ataatctgta tcagggtttc
     3901 catctccatt ggtatctata tttttatcag gtttccaaat accatctcca tttccacctt
     3961 ctgtactagg cttccattca cctgtattat cagtatctat gttaatatct ggcttccaat
     4021 caccactcgt atctatattg atatctggcc ttccatcacc atcagtgtct atatttatat
     4081 ccggtattcc atccccatct acatctacat ttgtcatatc taactttaac ttcttttcat
     4141 cctcactacc ttcaatgtaa tttatatcat ctccaatata ttttacagta atagtttgtt
     4201 cagtagtaaa aatatgatga ttgctatcta aaggtctcca ttcaatagat gcagtactat
     4261 aattatgact ctcactaccc ttattatgag tttttaattc tactggatct ttatattcct
     4321 ttccattaat atagaattgt acgtaacctc ttggagaggc acaatctggt ccttctttct
     4381 tatctggtga aatagtaaca tttaatgtaa acgtatctgt tggtttaaaa acatcagttt
     4441 tagctttaga ctcttctaca actaatgaag tttctgtatc agcaggaata atttctgcct
     4501 tataatatgc tctatgcccc caataagcat ccttaaaagg atatatactc gaaaactgtg
     4561 ttgatgtaat gcttaattga tatttcccag catcaacttg ttttgtaaga tcatcacttc
     4621 ctaaatttat atctggttcc atcgtttcct catttaaacg ttgagaacta atagtcataa
     4681 attttttatc tgtgagtttt tcacctttag gagtaattat tggaatacca ccatctttaa
     4741 atatccaatc taaaaatgat ttttctacgt cttcttcaaa tggtaatcca tcatatcttt
     4801 taataatcaa atatggtgga gtatcatcaa tagtaaattc ttcatattgc tttaaaaatt
     4861 gagaacctga acctatatct ttaatataaa aatcatctgg ttttagaact tctccagtaa
     4921 ttttatctat aacaactaaa ttaacagata cttcagatcc cttaaattga tcagagaacc
     4981 ataagtaaag aatcccttga gcatctgtat aactaggtgc tccataagga taaatttttc
     5041 catcaattag cattttaaaa tcatcaacta gtgtagtact aactttatct tgccctaaat
     5101 acgttagaga tattttattc ataaaaactt ttgttttctt ttctaaatca ccgtatgcaa
     5161 ctgctccacc taaacttttg aatgttgttg cttttaatga accacctgtt actataacat
     5221 ctcccgttgt agcacttagg tcaaaaatcc aatctcccac tcctgtcata taaggtaaaa
     5281 tcgttcctcc agttacaagt atacgatatc ctctatttgt tcctggatta acacatccat
     5341 ccccaaatgc actaccatgt gcacctccat atgaatagga aagtccacca ttaattgtaa
     5401 tattcccact ataaccagaa cctgtgctag gatctactac tgagggactt aatgcattct
     5461 ttgttgttgt atagtatcct ccacctattc cagctccatg gtatgcacct ttagcagtta
     5521 taatacctcc attgattgtg atatctccac cactaccatt acctcctcca cctattccag
     5581 ctccatatcc tgtattacga ctcgattctt gacctatagc agttatagca ccaccattaa
     5641 taataatatt tcctccactt tcagtatttc caccaccaat acctgcagaa taataatttc
     5701 caacaacttt taaacttcct ctattattac tatctagtag tgtaaggcga tctccttttt
     5761 ttaacttgtc tccattttct agagttacat ctctaggaat ttttccaccc tcaggaacta
     5821 taaactctcc ttcaatatct ctatttacaa cagaatcatc aataattaga gtagtacctt
     5881 ttcctgctct catacctgga taatatacat cacctgactc tagcttgttt tcactaccat
     5941 cttgtaatat aacatgtgca tatgcaccac catctttatc tggtgttaaa gtaaatgggc
     6001 tattagatgc agttttaata tttactccat caaatattaa ttcagctttt acatctttag
     6061 gtataacaat cctatgtgtt gttggagtat ttggattgtt attttgaatt gtcatttgtt
     6121 ttccactaag aatagttaaa acgccacttg caaatttaaa atcgacattt atttctccac
     6181 cactaatttt aaagtctcct acagacgtag gctcttctgc agtggcttct tcttctacaa
     6241 ctactgcatt agcatctcct tctgcatctc ctgcattagt atctccttct gcatctcctg
     6301 cattagtatc tccttctgca tttcctgcat tagtatctcc ttctgcatct cctgcattag
     6361 tatctccttc tgcatttcct gcattagtat ctccttctgc atttcctgca ttagtatctc
     6421 cttctgcatc tcctgcatta gtatctcctt ctgcatctcc tgcattagta tctccttctg
     6481 catctcctgc attagtatct ccttctgcat ttcctgcatt agtacctcct tctgcatttc
     6541 ctgcattagt actttcttct atagtatctg cgaaagtatt tgacgcatta aatgatgcta
     6601 ctggtccaag tcccattgag atacttaaaa tcattgatag tgctttcttt ttacattttt
     6661 gttgcataaa ctccccctcc ccttaataat ttttaatata cactgtatac atccttatat
     6721 acactctaat aattcttaat atacgctgta tacattctaa tgcacactct aataattttt
     6781 aatatatact taaaacttat atttgtcaat gaatatactg ttttttcaac cttatatatt
     6841 ataagtattc attatttgct aaataactac aataaaaatt ttaattatat aaaatttatt
     6901 taatttttta tataataata ctaaggggta attaattgaa aaaactagta aaaatattaa
     6961 taaatattat ttgcattatt acgctaattt tctctagcct ttcgatttac ataaaattat
     7021 ctgaatataa aaaagctgat gaagtatata cggaactcag agaaaacact atcaataata
     7081 gcaaacatca agaactttat gataaaaata atgattatag attctggctt aaaataaaca
     7141 acgtaaatat agactatcct gtagtacaag gatataacaa tgatttctac ctaactcatg
     7201 atttttataa aaattatctt cctttaggta gtattttcat ggattataga aataattttg
     7261 aaaatgataa gagtttaata gtctatggtc attatatgaa aaataaaact atgtttggtc
     7321 agctagaaaa ctatactgat gaagcatttt tcaaagaaaa taacttagta gaaataaatt
     7381 ataaagtaca aacttatact tatgaaattt tttcagtata tactgctgac ttaattaaca
     7441 gagattattt aagtatacac tttaatagta attatgagtt taaagattat ttgaattata
     7501 taacctaaaa atcagtacac aaatctgata taaaggtaga tatttcagat aaaattataa
     7561 ctttgtatac ttgtaactat gaatttaaag atgctcgtac tatagttcat gctaaattaa
     7621 tttcaaaaaa agagactgat aagtaggttt gtgtaaatgc agacttacct acagtctttt
     7681 ttgaacgtaa aattagtaag tatggaataa tattcatatt aaacttttgt aggcaaagaa
     7741 atgaaaaatc aaagagaaat aattattaca gatttagatt accaagctga attaagaaag
     7801 tgtaaaacta tggaggatgt agttggtgaa gatagtatat aatagtttaa cttataaatt
     7861 atcttggtga tattaatgaa gtttcaaagt atatattatt tattgatata aataatatta
     7921 aatctgttgc taacagatgt acctttgttt ggagacaaac tatttaagaa atataactaa
     7981 aatgttgaag tagctacctg actttatttt gaaatgcgaa caagattttg gcattaagat
     8041 aatttagaaa gaatcactgg tacacaagga tgtgaactta gaatgaatag aagtattcag
     8101 gcataaggag ctttcaccca aataaaacat agtatgaact ttaaaagaat tctaatcaga
     8161 ggcaatcaaa atgttctttc atagtttata cctgtagtgt tagacatagt atttataagt
     8221 ttcacaaata agataatcat taataaatgt agtatgtata ttcatcctct caaaaatatt
     8281 ttttaatctt acactttgta aattattcta ttttaaaatg gtatttttat acctttattt
     8341 cttatgaccg ttttcaagct caaattttgc ataaatataa attaatgaaa agcaagtaaa
     8401 aaaagaactg tcgcacagca atttaaaatt actcattcga cagtccttat ttcgtggatt
     8461 taaatagtgg agaagaatca caagaataat acccacgatt aaacctttat acaatacatt
     8521 ttatatgact ttaccatcac taatagttat tatacaatca gccatcttag ctatttcatc
     8581 attatgagtt atcataacca acgtctgctt gtattcattt acacaatact ttagtaaatt
     8641 catcacctca ttagttgttt tactgtctaa atttccagta ggttcatctg caaaaattat
     8701 tgatggctta ttggcaagtg atcttgctat tgcaactctt tgttgttgac cacctgataa
     8761 ttcattagga aatttattaa tctgactttc taatcctaac ttagttatta aatcattaat
     8821 ataattatta tctatctttc ctttagcaat taaaatagga agaactatat tatcataaac
     8881 attgataaca ggtattagat tatagttttg gaatataaac ccaaattctt tacttctaag
     8941 attagatagc ttttcatcac ttaaagtata taaatctaaa ttatccataa atacattacc
     9001 taaagttggt ttatcaagcc ctgccataca gtgaagtaat gtgctttttc cagaaccact
     9061 ttgtccaatt attgcactaa atttatgggg ttctatctct aaagtaattc catcaacagc
     9121 ctttatttca ttttctcctt ttccatatat ttttttaata tctataactt ttaacatctt
     9181 catacattta cctcccaaat tattcattct cttttatacc ttcaataata ttcatacttt
     9241 caaaatctct attacttaca actactgata ttaaacatac aaatacagat atagcaaaga
     9301 atagtaaaga ttccttaatt gggaatgtaa aagacataaa attctctatt ccatagttta
     9361 tatacgcatc attattccaa tttgtaaatt taactagatt tataaatgat ggaatcatag
     9421 ctattataaa gctaaaaact gcatatgcta atgcttcata tataagcatt tttttcatat
     9481 ttttaagact aacacctaat gctctaagcg ttgaaatttc tttttttcta attagtagat
     9541 ttgttcttat agtacagaaa atattaattg atgctattat taaaactagt gcaattattg
     9601 ctatttgtga tttaatgtaa cttttatata tactttctga catttcatga taacttaatc
     9661 tactattaaa tagagaccct ggtatacttc ttgaaatttt ctgtatctca ttattaacaa
     9721 tctcatcata tggattttct aaatttatgc ctaattgagt gtatttttgt tctcctgtta
     9781 tgtttttaga gtgaatatta cttgttataa tttctagatc tggcataaaa tcgccatctc
     9841 ccatagcaat ccattcatcc tttaaaatta cagccactct taccttcatc tctttataaa
     9901 caattgtatt attttcttca acaggaattt ttacagttat tatgtctcct atatttaatt
     9961 ctttgaatat aggctttata gtatgatcat caactatatc ataaaaataa ttatatactg
    10021 ctacatttgg atatccatca gtgtcctttt taatatcatc taagctacct tcttcaacaa
    10081 agtcatttaa cttttcatct atataatcat catcataaag ctttaaaact aatttatctt
    10141 ccattatctc ttttttttct actccattat aggttgtaaa ttctttgttt atatccttat
    10201 tattaaaagt aaacattcca tttgttattt gaattttatg tatactttta acacccttaa
    10261 ttttttctat attctctaca tcactatctg tgtattttga tatttgcggg tctgcattta
    10321 ctccaaattg cattgttata ttatatctat tttgcatatc ttcaatttta ctgttgacct
    10381 cttcttgcat acttgagaat gtagtaatga atatatatcc tcctaaagca atagacaaca
    10441 tagatatagc agctctaacc ttatttcttc cgatattttt aagtcccata aatccataaa
    10501 atccaaatat tttgcttact agtctgtcta ttccaagtct acttctgtga tttttaattt
    10561 tgtctgtatt tattattgat tcaataggtg atatcttgcc gattttaaaa gttggtataa
    10621 tacaagatat aaaaacagaa atcattccaa ccaatatagc ttttataact atttcattat
    10681 ttatatataa tttagggtta agaccagctt cactgtataa aacttcgtta taattactaa
    10741 ttcctatata tgaatatgta accccagcta ctaacccaag tagtatacct attattgtta
    10801 taattaatgc ttgatagatt atcatacacc ttatgtttct tttctttgcg cccatcagtc
    10861 taagcagtcc catttctttt actgattgat ttagtgtaat attgaaaata ttaaatatga
    10921 aaattataga tactgtagtt attaaaacat ttcttttaaa aacattcgga tcattcttat
    10981 catcttctac ataacgaagt tcccttacaa gaggtacatt tggtagaaaa cttttttgtc
    11041 ccaacttata ttttttcata agataatcac attcaccttc aatatttctc catccacttt
    11101 taaaacttaa tatactatta taactaatcg catcatgagg tataatgtta tttgtttcat
    11161 cattgccata agttactgct tcgtattgcc aaaccacatc atagtaccct tttggttttt
    11221 ccataactcc tactatttta aattctttat ttatagaaaa taattgatta cttccgttag
    11281 tatctttata gttctttttt attgttaaat ttaatgtaga tcctactcct tcacttaaat
    11341 tcattgcttc tagtgctttt ttctctaata ctatttcatt gttgttttta ggtagtctac
    11401 cttctattat tttgttgtat ctacttttta gatactcttc gtttgatgaa gttaatataa
    11461 atgagtttcc ttttttatct gaaatacgtc ctagattttg taccgaatta ccctttgata
    11521 ctttttcatc agtttctaat tttttagcat catcaagtgt caaatcacta aactctacat
    11581 ggtatccact gtaaagttca tgtattgcta ctacattgtt ttcattttgt gaatctttta
    11641 taacactagg tccaaatatc aacataacac ctaacataac tcctattatt aaaagtaatg
    11701 ttctaagttt atttttcttt aaatatgcta gtgacatttt taagtaaatc cccatataat
    11761 cctcctattt tactataaat tatttatcca actcatctgt taagtagatt ggtggatatt
    11821 ttaaatcttt taattcttca ttattttttg aagcttacta ccatcttcct catgataaac
    11881 ttgcagtatc accttattta catgtagtgc ataataatca ccattataaa aatattagta
    11941 tttataacct ggataatttt tgtaaaatga tttataaagt atcccatcct aattctattt
    12001 tgtatacttt tcttttgtaa tacgctctat atcatcacct gaaactcctt gatatgtcat
    12061 atatttatct atatattttt cagcttcatt ccattttcta aaatagttgt tgtatataat
    12121 aaatcctatt atggctataa tgcttataat taaagttaat aaaatagctt tgtattttct
    12181 catagtgaat cttcctactt caatttaaat tctttaataa gttttaattc taaagactta
    12241 gttttatcat caaatttctt aacttgaatt tttatatctc ccaaattatc aacctctaca
    12301 tttacttcac ctttatcaaa tacttcttca atttttctaa aaaattcttt ctcacttatt
    12361 tctatatgaa tcaatgatct atatgttata aatggccatt atgaatttat catcttctga
    12421 cagcttttca ttataagaaa aatctttaac acattgaatt gataatattt ccttcgtatc
    12481 cttcttttcc ttagttattg aataatgtaa aacttggcta ggttgttctt ttgtatactc
    12541 caagtttccc aaagacaaaa caaaacttcc atctgattta ttttcagcta ccctaagacc
    12601 actattttct attgattctg atattttctt tgacaagtct tcttgatact agaaatctgt
    12661 tcaatagtat atattttttc atttgtacct gaaaccttat ttgtgcatcc tgttattaaa
    12721 cctagtgtta agcttatagc tataaccata gttattgtaa tttttttatc ttcatataat
    12781 cctccttata tattgaaaac tcgttatttc tcataaaaaa aagacgctat ctttttctat
    12841 taaaagataa catccctata tgtttagtat caatatatgt tccccaactg gtaggttaat
    12901 cgacccaact atcaaaattt tgatttaatg gtatataaat attcaatata aatttgtctt
    12961 ttttatcttc aaatgataaa tctcctctat atttttttat agaagatttg atgctttaaa
    13021 tccctattcc atgtacaagt ttatcttttt tatctgttaa taataaactt ttattctttc
    13081 taattgaatt tattttacta ttctcacatt taattacgaa ataggacttt gagatagttc
    13141 cttttatttg tatatattta tcaatacttt tatcatgtat cttatcgcaa gcttctattg
    13201 cattatctaa gtatttgcaa atatattaga tacatctatt ggttttataa aattcacttt
    13261 ggaaaaattg atatcacaaa tgaatttaat ttttcttttt tcacttacat cacttttttc
    13321 atttattatt atatccaata taacattacc tgtattgtaa attgatttaa attctttaat
    13381 ttaatctttt aaactattta catacccatc aattttcctt ttttatattt gagattttct
    13441 atacaatata tatgattatt tatatcatga taaagcttct taactttagc gtgtgattct
    13501 tgaatactta gataatgaga atactgcata tctaacttat tttttattac ttcattttgc
    13561 aattttaact tattttcctt aattattttt ataaacacta taataatgac taagctacta
    13621 gctagtatta acctaggtat cacaatatac ccaactatat atatttccta atacccacca
    13681 aatcgataca tttttctatt tatataaatt attaatatag taaataaatt tgttattaaa
    13741 gcaacagata agtatatata gtttatttta ttatctttat ctagtcccaa tcttttatta
    13801 ataaa
//
LOCUS       DAYAOX010000100        13707 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.100, whole genome shotgun sequence.
ACCESSION   DAYAOX010000100 DAYAOX010000000
VERSION     DAYAOX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.100"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            126..788
                     /locus_tag="ACGI57_002210"
     CDS             126..788
                     /locus_tag="ACGI57_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFG9717286.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            1159..2568
                     /locus_tag="ACGI57_002211"
     CDS             1159..2568
                     /locus_tag="ACGI57_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFG9717287.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            2683..2958
                     /locus_tag="ACGI57_002212"
     CDS             2683..2958
                     /locus_tag="ACGI57_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HFG9717288.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            3588..3899
                     /locus_tag="ACGI57_002213"
     CDS             3588..3899
                     /locus_tag="ACGI57_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717289.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            3901..5826
                     /locus_tag="ACGI57_002214"
     CDS             3901..5826
                     /locus_tag="ACGI57_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFG9717290.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            5829..6320
                     /locus_tag="ACGI57_002215"
     CDS             5829..6320
                     /locus_tag="ACGI57_002215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFG9717291.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            6372..6935
                     /locus_tag="ACGI57_002216"
     CDS             6372..6935
                     /locus_tag="ACGI57_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFG9717292.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            6950..7927
                     /locus_tag="ACGI57_002217"
     CDS             6950..7927
                     /locus_tag="ACGI57_002217"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HFG9717293.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            7920..8237
                     /locus_tag="ACGI57_002218"
     CDS             7920..8237
                     /locus_tag="ACGI57_002218"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFG9717294.1"
                     /translation="MYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVEDEY
                     AIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGADIL
                     FKE"
     gene            8354..10132
                     /locus_tag="ACGI57_002219"
     CDS             8354..10132
                     /locus_tag="ACGI57_002219"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFG9717295.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            10129..11502
                     /locus_tag="ACGI57_002220"
     CDS             10129..11502
                     /locus_tag="ACGI57_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFG9717296.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            11508..12176
                     /locus_tag="ACGI57_002221"
     CDS             11508..12176
                     /locus_tag="ACGI57_002221"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFG9717297.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            12453..13502
                     /locus_tag="ACGI57_002222"
     CDS             12453..13502
                     /locus_tag="ACGI57_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9717298.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
BASE COUNT         5277 a         1666 c         2637 g         4127 t
ORIGIN      
        1 tataaaaatt atattaaaaa taaatttatt ttgctaataa acatgttatt aacaaaagaa
       61 tagtactata atgtatagat gataaaaact ttaataaaaa tttagtgtat aggagttgtt
      121 taagtatgat agatataagt attgatgaca aacttaagga aagatggcct aatgctaagc
      181 tgggttgcat tcaagcaaaa gtagcagtaa ataaaagctc agaaaaatta attaatgaaa
      241 taaatgagtt ttgtgatact ataaatcaaa gtctaaaagt tgaagacatt acgaaactag
      301 ataaaattaa agatgcaaga aatgcttata aagagcttgg aaaaaatcca agtagatata
      361 gaacatcttc agaagcattg gttagaagaa ttattcaagg gaagggtctt tatacaataa
      421 ataatatagt agaaataaac aatttaatat caataaaatc attatatcca gtaggtacat
      481 ataatgtaag caaattacat tcaccagtat gttttactgt aggagatgaa ggagaacagt
      541 ataagggtat tgggaaagaa ttaataaata tagaaaatct tcctatatta tctgattcag
      601 tgggtaaatt tgggagtcca actagtgatt ctgaaagagc aatgataaca gaagatgcaa
      661 atgaaatatt aatttgtata ttttctttta gtcaagaatc tgatattgaa gaatattttg
      721 gatatgctaa ggaattactt aaagaatatg cagatggaaa agatatagaa actaaaataa
      781 ttaaatagaa tctatataac aacgttatgt taagctaaaa ttcaatataa aaataagtta
      841 agataaagaa actaacacaa aattagtttc ttttttaaaa tagtagttta gtgttacaaa
      901 aatagataga tagtcccacg attattaaaa gtatggtaat atattaatat agcagagatt
      961 gtgtttaagg gattttgaaa ggggggattt ataataatca ttttatctaa aagataaaat
     1021 ggttttatag aaataactat atatttaaaa atataaataa tgatacagta atttgggtat
     1081 ataaaataag gcatacatgg ttttgtttaa aaaccaataa ggtacatagg taattacaat
     1141 gtaaggaagg atttaattat gattgatgga aaagaggtaa aacaagaaag tggacaaaaa
     1201 aatctaaaat ttaagatagc tatagtagta ggtgttttgt ttgtagctta tataggaatg
     1261 gcaatctttt ttagaaatca tttttacttt ggaacgacta ttaatggcgt aaaggcttca
     1321 ggaaaaacag ttgagcaagt aaatgatttg ttaacatcta gtactaattc ctatacttta
     1381 aacttagagg aaagaaacaa taagaaagaa caaataaaag caaaagatat tgatttaaaa
     1441 tttacaccag atgataaagt tcaaaagata aaagatagtc agagcgctat tggatggatt
     1501 ggtggaatat ttaagtctaa agagtataat aatatgataa ctctttcata taataaagat
     1561 ttactagaaa aaaggtttaa tgctttatct tgttttgata gtaaaaatgt tgtagaacct
     1621 aagagtgctt atcctaagta tgatagcact aaaaacgctt atgttatagt accagaggta
     1681 cttggaaaca aagtgaaaaa agatgatttt tataagttag tacaaaactc aattaattct
     1741 gggaatactt ctatttctct ggatgaaagt aaagtttatg aagcaccaaa caacacaaag
     1801 gattcagaaa agttgaaaca agctattaag acattagata agtatgttgg tgtagtcgtt
     1861 acatatgatt ttagtgaccg taaggaaact ttagatggtt ctgaaattaa taaatggtta
     1921 aaagtagact cagaaaagaa ttatgacata acttttgata atgatgctat ggtagaatat
     1981 acaagaggtt taagtaaaaa atacagtact tttggtgact caagaccatt taaaactgcc
     2041 acaggtaaag atattacaat ttcaggtgga atttatggtt ggttgataga taaaaagaaa
     2101 gaagctgaag cattagttga tgttgtaaaa gctggtaaaa atgttacaag agaacctatt
     2161 tacatacaaa aagctgtatc tcgtaataaa gatgatattg gaaatacata tgtagagatt
     2221 agtctttctg gccaacatat gtggttcttt aaaaatggag aaatgcttgt atcaacagac
     2281 gttgtaacag gtgatttagc tagaggattt gctacaccat caggtatata tcctcttaat
     2341 tataaggcta gaaatgtttc attaactggt caaggttaca gttcacctgt aagttattgg
     2401 cttccgttta atgggaacat aggcattcat gatgctactt ggagaaattc ttttggtgga
     2461 agtatatata agtcttcagg ttcacatgga tgtgtaaata cacctttttc taaggcaaag
     2521 aaaatatatg aaaatataga accaggtaca ccaattattt tatactagta aatgtgttgc
     2581 taacagttaa gcgaagtcat cttagtttat ttaggatggc ttccttttta tataaagctt
     2641 attgaagttt acagaaagac tacttaaaag gagaaataca taatgaatga aaaattaact
     2701 aaaattttag aagaagctaa gaatgatgat gaatttagaa aagaacttat aaaaacaaga
     2761 gaacaacgtg atgttatgga ttcattttgt aaggttgtta catctagagg ttatgatatg
     2821 acagtagggg aacttttttc tatgggagaa gaatatacat ccaatctact gaaaagtgta
     2881 aatggaggag cagcatatcc tattgaagat tggagtgatt ggtacgagga tttttttata
     2941 atgttgtcga atatatagag taactaatga tattatctac ttaagattaa tacattgcct
     3001 taaaataaaa ctattagaac taggtgtaag ttttatcaat ctttaaaaat attgatttat
     3061 ttctaactag atataaaaaa attagattaa aaataaggtg acaaaaatcg atatagcttg
     3121 atgatttata aaagttagat aaaatagtgc gagatatttc taaaaattca aaaaaaacaa
     3181 taaacttcaa tggttagagg ttgaaaaaga atataaaaaa cttttatatg taataaatta
     3241 aattaaaatt ataaaaaaaa taaaataaaa atttaaaatg tcgacataac gttaatttta
     3301 aaacaaaata acgctattga caaactatat cgctttaaat tataattatt gaaaataatg
     3361 gtatttaaaa accatactca tgtcatagtc taaaagtaag tcaaaaaagg atgtaaatct
     3421 aggcaagtac tattctaaaa tatgttttat taagaaataa acgttgaaaa atattaatta
     3481 ttatataaaa atgctgttta tactttaaca atgaggtagt aatctaatta gaataaaaaa
     3541 atctaattgg ttatatacat agtaaaaagg taagagaggt gatttggatg gcttttgaaa
     3601 taataaaaag cattgttgag gcagagcaga cagcagacag tatcaaagta aaagctgtta
     3661 ctgatgcaga gtctatcaga gctgatgctg taaacaaatg tgaaagcata tttgctgatg
     3721 taaaaaaaca agcaaagctt atggaagaaa ctcttattga gaaggcagtc accgacagta
     3781 gagcagaggt tgataaaatc ttagctaatg ctaaaagtga atgtctgaaa attgaaaaaa
     3841 ctgctgaaga aagaaaaagt aaggctattg aagcggttat tggaaaggta gtgagataat
     3901 atggcaatag ttaatatgaa caaaatatct attgttggct tagaagctca aaaaagccag
     3961 attttaaagc tacttatgaa ccgtgggttt gttcagattg atgatagtgc ctttcttact
     4021 gaggaggagg aattaaaaaa ttatcttgta aaagatagtg aagaatctga agttataatg
     4081 ctagagcaga aaatgtctca tgtaaaccaa gcaattaata ttgtttcttc actagtgaaa
     4141 caaaaaaaat ccctacttgc accaaaaaga gagtttaaca aaattgatag tgaaaaagaa
     4201 gggcaagttt atgctgaagc tctggagtta aacaatttgc acaaggagat aggtacaata
     4261 aggtctaaca taaacttact taacaacaat aaaagtatgt tagaaccatg ggtgaacttt
     4321 gatattcctc ttgaaaatat ggaaactaag tatacaaaaa ctatacttgg aactgtgccc
     4381 ataagcgcaa acattgatgc tttaagagta aaattagaag atgaaatacc agaaagtgtt
     4441 attgggattg tcaattctga caagttaatg aactatgttt atttaattac tttaaaagaa
     4501 acttttgatg atgttttaga agttttaaaa gaatttaatt tttcagtggt ttcattacca
     4561 aatgaagaag gtacaccgtt acaagctatc aagaaataca atgaaatgat aattagacag
     4621 gaaaacgttt ctaaggacaa aacagaggaa attaaaagac atgcagatag tatacacatg
     4681 cttgaaaacc tatatgattt ttacactata gaaagagacc aaaaaaagat tatagagaga
     4741 cttgtcaaaa caaaaacaac attctgtctt aatggttggt taccaagcaa aagggcagat
     4801 gaattaatta aagaaattac agaaaagttt aattgttgtg ttcagactga agaaggaagt
     4861 aaagaagaag gcttcccaat tctgcttgaa aacaataaat tagtaacacc ttttgaaagt
     4921 gtaactaaca tgtacagcta tccaagtaca aaagatattg acccaaatac aattttaact
     4981 ttcttctttg ttgtattctt tggaatgatg ctaagtgacg ccgcatatgg gttaataatt
     5041 gcagtagtct gtggatttgt tgtatataaa ttaaagatac aaaaaggtga aggaaattta
     5101 attaaactta ttggtatctg tggagtttca actactgtat ggggtcttat attcgggggt
     5161 attttggggg atttaattcc tataaaagca ttaattaatc ctttagagga tgttatgaca
     5221 cttatgggaa tgtcgttact gttcggtatt atccatatat atgtaggttt gggaataaaa
     5281 gcatatacat taataagaag tggaaaggta atagatgcaa tctttgacat aggtttttgg
     5341 tatttgtgta taacaggtgt atgtctactt ataatacctt ttgttgcagg ggatataggt
     5401 gtattttcag aagtaggaaa atatttagca attataggtg ctataggtct tgtacttact
     5461 caaggaagaa gctttaaagg tattcctgtt aagcttttta aaggattttc aagtctctat
     5521 ggtataacaa gttattttgc tgatatatta tcttatacaa gaattatggc gctttgttta
     5581 acaacaggag ttatagcaca agttatcaac cttttaggag ctatagctgg tccgatactt
     5641 gcagttgtaa ttggtgttgt aggacacaca ataaaccttc ttataaatgc actaggtgca
     5701 tatgttcata caagtaggtt acagtatgta gaatttttca ataagtttta tgaaggcgga
     5761 ggagttcctt ttgttccgtt taagtataaa acaaaatata caagcattaa taaaaaggag
     5821 atgtaaatat ggaattttta aacgcgttag gaaatggtca atttttggca atatcaggag
     5881 ctgctatagc agcattattt gcaggtatgg gttctgctaa aggtacttca atagcaggtc
     5941 aagccgcagc aggtgttgta acagaagacc ctagtaaatt cggtcaactt ctagttttac
     6001 aacttcttcc tggtacacaa ggtctgtatg gatttatcat agctttcctt attttatcaa
     6061 aagcaggaat tattggtgga gatgcaatac caacatcagc tcaaggttta caacttctta
     6121 tggcaggact tccaattggt ttagttggtt tagtatcagg tatagcacaa ggtaaagcag
     6181 cagcagcagg agttggaata gttgcaaaaa gacctgaaga acttggtaaa gctataacat
     6241 ttgcagcaat agttgaaaca tatgctcttt taggactact tgtttctttc ttagcttaca
     6301 atggaattgc aattggttaa ttataaatta taattcataa gtaaaaaaaa tgaaaaggag
     6361 gaagcactcc tatgggtaat gaacaaaaaa tgattgacag aattatagct gatgccaaac
     6421 aagaagcaca agaaatattg gataaagcta aaagtgaagc agatttaaaa gtaaattcag
     6481 ctaatgaaaa agctgaaaag gaaatggctt cctatacaaa gcttgcagag gcagaagcag
     6541 aaaaagcggc ttctaaggaa atatcaggag cttatatgga ggctaaaaag caaatattat
     6601 caaaaaaaca agagatttta gaagaggtaa ttttagaagc taaaaacaaa cttttaaatc
     6661 ttaaagataa tgaatatgaa gagattatac taaacatgat tgaaaaatca aattgtacag
     6721 atgattcaga gatagtatta tctaaaaaag ataaaaaaac attaaaagat gtgctatcaa
     6781 aaaaaggcat taaagtgtct gatgaaacta gagatattac tggaggtttc atagttaaaa
     6841 aaggtgacat tgagtacaat tattcatttg aagccataat tgcagtagag catgaatata
     6901 ttgaacaaat tgcagcagaa attttgttta attaaggagg ggttaaaaaa tggcagaaga
     6961 aaaaacttat ccctatgccg ttgcacgaat aagagtgctt gaaaaacagc ttcttaacag
     7021 acaaatgttt attcaaatgg cagaagccaa aagccctgaa gactctttaa gaattatagc
     7081 tgaggcaggt tatgctgata catcaaataa taatatacat aattttgaga atattttgac
     7141 tcaagaatta tcaaagacat atgaaattct taaaagtcta gctcctgaag aaaaatttgt
     7201 tgatgtattt ctttataaaa atgactatca caatttaaaa gtgctgatta aagaagaaat
     7261 ttctggtgta gatggtgaga aatatttaat tgatggaggt acaattcctc ttattaagct
     7321 tagagaatct ttagcaaatc gtagttttag tgatttacct aagataatgt catcagctat
     7381 attagaggca tttgatgttt ataataagac acaaaatggc cagatggttg atattgttct
     7441 agataaagca acctttacca gtatgaaaga aactgctaaa gaaagccaaa acaaatttgt
     7501 aatagattat gttatgaaag tttgtgattt aacaaatcta aagagtttta taagagtcaa
     7561 aaatatgaag aaagattttg atatgtttat gaatgtgttt gtttcaggtg gctcacttga
     7621 taaggaaaaa ttcttagaag ctttttcctc agatactcca gcatcatgtt ttaaatctac
     7681 ttcttactct gatgtctgta aaaatggcat ggatagtggt tttactgtct ttgaaaaact
     7741 ttgtgatgac tatcttatgg aatatgttag aggagcaaaa tttaagcctc ttacattaga
     7801 accactaatt gcatatttat atgctaaaga atcagagata aaaacaatta gaattatttt
     7861 gactagtaag cttaataata tagacgcaga tactataaaa gaaaggctaa gggatgctta
     7921 tgtataaggt aggagtagtt ggagataaag atagcatcat gggattttta gctcttggaa
     7981 tagatatctt cccagcctat gatagtgacg aaattaaaaa aagtatacat aagttggttg
     8041 aagatgaata tgcgataatt tatattacag aacaggcatc attgcttgca aaagaatcaa
     8101 tagcaaaata caaagattac caacttcctg ccattatagt tattccaggt attggaggaa
     8161 gtatgggatt aggtatgaat gaagtaagag aatctagtaa acgtgctata ggtgcagata
     8221 tattatttaa agaatagatg aaagttgaat aaaagacaat ctcaaatttt attggtgcta
     8281 aatatatgtg ctaataaaat ataggaggga atatttaaat tttgataaag tatatgattg
     8341 caggtgagag aatatgaaaa caggcacaat tgtaaaggtg tcaggaccgc ttgttgtagc
     8401 ggagggcatg agagatgcga atatgtttga cgtcgtaaga gtttcagata aacatcttat
     8461 aggcgagatt atagaaatgc atggagataa agcttctata caggtttatg aggaaacttc
     8521 cggtctagga ccaggggaag aagtagtttc tcttggaatg cctatgagtg ttgaacttgg
     8581 accaggtctt atttcaacta tttatgatgg tattcaacgt ccacttgaaa aaatgtatga
     8641 tatttcaggt actaatataa ctaaaggtgt tgaagtatct tcacttgata gaacaactaa
     8701 gtgggaattt aaacctaaaa aatctgttgg tgataaggtt gtagcaggag atataattgg
     8761 tggcgttcaa gaaacagcag tagtagaatg taaaattatg gttccttatg gagttaaggg
     8821 tactataaaa gaaatatatt ctggtgaatt tactgttgaa gatacagttt gtgttattac
     8881 agatgaaaaa ggtaatgata tacctgtaac tatgatgcaa aaatggccag ttagaaaaga
     8941 aagaccatat agagaaaaga aaacaccaga ttcacttctt gttacaggtc aaagagttat
     9001 agatacattt ttcccaatca caaaaggtgg ggtagcagct ataccgggac cttttggaag
     9061 tggaaaaact gttacacagc atcagcttgc taaatgggca gatgcagata tagttgtata
     9121 tataggctgt ggcgagcgtg gaaatgaaat gacagatgtt cttcttgaat ttcctgaatt
     9181 aaaagaccca agaacaggcg agtcacttat gcaaagaact gtgcttatag caaatacatc
     9241 agatatgccg gttgctgcac gtgaagcttc tatatacact ggtattacaa tagctgaata
     9301 ttttagagat atgggatata gtgttgcact tatggcagac tctacatcaa gatgggctga
     9361 ggctcttaga gagatgagtg gtcgtttaga ggagatgcct ggtgaagaag gttatcctgc
     9421 atacttaggt tcacgtcttg ctcaattcta tgagagagca ggaaaggtaa attgtctagg
     9481 tatggatgaa agagaaggaa cacttacagc aattggtgca gtttctcctc ctggtggtga
     9541 tacatcagaa cctgtcagcc aagcaactct tcgtatagta aaagtattct ggggtcttga
     9601 tgcatcttta gcatataaac gtcatttccc agcaattaac tggttgacta gttattcact
     9661 ctatcaggaa aaagttgatg tatgggcaga taaaaatgta aatgataatt tctcagctca
     9721 aagagctcaa gctatgaaac ttttacaagt tgaatctgaa cttcaagaaa tagttcaatt
     9781 ggttggggtt gattcacttt cagatggaga tagattgata ctagaggtta ctcgttctat
     9841 aagagaagac ttccttcatc aaaactcttt ccatgaagtt gatacttata catcacttca
     9901 taaacaatat agaatgatgg gacttatatt aaaattctat aagtcagcac aagatgctat
     9961 caagcaaaat gttactataa acgatattat caagctttca gttcttgaaa agattggtcg
    10021 tgctaagtat gtagaagaag gcgatataga tgcagcttac gattcaattg aagatgaaat
    10081 agataacgag cttgcagacc ttattaaaaa gaggggggct gaagaactat gataaaggaa
    10141 tataagtcaa tacaagaggt agccggtcct cttatgttaa taaacggcgt agaaggtgta
    10201 aaatttgatg aacttggaga aattgaactt tcaaatggag aaattcgccg ttgcaaagtt
    10261 ctagaggtaa atggagatac tgcacttgtt cagcttttcg aaagctcaac aggtataaac
    10321 cttgctgaaa gtaaagttcg ttttttagga aagagtcttg actttggagt ttcaccagat
    10381 atattaggtc gtgtttttag tggaatgggt agacctattg atggaggtcc agaaattata
    10441 ccagataaaa gacttgatat aaatggagct ccaataaatc cagcagcacg tgattatccg
    10501 gccgagttta tacaaactgg agtctctgct atagatggac ttaatacatt ggttagaggg
    10561 caaaaacttc ctatattctc agggtctggt ttgccacatg ccaatcttgc agttcagatt
    10621 gcaagacagg caaaagtacg tggaactgat tctaagttcg ccgttgtatt tgctgcagtt
    10681 ggtataactt ttgaagatgc tgaatttttt ataagcgatt ttaaagcaac tggtgcaatc
    10741 gaccgttctg tagtgtttgt aaaccttgca aatgacccag ctgtagagcg tgtatctaca
    10801 cctagaatgg cacttacagc agcagaatac cttgctttcg agcaagatat gcatgtactt
    10861 gttataataa cggatattac aaactatgca gaggcgttgc gtgaagtttc agcagctaaa
    10921 aaagaagttc caggacgtcg tggatatcca ggatatcttt atactgacct tgctactatg
    10981 tatgaaagag caggaaaaat gaaagggcat gaagggtcta taactatgat tccaatactt
    11041 acaatgcctg aggatgataa gacacatcca attcctgacc ttacaggata cattacagaa
    11101 gggcaaatta tacttagccg tgaactttat aaaaaagata tacagcctcc tatagatgtt
    11161 ttaccatcac tttctcgtct aaaagataaa ggtattggta aaggtaagac tcgtgaagac
    11221 catgcagata caatgaacca gctttttgca gcttattcaa gaggtaagga tgcaaaagaa
    11281 cttatggcta tacttggtga atctgcactt tctgagattg atttaatgta tgctaagttt
    11341 gcagatgaat ttgagaaaga atatgtttca caaggattta gaactgatag aactattgag
    11401 cagacattag aaatcggatg gaagcttctt agtatgttac caaaaagtga acttaaacgt
    11461 atccgtgatg aatattttga aaaatatatg cctaaggagt gatatctgtg gctagattaa
    11521 atataaatcc aacacgtatg gaaatgacta gacttaagaa actccttaaa actgctacaa
    11581 gaggtcataa gcttcttaaa gataaacttg atgaacttat gaaacaattt cttgagattg
    11641 taagagaaaa taagcgattg cgtgaagaag cagaaaatgc tcttgatact gcttataaaa
    11701 actttataat cgcaagagca gttatgagtc aggagtatct gggttcagca cttatgatgc
    11761 caaagcaatc agtttcagta gatgtttcta cgagaaatat aatgagtgtt gatgttcctg
    11821 tgtttgactt taaaactgaa aacaatcaaa gtgatattta tccttatggt cttgctttta
    11881 catcaggtga attagattct gctatggaag ctttctcaga tgctatgcaa ccacttttaa
    11941 gacttgcaga aagtgaaaaa agtgcacagc ttcttgcaca agagatagaa aagacacgta
    12001 gacgtgttaa tgctcttgaa aatgtaatga taccaaatta cattgagact attaagtata
    12061 tagctatgaa gcttgaagaa aatgagagag caagtactac tagacttatg aaggttaagg
    12121 atatggtgct taaaaaagca cttgaagaaa agaaaaaaaa tgatttggtt gtataattaa
    12181 atacttataa aaataagtct aacattctac tttatagagg ttagacttat tttttatgtt
    12241 taaattaaaa tgtagatact ataaaaatta atatattgtt taaaaacaga ctgtatttaa
    12301 ctaaaaataa tatatttaac aaaaatttct aaaaattaac gaactgttaa tttacactta
    12361 actttatatt aacaaattag gtgtattata taaacataag caaaaacaat aaaaaacaaa
    12421 taaaaaaagg aaaaaacaca aagggggaag atatgaaaga atttatattt acttgtctgt
    12481 gcattgtagt gattacattt atgacaagtt gcatatcaac aacaccacaa actactcaag
    12541 gaaaaggaaa taataaactt acaatatatg cagcactaga ggaagagcag atagcttatt
    12601 atatggaagg ttttagagaa gagtatccca atatagatgt agagatagtt atggattctc
    12661 atggtgtaat agcttcaaaa ctattagcag aaaaagataa tccacaagca gatattatat
    12721 ggggattatc agcaatgaac atgatagagc taaaaaaaga tggtgcactt gaagcatata
    12781 agcctaaaaa ttatgataaa gttgataaaa aatttaaaga tactgaagat gatgtatctt
    12841 gggtaggaat gagtgtccca gagacagcaa tagtagttaa ttataaggaa cttaagaaac
    12901 taggtataga tattccaaaa agttatgaag atttaataaa accagaatat aaaggactta
    12961 taacaatgcc aaatccagca tcatctggaa cggggtatct tacagtatca gggcttattc
    13021 aaatgatggg tgaaaaagat gcttacaatt acatggataa attacataaa aatatagcca
    13081 catatacaca ctcgggttca aaacctgcga agttagctgg ctctggagag tatccaatag
    13141 gtattacact tggatataga gctacacaac aattatctag tggagaacct atagaggttg
    13201 tatttccaaa ggaaggttca ggctgggact tagaagcaaa tgccttagtt aaaaaagata
    13261 atataaaaaa agaagcaaag ctatttttag attgggcaat aagtgattca gctatggata
    13321 gatatgcaca tgaagtagca gttacttcca taaagacaga taatccagtt ccaaaaggtt
    13381 attctaaaca accatttgaa cagcttataa aagaagttga tttaaactct gctgccaaaa
    13441 acagaagtac agtacttaaa acttgggaat caaaatacgg ttcaaaatct gaaagtaagt
    13501 aaagtttaaa ttaataattt agatgaaaga attttagaag aaaaagtagg atataaaatt
    13561 taaaataaaa aaataactat ataaggagag ataagatatg agttacttaa aaataaataa
    13621 tgtgtttaaa agttatgacc aaaaaagggt tttaaataat attagtttgg atattgaaga
    13681 aggagaattt ctatgtttac tcggacc
//
LOCUS       DAYAOX010000101        13656 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.101, whole genome shotgun sequence.
ACCESSION   DAYAOX010000101 DAYAOX010000000
VERSION     DAYAOX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.101"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            167..751
                     /locus_tag="ACGI57_002223"
     CDS             167..751
                     /locus_tag="ACGI57_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HFG9717299.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(855..1634)
                     /locus_tag="ACGI57_002224"
     CDS             complement(855..1634)
                     /locus_tag="ACGI57_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFG9717300.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(1692..2948)
                     /locus_tag="ACGI57_002225"
     CDS             complement(1692..2948)
                     /locus_tag="ACGI57_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFG9717301.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGIAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            3206..4105
                     /locus_tag="ACGI57_002226"
     CDS             3206..4105
                     /locus_tag="ACGI57_002226"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFG9717302.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            4129..5577
                     /gene="ddlR"
                     /locus_tag="ACGI57_002227"
     CDS             4129..5577
                     /gene="ddlR"
                     /locus_tag="ACGI57_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HFG9717303.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            5660..6442
                     /locus_tag="ACGI57_002228"
     CDS             5660..6442
                     /locus_tag="ACGI57_002228"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFG9717304.1"
                     /translation="MSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSNPNKKGNIK
                     SQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGKSMPTDEPI
                     SFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEKIKLIDDYD
                     AVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDVTPKVSTVY
                     ADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            6468..8387
                     /locus_tag="ACGI57_002229"
     CDS             6468..8387
                     /locus_tag="ACGI57_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9717305.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGFIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(8629..8976)
                     /locus_tag="ACGI57_002230"
     CDS             complement(8629..8976)
                     /locus_tag="ACGI57_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HFG9717306.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(8963..10891)
                     /locus_tag="ACGI57_002231"
     CDS             complement(8963..10891)
                     /locus_tag="ACGI57_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFG9717307.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(11303..13492)
                     /locus_tag="ACGI57_002232"
     CDS             complement(11303..13492)
                     /locus_tag="ACGI57_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HFG9717308.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT         4970 a         2093 c         1750 g         4843 t
ORIGIN      
        1 ttacatattg taaatttggc catttactgt tattatgaat atggttgaaa ttatttaaat
       61 aaatactgtt gacttagttt catatattta ccattaaact tttatcataa atatcatatc
      121 ataataatat atttaataag tctaataaac tatacgaggt gtaaatatgt taatgaatac
      181 ccattttatt atggcaaaat caatacttga taatatagat gaaaataaaa cattctttat
      241 aagcgaaaaa aattttatat atggcaatat aaagcctgat gctttttcta aatataaatt
      301 aaaaaaacat tatttagatg agtcatttaa tatgattgtt tctaaaataa actatctatg
      361 cagtcttaat atagatagtc tttctaaata cttttcagta agtagactta gtcaggaact
      421 aggagtcata tgtcattttt tatgtgattt tttctgtgta gctcacagtg aacgttggga
      481 atttaagcat agcttaaata aacatgttac ttatgaaaga gaacttggag catttgcaaa
      541 agatgttgat ttatctaaaa ttaaatgtgg tactataaat agtagttttg agaagttttt
      601 tactaaactt tatagtgatt acaaaaagaa aactgattat atgaatgatt taaaattttc
      661 agcttatact tgtaatagtg tcataaatta tatacttgac tctattttat ctaatacaat
      721 aaaatcatat catatagcaa attgcggtta acttaagaat ttttaaactt atataaactt
      781 ataattgaat ataattaaaa tgactgctct atagaatata attttatact tctctaggac
      841 agtcattttt tatactaact atttatctcc attataacat ctatggcact ttttacacta
      901 tctactacat aatttgcact ttgctttaca tcttcccttg ccttagacat tgcaaaacta
      961 tatttaaatc cttctaacat gcaaatatca tttggagaat ccccaatagt agcaacttca
     1021 tctggactta catttaacgt ttttattaca tgctctatac ttcctctttt tgacacactt
     1081 ttaggaacta tatttaaagt atatgttcca gaaaaatata tttctaagac ttcactaaac
     1141 tcacttttta gataaagttc tattctggat aactcttcca aattttcaga acatacgcct
     1201 atatttccta taagattatc aaaactactt cttttttcaa aatctgggtc aactataaag
     1261 tcactagaat aatgtctaaa agcagtggta tcactaccca caattacatc acccttatat
     1321 ttaaaataaa gttgtgcatc acttgactca aatctatcaa tgactttttt tactaaatta
     1381 tcatctatcg ttcttttaaa aattacatta taatctttat catatataaa tgaaccattt
     1441 tcacatacat agtatccatt catatctaaa ctatctttga taatattatg tgcttgttta
     1501 aaaattctac ctgtagcaac attaaactgt actccctttt tttctatttc ttcaattgct
     1561 actatatctt cttttgttat ggttccattt tcatatagag ttccatctaa atcacaaaat
     1621 atatgtttta tcataaatta aaagcctcca aaaacaattt tatattttac taatacttta
     1681 atcaaaataa cttaaagctt ttctaattgt attctcaatt cattaagcaa atctctagta
     1741 gctcttaatc ttatctttct tctatcccca ttaaatatat ttttctttac tattgttttt
     1801 ccatttatat atattgcagt ataaaccagt cccacaggct tctctttggt tcctccacca
     1861 ggtccagcta ctccagttgt agatatacct atattagctt taagtccttt agcaatgccc
     1921 tcagccattt ctatagctgt ttgctcactt actgctccaa actcttccaa agtttctctt
     1981 tttactccta atcttttcat tttagaatca ttagaatatg ttacacaacc ttcaagaaat
     2041 acagaggata tacctggata gtttataaga gatgaagaaa ccaaacctcc agtacaagat
     2101 tcagccactg ctattgttag atttttttct acaagtagct tagaaacagc atcttctaaa
     2161 gcagtctccc cttcagatat ggaaatatct ccttcagtat atacatattt accaactcta
     2221 ctttttattt tttcttctac tggttttatt aatttaaaag cttctctttc attttcagcc
     2281 tttgcagtta ttcttattgt aacttctaat tcttttgcat ataaagcaac tgttggatta
     2341 gtctgctcat taattatatc gagaatttct aattctagat ttgactcacc tattccatat
     2401 aatcttaaag tcttagatac tagcatttca tttgtgaatt gttgtaagta aggtttgaca
     2461 ctctcattaa acatagcttt catttctctt ggaggtccag gcaatactat tataaattta
     2521 ccatcttttt ttaaaattgc ccctggagct gtaccattat tattttttag tactatagca
     2581 tcttttggaa agtacacttg tttcatatta ttttcggcca tttttttccc tgttttaaca
     2641 aagaaagatt ccaaaacctc tagtgaaggt ttatgtagtt ctaatttctg accaaagtat
     2701 tctgcagcag tttcttttgt catatcatca ccagttggcc caagaccacc tgtagtaatt
     2761 acaaaatcac ttctctttaa gctttcatca aaacattcta aaagtctttg tttgttatct
     2821 ccaacagtac tctgatggta tacttctatt cccaatgatg caagttcttt agctaaaaat
     2881 tgagaatttg tatttactat atctcccaat aatatttctg ttcctacact tattatttca
     2941 gctttcaaaa tttatcactc cttgatttat agccattaag actagtctta agttcataac
     3001 aataatttta gcaaaaataa taacataaat aaagttaaaa aatcatttaa acatatcttt
     3061 aaactagatt attattctaa aaccacacat aatataaact tcgtatatag tggtactttc
     3121 atcctcaact gttaattcat ataattaagt atgtaaataa aaagtttact ttaaaataat
     3181 aaaaattaaa ataggagtgt ataatatgaa aatagcagtt ataatgggtg gaatatcatc
     3241 agagagagaa gtttctttaa attcaggaaa agagatatat aataaccttg ataaaaataa
     3301 atatgaagtt gtaaaggtaa taatagatga taaaaaagat atatttacaa agataccaga
     3361 agatatagat tttgcaatac ttgctttaca tggaaaattt ggtgaagatg gatgtataca
     3421 atcaatactt gagactatgg atataccata ctctggatgt ggacctcttt gcagtgggat
     3481 gtgtatggat aaaaatataa ctaaaaaaat gcttagagat tcaaacctac ctactgctcc
     3541 atgggtttta gtaaaatcag ttgatgaaat agactatgat gaaatagata atataggtta
     3601 tccagtattt ataaaaccaa actcaggagg ttcaagtgta gccacattct ttattcattc
     3661 taaggatgaa gttgaagaag cagttagaaa aggtctggaa gttgatgaat ttgtaatgat
     3721 tgaaaaatat ataccaggtg gagaatatac ttcattcata ttaaatggag aagtgttccc
     3781 tacaatatct ataaaatctg attcaggatt ctttgattat gaagctaaat actcagttga
     3841 aaaaggtgca aaggaagaag ttgtttactt agatgaagaa cttcaaaaaa gagtaaacga
     3901 aatttctgag acttgttgga aaatttttaa ttgcaaagca tatgtaagag ttgatatgat
     3961 tataagtgaa ggtattccat atgttcttga actaaatact cttcctggaa tgactcaaac
     4021 tagtttaatt cctagaagtg ctgctgctag aggtattaag tacagtgaac tattagataa
     4081 acttatagaa tactctttaa attagatgaa ataatttgag gtggtaattt gatattttca
     4141 aatctaaaat taaatgataa tgaaccaatt tatatacaac taaaaaatta tataagtgat
     4201 atgatatcaa aaggactaat tccagacaat agtaagctcc cttctacaag ggagcttagt
     4261 cagcttttac aagttagccg aaattctgta gttttaactt atgaagaatt aaaatcagaa
     4321 ggattaatct actctatttc aggaaaaggt acatttgtta aatcaaagaa taagtcttca
     4381 aatactacat ggtcattaaa ctgggattgt ttagagaata catactctaa aaaagccaat
     4441 gaattggaca ttataaaaag tgaaattcct tggagctctg acctgatttc ctttaaaagt
     4501 atttctccag atggagattt attcgacatg gaagaattaa aaaaatcctt tttaaataga
     4561 atttcactag aaggacataa attactcaac tatggatatg cacaaggtta taagccactt
     4621 attgactatt tactagaata tatgacaaac aaaggtgctg atataagtaa taaagatata
     4681 ctaataacaa atggattcac agaaggcttt gatattatta tgtcatcttt tacccaagaa
     4741 aaagactaca taatatgtga aaatcctact cataatactg ctataaaaat tatgaaatct
     4801 cataacctca atatcttagg tgtagatata aatgaagatg gtttggactt caatatgcta
     4861 gaagacaatt tagttaagta taaaaataaa ataaagtttg catacattac accctcttat
     4921 cacaatccta caggaattgt aatgactcct gaaaataggt acaaattcta taatcttatg
     4981 aaaaaataca atatagcaat tatagaagat ggatttaatg aagaattact ctacaatagt
     5041 tctcatatat ttcctatctg ttccctagat aatatgaata atggggtcgt atatattggt
     5101 agtttttcaa aaattttatt tcctggaatg agaattggat ggatatttgc agataaaaaa
     5161 gtaatcaata agctagaaag tgtaaaaagg tgcagaaaca tacatgtatc ctttttagac
     5221 caaggaattt tatttgatta tttaagtaat ggaggttttg aaaaatacat taaaaaaata
     5281 cgtaaattct atggagacaa atttaacttt gcatataaat gtataaaaaa atatataaaa
     5341 acagaatata tcttgggtga tggtggtttg catatattta taaaactaaa aaatatagat
     5401 actagagttt tattagaaaa atgttatgaa aaaaatgtta tatttatgcc tggagactta
     5461 ttttttacag ataactctgg ttttgatact ttaaggctcg gattttctag gttgtctttt
     5521 gaagacattg aaattggaat aaaaataatt ggagaaacta taaatgaaat gttctaggtt
     5581 aactaaatat tgttagccta gtttattttt atgaggtata atatatatag tttatatgaa
     5641 tctaatactg ggggataata tgagtaaaaa gtcaaaacga agaaaaatta ataaattaaa
     5701 attaacgatt gtaataactg tattatttct tgctttatta tctatatatg agatactgtt
     5761 ctctaatccc aacaaaaaag gtaatataaa gagtcaagaa actatgcaaa ataaaaataa
     5821 tacaacagat gattctaaaa ataatcaaag ctctaccaaa aagcaggaag ataataatat
     5881 caaacctact ttttcatatt cttcaatacc agataatata aaaaataaaa tgattggtaa
     5941 atctatgcct actgatgaac ctataagctt tgatagtcta tcttatttaa aacttacata
     6001 ttatggattt gatgaaaaaa ctcatcaagg agaaatgata gtaaacagtg aactagctcc
     6061 agaagtagtg gatattttta aggaattata cgaaaaaaaa tatccaattg aaaaaattaa
     6121 gttaatagat gactatgatg ctgtagatga aaaatctatg tctgataata atacctcatc
     6181 attttgttat agaactatag ctggtacaaa tgtagtttca aatcatggta aaggacgtgc
     6241 tatagatata aacccattac agaatcctca agtttctgga aatgatgtta cacctaaagt
     6301 aagtacagtt tatgctgata ggtctagcac aaaatttgga atgataaaaa aaggtgatga
     6361 ttgttataat gcttttgtaa gcagaggttg gagttggggt ggctattgga aaaacccaga
     6421 ttatcaacat tttgaaaaat aatagattag aaataaaggt gaattatatg aatttaatga
     6481 cattggaaaa tataagtaaa agctattctg aaaaaaaatt actagaaaat atatctcttg
     6541 gaataaatga aggggaaaaa ataggattta ttggtgtaaa tggtactggt aaatctacct
     6601 tactcaaaat tattgcagga gttgaagaaa gtgagactgg taatataaca aaaactaatg
     6661 gtattaggat agaatatcta cctcaaaatc caacttacaa tgaaaattca actgtacttg
     6721 aacaagtatt taatggaact tctgaagaat taaagctact tggtgaatat caagaaatcc
     6781 tagaaaaact taattcaaac tttacagagg aattaaataa aaaattatta tcagtacacg
     6841 aaaaaataga tgccttaaat ctttgggatt tagaaagtga agctaagtca gtacttacaa
     6901 agcttggtat aaatgacttt aatcaaaaaa tagcagaact ttctggtggg caaagaaaga
     6961 gagtctctct ggcatcagcc cttattacac cttgtgagtt tttgatacta gatgagccta
     7021 caaaccatct agacaatgat actatagatt ggcttgagga atatcttaat tccagaaaag
     7081 gttcattgct tatgataact catgatagat actttttaga ccgtgttaca aatagaatta
     7141 ttgaactgga caaaggtaga ttattcagtt atgatgggaa ttattcaata tttttggaaa
     7201 agaaaatgga aagactttcg ctagaagcaa gtattgaaga taaaagacaa aatttaataa
     7261 gaacagagct tgcgtgggta agacgaggag ctcaggctcg tagcactaaa caaaaagctc
     7321 gtttacaaag atttgatgaa cttataaata gagattcttt taaacctgat gaaaatctcg
     7381 atatatctgt tgcatcaagt agacttggaa ataaaataat agaaatacac aatatatcta
     7441 aatcatttga agaaaatact gttatagata atttagaata cactttaact cgtacagata
     7501 gaattggtat aataggcaaa aatggtatgg gtaaatctac ccttataaat atacttaatg
     7561 gagaaataga gcctgatagt ggatatattt caattggaga aacagttaaa ataggttgtt
     7621 tttctcaaga cgactctcat atgaatattg atatgagggc tatagactat gtaaaagaag
     7681 ctagtgacta tatagaaact tcagatggta ctaggattac agcttctcaa atgtgtgaaa
     7741 gattcctttt taatggtact atgcaatata ctttgatagg taaattatct ggtggtgaaa
     7801 gaagaagact acatcttctt aaaatcctta tgacagctcc taatgtgctt cttttagatg
     7861 aacctacaaa tgatttagat attgaaacgc ttaaaatatt agaagaatac ctagacgaat
     7921 ttaaaggtgt agtagttaca gtatcacatg atagatattt cttagataga atttgtaata
     7981 aaatatttgc atatgaaggt aatggaaaaa ttcatatcta tacaggtaat tacagtgatt
     8041 atcttattta tagagagatt caaggaattg aatttgaaga atctaaaagg gaatcagtta
     8101 aaaaagatga ctctactacc ccaaaaaaag aaaaacctaa aaatgataaa aagcttaagt
     8161 tctcatataa tgaacaaagg gagtttgaaa atattgactc tgatatagaa aaactcgaat
     8221 ctaaaattgc aacacttgat gaaagtactg caaaattttc aactgatttt actaaactcc
     8281 aagaaatttt agatgagaaa tctaaactag aaaaagaact tgaatacaaa tatgaaagat
     8341 gggaatattt aaataatcta gctgatgaaa tagctaataa taagtaatat taaaataatc
     8401 ttaagtttaa taagttttgc tttaaatctg taatttataa atattttaaa ggaagtgtct
     8461 caaaatgaac tttttagttt tatgagacac tcttttttat gagaaatcaa atatattttc
     8521 atcatttcaa caatacaaaa gaggcttatc tctattttga gacagcctct ttcataatta
     8581 ccttctaatt tatatgctct aatatatcta taataattct tattatatct atatatccag
     8641 tctttcaata tagtgacaat taacttcaat atgatttaaa aatactaaca acctttcata
     8701 aagtggatga gattcttctc catatttagc ttctaggttc ttgcctattt ctctaacagt
     8761 cctttgacca tcaatttgta aaaaaacaaa actggaatat tcatctagtt ctattttttt
     8821 gtatttaggt attctagctt ttaactttct aaaaaacttt tgaattttat ggtcctgttt
     8881 ttcaagtata gtaacaacac gattttcagc tacttcatat tcaagtccat cagtaatttt
     8941 aaatacaata tctaaaacat cattactctt gctcattatt ttttacttta gataaaacaa
     9001 taggtaatgc tgtaagcaca actaaaacta ctaataaagc aattgccatt gaatttgttg
     9061 cagcaaatcc acttggtacc tttggagtta caactccagt tacttgtaat attataccta
     9121 ttaaaccaat gatagaacca ccagcaacaa gaccagaaga taaacttata ccattagaaa
     9181 ctttaacttc tttttctttt tcagttttag atactaattc aacaaataaa cgaattaatg
     9241 ctccaactaa tattatagaa gtagttgcaa ttggtaaata gaatcctata gctatagtca
     9301 ttattggtaa atttaatgca tatagaacta ttgccataaa tactccaaca ataatcataa
     9361 cccaaggtaa actacctgac ataataccag aagttaatgt tgacattaag tttgcttgtg
     9421 gtaatgcaaa ttgtacatta tcaccagttg ctctaagttg gtcagaaagt aataaaatta
     9481 ctcctacaac aactataaca ccaactatac ttgcaattgt aaagtaacgt tgcatttcgt
     9541 ttttgctacc accaataata taagttactt tttgagattg agtataacct ccagctatag
     9601 caatagcaac aactataaat gaaccaaata aaagtaatga tttattagct tctaaatcag
     9661 tccatcccat tataacaaac actaatgtta caataactag agaagctata gtcataccag
     9721 aaactggaag atttgatgtt ccgattgttc ctgttaaacg accagctact atgacaaata
     9781 ataaagataa taaaagagat atgatagctc caataattgc cataacaatg ttaccagaaa
     9841 ttaagaatgc tgctaaaaat ccaataacta ctccacctaa taaaagtatc atttgtatag
     9901 atgaaccttc ttctccttct ccagcacttg atttagcttt aagtgtttct tttatagaag
     9961 cgattatagt tggtataagt tttattgcac caataatacc tccacaaagc atcataccag
    10021 cacctatata ttttacataa ctactagata ttgctccaac atccatttgg ttaagaggca
    10081 ttgcaggatt attccatacc atagctccat ctttagccat atctgtaaag tatcctatta
    10141 aaggtgctat accaaagttt gataaaatac ttcctgcaaa cattgttaaa gaaacttcaa
    10201 gccctactat aaatccaatt cctaaaagta gaggatttac ttcaatatca aatttccatt
    10261 tgtagaattt gcttcctaca agactcatta cattgtttgc tacatttaag aatgaaccag
    10321 ttaatacagt aataaatcca cttattacaa atccaattcc catgtactta attgaatctc
    10381 caccagcctc tgaagcaaca agtgtctcag atatagccat tgattctggg tacataagtt
    10441 taccatgttc ttcaactatt agatagttgt gaactatagc agcaattcct ataccaaata
    10501 aaactccacc aactccaaca gctaagcctt ctaagaatgt aatttggctt ccaattaata
    10561 taactgctgg taatacgaaa ataaatccac ttgcaacaga ttctccacca cttgacatac
    10621 cttggattaa gtttttacca agaataccct ttgaacgagc aaatgctcca acaaaagcag
    10681 aacctattat agctcctgga atacctgcgg caactgtaag accggctttc attccagagt
    10741 aagttgtaga tgcggcaaaa atggcagcta aaataatacc gataatcagt acagctacat
    10801 ttccaccggt tctggactta tctgtgatat atggaacgta atcttttcca ctaacctcac
    10861 cataagcacc cttaggtaat tttttgttca taaaattttc accccttttc aatttgtata
    10921 tattataaca tatatacata taaaacttaa ctttttatta ttttacaaat agcgagtcaa
    10981 aactactaaa atttaaccct atattattaa tttctacttt tagatatttt tatgctaatt
    11041 ttctttttat ttttcaattc tactaaaaac tattaagata taaaataaaa aaatttttta
    11101 atgtaaaatt aattttgtaa aaaaaatgct ttaaaacacg gaaaagtata aaaaatacaa
    11161 taatcattct tataaataaa ttttcaccat agtaaatata tacagaaaat tcttgttttt
    11221 atgcaaattg attattttat aaaaataagt gtcataaaga aatgtctgaa atcactttat
    11281 gacacctatc ataataaaaa aattattctg gtaatgctct tttatttact tttccgattg
    11341 gtgtaactgg tatcttatct atcctcatta ttattgttgg aatcatatat ggaggtagat
    11401 gctcatttaa atattctcta atagttcttt catttatctt accactatct tcctcatcaa
    11461 ttacataata tgcacatata taaatatctc catcatcatt tgttctcgat atacaaacaa
    11521 cacttttaat ttctgtaata ttatttatta tattttcaat ctcatatagc tctatcctaa
    11581 actcatcaat ttttacttga ttgtccatcc ttccaatata atctaaattt ccatcatcta
    11641 accatcttac taaatcacca gttctataca ttaacttatt atgttcacaa gttttgtaag
    11701 gattctctac aaatacttgc tctgttagcc cttttttatt taaatatcct cttgatattt
    11761 gaatgcctga tagacatagc tctcctggtg ctccaattgg acatagtttg ttctttttat
    11821 ctaatacata tgcataagta ttaaatatag gttttcctat tggtattttt ccatattctc
    11881 tgtccacatg aaaagaagtc gatacaacag taaattcggt tggtccatac tcattatata
    11941 gttcataatt acgctttcta tatcctttta atctttctcc tccagtctgt acaactctaa
    12001 gtgaattatt attgcaattt tgtataaata accttgcaaa tgctgttggc aatgctagta
    12061 tagttatgtc attttcctca cagtattcat ttactgtata ttcatcaaat ttagcttctt
    12121 caggcataat atctattgat gcacctacaa gcaaataagg gaatagttgt ttcattacag
    12181 catcaaaact gaaacttaag tatataccac atatatcatt ttctgtagcg cttatagatt
    12241 ttacgctata ataacacata tttactaaac tcttatgttc tatagctatg gtctttggcc
    12301 taccagttga ccctgaagta gatatcgtat atgcacaatt tgatgatgtt attttttcag
    12361 ttaattcata tccaaactta aacatttctt catcatttat atcgagtact atggtattct
    12421 catttacaac cttacttttg agttcatctg tagttatcac tactttcgca ttactatctt
    12481 caagaaggta attcaatctt tcttctggat tacttgtgtc tattggaaaa aatgctcccc
    12541 ctgatttcat aatccctatt gctgtaataa ttatgttaat atctcttggt aacattacag
    12601 ctactatgtc ttcactttcc actcctatat gatttaaata acctgcaagt ttatcagtta
    12661 cttcatccaa ttctttatat gttaactttc tattttcatc cctagcagct atattactag
    12721 gtgttacctt aacttgctcc ctaaataaat caacataact tttatcatag ggtacttcat
    12781 attgattatt attaaagtct tccaataata ctttttcttc ttccttacat acaaattcta
    12841 aatcttctat taaaatatca ttatcttcta ttatttgctc tattatataa tcaaattgat
    12901 ttaaaattct tctaatagtt tctcttttat atatagatgg tttatacttt atttttattt
    12961 taagtgccct cttagtatat actattacag ataaatcata tccttttttt tctatgtcat
    13021 aatcatatga taaatcaaaa cttttagcta taaatattgt gtttataagc ttagtattta
    13081 gatgattttt attctctata tctgataaca aatagtatcc atactctaaa gacctgctcc
    13141 actgcctttt tagcttcctt acaacatcta taaatttttc actttgtcta actcttactc
    13201 ttattggaat tatattcatt gttttaccaa aaactacatc atcttcatat atatactttt
    13261 gaagtataag gccatatata gtttctatga tagagtctat gctaatattg ttcactttag
    13321 aatattgaat taacctttca tgtttctcaa tattcatctt aaaaacctca tctactactt
    13381 catcagaaca atctttataa aaaaaatcat ttggaatttt tgctatattc gtataatcat
    13441 ctaacaaatc catccaataa ttcatagctc gttctaaatt aacttcatac atatcaaaat
    13501 tcccccttac tactaagtct atagaaaaaa ttcttttgga attttatttt cacaatatat
    13561 aaatatatta ataatacata tgcaagctct aaaaacatga gcttgcataa aattacactt
    13621 attatgacta ctaaattcgt aatatttaca atgttt
//
LOCUS       DAYAOX010000102        13632 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.102, whole genome shotgun sequence.
ACCESSION   DAYAOX010000102 DAYAOX010000000
VERSION     DAYAOX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.102"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            385..1035
                     /locus_tag="ACGI57_002233"
     CDS             385..1035
                     /locus_tag="ACGI57_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFG9717309.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1249..1920
                     /locus_tag="ACGI57_002234"
     CDS             1249..1920
                     /locus_tag="ACGI57_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9717310.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            1917..3047
                     /locus_tag="ACGI57_002235"
     CDS             1917..3047
                     /locus_tag="ACGI57_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9717311.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKLEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3348..3470
                     /locus_tag="ACGI57_002236"
     CDS             3348..3470
                     /locus_tag="ACGI57_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717312.1"
                     /translation="MKWYTWLTIILIILLSIPLKIKFIKWWDRRQKNDKGGYDR"
     gene            3467..4312
                     /locus_tag="ACGI57_002237"
     CDS             3467..4312
                     /locus_tag="ACGI57_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717313.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4312..5340
                     /locus_tag="ACGI57_002238"
     CDS             4312..5340
                     /locus_tag="ACGI57_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717314.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDDYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5343..6356
                     /locus_tag="ACGI57_002239"
     CDS             5343..6356
                     /locus_tag="ACGI57_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717315.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLYYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLF
                     FSR"
     gene            complement(6873..8123)
                     /gene="cme"
                     /locus_tag="ACGI57_002240"
     CDS             complement(6873..8123)
                     /gene="cme"
                     /locus_tag="ACGI57_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HFG9717316.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8209..8955)
                     /locus_tag="ACGI57_002241"
     CDS             complement(8209..8955)
                     /locus_tag="ACGI57_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9717317.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9070..10092)
                     /locus_tag="ACGI57_002242"
     CDS             complement(9070..10092)
                     /locus_tag="ACGI57_002242"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFG9717318.1"
                     /translation="MNKNNCLDLIFEKVNPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTYSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGAGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10092..12512)
                     /locus_tag="ACGI57_002243"
     CDS             complement(10092..12512)
                     /locus_tag="ACGI57_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFG9717319.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSYNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEVRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12598..13518)
                     /locus_tag="ACGI57_002244"
     CDS             complement(12598..13518)
                     /locus_tag="ACGI57_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9717320.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         4784 a         1790 c         2156 g         4902 t
ORIGIN      
        1 tctatactat aattatttaa aactaaatat aaatgttgga attatttagg acttgaaatt
       61 gtctaaaaat aaatataagt gatgaagagg tgatgtgatg agattagata tggatagtat
      121 gttatatgta ctaatttata tgacattatt ggtatctatc attggaagta tattttattt
      181 ttgtaaagac ctcttaaatc ataaaattaa gtatgtttta ataagagaca tatttgtaaa
      241 tagaattaca tattctgaag aacggttgcc taaacagtat atagtttttc agaaatatga
      301 tatttggagg tattgtagtt tgttttaatt tgaaatgaat ctattataaa ttctaaagtt
      361 taaaaataaa aaggagagaa taaaatggat ttcatttcaa gtatattgca aggattatta
      421 agtatattat ttaattttac aggtgatttt ggaatttcta ttgtacttat aactttaata
      481 gttaaattag tacttatgcc actatcatta aaacaaagat tttctgtaaa aaaacagcaa
      541 gaattagctg aaaaaatgga acatataaaa gaaaagtata agaatgatac taaagaattg
      601 gaaaagcaaa tacaagtaag ttctgtagaa agtatgaaga gtatgctggg atgtcttata
      661 gttttggttc aaatgccagt agtatatgct ttatatcatg tatgtttaga tatgacaaag
      721 gaatttacaa caattataat tccatggatt gctaatttag gtacatttga taatttgttt
      781 atattacctt gtatatatac attaattatg ttagcttcaa atttagtgaa ttatatacca
      841 tatttaaaaa taaattctca agtaaagttt aacaaacaaa tggctgtggt gactaccata
      901 acaagtctta tactaactgt tagaactcca gtagccatag gtctgtactt tataacaagt
      961 gcaatttatt cattaattga agatatttgt tttagaatat attttaatcg aaaaaataga
     1021 atagtgacaa aataatataa ttattattag aaatatttta gcctgctaac atagaaaaag
     1081 ttagtaggct atttttatct atgatatcaa ttccattaag aatagtctaa aaaatactat
     1141 caaaatgcta taaaaaatac tatcaaaata ctataaaaaa atagttgaaa aataaataaa
     1201 ttaatataaa ctaaaataaa gcctacaaaa aagaggtgat aaatagaaat ggctaaaata
     1261 ctaattgtag aagatgaaaa aaggatgcaa agtattattg tagaatatat gcaaaaggga
     1321 ggatatactt gtataactgc tgatgatgga gtcgaggcac ttacaatatt aaagagcaac
     1381 aatatagatt taatgatatt ggatattatg atgccgtatt tagatggatt ttcagtgtgt
     1441 agagtttcac gtgaaatgac taatatacct attattatat tgacagctaa aggtgaagag
     1501 gaggacaaat taaagggtta tgaatatggc gcagatgatt atataacaaa accatttagc
     1561 cccaaagtac ttttagcaaa agttaatgcc ttgttacgta gatacactac tgatattccc
     1621 aaaaattcat tgagtttagg aaagattttt attatggtag catcaaggca agtgtatgta
     1681 gaagataaat tgattgattt aacatataaa gaatttgaat tattgcgttt atttatggaa
     1741 aaccctaatc aagttttttc cagagagaaa cttttaaatt gtatatgggg atatgatttt
     1801 gaaggaaaca cacgaactgt agatacacat ataaaaacct tacgcaaaaa gcttggtagt
     1861 gaaggacacc atatagttac attaattcgt tcaggatata agtttgaggt taaggaatga
     1921 atagtttaag aaagattata gaaaaactga aaaatgtaga tatttcaaat tggattaaac
     1981 aaaattttgg actatattca gttagaaaaa aagtgttttt actttctaaa ttatcaggag
     2041 cgttaattat attattttat ctggttgttg aagaattttc gataaactct aaattagaat
     2101 tttggatttg gtttataata ctggttatat ttattatgag tattgatttc ttattgggaa
     2161 aatttatttc agaaccaatc acatctataa ataaatctgc aaaaagtatg tcccaacttg
     2221 atttttctaa tccatgtact gtaaatacta atgatgaatt tggagaactt tctaggagcc
     2281 tgaatacaat gtctacaaat ctacaacagg cattatcaga cctagaaagt gcaaatattc
     2341 aacttgaaaa ggatgtaaat aaggaaagaa tgctattgga acaaagaaag gagttggtag
     2401 atacaatttc acatgaaatg aagactccac ttggcattat acgtgcgtat acagaggggc
     2461 ttatggatga agttgatgaa gaaaaaaaga aaaattatat gaatgttata atagaagaaa
     2521 ctgatagaat gaacaatatg attgtatcat tattagactt atctgcattg gaagcaggag
     2581 tttctaaact taacccagag cgttttgatt ttatagagtt tgttgaaact gtggcaggtc
     2641 gtcttttaat tgatatacca gatgccaatt tttattttac atatgatttg ccagagtatg
     2701 aagtttttgt agtggcagat aaaatgagaa tggaacaagt agtagagaat ctagttatta
     2761 atgcaaagaa gcatgttgta tataatggta atttagattt atcgattaca tgtgaaaatg
     2821 gcttgctttt atttgaaatt tataatgatg gtaggtttat agagcaagat gagatttcaa
     2881 agatttggtc taagttttat cgtagtgttc aatctcagcg tacaggaggt tcaggtctag
     2941 ggcttgcaat tgtttcacaa atacttacaa tgcaagggtt agaatatgga gtgaataatc
     3001 gtgatagagg agtagagttt tattttatga ttccaattga tgaataattt agtattaatt
     3061 tataaattat aagtatctga tgtaaaatat ttttctttgt atatgtaagc atttaaagag
     3121 aataaaataa aaaaacttaa tgaaaaaagc ttaatgaaaa agtctaataa aaagcttaat
     3181 gaaaagtctt gtttttctga tgaaaacaag attttttatt aagcttttta ttatttcact
     3241 tcgatttcac aagagagaca ctctgatttc accaaataga aataaaatag gttaaataaa
     3301 ttaaaaaata gaagcgtaat taaataaggt tacaaggggg agtacttatg aaatggtata
     3361 catggttgac tattatattg ataatattgc tttctattcc gcttaaaatt aaatttataa
     3421 aatggtggga tagacgacaa aaaaatgata aaggaggtta tgatagatga taaaagtagg
     3481 agagttaaaa ttttcttatg gaaaagataa acagatattg catggactta attttgaagt
     3541 aaaagaagga gaaatctttg gattccttgg acctaatggt tcaggaaaat ccacaacaca
     3601 gaaaatctta aatggtgttt taaaaggtta caatggtcac gtatcccttt ttggtaagga
     3661 agttaaagca tacacagaat ctttatatca aaagattggt gttttatttg aatttcctta
     3721 tttatatacc aatttaagtg caattgataa tttagaatat ttttcgtctt tttatcctaa
     3781 aaatcaaagg agagatatat atgaactctt agatttgtta gagtttaaga aagagtttat
     3841 aaataagcct gtatcatcct actcaaaagg tatgaagcaa cgtattagta tggcaagagc
     3901 attggtaagt aatccaaagt tgttattttt agatgagcct actagtggac ttgacccatc
     3961 tggagctgta ctatttcgta agattattga agaagaacga aaaaaaggaa caactatctt
     4021 tttgactact cataatatgc tagatgcaga tttaatgtgt aacaatgtgg cttttattgc
     4081 agatggaaag attatggtaa tagataaacc caaaaattta aaaatgaaaa atagcaataa
     4141 taaagttgaa gtggaatttg tatataatgg aaatcgagat attgaaattg tagatataca
     4201 agagttagaa tctggaataa cttttaaata tgatgaaatc ttaagtgtac attcaaaaga
     4261 acctacacta gaagaagttt ttattaaata cacaggaagg atgttagtgt aatgtactct
     4321 aaatttttta ccttagtaaa aaaagatttt tggatgatga tttatggtaa attttttgtg
     4381 ctgacacttg gctctctcat actttattcg tgttatatta attttggtta tgtaaagttt
     4441 atggatgaag atagttatca caataatgta tatctatata atccagagaa tataagcatg
     4501 agtgcatctc cattaattaa tcaagtttct tctataggtg aattaaaagc aaaacttgat
     4561 gaagatacaa gtgcagtttg tattgatatg agttccagta agccgaaagt aattttacat
     4621 gaaagtatta aaggagttga cgattataga gctgactatg cattgtcatt attatcatca
     4681 gaaaaagacc acaatagcaa aatatcagac aacaatgcaa taattcaaat taatgataag
     4741 gatacatctg agataaaaca aagaaaagac atgacttgtg agattttatt ctttgaaatt
     4801 gtagctattg gatttttagg gattgcctct ttattattta aagaaaagca gatgggagta
     4861 atgcgtatta ctggtatttt acctgtaaaa agaagtcttt ttatattatc aaagatattt
     4921 gcttttttga tatgtgacct atgttttaca gttcttttag tagtatttaa tattggattt
     4981 tttgaaggaa taaaaatact tccacaagta ttagttcaag ctagtatttt atcaattatt
     5041 atgtctttga ttggaatagg atgctcaatg ttattgagag attttaagca attttctctt
     5101 atatatcttg tggttgctgt attcataact acacctatat ttctatctgc aaatacatct
     5161 tttgaaatag attggattaa atatcatccc ttctatcatg tttatagtca gcttaaaaat
     5221 gcgtttttca tgaatttttc aaaagaatta gtttattttg tagtgtgttc tttgacaata
     5281 atagttctat ttttacttgt aaaatatatt tttaataaag agttggttag ggagggataa
     5341 tcttgatagg attacgttac cagttaaaga gtataaaaaa agataaaatg tgtataattt
     5401 ccttcttttt accagttgtt atggcgattt taataaattt tatgggaaca attgatatat
     5461 cttcattagg agagatacgt tttggtatag tgaaggatac acttcctgta caaacagagc
     5521 agtggttgaa gaagtatggt atggtaagtg tatatgacac aaaaatgcat ttaatacgtg
     5581 aaattaagga tattaaaaca gacacaattg gagttgttag agatggtaaa gggataaaaa
     5641 ctgtgcttta tggaaatgag attaatgcta taaaaaatgt tggaaagaca ttgccagaga
     5701 tgtttagact aaagggtgat ttaaaagatg ttgaggttac aatattgcct aaagatagta
     5761 ttttaaaaga gtttcaaaat gtttttattg ccatgatttt aattactgct atgtttatgg
     5821 gatgcacctt taatgcaatg aatattatat ctgaaaaaga agatggaata tcatttataa
     5881 atgaagtttt gccacagagt agaagtcagt atattataca aaaagtattt ttgggtcttt
     5941 tatgtgggtg tttatcttca atgataactg tatttatatg tttggatatt tcaatattag
     6001 atatgataat gatgttgtta ctcgttgtat tttcagcatt tatttcatca cttataggtt
     6061 tatttattgg aaagttgtca gaaggtctta tggtaggaat tgtttatatt aaaatagtaa
     6121 tgattttatt tataggtact cctttgttaa tatatcttct aggggttaat accagtggta
     6181 tagggaattt atactatata atgccttcag ttgctacatt taatggtatt atggagatta
     6241 ttaatggtaa tgtcattatt atcaaagata ttgtaatttt ggctgtacat tgtgtagtat
     6301 ggtttatgat atatattatt ttagataaaa aaagaaagct attttttagt agataactca
     6361 gtataattat aattgggaca atttaatagg tgatttaata tactttgtat agtgtaattt
     6421 atgtagtgca atttgtatac tttaatttat gtagtgtaat agttagtttt attttataat
     6481 tattcaatat aatttagtaa gaagttcagt acaattagag ctacttagtg gaaaacttaa
     6541 taggtactta aactatataa atactttatt ttcctccatt tgtactataa atttaattta
     6601 tgatataaat ggaggtatta ttatatgatt tgataaattt catcattata acctacaagt
     6661 aaaggtatta ttatataaag ttaataaaga tagtatcaaa atattatttg ctataaagag
     6721 aggggctcaa aataaagata aacctatttt tcaatgttga aatgataaaa tatatttgat
     6781 ttcatataaa aaattttctt cataaattaa aaagtccatt ttaagccccc tcatttttaa
     6841 ccagataaat cgagttaaac tatttaaata tattactcgg attcatttga cttagatatt
     6901 ccttgcttgt aaaattgttt tgaatagggg atagccaaag caaaaaataa aagtataatc
     6961 cctgctatga ttattaaatt attaatagaa attacatctg ccattggtcc gaaaatcatc
     7021 ataccaagag gaataaaacc agtatatata ataataggta agctaagaac tcgacctagc
     7081 atttgtggtg ctacatgctc ttgcaacata gtcatagctg atgcttgaat gattggaatt
     7141 gaaaaattcg taaaaaacat aaatataaaa aattgccaag cttgtgttac aagacctaga
     7201 ataattgaaa aaatagcata ggataatatt gctagaaaaa aggtcttatt tctattttta
     7261 aaaccaccaa aaactcctat aatcaatcca cctaaaacca tacctaaaaa tccaatcatt
     7321 tcaataatag acaggtatat ataacccttg ccaaatgttc gttcaataag aagtcctata
     7381 agaaatcctg atggaacaca aagaagcatg aatgctccat aagtaaaaag cacttttcta
     7441 atgaacttat ctgaaaaaca gtactttatc cctgatacca tatctgcaaa aaatgagact
     7501 ttttcagttt gttttggtat atcatgttta ggaattttga tgaatatcaa taatacaata
     7561 gcaataattg cagtaaatac atcaattaaa agaacttgat ttattttatt accaaatcca
     7621 agtattgcag cagctacaat aggaactata aattgtacta tagattgtag gctactattt
     7681 ataccattaa aacgcattaa atgttcttct ggtacaagtt gaggaatcat tgcgtttaca
     7741 gcaggtgttt gtacaccact tcctagtgaa cgtataacag atattactga tatggctatt
     7801 aattcacttg ttgtgccatt tgatgacatc ataaatagaa ttagaactaa tgtagatata
     7861 gcaattgttg aatctgatac aattatgagt acctttcgat tgtagtagtc tgcccataca
     7921 ccagcaaaaa aagaaattat catttgaggt ataaaggagc atacagttaa aactgttacc
     7981 catacacctg atgatgtctg aagtgtgaca taccatatca ttgccatttg tacaagtgat
     8041 gagccaaata aggaaatata ttgacttgct aaaaaaagaa tgactttttt cttccatgta
     8101 gaattagtat ttttgttttc catcctattc ctcctttgat ttgtgtgttg aatttttctt
     8161 gctcttaagg ataacacaca aatcaaagtc tggagttaga gtggagggct aggaaaattg
     8221 ttttttcata atttgaagat gagatttcat ggtttttgca ttatgttcag cttctttcaa
     8281 tgaagttatc agtttgtcac acactgagat aatttcgtca tcatcttttg gagtgtcaag
     8341 gacagtttta atattagtat taggattaga agataaagca tttaacattc gtaaaatagc
     8401 tgaaagagaa tagtttgcac aacgaagtgc gcttataatt ttaagtcgtt ttatatcttc
     8461 atcagtatat acacgataac cattttgttt tctttttact tttaacaaac cattcatttc
     8521 ccaatttctt aaagtatcta ttgtaacgtt taagtgttct gcagtttctt ttctagttaa
     8581 aaagagttta tttgtttctg gagtatttcc atttaaaatc tgtgcaacaa tagtaagtgc
     8641 ttcctcggca ttagattttt ctatttctaa ttgttgcaag tattgattta caagactaat
     8701 agccttatca aagtccctat tcgctgatgt tcttacaatc tcaagggcat cttttcttaa
     8761 tcccctttgt accacttcaa ttttcaaggc tgtacgagca aatctaaatt gttctatatg
     8821 aaaatcatta aagatacgat aaccattggt attacgctta ggtttaggta ttaagtttag
     8881 ttcttcatat aaacgtactg tattaggatg tacaccaatt aaatctgcta cctctgatgt
     8941 cgtgtaagtg tccattagta ttcaactcct tttagatata tagtattagt cataataaca
     9001 ccaaacaaaa gtctggtgtt ataatggagg gtttggttat ctaagagtaa ttaatttatg
     9061 aaagagtaat taatctatta tagaagtaag tatatatttt tcaataaact cccatctatc
     9121 acgaggttta aacactattt tagatggtat ggctattact aagtcatatt gtggaataca
     9181 acaaatagca tttcctccag ctcccattgc tgaataccac aaaacaccat cttttttaca
     9241 tagccaccac agataaccgt attcattact attcatttta actgattctt caatccacga
     9301 tttagatata atacgtttat tattccaaat accatcattt aaatacagaa aaccaaatcg
     9361 caacatatct ctagcactca atgtaagtcc ccagccacct atagaataac cctgtctatc
     9421 ttttgcccaa cccttaagat tttttccaaa gaaatcttca aaaccaaaat ctttcatatc
     9481 ataatctggt ataactttca taccaatagg tttaaataag tattcattag caaattcacg
     9541 agcagatttt ccagtagttt ttgttataat agctgaaagt aggtgcgttc cagctgaaga
     9601 atatttgaaa gtaccaattt cgcctttttg accaagttga ttcaaggtat attttatcca
     9661 atctttttgt ttgcaaagtc tttctaaaga ctcagtcata tttttaaaag ggtatggtgc
     9721 agtcattgta agtaggtgac gtatagttac tgctttttta catatttcag aaggactata
     9781 agtatattca gggaaaaaat caacaacctt ttggtcaaca tcttttatat atcctttatc
     9841 tattgcaata cctattaagg ctgatataat actttttgta actgatgcaa catgaaaagt
     9901 atcatcagca ttatatccat tatagtactt ttcaaagaca acttttcctt ttcttgcaat
     9961 aattattcca tttatattac tatattgtga gattattaaa ggttcaagtt gtgagagttt
    10021 ttctgaactc atgccaacac ttgaaggatt tactttttca aatattaaat ctaagcaatt
    10081 gtttttattc attaaagata cctccctgtt atagattttg gattttcttt tagagttttt
    10141 ggagaacctt ctgcaattat ataacctcct ttgtcaccac cttctggacc aagctcaaca
    10201 atccagtcac aaacatttaa tacatctata tcatgttcaa caacaattac agaatttcca
    10261 ttttcaacca attcatctaa taattgaatc aacttagaag tatcgtatag acttagacca
    10321 gtagttggct catccaatac ataaaggata ttaccatttc gttttttacc aatttctttt
    10381 gcaagtttga ttctttggga ttcaccacca cttaaagttg gtgtaggttg gcctaatttt
    10441 atatacccca tacctatttg ttctaataca tttaagtgtg aaacaatatt tggaatatcc
    10501 ttgaagaaat gtacagattc agaaacactc atatttaaaa tatcatgtat atttttattt
    10561 ttatatttaa tagataaaga ttcatcatta aatctttttc cctgacattc actgcatata
    10621 tggtctatac ttagattatt tcccaaccag accttttcat atccactacc actacattta
    10681 ggacaagccc ctttggagtt aaatgagaaa tgaccagctg taaatcctcg ttctaacgat
    10741 tctggctgac ctgcaaatag tgtacgtatc ttatcccata taccaatata agaagcagga
    10801 gttgagttca tatttttgcc aataggagat tgagatatct ctgaatatcc agaaatatgt
    10861 ttagtaccaa tcagtctatc tgcaatagtt tcaacaactg cataatcgtc ttcctcacct
    10921 atttcactct ctgaagaatt tatttgatta ttgatagtat ggttattaaa ttggcttcta
    10981 agaagaggaa gtagggtgtc tgatataagt gaactcttgc cactacctga ttttccagat
    11041 attccaacca tagcacccaa aggtaatgat acagttacat ttttaagatt atttgtattt
    11101 gcattttgaa tagttataca aggtatttta tcagtatgtg taatgttttt attgcttgtt
    11161 ctattaggca ttggatattt tccagataga tactgtccag tgagtgattt gtcggattga
    11221 agtagacctt ctaaatctcc ttgatatact aattgaccac cttcaacacc agcttttgga
    11281 ccaatatcaa taatatgttc tgccatttta attgtgtttt tatcatgctc aactactatg
    11341 accgtattgc ctaaatcttt aagtttcttt attgatttta agatgtctat tttttctgat
    11401 tcatgcaatc cagctgttgg ttcatcaagc acataaatta atgagtccat ctctgaatcg
    11461 agatgtgaat ttaaaaatag tctttgcatt tccccaccac tcaaagttga catttctcgg
    11521 tataaggata gatgacctaa tcgtgattct attaaatgtt gagttttgga taaaatatca
    11581 tttacaagat ttgtaccaaa tgtgtttaaa ttaccttgat ttattaagag tttaaggaaa
    11641 tcatcaattt ctaaaatcgt catttttcca agttctccaa tatgttttcc gtttaaaagt
    11701 actctacgtg cttcttcacc aattttaaaa ccattgcaat ctgaacaggc agtcatagta
    11761 taaactccat ttaaatcttc gccccttttg taacgattgt gaaatacctt tgataaagag
    11821 gaactttttt taccattttc ataatgacca tagagtactt catttcttac ttcttcaggt
    11881 agttgtaaga attgtatgtc taaataatca ttgaattttt tagaaaatac tctcaaatat
    11941 ccaggtgtaa tctttgctct atctaagact tgttttaggg tatcatgctt atcaaggact
    12001 aatttttcaa tattaatctc aaaatatgca ccatgtccag agcacttcat acacattcca
    12061 tctgaagagt tgtatgagaa ataacttggt cttaatcgtt cttcctcata tccacaatta
    12121 ttgcaaatta gattatagct tacaggagta tcacacatag tacacattat ttgaccttcc
    12181 actgaataca atagtgtaag catgttgaga atattggttc ttgtgcctac tgttgaacga
    12241 ggattgctct ggcgaataat attttgttga acagctatag tgggagctaa accttgaata
    12301 ttatcaaact tatcttcact atcaatacct gcaagtatac ctaaagattg cagatattgt
    12361 ttacgccctt cctcaaatac aatgtcaaac ataaggctag acttacctga gccactgaca
    12421 cctgttgcaa caacaagctt atctttgggg atagaaacat ctatattttt aagattgtgt
    12481 atctttgctc cattaatttt tatttctttc attggttcac ctcattttat caattttatc
    12541 aaaccgtaaa atagggatat attaatcctt aatacaagaa tagattaggt gaggttatta
    12601 aaaaggttct ataggtaaat atatatcgac caagtgtcta ttttgagggt gattgtgtgg
    12661 gttgttaaga tatacttcaa aagggaagga ggcacgtagc ttataattac catttgatag
    12721 ccattcatta tacataaaat cccacgcatc actatattca gattttgaaa tatcaaaatg
    12781 tcccacaaga tattttccag aaggtattat catattgcca agattgtcat tctctttgac
    12841 aacaatgtta tctggaatgg tcatgcaaat actagtcctt aattgatgct cctgtgtaaa
    12901 ttgtggatta tcatgataaa tggctaatat ttgtgtgttt tcaagtccaa gtagattctg
    12961 ctcacttgca tatgtgaata accgttctaa aagttttggg aaagtactgg ttagattacc
    13021 atatgaacca gtatacctag tgtaaatagt attcatatta tcaagttcta gaatttcaat
    13081 ttcaccttta acctctctag tattcttaca tttaggtttt gatatgcttc tattgtactg
    13141 agaaatttta tatggttctt tgcaattctt gctataattg tttctatatt ttatagggct
    13201 tattttatag taatttttaa atgctcttga gaaaactgct gaatcagtaa agccaaagta
    13261 gtgtgcaata tcagtaacag tcatgtcagt tctatgaata agaaaagcag ttgcattttc
    13321 tagttttagc ctatttacat attgtgccaa agtttctttt tctattgctt tgaagattct
    13381 atgaaaatga aattttgaaa aacctgctat attagaaagt tcatcaagag aaagaggttc
    13441 atgtaaatgt tcttctatat agtcttgcac tttgcaaata cgatttagat attctttttt
    13501 acttttagca tttgtcatat atagccctcc ttattgtgta aaatgtgtat caattaattt
    13561 atagcaatat agtaataaaa ttttatgttt ttgtaattta tgataaataa gatagcgttt
    13621 aattttttat aa
//
LOCUS       DAYAOX010000103        13528 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.103, whole genome shotgun sequence.
ACCESSION   DAYAOX010000103 DAYAOX010000000
VERSION     DAYAOX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.103"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            299..2539
                     /locus_tag="ACGI57_002245"
     CDS             299..2539
                     /locus_tag="ACGI57_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9717321.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            2953..4488
                     /locus_tag="ACGI57_002246"
     CDS             2953..4488
                     /locus_tag="ACGI57_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9717322.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            4504..5265
                     /locus_tag="ACGI57_002247"
     CDS             4504..5265
                     /locus_tag="ACGI57_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9717323.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            5603..6472
                     /locus_tag="ACGI57_002248"
     CDS             5603..6472
                     /locus_tag="ACGI57_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9717324.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            6801..7052
                     /locus_tag="ACGI57_002249"
     CDS             6801..7052
                     /locus_tag="ACGI57_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HFG9717325.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            7164..7943
                     /locus_tag="ACGI57_002250"
     CDS             7164..7943
                     /locus_tag="ACGI57_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9717326.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFINYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(8078..8728)
                     /locus_tag="ACGI57_002251"
     CDS             complement(8078..8728)
                     /locus_tag="ACGI57_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717327.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(8725..9228)
                     /locus_tag="ACGI57_002252"
     CDS             complement(8725..9228)
                     /locus_tag="ACGI57_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9717328.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(9277..9792)
                     /locus_tag="ACGI57_002253"
     CDS             complement(9277..9792)
                     /locus_tag="ACGI57_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9717329.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            10072..10599
                     /locus_tag="ACGI57_002254"
     CDS             10072..10599
                     /locus_tag="ACGI57_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9717330.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            10583..11986
                     /locus_tag="ACGI57_002255"
     CDS             10583..11986
                     /locus_tag="ACGI57_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9717331.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(12369..12680)
                     /locus_tag="ACGI57_002256"
     CDS             complement(12369..12680)
                     /locus_tag="ACGI57_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717332.1"
                     /translation="MKKIIKSFTSWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(12736..13329)
                     /locus_tag="ACGI57_002257"
     CDS             complement(12736..13329)
                     /locus_tag="ACGI57_002257"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9717333.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT         5048 a         1550 c         2267 g         4663 t
ORIGIN      
        1 gtaactaata ccattttgag attattaact taagaatttt atataattta acactcatat
       61 ataatgatga tatttaggta gaatagatgt aattacgctt aaataaaata actgtatttt
      121 ttataaaaat ccaaacccta cactatacta ccagataaaa tctacatgct ataatttgac
      181 ctgtgtttat aaaaaaagga gtaatctaat aaaatagtat ttcacttatg tataactaac
      241 aactattatt acgattttct ctaaataatt taaagaaaaa taatgcagga ggtaaaatat
      301 gaacaatgaa tttataattt taaaaggttg caaagagaac aatttgaaca atatttcctt
      361 aaaaatacca aaaagaaaaa taaccatatt cacaggggtg tcgggttctg gaaagtcatc
      421 tattgtattt gagacaattg caaaagaatc acaacgccaa ctaaatgaaa ggtttagtac
      481 atttgtaaga agttttttac ctaagtatgg agaggttaaa gctgattgta tagaaaattt
      541 gtcaacacca attatcatag accaaagtag acttggtgga aattcaaggt caactctagg
      601 tactattaca gatgtaaatt cctttttaag agctttatat tcaaggtttg gtagtgagta
      661 tataggtaat gccaatatgt tttcttttaa taatattgat ggtatgtgcc cacactgtca
      721 tgggcttgga aaaaagctag ttcctaatat gaaccaaatt cttgatatga ataaatcatt
      781 aaatgaaggt gctatattgc tttcagggtt tggagtgggt tcttggcatt ggaatatatt
      841 cgctgaatca ggattttttg atattaataa gaggatatgt gattattcag aagtagaatt
      901 ggaaaagttt ttgcatggta aagcagaaaa gattaagata gaaaatgctg gtcaaactaa
      961 catgacatat gagggattaa tggttaaatt taatcgccta tacttaagta gagagggaga
     1021 aatatctgaa gctactaaga aaaaattgag taaacttctt atagaagata gatgtcctat
     1081 atgtgatgga agaagactta atgaaagagt atataggtct ttaattaatg gttataatat
     1141 agcagattta actagtatgc aaattgatga attggctgaa gttattaaaa gtatagatga
     1201 gccagaagca caacctttaa ttaaaggaat aatagaaaag ttaaacagta tcatagatat
     1261 aggtcttgga tatttaacac tagatagaga gacatcttct ctatcaggag gggaatctca
     1321 gcgtataaag atggttaagt atttaaatag taatcttgtt gatttaatgt atatttttga
     1381 tgaaccaagt gtaggacttc atccaaaaga tgtgtataaa ttaaataatc ttttaaaaaa
     1441 attgcgtgat aaagggaata caattatagt tgttgaacat gacccagatg ttattaagat
     1501 tgcagaccat attgtagatg taggaccaaa ggcaggtaag tatggtggag aagttgtgta
     1561 tgaaggcagt tatgaaaatc tacttacaag tggtactttg acagggaatg ctttaagtaa
     1621 atttcttcct attaaagaaa gtgtaagaga acatagtgga tatttagaag ttaaaaattg
     1681 taataaaaac aatttaaaaa atgtatctat taagatacct aaaggtgtat tgactttagt
     1741 tactggagta gcaggagcag gtaaaagtac attgattaag gatgaatttt taaagcaaaa
     1801 tcctagtgct gtacttatag accaaagccc tgtttcagca aattcaaggt cgagtttggc
     1861 aacatatagt ggaattatga acaacataag aagcatattt tcaaaaacaa atggcgtaaa
     1921 tgcttcttta ttcagttcaa attcagaagg agcttgtgat aactgtaagg gttctggaat
     1981 agtagaaatg aatcttgctt ttatggagag tataaagagt acctgcaata tttgtgaagg
     2041 taaaaaattt aaaaaagaag ttttagaata taggtttcaa gggaagaata taattgaagt
     2101 tcttgaaatg tctgttttag aggcaattga gttttttaat ttaaaacaga ttaaaacgaa
     2161 attacaatgt attgaaaaaa tgggtattgg ataccttact ttaggacaaa ctcttgatac
     2221 tttgtctgga ggagagtgtc aaagattaaa gcttgctagt gaattaaata aggaaagttc
     2281 agtgtatata ttagatgaac caacaacagg tcttcatatg gctgatattg agaattttat
     2341 aagtataatt gaagagattg tagataatgg aaatacggtc ataataattg aacacaatat
     2401 agatgtaatt aaaagagctg attggattat tgagctggga cctgaaggag gaacaaaagg
     2461 cgggagagta atatttgaag gtattccaaa acaattatgt aacaataaaa tatcattaac
     2521 agcaaaatat atagtataat aaataaaata taataaaatt ataatgatat tttaataaat
     2581 ataatgggta tataaaatga taattaaact taacagtaat aaataatgtg agaaatattc
     2641 aaatttaatt aaataaagtg tcagaaattt gtttaaattt tagtatttta acggttctga
     2701 ccttttttct tatttttatg gagaatatcg aggatggagt ctctaatagt tacttttttg
     2761 tgtctacttg caagtttgat taaaaaatgg ctatacacac ttgtgacttc agtcatggga
     2821 ggttcagatt ataatcttaa tatataattt gtgaaataat ttgatgacga tttgctgatg
     2881 tataaacaat aaagattaac tacattaaaa tttaaatctt gttaagtaaa acttataaaa
     2941 ggagagagat ggatgataaa tgatattcta accttattat ctgaaagaag tgattttttt
     3001 ataaagctat ccatagaaca tttgatgatt tctattattt ctataataat tgcgattgtt
     3061 ttgggtggta taattggtat tattgtaagt gaatttcata attcttctaa acctatattg
     3121 ggaactatca attttctata caccattcca tctatttcta tgctaggatt tctgatacca
     3181 ttttccggaa ttggaaatac tactgctgtt attgcactta ccatttatgc attattgcca
     3241 atggttagaa atacatatac tggaattact aatgtggata aaaatattct tgaagcagct
     3301 aggggaatgg gaagtacaga ttttcagata ttgtataaaa taaaacttcc gttagcccta
     3361 cctgttatta tgtctggtat tagaaatatg gtgattatga caattgcatt agctggtatc
     3421 gcttcattta taggtgctgg aggcctaggt gtagccatat atagaggaat cacgacaaat
     3481 aacacaataa tgacaattac tggaagtata cttattgctc tattagcttt aatatttgat
     3541 tttattttag ggctggtaga aaaatttttt caacgaaaaa gaaaaaagaa aaataggaaa
     3601 aaagtttttg ccattctagg aagccttatt cttatagtaa ttatctttac gtcttattta
     3661 tttaatcctt caaagactca aactattcat attgctacta aacctatgac tgaacaatat
     3721 attcttggtg agatgttgga tattttaatt gaaaaaaaca caggtctaaa cgtggaaatt
     3781 acacaaggtg taggaggggg aacatctaat atccagccag ctatggagaa tggagaattt
     3841 gacatttatc cagagtatac aggtacaggc tggaatatgg tacttaagaa aaatgaatta
     3901 tatactgaaa gtatgtttag taaactacag tcggaataca aaactaaact taaaatgcaa
     3961 tggactggta tgtatggttt ttctaatagc tatgcattag cagttagaaa agaagttgca
     4021 gataaataca atttaaaaaa tataacggac ttaaaagatg tatcttctaa attgacattt
     4081 ggagctgaat atgactttta tgagagagag gatggatata ctccattgtg taatacatat
     4141 ggactgaact ttaaagatac agttgattta gatattggat taaaatacca agctatcaat
     4201 gaaggtaaga ttgacgtaat gcctatattt acaactgatg gtcagtatag tgtttcagat
     4261 gttgtagttc taaaagatga taaaaatttc tacccatctt atcaatgtgg aaatgttgtt
     4321 cgtttggaag tattagagga aaatccagaa ctatcaaagg tatttaagat ggttggagat
     4381 attttgactg ataaagaaat ggctaaaatg aattatgacg tagaaaacaa caatttagaa
     4441 cctagagaag ttgctcgtga gtttctgaat aagaaaggtc ttttataaat tggaggaaat
     4501 ataatgacac ctattataca atttaaaaat attaaaaaac aatataatga taaaactata
     4561 atagataatc ttaatttaga tatagaaaaa ggagagtttt tgactgtaat tggttcgtct
     4621 ggttctggta agacaacact cttaaaaatg attaatggtc tgattttgcc agatggagga
     4681 aatattttga taaaccaaac agatatcaaa aacgaggact taattaaatt aagaagaaga
     4741 ataggatatt gtgtacaggg aagtgttctt tttcctcata tgactgttga agaaaatatt
     4801 tcatatgtac caaatctcct aagtaaaaag aataaattag aagtaaaaag tgctgtaaac
     4861 aagtggatgg aaatagtggg attgccaaat gatatgaaag tgcgttaccc atcagaattg
     4921 tcaggtggtc agcaacaacg tgtagggatt gcaagagctt tagcttcttc tccagaaatt
     4981 ttattaatgg atgaaccttt tggtgcagtt gatgagatta cacgaaagca attacaaaaa
     5041 gagataaaag aaattcataa aaaaacagga gttacgatta tctttattac gcatgatatt
     5101 tatgaagctt tgattttggg aacaaagact ttggtattaa atcatggagt tgttcaacag
     5161 tatgatactc ctgagaatat tttaaatact cctgctaacc aatttgtaga ccagttattg
     5221 aatataagaa aatctataat agatgatgat aatttaaaag aataaattta aatttaaaat
     5281 caaattgttg tgataaaaac tattgattta gagctaactt caagttgtat acttgaagtg
     5341 gaaaagttag attaaggtat agaagggcat ttagcagttg gctgtagacg aggtaactgc
     5401 tgaaggtgct ttcaataaat ggctagaacc tgtatctgtt gaagattata ataaattgaa
     5461 ataagaagct caatatacag agtagatagg cacttcaaat agaagtgtct tttgtgtgcc
     5521 ataaaataaa tcaaaatatt agcaaagaat gtgaaaaaca aaataaaata atatgctatc
     5581 tttaaaatag gagggatgct atatggataa caatttgatt accattgaaa aaactttaga
     5641 ttatatagaa aaacatcttt tagatgagat gttagattta aatgtaattt ctaaagagat
     5701 aggctattca aaataccatt tacatagaat gtttacatca gttgtcggat ttagtgtaca
     5761 taattatata caaagacgta gacttactga agcagcgaga atgctaattt ttacagaaaa
     5821 accaattatt gagattgctt taatctcggg gtatgataca caacgctctt tttcaagagg
     5881 gtttaaaaaa atgtttaaac gtagtcctaa tttttttaga agacatcaag attttttacc
     5941 actacaatta aaatttgata ttcataatag aaaaaaatta cgtggagata tggttttaga
     6001 tgttaaaaaa gttaattgtg cagatataaa tattgttggc tattctggta aagtaagaaa
     6061 aggatttcat attattggaa aatgctggag ggatttgcat aaaaataaag agatgataga
     6121 aaataaggta gatttaggtt ttttaattgg agttagtgat tatagcaatt ttaaagagat
     6181 tgagaatact cctacattta catacattgc aggtgctcaa gtagctactt ttggcaaatt
     6241 acctaatgga atggaaaaat tcgtattacc gaaaagtaat tatattgttt ttacttttat
     6301 tgggaaaaat gaggatagta tgcaaaatgt tgtagaatat atctatcaag aatggttccc
     6361 taattcaagt tgccagttta atgaaaataa tttatatgac tttgtaaaat atggagaaaa
     6421 aactgatgag aatggtttga gtgaaattca gttttgggtt ccaatcattt aaaagaaggt
     6481 attatgtata aagtagatgg atttatatag gcaaagtttt acagcagtat gtatttataa
     6541 ataatttata gtttgtagga aagaaataat tttactataa gattatgata aaatacacaa
     6601 tactatgaca aaatagtgtt aaataataga tttaattttg cataggaaaa gttctataat
     6661 ggtgtgtatt cataaataat ttataatctg taggaaagaa atagttttac tataagatta
     6721 tgataaaata cacaatacta tgataaaata gtactaaata atagatttaa ttttgcaaag
     6781 gataaggtgt tttaataaaa atgaatgtag aaaacgaatt acttaaaaac ctagatagat
     6841 tgcatacaac agagttaggc gttgtaagaa ttaagaaaaa tctttctttg gagacaaatg
     6901 atgtagtgaa ctggtgtaaa actaaaatag aatctccaaa tgccattatt aatagaaagg
     6961 gaaaaaactg gtatataagt gtatatgatt gtattataac agtaaacgct cacagttata
     7021 caattattac agctcataaa gaaaagaaat gaattgcaaa atacaaaggt caaaagcatt
     7081 ttccactgtt tgtagtacct atgaagaagt atataaaata atattacttt tgaataataa
     7141 tttaaatttt ggaggttgaa aaaatgaatg atactgtaaa agaaaaattg gcagagtcac
     7201 taacttctga tactactgat attttacctt atttgcctta tttattacaa gatttttggg
     7261 aattaggaag ttcaccagaa caaatgttac aattatttag aaagcatata ccttttaatg
     7321 aggatacaaa agttttggat ttagcttgtg gaaaaggtgc tgtatctgtg aagcttgcaa
     7381 aagagttgaa tatacatatc aaaggtattg atttgatacc tgaatttatt aattatgcta
     7441 gaagaaaggc tattgaatat aaggttgatg ctttgtgtga ttttgaaatg aatgatgcta
     7501 atagttctgt aaaaattgag catgattatg attgtgtgat ttttggagct gtaggggata
     7561 ttttaggtaa ttataaagaa actataagta agctattacc tacaataaaa agtaaggggt
     7621 tcattttaat tgatgatgca tatgtagtag ataaatctac aaatgaacag cttaattttg
     7681 agagggtgta tcccacttac aatgagtggt gtatattgtt caaagaatta ggtctgaagc
     7741 taattgaatg tataccatgt ttagaacttg aaaatgatgt tgaagaggaa atgaattgga
     7801 ttataactcg tgcaaatgag ctaattaaaa aatatccttc tcaaaaagat atgtttgaac
     7861 aatatgttat tagccaaaaa aatgaatata ctgatttaca gcaggacctt gttggagcaa
     7921 cttggatttt acaaaaagaa taagataaat attagactag ataatcaatg ttatagtgaa
     7981 ttatcccatg tctagtgaat ataaataaca taaaaaaccg tcaagatgaa tagtttattc
     8041 gtgcttgacg gtttttagta tttgttgaag taaaatttta tttctttagt atatcacttt
     8101 tagtcaatcc atttgattta gcttgagtat tccattgatg attccccata agtttatata
     8161 ggaatctagg gaagttaggt gaaataaaaa tagtactttt gttggaatca tctatatgta
     8221 tattatcact aagtatattt tttgaaagtt cttttaatgc aattcctaaa tcttttttag
     8281 gtcctttacc caaaggtacg gattctagtg aaccaaacat ctccccagct ccaattccaa
     8341 taccttgaac ccatgtaaag ttgatttttt tacaccaatg ctctatcatt tgaattgcca
     8401 aatgattatg ttcaccttca ttgaatccac agttaactat tgcatacact ggaataggtt
     8461 ttttattttg tgtttttaaa aatttttcac atttctctaa aaaatgaagt aaatgagatg
     8521 gaataccatc tacataaaga ggagatgcga ttattaatct atcacaatta agtatggcat
     8581 taagagcgtc aggatactta gatgtgctat ctactttaaa tgtaaaaact ttattatcat
     8641 tttgaataag ttgttttaat tcagataaaa aatactgtga attattttta tttacttttg
     8701 gactaccatt tatcattact atattcatat ggattcacct cttatatctt gtatattttc
     8761 atagaaacaa acagagtgtt cttttatatt gaaatttata gcatttccat ttactaattt
     8821 cgtagcagtt tctttttcag cttctgtaat acaatctcca tagaatatag cttttaaatt
     8881 tatctgttta tcatatctgc ttttatgacg catcatatta tcttttatta caaagtatgg
     8941 aagaatataa ttaatgcttt tatcaagtac gtttttgaca aaagggctaa accctccgta
     9001 acagcatctg cttattataa taattccatc agagtcattt ataagtttac ccatgttgct
     9061 gtaataatct tttacaacac attcagaagg agttttaatc cagcaaccaa aacaaccaat
     9121 acatttgtgg atttcttttg tgtttggaag tatttttata ttgtcatcaa gtttgggtaa
     9181 taacttttct aattctttgt cagttaaatc atgtattaaa agcctcataa agtccatctc
     9241 cttttattat gtatgtttac ttctaaaaat tatatcttat agatttttca gtaaatcttt
     9301 acaccaatta ataacaaaat tataatagtc aactccataa atcattgttg catacatata
     9361 tgtatattgg tcaacgcaat gattttcttc tgcatcattt ttttgtttac ttaatttatt
     9421 tagaagtaat tctacttcag ttattaacat ttttaagttt gtttcaatta tttctttggg
     9481 tagatacttg taaaagaata tattaacaag gtggtcttgc tttgttttag aaaaatttat
     9541 aggtttcttt aaccattcaa gaaatacttc tttaccagta ttagttatgg aatataattt
     9601 ttttaatttg ctaccatcta taacttcgtg atagtgtata tatccttttg tttctaatct
     9661 tttaagtgca ggatagatac ttccaaagct ggcgtcaaaa aagtacgagg tactttctga
     9721 cattatttgt tttaagtcgt atccacttaa ttctttttcc attaaaaatc ctaaaataat
     9781 gtattctaac atattacctc ctggtatcaa ttagatatat atcgattcga tatatttatt
     9841 attacagata ataaatctta cgtcaagatg ctttttaaga taagctaaaa agaattagga
     9901 gttattgata aatatgcaaa aaaattaata taaaaggtaa ttaaatatca atgaagattt
     9961 aaaaatagtt aaatcaaagt aagagtctaa tatataaaat ttatagtaac tttatggttt
    10021 aaatataaac ttgcgtgcta ttgacaaagt ataaaattag cagtaaaata attgcatgag
    10081 caaatagatt attgcgaacg caaaaaaata aggacaatag atattaaagg agagaatatg
    10141 agagagctat taaaatgggt ttccattata aatagataca atacaaatta tattgacagg
    10201 aaattggcgg atattggaat caataatagc caatattttt atgtattaca catatgtaat
    10261 aatccaggta ttacacagga cactatattt gagaaaattc ttgtgaatcc tagtaatata
    10321 acaagagctt tggcaaattt agaaaaagaa ggctttataa caagagaacc aggtgtaaag
    10381 gataaaagaa catggcattt atatcctaca gaaaaatctt taggatgtta tgatgaaata
    10441 ctaaaaataa caaatgggta tgttgaggag ttgttgagtt catttgagaa agaagagcaa
    10501 gagttgttta tgtcaatgtt aaagaaagtt gcatttttgg ctattgatat gaatgaagaa
    10561 gcaaaattgg aggcgaagga aaatggaaga aaaatataag gagccattgg aatatgaaaa
    10621 ggtttcaaag ttattaacta agtacgcaat tcctagtatt atagcaatgt tagtaagtgc
    10681 tttatataac attgttgacc aaatatttat tgggcaaggt gttggtgtat tgggtaatgc
    10741 tgctacaaat gtcacttttc cactaacaac aatatgtact gcaattgcat tattgttagg
    10801 aattggagga gcgtctaagt gttcattgga attaggtgct aaaaattcag aaaaagctac
    10861 aaaagcagca ggaaattcta tatgcttgat ggctgttttt ggaataagtt tatttatagt
    10921 agtatcaata tttttaacac cactattaaa attctttggc tctacatcag aaatactacc
    10981 ttatgcacac acatatacaa aaattacatc aattggatta ccatttctta taatgtccac
    11041 tggaatgagt aagttgatac ttgcagatgg gagaccaaaa gcttctatgt tttgtatgtt
    11101 aataggagca gttattaata caattttgaa tccattattc atttttgttt ttaatatggg
    11161 aattgctggg tctgctttag caactgttat tgggcaactt atatcttttg gaataagtgt
    11221 tcattatata aagaattttc aacatattaa attacataag aaaagtttta aattagatag
    11281 tataatttat aaacatatat ttgcgctagg ttcaagctca tgttttaatc aattagcaat
    11341 gactgtaaca cagatagtta tgaataatac attggcatat tatggggcta ggtcagttta
    11401 tggaagtgag attccacttg cttgtgtcgg tatcattatt aaagtaaata tgatttttat
    11461 atctataatt attggtatat cacaaggtat gcaaccaatt atagggtata attatggggc
    11521 tgaaaagtat aatcgtgtaa aagaagcata taagtttgca gtgggaagtg caactattat
    11581 atctgttatt gcattcttat gtttccaatt cttcccaaga caaattacgt ctatatttgg
    11641 taacggaaat gagacttatt ttatatttgc agaacattat tttagaactt tcttgttctt
    11701 aacatttata aatggtgttc aaatagtatc atcaactttc tttacatcaa ttgggaaatc
    11761 agtgttggga ctttttactt ctttgactcg tcaaatttta tttttaattc cattgatagt
    11821 aattttacca atgtttatgg gaattgatgg agtaatgttt gcaggaccaa tagcagatgc
    11881 agcagcggca atggtatgta tatactttgc agttcatgaa atgaaatcat tgactataaa
    11941 gcaaaataat ttgatgagta accataataa gattgcagag gtataagaca atatttaaga
    12001 agtatagaaa attaatttaa aaaatataat aaattaattt aggaagtgta gaaaattaat
    12061 ctaaagaagt ataataaact aatttaggaa gtatagaaaa ttaatctaaa gaagtgtaat
    12121 aaactaattt aggaagtata gaaaattaat ttaaagaagt ataataaact aatttaagaa
    12181 atatagaaaa ttaatttaaa gaaatacaat acattaattt ttaagtaaca ttaaattaat
    12241 gtaagtaaaa taatgttcat atgaatattt aaaggtgcat tttctgaagc taagtatatg
    12301 ttgtagagtg ttatgtctct ctatcctgtg aagtttaata gctcaagaaa atgcactatt
    12361 tatgatgatt attttgtttt atttccaatt ttggaaataa ctattctaat taaatctaaa
    12421 attataccat aaataagaaa agaaagtata gatgctatgt accaataaat cgaaattgac
    12481 cctgtaattg ttctacatgg gatttgcata cctgaatcca taaaaacaaa aaaactatca
    12541 actctggaaa taatagacaa aataggattt aaacctatta gaacaatact ttttgagtct
    12601 tgaccaattt gatgcatata aatctcaaat aatgcaatta aaaaaaacca agatgtaaaa
    12661 gatttaataa tttttttcat caaaatacct cacataatat aaattctgtt tgcactattt
    12721 gcttactaca actatttatc tccattcagt cacatcactt aattgtaatc taggtcttct
    12781 tgatggttct tcatcaggat aacctaaaga tattgctgcc actaattgac tatctgtatt
    12841 taaccattca cataattcac gatatgcaaa gtatacatca caaatccaaa gactacctag
    12901 acctaattcc acagctgtaa gcgacatatt ctgaattgca gctccaattg attgcatatt
    12961 tgccatttca tagaatcgtt cttcagggga tagcttttct aaaggtgatt tacctaatat
    13021 atttaaaata aaaattgtta ctggagcttg tttcatgatt tcaactgtgt aatttgcacc
    13081 agcgatatgt tgacgacttc ctggaagaag cccattatca ttaatttcat tttgaatacc
    13141 tttgctcatg gctttaagca tagattcttt ttccttttca gtgatgacaa caaaacgcca
    13201 tggttgcctg tttttagatg aaggtgcatt tattccagct tcaattattt tctctatagt
    13261 ttcatgtgaa atgctttgat ttttatattt tctgatactt cgtctttttg atatagaatc
    13321 acttatcatt ttaagtaacc tccgtttatt ttttggaaaa atctatttaa tttttatttt
    13381 ctattttaat tattttagca ttaatcagaa aaaaagtcat tgattttagt atacaattat
    13441 taatgattat attaagaatt gtatcaaaaa gtatgtgatt atactaaggg attatattaa
    13501 acattatatt taaaattgta tgaaaggt
//
LOCUS       DAYAOX010000104        13442 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.104, whole genome shotgun sequence.
ACCESSION   DAYAOX010000104 DAYAOX010000000
VERSION     DAYAOX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.104"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            201..1277
                     /locus_tag="ACGI57_002258"
     CDS             201..1277
                     /locus_tag="ACGI57_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFG9717334.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1380..2222)
                     /locus_tag="ACGI57_002259"
     CDS             complement(1380..2222)
                     /locus_tag="ACGI57_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFG9717335.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2406..2978
                     /locus_tag="ACGI57_002260"
     CDS             2406..2978
                     /locus_tag="ACGI57_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9717336.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3141..3413)
                     /locus_tag="ACGI57_002261"
     CDS             complement(3141..3413)
                     /locus_tag="ACGI57_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717337.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3540..4412)
                     /locus_tag="ACGI57_002262"
     CDS             complement(3540..4412)
                     /locus_tag="ACGI57_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9717338.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4708..7815)
                     /gene="ileS"
                     /locus_tag="ACGI57_002263"
     CDS             complement(4708..7815)
                     /gene="ileS"
                     /locus_tag="ACGI57_002263"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFG9717339.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7908..8140)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8315..8842)
                     /locus_tag="ACGI57_002264"
     CDS             complement(8315..8842)
                     /locus_tag="ACGI57_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFG9717340.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8877..9662)
                     /locus_tag="ACGI57_002265"
     CDS             complement(8877..9662)
                     /locus_tag="ACGI57_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HFG9717341.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9672..9929)
                     /locus_tag="ACGI57_002266"
     CDS             complement(9672..9929)
                     /locus_tag="ACGI57_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFG9717342.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9948..10406)
                     /locus_tag="ACGI57_002267"
     CDS             complement(9948..10406)
                     /locus_tag="ACGI57_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFG9717343.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10446..11150)
                     /locus_tag="ACGI57_002268"
     CDS             complement(10446..11150)
                     /locus_tag="ACGI57_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFG9717344.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11160..11795)
                     /locus_tag="ACGI57_002269"
     CDS             complement(11160..11795)
                     /locus_tag="ACGI57_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFG9717345.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12176..13216
                     /locus_tag="ACGI57_002270"
     CDS             12176..13216
                     /locus_tag="ACGI57_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFG9717346.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT         4291 a         2264 c         1585 g         5302 t
ORIGIN      
        1 agacttaatt tattactttt atagaatttt cacaataaaa agtcatttta cttccgtatt
       61 taatgcaaaa gtagaaatta aacttaatat aaaaaatata taacaaattt tttttatatt
      121 tctagaagaa aagtatactc ttttttgttt aataatgcta aaattgattt gtattgaatt
      181 ttgattagga ggaaattatt atgaaagctc aaagattaaa tgctgtactt gaacaaatga
      241 aaaaagatga tatttctcaa atgttagtaa gtgaccctac ttctatattt tatcttacag
      301 gagtactaat ccatccaggc gaaagactat tagcattata cttaaattta aatggcaata
      361 ataaattatt tataaatgaa ttattcccag tttcagaaga tttaggtgtt gaaatggttt
      421 ggtttaatga tactcaaaat ccagtagaaa taataactga acatatagac aaaaatgcta
      481 ctatgggtgt tgataaaaac tggccagcta gattcttatt aaacttaata gaattaggtg
      541 gaggaagtaa gtttgtaaat tcatcttata taatagatac tttaagaatg tgtaaagatg
      601 aagaagaaaa agagttaatg agaatagctt caaaattaaa tgacaaggct atggaacaac
      661 ttaaagctac tgtttctggt gaacttacag aaaaacaact tgttggtaaa ttatctaaaa
      721 tatatgaaga cctaggtact gatggatttt catttgaccc tattataggt tttggaccta
      781 atggagctaa tccacatggt gaacctggaa atgcacttgt gaaaccagga gatgcaataa
      841 tcttagatat aggttgtata aaagataact attgtgctga tatgacaaga actgtattct
      901 ataaagaaat acctgaaaaa ggtagagaaa tatttgaaat agttcttgaa gctaataaga
      961 gagctgaagc tattgtaaaa ccaggtgtta gattctgtga tatagatgct gctgctcgtg
     1021 actacataac tgaaaaggga tatggacagt acttcactca tagaactggt cactctatag
     1081 gacttgaagt tcatgataaa ggagatgtat cttctataaa tacagatact gtacaaccag
     1141 gaatgatatt ctctatagag ccaggtatat atttaccagg tgagttcgga gttagaatag
     1201 aagatttagt acttgttact gaagatggtt gtgagatatt aaacaaacat gacaaagaaa
     1261 tatgtgtagt tggttaattc taaaatcaat ttgataaaat taaaaagcca tataaaaatg
     1321 ttttaaacat ttttatatgg ctttaatttc tcaatctatg atttatttca atccattatt
     1381 tatattgcag ttattaagat tgcacccttt gaagtatatg tccccatcac tgaaccaatt
     1441 tcacagatta caacatcttt gtattttaca tccttttcca taacttccct taccttttga
     1501 gccttttctg ggcaattagc atgtcctata aataaaacct tttcttcttt atctttaatt
     1561 tttgactcta ctaaagtaat cagctttttc aaactgtttc cttcacctct tgccttatct
     1621 ataggagtta caacgccatc tgcaacttgt attatcgcct taaaatttaa ggtatttata
     1681 atttttcctg ctattggatt tattctacca cccttaatag cattttctaa agtttcaagt
     1741 gttccaaaaa atggaggatt atctttatat ttatttatta ttgaaacaat ctcttcaatt
     1801 tcttttcctt cttttattaa tattgctgct tttaacacta attgtcctat tccaattgac
     1861 ccactaagtg aatctataac ctcaactttc ttgttatttc cttccttttc aaacatatct
     1921 tttgctaaaa gtgcactatt ataagtccca gataatcctg aacttatatt aatcactaat
     1981 acactttctt cctgacaatg gtatgcttct aaaaatttat caggagaagg acatgatgtt
     2041 tttggtagta ctttactctc actcattttt ctatagaaag tttcattatc tatttccttt
     2101 ccagatatat aactttcttc tccaaaagat acatttaaac caacaacttc tatgttgtat
     2161 ttttctatta tatcatcagg taagtcacat gcactgtcca ctatcaattt tattttatcc
     2221 atattaacct cactataaga tattatttta tttattcagt cggtcgtacg accgatttaa
     2281 ttattatttt atatattgaa tgaaaaaaag tcaatagata tagattctcg ctttattgac
     2341 aataaatatc cataagctta taatgttatt aatttgttat aaatatacat ggaaaggtag
     2401 gttttatgaa aacaaataat atgaaacagg ctattttatc atctgcaacc aatttaatag
     2461 ccaaaaatgg agttcaaaat actagccttg ctgatattgc aaaagatgta aatataagta
     2521 aaggtacttt gtattatcac tacgcatcta aagatgatat tatatatgat attgcggata
     2581 ctcatttaga agttataact agtgctatac taaactgtgt aaaaaatgtc aaatcaaaaa
     2641 attctcaaat agaaatggtt aacttaatct tagagaaaat atctactata gaaagtcgtg
     2701 gtagggtaca tatgtatctt atttgtgagg caattacttc aaataatgat ttaaaggaaa
     2761 gaataagatt aaaatatatt gaatggagaa ctactcttaa atctgaaatt gttgaatcac
     2821 tagaaaaata taatagtagc gaaaatgaac aggatgctga atcattttct tttttactca
     2881 tgtcaatagt tgatggttta gttgtccaaa gtttattaaa aacagaaaaa ataccttatg
     2941 aaaatatagc ttctttttta gttaatcatt ggatttaaac ttaatactta gataattaat
     3001 aaaagtgaac tatttaagtt atctacttta acagttcact ttcttattta ttctcaataa
     3061 tttttactta aaacattcaa tttttattta aaccttcatg gaatcacaaa ataaaaatct
     3121 cttataaatt taaaatgttt ttatttatgt tcatgtgtat gcccatctga atgttcatga
     3181 ccatgtgtat gtgtatgctc atggctatgc tcatgagtac ctgaaaaacc atcatcatga
     3241 ttatggtcgt gacctacacc atttacatgc tcatgttcat gcccatgact gtgagaatgt
     3301 tgatgtgaat gttcatggtt atctcctcca cagcatcctt ctcctccagt cccacaacac
     3361 ccacttgtat gtggattatg atgatttctt actacaaatc tctctcttaa cataattctt
     3421 atacctccta gtatattatt gagtacttca tgtaccccat taactacagt atacatctaa
     3481 ctaggtataa tctcaataca tatggcattt tttaacatat ataattaaca tatataatct
     3541 taaagaaatt tatccttaaa acctccacct tttacttctt caacgttatt gacagaaata
     3601 aaagcttcac tatccatatc ataaattaaa tctttaaatt tacctatttc atttgcagat
     3661 attatgcaat aaatcatctt tttcttattt tgtgtatatg caccttctgc ctccaaaaaa
     3721 gtgactcctc tacccatttt attcattatc actttggata tttcatcata tttatttgat
     3781 attaaaagta tagatttttt tctatcaaag caatcttttc ctatatccat agtaaaagaa
     3841 attatataca ttgtaattaa agtgtacatc accaatttag ctccaaataa aaaagctcct
     3901 gcacatacta caaaaaaatt aataaataaa aatgtatttt tcataggtat atcatacttt
     3961 cttttcatta tggctgctat tatatcaagt cctccagcag tagctcttgc tttaaaagtt
     4021 aaccccatac ctataccagt taatgcacca ccataaatac actgtagcat agtatcatct
     4081 attgggaaat atttagcaat atcttgtgtt agccccagtg ctagagaaaa tagtaacata
     4141 ttgacaaaac ttattagcaa aaatttatta tcaaaatact ttctagctat aataaatatt
     4201 ggtatgttca tcaagaaact tgatattccc tgattaatac caaataattt gtataatata
     4261 acacaaattc cagctactcc tccacttaat aacttggctg gtgctagata taaatttatt
     4321 cctaaggttg atagaatcat acccaatata ataagacaat attgtaaaaa tatattatta
     4381 tcaatactta gctttttact ttcaccatac atcaaaacgc acccctttgc ataataaata
     4441 aaccaccctg cataacacac gataaattta caaataaatt tatcagaagt agtttcctaa
     4501 aaatattatt catcctcact taaacgttta ttcattaata ttctatcaca tgacataaat
     4561 aatttatatg atttatatag atttatttat tgtaaatttt ggaatattaa aacattttat
     4621 tgaatagata attatattac ataaaaaatc cctatagaaa tctataaact aatttctata
     4681 gggattttgg ttaactaact attattttta tttttttata acttttatac ctgtcatatg
     4741 gtcatttaag ttttgttcct ctaaagattt atcatctact ctgtttattt ctaaagctaa
     4801 agtttcagac ttgatatatt cattaaaatg gtcaacagcc ttagcaattt catcatcacc
     4861 acagtattct attactatat tatctaatac ttcaaagtta ttagattttc ttaattgctg
     4921 tactttagat ataaattctc tagcataacc ttcattaatt aactcttcag ttagcttagt
     4981 atctagtata acaaaaagat tgttttcaac agatacatta aatccttcct tagcagttat
     5041 acctattaat actaaatcct tattaaattc aaaagcttca ccatctaaat caactgttaa
     5101 agtctcacct ttttcaagtt tattagcagt ttcatgagca tttaatttac ttaatgctcc
     5161 aacgaataaa ttcatcttag aacctaataa tggccctgct tccttaaagt ttggttttaa
     5221 actaaagttc atatactcat ctaaatcctt agcaaatatt acctctttaa cattaagctc
     5281 ttcttttatt aaatcaacta catcacttat tgtatcttca aacttaccat ctactaacac
     5341 tttttgtaat ggttgacgaa cttttattct ttctacttct cttgaagctc tacctaaagt
     5401 aactaagttt ttagctaaat ccattttttc ttctaattta gtatctacta actcttcatt
     5461 acatttagga tattcagcta aatgaacaga tacttctcct gttaagtttc tatacatttc
     5521 ttctgacata tatggtgcaa atggagctat agctctacaa agaccatgta acaattcata
     5581 agttgtattg tacactgctt ttttatcttc tgttaattca gttgcccaga atcttcttct
     5641 tgaacgtctt atataccagt tagataaatc atcatttata aaatcttgta tcattcttaa
     5701 agttttattt aactcaaata tagctaaatt ttcttctaca tctttcatta aattattgaa
     5761 cttagataat atccatctat caagttctgg tctatcttta tattctacaa aaaattcagt
     5821 tggatttata tcatctgtgt ttgcatataa agtaaagaag ttatatacat tcttcatagt
     5881 acctataaat ttactttgga tttcttttaa tccatccata tcaaatttag ttggagtcca
     5941 tggtggagaa acatatagta aataccatct taatgcatct gctccatatt ggtcaaataa
     6001 ttccattgga tttactgtat ttcctcttga tttactcatt ttcttaccat ctttatcaag
     6061 tacaagatct ggaactaaaa cactcttata tggtgctttt cctaatacaa atgttgatac
     6121 tgcaagtaat gagtagaacc aacctctagt ctggtctata ccttctgata tatagtcagc
     6181 tgggaacaat tggtcaaagt tttccttatt ttcaaatgga tagtggtgtt gagcaaatgg
     6241 catagcccca ctatcaaacc aacagtctat aacctctgtt actcttgtca ttggttttcc
     6301 acatttttca cattttaagt gtatatcatc tacatatggt ctatgaagtt caactgtttc
     6361 tggatttaca tcttctatag ctttttcagc taattcagct cttgaaccaa ctgaatctgt
     6421 atgaccacat tcacatctcc atataggaag tggagttccc caatatctag ttcttgatat
     6481 tgcccagtca tttagatttt ctaaccagtt tccaaatctc ttctctccta caaaacttgg
     6541 gaaccatttt acaccattat tattttctat taatttatct ttaagtttag tcatctctat
     6601 ataccagctt ggttttgcat agtatactaa tggagtttga catctccagc agtgtggata
     6661 attatgaact actttttctt tgctatataa tttattttca gaagctagcc attttattat
     6721 ttcaacatct agcccatctt ccattacaaa tctaccttcc caaggagtag atgtaaattt
     6781 accagtttca tctactggtt gaataactgg taaatcatat cttttacctg tgttgtagtc
     6841 atcttcacca aatgctggtg ctgtatgaac tataccagtt ccatcttcag tagttacata
     6901 atctcctact gtaataaaaa atgctttttt gtcaacttct acaaaaggca ttaattgttc
     6961 atattctaaa ccttctaggt ctttaccttt catttcttct aaaacttcat aatcttcacc
     7021 tagtacttta tcagctaaag gtttagatac ataatatacc tcatcacctt ttttaacttt
     7081 taaataatct acatcagcat ttactgttaa tgaaacgttt gatggtaaag tccatggagt
     7141 tgttgtccat gctaagaagt attcatcagc atcttttttc ttaaatttag ctataactgt
     7201 attagttttt acttctttat atccttgtgc aacctcatga ctagcaagac cagttccaca
     7261 tcttgggcaa taaggaagta ttttatgtcc ttcatatata tatccttctt tgttgaattt
     7321 gtttagtatc caccatacag actctatata gttattatct aaagttatat atggattatc
     7381 taaatcaact tcataagcca ttctctcagt cattattctc cattgttttt caaatgagaa
     7441 aactgactct ctacattttt ggttaaattc agctattcca tattcttcta tttgttgctt
     7501 gctagtaaga cctaactctt tctctacttg tatttcaact ggtaatccat gtgtatccca
     7561 accagctttt ctctttactt gataaccaga cattgtttta tatctacaaa ctgaatcttt
     7621 aagcgttcta gataaaacat ggtgtactcc aggatttcca ttagctgttg gaggtccttc
     7681 atagaatacg aaacttgggt catctttccc tttttctaaa gttttttcaa gtatatttat
     7741 gtctttccaa taatcaaaaa cttgtgcttc tgcttgtttt acagaactgt ctaccaatgg
     7801 cttaaatttc gccatgtaaa tcaccctttc ctactaaata ttataattcc aaaatttttt
     7861 attctttcac ataaatttaa atactaaaaa actcgccccc tttaaaccaa agggacgagt
     7921 tataaatcgc gttaccaccc taattctata tgtaaactaa atactagcta catataacac
     7981 tctaatagta taacggtact aaccggcaca gcttagtaga atttaatcgt tcagcctgca
     8041 gctcaagagt gatttttatc tttataaatt cattggttct cagcataccc aattctctgt
     8101 aaaattatct aaagataaat gtctctatca acgcttttaa aaatttatct gtgtttatat
     8161 gaaattctat aacaaataat ttgaatagtc aatatatttt tgaaaaatat aataatttct
     8221 atttttctta catttttatc ctatttttaa tctttgttat agatttctca aactcacaca
     8281 attattctac ataaaaaata aacaaattac aagcttattc taaagttgta gcagcttcat
     8341 cattactata attatacact gttgttcctt caaatgcatt tacacatttt tcatctacat
     8401 cataaaaaat ctcatcaaca cttcttattt catcttctaa cagtgattta aatttatttc
     8461 taaatatctt aaattctttg actaaagcat catactcttt tcttatctct ataacacggt
     8521 tattagcttg ttctataatt tgtctagctt ttaaatcagc ttcttcaaca attatcttag
     8581 cctttttatt agccgcgcta caagtgtctt ctgctgcact ttgagcagtt attaaagtag
     8641 cttttagtgt ttcttcaata ttttcatact tgtttacttg gtcttgatat aaccctaatt
     8701 tttctttaag agctgtattt tccctacata gagattcata atcttccttg atgatatcta
     8761 aaaattcatc aacctcttca tctctatacc ctcttaaacc ttttttaaac tctttatttt
     8821 ctatctcaat tggagttagc attacatttc ctcccagcta taatttaaat tagtccttat
     8881 acaattaatc ttgcttgaac ttttattttt ccttttttag taatatctcc tatttgtgtt
     8941 accttagctc ttcctttgcc tctaatagaa atcaaatcat cgttttttac tattttagaa
     9001 gttgaagtta tcttttcata attgacatgg actttttctc catttatgta ttttgcacta
     9061 tcttgtctag atatattgta aataccactt atcacacaat ctaacctatc tgaagatact
     9121 gtaaatgata cttctttata ttttgaagaa ctattaacaa tattttctct ggaaatttcc
     9181 tttacaatta cattatttct tgaaaccttt tctaagttta caattataaa atcgcaaata
     9241 tcactactta caactacttg acaaaagcta tcatgtataa ttatatctcc aattttttct
     9301 ctttttatac ctagacccag tattgaccct aagtaatctt tatgagatat atttttaaat
     9361 ttgaaattcc cttctatttg taaaaatcta agagtatctt ctatatcttc atattccatg
     9421 taaaatggat aaataaatac agtgcttctt tcagcttgtt cataacctcc atccacactg
     9481 tattttattt catttgtaga atttaatatt gctacagcat ttttcacttc atatggatta
     9541 agaaactccg tagacttaac atcatagttt tttatacaac tattagcctt gtctatcact
     9601 ttaaacattt tatttttcaa gtctatatcc tttatgtgat ttgttagttt taatttatcc
     9661 atcctatcac cttagaatat aaccaatact aattgtctta ggaacatcaa taatactatt
     9721 gcaattaaag gagaaaaatc tataggtcca tttgtatatt tatacattat agaccttatt
     9781 ggcccttcta ttggctctgt aagattttcc aaaatatcaa aaattttact ttgtcttcca
     9841 tttggaaacc aagtcatcaa gctttttata actatcatcc atgctattat atctaaaaga
     9901 taataaagtg caattcttat tgttcccata tattaaccgc ctttttacta tttattttgc
     9961 caagggaaga atgctttact ttctaattct tttttcatat ttgcatctat atccacatta
    10021 ttaggagcta gtataaatat tgatttagaa accttttgta tacttccatc taagacataa
    10081 actgccccat tcataaagtt aaatatagat ttacgaactt cagaattagt caatccttcc
    10141 aaattcacta taactgccct tctttgtttt agatggtctg ctatagttgc agcttcatca
    10201 taaaccttag gttcaactat tacaactttc atttggctag ttgtatgtaa atttacaatt
    10261 ttatttgctt ttgcagtact aaagccactg ttcatttctt cttcttgaac tatatcgtcc
    10321 atgccacttt catattcatc ttcaatgtaa tcttcttctt catcaactat ccaattctta
    10381 aatttagata ttattccatt tgacattatt aacttcctcc ttaaaattta ttcaaatttg
    10441 agtaattatt ttttattgta gtttctttct ccaaagattg atgttccaac tctaacaatg
    10501 gtagcccctt cctctatagc aatttcataa tcgtgactca ttcccattga caaaacatcc
    10561 atccctacat tagggatgtt tagttctttt atttgttttg ataaatcttt aagaccttta
    10621 aatacacctc ttatctcatc ttcatcttct ataaaaggtg ccattgtcat tagacccttt
    10681 atttttatat ttttatattt ttctgacact tctttgacaa agtctacaac ttcttctcta
    10741 tcaagtccat gcttagactc ttctttagat atattcactt gtactaagca atctatttct
    10801 ctgtttattt gttctgcacg tttttgaatt tcttcgcata atgcaattct atctaaagag
    10861 tgtatcatac aaactttatc gattatgtac ttaactttat tggtttgcaa agttcctatc
    10921 aggtgccagt ttaatttatc acctataata tcatactttc tagctaattc ttggggttta
    10981 ttttcaccaa cagaagtgat tccttccctc attgcctcat taactaagtc tacatcaaca
    11041 gtctttgtga ctgccaaaag ggtaatctct ttgctgtctc tatctacttt ttttgcagct
    11101 ttatcaattt tgtttcttat ctcagacaaa ttatctttaa tagaacccat tattatcacc
    11161 tactttatcc ttatatttgt gtttatactg ttaggtctta aaattatttt gtcatataga
    11221 ttaacacttg taactccatt tatgtcattt ttataataat ccacaaatac agaagattca
    11281 ttctcataag ttattccttc taatatgaca aattttacac tttgtgattg ttcatcaatt
    11341 acatatacac cacgtttatt gtcttttgtt tttatagaag ttttaggtat ttttaaacat
    11401 tccatctgat tatatattat atcaaattct tcgacacttg tatcctgatt tgcaacaatt
    11461 tggttactta tttttaattt taccattatt ttattattat tttcatatat tttgtaaact
    11521 tttgcatcta gtttttgctc atgatattca attattacat tttgattttt ttcaaagtta
    11581 ttagaatctt ctttactgat gtatgtagat agataattac tttggtcatt ggcatctttt
    11641 tctatagttc cttttacatg aatctttgca tcaacttttt cattttttaa caccaacgta
    11701 tctacatttt tgctaattat cattactaat atattaaata ctaaatatat aagaatacca
    11761 agtatcacta atctggtata ttttaccttt ttcaaagact ctccccctat aaaattactt
    11821 tcattataag tacttcttct aaaaaatgtt tattcctttt ttataagata atattttacg
    11881 ttctaataat tattttttcc atattaaatt tattttttat caatcttcct atacaagact
    11941 gcattattta tatataattg tattataaag tataagttta atttacattt tatacgttaa
    12001 gcttacttta tatattttta tagttatttt tatttttaga tgcaatataa aaccaattaa
    12061 gatttaaaat aacttttgtt atgttgacat ttatttaggt aatcatttat gaattaggat
    12121 aacgttatca caacaaaagt ttatgtccta aaaaaaatac aaggaggatt ttcttatgtc
    12181 agaaatttta ttaggaacag gtttactttt attggttcta tgttgtttca caatttttag
    12241 ttacaaggcc ccatatggta tgaaggctat gggagctcta gcaaacgctg catgtgctac
    12301 tttcttagta gaggcattca acctttcttt acttggtgat gttttaggta tggaattttt
    12361 tagaagtgtt ggagctgcta atggtagtct tggtggagtt gctgctgcta tacttgtgcc
    12421 tttagcctta ggtgtatctc cagtttatgc agttttaata ggattatctt gtttagattt
    12481 ttctatatta tctggattcg ttgctggata ctttgtttct ttcttaatta agtttataga
    12541 gaaaaaagtg ccacctggag tggatttagt ggttgtaatt cttctatgtg caccactatc
    12601 tcgtcttata gcatcaaatg tagacccatt agttaatagt acactactac aaattgggca
    12661 agttttgata gaagctacca acattagccc tataataatg ggaattatac taggaggtct
    12721 aataacagta gttggaacgt ctccacttag ttcaatggct cttacatcta taattggtct
    12781 tactggtctt cctatggcaa taggagcatt aggtgctttt ggttcagctt ttatgaactc
    12841 aacactattt gcaaaattga aattaggttc taaaaaagat attatatctg ttgctattga
    12901 accattaacc caatcagaca taatttcagc taatccaata cctatatatt ctactaattt
    12961 ctttggagga gccttttctg gtatcattgt agccttgatg ggtcttgtaa acatgacacc
    13021 aggtacagct acacctgtgg ctggttttgc tataatgttt gcttataatc cagttcaaca
    13081 agttcttata acagctggtt tatgtgcttt attcaatgta attgcaggat ttttaggttc
    13141 aaaaatattc agtaccttta aagttcttac tgctggtgaa atacgtggta ctgatgtaga
    13201 agaagaaatt gcttaaaaaa caataagatt tataaattta tatctcaagt agtagagttt
    13261 tatattgtag gcaattttat taattgcaaa cgttttattt tttatttatg tttatgatta
    13321 ttatgtgtgt tgaatattta gataaatttt tatttattat ttttatttag atttttgttt
    13381 agttttttat ttggttgata tattaggaat aaaaattagt atagtttatt actagttaga
    13441 tg
//
LOCUS       DAYAOX010000105        13248 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.105, whole genome shotgun sequence.
ACCESSION   DAYAOX010000105 DAYAOX010000000
VERSION     DAYAOX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.105"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            87..323
                     /locus_tag="ACGI57_002271"
     CDS             87..323
                     /locus_tag="ACGI57_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HFG9717347.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            336..1679
                     /gene="yqfD"
                     /locus_tag="ACGI57_002272"
     CDS             336..1679
                     /gene="yqfD"
                     /locus_tag="ACGI57_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HFG9717348.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            1618..2733
                     /locus_tag="ACGI57_002273"
     CDS             1618..2733
                     /locus_tag="ACGI57_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFG9717349.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            2790..3251
                     /gene="ybeY"
                     /locus_tag="ACGI57_002274"
     CDS             2790..3251
                     /gene="ybeY"
                     /locus_tag="ACGI57_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFG9717350.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            3257..3976
                     /locus_tag="ACGI57_002275"
     CDS             3257..3976
                     /locus_tag="ACGI57_002275"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFG9717351.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            4095..4496
                     /locus_tag="ACGI57_002276"
     CDS             4095..4496
                     /locus_tag="ACGI57_002276"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFG9717352.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            4560..5453
                     /gene="era"
                     /locus_tag="ACGI57_002277"
     CDS             4560..5453
                     /gene="era"
                     /locus_tag="ACGI57_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFG9717353.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            5465..6844
                     /gene="mgtE"
                     /locus_tag="ACGI57_002278"
     CDS             5465..6844
                     /gene="mgtE"
                     /locus_tag="ACGI57_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFG9717354.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            6917..7072
                     /locus_tag="ACGI57_002279"
     CDS             6917..7072
                     /locus_tag="ACGI57_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HFG9717355.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            7038..7811
                     /gene="recO"
                     /locus_tag="ACGI57_002280"
     CDS             7038..7811
                     /gene="recO"
                     /locus_tag="ACGI57_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFG9717356.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            7804..8403
                     /locus_tag="ACGI57_002281"
     CDS             7804..8403
                     /locus_tag="ACGI57_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HFG9717357.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            8563..9441
                     /gene="glyQ"
                     /locus_tag="ACGI57_002282"
     CDS             8563..9441
                     /gene="glyQ"
                     /locus_tag="ACGI57_002282"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFG9717358.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            9441..11507
                     /gene="glyS"
                     /locus_tag="ACGI57_002283"
     CDS             9441..11507
                     /gene="glyS"
                     /locus_tag="ACGI57_002283"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFG9717359.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(11673..13220)
                     /locus_tag="ACGI57_002284"
     CDS             complement(11673..13220)
                     /locus_tag="ACGI57_002284"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HFG9717360.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEKLFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNKEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
BASE COUNT         5223 a         1444 c         2373 g         4208 t
ORIGIN      
        1 aaaattttaa aactaatctg ttacaacatc aataaagtgc ataaatacct atttcaagca
       61 taaaattaat taagggggta atgcttatgt tagaaatatc cactgattta caagtaaatc
      121 aaccagttat tactgttaca agtaatacgt ttattagtat agaaaattat ctatcaatat
      181 tagaatatga agttgattta ataagaataa agactaaagt taaaacaatt aaaatatctg
      241 gggataaact ttcacttaag tatattactg atagtgagat aggtataaaa gggattatct
      301 acaatgttga gtatgttgat taggaggaga tacacttgat aagttttata aggggatact
      361 atgttatagt tgtggaagga gtaggactcg aacaattttt aaatcatctt ataagaaatg
      421 ggataagtgt ttacaatgta actagaataa aaaatactaa aatggagttt catatagata
      481 gacaagatat aaaagacttt aagaatgttt atagaggaag taagtttgat ataaaggtaa
      541 agcaaaaaac tggggtccct tttattatca aaagagttta taagcacaaa ggtatgtgga
      601 tttgtgctct tgtttcttta tttttattga tgtctacatc acagtttgtg acagatgtat
      661 atatacaatc tcctgaaggt ataaaaaaag aagctcttag aaatgagctt tataaggttg
      721 gagtaaggcc aggagtctat aagaagagta ttgatagaaa agaagttaga gaccatatga
      781 tgtcaaaatt taatgatgtg gcatatttat ctatcaatgt aaaaggaaca aatatatttg
      841 ttactgttac aaaaaaggca gaatccctaa aatctacaga ccaatcaaat tattgtaatg
      901 taattgcttt aaaaaatggt ataatagaaa aggtaatccc tagaagtggt aaatcagtta
      961 ttaaatctgg agacatagtc caaaagggag atgtcttatt aaatggagct aataccaagt
     1021 caataccaga agtatgggca agtacttttt atgagtccac aaaaaaggca agttatgtag
     1081 atactgtaaa taaaaaaact ggagaaaaga aaaatatata tacactaagt ttttatgata
     1141 aagagtttac aatgagaaaa aatataaaat ataaagatta tgttgttgaa aataaagaaa
     1201 aaaaactatc aataggaaac tatacttttc caataaagat aaaaacgagt actttttatg
     1261 aaacaaaaaa ggttgaagta aaaaggaata aagaagagtt aaaaaaagaa ttatctgaaa
     1321 aagctttaaa agagttagaa tacataatcc cagcttcggc tagaataata gatgtaaaac
     1381 ataactttaa ggttaataaa aatatgttag aatatttaat aacagttcaa actagtgaaa
     1441 atattgctaa aatttatcct cttagtaagt cagaagcaga aaggtttata aaggaagaaa
     1501 gtaaaccaga tgaaggtgaa gaggaagttc cttccaatcc agaaaaaaga ccattaaatg
     1561 atataagaaa tgagtttgat gaggataata aggataataa agaccaaaac aattctgatg
     1621 aaaataatag taatcaaaat agtaataata gtcaaaataa taatagcaat aacaactaat
     1681 aaaattaaac ttaaaatctt aggaggacta attcaattga taatacaaaa gaaatttaca
     1741 atagcagatg gtaactttga aagagagtta tttggcaact ttgatgaaaa tgtaaaatta
     1801 atagagaaga ctttaaatat agatgtaata cttagagaag gcaatataat tttaattgga
     1861 gaagaaaaaa atgtagattc tgcattaaaa cttatgaatg aattacacca aactgtatca
     1921 aatggtaagc atttagataa gcagagtatt tcttattcat tatctctttt gcttgaagga
     1981 agtgaacaaa aaataaaaga acttgagggg acaatcgtca taactcaaag gggaaaagct
     2041 gttcaaccta aaactttagg tcaaaaagaa tatataaaac ttatagaaaa taatgatata
     2101 acttttggtg ttggacctgc tggaacagga aagacttatc ttgcggttgc tatggcagta
     2161 aaagctttta aaagagatga agttagtaga attatactta caagaccagc tgtggaagca
     2221 ggagagagtc taggtttttt accaggagat ttaaaggaca aagttgaccc atatttaaga
     2281 ccactatatg atgccttatt tgaaatgttg ggtgctgata aatttaataa gtatttagaa
     2341 agaggtacta ttgaggttgc accacttgca tttatgagag gtagaacact tgataattca
     2401 tttataatat tggatgaagc tcaaaatact acttctgaac aaatgaaaat gtttttaact
     2461 agacttggat ttggctctaa agctgtagtt acaggagatg taactcaaac agatttacca
     2521 caaaataaga agagtggtct aatacaagct acagagattc ttaaaggtgt tccaggtatt
     2581 gggtctataa tgctaacaga tagagatgtt gtaagacatg agttggtaca gagaataata
     2641 agagcttatg agaagcacga caaaagagaa gaatttaaaa aagaagaacg taaaaaagcg
     2701 aaaatgcagg aaaagacttt caaaagaaag taaaagtgta gtaatataga tatatagcat
     2761 tattaatgaa taaaaagtag gtgtagtgaa tggatttgat actagatgat agacaagata
     2821 aattagaagt aagtgaggag ttaattgaaa agataaagga tataatcata gaatgtttag
     2881 attatgaagg atatgatgat aattatgaag tgagtctttc ttttgtagat aataaagaga
     2941 ttcatgaatt aaatagagaa tatagaggag ttgatagagt gactgatgtt ttatcatttc
     3001 ctcttttaag tgatgatttt gaagatgttg agttagaaga agaatcccta ggtgacatag
     3061 tagtatctct tgaaagagcc ttagaacaga gtatagagta taaccatagt tttgaaagag
     3121 aagtatgttt tttaatatgt catagtatgt ttcatctttt aggttatgac cacgatacag
     3181 atgaaaatac aaaagaaatg agagaaaaag aagaacacat attaaataag ttaaacataa
     3241 caagggagta gtttttatga aaccagaaaa aactcgtcaa ggtataatta aagcatttaa
     3301 tgcagctata gaagggatat tgtatacatt taaatttgaa agaaatatga aaatacatta
     3361 tttaggttca gttgctgttc ttataataag tttgtttttt aacttttcaa aattagaaat
     3421 gataatgtta cttatgtcca tatgtttagt tgtagttgct gaaatgttta atacagctat
     3481 agaaaaagct gttgatttag taactgatga atatcatgtt cttgctaaga tagcaaaaga
     3541 tgttgcagct ggaggcgttc tagtggcagc actaaattca gtagtagttg gatatatttt
     3601 gttttatgat aaattgacag atatatctgg aatattaatt tataaaataa gagaatcaga
     3661 attacatata accttgatat gtatattatt ggttcttata gctgttgtag ttgtaaaagc
     3721 attgacttct acaggtactc ctctaaaagg aggaatgcca agtggtcatg ctgccttagc
     3781 atttgctata gcaactgcaa ttacacttat gactgaaaga gttgttgcct ctactcttgc
     3841 atatataatg gctgtattag ttgcccaaag tagaattgaa ggaaaaatac atacattttg
     3901 ggagactatt gctggagctt tgttaggtgt gttgatagct atattagtat ttcagcttgg
     3961 aatgttctat aattaaaata aatgtacata aaatatttaa aataaaacaa atctgtgcta
     4021 attaaatttt tatgatataa tgtagagtat taaataaaaa taacttttaa ttattgtata
     4081 caggaggagt tgttgtggat aacatagaat tgttaaggtt ggcagaagat gccagacaac
     4141 attcttatgc tccttactca ggttttagag taggtgcagc tcttttaact aagagtggta
     4201 aagtttatac tggatgtaat atagaatgtg catcattagg tggaacaaat tgtgcagaaa
     4261 gaacagctat ttttaaagca atatcagagg gtgataagga tatctataaa atagctatag
     4321 caagtgataa ctcagaaaat aatgagcaaa catatccatg tggaatatgc aggcaggtaa
     4381 ttatagagtt tggttctgat ataaaaataa taacgggata tactaaagga gaggtgtttg
     4441 agcatagtat aaaggatttg ttgccaaact atttctctgg aaatgattta aaataaattt
     4501 aaaagtataa ataaatttaa gtaattaaaa caagaagata aaaagttagg agacagttaa
     4561 tgttcaaatc aggttttgtc agtatagtgg gtagaccaaa tgtaggaaaa tctactttaa
     4621 tgaataatgt agttggagaa aaaatagcta taatgagtga taagcctcaa actacaagaa
     4681 atacaataca agcagtatat acagatgaag aaatgcagat agtattctta gatacaccag
     4741 gtattcacaa accaaaaaat aaattaggtg aatttatggt aaaggcagca acagaagctt
     4801 ttaaaaatgt agatttaata ttatttgtag ttgatgattc taaaaaaata ggtcctggag
     4861 atagaaagat aatagaggat ttaagaagtg ttaagacacc aataatttta gttgtaaaca
     4921 aaatagacca actagaccaa aaagatgagc tatttgatat aatcaaaatg tatgatagag
     4981 aaggtatttt taaagaaata gtaccaatat cagcattaaa aggaaagaat acggatacct
     5041 taataaaagt tatacaaaat tatttagaag aagggccaaa gtattttcca gattatatga
     5101 ttacagacca gccagaaaga gtattaatag cagaacttat aagagaaaaa gttcttcact
     5161 atcttaatga tgaaatacct catggtgtag ctgttgaaat tgaaaaaatg aaagcgagaa
     5221 atgataagga aatagtagat gtatctgctg taatatactg tgaaagagat tcacataagg
     5281 gaataataat aggaaaaaat ggtaggaaac taaaaggaat aggtaagtca gcaagacaag
     5341 atatagaatt gcttttaggt tcacaaataa atcttcaatt atgggttaaa gtcaaggaaa
     5401 attggagaaa tttacaaaat tatataaata attttggata taatgataaa taaaggtggg
     5461 ttatatggat aggaaactgg atgcttccca agatttatta tatgaagtaa aatcattaat
     5521 tgataataat aaagtattgg agttaagaga attaatagag gaatatcata taattgatat
     5581 attcgatata atggaaaatt tagaggaaga catgaaaatt caattatttg aagtacttcc
     5641 tttagatatg gcatcttcta tattagaaga aggtagtgta gagtttttta ttagtatttt
     5701 atctaaactg gatgtagaac actcgaaaaa tatacttgaa cttatgtctc ttggagatat
     5761 ggcagacaag ttgagtgaac ttgaagaaga agaaagagaa cacataataa atcttttgaa
     5821 tcaagagaat gccgattatg taaaagagtt actattctat gatgaagatt ctgctggtgg
     5881 aacaatgacc actggatata tttctataaa taaagatatg acagctcttg aggctataga
     5941 ccatatgaga gaagaagctg aagaagctga aactatttat tatatatatg tggttgatga
     6001 tgaagaaaaa ctagttggag tattgtcatt aagagaatta ataatagcta gagacgcaaa
     6061 tatagtagaa gatttgatga gtgaaaatat aatctctgtt tatgtagatg aagatagaga
     6121 agaagcagta agattagtat ctaaatacaa tttaatagca attccagtag tagacagaca
     6181 agaaaaatta aaaggaataa taacagtaga tgatataatt gatgtaatgg aagaagaagc
     6241 aactgaagat atgtataaat ttgcaggaag ttcagaacat gaaagagaag ttgcggaaaa
     6301 agaaaaccct acattgagag agcaaataat atcagcactt agaggacgat taccttggtt
     6361 aattattacc ttagttggag ggttgttggc ttcgttaata ctttctaact tagactatat
     6421 aatgaatcct gtatatgctc cattagtatt ttttatacct gtagtaattg gtatgggtgg
     6481 aaatattgga actcaatcat catctgttac agtaataaca ttgtctaata aggatttaaa
     6541 ttttagcaat gtggttagag aaggtattgt aggtattatt acaggtcttt tgtgtagtat
     6601 aataacaggt attgtaattt actttgttat gagaaagtta gacattgtat taattgtttc
     6661 aatatcatta tttataaata tggttttagg tgcaacaata ggtgcgttta tgcctgtttt
     6721 attaaaaaaa atggatgcag acccatctac agtttcatca ccaattatat ctacagctct
     6781 tgatataact ggtatagcag tttactttat aataacaacg gcattattgt caaaaattgt
     6841 ttaataactt tatgtatgat ttatcctctt cttactaaaa atataagtat aaatgtaaga
     6901 agggggtttt ttaaaaatga acaatatcag ttgggaagtt tttaaaaaaa caggtagtat
     6961 agatgcatat ttatatttct gtgattgcaa aaacatacat gaagaaattg aagaagtaag
     7021 ggagaaaaaa gaagacgatg ataatcctga acacccaggg gatagtactt aaagctataa
     7081 ggtataagga aagtgatata atacttacac tatttacaag aaagcttgga aaagtatctg
     7141 caatagcaaa aggagctaaa aaaaataaga gctctttgct ttcatcatca cagttatttt
     7201 cttatagcaa ttttacctta aaaaaacaag gtaatatgta caaggttact caaagtgaaa
     7261 taataaaaag tttttataat atatcctatg atatagaggc attttcttat gctacatata
     7321 ttacaaaatt agttgaaaat tctatacttg aaaatcagac taataatagg ctatttatat
     7381 tacttgcgca gactctgtat ttgtatacac aggataatac agacaatagg tttataacag
     7441 cagcattcga gttaaaattt ttagactata taggatttaa acctatagta aataaatgca
     7501 ttaattgtga atgtaaaatt ttacaaaatt ctgtgtttaa tatctacgaa ggtggtatac
     7561 tatgtagtaa gtgtagtaaa ctttttgata ataatataaa attagactta acaactataa
     7621 gtcttatgga atatgtatta agaaatgata ttttaacatg tagtaaagct aaagtatcaa
     7681 aatatataac acatgaactt gagaatattc tagacaagta tttgaaagtt tatgttgata
     7741 atattaattt taaatcatta cacgtattgc aaagtgtaaa aaataataag ggagtggata
     7801 atgatgagta gtaatataac tattgaaatg gttgattcag tctttgaaag agttccagga
     7861 gcaacttatg cagaagcaaa agaagcatta gtaatgtgca atggagatgt aattgaggca
     7921 gtaatatatt tagaaaataa aaaaaatact tgtaattgta aagcaaaatc tgctaaagaa
     7981 actatggaag aagtatttgg caaagatgga gaaaatataa agtttcaatt aaaagagctt
     8041 cttagaaaaa gtagtgtagt gcgaataata gttgaaaaag atggaaaaac tatgatgaac
     8101 attcctttaa cagttggagt tgttggatta gcttttgtac cattagctac tttagttgga
     8161 ttatcagctg ctgttataag taaatatcgt ataaaagttc aaaatgaaga agatggagaa
     8221 attgtagatt taggtgaatt gaatgaagaa aaacttcacg ttttgaagaa tatgataact
     8281 aatgcagcaa aagatgtgaa ggatgtagta gttgacaaaa aagaagatga caaagatgta
     8341 actgctgacc ttatgaaaga agaatctgaa aagaataaag aagacgaaga cgaaaataaa
     8401 tagatataaa gtgactttta aaaaatgaag agaagataat tataagaatt atcactaaca
     8461 ttgacaaatt ttatagaatt tgatattcta aaagttgtag aagaactatt aaaatagcct
     8521 ttcgtgtaga cgagaggctt tattgttata ggagggattt ttatgaattt tcaagagatg
     8581 atactggcgt tgcaaaaata ctggtcaaag caaggttgta tcatgatgca gccttatgat
     8641 attgaaaaag gtgcaggaac tatgaatcct aatacttttt taagatcatt aggacctgag
     8701 ccttggcaag tatgctatgt agagccttca agaagaccag cagatggaag atatggtgaa
     8761 aacccaaaca gactatatca acatcatcaa ttccaagtta tactaaaacc ttctccagat
     8821 aatatacaag aattatattt ggaaagttta aaagagattg gaattgaccc aagtgagcat
     8881 gacataagat ttgttgaaga taactgggaa gctgctacag ttggtgcttg gggacttgga
     8941 tgggaagttt ggctagatgg tatggaaata actcaattta cgtattttca acaagtaggt
     9001 aatattgaat gtgagcttga gacaggagaa attacatatg gattagaaag attagctatg
     9061 tacattcaag aagttgatag tgtttatgat ttaaaatgga atgataaaat aacttatggt
     9121 gaagtattta ataaagctga gtatgaaaac tcaatgtacg catttgaatt atgtgatgca
     9181 gatatgttgt ttaatctgtt tgatatatat gaaaaagaag cattaagact tatggaaaat
     9241 ggccttgtaa taccttcata tgactatgtg ttaaaatgct ctcatgcatt taatacattg
     9301 gatgcaagag gtgcaatagg agttagtcaa agagcttcat ttataggtag agttagaaat
     9361 atggcaaaga ctgttgcaga aacttttgta aaacaaagag aagaaatggg attccctctt
     9421 ttaaaggacg gtgacaaata atgaaaaatt atcttttatt tgaaatcggt gttgaagaaa
     9481 tgccatcaag atttgtaggc agtacactag aacaattaaa aaacaatctt agtaaattat
     9541 ttgatgaaaa tagaatagct tttgataaaa taaatgcgta tgggacacca agaagactta
     9601 ctttagtagt cgaaggattg agtgatagac aaaatgattt agaagaggaa ataaagggtc
     9661 ctgctaagaa aattgctgtt gatgcagaaa ataatttaac aaaaccagca ttaggattta
     9721 taaaaagcaa aggattaagc gaatctgaca tatattttaa acaagttgga aaagaagaat
     9781 atatatttgc gactataaag caagatggaa aagaaacttc agaagtacta aaagacatat
     9841 taccagagac tataaaatct gtagtattcc caaaagctat gcgttgggga ggaaaaaatc
     9901 ttaaatttgc aagacctata agatggatag ttgcattgtt aaatgataaa gtattagagt
     9961 ttgatttaga aggaataata tcttctaatg taacaaaagg tcatagattt ttaggtgaaa
    10021 gtacaataga agttgattct atagaagatt attttgaaaa attagaaaaa aattatatag
    10081 ttttagacca aaacaagaga aaagaaatga taagaaaaca atgtatagaa gtggcaaatt
    10141 ctttaggtgg agaagttgaa tttgatgaag atttacttga agaggtaact tacttagttg
    10201 aatatccaac tgctttttat ggagaatttg atgtggatta tataaaacta ccaaaagaag
    10261 ttgttataac tcctatgaaa caacatcaaa gatattttcc agtacttaaa gaaggaaaac
    10321 tgttacctaa ctttatagct gttagaaatg gtgattctta cagaatagac aatgttaaag
    10381 ctggaaatga aaaagtttta gaagcaagac ttgcagatgc tttatttttc tatagagaag
    10441 atactaagag aaacttagaa agctacatag aaaaattaaa aactgttgta ttccaagtaa
    10501 aactaggaac tatttacgac aaaactttaa gacttgaaaa attaagtgct gatatacttg
    10561 atagtttagg tttagtagat gaaaaggcag atactataag ggctgctaaa ttatctaagg
    10621 cagatttagt tactggaatg gtaggagaat ttgatgaatt acaaggtttt atgggaaaag
    10681 aatacgcaaa agttggagga gaaaatgatg ttgtaagtga agctattttt gaacattatc
    10741 ttccaagatt tgcaggagat atacttccta agactaatgc aggagttgcc ttatcaatag
    10801 ctgataagtt ggattcaata gcaggtttct ttgctatagg aatacaacca acaggttctc
    10861 aagacccata tgctttaaga agacaagctt taggtatact tagtatactt atggacagaa
    10921 aagttgctgt ggatataaaa gtacttattg aacatgcttt agacaattat tctgaattag
    10981 attttaataa agaagaagtt gttgagcaaa taatgaattt cttcaatgag agagtaaaaa
    11041 atctatttaa agatttagga ataagatatg atgttgttga tgctgtttta agttctgata
    11101 taaatgatgt ttctgatatg catatgagag ctttagagct taataattgg ctagaaaaag
    11161 atgaattagt ggagatgctt actgcattta atagagtttc tactctagct caaaaagcag
    11221 aaagtgataa aattgatgaa tctttattaa aagaagatgc tgaaattaaa ctttacaatg
    11281 aatttaaaca agtaaaggta aaagttaatg aattattaaa ggataagaaa tatggtattg
    11341 ctttagatga atttgcatct ttaagaccag tagttgataa tttatttgat acagttatgg
    11401 ttatggataa tgatgaagct attaagaaca atagacttgc tttacttaaa caagtatatg
    11461 atactatgct aggaatttgt gatttatcta aaattgttta taaataaaat ttaaactatt
    11521 tataagaaat aaggctgtct caaaatagag atagtcttat ttttcattgt tgagatgatg
    11581 aaaatatatt tgatttcata gaaaaagagt gtctcataaa ctaaaaagtt cattttgaga
    11641 cacccatgct ttttgtaagt aaatataata aattatattt tatacttatc agttattttg
    11701 tttaaaatat tatcatttga atttacccat tcataaaaat tgcctgatga tgcgtccgat
    11761 attttataaa gcatttcagg aatatcacta gctttaaaat ctccaagtga ttttcccaat
    11821 ctagtcttac ctatttcaaa actgcctcca acaaataatt caaatgcatc tgtagatata
    11881 ccatcaacat tcttcttttt accacataaa cctatgctac caacttgatg aacaccacat
    11941 gaatttaaac atcctgatat atataactta ggtgctttat ttaaaatttc tttattattt
    12001 tgtaatctaa aataatctat gatttcatgt aacattttct gactattttg tatgcccatt
    12061 tgacaaattg gaacacctat gcaagatata ctttgttcta attgagaatt acaactaata
    12121 gttttactta tttctaatat tctttttgct tcatttccat ttaaatttaa aatatataat
    12181 ccctctgtca ttcctagtct aatcatagga ttttcaatat tatctaattc tttaagtaaa
    12241 gtacttaaat cctttgttaa caaaatacct ccaactggat gtatatatac agtgtataaa
    12301 ccatcctgtt tttgaggtat tagtctagaa tcctgtatat ctattttaac cccaggttta
    12361 gagtaatcta tttcttcaat attgagttca agactaccat tttctttttc taatttatag
    12421 tattctttaa atttttctaa gaataatttt tcaccaagtg attcgaccat atatctaacc
    12481 ctagctctgt ttttattttc ataatttcca taatccataa agaatttaat cataccttca
    12541 acacaatata aggcatcttt cggttttata agttcatcaa gttctaaagc aactttgggt
    12601 tgtttaccta gacctccacc taagaaaact ctgaaatatg gttcatcatt tttcaaagta
    12661 gcaacaaatc ctaagtcttg tatactacaa tgagcagtat ctgtgtaaca attagaataa
    12721 gaaactttta attttctagg taaatggtat gtagtagcat ttttgataaa atatttatca
    12781 gtagcaactg catatggagt aacatcaaag acttcactag ggtcaactcc agataatgga
    12841 gataatccga catttctagg ataattacca ccaccacctc tagtgaatat atcatttttt
    12901 attccttctt ccatgatatc aacaattgaa tttatagata aatcgtgtaa ttgtatagct
    12961 tgtctagttg ttaagtgtat cttatctaag ttgtgtttaa tagctatttc atagatttta
    13021 ttaagttgat actgagataa aactccagca gatactctaa gtcttatcat aaaagatttt
    13081 ttgtctcttt gagcataaac accatatcca ccagaaaatc ccttatattg cattttattt
    13141 atttctccat ttaaaaattt tgaagaatat tctttaaaat taggtagttc atttaacaat
    13201 atctttttaa aattatccat tatttcacct cattgttagt cttgtata
//
LOCUS       DAYAOX010000106        12765 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.106, whole genome shotgun sequence.
ACCESSION   DAYAOX010000106 DAYAOX010000000
VERSION     DAYAOX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.106"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(208..1785)
                     /locus_tag="ACGI57_002285"
     CDS             complement(208..1785)
                     /locus_tag="ACGI57_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HFG9717361.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
     gene            complement(2082..4493)
                     /locus_tag="ACGI57_002286"
     CDS             complement(2082..4493)
                     /locus_tag="ACGI57_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9717362.1"
                     /translation="MRKYKLKKLSKLLVLLTVCFLIVSTIPVSAEKNKILTGVETAEY
                     SESYLQYLEDVKNGDIAKYNGVIPTPYEMEGTTLKTNVRSSLPASYKSSVSYDPRKLD
                     LTTPAKDQGKLNTCWAFSGMSTLEAYLKLKGYGTYDLSEEHFRWWSTGGIHGWNLTDM
                     TGSSNVTAIGYLTAWAGPKLEKDIPYNFKSEDEGATRPQNMDTAPTQFNVTDVVRISK
                     DKTSVKNAIMQYGAVTSGYAHYSAYLSDDENSYNCNDKSEPLNHSVSIVGWDDNYSKD
                     KFKPSVRPESNGAWLVKSSWGEFNSEKGFFWISYEDKTLLKDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            4668..5084
                     /locus_tag="ACGI57_002287"
     CDS             4668..5084
                     /locus_tag="ACGI57_002287"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFG9717363.1"
                     /translation="MEENFLILKKHLETILYLFFGALTVLINIITYLFFTRIINFTFL
                     LSNSLSWSIAVLFSYITNKFFVFKSEKTEFLFLLKEFVSFLSCRLFSGFIEILLMYLM
                     IDLFSLNDILVKIGTNIIVIILNFVFSKVVVFKHRN"
     gene            complement(5149..7266)
                     /locus_tag="ACGI57_002288"
     CDS             complement(5149..7266)
                     /locus_tag="ACGI57_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9717364.1"
                     /translation="MQISKKIISLLTAAFLSIVLPSNVVNASNKDSLIGSGRWETAIK
                     ISQNGWLRSDNAVLVNDNSIADALSATPFAKLKDAPILLSGSKELDFRTKSELKRLGV
                     KNVFLVGGSNALGVNVENELKSEKITVERIFGNDRYETSLKLAEKLNNEKDISDIIVV
                     NGEKGLADAVSVGSIAAQNYMPIILSDSNLGVEVANKFIKAQNIKNSYIIGGLNSISN
                     EIEKNLPNSKRISGNNRNETNANVIKEFYKETDLRNAYITKDGMKKQSDLIDSLSVGV
                     LAAKNNSPVVLVGNKIDSIQKDVLNTKIFSTVTQVGGIGNENVYKDILDMQEETKYTV
                     ETIEELDSALKKVDANDIIKFRPDKKISESFKLETEKAIKVDLYGEYSQSITINMPNG
                     ELNNTGDISKTLILKDIKDGTLTNSGYINQMDISDDNGCKIENENNGQIWIVTIFSSA
                     KDVNIRNDGTITKIDNDSSSTNIRNSGKIDTVSGNKEPGISGNKPNTNNTDKDDDSDT
                     AAGLSPKISACSPLENNQIMLTVPQKPKNSNYKIYYRVLSYKPSAMRVGDRININDWD
                     LVEDTEPLKVNAKNGSYIEVVEIDYTRKKVSRWGRAGATDDGFKIVANGIKSTVSVDE
                     NSKVTISVTGYNGNKVYYRIKTTKPEAMNVGDEIDSSWKSTSNSDIILEGNKVNGLYV
                     ELASGENNKVEKWGATDKIEIVS"
     gene            complement(7297..8004)
                     /locus_tag="ACGI57_002289"
     CDS             complement(7297..8004)
                     /locus_tag="ACGI57_002289"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFG9717365.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAKEIILKYLEEYPEAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDDLELNKTQYVHAP"
     gene            complement(8111..9982)
                     /locus_tag="ACGI57_002290"
     CDS             complement(8111..9982)
                     /locus_tag="ACGI57_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HFG9717366.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRGLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            complement(10326..12671)
                     /gene="secA"
                     /locus_tag="ACGI57_002291"
     CDS             complement(10326..12671)
                     /gene="secA"
                     /locus_tag="ACGI57_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFG9717367.1"
                     /translation="MSVLDTILDKADEQEIRRLNVIVDKIESLEKDIENMSDDELKKM
                     TDLFRDRLKKGETLDSLLPEAFAVAREASKRVLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVVTVNDYLAQRDKELMQPVYEFLGMSVGVIIADLI
                     PEVRKVQYNCDITYGTNNEFGFDYLKDNMVTKKFDKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGEGDEDSKLYELANSFIKTIKEEDYELDRKDKTVALKESGIDKAESFFGIKN
                     LTDIENIELYHHINQALRGHKLMEKDVDYVVVNQEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKDESKTMATITFQNFFRLYNKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPIIRADLHDKVFRTEKEKYQAVVEEVIRIHKTKQPILIGTVSVEKSEMLSGMLKK
                     EGIKHQVLNAKENDKEALIVSKAGKLGSVTIATNMAGRGTDIALGAGDKEEESRVKEL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKSIEKLMKR
                     PNSNKNNSIESKSLTKAIERAQKGVEGKNFELRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQVDIQNMVKDIIKEAAAEYLTKRKRDYYGYFKHLYNTFMPADTLLIPDLDNKS
                     IEDIIDSTYEISKRVYDLKKMVVGLDKITEMEKIVLLKVVDQYWVEHIDAMEQLKQYV
                     GFKSYAQKDPFKEYAMEGYEMFESLNKNIRETTIQYLYKFN"
BASE COUNT         3748 a         2344 c         1368 g         5305 t
ORIGIN      
        1 agattatttt tctttattct agttttgtca atacttttat ttattttttt gttacattca
       61 attccaatcc ccatgttacc tccccaaagc ttattcaaat atatactaaa ctacatattt
      121 atctataaat ctatttgtat ataaacctgt ttaactacat aactttagaa caattacaac
      181 aaacacttca cttaaatata tatattctta gctaagaaat ttctttagaa atccaagctt
      241 atctagtata tcttgtattt tattgacttt ttgacttata gaatctatag ctttttcaat
      301 tgaatctact ttagcctcta aatcttcaac ctgattaact tttttatcta tagtaccatt
      361 attagttata tcactattag atgaattatc tataagtttt cctatatatc catcattaat
      421 caattttgta tcattagcag aagctaatat catcaacgta tctatatctg aatttctagt
      481 attttctata actctaccat ttttgtcacg aactgacaat atagtgattg tatcatggtt
      541 attaaatcca ccttctccaa tatttctaac tataacattt accaaagtag catagttatt
      601 cacttcacca ttaggcatat ctactgtaag agtctttgaa caatcaccct ttatattcac
      661 agttattggt ttatttgtta ccattctaaa agcttcattt acagtattct cttttggttt
      721 aaagtttata atatcattag gacttgctat atttatcata tcagttaatt cctcaacagt
      781 ttttgcttca aacacagttt tttcttgtag acttttaatt tcatcaaaag cttcttcatt
      841 tccatttcca cctacctgtg ttatagtatt taatttttta gtactaagta tgtccctttg
      901 ttttgtgttt aatttattac caaccaaaac aacaggagac tcattttttg cagccaatac
      961 tccaacagct aacgagtcta ttagttgatt ctcattttta ctaccatcct tagttacata
     1021 taaattactt aaatttgtat cagtgtaaaa cttttctatg accttagcat tagtctcatt
     1081 tctatctttc ccactcactc tttctgcatt aggaagactc ttttctacag ctcttgaaac
     1141 agaatttgta cctccaatta catatgcttt tataactttc tcatccctta taaatttatc
     1201 aaatgcttct actccgtcct taggactaga caaaattata ggcatcttat tttgagctgc
     1261 tggtgcccca acgctaacag catcactcaa tcctttttca ccatttacta cagcaacttc
     1321 cttaatatct tttatatcct taagcttatt agccaaaatc agtgaagttt catatctatc
     1381 cttaccatta attctttcta cattaagtcc attacttttt aacttgttct ctatatcttt
     1441 attaagtaca gcagtacctc ctatcaaata aatatcttta gctcctagac gttttatctc
     1501 tttttcagtc ctatcatcta gcttatcact ttctgtcaaa agtattggtg ctcctttagc
     1561 ctttgcaaat ggtgtagctg aaagtgcatc agctaatgaa ttgtcattaa ctagcactac
     1621 attaggacta gaactgtatg ccttttgact tatctttaca gcagtttcat atctaccatt
     1681 acctattaat ttttcctctg taagtgcata tgatggtatt atagaaatgc tacttaaagt
     1741 aagcgctatt gtcagtattg ttaaaatagt ttttggtgct ttcattatta ttccccctta
     1801 ttttgtttaa aatcttacta tatttatatt ataaaataac tttacactat tttacagttt
     1861 aaaattatta catttttcat caataataag aaaactatct aaaaatatag acaatcctat
     1921 ttttcattat ttaaatgata aaaatataaa acaacaaaaa agagtgcctc atgaactaaa
     1981 aagttcattt tgagacaccc cgttttgtta ttttatattt ataaacttct attgaatatt
     2041 ttttaataat aaaatgtata tccttactat ctttccattc actattttcc taaaagagta
     2101 tttagttcat taaaagcatt ttcatttcca tttccaccaa ctctaatagg agtttctatt
     2161 ttcttagact taagtacatt tttttgactt tcatctaact tatttccaac taaaactact
     2221 ggtgaatcag ttttagcacc taatgctcct actgctagtc catctatcaa gtctccttga
     2281 ctctttgaac catctttaac aacaaaagca tatttcaaat ctgagctagg atagaattta
     2341 tctattatct tagcattagt ttcatttcta ttacttcctg caagtcttgt tggatttgaa
     2401 agcttacttt ctaaatttga tgataaagta gctgttccac caattatata agacttagtt
     2461 attttagatg aatttaaaaa ctcatcagca ccttgcaact cactcttttc attagtaagt
     2521 attattggca tattgttatc agcagctgct gcaccaacac ttattgcatc tgcaagtcca
     2581 tttacaccat ttgccactgc aacttgtgaa acagatttta tactatttaa cttttgagct
     2641 agagatatac tggtcttgta tctatcacta cctgaaattc tttctactga tatacccata
     2701 ttactaagtt gagtttgtac attcttgctt aaagaatttt ctccacctat tagataaact
     2761 tttttagttc ctaatctttg taactctgcc ttagtttttg aatctaagtt atcttttcct
     2821 gtcaaaagaa ttggagagtc aattgctgct gtaaatggtg tagctgttaa agcatccact
     2881 attgcatctc catttactaa tactgcattt tgtgaagaag tccatccttt ttgactaact
     2941 ttaactgctg tttcatatct atctgctcca gttaaagatt ctcctgaagt agcttgtcca
     3001 gaagatgttt ttgttatagc ttttataaca aagttacaag gagaaaattc actatatgta
     3061 tttatatctt caaatttatt gttttgaagt atgtagcttt cacctaagtt tgctttagcc
     3121 tcatatagat agtatccatc tatatcagtt tcatatgcta aagttgattt ttctctatta
     3181 ggatttgctg tgttgtctat aactactact attgctcctt taccttttgg taagctgtaa
     3241 gaattagtag gtacatttat gtatccagaa tatgatactg ttccacttgc aagttttgtc
     3301 attgaattgt tttgaggaac tccatttact actggtgcat aatatacttc atattttgct
     3361 cctacagaat ctgtttcaaa cataacagag tcaagttttt cagaatctct gctaaaatca
     3421 aatacatttg ctgctgttac tttatttgac attatcttgc taagaccagc atattcaagt
     3481 tggtacattt ttttatcgct atctggtttt gatactgatt tcattgcgta gttatctgta
     3541 tcttttaaaa gagttttgtc ttcataagaa atccagaaaa agcctttttc tgagttaaat
     3601 tctccccaac tactttttac taaccatgct ccatttgatt ctggtctaac acttggtttg
     3661 aacttatctt ttgaataatt atcatcccat cctacaattg aaacagaatg atttaaaggt
     3721 tcacttttat cattacaatt atacgaattt tcatcatcac ttaagtaggc tgaatagtgt
     3781 gcatatcctg atgtcactgc accatattgc attattgcat ttttaacaga agttttatct
     3841 ttactaattc ttacaacatc tgttacatta aattgtgtag gtgcagtatc catattttga
     3901 ggcctagttg ctccttcatc ttctgattta aaattatatg gaatgtcttt ttccagctta
     3961 ggtcctgccc aggctgtaag atatccaatt gcagttacat tcgaactacc tgtcatatca
     4021 gttaagttcc atccatgtat tcctccagtt gaccaccatc taaaatgctc ttctgaaaga
     4081 tcataagttc cataaccttt taatttcaaa tatgcttcta gtgttgacat tccagaaaaa
     4141 gcccaacatg tattaagttt tccttggtct tttgctggtg tagttaaatc taattttctt
     4201 ggatcatatg atacacttga tttatatgat gctggaagac tacttcttac attagtttta
     4261 agtgttgttc cttccatttc ataaggagtt ggtattactc cattatattt tgctatatct
     4321 ccatttttta catcttccaa gtattgaaga tatgattcag aatactctgc agtttctact
     4381 ccagtcagaa ttttattttt ttctgctgag acaggtattg ttgacacaat taaaaaacaa
     4441 actgtcaaca acaccagtag ctttgacaat ttttttaatt tatactttct catatttacc
     4501 cccttgtatg tatttaattt ttattatatc agatttctaa atctaggtca tattttattt
     4561 ttataaaaat aggaattata attatacata tataaaatac tattttatac tgcatattta
     4621 ttgattaata tgtggtatac taattacata ataatatata ccaaataatg gaggaaaatt
     4681 ttttgatatt aaaaaaacat ctagaaacaa tattatatct tttttttggt gctttaacag
     4741 ttttaataaa cattattact tacctatttt ttacaagaat tattaatttt acttttttat
     4801 tatcaaactc tctatcttgg tctatagcag ttttattttc atatataaca aacaaatttt
     4861 ttgtattcaa aagtgaaaaa actgagtttt tatttctatt aaaagaattt gtatcttttt
     4921 tatcttgtcg tttattttct ggttttatag aaatattact tatgtattta atgattgatt
     4981 tattttcttt aaatgatatc ttagtaaaaa taggcactaa tataattgtg ataattttaa
     5041 acttcgtctt cagcaaggta gtagttttta agcatagaaa ttaacattat tatatttatc
     5101 tttatgcaga ataaggagtt gcctaaaaaa aacaactcct tattagcttt aagaaacaat
     5161 ttctatttta tctgttgctc cccatttttc aactttatta ttttctcctg atgctaattc
     5221 tacataaagt ccatttacct tatttccttc taaaattata tcactattag aagtactttt
     5281 ccaactggag tctatttcat ctcctacatt catagcttct ggttttgtag tctttattct
     5341 atagtaaact ttattaccat tgtatccagt tacactaata gttactttag aattttcatc
     5401 aacagaaaca gttgatttta ttccatttgc tactatttta aaaccatcat ctgtcgcacc
     5461 agctcttccc catcttgaaa ctttctttct tgtataatca atttcaacaa cttctatata
     5521 tgatccattc ttagcattta cttttaatgg ttccgtatct tcaaccaagt cccaatcatt
     5581 tatgttaatt ctatcaccaa ccctcatagc tgatggttta tatgacaaaa ctctataata
     5641 aattttataa tttgaatttt taggtttctg aggtacagtt aacataattt gattattctc
     5701 aagaggagaa catgcactta tctttggaga taatcctgct gctgtatcac tatcatcatc
     5761 tttatctgta ttattagtgt taggtttatt gccacttatt cctggttctt tatttccact
     5821 aacagtatca atctttcccg aatttcttat attagtactt gaagaatcat tatctatctt
     5881 tgttatagtt ccatcatttc ttatatttac atcttttgca gaactaaata ttgttacaat
     5941 ccatatttgt ccattatttt cattttcaat cttgcaacca ttatcatcag aaatgtccat
     6001 ttgatttata tatcctgaat tagttaaagt tccatctttt atatctttaa gtatcaatgt
     6061 tttacttata tcacctgtat tattcaactc tccatttggc atatttatag ttattgactg
     6121 actatattct ccatataaat caacctttat agctttttca gtctctaatt taaaagattc
     6181 acttattttt ttatcaggtc taaactttat aatatcatta gcatccactt tctttaaagc
     6241 agaatccaat tcttcaattg tttcaactgt atatttagtt tcttcttgca tatccaatat
     6301 atctttatag acattttcat ttcctattcc accaacctga gtaactgtac taaatatctt
     6361 agtatttagt acatctttct gaatagaatc aattttattt cctactaata ccactggaga
     6421 attgtttttt gcagccaaca ctccaactga tagtgaatct attaaatcac tttgtttttt
     6481 cattccatcc ttggttatat atgcatttct taaatctgtt tctttataaa attcttttat
     6541 aacattagca tttgtttcat ttctattatt tcctgatatc ctcttagaat ttggtaaatt
     6601 tttttctatt tcattagaaa tggaatttaa tcctccaata atataagaat tttttatatt
     6661 ttgggctttt ataaatttat tagctacttc aactccaagg tttgaatctg aaagtataat
     6721 tggcatgtaa ttttgagctg ctattgaacc aacacttaca gcatctgcta atcccttttc
     6781 tccatttact acaattatat cagaaatatc tttttcatta ttcaattttt cagccaattt
     6841 taaggatgtt tcatatctat cattcccaaa aattctttca actgttattt tctctgattt
     6901 taattcattt tctacattta cacctagtgc atttgaacct cctactaaaa atacattttt
     6961 tactccaagc ctttttagtt ctgattttgt cctaaaatcc aattctttac ttccagatag
     7021 taaaataggt gcatctttca atttagcaaa tggagttgca gataatgcat ctgctattga
     7081 gttatcattc actaatacag cattatcaga tcttaaccaa ccgttttggc ttatttttat
     7141 tgctgtttcc catctacctg aacctattaa actgtcttta ttagatgcat ttaccacatt
     7201 actaggtaaa acaattgata aaaatgctgc tgttaataaa gatattattt tttttgatat
     7261 ttgcattaat tatctcctcc taatttaaat ataattttat ggagcatgta catactgtgt
     7321 tttatttaat tctaaatcat caaaatgaga ttttactgaa tggtatccta tagcataatg
     7381 tccactttca ggattccatt tcttatatgc attcatcgca cttaataact tctcctcttg
     7441 actaggtaat accttccaga cttctacatt tggattaact tttttaaaat ccttatagtc
     7501 atatggttct acataaaatc ttaaatcttt tatttttcct tctctatata actctagtgc
     7561 agcttctcct aatgctctat gatcttcatg tattccacta gctttaaacg gagtaacagt
     7621 ttttactttt gcttctggat attcttctaa gtatttaagt attatttcct ttgccttttc
     7681 tttgcttaac tcaccatcat gagctctatt atcttctata tgtatattac tttcttttac
     7741 acccattgct tcacagctat ccttaaattc ttcatctcta tcttttgaaa attcactttc
     7801 tgataaagag tatttatgaa catctttaat atgataagaa catgaccctc catcatccaa
     7861 aacttttctt attatagatt tactaccatc tgtgcacaat atgacatgtg tatcactttt
     7921 ttcaacatgt tcaattatag cacttcccat acttaaggtt tcatcatctt gatgtggtgt
     7981 aaaaaacaaa gttgcatcta ccactactac atcctcctac tttataaaat gttataattt
     8041 acaataaata gcataattat agaaaaagat tcctgtatta attaaggaat cttctccaca
     8101 gtataagtta ttaccaacct agtattttag ctatcttttg tattactgtc aattcaactc
     8161 cattacctat ttgagttact tctgcaccaa gatttaattt tttactaagt gcattctttt
     8221 gtgagtctgt tatatcagtt ttagctagaa gtattggacc tttacctgct gctagaggtc
     8281 ctgctgccaa tgcatctact agcataccat tatttccata tccatctttt gctatatata
     8341 gtttatctaa tttgccataa tatgtttcta taactttagc attagtatcg tgtcttgttt
     8401 ctccttcaac tctttgaact tttctatctt tatcaatatc tgcaagttga ttttcaattt
     8461 ggctagatac attattagag ccaccaacta tgccaatctc ttttccatcc atcaatttga
     8521 tagcatctgc tgatagatca ttccaaccat ttactattat aggagcctta agttcagcag
     8581 ctttagcagc tatactcata gcatcagcct ccccttttgc gcctactaca aatgcatctt
     8641 taaaagaccc catagctttt gctactgcta cagaagtcat atgtctatca tctccagcta
     8701 gtctttcaac attcttagtt actgattcta attgcttttt agctgtattt gatactgctg
     8761 attctccacc tactatatat attttagctg atgggctttt cttaattata tcttttatat
     8821 aatctaaagt tacttttggt atttcattat cagatgctaa taatataggt gcttttttag
     8881 attgagcaag tggtgtagct gctaatccgt caactagttt atttgaatta actataacta
     8941 tattgtcagt atattttgtt tgttttgcta tagccactgc agttgcatat ctatcatctc
     9001 cagctatcaa tttaaccttt gaattacctt ctaatgcagc tttggcatct tttaaatctt
     9061 tttcattggt actttctaca gtaagctctg ttgcttttcc agatgcatca gtatacttaa
     9121 tagtaaattt atattttcct gtttctgaat cttttgctga aatatcacta attgactttg
     9181 atgtaccatt tgctaccata ttacctttaa cagtcaatct atatccatca tataaatctt
     9241 gtggtgaaac tgtatttagg tcactatttt ttatagttat ttcggccaat tggacatcat
     9301 caatattttt agctcctgct acacctagtt tatcgatttt atttactcta aatccttctg
     9361 cagaagggtc taaatttgtt ccagttgaat caactggatt tgctgttaaa tcaacttttt
     9421 catcaccaat acctacatat atagttttag aagttactgt ttctgtttta tctcttaaag
     9481 ttataactaa cttatcttta gcactatcat atgaagcttt tacatctgca actttataaa
     9541 ttccattggt ttcagtttta gctttttctg ttatagaatt agcctcagcg gaaaggtctg
     9601 ctgaattttc atatttttca gttgcttctg caactacttc atttgcacta tttgttatat
     9661 gtcctttatc tgttatagtt acaataagtt ctttattttc tcccaaagca tttactaatt
     9721 tctctaattc atctatatta gttattattt ttaattttga taaagtctca ccaactttta
     9781 tttcatatat tggttgtcca acttgagaac ctcctgtata cttttgagac attaaacctc
     9841 taactttcga aactaattct gtagcctctt tcttagttat tgtttctttt tttacctgtg
     9901 tagtttctgc agcaaaaact ggagctactg atgtacttat cattgcagca gccataatta
     9961 cagaaagatt ttttttattc atggtatttt ccccctaaaa aattcaaatt tttattattt
    10021 tttccttatt taccaattat aatatatatt tgatattatt tcaataaatg agacttattg
    10081 taataattaa ccaaaagata taaacttttt cttatatttt atttttttaa tatttacgta
    10141 tacaaaaaag taacaaaatt gtaatttttt ttaataatcc agtttataac taatagactt
    10201 ttcatcttct ttatagtata atattaaatg gaagaaagaa atttcaagca tctctttttt
    10261 ccgtatttct cacatagaaa aagaggctga ttatcagcct ctttttccta tacagtttaa
    10321 atttcttaat taaatttata taaatactgt atagtcgtct ctcttatatt tttatttaat
    10381 gattcaaaca tctcataacc ttccatagca tattctttga aaggatcttt ttgagcatat
    10441 gatttaaatc ctacatattg ttttaattgc tccatagcat ctatatgttc tacccaatat
    10501 tgatcaacta cttttaaaag aacaattttt tccatttcgg ttattttgtc aaggcctact
    10561 accatctttt ttaaatcata aactcttttt gatatttcat aagtactatc aattatatcc
    10621 tcaatacttt tattatctaa gtcaggtatt agtaaggtat cagctggcat aaatgtatta
    10681 taaagatgct taaaatatcc atagtaatcc ctctttcttt ttgttaaata ttcagcagca
    10741 gcttctttta ttatatcttt aaccatgttt tgaatatcta cttgtatatc ttcatcattt
    10801 agaactttat ttctttcgtt atagattact tttctttgtt catttatagt atcatcatat
    10861 ttaagaacat ttttccttaa ttcaaaattt ttaccttcca cccctttttg agctctctct
    10921 attgcctttg taagagattt actttcaata gaattatttt tatttgaatt tggtcgtttc
    10981 attagtttct ctatactttt tccaccatac aatttgatta catcatcttc aagactaaca
    11041 aaaaaccttg atgttcctgg atcaccttga cgtccagaac gccctctaag ctgattatcg
    11101 attcttctag attcatgtct ctcagttcct attacataaa gtcctcctaa ttcctttact
    11161 ctactttctt cttctttatc acctgctcct aaagcaatat ctgttcctct acctgccata
    11221 ttagttgcaa tagttactga acccaactta ccagcctttg acactatcaa tgcttcttta
    11281 tcattttcct tagcatttag aacttgatgt tttattcctt ctttcttaag cataccagat
    11341 aacatttctg atttctcaac agaaacagta ccaataagaa ttggctgttt tgttttatgt
    11401 atccttatta cttcttctac aacagcttga tacttttctt tctctgttct aaacacttta
    11461 tcatgaagat ctgctcttat aattggtttg ttagtaggaa tttgtacaac atttaattta
    11521 taaattgatt caaattctcc ttcttcagtt ttagcagtcc ctgtcatacc agaaagttta
    11581 ttatataatc taaagaaatt ctggaatgta attgtagcca tagttttaga ttcatctttt
    11641 atttctaccc cttcttttgc ttctatagct tggtgtaagc catctgtata tcttcttcca
    11701 tccattactc ttcctgtaaa ttcatcaact atcattactt cttgattaac aacaacataa
    11761 tcaacatctt tttccataag tttgtgaccc ctaagagcct gatttatatg atgatataat
    11821 tctatatttt ctatatctgt aagattttta atcccaaaaa atgattctgc cttatcaata
    11881 ccactttcct ttagagctac ggtcttatct tttctatcta attcataatc ttcttctttt
    11941 attgttttta taaatgaatt tgctaattca taaagttttg aatcttcatc accctctcca
    12001 gcaattataa gtggagttct agcttcatct ataagaattg agtcaacctc atcaactatg
    12061 gcaaaattca actctctttg tactttatca aactttttag ttaccatatt atcttttaaa
    12121 taatcaaatc caaattcatt atttgtacca taagtaatat cacaattgta ttgaactttt
    12181 ctaacttcag gtataagatc tgcaataatt actcctacag acatacctaa aaactcatac
    12241 actggttgca ttagctcttt atcacgttgt gctagatagt catttactgt aacaacatgc
    12301 acacctttac ctgttaaagc atttagataa acaggtgcaa cttctaccaa agttttacct
    12361 tcaccagttt tcatttctgc tatttttcct tgatgaatta caatgcctcc aattaattga
    12421 accctatatt gacgcattcc taaaactctt tttgaagctt ctcttgccac tgcaaatgct
    12481 tctggtaata aactatctaa agtttctcct ttttttaatc tatctctaaa taggtctgtc
    12541 atttttttta attcgtcatc agacatattt tctatatctt tttctaaaga ttctatttta
    12601 tcaactatca cattaagcct tcttatttcc tgctcatctg ctttatccaa tattgtatcc
    12661 aaaactgaca tatttaattt acctcctttt ttatcataca attactatta taccttaaaa
    12721 ttatagaata gtagacaatt atttttattt atagtataat aatca
//
LOCUS       DAYAOX010000107        12727 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.107, whole genome shotgun sequence.
ACCESSION   DAYAOX010000107 DAYAOX010000000
VERSION     DAYAOX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.107"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(118..609)
                     /locus_tag="ACGI57_002292"
     CDS             complement(118..609)
                     /locus_tag="ACGI57_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717368.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(620..1063)
                     /locus_tag="ACGI57_002293"
     CDS             complement(620..1063)
                     /locus_tag="ACGI57_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717369.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(1053..1892)
                     /locus_tag="ACGI57_002294"
     CDS             complement(1053..1892)
                     /locus_tag="ACGI57_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HFG9717370.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(2111..3496)
                     /locus_tag="ACGI57_002295"
     CDS             complement(2111..3496)
                     /locus_tag="ACGI57_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9717371.1"
                     /translation="MENLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KEYLKKYGFSIDELEGMDTAFAASYGEGRPVIAVLGEYDALDSLSQKVSTIKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(3669..4412)
                     /locus_tag="ACGI57_002296"
     CDS             complement(3669..4412)
                     /locus_tag="ACGI57_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HFG9717372.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSKHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(4432..5694)
                     /locus_tag="ACGI57_002297"
     CDS             complement(4432..5694)
                     /locus_tag="ACGI57_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9717373.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(5878..6876)
                     /locus_tag="ACGI57_002298"
     CDS             complement(5878..6876)
                     /locus_tag="ACGI57_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717374.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(6860..8371)
                     /locus_tag="ACGI57_002299"
     CDS             complement(6860..8371)
                     /locus_tag="ACGI57_002299"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFG9717375.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(8425..9387)
                     /locus_tag="ACGI57_002300"
     CDS             complement(8425..9387)
                     /locus_tag="ACGI57_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFG9717376.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(9435..10325)
                     /gene="rbsK"
                     /locus_tag="ACGI57_002301"
     CDS             complement(9435..10325)
                     /gene="rbsK"
                     /locus_tag="ACGI57_002301"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFG9717377.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(10367..11389)
                     /locus_tag="ACGI57_002302"
     CDS             complement(10367..11389)
                     /locus_tag="ACGI57_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9717378.1"
                     /translation="MNFLSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKI
                     MKEKNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNT
                     DEDAKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETL
                     KGIVKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYN
                     PAYVFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVV
                     GFDDIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRD
                     STKESE"
     gene            complement(11609..12586)
                     /gene="bioB"
                     /locus_tag="ACGI57_002303"
     CDS             complement(11609..12586)
                     /gene="bioB"
                     /locus_tag="ACGI57_002303"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFG9717379.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
BASE COUNT         3918 a         2430 c         1238 g         5141 t
ORIGIN      
        1 tatatgttgc actaagaata tattaaattt agctttatgc tttaatgtat attcatatat
       61 atcatttaca cttatctaaa ctacaatttc attaaaatta aatataaaaa tcaactatta
      121 ttttttaaat agcatctttc ttaatctatt attgtaataa cctataaaag aactgtaaac
      181 atagtcatat actataaagg ctatctcaaa tgaacctata ataaatatgt ttattggaat
      241 ataaactatt gttcttaata aaaaatacaa tataagtatc attatatttg caaatactaa
      301 tttcataaaa tactcaatat atatagacct atctttttct ataagatatt ttacaatacc
      361 atatattcca aaggtaagtg aatatacaat ccattgagac ttacttgcca tcactataaa
      421 tcctaaaact atagaaccta tataaaaagc aattccactc ttaaagcccc attccattat
      481 tacaattgat actaaaagtg atgctaaccc cataataaac aatgtattta caggaataat
      541 atttgtaaga gtcaacaata tcacatttaa tgctaataaa actcctccat aagcaacttt
      601 tatactcata tttatcatcc taacaacaag ttattaaatc tccaccaaaa cattcacaac
      661 atgaatctaa acaccaaagc ttagcacaat tttcacaaca atcgtcacct agacaacaac
      721 agcaatcatc acagcaacag caacaacatc catctaaatc attacgtctt cttctattat
      781 aattgtcagc cctattgcta taatcatttc tatattgtgt ataacttcta agtgcatcac
      841 tatattcact attttcaggc tccataaatt ttgctctttt taagaagtct tcaccttctt
      901 cataatatcc tatgttcatt gcagaaaagc cagttaaata ataccactct gcacatttgt
      961 cagtcaaagt ttttaaaaaa tcatatgctt ttttaaattc actattttct atgaatgctc
     1021 ttgctctcat aaaattttca ttattataag tattactgta cattctttcc acctctactt
     1081 tctaatatat taatatatct taaataaaca ccagaataaa ttatattttc tattattcca
     1141 acatttttat ttagatttag attatcaatt cccctagcta aaaaccctaa tgatgttact
     1201 attatttttt ttactttttc ttttaactca tcatttttat caatatactc tataaatgga
     1261 ttatatcttc ctttcttaaa atcttcatct aaatcttcat aggcatctag tagatatata
     1321 tacttaccta tattaaaacc tatcattctt aagtttctct cattttcatc ttttttatag
     1381 acaaatactt cacccattat atttccaaat atactagata cttcatctat atttatattt
     1441 ttatcttttt ctaacttatt taattcattt agttgttgtt ttatatactc tgctttttca
     1501 ggatactttt catatgctaa ttttaatttg tttttatata tataatatgc caatttatct
     1561 tttatgcgtt tatcatccat taaattatct tccaatttat aatacgttaa aagaatattc
     1621 atacttgctg catattctgt aatatcattt gtaattacct ttttcttttt aaatggattt
     1681 actatacaca cttcctctgt acttatagac tctgggttat atactgctgt taataaaaca
     1741 attaaaaatg ttatatcata attcaaagaa aacctagaga tttctccatg atttcttttt
     1801 aaatatttac atagtccaca atagtaagct ttgtaataat cgtattctct aaatgttaaa
     1861 tccattttgt ttattttaac atatccaaac ataaatactc ctttttgtac tattttttat
     1921 ttagattata tatagtataa cactttttat caagattata taatttacag tattctaaga
     1981 aacttaaaaa attccttcta atcattgtat cttaaattta aaataattag aaggttgggt
     2041 taaaaataat attaaattat atacttatat taactcatac aagttagttg tgcttatatc
     2101 aatttaagtt ttaaataatt ggtatatatt taaaactacc tacagtatcc ttcaattctt
     2161 tttttgcttt atttataacc aattcatcaa gcattacatc atttattgaa catgatatag
     2221 atttagatgc caataacatt gctttaaatc ccatatcaga acctgcactg gcacaagatt
     2281 gccatgtatg ggcagaaata ccaataggcc aaactgaaca agaacactgt gctgtaggaa
     2341 ttatgtaact aacatctcca atatccaatg ataatgatat acagttatta tgagtaaatg
     2401 tatcatccgt aacttcatca tgcagattca tcttagtaat atccttattt ccacttaata
     2461 cactagaaac actacttctt ttatctatag acactgtctc agctaattta tctgcaaaat
     2521 agtagtcatc ttcattatag ttttgtacac ctactaattt catattttgt gatagaatat
     2581 ctgttaatat tgtatttgga atatagtcat atactccact tataatgtca tattccataa
     2641 tagtaccagt cattaatgct gctccttttg ctatatcaac aatcctacca aatacatctc
     2701 ttacatgctc cattttaact cctcttatgt aataccacac ttctgcaaaa tcaggaacta
     2761 tatttggcat ttttccacca ttagtagtta catagtgtac tctacaatta tcaaatatat
     2821 gttctctcaa ataattacaa cctatattca taagttctac tgcatcaagt gcacttcttc
     2881 cgttataagg agcttgtgct gcatgggcac taatcccatt atatctaaat ttcacagagt
     2941 aattagcaag tgttgggatt cttataggag tatttatatc aaatggatgc caactaaaag
     3001 cacaatcaat tccatcaaaa aaaccttttt ttatcatcaa tacttttcca gataaatctt
     3061 cctctgctgg acatccatag tattttatag tccctttcag cttttttagt ttgattaatt
     3121 cctttactgc aattacagct cctattgaac ctgtacccag taagttatgt ccacatccat
     3181 gacctgcacc accatctaca ataggttctt taatagtgga tactttttga gacaaactat
     3241 ctaaagcatc atattctccc aaaactgcta taactggtct accttctcca tagctagccg
     3301 caaatgctgt atccatacct tctaattcat ctatactaaa accatatttt tttaagtatt
     3361 ctttttgtaa atttgaagca taatattctt tatactgaag ttctggattt ttccatatct
     3421 tatctgatat atctcttaat tctccctgta tctcatctat atattccatc aaaaactttt
     3481 tatttaaatt ctccatatta attatataaa cctccctgtt tctaataact tatcgattat
     3541 cagccaaaat gactaatttt aactgattta aaaatatttt ctatcataca accattttat
     3601 acaataaaac ctcttgactt gtaagtttca ttttatcaag taagaggttt tcaagtttta
     3661 taaaattttt agcatgttaa ccaatcaagc atattaaccc ataatttatt atagtatttc
     3721 caatcagtaa attcttttgg accccagtga ggtgcacagt cagaactaaa gatagcagat
     3781 tttccttttc caaactcgcc aacagctaca aatgggtctc ctccaatagt tgctaaaaca
     3841 ggacaattat ctctagctac tgttttgtta tatcctaaaa actttggcca ttctgttggt
     3901 atacctttta atactggatg tttagagtca ataacttctg gaattattcc tgctggtttt
     3961 tcaactctat catctttatc tatcattgtt attggtaata catctttgat agcagtttct
     4021 ccgaatctag cttttgcatc tattcctgta aatgacatat atccacctac catgattaac
     4081 gccccaccat tatttacata ctctttgatt agttcaagtc tatctggatt actattgcaa
     4141 tctataaaag tactattaga taataagaac gtattagaac ctatatcaga taatattata
     4201 cagtcatact catttaactc ttctaattta tacggaaaac tatctgctgc tacatgtgca
     4261 ggcatatgaa ctgtctcata tcctccagat tctattgctt ctttcaacca ctttacagct
     4321 tcttcataat gagttgtaac aaacgtatca aatcctttta tatgagttat attttttacc
     4381 caagattccc ctactattaa tacttttttc atttatatat tcctcctact tttataagtt
     4441 taatattaga cttgcataca taagtatact atctaaatac tccttaattt caacatattc
     4501 atcaattgaa tgacattgtt ctattgaacc tggccctaat ataagtgttg gtatttcagc
     4561 taaatttttc attaatctcg catcattgcc agctgttgaa ccttttatct ctaatttttt
     4621 ccctagagtt tcttctagta ttttatgtgc attcactaca aaatcagaat ccttgtccat
     4681 ctcaaaacct aaccctgatt gatatatgtt tatatttggt ttattatctt taagccataa
     4741 atctccatta gcccttgtca ttatgacatt tgttacatca tttacaactt gttcataact
     4801 cattatattt ggcaagaagt gtacacataa attaaataca cacttatctg gcacagtaga
     4861 acctgctgtt ccaccattta ttacacctaa atttattgta ggtgaaggta atagtggatg
     4921 tttatatatc ataagccagt tatgttccaa ctcttttata tcttgaagca acaacatagc
     4981 cttctcaatt gcatttacac cctcccactt actaccacag tgaagagaca cacctttaac
     5041 ctcaacttca aagaatacaa atcccatatg tgcaactatt aagttttgtt ctgatggttc
     5101 acaaattatg caacaatctc catcaatacc attcataact gcatttatgg taccatttcc
     5161 gccaccttct tcatctacta ctgacattat ttttacatta ccaggtacat ttagaccact
     5221 atcttttata agctttacag ctaatattga tgctataagt ccagatttca tatcagcagt
     5281 tccaagtcca taaagttttc cattatcttc tgtagcacga tatggattat atttccactt
     5341 acttatatca cctggtggca tagtgtctac atgaccatta aatacaattg tcttaccagg
     5401 taaatcatca ctatacttac aaattaaatt atatctatct tgataattgt gacctagatt
     5461 tccttcttta tatatatttt ttgctttttt aattaattct tcgctcattt cttgtctatc
     5521 tacactaaaa cctatgtaat ttgcaagttt ttcaatatat tcctgtcctt ctttttcaaa
     5581 tccacctagt attccatgac caacatcttc agtctttata cttacaagtt cttttaaata
     5641 atctatgtat tcttgttttt tactatttaa tgtatctaag agaatttttt tcatatttcc
     5701 tcctaagatt tatttttaca tttaaaattt caagtctaat ctcatctatt taagccatag
     5761 taaacttttt cacttagcat attcccattc aatgtataaa gatgtatgca aattaaagta
     5821 taaaaatata tacaaaaact taataatttt tatagtttat aagctaaaat ttaattacta
     5881 tttagattta gctgattttt ttaattttat attatttatc catatagcaa ataacattac
     5941 caaacctttt ataatcattt gattatatga agatacactt aataagttta atccatttcc
     6001 taatattcct attattccga cacctataca tgtacctact atagaaccag aaccacccat
     6061 catattagta ccaccaagca caacggctgc tatagcatcc aattctgctc catctccaac
     6121 agttggtgaa cctgatgcca aacgtgatga tagtattatc ccagctaatg ctgttagtag
     6181 tccagctagt gcgtatactt ttactataac tttatttact tttactccac ataatctagc
     6241 ggcctcttca tttccaccta ctgcatgtac atgacgtcca aaattagttc tttttaatag
     6301 aaaatgtcct gctattacaa ctataatcat aattataact ggtattggta ttggtccaat
     6361 atatcctcta ccgagcatct taaatgcatc tgtatttact gatataggtc ttcctcctgt
     6421 aaatacaaag cttgccccac ttattatcgt taacatggct aaagttacaa tgaatggagg
     6481 tagtttttgt gttgaaataa ttgcaccttt tatgactcct aaaattgtcc ctattgctaa
     6541 tgcaagtatc atacctaccc atataggtgc attataattc actactgttg tagcccaaaa
     6601 tgtacctact aaagcaatta ctgctcctac acttaaatct attcctcctg taattattac
     6661 aaatgtcata cctactgcaa gtattgcatt tattgatatt tgtcttccaa cattaattaa
     6721 attatctaaa gttaaaaagt tagatgaagc tacactcatt attataaaaa ttattacaag
     6781 tcctacagct gcactccctt ggtctctaaa tacacttgtt aattttgtca ataatggaga
     6841 agatttttct tctttatgct tagttattga actcattaat tttacctccg aaagccaatt
     6901 ttaatacatt attttcaacc atttcttcac cttctaaaaa tccacttata tgaccttctc
     6961 tcataaccat tattctgtca ctcattgata ttaattctgg tatttcagaa gatatcatta
     7021 ttatacttac accactctcc actaaatctc ccattagatt gtatatctct gccttagcat
     7081 ttacatctat acctctggtt ggttcatcca taatcaatat atctggttgt aataaaagcc
     7141 atcttgctat tatagctttt tgttggtttc caccacttaa actttgtata acttgattca
     7201 tatgtggtgt ttttatcttt aaagcttcta tgtatttttc taaaatctct ttttcctttt
     7261 ttctatctat aattgattta aacttagata tcttttttaa cccagccata gttgtatttt
     7321 ctttgacact catttgtaaa aacaatccag taccttttct atcttctgtt attaatccta
     7381 gtccattttc tattgcatct gatggagttt taaactttac ttcttcattg tttaaatata
     7441 tttttccaga atcatatgtt ctaagtccaa atatagcttc tactatttca cttctcttac
     7501 ttccaactag tccacctatt cccaatattt caccttttct tatattaaag ctaatatcat
     7561 taaaaacatt gttcttagtt agattttcca ctcgcataat ttcttctttt atttctactt
     7621 ttctttttgg gaaaaattgg tctagctttc tatctatcat taaattaact aattcttcct
     7681 cagacgatac atctttggta tttaaagtcg aaatcgtttt cccatctcgc attatagtta
     7741 ttctgtcaca aatctcaaag agttcttcca tcctatggga tatatatatt atactaactc
     7801 cactctcttt taaatcattt ataagtctaa aaagtatttc agtctctttt ttacttattg
     7861 aagaagtagg ttcatccatt attaatactt tagtttctga tgataaaact cttgctattt
     7921 ctaccatttg tttttgagct atactaagct ctttgactaa actatttgga tttatatcta
     7981 ttcccatatt atcgaaaatc tttttagctt cttcattcat ttttttgtca ttggttaaga
     8041 ctttgccatt tatttctttt cccataaaaa tattttgtgc tactgttaat tcgtcaaaaa
     8101 ctgttagttc ttgatatatg gcatttatac ctatcttttt agagatatct ggggatatat
     8161 tctctatttc tttgccttca aaaataaatt ttccagaagt tttcgaatgt gccccagtta
     8221 aaatctttat cattgttgat ttaccagcac cattttcacc cagtagtgca tgaacttcgc
     8281 catgaaataa ctcaaaatta acattatcta atgctctgac accaggaaat tctttagcaa
     8341 tattacttaa cttcagaatt atattactca taatgacact cctttttaaa taggatttct
     8401 ttcaaataat ctcattttat tttactaatt cttaaagtct tttatatttt ccttatcata
     8461 aagtaaagct ggtactggta tattcttttc taccttttcc ccttttatag cttttactac
     8521 tgcttctaca gctaaacgtc ccatctcttt aggttgttgt tgaatcatag caagaatagt
     8581 tccatctgtt attccacttg atgcttcttc agttaagtca aatcctacaa tcttaacatc
     8641 cttatttttt gcagattcaa gtgcagcttt agctcctagt acactatttt cagctgttgc
     8701 atatattata tctacatctg gattagattg aattaagttt tctactgtat taagagattc
     8761 ttctctgtca tatcctggtt gtgaatttaa tatcttaaca tttgctgaac cttttaatgc
     8821 atctttaaat ccatctattc tctgcatttg tatttgagat tttaaatctg tcactattgc
     8881 tatttctgac tttccaccta acttttcatc tatgtatttt ttagtgtatt cccctaattt
     8941 tttaccagca tcataactaa ctgttcctac ataacttgta atattttcag tatttaatac
     9001 agcatctact gtaactactg gcattttttc aaatgctcct tctactgctg gtactatagc
     9061 cgaattatca gttccaagta ctatcattcc atctacattc tgttgtataa aatcttctat
     9121 tgctgatgtt tgcttagcta catcttggtc tgggtcattt actataactt tcacacctaa
     9181 ttctttagct ttttctttta ttccctcatc aatatctata aaaaattgat gttgaagagt
     9241 tattgtagat actccaatag ttaaatcttt cttgtctgaa ccacctttat tatctgattt
     9301 acttggtcct tccattgaac accctgttaa cattcctatt gctagtattc cagcaattcc
     9361 taaagataaa atttttctca ctctcataat taacctccat ttttaatagt tttaaatttg
     9421 aacaaattag atttttattt attatttaaa tacttttcaa catcttcttt atagggcata
     9481 ccagattgtg cacctagttt agttacagaa agacctgata ctactactgc atattctact
     9541 gctgtatata aatttttccc ttctgttata gccactgcca atgctccatt aaatgtatct
     9601 cctgctcctg tggtatcttg aacatcaaca cttatacaag atacatgctt gatttcctca
     9661 ccatcataaa atctagctcc attttttcct tctgttatta caagtttatt tggatatttt
     9721 tttaatactt cttctatccc ttcatttgtg tcaaaaacta tcttatattc atgttcattt
     9781 ggtgttaagt aagttacttt ttctattata tcttcattta gttttactgc tggtgctgga
     9841 tttaataaaa ccctaatcct attttcaaaa cagaaattca ctacataatt tattgttttt
     9901 aatggaatct ctaattgtag caatactata tctgcattct ttatctcttc ttcattcttt
     9961 ttaattaatt ctatatctac tagctcatta gtccctggta caacaacaat actattatca
    10021 ttttcacata atgttataac agcaacacca gaacttttgt gattatttct ggatactaag
    10081 ctataatcaa ctttattcgt tctaaaattt cttattaact cctctcctaa gccatcttcc
    10141 cctatgcatg atatcatttt tacatttgct cctagtttag aagctgagat agcttgatta
    10201 gccccttttc ctccaggtga tgtaaaaaaa ctattaccta atactgtttc tcctttttct
    10261 ggtcttttat ctacagaaca taccatgtcc atgtttacac ttcctatgac aactatattt
    10321 cccattacat tactccttta catatctatt tatgtctctc gtgctgttat tcactttctt
    10381 ttgttgaatc tcttatgacc aattctggtt tgaaaatcaa ttcatcaaac gattcttttt
    10441 tatctcctct taaacaattt atcaatattt cactggcttt atatcctata tcataagacg
    10501 gctgttttac tgtggtaaga gaagggctta ttagacttga tgtttgaata tcatcaaatc
    10561 ccacgacact tatatcattt ggtatactta atcctctttc tttaagacct cttatgacac
    10621 ctattgcaat caaatcattt gcacaaaata ttgcatcaaa atctatcttt tctaaatttt
    10681 ttataaattc ttgtccccat tcaatcttat attttccttc aaatacataa gcaggattgt
    10741 agcttaaatt acactcgatt agagcctttt tatatccttc taatctatct actgctattt
    10801 cattttgaag tggaccactt aggtatatta ttttcttatt tccaatatct aataaatgtt
    10861 ttgtagctat ataggcccct tcaaagttat ttattttaac aattcctttt aaggtttctt
    10921 tgttcatact tatcttctta tcaatcaaga cagttggtat ttttatatct ttataatttg
    10981 cttctttcca gtttgtattt gaagatgctg caaatataat tccatctatc atcctctctg
    11041 atagtgttct tattccttca tattcttttt tagcatcttc atctgtatta cataaaatta
    11101 tattgtaacc ttcttcatta gctttgtctt ctacacctct tgatacatct gtaaagaatg
    11161 gatttcgtat atctggtata agaagcccta tagtattagt ttttttagtt actaaacttt
    11221 gtgctaattt atttggtaca taattttttt ccttcattat tttcaaaatt ttttctcttg
    11281 ttgcctgact tatattttca tctttattat taatgacttt tgacacagtt gttttagaaa
    11341 caccagccaa ttcgccaatt tcttttatag ttatactcaa gaagttcacc cccaaaattt
    11401 aggaaaccgt tttacttaac cggttaccta gatactatca catctttttt ccatttgcaa
    11461 ccattttata taaattttga tatttttccc ttttttcaaa atattgcaac ttataattca
    11521 attttaatat ttaaattaat ctgcaaaaaa aaattaatat acttttataa ttatctaaaa
    11581 gtatattaat taatttaagc agtatttatt aaagttcaaa tccttgctct ataatcattt
    11641 ttttatcatt ttcaatccca cttccaacag ttgtcaaata atcaccaaca attgcagaat
    11701 ttactccccc attaaatcca actttgtcat atccctttaa tgatagcctt ccaccagcat
    11761 accttatgta cacattagga attataaacc taaatactgc tgttgttttt agtatttcca
    11821 ttggttctaa tacatcataa ttttccattg gtgtattctt aataggattt aaaatgttta
    11881 ttggaaatga ttttacattt aattctcgta tttcaaaagc catttttagt ctctgttctt
    11941 cactctcatt cattccaatt ataccaccta cacatacatc tagcccagct tttttagcat
    12001 cttttatagt ttttatccta tctttatatg tgtgtgttgt acatatatta tgaaaattat
    12061 ctctacacgt ctcaacattg tggtgatatg tagtcacacc tgctgacttc aacattttag
    12121 cttgctcata atctataata cctaaagatg cacataattt taaattagta ttttccctca
    12181 atccttcata gatatttaaa atattattaa actcctttcc tgacatacct ttaccacttg
    12241 taaccaatga gaatctatgc actcctttac tctccatctc tttagctcta tttaagattt
    12301 cattgtaatc taataatgaa tattcttcta caccagtttt ataatgagat gattgagcac
    12361 aaaatttaca gtcttcacta catttgccag atttagcatt cattatcgta cataaatctg
    12421 cttttcttcc cataaaatat tctcttattt catttgcaga ttttaataga atatcaaaat
    12481 cgtttttatt atttgtatca aggcttatta aattaagagc ttcttcataa cttatttctt
    12541 tttctctcaa tactttattt tttaatttta ttatgtattt ttccatatat cctcctattt
    12601 gtcaacttaa tatttttatg tagtttactt attatatata gtattgcata tataacattt
    12661 attggtcaac tttattttag acacaagttt actatacaca aaaaaatact gcttcaaaat
    12721 aatatta
//
LOCUS       DAYAOX010000108        12697 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.108, whole genome shotgun sequence.
ACCESSION   DAYAOX010000108 DAYAOX010000000
VERSION     DAYAOX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.108"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            144..881
                     /gene="cas6"
                     /locus_tag="ACGI57_002304"
     CDS             144..881
                     /gene="cas6"
                     /locus_tag="ACGI57_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HFG9717380.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPINFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            886..2361
                     /locus_tag="ACGI57_002305"
     CDS             886..2361
                     /locus_tag="ACGI57_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717381.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENRLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYSFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKRMIDNGNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2381..3394
                     /locus_tag="ACGI57_002306"
     CDS             2381..3394
                     /locus_tag="ACGI57_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HFG9717382.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3410..4207
                     /gene="cas5"
                     /locus_tag="ACGI57_002307"
     CDS             3410..4207
                     /gene="cas5"
                     /locus_tag="ACGI57_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HFG9717383.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYIKMGKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVKLKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4234..6642
                     /locus_tag="ACGI57_002308"
     CDS             4234..6642
                     /locus_tag="ACGI57_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFG9717384.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKSTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGVNKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            7083..8030
                     /gene="prmA"
                     /locus_tag="ACGI57_002309"
     CDS             7083..8030
                     /gene="prmA"
                     /locus_tag="ACGI57_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFG9717385.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            8049..8807
                     /locus_tag="ACGI57_002310"
     CDS             8049..8807
                     /locus_tag="ACGI57_002310"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFG9717386.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEIILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNSVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8809..10107
                     /gene="mtaB"
                     /locus_tag="ACGI57_002311"
     CDS             8809..10107
                     /gene="mtaB"
                     /locus_tag="ACGI57_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061712 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HFG9717387.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10150..10500
                     /locus_tag="ACGI57_002312"
     CDS             10150..10500
                     /locus_tag="ACGI57_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFG9717388.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10663..10842
                     /gene="rpsU"
                     /locus_tag="ACGI57_002313"
     CDS             10663..10842
                     /gene="rpsU"
                     /locus_tag="ACGI57_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFG9717389.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10872..11315
                     /locus_tag="ACGI57_002314"
     CDS             10872..11315
                     /locus_tag="ACGI57_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFG9717390.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11426..11923
                     /locus_tag="ACGI57_002315"
     CDS             11426..11923
                     /locus_tag="ACGI57_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFG9717391.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12116..12679
                     /gene="raiA"
                     /locus_tag="ACGI57_002316"
     CDS             12116..12679
                     /gene="raiA"
                     /locus_tag="ACGI57_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFG9717392.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
BASE COUNT         5392 a         1121 c         2311 g         3873 t
ORIGIN      
        1 agaataaaac taacagtaga atatatttaa aaaattaaaa aacaaaatta aatttattat
       61 caaaaaatca ttttatgata tgttttattt ttatattaca aatagagaat aatgaattat
      121 actagaaagt aggaggtgaa aatatgagca gaatttcaat ttttatggaa tcatgtaatg
      181 gcaaatttcc aaatgaaaat tcaatgttgt cagtttcctt tataaaaaat attttaaatg
      241 cagaaaacaa agattttgct aaaaacatat ttaactatgg ggaagaaaga aaaaacaata
      301 agcaaataaa ggatataaat acagccattt acataccaaa tcttataaga agcaaaaatg
      361 agttacttgt tgatggagat ataatattca atataagttt ttataattat tctatgttca
      421 gtaaattata caatggaatt ttaaaagtaa aggagctaga atataaaggt tatagattta
      481 aaataaaaaa tataaaaata cataaagaac acgagataaa agaaaatggt gttatattta
      541 aaactatgtc tcctataata gtaaagaata aagaaggtaa atatttagat gtagaagatt
      601 caaattatat agaatgttta aactatatag ctaatctaac tctggagagt ataagaggta
      661 gtgggcttag aaagccattg gaatttatac ctattaactt caaaaaaaga gtgttaaaag
      721 aaaagattcg tggatttaaa gtgagagaat attactatat aaatgcttat gcaggaacat
      781 tttttttaaa aggagatatg gaagatttaa atgcactgta taaaatggga attggatata
      841 gaaggacaga aaatgcagga atggttgata tattaaagta gcgaggtgag agaaataaaa
      901 gctaaatttt atagatcagg tgatattgta agagatattg gcattgttta cttttatgaa
      961 ttactaatgg atttaaaaaa cgaactagaa aaaaatgatt ataagctaaa atttacttgt
     1021 gagttaaata gaaattattt atctttagaa tctgcagaat atatagaccc taagtatata
     1081 tctgattata tattagaaaa tcgacttttt aaggtattta aaaatgggaa aaaggaaacc
     1141 ttagaaaaaa tatttagtaa tatagataat ttaacgtatg taaattttat tgaagaatta
     1201 gataaatctt ctgcaactgc taaacaaaag gaagatatta aaaaagattt taaaaacagg
     1261 aggtttccat atgttagaaa tagtggtaag tatggattaa acacgagttt agaaaatatg
     1321 gaaacaaatg taaaaagaat agtagatact gtaattaaat caaatataga gatagatgaa
     1381 atagataaac ttgatttgac acaatatgaa gaaaaagata gtgtatgtaa tgtttgcaat
     1441 ataaataaaa caacaaaact agatattgat ttaagagaac gaaaggactc taagtataac
     1501 tttttattta gaggtgcaga aaaatcaggc tttaaaagaa gtggacaagt tgaaagtaat
     1561 atttgttttg agtgtgaatt tttcaactta atgtgtcttt tatatataaa tttaaaaaga
     1621 ccaatggttt ttgcatatac gaatgattta agagaattag catttttaaa tcataagata
     1681 atgcttaaaa gaaaaatgta ttcagataaa tcattttata aaaaactttt gcatgaaaaa
     1741 ataagttcaa ttagactata tagctttgat attgatacga ataaaggtat aattctaaag
     1801 tttgattcta ttatagagta taaagaatta ttaaaaaaaa ttgaacttat agatattata
     1861 gataattata atttttcaag agagccaggt aatactagaa atttgggtaa aagaatgatt
     1921 gataatggga atttatcaaa tttaaaagaa ttattacttg ataatataag tgttttaaag
     1981 caatcaactg gaacttctcg agaaatggat tttgtatcta gctcatataa tatcggttta
     2041 tatataaaat tgtgttggat tgtagatgga ggggaggagt ataaaatgaa aaactatatg
     2101 gaaagtaatt taatatattc aagagtaggt aaagacttat ttaataaaat gacagatgaa
     2161 tccagaagaa aaaatttttc tatgaaagtg attcaactac ttaagtctaa tgatagaact
     2221 cagctatttc aaactataat gcatgtgtta gtttctaatg gaatcttaat aggagaaggg
     2281 tttgtagaag gtctaatgca gagttctgaa ttagaattaa atacgaatgt aggtctattt
     2341 attcaagaaa tcatgaaata aaaaaggggg aaaatttaaa atgaatgaaa aaagagtaaa
     2401 aggagtaaca ttaactatac tgacagaaag cccagttcca ctttcatatg accaagggta
     2461 tggaaattat acaccaataa aaaaagaaca atatagagaa aaaatacatg caaaaactag
     2521 tatagccaca ataacatatg atttaagaag aatgctccac caagaatatg gttggaattt
     2581 aagtaatatt gtttttggaa aaaaaggaaa tatatatcca tctattaaga aaaatgtagg
     2641 atgtgtagat gaaaatggat tagagactga tgtatttggt tatttaatac cacttaaaga
     2701 taaaggttct ataagtaaag cttctccact tagaataata ccttttaggt cattaaatcc
     2761 atataaaggt tcaacacagc taataacaaa tagaggcttt ttaagtagtg aatttggtag
     2821 aaaatattat gatgagaagg aagaaaatga agtacctaga gatgaaaact ttccaacatc
     2881 acaagcatta gcaatagaag agactttatc agattactac gtttatacaa taacattgga
     2941 gcttgataga ataggagtag tagaagtaga agatggaaag ttacttttac cagaagaaag
     3001 aaaatttatg agtaaggagt taagagaaaa agcagtaaaa gatatattag atgcaattac
     3061 tgtgtttaca agaaacataa aacattcatc agtactatta aaaccacttg cagtaatagg
     3121 tggagctttt gataaagttg taccattttt ttgggatgat gtagattata atgctgatag
     3181 tggtgagata aatttagagg gagtcataga aactatagaa agttatagcc ttaaagaaag
     3241 taacacaata cttgctatca atgatagatt aaaaatttct aataaaaagg aattaaataa
     3301 gtttaattta agtaaatacc ctgtaaaaga gattaaaaat ctagcagata gattggaaat
     3361 tggagaagat aatatgtggt atttaaaaga gtaggaggtt ttttgaatta tgaaagtatt
     3421 acaatgtaaa cttaagcaac caactgctca ctatagagac ccaaaagtat ttcaaaatga
     3481 atatataagt actttaaatt taccatctaa gaccacaata atgggaatga taacgtattt
     3541 atgtgataga agattaaact ctgatataga tataggaata attggaacac atcatcatag
     3601 agaactagaa ttttcaagag gagaaaacat tgatttttgg aatgaatata taaaaatggg
     3661 aaaaggtaaa gataaagaaa agtttttgct taaaggaaac tactatgatt actataaaga
     3721 gcataaagct caaaatagca tattaaacta tgaagttctt aaagaagtag aactgactat
     3781 atttatttct tgcaaggatg atgaagaact tgaatttatt agaaaaaagc ttgaaagtcc
     3841 atgtaaatat gcaaatttag gaagaaaaga agattttgta ataccaagtg aaaaagggtg
     3901 ttttgtgaaa gaagttaagt taaaagaggt tatacctatg aatactcgtg atgctataaa
     3961 agaaaatata aaattaaaga atacatatgt aagagttgac ttaagagata aagagaatgt
     4021 cgagactata ataaatcaag gtgtattgat tgcattgcct cataaatata aagatttaga
     4081 agcaaataga gatgatagag tttatgagtt ttgccattat atatatgttg ataatgatgg
     4141 tatatatcct aaaaatatta aggtaaatgt atgtattgat acaaaagaag tttttacatg
     4201 gctttaaatt attttgaaag ataggtgttt atcatgaaaa atatcatgca aaaagaaata
     4261 ttagctaagt catacaattt aagaactaat caacaagaac aaacattagt tgaacatatt
     4321 aaggatttat ttgaagtatt agaatctata ttagaattaa atctatattc agataaagat
     4381 gtagagatac taaaaatatg ttgtgctttg cacgatttag gaaaaattaa ttcaattatg
     4441 caacaaaaaa tggagattaa taataaaata tcgtatagtt gctcagaaga agagaggaaa
     4501 aagttagagt ctgacaaaaa atctttaaaa aagatagcaa gacataatat attttctgga
     4561 gcatttttaa aagatatatt agaaaaaatg aacttgtcag aagaagataa gttatatata
     4621 tataaaagta taatgttaca tcatggaaac tatgaagatt atatgaggtt aagtattagt
     4681 aaagttcaag aagaaattta tgagtatatt gaaaaaggca ttttagaaaa agaagagttt
     4741 aatctgaaag atatagaaag ttatataaat agcattttaa atgtagactt taaatttggt
     4801 gaagatgtat tggattatga ttacatagat aaattaagtg aaagtatgtt tatagacagt
     4861 gattataaca atggtcaaat agatgattca gttttaaatt atagaaagtt taagtatatt
     4921 ttatataaag ggatgctgaa cttaatagac catagtgcat caagtagaca gaaaggtata
     4981 aaattttata acgattttac tgatgaagaa attgataata tgattttaaa tgagatatat
     5041 aagagtcagg gtaatttgga aaaaaataat atagagttca atactataca aaaaaggttg
     5101 agagaacttt ctggaagaaa tgttctgact gttgcattta caggttcagg aaaaacagct
     5161 gcagattata gaaagacttt taaaagaaaa ttctttttag tgccaaataa aatatcagca
     5221 gaaagttttt atagaaaaaa tatttttcaa aataaaaatg atttagatac aagaagtagt
     5281 aatgattaca ttggattact tcatggagat ataaacttat attcagagaa tgaagataat
     5341 ggagaacatg attttgtatt gacattaagg gatatagatt tgagtataaa cttttgcaaa
     5401 ccttgtgtaa ttgcaacagt tgatcagtta ttacttagta tgtttaaatt tccgggatat
     5461 gaaaaaatat tcgcagccgt aaaaaatgca tctataactg ttgatgaagt acatttattg
     5521 gaaccaaaga tgtttttgat tttgatatat tttatgcagt ttgcatgcaa gtacttagat
     5581 gttgattttc acttaatgac agctacactt ccaaaatcat ataaagagca aatgataaac
     5641 aagggaatta tttttcaaga agaaagtaat gaaaatgtta ctgataaagg agaaatagtt
     5701 tttattgaga gcaacaagga agaagagatt tgtgaaggta aagatgtaaa ggtttctttt
     5761 ataaaggaaa aagaaatcaa gtcaatagtt gaagatgctt tagaaaataa acaaaaaatt
     5821 cttataataa agaatactat tgattcagtc aatagaacat atgagttttt aaaagaaaat
     5881 ttatctgata agtatggtgg tgtagatata gatgttttac atagtagatt taagttcaaa
     5941 gataaaaaag agaaatatag taaaatatta aatggagagg gtgatatttg gatatcaact
     6001 caatcagtag aaatatcctt ggatttggat ttcaatataa taataagtga tttagcaact
     6061 atggatagct taatacaaag aatgggtaga tgcaatagaa ataataagta tgaatatgga
     6121 aacttttata ttctacctag tgaagataaa atttatgaca acaaacttaa gagcacaact
     6181 aaaaacatct taaaggacat attaaaaact aactctattt ttacaatggg aattagaaaa
     6241 actattttgg atgattacta tgataatagt gtagtcaaaa aatattttga agagaatttt
     6301 ataagttgtg ataaagaaat taagaatatt tatggtgtaa ataaagaaat ttttgatgga
     6361 ttggatttaa tttttaattt tgaaccttac aaaaatattg ttgatagtaa gaaggaagca
     6421 gctaaaattt ttagagatgt tgatgttagt tacaaaatta ttctggaaga agatttttat
     6481 aaggaagata gagagttaca acaagattcg attcaagtat cagggtttat atttaataga
     6541 ttatattatt atgggttaat aagcaaagta gaaggataca tggtactaaa atctagtagt
     6601 aagtttgagt ataatagtac tgtaggctta attttgaagt gagcatatgt ttaaatataa
     6661 tataccttta gacattgaaa atacaaaaag tactactgta agctcactgt aaaaactatt
     6721 ttttaaaagg aaaaataagt tgtgagatag agtgtttaca agtgtatata gataataaat
     6781 ttgaggttta agattaacta agtgggtttc aggtacatcg cgaaaataat tataagttgg
     6841 agattttgta tgtttaagat taactaagtg gatttcaaat attgatatag tagatataat
     6901 agaagtttta ttattaggtt taaaattaac taatcggatt tttaaatatt ctgactatta
     6961 tataaggata ttattagatt gaatttaaaa ccaataatat aaaacaacaa aatattatat
     7021 ttatgatata ataaaagtta tggaaaaaca ggaatattac tatagagaag agaggtaaac
     7081 ttatgaataa ttggatagaa gtaactataa aaacaacaac agaagcagtt gaacctataa
     7141 cgaatatact atatgaacaa ggagctggag gagctgtaat agaagaccca aaagactttt
     7201 tattccaaaa gaaaaatgaa ctagactggg attatgttga agaagaagta tttaaaaaga
     7261 atgaagaaga tgatgttctt ataaaaactt atgtttctga agaaaaaaat gtgatggaat
     7321 ttgtcgaaat aataaagcaa aaagttttag gattaaaaga ttttggtata gatataggtg
     7381 aaggaagtgt atcattggac caagtaaatg aagctgattg ggcaaatgca tggaaagcat
     7441 actacaaacc aacaaaagta ggacaaaggg tagtagtaaa gcctacatgg gaagattatg
     7501 ctatgcaaga gggggattta atcatagagc ttgaccctgg aatggcattt ggtacaggta
     7561 cacatgagac tactagtatg tgcattagag aattagaaaa gtatgtgaat aaagattcta
     7621 aagtatttga tattggatgt ggtagtggaa tccttgcaat agcagcagct aaattaggag
     7681 caaaagaagt tgtagctgta gatttagatg aagtggctgt aaaagttgca aaagaaaatg
     7741 ttcttgaaaa taaagttgaa aaaagtgtat ctgtaatgca tggtaattta actgatgtaa
     7801 taaaagataa agcggatgta atagttgcaa atataatagc tgatataata aagatacttg
     7861 caaaagatgt tcaaaacttt atgaaagaag atgctatatt catatcttca ggtataattt
     7921 tggataaagt agaagaagtt aaagaaagct taattgaaaa tggatttgaa atagttgaag
     7981 ttcaaaaatt aggtgaatgg tcagcaatag tttctaagct taaaaaataa gggatatggt
     8041 gacgtataat ggatagattt tttgttgaaa aaaataatat aaatctacaa gataaaactt
     8101 gcactataga aggtgaagat gtaaaacata tttcaaaagt tttaagatgc aaattaggtg
     8161 agaaacttga aatatgtgac aagaataata atgagtatat ttgtgagata atgaatatag
     8221 ataagagtat agttaattta gaaattttag aaaaagttga tataaataga gaatcagaac
     8281 ttaaagtaag gctataccag ggacttccaa aagctccaaa aatggaaata atacttcaaa
     8341 aattaactga agtgggtgta gaagaaataa tattagttca aactaagaga agtgttgtta
     8401 aagttgatga caaaaaagaa gataaaaaat ttgaacgttg ggaaagaata atttatgaag
     8461 cagcaaagca aagcaaaaga ggtaagatac caaaattaag aggtgtttta agttttaaag
     8521 aagctttaga agatatgaaa aaaaatagtg taaatatttg cccttatgaa aatgaaagaa
     8581 cagtttcaat aaaacatgca ctaaaaaaat gtgattctaa tatagatagt gttggaattt
     8641 ttataggtcc agaaggtgga ttttctgaag aagaaataga acaaatacaa aaaaataatt
     8701 gtaatgttgt atcattgggg cctagaatat taagaactga gacagcttct gttgtagcat
     8761 caacaatagc tttgtatgaa ctaagtgatt taggaggaga aaaataaatt gaaaaaagta
     8821 gctttttata cacttgggtg taaagttaat caatatgaaa cagaagctat gctagaacta
     8881 tttgagaaag atggttatga acaagtcaat agtgaagagt atgctgatgt ttatgttata
     8941 aatacatgta cagttactca tatgagtgat agaaaatctc gtcaatatat aagacgagtt
     9001 aaaaagaaga atccagatgc aataatagcc gttgtaggat gttattctca ggtatctccg
     9061 gaagaaattt tggatataga agaagtaaat ttagtcatgg gtacaaatga tagaagaaag
     9121 attgttgaag aaattaaaaa gataaattca agtaagaaag ttagtacagt tgatgacata
     9181 atgaaagtta aagcttttga agaaatagaa atcagccaaa ctaatggaaa gactagagca
     9241 tttatgaaaa tacaagatgg atgtgacaga ttttgtactt attgtatcat tccttatgcc
     9301 cgaggacgcg ttagaagtag agatattgat agtatagtcg atgaagttaa aaaattggct
     9361 aataatggct ataaagaggt tgtattaaca ggaatacatg ttgcctctta tggaaaagat
     9421 ttaaaggata gggatattaa acttcttgat gtaataaaac aaataaacca gatagaaaaa
     9481 atagaaagaa ttaggcttag ttctgttgaa ccaattttat ttacagatga atttgtaaat
     9541 gaagttttaa agatggataa agtttgtcct cattaccatt tatcacttca aagtggatgt
     9601 gatgaaactc taaaaagaat gaatagacga tacacaactt tagagtacaa aactattgta
     9661 gatagattga gaagtaaaat gccagatgtg gcaataacta cagatgtaat agtaggtttt
     9721 cctggagaga caaatgaaga gtttaaaaaa acgtacgagt ttttaaaaga aatagagctt
     9781 tcacaaatgc atatttttaa atattcacca agaaaaggaa ctccagctgc aactatggaa
     9841 aatcaagttg accctcaaat gaaacacttt agaagtgaac aacttttaaa tttaagtaaa
     9901 gtaaatttta ataaatttgc aactaaattt atcggccgtg agttggatgt tttatttgaa
     9961 caaaatatag aaggcaataa atatgaggga ttaacttcaa attatataag agttgttgtt
    10021 gaaagtgaca aaaatataca aggtcagata ttaaaagtga aaataaatga tgtaaaagat
    10081 gaatatgtag aaggaatttt gctataacat gtagaaatta taatatttat aagataggag
    10141 gtggaaagaa tggattgtat tttttgtaaa atagcgaacg gagaaatacc ttcaactaaa
    10201 gtttatgaag atgatagagt cttagctttt aatgacttga acccagttgc tccatatcat
    10261 atattagttg tacctaaaaa acattatgat agtttaattg atattcctga taaagagatg
    10321 gacattgtgt cacatattca tgttgttatt aataaaatag ctaaagagaa aggttttgac
    10381 caaactggat tcagagtcat aaataattgt ggaagtgatg gagggcaaga agttaaacat
    10441 ttgcactatc atattttagc tggtaaaaag cttcctaatt acgaagcggg acaaaactaa
    10501 actaaattat tgaaaaattg agttccttct tgataaaaat acttgataaa agtaaaaatt
    10561 tagtttataa taaacaaagt gttaggttgt aatgcatatt atgcaaaaag aacatagtta
    10621 cagtcccagc ataattgcgc tattggaggg agggaaaaag aaatgtcaga agtaagagtt
    10681 agagagaatg aaacattaga cagcgcttta agaagattca agcgtcaatg tgcaatgtct
    10741 ggcatcatgt cagaagtgag aaaaagagag cactatgata agccaagcgt taagcgtaag
    10801 aaaaaagctg aagcagcaag aagaaaaaac gctaagaaat agtagtaaat aagaataaag
    10861 aggtgaaggg gatgtccctt aagcaaaagt tacaagagga tttgaaatct tcgatgaaaa
    10921 acaaagatac agtgagaaaa tctgtagtaa ctttaataag agcttctata aagcagtatg
    10981 aggttgataa tagggtcgag cttgatgaag atggaattat cgatgtaata gcaaaacaat
    11041 taaagcaacg tagagatgca ttagttgaat ttgaaaaagc tggaagagaa gacttaataa
    11101 aagaaacaga aggtgaaatt gaagttttaa aagagtacct acctcaacaa ttaagcgaag
    11161 aagagttaga agaaattgta aaatctacta tatccgaagt aggggcaact tctatgaagg
    11221 atatgggaaa aataatgtca gttatccaac caaaagttaa aggaagagct gacggaaaac
    11281 ttataaataa gttggtaaaa caaaacttac aatagattga tatagcctag agagaaatct
    11341 ctaggctttt tatagttttt gggaataacg agtcatattt gagcatatct ataatcaaag
    11401 aaaattttga aagaagggga aaaatttgat tatatcagta aaagagatta ggaatttttt
    11461 gataaacata tttataccac taataatagg ttacttaagt agcatcttaa caatgattat
    11521 cagtggaaca gatatttcaa cctattattt acaactcaca aagccaagct ttgcaccacc
    11581 agcttttatc tttcctatag tttggactat attgtacata ttgatgggta tatcttcata
    11641 cctaatatta agaaagggtt acaacttacc taaagtaaga gatgcaattt tttattatgg
    11701 attacaactt gtattaaact ttatttggag tatactattt tttggatttg gacttagatt
    11761 tacagctttg atagatataa taataatgat tttggtttct ataataatga tttcgaagtt
    11821 ttctaaaata gataagagat ctggatacat aaatttgatt tatttaatgt ggcttgttta
    11881 tgcaggattt ttgaactatt ttatatggtt tattaataaa tagaatgtaa agattttgta
    11941 aattaccctg tacttttaat aatatcataa caaacgaaaa ataaatagac tattaaaaat
    12001 aatcattttc atatacaata atattaattt aaatacaata aatttataaa aattgtattt
    12061 aaagattaac atattaaagt attagaattg gaaaggcttg aagggagagg tttttatgca
    12121 aataatagta tctgggagac aaatgaaatt aactgacgga ataaaaggat acgtagatgg
    12181 aaaattaagt aggttagaaa aataccttga tcctgaatca gaagtgaaag taacagtaag
    12241 tgcgaaaaag gatagacaaa aagtggaggt tacaataatt cctattaacg gtcaaataat
    12301 aagagcagaa gatgtagaag atgacttata tgcagcaatt gatattgttt gtgacaaatt
    12361 aagtagacaa gttgtcaaat ataaaactaa agttaaagac aaagttcaaa ataataagag
    12421 cataagattt gaaaatcttg actttataga taatagcagt gaatttgatg attatgatta
    12481 tgatgatgaa gaagatgaaa atatagtaat agaaagaaga aagaaattta atgtaaaacc
    12541 tatgagttct gaggaggcga tacttcaaat ggaactagta ggacacaatt tctatatgtt
    12601 tagaaaccaa gataattttg aaataaacat agtgtataag cgtaaagctg gtggatatgg
    12661 tttaatagaa caagattaat aaaaattgta tacgttt
//
LOCUS       DAYAOX010000109        12542 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.109, whole genome shotgun sequence.
ACCESSION   DAYAOX010000109 DAYAOX010000000
VERSION     DAYAOX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.109"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            77..988
                     /locus_tag="ACGI57_002317"
     CDS             77..988
                     /locus_tag="ACGI57_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717393.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDYDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDE
                     ELYNINEQESEYNYNEKNTKTRAKKKR"
     gene            1103..1283
                     /gene="ssrS"
                     /locus_tag="ACGI57_002318"
     ncRNA           1103..1283
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACGI57_002318"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1466..2683
                     /gene="cdeC"
                     /locus_tag="ACGI57_002319"
     CDS             1466..2683
                     /gene="cdeC"
                     /locus_tag="ACGI57_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HFG9717394.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(2784..4106)
                     /locus_tag="ACGI57_002320"
     CDS             complement(2784..4106)
                     /locus_tag="ACGI57_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFG9717395.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(4157..4951)
                     /locus_tag="ACGI57_002321"
     CDS             complement(4157..4951)
                     /locus_tag="ACGI57_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HFG9717396.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            5061..6506
                     /locus_tag="ACGI57_002322"
     CDS             5061..6506
                     /locus_tag="ACGI57_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9717397.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            6535..6744
                     /locus_tag="ACGI57_002323"
     CDS             6535..6744
                     /locus_tag="ACGI57_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HFG9717398.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            6764..8158
                     /locus_tag="ACGI57_002324"
     CDS             6764..8158
                     /locus_tag="ACGI57_002324"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFG9717399.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVIWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            8398..9150
                     /locus_tag="ACGI57_002325"
     CDS             8398..9150
                     /locus_tag="ACGI57_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9717400.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            9176..9589
                     /locus_tag="ACGI57_002326"
     CDS             9176..9589
                     /locus_tag="ACGI57_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9717401.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            9593..10336
                     /locus_tag="ACGI57_002327"
     CDS             9593..10336
                     /locus_tag="ACGI57_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HFG9717402.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            10336..10806
                     /locus_tag="ACGI57_002328"
     CDS             10336..10806
                     /locus_tag="ACGI57_002328"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9717403.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            10823..11581
                     /locus_tag="ACGI57_002329"
     CDS             10823..11581
                     /locus_tag="ACGI57_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9717404.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            11565..12407
                     /locus_tag="ACGI57_002330"
     CDS             11565..12407
                     /locus_tag="ACGI57_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9717405.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
BASE COUNT         4982 a         1392 c         2249 g         3919 t
ORIGIN      
        1 ctttaaaaat ggaacattaa aatatgaaaa acatacttta atatagggca attatataat
       61 ataaggggga attgtcttga ataacttact atttttgatt ggagtgattg cattgagtaa
      121 tggtaatatc tctatatttg atgataaaaa taaatcaaaa ggaaataata gagttagaaa
      181 aaacaaaaaa aatgcaaatg ataaactttc acagtctaca catggcaatt taaggtttga
      241 tgcaaatgat ataaaaagag gtttaaggct catggattta agtgaagaag atttagaaat
      301 gggaatggaa ataataacaa gaactaaaaa atacatgtca agagatgaaa gaaaaatact
      361 aataaaaata gaaagtgttt tagatttagt tagaggaata aaaaaattaa ataatataga
      421 tatggtagat acagaagatg aaacggattt tttcagaaaa atggatagtg atgataaaaa
      481 gaatatgatg ataaaggaaa ttatagatgt attcccagaa aaaagaaaaa attcagttga
      541 aaaagctata ggaatgaaaa agaagataga tttatttgca gaattatttt taccagatga
      601 ctttggtgaa ggtggattta gtttgagctc tttggcaaat ataaacaatc ttggtagtat
      661 gaataatctc aaattgttag gaagtctttt aagaggtgat gatagtaata atgatgatga
      721 agacaataat gatgagttaa attatgaaga cgaagaatta gattgtgaag acaaagaatt
      781 ggatgatgat tatgatgagt taacagacga aggtgaaatg gaggataatg aagatgaatt
      841 agtagaagat aagaacacag gctatgaaaa tatagaaaat gatgaagata tagtatatga
      901 tgaggaacta tacaacatta atgagcaaga atccgaatac aattataatg aaaaaaatac
      961 taaaactaga gctaagaaaa aacgataatt aactaaatac ataaagaaaa aaggaagaaa
     1021 aagtaatgat agaataagat tatctcaatt actatataaa accactttaa aaaattcaaa
     1081 atttattata taataataat atgaaatcct gaaacatacg ataaggcttt tttaaattca
     1141 acctacaaat gaaatacggg agaccgtgtc tggtaaatta taacatgcct agttagtttg
     1201 gacgttaaag gttactggca agtcacccac ctgggagtgc ctagggtgtg aatttactta
     1261 gccaccggtg tttcgggaca aaaaaagtgc tactatttca aatagtagta ctttttttat
     1321 taatatgcat ttttctacta aatacgccta attattgata aattcaaaaa gcaagtaaca
     1381 attaagatta taaaagcata gtattaatta ggcttcaaaa agccaaagac ttaataagaa
     1441 tcgcttacag ggagggcaat gaaaaatgca agattataaa aaaaataaaa gaagaatgat
     1501 gaatcagcca atgtctacaa tgaatgaaga agaagtgtat acagatgaaa taaattcaga
     1561 agacatgaga ggttttaaaa aatcacacca tcataatgga tgtaatactg ataataagtg
     1621 tgagtgccat gatgattgca atccatgcaa cccatgtaat ccatgtaaac ctaacccatg
     1681 caatccatgc aaacctaatc catgtgatga caattgtgga tgccatgaca attgtaaatg
     1741 tgattgtgaa ccatgtgaaa tggattcaga tgaatgtttt gaaaacaaat gtggaccaga
     1801 atgctgtaat cctatatctc caagaaactt ctctgtatca aatgcagtgc catttgcaat
     1861 agaggctaat agaatatttg atactatgca attccaaaca tttacagatg caacaggacc
     1921 aaatggagag ccattaactt ttgaaacaga agtagtagaa gtatttggtt cagttccaag
     1981 tgcaggtcaa gcaagtgtaa ctatagaaaa aatatgctta agtaatgatg gaatcgttat
     2041 agacacagga atgacaactt tagaagattt cgatttagac ccattaggag atatagtagg
     2101 aagaaactgt gaaacaactt ttgaatttgc agtttgtgga gaaagaaacg ctgagtgctg
     2161 tagacaagga aaaggcaaat cagtagctta taaacaaaga ggattaactg tagcagttcg
     2221 taatttagta ctagagctaa gaggtagatg tggatgtaca gagttcgttg cattagcttt
     2281 cccagcagtt agagcaggag gtggatgtaa gagaagagtt gattatgtag aatttacttt
     2341 taacacactt tcagcaccaa tatgcttgcc agctgacgga agagctgtta ctttaagaca
     2401 agaatatcaa actaacttaa ctgtagattg tataggaaaa tctatattaa aattagaatg
     2461 caacgaatgt tgtgaacctt tctatgaatt aattatacca aatgatatag atttagtact
     2521 ttgcttacaa gaaacagtta gcacattaat aagtgaacaa atagtagttt tagcatcacc
     2581 aaatccaatc caaccaagac ttgttgatac tttctctaaa gtatgtgatt tttcgcaatg
     2641 tggacctaat catggaagtg gaaagccaag ttgccacaga tagataggta agatatatag
     2701 acagtttaaa ttataaataa aaatcatcta aggaaggcta ccaaagtgta taatttacat
     2761 tggtagcctt cacgttttta taactagcgt atagatttta gtgatttaaa aagcactata
     2821 gtatgtaata gacaagtaaa gattgatact atcgtcattg tgtatatgta tgcataaata
     2881 ttaagtcctg gaattggaag aaatacatat aaagctatta gttgaattaa catgccagta
     2941 atagtattta tacttgaagc taattctttt cttattgaat gtaaaatgcc agatagagtt
     3001 tgatttaggg acataaatag tggaactaaa aacatagctt ttagatattc tcctgcaagt
     3061 gtatttttaa atactaatat acataaatct tttccaaaga aataaaaaaa tattgatgat
     3121 agtatgccta caaataaagt aagaagtatg gagtatttga tttttttaat tacactctta
     3181 cgctttctta gagccatttc ttgtgaaata gttggtatta aatttacaat aagcgctgaa
     3241 ccaacagtaa aaggtaaaaa tataaaaggc attaccatcc cactgacaat accatacata
     3301 cttaaagatt gttgataagt cattcctgat aatgctaatc ttgatggtat catcatagaa
     3361 cttatagatt gcaaaagtat gtttgacatt ctattacaag ttatgggaat agacattttt
     3421 aatgtttcca tagaagaatt ataaaaatct tttatgctta taatatacct attgtcaaat
     3481 gatgaatctc gccataagca aataagcata aatataatat tactcaactc accgattgat
     3541 atacctaata gagctatata acaattcata gatttattat ttatgtacat aactagtaaa
     3601 aaaacaaata atattttacc aatttgttca atgatttgtc caatagcagg gatttttaca
     3661 ttctttatgc catagtaata tcctcttagt acgtttgaaa gagttattat aactatagca
     3721 ggacaaacag ctagtataaa tagatttaat ttagcatctt ttaaaaattt aagtgataaa
     3781 aaagaactat tgaaagatgc aactatagat ataattagtg ctacgaaaaa tgatatatat
     3841 aaagttgata taaataaagc attagtattg tgtctatcgt ttaaagcttt tctctttgca
     3901 actagacaac taagtgcagt aggtatacct gttgttgtaa cagctaagca aatcatcaaa
     3961 aagttaaaaa tcatatgata gatacctaaa cctgtttcac ctagtgctct tgataagaat
     4021 attttatata aaaaaccaaa tattcttaca ataaaattgg atataaataa aataactgtt
     4081 gatagtacta aataaggtac tttcaaaaaa atcacctcct tatcttttaa atatattcac
     4141 aaggaggtgt aaaaatttat aattttataa attctggttt caatttatta aatttagata
     4201 catatttaaa tcccatattt tttagtaatg aatatatgta gtcaaactca catgcaactt
     4261 gacttatatg atgtgaatct gaacctgtgg ttatgatttc ccctcccaag tctttataca
     4321 tttgaagtat atacttagat ggggttaggt ctggaagatt atatctataa caagaggtat
     4381 ttatctcgat acctttgcca tcataaatag cttgtttaag agtttcttca attatatcag
     4441 aaaatagtct atcatctaaa atagtgtcat aaggtgcata ccttttaatc aaatctaaat
     4501 gacctaagac agagtaatct ttataattct taacaatatt atataaataa agatagtaat
     4561 tttcataaac ttcatgttga gttttatctt taaaataatt accaaggtat aagtccattg
     4621 tatctatagc atggatagaa catattataa agtccaatgg atattgatgt gcttcctttt
     4681 tgtatgtatc cattaattga gtttgaactc caaattcaat tccttttttt attgatattt
     4741 tatctttata tttgctttga agagtctcta aactcttaaa ataatcttcg tagacaattg
     4801 aaaaagaatc atcagcatat acatcatagt caacatggtc tgtgaaacaa atttcctcca
     4861 aaccgagttc aatagatttt tttaccattt cttccattgt agattttccg tctgtagaga
     4921 aacttgagtg catgtgatag tcgtaaaaca taaatatttc ccctttttat aaaaattttg
     4981 gtattatata tacatactaa ataatatcaa atatacataa aagcatcaat aaaatataaa
     5041 tacaatgagc ggtgataaat ttggaaaaat acagaataga tttaaatgag aaaaaatcaa
     5101 ctaaatatat acagatattt aatcatataa aagaattgat aataaataaa gagttagtag
     5161 aacatgaaaa attgcctcca ataagaaaac tatcaaattt tttacaagta aataacacta
     5221 ctatagttaa ggtatacgag cttttagaaa atgaaggata tgtatataaa attataggga
     5281 gtggtacttt tgtatcaagt ttaaattctt ataaaagcgg tatagataac aggaaaaata
     5341 agattattgt gcaaagagaa gatttaattc attttgacaa tgggaatcca tctaatgata
     5401 tgtttcctat cgattcattt aaaaatgctg taaatatggc attatctaaa gatggtagtt
     5461 caatatttga atatgatgaa ggtcttggct ctgaagaatt aagagaaaaa atggtagagt
     5521 atctaagtga tggaaatata aaaactacta aggaaaatat tcaaataata tcaggtgctc
     5581 aacaaggtat agatatagtt tgtaaaggac ttataagtta ttcagatgtt gtttttatgg
     5641 aagaaccttc ttacaatgga gcaattgaag tgtttaaaag tagaggtgca aaaataatat
     5701 ctatacctat gttagatgat ggaattgata taggtatttt aaaattaaag ttggaaaaaa
     5761 taaaaccaag acttatatac attatgccta attttcaaaa tccaactgga atatcttact
     5821 caacatataa aaaaaagaaa ttgatagaat tggcagaaga acatgatttt tatataatag
     5881 aagatgattt tataagtgat tttgtttttg aatcaaagga caatagaaca attagaagtt
     5941 atgatgataa aaatagagta gtatatataa aaagcttttc aaaaatactt atgccaggtc
     6001 ttaggatagg aattgtagaa atgccaaatg agttgttaaa aaaagtttta tgggctaaat
     6061 attcatcaga tatatcaaca ccaggtttaa ttcaaaaatc aatgtattac tatatgaata
     6121 attttgattg gaagaattat ttgagtcata tagaaaagat atatactgat aagtataaat
     6181 tggcaaaaaa cttgattaat gacaaattga gtggtaaatt aaaagttaga aaatcttgtg
     6241 gaggaataaa ctttttttta gaattaccaa gaggatatac atctcaagca tttacaaatt
     6301 ttatgttaaa caaaggagtg tcaatgcttc cagggactta tttttttgac aatctagtgg
     6361 atgatagatt ctttaggata aatatagctc gcccaagcat ggaagaacta gaaaagggaa
     6421 tatctataat aagtgataat atagatgagt ttttttgtga atataaaaac aagttagata
     6481 taaaaagcaa taaattattt tattaaataa atagaggttt tttaggagga aaaaatgttt
     6541 gacaagaaga aattagatag gattaatgaa ttagcaaaga aaaataaaga gggaatcctt
     6601 tctgctgatg aaatcaaaga aagagaaatt ttaaggaaag aatatttaga aaactttaga
     6661 gcccacttta gatcaagact agatagtgtc aaagtagttt caccagaaga atatgagcaa
     6721 tacatgaaaa acaataaaaa ttagaactta gtaggaggaa aatatgaact taaagtacga
     6781 atttaagaat gcttgggatt ttgaaagaaa agaaaatact attgaacaaa ttatgcaata
     6841 ttctagcaat tatatggagt ttttaagtaa gtcaaagact gaaagacttt cagtaaaaga
     6901 aattataaaa ttggctaaag aaaataacta tatttcaata gatgaagcta tggaaaaagg
     6961 aagcataagt tgtggtgata agatttatgt tataaataaa gagaaggcag tagcactttt
     7021 tgtaataggt aaaaattata tagaaaaagg tatgaagatt ataggaagcc atatagattc
     7081 gccaagatta gatttaaaac caaatccact gtatcaagag agtaatctag gtttttttaa
     7141 gactcattat tatggtggaa taaaaaaata ccaatggacg gctattccac ttgctttaca
     7201 tggtatagtt atattaaatg atggaacaaa agtggacata tctattgggg aagaagatag
     7261 tgacccagta ttttgtgtaa ctgatttatt gattcatttg gctggagacc aaatgcaaaa
     7321 gaaactatca gaaggaatta gtggagaagc tttaaatata ttaattggaa atatgccttt
     7381 agaagatgaa gaaaaagaac ctataacagc aaatatatta aagattttaa atgagaaata
     7441 caatatagtt gaagaagatt tattaagtgc tgaaattgaa gtagttccag ctggtaaggc
     7501 tagagattta gggcttgata ggtctatggt gttgggatat gggcatgatg atagggtatg
     7561 ttcatatgct gctgtaaaag ctatactaga aactgaacaa ccagaattta cttcagttgc
     7621 attatgtgta gataaagaag aaattggttc taaagggaat acaggtatgc actcaaagtt
     7681 ttttgaaaat actgtggctg aactgattgc tttagaagga gattattgtg atattaaagt
     7741 aagaagagct ttagcaaata gtaaggtatt atcagctgat gtttctgctg gatatgaccc
     7801 aaattttgga gaagcatatg aaaagagaaa ttcggcatat atgggaaatg gagtagtttt
     7861 aactaagtat acaggttcaa gaggaaagag tggatgtaat gatgctaatg ctgaatttat
     7921 gtcagaagta aagagaattt ttaataaagg aaatgtaata tggcaaactg ctgaacttgg
     7981 aaaagtagac caaggaggag gcggaactat agcccatata ttggcaaatc aaggtgcaga
     8041 ggttatagac tgtggtgttg gagttttaaa tatgcatgca ccacatgaga ttgtctctaa
     8101 agtagacata tatgaaatgt ataagggata taaggcattt tttaatataa atttataagt
     8161 aaaaatataa atgtaataag tgaataaagg ctgttgtata aacaatttaa tctgttgtac
     8221 aacagtctta tttttatgta aaaatagcaa tttgtcacac tagagaaaat aaaagaaaat
     8281 actgtcaaat taaaaattat ttaaaaaaaa attgacaacg atttcatctt gttataatat
     8341 gtaataaaga acttgttata taatgttata aaaatgttac atagggaggt gagcaaaatg
     8401 gatgaaaaga gacaaaaaaa agaaatggat ttagaagtaa attcctttgt tcctttatat
     8461 caacaactat atgacaacat aaaaaaacaa atagcatctg gcatatataa accaggagat
     8521 aaacttccat ctgaaggaga cttatgtaaa gaatttaata taagtcgtat aactgtgaga
     8581 aatgcactta atgaacttgt aaaagaagat atattatgta aaaaacgtgg taaagggact
     8641 tatgtaacaa taccagagag aatagaagca acatgtgcag gaaatagttt tactaattct
     8701 tgtcatcgta ttaatgcaaa accaagtacc aaaataatct ctgttttaat aaaaaaagca
     8761 gataagcaag tagctgaagc tttaagtatt gaactagaag agaaggtaat atgtattaaa
     8821 agacttagat taatagatga agtacctgtt atttttgaag ttgactattt tagaatagat
     8881 tacatgtttt tattaaaaga agaattagaa gataaatctt taatggaggt tatatctaat
     8941 aatataagca cattacctaa acgagtagaa aatatatttg aagtaaaaca ttcaaacaaa
     9001 gaatattctg accatcttaa atgtgcaagt aatatgccac tattgaaagt aagacaatcg
     9061 gtgtatacag aaaataatga tgtcttatat tacaatgagc aatttataag aagtgataag
     9121 tacaaatatg cagtatcagc agaaatataa tctataattt agtaggagga tgagtatgaa
     9181 aaagataata ttagcatctc atggagactt ttcaaaaggg cttttaaatg cagtaaaaat
     9241 gatagtagga gatttagcag attgtgtcag ttcgtatggt ttgtaccctg gacaaagtgc
     9301 ttcagatttt gctttagaat tagaaaaaac aatatcagaa gataaagaat gtgaatatat
     9361 aattttatct gacttatatg gagcaagtgt atgtacagca atgttaagat taactaatct
     9421 acataatgtt aggttatttt ctggaatgaa tttgaatatg gtattagaac ttcttacaag
     9481 gttttctgat gaattaacag ttgaagatat agaccaatta gtggaagaat ctagaagagg
     9541 aatacaaagc gtgacattac aaattaaaga agaagaagag gtattttagt ttatgaaaaa
     9601 aaagggaata ttatcagtat ttaaaagtaa aaaaccaatt atagcaatga tacacttaaa
     9661 aggagatact ccagaagata tctttgaaag agctaaaaaa gagattacaa tctttgaaga
     9721 aaatggtgta gatggaataa tgttagaaaa ttactatggt aattattatg atttagaaag
     9781 aatacttgaa tatgtatcaa aagctaattt atcaatacca tatggagtaa actgtttaaa
     9841 tgttgatact atgggatttg aactggcaac aaaatataat gctagttata tacaggttga
     9901 ttctgtagta ggtcatgtta aacctagaga tgaagctact ttagaagagt tttttaaatt
     9961 acaacgttca aaatgtccag cgtatttaat tggaggcgta cgttttaaat atcagccagt
    10021 attatcagaa aatgatgttg aagaagattt aaaaatagga atgacaagat gtgatgctat
    10081 agcagttaca gaaaatgcta caggtcaaga aacatcaatg gaaaaaatag aactgtttcg
    10141 taaaaattta ggagacttcc cattggttat agctgctgga gttacattag aaaatgcaaa
    10201 aaaacagctt aaattaggag atatggcaat aataggaagt tactttaaag ataattacaa
    10261 agattttggg gatgttagtg tagaacacgt aaaaacattc atggatgaaa tcaaaaaaat
    10321 tagggaggaa ttataatgat taaattagta agagtagacc acagacttat acatggacaa
    10381 gtagcattta catggacaaa gtttttaagt acagactgta tattgatagc aagtgatgac
    10441 ttattaaaag atgaattaag aatggcagga cttaaaatgg ctaaaccatc taatgttaag
    10501 ttagtaatga aaagcatagc agattctata aaagcactta actcaggtgt tactgataaa
    10561 tacaacttat tgatactttg tgaatctgta gaagatgttt acagacttgc taaagaggta
    10621 aaagctatta aatctataaa ccttggtgga acaaaatcag atgataatcg tgaaaatata
    10681 tctaaagcag tgcatgtatc aaaagatgat ataaagatga ttaaagagtt agattcagaa
    10741 ggcgtaaatg tatttgtaca attagttcct gatgatgatg ctacaaatgt aatgaaatta
    10801 atataatttt agggggatga aaatggaatt tacacaagtt attttgataa ctctaattgc
    10861 attctttgca tatatgcata gttttgtagg ttctacaatg cataatagac ctatagttgt
    10921 tgcaccactt gttggtttag cactaggtaa tcttcataca ggtatagtaa taggttcaac
    10981 attagaatta gtatttatgg gtgcatttcc tgttggggca agtaatcctc ctgattttgt
    11041 gtctggttct attattgcca cagcgtttgt aatattaacg ggtcaagatg tatcagcagc
    11101 agttgtactt gcagttccta tagcaactct agtattgctt atagataact tcttaatgac
    11161 agtagtactt acatggggag ctcatattgc tgaccgttat gcagaagagg ggaatattga
    11221 aggcgtagaa cgtgtgcaat tattatttgg tataggaaat aaattaatat tagcaataat
    11281 agtaggtata ggattttcat taggtgttcc agtaatagaa aaaatacttt catttatacc
    11341 atcatatgta actcatggaa tggatgttgc agcaggcgtg attccagcaa taggttttgc
    11401 catgttagct agaatgatgc ttaataagaa aacagtagca tttttattat taggatttat
    11461 attggttgct tacctaaaca taacagtaac aggagttgca ttgtttggat tagcaatagc
    11521 tttaatatat gttaactttg taggagagaa ggaggtaata gtagatgaca acgagttcta
    11581 agaaattaga gacaatttca ccagatagta aaataacgag gaaagacttt tggaaatgtt
    11641 ttagaaggtc attgacatta gattcatctt ggaactacga acgtatgcaa aatatagcat
    11701 atgcatacat gatggcacca ataatccgta gattatataa agatgataaa gaaaagaaat
    11761 ctaaggcttt aaaaagacat ttagaattta tgtcagttac tcctcatata agtactcttt
    11821 tggtaggtat atctggagct atggaagaag aaaatgcaaa gaataaagaa tttgatgcta
    11881 atagtataaa tgctgttaaa tcaagtttaa tgggtccagt ttctgggata ggagattcat
    11941 ttttctgggg aacattaaag cttatagcag ctggggttgg tatagcttta gcttctcagg
    12001 gtaacataat gggaccgatt ttattccttt taataataaa tgttcctcat tttattatac
    12061 gctatatatg tctagataaa ggatttaagt atggaactca attctttaaa gatgtgagtg
    12121 gttcaagtat agtttcaaaa gttatggaag cagcttcaat gttagggctt atggtaattg
    12181 gaggtatgac tgcatctaat gtaatgttaa aacttagtgt taatgtaggt agtggagaat
    12241 gggcagagcc tattcaaact tacttagacc aaataatgcc atgtatgctt ccagctatga
    12301 tattcggtat tatgtattgg ttattaggaa aaaaggtaaa gacaactacg atattaattt
    12361 cagttatgat tatttgtata gtattggctg ctattggagt agtataaata aaaaaatccc
    12421 aagttcattt gaacgaggga ttttttgact aaattaaata aaaaagaaag atggttcata
    12481 ataatttata agcatttatg tgatagttat aattagctta ttaattagtt ataaactcaa
    12541 aa
//
LOCUS       DAYAOX010000110        12494 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.110, whole genome shotgun sequence.
ACCESSION   DAYAOX010000110 DAYAOX010000000
VERSION     DAYAOX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.110"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            108..1031
                     /locus_tag="ACGI57_002331"
     CDS             108..1031
                     /locus_tag="ACGI57_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9717406.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(1203..2345)
                     /locus_tag="ACGI57_002332"
     CDS             complement(1203..2345)
                     /locus_tag="ACGI57_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFG9717407.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELRINK"
     gene            complement(2363..4117)
                     /locus_tag="ACGI57_002333"
     CDS             complement(2363..4117)
                     /locus_tag="ACGI57_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HFG9717408.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            4253..4459
                     /locus_tag="ACGI57_002334"
     CDS             4253..4459
                     /locus_tag="ACGI57_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717409.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(4464..5387)
                     /locus_tag="ACGI57_002335"
     CDS             complement(4464..5387)
                     /locus_tag="ACGI57_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717410.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(5641..7341)
                     /gene="argS"
                     /locus_tag="ACGI57_002336"
     CDS             complement(5641..7341)
                     /gene="argS"
                     /locus_tag="ACGI57_002336"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFG9717411.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(7803..8216)
                     /locus_tag="ACGI57_002337"
     CDS             complement(7803..8216)
                     /locus_tag="ACGI57_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFG9717412.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(8284..10662)
                     /locus_tag="ACGI57_002338"
     CDS             complement(8284..10662)
                     /locus_tag="ACGI57_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFG9717413.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            10981..12432
                     /locus_tag="ACGI57_002339"
     CDS             10981..12432
                     /locus_tag="ACGI57_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HFG9717414.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
BASE COUNT         3961 a         2165 c         1500 g         4868 t
ORIGIN      
        1 aattatatct atatcaaata tctatagtaa atacttaact atacttaaaa agcatataaa
       61 ttgatataat tatataatag ataagttaat attgaggagg gataattatg gaaataagag
      121 tattaagata ttttttagca attgcaagag aagaaagtat atctggtgcg gcaacctttt
      181 tacatataac acaaccaaca ctttctagac aaattatgga acttgaagaa gagttgggag
      241 ttaaattatt ggttcgagga aatagaaaaa tcaccttaac aaatgaaggt aggcttttac
      301 ataaaagagc agaagaaatt atatcattag ttgataaggt tgaattagaa tttgacaatc
      361 ccaatgagat tattagtggt gatatatata taggaagtgg tgaaactgaa gctatgcgac
      421 ttatagctag aactgcccat cattttcatc aaaaataccc caatgtaaga tatcaccttt
      481 ttagtgggaa tgctgatgat gtgactgaaa aattagacaa gggactgctt gattttggta
      541 ttttaattga acctgcaaat atacagaagt atgattatgc acaaattcct gctactgatg
      601 tatggggttt actaatgcgt aaagatagtg cccttgcatc acaaagcaca ataaatccaa
      661 aagatttatg caatatacct cttattattt caagacagac acttgttgat aaagcaattt
      721 ctaaatggct tcaagacagt aatgaaaaac taaacattat tgcaacatac aatttagttt
      781 ataacgcttc tcttttggta gaagaaggtc tgggctacgc tctgtgttta gatagactcg
      841 tcaatactac agaaaacagt aatctttgtt ttagacctct atctcctcct ttagaagcta
      901 aattaaatat tatatggaaa aaacatcaaa tattttcaaa ggcagcagaa aaatttttac
      961 ttcaaataca aaaagatttt tcttctatca tgttggatac atctaagttg gatacaccta
     1021 aagaaaaata aatcataata aaaggtgaga gtgtctcaaa atgaactttg cactgtataa
     1081 ggcactcttt tttatacaaa gtaaaatata tgttcatcat ttcagcacta aaaaagaggc
     1141 ttatctctat tttgagatag ccccacttaa ccaataaaat taaatacaac ttaacaatat
     1201 ccttacttat ttattctaag ttctcttaga caagcaatag taatttcatc ttgtggattt
     1261 aattcataag ctctttctat atagtaagtt gcatcatcaa tatcaccatt atttaaataa
     1321 gccattccta aattgcaaag tatttctggg tcttccttta tcttagccgc ttgttcaaaa
     1381 tactctattg ctttatctaa attattctta taagcctcac aaagccccaa ttccactata
     1441 gtatccactt gattaggctt tattatcaat atcttctcaa gatacatctt agcctgctct
     1501 atttcgccca tacccttata tccaagtgct atcataaaca gcaagttcca ccaatccata
     1561 tgttcttctt ctaatggaag taacttctct aatccttcct taaacttacc ttggaataca
     1621 agtgtatatc cttcttcata ttgaacttta aagtccatct tgcccagatt ctcttgtacc
     1681 tcagctacta aatcagcatc taaccctagc cttaacgctt cttcccaagt cagcttagtc
     1741 tttaaatatt gaccttgatt ataataatgg tagcctaagt gataataacc taatgcaaaa
     1801 ttctcatcaa catcaattac cttttcaagc ttagccatgg cttcaagtaa gaatgcattc
     1861 attgccttat catccatatc tttttgatac tgtttagcta tctcttggca aacaatcgca
     1921 tagttataca ttgcatctaa atcatctgga tacattgtta taaatgcttt tatatatatt
     1981 aaagaatctg tatgttcacc aatctcaaag tattttctcg acatatatcc catatacgat
     2041 ttcaaatcca aatcaatatc ttttgataaa gcatttaaaa acttctcata ctctgcattg
     2101 tttttaaact tactatctat tccaattata tatatcattg catctgctat agacattgaa
     2161 tttaacttat cttgagcagt cttttctttt atccctttta ctaatacctc attttttata
     2221 ggtacatcta atccaccttt tggcacttca tagcttttta atttaaactc tccaccatgt
     2281 tttatagtta taaaagctaa ttcttctgct ttttttagca aatatttttc tatcttaaaa
     2341 tccattttat cctcctaaat acttaaatct ctactttatg agttttagat ttctcaaata
     2401 tttttttatc atcatactca aataagacta cattttctac ttctcttatt tctgtattta
     2461 tatcattttt taaatccaca acattaaatc taaatggctc aaaatgaaca tattttatta
     2521 tatgtttagc tggtttatca acaaaactag gtaaatatgt tgataagtta tcattatctt
     2581 gtaaaaatac atggcatcta ttcttaaaat catccacatc tagcttatta aaaaactgtg
     2641 gtatatttga tgttttaccc agtgatatat agtcatattt taatatatcg ttgaatacat
     2701 cattatctat gttgattttt tcattataga aatccatcaa tattttatat aactgattct
     2761 ttccttgtgc caaatcaaag tagccatttt catcaaaata cttggcaaaa acctcaaaga
     2821 atttaaaagg gcttttttta taaaatctgc ctataatata tctcatagat aacacaaagt
     2881 ttttagaatt gtagtatctc tccagtatat cttctatatc ctttagcttc aaagtttctt
     2941 catagcttat ataattattg tataaaaact catatggagc ataatcctta tatttaaaac
     3001 catgctcatc tgcaactttt ctcatacctg tccctttaat catctttaaa aagcctaatt
     3061 gcaaatgctc tatacctaga ttaaatacat cattaaaaga attttcaaag ctcttataat
     3121 cttcataagg cagtcctgca attaagtcta agtgctgatg tatatttcta taagatgcta
     3181 cagtttgtac aacatgagac aactttttaa aatcatccct tctacctact gcatcaagtg
     3241 ttttttcatt cgttgactga acacctattt caaattgaaa aagaccttct ttacaatcag
     3301 ccaaaaactc aagcatctta tcatctatca aatacgctgt aacttcaaaa tggtaagttg
     3361 tatatccatt atcatttttc ataaggaaat ccatgatttc catagccact tttctatttg
     3421 cattgaatgt cctatctata aactttatct gtgatactct tgcatctatt aaagctttta
     3481 aatcactctt tatcctgtct aaactaaaat accttagacc tggaatagta gatgataaac
     3541 agtattggca gttaaaaggg caacctcttg atgattcata atacactatt ctattttcat
     3601 attcttttgg attcaagttt tcataaggac taggaatctc atccaaatct tgaagtaatt
     3661 ccataggctt attcactatt atctcattgc cttgtctata tacaagtcca tttacatcat
     3721 ttatttccat ctttccttgc aagtgaagta ctaagtctct aaaaattaat tctccctctc
     3781 cataaagtat ataatccaca aaatcatact ttttcattgc ctcataacta tcataactca
     3841 cttccggtcc gcccaaagct attttagtat taggtctaat tttttttaga ttatcacaaa
     3901 gtttaactat atctcccaca ttccagatat atgttgagaa taatattata tcatattcat
     3961 ttttatatat atcctttaaa atatagtcca aatcgttatt tatcgtgtat tctttcatat
     4021 taacttctat caaatctctt acaaattctt ttagatatct tatagccaaa tttgtatgaa
     4081 taaacttgga atttaatgtt gttaataaaa ttttcaaata tttctcacct ctttttaaga
     4141 ttatactgtc tttttgtacc ccttataggt taaaatagta tatgtaaagt ctaaataata
     4201 tctatataat taatttatta atttcaagta atgtgaggaa aggaagtttt ttatggaatt
     4261 aattgaaaaa gtatctatca actctttatc aaaaagagat ttactactta ttataaagtc
     4321 tcttgaatac actaatgaaa atactcaaat tagtgatttc attgacctta ggaataatat
     4381 agtaaaggaa ttatgttttt taactgacac taaggaaaaa gattttttag attatctaga
     4441 aaaaaatagc acactctaat aacctaaacc tttttaaact ttaatatata aatacctgat
     4501 ttagaattta tttttatgct taatatatta tcattaaagg ttttcgacat aactgttatt
     4561 tgattaaata tagggtctat tatagtatta ccttcttcaa gtcttataag acttcttggg
     4621 tctatgcttt tattatatgc cttaactcca attttatatt ttctcaattt aacaacatat
     4681 tcggacatag aaaaaataaa atctatatta tcttcattta atttactatc ttcacccaaa
     4741 atggataata tagatttttt tatgctatta attccttcat tttctacagt tctatcttcc
     4801 atatatacat catatagaac atttaaaaca tttaataaaa tactttcttc atcataatct
     4861 tcagatttca aatcaatgta cttatcaatt aattgtattg ctttaatcct tgccatctga
     4921 aactttacaa catctgattt gtctaattct atagaaaatc ctattatctt gtctttaata
     4981 ctctgtagta attcgacata ctctatttta ctattattta taagattgtg cataatggaa
     5041 ttatacacca cagaacctaa taaataatca aataaaagtt cttctttttt aaaataaata
     5101 acatactttt ttattagttt aataacctca tcttcacttt tttgaatatt agtatttgat
     5161 aaaattagtg gtaataaaat atttaaaaaa ttgatatttc ctattttttc tccataaaat
     5221 ctatagtaaa taagttcatc tctcagtcta ttttcgtcta tatacacaca tctttgcgtt
     5281 ttatcatcta ttaaggattt tgtatttatt atagttaaat ctttaagagc cctacttgca
     5341 ctactaaaag aagatgtgta gacttttttt agtaattcgt cattcataaa tatatccttt
     5401 ctgaaaaatt gaatgtaaat aaattgttat taaaaaaaaa gagacagccc gtttaagaag
     5461 ctgtcatata tagggggata gtagtgtaca aaaatcattg taatctacta tctatatatt
     5521 attatacgat actacttttt tatatgtaaa gcatcagttt tcgacatccc tatattttac
     5581 agttatacaa attgcgtact aagttcaatt ttattctttt tatttcaaca ttttatcatg
     5641 ctacattctt tcaggagcgt gcattcccaa caatttcaga gaattttcta aagtttgttt
     5701 tgttgctaaa actaatgcaa gtcttgcttt tcttatttcc aaattttcta ctaaaattgg
     5761 attgtcatga taaaatttat taaacgcttg agctaaatct agcataaatc tagtcactat
     5821 atgtggctca tttttcttta gagcaagtaa aatattctta ttgaatgatt ctataactct
     5881 aagaacttct gcactatctt catcagctaa tagactataa tctatatcag aagtcacttc
     5941 tacttctgct tttcttaaaa ctgcacagca tctagcatgt gtatactgaa catatggtcc
     6001 agtctcacca tcaaaactaa gcgttctttc ccaagagaag gtatagtctt taattctact
     6061 attagataac tcttggaata ctactgcccc aacacctact tgcttagata tttcatccac
     6121 atttttagca tttgggtttt tagctagtat aatttcctta gttttatcta tagcttgatt
     6181 taaagcatct tctaaaaata ctactcttcc ttttctagtc gacatagtac cttcttcaag
     6241 tgcaaccata ccaaatccaa catgaattaa atcttttgcc caatcatatc ccataagctc
     6301 aattacctta aaccattgtt ggaaatgtaa gttttgttga gaaccaacta catatataca
     6361 cttgtcaaaa tcataagttt cttttctata tattgcagca gctaaatctc ttgtcatata
     6421 taacgttgag ccatcattct tagtaataag tgctggtggc attttatact cttctaaatc
     6481 aacaattctt gctcctttag actcttttaa aagatttttc tcttctaata gttcgataac
     6541 tctgttcatt ttatcagaat aaaaactctc tcctgctaaa gaatcaaatt ctatatctaa
     6601 aagtttatac actctgttaa actcttttaa actctcattt ctaaaccatt gccataattc
     6661 ttgtgcttct tcgtcaccat tttctaattt attaaaccat gctcttgctt catcttccat
     6721 ttctgggtgt tgttcagctt catcatggaa tcttacgtat aattttagta attctggaat
     6781 tggattagac tctactactt ctttttctcc ccacttttta aaggccacta ttaacttacc
     6841 aaactgagtt ccatagtcac ctaaatgatt tattcttata gtcttgtatc cttgcgaatc
     6901 atatatctta tataaagcat tacctataac tgtagttctt atgtgcccta tatggaaagg
     6961 ttttgctata tttggagaag aatattcaac tattacagtt ttatcttttc caaaatcact
     7021 atgtccgtaa ttttcttttt catctaaaac ttttttaatt acagtttctg ctaattgaga
     7081 tttatttaca aagaaattta catatccgcc taattgtatt acttttgtta tctctccact
     7141 tggttctata ctttccgcca attcacttgc tatcatgttt ggagcttttc taaacacttt
     7201 ggctaattta aagcatggaa aagcatagtc ccccatatcc ttatttggtg gaacttctat
     7261 aagtgcctct atctcttctt tcgataaatc ttctattttt tctttaaggc aatttgatat
     7321 tgccacctta aaatcttgca ttctgttgct cctctctaca ttgttcctgg tttatattgt
     7381 gcttatctct ttatgttaaa tcaaatttcg tgttttttca agtatttcat tacagtgtaa
     7441 aacactttag taattatgtt ttctgtatat attaaaaata ctataattgc agtttttaat
     7501 cacactatta ttgatttttt ataagacttt tttctatttt attgtttctt gtactatcca
     7561 gttgacttta tatacttttt ttaatctttt atctattttc atacgtcaat gctaatttta
     7621 tatacttttc ttttaatctt ttattaattt ttatacatca atgttaactt tatataagtt
     7681 tttttaatct tttatctgtt ttcatacatc aatgtcagct ttatatactt tttttacttt
     7741 tttatctatt tttatacatc aatattagct ttatatactt tttttaatct tttatctatt
     7801 ttctataaat ctttttcact cttaactttt attccttctt cttctaatag ccttgctgtt
     7861 atacctttcc ctggtatctt tttacctgaa aaactaccat catatattag attacatcca
     7921 caagaggggc taccctcttt tagtattgct tcgctacata catataaatt tgctattttt
     7981 agagtttcat tagcccctct tacaaagttt tctgttacat ccttattatc tttagtaatt
     8041 acttttccat caactatctc agcaggtggt cttggtgtag atagaccacc taactgctct
     8101 gggcatacaa gtgtaaattc tttgcctttt aaatattcct tgactttttc attatcatta
     8161 ttatcgccac tatatttaca atttattcct attaaacatg cagataccaa tatcataaaa
     8221 atgtgctcct atacaaattc ttaatatatt ctcatacaaa ttactaatgt attcttatac
     8281 aaattatttt aatttaacaa ttgttactcc tgctccacct tctccatatt gaccacctct
     8341 ttgtgatttt acatgtctat ttcttcttaa aatgtcttgt agccctgctt ttaatactcc
     8401 tgtacctatt ccatgtatta ctgttacaga ctctaaacct gccacatatg catcatcaag
     8461 atacttttct acttccatta tagcttcttc taggtttaac cctctaaggt caacttcatt
     8521 ttttacacta ccagattttg accttataat gtttctcgtt gatttagtta catttgttga
     8581 aacatctttg cgtgtttttt gtaaagactt aaaaggtaga gtcatcttca ttataccaat
     8641 ttgcactact gcttcttttc tctttttatc tactgaaact acactaccat tttgatttaa
     8701 agttattacc ttaacttctt cacctggctt taagtctttt atctccttat ttgatacctt
     8761 tggaactatc atacttttta ctgttggctg aagacttccc atagagctgg tcaattcttt
     8821 tctaagttct tctatcttac gattcttttc ttttgaagct ctttcttgtt caagacttct
     8881 aagttctttt attataatat ctacttcttc tttagcttgt cttataattg aaaatgcttc
     8941 tgatttagct ttttcaatca tcttatctct ttgactgacc aatttttcaa gcttttcgtc
     9001 atactctact ttcagttttt caatctcttc ttttaatctt tcagcttctt ctctatcttc
     9061 tactgctttg attctattct tttcaacatt ctgtaacaca tcttctaaag ctatgttttc
     9121 tgtatttata tattctttag cacgtgaaat cacataatca cttagcccaa gttttctaga
     9181 aatctcaaac gcatttgatt ttccgggaac acctataagt aacttatatg ttgggcttaa
     9241 agtttctata tcaaattcaa ctgctgcatt ttctacacct ggtttagtaa gagcataatt
     9301 tttaagctca ctatagtgag tggtagctat acatttagct cctacagaat ttatatcttc
     9361 taatactgct attgcaaggg cagctccttc aacagggtct gttcccgctc ctaactcgtc
     9421 aaatataact aaagagtctg ctgttacgtt ttgtaatata gatactatat tcgtcatatg
     9481 agatgaaaat gttgatagac tctgttcaat actctgctca tctcctatat ctgcaaatac
     9541 attatcatat acacacatac tacttccata atcagcaggt atatgaagtc cactttgtgt
     9601 cataagtgca aatagaccta cagtttttat tgtaacagtc ttaccacctg tgtttggacc
     9661 tgttatcacc aatgtatgga agtctcttcc taaataaatt gtatttgcta ctaccttctt
     9721 tttatccagc agtggatgtc ttccattttt tatgtttaaa tatttatcct catttaaagt
     9781 tggttctatt cctctcatct gtatagaaag ttttcccttt gaaaatgcaa agtccaatct
     9841 tccaagtatc tcttgattag aaattaagtc ttcactaact tctccaacca tagcagaaag
     9901 ctctgataaa attctttcta tttcttcttg ctcacctagt tttaactgtc tcaattcatt
     9961 gttcatctct actatagtca ttggctctat aaaaagtgtc gcaccagaag atgactggtc
    10021 atgcactata ccagctactt gcgacctata ttcagatttt acaggtacta caaatctatc
    10081 acctcttaga gaaattattg catcttgtaa gtacttttga taagtagttg aacttattat
    10141 tgaatttaat tttgacctaa tggattgatt tttttgggct attcttcttc tgatatctct
    10201 aagtgtactt gaagcattgt cagatatttc tacttcacta attatagcat tgtatatctg
    10261 gtcttctatg tccttgtata catatagaga atttgataga gattgtatta ttggataatt
    10321 aaaatcttct tcatcactac tagataagct atttcttaat cttctagcca ctcttaaggt
    10381 atctgctatc ataatcagac ttcctgggtc aagagaagca cctatataag ctctctttac
    10441 cttatcttct atatcatgaa taccttccaa ccctactgaa cctctcttta taataattga
    10501 ttgagcttca ctagtttctt ctagcataga tttcacttct acaaaatcag tatttggtac
    10561 taaattttct atatatttta gacctaaaga agatgaggct ttcttcttta acaaatctat
    10621 tattttattg tattctagaa ctcttaacga tttctcattc attttctata ttcaccatcc
    10681 tcgatatttt aactttttta tttaatttta aaaatgtatg ttttcaagca tttccaataa
    10741 aaactgcaag ttttatttct ttttcatttc taaaattatt atattttttc tcttttagat
    10801 atattttaca catattttgt attatactat gtatgtagct atctatatat tatacataaa
    10861 catgctttat ttgtaatata atctgttaaa aaatcttctt taatattttt aaaggagttt
    10921 attttagctt ataaaaaaaa atagctactt atatagataa attaaggagg aacatgagtc
    10981 atgggaaacg tattagaagg attaaaacca gaaagtgttt ttaaaaactt tgagaaaata
    11041 tctcaaattc ctagaggttc tggaaatgaa aaaggcataa gtgatttttt acttagcttt
    11101 ggtaaaaatt taggtcttga aactattcag gatgaaagct taaacataat aataagaaag
    11161 cctgctacaa aaggatatga aaattgtcca ggtgtagttc ttcaaggaca tatggatatg
    11221 gtttgcgaaa aagaaaagaa tgttgaacat gattttctta aagaccctat aaaattaaga
    11281 attgatggtg acatgatata tgctactgga actactctag gagctgacaa tggtatagct
    11341 gttgctatgg gtatggctat attagaagat aatactttag agcatcctgc actagaagtt
    11401 ttagttactg ttaatgaaga agatggtatg aatggtgcag atgcactaga ccctagctta
    11461 attaaaggtc aatatatcct aaatatggac tccgaggagg aaggatactt attagttagc
    11521 tgtgctggtg gtaaaacttg tgttgttagt ttacctgttg aatacaagga agttaaaggt
    11581 gacaaacaag gattattagt tgaggttact ggactattag gtggacactc tggtatggaa
    11641 attgttttac aaagagcaaa tgctaacaaa gcaatagcta gagtattaag tgtattgaat
    11701 gttgattatg aattagcttc tgttgatggt ggtactaagc acaatgctat acctcgtgaa
    11761 gctaaatgtg taatagctgt taataaagct gatgttgaat ctgctaaaaa acaaataaat
    11821 gacattttaa ctgcatttaa acatgagttt actacttctg accctggcat gacttactca
    11881 gtagctgaaa cttctgtaga caaagttctt actaaagatt gtaaagaaaa agttgttcaa
    11941 atgtcttgct taactcctca tggagttcaa tcagtaagtc ttgatataga aggattagtt
    12001 gaaagttcta ctaacttcgc tataatcgaa actaaagaat ctactattga atttttaact
    12061 tctgttagaa gttctgttat gtctattaga gatgaaatcg ctgatagaat aagacttttg
    12121 gctcaagctt taggtgctaa ttacgacctt atagctcaat acccagcttg ggaatttaaa
    12181 aaaggctcaa aattagaaaa aatatgcagt gaaacttatg agaaattaac tggtaaagtt
    12241 cctactgtta tggctctaca tgctggtctt gagtgtggat tattacttga taaacttcct
    12301 catgctgaag ctatctctat aggaccagat atgtttgatg ttcatactcc aaatgaacat
    12361 gttagtatcc cttctgtagc taatgtttgg gattatgtta ttgaaatatt aaaatctatg
    12421 aaccaatatt aaatttaagt tttgattcta agtagatttt aattttaagt aatctatata
    12481 aaattaactc tgta
//
LOCUS       DAYAOX010000111        12435 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.111, whole genome shotgun sequence.
ACCESSION   DAYAOX010000111 DAYAOX010000000
VERSION     DAYAOX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.111"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..206)
                     /locus_tag="ACGI57_002340"
     CDS             complement(<1..206)
                     /locus_tag="ACGI57_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFG9717415.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQI"
     gene            complement(220..1122)
                     /locus_tag="ACGI57_002341"
     CDS             complement(220..1122)
                     /locus_tag="ACGI57_002341"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFG9717416.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(1317..2492)
                     /locus_tag="ACGI57_002342"
     CDS             complement(1317..2492)
                     /locus_tag="ACGI57_002342"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFG9717417.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(2579..3424)
                     /locus_tag="ACGI57_002343"
     CDS             complement(2579..3424)
                     /locus_tag="ACGI57_002343"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HFG9717418.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(3522..4319)
                     /locus_tag="ACGI57_002344"
     CDS             complement(3522..4319)
                     /locus_tag="ACGI57_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HFG9717419.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(4338..5348)
                     /locus_tag="ACGI57_002345"
     CDS             complement(4338..5348)
                     /locus_tag="ACGI57_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFG9717420.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(5367..6149)
                     /locus_tag="ACGI57_002346"
     CDS             complement(5367..6149)
                     /locus_tag="ACGI57_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFG9717421.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(6208..7344)
                     /locus_tag="ACGI57_002347"
     CDS             complement(6208..7344)
                     /locus_tag="ACGI57_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFG9717422.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(8053..8520)
                     /locus_tag="ACGI57_002348"
     CDS             complement(8053..8520)
                     /locus_tag="ACGI57_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFG9717423.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(8599..9141)
                     /locus_tag="ACGI57_002349"
     CDS             complement(8599..9141)
                     /locus_tag="ACGI57_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717424.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(9193..9879)
                     /locus_tag="ACGI57_002350"
     CDS             complement(9193..9879)
                     /locus_tag="ACGI57_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFG9717425.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(9879..10739)
                     /locus_tag="ACGI57_002351"
     CDS             complement(9879..10739)
                     /locus_tag="ACGI57_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717426.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            10874..11674
                     /locus_tag="ACGI57_002352"
     CDS             10874..11674
                     /locus_tag="ACGI57_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HFG9717427.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            11699..12268
                     /gene="yfcE"
                     /locus_tag="ACGI57_002353"
     CDS             11699..12268
                     /gene="yfcE"
                     /locus_tag="ACGI57_002353"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFG9717428.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         3946 a         2274 c         1376 g         4839 t
ORIGIN      
        1 attatttgtt cacaacagtt tttaattaaa ttgtcattat aatctttaaa aacacctttt
       61 cttaaatcct catcttttgc aattcttttt atagctttct ggaaaaaacc atccacttct
      121 tgattaactt tgccttcaaa gtcatttaat attggaacat cactattttt gtccaaaaca
      181 tgtattataa atttatgtat tatcatatat atcaccccat tatcttaatt tcgatattaa
      241 ctctttcata tcctctggta tatctgcttt aaatttcaat ttttcttttg ttcttggttg
      301 ttcaaactct agcttatatg catgaagtgc ttgcctattt attagctctt catctacata
      361 accatacaac tcatcaccta ttattccatg acctatatga gccatatgta ctcttatttg
      421 atgtgttcta ccagtttcta agcttacttc taatacagtt gcattttcta gtctttctat
      481 tactttatag tgagtaacag atgactgtcc tctttcatct attatcctct ttattgaatc
      541 ttctgtaggt ctataaattg gctcatctat agttccctca ttttctttta taattccatc
      601 aactactgtt atatactttt tttcaacttt attttcactc attgctgtag acaaagtatg
      661 atgtgcataa gcattttttg ctactataac taaccctgaa gtattcatat ctaacctatt
      721 tacaaatctt atttttacgt tttctttttg attatctata tagtaactaa taccatttgc
      781 tattgtttta tcatagtggc tttttgttgg gtgaactacc ataaacggag gtttattcac
      841 cataattata tcaaaatcat cataaattat ctgtaaattt aaatcttgag gttcaaaatt
      901 tgctttttcc tcgtctattt ttacttctat taaatcacca ctatacacct ttaaactcgg
      961 ttttttatat actccattta ctaaaacact cttttccctc ttcatttttg ataatgacct
     1021 aactgagaaa tttagcttgt ccaataaaac ttcttttaaa gtcatttctt cttcatttgt
     1081 atacgatata agattatatc tttgattctc ttttttaaac aaacttttac ctactttctt
     1141 ttatatcttc ctacttttta tatcttcaaa atcatgatat tatatatttt acactatttt
     1201 tgtttaatag ataataatta tttttaacta taaaaacctg taagaataac aaatagttat
     1261 tcttacaggt taaattcata attaaatatt taatttgtaa atattataat ctaaaactat
     1321 ctcttaacta ttaaagtagt tcccattcct ccacctatac aaagtgtagc aagaccagtt
     1381 ttagcatctc ttctcttcat ttcatataaa agtgtagtaa gtattcttgc tcctgagcat
     1441 cctattggat gtcctatagc tattgctcca ccattaacat taactttatt catatctata
     1501 tttaagtctc ttattacagc tacagattgg gcagcaaatg cctcattagc ttcaactaaa
     1561 tctatatctt caatagtcat attagcagct tctaaagctt ttttagttgc tggaactggt
     1621 ccatatccca ttattttagg gtcaacacca gctgttccat aagaaactat agttgcaaga
     1681 ggctctattc ctagttcttc agctttttct ttagccatta ctactagcat agcagcacca
     1741 tcatttattc ctgatgcatt accagcagta actgttccat cttttttaaa tgcaggtctt
     1801 aacttagcaa gtttctccat tgtagtgcca ggcttaatat attcatcttt atctactaca
     1861 gtgtcacctt ttcttccttt tataacaaca ggaactattt cttcatcaaa ttttccttca
     1921 gcttgagctt tttcagcttt attttgactt gcaagagcta attcatcttg ttcttctcta
     1981 gttatattcc attgctctgc tatgttttca gcagtaatac ccatgtgata gttattaaat
     2041 atgtctgata atccatcttt tatcattgaa tcaacaaaag cagcatcacc cattcttgca
     2101 ccatatctcg cacttggtac taaataagga gacatactca tgttttcagc tccaccaact
     2161 aacattatat cagcatcacc taatgctata agttgagatg ccattgaaac agatcttaat
     2221 ccagaaccac aaactatatt tatagtcata gctggttttt ctactggtat tcctgctcct
     2281 aatgctattt gtcttgctat attttgtcca agacctgctg taagtactcc ccctaaaaga
     2341 gattcatcta tcatatctgg agttatgtta gctcttttta tagcttcttt agctgctgtt
     2401 acccctaact ctaccgctga aactgattta aatgctcctc caaaacttcc tactgctgtt
     2461 ctagctgcac tggcaattac tacttctctc atattcatta cccccattaa attagatttt
     2521 tcatatatcc gaaattgttt tttaaacaat tttatttaaa cttttaattc ccaaattctt
     2581 atttattata atcatagaat cctatcttag tttttcttcc taattgatta gctctaacca
     2641 ttttagctaa aagtggatga ggtctatatt tagtatctcc aaattcagta tataaaacgt
     2701 tcattatagc taaaacaaca tctaatccga ttaaatcacc taatgctagt ggtcccattg
     2761 gatggtttgc tcctaatttc atagcttcat ctatttcttc tttacttgca acaccatctg
     2821 catatatacc aacagcttca tttatcatag gtataagtat tctatttact acaaatccag
     2881 gagattcaga tacatctact ggtactttat tgatactctt agataattca aatactgtat
     2941 caaaagtaac ttttgatgtt aactgaccac ttataacttc aactaatttc atcataggaa
     3001 ctggattaaa gaaatgcatt cctataactt tatctggtct cttagtagaa gaagctattt
     3061 ctgttataga taatgatgaa gtatttgttg ccaagatagt atcttcttta cataattcat
     3121 ctagtaactt gaaaacatct ttctttatat tcatgtcttc tacagatgct tctattatta
     3181 aatccatatc ttttaaatct tcatagttag tagttgaact aacatgactt aatatttctg
     3241 cttttgtagc ttcatccatt tttcccttag taactaactt agttaaattt ttatctaata
     3301 aagctaaaca tttatctata gcaccttgag ttctactctt taaacataca tcatgtccac
     3361 aacttgcaaa agtttgtaca ataccacttc ccatagttcc actacctatt acagctaatt
     3421 tcatagttat aacctcctgt tattttagta tttatattct gttaactaga ttaaaatact
     3481 atttaatcta gttaacagca aatatatttt taattagatt tttacccttt tataaagtta
     3541 gcttctctct tttcaacgaa agctgacatt ccttcttttt ggtctttagt tgaaaaacaa
     3601 agaccaaata aattagattc tatatctatt ccagtattta tatcagtttg agcaccaagt
     3661 tgtattgctt ctttagagta tctaactgca agctgagcat ttttagctat tatcttagct
     3721 aatttctcaa cttcttctat taaaatgtct ggctcaacga ctctatttac tagccctatt
     3781 ttttcagctt catcagcttt tataacttga cctgtaaaga ttaattcttt tgcttttgcc
     3841 attccaacca atcttgtaag tctttgagtt cctccatatc ctggagttat tccaagagtt
     3901 acttctggct gaccaaattt agctttagca gatgcaattc ttatatcaca tgccattgca
     3961 agttcacatc ctccacctaa agcaaatccg tttacagcag ctatcactac ttttttacta
     4021 ttttctattt ctccaaaagc ttttgctcct aagatactaa aatctttagc agctacagca
     4081 tctaaatctt tcatgtatgc aatatctgct ccagctacaa atgcctttcc ttcccctgtc
     4141 aatattacaa catcaatagt ttcatcatta ttaatatcta caaatacttc ataaagttct
     4201 tctaaagtct ttgaatttat tgcattaagg gctttaggtc tattcatttt cactgtacat
     4261 atatttccat ctacttcaac agctacattc tcataaactt taacatcact tgtactcata
     4321 ataccctccc caagaaatta gttagctaaa acttcacctt tttcttttgc aacacttaac
     4381 tgactgataa gttctggaag cactttatga acatctccaa ctataccaac atcagcatat
     4441 ttaaatattg gagcttctgg atttttattt atagcaacta taaactcagc atcttccata
     4501 ccagctatat gttgtattgc tccagatata ccacatgcta tataaaggtc tggtcttaca
     4561 gttttaccag tttgaccaac ttgtcttgct ttatctaacc aacctgcatc tatagtggca
     4621 cgagaaccag aaacttctcc acctataatt tcagctaatt cataaagtat gtctaagttt
     4681 tcttttccac ccattccacg tccagcagaa actaatatct tagcatcttc tattttaact
     4741 tgttttttag cttcttttat tacttgtaca acttgaacta atttatcagc atcattaaat
     4801 tctaccttga aacggttaat tacagcttct ttagtttcat caggttcatt tttcttcata
     4861 acccctggtc taactgtaga catttgaggt ctgaaatctt tacaaactat tgttgccatt
     4921 atatttccac caaaggcagg tcttgtcatt aataataatt ttgtatcttc agctactgca
     4981 agacctgtac agtcagcagt aagacctgta tgtattctag cagaaactct aggtgctaaa
     5041 tctctaccta ttgaagttgc accaaataat acaactatag ggtcagctgc ttttattgct
     5101 tcataagctg cttttgtata tggttcagtt gtatacactg ctaaagcttc atcatctact
     5161 actattacct catctgcacc atagtgtgct aatgtatcta ttaaaccttc taccttacta
     5221 cctaaaagta atgcagaaac ttttgtatca taatcttttg ctatttctgt agcctttcct
     5281 agtaattcta aagaaacagt ttgaattaca ttttctcttt gttctattac tactaaaacg
     5341 ttacccattc tttatccccc cttaaattat atgatatact tctcttttaa tttatctatg
     5401 ataattccag ctgatgtttt cgcatcttca ttgtatattg taccagctgg tttaactgat
     5461 tttgtaaatg atttaaatac actagttgga gaacctttaa gacctaaatt agaagggtca
     5521 acttctatat cttttacagt ccatgtttct actacatcat tttcgaaagc atcatatatt
     5581 cttccaactt tcatgtatct tggtgtgttc atatctttaa gagttgttat aaggcatggc
     5641 attttaactt ttaagtcatg gcaacaatct tcaaattgtc tttttactaa tacatattca
     5701 ccttcagttt ttatttcttc agcatatgtt attgatggaa gatttaaatg ttcagctatt
     5761 tgaggtccaa cttgtgcagt atctccatct atcgcctgtc ttccagctat tataatatca
     5821 aaatctatat tttttaatgc tcctgctaat gctgatgaag ttgcccaagt atcagcacct
     5881 gcaaatgctc tatctgttaa taatatacct ctatctgcac ccattgctaa agcttctttt
     5941 aaagccatat ctgcttgagg aggtcccatt gttataacag ttacatgagc acccatttct
     6001 tcttttaatt ttatagcttc ttctaaacct gctttatcat cagggtttat tatacttggt
     6061 actccatctc taattaaagt acctgtatta ggatctagtt taacttctgt tgtatctgga
     6121 acttgtttta tacaaacgac tatattcata aatccttcct ccatatctat tttaatatcg
     6181 acctattgtg tatattttaa tatttatcta ttttaataat tttcctgaaa taaccattct
     6241 ttgaacttca ctagttcctt catatatttc agttatctta gcatctctca tcattctttc
     6301 tactggatag tcacgagtgt atccatatcc tccatgaagt tgtacagctt ttgtagtaac
     6361 ttccatagct gtttcagctg caaataattt tgccattgct gcttctactc cataaggttt
     6421 tcctaagtct ttatttatag ctgcttgata tacaaggtgt ctagccgctt gtaccttaac
     6481 ttccatatca gctaattgga attgtgtatt ttggaatttt gataatggtc taccaaattg
     6541 tactctttct tttacatatt taacagtttc atcaagagca ccttgtgcta aacctaaagc
     6601 ttgtgcagct ataccaattc taccaccatc aagagtagac attgctatct taaatccttg
     6661 accttctttt ccaagtaaat tttctttagg tattctgcaa tcctcaaata ttaattcact
     6721 cgtagctgaa cctcttatac ccattttctt ttctttaact ccaaagctaa acccaggagt
     6781 tcctttttca actataaatg ctgatattcc tttgttcccc ttagatttat cagtcattgc
     6841 cattactaca tatatgtcac cagctattgc atttgttata aatatttttg agccattaag
     6901 tatgtattca tccccgtcta aaacagcagt tgtttgttgg ccagacgcat ctgtaccagc
     6961 attaggctca gtaagaccaa atgctcctaa tttttctcca cttgctaatg gtcttaagaa
     7021 tttttgtttt tgttcttcat taccatattg atatataggc catgagccaa gagatgtatg
     7081 agctgataat ataactcctg tagtaccaca aactctagac aattcttcaa ctgccattat
     7141 atatcctaca gtgtctccac cttctccacc atattctttt ggatatggta tacccatcat
     7201 tcctgctttt gccatttttt ccactgtttc ataaggaaat ctttcttctt catcaagttc
     7261 tgttgctaaa ggtttaactt cattttcagc gaagcttaca tatagctctt taagcatctg
     7321 atatttttta gaatttaaat ccataatagc ctcctcagta ttttataaat attttatttt
     7381 catttattaa ttaaaatctc ggcaacatct ataataaggt atttgaatat acgtcgagaa
     7441 atttataacc ttaatcaaac tacgctctat atttatatgt gattatacta accaattatg
     7501 acattaattt aatcataaat tgttaattta aaatcaacta tacttaaaaa aaatctcgct
     7561 ttttggaatt ttgtgaaatt tcaagttaga ataagatttc tttcacactg gttagagata
     7621 aaataatttg tttacgatat ttatattaag gtatattaaa cattaaatat tgtctgtaca
     7681 tctgtttttt agccatgtac agaagatttt tatcgtataa aaattatttt aatagagcta
     7741 aataatcata tttttatctg actatttttt atttttaaat atcttacatc tataatagta
     7801 tcactcaaag gataattttt caataatttc taattatttt tttaacaata tttattacat
     7861 tttttccact tttttgtcat ttttttattg tttcgaaaaa aaatcagaat tttttaactt
     7921 tattttggat tattagttta aattagattt tcctcttaga tttataaata tttacacaaa
     7981 aaaactacct ctgtatagat tgtatacaga ggtagttttt tactaattcc tactatagtt
     8041 tactatattt ttttaccaat tcttacaaac atctacccat ttttcacact tagaaaactt
     8101 ctccatttct tcacatgtaa gttctattgc agagttacta ctgccacatg caggaaatac
     8161 tgtatcaaat cttttcattg aatcatctaa atagactttt atagaatttt ttaaaccaaa
     8221 tggacagact ccaccaatag catgtccagt aaactccaag acttcttctg gtgtcaacat
     8281 tttagcctta caattaaatt cagccttata cttcttatta tcaatcttag catcaccagc
     8341 tgtcacaatt aaaattgcat catctcctat tttaaatgag agtgtcttag ctattcttgc
     8401 tggaataacc ccagctgctt cagctgcaag ttccacagta gcactagatt gttcaaattc
     8461 taatatactg tcttccttgc caaattgttt aaaatactct cttacattac ttattgacat
     8521 taaatacccc tcctagtctg aacttttata aatttcatat tattcttaat tatatctata
     8581 attgatttat ttttcaaatt attccataat attagcattc catgtatcat tattttttcc
     8641 aactactaga taaagtcctt ttgccattgg aatattgaca atatattctc ctttatactc
     8701 tacactcata ccagattcat taaagtacct ataatcaaaa tttagtcctt ttggaattac
     8761 aaaatcaaca taaccttgat tatttggttt tattccatat ttcagagttc tttcagttat
     8821 agaatttttt tcattaaata aatctacatt tacatatgca gaacacttat tcattccctt
     8881 tttattttcc acacaatttt caaacttcat tttagcactg tttaatttat aactatattt
     8941 actattattg cttattttaa atcttacaat agtaccaaca tctctacttc tcctgctatc
     9001 aaaatcaaaa acaatttcct ctaaatgatt tatttcataa tctttgacac ttaccatatc
     9061 tctatatact aatttattta tagctatgaa tagtgtaaaa atccccaaaa taaaaaatga
     9121 aacttttata aacttcttca tttttagtct ccttatattt tgtgctattg tgctataata
     9181 ttaatcaaaa ttttaataat taaacttatt cttttttgaa atcttaatag aaaaatacat
     9241 tgatattata aaaattatta ttgttaaaaa tgtcatacta tataaattta tatacttagt
     9301 attagctata tcagttacat aatttgaaag atatactcct ccaactttac tataattaaa
     9361 atgctttact tgatatacaa tcatactaat catcttcaaa agacttccat atacaaagta
     9421 aattaaaata cttactataa aaccacaaac tttcattttt ccatatccaa acttaaaaat
     9481 taaaggcagt aaaatcgtca aaattgctaa aaatatattt acagaaaaaa atacatcatt
     9541 taaaaccata tttctaaaga acactagtga tgtcaattta cttagtaagg acacaattat
     9601 tgttacagaa attattaatc ctacacctaa taaatatttt gagtaaacta tatcttctat
     9661 actaactggc atactcctta taaaaatcct tgaatcattt tcataatcac actcaaatgt
     9721 atttataaat atcaaataac taacaacaat acttactgta taatatgaaa ttgaattaag
     9781 gaaaaaataa aacacaaaaa atagcaatat gtactttaaa ttacggatac catctgaact
     9841 aatccctatt attaaatctt ttcttattaa attgaacatc atctcacctc atttacataa
     9901 taatacatta gttcttctat agtaggattt ttgaccttat gcttatcaat ttctatcaat
     9961 aggcttttta ctttcaagct atctccaact ttaacaagtg cctcattata atatttatac
    10021 tctttcacac caactatgct ttctttaatt gcttctaact ctgctttatt tgccctaatt
    10081 atcttatatt cttgttctat taattctttc ttaacagtaa aaatctgatt tcctctattt
    10141 ataaaaacaa gataatcagc aattttaatt aaattatcag tattatgagt agaaattatt
    10201 acactactac ttccattttt cacaaaatct tgtaagtact gcattatttc agttcttaca
    10261 attgggtcta tcccagcagt tggttcatcc aaaatcaata acttagcatt gtgactaagt
    10321 gcacttgata acataagctt ttcagattga cctttagata aattttcaat atgcatattt
    10381 tcttctatat caaacttatt aaggtattca tcaaaccttt ctctatcaaa attcttataa
    10441 aataaagaaa ctatttttct aaattcctta acctttaaat gcggataaaa ttgtaaagaa
    10501 tcatatacaa aaccaatatt gtttttaaag cttattggag aattttcaat agtttcatta
    10561 caaagttcaa tttcaccttc atctgcatca ataatcccca ttataatttt tattaaagtc
    10621 gtttttcccg aaccatttgg tcctataatc ccaacaatat ccccttcctt taactctaag
    10681 tttatattat ttaacttgaa ttttcctaat ttttttgtga tattccttat gctaaacaca
    10741 tcttcacctc ttatgcattt ttttataccc acctatacat attttaacac attcactaca
    10801 aaggtcattt ataaatttat aaattaagaa ttataaaact atgttataat acaaagtatg
    10861 aagaggtgaa tatatgaaaa gaattataac tataaataca aaccaattaa ataagtccct
    10921 tgagactaaa gaactcttaa ctagaaaact tatcaatgct ggctttgaag tgtattcaga
    10981 tatttatcca gatacagagc ttattatatc aattggtggt gatggctctt ttttaagaac
    11041 agttagagat ttcgattttc ctgaaattcc cataatggga ataaatacag gtcatctggg
    11101 gttttttcct gatattttac cagataagat tgatagcttt attgaagctt atacaaaaaa
    11161 agattatatt attcaagaaa tgtcacttct taatgcggaa gtatatacaa ctactagtgg
    11221 ttctaatatg cttgctgtaa atgaggttgt tataagagga gacaagtcta ggactataca
    11281 cctaaattta agccttgata ataaacatat acaaaatttt agtggagatg gtatgattat
    11341 ttctacttcc acaggttcaa cagcctataa ctattctgct ggaggtagta ttgttgatat
    11401 aaatcttgaa cttatgcaga ttacaccttt acatccgata aataccaatg catataggtg
    11461 ctttacatca agtataatat gttcaaatga atctgttata aagattgcac cagaatatag
    11521 atttgaagat tctgtattga ttgtcgttga tggagttgag cacagattta gacaaatcga
    11581 aaatataaaa gttagtatat ctgatgcaaa aataaaatta cttagaatgt caaactatga
    11641 gttttggcat agagtatctg aaaaattttt atagactgta cttaggagga tttttaatat
    11701 gaaaatagga ataatgagtg atactcatgg tagcttactt tactttgaaa aagcgttaaa
    11761 tgtattatct gattgtgatg ttttattaca tggtggagat gtcttatatc atggacctag
    11821 aaatgatatt ccagaaggat ataatcctaa aaaatttatt gagacattaa ataaattaga
    11881 aaacattgtg attgtaaaag ggaactgtga tgcagatgtt gaccagatgg ttatagaaca
    11941 tcctattcag agtccttatg tcatgagcca atttggtgaa attagaatta tattgaatca
    12001 tggttatata gaatcagaag aagaaattat tgacaaagca aagaaaatgg gtggtgacat
    12061 attagtttta ggacatactc atgtaaaaaa gctatacatg gatgataatt taatagtaat
    12121 aaatccagga agtacttcta tcccaaaaga tggaagccat tctgtcgcaa ttatagatat
    12181 cataaaaact gatgatgagg atgagcttga acttgatata aacttaattg acatcaatac
    12241 tggaaatata ataaatatca atgattagta aatttttaat aaaaataaaa taagctttaa
    12301 tttattaaac tcataataaa aacaagtaaa ccattttatt tctatctcaa ttagaagttt
    12361 aagtacaata ccttaaactt ctaattaaat agaatctggt ttacttgttt tattatatgc
    12421 tatattgaaa tataa
//
LOCUS       DAYAOX010000112        12237 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.112, whole genome shotgun sequence.
ACCESSION   DAYAOX010000112 DAYAOX010000000
VERSION     DAYAOX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.112"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..607)
                     /locus_tag="ACGI57_002354"
     CDS             complement(<1..607)
                     /locus_tag="ACGI57_002354"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFG9717429.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            complement(689..1390)
                     /locus_tag="ACGI57_002355"
     CDS             complement(689..1390)
                     /locus_tag="ACGI57_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFG9717430.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            1617..1883
                     /locus_tag="ACGI57_002356"
     CDS             1617..1883
                     /locus_tag="ACGI57_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFG9717431.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            complement(1986..2405)
                     /locus_tag="ACGI57_002357"
     CDS             complement(1986..2405)
                     /locus_tag="ACGI57_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HFG9717432.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(2672..3454)
                     /locus_tag="ACGI57_002358"
     CDS             complement(2672..3454)
                     /locus_tag="ACGI57_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFG9717433.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKCKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            complement(3519..4280)
                     /locus_tag="ACGI57_002359"
     CDS             complement(3519..4280)
                     /locus_tag="ACGI57_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFG9717434.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            complement(4785..4919)
                     /locus_tag="ACGI57_002360"
     CDS             complement(4785..4919)
                     /locus_tag="ACGI57_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717435.1"
                     /translation="MDLINTIDKVQQAHKPIDEIIETIRDIVDIIKILEPVYNFKSNQ
                     "
     gene            complement(5329..6159)
                     /locus_tag="ACGI57_002361"
     CDS             complement(5329..6159)
                     /locus_tag="ACGI57_002361"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9717436.1"
                     /translation="MLNKLCMYLERPELYKQSEINFWDDEYISEQLLKAHLDTNFEGA
                     SRNFNFIEDSVNWIVTVANPVNYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSIN
                     YAQNRNKETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSL
                     KPGGKLILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTL
                     VITEKDENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK
                     "
     gene            complement(6511..7413)
                     /locus_tag="ACGI57_002362"
     CDS             complement(6511..7413)
                     /locus_tag="ACGI57_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9717437.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSEGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(7463..7924)
                     /locus_tag="ACGI57_002363"
     CDS             complement(7463..7924)
                     /locus_tag="ACGI57_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HFG9717438.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(7976..8431)
                     /gene="def"
                     /locus_tag="ACGI57_002364"
     CDS             complement(7976..8431)
                     /gene="def"
                     /locus_tag="ACGI57_002364"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFG9717439.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(8403..8666)
                     /locus_tag="ACGI57_002365"
     CDS             complement(8403..8666)
                     /locus_tag="ACGI57_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9717440.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(8763..9479)
                     /locus_tag="ACGI57_002366"
     CDS             complement(8763..9479)
                     /locus_tag="ACGI57_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717441.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(9476..10159)
                     /locus_tag="ACGI57_002367"
     CDS             complement(9476..10159)
                     /locus_tag="ACGI57_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717442.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            complement(10156..11001)
                     /locus_tag="ACGI57_002368"
     CDS             complement(10156..11001)
                     /locus_tag="ACGI57_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717443.1"
                     /translation="MIKVDDLSFSYTERDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(11115..11705)
                     /locus_tag="ACGI57_002369"
     CDS             complement(11115..11705)
                     /locus_tag="ACGI57_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9717444.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            11994..12227
                     /locus_tag="ACGI57_002370"
     CDS             11994..12227
                     /locus_tag="ACGI57_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFG9717445.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT         4181 a         2003 c         1132 g         4921 t
ORIGIN      
        1 attcaaataa tttagctcct aaactcacaa acatttgtaa aatcaaaaat gcttttgtgt
       61 ctatcttatg attactttgt aaaatctctt ttgcatcttc ttgcgaaatt aaaagcgctt
      121 caatctcttc atctggttct aaaaattcat ctgttatatc accatcacat atacaataaa
      181 caaaggcaac agattcatca ctcattccag gagataaata aactttatca atacttttat
      241 tttttttaat ttctaaaaga gtaagtcctg tttcttctct aagttctctt tcaacggaaa
      301 tatctataga ctctccttca tcaacaagac ctgctggtaa ctcgtatata tatccattaa
      361 taggtactct aaattgtctt attaaaatta attttttact acttttatga agcccaacta
      421 ttacaactgc atctacacta tcttcttcat tcttcaaata aatatttttt aattgctcac
      481 tactttttct tgatgctacc atccaaacct tatcttcatc taatttgttt ttatactcta
      541 tttcaaaaag tcctataaac ttattttcaa tcattggatt tacattcttt attatagttt
      601 ttttcaaatt tactacgacc tttctttttt aatacctttt taacatcatt aagtaaatat
      661 aatatcttaa caagtattca acaacttatt atatttccat ttcaggatta tctttatcta
      721 caaaacctac tttaatttca caattaaata tttcctttaa caattttgct aattcttctc
      781 ttgcactcat taccttatct aaattagcat cagtaaaacc atctatagga aaaaatatat
      841 tttttgagct attagtaatt tttccttcct ctccttgtct ccatatccat cttctagttt
      901 ttaccatgtc tccaactgaa taaattaatt ctccttcttc cattaattca acatcagttt
      961 ctccaaatgg tacaaattta tccccttttt tagaaaatct aacatataca tcatcatttc
     1021 taaaatccat atcatgagca cccattggaa gtaaatattt taaagaaata gagtttccta
     1081 aatctacaat aggatttata tgaggaagtt tcttattctt agcaaccctt gttgtcattg
     1141 cttctatcga actcataaat ttatttgggt tcatgcctag acttctaaat gcctctctat
     1201 atggtataat ttcttctgat tcctttactt ttttatcttt gaagtaatct tctactctat
     1261 ctatacttat atctaaaagg ttgcttattc tttctatttc tttagtatta tctattcctt
     1321 ttgcaactac tacaccaaag catacatttt ctaatttatc aaaaacttct ttttctacta
     1381 taaatttcat tttgctttat cctcctgtta ttttacatta ttttctaaat agattttatc
     1441 actttgtgtc ttataaatat accccctatt tgctaattgt ttaatttttt taaattttcc
     1501 aattaatatt atatttgttc ttatcaaata ttttatatgt actaattaca aatttaactt
     1561 tccaaaacaa atatcttgtt ttataatgta taatatattt ttatggaggc aagactgtga
     1621 aaaattttat tctaatatat tttatagtga ttaattctat tgctttcttt tcaatgtaca
     1681 tagataagaa aagagcaatt agaaacgaat ggagaataaa agaagctact cttatgtcta
     1741 tagcagtaat tggaggtagt ataggttcta taattggaat gtattctttt agacataaaa
     1801 ctaaacacat taaatttaca tttggtatac catttatact atttttacaa tttttattag
     1861 tatattttta tattttaaag taattgaaat ataaaacctt attcttttca ataaattaag
     1921 gtgtataaaa ttaaaaattt tatacacctt tttctagcaa ttattaaaaa gatatttaat
     1981 taaaattaat gacccttatg tttagctttt ctcttttctt gtttcaaatt aaatagtctt
     2041 tctttttctt cttcttttct atttttagct aggttttttc tatcctgttt tctttcttca
     2101 tattgaagct taagagcatt ttgagcctta gtccctatag tttctttttc ttgttccttt
     2161 tttacttttc tctgcattct tttgtatcca atgttgtctt tggtcacatt tttttctaat
     2221 ttaatgctac caaaatctaa tgagaaaaat ctttcaagta taaattcata cacttctact
     2281 tctttaggtt ctgctccaaa aactacccta catgcttcat attttttacc atcttgtctt
     2341 tcaaatatac ctacccaaaa tggttcttca aataaaactg ttaattttcc actaattttg
     2401 tccattgcta gttcctcctt aaattacttt ctaaagaacg gacaaccaag gaggcaggtt
     2461 actgacagct attgctgcgt ctggactacc aaccagactg tgtttttatc tttatagatt
     2521 aaatattatc ttttttaatc atgcttgtca atcttagtat caacatctat catattgatt
     2581 taagtgaaat caagcttttt aactctactt tattattgtt aacactaaga aaattaatta
     2641 tttttcataa taaattagtt attatactta tttaaatatc aatctttatt gttccctgca
     2701 taaacaagac tgcttcacca gaaattttta cagtattatc cttcaacact tcacactgaa
     2761 ttataccacc acgattagaa agttgagcag ctaccatttt gtgttttccc aacttttcag
     2821 accagtatgg aataagatta caatgtgatg aaccagtaac agggtcttct gaatctaatt
     2881 ctggacaaaa atatcttgat acaaaatctg tattacttcc ttgtgctgtg attattatac
     2941 ctaaccagtc tgtcaattta cgcaattgtg cgtaattagg tttataattt ataacttctt
     3001 gttcactgtt tagcaataag attaaatctc ttgaagaata aacagcactt ggtacacagc
     3061 ctatcaaatt agcttgttgt ggtgataact ctatttcaat aggcattatt tctggaaata
     3121 tcatttcata tagatttcca tttcgtgtaa cttcaagctt tccactttga gtaaagaaat
     3181 ctatcctttt tacattaaca tcaataaaat ttgatattac ataagctgct gctaaagttg
     3241 catgaccaca taaatcaatt tctgctttag gagtaaacca tcgtaattca taatttcctt
     3301 tacatttaac tacaaatgct gtttctggta aattgttttc ttctgctatt ttctgcatta
     3361 actctaaagg tattcttctg tcaagaacac aaactccagc aggatttcct ttaaatagtt
     3421 tagttgcaaa tgaatcaact atgtagtatt ccattattta acctctcttt ctaacataaa
     3481 atatacatca atttacattc aataaaactt taaatttctt acatatattt tttaatatta
     3541 ctctgttcaa caactttttt acctaacaat gtaattgctt ttttggcaca attatttaca
     3601 caacgataac acattgtaca ttgcccattt gcaacaatca tacaattttt aattactaga
     3661 ttcttcattg gacataaatt aacgcattta ccacatacaa tacattcctc agtattaatt
     3721 tttagtttat ctgtatacat atttgtttta ttgataaaat acaatcgttg tccaaataac
     3781 cctacaatat gactcaaaaa acccaaccct tcttttggtg gtgacccatg tttcagtttt
     3841 ttagcagctt tagttacttt cttttctgct tttattacaa gctttctatt atactctaaa
     3901 gaatgtttta ataccttttc atctcctata ctatctggca tcttcacatg taatccacca
     3961 acagttatag caccgtattt ttttaatatt ctagctaaca caccagagcc atctccacta
     4021 aatagagcca tagttgcaat aataaaaaca cgtttaccat tccacaaagt tgaatgattt
     4081 attataaaat ctttcaatat ttttggcaca ttactatatt gcacaggata actaaaaata
     4141 atattctcat gattattgat gtgctgtaaa agttcattat cttcaataga ataagaattt
     4201 gctgttatat catattcttg taaaaatttt tctacacagt atcttgaatt tcctgttcca
     4261 ctaaaatata ttcctatcat agtacttctc ctaatagcta agattatata gctctattct
     4321 taaaattctg actttttata ttatctaaat taagtagttt atttaaataa tataaactta
     4381 attcaacaag ttacctactg aacctcccat gactaaagtc atagtgttcc tgcttgatag
     4441 aattttgcat agtcaattta ctaaatactt gtctaattct ttttttatca ttttaaatat
     4501 acattttaat aatattagta agctatactt ccttaagcat atattttccc taaagtaaaa
     4561 ctattgatga ttattccatt atataaccta attataaatt tagtaagttc atcacatact
     4621 atacccttaa ttctcaatta taaagttccc taaaaaatat ttgaaaattt aactataaaa
     4681 aaattcccta ttgcctaata tgtataagta aggagtacta tatagtactc cttatattgt
     4741 actcttataa cattgatgcc ttaatattat tttaatctta atcattattg atttgattta
     4801 aaattataaa caggttccaa tattttaatt atgtcaacta tatctcttat agtttcaata
     4861 atctcatcaa ttggtttatg tgcttgttgc actttatcaa tagtattaat taaatccatt
     4921 tacacatcta gcacctgtat ggcaatttag cattatcctt atcttatact ttgttgtaaa
     4981 ttcttgatta cataattcta ttatctaact tatagctgtg caatctacat tatctgtaaa
     5041 tatctttgct ttatatattt tccctgaacc tctaacatta atataacagc ctttttgcta
     5101 tacactttta actatatact taattttaat aacattactt gtttataaaa atatactata
     5161 gataaatttc acataaaaat aataattcaa gtattgattt ttatataaac aaatttgtaa
     5221 tgtactaaaa ataacataaa agtttccatt tttcaatatg taagcttttc ttatgttatt
     5281 ttattgttaa aggtttcaag tttttattta ttcattttaa aaaaattatt atttttgtaa
     5341 aactaatccc atagtcatac tatcgtcaga atatgcttca cccttaacat tactaaaaaa
     5401 ctcaacagac ttaaatccaa tattttcaac ttctaataag aaactttctt ttgaaaaata
     5461 gtgattccaa acataaaata cattttcatc tttttcggtt attacaagag tttgttctaa
     5521 tgtattataa tcattgtatt tacaattatc ttgtagacac atatattttt cattactcca
     5581 aaatcctcca tcttcattta tttcccaata tttaatttcc tcaaaattat tgtatttatt
     5641 tattgtaaat acatctaata ttaattttcc tccaggtttt aaactatcat aaattttttc
     5701 cattaataac cttctatttt cagttgacaa agctccataa tcacaataaa ttaaggtagc
     5761 caaatcaaat tcctcattat agttcatatt taaataactt tgaaacaagt aatttatatt
     5821 taaattagtc tccttatttc tattttgtgc atagttaata gaacgttttg agaaatcaat
     5881 tccagttacc ttataacctt tttgtgcaaa tttctctgca tataaacctg gaccacaacc
     5941 taaatcaagt aatttaggat aattaactgg atttgcaact gtaactatcc aatttacaga
     6001 atcctcgata aagttaaaat ttctacttgc tccttcaaaa ttagtgtcta agtgtgcttt
     6061 taataattgc tctgaaatat actcatcatc ccaaaaatta atttcacttt gtttataaag
     6121 ctctggtctt tctaaataca tacataattt gtttaacaaa acataccact ccttaaaatt
     6181 tatttttaaa ataaaaaaac cacagagttt actctgtggt ttaacttcaa aaagatgagt
     6241 ttaaataaga gtaaaataga accaaatatt tttaagcaca acaataaaac tatagcttta
     6301 ctttgtttgc ttattaaatt tgattttatt cactcttttc cagcaatact tatatcaacg
     6361 attttatgat atcaaatatc accgaacaac atcatccttt catattttta ttttatgaat
     6421 taattatata tttaaatttt atatttgtca ataaatttta attttaataa attttaatca
     6481 tcatagtaaa aaaaattttt ttatataaaa ctaatcaaaa aaagatatct gtacatctcc
     6541 tgagtttttt aacttttcct tcattatttt agagtaactg cttgatagag aatatttata
     6601 tctaagttca ttaaccattt tatacaattg atttttatac tccctattaa gtgaaccttc
     6661 actatatagt attgataatt tatcaaataa ttcaggaaac tcttttttaa caaagtcaaa
     6721 aaaaacacct ctagttatac cccttaagta taaagtccca ggtaaaacat aatgtacatt
     6781 acattctttt gcatgtctaa atagcgaatt tatattatca aaatcatctg taatatatgg
     6841 tataattggc ataatatgta gaccaactga tgcgttagtt tttctaaatt cttttaacat
     6901 tttaaatcgt ctcattgaat ccacaccatt tggctcaata agcctttgtg tatcttcatt
     6961 tactgtggta attgtagatg ctacattgat atatgtaatc ctagatagct tatcaattaa
     7021 atcgtaatct cttaaaatta aatctgactt tgtagaaata atagctggtg tcttgtattt
     7081 tattagcagg tttaatattt ctggcataat tttatagtca gcttctattg gttgataact
     7141 atctgtaact cctcctatat tgattacttc tttcttccat ttactgcttt taagttgtct
     7201 ctcaagtttt tctactatat ttgtttttac ataaatctct tcaaaataat tattggagtt
     7261 gatgtactta tgagagtaaa tagcataaca atatttacat ccatgctcac atccacgata
     7321 aatatttaaa tcccatttat atggcatact cctttttaat ttattacaag ctatttcaca
     7381 atttattgga atatatttta ttttattata caaataatca cctctttata tacaataata
     7441 ctataatgtt aatctatagt aattaaaaca tgatatttcc agaccacaat tcataatctt
     7501 tactatattt acaaagtaat atattccatt catttattgg tattcctcca catgttgctt
     7561 tttcttctaa attatataag cattgcttta aatacatttt attatgccaa tcatcatata
     7621 aattttctaa atttaaacta tgttcattag atatatcatt tgcaaaattt ctatttttcc
     7681 attttaatat acttgtatat ttaagttcat taggtttata atttcttttt aacatttcat
     7741 catgtacaat ttttgtataa aatttaaatt catctatact ataattcaat actttattca
     7801 ctaatagatg gtttggagta ccttttttat ctatagaacc ttttatagca agtagctctc
     7861 tccattgact tactaattga tttttaggta atacatcaat taaatcctta tgccataatc
     7921 tcataattta actccttact ataaaatatt ttataaattc aataaccaat ctatcttaca
     7981 attttacatg ttcaataatt ttatctgtaa aaactatacc atttaaatgg tctatctcat
     8041 gacaaagaca ttttgccata aaacctgaac ctttaatttc tattttctca ccgtattcgt
     8101 ttagagcttg cactgtaact ttttttggtc tttttaattt accccatact tcaggaaaac
     8161 ttaaacatcc ttcaacaaca atttgttccc cttcttgttt aataatttca ggattaacaa
     8221 gcttaataag tccttctcct aaatctataa caactaatcg ctttaatact cctacttggc
     8281 atgcagctaa tcctcctcca tttggagtat tgtacatggt ctctgccatg tcatttaata
     8341 tttctcttat cttattatct acgttttcta catacttact cttttttctt aaaatttcat
     8401 catcaaatgt tcttatcttt cttatagcca taatattctc ccctacttat ctgtgctctt
     8461 gattttcttt cactttcttc aaataaaaat acactttcaa ttggttcatt aaaaattctt
     8521 gaaatatcat atgccaacca tattgaaggt tcatttttac cagtttctat agcatttata
     8581 gcctgtctag atgttccaac taatctccct agttgttctt gtgtcaatcc taatttttct
     8641 cttaatatct ttattttatt tttcatattt gctccattca tgtaaggtta actttacatc
     8701 ttaaatgtaa catttttcaa cattactgtc aagtcaacct tacatttata tattaaattt
     8761 tcttatttaa ctattttttt actaaacttt ttaaataata ataaaatcca tattaatgat
     8821 ataacaattg aaataaacat atagacataa ttatcttcct taaatgcctt cgatatccaa
     8881 aaagttggta agaagaacag aatatattgt gctttattta aaataaaaaa tggtgctaaa
     8941 atacccatca taaaaacacc tgagaatttc gtaattacca ttccttctag tttatttgat
     9001 gataaagaaa tcactaatat tgatataata attccttgta ataaaactaa taaagaaatt
     9061 gataagttta acaagaaaga aattttagtt agagaaaaaa agataagtag aatcatagta
     9121 ataataaatg ctaagatacc tggtattcca aatcgtgaaa taagataacc agttttacca
     9181 agcggagtta ctattaaata tcttgaaatg ctgtcatcaa tttctcccaa tatgaccatt
     9241 gtagaagcaa aaaagaacat aaaaggtgta ataaaagcta aaagtaaatc aaacatcaag
     9301 aaatacggct ctaaatttaa ttgataataa aacttgttaa gtgatatatt ctgtattaat
     9361 ggtattccaa attttataaa tactccacat aatattggag aaacacatac tataaaaagc
     9421 atagcatctc tcttaatttg ttcaaactct tgtttaaacg catttataat ctttatcata
     9481 gttttactcc acctaccttt ttccaactgc tacaaataaa ataataagtt ataaaataaa
     9541 ttattattat aattgatatt aaaattaaaa tattaatcat tataaaattt ttatctccac
     9601 taataagaga aatacatata ttaaaagggt acaaatttgc atatgatttt gtgtcaagaa
     9661 gtacattgag tattggtgga ataaagcata taatctcaat aggaatactt aaaacaataa
     9721 actgattcaa actagatgct ttacttgcaa gtatcaatcc aagcaaagaa aatataatag
     9781 aagaaaaaaa ggtacctata gttgatatta taatattatc taaatttaat gtaatagcaa
     9841 taaacatagc tacaatacta gatattactc ctaatgaaat tacctttgat aaaatatact
     9901 cagatacttt tactggagat acagctaagg aatttaaaac cctttgactt ttctcaagaa
     9961 gtacaatagc tcccataaaa aacaatccca ttgctgctgg gtcagaataa atcataatta
    10021 tagctatttt ttctctcata aaagttggaa atacattgat aatacaaata tatataatac
    10081 ttaaaaaaag atataaaaaa taaaaaccat acttaaattg aaactttata tctcctaaaa
    10141 taagattttt tagcctcatt gtaaattcct ccctgttatc tcaataaaaa tatcatttaa
    10201 tgttggttca ctactatgta ttgataaaag tttatttttc tcaataagca tattcaaatt
    10261 tttatcatta gcagtattgt tcagaaaaca ttcagaagtt ttttctccat tatcaaagta
    10321 agtatatcta actttaatag ctccttttga cataataaga ttatgtggtg tatctaaagc
    10381 agatatttta ccatttacaa tgaacgctac tctgtcacaa agctctgttg catctaacat
    10441 gttatgagtt gttaaaataa tagttttacc tttagatttt tctgataaaa ttatatcttt
    10501 cataaccttg ctatttgatg ggtcaagacc acttgttggt tcatctaaaa ataaaatatc
    10561 tggattatgt aataaagctt ttataaaatt taatcgagat ttcatacctt ttgaatactc
    10621 agataccctt ttatttgctt cattttctag tcctactgat tttaataact catcaataga
    10681 tagtagtttt ttagaatata gagacccaaa atattttaaa ttttctattg cagtcaactt
    10741 ttcatataaa ctaggaaatt caaaatcaac tcctatattt tcataaaatt tatttgagtg
    10801 tcttttactc tctactccat ttacaatgac acttcctcca taattagtta tcattccaat
    10861 tagaatcttt tgtaatgtag attttcctgc acctgatggt cctaaaaatc ctagtatttc
    10921 cccttttcct actccaaagt taatattttg taaaaaatct ctctctgtat aactaaatga
    10981 taaatcatca actttaatca ttcaatatca ccctttcaaa tttttgacta ttttgactat
    11041 atggtcaata aaatataatt tttttgtttg ttaataattt ttaataaaag ttattagcaa
    11101 acaaaattta aattctattc cataagttgt ataactaatc cgtttatcat taatcttaaa
    11161 gcatcttcaa aacaattact tccaacctcg cgcttatata gcatggtaag aaaaactgct
    11221 ctaaatgctc cactaaagct ttctatgtct ttattttttg catttggtat gcaagatata
    11281 atctctccaa tttcaaagtc atcatgtcta aagtgttctt caattataga atctggtaac
    11341 tttctcacca atatctcaaa ttctccattt atcataagat ttaaaattga agtactatca
    11401 accatcctat agaatttcat aaatatatct gtcaattgtt cacatgtaat actttcatct
    11461 actctattta tctcatataa gatttctttt tgtatagagt catgtatatc tcttaatacc
    11521 tcaaaaaaga gcaattcttt tgattgaaaa aatagataaa aagttccttt tggtattttt
    11581 acccgtttta caagctcatc aacagtagtt tttctaatac catattgctc catacacaat
    11641 cttgcttctt ctttaagtct ttttattata tactctcttt ctttatcaga atacgattta
    11701 ggcatctttt ccctccagtt tgactaaaat aatttatata gtcaatataa tactttttct
    11761 atctaatgtc aatagtatta ttagtatatt taaaaataag cttaaaaact ttcgaaaatt
    11821 ctgaatttta tatagcattt tttattatct agtgttataa ttaagatata aataatatat
    11881 tattctaatt ttcatatcta tttgcttatt ttttaactta gtttctaata gaagtaatca
    11941 tatttatctt tttaaaagtt tcaactttaa taaaaaataa aggagttgat tttatggcta
    12001 ctactacaaa aaaccgtttt tacgattatg ttcaagaaaa cttaaataaa tatgttgaag
    12061 tacattctta ctttagcagt tgtcctctat atggtgaaat tatagaagca gatagcttat
    12121 caattacact ttgctcaaga tatatagatg aatctaatcc aactttaaat gaaacttaca
    12181 cattatattt accattaagt tcaataatat ctattagaac actataaaaa gttaatt
//
LOCUS       DAYAOX010000113        12032 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.113, whole genome shotgun sequence.
ACCESSION   DAYAOX010000113 DAYAOX010000000
VERSION     DAYAOX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.113"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            355..2433
                     /locus_tag="ACGI57_002371"
     CDS             355..2433
                     /locus_tag="ACGI57_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFG9717446.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            2433..3212
                     /locus_tag="ACGI57_002372"
     CDS             2433..3212
                     /locus_tag="ACGI57_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9717447.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            3205..4014
                     /locus_tag="ACGI57_002373"
     CDS             3205..4014
                     /locus_tag="ACGI57_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9717448.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            4034..4516
                     /locus_tag="ACGI57_002374"
     CDS             4034..4516
                     /locus_tag="ACGI57_002374"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9717449.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            4537..4941
                     /locus_tag="ACGI57_002375"
     CDS             4537..4941
                     /locus_tag="ACGI57_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9717450.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNANLLSDDE"
     gene            complement(5097..6257)
                     /locus_tag="ACGI57_002376"
     CDS             complement(5097..6257)
                     /locus_tag="ACGI57_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9717451.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            6465..8015
                     /gene="xylB"
                     /locus_tag="ACGI57_002377"
     CDS             6465..8015
                     /gene="xylB"
                     /locus_tag="ACGI57_002377"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFG9717452.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVSSLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            8111..9448
                     /gene="xylA"
                     /locus_tag="ACGI57_002378"
     CDS             8111..9448
                     /gene="xylA"
                     /locus_tag="ACGI57_002378"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFG9717453.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            9970..11169
                     /locus_tag="ACGI57_002379"
     CDS             9970..11169
                     /locus_tag="ACGI57_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFG9717454.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            11336..>12032
                     /locus_tag="ACGI57_002380"
     CDS             11336..>12032
                     /locus_tag="ACGI57_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9717455.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTML"
BASE COUNT         4670 a         1303 c         2166 g         3893 t
ORIGIN      
        1 gtatttataa ggtcactata catattttca agtccgatat tatctacata ataggcaaat
       61 gttattcctg tatgtgtttt aaaaaaacta gatgtatata ggtatcatca cagatattaa
      121 aagtataaat aaaagatatt aagaacaact ttccagcaat atattagaaa attttactaa
      181 aatccggaaa gcgattgaca aacaaagtca aataaagtat attattggta agttagttta
      241 atgtacaaat gaattatata taaaacctaa tttataaata acgatactaa acgacattat
      301 ttaacttaag ttacacaaag atagaatatt ataccattga tttaggaggt agccgtgata
      361 aaaatatctg aagactatat tgaaaaaaga tttgacaatg aactattgcg aatagaagca
      421 tggggaaaaa actctttacg tattaggtcg tttgtagacc aaaattttgt tgatgaaaac
      481 tatgctctta acgaaaaacc taagttaaat aaagatgata ttacaattaa taaaaatgaa
      541 gatggaagtg caatcattaa aaatggaaag ataaaagctg tcttagacca tagagataga
      601 attacttttt ataatgataa aaatgaaata ttattgaaag aatatattcg attaagagct
      661 gtaaaacatg atgatggtgg agaagatgta gggacaattg agattaccaa agactttaac
      721 tctactctaa agttaaaatc aagagaatat cgaccaagct atagtggaga atttgaagta
      781 actactagat ttgaatctga gccttctgaa aagatttttg gaatgggaca atatcaacat
      841 aaatttttag atttaaagaa tactgttttg gagttagccc aaagaaattc tcaaatatca
      901 gttccttttt atatttctag tctaggttat ggacttttat ggaataatcc gggaattgga
      961 aaagtatctt ttgctaaaaa tatgacagaa tggaagatgt tttctacaaa ttttatagat
     1021 tattggatta cttgtggaga aagcccaaaa gagttaaata aaaattactc tcaagttact
     1081 ggaactgttc ctatgatgcc tgaaaacctt ttaggactat ggcaaagtaa actaagatat
     1141 agaacttcag aagaagtttt agatgttgtg aaggaatatt ctaaaagagg aattaagctt
     1201 tcatcaattg caattgacta ttttcattgg cctaagcaag gtgaatataa atttgactta
     1261 gattattgga aaaatcctaa agaattagtt aaaaaattga aagaagagta ttcagtagaa
     1321 ccaatagtat ctgtatggcc tacagttcaa agtgatgcag aaaactacaa tgattattta
     1381 gaaaatggct atttagtcaa tgttaatcgt ggtgttagaa tgacaatgca aattcaagga
     1441 aatacagtct ttgtagatat gacaaatgaa aatgcaagag aatatgtttg ggatagaata
     1501 gataaaaatt ataaacaact tgggattgac tattattggt tagatgtagc tgaacctggc
     1561 tattctgtgt atgattttga taactataga tataaaaaag gaaatgtttt aagttgtggt
     1621 aatatttatc ctattgatta tttaaaaatg atttatgatg gtttacataa tgacatagag
     1681 tctgttgtaa ctcttgtaag aggagcttgg gcaggagctc aaaagtatgg agctttagta
     1741 tggtctggtg acattgattc tagttttgaa gcttttaata atcaagtaaa tacagggtta
     1801 aatatgggac tggcaggtat accatggtgg actactgata ttggaggttt ccatggagga
     1861 aatcctaaag acccagaatt tagagaactt atggtaagat ggttccaata tgctactttt
     1921 tcaccaattt tgagaatgca tggagataga ttgcctcata gtaaaccatt atcaaataaa
     1981 ggtggaggct caatggtaac tggagctcct aatgaaatat ggtcttatgg tgaagaagtt
     2041 gaagtaattc ttacaaaatt cataaaaatt agagagagct taaaaactta tttaaaaaaa
     2101 ctgatgaaag aagcgcatga agatggaaca ccagttatga gaactttatt ttatgaattt
     2161 cctgaagatg ataaaacttg ggaggtggat aatacatata tgcttggaga tgaaatttta
     2221 gtagctccaa taatgaatta taaagataga agtagaaaag tatatttacc aaaaggacat
     2281 acatgggaaa atatatttag tggagttagt tatgaaggtg ggaaaactta tgaagttgaa
     2341 tgcccactgg aagaaattcc tattttccta aagcaagatt ccagttataa ttttaaagaa
     2401 ctaaaaaata ttttgggaga ggtgaaatca taatggagcc acaattatgg caattattat
     2461 taatcgtact atatggattt tttataaact atgaaaaaaa ttcaacaatg tttggaacat
     2521 atcaaccagt aacagcaggt tttattactg gtcttatctt aggagatata aatacagggt
     2581 tatatattgg aggtacatta cagctattat cattaggtat tagtaacttt ggtggagcat
     2641 caatacctga ttatcaaaca gcaagtatag tagccacatt tataactatt acgactaagc
     2701 aagaagcatc tgtaggaatc tctataggta ttccagttgc tttattgatg gtacaattag
     2761 atgttcttag aaatactatt ggtatatggc tagttcataa agcagaagat ggagctaaaa
     2821 aaggaaatta taaaaatata acctatatgc aaatgttagg agtactatta acagcagcta
     2881 caacaggtat tccagttgct ctatctgtaa tatttggacc ttctttaatt aataccattt
     2941 tgaaatatac acctgaatgg ttaacaggtg gtttaacagt agctggagga ctacttccag
     3001 cagtagggat aggattatta cttagatatc ttcctgctaa ggagtatttt agttacttag
     3061 taattggttt tgttttagca gtatatatga aagtaccatt attaggtgta gctttaattg
     3121 gtggagcaat tgcacttata atttacaaaa agaatttgga aaatcaagaa caacaatata
     3181 ctgtagtagg aggtatggac gaagatgagt gataaggtat cattaaataa atcaaatgaa
     3241 aaggtgaaaa aaagtgtctt atggcgctgg ttttttacta gttctgtttc ttcaaattat
     3301 gagaagatgc aggccttggc atactgttat gcagtactac cttttttaaa agtagtatac
     3361 aaagataatc cagaaaagtt acaaagtgct gtattaaatc atttacaatt ttttaacact
     3421 aatccttggg ttgcccctta tattttgggt attaatgtag caatggagga gttatcaagt
     3481 gaagagactg aagaggctgt tgcttcaatt aaaacaggct tgatgggacc agtagcaggt
     3541 ttaggagata gtttatttgt cgttattccg tggacaattt ttggagctat agcagctaat
     3601 atggcgatag atggaagtcc atttggaatc ttactgtgga ttgtagtgag tgtagcattg
     3661 aaattattaa gtataccact atttaatgct ggttatacat caggtactaa gctaatatct
     3721 tcaattgaaa aaagtttaaa gattttaact gagagtacat ctattttagg tttaatggtt
     3781 gtaggtgcat taataccttc agttgtaaaa gctaatgttg ctttgaactt tcaacaaggt
     3841 gattttacaa tgaaaggtca agaaatatta gatcaaatta tgcctgggtt agttcctgca
     3901 attttagttg tgattgttta ttgggctctt aaaaagaata taaaacctat atacttaatt
     3961 ttaggagtaa tggttgtttc aataatttta tatgcattag gtattttaaa ataaggaaag
     4021 gatgatatta gatatggaaa tgaaaggtat taaaaatatt cgtatagatg accgtttaat
     4081 ccatggtcaa gttgcaacta tgtggagtaa taaattgggt gttacaagat taatggtagt
     4141 aaatgatgca gtagctaaca attctgttca aaaacaagtt ttaagaatgg ctacacctgc
     4201 aggtatagcg tcatctataa ttacagaaga gactgctata aaaaatatta ctgcaggaaa
     4261 gtatgaagga caaaatgtat tattaattgt taagtctcct gtggacctta ttccatttgt
     4321 agaggcagga tttaaaatac ataatataaa tgtaggaaat atgtcaagta gaaaagatac
     4381 aacagttcta agacctaata tcagtgttac tgaagaagag aaagaagcat ttaaaagact
     4441 tttagatgaa ggaattgaaa ttacaactat aatgacgcct gatgataaga aaacttatct
     4501 tgtagacatc ttatagattt aaagaggtgt attaaattga atagtattat tttgatgagt
     4561 catggcaata tggctgaaga agtcttaaat tctgcaaaaa tgataatagg ggattctata
     4621 gattatccta ctgtaaatat gaaacaagat gatggaatag aaggaacaat agaaaaatta
     4681 aaaaaagctt tagaagtgta caaggaaagt tcagaaataa ttataatggc agatttacta
     4741 ggtggaactc catgcaatgc tgctttatta gtagccaata atgacagtcg aattaaaatt
     4801 atttcagggc taaatttagg tatggttatt gaatcagctt ttttagatac aaaggaattg
     4861 gcagcaaaat tagagaaaat aggtaaagat aatatttcta tggttgaaca aaatgcaaat
     4921 ttattatctg atgatgagta ggaagattat attaatattt aaaacgaaat acttatgtta
     4981 atacttaatt gatatacaat ttttaaaaaa tatagctaat cttgcataaa ctatataata
     5041 aatctaattt tagaatgtat aatttaaagg gttatggctg tatatagtca taacccttat
     5101 atttcattaa ttaaagttaa attttgtata ttcaaaaagc tcttgatatt tacagcagag
     5161 ccaccatata gagatgcctc agaccctaat gatgaaattt ctatatttat tcctttgtta
     5221 aaagagctaa ctaacatatc ttttgtaatc tgtaatatct cagggatatc actaattatt
     5281 tggctattta aatatattat ttcaggagca taagttgtaa tagcattatt tatagctatt
     5341 gttaagtaac tacaaaactc atgaataact tttttggcat tatggttatc ttcataatat
     5401 agttgcttta ctatatcaga atctatttta ggaatatttt ctaaagaaga aagctgttca
     5461 aatacctttt tttctgaaca atactgttct aaacagccac gatttccaca aggacatagt
     5521 ttaccgttag gcatgatgat agtgtgacca atttctccac tcataccatt tctgccacta
     5581 tataatttat tatttattat aatgcctgaa ccaaatccac tgtgaatact gagactaagt
     5641 agtgagttat gtacagttga aaaagtattc tcagctaaag ctgttaagtt tgcttcattt
     5701 tctatatgaa taggaaagtc atactttttg ctaaggatgc tatataagtc aatctcatta
     5761 agattgtaat atggagtaaa caatacttta ttttcacaag taatcccatg tatagccaaa
     5821 gtaagaccaa ttaccttata aggagtgtct attttggata tgttataact atttataatt
     5881 tcatctatca attgtataac attgtcttta cttacttgta tatctgttaa tttcttagaa
     5941 tttataatag ttccatctaa gtaagataga gaagaaaata tatagtcgta tccaatgtcc
     6001 atacttaaag aaatccctgc acatttatta aatactaata atatgggttt tcttccgcca
     6061 ctatgagtac tatttccaat tcctatttca tgaactagag attcatcaat aagttttttg
     6121 gtaatagcgg atatagttgc tttattcaat cctatagtag aagctatact tgccctagaa
     6181 ataggaccat tttttataat ttgttcaagt accaatcttt cattcatttc tcgaatagtg
     6241 tatttatcag taaccaattt gatattcctc cttaaaataa tattgtaata ctttttacac
     6301 aaaaataaaa ggttattttg cattgacaaa gataattaaa tattttatta ttagttcata
     6361 agttagttta atatactaac aaaaataaag caagtaaaat atacctaaaa tataaaaaaa
     6421 ttaggatagg aaaacgatag ttatgaagtg gcattcaagg agggatgaag ttggagtatg
     6481 tattaggtgt ggatattggt acatcaggta cgaaaacagt tttatttgat aaactaggaa
     6541 atactataaa aagttgtaca tatgaatatc ctcttataca agagaaatca ggttgggcag
     6601 agcaggatgc aaatgattgg tggaaagctg tagtcgaatc tataagagaa gtagttcaaa
     6661 gttcaaatat aagcagtgaa tgtataaaag gaattggatt atcagggcag atgcatggtc
     6721 tagttatgct ggacaatgaa ggtaaaacat tgagaaactc tataatatgg tgtgaccaac
     6781 gaacagtaaa agagtgtgag gagataactg atttagtagg tgaagaaaga ctgattgaaa
     6841 taactgctaa tccagcactt acaggattta cagcatcaaa gatattatgg gttagaaaca
     6901 atgaaccaga tatatataaa aatactaata aaatacttct tccaaaagat tatataagat
     6961 acaaattgac aggtgaatat gctacagagg tatcagatgc aagtggtatg cagttgctcg
     7021 atataagaaa aagagattgg agcgatgaag tactagaaaa gttaaatata gataaaaatc
     7081 ttttaggtaa agtttatgaa tcacaagaga ttacaggtta tgtgactaga gatgtatcaa
     7141 gtttgacagg gcttaaagaa ggaacaatag tggtaggagg agctggagac caagcagcag
     7201 gagctatagg aaatggaata gttaaagatg gagtagtttc atctactata ggtacatcag
     7261 gtgtagtatt tgcttataca aaagagccaa aaattgataa agaaggtaga gttcatactt
     7321 tctgtcatgc aataccaaat acatggcatg ttatgggagt tactcaagga gctggcttgt
     7381 cattaaaatg gtttaaagat aatttctgtc aaagtgaagt ggaagtttca aattctttag
     7441 gtgaagatgt atatgagatt ataaataatc aagtaagtaa agtaccaaca ggatgtaatg
     7501 gattgcttta cttaccatat atgatgggag aaagaactcc tcatcttgac ccttatgcaa
     7561 gaggtgtgtt ctttggatta tctccaatcc attctaaaaa agaaattgca aggtcaataa
     7621 tggaaggtgt ttcatatagt cttaaagact gtatggatat aatagagaat cttaatatag
     7681 acgttaatga agtaagagcc tcaggtggag gtggaaaaag taaagtatgg agacagatgc
     7741 aagcagatat gtttaatcaa gatgtatata ctataaattc aagtgaagga ccagcacttg
     7801 gagttgcaat tcttgcatta gtaggagctg gaatatatga aaatatacaa aaagcttgtg
     7861 atgctataat aaaaacttca actaaattag aaccaataag tgaaaatgta gatacataca
     7921 atatatatca tagattgtat aaaagaatat acaaatcttt gaaagatgac tttaagttat
     7981 tagatgaagt agttaatgca aaaataaata attaaattag cttaaaaaga aaaataaaga
     8041 tagaaaatag ttttaaatta agaaaacata ctacatatat tatatgtgta aaatattagg
     8101 aggacatgaa atgagtgaaa tatttaaagg aataggacag ataaaatttg aaggagtaaa
     8161 atctgacaat gaattagcat ttagatacta caatcctgaa caagtggtag gtaataagac
     8221 tatgaaagaa cacttaagat ttgcaatgag ctattggcat actttatgtg gagagggtaa
     8281 tgacccattt ggagtaggca cagtagagag accttggaat aatataacag acccaattga
     8341 gatagcaaag attaaagttg atgcaggatt tgaatttatg tctaagatgg gaattgagta
     8401 tttctgtttt catgacagag atatagcacc agaaggaaga gatttagaag agacaaataa
     8461 gatattagat gaaatagttg agtatataaa agttaatatg gaaaagacag gtataaagtt
     8521 attatgggga actgccaata tgtttggaaa tccaagattt gttcatggtg catctacaac
     8581 atgtaatgca gatgtatatg catatgcagc agctcaggtt aaaaaagcta tggaaataac
     8641 taagtactta ggaggagaaa attttgtatt ctggggtgga agagaaggtt atgaaacttt
     8701 acttaacact aatacagaac ttgaaatgga taactttgca agattcttac aaatggctgt
     8761 agactatgct aaggaaatag gattcactgg acaattctta atagagccta agccaaaaga
     8821 gcctactaaa catcaatacg attttgatac tgctactgtt ttaggatttt taagaaagta
     8881 taatctggat aaatacttca aaatgaatat agaagcaaac catgcaacac ttgcaggaca
     8941 tactttccaa catgaattaa atatagcaag aataaacaat gttttaggta gtatagatgc
     9001 aaatcaagga gacctattat taggttggga tacagaccaa tttccaacaa atatatatga
     9061 tgcaacactt gctatgtatg aagtgttaaa gcaaggtgga atagcaccag gagggtttaa
     9121 ttttgactct aaagtaagaa gagcatcttt tgaggtagaa gacttattcc tagcttatat
     9181 tgcaggaatg gatacatttg caaagggatt attaatagct cataaattat tagaagatga
     9241 ggtatttgaa aactttacta aagaaagata tgcaagtttt agtgaaggta taggtaagga
     9301 tatagtagaa ggtaaggttg gatttaaaga gttagaatct tatgcactgc aaatgccagt
     9361 tataaagaat aagtctggaa gacaagaaat gctagaagct atacttaaca gatatatata
     9421 tgaagtagat acaatttcta ataagtaatt gtgtagtaat taaacttgga tgtttgaata
     9481 aaataacagg atatgttctt gtttttattc taaaacggac aaagataagt atttgtaaaa
     9541 attctcataa tcatgataaa ttaaggatat agacttaagt ataattagtt tatatcctta
     9601 aaatttctat aaaaaataat catttctata gttatcaata tttttaatta tcaatatctt
     9661 tctcaactaa taaataaaaa tcagtaaagt atctaaattt aagtcattat aatggataaa
     9721 ttttttagca tatctttttt agtatatcac tgataagaat ttactttcag gatatagcag
     9781 taaaatagat atttgaatat aattgttatt ttataacttt catgaaataa ttttgaaaat
     9841 acaaataaaa atttttaaag agaataatag tggaatatat cttcaagaat ttcttttaac
     9901 tgtttaggtg ataataaaat aatgatacaa tgtattaaaa tataaaatct gaaagtggat
     9961 gagaattaca tgaaaaagaa agtattattt ttagtaatta ttttagcatt tataatatta
    10021 ttaggtaatt ctacttattc tcaaaagaat cagttgtctg aagaggaaaa ggtggatgac
    10081 tttaattatg tgtataatgt tgtaaagaca ggatatccat atctaggtgt aaataagagg
    10141 cttaataata ttgattggtt agcaaataag gatgaatata caaaacgaat aaaaaacact
    10201 gaaaatgatg aagaatttat agaagaactg tcatctatat tatctgattt aaataataaa
    10261 cacacagaag taatagataa taagaaaaga tatgaacttt ttaagaaatc atactctaat
    10321 aatgattggt atgattttct taatgataaa aaagttgtgg atagatatga ctctatgaat
    10381 cttaaaataa aaatgccaga tgatattttt gtaaaaaaag aattaatttt aaaggatgtt
    10441 attaagggtc agattggata tatgtattta ccatcaatgt catcaaagaa tggctctata
    10501 aataatgatt taaaaataat tggagattat attaacactt tagaaaaaca taaagcttta
    10561 attattgata taagaggtaa tttaggtgga agtgataggt attggcaggg aatcgtatct
    10621 aaactcataa aaaatgatgt caaaataaat ggttatagaa tttatagaaa taataatgaa
    10681 atagtaaaaa aatatacaaa tgcaagaaat ataaaattaa atcctgtaga aaatttacca
    10741 gcaagtgtaa aggaaaatgc acctaaagaa attataaaag attttaatga ttttgaagat
    10801 acttcctata ctataaaatc tacagatgat tcaaaattta agggtaatat atacttactt
    10861 gttgatagaa aaatatattc atcatcagag tcatttgcaa tgttttgtaa agaaaataaa
    10921 tttgcgactt tagttggtga aactactggc ggagatggag ggtgtataga ccctgtattg
    10981 tttaatttaa aaaatagtgg tctaatagtt agaatggcaa gttgtatgta tttaaataag
    11041 aatggaatat gtgatgagga atttaaaact attccagaat ttaaagtaaa agattgtaaa
    11101 aggactgctg attttaaaga tgataattgt ataagaaaag cactagaact tgaaaatatt
    11161 aattattaga taaaagaata aaattaaaac tacatttttt taaacatcaa ttatagcagt
    11221 attttttata gtcaatttta tgggctaaat agtaaatata agaatacgct gtaatttaat
    11281 gttgaatatt ggtataatat ttatgacgat ttttaattaa agggggtaat agtttatgag
    11341 attagaagaa atgatttttt taaattataa taaattgaat gatacagatt tattaatatg
    11401 gaaatatata actgcaaata aaaaacattg tagtaagatg tcaataaatg aacttgctat
    11461 gaaatgtaat gtgtctcgtg caactattac aagatttgtt aaaaaattag atttaaaagg
    11521 tttcagtgag tttaaagtat tattaagctg ggaatcagaa aaggttcatg tcatagaaaa
    11581 tcagtcattt gatattgcat gtaattcaat aattaagtat gttgaagatc aaaaaaataa
    11641 agattatgat gcattgtgta aacttcttta tgaatcaaga acaatatacg cttatggttc
    11701 aggtgatatt caaaatgtgg ttgcaaaaca gataaagaga atgttcttat catgtcaaga
    11761 aataatatat gattttgagg gaagaacatt tgatgattct ttttacaata ttgtcagtaa
    11821 aaatgatgtt atgattatta tatccctaag tggtgagaat gagaatacac ttaatattgc
    11881 tagaagatta aaattaatag gaactaaaat aatttcaatt acagagttta aaaataatac
    11941 attgactgat ttaagtgatg aatcaattta tatttcagcg actaatatct catttttaca
    12001 aatgcaccct agttataaat tgacaatgtt at
//
LOCUS       DAYAOX010000114        11846 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.114, whole genome shotgun sequence.
ACCESSION   DAYAOX010000114 DAYAOX010000000
VERSION     DAYAOX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.114"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            204..1244
                     /locus_tag="ACGI57_002381"
     CDS             204..1244
                     /locus_tag="ACGI57_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9717456.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1600..2922
                     /locus_tag="ACGI57_002382"
     CDS             1600..2922
                     /locus_tag="ACGI57_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFG9717457.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3219..4580
                     /locus_tag="ACGI57_002383"
     CDS             3219..4580
                     /locus_tag="ACGI57_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFG9717458.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4653..6047
                     /locus_tag="ACGI57_002384"
     CDS             4653..6047
                     /locus_tag="ACGI57_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9717459.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6109..6579
                     /locus_tag="ACGI57_002385"
     CDS             6109..6579
                     /locus_tag="ACGI57_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HFG9717460.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6582..8798
                     /locus_tag="ACGI57_002386"
     CDS             6582..8798
                     /locus_tag="ACGI57_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFG9717461.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8881..9552
                     /locus_tag="ACGI57_002387"
     CDS             8881..9552
                     /locus_tag="ACGI57_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9717462.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9915..11129
                     /gene="dpaL"
                     /locus_tag="ACGI57_002388"
     CDS             9915..11129
                     /gene="dpaL"
                     /locus_tag="ACGI57_002388"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFG9717463.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11256..>11846
                     /locus_tag="ACGI57_002389"
     CDS             11256..>11846
                     /locus_tag="ACGI57_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9717464.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         4478 a         1357 c         2403 g         3608 t
ORIGIN      
        1 attgtaattg cttatttaat agaggatttt tgtaaaatga agaaagtata tctatcaaat
       61 taaaactaag tagatatact ttttattaag ttttgtttta aaataatatt attaaattat
      121 aactaagctt ttctgttatg atataattaa atagataatg tgaatgatta ttatatagaa
      181 tacttatttg gagttgatat tttatggaca aacacagtga ttcagtaatg tatggtggaa
      241 tggaagtata taatatttgg ggaacaacaa cagtagactc cataattgga gagtctgatg
      301 taatgcgtgc tcttaagaat agaatttgta aaattggaaa tagtgattct actgtagcta
      361 taactggaga aagtggcact ggaaaagaat taatagcaag agccattcat ctcgcaggaa
      421 acagagggaa taaaacattc gttgctataa actgtgctgc tataccagaa cctttactag
      481 aaagtgaact ttttggatat acaaaaggag cttttagtgg agcagattca aaaggtaaaa
      541 ttggtaagtt tgaattggcc aatggagggg ttgtattttt agatgagata ggagatatgc
      601 caattcaact acaatcaaag ttattgaggg tattacagga aaagaagttt acgagaatag
      661 gttcaaatga actaattgat atagatgtta gaatcatatc agctactaat agagatttat
      721 ttgagttggt taaggaaaat aaatttagag aagacttata ctatagattg aatgtaatac
      781 ctttagaagt gcctccactt agagaaagag gagacgatat acaaatatta ataaacaatt
      841 ttatagacaa atattcgaga aagatgaaga aaaacgtgta ccatatagag ccagatgtgg
      901 aacaaatgtt gcttaagtat ccttggccag gaaatattag agagttggaa aatactatag
      961 aactggcatt aaaccttctt gaggaagatg gaataataca taaaggaata ataaatagaa
     1021 agatacttga atattttaag ttaaataata taaatataaa gttacttgag gaaagtgact
     1081 atgagataag tatggaacaa gacatactta cacttgaaga gtttgaaaga gcctatataa
     1141 agaaagtgtt aaagttttac ggaaatgata ctaagtctaa aaaactagct gccaaaaaac
     1201 ttggaatatc actagctaca ttgtacagaa aaatagatgt ataataatta ggctttttat
     1261 tctcaattct aaaatttgaa attataaaat tgataaaaaa cacttggaaa actattttct
     1321 aagtgttttt ttgtgtaata gcattttatt atccaaaaat aaaaaaaatt taaaagaatt
     1381 taaaaaaata aatgctttaa taaattatca aatactaaca aatataataa aaattatata
     1441 tgtaaaacaa ttatgttaaa aaattggcat tataattgct tcatattaag ttgagatgac
     1501 aaaataaaaa aagaaaggat gttgtgtgag ataataataa ctgtcattaa aaaatagttg
     1561 ggagtgataa ccttgagtaa atatcaaaag aatgtttcta tgtttgatat tgatggaagg
     1621 ccaccaattt taaagtgttt gccactatca atgcaacata tactagcaat gatagttgga
     1681 actgttacag tacctatagt cgtaggagga gctgtgggtg ttagccctac acaaataaca
     1741 cttttagtgc aatatgcatt aataatagct ggtatatcaa cacttataca actatatcca
     1801 ataggaaata ttggttcaag attgcctata atttttggag ttggttttac ttatgtacca
     1861 acacttgtag cagtcggagg aaattatgga ttagcaagca ttttgggtgc acaattaata
     1921 gggggagtgg ctacagtatt aatagggata ttcataaaaa agataagaaa attttttccg
     1981 aatatagttg caggaacagt ggtttttaca attggattat ctttgtatcc tatagcaata
     2041 aattatatgg caggtggtgt aaatagtcct acttatgcct ctatgcaaaa ctgggcagta
     2101 gcggctataa cattagtggt agttataggt cttagtcaat ttgcaaaagg atatgcaaaa
     2161 ttggcagcaa tatttttggg aattatagtt ggatattgtg tatctttagt tctgggtata
     2221 ataaattttg accctattag agaggcaact tggtttgcga tacctaaacc gcttgaattt
     2281 gggatggaat ttgatatgcc tgtaataatc tcagtagtaa ttgtaagtat aatcaatgct
     2341 gttcaagcaa ttggagattt atcagctact acaatgggag gcatggatag gaatatcaca
     2401 gataaggaat tatcaagtgg agtaatagga agtggaattt gtactatgat aggagctttg
     2461 tttggaggtt taccaccatc atcatatagc caaaacgtgg ggatggtagc tatgaacaaa
     2521 gtaataagta gatttgtgct tggaatagct ggtatattta tgttattgtc ggggtttgtg
     2581 cctaaatttg gtgcactaat gactactata ccatattctg ttttaggagg agctacaata
     2641 tctatattta gtatgataac aatgacaggt ataaagttaa ttattacaga agagttgtca
     2701 tcaagaaata ttaccattgt tggtttggca attgccttag gcatgggaat aacatcagta
     2761 ccagcagccc aagaggcatt tccttcattt gtaaagttag tatttggaga atctccaata
     2821 gtaattgcaa cattggttgc attcgtttta aatctaataa taccaaataa aagtctagag
     2881 gatgaggcta aagaaagagc tgttatggaa gagactaatt aagatataat aatttaaaag
     2941 aattgtttaa attatttagt atgagattgt tagattattt ttgatagcta ttcattttaa
     3001 taaaaaattt aacaaataaa tatataccag gcaattaatg ttcatttaag ttgaaaatcg
     3061 gggagggaat atttagaaat ttgttaattt tttataaaaa tagctcagtt tagctgtgtt
     3121 tatcggttga gcaataaatt catggtcata aaaatattta tagaaaataa ttataaaaat
     3181 atttagtcaa gatatttatg tatgggagga tttattttat gaaaacacgt aatacttcaa
     3241 aatatgatgt cgatgggata ccaccactaa aggaatcaat acctttggcc ttacaacatc
     3301 tgttagcaat gatagtagga aatatggtac ctgcgatatt aatagcaaac gtggtaggac
     3361 ttaaccaggg gcaagcaaca atgttgattc agggttctat gttagcagca ggtttagcaa
     3421 cgtttttaca gttatatcca atacctttat ttaaaggatt taagttaggt tcaagattac
     3481 ctgtaatgat gggaatgagt tatgtatttt taggagcttg tctttcagta gcagctaaag
     3541 atggtttagc agctctattt ggagcacaga ttgctgctgg ggtaatcgta ttctttgtag
     3601 gatttggagt taagaaaatt agacatatat tcactccaat agtttctgga actataatag
     3661 catgtatggg acttggacta ttcgcaacag ctataaagaa tttagctgga ggagaaggca
     3721 cacaaacatt tggaagccca ataaacttca cagtaggagt tatagttgct tttgtaataa
     3781 taatgataaa taaatacggt aaaggtttag taaaaaattc ttcaatatta ataggaatat
     3841 tagtaggtta tgctatatct ttagttttag gattggttga tttctcagcc gttcaaggag
     3901 cagctatagt atctttacca acaccagcag cttttggatt agaatttaga ccagagttaa
     3961 tagtaatgtt tactattatt tacataatag gtatagctga tatgatgggt gcctgtacaa
     4021 tagctacagt aggagcaatg gatagagagg taactgacga agaattagca tcagttgttt
     4081 taggaaattc tataacttct ataatttcat cactatttgc agctcttcca actggagtat
     4141 ttagccaaaa tacagtaatt gtatctatga acaaagttac aagtagattt gtaatagcac
     4201 taggggcact tgtattatta ctagcaggga tatctccagc attgggtgca ataatgacaa
     4261 ctataccatc ttgtgttgtt ggtggagcaa ctttagtagt tttctcatca atagcaatgt
     4321 ctggtttctc tattatgagt atggatggat tcactgaaga aaacaatctt atagcaggtg
     4381 tatctatagc aacaagtatg ggtcttacaa ctgctccaca agttttagac caattcccag
     4441 aaacaattgg aactgtcctt ggagggtcaa gtatagtttc tggagcaata atagctatat
     4501 tacttcaaac attatttaaa ttaaagtcac gtaaaagtgc tgaaaacgtt acttcaaatc
     4561 ttgaggaaaa tattggataa atgtattaga tataatatga gaaaaccata tggtaaaatg
     4621 ttgtaatgaa agtattcaaa gggggattta agatgattga tatactaata aaaaatgcaa
     4681 taattgttac agttaataaa gacagagatg ttatatttga tggagcaata gctattcaag
     4741 gtgacagaat cttggacata ggaaatacag ttgatattga gcctaagtat cttgatgcta
     4801 aaaaagttat agatgctaat ggaaaagtaa tattccctgg ctttataaat actcataatc
     4861 acctgttcca agtgttatta aaaggtttag gtgatgatat ggcacttcat gagtggttaa
     4921 atacaatgat gtttccatca gcaaaattct taacagaaca agatacttat gatgctgcaa
     4981 tgcttggatg tatggaaggt ttaaaaagtg gtataactac aatggttgat tatatgcata
     5041 ctcacaatag accaggactt acagatggaa tagtaaaggc atataaagat ttaggtataa
     5101 gaggggttgt aggtagaggt tgtatagatt taggtattca caaagagctt atagaagatg
     5161 tggaaacggt tgaaaaagat ttgagaaggg tattcgaaaa atatcataat tcagaaaatg
     5221 gaagaataaa aatctgtgta gctccttctt caatgtgggc tatatctgaa gaaatgggta
     5281 agatgctttg gaatatagtt aaagaatatg attcatattt tacagttcat atatctgaaa
     5341 cagaatttgc aagaacagca acaaaggata tacatggaga aatagatata aaattacttg
     5401 aaaaatgggg tatagtaggt ccagaagttg tagcagtaca ttgtgtatgt ataactgatg
     5461 aagacatgga aatgcttaaa aagtatgaca taaaagtttc tcataatgta gcaagtaaca
     5521 tgtatcttgc ttctggtgtt gcacctgtgc cagaaatgtt aaaaaaaggt ataaatgtga
     5581 gtttaggtct tgacggtgca gcaagtaata acgcacaaga tatggtagag cttatgaaat
     5641 taacagcttt acaacacaaa gtaaataatt gtgaccctct ggcaatttct gcagagaaag
     5701 ttcttgaaat ggctacaatt gagggagcaa gaactcttag aatggaagat gaaataggtt
     5761 ctttggagat tgggaaaaaa gctgacctag taatatttaa ctcaatgctt tctccaaaat
     5821 caatacctct tcacaatcct gtatcaactt tggtatactc agctagtatg cacaatgtag
     5881 agagtgtttt agttgatgga aatgtaatac ttgaaaatgg aaaagtaact acaattgaaa
     5941 gtgaagaaca agtgttgctt aatgcacaac atgcagcaga agaattatgt aagagagcta
     6001 atgtaactaa tagaatggaa ggacataagt ggaatagctt atactaatat taaaataaga
     6061 ttgtaataat attctattac aacattagtt tatctgtagg aggagaaaat ggaaaaaata
     6121 aaattaaata ttaatgatta catgtatgaa atagaagttg aaaagaatga aactcttttg
     6181 catgtacttc gtgaaaaatt aaatctaact ggagcgaaac aaggttgtaa tagtggcagt
     6241 tgtggagcat gtaaagtttt gatagatgga gttgacatga aatcttgtaa acttcttgca
     6301 aaaaactgcg aaggtaaagt tatatacact atagaaagtt ttgctaatgg agaaaatctt
     6361 catccaatac aagaagcttt tatagaagtc ggagctgtac aatgtggata ttgtactcct
     6421 ggtatgatta taacaacaca agctctttta aatagaaatg catctcctaa tgaagatgaa
     6481 attagagaag cttttaaaga aaatctctgt agatgcacag gatatgtaaa gattatagaa
     6541 gctgtaaaat tatctgcagc tagattaggg ggtagataat tatggaaaag gtaataggaa
     6601 aatcataccc tctaaaagat gcagctttaa aagttactgg tcaaatgaaa tacgtggctg
     6661 atatgaaacc acaaaatgta ctttacgcta agatgctttt aagccctgta gcacatgcaa
     6721 aaataaaaag tatagataca agtgaagctg agaaagtagt aggtgttaga gctatagcta
     6781 cttatctcaa tacatcacaa agaaaataca atggttcatt aagattttat gagcacaaaa
     6841 tacctgaaaa tgaagttata ttttcagata cagttagatt tgttggtgac cgtgttgcag
     6901 cagtagctgc agagactcca gaaatagcag agaaagctgt aaaacttata aaagttgaat
     6961 atgaagaatt gccagctgta tttactatac aagatgcaat aaaagaagat gctatctgca
     7021 tacatggaga ttcaaatata gtttctcaaa acaacataaa tgggggagat gtagaaaaag
     7081 gcttcaagga atgtgattat atatttgaag atgaatactc tactccagca atacatcata
     7141 gtgcaataga gactcatagt gtaattgcac actatgatta taatgataaa ttaactgtac
     7201 atacaccttg ccaaaataca tttggattta gaataatact atctcaaatt tttgggatgt
     7261 cattaaataa agtaagagtt ataagacctg ctattggagg ctcatttggt ggaaaattag
     7321 aacttacaat tgaaccagta gcagccgcat tatcaaaaat gacaagaaga ccagttaaaa
     7381 tggttcttac aagaaaggaa tctatgatat caacaagaac tcgtcatggg tcttttgtaa
     7441 aattaaaaac tggtgtaaat aaagatggtg aagtaatagc acaagacata aaaatatata
     7501 caaatacagg agcatatgca tcaagtgctt taaatgttat aggagcactt agtcataaag
     7561 tatttaaagt ttataaaata ccaaatataa agtttactgg gatgcctgta tatacaaata
     7621 caccaatagc aggagctatg agaggctatg ggtcacctca aatatttatg gctcaacaag
     7681 ctcaatttgc gaaaatagca aaggaaatag gtatggattt agtcgatttt caaaataaaa
     7741 atgcagtaga gccagatgat gtcgatataa tcttccacgg ttctcttgga aatcctagag
     7801 tattggattg tatagagcaa ggtaagaaga tgtttaagtg ggatgaaaag aaaaaacttc
     7861 caaaagaaga aggaaaatat ttaagaggta taggtatggc aataggtgca catggtaatg
     7921 gagtttttgg tgcacataga gatgttgtta cattgactct taaattaaat gaagatggta
     7981 cgttgacact actaacagga gctcatgata tgggtaatgg tgttgtaact atgcaaacaa
     8041 tgatggtagc tgaagtttta ggaataacac ctgataaagt tgatacattt gaaactgata
     8101 cggatgcatg tacattcaac cttggtgact atgcaagtag aggggtattt gtagaaggtg
     8161 gaggagctaa aaagacagca gagaaattga aaaatcttat attagaagaa gcagaaaaat
     8221 tacttgaaac atcaaaagaa gatttatatc tagacaatgg atgtgtaatt agtaaacttg
     8281 atgaaaatgt aaaagcatca ctttctgatg tcgcagtata tagtcaaagt aaatcattaa
     8341 gagagttaac tgttgttgaa gattattctt caccagctgg acgtacttct tatggtgtac
     8401 attttgctga agttttagta gataaagaaa ctaaggatat aaaagtagtt gattttgtag
     8461 ctgtacatga tgttggtaga gttataaatc ctctaaattt agaaggacaa ttagaaggtg
     8521 ggattcatat gggtctagga tacgcattga gtgaagaatt ggcatttgat gaacaaggta
     8581 gacctaaagc tactgatttt aaaacatata aattatttag aactgtagat atgccaaaat
     8641 gtcaagttgc atttgtagat ggttatgaaa aagctgggcc ttttggtggt aagagtattg
     8701 gagaatgtgc cgttgtacca gttgcgccag ctgttgcaaa tgcagtatat aatgcaactg
     8761 atgtagagat acattcatta ccaataaaat taaaatagta gaaattaaag cagaaataac
     8821 aatttattgt atttgtaaat agataataaa aatagattta tttggtgagg agatattatt
     8881 atggcaaaga tattaggtat ttgtggaagt ccacgtaaag gagcaacaga atatgcagtg
     8941 ttagaagctt tgaaagaagc agaaactata cctggtatag aaactgagta ttggtcagtt
     9001 agagggaaaa aaataagtcc ttgtgtacat tgtgatgcat gtataagaaa agaaactatg
     9061 tgtattataa aagatgatat ccaagaatta gagcaaaaaa tattggaggc agatggaatt
     9121 atagtaggtt ctccagttta tgatatgaat ataactgctc aacttacagc agtatttaat
     9181 agacttcgtc caatgtattt agttcatcca ggaaaacttc aaaataaggt tggtgcagct
     9241 ataactactg gtggaactag atatggtggc caagaattga caaaacttcc tataataaat
     9301 ttcttcctaa tgcatgaaat gttagtgtct ggaggtttag gtggatgcta tataggtggt
     9361 acaatatgga gtaaagatgg aaaagcaaaa ggtgcccaag aggatgagac aggtatggac
     9421 acagtaaaaa gacttggaaa aggtgcagca gaggcagtta tggtttctaa gtttggtctt
     9481 gaaaaatgga atgtcgtaaa agaagaatta gatgttaaaa aagatgaaaa atcacctctt
     9541 agagaccact aaagatttat tatattattg aatatataca aatagtgatg taatagatta
     9601 ttgcatcact attttatgtt ttaatttaga ttttataaat tttatattaa aaaatataga
     9661 aacaaagtgt tttgagattt atttttaaat tttaaaaatg agaattaaaa agtgagaaga
     9721 tagaaaaggt tttttagtta ttaaattcta catacattgt atgaactaaa gatagaaaag
     9781 aaaaaataat aaaagcattg caatttctat actaaagagt catggaaaaa tatctagtat
     9841 atatttagta ttggcatgtt aattgcttat atattaaagt ataaaaattt aacaataatt
     9901 tggaggtatt ttagatgtta aaagaaatta aatggaaagt aaataatttg ccaaagggcg
     9961 ataaggaaaa ttgtatcaaa tttttaaatg aagaagaaat aactaaggtt agaaattttc
    10021 acaaaagctt tcctcaatat aaagaaactc cattagcaaa cctagaaggt ttagcaaaaa
    10081 aattaggtgt tgcaggagta tatgtaaaag atgaatctta tagatttgga ttaaatgcat
    10141 ttaaagtatt aggtggttct tattctatgg gtagatactt agctcaaaga ttagatacag
    10201 atatatctga gttagggtat gacaaattga cttcgaaaga aataaaagaa aaattaggag
    10261 aaataacttt ctttacagct acagatggaa atcacggtag aggagttgct tggacagcta
    10321 ataaattagg tcaaaaatct gttgtattaa tgccaaaagg ttcttctgaa tttagattaa
    10381 ataaaattaa aggagaaggt gcagatgcta gtataacaga cttaaattat gatgatgcag
    10441 taagattagc taatgattat gcagaagctg atgaccatgg agtaatggtt caagatacag
    10501 catgggacgg atatgaagaa ataccagcat ggataatgca aggatatgga actatggctc
    10561 aagaagctat agagcaatta aaagaatatg gagtagatag accaacacac gtatttgttc
    10621 aagcaggagt tggttcactt gctggtgcag ttcaagggta tgttgcttca atatatgatg
    10681 aatgcccaat aacagtagta gtagaggctg atgaagctga ttgttactat aaatcagcag
    10741 aagctggaga tggaaaacca agatttgttg gaggagatat gccaactata atggcagggc
    10801 ttgcttgtgg agagccaaat actataggat ttgaagtatt aaaaaatcat gcagcagcat
    10861 ttgtttcagc accagactgg gtttctgcta aaggtatgag aacattaggt aatcctttaa
    10921 atggtgatga aaaggtaatt tctggagaat ctggagcagt tacaactggt cttttagtag
    10981 cagctatgga aagagaagat ttagcagatt taagaaaaga tttaaaatta gatgaaaatt
    11041 caagaatact tttaataagc acagaaggag atactgaccc tgacaaatat agaagtatag
    11101 tttgggatgg agaatatcca agtatataag gaattactaa aatgcaatta ttagatattg
    11161 gtttacatat ttagaagata tataagttct aaaatttaga aaatatataa gtatataaaa
    11221 tacatacatt ataaaaaatc tagggggata atgaaatgtt aaacgaacaa agaaaacaag
    11281 aagtaactga aatatgccaa aaattaattc aaaatcctag ttcttctgga aatgaagaag
    11341 gtgttgtaaa agcaatagaa gagtcttttg aaaaattagg ttttgatagt tggtcaagag
    11401 atagatatgg aaatatagta ggatgtataa aaggtaacaa gccaggtaag aaaatattat
    11461 ttgatggaca tatagataca gttccagttc cagatgcaag taaatggagt gttcctcctt
    11521 ttgaaggaaa aatagttgat ggaaagatat atggtagagg aacttctgac atgaaaggtc
    11581 aatatactgc aatgatgtct gctgtagcgt attttgctaa agacactaat agagattttg
    11641 ctggagaact atatgtagca ggtgttgttc atgaagaaat atttgaaggt gtttcggcta
    11701 gagagataag taaggctgtt caacctgact atgttgtaat aggtgagtct tcagagttaa
    11761 acttaaagat tggtcagaga ggtagaggag aaatagtagt agaaactttt ggtaaaccag
    11821 ctcactctgc taatccagaa aaaggt
//
LOCUS       DAYAOX010000115        11450 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.115, whole genome shotgun sequence.
ACCESSION   DAYAOX010000115 DAYAOX010000000
VERSION     DAYAOX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.115"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(138..1568)
                     /gene="dcm"
                     /locus_tag="ACGI57_002390"
     CDS             complement(138..1568)
                     /gene="dcm"
                     /locus_tag="ACGI57_002390"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFG9717465.1"
                     /translation="MKKITIDKNRVRKIMKEKNIRSQNQLALNMGITKNQLSAILSEK
                     FTPIKSNVEKLCEVLDIGFDEIMCLEDVNEKQLKFTNICSSNKDYIEHRDCTYKELSE
                     YELNPRERNKNNKHIDISNVLAKRAYTCVELFAGAGGLALGLEKAGFKEVGLVEWDKY
                     ACDTLKLNRPDWNVIQGDIVKIAENGIKNYIDNNIEIDLLSGGYPCQAFSYAGKKLGL
                     EDVRGTLFYSYAKILEELKPKVFLAENVRGLVSHDGGKTLKTMIDVFSEIGYNVKYEI
                     LKAVNYGVAQKRERVIIIGTRKDLSHVDFKFPKSFEYVATLRDALKNVPKSEGARYPE
                     RKKKVLDMVPPGGCWIDLPDEVARDYMGKSYYSGGGKRGMARRISWDEPCLTLTCSPA
                     QKQTERCHPDETRPFTTREYARIQSFPDEWEFTGSVSQVYKQIGNAVPVKLGKAIGLS
                     IVDYLNKIEKIYVTEEVAITEEEYVI"
     gene            1781..2497
                     /locus_tag="ACGI57_002391"
     CDS             1781..2497
                     /locus_tag="ACGI57_002391"
                     /inference="COORDINATES: protein motif:HMM:NF025864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HFG9717466.1"
                     /translation="MTELEKQEILDKAKDFFTNILVANHKKNTIKLNLKAFNINPFLV
                     KYLASFLTGSNKPEDIAKALIYPRVLGTSINTSFGTNLQKFCITTLDGFASTTSGIDI
                     EFIDCIDGRRKFCQIKAGPNTINKDDVKTIIDHFSSVKRLARTNGLSLSYNDLIVGVF
                     YGTENELNAHYKRINEEYPVVVGQDFWYRLTGDENFYFDLIDTIAQSALEEDCTVLLK
                     KTISNLADEISKDKSLFNQK"
     gene            complement(2742..3020)
                     /locus_tag="ACGI57_002392"
     CDS             complement(2742..3020)
                     /locus_tag="ACGI57_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6809 family protein"
                     /protein_id="HFG9717467.1"
                     /translation="MYDEIIEIYFDYWINEHMYRKVLEKDEYYNDRCKEIKKVNKLLE
                     KNLSEEKKMDVLIELNDLFNSVIEIERELGYKLGFKAGARFMIELLNS"
     gene            complement(3268..4602)
                     /locus_tag="ACGI57_002393"
     CDS             complement(3268..4602)
                     /locus_tag="ACGI57_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFG9717468.1"
                     /translation="MDNMSNLVKNTEAEQSILGSILLDSRKIEEIEILISEDMFYHQE
                     HKHIFNSVKDIHKRDEPIDIITLTEDLKKKGLIDYVGGISYLTKLSTIVPTIGNVSHY
                     IEIVIEKYKNRNIIDKFNKFKLGEIDDNTLIYQLEKYVNEVNQKLVKKDSSITAISER
                     AFEYINDEIEEGIDIGIKFLDETIGGLYSGELITIAAKSGRGKTALALQIMRNVVFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNEEEIIKYINALSTLNQENNLY
                     INDSVNTISGIKREIKSIKPDVVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPIIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALDYCKTMGIE
                     DIKELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGSLFSFKECKKVKEV"
     gene            complement(4615..5358)
                     /locus_tag="ACGI57_002394"
     CDS             complement(4615..5358)
                     /locus_tag="ACGI57_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717469.1"
                     /translation="MSNLGWIKLHRKIKESPLYKDCNSKQRDILINLLLSVNHKPNKW
                     VYGGKEYEVKSGQLITSLESIANMCSKDVSIQNVRTALKKFERYGFLTNKSTNKNRLI
                     TIENWELYQSNEENQQTNQHAINKQLTTNKNVNNEKSKIYSEIIEKFNITCTELSKVK
                     KLTEARKKVINARIKEYDKETVLEIIHIVSKTSFLNGSNEKGWKANFDWIMSPNNFIK
                     ILEGNYQDKSINVKVDAEAYSPDDSQLKW"
     gene            complement(5379..5693)
                     /locus_tag="ACGI57_002395"
     CDS             complement(5379..5693)
                     /locus_tag="ACGI57_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717470.1"
                     /translation="MADTNNLEVLKTLQATLNILVKQEQEAQEKEKGYKEIQTYRRDK
                     AQLITCKEASKIYPIGESKFRQLCHSKNKGFPCIWIDNRVYIIKNKLDNWFIENANGI
                     KL"
     gene            complement(5697..5891)
                     /locus_tag="ACGI57_002396"
     CDS             complement(5697..5891)
                     /locus_tag="ACGI57_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9717471.1"
                     /translation="MKLKLKRIEKGIKQYELAEKVGISRYYLSSLERGKSKNPSNMLM
                     LKLAQELDTSVEELFFDKEE"
     gene            6031..6891
                     /locus_tag="ACGI57_002397"
     CDS             6031..6891
                     /locus_tag="ACGI57_002397"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9717472.1"
                     /translation="MIDNTIGKKIKQYRKSINMSRDELANKIGCSVYAVAKYEQGQRK
                     PSLDMIKKISTALSIPINRLIIDESDLDTSTNNYTNLKELDLVVKTFDNYSAKLIGNN
                     ISKFRNINNISLKELSEKINCSENEIINYENGTKMPRIEILKKIALATYTKIEDLFGF
                     PLEEIKIDNQTILYRNPQTGYIIDNFLIDFINKDINIDNTPVSYYIDKSEFDLVIHIL
                     KNYGYDVKTDYKTKFEIADNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSN
                     GLIEDEDEEY"
     gene            6988..7740
                     /locus_tag="ACGI57_002398"
     CDS             6988..7740
                     /locus_tag="ACGI57_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFG9717473.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVSDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDEYNYKSYKELFKNGN"
     gene            7730..8896
                     /locus_tag="ACGI57_002399"
     CDS             7730..8896
                     /locus_tag="ACGI57_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9717474.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNSDILKAGELYRNDNYVFCNELGAPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKIEAVEKLDKIFSL"
     gene            complement(9013..9243)
                     /locus_tag="ACGI57_002400"
     CDS             complement(9013..9243)
                     /locus_tag="ACGI57_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717475.1"
                     /translation="MEANKTLLQSLYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGISDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(9492..>11450)
                     /locus_tag="ACGI57_002401"
     CDS             complement(9492..>11450)
                     /locus_tag="ACGI57_002401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717476.1"
                     /translation="FYNVLILISTKVNHTLNADYIVKYILQNTMADRDAIFIPIFNEV
                     YEEEESSIKRLLGWCFDFEGFDNTSNETIRLSAMMIATFLISSNRDLRDKSTKALVNL
                     LTGKMDIVIDLLKTFEDVDDPYIIERLYAVAFGCVVSEQSEEMIEALAVYVYNKIFDV
                     DYVYPNILLRDYAKNIVDYAKYKIDSSVLKDMNVEPPYKSIFPKVPTDEEIEGYKYDY
                     KASDFKDYYWSQNTILNSMEVEYTRDGQPGWYGDFGRYMFQHYFHKWKGLDYNDLKNI
                     AIKKVFDMGYDVNKHGKFDRTVQNGRLRDSKYERIGKKYQWIALYELAAQAADNYKIE
                     VYTDAYGTKKEIYCKGTFEPDIRNIDPTVMTVINNNNNKRVHNQLFQFSPIPNNEWLG
                     DFSDLPSINDMVNSVYKNQDFLLLNGWYTWKEENDFKNKNYKNPEKDMWVLINSYIIK
                     NDQLEPIVNALKGKDFMGRWLTEPNSNYTLYNKEYYWADGYYFFQNSYYCGEEWVQID
                     EHKNILEKDYKVLLPTYSYTTEREGDIFQEDFSGTWHKPCSTLFYGLKLRYGQGDSIL
                     YNDYGEVVCFESSELLNEEIGFFINKEIFLKYLNEQGYSVFWTMLAEKRVLEEPYMDR
                     KYIQPTISGVYTLNGENRLVGETIESEY"
BASE COUNT         3947 a         1853 c         1247 g         4403 t
ORIGIN      
        1 aagtacataa taaaattaaa gagagcaatt ttttttgctc tctttcaaaa tttatattaa
       61 attgtaataa taaaaataaa aacgctagtt atctaaggct aatattttca acgattatat
      121 aaatatctac ataacagtta aattacatac tcttcttcag ttattgcaac ttcctcagtg
      181 acatatattt tttctatttt attaagataa tctactatac ttaatccaat agcctttcct
      241 agcttaacag gaactgcatt tcctatctgc ttgtacacct gactaactga ccctgtaaac
      301 tcccactcat caggaaaact ttgaattcta gcatactctc tagttgtgaa tggtctagtc
      361 tcatctggat gacacctttc agtttgcttt tgtgcaggac tacatgttaa agttaggcat
      421 ggttcatccc aacttattcg ccttgccata cctctttttc cgccaccgct ataataactt
      481 tttcccatat aatctcttgc cacctcatct ggcaaatcta tccaacaccc acctggtggt
      541 accatatcta acactttttt cttcctctca ggatatctag ctccttccga ttttggaaca
      601 tttttaagtg catctcttaa cgtagcaaca tattcaaacg acttagggaa cttaaaatcc
      661 acatgtgaca aatcttttct tgttcctatt ataataactc tttccctctt ttgtgcaact
      721 ccatagttca cagcctttag tatctcatat tttacattgt atccaatctc gctaaataca
      781 tctatcatag tctttaatgt ttttccacca tcatgagata caagacctct aacattctct
      841 gctaaaaaca ccttaggctt taactcttct agaattttag catatgaata aaacaatgtc
      901 cctcttacat cctctaaccc tagcttctta cccgcatagc taaatgcttg gcatggataa
      961 ccacctgata gcaaatctat ctctatatta ttatcaatat aattctttat tccattttcc
     1021 gctattttaa caatgtcacc ctgtataaca ttccagtctg gtctatttaa ttttaatgta
     1081 tcacaagcat atttatccca ttctactaat ccaacctctt taaaaccagc tttttcaagg
     1141 cctaatgcta atcctcctgc tcctgcaaat aattcaacac atgtatatgc tcttttagct
     1201 agtacattac ttatatctat atgtttatta tttttatttc tctctctagg atttaattca
     1261 tattcactga gttctttata tgtacagtct ctatgctcta tataatcttt attactgcta
     1321 caaatatttg taaatttcag ttgcttttca tttacatctt ccaaacacat aatttcatcg
     1381 aatccaatat ctaacacttc acataatttt tctacattac tttttatagg tgtaaatttt
     1441 tctgataata ttgcagataa ctgattctta gttatgccca tatttaatgc cagttgattc
     1501 tgacttctta tatttttctc tttcataatt tttctcactc tatttttatc aatagttatt
     1561 tttttcattt agttcacctc ttaataaata atataccctt aaatagttaa taaaatacat
     1621 taactattta aggatatatt atcataattc taatttttat gtaagtagtt ataaaaactt
     1681 aaaataaaat atctgtaaac aacctaatat tacctcataa ttttacacat tatggaatat
     1741 attgtataat tacattaata ctttttaggg ggattatttt atgactgaat tagaaaaaca
     1801 agaaatacta gataaagcca aagatttttt tacaaatatt ttggttgcta atcataaaaa
     1861 gaatactatt aagttaaatt taaaggcatt taatataaat ccttttttag ttaaatactt
     1921 agctagtttc ttaacaggat caaataaacc tgaagatatt gctaaagcat tgatttatcc
     1981 aagagttcta ggcacttcga ttaatacgtc cttcggaact aatttacaaa aattctgtat
     2041 taccacttta gatggttttg cctctacaac ttctggcatt gatatagagt ttattgattg
     2101 tattgatggt agaaggaaat tctgtcaaat taaagctgga ccaaacacta ttaataaaga
     2161 cgatgttaaa actataattg accactttag ctcagttaaa agacttgcta gaacaaatgg
     2221 actttcatta tcttataatg acttaattgt tggtgttttt tatggaacag aaaatgagct
     2281 taatgctcac tataaaagaa ttaatgaaga gtatccagta gttgttggtc aagatttttg
     2341 gtataggctt actggtgatg aaaattttta ttttgattta atagatacaa ttgctcaatc
     2401 tgctttagaa gaggactgta ctgttttact taaaaaaaca atatccaatc tagctgatga
     2461 aatttctaaa gataaatctt tattcaatca aaagtaatat aaaaatgatt aaatagcata
     2521 atttacattt ttagatattt aaaaataata ttcaccaaac aaaattatta agtaaaatga
     2581 aatgtatctt ataaaagctg agctataagt aaatctttgt aaaaacaaat ttacttatag
     2641 cttttttatg ttcaaaaaac acaaattata tactatttaa tatattataa acttttattt
     2701 gttaatactc tctactaaat ttctaacatc tcttatataa attaactatt taataattca
     2761 atcataaatc tagctcctgc cttaaaacca agcttataac caagctctct ttcaatctct
     2821 ataactgaat taaataaatc atttaactct atcaatacat ccattttctt ttcttctgat
     2881 aaattttttt ctagtaattt attaactttc ttaatttctt tacatctatc attataatac
     2941 tcatcttttt ctagtacttt cctatacatg tgttcattta tccaataatc aaaatatatt
     3001 tctataattt catcatacat gttagtacct ccttgacttt catctggtac ttaatatata
     3061 cttaacttgt gtagattagt gtacattaag tacctaattt ataaaggtag ttgttatatt
     3121 tatatggcaa ctaccttttt ctaataaatt tttcaatcta aatactatat attttaaatc
     3181 ttcattgctt actattgagg attatatact tttcttctaa cactccttaa acaatagttt
     3241 atatatcact taataaatta tattttttca tacctccttt accttcttac attccttaaa
     3301 agaaaacaaa cttcctatat accaataagg tctaaagcct gattccccat ctctattttt
     3361 agcaactata atttcactta aattagctcc aaaacctttt tctctacgtc ttaattcttt
     3421 tatatcctct attcccattg tcttacaata atcaagagcc tctttctctg taggtctatg
     3481 aatatatatg actgaattag aatccatata tatctgtttt gaacttctca ttactctatc
     3541 tccttttggc ctttcatcct tcaattcatc atttaattga gccaactgta taattggaat
     3601 attaaagtct aaagtaatct tttttatttg ccttgataaa tcagcaaact tagcttctcc
     3661 aacttcttta ccttcgtaat ccaaaatctg aagataatca attataacaa catctggttt
     3721 aatacttttt atttctctct taattcctga tattgtattg actgaatcat taatatagag
     3781 attattttcc tgatttaaag tacttaaagc atttatgtat ttaattattt cttcttcatt
     3841 tagatttcta ttcttcattt ttacaactga tattccagtt aaagaagcta tgtttctaaa
     3901 caaaatttgt atatcactca tttccccact tataaaaagt acttttttac cttgaaatac
     3961 cacatttctc attatctgta aggctagtgc tgtttttcct cttccacttt tggctgctat
     4021 tgtaattaat tcaccactat atagacctcc aatagtttca tctaaaaatt ttattcctat
     4081 atcgatacct tcttcaattt catcatttat gtattcaaag gctctttctg atatagctgt
     4141 tatactagaa tccttcttaa ccaatttctg attaacttca ttaacgtatt tttctaattg
     4201 atatataagt gtattatcat ctatttcacc taatttgaat ttattaaatt tatcaattat
     4261 atttctattt ttatattttt caataactat ctcaatataa tgggatacat tacctatagt
     4321 tggaactatt gttgatagtt ttgtcaaata acttatacct ccaacataat ctataagccc
     4381 ctttttcttt aaatcttctg taagagttat tatatctata ggctcatccc ttttatgtat
     4441 atctttcact gaattgaata tatgtttgtg ctcttgatga taaaacatat cttcacttat
     4501 taatatctca atctcttcta ttttcctaga atctagtaaa atactaccta atatagattg
     4561 ttctgcttct gtgttcttaa ctaaattact catattatcc ataatataaa ctccctacca
     4621 ctttaattgt gaatcatctg gactatatgc ttctgcatct actttgacat taattgattt
     4681 atcttgataa ttgccttcta atatcttaat aaaattattt gggctcatta tccaatcaaa
     4741 atttgccttc caaccttttt cattgcttcc atttaaaaag ctagttttac taactatatg
     4801 aataatttct aatacagtct ctttatcata ttcttttatt ctagcattaa ttactttttt
     4861 tctagcttct gtaagtttct ttacttttga tagttctgta caagtaatat tgaatttttc
     4921 tataatttca ctgtatattt tacttttttc attatttaca ttcttgttag ttgttagttg
     4981 cttgttaatt gcatgttgat tagtctgttg attttcttca ttagattgat atagctccca
     5041 gttttcaata gttataagcc tatttttgtt tgttgattta tttgttagaa atccatatct
     5101 ttcaaatttc tttaaagcag ttcttacatt ctgaatactt acatcttttg aacacatatt
     5161 tgctatactt tccaaagatg ttattaactg tcctgattta acttcatatt ctttacctcc
     5221 ataaacccat ttattaggct tatgattaac tgacagtaat aggtttatga gtatatctct
     5281 ttgtttggaa ttacaatctt tatatagtgg gctttctttt atctttctat gaagctttat
     5341 ccaacctaga ttactcaaat actatcaccc acttaagtct ataattttat accatttgca
     5401 ttttcaataa accaattatc tagtttattt tttattatat acactctatt atctatccat
     5461 atacaaggaa atcctttatt tttagaatga cataactgtc taaattttga ttcccctatg
     5521 ggataaattt tagaagcttc tttacaagtg ataagttgag ctttatctct tctatatgtc
     5581 tgtatctcct tgtacccttt ttccttttct tgtgcttctt gttcttgctt gacaagtata
     5641 ttcaatgtag cttgtagtgt tttaagaact tctagattat ttgtatctgc catacatcat
     5701 tcctccttat caaaaaataa ttcttctaca cttgtatcta gttcttgagc caattttaac
     5761 atcaacatat tacttggatt ttttgattta cctctttcta aagaagataa ataatatctt
     5821 gagatgccaa ctttttcagc taattcatat tgctttatac ctttttcaat tctttttaat
     5881 ttgagtttca ttgatttcct cctttctttc atacccattt tgtgtatcct catttctata
     5941 aaatattcta acacccattt ggtgtattgt caatatattt aagtgtatca atagacccat
     6001 ttagtgttat aatgtatttg aggtgaaatt atgatagata atacaattgg aaaaaagatt
     6061 aaacaatata gaaaatctat aaatatgtca agggatgagt tagctaataa gattggttgc
     6121 tcagtttatg ctgtagctaa atatgagcaa ggccaaagaa agcctagttt agatatgata
     6181 aaaaaaatat ctactgcatt atcaattcct ataaataggc ttattattga tgaaagtgat
     6241 ttagatactt caacaaataa ttatactaat ttaaaagaat tggatttagt tgtaaaaaca
     6301 tttgataatt actcagcaaa actcatcgga aataatataa gcaaatttag aaatataaat
     6361 aatatatctc tcaaagaact ctcagaaaaa ataaattgtt ctgagaatga aattataaat
     6421 tatgagaatg gaacaaaaat gcctagaatt gaaatattaa aaaaaatagc acttgctact
     6481 tatactaaga ttgaagactt atttggattt ccattagaag agataaaaat agataaccaa
     6541 acaatcctct atagaaatcc ccagactgga tatattatag ataactttct tatcgatttt
     6601 ataaataaag atattaacat agataatact ccagtttctt attatataga taagtcagaa
     6661 tttgatttag taattcatat attaaaaaat tatggttatg atgtaaaaac agattataag
     6721 acaaagtttg aaattgcaga taatgcagat acaataaaac caattagctt aagcttctct
     6781 gacttcaaag acttctccaa aaatctaaat tgggtgataa atggtcttgt tgaaaatttt
     6841 atacaagaaa actctaatgg tcttattgaa gatgaggatg aagaatatta gtattgcaca
     6901 ttttcaaaat atgagtttac ctattaactt ttatacattc atagttaata aataaaaatt
     6961 agatatgata tgaaaggagc taaaatcatg aataaatcat atataccaaa atacacacat
     7021 aaattcccta ctaatctacc tcatggagat aaaaaatata ctttaacata cattagaaga
     7081 atgataagtg aatttgtata tgatatgggt aacctagaaa ataacccctt tacattccca
     7141 gaagtacaaa cattacttga tggtataacc gtaggtggac ataaattaag tgaccaaaat
     7201 caaatactaa atataaaatc atcatgggat tatgtaataa aattaagtaa caaagaaaac
     7261 ttatctttaa ataaagatac aatttgctct atacataaaa tagttgcaaa agatgaagct
     7321 ctaatagttg gagattttag aaatggtaac ataggtatag ctggtactac tcaatatgaa
     7381 tgcatagaag ccactctatt aaatgattta tttgtaagcg acataaatat cataaataaa
     7441 ataactaacc ccttagaaaa ggctataata ataaatttat ggttgtccta ttgccaattc
     7501 ttctatgatg gcaacaaaag aactgctaga cttacctcaa acctaatact tataagtaat
     7561 gatataggag ttctatcaat accagctaaa cataaacaag aatacaacac attaatgcta
     7621 aacttttatg aaacactaga agctgatgaa gttataaaat tcttattaga gaaatgtatt
     7681 acattctttg atgaatataa ctataaatca tataaggagc tgtttaaaaa tggcaactaa
     7741 aagagctaat ggagaaggct ctatcggaaa atataaagat ggttggcgtt cccgtataat
     7801 gattggctat aatgaagatg gtaaaccaat aagaaaagaa ttttatggta agactcaaaa
     7861 ggaagttaaa gataaacttg atgcttataa aaaacaatat tatttaagct ctgatatttc
     7921 agatgataaa ataactttag agcattggtt ttacaattgg ttatttgaat atagaataaa
     7981 agatttaaaa cctaaatcat ttgaaagata tgaaagcatc tatcgtaatt atattaaaga
     8041 tactgattta ggcaatgtta aattaaagga cttaagagta tctcacatac aaaaatatta
     8101 caatagattg cttgattcca ataaaagtat acctactata aggcaaataa acactaaact
     8161 gaaaacctgc ttatcagaag ctgaaaaaca aggtcttata caacgtaatt attgtactat
     8221 ggtaaatata ccagttgaga aaaaggagaa taagcttgaa atattgactc ttgaacaaca
     8281 aaagagtttt ataaaagcta tagatggaca taaattagag gtattatttt tacttgctct
     8341 tggtactgga cttagattag gagaattatt aggacttaaa tggtttgata ttgattttaa
     8401 aaagtctaat ttaacagtta aaagaaccct tcaaagaaca tattttatag ataaaaccgg
     8461 aaatagagag cttaaagtat tagagcaaga gccaaagaca tcaaattctt acagaactgt
     8521 acctattcca aaagatgttt taaataaatt gaaagagcat aaagtaaatc aaaattcgga
     8581 tatattaaag gctggagaat tatatagaaa tgataattat gtattttgca atgaattagg
     8641 tgcccctatt gatgataaaa gacctttaag gaatctaaaa tctatattaa acagcttaga
     8701 tatagagcct ataaaatttc atggtcttag gaaaacttat gcgactagac tttttgagaa
     8761 tgatgtccct cctaaaactg tacaagtttt aatgggtcat tatgatatat caataacttt
     8821 aaatatatat actcaagtta tggaagataa gaaaattgaa gctgtagaaa agttggataa
     8881 aatattttca ttgtaaaaat caaatatata aaaatactct gatttcatat agttaattac
     8941 aaaaccaaaa taaggataca aattaatttt atattatctt gtatccttat tcttgtctag
     9001 ttttttaaat atttatcttg gatagccttt atgatacatt aatccatttt ctaacattag
     9061 ttcttgggct aaatatatat gacccttaca gtgcatatct gatataccct cacttataag
     9121 tttatatgtt tcagaagtat ataaataatc catactttct tccaagctct tacctgtttg
     9181 tttagaaaat tctaatatga tttttttata tagcgattgt aacaatgtct tattagcttc
     9241 cataaaagct atacctcctc tttcaaatat ttgtttatcc tattttgact aactatatat
     9301 ttattagtta tttaattagc cataataatt cggctataag atatttataa tctaatctca
     9361 attatataaa tctttttgcc aatatttaat aatcaaaata ttgcacttaa ttaactatct
     9421 tataagtatt gataataaaa tctgtcttct taaatatact taaataaaac ttctataatt
     9481 atatcaaagt attaatattc actctctatt gtctctccta caagtctatt ttctccattt
     9541 agggtatata ctcctgatat agttggttgt atgtattttc tatccatata tggttcttct
     9601 aaaactcttt tttctgctag catcgtccaa aatacactat atccttgttc attaagatat
     9661 tttaagaaaa tttccttatt aataaagaat cctatctctt cattaagtaa ttcactactt
     9721 tcaaaacaaa caacttcacc ataatcatta tatagaatcg aatctccctg accatatctc
     9781 aatttcaacc cataaaatag agtactacaa ggtttatgcc atgtgccaga aaagtcttct
     9841 tgaaatatgt caccttctcg ttcagtagta taactataag ttggcaataa tactttataa
     9901 tccttttcta agatattctt atgttcatct atttgtaccc actcttctcc acaataatat
     9961 gaattttgaa aaaagtaata tccatctgcc caataatatt ccttattata aagagtataa
    10021 ttactattag gttcagtaag ccatctaccc ataaagtctt ttccctttag agcattcact
    10081 attggttcta gttggtcatt tttaattata tagctattga ttaataccca catgtctttt
    10141 tcaggatttt tatagttctt atttttaaaa tcattttcct ccttccaagt ataccatcca
    10201 ttaagtaata aaaagtcttg atttttgtaa acactattta ccatatcatt tatgcttggt
    10261 aaatcactaa aatctcctaa ccactcatta ttaggtattg gagaaaattg aaataattga
    10321 ttatgtactc ttttattgtt attattattt attactgtca tgacagttgg atcaatattc
    10381 ctaatatctg gctcaaatgt acccttacaa taaatttctt ttttagtacc ataagcatct
    10441 gtatacactt ctatcttata attatcagca gcttgcgctg caagttcata taatgcaatc
    10501 cattgatatt tctttcctat tctttcatat ttactatctc ttaatctacc attttgtact
    10561 gttctatcaa atttaccatg tttatttaca tcatatccca tatcaaaaac tttcttaata
    10621 gctatattct ttaaatcgtt atagtctaga cctttccatt tatggaaata gtgttggaac
    10681 atatatcttc caaaatctcc ataccatcct ggttgtccat ctctagtata ttctacttcc
    10741 atagaattta agatagtgtt ttgactccaa taataatctt taaaatctga tgccttatag
    10801 tcatacttat atccctcaat ttcttcatca gttggtactt taggaaaaat tgatttatat
    10861 ggtggttcaa cattcatatc ttttaatact gaactatcaa ttttatattt tgcataatct
    10921 acaatatttt ttgcataatc tcttaataaa atattgggat aaacataatc tacatcaaat
    10981 attttattat aaacataaac tgccaatgcc tctatcatct cttctgattg ctctgataca
    11041 acacatccaa atgcaactgc atataatcgt tcaataatat atgggtcatc tacatcttca
    11101 aatgttttaa gtaaatctat tacaatatcc attttccctg ttaatagatt tacaagagct
    11161 tttgtacttt tatctcttaa gtctctattt gaagaaatta aaaacgttgc aatcatcata
    11221 gctgacaatc taattgtttc atttgaagta ttatcaaaac cttcaaaatc aaagcaccat
    11281 cctaataatc gcttaataga agactcttct tcttcataaa cttcattaaa tataggtata
    11341 aagatagcat ctctatctgc catagtattt tgtaaaatat attttactat ataatctgca
    11401 tttaatgtat gattaacctt tgtagaaatt aagataagta cattataaaa
//
LOCUS       DAYAOX010000116        11246 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.116, whole genome shotgun sequence.
ACCESSION   DAYAOX010000116 DAYAOX010000000
VERSION     DAYAOX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.116"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            461..2272
                     /locus_tag="ACGI57_002402"
     CDS             461..2272
                     /locus_tag="ACGI57_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9717477.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVATVLSKTFNIKETCARICSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2360..2941)
                     /locus_tag="ACGI57_002403"
     CDS             complement(2360..2941)
                     /locus_tag="ACGI57_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717478.1"
                     /translation="MSYLLKINQFERSYDIGIFKNEQSIYKFIEKIPFVKKDSSNFNY
                     TNYYMEFEAIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
     gene            complement(3377..3583)
                     /locus_tag="ACGI57_002404"
     CDS             complement(3377..3583)
                     /locus_tag="ACGI57_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717479.1"
                     /translation="MNQSQEKDLEFIKNHWDLTIIEKERNVTRIIRYSLIILAIIAFF
                     LPTKIKFSIYIFISLFCWLMYLVL"
     gene            4127..4822
                     /gene="cas6"
                     /locus_tag="ACGI57_002405"
     CDS             4127..4822
                     /gene="cas6"
                     /locus_tag="ACGI57_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HFG9717480.1"
                     /translation="MIKCLEMSVKVYLLENISLDNTSKEIRKLIDDTLIIEEKYKEFH
                     NKAGFKNYSFNLLYPLEKDKVYKKDCIYTFIIRTVDSELGMYFMNNLKNQHSDKIKVI
                     TMSKKVIPKKHIESIYSVTPIIAKFEDGYWRSNKTVEDLEKRIRENLIKKYNEFTNSK
                     IDEDFELFQVIKFINRKPISTKYKNISLLGDKISFKVSENDMAQEIANFALGVGVGEI
                     NSRGFGFVNYRWL"
     gene            4834..6573
                     /locus_tag="ACGI57_002406"
     CDS             4834..6573
                     /locus_tag="ACGI57_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HFG9717481.1"
                     /translation="MLKDCLEIFEKEYKSLGNRLITDSYVLSYGTYILVKNFSNIDIL
                     EVSKNNVDSTEDIYHKFCELDYLSKLVSIDKSVDSKKIIHSNNYLSFFVKKENLRPDK
                     NNNVKLTEEIIDNYYDALLNPRIKYKSDKRKKEIYDSIENKYGKVDENKLNKIRLWIK
                     TNIFDLVESVKKDKNYLKIFFDEKIELYNDENQKYLYPNIYNKNDFNVIIDGKNYGLP
                     NDNMQLNEKKPFLKLKTRIRNSTPYLISDDEVLLQKAFFDYLTNKAANGEEVIYISSE
                     KGIHRKTDIPNEFSGYILRIKKGKELEIHDFDTVVNLNTKIKRINVECIIPIEYERHN
                     LKPNLEIGYINNLIKLEKTIDTVFFNKFMCNSYFKEIKNINLSDLVLRDILIRHREGL
                     YTWFYKGRDSIVKQTFSNYSMGIIENSIKNNNYIRAKEQYNLRIAIINYFHGGSNMAD
                     KIKVISESLFEKINSIKTKCIQSDEEYYFAVGQLASYFISKNKSKNKNHSLINPILNA
                     QNRKKIMLEIEKLFKKYNYDISYDNNRRFKNLYSMIVGYEVEEKKVNRDLLIGGYLYY
                     NLIYTKSEENENE"
     gene            6566..7555
                     /locus_tag="ACGI57_002407"
     CDS             6566..7555
                     /locus_tag="ACGI57_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HFG9717482.1"
                     /translation="MNRRVYGVLGIKSIMSNWNADFSGYPKTTSTGEVFGSDKALKFP
                     MKKMWENEGEKVLYVKSLKIDESKKGEIKIRPKSLKERYEELFDIEDLKLEKNQQKVL
                     ENLFKAIDVKNFGATFAEEGNNIGIMGAVQIGQGFNKYEDINVEEQQILSPFRDPKAK
                     DKKKSTNEEEKEDANSTTLGKKIVSDEAHYFYPFSINPKAYEVFVNMGVTDGYTKNDY
                     KKFKEAALVSATAFNTNSKLGCENEFALFVETDEELYLPDLAQYITFEKREKSKDLIC
                     INIIHLIAELKDDIKSVEIYYNPYKIEIKPFKDEILPKIDKVKFMNIFTRKEV"
     gene            7557..8297
                     /gene="cas5b"
                     /locus_tag="ACGI57_002408"
     CDS             7557..8297
                     /gene="cas5b"
                     /locus_tag="ACGI57_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5b"
                     /protein_id="HFG9717483.1"
                     /translation="MEVLKFNLSGKTAFFKKPDVNSIIYFTYGNIHKIALMGILGACL
                     GLGGYNQQSKEDEYPEFYSLLKDIKMSIVPKHEFGYINKKVHIFNNSVGYASKEEGGN
                     LIIKEQWLENVSWDIYIQLEDSTTSRELKRRFLSNKFVYIPYLGKNDHIANISEIEII
                     TDFYKTEDINRVDSFFIREDFEFEKSYFDDNDDYKEPFKYQEKLPYRLHRMTNQYILE
                     TIIFTNMSVKLINKKEVYQVKGLNLIFI"
     gene            8321..10879
                     /locus_tag="ACGI57_002409"
     CDS             8321..10879
                     /locus_tag="ACGI57_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFG9717484.1"
                     /translation="MYFKKVELFDISNNINNCKNIYAHINKDRKPETLKEHSDLSYSY
                     LMKLCVKKDLSSCLERIENKFFKKDSFLSKNFYREIILNMVYMHDIGKINSSFQFVKM
                     QNKNFKKISLRYTKHSFISSMLYFDYYFEKLKKYDFEEKKILSLFLMINTYTISKHHG
                     VLKSFKEFLFLFKDEFINIKENFKDLFKNNYNLELKSVPKTIEKMVRGVEAYLDDSHI
                     KDNLAIDLYIYSKLMFSIVTSCDFYATSEYNSGNSIDDFGTIDNYNKYYDVLKKSEIY
                     KGMKKYREYLNNNSECPYKENDINRLRTEMSIEAEDAITHNTRKRIFYLEAPTGSGKT
                     VTSINLALKSIELNQSLKKIFYIFPFNTLVEQTKDVFINEIFNSVKDIQKDVVVINSI
                     TPIQTEDKEEDNKNVEYCVTKQEIDYDKSLLNRQFLHYPIVLTTNIGFFNYLFGVSRE
                     ECFPLIHMINSVIILDEIQNYKNEIWKEIILFLEKYADILNIKFIIMSATLPRLNKLG
                     CNDDNFAYLVKNREKFFKNHLFKDRVNISFEMLNSEINDIEEISEKVIEEAEIYNKIL
                     IEFIKKSSALKFYKNIKEKLKYKLEDVFLLTGDDNKLERKKIINKIKDMNSVILVATQ
                     VVEAGVDIDMDLGFKDISTIDSDEQFLGRINRSCKKLNSKVYFFNLDDASKIYKKDVR
                     KERHLTLNEESNRKIILDKDFEKYYDKIIARIEENKHKHNDKNIEVFIKDIVGNLNFT
                     EVKKWMALIPDLKEYTIFLNTIVKDESGNMIVGSDVWYEYISLLKNDNVEYSEKRVKM
                     SEIMEKLDYFTYKVQKFDNSFNDLVGDIFYIDDGSKYFTEGKFDRSKFNQNEFL"
BASE COUNT         4710 a          960 c         1656 g         3920 t
ORIGIN      
        1 ttattgcaca aaaatagata taatttaagt aatataccaa aaaaaaggaa tatatttaca
       61 taatatgata aaataaattc aaagagttaa attgtaggta aatagtattt taattattta
      121 ttaaaatata gattatgtat atgccaaatt taaaaagaga cataaagaca ttatacaaat
      181 atattattgg ggtgatattt tgtaagaaaa gaagtgatgc agtaaattaa ttgagtaatt
      241 taaaaatgat ttaatgataa tgttgatgaa tgtttaaaaa gaaattttag aaaaacgtat
      301 attgctgttt gtgttggagg tgatgaattt gtagaattta tagggtataa atgattaaac
      361 tatttaagat aaatacatat cactgtaaaa tattgataat cattttttat aagagattga
      421 ttatttgcaa attatatata ttacaaatga ggtaacaata atgaagaaaa aatatgattt
      481 tttaatttat tctgctattt ggctagttat tattattctt tttatatttt ctctatatca
      541 tagtatagag catattaaaa ttataagtca cactggtact attcgcagtg aaactcaaaa
      601 ggttgttaag caagaattga ataatgaaag aaatgatgac ttgataaaac gactggataa
      661 tattctgata aagctaagaa caggtaatgg agaaaatgga tttcaaagat gtgataataa
      721 agaatttcaa caaaaactaa atcaaatgga ttctatgtgg gaatctatga agaaagaaat
      781 tataaaagtt agaaatggtg cttcaggtga taaattatat aaattgagtg aagagtattc
      841 tgtgttgtct aatcaaattg tttttatatc tgaaaaacac tctaatgcaa aattgtattc
      901 ttttgctact gcactattta tttatcttat attctcaaca ataagtcttt taatttggga
      961 atattataat aaaaaaaggt ttaaaagaat attttatact gataatttaa caaaaataaa
     1021 aaatcaggtt gcatttgaaa acagagcgat tgaaattttg cacaatgcat ctaacaaaga
     1081 gtatgtactg ctaaatattg atattgacaa ttttaaatat attaatgata cacatggata
     1141 tgaatatgga gataaagttc taataatagt tgcaactgtt ctttctaaaa cttttaatat
     1201 aaaagaaact tgtgcaagaa tttgttcaga taattttgta atacttgcaa aatatagaga
     1261 ttctctttta gaggatattc gggaaatgct aactaatgct ataatctctg aattagatat
     1321 gaatgtaact caaactatat cttattgtat aggagcttac ttagttgaaa ttgataattt
     1381 aggttataaa tctatcaatt ctatgatgga taaggcaaat attgcacaca aggtaagtaa
     1441 aacaagagga atttcctcca cagtatggta taatgaaaat ctgttaaaac agttacaaat
     1501 ggaaaatagt atttataatt atatgtataa agcgatagaa aatgaagaat ttcatatgta
     1561 tttgcaacct aaatttcaaa tatcttcttt aaatgttgtg agcgcagaag ctcttgtaag
     1621 atggttttct ccagaacttg gttttttatc accagatgaa tttattccat tgtttgaaaa
     1681 aagtggattt attattgaat tggactttta tatgttaaaa aaagcgtgtt catttgtaaa
     1741 aaaaacgttt atgaaaaaaa atcaatatac atatccgatt gcagttaatt tttcaagggt
     1801 tactatttac caaaatagtt tttatcaaag gtttttagat actgttaaag aatatgaaat
     1861 tccttttaag tatatagaga tagaggtaac tgaaagtgct tttaatgaaa tatctcaacc
     1921 agttatttca attttagagg aacttaaaaa gttaggattt ttaatttcta tggatgattt
     1981 tggatcagga tactcttcat taagtctttt atgttctcta tctattaatg gattgaagtt
     2041 agataaaagc ttacttaaag aaacatttaa ccgggagaaa gtatatagta ttattcagtg
     2101 tattattgaa atgtcacatc gtataggcat gtctgttgta tgtgaaggta ttgaaactaa
     2161 aaaagacttg gaatttttaa atacagttaa atgtgatgta ggacaagggt tttatttttc
     2221 taaaccgata gaagaaaaag aattttttaa caaatatgta actaaaaaat aatataatta
     2281 tttttatcag tctatacatt gagtcatttt atatctcgtt aaatgactca atgcactgtt
     2341 atgtatttta aaatataaat tatttaaaac gttttttata cttatataga aattcttcta
     2401 tattttttga ttgttctctt atttcaactg cttctatatc tagtaaataa agtatagaat
     2461 catttgtaag ttcaacatat tcaccatctt ctgaaccgag tgcatgtctc tcaattttta
     2521 atcctttttc ttcataatat ttttttgttt ctatatataa tgattctcgc ttttgtatgt
     2581 agtctttcac ttcattattt ggaaagttat atgaatccac taatgtttcg ccatttataa
     2641 aagttttctc ggtagcaact ttttcatccc aacaatggat ttcttcccaa gtaaaaaata
     2701 tctcagttcc atctggaatg aatgaaaacc tggtaataat atataaataa ttattgaaat
     2761 ttacttcata atattcagga atagcttcaa attccatata gtaatttgta taattaaagt
     2821 tactactatc tttttttaca aatggtattt tctcaataaa tttatatatt gattgttcat
     2881 ttttaaaaat tcctatatca tagcttcttt caaattgatt aatttttaat aaatatgaca
     2941 tatattctcc ttatttgttg aaatataatt tttataactc tactaatttt aaacattttt
     3001 aaataaatat ttgtatatac cttatataat attatagtaa ttagttttaa atcaatagaa
     3061 tagtatgtat ttatctaatg cggcaatata atttaaagct ggtgttgtag aaaaactaag
     3121 agtgaatata gcaaagagaa gcaatagtat tttaaacttt gtctctctta ttttttgaaa
     3181 actaacatta aaacataagg tataaataat attatcatat aaatgaaaac aaatttgaat
     3241 ttttctctgg tcgatgtata tagtaagttt gttgatatga gaaaaattat tattgataat
     3301 atacatgcat agaaaggaaa tggtatataa tattttctta agtttctttt ttaggcattt
     3361 ctattggcat tttaggctat agtactaaat acattaacca acaaaataat gaaataaata
     3421 tatatataga aaacttaatt ttcgtgggaa gaaaaaaagc aataatagca agtattatta
     3481 gagaataacg aataatacga gtaacatttc tttctttttc tatgatagtt aaatcccagt
     3541 gattttttat aaattcaagg tctttttctt gactctgatt catttaatca tctcctgtta
     3601 atagtataat tttaaagcta ttactttgtt ttattttaac atatttatac actaaagtga
     3661 attttatgat tagatttatt taattatttc tgaattaaaa cttattagaa ttctctattt
     3721 tgataatttc aaactctatt gattgtatga taattgaaac tataggatat gtaggataac
     3781 aagtttcaaa gtagttaaaa taaaaaagat aatgatatta ttaatttaga ataaatttta
     3841 tatattatat ttaattattt acttgtttta agttttatta acttaaaaaa tgtaagaaat
     3901 ttattatcaa taatctaaat atagaaatta aaaatgataa agagattgta attatatttt
     3961 tttgaaactt tgtgatttta tttgtcgaat aaaaaataaa aaattttaaa ggaaaaagtt
     4021 aaaaaataaa gaattttata actataaatt atattttaga gtttattgtt attaaataga
     4081 tgaaaaaatc ctaatatgga ttatcagaaa atgaggaggt tgttaattga taaaatgtct
     4141 tgaaatgtct gtaaaagtgt atcttcttga aaatatttct ttagataata catcaaaaga
     4201 aatacgtaaa ttaatagatg atacattaat tatagaagaa aaatataagg aatttcataa
     4261 taaagctgga tttaagaatt attcttttaa tttattatac ccattagaaa aagataaggt
     4321 ttataagaag gattgtatat atacttttat tattagaact gtagatagtg aattaggaat
     4381 gtattttatg aataatttaa aaaatcagca ttctgataaa ataaaggtta ttacaatgag
     4441 taaaaaagtg atccctaaaa aacatataga aagtatttac tctgtcactc caataatagc
     4501 taaatttgag gatgggtatt ggagaagtaa taaaacagta gaagatttag aaaaaagaat
     4561 cagagaaaat ttaataaaaa aatataatga atttactaac agtaaaatag atgaagattt
     4621 tgaattattt caagttatta aatttataaa taggaaacct atttctacta aatataagaa
     4681 tatatcttta cttggtgata aaataagttt taaagtatca gaaaatgata tggctcagga
     4741 aattgcaaat tttgcacttg gagttggagt cggagaaatt aattctcgtg gatttggttt
     4801 tgtaaactat agatggttat aggaggtgaa aacttgttaa aggattgttt agagattttt
     4861 gaaaaagaat acaaatcttt aggaaataga ttgataactg atagctatgt cctatcctat
     4921 ggaacatata tattggtgaa aaatttttcg aatatagata tattagaagt ttctaaaaat
     4981 aatgtggata gtacagaaga tatataccat aaattttgtg aactggatta tttatcaaaa
     5041 ctggtgtcta tagataaatc agttgattcc aaaaaaataa tacatagtaa taattatctt
     5101 tcattttttg taaaaaagga aaacttaaga cctgataaaa ataataatgt aaaacttaca
     5161 gaagaaatta ttgataatta ttatgatgct ttgttaaacc caagaattaa atataaatct
     5221 gataaaagaa aaaaagaaat ctatgattca atagaaaata aatatggtaa agtggatgaa
     5281 aataaattaa ataaaataag actatggata aaaacaaata tatttgattt agttgaatct
     5341 gttaaaaaag ataaaaatta tttaaaaata ttttttgatg aaaaaataga gttatacaat
     5401 gatgaaaacc aaaaatattt atatccaaat atatataata aaaatgattt caatgtaatt
     5461 attgatggta aaaactatgg gttaccaaac gataatatgc aattaaatga aaaaaagcca
     5521 tttttgaaat taaaaacaag aattagaaat tcaactcctt atttgattag tgatgatgaa
     5581 gttttgttac agaaagcttt ttttgattat ctaacgaata aagcagcgaa tggagaagaa
     5641 gtaatatata taagtagcga aaagggaatt catagaaaaa ctgatatacc aaatgaattt
     5701 agtggttata tactgagaat aaaaaagggt aaagaattag aaatacatga ttttgatact
     5761 gtggtaaatt taaacactaa aataaaaaga ataaatgtag aatgtataat accaatagaa
     5821 tatgaaagac ataatttaaa accaaattta gagataggat atattaataa tcttataaaa
     5881 ttggaaaaaa cgattgatac tgtattcttt aataaattta tgtgcaatag ttattttaaa
     5941 gaaattaaaa atataaactt aagtgattta gttctaaggg atatacttat tagacataga
     6001 gaaggtttat atacctggtt ttataaaggg agagatagta tagtaaagca aactttttca
     6061 aactattcta tgggtataat tgaaaattct ataaaaaaca ataactacat aagagcaaag
     6121 gagcaatata atttaagaat agctataata aattattttc atggaggaag taatatggca
     6181 gataaaataa aagttattag tgaatcttta tttgaaaaaa taaattctat aaaaacgaaa
     6241 tgtatacaaa gtgatgaaga atattatttt gcagttgggc aattagcatc gtattttata
     6301 tctaaaaata agtcaaaaaa taaaaatcat tctctgatta atcctatact aaatgcacaa
     6361 aatagaaaaa aaatcatgtt agagatagaa aaattattta aaaaatataa ttatgatata
     6421 agttatgaca acaataggcg atttaagaat ctatattcta tgattgtagg atatgaggta
     6481 gaagaaaaaa aagttaatag agatttatta attggtggat atctatatta caatttaata
     6541 tacacaaaaa gtgaggagaa tgaaaatgaa tagaagagta tatggagtat taggtataaa
     6601 atctataatg tcaaactgga atgctgattt ttctggatat ccaaagacga catcaacagg
     6661 agaagttttt gggagtgata aagcacttaa gtttcccatg aaaaaaatgt gggaaaatga
     6721 aggtgaaaaa gttttatatg ttaaatcatt aaaaatagat gaatctaaga aaggagaaat
     6781 aaaaattaga ccaaaatcat taaaagagag atatgaagaa ttgtttgata tagaggactt
     6841 aaaactagaa aagaatcaac aaaaagtact ggaaaatcta tttaaagcta ttgatgttaa
     6901 aaattttggt gcaacatttg cagaagaagg aaataatata ggaattatgg gcgcagtaca
     6961 aattggacaa ggatttaata agtatgaaga tattaatgtt gaagaacaac aaatattatc
     7021 cccatttagg gatccaaaag caaaagataa gaaaaagtca acaaatgaag aagaaaaaga
     7081 agatgcaaat tcaactacat taggaaaaaa aatagtaagt gatgaggctc attactttta
     7141 tccattttca ataaatccaa aagcttatga agtttttgtt aatatgggtg tgactgatgg
     7201 atatactaaa aatgattata aaaaatttaa agaagctgct ttagtaagtg caactgcatt
     7261 caatacaaat tcaaagcttg gttgcgaaaa tgaatttgca ttatttgttg aaactgatga
     7321 agagttatac ctaccagatt tggcacaata tataactttt gaaaagagag aaaaatcaaa
     7381 agatttaatt tgtataaata taattcatct tatagcagaa ttaaaagatg atataaagag
     7441 cgttgagata tattataacc catataaaat agaaataaag ccttttaaag atgaaatatt
     7501 acctaagata gataaagtta aatttatgaa catatttaca agaaaagagg tataatatgg
     7561 aggttttgaa atttaactta agtggaaaaa ctgctttttt taaaaaacca gatgtaaata
     7621 gcataattta ttttacgtat ggaaacatac ataaaattgc acttatgggt attttaggag
     7681 cttgtttagg acttggtgga tataatcaac agagtaagga agatgagtat ccagaattct
     7741 actctttact taaggatata aaaatgtcaa tagttccaaa gcatgaattt ggatatataa
     7801 ataaaaaagt ccatatattt aataattctg taggatatgc atcaaaagaa gaaggtggaa
     7861 atttaataat aaaagaacag tggcttgaaa atgttagttg ggatatatat atacaattag
     7921 aagattcaac tacaagtaga gagcttaaaa gaagattttt atctaataaa tttgtatata
     7981 taccttactt aggcaaaaac gatcatatag ctaatataag tgaaatagag ataattactg
     8041 atttttacaa aacagaagat attaacagag tggacagttt ctttataaga gaggattttg
     8101 agtttgaaaa atcttatttt gatgataatg atgattataa agagccattt aaatatcagg
     8161 aaaaacttcc ttatagactg caccgaatga ctaatcaata tattttggaa actatcatat
     8221 ttactaatat gtcagttaaa ttgattaata aaaaagaggt atatcaagta aagggtttaa
     8281 atttaatctt tatttaaatt tataggttta ggagaattat atgtacttta aaaaagttga
     8341 gttgtttgat ataagtaata atataaataa ttgcaaaaat atttatgctc atataaataa
     8401 agacaggaag ccagaaactt taaaagaaca ttcagactta tcatactcat atcttatgaa
     8461 attatgtgta aaaaaagatt taagctcttg cctagagaga atagagaata aattttttaa
     8521 aaaagattct tttttaagta agaattttta tagagagatt atcttgaata tggtttatat
     8581 gcatgatata ggtaagatta attcttcatt tcagtttgta aaaatgcaaa ataaaaattt
     8641 taaaaaaatt tcacttagat atactaaaca ttcttttatc tcttcaatgc tatattttga
     8701 ttactatttt gaaaagctta aaaaatatga ttttgaagaa aaaaaaatat tgagtttatt
     8761 tttgatgata aatacatata caatatcaaa acatcatggg gttctgaagt catttaaaga
     8821 atttttattt ttatttaaag atgagtttat aaacattaaa gaaaatttta aagatttatt
     8881 taaaaacaat tataatttag agcttaaatc agttcccaaa actatagaaa aaatggtaag
     8941 aggtgtagaa gcgtatttag atgatagcca tataaaggat aacttagcta tagacttata
     9001 tatatattca aaattgatgt tttcaattgt tacatcttgt gatttttatg ctacatcaga
     9061 atataatagt ggtaattcta tagatgattt tgggacaatt gataattata ataagtatta
     9121 tgatgtatta aaaaaatctg aaatatataa ggggatgaaa aaatataggg aatacttaaa
     9181 taacaatagt gaatgcccat ataaagagaa tgatataaac agattgagaa cagaaatgag
     9241 tatagaagct gaagatgcaa ttacacataa tacgagaaaa agaatatttt atttagaagc
     9301 accaactgga agtggaaaaa ctgttacatc tataaatcta gcactgaaat ctatagagtt
     9361 aaatcaatct ttaaaaaaga tattttatat atttccattt aacacattag tagagcaaac
     9421 aaaagatgta tttataaatg aaatatttaa ttctgttaaa gacatacaaa aagatgttgt
     9481 agttataaat tcaataacac caatacaaac agaagataaa gaagaagata ataaaaatgt
     9541 tgaatattgt gttactaaac aagaaattga ttatgataaa tcattgttaa atagacaatt
     9601 tttacactat cctatagtac ttacaactaa tataggtttt tttaactatt tgtttggtgt
     9661 atcaagagaa gaatgttttc cattgataca tatgataaat agtgtaatta tattagatga
     9721 aatacaaaat tataaaaatg agatatggaa agaaattatt ttgtttttag aaaaatatgc
     9781 agatatatta aatataaaat ttattataat gtctgcaact ttacctagat taaataaatt
     9841 aggatgtaat gatgataatt ttgcatatct agtaaaaaac agagaaaaat tttttaaaaa
     9901 tcaccttttt aaagatagag taaacataag ttttgagatg ttaaattcag aaattaatga
     9961 tattgaagaa atatcagaaa aggttataga agaagcagaa atatataata agatactgat
    10021 agagtttata aagaaatcat cagcactaaa attttataaa aatattaaag aaaaattgaa
    10081 gtataagtta gaagatgttt ttttattaac aggtgatgat aataaattag aaagaaaaaa
    10141 aataataaat aaaattaaag atatgaactc tgtaattttg gttgctacac aagttgtaga
    10201 ggctggagta gatatagata tggatttggg atttaaagat atatctacaa ttgattctga
    10261 tgaacaattt ttgggaagaa taaatagatc ttgtaaaaaa ttaaattcaa aagtatactt
    10321 ttttaattta gatgatgcaa gtaagatata taagaaagat gttagaaaag agagacattt
    10381 aactcttaat gaggaaagta atagaaaaat tattttagat aaagactttg aaaagtatta
    10441 tgataaaata atagccagaa tagaagaaaa taagcataaa cataatgaca agaatataga
    10501 ggtttttata aaagatattg tgggaaattt gaattttaca gaggtaaaaa aatggatggc
    10561 tttaatacca gatttaaaag agtatacaat ctttttaaat actatagtta aagatgaatc
    10621 tggaaatatg attgtaggaa gtgatgtatg gtatgaatat atatcactac taaaaaatga
    10681 taatgttgag tattctgaaa agagggtgaa aatgtctgaa attatggaaa aactagatta
    10741 ttttacgtat aaagttcaga aatttgataa ttcatttaat gatttagttg gagatatttt
    10801 ttatattgat gatggaagta agtattttac agagggaaag tttgatagaa gtaaatttaa
    10861 tcaaaatgaa tttctataat gtatttagat tatatagaga atatttatcc caagttaatt
    10921 aattagaatt agtttaaaac tattgaaata tagatatttt atagcttata tattttttct
    10981 aggattagct gggataaaaa atctgtatat tagtgttttc agtgtattta aggggattat
    11041 atttttaaga agtactgatt ttaaagtggt taaacagtaa catgagatgt atttaaattt
    11101 ttactacata attaaatcgt agtcaatacc gtacaggtta aacagtaaca tgagatgtat
    11161 ttaaatctct gaatagccaa tcaactttgc tgcattactc ttgttaaaca gtaacatgag
    11221 atgtatttaa atgataatag ttatat
//
LOCUS       DAYAOX010000117        11206 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.117, whole genome shotgun sequence.
ACCESSION   DAYAOX010000117 DAYAOX010000000
VERSION     DAYAOX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.117"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            124..1137
                     /locus_tag="ACGI57_002410"
     CDS             124..1137
                     /locus_tag="ACGI57_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFG9717485.1"
                     /translation="MNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYIFISDKF
                     DDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNIRLINLS
                     LSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFTLDGDDS
                     FWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEKPGITIS
                     DLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCLDIEENN
                     EKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLTELVERY
                     KNL"
     gene            1270..2568
                     /locus_tag="ACGI57_002411"
     CDS             1270..2568
                     /locus_tag="ACGI57_002411"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFG9717486.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLSHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(2682..2927)
                     /locus_tag="ACGI57_002412"
     CDS             complement(2682..2927)
                     /locus_tag="ACGI57_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HFG9717487.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3075..3941
                     /locus_tag="ACGI57_002413"
     CDS             3075..3941
                     /locus_tag="ACGI57_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HFG9717488.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(3945..4481)
                     /locus_tag="ACGI57_002414"
     CDS             complement(3945..4481)
                     /locus_tag="ACGI57_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HFG9717489.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(4672..4875)
                     /locus_tag="ACGI57_002415"
     CDS             complement(4672..4875)
                     /locus_tag="ACGI57_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFG9717490.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5166..5930
                     /locus_tag="ACGI57_002416"
     CDS             5166..5930
                     /locus_tag="ACGI57_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9717491.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6319..7632
                     /gene="prdC"
                     /locus_tag="ACGI57_002417"
     CDS             6319..7632
                     /gene="prdC"
                     /locus_tag="ACGI57_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HFG9717492.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8037..8122
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            8421..8549
                     /locus_tag="ACGI57_002418"
     CDS             8421..8549
                     /locus_tag="ACGI57_002418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717493.1"
                     /translation="MDNQQVSFEKKKLQRLERNLVPTSVGKWGVPNLWLGNDIVCA"
     gene            8704..10878
                     /locus_tag="ACGI57_002419"
     CDS             8704..10878
                     /locus_tag="ACGI57_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HFG9717494.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQTPDVEKPSDGQ
                     MPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEED
                     VKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTL
                     TFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQE
                     NQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWIILGQMDEQERL
                     KQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAGREELKPNDTGK
                     PSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLRLPDGVTAYDGDGNIIGTGEVE
                     ISTQQKFKLKSVVKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQN
                     LLSYTIEKKNGQEVNFNIGLPTDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPP
                     MDDTIINPPVPPTEDAVLNPPVPPTEDAVLNPPVPPTEDTVLNPPVPSVSDTVEKTPE
                     LSRDDTIIKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
BASE COUNT         4529 a         1243 c         1947 g         3487 t
ORIGIN      
        1 attttatgtt tttagcaaga aatatagcat ttaaaagata aattgtgcaa cttaaatcaa
       61 agtttaagat gaactattaa catagatata ttaaaaactt ttgtaatgga gaatatatgt
      121 gatatgaata ataaagttaa ggttgcagta ttagatacag gaatagacaa agaacatgat
      181 tacttaaaag ataatttagt tggaggaatt gcttttgaat gtatgcatga ttatatcttt
      241 atatcagata aatttgatga tgaagatggt catggaactg cttgtgcatc aataataaag
      301 aaagagtatg aagatgtaga gttatttgta ataaaaattt tgagaaaaga tggtattaca
      361 aatataaaag ttcttgaaga agcattaaga taccttttag acactaacat aagattaatt
      421 aatttaagtt tatcagtcat aggagtagaa agtgttaagg gcttatttga agtttgctat
      481 gaattgttta gaaaaggaaa aataatagta tgttcactcg ctaatgattt tgacttaagt
      541 tatccagcta tgtttaacaa tgttataggt gtaagaggat ttactttaga tggagatgat
      601 tcattttggt acaacaaaaa atatgatgtt caatgtgtta tggatagcaa ttcatatata
      661 agttgtgata taaataattc ctatagatta ccaccaaaat gtaatagtta tttatcagca
      721 aagcttactg gtaaaatagc taagatatta tcagaaaaac caggcataac aatatctgat
      781 ttaaatgata agcttgaatc attagcaact agaaatcatt gggatagttg tgattttgat
      841 gagtgtagta gaattgtaaa ctttaaattg gatttgtatg acaaagaaaa tgctcttttt
      901 atgaaagttg ctgacgttgt tagggattgc ttagatattg aagagaataa tgaaaaactc
      961 tttaagggta gcctttttaa taaggagata ggcttggttt atgataattg ttttaatctg
     1021 ttgaaaaagc tagagcatag atttgacata aagtttaatt atatggacat ctcaaaatat
     1081 gacttagttt caatatatac actgactgaa ttagtggaaa gatataaaaa cttataaata
     1141 aagatttgta gctaaattta atgtagaagg tattaaatta tatgtattct ttgtcggtag
     1201 attaaaagta aaaaatatac tataattaga tagcataaga gaaaatgcca atacaggggg
     1261 agttagataa tgaaatatga agaagcatta gagtacatat cacaaactaa taaattcggt
     1321 ataagattag gattagaaaa tatcggaaag ttattagaat tattaggaaa tccacaagaa
     1381 actttgaata taatacatgt agctggaact aatggaaaag gttctgtgtg ttcttttgtt
     1441 tctaatatac taagagagtg tggatataaa gttggtttat atacatctcc atatttagaa
     1501 acttttactg agcgtataag agtaaatgga caaaacatac cacaggatga tgttgctaga
     1561 ataattgagt taataaaaga aaaaatagag attatggtaa aagaaggcta tgcatatccg
     1621 acagaatttg aagtggttac tgcaatggca ttttattact attcagaaca aaaggtagac
     1681 tttgtggcat tagaagttgg tttaggtggc agatatgatg ctacaaatat aataacaaaa
     1741 tcacttgtaa gtgtgatagt atctataagt ttggaccata caggtatact tggagataca
     1801 atagaaaaaa tagcgtacga aaaggctgga ataataaagg aaaatggagt agtactagta
     1861 tatgaccaga ctgatgaagc aaaggatgtt ataaagtcag tttgtaaaga gaaaaaagct
     1921 aaatatatag aagttgattt tgatgatata aacataaaaa aatcagatat aaattctcaa
     1981 atatacgatt gtactgtaat gaaagaaact tatagagacc ttgaaataaa attgataggc
     2041 gaacatcaaa taaacaattc tatcttggca attagtgtaa taaaatatct aaaagacata
     2101 aataaattag caaatataaa tgaagaatct ataagaaaag gacttataaa tactaaatgg
     2161 ccaggaagaa tagaaaagat aaaggaaaat ccaattttta taatagatgg tgcacataat
     2221 gaagatggag caaaatcttt ggcaaaagca cttgataaga attttaaagg tagaaaatta
     2281 accttattaa taggtatgtt agaagataaa gatattgata gtgttttaga gatattgttg
     2341 tcacatttta ataaggtaat aacaacaaca ccaaataacc caagggccat aaattcagat
     2401 atattaagag aaaaagtatt aaaatatgta gatgatgtaa catcaaagca tgagatagag
     2461 gatgcagtaa attatacatt agaaacatca agtgaggatg atattataat aagtgcaggt
     2521 tcattatata tgattggtac agtgagaact ttagtaaaga aattataatt ttataaaata
     2581 agtatacaaa taaaaatata aaaaagagtt gtctaaaaaa tggacaactc tttttatata
     2641 aaataagttt ttaataattt tactgatgtt tatataagta attatataga ttgacgtata
     2701 gctttagata attggtctgg gcaagaagta ccttttccac cacagtcaat accatttaat
     2761 ttatcagcaa cttcaagagc attttggcca attattaagc ttctaagtcc tgataaatta
     2821 ccattacatc ctcctataaa ctcagcatca acaatcacat tattttcatc aacttcaaaa
     2881 atcatttctc tacaacatac accacttgga ttaaatgtta ctttcattaa aaagacctcc
     2941 attaaatatt atttcttttt ttaacattaa taataatacc aaatatatgc gtaataatgc
     3001 aactataaag cataatacaa gctaatgaaa taatatatta ttgtagtata tgtattgtag
     3061 agaggagggg ttatatgaaa atctatattt ctaactactt atcaaagagt attagcatag
     3121 ttgattattc tacacttgaa ctagaaaaag aaatagtatt agaagataat atatatccac
     3181 accatttttg catagaaaaa gaaaaaaatt taatatacat acctagttca agtaatggga
     3241 tactctatgt cttagattta agtaatgata aaataattga tactgtctct ataggaggaa
     3301 gtcttagtca agttgtactt agcgataatg aattgtttgt ggcaaatgaa gattctaata
     3361 gtatatatgt actagataaa aacacattaa atccaatagg aattattgga gttgataata
     3421 tgccacatgg atttgacttt gatagagaaa gcaaaaagtt atatgtaccg tgtataaact
     3481 cgatagtatg tatagataca attaataaaa atatacaaaa gaaaataaat atggacttta
     3541 gagcttggca tatagaactt gataaacaaa agaaagaaat gtatatatca acacttgatg
     3601 gaaagcttat aataatagat gaagtaagca tggatataaa aaaggtatta tatgaatttt
     3661 tattaccagt agaaataaga tttaattata gtggcaaaaa agtttatgtt gctgatttgg
     3721 gttataataa tgtaagaatt ttagattata caacgagtag atacattgga aatattgaaa
     3781 taaatgggat tccccaagga ttagaaatat ctaaagatga aaagttacta tttgtatcag
     3841 atacacaaga aaattcagta aaagtttatg aaactgcaaa taacaaatta ataaaagaaa
     3901 tcaaggtagg aaaagagcct acaactataa tttgtttgta ggctttaatg tatgtctctt
     3961 ataagtaaat cgataacttc ggcatacata taagatgcat tatttttcca gttattgcaa
     4021 ataagttttg cttgtttctc tgatgaaaca tttagattta aatctattaa gttagactga
     4081 ttttctatga ctctaaggtt gacaataaat tcattatcac tttgttttac aaaactagac
     4141 tttacttgag tatctgctag tagattttct ttgttagaat ctatatactt gtctattttt
     4201 tcagtaacac tagtaggtat tcgattaaag aagtattcta gactttctac gcctctttga
     4261 gtaagagcat aatattctct gtcagagtct ttatatgtag tcaaaaactt agattccata
     4321 agctgagata aaagctgttg taatgaaaaa taattcatca tctcagtctc taatactact
     4381 tgagttattt gagaattagt taaatccatt ttaatttttt ctaatatata taaaattaaa
     4441 agtttgtgat aggctaattc ttcagacgag ttttcaaaca taaatatcat cctccgtcaa
     4501 actatttcta tatataatat attaaactaa tttgtacaaa attaaaagaa caatttgtat
     4561 tgtattatta atctttaata attcatataa aaacaatttt ataaataaat atagaaaaac
     4621 agggctgcta attagcagcc ctgttgatac ccaggtgtca aaatttacta tttattttcc
     4681 agccatttgt ctttcagcca tttcaactaa tttcttagtc atatatccac caacatatcc
     4741 attttctcta gctgttaagt tacctttgtc tatgttttca tagttagata aacctatttc
     4801 gttagctatt tctaatttca tttggtttaa agcagctttt gcttctggaa ctactgttct
     4861 gtttgaattt gacatagtaa atttcctcct caaataatta gtttatgatg tctaacaaca
     4921 cttttattta tgtgatgtta atactaattt ctccaaaata aaaataatat atacagaaaa
     4981 ataaaggtaa aaatgtaaaa aatataaaaa aattaaataa atgagtattt tagagagttt
     5041 aattaggtca atataggttt tattttttta gttattgtct tataaattaa atattacagt
     5101 acttttttga gctgaaaaaa aataaatttt aaatatagaa gaaattattt caaaggggga
     5161 atagtatgaa aagagaagtt aaaaggacta aagctttgat atatagtgtt ttattttttt
     5221 cattattatt agtgttaata tttgtttttg tttatggaat aaaatacata tgttctaaat
     5281 ctgacaattc aaaaatgcct atatataaag ttgatacaaa tgagaaaaaa atagctttga
     5341 gttttgatgt agcatgggga actgataata tggatgatat tttaaaaatt ttagataaac
     5401 ataatgtaaa agctaccttc tttttagttg gtagttgggt ggatgataat gaagaaattg
     5461 taaaagctat agatgaaaaa ggacatgaaa ttggaaatca ttcaaatact catgccaatt
     5521 tgaaggaaat atcaaaagaa gacataacaa aagagataga aactacatca gaaaaaatat
     5581 ataatataac aggaaaaagg actaatttat ttagacctcc ttttggggac gtaaacaata
     5641 aagctatgga catttgtagt gatttaggtt ataaagtaat taagtgggat gtagattcta
     5701 tagactggaa ggagcttgga ccaaatcatg taattgaaaa agtaataaaa gaatctcaac
     5761 ctggttcaat agttttattc catgctaata taaatgatgt agataactat ttggatacta
     5821 taataagtcg actaaaaaaa gatggataca gtctagtcaa aatatctgat ttgttgtata
     5881 aagataatta tgtagttgat tccaatggag tgcaaaaaac taagaattaa taagtaataa
     5941 atgtatattt taagtaaagt tgtgataaat tatatagtat atgttagaaa agtgtcgata
     6001 aaaagacggt gtctattttt tgacactttt tatacatgta tgcaatatca ataattatag
     6061 attaaattga ttaaaaaagt gtctaataat tgactttttt ttgatgggtt taaaaattta
     6121 gaagaattaa tgaatgctgt aagacagtat ttccaaaggg tataagtttg tttaaaagtt
     6181 ggcatagaaa ttgctttgtt aataagcgat atgttctata gaaataaaca atatgttaag
     6241 accttataat agaaaaatag cattaacatt atttgaacag cttataaaaa aattaatata
     6301 cttaaggggg cgtactagtt gaacaaacta tattctattt tattaaaaca acacgttggt
     6361 ggtccagata agccagtagt aagtgttgga gatgtagtaa aaaaaggaac tctaatcgca
     6421 gaaccaactg gattaggggc aaatatttat gccagtgtta gtggaaaaat aagtgaaata
     6481 aatgaccaag cgatagtaat tgaagctgat gaagtacaag aagatacttt tgaaccatta
     6541 aaaggagaag gtatacttga cttaattaaa gaagctggag tagtaggaat gggtggagca
     6601 ggattcccta ctcatgtaaa attaaacata gatttaaatg gtggaacaat attagcaaat
     6661 gcagctgaat gtgaaccttt attagctcat aatataaaag aaatagaaga aagaccagaa
     6721 atagtttatc aaggtataaa atatgctatg gaagttacta atgctggaaa aggtatgcta
     6781 gcaataaaaa gtaagcatcc aaaagcaata gaagctttca aaaaagtaat aaagccagga
     6841 gacaacatag aagttgcaga actagttgat atgtacccaa tgggagaaga gagagcaata
     6901 gtaagagacg ttctaggaaa attacttgag ccaactcaat taccttcaga ggctaatgca
     6961 gtagtaataa acgtagaaac actaactaga atagtagagg ctgttgagca aaaaagacca
     7021 gttatatcta aaaatataac tgtagttggt caattaaaca gtggaaaaga atctatagtg
     7081 tttgaagatg tgccaattgg aacaactgtc ggagaattaa ttgaaagagc tggtggaata
     7141 aaaggtgaat atggtgaaat aatcctagga ggacctttca ctggtaaagc tactacttta
     7201 gatgctccaa taactaaaac tagtggtggt ataatagtta ctatgccatt tgttaatgaa
     7261 aagagaaaaa tgggtctttt agtttgtgca tgtggaccta atgaagaaag aatgagagat
     7321 atagcaacta aaatgggagt tactgatata gtatcagttc aaaaatgtaa acaagctcaa
     7381 gaaattaaag gtgcattaaa atgtgaaaat ccaggacact gtccaggaca agctcaaaaa
     7441 tgtattgaat ttaagaaagc tggagcagaa gttatattaa taggaaactg tactgactgt
     7501 agtaacacag taatgggttc tgcaccaaaa ttaaaattag gtgtatatca tattactgac
     7561 catgtaatga gaacagttaa tcacccatta ataagaagaa taaaaggtga cgtaagatta
     7621 aaaataaaat aatagataaa tagaagttag acatctttag aagtaaaact taaaagttac
     7681 ttctaaggat gttttttaat gtattttgag ggcaatatat atattgtaaa ataaatggaa
     7741 aaaatagaca aaatatgcta gatattgtat aatgataata atagctatta caattaatat
     7801 tggacaaaaa taatgatact caagtatgtg gtttgtatta atatgcatat tatattaagt
     7861 caactatccg atagagtaaa aggtttttgt gatatttgaa gatactctat agtatctttt
     7921 ttattataaa aactaatttt tgtcttttat tagataagta tctaggatac taatttatat
     7981 tttattgagt tgttaaaaaa gtgcacttaa aaagttaaaa aagaaaaatt aaaaaaccta
     8041 tttatagaaa ctgtgaagta tatcttaaac ctgggcactt aaaagatata tggagttagt
     8101 agtgcaacct gctataaata taaataggag taacttttaa ttgtcaaaaa tatataaaat
     8161 aaatacataa ataagtaatt aatagaagat agatataaat atatttttga aatgctccct
     8221 gcattcataa cgagtgtaga gtggcagata tctgcccaaa actcctttaa ttgctggaag
     8281 ttcctaaagc taactaaacc acaacataat catgaaataa ggataagtgt gatagagtgt
     8341 gcatttaata agcacttgag gttacgaaag tagaaaaaat tagttagata gcataaggtt
     8401 aaatcctaag tgccttgaaa atggataatc agcaggtaag cttcgaaaag aagaaacttc
     8461 aacgactaga gagaaatctc gtacctacaa gtgtggggaa atggggagta cctaatttat
     8521 ggttaggtaa tgatatagtc tgtgcttaat agaaatatta agaagttcat aggagaactg
     8581 gttaggagta acgaccctaa ttgaacacac taaagatgta ttattgaata agattcatat
     8641 aatatttaat aattttaatt atattaagtg aatacaatat atgtttttag tatcaggaga
     8701 attttgaaaa caaaaattaa aaaatcaagt ataatcagtt tagtaattgc ttttagtatg
     8761 gtgtttactg catttacacc aatagtatct tatgctgacg aagtgactag caatgataca
     8821 atacttaatg gagaagaaca atcaaatggt cagacaccag atgtagagaa accatcagac
     8881 ggtcaggtac cagatgggga gaaaccatca gacagtcaag taccagatgg ggagaaacca
     8941 tcagacggtc aggtaccaga tggggagaaa ccatcagatg gtcagatacc agatggggag
     9001 aaaccatcag atggtcagat accagatgga gagaaaccat cagatggtca gataccagat
     9061 ggagagaaac catcagatgg tcagatacca gatggggaga aaccatcaga tggtcagatg
     9121 ccagatggag agaagacatc agatagtcag acgccagatg tagagaaacc atcagatggt
     9181 cagatgccag atggggagaa accattagat gagcaaactc cagaagaaga aaaaccattg
     9241 gaagaagaaa tagttataga ggaaatgagt ttaaaacaag atattgataa aattcttgat
     9301 atgactttat ctcaaataaa taaaatagta tataactttt gggaagatga agaagatgta
     9361 aaggcagatg aacaatcaga aattaaccaa gtttttactt cagaagactc atttattagc
     9421 ttatggtatg acaaaaaagc aaaagtaaaa aattcttgtt tactaaaaga aatagatggt
     9481 tcagatagac cttatcatga tttaagattt gatgatatta caaagacttt aacttttgac
     9541 tatgtaataa agggtcttgt aggagcaagc tcaaaagacg acaaatatgt aatagaaggt
     9601 gaagatggag agcaaactgc attttgttac aataatcatt taagaccacc aagtagtaat
     9661 ggaaagagtc catatttacc agcagaagat tttaatggtc aagaaaacca aaatgaagaa
     9721 gcagttaaat ctatactata tgcaggttct gaatttgatg gatttggata taagcaacaa
     9781 tttaatttag gtggagaaga aaatgaattg atgacttact cagctactca aagtgcaatt
     9841 tggattattc ttggacaaat ggatgaacaa gaaagactta agcagtatca aggttctata
     9901 aacctttgtg ataaacttat agaaagagca aaaactgaag aggaaagagc tgagtatcag
     9961 aagaagaaag aagtagctat aaatataaaa gcttatttag aagcattgtt aaaagcaggt
    10021 cgtgaagagt taaagccaaa tgatacagga aaacctagtt tgagtaatgg tttaactaag
    10081 attaactttg aaaaaaatga agatggtact tatgaaacag aagctgtagc cttagtaggt
    10141 tatagtggtg tagtaaagtt aagactacca gatggagtaa ctgcttatga tggagatggt
    10201 aatataatag gtacaggtga agtagaaatt tcaacacaac agaaatttaa acttaagagt
    10261 gttgtaaaac cagatgccaa agctaatatt tctgctgtat cttatgatta tatttttcca
    10321 aaagctattc aatattataa ggcagtgctt gatttaggac aaaaagacca ttcatcttca
    10381 ttaccatcta gtaagcaaaa tttattaagt tatacaatag agaaaaaaaa tggtcaagaa
    10441 gttaacttta atataggtct accaacagat gatgttgtaa atccaccagt gccaccaaca
    10501 gatgatacaa atggacataa gccaaaacca tcaccaccaa tagatgatac agtaataaat
    10561 ccaccagtac caccaatgga tgatacaata ataaatccac cagtaccacc aacggaggat
    10621 gcagtgttaa atccaccagt gccaccaacg gaggatgcag tgctaaatcc accagtgcca
    10681 ccaacagagg atacagtgtt aaatccacca gtaccatcag taagtgatac agtggaaaaa
    10741 actccagaac tatcaagaga tgatacaata ataaaatcac caaaaacagg tgatgaaact
    10801 cagctaatgt catatgtatt tattagtgtc attgcaattt gtggattagc gtatcaatgt
    10861 aaaataaaaa gaaattaatc tagattatta ttaagtttaa aataaaaagt ctcaaattga
    10921 tttataagtc attttggggc ttttttatgt aaatataata tgcaaatata gtttgtttaa
    10981 aaatatatat tttaattata ttcttaaatt tatgattgga tacaggcatt cattaattgt
    11041 ttgatgaata gaaaaatgtt ttgaaggtga aaatagtatt gaaaaaataa tatactaagg
    11101 gtataattca tttgatttca tttgtataaa tttaattata aattttaaag tgattatata
    11161 aatttaaatg ctaattactt aataaatttt aataataatt atttaa
//
LOCUS       DAYAOX010000118        11199 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.118, whole genome shotgun sequence.
ACCESSION   DAYAOX010000118 DAYAOX010000000
VERSION     DAYAOX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.118"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..245)
                     /locus_tag="ACGI57_002420"
     CDS             complement(<1..245)
                     /locus_tag="ACGI57_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717495.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITI"
     gene            complement(528..824)
                     /locus_tag="ACGI57_002421"
     CDS             complement(528..824)
                     /locus_tag="ACGI57_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717496.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKNYLNRF"
     gene            complement(843..1256)
                     /locus_tag="ACGI57_002422"
     CDS             complement(843..1256)
                     /locus_tag="ACGI57_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717497.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(1240..1473)
                     /locus_tag="ACGI57_002423"
     CDS             complement(1240..1473)
                     /locus_tag="ACGI57_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9717498.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            1692..2066
                     /locus_tag="ACGI57_002424"
     CDS             1692..2066
                     /locus_tag="ACGI57_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9717499.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
     gene            2678..3370
                     /locus_tag="ACGI57_002425"
     CDS             2678..3370
                     /locus_tag="ACGI57_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9717500.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            3566..5626
                     /locus_tag="ACGI57_002426"
     CDS             3566..5626
                     /locus_tag="ACGI57_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFG9717501.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(5831..6154)
                     /locus_tag="ACGI57_002427"
     CDS             complement(5831..6154)
                     /locus_tag="ACGI57_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFG9717502.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(6147..6848)
                     /locus_tag="ACGI57_002428"
     CDS             complement(6147..6848)
                     /locus_tag="ACGI57_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFG9717503.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(7126..8511)
                     /locus_tag="ACGI57_002429"
     CDS             complement(7126..8511)
                     /locus_tag="ACGI57_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9717504.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(8619..9857)
                     /locus_tag="ACGI57_002430"
     CDS             complement(8619..9857)
                     /locus_tag="ACGI57_002430"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9717505.1"
                     /translation="MELFIEVIKFNIYISPLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNIDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRL
                     DSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(9870..10247)
                     /locus_tag="ACGI57_002431"
     CDS             complement(9870..10247)
                     /locus_tag="ACGI57_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9717506.1"
                     /translation="MIILKKIPKSELLVMNFIWDKDIDKIPAREVANYMEISHGWTKG
                     TTGKVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISS
                     LGCGNEISDDDIKELEQWLKKRK"
     gene            complement(10766..>11199)
                     /locus_tag="ACGI57_002432"
     CDS             complement(10766..>11199)
                     /locus_tag="ACGI57_002432"
                     /inference="COORDINATES: protein motif:HMM:NF023116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFG9717507.1"
                     /translation="ENDMNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTF
                     VQQKNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKR
                     NIFGKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
BASE COUNT         4251 a         1336 c         1481 g         4131 t
ORIGIN      
        1 attattgtaa tatctaatct tcttgtaaat aagcctattg ctatatactc aagacctgtt
       61 acatagcgat aaagtacttc tagtttaaca gtttttttct tatctatatt tccatatatt
      121 gattctaatt tattaaaata ttttttacta caaaaagcag ttttaggttt aattaaatat
      181 attattgtag ttcctattat aactattatt ccaataagta taagattgat atttccaaaa
      241 tccatagcag atacctccta ttaaatttgc ttttcttaat caaagtatat cagtaacaca
      301 agtacaaaga gtatctaaaa tattacaaat attaacagga tagataaaac tatagtaaaa
      361 attgtaataa taaagcgaac gaaaattatc atactaaaac ataagacatg ttataattgt
      421 attagataga agattaacat acaccaaagc actccttata ataaagagtg cttatttttt
      481 tgaaattcat caatatataa actatcaaga accttattca acacacctta aaatcgattt
      541 aaatagttct ttttgataac ataatttata tgatataata aaaaaataga agtgtaccac
      601 caatacactt ctatagctat aactaagcat tctcttgctg ggggagtgct tttttcattt
      661 ctccaaacaa cattatttac ttcatcagta attatcaaat caatctcatt atctttcaaa
      721 atctctgttg cagtacaaat tagattatct aagtcaaaac ttcgagttat ctctagttgt
      781 ttgttattta cttctctttt tagtatataa gttttaaaat acatttatat caccccttgt
      841 atttactctt ttttctctaa aaatatattg ataagtaaat atgcaacaaa taaaaaaatc
      901 tcaatgacac atagtgttat agctataact agaagtgatt gatttttcca tgcactataa
      961 gctatgatga aaagaaatgt aaaagtaaat aatgtataat tcataatttt aactaagata
     1021 tgacttgttt tcattgttaa gtacaagtct ctttcatctg tactatcgtg taattcttca
     1081 acaatgcctt ttcttgaaaa agcattacaa aaattaaaga ttgctattac aattaaaaga
     1141 aatgatgaaa ttaggaatct tttatctttt aaaatgcaat aaataactaa actagcacaa
     1201 cataacacac aaactataat acccactata aaacttcgtt tactctttac tttcatgttt
     1261 tttatcctcc atttctttat tttcttttaa acaacatagt tcttcaatcg ttgtaccaaa
     1321 cacttgtgcc atacgatagg caagcataag agaagggcta tattgctctt tttcaataga
     1381 aataattgtt ctgtttgata catggactaa atcagcgagt tgttgctgtg ttagacccat
     1441 atttgttcga aattctcgta cttttgtttt cattccataa cctctttcta tatgaagcta
     1501 acttcatgtg aagttaactt cataatatcc taaaatgagt aaaatgtcaa tctcattaga
     1561 taagtattat aaaacctaaa agaataatat ggtaaaaatt ggatggaatt atttacctta
     1621 attctcttgt ttgtaaaact ttattgatat agaatttaac atatacataa atcggcaaag
     1681 gggcgcgaat tatgaaaatt agtaaattac cagaagcgga attaaaagtt atgagatata
     1741 tttgtgaagc taacaaggta ttaacttcta gggaattagt tgctgcaatg gaagaaaaat
     1801 atgagtggaa tgaatcaact acatttacag ttttgaaaag attagagcga agagagtttt
     1861 taagtacaga gaaaataggc aaacttacac attataatat actagtaaaa gagaaaaaat
     1921 atctaaggtt tgaaacaaag gaatttttga agaacataca taaaaattca atttcaagtt
     1981 taatgtcagc gttacatggt gaagacgacg aagtggatga agataaatta agtgaactcg
     2041 aagaatgttt taagaatttg aaataagaaa atgacaaatt gatacataat aaataaaatg
     2101 aaggtatgaa attataactt tttctacctt cattttgttt attattctga acatgttaag
     2161 aaaatgaaat aagtttgtat tttagttata taaattttaa tagaatatgt acagtatata
     2221 gtagatagaa tgtataaaaa tagaatttaa ataagtagct attattgcag tgaaatgaat
     2281 gtttctaagt atatttgggt ggaagaatgg tggaagaaaa ttagtgaaat gtaattcttc
     2341 caccattctt ccacctagaa aatagaaact gtttaataag aattaatata cattaattca
     2401 gctgaatgca atcaaaatac ttaaatgaaa gtaatctgaa aacaacataa ttcaaatcaa
     2461 tttattcaaa taaacctcaa tttatcttaa atcttaagaa atatttaaga attaggataa
     2521 tattttctta agatttatat tttattatat agttataagg gaaagttaca aaaatgtaag
     2581 ccaaagtaat ataacaattt agtcattgtt gagaaaggga gtgaaagata ttgtataata
     2641 tagataagta ttattataca atagaggaag tgaaaatatg tacaatatat tagtagtaga
     2701 cgatgataaa gaaattgttg attcgataga aatatatctt aaatcagaag gatttaaaat
     2761 atataaagca tatgatggaa tgaaagcttt ggaaataata acagagaatg acattcatct
     2821 aatactaatg gatataatga tgcctaaatt agatggaatc aaagcaactg taaaaatacg
     2881 agaagaaaga aatattccaa taattttaat ttcagcaaaa agtgaagata cagataaaat
     2941 aataggatta aatattggag ctgatgatta tataacaaag ccatttaatc tattagagct
     3001 tatagctagg gttaaatcaa acttaagacg atatgtaact ttaggtaatt atgaagatac
     3061 atgtaaagaa attctaaaaa gtggagcttt ggagttaaat acactaacaa aagaagttaa
     3121 aattgatggt gaaaacataa aaatgactcc aattgagtat aaaataattg aacttctttt
     3181 agaaaataaa ggacgagttt tctcaataga tgagatatac gaaaaagtat ggaatgagga
     3241 aagttttaat tcagaaaata cagtagcagt acatatcaga cgaataagag aaaagattga
     3301 aataaatcca aaagaaccaa gatttttaaa ggtggtgtgg ggagttggtt ataagattga
     3361 aaaattttag tggaaaaatt ataaaagaaa agagtttaat atttaattgc attttgattt
     3421 tagtcatttt tacatttaca caaatgttag gaattttaat atgttttaat tttttctctg
     3481 agagtttaat ttactctatg gggattatgc ttttcttaaa ctttatttta tataaaatac
     3541 tgacaatgta gatgaggtgg gcaacttgga gaaatataga gtattaaagt tagtttcatt
     3601 aatcataata tctatctcat ttccattaac tatattgttg gtgacaatag gacttgctga
     3661 gcctaaatac aatatacaaa aaacttctac aaagagtggg atttttgata tatctgatga
     3721 tttttatgat agtaatatat ttgacgaagg aacaatggca agtctattac ccaagtttaa
     3781 gctatctgta ggtggtaaat taactagtaa agaaaaaggt aaattccaac aagaaggtaa
     3841 aagtatagat ttaagtagta gtgattctga gtatgataaa atcatgatta atgaacaagc
     3901 ttctgaagaa tatttaagtt atctaaaaaa tataaaagtg tatgttatta atacatacac
     3961 aggagtattt tatacaaata cgtcatatgc tagtttatat gattttaaac aaaaaactaa
     4021 gagctattgt aatgtagaga taataaataa gataggaaag agctcatata ttaaaacaat
     4081 aaatggagaa aaatttgaac tgaataatct gagtctggaa ctaaatgaag tagatgaagg
     4141 ttttgaggca tatgtatctt ttccagaaga gccaactata gaagatggaa tagtatacac
     4201 taattttcaa atatttaaac aagctacaga aaaagtaaga ttattttttg tagctagttt
     4261 aagtttagca attatatgtt tattatctat aattttatat atcaaaataa aaggtgaagt
     4321 tttaaaaaga aataatgtat ttttactaat atacagtaag atacctatgg aatttaatgt
     4381 aatattagta tctatttcaa ttgcaatttt aactatatat gctcttgctg tatcttatca
     4441 tattgatgta attgcattag tttctttatc tttactgata gtatcaaata atatattttt
     4501 tataaatgtg caattgagct atttagaaaa taaaatgtgt atatttaaga caagtattat
     4561 agtaagagtg attttatggt taataaaaac tgtaagggag gtcagtaacg ctataaagaa
     4621 agtatcttta gcaaggaaag ttattgttat agtgataata tctttagctt taatcatggc
     4681 aattagcact cgaagtatag gtatgggatt actgttttct atatgtattt ttttaggatt
     4741 tacaatatat ataactaaaa gactttcata tcttagttac gtaatagaag gtacagaaag
     4801 aataaaaaag ggagaattag attataagat aaaaattact gggaatgata attttacgtc
     4861 tttagctgaa aatataaata atattggaga aggtcttgat agagctatag aagagcaagt
     4921 aaaaagtgaa agaatgaagt ctgaccttat aacaaatgta tctcatgatt tgaaaactcc
     4981 acttacttct ataataaatt atgtagattt aattaaaaaa gaagaatcaa tacaaccaga
     5041 gtatataaat gattatataa atgtacttga gtcaaagtca aaaagactaa aattattgat
     5101 agaagattta tttgaagcga gtagagtaag tagtggcaat atagaattac aaattagcaa
     5161 gattgattta gtgcaactat taagacaaag tataggtgaa cttgaagaaa aattatctaa
     5221 aaataattta tatttaaaat taaatgttcc aaatgataag gtgtatatat gggcagatgg
     5281 aagaagaatg tatagagtat ttgaaaatct tttatctaat atagcaaaat attccttaga
     5341 agaaactaga gtctatattg atgtatatga ctatggtgaa aatgtaaagg taactatgaa
     5401 aaatatatct tcttatgaat taaattttga cccaagtgag attatggaaa gatttaaaag
     5461 agctgatgaa tcaaggaata ctgaaggaag tggattaggg cttgctattg caagagattt
     5521 agtagcttta caaggtggaa aattttatat agaaatagat ggagatttat ttaaagcaaa
     5581 tttagagttt caaaaatatg aagaagaaat aacaaatatg gaatgagata gtatttaagt
     5641 atatatattt ttttactgtc gaaagctttt attataatat attagaatat ttacagtaga
     5701 gaatgaagag taaaaacaga gtaactttaa gtcactctgt tttttattta aaatcaataa
     5761 tataacaact taaattaaaa ttgtacacat agttttaaaa taacttatac aataaaacat
     5821 tatccaaata tcacaagata attccaaata aatatataga tatcactcct gtaataatag
     5881 aaataccaat agatttaaac ttatatgcaa caagaagtac aatcataaaa gatataattt
     5941 tagggttagc tatagataaa tatagactat ctccattaaa aaatacatct ttagcagtta
     6001 aggaagtaag tattgctaca ggtataaatt tcatgatttt tttcaaaaaa tcaggcatct
     6061 ctttttttga aaaaattaac ataggaagca ctcttggtag atatgtaaca atgtacattc
     6121 caattataat aaaaaatata taattattat tcataagatt cctccttttt acagtaagat
     6181 tcttctataa agaaaccaat taaagatgct aaaattgatg caactattat tactaaattg
     6241 cttttgaata atgcatataa aataacagaa ataatgattg atgatataaa tactacaata
     6301 taaatcttat tttttatttg agaaactatt aaagctataa acatagaagt caatatgaag
     6361 ccagaaatag aactgtctat agatatactt tctcctaaaa atgctccaat aaaattagct
     6421 aaaactgatg aaattaagca gaagaaatta agataaatag catttctatc agaccaattt
     6481 ccagcagtaa atttttcata attaactgca aaagtttcat ctgttatagt atgacaaaat
     6541 ggcaatataa attttggact tttatttttt atgtacatgt tcaaattaga actcattaga
     6601 aaatgtctta agttaactat aaatgtagtc aaaacaatag atgttacact tgcaccagta
     6661 gaaatcattg aagctgcaat gaattgagaa cttcctccaa atactaaagt taccattagt
     6721 gctatttgaa aaggtgaaag accagatttt tgtaatagta ttcctcctac tatacctata
     6781 ggtatataac ctaagaatat aggaattgaa gcaataaagc tttcttttaa ttgttgagtt
     6841 ctttccacaa taaatcactc cttttgtatt aaatgtatta aagtattcgt aaaacagaaa
     6901 aatagattaa ttgtataatt gcaattataa ttaaaaataa atttggatta atatataaaa
     6961 tactatacaa caaatagata atcttaaacg gttattaaat tttatctatt tgttattatg
     7021 atgtaataat acatattaat ctctataagt taattactat ctggtgtaat tttattttaa
     7081 caacaaaaaa aaatacacac aattaaaata ttgtgtgcaa tacaattatt ttttctcttc
     7141 taatagtctt tttaatacgc tcaaaccagc tttaagttct tctgtacttt ctgtagcaca
     7201 tacagctatt cttattgcag aaattggttt ttcttttcca acagcaaaac gttcagaagc
     7261 atagacctgt acaccttctt taagtgcttg tatctcaaat ttttctgcca ttattccatc
     7321 aggtagaatc aaccatctaa atatacattc ttcatttccc aaaagattat atccagataa
     7381 atattgatta ataatcttgt ttcttcttct tgcagattta cggcgtgcat taattaattt
     7441 atcggcttca cctgatgtaa tcatgcgata agcaatttct gttaaaaaat aagattgaga
     7501 tagattaatg ttgtataatg cgcttgataa tgcctctcga tactgtttag gtgtagagat
     7561 atatgctagc cgtaaactag gtgcaataat ttttgataaa ctcgtaatat aaattgtttg
     7621 attgggcaga taggaagcca ctggatttaa atgtgtttca ttaagtaaac tatgaatagc
     7681 atcctcaata acaattaaat tgtatttaga tgcaatattt gcaatcattt ttcgtccatt
     7741 ttgtgacatt atatgtgtag ttggattttg ataatctggt attatgtata gacctttaat
     7801 attttcattt ttacaagcat ataatagtcc ttcttcacta atctcattat cttcttgttt
     7861 aattggaatt agttgaatcc caagcatttt agctgctgtc tttattccag gataagttaa
     7921 tgggtctaca cctattctat ctccatgttg aaacaaacca gctaaaatag caacaattgc
     7981 attttgtcca ccacttgcag gaagtaagct atctgatgta gtttcaagcc cagctttata
     8041 aattaatttg gcaatagctt ctttttgcca tttagcacca tctctttgtc catattggaa
     8101 tatggtttta aaatctattt ccttaattat gtgtttaaac aaagtattca tttcttctag
     8161 tgtgaaatct ggattcattg tgcccatttc aataaatgta attttattat tactggacat
     8221 caagaataga tttgaacgtg tatcataaga tacaaaagtt ccacttcctg taactccact
     8281 tattagacct tttttttcac aaattttaaa agctctagag atagtactca cattaacatc
     8341 taagaaatct gccaattcac gttggggtgg taatttagta cctggtaata aaaatccatt
     8401 gttgatatct tgttctagtt gttctgcaag cgccttatat aatatttgcc cttttgaagg
     8461 tctctttggt ttccagttca taggataatt ttcaaaagaa ttaattggca taagttttca
     8521 cctcacatta ttaatttgat tttttcttta tctaaaacaa taaattctaa agatacaaat
     8581 aaaaactgat aggaaataat tctatcagtt tttgtctttt aattgatttc aaatcttcct
     8641 ataattttta taccttcttt atcataaaga gggattgttt ttttaaatat attaaaattt
     8701 ctttttcttg tatataaaac agcttcttct ggtgtctttg gttgattatc acaatcgtac
     8761 aaatctcttt tttttacgta gccgactttt ttacctctac ctattgtttg tataagttct
     8821 ggttcaatgg aatttccatt tgagtctatt atatgtgtac cataggtttc tccattttta
     8881 ttataattaa tcttaccaat agtatattct acatcttcat tttgtttaaa attggagttg
     8941 atagaaaata ttgaccctat tattataaag gttaataaaa acttaagacc taaattaatt
     9001 ttctttttat cttgattaaa catagaatct aacctctttt ttaaaataat atttttttta
     9061 tttagaaact gagatacgaa tgatgaatac ttcaaagatt tttgatactt tattgtatct
     9121 aatattatat atgcatactc tttaatttca ctcgtggtac acttgtcaat cacaatttca
     9181 tcacaagata attcacataa ttcatgaaaa tattttttta ataagtataa aattggattg
     9241 aaccaatata tgatacaaat tatactaatt agaagcttaa ataagttatc ttttctttta
     9301 taatgaatta attcatgttt aaagatgaat cttaactctt tattattgta ctttttttct
     9361 ggaattatta ttttacagcg tgttaaacct ataatggcag gagaataaat acctttaacc
     9421 ttatatatct gaagtttttt atttatactt agttcttttg cttgttcatc taagatacta
     9481 gtaatatcta tttctaaaat ttctttttta aaattttcta tgtaattata aaatttaaaa
     9541 tttgttctta aatagtaaaa agagattgct ataattccta atatccagat gtacataaat
     9601 atatctaaag taatgccctg gtcatatggt atataattat cgatattatg cttatagata
     9661 ttaatagtag tattattgat atttaatata tttaaattta ttttagatat aaacatcatt
     9721 ctgactacta ttaaaagaca tataaaataa ttaaacttaa aagtatattt gcttatcttt
     9781 ttagtaaata aaatgaaaaa aagaattagt ggacttatgt aaatattaaa tttaataact
     9841 tctataaata attccataaa atacgtctcc tattttcttt tttttagcca ttgttcaagt
     9901 tcttttatgt catcatctga tatttcattt ccacaaccta atgatgatat aaagctatga
     9961 atagaatttt tatgtataag ctctaaaaaa tcctttgttt caaattttag atattcctca
    10021 tgtttaactt taatagtgta taaagtattt ctaccatctt tttcaaattc taaaaaacct
    10081 ttatcactta atctagaaag tactttacca gtagtacctt ttgtccatcc atgagaaatt
    10141 tccatataat tagctacttc tctggctggt attttatcta tgtctttatc ccatatgaaa
    10201 ttcataacta acaactcgga ttttggtatt tttttcaata taatcactca cctttaactt
    10261 gattatatta gacaaatgtc taatagtcaa tataatatat catcatgtat aaataatctt
    10321 tatattattt tatgcttgag aactaagtag tatagattta tagataaaaa agatattgag
    10381 agttttatgg aagaggggaa tatattttct attagagata agaatatgac atatattttt
    10441 tattgatttt tacaaaaata ctatatagtg aataaattaa tatcttttct aaattagaag
    10501 aggagaatct tattagataa agattatcct ctttattgaa agtttaaggg atattagaga
    10561 ttattaattt ttctctcttt ttttaactaa aagcaataat acataaaatt attattgcta
    10621 atgatactct taatgtggta tatagtcata gtgtcacatg tttatcaata gttttttcat
    10681 taattattgt aacgaatagt aggactcata aaatacttag aaatatgttt ttagatagat
    10741 tttaaatata taatctaact tatatctata aattaacact attattctcc aattccattt
    10801 ctagtttttt tataaattta ttaaattctt ctggttgcct ttttccaaac aatactctta
    10861 caagagctga attgattttt ccaaatatat tcctcttgct ctcactttca ataaaaataa
    10921 gttttgtatt ttctaagtca atatgtacta attcatatct agtaatagta ctttctcttt
    10981 tattgcttgt ttttatctca tatactctat ttttttcgaa attagttatt tcaaccttaa
    11041 atcttttatt atttttttgc tgtacaaatg ttcctactgc ttttttctca ttaaaattag
    11101 gaaaatttag tcttgcagat tttgtaaata tattaaatat gtcttttgca gaatatttta
    11161 ataaaacttc agttttaaat gtattcatat cgttttccc
//
LOCUS       DAYAOX010000119        11186 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.119, whole genome shotgun sequence.
ACCESSION   DAYAOX010000119 DAYAOX010000000
VERSION     DAYAOX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.119"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            73..1554
                     /locus_tag="ACGI57_002433"
     CDS             73..1554
                     /locus_tag="ACGI57_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFG9717508.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1587..5021
                     /locus_tag="ACGI57_002434"
     CDS             1587..5021
                     /locus_tag="ACGI57_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HFG9717509.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDGIEKGIWILNDI
                     TDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNVP
                     YSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDED
                     GNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESWQ
                     KPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRRM
                     NQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYNK
                     GTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPIF
                     RKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGIS
                     FVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADKC
                     EKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDRP
                     CNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5039..5785
                     /locus_tag="ACGI57_002435"
     CDS             5039..5785
                     /locus_tag="ACGI57_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFG9717510.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5734..6042)
                     /locus_tag="ACGI57_002436"
     CDS             complement(5734..6042)
                     /locus_tag="ACGI57_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFG9717511.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            6846..10976
                     /locus_tag="ACGI57_002437"
     CDS             6846..10976
                     /locus_tag="ACGI57_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HFG9717512.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTSPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINPDNGTF
                     GFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSNVIGA
                     WWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVTGYPP
                     PQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFSEGES
                     AIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTNVFFR
                     DLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPNPNPT
                     NNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEELIYTI
                     KVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETRVIII
                     RGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNSAVPG
                     TLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGTLNSG
                     ISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISIIKVAD
                     EDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWTGSLN
                     IGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSADIEV
                     IKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVTFQPW
                     NGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDETDIS
                     PLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYSIDNG
                     VTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNNNIST
                     SEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPASILSP
                     EYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPN
                     PDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTLIDNV
                     SDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNIAEIT
                     STTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDFWC"
BASE COUNT         4521 a         1336 c         1767 g         3562 t
ORIGIN      
        1 aaatacttat taatagtaaa ataaagtgaa tcaattatat tataattgct aaagagagtg
       61 aggtgtagct atttgaaaaa aaatagatta atagcaaaga atatatcaat agcatttata
      121 gtactattct tttttagtgt atttacattt ttttatgtag gtaatattaa tagagtgtta
      181 gaatatgaaa caaatgacat aataacagtt acaattgctg gatggattat actttcattt
      241 ttatttttag gtattataat ttacatatta tattcaaaag caaatagtca aaaaactata
      301 gaaaaagtag catatacgga ttttgtaaca ggttattcca actggagaaa gtttgaatta
      361 gatgttacaa atttattaaa gaaaacatca caaaataata aatatgcaat ggtcatattc
      421 gatattgata aatttaaagc aattaatgat atatatggcc ataaaaaggg aaatttaata
      481 ttaaaagata ttgcagatac tttaaatgaa ttgactgata tcaatgaaac ttttgctcgt
      541 gtaagtgctg ataatttcaa tatattatta acatataaca aaaaagagga tataataaat
      601 attatcaaaa agattatggc aaataatgaa cttgtaaact tatcttttgg aatttatgaa
      661 atcaaagata aggatttatc tgtaagtgta tatagtgata gagcttcact tgcaaaatca
      721 tcaataaaaa ataatagtga tgtaaatttt gcatttttta atgacaaatt aagagagaaa
      781 ttattatttg aagataagat agaaaaagaa atggaatatg ctcttgaaag tgggcaattc
      841 gtaatgtatt tacaaccaaa atacaatatt aaattagata aattttgtgg aagtgaagcc
      901 ctagtcagat ggcaatatac agaaaaagag gtcatatatc caggtgattt tattcctata
      961 tttgaaaaaa atggatttat tagaaagatt gatatgtaca tattagaaca agcttgtaaa
     1021 gaaatccggt ctttatttga taaaggaatt tcacctttac ctatatctgt aaacttctca
     1081 agagttgatt tttttaaaaa agattttatt gaaaacatag taaatatatg tgatagatat
     1141 aaaataccat atagtttaat agaaattgaa attactgaat ctagtatgtt tggagatact
     1201 gatactctat ttaatgttag tagaaaccta caggatattg gctttatagt tgcaatggat
     1261 gattttggct ctggatattc ttctgtaaat atgcttaaaa atataccatt aaatgtaata
     1321 aaattagata gaggattttt tgttgatgat aaagatgttg ataagagtca aattgttata
     1381 aaaagcatag tttctctcat taagcaatta ggaataaggg ttgttgctga aggaattgaa
     1441 acaagaagtc aaatagaaat gttaaaaaaa gcaaattgtg atattgttca aggatattat
     1501 ttttcaaaac cattaccaat taaagaattt gaaaaattag tctataaaat atagtcagcg
     1561 agtcttaaat gggaggtggt gattaagtga taagagcaaa atcgttaaaa aatgtactga
     1621 gtattgtttt aatattttta tttacaataa tttctttttc tgattttgca tttaaaacta
     1681 atgtccagga agaagaaaat agtaaaatta atttaagaga aatatcccta caaagttcca
     1741 atgctataaa aataaaatta gatagttctc tatcctttat taaaagtgtg gcaaatatgg
     1801 tttcttattt taatactata gataactcaa aaatagttcc attattagct aatatatcaa
     1861 aagaatctga ctttcaaaga atctctattg tatctcctga tggaataagt cataccagtg
     1921 atggcaaaat tatcaatgtg tcaaagtatc agtattttaa gaaagctatt aaaggagaaa
     1981 gtaatatatc tgaagttgtg aaatctccaa ttgatggaaa agatactttt gttatagcta
     2041 ctcctgtatt taataagaat aacaaaataa ttggtgtttt atacggttct tactacaatg
     2101 ataaattagc tgaatttata gatataacaa gttttaatag tgaagggtac atagatatat
     2161 ttgaatctag tggtcaattt gttttaaaat cttcacatac aaacaaactg attaagtcaa
     2221 gtagtaacat atatgatata tttaaaaaag cttcttttga tgatggttat agctataaaa
     2281 aaatatatga agatacaaaa aataacaaga gtggctttac ctcatatgaa tacaaaggag
     2341 aaaatcgtta tgctacttat acacccattg gtgtaaatga ctggtatgta atttctgtga
     2401 taccacaata tactgtaaga agaaacattg ataaaataaa tcaattagca ttttctctta
     2461 ttttcaaggt attcttattg tttattatgc ttataatata tattatttat aaaaacaata
     2521 aatctcagaa agatatacta aaagctaaca aagaaatctc atctattaca aatagcattc
     2581 ctggaggagt acaaaagtgt accctgggtg aaacatgcaa tattaatttt ttaagtgatg
     2641 gatttataga gttgtttggt tatacaagag aagaaataaa aacattgttt aataatgatt
     2701 tttatagttt aatacataaa gatgatgttg aaagaataaa aaaagaaatt tttaaacaac
     2761 tttacaaaaa taaaactgtt gaattagaat ataggattat aacaaaaaat ggtaatacag
     2821 tatgggtttt agataaaggg gagcttataa cagaacctga tggaaaagaa tatttttgtt
     2881 gtgtaactgt agatattact agaacaaaac aattagaaca agagatggaa caaaaaaatc
     2941 tagaacttga aatgatatca gaaaatgttt taggtggtat aattataaca gaatttaatg
     3001 atgattttac tataaaatac gctaactctg gttatctttc aatgattgga tatacacgtg
     3061 aacaattaga aaatgaactt aaatacaaag cttgtaatct tgttttttat gaggataaaa
     3121 agaaggctct tgaaagtata caacatcaat taaacacaaa aggaatagct gtatatcaac
     3181 atagaataag aagaagaaat ggtaatatta tttgggtttc tataaaagga aggcttattg
     3241 ttgatgatgg tattgaaaaa ggtatttgga ttctaaatga tataacagat ttaaaaaatg
     3301 cagaacaaaa acttaaaata aatgaagaaa gatttagaat tgcattaaag caaacatcaa
     3361 acactatatt tgagtatgat ataaaaacaa agtcactttt aaatactagt aaagagcttg
     3421 ataaatataa tatttataaa attactaaaa acgttccata ttctctagta gaatcaggag
     3481 ttattcataa agattttagt gatgatttta taaacatgta tgaaaatgta ataaatggag
     3541 caaaaaaagc aagttgtgta gttaagatga aacttccaga tgaaaattat atatggaata
     3601 aagttacctt gactactatt tttgatgaag atggtaatcc tgtaagagca attggtatac
     3661 aggaagatat aagcaaacaa aaggaagcag agataagata caatcaagaa ggtaagtatc
     3721 attcagctat attgtcggaa gcaatgataa cttatgaaat aaatgtatca aaggatactt
     3781 ttatcaaagg aaatgaaagt tggcaaaaac catttggaat taagcctgac aataactatt
     3841 ctcaaatggt aaaaactata gcaaatagta ttgtacataa agacaataaa gaaacatttt
     3901 taaatacatt tttatgtgaa aacttattgg atgcatataa aaataatgtg actgaaataa
     3961 aattagaata tcgaagaatg aatcaagaaa ataaaatgat ttgggtcatt tgtacaatgc
     4021 acttaataaa agattctatt acgaacgata taaaagcact tgcttatgtt aaagatattg
     4081 acaagcaaaa aaatgaagaa attagattaa aattaaaagc tgaaagagat actcttacag
     4141 gattatataa taaaggtact acagaaaaat tgattaatga tttcttgaac tctgaggaag
     4201 caagcttagg aatgcatgca tttttaattg ttgatataga taattttaaa tctgtgaatg
     4261 ataatttagg acatgtattt ggagataaag tattatttga agtttctaaa aacctaaaac
     4321 ctatatttag aaaagatgat atagtaggta gaattggtgg agatgaattt atagtattct
     4381 taaaaaatat tcagtctttt gagaatatca aaaagaaagc agaaaaaata tgcgatatat
     4441 ttagaaattc ttatacagaa aataaaaaga catataaaat atctggaaca gttggaattt
     4501 catttgtacc agaacatgga actgtatttg aagaacttta taaaaaagca gacatagcac
     4561 tttattatgc aaagagcaaa ggaaaagata agtatgcact ttataatgat tgtatctacc
     4621 aaaatgaact tgaaaataat ataagtgata ataatataaa attagcagat aaatgtgaga
     4681 aatccaaacc agtacttaaa gaaattcttg attctattga taattatatc tatatagtga
     4741 atccaaatac ttatgaactt ctttttgtca ataataaggt gtctcgtttg gatttagaga
     4801 taaaattagg agaaacctgt tataaatcac tattaaaatc tgatagacca tgtaataatt
     4861 gccctcttaa cggattagaa gaaaataagc attcaaaatt caaaatagag acaaatacat
     4921 ttggtaaaaa tatcagtata gcaagttgga ttaattgcat agatggagaa tacaactgtc
     4981 ttatagagtg tatagattct tctttataca aaaataaata aaaaaggact atgaagatat
     5041 gttaaatagt ataactttga tacttggtat aatatttaca atttactatt tttatctcaa
     5101 aatagttttt ggaggaattt catttagtga gattttctta gttgtaggaa ttgtactaat
     5161 tatctatcaa atatttaaga aaaaaattaa agaaaagaag gttttttata gggtgttaaa
     5221 aataataatc agcatttttt taatagtatt tgtagttact gagagtctaa ttatttttta
     5281 tcccaaaaat agtttagaat caaaaagtga ttatctctta attttaggag cttctgtaaa
     5341 aaagactaca ccaagtacaa ccctgaaggg taggttagac actgcattga aatacctaaa
     5401 aattaatgat aattgttatg tggtagtatc tggtggtaaa ggaagtggag aaaaaataac
     5461 tgaggcaaaa gctatgaaag attatcttat aaaaaatggt atagataaaa ataggataat
     5521 agaagaggat aaatccacga atacttatga gaattttaaa tattcaaaat tgaagataga
     5581 agaacatagt caaggaaaac taagcgattt aaaagtaaaa atagtaacaa cagattttca
     5641 tgttctcaga agtaaaatat tagcatatag aaatggatat aaaaatacgt ctttttatgc
     5701 tagtaaatca aaactttcat ttgtaccaac ttactataca agagaatttt ttgcattatg
     5761 gaaaacaatc atttttgata gataataaat aaaaagacta gatataaact catatctagt
     5821 cttttctatt cccttaattc taatccattt tccaacttta cttgttgtag aaatttcaac
     5881 attgggattt acaagtaatt gcttataaga agttttatat tttacgattc caaaatataa
     5941 ttttccttca taatccatta taaatccgaa tggtcttact cttggtttat tatcttcaac
     6001 tgtagcaaaa tagaatgttg agttttcttt aaaaaaattc attatttttt catgattaaa
     6061 tcctctttgt atcttatact aatataatga atataaataa aatattcata atactaatat
     6121 aaatcgatat aaaaacaatt acaatattat attgattatt caaaattata tataaaattc
     6181 agaaaaaaat tttagtttac ataataaaaa taaagtcaac taaaattatt gattttagat
     6241 taaagtaccg gattaagcat ttaagtagat tttaagcttg atttgattag aaaggtacaa
     6301 actatcaccg aaaaaaatta aacccattta aaaagcaata taaaagcttg ttatttttga
     6361 gaaaaataga ccctctaaac tcaattagat taaaaataaa atacagaaaa ttatatctct
     6421 aaatatctta aataattaag tttaaatttt acgatatttt gtttgatgat ttataatata
     6481 aaagcatact cttaatcttc aaaatttaaa aagagtaaaa aataacaaaa aaatcactga
     6541 catttcactg acaaattgta attttgttat tataaataca ttgaaaatac ttgattattg
     6601 aaaaatggca taaaataata cattttatgc catttgctat tatttgatag tttttaactt
     6661 atgacataag atatttttta acatatacaa taaaatataa aaaaattgat attgcaaata
     6721 aaaaaaaata catattagat gttatagaaa aaaagcgcac ttataggctt aaaatgtcac
     6781 ataaaagagt tttaaagaat aatatattat gaacacaaat tttgtaatca caaaggagtg
     6841 ataatatggc aacagtatca ggacaaatag tttttgatag aaatagaagt gctaccattg
     6901 attctggaga ctcaggaata gccaatatac cagttgtatt acaagatata gcaactggag
     6961 ttagacttgt ggttctcaca gatgcatcag gaaattatgt ttttactaat gttccaaatg
     7021 gtaactatag aattgtagaa gcttttggaa ctacaggagg agtaccaaca ccaggaaatt
     7081 ttgcaacagc gatagtaggt ccagttccaa ttgcagaaat gccacctatt agttctgtga
     7141 caagtcctcc tattggttca acaaacttag atggtacaac acctaacaca ctattaataa
     7201 cagtagcagg ttcagatata acaaatcaaa atatattaaa tgggcctgtg atttatacac
     7261 ctatagagaa tatattggat tcttgcgtat ctgtatctaa tataaaccca gataacggaa
     7321 cattcggttt ttttccacca ggtacaccaa gtaatacagg acctgaaata gaaccttacc
     7381 caaatgtgac gccagatttt acgtatgttg taccagaccc aactaagttt acacctttag
     7441 atggagaata tacagtacaa aatattatga caaatgcaat gagtaatgtt ataggagctt
     7501 ggtggcgtat tgctgaccat actacaggaa atgaaacagg aagaatgatg gtggtaaatg
     7561 gttttaatcc tggagctgta ttttttaaat caacagtaca agttacacct aacactaatt
     7621 atttatttag cacttggatt ttaaaccttt ttaaggtaac aggatatcca ccaccacagc
     7681 taggtgtaag aatatttgac caaaataata atatactata tcaagcaaca ttaggagctc
     7741 aaattccagt taatacaaac gcaccagaat ggaaagaagt aggtactgtg attaattctc
     7801 aaaataataa tcaacttaca gtagaatttt ttagtgaagg agaaagcgcc attggaaatg
     7861 actatgctat agatgatgtt tctttaagag aaatagctgt tccaactttt actccaataa
     7921 aaacaagtaa cacagaaact gctaatgtag gtgatataat cacatataca gttacattag
     7981 ataatacttg tgatagccct ttaactaatg tattttttag ggatttaatt ccaaatggtt
     8041 tagagtttat acctggaact gtaacggttg atggagtatc tgtaccagga gtagacccca
     8101 atacaggttt cccacttcca gatattggag gaggaacagg ggttgaagta acttttgatg
     8161 ttgtggttga aagtattcct aacccaaatc ctactaataa tatagctact atagattact
     8221 cctatacacc tgtagaaggc ggtataccaa atgatttttc agtagattct aatccagttc
     8281 ctgttgaagt aatctcagca gatattgaag ttacaaaatt atctgaacct actatagtga
     8341 atccaggaga ggaattaata tacactataa aagtagtaaa taatggacca ttcccatctg
     8401 aaaatgtggt tttaacagat gatgtaccag cttctattgt aaatccagag tattcattag
     8461 atggaggagt aacttttcaa ccatggactg gaagtttaaa tatagggaca ttagaagtag
     8521 gagagacaag agttattata attaggggta ttgtaaatcc atcaacagta ggaattataa
     8581 caaatacagc tgtagtatct tcaactacag cagacccaaa ccttaataat aatacatcta
     8641 caatagagac agaagtgaat ttattagcag atattttagt aatgaaaaca gcagaaccta
     8701 attcagctgt tccaggtact ttattgagat atactattca agtagaaaac ttaggaccag
     8761 caaatgctga aaatgtaata ttaaatgatg atattccagc ttctattata aatccagaat
     8821 attcattgga tggaggagca agttttcaac catggaatgg aagtcttaat ataggaactt
     8881 taaactctgg aatttcatta acagttttaa ttcaaggaac agttagttta aattcttcag
     8941 aatatatagt aaatacagca acagtttctt caactacacc tgacccagat ttaagcaaca
     9001 atatatctac cattattaca ccagttaacc cacaagcagg tatttctatt ataaaagtag
     9061 ctgacgagga tgtagctgtt ccaggagaag aatttgtata tacaattgaa atttttaatg
     9121 aaggaccatc taatgctacg aatgtagttt taacagatga tataccagat gttattttaa
     9181 atccagaata ttcattggat ggaggagcaa ctttccaacc atggactgga agtcttaata
     9241 taggtacagt tgcaccaggg caattaataa gaattatcat aaagggatta gtaagttcaa
     9301 ctgctacagg agatattact aatacagcag aagtaagtgc agatgtacca gaaccagtta
     9361 cggattcatc aacagtgaca actcctatag ttccatcagc agatatagaa gtaataaaaa
     9421 ctagcaatat ggatacagca gttccaggag aaactttcag ttatactata acagttacaa
     9481 atttaggacc atctgctgca caaagtatcg ttttgacaga tgatatacca gatgttattt
     9541 taaatccaga gtattcattg gatggaggag taactttcca accatggaat ggaaatctta
     9601 gtataggcac tttggatgct ggagaaataa gaagtattat aataagagga acagtgagcc
     9661 agacagcagt aggtacaatt ataaatacag caacaataag ttcaactaca cctgacccaa
     9721 atccagataa taatacttca acagatgaaa cagatataag tccattagca gatatttctg
     9781 tgataaaagg caacgaacca gttgctatac cagggggaag atttatttat ggaattgaaa
     9841 ttgcaaatgc aggaccatct tttgcagaga acgtaacttt aacagataat ataccagctt
     9901 ctattttaaa tccagagtac tcaatagata atggagtgac tttccaacca tggaatggaa
     9961 gccttaatat aggtacttta gatgctggag agataagaaa tatcataata aggggaacag
    10021 taagtcaaac agcaataggc acaattataa atacagcaac agtaagttca actacacctg
    10081 acccaaatct aaataacaat atttcaacat cagaagcaga ggtaagttca tcagcagata
    10141 tttctgtagt aaaacgaagc aatcagactg ttgttgtacc aggagatgta ctagactata
    10201 caattgaggt gagaaatgca ggaccgtcta ctgctcagaa cgtaacttta acagataata
    10261 taccagcttc tattttaagt ccagaatatt caatagataa tggagtgact tttcaaccat
    10321 ggaatggaag tcttagtata ggcactttag atgctggaga gataagaaat attataataa
    10381 ggggaatagt aagtcaaaca gcaataggta caattataaa tacagcaaca gtaagttcaa
    10441 ctacacctga cccaaatcca gataataata cttcaacatc agaaatagat ataagttcat
    10501 cagcagatat ttctgtaata aaattagcaa ataaaacaga agcatgtgta ggagagcaaa
    10561 tagattttgt aatagtagta tcaaatgcag gaccagaaaa tgctcaaaat gttactttaa
    10621 tagacaatgt ttcagataag cttaagaaag cagtattttc acttgataga ggtgtaagtt
    10681 tccaaccatg gactggaagt cttaatatag gtacactacc agctggaact ttaagagtga
    10741 ttatattaag aggaattata aaatcaacat gtcttgatag attaacaaat attgcagaaa
    10801 ttacttcaac aacaccagat tcaaacctta acaataatat atcaagagtg caagtagaaa
    10861 taaaacaatg ttgttgtaat gattgctgtt gtaacaactg tagtaattgt tgttgcaatg
    10921 attgtggttg taaagattgt tgctgtaatt gttgtaaaaa tgacttttgg tgttgatttt
    10981 ataaaataat tttgtatacc ttaaattaaa ctgaaataaa aaataaattc acatatgagt
    11041 aaaatatttt ataaatatta ataaattttc tatctttgat atggaccaca ttcgtaatac
    11101 tagttattaa atgaagtggt cctttttaat tcattaaaat tatttattct aattaaattt
    11161 taaatgtatt aaataatttt aatttt
//
LOCUS       DAYAOX010000120        11136 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.120, whole genome shotgun sequence.
ACCESSION   DAYAOX010000120 DAYAOX010000000
VERSION     DAYAOX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.120"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            218..2263
                     /locus_tag="ACGI57_002438"
     CDS             218..2263
                     /locus_tag="ACGI57_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9717513.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            2524..3723
                     /locus_tag="ACGI57_002439"
     CDS             2524..3723
                     /locus_tag="ACGI57_002439"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HFG9717514.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            3775..4470
                     /locus_tag="ACGI57_002440"
     CDS             3775..4470
                     /locus_tag="ACGI57_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HFG9717515.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            4483..5160
                     /locus_tag="ACGI57_002441"
     CDS             4483..5160
                     /locus_tag="ACGI57_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717516.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            5271..6872
                     /locus_tag="ACGI57_002442"
     CDS             5271..6872
                     /locus_tag="ACGI57_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9717517.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCYGKIITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            7321..8610
                     /locus_tag="ACGI57_002443"
     CDS             7321..8610
                     /locus_tag="ACGI57_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFG9717518.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            8639..9319
                     /locus_tag="ACGI57_002444"
     CDS             8639..9319
                     /locus_tag="ACGI57_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9717519.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            9420..9959
                     /locus_tag="ACGI57_002445"
     CDS             9420..9959
                     /locus_tag="ACGI57_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HFG9717520.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            10135..>11136
                     /locus_tag="ACGI57_002446"
     CDS             10135..>11136
                     /locus_tag="ACGI57_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HFG9717521.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     "
BASE COUNT         4445 a         1071 c         2042 g         3578 t
ORIGIN      
        1 cgaatttata ttaaattttt atttatattc caataatgtc aaactaaccg ttttagaaat
       61 taaattatat gtgaaaaaag atagcttaca ttattttatt atggaaaata atagtaaatg
      121 gtacaaaatt tgatttttaa gatttaaaac ttttttaaaa agtgctacaa tagaccaaat
      181 tattactttc tatactcgtt aaacaggagg tgggactatg aaaaaacata atattttatt
      241 tgtatcaact gatgacaaaa taaatataga tatatcaaaa caattggaaa atatatttgg
      301 agaattttgt aatatagata acttggtata tgtaaatagg ataaatattg aactgtcaag
      361 ctatgagctt gtagtatgtt cagacaatga tataaaggaa tatatacata ataatattga
      421 taaaaatata cctattgtaa tagtacatcg tactataaat attgaaaata taaatcagat
      481 aataagtata gaaaatgatt ctgatgttat ggttatagat gcttataaag agtcagcaga
      541 tgaaactgcc aagataatta gaaaactagg attaattcat attaatctaa taccatatta
      601 tccaggttgt gataagtcta aatgtgagat tggaattatt acgggaagca gaaacagcat
      661 accacaaaac ataaagcaaa taattgacat aggtgataag gtaattgata taaatacagt
      721 tatagaaatc tttacaaaac taaatatatc tatagataaa ctacatatta ttaaggaaaa
      781 atacaatgaa gatacagtaa gtggatatag gtattataca actatgaata aaaccatgaa
      841 gagttttttg gagattatag atgaaggtat tgcttctgtt gataaattag gaaaatttat
      901 atattgcaac aaagtatttt ctaatctagt tggaatagac caaaatgaaa ttatatctaa
      961 taactttatg gacttattta gtgataaggt tgtcaaaaaa atattttttc aagaagatga
     1021 agttaatgat gaagtagtaa atttaaataa caaaaaactg ataattaata aagtaaatgt
     1081 ttatgaaaat aatgaacgaa taaagagtat tataagcatt aaaaatataa gtgcaattca
     1141 agtgcttgag gacaagattc aaaataaact tcagtcaaaa ggttttgtat caaagtatac
     1201 atttgagagt gttgtaggtg agagtaaaat aataaaagaa aaaattaata ttgcaagaaa
     1261 aatagcaact acagactttt ctgtgttgat acttggagaa aatggaacag gaaaagaaat
     1321 atttgcccag gcaatacata atgaatctct aagaaaaaac aagccatttg ttgcagttaa
     1381 tctatcttct ttgtcagata cacttattga aagtgaactt tttggctatg atgaaggaag
     1441 ttttacagga gcaataaaag gtggaaaaat gggtatattt gaaagagcac atacaggaac
     1501 aattttttta gatgagatag gtgatatatc tttagatgta cagcagagat tgctaagagt
     1561 attacaggaa aaagaagtta tgagaatggg cggaagtaaa attataccaa tagatgttag
     1621 aattatagct gcaacaaata aagatttaaa gaaaaaaatt aaagaaggtt cttttagaga
     1681 agatttgtat tataggatta atgtattaca tatagagata cctagactta gagaacgtaa
     1741 ggaagatata cctcttataa gtaagtattt tttagatgaa ataaatagca acaaatgttt
     1801 tacagaggaa tcaatgcaag ctttaacatt gtatgaatgg ccaggaaatg taagagagct
     1861 taaaaattta atttattata ttgatactat agttgaggaa gatagagtag attacgagca
     1921 tttgccagaa caatttagat ttgaaaaaaa caatacttta gttaatgaaa atttcgattc
     1981 aattatctta gatttcaaac aatctaattt ctttgaggaa agtatatgta tattgaccag
     2041 tatagaaact tggaataata aaaatatatt attaggaaga aataaactgc aagagatttt
     2101 aaaagaaaaa gatatagttt taagtgtaga ccaaattaga aaaagaatag ataagttaaa
     2161 gagccatggt ttacttttat ctggagtaaa aaaacaaggt agttttataa ctgatgaagg
     2221 aaagaacttt atatcatatg taaaaattaa gggagtgata taggggacta aaaaggcaaa
     2281 acaccctaaa aaaatcccta taatggatag aaataaataa aattatcttt aagattaaga
     2341 taattcaaaa atattgaata aataatctaa gaagattatt tttaataaaa cagtagtaaa
     2401 tatcaacctt actactgttt ttattttttg caagatttat tatacaatta aaatttatat
     2461 tttggcacat caattgctac ataggaaaaa tataaaaaat tgtagaactt ggaggatgtt
     2521 gttatgaagt cagcttacaa aggatatgaa gcctatagtt atttagaaaa aggaaaagat
     2581 atttcatcta ttgagatgtg taaaggagat aaaagagtta aagaatacct tattgaatta
     2641 gatgatattc aagaagctaa ggttaaaagt atagtggatg aaaaaatatt tatatcatta
     2701 catgaacatc cagtattatt tccagaacac ctagaaagag atatatttga atataatagg
     2761 gaaggtaaac aaagatgtgc ttatgaagca ttatcaagag gatattatga tgctatattt
     2821 gataatttaa tggatggtgt atgtacgata acatcaaatt cagggtggaa atgggatgat
     2881 atattaatag atttaggcat gagactatgt gatttagctc atcaagattt tgttataaag
     2941 tgtgaaaaag tagaagatat atacagagct aaaagtgaag gtaaagtagc attgatacca
     3001 acattagaag gtgcagcacc aatagaaaat gaacttgata gaatagatat actgtatgga
     3061 tttggtataa ggttaatggg tataacatac agcgaatcca atgcattagg ttcaggacta
     3121 aaagaagaga aagatggagg tcttacaaat ttcggaaaaa aagctgttga aagaatgaat
     3181 aaaattggaa tggcaataga ttgctctcac gttggagttc aaactacact tgatgtaata
     3241 gaatatagca aggacccaat aatattaagc catgttgggg caagaagttt atggaacaca
     3301 aagagactaa ctccagatga agtattaata gcatgtgcta aaaaaggagg ggttataggt
     3361 gtagaagcag cacctcatac tacaataaca gagaagaata aagaacattc aatagagtca
     3421 tttatggaac attttgagta tattaaaaat ttagtaggaa tagaccatgt agcttttgga
     3481 ccagatacag tatatggcga ccatgtaaaa ttacactcag tactagctgc taaatttgat
     3541 ataaaaaaaa taacaggaac aaaagaacat cccaaagtag aatatgtaaa aggtcttgag
     3601 aacccaacag aaacttcaaa aaacattgtt agatatttag taaagcatgg atatagtgat
     3661 gaggaaatag agaaggtact aggtggaaat atacttagag tactaaaaca ggtttggaaa
     3721 tagatatatt agaaatatta ataaaatttg aggatttata gggaggtttt ataaatggat
     3781 tatttaaaat tagctaatca tccacttttg tggatagcga gtacagtagc agtatcaata
     3841 gtagtggtac aatcccttat atttgctata aaatcttgca aagttggtaa aacaatggga
     3901 ataacagata aacaaataaa gtcagctgtt aagagtagtg ctatttcagc tattgggcct
     3961 tcaatgacta tatttgctgg aatggtatcg cttatagtaa ctatgggtgg gcctatagca
     4021 tggatgagat tatcatttat aggttctgta atttttgaat caatgtcagc cggatttgga
     4081 acagatgcat taggtataac tttaggttct cctgaaatga caaaacttgc atttacaaat
     4141 gcagtttgga ctatgatatt aggttcttta ggatggataa tattcacatt actatttgga
     4201 cataaactag ataagataac caatgttata tctagtggca agaaatcatt tataccaata
     4261 atatctctag gagcaatgtt gggttcattt gcatatctaa atgctgatag ggtgttgcgt
     4321 tttgataatg gaacaattgc atgtataagt ggaatggcta taatggtaat attgtgtatg
     4381 cttgaaaaga agaaaaatat aaaatggtta agagagtggg ggcttacaat atcaatgttt
     4441 agcggaatga taatagcttc agtaatataa ggggggagtt ttatgagtaa agtaaaatct
     4501 atatacaatg aagaatattt accttttatg ataaggtatg gaagactaac actatctttg
     4561 ggtattatag cagcattagt tcctggaata attttgagct ttggatttgg tattatgcct
     4621 ccaatatcag cattattagc aagtacaatg gcaattgtca gtatgagtgc tccaaattac
     4681 ataatagaac cagtttctta ttcaccaatc ttaggtatcc caggaactta tatgagtttt
     4741 ctttctggta atatatctaa tatgaggcta ccatgttcaa tagcagctca aaaggcagca
     4801 gaagttgaat caggtacaga agaaggctcg ataatatcta caataggaat agctgtatct
     4861 attttagtga atataagtat actaactata ggggtaattt taggaggaag cgtactatct
     4921 aaaattccag ctgaagtagt agaaaaatta aacttaattt taccagctct atttgggtca
     4981 gtttttggac aagtattttt acaagataaa aaattaggtc tagtagcaat tgtaatatct
     5041 gtattgacta ttatattatc aaagcaaggt ataataccac agtctctagt agttcttata
     5101 tgtgtatttg gtactatatt aattgcaaga gctatgtata aagataagct tagtgactaa
     5161 ttacaatgaa ataatagtga attttcaaat atgaaattta ataatttgat tttcaaatat
     5221 aaaaattaca ttaagattat ttaaaaattg gtttttaagg agggaatatt ttgatatatg
     5281 atttaatctt aaaaaatgga tttataatag atggtacagg taatcctggt ttttatggag
     5341 atatagcaat taaagacaac ttaatagcaa aaatagattc taagataaat agcaatacaa
     5401 ataaagagat tgattgttat ggaaaaatta taacacctgg atttattgac cctcatgttc
     5461 atgaagaaat agtagcaata ctagatggta aatttgaaaa gtttctaaaa caaggagtta
     5521 caacaactat aaatggtaat tgtggacatt ctataacacc atattcttct gaaaatgtat
     5581 atgaatatat gtataaaaat ggtctattac ttgaagaaga aaaaaagtat cttatagata
     5641 aaaataaatg ttggaataat tttacagaat attgtgacct tatatctaaa agtggtattt
     5701 ctgtcaatat gggattcttg ttggggcatg ggactataag atggagtgtt atgggaggtt
     5761 ctaaggatag acctcctaca gaaaaggaaa aaaatgaaat tacagatata ataaatgatg
     5821 gaatgaaaag tggtgctttt ggaatatcta ctggattggc atatatacca agtaagtatg
     5881 ctgatattga tgaacttgtt gatattgcaa ggcagattaa agagcatgat ggtatatata
     5941 catctcatat tagagattac ataggaagat ataatgcagt aaaagaagcc atagaggttg
     6001 ggcagaaaag tggtgctagg gttcaagtat ctcacttaag tccagttgaa atagaagcat
     6061 ttgatgagat attaaaggct aggtataatg gtgttgaaat aatggttgac acagtaccaa
     6121 gaagttctgg gcattgtatg aagaaaaaaa gagtaattca atttattatg gcaatatcat
     6181 ctagtctatt tgaattaggt atagatgggg taatggatgc tcttagaaat gaagagggaa
     6241 gaacacttat attaaaagaa gcatttatac ttggagatag ggggagtata atacttctaa
     6301 acactaaaga tattaatatg gaaaagaaat caatacgtga aatagctaca caaaaaggaa
     6361 tagatgagga caagttatta ctcgatttac ttttagatgg agacgaagaa cttatctttt
     6421 gtttaggagg aatgtataga gctgatttcc cagataaact acatgatgat aaaataattg
     6481 ataatccatt tgttatggtt ggttcagatt gtttgtttag tgtgggtggg gatatgtcat
     6541 ggtttgaact acaaagaaga ggagcttttc caattttctt caatatgtac agaaagtctg
     6601 gtgttagatt ggaagagatt gtaagaagag ttacttcatt accagctagg caatttaaaa
     6661 taaaaaatag aggtattctt aaagaagggt tgatagctga tatagcagtt attgacatgg
     6721 ataactatag ttatcctagg tcagaagata ttgatttttc aaagccacaa ttactagcca
     6781 atggtgttga atatgtaatt gtaaatggaa aaattgcgct agaaaatgga gagattactg
     6841 agaataaatg tggtgaagta ttaaaaagat aaggttttaa aaataatagg atttagaaag
     6901 taaataaata tctaagtaag taaagacgaa gtttgagatt tatttaaata aaaatcatgc
     6961 ttcgttttta tttatagtat taatatttat tttaataatc agataatatt tatactttat
     7021 agtgaaaaaa ttaatatttt taatcattaa attatgtaaa aaattggata aatagataac
     7081 taaatcattt gagaaaagat ttttagaaat aaaaaatgat aactaaataa cccctttaat
     7141 atttatcaaa tagccagtca taaatattaa aaggggttat ttagtttata aaaattattt
     7201 gtaataaagt aggatatatt aataaatatg atagaatata tattataaat taaatttatt
     7261 aatataaggg gggaactttg agttcttata gatttcttac attataagaa ccaatacttt
     7321 atgcgggagt tattaaaagt aacacttata gtaatgcttg ttttgataaa tgcttttttt
     7381 gtagcttgtg aatttgctat ggtaaaactt cgtagttcta ggattgatac catgataact
     7441 gaaggtaata ataatgcaaa aagatgtaaa ataataaaag acaacttaaa ttcctatctt
     7501 tctgcatgtc aacttggtat aacactttgt tcacttgcat taggttggat gggagaatct
     7561 acagttaaag agttaatttt accaatagta agtgtattta atcttagtga aagtattata
     7621 tatactatat caatttccat ttctttttta attataacaa tgatggaggt tgtaatagga
     7681 gagttggttc ctaaagcttt agcactttat aatacagaaa aaataatgtt gagtacatct
     7741 tttttattag taggatttta taagttaaca tatccaataa tatatttttt caacttaagt
     7801 actgatttat ttcttaaacc atttggatat tcacaagctg atgaggttga tgaaccccat
     7861 acaggtgacg aaataagact attagtagaa gaaagttata aaagtggact tatagatgaa
     7921 tcagagcaaa gattagttga taatattttt gaatttgaag aaaagaaaat tagagagata
     7981 atggttccta gaactgatat ggtatgtata tatgaaagtg attctgaaga aaagatatta
     8041 gctattttaa aagaggaagg tgttacaaga tatccagtat gcagaaaaaa taaagatgat
     8101 atattaggtt ttgtacatat aagagattta tacaatcaaa agataaatga aaataaaatt
     8161 gaactagaag aaatattgag agatattata tatatatctg aaaatttaac tatagataaa
     8221 gctcttgaga gaataagaaa ggaaaaactt caacttgcta tagttgtgga tgaatatggt
     8281 ggtacatctg gtgtggtaac aatagaagat atactagaag agatagttgg agaaattcaa
     8341 gatgaatttg acaaagaaga gactcatatt aagaaaatag gagcaggttc ttacttagtt
     8401 gatggaactg aatctatcaa ctatattaat aagtattttg gacttgatat agagcatgat
     8461 ggatttgaca gtataggtgg gtggatatcc tatatgcttg gttcaaatat taaagtaaac
     8521 caaagtgtaa cttttgaaaa ttacaatttt acaatattag aattggataa attgagggtt
     8581 gtaaaattaa tcataaaaag tgtaatataa ttatatcaaa aaatattgga ggaattgtat
     8641 gcaaaaagta gaaggaatta tttttgacat ggatggagtt ttgtttgact cagagagaat
     8701 ttctcttgaa ttttggatgg agacgtttga aaagtatgga tatactatga ctaaggaaat
     8761 ttatacatca gttatgggaa gaaatcgtaa aggaataata gaaggtctta cagatatata
     8821 cgatagttct gtaccaataa tagatttata tgacgaaaaa actaaaaata tgattgaatt
     8881 tatggagagg aagggagctc ctatcaaatt aggagtcaat gaactaatct cttttttaaa
     8941 ggaaaatgga tacaaaatgg ctgttgcaac atcaacaaag agagaaagag cagtaaagag
     9001 attagctaag gcaaatttaa aagattactt tgatgctata gtttgtggag atgatgttgt
     9061 aaactctaaa ccaaaccctg agatattctt aaaagcagct aaaaagataa atgttaatcc
     9121 ccaaaattgt attgtcatag aggattcgcc tatgggagtt gaagctgcat acaatggtgg
     9181 tataagatgt ataaatgtac cagatttaaa agagccagat gagcaaatta aatcacaatc
     9241 acataagata cttgaaaatt tactggaagt tagagaatac ctaaaaagtt tgaactctaa
     9301 agaatgtcac aatctataat ataaagtatt aattaaataa attaaatata atttctttga
     9361 tttagaataa atccatgaat atgagaaaaa ataagtataa acagctagga ggaatattca
     9421 tggataatgt aggttctcta ttatattttt tatttaattt tttcttgtat ggttttatag
     9481 gttggataat tgaaaactta tatagttatt ataaacaagg gcattttcaa gaagatggat
     9541 ttttaaatgg tccatttaaa ccaatgtatg caattgcaat gtcgattata atagaggtct
     9601 caagatttat tgaccccaat aatatatttg cattgctttt attatgtcta gtagtgccga
     9661 cagtagtaga atatattaca ggaatactta tgaaagtata ttttaataag gtctattggg
     9721 attattcaga ttataagtac aatagtagag gaatagtatg tctaaagttt tctgtatatt
     9781 ggactatatt gacatttatt ggagttagat attttcaagt gtatatagtt gatagtttat
     9841 atagtcttat aataccttat tggccaattg ttgtaactat acttttagta acattgtttg
     9901 tggatgaaat tcttactata aaatttttga ggggaaataa agatatagta gaaagataac
     9961 tttaaaaata taagttgaat ttagattgta gttaaaagaa agctatgtta aaatggtgaa
    10021 atcatttaaa attagctttc ttttttattt gataaaatta ttttataata tatctataag
    10081 ttgtaaagtt ttttattaaa attaaaataa agtgtagaaa atggggagtg atttatgatg
    10141 ggtaaaataa gatttggtat tgttgggaca agcaatatag caaatatatt tctaagagct
    10201 gcatctaggg ttaaagactt tgaattggtt gctgtatatt ctagaaattt agaaaaagct
    10261 agagaatttg gaactctata tggagcaagt attttttttg atgatttaga acaaatggct
    10321 aaatcgaagg atatagatgc tgtatacata gcttctccaa atgcattaca ttcaaagcaa
    10381 accattactt gtttaaaaca caaaaaacat gtgctttgtg aaaagtcgtt agcatctaat
    10441 ttaaaagaag tcaaatcaat gataaaaaca gccaaagata ataatgtatt gcttatggaa
    10501 gctatgagaa ttacatgtgt tccaaacttt aaagcagtta aagaaaactt atataaaata
    10561 gggaaaataa gaagattttt tggaagctat tgtcagtatt cttcaagata tgataaatat
    10621 aaaaatggaa ttatagaaaa tgcctttaaa aaagagttgt caaatggagc tttaatggat
    10681 ataggagtgt attgtataca tcctatggtg aatttatttg gtgctccaaa aactgtaaag
    10741 gcagtttctc atatacttca aactggagtg gatggagaag gttcagctgt atttcaatat
    10801 gatgatatgg atgcgataat tcagtattct aaagtagctg attcttatat accatctgaa
    10861 atacaaggag aagagggaag tataataatt gaaaagctta atctatttga aaaagctatt
    10921 attaaataca gagatggaag agaagaagat atatctattc caaaagaaaa agcagaggaa
    10981 aagcctagag aaatagaagg aatgtattat gaacttgtag agtttataag tcttataaat
    11041 aacaataaaa tagaatcaaa tataaattct catgaaaatt caataatagt aatggaaatt
    11101 atggatgaga taagaagaca aagtagtatt gtttat
//
LOCUS       DAYAOX010000121        11107 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.121, whole genome shotgun sequence.
ACCESSION   DAYAOX010000121 DAYAOX010000000
VERSION     DAYAOX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.121"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            407..1726
                     /gene="dnaA"
                     /locus_tag="ACGI57_002447"
     CDS             407..1726
                     /gene="dnaA"
                     /locus_tag="ACGI57_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFG9717522.1"
                     /translation="MDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILLA
                     PSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYPN
                     LNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHHI
                     VSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERTQ
                     EEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIAI
                     LRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEAL
                     KDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTDL
                     SLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            1968..3074
                     /gene="dnaN"
                     /locus_tag="ACGI57_002448"
     CDS             1968..3074
                     /gene="dnaN"
                     /locus_tag="ACGI57_002448"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFG9717523.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            3211..3417
                     /gene="yaaA"
                     /locus_tag="ACGI57_002449"
     CDS             3211..3417
                     /gene="yaaA"
                     /locus_tag="ACGI57_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFG9717524.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            3435..4550
                     /gene="recF"
                     /locus_tag="ACGI57_002450"
     CDS             3435..4550
                     /gene="recF"
                     /locus_tag="ACGI57_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFG9717525.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            4551..6452
                     /gene="gyrB"
                     /locus_tag="ACGI57_002451"
     CDS             4551..6452
                     /gene="gyrB"
                     /locus_tag="ACGI57_002451"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFG9717526.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            6472..8898
                     /gene="gyrA"
                     /locus_tag="ACGI57_002452"
     CDS             6472..8898
                     /gene="gyrA"
                     /locus_tag="ACGI57_002452"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFG9717527.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            8967..9263
                     /locus_tag="ACGI57_002453"
     CDS             8967..9263
                     /locus_tag="ACGI57_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFG9717528.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            9263..9523
                     /locus_tag="ACGI57_002454"
     CDS             9263..9523
                     /locus_tag="ACGI57_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFG9717529.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            9535..9852
                     /locus_tag="ACGI57_002455"
     CDS             9535..9852
                     /locus_tag="ACGI57_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HFG9717530.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            9856..10263
                     /locus_tag="ACGI57_002456"
     CDS             9856..10263
                     /locus_tag="ACGI57_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9717531.1"
                     /translation="MACETIKMEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVA
                     VSEACTNAIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLG
                     LFIIKTLMDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            10263..11036
                     /locus_tag="ACGI57_002457"
     CDS             10263..11036
                     /locus_tag="ACGI57_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HFG9717532.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
BASE COUNT         4509 a         1148 c         2021 g         3429 t
ORIGIN      
        1 gtttatattt ttatcaacaa ttagttaata ttgtggataa attagttaca aatcattgaa
       61 gtttgacata tattttgata ttataaatat acttgttgat aatgtgcata aatgttatca
      121 acttattaga aattattcac agcttgtgga tacttttgtg tataactttt aagattactg
      181 acaatactct agttataatc accatttatt gtatgtttat gttatttata actttatcta
      241 catattgttt atatcttttc atattttttt aataatttat actattatta atttatcaac
      301 accattatcc ataaatttag ttattctgta aaatttatat atatttttgc ttttctgtta
      361 ataactttgt ggaaaatatt aattattctg ttcggaggtt tatattatgg atatagtttc
      421 tttatgggac aaaaccctac aattaataaa aggtgactta acttcagtaa gttttaatac
      481 cttttttaaa aacatcgtac cattaaaaat acatcttaat gatttaatct tactggctcc
      541 tagtgatttt aataaagata tcttagagaa tagatattta catttaattg aagatgctat
      601 tagtcaactg tcattaaaaa aatataatat taaatttgta ttgagcgaaa aagaggttgc
      661 tgatttaaat tctgatagca cagatttaaa ttatagagtt ctttacccca atctaaatcc
      721 aaaatacaca tttgatacat tcgttattgg taatagtaat agatttgcac atgctgcatg
      781 cgttgcagtt gcagaatctc cagctaaagc ttataaccca ctttttttgt atggaggagt
      841 tggattaggt aagactcacc taatgcatgc tataggtcat catattgtta gtcaaaaaaa
      901 agactcaaaa gtagtttacg tttcttcaga aaaatttact aatgaactta taaactctat
      961 aaaagacgat aaaaacgaag aatttaggaa taaatataga aacgtagatg tattactaat
     1021 cgacgatatt caatttatag caggaaaaga aagaactcaa gaagagtttt tccatacatt
     1081 caatactctt catgaagcaa acaaacaaat aattatatct agtgatagac cgccaaaaga
     1141 catacctaca ctagaagata ggctaagatc gagatttgaa atgggactta taactgatat
     1201 tcaagcacct gactttgaga caagaattgc tatacttaga aaaaaggctc aactagaacg
     1261 tatagatgtg ccaaatgagg ttatgtcata tatagcaaaa aatattaagt caaacataag
     1321 ggaactagaa ggtgcattaa ctagagtggt tgcttattct tctcttagca atagggtaat
     1381 atcttttgat cttgctacag aagctttaaa agatattata acaacttcta aaaatgaaga
     1441 aataaatgtt ctcagaataa aagaaaaagt atcttctgta ttcaatttaa agatggaaga
     1501 ttttaattct aaaaaaagaa cacgttcaat agcttatcca agacagatag ctatgtattt
     1561 aacgcgcgag cttactgatt tatcccttcc taaaataggt gaagagtttg ggggtagaga
     1621 ccatacaaca gtaattcatg ctcatgacaa agtatctaag gatatagaag aaagtgaaga
     1681 aattaagact aaaatagaca aaattatatc agatttgaag ggataattta tacacaattg
     1741 tatgtttata tctcttgaat aacctgttaa tacttttttt gaacaaaaat tattaacaaa
     1801 ttatcaagtc taaaataaca aagatattaa catcttatac acatgttaat atctttgtat
     1861 ttataatgtt atacatactt attaacatta tcaacaacac ctactactat tactactatg
     1921 attttaactt atttttatat ataaaaagcc agaggaggtt atccacattg aaaataattt
     1981 gtaatcaaaa aatcctagca aatagaattg gaattgctca aaaagctatt aatggaaaaa
     2041 caacaataga attattaaaa ggtatactta tatctacaga agaaggtcaa ttaaaactta
     2101 ctggatatga tgctgaaata ggaatagaaa cttatgtaca agcagaaatc atagaaaaag
     2161 gtgatgttgt tgtagatgca aggctatttg gagatataat tagaaaatta ccagattcct
     2221 ttgttgagat agaaactgat tcagaaaata atatttacat aaattgtgta aattcaagat
     2281 ttaaaattaa aggttatgca gcaaaagaat ttcctaaatt accagaatta aatgaagaag
     2341 atttatatag catacctcaa gaaatcctaa aaaatatgat taaacaaact gtatttgcta
     2401 tctcacagga ccaaacaaaa cctgttttaa tgggagaact tttagaaatt gtagatagaa
     2461 acttaaattt agtagcaatt gatggatata gattagctgt aaaaagttgt tcggttgata
     2521 gtttaactga aaatataaaa gtcataattc ctggaaagac acttatagat gtaaatagtt
     2581 tgctttctgg agaggataat gttaaagttg gatttaatga aaaaaatgct atttttataa
     2641 ttaatgatac gaaaattatt acaagattgc ttgaaggaga ttttattgat tataaaaaat
     2701 tattgccaag agaacataac agcagagtta aattaaatac taaggaactt ttaaatagta
     2761 tagagagagc gtctttattg tctcaatcag aaaaaaataa tcttataaag ttatctataa
     2821 gagataaagt aatggcaatt acttctaata cagaaaaagg aaatgtgtat gaagaagttg
     2881 aaatagattt agatggagat tacctagata tagctttcaa ttctagatat tttatcgaag
     2941 gtttgaaaaa tatagataat gaggaaatat ttatagaatt tactacaaat gtaaatcctt
     3001 gtataattaa accaacagat gatgttaatt atatttactt actgcttcca gtaagaatat
     3061 catcaaatat atagcagaca tgaaggagtt gtcttttgta gaaaattttt ctaatcttag
     3121 acaactcctt ttataagcat atttatggta taatatactt ttagtgtatc aataggaatt
     3181 tgtttaaaat agtgacaagg aggaactatt atgactgaaa taactataga atcagaatat
     3241 ataaagttgg atcaatttct taaattagca gagattgcat caacaggagg tcatgcaaaa
     3301 tttttaattc aagaaggttt agtgacagta aatgatgaaa tagaattaag aagaggaaaa
     3361 aagataaaat caggagatat agttgaaata gaaggaacta aaataaaagt attgtaaaaa
     3421 aaggagaact ttttgtgaaa cttaaaagtt tgcaacttgt taattttaga aattataaaa
     3481 agttgcatct ggaatttaat gggaaagtaa atctacttgt tggtaaaaat ggtcaaggaa
     3541 agacaaatat agtagaatct atttatatgc tttcttttgg aaaatcattt aggactaaca
     3601 aagataaaga aatggttaga tttaatagtg aaaacttata tattggagga agtttttcaa
     3661 aatataataa atatagtcta atagagctaa ttattggaaa agataaaaaa gggataagaa
     3721 taaataaagt tcctttacaa aagatacaag aactacttgg caacttaaat gttgttatat
     3781 tttctcctga ggatttaagg ttggttaaag agggtcctaa agaaagaagg gcatttatag
     3841 acaaagagat tagtcagatt atacctaaat attataaata tcttaccaat tataataaaa
     3901 cactttctca aaggagtagg gtgcttaaaa atatacatgt tgatgaagct ttattagatg
     3961 tatatgatga tacgttagca aagtatggaa gttatattta tattttgcgt agagacttta
     4021 taaaaaagat agctaatata tctgaaaata tgcatatgaa tttgactaat ggagttgaaa
     4081 gattatcaat tcgatataaa aatcaaataa atataactga tgaagatact atagatacag
     4141 tatataataa atttttagcc aagttgtcat cgaatagacc taatgatata gaatctaaga
     4201 ctaccagata tggtatacac aaagatgact taaatatatt cataaatgat ttagatgcaa
     4261 gactttttgg ttcacaaggt caacaaagaa ctgcttcaat atcattaaag ttatcagaga
     4321 tagaattaat aaaaaatgaa gttgaagaat atccagtatt aattttagat gatgttttta
     4381 gtgagttaga tgaagcaaga caaaaacttt tagtaaataa tttaagcaat gttcaaatgt
     4441 ttataacaag tgctgaagtt tcacataaaa aaatatttga tgaaaaaaat gttacaatat
     4501 ttaatattga aaatggcgat gttattagca tagagaatgg aggaaattaa atgaaacaag
     4561 aatacggtgc aagtcagata caagtattag agggattaga agccgttaga aaaagaccag
     4621 gtatgtatat tggtagtaca agccctagag gtttacatca cttagtttat gaagttgtag
     4681 ataatagtat agatgaggct ttacaaggat attgttcaga catatatgta tctattaatg
     4741 aagatggcag tgttttagta aaagataacg gaagaggtat acctgttgaa atacatccta
     4801 aaactggtaa atcaacttta gaaacagtat taactaatct tcatgcagga ggaaagtttg
     4861 gaggcggagg atataaagta tctggaggtc ttcatggagt tggtgtttct gtagttaatg
     4921 cattgtcaaa atggatggta gcagaggttt atttaaacgg aaagatatat aagcagacat
     4981 atgagaaagg attgccaaca tctaagcttg aagtagttgg agaatctcaa gataaaggaa
     5041 caatgattca atttatgcca gatgagacaa tatttgatga aatagagttt aaatatgaaa
     5101 cattggagta taggcttaga gaactttctt tcttgaataa aggaataaaa atagtatttg
     5161 aagataaaag agaaggacaa gaaaaaagaa aagaatttca ctatacaggt ggtttggtag
     5221 aatatattaa gtatttaaat aaatctagaa ctggtataca tgatgacata gtttatatag
     5281 ataaaaaggt tgatgattgt tttgttgaac tagctatgca atatacagat ggttatacag
     5341 agaacatata ttcttttgca aacaatataa atacacatga aggtggttct catttaagtg
     5401 gatttaaagc agctcttact aaaactgtaa atgattatgc taagagaaat aaatttttaa
     5461 aggaaaatga tgttaaccta cttggtgaag acataagaga aggtcttaca gcagtagtat
     5521 cagttaaatt accagaacct caatttgaag gtcaaactaa aacaaagcta ggtaatagct
     5581 ttatgagagg tatagttgat agtgtaacag ttgatgaact ggggtctttc ctagaagaaa
     5641 acccatcaac agcaagaatc atagttgata aagctttaag agcacaaaga gctagagaag
     5701 ctgcaaaaaa agcaagagaa ttaacaagga gaaaaagtgt attagaaagt acatctttac
     5761 ctggaaaact tgcagattgt gcagaaaaag atccatctaa aagtgaaata ttcttagtcg
     5821 aaggggattc agcgggaggt tcagctaaac aaggtagaga tagaaatagt caagctatac
     5881 ttccattaag agggaaaata cttaatgttg agaaatcaag actagatagg atactatctt
     5941 cagatgaaat aaaaaatatg ataacagctt atggttgtgg tattggagaa gattttgata
     6001 tagataaggc taggtatcat aaaattataa ttatgaccga tgctgatgta gacggagccc
     6061 atataagaac tttattatta acattcttct ttagatatat gagacctctt atagatgagg
     6121 ggtatgttta tgcagcacag ccacctttat ataaagtgac aaagcaaaag aaggaacatt
     6181 atgtttactc agataaagag ttaaatatct tactagatga aattggaaga aatggtgttg
     6241 aacttcaaag gtacaaaggt cttggagaga tgaatgcaga gcaattatgg gaaacaacta
     6301 tgaatcctga gacaagaact ttattacaag ttactgtaga agatgctgca attgcagatg
     6361 aagtattttc aatgcttatg ggtgataagg ttgctccaag aaaagaattt atagaagaaa
     6421 atgcaagatt tgttagaaac ttagatatat agagagaggg gattagtata catggaagaa
     6481 aataacaaaa tactccctat tgaaatagcg gaagaaatga aaaaatcgta tattgattat
     6541 tcaatgagtg ttatagctgg acgtgctctt cctgatgtta gagatggttt aaagccagtt
     6601 catagaagaa tattatattc aatgagtgag ttaaatttaa ctccagataa accatacagg
     6661 aagtcagctc gtattgttgg ggacgtttta ggtaagtacc atcctcatgg agatactgct
     6721 gtttattatg ctatggtaag aatggcacaa gatttttcaa ctagagcact tttagtagat
     6781 ggtcatggta actttggttc tgttgatggg gattcaccag ctgctatgcg ttatacagaa
     6841 gctaaaatga gtaaattatc attagaacta ctgagagata ttgaaaagga aactgtagac
     6901 tttaaaccaa actttgatga gtcgttaaaa gagccttcag tattgccagc tagatatcct
     6961 aatttattag taaatggctc aaatggtata gctgttggta tggcaacttc aatacctcca
     7021 cataatttag cagaagtaat tgatgcaact gtatatttga tagataatcc agagtgtagt
     7081 gtagatgatt taataaaatt tgttcaagga ccagatttcc ctacagctgc aattataatg
     7141 ggaaaagaaa gtatagcaga agcatacaga actggaagag gaaaagttaa agttaggtct
     7201 agagctttta tagaagagct accaaaagga aaacagcaaa taatagttac agaaatacct
     7261 tatcaagtaa ataaggctaa actggttgaa agaatagcag agttagttaa agaaaagaga
     7321 atagaaggta tatcagacct aagagacgaa agtaatagaa atggtatgag aattgttata
     7381 gaattaaaga gggatgctaa tgctaatata gtattaaata atttgtataa acattctcaa
     7441 atggaagata cttttagtat aataatgctt gcacttgtag atggtcagcc aagagtttta
     7501 aatcttaaac aaatattata tcattatatt aaacatcaag aagatgttgt tacaagaaga
     7561 actaaatttg aactaaataa agctgaagca agagcacata ttttagaagg attaaagatt
     7621 gctttagata atatagatgc tgttataagc ttgataagag cttcaaagac tgggcaagaa
     7681 gctaagctag gtttaataga aaaattcaaa ttaactgaaa tccaagcaca agctatatta
     7741 gatatgagac ttcaaagact tacaggttta gaaagagata agatagaagc tgaatatgaa
     7801 gatttaatca agaagataaa tagattaaaa gagattttag ctgatgaaag attactttta
     7861 aatgtaataa aggatgaaat tacaataata aaagaaaatt actctgatga gagaagaaca
     7921 gaaataagac atgctgaagg cgaaatagat atgagagatc ttataagtga tgaagaaata
     7981 gcaataactc ttactcactt tggatatata aaaaggcttc catctgatac ttataagagt
     8041 caaaaaaggg gtggaagagg tatttcagca cttacaacta gagaagaaga ttttgtaagg
     8101 cacttggtaa ctacaactac tcatagtaga ctattattct ttacaaataa aggtagagta
     8161 tttaaattaa atgcatatga aataccagaa ggtaagagac aggctaaagg tactgctata
     8221 gtgaatttac ttcaattgtc agcagatgag aaaattgcta ctctaatacc tattgatggt
     8281 aatgatgaaa atgaatattt attacttgca acgaaaaaag gtattgttaa aaagactaag
     8341 agagaagagt tcaaaaatat aaataaatct ggtcttattg caataggttt aagagatgat
     8401 gatgagctta ttggagtaga acttacagat ggaaaacaag aagtactttt agtaactaaa
     8461 gaaggtatgt ctataagatt tgatgaaaat gatataagat atatgggtag aacagcaatg
     8521 ggtgtaaaag gtataacttt aagtaaagaa gattttgttg tgtctatgaa cctttgcagt
     8581 aaaggtacag atgtgttagt tgtaagtaag aatggttttg gaaagagaac aaatatagaa
     8641 gagtatagaa gtcaaataag agctggtaaa ggaattaaaa cttataatat atctgaaaaa
     8701 actggtacaa ttgtaggtgc agatatggtc aacgaagatg atgagataat gattataaat
     8761 tctgatggag ttcttattag aataagagtc aatgaaatat cactgtttgg aagagttaca
     8821 agtggtgtta aattaatgaa gacgaatgat gaagtcaatg tagtttcgat tgccaaaata
     8881 aatattgaag aagaatagga taaatatcct attctttttg tataatgtag ttagataaaa
     8941 atttaaaatt gaaaggagta attgttatgt gcaataaaag aagttgtaaa aaaggtggat
     9001 ttttattatt aggagctatt ttagggttta tatttggtat gttttttgca cctaaaaaag
     9061 gttctgaact aagaaaagaa acaaaagaga agtttaatga tgtcaaagaa aatccaaaag
     9121 aagttctaca tgaaacattt aatgatgtta aagagagaat aataaattta gtggatgatg
     9181 ataataatga agaagatata aagatttcag aagaagatat agtaataagt aaaagctttg
     9241 atgatgaggg agatgttaac taatgaatgc atgggggtgg caaattggag cagtgttagt
     9301 aggaagctct gctcttatag tagcaattta tttagctaaa actctaaaca gcataaataa
     9361 ggttgttgaa aaggcatata agattgtaga ttataatgaa agacatatac aagatattgt
     9421 tgaaaatgca tcatcaatat caaagggaat agatgatata gtttatgtaa ttaataaaat
     9481 attgagtata ggaaatatat ttaaaattat taaaaggaaa taggaggttt ttacatgtct
     9541 atgaatattg attcaaattt agattcacaa aataaatttt ggaatgtttg tcttgatgga
     9601 gaacttgatg tttctacagc agataaacta aaggaacatt tacatgcttt aatagaaaaa
     9661 aatatgttag atgtaaagat taatcttaaa gatttagatt atatagactc aacaggctta
     9721 ggagctatga taggtgtatt aaaaaaatta aaaataaatg aaaaagagat atacatagta
     9781 aatcctaaga gtaacgtaag aaagattttt actataacag gtctagataa aatatttaaa
     9841 gtggagggat aaactttggc ttgtgagact ataaagatgg aaattactac aaatccagat
     9901 tatgttagta taataagatt aacagcatct ggaattgcca ataagatggg ttttccaata
     9961 gatgatatag aagatataaa ggtagcagta tctgaagcat gtacaaatgc aataaaacac
    10021 agtaaagatg atgtgtttaa tatagtattt aatattttag ataatgcaat taatatagaa
    10081 attcaagata atggaaaagg atatgatgtt agctcaataa gtacaccaga tttagagaat
    10141 ccaaaagaga gtggtcttgg attgtttata attaaaactt taatggatga tgtagatatt
    10201 gaatcagaac ataatcaagg gacaaaaata aaaatgacta aatatttagg agttgatatt
    10261 taatgaaaaa tgtagctaat gctacacatt acctaaatat ggatacaaaa gagctattta
    10321 atttatataa taaaaataaa aatgtagaca ttagaaatat tcttattgaa agacatctgt
    10381 atttagcaag attgttagct aagaagtata taaacaaagg tgttgatttt gaagatatat
    10441 atcaagttgc ttcattggca ttaatttatg ctattgatag atatgatgtt gagaaaggat
    10501 ttgagttctc cagctttgca acaccaacta tagtaggaga aataaaaaag tattttagag
    10561 ataaggtatg gacacttaga gtcccaagaa gaatacagga attaagcaaa aagataagtg
    10621 atgcgaagat taaattagaa caagaaaata agaagcatcc aaaggtaaaa gatatagctg
    10681 attatatagg cgttagtgaa gaagatgttt tagaggctat ggaggcttct tatggatatc
    10741 aaccaatgtc tttggattca tcaagtaatg atgattcaga ggataaagat ataactctta
    10801 ttgataagat tggaaaagaa gaaggtaact ttggaagtat agagtatgaa gattttatta
    10861 ataagtttat tgaaacttta aatgaattag aagttaaaat ttttaaagat agatttttct
    10921 ttgataaaac acaatctagt atagctaaag aactagggat atcacagatg actgtttcta
    10981 gactagagaa aaaggtagta gagaagctga aaaaagaata tgaaaaaaat ttataaaaaa
    11041 atataaaaaa agtattgacc tactgtttta aagatggtat agtattactt gtccttgaaa
    11101 aaaggaa
//
LOCUS       DAYAOX010000122        11089 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.122, whole genome shotgun sequence.
ACCESSION   DAYAOX010000122 DAYAOX010000000
VERSION     DAYAOX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11089
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.122"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            271..1215
                     /locus_tag="ACGI57_002458"
     CDS             271..1215
                     /locus_tag="ACGI57_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HFG9717533.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            1384..2316
                     /locus_tag="ACGI57_002459"
     CDS             1384..2316
                     /locus_tag="ACGI57_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFG9717534.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            2421..2888
                     /locus_tag="ACGI57_002460"
     CDS             2421..2888
                     /locus_tag="ACGI57_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HFG9717535.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(3132..4004)
                     /locus_tag="ACGI57_002461"
     CDS             complement(3132..4004)
                     /locus_tag="ACGI57_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFG9717536.1"
                     /translation="MKGNSLMLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSIS
                     AFIFSTIITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQ
                     LTFTTLLAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVL
                     LSSLFLSLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLIT
                     GILWSIANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGI
                     ILIMSGVFMLSIIK"
     gene            4152..6116
                     /locus_tag="ACGI57_002462"
     CDS             4152..6116
                     /locus_tag="ACGI57_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9717537.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            6116..6577
                     /locus_tag="ACGI57_002463"
     CDS             6116..6577
                     /locus_tag="ACGI57_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9717538.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            6580..6900
                     /locus_tag="ACGI57_002464"
     CDS             6580..6900
                     /locus_tag="ACGI57_002464"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFG9717539.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            6916..8016
                     /locus_tag="ACGI57_002465"
     CDS             6916..8016
                     /locus_tag="ACGI57_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFG9717540.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            8136..10736
                     /locus_tag="ACGI57_002466"
     CDS             8136..10736
                     /locus_tag="ACGI57_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFG9717541.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLNKSNDAFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTIFTKGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNKMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         4404 a         1198 c         1857 g         3630 t
ORIGIN      
        1 tagtattaaa tttgttaaaa agtaaaattg tagggtgctt gaaaatagca ccttttttag
       61 tttcattata aaattagttt tgaaagttta ggaataatgt atatttattt ttaaagaatt
      121 ataaccttat taaggatatt ttaaatgcta gaaatgaatc agtaaatcat actggaatta
      181 tcttaaatgt tttgattaaa ttaagtattt agagctataa ttatattaaa aatatattag
      241 aacataatta tattactatg gaggttaaat atgatagtaa gtgtgagtag aagaactgat
      301 atacctgctt tctatagtaa gtggtttttt aacagacttg aagaaggttt tgtttatgta
      361 attaatccaa tgaatccaaa acaggttagt aagatagagt taaatccaca tactgttgat
      421 tgttttgtat tttggacaaa ggatgtaact ccaatgttat atgatttaaa taaattaaga
      481 gactataaat attattttca ctatactata acatcatatg gaaaagaagt agagacatgt
      541 attttagata aaagaaaggt aatagattca tttaaggaat tgtctagaaa aataggcaaa
      601 gaaagagtaa ttttaagata cgacccgatt ttcttaagtg aaaagtacac agtagattat
      661 catatcaaag catttgcgag tttatgtaat cagcttgatg gatttacaga aaaatgtata
      721 ataagtttta tcgatttgta taagaaaaca aaatttaata ctaaagcatt acatataaag
      781 ccaatagaaa ttacagaaat agaaacatta tcaaaagaga tttctaagat tgctcataag
      841 catgggataa tacttgaaat gtgttctgaa gaatataatt taagtaaatt tgggattgga
      901 aaaagtaagt gtattgatga tagattaata tcaaaaatta taggatgtga agtagaggta
      961 aaaaaagata gtacacaaag agatatttgt ggatgtgtta aaagtgtcga tataggacaa
     1021 tacaatactt gcagacatta ttgtctgtat tgttatgcta actttaatta tgcaaaagta
     1081 gaagaaaact gtaggcttta taaggaagat aataaattac tcatagatac tttaagagat
     1141 gatgcaaaaa taagcattag agatatgaag actattaaag tagataagaa taaacaaatt
     1201 agtatatttg attaaaataa atatactaat taccttaata tattaagaat atagatactt
     1261 ttgataacaa aattaaactt aatagatatt ttctatataa gaaagatggt cataatatgt
     1321 caatttgacc atctttttga tataattaaa acacaaataa tttagtaaca aaggaggcag
     1381 aacttgaatt tattaaaaaa atatcctata cctattgcaa gtttgatttt atcaattttt
     1441 actttaggta acttattaca atcttatagt gaatcattac gtaatgtaat tggatttata
     1501 ggatttattt tgtatgcaat ttatatagtc aaaataatat tacttagaaa gggtgttaaa
     1561 gagcaattag agaacccttt aatatcaagt acatttccaa ctataacaat ggcaacaatg
     1621 atatttgcta catatataaa accattatct ttagaattaa gtattatgat ttggagcata
     1681 ggaattgtgg gtcatcttgt gttaatagtc ttatttagta agaagttctt agttaaattc
     1741 tcaataaaaa cggtatttcc atcttggtat atagtatatg ttgggatagt tgcagctagt
     1801 gttacggcac cagcatttaa tcaacttttg attggcaaaa ttgccttttg gataggattt
     1861 gtatcttata tagttcttat accagtaatt ttaaagagag tatggataat caaggaaatg
     1921 cctgaaccat ccttaccaag tatagctata ttagctgcac cagggtcact attattgtct
     1981 ggatatatta attctttttc agataaaaat aatgtagtat tatacttgtt attcatatta
     2041 tcaattgtat tttatattat agttttggga tatttaccga aattattaag gttgcctttt
     2101 tatccgagtt ttgctgcatt tacattccct atggcaataa gtgcaatggg tatgaaacta
     2161 atgaatggtt atttaactaa atcaggtcat tttataagtt ggatttcata tgcttctaag
     2221 ttagaagaga taattgctac agtgattatt ttatatgttt tagttttgtt tattatatat
     2281 cttagtaaat ctgaaactac atctaaagct aaataagaat tattaaatat gtgtatttaa
     2341 taggaatatt gatataaata tatgaagata agataaacca tagaaactta atttagctta
     2401 attttattgg aggaaaacaa atgaaaacaa aagaagaata tttaaaagaa gttagaaaca
     2461 tatcagaaga atactttaga aaaggagagt ttttttgctc tgaagcagtt ctccagacaa
     2521 taaatgatgc tttaggccaa cctttaagtc cagagataac taagttagca tcaggatttc
     2581 cgataggact tggaaaggca caatgtcttt gtggagcaat atctggggga gagatggctt
     2641 taggtatagt atatggaaga gttcatggtg aagctatgaa tccaaagatg tttgagcatg
     2701 ctaaaggttt gcatgattat attaaaaaag agtatggtgc aacatgttgt agagctatta
     2761 caaagaaatg ggatggtgat aactttatgt ctcccgaaag aaaagagcac tgtattaaaa
     2821 taacaggtca agttacagag tgggttgcag ataagcttat agaagatgaa aaacttaata
     2881 tcaaatagtg taatattagt tggtgaattt ataaagttaa atttacttat agtataagat
     2941 acttgatatt ctcgtattga tagaaaaatt tatagcatat gtatttaatg aaatatactt
     3001 atatttagag tgtggtttaa tttcataaat acatatgcta ttattttaag tatataagca
     3061 aatttattta aataaataga aacagttaat ttatatgtac aaaactatat atgattagtg
     3121 taaataaata cctattttat aatactcaac ataaagacac cagacatgat tagtattata
     3181 ccaataaaaa tagcaagcca ttcttttttg ttcttctttt ctttaaaaaa tataatccct
     3241 cctaaagtgg ctactatcac acaactctga gagatagaaa aacttattgt aactcccaat
     3301 gtattagtcg ttatgaacat acctaaattt gctattgacc acaatatacc agtaattaaa
     3361 ttgaataaaa cattttccct atatacaatc tctttagaaa aatacttgac aataatagat
     3421 gaagttatca acataccaac agattgaggt aatataattc tatatccttc aattaaaaat
     3481 atttgattag ttacaacata taatgataaa aatagagatg ataataatac aactccaaca
     3541 cattttaaca tatttttatc tgtagatttt ttagtttgat aatttgtgag aagtatacca
     3601 agtattatac ataatattga taaagaacca atcagtaaca ttttagaact ggtccattca
     3661 ttaaacaaga ttacagctaa taatgtcgta aaagttaatt gtgaaccatt tgaaattggc
     3721 attactttag atacactagc aatttgaatt gctttaaact gcattaattg tcccaaactc
     3781 cagaatatgc cagaaatcat acttatacaa aatgaaaaaa atgtaatttc aggatgtata
     3841 accagagtta ttatagtgga aaatataaac gcacttatgg atgtacctaa aagctgttca
     3901 tgaggagaac ttttctttag atttgcaact atgggcatca atccccatcc cattacagga
     3961 agtaaagcaa taattattgt cagcattaaa ctatttcctt tcatattaaa tttagacagc
     4021 ttttaattta tcatctaaaa ttaaagtgtt caatacataa aaattaccgt atttaatcag
     4081 gaaatattag tagtttttta gaaaaacatt ttccattaaa ataaagacaa agggaaggag
     4141 taaaattgaa aatgttaact gatatgattc aaaatcgtga aattaaaata ataaagtttt
     4201 tattaaaaga aggtcataca actgtaaaag caatagcaga tgatttagat gtttcagaaa
     4261 aaacagtttc aaaatctctt aaagaagtag aagcagtgct taaaaagata ggtctttctt
     4321 taataagaaa acctaagata ggtgtttata ttgatggaga tttaaaaaaa atacttcctt
     4381 acctagataa taaaggaagt caaattccta caaccagaga ggagagagtt atctatatat
     4441 attcagtact tttaaagagt aatgattata ttactataca acaactttct gatgaactat
     4501 atataagtcg tggaactatt gaaagggatt tagcagaagt tgaaaaactg ctaaaaaacg
     4561 aagggatttc catatataag aaacctagta agggaataaa gttaaatata agtgaaagag
     4621 ataaaagaca acttactgct aagtttatac ataaattttg gagtagaaac tggtatatta
     4681 agcaagagca agaaagcttt tatcaagcat ttgaaaagat acaagcagat gtaaatggta
     4741 ttttttcgga agatgattta tcaataatta ttgatattct taggaatttc agcagagaaa
     4801 gaaaatttga gttctctgat tatgaatttc agtcattagt tattcatttg gcaatagctg
     4861 tagagagaat tagaaaaggc gaatatatag aagaaagttt gtatactggt aatatagaaa
     4921 ataatcaaat gtcaaatacc aattatttag caaaacaatt agaagaaaaa ttttcaatat
     4981 tattaccaaa atctgaaatt ggatatataa atttacattt agtagcgtct gaccaacata
     5041 gagatactaa tgaactaaaa aatacttcaa ctaatccttg tgagacaagt acaaataata
     5101 tatcaaatct tcgggaaatt ataaaaaata cattgtatga gacagcttat gacaaggatt
     5161 taattgatgg attaacttca catgtacatt catcaataaa tagactgaaa tatggactta
     5221 gtattaaaaa tccatatgta aatacaatca aacaaaactt ttctttatca tttgagcaag
     5281 ctttagattt aaagaaagta attgaattta tatatgatat taggataaat gatgatgaat
     5341 gtgcttatat agcattacat tttgaagctt ttagagaaag aatgaaagaa cttccacaaa
     5401 atattagagt aattctagta tgcagtacag gattaggttc atctaggtta ttgtctgcaa
     5461 gaataaaaaa atactttcca atgataaaga ttgaaaaagt actttccgta caagaattag
     5521 tatccagtaa aattgatgca gatttagtaa taagtactat ttatttagag ttagaaacaa
     5581 ttccaaccat agtagttagt cctattttaa atgaaagtga tataagaacc ttggaaagaa
     5641 atatacaaaa tgtttcaagt tatacttcta agaagtataa atctttcaga aacttaatct
     5701 ttgaagaaaa tatattgttg aatattgaag cttcaaacta tgaagatgca attaagtata
     5761 taacagataa cctaataaaa aaaggttttg caaaagaagg tatagctaaa agtgcaattg
     5821 agagagaaaa tttatcttac acttctttcc aatcaatagc tacacctcat gctaatccta
     5881 gttatattaa aaaatctaat atatctgttg caacgttaaa aaatcctatt acatggggaa
     5941 atgaaaaggt ggaagttatt ttctttattt cattagaaaa tgacaaaaac ctagagcttg
     6001 atgaaatata tgaagttttt tatgacttca ttgacaataa aaaaaatata aatgacatat
     6061 taagtgcaac ttccacaaag gaagtgtacg atttgctagt ggaggagagt atatagtgaa
     6121 aatatcagat gtattgaaaa aagagcaaat tgtttttaat cttgatacaa atacaaagga
     6181 agaaacaata aaaaaattaa gtagtatatt tgttgaaaat gggattgtgg acaatgaaga
     6241 agaatatgtt aagtctgttt tggaaagaga agaacattca actacaggta ttggtaatgg
     6301 tattgctatt cctcatggga aaagtaatgc tgtcaaaaaa tcttctataa tatttgcaaa
     6361 gactaagcat aagattgaat gggatgcttt agatgaaaaa ccagtagata gtattttctt
     6421 attggctata agcaatattg ataaggatag caatcatctt gtactattat ctaaattagc
     6481 aactaaactt atggatgatg acaatgtaga tgcactaaaa ttagccaatt cagaacaaga
     6541 aataatcaat atatttagtg aagaaggaga gatgtagaaa tgaaaagaaa aattatagca
     6601 gttacagcat gtgcaacagg agttgcacat acatatatgg cagctcaagc gttaaagaaa
     6661 gcttcaaaaa agatgggtca ccttataaaa gttgaaacac aaggagctac aggaatcgat
     6721 aatgagttaa ctacaaagga tgcacaaatt gcagaagtag taatatttgc agtagatact
     6781 aaagtcagaa atgctgaacg ttttgaggga aaagaaatac tagaagtacc agttaatgca
     6841 cctataaagg atgcagagaa agtaataaat gatgcactaa agttaattga cactaaataa
     6901 atggagggaa atgtaatgtt gaaaaagata gtctctgagt taaaaaagca tgtcttaact
     6961 ggaatctcat atatgattcc attagtaatt gcaggtgcta tgataatggc gatatctcgt
     7021 gtaggtggtt ctttctataa tataccagat atatgggatg ctaaatatgc agaaagtgct
     7081 agtagtattg ttagattatt acatgattta gatgggtttg gaggtactgc attaggatta
     7141 atgtttcctg taattgctgc atttatagga ttttctatag ctgataaatt agctattgta
     7201 ccaggtcttg ttggaggtat gattgctaaa gatataggtg caggattttt aggagctttg
     7261 gcagttggtc ttatagcagg atatacatgt ttgtttatta aaaatcatgt taaattacca
     7321 aaatcagcag cttcaatagt accaatattt atagtaccag tatttgggac attaatcact
     7381 gttgtattaa tcaattatgt gataggtata ccttttgcca cattaaatac tggattagaa
     7441 aattggttaa atggattatc tggtacaaat cagatactaa tggcagctat tataggggct
     7501 atgatggcag ttgacttagg tggaccagtt aataaagcag ctttaacaac atcacttgca
     7561 ttattaacaa gtggaattta tgcgcctaac actgctgcaa tggtaggaat agttatacca
     7621 ccactaggat tgggacttgc tactatacta gcaaaacaaa aatataataa acagttacgt
     7681 gaagctggaa aatcatctct tataatgggt ttaattggag taagtgaagg agcaattcct
     7741 tttgcggtag aatctccatt aaaagtaata ccttcatgtg taattggaac agcaattgct
     7801 tcagctatgg cagttggttt aggttcagta aatgcaactc caattagtgg attttatgga
     7861 tggtttacag tagagaattg gccaatgtat gtgttatcaa tagcagttgg aacagtaatt
     7921 gtagctggat tagttatagt attgcgtgga aatccagtga ttgaggatga tgaatttgaa
     7981 gatgatgatt ttgatgaagc agagtgggaa tcttaaaatt taataactat ttaagacgtg
     8041 ttataagcta tattcaatat gaatcttata ctaaataaag tgttaaacat tcagtttaga
     8101 cttaaatata aaagataaag aacagaggag aataaatgat aaaagcacat attgttaatc
     8161 atacacactg ggatagagaa tggtatttta cttctggaga tgcattagta cttagtgaac
     8221 aactatttac agatgtaatt gatgaattag aacgtaatcc agatgtaagt tttgtacttg
     8281 atggacaact ttctattttg gatgattatg tacaattaca caaagaaaaa atagaaatca
     8341 ttaaaaagtt aataaaagaa gatagactac atattggacc atggtttact caaactgatg
     8401 cattctttgc tagaggagag tctattttaa gaaacttaat gattggaatt tttgaaagta
     8461 aaaagtatgg taagtatatg aaaattgggt atttgccaga tacatttgga tttaatgcac
     8521 agatgcctat actattaaag catgtaggtc ttgataatat attcttttgg agaggtatac
     8581 atcttggtaa acaggttcaa tcaccatatt ttaagtggaa aagcttaaat ggagaaagtt
     8641 atatatatgc agtaaatatg ccacatggat atggaactgg tatgctttta gagccttcat
     8701 taaagtatgt taatggacgc ttagacccag ctattgattt tattaaatca tatactgatg
     8761 tagatgaggt gttaatacct tctgggaatg accagttaaa tataataggt gatttcaaaa
     8821 ataaaattaa agcgattaat gatatcggaa agtattcata tataacgagt tcatatcaag
     8881 agtttttaaa atatataaag tccatagaat cactagaaag ctatagagga gaatttagag
     8941 aacctgtatt agcaagaata cataaatcaa ttggttctag tagaatggat ataaaattag
     9001 catgtgataa actagaaaac aagctgatta aaagaataga gccattatta gtaattgcta
     9061 aaaagagtaa aattgaaata agcaatcagt tacttataaa tacatggaaa aaacttttgg
     9121 aaggacaagc acacgatagt ttagctggat gtgtaactga tactgtaaca gatgatattc
     9181 ttcaccgtat aagagaagca aatgaaatat gtgacagtat tgaaaatact attgtaaaaa
     9241 agatttcaga agggcttaaa ttatctaaaa atgatatttt agtatttaat acagaagcta
     9301 agcgttttaa tggatataaa gagatacaag ttgtaagtga tagtaaaaat atttatttta
     9361 aagataattt agatgctact ataattgaag aaacttatgt aaaaccgaga gaaaatgtat
     9421 tagaagaaac acctgctggt aatatattta ttgaagaacc aggatattat atattaaaag
     9481 taagaataaa tgtacagctt cctgcacttg gttataaagt tgttagtttt gaatcaagtg
     9541 ataaagaaat ggtgtcactt aataaatcta atgatgcttt tatatctaat gataattata
     9601 agataatata tgaagatgga agcttaaatt tacaacttaa agatggaact tacatatcaa
     9661 actttttaac attaaaagat agtggtaatg caggtgatac ctatgacttt tctccactaa
     9721 aaaatgatga ggatataaaa ctttcgtttg ataaagtgta tacagaaaaa gctttaggat
     9781 atgaaaaaat ggtggttaaa ggtactactt tactaccact tacgttagaa gatagaaaaa
     9841 acaaatatct taatgggaaa ttaaatgtaa aagtaagtat tgtcttatcc aaagaaaatc
     9901 cattgatgga tgtaaaactt tgtgttgata atacgatata taatcataga ctacgtgttc
     9961 atataaaaac tgatataaaa gataataaaa acatagcatc attgccattt ggatatataa
    10021 caagagaaaa tggagtttta gacaactggg aagatattta cagtgaaatg cctatagatt
    10081 tagaaccttt ggaaagtaat attactctaa caaaccaaaa tagaagctgt actatattta
    10141 ctaaaggaat aaaagagtat caacatattg aagatgaaat tgcattgacc ttacttgcta
    10201 ctacagatga actaggaaag cctgatttat tatatagacc aggacgtgct tctggtgata
    10261 caactaaaaa aggtcacata agaattaaaa cagataaagc tcaaatgctt aagaaattag
    10321 aattttcatt tgcaatatat atggattcta aatcatttga tgaattggaa attgcgaatt
    10381 taacccacaa ttatctaagg gaatgtgtaa attatcaatt acaggattac aatttctttt
    10441 tatatcgtat agataataag attcaaaaat caattgtcaa taaaactgta agtagagaat
    10501 tagaaataat atctttacca gaaaactatc ttatatcagc atgttatcct tcttattaca
    10561 gtaaagataa atttataata agagtggaaa atccaagtag taataaaatg atattggatg
    10621 agcaaatatt taaaaataga aatgggaaaa ttgtaaatgc aatagaagat gtagaagagg
    10681 aacaggtata tgaaatagca ccttttgacg ttatttcaat attattggat ttataagtgt
    10741 aaaagtaagt ttattgttta aagtctgaca gatattaatc tgccagactt tttaatttgt
    10801 ataaatttgg gtaaatatta atgaatggtt ttagtattta agtatgaatt tagtatttaa
    10861 tatagaattt attgaagtag aataattagt ttaacaggat aagcagttta aataaagaat
    10921 tattatatta gagtgatttt atatataatt taagataata aaattttaat aaaatgagtt
    10981 aaagattgta tattaataga gttgatgtag ttacaaaaaa gttaccaatt gttatgtatt
    11041 tacaaaactg atatattaat tacataaaat ctaaaatagt ttgtaatag
//
LOCUS       DAYAOX010000123        10944 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.123, whole genome shotgun sequence.
ACCESSION   DAYAOX010000123 DAYAOX010000000
VERSION     DAYAOX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.123"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(351..1394)
                     /locus_tag="ACGI57_002467"
     CDS             complement(351..1394)
                     /locus_tag="ACGI57_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717542.1"
                     /translation="MQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEVKQ
                     EMTVMKEDMSEVKQEINVLKEDMSEMKQEMTVMKEDMSEVKQEINVLKGDMSEVKQEM
                     IVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEMTVMKEDMSEVKQEINV
                     LKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMK
                     EDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMKEI
                     QIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATS
                     KNWSDIVKLKSVK"
     gene            complement(1630..2715)
                     /locus_tag="ACGI57_002468"
     CDS             complement(1630..2715)
                     /locus_tag="ACGI57_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFG9717543.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2751..4064)
                     /locus_tag="ACGI57_002469"
     CDS             complement(2751..4064)
                     /locus_tag="ACGI57_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9717544.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(4065..6356)
                     /locus_tag="ACGI57_002470"
     CDS             complement(4065..6356)
                     /locus_tag="ACGI57_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFG9717545.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGKTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6346..7140)
                     /locus_tag="ACGI57_002471"
     CDS             complement(6346..7140)
                     /locus_tag="ACGI57_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFG9717546.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMTKALVVRMYNSIGGECDGK"
     gene            complement(7179..7625)
                     /locus_tag="ACGI57_002472"
     CDS             complement(7179..7625)
                     /locus_tag="ACGI57_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFG9717547.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7697..9103)
                     /locus_tag="ACGI57_002473"
     CDS             complement(7697..9103)
                     /locus_tag="ACGI57_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9717548.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     IRGTIKNRMEGHKWNSLY"
     gene            complement(9121..10491)
                     /gene="hydA"
                     /locus_tag="ACGI57_002474"
     CDS             complement(9121..10491)
                     /gene="hydA"
                     /locus_tag="ACGI57_002474"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HFG9717549.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKIAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10624..>10944)
                     /locus_tag="ACGI57_002475"
     CDS             complement(10624..>10944)
                     /locus_tag="ACGI57_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9717550.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
BASE COUNT         3182 a         2197 c         1138 g         4427 t
ORIGIN      
        1 atattatttt tatagtaaaa attttattca tattactatt tataattttt atagagcaat
       61 tttagtgcca atatttgcat acatttgtat agtataaaat ttttatgttc tcattgataa
      121 aattttactt atttcaagcg ttctattaga ttttatcatc taaattttag gttttatttt
      181 tataaattaa ttgtatttac ataaaaaaaa agacctaaaa acaaattttt aacggtcttt
      241 tctcaatttt ttattttcat ttctaaaatt tataataaat agtaagaaca ctataaattc
      301 ttttatataa aattcttagt ttgtggatga taaattagtg aattaaaata ctactttact
      361 gattttagtt tgacaatatc actccaattt ttactagtgg ctaactctac taaatttaag
      421 tctcttctta tttcagctat atcctctttt atagaattag tgtcaccttt tatatatgct
      481 atgttatgtt ctatattatc atgagtagct ttatttattt cagaattatg taataaactt
      541 cctaaaatat tattctgttc tatctgtatc tctttcattg atttatcttc acattctaga
      601 ctatcaatct taatttcaat tccatataat ttttcatttg ctatggatac ctcgtcctta
      661 attccattca tatcttcatt aatgttattt attttatttt gcataaagtt tacttctgat
      721 ttaacttcgg aaacatcttc tttcattaca gttatttctt gtctcacttc tgacatgtct
      781 tttttcatta catttatctc ttgttttacc tctgatgtat cttctttcat tacggtcatt
      841 tcttgtctta cttcggacat atcttctttc attatattta tctcttgtct tacttcggac
      901 atatcttctt ttaatacatt tatctcctgt tttacttccg acatatcttc tttcattaca
      961 gtcatttctt gtcttacttc cgacatatcc tctttcattg tatttatctc ttgtcttacc
     1021 tcggacatat cctctttcat tatatttatc tcttgtctta cctcggacat atcttctttc
     1081 attacaatca tttcctgttt tacttctgac atatctcctt ttaatacatt tatttcctgt
     1141 tttacttccg acatatcttc tttcattaca gtcatttctt gtttcatttc cgacatatct
     1201 tcttttaata catttatttc ctgttttact tccgacatgt cttctttcat tacagtcatt
     1261 tcttgtttta cttccgacat atcttctttt aacacattta tttcttgttt tacttccgac
     1321 atatcatttt ttatgctaac tacatcttca tttatagtat ataataactc taaaatcttc
     1381 tcttgcactt gcatcaccat ccctattaat tattataaca ttttacacaa atgctataca
     1441 atgttaacat atatatcaga attattaaaa ttttaattaa acaaaattga ttcactttat
     1501 tacttcaaaa ctaattaata tctaaaataa ctacaaataa acaaaaataa ggtcaacaac
     1561 aaaaaaggct tatctctttg agatagcctt tcttattttt atttaaaact ttgtttttta
     1621 taaaataatc tatacattca gctctgaaca tgaaattttt cttccaagtg cttttggttt
     1681 acaaactgat atacaaagtc cacaatatct acactcttct tcattcaatt ccatcttatc
     1741 attcaatttt ctcgcttgat atgcacaaac tctctcgcac atcttacatt tagtacattt
     1801 ttcttcatca taagtaaact caaagctaag tctttcatta acatctccca tatgattatg
     1861 tactatacca caagcatttt ctatagtatt atatcctaat ctattaagat tagattttaa
     1921 atcatcatgt attttagata ttacttgtgc accttttaaa attactactg aacatatacc
     1981 aacaaagtca gcacctgcca taatcatttc taaggcatca tctccactta cacatccacc
     2041 taagccaatt attggtatat taacttgttt ttttatttca tatattctct gtaaagttat
     2101 tggcttgata gtttctccac ttaaatatcc tattccattt ccacctaata aaggttgccc
     2161 tgtttctatg tctattctaa atgctggtcc tacagagtca caagcagtta ttgcatctgc
     2221 tcctgcttct tctaattttt tagcaaaatc gcctatctca tctatcatag gaggaagttt
     2281 aactattaca ggtatattta ctctttcctt tgcatcttta agcattggta tcaaatctct
     2341 atagtcataa gatactaatt caataaagtc agctcctgca tttgcaactt tctctactaa
     2401 ttcgctactt tcttcaattg tatgacctat tgaagctata cagacagtcc catctctttt
     2461 catttgtggt atttcaaaat ctatccattg ctctggctca aaatctgtcc atttttcatt
     2521 atttaaaaga gcattagctg tatttctaac catatgtggt gctggattta tagctctttc
     2581 ttttcttata gtcttagtca caactgcccc tgctcctgca tcacttaata tcttgcatcc
     2641 agctgcacta tatgttaatg gtcctgaacc tattataagt ggtgatttta attctttacc
     2701 taaaaaatta tacattatct gcttcccctt ttcatatata tttacattcg ttaaaatttc
     2761 caaaattcac aagcaacttc tttcatttct ttaacaatag cttttttatc taaatcaact
     2821 aactgttttt cttttaaaat atgtctacca tctatgtata catttgatat aaattctttt
     2881 ttaccctgaa ctactatctg gtcaaatata ttatcaagag tcacaggagt tttaaattca
     2941 tcttccataa ctattatatc agccttatta ccaactgcta ttttaccaga attttgaagt
     3001 cctaatacat aagcaccatg ctctgttgcc attctaaata ctaaatttgc tggcattact
     3061 gatgcatctt cttcaactga tttatgcatt aaaaatgcac ctctcataac ttcaaaaaaa
     3121 tcatttatat atccatcagt acccagtgcc acttttatgc cttttttaat catttttggt
     3181 actggagaaa aacctccacc tacttcacag ttactaagag gcatatgaac cacttttacg
     3241 cctttttctt ttaatatatc tatttcttca tcatttactt ttacacactg agatgctaat
     3301 acagtttcat ccaatatatc taaatcatta tagtagtcaa ctgctttttt gccaaagtgt
     3361 ttttcagcat agtttggctc atatatactc tcacataaat gaaattgcat tggtgcattt
     3421 aatttttttg catcttcttt agctttctta ataaatctat cagaacatgt gaagcttgta
     3481 tgtgtacaca ttattccatt tattaattta ctattttctc tactccactt tattaaatta
     3541 gaattttcat ctaaacatct aagtccattt tcaaagctta ttctttcaca actttctaag
     3601 gataaaatcg ctttcatgcc tatttcttct agtatttttc cttgctcaat taaagttcct
     3661 tcttctgtat ttggagcttc tagggtatca caaaaagcta ccacaccaga ttctataagt
     3721 tctattgctg atgctttagt tgttgccttc acttctttta atccaattct attttctata
     3781 aatggccacc aatagtcatt taaaaaacct tcaaagtcag taaaatgtac atttgccggt
     3841 attcccctag aaagaactcc atattgatgc atatgaccat tgacgaatcc tggcattatt
     3901 atagcatctg atttatctat tatttcttca atatttttat attttatttc aacttcttca
     3961 tttggcaata tatcttttat aatgtcattt tctactacaa cagcagaatt tgtatatatt
     4021 tcatcagcag aagatattaa gtatttactt ttaatagcaa tcatttattt acctccctga
     4081 tttaattttt tcatggccat ataaactttc tcagaagtta taggaagttc tcttatacga
     4141 actcctgtgg cattgtatat tgcatttgct attgctggtg caggtgtatg aataactatt
     4201 tctcctaaag atttagctcc atatggacct gttggctcat aattatctac aaaatcaaca
     4261 attatgtttc ctatatcttt ttttgttgga actttgtatt gtaatagatt agaagtttga
     4321 agctttccat cagaaccatg tctagggtct tcatacattg caagccctat accttgagtt
     4381 acaccacctt caacttgtat tctagcaagt gcaggattca taactgtacc acaatctgta
     4441 gaacaagcat aatttattac tttaaactcc cctgttgttt tatctaactc tatttcagca
     4501 aatcctgcta agaatggttt agcactctct tgtataccat aagattcaga tgaatataaa
     4561 acttcttgat ttccaccacc aacagattct tttcctaatt cacttaacaa aacaaacttc
     4621 tctacatctt cactataaca aactctgtct tcatatagta ttaaatcatc tattggtaaa
     4681 tcgagtttct tctgtgctac ttttaatatc ttttgtttta aactttcagc agattttatt
     4741 gtagcatttc cagttacata agtagtacaa gaagcgtatg caccagtatc atatggacac
     4801 atatctgtat ctcctgtata aacggaaatt ctatcaatag atgtattcaa tgcttttgca
     4861 gctatttgtg caagtattgt gtcagaacca gttcctaagt ctgaagaccc agaaaacaac
     4921 ttataagttc catcttcatc catcctcata tttactattg ccatgtctat gttggctata
     4981 ccagaaccat gagttcccac agacattcca acagccctca ccttattgtt tccaatatcc
     5041 ctaacagggt acttttcatc ccatccaata agttttttac ctctaattat acattcatca
     5101 agtgcacaac ttcttattgg aaaattcatt attccgcctt cagtttgttt ttttataata
     5161 ttctttaact ttatttctgt tgggtccata ttaagtttat gcgctaattc atcaattgca
     5221 caatcaagtg caaatgtacc ttgtgttgct ccatatcctc ttaatgctcc tcctggaact
     5281 aaattagtat atacagtttt cccatcaaat tttattgcag gtacattatt atatgttgga
     5341 agtacattat gacctgattc agagcaaact gaaggtccat tatcgccata agcgcctgta
     5401 ttgttcaatg ctttcatatc tatagctttt atgtttcctt ctttatctgc cccaattttt
     5461 atatcaaaaa acatctgatg tctagtatta gtcattgcaa aagtttcttc tctagtataa
     5521 attagttttg ctggtctttt tgtcatccaa gttacaaatg atgcatatat ctctgttaca
     5581 gctatgtttt ttccaccaaa acctccacct attcttggtt ttattactct cacttttgaa
     5641 agaggtattc ccagtgcttt agaaacctgt cttctcatat gataagctga ttggtttgca
     5701 gaagttacta ctaatctgcc attcgtatct atatacgtat aagctctatg agtttccatc
     5761 atacaatggg cttgtgattg agtcatataa gaatgttcta ctattacatc actttcttta
     5821 aactcttttt ctacatcccc tttacccatt ttttctctcg atacaatatt ttttgtagca
     5881 tccaatccaa aatcaaatgg acaaaacaaa tcatcttctt catggattaa aattttattt
     5941 ccttccgatt ctcttgcatc caatatagga gtcattactt catattcaac ttttataagt
     6001 ttcatagctt taatagcagc cctttcatct tcagcggcta ttatagcaac ttcatctcct
     6061 acatatctaa ctacgtcttc aagtattaat tggtcatgag gtgatgcttc tggatatgac
     6121 tctccaacca ttgaatatct ataatttgga acatctttgt aagtgaatat gtttactaca
     6181 ccttttacct ttaatgcatt ttttatatct atgtttttta tcttcgcaaa agcatgtgga
     6241 cttctaagaa gcttaattgt taaaacatct ttatgaaaaa ttaaatcttc tgtatatatt
     6301 ggttttccag taacaatggc ttcactatct atttttctta cctctttatt taccatcaca
     6361 ctcacctcca atactattat acattctcac tactaaggct tttgtcatat cttttctgta
     6421 ttctttactt cctcttatat tacttccaaa tgaaagttct tcacttgata cttcaccagc
     6481 tttttctata tcattttcta aacttaatat ttcacttgct ttataggcaa tttttgctct
     6541 tcttggtcta gcgcctatcg caattttata aatacttcct tcttttaaca ttgctccatt
     6601 taatactgca aaatcacctg tacattttct tatagaatca aatacaccta tagcatcttt
     6661 tttaggtaat ataacttcta ttaatatatc tttttcaagc tcattttcta aaaaatcagc
     6721 taagttcata accccttttt tatataactt aacctttgca tctaccacta gtaatgaagg
     6781 tattaaatct gaaaatccat acttagcaaa tacgctagct cctactctag ctccacttct
     6841 aaattgaact cctactatat tagaaacagc gtttgaaatt accccattac agtaattttt
     6901 aataacctta tttaattcta aagttcttaa acttgtatct gctccaatta atatgctatc
     6961 atcagtttca tttatataat ctaaagctaa gtcttttaaa tctattgctg aaccaatatt
     7021 tttactacct agttttaaaa atccacatcc tccaagtatt atgttgtttt ttcttgatat
     7081 tagcagttca taagcttcat ctaaactttt tggaactaca tattccttta tagttatcat
     7141 actctctccc catatcaact taattttcac ttaaaagtct aagtgtttaa atatttatgt
     7201 attgctctta attgaccttt atatcctgta catctacaaa ggttaccatt tacataatct
     7261 ataacttctt cttctgttgg attttgaagc tctcttttca ttgccaagac catcattata
     7321 aatccaggag cacaaaatcc acactggtca gccccttcgt ctgcaagaaa atctccaaac
     7381 ttctttgctt catccaagac accctcaagt gttgtaatat gctgaccttc tactctagca
     7441 gtaagataat tacatgaaag cacggccttg tcatttacca tcactgtaca taacccacat
     7501 gagcctgtat cacacccttt tttaacactt acatatccat tttctcttaa tgtatctgct
     7561 aaaaaatcat ctggtcttat acttaattct ttttctttat tatttatggt acatttgata
     7621 atcaaataaa tcacctcaaa actcatattt ttaagctaaa gttttactat acttttttat
     7681 gaataaatta catttattaa taaaggctat tccatttatg accttccatt ctatttttta
     7741 tagttcctct tatacaaagt ctctcagctg tatcttgagc atcttttaat gctttctctt
     7801 cattagctgt caatattttt gaatcttcca ttattatatt tccatctact ataacactct
     7861 ctatattttt catactagaa gaatatacta atgtagatac cggattatgc ataggtatcg
     7921 cttttggtga aagcattgga ttaaatatta gtaagtctgc ttttttacct atctccaatg
     7981 aacctatctc atcttccatt ccaatagctc ttgctccatc tatagttgcc aactcaagaa
     8041 ctttttctgc cgacattgct aatgggtcac acttattaac tttatgttga agtgctgtta
     8101 atttcataag ttcaagcata tcttgagagt tattactagc ggctccatct actcctaaac
     8161 taactgttat tcctttttta agcatttctg gcactggtgc aacacctgaa gataagtaca
     8221 tattacttgc tgtattatga gacactttca tatcgtattt tttagtaagt tccatatctt
     8281 tttcagttag atacacacaa tgtaccataa gcacatttgg tccaagtatt cctaattttt
     8341 ctaaaacatc tatgtcatat tgcccatgca attcttttgc tgcttctcta tcaaaaggag
     8401 tctctgatat atgtactgta aataaagcat catcatattc ttttactact ctccatagca
     8461 tttcaagcat ttcttgagaa tttgaccata ttgctgcagg agctactcct attttgattc
     8521 ttccattttc tgtattatgg tgtttttcaa acaatcttct tacatctttt tcaaccgttt
     8581 ctacatcttg cattattcca ggatgaactc caaattgagc tcctgtattc atacatcctc
     8641 ttccaagtat tcctcttatt cctaattctt tataagcatc tattattcca tcacaaagac
     8701 ctggcttact atgaggatac atgtagtcaa ccattgttgt tataccactt ctaagtcctt
     8761 caatacatcc aagcattgca gcatcataag tatctttggg ctctaaatag ttagctgctg
     8821 gaaatgtcat agtttctaac cagtctttaa gtaccatatc atctcccaat ccttttaata
     8881 aagtttggaa taaatgatta tgtgtattta taaaacctgg gaataaaact ttacctttag
     8941 catctataat ttttttaaca tcagtatatt tactttctat ctctttagaa tttccaatat
     9001 cagcaatctt gttatctttt actactaatg ccccatcaaa aataacttct ctctctttat
     9061 tcacagttac tattatggca ttcttaataa gtatatccat cattcttatt tccccccgca
     9121 ttatttagta atttttcttt taatatattg acctgttgga tgttctttaa cattacaact
     9181 ttgtactaca tgatttcctc tcaaaaatac atcttcaact ttacatttaa ccttaaatcc
     9241 ttcatatgga gtataatcta cattttgttt ttgagtttta taactaattg tttcttcttt
     9301 attgatattt aaaatgacta tatctgcatc acttcctaca gctatctcac cttttttagg
     9361 atacattcca aatatttttg cagcattcgt agaagtaact tccacaaact tatttacact
     9421 tattctattt tctaagaccc catatgtata taaaagtgct aacctatgtt caacaccagg
     9481 tgctccattt ggtatcttac tgaaatcatc aattcccaag tctttttgcc ccttaaaatt
     9541 aaatgaacag tggtctgtgc caactgtttg aatatctcca ctagacaatc cttcccaaag
     9601 gtaatttatg tcttcttttt ttcttaaagg aggagacatt acatacttag caccctcaaa
     9661 tttatcctta ttatataaat tgtcttctaa taatagatac tgtgggcatg tttctactat
     9721 catatctata tttttttctc tagctatttt tacagtttca agtgactccc tacaacttaa
     9781 gtggactaca taggattttg aattagataa tttagttata tcagcaagtc ttgaaacaga
     9841 ctctttctca actatgttag gtcttgtaag tggatgatat tttggctcca aattaccatt
     9901 ttgtatattt tcttctatca atacatctag taaatcccca ttttcacaat gaaatccaac
     9961 tatacaacct aaatcccttg ctttctttaa aaccctgtat attgttccat catctacttt
    10021 catgccatca tatgccatat acatcttaaa agatactatc ccatctgaaa taagtttttc
    10081 catatgatta aatgtatctt tatttaaaca tgtaattgtc atatgaaaac cataatcaca
    10141 ataacaattt ccacttgctt ttttatgata tgcttctaca ccttgcagta gattttcccc
    10201 ctcattggct tctgcaaaat ctagtattgt agtagttcct ccagctatag ctgctcttgt
    10261 tcctgtttca aaattatctg cagttgtaat agaacctgca ttcatatcaa aatgagtatg
    10321 tgtgtctatt ccaccaggaa ttacaatttt acctgtagca tcaataacct tatcaccatt
    10381 tatttctaag ttatttccta tttcaactat cttttcatct tctatcctta aatcacttat
    10441 ataagtctta tcagaggtaa ttatagttcc acctcttagt attgttccca tatatacctc
    10501 ctaactttta agctttaaag tattttctaa gtattgtaaa actttattta aaattgtatt
    10561 ttctaaaggt gctatattca gcacctctag actatattta aataatttat tgtgaactaa
    10621 atattatttt ccgtatacag attttaatat tccatagtaa ccttctgttc ctataaataa
    10681 ctgttcttgc tctatatact catctattgt gtgagcaaga ttttccttag aaggaccaaa
    10741 tcctatagtt tttattccag cttcacctgc atagtgactt ccattcgtac agaatgagta
    10801 ttgagttatt tctgaatcta ttccagcttc ttttaatccc ttatacgctg cttgaacaaa
    10861 ttcatcttct tcgtcatata accaacctgg gaagaatctt tcactttcta tagtatttcc
    10921 tgtatagcaa tcagcttttt caac
//
LOCUS       DAYAOX010000124        10911 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.124, whole genome shotgun sequence.
ACCESSION   DAYAOX010000124 DAYAOX010000000
VERSION     DAYAOX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.124"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..607)
                     /locus_tag="ACGI57_002476"
     CDS             complement(<1..607)
                     /locus_tag="ACGI57_002476"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFG9717551.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQ"
     gene            complement(700..1032)
                     /locus_tag="ACGI57_002477"
                     /pseudo
     CDS             complement(700..1032)
                     /locus_tag="ACGI57_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            complement(1110..1313)
                     /locus_tag="ACGI57_002478"
     CDS             complement(1110..1313)
                     /locus_tag="ACGI57_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFG9717552.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(1703..1933)
                     /locus_tag="ACGI57_002479"
     CDS             complement(1703..1933)
                     /locus_tag="ACGI57_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9717553.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1935..2288)
                     /locus_tag="ACGI57_002480"
     CDS             complement(1935..2288)
                     /locus_tag="ACGI57_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFG9717554.1"
                     /translation="MRNYARDIYDENKRRNDYLVSLESLSLAELSKLSILDDYDSNYI
                     CMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMTNADIATLMDLAENTIHYH
                     KTNALKELKELMQEH"
     gene            complement(2702..3151)
                     /locus_tag="ACGI57_002481"
                     /pseudo
     CDS             complement(2702..3151)
                     /locus_tag="ACGI57_002481"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3165..4091)
                     /locus_tag="ACGI57_002482"
     CDS             complement(3165..4091)
                     /locus_tag="ACGI57_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717555.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            complement(4216..4998)
                     /locus_tag="ACGI57_002483"
     CDS             complement(4216..4998)
                     /locus_tag="ACGI57_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFG9717556.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5154..5774)
                     /locus_tag="ACGI57_002484"
     CDS             complement(5154..5774)
                     /locus_tag="ACGI57_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9717557.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5901..6569)
                     /locus_tag="ACGI57_002485"
                     /pseudo
     CDS             complement(5901..6569)
                     /locus_tag="ACGI57_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6640..7476
                     /locus_tag="ACGI57_002486"
                     /pseudo
     CDS             6640..7476
                     /locus_tag="ACGI57_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            8148..8915
                     /gene="aroD"
                     /locus_tag="ACGI57_002487"
     CDS             8148..8915
                     /gene="aroD"
                     /locus_tag="ACGI57_002487"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFG9717558.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9243..9920)
                     /locus_tag="ACGI57_002488"
     CDS             complement(9243..9920)
                     /locus_tag="ACGI57_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717559.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10070..10387)
                     /locus_tag="ACGI57_002489"
     CDS             complement(10070..10387)
                     /locus_tag="ACGI57_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9717560.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10409..10747)
                     /locus_tag="ACGI57_002490"
     CDS             complement(10409..10747)
                     /locus_tag="ACGI57_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9717561.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3608 a         1731 c         1560 g         4012 t
ORIGIN      
        1 attgaatatg cttaatgttt gattgaattt ttttatatgg ccaatcccac catttaattt
       61 ctaataattt tgaaattgta tcattattaa atctcttttt tataattttt gcaggtactc
      121 ctccaacaat agaatacggt ggaacgtcat tagtaactac tgctcttgtt ccaataattg
      181 caccatcacc aatatttaca cctgacataa ttatagaatc ataacctatc cacacatcat
      241 ttccaattat aatgttaccc ttattgtccc aagcgtccgt tatatggttg atatcgagtt
      301 tccattcttc ataaaagatg ggaaaagtat atgtggattt tgatttcatt gagtgatttc
      361 cactagtcat tagaaatttt gcattacatg ctattgagca aaacttacca attattaatt
      421 tatcattgtt tatagggtaa tgatacaata cattgttttt ctcaaaatct cttgggtcat
      481 tataaaagtc attatatatt gtgtaatctc caacttctat attttccctt gttattacat
      541 ttttaagata tacagtttga taatcattac ttcttggata taccttattt gaacttggaa
      601 tagtcatatt taaatctctc ctttaatata tattatgttt ttgactattc cactactaca
      661 atacatctct tcacagaatc actccttgtt aagaatcttc ataagataat ggcgtccttt
      721 tttagtgtta agtcggatat tctttcttag tccattcttt ttagcgtata actgacaaac
      781 tgtcattttc ttaccaatag tgtcaatgct atcatcaagg tcattttgta ttgctttttc
      841 cttatccctt aatgatatat catcacgttt tccctttggg ggtttatcag taggtacaag
      901 tttccaagag taaacaaatt gtggtttacc aaatgaatat acatacttat atgcgtatct
      961 tccatctttt ctctggctct ctccagttct taaaattcta tctttattat ctcgtctttt
     1021 ttcagttgac atgataagaa gctcctctca ataaccgaat tgagcttgat acgtcatatg
     1081 aatattataa catataaggc tcaatttcat tagataacgt caagtgtatc aacgattttc
     1141 tcaaattgtt ttctctttat ttgaatgcga ttgctattca taatgaccca gccagaatca
     1201 aggttttcat cagcacgttt acgtaatttt ttctctccaa tacgaaagta ttttgacgct
     1261 tcttcaatcg tcaaggtata tttttcccaa ataggtacat cagtattgtt catataatgt
     1321 atcactcctt tctatgttcg tagttcagaa gtagatagat ggctttgata agctccattg
     1381 gtatttcaac caatcccatt cttatggaca gaaccgtgtc atacaggtgt atcattatac
     1441 tgtggtacag gttacggcaa aacaaccgtt ccaaaggtta ttcacaaatc actatatagt
     1501 agctctcttg attaaggtca tggcgtatag tatttgttgg ctatctgtat cacaaacgta
     1561 tctatctact ttattcagtt gtcaaagaac agtcggcatt gatttgccta taaaaaatca
     1621 caatagcttg tgggctttta tagacaaata attagtgatt ctccatgtac caaatagctt
     1681 gatacatgga gagagggcga gattaagaca aattaaatct caaaatagca gtgattagtt
     1741 ttgttcctag cattcgcttt gtttcttctt ctacatgaat atagggattt ccgtattcat
     1801 caaataaagt tttctgtgaa agacgaatga tatagtgttc aaaatgttta agtattacgt
     1861 ttattgcatt tacatcgcca tgtacagcaa gtaaaataat tgtaaaaggt aatacttctt
     1921 tagtaggttt catttcaatg ttcctgcatg agttctttca actcttttag tgcatttgtt
     1981 ttgtgataat gtatggtatt ttctgctaaa tccatcaatg ttgctatgtc agcatttgtc
     2041 atgttgaaaa agaatgaaag tagaattata tcttgttttc tttttgtaag ttttccgatt
     2101 gcttgcgcca ttaatacatc ttcaattcgt atgttgtagt cgtaagaaac catacagatg
     2161 tagttagaat cataatcatc taaaatactc aatttactta attctgcaag tgacaaagat
     2221 tcgagagata ccaaataatc atttctgcgt ttattttcgt cataaatatc tctagcatag
     2281 ttacgcaaaa ctgttttaca gaaactgtcg aattgatgtt caattgtctt ttcatcaacc
     2341 ataagatttc accctctttc catcgcaagg agttaaataa ccttgcttac tcctatcacg
     2401 gtgggagggc gttaaatctt cgataaattt caaaaaaaag agaaaaaaat acacactctg
     2461 cctatagggc aaaaatgtgt attctttaaa acgtatttaa tttatattgg ttgtaagcca
     2521 ccaccatatt gatgtatgga aatatgcatt ggctagcttt tcataaaaat acaaagatgc
     2581 ataagaaatt aatagagggg aaaaacttat tctgttatta aaaagtaggt taattctaat
     2641 aaatgctatt gcggtcgaat taaactatat acatggcaat caatcatgtt tctattcttt
     2701 attatgaatt gtgacagtat tcttcaatct taaagccaca tttttccaat actttacttg
     2761 actctctatt gtaggaatat acattagcat gaattttgtt aaggttgtat ttttcaaagg
     2821 caattttgca tatttctttt actgcatttg ttgctatccc tttgcctcaa tagttcctag
     2881 caagccagta acctaattca gcatttttat taatagaggt atcttcttca aatgttaggc
     2941 tgatacttcc aatagccctt tcttgataaa aaatagcata gctaagttgt ttaacatctg
     3001 aagttgccaa acaaaaacta acaaaatctc tagcgttttg ctccctataa gggtggggga
     3061 agttgtcacg aagattcttt gcaatttctt tatcattcgc aaactcaact aaatcggtaa
     3121 tataagcata attccactct ataagttcca tttttcttct ccttttatga aacagctaca
     3181 agaaatcttt ctaagccaat accacttatc aaacagcgtt ctcttttatt tccaagcatt
     3241 tgataagtcc agtcaacaaa tccaccgtca actatttcaa cggtttcatt tccttgtttg
     3301 accgataatt tgaaattgat acctttataa taattattat caacatcatc attatgaatt
     3361 gataactcta tattaggaaa aataagtttg atagtttcaa ccattctgtc gaaaaatccg
     3421 tcattatctt tgtatccgtt tcttttgcgt agaataatag agaaacgaac attttctatt
     3481 tcacttaata tatctttata atagtttaag tgcttttcta acaacatttt ttcacaagtg
     3541 taagaccctg tatctatacc agatgaaacc atacaatata agccaaaatg tgaatataaa
     3601 cctttaccta caaaattttg tgctcttatt accctagcag tagtagccat gtggataggt
     3661 atggtattat tttcttcttt tctttttaat ttatcagcaa taattattgc caacatattt
     3721 gaggggtctg caagtgtttc tgtccctcgt agagcactta ctacgttata ttgatctacg
     3781 gagccaaaaa cgctacagct acctaatggg gcagagggag aaagcataat cgtcttgata
     3841 tccatattga atgcttttct caataatttt gattccaaag catgaatttc ttctggatct
     3901 ataccgctag ggatagtaaa tctatttagt tggtatgact ttagaatatc agttgcagtt
     3961 aaagtatttg cttgtctatc aaacacttct aatagaagtg agttcaaatc tgattttgat
     4021 agagctgata acttatcaac tatattctta tccccaattt tatttgtaat tctacttaaa
     4081 atatcactca tagttatcct ccatactgtt actttcaaaa taattataga attgttacaa
     4141 caactaatct aggtcatttg accaaaaatg aaaaaacact gaatatttac tattcaatat
     4201 ttttttactt tgggtttatt tgattaaatc ttgaaaatac tgtaatgcaa ccctttcagc
     4261 ttcaaccttt cggacaatag gactttgtga taattcctct cttaactcaa gatacaggtt
     4321 gatagtagcc tgcaatgcat taacacagct tttatattca tggttagcaa ccgtcttttc
     4381 taatgcttca atatattggg gtgcgtccaa ttcaattttt ctaactccac gaggtaattt
     4441 acctaagaga agctgtatta atggggcaag tactgattgt cttaaaaaag ataaaaaatc
     4501 aattgtttca aatagttctc tacgtccaat cttagtgcaa gcgtaatgaa cccaaatcca
     4561 aaatctatct tcaatccact gtaagtctgg agttgggaag tatgcttctt ttttagaata
     4621 aatttctgtt atgcagttgt tttcttcata gagaatagct gaatcttcaa ccctatctag
     4681 taaaccatct aaggttgtaa acttaaaatc aacatgaaga acaggggaat catagaggga
     4741 aattatcaat cgaggttctc caacgtgttc gccagtaaaa gcagaaacta atgttccaaa
     4801 ctgttccaca ataagtaatc tctcttcgat aacttcttca taggatttat catctgctac
     4861 aatcagaaaa tccaaatcag aatattcatc taacgatttt gtgatataag aactgctaat
     4921 tgcaagtcct attatcctac tatctctttt tgaaaattct aatacgtttt gtatcatttt
     4981 ctgttgtgtt ttagtcataa tataacacct ctcactgtct tttattagct atcaatccaa
     5041 cataattatg cataggagta ttatataatg aagaaataaa aaaaaatagg tcacttgacc
     5101 cgaaataaaa aagcattgaa taattattat tcaatacttt tttgaaatta tacttatgtt
     5161 gtaggaagat tctttaatac ctctcgctga ttaattacac taaatctttt gttttcataa
     5221 gcaattagat gttcttgtac acattcattt atagcacgat taatgcttct aattggagcg
     5281 tttacttcgc tagcaagctg ttttttggta agcagattta attcacttct ataatagtgt
     5341 aaggatatac acctcagaag tcgttcttta acacttaaag atgataattc ttcaattaat
     5401 tcagcattta caactaattt actagatatt tctcgaatca gaaattttgt agcttcaaaa
     5461 tctcccctta accagtttaa aaaatcatct ctatgaagtt tatatacatt acaatctgtg
     5521 aaagcgacaa tttcaacggg ttttttccca tcattaaatt gttccatttc tccaaaaaaa
     5581 ctaccacttc tataaagatg aatcgttgag atgtttcctt tcacatttaa gatgaatgct
     5641 tctgcaatac cgtcaatcat aagaattaca tattcatttt cttgctcagc aaaaagaata
     5701 gtctctttct ttttatacgt ttttttaaca agcttctttt taatatcttc attcataaaa
     5761 tcaattaaat gcatattcat ccctccttac caaatctttg attaaattat atcatctttc
     5821 aattagttag gtaagatagt aaaaatatat ttacatagaa actattccat tattattttc
     5881 cagttgtcat ttttctgcaa aaacttatca ctgtcacgac tggagatacc agtaaggaat
     5941 gttaggatat caatcgctaa actttcagaa tcctttttac ggctcaaaat gacgtaaggg
     6001 tcaagcaatc ctttgttttc atcattactg gttaaattga caatgtttat ctcatgggct
     6061 atttctggta gcgtttcttt ccatttgccg cgttctgctt tagggtcaac tatgaccgcc
     6121 tgtccgccga atagtaccgc atagtaaaca agcatattgt tactgaataa tttaccacca
     6181 cccaatgaac cgataaacgc agacgctaac gcattggtaa tagaaccctt taccccttga
     6241 ctagcaaggg caggttttag atagacattc tttccagtgt ccagattgta gccaagatag
     6301 atatcctcat gttcgccaag ggtttgtgtc gctccaaaac caagtccagc caaaaaatcc
     6361 gaagtgacat attgaatata atcattgatg taacgtttgc tgacaggaat aaattcgcca
     6421 tgcaatccca acatatcccc aaagggacgc acgagcttga ttgataagtc gtcgtaaaag
     6481 tctttgactt catcacaacg cctttttagc tcgtcaagat tctttgtaga tacacggaca
     6541 acatagctaa gtttatacat agattcttta cagttaaaag tctgttttta aaaaaatgta
     6601 taaattataa atcaaaatat agatactatc aatgacccac taggtgcaaa aagcattgat
     6661 atggttaaat tatcagacgc taaaagctgg gctatccgaa tgagtgaaga tgggtgtgct
     6721 tataaatcca ttgacaatta taagcgctca ctgaaagcgt cgtttttcat ggcaattcaa
     6781 gacgactgta ttagaaagaa tccatttaat ttttagttaa gtgatgtttt ggaagataca
     6841 acagaaccaa aaatcgctct aacaccagaa caggaaatgg gcttattatc ctttatgggg
     6901 agcgataaga cttattgtaa atattatgat gatgttgtta tcctcttaga aacaggacta
     6961 cgcatttctg aattatgcgg tttgacttta gaattggata tgaaaaataa agccattatg
     7021 gtagaccatc aattgttgag tgatactaag attggctatt atgtagaagt tccaaagacc
     7081 acacagggta agcgagaaat tccaatgact gaaagagctt atcaatcatt tgaacgtatc
     7141 gtggaacggc gtaagaacgc taaagcagaa ccaatcattg ttgatggtta ccagaacttc
     7201 ctatttctta aacaagatgg attaccaaaa caagcaactc attatcaaag catgttaaga
     7261 gcgttagtca agaagtataa taagacccat gaggacaagc taccaaagat tacgccacat
     7321 acctttaggc atacactctg tacaaatatg gctaacaaag gaatgagtcc taatatattg
     7381 caatatatta tggggcataa aaacattact atgacgcttg gctattatgc acatggttca
     7441 tttagttctt cgagagctga aatggaacgg ttataatcct agtagtatga ctagagttta
     7501 ctacttattt actacttttg atggcattct tatacggtta aatgccaact tatgcaaagt
     7561 atcttccaaa aagaaaatgc cgatactcct tgtagataca ggatatatcg acatttacca
     7621 acttatgaaa agataatcaa atttaatata agtttttcaa ataaaaagat accaaattct
     7681 ttttcgagaa tctatataac attacttaag cttggttggg gtataaacaa ttgagtagct
     7741 acttttgtat ggtgttcaac tttagtcaaa actcttaagt aatgcaaatg atttagattc
     7801 attaatattc aatctcttca taagtagtga aattcgagtt atttctgcat aattatacta
     7861 tgatttatag attaattcta ttagcatcca gaataatatt gtttatatga gtaaaattta
     7921 aagaatattg tgaaaattat aggatgttaa aataaattta ggtaaaaaag aagtgtttgt
     7981 atattataga gtaccttttc taaaatatgt gggattgttt taagaattag aataaaaatt
     8041 atttttaaat tagtattttt ataatgtata gtatttttaa tgtataatta aattgtgcag
     8101 aaaatagaat ttaaagtcct atagaatttc tgattatgga ggagaatatg aaaagaaaag
     8161 ttcaagtaaa aaatattact attggagaag gtagacctaa aatttgtgta ccaattattg
     8221 ggaaaaataa aaaagatatt ataaaagagg ctaaagagtt aaaagatgca tgtttggata
     8281 ttatagagtg gcgagttgat ttttttgaga atgtagaaaa tattaaagaa gttaaggaag
     8341 ttctctatga attaagaagt tatatacatg atatcccttt attattcaca tttagaagtg
     8401 tagtagaagg gggagaaaaa ttaatatcaa gagattatta tactactttg aataaagaaa
     8461 tttctaatac aggtttagta gatttaatag atgtagagtt atttatggga gatgaggtca
     8521 tagatgaagt agttaatttt gctcataaaa aagaagtgaa agtaattata tctaatcatg
     8581 attttaataa aactccaaaa aaagaagaga ttgtatctcg tttatgtaga atgcaggaat
     8641 tgggcgcaga tttacccaaa atagctgtta tgccgcaaaa tgaaaaagat gtgttagtgc
     8701 tacttgaagc tacaaatgaa atgtttaaaa tttatgcaga tagaccaata ataacaatgt
     8761 caatgtcagg aatgggtgtt ataagtagat tgtgtggtga aatatttggt tctgcattga
     8821 cttttggagc tgcaaaaagt gtttcagcac cagggcaaat atcatttaaa gagttaaatt
     8881 cagtacttaa tctattacat aagagtatta attaaattaa ttatatagaa atattagacg
     8941 gtatttttga agatataaat taaatataaa aaacttttca gatagtaaaa tttaaattgg
     9001 gcaattatat ctgaaaagca actatttagt gtgtattttg aaagtgatat agaaaatctt
     9061 ataaatcaac tatggcatat ttaaaatatg ccattaaaac ctactagaca ttagattata
     9121 gatggtatac tagcatttct attatttata gatagtaagt tttcataata actgttttat
     9181 ctaaagtaga agatatattt gtattatgta tatttgtcat tatttttttg taattatgta
     9241 tattaatggt tatatatgta atataatatt atcactctat tttttgtata ttgtacatta
     9301 caacatttat atctatcatc atcaattaca gtagaatgta tgggatactt attttcactt
     9361 aaccaatatt tttgcatatt ctttgtccaa gactttggaa acttcaatac tttcttgttt
     9421 attataacat cctcatcttc aaaaatatta acttctcttt ctatttttat ttttctgttg
     9481 tatatcttat tgatagattc tttgtcgagt tttatgtctc tatacatatc agggcaatag
     9541 caacaaacat aatttctttt ttttcgacgt ttaaatttca caactttctc tatgctaatt
     9601 tctaattttt ttgtaccatc ttttataaat ttaatatctt ctatgttctc gcttgaaaaa
     9661 atttctgtca atcttaaagt atctacagtt tctctaatta tttgcttttt ataagaagag
     9721 aatccatatc caaaagaaag gtttaattta ttagggatgt agccattttt atatttaaac
     9781 atcttaattt tttccaattc agcattatct ttaaatttca acttttcata cctaaaagtt
     9841 actttgctaa aaagagtata ttctacatca atatattctt ttgcaatctt aaatttatcg
     9901 ctaccttcaa tcacaatcat agcctatctc caatcgtttt atattaaaat aaggccatag
     9961 aacatggtct aatagtactc attagcacta taagacaatg ttaacacaaa aagacaaaaa
    10021 aaggttattt atttcaaata accttttttt ttatataaaa tataaagaat tatattttat
    10081 tcttttttat gatgtctata atctgtgata tcagatttag ttctcttgaa tttaagtctt
    10141 ttaactcttc atttatatca ttttctaaat atgtttttcc agtggaagta atatctttta
    10201 taagaacatc tgcagaaata tttaatatat ttacaaattt tatgaatgtt ttaagacttg
    10261 gattcttttt accactttcg atttgagtta tataccaagg cgatactcca accatatttg
    10321 caaattcttt tgaagttaaa tttgcttttt tccgttcttc tttaagtctt ttacctatat
    10381 aattcattat tgttactatt tccctcactt atatttcttt gtatttaatg atgcacttta
    10441 ttaactctag cattgcaatt tgttcttttt cagttaattt attaatcatt tcataacatt
    10501 ctaaaggtac agagttatca acgccttctg ttgtaataat atttgcaaat aacaattcaa
    10561 gtggtatgtc taaagcagta gcaattttca ttataatatt gatagatgta tttgtctcag
    10621 ctctttcaat ctgaccaata tatgttggat gtaaacctgc cttttctgct agaaactctt
    10681 gactatatcc caattttgtt ctatagactt ttattctatc tcctattatt tttcctatat
    10741 ttgccaatta ttatccctcc actttagatt atattcatag tttaagttgt tttatataga
    10801 ctatttatac ttttttagtc taaatataga ctatatatgt ttttatactg aaataagtgc
    10861 tatttatttt tgtatagtct gtatctacaa taaacaaata tggataaatc a
//
LOCUS       DAYAOX010000125        10843 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.125, whole genome shotgun sequence.
ACCESSION   DAYAOX010000125 DAYAOX010000000
VERSION     DAYAOX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.125"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            286..750
                     /locus_tag="ACGI57_002491"
     CDS             286..750
                     /locus_tag="ACGI57_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFG9717562.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            757..1251
                     /locus_tag="ACGI57_002492"
     CDS             757..1251
                     /locus_tag="ACGI57_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HFG9717563.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1267..2292
                     /locus_tag="ACGI57_002493"
     CDS             1267..2292
                     /locus_tag="ACGI57_002493"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HFG9717564.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2279..4726
                     /locus_tag="ACGI57_002494"
     CDS             2279..4726
                     /locus_tag="ACGI57_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFG9717565.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNKVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4906..6279
                     /gene="radA"
                     /locus_tag="ACGI57_002495"
     CDS             4906..6279
                     /gene="radA"
                     /locus_tag="ACGI57_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFG9717566.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSEKLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6282..7352
                     /gene="disA"
                     /locus_tag="ACGI57_002496"
     CDS             6282..7352
                     /gene="disA"
                     /locus_tag="ACGI57_002496"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HFG9717567.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7441..8535
                     /locus_tag="ACGI57_002497"
     CDS             7441..8535
                     /locus_tag="ACGI57_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFG9717568.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8664..10841
                     /locus_tag="ACGI57_002498"
     CDS             8664..10841
                     /locus_tag="ACGI57_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717569.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         4528 a         1094 c         2011 g         3210 t
ORIGIN      
        1 tttttttatt attattttta caaatgttta gtaataaaga tgagaaggta tatttaaaat
       61 ataaagtatt taaagttctg aaatctaatc atgaagcaaa aaattagcat ttggttatca
      121 ataaatataa taaataaaaa ctcataatta atgaaagaat aaattcatat ctaaatttct
      181 ataaaaaatt taaatttata caatttaggg ctttaaaaat ttaaataata ggtgtaaaat
      241 aatagtcaaa gaaagtcaaa aataaaaagg tgtgtggtga tattaatggc aactatgaca
      301 gatataattg aaaaatttat taaagatttg atggaagaag ataatagcat acaaattcaa
      361 agaaatgaac tagcaaactt gtttagttgt gcaccatcac aaataaatta tgtcttaaca
      421 acaagattta caatagatag aggatattac attgaaagta aaaaaggtgg cggaggatat
      481 gttcaaatag aaaaaatacg aaaaagtaaa gatgggcata ttagagaact actaaatgaa
      541 aaaataggaa gtcagatatc ctataaaaaa gcaaaagaat tattagaagg tttaaaagag
      601 tcggatttga ttaacgaaag agaattaaaa ttaatattat atgcaataga tgataaatca
      661 ttgtgtatgc caatatatga attaaaagag aaggtaaggt ctaatatttt aaagaatata
      721 ataataggat tatttagtat agaggagtga taatttatgc tatgtcaaaa atgcaataag
      781 aataaagcat ctgtatatta taataagata gtcaatggag aaaaaacaga gatgtattta
      841 tgtagtgagt gtgcaaaaga gaatacagaa atgaatttta acttagatat gcctttttca
      901 atgatggata tattttctaa tttaggtttt caacctaaaa aagaacttga ggaaaagcta
      961 gtttgtccta aatgcaatac aacatatagt gagtttaaga ataatgggag atttggatgt
     1021 agtgaatgtt ataatgcatt tagtagtcaa gtaaatccaa tgcttcaaaa tatacatggg
     1081 catatagaac atacaggaaa agcacctaaa aaatcttttt ataagataag tgtagaaaat
     1141 gagattaaag aactaaaaga agatttagat agagctataa aaaatgaaga gtatgagtta
     1201 gcagctcaat ttagagataa aattaaatat ttaaaaggtt ctattgacta agattggagt
     1261 gatattatga aagagaacat agtcatgaaa tcaagagtta ggctagctag aaatttaaat
     1321 aactatccat ttccaaataa attagataaa gaatgtgcca tggaaataat agaaaaagta
     1381 aaaaatgcgt ttattaattc taacttagaa caaaaagaag aatttgattt ctataaaatt
     1441 gaagatttag accaatctaa aaaaatgctt atggttgaag aacatataat aagcccagat
     1501 ttagcagaaa atgataaaag tgcagttata gtaaaaaaag ataaaactat aagtataatg
     1561 ataaatgaag aagaccatat aaggatacaa actatatgtg atgatttaaa cttagaatat
     1621 gcatatagtg tagcaaatga aatagatgat ttgttagaat caagtttaga atatgccttt
     1681 aacacaaagc taggttatct aacatcttgt ccaactaata caggaacagg aatgagagct
     1741 tctgtaatga tgcatttacc agcattaagt caactaggct atatggatga actttataaa
     1801 atttcatcac aaataggtat agcaattaga gggatatatg gagaacgaac agaagcgttg
     1861 ggaaatatat atcaaatatc taatcaactt acacttggaa gaacagagtc taatattata
     1921 gaaaatgtaa gtggacttac aaaagatgca atttcaaaag aaataaaagc tagagaaata
     1981 ttgcaaaaaa aattaggtat aaaactagaa gataaaatat ttagatcgat agggacactt
     2041 gaaaattcaa gagtaatgag tagcgcagag gcaatgagcc atctatcaaa tataaaaatg
     2101 ggaatagaaa tgaactatat agataaacta gatttaaggg ctatagaaca acttatgata
     2161 ggtatacaac cagcgcatca gtcaataatg tataagagtg atgatgttga gaatagagat
     2221 ataaatagag caacatatat tagagaaact ttggaaaaat taagaggagg gatgaattgt
     2281 gaacttcaat agatttacac aaagagctaa aaaggcaatt gatttagcgt ttgaatctgc
     2341 taaaagtcta ggacacaata ttgtcggaag tgaacatata cttttaggtc ttttaagaga
     2401 agaagaaggt atagctgcta aagttctaag taaagttgga tttacagaag cttatttaga
     2461 aggcaaaata gttgatatgg aaggtaaggg agaagaaatc tcagaagata tagtattaag
     2521 tccaaggagt aaacaaatac ttgaattatc aggaatgttt gcaaataaat taaaaacaaa
     2581 ttatattggg actgagcata tattattagc aattattcaa gaaggtgaag gaatagctaa
     2641 taagatttta aattatgctg gagtaaatga taggacttta gcccaattaa caattgatat
     2701 gatgggtatt agtgataaaa atcaatacaa agcagagaat agttacacgg gcaaccagaa
     2761 tcaagcagaa tctaaagtac ttgataagta tggaagaaat cttacactat atgcaaaaca
     2821 gaataaaata gaccctgtaa taggtagaga aaaagaaata caaagagtaa tacaaatatt
     2881 aagtagaaga actaaaaaca atccagtgtt gataggagac ccaggtgtag ggaaaacagc
     2941 catagctgaa ggtctggcta caaatatagc cttaggaaat gttccagaaa cactaaaaaa
     3001 caaaacttta tactctttag agatgggttc attattggct ggagctaagt atagaggaga
     3061 atttgaagaa agaattaaag aagttgtaga tgaagttgtt aaaaatggaa acataatctt
     3121 atttatagat gagatgcaca caataatagg ggctgggtct actggagaag gctctataga
     3181 tgcttcaaat atattaaagc cagccttagc aagaggagaa atacaagtta taggtgcaac
     3241 tacaattgat gaatatagaa agcatgttga aaaagattct gctctagaaa gaagattcca
     3301 accagttatg gtagatgaac caactaaaga ggactcaata aaaatattag aaggattaag
     3361 agataaatat gaagctcatc ataaagttaa aataactgat gatgctataa aggcagctgt
     3421 agaattatca actagatata tatcagacag atatctgcca gataaagcta tagacttgat
     3481 tgatgaagct gcatcaaaag taagattaaa agaaaataca cctccttcag aaataaaaaa
     3541 attagaacta gaaatagaaa atatagataa agaaaaagaa gaagcagtaa gatgtcaaga
     3601 ctttgagaaa gcagccaaaa taagagatga acaaggtcta cttaaaaaac aattagaaga
     3661 tgttagagaa agatggaaca agtcatctaa acattcagat ttagttgatg gagaagttat
     3721 tgctgaagta gtgggcttat ggacaggaat accagttaat aaaattcttg aagaagaggc
     3781 tgatagactt ttgagacttg aggaaatact gcacaataaa gttataggtc aagaacaagc
     3841 agtaaaatct atttcaaagg caatcagaag gtcaagagca ggtcttaaag accctaatag
     3901 accaattggt tcatttttat ttttaggtcc aacaggagta ggtaaaacag aattatctaa
     3961 ggcattagca gaagtgcaat ttggagatga aaatcaaata attagaatag atatgtctga
     4021 atatatggaa aaacatgctg tgtcaagaat gataggttca cctccaggtt atgtaggtca
     4081 tgatgaaggt ggtcaattaa ctgaaaaagt aagaagaaat ccatattcag ttatcttatt
     4141 tgatgagata gaaaaagctc atccagatgt atttaatatt ctgttacaaa ttctagatga
     4201 tggtagacta actgattcaa aaggaagaac tgtagatttt aagaatacta ttgtaataat
     4261 gacatcaaat gttggagcat ctacaattgg tagacaaaaa actttaggat ttagtatagc
     4321 taaaggagac gaagaagaaa aatctcaata tgaaaaaatg aaagaaaata taatgggtga
     4381 attaaaacaa agattcagac cagagttttt aaacagaata gatgatataa ttgttttcca
     4441 ctcactaaat gaaaatcata tatcaaaaat agtactttta atggcagcga aactacaaga
     4501 gagattaaaa gaaatggata taaaattaga aatgagtgat gaggctgtta aattaatttc
     4561 taaatctgga tttgacttag aatatggagc aagacctctt aaaagagctt tacaaaaaga
     4621 gttggaagat gagctatctg aagcaatctt aaaaggtaat gtgaaaaagg gaagtaatgt
     4681 agtagcaaaa gtcaaagatg aaaaaatagt ttttgaaact aaataattta atagagttgc
     4741 attttaaaat attgcaaata taagttttat aatctaaaca aaattttaag ggcaatgaat
     4801 ctataataat tcattgtcct taattatttt atttaatctt tacatattgt ctttcagata
     4861 tgctaaaatt atagtataga ataaaaaatt aaggatgata aaagtatggc aaaaataaaa
     4921 acaaaatatg tatgtcaatc ttgcggatat gaaacagcta aatggcttgg taaatgtcca
     4981 gaatgtacaa aatggaatac atttgtagaa gaaatagacc aaaaaagcac caaaaaagag
     5041 gtctttataa tagataaatc ttcatctaaa ccagtcagta taaactcaat agagagtaaa
     5101 gaagaagagc ggtttacaac agatataaat gaattagata gagtgcttgg aggaggtata
     5161 gttaaaggct ctttagtgct tgttggtggt gaccctggta taggtaaatc aacactttta
     5221 atccaagtat ctagtaatgt tgccaattta ggaaaaactg ttttatatat aactggagaa
     5281 gaatcagaat ctcaaataaa aatgagagct aaaagattag gtattaactc agaaaaatta
     5341 tatatatttg ctgaaaataa tttaagtata attgaatctt atttagaaag tgtaaatcca
     5401 gaattaataa ttctagactc aattcaaact gtcttcagtc cagaaatatc ctcggcacca
     5461 ggaactgtca gccaaattaa agaaggtact tcaaaattca tgaaaatttc taaaaaaatg
     5521 ggaatttcaa cgttcatagt tgggcatgtt acaaaagaag gttcactagc aggaccaaaa
     5581 ttattagagc atatggttga tactgttcta tattttgaag gagaaagata taatacttat
     5641 agattagtaa gagctgttaa aaatcgattt ggttcaacta atgaacttgg agtatttgaa
     5701 atgagggatt taggattagt agaacttgat aatccatcta aaattttaat ctctgaaaaa
     5761 ccaaaagatg tagcaggttc agtaataata tctactgtag aaggtacgag gccaatgtta
     5821 cttgaattgc aagcattagt ttctcctaca agttttggta taccaaaaag aacatctaca
     5881 ggagtcgatt acaatagagt aggtatgtta ttagcagtat tagaaaaacg tgttggtttg
     5941 caaatacaaa atcaagatgt ctatataaac atagttggag gaataaagat aaatgaaccc
     6001 tcaattgatt tagggatagc catttctgta gcatctagtt ttagaaatat accgatagat
     6061 gaagatatag ctgttacagg agaagtggga cttacaggag aagttagagc agtaagcttt
     6121 atagaaaaga gaatagctga gtgtaaaaaa cttggattta aaaagatagt agttcctaga
     6181 agtaattatg atgttgtaaa agagaccaaa ggcatagaaa tatggccagt agataactta
     6241 agacaagcta taaatatagt acttgggagg aatcaataaa tatggagaat tttctagata
     6301 ataaaaatat gctatatgca ttaaaaatga tatctcctgg aactccactt agattaggtc
     6361 taaacaatgt actaagagct aagactggtg gattaattgt aattgcaaca aacgaagatg
     6421 taatgaaaat agtagatgga ggatttgcta taaatgcaga atattcacca tcatatctat
     6481 atgaattagc taaaatggat ggagctatag ttttaagtgg tgatgtaaag aaaatattat
     6541 ttgctaatgc acaacttata cctgactatt ttatagaaac atcagagaca ggaacaagac
     6601 atagaacagc agaaagagta gcaaaacaaa ctggtgctat agtcatagga atttcacaaa
     6661 gaagaaatgt tataacagtt tatagaggaa atgagaagta tgtagtcgaa gatatatcta
     6721 agatatttac taaggcaaat caggctatac aaactctgga aaaatataag acagtattgg
     6781 accaagctgt aacaaattta aatgccttag agtttaatga tttggtaact atttatgatg
     6841 ttgcattagt catgcaaaag atggaaatgg taatgagagt tacaagtata attgaaaaat
     6901 atgtgataga attgggtgat gaaggaactt tagtaagtat gcaattagaa gaattaatgg
     6961 gtacaaccag aatagaccag aaattaatat tcaaagatta taataaagaa aacacagaaa
     7021 taaaagaact tatgaaaaag gtcaaaaatt taaattcaga agaactaata gaattggtca
     7081 atatggcaaa actattaggg tatagtggtt tttcagaaag tatggatatg cctataaaaa
     7141 caagaggtta taggattctt agcaaaatac atagactacc aacagcaata atagaaaact
     7201 tagtaaatta ttttgaaaac tttcaacaaa ttttagatgc atctattgaa gaattagatg
     7261 aggttgaagg aataggtgaa ataagagcaa catatataaa aaatggactc ataaaaatga
     7321 aacaattagt cttattagat agacacatat gatgaatttt aaatgaattt tactactttt
     7381 ttcttgaata taatatacac tttggtctat aatagataag tataactagg gggtgaagca
     7441 ttgataagga aagtaacaag aatattgttt attttacttg gatttacaat aggtataaca
     7501 acctatttaa cattaatgaa agattttgaa atattaacat ttggaaaaga aacatatggt
     7561 tatatcgcag ctgtagtagc aggtataata atagccattt taggatatct tatagagcca
     7621 tgggttgtaa ataaagtaaa agaaattgct aaaatagtgg ataaagaatt gtcaaaatat
     7681 cctcagactg atatattgtt agggtctatg ggtcttatag taggatttgt tattgcgtat
     7741 ttattaagtg gattagtaaa tagaatacca atagtaggtg gaatactatc cttactatta
     7801 tatttattcc taggatatct tggaatgaaa gttgcgctta aaagtaaaaa tgatttattt
     7861 aatattggaa aactaggaag attagcaaat cctattaagg ataaagataa ggataaagaa
     7921 aataaaaaag aagtaaaagc cattcctcca aaagtattag atacaagtgt aataattgat
     7981 ggaagaatag ctgatatttg taagactggg tttatagaag gaaaacttat tattcctgct
     8041 tttgttttgg aggaactaag acatattgca gattcttcag atgatttaaa aagagtaaga
     8101 ggtagaagag gtctggatat attaaatata attcagaaag aattaaatat agaagttgaa
     8161 ataagtgaga gagactttga tgatatagca gaagttgata gtaaattatt aaagcttgct
     8221 caggttttaa atggaaaagt tgttacaaat gattataact taaataaagt tgctcaattc
     8281 caaggtgttg aagtattaaa tataaatgag ttagctaatg ctataaaacc agttgctata
     8341 ccaggtgaag atatggttgt tcaagtggtt aaagaaggaa aagaggctca acaaggtgtt
     8401 gcatatctag atgatggaac tatgatagtt gttgatggag gaagaaaata tatgaatgag
     8461 actataaaag tactcgttac ttctgttctt caaactcctg caggtagaat gatatttgca
     8521 aaacctaaaa attaatatgt tcaaagaaac ctataatttg ggctgtttat tttaaataga
     8581 tggtccaaat ttatttattt ataacgataa tgtttaatgt attcttaaag atggggattt
     8641 aaatcagaaa gcagggagaa tttatgagaa aaaagattat atctttctta gcaacattta
     8701 tcataatatt aacatcagca agtcagtata gttttgcaga tgatatatca acacgtggaa
     8761 aagtaatatt tatagatatg aatagaacta gtatgagtaa tatgttaaga ataaaatcat
     8821 taagagaaga actagacaat agagggtata ttggcttgat gaatataaga ggcgataaag
     8881 gaagtgatga tagaaggtca tatgctagta tgggtgctgg tggtagagct aatgtggcta
     8941 atgaagaaga tattaatttt gaaagctcga gtaaggatag aaatatagtt tttgaatcag
     9001 ctacaggaaa aagtgcaaaa ggtataaata atttaactat aaataagtct ataaatgaaa
     9061 atttaaactt tggagagtat ggttctgtat tagggtcatt aggacaaagt ttatctgata
     9121 atggattaaa agcatctgta ttgggtaatt cagatataat tgaaaatggt caacttataa
     9181 aaaatagaaa cttatgtcta actgcaatgg atgaatatgg aagaatacct aatggaaatg
     9241 tagatactat aaataaaaaa gacttatcta tgccttatgg aatatcaact gattatgata
     9301 aattgattgt agagactaaa gaagcttaca aaaataatga tgtgattttt gtggagcttg
     9361 gagacacata tagattagat ttatataaac caaatttaaa tgaaaaaaca tatgagagta
     9421 tgaaagataa catagaaaaa aatatagata tatatttaaa aaatatattt tctatggttg
     9481 aagaaaatga cactgtctac atagcaagtg catttccaag tgatttagat tataaaaata
     9541 aaagaagact ttcgccaata ataaaattaa atggagaagg gaaaggtata ttatcatctt
     9601 caactactag aagagaaggt attgtaacaa atttagatgt tggggttgaa atattagaca
     9661 actttaatat aaaaaatcaa aatatggttg gaagaaaata tgaacttatt aacagagatg
     9721 ataataaaga atttttgatg gatgaatatc aaaagatagt atctatttca agcataaggt
     9781 ctacaatact gaatggtttt gtaagtatag ttttcttgtc atggatagtt gctatgattg
     9841 caattttatt tagaaagcat atatcaaaaa attataaaga aacaacattt tttatattaa
     9901 aggaactttt aaaaatagga atagtaatgc cactgtcatt tatgataaca ccaataatga
     9961 atttcaaaac accattagca atatctttag gaataattat aataacactt actatatatc
    10021 ttataagtaa agttttaata aagaacgatt taaaaaacat gctgtttttt actggattaa
    10081 ccatagtaat tatggttatt gatgctgggt ttggctcata tttaatgaaa agcaatgtta
    10141 tgagttatga ttgtataatt ggagctagat attatggagt aggaaatgag tatcaaggtg
    10201 tggcaatcgg tagtgcaata tttacatttg caattttact tacatataaa aatataccaa
    10261 aatggtctat aatagttttt tctttggtta tcttaattac aagtgcatct ccaattatgg
    10321 gagcaaatgt tggaagtgct atttctgaat gtgttgcatt tttactattt atactattga
    10381 tatataatgt aaagatagat ttcaaaaaaa tcgtattatt aggtatagca gtagtatttg
    10441 tattaggagt ttttgtagca atagatatga ttttgggaag taattcacat ttaggcatgt
    10501 ttgtaaaaga aatttacttt aatggaccag gagaaataat acaaacattt agtagaaaga
    10561 tagagatgaa tttaaagcta gctcaaacca gtgcatgggt aaatatactg ttaactggaa
    10621 taggtgtaat tttagttctt atgattaatc agattagata ctttaagcaa ctgatggatg
    10681 agtatccaat tgtatttaaa ggatttatag catcaatagc aggatgttta gtaactcttt
    10741 tagtgaatga ctctgggatt gtgtcatcag ctacagcctt tatttatgta attgtaccta
    10801 tgataacatt aagtataaat cttacagcat taaaagaata agt
//
LOCUS       DAYAOX010000126        10776 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.126, whole genome shotgun sequence.
ACCESSION   DAYAOX010000126 DAYAOX010000000
VERSION     DAYAOX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.126"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(144..476)
                     /locus_tag="ACGI57_002499"
     CDS             complement(144..476)
                     /locus_tag="ACGI57_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFG9717570.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(720..1361)
                     /locus_tag="ACGI57_002500"
     CDS             complement(720..1361)
                     /locus_tag="ACGI57_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFG9717571.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(1342..1635)
                     /locus_tag="ACGI57_002501"
     CDS             complement(1342..1635)
                     /locus_tag="ACGI57_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HFG9717572.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            2016..2087
                     /locus_tag="ACGI57_002502"
                     /pseudo
     CDS             2016..2087
                     /locus_tag="ACGI57_002502"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2258..2392)
                     /locus_tag="ACGI57_002503"
     CDS             complement(2258..2392)
                     /locus_tag="ACGI57_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717573.1"
                     /translation="MNISRRAMKIIELAQKIANKRGVTVQDAWNDAMKEYKEKYEYVA
                     "
     gene            complement(2603..3355)
                     /locus_tag="ACGI57_002504"
     CDS             complement(2603..3355)
                     /locus_tag="ACGI57_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFG9717574.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(3414..5069)
                     /locus_tag="ACGI57_002505"
     CDS             complement(3414..5069)
                     /locus_tag="ACGI57_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9717575.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     YQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(6342..7421)
                     /locus_tag="ACGI57_002506"
     CDS             complement(6342..7421)
                     /locus_tag="ACGI57_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9717576.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIFNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(7505..8161)
                     /locus_tag="ACGI57_002507"
     CDS             complement(7505..8161)
                     /locus_tag="ACGI57_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9717577.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            8522..8689
                     /locus_tag="ACGI57_002508"
     CDS             8522..8689
                     /locus_tag="ACGI57_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717578.1"
                     /translation="MPFIIFPTIEKKSIEEWVNIGANMLCLAQDTTFITYVINQLLSF
                     YNEIVPIQKQH"
     gene            complement(8842..9270)
                     /locus_tag="ACGI57_002509"
     CDS             complement(8842..9270)
                     /locus_tag="ACGI57_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFG9717579.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(9746..10684)
                     /locus_tag="ACGI57_002510"
     CDS             complement(9746..10684)
                     /locus_tag="ACGI57_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HFG9717580.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         3584 a         1915 c         1134 g         4143 t
ORIGIN      
        1 aaaactacta tattatttaa aaatttatat ttatatatgc aattctaaaa acttaataag
       61 aagtgaactt agaaaataaa gcttaatttc aaagacttaa atagactatt attattagtc
      121 taaataaact acgtctatca aaattatata ttaaattctt ctaaaatttt tattgcaaat
      181 cttgcgtttg gatatgtaat agacccgcca ccaattacac ctatttttat agcttctata
      241 atttcatctt tacttgctcc agaattaata caaccctcaa tatgataaca aatacactca
      301 tcacatcggc ttaaaattga aatagctagt cccattaatt ccttattttt tttgcttata
      361 gccccttctg aataagtctt atcatcaaga cttccaaata ctctaaaaaa ctcatctgtt
      421 tcgcttaatt ttttattgta aaatccccta tattctttca tttctctagt attcatcttc
      481 tacctcctat gtttttctta taagaatatc acaccgtact tatattttca atattaaata
      541 caaattttca ttttatgttc attagcctaa tttatattat tttttgaaat ctcaataagt
      601 tatcatataa gtaagatttt ttcagaacta aaatagaaat cttagtaatt tctttattat
      661 ctatctcagt tctgaaatat attattctta tccaaacatt attatagtga ttaattatat
      721 tagttatttt tacctgaaat tttatacatc aaataagaat atataaatgg tagtattaca
      781 attacaaaga ttacaaagat attataaaaa ggaatattaa taaatgcatt aataattaat
      841 aatattccac caataagcca aatccatcca gctaaatggt gtgtcttatt ccaattatct
      901 tcatcattaa gagtccatgg taaaattatt cctactgagt aatttcgttt acatttagga
      961 agatagtttc caaataaaat aactattatt cctaaaatta atcctgtata aaaattagac
     1021 tccaaactaa ctcctgttga atataaaacc atcattattt gtataattac tgaaagtata
     1081 gcaactcccc attttgataa attttgtata acatttgaaa aattagcttt cttaggctca
     1141 ttctcaagtc taaaccatgt atacaaatgg atacatagca ttaacaatgg tacaccaaaa
     1201 gctgctataa cctttggagc ataattatct ggatttccat aattatcaaa atgaattgca
     1261 acttcatttg gtaatctatt ataaaaatat attgatacaa taaatggtat aaaacatatt
     1321 aaagtagata aaataaattg tttatttatc gcttttttca ttatcttttt ctcccttctc
     1381 cataaattgt gaaaaccaaa gcatgacctc ttcgaaaact gaaatattta tttcgtagaa
     1441 aataaagttc ttatactttg tttcaaatat gagtcctgcc ttttttagtt gagatagatg
     1501 ataagaaatt gtagcattcg tcatattaaa tttttcacta atttctcctg ctgacatttt
     1561 attctccctc aacataacca atatttctct tcttataggg tctgaaagag ctttgaatgt
     1621 ttctggaaac cccaacacca tcacctcgct atttagaatt tcatctaaat agaatataac
     1681 ataacatcca ttttatttca atagctattt agaaattttt ctaaatagtt atttctaaat
     1741 atttattgaa aatttttata ttaagattct caatttactt aagtcgcttt tatttaatct
     1801 tactttatct aattttttta acttaacaaa agtgtattgc accataattg ttaaagcaaa
     1861 aacctaatga ttgaggtact tatatatata acaatacttt taattttaaa ttaaaagtag
     1921 ctaaacccaa tttaaaaagt agttaagtcc tatttaaata gaaaagaatg aaacgagaaa
     1981 tatactgtat aaaagtacat aaaaacatct actaattaga tacaactata acagaatata
     2041 tttattatta taataacaaa agaataaaaa aaattaattt agcataacct tgttaaatat
     2101 agatttgaat atgaatttaa agttatgtaa aattaaaaat acaggaatga aaattccatt
     2161 cctgtataaa atctaacttt tgagtctcag tacaatctta ttttgagaca atcttttttg
     2221 ttttatattt tcttatattt aaaatataac gcatatctta tgcaacatat tcatattttt
     2281 ctttatattc tttcatcgca tcattccaag cgtcttgtac tgttacacct cttttattag
     2341 caattttttg tgccagttca attatcttca tagctcttct tgatatgttc ataaacattc
     2401 ctcctacaaa ttaccatata tttcatatat ataaatcaag gttatatttt tatgatatat
     2461 aatccttcta ttgtaagatt acagtttata tgtgtattat atatgaacta tattagaaga
     2521 taattagaat ttttataaag ctatatgatt agattaaata taaaagcttt acaattactt
     2581 ataaatttaa aaagtctata agctaaacta atgtttgata taccaatttt acgaccataa
     2641 ataatgccat tactaaaaat attggtttta tcatctttga acctttcttt atagcaactt
     2701 tagcacctac ataagctccc attatcatca ctaatgcaaa cactatagca attttataat
     2761 ctacttgacc atttatcatg aataataaca atgaagttat attacttgtt aagtttaata
     2821 tttttgtatt tgcagaagca tgtaaaaaat catatccata tatttttata aatccaaaag
     2881 tcaaaaaagt tcctgtacct ggaccaaaaa aaccatcata aaaacccatt ataagagcca
     2941 taagcatccc taatcttaaa tttttcttat taacagcttc aaaattatct tcttgaccta
     3001 aatttttaga aataaaggtg taaatagcca ctattaaaat cataaccaca accaaaacat
     3061 ttaatatctc ttcacttatt gataatacac atctcacacc tagaacacta ccaataatag
     3121 taaatggtac taattttttt aaaaggttgt tattaatttt tccagacttt gcatatctat
     3181 aggcactact tatacatcct gctgatgctg caaatttatt tgtaccaatg gcaagatgta
     3241 ctggcactcc aattgccata agaacaggca tgcttataag tcctcctcct cctgctatag
     3301 aatctacaaa agcagctaaa aaccccccta tacataaaaa aataactgaa aacataaaat
     3361 tcctccatca ttctctttca atttttctta caatatatat tatataatct tatttaatac
     3421 ttattatcca gcatattctt tctatattca tttggagtta tactattaaa cttcttaaat
     3481 atttgtgaaa aataactact gttattaaaa cctactttct tacaaatatc actaatactc
     3541 atgcttctat ctaacagata ttttttagat tcttctatcc taagaagatt tatatactca
     3601 gttattgaaa ctttatttct ttcattaaaa atagaactca aataatttgg acttataaaa
     3661 aaaacatctg atatactatt caaatttata ttttctttat aatatttttt tatatattcg
     3721 tttatatttt caattagatt tcctttatct tttctccaaa atgctctatt cttatcaata
     3781 cactctttgg aaaattcaaa taatacattc cataaatctt catcactgtt tacaagtgat
     3841 atttttttat tatatttatt tcttaaataa gatataagtt ttaaatcctc taaatctcta
     3901 agcactgttc tagacaatac aaatatcatt tcctgaatag tatctctaac atcattatat
     3961 cttaatttgt ttgtatctat tagattataa acaccttcta aattcatatt tttaatttct
     4021 ttttcaatat aaagttctaa ttcaaaggga tattcttttg aagaattatt gaacatatct
     4081 atcggattaa taatatactc ttcttctaat aatctactat ttgctttttt aagtgcctct
     4141 tcaagtctta tattttcttt tctactcttc aatatctcct catcttttat actaagttga
     4201 ttttcaaaaa aattcctctt agctatttcc tgaataaatt cagacatcat taataaattt
     4261 tcttttaata catataatct acttttagga tttaatggaa attcattgta aattttgttt
     4321 atgtatttta tatcaatacc agaactactt gataattcag ataaattttt atctatatca
     4381 atagacttat atactataaa attaccacat tctatatatc caactatatt ttcttctaat
     4441 actataggta tctctaaact ggaaacacca tactcacaat ggtacacttt ttcaaagtta
     4501 tcattattta tgcttttaac aagtttttta gtacactgtt ttatattttt acaaatatcg
     4561 caaaatttat tttctttctt agtctcaaat atttctttat actttaagtc atatacagat
     4621 attctaagct tcaatatatt agatatttga tttgcaagat tttcaattat gtttacatta
     4681 actaactcct gtatattgtt ttcagaaaat actataggta tttgtctcaa actcgaaaat
     4741 ttatgaggaa cataagtaat taatattact aaatcaattt taccagtgtt tataatttca
     4801 tgttctgaat atggtgggca ataaactata gatgtttcct ttatattgac ctttttacca
     4861 tcaattattt gtattccttc tccctgaatc acatataaaa gctgttcttc acttaaatga
     4921 aaatgctttt cttggtagtt tcccggaaaa aatcttacaa ttccagcact aagtctattg
     4981 aattgtgttt ttgatggttc gtgtatccac ataacctctc cccaatcaaa tttttgtact
     5041 tcattatttg actcactaaa atactccata agctcacctc ttagtttttg tctaaatatt
     5101 ttttcttccc ttcttatatt aaaataatat catcatcatt aactttcatt actataaaat
     5161 catcatactc ttttaatact cctgtaattt tagttgcact tagtttacta caattcagtt
     5221 ttttaatata ttgataaatt ctataattac ttttttattt tgtaaaatca gtgttttccc
     5281 aaaataatta tatctataaa tgaaagtatt tttactgctt acatcaatat atgttatcat
     5341 aattttcctt tttattgcat atataaataa ctgtatataa cattgtccaa tactatatag
     5401 aaatacatct aagaaagaat attattaaat tgttccaatt agtattattt ttcttataga
     5461 aataaaaaaa ctgttggagt cttttagaaa aattattttt tacatacctt ttttgcttag
     5521 atagtctatc ttcacagcta ttttgtatat acactcttct agttcatgat tttattttaa
     5581 tatcttgctt atattgctga aataattgaa ttcttttatc tatcatattg tctcttatat
     5641 cacaaacata ttgtttctta tatcacaaaa atgaaagcat tcttatatct tcaatatata
     5701 aacctgcttt ttttaatctt gtaataaata taggatactc tatattctct tcctagaaat
     5761 aactaatacc acttattttt ctattatata gaaagtaata aatctttttc ttaataaaat
     5821 tcaatagtaa gtggaataaa attttatatg aataagaaca ttataaaaat actttataca
     5881 atagtcaata aaagatatta gcttcaaatt atgttaaagt tgtattattt aaagtgaaaa
     5941 aacaaataaa aaagaaataa tagtataagt agaaaaatat acttaaaaca aggcgaagcc
     6001 tctcttgtat ctaaatcaag tagagaatta aatctaaaaa agaaaataaa tataaaagat
     6061 gactcctgta cattacaaga atcatcttat ggtttcataa tcactccatg gtttttaact
     6121 gtctgatata aaggtctcag ttcactcata gcatgaccac cttttccgat tgctgacccc
     6181 gtcacttatg gacaaaatgt ccataagtga tagtcctctc ttaaaaccct ctaaatagat
     6241 atatctcaag caccgcattg aaaccagttg ctggaaacaa actgaagcgt tctggaatag
     6301 taaaagaaat tgccaccata tccacgttac tgtcctagtt gttactttgc taacttcgga
     6361 gcgatttcct ctaacaatac gcagtcaaat tctgcctttt tgctaagaaa tggcagatgg
     6421 tctgcatctg gtataaaaaa aatctgtttg tctggcgcca ctatttcatc aaaaaatttc
     6481 ttcgctggaa gataggggct attatagtca ttttctccca tgatgtagta tacagggacc
     6541 tgatagtcaa ctccataatt tcttatgtca gcttcttcca ctaaaaaccg caatacatca
     6601 ccttgattgt caatcgcatt tggattgaaa atggccgtat tttttaactc tctcaatgaa
     6661 taatacgggg ttgccagtat actgattata ttgcccaaat ccatatttat agaaagccca
     6721 tacttgcttt ggtatgttct tactgtcata agagctgcca aatagcttgg accatatttt
     6781 gctggcggat atggtgcaat agcttccagc gccttgatat ctttttgctt ccctgcagct
     6841 ttcaccattt caagtgtctt ttcatagccc acgcgctcat tgtccaccat attaacggtc
     6901 tgcgcaatcc caatgtacgc gctgtaatcc tccggatagg tttgcacagc catagttccc
     6961 agaactgttc cccagctatg acctataagg ataattttct ccttgttgaa ctcttgcttc
     7021 aaatatgctg tcacctcatg gacgtcttgc agaacacgct ccgcgctcat ggtctgcaaa
     7081 actgctgccg gatcgttcgc aaagtatgtt ttgcctacgt ttcgctgatc ccagttcacc
     7141 acagtaaaat cattttccca ctcatactgg tacagatgta aaaatggcat cattgaatta
     7201 ccaggacccc catgaaccca aaggatgatg gggttgtctg cgttttgtcc ccggatatat
     7261 aacgcctgct gtatgccgcc gatctccacc atttccatgc ggtcgattcc aggagcttct
     7321 attttatggc ttgtacgtat aatcgtgggg gctatcagac gcaaaccggc aaacgccaga
     7381 acaaaaacag caactagtat gcaaagtgtg attaatatca ttttaagtac cttctttcat
     7441 gaattttttt agccatagat atctcctcta acattaaaaa aaatatgttg ctattttagt
     7501 tttgttactc tacctgtccc attggtttcc tttcataaaa aatattaatt gattctataa
     7561 tctcatcttc caggatatca actcctattt gtctcataag aaactgtaag aattgaaacc
     7621 gtttacgaag tgtaggaaag tccccctgaa atacatctgt atcacaccaa aagttaaaga
     7681 gcagcaaaat tacctctgcg cattcatctg ggaactctgt gatgatgctt ctatcctcta
     7741 tgccttctcg taaaacattt gctaatattg gtgctagttt tttcaaatta tcctgtgtaa
     7801 atgccagtat gatatgcgga ctttctatcg cgcttgtaat catgttacta gattctttta
     7861 tcacctcatc cgttaaatgc cgcctaacta aacttctaag cttctctttt gctgtaagtc
     7921 catgcatttc accgagccat tgatttactg tctctattat ctgttcaaag cgcctttcca
     7981 tgaccacatt aaaaatatct tcttttgaac tgaagtgatg aaaaattccg ccctttgaca
     8041 ttcctgaagc atcaacaata ttttgcatac ttgttttgtc atatcctttt tcagcaaaca
     8101 actttgcaga aatggtaatg atattttcaa tagcttgctt tggattgttc tttctagtca
     8161 ttatataact ccttcttatt aaaaaccgac tgttggtttt tattatataa gctattcctg
     8221 cttctgtcaa gtaacattgt gtttatggca aaacaataag gtttggtggt tagacaggta
     8281 ttaattagac taatgcgact ttcccctaga tggaaccctt ggtaaatttg caatctttac
     8341 cggctttgat tatatctgta agcaaggcag gtatggccac acctgccctg tttgcccctc
     8401 ggatattcat tcctatccct cctctcctta gccattacac caaaacgccg ctcattgtct
     8461 ccaatggcgg cgttttaaag atatgatttc tcatgctgct gaaatatgca aaaaatataa
     8521 gatgcccttt attatttttc ctacaataga aaagaaaagt attgaagaat gggttaatat
     8581 tggagcaaat atgctttgtt tggcacaaga tactaccttt atcacttatg ttatcaatca
     8641 attattatct ttttataatg agattgtacc aattcaaaaa caacattaaa ttataaatct
     8701 aattttatat taaaagagac taatatttta tctatctcta ttaaggatat tttcttatgt
     8761 atcataatat tagtctctct tattattata tactgctatc atcattagtt gctatttata
     8821 ctatttttat aatatacaaa tttattcagc taacgttttg cctaattcta tacactcatc
     8881 ttttgcttct tcatttgggt cttccattgc tgtaattcct tctccaccaa taattgtagc
     8941 accagcattt tgcatacgtt cttcccagtc tctcatccat tcacaatttc cccatccata
     9001 tgaaccaaat aatccaatct gtttacctga taccatagat tctaattctg aaacaaatgg
     9061 ctccatatac ccctcttcta gttgctctgc tcccatagat gggcaaccta atgcaaattt
     9121 atcttcttct tttaaatcac ttggtttcag taagctcaca tctaaaactt ctggtgtctt
     9181 accttttaac ttcacacctt cagccacaaa atttgccatt ttttcagtat ttcctgttcc
     9241 actccaataa acaatatata ttttactcat ttttacctcc tgtagtattt tttaattaat
     9301 caatagttag ttatagctaa ctaacatcta aaaaacaaaa gatttcttta tctgaaatct
     9361 ctttattttt ctaaatatag aatatcatgg ttgattttga aattcaatac ttattgataa
     9421 tatttatcat ttatatttta tattcatttt ataatatcaa aacactgtat cgtatttcta
     9481 atctttcata gaaaaatttc taacttatag tacttttgca tctcttataa atcttttata
     9541 tgtataagta catagaaaaa cacacctttt ccagatgtgc tatctataat atcgtatatt
     9601 tatttttcaa ttctaactag ctaatatgtt tataaatttt atcacttaat acctttatac
     9661 attgatgtaa aatcttacac ctaagtaatt tttattattt gtctcgactc tatataatat
     9721 agctatttta tcatcaaatc aaaaattatc ttttgctatt attcttgtat ttataaagat
     9781 ttaaatataa gtatccattt ccttgttccg ttctaggtat tccaagcgct ctcgtacatt
     9841 ttattaattg tgcatacagt tcagcttcat ctaaatctct ttcaaactcc tctcttgccc
     9901 attctttaat tagtgctaat gagcctgata cgtatggtgc actcatactg gtaccactca
     9961 taatagcaag tttattatcc atataagtcg atataatatt tcttcctgga gctaccaaat
    10021 ctattgtagt atttgaatta ctaaactttt caaccaaata gttctcattt attgcaccta
    10081 cttctattac ctcagcataa ctggctggat aactatactc acttgtacta gaatcaccat
    10141 ctccattatt acctgctgca cacactacag aaatattatt ttttactgct tgcatgacag
    10201 cattttttaa attcttatca tctttgtttc ccccaagaga catagatata atatcaacct
    10261 tgttatttac agcaaagtta atagcattaa ttatactttg atatgttcca gtaccatctt
    10321 tatttaatgc ttttgctatt aataatttac aatctggagc tactcccata acttcattat
    10381 tataattaga tgcagctata atacccgcca catgagttcc atgaccatta aaatcctcat
    10441 atatattttt atttccatta ctgtcatcac taaaatttgc accaccaatt atttttcctt
    10501 ttaaaagagg atgagatata tcacaacctg tatctattat accaactaca atatttttac
    10561 cagtataacc ttcatcccac attcccctag cattcatttg ttttattcca tatgggaatt
    10621 tattttttgc acctcttaga ttttcattta tttcatatgg aattagtttc attcttttat
    10681 tcatcatata tcctcctaaa aattttattg cttataaaaa tcatgaattt tattttataa
    10741 taggtttata aatcaaatta aaacctatat ctactt
//
LOCUS       DAYAOX010000127        10737 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.127, whole genome shotgun sequence.
ACCESSION   DAYAOX010000127 DAYAOX010000000
VERSION     DAYAOX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.127"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            160..1359
                     /locus_tag="ACGI57_002511"
     CDS             160..1359
                     /locus_tag="ACGI57_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HFG9717581.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1411..1914
                     /locus_tag="ACGI57_002512"
     CDS             1411..1914
                     /locus_tag="ACGI57_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFG9717582.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            2021..2338
                     /locus_tag="ACGI57_002513"
     CDS             2021..2338
                     /locus_tag="ACGI57_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717583.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            2496..3455
                     /locus_tag="ACGI57_002514"
     CDS             2496..3455
                     /locus_tag="ACGI57_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFG9717584.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            3570..4355
                     /locus_tag="ACGI57_002515"
     CDS             3570..4355
                     /locus_tag="ACGI57_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717585.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            4647..5966
                     /locus_tag="ACGI57_002516"
     CDS             4647..5966
                     /locus_tag="ACGI57_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9717586.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(6041..6469)
                     /locus_tag="ACGI57_002517"
     CDS             complement(6041..6469)
                     /locus_tag="ACGI57_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717587.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(6560..7936)
                     /gene="hydG"
                     /locus_tag="ACGI57_002518"
     CDS             complement(6560..7936)
                     /gene="hydG"
                     /locus_tag="ACGI57_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HFG9717588.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(7937..9022)
                     /gene="hydE"
                     /locus_tag="ACGI57_002519"
     CDS             complement(7937..9022)
                     /gene="hydE"
                     /locus_tag="ACGI57_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HFG9717589.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(9058..10266)
                     /gene="hydF"
                     /locus_tag="ACGI57_002520"
     CDS             complement(9058..10266)
                     /gene="hydF"
                     /locus_tag="ACGI57_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HFG9717590.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTQKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(10281..10532)
                     /locus_tag="ACGI57_002521"
     CDS             complement(10281..10532)
                     /locus_tag="ACGI57_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HFG9717591.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         3965 a         1298 c         1672 g         3802 t
ORIGIN      
        1 aatttgttaa cattaatatc tatatgttaa aatatagtag aagatgttag taaataatag
       61 gatgagttct attttaatac aattaaatta agcttagttt gaaatttgta tctaaaaagt
      121 aaagtaatct agaattaaga aaatggagat agaattataa tggaaaaatt atattataca
      181 aatcagtata taagagattt tacagcagaa ataattgaaa taaaagaagt agaaaataag
      241 tatcatgttt tattagataa gacagctttt tttcctggtg gaggaggtca gtttggagac
      301 ctgggaaaaa tagagaatga tgatgtaata aatgtgtatg aagatggaaa aaaaatatac
      361 catgtattag ataaaaaacc tataaagata cataaggtaa agtgtagtat tgattgggat
      421 agaagagaag atggaatgca tcaacatttt ggacaacatg tattgtctgg atgttttttt
      481 aaaactttta atgcaaatac gacaggtttt catttgggaa atgagtatag tacagtagat
      541 atagaaggta atttagaaga ggataaaatt agagagatag agttgtttgc caatgaaatt
      601 attcgtcaaa atatacaagt agagacatta cttccaacga aaaaagaact aaaaaagatt
      661 tggttaagac gtgatttgcc aaatactgat gaagagataa gagtaataaa aataggtgat
      721 ttagatacga atgcatgttg tggagtgcat ccaaaatcaa ctggtgaatt gagaatgata
      781 aaaataaaga aatgggaaaa acataagtca agtacaagaa tagagtttct agcaggtaaa
      841 agggcagtgg atgaagtatt gaagagggat atatatttga ctaaaatatg tagatattta
      901 agctgtagtg aggaagatgc tataaatggt attgtaaatc taaatagtaa agtggaagag
      961 gctttaatta aaaaaagaag gcttgaagaa gtagtatcta catatcaggt caaagaaatg
     1021 attgaaaaag caaataaggt tggcaatatt tcggtcatta aaaaaattta tgatgatgaa
     1081 gatttgaaat atgtaaataa gatagcaaat aaaattgcag aatatgaaaa tagtattgca
     1141 ttattagctg ttaaatttaa tgataaaata aatttagttt ttgcatgttc taaaaattta
     1201 aaagatatta atatgggagt tttattaaag gatgcaataa atcttataga tggcaagggt
     1261 ggtggaagta aagtattagc tcaaggagga ggtaagaata atggaaattt agaggctaca
     1321 cttgattatg catttatgaa gatagaaaaa actctataaa tatattatat aaaaaacctt
     1381 agtagataaa atagatatgg aggaataagt atggagcaac aaaaggacta catagagtta
     1441 tatgaaaatg aagaactaat agagataaga gaagcacgta tggatgattt ggatacaata
     1501 gcaaaattta attataattt ggctaaagaa actgaaggaa aagaacttga tatggatgtt
     1561 ttaactaaag gtgtaaaagc attgttatta gatgagagaa aaggaaaata tcatgtatat
     1621 acagtatttg ataaagttgt tgcacagatt atgtatacat atgagtggag tgattggaga
     1681 aatggaaatt tcctttggat tcagagtgta tatgtagata aggaatatag aagaaaaggt
     1741 atctttaatt atttatttaa ttatataaaa aatatatgtg ataaagatga aaatatagtt
     1801 ggaatgagat tatatgtaga aaaagaaaac ataaatgcaa aagcaacata tgagtcttta
     1861 aatatgtatg agtgtgatta taatatgtat gaatacgaag tagtacattc gtaatataaa
     1921 tttgtaatac tgttatactg atgttataaa gtattgatta tattgacgat tatatgaaat
     1981 aaactggata ttataattag aatttttagg gggtataaaa atgggcgttt taaaaggaat
     2041 gttttatgta tttttatttt tagatttagt gtcaatattc tgtttctttt tcaataaagg
     2101 aaaaatagca tctaataaaa ttgtgtttaa tgcaatagga gtacttacat ttgtactttg
     2161 ttttatgctt tttagttatt atcctaataa taatcttatt ggaaaattta ttgcttctct
     2221 attttttata tttggagttg caggagttat gttgaaagaa aaaaattttt tatatgctag
     2281 acttctttta actgtagtta tagttttttc tacattaaga ttgttcgtta ttcaataaaa
     2341 tgtatttgta taaaaattta ttaaagtcta cacatttaag tgtaggcttt tttgattttt
     2401 tgtttgaaaa ttaaggaaat tttaaggaaa ttttaaaatc gtcttaaata tttattgata
     2461 tacttaaaat attaatagcg aaaggagact tatcagtgat agaaagtaaa aagtcagaaa
     2521 aattgttact gtcaattata tacataatat ttttggtata tattttaatg attataagaa
     2581 taattttatt tagagatgta ccaatttacg ctattttcaa gggaacgttt aggaatgtaa
     2641 atttaattcc attttataca atatatcaat ttatagtaga tagtaatctt gactttatga
     2701 aagctactgt aaatattata gaaaatatag gtatatttat tcctatggga atttttttac
     2761 ctatagtatg taaaaatcta aataaaaaaa ctataattat agtaattata cttgttagcc
     2821 tagcatttga attaacacaa tatatttttg ctcttggaag tagtgatata gatgatgtta
     2881 ttttaaattc attaggtggc ataattggaa ttaatattta cataaacatg aataaattat
     2941 ttcccaatga tattaacaga tttaaggcta taataggtac tagtttagtt ttaggagtta
     3001 taggattagg agttatttct aaaaattatc ataacttact aacttttaaa tttagacctg
     3061 atagaaaaat atcaaaaata ttaattgaag aaaataggga aataattaaa gatataaata
     3121 aagatacagt agatatagtt gggacatttg agagctttaa aaaaggaata ataactatta
     3181 aagcaggttc taataacaaa gtaaagagac cagagaattt aattgattct gatggaaata
     3241 taagaattta tttaaatgaa aatacaaaat tagtatctta tataattacc aaagaatcaa
     3301 aaatggatat agttaaatat gaggaatttg atgtgaagaa tttagattta ttaaggagat
     3361 atgatactat taatgtttgg attgataaag aaaatcagcc aaaggataaa catggtatta
     3421 tggctagtaa attattaatt ggattgtatg aataaaatat taaacaagaa taaaagaata
     3481 acaaatataa aaaacgaaaa aactatttct ataattattt ttacgttgtg cataaaataa
     3541 gaacattagt gaaggagtag agataaatta tggagataat aaaatatatc ttgtactttt
     3601 ttatatattc atttttagga tggacagtag aaagtatagg atgttctata gcaagcaaaa
     3661 gaataatcaa cagaggtttt ttaaatggtc caatatgtcc tgtttatggt tttggagcgg
     3721 taattgtgat atcactattg ggaagattta ataatgtagt aatagtattt ctattaggaa
     3781 tgattttaac tacgatacta gaatatttta caggatttat tttagaaacc ttatttcatg
     3841 ctaaatggtg ggattattct gacagaaaat tcaatataaa gggtagagta tgtttaaaaa
     3901 atgcaatata ctttggtgta atgtctgttt taataataag atttatacat ccatttataa
     3961 aatattttgt aagtataatt ccatatagaa tattaatttc aatggctata attacaacac
     4021 tttggactat acttgatttg attgttacta ttataacact taaaaaatta gatataaagt
     4081 taaacttact agacgatata attacagacc ttaatgatat aaatgtgaaa ttggataaat
     4141 ttgatagagg agaaatacaa gctttattta aaacaataaa taacgacgaa ttagaagtta
     4201 gagaaaaaat aaacaaaata aatagtaagt tggaccgtat agagtctaat attattttac
     4261 aaaaaagagt tataaaagct tttccacata taaaacataa aaaacaacaa gagcaactag
     4321 aacattttaa aaaaattatt aatgagaaaa ggtaattata caatttaatg aagacgaata
     4381 gaataatggt atttataaga tgattttcag aggactctat aaaaatagaa tagagtcctt
     4441 tattttttga aaaataatgt ttatttgata aaaataaaat atattgtttc tgaataacaa
     4501 atattttgaa aattataaaa acatatattg acaatttgtg tcaaaaatac taacattaag
     4561 tatataaaga attttatatg agaatatctt tataaatcgc aataatttta aaacataaat
     4621 ttatttaaag aggggatgat ttgccaatga gcgagataac tagtcaaatg ataagtacag
     4681 aagaaaaaaa atatgttgct aaaactcaaa agatacctta ttatccagta gcatttaaat
     4741 caggtgatgg tgcaatgcta tatgattatg aaggtaatga atatgtagat tttttagcaa
     4801 gtgcaggctc tgcaaatgta ggtcatggta ataaggaaat ctcacaagca gttaaagaac
     4861 aaatggatga tataacacaa tatacacttg catatttcca tagtgatcct cctgtaaaat
     4921 tagctgagaa gcttgtggaa atagcccctg gagataatga taaaaaagta ctatacagtg
     4981 caactggttc tgcttgtata gatgctgcta taaaattagc aagaggatat actggaagaa
     5041 caaagataat atctatgtgt gaatcttatc atggaagtac ttatggagct atatctatat
     5101 ctgctttaag tacaaatatg agaagaaaaa tgggtccatt gttaccagaa gtttatcact
     5161 tccattatcc tgataaaaat agaacagcta aagaatgttt agatgagata gaatatgctt
     5221 ttgcacacta tctaccagca gaagaggtag ctgcaatatt tatagagcca attgcaggag
     5281 atgcaggaat aatagtgcct ccagtagagt gggtacaagg tttaagtaaa atatgtaaag
     5341 aaaatggaat attacttgta agtgatgaaa tacaacaagg tatgggaaga acaggtaagt
     5401 ggtttggtat agaaaatttt ggtgttgaag cagaccttat agttcttgga aaatctgttg
     5461 gaggtggatt acctctagga gcagttgttg gaagaacaga aataatgcaa agtcttgatg
     5521 caccagcaca tttatttact ttagctggaa acacaacagt atgtgtggct gctttaaaat
     5581 ctatagagat aattgaaaaa gaaaacttac ttcaaaagag tatagaaatg ggagattata
     5641 ttaaagcagg atttgaaaaa ctaaaagaaa aatacgatat aattggagaa ataagaggca
     5701 taggattatc tataggagtt gatatagtta aaggaaaagg ttctaatgaa aaacatccag
     5761 atgctacagc taagatatgt tatagatgta tacaaactgg attaataatg atattcttag
     5821 gacaatctac tttaagagtg caaccacctc tagtaataac taaggagcaa gtagataaag
     5881 caatgaacat catagattct gcaatagatg attatttaaa tggtagaatt ggtgatgaag
     5941 tatatgaagt aactcaaggt tggtaaaata aatattaaat gaaaatagga gttatctcaa
     6001 ttttagaaaa ttatttctga aatgagataa ctcttttaag ttacccacaa ataagaaaat
     6061 tgtttgattc attaaagact atatttcgga tgtcgttttt gtgagtatca gagacagtat
     6121 tacgagaata attaccttta ttcgtactat ctgttaattt agttataatt aagatagcta
     6181 aaagaataag aattactgtg tataaagcaa taacatatgg ttttctttta tatttgtatt
     6241 tgttattgaa aaaagaatgg ccataattat ttttatttac tttagatgta tttgtattct
     6301 ttttcttgtt acctttcttt ttatcatcct tttttctatt tttagggtaa ggaatgactt
     6361 tgtgtgtatt atcaagtttg atatttttat ttttttgaat attgttttcc aggctagcct
     6421 ttttttctcc aaattgaata gttttattgg tattgttgtt atctttcaat gttaatctcc
     6481 ctcccttaaa ctttcccttt agataatatt actactaacc tatggaatat acaataaata
     6541 atatataagt aataaaattt tataggtata aatctctttc tccacgctct aaggcagtca
     6601 tattgttaga tactatgttt cttatatctt ctctttctat cttacctatt tcttctgcga
     6661 taagtttttt acccataatt ttaagttctt catctccata atcgattaaa aattcattta
     6721 atgtagtaag agcatttggt gtacatacat tatggatttg tcgactttta gctaagctca
     6781 tgaatctatc gccagttcta cctttacgat aacaagctgt acaataacta ggtatatatc
     6841 catcagtaat cagttcttta agcacctcaa cagggcttct atggtctcca acttcaaatt
     6901 gttctgtatt gtcaccatct tcatatgctt tatatcctcc aacacctgtt agagaacctg
     6961 cactaatttg tgatacacca tattttagta actcatttct catttcagca gtttctctag
     7021 tggacattat gattccagta aaaggaacag ccaatctagt tatagctact atttttttga
     7081 acatatcgtc agaaactaag tgaggaaaat cttctaagct cataccttca gcttttttca
     7141 atctagggaa agatatagta tggaaaccta caccaaattt ttcctctaag tgttcgttgt
     7201 gcatcataag tcctagaact tcaaatttag ggtcagataa accaaataaa actcctgcac
     7261 caacatcatc aacaccagct tccatagctc tatcaaaagc tgttaaatgg taataatagt
     7321 cattttttat agattgacca tgcattttta taaatgttgg tttgtgataa gtttcttgga
     7381 ataatatata agttcctatt cctttttctt ttagtaattt atactcatct acacttgtag
     7441 ctgctatgtt aacgtttacc cttcttatat ttccgttttc attatatgtt gaataaattg
     7501 catctaagca atctaagatg taatttatat cacaattttt agggtcttca ccagcttcta
     7561 gagcaagtct tttatgaccc attttttcta atattttaac ctcttccttt atctcttcca
     7621 tggtaagttt ttttctttta aatttgttgc atttactaaa accgcaataa acacattcat
     7681 ttacacagta gtttgataca taaagagggg caaataaaac cactctatta ccatatattt
     7741 cattttttat ttgcccagct atactaaata atctatctag ttgtttttta tcttctactt
     7801 caagaagtgt agctatatct ttataactaa gtttttccct tctatcagcc ttatcaagta
     7861 ccttttcaat atcatcagaa gttgaatttt ttgcatcttc taataaagaa ttaataagtt
     7921 catggtttat gaacatttac aaacgcctcc attcaatatt atcaccgcga gaatagtcaa
     7981 cttcaagtcc taatgacttg atgttttctt ctaaactttg gtgatattca caagcttctt
     8041 gaccagtaaa ggctttgtta tcgtaaagag aatattgttt tctaaattct tgaggagaaa
     8101 ggtttggcat aattacattg caaccagcta aaagacctgc atttctacca ctagggttta
     8161 tagatgccaa ggctgttgta gcgggtaata aaacttttgg aagtagaagt cttgtaattg
     8221 aaagcataag taatgtcttc tctaaatctc catgtttata atccttaaaa attgtatcat
     8281 ggtgtggaat aaaaggtcct attccaacca tatgaggatt taactctttt aggtataata
     8341 aatcacgaac aaggtcttcg ttgttctgaa atggtgattc aaccataaat ccagcaccta
     8401 cctgataacc aatttcttta aggtttttta ggcattcttt tctagttcta agctcaatgt
     8461 ttggcggatg taatttttta taatgactgt ctgtagcagt ttcatgtctt aaaagatatc
     8521 tatctgcacc acataaaaag tattttttat acgaatcata actcttttct cctagtgata
     8581 gagttatggc acaatcctca tatttatttt ttatacttga aacaatttca catattttat
     8641 catcagtaaa atatgcatct tcaccacctt gcaaaacgaa tgttctgtat cctattttat
     8701 aacctatatc acaacattca agtatttcat caagtgaaag cctgtatctt atgatatttt
     8761 tattagaaga ttgtattcca caatagtagc aattattttt acaatagttt gtaagctcta
     8821 taagtcctct caagtagact ctatttccat aatgtttatt tctcatgtcg ctagcttgtt
     8881 tataaagata gtttcttaaa ttttgttcga agttatattc ctcattgtcg atattttcta
     8941 ttaaatatag tagttcatca aaacttaatg aattaatgct gtaaagttta tctataaagt
     9001 atttaatctt atttttagtc atattcaaat atgtaattat taagtatagt taaattttta
     9061 ataattgaaa agttcaagtg atctttccaa aatacctttt acatatgcta ttaagacacc
     9121 atagtttaca ataggtacat tgaaagattt tgccttttct attctagata acattccagc
     9181 tctattcatc atacaagctc cacagtgaac tacaagggca tatttattta tatcttcagt
     9241 gaaggatact ccagaactaa attcaaaagt aatgtttttg ccagttttct ttctaatcat
     9301 attaggaatt tttactgttc caatatcatc agtttgtcta tgatgggtac atccttcggc
     9361 catcaagatt ttatcaccat cttttaaatt ttctaaagca taagctccat taatcaattc
     9421 tttaaggtct cccttttgtc tagcaaacaa tatagaaaaa gatgtaagag gaatatcttt
     9481 ttgtgtatct ttatctactt gtggaaatac ttgagagtca gtaataacaa gttttggttt
     9541 tttgcctaaa ttagatagag tttcttttaa actatcttct tttgtaacta tagaaattgc
     9601 accattatcc aaaatatctc ttataacttg ctgttgagga agtataagtc taccttttgg
     9661 agctgcttta tctattggaa caactaaaac aactaaatca tttggttcaa ttaaatctga
     9721 taccaatttg aactcagtgc tatccttagg caaaactttt attatttcat tttttaaatt
     9781 ttcaattcca attttatctg ttgatgagac agaaacaact ggacatttaa ctttattttt
     9841 agttaagttt aaaatttctg attgattctt aatagtatca attttgttaa gaattagaat
     9901 atgagggata ttcttatcat taaatttttc aattagtgac aaatcttctt gtgatatacc
     9961 aatttggcaa tccactacta gtaaagctat atctgtcttt tctaaaacat ctagtgattt
    10021 acctattcta agttcaccaa gttcaccaac atcatcaaga ccagcagtat ctataagaac
    10081 acaaggtcct ataggaagga tttccattgg tctaaatact gggtctgtag tagttccagc
    10141 aatatctgag actattgcag cactttgatt tgttatagca tttattatgc tagatttacc
    10201 tgcatttctt ttaccaaaga gacctatatg tactcttacc gattgtggag tagaatttag
    10261 actcatttgt actcaccacc ttaaacttct ttttttgata tactagtttt tacagataca
    10321 tgaggtatgt tgcccaattt accagtcaaa ctattaatag tgtccatttc gccaacaact
    10381 gctatacata cgattgagac accttcttct tcgcaaggaa tgcccattct tcctttaata
    10441 atacctttaa aacctgatac aacctcattg aatttaaatt gacattcttt tggttcttcc
    10501 aaaatagcac ttataactgc aacctttttc ataaatatcc tcctaactca gaaaatatgt
    10561 cttaaacaaa tccaaaatca aaaaactttt ctttgtaaag aaaagtttct acaaaagaaa
    10621 agttattaat tacacaagta taatgtgcta tattaaacta tttccttaca gattagataa
    10681 ctcttttctg gtagggttag cttgtaaaaa tttaatttaa caacaagtaa ctgaaga
//
LOCUS       DAYAOX010000128        10637 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.128, whole genome shotgun sequence.
ACCESSION   DAYAOX010000128 DAYAOX010000000
VERSION     DAYAOX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.128"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            575..2005
                     /locus_tag="ACGI57_002522"
     CDS             575..2005
                     /locus_tag="ACGI57_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9717592.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            2108..3211
                     /gene="hisC"
                     /locus_tag="ACGI57_002523"
     CDS             2108..3211
                     /gene="hisC"
                     /locus_tag="ACGI57_002523"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFG9717593.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            3244..3462
                     /locus_tag="ACGI57_002524"
     CDS             3244..3462
                     /locus_tag="ACGI57_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9717594.1"
                     /translation="MNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAHVEVDES
                     KCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3479..4528
                     /gene="vorB"
                     /locus_tag="ACGI57_002525"
     CDS             3479..4528
                     /gene="vorB"
                     /locus_tag="ACGI57_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFG9717595.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4530..5270
                     /locus_tag="ACGI57_002526"
     CDS             4530..5270
                     /locus_tag="ACGI57_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFG9717596.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5273..5812
                     /locus_tag="ACGI57_002527"
     CDS             5273..5812
                     /locus_tag="ACGI57_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFG9717597.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5936..8035
                     /locus_tag="ACGI57_002528"
     CDS             5936..8035
                     /locus_tag="ACGI57_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HFG9717598.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMEKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8302..8817)
                     /locus_tag="ACGI57_002529"
     CDS             complement(8302..8817)
                     /locus_tag="ACGI57_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HFG9717599.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8909..10120)
                     /locus_tag="ACGI57_002530"
     CDS             complement(8909..10120)
                     /locus_tag="ACGI57_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9717600.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKFLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         4047 a         1272 c         1913 g         3405 t
ORIGIN      
        1 tgttttattg gttttagatt aatatagcta taataattta tgatattaga tggaaattac
       61 gtaaaaatag aaaattgaaa attctgaaat ttttgcatat tgatatttat ctcatagatt
      121 cttttttgta attgtaacac gcttttattt ttaaatcaat agtacatgta taaaaataaa
      181 aggaatttaa ataagaaaaa gatattttta atatctggat gtagtataga aatatcttga
      241 ataaattatt cttgaataaa attataaaaa attaaaatca ctagaaatat gataaaataa
      301 tttaaaatac gcatttcaat gacatttcaa aacaagaaaa taataaaaaa caaacctaaa
      361 ttaagtctaa atagcttttt agttaatttt agtagtaaaa aataaaactt aaaaagtgtt
      421 tgacaattat gaattttgag aatataatta aattaaaatt atacacatat aaaaatatac
      481 tcctgaatca agaatgagat atttaagatt caaaatatat attattatac gtgaataata
      541 aaaagtaagt aaattttata agggaggggt agatgtgagt agtcaagaaa gcaaaccaaa
      601 aggaaatctt atatcaaatt ttagagatga tatgagagac cctaagaaaa gagcaatata
      661 cacatattca agttctataa atttagcgta caattttata ggttactttg tatcaactgt
      721 tacaaatatt tttttaactg atgtggcaaa acttggtgta attatggcag gtggtatgca
      781 aattatacaa tctttcataa aaattgctgt agctcctata acaggtacag tatttgacaa
      841 acaaccattc aaaaaaggaa agtattatcc atggcttaga tatgcaccag cagcgttagc
      901 attaacatat ggaatatttt tcatagttcc attaatgaaa ataagtccag cattactttt
      961 acctttgatg acagctttat tattaatagc atcttgtgct cagcaagtag ttttcactat
     1021 aatttatgct atttaccctg tagttgctaa gacacctaga gaccgagtta taggctcaac
     1081 tactacgaat atatttaaag aagttggtaa attcttagta ggtttttcat atccgttatt
     1141 attagtttta tttgcagatg tattaggtgg agaaggaatg ggttatttag gtacctactt
     1201 attctttgca gcaggatgcc ttttagtatt ttggttttct tcaacagaga taataaaatc
     1261 tggagcagaa gaagaagttc ttcaaaaaga agagaagcca aagattaaag caagtgaaat
     1321 ggcaaaagct ttattcacta atgtaccact tatgatggca ttttgtttag aatttcttat
     1381 atgtataaga tctataggat taggaccttt ggctccatat tattttaaat atgtaataga
     1441 agatgaacgt ggattagcag tatttttatc aatcatgcct ttggtaagtg tagcattcat
     1501 gtttttcgca ccagtgttta taaaaatctg tagagagcaa aaactagctt caataatttc
     1561 tttttctata tgtgcactat gccacttatt ggttgcatgg actccttggg ggaaaactac
     1621 tttaggagta actatgttgt tggcaattgg tggaggtttt tcaaatgtag tatcaataat
     1681 aaatcttaat ttctttgccg gttcttgtga ttatgggcat tggaaatcag gaaaagactt
     1741 acctggctta agtatgtcac tatatccagt tgcaatacaa gtaggagtat tacttgcaac
     1801 tactataaga acagttttaa tgaactcaat gggatatcaa gctgatatgg tagtcacaga
     1861 agcagttaaa tcaggtttta taaatatgat ttcttattct atggcaatac ctttaataat
     1921 agctgttgtt atagcaatac tatatccagt aagtgacaag aaattaaacc aaataagaga
     1981 agagttaaat caaagaaatg cttagttaaa agaaagttga gtagatggtt aatttaagct
     2041 attaaagtaa gtagacgcaa tagcgtttat ataaatgaaa atacatatac agagggggct
     2101 tttaaaaatg atagaaaaat tattgagaaa taccgtaaaa gaattacatc aatatgttcc
     2161 aggtgaacca atagaaaaag ttaaggaaaa atatggtgta aaagaaataa taaaattagc
     2221 ttcaaatgaa aatcctctag gaccatcacc aaaagcaata gaagctatga ttgaaatgtt
     2281 aaaacaaggt caactttatc cagagccaga agctaacgaa cttagaagaa aattagctga
     2341 aaagctagat ttaaaaccag aaaattttat tgttgctaat ggtgcagata acgttatcac
     2401 tctaataggt gaggctttta taaatagagg agatgaagtt atatattgta acccaacatt
     2461 tccatcatat agaactgcaa ctataaaaaa tgaagggata ccagtagaag tacctttaac
     2521 agaagattat aagtatgatt tacaaggtat attagataag attacagata aaacaaaatt
     2581 aatatgtgta tgtaacccta ataatccaac aggaacaatt gtggatgata aagagttaga
     2641 agaattttta aagaaagtcc ctgaaaatat tataactata ttggatgaag catatataga
     2701 atttttaaca gttcctaatt atgtggatgg acttaagtat gtaagggaaa attataacgt
     2761 tatagtaaca agaactttct ctaaaatata tggtcttgca gggcttagag taggatatgg
     2821 tattgctaaa gatgagataa taagaacttt atttacagta aaagagcctt tctctgcaaa
     2881 tcgtgttgct ataagtggag caacagcagc acttgatgat gaagagttta taaaagaaac
     2941 atatgaatta aatagagttg gaatggctta ctttaaagaa gagtttacaa aaatgggatt
     3001 tgatgttgta gattcacaat caaatttcct ttatgtagat atgaaaacag atatacctaa
     3061 gttatttgaa gacttgaaaa aaagagggtt tgtaataaga ccaagtgcta cacatgcacg
     3121 tgtaagtatt ggaactatgg aagaaaatag gaaatttgta gcagttttaa aggaaatatt
     3181 aaatattaaa ggagagccaa gtattggata atagatagaa atattatgaa acggggtgta
     3241 gatatgaact taaaaataaa cgcagagaga tgtaaaagtt gtgaatactg cgttatatct
     3301 tgtaaaaaag gagcattaaa aatatcaagt aagataaata aagaaggtta tgctcatgta
     3361 gaagttgatg agagtaaatg catactttgt ggaatttgct atcaggtgtg tccagataat
     3421 gtttttgaaa taattaaaga agaagcatgt caagaggctt agaaggagtt gggataatat
     3481 gaaaaagatg tggaagggga accacgccat tgcagaagca gccctaagag gaggttgtga
     3541 attttatgca ggatatccaa ttacaccaca aacagaggta atggaatttt tatcacatag
     3601 aatgtctgaa cttggaagaa cttttataca gtccgaaaat gagatggctg ctatatatat
     3661 ggtatatgga gcatatgcaa gtggaatgag gtctatgacg agttcttcag gaccaggaat
     3721 atctttaaaa caagaaggga tttcttactt atgtgcaaat cattaccctt gtgtaatcgt
     3781 aaatgtacag agatgggggc ctggtcttgg ctcattggac tcagcacaga cagactatct
     3841 aagagatact agagggggag gaaatggaga ttatcatttg atagtttatg caccaaacag
     3901 tattcaagag actgtagatt tgatgtataa ctcatttgat gtagcggaga agtacagagt
     3961 gcctgtcgaa attttgacag aagcagcttt aggtcaaatg atggaaccag tagaatttcc
     4021 agagtttaag aagagagaag gagatttagg ttggacttat gatggaagta atagagacca
     4081 tgcaaaggtt gcagataacc aaaagccaac tttttgtatg gagaaacgta tgagaataac
     4141 tgaaaatgaa caacaatggg aagattatca agtagaagat gcagaatatg tgtttgtagc
     4201 gtttggtata ccatcaagaa caactatgaa tgctgttaga agacttagag aacaaggtga
     4261 aaaagttgga ataataagac caataacagt atggcctttc ccatataaag catttgaaaa
     4321 ggtaagtaaa gatgtaaaag gatttatctc agtagaatca acagacacag gtcaattagt
     4381 agaagatgtt gctttagcct caaagaaagt atgtaaagaa aatgtacctg tgtatggttt
     4441 atttagtgga aatcatatac caaaaacatt gcaagtaatg gacacttata gcaaaataaa
     4501 atctggcgaa atcaaggagg tgttctaata tggctacaga aaaattaaca cctagcgttg
     4561 tttcaatacc aaacaagttt tgcccaggtt gtggtcatgg aatagtaaat cgtataatag
     4621 cagaggtaat agaagaacat ggatatgaaa aaaatcatgt attaacatta ggagttggtt
     4681 gtgtctgcaa tatgaatttt agctggaatg gtgataagat gcaaactgca catggtagag
     4741 cttcatctac agctatagga gttaaagtag cacttccaga tacattagta atgacttacc
     4801 aaggtgatgg agatgcttat gttataggtc tttctgaaac tttaaataca gcatatagaa
     4861 atcataatgt gactgtattt gtaataaaca ataataattt tgcaatgact ggtggacaaa
     4921 tgtcatggac cactatgccg ggtcaagtca caacaacaag tgtaaatgga agagatataa
     4981 agactactgg tagtccactg aaagttcctg agatggtggc aaacttcttt gatgtagcat
     5041 atgtagcaag aggttcagtt catagcccaa aggaaataat taatctaaaa aaatatataa
     5101 aaaatgcaat agaagctcaa ttaaatggag agggatattc cttagttgaa atattagcac
     5161 cttgcccaac taactggggt gtatcactag agaaatctat caaatggatg gaagaagaaa
     5221 tagtgccata ttatgctctt ggcgaattta agcaaaggga tggtgaatag ttatggaaaa
     5281 agagctagta tttgcagggt ttggtggtca aggagtttta actcttggtc tgattgtagc
     5341 tgagtcagca cttgaattgg ggaaacaagt aacttggatg cctgcatatg gtccaactat
     5401 gagaggtgga aaagcctata gtgtagtaaa gttttctgat gagagtatag gtgggcctga
     5461 tatggaagaa atagatgttt tagtagcaat gaataaacca tctttagatt atataaattt
     5521 agttaaagaa ggtggaacag tagttattaa tacatctgca atagatgaga atgtaacatt
     5581 aagagaagat ataagtgtag ttaaaataaa ttgtcaggaa ttagctcaaa aagtaaacaa
     5641 tccaaaagca gcaaatatag tagtacttgg agcattaatt gctaaaactg ggttatttga
     5701 aaaagaatta gctttaaaaa ctatgtgtga tttctttgag gaaaaaggaa aaggaaagtt
     5761 taatgttcaa aatgaagcag cattcatgga aggctataat aacgccattt gataaattac
     5821 ccttatctta agtgatttat ctcaagattt aataatcttt agtgccaaga gaaacttcat
     5881 cttgagataa aattcactta aaatcacaac tataaacata taggagcgtg aatttatgag
     5941 tttggaaaaa cttattagac caaaaagtat tgctatagta ggagtgactg ataaacttgg
     6001 ttttggaaga agtgcagcac ttagtatagt aaaaagtaag gaaacagata gagtatatta
     6061 tgttaatccc aaaagagaag aattgtttgg gagaaaatgt tataagacga tacaagaagt
     6121 tccagaagta gtagactgtg tagtagtatg tacgcctaga aatgtagttc catctgtatt
     6181 aaaagattct ggagagttag gaacaaaggc agcagtggta tatgctagtg gatttgcaga
     6241 ggaaggtaca gaggaaggta cagatttaga aaatcaactt atagaaatat caaatacata
     6301 cgatatgaaa atattgggtc caaactgcat ggggttgcta aattgtatag ataaggttaa
     6361 tctatgggca ggaggttcta aatgggattt agatactaaa aaacctggta taggaattgt
     6421 aggtcaaagt ggatttataa ctgctgaaat tgtttcaagt gattatttta atatatctta
     6481 tggtttttca acaggaaatg gaaatatagt tacactagaa gatgcagtag atttccttgt
     6541 agatgatgat tatgcatcag taatagcaat ttaccttgaa ggtttaaaaa atcctcaaaa
     6601 atttatagat gctttaaaaa gggcagcaca aaaaagaaag ccagtaataa tattgaaatc
     6661 aggaaggtca gaaaaaggtg ctatttctgc tgcttctcat acaggaaatt tagctggttc
     6721 gagtaaagct tttgaaagta tttttgaaaa atatggagta atttctgtgg aaaatttaga
     6781 acagtttatg tgtttagcac aagcttttag tgttttagat ggtaatttac ctgcaaattc
     6841 taattttgca gcaataaact tctcaggagg agaaaatact atttgtgcag atttagcaga
     6901 agaaaatggt gtagaacttg cagaaatatc ttctgaaact aaagaagaaa tggaaaaata
     6961 tcttccagga tttgcaacac ctaaaaatcc actagatgct acaacagcat tattccatga
     7021 aaaggatatg atagtaggtt tattacatac ctttgaaaag gatgatagtg ttggatttac
     7081 tatggtaggt gcaaatataa gagatgaaga aaatgaaatg catgaaaccc tttgtaaagc
     7141 tgtaagtgaa gctagagaac aaggattgaa aaaacctgtg tttgcagttc ctacacttga
     7201 aggaaataga tatttagatt ataggactag attagaagat aatcaagtgc ctataatgtc
     7261 atcagttgga actacattta cttgttttaa taaaatggct aagtttatag attatgatta
     7321 ttcaaaaaga actttagaat ttaaagctgt taagaaaaga gagtcaaata atgtagtagc
     7381 attatcagaa ttagatagta agattgagat gaaaaaatat ggagtaccag ttccaggaca
     7441 aggtaatgca aaaagtattg atgaattaga tgaaatctta aaatatatta agtatcctgt
     7501 tgttttaaaa ataaattctt ctgaaatatt gcacaaatct gatgttggag gagttaaaat
     7561 aggaattaag aatagagatg aggcagtaga tgcatataat gagattttaa ctaatgtaaa
     7621 aaaagcaaaa ccagatgcta atattgatgg tatattagtt caagaaatgg tagaaagtgg
     7681 tatagaaatt attattggta taactaatga tgaccaattt ggaccaatgt tattagtcgg
     7741 tttaggagga gtatttgtag aggtatttaa agatactaca ttatatccat tacctataaa
     7801 tcatgatgaa gctattatga tgcttaaaaa actaaaatca tttaagcttt taaatggcta
     7861 tagaggaagt gagccatgtg atattgatgc tcttgcagat atgatggtca agttaggaaa
     7921 atatgcttat gaaaataagg atgaagtcaa ggaaatagac ttaaatccag tgtttgtata
     7981 tcctaaagga aaaggcgtat gtgcagtaga tgccttaata gttaaatata aatagtttat
     8041 aagttttggt attcaaccat aattaaagca ccaactgaaa atcacatttt gtaagttaga
     8101 tagtgtgatt ttcagtcacc ccaaatttaa tttaaaaata ttttaaaatg aaagtatagt
     8161 tttaagtttc attgtgaaat agcactgtat aaacaataaa atactacgta taaaacgaat
     8221 aataaaaggt gttttaatga taagaatcaa tcttgatttt cattaaaaca ccttctttat
     8281 tatattaaaa atttttaaac gttatattgt cacaggtggg tttgttggag ctgcatatgt
     8341 acgattagca aattcttggt caacaaagaa tagcccaccc tctccagcaa gtttaacacg
     8401 ttttatattg tcttcacaat ttgtttcttc ttctgcttgt tcagatataa accaaagtaa
     8461 gaattgagaa gttctaaagt ctgattcaga gtttgctaca gctgctaagt tgtttattaa
     8521 agctgtaacc tttttttcat gagctaacgt acgctcaaga atatccattg cagaagtaaa
     8581 gtctgaatct ggcattggga tagcatcaag ctgaaccttt cctcctacat tatgtaagta
     8641 tttgtaaaaa aacatagcgt ggtctgtctc ttctttatat tgaacataaa accaattaga
     8701 aaatccttta agtccttctg cttcaaggta agaagacata gaaagataaa gatacgcaga
     8761 atataattca tggttaatct gctcattaag taatttttcc attttagctg aaatcatttt
     8821 tatacctcca aattttaaat gctcttataa aatataataa tattaaatgg aaaaatattc
     8881 aataaaagtg tttaaatatt ttacatattt aataaaaatt tatactacca ttaaaaaaat
     8941 tataacttac aatggctata gagccaatta atggtgcatt tgctccaaaa tttgaatgcc
     9001 ttacacttat ttttttatat ttagaaaaac ttgctgaagc agtcaatttt tttaataatg
     9061 tattttctat aaatgtacca ggtatattaa agtcatagcc tattattata gaactacaat
     9121 caatcaatgt aagtgtatta actaatgcat atgatacgta tgtacaaaat tcatcaagca
     9181 aaaacatggc aagactatct ttattatttg cctcatctat taacttgact aaagaaaaat
     9241 ctttacagtt aattaatggg gagttaggat aaatatggct caatgattca attcttttaa
     9301 ttagattact gacacttgta taataatcta aacatccatt gttaccacaa ctgcacaatg
     9361 gtccactaaa attaattgat gtatgtccta tttctccact ttgtcctagg ttgccagtat
     9421 gaagtttatt ttctagaact agaccagctc caataccatt cattatgtgt aggtatatat
     9481 aatttgatat atttttacct aaaccataca ttttttctgc taatgcaccc gaattagcat
     9541 catttatcaa aaatattggc aaatcagaca tttcttttat aaaatctact atatttaggt
     9601 ttgatacatt tccaaaatct gggggattta gtatggtacc atttatatca tctactggac
     9661 ctattgaaga aataccaatt ccaattatat ctctcttgtt gattttcatt attttagtaa
     9721 acattctttt tattatatct tttaaaacat catttgatag aaatttaggg taagtttcag
     9781 aaagttgttt tacaatatta ccttttaaat ctgaaataat taccttacat atatttcttc
     9841 ttattaggat tccacaaata caaggcgata tattagaaat atctaataaa ataggtttac
     9901 gacctgatga atgtgcattt aaagttgatg actctatttc cctaatcata ttttcattaa
     9961 taagttcagt aactagattt gataaagtca ttttagtaag tcctgtagac ttagctaaat
    10021 caactctgga cataggtgta tttgtagaaa gtaatttcaa tattattaac ttatttttaa
    10081 tttttacatc tttgatgtta gaaccttgtt tgttactcat cataaatctc cttaaataaa
    10141 gttaagtgtt ttatttattt tcaagtatac tatcacctta taatagagta acattttttt
    10201 acttgaaaat atcattaatt agaatatatc acattatata tcataaaaca tataaaaaaa
    10261 tattagaatt aaattaaaag tgaaaagtag attgatatat aaatagtaaa tacttaatta
    10321 aaaaaagata atgatagcaa taattattgt taccaagaat ctactacttc aagtgttacc
    10381 ttaggtattt aagttgatag tggtttgcac gtaataaact tgatgtaata tatattctta
    10441 ataatatcga taaaagattt acttaaaaat aatatagatt atgtaaacat ataatctatt
    10501 tattgtatac aataaaattt cattttgtaa aacagtgaat taaatatgta atatagatat
    10561 cgatttttat tacaaattgt tactaaatat aggtacatta gaacaaaaat ctagtaaatt
    10621 gtaactaaaa tgtaaat
//
LOCUS       DAYAOX010000129        10613 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.129, whole genome shotgun sequence.
ACCESSION   DAYAOX010000129 DAYAOX010000000
VERSION     DAYAOX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.129"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            83..1063
                     /locus_tag="ACGI57_002531"
     CDS             83..1063
                     /locus_tag="ACGI57_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFG9717601.1"
                     /translation="MNLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLL
                     LYYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVL
                     KLLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIV
                     PTSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKL
                     GVIILGMCWSLWHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCA
                     IIHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1162..2124)
                     /locus_tag="ACGI57_002532"
     CDS             complement(1162..2124)
                     /locus_tag="ACGI57_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFG9717602.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(2440..3390)
                     /gene="hpdA"
                     /locus_tag="ACGI57_002533"
     CDS             complement(2440..3390)
                     /gene="hpdA"
                     /locus_tag="ACGI57_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HFG9717603.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3383..3640)
                     /gene="hpdC"
                     /locus_tag="ACGI57_002534"
     CDS             complement(3383..3640)
                     /gene="hpdC"
                     /locus_tag="ACGI57_002534"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HFG9717604.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3644..6352)
                     /gene="hpdB"
                     /locus_tag="ACGI57_002535"
     CDS             complement(3644..6352)
                     /gene="hpdB"
                     /locus_tag="ACGI57_002535"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HFG9717605.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(6649..7665)
                     /gene="tsaD"
                     /locus_tag="ACGI57_002536"
     CDS             complement(6649..7665)
                     /gene="tsaD"
                     /locus_tag="ACGI57_002536"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFG9717606.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(7671..8147)
                     /gene="rimI"
                     /locus_tag="ACGI57_002537"
     CDS             complement(7671..8147)
                     /gene="rimI"
                     /locus_tag="ACGI57_002537"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFG9717607.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(8137..8853)
                     /gene="tsaB"
                     /locus_tag="ACGI57_002538"
     CDS             complement(8137..8853)
                     /gene="tsaB"
                     /locus_tag="ACGI57_002538"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFG9717608.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(8853..9305)
                     /gene="tsaE"
                     /locus_tag="ACGI57_002539"
     CDS             complement(8853..9305)
                     /gene="tsaE"
                     /locus_tag="ACGI57_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFG9717609.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(9444..10484)
                     /locus_tag="ACGI57_002540"
     CDS             complement(9444..10484)
                     /locus_tag="ACGI57_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFG9717610.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
BASE COUNT         3417 a         1914 c         1093 g         4189 t
ORIGIN      
        1 tattgactgt cattaattta taatattcaa taaaatattt taatttagtt aagaatctat
       61 attgtaaagg agtattaaat taatgaactt aaaattaata tggataaata ttctaaaccc
      121 agtatttatt aaaaatgaaa taaaaagaaa aaaaatacaa attattatct ttttctttat
      181 aaactttggt ttaacttatt cattaggttt actgttatat tataataaat ttattgatcc
      241 agaaaacttt gcatcattca tgatgatatt acctctatct tcagttgcca ttgcaaagtt
      301 ttatactgaa ggattaaata atgatatgta tgaattttat tcactaatca tattattttt
      361 tcttatttat tcattattaa ttgttcttaa gttattaaat ctaataaata ataatgaatt
      421 tataattata aacctaatat taacttttat aagtagctta tcaattataa tatattcatc
      481 aaataaaaaa aatttatatc ctgtaaaaaa tattaaacta ggttctttat taattattta
      541 ctttatgatt tctgtaattg ttccaacatc atttatacta atactacaag gggctcaacc
      601 taattatagt ggtatcataa aatttatatt tactccaata acatcattaa ctactattta
      661 tatttttttt agtgaagaat atgcctggag aggtttttta cagaatattt tttttgataa
      721 atttggtaaa aagttaggag ttataatatt aggtatgtgt tggagcttgt ggcacttacc
      781 actaatattt acattatata cacctgaagc gccaatatta ggaataatat tacgttcaat
      841 acatatagtt ggaatttcaa tatttttagg atatctttat ataaaaacta agaatatctg
      901 gttttgtgct ataattcatg ttttaaataa tagtgctttt cttatcacaa attctaaaga
      961 atcaactatc acttaccatg atatactaat agtatctatg gttgttttaa ttttttatgt
     1021 tccatttcta tttacaaaag aatataaaaa taacttggag taaatagaat atccaagtta
     1081 tttttatatt tttgcaaatt tccaacaatc ctacagaata ctttaagctt agaagatatt
     1141 attaaaattt attataatca actaattctt gatattggtc acatttactt tatcactaac
     1201 aaaatcatac ttagaatctc tatagtaata ccatacaaat ataaagctaa gtagattaac
     1261 aaaagtagtc aaaattccac cttcaatacc aaacattcct ccatttaata gattaccatg
     1321 agtgatttgt gtagttataa tccctggaac ttgaagtcca cttacaggca taccatatat
     1381 aatcccctga aatacattcc atgcaaaatg ataacctata cacatccata tattcgacga
     1441 ttttataaac atatatgaaa aaagaatccc aataattata atattaatta cagacaaaaa
     1501 agtaacatca ggattccata tatgaatcaa cccaaaaata acagatggaa caatcattat
     1561 aaaataaata tttttacatc ttcgtaaaac tgccataaaa aaaccacgat ttaaaatttc
     1621 ttctgcaaaa gcaactaaaa taaagaccag tatccaagaa actgttaaag gtgaaatttt
     1681 tggtacccat gaagtaacat gaccaccacc aattgttatt gttataataa agattactgt
     1741 acaattaaat attcctaata acattcctac atatccttct tttttcttaa ttctataaga
     1801 atgtaatccc atttcactaa taggattttt cataataaat ctccatacaa ttataggaat
     1861 aataatcata agtgaatctg ttagaatttg aaatacaatc tgtaacccaa catcatttaa
     1921 ccaattagca tattctgaaa aatagtttgt ttttaaatta atgtcacctg tagcaaccag
     1981 gatttttctt attatttcca caagtatcat gcttaaataa tactgaagta tgtaaaaggt
     2041 agccataaca gctataataa tccaccctga acgtactttt ttgtctttat tcttaataat
     2101 caagttgctc catgatttgt tcatatagat gtcttcctcc taactattat ttttagttgt
     2161 ttctaaaata tattctatta aattttttca tagtagtatt ttaccacaaa acttctgctt
     2221 caatgaagta agcgtaatat attctctaac tgtaatttaa actataaaaa atatataaag
     2281 tatatttaaa gcaatattta tggacataaa gcaatataaa caatatataa aacatataaa
     2341 actttactta attatttcta cataaaaacc acataaaaaa ggatgtaact ctagctttta
     2401 gtgtaacatc ctttttttaa ttaaaacttg tatttaattt tagaaagctg tctcatgacc
     2461 aacataacaa gctataccat tatccaaaaa tatatcttgt aattcttcaa gatgaccttc
     2521 atcaacatca cccttatcag aatactcata ttccttgcct aattgaatcc attttgattc
     2581 tcctaatctg tgaaatggta atagattaat ttctaccaaa ttatttttgt gcataaaaga
     2641 tataatatca cttatatttt caacactatc attaaaccca gagataactg gtactctaag
     2701 tactaatctt ccattccaat cagaatttgc aaggtttgat atattttcta atattaaatc
     2761 attatacaca cctgtttgtt ctttgtgttt ttctctatcc atatgcttta tatcaataaa
     2821 tgcaaaatca atatcgttaa atattttatt aaaaacttca tttgatacac atgcactcgt
     2881 ttcaatggca gtgtgtatat ttacctcatg acactttaat aatacttcat gtaaaaattc
     2941 atgctgaagt agtggctcac ctccactaaa tgttactcct ccattacttc tccaattgtt
     3001 agaatcacgt ttaattacct gcacaagttc atctacagtg taaggttttg cacataattt
     3061 aaacgcatta taataacatg agttgacgca ttcaaaactt tcacagtcct tacagatgtt
     3121 ccaatctata actggcttat tatcaagatt aaagctcaat gcaccatttt tacacttacc
     3181 atgacataca gtacatccat tttcatattg acaagataat tcactaaaca tcatatgtgg
     3241 tcttacagtc caactttctg gatttgcaca ccatttacaa ctcagtggac atccatttaa
     3301 aaatacagtt gttctacaac ctggaccatc atgaactgaa aagctttgta catcaaaaat
     3361 catgccttct aattgctttt gactactcat taaacacatg tccttcacaa gtaattgcat
     3421 tcaatgttga atatacccag tcttctttct ctaggccaac acatttgcct ataccttcat
     3481 cattagcttc aacaaaattt ttacaatttt tacattttgg cattactttt atctctggac
     3541 atgctgcatc atctaaatta atcatttgtt ttgataatct acaaattcct ttggttgcat
     3601 ctactgcaca aaaattcata caatcactat gctttctcat attttacacc ccttcatact
     3661 ctgttctagc aattacttca tcttgtattg gtttacctaa ttcacaccaa tattgagtaa
     3721 acccagcaac acgtaccatt aattgacgat aattgtcagg atttttttgt gcatctttta
     3781 atgtttctga atctacaaca ttatattgaa tatggaaccc accttttctt aaataacttc
     3841 ttgttaaatc taataatttt ttagttcctt gttgtccttt tatagtagtt ggatgaagtt
     3901 ttaaattcat ctgagaattt tgaactactg cttggtccca aatagttgca gattcaaata
     3961 atgcatatac tccattttta tctgttcctg catatgcaga gactgaacca tctgaatatg
     4021 ttgttccact tagtctacca tctggagtag ccaaggtagc agctccctgt ggcccatgtg
     4081 tagaaacaga tatttggcaa gggtacatct tcttagcata taaagattgt gcttcttcac
     4141 atacttttga taaccatacc tcatagtttt taagaatatt atctgcatac aaatcattat
     4201 ttccatattt aggtgcatca agacaggcct tataaatagc atcatattta ccagtcttat
     4261 ctacttttac ttggtctgcc atagaataat ttcctacttc taatgcatct ttaaatccaa
     4321 aattatttaa tatggcatcc ttcatttcct ctattgtaaa tgctttgtca tcatatacaa
     4381 gtttcttaag tgctgcaaat gaattgacca tagtaacagt accacaagtt tctacgttta
     4441 atgttgcatt ataacgatat cccatatttc ctatatgttg acctttgtct aaacagtctg
     4501 gctttagtag tgaatttatt atagaagtat caaattttct ccatatatct aactcaatgt
     4561 tatttgctct ttctaaaaca tttattgctt gtttataata atctttaaat acttctatga
     4621 cttcttcata agtatctaat ggcttattat gaggctcaaa tacttgtata ttcatacgat
     4681 agtctttacc attcataaga acaagttcta aaatttttgg attagctata aagtgaactc
     4741 cagagccagc agattgacct gctccacctg caatagagta tgtttttccg tttaaagtta
     4801 actgtttcca acaccctgga gacgtttcca aacatccacc taatgcaaat gctcttgctt
     4861 cttcaacagt cataccttca tcagcaaatt gtttcatcat aaatgttgtt ccatttgagt
     4921 tgtttaccca tgctggataa ccagaaccaa gctttgtaca ttcagcagct ttcattaaga
     4981 aatcttctgg aagcttttca tcatatagca tagttaaact aggttgagga gttctacatc
     5041 tcatacttgc ttctaataat aactcttcta gttcattagc tcctgtagaa ccatcttctt
     5101 ttaatccacc tatagataaa gtattaaatg tattgcctga tagtactcct ccatttacgc
     5161 ccgctgaagc aaaacagtca atacaagtga tttttattct ataaagctct aataattcga
     5221 taacctcttc ttttgtaatt agtccttttt ctatatcttg ttcataccaa ggatataaaa
     5281 tttgaccaaa acgtcctata gacataccag atatagcatc ctcatttact gatgctatat
     5341 gaattgtata agtcaactga agagcttccc taaatgtacg aggttgttta tgtgcaatat
     5401 gttctaatat ctccactaaa tctgagtatt cttttttagc ttctaaatct gtctctatac
     5461 tttctaaata ttttgcatgt tttgcataat ttaaaatcca agtttgtaat ccttcaataa
     5521 cttgaataac agcctcataa aaatatagac ggtccatacc aataagccca tctccagatg
     5581 catttcctgc aactgccttt ttacattctt ttgccatttc tataattcca tctaatccat
     5641 aatttaaagg ataaaaataa tttataactt ctctaccttg aggaagtgta taccctgaat
     5701 caaacatgca tataagtgta ctcattaggt tttcttttaa atcataatct ggcatcattt
     5761 tttcatagtg aaaacctaaa tcttcaacag actttccaac ccattccttt gccattttta
     5821 caagtacagg tacttcttct tttctcattc cgaatttacc agctattgaa acgacatttc
     5881 caaaactttc agtaacattt cctccaccag acccaaattt tgtaagttca tcagctgtgt
     5941 tactggctaa tttctttgcc tcttctctca tttgttctcc ttgagcaaca aagaagcttt
     6001 cacttaccca tggcattgga aaagaacctc tataatggcg tgtcttttgc attactattt
     6061 tctcaccagg tattatattt ggtgtcatat gtgaaaaagc agctttaaga gctttagctc
     6121 ttctaactac tggtatatca ccatctgatt ttctatattc tctattatac caatatggaa
     6181 attccatatc agcagtagat aatgtattat aatatacatc taataagtct tctgctcttt
     6241 tacttgcttt tttttcatta aattcactta aattttcttt attttgaaaa tttgatttaa
     6301 tattttttgc ttccaaaata cttcttattt tgtcttcttt actttgactc atttcttccc
     6361 ctccttaatc tttctataat tagtataaaa ctttttaaga atgaaaaaaa agggtatatt
     6421 acccactttt taaaataatc tttctataga aaaaaataaa cttgaagcaa atctaagcca
     6481 aagacaatct gtatatccta aaatttgttg caattcattg gaatataaag attataaata
     6541 gttttacttc aagttttaat atatactata tttattttta ttatatttaa tttcacttct
     6601 acttactttt ttgaaacaat acttttatat ttacaaattc atctaactct actgatttat
     6661 ctttaaattt ggaactgcat ttaaactcat atcatgcgtt ttcccattta taaaattata
     6721 atatcctgca caccctatca tagctgcatt atctgtgcat aaaattagag gaggatactt
     6781 aacagtaata ccattgtctt tagcaagctc tgttatttta gctctaagcc ccgaattaga
     6841 agcaacccca ccagaaagag ttatgatatt gtatcctttg tcttttactg cttttagtgc
     6901 tttagtagat aaaacctcta ctacagcctc ttggaaagat gctgctacat cttcaactac
     6961 tatttcttca tttttcattc tttttccatt tagataattt aatacagaag attttaatcc
     7021 actaaaacta aaatcatagc tatcttcttc taaataggct cttggaaatt ctatagcatg
     7081 tttatttcca ttcttggcca gattatcaat aataggacct cctggatagc ctaaattcat
     7141 agctcttgaa atcttatcaa atgcttctcc agatgcatca tctcttgttt ttcctaaaat
     7201 ttcatacttg ccataatctt ttacttcaac caagtgagta tgacctccag atactattaa
     7261 tgttataaaa ggaggtttta aatccttatg ctctatatag tttgcactta gatgcccttc
     7321 tatatgattt actcctacaa gtggaatatt caaagtatat gcaagtgctt tagcataaga
     7381 tagaccaacc aataaagcac ctactagccc aggtccatat gtaactgcga tatggtctat
     7441 atcattaaaa cctatatttg ctttgtctaa agcttcttga acaactatat caatattttc
     7501 cacatgtttt cttgaggcca cttctggaac tacaccacca aattttttat gtgtctcgat
     7561 ttgagtagag attatgtttg aaagtacttc tctaccattt tttaaaactg atgcagctgt
     7621 ttcgtcacaa ctactctcta tagctaatgt tattatatca ctcataaact ctacacctct
     7681 tttaattggt tccacattat aattgcatct tctttattat cactgtagta ttcttttctt
     7741 attccaccca ttttaaatcc atattttcta tataaatttt gtgctatttt atttgatgct
     7801 ctaacttcta atgtcataga tgcaatatta ttatctttac ataaatcaac tatgcctttt
     7861 atcaacttgt cccctatttt tttccctcta tagtcactat gaacagccac attatttata
     7921 tgcccttcat caagtataag ccaaattccc acatatccga caatttttcc atttaactta
     7981 gctactatat accttgctac ttcattagac aactcttttc taaaagactc ttttgaccaa
     8041 taatcttcaa aacaattttt ttctacttca aaaacttcat ctatatcttc tgtagtcatt
     8101 tcttctattt ttatatcatc tactattagt tttgtattat cttccattat ttagcctctt
     8161 cattttttca tcatattgta cttcagcttg agattttctg atatacatag gatttatcgt
     8221 ataacaagta tgtacatcta tatttttatt atatttattt aatgctaaag agcataagct
     8281 tcctgcgtta gatacattat tagctggtga aggtattttt atatttttta tattctctaa
     8341 cttgtcttta tatttgtata cagcttctcc aactattata aattcttcat tagttgatac
     8401 aatttcatct actaaagcat caaactctac aacatcaaca ccatctaatt ctatcatctc
     8461 tccattttca aacttgtatt tagctgaata aacttgattt ttctgtgcat ctaatataca
     8521 acatatcttt ctatcacaga aatttatgtt atttgctaga gattccaaag aatttactgc
     8581 tattattggt atattattta catgggccat agcttttact gtagccatac ctattctaag
     8641 tcctgtaaac gaacctggac ctatacatac agctaataaa tccatatctt ttatacttaa
     8701 atcactcata ctcaacatat tttctatcat tggcataagc ttttgtgaat gagttttttt
     8761 gttgtttact gtaaactcac atattaagtt atcatcttca acaactgcta cacttgctgc
     8821 catagaagac gtatccatac ctagtatttt cattatttaa tcaactcctc aactatctct
     8881 ttataaaact ctccttttgg agttaatatc atttctcttc cttcatcttt atatctcaat
     8941 tctatattca aatattcttt tggaagtata tcttctatta gattagccca ttctattata
     9001 cagattccat ttgaatttac atattcatca taaccaatat catacatttc atcactacta
     9061 cctattctat aaacatcaaa atgatacaaa ggtatttttc cttcatattc atttattata
     9121 gtaaaagtag gacttgttat ataatcttct atccctaatg aatctgccaa actttgtgtc
     9181 attgtagttt taccagctcc taaatctcct actaaacaga taacactgcc ttcttttaaa
     9241 agtttaccta atttatatcc tatttctcta gttttatttt cattttctaa atatattttt
     9301 gccatcaatt catctccatt ttgatataat cgtcatgtga atttatatca ctgcttaatt
     9361 aattatagta taagcctaga ttattataaa gtacttgtct ttatcttttt ttatctttca
     9421 ctattgatgg ctgtatctac accttatata caatcatcaa tagcaatttt tacatactct
     9481 tttgctttat ggttttgctt caatgcttct ttacatattc tctcagattg ctcttttaca
     9541 tacttaagag acttatagtt ctcttttact gcttcaatac acattttttc tgtttgattt
     9601 tttatataca taagcatttt tccattttgt cttacaatct ctatacatat atcttctgta
     9661 tacttatcta tgtaacttag tacatcttta atattatatt ttttatttaa atatcttata
     9721 gcatttatac atatttcttc tgtcttattg ttgacaaatt gtatacttct tccatcttgt
     9781 ttgacagctt ctatacatat tttatcagtt tgtttattta catattgtaa tgccatacca
     9841 ttttgtctta cggattctat acacattttt tccgtttgat tttttacata ttttaatgct
     9901 ttataatttt gcttaatggc ctctatacat aattcttcac tctgttctct aacgtatgca
     9961 agacacctgc catctttttt aattgcttct aaacatatcc ttcttgtttg agtctctaaa
    10021 gcctttagta tacttacaac atctatattg gaagaatgac ttattgcttt tataataagc
    10081 ccttctgttt tatttcgtac atacttaagt gcattacaat ctttttttat agcttcaaga
    10141 cacaagtatt ctgtttgttc ttttacatat tcaagtgctt tataattata ctttattgct
    10201 tctaaacata attcttcggt ttgatttttt acatactcta atgccttgta attctgcctt
    10261 attgctaaaa tacacatacc atcagtttga ttttctatat tttttaattc acgataatcg
    10321 tattttaccc tttccatatt ttcaatatat tcattagtga tatactttaa agaatctcct
    10381 aattttgtat cataaatacc aatatgaata tctttccaat tactattaaa ataataccct
    10441 ttatcatcta aaaataattt ttttattttt ttagataaat gcatgaatat acacccctat
    10501 cattattaaa ttagttatta ttaaaagttt gattattaat ctttatagtt ttttaataat
    10561 aactttattt tttgtttatt ataaattcgc tctgtatatt gtagatacct ttt
//
LOCUS       DAYAOX010000130        10276 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.130, whole genome shotgun sequence.
ACCESSION   DAYAOX010000130 DAYAOX010000000
VERSION     DAYAOX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.130"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            266..1183
                     /gene="pstC"
                     /locus_tag="ACGI57_002541"
     CDS             266..1183
                     /gene="pstC"
                     /locus_tag="ACGI57_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFG9717611.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            1185..2009
                     /gene="pstA"
                     /locus_tag="ACGI57_002542"
     CDS             1185..2009
                     /gene="pstA"
                     /locus_tag="ACGI57_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFG9717612.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            2028..2792
                     /gene="pstB"
                     /locus_tag="ACGI57_002543"
     CDS             2028..2792
                     /gene="pstB"
                     /locus_tag="ACGI57_002543"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFG9717613.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            2820..3473
                     /gene="phoU"
                     /locus_tag="ACGI57_002544"
     CDS             2820..3473
                     /gene="phoU"
                     /locus_tag="ACGI57_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFG9717614.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            3631..4500
                     /locus_tag="ACGI57_002545"
     CDS             3631..4500
                     /locus_tag="ACGI57_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFG9717615.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            4587..6023
                     /locus_tag="ACGI57_002546"
     CDS             4587..6023
                     /locus_tag="ACGI57_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HFG9717616.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     NLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            6071..6898
                     /locus_tag="ACGI57_002547"
     CDS             6071..6898
                     /locus_tag="ACGI57_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9717617.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    7194..7456
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7532..10198
                     /locus_tag="ACGI57_002548"
     CDS             7532..10198
                     /locus_tag="ACGI57_002548"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFG9717618.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         4128 a         1090 c         1955 g         3103 t
ORIGIN      
        1 ataaactgta ttattatcta tatctgctat tttaatatag agaaacaaca gtatattgta
       61 aagtaaatgt caaatatagt tttaaaagta aaattaatgt taaaaagtaa atataatttt
      121 acacttgctt aacttaatcc taatatagat ttaacttaaa aaaagtagaa taaaagtaca
      181 ggaaatttga tgaagacgaa ttataaaaaa ttaaattttg aatacagaac gaaaatcgat
      241 aagtatattt taatgggagg gtattatgat agtaaaatct agattagtag gtttagaaaa
      301 aaatgacaat aatggcaata agacaaaata cgtaattgaa aaagtagcaa aaaatatttt
      361 tctacttagt gctttagtag cagttgcaag tttacttttg ataataggat ttgtatttta
      421 taaaggatta agaccattta tatttgaagg atattcattt atagactttt taactggtgc
      481 agattgggtt ccttctgcca ataaatttgg aatatcatct atgatagtag catcaatatt
      541 tgcaacactt ggagcacttg ttataggagt acctgttggt atattaacct ctgtatttat
      601 agcagaagta gcacctaaaa gattagcaaa aataatgtca ccagcagttg aacttttagc
      661 aggtatacct tcagtattat atggtgtatt tggtcttgca gtgatagttc caactataca
      721 aagtatattt aatcttccaa aaggtcaaag tttgttagca atagtaatag tattagcagt
      781 aatgatgtta ccaacaataa tatctgtatc agaaacagca ataagagcag taccaaaagc
      841 atacaaagaa ggttcattag cacttggtgc atcaaaaata gaaacaatat tcaaagttgt
      901 attacctgct gcaaaatctg gagtattagc cgcagtagtg ttaggagtag gaagagcttt
      961 aggtgaaaca atggcagtaa tacttgttgc aggcaactca ccagttatgc caactgcaat
     1021 aacagatagt gtaagaccac ttacaactaa tatagcactt gaaatgggat acgcatttgg
     1081 tacacatcaa gaaatgctat ttgcaactgg agtagtattg tttacattta tattaatttt
     1141 aaacttagtt ttaaataaac tttcaaataa ggcggtaaat taagatgaga aagttcaaag
     1201 agaatctatt gaagtcatta gtatatctat cagcactatt tacaatagta tcattagtag
     1261 ttatagtggg atttatattt ataaagggaa ttggcaacat gaattttaat ttcttattta
     1321 gcaattattc agcatcaggt gatggtggta tacttccaat gataattaca acattatata
     1381 cagtagtagt ttctatagtt gtagcaactc ctataggaat attggctgct atatatttac
     1441 aagaatatgc taaaaaagga aaagcagtta cagctataag atttgcaact gaaagtttat
     1501 cagggatacc atctatagta tatggattat ttggaggaat tttctttgta gttacattaa
     1561 aaatgggata ttccatagtt gcaggttcac ttacagttgc aataataata ttaccagtaa
     1621 taattcgtac aacagaagaa gctttaaaaa cagttcctca agcttataga gaatcatcat
     1681 tggcattagg agctacaaaa tttcaaactt tatataaggt agtacttcca agtgcaatac
     1741 caggaatttt atctggagta atcttatcag ttggtcgtat aattggagaa tcagctgcaa
     1801 tattacttac agcaggtact gtagccaaga tgccaggtgg aatatttgat agtgcaagaa
     1861 cattaactgt acactcatac ttactgacta aagagagtgg agatatagca acagcagcca
     1921 gtataggaat tgttttaata gtaattgtat tagcactaaa catgctagca agatttgttg
     1981 ctaagaaatt aaataaagct aattattaaa tttaagaggt gtgaataatg gaattaatcg
     2041 ataagataaa aatgagtgta aaagatttgg atttatttta tggtgataag caagcattaa
     2101 agaaaataaa tatggatata aaagagaata aggttactgc tttaataggt ccatctggat
     2161 gcggaaaatc aacttttata agaacgttaa atagaatgaa tgacttaatc gaagatgtaa
     2221 caattaaagg aaacatttct gttgatggag aggacatata tacctcagat gatgttataa
     2281 atcttcgtac taaagtagga atggtatttc aaaagccaaa tccatttcct atgagtatat
     2341 atgataatgt ggcatatggt cctcgtacac atggactaag agataagaag caattagata
     2401 aaattgtaga agaaagctta aaaggtgcag ctatttggga tgaggttaaa gacaggctta
     2461 aatcatctgc actaggactt tctggtggtc aacaacaacg tatatgtata gcaagagcaa
     2521 ttgctatgag accagaagtt attcttatgg atgaacctac atctgcttta gacccaattt
     2581 caacattaaa agtagaggaa cttatagagg atttaaaaaa agactacaca atagtcatag
     2641 ttacacataa tatgcaacaa gctgctcgta tatcagatga aacagctttt ttcttaaatg
     2701 gagaggttat agaatttagc gatacaaaaa ctatgtttac cactcctgta gataaaagaa
     2761 cagaagacta tataacaggt agatttggtt aaaatatata actataaagg agttagacaa
     2821 tggtcaataa tagcttagat ataagtattg atactttaaa agaacaaaca gtgcttatga
     2881 tggaaaaatg tgaagaagca atagaaaagt ctgtgacttg tatgatagag aaggatttag
     2941 agggtgcaag aaaagtagta gaagatgatg aagaaataaa taaattaaga gaagaaataa
     3001 gagataagag tatagagcta atagtattaa agcaaccaat ggcaagggac ttaagattta
     3061 tttattcatt aggtaatata tctatagagc tggaaagaat aggagattat gcagcaaata
     3121 tagctagaga aatgttgaag ataggtggag aggcttatat aaaagagcta atagatattc
     3181 ctaagatggc aaaagaatgt aagtctatga tgtctcagac tagagaagct ttaatcttat
     3241 ttgatgcaag tatagcttat gatgttggaa ctaaagatga tattgtagat ggattgtatg
     3301 aacaagtaca aattgattgt ttaaaaataa tgcatgaaaa ctcaaatacc atagaccaag
     3361 gggtaaaact tctacttata ggaagatacc ttgaaagaat tggagaccat ataacaaatg
     3421 tatgtgagaa gataattttt gcaattgagg gtaaaatggt tgagatagga taaaaaactg
     3481 ctttaaagca gtttttttat tgtctaaatt aagaaagaat tgataaaaaa atatgattaa
     3541 tgggtaaatt taaaaaattg tgatatacta ggaacatatt aaatatgagg atgcgtattt
     3601 tatataaggt gctttttagg aggtggaact ttgaaacttc aaaagatggg tatgaggact
     3661 ataaaaacag gaattagtgt cactttatgt atgattcttg gtgactattt agtagagaat
     3721 atgttttatt ctctggtagc atgtataatt tctgttcaag atacagtaaa aggttcttta
     3781 aagacgggaa tcaatagagt ggaaggtact attttaggtg gtataatagg attcatattt
     3841 gccatcatta aagctggaga cccattttta tgtggtctag gagtaatgtg ctcaatatat
     3901 ttatgcaatc tgtttaaaat aaagtcagta gtagttgcct gtgtaacctt tctggctata
     3961 catctaggtg taggaaatag tgaccctgtg tattattctt tacatagggt gttagataca
     4021 tctgtgggtg ttttgtttgg agtacttgtt aattattatg ttgctagacc aaattatcta
     4081 aatagtgtta tagatgagtt taaaaagata gaagaacttt caatgttgct tattgaagat
     4141 aaaattctta gaaaagaaaa tttaaatgta gagaaatttg aagaagaagt agaaaaatta
     4201 gaagaaatat attcaaaact tattgatgag ttggattata gtttgagtga aactaatata
     4261 gaaaaaattg agaatgcctt aaggatatgt agagaaatac attttcatat gcagtctata
     4321 gagttacttg aaaataaact ttatttgaaa gagcatacat ataaatacct taagaaaaca
     4381 tataatgtgg ataagctaga ctggaatata gatgaagaaa aaagccctgt attcaactat
     4441 catctaatga aaataatgat tgaaatagag ttgttgagaa aactaaataa atcatattaa
     4501 taatagtagt taaataataa ataaaaaata gtatctttgt aaatattaaa catatataag
     4561 tttaatttat ggaggtacta ttttttatga atactagaaa tgacaattct aaaatactag
     4621 aaatatataa aatgaagctt tttggcgaac ttgtaaaagc agtgtggaat gatgacatag
     4681 aggatattga aaacttacct aataaaattt taaatgaaaa tttaaaggat aatttaaata
     4741 attattttag tggaaaattt gataaagata ctatggtaaa tttaattagg gtaactatgg
     4801 gattggatgt gacagaagat acagataaaa atttaaaaga gtcactacat gaagctgtaa
     4861 atttagatga agtcaaaaga ccaataatat ctgtaatatc agatgcttgc aaatattgtg
     4921 atgaaaataa taaggaagat gagtcatgta agataagaag taagcatatt aattgtaatg
     4981 aggaagatgt atgtagtgct tgtggtgatt gtgtatcaag atgtaagttg ggtgcaattt
     5041 cagataaaat agaatttata ccaatgataa aattgttaaa agatataaag agccctgtat
     5101 atgcaatagt tgcaccagct tttgtaggac agtttgggaa agatgtaagc cctggtaaat
     5161 taagaactgc attaaaaaag ataggatttg aagatatgat agaagttgct ttggcagcag
     5221 atatgcttac tgcaaaagaa gcatatgatt attatgagca tatgaaaaag gacgaagaag
     5281 gatgctttat aactagttgt tgctgtccta tttgggttag tttgatacaa aatagttttc
     5341 ctcaaatatt ggagaatgta tcaccatccg tttctcctat gattgcttgt gggagagctg
     5401 taaaaatttt aaatccagat gctaaagttg tattcatagg tccttgtatg gcaaaaaaga
     5461 aagaggcaat gttagaagat ataaaagatg cagttgattt tgtgcttaca tttaaagagt
     5521 tgcaagaaat atttgatgca ttagagttaa atccagcaat aatggatgag agtaatagag
     5581 tagaggcgtc cctttcaggt agagtttatg ctagaacagg aggtgtaagt aaagcagtta
     5641 agttatcagt tagaagaatt gacaaaaata taaaatttga agaaaaggca tttgaaggaa
     5701 caaaagagtg catggacggt ctaaagaaag ttctaaataa agaaatagaa gctacattta
     5761 tagaaggaat ggggtgtgta ggaggctgtg taggaggtcc gaagcgtatt ttaagtgtgg
     5821 aagaaggtac taagaatgtt gatgaatata gtgaaagtac gaatatggaa actccatttg
     5881 ataatttaaa tgtattacaa tttttaactt ctttgggaat caaaagagta gaatctttag
     5941 gagaaaaaga agaagaaaag gttaaaaaaa tatttagtag agatataaaa agtaataaga
     6001 aagagaataa atataaaaaa taacttttta agtcttaaac ttatgctttt ataaaaggga
     6061 gatatggggg atgaaaatta aagataataa ggctgttttt catgtattta taaggatatt
     6121 tcttatagct gttttaattt taactggaac attattggag gaaaaaatta ataatgttcc
     6181 agttgaaaat ataagttcaa aaggtgaaaa tacactggaa ctgctttctc aaagtaatac
     6241 tttaggagaa atcatataca aagcatctca agaagaaaca aaaggtccag gggctacttc
     6301 tgaaaaagta gcatacataa caatagatga tggtccatca aaatatacaa atgcactact
     6361 tgatatttta aaagaaaatg atgtcaaagc gacatttttc atgcttaatc agaatatgaa
     6421 aaaccacaag gaagaagtaa ggagagtctt ggaagaagaa aactcaatag gttttcatag
     6481 tgtaagtcat gatatacata agttatataa aagcccagaa gtaacagttg gggaatttaa
     6541 tacatgcaat aatactctac aagaaataac tggtcagagt tcaaaattaa taagattacc
     6601 atacggaagt aaaccatata tgccagaagg ttcatataat aaacttatgg caaatgatta
     6661 tttaatttgg gactggaatt tggatacaca ggattggaag tcttctacaa gtcagatagt
     6721 aagtaatata ttatattatg gaagaaaaag agatgaatta gtagtattaa ttcatgagaa
     6781 agagcaaagt ttaaatgctt taaataatat tataagaatc ttaaaagaga gaggatatac
     6841 aatattgcca attacagaaa atataacacc taaaaatttc tggcaagaaa acttataata
     6901 aatttaaata tagtaaaaga taaagtaaat tatttataaa ataaaacata taatataaaa
     6961 aatacgaata agaggtatac tgtaaggata atataaaatt aatgagaaag cattaaaatt
     7021 aaaataaaat aatcttgcaa ttattgacat tataagacag aataactgta aaaacatatt
     7081 ttaaaatata acaactatat aaaagagaat agacataatt aagaaaattc agaaaaaata
     7141 aaataaaaat aattgacaaa gagaataata tttgtatata ataatatgta atattaaaga
     7201 ctgagaagaa gaggagtaag taaatactac taaaagagag gaaaattcac tggctgtaag
     7261 attttctaag tataagattt actgaatgta gctttggagt ttctaaactg aactataagt
     7321 aagtttagac ggttagaacc gttaaattct ttgagagtca aagttgataa aataacatta
     7381 accaataatt taatgatatg ttaatgtatt ttaagctttg gaattaaggt ggtaacgcga
     7441 gcttttcgtc ctttttaaag aggatgaaga gctctttttt atttctaaag atatttaaaa
     7501 aatttattta taaaatacag gaggcaggac aatggaaaat acgaatttat caaaaactta
     7561 taacccaaaa gattttgagg ctaggctata taaaaaatgg atggatgaag ggtattttaa
     7621 atctaaacca aatccagata aaaaaccatt tacaataatg atgccaccac caaatataac
     7681 agggcaattg catatgggac acgctcttga ccatacactt caagatatat taatcagatg
     7741 gaaacgtatg gatggatatg aagctttttg gcttccagga actgaccatg cttctatagc
     7801 aacagaagtt aaagttgttg aaagaataaa aaaacaagaa ggcaagacaa agtatgaaat
     7861 aggtagagaa gaatttttaa agagagcctg ggaatggaaa gatgaatttg gaggaaaaat
     7921 atctaatcag cttaaacaac taggtgattc ttgtgattgg gataaagaaa gatttactat
     7981 ggatgaaggg tgtaatgaag cagttataga attttttgta agcctatatg aaaaaggtca
     8041 catatacaga ggaaatagaa taataaactg gtgcccagat tgcaaaacta ctttatcaga
     8101 tgcagaagtt gaacatgaag aacacgacgg aaacttttat catataaagt acccactaaa
     8161 agatagtgaa gactttttag aaatagctac aactagacct gagacaatga taggagatac
     8221 tggtatagca gtaaatccag aagatgatag atataaacat ttaataggaa aaacagcaat
     8281 acttccatta gtaggaagag aacttccaat agttgcagat agttatgttg atttagagtt
     8341 tggaactggt gcagttaaga tgactccagc acatgaccct aatgattttg aagtaggatt
     8401 aagacataat cttgagcagt taaatactat gaatgaagat ggaactatga atgaagtttg
     8461 tggaaaatat gaaggtatgg atagatttga gtgtagaaaa gctatcgtag cagacttaaa
     8521 agaacaaggt tacctaatta agataaaaga gcataatcat aatgtaggaa cttgttatag
     8581 atgccataca gtagttgagc ctagattatc agagcaatgg tttgttaaga tggaagaact
     8641 tgcaaaacca gctatagata ttcttaaaaa aggagaatta gaatttgttc cagataaatt
     8701 tgataaaact tatttacaat ggctagaaaa tataagagat tggtgtatat caagacaatt
     8761 atggtggggt catcaaatac cagcttatta ttgtcaagag tgtggagaaa tagtagttgc
     8821 tagaaaaatg ccagataagt gtcctaaatg tggaagcact cattttaaac aagatgaaga
     8881 tgccttagat acttggttca gttctgcatt atggcctttc tctactctag gatggccaaa
     8941 taagactgaa gctttagatt attattatcc aacaagtgta cttgttacag gatacgatat
     9001 aatattcttc tgggtagtta gaatggcatt tgcaggtatg ttctgtatga atgaaaagcc
     9061 atttgaccat gttttggttc atggattagt aagagattct caaggtagaa aaatgagtaa
     9121 atctttagga aatggtatcg acccattgga gataatcgag caatatggag ctgatgccct
     9181 aagatttaca ttaacaactg gaaattcccc aggaaatgat atgagattct atatggaaag
     9241 agtagaattt gctagaaact ttgcaaataa gttgtggaat gcatcaagat ttgtatttat
     9301 gaatattgat gaagatataa ttaaaaacat gactagagaa agtgttaaag aggacttaac
     9361 tctagctgat aaatggataa tatcaagagc aaataatatt gtaaaagaag caactaataa
     9421 catggataag tttgatttag gaatagcact tcaaaagata tacgatttta cttggtcaga
     9481 atactgtgac tggtatattg aaatggttaa acctagatta tatggagaag atgcaaatgc
     9541 taaatctgca gcattatata cattaactta tgttcttgaa aaaatattaa aattattaca
     9601 tccatacatg ccatttataa cagaggaaat atatactcat cttcctactg ttgaaggatg
     9661 tataatagta tctgaatggc caaaatataa tgaagaagat aacatggctg aagaagaaga
     9721 tatgatgaac ttattgatgg aaggtataag aagtataaga aatgtgagag cagaaatgaa
     9781 tgtaccacct tctaagaaag ctaaattgat aataatccca agtgaagaaa aaatagaagc
     9841 tattgagctt ggtaaagact attttataac tttagcatct gcttcaaatg tagagatagc
     9901 aaaagataaa tctaatgttc cagaagatgc agttggagta gttattgatg gtgttgaaat
     9961 atttatacct cttaatgaat tagttgattt tgaaaaagaa attgagagat tatctaaaga
    10021 aaagaaaaag ttagaaggcg aaataaagag agttaatgga aagcttgcaa atcaaggttt
    10081 cttagcaaaa gctcctgaaa gtttaattga agaagaaaaa gcaaaaaaag aaaaatttga
    10141 agagatgatt aaatctgttg aggaaagatt aacaaattta gaatctaaaa taaaataaat
    10201 ataagaatta gtaaaaacat acaaaaagta tatattctat actttttgta tgtttttctt
    10261 tatatatata caaata
//
LOCUS       DAYAOX010000131        10096 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.131, whole genome shotgun sequence.
ACCESSION   DAYAOX010000131 DAYAOX010000000
VERSION     DAYAOX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.131"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            149..1300
                     /locus_tag="ACGI57_002549"
     CDS             149..1300
                     /locus_tag="ACGI57_002549"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9717619.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVISDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1418..2056
                     /locus_tag="ACGI57_002550"
     CDS             1418..2056
                     /locus_tag="ACGI57_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HFG9717620.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2057..3079
                     /locus_tag="ACGI57_002551"
     CDS             2057..3079
                     /locus_tag="ACGI57_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFG9717621.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            3069..3689
                     /locus_tag="ACGI57_002552"
     CDS             3069..3689
                     /locus_tag="ACGI57_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717622.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3813..4499)
                     /locus_tag="ACGI57_002553"
     CDS             complement(3813..4499)
                     /locus_tag="ACGI57_002553"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFG9717623.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQNKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4513..5058)
                     /locus_tag="ACGI57_002554"
     CDS             complement(4513..5058)
                     /locus_tag="ACGI57_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9717624.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5286..6533
                     /locus_tag="ACGI57_002555"
     CDS             5286..6533
                     /locus_tag="ACGI57_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFG9717625.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLTFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6620..8614
                     /locus_tag="ACGI57_002556"
     CDS             6620..8614
                     /locus_tag="ACGI57_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9717626.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8614..9936
                     /locus_tag="ACGI57_002557"
     CDS             8614..9936
                     /locus_tag="ACGI57_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFG9717627.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
BASE COUNT         4108 a          990 c         1666 g         3332 t
ORIGIN      
        1 taaattaggt ttatatttta taaaaatttg ctaaaaaaaa caattaaaaa ttgtaaagaa
       61 aaaagatata attagaactg tagtaataaa tgttttatta tagataatag agttttaagt
      121 atcttaaata atatttggag gaatatgaat ggaattaaga ccaagaagtg caaaattaga
      181 agaatttgat aaagtaatag aattaataaa ctatgtattt agaatatcaa gaaatcataa
      241 accaactatg atggaagaat ttcctctttt actaagtaaa aataatatag aaaatatgat
      301 tataatatca gaagatgata aagtgatttc tgatgtaaac tatctaattc aggatgtatc
      361 tattcaagga aacagattga aggttgcagc tataggtgga gtatgtactc atccagatta
      421 tgaaaagaga ggctattcat ctaagatatt ggataaagta gaagaaaaaa tgttttatga
      481 tggtgtagat attgtaatta tatcaggaac aagaagtcta tacagtagaa gaaattgtag
      541 tttggtaaag agtttttata aatatacaat aaaacctgaa gatgtaaaaa tagcatatga
      601 aatagttgaa tttgatgaaa ctaattttga aaaagataat gatttagata aaatgattga
      661 actatataat caaaatagta ctagatttat aagaacgaga gatgagtttc aaaaactatt
      721 gcatgcagca actattgcct ggggaccaat tggttacaag aaggtattta taaaggaaaa
      781 taataatata ataggatatt tgataataag aacaataaaa aaagaagatt ctactgtagg
      841 agaagttgct gaaatagggt taaactctgt aaatgttgaa aatatattga aatatgttgc
      901 aaataaattt ggattagaat atttaaatta taaagtacat gtgaaaaatc ttaaagacca
      961 gttgaaatgt aatgggacaa aatctttgga ttatcaacaa ggaactatga aaataataaa
     1021 ttttactaag ttgtgtgata gtttaagaag ttattttagt caatatgtag attttgaatt
     1081 actaaaatac atggaattta aacaagttga aaataaatat attattaaat acaaagaaga
     1141 agaacttgta atagaaaatt tggataagct taataaactg ttttttgaaa aaaatgagga
     1201 acagtacaat gaatttaaac atctaaaaaa tatatatgaa tttgctacaa aggcatttcc
     1261 tgtagatttt ccatggactg caaatttaaa ttatcaatag ttttagatga tattaaataa
     1321 aattgattga tacctagagt tagtccttgt taaataagag aaattattgg atattgaaaa
     1381 agcaattttg taagtaatta gaaaggatga gtattatatg gacatacttg atgatatagc
     1441 agaatgtata ttaaatatgg gtgtagataa tatagagaat ttagtaaaaa ttgctataga
     1501 taaaaatatt gatgtggaag atatttatga atatggttta aacaaaggta tgataggagc
     1561 tttagataag tttgagaata aggaatacta tctttcagaa gttatagtat gcacagatgc
     1621 attaaataaa ggtattaatc ttttgaaggg aactggaaaa gtaaagaaaa aatcaaaggg
     1681 agtaattctg atgtctgtag tagaaggaga tactcatgag ataggaaaaa atattgtaaa
     1741 agtaatggta gaagccactg gatataaggt tattgactta ggtgtaaata gaaaaagtga
     1801 agatataata gaagaagcta taaaaaacaa tgtagatata ataggcttat cttcaatgat
     1861 gacaactaca atggaaaata tgaaatcggt tatagacgaa cttaatatga ttgagataga
     1921 taaaagacca aaagtaataa ttggtggagg acctgtaagt atggagtttg cagaagaaat
     1981 aggagcagat ggatacagtt caaatgctcc aaaagcagtt aaattaataa ataaacttat
     2041 aggaggtgaa gtttaaatgt tttcagatat gactttactt cagtatataa atagtgaaga
     2101 tagaggtttt tttctgcctg atatgggtac aaatggattg ttcttaataa ataaaaaggc
     2161 atatgaagta tatgatagtg tagatttgca attagaatta gcaaagacta tggacaagta
     2221 ttttgaatca gattttatat actcattctg tgatgggatt acattttgtg aaactctggg
     2281 tttagagaca ttaagaccag attatgattt tccaagtgta ttgacacata ctataactga
     2341 cagagataaa ttaagaaaat tagatgttcc agacccttat acgagtggac gtatgccact
     2401 caatatagag agcttatcag ttatatcaaa aagtattaaa aaaccactat atgaatcgat
     2461 acaaggtcca tttacacttg caggtcaatt agctggagct actcaacttt taagatgtat
     2521 aataactgat aaaaaatttg tagaagaatt attggaattt acaactgaat tggtaagaag
     2581 atatgctgta gctgcaaata aagcaggagc aaaatatatt tcaatagcag agccaacatc
     2641 agtaacccta agtaaagata gatttgatga atttatagta aaaaatttaa ataaaatcta
     2701 caatgaactt gattgttgga aaggtatgca tatatgtgga gacaccagag aattattaga
     2761 taatatgcta agttgtaata tagatgcagt aagtttagac caaattttag actatgaaga
     2821 aattgctcca atgattccta gagatatagt tttgattggt aatttagacc caataaaact
     2881 tcttggacgt tctaagccag ataagattag aagagagacg ctaaaattgt taaagaagat
     2941 gagaagatat gataactttt tatgtgattt tggctgtaac tgcttaaata ccactccagt
     3001 agaaaattta caagcagcta taaaggcagg aagaattagt tataaagaat tagataggat
     3061 tgatattgat gaattataat agtaataaaa ataaatatct tttaaaggat ataaatttag
     3121 atgatatttt tatagataag aatattgtgc taaagttttt ggggtatgga aatagaaaag
     3181 ctccagatac aattagtaaa atcgttgaaa gagaaataag aaatataaat gatattttag
     3241 atatcaagat atatataagt gaagttgata ttaataaaac acctaaagaa tgtaaaaaag
     3301 catttgctgt actatataca ataggggata aaattgatat taagatgaat gactatatgg
     3361 aaaattgcaa tatgatggca gggctagcat tggataaaat aggtatagta tgtttagatt
     3421 atattaatga gaaaataaaa atgtatttaa aaggaaagta tgatagtctc aaaatatcgc
     3481 atgaaatata tccaggagat aaagattttg aagtagagag acaaaaggat atttataact
     3541 atgtaaaaaa taaatataat agtatagaaa tagaaattaa tgattatcat caactatccc
     3601 ccataaaaag tgttgcaatg cttattttaa tgggggatga agaaaattta gaatcaagat
     3661 gtagtaagtg tcctagaaaa tgcttttagt taattttcaa gactaagatt tgttttgtgt
     3721 ataaattgca ataatttttt tggtcatttt atgtataaac taaaaaaatt attgcaaagt
     3781 aatattctat ttaatttgaa cagacttaca aactatagta gtttctttaa ttcaataagt
     3841 gtatttattt cataagttgg tatatgatta gtataattta tactgttatt ggaatttaac
     3901 caacaagtat ctattcctga atttattcca cctaagacat cagaggttaa gctatcccca
     3961 attattaagg cagaagattt atcatgacaa ttaaactttt tgagagcgta tttaaatatt
     4021 tcaggatttg gttttgatat tttcaactct tcagatacaa ccattccatc aatatatcct
     4081 tttatttttg aattttcaag tcttttattt tgaactgcag aaattccatt tgttattata
     4141 acaatagtat actttttttt aagatattgt aatatatcga ttgcacctgg aattaaaaat
     4201 gtacattcac ctaaaaaatt taagtacatt ttgctaagag tttcagaatc aacatccaaa
     4261 gatattttat ttgcaaaaag ttcaaatcta agtaatttaa gttcattcaa agttataata
     4321 tttttttcaa ggtcgagcca aagcttatca cttatatttc tatatgactc tatataattc
     4381 tcaaaattaa attctaaatc aaactcagat agaagttttt taaaagcatg tgattcactt
     4441 ttcttaaaat caaataaagt atcatctgca tcaaaaaata taaatttata tgtcttcatg
     4501 gtttttactc ctttacaaga tgactgcaaa aattacttta ctttcactat ttgagtcatt
     4561 ttgccattta tgttttgttc ctaaaggaac ttttacacta tctcctttgg acaaagtaaa
     4621 ttcatcatca tccataaaaa gtttaacttc tccctccaaa acatatgcaa tttcatctcc
     4681 attatgagta atcctatcaa cacaagaaga agtgtttgga gctaaatcca taattgcaaa
     4741 ttctaaatta ccactagagt ctggaattaa aatctcatat ataacttctt tgcttccagg
     4801 taaaataact ttttttctat tatcatgtct tactatcaag ttttttttat ctacttcaga
     4861 tacaaaaaat gtaaataaag gaacatttag agaagaggct atagatttta atgaatttac
     4921 agatggatta gcaagacctc gttctatttg gctcaataat gatggtgtaa catcagctag
     4981 ttttgctaaa tctcgaatac ttaagttttg tttttttcga acttcagcta ttttttcccc
     5041 taaatttaaa tcattcatat aaatatcctc tctttttaat ttaaaaaata ttaacttaaa
     5101 ataatcaagt aaaatgatta tgtagtatga aattattata acatatagaa ttaggttttg
     5161 aaaaaactat gttcaatata attaaataaa ttaaaatata attgacaaag tggatacaga
     5221 atacttaaaa tatatttaaa taaaattaag caaaagatag ttaatttatg ataggagggg
     5281 atattatgca aataacgact tttggagcta tatttggttt attaatagca attattttaa
     5341 ttataaaaaa atttcaagct gtatatagtt taatgttagg agcatttatt ggaggattag
     5401 ttggaggagc aaatataaca caaactgtag attttatggc aaatggagct atgaatatat
     5461 caccatcaat tcttagagct ttggcatcag gtgtattagc tggaagttta ataaaaacag
     5521 gggcagtaga taaaatatca gaacagattg taaaaatatt tggtgaaaag agggctttat
     5581 tttcgattgc catttcaact atggtacttg ctggagtagg tgtaaatttg gatgtatcta
     5641 taataactgt agcacctata ggattatata taggtagaaa attaaattat tctaaattgt
     5701 caatactatt agctatgttg ggagggggta aagcaggtaa tataatatca ccaaatccaa
     5761 atactatagc agtagcagat aatttttctg tcaatctctc tagtgttatg atggcaaata
     5821 taattccagc aattattgga gttgttatta cagttatttt agcaagtata ttaataaata
     5881 aaggaaataa agtacaatca tatgaaattt tagagcaaag agaagattta ccaagtttat
     5941 ttaaatcatt atgtggtcca attatagcta tatttctatt gtttttagga aatgtatctc
     6001 ctatagttat agacccaatg atagcccttc caataggagg aatcgtaact ctaattgtca
     6061 caggaaactt gaacaattca agagaatatt taacttttgg attatctaag atgcaagggg
     6121 tatgcatatt attacttgga acagggacta tcgcaggaat tatacagatg tctgaattac
     6181 aacagagtac aataggtgct ttacaatttt taaatatgcc acaattttta ttggcaccag
     6241 tttcgggtat acttatgtca ttagcaacag catcatcaac agctggtgct actatagcat
     6301 catctacttt tcatgatgct ataataaatg gtgggctatc accgatttca ggtgcatcaa
     6361 tagtaaatgc tgggtctagt gtattcgagc aattaccaca tggctcacta tttcatacta
     6421 gtgcaggtag tataaacatg gatataggtg aaagatttaa gttaattcca tatgaagcat
     6481 tgataggaat agtaatgact ataatttcaa catctattca attagtttta taataaatat
     6541 aaatatttaa tgaaaaaagt aggattttgt tattaaatat tgaacttatt atagtaaaaa
     6601 agaagattgg agttttaaag tgaataaaaa aaagattgtt attataggga ttatttattc
     6661 atttttagta gtattttcac ttacaaatat gtatgtaaat atggagtata atctaaatgt
     6721 atttgaatat attaagaagt cacttccatt tacagaagaa gaaaaaaagt ggttagaaaa
     6781 acacaagaat ttgatttaca gctctgacca aagttcacct cctttgagat ataaaggtaa
     6841 ggaagatgga cagtataagg gaattattgt tgatttaata aattctctgt caattcaaat
     6901 tggtagagat ttttatttta aaccaaataa ttggtggaaa gaatctttcg taaattcaat
     6961 agatgatagt ataaagtttt ttgacttgat tccatcaaaa gaaagggcaa ataaatttat
     7021 atttactgac ccaatataca ctttgagtgc aaatatattg aaagataaaa agtcccaaga
     7081 tataaatagt tacatggatt taaaggggaa aacagtagca attccagaag gtgattattc
     7141 tataaatttt ttaaagcaaa aaattcaaga tataaacatt ttattgactc cagatataaa
     7201 gactggagtc aaccatttaa tgtctggaaa agtagacgca gttgttggtg atgaaccagt
     7261 attgcgatac tatataaata attatggact ttcaaataaa tacagtgttt taagtaatcc
     7321 tatatatact aaaaaagcag ttcttgctgt acctaaacaa tatgaagagc tggttagtat
     7381 tttaaataaa ggcattttta aacttcagaa aaatggagtg tataaagatt taaagaaaaa
     7441 atggtattct acgtataatg aagttgatga tatattgtat gagcgaggaa ttgtgcctag
     7501 tatttatctt tttataggta taattttaat atcaatttat gttttttata gctatacata
     7561 tttgttaaag atagaaatca aaaggaaaac agaacaggta atagaaaata aaaaaacttt
     7621 agaggcaact tttaatagta ttacagatat aataatgctt gtagatgaaa ataacaatat
     7681 agtagaatcg aacaaagtac tatatgactt tatgggtgaa atgagttata agatagcaga
     7741 tttgattagc atgataaaag gtgttattga aaataccttc tctgaaaata ctaataaaac
     7801 tagtgaaata gaaatacaca ataaaatttt aaagataaac acttttcctg tagagtataa
     7861 aaaaaataat actgagtata tagtagttct tattaaagat ataactaatg ataaaatagt
     7921 agaggcgaaa ttacttagag aaaacaaaat gatatctata gggcaattag cttctggggt
     7981 agcacatgag ataagaaatc ctttgggtat aataagaaat aattgctatc tcttaaaaga
     8041 taacgttaca atggaagaag taaatgattg tgttaaatca atagaaagta atgtagatag
     8101 ggcaagtaat ataataacca acttgttaaa tttcgcaaga atatcagatg ataatttaga
     8161 acatataaat ataaaaaatt ttattgaaaa tatagtgaaa cttcaatata aaatgttaca
     8221 gttaaagaat gttgaaataa aaattgactg tgaacataat ttaatatgct atataaatgg
     8281 agaatcgtta aaacatgtat ttataaattt aatttctaat tcaatagatg caatacatca
     8341 agatggaaaa attattatat actgttatga aaaaaatcat tgtttattta tagattttaa
     8401 agataatggt gaaggaataa aagaagatgt cctaaaggac atatttaatc ccttctatac
     8461 aacaaaacca attggtgagg gaactggttt aggtttatat attacttata atgagattaa
     8521 aaagaataat ggagatataa gtgttgaaag taaattgggg gttggaactt gttttcatat
     8581 aaaaattcca ttaaataaag aggtgactat ataatgaaaa tactattagt tgatgatgag
     8641 ctagaatatg gagtggtaat gaaaaaaatt ctacaaaaga agggctatct tgtagatata
     8701 gcactaagtg gtgaagaagc tataaatata attaaaaaag ataaaaatta tgatttggtt
     8761 ttatctgatg ttatgatgaa aaatatggat ggagtacagc tattagatag aattaaatct
     8821 attaatagag atatagaggt tatattagtt acaggatatg gaagtattga aaatgcagtt
     8881 gatgctatga aaagaggagc tttatcttat tttataaaaa gtaatcccat tgaaaattta
     8941 ttagaagaag ttgaaaaggt aaaggcaagt aaaatatctg tttctgtaca aaaaaataat
     9001 tttgaattta ctttggaatc taaaaataga gattttaata atatcataaa aatagcaaaa
     9061 aaagctgctt gtaaagatgt taatatatta atcttggggg aatctggtgt aggaaaagat
     9121 atattagcta gatatataca tagcataagt ccaagaaaga atgagatatt tgtacctgta
     9181 aattgttgtt ctttttcaga aaatctattg gagtctgagc tgtttggtca tgaaaaaggt
     9241 tcttttacag gagcagtaga tagtagaaaa ggtagatttg aactttcgaa taaaggaact
     9301 ctatttttgg atgaaatagg agatatacct ctaaatgttc aggtaaaact tttgaggaca
     9361 cttgaggaca aatctataga gaggatagga agtaataaat ctattaaagt tgactttagg
     9421 ttaatttgtg ctatgaataa agaaccaaaa gtggaaatat caaatggaaa tataagagaa
     9481 gatttttttt atagaataag tacaataaca ataactatac caccactaag aaagagaaga
     9541 gaagacttaa ctactcttat agaatttttc ttaaataagt accaaattga acatgataaa
     9601 aagatacact cgattgataa ggaagtaaag gattttttat taaattacaa ttatccagga
     9661 aatataagag aattaaaaaa tataataaat agacttgtag ttttaagtga agaaggtaat
     9721 ttatcaaaag acaatttaaa tttaatatca aataatgtat acatagatga taaaataagt
     9781 attagacctt taagagaaat aagaaaagag tttgaatgtg agtatattga aaaagtttta
     9841 tctttgtgtg gaaataatat ttcaaataca gctaaaaaat tggagattag tagaagacag
     9901 ttaaccaata aaatatcaga gtataatatt aagtgagaaa aaaacttctc attaagtgag
     9961 aaaaaaattt ctcaaactgt attaaaagat aaacagaaaa aatctgttta tcttttttta
    10021 ttataatttt aaaaattaat atttacaata attattgcta aattcaatat tggtgtaaat
    10081 taaaaatgaa aatttc
//
LOCUS       DAYAOX010000132         9963 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.132, whole genome shotgun sequence.
ACCESSION   DAYAOX010000132 DAYAOX010000000
VERSION     DAYAOX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.132"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..1413)
                     /locus_tag="ACGI57_002558"
     CDS             complement(<1..1413)
                     /locus_tag="ACGI57_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9717628.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYN"
     gene            1554..2450
                     /locus_tag="ACGI57_002559"
     CDS             1554..2450
                     /locus_tag="ACGI57_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFG9717629.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASHVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHKEVSNVILKEDMK"
     gene            3115..3255
                     /locus_tag="ACGI57_002560"
     CDS             3115..3255
                     /locus_tag="ACGI57_002560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717630.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(3252..3623)
                     /locus_tag="ACGI57_002561"
     CDS             complement(3252..3623)
                     /locus_tag="ACGI57_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9717631.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            4172..4429
                     /locus_tag="ACGI57_002562"
     CDS             4172..4429
                     /locus_tag="ACGI57_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717632.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(4539..5219)
                     /locus_tag="ACGI57_002563"
     CDS             complement(4539..5219)
                     /locus_tag="ACGI57_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFG9717633.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            5479..5778
                     /locus_tag="ACGI57_002564"
     CDS             5479..5778
                     /locus_tag="ACGI57_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717634.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            6102..7757
                     /gene="ilvD"
                     /locus_tag="ACGI57_002565"
     CDS             6102..7757
                     /gene="ilvD"
                     /locus_tag="ACGI57_002565"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFG9717635.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            8101..8730
                     /locus_tag="ACGI57_002566"
     CDS             8101..8730
                     /locus_tag="ACGI57_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9717636.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            8756..9568
                     /locus_tag="ACGI57_002567"
     CDS             8756..9568
                     /locus_tag="ACGI57_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9717637.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         3986 a         1107 c         1483 g         3387 t
ORIGIN      
        1 attatattta aataaataaa agccaattaa tattgcacct attaagcaaa caagtgtaac
       61 aataatataa acatttctca ttccaaatac aaatatttta tcattattaa tcacataatc
      121 attgacccta tatccagctt taatactcat aaaattatat aaaagtgttg tagaaatagt
      181 aatacctata acttgtccta aatttcttac aagggaattt atgcttccta caactcctag
      241 cttacttcta gaacagcttg acattatcaa agcattgtta gcaggttgaa atatagcttg
      301 tcctattgat attatcgata tataaattac aaataaaata agggcagaac tttctttcaa
      361 tcttgacatt agaaagaatc caaaactcat aacgagaaga ccaattaaaa taattttttc
      421 tgacttaatt ttatctgata tattcccaaa tataggtgaa aaaatggcta aaatcaaagg
      481 tgatagtatc ataaataaac ctgcttgaat agggggaagt ttcatagtac tttgtaaata
      541 aaatggaatt aaaatacttg atgatgcaat acaaatgaat gatgtaagag cacaaattaa
      601 attcaatgaa aatttaaaat ttctaaaaat ttcaaggtca agaagcggtt gaggatgttt
      661 tttttctaat atcaaaaata atataataaa aataattgat aataatagtg caattaatat
      721 atatggattt agtaatcctg tttgttgagc actaattaga gcactaaaaa ataatgttgt
      781 tcctaaaaat tgaagtatag caccagtttt atccatattt tctgaagatt ttttactatt
      841 aggaagaaat tttgtattta aaaagattac aataagaccg attggaatat ttataaaaaa
      901 tataacattc caatcaaata ttgacaaaat aaatcctccc acagatggtc caaccatatt
      961 tccaatagca actgcagtaa ctaaaatccc tagggctttt cctctgcttt cttttggaaa
     1021 tagttctgtg ataattccat gattgtttgc catgtaggca gaagcgccta ttccttgaat
     1081 aaagcggcaa actataagta aagttaaaga attacttaat ccacatagta atgaagctga
     1141 tgtaaagaga atagtaccta cttgaaatac tcttgattta ccaataatat ctccaagtct
     1201 tccaaaaaat aatagtgttg aacaaataat aattgagtaa cttgctatta cccattcaat
     1261 agatgctaga gatacattta gtttgcttgc aagaactggg agagcaacat ttataatact
     1321 agaatctaat gttgccataa aagtcatggc aatgactaca aataaaactg tgattttttt
     1381 tctattatca tcttttttaa aagagtttgt catttttatt acctccttat tataatgact
     1441 gcattatgtt aatgtttaca atgatagtta cattttatat aatagcgata tgaaataatg
     1501 aatacaataa ctatattgct tataattagc actattttga tgagggggga ataatgaagg
     1561 ataaaaatac atattttaac aggaaaggtc aagaaaactt tatagatatt tttttaaagg
     1621 atgacattaa aaaaggagca aactttaaaa gacattggca tgagcatcta caaatatatt
     1681 attttattga tggtatagca tatcttgaat gtggattgga taattttaag gtagtgcctg
     1741 gaaatattgt aatagcaaac agtaatgaat tacattattt agaaagctta tcagataatt
     1801 taaaattcta tagtattcgt attgaaccca cattcctttt tagtaatcaa gttgatttgt
     1861 tacaaaccaa gtacttagca ccattggctt taaatcatat tgtatttaat aatttaattc
     1921 aaaatgattc tctgatttta aaatgtatta caactatact tgatgaatat cacaataaag
     1981 aagttggata tgagttatct ataaaagcta atatctatca attaatagta ctcttattaa
     2041 gaagtcatat tgataaaata tattctgaag atgaatttat aaaaagaaag tattctttag
     2101 aaaaatttga atgtatcttt gatttgatag aagaaaaata tatggataaa attacattaa
     2161 aagaacttgc tagtcatgtg aacttgagtg cacatcactt ttgtagaaca tttaagcaaa
     2221 taacaggtaa gactacaatc gattttataa atggaatcag gcttgataaa gctatatatt
     2281 atttaaagga aacagattta aatatgacag aaattgctat gagatgtggc tttgatagca
     2341 taaattattt tagtcgctta tttaaaagga attataatat atctccaact aagtatagaa
     2401 atgaacataa agaagttagt aatgttattt taaaagaaga tatgaaataa ttcataaata
     2461 tactttatag aaaatatata tttttaaatc tttgagtttt tcttaataaa tattaataaa
     2521 gtatagtaca taaatgagta agactacttg tatagttaaa tgtgaaaatg agtatgaaag
     2581 aatgtagtgt ctatgaaaag tatattttaa tatgaaacta aaattggaaa aataagaata
     2641 gcagaaaaaa tgattatatt ataaatcagt ttttttgaga catctggctt agattctgaa
     2701 agatatttaa tcaaagaaat aaaactatta aataagcaag taaacagcta aaatagtatg
     2761 atttttatag aaaatttaaa tccatcctaa agaattaaaa gaaagtcaaa taaatataaa
     2821 aaaattatat atttataaaa aattttataa taaaaatcat cctgaaaaat atataaaaat
     2881 agaaaaaaca tacaagattc tacaataata gatttttttg ttattattac gagtttttta
     2941 ttgcaactgt agaataaaag tattattata taattaagat aattatctag ggaaattttg
     3001 gataatcaaa atatatttta taaaatcaaa atacaattac ataaacttta ttaaaaattt
     3061 gttgggaatt tctgactaac attaagaacg taagtttgtt gggggatggt atatgtggaa
     3121 aagtcaaaca ctactgttac aaaaaaagaa aaaattgaag atataggaat gaaattagaa
     3181 catttattga gtgaagatat tactttttat gaattattac ttgagatttt gtataaagaa
     3241 aaagaagaga attaattctc ttctttaagg aatcttttta aataggcatt atcaatcatt
     3301 ttttctatag cttctctatc ttctttttca agagaatata ttttttctaa gagctttttt
     3361 gttttttcac ttactgataa gttctctatg attaatttat cttctttgct tatttttgta
     3421 ttatcattta cgcctaataa ataatctgtt gatgtttcta atgcttttgc taatctaact
     3481 attatttcac ttttaggttc tcttaaatta ttttcataac gtgacaagct agcctcagtt
     3541 ataccgactt tagtagcaag ttctttttga tttatatcta attcttttcg tagatttgct
     3601 attctatctc ctaaactttc catatgtaca tcactcctta ccgaaattat aagttatttt
     3661 gataaaaagt aaaataaaat taccaaaaat ataaaaaaaa aaggttaaaa taataaaata
     3721 tattgaaaat taccatttgt atatgtataa tatgaattat acaaatggta atttgatgag
     3781 aggtggtatg ttgaaataag aaactgaatt aattaataaa tacatagaaa gttttgaaaa
     3841 cctttaattg agatataaac aatatgtaca aaattttttg caagtaaaat taccaaatgt
     3901 ataaaataaa tcagtagcat agataatttt atgttttaat acattagatt atttaattta
     3961 gttataatgg tcaaaaatgc aatctacaaa taaaacaaag tattatcaaa aaagtattct
     4021 ttaagaaaat tttttattag agatgtaatt gatataaata atcgtattaa aaaataaaat
     4081 actaagttta aaaatttaaa taatctataa atcaattata aaatattatt tattagaaat
     4141 taaaatgaat taagtaaatg gaggaattga tatgataata attagaagtc aagataaaac
     4201 taatttaatg cagataaacc aaattaaaat agatggtagt caagtttatg cagtatttga
     4261 aagtaaaatt gatactgtaa aaataggaga ttatgaaaat aatacaagag ctatacaagt
     4321 gttggacaat atacaaaact tcattgaaaa tggaacaaaa tatgattata taacatcaaa
     4381 taaagtcaga tacaatgtta ataaaatatt tcagatgcca gctaaatagt gagtgtgcat
     4441 tttaatttaa taactaaagc ccactttctt ttgtagtatg aaagagggct ttacatattt
     4501 gtaaatatcg aagtacactt attttatcaa taaattattt aataaggtat aatggaccaa
     4561 ttaaacaata aaaattctcc taaaacagca agtattaaat atataatagc ttcatttttg
     4621 taaagagagg taattttttt gcttatgtca tcatcaattg taatagcaat ctcatcttga
     4681 ataatatttt tatatataga tgggaaaata tcttttttct ttataagatt aaataaatta
     4741 tttacaccta cccatataaa atatcctaaa acagcaccta atgtgttcat cattaaatca
     4801 tcaatatctg tagctcttaa gttaaacaat tgacaaagtt caattgctaa tgaaaaacct
     4861 aatccagtta atgaaacatt gataatattt ctaaaatttt tccaaattaa aggtagtaaa
     4921 aatcctaaag gcataaacat aattatattt aaaatatatg ttaagatgcc aactgagtca
     4981 aaaggtatta gatttatttc atcaattcta aaaagcccac tataccttcc tatatcccat
     5041 atgctaccaa ttccagcttt actaagtgct agatagatat ataataaaaa aatatatacc
     5101 catactaaat gtatcattac attcttttca actttcaaat tttttcgttt tataacgact
     5161 atttggtata ttaagcatgg taatataacg caaataaatg aataaaatgt ccatatcatt
     5221 ttattttgtc ccctttcaaa tttatgtttt tagttttata agtactatct ttatattaaa
     5281 ctgtaagttt taaaaatctt ttaataaaat cttaaaaatt tattaattta taaaaatata
     5341 ttattaagat taaaaaatat atataatcaa agtttctaca attatatagt tagggatata
     5401 aaaattttat cgtatgcata aatattataa atatatggaa ataataatag tatattttgg
     5461 taaagtagga ggaattttat gaaatggatt aaatggtata gtattacatg catatgtatt
     5521 tttatagtag tagcttttta tatgtttata tttcctaata aaatagaaac tattgatact
     5581 tcatctgcat atagctttgt tgaaaagaaa gtaccaaata gtgctgtgta tcaaggatac
     5641 aaaaagaatc cagttgatgg tactactaca atttattaca gctatgataa ttcaactcat
     5701 atagttagat tatcacatcc agaagactat tcaaggaaga ttaattggga taaagtttca
     5761 aacatcagat ttgattaatc tttaatattt ataaaagtct atatatctaa ggcaaatata
     5821 ttattaatct tattcatagt tgagcacttt ctattataga agtgcctctt ctgatttata
     5881 tcataaaata ttactcaaca atttgtaaaa tatattagtt tataaaatat attgaaaaga
     5941 taatattata taatacgatt gtgtaagata ttgataaaaa atcgtaataa attttcatta
     6001 gtatatgcta atattttttt acaattttcg taattaatta atagaaaaat atattataat
     6061 aatattatga aaatttaaaa aagtaagaaa gaggtaatat tatgagaagt gatataaaaa
     6121 aaggaataga aggagctcca aagagagcgt taatgtatgg aatgggtctt actaaagaag
     6181 aaattgaaag accattaata ggtatagtaa atgcacaaaa tgaggtgatt cctggtcatc
     6241 tgcatcttga tgaaatagca gaagctgcaa aaaatggagt aagaatgagt ggtggtttgc
     6301 cattagaatt tcctgctata ggggtatgtg atggtatagc tatggggcat gtcggaatga
     6361 attattccct ggcttcaaga gaattaatag cagattcaat agaagctatg gctatggcac
     6421 atggttttga tgctttggta cttataccta attgtgacaa gatagtgcca ggtatgttaa
     6481 tggctgctgc aagacttaat ataccaagta ttgttgtgag tggagggcct atgcttccag
     6541 gtaaaaaaaa tggaaaagta tatgatttta actctgcaat ggagggtgtt ggagcatgta
     6601 aagatggaac agtaagtgaa gaagaattag aagagttagc catgaactct tgtcctggat
     6661 gtggttcatg ttctggtctt ttcacagcaa atagcatgaa ttgtttaaca gaagctttag
     6721 gcatgggtat accatataat ggtactgctg cgtcacattc tggagaaaga aaaaggatag
     6781 caaaatatgc aggtatgtat gttatggagt tacttaagaa cgacataaaa cctagagata
     6841 ttttaacaat agatgctttt aaaaatgcta tagctgtgga tatggcaatg gctggttcta
     6901 caaatacagt acttcactta cctgcaatag cttatgaatc aggaatagag cttaacttag
     6961 atttttttga tgaaataagt gaaaaaactc cttgtttaac aaaattaagt ccaagtggaa
     7021 aacatcatat tgaagattta catatggcag gaggaatacc agctataatg aacgaacttt
     7081 caaagataaa tggaataaat ttagattgca aaaccgtaac aggcaagact ataggggaaa
     7141 atataagaaa ttgtgaaata gaaaatgaag aagtaataca tacattaaag aatccatata
     7201 gtaaccaagg tgggcttgca atattgaaag gaaatcttgc tctaaatgga gctgttgtaa
     7261 aaaagtcagc agttgcagaa gaaatgttag ttcatgaagg accagcaaga gtttttaatt
     7321 cagaagaaga agctgtaaat gctatttttg gtaaaaaaat aaacaaaggt gatgttatag
     7381 ttataagata tgaaggtcca aagggtggtc caggaatgaa agaaatgcta tctcctacat
     7441 cagcagttgc aggaatggga cttgataagc atgtagcact tcttactgat ggtcgttttt
     7501 caggggcaac tagaggggca tccataggcc atatttctcc agaagctatg gaaggtggtt
     7561 taatcggact agttgaagaa ggagatataa tttctataaa cataccagac aaaaaattag
     7621 agttaaaagt agatgaagtt gaaatagaaa atagaaaatt aaaatttaaa cctttagaac
     7681 cgaaaataaa gcatggatac ttaagtagat atgctaaact ggtaacatca gcaaatacag
     7741 gagcagtttt aaaatagtag actatattta tgtaaattag aaaaagttaa gaaaatatta
     7801 attggatatc ttaggtgtta tagcactaat gaggtatcct ttttattttg tatgaaaaac
     7861 atagcaaaat cttgattatc tttgaaaaca atacatctct attataagga atacgagtac
     7921 agcatagaga tgtatctttt agaccttgtt gaggtaatac aactttttaa aagtttgatg
     7981 tggaatgctt ttcgttcctt ctgaactatg aagaaattga aagtaagtta attcttgaca
     8041 taataaatat taaaatgtat aataaaaaac aaacagacag tctgttttta ggagggaaat
     8101 atgaatagag aagaaaaaag taaaaatagt aaagaaaaaa ttatccaatc agcattttca
     8161 ctattttcgt ctaaaggata tgattcaaca tctacacaag atattatcaa tttatctggt
     8221 ctatctagag gtgcaatgta tcatcacttt aaaactaaag aagatatact gagaagtgtc
     8281 acaaaagaac tttactcaca aatgaataat tttttagagc atcttgttgc tgatgacacc
     8341 cttacagcaa atgaaaaaat aatagaattg gttgttcata gtgcgaatga ttacacacgt
     8401 agaaaaatgg tacattgtag ctggttagaa aaaatcccat tcgctttaat agaggaagtt
     8461 cgtaatctca acaatgtagt tgcacccaat attgctaaga taattaaaca aggtgttgaa
     8521 aataaagaat tttcttgtga atatccagag gaattagctg aaatgctcgt ttttagtatt
     8581 gatattttac ttgaccctgt attatttaaa cgtgaataca gtgaagtatg taataggttg
     8641 gattttttat tattcatgtt aaaaaaaatg gatattcctc taattgatga gtatggtatt
     8701 caaaaattta aagatttatt taaacaatga taataggaaa agtgaggaat agactatgtt
     8761 ttataatgca aaaagttgtc aaatgatgat aggaagtaat acagtgaatt atattgaatt
     8821 tggtagtggt aagaagacac taattatttt accaggatta ggagatggtt tatttccttt
     8881 acatggaaaa atacaggcga ttgcttttgc ttttagatat aagcaattta caaaagatta
     8941 taaggtttat gtatttagta gaaaaaatca aattacagaa aaatattcca caagggatat
     9001 ggctaaagac caagcagata taatgaaaaa acttggaatt atgaaagcag aagtaatggg
     9061 tgtttctcaa ggtggaatga tagctcaata tctggcaatt gattatcctg agttagttga
     9121 gaaacttgtg ttggcagtta cttcttcaaa gcaaaatgat actatccaaa atgttatttg
     9181 tagttggatt gatatggcga agaagcagaa ttacaatgat ttaatgattg acacagcaaa
     9241 gaaatcctat tctgaaagat atttaaaaaa ataccaatta tttattcctt ttttaggaaa
     9301 agtaggtaaa ccaaaggatt ttaaacgatt tattattcaa gcgacctctt gcatagaaca
     9361 taatgccttt tccgaattaa ataaaataac atgtccaacc ttaattatag gaggtgctaa
     9421 tgataaaatt gttggaaata atgcttcttt tcatttagta gaaaaaatta aaaaaagcga
     9481 aatatttatc tatgagggat tgggacacgc tacatatgaa gaagcacagg attttaatga
     9541 aagagtttta gagtttttaa ataagtaaac acctttgtta aaaatataca tatgaagatt
     9601 ggaaatttaa tgttaaaaat agaaacatga aaatatgctt aactggtatt tttactattc
     9661 ataaccaatt tttgatacat tatctactat aaatacaaat atagcttcaa tgtgattata
     9721 tattgttgta ttggtaaagc acttatacaa acagaggaat tttgtaaatt catattatac
     9781 ccacatttgt taacttatga aaatataatc aaaattttta tgagcttttt atataaaaaa
     9841 tataatgttt gtatacaaaa atattaaaga cattttagta agttttgtat ataagcaaac
     9901 atgtattttt taatatagtt atctaaatat tattttatat acaataaaat atgactccta
     9961 aaa
//
LOCUS       DAYAOX010000133         9935 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.133, whole genome shotgun sequence.
ACCESSION   DAYAOX010000133 DAYAOX010000000
VERSION     DAYAOX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.133"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            58..1899
                     /locus_tag="ACGI57_002568"
     CDS             58..1899
                     /locus_tag="ACGI57_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFG9717638.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVED
                     IKIENKSARLIGKEEALKYKLDSVVSKKVDKIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSSRESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            2204..2911
                     /locus_tag="ACGI57_002569"
     CDS             2204..2911
                     /locus_tag="ACGI57_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9717639.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            2905..4215
                     /locus_tag="ACGI57_002570"
     CDS             2905..4215
                     /locus_tag="ACGI57_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9717640.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNVAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKSEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            4332..5015
                     /locus_tag="ACGI57_002571"
     CDS             4332..5015
                     /locus_tag="ACGI57_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717641.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDEPFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            5008..7395
                     /locus_tag="ACGI57_002572"
     CDS             5008..7395
                     /locus_tag="ACGI57_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9717642.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGKMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            7675..8817
                     /locus_tag="ACGI57_002573"
     CDS             7675..8817
                     /locus_tag="ACGI57_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HFG9717643.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            8836..9360
                     /locus_tag="ACGI57_002574"
     CDS             8836..9360
                     /locus_tag="ACGI57_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFG9717644.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            9460..>9935
                     /locus_tag="ACGI57_002575"
     CDS             9460..>9935
                     /locus_tag="ACGI57_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HFG9717645.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNK"
BASE COUNT         4110 a          923 c         1763 g         3139 t
ORIGIN      
        1 atgtaaaata taaattttat attaaagcta aaactaattt gaggaggcta atttatattg
       61 aagattagaa ggtatttaat tttggtaatt ataattctaa ttacaacaag tcagagtttt
      121 atttcttatg ggctagagcg atttgaagaa ataaccattt ttcacactaa tgatatacat
      181 ggaagatatg ctgaaggtga tgaccatata caaataggaa atctagccac attgaaaaaa
      241 gaaactccaa actcaatttt agtagatgca ggagattgtt tgcatggact tccaatagta
      301 aatatggata gagggaaaag tgcaattgaa ttaattaaat gtgctggata tgactatatt
      361 actcctggaa atcatgactt taattatgga aaagatagac tgtttgagtt atctaaaagt
      421 gctaatttag gagagaatac tttaaaattt ttatcatcaa atatttttga aaatgggaaa
      481 aaagtttttg atagtaatga tataaaagaa atagatggaa taaaaatagg attttttgga
      541 ctctcaaccc aggaaacaaa gagcaaaact ggatttaaaa atgtggaagg attagagttt
      601 aaaaatccta tagaaagtgc aacagaacaa gtaaatgaat taaaaggaaa gggtgccaat
      661 gtaatagttg caatatgtca tattggtaca aatagtttaa gtaagccaaa tagtatagat
      721 atagcaaatg aggttcaagg aattgatttg attattgatg gacacagcca cactaagttt
      781 gaaaatggta aacaagtggg tgatacattg attgtaagtg caggacaata tttagaaaat
      841 attggcgaga taaaactaac tttggatact aatgatgtag aagatataaa aatagaaaat
      901 aaatcagcta gattaattgg aaaagaagaa gcattaaagt ataagcttga ctccgttgta
      961 agtaaaaagg ttgataaaat aaaagaacaa caagaaaaaa tattagataa agttataggg
     1021 gtaactacaa atactctaga tggttcatat gaaaatgtaa ggacaaaaga aactaatttg
     1081 ggtaatttaa taagtgacat tttactagat aaaactaagg ctgatatttc tttatttaat
     1141 ggagggaata ttcgagatac gatagaaaaa ggcaatataa caagaagaga tattgtagat
     1201 gtatttcctt ttagcaatac tatagttacc aaagaactta caggagcaca gattaaagat
     1261 gtattggagc atggagtaaa actttatcca gaaaaaagtt cagccttttt gcaagtggga
     1321 ggaatttcat attactttaa tcctaaacaa aaagaaggcg aaaaaataac aaatattcaa
     1381 aaagaaggaa agccattaga tttaaataaa aagtatgtag ttgcaacgaa tgattatata
     1441 gcatcaggtg gagatgaatt tccttgtttt tcaaaagagc cagtactaaa ggaatttgga
     1501 aatttagaga gtgcagtcat tgaatatata gagtacaaaa aagaaatatc aaaaaatgta
     1561 gatggaagaa ttggtattaa atctaaagat gagttaataa gcaagaatca tgagagtgat
     1621 ttgatattaa gtgaaaatga aaaaaatatc aatgataatg aagagaatct atctaataat
     1681 ataaaaagct ctgatagtga taaaaataaa agcttagata aaacaagttc tagagaaagt
     1741 tctaatctag gaatatctaa aagagaagct aaagaaaaat ctcctaaaac aggagattta
     1801 ggtttttcaa atagtataat tatatttata gtttcaagta cattgatatg tttattgaat
     1861 tttaatcaaa aagagttaaa ggataaaaag tcaaaataaa aattaatgag caaaaattag
     1921 tagatagtaa tttaatttta gataatagta aacagcaaag gaaattagtt atataaatac
     1981 taatttcctt tgttttatgt tttaacatga ttacttttaa ataaaattgt attttatgaa
     2041 aaataattat actaaatggc atagtattat ggggattttt agtttttatt caagtaaatg
     2101 aaatcatatt aacaacttct gatatattta caaattgata aaaaaatgat tttattagta
     2161 tgatataatt tagctagtat gtatgataat ggaggatata gggatgaata atatattact
     2221 tttggaagat gacaaatctt taaatagagg aattagtttt aaattaaaaa aagaaggata
     2281 caatgttttt agtgcatttg gtatagaaga agcaaaaaat attttcgcaa aagaagagat
     2341 atgcttaatt atttccgata taggtctacc tgatggtagt ggttttgact tttgtgagga
     2401 gattagaaag aaaagtgatg tttacataat aatgttgaca gctcttgatg aagaggtgga
     2461 tattgtcaca ggatatgatt taggtgcaga tgattatatc acaaagcctt ttagcttgat
     2521 ggtattaata tcaaaagtga atgcgcttat gaaaagagta aatacagtaa aaaattatac
     2581 tctattagtt tgtgatgatt tattctttta ttatatagaa aataaattaa ttataagaac
     2641 agatgataag gaagaagaga taattttaag taagacagag acaaaactat taaaatattt
     2701 gatggaaaat tctatgcaga cattgactaa agagcaactg ttagaatctt tatgggatag
     2761 tagtggtaat tttgtagatg ataatacgat tgcagtaaat ataagaagac ttagacaaaa
     2821 ggttgaaaaa aatccatctg aacctaaata tataaaaaca gtaagagggg ttggatatat
     2881 gtggggagaa aggagtataa aaaaatgcta aaaaatatga acctcaaaga tggtttaagt
     2941 gtgaaagatg attatataat gagaagagtt attgtatatt ctgttttatc tgtactaata
     3001 gtaacccttg tatttatact ttttgaatat agcagattac ataaattgta tgaacatcaa
     3061 ataaaaaata ctcaaaatgt tgctggaata ttgattgaaa aatatcctaa tgatgaaata
     3121 gatatagtaa attctattta taattctgat tatagtaagg tgtctactgg agaaaaggca
     3181 tttaagaaat ttggctatgg attggaaaat aaaatgtcaa atgataaaaa ttttagctta
     3241 tacttaaaat ctttttttaa ggaatcattt tttatttttt taatgatgtt tatagtagtt
     3301 ataggtataa tatattattt tataagatac ataaataaaa gattgagtaa aatttatttt
     3361 atagtagata agatgtcaca agaaaactac ttgaaggaca ctactgagtc agaagaaact
     3421 ggttatctag tagaatataa tgaatacttt aaagaaggca tatttagtaa gataaataac
     3481 tgtctatatg agcttaatag aagtttaaag ataaagttta ttaaattaga aaaagaaaaa
     3541 gaaagtgtaa aatcattagt aacagatatt tcccatcaat taaaaactcc tttagcatcc
     3601 ttaaaattat acaatacatt acttatagaa gaagaattag aagaagatga agccaacgag
     3661 ttcttattaa ccaataaggc ttctataaat aaattagaaa atttaataaa ttctttagtg
     3721 aacatatcaa ggttagaaat tagcatgata aacataaaaa aagaagataa tgatataaaa
     3781 aatactattc taaatgcagt aaaaagtgtt acccctaagg ctaagagcaa aagtataaca
     3841 ttaaatttaa atgaatttaa cagtagaatt ataccacatg ataaaaagtg gactgaggag
     3901 agtattttta atgtattaga caatgctgta aagtatacac aggtaaatgg agaaataaat
     3961 atatctgtag aagaaactgc taatttcttt aaaattataa tagaggataa tggcataggg
     4021 attgaaaaaa gtgagtttaa taatatattt aaacgttttt atagaggttc tgctgatgaa
     4081 attgaatctg tagaaggtag tggggtagga ttgtatttaa gtagaaagat tttagaagaa
     4141 caaggtggaa atattatagt ttcttcaaaa gtaggaatag ggagcaagtt ttctttgttt
     4201 cttacaacta tgtaatatta aatgaaagga aaatgtaagc ctctacattt attatataag
     4261 tataaacagc tccagaaata aaaaattaat aaaatatgga gtaatataac ttagaatgag
     4321 aggataattt tatgaaaata ctatatactg aaaatttatc aaagcattat ggaaaaggag
     4381 aaagcttagt aagagctttg gacaatgttg acttagaaat aaatgaaggt gaatttgttg
     4441 caataatagg aaaatcaggt tctggaaaaa gtacactttt gcatatgatt ggaggtcttg
     4501 atatacctac atctgggaag gtgtatatag ataataaaaa tatatttaca ttaaaagaag
     4561 aagaacttgc tgtatttaga agaagaaaaa tagggtttat atttcaaagc tataatttaa
     4621 ttccatctct taatgtatgg gaaaatgtag tgttgccaat cggtttagat ggtagagagg
     4681 tagatgaacc ttttataaaa gagcttttaa aatcattagg acttgaaaat aaacacgacg
     4741 ttttaccaaa tacattatca ggtggtcaac aacaaagggt ggcaatagca agagcattag
     4801 ctacaagacc agctataata cttgctgatg aaccaactgg aaacctagat tctaaaacat
     4861 ctgatgaggt tatgagtata ttaaaatcta tgtctaagaa gtacagtcaa actctagtta
     4921 tgataactca tgatgacagt atagcccaaa tggctgatag agttatattc atagaagatg
     4981 gcagggtgtc aaaggttggt gataaaaatg attaatacta ctaaaatagc aaaaagtaat
     5041 ttaaagcaaa ataaatcaaa aagtattcta ataataatta ctataatact ttcaacctta
     5101 atgctttctt ctattggaat ttatattgtt gatgcaggag catatcaaaa agaaaatact
     5161 ataaagtatt caggtaatta tcaaggtata ttagcaaatg tagacgaaaa acaagctgat
     5221 atattgagta atcatgcaga tgttgaatta actggtaaaa tgaatggagt aggtgttgaa
     5281 aaactggaag atgattcaaa tatttctcta gcatacatga atgaagatgc tttgaaatta
     5341 aatagttttg aatttatcaa aggaaaattg ccatcaaaag agaatgaaat tgtgttagat
     5401 agtggagctt taaaggcact tggttataaa aataaagatt taggtgaaaa gataaaaata
     5461 tcttataatg actataaaag tgataaaaaa attgaaaaag agttcattat aagtggaatc
     5521 ttaaaaacta gcgaaatatc tgaagcaggt aaatattatt atgctattat atcagaatca
     5581 tatatgagaa atactagaga tatgtcacaa gaagatttta atatatatgt gaaattaaat
     5641 gataaaagta atttatcgat agaacaagca aaatcaaaat taaataagat tgctaatgat
     5701 gtaggattgg acactattaa tgtagctgta aatgaaaact atataaatgc tttaaaacca
     5761 gatatggaaa caattatggg tggagtattt gtagggttag taattgtatt gtctagtatt
     5821 ttagtaatat acaatatatt ttatatttca atagttacaa aggttcaaga atttggaaag
     5881 cttagagcaa ttggtgcaac taaaaaacaa gtaaaaaata tagtatttaa ggaagggttc
     5941 atattagccg gaatagcaat accaatagga ataattttag gctatgtact tgcaaatatt
     6001 ataataaaat catttatgga tatagatgta aaatcatcac gattaccagt tatattatcg
     6061 gttgtggtaa taagctttat atctgttgtt ttatcacttt taaaaccaat gaaagtagca
     6121 tcaaaagtat ccattgttga cgcagtaaga tatacaggaa ataaaataag taataagagt
     6181 aagagaaaag gatataaaaa tataaatcta aagagattat cacatgccaa tttagaaaga
     6241 aataaaaaac gtacttacat gacattggca tcacttatac ttagtggaac tatatttata
     6301 acagtatcaa ctgccttaga aagttttgat gctgagaaaa tggcaagaga acactttcct
     6361 tatgatatag aagttagact atctggttat gaaatgaaca gtgataaaaa tcctaaagat
     6421 aatttgaata ttttacaaat ggataatcct ctaagtaaag atttttttaa tcaaataaag
     6481 aacatggaag gaattaaaag aatagaatct gcaagaagtg taaaaattgg tatggaggac
     6541 tatgatgttg aatttaagta tgacctttta caaagtataa atgaaaatga tgtaaaatca
     6601 ttaaacaaga atataataga tggaaaaata aatcttgaaa gacttcaaac tggagatgaa
     6661 atagtaatta ctcatgttga tacagcaaaa gaaatgggag taaaagcagg agataaaata
     6721 agattaactt tatatgatgg agataaaaag ataaaaaaag aatttaaagt tcaagcaata
     6781 gctatggggg ttccaagttt tggtataggg aaggatttta ttgatagaac tttgaaatac
     6841 gattcaacta gtagcttggg aatatataca gaagaaggaa agtatcaaga aatcaaagat
     6901 agtatcaaga agattgctaa aaataatgga tttttagaga ctgattttat agatagtaga
     6961 attgagtcaa ataaggctac tatttcattt ataaagataa tgggatacac gctaacaggc
     7021 ataataggtg tgattggatt tatgaatctt gtcaacacaa tgataactag catagttact
     7081 agaaaaaaag aacttggtat gcttcaagct attggtctta ctaataaaca attagtgaaa
     7141 atgcttaatt cagaggcaat gtattacact tctggaatga tgataggaag tattctattt
     7201 ggaggtatat tagggtatat tgctgttata tttctaaaaa agactggatt atcttatgca
     7261 acatattctt tgccaatagt accaatcttg ttgatgattg tgtgtattct tatagctcaa
     7321 tttataacaa cttatttgat aggacgaagt tttaataaag aatctcttat tgatagagta
     7381 agatacagtg aataaaatac aaatatttat ggttagttta actagcaaaa aacatataaa
     7441 aaaatatagt taaagagatt cataattaaa taggttaaag catagtacag agcaaacaat
     7501 ttaagcagat gtttgctctg tattttatta tgtaatcaat aatatatcta atatactgat
     7561 attattcatg ttagaaaatg taccggtaca attctaaaaa agtataaaat tgtaccatat
     7621 aaaatgaaaa ttttgtataa taatcgtata gaaaataaat aggagctgat atatatgaat
     7681 aagaagatta attatgcacc aggaccaact gaaactaggg aaaatgtaag attagcaaga
     7741 gcagaaaaaa caacaaatcc agatatagat attgattttg ttgaatttta taaaaagaca
     7801 tgcgagaagt ttggtaatat aattggaaca aaaaacgatg tttacatatt gagtggtgaa
     7861 ggaatattag gtcttgaagc agcatgtgca tcacttacag aaaaaggaga tagagtactt
     7921 gtaattgaca atggtatata tggagaaggt tttaaagatt ttgtaacaat gtatggtggg
     7981 gagtatgtgc ttttttcaag tgaatatact aaaagtatag atattgatga attgaaagca
     8041 tttttagatg aggatagtaa ttttaaatat gcaacagtag tgcattgtga tactccaact
     8101 ggcgtattaa atgatgtaag caaaatatgt ccattactta aagaatatgg aatattaact
     8161 gtagttgatt ctgtagcagg tatggtagga gaaagattaa gtgtagatga atctaaaata
     8221 gatataatat taggtggttc acaaaaagct atttctgcac cagcaggact tactatagtc
     8281 ggaataagtc aagatgcaaa aaattgtata aaaaatagaa aaactgatgt aataggtttt
     8341 tattgtaatt taaatatatg ggaaggctat tatgaaaaaa aatacttccc ttatacaatg
     8401 ccaataagtg acataatggg gcttgataaa gcacttgata atatattaga agaaggaata
     8461 gaaaatgtat taaatagaca tgaaaagata gcttatagtg ttagaaaatc tattgaggaa
     8521 tatggtctag aactattttt agaagaagga tattctaata cagtcacagc tataaaaatt
     8581 cctgaaagta taggagcttt agaacttact gattatatgc ttaaaaatta taatacactt
     8641 gttgcaacat cactaaatca atatatggat actattttaa gaataggtca tatgggagag
     8701 aatgcaaatc taaataaaat agaacatgta ctaaatgttt tggataaaag tttaagtgca
     8761 ttaggattca aaggaaatgg aaatttgtta aatttattta ataagtatta tttttaatag
     8821 atagggggat atagtatgga agttacagct aagcaaaaat ttacaacgaa agacatggtg
     8881 gaaacctcac tattaatagc attagtattt attgcaacta aatttataaa cataagatta
     8941 ccaatatcta taaatggtgg gcttgtgcat ttaggaactg caatgttgtt catatcagca
     9001 atagtatttg gaagtaaaaa aggtgcttta tcaggagcta ttggtatgtc actttttgat
     9061 ttgatatctg ggtggacttt atgggcaccg tttaccttta tagttagagg agttatgggt
     9121 tatttacttg gaaagattgc atgggcaaat ggtaataatg ggaataattt tttaatcaat
     9181 gtaataggaa tatgtgtttc atcaatttgg atgctatttg gatattatgt aacagaagtt
     9241 atactttatg gtaattttat agtacctctt acttcaatac caggtaattt aatgcaagta
     9301 ttgataggtc ttataatagc gcttcctatt tctaaggtat tgaaaaaatg tataaaataa
     9361 atataaagtg aactaaaatt tatgatagca taaattatac tgtataatat taggatggag
     9421 tacataatta aaaaatttta gtatagaggt tagttcacaa tgttatcagt tacaagatat
     9481 tcaatcaaaa atgttcgttt aaagccgtgg gtaaagttta tttggtattt tgagacaaaa
     9541 tcaaatatac tgttaaataa taagttactt ccgacagaca gtatagatat tattctcaat
     9601 ctttctgatg ttatggaata taaaattgaa aatcaagact atattgctag taatattcat
     9661 tttaatggaa taagggataa acatggattt attattcaac atggaaatat acgtgttata
     9721 gggatttctt tttatccatt tggtctctat ccatttttaa agataccaat ttcagagttt
     9781 aacagacaaa ttgttgacct agaagcagta tcaaaactat ttgcaaaaaa attagaagag
     9841 tcattaaatc caacaaaagc aatagaaaaa gttgttttat gtttagaaga agtattatta
     9901 tccatattag aagaagattt aatttcaaat aaata
//
LOCUS       DAYAOX010000134         9836 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.134, whole genome shotgun sequence.
ACCESSION   DAYAOX010000134 DAYAOX010000000
VERSION     DAYAOX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.134"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(77..1474)
                     /gene="asnS"
                     /locus_tag="ACGI57_002576"
     CDS             complement(77..1474)
                     /gene="asnS"
                     /locus_tag="ACGI57_002576"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFG9717646.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(1551..1784)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1953..2147)
                     /locus_tag="ACGI57_002577"
     CDS             complement(1953..2147)
                     /locus_tag="ACGI57_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HFG9717647.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(2420..4093)
                     /gene="cspC"
                     /locus_tag="ACGI57_002578"
     CDS             complement(2420..4093)
                     /gene="cspC"
                     /locus_tag="ACGI57_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid germinant receptor pseudoprotease
                     CspC"
                     /protein_id="HFG9717648.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLEPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(4103..7501)
                     /gene="cspBA"
                     /locus_tag="ACGI57_002579"
     CDS             complement(4103..7501)
                     /gene="cspBA"
                     /locus_tag="ACGI57_002579"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional germination protease/germinant
                     receptor pseudoprotease CspBA"
                     /protein_id="HFG9717649.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(7668..8864)
                     /locus_tag="ACGI57_002580"
     CDS             complement(7668..8864)
                     /locus_tag="ACGI57_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFG9717650.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSISYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(8885..9706)
                     /locus_tag="ACGI57_002581"
     CDS             complement(8885..9706)
                     /locus_tag="ACGI57_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9717651.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         3062 a         1889 c         1094 g         3791 t
ORIGIN      
        1 tctctaaaat agataaatat gcaagtcatt ttagtgttta tacttaaagt tatataagtt
       61 ttattatata taattattaa aattcagcat ttttaggagt tcttgggaaa ggtataacgt
      121 ctcttatgtt agacatacct gtcatataca taattattct ttcaaatcca agaccaaatc
      181 cagagtgagt agcagtacca aactttctaa gttctaaata ccaccaataa tcttcttctc
      241 ttaatcccat ttcattaatt ctatctaaca atacatcttc tctttcttct ctttgagaac
      301 ctcctacgat ttctccaact ccaggaacta gtaaatccat tgctctaaca gttttaccat
      361 cttcattcat tctcatatag aaagctttta tatcttttgg ataatctgta acaaacactg
      421 gacaattata tatttgctcc gttatatatc tttcatgctc tgtttgaagg tcacatcccc
      481 actcaacagg atactcaaat tcatgcccag attcaattaa taattccact gctttagtat
      541 acgttattct aataaactca gagtttacta tcttatttaa tctttctaac aatcctttat
      601 ctataaaact attaaaaaat tccatttctt caggagcatt ttctaataca tagcttataa
      661 catattttat catatcttct gcaagttcca tgttatcttc aaggtctgca aaaactattt
      721 ctggctctat catccaaaat tctgaagcat gtcttcctgt ataagagttt tcagctctaa
      781 atgtaggtcc aaaagtataa acgtttctaa aagaaagtgc cattatctcg gcatttaatt
      841 gaccactaac tgttaaatta gcctcttttc caaagaaatc ttgagtatag tctattttgc
      901 catcttcagt ttttggaata tcatttaaat ccatagttgt tattctaaac atctctcctg
      961 caccttcaca gtcactacct gttatgattg gagaatgtgc atatacaaag tttctttctt
     1021 ggaagaattt gtgtattgca taggcagcta ttgatcttac tctaaataca gctgaaaaag
     1081 tattacttct tggtcttaaa tgtgctatcg ttcttaaata ttcaagcgta tgtctcttct
     1141 tttgtagtgg atacgaacta tctgattccc cttctacaac tatgcttttt gcatgtattt
     1201 cgactggttg tttagcccct tctgtagata ctaactctcc ctctacaagt atagatgagc
     1261 ttattgctaa ttttcctacc tctttaaaat tttctacttt ctcatcaaat actatctgca
     1321 tatttttaaa gaagcttcca tcatttagct ctataaatcc aaaattttta gatactctag
     1381 atgttcttat ccatccagct acttttacag tcttccctat gtactgttct ttttctctgt
     1441 acaattcttt tactgttata aattcctttt gcattttaaa tcctcctagt ataattgtta
     1501 ttaatttctt ttaaatataa tttcaataaa aaaagccctt catccctata taaagggacg
     1561 aaagactatt actctcgcgg taccacccta gttgtcataa tgacctctta gttaagcaat
     1621 gcttaaagcc tataacggtg ccttccgtct aagtctactt actaaataag ctttcgatta
     1681 gaaactccaa gatgttcttc aatataaacc tcgtattgag ctttcaccat cctcaactcg
     1741 ctagaactac attttatatc tactcttctc ttcacagtat ttatctttta ttatttctgc
     1801 taattaacaa aattatacca atttataaat ttttagtcaa taaatttata taattgatac
     1861 tgattgttat ttaaaatata ataattctac cttacataat gataataaat acttatcttc
     1921 attacttctt tattacttca atttccaaaa ttttataatt tacttccatt gtcaacccag
     1981 tcttttgctt cttcgacacc cttaattgaa ggtattgaaa ctttactatg aggttttaat
     2041 ttttcaacat cagcccatgc tgctgtcgac tcatttccaa ctaaaagtga acttttaagt
     2101 cttttattat ctttaccttt taatgctctt gcctttttac tttccaaaac tatcacctct
     2161 attatttatt ttgtaatagt tttagcaatt ttacaaataa aattcataag tctaaattat
     2221 aattattttt aaattaacat aatatctttt caatacttaa tttataccta tttatataca
     2281 aaatatttac tttgtgtatt caaaatataa aactgttttt taattttcat atttattttt
     2341 tatgctattt ttctactcta tctaaagtat ttgatacaat taaactaata atcaattata
     2401 attttacata ggttcttatc tatagagtat ttgctatctg ttgaatcgta ttttttaaat
     2461 ttaaaatccc atatccctgc gatacatttg gatatgtata tatctcaagt tttgtagcac
     2521 ccaatattaa atatgttttt aaaacttgtg taaacaatga taatctcggt acattatcct
     2581 gtttttctaa gtattccatc aataaggcta aaacacctgt gactatagaa ctgctcactc
     2641 ctgttcctgt tcctgtatta tatgttccat ttttatatgt ggatattata tctacacctg
     2701 aagctactat atctggctta attcctcttc ctctaattgg tcctttcgaa gaaccaatcc
     2761 acatactatc agtcttattg ttaaatgtac caactgtaat tacatcatca ctagcagcat
     2821 acatagttat agtagctact gaatcaggct ctaaaaacct tgtgtctgga gctatcaaat
     2881 ttttatttgg caagtatatg tcatattctc catttattat aagttctgaa gtcaatctta
     2941 aaatccatac tccaggtttt atatctctta atcttatctc caaattttct tttccagagg
     3001 taatatatgg ataaataaat ctcattgcat aagtagtatt ttctaagtta aattttcctc
     3061 tatatatata aaagtctgga gaatacctta tatcatgact aacttcacct gaaggagata
     3121 ttatctgtgc tcccacttta tctggaccat tcgtatttag tgttatatca agagcatagt
     3181 catctccatc ttgtattatt acatcttgaa cttctcccac actgctaaat ctgccagaat
     3241 aatgaatatc tgtattccct tgatttcctg caccactcac tactaccaca ccagcactac
     3301 tcaatatatt aaaagtatct agtattgaag tagttgctac agcacttgac ttaactccaa
     3361 tagtcaaatt tattatcagt ggtttatttt ctgttcttgc tatgttagta acatacgtta
     3421 tagctgctaa aaaatctgaa acactgtaat ttatccttcc agcataataa gtatctgtat
     3481 atgattttaa tttaactact attaagtctg attctgtagt aatacctctg tactgagaat
     3541 ttatccttcc atttccaact aatataccag aaactaaagt tccagtacct atattatctt
     3601 gactcaaact cccatcattt ctatttatgg ctatatttaa ctcacttctt gtaaattcac
     3661 ttccaaaaat aaaaccttct ggaggtggat ttgtatttgc ttcttggtcc cataaatata
     3721 aaactttact agttccatca tcatttataa aatcaggatg caaataatct atcccagaat
     3781 ctataactgc taataaaatt cctctccctg ttatatcatt atatggattt tcataaatat
     3841 aatcagtttc agctgctgtt gtaatggttt ctccattatt tacattatta gttatttcaa
     3901 ttagactgct cattggttca gattcttccc accaagcaac ttgtattata ttatttaaaa
     3961 tagtttcatc aaaatctaaa ggtacatata ttactgcaag ctgactattt aaaaccatat
     4021 acctatttat tccattttct tgtagggcag attctatatc accttgataa attatacaat
     4081 aagatttttc cataaatccc tcctatctta aaacatcaaa cattcctcta acatccaaga
     4141 gcccatatcc agaatttgta tttggatata ctgtgtttga atcttttcta gcaccagctt
     4201 gcataaaagt ttttattttt tgcacatatg cttggtttgg atatctaccg tcaacaaaag
     4261 tatattgaaa atacattgca gcagcaccag ctgcatgtgc acttgctgct gctgtaccag
     4321 ttatagttgc atacgtgttc ccaggataag cagctattat attgacacct ggtgctacta
     4381 tatctggttt taacctgtct tcaatagttg gccctcttga tgaactttgc cataaactac
     4441 cattaatggt attgtaggct cctatggtta ttaaatcatc ttgaatcgca ggataattta
     4501 tagtgtaaaa agggtctacc tctctaaatc tagttccact ttttaaaagt tctctatttg
     4561 gaaggtataa gttatatctt cctgttataa tataaactcc tactaatcga atcttccata
     4621 cacctctttt tgcattcttt aaagtaacat tagtaaattg ctgtccagaa aaagttgttg
     4681 ggtatatata tgttatagaa tattcagtcc cctctaaatc aaataaacct gttactttat
     4741 tataatttga aattccaaca cttttacttt cttcaccagt gggagatact attattacat
     4801 cagctttatc tggtctattt aaccaaagtt ctaatgataa ttcttcctcg tcttcattta
     4861 actctaattc aacttcaaca gaacctccaa catatggtat tattccagat gtatgtgtct
     4921 gtgtattgcc ctcatttcct gctcctgccg ttatacataa ccctctagta aagaacgctt
     4981 tttcactatt actcctattg gttaaacctg ctaggctact ggtacctaaa gacatattta
     5041 taactaatgg tcttccaagt tcaaaagctt ttttatatgc atactgagat gcagcaaaca
     5101 acattgcact attataaaaa tcatctatct tgccgagttt tattattatt agctcagaat
     5161 cttctgctat acctgcatat tcactattta catttccaag ccctgcacat atcccactca
     5221 acatagttcc ttgaccaact tcatcctgag atagactagg gtcattttct gcgatagctc
     5281 gatttatatc ttctcgtgta tattctgtcc caatataaaa accatcagga ggagttcctt
     5341 cttttgtttg gtcccataaa tatactattt ttgaagtgcc atctggataa ataaaatctg
     5401 ggtgtagata gtctattcct gtatctgcaa ttgatattag agtccctctt cctgttatat
     5461 taatattagg attattttta aagaaattta cgcccatttc ttctgttgca actacacctc
     5521 caaaagtacc tcgattaatt tctccaagta atgtcatctt tgtagtcgat acagttctta
     5581 tgattgaagg aagttgcaaa actcgttgta tgctattata gtctgtatta tttatagata
     5641 gtacacctaa tgaatcactt atcttaaaaa acctttgact catacctaac ccagctagtt
     5701 cttcttcaaa ttcaggagta tggactatat ctattcctga tagtattcct gattgtctac
     5761 ttgcattttt acttatataa atttcattct ctaagtcatt atgtatttgc atattataac
     5821 catctataaa cttataacta ttttcctcat ctttaaatga cttattaata cttctatatc
     5881 caatatcctc tgtttctaaa tcttgattta tattagatag agtgtacaaa tctatatctg
     5941 ataaatttaa aaaaccaaat cctgatgaat tatttggata agattgatta cttaatctcc
     6001 ttgctccctt tagaagtaat gcccttaatt tctgtgaata caaaaataaa tcatttccat
     6061 ttacaattcc ccattccata aacaatgaac aaacccctgt aacatgaggt gtagccatgc
     6121 tagttccagt taatgctcca cttgtacctc ctggtaaaaa agatactata tcttcacctg
     6181 gagctaataa atcaggttta aaaacaccta attgtgtatc tccttcacca gaaaatatag
     6241 atactatatc tgttcttgaa ttaaaactac ctactgttat aactctactt gctgttccag
     6301 gaactgttac agtaagctct tgagtaggaa tcagaaatct agtatttcta tttagacctt
     6361 cagaagttgg caaatatata tttacattac cagttactat atctattggt tcaaatacaa
     6421 ttttccataa cccaggagtt atctgtgtat ttgaacttaa ttgtagagta actcttcttg
     6481 tgagagaata cggtgctata gggtaaaaat aaccagttat tctagtttca cccaaagtat
     6541 tccttatctc acctgaagtt agagagattg cttgtgtttg attatttgat gggtttacca
     6601 aatgtacact aaaatcatca acaaaatctg gccatatatt tatgtttaat atacgctctc
     6661 cttccccaac tataaactca acttcttctg taatattatt ttgaagccta atccttttat
     6721 gacctccttt atctgcatta tttccagctg ctacaactat attgtttttc caaaataaac
     6781 acatatcatc tatatattgt tcaaataaag atgttcctct atgtgaacct tcattacttc
     6841 cataacttat atttaatgta acaggcattc ttaattctaa tgccctatct aaaataaatt
     6901 taattgccct cataaactct gtacttctcg aaaaaatatc ggtctgtata tttccaactc
     6961 taaccactat tattctggca tcacttgcaa tagttgcaca aatacctgca acatgtgttc
     7021 catgtaagct agttgttgaa ataggtatat acatacttcc atctattgca ttatttatat
     7081 cttcattagt atataatgtt ccttctctaa aaccttctgg tggatttcct tgaatagatt
     7141 ggtcccaata atataatatc ttagaccttc catcactatc tctaaacaca ggtagagtat
     7201 aatctattcc cgaatcaatt ataccaatta tagttccttt tccagttaaa ccagttctat
     7261 ttttaaatcc tgttattcct gtacttgaaa aactttgcac atcctgagtc tgtaatataa
     7321 aaggcttttc tataaattca atttctggat aagttaaaag tatatttaca tcttcttcat
     7381 tacttgatgt tattattgca tatgatgaat taagtatctc tactgataca cctaattctt
     7441 cttctaacct caatatatct ccattgtatt ttacaattaa ttcataattt attataatca
     7501 cctcgacttc taaaattatt attaatgtat atgttttgtg tgagtctaaa atgtattaga
     7561 ctatataatt ttttaagttt cagttataca atttataata gatatattaa aaaaataaat
     7621 aatgatggta caattttgaa taaatcatac catcatttat atttacttta tcttgaaacc
     7681 attaaatttt tcaatgcttt ctcaagacct tttacagtca atggaaacat attaaattca
     7741 ctttgtatca ttctttgagt ttgataccaa taactaattg aatcccatcc atctctaagt
     7801 tctggattca tccatacaat tttactgtac tttctcttaa accttttaag ccactctata
     7861 ccaggtgtat aattatatgg accacgatag ttaccaccaa tatgaagcaa ctcagaaggc
     7921 gacatagaag catctccaac aattataact ttatagtttt tatctatatt ttttaatatc
     7981 cattcagtat taatagattt acttaaccaa cattcaggtg ttgtaaataa tctatcataa
     8041 atacagttat ggaaataata tatctttaca tctttaaaat gatttgattt acttacagac
     8101 tgaaataatg tattacataa agtgctatac cctctcattg aaccacctga atccataagc
     8161 aataaaagct tcactgtatt ttttcttggc ttctcaaaaa ataactttaa atatcctgca
     8221 ttattacaag tctcttcaat agtcttatcc aaatcaagct ctgtctttgg agcatctatt
     8281 ctagtagaaa attgacgaag tctctttagt gcaacttgaa attgacgcat atttaaaact
     8341 tcatcatctc taaaatctct gtattttctt tctcctgcaa cttcaagaac agctctacca
     8401 taagtagaat ttccacctat acgtatacca gtttgccctt ttcctgaatg tcctaactct
     8461 gaagtgcctc ctgtgcctat ccaataatta ccaccattat gaggagaatc ttgttctttt
     8521 attcgttcct ccaatttagc tcttaattta tcaagattta tatttggatt ttgttctaat
     8581 ctttcaaact cttctaaatc tatatctggt tttttaagcc aattcataat ctcttctgga
     8641 atttcttctt ggtgttctat acctttaaaa tactccaaaa acactgcatc aaacttatca
     8701 aaatcacttt cactttttat taaaatcatc ctgcaaagat agtaaaaatt tgaaaagttt
     8761 gaatagcaca gtcctttatc caatgcttca attaaagtca tccattcatt taaagataca
     8821 tttaatcctc ttgacctcaa taaataaaaa aattcaataa acataattat ccagctccta
     8881 ttaactataa gtgtctttgc atagattcaa tatcctcatt attcttaagt agtattcctg
     8941 caaaaggaac ttctttttct attttttcaa taggcattcc actcaaagtt aatgcttgta
     9001 tccagtctat taactctgat gtactaggct ttttttgtat gtcctttaaa cttctaatcc
     9061 aataaaaagt agtcattact tgttccaata ggtgttcttc tactttatca aaatgtactt
     9121 taactatttc ttccatcatg tctctatcag gaaattctat ataatgaaat atgcatcttc
     9181 ttaaaaaagc atctggaagt tctttttccg catttgaagt tataataact attggtcttt
     9241 gtttagctct tacagtttct tttgtctcat ttatgtaaaa ctccatttta tccaattccc
     9301 aaagcaagtc atttggaaat tctaaatccg ccttatctat ttcatctatt aaaagaatta
     9361 cttgttgatt tgagctaaag gcctctccta gtttaccata ttttatgtac ttgctaatat
     9421 catctacacc ttctcctcca aattggctat cataaagtct ttgaacagta tcatatacat
     9481 aaagcccttc ttgtgctttt gtagttgatt ttatattcca aattactaaa tcttttttta
     9541 attcatttga aattgcttgt gctagcatag ttttacctgt tccaggttcg cccttaatca
     9601 aaagtggctt ttcaagagca atagcaacat taactgatgc catcaattct ggtgatacta
     9661 cataatcact acttcctcta aagttagata tctctttttt tatcataaat cctactcctt
     9721 ttaaacagta ttttctaaat gtatatagac tttattacta tttttgatat aaaaataata
     9781 atacaataca tttgtatagt attattctaa aattctatat ctaaataaaa tcacct
//
LOCUS       DAYAOX010000135         9697 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.135, whole genome shotgun sequence.
ACCESSION   DAYAOX010000135 DAYAOX010000000
VERSION     DAYAOX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.135"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            193..657
                     /locus_tag="ACGI57_002582"
     CDS             193..657
                     /locus_tag="ACGI57_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HFG9717652.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTYETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            1294..2310
                     /gene="gap"
                     /locus_tag="ACGI57_002583"
     CDS             1294..2310
                     /gene="gap"
                     /locus_tag="ACGI57_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFG9717653.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            2705..3874
                     /locus_tag="ACGI57_002584"
     CDS             2705..3874
                     /locus_tag="ACGI57_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717654.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYSPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            4254..8507
                     /locus_tag="ACGI57_002585"
     CDS             4254..8507
                     /locus_tag="ACGI57_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HFG9717655.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            8656..9150
                     /locus_tag="ACGI57_002586"
     CDS             8656..9150
                     /locus_tag="ACGI57_002586"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFG9717656.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            9266..9643
                     /locus_tag="ACGI57_002587"
     CDS             9266..9643
                     /locus_tag="ACGI57_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HFG9717657.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         3920 a         1003 c         1751 g         3023 t
ORIGIN      
        1 ttttcataat atgttataaa ccaatttaag atactacaat aattaaaatt ccaaaattat
       61 ttaacatatt tgcaattaaa atgagaaaag gtaaaggttg aatgattgaa aaatttgatg
      121 taaaatagta gcttagttta atattatagt ttgtagggtt aaaaattaga aattgtaagg
      181 gagataaatt ctatgaaaaa attagttatt attttaatta gtatcattag tatggttagc
      241 ttgacagctt gtaaaaaaag caatgaaagt aaatataata atttaaacaa caaagaatcc
      301 ggacaaacaa ccattgaaat ggacttagat aaaaattatg atacttcaga cccatttgtt
      361 aatgcacgtt tgttttgtgt gtctaatgat atagatgttt tagacacaga gatttctttt
      421 aaaatggatg gagatagtgg tattgtggaa ataaaggaca ataaaacata tgaaacttta
      481 tggagtaata catggcatgg aagcgttgat aatgatacat tcacaatttc tctttctaat
      541 atacaaaaag agaaagagta tgctatatgc tttactggaa ttaaaataaa tcatgcagtt
      601 gtaaaggtta cttttgaaag caatttagtt aaggagaaag aaaaaccctc aaaataaatt
      661 ccagattgtc tataacttga tattcagtac ataaaagcag ttgatattta tgattgactg
      721 ctttttctta tcaaaaagga gagtttgtgc atttttactt gaaatagttt atgcacatca
      781 aaagttggtg agcatgatat tggaaatggt aagcatgaaa tcttgataag acaaggaaat
      841 aaatttaagt tttcaattaa gtatatttcc ataatttagt taacaataag ctaattatta
      901 tttatatttt gatatataaa taataattgt agcaaatttc aaaaaatcat atattttttg
      961 aactaaataa aaatttattt ttattatttt aaattagagg agattcttga agatatatta
     1021 tttttaaaaa taagtatagt ttaaaaattc aaaaaaaaga tggcaaaata tgtcccaagg
     1081 gacattgagt aaaataaatg atttcagctt atttacacta aaaagtacat actaaatgag
     1141 gggtaaaata aagaaaaatg atatactaat tgtcttttga taataaaaaa acatggaatt
     1201 tgattattta atcaagaaaa agtattgaac aaatgattta tttagtgtac aataatcatg
     1261 taaaaacata gtaatggagg gagacatttc ataatgaaaa ttaaggtagg aatgaacgga
     1321 tttggaagaa taggaagagc tgtattaaga atagctcaag aagaattagg agataatata
     1381 gaaattgtag ccataaatgc aagagcaaca acagaatctt tggcacattt atttacatat
     1441 gattcatgct atggaacttt tagaggtgaa gtagaggcaa aggatgaaga cactctagta
     1501 gtaaactcta aagagataaa aatattaaga tataatgacc cagaagaatt accatggaaa
     1561 gaactggggg ttgatatagt aatagaatca acaggattat ttacacaaag agaaaaggca
     1621 gaaaagcata ttaaagcagg agcaaaaaaa gtaattataa cagctccagg aaaaaatgaa
     1681 gatataacta tagttatcgg agttaatgag aaagaatatg ataatgaaaa gcacaatata
     1741 atttcaaatg catcttgtac tacaaattgt ctagctcctt ttgctaaagt attggacgaa
     1801 aaatttggta tagtaaaagg gttaatgact acagtacatt catatactaa cgatcaaaga
     1861 atattagata aaagccataa agatttgaga agagcaagag cagcggctga atctataata
     1921 cctaccacta caggagcagc aaaagctgtg tcaagagtac taccacaatt agaaggaaaa
     1981 ttaaatggtt tttcacttag agtaccaaca ccaacagttt cacttgttga tttagtgtgc
     2041 gaacttaaag aaaacgttac atctgagcaa gttaactctg tgttaaaaga tgctgctgaa
     2101 ggagaattaa aaggtgtttt aggatattgt gacaagccac ttgtttctat agattataaa
     2161 ggagattcaa gatcatctat tatagacgct ctatccacaa tggttataga agacaatatg
     2221 gttaaggtag tttcttggta tgataatgaa tggggatatt cttcaagaac tgttgactta
     2281 gtaaaatatg tagctgaaaa attaaaataa tatatatttc ttaaagcaaa ttttaaaaaa
     2341 attttttaaa aaaattatat aaaactaaaa aaaaagatta tgaaaaatat tgaaggaaaa
     2401 attttttcat aatctttttt ctttcatgca aaaagataca agttacaact tatatttgca
     2461 tattaggtcg ttagagaagt atatatatat ttagtaagga atcatacttt ttataaaatg
     2521 aaagataaga tgtcgaaaac ttgcataaat ctgaaaaatt tttattatta aaatactgat
     2581 ttccatttta aaaatgataa aaaaatgaca aataaagttg caaatagtaa tacttataag
     2641 tataattaaa aaagagaaaa ggttttgtta aactttataa caaaaaaaga agggatgatt
     2701 caaaatggag aagttaacag acaggagaca ggttaaggca gtaagcatgg aaaccttcat
     2761 tttcctaata ttactagtag ttggttttgg gtatgttggt agtattatgg gagcaggaat
     2821 gatgttcaaa gtaataatga gtacagcaca tgctttacta ttggagacag ttttcttaat
     2881 tatggctatg gcagttcttg caggcgcatt aagcgcacta ctttcagagt ttggagtaat
     2941 agcgttaatt aataaaattt tcgctgtatt tatgaagcct ttatatggac tacctggtgc
     3001 tagtatagca ggagctataa caacttattt atcagataat ccagctatta taccttttgc
     3061 taaagataaa acattcacac agtattttaa acaataccaa gttccagcat tatgtaactt
     3121 aggaacagcc tttggtatgg gacttatctt aacaacattt atgatttctc aaggtacaga
     3181 atatgtactt ccagcattaa ttggtaattt aggtgcaatt ataggtagtg tcataagtgt
     3241 tagaataatg ttgacattta ctaagaagtt ttataaatac agtccggaag aagataaagc
     3301 aacaggaaca ttagagaaaa aggatgaatt tagagaaatc agagaaggaa atgtatttca
     3361 aagagcttta gatgcaattc ttgaaggtgg taaaatgggg gtagatatgg gaatggctat
     3421 aattcctgga gtcctagtag tatgtacatt ggtgatgtta ttaacttttg gaccatctac
     3481 agaccctgtt acaggtcaag aagtttatac aggagctgca tatgaaggaa taaaattact
     3541 tccagttatt ggagataaac ttggatttat attagaacca ttatttggat ttacatcacc
     3601 agaggcaata gcattcccta taacttcatt gggagctgtt ggcgcagcaa tgtctctagt
     3661 tccagagttt ataaagtcag gagctataac tcctaatgat atagcagtat ttacagctat
     3721 gggtatgtgt tggagtggat atttaagtac acatattggt atgatggatg cattaaatgc
     3781 tagacagttg gcaggtaaag ctattctttc tcatacaata ggtggtttat gtgcaggagc
     3841 agctgctcac tttatattta cactcgtagg ataaatgtaa tcagctaaga taaaaaataa
     3901 tatacaaaaa atggttgttg atatgtaata agaatttaca tatcaacaac catttttttc
     3961 tgactaaaga taatttataa tacataaaca ttaagtggat attagagtta agttatattt
     4021 atgtaaaaag taacaaaaat ctgtagaaat ttatgacaaa atttagtaca ctaactatat
     4081 gttcttattt aaaatttaag gttagaatta ggaattacct agtagagtaa gctttgatta
     4141 ataaaagaat cataaaataa aaatattagt cataaagatt ataaataact tacttaaaat
     4201 aaagccatag aacttacaaa aattaaaaaa gcatataaat atggaggggt acaatggatg
     4261 aattatttca tataggaatt gatgtaggtt ctacaacggt gaaggttgtg gtacttaata
     4321 atagtaataa tatagttcat aaagaatata gaagacatta ttctgatgta aaaaaatcag
     4381 ttaaagaagt actaaatggt atatatgaaa aattgggaga tataaatact accattataa
     4441 taactggttc aggtggaatt ggaatatcaa aaaaattagg tgtaaaattt gttcaggaag
     4501 ttatatctag tacaaaatca atagagtatt ttcatcctga gactgatgtt gtcatagaac
     4561 ttgggggtga agatgcaaag ataacatatc taagtggagg aatagaccaa agaatgaatg
     4621 gaatatgtgc aggtggcact ggagcattca tagaccagat ggcatcactc ttaaagacag
     4681 atgccagtgg attaaatgaa cttgctaaag ggtataatgt tatatatccc atagcttcaa
     4741 gatgtggtgt ttttgctaaa acagatattc aacctcttat aaatgatgga gccaatcaga
     4801 ctgatatagc aatgagtatt ttcaatgcag ttgtcgtaca aactgtgagt gtcctttctt
     4861 gtggaagaaa aatagaagga aatgttgcgt ttttgggtgg tccattgtac tttttatctg
     4921 aacttagaga agcatttaaa agagtattag atttaaaaga tgaaaacata atcttcccag
     4981 aaaatgcaca actttatatt gctattggag caggattatt atcagtagat gaagataata
     5041 tacaactaaa atctctaata gaaaaattgg atagtataaa gaatattgaa gatggagaag
     5101 taaatttatt agaaccttta tttaaagata aaaatgagta tgaagaattt tcaagaagac
     5161 atgaaaaaga aaaaataaat tatgtggaca taaattcaat aaaaagcaat tgttatttag
     5221 gaattgatgc aggctcaact acaactaaag cagcacttat tgatgaggat ggaagacttg
     5281 tatattctta ttataatagc aatgaaggaa atccattaaa aacaaccatt aaagttataa
     5341 atgaagtata tgatatatta ccaaaaaata ttaaaatatt gagttcaact gtaactggat
     5401 atggagaagg tcttattaaa aaagctctta aaatcgataa tggagagata gagactatag
     5461 cacattacaa agctgctaaa tttttcaaca aagatgtaga ttttatactt gatattggtg
     5521 gtcaagatat gaagtgccta aaaataaaag atggagtaat tgataatata atattaaatg
     5581 aggcttgttc atcaggttgt ggctcatttt tagagacatt tgcaagttct ttatctatga
     5641 caatagaaga atttgcgcat gaaggtattt attctcaaaa tcctgtagat ttaggttcaa
     5701 gatgtactgt atttatgaat tctagagtaa aacaagctca aaaagaaggt gcaagtgttg
     5761 gagatatatc agcagggcta tcttattcag taataaaaaa tgctttattt aaagtaataa
     5821 agattagaga tttaaatgag attggtaata atattgttgt acaaggtgga accttttata
     5881 atgatttggt gcttagaagc tttgaaaaac taattggaaa aaatgtaatt agacctaata
     5941 tttcaggaat tatgggagct tttggttcag cacttatagc taaagaaaaa tatgaaagtg
     6001 gatacaaaac atccttgtta tctaaggaag agttgaataa tatcaagcta gaagcaagtg
     6061 taactagatg taaaggttgt tcaaatcatt gtcttcttac aataaataaa ttttctgata
     6121 atgaaatttt tatttcaggt aatagatgtg agaagggtga agctatttat ggtgataaaa
     6181 aaataggatt agaaaagaaa aaaccaataa atttatttaa gtacaaatac aatagaattt
     6241 ttagatacaa accattggaa aaagaagaag caaaaaatgg ggaaataggg ataccaagag
     6301 tattaaatat gtatgaagat tatccatttt ggtttacttt tttcaatgct atgggcttta
     6361 gagttgtact ttcagatagg tcatcaaaac agttatatga aactggtata actagtattg
     6421 catctgaaac agtttgttat ccaggaaaat tagttcatgg acatatagaa aatttaattg
     6481 caaaaggtat aaaaaatata ttttatccaa gtgtaacaaa tgaaaataaa gaggattcaa
     6541 atgcagataa ttattataat tgtcctgttg taatatcata ttctgaagta ataaaaaaca
     6601 atgtagagaa tataagaaat aagaacataa attatataaa tccatttata agtctaaatg
     6661 ataaggaaaa attgaagaaa agactctatg atgaattatc aagacatttt tctgatataa
     6721 aaataactaa agaagaagtt aatcatgcag ttgataaagc tactgatgaa cagaatttat
     6781 ttaaaatgga gatacaagca gcaggtgaaa gagcacttaa agaaataaaa gaaagaaata
     6841 tgaaggggat tgtgttatgt ggaagaccat atcatataga cccagaaata aatcatggaa
     6901 tgccagagct tataaattca ttagatatgg cagtgcttac tgaagactct atatgtcatt
     6961 tagccaatgt acaaagacca cttagagttg tagaccaatg ggtatatcat tcaagattgt
     7021 ataaagcagc tagttttgta agagataaaa actatttgga acttgttcaa cttaattcat
     7081 ttggctgtgg attagatgca gtaactacag accaggttca agaaatactt aatgaaaaat
     7141 cgaagatata tactattata aagattgacg aaggcaacaa tttaggagca gctaagataa
     7201 ggatgaggtc cttaaaagca gctatgtttg agagagaaag caaaaatata gatgtaaaaa
     7261 atataaaggt aaacaggata cagtatgcta aaaataatca gataacgaac aaacatacta
     7321 tattagcacc acaaatgtct ccaatacatt tccaattcct tgaaaaagct gttaacttaa
     7381 gtggttataa tatagaaatt ctaaaagata ctgatagtag tgtaattgaa gaagggttaa
     7441 gatatgtaaa taatgatgca tgttatccag ctataatagt tgtaggtcag ctaataacag
     7501 cattaaaatc tggcaaatat gatttaaata acacttctgt tactataact cagactggtg
     7561 gaggttgtag agctacaaat tatattggat tcttaagaaa ggctatatac gatgctggat
     7621 ttaaagatgt tcctgtcatt gcacttagtg taaatggaat agaagatagt ggtataatgg
     7681 ataatatatc actaaaatta atcaatagac tctttatgtc tgttgtttat ggtgatttgc
     7741 tgatgaaagt actatataga gttagaccat atgaaaaagt tactggaagt gcaaatgctc
     7801 tatatgaaaa atgggttgat atatgcaaat cttctcttgt taaagcaaaa atttctgtat
     7861 ttactagaaa tataaaagat atagtaaaag aatttgataa tttagagata ctagatatca
     7921 aaaaaccaaa agtaggtttg gttggagaaa tattggtgaa attccatcca attgcaaata
     7981 ataatttagt agatatatta gagcgagaag gtgctgaagc agtcgtgcca gaccttacaa
     8041 atttcttctt aagttgtgcc tttaatacta tatacaaaca cactcattta gaaggaagta
     8101 gaaagagtag aatgatagga gaggcattta tatacataac tggaatatat caaagagtat
     8161 ataaaaaagc acttgataag agtgagagat tttatgctcc agccaatata aaaaatgttg
     8221 ctaaaagtac agaaccagta gtgtcattag gtaatcaaac tggagaaggt tggcttttaa
     8281 caggtgaaat ggttgaatta ttaaatgaag gtgtagaaaa cattatttgt atgcaacctt
     8341 ttggatgttt acctaatcac ataattggta agggttctat aaaagaatta aagaggttgt
     8401 ataaaaatgc aaatataata cctattgatt atgacccttc tgcaagtgag gtaaatcaac
     8461 ttaacaggat aaaacttatg ctttctaagg cttttaaaaa tatataagat ttatattttg
     8521 atatgttaaa agttaatgaa tttaaaaata tactctatat taagtatcta taaaaaagag
     8581 ttatcctatt ttttagggta actctttatt agttaaaaaa ttatatgtat actgatatta
     8641 aaggggggaa gtaatatgag tttagtatat gatgtttata aatcaaaatt aggaaatttg
     8701 tacatattaa gtgaagatgg ttttatagtt agtatataca taggagatga aaaatttaat
     8761 aatttaaaaa atgaatgtat taaaaaagga attgatataa agagaagtaa ggaagattta
     8821 aaggaagcaa taagccagat agatgattac tttaatggaa aaagaaaagt ctttaattta
     8881 aatattcgta ttgaagggac tgaatttcaa aaatcagttt atagggaaat gttaaaaata
     8941 ccttatggag aaacattgtg ttattcagat atagcaagaa atataaacaa tccaaaatca
     9001 gtaagagcaa taggacaagc aagtaaaagt aataaaattc caataataat accttgtcat
     9061 agagtagttg gcaaaaaaga tattggtgga tatatgggga atcattctga tttgaaagag
     9121 atactattaa atttagaaag acagagttaa cttagaaaga aatgttttta ttgttaatat
     9181 ttgatataat aatagtaaaa agaagtattt tttacaaaaa caatgaagaa tttattttaa
     9241 tttacaaaaa tgagggggaa ttttaatgtg tgataaagtt atagcatcaa aatatcatga
     9301 gaagggattt aattgtgcag aatcagttat aaaagcatat aatgaggaat ttaatacaga
     9361 tataccagtt tgtttaggaa gtggactggg ttcagggtgt ggggttgcta gtttatgtgg
     9421 tgcagtaaat gcatcaaata taataattgg atatgtaaaa ggcagaaacc acgaagatga
     9481 aagtactaaa gctaaagtat atgctaaaga tttaactaca acagtaagaa aagagtatgg
     9541 ttcagagtta tgtatagacc taaaaaaaga tttagttgct tgtagagaaa taatggattt
     9601 cgcatatgat agtctaaagg aaactttaaa aaaagaatta taagaaaata agctatcaaa
     9661 tataaattat ttgatagctt atttttatag ccgatac
//
LOCUS       DAYAOX010000136         9686 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.136, whole genome shotgun sequence.
ACCESSION   DAYAOX010000136 DAYAOX010000000
VERSION     DAYAOX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.136"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..903
                     /locus_tag="ACGI57_002588"
     CDS             <1..903
                     /locus_tag="ACGI57_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9717658.1"
                     /translation="EGFNNVSTISNNIILYTWIITVVVYLIYTIFKYIKLKNLINDLS
                     YDVDDGEIVNLYKNILEEFNITKDIKLKYSYEVETPAFFNSCVLLPPHEYKLKELDWI
                     FRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHIDLDCELYCDERVLKNRNSE
                     EKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMNMFNEKYKNGILMALTLCLL
                     SSITFLKVNSTSIVNLNILPKGMEEKDVTNSAIVTMIEFTYAEAPKSMREEHKINCEF
                     LGLIPKDSDKIGVGIEYYDSLLND"
     gene            1819..2841
                     /locus_tag="ACGI57_002589"
     CDS             1819..2841
                     /locus_tag="ACGI57_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9717659.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            3137..4057
                     /gene="pyrB"
                     /locus_tag="ACGI57_002590"
     CDS             3137..4057
                     /gene="pyrB"
                     /locus_tag="ACGI57_002590"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFG9717660.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            4096..4788
                     /locus_tag="ACGI57_002591"
     CDS             4096..4788
                     /locus_tag="ACGI57_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFG9717661.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            4781..5683
                     /locus_tag="ACGI57_002592"
     CDS             4781..5683
                     /locus_tag="ACGI57_002592"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFG9717662.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETSSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            5768..6352
                     /gene="pyrE"
                     /locus_tag="ACGI57_002593"
     CDS             5768..6352
                     /gene="pyrE"
                     /locus_tag="ACGI57_002593"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFG9717663.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            6566..7492
                     /locus_tag="ACGI57_002594"
     CDS             6566..7492
                     /locus_tag="ACGI57_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HFG9717664.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(7544..8434)
                     /locus_tag="ACGI57_002595"
     CDS             complement(7544..8434)
                     /locus_tag="ACGI57_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9717665.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            8622..9653
                     /locus_tag="ACGI57_002596"
     CDS             8622..9653
                     /locus_tag="ACGI57_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFG9717666.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
BASE COUNT         3825 a         1006 c         1703 g         3152 t
ORIGIN      
        1 gagggattta ataatgtttc aactataagt aataatatta tattgtacac ttggattata
       61 actgtagtag tttatttaat ttacactata tttaaatata taaaacttaa aaatttaata
      121 aatgacttat cttatgatgt tgatgatgga gagattgtaa atctttataa aaatatatta
      181 gaagaattta atataacaaa agatataaaa ctcaaatact cttatgaagt tgaaacacca
      241 gcatttttta attcatgtgt gcttttgcca ccacatgagt ataaattgaa agagttagac
      301 tggattttta gacatgagct tatgcatttt aagagtaggg acctttattt aaaatatgta
      361 atcctgtttt taaaaacagt atattggttt aatccattta tatatataat ggataaacat
      421 atagatttag attgtgaact ttattgtgat gaaagagttt taaaaaatcg aaattcagag
      481 gaaaaaaagg attatgcttt aacaattcta aatgcaatga gaaaaggttc aaatacttca
      541 aataaattta tagctggact ccataagcaa tctgatatta aaaagagagt tatgaatatg
      601 tttaatgaaa agtacaagaa tggtatatta atggcattaa ctttatgttt attatcttct
      661 attacttttt tgaaggttaa ttcaacgagt attgttaatc ttaatatatt gccaaaagga
      721 atggaagaaa aagatgttac taatagtgca attgttacaa tgatagaatt tacatatgca
      781 gaagctccta aaagtatgag agaagaacat aaaataaatt gtgaattctt aggtttaata
      841 cctaaagact ctgataaaat aggtgttggt atagaatatt atgacagtct attgaatgat
      901 taaataaata ttattttaac agatattaat tataaagttt tgaatagtaa tttgttgaga
      961 aataaatata tccttataaa aatagatact tatataccaa gaataaaaaa tagtcaagat
     1021 agtacacaag gatatactta taaagaactc aaataagtga aataaattat taattataag
     1081 gtttataatt ttaaaaaatt agataaattc aggtgtaaaa tactataaaa cacaattcgg
     1141 aaaagtgtat gttgaacttc atttaaaatg gaatttcaaa gttgctatag taagtagttt
     1201 aagcattctt cttatattaa tctttgagaa taatgtattt aaaaaagtta gtaaaaaatt
     1261 taactattgt atattaatga tagttgctat tgatatatta tctaaaaaaa ttaagtatga
     1321 aaagattact gctaggacaa tcttttcata cttaattttt aatatttata ttatccaaaa
     1381 ttttcatctt tttaacttag aatttgagaa tcactattag gaaataggct ttgatttatg
     1441 tagggttaaa aatttcacag aatatctaac tttatactaa atccatcaaa aaatatacgc
     1501 catataaaag cgcataaaaa aacacataaa acaatattta tgcgcacttt aacctcgaat
     1561 ggtacaaaat tctttacttt tacagaccca tattatataa ttaagtaaat aatgtatatt
     1621 taggtgacat attggataag ctaatttatt tattttatta atataatgat actataaaag
     1681 ttatataaag tggtttacaa aaaagttaca gatagagctt ttattaatgt attgtgtatt
     1741 agtattttat ccttaggtac acttcaccta caaaatagaa tcataagata attatgggaa
     1801 aattttagga ggtttatcgt gaagaaaaag atattaatac cagtatttgc atcagttatg
     1861 gcattatcag taagttcaat tgtaaatgca gatgaagtaa atgattcatc tcaaaataag
     1921 gatgataaaa caaatactga attaaattta ggtgaatata aagaagtaaa atataaagtt
     1981 gcaaaaataa aagatggagt agctataaag ataagagaag aaggtcaagt tcaaaacata
     2041 gcttattctg gagatgaatt tacggtttta ggaactcaag gagaatgggt taaggttaaa
     2101 gttgaagatg gcgaaggttg gttagctact agatatgtag atatatctga aggtgtagga
     2161 tatactaacg ctgataaagt aaacttaaga aaagataaga gtgaaagttc agaagtaatt
     2221 gaagagctgg aaaaaggaag ttctttattg gttttggaag ataatggaga ttggttaaaa
     2281 gttaaagatg gggaaacaga aggatatgta aaatcatctt atatatctga taaagctcct
     2341 gtaatagaag agccacaagt aaacccaagt attgaccaaa atgtagattc tacaaataat
     2401 aatcaacaaa acaatgcaaa tactaatcaa caaaataata attcaaatgt gccaacagct
     2461 aactctaatg cagttcaagc tgtacttaac ttggcatatt ctaagcaagg ttgtccatat
     2521 gtttggggag cagaaggacc aaacactttt gactgttctg gatttacaca atatgtgtat
     2581 agaaatgcag taggaaagaa tataccaaga acatctaaag cacaatctaa atatggacaa
     2641 actgtaagta aagcaaattt acaaccagga gacttagtat tcttcacaac taatggttct
     2701 ggttctgtta gtcatgttgg aatatatgta ggtgggggta atatgataca ttcaccatca
     2761 acaggaaaaa cagttagtgt aactagtata aattcatcat actatacagc aagatttgtt
     2821 acagcaaaaa gaatattata atcggtttgt aatatgacaa attgataaat acataaatat
     2881 atataaacgc tctatattat atttaattta atcatggaaa caaaaaatga ttatatttta
     2941 aatgtactat agagcgtttt tgtatcactt taaatctaat caagattcaa gttaatatga
     3001 ctgttaaata ggagcctttt catatgatga ttatttgcgc aatcccttga caggataggg
     3061 caaaggtggt aagctactaa cagtacaaaa atacttttaa ttttagttca gagaggctaa
     3121 aaaaggagga cgaaagatgt taaaatcaag aaatttaatc cagccagaag acttttcaat
     3181 agaagaaata gatgaaatat tagaattagc tcaaaaaata attgacaatc cgtcaaaata
     3241 ttctcgtata tgtgaaggaa aactattagc tacattattt tatgaaccat caacaagaac
     3301 aagattaagc tttgaatctg ctatgaacag attaggagga cgtgtagtag gcttttcaga
     3361 gccaaattca tcctctgcat caaaaggtga aactctagga gatacaatga ggatagtatc
     3421 tggttatgtt gatataatag ctatgagaca tcctcaatca ggtgcagctt cagaagcagc
     3481 tagatatacg gaagtgccat ttataaatgc aggagacggc aaaaaccagc atccaactca
     3541 aacacttaca gacttactta ctataaaatc attaaaagga actttagaaa gccatacaat
     3601 aggattatgt ggtgatttaa aatatggaag aactgtgcat tctttggtta aggctatggc
     3661 aagatataaa aatacaaagt ttgtatttat agcgcctgaa gagttaaaaa tgccagatta
     3721 tataaaagaa gctataaagg gacatgcata ttatgaaact aataatttgg atgatgttat
     3781 aggaagcttg gatgttttat atatgactag agttcaacaa gaaagatttg aagataaatc
     3841 agagtatgaa agacttaaaa actattatat tttaaataaa gctaaattag aaaaggcatc
     3901 taaagatatg ttagtaatgc atccattacc aagagtaaat gaaatagaca tagatgtaga
     3961 tagtgatgat agagctgtat attttaaaca agctaaatat ggaatgtatg ttagaatggc
     4021 tttaataata aaacttttag gtataaatga agactaatag ttatcatata atagtaaaac
     4081 aaaggagatt ttgggatgta taaaattctg gaaaatatat atataggcga agatatgtat
     4141 agaatgaaag taaaaggtaa ttttgaaggt aaaatgggac aattctatat gttaagagca
     4201 tgggatactt atccagtact ttcaagacct ataagtatac atgatataga tgaagaaggg
     4261 ataacttttc tttataaagt agtaggagaa ggaactcaaa ttttgagtaa cctaaaggta
     4321 aatgataata taaaattaga aggcccttat ggtaatggat atgctaaggt tgatggaaag
     4381 gtagctctag taggaggagg aatcggtgta gctccactat atttggttgc taaaaatata
     4441 aaaaattgtg atgcatatct tgggtttaga gaagatgtta tattagaaga tgaatataaa
     4501 caagtatgta ataaagtcta tacaacagta ggaaatactt ttgtaacaga tataattgat
     4561 gttgagaaat atgattatat attaacatgt ggtccaactc caatgatgga aaaattagtt
     4621 aagatggtag aaggtactaa aacgagaatt atggtatctc ttgaaaacca tatggcttgt
     4681 ggagtaggtg cctgtttagt atgtacgtgt aaaactaatg gaggaaataa aaagacttgt
     4741 aaagatggtc cagtgttctg gggagaggac gtgattttta atgggtaatt taagtgtgaa
     4801 atttggaagt gtagaattta aaaatccagt aataatggcg tctggaactt ttggatttgg
     4861 aaaagaatat aatgagattt atgatataca aaaacttggt ggtataagta gcaagggtct
     4921 tactttaaat aaaaaacctg gtaacaatgg tatgagagtt catgagacat catccggtat
     4981 gatgaatagt gttgggcttg aaaaccctgg agttcaagga tttatagact atgaattacc
     5041 tttttttagt aaactagact tagtcagaat agctaatgtt ggaggtggga cattagaaga
     5101 ctacttatta ggggttcaaa tgttaaatga caagcctata gacattattg aattaaatat
     5161 atcttgccca aatgtcaaag ctggtggaat ggcttttggt ataaaaaatg aagtggcaag
     5221 agaagttgtt cgtgaggtaa gaaacataac gaaactgcct ttagtaataa aattatcacc
     5281 caatgcagaa gatattgtag gaatggcaaa agtttgtgaa gaagaaggtg ctgatggagt
     5341 ttcattagtt aatactttta aagcaatggc aattgatata aaaaatagaa gaccagtatt
     5401 tgaaaatgta tatgctgggt tatctggacc agctataaaa cctatagctt tgagaatggt
     5461 acatgaagtg tgtaaaaatg taaatatacc tgtaatggga atgggcggaa taactaaagc
     5521 cacagatgct atagagttta taatggcagg agctacttgt atccaagttg gaactgcaaa
     5581 ttttatgaat cctagaatag gtattgaaat aatagatgga attaatgaat ttatggatag
     5641 agaaggaata aaaagtttag acgaaataag aggaattata taaggtagaa taaatagtat
     5701 tttaaagata aaaaatatag attaaataaa caataatttt ataacattaa catggaggaa
     5761 aaataatatg agtaaagtag atgtagtgga tatattaaag aaaagtgatg cattgttgga
     5821 gggacatttt ttattatctt caggaaaaca cagtaataga tacgtacaat gtgcaaaagt
     5881 attaagattt ccagaatatg ctgctgaggt attaagtaca gttgttgaac aaataaaaga
     5941 cttagatata gacttagtag taggaccagc tatgggtgga gtaatagttt cttatgagtt
     6001 aggaagacaa ttaggaaaag aagctgtatt tactgagaga aaagacaata caatggagtt
     6061 aagaagagga tttgaagtta aaaaaggagc aaagataata attgctgaag acgttgtaac
     6121 tactggtaaa tcaactatgg agacaaaaag agtattagaa gccttaggtg gagaagttgt
     6181 aggtgttgca tgtatagcag atagaactaa tcatgatata ggtatgccta tatatagtgc
     6241 tataaaactt gatattcaag tttatgaatc tgatgagtgt cctttatgta aggaaggaaa
     6301 attaccagtt gttaagcctg gaagtagaga gttcaaggaa ttagggatgt aataaaaact
     6361 taattattta tagtgttact taaaaaatgt aaatttttta gagtaaaaat ttgtaatata
     6421 acatataact taaaaataaa aagcagttag ccttataaat gaaggttagc tgtttttttg
     6481 ttaaaaatat atttacaaaa taagagatag tagcagtaca atataagtat aaggtaaaag
     6541 tattatatat tagggagggg taagtatgaa aaatcaatta aatgaaaatc ataaaaaggt
     6601 tgtatttttt ggagtaggtg ctgtaggagc tacttttgca gaacaatttt ttaactctaa
     6661 atatgatttt aaaattcttt gtgataatga aagaaagaaa agatatttag aagaaggatt
     6721 tataataaat ggaaaaaggt atgattttga ttatgtaact aaagatgagt ataaacaaga
     6781 ggctgatttt ataattatag gtctaaaata taataattta aaagaaaata taaaagaatt
     6841 agatggatta gttggaaaaa atacagttat aatgtctctg ctaaatggag ttgatagcga
     6901 agagataata ggagaaagat ttggaattga aaaaatggta tattcatatg ttaccaatat
     6961 agatgcaaag aaaatcaata ataatattat acacactact aatgggataa ttgtatttgg
     7021 taacaaagat aatagtgaag atagaaaaac taatataata accgaagtct ttgatgatgt
     7081 aaatatagaa tatactttat caaaagatat tcaacgagat atgtggtgga agtacatggt
     7141 taatattggt gtaaatcaaa cttcagctat acttggtgca ccttatggag tttttcagag
     7201 ttctgagcat ttaagagaat tagcaaaatc tgcaatgagg gaagttgttg ctatagcaca
     7261 agcaaaagac atatctctta cagaagatga tgtggaacat tcattacata gaatactaga
     7321 acattcaaaa gaaggaagaa catcaatgct tcaagatgtg gaagctcata gacttacaga
     7381 agtagatatg ttttctaaga atatctgcaa acttggaaaa aaatataaca tacctactcc
     7441 tataaatcag actttctttt atatgataaa agtaattgaa agtagatttt aaagggtctt
     7501 tgtaagaaat attgataatt attgatttta aattttatta atgttatata ctatttaagt
     7561 tactaaatat ttttaactct tcaatgaaaa tagttgctgc ttttgataaa gttatatctt
     7621 ttggaaatat atatgagaaa tgtctgttgt agctgcttcc aagctctatg acagacagct
     7681 cattgtttag aacaggtaag cttgtcacta gattagagac tattgtaatt cctaaattat
     7741 ttctaacaga ttctttgaca gcataattac ttccaaaaac catcatactt tttggaataa
     7801 tttctttaac acttaaaaac atatctaaat aatctctggt tccagaacca tcctccctga
     7861 ctatccattt ttggttttga agtttatcaa aactaaaatc ttttaataaa tgactcttgt
     7921 atggaagtgc taaaaccatc ttatcttcaa aaaaatattc ttgaataaat gaaggggaag
     7981 agcaagtgcc ttcaataaga ccaatgtcta aaatataatc cttaacatga gaagatacga
     8041 ttgatgtatt ttttataaaa acttctacat ctatgtcagg atacttttta gaaaataatg
     8101 ctagaaaatt tggtaaaata tactctccaa tagttaaact tgcaccaatc tttaagtgac
     8161 cagttatagc atctgaaact tggcttacat catgataagt aatatttaaa agatttaata
     8221 tttctttagc tcttttgtac aagatttgtc cactttctgt tataaaaatg gttttttgtt
     8281 taatagacct gtttataagc acaactttaa aataattttc tagatttttt atgtgtttac
     8341 ttacacttgg ttgtgataga tttaaatatt ccccagcttt agtaaagttt ttgtattcaa
     8401 caacagctac aaatgttttt aattcttcaa acaatttttt gtctccttta cctaatgaaa
     8461 ttttacattt tttatatact ttctacagat aattatacta tatataataa ccattaaaaa
     8521 cattaatcat tacgatatta tatatttatt ttacttatgg ttattacaat tatatactat
     8581 tgctatgaag aaaactatat taattataag gtaggtatat tatgttaaag aaaaaaagca
     8641 gtttgacatt aaaaactata agggaaatat taccaggact gtttgtatca gtattagtag
     8701 gttatattag tatatttatt tctattttaa ttcctaaggt aggggcagca tcaatttcta
     8761 tatttttagg tatgtttgta ggaaacctat ttttaaatca aaaggtattt caaaaaggtt
     8821 ataaattttc agagacagat ttgttatcat attcaatagt actattagga gcaacactca
     8881 gtgtatctac cttaatagac ttaaaagttt caggaatttt atttataata ttgcaaatga
     8941 caataacaat aatagctgca ctttatatag gtaaaaaact aggatttgaa gaaaacttta
     9001 gatttctaat ggcaagtgga aatgcagtat gtggttcttc tgcaatagga gcaacagttc
     9061 cagtggttaa tgcgacagat aaagaaaagg gaattgcagt aactattgta aatgttactg
     9121 gtatatttct tatgttttta cttccaataa tatctcaatt tttatatagc catgaacttg
     9181 taaaaacttc agcaatgata ggtggaactt tgcaatcaat tggtcaggtt gtagcaagtg
     9241 gagcaattgt tggagaaaat gtaaaagatt tggctactat ttttaagata gtaagagtaa
     9301 tatttctagt tgttgttgta ttagtatttg gacatatgaa aaatcaatct aatagggaaa
     9361 ttcttgaaga agaaaaggat gagataaaaa agaaaaaagt gactatacca tggtatgtaa
     9421 ttggattctt tataacttgt gcactttttt caatgaacat tataccaaaa gaagtatctg
     9481 tgttgtgcaa agaaataagt aacaaactag aaataatagc tttagcagca attggattaa
     9541 aggttaatgt aaaagattta gtgaaacaag ggaaagaagt ttctttatat ggtttatttg
     9601 taggtatagt acaagttgta tcagctgtag ttttaataaa aatatttata tagcataaaa
     9661 atagtatttg taatattaat ttgatt
//
LOCUS       DAYAOX010000137         9424 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.137, whole genome shotgun sequence.
ACCESSION   DAYAOX010000137 DAYAOX010000000
VERSION     DAYAOX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.137"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(911..1549)
                     /locus_tag="ACGI57_002597"
     CDS             complement(911..1549)
                     /locus_tag="ACGI57_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFG9717667.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFTLWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(1552..2409)
                     /locus_tag="ACGI57_002598"
     CDS             complement(1552..2409)
                     /locus_tag="ACGI57_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717668.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(2411..2785)
                     /locus_tag="ACGI57_002599"
     CDS             complement(2411..2785)
                     /locus_tag="ACGI57_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9717669.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            complement(3947..5137)
                     /locus_tag="ACGI57_002600"
     CDS             complement(3947..5137)
                     /locus_tag="ACGI57_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFG9717670.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(5153..7108)
                     /locus_tag="ACGI57_002601"
     CDS             complement(5153..7108)
                     /locus_tag="ACGI57_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFG9717671.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(7125..9170)
                     /locus_tag="ACGI57_002602"
     CDS             complement(7125..9170)
                     /locus_tag="ACGI57_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFG9717672.1"
                     /translation="MKKITKERIENTMKKLFTSETFLEYQFISDCQISPDGGYTAFIV
                     KKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRNEKVKEAV
                     KNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRPIVEGLPE
                     EEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAITAPMFNV
                     VGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGFELYQNQV
                     ILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGAGQTTKVD
                     GEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCLGNGLPEV
                     YTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYEVGKKYPA
                     VLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQFGEIDYI
                     DFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRSIANWSGM
                     EGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYVDKCVTPTLFLHGEKDYRCYMQEA
                     FQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIKKDNIGQ"
BASE COUNT         3216 a         1671 c         1112 g         3425 t
ORIGIN      
        1 ttaataccta gtatatcact taagttgaag gattactcga cttgattttt ttgttatttt
       61 atggtatagt aattaatata gaagtaagta attgcaagct ttgcaattac ttttattttt
      121 ttctaataac tattttattt ttgataattc gtataaaatt aaaagtaaag taatatggaa
      181 aatatgaata tagaaaaact ttaataagaa aataaattat gaataaataa aagaaacgca
      241 atactgtata tagatgatta tttagaaaaa taaaatctct tttaataaaa ataagcttca
      301 aaataaactt ttagttcata aagcttagga ggaactaaat tgagtgcaag tgtatctact
      361 gttcaaaata aatagttcat aaagcactct tttttatatt aaatcaatta tattttcgtc
      421 atttcagcaa tgaaaaagaa acttatctct attttgagac agcttctttt tattaacttc
      481 ctacttttta ttagttttct aaaattgaaa agctttattt ataggattcc ttagatttat
      541 attttgacga tataattttt taaaaacatc ttaaaagatg gtatacaata cacacaatca
      601 tactcataat tgaaaaataa tcaatggaaa ataaaattac tggatattat ctattaaaaa
      661 atacaaattg attcacaaga aacatataat ctataatatt atgatgttac tctctcgcac
      721 caagtaactt tattttcaag taacctttta aattcatacc atctaacttc actcaataaa
      781 aatggcaata taaatatgta ctctattttt tacttttaaa tatctgacaa gctttatata
      841 ctattggaag taaaatacaa agtaaaacag cccaatctgg taaatgtggc atataaaagt
      901 cccccttacc ttatacaagc tccttattac taaaaatttt aatagaaata aacattgaag
      961 ctaacagtat aattaaactt aaaaggaaca atacaatatt cactatcatg gatggatact
     1021 gatatataaa tggaatttca aaattgctgt gagctatagt taatggtaat acaaatgatg
     1081 aagccatgat tatcaccacg aaaaagaatt ttgttgtttc atatcctaat ttatattgaa
     1141 taggcatata aactccataa ataatagaac ttattaataa aacaatcaaa atatatgtta
     1201 agtcaaaagt tcctatttgt ggaataagta aattttcaat ccagtaaact aatgtacaat
     1261 agataaatac cattgcaaat aaaacatact tagattgaac taagccacgt ctaggataag
     1321 gagttgaaca aagtaataca gatgcttttg aatattgact ctcctttaat aatagatact
     1381 ggcacagcat aaattctgaa aatataatgg atagtacaaa tcccaatatt tgtgcaaatg
     1441 gaggtaaacg ccacaatgta aataatggga ttcctattgc aataaacaat atcaacaata
     1501 catatttttt tacaatcaga atatcttttt taactaaatg aatcaacata attactctct
     1561 cctttcaata tatgcaagca taatatcttc aattgtagga cgctctatta aaatatctgg
     1621 catatttttt tgcacttcat taatttttga ggtaatccct ataaaaccat aacctgtaat
     1681 ttctaaattt aaaaataatt ttttgatttc atcatttaga aattgaatat ccccttttac
     1741 ttttctatgt tcgtccaata gagtatcttt ttcacgctct aatataatac gtccattgtc
     1801 aatcataaca atcatatctg ctattttgtc taaatcagat gttatatgag tggaaaagaa
     1861 aacaccacga ccacattttt tcatatatcc tgttagtatg tcaagtatct gactacggat
     1921 tagtgggtct aaaccacttg taggttcatc cataattaaa aactcagctt tatgcgataa
     1981 tgctaatgct aatgcaaatt tcattttcat tccttttgaa agtgtatcaa tcttttgttt
     2041 tgggttaaga ttaaaacgct tcatattttg ttgataatct tcttcacacc aattatcata
     2101 tgaaggtgca ataatacttt tcatttctgc cattgacaac tcctcataga aatatgaatc
     2161 atcaaataca actccaattc gattttttaa ttccttctca tttccatcga aatctttacc
     2221 aaagattttt atttttccac tatcacgctt tacgacatcc aatatcgaac ggattgttgt
     2281 tgtttttcct gctccattaa cacctataaa tccagttata caatcttctg gtaatgcaaa
     2341 gcttatatta gataatgaaa aatcggtata agtcttattt aagttataaa cttctaaaat
     2401 attttccata ttaatcctcc tgatatagaa tatccatcat ttcttgtaac tctatctgtg
     2461 taattcctag tgcttttgca tccttaatca tatctgccat tttttgttca actatacgtc
     2521 tctttgagtc tcttagtatt tctaaatttc cagttgaaat aaatgttcct attccaactt
     2581 ggcgtgtaag aaagccttca ttttcaagtt cttcatacac tcgtctaata gtcaaaatac
     2641 taacttttaa atcattagca aaatttctta tggatggcat tgcatcccct tctttcagtt
     2701 cttcagtaaa tattgcttct ttaatttgct ctttaatttg ttggtataat ggcttatctg
     2761 atgtgttaga aatgagcact ttcatttttt atcaccccct ttttatcatt ttcagtgtga
     2821 tgttgttata tacacactat acacttagag cacttttttg tcaatataaa agatgtaaaa
     2881 tattactatg acaaaataaa tatgaatgaa atattagaga ttgttgatag ctataaacac
     2941 tttttaaaac cacataagat agaatgtatt aaatatttat taaaagtaag aattgaatat
     3001 ctaaaaaaaa gaggaatcct gtatgaatga tttgtttgat gataaattaa gttataaact
     3061 atttataaaa aacataaagg aataaatcca tttctaatta caaacatact aaaaaattta
     3121 gatagtaata aatagatagt aataaataga tagtaataaa tgggatgaat ttataagaat
     3181 ttggatagag gaaagagtta ttcaagagtg tagactagag gtaaactcta tattaaaaaa
     3241 atttaaattt ggatattatg atgcatggaa tatttgcaga agtaaccttg ctataagtat
     3301 agaagatttt tttgattaca aaagataata ctctaaaatg tgaggatttc aacattcaat
     3361 actgtacttt aaataatatc actgcaaaat accatccacc ttttaccata attgaatacc
     3421 ctaattattt taaaaaagaa aacattagaa acagaaaacc ttggatatat aaaataaaaa
     3481 atactaactt ctaaaacacg gatactcttt gataaataaa acaaaaaata attttaataa
     3541 ttaaaattgg ctatctcaaa ctagagataa gtctctttct cacttttaaa acgttgaaaa
     3601 tatattcgat ttcgtacaaa aaagagtgcc ttagaactaa aaagttcact ttgtggcact
     3661 cccttttttc tcctcttttt aattttttat aaaaattctt caaaaatatt attaaatacc
     3721 ctatattata cataaatcaa attaaagttg cattttagca aataaaaaca aattataact
     3781 attagcatag caaataaaaa atctgctctc ttataaaatc aaattataat tataaaaaga
     3841 aaatattctt ataacaaatt agctaatata aaattatttt ataaattaat actacttttt
     3901 agcttctcct ataaattaat accacttttt taactttgaa acctatctac aacattggta
     3961 ccataagacc agctaccaca atagaaatca tacaagccat accaccaact accagcttag
     4021 gcataatctt agacatcaaa tatgctcttt cttcttccgt ttctccaaca gcattacaca
     4081 cttcatttga aattaaatat gtagttggga atcccaacat ctgacaaaac cctactccaa
     4141 aagcaagagg tttagaaccg ataattttcc aacctggtaa caccttcatc ataaggaata
     4201 taccaatgat agaagctgca aacataccca caattggtac aaaaagacta attaaatctg
     4261 tcaaagaaac ttttgctaaa gaaggaataa cagctgcaaa cacaaccatg ttgataaatc
     4321 ccttgatttt tcctttttcc aaaatgtctt ttggcacaat accaatacgt gttacaataa
     4381 caccaacaac aagacataca atagaataat ttacatgaat aatctctgaa agttcatacc
     4441 ctagataaga gaataatgct ataaaaaata tacaaacatt tgtagaataa tatttatcgt
     4501 atttttcaca gaaagcttgt tttacttttg tgcctgtatt ctctgcttct ttcttatcag
     4561 tatcaacatt tgctaaatca atgccttttg attttgcttc atgatattct actaaaagac
     4621 ggtttgcctc ttgtaacgct gctcttgaag ctattggtgt accaataaac ttctgaattg
     4681 caaatacaac tgttgctgta gctgctgcaa gtgtcagtcc tttttctagt gctgcttgtg
     4741 tcataatagt agtagcaggt aaagctccat ttataactgg tatgcttgtc aatactgttg
     4801 attttccaaa caaaggaata cataatgaga cacagactat aactgctaca attccaagcc
     4861 atgctgtcaa tactgttctc cattcgtcta taaattgttt aacattcaac attgttccca
     4921 tactaaacat aatcattgga acactccaac tagctgctgc ggtcatacct gctttttcaa
     4981 taatatcagc tggtattgct tttgtgacaa atagaatcaa aaataaaagt agtgtaacaa
     5041 atacagctga tacttttgct tttgttttgc ttgcaattaa atccccaata gcaaataaaa
     5101 ctaatacgac actaaaccat aataacatat cactcataga ataaccccct tattataaat
     5161 atctttcaaa ccaatctaca atagcatttg ctcttgcaat tctattagat ggtcttccat
     5221 ttctagacaa tccatgtgta tctccgtgga acaaatacat ttctgtatct acaccaagct
     5281 gttttaatgc tgtaaacatt tggaaagtat cccccataaa acatctataa tccctatcac
     5341 tatgtaagaa caatgttggt gtcttaacat ttttcacata tttaataggt gaatgattcc
     5401 aaagaacctc ctcatgattc caaggcgtct tatctccaca agtctgcttc ataattctat
     5461 ccccaaagtc aataattaaa gaagctgtaa tcaagttact aatacctctt tgagaaactg
     5521 ctgctttgaa acggtctgta tgaccaatta tccagtttgt catataacca ccgtaacttc
     5581 cccctgcaac ccctaagcgg tctgcatcaa tcccaggata tttttcaata caagtatcta
     5641 caaattccat aagatgctca tagtcaacat acccccatct ttctaccatt tctaagaatt
     5701 tttcaccata accgtcacca ccatgagggt tacaataaat cacatacatt ccctttgatg
     5761 ttacacactg cttcaagtgt gtaaacatat agccatatgc ccatttaggc ccaccatgaa
     5821 tgaacaatac agcaggatat ttttcgcctt ctttagcatc tataggtggt ataacaaaac
     5881 catcaacttc ttctttattt tttgctacaa acgtcaattt ttcaggtaat gaaaccttat
     5941 gagtatctaa atatggttta gaaaaagcag tcattttttt cattgtacct gtagtcatat
     6001 caatgctata tatttctgct aactcataat ctttcaacat tactgtgtac atggtgttgt
     6061 cataaatatc aaaagatgta aaagcatcta cttttgtgat gatacgtata ttacctgtct
     6121 ttgtatcaaa ctcattaaat tctgtcatag ttctgcaaat tcttgcacca ataaatttat
     6181 ctcctataac tttattacta cgtccaccac catatactgc atctacatcc agtaatgtag
     6241 catacatacc atctggacta gcaatcatat ttatttcact agtactcaaa tcaagtataa
     6301 aaatatcatg attctgtgta atagaacggt ctaatgttga agcttgcaac attactttat
     6361 ctttgccaat atattttgcc aaacttattt ggtactttcc ttgtggtaca agttctttgg
     6421 tttcaccact gtctatatga tactcccaaa gtccagaaaa aggagttgca caatcttcat
     6481 aaatctgacc agtatataaa attctgtttt tatccgaaga aacatctata cctgctactt
     6541 cacaaaattt tggtgtaatg gcttttgtta tattttcacc ttcttgatat aattctaatg
     6601 ttacacgtct tctttgagaa aaattttctc catctcttat aaaaggttta tctgtaaatg
     6661 tccaataatc tacaccctct actgccctat ctggttctga ttttttagaa tccgttggat
     6721 tttttgttgt cagtaaccac aatttatttc caagtgatac aggtacgcta caagcttttg
     6781 gaatagaaaa tgcttttttt gcttctcctc cttctataga aatagtgtaa aaatctgttg
     6841 tacctgcttt tggcttattt cttttagatg taaaaagtag ttctgtatta gaaatccaac
     6901 taaatgatgt tacatctttt gtttgagtca tatgtatttg tttttctgtt ttgatgtcca
     6961 tcacatataa gtcatgttca tagcagtctt tttcctcgtt gatatttgat ataatataag
     7021 caatatgctt tccatcaggt gaacaagaaa tatcatgagg gaatatatat tcaaaaatat
     7081 ctgaactttc aatctttatt ttttccattt ttatattctc ctttctactg tcctatgtta
     7141 tcttttttta tatataccga aaaccaatct agcatttcaa ctaatctttg tagcttttgt
     7201 tttggtctac cagaacgaga cagttcatgg ttttctcctt caaatagaca aagttttgta
     7261 ggacacccat gaattttcaa tgctgaaaac atctggaatg cttcttgcat atagcatcta
     7321 tagtcttttt caccatgtaa aaataaagtc ggtgtaacac atttatcaac ataagcaaga
     7381 ggtgactgtt tccattgaag ctcgtggttt tccatatgag aagcacctgt ttgtcctttg
     7441 cagaaataat aaccaatgtc tgttgtaccc tccatgccac tccagtttgc aatagaacgc
     7501 tgagcacaag caacagcaaa acggtttgta tgaccaatta tccaattcgt cataaatcca
     7561 ccataacttc caccttcaac agccaatttt gttttatcaa tgtctgtata tttttcaagt
     7621 acagtatctg taaattccat aaaatctata taatcaattt ctccaaactg tttcctaata
     7681 tctgcaaaag cattgccacg accttcagac ccccttgggt tacagaagaa tacaaagtaa
     7741 cctgcacttg taaatacctg tatctcatgc ataaaatatg gtccatatgt caatcttggt
     7801 cctccatgaa tcgccaatac agctggatat tttttcccca cctcataatc tactggttta
     7861 tatacgtatc cttgtatttc ccatttagaa cttccttttg actctatgta ttctggcaca
     7921 gaaattttat attcctctaa aatatggtca tttaaatgtg ttttcttaca caatgttcca
     7981 ttctctactg tataaacttc tggtaatcca tttcctaaac aacccacaca tacaatattg
     8041 ccatctttta catctataaa atcaactgaa cctgtctgtg ttactttttc ctgttttcct
     8101 gttggaatat gtattctttc aataatgcag tccatatcta ctgtagaagt gtaataaata
     8161 tattctccat ctacctttgt tgtctgacca gcacccattt taacatcaga tgttactgta
     8221 tttccaatac aatgatgttg atgccctgaa agaagtttca tttctttcgt atcaatatca
     8281 atagtataaa aatctccatt ttctccattt ccataagata aagcatcagt taggtttagt
     8341 atcacttgat tttgatataa ttcaaaacct tttataatat acttatcttt ttctaatata
     8401 cagacatttg tgtttgtgtt tgtatcaaat acatagaccc catttttcaa accttttaca
     8461 tctttaaatt cttgccctat atataagatt tttccatcac ttattttcat acctacaaca
     8521 ttaaacatag gtgctgtaat tgccttcaac tcatttatat tactatcgta tacatatagg
     8581 cgttttcttt ttctgctaat atatccctcc ccattgacag cataaggaat ttctttgaaa
     8641 tgttcataat gtcttttgtt ataagcattt atttttttct gttgttcttc ttctggcaat
     8701 ccctctacaa taggtcggtc attgtcataa gaatcaataa ctgcatacaa accttttcct
     8761 ataggattca atttaccacc tttgataggg agtctaaaaa gttcttctgc ttctccacca
     8821 tttaaagata aagcatagta actcatgcat tgcttaccgt ttttgacagc ttcttttact
     8881 ttttcatttc gcaaagcagg gaacaagata gtattttcat cttcccaggc atatgctcca
     8941 acagagttta tagacgttat ttgttttaat tcgccagttt tattgtttaa tatataaact
     9001 tggcttgtgt aaccattctc tttgatatca gcttttttta caataaatgc tgtatatcct
     9061 ccatctggtg atatttggca atcacttata aattgatatt ccaaaaatgt ttcactcgta
     9121 aatagctttt tcatagtatt ttctatcctt tctttagtaa tttttttcaa actccctacc
     9181 acctctttca ttttttattt taaatttaaa ataaaaaatt ttaaatttaa aataaaaaat
     9241 tttataaagg ttattttttt aggaagcctt ctggcttcct tgtaatattt gtaatttaaa
     9301 gtatatttaa tccatgctta attcatgctt aattcatact taatctatac ttaatctata
     9361 cttagtccat acttaatcta tatttaattc atacttagac ttatttacac tatatttaga
     9421 ttat
//
LOCUS       DAYAOX010000138         9423 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.138, whole genome shotgun sequence.
ACCESSION   DAYAOX010000138 DAYAOX010000000
VERSION     DAYAOX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9423
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.138"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            33..569
                     /locus_tag="ACGI57_002603"
     CDS             33..569
                     /locus_tag="ACGI57_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9717673.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            685..1392
                     /locus_tag="ACGI57_002604"
     CDS             685..1392
                     /locus_tag="ACGI57_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HFG9717674.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            1394..2110
                     /locus_tag="ACGI57_002605"
     CDS             1394..2110
                     /locus_tag="ACGI57_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HFG9717675.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            2112..2870
                     /locus_tag="ACGI57_002606"
     CDS             2112..2870
                     /locus_tag="ACGI57_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HFG9717676.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            3005..4393
                     /locus_tag="ACGI57_002607"
     CDS             3005..4393
                     /locus_tag="ACGI57_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HFG9717677.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(4469..5290)
                     /locus_tag="ACGI57_002608"
     CDS             complement(4469..5290)
                     /locus_tag="ACGI57_002608"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HFG9717678.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            5541..6695
                     /locus_tag="ACGI57_002609"
     CDS             5541..6695
                     /locus_tag="ACGI57_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HFG9717679.1"
                     /translation="MKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNMEEKVM
                     ESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYENEVKK
                     MKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQKDKENA
                     FRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGVELQLT
                     DEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGRFKKNS
                     IWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFVGYEKK
                     GRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            6878..7078
                     /locus_tag="ACGI57_002610"
     CDS             6878..7078
                     /locus_tag="ACGI57_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFG9717680.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            7499..7927
                     /locus_tag="ACGI57_002611"
     CDS             7499..7927
                     /locus_tag="ACGI57_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFG9717681.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(8070..8825)
                     /locus_tag="ACGI57_002612"
     CDS             complement(8070..8825)
                     /locus_tag="ACGI57_002612"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9717682.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
BASE COUNT         3622 a         1052 c         1499 g         3250 t
ORIGIN      
        1 agtatcgaaa gagaattagg agagggatac ttatgaataa aatagcagta agttttttaa
       61 taattgctac tactttattg agtacggcat gtatggatta tagcatcagt gcagttgagc
      121 tagttgattc taaagaatca gcagttgtaa agaaggatga agatgcaaaa gaagaaacaa
      181 ctagtaaaat gattaattct aaaaaaacta ctaaaatacc tatagagata atttcaaagg
      241 atgagaaaat agtaaagtac ttacagattg atgaagaaag ttcacttaag gataaactgc
      301 gattaatctt agatacatta tcaaatgagt attttaatgg tttagacatg gaagtagaag
      361 ttaaggaaaa agataatctt gttaaaataa acttgataga accagataaa aaaagtagag
      421 tttcttggaa agatgattat ttgaatgaac aaaatataat atatactata aataatatta
      481 ttaaaaatgt catccaagaa gaagataata gcatttggat tgaagaagta gagatatact
      541 acaatggaaa attaatagaa ttaagataaa aaaagatgtg tttttaacac atcttttttt
      601 gttttttaat tcttataaaa tccttatatt tatgatttag aatgattctt gtaaataagt
      661 ttgacaaaag aaaggatgaa aattatgagt aattatattt tgcaggtaaa aggagtttct
      721 aaaaaattta aggagcagat ggcagttgat aacatttcac tagcaattaa atctaattct
      781 atatatggat tattaggacc aaatggagct gggaaatcta ctttattaaa aatgataact
      841 gggattttaa atccaacttc aggaaaaata atgtttgagg gacatagatg gactagaaag
      901 gatttatcaa atattggttc tcttattgag tctcctgcta tatatgagaa tttaacagca
      961 cgagaaaatt taaaagtcca tactacttta cttggtcttc ctgactctcg cattgaagaa
     1021 gttttagaga ctgtagattt aagagatacc tttaaaaagc gttcaggtca attctctcta
     1081 ggcatgaagc aacgccttgg tattgctatt gcattattaa atcacccaaa acttttaatc
     1141 ttagatgagc ctacaaatgg tcttgaccct attggtattg aagaacttag agaattgatt
     1201 cgttcttttc caaaacaagg aattacagtt attttatcaa gccatatctt gtctgaagtt
     1261 gaacaagttg tagatgaaat aggaataatt tctaatggag taatgggtta tcaaggagaa
     1321 gtatcaaaag aacaaaactt agaagaactt tttatgaaag ttgtagctga aaatagaaag
     1381 agagggcagt aatatggaaa gatttattag agtggaattt caaaagatga aaggaacttt
     1441 tggaatgaag ttgatatgga tagttccagt gttttgtata cttttgtgtg gactaggtgg
     1501 agggcaaaat gctgctttca actggtggta tacgacattt cttccaggag cattaacatt
     1561 aatatcttct ttaatagtat taaaagatga aaaaagtaaa tatcgtggta ttttaccatt
     1621 gccaattgaa atgaaagacg tttggactgg aaaaatgatt tactgtatta tttgttgttt
     1681 tatagcatca attatttctg caataggtgt agtttttgtt gggataatct ataaagaaac
     1741 tatttcaata ggtcaaaata tcattgcaag tatattaatt attttactat ttatgtggca
     1801 gattcctctt tgtatgtttt tagcttcaaa atttggtatg tttgttacag ttctaacaaa
     1861 tatgttcttt actattgttg gagttgctgt tttctcaact acagaaatgt ggtatatgtt
     1921 tccatattcg attccttcaa gattaatggc tgttgtattg catattctgc caaatggtct
     1981 tcctgtacca gagggaagtg aatttttaaa taatgctgtt attttgccag gagtattaat
     2041 atctattgtt ttatttgtat tatttgcttt agctacaagg gtttggttca ataatagaga
     2101 ggtgaagtaa tatgagacag ttaataagaa tattttgttc tgatttaata aaattaaaga
     2161 gaaattttat aatactaatg cacatttgca ttgctttaat ggggatggga ttgtttttat
     2221 ggtattataa ggtttcagga gctgataata tactaaagat agtagcatat ttacaagtta
     2281 tagcaatagc ttttccttta ttgtcaagtg ttgtgtgttc aatgtttgtt gaacaagaat
     2341 attatgcagg gaattataaa catatgttaa caacatctaa tccaaaatat cttactctta
     2401 taagtaaata tataatctta gtatgtcttg gttttgtagc aacattagtg tctgtattag
     2461 gttttaaatt tggtatatct aaggcttact ttacatcaag cttttatatg atttcaattc
     2521 taattttaat tgggtgtaat ttatttgaat acatattaca tttattctta agtttaaggt
     2581 tcggaaaagg aacatcaatt ggagtaggta tagttgaaat gttactttca gcattattat
     2641 taactggatt gggttctggt atttggcagt attttccttg tgcatggggt gttagattta
     2701 taactatttg gtcgtctttt tcaagttcta aaacggttga atatataaag gttgaatcaa
     2761 taaaagcata tcaatcaaca ggtttaattt gtggttttgt aacaatttta gcatttgtaa
     2821 ttttatgtat ttggttttct aaatgggaag gtaaaaaatc agaagaatag agtaaaaatt
     2881 gactgtttat ttatattatt atgttaaaat ataacttagc aataaaaaga tataaaataa
     2941 tcaaataaaa aactggttgg gtattaatta tgaattaatt aaactattgg aggacagggt
     3001 acaaatgctt ggaggtaaaa aatctaacaa caaaaaagtt gaaaagttaa gaactatttt
     3061 tataaaatat ttatctttat tctttattat gacaatttca attgtactcc ttttaatgtt
     3121 atcattttct gtattattat catcaggtgt aattttacca gctaattatg cagaaaaaca
     3181 atttaataaa tataaagaac agattatttc aagtgaaaaa gtaactgaag atataattcc
     3241 aagtatatat gagtatgggg tttatacttt ggatgggaat ttaatttcag ggacctttaa
     3301 taaaaaagaa tcaaaagaag tatggaattt aatgagagat attgaagaaa ggcatgcata
     3361 ttcagaaagt tatataaagt tttttaagaa agatgaagtt tttataataa aatacaaaat
     3421 agtatcagag tatagttctc caattttaag agcatattta ccaaaaccag agacattggg
     3481 tatgattatt tttagtataa tattttttat tgaaattgtt atattatcaa aagtatttgg
     3541 caagaaattt aatattgaaa tggagctttt aaaaaatact acagaaaaaa tagaacaaca
     3601 ggatttagat tttgttgtag aatctagtaa aatttatgaa attaataatg tccttttttc
     3661 tatggataaa atgaagttgg cgttaaaaga ttctttagaa aaacaatgga tgctagaaga
     3721 aaatagaaag gaacagatat ctgcactggc acatgatata aaaacaccac taacaattat
     3781 acatggaaat actgacttat taattgaaat aaatgaaaat cctgagttat tagaatacat
     3841 ggaatatatc tcaaagggtg tcactcaaat agaaaaatat attaatactt taatagatat
     3901 atcaaaaaca gagaatggct atatattaaa taaagaagca atcagtatga gtgagtttat
     3961 aaaagatgtt ttagtgcaaa tagaagcatt agctagtact aaaaacttag atgtggagtt
     4021 ttataaacaa gataatttgc ctaaaaatat aactatagat agagaactgt tattcagagc
     4081 tattatgaat ataatttcaa atgctataga ttattcacca agccagagta aactatacat
     4141 aagtgtatca gtacataata aatacttaaa atttgtagtt acagattgtg gaagtggttt
     4201 ttctaaagca gatttgtcaa aagctacaga acagttttat acaggagatt tgagtagaaa
     4261 ttctaaatct cattatggaa tgggactcta tattgtaaat aatattgtac aaaaacataa
     4321 tgggacttta tgtattgaaa attctgtgaa aactggtggt gctatggtaa caattgaaat
     4381 accaatagtt tagtaaataa tatccatttt gtaaatacct aaattttatg atttataggt
     4441 atttataaaa tggatagttt ttttgatttt atatattggt taaatctgat aaaaagtttt
     4501 caaacataac tccatcattt ctatcagtat caaattgaga tgtgtaattt atagttttat
     4561 atataaaatc tgttgcagta ttaaccgcta ctcccaaatc atgtttattt actaacatac
     4621 cacaaagtat agatgtgaat atatctccag ttccactgta agaacaatta ttatatttga
     4681 ctgatgtaaa gaatacatgg tcattatctc tattgtaagc caaatttaat atattatcat
     4741 cttctaatat accagtaata acgactttgc taggtcccaa atcagataca gattttgcaa
     4801 tatatataag ctcatctcta ttataatcgc ttttggtata atcatttcca gttaagaaac
     4861 aagcttctgt taaattagga gtagttaaat ctgaatgttt aactaattct ttaatttcct
     4921 gtctcatttc ttctgtgaat attggataca taactccatt atcacccatc acagggtcaa
     4981 ctacgataaa agcattggga taatcgttta taaaattagc cactatctca atttgatgct
     5041 ttgaacccaa aaaaccacta tatatactat caaaattgac ttttaaattt ttccaaacat
     5101 ttgaatattt taccatttca tcagtaaagt ctaagaaagt aaattcagga taaccagttt
     5161 gacttgaaag tatggccgtt ggaaatggac aacattgaac ctttaatgct gaaagtatag
     5221 gtatagcaac cgataatgag cattttccta ttccagataa gtcattaatt gctgcgactt
     5281 ttttttgcat aaaaaaccct ccattagtat gtattaatta taatgatacc aaaatattta
     5341 tatacatata tattactata aaaaaaagaa aaaagtagat tataaaataa aaaattgtaa
     5401 aaaaaacagt gtattgatac aattaatttt taatattgga cataataaaa tagagaatta
     5461 ttaaatatga aaattactat atgtgacaga atattctttg taatttgatt tgtaaggtgt
     5521 actatttggg aggtgtagct ttgaaaatac tgaaaaataa gaataagtac atcaaaggta
     5581 taatggcatt tgtatttttg tctttagcta ctacagcagg tgtattttta atcaatgatt
     5641 taagtatgaa aaatatggaa gaaaaagtca tggaatctgc aaatgttgaa ggtgatgatg
     5701 taaaagacca atataaacta ttgcttgaaa acttatttga ctacagaaat aaggcaatat
     5761 tagagaaaaa tgaaaatatt ttaaaaggtc tttatgaaac ggataaaaaa tttggtctgt
     5821 gggcttatga aaatgaagta aaaaagatga aatatttaga aaactggtct agtaaacaag
     5881 gtgtaaaatt taaagacata aagacaaggg ttaagattaa aaaaataaag gaaaaagaaa
     5941 aagacctcta tggtataatc tgttcagtat ccactgaata taagtattca tatgaaaatc
     6001 aaaaggacaa agaaaatgcc tttagaatag gtacatatca ttatctaaat gtaaaaatta
     6061 tagataatca atatgtcata actaaagagt ggtatacaga cccttttgca gactctttaa
     6121 atttagaaaa tataaaatct gatgatataa gaaattatat tggtcaacaa gatggtgtag
     6181 agttacagct tacagatgaa caaaaaaaag ctatagatta tgcacataaa tactgtggag
     6241 ccgcagctga tgaagaacat ggtatgaaat ttaatccaaa ctatagggat tataatccag
     6301 aaggtggaga ttgtgcaaac tttgcatcac agataatgtt tgaaagtgga aggttcaaaa
     6361 agaactcaat ttggaattat agcgagagag caggtacaaa agcatgggta aatgcacaag
     6421 cttttaaaaa ttatgcactg tatagtggaa gaggttcatt aattgctaaa ggttcctatg
     6481 aagaagtata caaagaagct tataatttac gtccagggga ttttgttggt tatgagaaga
     6541 aaggcagaat aacacatgtt tcgacagtga caggattaga ctcaagagga tatcctttgg
     6601 taacttgcca taatactgat agaatgttgg tgccatggga tttaggctgg agtgataagt
     6661 ccataagatt ccatataata aaaataaatt attaaaaaat tataaagaaa aataaagtgt
     6721 aaaagataat aatcatttaa aattataaag taaaaaaatt gattattaat gtataaaaat
     6781 aataataatt gacaaacata gctatatatg atagagtagt aataaggtta cagaaaagag
     6841 tagctaaata tttgtgtttt tttaggagga tttattaatg aaaactggtg tagttaaatg
     6901 gtttaacaac gaaaaaggat ttggattcat atctgtagaa ggtgaaggag atgttttcgt
     6961 acatttctca gctataactg gagatggata caaaacatta gaagaaggac aaagcgttga
     7021 gttcgaagta gtagatggag ctaaaggtcc tcaagctgct aacgtagtta gattataatt
     7081 aaaaattcat atatattagt gttctagtaa tatgagagat aagccgctaa tcatttagcg
     7141 gttttttcag tttttgtaaa taaaattaat agcaaaataa attaaaatta ttaaaataaa
     7201 atcattaata atgagataaa tatgttaaaa ttaatagtgt agagtaaaat tattatggct
     7261 aagtttaagt caattaattt ttagtaaatt ttctaaaaag agaataagtg atacttatag
     7321 accatataaa aatattaata aagcataata agattaaaat gagcaaagga aaaacgcttg
     7381 ttacagcgta tagtattaag aatacttaga aaataagaaa ggagaaagtt actgcaaaaa
     7441 agtaacatta taaatattga gtaatattta taagttttca gtaacggtgg gtagtgtaat
     7501 gaacatatta ttctttctaa caccaaaaag tgaagtagca tatatatatg aagactacac
     7561 tataaggcaa gccctagaaa aaatggaata tcacaaatac tctgcaattc caataataag
     7621 taaggatggt aaatatgttg gaactataac agaaggtgat tttttatgga cattaaaaaa
     7681 tgatttaaat ctggacttaa agggattgga agatgttcca gtgacagata taaatagaaa
     7741 aatggataat tctcctgttt ccatcaacgc agatattgaa gatttagtta taaaatcttt
     7801 aaatcaaaat ttcattcctg ttatagatga ccaagacaca tttattggga taataaaaag
     7861 aagagatgta ataggttact gttatgagat aatacgtgga tataaaaatt tagctaatga
     7921 taattaaaaa tcttatttaa acttttaagc tgagttcttt acaataaaaa aatgataata
     7981 tgaaactgaa tatttaaact ttaatataaa aagctgtctt ttctaataaa tattgtgaaa
     8041 agacagcttt tttatatatg accttatgat taatcgttgt attttacaag gtttccgtac
     8101 ttagcattaa gagcatttat cttatttcca taaacttcat tttgtttttg atataataaa
     8161 cttttttcaa cttcagcctt aacttcatca aatgtagact ctgtactttc ttgaaggtct
     8221 tctaatttta ttatatggta tccaaattga gttttaactg gttcacttac ttcaccttta
     8281 gccatagaga acacagcttc ttcaaattca ggaaccattt gtcctctacc gaatgttcct
     8341 aaatcaccac ccatatcttt agatgggcaa gaagaatgtt taagagctgc atcttcaaaa
     8401 gatatttctt ctgattttat ttgagcaagt atttctttag ctttttcatc actatctact
     8461 aatatatgtt tagcagttgc actttctggt ttagagaaag agctcttatt agcttcaaag
     8521 aattttactt tttcatcttc tgttacatta acactagata aaactttatt tatagcaaat
     8581 tgctttaata cattttcttc tatctttttc atttctaatt taaatatttc ttcttcatct
     8641 aatttttctt ctttagcatc taagaagaat aattcttgat tcactaaatc atttaataag
     8701 tgtttttttc cttcttcagt tttaaattgc atagcttgat aagggtctaa tgattttaat
     8761 gcattttcaa tatcaatatt agttatctct ttttctccaa ctgttgctaa aacttttttt
     8821 tccataatag ttaactcctt catagtgtat ttcattatta atataagact accatcttaa
     8881 ataatatcag aaaataatgt agatgtctaa tatttaaagc catattaagt caaatagaat
     8941 taaatttagt agaacaaagt tattacaatt aataaaagtg agctcataat acccactaca
     9001 ccttcgtcat ctttaaattt aagagttttt actattgaat aataaaggta tattccagta
     9061 aaaataatct ttaaatacaa gaaaaataaa ctctttgggt atattaaaaa gctaaaaagt
     9121 gtaaatataa aagcaagtag agtacttatc ctgtaaaaga aatctatttg tgtaatgtct
     9181 ttatatttta tcataaacac cgaatataaa atgatgaaaa caattgcgac gattgcatat
     9241 atggtaaata acatattttc aagagtacca tttgaaatta ctatccactt ataccacata
     9301 attggccaaa ataatagtgc taagaactta aaataattat caaacaactt ttctttatac
     9361 attcatcaaa caacctttct taacaaaagc atatatttgt ttttagaatt ttaaataata
     9421 tga
//
LOCUS       DAYAOX010000139         9359 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.139, whole genome shotgun sequence.
ACCESSION   DAYAOX010000139 DAYAOX010000000
VERSION     DAYAOX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.139"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            230..346
                     /locus_tag="ACGI57_002613"
     CDS             230..346
                     /locus_tag="ACGI57_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HFG9717683.1"
                     /translation="MDDDINMVAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            510..1577
                     /locus_tag="ACGI57_002614"
     CDS             510..1577
                     /locus_tag="ACGI57_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9717684.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1769..2299
                     /gene="rnmV"
                     /locus_tag="ACGI57_002615"
     CDS             1769..2299
                     /gene="rnmV"
                     /locus_tag="ACGI57_002615"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFG9717685.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2470..3339
                     /gene="rsmA"
                     /locus_tag="ACGI57_002616"
     CDS             2470..3339
                     /gene="rsmA"
                     /locus_tag="ACGI57_002616"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFG9717686.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3405..4667
                     /locus_tag="ACGI57_002617"
     CDS             3405..4667
                     /locus_tag="ACGI57_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717687.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4818..5933
                     /locus_tag="ACGI57_002618"
     CDS             4818..5933
                     /locus_tag="ACGI57_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9717688.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6119..7015
                     /locus_tag="ACGI57_002619"
     CDS             6119..7015
                     /locus_tag="ACGI57_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFG9717689.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7085..7882
                     /locus_tag="ACGI57_002620"
     CDS             7085..7882
                     /locus_tag="ACGI57_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717690.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7937..9289)
                     /gene="murC"
                     /locus_tag="ACGI57_002621"
     CDS             complement(7937..9289)
                     /gene="murC"
                     /locus_tag="ACGI57_002621"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFG9717691.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
BASE COUNT         3773 a         1015 c         1699 g         2872 t
ORIGIN      
        1 tattaaataa ttataaacta aagtaaataa aatattgagc gattattaaa aagatatata
       61 tataatttaa tttgagcagt ttgttatata ttataaaaaa agagaaaaat aaaaggtaaa
      121 ttgtttctgg ataacttaaa attggaagta agaatattta actagagaag aattatttaa
      181 gtttaggtta gctctaggag tagctgattt tataggaaca atagatttta tggatgatga
      241 tataaacatg gtagcaatta gaccagaaga tataagggta gaatttaata gagatttcaa
      301 agaaattata gacctcaaac tcagaccgta tcgatataaa aactaaagtt agatgatata
      361 gtgttgtaga taggaccatc agctgttatg gcagtattag tgcttttaac aaatataata
      421 gtaaaacttt tgtatgaatt gataataaaa aaattaaaaa ccaagacata aaaatggcaa
      481 caaaaagaag ttgaataagg gaggtaaaaa tgaaatttaa gaagttagtc tctttgggaa
      541 tattatcaac acttttattg agtgtaataa ctggatgtag taatgtttca tcaggttcaa
      601 acttaaagtc taataataag acaaaaggag agttaacagt ttatacagca attgaggaag
      661 atagtataga tacatactta gctacattta aagagaaata tccagatatt aaattgaatg
      721 tacttagatt atcagtagga ggtataacag caagattatt agcagaaaaa gataatccta
      781 aagcagatgt gttatggggt gttgcagcta caagtttgat aattttagat gacaaagaaa
      841 tgctagagcc atacgctccg aaaggctttg ataaggtaaa ttctgcattt aaagatgata
      901 agaaagttcc ttcttgggtt ggaatagatg cttggatgac tggtataaca gtaaatacta
      961 aagaattaaa agctaaaaat atatcagctc ctagttctta tgaagattta ataaagccag
     1021 aatataaaga tcttatatct atgcctagtc cagcttcttc aggaacagga tatttaacag
     1081 tatcagaact catacaaatt atgggtgaag aaaaagcatg gcagtatatg gataaattgc
     1141 ataaaaatat tggtgtatac actcagtctg gttcagctcc agctatatta gcagcttcag
     1201 gagaatatcc cattggtata tcttttggaa atagaggtat aaagttaaaa ggtgaaggtt
     1261 atccagtaga agtagtattt ccaaaagaag gttctggatg ggagatagaa tcaaatgcac
     1321 ttgtgaaaaa agataatata aaagaagcat caaaagtatt tttagattgg gctataagta
     1381 aagatgcaat gaatgagtat tctaagaatt ttgcagttac tacaattgac acgggaaatc
     1441 cggtgccaga aggatttcca aagaatccat tagaacagat gatagataat gatttaaaat
     1501 cagcagcaag aaatagagag gctatactaa ataaatggat agaaaaatat gatggaaaga
     1561 cagaaaaaga gagctaagcc ttgcaataaa atttagtaca aaaagcgcct agaatataag
     1621 ttttatatct aggctctttt gatatattta tttaaaatgc atatttctat ataaaaataa
     1681 atagtttagt accttgatta aattaaataa aaaccttaaa ataaagagat gtaaagaaaa
     1741 actaaaaacg aataaaaggt gaatttatat gataaaagaa ataatagtag tagagggtag
     1801 agatgacgtt acagcagtta agagagcaat agatgcagaa cttataacta caggtggatt
     1861 tggatttcca aaaggtgtaa tggagcgtat aaaagctgct aatgaaagac gtggtgttat
     1921 aatatttaca gacccagatt ttgcaggtga aaaaataaga aaaaaaatag catcagaagt
     1981 tccaggatgt aaacatgcat ttctaccgag agaggaagct aaaaaggatg gagatatagg
     2041 tattgagaat gctactcctc aaagtataat agcagctctg aataaagtta gaactgaaaa
     2101 tacagaaaaa agagatgaat ttaagcaagt tgacttaatt agaaatggtc ttataggtaa
     2161 tgaagaagca tcacttagaa gagatgcact tggaaaaata cttggaattg gatatggaaa
     2221 tgcaaaacaa tttttaaata gattgaataa ttatggagtt gaaagagaag aatttataga
     2281 tgcattaaaa caaatctaaa cgtaatgttt aatgtatgaa aaatatgtat ataaaataaa
     2341 ttgtggaatt acggttaatt taagatgaat tatttataat atacacctta ttttaaatca
     2401 atgtaaatac atataaataa gtataattaa taaaattaaa tatcaaaata agttaaaaag
     2461 gagtaaataa tggatagact atcttcacat aatgcaacaa aggaagtagt acaaaaatat
     2521 aattttaaat tttcaaagtc tttaggtcaa aatttcttga tagatagtaa tataattgat
     2581 aaaatcttat ctggagctag aatcacaaga ggagataata tcatagaagt aggacctggt
     2641 ataggaactc ttactcgtga gatgggtaaa atagcagaaa aagtagtggc tatagaaatc
     2701 gatagaaatt taattcctat attgaaagat accttgtcag atttagataa tacagaggtt
     2761 gtaaatcaag atatactaaa agtagacata caggaattag taaaagataa attaaatggt
     2821 gggccagtta agcttgtagc aaatcttcca tattatataa ctactccaat agttatgaaa
     2881 tttttagagg aagacatacc tgtaacagac atagtagtca tggttcaaaa agaagttgca
     2941 gatagaatga atgctattcc gggtactaaa gattatgggg cattatctat agctgttcaa
     3001 tactattgtg atacagagat tgtggcaaaa gctccaagac atatgtttat accacaacca
     3061 aatgtagatt ccacagttat aggtctacat gtaagagata aaagaaaata tgatgttcat
     3121 aatgaagaca tattttttaa aacagtaaaa gcatcctttg gacaaagaag aaaaactctt
     3181 cttaattcgt tgggaggtct tggttttctt aataaagatg aaataagaga agtattaaaa
     3241 gaagctaata tagacgaaaa aagaaggggt gaaactctta gtatagaaga gttttcagtt
     3301 ctttcaaata taataaatac aaaagtatct tctaaataga aggtactttt tttaattttg
     3361 ttaacatata ttgtttagag aaactgtgtt aaggggggat tgcagtgggt tctaaaagaa
     3421 agtttaaaag agactatata atactagaag ctaaagacat aaactttaga tacaaagaac
     3481 gagtactgcc aaaagctttt gcaaaagttg aagtaaatga tgaaaaagca attattgcat
     3541 tatatgtaga aaatctaaaa tatgttaaag atgggtataa agcagtagca ataaaagatg
     3601 accatggatt aatagactta ggaaatataa ttttaaatga acaaggaaga ggtgaatttg
     3661 ttttagattt agaagattat gatgatgaga taaaaggtat tgcattattg tatgaaagaa
     3721 atataccttt aattggattt aaaggaaata agcttgaaaa ttatgaggac atattatttc
     3781 aagaggtaga ggaagaagaa tatgaagaat atgaagagtt ggaagaagaa gacgaagaga
     3841 tagaggagaa tgaggaaagt gatagagacg aagatagtga agaagaatat attatagaag
     3901 aatatgatga agatgagtat gaagactatt gggaagatga tgaagatatt gaagaaggag
     3961 agttaaaagc aagaacggaa gtagaagatt ctgatgatga ggaaatagaa gagttcaata
     4021 attataagga aatagaaaat tataataggg ttaaagtaca ggaagaatat atagacatag
     4081 aatatgatga agaagaagag gaagaagaag tttttgaagt aaatagagaa gaagataggt
     4141 ttgtttcctc aaaacctact aaatcaaaga aagttcaaaa agatcagaat aatattgaaa
     4201 aaataaaaag agctaatgga gtaagtcaag gagagagtgg aaataaacaa accagtacat
     4261 tacttatgcc aagacaaata aaaaaaggtt tgaaattatt taaagaagta aaaccttttg
     4321 taacagatta tatagataag acaagatggt ggaaaataga gataaatcct acaacactgt
     4381 gtggatatac aatgccttat ttaggatatt taaacgtatt gaattacact atgtatagtg
     4441 atgcagtttt acattcatat aaatacagac actatttatt tggagttcag tatgatgaat
     4501 ataataagag aaaacattat atttatgcta tcccaggaaa tagaaatgaa cagccagaca
     4561 atggaagcac aggattttta agatatcaag cttgtgataa tagaacaaat agtttaggat
     4621 attggctatg ttttatagat tctagacaaa gaaatatagt taggtaaaaa taataaaaat
     4681 gaaaaaattt agactcaatt cttattttac tgaaaaaatt aatgaaatgt agtacaaaac
     4741 tttaaaaact attgataata tttaaatatt acactacaat atatcttgaa atacctatta
     4801 tattatggag gaaaattatg ataaacgagc aaagaatttt aaatgagttt cttgaattag
     4861 tacagataga tagtttatct ttaaaagagg ggaatgttgc taaagtatta gttaaaaaat
     4921 tagaagaaat aggttgttca gtagtaatag acaatgctgg tgaaaaagca aatggagaaa
     4981 ctggtaatat aatagctaca ttaaaaggaa ataaagaagg aaagaaaata ttatttagtt
     5041 cacatatgga tacagtaact ccaggtatag gggtaaaacc tatagtggat gaagcaaatg
     5101 gaataataaa aagtgatggt actacaatat taggttctga tgataaagca ggtatagcag
     5161 ctatattaga aggcttaaga tatataaatg aaaataacat agaacatact gatatacaag
     5221 ttgtattctc tatatgtgag gaatgtggat tagttggagc taaaaattta gactatggaa
     5281 aaatagattc tgaatatgca tttatattag atagtggtgg ttctccaggt gaaataatag
     5341 taaaagctcc tgcacaagat gtaataaatg ttaagatatt aggaaagact gctcatgcag
     5401 gtcttgaacc tgaagctgga ataagtgcta taatggtagc tgccagagca atagaaaata
     5461 tgaatttact tagaatagac gaagaaacaa ctgctaacat aggaattata aacggtggaa
     5521 cagctacaaa tatagtaaca ggagaagtta atatagttgc agaagccaga agtctaaaag
     5581 aaaataaatt agatgttcaa actaaacaca tggtagagac ttttgaaaaa gctgcgaaag
     5641 attttggagc tcaaattgag atagatgtta atagagcata cacaccaata gatgttagtg
     5701 aggattctga aataataaaa cttgcaaaga aggctttttc aaacttaggt atagaaggtc
     5761 atactgaatc tacaggagga ggtagtgata caaatatatt aagtaagaat ggtatcgaag
     5821 ctataacttt aggaattggt atgaaaaatg ctcatacact aagtgaacat atagctataa
     5881 aagacttata tgattctgct aaaatggtag tggagataat aaaggaagca taaatttaaa
     5941 taatagtgta taatatatat atcgcctagc ttaagctagg tgatttttca ttttatagaa
     6001 gattgataat ctctattgaa agcaaagagt atacatttgc atataagaga gtgtttaatt
     6061 tatgttcaaa tgtaggttct taataatatt tagattgttt cataagggag gaataaatat
     6121 ggaaacaagg tatgaagaag caaataaaat aacaatacaa tcaatacttt ggaatatagt
     6181 attaacaata attaaggtta tagctggtgt tattggtaat tctagcgcta tgattgcgga
     6241 tggattacat tcagcatcag atattataag ttctatagga gtactaatag gcaattatgt
     6301 atcttctaga cctggagata gagagcataa ttatggtcat gagaaagcag agacattagt
     6361 atcatttgtg ttatcaatat tgcttatttt cgtttctata acaataggta tagaggcaat
     6421 taaatcttta tttaacttag atgcattaag tgttccatca atattaccat tagtagtatc
     6481 agttatttca atcctgataa aagagtatca atatagaata acaataaaag ttgctaaaaa
     6541 gataaattca ccagccctaa aagctgatgc ttggcatcac aggtcagatg ctttatcttc
     6601 agtagctgct tttataggta taggtggttc tattttaggg tttaaaccat tagacccaat
     6661 tgcgtcagtt gtagtggcta tatttgtagc taaagtagga attagtatac ttataagttc
     6721 tgttaatgaa ttgatggatg tttcagttga tgaagaagag ataaaagaat taaaatttat
     6781 tgtagcagat acagaaggtg ttaaaaatct tggtgatata aagactagaa aacatggagc
     6841 tatggcatat gtagatttga ctatatgtgt ggatgaaaat ttgacagtta agcaaggtca
     6901 tgatattgct accaagcttg aaaaacatat aataaaacat atggaatttg taaaaggtat
     6961 aacagttcac gttgaaccat gtactaattg ccaaggaaat aagtgtaata attaattaaa
     7021 acttaaaaaa aagtgtaaaa agttatataa ttgttttatg attataaact ctaggggtgg
     7081 ttaaatgagc aagaaaagaa aactaaagaa aaaagctgca atattaatag gagtagttgt
     7141 tttctttata atatacttta tatcctttag aataggaata aatatggaaa aggataaaag
     7201 tgaagtgcca aagaaggtag aaaaagaaga agaagataat aggtctttaa ggactcaaat
     7261 aaaagagaag aagaaggttt atatatcaga taaaaatata gaaaacataa aaattgaaga
     7321 agatatgtgg gatgaaataa agttattttt ctcagatttt aagaaagtaa gaaatactac
     7381 ttcttcttac gaagcagttt atgaaggtta ttctgatgat ggagttaaat tttctactga
     7441 ccttaacttt tttagagtat atactgtaaa taaagaagaa tattataaag taccagttag
     7501 ttctaaggat gcttttaaga aacttttaga tgagagtata tatacttcgt ttgacctttt
     7561 aagtcattat aaagattgga aagaggttac tataactcat ggaaaagaaa ttaaaaaagt
     7621 tcatagatgg aaatttgatg atttagcaca taagatgatt tcaaagaggg ttgtaggaaa
     7681 agttcaacct gagaaaagta aggaaagaag tgactataat tttactataa atataaaagg
     7741 tgataatttt gatgtaaaag ttgatacaat gggaaaagat tatgtaaaag ttacttctaa
     7801 gaaaaatgta tcttactatg aggttcatac aagtttattt gattacctta aaaatgagat
     7861 atttgaaatt ggaaagaaat aagactttaa gaaggctatc aaattagata tttgatagcc
     7921 taaattattt tatatattat ttttcaacca acaagtctgc aaccttataa attggaccag
     7981 caccagcagt tattactaaa tcattatcct taacattttc acgtaagtat tttactatat
     8041 cttcaaattc ttttatatag atggcatcta cattgttttg atataatttc tcaaccaagt
     8101 ccttagaatg tatatctcct gggtcttttt ccctagcagc atatatatct gttatgataa
     8161 ctttatcagc agcatagaat gcctctgaaa attcacctaa aagagatttt gttctagtat
     8221 aagtatgtgg ctggaagata caccataaag ttgatttttt taattttttg gcagcagcta
     8281 aagttgcttt taactcagtt ggatgatgtg cataatcatc tactactaaa gcattcttat
     8341 agtacccttt agtttcaaat cttcttccga cacctttata aatttttata ttttttctta
     8401 tagtctctaa gtcaatacca gaaacaagag ctgccattat ggcagaagaa gcattgtata
     8461 tgttatgaag accatataca gaaagttcaa attctcctaa atctcttcct tcatattcta
     8521 ttctaaatat tccatgacca ctattgtcaa agtgaatatc ttttattaca gcatcattgt
     8581 cagaatctct gccgtatttt ataatagtag ctttaacatc atacaatatg tcatcaatat
     8641 tttcatcatc gccatttatt atgaaatagc cttctggggg taataatttt ccaaatttgt
     8701 taaaagaagc cttgatttca tctattccag agaaataatc aagatggtct tcttcaacat
     8761 ttaaaactat agatatcttt gggttaaaat taaggaaact atctacatat tcacatgctt
     8821 cagttataaa gtggttggaa ttacctatct taacattacc acctatcata cttaagttac
     8881 caccaactag tatagttggg tctaagtctg catattcaaa gattgtagat aacatagatg
     8941 ttgtagaagt ttttccatgt gttccagaaa ctgctataga atttttatat tccctcatga
     9001 tttggcctaa aaaagttgct ctgttcatta ccaatttatt tttttcttta gcagccacaa
     9061 gttcttcatt gtctggatga actgctgctg tataaacaac catatttaca tcatctgata
     9121 tattatcttt tttctgacca atataaatag ttgccccttg gtccctaagt ttgtctaata
     9181 ggtatgattc gtttgaatcc gaacctgaaa cttgataacc tttattgata catatttcag
     9241 ctaatgcact catgctgata ccgccaattc ctataaagtg tatattcata ataaaaccac
     9301 ctttcttttt aattaagtcc tacaatatga actatttctg tggtataata atataatgt
//
LOCUS       DAYAOX010000140         9306 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.140, whole genome shotgun sequence.
ACCESSION   DAYAOX010000140 DAYAOX010000000
VERSION     DAYAOX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.140"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            36..1796
                     /gene="prdR"
                     /locus_tag="ACGI57_002622"
     CDS             36..1796
                     /gene="prdR"
                     /locus_tag="ACGI57_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HFG9717692.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            2225..4105
                     /gene="prdA"
                     /locus_tag="ACGI57_002623"
     CDS             2225..4105
                     /gene="prdA"
                     /locus_tag="ACGI57_002623"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050002 - D-proline reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HFG9717693.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            4186..4470
                     /locus_tag="ACGI57_002624"
     CDS             4186..4470
                     /locus_tag="ACGI57_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBO2463/CBO2479 domain-containing protein"
                     /protein_id="HFG9717694.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            4508..5233
                     /gene="prdB"
                     /locus_tag="ACGI57_002625"
     CDS             4508..5233
                     /gene="prdB"
                     /locus_tag="ACGI57_002625"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050002 - D-proline reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:4958..4960,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HFG9717695.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            5313..6071
                     /gene="prdD"
                     /locus_tag="ACGI57_002626"
     CDS             5313..6071
                     /gene="prdD"
                     /locus_tag="ACGI57_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HFG9717696.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            6086..6553
                     /locus_tag="ACGI57_002627"
     CDS             6086..6553
                     /locus_tag="ACGI57_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFG9717697.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            6580..7053
                     /locus_tag="ACGI57_002628"
     CDS             6580..7053
                     /locus_tag="ACGI57_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFG9717698.1"
                     /translation="MGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSEHNE
                     WKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIMGAD
                     GLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK"
     gene            7073..8080
                     /locus_tag="ACGI57_002629"
     CDS             7073..8080
                     /locus_tag="ACGI57_002629"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HFG9717699.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            8200..9081
                     /locus_tag="ACGI57_002630"
     CDS             8200..9081
                     /locus_tag="ACGI57_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFG9717700.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         3642 a         1149 c         1871 g         2644 t
ORIGIN      
        1 gttttgatga ggtactacta taaagggggg actttatgtt ttcgatacca gaagtaaaaa
       61 aagtagaaga ggtaatggat acaaaattta caactataga cgaagacaca agaattgaag
      121 atgcaataaa agaaatgata aaaagcaaca caaaaactct aatggtaata gattcatctg
      181 accaattaaa aggcattata tccatgacgg atatacataa tctttatgag atgcataaaa
      241 agtatgaagg tcaaccagta aaacttataa tgaagaagga tgtaatatat gtcaacgaag
      301 gtttaacctt agatgaatgt agagatataa tgatattaaa aaatataggg attcttcctg
      361 ttcttagaga taataaaatt attggagttc taaaacaaga gcacattagg gactatctat
      421 atatgcattt agaagattat ggattaactt tgaagtatat tataggtcaa attaaagaag
      481 gaatttgtgc tataaataac gaaggtgttg ttatcttgtg gaataagttt atggaggaaa
      541 ggtatgatat aaagtctgaa gatatagttg gtcgaccgat gaatgaattt ttggaaaata
      601 ctatttcaga aaaagtacta aatagtaaag ttggaatgag tgacttgtat tttacagata
      661 aaaaagaaaa tatgtatgca ttagttcatg caaatcctat attttataaa gaagaattta
      721 tcggtgttgt atgtacagaa gttgatgtta cagaagctaa gatattagct cttgaacttg
      781 aaaaagtaaa tgatacatta aaatatctaa agaacgaagt caaaaattta tctaaaggta
      841 gttttgataa gattttaggt aaaagttata aattggaaaa atcgaaagcg attgcaaaac
      901 aagtggctag aacaaatagt agcatattta tttggggcga aagtggtaca ggtaaagaag
      961 tatttgcaag agctattcat gattatagtg agagaaaagg tcaatttata cctgttaact
     1021 gtagtgccat accaaatgag ttatttgaaa gtgagttttt tgggtatgaa tcaggtgcgt
     1081 ttacaggagc aagtaaaaag ggtcgaatag gtatatttga acttgcaaaa gatggaactg
     1141 tatttttaga tgaaattgcc gatttacctc ttagtatgca agcaaagttg cttagagtat
     1201 tacaggaaaa agaaataaga agagttggtg gagataccac aataaaaata aatcctagaa
     1261 taatatcagc taccaataaa gatttgagta aaatggtaaa ggcagaaaaa tttagagaag
     1321 atttatacta tagattaaat gttgttgaaa taaagattcc tccacttaga gagagaaaag
     1381 aagacatagg attattggtt catagctttt tagaggagat atgtaaacaa aataataagc
     1441 ctgttcttac aattagcaaa gatgtaatag atatatttca gaattataga tggaaaggta
     1501 atattagaga attaaaaaat acgattgaaa atattgttgt attgtcacaa aattctaaaa
     1561 tagatgtgga tgatgtacca agttatatga tggattctac aaataatagt actgaagaag
     1621 aagagtatcc tttagattta acgaaggcaa ctcaaaaaat tgaaataaag aatataacca
     1681 aggcacttaa aatgtctaat ggaaataaag ctaaagcagc taagatatta aatataccca
     1741 gaacaacttt gtattataag atagaccaat acaaaataga tgtgtctaaa atatgacaca
     1801 tgactaaatt ttgacaaaat gataaacttt aatttaaaac ctagtaaagc caacgcttat
     1861 ataagtgttg tgtatataga tgtgttaaaa aaaatgaaaa agtgttgatt aattgacact
     1921 ttttttgttt gagaaaatgt ttacaaatac aaaaaataac atattttaaa taaatattta
     1981 tataatattt ggctaaaaat agcgatagat gaagaatcta ttatagaaaa aatctttttg
     2041 ttaataagtt ggcataggaa ttgcttataa tataaagtga ctttaagtga gaaaaaaata
     2101 atattaatca taacaaatta tgtaggtttt aatttactta atttctcagt taaggggtta
     2161 cctgttgagg aattgagatt taaaataata aataagaaaa taatgtaagg aggtatttta
     2221 tactatgtca ataactttag aaacagctca agcccatgca aatgacccag cagtttgttg
     2281 ttgtagattt gaagcgggaa caattatagc gccagaaaac ttagaagatc cagcaatatt
     2341 tgcagactta gaggattctg gattattaac aataccagaa aatggattaa ctataggtca
     2401 agtactagga gctaagttaa aagaaacttt agatgcactt tctccaatga ctacagataa
     2461 cgtagaagga tacaaagcag gagaggctaa agaagaagta gtagaagaaa cagtagaaga
     2521 agcagctcca gtatcagaag cagcagtagt tccagtaagc acaggagttg caggtgaaac
     2581 agttaaaata cacataggtg aaggtaagaa cataagctta gagatacctt tatcagtagc
     2641 tggtcaagca ggagttgctg ctccagtagc aaacgttgct gctccagtgg caagtgcagc
     2701 agcagaagta gctccaaaag ttgaagaaaa gaaactttta agaagcttaa ctaaaaaaca
     2761 ctttaaaata gataaagttg aatttgctga tgaaactaaa atagaaggaa ctactttata
     2821 catcagaaac gcagaagaaa tatgtaaaga agctaatgaa actcaagagt tagttgtaga
     2881 tatgaagtta gaaataataa ctcctgataa atatgaaact tacagtgaag ctgtattaga
     2941 tatacaacca atcgctacta aagaagaagg cgaattaggt tcaggtataa ctagagttat
     3001 agatggagct gtaatggtat taactggtac agatgaagat ggagttcaaa taggtgaatt
     3061 tggttcttca gaaggtgagt taaatactac tataatgtgg ggtagaccag gtgctgctga
     3121 caaaggtgaa atattcatca aaggtcaagt aacaataaaa gcaggaacta acatggaaag
     3181 accaggacct ttagctgctc accgtgcatt tgactatgta actcaagaaa taagagaagc
     3241 attaaagaaa gttgacaact ctttagtagt tgatgaagaa gtaatagagc aatacagaag
     3301 agaaggtaaa aagaaagttg ttgttataaa agaaataatg ggacaaggtg caatgcatga
     3361 taacctaata ttaccagttg agccagttgg tacattagga gctcaaccaa acgttgactt
     3421 aggaaacatg ccagttgtat tatctccact tgaagtatta gatggtggta tccatgcatt
     3481 aacttgtata ggacctgcat caaaagaaat gtcaagacat tactggagag agccattagt
     3541 aataagagct atggaagacg aagaaataga tttagtaggt gttgtatttg ttggttctcc
     3601 acaagtaaat gctgagaaat tctatgtatc taagagatta ggtatgttag ttgaagctat
     3661 ggaagttgat ggagctgtag taactactga aggtttcgga aacaaccata tagatttcgc
     3721 atctcacata gagcaaatag gtatgagagg tataccagta gttggtgtaa gtttctcagc
     3781 tgttcaaggt gctctagttg ttggtaataa atacatgact cacatggtag acaacaataa
     3841 gtctaagcaa ggtatagaga atgaaatatt atctaacaac actttagctc cagaagatgc
     3901 tgttagaata atggctatgc ttaaaaatgc tatagaaggt gtagaagtta aagctcctga
     3961 aagaaaatgg aatccaaatg ttaaattaaa taacatagaa gctatagaaa aagttacagg
     4021 agaaaaaata gtattagaag agaatgagca atctctacca atgagtaaga agagaagaga
     4081 aatatacgaa aaagacgaaa actaaaattt agatactaac taagatttag acaataacat
     4141 aaatttagat ccaagtttaa aacagtatag agaggtagaa ttataatgga taggttaaaa
     4201 tatatctcat cagagacttt ttatgaaggt attatagttg acatcaaagg cggtggagtg
     4261 actatagacc taaaaggtag acttggacaa tttaaaatac ctaatagaat gcttataact
     4321 gactacgaac ttaagatagg tcaagaagtt ggattcatgc taagctatcc tgaggtatta
     4381 agtccagagc ctaatcaaga gtatgtagaa aatattagga gagaaaaaga aaaacaagca
     4441 gaaatgcaat caaaacaaga agaaaaataa taaataatcg tttgaaaata gaagggagag
     4501 gaaatatatg agccttacaa cagtacaagg acttcaatct gaaatattcg ttccaataac
     4561 acctcctcca gtttggactc ctgtaactaa agaattaaaa gatatgacta tagctttagc
     4621 tacagcagct ggtgttcatt taaaatctga caagagattt aatctagcag gagatactac
     4681 atttagagct atacctaata cagctacagt tgatgaaatg atggtatcac atggtggata
     4741 tgacaatgga gatgtaaaca aagatataaa ctgtatgttc cctatagata gattacatga
     4801 attagctgca gaaggattta tcaaaggagt agctcctatg cactatgcat tcatgggtgg
     4861 tggaggaaac caacatgtct tcactgaaga aactggtcct gctatcgctg ctaaacttaa
     4921 ggaagaggga gtagacggtg tagttatgac agctggctga ggtacttgcc atagaactgc
     4981 cgtgatcgtg cagagagcaa tagaggaagc tggaatacct acaataataa tagcagctct
     5041 tcctccagta gttagacaaa acggaactcc tagagcagtt gctccattag ttccaatggg
     5101 tgctaatgct ggtgaaccac ataatataga aatgcaaaca catatattaa gagatacatt
     5161 agagcaatta gttgcaatac catctgctgg taagatagtt ccattaccat acgaatataa
     5221 agctcacgtt taaaatgtaa tttatatttg aaaatataga acccttctcc tacacggtta
     5281 ggagaggggt tttaagaaca aaggtggata ttatggaaga aaaaataatg agaagacttg
     5341 ttataaaacc gtttcatatg aatgaagtta atttcggttc aaaaacttct ataaagaaag
     5401 atgtacttac aatagattta tcaagtatag atgaaataaa agaaagagaa gagttaataa
     5461 cagacataaa agtagacatt ataaaacctg gagattatga cagagaaatt aatactatta
     5521 tggacattat accaatatca acaaaagttt taggcagact gggagagggt attacacata
     5581 ctttaacggg tgtatatgta atgttaactg gtgcagatga agatggaaat caaatgcatg
     5641 aatttggttc ttcagaagga attttgaaag accaaatggt atttgggaga tatggaactc
     5701 catcagttga agattatata atccatgtag atgtaacttt aaagggaggt ttaccttttg
     5761 aaagaacact tccacttgca gcattcagag catgtgatga ttttatacaa gaaataagag
     5821 cgtctttgaa aatggaagac ggaagaaatg ctactcaagt tcgtgaatat tttgacaaaa
     5881 ttcgaccaaa tgcaaaaaag gttgtcatag taaaacaaat agcaggtcaa ggtgctatgt
     5941 atgacaatca gttattctca aaggaaccta gtggatttga aggaggaaca tcaattatag
     6001 atatgggaaa tgtacctatg attatatctc ctaatgaata cagagatggt gcccttagag
     6061 ctatgactta agagaggtgg atattatggg aataggacct tcaacaaaag aaacatctct
     6121 tcatcacttt agagacccac ttttagacat agttgaaagt gataaggatg tagatttact
     6181 tggagtaata gtagtaggaa ctccagatgg aaatgaaaat aaaacctttg tgggacaaag
     6241 aacggccgct tggctggaag ccatgagagt agatggagct atagtttcat cagatggttg
     6301 gggaaactca catgttgact acgctaacac atttgaggaa ataggaaaaa gagatatacc
     6361 tgtagttgga gttacattta atgggactca agctaagttt gttgtaagca atcaatatat
     6421 ggataccata gttgatatga acaaatcaaa agaaggtatt gagacagaag tagttggaga
     6481 aaacaacaca aatgaaatag atgctaaaaa agctttggca tttttaaaac ttaaaatgag
     6541 aaaacatgga taaaaaattt aagtaaggga atgagttttg tgggaatttc tacatcaatg
     6601 aaaaacacat cacttcatat atttagagac ccgttactag atttgataga taatgattct
     6661 gacatagact tgttaggagt ggttgtagtt gggacatcag agcacaatga atggaaaaca
     6721 tttttagcaa ctagacttgg aagatggatt gaatcattgc gacctgatgg agtgattata
     6781 actttagata tggcaggaaa ccagcatatt gattttacaa atgctatagc agagtttgta
     6841 aaatcagata tacctacagt aggtcttact attatgggag cagatggatt agtaattacc
     6901 aatccatatt tggataaagc taccataatt gattataaaa agacaacagg ctacatagaa
     6961 acagaagtag ttggagacaa tcacatggat gaagttgatg ttaaaaaagc attggctttc
     7021 ctaaaattaa aaatgcgtaa agacgcaaaa taaacctaag ggggaatttg atatgaaatt
     7081 tagcagaagt atacaagcta tagactctca tacagcaggt gaagcgacta gaatagttgt
     7141 aggtggaata cctaacataa aaggaaattc tatgcctgag aaaaaagaat atttagagga
     7201 aaatctagat tatttaagaa ctgctataat gttagagcca agaggacata atgatatgtt
     7261 tggttctgta atgactcaac cttgttgccc agatgctgac tttggaataa tcttcatgga
     7321 tggtggcgga taccttaaca tgtgtggcca tggtacaata ggagctatga cagcagctat
     7381 agaaacaggt gtagttccag cagtagagcc tgtaactcat gtagttatgg aagctccagc
     7441 aggaataata agaggagacg ttacagttgt agatggaaaa gctaaagaag tatcattctt
     7501 aaacgtacca gctttcttat ataaagaagg tgttgaagtt gacttaccag gtgttggaac
     7561 tgttaaattt gacatatcat ttggtggaag cttctttgct ataatacatg caagtcaatt
     7621 aggtttaaaa atcgaacctc aaaatgctgg taaattaact gaactagcta tgaaacttag
     7681 agatataata aatgagaaaa tagaaataca acatccaact ttagctcata taaaaactgt
     7741 agacttagtt gaaatatatg atgagccaac tcatccagaa gctacttaca aaaatgtagt
     7801 tatatttggt caaggtcaag ttgacagatc tccatgtgga actggaacaa gtgctaaatt
     7861 agctacttta catgctaaag gtgaattaaa agtaggagaa aaattcgtat atgaaagtat
     7921 attaggaact ttattcaaag gtgaaatagt tgaagaaact aaagttgcag atttcaatgc
     7981 tgtagtacct aaaataactg gttctgctta tataactgga tttaatcatt ttgtaataga
     8041 tgaagaagac ccacttaaac atggatttat tcttaaataa gtaataaata aattaggaaa
     8101 ttaaaataga atagtagcca cttgtcggtt gctattctat agcttttagg ctataagatt
     8161 cagaatatat gaaaaaataa aaaaatagga ggatggatta tggtagcaat cttaaaggca
     8221 ttatatgtag tattcgcagc aatctttgca gttgtgtttg gtaaagacgt agctaaagca
     8281 aacaaagagg ggaaactaga agaacaaagt actccaaaag ttgctgtaac aggatttata
     8341 acagacttct ttgatacatt aggaataggg tcatttgctc caacaatagc aatgtcaaag
     8401 gctttaaagt taaatatacc agataaaaaa atgcctggta cattaaacgt agcacatact
     8461 attccagttg ttacagaagc attcatattt acatcaatta tcccagtaga tggtgttact
     8521 ttagtatcta tggttgtggc agcagcagtt ggttcatata taggtgcagg aataatagct
     8581 aagatggacg agagaaaaat acaacttgtt atgggtgtaa ctttagcatt aacagcaata
     8641 ttaatgttat taggtcatcc ttggattaac gtattaccag gtggaggaaa tgaattaggt
     8701 cttactggtg ctaaattagt aataggtgta ataggtaact ttatactagg agctttaatg
     8761 acagctggta taggtctata tgctccaggt atggcaatgg tttatttctt aggaatgtct
     8821 gcaaaagttg cattcccaat agttatgggt tcttgtgcat tattaatgcc agttgcaagt
     8881 atgaaattca taaaagaaga tgcatatact aaaaaagcat cagttataat agctatatgt
     8941 ggtttaatag gagttttcgt agctgcatat atagtaaaaa ctctaccaat ggatatatta
     9001 aaagcattag taatagtagt tatagcttat acttctgcta caatgataat ggctgcaaat
     9061 aaaaacaaaa agactgctta gttaaagact acttagtgtt tattaaatat tacagctaat
     9121 aataaagatt ataaactgct ttaaaatatt gaattaatat tttagggcag ttttttattt
     9181 actaaattaa tataaagcaa ttaacatgcc aattatgtaa tatatatagt gatttagtag
     9241 atttaatcta aaaaacgttg tatttttaaa gaataaagca taatttaaag ctaaatattt
     9301 aaattt
//
LOCUS       DAYAOX010000141         9210 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.141, whole genome shotgun sequence.
ACCESSION   DAYAOX010000141 DAYAOX010000000
VERSION     DAYAOX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9210
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.141"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            82..1878
                     /locus_tag="ACGI57_002631"
     CDS             82..1878
                     /locus_tag="ACGI57_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HFG9717701.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENIILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(2018..3799)
                     /locus_tag="ACGI57_002632"
     CDS             complement(2018..3799)
                     /locus_tag="ACGI57_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HFG9717702.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(3822..5708)
                     /locus_tag="ACGI57_002633"
     CDS             complement(3822..5708)
                     /locus_tag="ACGI57_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HFG9717703.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(5725..6222)
                     /locus_tag="ACGI57_002634"
     CDS             complement(5725..6222)
                     /locus_tag="ACGI57_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HFG9717704.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(6540..8021)
                     /gene="cls"
                     /locus_tag="ACGI57_002635"
     CDS             complement(6540..8021)
                     /gene="cls"
                     /locus_tag="ACGI57_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFG9717705.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            8339..9109
                     /locus_tag="ACGI57_002636"
     CDS             8339..9109
                     /locus_tag="ACGI57_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFG9717706.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         3191 a         1476 c         1350 g         3193 t
ORIGIN      
        1 aagtacagtg ttagattata ataatactag attaatttta tatttgagtt tcattatata
       61 acaagaaagt tggaattttt tatgggaata gaatatttta gtaaactttt agaaagtagt
      121 actgaaaaag aattgctctt aagtaaaaag tcaaataaca taagtacagc aagattaata
      181 tcttttttga tagtgatagc agggcttgct attggatttt ataataaaaa tatagtaggt
      241 atttttgttg gagttttatc aataataata tttatagcac tcttagttat tcatagtaaa
      301 gtaaaagaag aagaaacata ttttaaaagt aagagcgaag tattaaataa gtatattaag
      361 agatttggag atggttggaa ggaatttaaa atagatggta aagaatactt aaaagatgaa
      421 aattcgcaag caaaagattt agacttattt ggaagagcat cattgtatca atatatctgt
      481 gtagctaata catcttatgg gaaaaatttt ttagccaaat atttgtggaa tgaaaatcca
      541 gatgagaata taattttaga aagacagaga gctataaaag aacttttatc aaaagatgat
      601 ttttctattc atattcaaac actcagtaat ataattggta aagagcaaaa aaacaattca
      661 gatagtagta tagaatcgtt tatagaatat ggagaaaata agaatgtgta tataccaaaa
      721 tggatgcata tttttacttg gggtttacca actgcaacaa tcttatcttt tatattttgt
      781 atgttaggat ttttacctgt tttacctgtt tttttacttt ttataatcca gttagggttt
      841 agtggttttg gatatccaag acttatgcaa actcttgctc cattattttc atttagtaga
      901 agtatacaag tatatgaaaa aatgtttgag gttttagaaa aagagacgtt tgagagtgca
      961 tatttgaaag aattacaaga taaactttct aagggaagtg gagtttcaag aggtgttaaa
     1021 caattaaatt caataggaaa tgctgtaaac ctaaggtata atcagatact atatataatt
     1081 gcctgtggtg ttttgatgtg gaactatcat tgtgcagaag ctttggagcg atggaaaggt
     1141 atatatggaa atcaaattag agattggttt gaatctattg gagaatttga agctctaata
     1201 agtttaacag ttgtttctca tgtaaaggaa aatacatgtt ttccagtaat aaaatatgaa
     1261 gatactccaa gattgaaagt tgaagaggta taccacccat taatagcaga aaaaagtgtc
     1321 atagcaaatt cgatacaatt aaattctcag acttgtatta tcacaggttc aaatatgtct
     1381 ggaaagacaa catttttaag aagtatcggt gtaaacttag tgctggcata tgcaggtgct
     1441 ccagtatgcg ctaagagttt tgatgcgacc tgtatggcta tatttacttc tatgagaata
     1501 caagatgatg taagtcaagg aatatcaact ttctatgctg agattcttag aataaaatca
     1561 atgattcaat atagtttaaa agaactgcca atgttggttc tagtagatga aatttttaag
     1621 ggaactaact ctgctgatag aattattgga gcaagtgagg cagtaaagaa attatcaaaa
     1681 ccctgggtta taagtatggt gacaacacat gactttgagc tatgtgacct ttcaggtagc
     1741 ggagatgttg aagttgtaaa ctatcatttc tcagagtact atgtagacga taaaattcat
     1801 tttgactata agataaaaga tggaagatgt aaaactacaa atgcaaaaca acttatgaga
     1861 atggcaggta ttctttaatt aatagatagg ttaattaata aataagtcta tttagaaata
     1921 taatttaatt tgtgaaatta ataaggattg tcttaaatta cgtaaaagta ttttaagaca
     1981 atcctttttt aagaataaat tttatacttt gttatgacta cttagcttct tcacaagcac
     2041 attcttgtgg acactctcca tagacacaac tcttatcaaa gtaatgagta tgaaggtatt
     2101 tatgagctac atcactattt ggttctccaa agaagtcttt ataaatagct tgtaaatcag
     2161 gattctcatg agatttacga atagccttat ttctatctat ttcatataac gcatttgcac
     2221 gtctatcaac tatttcagta ttaccatggt gataaggttg accagcacct ccaacacatc
     2281 caccaggaca tgccataact tctatgatat ggtatttaca ttctccactt ctgaccttat
     2341 ccataatctt tctagcgttt ttaagtgtat ttacaataca tacattaact gtagttccat
     2401 caacatctat acttgcttcc ctaattcctt taagacctct aacattagtg aagtttacat
     2461 tatctaaagt cttattagtt atcttctcat atgaagtacg tagggcagct tctaaaactc
     2521 caccacttgc tccaaatata gatgcagcac ctgtagattt tcctagagga ttatcaaaat
     2581 cttggtcttg aagattaggt aaatcgattg cagcttcttt tatcatctta gctaactctc
     2641 tagtagttat agaaaggtca acatctagta ttccagattc acttaattct tctcttgaag
     2701 cttcatattt tttagctaca caaggcatta cagatacaac ataaagttct tcaggattga
     2761 ttcctaatat tttaggtgca tagtagtttt tagctataga accaaacata ccttgaggag
     2821 acttacagct agatgctaag tttaacttgt ctggataatt gtgctctaag aagtttaccc
     2881 atgcaggaca acaacttgtt aatataggaa gattttcacc tttttggatt ctttctataa
     2941 attcagtagc ttcttccatt atagtaaaat ctgcaccaaa gtttgtatca aatacatgtt
     3001 caaatccaag tgcttttaaa gcagctacca ttttccctgt agagattgac cctggttcta
     3061 atccaaactc ttcaccaata gcaactctta cagcaggagc aacttgcact actatagtct
     3121 tttctttttt atttaaaaca tcccaaagtt taggtacatt atctacttct gttaatgctc
     3181 cagttggaca gacagatata cattgaccac agaatgtaca ctctgtttca accatatcag
     3241 agttaaagaa tgtactaact aaagtattaa atccacgatt aactccagat aatgctccta
     3301 ctgtttgtat gtcattacat acagtctcac atcttctaca taatatacac ttagaatggt
     3361 ctttaactaa agatttagta gaagtatctt tacctgcaaa agatttagaa ccttgatatc
     3421 ttatttttct tactcctaaa tcagcagcta tagtctgtaa ttcacaatct ccattttttt
     3481 cacagacaaa acaatcttgt ggatggtctg ataacaataa ttcaacaata gttctacgag
     3541 catttaatgc tttagcacta ttagtctgaa ctttcatacc ttcctttata actgttccac
     3601 aagctggaac taatcctctc tctgtctcaa ccatacaaac acgacaagaa gcacaagtgt
     3661 caagcttatc tatttcattc atatgtaaat gacaaaggtt gtgtattttt atatttatta
     3721 atttcgcagc atctaagata gaagttccac taggtgctga aacgtgtttg ccattaatag
     3781 ttaaattaac taaactcatt gttattcctc ctccaaataa gttatttctt gattatagcg
     3841 ttaaatgtac aattatctat acaagcacca cattttagac acttagaagt atctattgta
     3901 tgttgttcct taacagaacc tgttatacaa ccagcaggac acactctaga acattttgta
     3961 caacccttac agtcatctgt tataaagtaa gaaagtaagt tctgacattt accagcagga
     4021 catttcttat ctacaacatg agctatatac tcatctttga aatattgaag agtacttaaa
     4081 acagggtttg gagcagcttt accaagacca cataatgaag cagtttgtat agtttcagca
     4141 aggttttcta ggtcatctaa atcttgtaga gtaccattac cttctgtaat tttagttaat
     4201 atttctaata atcttgtagt acctatacga caaggtgtac atttaccaca agattcttca
     4261 acagtaaact caaggaagaa cttggctata tctaccatac agtcagattc atcaagaaca
     4321 agcataccac cagagcccat cattgaaccg atagaactta atgaaccaaa gtctatagga
     4381 gtgtctaaat gttcagttgg tatacatcca ccagatggtc cacctgtttg aacagcctta
     4441 aatttcttac cttcagggat tcccccacca atttcaaata caatatctct taaagtagta
     4501 cccattggaa cttcaacaag tcctacattt tctacctttc caccaagagc aaagacttta
     4561 gtaccactag aatcttcagt acctaagttg gcaaaccaat ctccaccctt aagtataatt
     4621 gcaggtatgt tagcaaaagt ctcaacgtta tttaagcaag taggagattt ccatagacca
     4681 ctcttagaag aactataagt tttcattcta ggttcaccac gtctaccttc aattgaatgc
     4741 attagcgctg taccttcacc acaaacgaaa gcacctgcac catattttaa ttctaattta
     4801 aagttaaatc cagtgcctaa gatattttcg ccaagtaaac cgtatttttc agcttgagct
     4861 atggctactt ttaatctttc tatagatttt ggatattctg ctcttatgta tatgtatcct
     4921 gtatcagaac ctatagcgta tccacatatt gccatagctt ctagtacact atgagggtca
     4981 ccttcaagta cagatctatc cataaatgca cctgggtcac cttcatcagc attacataca
     5041 acaaactttt tatctgttgg accagctgga ttttttgaag cagcttccca tttagagcca
     5101 gtagggaaac ctgcaccacc tctaccacga aggccagaag tttttacctc tgcaataact
     5161 tcttgaggag taagagaagt taaacatttg cctagagcta aataaccatc attggcaata
     5221 tattcttcta tactatctgg gtctataaga ccacaatttt ttaaagctat acgtaactgt
     5281 tttttgtaga aagacatttc ttcttgttct tcaacttttt tacttaaaga ttcttcctca
     5341 tataataatt cctcgacaac tgtatttcta attaagtggc tttgaactat cttttcagca
     5401 tcagatggct caacttttac gtagaaaacg ttatcaggat aaacttttac aataggacct
     5461 tgagcacaga aaccaaaaca acctgtcaaa cgaacactaa ctttatcttg gattcctgat
     5521 ttctttattt cattttctaa ttcactaact atttctagac tgtttgaaga tgtacatcca
     5581 gtatcacaac aaacaagtaa ttctcttctt aatacatttt ccttagaata attttttctt
     5641 aagctaagat taggtttcag ctcatcagct aaaactttta attcatcaaa tgaatttact
     5701 ttacgcatta tttattcacc cctcttaaca attcaattct aattctctat aagtatctaa
     5761 tatttcgctt acttggtcag gtttcacttt accataaact ttaccattta cagtaacaac
     5821 tggagctaaa ccacatgccc ccaaacatct taaaccttct aaagaaaatt taaaatcact
     5881 agtagtttct ccaactttta tgccaagctg tttttcaaaa gcacttaaga ttttgtcagc
     5941 accttttaca aaacatacag ttcctagaca gacactgatt ttatacttac ctacaggttc
     6001 tgtagtaaaa taagaataga atgtaacaac cccatatacc tttgcaggag ctactccaag
     6061 ttttctagca acatgaagtt gaacttcttt tggaagatat ccaaatatac cttgggcttc
     6121 atgtagaact tgtattaacg caccttcctt tgttgcaaga gagtcaataa atatatctag
     6181 ttcgtcaaat aattgcttgt tgtgtgaaat aaaatcgcac ataacaaatc ccccttttct
     6241 ttttaaagtt attatttttc ataaaataat taaccatgag ttgaccccaa cattatgtgg
     6301 gtgaacaaaa ccaaaacaat aaagaaagaa taatatacaa caatttcttt atttataaaa
     6361 acaatgcaaa aaagttacct attaaaaata aaaactatac tagtctatta taatataaaa
     6421 taattcagaa tagttaaaaa aaataaaaaa aatgagctaa attcaataaa aaaaaaataa
     6481 tacatataaa aaactttaaa aaataaacca aaatgtaaaa tttttataaa ataaaagatt
     6541 tataaaactg ctgataatag tcttgaaatt gattccttga tttttatata agtagaccta
     6601 gaattatata tatctaatgt tatttcctta gaatttaaaa tatcattttc aaatataatt
     6661 ctttgttcca atgctttttt tgaagagtac ataaatgcat taacttcgaa attaagctca
     6721 aaacttctga tatccatatt agctgttcct atagagcata tagagtcatc tataactatt
     6781 gtcttagcat gtaaaaatgc attttgacca taagtataga ttttagctcc aaactttaaa
     6841 agttctccag catatgaata tgatgcccag tatacaaagg gatggtcagg tttagaagga
     6901 atcatcactc tcacatcaac accagataaa caggctattt ttaaagtatc tataaaagtt
     6961 gagtcaagta ttaaataagg actctgtatg tatatatact ttctagcttt ctgaatcatt
     7021 ttaaggtaac catatttaat ctcatcaagt tctgatatat ctggaccact tgatactatc
     7081 tgaatcccaa tattttcaga aggatgatta gaatttgtat cttcttctat aaagtatttt
     7141 tctaaatcta agtcttcttt agttgtatat ctccaatcaa ggatgaatct catatttagg
     7201 tcaattacgc aatcaccagt aagcttagta tgtgtatctc tccatagacc aaattttgga
     7261 tttctaccta aatattcatc accaacatta tatccaccta caaatcctac cttaccatca
     7321 atgacaacaa tttttcggtg atttctatag tttaagttaa agtttacaat tttcaagaat
     7381 gatgggaaga agcttccaac tttaacacca ctttctttaa gtctggaaag tgtactattt
     7441 tttagtgttc taccaccaac tgcatcaaat aaaagtctta cttcaacacc ttctttagct
     7501 ttgtctataa gaatgtctat tatttcagtt ccgattttgt catctttgaa aatgtaaaat
     7561 tgtatgttta tatatttttt agctttttta agttcttcaa gaagactgtt aaaaaattga
     7621 ctagattcag cataaatcca cacatcgtta ttattggtat aatgagcatt atttgaatta
     7681 gccaatgcat aaatcatatc cttatgttga tgtatatctg aatcaatttc agatgtagat
     7741 tggagtttaa cctgagtatc tagtatatta cttttgatta tcttatcatc cttttctttc
     7801 aatctaaaca tattgttttt tgaaatattt ctgccaaaag ctatataaag tatgaatcca
     7861 agagcaggaa tcaatactaa tataagaagc catgttaaag ttgtatttat gtttcttttt
     7921 tctctaaaga taaggtttag tataaccatg aaattaatta tatatattgt agtaactgct
     7981 atttcatata ttgacatttc gaaaaatgat atatcaaaca taaaattccc tccttcaaat
     8041 atttattaaa ataagtaatt atatataata acacaaacat atatctatta tatataatac
     8101 ctcaataatt aaaaagtaaa ataaataatc aaaaggtaaa tttttcaaat tcaatataaa
     8161 ttatttcgaa attaacaaag ttgttatata ataaatagta taattatatg agacaagtat
     8221 atataagtat atagtatatt ttttatgtta ataaaattta ttatatatca tattgttatt
     8281 atataattat tgccataaaa agacaaggga aagtataatt tttaaggggg aaggatttat
     8341 gagtaaagga ttcttgacac ctggggaaac tgcacaagca actataagtg caggcattaa
     8401 aaaagcaaat atttcgacac aaaatgcaat tctattaggt ttatttggag gagcatttat
     8461 aggttttggg gcattgggga gtatgatagt tagtcaaact tttggcaaga tagacccagg
     8521 ggtagctagc tttttaagtg ctatggtctt tccagtaggt cttatgcttg tagtagtggc
     8581 aggagcagaa ttattcactg gaaacacttt aatggctatt gcacttatgg acaaaaaaat
     8641 aacattagga aaaatgttaa gaaactgggg actggtttat tttgcaaatt tagtaggttc
     8701 aattttgcta gttgcacttg tgttttattc ttctacattg acaggagatg cagctacaaa
     8761 agctatagct gtggcagaag ctaaggttaa ttcaactata gcatcaatgt ttttaaaagc
     8821 aatactttgt aatattctag tcgttttagc tgtatggatg gctacagcat ctcaagatat
     8881 aatgtcaaaa ttggcttcat gttggacagt aataatgcta tttgtattat gtggattcca
     8941 acatagtgtt gcaaacatgt tttttatacc aatgggaatg gcattaggag caaatataac
     9001 tatggttcaa tttataacta atttggtatt tgtaacactt ggaaatatcg ttggaggttc
     9061 catagtagtt ggagggattt attatctttg ctatgtcaaa aatagttaga taaataatac
     9121 gaattaaatt gccttttaaa gcattgatat cagtgatatt gatgcttttt ttaaattatt
     9181 tatattcttt tatatgttgt tatttttttt
//
LOCUS       DAYAOX010000142         9132 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.142, whole genome shotgun sequence.
ACCESSION   DAYAOX010000142 DAYAOX010000000
VERSION     DAYAOX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.142"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            128..2500
                     /locus_tag="ACGI57_002637"
     CDS             128..2500
                     /locus_tag="ACGI57_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HFG9717707.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            2515..3417
                     /locus_tag="ACGI57_002638"
     CDS             2515..3417
                     /locus_tag="ACGI57_002638"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFG9717708.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            3437..3988
                     /locus_tag="ACGI57_002639"
     CDS             3437..3988
                     /locus_tag="ACGI57_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9717709.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            4152..5024
                     /locus_tag="ACGI57_002640"
     CDS             4152..5024
                     /locus_tag="ACGI57_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9717710.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANSKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            5188..6168
                     /locus_tag="ACGI57_002641"
     CDS             5188..6168
                     /locus_tag="ACGI57_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717711.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(6666..8711)
                     /gene="bclA1"
                     /locus_tag="ACGI57_002642"
     CDS             complement(6666..8711)
                     /gene="bclA1"
                     /locus_tag="ACGI57_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HFG9717712.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGNTGVTGSIGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGPTGATGPTGEI
                     GPTGATGATGVTGSIGPTGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTG
                     ATGEIGPTGEIGPTGATGPTGVTGSIGPTGATGPTGATGEIGPTGATGPTGNTGVTGE
                     IGPTGATGPTGNTGVTGEIGPTGATGPTGVTGEIGPTGNTGATGSIGPTGVTGPTGAT
                     GSIGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIG
                     NSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTT
                     EPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT         3256 a         1207 c         1584 g         3085 t
ORIGIN      
        1 tgttcaaggt agatgaaaaa aaataagtta ttttgcttcc attttatgct aaaggacagt
       61 ataatataat taaagtatgt tataacatac aaaaatgtag tatatattat ccaaaggggg
      121 attttaaatg agtcaaacta atagaataga agcatttaga caagaatata ttaattctaa
      181 acctatgata tgttgtgaaa gggctagaat ttttacagaa tcacataaaa aaactgaggg
      241 agaagctatc tgtataagaa gagctaaagc ttttttggag acatgtaaag aacttcctat
      301 aaaaatcttt gaaaatgaat taatagtagg tacagcagga aaatttagaa gaacagggat
      361 attgacacca gagttttctt ggcaatgggt agacaaagaa atggatactt ttgataaaag
      421 aactcaagac ccatatgtaa tatcaaaaga acaaatagaa tttataagaa aagaaatatt
      481 tccatattgg aagggaaaat ctttggaaga agttttctta gcaagaatcc cagaagatac
      541 agctaagata ttagtagata caggaataat agataatgat tctaagtgga gacaggcagt
      601 tggtgaagta acaccagatt atcaagatat attgtttgta aaaggatata aagggattaa
      661 ggaagatgca gataaaaaaa ttaaagaatt agatatatca gtttcagaaa atattgaaaa
      721 gatagatttt tacaaatctg tttctatagt tgcacagggt attatgacat tggcacaaag
      781 atattcaaat cttgcaaaag agatgagtaa gcaagaaaca gatgaaaaga gaaagttaga
      841 acttataaaa atttcagaga tatgtatgaa tgtaccagca aaccctccaa ctaattttta
      901 tgaagcaata caatttgtat ggtttgttca attaggtggt attctatcag aaaatccatt
      961 ggcattaaat ttaggtagat ttgatcaata tatgtatcca tattatgaaa atgatgctag
     1021 agaaggtaaa ataactgaat cagaagctca agaattgata gaagcacttt ggattaagtt
     1081 atcagaatgg gtatggacta tatcagcaaa tacagcaaat tactttgcag gatataatca
     1141 attccaaaat cttactgttg gtggtaaaaa gagaaatggt actgatggaa cgaatgatat
     1201 ctcatatatg tgtttaaagg ctacagaaag tgtaaaaact caccaaccag gattaagtgt
     1261 tagagtgagt caaggagcac cagacaattt cgtaatggca gttgctaaat tggtaaaaca
     1321 aggaactggt ttccctgcta tacacagcga tagtgcaggg gcacaaatgt tattacaaga
     1381 tggatatgat gcagaagatg ctagagattg gagtaactgt ggttgtgtag ttcctcattt
     1441 tagaaaaaca ggacagtgga cttcagccgt aaatatcaat tttgcagcag ctttagaata
     1501 tgctatgaat gaaggtaaga gtagattaac tggagaaaaa atggggttag atacaaaaaa
     1561 tatcacagaa tttacttctt ttgaagaact taaggatgag tttttaaagc aattagcata
     1621 tcttgtaaaa agttctgtta taggaactac tgttgctcaa caaattcata aagaaatggt
     1681 accaagacca tttttatcta cttgtgtaga tggatgtttg gataagggag ttgatttaag
     1741 taaaggagga gcaaaatata atataggtcc tgtattaact ggtatagggt taggtgtagt
     1801 ttcaaattca ttggcagcta taaaaaaatt agtatttgag gataaggtga ctacgttaga
     1861 agaattaaca aaagcactaa ataatgattg ggaaggttat gaagaattaa gaaaacttgc
     1921 gttggatgtt cctaagtatg gaaatgataa tgattatgta gattcattag caattgaagt
     1981 ttctgatttt tattatactg aaactagaaa atacaaggat atttttggct ctaaatttaa
     2041 tagtgcattt atgggtatat caaactatgt accaactgga aaaatcgtag gcgcaactcc
     2101 ttgtggaaga aaagcgacaa aacctttgac agagggagtt tctccatttg ttggtactga
     2161 tacaacaagt ccactagcag caatgaaatc tgcatcaaaa ataaatcatg atgttcatac
     2221 tggtggaaca cttctaaatt taagactgaa tcaagatttg gtggaaactg aaagaggact
     2281 tagaaatcta acatctatga ttaaatcata ttttgcttta ggaggattcc atgtacaatt
     2341 taatacgata tcaaatgata ctctattaaa agctcaagaa aatccagaag aatataaaga
     2401 tttattagtg agagttgctg gatatagtac tcaatttgtt aatttatcaa gagaaatgca
     2461 agatgctata atagctagaa attcacatag caatttttaa ttatgaggta gattatgagt
     2521 aaaaaaggta gagttgtaaa agtacaacat ttttcagtaa atgatggaga tggaataagg
     2581 actactatat ttttagaagg atgtaaatta aaatgtaagt ggtgttctaa tccagattcc
     2641 tggagtaaca tagtaaaatt aggtgttatg aaagataaat gtgtatcctg taatagatgt
     2701 atagatgttt gtcctcaaaa tataagttca ctatttgata gagcgcaaat aaataataaa
     2761 tgtgatttat gtggagagtg tatcaaggta tgtcttaaag atgccatttg tataatgacc
     2821 gaagaaatga gtgttgaaga aatagtagag gaagttgaaa aagattttat atttttcttt
     2881 gaatctaatg gagggataac tttttctgga ggtgaaccta cacttcagat tgatttttta
     2941 agagaattag ttgatatttt ttatgataaa ggcataaata ttgcaattga aacttgtgga
     3001 tattttgatt ggaataaggt aaatgatgta tttgagaaaa tagaccacat atttgtagat
     3061 ataaaatcta tggatgataa tattcataaa gaatatacag gagtaagtaa taaaattata
     3121 ttagataata tatgtaggct ttcaaagttg aataaatcta tggttataag agtgccaatt
     3181 atttatggag taaatgatag tgaagaaaat ataagaaata ctgcattatt tgtcaaacaa
     3241 aatgttccag gaggcaagat ggagttactt ccatatcata aatttggaat cgataaatat
     3301 aaggcattag ggcttgaaga ttatatatat gaatttgatg aaatttgtaa caatcatatg
     3361 ttaaaattaa aagagatagt agaacttaca ggagtaaaaa tcatagaata caaatagcta
     3421 tcaggaggat tttaacatgg ataataaatt tcctaatgtg ggagaaaatt taaggttatt
     3481 gagacaagaa atgggcataa gtttagacaa agcttcaaag atgacaggtg tcagtaaagc
     3541 aatgcttgga cagattgaga gaggtgaatc aagtccgaca gtatctactt tatggaaaat
     3601 ttcatctgga tttaaaataa attttactac actattaaat gaaaatacaa atacctatga
     3661 ggttataaag aaagaagaag tagagcctat tgttgagcaa aaagggaaca tgaagttata
     3721 tccaatatac ccttttagcc ctcaaagaag gtttgaactt tttataattg agcttgagga
     3781 aaactgtact catgtttcat ctacacacag tcatgtttta gaagagtatg tactcgtaat
     3841 agaaggtaaa cttgacctta atgtggatgg gaaaacttat attttagagt ctggacattc
     3901 tattaggttt gatggaactt tagagcatat atataaaaat ttaaataaag gaaaaactat
     3961 atttcacaat atagaggttt atagataatt ttatagttat ccttttctaa aataaatgaa
     4021 aattttagaa aaggataact tatttttata atgtcgaaaa gatttatatg tatgtgatga
     4081 tgttattgtt ttaaaaaaaa cataaaagca ttataataat attaacaaaa tagaataaag
     4141 aggaagagaa tatgattata aaggtttcaa tgacagtatt agtattaata ttattgtata
     4201 ttctgtataa gtatgaaact cagcatatag aaacgacaga atatactatt ttcaacaaaa
     4261 aaattccaaa ggaatttgat aactttaaaa tagttcaagt gagtgattta cataataagg
     4321 tgtttgataa aaataataag gtattgatag ataaaattga atcattaaac ccagatgtta
     4381 tatttataac aggagattta gtagatggag aaaataaaaa ttttcaagtt gcattagatt
     4441 taatagataa tttggttgaa aaatatgaag tatatcatat aattggaaat catgaacaaa
     4501 aatctttaat aaaaaagtat aaacatctat ataaggatta ttttaaggaa ctatattcaa
     4561 agaaaataat aaatatggaa aatgaatctg taagaatata tagaggtaat agccatctta
     4621 atttatatgg tttaattata ccattggaat gctatccata tttatttgct aatagcaaaa
     4681 aattagaact aagtgacaat tttgtagaag acaatctagg taaagttaat gaaaatgaat
     4741 ataacattct tttagcacat acaccatttt tctttgaaaa atatgcaaaa tggggagcag
     4801 atttggtttt agcaggacat gttcatggag gtataataag aatacctttt gttggaggtg
     4861 tattatctcc aaatagagag ttctttccta agtatgactg gggaaaatat gaaaaagata
     4921 atagcactat gattttaaat aaaggcttag gaggctcaaa agttttaatt aggcttaatt
     4981 gtaaaccaga gatagttaag attacattga agtatgagaa ataagatttt atataacata
     5041 tataaacatc aataacatat aaataaggtt gatgttaaat ccatttaaac atatataatg
     5101 accaattttg aataattgtt gaattaataa aaataaagtg ctatttttag catagaatat
     5161 ttaaactatg gggggtagta aagaatcatg ggtgatacaa gtagatttta tgagtctgta
     5221 aaaaataaat atccgaaatt aaaagatgga actagggttc atctgtggcc aaaagaaaac
     5281 attataattg gtcctattta tgatgaagaa gatggatata aaaaaagtat cggaaatgtg
     5341 gatagcttaa gttggaagca aattgttata ctggctgaat gtgatgggaa aaatactact
     5401 gacgatataa taaatatatt atccaataga tatagaaata tccataatat gaaagaaaaa
     5461 gttatggaat tttttatgtt ttatgagaat gtatacttaa cgtttgaaga tgatatacag
     5521 acactatcaa gtgtatttga gataacagga aataaatcat acattactcc tttgtacttt
     5581 actatagaaa tagatggaga taataataca aatgattatt tttctgaaat cacaagttta
     5641 ttgaactgca tgtatgagaa aggctgtaga tttatagagg ttattggtga ggatatttta
     5701 aaaaataaaa gtatgagaga aatatttcaa tatatgttag accattttga tttaattgtg
     5761 gtaactaaag atagttttac tattgataga agcttaataa aggaattaga taattatcgt
     5821 cataaagtga tatggaaagt atattcaaaa aatgatgata cacgtattaa attaaaagaa
     5881 gatataaaaa taaccagttt aataaaaagg ggtcacactg tagtaagagg agacagagaa
     5941 aaagcaataa aaatagaaga tatgaggttt aaagatacaa aggagaggtt ttcaaaatat
     6001 ggagcagagt ggagtcattt atatatttct tctgatggta gtgtaaaatc ttattcattt
     6061 caaaaagata aaagaatcta tttaggcaat atatttaaca attctgttga agaaattttc
     6121 ttagaaatgc aaaataaaat tgacaaacta gaaattgtgt atcaataggg ggaaagttta
     6181 agatttatag aagatttata gattgtttat ttaagtttgt gtcaataatt ataatgaggt
     6241 gagattactt gttaaaatta ttggacaaat ttttttatga tgttctactt aaattttggc
     6301 tgtttatgat gctgtggatt tttagagaac tttttacaaa tttcaaaatg actaaaaaat
     6361 ttaaatttca taaaatattt atgtaaaaat catataaaat tcatatagca tatgtatcat
     6421 atgaggtgta gaaagactag cttaaaaacc ataatataat accctattgt aatgataacc
     6481 ctccctcaca tcctcaaata gagttatcat tacaagaatt aatccataaa tttaaaattt
     6541 ttatgaatat attttattat tattgttatt ttattcattc taaaataggt aaaactcaat
     6601 atataaaaat acttttgtca tttaaataaa gcctaaatag tttttaagat tattttagac
     6661 acgttttaag tcataacagt atcagctatt ctaaatatcg tcaaagtagc cgcagttcct
     6721 gctgattgcc catttcgtat ccctactgtt gtagatgaaa aattatttat tgtaaaagta
     6781 gtcccaccaa ataaaaaacc agcaaaatat ccagaaagta ctcctccgcc tcctggctca
     6841 gtagtaagcc ctgccaaatc ttttcctcct aaaattggga ataggatagc gaaagaatat
     6901 tgatttatac ttgatgaacc tggttgtaat gctactgaaa ctgatatata atacacacca
     6961 ttttcttgta ctgtaaacgt accatttgca tctactgtta tacttgaact atttcctatt
     7021 attgtttgcc aacctgttat agcatctcca ttatttagtg tttgtggaga tggtgcattt
     7081 acgaggaagt tagcaactgg ctgagaggaa ttgcctgttg ctcctgttgg acctgttggg
     7141 cctgttactc ctgtcgctcc tgttgctccc gttggtccta tacttcctgt cgctcctgtt
     7201 ggacctgtta ctcccgttgg ccctatactt cctgtcgctc ctgtatttcc tgttggccct
     7261 atttctcctg tcactcctgt tgggcctgtt gctcccgttg gtcctatttc tcctgtcact
     7321 cctgtatttc ctgtcggacc cgttgctccc gttggtccta tttctcctgt tactcctgta
     7381 tttcctgttg gacctgttgc tcccgttggt cctatttctc ctgtcgctcc tgttgggcct
     7441 gttgctcccg ttggtcctat acttcctgtc actcctgttg ggcctgttgc tcctgttggt
     7501 cctatttctc ctgttggtcc tatttctcct gtcgctcccg ttggtcctat acttcctgtc
     7561 actcctgttg ggcctgttgc tcccgttggt cctatttctc ctgttggccc tatttctcct
     7621 gtcactcctg tcgctcctgt tggacctgtt gctcctgttg gtcctatact tcctgtcact
     7681 cctgtcgctc ctgttgctcc cgttggtcct atttctcctg ttggtcctgt tgctcctgtt
     7741 ggtcctatac ttcctgtcac tcctgttgga cctgttgctc ccgttggtcc tatttctcct
     7801 gtatttcctg ttgggcctgt tactcctgtt ggtcctatac ttcctgtcac tcccgtattt
     7861 cctgtcgggc ctgttgctcc cgttggtcct atacttcctg tcactcccgt atttcctgtc
     7921 ggacctgtta ctcctgttgg tcctatactt cctgtcgctc ctgtacttcc ggttgggcct
     7981 gttactcccg ttggccctat acttcctgtt gctcctgtat ttccggttgg gcctgttggt
     8041 cctatgcttc ctgtcgctcc tgtacttccg gttgggcctg ttactcccgt tggtcctgta
     8101 cttcctgtcg ctcctcttgg tcccattgga cctgtaattc taattggaag acactcacat
     8161 ttaggataac aattatctat aattctatct tcaaatgtta caagtatata cggtttgtta
     8221 caacctctac tagatgtaaa ttcaagaatc tgataagggt aatttaaaga taaagctaac
     8281 ccataattag gatatttatt cattgcccat gcttttatag ttcccgtaat atttaaacaa
     8341 atatagttgg atgtatcact tatcccaact ggtaaatatc ccctaatatt ttccatagac
     8401 actctatcat tccatgttat agttcgtaaa ttataatatt cacttattct tttaatttca
     8461 atatctactt tttctttaaa aatatgattc ttatttgaat caatatgaat aaataactct
     8521 gcttttaata tacttttgtc ttttaatata tctggtattc tcactttaaa aaaagtaatc
     8581 aacttttctt ttacaaaact atttgaacat ttacttccta ttaagcaata atctaaatta
     8641 ctaaagtttt tatctggata ttttttagat ataaaagtat catcatttaa ataaagtata
     8701 atatttctca tttataatcc ctccattttg ggtctatatc atactatgtt taaaatacat
     8761 gcttagttac aatataatta aaatttatag tattaaattt ttatataata atgctgattt
     8821 ctacaaaaaa agaacatgtc attagaaatt tctaatagca tgttcttttt cattattata
     8881 tattacactt taaattattc cttgactggc ttagctggtg taaactaaac cagtctttct
     8941 aaaatattaa gcactttttt cttattatgt tttttaggat ttattgtcta tattattgta
     9001 aaccaaagtt ataaaattgt aaatagaata tattaccata acaatatctt tttataagta
     9061 aaaattataa aaaaccttat ttttattaaa tagaataaat aaaataagtt taaaaaatac
     9121 tgaaataagt gt
//
LOCUS       DAYAOX010000143         9125 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.143, whole genome shotgun sequence.
ACCESSION   DAYAOX010000143 DAYAOX010000000
VERSION     DAYAOX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.143"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..448)
                     /locus_tag="ACGI57_002643"
     CDS             complement(<1..448)
                     /locus_tag="ACGI57_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFG9717713.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALV"
     gene            complement(575..1264)
                     /locus_tag="ACGI57_002644"
     CDS             complement(575..1264)
                     /locus_tag="ACGI57_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9717714.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(1272..1835)
                     /locus_tag="ACGI57_002645"
     CDS             complement(1272..1835)
                     /locus_tag="ACGI57_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFG9717715.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(1837..2805)
                     /locus_tag="ACGI57_002646"
     CDS             complement(1837..2805)
                     /locus_tag="ACGI57_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFG9717716.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(2906..2949)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3315..3944
                     /locus_tag="ACGI57_002647"
     CDS             3315..3944
                     /locus_tag="ACGI57_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9717717.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            3919..4323
                     /locus_tag="ACGI57_002648"
     CDS             3919..4323
                     /locus_tag="ACGI57_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HFG9717718.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            4326..4802
                     /locus_tag="ACGI57_002649"
     CDS             4326..4802
                     /locus_tag="ACGI57_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFG9717719.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            5374..6039
                     /locus_tag="ACGI57_002650"
     CDS             5374..6039
                     /locus_tag="ACGI57_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFG9717720.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLRQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            6061..6993
                     /locus_tag="ACGI57_002651"
     CDS             6061..6993
                     /locus_tag="ACGI57_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFG9717721.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            7014..7655
                     /gene="pcp"
                     /locus_tag="ACGI57_002652"
     CDS             7014..7655
                     /gene="pcp"
                     /locus_tag="ACGI57_002652"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFG9717722.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(7775..8107)
                     /locus_tag="ACGI57_002653"
     CDS             complement(7775..8107)
                     /locus_tag="ACGI57_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9717723.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            8373..8993
                     /locus_tag="ACGI57_002654"
     CDS             8373..8993
                     /locus_tag="ACGI57_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9717724.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
BASE COUNT         3355 a         1332 c         1200 g         3238 t
ORIGIN      
        1 taactaatgc aaatctaatt tcccatggat tctttgaata aacatatttt ttaagtatat
       61 catacatatt ttctttatat tttttcatag actttaaatc agaacaaaat ccatcgcaaa
      121 ctgaccaatt atcgatttta ggtataaatc tttttacata gtataaagtt tcttcaaaat
      181 tactatttat attgccaatt accatacctt gtagcctaac ttcttcatag tatgtatcct
      241 gtgctacttc taaaaattcc ttccaattcc cttggcttat ttcttttgat aactttctta
      301 aattaggaac tctaatacct attatatttt ctgttccagg tataagcttt ttattaaaat
      361 ctctatattt tatatcttgt atagacatca tatattctaa aagttcatta taatccaatt
      421 tattccattt tcccctatca aacatcattt aattgctcct taattccttt tttcttattg
      481 taacataaaa ctctacaaaa aaagcttcta actattagaa gctttttcaa aaacataaga
      541 aacttttcaa ttttaaattt aaactaaatt aatgctaatc tcctacacca aagtctgtat
      601 tattgtataa aacttctata tttggatttt catccaagaa tttatactta ctaacaaacc
      661 atttcactaa ctcttcatct ctttttatac tagaagaatt atttataaaa acattcactg
      721 tcgctttttc aaaagtattt aggataatct ctatatcttt atctatcatc tcttttgttt
      781 gtccttttat tccaaccata atacaaggtg aatcaaaata ttcttttaca tcttgtggag
      841 tcttaaaatt tgcattttta tttaaaatat tatttctaaa atcattgtca aaggtttcaa
      901 ctccaatttt aaataccaca ggaatttcaa aatactctct catttcctta agcctatttt
      961 tgtaagacca atgactttct aaaaataatt tttttatatt cttctctttt ataatacact
     1021 ttattttttc taaagtatcc ttaggtaatt caaaacagct tccagaattt atgacttcaa
     1081 gaactccata tttcccagtt acgtttttta aaacctttag atttaattta ttcatctctt
     1141 cttctaaatt agaattatca tctatataat cacaaaaaga acactttccc catatacaag
     1201 gaaatccttt aagcagaaca atttctcttt gatttttatt tgttatctca ctatatctat
     1261 ccattttcta tctattttcc tccagtcaat gataccttta aagtttttgg aactgcattt
     1321 aatcctaaat tcactaaaac aactgctaac agcttatctc cataatcagt aaatacttgg
     1381 gttacaaaca cactaaaaac atcccccagt cctaacactt tcaagatttg cactatatat
     1441 gatgaacctg aagaggtcat acctccaaat aaaaatgctg aaattattga acttattata
     1501 gatgttggaa tggcaaataa aagtactcct agtggagttt ttaaacccct taaaaaattc
     1561 tttttaaatg ctattccagc aagtaatccg gttgatattt gaactggtgc aaaatagaga
     1621 gaatacacat caaacgtaat tccacttatt aaacttccaa acacacctgt aacaacagcg
     1681 tatttaggtc ctaaaagtga agctatcatt atagttccta ttgaatctaa gtatacaggt
     1741 aatcttaatc ccatagctat aaaagcgcct attatattta tagaaatacc aaaagctata
     1801 agtgtaatag tttttacaca tatttttctt ttcacactat atcactcctt ccacagagtc
     1861 tataatgtct tcataacctt taaaaagcct ctttaaaaac atgtacataa atttcttctc
     1921 atcaacttca tttagaacaa ttgcattagg atttttttta tagaaattga aagaatccac
     1981 tatagactga cccatagcta ttccatcttc tacaacttct acatatgatt caaatccttt
     2041 acatatactt ctatctataa agtatgctac tgctagaggg tcatttatca cacagccaat
     2101 tattccctcc tgttcccaat ggaaatcaat ataaaatcta gttatttcag ttatatactt
     2161 tgccatcttc ttatcaagtc tatttataaa ctcaataata ttaggagtaa gtacaatttt
     2221 tctagttaca tctaaaccta ccatgtggat ttttttagat aaattcttgt aaacataatc
     2281 tgctccatgt gggtctaccc aataattaaa ctctgctact ggagagcaat ttccatgaat
     2341 cctaaatgct cctcccatag atacaaactc atcgagattt tcaaatgctt tcttatcttt
     2401 aataagtgct ttagctatat ttgtaagtgg tgcaagtgct attattgata ctttttcatg
     2461 attatataaa gtatttatta taaaatccac tgctccattt tttgctttag ctcctacaac
     2521 cttttgataa aagttttctc caataccatc ttctccatgt gtatcttgag ctgttacaag
     2581 ttttcttttt agtggtgctt cttctcctat atatactgga atatttagtg aagaacacat
     2641 ttgaagtgtt tttagtgcat tttctgcacc tatatttgct gggacatttc cacaacatgt
     2701 ggtaattcca attacttcta gctctggtga gtttaaagcc agaagaattg ccaaagaatc
     2761 atcaattcct gggtcacaat caattattac ttttctcttt tccatttttt atcctccttg
     2821 atttgtggtc gaggagaact acacatcttt tttccacttt caaaaaggtg tgtctaaggg
     2881 tattaattat atactgccac tatataattc cttagttttt tatacttgga gttctcgaac
     2941 aagtacagat gtaaaatcat atttttaggc tacttactat aagactctta aaaactatag
     3001 accgttaaaa aatttttaaa catctgattt aatagctata aaataaattt tatagcataa
     3061 aagaatttat tatattttaa tgtatcttat taaagtttat ttagaattat gaaccaactt
     3121 taataagatg tggcgcattt aaaccgatta ttattataca ataatatctt aaatattaca
     3181 attaataaca ttaatttatt atccataaaa atatattaac ttatattatt taataattta
     3241 ttgacagtat taaaattaaa ttatacaata acagtgtaaa ttaacttaac accgttaagc
     3301 aaagaggtga ttatatgagt atcaaagaaa gacgtaaaaa tgaaaaagaa gaaatgaaga
     3361 aaaagattat ggatgctagc atcgaaatta taaaccaaca tggatatgaa aatttatcaa
     3421 taagaaaaat tgctacaaaa atagaatatt ctccaactac aatttatctt tactacaaag
     3481 acaaagcaga gattatcagc gatatgacca ataaacttta taacacagta gaaagtaatg
     3541 ccattgacat tatgaataat tgctcatcac taccaataga taaacaaatc atagaaatta
     3601 tgactagctt tataaaaact ctttctagtg aaccagagat ggcaaaatct atcatgcata
     3661 gtagaatgaa tattatattt gcatctgaaa atacaaacaa tacgcctagc aataatggta
     3721 taaaaatgct tgataaattc ctttccattg gaattgaaca aggaatattc aaaaaaaata
     3781 tagataaaag ctcatggatg cttataagtg cattgttagg attcgtttta tgtgttgttg
     3841 aaaataaact atattcttta cataattttt ctcaacttat agaaaatttc ttagatattt
     3901 tagtgggagg actttacaat gaaaattcgc aacaaggcta ctaataaaaa gaaaaaatat
     3961 gtaaaaatat ctattgctct attctttgta tttatatgca ttattattgt aattgtaagc
     4021 aacttaaagc cagaaaactt ggtagtaaaa gatatagata ttaataatgt taaaaatggc
     4081 atttatactg gaagtgctga taataacttg gtaaaagcta ctgtgtctgt tgaagttaat
     4141 aatggtaaaa tccaaaatat taatatctta aaacacgacc atcttttagg taaacctgct
     4201 gaaaaaatta caacaagtat tattaaacaa cagtctttag atgtggatgc tataacttct
     4261 gcaacttata gcagtaatac tatacgaaaa gctgtagaaa atgctttaag aaagggagaa
     4321 taattatgaa tactattatt atatattcaa gtaaatatgg ttgtacaaaa gactgtgcaa
     4381 atatactaaa aaataaacta tcagataatg tcactattgt tgatattaat aataataata
     4441 ataaaattga gttatcaaaa tttgataaaa ttattattgg aagttctata tatgttggtt
     4501 cagtttcaaa aaaaatacag gtgctatgta atgataatgt agagttatta aacaaaaaac
     4561 aagttggcat tttcctttgc tgtggctttt ctgaacaagc agataaatat ttaaaatcaa
     4621 attttccatc atccttatta gaaagtgcaa atgctattgg aatttttggt agtgaagctc
     4681 gattagaaaa aatgaaattt ttagataaac ttattatgaa ggctgtttca aaaggtaatt
     4741 atgacagttt tagaatatca caggataata tagataattt tttaataaat cttaatagct
     4801 aaaataaagc taattatttc tatgataaat tcttttattt ataagtaata ttgctcaaaa
     4861 tagtctttaa actttatcaa aattttattt ttcttagtga aatctaatga aactagcact
     4921 taatatatgc tttattaatc ataacaaata tgttttatta atcataacaa aaagagagtg
     4981 tctaatgaac taaaaaattc attagacact ctctttttgt atgaaatcaa atatattttt
     5041 atcatttcaa cagagaaaaa tacacttgtc tctatgtttg gcacattctc tttttataat
     5101 ataatgttta atttaaaact actatttcaa atattgacat atacttaaac tatagactta
     5161 caaaatcaat tcatattagc ttaaaatata atctttgtta ttcaaattcc tatacacaac
     5221 cattatttac tttgatactt taaaacatct tccctatttt ttattaaatt ttataataat
     5281 agtcctatta acgcaaatac tataaaaaga gtttaacttt atccattaaa taaatttaaa
     5341 ttaaaacata tagaaatgga aggagaacaa tttatgataa aactaatcgg tatattgata
     5401 gttgtaattg gatttatact taaaattgac acactattta ctgtattatt agcaggagtg
     5461 gcaacaggaa ttgttgcagg attagatttt aatcaaattt ttacaatttt aggagattct
     5521 tttgtctcaa acagaggtgt atcattattc atattaacat tgccagtaat cggtgtatta
     5581 gaaagatatg gtttaagaca aagagctgtt tctttaatag aaaaattaaa aagacttaca
     5641 acaggaaaag tacttactat atacatgata gctagacaaa tagctggtgc tctatcaata
     5701 agaatgagtg gacatcctca atttgtaaga ccacttgtta atcctatggc acaagctgct
     5761 ggtttaagta atagtgatga actaaaagaa tcggatgaag aagccataaa ggcattatct
     5821 gctgcatcag aaaactatgg aaatttttat ggtcaaaact tatttgcagg tagtagtgga
     5881 gtattactta tagcatctac tctgactcaa tttggataca atgtaactgg acttaatatc
     5941 gtaaaggcta gtactattat ggcagtaata gcacttgtag tggctacaat ccagtttacc
     6001 ttatatgata aaaaattaaa caaaagtcat aaaaaatagt tttatttagg aggaataaaa
     6061 atgcaaaatt ttataaacat atcattggaa atcttttacg cactaatggg atttttaatg
     6121 attgttatag catataagtc ttttactacc ataaataaca ataagaaata tggtacatca
     6181 ttattttgga tacttatatc actgccattc atatttggta gattgattcc agccaatata
     6241 ataggaataa tattaatatt atctagttta ttaacacttt ctaaacaagt tgtatttgca
     6301 aagtatgaag aaccagatga aaattttgga aaagaacaag cagacaaact taaaaataaa
     6361 atatttatac catcattaat attagcattt gctgcagtag tagtagctat gagtctttct
     6421 aattttgata attcttctca gtttgcaata ggtgtgggtt caataattgc attaatatca
     6481 gcccttataa taactaaggc aaaacctgct acatcagttc aagatggctc aagattactt
     6541 caacaaatgg gacctgctag tatgttacca caacttttag ttgcattggg agctttattt
     6601 actcaagccg gagtcggtga agttatttca accatgatat ctggtgtagt ccctgctgat
     6661 agcagattat ttggtgttat agcttatgta ttaggtatgg ttatatttac catgataatg
     6721 ggaaatgcat ttgccgcatt ttcagtcata acggcaggaa ttggtattcc ttttgtattg
     6781 tctcaaggtg gaaatcctgc tataattgga gcattagcat taacagcagg atattgtgga
     6841 actctactta ctccaatggc tgctaatttt aatatagttc cagcagcctt gctagaatgt
     6901 aaaaatgatt atattgttat aaaatatcaa gctccagttg ctttggtatt aattatagcc
     6961 cacatattag taatgtactt tttaggattt taatataatt aggaggtaaa ataatgaaaa
     7021 ttttgttaac aggatttgac ccatttggcg gagaaccaat aaacccagct caagaagcag
     7081 ttgaaagggt caataataat ataaacggtg cagaaatcat aaagataact ataccaacag
     7141 ttatgacaaa atctgttgaa gctatagata aagctattca agagcacaat ccagatatag
     7201 taatatcagt tggacaagct ggaggtagat ttgacattac acctgagaga gttgctataa
     7261 atatagatga ttttagaata aaagataatg agggtaacca ggttatagat actattataa
     7321 aagaagatgg tgaacctgca tatttttcaa aattgcctgt aaaagctatg gtaaaacata
     7381 tgaatgaaaa taaaattcca gctagtgttt caaacactgc aggaacattt gtttgcaatc
     7441 atgtgatgta tggaattctt tatatgatag acaagaaata tcctaatata aggggtggat
     7501 ttatacacat cccatataca acttcacaag taatagataa aaaaaataca ccattcatgt
     7561 ctttagaaga aatagtaaaa ggcttagaat tagctataga agcttgtatt atatataaag
     7621 aagatgtaaa agaaattggt ggtgaaattt cataataaat tttttagtat ttttataaat
     7681 taaaaatact aactattaat ttaaatatag actgtgagta gattattcaa acaattttac
     7741 tcacagtctt tttatggtat ctacagatac aaaattaagg tctattcctc tctccccact
     7801 cacacattgc atctaaaatt ggaatcagag attttcccct ttctgataga ctatattcta
     7861 ccttaggagg tatttgagga tattcagtac gaataatcaa gttatctgct tctaattctt
     7921 ttaatgataa actcaaagtt ttataggaga tagtatttat ataacgtttt aatgcattat
     7981 atcttacaac ttcaaattct acaagacagt aaagaattat catcttatat ttaccactaa
     8041 tcagtgaaag tgtataacta aatcctgttt cacttaaatc tgtatttgat atacagtcta
     8101 tactcatata ttacactctc cttttggtta gtattgaaca aaaatgtgcg tacttgtttt
     8161 tagaataatt tataataaaa ttatagtgta agaataattg aaagtcaatt ttataatagg
     8221 aggtgtattt actaaaaaaa aagttatttt aaataggatt ctaagattag acatttctat
     8281 ataaattgat aatattcatg aaaatcctat tttataaaaa ataatgtctc ttagaaaatc
     8341 tattaaataa aattcattgg aggaaattta taatgaaagt tttattgata aacggtagcc
     8401 caaaccaata tggatgtact tatacagcac taaatgagat tactaaagta ttgagtaaac
     8461 acgatattaa aacagaaata ctgtatttag gtaaagaaac aatcccagga tgtattagtt
     8521 gcgcatcctg ttttgaaact ggaaaatgta ttcgaaatga taaagttaat gaattgattg
     8581 aagatctgga taatattgat ggtattatta ttggttctcc tgtttacttt tctagtgcaa
     8641 ctggtcaatt aacttctttt cttgaccgtc tatttttcat agctgggagt cgcatggcta
     8701 caaaattagg tgcttcagtt gtgtcatgta gacgtggtgg tgcaagtgca acatttgacc
     8761 aactaaataa atatttttca atcagcaata tgcctattgt atcatcacag tattggaacc
     8821 aggtacatgg ctttactcca gaggatgtta tgaaggatga agaaggatta cagactatgc
     8881 gaactctagg agaaaacatg gcatggcttc ttaagtgtat atcagcaggt aaaaaagctg
     8941 gaattaaaga accacaatat gaagaaacaa ttatgactaa ttttataaaa taaataaaaa
     9001 atgtactaat attaattaaa acaaaacact aatttataaa taaaataagc ataaatgtag
     9061 gataaaaagc actaaaacag aggtattgaa ttttatatca aacctctgtt ttattattac
     9121 attaa
//
LOCUS       DAYAOX010000144         9080 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.144, whole genome shotgun sequence.
ACCESSION   DAYAOX010000144 DAYAOX010000000
VERSION     DAYAOX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.144"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            126..578
                     /gene="accB"
                     /locus_tag="ACGI57_002655"
     CDS             126..578
                     /gene="accB"
                     /locus_tag="ACGI57_002655"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFG9717725.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            594..1955
                     /gene="accC"
                     /locus_tag="ACGI57_002656"
     CDS             594..1955
                     /gene="accC"
                     /locus_tag="ACGI57_002656"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFG9717726.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            1970..2824
                     /gene="accD"
                     /locus_tag="ACGI57_002657"
     CDS             1970..2824
                     /gene="accD"
                     /locus_tag="ACGI57_002657"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFG9717727.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            2845..3789
                     /locus_tag="ACGI57_002658"
     CDS             2845..3789
                     /locus_tag="ACGI57_002658"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFG9717728.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            3978..4238
                     /locus_tag="ACGI57_002659"
     CDS             3978..4238
                     /locus_tag="ACGI57_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HFG9717729.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            4391..4762
                     /locus_tag="ACGI57_002660"
     CDS             4391..4762
                     /locus_tag="ACGI57_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFG9717730.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            4792..5637
                     /locus_tag="ACGI57_002661"
     CDS             4792..5637
                     /locus_tag="ACGI57_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717731.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            5948..7075
                     /locus_tag="ACGI57_002662"
     CDS             5948..7075
                     /locus_tag="ACGI57_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9717732.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            7179..7814
                     /gene="lexA"
                     /locus_tag="ACGI57_002663"
     CDS             7179..7814
                     /gene="lexA"
                     /locus_tag="ACGI57_002663"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFG9717733.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(7961..9079)
                     /locus_tag="ACGI57_002664"
     CDS             complement(7961..9079)
                     /locus_tag="ACGI57_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFG9717734.1"
                     /translation="MKTLILLLIVSISIYLTGCTTSDNGNNQSNNNDSLSSSISSDKN
                     VKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGVKDIKYLIATHSHA
                     DHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPSVPLENNKFYLEDS
                     YFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILPKLEKVDLLKVAHH
                     GSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGVKVHRSDECSDIIF
                     ESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNTSNSEEIVYFTPKG
                     KSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
BASE COUNT         3467 a         1065 c         1499 g         3049 t
ORIGIN      
        1 ggttgttaat ttaaaattaa aagatatatt gatgtgttga ttctctagtc agcacttttt
       61 cttttataaa tttgtttttt gtgatataat attagcacca ggtaataata caagggggta
      121 acaaaatgaa catcaatgaa attaaggaat tattaaaggc catagactca actaacttag
      181 aatatgtaaa acttgaaagt agcgatttaa gattagaagt atctaaaaaa gctcaatcta
      241 caagttcacc tgttttaagt gtacaacaag aatcagtagt ggatctttct ttagaaaagc
      301 cagttgtaaa cgatacacct gtcacatcta atgagaatct atcagtagta gtagctcctt
      361 tgatgggaac attctatgat tctccaagtc cagatgcaga tagctttgtt aaagttggcg
      421 atgtagttga agaaggcgac actttatgta tattagaagc aatgaagctt atgaatgaga
      481 taacaagtga aattaaaggt gaaataattg aagtattagt tagtaatgaa gaactagtag
      541 aatataatca acctttattt aagataaaac cattgtagta ggaggtattt aagttgataa
      601 aaaaaatact agttgcaaat agaggcgata tagcagttag aatcatcaga acatgtaaag
      661 aactaggtat aaaaactgtt gctatctatt ctgaaataga taaagattgt tttcatagat
      721 atatagctga tgaatctatt tgtataggac caaataatat aagtaagagt tataataata
      781 tagaaaatat aatctatctt gctcttaaat taaagtgtga tgctattcat ccaggctttg
      841 gatttttatc agagaatcct gaatttgcaa agcaatgtga agataataat attattttta
      901 taggtccaac aagagaacaa atgattttga tgggagataa atctagagca agagaaacta
      961 tgatggaatt gaatatccct gttgttcctg gttcagaatc tgttttgaaa actaaggaag
     1021 aagctttaga agttgcaaga gaaattggat atcctgttat gataaaagcc tcaagtggag
     1081 gcggtggaaa aggtatgaga atcgttagaa aagaagaaga acttttttca aattttgata
     1141 tggcaagttc agaagctcta gcagcatttt ctaatagtga tttatatatg gaaaagttta
     1201 tagaaaatcc tagacatata gaagtacaag tatttggaga taaacattca aatgcaatac
     1261 atttaggtga tagagattgt tctatgcaaa ggcgaaatca aaaagtaata gaagaatctt
     1321 taagtccata cctttcagat gaagaaagac aaaaacttca taaaattgca gtagatatag
     1381 taaaaggagt aggttatatc ggtgcaggta ctattgagtt tatagttgac aaagataaaa
     1441 acttttattt tatagagatg aatacaagaa tacaagttga acatccagtt acagagatgg
     1501 taacaaatct tgaccttata aaacttcaaa tatctatagc taatggggat aaaattcctt
     1561 ttaaacaaga agatattact tttagaggtc atgctattga atgtagaata aatgcagaag
     1621 attcatcaaa gaattttgct ccatcaccag gtaagataga atcattaaat ttacctggag
     1681 gttttggtgt tagatttgat acttttgtat atgcaggata tactatacca cctttatatg
     1741 attctatgat aggtaagtta atatgttggg ctgaaactag agaagaatgt ataagtagaa
     1801 tatatagagc tctagacgaa attattgtag aaggtattaa tacaaatgta gaatttcaaa
     1861 aggcacttgt aacaagtgag gagtttagag aagatactca tcataccaaa tttatagaag
     1921 atgtctttat gaagaaagaa tttgctacat tataaggagt aaatagatta tgataaaaaa
     1981 atttttatca tcaaaagaat ctaagtatgt tacgatttct ttagatgaca actttaaaaa
     2041 gaatagtgta gatgataaat tttggaccta ttgcaaaggt tgtgatagtc atgtatttag
     2101 aaaggacata gaagaaaatt catttgtatg tccaaaatgt tcaagacatt atggcttaag
     2161 agcaagaaaa agaataaatt tattgattga taaaggaact tttatggagt ttaattctga
     2221 tgtagaattt caaaatccac taaattttcc taaatacaaa gaaaaagtag actcttataa
     2281 ggaaaaaaca aaagagtcag aagctgttgt cacaggttat ggaagaataa atggaattaa
     2341 aacagttatt tgtgtaatga atcctgattt tatgatggga agtatgggtt ctattgttgg
     2401 ggaaaaaata acttattcaa tagagtatgc tgctgaaaat aatttaccta taattatttg
     2461 ttctgcttca ggaggagcta gaatgcaaga aggtatggta tctcttatgc agatggcaaa
     2521 gacttctcaa gcactttcta agcttgaaga aaaatcattg ccatatatat cagttctaac
     2581 tgaccctact actggaggag taactgctag ttttgctatg cttggtgata ttataatttc
     2641 tgagccaaat acgttgatag gttttgcagg tccaagagta atagagcaaa caataaatca
     2701 aaaacttcct gaaggatttc aaacttctga gtttttactt gaaaaaggtt ttattgacat
     2761 gatagtagat agaagaaaaa tgaaggaagt attatatcaa attcttgcta tgcacaagaa
     2821 atagggcata aaggaggaac tgttttgatt gaaaataata gtgaaaaaat aaaagtctta
     2881 gaaaatgata ttaaacaatt aataactatt tctaaggcaa ataatataga tttaagtgat
     2941 aaaataaatt cacttaatga gaaattggaa aagcttaagg aagatgcttt ttcacatcta
     3001 tcagcatatg aaaaggtaac attaagtaga gatataaaga gacctacgac attggaatac
     3061 atagaacata tttgttctaa ttttttagaa cttcatggag ataggctttt taaagatgac
     3121 ccttctatgg ttggaggaat tggtcagata ggaaagttta atgttactat agttggacac
     3181 caaaaaggaa gagatacaaa agaaaatata aaaagaaatt ttggaatgcc tcatccagaa
     3241 ggatacagaa aagcattaag acttatgaaa caagctgaaa aatttaatag acctataata
     3301 acgtttattg atacatcagg agctttttgt ggtctagaag cagaagaaag aggacaagga
     3361 gaagctattg caagaaactt acttgaaatg agtaaacttt ctgttccagt aatcactttt
     3421 gtaattggtg aaggtggtag tggaggagct ttaggtatcg gagtaggaaa tgatgtatgt
     3481 atgttagaac attcagttta ttctgtgatt tctccagaag ggctttctag tattctattt
     3541 aaagattcat ctaaagctaa agaagcttgt gatgttatga agcttacaag taatgattta
     3601 tatgatttaa aaataataga taagattata aaagaacctc ttggaggagc acaaaaagat
     3661 gtagaagctg tttcaaaaga aataaaagct tacattttag aaagattaaa tcattacaaa
     3721 gatatggata aagaggaaat tatagctcaa agatataaaa aatttagaaa tattggaaaa
     3781 tgcttataga atataaagaa atttgtagtt ttttcataat aaaatggtta aaaaaaataa
     3841 aggttttctt aaaacgatta tagaagtata tttttataaa agtatacttt aaatgatgat
     3901 tgtaattagt aaatggcaat tatgatttag tactttaggt aatggatact ttaacaaatt
     3961 aacaaggaga tgttgaaatg gaggtactaa aagtttcatc aaaatctaat cctaattctg
     4021 tagctggagc cttggctgga gtactaagag aacgaggcgt tgcagaaata caagctatag
     4081 gagctggagc tctaaatcaa gcgataaaat caattgcaat agcaagaggt tttgttgcac
     4141 ctagtggaat ggatttagtt tgcattcccg catttactga tatagaaatc gaaggtgata
     4201 agaaaacagc cattaaactt ataattgaac ctagataata tatctcttta ttagaattta
     4261 attaagtttt aaaggttggc tatagaaata ggacactata gtgaaccttt tttactttat
     4321 atgttttatt ttatgtaact tttaatgtat aatcttaagt aaacaaataa gattggagga
     4381 aatatacatt atgaaattaa aattattggt agaagagtat gcagtatgta gattaaataa
     4441 tgacagtaaa ataccaacat ggatagatac agagaagttt tactctataa ctagaactga
     4501 tgatgaatta agtgttgtat gtttaaataa taatattcct tctgatgtta agtcagaaaa
     4561 ggagtggaga atacttaaga tactaggtcc tttagacttt tcacttgtag gaattttatc
     4621 taagttaagt agtttgttag cagacaacaa aataagcata tttgcaattt ctacttatga
     4681 tacagactat atcttagtaa aagaaaagga tatagaaaat gcttgtaaaa ttttaagctg
     4741 taatggatat gaagtagagt agttaaatac ttttacatga ggatttaact tatgatttat
     4801 ataatcgaaa ttttaaaaat aagtgtaata aatatttttt gtttcatagg aatatttatt
     4861 gtatttggat tgatttttag cattacagaa aatctaaata ataaatttat attttcatct
     4921 tttggaaaaa ctggaattat tgtaactgga gctataggaa catttgttca tgaattgagt
     4981 catttgataa tgtgtttaat tttcatgcat aaaataaatt cagttaagtt tttcagacca
     5041 atagaaagta aaaatgatgg tatactagga tatgtaagtc atagctataa aaaagataat
     5101 ctttatcaaa gtataggaaa tttctttata ggaattgcac ctattatagg tggaacaata
     5161 atgattattt taatttttaa gatattactg ccagatagtt atatgaaagt gactcaaaat
     5221 atagatttaa agttatatgt aagcatgatt aataatttaa atataaatgg ttttatacac
     5281 attattttag gtgatatttc caattttata agaataatgt tgttaacacc tagtgtatat
     5341 tcattgaagt attttatatt tatgttttta atgtattcta tatctacaca tatgtcactt
     5401 agtagtgctg atttaaaaaa tagcttaaat ggcctgagct ttatagttat agttattttt
     5461 gtaataagtt caattacata cttatttgga tttgataact tgaatatatc cagtttaata
     5521 gttaagtata acatatttat aagtttcttt atggcaatag ggtttatttt ttcaatagta
     5581 actctcataa tatcctatat tttaagctta gtcagcccat ttaaaaactt tagttaaata
     5641 aatacatatt ttactaatat ttggtttcgt tagaaacata taaaaatatt atataaaaaa
     5701 gattctagta gagcataaaa aatttctcaa tatatttata taaatatatt gagaaataaa
     5761 aaagagtact taaattcaac tagtctttaa atgtggattt agggaatagt tagtgataat
     5821 ttcatctaaa gcactgctaa tctcttaaaa atgtaaaaaa tgattaaatt aaatatttat
     5881 actcacttag tataaatatc aaaaaataat ataaaagatt tatattttag ttttaggagg
     5941 tttaattttg aataatgata agaaaattat aaaagtacat aataaatcag ataaacctga
     6001 taatattgtt atcaaagaaa atgaactcat agaaaaatgt ctacttttgg aaaataaact
     6061 tcctgtattt atgaaggatt attttatata tttaaaaggt tctgttgcag tttctacacg
     6121 acttgcttat ttagaagata ttaatttttt ttgttcatat ttgattgaaa ccaaagaatt
     6181 aacaaatgca gattgtgtaa aagacattac agaagatgat tttaatacaa taaaatcaag
     6241 agatataaat ttattcttag gtgattattg cagtagatat tacaaaaata cagaaaaaaa
     6301 cacattgatt tttgaaaata acaatagagc tttagcaaga aaaaaatcat ccatctctac
     6361 tctatttaaa ttcttataca gaaactctca gatagataat aatataactg atggattcaa
     6421 tccaataaaa cttcctaaac ctcagccaga tgctattaag cgccttgaga tagatgaagt
     6481 tgctaagatg cttgaatcag tagaaacagg tgaagggctt acagagaaag aaaaggtata
     6541 ttggagaaaa actaaattac gtgacaaagc tatactagca ttatttgtta catacgggct
     6601 tagattaaat gagcttagag aactcaatat ctcctctttt aatttttcaa gaggtgaatt
     6661 taaaatatat agaaaacgtg ggaaagaagt tttaatgcct ataaatcata cttgtgaaca
     6721 tgtaataaaa gattatttgc aaaacgaaag aacaagagat gaactattaa atgatgaaga
     6781 aaaagatgct ttatttttgt ctcttcaaaa taagagaatt acagcaaagg ctataagaac
     6841 actggtaaaa aaatatacat ctattccact tgatactact agagacaatg gatatagtcc
     6901 ccataaatta agagcaacgg ctgcaacttc tctaatccaa acaggatttt ctatatatga
     6961 tgttcaaaac ttacttgacc atgataatgt cacaactact cagctttatg cagctcataa
     7021 gaaaaatgta aaacgtgata tagtcaagaa ttttgaatgg attgaggacg attaaaaatc
     7081 aggaacaaat gtttgcagat ataaatggat tatgttataa ttaaaaatat aaagtgtatg
     7141 gaaataaaaa caaaatatgt ttggaaatga gtgatgttat gtatttagat ctaactgaaa
     7201 agcaagtctt gatattggaa tttataaagt ctcaaatcat attaaaaggt tatccacctg
     7261 ctgtaagaga aatatgtact gctgtagggt taagatctac ttcaactgtg cactctcatc
     7321 taaataaact tgaaaaactg ggatacataa gaaaggaccc tactaaacca agagctattg
     7381 aagttttaga acgtagtaaa gtaaatgatg tttctggagc taatcaagaa ataatagagc
     7441 ttcctttagt aggtcaaata acagctggag aacccatttt agctcaacaa aacatagagg
     7501 aatacattcc attccctgcc agtttagtaa agggcagtaa caattttgta ttaagagtaa
     7561 aaggcgaaag tatgattaat gcaggaattt tagatgaaga ttacgttgta gtagacaaaa
     7621 aaaatacagc cttaaattct cagatagtag ttgcacttat aaatggtgaa tctgctacgg
     7681 ttaagaggtt ttttaaagaa ggaaatttaa taagacttca gccagaaaat gattttatgg
     7741 aaccaattat gctcaatgac tcagaagttg aaattgtagg tattgtaact ggtgtattta
     7801 gagttattaa atagctatta aaaaatactt aaaaaaataa gctattcgta tagataaaat
     7861 gtgttatcta ttgattatag cttatttttt tatgcattta ttttattttt taaatgattt
     7921 aaagttcaga agctttatag ataaattggt tgtttttttg ttattttcca taacatatat
     7981 cacatgggtc atttttctta ctttctgcaa ttgtgcctga taatattttt ttacttcttg
     8041 aaagcccact acaatttttt gtagagtgat agctcttacc ttttggagta aaatacacta
     8101 tttcttctga attggatgta ttttgtttat ttttaaaaga atcattttta cttgtattac
     8161 tgtttgattt tccagagttt ccatcttctc taacgccttt attataactt ccatctttac
     8221 aacttgtaaa tactccatca cctgttgatt caaatattat atctgaacac tcatcacttc
     8281 tatgcacttt tactcccatt ttttttaatt tattcattgt ctcctgatgt ggatgaccat
     8341 atgaattacc cttgcctact aaaagaaccg catattcagg atttacttta tctaaaaact
     8401 cttgagatga actactacga gaaccatgat gagctacttt taaaaggtct actttctcaa
     8461 gcttaggtag tatttctttt tcggtacctt cttcagcatc acctgtaaat aaaactttat
     8521 cattcccatt agtatataca aggactagag attgttcatt agtagtatct cccccatttg
     8581 tatttagaac ttcaaaataa gaatcttcta aatagaactt attattttca agcggtacac
     8641 ttggggataa acccttattt gctaaagcat ttataaagtc cctataactc tttgtttctg
     8701 ctgaaccatt cgatacaaaa acattttcta tattaaagtt tttgacaaca gaatcaagtc
     8761 cacctaaatg gtctgcatga gaatgggtag ctataagata tttaatatct tttacacctt
     8821 gattgtttag gtaatctacc attaatccct catcatcatt atctccccca tctatcaatg
     8881 taaaagtttt accatcttga atcaatattg catcactatt tccagtgtca ataaaatgaa
     8941 tttttacatt cttatcactt gatatactac tagacaaaga atcattatta tttgattgat
     9001 tatttccatt atctgatgta gtacaaccag ttaaataaat cgatatagat actatcaata
     9061 aaagtatcaa agttttaatt
//
LOCUS       DAYAOX010000145         9034 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.145, whole genome shotgun sequence.
ACCESSION   DAYAOX010000145 DAYAOX010000000
VERSION     DAYAOX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.145"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            230..1045
                     /gene="yqeB"
                     /locus_tag="ACGI57_002665"
     CDS             230..1045
                     /gene="yqeB"
                     /locus_tag="ACGI57_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFG9717735.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            1271..1708
                     /locus_tag="ACGI57_002666"
     CDS             1271..1708
                     /locus_tag="ACGI57_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HFG9717736.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            1893..2120
                     /locus_tag="ACGI57_002667"
     CDS             1893..2120
                     /locus_tag="ACGI57_002667"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HFG9717737.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            2125..2511
                     /locus_tag="ACGI57_002668"
     CDS             2125..2511
                     /locus_tag="ACGI57_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFG9717738.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            2514..3218
                     /gene="atpB"
                     /locus_tag="ACGI57_002669"
     CDS             2514..3218
                     /gene="atpB"
                     /locus_tag="ACGI57_002669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFG9717739.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            3287..3547
                     /gene="atpE"
                     /locus_tag="ACGI57_002670"
     CDS             3287..3547
                     /gene="atpE"
                     /locus_tag="ACGI57_002670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFG9717740.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            3670..4167
                     /gene="atpF"
                     /locus_tag="ACGI57_002671"
     CDS             3670..4167
                     /gene="atpF"
                     /locus_tag="ACGI57_002671"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFG9717741.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            4164..4709
                     /locus_tag="ACGI57_002672"
     CDS             4164..4709
                     /locus_tag="ACGI57_002672"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFG9717742.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            4726..6228
                     /gene="atpA"
                     /locus_tag="ACGI57_002673"
     CDS             4726..6228
                     /gene="atpA"
                     /locus_tag="ACGI57_002673"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFG9717743.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            6256..7134
                     /gene="atpG"
                     /locus_tag="ACGI57_002674"
     CDS             6256..7134
                     /gene="atpG"
                     /locus_tag="ACGI57_002674"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HFG9717744.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            7147..8541
                     /gene="atpD"
                     /locus_tag="ACGI57_002675"
     CDS             7147..8541
                     /gene="atpD"
                     /locus_tag="ACGI57_002675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFG9717745.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            8544..8804
                     /gene="atpC"
                     /locus_tag="ACGI57_002676"
     CDS             8544..8804
                     /gene="atpC"
                     /locus_tag="ACGI57_002676"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HFG9717746.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT         3523 a         1067 c         1770 g         2674 t
ORIGIN      
        1 ggttcttttt tatatatttt ttctcaaaat aggaattatt gaattgataa atttctcgaa
       61 ttgagaataa aaagttttta ccaattaaaa aagattgctt attttacaag gttttataga
      121 atagtagtct attttaatgt ggcatgttag ttgcttacga ttataggtat taagtgtatt
      181 agttaaattg agaattgaat attaataata ttattgggag tggtaataca tgaagaattg
      241 tatagtagta agaggggctg gagatttagc aacagcagtt attcataaat tacacaacag
      301 tggttttaaa gtagttgcgt tagaaataga agagccatta gctataagaa ggcaaatttc
      361 tttttgtgag gctgtttatg aaaaaaaagt aactgttgaa ggtgtagaat gtatattttg
      421 caatactgaa aaagaaatag aggaagtttt atctcaacat aaagtagcct tgattataga
      481 cccaaaagga gaaaaattat ctaaattaaa tccaatcgtt ttggttgatg gaatactcgc
      541 taagaaaaac ttaggtacaa ctaaagatat ggcaccactt acaatagctt taggtcctgg
      601 attttgtgca ggagaagatg tagatgttgt tatagagaca atgagaggtc ataatctagg
      661 aagaataata actaatggta gagcagaaaa aaatacagga gtaccaggag caataaaagg
      721 tttttctaaa gagagagtta tgcattcaca gtatgatggc ataatggaaa atgtatgtaa
      781 gataggtgat atagttgaaa agggagacct actttgctat attaataatt atgaaaaaaa
      841 acatgaagtt agagcaacaa tatctggagt ccttagaggt ttattaaagg atggagcaaa
      901 ggtaaacaaa aatttaaaaa tactagacat agaccctaga aaagaagaag ttaagaatgc
      961 ttttacaata tcagataaag ctagatgtat agctggaggg gttttagaag ccatactaaa
     1021 caatgggatt tatccccaaa attaatttat attagataaa gctattaata ttttattaag
     1081 atgatataat aaatttaata gattaaagag agcatatgac gggtatttaa aatttaaatg
     1141 gtaaaaatgc tcttttttta ttggacattc aattaacatg tgtatataat ataaaaataa
     1201 gtatttattt aggttgaaat ctaataaata tataatacca gtacataagc aaagaggaga
     1261 aaagtaaatt atgagaccat catgggatga atacttcatg gaaattgcag aagtagtgaa
     1321 aaagcgttca acttgtatta gaagacaagt aggagctgta attgtaagag acaaacaaat
     1381 attaacaact ggatataatg gttctccgag aaatcttgaa cactgtgaaa atattggatg
     1441 taagaggcag gaattaaata ttccctcagg agaaaggcat gaattatgta gagcattaca
     1501 tgctgaacaa aatgcgataa tacaagctgc tcataatggt attagtgtag atggggcaac
     1561 tttgtatgtt actacaagac cttgtgtttt gtgtgcaaaa atgtgcataa atgcaggtat
     1621 attgaaaatt gtttatgaag gtgattatcc agatgatatg tctacagaat tattaaaaga
     1681 agcaggaata gaactcataa aattttagtg gataaaattt atatgacaat aaatgtcaaa
     1741 ttatgacact gaaatacaat ggtatcaaat gattaaaaga aagtatttga aaaatcatca
     1801 gattagtagt aagatatatt ttataattaa ataaatttat gagatagatt aattacatgt
     1861 tatttatttt ataataatga gggatactat ttatgagtaa aagaaataca catacagaaa
     1921 ttgcaaagtt gtttagttta ataagtcaaa taggattgat gattgtaata tcaatattag
     1981 gatgtacttt cttaggaaaa tttttagata gtaaattgaa tactactcca gttctgacaa
     2041 taatattttt attgttgggt gttggaggtg cttttagtgg tgtttataaa actctaatag
     2101 tatatacacg aaggaagtga tatattggat ataaaattac ttaaccaggt aaggtcagta
     2161 acaaaaggta tagtaatatt tgatgtagtt ttaattgtgc taatgttgat aacatcaaca
     2221 ttaagtaaac cagtattatt aggacttata tttggctcta taatagctat gttaaatttt
     2281 agattattgg cgatatcact ggaaaaatca gtgacatttc ctgctgggaa ggcacaagct
     2341 tatgcatcag cacaatatgc tgttagatta tttataatgg cagttgttct aattgcatca
     2401 gctacagtac cacatttaaa tataatcgga acagcattgg gattattgag cacaaagttt
     2461 gtcatattat caaaaaactt tataaataaa ttaaagagga aggaggcata agtatgaatc
     2521 aagcgcatta ttatctcata ggaaacttta agattagtga aacaatacta gtaagttggg
     2581 gaataatggc ctttttagcg atagcatctt attttttgac tagaaactta aaaaaagttc
     2641 caactagtaa agttcaaata ttcttagaat ttgcagtagg tggtcttgct aaaatggtta
     2701 cagatgttat gggtaaaaat acagtaaaaa gaatgccata tatgatacca tacataggaa
     2761 gcttgttttt atttttcgca tgttcaaatt taagcccatt acttggatta aggtcaccaa
     2821 caaccgactt agacacaact gctgcatggg caatgattac attttttatg atttattttg
     2881 caggagttaa atttaagggg ttatcatact ttaaagaatt ggctcaacca cttgccataa
     2941 tgttaccaat caatattata ggtgagttag ctagaccaat atcattaagt ttccgtccat
     3001 ttgggaatat tttaggtgga acaataataa tgggtctgtt ttatcaactt ttaggtttta
     3061 tatctagttt aataccagga ttatcaatac caattggaga aatattaata ccagtcccat
     3121 tacacttgta ctttgactta tttgcaggat tgttacaagc atatatattt ataatgttaa
     3181 caatggtatt tatatcaagt gcaggagagt tagattaaga aatcaactta tattaagata
     3241 aaaataaaat aatttaaaaa attaaagttt taaggaggaa ataattatgg aaacagcaat
     3301 agtagcggca gcatcagcaa taggagcagg gatagcagtt gcaacaggta taggtgcagg
     3361 tataggtcaa ggtatagcag cagctaaagc tgcagaagca gtaggaaacc aaccagaagc
     3421 taaaggtgat ataacatcaa ctttattact aggagttgct atagcagagt cttcagcaat
     3481 atatggtttg gttatatcaa taatacttct attcgtaaat ccattcttta aatacttagg
     3541 aatgtagatt tagtccttaa atattttgag ataggtttat aggtaaatca tctcaaactt
     3601 ataatcatga gatgaacttt atcatctcgt gattataagt agttatgatt tatggaaagg
     3661 aggataatta tggaaaaagc actagtaggt ataacgtggg aatttgtgtt ccaaattgtt
     3721 aatactttta taatattttt acttctgaga aagcttttat tcaagccagt attaaatata
     3781 attgaatcaa gagaaaatga tattaaaagt gatttggcag aaggcgaaaa agcaaaaaat
     3841 gaaggtttag ctctgaaaaa agaatatgag tcaaaaatca attttgccaa ggatgaaggt
     3901 caagaaatca taaaacaagc aactataaga gcagagcaga aatcagatga tatagtaaat
     3961 actgcaaaga aagatgctct tgatataaaa gaaaaagcaa acaaagatat agaacaagaa
     4021 agacaaaagg ttataaatga aatcaaaaat gatatatcta acatagcact acttgcagca
     4081 tctaaagtca ttgaaaaaga tttagataaa tctaaacatg aagaattgat agaaaacttt
     4141 ataaaagagg taggggaagc taaatgataa atgtaatagc aaatagatat gcagaagccc
     4201 tatttcaatt aggagaagaa gaaaattcaa ctgatgtctt attcaaagaa ctagaaaaag
     4261 tagtagatat gatgactaaa gttagtaaag atttctacaa agttttaaaa tcaccattag
     4321 tttcaaaatc agaaaagaaa aacttggtag aaattatttt tagtaaagaa gtaagttcta
     4381 acataaaaaa ctttttaaaa gttttagtag ataaagatag aatatcatat ttagaagata
     4441 tcgaattagc ttataaagaa ttattaaata agaaaaacaa tgtaattgat ggagtagcaa
     4501 taagtgctat tccaatgagt gaaacagata ttaaagaact tgaagtcaaa ctttcaaata
     4561 aatacaacaa aaatgttact atagaaaatg ttgttgacaa gactatacta gggggagttc
     4621 tagttagaat tggaaatgaa caaatagatg gcactgtgaa aactcgtctt gataagatga
     4681 aagaaaagct atctgaagta atatcttaag aatggcggtg aacccatgaa cttaaaacct
     4741 gaagaaataa gttctataat taaacagcaa ataaaaaatt atgagaataa agttgagttg
     4801 acagatacag gtagtgtttt aacagttggg gatggtatag cgagtgtata tggattagaa
     4861 aaagctatgt ctggtgagtt gctagagttc ccaggtgaaa tatatggtat ggcacttaac
     4921 ttggaagaag aagtagttgg agctgttatc cttggagatg acagtgaaat aaaagaaggc
     4981 gatatagtta agagaactgg aagaatagtt gaagttccag ttggagaagc tttaataggt
     5041 agagttgtaa actcactagg tcagccaata gatggaaaag gacctatagc gtacacaaaa
     5101 actagaccag ttgaatctga ggcacctggt ataatagata gaaggtctgt ttatgaacca
     5161 ttacaaacag gaatcaaatc aatagattca atgataccaa ttggtagagg tcaaagagag
     5221 ttaataatag gtgacagaca aactggtaaa acatctatag ttattgacac tatactaaat
     5281 caaaaaggaa aagatgttat ctgtatatat gtagcaattg gacaaaaacg ttctactata
     5341 gctcaattag ttagctcttt agaaaaaggt ggagctttgg attatacaat agtagtttca
     5401 gcaacagcat ctgaatcagc accacttcaa tatatagcac catatgctgg agcagctatg
     5461 ggtgaagaat ttatgtacaa tgggaaacat gtacttatag tgtatgatga tttaagtaaa
     5521 caagctgttg catatagaga aatgtcattg ttacttagaa gaccaccagg acgtgaggca
     5581 tatcctggag atgtattcta tttacattca agattacttg aaagagcagc taagttatct
     5641 gatgaattgg gtggaggttc aatgactgcc ttgcctataa tagaaactca agctggtgac
     5701 gtttctgcat atataccaac aaatgttata tctataacag atggtcaaat atacttacaa
     5761 ccagagttat tctattcagg tgtaagacca gcagttgacc ctggtatatc agtatcaagg
     5821 gttggtggtt ctgcgcaaat taaagcaatg aaaaaagttg caggaacatt aaaacttgca
     5881 tattcacaat atagagaact tgcagcattc tcacagtttg gttctgactt agatgaagat
     5941 actaaaaaga gacttgctca aggtgaaaga atcgttgaga tattaaaaca aggtgagcat
     6001 caaccaataa aagttgaaaa tcaagttatg ataatatatg ctgttataaa taatcattta
     6061 gaggatattc cgatagataa tatagctaga tttgaatcag aattgtatgc atttgtagat
     6121 aataattatc cggaaatatc aagaaaaata ttaggtggag aagacttcac tcatgattta
     6181 acagatgcaa taaatgaatt taaagaaaag tttgttgttg aagtataatc ctttttaaaa
     6241 aaggaggtaa attaattggc aggagctgga atcaaagcaa tcaaaacacg tatgggaact
     6301 gttgaaagta caaagcaaat aactaaagca atggaattag ttgcatcttc taagcttaga
     6361 aaagcaaaag aaaaggctga agattcaaga gaatatttta atactttata caatacagta
     6421 aaagatattg cccaaacaac acgtggaatt agaaatgaat ttttaaaaga aagagaagta
     6481 aaaaataaat gctatgtagt agtagctgga gacagaggac ttgcaggtgg atacaattct
     6541 aatgtattaa aggaatctgt ctctcatatg aaaggtaaaa aggaaacagt gattactgtt
     6601 ggaaagaaag cacatgaatt tttttctaaa agaaactaca atatagcaaa agatataaat
     6661 agtgttgaag cttgtggata tgaagatgcg acagaaattt cacaaactat tattgacctg
     6721 tataaaaaag gtgaagtaga tgaagtttat attgcttata caaaatttaa atctgcttta
     6781 tcacaagaag ttgaaatttt gaaattgctt ccattagtct ttgaaaaaga tacagacgaa
     6841 caaggaatgg cagtaaaaga agaaaaacaa aatagatcaa aagtacaata tataccatca
     6901 gcagaagcag ttttaagtta tattgtacct cagtttatat caggtacagt atatggtgga
     6961 attttggaat cttttgcatc agagcaaggt gctagaagaa ctgcaatgga atcagccaca
     7021 gacaatgcga atgaaatgct atctaattta gagttaagtt ataacagagc aagacagtca
     7081 gctgtaactc aagaaataac agagatagtt ggtggagttg aagctctaaa atagggaggt
     7141 taggaaatgg caaatgtagg taaagtagta cagattgtag gagctgtact agatgttaag
     7201 tttgatagtg aacaaagcct acctaactta ttaaatgcgt tagtaataaa gttaggagat
     7261 aaagaaatag ttgcagaagt tgctcaacat ataggtgatg atactgtaag atgtatagct
     7321 atgagtgcaa cagacggact tgtaagaggt atggaagttg tagatacagg agggccaata
     7381 agtgttcctg ttggagatga aactttaggt agaatattca atgttcttgg aaaaccagtt
     7441 gatgggaaac cagctccaaa atctgcacct aaattaccta tacatagacc agcaccagca
     7501 tatgatgaac tagaaactac agcagagata ctagaaactg gtattaaggt agttgactta
     7561 ctagctccat atctaaaagg tggaaaaata ggtctatttg gaggagcagg agtaggaaag
     7621 acagttctaa tacaagagct tataaacaat attgctaagc aacatggtgg tatttctgtg
     7681 ttttcaggag taggagaaag aacaagagaa ggtaacgacc tttatggcga aatgagtgag
     7741 tctggagtta taaataaaac agctctagta tttggtcaaa tgaatgaacc acctggagcg
     7801 agaatgagag ttgctttaac tggacttaca atggcagaac attttagaga tgagcaagga
     7861 caagacgttt tacttttcgt tgataatata ttccgtttca cacaagctgg ttcagaagtt
     7921 tcagcacttc taggacgtat gccatcagct gttggttatc agccaacatt agctactgaa
     7981 atgggtgcac ttcaagagag aataacatca actaagaaag gttcaataac atctgttcaa
     8041 gcagtatatg taccagcaga tgacttaact gacccagcac cagctacaac attctctcac
     8101 ttagatgcaa aaacagtttt atcaagacaa atatcatcac tgggtatata tccagcagtt
     8161 gaccctcttg aatctacatc aaggattctt gaccctagta tagtaggaaa agaacattat
     8221 gaagtagcta gaggtgtaca atcaatactt caaagatata aagaacttca agatataatt
     8281 gctatacttg gtatggatga attatctgat gaagataagt taatagttgc acgtgctaga
     8341 aagatacaaa gattcttatc tcaatcattc acagttgctg agcaatttac aggaaatcca
     8401 ggtcaatatg ttcctgttaa agagactgta agaggattta aagaaattct tgaagggaaa
     8461 catgatgact taccagagtc agcattctta tttgtaggaa caatagaaga cgctgttaga
     8521 aaagcgaagg gaagtatgta gtcatggcca gtgaattttc attgaaagtt gtaactccaa
     8581 gtaatacttt ttatgatgga gatgttgaaa tgatagtagt tagaactaca aaaggagaca
     8641 aaggggtgtt gaaaggtcat ataccatttg tttcaggtat acctgtagga actttaaaga
     8701 taaaaaaaga gggaagtttt aaagaggcga aaatagcaga aggattcatt aatgtaactc
     8761 aagataaaac tgtcatattg acagaatctg ctgagtggat ttaataaatt aatttagagt
     8821 ataaaaagtc attttgacca aaaaaacttt tgggtaaaat ggcttttttg cgttttggtg
     8881 ttaataaaat aacgataaaa ttgtaagttt ttagtgataa taaattcaca aaatgtttaa
     8941 atttactgcc aaattatgtt gtaattaata agataaagaa gatttagata gcttttatgt
     9001 aagtgaaaaa gtcttcatat aagaaataca tata
//
LOCUS       DAYAOX010000146         8999 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.146, whole genome shotgun sequence.
ACCESSION   DAYAOX010000146 DAYAOX010000000
VERSION     DAYAOX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.146"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(152..1597)
                     /gene="purB"
                     /locus_tag="ACGI57_002677"
     CDS             complement(152..1597)
                     /gene="purB"
                     /locus_tag="ACGI57_002677"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFG9717747.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(1741..2649)
                     /locus_tag="ACGI57_002678"
     CDS             complement(1741..2649)
                     /locus_tag="ACGI57_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9717748.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            2906..3730
                     /locus_tag="ACGI57_002679"
     CDS             2906..3730
                     /locus_tag="ACGI57_002679"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFG9717749.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3770..4300
                     /gene="lepB"
                     /locus_tag="ACGI57_002680"
     CDS             3770..4300
                     /gene="lepB"
                     /locus_tag="ACGI57_002680"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFG9717750.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(4382..6163)
                     /locus_tag="ACGI57_002681"
     CDS             complement(4382..6163)
                     /locus_tag="ACGI57_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFG9717751.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(6545..8881)
                     /locus_tag="ACGI57_002682"
     CDS             complement(6545..8881)
                     /locus_tag="ACGI57_002682"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9717752.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
BASE COUNT         2982 a         1369 c         1070 g         3578 t
ORIGIN      
        1 tattatttat atagctattt tattaaattc atggtatagt aagtaaagaa gaactcttca
       61 cagcggtgga gagaagacgc tagcccatta aaatggctat cttatttata aataagatag
      121 ccgtttttaa ttatatttta tctgtacaat tctatactct taggtctaca tctacttttc
      181 ctaagtcttc cttataatct tttatagcag gttctacaac ttcatttaca aattctacaa
      241 cttgttctgg agctctacct ataaagtttt ttgggtccat tatagatagt atttcttcct
      301 tgctcatttt aaatgaatca tcattcatta taagttcaat taagttatta tccttgcctt
      361 catgctttac tctataggca gctttcatag aatattctct tattatttca tgcaattcct
      421 gtctatctcc acctcttttt acagcttcca taagtattgt ttctgtagcc ataaaaggaa
      481 gttcttcatt tacacgtcta tttatcatat tttcatatac tactaaacca tcagttacat
      541 ttatccctat ttcaagtata gcatctgctg ccataaatgc ttgaggtatt gccaatctct
      601 tattggctga gtcatctaat gttctctcta accattgagt ggcttgtacc attgcagggc
      661 ttatagattc agagattata tacttagata aagatgctat tctctcactt ctcattggat
      721 tcctcttata agccattgct gatgaaccta tttgattttt ttcaaatggc tcttctaatt
      781 cttttaaact ttgtaaaagt cttatatcat tagtcatctt atgcatactt tgagctatcc
      841 ctgatagtat gcttattact tgataatcta actttctagt atatgtctgt ccacttactg
      901 cataggaaga atggtatccc atctttttac aaattttctt atctaactct tttacttttt
      961 cgtggtctcc ttcaaataag cttaaaaaac ttgcttgagt accagtagta cctttaactc
     1021 ctcttaactt catgttgtct attctatagt ttaaatcttc caaatcaaga attaaatctt
     1081 gagcccataa acaagctctt tttcctactg ttgtaagttg agcagcttgg aagtgtgtaa
     1141 atccaagagc tggcatatct ttgtacttaa gtgcaaattc ttttaaattg tttattaaat
     1201 ttacaagctt tactcttaga agttttaaag cttcattcat ttgtataaca tctgtattat
     1261 ctccaacata tgcagatgtt gctcctaaat gtattattcc tttggctttt tcacattgta
     1321 aaccataagc ttttacgtga gacattacat catgtcttac ttctttttct atttttctag
     1381 cttcatcaaa atttatatta tctatattag ctcttagctc atctaattgt tcttcagtta
     1441 tattgattcc taactcatgt tcaccttctg caagtgcaac ccataacctt ctccatgtag
     1501 agaatttaaa ttgtggagaa aatatataac tcatgtcctt gctacaatat ctgtctgtaa
     1561 gtgggtttga gtaagtagta tatttattat ctatcattat attagcacct tccttaaatc
     1621 ttttgtcgtg tgattactgt gtacacatta gtacataaat aattatatat aattttacga
     1681 actataacaa gactttttta ttttttgttc acatttatat atagtatttt ttatttatta
     1741 ctatttttta tttatatcca aattatcttt ttcaattaag taatccacta tttcactatc
     1801 aacaaaattt aatacatact tatatctaat tgcatagtat ttatcactcc atccaagctg
     1861 attttttaca tcactttcat ctgccccaag ccttaaactt attgctgcaa atgaatgtct
     1921 aaaatctctt gctgaatatt gagaaagtcc agctaaatct aatgcttttt tgactataag
     1981 tctgacattt ctatctgtta tagaattact cttttgagtt gtaaaaataa aatcatcact
     2041 aggtattatt acctctggaa gcccagaata attcctgtaa tcttctaaaa gtttaaaaaa
     2101 gtatggatgt aatttaacta ctctttcttt tcttccctta cctaacctta catatgagtt
     2161 atcgttttca tctaccataa ggtctttcca ctttaagcta actaattcat ttaataaaca
     2221 cccagtagta actagacaga ctattattac tctatctctt atatttaatt ttggtaggat
     2281 acctataagt ttatttattt cttgtatact tagaacatca tcttttgttt ttattcttgt
     2341 tactgtgggt ttttctacat atctaaatgg atttatatct atgtatcctt ctttttttaa
     2401 gaagttataa aaactatgta aataactata tatcttttca caagttgatt tcgcatattt
     2461 agtctgtata tagtctacaa aatttttaca atcttctttc gtagcataaa tcaattcttt
     2521 cccttttaaa aattctttaa acagtataac ctttgataaa taatcgtgtt tcgtgtttgg
     2581 atttaatttt gtagaaaaaa cattaaaagt ttcatactca taatcatcta gtatttttgt
     2641 actatacata ataactcctt tccttttcat aaaataaaat agctatactt ttactatacc
     2701 atatatttcc ccctcaaata taccccttat tcaatagata acttttaagt ttattttgtt
     2761 atattatgaa ggttttatga aaatatcaat cttccattgt gcactacaca gtactatggt
     2821 attataagtt agtaaaaggt ggcatttttt tattaatcta tgaataaaat atgctactat
     2881 aaatttgtat tggaggtatt ttgatttgga aacatttagt ttgcttgaaa tttttaaggc
     2941 agtcattttg ggcatagttc agggaataac agaatggcta ccagtaagta gtacagggca
     3001 tatgatatta gttgatgagt ttataaaatt gaatttttca aatacattta taagtacatt
     3061 tttagttgta attcaatttg gttcaatatt agctgtactt gttatatttt ttagaaaatt
     3121 aaatcctttt gatagcgcaa agaatattaa gcagaaaaaa gaaacagtcc gtttatggct
     3181 taaggttata attgcagtta taccatctgg agttattgga atactgtttg aagatgatat
     3241 agatagatta ttctttaatt caacagtagt tgctattgct cttattgtat atggtataat
     3301 aatgataggg cttgaaaaga gaaataaaaa acctaagtat aaagattttt ctcaagttac
     3361 atataaatta gctctttgta taggattatt ccagtgtcta gctcttatac caggtacatc
     3421 taggtctggt tctactataa taggtgcagt attacttggt acttctagat atgtagcagc
     3481 agagttttca ttcttcttag ccatccctac aatgcttgga gctagtgcat taaaacttct
     3541 aaaagctgga tttggattta ctggatttga atggcttatt ttaggtgttg gttctgtagt
     3601 agcatttgtt gtttcaattg tagttattaa attttttatg gactatatca aaaaacatga
     3661 cttcaaagta tttggttatt atagaatagt cttgggtata gtggttcttg cttatttctt
     3721 tctattataa tgtattctaa tttaataaca aatgataagg agaaaagtta tgagtgttaa
     3781 aaaagaaata tttgattgga ttaagtcaat agctatggct attgtacttg catttgtaat
     3841 tctacaattt ataagacctt ctattgtaag tggagaatca atgtatccta ctttagatga
     3901 taaagattat ctgattttaa ataggatatc atacaaggtt ggtaaacctg aaaaaggcga
     3961 tattgtagtt tttaaaacca atttagttga tggagaaaca ggaaagaaaa aagacttaat
     4021 aaaaagagtt atagctactg aaggtgacag aataaaaata tcaaattcta aagtgtatgt
     4081 aaatggaaaa ttattaaatg aaccatatat acacaataac tatacttctg gagatataga
     4141 tactgttgtt ccaaaaggta aactatttgc aatgggagat aatagagaaa atagtaatga
     4201 tagtagattc cctgatgtag gtatggttga tgaagatgaa gttcttggta aggttatggt
     4261 gagactatta cctcttgata atattgggaa agtagactaa aaaataagag actgaattgg
     4321 taaagtttat tttacaaatc tcagtctctt tttattttct taaattacaa atattttttt
     4381 actaaatatc ttttgattct gtaataagtc ttattatatc acttgcttct tcattaaaag
     4441 ttgttactat acttaggtag tctgttgctc tactcatagc taaataaacc ttatttaaat
     4501 ctcctacaaa gtcatttact tgataatctt gttcaatatc atttatagtt tgattgtata
     4561 acatctctaa ttcacatact attaccgctt tatactctaa attctttatt gcatatatat
     4621 ttgctattgt agttcctacc ttcttactta tatttgtcaa attatcttct gcacatatat
     4681 aaggaatttt tgcctcttcc aatgcttttt ttaacatata ttgaaagtaa atggtttttc
     4741 cactttttaa tttcttctta ttgtaaggat aaacgatagc aacttcagaa taatcaagac
     4801 cttttttact tattaaatac tcaatttccc agattacaga acttatttgg tcatcaaggt
     4861 cacttacttt tattatatca acagacttat ttccacctct aagtgcttta gtataaaaga
     4921 aagtactaaa ttctgtattt ggtcttaatt cgttgatata tgagttagaa ttatttgaaa
     4981 atttatttgt aaaatctaca atctctttag cttgtctata atttttactt aagataatct
     5041 tatcatcaaa ttctatacca ttatataatt ttttaaatat attcaatcta ttagagatat
     5101 tcaaggagtt acaagaacac acattaaata tgaattttgt tttatataaa aactccctta
     5161 taaaatctat ttcatactct aaaaagcttt ctgcttcatc aataaaaata gccttgaaca
     5221 tatttttatt ttttataatg ttctgtgctt gttttacaag attattaaag gtcttttcgt
     5281 aatcattttt taacatgtta taatctacaa ttaaattgaa ttttttagct agtttaaata
     5341 taaaagaact aaatgtgtga acttctaaat tgttattatc tttgtataat aactctatcc
     5401 tctctcgaag ctcattgcac agttgttttg tatgtgtaaa tatgactaat ttatgatgtg
     5461 gatatacttt tgctaatttt atagccctag aaagcataat tgctgttttg cctataccag
     5521 aaccaccttc aataagtgta ttcccatatt taatagaaac aacatcttta atttgaacgt
     5581 cttccatcat agttgctgta tacttatagt catcattgta gaatgaaatt tttttaaatt
     5641 tttcatttag atgaagttta tcatttatta tatagtattc tgaacatatg tctagtaaaa
     5701 ataaatttaa gtttatttca tcattctcat cattaagata ttcctccagc gaacccttat
     5761 tcattatatc ttgaagctta tttttatcta ttatattatt attaacaaat tcttcaaatt
     5821 catatgtttc ctcaacttca acgtatggca taatgtaaac gtagttataa cttatatttc
     5881 tattcttttg tcccattttt agtttcaaaa gttcatattc ctcattcata acttcaagta
     5941 actcttcttc taaaataaaa aatatatctt ctgttgtatc cataaatttt ataaataaaa
     6001 ttcttccatc tttaacatac aacaaatcgg tatttattcc tttaaaagga gtaacttttg
     6061 gcactacttt aacgtcctca ttgatatttt cgtaaaatat tttctcgaac tccttcaatt
     6121 ttagggcgtc taactgagaa tttatattta tgtattggct cacttacatt acctccttct
     6181 gttatactta attataccat tttgaactat atttttatat atacttttta taggcaaatt
     6241 tagtaaataa attcaaaata aatacaaata aatacaaaac tgctaaaaaa attttgcaat
     6301 aagtatagat atttattgtt ataaaatact ttctgtaaat aataaaatga taaatttcat
     6361 aattaatgct taaaataata agtaaagtct taaaaaaaat aaatcttgtt agccaaaaat
     6421 aagatttatt ataaaaaatt taaaaacctg ctaaaaataa tctattttca ttagcaggtt
     6481 ttatttataa tcttcttata ttaatactat tatatcctat ttttagattt tactgtagta
     6541 tttactattt attttctagt aaagtattga tacttgatat gtctgtataa atcgcataaa
     6601 aaacaggata acgattttga tataattcac ttacaaactt accttgcatc cttacaggaa
     6661 aaatatgacc atctgtatga caaaaacgaa aaactatttc aaaagtctct cttttagaaa
     6721 ttgcttctct taatgtatct agtaccattt gtaagtcgtc ttgtaataca ggcgaaaaaa
     6781 tatcaaaacc tatagaagta acagtttgag ctgaatatcc agtaaattca agcatttttt
     6841 ggttgaaata aagtaatttt aagttttcca tacaagcaaa tttgacaaca ccatcagtag
     6901 ccatttctaa taatactttt atatctctag agtctatatg aaatgaaata ctatcaatag
     6961 aatcctctga aaatatatga taagaatttt tcccatggct ttttgcatag taaagtgctt
     7021 ggtctgcttt ctcatatagt tcagagtata catatccatg ttcaggatac attgctacac
     7081 caatactaca tgcaatagaa aattctgctt gctcaacaaa ttttttctga ttaatcttag
     7141 aacaaaaacg ctctaatttg tctataagca catagttatt attattaaag tcttttatta
     7201 atactacaaa ctcatctcca ccaatacgtc ccacaatatc gtcctcacga aattgagatt
     7261 ttaattctaa ggcaaattca cttataacaa agtctccaaa tacatgacca tatttatcat
     7321 ttacattttt aaaattatca atatcgaaaa ttagaaaaac gtgttctaaa tcttgattac
     7381 tttcattttt taaaaatgaa cgaataaatt cctctgttgt agctttattg taaagccctg
     7441 taagagaatc acgttgtgaa cgttcgagaa gtagtaattc tctatttttt tcttcatcta
     7501 tatttttacg ataagatatc atacgaacag attcatctga tgtccaaaag aaaattcgtg
     7561 cattaattct aatccaatga taactttctt catctttttt aattaaaaaa tcatagctga
     7621 tattattaat tccgctatta taacgtttaa ttacattttc aggtaaaaat gtatctaaat
     7681 aaccttgtgc atattcttct ttgatttggt ttttagctat tgtatgtaag gcttcatcat
     7741 atggggtatt aagccctaat ccaaattcat gaaaatacct attctcatct tcacaaccag
     7801 ctttattgtt agtaatatca atttcaaaaa tattttcata aagaccttca gtagatttgc
     7861 tcaacaattt ttgatattcc aatttctgtg aaacactaag ctttattatt tggtttttgt
     7921 attttttaag cacatgtgta atagtaaaaa gtacaaatat tataattaaa actataactg
     7981 caatgccttt aaaaaattgc agatgaagct gtttaatcat taaagtaaaa tcattttcta
     8041 gaacaagatg ccattttaaa ctaggtatgt actgacatac aatataactt tttgattttt
     8101 cagatgaata ccaaaaactc ttttgttcct ttttgttgtt taatattaat tgtttcgaat
     8161 tagacaagtc gctactagaa ttttcaaaaa aatttatatt ctcatgaccg gttttatcta
     8221 ctgctaattg tacaaaccct ttatcatcaa ttaagcgtgc aacaacatca aatttgtcat
     8281 tatatccttt aagcaacatt tgtaatgaat taacctttaa cccaacacct ataattccca
     8341 tagttgctcc attatcatct tttatcttac aatttacaaa gactgtaata ctattgttac
     8401 ttgcttcatc attatcaaca tttaatgagt attcatcatc attctttaag aaagtataat
     8461 accattgatt ctctgagtta ttagcgctaa gatttctatc aagtccatta aaatgataat
     8521 aattattggt tttagttgat actaaaaaaa cagaatcata tgaatattta tttctataca
     8581 cattcagata atcttgtaat ttataaataa attcttcatc atttaaatgt tctttctcac
     8641 catctaaaaa attttttaat agactgtcat ttgccattgt aagagataca tttataggtt
     8701 ctgaaagtag tttatctatc tgataataaa taccttcagc agccaatgta gatacatgtt
     8761 caatatcatt tttaaaaata ttggtattag attgatatcc aattatagag gtagccacaa
     8821 aaccaagagt aataataatg caaaccataa tatttgtatg taaaagtgta ttttttttca
     8881 tagtgtaccc tctctctaat cctcacttta gcgtcattgt attttcatct tatttaagta
     8941 caagcattcc aagtagtaat atatttattt tacaacttca ttatatcaaa caaacttgg
//
LOCUS       DAYAOX010000147         8952 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.147, whole genome shotgun sequence.
ACCESSION   DAYAOX010000147 DAYAOX010000000
VERSION     DAYAOX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.147"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            74..415
                     /locus_tag="ACGI57_002683"
     CDS             74..415
                     /locus_tag="ACGI57_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFG9717753.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            566..979
                     /locus_tag="ACGI57_002684"
     CDS             566..979
                     /locus_tag="ACGI57_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HFG9717754.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1181..1822
                     /locus_tag="ACGI57_002685"
     CDS             1181..1822
                     /locus_tag="ACGI57_002685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFG9717755.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1846..3099
                     /locus_tag="ACGI57_002686"
     CDS             1846..3099
                     /locus_tag="ACGI57_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFG9717756.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3145..3795
                     /gene="cmk"
                     /locus_tag="ACGI57_002687"
     CDS             3145..3795
                     /gene="cmk"
                     /locus_tag="ACGI57_002687"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFG9717757.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3846..4442
                     /locus_tag="ACGI57_002688"
     CDS             3846..4442
                     /locus_tag="ACGI57_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFG9717758.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4455..5294
                     /locus_tag="ACGI57_002689"
     CDS             4455..5294
                     /locus_tag="ACGI57_002689"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFG9717759.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5539..6693
                     /locus_tag="ACGI57_002690"
     CDS             5539..6693
                     /locus_tag="ACGI57_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HFG9717760.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            6999..8696
                     /gene="ade"
                     /locus_tag="ACGI57_002691"
     CDS             6999..8696
                     /gene="ade"
                     /locus_tag="ACGI57_002691"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFG9717761.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3666 a          859 c         1579 g         2848 t
ORIGIN      
        1 catatatttc cgatagatta tattgataaa aaacactaaa attaaattaa taaaagtact
       61 tggagggatg gaaatgaaag aggaaaattt tattaatgtt gaatacgagg ataaacgttt
      121 ttcaattaat agtaaagaag ttttgtacat agaaaagcaa gagtatgaag taaagattta
      181 tactgaaagt ggtaattatg aagttggaga aaatgctcct tgtataagaa aactattgaa
      241 agaagaaaat tttttcaaat gcagtaatag ttatattgta aatctagaca agtgtgaatt
      301 ttatctcagc aataattgta tttttgtgaa taataatttt ataagaattt taggttcaga
      361 aagaaaaaag aaattaaaaa agaaattaga aaaattatat gattatgaag attaatattg
      421 ggaggctgta ttttatacag cctctttatt tattgattgt attattaata aattttaaat
      481 tatgataaaa tataaaacaa gtaacttatt tcagaataaa atacttttta aaagaaaggg
      541 acgagatatg ggagattata ataaaatgct tgaattaaag aataacttag gtatcagtga
      601 aagaaagtta aaatatcctt gtatctataa acatttcaaa gggaaatatt atgctacaat
      661 gggtcttagc aaggctattg atgatataga aaacatatgt gaaatatatg gtaaagaaaa
      721 tttaatccaa aacagaaata aatataaatt agttattaga catactgaaa gagaagaaga
      781 tatctatgtt tatagggatt tagatggaaa cttttatcat aaaaaagaag aagatactaa
      841 tgatttagtt ttatacaaga ccttatatga tgatacagga atatttgcaa gacctttgga
      901 tatgttttta gaaaaagtag atacagataa gtatgtcaat tcaattcaag aatacagatt
      961 tgaagaagta tataaataat ataagagaaa tatgtttata aatttagaaa aattctttaa
     1021 cagtacaata atttgcagaa atacaaatga gatttattgt aaataatatg ttagattaga
     1081 ttataaaata gaaacaaagt aaagtattat atgatataat taagttttga gaagtgataa
     1141 tttatattta ataaaaaata ggcatatatt aggaggacat atgggaaata ctttttatat
     1201 agtaagacat ggacaaacgg attggaatat attgggaaaa acacaaggtc atggtaattc
     1261 tgaccttacg cctcaaggta tagaacaggc aaaagaactt tctgaagata ttggcaagta
     1321 ttcaattgat tatattttta gtagcgacct tgggagagca atgcaaacag ctcaaatttt
     1381 aggagataag ctcaatattg aagttcaaaa aactgaggca cttagagaaa tgggattcgg
     1441 agtttgggaa ggccttttaa taaaagaaat tcaaaaagat tattcagata tatatgcaac
     1501 atggagaaat gaaccacact tagtaaatat accagaaggc gaaactctta agattataaa
     1561 ggaaagagta gatgctttta taaaggaact aaatgaaaaa tatgataata aaaatataat
     1621 tcttgttaca cattcaatta cattgagggt tatgttgttg tcatttttag aatcaggtat
     1681 ggaaaatata tatagaatta agcaagataa tactgcttta aatatagtag aatttaaaga
     1741 ttatgggcct gttatagtaa aaatgaatga tacaagtcat ataaaaaatc atgtaaagat
     1801 aaataattct gcactggagt aggaatcaat tataaggaga aatttatgag taaagttata
     1861 gtagttggtg gaggagcttc tggaatgatg gcagctttgt cagcttcaaa aaacaataat
     1921 gaagtaatat tagttgagag aaatggagaa ttaggaagaa aacttagggc aactggtggc
     1981 ggtagatgca attttaccaa taatagagag atagaagatt tttttgataa agttgtcagt
     2041 aataaaaaat ttctctatag tagtttttat acatttacaa ataaagacct tatttcgtat
     2101 tttgaaagta gaaatttgga atataagatt gaagaagaaa atgaccacaa agtatacact
     2161 aaaaatgata aatctataga agtaatagaa gtattaaata aagatttact caatcacaat
     2221 gtaaagataa tgtataataa gaaagttata gatattataa ctgaagaaat tgctctaaaa
     2281 gatgattcta ataaggacaa gtcaaagtat ctaataaaag ggattatttt agataatgga
     2341 gataaaatac taggagacaa ggttattata tcaactggag gagtgagtta ttcaaagacg
     2401 ggttcagatg gttcaatgta taagattctt aaaaagcatg gacatacatt aaacaaacta
     2461 tatcctgctt tagttccact tacaatagag gaaaaatgga ttaaagattt acaaggaata
     2521 tccatgaaga atgtggaaat atcttgtaag attaagaaaa ggaaaatatc aaaatctgga
     2581 gacatgctat ttgcccattt tgggataaca ggaccttgtg ttcttataat gtcttcttat
     2641 attaacaaga ttattgaaaa ggaaaaggta gaattaaata tagatttctt accaaattta
     2701 agtactgatg aaatctctag tataatcaga gcatttccaa ataaaaatgt tctaaataat
     2761 cttaagcaaa tactacctca aaatttctta aaagagattt tttctctatt atctttagtt
     2821 gataaaaagg caagtgattt gtctaaggca gatgaaatta gaattattga gtatataaaa
     2881 aatatgaagc taacttgtaa tgggactaca ggtataaaca ctggtatggt tacttctggt
     2941 ggtatttcag ttaaagaaat taattcttct actatggaat ctaaattagt taagaactta
     3001 ttttttacag gtgaagtaat tgatatagat gcagaaactg gtggatataa tttacaaata
     3061 gcattttcaa ctggatatct tgcgggtata agtgtataaa tataattttt taataattaa
     3121 atatgaataa atagaggtgt atatatgggt aatttagtta tagctgtaga tggtccagca
     3181 ggagctggaa aaagtacaat agccaagata gtagcaaaaa agctaaatat aaattatata
     3241 gatacaggtg ctatgtacag agcagttact tataagtgtt taaaaagtgg tattgatgtc
     3301 aataatgaaa aagaagttat tcaaatagct gaaaattcgg atatagattt taaagataac
     3361 aatatatatt tggacaaaga agttataaat gaagaaataa gaacaataga agtaagcaat
     3421 aatgtttcta atgtagcaaa aataaaagaa gtaagacagt tgatggttga agttcaacga
     3481 aaaataggaa tgaaaaattc agtaatacta gatggtagag atataggttc atatgttttc
     3541 ccagatgcag attacaaatt ctttttagta gctactccag aagaaagagg aaatagacgc
     3601 tataaagaac tttgtaataa aggatataat actactttag aagaagtaat tgaggatata
     3661 ataagacgag atgaaattga ttcaaataga gaatttgcac cattggtaaa agctaatgat
     3721 gcactagaaa tagatactac aggaaaaact atagaagaag ttgtagaaga agttgtatct
     3781 aaaataaatc tttagttttt tatgcatcat aatgaaaata aatgtagtgt tgaagggaga
     3841 taaacgtgaa tttttataga tttgtaataa atatttttaa gggtttttct aaaatatttt
     3901 ttaaatatga agttataggt gcagagaata ttccagatag aggaaatata gtaatagcat
     3961 caaatcataa atcgaatctt gaccctatat tcttggctgc tgccatagaa aatagagaaa
     4021 tagctgcaat tgcaaagaaa gaattgttta aagtaaaacc attgggattt atattaaaaa
     4081 aattgcatgt tatgcctata aatagagaaa aaccagatgt atctacaata aaaactattt
     4141 taaggtcagt tagagacggt tatgtattag gaatatttcc agagggtact agaataaaag
     4201 gagattcttt tggtaaggct aaggcaggtc tttctgtatt tacaataaaa agtaaatcta
     4261 aagttgttcc agtatcaata atatctaaat ataaactttt tagtaaagta attgtatata
     4321 ttggagaacc aatatcattt gaagaacatt ttaaggaaaa attgagtaat gatgaccatg
     4381 aaaggatatc acaagagatt ttagaagtga taaaacaaaa ttactttaaa tattcaaaat
     4441 aagggggtaa agttatgaac ataaaaatag caaaaaatgc tggcttttgt tttggagtaa
     4501 aaagagcaat gaaaatggca tgggatgaag ttgaaaaaaa tgattctggc atatatgctt
     4561 taggtcctct tattcataac aagcaagcag tagcaaaata tgaagaaaaa ggtcttaaga
     4621 ctgtgaatga aattgataca ataccaaatc atgaaaatat gataataagg tctcatggag
     4681 ttcctgaaaa tatatataag gaagccaaag ataaaaaatt aaaaattgtg gatactacat
     4741 gtccatttgt aaagaaaata catacagtag taagtgaata tcataataaa ggctatgaaa
     4801 taatagttat tggagatatg aagcatccag aagttatagg tataaatgga tggtgtgaaa
     4861 attcagctat aataataaag accttagaac aaatggaaaa tatggaattt gataactcca
     4921 aaagatattg tcttgttgca cagactacaa taaatccaga gttgtatata agtatagtta
     4981 ataaactatc agataaatta gaagaaatag tatttaatga tacaatttgt tcagctacaa
     5041 aaacaagaca agaatcagct aaagagttag ctaaagaagt tgattgtatg atagttatag
     5101 gaggtaaaca cagttcaaat actcaaaagt tagtaaaggt ttgtgaagac ttagtaccaa
     5161 cttttgcaat agagactaaa gatgaattgg atgtaaatac gcttaaaaaa tataaaaatt
     5221 tgggtattac agcaggggca tctacaccta attggataat agaagaagtt gtaacattcc
     5281 ttgaaaattt ataatttgat aggtaataaa aataggtgga cattgttata atttatcata
     5341 atatccacct ttgatttcat attacatgtg gtaaatagat aacctaatat caaaaagtgt
     5401 gtatcttaag atgaaaaact aagagtataa gatgcgcact ttttaatatt agaaattcaa
     5461 aagaaatatt gatattgatt atcaatagaa aatagaatat aatataattg ttgcaaaatt
     5521 agtctaggag gtaaattaat ggaaatgttg atatccgctt ctgaagaggc gtttttacat
     5581 gtaggttcta tgattggatt ttttattttg ttatttggat atattaatta taaaactagt
     5641 ggaaatttta ctaatatcat atcaaaaaac agaaaatttc aacccttaat aggtgcttta
     5701 attggagcta taccaggttg tggcggaagt ttggcaataa tgccattgta tataaatgga
     5761 aaattaagct ttggagcaat aattgcaagt ttaatagcta gtatgggaga tgcagcattt
     5821 gtacttattt catcaaatat taaaatgtat ttttttgtca ctatagttag tacaattaca
     5881 ggaataataa ctggacaatt agtggattac tttaaactag aagaaaaatt agggttaaaa
     5941 aaccgtagaa aatcagataa atatgatata agtaaaaata accctaaaga aaataaagaa
     6001 caacataatc atgatgaaat aattttagac actctagcta aatctcatgg aaatacaaat
     6061 agacttgcat ttataataac acatggaaag ggatataaaa tttatatagg aattatagta
     6121 atcggtttta tatttatgtc acttgctcac tctggactta atcttcctat aattgagaag
     6181 ttacattcat tggaagaagt aatagcagtg attggaatat tattttcaat aatttatatg
     6241 tggtgtttta aaaaggtatt taaaaattct aatcaaaaag aggaagaaaa taaaaagatt
     6301 tctttgcgag aaatgcttat ccattctgtt ggagaaatat catttgtaat tacatggatt
     6361 tttattgcat atcttattta cgatttgata atattaattt taggtggaga tgaatactta
     6421 gttaaattag tattatcaac aggtgtaata agtgtattta taggtgcagg tcttggactt
     6481 attccaggtt gtgggataca aattgtgctt atgtcatttt atttgaaggg aactatacca
     6541 cttggtgcag tgatagccaa ctctatttct caagatggag atgcactttt cccactttta
     6601 gcaatggata aaaaatcttc tttatgggct atgattatta ctactatacc agcaatactt
     6661 gtaggtatga tagtttatgc atttttaggt taaatataat tatgaataaa gaagtattaa
     6721 aaaaataata aagcacataa atttatttat aagaatagtg aagattaaat ataaaaataa
     6781 aaaattcaat atgaattaaa ttattgctta aatattaatg agaacatggg agataatatt
     6841 attcctcatg ttctctctat ttaattaaag gaattatgga acaaatttca agtgatactt
     6901 tctatcaaaa ctcatatata atatatatta ataaattaaa ctataatacg taatacagtc
     6961 agtatattga tataaaaaac ttaggaggag ttgtaaaaat gggtaattta aaaagacagt
     7021 gtgatgtttc aagtggaaga gaaaaagctg acattgtact aaaaaatggg acaatcataa
     7081 atgtatttac agaagagtta ataacaggag atgtagcaat agtaggagac actattgttg
     7141 gaattggcga ttacaagggc aatgtagaaa ttgattgtag taacaaatat atatcacctg
     7201 gctttattga tgcacatatg catattgaat ctacaatggt aatgccaatt gaattatcga
     7261 aaaaattatt aaaatcaggt acgacaacta tcatagccga cccacatgag ttggtcaatg
     7321 taaaaggagt tagtgcaata gattttttat tagaatcgac aaaagatata ccacttaatg
     7381 tatatattat ggtgccttca tccgtacctg caacaagttt tgaaactaat ggagttggta
     7441 aatttagtgc aaaagacatg gagagttatg taaataatcc tagaatttta ggtttaggag
     7501 aggtaatgtg ttttaatgat gttataaatt ctgaaaatga aatcttggat aaactagagc
     7561 tttttaaaaa taaagttgta gatggacatg cacctaatat aaatggtaaa tcacttcaga
     7621 catatgtatg tgcaggaata gaaaatgacc atgaatgtat aacttttgat gaagtatatg
     7681 aaaaattgag agcaggatta aaaattttaa taagagaagg aagcgcagca aaaaatttaa
     7741 agtctatagt aagtggtatg ctaaagcata acttgccaat tgaagaattt atgttttgca
     7801 cagatgataa gcacttagat gatatagaaa aacaaggtca cataagatgg aatataaaat
     7861 gtgcgattga tttaggtatg gaacctgtta gggctataaa agttgcaact tataattctg
     7921 caaaagcata tgggttaagg aaaattggag caataggtgc gggttataag gcagatattg
     7981 ttgtattgaa tgatttagac aaaatggaag tggatagtgt atataaagat ggtaatttgg
     8041 taaacgaaga gatgttttct aattacaatt atgagattaa agataaagaa ttgcttaata
     8101 cagtcaagtt taaatatatt aataaagaaa aaatacagtt aaaagtaagt gaaaaaaatt
     8161 atgtaatgga gattgtacct tatcaaattt tgacaaataa ggtatatgaa tctttaccat
     8221 gtgcagatgg atattttgtt ccaaataaag agtattcaaa actttgtgtt gttgagagac
     8281 atagaatgac tggaaatgta gcaattgctc cactaaaagg atttggcata aaaaatggag
     8341 caattgctac tactgtggcc catgattctc ataatattat agttgcaggg gataatgatg
     8401 atgatattct agtagctata aattacttaa aggaaataca aggtggttat gtaattgtat
     8461 caaatggaaa agttcttgca cacctaagtc ttcaagtagc tggattaatt agtactttta
     8521 cagctgaaga agtacaagaa attactgata atatgttgga gatagcaaga aaaatggaag
     8581 ttccagagta tgtagaccct tttattacac tttcttttat ggcacttcca gttgttccac
     8641 aaataagact tacagattta ggtctatttg atgttgagga atttaaattt atatagatag
     8701 tataaaagta ataataaatg aggctttctc aaaatagaga taagtctctt tttattgttg
     8761 aaatgatgaa aatatatttg atttcataca aaaaagagca tctcatgaac taaaaatttt
     8821 atttttagat atccttattt taattaatct atcatttaac attgttttaa gcatagatat
     8881 cttaataaaa ctattttcag tctattttaa catataaata catgaaaatt atatatttat
     8941 gaaactttat ac
//
LOCUS       DAYAOX010000148         8859 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.148, whole genome shotgun sequence.
ACCESSION   DAYAOX010000148 DAYAOX010000000
VERSION     DAYAOX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.148"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(70..282)
                     /locus_tag="ACGI57_002692"
     CDS             complement(70..282)
                     /locus_tag="ACGI57_002692"
                     /inference="COORDINATES: protein motif:HMM:NF024570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HFG9717762.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLD"
     gene            complement(375..932)
                     /locus_tag="ACGI57_002693"
     CDS             complement(375..932)
                     /locus_tag="ACGI57_002693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717763.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHISSR"
     gene            complement(1291..3201)
                     /gene="phnW"
                     /locus_tag="ACGI57_002694"
     CDS             complement(1291..3201)
                     /gene="phnW"
                     /locus_tag="ACGI57_002694"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFG9717764.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(3480..4265)
                     /locus_tag="ACGI57_002695"
     CDS             complement(3480..4265)
                     /locus_tag="ACGI57_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFG9717765.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(4482..4712)
                     /gene="dltC"
                     /locus_tag="ACGI57_002696"
     CDS             complement(4482..4712)
                     /gene="dltC"
                     /locus_tag="ACGI57_002696"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFG9717766.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(4731..5879)
                     /gene="dltB"
                     /locus_tag="ACGI57_002697"
     CDS             complement(4731..5879)
                     /gene="dltB"
                     /locus_tag="ACGI57_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFG9717767.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(5879..7393)
                     /locus_tag="ACGI57_002698"
     CDS             complement(5879..7393)
                     /locus_tag="ACGI57_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HFG9717768.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDEKDFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(7514..8704)
                     /gene="dltD"
                     /locus_tag="ACGI57_002699"
     CDS             complement(7514..8704)
                     /gene="dltD"
                     /locus_tag="ACGI57_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFG9717769.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
BASE COUNT         2807 a         1597 c          871 g         3584 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaagtcac aagtgtgcat
       61 agctattttt taatcaagag aagtctcttc tttcatagac aatattaact ctattctttc
      121 tagtatttta tctcttggaa agaaagtctc ttgacctgta aaagctgatt tttttataaa
      181 ccattcctct ggtattatgt tttttctttt ccatctatat aattgtccat aagatatatt
      241 agttttgctc aataaatctt tcttagatat taattcttcc atagctcttc tagcccacct
      301 ttatactgtt ttttacagta gaattatctg aaactgataa attatcactg tattcaatat
      361 ttccaacatc ataattatct actagagata tgcttttttt ctagcatacg aaattttttg
      421 caataatctc catgcccctt ttttatattt atactacttg ctccaacttc attaaaatta
      481 gaagttccat ttaaactaat attaacttct tcacaattca aaaatccatt acactccaag
      541 attccactta aattgatatt ttcacaatct atattctcat ttgcttttaa atatccttct
      601 ccatgaataa tatccgattt tatacttcct tctacattaa taattccact agtatcaatt
      661 tcaacagaat taatatttcc tgtacatcta agaagtcccg atatctcaac ttttgatgcc
      721 agtatgtttc cttctacttt acttaaacca gatgtaaaaa attcattaca ttctgttgtc
      781 ccaaaaactt ttgctgaacc agaagaatca aatttactac attttacact tccttctatt
      841 ttgcctgttc ctgatatatt aacattatca aaaattccac ctttacattt tccaacacca
      901 tcaaatctta gtgaacttaa ttcttcattc ataatgatat cctttcttta ccgtatttta
      961 tctttaaacc taatataaca aaacattgtt acgttgtcaa gtatgataat aatacttaat
     1021 aaagcaacta tcatttattt tctataaata ggcataaaaa taatagggaa atgctttatt
     1081 taaactataa atctaatttc ttagattgtg gtttaaataa aatatatccc tattactcaa
     1141 tgtttatata ttcattgtat aaacttctaa aatataagtc tttaaattct actgtcaata
     1201 ctttttatac actgtttgtg ctatcatctc acacacctaa aggagtaggc tttgcgcact
     1261 attctgtaac ctacaagaca ctatcttctt ctatctattg ataaattttt ctattacatc
     1321 agctagtctc tccatatcag ttggatagac ttcacctatg ctacctattc taaatgtatc
     1381 tatatcagtc aatttacctg gatagataac aaatccatta tcttttaaat aagtataaaa
     1441 ttccataaat tcaaactttg catttttagg atataagaaa gttgttatta caggtgattg
     1501 agcattatca ttcaccaaag tatcaaagcc taaagatttt aatcttgaag ctattgtaaa
     1561 ctgattttct ttatatctag catatctttt ttcaacagaa ccttcttctt ccaattctaa
     1621 taaagcttga taaaaagctc ttacaacatg tgtaggtgaa gtaaatctcc atttaccatt
     1681 atttttctcc atagtctccc attgcgcata cacatcaaga gataatgatt tagctatacc
     1741 tttacattta gataatttct ctttattagc tattataaaa ccaaatccag gtactccttg
     1801 tatacatttg ttagaactac taactaaaaa atctatatta aaatcttcaa catcaatctc
     1861 aattccccca aaactactca tagcatcaac tatataaatt ttgttatatt cttttgcaag
     1921 tttccctacc tcttgtatag gatttaatct acctgtagta gtttcacagt gaaccattga
     1981 tatatgtgtt atatccttgt tttcttttaa taaattttct actacattta aatccactgc
     2041 ttctatatct ttaaatgtgc agtcaacaaa ttgtatgtta agataattac atatatcttt
     2101 cattctcttt ccatatgctc catttgcaat tacaagaagt ttaccatctt ttgaaatagt
     2161 acttcctatt atagcttcta ctgaaaaagt cccacttcct tgcattaata cacttgtgta
     2221 tttatctgta ttttgggttg ctagagatac taatcttctt ctaacatctt gtactaggtt
     2281 attatattca acatcccaag tacaccaatc ttttaacata gatgctctta ctgatttagt
     2341 agtagatagt ggtcctggtg taagtaatat gtaatcattt tctggtacaa aatctgattt
     2401 aatagtttca ttctctattt ttattagtat atcttctaac tcagccatag tttctattac
     2461 aaaatgtgca cctgcctctt taaatttctt tgaaactata ctcatcttag cttttaattc
     2521 ttccttatcc atgttctcta cttcttcttg tgtaagccct aactcacttg aaccttttat
     2581 tacagcaaca ctccacattc ctgcatttac gccttctttt acatcactta ttgtatctcc
     2641 tacttttacc atagaactca taggagacac ccctaaagct tcagcatttt tatagcacat
     2701 ccaaggatac ggtcttcctt gagatacttc tgatggtgtt acaagaaaat cgggtgagta
     2761 tcctttcttt gctgcatttg gttcaactat gttcatcatc tctcttgtat aacccgttgt
     2821 agaacctatt tttaatccat tttttctcaa cttttcaata gtttcaacaa catgaggaat
     2881 tggagtcgta tactcttcta atgtctcaaa taacattggc tcaaattcag cataaagttc
     2941 atttacatca tcttcagttg gcactttacc aaacttatct gaccaaatat tctttattct
     3001 atccatttca cacatttctc ttatatggtc tatttttaat tttcccatag gttttcttgc
     3061 ttcttccatt gttacatcta tacctcttct tttaaatatc tctataaata cattaagagg
     3121 tgcaaaacat ccataatcta ctgttgttcc agcccaatcg aatactactg cttttatttt
     3181 ttctccatat atctttttca tatttatagc ctccatagat tctttttaga atttttgtgt
     3241 ttttaattgt ttttactttt aaagtataac tttcatatgt taattataca tcatatttag
     3301 gttaaatata tattaaattt ctgtttttag ttgtatttag ttgtttttat tttgtatcat
     3361 attcccataa aatcctaagt tacaatctaa aatttacttt ctaatcttaa tctatgaact
     3421 gactatattt tatgaattta gttgtattga gcatagttgt attatataaa actaagggtt
     3481 tattttgctt ctatccatgt tatatttatc tcatttaact tatccttcca ttcattcgga
     3541 ggttctacat cacaaataac aaaatctaat tcctctaaat ctgctatctt atacatacta
     3601 gcaattccta ttttagaact atcacataat acaaaacttt cctttgaatt ttttatatat
     3661 tcttttgaca acaatgcctt attttggtct atactactaa ttccaaaact atcaagtata
     3721 ccttcactag atataaaagc tttatctacg tataagtttt tcatagtatc tattgacatt
     3781 ggacatgctg tcctttggtg ttttaaattt acttttccac caataaatat aacttctcca
     3841 tcaaacaaac cttgattctc ataagaaatc aattttgata gaactggata agagtttgta
     3901 ataattttta aattgtttac agatattata taatctacaa tctgcaaatt ggtagtacct
     3961 tcatctatga taattgactc attgtcattt acaaacttag ctgcatattt agcaatactt
     4021 tgctttttat ctaggttagt attgtttctc tttatatgtt ctggttcaat cactccataa
     4081 tttattttta tagcaccacc atataccttt tttaatttat tttcattttc taattcttcc
     4141 aaatatcttc ttattgtttc tgatgatact tctaatcttt ttactaattc tttagtgttt
     4201 accttccctt ttgaatctaa taatttaagt atttcttttt ttctatctat cgaatttaaa
     4261 gacattcatt ttcctcctaa atataatata tttttctcct ataatattac aatgatacct
     4321 aagatttatg aaatcatatt tatgtattaa attttttact taattaaagt tattttcaaa
     4381 agactttgta ttatcattaa aaaagtccat caaatattag tatcagatgg actttgtaaa
     4441 attttatttt ataaattaat atttaaagtc taactactaa tttattttct tgtctccaag
     4501 aattttacta aattatttac agtagacata tcttttctct ctaaatcagt tggttgaagc
     4561 tctatcccta atctattttc aatttcaagt aaaacttcaa ttatagctaa tgagtctaaa
     4621 agttccgtct caaataaatt aaggtctaaa tcttctctta tctcatcagt ccctaataca
     4681 tcttcaaata tttctataac tgtttcttgc ataaattttc ctccttaaaa ttaagctaaa
     4741 taacctgaga atatcagcaa tccaaaacac acaatatgga atgtaatgaa aatctgcact
     4801 ctctcgaacc atttatcttt tttatgtttt ttataaaatt tagattttcg ctgatatata
     4861 tcagttaaca caagtgccaa tccttggtat acaccataag caacataata ccaagttaga
     4921 ccatgccaaa atcccattgt tatcatggtt atcatctgag ccacatgagc agcagtagtc
     4981 cttttcttaa atcttttctt tctcattgat gacataacaa atctagaaaa aatataatct
     5041 ccaaaccatc ttgaaagact tatatgccat cttgtccaga actctttcat atctttactg
     5101 ataaatggct tgtcaaagtt tataggtact tgtattccaa atatgtatcc tgtacctata
     5161 gcaaataaac tatatcctgc aaaatcaaag aacaggtata aactatatgc gtacatataa
     5221 cttaaaatat ttataaaact catatcttta ggtattcttg atacccaata tgtatttatc
     5281 aaaaatgcta taacaaactt ataacctact cccatgacaa tattttttat tcctggcagt
     5341 aagtattctt ctatatactc ttttctagag atttgttttt ccaagtcctg ttcaaatctt
     5401 cttgacctat caataggtcc agaacttaaa gttggaaaaa ataacataaa ataaagcatc
     5461 tttgaaattt taacttcttt tatagctcca tcataaattt caataaccat ttgtatagtt
     5521 ctgaaattta agtatgatat tccaataaat ccaattattc caaaagaagt aactggagat
     5581 attttattta ttattattgg gagcattgat gcaaacaaga atccccaata tatgtattta
     5641 tttttagttt ttcttcttac atattcatat cctttaacta ttattacttc aagaataata
     5701 aatattacca agtactttag ttggacatca attcctacta ttaagaatat cataaacaaa
     5761 cttgccaaca taccataata ctttatattc ttgcccatta gacctaaaat tactgctggt
     5821 attgatgtta ataataatat ataaagataa aaataatctc catattgtga aaaaatcatt
     5881 aaatttcctc cataagtttc tttctgtcta ttttaccatt gatatttgtt ggaaactcag
     5941 agattattga aatatttctt ggaatcatat atgaaggtat atactttcca agttcctttt
     6001 ttattatcat cccattttta atattactta aatcattctt ttcatttaac tcaacaatac
     6061 cttttaagta agcaattttt tcgtccttat atactggtaa aactactgca ttttttacat
     6121 tatgtacttt tctcaaatta ttttctatat cttctatttc aattctaaat ccatttaatt
     6181 taatctgaaa atcttttctt ccacaataat atatatttcc atctaaaaga tatcccatat
     6241 ctccagtttt ataagctctc cactttacac catctatttc atcatagaaa aatacttcat
     6301 cagtcttttc tttattattg aagtaacctt tagaaactga tggtccaatt attataattt
     6361 cacccttttc attttcttta agctcatttc catcttcatc aagtattttt attttacaat
     6421 ttgacatagg gtatccaact ggaagacttt tttcatcatc tatagccttt tgagtcatat
     6481 catttacact tacaccaaca gtagcctcgg ttggtccata tccattgata attcttgtgt
     6541 ttggaaatct actcataagt tctttagtaa gattttttga aagtgcttct cctatgaata
     6601 tcattgactc aaggttagga agcattttac tattaaactc tttttcagtt atacatactc
     6661 ctgcaaaaga aggagttgac acccatacag ctatatctga gatactaaat tgtctaaata
     6721 gttctttata gtctgctaat acatcttttg atattgaaaa taatgtagct ccatggataa
     6781 gaccagggta tattgttgta actgaaaggt caaaagaata tgctggttgg ttcattatta
     6841 ctttttcact tccatctatg tttagatatg ggcttatcca atctgaaaag ctatctaaat
     6901 tgtttgaact tatttgaacc ccttttggct ttcctgtact tcctgaagta aatagtatat
     6961 atgcattttc atcatctttt acccagtttt ctttatctaa tgattttccc tgatattcat
     7021 ttattatata atttaattta tccatatcaa ttacattgat atctccaaaa tctttttcat
     7081 cactaaagtt aaataatacc tttggtttta tctctttagt gacttcaaaa actctatcaa
     7141 ttggaaaact aatatcaaga ggcacatacg ctctacctga ttttaaagct cctatcatac
     7201 atgccattat catgttttct ttatttccat aaattactat aggagtatct tcttctttgt
     7261 atacatcctt tagaaatact gatatagcat ctgaatattc atttaaatct ttataagaaa
     7321 gtttatctcc attacacatt agtgctgttc tatctgtatt tgaatatttt tttattcctt
     7381 caataatttt catataagaa tgctccttta aaatccgtta taaataaatg gcaattcttt
     7441 aaatggagta aatgttagaa ctcctatcgc caatattata accatagtta aacaaatact
     7501 agttaacaaa tctttattgt tctttaaata ttttaaataa tctctcacag acatctaccc
     7561 atcctttcgt accaagatgc attacatccc ttaaataata cttatctgac cctttatctt
     7621 taaggtttag aacttcaaag cctttgcttt ctgctatttt ttgtgcttta tcgtagtact
     7681 gatttctttc tttttcagaa ataccagtca aattatagaa tttatccata cttgggatta
     7741 atacaacaac tggcttaata cctaagtcag tacatacatt tagcatcagt ttataatttt
     7801 caaactcctt agatgttaat aaatttacat ctttgtctct tcctttgtat tgctctatac
     7861 catctttaaa atgctcttta taatagtact tatcaatatt tagtggattt ttaccaactc
     7921 tttttttagc atcttcaatg gctttctttc tctcatatga ccaatttatt ggtctttttc
     7981 tttttgttgc tctcttttta tctagcttaa ttaatcgttt ataaagaata cccttttctt
     8041 taagagctat acaatacttt cttccttgaa aataaggctc taaaagcacc ttttcagctt
     8101 tttcaggaaa tgtcttaggc tcatatagct tagcatataa tgcttctgct ttatactcgt
     8161 cacttcccca taataactta cttgattttt ttgcatattc aatcttattt tgtttagata
     8221 tttttggatt atctaaaaat cgataaaatt gtattggtga aaatctagac tgataatggt
     8281 gacttgtgac tccgtctttt tccataaacc attgcataga aattaacaag acaactttct
     8341 tattatcaat attaggattc atacttccta atattgcagc atcttgtaat gtctgagtat
     8401 aggctctccc aattgcaaac accttatttt tactcctatt tgtattaaaa tagtactttg
     8461 gatgttgttt tgttgaatta ctaagttctg atgaccccaa aatcataata tccttttctc
     8521 ttaaaaaatg attatttgct gtaacccctt tatcttttat atcacttaaa gtatcatcca
     8581 taattggaag tagatttttc tccctcaaaa gttcatcagt tttagaatca agaaatttgt
     8641 ctaacccaaa taaaaatact acgcctatta taaaaggtgt aataaagtat atcaattttc
     8701 tcatattttc tctcctctaa aaatattcaa atttatgtta ttattaatca tattagtttt
     8761 gatatgtata ttcaactaca aaattgtaat aaaatcgtca aaactttttt tgatactgtt
     8821 acaaaattgt aaaaaaaaag aaaaaaacgc caaaaataa
//
LOCUS       DAYAOX010000149         8770 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.150, whole genome shotgun sequence.
ACCESSION   DAYAOX010000149 DAYAOX010000000
VERSION     DAYAOX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.150"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            241..2100
                     /locus_tag="ACGI57_002700"
     CDS             241..2100
                     /locus_tag="ACGI57_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFG9717770.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2392..2991)
                     /locus_tag="ACGI57_002701"
     CDS             complement(2392..2991)
                     /locus_tag="ACGI57_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9717771.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3094..3891)
                     /locus_tag="ACGI57_002702"
     CDS             complement(3094..3891)
                     /locus_tag="ACGI57_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9717772.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(4004..4109)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4201..5187)
                     /locus_tag="ACGI57_002703"
     CDS             complement(4201..5187)
                     /locus_tag="ACGI57_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFG9717773.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     regulatory      5351..5527
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            5671..7401
                     /locus_tag="ACGI57_002704"
     CDS             5671..7401
                     /locus_tag="ACGI57_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFG9717774.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLSIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7511..7591
                     /locus_tag="ACGI57_002705"
     tRNA            7511..7591
                     /locus_tag="ACGI57_002705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7545..7547,aa:Leu,seq:tag)
     gene            7926..8441
                     /locus_tag="ACGI57_002706"
     CDS             7926..8441
                     /locus_tag="ACGI57_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HFG9717775.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
BASE COUNT         3169 a         1075 c         1334 g         3192 t
ORIGIN      
        1 taggtggttt gtaattaaaa agtttataaa ttatattaat agtaataatc tataagaact
       61 tatatattag aaaaaaattt ttgtatatat aaaggactct aaagatgaac tatgtaaaac
      121 aaggtttata tattctaatt gtatagaaaa atttattgta aacgcatgta tattattaga
      181 aaaaatagtt tggaggtatt caataaacat aaaaaacaat ataggggata aaggtgtttt
      241 atgaagagat ttttgaggag aattatctta gttttattta ttttgttgat ttttataagt
      301 tttatttcta ttaaattaat tcataatgtt ggagattatg gaaaattgat taattatgtt
      361 gggattgttc gtggagctag ccaacgtcta actaaactag agatgaatca tcaaccaaat
      421 gatgaattaa tagaatatat tgatgaaatc ctacaggagt tgattacagg gcatggagat
      481 tatggtttag taatcactga ttgtaatgaa tacaatgagg acttactttt attagaaaaa
      541 aagtgggaag atttaaatat tgaaattaaa aaagtaagaa tgaaagaaca gaataaccag
      601 ttattaagta taagtgaaga gtttttttct cttgcaaatg atacagtgtt taaaatagaa
      661 aacttttcta aagaaaaaag taattactta atgactttaa taataattat atctattata
      721 ggtatattag catgtatcat attgatttta caatatagta aaaaaatggt tagactggaa
      781 aaattaaatg tagatttaaa aaatattgca tataaagatg aactaacagg agtaaatact
      841 atagaaaaat ttaaattgga tgcaaatcaa aatatttgta tacataagga taagaaattt
      901 gctgtattct atattgattt tgaaaacttt aaatacatta atgatatatt tggatatgat
      961 tatggtgaca tgatactgaa aagatatgct aatcttatga tgaatgacat agggaaatat
     1021 gaaatatttg caagagaaat tgcagataga tttgttgcgt taagatgcta tgtagataag
     1081 gaagatttag tggtaagaca acgagatgta gacaatgaac taattaatat aacaaatgaa
     1141 attaaaaata aacatagtat tactgttgtt agtggaattt gttgtattga agatgtaaat
     1201 gaaaaattaa gtatagatgg tttaataaat agagcaaatt ttgcacaaaa aactgttaag
     1261 aataaaccag gtactaatta tgctttttat aatgacagta ttcgtaaaaa aatgattgaa
     1321 gaaaatacta tcaaaagtag aatacatgaa gctattgaaa aaagagaatt tatagtttat
     1381 ttacagccta aagttaattt acatagtcaa aaaataaatt gtgcagaagc tttagttcgt
     1441 tggcttactc cggataaagg tattatatct ccagcaatat ttattccagt acttgagaag
     1501 aattttttta ttgccttagt agataagtat gtttttgagg aggtttgtaa atggattaga
     1561 aagaggttag atgaaaataa accttttgtt cagatatcag ttaatgtttc aagaatacaa
     1621 ttttataaca ctaaatttgt agaaacttat tccaatatac aaaataaata caggatacct
     1681 aaaaatacta tagaaattga atttacagag agtgttgctt ttgagaatca aaaacatctt
     1741 ttggaaatta tacatgattt gcatgaaaat ggatttactt gttctttaga tgattttgga
     1801 aaaggttatt cgtctttaag tgttttaaaa gatttaccat ttgatgtttt aaagcttgat
     1861 tctatgtttt tcaaggctag tttagataaa gataaagaga aaatagtaat aaaaaatatt
     1921 gtacatatgt taaaagaact taatatcaca actgttgctg agggcattga atgcaaggaa
     1981 caagttgaat ttcttaggga tattgggtgt gatttagttc agggatttgt attttataaa
     2041 ccaatgccaa tattagaatt tgaaaaaata ttggataaag agtttgttta taatagctaa
     2101 aatgtacttt ataaaatatg aactaattaa gtaaaattat tttgtactga ataaattttt
     2161 actttattta cagaataatg attatttaca gaaaagcgcg aaaagtgtat tcctttaagt
     2221 gtgggatgga tagcacaaat agaggagaaa aaatattggc catataagaa tgcataataa
     2281 tataaatatt taaaaggtca gcattatata aaaattaaaa agaaaataaa taatttttct
     2341 ataaaaaaga gactatgaga aaatttactc atagtctcct aaataatgta tttattttct
     2401 tcctgcaacc cattttaatc cccaatgaaa ttcagtatcc atttctctat gacctttgaa
     2461 gtatttaact actttttcaa cactgaatgt ctcatcattt acattttcta tcaatgctat
     2521 tgaacacata ttatagccat ttttatgttc atcaagtttg acaactaagt ctgaatcttg
     2581 tttttgtttg attgttacaa ttccatcagc ttcagaccag ttagctacac cttcgtatat
     2641 aaaagcataa ataagtattc tttttatatc ttttatttta ttaccattta ttcttaaatt
     2701 ttctccttgt gtattacttc ctgttctgtc atctccatct aattgagcaa aaggtggatt
     2761 gtctaaacta ccaaaggcat ttcctaatgc ttgaacagca ccttttacac catttttcat
     2821 ctcaaatagg caacccaaat ctaaatctat attactgctt cttaaagagc caaaaaaacc
     2881 ctttttttgt actttttgat tccaatttaa attaactaaa atttcaccta aatcgctatt
     2941 ttcctttttt gtaaggttta ttttttgtcc cttttttaat tctatagcca tagtaatctt
     3001 cctccacaaa catagattta tttaaatggt agcacttaat aagtcaatat tcaacaagat
     3061 tagaataata atattaaaat tatactaaat atactaaaaa gtaggaatca caataccttt
     3121 gtatttttct tcaattaagc ttcttgcttt atctgatgta agaacatctt gtagaacttt
     3181 aatcttttca ctgtctttat cttcagttct aaatgctaaa agatttgcat actcgtcatt
     3241 tataggagct gtataaattc cgtttttctt ggcatcaaga ccagcaggta cagcataagt
     3301 agtatctata aatgcagcag tcacatctgg aagagaagat ggaatttgag caatatctaa
     3361 ctctttgaat tgtattccct ttggattttc tattatgtcc ttaggactaa ctaatccatc
     3421 ccctttaggt aacgttaaaa gtccaatact ttcaaggtat ctaaggttct tagaacaaga
     3481 agatgggtta ttgcttattg caattgtatc tcctttttta aattcatcaa cagattttat
     3541 cttataagag taaagtattg ctgggcatac atatagtttt ttaccagcca ttattttgta
     3601 acctttggcc ttaacagctt cttttagata aggttcatgt tgaaatagat ttgcatcaat
     3661 ttcaccatta tttaaagctt cgtttggaag tatgtaatca tcaaagtttt taacttctaa
     3721 agtatatcct ttttctttaa taagtggttt taattcttct aataactctc ctcctggaac
     3781 taaagtagca ccaacaacaa tttttttatc atctttagct ttagaacatc caaccacagc
     3841 taaagttagt actagacata ataacaaaca taataatttt ttgaatttca tataaattcc
     3901 ctcctgaata taattgatta gttaaaaata aaataccatt ctttatcaca ctgcaaaata
     3961 aaaataagcc tgagtttaaa ctcaggcttt aaaagctaat ttagactcat ctttcagtat
     4021 aatactgcag gatttagcac caatgtatat aaaaatatac tggttgctgg gtttcatagg
     4081 gccagtccct tcaccactct ggataagaag tatcaatttt taattatgtt ataaatatta
     4141 tcatgatttt ttacgtctgt aaacataatt ttataaatat tttaaaatct ttaatgtatt
     4201 ttatttaaca cctaaaattc cagaaactag aatatcagta tcagcaccta agaaatagtc
     4261 atttatttca atagaatcca gagtatttct caaagcattt tcttcatgcc ttataccaat
     4321 aagtttattt tctacaaatg caacatcaca ttttccaaaa aaatctccaa aaaattttat
     4381 actagttatt atgcctttat caacatttaa attaaactca acatttccac cagaatattt
     4441 taattcattt gaaagagtat attttggtga attaccaaaa ttccaatccc aagtttcgta
     4501 ttttgattta gtaagctttt ctatttgttt tatatcatgc tcactcaaac aataattagt
     4561 attatcagta tttgttttag aaagataatc cattatagca tctttaaatt ctaatatgtc
     4621 catttttgaa tgtaaatgtt cacttatatt ggttacacga gattttacag attttattcc
     4681 ttttccttca aattttatag gtttaacctt taatgctttt gagaggtcat ttatttcaga
     4741 tgaaaataaa agagtcccat gatgcattac tttgtttttg tagttgtatt gagcatttcc
     4801 agaaaatttc ttgccatcta taagaaggtc atttcttcca gaaaattcgg cattgacatc
     4861 tagtgtttta agtaaatcta ttatgggctg agtgaatctt ctgaaatcac taaaattatt
     4921 attgttacaa gctataaatg taaaatttat atttcccaaa tcatgaaata cagctccacc
     4981 accagactgt ctcctaacga caggtataga atattctttt atatattcta aatttatttc
     5041 agaaagtgtg ttttggtttt ttccaacaat tatagaaggt tcattcctcc ataaaataaa
     5101 taagtcttca tcaaaatttt ttagaagata ttcttccatt gctaaattaa aataagggtt
     5161 tgttttttca ttataaatta ataacattaa agtagttcct ccatagaatt gataaaattt
     5221 ttattatgaa tacaattaat tttataattc gacaataata taaaaactcc tttaaattag
     5281 aaattttaaa aactaccttg caaagtttaa ataaataata tatactcaaa atatgtttaa
     5341 taaaagtgaa tagaagggta gaggcgcaat aatctatcag tactaataag gagttaagca
     5401 cagagagtta ttaggaaagg agatattgcc gaagttttat aatcgatgct ttaagattat
     5461 attgctgggg gtacatagaa aatatgtaca actgtcacag gtttatttgt ggtgagctat
     5521 catttaagga attatattat atttattttt actattgttg atatattgta atgtttaagc
     5581 cttgagtgtt taccactcaa ggctttttta gttttattgt aacataatac cctgtacagt
     5641 tcacaaaatt aaaaggagag ggagatttat atgaggaaca aaaaaattaa tattattttt
     5701 ctgacgacaa tcatgttcat aatgtcaaca gtaatggttt ttgcagaaga agatatagac
     5761 actattgcac tagccaatgc agaaaaattt gggattttaa ctttaatacc accattagtt
     5821 gctataatac tcgcatttat aactaaaaac gtaataattt ctttacttat aggtattctt
     5881 tctggtagtt ttataattaa agctagtggt attaatgttt ttgcgacatt tatacaggca
     5941 tttttagatt tagtagatag agctttagta tctttagcag acccttggaa tgcggggata
     6001 atactacagg ttttgtcaat tggaggagta ataaatcttg ttgctaaaat gggaggagct
     6061 aaagctatag ctgaagcact tgctaaaaga gcaaagtcag caaaagggac acagcttata
     6121 acatggtttt tggggctttt agtattcttt gatgattatg ccaattcact aatagtaggt
     6181 cctatgatga gacctgttgc agataaaatg aaaatatcga gagaaaagct agcatttata
     6241 atagatgcca cagcagcacc agttgctggt ttagctataa tatctacatg gataggtctt
     6301 gaggttggtt taatacatga tgcttttgaa agtataagta tagatgtaga tgcatttggt
     6361 atatttttaa atacaatacc atttagattt tacaatatat taatattagc tttcattgta
     6421 atatcagcat tattattaaa agaatttggt ccaatgagaa aagctgagat aaaatctaga
     6481 agtagaaaaa ttagtataga ccttgatgaa ggcgtagaag agttggatga tttagctcca
     6541 aaaaatggag taaaattgag tgtatggaat gctataatcc caataggaac attgataata
     6601 gttgcattag catcatttta ttatagtgga tatacatcta taatgggagg agacgataag
     6661 gctctgatac aactatttac aaattctcca tattcttttg aagcaataaa agaagcattt
     6721 agtgcatctg atgcatctag agcattattc cagtctgcac ttgttgcaag tttagttgct
     6781 attataatgg ctgtagtaaa aaagattttt acaatatcag aagcaattga tgtttggata
     6841 gatggaatga aaagtttagt aatcactggt gtaatattaa tacttgcatg gtcattaagt
     6901 tcagtaataa aagagttagg tacagctaaa tttttaatac acctactatc aggttcacta
     6961 ccaccattct tattaccaag tttaatattt ggtttaggag caataatatc ttttgctact
     7021 ggaacagctt atggaacaat gggtatactt atgccacttg caattccatt agcatactca
     7081 ttaaatcctg atatgtctta tgtaatagta agtactagtg cagttttaac aggtgcaata
     7141 tttggagacc attgttctcc aatttcagat acaacaatac tttcttcaat gggagctggc
     7201 tgtaatcata tagaccatgt taacactcaa atgccatatg ctatatttac agcagtaatc
     7261 actattgtat ttgggtatat accagctgga cttggattgc caatatatat agtattacca
     7321 gtggcaatag ctgctatatt tgtaggtatt caaataattg gtaagaaagt tgatgaagcg
     7381 gaaatagaac ttgtagaata aagatataaa taagtttcac cttttattat cactattttg
     7441 ttagttttta gataatttta aaaagttatt gaaaataatg gcgaaatata gtaaaataaa
     7501 agagtgataa gcggatgtgg cggaattggc agacgcacta gacttaggat ctagcgccat
     7561 tggcgtgggg gttcgactcc cttcatccgc actttacagt acttaattgt actgaaaaaa
     7621 actggggttg ctggctttcg gagtgggcta tcccgttttt atttttttgt atatataata
     7681 gattggcact ttagtataag gtgctttttt tataggcaaa aacagtacat ttatttctgt
     7741 tttattatca ttcatctatt agaatatact ttagttaaga atgtgttaat aagcagtgtt
     7801 cacattcttg ataaaatgtg gaataatatg tatttttact aagacggaaa tgaaataatt
     7861 aaaccattaa ataatattat aagatgatga ataagtagat aaatcaaatt ttgtaggagg
     7921 atattatgga aaaatatata tggttgtttc cattactttt tatttttcat gatatggaag
     7981 agataatagg ttttggaata tggcttaaaa aaaataaatc aatgcttgat aagaagtatc
     8041 catttatcag taaagtttat gaaaattata gtactgaagg catggcattt gctgtgtttg
     8101 aagaatttat tttatgcatt attttctgta tacttacagt tataacagag aatcagtatg
     8161 tatatctact atggttaggt agttttatag catatacctt acatttggta attcatatag
     8221 ggcagtcaat cataataaga aaatatatac cttcacttat taccagtatc atatgcttac
     8281 caataagcat ttggtgtata agtaagagta tttatatagt tgattgtgaa atgagtacaa
     8341 caattctgta cagtataatt ggcattataa ttgtagcatt gaatttaaag tttgcacagt
     8401 cgctgattgg aaaatttaca aagtggatgt caaacatata aattttagtt tattggacag
     8461 ttcaaggaat cagaaattgg aattttatag tattaatatt ttatggtatt aatataatgt
     8521 ataaaatttt atagcaatgt atttcataag tataaagtga ctagaatttt aagaaatata
     8581 caaattctat aaaaactaaa taatttcaag tatattttaa aaatcttatt tttcacaagt
     8641 ttaaaattgt aaacgatttc atcaagtcta tgaatatact tttaacatgt tatagtaaat
     8701 ctcaatataa atttacattt ggttctttgt gtgttatttt aataataaat taagtgaaaa
     8761 tgttatttta
//
LOCUS       DAYAOX010000150         8750 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.151, whole genome shotgun sequence.
ACCESSION   DAYAOX010000150 DAYAOX010000000
VERSION     DAYAOX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.151"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..831
                     /locus_tag="ACGI57_002707"
     CDS             <1..831
                     /locus_tag="ACGI57_002707"
                     /inference="COORDINATES: protein motif:HMM:NF025626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HFG9717776.1"
                     /translation="IKIKIYRIYLKIIMQLEKEISMQIIDININNIDNEHICCSISEK
                     KGENCVFSKKAWMKNNFDKGLVFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFW
                     ISGKFKGQGYSNLLLQECVDDAKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADT
                     SEPNYELLYFPFTDNAPIPYFKNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIAN
                     SRGIDVNLIKFETAKEAQNAPAPFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN
                     "
     misc_binding    1049..1262
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1516..2550
                     /gene="argC"
                     /locus_tag="ACGI57_002708"
     CDS             1516..2550
                     /gene="argC"
                     /locus_tag="ACGI57_002708"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFG9717777.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2591..3808
                     /gene="argJ"
                     /locus_tag="ACGI57_002709"
     CDS             2591..3808
                     /gene="argJ"
                     /locus_tag="ACGI57_002709"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFG9717778.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3823..4683
                     /gene="argB"
                     /locus_tag="ACGI57_002710"
     CDS             3823..4683
                     /gene="argB"
                     /locus_tag="ACGI57_002710"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFG9717779.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKETE"
     gene            4713..5900
                     /locus_tag="ACGI57_002711"
     CDS             4713..5900
                     /locus_tag="ACGI57_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFG9717780.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVILKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6009..7031
                     /gene="argF"
                     /locus_tag="ACGI57_002712"
     CDS             6009..7031
                     /gene="argF"
                     /locus_tag="ACGI57_002712"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFG9717781.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7158..7775
                     /locus_tag="ACGI57_002713"
     CDS             7158..7775
                     /locus_tag="ACGI57_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HFG9717782.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(7995..8693)
                     /locus_tag="ACGI57_002714"
     CDS             complement(7995..8693)
                     /locus_tag="ACGI57_002714"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFG9717783.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT         3520 a          854 c         1575 g         2801 t
ORIGIN      
        1 attaagataa aaatatatag aatatattta aaaattatta tgcaattaga aaaggagata
       61 agtatgcaga ttattgatat aaatataaat aatattgaca atgaacatat atgttgttca
      121 atttctgaaa aaaaaggaga aaattgtgtt ttttctaaaa aagcttggat gaaaaataat
      181 tttgataaag gtctagtctt taaacgattt gatgtaagag gtaaagcttt tatagaatat
      241 attccagcag aaaatgcatg gtgtcctatt actgcggaca attatatatt tattaactgt
      301 ttttggatat ctggaaaatt taagggtcaa ggatattcta atctattgct acaagagtgt
      361 gttgatgatg caaaatcaaa aggtaaatat ggattagttg tgctttcgtc tagtaaaaaa
      421 atgccttttt tatctgatgc taaatatcta aaatataagg acttttgttt agcagataca
      481 tcagaaccaa attatgagct attatatttt ccttttactg ataatgctcc tattccatat
      541 tttaagaatt gtgtaaaaaa aagaaaatta aatgagaacg gtatagtttt atattacagt
      601 aatcagtgtc cacatacgga aaaatatgct cctattttga aaaaaattgc aaattctaga
      661 ggtattgatg taaatcttat aaaattcgaa acagcaaaag aagcacaaaa tgctcctgca
      721 ccatttacta catacagtct tttttttaat ggtgaatttg taaccaacga gatactttct
      781 gagaaaaaga ttgagaaatt tttagatacc aacattaaaa aatttaacta aaaagagatg
      841 taaatattgt attttgaaaa tatattattt tataatttaa tatattttgg atgtttttta
      901 aattgacgaa tgaaatataa aaattatatc gcaatttaga tacaaaatgc tggaaattaa
      961 ccatatagaa aatatttcca aaaatttaaa aattatattg acaaagttaa aaaaataaac
     1021 tatcatataa aacaataaga aaataaattg aaatcctgtg aaaaagagag tagcataaag
     1081 ctagttgata gagagctggg aatggtggaa gcctagtaac gaattttatt gtgaatagag
     1141 ctttggagga gattaccaga agtaatagta tggtaattgg tgaatctcgc cttaagagat
     1201 tagagtgggt aagattatct tatcaattag agtggtaacg cgggtgtata ttcgtctctt
     1261 ggtctttgac caagaggctt ttttaatttt aaatgtacat tttgtagata ctttttaatt
     1321 tgagttttaa tgattattgt aattttacca ataagagtgg taccgcggaa aatcgcctct
     1381 tagtatttat aatgctaagg ggttttttgt tttaattaga gtattttaat ctacgtatta
     1441 tcaaatgatt atcatatagt aaagtggaaa gataggattt taagactatt tgaatataaa
     1501 aatggaggga acaaaatgaa taaaataaaa gttggtatta ttggtgcaac tggatatgtc
     1561 ggggcagagt tgattagatt attaatgaat catgataaag tcgaagtgac agcgataggt
     1621 tcaaattcgt atgttggaaa agatatagtt gatatatatc caagtatagg atataaaaat
     1681 aacatgatat gtatagaaaa tgaaaaagtt atagatatgt gtgatgttgt atttacagcc
     1741 cttccacatg gagttagtga aaagtttgta ataaaggcaa ttaaatcgaa aaagaaagtt
     1801 atagatttag gtgctgactt tagaataaaa gatgaagaag tatatagtaa atggtatgga
     1861 gtatctttta tagataaaat cttacataaa aaagctgtat atggattaag tgaaatatac
     1921 aaagaagata taaaagatgc agatataatt gcaaatcctg gatgttatcc aactagtatt
     1981 tctttaccat tgatgccatt actaagttct aaattgataa aaaataataa tataataatt
     2041 gattctaaat caggtcttac aggtgctggt agagaattat cgattagtag tcactttacg
     2101 gaggttaatg aaaatatcac tgcatataaa atagggaagc acagacatac tccagaaata
     2161 gaacaaaatt taagtgaatc ctgcaaagaa aaagtaagtg tagtttttac accaaactta
     2221 atcccagtaa ataggggaat cttatcaact atatattgta caaaagaaga taatatttct
     2281 ataaatgata tacataggaa attaactgat tattatgaat ttaaagaatt tatagaaatt
     2341 cttccattag ataaggtagc ttctttaaag aatgttagat tctcaaataa atgtgtaata
     2401 tcattacatg aaaatggaga tacattgata atctgttcag ctatagataa tatgattaag
     2461 ggagcagcag gtcaagctat acaaaacatg aatataatat ttggtattga agaaaataca
     2521 ggtttaaaaa atattgcacc atcattttag aaataaaaaa tactaaactc atataattag
     2581 gaggataaat atggagatta taaagggtgg agttacagtt tcagaaggat tttttgcttc
     2641 agggattcac tgtggcttga gaaaaaataa agaaaaaaga gatttagctt tggtttattc
     2701 agatgtttta tgtgatgcag cagcagtagt aactcaaaat aaagtaaagg gcaatcctgt
     2761 ttatgttaca caggaacaca ttaaaaatgg aaaagcacaa gctatcatag taaatagtgc
     2821 taatgctaat acctttaatg gaaaagaagg acttataaat gcttataaaa tggctaaatt
     2881 caccagtgat aaattaaata ttaaagaaag tgatgttttg gttgcatcta caggtgttat
     2941 aggaaagcca ttaaatatag atttaataga agaaaatatg gatgaattag tgaataatct
     3001 aagtaaacaa ggtcatattg gagcaagaga agctattatg acaactgata ttataaaaaa
     3061 agaaatagca gtagcaataa tgtatggaga taaaaaaata actattggag gtatggcaaa
     3121 aggttctggc atgatacatc ctaatatggc aacaacgtta ggatttataa ctacagatgc
     3181 aaatattgat ggagttttgc taaaagaagc tttaaaaatt gctgttgaca aaagttttaa
     3241 tagagttagt gtagatggag atacaagtac taatgatatg gtaatgattc tagcaaatgg
     3301 aaaagcaaaa aatgatagaa taaacaaaaa agatgaacat tatcaagtat ttttgagtgc
     3361 acttacatat gtctgtatag aacttgctaa acttgttgca aaagatggag aaggatgtac
     3421 aaaactaatt gaatgctata taaatggagc attaagtgaa gaacatgcag taaaattagc
     3481 aaaaacagtt atatcttctt cattagtaaa gacagctgta tttggagctg acgcaaattg
     3541 gggtagaata ctgtgtgctt taggttatgc aggtgaagaa atagatatgg aaaaagttga
     3601 tattattttt gagagtatga aaggatatat tgaagtatgt aaaaatggaa atggattaga
     3661 ttttaatgag gaaaaagcaa aaaaaatcct tgaagatgac gaaataagta tattagtaga
     3721 tttaaatatg ggcaatgcca gaggaaatgc atggggatgt gatttaagct atgattatgt
     3781 aagaataaat ggaagttata gaaattagga ggatatactt taatgattaa tatagaaaaa
     3841 gcaaatacac ttatagaagc tttaccatat atagaaaaac atcagggaaa gactattgta
     3901 gttaagtatg gtggaagtgc aatgaaaaaa gatggtctaa aagaatctgt tatggaagac
     3961 cttgtgttaa tgagttatgt tggaatcaat atagttttag tacatggtgg aggggcagaa
     4021 atcaataaga tgctagcaaa agtggatata gaaagtaaat ttgtaaatgg tcttagatat
     4081 acagatgaag agaccatgga aattgtaaaa atggtacttg ctggaaaggt taataaagac
     4141 ttagtaaata aaatacatac aaaaggtgga aaagcagtag ggctttgtgg aatagataat
     4201 aatatgattt tatgtgaccc atataaaaat tatgaacttg gatttgtagg agaaatcaaa
     4261 aaagtaaatg ttgagcttat agaatcttgt ctaaagtcag gttatatttc agtaattgca
     4321 actataggtg ttggagatga tggagagact tataatataa atggtgacac agcagcttct
     4381 gctatagcta aagagttaaa tgcagataaa ttgatacttc ttacagatgt ccctggtttg
     4441 ttaagagagc cagatgaaga aaaatcatta ataacagaag ttatattaga ggatgttgat
     4501 aaattatttg aagaaggtat aattactgga ggtatgatac ctaaaataga gggatgcgta
     4561 gatgccttaa ataacggagt aaatagggta catattttgg atggaagagt tccacattct
     4621 ataataacag agctatttac tgatagtgga ataggtacat taataagaaa agaaactgaa
     4681 taaaattata agtttaagag gggagaataa gaatgaataa cgacaataca atttcaaaat
     4741 ggaatgaata ttttatagat acatataacc aacccaattt tgtcatagat tatggtgaag
     4801 gaagttgttt ttttgatact aatggcaata aatatataga ttttacaagt ggatatggag
     4861 tatcttctct tgggtatagt aatagtaatt taaagaatgc tctaaaagag caagtagata
     4921 aattattaca tacttcaaat ttatatttta atgagccagt gttgtattca ggagagaaaa
     4981 taattaattc aagtggaatg gctaaagtat atttttgtaa ttctggaaca gaagcaaatg
     5041 aaacagcatt caaaatagca agaaagtata gtagtgacaa atatggtaat ggtagaggaa
     5101 ctatcataag tttaaaagac tcttttcatg gtagaacaat gatgtcttta atggcaactg
     5161 gtatggacaa ataccataag tatttttatc ctcttcccga aggttttaaa tatatagaaa
     5221 gaaataatat tgaagattta aaaaataatc ttgattccac agtttgtgca ataattcttg
     5281 aagcgattca aggcgaaggt ggagttaatg ttcttgaaaa agattatgtt ttggaaatag
     5341 taaaaatatg tcaagaaaag gatattgtag tgatttttga tgaagtccaa tgcggtatag
     5401 gaagaactgg taaacttttt ggctatgaat attttgatgt aaaaccagat atagttaccg
     5461 tagcaaaagg gttgggagca ggaataccag taggaggagt attggtaaat aaaaaacttt
     5521 caaaagtact aggaaaagga gaccaaggta caacttttgg aggtaattta ttagctatgg
     5581 tggcagcttc agttgtatta gatgagatat ctaaagatgg cttttataat gaagtattag
     5641 aaaaaggaaa ttatataaga aaatctattg aaagttttaa taacaaagtt atattaaaga
     5701 caaaaggtat tggtctaatg ataggaattg aaactaatat tgaatctagt attatagaag
     5761 agaaagctag aaaaaaagga ttgcttattt tgactgctgg aaaaaatgta ttgagatttt
     5821 taccaccatt aacaatcagt tataaggaaa tagatgaggc tcttgaaatc ctaaaagata
     5881 tattactaga aattaactaa atattcaaaa aatagttgat taaagataaa aattagaagt
     5941 aaataaaaaa gtaaagtgct aacaagttta attgaattta aaaagtttat aagaaggggc
     6001 gaattaatat gaataatata agtttaaaag gtaagagttt tttgacatta aaggatttta
     6061 caaaagaaga gattagatat ttattaactt tatcaagtta tttaaaaact aagaaaaaag
     6121 ttggtataaa aggcgatttg ctacaaggaa aaaatatagc tttattattt gagaaaacat
     6181 caacaagaac tagatgttca tttgaagtag cagctcatga tgaaggagca catgtaactt
     6241 atctaggtcc aaatgattgt catatgggta aaaaagagtc tgtatcagat acagcaaaag
     6301 ttctaggaag attctatgat ggaatagaat ttagaggctt taaacaagaa acagttgaaa
     6361 gtctagcaaa atattctggt gtacctgttt ggaatggact tacagatgaa tatcatccaa
     6421 cacaaatact ggcagatttt ttaacagtaa ttgaaaatgt agataaagat ttgaataaag
     6481 ttaaatttgt atatgttggt gatgctagaa ataatatggg gaactcactg atgataggat
     6541 gtgctaaaat gggaatacat ttcgtagcac tagcacctaa ggaactttgg ccaaatgaag
     6601 aattggttaa ggaaatgaag gaaatagcca agaaatccaa aggagatata atgttgactg
     6661 aaaatattga tgatgttaaa ggtgcagatg ttatatatac agatgtatgg gtttctatgg
     6721 gagaagagga gcaatatgaa gcaagaataa atcaacttaa agattatcaa gtaaatatgg
     6781 atatgataaa gaaaactgaa aatgaaaatg ttatattttt acactgtctt ccagcatttc
     6841 atgatttaga aactaaggtt gctcaggaga tttatgaaaa gttcggatta tcagaattag
     6901 aggttacaaa cgaggtattt aattcaaaat attcaaaggt atttgaagaa gctgaaaata
     6961 gaatgcatac aataaaagct gttatggttt ctactatgac tgatgtaaat atgaacaata
     7021 atttaatata gatatgtttt aatatagact ttactttaat gaaaatttat aaaatatttt
     7081 aaatatatta ttcaaaaact ctcattatat gttaaaataa cttatgtatc aacaaattaa
     7141 gagggaggat ttgagatatg aaaaaaatca tattagcttt aacaactttt ttattagcaa
     7201 tatctttaac agcatgttca agtgcaaaac cagaagatac aattgatagt ttttttaaca
     7261 gtgcgaaaaa gtttgacttt gaaggcatga ataaggtaat ggaaaataat gacgaaaaat
     7321 ataaggatat attaaaggaa ttagatacaa aagacccaaa tgcccaatat gttttagatt
     7381 atctaaaaca gaatgcatct aaaatcacat atacaataaa agacagcgag gttaaaggtg
     7441 ataaggctac aatcaaagta gaatgtaaat ttatagattc aacaccatta cttcaagaaa
     7501 tagtagctga ggcatttaca aagatgattg gtatgagttt ttcaggacaa gacttaactg
     7561 atgagaaaac aactgaaatg ttagtatcaa ttatgaaaga aaaacaaaaa agcgttaaag
     7621 agacttatgt tacaaaaaat gtagagtttg aatgttctaa aaaggataat aaatggataa
     7681 taagttcagt aaatgatgca gtagctgatg tattgttatc taatcttgtt acagcaggac
     7741 aagaattttc taactctatg aatacacttg aataatattg tttaggtgta gtaaagtaat
     7801 aaatatatga ttatttttat aaataaacat ataaatattt atgaaggata ccatttttgg
     7861 tatccttttt attaatataa tgaaatacat ggttaatttc tatattgtca agaaactatg
     7921 atatatgtta taagtctata ctatttaaaa taaacttata acatatattt tttataatat
     7981 agtgtatgaa aaatttattt aactaatttt agatatttat tgacaactga ttcagccaag
     8041 cattttgatg aatcaacaat atttattgaa ggatatgatt tttctaaaac tacatttaaa
     8101 tcagtacaag caattactat attttctata gattcatttt taacatcttc aataagttta
     8161 ttccaaatat caatattgtg tggattttct ttatccattt ttatattttg aataagattg
     8221 tttaatttta tttgccattc atctttaaat ataaattcat gtccattatg tattatacca
     8281 ttctggtaaa ttgtagactc aaaagttgaa ctagtcgaaa aaagagttac tttttgtgag
     8341 atagtaggta attttttaac tgtttcttct acaatattta atattggtat attaatagac
     8401 tcttttaatt ctttaaagta tatatgtgct gaattgcatg gcattgcaat aaaatttaca
     8461 ccagttgatt caagcttttg taatccatca attatagttt ctttcataag gtcatgattt
     8521 attggacggt caatataaaa tggggtaggt aatgaatata tcatcatctt tggaaattca
     8581 tcatcatatt ttgctccata ttgagattga cactcatcaa taattaaatc aacaaaaggg
     8641 gctgttgacc ttggacccat tccagccaaa ataccaatta atttttcatt cataaaaatt
     8701 atctccttta tatataaata tatttatgta taaaataaaa atctataaaa
//
LOCUS       DAYAOX010000151         8718 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.152, whole genome shotgun sequence.
ACCESSION   DAYAOX010000151 DAYAOX010000000
VERSION     DAYAOX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.152"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            131..301
                     /locus_tag="ACGI57_002715"
     CDS             131..301
                     /locus_tag="ACGI57_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFG9717784.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            complement(477..1016)
                     /locus_tag="ACGI57_002716"
     CDS             complement(477..1016)
                     /locus_tag="ACGI57_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFG9717785.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(1155..1787)
                     /locus_tag="ACGI57_002717"
     CDS             complement(1155..1787)
                     /locus_tag="ACGI57_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFG9717786.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            1923..3836
                     /locus_tag="ACGI57_002718"
     CDS             1923..3836
                     /locus_tag="ACGI57_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9717787.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            3852..5228
                     /locus_tag="ACGI57_002719"
     CDS             3852..5228
                     /locus_tag="ACGI57_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9717788.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(5368..6018)
                     /locus_tag="ACGI57_002720"
     CDS             complement(5368..6018)
                     /locus_tag="ACGI57_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFG9717789.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            6489..8501
                     /locus_tag="ACGI57_002721"
     CDS             6489..8501
                     /locus_tag="ACGI57_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9717790.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT         3293 a         1101 c         1211 g         3113 t
ORIGIN      
        1 ttattgtaaa actttaaaac aagaatatca taataaagtt tttgttgtag aattaattta
       61 attgttatat tcaataatat ttgttataat gttattgaat tacaatacat ataaaaggga
      121 ggatttcaaa atggcatata aaattactga cgcttgtata agctgtggtg cttgtgaggc
      181 tgaatgccct gttagctgta tatcagctgg agacgacgca tatgttatag atgctggttc
      241 ttgtatagac tgtggttctt gtgcaggagc ttgtcctgta gatgcacctc aaccagaata
      301 ataattcgtt attcataaag agactgattt tattttataa atactaatca gcctcttttt
      361 aattatgtat gattcaatta atattattta tatactttat tatgttgcta cctaattcat
      421 cttaaaatac tgttagcaca tatctaatac ttttaacttt aatctaattt ataattttaa
      481 tactcctcat aaaatctttc atatttatta aagcaatcaa gacatacttt ctttccttct
      541 tcaatcctta tatattgctc agatgcaaat tcaccacaca cttcacattt aagagattta
      601 aatattcttg ctttttcagg aagtcttaac ttagtatctg taatctcaaa tatttcttca
      661 aaatcacttt ctaataagta ctttttgtat ccttctctat ccatttgaaa tggctttgac
      721 tttacatata ttcttataga ttttccattt tccctattaa agaaatgata tactgatttc
      781 ccagtcatcc taagtagtaa atttccttta cccattgtgc aacttagcac gacttgtata
      841 ccatctattc cacaagcatc attttcagaa atacatacaa tttcctcatc tcctgactgt
      901 ttacatccta aatattctct tgctgcaagt gctgctttaa atcctatagc taaccctgga
      961 cattcatgac catgaaattc aattgccttt ttccataatt cctccacaaa aaccactctc
     1021 cttaatattg tttatttcca tacattattt aaatttttat cctaatattt caaaaagcca
     1081 gaggatttaa tattaaatct tctgactttc tcaatactct tcacaactgc tcattattaa
     1141 tatgtttaag ttgtctactt tccttctttc attaaatacg ataaggtaaa taaactttct
     1201 gttaagttta cattttcaac tataacacct ttcggaactt ctaaatctaa aggtgcaaaa
     1261 ttccatacac ctttgattcc agcttttaca actctattaa taacttcttg tgctccattt
     1321 ttaggtatac ataatacagc aatatctata ttattgtttt ttataaagtc ttctaaagta
     1381 tctgaatcta atacttcaaa ctctcttatc tttaaaccta tcattctagg atttgcatca
     1441 aataaagctt ttatctcgaa tccagctttt ctaaatcctg cataattggc tatagcttgt
     1501 cctaagttac ctgctcctac aagaactgcg ttgtatggtc gatccaaccc aagaatttta
     1561 cctatctcag tatgaagagc ttctacatta taaccatatc cttgttgtcc aaatccacca
     1621 aagttgttta aatcttgtct tatttgagaa gcggtaaacc ctattatatc actcaattct
     1681 ttagaagata ttctttgtat atccctatct aataagtctc caagatatct atgatatttt
     1741 gggagccttc ttataactgc cattgatata tttttatttc ccaacatctt cacctcctaa
     1801 aagtgtcctc ttttttgttt gattcacaat aaaatatttc tttttcaaaa ctaatacata
     1861 taaccttgat tatattatat caaaacatta taattttaga tagttcaagg aggtagaaat
     1921 ttatgattgt tttgtcatgt aataacttaa ataaaagttt tggtatagat tctatattag
     1981 aaaatattag ttttacagta aatgaaggcg ataaaattgg tataatcggt gttaatggta
     2041 ctggaaaaac tactttattt aaaataattt caggaattta tggatatgat agtggagata
     2101 tttacacttc taaagattgt gaaatcggat atttagaaca aaataccaat ttttattcgg
     2161 ataatactat tttggaagaa gtactagaag tttttaaaaa tttgatagaa atggaatcct
     2221 atttaagaga attggaggtt aaaatatctg aagaaagtac aaaaacaaac tctccaatta
     2281 tagaaaaaat catggatgaa tattcacata aactagaatt attttcagat ttaaatggat
     2341 atggatataa atctgaagcc aaaggagttc taaagggatt aggatttagt gacaatgata
     2401 tggataagcc aataagcata ctttctggtg gtgaaaaaac tagagttctt ttaggaaaat
     2461 tacttcttaa gaaaccaact ctattgttac ttgatgaacc tactaaccac ttagactctg
     2521 aagcaataga atggttagaa gtatttttaa aacaatataa agggactgtc atgttaatat
     2581 ctcatgatag atatttctta gaccaatccg taaatagaat ttttgaagtt cacaacaaaa
     2641 aacttaaagt atataatggt aactactcta aatttgttga gttatctaaa atagaaaaag
     2701 aactagagct taagaaattt gaagaccaac aaaaagaaat aaaaaagcaa gaagaatcaa
     2761 ttgaaagact aaaagcttat ggtagagaaa aacacctaaa gcgtgcaaga agtaaagaaa
     2821 aagcattaga caaggtagac gttttagata aaccagaagc ttataggaaa aaagctcgta
     2881 tacaatttac gccttctgtc cagagtggta atgatgttct tcaaattaga gatgtatcta
     2941 tgggttatgg agaaagaata ctttttaaag acttggattt agatatatat agaggggaaa
     3001 aagttgcact tataggtgca aatggtgttg gcaaatctac attatttaaa ataataacta
     3061 atgaactaca acctcttagt ggcaatatta aatttggaac taatgtacat gtttcctact
     3121 tccatcagga acaaaaaact cttaatttag ataacacaat aattgatgag atttgggaaa
     3181 ataatactca tcttactcaa actactttga gaaatatgtt aggggcattt ttatttgtag
     3241 atgaagaagt ttttaagaag atttctacat tgagtggtgg agaaagagct agagttgcca
     3301 tacttaaact tatactatca aatgcaaact tattgttatt agatgagcct actaatcacc
     3361 tagatattga ttctaaggaa gtccttgaag aggcattaac taattatgat gggactatat
     3421 ttacaatatc acatgataga tactttctaa atactgttgt tgataaaatt ttagttttag
     3481 atgaaaatgg aattactgaa tatctgggaa attatgatta ttatatagat aaaaaaaggc
     3541 aaatccaaga gatgagtatt atagaagaaa aagaagaaaa aactagaact caaataaaag
     3601 atgaaaaaag aaaagagaga gaacaaagag aaatagaaaa gaaaaataga ataaaaagac
     3661 aaaatataga aaaagaaatt gaaaagttag agattgaaat tgaaaaatta gatatacttt
     3721 tatgtcaaga agaagtatac tctaatccag ataaagctaa agaagttagc caagaaaaaa
     3781 ttaatttaga aaataacctt gcctctctat atgatgagtg ggaagaattt atgtaaaaag
     3841 gagaggatta tttgcaagaa ctattttctt taaaagacga aaataaaaga ttttataaaa
     3901 tacttctatc tttatgtata cctattatca tacagaactt aatttctact tctgtaaatg
     3961 taatagatac aattatgata agtagtttgg gggaaacatc tgtagcttca gttggagttg
     4021 caaaccaatt tttctttctt tttaatatgt ccttatctgg tatcacagga ggtgctggag
     4081 tttttatttc acagttttat ggaaagaagg atgtgagtaa tataagaaaa gtcacaggtc
     4141 ttacttgtgt attggcaatt gttttaagtt ttgtatttgt tattcctgct ttactaacac
     4201 caaagccaat tatacacata ttttcgtatg attctgaggt ggtaaaactt tgtatagatt
     4261 actttagtat tgcagtattt agttatccat taattgctgt aagtactgta tttagcactg
     4321 gttcaagagg tgttagaaat cctaagttag gtatgatttg tagtgcattt gcacttgtaa
     4381 caaatgttat tttaaattat ggatttatat ttggtaactt cggtcttcct gcattgggtg
     4441 taaaaggtgc tgctctagct actgttattg ctagaatatg tgaattaatt ttaatgatta
     4501 cttatgttta cctttataaa aaagactata tattaaagtt tggtctaaaa aatctaaaag
     4561 ctattgataa aatttttata aaatcttttt catcaaaaag ctttcctata tttgtaaatg
     4621 actctgtatg ggcaattgga actgtcctgt attctgttgc atatgcaaga gctggtactt
     4681 ctgctatagc agctagccag atagcaacca gtacaggtaa tttcttcata atgacagctg
     4741 tatgtattgc atctggtgca tctattatgc ttggaaatga acttggagct gaccatatta
     4801 aaagagccat tgaatatgct aaaaagtttt ctatacttgt attttcagct ggtttaatac
     4861 ttggaattat tttaatttta aatataccat tattattaaa aatgtttagt gtttctgata
     4921 gcttagcttc tgatataact aagatattct ttataatggg aatactaatg gctcttaaat
     4981 cttttaatac attggttata attggtattt taagaagtgg aggagatact aaatatgctc
     5041 tatttttaga attaggatgt atgtggttag cttctattcc tctaactttt atagctgcat
     5101 ttaaaggtgc acctatattt gtactcgttt tgctcactta tagtgaagaa gtggttaagt
     5161 ttatatttgg tgttcctaga gcattatcta aaaaatgggc tattaatata gtaaaggaaa
     5221 ttgattaata attatactat acattataag tatacaatat ttctaaacag tctcattgaa
     5281 aataactaaa gtatttttga ttttcagtga gactatttct attttttaca gaaattatta
     5341 tactttattt tgattaagta acaattttta ctcatcttcc aaaaattctt ttacttcaaa
     5401 tgccaattta tgtaattcat catagtttaa aatttcatat gtattaccca ttttttttac
     5461 tatctccttt tcagttaact ttagcagaga atggtatcta ctttttctac taattgaata
     5521 gatttcactg aaattacgaa tagcttttat attgcagaca ttattttctt gtttatttga
     5581 tatacaatag gcaataactt ctgtgcaaga aaatagttct cttaccattc tggttttata
     5641 taattcatat attcttcttg tacattttct gtgaaatagt gcaataaaat ctggtttgag
     5701 ataaaactct tcaaatacat ttttttcaaa tcgaaataat tttatattgg tctttgcaaa
     5761 tatatcacat ttcaaattat gttcatatat gtaactaatt ttgcctataa attcattttc
     5821 aggtacaatg tcaacaagaa atttatttcc tcctctagta gtacactcca ctaatgcaac
     5881 tccctctatt ataaagaaca cttcttgtaa gtctttatct gatgctacaa atctttgacc
     5941 ctttttatat tcaacaattg atattttgtt taaattattt ttaataattg gatatatact
     6001 tgttttttct gctttcatca aagttacctc ctttacaata tattgtataa aatattttat
     6061 ttatatgttt taatttatat tatttttaat aaaagataaa attacaacta aattaaactt
     6121 gacaaattca ttctactgac gcagaataaa cttatacata tatattttaa tatataattt
     6181 attttaatat aattctatat ttacaaaatt atattatcaa tagaatttat taaaataaat
     6241 ttttttgggt gtatattata atttaatcga atagaaatga aaaatataca tacagtttaa
     6301 gttttaagca acaattaggg ggatttggtg acaagatttt taattataag aaagaaagtg
     6361 tcttaatgca acttttataa agtcattata gacactttct tttttatttg ataaatttaa
     6421 attatcaaaa ttatattgct ttatacaact attttgttgg aaactattta ttatataagg
     6481 agaaataaat gaaaaaatca gtagctctgg taaatgatag tagaaaagat ttaatagact
     6541 ttttagaaaa taatttgaaa ttagtttttg gagattctat aaatattaat agatacttca
     6601 taaatgaaat aaatgataat gatattataa atgatgatgt gatattagta atgtcagtag
     6661 aaagattaga caaaattata aataatatac tagataagaa aaaagtgatt gtggtaagaa
     6721 gaacttttag ggaagataaa atttataatc tactatcttt accacagggt acaaatgttc
     6781 taatcgtaaa tgattctgat gaaactactc tagaaactat aagtttattt tataaaattg
     6841 gagtgactaa tattagacca attccatata tgaatgataa taactataaa aatataaaaa
     6901 tagctataac tccaggagtt ccagaaaaag tacctagctt tatctcagat atatttgatt
     6961 taggtcacag atatatagat atttctacat ttatagaaat aataaattta ttacaaattg
     7021 attcaaagga aatacaatct aatctagtta aatactcaga agaaataata agtttagata
     7081 ctggtataaa agataaatat aaggaattat ttttaaaaat agaagaatta gatacaatat
     7141 tgaatttatc aaaagatggt atattattta cttcaaaaga tggtgaaata aatacatata
     7201 atagtaaagt aaaagatatt ttagacataa atgaggatat atatggaaaa tacattgagg
     7261 atatatttgt agatagttta aaagttttat tgagtgagaa agagatacta gataaagttg
     7321 tcgtattcaa taagaaatat ataaatgtaa ataaaaaaaa catatacaat agagatgaaa
     7381 aaatgggcac atattatagt ttacaagaaa taacatacat aaaaaaatta gaacaaaatc
     7441 tcactaaaaa attaagagaa aaaggacaaa tagcaaaata tacatttaag gatataaaaa
     7501 caaatagtcc taaaatgttt gaatgtatag atttagctaa gaaggtttct aaatctgact
     7561 taagcatact tatcagaggt gaaagtggaa ctggtaaaga actcatagct cagtctatac
     7621 ataacaactc aaatagaaaa aatcaaccct ttatagcagt aaattgtgca gccgttcctg
     7681 aaaatttatt agaaagccag ctattcggat atgacaaggg aacttttact ggaggtctaa
     7741 aagatggaaa acaaggatta tttgaacttg ctaataatgg aacaatattc ttagatgaaa
     7801 taggcgatat gcctttagaa ttacaaacaa aactacttag agttcttcaa gaaaagcaga
     7861 taatgcctgt tgggtctcac aatgtaatta atatagatgt tagaatcata tctgctacaa
     7921 ataagaattt agaacaaatg attgataata gccaatttag agaggattta tattatcgat
     7981 taaatacaat tccaataaat atacctcctc taagagaaag aaaagaagac atattaatta
     8041 ttatggaaga tttaattaat aagaaattag taatcacacc agaagcaaaa aaacttatac
     8101 aaaactatat gtggaaaggt aatataagag aactccaaaa tgttacatcc tatttaaaca
     8161 ttatgtgtga agatattgta ttagagaaag atttacctcc aaacttaagg tcttcagata
     8221 acaaaaatac atcattaaaa ctaaaatata gtaagaatga tatattgaat atattagaga
     8281 tactaatttt aaataaggaa tcagatgttg gtattggtag aggattaatt ttaaaagcac
     8341 tactcgataa gaaccttcaa attacagaag gaaaaattaa aaaaatattt gaatatctaa
     8401 aaaaagaaga acttataatt tgtagttcag gaagatatgg aagtaaaata actcaaaaag
     8461 gagaagattt ttacaataaa ttaaaatata aaggtctata aaattataat ttatcaaaca
     8521 aagaaatcta ttcaggatta aagtactcct tcaataggtt tttttatttt tcaagtaccc
     8581 tgaaattata atttattttg tacataaata ataggaatta ataggaatag gataattttt
     8641 ttatttccta ttttttttat ataaactagc atataaaata tattttcttc aaaataatat
     8701 tttttactga aaattgaa
//
LOCUS       DAYAOX010000152         8684 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.153, whole genome shotgun sequence.
ACCESSION   DAYAOX010000152 DAYAOX010000000
VERSION     DAYAOX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8684
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.153"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     repeat_region   90..570
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=156..178
                     /rpt_unit_seq="ataccacttagttaatataaaac"
     gene            complement(1100..1204)
                     /locus_tag="ACGI57_002722"
     CDS             complement(1100..1204)
                     /locus_tag="ACGI57_002722"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFG9717791.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            1829..2074
                     /locus_tag="ACGI57_002723"
     CDS             1829..2074
                     /locus_tag="ACGI57_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717792.1"
                     /translation="MNQKKIKISIKMVIIGIFSVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIIFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(2571..2705)
                     /locus_tag="ACGI57_002724"
     CDS             complement(2571..2705)
                     /locus_tag="ACGI57_002724"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717793.1"
                     /translation="MLDKRLGKRRLKNIYEDIEKENNLVKKFYNIRVESENMILKKNA
                     "
     gene            complement(2827..2973)
                     /locus_tag="ACGI57_002725"
     CDS             complement(2827..2973)
                     /locus_tag="ACGI57_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717794.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            3069..3392
                     /locus_tag="ACGI57_002726"
     CDS             3069..3392
                     /locus_tag="ACGI57_002726"
                     /inference="COORDINATES: protein motif:HMM:NF024502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFG9717795.1"
                     /translation="MKDRECSFSPNTIRNRKNMIKNQIEVFFKDMKLIDITPSILQSY
                     INNIYNEHMLNSIKNQVDFIKSVLKESYRLKEISENICDFVTTPIKKVVQLLSFIQNK
                     KLNYY"
     gene            4259..5614
                     /locus_tag="ACGI57_002727"
     CDS             4259..5614
                     /locus_tag="ACGI57_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9717796.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(5732..6391)
                     /locus_tag="ACGI57_002728"
     CDS             complement(5732..6391)
                     /locus_tag="ACGI57_002728"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HFG9717797.1"
                     /translation="MLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTISEEH
                     GKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAYGDI
                     DIESHRKWDTDRCEAVGMKVDLPLWQESREELVYEFIDSGFCSVVTKVNLKHLGEEFL
                     GKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGYLEI
                     K"
     gene            complement(6467..6895)
                     /locus_tag="ACGI57_002729"
     CDS             complement(6467..6895)
                     /locus_tag="ACGI57_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9717798.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            7047..7595
                     /locus_tag="ACGI57_002730"
     CDS             7047..7595
                     /locus_tag="ACGI57_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HFG9717799.1"
                     /translation="MYTNFNIDFNNFNKKENAIKFMLMGVLLIILGLLCLTFKTLGIK
                     LISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVLIIACILLFVFPQKIQ
                     SIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGICLILSPLFLSRFIVSI
                     MSFFIILFGLVLFFSGNTARKY"
     gene            7714..8649
                     /locus_tag="ACGI57_002731"
     CDS             7714..8649
                     /locus_tag="ACGI57_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFG9717800.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNKIDDTLKARNSYQGCKFV"
BASE COUNT         3166 a         1181 c         1037 g         3300 t
ORIGIN      
        1 taatttatct atataaaaaa gtgctttgtt gacagagttc acttttttat ataggtacag
       61 aatttaagta taaatacatg ttttattcta tacattttag ttaatgtaaa atttcttgat
      121 tgtactctct aacttttcaa cattatacta tttatatacc acttagttaa tataaaacta
      181 taaaagtgtt gcaattactg ggttttataa agttaattta cataccactt agttaatata
      241 aaactttagc accttattta tctatagagc tacagaaagt atttacatac cacttagtta
      301 atataaaaca tgtatttatt tgttcttgta ctattgtttt actctattta cataccactt
      361 agttaatata aaacaatatc aaaacaaata aaaattttta ataaatcatt atttacatac
      421 cacttagcta atataaaaca ttctggtact gttgctattt atgttccgta ttcagaattt
      481 acataccact tagttaatat aaaactttgg agttgttaca gattatttgc cttatctctc
      541 tatttacata ccacttagtt aatataaaac ccccaaataa actgggcatt ttcaatactt
      601 acacatatgc aactctctta aatttgcagt gaaccatgag tagtgcaaat gataaagctt
      661 atcatatacc ctcaacacct tatatttcaa gcgttaagct ctactttgta acaaatatcg
      721 ctcactgcaa aactatttac atttttatta tatcataaat atattatttt tgaatatcta
      781 taccaatttg tggtataata aaagcaagaa gaactacaat ctatttagac gtagagtgaa
      841 gttcaaatac taatgtttat tctttttgaa cttgattttt agttcaaaac taaagtcact
      901 cctgcctgtg tgaactatac ccgctgtttt atacagttaa aacatgtaaa aatagttata
      961 aagtactagt gtaactcatt agtactcttt ttattatatt catataatta atatctatat
     1021 tttaaagctt actatctaaa aaagagcacg tcaaataaaa ccctagatat ttaatttttt
     1081 tagagatact taaaataaat tatgctctct ctgtacttaa atatttccta aattcaaatg
     1141 gagacataga atttaatcta ttttgatatc taaaattatt atagtagtat atataatcat
     1201 ctattgcttt aactaattat tcatatgtat caaatttcct taaatagtac atttctacct
     1261 ttaaaattcc ccaaaatcct tccataggag cattgtcaag gcaacgacca actcttgtat
     1321 aatatatgta tatctaacac cattaagaaa tatatatttt ataatatata tttcttcatt
     1381 taatgcactt agaaaagcaa agaaaataca gattagtaca ctttttagag aaaaataaaa
     1441 ttaatacatc taaaatatta aaaaatactt tagataatct aaagacacat ctcaaagttt
     1501 ttaactaaat tataaaacta attacaaatg ctatgtaacg ataataaata aaaaatttac
     1561 ttcgcttaag cgacgtgtcg ttgaaatatt tcatgttgcc aactgattca taagtcgata
     1621 agaaaagaac actaattatt tagagttctt ttcttagcta caagtggatt ataatacttt
     1681 taaatctgtg agaaaatcat atataataat gatatgaaca agtaaagaaa atacaaaaat
     1741 gacaaaatta taaataaaat attataaaat cataaggtca taattgttat attgtagaaa
     1801 caaagtaaag agagagggtg gagaataaat gaatcaaaaa aaaataaaga ttagtattaa
     1861 aatggtaatt ataggtatat tttctgtaat tattgctttt gtattatcca gattattcgt
     1921 tgagtatatt gatgaattat taaggtctaa ttggcaatat cagctatttg aaagtgagag
     1981 ttcatatata atatttgcca attgtattat gttctcgaat atattattag aaatattttt
     2041 aatatatatt tgtagaaaat ttagaaaaat ctaatatggt caacgtattt cgaatcgaat
     2101 acttcatgat tatttagtct attgtgctag ttaaagttta attcccatat aaagtaaatt
     2161 gattttagta aaacaaatca cccctaaggg ttagtttatg gataagtcat ccaaatatta
     2221 actctttaat ttttgctata gattcatctt tgctcataac tttacttata aaaaatgaaa
     2281 tattataagc tagaactttt attctaattc tagtcataaa tcctagcata gagttacttt
     2341 ttttatatta taaataaaac tatacaacaa agataatatt tagtaaaaat aactttcatt
     2401 ttaactaaac ttaaaattca attactctct aaattgacgt ttaggaatta gttcaaaata
     2461 aaaaagagtg tatatattga aattactaca tttcaatata tatactcttt ttaaatttct
     2521 cagttttcca tggaacttta ctttatttat ttctgtatta gttgtatttt ttatgcattt
     2581 ttctttaaaa tcatattttc tgattcaact cttatattat aaaatttctt tactaaatta
     2641 ttctcttttt ctatatcttc atatatattt tttaatcgcc ttttacctaa tcttttatct
     2701 aacattacta atgatttaac taatatatta tatgatttta tcgcatcttc tattgagata
     2761 tttaaacata tatttactgc ttcataaaaa tcaaattcat taaaaaatcc atttttattt
     2821 acaatcttat atccctttgc atctctttct aaatactctt cctaaatact cttcaaaact
     2881 ttcatcatct aatctaaatc ctatatcact atgaaaaata ggttttgcat ttggatatag
     2941 ttctattgtt ttatcaaaag tttcgaatac caagttatta ttattcgatt ttccaatagt
     3001 ccactataaa caataataag tttgttgctc caaataatgt aacacttgta gaaagatgtt
     3061 acagatatat aaaagataga gaatgtagtt tttctcctaa tactattaga aatagaaaaa
     3121 atatgattaa aaatcaaatt gaagttttct ttaaagatat gaaattaata gatataacac
     3181 cttctatttt acagtcttat ataaataaca tatacaatga gcacatgtta aattcaataa
     3241 aaaaccaagt cgattttata aaatcagtat tgaaagaatc ttatagatta aaagaaatat
     3301 cagaaaatat ttgtgatttt gttacaaccc caatcaaaaa agtagttcaa cttctaagct
     3361 ttatacaaaa caaaaagctc aattattatt agaaaaatca aaaaatattc ctataggtat
     3421 accaattttt ttgatgctaa ctcttggatt acgttttggt gaagctgtat cacttatatg
     3481 gtcagatgta gaccttgata aaaaatctca catgtaaatc aaacattagt ttatgtatac
     3541 aataaaataa tcttcaaaga ccctaaaacc cctatatcaa aaagaaaatt atttgctcct
     3601 gatgaactta taagtttgct taaaggggaa aaattaaaac aaaataagtt aaaattacag
     3661 ggtatattga aaaatgaatt tgatttgata tgcttaaata aaagattcaa cccgtgagtt
     3721 caaccaactt ttgttgttca ttttaaaaaa ctattaagag ataataatcc tagagatata
     3781 aaagtacatg aattaataca tgcaaatgca actttgatat tactttcagg tactaacata
     3841 aaagctattt ataaaatatt agtacatgca gatataaaaa tagctatgaa tatatattcc
     3901 atattctcac ttattagatg aaatgaataa agaaacatct gaaaatataa gcaaaatact
     3961 attcaaataa tgttcagttt tatgtcaatt aaatgtcact caaagtgctt ttttttcatt
     4021 tgtcagttaa atgtcactta aagtaccttt ttcctatttg tcagttaaat gtcagtaata
     4081 tataaataag atatatttat aagaccctta aaatcactat tatttaataa aatttataat
     4141 tatatactat ttgctgttga cattggtatt ataccatagt ttatcattta tattagaaat
     4201 aaaattatta attaatagtt tcgtaatttt ataatagatt tacagggagt gatagtaaat
     4261 ggatacaaaa atatctataa acgaaaacat gagtctaggt aaaagatttt ttaaatacct
     4321 agcaccatct gtagttgcaa tgtgggtttt ctcactatat acaatggttg atggaatatt
     4381 tgtaagtaaa ggagttggag aattagcttt agctgctgtc aatatatcaa tgccttttat
     4441 taattttata tttgcagttt ctctattatt ttcaactggt gcatctacca taattgcaat
     4501 ctatctgggg aaaaaagata ttaaatctgc taacgaagtc ttttcattta acttagtatc
     4561 aataattata ttatcaataa ttatattagc tataactttt tttaatttag atagattagc
     4621 actattttta ggagctacag aatctacaat tggtatggtt aaagattatt taggaataat
     4681 aatatttttt aacggattct ttatagtgtc ttattcttta gaagttataa taaaaactga
     4741 tggctttcca atattagcta ctgttggtgt aataatatct gctcttacaa acatcatact
     4801 tgattacttg tttgtaattg aatttggatg gggtgtaaaa ggtgctggta ttgcaactgg
     4861 tttgtctcaa gtcttttcta ctattttctt cttaatacat tttttaagaa aaaactctac
     4921 tttgaatttt tcaaaattta gaattgattt taagacactt agaaaaattg tatttatagg
     4981 ttttcctgat tcaactactg aattaagttg tggtatagtt gttttacttt ttaacttaag
     5041 tcttactaaa tatattggag aaaatgctct tatttattat agtgtcataa attatataaa
     5101 tacattagta cttatgacta tgatgggtat aactcaaggt atgcaaccac ttactagctt
     5161 ttattatggt gcaggaaata tagataatgt aaaaaaatta ttaaaaatgg ggataaaagc
     5221 cacaataata gcatcagttg ctgtatttgc aatttgtatg gctttttcag gaccaatagt
     5281 ctctttattt atacatccag aagaaacaat gctattcaat gaaggagttc gtgtatttaa
     5341 aatattctct atatctttct tattggtagg aattaatgta ataatttctg gattctttgt
     5401 atcagtagaa aaaccatcaa tttctactgt tatatctctt ggtagagggc ttgtaatagt
     5461 tgttttaagt ctaatttcaa tgatactaat atttggtgga caaggaatat ggatgactac
     5521 tattgtttct gagtttatat gtcttatttt atcattagtt tttctaaaaa agaatttttc
     5581 tacattagac tctaatctaa ataaggttgc ttagatgata agagtgacaa tatatgaaaa
     5641 tatctaaata aaaaatacct attatacaat cttgcaaata ttaaaccttt gcaaaaatta
     5701 ggattatata ataggtattt tatttatgta attattttat ttctaaatat ccatagccat
     5761 cttttattaa agttcctttt acttcatatt ctacaggttt tttaaatatt ggaccatcta
     5821 cgacaaatgt atgatactct ccatgttctc cacatgggtc tgcaccagca ttttttatct
     5881 tctccacaag ttctctagtt aatttttttc ctaaaaattc ctctcctaag tgctttaagt
     5941 ttacttttgt tacaacacta caaaatccac tgtctataaa ttcatatact aactcctctc
     6001 tactctcttg ccataatggt aaatctactt tcataccaac tgcttcacat ctatcagtat
     6061 cccattttct atgactttct atatctatat ctccatatgc acatatagtt gctcccatgt
     6121 ttttagcttt tattaaagcc ctctcaaagc tctcttcata tccttctaca ccacactcag
     6181 caactaataa aggtaatcct atattgctac ttacttgctt aagcatatta tattctaaat
     6241 tatgtgtcca tgactttcca tgttcttcac ttatagttgt caagagtgcc acaggcttat
     6301 atcctttttt caacattcga tttaatgcta atatactatc cttaccacca ctaaatgaca
     6361 ttacaaattt ttcatcagcc aattttaaca cctctttcat ctaactttgt tttattaaca
     6421 taattatacc aaatttttga gttgcatagc aaattatatt aaattgttaa ttcactataa
     6481 agaatgtccc taatattaaa agtataaccc ctatgtattt gtataaatgc attgtttctt
     6541 gtcccaaaat tacgctatca ataaaaaatc caactaaaac ttgggataca agagttaata
     6601 taagtgtatt tgatactcct aaatttggaa ttgcctttac tgtaaaatat attattagac
     6661 ctccaaatac tcctcctaat aacaacttat aattaaaacc atttatagat actagacact
     6721 ttaagtcacc tgtaactatt atacttgcca aaaaaaatat tgctcctaca gttaagctta
     6781 taaaggtggc cactaatgct gttgtacttt ttcctagctc tccatttata aaagcttcaa
     6841 gagctgtaga accacctgct aataatgcaa atattattgc ccataatttt gtcatggaca
     6901 aacctccttc tatataattc tattaataga agtttatgat acaacctatt aattaattac
     6961 taagtatagt ttaattttta tacactaggc tttatttgga tatgaatttc tgttataatt
     7021 gttctatcga ttttggaggt gattttatgt acacaaattt taatatcgat tttaataact
     7081 ttaataaaaa agaaaatgcc attaaattta tgttaatggg agtattactc attatattag
     7141 gattattatg ccttacattt aagacattag gtataaaatt aatttcttgg acttttggta
     7201 ttgcactttt attctttgca tatttaaatt taaaaaatat taatgaacta aaaagatatg
     7261 ctactaaaga agaaattaaa ccctctatta atatccaatg ggttctaata attgcatgta
     7321 tattactttt tgtatttcct caaaaaatac aaagcatatt ttcattattt ttaggatttt
     7381 atttaatctt taatcaacta gtagcacttg taaatagtaa gaataatcct tattctaaat
     7441 ttacaacttg gaatatagtt aagattctat ttggtatatg ccttatatta tctccacttt
     7501 ttttatctag atttatagta tcgataatgt cttttttcat aatattattt ggactagttc
     7561 tatttttctc tggaaataca gcgagaaaat attaattatt ttctcgctgt atttttatat
     7621 tacttaaaat agaatttgtt taagttatct atttgtggta atatgttttt tgtacaagct
     7681 cagcgtacaa ttttattaga aataggtgat tttttggata aatttaaata tgcttttgat
     7741 aataaaagat atcatacttg gaattactat cttagaaata cttttggaga aaaagttttt
     7801 aaggtatcta taaatgcagg atttacttgc ccaaacattg atggaagtct tggttatggc
     7861 ggatgtacct attgtagcaa agaaggctca ggtgattttg ctggaaaccc taaagataat
     7921 cttatcagtc aattttataa tataaaagaa atgatgttaa aaaaatggcc tcatgcaaag
     7981 tatattggct actttcaagc ctatacaaat acgtatgctc ctcttgaagt tttaaaagaa
     8041 aaatatgaga ctatattaga acttgaagat gtcataggtc tttcaatatc cacaagacct
     8101 gactgtttgc cagatgatgt tgtagaatac ttgtctgaac taaataaaag aaccaattta
     8161 tgggttgaac ttggacttca aacaattcat gattctacat ctaaaattat aaatagaggt
     8221 catgattata aaacatttgt tgaaggtgtt gaaaaattaa agtctaaaaa tataaaggtt
     8281 gtcgtacaca ttataaatgg tcttcctggt gaagattata atatgatgat ggaaacagca
     8341 aaggcagttg gtaaaatggg tgtagacggt ataaaaatcc atctattgca tgttataaaa
     8401 gatactccta tggaaaagat gttacaaaat ggtatgttaa ctcttatgga acaagatgaa
     8461 tatatcaatc ttgtctgtga ccaattagaa atacttcctg aaactatgat aatccataga
     8521 ctcactggtg atggtaagag ggatgaacta gtaggtccta tatggagcct aaaaaaatgg
     8581 gaaattttaa ataaaattga tgatacttta aaggctagaa attcttatca gggctgtaag
     8641 tttgtttaat acaaaataat aactatagaa atggttatac aaat
//
LOCUS       DAYAOX010000153         8585 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.154, whole genome shotgun sequence.
ACCESSION   DAYAOX010000153 DAYAOX010000000
VERSION     DAYAOX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8585
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.154"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            146..1651
                     /locus_tag="ACGI57_002732"
     CDS             146..1651
                     /locus_tag="ACGI57_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFG9717801.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            2041..3138
                     /gene="dinB"
                     /locus_tag="ACGI57_002733"
     CDS             2041..3138
                     /gene="dinB"
                     /locus_tag="ACGI57_002733"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFG9717802.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            3401..4537
                     /locus_tag="ACGI57_002734"
     CDS             3401..4537
                     /locus_tag="ACGI57_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717803.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            4530..6077
                     /locus_tag="ACGI57_002735"
     CDS             4530..6077
                     /locus_tag="ACGI57_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9717804.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            6412..7278
                     /locus_tag="ACGI57_002736"
     CDS             6412..7278
                     /locus_tag="ACGI57_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFG9717805.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            7419..8561
                     /locus_tag="ACGI57_002737"
     CDS             7419..8561
                     /locus_tag="ACGI57_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFG9717806.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
BASE COUNT         3310 a          915 c         1544 g         2816 t
ORIGIN      
        1 aactatcttg aaaaaaatta atcttaattg tataatatta ctttgagtga aaaagtatta
       61 acattttttt ataagatgtt ttactaaaaa aatatatata aatatactgt tatcaacaag
      121 aaatttaatt tagtgaggtg ttaagatgaa agtaggattt gaccacaaaa aatatttaga
      181 agagcagtct aaatatatct tagaaagagt aaataatttt gacaaacttt atttagagtt
      241 tggaggaaaa ttgatgggag accttcatgc aaaaagagta cttccaggat ttgatgaaaa
      301 tgcaaagata aaagtattac aacatatgaa agaaaaagtt gaagttgtaa tttgtgtcta
      361 tgcaggagat atagaaagaa acaaaataag aggagacttt ggtatcactt atgatatgga
      421 agttttaaga ttaatagatg atttaagagg ttatgaacta gatgtaaata gtgtagtaat
      481 tactagattt gaaggacaac ctgctacaac tgtatttata aataaattag aacgtagagg
      541 aattaaagtt tataagcatt atccaacaaa gggatatccg tcagatgttg agactatagt
      601 tagtgatgag ggatatggag caaatgaata tatagaaact acaaagccaa tagtagtagt
      661 cacagcacca ggacctaata gtggaaaact tggtacttgt ctaagtcagt tgtatcatga
      721 aaataaaaga ggtaatgtag ttggatattc taagtttgag acattcccag tttggaatgt
      781 acctgtaaaa catcctttga atatagctta tgaagcagct acagttgatt taaaagatgt
      841 gaatatgata gattcattcc accttgaaac gtatggtgaa atgtcagtaa actacaatag
      901 agatttggaa ttatttccag tgcttaaaaa aataatagaa aagataactg gaaaagaatc
      961 aattttccaa tctccaactg atatgggtgt taatagagtt ggctttggaa taatagatga
     1021 tgaagtaata agaaaagctt ctatagaaga aataataaga agatacttta aaactgcctg
     1081 tgagtataaa aagggacaag ttgataaagg tgcttatgat agaatgaaaa tgattatgga
     1141 agagcttaac ttaaagccag aagatagaaa tgttgtatta cctgctagaa actacagtaa
     1201 taaattgaaa gaaagtgccg acaagaatga tacttgtcct gtagttgcat tagaattaga
     1261 agatggtaca atattaactg gtaagggttc tgatttaatg aatggaactg cagcagtagt
     1321 attaaatgcc attaaacatt tagccaatat atctgatgat atgcacttaa tatcacctgt
     1381 aatattagaa cctatcataa acttaaaaac aaagacttta gctagtagaa atgtttcact
     1441 tagttgccaa gaggtgttaa ctgcacttag tatttgtgct gtaactaatc caactgcaca
     1501 atttgctatg gaaaagttag cattattaaa agggtgtcaa gctcattcta ctactatatt
     1561 aaatagagat gatgaacaaa catttagaaa acttggtata gatgtaactt gtgacccaga
     1621 atatccttca caaaatcttt actatagtta aaattatata aaaaaatatg ggctgtttta
     1681 aagtaatagt ttgagcagtc cattacaatt ataataaaat cagtaggctg tcttgtataa
     1741 gacagtcttt tgtttttaaa acatcccata aattaaatat atatactgtt atccataagg
     1801 aattttaaat ttagttcctt ggaataccta ttgtaaagct tttatatagt ttttgcaagt
     1861 gtttttaagg attttttata aattatattg aagttaatgg attatagatg gttttattta
     1921 tggaaaatat gaatatataa agaaaataat gaaaaaatac caaaaagaaa cataagtttg
     1981 gtataattaa aaagtaatat aaataaaatt aatctattta tactttaaaa ggtgataata
     2041 atgaaacatt atagaaaaat aattcatata gatatggacg ctttttatgc atctatagaa
     2101 caaagagata ataaaaaatt aaaaggaaga cctgttattg tcggtggaaa tccacaaagc
     2161 cgaggtgtgg ttgcaacctg ttcttatgaa gctagaaaat ttggtatcca ttcagctatg
     2221 ccatcagctg tagcttacaa taggtgtcca tatgcagttt ttgtaagacc tagatttgat
     2281 gtttataaga gtgtgtcaga gcaaataaga gatatatttt atagatacac agatttagtt
     2341 gaaccattat ctttagatga agcttactta gatgtaacaa aaaataaaaa aaatattgat
     2401 tcatcaattg agatagcaaa gcaaataaaa aaagatatat ttagagaagt tggtcttact
     2461 tcttcagctg gtgtgtcata caataagttt cttgctaaga ttgcatcaga cttaagaaag
     2521 ccaaatgggt taactgtaat aaccgaagaa aatgcccagg actttttaga taaattacct
     2581 gtgaataaat tttttggtgt tggaaaagtt acttcaaata ctcttaaaaa tttaggtata
     2641 aaaactggat atgatttaag gtgtttaaat ttatttgaac ttgaaaatat attcaaaaaa
     2701 agaggttatg aattatataa atttgctaga ggaatagacg atagaccagt ggaaccaaat
     2761 agggttagaa aatctgttgg ggctgagact acattaagtc ataatttgga tattgatgaa
     2821 gaagaaacac gcaacattct tgatgaactt tgtgaagaag tatgtcatag attaaaaaac
     2881 tctgagaagt ttggaaaaac attaacatta aaaattaaat atgaggactt tacaaagata
     2941 actagaagcc ttagtttaga acactatatt gacgaatata atgatataag aagtggtgta
     3001 gataatttgt tgagaaatgt tgaggttaat ggaaaacaaa ttaggctatt gggagttaca
     3061 atatcaaacc taagtgataa aaaagaaact tataaagata ttacattatt tgaatacatg
     3121 gatagtatcc aaatgtgagt tttgtttaaa tgaaataaca ctagctttaa tactatataa
     3181 ttgaggctag tgttatttta tgaatatttt tatatttttt gttttttgac attgtatatt
     3241 atatactata tataaatact ttatatttgt agcaatattc agacaaatag tgacctaaaa
     3301 atcgtacttt gtttacataa ataaaatagt atgatataat tataaaaaca acttataata
     3361 ttggagaagg ttgcttgttc gaaaaagggg atgataaatt atgatagaga taagaaatgt
     3421 aacaaaaaaa ataggaaata atgttatatt agatgatatt tctctagttg tagaaacagg
     3481 tacattggta gttttgattg gttcaagtgg ttgtggaaag actactactt tgaagcttat
     3541 aaacaaatta ataaaaccta catctggtga gatatatatt aatggcaaac caatttctca
     3601 ggaaaatgaa atagagctta gaagaaaaat aggatatgtt atacagaaca caggcctatt
     3661 ccctcatctt acaataaaag aaaatataga gctaattcca cgcctaaaaa aggaaaaatc
     3721 cgttgaagaa attgaaaaaa gaacgttgca actacttgag atggtaggac ttgatagtga
     3781 tgaatttttg aataagtatc cttcagaact tagtggtgga cagcaacaaa gaatcggtgt
     3841 tgcaagagcc attgctactg atgctgaaat aatattgatg gatgagcctt ttagtgcttt
     3901 agacccaata acgagaacat ctttacaaga acaattattt tctcttcaag atgaattgaa
     3961 aaaaactata atatttgtaa cgcatgatat ggatgaggct ttaaagatag ctgataaaat
     4021 ttgtattatg aaggatggaa gaatagcaca atatgatact cccgaaaata tacttagaaa
     4081 gcctgcaaat gactttgtaa aagattttat cggagaagat agagtttggg ataatccaga
     4141 atatataaaa gcaagagata ttatgataaa aaatcctatt gctgttaatt ctactagaac
     4201 tgtaactcaa ggaatagaaa ttatgagaac gagtaaggtt gatagcttac taattataga
     4261 tagagcaaaa acattaaaag gtattgttac tgtaaaagat atgaaagata ttgatgataa
     4321 gtcaatatta cttgctgata ttatgtcaag tgagcctttg catgtaaatg aaggggataa
     4381 tttagtagaa atactaaatg taatgaatcg taattcggtt ggatatattc cggttatttc
     4441 tgatgaaaat aaactggtag ggcttattac tagaagtagt ttactttctg ttttaagtga
     4501 acaattctta gaaatggagg tgagtgttct tggctaattt ttttaacttt atactattac
     4561 aaaaagataa gattatagag cttttaatac aacatatgag tctaactgtt acatctattt
     4621 taatagctat aatagttggt gttcctcttg gaataattat ttcaagaatc tcatctctta
     4681 gaaagtttgt acttggattt gtaaacttag tccaagcagt tccgtctatg gcacttttgg
     4741 gtcttcttgt acctatactt ggtataggaa gtaaaccagc catatttatg gtagtggttt
     4801 attcattact accaattgta aaaaatacgt atataggtat aacaagtatt gacccagtag
     4861 tattagaatc agctaaaggt attggtctta caagaaatca gacattattt aaaatacaat
     4921 tccctttagc acttcctata ataatgggtg gagttagaat atctgcagtt acagctgttg
     4981 gtttgatgac tcttgcagca tttataggtg ctggtggttt aggatattta gtattttcag
     5041 gggttcaaac tgtaaataat aatatgattt tagcaggggc aatcccatca tgtataattg
     5101 cactgttagt tgattattta tttagtaaaa ttgaagttgc agttactcca aaaggattaa
     5161 atccaaaagc acctaagaag aattatatag cacttaaagt aatatctgta gtggttgttg
     5221 tttcaatgtt gttcgttgtt tttagttcta gtttttcaag taaaaaagat acaataacta
     5281 ttggttctaa agattataca gaacaactta tacttggtaa tgtttatgct gaattagtag
     5341 aaaaaaatac gaatcttaaa gttaagaaga atttaaatct aggtggttca tctgttgcgt
     5401 ttaatgcaat taaatctggt gagttagaca tgtatgttga ctatacaggt acacttttgg
     5461 taaatgtaat gaaacatgct ccgattaaag atgctgatga agcatataat gttgtaaaag
     5521 atactatgga aaaagaaaat cagttaactt tacttgaccc tcttggattt aataatactt
     5581 acacattagc tatgatgcct gaaactgctg aaaagtatgg aataaatact atttctgatt
     5641 tgactaaata tggtaaagaa tttacattct caccaacttt ggagtttgaa aatagagaag
     5701 atggtcttgt agggctttct agagattatg gtttaaaatt caaagatgta aaagcaatga
     5761 atggaagttt aagatataca gctttagata ataatgaatc tcaggttata gatgcatttt
     5821 taactgatgg tcttattaaa aagtttaatc ttaagatatt agaggacgat aaaaatttct
     5881 ttgtaccata ttatgcagca ccattagtaa gagaagatac tttgaagaaa tatcctgagt
     5941 tagaaaaagt tatgaatatg ctttcaggaa aaataaatga agaaactatg agagaactta
     6001 actatcaagt ggatgaactt ggtaaatcac cagaagaagt tgctcattca ttcttagtta
     6061 aagaaggctt agtataaaaa attaatagtt tatatcaaaa aatttaagag agtgtctcaa
     6121 aatgaacttt ttagttcatg aggcactctt ttttatatga aatcaaatat attttatcat
     6181 ttcaacaata aaaaagaaga ttatctttat tttgagacag tctcttattt ttatgtatac
     6241 caaaaccata cctacgattt ttatttataa ttaagtttca aatagaatga acaagtacaa
     6301 ccaattttga atataaatat agtaaatgtg cttatatacg attttattgt ctggttgatt
     6361 gatattgata gatagctttc tttattgaat cgaagggagg gggaatttgt tatggataac
     6421 tataagaaag tgaagcaagt attgtggata attttatttg ctaattttgc agtagcatta
     6481 ttaaaaatca ttattggtaa tcaaataaaa agctatagta tgacagcaga cggatttcat
     6541 tctttgtcag atggagcatc taatatagtg gggcttatag gtattttctt tgcatctaaa
     6601 ccaaaagata aaaatcatcc ttatggacat aaaaagtttg aaattataac cagtttattt
     6661 ataagtggta tgttgtttgt tatagcaatt aagataattc ttagtgcagt gttaagaata
     6721 gctaatccag ttgtacctgc tataactata gaaagtttaa ttgctttaat tattacactt
     6781 tttataaata tttttgtatg catgtatgaa tatagaattg ggactaaact aaatagttat
     6841 gttttgattt cggattcatt gcatacaaga agtgatatat ttgtgtctct aggtgtattg
     6901 gttactttag ttggtgttaa gttaggattt ccagtaataa tagaatctat agttcctatt
     6961 attatttctg cattcataat ttattcggct tatggaatat ttaggccatc tattggaatt
     7021 ttggtcgata gagtggctgt agatgaggat tatataaaag aaattgtgtt tgaatttaat
     7081 gaggtaaggg atgttcacaa tatcagaagt agaggtagca aaagttctat ttatattgac
     7141 atgcatgtaa tggtagaccc atttataagc gtagagcaat ctcatgattt aacacataaa
     7201 attgagaaac aaatacaaga agaaattaat gaaaatgctc aagtaatagt ccatattgaa
     7261 cctttttata gtttttaact tagctataaa atacaagtta taaaaaaata taatttataa
     7321 aaaaaatatt ataccataaa taatttataa aaattattta tggtataata ttttctataa
     7381 agaaattaag tcttaatata tctaaggagg aatttacaat gagatacgtt gaatatggaa
     7441 agacagggaa aatggtttca gtagttggct atggaggatt aaggtttgat ttagaaaaaa
     7501 gtaatcaaga aaatgctgat ttagtcaaat atgcgtatga aaaaggtatt aattactttg
     7561 atacagcacc agggtattgt gatgacagaa gtgaagatat atttggacta gcctttagac
     7621 aaatgataaa ggaaggtaag accgattttt atgtatcaac taaagaaaag ccaaaagcat
     7681 gtgatacatc agaaaaagct atagaggctg taaaaaaatc aatagaacga ttaggcgtat
     7741 ctaaaataaa tttttatcat gtttggaata ttagaaagat ggaacattac gaactagcaa
     7801 tgagacctgg aggtcaatat gaaggattat tgaaatgtaa agaagaagga ttgatagacc
     7861 atatagtatt ttcatctcat caaccaggag atgaggttat agatattcta agtgaaaata
     7921 agtttgaagg tgtaactatg ggaataaata tattaaactt tccatatagg ctaaaaggtg
     7981 ccaaatatgc tgttgataat ggatatggtg ttgtggcaat gaatccatta agtggtggaa
     8041 ctattccaaa gtataataaa gaactttctt ttctatcaaa agatggcgaa agtactatag
     8101 aatcagcttt aagattcaac ataggaattt cacaaataag tatatctttg atagggttta
     8161 ataaaaaaca agatatagat gatgcatgta aaattgcaga cgaaaataga atttattcag
     8221 atgaggatat acatgatatt gagactagat taaataagaa tatgaacgaa atatgtacag
     8281 gttgtggtta ttgtaaagta tgtccaaaag gtattaatac cccagcctat atgctttttt
     8341 ataatgaaaa gcagatgttt aaaaagagtg atgaagaaat gacaaagtta gtttatggct
     8401 taggacattg gaattacaca atgaatagta aggcaaaagc taaagaatgt atatcttgtg
     8461 gaaaatgtga agttgaatgt actcagcatt tacctataat agatagatta aaagaaatta
     8521 aaaaatggga agaagatgga gctaatagtg ttaaagtcta attaatttta tactatattt
     8581 gaaat
//
LOCUS       DAYAOX010000154         8558 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.155, whole genome shotgun sequence.
ACCESSION   DAYAOX010000154 DAYAOX010000000
VERSION     DAYAOX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.155"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            180..1079
                     /locus_tag="ACGI57_002738"
     CDS             180..1079
                     /locus_tag="ACGI57_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020397798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9717807.1"
                     /translation="MSIVLNMPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKN
                     NKINFEAVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLV
                     GNYIAERYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVN
                     QKLSEWTSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLF
                     RYGGDEFLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDI
                     ETLISKADSLMYENKLKNKSTLN"
     gene            1288..1437
                     /locus_tag="ACGI57_002739"
     CDS             1288..1437
                     /locus_tag="ACGI57_002739"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9717808.1"
                     /translation="MTTVAEGIETSFQKDFLQEINCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(1697..4264)
                     /locus_tag="ACGI57_002740"
     CDS             complement(1697..4264)
                     /locus_tag="ACGI57_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HFG9717809.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYMSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(4267..4872)
                     /locus_tag="ACGI57_002741"
     CDS             complement(4267..4872)
                     /locus_tag="ACGI57_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9717810.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLNGIMNDGGE"
     gene            complement(5246..6706)
                     /locus_tag="ACGI57_002742"
     CDS             complement(5246..6706)
                     /locus_tag="ACGI57_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HFG9717811.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRGNIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            7060..7638
                     /locus_tag="ACGI57_002743"
     CDS             7060..7638
                     /locus_tag="ACGI57_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9717812.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            7929..>8558
                     /locus_tag="ACGI57_002744"
     CDS             7929..>8558
                     /locus_tag="ACGI57_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9717813.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDI"
BASE COUNT         2824 a         1532 c         1231 g         2971 t
ORIGIN      
        1 taatttttat tatttgtact tatttaaagt ttcatctaat tataaattgg acaattttta
       61 tgttgatttt attcatacaa attaaagtaa cttataaaat ctccacaata aaaaacattt
      121 tttattcact aaactgctcc atcattggtt tagcaataaa tatatttttt aggtctttaa
      181 tgtcaatagt gttaaatatg ccagcatatt tatttgatag taattctatt gcaaatattt
      241 acagagtatt tcctatatca tttagcttta tagttacagg actatatttc agttataaaa
      301 ataataaaaa taataagatt aactttgagg cagttttaga ggacaaaaaa aacttaattt
      361 tcttattgag ccttatgttt ggaatgtact tgtatctatg tttgagtttg tcaatatact
      421 atactgttgg aaatgtattg attttaaaat tatatggaat caagtctgcc ataatagttt
      481 tggtaggtaa ctatattgca gaaagatata gctttagaat tactcaaata aaaggttatg
      541 atattaaaaa tcaaatagaa tatgtagaaa gtgtaaagac agaatatgag attaataaat
      601 tgaatgatat tgtatataca gatttactga caggtttcta caatagacct tttgtgaatc
      661 aaaaactatc agaatggaca tcaaatcact acaaatttac cttatgtttt actgatttaa
      721 atagcttaaa acctgtaaat gataaatttg gacaccaatt tggtgataaa tacatatcca
      781 ctactgctca aataataaaa aaacttataa atgtagaaaa ttccttacta tttagatatg
      841 gtggagatga atttttattc cttttacaga atacaagttg tgtagaagca gaaaagataa
      901 tgattaatat aaattatgaa ttatcaaaat taagtaatac tgacgagtat ccttatgctc
      961 tttccataag ctatggatta gttgagtggg atggaatatc cgatattgaa actttaatat
     1021 caaaggcaga ttctttgatg tatgaaaata agcttaaaaa taaatctact ttaaactagc
     1081 tagttaagat attctacaaa atatatgtta taagagagta ctaagaatat caaaatatat
     1141 agattttgca aatattgaat acagtttact aatgaatgca ctggatgtaa gtgttagtaa
     1201 acacctatta gatgaacact ttactgttgt ttgggctaat gaccgttatt atgaaatgtt
     1261 cagctatacc tagctaaaaa gttaaaaatg accactgtag ccgagggaat agagaccagt
     1321 tttcaaaaag atttcttgca agaaataaac tgtgatatgt tacaaggata cgtgttctct
     1381 agacctttac caataaaaga ctttgaaaaa cttatgtttc aaaactcctc caattaacaa
     1441 tataaaattg cttattataa gtgatattat tgtatcaaca aataaaggaa ttgtctcaaa
     1501 attgattttt tctaatcaaa tttttatttt gagacattct catttttttg cataagaaaa
     1561 tacccaccaa gctacggttt ttcttaccgc tgttcggtgg gtattagtag tattatgaaa
     1621 tctgaaaact ttaagtatta ctacttccgc taacgcaatc acaaaggtcg aaagcttcaa
     1681 agcgaaaata ggctttttac tcatcaaacg tcaccgtacc tgccaacccg tccaccgtta
     1741 tcatttgtcc gctcttaata gttcgtgtgg caatgtgtgt agccataacg gctgggatgc
     1801 catattcacg tgcgacaatg cttgaatggc taagaggacc accaatgtcc gttacaatcg
     1861 cacttgcaga tgaaaataat ggtgtccatg ctggagttgt ggtaactgca accaaaacac
     1921 ttccaggttg aaagctttca aaatctgctg gactattcag tacacaagct ggagccgtca
     1981 caacaccagt actggtacca atgcccgtca acacaatttt cccatctttc tgcttctgtt
     2041 gtgcttgtga tatagttttt ttgtttttct ctggcaactt ggatggtgac atataaccca
     2101 tgtatttttt aagttcagct tttcgaacag gtattatccc gttcatatca gatagagact
     2161 tgtttttatc caactgctct ataagtacct ccaattccga ttttgtgagc caataaatat
     2221 catctaaatg tgaaatagct cctcctcgta tgaatcgttc tgagatttcc tgaaacatac
     2281 aacgaatcag aggatgtccc attcccatca aataaatagc attttcacgc ataggagagg
     2341 tttcctgtgc ccaatggagc aacttaaaaa atagtttttt acgcaatcca cctatgtgtt
     2401 gtaaaatctc ttcttctgca tgttttctac gcttttcaaa cgtggtctgg cgtaaaaaag
     2461 gactttcccc ttttccttcc acaaatgttt ttacggattc aaaagttggt gtaagtgttt
     2521 cctgcggtgt ggaatgcgca aaatcaaact cataagcggt acgaccaaac tctttaaagt
     2581 acttattgat tcgatttttc cattctatcc atacttcctg tgaaactcca acaggcacaa
     2641 ctgaagacat gaaatcttct gctatcttta ttgcaggact gttttgcaga taaaggttga
     2701 tgctgttatt ttgctttacc cattctgaaa taccccacaa ctccttttcc gattgcagaa
     2761 caatcgtatc aaacccaagc aggaaaacgg aagtatccgt tataccagca cgtcgaactg
     2821 ctccttgaaa taattttgta aataatgttt cactgataga agcagcaggc aatgtaagct
     2881 gaatccttgt gaaataaata catgcggcat aaaatattgt ctgaataccc tccataattt
     2941 gatgggcgtt catagtatgc aggggctttt cttcccattg tttaatagca tcctcatatc
     3001 cttttcgtgc cgtttcccag cgtgcaacgc tgttcataag tgtcctacgt aaagagcgtg
     3061 gggaaaggga tttgatagca atcaaaagtg gctttgattt agcagaaaga taaacatatc
     3121 cattaataac cgtatatgcc ccgttttttg gtaataattt tttcgcactt ttaccaaaca
     3181 tatctatcca taaaagctct gatgcacggt ttacgatttc aagaccaagt gtggcgaaca
     3241 aaggtgtaac aggatttggc aaatgttctg ccaaacttcc ctttgtgtat acaacatcct
     3301 gttctggcaa tacccattct ggtggcagaa cagtaattgg acgagcctga acaatgtaca
     3361 atttatcttt ttccagtgcc cactctacat ccataggcat ttgataatac ttctcaattt
     3421 tctttccaag ctgtatcagt tgcattacct gattacgagt aagagcatat ttttttctca
     3481 acctttcagg aaccatggtt tcttctgttc cttctgaagt acgaaccgtc ataatctctt
     3541 tatttgccac ttcatacgat ataattcgtt cggagtcctt atccactacg attgtatcgg
     3601 gtgtaactaa gctagaaacc accgcttcac caagccccca tgcagcgtta ataatcattt
     3661 cgctacgtct accattgatt gggtttaacg taaacataat accagatgaa tcggaaaatg
     3721 caagcttttg tactacaaca gcaagtgcga caatctcgtg ctttatatta ttcttcacac
     3781 gataagcaat agcacgagct gtccataagg aagcccagca tctctttaca gagtcaataa
     3841 ctttatcttc accttgtatg ttaagataag tttcctgctg acctgcaaaa gaagcgtcag
     3901 gtaaatcttc agcagtcgca gacgaacgga cagccaccga tatgttccct aatccagcat
     3961 aagccatttt aattgcgtcc gacacttcct gtggcatttc tccattatgg aaaagctccc
     4021 ctattttctt agatacatct tcaagttggc tggtattatt ggaatcaata ccatctagca
     4081 gtttattaat tcgagactga atatggttcg tctcaacaaa aatctggtat gaagcagtcg
     4141 taacatgaaa accgtctggc acagggatgc ctgctgtcaa tagttttgat aaagacatcc
     4201 cttttccacc aacaatttca atggttgctt gtttatgttc cagtggcaag acataataaa
     4261 tcataatcat tcacctccat cattcataat tccgttcaga aatatattca ttgtttcatc
     4321 caacattttt tccgaactat ccaatggtcg tgacgtgtag ctgattgata acatggagtt
     4381 aattaacaaa cgtgtcacaa gagcggtatc cgttttgcga aagatgcctg tggtcacacc
     4441 attatcaatt accgatttta taatatcttg atatgcataa cgagcatctt taagtctctt
     4501 tcgatactct tcttctaaat aatcagattc tgtatttaac cacatcagta cagttctata
     4561 ctgctctgga aatctaagat gattaagcat gatttttcta aggcactgtt gtggtgaagg
     4621 ttcaccatca ataatcctat gaacttgttg gatagttttt tctatttctt tctcaactgc
     4681 atatacaaca atctcgtctt tcgttttaaa aaaatcgtat agacttgact ttcccatgtt
     4741 ggctaaaact gcaatttctc tcattgatgt tttttggaat ccattttcag ccatgagact
     4801 caaagcaaca gcaactattt tttctcggcg ttctgcttgt tgttcgggtg taagcacaat
     4861 tccttttggc atagtgcgat acctcctttt agcgaccact ggtcgtttct tttttattta
     4921 tcatacagca accaacggtc acttgtcaag gttaattttt ttcattgtat atggtactcg
     4981 agataaaaat gttcatttta gaaattactc tatttaagta gaaataggct tcatcattag
     5041 aaagcaagac tataaatttg agatatttat catgttcaaa tttcatcaat atcaaagttt
     5101 aaaatattaa taaaaaaaga attactaaga aataacatat aaagattcaa aaaaggagtt
     5161 gaacttaaaa ttcaattaca aaaaatcaaa tttaagacaa ctcctttatt ctttatctct
     5221 actattcttt atctctattt atttattaca actccctaac atttaccact actgccttag
     5281 gtctagtcat agcctcaatt gaatacttaa caccttgagt tcccatacca gatgccttta
     5341 cacctaaaaa tgggaagtgg tctggacctc tttcagtctt attatttatt tgaacagtac
     5401 caacttcaag tttatcagct atataaaatg ccttatcaat atcacttgta aatactgatg
     5461 attgaagccc atactctgat tgattagcaa tttcaattgc ttgatttata tctttaactc
     5521 ttataattgg aagtactgga ccaaatggtt cttcccaagc aattttcata tcaacattta
     5581 cattatctaa caatgttgga tagaataaat tattttttct aacattgcct gttattaact
     5641 ttgctccttt atgtaatgca tcatcaacca acccttgaac aaaatcagtt gctttattat
     5701 ctataagagg agtgataact acttcatcca ttggatttcc aattgtcaac ttctctacca
     5761 atggctttat ttttccgact aatttatccg ccacactctc ttgtactaaa attctcttta
     5821 ctgccgtaca tctttgtcct gaatatgaat acgctccaga tactatattc ttagctgcaa
     5881 aatctaagtc tgcatcttct aatactatag ccgcatcttt tccaccaagc tccaataata
     5941 atggtaccat tccagatatt ttagatatat gctgaccaac ttcagtacta ccagtaaagt
     6001 taataaaatt aattccttta tgcgttacaa cataatcccc tatctcacta cccctacctg
     6061 taatagtatt aagtactcca tctggtatcc cagcttttct cataatttct accacatgaa
     6121 gtgcgctaat agccccttgt gtagcaggtt ttaatacgac tgcatttcca ccaataagtg
     6181 ctggagcaat ctttgacatt gataaattta caggataatt aaaaggagat atagctaaaa
     6241 cagtacctaa tggaaccctt gaaacataag acatcttatt tcttgttcca ccagggaaat
     6301 tatctccact aatggcttct ccctccatat tttttcccac atcagctgtg tatcttaaaa
     6361 aatctgctgt tctctcaact tctgcccttg cagattttat gtctttggct atttcaagta
     6421 ccagtatatt agctatctca tctatatttt ctaacaataa atcagcagcc ttatgaaata
     6481 tattgcctct cttgtataca ggcatttcag cccatacttt tatactctct tttgtgtttt
     6541 ttataacctc atctacctca tgttttgtca tagattgtac tttcccaaca agagaattat
     6601 ctattggtga gtaaatttct attggctttc tcgaattaga ttctacccat tttccattta
     6661 ttaaattttt atatacattt tcttttgttt taagttcatt aaacatattt ttattcctct
     6721 ctctgatata actttgtgta tttaaattat agccatttat tcgttatgta aacatttctt
     6781 tttttcataa acgtttacct ataaaaaaat tttaagttaa cgtttttcta cattcttcta
     6841 ttttaataac atactcgctc caaatctata taggtcacac tttaatacta atatatcaat
     6901 ataccaatat accaatatac caatatacca atataccaat ataaatatta taaacataaa
     6961 tcacttgaca aatttataag tagacaatat tatatatcta tgactcaaaa tatttttaaa
     7021 gtcatatata tcatatatag acagaaagga ttgataaaaa tggctaaaag ttttacagaa
     7081 atagaaaaag acaatataag aaaaagacta atttcagaat gtgaaatcaa ctggtctaaa
     7141 tatggataca aaaaaactaa tattgatgaa ctttgttcaa aatcaggaat tgcaaagggt
     7201 tctttctata cattttttga ttcaaaagaa aagttatttt ttgaagtatt aaatggaatc
     7261 caaaacagac ttatatcatc agtagatgaa attttaagta acatgcctcc aaaagaaggc
     7321 tttgcaaaat ccttaaaaat gttatatcga ttatatgata aaaatccatt tttatattca
     7381 ccaaataatc ctgatttcat cagcctaata aataaactac ctcaagatat gtttttacaa
     7441 cttgagtcca ataacataca aagtttttac gacttaattg agagacacaa tctcaagttg
     7501 aagatagaaa aagaaaaagt ttttggtgtt tgtaatgctc tactatcaag tctgttggta
     7561 aaagaaaata ttgaatataa ctatattgaa gtgtttgact ttatggtgga taatcttgta
     7621 gaacacatac ttgaataaca atttaaaata atttttctaa gtattttttt atactaaata
     7681 ggattaatat gattgaatta atgcaaaaaa taaacaatat aatttataaa ataaatgata
     7741 aactaaatat cacacaaaac ttatagaccc tagagcaaac ccattccact ctactctaag
     7801 ccatctataa tagtttgtat atttctaata atattagata ttaaagtaag aaaaaaattc
     7861 cttataataa ttgctattat atcaattatt gtaaatgttt taataatctt agataggtag
     7921 gtacatatat ggtaaaatcc tcgaacactt ttaataaaaa cttcaacttg atgatattag
     7981 gtcaaataat ctcattgttt ggtgcatcaa tacttaaatt tgctttatct ttatacatac
     8041 ttgatataac tggtaaagca gaaatatttg ctactatact agcagtatct tcaatcccaa
     8101 ttatagtatt ctcccctatt ggtggagcta ttgcagatat attcaacaga agaaacttaa
     8161 tggttatttt tgatttttca agtagtctta cagtactaat cttagcagta ttcttattca
     8221 acaataatgg ttcaatatta cttgtaggaa ttattatgac catattatca gtcataagca
     8281 caatgtatca accaactgtt cagtcaagta cacccctttt agttgataat gagcatttaa
     8341 tgaatgcaaa tggtattgta gctggagttg catctcttac aaatattgca ggacctgtat
     8401 taggaggtgt cttgtatggt gtgattggaa ttaatgcaat tatagttata agttgtgtaa
     8461 gtttctttct atctgcaatt atggagatat ttatacagat tccttttatc aaacaagagc
     8521 aaaacagcaa cattataaaa actatattta ctgatata
//
LOCUS       DAYAOX010000155         8538 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.156, whole genome shotgun sequence.
ACCESSION   DAYAOX010000155 DAYAOX010000000
VERSION     DAYAOX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.156"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            96..1316
                     /locus_tag="ACGI57_002745"
     CDS             96..1316
                     /locus_tag="ACGI57_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9717814.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIEIKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1353..2702
                     /locus_tag="ACGI57_002746"
     CDS             1353..2702
                     /locus_tag="ACGI57_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9717815.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKFFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            2816..3378
                     /locus_tag="ACGI57_002747"
                     /pseudo
     CDS             2816..3378
                     /locus_tag="ACGI57_002747"
                     /inference="COORDINATES: protein motif:HMM:NF037635.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3486..5819)
                     /locus_tag="ACGI57_002748"
     CDS             complement(3486..5819)
                     /locus_tag="ACGI57_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717816.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSRLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(5816..6499)
                     /locus_tag="ACGI57_002749"
     CDS             complement(5816..6499)
                     /locus_tag="ACGI57_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717817.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            complement(6570..7811)
                     /locus_tag="ACGI57_002750"
     CDS             complement(6570..7811)
                     /locus_tag="ACGI57_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9717818.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            complement(7822..8508)
                     /locus_tag="ACGI57_002751"
     CDS             complement(7822..8508)
                     /locus_tag="ACGI57_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9717819.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKNLLTVR
                     RMGYKWNVIK"
BASE COUNT         2972 a         1320 c         1101 g         3145 t
ORIGIN      
        1 attcttataa aaaacaaagt tctccatcga aaattatggc ataataattg cttattatat
       61 taataaaaca ataaaaagca attggaggaa taaaaatgca acaggctttg aaattaaaat
      121 atcaaaccaa agaattagga aaagattttg taatagaaag ttgtaattct ataaaaccat
      181 ggcttataaa cataagaaga gaattacata aaattccaga attagctctt gaagaaaatc
      241 ttactaaaca gaaggtaatt tcttatttaa aagaaatcgg aattgattat atggaattta
      301 caaaacacaa tggaattatg gcatatattt taaaagaaag tgcagataaa acgatttgta
      361 taagagcaga tatggatgct cttcctattg aagaagaaaa caacatacct tataaatcaa
      421 tacattctgg aaaaatgcat gcttgtggcc atgatgcaca tacaaccatg ctacttggag
      481 catgtaaagt tttacattca ataaaagata aacttaatgt aaatgtaaaa ttcttatttc
      541 aaccagcaga agaaggtttt ggaggagcaa aattcttagt tgaagatgga tgtttagaaa
      601 atcctaaggt cgattatata ttcggacttc atgttatgcc acatatcgaa acaggattta
      661 tcgaaataaa atatgataca ttaaatgcta gtgtagacac tataaaaata agtatcaaag
      721 gtaaaagagc tcatggtgca tatcctgaaa atggtattga tgcaatagtt acagcttcac
      781 aaattgttac atcattacaa actataataa gtagaaactt agaaccaaat aatgctgctg
      841 ttttgactat aggtaaaata tatggtggtg atgcacacaa tgtaatatgt gaagatgtaa
      901 aactagaagg cactttaaga accttaaata gcaaaacaag aaactttatg attgataaaa
      961 tagctaaaat tgttggagac actgcatctg catttggttg tgttgggact ttacatgtaa
     1021 gtgatgaaaa ctatcctgct gttataaatg aaaaagaatt agtagataca gttatatcaa
     1081 atactaaaga attacttgga gaggaaaaat ttatattgag accaaacccg tctttaggtg
     1141 gtgaagactt ttctttttac acagaacatt gtaaaggagc attcttccat ttaggatgta
     1201 aaaatgagga aaagggctta atttcacctc ttcatacctc tagctttaat atagatgaag
     1261 attgtttacc aataggtgtt atgatgcatg taatgaatac tttgtatttt aattagtaca
     1321 gtaattaata aaagaatagg agataaatta atatggaaaa agctaagaaa ttaggtctat
     1381 ttagtatgat attattaggg attaatagca taataggttc aggtatattc ttactaccag
     1441 gtaaagttta taatctagca ggtcaaaaca gtatgtttat atacattttt gctacactct
     1501 tagttctatc aatactttta tgttttgcag aagttggaag catgtttgat aagaatggag
     1561 gagcatatct atactccaaa aaagcttttg gagattttat aggatttgaa gttggtacaa
     1621 tgtcttgggt tattagaatt atatcatggt ctactctagc agtaggattt gcaacagctt
     1681 taggttcatt ttggccagaa tctgccactg aatacaaagg ttatatagct gcaatactcg
     1741 taacactact ttcaataaat agcttatttg gaattaaaag cacaaagata atgaataatg
     1801 taataactat agctaaatta gtaccattaa tagtttttat aatagttgga atattcttta
     1861 taaaatttgt aaatattgtt ccttcaggaa atgtagttaa ctctagtatg ggtccagcca
     1921 ttatattagt attttacgca tttaccggct ttgaatcatt tattgtggca agtggagaaa
     1981 tggaaaatcc taaaaaaaat cttccagtcg cattaataac cacaatattt atatgtgcaa
     2041 taatttatat attaattcaa attgtctgta tgggtatact tggagataga ctatttgaaa
     2101 acagtattcc aattgcagat gcttcaagtg tatttttagg caattatggt aaatttttca
     2161 tatctgtagc aacattaata tctatatttg gtataaatat aggttcatca atagtaactc
     2221 ctaaatgtgg ttcttctcta gcagaagaag gttctcttcc tgcatttata ggaaaaacaa
     2281 ataaatatgg tgctccatat gtagcaataa taatatcttt aatttgttgt ataccacttg
     2341 tgctaactgg aagttttgaa caacttgcag taatgagtgt aattgcaaga tttgctcagt
     2401 acataccaac ttgtttatca gtaatagttt taagaaaaag aacagatgtt aaggcctctt
     2461 ttaagattcc atttggtcct gttataccaa tggtagctat tttaggtagc ttatggttgt
     2521 tacaacaagc ttgggcagaa gatatttcaa aacctataac tcaaaataga gttttaatag
     2581 gattaggggc aatgttgtta atagctcctc tatatatatt tatgaaaaaa aataaggaaa
     2641 tcgagcataa aaataaatca gaagattcta aattatatat ttcgagagaa aaaattcaat
     2701 aatattttac aaactattta aaaattattt ttctaatact atagaaataa ttagaaatgg
     2761 agatgttacg tatctaaaag tagttgataa cacggatatt ggtactttta tagatatggg
     2821 acttacaaaa gacatactag ttccttttaa agctaagact tatccattgt ttagagatga
     2881 aaaatattta ttctatatat acttggataa aagcggaaga atagctgcta ctacagacat
     2941 agattcatat ttagaaacag accatactta caatgtagga gatgttgtaa atggagttgt
     3001 atatggattc caaaccaaca actctgctat gatatgtgtt gacaataaat atgctggtgt
     3061 aatccttcat aatgaatact ttacagaatt aaaagctggg gatgttcttg aaaatttaca
     3121 tgttataaaa atatacgatg atggtaaatt aggactttct cctagaggaa acagaaaaga
     3181 tgaacttgat accttagaaa ataagatttt aagctattta gaaggttcag atggatatat
     3241 gagattcaat gataaatctg atcctaaaga tatttctatc ttatttaatt caagtaagaa
     3301 aattttaaac gtgctcttgg agtacttatg aagaaagact taatttatca agatgaagat
     3361 ggaacttatc taaagtaaat tatataacac aaaaatcgaa gtaaattatg aaagatattt
     3421 tctatttcat atttacttcg attttttatt taaattacta cctctattta aattactact
     3481 tatatttatt gtgcatttac tacctctatt atgtctaatt cacaaatctt tttaattggt
     3541 tttcttactg caagcaaagt tattattatc atagttacta caatgataaa cagtaaatca
     3601 aaaggtattt gccagtctat atggaatttt aatgttacta aggatacaaa tatatatcta
     3661 tgtaatatta atccaagtac actccccaat aaacagccac ttacagcata tgtacttgat
     3721 tctactatta ccatttttct taattgctta ttggacatgc ctattgcacg cataatacca
     3781 tagtaattta ttctacttgt aacgcttata ttcatactgt ttatgatatt aaacatactt
     3841 ataatcgcaa ttataaatac aaacccatat acaaaaactg ccatagtgta aaatgaattt
     3901 ttagcctcta agttaccttg tcgcttatct cgaacctcta aattatcatc tagaacattt
     3961 cttatatttt ggattaaatc ttcaggagca tcttcatcaa cttgagtatc aattattata
     4021 tagtctttta tatctgttat actagtaaaa gtattttcag atgttataat atttccaata
     4081 tatttgtctt taacatcaaa tgaaattgaa gaaagtatac ctgctacctc tatctctttc
     4141 ttagaatttc catttttaat atatatctta tctccaatat taaacccatg accttcttct
     4201 accaatccga tattttctga ctctaattta tcttcattga tttccccttt tactagatac
     4261 tttttggccc agtcaaattg atttttttca taagaaatta gctgagatgt tgcctcctta
     4321 ttattgtatg tagcctctac atccgtcatc attctactgt atactttttt tattcctttg
     4381 atattactta tttcattaat atcgtcttta gtaagaacag acttaaagtc agcgtcaaca
     4441 atagatatat gagcagcttc tggttttata ggtttaagtg cctgatacat aaagtctata
     4501 aatacactaa aacctaaaaa caatacaata ctaatagcaa atgaagaagt cataagtata
     4561 aaagattttt ttcttgaaaa ggcatgattt attcccatag aaacatcaat tttagaactt
     4621 cctgcgttag caattttact tgcttttcca ctagttaaaa tttctttgaa cattagatta
     4681 ccacgtatag cacttagtgg agacacccca gaagcttttt ttgctggaac aatagctgat
     4741 aatattactg ttaaaaatcc tactattatt ccaaacacaa taccaataaa gctaaccttt
     4801 aaaattggca tcgaaccaaa taactctgta ttcacatatt tgagaaatat agaggatata
     4861 atggttacaa tacatcctaa taacaatcct agtggaatag cctttacact tagaattaaa
     4921 ctttctaaaa caacaaattt tttgacttgg agttttgatg ctcccaagca acgtaatagt
     4981 ccaaaaaact ttattctctc tattacaccc atattaaagc tattatatat catgataata
     5041 cttgctatta acacaagtat aaaaagtcct cctgctgtta agtaaagtgg tttagaataa
     5101 tcagagtcac cttgacctat aatagaaagt aatttttcat ttttaccgat ttgtttatca
     5161 cttaaattga aggtattctt aatatcagaa gtagcttttc taatattaac cttgtcttta
     5221 aatgtaatat aataataatc atatgtttct gattttttga tagcttgtgt acctaatatt
     5281 gaaagcatta atccatgctt atctgaggct cttaatcttg ataatgatga atattcacct
     5341 actatcttat aatttttatt tctaccatct gctaatttta ttttgataaa atctcctact
     5401 tttagattaa agcttttaac tgcatctaca tctattaaag tctcattctc cacaacagga
     5461 taacgacctt ttattatttc tagacctaat tgttttgcta aatcttcatc taaaccttgt
     5521 atcaaaatct cctttgattt aaactctcca ttatcaatga cttgtcctct accatagtga
     5581 gctatatcta ctctactact tatttctgat atagcttctt cattaagatt tttaagtact
     5641 atgtgccaat ttccatcatc ttttataaat tgtacttttt gacattgtat agccatgtct
     5701 gctagtccaa aaatagcagt aactaaacat actgcaatga caatacataa aattgtagtt
     5761 ctattttttt tcttatgtac ctttgcatat tgtggtacta gttctaaata gcttctcatt
     5821 ctctattcac ccctaggtct ttaagcattc catcagaaac ctgtagtatc ctatctgctg
     5881 tagaagctat atttgtatta tgagttatca taataatagt ttggttatat ttttctgctg
     5941 tcacttttaa aagtgaaatt acatcactgc tatttttact gtcaagattt cctgttggct
     6001 catctgccaa tataattgat ggctttgtaa tcaatgctct tccaattgca accctttgtt
     6061 gctgaccacc agataattga tttggaagat gactttttct atctttaaga cctaaaattg
     6121 tcaatacttc atctagataa atagtgtctg gttttttgta atctagtagt aaaggaaata
     6181 ttatattttg ttccacagtt aattcgggta taagattata tgattgaaag ataaaaccta
     6241 tattacgacg tcgaaaaata gtctgttttt cttctttcat tgaacatatt tctttaccat
     6301 caacaaatat ttttcctgag gttggagtat caagacctcc taaaagattt aataatgtac
     6361 tcttacctga gccagattct ccaactacag taacaaactc tccctttgaa actgtaaatg
     6421 atacatcttt taatgcctct acttttgtat atccatttcc atatgtttta cataaattat
     6481 taaccacaag aatatccatt tgctctatct cctttctgat gattaatata agaataattc
     6541 atacacctta ctgtaagatg tatcctatct tacatattag taagctttaa aaaacttaga
     6601 gtaaatgtac ttccttgtaa aaaattactt tctacagtta tggttccatt gtgctgttct
     6661 atgatagatt taactagtgg caaccctaat ccaaggcctt gattactttt tgaaaactta
     6721 ctcctataaa atcttttaaa tatatgatgt atatcttcaa tatgaattcc atttccggta
     6781 tcactcactg aaataatagt tgttataggt gtttcaaccc attctatcat tattttatcg
     6841 ttctcttttg tattatctaa tgcatttttt ataaggttgc ttaaagcttc tgatatccat
     6901 tcataatcgc aaaacaagtt tgtattttct agacctctta tagagatact ctttccttct
     6961 ttttctgctc tagtttcaaa actatataaa attttagtaa tcaacttttt tacgttaaca
     7021 ctagctttgt taagaattat tgtctttgaa tctatctttg taatctttaa caagttttgt
     7081 atcaatgatt ctatacgctc taattcattt tgactcttct gcacaaaatt ctcaacaatt
     7141 tcactattat gactctcttc ctgcaatatt tgattataca tcatcaatgc tgtaagtgga
     7201 gtttttagtt gatgtgatat atctgaaatt gtatttttaa gaaattcttt attctgcttt
     7261 tctttcaata tatgtgtgtt tagagatgtt gacatagcat ctattgaacc aaataacttt
     7321 gataaactac cttcttcata agtatttaac cgtacattta tatttccatt cataaatgaa
     7381 tcaattttat aactagcttc ttctatcttg ttctgttgcc taaaaaaata cacatacaat
     7441 ataaaaataa tcatagtaaa gaacacaaat aaatacaaaa acatagtttt tatatattta
     7501 ccttgaaaca atttcacact tggcataaaa ctatcatctg taccaaagtc atatctagct
     7561 gtatttaaga tttcttttcc ttttaaagta tatttatctt cattagtagt aaatactgct
     7621 ggtatttttg atttatcaac acccatacta tttaagtatc cagctatatt ataatcatgt
     7681 tctaacagtg cacttttata atcattagtt atatttataa ctactatttg acctactact
     7741 ataaacatca ataaaacgga tataatcacc aaaaaaaata tttttatgtc tttattagaa
     7801 aataccttca tattaacacc ctcactttat aacattccat ttgtaaccca ttcttctaac
     7861 tgttaataga ttttttggat tttcaggatt atcttcaatc ttatttctta accttcttac
     7921 atatactgac agagtattat catctacaaa actaccattt ccatcccata acttatccaa
     7981 tatatttttt cttgtaagta caatattttt atttttcata agtaagcata ataatttata
     8041 ttctgctgta gtaagttcaa gctcaatttc atttttaaaa acacgatttt caagtaattt
     8101 aacagtaata ttatttgatt ttagttcagt tacattttca cttgtaaaat tataacgtct
     8161 aagtaaagct tttattcttg aaattaactc atttaattta aaaggttttg ttatgtaatc
     8221 atctccaccc atatcaagtc ccatcactac atttacttct tcatcagaag cagttaaaaa
     8281 tatgattggt acattagaag cttctcttac ccttttacaa atatcaaaac catcaccatc
     8341 aggtaaggat acatccaaaa gcagtaaatc ataatttttt tctttataaa tttgcaatgc
     8401 ttcttctact gttcttgcta catcaacact aaatccattc ttttgtatag aatattctaa
     8461 tccatatatc aaacttaaat catcttcaac caacaatata ctactcatct caatcacttc
     8521 cattttttta ttttttac
//
LOCUS       DAYAOX010000156         8495 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.157, whole genome shotgun sequence.
ACCESSION   DAYAOX010000156 DAYAOX010000000
VERSION     DAYAOX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.157"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            395..1093
                     /locus_tag="ACGI57_002752"
     CDS             395..1093
                     /locus_tag="ACGI57_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717820.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            1271..2161
                     /locus_tag="ACGI57_002753"
     CDS             1271..2161
                     /locus_tag="ACGI57_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717821.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2554..3510)
                     /locus_tag="ACGI57_002754"
     CDS             complement(2554..3510)
                     /locus_tag="ACGI57_002754"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9717822.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            4213..5208
                     /locus_tag="ACGI57_002755"
     CDS             4213..5208
                     /locus_tag="ACGI57_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9717823.1"
                     /translation="MSIIELNELTKAYRRFEKKAGFMGSVKSLFNRKYTEKTAILSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            5195..6049
                     /locus_tag="ACGI57_002756"
     CDS             5195..6049
                     /locus_tag="ACGI57_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717824.1"
                     /translation="MSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFDVVV
                     NMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSEEIC
                     NRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFTSNP
                     VQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFKMIIENIVEFITGTMIPLVLL
                     PETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYKRMR
                     TLFDGVGI"
     gene            6049..6840
                     /locus_tag="ACGI57_002757"
     CDS             6049..6840
                     /locus_tag="ACGI57_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HFG9717825.1"
                     /translation="MKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLMF
                     NSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYITK
                     PLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLLF
                     YDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMST
                     IPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            7125..>8495
                     /locus_tag="ACGI57_002758"
     CDS             7125..>8495
                     /locus_tag="ACGI57_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9717826.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYK"
BASE COUNT         3154 a          898 c         1333 g         3110 t
ORIGIN      
        1 gtttgatttt ttatatgatg atatatacat gtgataatgt atacatattt gttacgatag
       61 gctcctttat aaagaggtct tatgtaagat aagaaaaggc atatttatac taatacataa
      121 tagagtatat gcttatgtat gatagagatg tttcttaaga gcaaaactgc aatattgcta
      181 atttataaag taaaaatata tgaattataa aatacaaata atatatttat aaaaattatt
      241 gttttaattt ataataaaaa ttttttgttg aaaataatgc tcgaacctat aaaactatta
      301 actaatattt gtaaatgtat acttttgatg ataaaatata attaagttaa gttggttttt
      361 ataaacataa atttatttta aggaggtgaa ataaatgaaa atatctagtc aatatagaag
      421 tcaatattca tttagatatg aaagtaatat aaataataca agaataaatg aaagtatggt
      481 taagaaaaat gaaactgtag gaaaagacac ttatttatct aatattatga aacaaaagca
      541 agaacttaat gatagaatta gagatttaaa atatagacaa gaggtttata ctaaaaaaat
      601 aaatgacgca attaagaact tatgtaaatc agaaataaga gaaacaacta ataatttttc
      661 taatatagaa ataggtatta aaaatagcat tatagaagag aaaaataaaa gtacaatgtt
      721 agatgaaaat tcaacttatc taaatacaaa tgatgaaaaa gaatctttaa tcactaaaga
      781 gtctaatgaa aaaattgaag aagaaatatt aaatgatgaa aaattagaag agttagaaca
      841 aaaaaaggat tataaagagg attctaataa aaaagagaaa gtatctgagg acttatcttt
      901 agtaggtaaa actcgtgaag agcttgaaaa tatgcttaaa aattttataa atttaacaca
      961 agaagaaata atgaaacttg agtcgagaat agaaaagtta gataaaaatg ctgaagaata
     1021 caaacaaaat tcaaagacta atatatttga taaaacagat gaacaaaaaa aacatataaa
     1081 tgtactgatt taaacaatat gttttaaaaa aataagttta ttatataatg aaaatataaa
     1141 ggtacaattt caatacactt ctattaaacc aataatttat gaaccagact taatttcaat
     1201 ttactaataa ccatatattt atgagttaat agttttatgt tttaaaaaga attaaataag
     1261 ggggagtagt atgaagaaga atatattaac tactatgatt accatgatta ttatattgat
     1321 gatgagtatg acagtaatgg cagagccata tcaagaagat gaaacatcta gcttgaaaaa
     1381 acagagtgca aatgaacgta atcagactaa tataggcaca atggatggca gagtgcaaac
     1441 aaatgcaata agtaaaggtg ctaattatga ccctaaatat cctcttaaag gaatgttaac
     1501 acaacttggt cttaatacaa caggtaaaga gggatgggat ttactctatg gaggaaatcc
     1561 caatggagtt gagcttcgag cagatggtaa atattattat ataaataagg gtatggaagg
     1621 gctaggattt tatgcggcag cattgtcagg tggctcatat cagagaatag gctttggaaa
     1681 acttgaccct atagaagtaa tagctgatgg aaattggatt tcttacaaac aagctcaaga
     1741 tacacttaca gtaatcagga attttttaaa tagctttgac tggagaaatg catctgagat
     1801 ggaaagagct aatagagctg caaaattggt aaccgaagca aaatatgcag actctaaata
     1861 ttgtaacata gtctatggta atctggttga taaacgtggt gtatgtggaa gttttgcaag
     1921 tagctttcat ttattaacaa ggttaatggg aatggattca ctatctatat taaatccatc
     1981 attaaatcat gcttggaact atatacaaat tgatgggaaa tggtataggt ttgatgggtc
     2041 tgaaatatca gcttttggtg gatcattaga ctttgattac cgtaaattaa aggatgcaac
     2101 aagagagatg actacttact atgatgctaa ggcattgtct atattaggat ttaatcaata
     2161 aaaatagggg gaggcttctt atagagcctt tttttattga tttgaaattt aataaggaat
     2221 tttatatgaa aatttaatca ataaaagcaa aaaaggctct ttataaagcc tttctaagtg
     2281 tttttttaag taaaattttt tatatcaaca tcagttttaa tcaaaacata taacattttg
     2341 gacttacttt taaaaataaa attattaaaa tataatatta atcatgtttt tagtttatta
     2401 tattctataa tagtggtcat gttgactatt gtagttttat taagaatagt tggtttatat
     2461 aaatatatgc aaagatagct tgttgtttac tttacttatt agtatatagg acaagatact
     2521 ctaataaatt tgcctatttt aatatctttt gtactaaaac caactaaagt ttcctttatt
     2581 atttcgtttt gaataataca aagcataatc cgattgtttg tataaagact ggaaagtatc
     2641 agagttattt tctaatttag atatacctat agaacaagaa ttactctcta ggcataattc
     2701 taaaaccttc tttgccttat tttcaacatt ctcttttgat tgattactat attcaaaaac
     2761 agcaaattca tctccaccaa gtcttccaat aatagcatca tttacgaata ctttagtcaa
     2821 aatatttgaa aaatgtatta gtatttcatc tcctcgaaca tgaccaaaga tatcatttac
     2881 ctgtttaaaa ttatctaaat caaagatgta aaagtatcca tgtgaaacag ttttaagcat
     2941 ttcttttatg tgtacttcaa aaaaatgtct attgtatagt ccagtcataa ggtctttttc
     3001 tgattttagt ttaaattcat ttgccattgc tagtgcttct tgtgcttttt cagttattgt
     3061 tttagggtaa tactcatctt tcatttggtc aaagtttgga atagaatggt gaacatggat
     3121 aagtttaatc atatcatctg taactctgta taccatagta aatcgagtat ccatttctac
     3181 atagatatct ttttctaggt aatctttttc tattacccaa agagtaccat atactataaa
     3241 agcttcgtct gtgactttgg tacatgaata ccactcatct ataatctcaa agtctatttg
     3301 ctctgattct gtttggtctt tttcaagacc tataataaat tcttctaatg tttcgtaaaa
     3361 ttcatgtttt cctgttccaa ttaatgcaag gttgtcatct aataaggaaa taataaattt
     3421 aaggttttct tcttctggat ttaaaatata aatatgccaa attttttttg ataaatctct
     3481 taattgtttt tgtattgtat tattttccat agattacccc cttaagattt acagtttata
     3541 ttttaatgta ttataagttg aactcttatt acaatattat tataatatag aactaaagaa
     3601 attacatcct gtataggtaa aagtatatct tttacaataa aaatattaca gtaaaatgac
     3661 cagttttaat atacgtatta tatttttaat ttataaacga atattataaa tgaaatttaa
     3721 attcaacctt gaagaaataa gctaaatgta ttagtatatt aatccatgct tgaacataaa
     3781 taacctttat gatatgatat caataataat attatgagag gagtgccttg atgtcggagt
     3841 tatataatgg taaccaacaa ttaaatagtt ggagacacat atattctgta taatcagaaa
     3901 tttcaagtgt atttttaata catttaattt ttgtgtctta ttctgccttt atagataact
     3961 gacatatgac aacaaatagt atttaaaata tatattactt tgttaaatta tggtgtagta
     4021 ggctttaatc tgctacactt ttattaattt tgtatagtat tattttgata actattttga
     4081 aatagagagc attaattttt ctagtccaca ggagttttct gtttttgtgc tgttaaatat
     4141 aagcacagaa aaaggcagat atatacctgt ggactttttt tatataaatt atgaaatatg
     4201 gaggtagtta aaatgagtat aattgaactt aatgaactga ctaaagctta tagaagattt
     4261 gagaaaaagg caggatttat ggggagtgta aagtctttat ttaataggaa atatacagaa
     4321 aaaacagcta tattgtcatt tgatttaaag atagaagaag gagaatttgt tggattaata
     4381 ggtcaaaatg gagctggtaa aactactctt attaagatgt taactggtat tattgcaccg
     4441 tcttctggag aaatatctgt aatgggttat taccctaaca aattagaaaa taattttaaa
     4501 aaacaatatg ctgttgttat gggccaaaaa agtcaactat tctttgaatt gacaccagca
     4561 gatacgtttc ttttatttaa agaattatat gatatatcta atgaagaata taaaaaaaac
     4621 ttggattact ttatcgaatt atttgaggta tctgactatt taaatgttca agttaggacg
     4681 ctttctttag gagaaagaat gaaaatggaa ctaatagtag cccttctaca taatccaaaa
     4741 atattatttt tggatgaacc aactataggc ttagatgcta tagcccaaaa acaaattagg
     4801 tatttcctaa aagaagtaaa tgaaacaaaa ggaactacta ttatacttac ttctcattac
     4861 atggaagata ttaagagtct ctgtagtaga agtattgttg tagaaaatag ccgtaaaata
     4921 tatgatggtt ctacagagaa actatttaat cattttcaaa aatataaaaa aataacagtt
     4981 agtttttcca atgaaactaa cattgtattt ccaaaagatt gtaaagttgt tgaacaaaca
     5041 aatcataaga tatcagtaat tgtgccaaaa gaaagaggaa atgaaacgat taaatggatt
     5101 tttgataaat atgatgttaa agatgtaatg attgaagaag aagatatagg tagtattgta
     5161 gagagaatat ataagaaagg aagtgatgag tattatgagc aactataatg aagcaagttc
     5221 aaatgattca atgttttata aatttaaaca gagtattaga aggtattatg aagtagccaa
     5281 aattaacttt aaagtgcaac ttatctggag atttgatgtg gtggttaata tggtgtttac
     5341 gattgtaaaa attttatttg cttatatttt atggaaagca atttttggtg aaaataaaat
     5401 agtctctggt tttacattta actctatgtt gtcttactat ataataagct catttttagc
     5461 aggtattgat atgtcaaaga gtaccagtga agagatatgt aatcgtgtaa aattgggcac
     5521 cttttcaaag tacatgatta ttccaattaa tgttcaaaat tattttattg ctaaaaattt
     5581 tggaatgaca tgtttttatt ttgtatttaa tatgatttct gcatttgtat ggatttttat
     5641 atttaaaata gattttgcct ttacatcaaa tccagttcaa atattaattt caattttgat
     5701 gatttttcta gggtttattt ttatgatgaa actccacttc tttattggga ttttgtcatt
     5761 taaatttctt gagattagtt tttttaaaat gataattgaa aatattgttg aatttattac
     5821 aggaacaatg ataccacttg tactcttacc agaaactata gtaacagtta tgaaggtatt
     5881 tccattttat tatacaacat atttgccatc tatgttacta ataggtagaa acagcaatga
     5941 ggcaatacaa ggattaatta ttctagcatt atgggtttta tttttttctg tagtaaataa
     6001 agttatgtat aaaagaatga gaacattatt tgatggggtg ggtatatgat gaaaaaaaat
     6061 ttgaaaataa tatttatgtt attaaaaatg aaattatcaa agatgatggt atttagattc
     6121 gatttttttg gagcattttt tgtagatagt tcattattta tattacaact tctaatgttt
     6181 aatagtatat attctcatgt agactcaata gggggatggc aacaaggaga gatgttaata
     6241 tttataggaa ctttctcatt aataaatgcg ataaatatga cgattttctt ttttggtatt
     6301 tatgatattc cacgaaaaat ccaagaagga gaattagact actatattac aaaaccatta
     6361 gacccactat ttaggttaac atttgaaaat attaatattg gttcatcacc attaattcta
     6421 gcaagtatac ttattgtttt atatggaata tctgagttaa actcagagat aactttagtt
     6481 atgataataa tatatatttt catggtgtta ttgatgacat tactttttta tgatgtatgt
     6541 atcatactta gaacagtttc attttttgtt atatcatctt ctgcgataat gagattagaa
     6601 gataatttat tgccaatgaa tatgaaaatt cctggtgtaa tatatgaagg tgtttttaaa
     6661 gtattattct atttaatctt accatatgga attatgtcga caattccgac tcaaattttg
     6721 gctgggacaa taacaattaa tggattaata tactcaattt taattgtatt tttgtttaca
     6781 ttgtttatgt ggagattttg gaagttaggt atgagacatt ataaaagtgc aagtagttaa
     6841 taaattagta aaaatagaca atgtataact atatataaat acgtttcata tgacactaaa
     6901 ctatgtgaac aagtatttgc gctaaaggca ctgaaagcta taaaattatt aaagtaagga
     6961 tttaaaagtt tctttagtag aaatataatt gtgtaaaaaa gcaagattaa ttaaaactat
     7021 atccaaataa ttagatataa ttcttatgca gaaaggaggt ctgtaaatgt ctaattgttt
     7081 ggaatatatt ttaaaaataa ttaatgtaca gaaaaataaa caaaatgagt gatgtatatt
     7141 attcagaaaa tcatgaacaa gataatcttt atgagcaaga taatttactg gcacaaagat
     7201 ttaatttatt tactctattg aagtttgcat ttcctactat ttttatgatg atttttatgg
     7261 ggttgtatac aatcgtagat actatatttg tatcaagatt tattaataca aatgctctat
     7321 cagcaattaa tattgtatgt ccaattatta atcttatagt tggaattgga actatgcttg
     7381 ccactggagg aagtgctatt gtagcacgta agatgggaaa tggtgatatt gagggagcaa
     7441 ataaagcttt ttctttaatc gtattatcag gagttgtaat tggggtaata attactggta
     7501 taggtattgt atttatggat tatatcatta tatggttagg agcaagtgaa gttttaatta
     7561 aatattgtcg ttcatatctt tttattatct tggtttttgc ttctgcaaat atactacaag
     7621 tattatttca atgtttattt gtaacagctg gaaagcctca atttggatta tggctagtgt
     7681 taggtgctgg gtgtgctaat ataattcttg actatgtgtt cattgttctg ttacaaatgg
     7741 gtattagtgg agcagcactt gcaactggag taggatacct aattcctacc gtttcaggta
     7801 tggttttctt ttcaaaaaat aaagggttac ttaaattttg tatacctaga ctagataaaa
     7861 gaatattatt tgaagcttgt tttaatggtt catctgaaat ggtgggtcaa ctatcaacag
     7921 cagttactac ttttttattt aatgctatga tgatgaaatt aattggtgaa gatggggttg
     7981 cagctattac aataattatt tattctcaat ttcttttaac ttcgctttat ataggtttct
     8041 caatgggtgt tgcaccaata ataagttata actatggaag tgaaaataat acgcagttaa
     8101 atcatgtttt taagatttgt atattattta tttcagtagc atctttattg gtatttatct
     8161 tttctatatc atgttcacca atcattgtag agattttttc tcaaagagga agccgagttt
     8221 atgaaattgc aaggtctgga ttttatatat tttcttttag ttttattttt tgtgggtata
     8281 atatttttac ttcgtctatg tttacagcat tatcaaatgg aaaggtgtct gcaatattat
     8341 cttttctacg tacatttgga tttataacta taggtttatt gatattgcca aatatattaa
     8401 atataaatgg gatatggtta gcagtaccaa gtgctgagat tttaacttta tttttaacca
     8461 ttttttgtat atgtaagtat aagaatagat ataaa
//
LOCUS       DAYAOX010000157         8427 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.158, whole genome shotgun sequence.
ACCESSION   DAYAOX010000157 DAYAOX010000000
VERSION     DAYAOX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.158"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(75..545)
                     /locus_tag="ACGI57_002759"
     CDS             complement(75..545)
                     /locus_tag="ACGI57_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9717827.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
     gene            complement(1415..1804)
                     /locus_tag="ACGI57_002760"
     CDS             complement(1415..1804)
                     /locus_tag="ACGI57_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9717828.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(3565..4971)
                     /locus_tag="ACGI57_002761"
     CDS             complement(3565..4971)
                     /locus_tag="ACGI57_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9717829.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(5016..5723)
                     /locus_tag="ACGI57_002762"
     CDS             complement(5016..5723)
                     /locus_tag="ACGI57_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9717830.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            5885..6808
                     /locus_tag="ACGI57_002763"
     CDS             5885..6808
                     /locus_tag="ACGI57_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717831.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            6808..7569
                     /locus_tag="ACGI57_002764"
     CDS             6808..7569
                     /locus_tag="ACGI57_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717832.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            7581..8318
                     /locus_tag="ACGI57_002765"
     CDS             7581..8318
                     /locus_tag="ACGI57_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717833.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         2813 a         1382 c          867 g         3365 t
ORIGIN      
        1 atactagtcc tatatattat cttgaattag ttctattaca attaatttct actaaataat
       61 tataatttaa acaatcattc cttactattt tcacactgct ttacaaattt aattggatgt
      121 tgaattattt tagaagtttc ttctacagag tatccctcag ataaatatct tctagctata
      181 acttccatag actctcctat ctcaataata tgcttatccg ggtcataaat acgcactact
      241 ctttgttgcc attcatgttt tagtggctta tggacaaact ctacatcttt ataataattg
      301 agtttttcaa taaattcgtc aaaatcatca acctcaaaat aaagttccat attatttgat
      361 ttttgtataa ttgtatctgg attaatccct gtaagccatg cgaagtcttc ttgtatagca
      421 aatcctcctt taaatgttac atttttgcct aagtccaatt caatttcttg ctcaaataat
      481 tctttataaa atttttttga tacttcaata tccttcactg ctaataatga aacctgatat
      541 ttcataaatt ttcctcctaa ttttttattt ctattttttc agtatcttct attaacattt
      601 ttttctctat ctcttcagta ccttttatta atcctttatt tttacttttt tagcatcttc
      661 tattaacact tttgtttcta cctattcagt atcttatatc aatcttttat tttttcaata
      721 tcttctatta acacttttat ctctatctat tcagtacttt ctatcaatct tttgttttta
      781 tgttttcaat atcttctatt aacacttttg tttctacctc ttcagtatct tatatcaatc
      841 ttttattttt tcaatatctt ctattaacac ttttatctct atctattcag tactttctat
      901 caatcttttg tttttatttt ttcaatatct tctattaata cttttgtttc tacctcttca
      961 gtatcttata tcaatctttt attttttcaa tatcttctgt taacactttt atctctatct
     1021 ctttaatatc ttatattaat cctttatttt tattttttca atatcatcat taattcctat
     1081 ttattttatc atcttaaata cgaaaacact gtgtcatatt tgcaaaatat atattatcat
     1141 ctatattaaa gcttcacaat ttatatttaa aaattctctt taattacact acctctattt
     1201 aaaaagctac cttaattata cttataagta aaggtagctt tttaaattta attaaataga
     1261 atatccacta ttaatttata tttaatttat ttagattcac attatagtat cataatccac
     1321 ttataatcat ttgtcatatt acttaagtag actaatatat tacataaata cacttacata
     1381 cactttatat tgatgctata agaaatacgt ttgcttatac gtctttctcc tcttctttta
     1441 gattttttat ccactcttca ataagtaaaa tatcatcttt gctcaaatta atattttcat
     1501 gtaatgacaa caataaactt tttatagaag aatcatgaat atttctaaaa aaatctcttg
     1561 tctcaactcc tatgtattcc ttttctttaa ttaaaacttc ataatgcgta tatttatcta
     1621 tcttttgaga atttagaaat ctttttacta ctaatctaga taaaagagtc aaagttgtcg
     1681 tttgtttcca ttgatatttt tgctccattc ctaaaacaat atctctagat gttactttgg
     1741 aatctgattt ccatataaat ttcattatct ttaattctga ttgtggcaac ttttttatag
     1801 tcatgataaa accttgctac tcagaatata gacaagcaat atccataata aaatttttcg
     1861 attaaataca tcttatacgg atttatataa gctcagactt ataagatatt tttcaatctt
     1921 aatcctaaat ttagctattt gtacacggta aatatatata aataatgtca aaattctctt
     1981 gtattatatt gcttaatttt atgaaatatt ttgtactcag ctatctattt tggatacttg
     2041 tccagtagct ctcctttctt ttaaactctt tgattttaaa tatttgtatt tcagtaaatc
     2101 ctacttccta aattttttat ttactacaac ttattactga acttcctact tcctaaactt
     2161 tttatttact acaacttatt actgaacttc tcacttacta aatttcttac ttactataaa
     2221 ttataactaa acttctcact tactaaattt cttacttact ataaattact actaaacttc
     2281 ctactttcta aatttcttac ttgctacaaa ttattactaa acttcctctc cctaaatttc
     2341 ttacttgcta taaattatta ctaaacttcc tactttctaa atttcttact tactataaat
     2401 tattactaaa cttcctactc cctaattttc ttacttacta taaattatta ctaaatttcc
     2461 tactttctaa atttcttact tactacaaat tattactaaa cttcctactc cctaaatttc
     2521 ttacttgcta taaattatta ctaaacttcc tactccctaa gtttcttact tgctacaaat
     2581 tattactaaa cttcctactt cctaaatttc ttacttgcta taaattatta ctactaaact
     2641 tcccacttac taaatttctt acttactact acttataact aaacttccca cttcctaaat
     2701 ttcttactta ctaccactta taactaaact tcccacttcc taaatttctt acttactacc
     2761 acttataact aaacttccca cttcctaaat ttcttactta ctaccactta tcactaaact
     2821 tcctactttc taaatttctt gcttcttaag aataatgtta attttaattt taagattata
     2881 gtttttacct tgtttaattg aaacattcta tataattata tccttctatt ttagagtttt
     2941 tttatttata tattacaaag tttaaatttt ttcattaatt atattataaa ttttccatat
     3001 atttcaacat aaatacatat atatatatgt tatttctaaa aatactacac aaaaactcta
     3061 ctttagtaga actcaatggc gcaaaatact tcaatttaaa acatttatcg tactttaaat
     3121 aaataactat aataaaatat acttacaata gttacataga agctattata actacacaga
     3181 acagcttata attaaaactt ttttaggatg tcattatatt ttttcttaaa attaaaaaac
     3241 actataccat aattctattt tagtagaatt tttttgtcaa taataaaatt aaatattaac
     3301 taaaaaataa aaaaaatgga attatctaaa gaatttaata accttacaat aattccaaaa
     3361 tattatttct atgaaaattt tgataccatg agtaactcag ccataagcgc ataaagcaca
     3421 agttactctt tactttaata aaacttgaca agaaaagaat ttatccttat tctactctaa
     3481 tagagttaat ttgtcaataa agaataaaat tattttcaaa atattttcat tcttcattct
     3541 tatctgttat tattttataa aatatcactt aattagaggt aattttagta ttgtttttag
     3601 tccctgacta tcaatgctcg taatatctaa tttacctcta tgtgcattaa taatgccaga
     3661 tactatcaaa atacctagac catgattttg acctttggca tatgcctcac ttagtccatt
     3721 ttggctcttt aataaactat taattctttc ttgactcata ccttttccat catcttcaac
     3781 aattatatag caaaaatcta aatcagactt agaggtaacc cttatattac atccttgttg
     3841 gttatgagtt atactatttt gtattaaatt actaatagcc ctttttaata attttttatc
     3901 tgcatctata ataatatctt catttagtat atcaagttca acgtaaaagt cattatccaa
     3961 attattattt ataaactcag aaataagctc tctcaaaaac gcagaaactc taatcttatc
     4021 acaattcaat gtttgcatat tatattcaag tttagacact aaatttaggt catttactaa
     4081 ttctctgatt ttaatccctt gattgcatat aatagacact tgctcttgtt ctctatttga
     4141 aaggctactg ctttcttcaa gttctccagc atatccaatt atcattgaaa gtggagttct
     4201 tatatcatga gatattcctg caatccagtt agacctagct tcctctttct tcctaagttt
     4261 ttcatctttc atttgcaact ctttagatac agtattaata gattttgaaa tatcacttag
     4321 tattcctttt tcttgtaaaa gtactggttc tccttttgac ataagcttta tgccagtaat
     4381 aattggtttt atagatttca ccatttttaa acctatgaat agagctatta aaaaagtaac
     4441 tattgcatta accagaaaca tacctattat taacatagga aattgttcta tttccttaac
     4501 cgtataagta aggttaaact tagtatactt gtcttttgga taacctgtta ctaatattcc
     4561 atttggatgt tcccatacat acacaggata gtcctttaaa aagaaccttg aaaactttgc
     4621 tacatctgtc aaagtataat gacttggaat ttcttttggt aaatcaaatc cccatattct
     4681 atctccattg ttatcaatta acattgccca aactttattt ttatgtagta cttccttact
     4741 agaatcatct aaaataaact ttccatctct taaaatcaaa ttatcttcaa tcataatcaa
     4801 gtttgatttt atttctctac tatcacttgt atctaaatta tcagagtatt tagatacaaa
     4861 taacatatat gaaaacagaa gaaaacctgc tatatttaat acaataaaaa gtaatgaaat
     4921 caagatactt gaacctataa atcgcctcaa tatgtacttt acactatcca aaattaaatc
     4981 actcctcttg atttctttta ttctaatatc taaaattaat taatcatcaa cttatatcca
     5041 agacctctta ctgtaataag atgtttaggt gtagatggag ttttttctat cttctctcta
     5101 acccttctaa catgaaccat aagggtattt tcatatccat acatatcatc tccccaaatg
     5161 gcttgacata gtgaatcatt agtaacaatt ctccctttat tttcatacaa ctttaaaagc
     5221 aacgaatgct ctttagctgt taaagaaatc tcttgcccat ctttctcaac acatgcacta
     5281 tttaaattca caacaacaga atctcccagt ttaaatactg gcaactcctc ttcttctata
     5341 ggcacatata cacgatttaa tatacccaca agccttaaag taagttcctt tggtaaaaat
     5401 ggctttacta tataatcgtc agcccctaag cccaaaccaa tcaatctatc ttcatcttca
     5461 cctcttgcag agagaaaaat cactggtatt ttagatgtct ctctaatttt tctcataata
     5521 gaaaaaccat ctccatctgg caatacaaca tctaaaattg cacaatctgg ctttacattt
     5581 ctaaataccc taacagcttc ctcataactt gatgctaaaa atactctact aaatccctct
     5641 tttctcagga tttcatctat catttttaat aattccttgt ggtcatcaac cacaagtatt
     5701 tttttatctt ttattgactc cataatcatc accctccttt agtataccat atacccattt
     5761 tcacaacttt gcctaaatag acttttaagg ttattgtaag gtaaatttca gctaattgaa
     5821 atgtaaccaa gttattatac tgatataagt taaaagaatc aataagcaca gattaaaaaa
     5881 ggggatgaaa catatggaaa tgataattag aacacaaaga ctttcaaagg aatacaataa
     5941 aatatttaga gtaaaggata tagacctaag agttccacaa ggagctgtat atggtttttt
     6001 gggtcctaat ggagctggaa aatctaccac tcttaaaatg cttcttggac ttgctaaacc
     6061 cacacaagga aatattaata ttttaggtaa agagttaaat gaaaaaaata gaatatcaat
     6121 tttaaaagat ataggttctt tgatagaatc tccatcatac tatggtcatc ttacagggct
     6181 tgaaaacatg accataatgc aaagactctt agatttacct aaaaaaaatg taaatgaagc
     6241 attaaaaatt gtaaggttag aaaatcaaaa aaataaaaaa gttagtcaat attctttagg
     6301 tatgaagcaa cgtcttggca ttgctatggc aataatgaag tttcccaaat tattaatact
     6361 tgatgaacct acaaatggac tcgacccagc agggattgag gaaattagag aattaataaa
     6421 atctctacca gaaaaatacg ggatgactgt tctaatctct agccatcttt taagtgaaat
     6481 tgaccaaatt gccacatcaa taggaataat aaatcatggc gaactggttt ttcaagatag
     6541 cataaaagaa cttcataata gaggtcaaag tcagattgct atcaaaacta agaatgttgt
     6601 atctgcacaa aaactattaa atactaaagg atatatgtca cttatacaag atgggtactt
     6661 gactattgaa aatttaaaag actcagatgt cgctaaaata aatggtatgc ttgtccattc
     6721 aaatatagat gtatttagaa tagaagaaca taaaaaaagc ttggaagata tattcttaga
     6781 acttactggt aaggcggtga ctttataatg aaataccttg gtctagaatt ttataaactt
     6841 aaaagaaaga agattattct gatgaccttt ttatttgcag ctgttgaaat aatgtggtgt
     6901 atcgtaaatt caaatagagc tattacacgt aatccagata tgttaggtgg atttgaatat
     6961 tcctatatgc ttatgagttt tgcaagttta aatggtctat tctttcctat cttaatatca
     7021 ataattacat cacgtataag cgatatagaa cataaaggag atacatggaa gcttttaaaa
     7081 tcttctgtga catctttaaa tagtatttat ttatctaaat ttttatgttc tgctattttg
     7141 gtatctatac ctgtattgat gcaagtgtta tctattgtgt tgtttggaca ttttagaggg
     7201 gttattgagt caatttctgt atctttactt ttaaaataca cattaggaac aattcttgta
     7261 agtattgcta tcattgcact acacctatgg attgcaactg taattccaaa tcaaatgatt
     7321 gcaattacat ttggcatgat tggtagtttt ataggtttga catcaggttt gttttatcaa
     7381 ggtattcgta gattgtttat atggtcttat tacctagagt tatcaccttt aagttatgct
     7441 tatgataatg ttttaggttc atctgtctat agaattgata tgaatttttc tattacgatt
     7501 ttagttttct taattggaat gctaatttat tatattggaa gaaatcattt attaaaaaaa
     7561 gaagtatagg agggatttaa atgttaagga agtcagtttt gggagaaatt ctaaagttaa
     7621 aaaattcttt tatatggtat gtattattag ctttgccact tataagtgtg ttgattggtt
     7681 ctggtaattt ttatctaaat caaggcatac tcaaaaaaga atggtacagc ttatggactc
     7741 aagttagtct tttttatggg gaatttttct ttcctatctt aatcgcaatc ttttgtgcat
     7801 atatttgtag gcttgaacat atgaatcata attggaataa tgtaatgaca ctgccagtta
     7861 atttaaaaaa tatcttttta tctaaactta cagtagtttc aatacttaca gctattacac
     7921 aagttttctt tgttatcttt tatatagttg caggaaatat gtttggtttt tctggtagtg
     7981 tacctaaaga attgactggt tggattgcta aaggttggat tatttctatt tcaatagctt
     8041 ctatacaatt atacatgtct ataaagatta gaagttttgc aactcctatt ggtattagtc
     8101 tttgcctttc tctatttggt atgggattaa tagtagcaaa acctgctgtt ggtatatttt
     8161 tcccttattc tatgttaggt agtgctatgg gggttataaa gcaatctgct ttaaatgtta
     8221 atgacactat agttacaata tgtgttgcat ttattactac tatagtattt tctgaaattg
     8281 ccaatagaaa ttttagaaag aatgatgtta tttcttaatg gtaaatgcta gatatttttg
     8341 ggtttgaaat ttatattctt tttagttgaa atctatatcc attttattta aaaatatata
     8401 tcttttattt gaattttata tgtttta
//
LOCUS       DAYAOX010000158         8379 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.159, whole genome shotgun sequence.
ACCESSION   DAYAOX010000158 DAYAOX010000000
VERSION     DAYAOX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.159"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            220..1044
                     /locus_tag="ACGI57_002766"
     CDS             220..1044
                     /locus_tag="ACGI57_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFG9717834.1"
                     /translation="MNWIFSLTPALLGITNTILGEIIFYLAYTSPAIVGLFFISTIYP
                     QSAKQDFIYRYLSFKEIGIKWILLTILFFALTFITSLIIGNYFNVKTSDIDWIHIAVF
                     RPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVRFGFIGSSIILGLVCGIWYLGSYF
                     TPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVYINTSRSILSAGLVHMMYYFFNIQ
                     LLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFKQKVNSEIEQIKSDDFEFELDW"
     gene            1222..2046
                     /locus_tag="ACGI57_002767"
     CDS             1222..2046
                     /locus_tag="ACGI57_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9717835.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2172..2753
                     /locus_tag="ACGI57_002768"
     CDS             2172..2753
                     /locus_tag="ACGI57_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9717836.1"
                     /translation="MKADSTKEQLINATIQLLSSHRDVSSITAREIVAKANVNLAMIN
                     YYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFDFIGEHSQFI
                     KISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSFIQLLFLRSN
                     AFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            2857..3033
                     /locus_tag="ACGI57_002769"
     CDS             2857..3033
                     /locus_tag="ACGI57_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717837.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            4229..4981
                     /locus_tag="ACGI57_002770"
     CDS             4229..4981
                     /locus_tag="ACGI57_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717838.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            4971..7082
                     /locus_tag="ACGI57_002771"
     CDS             4971..7082
                     /locus_tag="ACGI57_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717839.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            7466..8176
                     /locus_tag="ACGI57_002772"
     CDS             7466..8176
                     /locus_tag="ACGI57_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9717840.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT         3168 a         1069 c         1113 g         3029 t
ORIGIN      
        1 tttagtgtaa aaataattaa tcaaaatata aaaaatagga tattattata ggcaataact
       61 aacatcaagc gataacttaa tacatataaa aataaataaa caattaataa aaattaaaac
      121 ttcattataa aacggaagga tagtgaaaaa atgcaactat ataacacccc ctttaataca
      181 aacaaacact taatctattt ctttacattt actctagtat tgaactggat attcagtctt
      241 acacctgcgt tgctcggaat tacaaataca atacttggag aaatcatttt ttatcttgca
      301 tatacatcac cagctattgt aggtctattt tttatttcta caatctatcc acaaagtgca
      361 aaacaagatt ttatttatag atacctaagc tttaaagaaa tcggaataaa atggatacta
      421 ttaacaatat tattttttgc tttaactttt attacttcgt taataatcgg aaattacttt
      481 aatgtaaaaa cttctgatat agattggata cacatagcag tgtttagacc ttctaaaata
      541 gcttatatgc tattagcaag ttttatattg agtatactta gtcaagaagc tggatggaga
      601 ggatatgcca ttgacaaatt attagttaga tttggattta taggctcttc aattatttta
      661 gggcttgttt gtggaatctg gtatctaggg tcgtatttta cacctaatca aataccatat
      721 aatttggcac aatactccct atttgatgct tttttattta taccaaacat tgtactttta
      781 aactttataa ttaattttgt atatatcaat acaagtagaa gcattttatc agctggatta
      841 gttcatatga tgtactattt tttcaacatt caattacttc tacactaccc tattaaatta
      901 tgtgtaatag ctcaatatgt acaaataata tttggtttaa tatttcttgt atatatgatt
      961 tcttctaata aatttaaaca gaaggtaaat tctgaaattg aacagataaa gtcggatgat
     1021 tttgaatttg agttagactg gtaattttaa tatgaattat aaattaagat aatctatgat
     1081 ttgatacgat tactacttaa atataattta ctctctataa tttattgcaa catctggaaa
     1141 ataattgtat ttccacaaac gcttgaagta tataaaaaca aaatatatac tgattataag
     1201 tgaaaaggag gtcatatttt catgtttaaa ataggagagg tttcaaaact aacacaaatt
     1261 tctattcgta tgttgcgtta ttatgatgaa cttggtattt taaaacctgc aaaaacagac
     1321 aaatatacag gtcatagatt atactcagtt gaacagattt caatattaca aagaattgta
     1381 ttattgagag attcaaaatt tagcgttgca gaaatagcaa atatcgtaca taattggaat
     1441 gatgaatttg taattaagga attgaataga aaaaagaatg aaatacaaaa ggaaataaag
     1501 caggaacaac aaagaataaa taaaattgat aaatttatag aagctataaa ctgtgataaa
     1561 gatgaaattc attacaatgt tgtctttaaa aagattccaa gttataaaat catatcactt
     1621 agagagataa ttccagacta ccaatctgaa ggtatactct gggaaaagct atctaaattt
     1681 atcaaagaag aacatataga agtatcacgt caatccaaca ataacatagc tttttaccat
     1741 gatgaagaag tcaaagataa tggtgtagat attgaagttg gaatggttgt aaagaagatt
     1801 ggaaagaaca aatctggatt tatatatcgt gagacagaag aaattgatat gatggcatgt
     1861 actatggttt atggtccata tgaaaatata gctggtgcat atgaatcgtt ctgttattgg
     1921 ttagataaaa atagtgatta tcaaatatca ggtataaata gacaaatcgg acataaaggt
     1981 gaacataatg aaataaatcc tgaaaattat cttacagaaa ttcaaatccc ccttataaaa
     2041 gtctaataga ttagtactta aatacttaag tactaatcac ccattgacat cccaaaattt
     2101 tcatgatagt atctaaacgt gtattaaaca tgtttaattt ttttatggag aaacttttaa
     2161 ggggaaaaat tatgaaagca gattcaacaa aggaacaatt aattaatgcc acaatccagt
     2221 tactatcttc acatagagat gtttcaagca ttacggctag agaaattgta gctaaagcaa
     2281 atgtcaattt agctatgatt aattattatt ttaaatctaa agaggagtta attaatattt
     2341 ctattaaaaa atttttaaag cgtcatatgg atgaatataa ggctacacat gatactaaaa
     2401 atttagcacc taaacagcaa ttaagggaaa tactaattgc attttttgat tttataggtg
     2461 aacatagtca atttataaag atttctgtac cctatatatt gatacaggaa gaaattgttt
     2521 atccatttga gatactacct ttaattaagg ctcactatga aaatcagaat aacaaaaaaa
     2581 ctgaagctga atacagaact attgcgtatc aaataacttc atttattcag ttgctttttc
     2641 ttcgttctaa tgcttttttc aagtatagtg gtattgatat tcttaatatt gaacagagaa
     2701 atggtttaat agattttcaa ttagatttat tattggtaga tattacaaaa taatactata
     2761 ttaatttttt atgtacattt ttttatttta tggtaacgtt tgaaaatatt ctcattgact
     2821 aaaaatactt gcattaaata agggggagga attttaatgt tttttacatt tataggagta
     2881 atatcagcaa ttttattgtt gtgtacaatt atatgtggtt tatggataaa acttaataat
     2941 cgtaataatg actctaattc tcactttact ttttcaatac tttcaattgc tttttcactg
     3001 ttaattgtgg tggccttttt agtaagaaat taatgtcttt gttatgctga ggtttattat
     3061 tatagatttt tattattgca tgatttttac tatattatag tatttttatt atattacagg
     3121 gtcttattta ttatagtgtt ttgttgttac agcattttat tatattacag agtcctattt
     3181 attataggat ttttattata ttgtaaaatc ttatttatta taggattttg ttatattaca
     3241 gacttttatt atattgaaat ctcttattta ttgcagtgtt tctattatat tgcaaactct
     3301 tatttattat ggtgttttgt tattgcaaca tttctattat attgtaaagt cttatttatt
     3361 acagttcttt attatactac agacctttat tattacagca tttctattac attgtaaagt
     3421 cttatttatt acagttcttt attacactac agacttttat tattacaatc tcttatttat
     3481 tacagttctt tgctatacta cagaccttta ttattacagc atttctatta cattgcaaag
     3541 tcttatttat tacagttctt tattatacta caaaccttta ttattacaac attttattat
     3601 tttaacttct ttactataat atagtaatat tttaatatct caaaatcata tcaatcactt
     3661 ttcagcatag tctaaaatac ttccactttc taaaattatc tatcatacct atactaataa
     3721 tactttttaa cctttttatt atacatttca attgtttcta acaacctatt tatttccaaa
     3781 actccaatat attttctcaa ttacattgtg ttaatagccc ctaaatcctt tcaaattatt
     3841 tacaatacat tccattaaat aatcttactg ctctataatt ctttttttct ttttaaccct
     3901 actatacatt atttacatca tttttttcaa tacaacattc gttattctat aaccttttta
     3961 cttttactat atacttcttt tctaattaca ccttcaatgt tccacatcat attactttag
     4021 cacaacaatt aatacctaaa aataaccctc taaatcctcc aataatataa tcaatttaac
     4081 tataataata acctctcaca ctatccaaac aatatatttt caaacattac aaaaaaacat
     4141 ccaactatta caatatagct catatccctc ctcccctttc aaatgctatc attgacttac
     4201 aagataaatg aaagtgagga tgatatatat gcttaaagta aaccaattat gtaaatcata
     4261 taaaacaggc agtaaaacat atcctgtatt aaaaaatatt tcttttgaaa tcaacaaagg
     4321 agaatttgtc gcaatcatgg gttcatcagg aagtggaaaa actactcttt taaattgtat
     4381 ttcatgtttt atcccaattg ataatgggga aataattctt ggaaataata atataagaaa
     4441 tttaaaagaa aaggaacttt cagaagttag aaatcaacaa ctaggatttg tattccaaga
     4501 cttcatgcta tttgatggtc ttagtgtctt tgaaaatata tgtgttccac aaattatcaa
     4561 aggtagtcaa gtcttacaaa tggaaaaccg tgcaattaat ctatgtgagc tatttggtat
     4621 tgaacatatc agaaaaaaat accctgccga aatatctggt ggagaaaaac agcgtacagc
     4681 agtagcaaga gcactcatga acaacccaga tttaatatta gcagatgaac caactggtaa
     4741 ccttgatagt aagtcttgtc gtacagtcat tgaatcattt ttaaaagcaa agcaagaatt
     4801 agatgctact atactcatgg ttacacacga tagctttgca gcaagcttct gtgaccgagt
     4861 tattgtgctt aaagatggaa taatttataa agaagttgta aaatcaaatt caagacataa
     4921 attcctagat gaattactag aagttttaaa agaaatggga ggcgctcaag atgacaatta
     4981 ataaattata cagtaaactt cgagcctata attggaagaa ctatacaact ttattctttt
     5041 gtataatact ttctgtggta ttagtaactt catatgctct aatttatttt agcccaacaa
     5101 tacaaaacat acttccaact ggtggagata gcagaaaaca ggctacaatg atattctcag
     5161 ttgctatatt aggatgcgct gtctttacta catacgcttc ttccctcttt ttaaaattta
     5221 aaagcaagga aattggtatt ttcatggctc taggaacaga taagaagaaa ttaaaaaaac
     5281 ttctattttc agagatattg ttaatttcaa tagtatgctg ttttttaggt cttatattaa
     5341 gtattcctgt atcttttggt atatggaaaa tatttcaatt agtgattatt gataccagag
     5401 aaatgacata tcattttgga actcaaggat tcatatatgg atttatattt agtgtatttg
     5461 tgacattatg catatttata ctttctgtaa aatttattaa tcgtacaaat ataattgata
     5521 tattaaatga ccagagaata tctgaaccaa tacaggatgt aaaggactgg tatggtatat
     5581 taggtataat ttttattata ctcggtattt ttctaggata tctcgtacct tcactaacta
     5641 taaacaaatt aaattataaa ctaccatcta tatggagctt tacttatcta ctctctctta
     5701 ttggaatata tatgattatg acatacatag ttgtacatgc taaaagagga aaacatccag
     5761 agaaatacta taaaaacatc atatcaacta gcatgatgag atttatggga agacaaacag
     5821 taaaaaacat gtgtgttatt tcatttttga ttgcaggagc attatttgct gtattctatg
     5881 ttcctaatat ggttacaggt atctatgata ctcttaaaaa taaccctata gattatactt
     5941 ttacctacaa tcaaagagat aaacaggtat caaaaaaaga tattgaacaa ttagcagata
     6001 aacacaatat aaaaattcaa aactatatgg aaattccttc tatagaatta attgttgatg
     6061 gtgtagaatc aatatatcat aaagatggaa ctatcgaaaa ggtatatatg aaaaaaagct
     6121 cctatgatat gttctttaat tccaaagatt tttccaagct aattggcaaa ccaataacta
     6181 taaaaaatgg tgaatatctt gctgttgtaa ataaaggaaa tgaaaatcaa gattataaat
     6241 ataccaaatt tatatcaagc ccagtcacag aaaaagaaat cccctttaga tatgctggaa
     6301 aaataatatt taatggttct ttttttcaaa atagaagtat gaacgcctat gtccttaatg
     6361 aggaggatta taaatcattt actaaagatt tatccataaa aaattacaat aattctatac
     6421 tatttaatgt agataaagat acctatgctt ttgccaatga cttaaagaaa gaaattataa
     6481 aaagaacatc taaggaaatg ggcatgttca gtggttatga tgaatacgta aaaaaacgtt
     6541 atgaatcaac tggaagaaaa tattttatgg atgaagaata tccaagtggt gaaggtcatt
     6601 taaaactaga cccaaacaac aatcagttgt atcttaattg gaaatactat cctaacttta
     6661 aaattttaca aagccaagat atgataaaaa acatggcagt cttcttaatg ttattcatat
     6721 atgtggcaat catatgtttt acagcagtag gaatcattgc atatacaagg ggtgttacta
     6781 tagctataaa tcacaagcag gtctttatgg atttaaatcg attaggagca aacaatgatt
     6841 atataaagtt ctgtattaga aatcaattaa agaaagtatt ttcataccct cttattgcag
     6901 gaagtttttt aatttatctg tttaatttta tgattatgtt aaataatgat ggtagaatcg
     6961 tcaatagtga ggtcaatgct cttttgataa acttaggttt tatagggatt tgtggggttt
     7021 atatgtacct tatatatcta ttaacttata agaaattcaa aaaaattatt ggtattttat
     7081 aataaaaaag gttattagat ttttaaagta ataaaatcat tggtgtttta taataaaaaa
     7141 ggttattaga tttttaaagt aataaaatca ttggtgtttt ataataagaa aggttattag
     7201 atttttaaag taataaaatc attacttttt ataataataa aagcatttgt attttataat
     7261 aagaaagatt gttagatttt ttaataagaa aatcattagc acttcagaat aagtttaata
     7321 gaatagtttt aagtgtaagt atactaatat aaaaaatttt attttgctat ctggatataa
     7381 ttgttatttc attttatata tctaggtagc aaaatatatt atatacttaa ctatataaat
     7441 ataaaacttg aaaggagata aaaaattgac ttcgatttgt atttgtgatg atgaaaaaca
     7501 acaaagaaac atgctgaata aaatcattac aagagaaatg gatttgtggg gctaccccta
     7561 ccaaataaaa gaatattcaa atggagaaag tctagtatac aatctagaac acgaaaatta
     7621 ttacatagat attatttttt tagatattga acttggaaaa agtaatggcg ttgacatagc
     7681 aaaaactata cgaaaacatt caaaagatac tataatcatc tttgtcacag gtttttctga
     7741 ctatgtattt catggatatg atgtaggagc tttgaactac atcttaaaac cttataaaac
     7801 tgaaaaaata tgtaaagtat taaaagaagc actaaaaaaa ttagaacaat ggaaagaaaa
     7861 ctttattaca gttcagattg gaactaatct ttgtaaaatt aatacaaaag atatcctcta
     7921 ctttaaaagt gagctaagaa aattaactgt agttaccttt gataaaacat tagaatacta
     7981 cggaaaatta aatgatatgg aaactgttct tccctctaat tttatacgta cgcatcaaag
     8041 gtatattgtc aatctaaacc atgtagatag tataactcaa acttatgcca aagttcagaa
     8101 tgaaaaaatt cctataagcc gtaaaaaata tcaagaggtt gtaggaaaat ttacaaggta
     8161 tatgttagaa tcttaatttt tgaagttcaa tctctagaat tttatttttg aaacttaact
     8221 ttttaaagct tagtttttag agttttgttt tttcaaattt aattttttga agcttaattt
     8281 ctagaatttc aatctttcaa aatttaattt tttgaagctt agtttttaga gtttttttca
     8341 aacttaattt tttaaagctc agtttctaga atttcaatc
//
LOCUS       DAYAOX010000159         8326 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.160, whole genome shotgun sequence.
ACCESSION   DAYAOX010000159 DAYAOX010000000
VERSION     DAYAOX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.160"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(185..1177)
                     /locus_tag="ACGI57_002773"
     CDS             complement(185..1177)
                     /locus_tag="ACGI57_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9717841.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(1186..2244)
                     /locus_tag="ACGI57_002774"
     CDS             complement(1186..2244)
                     /locus_tag="ACGI57_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFG9717842.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            2369..3832
                     /locus_tag="ACGI57_002775"
     CDS             2369..3832
                     /locus_tag="ACGI57_002775"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HFG9717843.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            3934..4731
                     /locus_tag="ACGI57_002776"
     CDS             3934..4731
                     /locus_tag="ACGI57_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9717844.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            4744..6846
                     /locus_tag="ACGI57_002777"
     CDS             4744..6846
                     /locus_tag="ACGI57_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9717845.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            6914..7393
                     /gene="rlmH"
                     /locus_tag="ACGI57_002778"
     CDS             6914..7393
                     /gene="rlmH"
                     /locus_tag="ACGI57_002778"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFG9717846.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            7542..7853
                     /locus_tag="ACGI57_002779"
     CDS             7542..7853
                     /locus_tag="ACGI57_002779"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9717847.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            7901..8218
                     /locus_tag="ACGI57_002780"
     CDS             7901..8218
                     /locus_tag="ACGI57_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9717848.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
BASE COUNT         3117 a          930 c         1342 g         2937 t
ORIGIN      
        1 ttatagtatc aataaatata gactactaag aatgtaaatg atttgttaga ttaaggtaca
       61 ttagatatgt aaatagaaaa ataaaaagta tgttttaatt tatgtgatat aagttttaaa
      121 cagagaagca agtttgctct tacctctcta tttaaaaata aaaaaatgtt ttttaagggc
      181 tattttattt aaatctttcc catctcaaat catctccagt aaattctaaa atcctaaact
      241 tatctaatat tctagagaga gttctttgag tgtaagtatt tcctatttga gatggtgtta
      301 agttggttga aataatgatt tttttaccag caacaagtct tgtatttaca atattgaaga
      361 tttctccact agtaaaagaa ttattaagtt cagttccaag gtcatctatt attaataaat
      421 cacaatcaaa caagttttta taattatctt cacttatttg attatttgtg tctcttctaa
      481 atttataatc ttcaagaata tctaaaattc taaaagaagt ttgatagatt acaacattgc
      541 ctttatctaa caattccttt gcaatacaat tgcacatata ggtctttcct agtccagtag
      601 aaccataaaa cagtagattt tcactattat ccttcttaaa atcatgaaca aaattttcgc
      661 agatacttaa attatttagc atattttctc taggagatat ttcaccatca gagcctttct
      721 taggagaaaa tatattcaag ttaaaattcg aaaaattttc ctgacttaaa atcctatcta
      781 aattagacat tttataagct tcatttacaa tttcttgttt gaggcaagaa catttttctc
      841 cattaggtaa aaatccttta tctttacaag aaatacattg aaactttatt tccaagtaat
      901 caagaggtat gttactttca gctaaaagtc tttctttctt tacttttaaa gattctatat
      961 tctctttagt cttctttaca atttcatctt tagacttagg atttaaaagt actatcttag
     1021 ctaaactgag acctatttta gaaatttcat catctataga ttttatctca gggattctat
     1081 tatatacctc attttttcta tgttctaata agagttcatt atcatctctt ctcttagcat
     1141 atttagcaag tatttttctt attttatcct cattcattta atcacctact taaacttttc
     1201 tttttgactt tttttaataa tctcatcgag ctcatcagag gtatactgag tgaatgtttc
     1261 gttgaaatta tgaaacttag tttttttgta agtattgttg ttttgagtag tattaatctg
     1321 cttcttatta atattctcct taactatacg ttcttcctct ttttgcttta aatcatttaa
     1381 attttttata tttttttcat tccaattttt aattatacca tttatataag atatacttgg
     1441 gtttgaaata ttcttagact tagaacatgc atttattatc aaatctatat ccatattaaa
     1501 tttatcaaac caggtgtcca taagttcttt ttctgatttt gatggttgtc ttgaaaatcc
     1561 caattcattg aaaatcgttt tatataaaat atatcgttca cttcttacca aaaagctttc
     1621 ttcaacatct tttggcgtat ataaatttga atcataccaa tttctaataa tgccttcaat
     1681 gtatttaact ggctttgatg taccagtttt atctttgacg tattcatatg cgcaaagtat
     1741 catatcgggg ctcatattgt atttattcat tatatccata atactcatct tttcacttgg
     1801 gtcgagatat cgcccaacta ttttatttat agaattaaac atttttctaa tacttggatt
     1861 ttcagaagta gatacaactt tatctgtatt agattttata gatgaattat tgcttaatat
     1921 attctcaata tagaaatttt ttaaatctaa aaattctatt gaataattaa aattatcata
     1981 ttcttcattg tcatgtattt ttataatctt cttttcttcc cagaatttcc aagcagataa
     2041 cacatcagac aatggtatat ttaggttttt ggctattgaa ttattgtcga acttaggatt
     2101 agaagtaata tcacatgctt gtctatatcc taaaagataa acttttacgt ataacccgtc
     2161 cgccattggc ataaatatat ctataaagat attgggaata gtgatttcac ctaaatctat
     2221 ttcattgctt tctttaaaaa acattttatc acctcagatt aaattatatc attttttata
     2281 ttatatttgt ctgtaaacaa ataaaaggtc acaagattag catgtaaata ttttctaaaa
     2341 gtattcattt tgaaaaggag gagatatttt ggttataaaa ttcaaccaaa aagtaagaag
     2401 aacattatca gtagcgattc tatttatggt attaataata ggttgcttta tagaaaagga
     2461 tgacagttta gaaacataca aagaaaaaac taattcaaaa ataaatcagt ataatatgga
     2521 tgttatattt gatgatgaga ctaagaggtt aatgtgcaat cagaatgttg actacataaa
     2581 taatacaaaa tcaaacatag ataaaatata tttccatata tatccgaatg ctttttccaa
     2641 gaaagatttt gctccttttg aaaaaagtga aatgacaagg gcttatccta atggatttaa
     2701 cgaaggatat atagatataa agaatatatt aaataataat agtaaaatgg agtataaaat
     2761 taaaggagac aaaaatgaca tattagaaat agaattaggg aaagagttaa aaccaaatga
     2821 aagaatgtca ttagatataa aatataacgt aaaaatacca aattcaattg gaagatttgg
     2881 atatggagaa aatactataa atgttactaa ttggtttcct atagcttgtg taaatgatga
     2941 aaggggatgg aatttaaaga gttatgaaac tattggcgac ccattttata gtgagactag
     3001 cgattttcat gtgaaactat tgattccaaa taaatataaa attgctcata caggaaaact
     3061 tattgatgat aaacatgata ataagaaaat gctatatgaa atagaagctg aaaacgttag
     3121 agattttgca tttatactaa gtagtaaatt tgatgtaaac aaagtagatt caaagggagt
     3181 agctatcaat acatataatt taaataaaaa gctttctaaa aaagcaactg aagtggcaaa
     3241 agattcaata aatatattta gtgaattatt tggaaagtat ccttacaaag aattttctgt
     3301 tgtagcaagt gactttttta taggggggat ggaataccct atgcttgtta tgatagacca
     3361 aagtttatat aatgaaaaaa atgaattttt actagaatat gttattgccc atgagacagc
     3421 acatcaatgg tggtattctg ctgttggaaa tgatgaaata agtgaaccct ggttagatga
     3481 agcacttact gaatattcta caatagttta ttttgaacaa aagtatggta aagatatggc
     3541 aaacaagctt ataaaaacta tggagataca gacaaaaagt tatttgagtg aaaatatatt
     3601 taagccagca aatgagtata aaaattctac tgaatacagc ttgaatgttt atacaaaagg
     3661 cgcaatagca tttaatgaaa taagaaaaga agttggagat aaagtattct ttgagacttt
     3721 aaatgagtac tataataaat ataagcataa aaatgctaat ggaagagctt ttgtggaact
     3781 atggaatagt aaaggtgtag atataaataa gattataagt gaatgtaagt aaatagtgaa
     3841 tgtaagtaaa ttttgtgcag agtattaaat tttatttaat actctgtttt tttggtataa
     3901 tataatttat aaggcagaaa aagaggtgaa ggaatgctaa agtattgttc agtagggagt
     3961 ggtagtagtg gtaattgcca ctatataggg tataaaaata ctaatatact agtagatgca
     4021 ggattaagtg gcaagagaat aactacaggt cttaaagaca ttggtgtaga tgcagataaa
     4081 ttaaaaggaa tatttataac acatgagcat gttgaccata taaaaggagc aggtatatta
     4141 tcaagaaagt ttgatatacc agtatttgct aatataaaaa catggtgttc tatgaaggac
     4201 aaattaggtg acatcaaaga taaaaatatg aaagtatttg aaaatgataa aacctactca
     4261 ttaggtgata tcatagtaag acctttttca atagaacatg atgcgtctga cccagttgga
     4321 tataattttt atacagagag tgatgaaaaa atgtctatag ctactgatat aggatgtata
     4381 actcaaaata taaaaaaaca tctatataaa tctaaattag tagtattgga atcaaattat
     4441 gaccctaata tgcttatgat gggttcatat acatatgctt taaagaaaag agtaatgtca
     4501 agtactggtc atttatccaa tgaagatgca gctaatttct gcgttgaact tattaatgaa
     4561 ggtacagaat ctatattatt agcacattta agcaaagaaa ataattttcc tgagttagcg
     4621 tatgagactt caaaaggagt attagcatca aatgatatag tagttggaca agatgtaaaa
     4681 ctagatgtac tatcacgaaa tgatgtttct aatgtttatg agatgaagta gaggtgaata
     4741 actttgctaa agagaaattt tgaaaatgtt gaggataata aaattttata ctctataaag
     4801 cagggtatga ttttagcgat acctgctatt atgactggtt caactgctct tgtaattttg
     4861 aatcttccaa taaaggctta tcaagaatat ttatcaggct tgtttaatgg agaggttaca
     4921 aatattctta actttataaa tgattctact ttaggaatta tttcattaat aatattatta
     4981 acaataagct atagttatgg aaaaatatat gggagcaagt atacagtact tgttcctata
     5041 gttgcaatgt gctctttttt agtattttca catggtaatg agcttaattc ttatatagaa
     5101 atatttaaaa caaagtggtt atttacatct ataattgttt ctatgacatc ttctgtattg
     5161 tttgtaaaac ttacagaatc ttttgttaca agtgtaaagt ttcatacaga aggggctgat
     5221 gcagacttta atatggtagt ttcggctata gttcctttca taatagtagt atttttgttt
     5281 tctattttta gagtggtaat gataagtcta attggcagtt caaactttca agatatattt
     5341 tataatttat tttcaaatgt ttttaataaa atgggaacaa accttatgag tgctctgttg
     5401 tttatttttt taatgcactt tatgtggttt tttgggattc atggtagtaa tgtattagat
     5461 accgtcgcaa aaaatttatt tgaaaatagt atggcaatta acataaattt agttaataat
     5521 aatcaattac caacagaaat ttttactaag accttttttg atacaatggt tttattggga
     5581 ggatgtggct ctttactatg tttagtaatt gcaatttttc ttagtgaaaa acgtataaat
     5641 gtaagaaagt tagctaaaat tgcttcaata ccagctcttt ttaatatcaa tgagatgcta
     5701 gttttcggga taccaattgt ttttaactat attatgtttg taccttttgt aattactcct
     5761 ataatcttaa ccgttacaag ttatgtggct atgtctacag gtattgtacc atgtacagtt
     5821 tcagctgtag aatggacctc acctatattt ttaagtggat atatgtctac aggttcaata
     5881 agaggtagtt tgttgcaagt atttaattta tgtgttggtg ttatgattta tattccgttt
     5941 ataaaaatgt cgcaaaatag atatacatat atgtttaaaa ataatataga aaatttaaca
     6001 aacttattaa aaaggtatga attaacaggt gaacagccta atcttctaaa ccataatggt
     6061 aaaataggtt caatttcaaa aatgttggca tcagacttga agtttgctat gaaaagagga
     6121 gaaatagagc ttttttatca accacaagtc aattataatg gaaatgttgt gggagcagag
     6181 ggattactta gatggaaaca ttcaatagga gggttcattt atccaccact agttataatt
     6241 ttggctaaag aagaagggtt cttagatgag cttggagagt atattattaa taaagcatgt
     6301 attgatttaa agaagagtga aaagctattg agaaatcctg ttaagttctc tgtaaatata
     6361 tctccagacc aacttgatga ccctaagtta ccagagcaaa taaaaaatat cattagtagt
     6421 aatgatataa atcctaatat gttaggtata gaaataacag agcaaattgc attatcaggt
     6481 tcacaaataa ttattgaacg tatcaatgcg atacataact tgggaataaa gttaattatg
     6541 gatgattttg ggatggggca tagctctctt atatatcttc aaaataataa ctttgatata
     6601 gttaaattgg atggttcatt agttaaagag atacttacaa ataaaagaag tagtgaaatt
     6661 ataatgacta ttgtaaatct ttcaaaaaat ttagattttg atatagttgt tgagtatgtt
     6721 gaaaatatag accaaaggga taagttatat ggtttaggat gtgaaatata tcaaggatat
     6781 tattatagca aagcaatacc ttttgaagag tttgtagaat atgcaaatca agaaagagag
     6841 atataatttt ataagagctt gtaaattcta gtaatgcata gatttgtaat aaaattaagt
     6901 ttaggagaaa tatatgaata taagtatagt agtagtaggt aaaattaaag aaaaatattt
     6961 aaaattaggt attgatgagt ttaagaaaag gctttctaag tattgtaagt tagaaattat
     7021 agagttggat gatgaaaaag ctccagagaa tttgagtgtt aaggaaatgg aaattataaa
     7081 agacaaagaa ggaaagaaaa ttttagggaa gataaagcat aatagttatg ttatagcctt
     7141 ggctattgat ggtaagggtt tatcctctga agagcttgct aagactatga gtgatttagc
     7201 agttagaggt aatagtagtc tctgttttat aataggaggt tctttagggc tttctgatga
     7261 ggttttagga agggctgatt ataggttatc cttttctaag atgacttttc ctcatcagat
     7321 gatgagattg atattgttag aacagattta tagggcttat aggataaata atggagaacc
     7381 gtatcataaa taaatgatac ggtttttgtt aatttgacac ttaataatat taaaataaca
     7441 aaaaagtatt gaaagtatat tgcatttaag gtagaattaa ataaaatatt ataacattat
     7501 aatgtttata tggttgttaa taaaaatttg gaggaaaata tatgataaaa ataatgttgg
     7561 cctgttcagc aggaatgtct acaagtttat tagttactaa aatggaaagt gcagctaaag
     7621 aaaatggtat agagtcgcaa atttgggcaa taccagaatc aactatacaa aatgaaatag
     7681 aaaaatgtga tgtattatta ctaggaccac aagttagata tgtattacct aaggctcaag
     7741 aaattgctaa gccatataat ataccagtag aagttataaa tatgatgcac tatggaacag
     7801 ttaatggaga agctgtttta aatagagcga tagaacttaa caacactaag taatataaat
     7861 tataatgtat ttttaatata taaattggga gaatcaaagg atggatgaaa agactataga
     7921 aatatctttt aatataatag gttatgcagg tgaaggtaaa ggattagctt ttgaagcaat
     7981 aaaagaggct aagaatggaa atatagaaaa agcaaaagaa ttattaaaag agtctaggga
     8041 agttataaat aaagcacata gatatcaaac agaattaata caaaacgaag ctagtggaaa
     8101 caggactgaa ataagtgtca tacttataca tgcacaggac cacttaatga atggcatgaa
     8161 ttttcaacag ttagctgagg aaatcgttga tttacatttg aaattacaag ataaataaaa
     8221 attaaagata ataaattaag agagttctta aaagatacat tatgtgtagc acactaattg
     8281 ttagattatt gattcaatat ttagtgagta atacattagg attatt
//
LOCUS       DAYAOX010000160         8281 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.161, whole genome shotgun sequence.
ACCESSION   DAYAOX010000160 DAYAOX010000000
VERSION     DAYAOX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.161"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(493..1365)
                     /locus_tag="ACGI57_002781"
     CDS             complement(493..1365)
                     /locus_tag="ACGI57_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9717849.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(1759..2337)
                     /locus_tag="ACGI57_002782"
     CDS             complement(1759..2337)
                     /locus_tag="ACGI57_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9717850.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(2395..3111)
                     /locus_tag="ACGI57_002783"
     CDS             complement(2395..3111)
                     /locus_tag="ACGI57_002783"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFG9717851.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            3302..4387
                     /locus_tag="ACGI57_002784"
     CDS             3302..4387
                     /locus_tag="ACGI57_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFG9717852.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(4542..5201)
                     /locus_tag="ACGI57_002785"
     CDS             complement(4542..5201)
                     /locus_tag="ACGI57_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9717853.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(5418..6710)
                     /gene="lysA"
                     /locus_tag="ACGI57_002786"
     CDS             complement(5418..6710)
                     /gene="lysA"
                     /locus_tag="ACGI57_002786"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFG9717854.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(6728..7921)
                     /locus_tag="ACGI57_002787"
     CDS             complement(6728..7921)
                     /locus_tag="ACGI57_002787"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFG9717855.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHLVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     regulatory      complement(8003..8171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT         2558 a         1410 c          850 g         3463 t
ORIGIN      
        1 tattttattt ttataaagta ttatatttct aaaaaagttt ccctttcccc aaaaatggct
       61 acttaaaaaa tcaagatttc atacctaact actaattcta tgatttttaa atttttcaac
      121 ttctttgtca aagttaaaat aaaatactaa aagacacata ttatagtgaa caaaaaagat
      181 agaaatacat attttaatat aattttaata agatattata tacttaaata caaataaaat
      241 taattttata attgttaatt aaggaaatga tattgtttag agaaataaaa aaagcactct
      301 ccatgaatgg aaatgctttc tttaataaat tagttgacgt agtaaagatg tattcagaat
      361 acatctttat gttttgagtc tcgaaatatt tatttgtatt atttaaacga aaatttattt
      421 tttaagtgat aatcgagtat ttactaataa ggctatgatt ctcaatttca tagccttatt
      481 aatatacaaa ctttactcat acacagaata tcttttccaa atttgctcta aatcatcaaa
      541 atgctgttta atatcttctt tttccttcat cccaacagat atagcaaggg catttgaaat
      601 actaagagct ggaactagag aatctacaaa tgaagccata ttactcttaa ctagaagagt
      661 gttatctgca agtgatgcta ctggcgcaaa taagctatct gttaatgata ttacatgggc
      721 tcctttttct ttagcataat tgacaatttg ataagatttc ttagaataac gagggaaact
      781 aatagcaact attacatctt cttcatttat tcttacaatc tgctcaaaag catctcccat
      841 atccattcta attatatgaa cattgtcaag tattatatct aagtaaaaac ctaaatattg
      901 agctactaca aaagaactcc tcattccaag tatatatatt tttctcgctc ttaaaagttt
      961 gttagaagct tctttaaacg ctctctcgtc aatttcttct agtgtagacc ttatattatc
     1021 tatatcactt tttaaaactt tgtttagtat tgcaaaatca tcggaataat catgtgccat
     1081 ttcaactctt tgtactgtag ttagtttatt ttttatgagt tcttgaagtg cagcttgtaa
     1141 tttaggatat ccactataac ctaatgcatt tgcaaatctt acaactgtag actcacttac
     1201 tcctacggtt tcacccaatt tacatgcagt cataaatgca actttatcat aattatttaa
     1261 aatgtattgt gctataagtt tttgaccctt actcaatctc gtatattgag attgtatatt
     1321 tgatatcaaa tgtttactgt ccttcatttc atttgtttct tccatttttt tctcctttta
     1381 gttatataaa ctattttgtt ttattaacac tgtctttttc tctaaaaatt ttatttttta
     1441 tctcaatttc atcttagttt ttatcatagc ttcttaaact cataaaattc taacttttct
     1501 gtaccttata cttcacatag tattttcgat taaatttatt ataccatctt tccttgcatt
     1561 ttttaatatt tatccaatat ttcttttaaa tttttcaatt tttactcatt taaaagttat
     1621 ctatcttatt ttgcatattt agatgtatat tttgttttga gaataaactg ttgtatactt
     1681 attatttaaa gtatttttaa taggtcacac tatatatatt aactcttaat acaatcttgt
     1741 caagtttttt agtatttatt atgctttttt cttttcaatt aaaatcaatt gtggtgggtt
     1801 attgacttga tttaaaaact tagattctaa aacattaaat tcatcttgat ttaaattatt
     1861 gacaaggtta tatatgcaat ctttttcttc ttgacctcca tcatgccctg tatatatggc
     1921 aatgcttatt actccatttt cttttaacat ttctaaactt aactttatag cctttaaagt
     1981 tgtatctggt ttggttataa ttgaatgttt cgctcttgga agataaccca aattaaataa
     2041 aacacatgaa acttcttctt ttatatattg attcattttt tcatgcccat caaatatcaa
     2101 ttttactcta tctgtatatc cttctttctc tagtttcttt cttgtagact ttattgcttc
     2161 ttcttgtata tcaaaagcat atacaaagcc cttttctcca accttctcag ctagatacct
     2221 tgtatcatac ccatttccca tagtagcatc aattactatg tcaccttcat ttataatatt
     2281 ttctaaataa aatttattta tatctgtaat tttagttaaa tactttgcat atgccataat
     2341 actatttctc cctactatta actttatttc attttttctc aatttatttt tatcttattt
     2401 tattgatttt aaatatttta tttcatcttc tgttaggtat ctatattctc caactttggt
     2461 gtcttttaat actattttcc ccacagaaat ccttcttaaa tttaaaactg gatgatttat
     2521 agctttgcac atttttctta cttgtctatt tctaccctca tgtatagtta tttcacacac
     2581 tgaataattt ttttctttat tttctttagt tacccttatt ttggcaggtg ccgttgtgta
     2641 atcttctatt tttagtcctg tttcgaattg tcttatctca tcatcactta taattccctt
     2701 cacacttgcc acatacattt tatcaatttc atgctttgga tgggtaagct tatatgttaa
     2761 atcgccatca ttagtaagta ttaataaacc actagtttca taatctaatc tccctattgg
     2821 atatactctt tcccttacat ctttaagtat gtctattaca ctgggtctat tgaattggtc
     2881 ttttactgta gttatatatc cctctggttt atttaatact atatatatat aattttcact
     2941 tggttttact tttttattat caaattctac tatgtctttt tcttcatcaa tattaaaaga
     3001 taactctttt actacacttc catttacctt tattcttcct ccaagaatta tttcttctgc
     3061 ttttcttctt gatgctattc cacaagaagc tatatactta ttaattctca tacttaatcc
     3121 ttccttatct taaattatgt gtgtttaata tttttatcaa tgttaatttt gctacattat
     3181 tatactatac tagaccttaa ttaaaaacaa gcaaaaaaat tatttaattt tcctaaataa
     3241 ttgtaatttc ttttcacata ctattaatat gtaatagctg actaaacact attttaagga
     3301 agtgattatt atttcaaagg aaaatataaa atattattta atagtcgtgt ttattggaat
     3361 tttatttttt aaatttatta atactccatc agactttata tctagtattg aaggattctt
     3421 aaagtttttt agcccatttt tattagctat tttacttgct ttacttttaa atccacttgt
     3481 gatgcttttt gagataaaat tcaaagcaca tagactttta gccatttttt tatcttatat
     3541 atttataggt tttattttag cctttgccat taggttatta attccatcaa tagctaatac
     3601 attaaataga ttgataaatg aaatgcctat gtatactgat tatatagata gttttataga
     3661 aaaaaatatg tcaaacatat attttctaaa aactttaatt cctcatatac aacatagttt
     3721 agataattta ttaaaagaag caagtaattt tgttggtaaa attcctaaaa acttcttaat
     3781 ctatacactt agtatatctt ctatgctatt taatatgaca atgggattta tactatctat
     3841 atacattata tatgacaaag aaaaaattgc attgggattt aagagatttc tatattcttc
     3901 tactgctaga aacaaggcag ataacattat agaatttttt aggactactc atgatatttt
     3961 ctatgattat ttacttggta gaatattaga ctctcttatt attggtataa ttgcattttt
     4021 aggatttcaa tttgttatca gaatagaaaa tgctttattt ttagcttcaa taatattctt
     4081 aggtaatata attccctatt ttggtccttt tattggtgca atccctccaa tagccatgac
     4141 tatattatat agtccacaaa aaactatatg ggtcattgca tttctattta tattgcaaca
     4201 attagatgga aattttatag aaccaaaagt tatgggtaat caagttggta taggagctat
     4261 atgggtaata tctgctattt tggtgggaca atctctattt ggtttcatag gagtttttct
     4321 ttcagtacca atagctgctg ttgtaaaaac ttatgttgat aaatatatag ataatcgttt
     4381 acaatagatt atctctactg tgactttaat ttttagttta aaatttaact gctatttatt
     4441 aaattatttc tcaaaaaata tctcttagta agaaaaaatt gctaagagat attttttata
     4501 atatataatt tgttcattaa atttatgcat atattttata cttattgatt atttgagttt
     4561 tgagaatttc caccactatc tgaaccacca gtagtatctt gtggagttgg tgctggtgtt
     4621 ggattactat tatttggctt ttcttgattt tgagaagaac tgttattatt gtcagactta
     4681 ttactatctg attttttatc attgttttta gaactagatt tattattttt actacttcca
     4741 ctataagaag atttccttgc atctgattca actctatacc tatcttctcc agccgtatct
     4801 tttgcctttt tctttctttg ttcctctgct tcgttttgtt ctttttctaa tttagttaac
     4861 tcttctttct ttttagttgc ctcttcttta ataacagagg aaccattttt gattttctct
     4921 ataagtttaa gttccttgat tgcaccctca taatttttta attcttcaaa ctccgtcata
     4981 tttgacattc tcatagcttg catatacata gctcttgcat tctcattagt actatcttct
     5041 tccagtgcag aggataatga ttccatagct tttttatagt tatgtttttc taaatatgtt
     5101 tttccttgct ctactaaatt tatttcatct tttttattaa atgaacatcc tacaagaaat
     5161 atagataata tcgatattaa taaaattact cttcctctca ttaaatccct ccttgtcata
     5221 tactttaaat tttatcataa ttataaagtt tttaaaatat tttatattta ttacaagaaa
     5281 tttgttagta aaacaaaaaa taagcagata tgctacgaac gatacaataa accaaaaatt
     5341 aatttagaga tacatatcct agcaaatctg ctcataataa actttaatat ttaattttta
     5401 ttatatactt ctctatttta tatctctaaa gctaataagt cctcatagct ttgtctctta
     5461 caaattatct tatcatttcc atcaaatact gataccactg ctgctcttgg tattttatta
     5521 taatttgaag acattgaata tccatatgca ccagttgatg ttgttataag tatatctcca
     5581 cttttaatat ccattatgtt tatatctcca attaatatat ctccactctc acaacatttt
     5641 ccagctattg ttacatgatt catagcctca tgattaattt tattcacaat actacactca
     5701 taactagctt ggtacaacga aggtcttaca ttatctgtca tacctccatc tacacttaca
     5761 taaaccctaa catcttttat atcttttata gaacctactg tatacaatgt acttcctgca
     5821 tttgcaacta ttgaccttcc tggttctatt accaatgtag gtacttctat tccaagctct
     5881 ttacatgaag tttctgcttt agtgattatg gtttcacaaa actcttttat agttttaggt
     5941 ttatctcctt cgctataata aacgccaact cctccaccta ggtcaatttc tgttagttgt
     6001 atgttgaatt tttcttttat ttcttttacc aagttcatca ttatatccac tgtatctatg
     6061 tatggttcta catcaaatat ttgtgaacct atatgagcgt gaagacctac aagtttaata
     6121 tttttatatt cacttaattt ttctattgct ctaaataaat ctccatttac taaagcaaat
     6181 ccaaatttag aatctatctg tccagttttt atataatcat gtgtatgagc ttctatacca
     6241 ggagttattc taaagtatat ctcttgagtt ttacctttat cttcacataa tgcttcaagc
     6301 aaatctaatt cataaaaatt atccactaca aatttaccta cacctaaatc taatcccatg
     6361 tttatttctt ctttagtctt attgtttcca tgaaagaaaa ctttttccat aggaaaattt
     6421 gatttataag ctgtatataa ctcgcctcct gaaactacat ctaaacacat ttgttcttca
     6481 tttataagat tacacatgta aagaggtaaa aaagctttcc ctgcataagc aaccttattt
     6541 tttccctctc tagccttaaa atattctata tattctctac aattttgtct aactaaagct
     6601 tcatcaaata cataaagtgg agtttgatat tttttactaa gttctaaaca actcactcct
     6661 ccaatatata attcatcatt atttacagtc atagttccat gaagtttcat aatagcctcc
     6721 ccaaatttta tatattaaat acttctgcta ttttttctat agctacttgc ttatcatcag
     6781 aatttatagc acaagttata cgaatctctg atgtagttat aagttttatc attataccat
     6841 tttcattgaa tatcttaaat actttagcag caactccaga agtatttttc ataccaagac
     6901 ctactaaaga aaatttagtg atattattat caatcactaa atgttcttca tctgtatatt
     6961 tagatactat ctttttacat tctcccaact cttcttttgg aactgtaaat gatacactta
     7021 tcttatctaa aataggtgca gtttggctta tcatatctac acttatacct atagttgcta
     7081 tatcttcaaa taagctagat atattttcag ctttaaaatc ttttattgtt atagaactgt
     7141 catcatcact agtagctaat cctgttatta ctttatcctc taatttcatg tcttttcccc
     7201 ctcttatata tgttcctttt tctgtaccac atgtacgtcc gacatagatt tcaacaccat
     7261 atttttgtgc aagctctata cttcttgaat gcattacttg tgctcctaaa ctcgctaact
     7321 caagcatttc ttcatattca atctcatcaa gtttactagc ctttgaatat tttcttgggt
     7381 ctgtaaaata aattccatca acatctgtat atatctcaca ttttccattt aatttaaccg
     7441 caagagctac agcagatgta tctgaaccac ctcttccaag agtggttaca tcaccatctt
     7501 cgttgattcc ctgaaatcca gttactatca cgacatttcc ttcatctaaa ctatttttaa
     7561 ttctgtttac gtttatatca tcaatttgag actttccatg aagaccactc gtttttatat
     7621 ttaactggta agcattataa cttatagctt tacatccaag tgcatttaat gctattgata
     7681 ataaagatgc tgaaatcatt tcaccagtag acattaaagc atctaattcc ctcttactag
     7741 gttcattact caattccttt gctaaagtta tatattcatc agtggatttt cccatggcag
     7801 atacaactat taccatttgt ggattttctt ttcttcgttc tattatattt tctgctattg
     7861 attttatttt ttcagtatct gctactgaac ttccaccata tttttgaaca attatactca
     7921 tgctttctcc ccctcaagct aaattttgat aaataaaaac gcccaaaggt atactttgga
     7981 cgtgtaaatt tcatagacca agtaaccttg tagctcacca ctttaaaagt gacagcctta
     8041 tgcatattct gcataagtcc agaaaatttg tccgtcaaca aaatttcttc ggctatatac
     8101 cctttcatca attatcaacg cttaccagct cctattgaat actattgaat acagctacct
     8161 ctaccctagg ctcattattt ttaattaatt tattaatata gttttatcat attctatata
     8221 atattgcaac aattatttta caaaatgtgg caaatcgata gttttctttt ctaaagttag
     8281 c
//
LOCUS       DAYAOX010000161         7991 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.162, whole genome shotgun sequence.
ACCESSION   DAYAOX010000161 DAYAOX010000000
VERSION     DAYAOX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.162"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(478..1296)
                     /locus_tag="ACGI57_002788"
     CDS             complement(478..1296)
                     /locus_tag="ACGI57_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9717856.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            1464..2045
                     /locus_tag="ACGI57_002789"
     CDS             1464..2045
                     /locus_tag="ACGI57_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9717857.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2042..2230
                     /locus_tag="ACGI57_002790"
     CDS             2042..2230
                     /locus_tag="ACGI57_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717858.1"
                     /translation="MKIITIGSSLITVLLFLSTMICGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYI"
     gene            complement(2400..4142)
                     /locus_tag="ACGI57_002791"
     CDS             complement(2400..4142)
                     /locus_tag="ACGI57_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloendopeptidase"
                     /protein_id="HFG9717859.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            4571..5947
                     /locus_tag="ACGI57_002792"
     CDS             4571..5947
                     /locus_tag="ACGI57_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HFG9717860.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            6258..6929
                     /locus_tag="ACGI57_002793"
     CDS             6258..6929
                     /locus_tag="ACGI57_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFG9717861.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            7118..7915
                     /locus_tag="ACGI57_002794"
     CDS             7118..7915
                     /locus_tag="ACGI57_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFG9717862.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         2956 a          983 c         1224 g         2828 t
ORIGIN      
        1 aattctagtc atttaaacat aaaatcataa ttgatttatc tgacaaattg agataatatc
       61 ctaattaaat tgtaggtatt taaacataac attataaata atttatctga caaattaaga
      121 taatatccta atcaaattct agtcatttaa acacaacatc acaattgatt tatctgacaa
      181 attaagataa tatcccaatc aaattctagt catttaaaca taacatcaca agtgatttat
      241 ctgacaaatt aagataatat cctgattaaa ttctagtcat tcaaacataa catcacaagt
      301 gatttatctg acaaattaag ataatatcct aattaaattg tagatattta tacataacac
      361 taaaagtaat ttgtttaaca aattaagata atatcctaat taaattgtag gtatttatac
      421 ataacactaa aagtaatttg tctaacaaat taaaatatta tccaattaat ataaaaatta
      481 cttcttttta actgggaagc atacctctgt tacccaatta tcaggattac tatcgttgcc
      541 aggacttaca tgataaatat taaacatagg accatccaca tcatagttat tatctgatat
      601 ccaattacca atagcctcac aagcgtctgc aacttgattg aaatttccat taacaattgc
      661 agttgcagca gtcacacttg gaacagtctt aaatttaaca tgttcagtat ctttatactt
      721 tccagataca gcaacttgta cttcaacatc tacataatct tctttatatc cagtatcata
      781 aaaaactgct ttagaatact ttgggggttc aatttgaaca ttctggtctt ttgtctctaa
      841 aaatgcttga tgccataaca ttccttcgtc ttgataagtt ggtataaccc ttctaagtgt
      901 catcatatat tttggtgcaa aattctttat agttacatca taatttttca taatatcatt
      961 tcctccaatt cttttaatag tggtttctat ttttaatagt tttttctgtg tatcttctaa
     1021 ttgttctttt acttgagaat ggtggatatt tagatatttt atgagacttt ctttatcgtt
     1081 atattctgtc aaaatttctt ttatagaata caagccaaag cccatatctt ttagtgcatg
     1141 gattctgttt gtagtagaaa gttggtctgc actatagtat ctatatccat tagttttatt
     1201 cgtatgagaa ggagttaaaa gtcctatttc atcataatga cgtaacatac gaatactaat
     1261 tttacttagc tttgaaaaat cgcctatctt aaacatgttt ttgtctcctt tataaaggta
     1321 cttttagctt aatattaata taaaccatat cataatgtta gagtcaacta aaattataat
     1381 taaattatat tgacatatga tgaaaaaaat attatactga acatgttcag aactgaaaat
     1441 gttaattgtt ttggaggtct attatgaaaa gtaatgataa agatgctaga gaacgtataa
     1501 ttgaagttac attgaatctt ttaaatgaag tagatgatat tgaggagatt acagttagaa
     1561 aaattgctga gcgtgcaaat gttggagttg gtttaataaa ttatcatttt aaaacaaagg
     1621 ataatttgtt gagtactgcc ataggagatg tgatgtcaaa tataatagca gagctatatg
     1681 acgatagtgt atatactcta agacctattg aagatttaaa aaatttatta aaaaaattgt
     1741 gtgatactgg attacattat gaaaaagtgc ttccttttgt attgaatcaa tgtataacca
     1801 atggagatat gcaagcagaa cttgatattg tccctatgct tcgtaaaatt tttggaaata
     1861 aaaaagatga aatgagtcta agaattattg ccctgcaaat tattttacca atacagattt
     1921 ctgctttatc tacagaatca tttcagttat acagtggtat aaatataaag aataaatatg
     1981 agcgagataa attcattgat attttaatag aaaatattat tggagaggat gttgatgtaa
     2041 gatgaaaatt ataactattg gttcaagtct tatcactgtt ttactttttc tttcaactat
     2101 gatttgtggg ttctggatta agaataataa ggtaacagat gcaagttcta ttaagttcca
     2161 tatgaatagt gctattttta cagggatatt tttattgatt tccacaatcc ttttaattat
     2221 atatatataa aaataataat attattaata aataaagtat cagaaattat aaagacaaga
     2281 gtcttaaaaa tttctgatac tttaccatat aattttatta aacattaatt gtgtgtgaaa
     2341 aaatatcact tatttcaatc taatactaaa tcttaagagt gagtataaag tattagaaat
     2401 taagaagcat tttttagttc aagcggatta tctataccaa aataatcata taaagtagag
     2461 cttatttcct ctaaatttcc atcttggaaa ggtaattgta aatttacctt ttctaccaca
     2521 tctttaaatc cagcatcagt attttgattt aaaaaatcca tatatacatc aacagctttt
     2581 ctgtaatctt cttgtgataa ttgccatatc tgaagtgata catcagatgc aagaccataa
     2641 ctcaagtaat aaaaaggaac ttggaagtta tgagatacaa gtatccaaaa aggagcattt
     2701 ttttctctaa gtatattatt ggaaactcca tattcttcct ctaaatcaaa aaacaattta
     2761 ttgatatcat ttagactcat atatggattc ttgtatataa tttcttgaaa ttcatcataa
     2821 agacaagcat caataatagt atttaaaaca atcgataagt gttctttttt tatagcttca
     2881 gattgtttac caaaaaaatc atcaaaatat ttataaaata gaatttctaa agatgttgaa
     2941 tgaacttcac atacatctat acttggttgc atcttattgt ttaagttaat actgttgtag
     3001 taattataaa aatgaccaaa ctcatgagca aaactagtaa cacctaaaaa agaattatcc
     3061 catgtattaa acaagaaagg tgctttatat ttatttatat aagtagtata tcctccaggt
     3121 gatttgtttt ttccagaaga ataatcgtac aagtcatatt ttttcatata gtcgaagctc
     3181 tcttttagtt ttggagatat atcttcaagt actttatcaa acttcctaaa tgagcgattt
     3241 ttatatactt taatataaat agaagaatca gatggtttag atgaaatctc tctatataat
     3301 ggaactatgt aatcttttac atcttgtcta aattttttag cttcttcttg agaatagtct
     3361 ttgtttaggt tcatataggc ataatctata taattcttaa atccttgctt acttgctatt
     3421 tcagttcttg tctgtataag ttctaagaat atgttaccaa atataggatt gtattttttt
     3481 aagtaatctg aataaatttt cacatattct tcagggctaa gatttggtct tgacaaggct
     3541 tcttcaaagt ctacctctat accatctatt gatactgtag atttagctaa taagtcttca
     3601 tattttacaa ctaggtcttg ctctttttta agcaaacctt caacttcttt agaattaagt
     3661 tttccaatgt tctttagata attaaatgta tcctttccaa gttctttttc taattcttta
     3721 gagaatttag aagttacaaa tctatcttga aagtctttgt atgacatgtc aacatctact
     3781 gatttactca taagatactt atattcctta gagtaaaatt cgtctgatga atctatgtta
     3841 tttctaattt ttgctatagt aagtgtactg taaaaatctt gataaatttt atcaacttca
     3901 ttaaataaat ttaattgttc cttagctgtt ttagctgtta aaagtttgtc gttgcacttg
     3961 ttaatgtttt cgcaaattga ctgtatatta ggacgcttat attccatttt ttcaaaagga
     4021 atagttcctc taacagaatc tttttttgta tcattagtat aatttcttat gtatgatgca
     4081 aatataataa aaactgcaat taaaattaga aaaacaaata ttttcttctt ggatttaacc
     4141 ataaaaatcc tccaatcatg gatatagtta ttaattattt tactatatta aatataaaaa
     4201 atgcatgtta agaaaattta acataaagat atagataaga ttataaataa tataaaaaat
     4261 ttaacttcaa aaaagtatat gaaatttata ctggtaaaat gataaataaa ttttaagtac
     4321 aaaatagtaa aagctatgat agttttagac ctattatatg taaaaaatat agatgttttt
     4381 ggtgcaattt aaattaaaag atatatataa aaatttaaaa tattccaaac agaaattata
     4441 aaaattacta tcattgtctt tatacaatat ttatactata atagaataat aatttagagg
     4501 aaagctctaa aaataaacaa attaagggtc ttaacagtag tgattttatg ttaaggaggt
     4561 ataaagaaag ttgaaaacaa tagttagaag acttattctc tacgcagatg gaatgcgtcc
     4621 tactcaaata atggtatctg ggtttgctgc gattattgta atcggagcat tgttgttaac
     4681 actcccgata gcgtcgcaga gtggagagag tataggctta ttaaatgctc tatttacggc
     4741 tacttcagcg gtttgtgtta ctggtctagt tatggtagat acagcaactt attggagttt
     4801 atttggacag atagttataa taacattgat acaaattggt ggtcttggat ttatgaccgt
     4861 agccacaatg ttttcactta tggcacgaaa aaaaatacaa ctaagagaaa gattattaat
     4921 acaagagtca ttaaatcaag ctgatttatc gggacttgta agacttacaa gatttgtatt
     4981 aataataacc ataacaatag agggaatagg tgcactggtt ttatcaactg tctttattcc
     5041 acaatttggt ttgtctaaag ggatatggta tagtgtattt cacgcaatat cagcgttttg
     5101 taatgccgga tttgacttga tgggcagtgt aagtggccca tttacatcgt taaattctta
     5161 tgtaaacaac tttacagtat caatgactgt ttgcgcactt atagtgcttg gagggctagg
     5221 attccctgtt gtacttgata ttgtaagaaa aagaagattt tcaaaattaa atgtgcattc
     5281 taaggttgta ttgttttcaa ctgctacact tatttttgtg ggtgcattat ttatttttct
     5341 tatagaattt aatcaaaaag ctactatggc ggacttgcct ttaaagggaa aagtactatc
     5401 agcaatattt caatcagtaa cagcaagaac tgctggtttt aatacgcttg atttagctac
     5461 tttacgagaa agtagtgtat ttgtaatgat aattttgatg tttattggag catcacctgc
     5521 atcaactggt gggggtataa aaacaacaac actagctgtt ttaatcatta cagttagaag
     5581 ttttctatca ggaaaatcag atatagaagc ttttgaaaga agattagctc catcaactat
     5641 aaaaaaatct cttggtatat ttgtaatcag tatatcagca gttatttttg gaacattgat
     5701 tatttcaata actcagccta attttacatt agttcaatca gcttttgagg ttacatcagc
     5761 acttgctaca gttggttcaa gtttagctgg aactccaaac ttaaatgctt taggtaagat
     5821 tataataata atctttatgt ttatgggtag agttggttct cttactttat ttatggctat
     5881 actttctggt ggtagaagaa agagtcaacc aatcagatat gcagaaggta aaattatggt
     5941 tggttaagtt accaaggagt agtataaatt tataatgggt ttagagtagc actgtaaatc
     6001 tgttgataaa ataaatgagc ctagatgtaa ttatatgaag aaataaaatc aactaatgaa
     6061 caaaattaat taatgaaata aaaaaacagg gaaataaaat acaaataaaa aggtaaatca
     6121 aaattaaatt aatctgtgtg tataaaatta tatattgtgt ataagttttg tatgtgtaaa
     6181 gtatataagt tttgtatgtg taaacaatta atttaagaga tattatataa attgtgtatt
     6241 agggaggaag tttcgagatg aagcaatata tagtcattgg gtgtgggaga tttggaagtt
     6301 cagttgcgtc tactatgcat cttttaggac atcaagtaat ggcaatagac aaaaatgaag
     6361 attcagttca aagtatatct gacaaggtaa cccattcact tatagtggat gttactgatg
     6421 agcaagcgtt aaggtcatta ggtttaggta actttgatgt agcagtagtt gcaataggtt
     6481 ctgatataag ggcatctata atggcgactc ttatagccaa agaaatgggt gtagagttga
     6541 taatatgtaa ggcaaaggat gaattacaag ctaaagttct ttataaaatt ggtgcagata
     6601 gagttgtatt tccagaaaga gatatgggag taagagttgc acacaattta gtttcggata
     6661 atatattaga ccatattgaa cttgacccag agtattcaat tgttgaaatc gtaactccaa
     6721 atagttgggt tggcaagaca cttatagagc ttgaattaag agctagatat gagataactg
     6781 tacttgctat aaaaacaggt aaaaatataa atgttacacc ttctccagat gaggaactta
     6841 cagccggaag tatcctagtt ataatcggtc aaaatactag tataacagcg ataacatctg
     6901 gaaataaggg gataattaga agaagataat ttactattta atatatattt aattgcaatg
     6961 aaagcagata gtatcatata attatgaata gttatatgat actatttttt taatcgaagg
     7021 tagtatttat ttgtaagatt agataaagag aagttaaatt aaaagtaagg aggctgtgct
     7081 aatataaaaa tttataagtt attagcattt agataaaatg attacaaata taaacagtaa
     7141 ggataatgaa aagttaaagt atacaagagc actattaaaa tcaaagaata ggaataaaga
     7201 gtcaaagttc ataatagaag gatacagaat agtaatgctt gcacttgaat gtatggcaaa
     7261 ccttgattat gtatttatca atgaagaatt tgaaaataag aaagaacatg taaaactatt
     7321 agaagatttg gataaaaaaa acacaaagat atacaagact actaataaaa actttaaaga
     7381 attagtggat acagaaaata ctcaaggaat aataggtgta gtttcattta agaaaaaaaa
     7441 attaagtgaa agtataaata aaaaagataa atttgtattg attttagaca gaatacaaga
     7501 cccaggaaat atggggacta taataaggac tgctgattct gctggagtag atgccataat
     7561 agcactaaag ggatgtgtcg atatatacaa cccaaaagta attaggtcta ctatgggttc
     7621 tatttttgat atgaatataa ttgatgcttc acaagatgaa actgtggaca tgcttaaatc
     7681 attggatttt aatatagttt caagttactt aaatacagaa aatttttatg acaaaataga
     7741 ttatggctca aaagtagcat tggtgatagg aaacgaagca aatggaataa atgaagaact
     7801 tgtatcaaag tctgatattt tggttaagat acctatatat ggtaaagccg agtcgttaaa
     7861 tgctgcgata agctctgcta tactgatgta tgaaataaaa aaatacttaa tttaatgtat
     7921 tgtaataaat ataatgtcat gttataatct tgaattaaat agtagagtat caaaaatagt
     7981 caatatatat t
//
LOCUS       DAYAOX010000162         7990 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.163, whole genome shotgun sequence.
ACCESSION   DAYAOX010000162 DAYAOX010000000
VERSION     DAYAOX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.163"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            43..1836
                     /locus_tag="ACGI57_002795"
     CDS             43..1836
                     /locus_tag="ACGI57_002795"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFG9717863.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIAPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(1917..3299)
                     /locus_tag="ACGI57_002796"
     CDS             complement(1917..3299)
                     /locus_tag="ACGI57_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFG9717864.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            3557..3904
                     /locus_tag="ACGI57_002797"
     CDS             3557..3904
                     /locus_tag="ACGI57_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFG9717865.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            3922..4956
                     /locus_tag="ACGI57_002798"
     CDS             3922..4956
                     /locus_tag="ACGI57_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HFG9717866.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(5005..6054)
                     /locus_tag="ACGI57_002799"
     CDS             complement(5005..6054)
                     /locus_tag="ACGI57_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFG9717867.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            6329..7864
                     /locus_tag="ACGI57_002800"
     CDS             6329..7864
                     /locus_tag="ACGI57_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717868.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
BASE COUNT         3118 a          964 c         1366 g         2542 t
ORIGIN      
        1 tatatttagt tattcaaact attatttcga ggaggtcttg ttgtgaatat aaaagataga
       61 ttaagtggat taagaaaatt tatggaagaa aaaaatatag atgcctatat gattccatct
      121 tctgataatc accaaagtga atatgtagga gattacttca aatcaagaga atttatatca
      181 gggtttaatg gttcagcagg gacggttatt gtgactaaag acgaagctgg gttatggaca
      241 gatggaagat attttataca ggcagaatct caattagaag gaagtacaat aaaattattc
      301 aaaatgggac aagaaggttg cccaactaca gatgaatatt tatataaaaa tattcctgaa
      361 ggaggaactt taggatttga tggtagagtt atatctgcta gagaaggagc tacattagct
      421 gaaaaattat ctaaaaaagg tataaagata gaatatcagt atgatttaat agatagtata
      481 tggccagata gacctgcttt atcagatagt aaagcatttt tattagatgt taaatattgt
      541 ggagaaagtt ttagctcaaa attagctaga ttaagagaaa aaatgagtga aaaaggtact
      601 tctactcatg taataactac tcttgatgat atagcatggt tattcaatat aagaggtgga
      661 gatgttaaat ataatccagt tgtactttct tatgcagtaa taactttaaa agaagtttat
      721 ctatttgttg atgaaagtaa gttaaatgaa gaaatactaa atgaactagc taaggaaaat
      781 gttcaaataa agccatataa tgatgtgtat gaatttgtaa aaaatataga caaaactgaa
      841 aaagttttat tagatggaac taagcttagt tatacaattt acaataatat accttgtgaa
      901 gttgagaaag ttgatgagtt caatccagta atgtttttta aagctcaaaa aaatgaggta
      961 gaattagaaa atataagaaa ttcacatgta aaagatggtg tagctttcac taaatttatg
     1021 tactggttaa agaaaaatgt aggtaagatg gaaatcactg aaataagtgc tactcaaaaa
     1081 ttagaggact taagaagaga gcaagaaggt ttctttgagc caagtttcaa tacaattgct
     1141 gcatataaag agcatgctgc aatgatgcac tatagtgcaa ctccagagtc aaattataaa
     1201 ttagaggcag aaggattatt tttagtagat tcaggtggac aatattatga tggaactact
     1261 gatataacta ggactacagt tttaggacct ataagtgatg aattaaaact acactttact
     1321 tctgtagcaa gaggaatgat aaatctttct aaggctaagt tcttgcatgg ttgtagagga
     1381 tataacttag acatactatc tagaagttgc atgtggaata tgggaattga ttatcaatgt
     1441 ggaactggac atggtatagg atttgtgcta aatgttcacg aagctccaaa tggatttaga
     1501 tggagagtag tacctgaaag atttgatagt gcagttcttg aagaaggaat ggtaactact
     1561 aatgaaccag gtatatatat agaaggttct catggtataa gaacagagaa tgaaattgta
     1621 gttagaaaag ctgaaaagaa tttctatggt caatttatgg aatttgaagt tgttacgtta
     1681 gctcctattg acttagatgg tatagcacca gaattaatga ataaggatga aaaagattat
     1741 ttaaattggt atcataagtt agtttatgat aaaatatcac ctttcttaac tgatgaagaa
     1801 agagaatggt taaaagttta cactagagct atatagtttg tactagatac aataaaaatc
     1861 gaggtattat aatgcctcga ttttttattg tatattttat taactttaaa aaatttttaa
     1921 tttgataatt ctactaattt tttattcatt ataagtagta taattccaaa tatcaataaa
     1981 aatgatggta tcactatgaa gacttgtaac ataccaagtt ggtctataat agcttgcgta
     2041 tagccagata atttatttgc tgcaaatttg cctaaaaacc aaacacccat taggaatgaa
     2101 atatactttt caggcgaaag tttatttacc ataccatacc cgactggaga aaagcaaatt
     2161 tctcctatac tttggaatgt taaaaatcca actacccaaa atacacttgc tttgtgaatt
     2221 gaattagggt ctgttcctgt ttgtataact gctactatca taaacaaaaa tgctatggct
     2281 aatagtataa aacctaagct catttttttt ggcacactta aatcgccttt tttagtttga
     2341 gataatttta cccatactaa agcagagata ggtccaagaa taacacaaag gagaccattg
     2401 tatgaatcta tccatgaagt aggaatttga aaattgccaa cagtaagatt tatgtgttcc
     2461 tttgtataga gtgcaataga catagaggct tggttatatg ctatccaaaa aaatactgtg
     2521 aaaaaagcta acaccaatat tactattatt ctttttttct caatcaaagt taaaggtctt
     2581 gaagcgagca ttttcttata ttctagtgat tttcttatag actctctccc gttaataggg
     2641 tcattattca aaggtttata aaactttctg tttccaaata taaagaagaa taaacctatt
     2701 agaaccgtaa ttgatgaaaa taaaaataca tatctatatc catacatact tatagataat
     2761 ccgcttttca tagcaaacca ttcattagca actaaaccgc atattaaagg gccaaataaa
     2821 gtacctagat tagtagccat atacgtaata gagaaagctc catcttttct tgggtcatca
     2881 ttggtatata agtttccaac catagtatgt aagtttcctt tataaaaacc tattcctaat
     2941 ccagataaaa atatcatgat gtttatatcc atcttactat ttgctatgta tcctagaaaa
     3001 taaccaattg aaaccattag cattccaagt ataatacagt ttttgggacc taaaaatcta
     3061 tcactgaccc atccaccaat aactggagag aaataagtcc atgctaaaaa agatgcaagc
     3121 attgatgcgg attctgctgg tgttaaccct aaaccacctt tggagacttg ttcagctaaa
     3181 aacaataata ataccaattt cataccatag tatgcaagtc ctacaaaggt aaatattgaa
     3241 cagcagaaat ataaatctct aggttgttta agtttttctt gcgtttttaa aactgacata
     3301 tgtcgagaac ctccagataa atagtttaac aaaattttac catactatat gagtatatgt
     3361 ttttttcata tagacgtcaa cttgtaagat aatttaatta agtcgaaata tatgtaaaaa
     3421 tagtatcatt tttgtgtatt cagtaatatt tttttatgat aatatatgtt attattatat
     3481 taaaatacaa acatatttat ggaaaggata tgccaattta gtagttagat tgattgtttg
     3541 gcaggtggga tgagctatga taagaaagaa atctgaatta caagtagaag taaaagaaaa
     3601 tttaaatggt ggtataggaa aggttaagct tgaaaacata atacaaaatg aagagcttaa
     3661 aggaaaagga agactattta aaagagtagc tttaccagta ggaagttcaa ttggtgtgca
     3721 tgaccataca acagattttg aagtatatta tatactaaag ggaaaaggta aagtatttga
     3781 taatggggaa tttgtagaag taaatgaggg tgatgtagtg tatacagctg atggcgaaaa
     3841 acattctata gaaaatatag gagaagaaga tcttgagttt gtagcactag ttttatatat
     3901 gtaaaatatg gaggaggagt catgacaggc aagatagaag aaattaagga caagataaat
     3961 agattttgca actccaattt aaatcaagaa tacaaagata taagctttaa aattttgcaa
     4021 aaccttttag ataataataa agagataatt cattcttcta gagctgatat atggtcttca
     4081 gctatactaa atatagtttt agaacaaaat cttttataca ataagaagca tccactacat
     4141 ataacaaaaa aagagttttc aaaacaaata ggtgtttctt taaatactat aaataataaa
     4201 tcgtctttaa taaaagatgt ttgtagtatt gactttttaa atcaagatac tatagctatt
     4261 tctaatgtag aattttgggt aaaagagact aattccaaat caaagtctgt agatttagat
     4321 ttagaaaaga agaaaatact atataaaaga tatattaagc tatcacaaga tgcgaatagt
     4381 gatattgaat caattagata tatggaggaa gctgtaaata taggtaaaag tatgattact
     4441 aaagaagata aagaacttgg attttggaaa ggtttatcta ccagagccta tatgatagca
     4501 cttgaatccc ttgctagaaa gttagaaagt attaataatc taaaggagtc caaaaaagta
     4561 ttagaatatt taatagaaat taatcctagt gatgagcagg gaataagata caaattatta
     4621 aatgtattaa ttagattaaa tgatagaact tcaataaata aattatttga actttataaa
     4681 gaagaaaaat ctgctacatg gatgtacagt aaagctcttt attactttaa aaataaaagt
     4741 atgtttttgg caaatgattc tattaaggtt gctagaagta aaaataagta cgttggatta
     4801 tatttgttag attggcgaaa tgcttttggt agagaatttg ttacagagga agaaaaggca
     4861 gaagctgtct attactatga tgaaaacata gtgatttgga atgaagttaa aggttctatg
     4921 gattggctat taaaaaaaat gttggaattt tcataaaaaa aactactcct tacttttata
     4981 taagtcaaag gagtagtttt taatttatac atctagtttt aaatcttcag gttttattag
     5041 accttttttt ctcataaatt ctgaaatcaa caaagttaca atagcaggaa gtataaaatt
     5101 aagtaaaagt ataccaaccc acatagatat acctcctttt ccagcacttt gcatagcagt
     5161 tactgttcca aattgtccaa ccaatccaca agtacccata ccagaaccaa taggaatatt
     5221 ctccattttg aatattgttg tagataatgg tccaagtaat attgaagcta cagtaggtgg
     5281 tatccaaatt ttccaatttt ttattatatt aggtacttgc aacatagatg tcccaagacc
     5341 ttgtgccaca aggccaccaa ctccattttc tctaaagctc ataacagcaa agcctaccat
     5401 ttggcaacaa caacctacag ttgctgcacc accagcaaga ccacctaaac ttaacatcat
     5461 acaaatagct gcactactta taggtaatgt aagagctatt ccaacaagtg cagagactaa
     5521 agctcccatt attataggtt gcatagttgt agcttgcatt attatatttc caaatgaagt
     5581 cataaaagca gaaacagctg gaccaataag ttcagctaca agtactccaa ttagtattgt
     5641 tacagcaggt gtaaccaata tatctatttt tgtttctttt gaaactaatt ttccaacctc
     5701 tacagcaaaa agtgtagcga caaatgctcc aacaggtcct cctaattcat taccacaagc
     5761 tccaacaagt gcacaagaaa atagtacaag tggaggtgct tgaagtccat acgcaatagc
     5821 cactgctata gctggaccag taacctgttt agcaagaggg cttataactt cagtaaaaag
     5881 agaaatattg aaattatctc ctaatgtatt gaaaattgtt ccaattaaaa gggacgaaaa
     5941 aagaccgagt gccatataac ttagtgcgtc aataagatac ctttgaacag aaatttcaat
     6001 attttttctt ttagcaaatg atttgaaatt tcttttttta taattgattt ccaatttaaa
     6061 atcctcctca tataatataa gtataatttc tttttaatta attatataac ttttcagctg
     6121 acaagacaaa tgttatttca ctaaaattag actattgtaa tagtaagaaa ttaacattaa
     6181 ttttagtttt aaattttaaa tttatggttt ataattagta tagggatatt ctgaaatata
     6241 ataattttcc agtttatccc aatatttaac ttaggtagat tgcttacaaa acaaatgatt
     6301 atatattagg ctaaaaggaa gtggatagat gatatttgta aaaaataaga aatacagaaa
     6361 aattgccata attatatcta ttcctatatt gcttgttgcg cagtactacc tttttaaatt
     6421 tggaatactt agaccaatgg taaagggagt agaaattgaa attgtagatg gggaatatgt
     6481 taaagatata gataaatatg tagtaaaatt aaatgaaaca gttactttgt ctacaggtga
     6541 atatattaag gttccttcgt atgctaaaaa tccaaatatt tggtttaatg tattggataa
     6601 ctcagggact ttaaaaatag atggaaataa aatgacagca ttaaaggttg ggtattcttc
     6661 tgtgggtatc atgaaaaatt caagagtatt aaaaaaggct actataaaag tagtagaccc
     6721 agaggtagaa tcactagata tggagattaa tggggattta aaacatgttg gtgatagtgc
     6781 tactatagaa agtacaatag aagttgatta tgataagttt aaaaaatcat ataagccagt
     6841 ttataagagt agcaatgaaa atattttaaa aatcaaagga aataaagtaa gtgcagttgg
     6901 agttggtaaa gctacaatat atgctaagtg tggaaataaa gaaattgaga gaacatttag
     6961 gattcaagct agagttgctg aaataaatat ggatgatata aaaatagaag ttgaccagaa
     7021 tgaaaaattg aaaccagata taataacttc tccgcctggt ttagaaccac caaaaatata
     7081 ttatgagtta gtagattcaa aattacctgt agaaagagca ataagtttaa gtagtaatgg
     7141 aaatgttgta gggcttagag aaggtaaaga tagggtgaga ataacttgtg gagataaatc
     7201 taaagtaata acagtaacag tagtcaaaga aactataacg aatgactata tacaaaactt
     7261 ggttggttca cataaaatag taaacaataa agttattatt aaacttgaat gggattatat
     7321 gaaagatgtc tttgattatg atatatatct taaagataag ttgtcaggag aatcatttaa
     7381 gaaaattaaa tctataacta taaatgaaag tgatttaggt aagtctaaca aggtatatgc
     7441 aactatagaa gtagatatga agggtaatca aaatatagat tttgatattt atgtcatagg
     7501 gaaaaaggat tcagatacaa ctaagccaag taatgtaata aatattacca atggaaatgg
     7561 aaattcaaat aatgataata atattaaaga tatgagggta gaaaatatat cagctaatgt
     7621 tgatagagat aataatgtca ttagagttaa ctggagtagt atatctcata aggactgtac
     7681 ttacagtata tatgtaaaaa ataatactaa tggagatgga gaatttactc tatatcaaag
     7741 tgatatacag ggaaatgagc atactataag tattcctgaa ggagatttaa atctagatat
     7801 ttatgttgtt gcaaattata atggaaatag ctctcaaaat agtgatgtta taaatgtaag
     7861 atagatttgt atacaaattg aaagtgttga gtttatttaa atattaaatt aagaaaaagg
     7921 atagtatagg gtaattatac tatccttttt tgttacaata tataggttta aaatctctag
     7981 taatataaat
//
LOCUS       DAYAOX010000163         7969 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.164, whole genome shotgun sequence.
ACCESSION   DAYAOX010000163 DAYAOX010000000
VERSION     DAYAOX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.164"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            294..965
                     /locus_tag="ACGI57_002801"
     CDS             294..965
                     /locus_tag="ACGI57_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9717869.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            1068..1616
                     /locus_tag="ACGI57_002802"
     CDS             1068..1616
                     /locus_tag="ACGI57_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717870.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            1878..2186
                     /locus_tag="ACGI57_002803"
     CDS             1878..2186
                     /locus_tag="ACGI57_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717871.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(2348..4138)
                     /locus_tag="ACGI57_002804"
     CDS             complement(2348..4138)
                     /locus_tag="ACGI57_002804"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717872.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(4138..5865)
                     /locus_tag="ACGI57_002805"
     CDS             complement(4138..5865)
                     /locus_tag="ACGI57_002805"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717873.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(6110..6376)
                     /locus_tag="ACGI57_002806"
     CDS             complement(6110..6376)
                     /locus_tag="ACGI57_002806"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFG9717874.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILTIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            6674..7786
                     /locus_tag="ACGI57_002807"
     CDS             6674..7786
                     /locus_tag="ACGI57_002807"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFG9717875.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
BASE COUNT         2811 a         1174 c         1040 g         2944 t
ORIGIN      
        1 taatttttaa agtcatttat tatatttagt catcctatat aagttttaat acatactatt
       61 gttttcaaat ataatatact acttttacag ttttgtattt taactaattt caattagttt
      121 taattaatct gcctttaatg ttaatcatgc ttcacacttc aataattttt tatatttttt
      181 aatcttatct gcaatttaaa ttgcaaaaga ttgaccttat tttcactatg ttgtatgctt
      241 aaaattatat aagtttttgt atttttatat tgtttagggg ggtattatta agtatggcta
      301 catttaaaag attacaagaa caagcatatg actacttaaa agaattgata ctatctggag
      361 aaatgataga aaatgaaata tattctgaaa caaaacttgc tagtgaaata ggtatctcta
      421 gaacacctat aagagatgct cttcaaagac ttagtcaaga tggatttata gatataatcc
      481 caagcaaagg atttagaatc catcaaatta cagcaaatga aattgtagag atattccaaa
      541 tacgctctgc aatagaagga ttttgtactt ttttaattac aagtcaatac aaagaagcta
      601 gagcagtaga aactataagt aaattaaaac atcttttaga taaacaaaaa gatattttat
      661 taggtgataa aaatttaaac tcatttgcag aatacgatac attatttcac acaactatag
      721 tttcatatgc tcaaaatact gaatttgaca aaatgtttaa caattatatg tacagaataa
      781 aaaaattagc tctcgattca ttgtcacatg aaggaaggct tgaaacaaca ttaaaagagc
      841 acacagatat ttttaataat attgcaaatg gatgtattga agatatttac agaactactc
      901 tcattcatat ggaaactcct aaatacatta atttagaaga tttttgtaac aataagttat
      961 tttagtatta tactttcatt tattaaaaat atatctatct caaattaatt tcctaattta
     1021 taaaaaatat tctataatta tattgtaact aatacttatt ctaaggagtg atttttttga
     1081 acgaagatgt tgtaactaat gaaactattg acaaagatta tgctattgaa gaagttcgta
     1141 tggcatgcaa acattttggt gatttatatt tttatttttc caaagtgtta tttgaagagt
     1201 ttggtgaaga taaaactagc gaaatattaa gaaaagtttt atttgaaagg tctgaagaaa
     1261 gagctattgc tatgagagag agagctcatg aaaatggaga tgaattaatc gcagacaata
     1321 ttattagcac aactgatgtt ccatttctag gatgggttcc tgaatttgaa gagttacatt
     1381 gcccctatgg agcttcttgg cttttaagat ttgaggaaaa tccttggttt aaaaaatttg
     1441 cttctcttta ctgtgatgtt acagatacta ctgtagctga agtatttaca ggagatacgt
     1501 cccataaaat aacaaaaaac attctatggg gagataaaag ttgtgaaaga atatatttcc
     1561 atgatgacaa ggtatctgaa ggtaaatata cttatggaaa taaaaactgt aaataaatga
     1621 tttaaatttt ctatattcat atactatatt ttgtactttt tcatataaat attataacta
     1681 gtcttttatt ttacttttgt ttaacctaaa gataatttac ttttaatcta tatgaattat
     1741 catataaata atcagaaagt tatctttata aattatagtt ttagatttat aatttataaa
     1801 atatttatgt tctttgtaaa attttttaga agcttctaat gttatgctat aaaactatct
     1861 tgagaagggc tgattgaatg tttaataaaa tatatgaaaa gaaagaattt atagtttttc
     1921 aagttaaaaa aggttatgta gtttataata ctagaaagag ttttgaagaa ggtcatacac
     1981 atttaaagca ttttgaagca gcaaagacag ctattgattt agcaataaat aaaaagattc
     2041 ctagaagcaa agatggatat tatctgacta gtttaattcg cataagtgat gatggttatt
     2101 acattgataa gttaagcgaa ttgttatatg ttagagaaca aaaaggtaaa aaagaaaagt
     2161 actgtaattc tggttatcag atgtaacaag actagttgtg aattataagt accaatcata
     2221 agtaccttta gtcttaaaat caaagagtat cagtaaatct ataaaaaaac aacttactga
     2281 tactcttttt aattatatga ttaatatttg aatttgatac aaaattttat acaaaagttt
     2341 atgttatcta ttctgattct ttgtctgcaa attggctatt ataaagcttc tcataaaatc
     2401 cacccttttc cattaattca tcatgattcc cctgctctat aatacttcca ttattcataa
     2461 ctaaaatcaa gtcagcattg cgaattgtag aaagtctatg tgctataaca aaactagttc
     2521 taccattcat aagatttctc attgcttttt gaagcataag ttccagccta gtatcaacag
     2581 aactcgtagc ttcatcaagt atcaatattg aagggtctga tataaaagct ctagctatgg
     2641 ttaaaagctg tttttcccct agagatatat tagatgcttc ttcatttaaa aacatattgt
     2701 atccatctgg aagagtggtt ataaagtgat gaacatttgc tacttttgct gcttgtataa
     2761 tttcttcttt agtagcccca aatctaccat attctatatt ttcaaaaatg gttccattaa
     2821 ataaccatgt atcttgaagc accataccaa acattgaacg caaatcttta cgtttcatat
     2881 ctcttatatc tactccatca attttaatag cacctttctt tacatcataa aacctcatca
     2941 ataagtttat taatgttgtt ttacctgctc cagtaggtcc aacaatagct accatctgac
     3001 cactttttac ttctgcattt aaatcttcta ttaaagttga attttctcca tatccaaaat
     3061 taacatgctc aaatgtaaca tttcctttta cattttctat cttaactgga ttttcaacat
     3121 caggtatttc ctcttcttcg tctaaaattt caaatacacg ttctatagct gcaaaagaag
     3181 attgtattac tgtagataac tgagtcattt gtgaaagtgg ctgatttatc tgccatatat
     3241 aacgcacaaa tgcttgtaaa tttcctactg ttattcctcc acttactgca tatatagaac
     3301 cgacaactgc cacagctgca attccaaagt aagttactaa tgaaaccata ggattcatca
     3361 tacttgatat aaattgtgcc ttaaatccat tttcacaaag ttcttgattt acttttttaa
     3421 attcttttat agaatcttct tctttaccat atagttttat ttcattaaat ccagtataca
     3481 tttcttgtac ttttccattt aattttccaa gagcattttg ttgcaatgaa aatctacttt
     3541 gagatttttt tactacaaat ttagatacaa aataactaat tggaattata aaaagagcta
     3601 taagagccat atatatatta attgtaaaca tcattgaaag tgccaaaatt atgactaaga
     3661 ttgagtttac aatttgcata aaactctgct gcaatgcatt tgatattgta tctacatcat
     3721 ttgatatacg acttaataca tctccaaaac tattactatc aaaataacta ataggcaatc
     3781 ttcttatttt attttcgact tcatttctta aatctctcat tgtgttttga atagcattcg
     3841 taagtagaaa acttgaagta tacgtaaaaa ttgcattggc aaaatatata catgctaaaa
     3901 tcaccaatat ttttgttaca taatcaaaat ttacggaagc ccctggtact ccttttgcga
     3961 tactaattac atctttagtc aattgtgtta ttatcaatcc ttcagtttta ggtgcaagtg
     4021 cattcatagt agctgctgaa attatacata taatagctat aataaaggaa aatttatatt
     4081 tctttacaaa tggaagaagt ctaccaattg ttttcttaaa acctttagac tttttcatta
     4141 ttctaattcc tcctttttta actgagatgt tgcaatttca tagtatattt cacagttttt
     4201 taataattct ttatgagttc ccattcctac aacttgtcct tcattgagta ctataatctt
     4261 atcagcatca ataatagaac taattctctg ggcaacaatc aatacaattg catcttttgt
     4321 ttccttcttc aatttagcac gaagtattgc atcagtttta aagtcaagtg cagaaaaact
     4381 atcatcaaaa atgtatattt caggtcttct tataatagcc cttgcaatag aaagtctttg
     4441 tttttgacct cctgatacat ttgctccacc ttcactaatc aattcatcaa actttctagg
     4501 tttttttgat ataaactcat atgcttgtgc aacttttgcg gaatattcta attctgactc
     4561 tcctgcctta tgtttcccaa atctaatatt atttgctatg ctaccagtaa aaagcagtga
     4621 tttttgaggt atgaatccta tcttttttct taaagctttt aaatcatatt ctcttatatc
     4681 tactccattt atcttaatgc taccttctgt tacatcataa aatctaggta tcaagttaat
     4741 tagagtactt ttcccactac cagtacttcc tataaaagcg actgtttcac ctgtttttgc
     4801 agtaaatgaa atatccttaa gtacagaagc ttctccatct ggataggtaa aagttacatt
     4861 atcaaattct atgataccct tattttcagt atccttaatt ccattttcag gattttttat
     4921 caatggttct tcatttaata gttcttttat tctatttgca gatacctctg ctcttggata
     4981 cataataaat accattgaaa aaagcataat tgaaaacatg gcatggaaaa gatattccaa
     5041 aaatgcaacc aactgtccaa cttgaagctc tccaatattt atcttctcac ttgaaatcca
     5101 aaatacagcc aaaacagcta tatttaatag taaaaagaat gctggttgag taattgacat
     5161 aagcttaaat agtttcttag aaacattagc ataatatgta tttgtttttt caaatcgctc
     5221 tgtttcataa tcatcatttc caaaagctct aattactctt attccagtta aattttctct
     5281 cgatataagg tttaatttat ctagtccttt ttgttgtttt gaagatattg cctgagatac
     5341 ttttgctatt attgctactc caataattat aaaaggtact gatatagcca atactataga
     5401 aagttccaca cttgttctaa ttgtcattat tatacttata atgaacataa ctggagttaa
     5461 taaggcagtc cttagtagta tattaacaaa ttgttgaatt tgaaatgcat cattcgttgt
     5521 tcttgtaatc attgaagaaa ttccaaattt attgtattca gtatgtgaaa attcttgtga
     5581 tttctcaaat atgtcatttc ttatatctct ggttacactt gttgatacct tagcagaaca
     5641 ataacccaac aatatactac ctacaacccc tataatagaa ataataacaa tcactattcc
     5701 catttgtttt atgtaagcta tattctgatt tgcaatacca ttgtcaatta ttttagctat
     5761 tatagttgga atccccaact cgactaatgc aaaactaaat atagccagta tgtttaatac
     5821 tattaataac ttataattct ttaaataact taaaattaat ttcatatttt tctcctttct
     5881 taatttttga gatagtgtaa tcatgtaata atactaaatt tataaatgct taattttata
     5941 aaaaaaggag aaaagagtta acgcactctc ttctccagca tattcttttt aatacaccct
     6001 ccgacaagtc gcttatatgt taacatattt gacaagtaat gtcaattcat atagccaaaa
     6061 gtcttttaaa tgcttcgttt cataacattg taacaaattg gcatgtaatt tatttttgtt
     6121 ttttactgta ttccaatttt acaagttctc caatattact agctttctta aaattcaaat
     6181 tagtcttatc tataccagac ttaaatagtg aaatccctct ccccaatatt attgtcaata
     6241 tatatatata ataatcatca attaagttat ctttcataaa taattttact atatcgcctc
     6301 caccaactat ccaaatatct tttttatcct tctcttttcc aatttgctca attaattttt
     6361 taggttctaa attaatttat agtgtgatta ttctatgtaa acatcacttt tttactcatt
     6421 caataatacc ctattaataa atcctcaaac taatttaaat aaactttgta taaaattaaa
     6481 aatatattat ataaataatt taaaactaca ctaacttatt gaaaattcta attattacaa
     6541 ataaatattg ctctctgtta acgctagttc tataatataa ttataagcta ttaatattat
     6601 aaagccgata ttactaatat aataattata atacttatag ttaatttact tttgttgaaa
     6661 ggtggtaaat aacatgaaaa tttcagaagt cgctactcca aattttttgc tagatttaga
     6721 ccagttagaa aagaatatag ataaaattca acaaatttgt acaaataata ataaacaact
     6781 ttggcctatg cttaagacac ataaaagtac ttacatagcg agacttcaaa aaaatgcagg
     6841 tgctagtggt tttctagttg gaacacttga tgaaggtgaa gctctgataa aagcaggttt
     6901 tgaagatatt atgttggctt atccttacat gggagaagta aatataaata gaattgtgac
     6961 tatggcatca aaaataaatt taatctgtgc tacagataat atagaatgtg ctaaggctta
     7021 tagtgaagct ttttctaaat caaaaataga gtgcaagcta cttattattg tagattgtgg
     7081 cttacaccgt tttggggttg aaccagaaaa agtggttgaa cttgctactg aaatttctaa
     7141 gctagaaaac atcaagattg ttggtataag ttcgcatcca ggtcaagtgt atggttgtag
     7201 ttctccagaa ggtgttccag atgtttgtgt tcaagaagat tcttctatgg agaaagcata
     7261 caaagctcta actgataatg gatttaatat agaaattgtc gcttctggta gtactccaac
     7321 agttgaatct gaagctgctt ctaaaactat aactgctgta agacctggaa actatgtata
     7381 ctatgatgct atacagatag gtttaggttg tgctactgaa gatatgtgtg ctcttactgt
     7441 tgtgtgcact gtaatctcaa aaaatagcag tggatactat ttaattgatt gtggaagtaa
     7501 gtgccttgga cttgataaag gtgcacatgg aaatagtagt attgttggtt atggtagggt
     7561 atgtggacat ccagaactta ctattgattc actttcagaa gaagttggaa aaattaaagt
     7621 aaatggtgaa actgatataa aaattggtga tagaataaga attataccta atcattcttg
     7681 ttccactgca aacttgacaa gtaatttatt aggttttaga ggagatacaa tagaaaaagt
     7741 catagaagta gatattcgtg aaaattcaaa acaaatcatc ctttaaaaac acatttattc
     7801 tgatattaac atgaaagtat atttttctta aaattattgc taataactaa tgttaatgtt
     7861 ggataaataa aaaacataaa ttctagaaat ataaatttaa aagaagaggc tgtctcaaaa
     7921 tagagataag cctctctttc attgttgaaa ttatgaaaat atacttgat
//
LOCUS       DAYAOX010000164         7923 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.165, whole genome shotgun sequence.
ACCESSION   DAYAOX010000164 DAYAOX010000000
VERSION     DAYAOX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.165"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(146..901)
                     /gene="dapB"
                     /locus_tag="ACGI57_002808"
     CDS             complement(146..901)
                     /gene="dapB"
                     /locus_tag="ACGI57_002808"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFG9717876.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1341..2330
                     /locus_tag="ACGI57_002809"
     CDS             1341..2330
                     /locus_tag="ACGI57_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFG9717877.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2782..3498)
                     /gene="dapD"
                     /locus_tag="ACGI57_002810"
     CDS             complement(2782..3498)
                     /gene="dapD"
                     /locus_tag="ACGI57_002810"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFG9717878.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3722..4471)
                     /gene="dapB"
                     /locus_tag="ACGI57_002811"
     CDS             complement(3722..4471)
                     /gene="dapB"
                     /locus_tag="ACGI57_002811"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFG9717879.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4538..5425)
                     /gene="dapA"
                     /locus_tag="ACGI57_002812"
     CDS             complement(4538..5425)
                     /gene="dapA"
                     /locus_tag="ACGI57_002812"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFG9717880.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5542..6543)
                     /locus_tag="ACGI57_002813"
     CDS             complement(5542..6543)
                     /locus_tag="ACGI57_002813"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFG9717881.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     regulatory      complement(6716..6890)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(6968..7849)
                     /gene="dapA"
                     /locus_tag="ACGI57_002814"
     CDS             complement(6968..7849)
                     /gene="dapA"
                     /locus_tag="ACGI57_002814"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFG9717882.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
BASE COUNT         2700 a         1283 c         1030 g         2910 t
ORIGIN      
        1 ataagataat ctttaagaaa aatgattata tttaaaaggt aaacataaaa tttataaact
       61 tttagaatag aaatctcaat caattagata ttcaaattga tgaatatgaa tacctaaata
      121 gattgagatt tattatgtac aaaacttatc ctaatatatc tgacatttta taaagcccag
      181 attgcttacc aattaaaaat ttagctgcaa ctatagaacc gtttgcaaat acttcatcag
      241 attgagaatg atggcttaat gtaagaactt cattatcacc tgcaaataca acatcatggt
      301 cgctgattat attgccacct cttatagagt gtattccaat ttcatttggt tttcttttac
      361 aatctctacc atatctacca tgtgtatttt cactttcagg caagacttct ctaattgcat
      421 tagctatcat agtagcagtt ccactagggg cgtcttcttt tctattgtga tgtttttcta
      481 tgatttcaat atcaaaacct tctaacatct ttgaagcatc cttaactaat tttattaata
      541 tattcactcc aagagatgta tttgatgata ataatatagg aatgtttttt gatgcttctt
      601 ctattttatt taagtcattt tcatcatatc cagtagtagc taatactaca ggtgtattat
      661 tttttagagc atatgacaat attggctcta aagtctcatg atgtgaaaag tctattatta
      721 catcagtttt ttcagttaca tcagtcattg ttttgtaaag ctttgtatca ctatgatgac
      781 ctttttcatc agcagaccta gctacaccac aaaccaactc taattcatta tcttttttta
      841 cacattctgc taaaactctt cccattgttc cattatagcc actaattata actttcaaca
      901 taaattcctc ctatgaaatt aataaaaatt tgttatattt ctataagttt attttatcac
      961 taatataatt tatataaaat aatattagtg ataataagct tacaatttat cttacattaa
     1021 aaaatttaag tggatttttc aaaatataga taaatttctt tttcattgta taaatgataa
     1081 aaatatattt gacttcatac caaaaagagt gccccatgaa ctaaaaagtt aattttgaaa
     1141 taccatattc atatatcata tgatttattt ggaaatcaat attaatacat taaaataact
     1201 aaatacatgt atgcataaag atgtataaaa aatatataaa catatagtat taattttaaa
     1261 ttcaacaatt taattgttaa aagtgttatt attaaattag aaatatctta acttaatata
     1321 ctgagatggg agtgatagat atgttaaaag aagaaaaaac ttatcaagag aaagacgcaa
     1381 aagtattcaa tggagtggca gctttgataa tgattttatt atttttagta gtttcaatag
     1441 gtatcatggt ttttgggata gtaaaacttg atagtggatt tactggaata ggttcactta
     1501 tgttaatttt gggacttgta tttatagtag tagattttat cctatttttt gggctaaaaa
     1561 tgattaatcc taaagaagct atagttctag ttttgtttgg taactattat ggaactataa
     1621 aaaaagaagg gtactattgg gtgaatccat tttgttcagc aataaaccca gcagtatcaa
     1681 gaattaatgt atcgaaatct tcatctgatg aaaaggtaga tatatcaact aattcaaggg
     1741 gcaaaaaagt atctttaaaa acaatgactc ttaataatga aaagcagaaa gtcaatgatt
     1801 tattgggaaa tccaattata ataggagtgg ttgtaatttg gaaggttata gatgcaacaa
     1861 aggcagtatt taatgtagat aattataata cattcttatc tattcagtgt gattctacta
     1921 ttcgcaatgt ttcaagactt tatccatatg atgtatctga agatggagat gaaaaatctc
     1981 ttcgtggtag tagtcaggaa atagcagata gattaaaaga tgaattacaa tcaagggttg
     2041 atattgcagg aattgaagtg tgtgaagtga gaattactca tctttcttat gcaccagaga
     2101 ttgcagcagc aatgttacaa cgtcaacaag cagaggctat aatagcagca cgtaaaaaga
     2161 ttgttgaggg ggctgtaagt atggtagaga tggcattgac taatttaagt gaaaataatg
     2221 ttgtgacttt ggacgaagaa cgtaaagcag ctatggtaag taatctgtta gttgtgcttt
     2281 gtggcaataa agatgcacaa ccagtggtaa acagcagttc tatttattaa attataaaaa
     2341 gtttaatagt ttattttatt gatatataga aaatttagta gttcatttta ttaaattata
     2401 gaaagattaa tggtttgctt tattgaatta taaaaagttt aataatttat tctattgata
     2461 tatagaaaat ttagtagttc attttattaa attatataaa gattaatggt tttcacttta
     2521 ttaaattata aaaagtttaa tagtttatta cattgatata ttgaaaaatt gaaggaaata
     2581 aaaatacttg tcagcaagaa ataaaaatga tataagaatg aataagagaa ttgtaaaaaa
     2641 ataaaatgat tataaataaa agataaaaat attgtaaaaa agagggtgcc tctggtgaat
     2701 taaattcaca cagagacacc tttttaatta attttttaaa ttagtatatt attgtttaat
     2761 taatagtata gtactattta attaatctaa atttctaagg tcttccataa gcttagtttt
     2821 gtcagccgtt ttttcatctt tcatttttat aactttagca ggagaaccag caacaacggc
     2881 accagcttct acatcagtag ttacaacagc accagctgca acaacagcac ctttaccgat
     2941 tctaacacct tcaagaataa ctgcattagc tcctattaaa acatcatctt caactataac
     3001 aggagtagca gaaggtggct ctaaaacacc agcaacaaca gcaccagcac ctaagtgaac
     3061 atttttacca agagttcctc tagctccaat aacagcattc atatctacca tagaaccttc
     3121 accaataaca gctcctatat ttataacagc tcccatcatt acaacagcat ttttttctat
     3181 agtaaccatg tctcttatta ttgcacctgg ctcaattctt gcatgttcac ttaaagtatt
     3241 ttttagagga atagcagaat ttcttctatc atattctaca tgagtatctg ttatatcatt
     3301 tccatgttta tctaatactt ccattatatc ctcatagtca cctacaaata tataagaacc
     3361 attacttccg aataccttaa atttatcagt tgattggaag tgtaaagagt tagtatttac
     3421 atatacctta acaggtgttt tctttttaac ttcttttata taccttgcta tttcataagc
     3481 atcgtttaaa tttaccattt gttaattctc cttgtatttc attatatttc ttttattata
     3541 tacttatttg tcaattttag tatatactta cttcttatta tttatatttt attttagtta
     3601 gtttttattg tatagacata gtattaaata aatttattat atatgtttga tttgtattgc
     3661 gtattattat atctatattg catatgttta atttatattg tgtattatta tatttatgct
     3721 attatcccaa catgctgtcc atattataaa atcctggttt ttgcttaact aagtatttgg
     3781 cagcagctat agaaccactt gcaaagatac cacgagattg agcttgatgg cttattgtaa
     3841 gaacctcatt gtctccagca aatagagcat catgttcgcc aacaatattt ccacctctta
     3901 tagcatgaat accaacttca tttggttgtc tttttgcact acgaccatat ctaccataat
     3961 tattttcaac gtttggcaaa acctctttta tagcgttagc aatcataaca gctgtgccac
     4021 ttggggcatc tacttttctg ttgtgatgtt tttctattat ttctatatca aatccttcta
     4081 gtaatttagc tgcatcttta accaatttta ctaatacatt tacaccaaga gacatattat
     4141 atgattggaa tacaggaata actttagccg cttcacgtat tttgttcatt tcgtcatcat
     4201 tatatccagt agtagcaatt actaaaggtg ttttagtctt taaaacgtaa gataagatat
     4261 tatctaaatt agagtgatga gaaaaatcta taactacatc agcttcctca actatagtac
     4321 tcatatcttc ataaatttta aggtcgcctt catagctatc cagacctcta gctgcaacac
     4381 aaaccaattc taattcatta tcttctctta tagtatcggc taaaatctta cccatttttc
     4441 catcataacc agttgctata acttttaaca taatttcctc ctatgattaa gggctaactt
     4501 ttgaccaaac ttacttttta ccatgcatta atctttacta tgcattaact tttagcccaa
     4561 agttaactaa ttctttcttt aatacttcta aatttgctgg gtccatttct gctagaggta
     4621 atcttaaatc tccaacattg aatcccaata aattcattgc agtcttaact ggtataggat
     4681 ttacttctat aaataaagct gcgattagag catccatacc aagttgtaat tttctagaac
     4741 cttcaatatc gccttcaaag aatttagcta ccaaatcgtg agtttcttga gggcaaacat
     4801 tagcaagtac tgatataact ccttgacctc ctaaagataa taaaggaagt atagtatcat
     4861 cattaccaga gtagatagca aagtcatctg gaactaaact agctatttct gcaacctgtc
     4921 ctaggtctcc actagcttct tttacagcaa ctatgttatc aattttagca agttcaacta
     4981 taaggctagg acttatattt acacaagttc ttccagggac attgtaaagt ataattggaa
     5041 gtttaacact gttagctatt gtttcaaaat gacgcttaag tccagcttta ttagctttat
     5101 tgtaataagg tgttattatt aatagagcat ctactcctaa tttttcagct tcttgactta
     5161 agttaactga gtgcatagta tcattagaac cacttccagc tataacagga actcttttat
     5221 taactttttc aacaacatac ttaatagtag caagttgttc ttcatcagtc atagtattag
     5281 cttcaccagt agtaccacaa gatactatag catcagtacc attagctatt tggtattcaa
     5341 ctaattcacc taatttgtca aaatcaactt tgttatcctt tgtaaatggt gtaactagag
     5401 caacagcaga acctttaaat atcatataaa atcaatcccc tcttttttat ttttgtgttt
     5461 tatattacag ttacttctaa tgcttgtttt tgagttacta ttaatatttt acaaaattat
     5521 atatttatac aagtttgtat attatactaa atcgtatgat aaagccattt cagctatttg
     5581 aattgcgtta gttgcagcac cttttcttat attatcagca acaacccaaa gatttacacc
     5641 attatcaaca ctaaaatctc ttcttattct tccaacaaac acttcatctc tatcatcaaa
     5701 ttctacagca gttggatatt cgtttttagc aggattatca actactacaa cacctggagc
     5761 ttcttcaagt gtttttacta aatcatctaa ctcaaatgga ttgttaaact caacatttat
     5821 agattcacta tgaccatttt ttactggaac tcttacagta gttgcagtta tttttaaatc
     5881 gtagttatca agtattttca tagtttcatt taccattttc atttcttctt tagtgtatcc
     5941 attttcagtg aatgaatcta tatgaggaag acagttgtaa gagatttgat gagggtagaa
     6001 tccagtagga ttaccgttca ttcctttctc taaatcttca acacctttaa caccagaacc
     6061 agatacagct tggtaagtag agtaaacaac tctcttaatt ttgtatttat catcaagagc
     6121 tttaagggga accatagctt gtatagtaga gcagttagga ttagctatta tacccttatg
     6181 attttttatg gcttctggat taacctcagg aactactaaa ggcacatctg gattcattct
     6241 ccattgacta gagttatcta ctactaagat tccatttgaa gcagctattg gagcatactt
     6301 ttcactaata cttccaccag cagagaataa tgctatttgt atgcctctat cagtaaatga
     6361 agtttccttt aattcttcta ctatatattc tttaccacaa aacattactt tagaaccagc
     6421 tgatttagaa gaagagaata aaaatagatt ttctattgga aaatttcttt cttctaatac
     6481 ttttaaaaat gttctaccta ccattccagt agcaccaact attgctacat ttactttttt
     6541 catataaatt caccccaatt ttaaattatt aaaacattat tttattttag ttatacaagt
     6601 aagatatgtt aatattttta tatatccaaa aactttaata ttatattaac aactttaatt
     6661 atatacaaaa aacgtacaaa ggtatacttt gtacgtctaa tttatcactc aaagtaaacc
     6721 ctgtagctca tcacctacaa aaataagtga cagtattaca catgttatgt gcaatcccag
     6781 aaaatattat tgcaagaaat attttcttcg gctgtaaatc ctttttccaa tcattaacac
     6841 ctgccagcta ctattggata ctcttagtta cagctacctc taccctaggt taataggata
     6901 ttaaaatttg attattctaa taataataca tatttaagaa aaaatcaact tatttatata
     6961 aatattgcta aaatttaaat ccaaaatttg tcaattcttg ttttaacaca ttaagattag
     7021 tttcttccat ttcagctaga ggaagtctta aatcaccaac attaaaacct aaaatgttca
     7081 tggcagtttt aacaggaact ggatttactt ctataaataa agcagcaatt aaagcatcca
     7141 tatcaagttg taattttttt gagccttcaa tatcaccttc aaagaattta gttactaaat
     7201 catgtgtttc tttaggacat atgttagcaa gtacagagat aacaccactt ccacctaatg
     7261 ataatagtgg tagtatagta tcatcattac cggagtatat agcaaagtct ttaggtacta
     7321 atttagctat ttctgcaact tgagctaagt ctccacttgc ttccttcaca gcaactatat
     7381 tatctatttt agcaagttca gccacaacag atggctttat attcacttta gttcttcctg
     7441 gtacgttgta taaaataatt ggcaacttaa cgctatttgc tattgtttca aagtgaagtt
     7501 taagaccttt ctcattagtc ttattatagt aaggagttat tattaaaaga ccatccaccc
     7561 ctaatttttc agcttcttga ctcatatgaa cagacaacat agtattgttt gaaccagtac
     7621 ctgctataac aggtattctc ttattaactt tttcaactgt atattttatt acagcaaaaa
     7681 tttcagattc actcatagta gtcgcctcac ctgttgttcc acaaacaact agtgcatctg
     7741 tgccattctc aatgtgatat tcgattaatt cacctaattt ttcaaagttg acattattat
     7801 cctctgtaaa tggggtaact aaagcaacag cagaaccttt aaataacata taatcaaatc
     7861 tcctttttta tctaaattta attttttaag tacaataata attatagtac aaattaatta
     7921 att
//
LOCUS       DAYAOX010000165         7898 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.166, whole genome shotgun sequence.
ACCESSION   DAYAOX010000165 DAYAOX010000000
VERSION     DAYAOX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.166"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(237..362)
                     /locus_tag="ACGI57_002815"
     CDS             complement(237..362)
                     /locus_tag="ACGI57_002815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717883.1"
                     /translation="MRFEMVEVNIKIKGEVSEIINSIRGIFNSFDEELILDIKSE"
     gene            complement(670..888)
                     /locus_tag="ACGI57_002816"
     CDS             complement(670..888)
                     /locus_tag="ACGI57_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717884.1"
                     /translation="MKILQQFKPYKYDKEKNIEYDEKIIANQNTEVYIVYPRISDEEN
                     RRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(1167..1322)
                     /locus_tag="ACGI57_002817"
     CDS             complement(1167..1322)
                     /locus_tag="ACGI57_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFG9717885.1"
                     /translation="MALDVKIEVKIIKDVVILNITHWKKETSLLTVKIARSQGDLSYV
                     KYFANES"
     gene            2033..2257
                     /locus_tag="ACGI57_002818"
     CDS             2033..2257
                     /locus_tag="ACGI57_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9717886.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRD
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     repeat_region   2544..2836
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2609..2637
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
     gene            complement(3490..3642)
                     /locus_tag="ACGI57_002819"
     CDS             complement(3490..3642)
                     /locus_tag="ACGI57_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717887.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRTKSDLRVEFKF
                     IFKSKK"
     gene            complement(3792..5219)
                     /locus_tag="ACGI57_002820"
     CDS             complement(3792..5219)
                     /locus_tag="ACGI57_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HFG9717888.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISVNALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKIVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTVAKNNAIVLVDNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKVYN"
     gene            5410..6231
                     /locus_tag="ACGI57_002821"
     CDS             5410..6231
                     /locus_tag="ACGI57_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFG9717889.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            6356..7873
                     /locus_tag="ACGI57_002822"
     CDS             6356..7873
                     /locus_tag="ACGI57_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9717890.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKAYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
BASE COUNT         2923 a         1120 c          922 g         2933 t
ORIGIN      
        1 tatcgcctct tcaatttgag ccccatcatt atataaaatt aacctacttg atatcttaat
       61 ttaatatatg tacactattc atatcttaat agtcaatatt attcattttt aatagctctt
      121 tttagatgtc agaattatta tattttgaaa tttattaaat ttttgcatta aaaaaactag
      181 aaattaattt aagtcttgaa ttacttataa ctttctacgt atctaccttt atattcttac
      241 tctgatttta tatctaatat taattcttca tcaaagctat taaatatacc tctaattgag
      301 ttaattattt cacttacttc acctttaatt tttatattaa cttcgaccat ttcaaatctc
      361 atctttttat cttataattt ctataagttt ttttatttat tgtgttgcta ctttgaccag
      421 cactttttta tttaatagtc taaatgatat tttttaccag ttttaatatc cttaaatact
      481 atatttacta aaaacttata atttctttta atacatagtt aatatattct tattactaat
      541 aacaatactc aacaatttta tcgccttttt tcaaatatga tttaactttt ttattcctaa
      601 ttatttaaaa atagttgtcc aattttcttc tatgagaaag tgctaagtat tagcaacttc
      661 tctattctct taactttcta ctgtctcaac tttcttcata gctatattaa tagctacttc
      721 tttagctact tcattgaact cttcccatct tctcctattt tcttcatcag atatacgtgg
      781 atatactata taaacttctg tgttttgatt agctataatt ttttcatcat attctatatt
      841 tttttcttta tcatatttgt atggtttaaa ttgttgtagt attttcataa catcacccct
      901 tgttataaaa tatgaactca taaacttgtc ttatacttat ttaattgtta caatttttac
      961 ttaaaatcac tttatattct atattttaaa gctaaatcct ttacaaaagt aacatattct
     1021 tctataagtt tcttatcctc ttacatagta tctagtttgt ttaacttatc tcttttaaac
     1081 ttacatacgc cttcttctgc cattcgtctt ttattaattg ttaaccttgt ttcaagagac
     1141 acaccaaaac tcttgttaag aagctcttag ctctcattag caaaatattt tacataactt
     1201 aaatcacctt gacttctagc tattttaact gttaatagac ttgtttcttt tttccaatgt
     1261 gttatattta atataacaac atcttttatt attttaactt ctatttttac atctaatgcc
     1321 atattattgc tttattaatt tgtatcctta aatcttaaat tttctattaa tacatcttct
     1381 atacagctta gtttctctcg cttaatcttg aaatatattt tctctaaatt gtcaaattgt
     1441 tcccaagttt tatcaatatc taatattttg cagtgtctat ttgcacatct ttcgatccaa
     1501 aggtataatt gactagaatg ttttgaaatc aactgactat taatagatta gttcttaaat
     1561 tctttcaaat actcgaattg taataaaaca taatttacat tctctttatt tctattgaag
     1621 ttagtttata tattgtttac atcattttca tatatatctg ctagttgcta tgcatttaaa
     1681 attctcttat tatttcttta tattacttgt aaattattca tcctcatcat cctcttaatt
     1741 aaatttctat cttagaaatt taatttgtaa aaaaattttc tatatcataa tcaagtatct
     1801 tagatagttt ataggtaaca tatcaactct aattgaatat tcccctttcc tgtacttaaa
     1861 ataaattgat gcatttttaa aacctaactc atttgcaaca ctttgtaagt ataaatttta
     1921 tgtatgtctt cttttttata aaatcatagt gtatcttttt catatattca cattaattgc
     1981 tatatttcaa taacaatatt tccaaaacag aaacttttcg agataattat gtatggatgt
     2041 aaatcaaagg attaaatata ttagggaaaa tatgaatagt tctcaaaaag aattgcttga
     2101 aaaaacaaat ataaatacaa gtgttatgaa tagaatagag tctggtgaaa gaccaacaag
     2161 agacgatggg ttaataatat ttgctaaaat ttttggttta tatactgatt atattcttgg
     2221 tttatcagat acagaaaaac taaatattgg caattaaagc tgaattaatt gtattatatg
     2281 tgtgatgaat ataaatagag aattttttga aaagcttgaa ataacttaga aaaaataaga
     2341 ggttcactca agaacaagtt gctaaagatt aaaaaacaac aaaggtatct actaaaagat
     2401 atgaaaatag tttaagagag ttgagtctgg aaatatttat gtgtattctt taaataaaaa
     2461 tttatttttt aaatgataat taaattttta ctgatatggc tatgctttta aaagtcctaa
     2521 ccttattttg atattatatc tgtctttaca taccacttaa ttaatataaa acagtataaa
     2581 aattagttac agatgtatct tttttgttct ttacatacca cttagttaat ataaaactat
     2641 taattttcct atgcttgtac tcatactcaa aaagctttac ataccactta gttaatataa
     2701 aacgattgtt attttacgaa aggtacggtc gtaatttacg actttacata ccacttagtt
     2761 aatataaaac aagctagcgg ggatagacaa tataaatatc aacacttctt tacataccac
     2821 ttagttaata taaaacccca aaataaattg agcatttcca agacttacat ctatacaatt
     2881 ctctcaaatt tgcagtgaac cacgagtagt gcaaatgata atatttatca tataccctca
     2941 atgccttatg tttcaactgt taagccctat tttatcaaaa atatcgctca ctgcaaaact
     3001 atttataata ttatgaatat attattttta aatatctgta ccaatttgta gtataataaa
     3061 atcaagaaaa taatttactc ctataaagag tgattatttt taagtgaaaa tattattttt
     3121 ttaaatcatt cttattggcg tttgggagat ttttatttta tcataaatat aaactgatat
     3181 aacaccagct gacatgcttg ccactttaga ttattattct tgctacaact attataacat
     3241 ataaaactta cttattttta ccaaatattt tatgtaaaca ataaatatta acttatgaaa
     3301 tatctgctca tttacagata tttaataagt taatagctct ataataaaat ttttatcatt
     3361 gtttataaaa aattttactt tgcatttttt tataaacaac agttttattt ataatgtttt
     3421 gtggtataat aaaagcaaga agaactacaa tctattttgc gttagagtgg agttcatcat
     3481 aaacagagtt tattttttgg atttaaatat aaatttaaat tcaactcgta agtcactctt
     3541 tgtgcgagag tggctttttg cttttttgaa tagtttacta attaagtaaa ctaccacact
     3601 agctgttaaa ctagctaaaa cattaaacaa aaagttatcc atgaactcac ctcccttcga
     3661 atcgttggga ggataatctt ttgtacatga actccactct atagattgta agttaaatct
     3721 tcttgctaaa aatattataa catattatgg taaattatat aattaaaaac acttgtttaa
     3781 aagttctaat tttaattata aactttccca gtttctatat ctataaaaaa ccaaccaaaa
     3841 gtaccattac tattttttga attgatttta acagcataga cattaccact atatatatca
     3901 tctctaaaat ctatcatatc aactactaag ttattgatac tgactttatg tctcttagca
     3961 cattctttta atgctatctg ttttgcttgt tcctctgtta tacttccttt atttttataa
     4021 tcatctggat tacatacttt catattacca ttaacatcat atgtataaac tttgctcata
     4081 tcatttttag ctacacagta tctaacatca ccctctgcac cctgtttaat gtaagcaaat
     4141 acatagtact cttttcctga aaaatcaaaa tttttatcta gtagaaatat taaatcttca
     4201 tcttctgaat aattaggttc gtaataaaat ttcttcacac ctaatagttt tttcactgca
     4261 ttagcagcat cttctactgt atattctctt ccagtattag aatcgcctat attttttgtt
     4321 acatttaaac actgctgtaa tatactatca ctcaaatttc ctatagcagt taatttagtt
     4381 gtacttttca atacactttt attactatca ttatcaacta acacaatagc attattttta
     4441 gcaacagtag aaccaacaag agcatacact aaatcagtac cacttgctat ataaaattct
     4501 tttactccac tataaaattt atttattatc tttttgttag tatcatatct atctgtccca
     4561 cctaatctag ttgaattagt atccttaact attttgtcat tcattgatga agtaccacct
     4621 attacataac tttctacacc agttgtatta aatggaacac tttttccatc agttaatact
     4681 atagctgctt tatctctaac tgctacaggt gcagcactca ttgcatcagg ctctccttta
     4741 aatgcattag tcaatatgac tttatttact ttgttaattg agtttatttc ttttgcgaca
     4801 ttatagcttg tcttaattct atcactacct tgaagtcttt taatctctat tcctttgcct
     4861 tttagaacag tttcagtata tttatcaata gagttttctc ctccaattat atatactttt
     4921 tttgcctttt ctaatctttt aagtgtagca tttggtatgt tatttttctt agtcaaaaga
     4981 attggagcgt ttatagctcc tgcaagtcca cttgcactta atccatcagc cattgtactg
     5041 tctgcattaa ttaatatagc tgtagtatag ttttgtttgt ctgcaattat accagcagtt
     5101 tcgtatttat ctgcaccttt aattgattca attttatcaa gagcatttac agatataggg
     5161 caactagcaa taaatataga taaagataac cctattgtta ataatttttt aattttcata
     5221 atatctcctt ctcttaaata ttttcaccta aattatataa tacttttcag gaaaatttta
     5281 ccataaattt aaaaggttat tattacttaa tataatatta atgaaaaata tcactcttaa
     5341 attaatgact ttggtagaat atggtgataa aataaaatta atgagagaca atataagggg
     5401 gattatatta tgataatcag taaaaaaata acagcaatat tagtatcatt tttaattatg
     5461 atatcaatgg ttggatgtgc tccaaaagaa agtgctacag aagtagctaa aaagtacctt
     5521 gatagtatag aaatcaaaga ctacgaagta gcatataatt tattaagtaa agacagtaag
     5581 gaaagtgtca gtctagaaaa atttgaagaa tatcaagata ctttatttaa acttcaaacc
     5641 ctaaaagatt ataaacttgg tgaagaaaat aaagtagaga aatacaaata ttctaataaa
     5701 gagtacacaa atcttgttaa aataaaagaa actgatacta taaagtatat cactgaaaat
     5761 aaagaagata ctgcaaattt tgatagatat tttataatcg aagataatga atataaatta
     5821 ttatggcata aagactttaa aagagctctt agctctaatt atgtcaaaat cgctaggtca
     5881 gaaacagaaa aagatgaaga taaggattat aataaagctg ctattgatat caaaaaagct
     5941 atagaaattg atgataaaaa tccacttgca tattatacac aatcatatat ttatatagag
     6001 ttacaacgat atgatgaggc tttaaagtca attgataaat acttagaata tctagataaa
     6061 aataatgaag cttataaaat agacgctagt gatgcttaca atttaagagg tgttattttt
     6121 atgagtaaat cagaatttaa taaagctaga gaatcttaca ataaggcgct tgaacttaac
     6181 ccggataatg aatatgcaaa aactaatcta aatgatatta aaggattata aggcattaat
     6241 attatagaaa ataagtatgt caaaagttaa attaactttt gacatgctta ttttctatat
     6301 tttaaactct attgatttac tatataatag tgcataagga gtgtgattac ttattatgag
     6361 tgttgccata tacttaagaa aatcccgtgc tgatgaagag gcagaaaaac aaggcgaatt
     6421 tgaaacttta agtagacata aatcaaccct actaaaacta gcaaaagaac aaaacttaga
     6481 tgtaattgag ataaaagaag aattagtttc tggtgaaagt ataatacata gacccaaaat
     6541 gcttgaactt ctaaaagaag ttgaagaaaa taaatatgat gcagtcttag taatggactt
     6601 agacagactt ggtcgtggag acatgaaaga ccaaggtatc atattagaaa cattcaaaga
     6661 aagtaagacc aaaatcatca cacctagaaa aacatacgac ctaacagatg aatttgatga
     6721 ggagtattca gaatttgaag catttatggc aagaaaagaa ttaaaactta tatcaagaag
     6781 gatgcaaaga ggtagaataa aatcagttga agaagggaat ttcataggaa cctctgctcc
     6841 atttggatat gatgctgtaa caactggtag aaaagaaaga atacttgtac caaataaaga
     6901 tgctgatgta gttaggacta tctttgactt atatattaat gaagatatgg gttgtagtaa
     6961 aatatcaaaa tacctaaata acttaggcat taaaactgct acaggtgcta attggtacaa
     7021 ttctgcaatt acaaatataa taaaaaataa ggcatattgt ggctacattc agtggcaaaa
     7081 aaaggactac aaaaaatcta aaaatccaaa taaaataaaa acagttaaac taagacctaa
     7141 agatgaatgg attgaggcaa aaggaaagca tgaaccttta ataagtgaaa taacatggaa
     7201 aaaagctcaa aatatactca aaaaaaatgg tcatgtcagc tatggtaatc aaataaaaaa
     7261 tccacttgca ggaatagtga tttgcaaaaa ttgtggtaga ccattagtat atagaccata
     7321 tgcagaccac gactatataa tttgttatca tcctggttgc aataaaagtt ctcgatttga
     7381 atttattgaa gctgccattt taaaatcttt ggaagacact gtaaaaaaat atcaattaaa
     7441 agcatctgat atagacttag ataaaaataa taaagatagt aatatagaat ttcaaaaacg
     7501 agtcttaaag ggcttagaaa ctgaattaaa agaattaggc aagcaaaaaa ataagttata
     7561 tgatttacta gaacgaggaa tatatgatga agatacattt attgaaaggt ccaacaatat
     7621 aagttcaaga actgaagaaa ttaaagattc aattaagaca gtaaaaaata gattgagtac
     7681 tgttaaaaaa gacaattcta aaataataga agatattaaa actgtgttat cactttacca
     7741 tgattcagat tcccttggaa aaaataaact cctaaaatct gtaattgata aagcagttta
     7801 ttataagtct aaagaacaaa aactagattc ttttgaatta atggtccatt tgaaattaca
     7861 tgaagaccag taataccaat aacatataaa gagtagtg
//
LOCUS       DAYAOX010000166         7893 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.167, whole genome shotgun sequence.
ACCESSION   DAYAOX010000166 DAYAOX010000000
VERSION     DAYAOX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.167"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..384
                     /locus_tag="ACGI57_002823"
     CDS             <1..384
                     /locus_tag="ACGI57_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717891.1"
                     /translation="LPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIKKALD
                     RLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKKNGFK
                     LDDILNKFNDFDLSEDEIKEVYENN"
     gene            1054..2394
                     /locus_tag="ACGI57_002824"
     CDS             1054..2394
                     /locus_tag="ACGI57_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFG9717892.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
     gene            2868..4769
                     /gene="cooS"
                     /locus_tag="ACGI57_002825"
     CDS             2868..4769
                     /gene="cooS"
                     /locus_tag="ACGI57_002825"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HFG9717893.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            4762..5235
                     /locus_tag="ACGI57_002826"
     CDS             4762..5235
                     /locus_tag="ACGI57_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9717894.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            5210..6436
                     /locus_tag="ACGI57_002827"
     CDS             5210..6436
                     /locus_tag="ACGI57_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9717895.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRILKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(6575..7762)
                     /locus_tag="ACGI57_002828"
     CDS             complement(6575..7762)
                     /locus_tag="ACGI57_002828"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9717896.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRIVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
BASE COUNT         3044 a          807 c         1353 g         2689 t
ORIGIN      
        1 cttccaaaaa ttcataaatc tgactatata gtaaatagaa ttatgctatg gggaaaactt
       61 attagtaatc cttctaactt agatgtaaga gttatagcta agaatgattc tattattaaa
      121 aaagcacttg atagattaaa agaattaggt agcaatgaag aatatttatt aaatctgaaa
      181 agaggtgctt atatcatgaa taaaagtaga aattttaaag aagagattga aagagagaca
      241 gcaattagag ttgcaaaaga aaaagattat aaaataatta taatgttgaa aaaaaatggt
      301 tttaaattag atgacatatt aaataaattt aatgattttg acttaagtga agatgagata
      361 aaagaagtat atgaaaataa ttgatgataa ataaaaagtg aacttatata agtttgctga
      421 atcggagtct ggaaatattt atatgtattt tttaatgaat gaaaatttat tttaaaaaca
      481 ataatttaga agtacagtta attatatttt tatgatactg tacttctagt tttttctatt
      541 gtagatttat tatttactaa taaatctaca atagaaatgc ttttgttgta agaatcattt
      601 tatctaatga ttgtttttta taatagttaa atttccattt gtagtgttta aatataaatc
      661 tataacagca tttctataaa tgttataaat cactataatg attcaactca aagttactgc
      721 ttaaagaagc agagatttta tcaaggtata tcatggaaaa aaatatttta ttgtaatatt
      781 tataagattt tattatagaa ataatgtaaa aactttataa ataatatatt ttatacctag
      841 ttatagataa atttatatag ttattattaa aaaaagcaat aaaaagttta attcaattta
      901 gcaaatttag tgatataatt aaaaaagaaa agaaatatgg acttatttgg aataattttg
      961 acaatctatt tactacatgt gtagagtcgt atataattaa attatacata aaatataaaa
     1021 aaataattta gtaatacgtt aggagaaagt attatgataa agtcaagctt attttggaat
     1081 attttcattg cgatatcctt tatgatagaa tggtttcttt gtaagaaaat gctagattat
     1141 acaagtgaga aaaggatttc taacaaaaaa ataaactttt gtatggtagg tattattgtt
     1201 tttgctgaca taatatattt ttttgatgta cctccaaata taagagtaat tatatctatg
     1261 ataattacta ttgcatttta taaaatctca tatgatgtgg ggtttataaa gtctgtatta
     1321 atatccttaa tttattggat gttactaatc gccacagatg ctttgagtat atctttaaca
     1381 gtttggctta attctataaa tgacatgagt agtatgggga cgggagatat atacagaatt
     1441 gagtctataa tattgggaaa gatacttttg attgtgtctt tgcttttata tagagcctta
     1501 aagataaaac ttaatataga aataaataaa aggacattat tttatatagt tattcctata
     1561 gtcgctaata tagcatcttt ttttgtaatt tttgaataca tatttaatcc agggtctggt
     1621 agcttaatac ataatttcca attcgttatg atttctgttg tattgcttat atcaagtatc
     1681 actttagttt taattgctaa aaaattacag caatataata ctttagttgt agaaaacaat
     1741 atgattaaaa atactttgaa gtatgaaaat gagtattatg aagatataga aaagaaatat
     1801 ttaaaaacta gattactaag tcatgatatg aaaaatcaca tattgtgtat aaatgctatg
     1861 attaaaaaag gtattgatgt tacttcttat ttaaataatt tgcaggtaga aattcagagt
     1921 aatgacttgt tatttaatac tggtaatttt atattagatg ctatattaag tgaaaagaaa
     1981 gaggtttgtg ataagaatga tatagaacta aaaataggaa taaattttag ttgttgtgaa
     2041 gctgttgaat tagtggatat atgtcatata ttttcaaata ttttagacaa tgcaattgag
     2101 gcatgtaata aaattgatag tggaaataga gttatttgtg ttaaaggtga tgttatgcaa
     2161 aatttttatt taataagagt tgaaaatact aagataaata agattattac taaaaatgga
     2221 gatattttga cagacaaaaa aggttctctt catggacttg gaataaaaag tgtaaaaaac
     2281 tctgtaaata agtatggtgg taatgttgta attgaatatg atgataaaaa atttgtagtt
     2341 aaaattgcta ttccacttag aaatgtgaag aaagcagtta atcatgtgat ttaaatgtat
     2401 ataaatttga tgtgtagaaa tttaatatat aagaattaat gtataaaagt gatgcataga
     2461 agtttaatat ataagaatta atgtataaaa gtgatgcata gaagtttaat atataagaac
     2521 taatgtataa aagtgatgca taaaagttta atatataaga atttaatatg tataaaagtg
     2581 atgcataaaa gtttaatata taggaattta atatgtataa aagtgatgca tagaaattta
     2641 atatataaga atttaatatg tataaatgtg gtgcatagaa gtttaatgta aaaatttggt
     2701 gtataagaat ttaatatata tatatttatt ttaataattt atattttgtt tagggtatgg
     2761 taaatcgtgg tattaaataa gtagatttta atagttaaat tatagtcaat tataataaag
     2821 ctatttaatc tactttttta atataaaaat ataaggggga atttgtaatg gcttgtaaca
     2881 cttgtaaaat gtgcgaaagt gcagacaaga aattagaaag ttttgtagct tcaaaggatg
     2941 ttgaaacagc atttcataga acagaagatc agaaagttaa gtgtggtttt ggacttcagg
     3001 gggtttgttg tagactctgt tcaaatggac catgtagagt aactcctaaa tcaccaagag
     3061 gtatatgtgg agcagatgca gacactatag tagcaagaaa ctttttaaga gcagtagcat
     3121 caggagcagc ttgttattta catgtggtag aaaatacagc taagaattta aaaaatgttg
     3181 gaataactaa aggtgttgtt aaaggagaaa aaacattaaa tcaattagca gagatgtttg
     3241 gaatagaaag tgatgaaaag tatgataaat gtataaaggt tgcagacaaa gttatcaacg
     3301 atttgtataa atcaagagat gataaaatgg aattagtaga aaaaatagct tatgcaccta
     3361 gagtgaaaaa gtggaaagag ttaggtataa tgcctggtgg tgcaaagtca gaagtttttg
     3421 atgcaatagt aaaatcatca acaaatttaa atagtgaccc agtagacatg ctagttaatt
     3481 gtttgaactt aggtatatca acaggattat atggtcttac actaactaat cttttaaatg
     3541 atgttatgtt gggagaacca gttataagaa tggctcctgt aggatttaat gtaatagacc
     3601 cagactacat aaatataatg ataacaggcc atcaacattc aacatttgca aatttccaag
     3661 atagattaaa agatgaagat gttataaaat tagctcaatc tgttggtgca aaaggcttta
     3721 agctagtagg atgtacatgt gttggtcaag atttacaatt aagaggagaa cattatcaag
     3781 aagtatttgc tggacatgca ggtaacaatt ttacaagtga ggctgtttta tcaactggag
     3841 caatagatat agttctttca gaatttaact gtactatacc agggcttgaa ccaatagcag
     3901 ataaatataa agtaaaaatg gtttgtttag atgatgttgc taaaaaagcg aatgcagaat
     3961 acataggttt agacagaagt aaattagatg aacttagtaa cactttaata gaaaaagcat
     4021 tggagtctta taaggagaga agaggaagta ttgaaataga tataccaaaa gaccatggat
     4081 ttgaacaatc tttaactggt gtaagtgaaa agaacctgaa agcgttttta ggagattcat
     4141 ggaaacctct tattaattta attgcagaag gaaaaattaa aggtgttgca gctgttgtag
     4201 gatgttcaaa tatgacagca ggaggacatg atgtaaatac tgttgaactt actaaagaat
     4261 tgattaaaaa agatataata gtactatctg caggatgttc tactggagga ttagaaaatg
     4321 ttggtcttat gtcacctgga gcagaagaat tagcaggaga aaacttaaaa gaagtatgta
     4381 agactttagg aataccacca gtattaaact ttggaccatg tctagctata ggaagactag
     4441 aaatagtagc aacagaatta gcagctgaac ttggaattga cctaccacaa ttaccattag
     4501 tgctttcagc tccacaatgg ttggaagaac aagcattagc tgatggagcg tttggtcttg
     4561 cccttggctt acctcttcat ttagctttac ctccatttat aacaggagga aaattggtga
     4621 cagaagtatt aacagaaaaa ttaaaagact taacaggagg acatgttata gtgaatccag
     4681 accctaaatc ttcagctaat caattagaag aaataataat agatagaaga aaaaatttag
     4741 ggttaaagga tgtggaatgc aatgcataga atttttataa ataaagattt atgtacagga
     4801 tgtaagagct gtgtactagc atgtatgcta aaacacaata aagattatga catgtacact
     4861 cttgatttag aaaatataga taatgatagt agagggcata tagaattaga tagtaaacat
     4921 aataatccag ttccaattct ttgtagacac tgtgatgaac ctgaatgtgt attagcatgt
     4981 atgagtggtg caatgcataa agatagtgaa agtggaatcg tttcatatga tgaagagaaa
     5041 tgtggttcat gttatatgtg tgtaatgtct tgtccatatg gattattaaa gccagatgat
     5101 agaagcaagc agaacatact taaatgtgat ttatgtaaag atgaggagta tcctagatgt
     5161 gtagctaatt gcccaagtgg agcaattgaa ttacagaagg aggaaaatga tgaattatgt
     5221 agtgttaggg gctagtgcag ctggtataaa tgcagtaaaa acattaagag aattagataa
     5281 agattcaaat atagtagtaa tttctaaaga tgaaaacgta tattcaagat gtatgttgca
     5341 tcacgttata tctgaacaca gaattttaaa acaaataaat tttgttgatg aagattttat
     5401 ggagcaaaat aatgtaaaat ggattgcagg taagactgtt aaaggtatag acataaataa
     5461 aaaggtagtt cagacagaag acattactgt aaattatgat aaacttttaa tagctacagg
     5521 tgcatcttct gctattcctc ctataaaaaa tttaagagat ggaaattttg tatattctgt
     5581 gagaaacata gatgatattt ataaaataaa agaaaaagca caaaattcta aaaatgtagt
     5641 tataataggt gctggtttag ttggtataga tgcattagtg ggattattta aatatgagca
     5701 gttaaatatt tctgtagctt ttatggaaaa gtacatttta gatagacaat tggatgaata
     5761 tacagcatca gtgtatgaaa ataagttcaa agaaaaaggt gtaaaatttt acccaagtgc
     5821 atcaatacaa gaaattgtat tggataacag taaaaatgtt acaggtgttg cattttcaaa
     5881 tggtgaagtt ttagatgcag acatggtaat tgttgctaca ggggttaaac caaatgctga
     5941 ctttttagat ggaacaggaa tagaatatga tagaggtata ataatagatg atatgtgcca
     6001 aacaacacaa aaagacatat atgcagcagg agatgttgta ggtaaaaatg ctatttggcc
     6061 tctggcagta aagcaaggaa ttgtagctgc ctataatatg gttggaaaag ataagaagat
     6121 agaagatgaa tttgctttta aaaacagtat gaatttcatg gatataccaa ctatttctat
     6181 aggaatgaat acacctgtag atgatagcta taaagtgcta actagacatg gaattaatga
     6241 ttataaaaag tttgttttta aagataatgt aatctatgga gcagttatac aaggagatat
     6301 ttcatatgta ggtgtattaa cttatttaat aaaaaataaa gttgaaatat atgatttaga
     6361 aaataggata tttgatatat gttatgcaga cttttttaat ataaaagaaa atggagaatt
     6421 ttgttatagt gtatagtata tacgtggagt aaggtttata aatacagtaa atcaataaat
     6481 agagttattc taaaataaga aaaatgacaa atcttattta gaaatagctc tattttttgt
     6541 aattaaattt aaattatatt caatttgtaa aatattattt tgaacaatta tacatatact
     6601 ctctagttag aggtaaatca ttttttatac ctttagtgaa aagtatttga tgtatatcta
     6661 taataccagt gttgaaagca gcagcacaag aacaaaggta taaatcccac attcttataa
     6721 acttatcatc aaacatttct ttaatctttg gaagattctc agagaaattt ttttgccagt
     6781 atagtaaggt ttttacataa tgtaatctta gactttctac gtctatagta tgatatttgt
     6841 aatcactaca aatatgtatc atttctctta ggctaggaat aactcctcct gggaaaatat
     6901 atttttttat ccaagggtct ccataagatt cctgcaatcc acttatatag tgaagtaaaa
     6961 acaatccacc atcttttaaa acagcattta cgtttttaat gaaaagttca tagttttctc
     7021 taccaacatg ttcaagcatc ccaacgctta caattctatc aaacataagg tctgattttt
     7081 ttaattctct atagtccata agtcttactt caagatagtc ttgaaggttc tcttctttaa
     7141 ttcttgcgtt aaattcctta aattgctctt cgctaagtgt aattccaacg ccgtgaattt
     7201 tatatttttt tgcagcttct attagtaaaa atccccaacc acaaccaata tcgagaaggc
     7261 tcataccttc ctctaaattt agtttagtta atatgtggtg aactttattg acttgagcat
     7321 cataaagagt atcattatca tttctaaagt atgcacaaga ataactaagt gtcttatcta
     7381 accataagct ataaaaatcg ttgccaatgt catagtgggc atgtatttct ttcttttgat
     7441 gtctcattga atttgatgag tgcaatagat gttttacagc tgaattatca atattgaatt
     7501 tgtctatttg ttttaaaagt agatttaaag ttaagaataa atctccctca acttcaatgt
     7561 ctctatttat gtaggcttct ccaagtgcta aagatgtact ttttaatagt tcagacttgc
     7621 taatatcatt atttactata actttgaact gaggggagcc ctctccaatt agaaattctt
     7681 cctcatcttt aagcctaact agaaatggtg tttcaccaaa tttctctaaa tagtctttta
     7741 aaaaattttt tgtaatattc atattaccag ctccttctaa tgtaaatcca gtaagtttcc
     7801 ctgcttaaaa tatattatag tccttttttt aaaaaagtaa aattttttca ataaattttt
     7861 tatttaaagg aaatttattt tttataaaga aat
//
LOCUS       DAYAOX010000167         7844 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.168, whole genome shotgun sequence.
ACCESSION   DAYAOX010000167 DAYAOX010000000
VERSION     DAYAOX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.168"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            962..2320
                     /locus_tag="ACGI57_002829"
     CDS             962..2320
                     /locus_tag="ACGI57_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFG9717897.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2427..3221
                     /locus_tag="ACGI57_002830"
     CDS             2427..3221
                     /locus_tag="ACGI57_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFG9717898.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3214..5520
                     /locus_tag="ACGI57_002831"
     CDS             3214..5520
                     /locus_tag="ACGI57_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFG9717899.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKILSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5659..6936
                     /locus_tag="ACGI57_002832"
     CDS             5659..6936
                     /locus_tag="ACGI57_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFG9717900.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASFNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7119..7841
                     /gene="yqeC"
                     /locus_tag="ACGI57_002833"
     CDS             7119..7841
                     /gene="yqeC"
                     /locus_tag="ACGI57_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFG9717901.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRNKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHRTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
BASE COUNT         3092 a          842 c         1462 g         2448 t
ORIGIN      
        1 agatagaaac ttgtatattt tatgttgcag atacaaatat agtaaaggag agtacattgg
       61 tacaactgag atacatagct tattaagttt ttctgtctta tgagaattat attataaaat
      121 attcataaat attttgagtt taaattaaat aattatttta tgcttagatt aattgatatt
      181 taattaattt aataacaaaa acagtgaaag agtaaataat attatttaca gggggtaaag
      241 caaagtatgt taaataaatt gtacttttgg ccccgtacat agagaaatct atgtaggaca
      301 caactttaaa accagtaaat cctaaagcct tgcaactaca ataaaagata gaaagaaact
      361 tttatgaatg tggtgaaaac cagaaacaat agtgaggatg gtatatggtg agagcctaag
      421 tacttaaaaa tggacatttg gtcgccaagc ctagaaatag gaaggtcaaa cgactatctc
      481 gagagagagt acactataag cgattgatag tggaaacggg ttgctcttag caggtaatgc
      541 tgaagatgaa gaaatagtct acgctcatat gaaagtatga ggtcactgga tttgaagtga
      601 catctataaa gtagcgttta taggtaaatg tttaaaactt gaaacatgta aatacttatg
      661 aaaacttata acttataatg attaagattg aaagatttaa attgaattaa atttactata
      721 aaaaattaaa aattcatagc tataatactc ttatatttgt aaagcatggt atataatgta
      781 tacaaaatta aagaatacgt gtatttgtta ataattaaat ttattgcatc tgttttttaa
      841 gactaaaaac ttaaatatat gttggagtat agttgatatt tatgaacatc attaatttaa
      901 tatattagat ttttaagtat ttatataatt caataagttt tatgtaacgg gagtttttat
      961 tatgaaagca aaaagtacat caaaatttga attagacgga ataccaccac taaaggaagc
     1021 aataccttta gggttacaac acttaatggc aatggtaata ggtagttgtt taccagcgat
     1081 attgattgca aatgcagctg gacttaatca taagatgtca acattattga ttcaagccgc
     1141 attagttttt gcaggattat caactttatt acaattatat ccaatacctt tgtttaaagg
     1201 ttttaaatta ggaggaggaa taccagttat aatgggagct acagctatgt ttataggagc
     1261 tggggtatct gttgcaagta agtatggact accagtatta tttggctctc aaataatagc
     1321 tggtatagta ataattataa ttggttttgg tcttaaaaaa atacgattta tatttactcc
     1381 agctgtatca ggaacaatag ttgcatgtat gggtcttgga ctttttccaa tagctataaa
     1441 aaacttagct gggggtatgg gcaatgagac ttttgggaat ccaattaatt ttatagttgg
     1501 aatatctgta tctcttatga tattaggatt aaataagtat ggaaaaggat tatttaaaga
     1561 tgcatcaata ttagttagca taatttttgg atacatttta tcattaattt tgggtatagt
     1621 aaatttttct tcaattcagg agtttacttt ggttgcactt ccaaaaccac ttgcttttgg
     1681 gctagatata agacttgaag ttgttgtaat gttttcaata atatatttag ttgagatagc
     1741 tgatataatg ggtgcttgta cactttctgc ggtaggaggg cttaatagac aagtaacaga
     1801 tgaagaactt tcttctgctg ttttaggaaa tggagtactc tctacaatag gtgcattatt
     1861 ttctgcaact ccaatgggga tgtttggaca aaattctgca atcgtttcca atacaaaggt
     1921 tgtaagtaaa tttgtactag ctattggagg aattggatta tttttggcag gaatatcgcc
     1981 tttattagct aatttaataa gaacaattcc tccctgtgtg gttggaggag caacactcgt
     2041 tatattttca actcttacaa catctggttt aagacttgtt agtatggatg gatttaatca
     2101 agaaaatagc atgatattag gtctttctat ggcttctggt attggattca tggtagctcc
     2161 tcaagttctt gaaaaatttc caaaatttat agagacatta cttgcagatt caagtgtagt
     2221 ttcaggagca atggtagcca taataattca agctttatat atggtaaaat ttcctggaaa
     2281 taaaagtgat aaggttgagg ataaaaataa aagtttataa tgtaaaggtt taatatatgt
     2341 gaattaaata tactacaatt ataaatataa ataaaagatt tattaaaaac ttttgtgttt
     2401 taataagtta atgatggggg gataagatgg ttactataaa ggaatattca gttccacaga
     2461 gtttagaaga agcatataaa attttaattt ctaaaaaaaa taatgttatt ttaggtggat
     2521 gtggatttat aaaattaagt aataaaaata taggaacagc gatagattta aaagatataa
     2581 atctaaatta tataaaagag gataaaaaaa atattttgat aggtgcagat acttcactta
     2641 gaagtttaga aattgataaa actataaaaa attactgtag tggaatactt tcaagcgctg
     2701 tttctaatat agttggtgtt caatttagac aaggggctag aattggtgca agtgtatttt
     2761 caaagtatgg cttttctgac ttaataccag cacttttggt tgttggagcg aaggtgagat
     2821 tatacaataa aggaatacta gagttaagtg agtttttgga tagtgaatta agtagggata
     2881 tattggtcga agtaatacta ccaaaaaaag atgcaatagc tgtttttgac tcaataagaa
     2941 aatgcacagg agatttttct gtattaaatt cagcaatgct aaaagaaaat gatacttaca
     3001 aaatagcaat tggggcaaga cctcaaaaag ctagattagc acttgaagcg agtaatatat
     3061 taaataaaga aaaagatata gataaggcat ctattgttgc tagtaaggaa cttacttatg
     3121 gaagtaatat gagagctagt aaagagtaca gaaaggatat ggcaagtgca ttagttaaaa
     3181 ggatgtataa tgctattgag gagggaatgt ataatgatta ataaggggat aagaaagata
     3241 gatagtacag caattgtcac aggaaaacct ctatatactg aagacctcat tatgcataaa
     3301 gatgttttga cattaaaact acttagaagt ccacatgctc atgctaagat aaagaatata
     3361 gatacatcaa aagccgaaaa aattccaggg gttgtgggta tttatacgta taaagatgta
     3421 ccacaggtca gatattgtgt tccaggtcaa acagaaccag aagcatcacc ttatgataga
     3481 gttttacttg aagatacagt tagatttatt ggagatgaag tagctttagt ggctgctgaa
     3541 gatgagaaaa cagcacttaa agccttaaaa atgataaagg tagattatga ggttttaaag
     3601 cctgtgtttg atgcaagaga agcagaagga aatgaagttg ttatacatcc agaaaaagac
     3661 atagtagagc catacccttt tggattggat gcaaaaaata atatagtatc aaaagaattt
     3721 tttgaatttg gagatgttga agctaagctt aaagaaagtg attttgtggt tgaatcttca
     3781 tacaaaaccc aaagtcaatc gcattgcatg atggaaactc ataggtcata ttcatataaa
     3841 gatataaatg atagaattgt tgtagtttca tcattacaat caccacacaa tgtaaagaga
     3901 atacttagaa aagtattggg tataccgtct tcaaaaataa gagtaattaa acctagaata
     3961 ggtggtggat ttggaggaaa gaatatagca gcaacagata tatttactgc atttgttact
     4021 atgaaaactg gtagaccagc aaaatttgta tttacaagaa gtgaaacttt tgtaggttct
     4081 aatagtagac atgaaatgtt ttttgatgtt aaagttggtg cagataaaga aggaaattta
     4141 aaagcaatag agctaaagtc attaaacaat acaggtgctt atggtgggaa tggtgtctct
     4201 gtttcttccg aatctggaca caatacctta ccaatatata atgatattga tgctgttaga
     4261 tttaatgcta gaacagtata tactaatagg ttaccagcag gagcacttag agggtatggt
     4321 gctacacagg gaatatttgc aatagatagt gctattgatg aattggcaca taagattgga
     4381 atggacccac ttgaatttag aattaaaaat ataatagata ggtataccaa tggaaaagca
     4441 gctgaagacg aaatattaag ttgtgagctg agacaatgta tagaaaaggg aaagaaatta
     4501 attaattggg atgataaata tccatgtaaa gatataggaa acaacaaagt aagagcagta
     4561 ggtatggcag tagcaaatca tggttctggt ataccgcgtg tagatatggc aacagtcact
     4621 ataagaatgg atgaagatgg cacatataaa atattatctg gtcttgctga cttaggtcaa
     4681 ggggctgata caattcaagc tcaaattgct gcggaagctt taaatactac tgtagataga
     4741 atttctattt atacaggtga ttcagactta tgtccctatg atacaggagc atttgcttct
     4801 tgtacgacaa cggttacagg aaatgcaaca ataaagacag caaataaatt aaaagatata
     4861 ttactttcag ttgcagagtc aaagctagga tttaaaaaag aagatttaga gttgggaaaa
     4921 gacagggttt ttgttaaaaa cgataatagc aagtttgttc tattagagga attgggtaga
     4981 gaatctgttg gaggaatagg atttgctagt gatgatgtac cagtagtttt ggaagcaaca
     5041 gaatcttttg gcatgttgaa aaaaataaaa ccatttatag caggatttgc agagatagaa
     5101 ttagataaaa atacaggtga atataaagtc ataaattttg catgtgtacc tgattgtgga
     5161 acagttataa atccaacttt agctagaata caagtagaag gtggtgtgat gatgggtata
     5221 gggtttgcaa tgtatgaaga ccctatattt gataaagatg gtagattatt aacagatagt
     5281 ttcctacagt ataaagttcc tacaaaaaaa gaagttggaa atatattggt agattttgct
     5341 gagaatttcg acccaggagg tccttatggt gctaaatcat taggagaaat agttatacat
     5401 acaccagcac cagctatagc aaatgcaata tttaatgcta caggagttcg tttaagagag
     5461 cttcctatga cttttgaaaa agtttatatg ggtatgaaaa ataataataa acaattataa
     5521 taaataataa gatgcataaa agcaagtaaa tttaaattat tttgtaaaat aaagtaatta
     5581 aatggacatt atatatggtg ttttgtaaaa ataccgtata taatgtctag tagagtatat
     5641 tctaaaggag gtgagttcat gtcaaacaga ataaaaaata tacactatgg aactcagaga
     5701 caacaattaa aaaacttaac taaaaaagaa tatcaagctc tacgagagat gtgcttctta
     5761 gctaagaact tatataatgt tggtacatat aatgtaagac agcattattt caatcaagga
     5821 gaacatctta actatgaagc taattatcat gtatgcaaag aaaatgagaa ctataagctt
     5881 ctcaatagta atatagcgca acaaatatta aaagaagtag acagttcgtt taggtcattt
     5941 tttggattaa ttaaattagc caaagaagga aaatatgact ttagagggat aaaatcacct
     6001 aaatatttag ataaagaagg attttttagc ataataatag gtcaaataag aatcaaagaa
     6061 gatgggattt taaatgtacc tatgtctcca gcatttaaaa aactatatgg gaaagtaagt
     6121 ataaaagttc cacaaaatat actagagaaa aaaattaaag aaataagaat aatacctaaa
     6181 cataaagcta ggttctttga aattcagtat tgttatgaaa taccaaaatc agaggaagtt
     6241 tcaaatcaaa aaaatgcact agctatagac ttgggtttag aaaatttatg tacatgtgtt
     6301 accaatttag gtgatagttt tatcatagat ggaaaaaggc taaaatctgt gaatcagtgg
     6361 gcaaacaaac agaatgataa aatagaagcc ttaaaattaa aaaagaatat aactgcaata
     6421 acaagaaaac aatttaaagt ttggaataag agaaataatc aagttaaaga ctatgtaaat
     6481 aaaacctgtt tttatataat tgattattgt gtaaaaaata atattggaaa tttaattgta
     6541 ggatataatg ctaagttaga aaaaagtgat aatatggaga gaaaaatata tagaaatttt
     6601 ataagaatcc catttggaga gataaaagga aaattagaat atctgtgtaa aataaaaaat
     6661 atcaatttta ttaggcaaga agaaagctat actagtaaat cagatttttt tgctgatgat
     6721 tttctacctg atatcaatgt ggacaatgtt aaaaaatatg actttaaagg ggaaagaata
     6781 acacgtggac agtacaaatc cagtgttgga atcattataa atgcagatgt aaatgcgagt
     6841 tttaatatac taaaaaaatc acaagtagtt aatttatcta atttacagaa taatcctcaa
     6901 atattaaagc aaccacagag agtaagaata tcttaattat atttaaaaga taaactgata
     6961 gcgtaagctt acagcttagc ggcgaattgg atccgccttg ttgtttacaa cagaatctat
     7021 ttactttcga gtttatggaa tttcaaaaat cacttttact aaaaaggcct aactataata
     7081 attaagagat tagaaaactg gttataagag gtgaaagtgt ggaactatca aatataatag
     7141 aaaagaatga aataataact gtagttggag caggaggaaa aacttcgttt ataaactatt
     7201 ttgcaaattt ttatagaaat aaattgaaag ttcttcttac aacaacaact aaaatatatg
     7261 taccaaacga ttatgacaat ataattatta caatagatgg aactgttatt ccatctattt
     7321 gtcatggaat aacagtctgt ggcagttata taaataatga aaataaatta gtaagtattg
     7381 attcctctat tttggatgaa attgtagacc aatttgattt agttttaata gagggagatg
     7441 gctcaaagag aaaaaaactt aaaggttgga atgctaaaga accagtagta tatcatagaa
     7501 ctacaaaaac catagggata ttagatataa catcatttgg aatgaatatt aatgaagaga
     7561 atatacatag agtagaaata tttaaaaaaa tagcaaattt agatacaagt agtattaata
     7621 gtagttctac agtaagtatt gaaaacttaa aaaatatagt attaaatcca aatggattat
     7681 ttaaaaatta ttctggaaaa agagtattat ttattaataa agttgaaaat gaaaaatata
     7741 aaaatttagc tataaaatta atagaaaata ttaaagaata tgaaagtgaa atagagatat
     7801 tttatggaag tgtaaaacaa aaattttgtg taagatattg aagt
//
LOCUS       DAYAOX010000168         7818 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.169, whole genome shotgun sequence.
ACCESSION   DAYAOX010000168 DAYAOX010000000
VERSION     DAYAOX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.169"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     misc_binding    81..292
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            455..1744
                     /locus_tag="ACGI57_002834"
     CDS             455..1744
                     /locus_tag="ACGI57_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFG9717902.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1798..2877
                     /locus_tag="ACGI57_002835"
     CDS             1798..2877
                     /locus_tag="ACGI57_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HFG9717903.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2898..4442
                     /locus_tag="ACGI57_002836"
     CDS             2898..4442
                     /locus_tag="ACGI57_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HFG9717904.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4605..5147)
                     /locus_tag="ACGI57_002837"
     CDS             complement(4605..5147)
                     /locus_tag="ACGI57_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HFG9717905.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5295..5579)
                     /locus_tag="ACGI57_002838"
     CDS             complement(5295..5579)
                     /locus_tag="ACGI57_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFG9717906.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5642..6406)
                     /locus_tag="ACGI57_002839"
     CDS             complement(5642..6406)
                     /locus_tag="ACGI57_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFG9717907.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(6688..6954)
                     /locus_tag="ACGI57_002840"
     CDS             complement(6688..6954)
                     /locus_tag="ACGI57_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFG9717908.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(6996..>7818)
                     /locus_tag="ACGI57_002841"
     CDS             complement(6996..>7818)
                     /locus_tag="ACGI57_002841"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9717909.1"
                     /translation="EDILMNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSI
                     KENLTLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVY
                     YLTGLSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNI
                     VDMTIPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLV
                     LQEIQNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         2714 a          997 c         1280 g         2827 t
ORIGIN      
        1 caagtaattg actttttaag ttgacaaaat attgcatata tattatgata taaatcataa
       61 attaactaca aatcaatatt atatattcgt agaagaagaa agtagcataa aggaatttta
      121 cagagagtta gatttggctg agaactaata ttggaattta tgtgaaaagg actttggaga
      181 atactattgt aattatgtta gaatagtggt ttaattcgcc ttaagaaaat gagtgagtaa
      241 aattaatttt tactaataag agtggtaaca cggatttata ttcgtctctt ggatttttcc
      301 aagaggcgtt ttttattata aacttatttc agaaaattta aaactatata ttaaatgaaa
      361 attataaaaa tatgatagtt aataaattta taaattagaa aattgtaaaa tatgctaagg
      421 tcagtagttt tacgatttaa atggagggat gaaaatggaa gaagaaaaaa ttaactatgc
      481 tctggaacag attccagaga atcaaaaacg tggttgggta gctatgtttt cagttttagt
      541 agcaataggt gtggatttat catctgttat tttaggcgct gagttggcac agagtatgcc
      601 aatgaaacaa gcaatattgt cagtaattgt tggttcattt ttttctgcaa ttttgtatac
      661 tacatgctct ttggttggtt catctacaag tttatctaca tctatgataa caaagtatgt
      721 atttggagaa gctggagcaa aaatattttc tctagttata ggggtttctc tattaggttg
      781 gtttggagtt caagtaggtt tctttgctca aaatgcacag ataataataa aagatatatt
      841 taatttagat gtcagtatgc agattttaag cctaataggt ggtctcttga tgatgagtac
      901 tgcaatttat ggatataagg caatggagaa gttgagtgtg tattcagtac catttttatt
      961 agttcttatg atgctaacta tattcttagc ttttagagca aatggaattt cagttgatga
     1021 taatatgaaa tctactatga catttgcagg tggagtttct ctgtcaatga gtataataat
     1081 agttggtgca attgtatctc ctgatataag tcgttgggct aaaagtagaa gagactgtgc
     1141 tctttcaagc tttttgggaa tacaatttgg aaatgctttt atgataatag tttcgatagt
     1201 attagtaaaa tgtatgggaa cttcagatat tatgagaatt tttattacat taggaattgc
     1261 tataccaggt ataatagtac ttacattagc acaatggact acaaatacaa gtaatgtata
     1321 ttcagcatca ttaagtatag ctcttgtact taagaaggct cctgagaaag tattaactat
     1381 agtccttggt attatagcaa cattattggc tgtatttgga atatatgaag gttttatagg
     1441 atttttaaat ttacttggaa ttgtaattgc acctgttggt ggtgtatata cagcagaata
     1501 ttacatagta aagcaagaac ttaaaggctt tgataaagga gtattatata aaccaatagt
     1561 taaacgttca atagtgtctt ggataattgg tatattaata acttaccttt caacatatgg
     1621 atttataact cttactacta tagcaccttt agatggtttt atagcagggt ttgtagtaca
     1681 atctattatt ggaaaagtgc tttgttcaac aaaaaataag caagaaatag acaaagcagt
     1741 ataaagaaat ttgtttataa agtataaaat aaattagaaa tatgaggaga aagtaacatg
     1801 agatttttaa cagaagaaag tgtagataaa atagcagttg gtgcagcagt gcttggaact
     1861 ggaggtggag gtgacccata tgtaggtaaa ttggttgcaa aacaagccat taaaaagtat
     1921 ggtcctgtaa aagtaataag tttagatgaa cttgatgatg atgctttggt agttccagta
     1981 tctggtatgg gttctcctgt aattacaata gaaaaattac tttcagaagt agagttaact
     2041 acccctttag aaataatgga gaaattatta aatagaaaag tggatgttat aattcctata
     2101 gaaataggag ggataaactc acttatgcct atagcagttg ctgctaaaaa aggacttcct
     2161 atcttagatg cagattctat gggtagagct ttccctgaag ctcaaatggt aactttttat
     2221 cttgaaggat atgaggcatc tcctgccgta atgtctgatg aaaaaggtaa ttctgccatc
     2281 ctttatcctg tggatggaat ttggtcagaa agattagcta gaactttaac tgtagaaatg
     2341 ggaggaagtt cttctattag tgactataat ttaagtggag ctcaagttaa aaaagctgct
     2401 ataggtaata ctcttacaat agctgaaact ataggagggt ttttacttga aaataaatct
     2461 gattcaaaag gtgctgttga gaatatacta aaagaattgt gtggatacaa gttatttgaa
     2521 ggaaaagtaa ttgatattaa gagagagttg aaaggtggat ttactagagg acatgccttt
     2581 tttgctggga ttaatgagta tgatggagag tattctatat tattccaaaa tgagaattta
     2641 attgccaaaa aaggtgatac accactttgt attacaccag atttaatagc agtattagat
     2701 ttagaaactg gatttccaat aactacagag agaataaaat atggctctcg tgttatggta
     2761 gtagcttttc cttgtaatga aaaatggaga acagaaaaag gaattgagac agttggacct
     2821 ggatactttg gatatgatgt ggaatataaa acagtagaag aattacaagg aaaataaaag
     2881 tttaggagga aattttgatg tatcgtgtag gaattgacgt tggaggaaca aatacagatg
     2941 cttgtatact ggatggggaa ttaaaagtaa ttcattcagt taaggtggca actacaaaag
     3001 atgttgaaac tggtgtttac aatgccttaa aaaaagttat agatgaaagt aagattgacc
     3061 attcattaat aaaatatgct atgcttggta caactcattg tacaaatgct attgtagaaa
     3121 gaaaaagact taataatgtg gctatgataa gaataggaaa gccggccact actgccattc
     3181 ttccatttat agattggcca gaagacttaa gagaaaaaat agaattagat tcaatattgg
     3241 tatctggtgg atatgagttt gatggcagaa aaataaatga actttgtaaa gatgaagttg
     3301 tagagttttg caatagaata aaaggcaaag tggaaagtgt tgcaataagt ggagtatttg
     3361 ctccagtaaa taaacaacaa gaagaagaag ttgcaaaatg ggttagagaa attttagggg
     3421 atattcctat atctttatca aatgaaatag gtaatatagg tatattagaa agagaaaatg
     3481 gagctatttt aaattctgca ttgtctcaag taggtaaagc tgtatcttta ggatttgaaa
     3541 aagcacttaa agatttaggt atagatgctc aaatatattt cagtcaaaat gatggaacat
     3601 taatgaattt agagtataca atgaaatacc caatttttac aattggatgt ggaataacaa
     3661 attctataag aggagcttcc ttcttatcaa aagtaaaaaa tgcaatagta ttagatgttg
     3721 gtggaactac aagcgacttg ggtgtactat ataatggatt ccctagagaa tcatctattc
     3781 cagttacaat tggaggagta catacaaact ttagaatgcc agatgtttta actattggtc
     3841 ttgctggtgg tacatgtgta aaaggcgaaa aagataatat aaagataggt cctgaaagtg
     3901 tgggatatag aataactgaa gatgcaataa tatttggtgg agacactcta actttatcag
     3961 atgttgttac aaaattagat atggcgtttg aagatagaaa aagcaatgtt gaaaacttag
     4021 ataaagagta ttgtaatgag gtatataaga aggttatttt aaaattagaa gatgctgtag
     4081 accaaatgaa gactagtcaa gatgatgttg aattggtttt aactggtgga ggaagtataa
     4141 ttgttcctga aaaactaaaa ggcataagca atgttataag acctcctcat tatactgctg
     4201 caaatgctat tggtgcagct ttaggacaaa taagtggaga tgtagaaaaa gtatacagtt
     4261 tagataaaat gtcgagagaa gatgccatag aagatgcaaa aaaagaggct gtaaataaag
     4321 ctctaaaagc tggtgctaaa gaagatacta tagaagtatt aaatatagaa gatgttccac
     4381 ttgcatacct tcctggtaat gcacttctta taaaagtaaa agttgttggt gatttaattt
     4441 aaagaataaa tataagtatt tccagaatta tataaatatt tctggactca tttttaaact
     4501 tattttatca ataaaaacag cataactttt gactatattc ttataagttt ccgaacagcc
     4561 gatatatacc tattttttga ggtttatcgg ctgtttgtta tcttttatcg cattccaact
     4621 atttttctta ccgtatttgc tgtacgaatt gtaatcttat cacttacatt tgaactggca
     4681 gtctttgacc accaatttgt ctttttataa tctttacgac taaaagacca aaagatagca
     4741 tttttataat gatatacttt ttcatactct tctttaggat ttccaacctc atcataaaac
     4801 tcgtcataag ataagtcagg aaacataaaa ataagattat catatatttc tttgttgtta
     4861 tctccccacc aattaggagc attatttagt atatctttta actcttcttg tgaaataata
     4921 aaaactggta tttccaattc aaactgtttt tttatcatta atcttattgt atttgagata
     4981 accttgctgt catctacatc acttaaaaag acaacattac cactattaag gtatgtagaa
     5041 acttctgaat aaccaagttc tacaaagctt gcttttaatt cagacataat tatcttgttc
     5101 ttaccactta tattgatacc tcttaataat gcgatataac ttttcatata ataatttcac
     5161 ctctaaacat tcacattttt ctatgtagta caaacttgaa tttgcatctt ttataattct
     5221 ttcaaaactt cttcatatgt aattttttta atataaaaaa atcggtgctt aatttgtacc
     5281 gatttttgtt tttcctagta aagaactcgt ttttttgctt ctgctatagc tctagcagca
     5341 ctttttgctt gcattctttt ttcttcttct tttttaaatt catttattgt ttcatttaga
     5401 ttcttatcat gttcattttt ttctttatct tcgttttctc tgtatactaa tatataatct
     5461 attattgatt cgtttattat ctgagatata cttttatttt cattaattcc tattctctta
     5521 acttccttaa gtagagtctc atcaatatga accgttgttt gctttgtatt ttttgccacc
     5581 ttaatcacct cttaaaaaaa attctaacat taaaattttt tttagtcaat aaattttaat
     5641 attaaaattc tttaaatgtt ttatttgatt ttctaaaatt ttctattgta tttatcatct
     5701 tattttttat attttcacct agtacagaag actttagtct aggattttcg atatggtcat
     5761 aaattggttc taaaaattca acttcgacag gaacagagtt aattttacca ataggttggt
     5821 atccttcata agtatctttt gaatttttta ttacgagtgg aacaataggg cattttgctt
     5881 tatatgctat ctttaaagct ccacttctaa actccgaaac caatgaattt ggttctttaa
     5941 tccatgttaa atctccttct ggaaacactg ccatactttg acctgtaagt atattttgag
     6001 aggcttgggc tatactcttt ataccctcac gattgttttt tctgtttaca tatacacatc
     6061 ttagtaattt tgcccattct ttaaatatag gtatatttct ccaaactggt tcatctgcaa
     6121 ttacacatcc aattggtctt tctacacttg ctgttagtat gaagctgtca agcatactag
     6181 aatgatttac aacaaataga agtggttctt ttggcagtgt ttccttacca attatattaa
     6241 gctctatatt cactatatct aaagattttt tagcttgttt ttgcataaat tcaaattttt
     6301 cctttaagct aaatttatca ggattctttt taattttaat aagcctaggt atagaagatg
     6361 taaaacctat aagttgatat gtagcaaatt ttaagtaatt attcataaaa ttatctcctt
     6421 aaaataaaat tatttattac tgtaaagatt ttaacataaa ataaaattta agtctatttt
     6481 aattcaattt tgtaacatat attttaaata gacagagatt gagattttat atttccaatt
     6541 ttagtaatca gattttatca ataaattact attctattaa tttaattaaa taacctttaa
     6601 atttaattat aaaaactgtt gcagaaattt ctacaacagt ttaaaaaatt taccttacaa
     6661 aaacttattt tataacaaga gagtatatta taaggctagt acagcccatt ttttgcactg
     6721 agaaattctt ttgccatcat caccttgttc tttgtcatat gcaaattgat taagcatttc
     6781 acatggaaat gtatcacata gaccacaatg tgtaaggttt ttatcttcac aacaagattt
     6841 tacaggacat tgttcacccc aaaatggttt ctcaatctct gtacaacctt tacaattgac
     6901 tttttccttg tatccacatt cataacataa aattccacat cttgattcta tcattttaag
     6961 tcactcctta aatataaatt ttatatgatt gtatatcaaa atttatactt ttcattgtaa
     7021 aaatcggaca aattttttat ataggttgtt ggattaacac cacaaataga tttaaaatca
     7081 tttataaaat gagattggtc gtaataacca atatcttgtg ctaaagttgc aaatggtatt
     7141 ttattatttt gtatttcttg taatacaagg tttatacgaa gtaatctaag gtatgattta
     7201 acactcatac caagagaatt attaaaaatt ctatttaaat gtcgttcact ataacaacta
     7261 atttgtgaaa tgtttttaac agacataata gagttttgtt ttctagcatt ttcaagaatt
     7321 ggtatagtca tatctactat atttgatttc aataagtatg ataagaataa catatctaat
     7381 tgttctacta attcctttat agtacttgtt ctttcaaaaa tggaattaac ctctaagcta
     7441 aataatgtgt taaaatcttc aagtgaaatt tttaaatctg tagattctct ttgagaaaga
     7501 cctgtcaaat aatatactcc acctgggcga aactcaacga atactctaaa aagtacattt
     7561 tctatatcat tttttacaat agtagttttt gttgtggctc cccagaatgc gctttctata
     7621 ccttttttgt caaatttaaa tatcatacat ccactagcat caggtattaa tgtaagattt
     7681 tctttaatac ttgtatctgg aatagatatt gtatagtgtg ctatatactg tcttaacaat
     7741 ttatgtggca aaatatatgt atactgtttg caggtattta aaatgttatt gattatttta
     7801 ttcataagta tatcctcc
//
LOCUS       DAYAOX010000169         7813 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.170, whole genome shotgun sequence.
ACCESSION   DAYAOX010000169 DAYAOX010000000
VERSION     DAYAOX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.170"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..333
                     /locus_tag="ACGI57_002842"
     CDS             <1..333
                     /locus_tag="ACGI57_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFG9717910.1"
                     /translation="RVVNVKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKP
                     LDEAIQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNL
                     KKHMDNRE"
     gene            341..1297
                     /locus_tag="ACGI57_002843"
     CDS             341..1297
                     /locus_tag="ACGI57_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HFG9717911.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            1308..3302
                     /locus_tag="ACGI57_002844"
     CDS             1308..3302
                     /locus_tag="ACGI57_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFG9717912.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            3303..3752
                     /gene="rplI"
                     /locus_tag="ACGI57_002845"
     CDS             3303..3752
                     /gene="rplI"
                     /locus_tag="ACGI57_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFG9717913.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            3771..5099
                     /gene="dnaB"
                     /locus_tag="ACGI57_002846"
     CDS             3771..5099
                     /gene="dnaB"
                     /locus_tag="ACGI57_002846"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFG9717914.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            5304..5612
                     /locus_tag="ACGI57_002847"
     CDS             5304..5612
                     /locus_tag="ACGI57_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717915.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            5694..6983
                     /locus_tag="ACGI57_002848"
     CDS             5694..6983
                     /locus_tag="ACGI57_002848"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFG9717916.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            7309..7384
                     /locus_tag="ACGI57_002849"
     tRNA            7309..7384
                     /locus_tag="ACGI57_002849"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7342..7344,aa:Lys,seq:ttt)
     gene            7391..7465
                     /locus_tag="ACGI57_002850"
     tRNA            7391..7465
                     /locus_tag="ACGI57_002850"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7424..7426,aa:Glu,seq:ttc)
     gene            7470..7545
                     /locus_tag="ACGI57_002851"
     tRNA            7470..7545
                     /locus_tag="ACGI57_002851"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7503..7505,aa:Val,seq:tac)
     gene            7579..7653
                     /locus_tag="ACGI57_002852"
     tRNA            7579..7653
                     /locus_tag="ACGI57_002852"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7611..7613,aa:Thr,seq:tgt)
BASE COUNT         3120 a          819 c         1451 g         2423 t
ORIGIN      
        1 agggttgtaa acgtgaaaat agataaaggt tctgagataa cgagaaacat aaagataata
       61 gaatggatga aaacagaaat attaatgagt gtaagtgatt tgtttaattt attgtttaaa
      121 ggggtaaaac cattagatga agcaattcaa gatactttag caaatataat aatgataaca
      181 tatctcttag caaagagatt aggaattagt tttagagatg tggattataa agtcaaggaa
      241 aaaataaaaa ttggtataga tgaggaccat agcgttgaaa gatggtatgg agatttatct
      301 aatttaaaaa aacatatgga taatagggag tgatattaaa ttgaataata aaattagact
      361 atctaaggca atgtctataa tagttttagc tataataatc gctttagtaa tagcctatat
      421 tccaatgcta ggaatattta gtatgttagc agctgttcca tatgtaatta taggtgctat
      481 tactgataga aagtattctc ttatatctat tttggttaca ttttgtgtac tgattctatt
      541 tgcagacatt tcttatgcat taaatatatg tataatgtat tctttaccag gtatagtaat
      601 aggaaagatg ctaaagagaa gctttgaaca agaggacagt aataaattcg aaccgatata
      661 tggtggaacc ataatatttg tactttcaat gcttgtatat ttttttgtat taaagacatt
      721 tttaaatgtg aatctattag atgaagtttc taagatgata tcagaaattg taaatattca
      781 aaaaaatagt ttttctgcaa ctgaacttaa agtgtttgat aaaatgaagc cagatgagat
      841 tgtaagttat tttacaaata tgctaccaat gatgttattt ttacaagggt tgttatcagc
      901 gtttgtcaca tattatttga gtgtattttt tataaaaagg attacaaaga tgaatataag
      961 atttcctaag tttgctgatt tttatttacc gggaaatgct atactcacaa ctttcttact
     1021 ttatttatta gtcttattta ttcaaattat tggttcaaaa ttatatacag aattaataat
     1081 gactaattta cagctagttt ttaatttaat gtttgtaatt caaggtatag cagtttgcat
     1141 ttatttctta aagaaatgga taagacaggg accaaataaa attatgtttt tttcagggat
     1201 aattttatgt ttatttggat ttatgggaat atcttttgtt ggtatggtag atagtataat
     1261 tgactttaga aaggtaagaa gctataaatc cacttaggag gatagaaatg agtaataaac
     1321 aaacctttaa attaaatatg ccagaaataa atttatatat aattgttatt ggtatttcta
     1381 gtataatttt gctttattac aacttgtatg tagggtgttt atttttctgt atttttgtat
     1441 atatggtttt tcacaattgg agaactacta atattagacg tcatgaatgg acagagtata
     1501 ttcaaaattt atctttagat atagatgaaa caactaaaaa agccataata aatcttccaa
     1561 taccattatg tattttagaa tttgatggaa atatatcctg gtataatgga aagttttatg
     1621 atatgattgg tcaaaaagat ttacttgata aaaatataga ggatatagtt aaaaatctta
     1681 atttaagaaa agtattaaat gaaaataaag agatgtacac agaaataaac tataaagaaa
     1741 aagaatatac aataatttat aatgttataa aaaatgatca agaaaaaaat cctaaatatt
     1801 tgatgatatt atactggata gacaagactg aatatttaaa agtaaaacaa aattatgatg
     1861 atgaaaaaaa tgcaatgatg ctaatacaag tagatggata tgatgaagta ttaaaaagtg
     1921 cagcagaaga taagagagct ttaattaatg ttgaagtaga gaaaatactt tctgctttag
     1981 aattaaattc aaatggtgca ttgagaagaa cttcaaaaga caaattcttc ttggtaatgc
     2041 ataaaaaaga gttaaaaaaa ttggaagctg agaagttttc tattttagat acaataagac
     2101 atattgatta tggaaataat cttcctgtta ctataagtat aggaattggt atagatggag
     2161 atacattaaa tgagaaccta aaacttgcta cgggtgctct cgatttagca cttggtagag
     2221 gtggtgacca ggctgttgta aaaacaaaag ataaatttgt gttttatgga ggaaaatcta
     2281 aagctgtaga aaagaaaacc aaagtaaaat ccagattaat aggtcatgct ttaagagagg
     2341 ttatacaaca aagtgaccaa gtttatataa tgggacataa atatcctgat atggatgcta
     2401 tgggagcagc agttggtgtt tatgatatat gtaaatcctg taacaaaact gcaaacatag
     2461 ttttacaatc tgtaaatgaa tctatagaaa tatttataaa taaaataaat gaaaataatt
     2521 actataaaaa gttgtttatt ggaaaagagg aagctattga caactgcact aaaaatacat
     2581 tagttgtagt agttgatact catagaccaa attatactga atgtgaagaa ctgttaaagc
     2641 tatcagagaa ggtagtagtt atagaccatc atagaagagg tgtagagttt ataaatgatg
     2701 cagtgctttt attccatgag atatatgtat cctcaacgtg tgaaatggtt acagagttag
     2761 ttcagtatat ggatgaagat gtaacaataa ataaacttac agctgaaggg cttttagcag
     2821 gtataagtct agatactaaa aactttgcat tcaaaacagg tgttagaact tttgaagctg
     2881 cttcttacct tagaaaagtt ggagcagata ctatagaggt aaagaaattc ttcaattctg
     2941 atgtaaaaga tttcataata aaagctgaaa taattcaaag tactaaaata attaataata
     3001 gaatatgttt agcatattca agtactgaga tagatagtat aaatgttata atagctcaaa
     3061 ctgctgatga gttattaaat atcaaagaag tagaagcttc ctttgtatta ggtgaaaaag
     3121 atgatactat atttataagt gcgaggtctt taggacaaat aaatgtacat gtacttatgg
     3181 aaaaattagg aggcggggga cacatagata tagctggagc acagttaaaa aatgtttctc
     3241 taaaagaagc ttataagatg gtaaataaaa taatagagga gtatttggag gaggaagaat
     3301 agatgaaagt tatattatta aaagacgtaa aaggaactgg taaaaaggga gaaatgaaag
     3361 aagtaagtga tggatatgca agaaatttct tatttcctaa aaagatggct gtgcaagcag
     3421 atagtgtagc aataaaagaa ttaaatgaaa agaataaatc taaagaaata aaagcacaaa
     3481 aagagtatga agaagcagtt ttattaggta agcaaatgga agaaattaat atagaaatat
     3541 attcaaaagc aggagaaggt ggaagattat ttggttcaat aacttctaaa gaaattgctg
     3601 agcaattaaa gaaacaaaaa gacatagatg tagataaaag aaaaatatta ttagatgaac
     3661 caataagatc tttaggttca actttcgtag aaataaaaat acatcaaaaa gtaactacta
     3721 aaataagagt ggatgtaaaa gaaaaacagt aataactaga ggtgattaag atggaagata
     3781 tgacgagaat tcctcctcat agtgtagaat cagaacaatc aatattgggt tctatacttc
     3841 tagataaaga tgctataata acggttacag aaactataaa gcctgatgat ttctataaag
     3901 aggctcacaa aataatatat gaatgtatga taactttaag taataaaggt gagcctatag
     3961 acttgataac attaacagaa gaactaagaa aacaagggca tcttaatgat attggaggaa
     4021 tcagttatat tacaagtctt tctactatag taccgacgac ttcaaatgta aaatactatg
     4081 ctgatatagt aaaagaaaag tctgtactta gaaagcttat aaaagcttct aatgaaataa
     4141 taaatctagg atatagtgga gcaactaaga ttgaagatgt tttagaacaa gctgaaaaga
     4201 gtatatttga tatatcacag gagaaaacta gtgatgattt taaatctata aacttagttt
     4261 tgatggatgc ctatgatatg atagaaaaat tatacactaa caagagtgat gtcactggta
     4321 taactactgg ttttaaagat ttaaacaaaa aaataaatgg tcttcaaagg actgatttaa
     4381 tattaattgc agctagacca gcaatgggta aaactgcctt ttctttaaac ttggttcaaa
     4441 atgcagcctt aaaaggtgat gcctctgtag ctgtatttag tttagagatg tctaaggaac
     4501 aattagtaca acgtatgtta tcttcacaat ctagtgtaga attaaaaaaa ataaagactg
     4561 gaacacttaa tgataatgat tggcctagaa ttatagatgc tatggcagta ttatcagatg
     4621 ccaaaataca catagatgat actcctggta taaaaatatc agaactaagg tcaaagtgta
     4681 gaaagttaaa aatagaaaaa ggactagatt tagtgcttat tgattatctt caacttatgg
     4741 aaggtgaagg taataatgaa agcagacaac aagagatatc taagatatca aggtcactaa
     4801 aaatattagc aaaagaatta aactgtcctg tagtagcact atctcagtta tcacgtgcac
     4861 cagaacaaag agcagaccat agacctatgt tatctgattt aagagagtct ggagcaatag
     4921 aacaggatgc agatatagtt atgttcttat acagggacga atattatcat gctgattctg
     4981 agagtaaaaa tataggagaa gtaataatag caaaaaatag acacggtgaa actggttctg
     5041 tggaacttgt ttggcttggt gaagttcaaa gatttgggga taagttgaga gacctataat
     5101 ctaacgaata tccacagaac gtcctttctt tctacaggaa aaatgtggat acatgtcact
     5161 aaatatgtcc aaacattgaa atttagctaa aaaactatcc acactatcca cagatggcct
     5221 gttgataagt gtgaataagt agataaagca agcttttggc ttgctttatc tgtgtataaa
     5281 tattaattat taggaggaat aatatggaaa aaataagctt aaaatatata tatcccaata
     5341 taattaaagt acttgatgaa ataaacttat ttagagtaat tgacaataat ttaagagaat
     5401 ctattgttgt ttatgctaat aatgtggata atcagtatca tataaacatg actaatacaa
     5461 actttggaaa tataattaac atatgtaaat tagaaaaact tttagatgtg gataaattta
     5521 tggaaaaagt tataaaatat gaaaaagaaa taatagagaa agaagagttt tcaaaaatag
     5581 aagaatatat gttaaatatt ggagaatact aaaaaagttg aaaaaaatac gaatgtttga
     5641 tagaataagt aagtaaagtg ttcgagagta tataaaatat agaggtgaat tacatgaaaa
     5701 cagttgcaat tgttggttct caatggggag atgaaggtaa aggtaaagtt atagattatc
     5761 ttgctactca agcagatgta gtagtaagag ggcaaggtgg aaataatgca ggacatacat
     5821 tagttgtaga aggtaaaaaa tacgcattac atctaattcc atctggagtt ttaaatccaa
     5881 atacagtgaa tataatcgga aatggaatag tgtttgaccc taaaggattt ttagaagaat
     5941 tggaaatgtt taaaacggat aatataagta cagaaaatat aaaaataagt gatagagcac
     6001 atgtcatatt cccatatcat aaagaactag atgcattgtc agaagaagcc agaggcgatt
     6061 taaagatagg tacaacaaaa aaaggtattg gaccttgtta tatggataaa acagagagat
     6121 ctggtattag aatatgtgac ttaatggata aagataaatt tgctataaaa ttaaaagctc
     6181 aaatagatgc taaaaatgaa atagttaaaa atatatatgg aaaagaagaa ttatttgatt
     6241 ttgaaactat atataacgaa tatctaggat atgcagagca aataagaaaa tatgtggcag
     6301 atacgtctgt tattgtatat gatgcagtaa gagcaggtaa aaaagtattg tttgaaggag
     6361 cacaagggac tttattagat ttagatttag gaacttatcc atttgttaca tcttctcatc
     6421 caacatctgg tggatttgca attggtgcag gtatagggcc aaatatgata aaagacgttg
     6481 ttggtatagt aaaagcatat actacaagag ttggagaagg accgtttgtt acagagcaaa
     6541 taaatgaaac tggagataag ataagagaac aaggacatga atttggggta actactggta
     6601 gaccaagaag atgtgggtgg tttgatgcag ttatagttaa gtatgcagct agagttaatg
     6661 gattgactag tatttcattt atgttacttg atgtattaac tggatttgat aagataaaag
     6721 tttgtacgtc ttataaaatg ggcgataaga taataacgga tttcccagct tcattagatg
     6781 atttagcaaa atgtgaacct gtatatgaag aattagatgg atggaatgaa gatataactc
     6841 aaatagataa ctttgatgac cttccagaaa atgcaaagaa atatgtagca aaaatagaag
     6901 aactagttgg agtaagtgta gatatggttt ctgttggtcc aaatagagct caaactataa
     6961 taagaagaaa tatatttgct taatatttta tcaattaaat aatttaaaat agttgaagaa
     7021 gagtatctca agataatgtt tatagttatt agagatgctc ttttatattt gacttacatt
     7081 gattttttat taagttaaag ctacttatac atattctaac tgaaattaaa gttgaattta
     7141 tgtagataca tataggtttt ttaaaaagaa ttttgttgaa aataaatggc taaagatgat
     7201 tttaattata ttctaaaaaa agtaaaaaaa actattgact aaaaattata tagacgctat
     7261 aatataactt gtgagtgaaa gaaacgacga aaaaagtcga aaaaatgtgg tctattagct
     7321 cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc
     7381 accaaaatat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg cggtaacagg
     7441 ggttcaattc ccctacgagt caccagtatg ggactatagc tcagctggga gagcacctgc
     7501 cttacaagca gggggtcaca ggttcgagcc ctgttagtcc caccaaattt tgaattagag
     7561 ataaatacta gattatatgc tggtgtggct caacggtaga gcagctgact tgtaatcagc
     7621 aggttgtagg ttcgattcct atcaccagct ccacaaagta cataaattca aattaatata
     7681 attttctatg ttacaggata cttttaagta tcttttttta atttcatttt tattgtatta
     7741 aaaatgaaat taaacataca acaaaatttt gaattacttg caatgtgata gttttaaata
     7801 taaaatttag aat
//
LOCUS       DAYAOX010000170         7772 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.171, whole genome shotgun sequence.
ACCESSION   DAYAOX010000170 DAYAOX010000000
VERSION     DAYAOX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.171"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            234..431
                     /locus_tag="ACGI57_002853"
     CDS             234..431
                     /locus_tag="ACGI57_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9717917.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            460..1536
                     /gene="vorB"
                     /locus_tag="ACGI57_002854"
     CDS             460..1536
                     /gene="vorB"
                     /locus_tag="ACGI57_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFG9717918.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            1539..2258
                     /locus_tag="ACGI57_002855"
     CDS             1539..2258
                     /locus_tag="ACGI57_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFG9717919.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            2271..2810
                     /locus_tag="ACGI57_002856"
     CDS             2271..2810
                     /locus_tag="ACGI57_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFG9717920.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            2967..4025
                     /gene="buk"
                     /locus_tag="ACGI57_002857"
     CDS             2967..4025
                     /gene="buk"
                     /locus_tag="ACGI57_002857"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFG9717921.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            4043..4936
                     /locus_tag="ACGI57_002858"
     CDS             4043..4936
                     /locus_tag="ACGI57_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HFG9717922.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            4966..6174
                     /locus_tag="ACGI57_002859"
     CDS             4966..6174
                     /locus_tag="ACGI57_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9717923.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            6198..7457
                     /locus_tag="ACGI57_002860"
     CDS             6198..7457
                     /locus_tag="ACGI57_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9717924.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT         3004 a          878 c         1562 g         2328 t
ORIGIN      
        1 aatatcagtt taaatgtagc ctatttttct ttaatctaaa attaaatatt cttaaaattt
       61 aaaatgttat tgaaatatat aagcgaatgt gttatctttt aattaagcaa taaaaataga
      121 acaaggttct atactataga acaaaattga tgcatatata gtatttatac aaaatataaa
      181 tattatatat ttaaaattaa gtaaaaatat tacataaagg ggagatttga gaaatggaaa
      241 aagtcaagtt gagtgttgaa agatgtaagg gatgctatct ttgtattgaa gcttgtaaaa
      301 aagatgcaat aagtctttct gactacacaa ataaaaaagg ctttgtaact attaaagtcg
      361 atgaagaaaa atgtattcaa tgtggcgctt gttatacaat gtgccctgat ttagtatttg
      421 agatattata aattattttt attagagggg agaaaaggta tgcaaagttt attaattaaa
      481 ggaaatgaag ctttagcaga aggtgctata cgtggtggct gcagatttta tgcaggttat
      541 ccaataacgc cacaatcaga aatattagaa atgatggcat ggagacaaaa agaagttgga
      601 ggagtattta tccaaggtga atcagaaata gccagtgtaa atatggttat gggagcaagt
      661 gcacttggag caagagctat gacttcttca tctggtccag gattttcatt aaaacaagaa
      721 ggtattgcat attgggtatc agctggtttg ccagcagttg tagcatgtgt gcaaagatat
      781 ggtataggtg atggatttat aggagctggt caagataatt attggcaagc agtaaaaggc
      841 ggtggaaatg gagattatca tttaatcgtg ttagcaccta atagtgttca agaaaatatg
      901 gatatggcat atgaatcttt tgaattggct gaaaaatata tgcatccagt acttatactt
      961 tctgatgcta caataggtca aatggtagaa ccttgtatag caccaccaat gaaagaatac
     1021 gatatggata aagctccatg ggctgcaaaa ggaacacctg taggagaaaa aaataaagta
     1081 ataactgata taacatattt tgaagaccaa gatacatggc aaaaagggta tatagaaaaa
     1141 atgagaaaag ttcaagaaga tgaacaaaga tgggaagaga tagaaataga agatgctgaa
     1201 gtggtatttg tggcatacgg tatttcatca agaatagcag aaggtgcagt tagagatgct
     1261 agagcaaaag gaatgaaatt aggtctaata agaccaaaaa cattgtggcc ttacccaaga
     1321 aaagcattta agaaattaag tgattcattg aagggacttg taacagttga aatgaacatg
     1381 atggcacaga tgaaggaaga tgtaattata gcatgtgata atagatttcc agtatattca
     1441 gtagcaacag ctcaatatat ggcagaagta aatcaattaa tagattttgc tcaacagatt
     1501 atagatggaa aagctaagca agaggaggta ttttaattat ggctaaaaaa gcaccagcaa
     1561 taatgatgtg tgaaaatatg ttctgtggag gctgtggaca ctctatagtc gatagaatta
     1621 ttggagaagt attaacagaa atggatatat gtcaagatac aattatatgt tcagatatag
     1681 gttgtaatca tatgtttcaa tttagtatga atgtagatgt tgtcgttcca cctcatggta
     1741 aaatgggagc agcaatggca gctatgaaaa gagttagacc agataaatat atatttacaa
     1801 tagctggaga tggtggagct tatgcaatag ggcttggaga aactatgtca gctgctacta
     1861 gaaatgataa tgtaatattt cttgttatga acaataatat gtttgcaatg acaggagggc
     1921 aaatggctcc aactactata gaaggtcaat atacagcttc tactccagcg ggtagaaatt
     1981 acgcagatca tggttcaaca tttgatgttg taaaagttat gggaaattta gatatagcat
     2041 atctagcaag aggaacaata actaacccta aagaaataaa tacaacaaaa agatacatca
     2101 aaaaagctct agaaaaacaa aaagaaaaga aaggtttttg cttaatagaa gtgcttgtac
     2161 catgtccaac aaactgggga ttaaagccag tagatgctat gaagagaatt gaaaatgaac
     2221 ttgttccaaa gtatccactt ggagaattta ttgattaggg aggaaataat atgttgaatg
     2281 aaattatatg tgctggtttt ggaggacagg gagttcttac agcaggaaaa attataatgt
     2341 atgttgcata taaggcagaa aagaaaatta catggttccc atcttatgga aatgagatga
     2401 ggggaggaag tgcaaattgc aatgttgttg taagtgatga aaaaattgca aatccttgtg
     2461 taaaaaatcc agatatacta ttgggaatga acagcgtatc agtagataag tatgaaagtt
     2521 taatgaaaaa aaatggttat atgtttgtca atgaatcact agtaagtaag gatagaaagt
     2581 acagagaaga cattaatgtt ataaaagtac ctgttactga aatagcacaa aatttaggca
     2641 atgaaaaagc tgctaacatt tgcatgttag gtgctatggt aaagagtttg gatttattta
     2701 caaaagaaga atttgaaagt ggtatgtgtg aatactttga aaatcaaggc aaaggtaaat
     2761 tcaattctaa aaatgtagaa gcatttgaag ctggatacga ctacgaatga atattttaaa
     2821 tatttaaaat aattattgaa atacttattt ttatatgtta gtatttaatt aataaaatat
     2881 agaacgtcgt tctggacaat gggacgaagt ctttaggtcg gaatacaaga acggcgttct
     2941 gtataataga actagggggt gttaaattga aagttttagt aataaatcct ggtggtacat
     3001 ccacaaaaat ttctgttttc caagatgaaa atgaaatttt gaaaaaaaat ataacccata
     3061 caagagaaga tttaaaaaat ttttctaaag tatttgatga atatgattat agaaaacaac
     3121 tgatagtaga tattttaagt agtgagaatc atagtataaa ttcttttgac gcagtagttg
     3181 gtcgtggtgg acttatgaag gctataaaag gtggtaccta tactgtgagt gaagaaatga
     3241 ttgaggatat gagaaatgaa ataaatgggg aacatgcatc aaatttaggt gcattattag
     3301 ctaaaactat agctgatgaa ataggagtac aatcatttgt agtagaccca gtttctgtag
     3361 atgaatttga tgatgtatcg aggattacag gaatatcaga catagaaaaa gcaagttggt
     3421 tacatgcatt aaaccataaa gctgtttcaa gaaaaattgc acaaaaacta gggggaaaat
     3481 acgaggatta taactttatt gtagctcatt taggttcagg aatatcgatt gtagcacata
     3541 aacaaggcaa aatgattgat ggaagtggtg gtaggtctga tggtccattt tctccagaaa
     3601 ggagtggtgg attacttgca tatcctctta tagaattgtg ttactccgga aaatatactc
     3661 atgatgaaat ggtcgcaaaa gtaagttcga ttgggggaat gtacgattat ttaggaacaa
     3721 aggacatgat agaaattgaa aacagaatca aggagggtga tgaaaaggca aaacttataa
     3781 tggatgcatt tatttatcaa gtagcaaaag aaatttctat gtatggagct tcactttcag
     3841 ggaatgtaga tagagttatc ttgacaggag ggatatctca ctcagaaatg gtagtcaaag
     3901 gagtagcaga aaaagtttca tatctagctc caatagagat agtaccagga gagatggaaa
     3961 tggaggcact tgctctaggg gcacttagag tacttaatgg agaagaaata gcaaaaactt
     4021 attaatattt aggagaaaga gtatgttaaa agattttaat aatttattag aagaggctaa
     4081 aaagcttgaa cctgttattg taagtgttgc tgttgcagaa gacaaagaag tattaaaggc
     4141 tgtgaaatca gcaacagatt taaacattat aaagccaatt cttgtaggag acaaggataa
     4201 aatagaaagt atatcaagag aaattggatt aaaaggtcat gaaataatag gttgtcaaaa
     4261 tcccgatgaa tcagttaaaa aagctgtaga gatagttaaa aataaggaag ctaaagttct
     4321 tatgaaggga ttagtaaata caagtgtcta tatgagagct atattaaata aagaaacggg
     4381 tcttagaaca ggaagattat taagtttgct tgcagtatat gaattacctc aatatcataa
     4441 acttctttat tgtactgata gtggaataaa tgtatcgcca aacttaaatc aaaaaaaaga
     4501 tataatgaca aatgcacttc ttactatgaa aagtataggc atggaaaatc ctaaaactgc
     4561 gatacttact gcaaatgaaa tggtagaccc taagatacaa tctacagtgg atgccaacct
     4621 acttgtagag atggaaaaat cgggagaaat accaaagtgt atagtggaag gtccaatagc
     4681 atttgatgta gcatttgatg cacatgcagc agaacataaa ggtattaaaa gtaatattgc
     4741 tggtgatgtt gatttattag tatttccaaa tatagaagca gggaatatat taggaaagtc
     4801 atggcttaga tttaataaag ctaagtgggc aggtatagtt ttaggagcaa caaatccgat
     4861 aattttaggt tcacgttctg acacagctga aataaaaata aattcaatag cattagcgtg
     4921 cctagcatca caataagaaa ttattattaa ggaggaaaaa gtattatgaa atatgcaaaa
     4981 agaatggata tggtaaaagc ttcagctata agagattctc aaaagaaaat agctcagaag
     5041 gtagcaagtg gtggaactgt tatatctttt gctgcagggc ttcctgaccc aaatttattt
     5101 ccaatagaag agataagtga aataactcaa aaagtactta atgaaagggg aaaatttgcc
     5161 ctagcatatg gacctactaa aggtgaagat gagttgttag atctattagt aaaaagaatg
     5221 aaagaagaag aaaatattga tacaaaagca gaaaatatta taataactac tgggtcacaa
     5281 caagggatag cattaagtgc tatgatttta ttagaaaagg gagatgtagt agttacagaa
     5341 aatccaagtt acttaggtgc aataaatgca tttagacctt atgaatgtga ttttttagga
     5401 gtagatacag atgaagaagg aatggtaaca gatgatttag acaaattatt atctgaaaat
     5461 tctaacataa aggtgatata tgtaatacca acatttcaaa accctacagg aaaaacatgg
     5521 tctttacaaa gaagaaaaga ttttatggaa gtcgtgaata aatatgatgt catagtaata
     5581 gaggataatc cttatggaga aataagattt acaccagaac ctctacctac acttaaatct
     5641 cttgatacta aagataaagt attgtacctt gggtcatttt caaaggtatt atgtccagga
     5701 tttagagttg cttggatgtg tggtaatgta gacatcatag ataaggcaga acttttaaaa
     5761 caaggtatag atttacagtc aaatcaattt tcgcaagtac aagtaataga atttttaaag
     5821 aaatacaact taaatgaaca tatagaaaaa ataagagcag agtataagaa aagatgtctg
     5881 ctaatgttag atgctatgaa gaaacatttc ccagaggaag ctaaatatac agaaccagat
     5941 ggaggaatgt ttatatgggt tgaacttcct gaaagtataa atgtagatga tttattagat
     6001 aaagccattg atgctggagt tgcttatgtt tctggagagt ccttctttgc aaataatggt
     6061 ccaaaaaata ctatgagact taactataca acaatgtcag aagaacaaat cgttaaaggt
     6121 gttgagatac ttgcagaagt tataaaaaaa gaactcaatt atgcttgtga ataaatataa
     6181 aaagtgaggg tttaaatatg ggagaaaaag aaaacggtat aaagtttgga aagttaatgt
     6241 ttcaacttgt tattatagca atatcttggg aattagttta tattatacca tttatccaat
     6301 atacattata tgatccaatc ctaaaagcat tagaatgtac aaacactcaa ctaggttttc
     6361 tacttacaat ttatgggtta ggtaatatat ttggagctcc tataggaggc tggttagcag
     6421 atagatttga ttatagaaag ataattttag gttctgtatt tcttaatgga gtagtatcat
     6481 ttttatttgc atttaatatg aactatccat ttgcagttgc tacttgggta ggttgtgcaa
     6541 tcacatcatt agttatgaac tatccttcta tggtaaaaat acttagagtt ataggaaaag
     6601 ataatcaagg taaggtttat ggatttaatg aagctatggt tggagtttct ggagtagtaa
     6661 tgggagcaat attcttatat atttatacat gctttgcaac tcctacattg ggtatgagat
     6721 gggtaatgat atctttagga atattatcaa tagcaatgtg tccagtatta tggtttgtaa
     6781 ttaaagatgt agatattaaa gaagaaagag aagaagcagg tgaaaaaatg tctgctggtg
     6841 attttataac agtactaaaa tcaccaaata cttggctagt aggaataagt atattctgtg
     6901 tatattcatt cacagttaca atgtcttatt ttacaccata tataacatct gtacttggag
     6961 gttctgtagc attatctggt gcattagcaa taataagaca gcatggtcta aaattatttg
     7021 gagcaccatt tggaggatat tgtgcagaca aattaaaatc tcctacaaaa gtacttttac
     7081 caatatatgt atttggtata gcagtaatag ttttattcct agtattacct gcatcaactc
     7141 caatgactat attcatagct ttaacatttg tagtaggtat attaggttac atgggaaaag
     7201 gtatatatta tgcagtacaa gatgaagtaa aagttccagt taaatattca gcaactacta
     7261 taggtatagc tgcagcttta ggattttcac cagatgtatt ccaatttgca ttaataggac
     7321 actggataga tacttatggc aataaaggtt acacatatac atttatattc caaatagcaa
     7381 ttctagttat tggaatacta tcttgtttat atatattaaa ggttaaaaag agaagagcaa
     7441 gtaaattaga agcttaaata ttcaatttgg taattaagta tatataaata caatacttat
     7501 tttgatgaat gtgattgtat aaataacata aaaaatatca atagattaag ttttataata
     7561 atgtttagag aataaagtat aaaaatcaaa atgttatcaa gttttctagg ttttaacata
     7621 aagttacccc tgaaggtgta aaattgagtg gcaattatta tctattttct tacctacata
     7681 aacctattta atttaaattc taagagattt tcaaaataga tggtagttgt actcaatttt
     7741 aaacctcata ataaatacga aaatttaaaa tc
//
LOCUS       DAYAOX010000171         7718 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.172, whole genome shotgun sequence.
ACCESSION   DAYAOX010000171 DAYAOX010000000
VERSION     DAYAOX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.172"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            15..326
                     /locus_tag="ACGI57_002861"
     CDS             15..326
                     /locus_tag="ACGI57_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFG9717925.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      419..511
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            611..1405
                     /gene="thiD"
                     /locus_tag="ACGI57_002862"
     CDS             611..1405
                     /gene="thiD"
                     /locus_tag="ACGI57_002862"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFG9717926.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1429..2217
                     /gene="thiM"
                     /locus_tag="ACGI57_002863"
     CDS             1429..2217
                     /gene="thiM"
                     /locus_tag="ACGI57_002863"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFG9717927.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDALSKNSNIEFLN"
     gene            2240..2872
                     /gene="thiE"
                     /locus_tag="ACGI57_002864"
     CDS             2240..2872
                     /gene="thiE"
                     /locus_tag="ACGI57_002864"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFG9717928.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3011..3313
                     /locus_tag="ACGI57_002865"
     CDS             3011..3313
                     /locus_tag="ACGI57_002865"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9717929.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3379..4128)
                     /locus_tag="ACGI57_002866"
     CDS             complement(3379..4128)
                     /locus_tag="ACGI57_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717930.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDIMPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4122..5084)
                     /locus_tag="ACGI57_002867"
     CDS             complement(4122..5084)
                     /locus_tag="ACGI57_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9717931.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5088..5546)
                     /locus_tag="ACGI57_002868"
     CDS             complement(5088..5546)
                     /locus_tag="ACGI57_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9717932.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5902..6258
                     /locus_tag="ACGI57_002869"
     CDS             5902..6258
                     /locus_tag="ACGI57_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9717933.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6330..7046
                     /locus_tag="ACGI57_002870"
     CDS             6330..7046
                     /locus_tag="ACGI57_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717934.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7039..>7718
                     /locus_tag="ACGI57_002871"
     CDS             7039..>7718
                     /locus_tag="ACGI57_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9717935.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYI"
BASE COUNT         2998 a          900 c         1262 g         2558 t
ORIGIN      
        1 ggatgtgatt taaggtgtat tttacatata ttataagatg taaagatgat tctctataca
       61 ctggatatac cagtaatata gttagaagaa tgaatgaaca taaattagga attaattcaa
      121 aatacacaag agcaaaggga tttgaaaaac tagaagttta ttttgtgacc aacacaaaaa
      181 gcaatgctat gaagttagaa tactatataa aaaaattaac tagaaataaa aaattgtcta
      241 taataaaaaa cccaagtata cttataaatt taattgataa taaagaagat tatattattg
      301 gtaaggaaat agaacaatta acataaatat ttatagtaac attataatgg gtgcatatgg
      361 tgtaaaaact tgacaataaa tactattgta atataatgaa gttataaata tagtaaatta
      421 aaatagctag gggagccaag agctgagagg tataccgacc cttacacctg atctggataa
      481 taccagcgta ggaaagctta gtaaaaattc aagtatgtat tatttgtgat atatgctatt
      541 tctaagctat acctattgta ggtatagctt ttttaattaa gttatttaaa ttaaacttta
      601 ggaggcaaca atgagtaatt ataaaatacc aacacttaca atagccggct ctgattcatc
      661 tggaggagct gggattcagg ctgatttgaa aacgtttagt gcaattggta cttatggtat
      721 gagtgtaata acagctataa cagcacagaa tactaaagga gtttttgctg tagaggattt
      781 aaataagaaa ataataaaaa aacaaataga ggcagtcttt gaagatatac ctccaagagc
      841 tgtaaaaata ggaatggttt caagtcctga gattatactt gaaatagttg agaatctaaa
      901 gaaatacaat ccaaaatatt tggttgtgga cccagtaatg atatctaaaa gtggatatta
      961 tctattaaag ccagaggcaa aagaaaactt aattaagtat ttgataccac ttgcatacat
     1021 aattacacca aatataccag aggcagaaga aataacagga ataaaaatac ataatgtaga
     1081 tgatatgaag agagtaggag aagaaatact acaacttgga cctaaatttg tacttatgaa
     1141 aggaggtcat ttagatggag aagcagtgga tattttagtt ggtaaaaata tatttaaagt
     1201 ttataaaagt gaaagaattg ataaaaaaaa tacacatgga acagggtgca cattatcttc
     1261 tgcaataaca tcttatctag cattaggata tgaaataaca gaagcagtaa atctaagtaa
     1321 aatctatata acagaagcaa taaaaagaag ttttgatata ggtcatggag taggaccagt
     1381 acatcatttt tataaatttg agtaataaaa aaatattgga ggtagaatat gtataattta
     1441 attaaagatg taaaaaaatt aaatccattg gtaattcatt atactaataa tgtaactata
     1501 aatgattgtg ctaatgtaac tttagctgta ggagcaagtc ctcttatgag tttttcatat
     1561 gaggaagtag aagaaatggt aagtgtagca aactctgtag taataaatat aggaactatg
     1621 aactcaaata tgttggattt attcctatta gctggaaaag cagcaaataa gtacaataaa
     1681 cctgttgttt tggacccagt tggagtattt gcaagcaaag cgagagcaga acttacaagt
     1741 agacttttaa atgaagtcaa atttagtgtt gttaaaggga atgtatcaga aataaagttt
     1801 ataggtggct ttaatgtaag gggtaaaggt gtagattcat ttgatgaaga agaagattct
     1861 actgagataa taagaaagat tgctgaaaag cttgaatgtg tagttgtagc aactggaaaa
     1921 atagatataa taactaatgg taaaggtaca tataaaataa acaatggaac tgataaatta
     1981 aaaggaatta caggaactgg atgtatgact gctagcttga ttgcaagttt tatggctgtt
     2041 actgagaata tattagaagc agcaacaatg ggtgttctta caatgtcttt aagtggggaa
     2101 ttagctaatt taaataatcc accaataggt acatttaaag aaaatcttat gaatgctatt
     2161 tatcaaatgg atatagatgc tttaagtaaa aattctaata ttgaattttt aaattaataa
     2221 agagtaaggg agacttaaaa tgattgataa agaatcttta aaaaaatgtt taaagttgta
     2281 tttagtaact gactctgaaa tgcttaaagg tagagacttt tataaatgtc ttgaagatgc
     2341 aatatcttct ggaataacaa ctgtacaact tagagaaaaa aatgcttctg gaagagagtt
     2401 tttaagaaaa gctatgaaac ttagagaaat aactaaaaga tatggtgtta agtttataat
     2461 aaatgataga gtggatatag cacttatttg tgatgcagat ggagtacatg taggacagag
     2521 tgacattgat gttagagagg ttagaaagct tataggaaac aataaaattc taggagtatc
     2581 agcaagaact ttagaagaag ctatttgtgc aaaaaatgat ggcgcagact atttaggtgt
     2641 agggtctata ttcacaacat ctactaaatt agatgcaaaa tcagcttcgt ttgaaactgt
     2701 taaagaaatt aaagaaaaag tagacatgcc atttgtacta attggaggta taaatttaga
     2761 caatatagat aaacttaaat gtttagaaag tgacgggtat gctataatat cagctatatt
     2821 aaaagctgaa gatatctcta aagaagtgga aaagtggaca ttaaaaatat aaaattggct
     2881 ctggtagtga aaaaaatgga aaaatttgat ataatctaag agtaaaattt aagcaattat
     2941 aaatgataaa cttttctaca tatacttttt aatcaagata tatgtagatt tggttaaaaa
     3001 ggagctataa atgattagga tattgttagt gtgtgttgga ggcatgtctt ctaccctatt
     3061 ggtaaataaa atggagaagg atgcaaaaaa aagaaatata gactgtaaaa tatgggcagt
     3121 tggagaagga gatataaaat cggagcttga taactttgat attttacttc ttggaccaca
     3181 acttagattt atgcttgatg acgtaaagtc tatagttgga gatagagctc cagtatctat
     3241 aatagatatg gtaaactatg gcacttgtaa tggtcatgct gtgttaaatt cggttttaga
     3301 aatattaaaa taaataataa aagtggattc tatataatag agtccatttt tattatttgt
     3361 tattaatagg cttaaaactt agcttaccat ttcttttttt aatctccaca tagcaagtga
     3421 aaaacttgaa cagcacaata ttaaaaatag taggataaca ggcattatat ctccaataag
     3481 aatagtctca ccaaatatta agctacgtaa agcgttaata acatgggtaa atggattgag
     3541 catcactgct acttttaatc ctccagataa actttctata gggaaaagag ctgaacttaa
     3601 aaagaaaata ggaagtacaa ttgcagtcat aattgtttca taaactattt catttggcaa
     3661 taataaactt atagaataag ctagacttga taaaaagaag gctgtcataa atatcagtac
     3721 tatcataagt aggattccac caatgccaga ttctatcctt actgagaaga atatacttac
     3781 tatacacaaa atagttactt ctatgaaaga aactaagata gcttctaata attgaccaag
     3841 gacgattgaa tacctgctga taggtgcaat taagactcta tagaaacttc cactgttctt
     3901 cattataaag tttataatac caccactact acaagaagaa aacacaacca gtacaatgat
     3961 acctggtagt ataaaagcag tataattatt gatatttata ttattcatag tttggtttgc
     4021 aatagaacta taaagcacaa gccaaagaaa aggttgtaat atagtaacta caaatgagat
     4081 tggattttta aaacgccact tcatactacg ccataagata tttaacattc ccattcctcc
     4141 tttaagcttt ttttagaact tgtaagtgct aaaaatacat cttcgagact tggctctaca
     4201 atttctattg catcaaattc tatatcatgt tctaaaagcc atttatttat taaagtaaaa
     4261 gcagttctac tatcgtttac acttataaaa attgaatttt ctcgtacgct cataaacttt
     4321 actaaaccag tacttttaat tgaatcttta tattttttta tatcttcagt attatgaaaa
     4381 gttatacgta gtatattttg tctaatgtaa ctacgtaaac ttgatggtgt tccttgtgct
     4441 aaatcctttc cattcttcat aatacaaata ttatcactca attgttcagc ttcttcaaga
     4501 taatgagtag ttagaaatat agttgtgcca tactcatctc taatttttaa taacatatcc
     4561 cacatagatt ttcttgaatc gacatccata ccaacagttg gttcatctaa aaataggatt
     4621 ttaggagatg ataccatatt catagctatg tccaatctac gttttacacc ccctgaatat
     4681 gatgaagtag gatattttaa gtaactagat aagtcaaact tatcaattaa agagtctatt
     4741 ctttgttttg caatctgtgg ttctacttta tacattttac tttgaaatac catattttcc
     4801 ataagggata aatgttcatc aatagagatt tgctgtgcta cacaagcaat ttgagtacgt
     4861 atccaagaag gattatctac taaatctttt ccaaacattg ttacatttcc agatgtaggt
     4921 ttgtaaaagg tagtcaaaat atttataaga gaagatttac cagcaccatt aggaccaagt
     4981 aaagaaaaaa tttctccagc atttacatta aagcttagac catttaaagc tttcacacca
     5041 tttttatatt cttttactaa attgtctatt tcaatcgcta acatatatta ttcctcctct
     5101 ttaagtggaa atattatctc tgtaatatag ttatctgggt ttccttttat caacatacct
     5161 ggtcctttaa taaatacttc tctccaagga gtttgtattt ttatattatt ttcatgaata
     5221 tatttaaata tcatttcata cgtttttggc aaattactat aagagcctgt gtgagtaaaa
     5281 aataaagctt ttgttttagg aaattctttt gtagttatac ccatacgatt tggagtttca
     5341 gcagttggaa cacataattc catttctgca acaccagttt tttggtcaac atcatagtag
     5401 caaaagaatg gtgcaccgtt agactttcct tttatagctt taaatacatt tggaaaatac
     5461 tttgttgcct cagtcatagg tccattataa cgcatggtag ccactgtgac tgattcgata
     5521 tttttgattt caattttgca attcatttgt ttacctccta atgtgtgttc atattcttac
     5581 tagattttta agcattaata catataagat tttgattagt tgaaaacata taatcttatc
     5641 tcgtttgctt gttttcaagt ctaacataac ataaaatata tatcctctca tttgatgctc
     5701 cattttgtaa agttattatt taagctttac tttagatttt ttagcaaatt aaaataaagc
     5761 aatttataaa ataagtattt acaaattaag tgtattaatc gtataataca cttaatagga
     5821 tactagataa agtctgaaat aaaggcgtat ttttttaaag taagtgtact atcgtattaa
     5881 tacactagaa taggaggaat catgaatttt gaattagata atacaactcc catttatttg
     5941 caaatagtaa agtatataaa gagacaaata gtaacaggag agttgaaacc aggagagaca
     6001 ataccatcaa gaagagaaat ggcacttaat ctaaaagtaa acttaaatac agttcaaaga
     6061 gcatataagg agatgggtga tatgaatata ataaatactt ttaaaaatta tcaaagcagt
     6121 gtaacagttg atgaaaatat attaaaaaat ttaaaattag agcttataaa tgaatcacta
     6181 tctgttttta tagaagatat gaaagcaata aatgttagca aagaagaagt tttaaaaata
     6241 atagaagata agtattagtt ttttaacaaa tagaaaactt agtttagaaa gtttatttat
     6301 aagcatattg gatgtggagg atagtaaata tgatagaaat aaagaatgtc agcaaaacat
     6361 ataaaaggat gcaagggctt aaaataagaa agatagaagc tttaaaaaat gtaagtttta
     6421 atatagaaaa gggaaaaata actgcattac ttggtataaa tggtgttggt aaatcaacta
     6481 tgttaaaagc aatagcagga cttataaaca ttgatagtgg agaaattcgt atagatggag
     6541 aaaaaataaa tgaaaaggta tacaataaat tggcttttgt tccagatgta caatctcact
     6601 ttagcaatac aacaataaaa gaaacatttg aatttatgga aattttttat tcaaagtgga
     6661 ataaagaaaa atcaaaagaa atgatggaca tatttaaatt ggatgaagat gaaataattg
     6721 ataacttgtc aaaaggaaac attgcaagag taaaattaat acttggattt tgccaagacc
     6781 cagagtacat acttcttgat gaaccattta caggaattga tttgtttaag agggaagagt
     6841 ttataggagt aatagcgcaa tatatggagg aaaatcaagc cattattata actactcatg
     6901 agatagtaga gatagaaagt ttagttgatg aggttgtaat tcttgatgaa gggcagatta
     6961 taacttcatt taatgcagaa gaattaagag aaagagaagg taaatctata ttagataaga
     7021 tgagggaggt atataagaat gaataaactt ctatatctaa taaatgtaga tttgagaaga
     7081 agcaataagt tttatatagc ttatatatca tttttttcat taattacact tagtttaaat
     7141 ttattcgaaa ttaataaatt taaaaattct aaatttatta tgaacttagc aatagaagat
     7201 tttggaggta tattttatgg actagggata cttagtaatc ttggttttat ccaaagtata
     7261 ttgttctttg ggatattggg actttttata tactatattt ttatgtggaa aagagagttc
     7321 ttttcaaata atcaaagtat ttatacactt atgatgttac ctcaaaataa gtttaagatt
     7381 tgtatatcta aatcaatagc cttgataaca atgatatatg gttttttgat aagtcagaca
     7441 gcaacattat ttatatgtaa atatatattt aatttcattt ttagaaatat gcctataatc
     7501 aatatgaact ttgccaaaga tttatattat ttaaacttga agagtatacc aattgatttt
     7561 atctctttta tggctattta tgtattttta ctacttatag ctatttctat tgtaaattgc
     7621 tgtatattat ttatcttttc ctatataaat aaattttatg taattttaat cactttgctg
     7681 attatagcag gcttatattt ttcaacaata tatataaa
//
LOCUS       DAYAOX010000172         7683 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.173, whole genome shotgun sequence.
ACCESSION   DAYAOX010000172 DAYAOX010000000
VERSION     DAYAOX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.173"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            51..1109
                     /locus_tag="ACGI57_002872"
     CDS             51..1109
                     /locus_tag="ACGI57_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HFG9717936.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRSIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1213..2397
                     /locus_tag="ACGI57_002873"
     CDS             1213..2397
                     /locus_tag="ACGI57_002873"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9717937.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMIVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2604..3317
                     /gene="rpsB"
                     /locus_tag="ACGI57_002874"
     CDS             2604..3317
                     /gene="rpsB"
                     /locus_tag="ACGI57_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFG9717938.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3396..4307
                     /gene="tsf"
                     /locus_tag="ACGI57_002875"
     CDS             3396..4307
                     /gene="tsf"
                     /locus_tag="ACGI57_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFG9717939.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4392..5096
                     /gene="pyrH"
                     /locus_tag="ACGI57_002876"
     CDS             4392..5096
                     /gene="pyrH"
                     /locus_tag="ACGI57_002876"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFG9717940.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5122..5679
                     /gene="frr"
                     /locus_tag="ACGI57_002877"
     CDS             5122..5679
                     /gene="frr"
                     /locus_tag="ACGI57_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFG9717941.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDALLSKKEKDIMEV"
     gene            5682..5885
                     /locus_tag="ACGI57_002878"
     CDS             5682..5885
                     /locus_tag="ACGI57_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717942.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5914..6648
                     /locus_tag="ACGI57_002879"
     CDS             5914..6648
                     /locus_tag="ACGI57_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFG9717943.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6661..7440
                     /locus_tag="ACGI57_002880"
     CDS             6661..7440
                     /locus_tag="ACGI57_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFG9717944.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3167 a          782 c         1457 g         2277 t
ORIGIN      
        1 aaactaatac aaaatagtaa taattgtttt gtactggagg atatatattt atgaattgta
       61 aatggatatc taaaatagat gagagaaaaa aatgtcatag agaagcagat tcttctggat
      121 attgtatctt tcataaagaa aataagtctg atgaagaaat acagttgatg atggatacta
      181 ttcataaaga agagataagt gaatttaatg gatttgtttt tgaaaatgaa tttaatgcgg
      241 aagaaatatt aacttacaat tataaaatac tggatttttc tgaatccata tttaaacaaa
      301 aagcaaattt taaaaaatat atttttaaaa agaatattat ttttaactat acagaattta
      361 gagataaagt tttatttaat ggttgtgtat tcttagaaaa ctgtgatttt aatagaagca
      421 tatttagtaa aaactatata aatgaccgta tatttgaaaa agtgaaattt aaaggacctg
      481 atttggtagt taataaagta gaaaattttc caagaatgga tggaataatt tttagcatgt
      541 gtacaaaatt tgtgttaaag aatgttgaat atggaaaaag tgagtatgag catggcaaga
      601 taaattatag aatagctaga aatcaagcaa caaaaattgg agaatatgag atgataggat
      661 tttactatta taaggaaaga atttatagta gtaaaataat gaaacgttct aattatccta
      721 ctttcagtga ttatttagtt gaaaaatttt ttgaccaaat agcacgttac acaacaggtt
      781 atggagaaaa gccatggaat atattattag ttattatagc aataataagt gtttttgctt
      841 tattatactt atttgtaggc atagaaagtt caaatagcac acttgtagct ttagatataa
      901 ataatatagg tgattactca ctaagtgaga ttttcaaaat gtatatggat ttatggtatt
      961 ttagcatggc tacatttagt acagtaggat atggagatat ggttgctaca agtttgattg
     1021 ggaaagcatt ggcaggtata gaagtatttt ttggagttac aataggagca atttgggcat
     1081 ctgtgattat taaaagaatg ataaggtaat atttgtgtaa atatggatag gtattaatta
     1141 ctagatataa attggactaa atagggaaaa tttaaatata aatatactta ataatccaag
     1201 aaggtgaaaa atttgaaaaa tgcactaaaa aaaattatat cattagccat gattctagtt
     1261 tttataatac caaattattc atatgcagat gataaatctg tagacaagta ttcaaaaaca
     1321 tctatactta tagaccaaga aacaggtaga gttttatata gtaaagaacc tgatatgaag
     1381 gtaccattag ctagtttaag taagatgatg acttttttac ttgcaataga agctattgaa
     1441 aataaagaag taaaagaaac tgataaagta aaaatagaca aatctattgc atcagtaaaa
     1501 gggtcaagtt ataaacttaa agatggtgaa gaagtgccac ttatagagct tatgagaggt
     1561 cttatgattg tatcaggaaa tgatgcggca attgctatag caaaacatat ttgtaaaacg
     1621 aaagaagcat ttgtagatag aatgaataaa aaagcaaaag aaataggaat gagtaataca
     1681 cattttctaa atccaaatgg tcttccaata tacgatttaa gcaaccccaa aaaaccagca
     1741 aaagaaaata tctctactgc aaaagatata gctattcttg gaaaatacat gtttgaccat
     1801 tatgaaaaac aagtaacagc tataactgat atggagacat atacaaattc agatagaagt
     1861 tttgaaaaaa gcaatacaaa tgcattatta agaattatac cagaagtaga tggtataaaa
     1921 actggatata ctggcaatgc tggatattgc ttatcatttt ctatgattgt aaataaaaat
     1981 gataaaaatg agaaaaaacg tagagtaata ggggttgtat taggtactaa ccataaaaat
     2041 aaaagaacct ctgcttcatt agctctactg aaatatggaa aagaaaattt caatatgaaa
     2101 aaagtaattg ataaagatag ctttattggt aagaaatata taaaaggaat gaaagagtta
     2161 gaggttacat taaaagcaaa agatgaactt tatacaatat taaaagatga tgaaagtata
     2221 aaatcagaag ttaagattaa aaatatagag taccctgtta aaaaaggtga tactatgggt
     2281 attataaaat attatactaa ttcaggtgac ttacttggaa gtgtagatat agtaagtaat
     2341 aatagtatag ataacgtatc attaaaaaca aaattaaaaa tgaaatttgc aaattaaatt
     2401 caaaaaatgg tataaaatac acaaaaaaat ataaaaatag aaaatatgga aaaaatattg
     2461 ttatttcttc acctctgtga tatactacta gaggtgaaaa aataaaataa atcacacaca
     2521 gcattgggat ccttaaaagg tgcctattac atggtttttt agggagaaga tcaagctgga
     2581 ggtataaaac caggaggtaa gatatgtcag taatatcaat gaaacaatta ttagaagcag
     2641 gtgttcactt tggtcaccaa acaagaagat ggaaccctaa aatggctaag tatatattca
     2701 cagagagaaa tggaatatat ataatcgact tacaaaaaac agttaaaaaa gttgaagaag
     2761 cttacaagtt cactaaagaa gtagctgaaa caggaaagcc aatcttattc gtaggaacta
     2821 aaaaacaagc tcaagatgcg ataaaagatg aagctgaaag atgtggaatg tactttgtta
     2881 atgaaagatg gttaggtgga atgttaacaa accacaaaac tataaaaaca agaataaata
     2941 aattaagaga attagaaaaa atggaagaag aaggcgtatt taatgttctt cctaaaaaag
     3001 aagttataaa attaagagct gaaaaagaaa aactagaaaa atacttaggt ggaataaaag
     3061 acatgcctga attaccaggt gcaatgttcg tagttgaccc aagaaaagaa aacatagcta
     3121 ttcaagaagc tcatagatta ggtataccag tagttggtat agttgatact aactgtgacc
     3181 cagaacaatt agactttgct ataccaggaa atgatgacgc tataagagcc gtaaaattaa
     3241 taactggtgc tatggcaaca gctgtaattg aaggtagaca aggtgctgag gaagaagtag
     3301 ctgaagacca agaataataa tttgacaatt aaatggtaag ggtcgccctt accatttata
     3361 atatggaata gaataagtag gaggaaactt aaaaaatggc taatataact gctcaaatgg
     3421 taaaagagtt aagagaaagt acaggcgcag gaatgatgga ctgcaagaaa gctttacaag
     3481 aagctgaagg aaacatggaa aaagcagtag atttattaag agaaaaagga ttatcaaaag
     3541 ctgctaaaaa agctggtaga gttgctgctg aaggtctagt tgcaatagaa atgaatgatg
     3601 ataatacagt tgcttctatg gtagaagtta actcagagac agatttcgtt gctaaaaacg
     3661 aagacttcaa agtgttcgtt aaagatgctg cttgtatggc attagctact gataaagaag
     3721 atatagcttc tttattaggt gaaactcata aagagggaat aactttacaa gaagttttaa
     3781 ataatagagt tgcaaaaata ggtgagaaat tagactttag aagatttgct aaagttgtaa
     3841 ctaatggaca agttgctgga tacattcacg gtggtggaaa aataggtgtt cttgttgaaa
     3901 tggaaacaga agctagagat gctaaagttt tagaattagg taaagatgta gctatgcaag
     3961 ttgctgctat gaatcctaag tatgtttcaa gagatgaagt agatgctgaa tacatagcac
     4021 atgaaacaga agtattaact caacaagctt taaatgaagg aaaaccagct aatatagttg
     4081 aaaaaatggt taaaggaaga ttagaaaaag aattaaaaga ggtttgttta ttagagcaaa
     4141 ctttcgttaa aaatccagat ataactgtta aacaattagt tgctgatgtt gctaaggcag
     4201 taggttctga tataaaagtt gttaaagttg tgagattcga agttggtgaa ggtatacaaa
     4261 agagagaaga aaactttgca gaagaagttg ctaagcaact taagtaataa ccaaagagag
     4321 aacacgcagg tgttctcttt tttaaaaaat agaaaaaatt ggatatgaag gattgaaata
     4381 ggaggtttct gatggataaa ccaatgtaca aaagggtatt gttaaaatta agtggagaag
     4441 cattagcagg agaaagaggt tttggtataa ataatgatgt agttaatgat attgctattg
     4501 ctataaaaaa aatacaagaa ataggtgttg aagtagctgt agtagtaggt ggaggaaact
     4561 tctggagagg cagaaccagt gaaggaatgg acagaactac tgctgactat ataggaatgt
     4621 tagctacagt tatgaatgca atggcgctac aggatgcact agaaaatata gatgttgcta
     4681 caagagttca aactgctata gacatgagac aaattgcaga accatatata agaagaagag
     4741 cagttagaca cttagaaaag gaaagagttg ttatatttgg tgcaggaaca ggaaaccctt
     4801 actttacaac agatacaact gctgctttac gtgctgctga gatggaagca gaagttatat
     4861 tgttagctaa aaatgttgat gcagtatatg acaaggatcc aaaagtacat gcagatgcta
     4921 agaaatttac agaattaagt tacatggaag ttatacagaa agaattaaaa gtaatggatt
     4981 ctacagctac atctttatgt atggataata aaattccaat aaaggtattt gagcttacaa
     5041 cagagaatat aataagagca gtaaaaggcg aaaatatagg aactactgta aaataatgaa
     5101 gaatgaagga ggagtaaaat tatgaaactg gaaatacata agcaattaga agagaaaatg
     5161 aatgggacaa ttgatgcttt aaagtttgaa tttggaacta taagagcagg aagagctaat
     5221 gcacaaatgc tagacaaaat aagagttgat tattatggaa ctccaactcc aataaatcaa
     5281 ataggtgcta tatctgtacc agaaccaaga atattaatga tatctccttg ggataaatct
     5341 gctatgcatg aaatagaaaa agcgatagct aactcagact taggattaaa tccatcaaat
     5401 gatggtgagg ttataagact ttctgtacct gctttaacag aagaaagaag aaaagaatta
     5461 gctaaaaaag ctagtaaagc tgcagaagaa tttaaagtta gaataagaaa tgaaagaaga
     5521 gacgccaatg agaaaattaa aaagatggaa aaaggcggag aattaactga agatgaatta
     5581 aaaaaagcac aagatgaagt tcaaaaaatg actgataaat ttataaaaga aatagatgca
     5641 ttattatcta aaaaagaaaa ggacataatg gaggtataat attgaaaaaa tattataacc
     5701 ttttaggtct tcatgtagat gaggtaaaac aatattttga tgagcaaaat ataaattata
     5761 ccgtaaaatc tattgaaggt aaaaaggata aagaaaaact tgtagttcct agagttataa
     5821 aaatatctga gataggtgat agtgtagaac tgataatcac atacttttct gactccttag
     5881 tttaaggagt cagaacaata ttggaggcaa aatatgaata acaacattat atatgatata
     5941 gacttaaata acatacctac tcatattgct attataatgg atggaaatgg aagatgggca
     6001 aaagctagat ttcttccaag aactgcagga cataaggctg gagtagaaac tataagagat
     6061 atagtaaaag aatgctcaaa attaggtgtt aagcacttaa cattatatgc attttcaaca
     6121 gaaaactgga aaagaccgaa acttgaagtt gacacactta tgaacttatt atctacatac
     6181 ttaagaaatg aaatagctga acttcaccaa aacaatgtaa aagtaactgc tataggagat
     6241 ataagtgctt taccaaaaac ttgtataaga gaattaaact cagctaagga actgacaaaa
     6301 aacaatacag gagttaactt aaatttagca ttaaactacg gaagtagagc agatataaaa
     6361 aatgctctta tagatatagt aaaaaattgt gaaagtggta aaatagatat aaacaatatt
     6421 gatgaagata ttataaaaaa ctatttaagc acaaagtcta taccagatcc agacttggta
     6481 ataagaacaa gtggtgaaca aagattgagt aactttttac tttgggaagt tgcttattct
     6541 gagttttact ttactgatat acattggcct gattttaaaa aagaagaact acagaaggct
     6601 atatacgtat atcaaagaag ggatagaaga tttggaggac ttaagtaagg agattatttt
     6661 atgcttacaa gaattattgc ttcactagct ctggttccat tatttctatt tgttgtatat
     6721 ggaggtattc ctctatatat agctgagaca gcaatagttt atattgcatt gcatgagttt
     6781 tataaggcat ttaaaataaa agatgtacat ccaatattca ttataggata tttattttct
     6841 atttatttag ctgtaaaaaa tatttttaat ttaccattag aatatactta tgcagtaatt
     6901 tttattttat ttttagcaag tataatttat atgttgatgg gcaaaaacaa tgttatagat
     6961 gtttcgatta cgtttttagg agttttttat ataggagtat ttttagattt tataattata
     7021 actataaatg gatttgaaaa aggtagtata tatgtatggc ttatatttgt gatatctttt
     7081 atgacagata tatttgcata ttttagtggt tatttacttg gaaaacataa gctaattcct
     7141 aaagttagtc ctaaaaagac tatagaagga gctattggag gaataatagg aagtacactt
     7201 tgttgtattt tatttggata tttatttggc attgatttgc ttcaattagc gataatagga
     7261 agtataggaa gtgtaatagc tcaattagga gatttatttg catcttctat aaagagatat
     7321 gtagggataa aggattatgg aaagataata cctggtcatg ggggtatatt agatagattt
     7381 gatagtgtta tattagttgc accttttgta tatagtgcga ttaaattttt tattagataa
     7441 agattggttg gatttaaaag agcaatctat aattgctagt aatgaaaata gctatgtaac
     7501 ttttaaatgc agagatattc tgcagttatt agttacatgg ctattttcat tgttcaaaaa
     7561 tttagaagac cttggggaat tgtataagaa taggtattac tttataatag tatactatat
     7621 ttgtagtgag tatatttttt atactgtaac tatcattaaa aaatattttt ttgtttaata
     7681 att
//
LOCUS       DAYAOX010000173         7631 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.174, whole genome shotgun sequence.
ACCESSION   DAYAOX010000173 DAYAOX010000000
VERSION     DAYAOX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.174"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            344..1054
                     /locus_tag="ACGI57_002881"
     CDS             344..1054
                     /locus_tag="ACGI57_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9717945.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            1048..2364
                     /locus_tag="ACGI57_002882"
     CDS             1048..2364
                     /locus_tag="ACGI57_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9717946.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            2376..2516
                     /locus_tag="ACGI57_002883"
     CDS             2376..2516
                     /locus_tag="ACGI57_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HFG9717947.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            2575..3156
                     /locus_tag="ACGI57_002884"
     CDS             2575..3156
                     /locus_tag="ACGI57_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="HFG9717948.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            3303..5012
                     /locus_tag="ACGI57_002885"
     CDS             3303..5012
                     /locus_tag="ACGI57_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717949.1"
                     /translation="MSSIKAISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKINSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            5027..5899
                     /locus_tag="ACGI57_002886"
     CDS             5027..5899
                     /locus_tag="ACGI57_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9717950.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLIDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALICDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            5900..6688
                     /locus_tag="ACGI57_002887"
     CDS             5900..6688
                     /locus_tag="ACGI57_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717951.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVSYIARKSTVLKKSK"
     gene            complement(6784..7617)
                     /locus_tag="ACGI57_002888"
     CDS             complement(6784..7617)
                     /locus_tag="ACGI57_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HFG9717952.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNCA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
BASE COUNT         3125 a          711 c         1096 g         2699 t
ORIGIN      
        1 ttaatattta aaatatagtg tgtattcttc aaaattatag cgataattaa aatattacta
       61 taaaaaggaa ggtgcatact ttttatttaa caaaaactag atattaaaaa cttttcgagg
      121 aatattttat taaaatccct aaatatattt aatatatatt aaaaaattgt attataatga
      181 aaatcaaaat agattgcaaa tccaaaaagt aatacttaat gtccaaaaag ctaaaattaa
      241 aatctaaata gctatttact acagaagtat gttaaaatat gtaaaatgaa tagaaattta
      301 tatattttta ttaaaaatat ataagaaagg atgtttaaga attgtgatta atataggaat
      361 atgtgatgat gaactacatt atcgaataaa aataaaagat atattaagtg aaatattaag
      421 ttcatatcct ataaattata acatatatga attttcagca ggagaagagt tactaaataa
      481 ctatcctaaa gatttagaca tattaattat ggacatacaa atgaaaacaa taaatggtat
      541 ggatactgct aggaagataa gagagtttga tcataagtta gaaataatat ttgttacttc
      601 atttgtagag tttatgcaag aaggttatga ggtaaaagca tacagatata tactaaagcc
      661 aataaataaa gaaaaaattt ctaaaagtgt attaccttgt ataaatgaga tgatgaaaaa
      721 gagaaataat tacttaacta taaatgtaaa aaattatgta gaccgaatta aaatagattc
      781 tattacttat atagaaacag atagaccgaa tatattgatt tatacacatg atgatatgta
      841 tattactaaa atgagtatat ctaaaataga aaagatatta aatgaatatg gattttttag
      901 atgtcataat agctatatag ttaacttaaa attagtagaa tcaatgagtg gaagtactgt
      961 tatagtagat ggaaaaagta ttccaataag taaatatagg gttaaaggct tgaagcttgc
     1021 gataactaat atattagggg acatagtatg ctaacaaatt taagtgtagg attttggaat
     1081 attattaatt tagtttcagg tactatagat tggattatat tttttttagt aatccactta
     1141 gttggaaaga ggaaaatatc taatataaga tatatattta caacaataat tataattata
     1201 tttatggcat acataaatgt agttaaagtg tttccaaata ttaagattct tctatgtatg
     1261 gtaataggaa tatcatttta tataacatca tatgaagata aaatctataa aagtataata
     1321 gttagtttat tgttttggct aggattaatg gttgctgaag gttctgcagt aggtctagta
     1381 gtatttataa ataaattaaa taatattaac ataacacaac atgaaaactt atttagaata
     1441 caagctatta ttatgtcaaa ggtattttta tttataggtt ttatattgtt taagtatttt
     1501 aaattatctt tagattttaa acctaaagat atgatactaa taggaatacc tatattatct
     1561 aatatagtta gcttattact tatatttgaa tataatttaa gagtcaatac aattactaca
     1621 gaaaacttat ttatatttat atttgcaatt ttattgattt tatcatcaag tataatactt
     1681 ttaatagtaa tagggaaaat tgtacaagac gataaattaa atctagagta tgaacttata
     1741 aatgaaagaa taaaaacaaa ctacaggaat tatgaaaata taaacgaagt tcatgataga
     1801 ttaaaatatg tttatcatga cttgaaaaac catatgattt gtataaaaaa ttatgacact
     1861 aaagaagaaa ttatatcata tataaataac ttagagtttc agataagtga atttgaacat
     1921 tttaaaaata ctggaaataa aacattagat ttaattttag cagaaaaaat aagtatttgt
     1981 aaaaagtatg atattcaaat tgaagataac ataaatatat ctaaattaaa ctttattaaa
     2041 aataatgata tatgttcaat atttgcaaat tcactagata atgcaattga agcatgtatg
     2101 gatataaata atgagataga aaaaagaata gaagttaaag ctacatatat aaataaattt
     2161 gcaataataa aatttataaa tactaaggta aatgatatta aacttattga taaacgtatt
     2221 caaactagta aggatgatga taagatacat ggaataggac ttgctagtat taaatatata
     2281 gtaaataaat atggtggtga aacaatagtt aattactcag ataatgagtt tatattaaaa
     2341 ataatgatac caataaaaag ttaggagagt acaaaatgaa aaaaatagcg ttaaacttac
     2401 ttaaaaacat ctcagcatta agttttggga tagctgtatt atcagcaaac agtgcaagtt
     2461 cttgggtagc acatcaggca aaagagccac aagctttaca aaaactaaaa aaataacatt
     2521 aataaaagga cctcttctta ttaaaggaga ggttttacat gttgaggagg aaatatgttt
     2581 aaaagattat catataaatt tgccaatata ttagttaata atgagattgt agaaagtgaa
     2641 gattttgaaa tatatagata tggatttgaa actttgattt atttcatagt taatattttg
     2701 gtagcattat ttataggaat tatttttgat agatttatac atacagttat atttttaagt
     2761 tgttattgca ctttaagaca gttcacagga ggttatcatg ctaggaatta taaagagtgt
     2821 acattaacat ttgcagttat ttatttgata actatttttt cagctaataa tatagatata
     2881 aataaatata agtatttatt agtattatta atgattatta gtatactaac aatttacaag
     2941 ttagctcctt tagaacatag aaacaaacct cttagtgaaa gtgagaaaaa gcattataga
     3001 aagactgtgc aaaaaatact attcgtaata atatgtttaa ttattttatg taaaatactt
     3061 aatatttttc agcaatatgt gatttatgca ataatttcta tatattggat tgctatatta
     3121 atatatattg gaatgaaggt taatagtgat cagtaagatt ataaaacatt ttctaaatta
     3181 attaacttca ctaattaata ttaactttaa aaaaataatg tcaaggtaag tatttgaact
     3241 tattaaatat agttgaatct attgtaggtt gtatcaaaag actggctagg aggggcataa
     3301 atatgagtag tattaaggct atatcagact ttataaccaa aataattaga aaaaatattt
     3361 tatttgcaat aacaattatt attataaatt accatctcat acaacgatgg acacatatat
     3421 ccaatagagg aatttcattt aatgattact tatccttgag tgtatatact tgtatgacaa
     3481 ttagtatttt aaccatgtta aatgtaattt tttatatgag aaaaagttat gatactgtaa
     3541 aaatattatg tagaaataat tttgattatt ttttaggtat ttctgttgct gtaactagaa
     3601 acaactttat attttgttgc ataccaatta tatatgctat agcatactca agtttaataa
     3661 gaacattgtc aattgtaacg attaaatcaa ctatatttat attgatattt gaatggtttt
     3721 taccacttac tattttatct atagtgatat catttatttc agttattata aataatttaa
     3781 tactaaagat taccttatca tcagtatttt tatatttaac atcaactaat atgttggata
     3841 gagttattga tattaaagaa ccatttttta aagcattatt taaatcactt aacatatttg
     3901 aagatcaatc atatgcttac tattctgatt ttgtagggaa agttaataat tcagcatatg
     3961 ttatagataa gattatacct acactatttg caattacttt aataatagta gcatatctga
     4021 tattaaatga tattagaaag aaacagatta aatcattaat agttatagta gtgtacagta
     4081 ttttatttat tggattaaat tatatatcaa tagatatgtc aacatataga gatgaggagc
     4141 gtagttatgg agagtattta aagaaatcta atattagtga tattaaaata aaatcgcatg
     4201 atatgaagat tgactttaaa agcaagtcaa atataaaaga tagaattgtt ttattaaaca
     4261 catcaaatga agagttaaca aatattaata tgattttaga tgaaatattt gatataaaat
     4321 atataaaaat aaattcggaa aatataaaat ttaacaaaaa aaaggattta attgaagtta
     4381 atttaaagga aagtttagag cctaatgacg aggtgacatt agatatagag tatgaaggct
     4441 atattaatgt gttgacatct tggaatcaaa ataaatatat agctactaaa gatgatatta
     4501 tgcttccacc aggttcttta gcgtggtacc caaaagtaaa tcaatcaagt tgtatggctt
     4561 ataaaataaa tttaagttct aaaaataatg tctattcaaa tttaccttta ctgaataaga
     4621 ctagtaattt tttaaaaaaa gattatatat ttgaaggaaa gtcaaatgat attgcaatat
     4681 atgcaggtga gtttaaagaa gaggtaaaag atgatataaa aataatctat ccatcagata
     4741 gtaatgtaaa tatggatata gaggaaaaaa ttaaattttt aattgaaaca agaaatgaaa
     4801 tattaggtga tagcaaagca tctgtagatg ctaaaaaata tgtagatgaa gtagataatg
     4861 ttatggaatc aataaaaaga aaaaaaatta atatgatatt agttgctgac ttacctagca
     4921 atgaattttt atatgaagac tcaaagggag agaaaattag cataactatt tatcattggc
     4981 tttcaggaaa tatccttata atagataaat aataaaggag gttaatatga taaacaagtt
     5041 agaaataaga aatctaaatt ttaattacaa aaataaaaaa gctttggata atataaattt
     5101 aaccctaata gatggagtag ttgcattatt aggtcctaat ggtgctggaa aatcaacatt
     5161 aatgaggtta ttagtgactc tttatgaaac ttcaattgga gagatagaat taaataatat
     5221 aaagtatagc aaaaacaatg agaaaatcaa ggcaaatgta ggatatgtac ctcaagattt
     5281 tgatatgtat aataatataa atgggcaaga atacttagaa tttgtagcta aaatgagagg
     5341 tgtatctaag aatgatttaa agaaacatat acaaaaagta gtatcaaaag ttaatctgga
     5401 caagtttata aataaaaaaa taggtacata ctcaggtggt gtaaagagac gactaggtat
     5461 agcacaggct ttaatatgtg attctaagtt aatagttatg gacgaaccta cagttggtct
     5521 tgatccagag caaaggaatg aatttagaag attgttacca gaaataagta aaaattctat
     5581 agtattaatt tcaactcaca tagtagaaga catacaattt aactgtgaca aattaattat
     5641 attaaatcaa ggcaaaatat tatatgatgg tacaattaat aagtttattg atatggttga
     5701 ggtgtattcg gtagtagttt ctaatgatga atttgaatat ttagaacgta atattgatat
     5761 tattgattat aggagagtta aagatggtgt gcaagtaaag tatataggta aaaatacaga
     5821 gaaacttaaa gatagcttaa ggatagatac aactttacaa gatgcttata taaacttttt
     5881 acataaggag ttaaaataaa tgtgtatatt ggagttaata aaatttgagc ttaaaaaaat
     5941 agagcttaaa catgtgttaa tatttgtaat tatttctatc ttatgcatat ttattacagg
     6001 catattagat tataatgagt taatatttta taatgatgta cctataatgg atacatattc
     6061 tatagcagga tggttaggaa ttataaagat atcagatgtt tttataccaa taatgtcagc
     6121 aatcacaata atttatgtgt tttcaaaaga ttattcgggt aatataaatg agatacttac
     6181 tttatataat aaaaaaaaat ataatcaatt tatagtattt agatggtgtg ttatattagc
     6241 tatatatact gtattattat taatttcaat tacaatagta tatacattta cgtcagtgaa
     6301 aggttttaac atagacaaaa tagattttgt ttatagtgta aagccaatag atgtgttttt
     6361 aaagacattt cctacattat tatggtatac aacatttcca ctaatgatat taacattaac
     6421 aaaaaataga tttacaacaa tagcaatatt aattacatat acatttgctg atatattttt
     6481 tattttacat atgtacccat ttgttagtat gattaatgta aatggatttt atattcaaaa
     6541 gatgttttat atgcctaaca atatagatgt tagatttaat gaattaaaga attatttctt
     6601 tataaataga ggttctctag tagttatttc tataataata gtttcttata tagcaagaaa
     6661 atcaacggtt ttaaaaaaga gtaaatagtt taatatattt tttataatgt tatatttata
     6721 tagaatagtg actttaaaaa atttaaagcc actatttttt aatatgtttt atctatataa
     6781 ctattatttt tctaggtttt ttaatatttc gtaatagtgt gttgtggcta tttcacttga
     6841 ctcgcctttt ttacaaaaag ataatttaga tagttttgga actacacgtt caacgacaat
     6901 tttattacct atcattttag attcaagtat cttcaatgct tctaaaaatc tattatcctt
     6961 ttttgcacaa ttataaaatg ataaaacgta gacataaagg aataggttat aattacggaa
     7021 aggatattca acttgcataa ataaggttcc aattccatag tgacaagggc cgattggttt
     7081 tttaatagtc caatggtcaa gtagaaaatc tacagctttg tcaagttttg attctttatt
     7141 tagataatta ctaaaacgga atgcatttaa tatattaaga gttggtagtg gatttgagta
     7201 ttcagtttct ggacctcgac caaaactaaa tttattgcaa cgccacccac catcagtata
     7261 ctgtgtatct aaaaaatgtt gaaacgtttt ttgcattcta atgtcagatg catatcccat
     7321 atgacagagt acgttagcag catttgcagt atgacaaggg taaatacctc catttggata
     7381 tagtttgaaa cgtccatctt ctcgccatgt gctaaatatc aagtcagcac attctttaag
     7441 taaagtatca tcagggttca cacctaattc taatagataa agtacactat caagagtaga
     7501 gaaaggagaa ccttttatta aacgcttatc aggtgtagtc caatagtctg caccattatc
     7561 atacctgtgt gataatatta tttcaatatc tgataaagat tgattttcta cattcatttt
     7621 ttactccatt t
//
LOCUS       DAYAOX010000174         7628 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.175, whole genome shotgun sequence.
ACCESSION   DAYAOX010000174 DAYAOX010000000
VERSION     DAYAOX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.175"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            91..1365
                     /locus_tag="ACGI57_002889"
     CDS             91..1365
                     /locus_tag="ACGI57_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HFG9717953.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(1526..4567)
                     /locus_tag="ACGI57_002890"
     CDS             complement(1526..4567)
                     /locus_tag="ACGI57_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HFG9717954.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKIDDNNISNSVKEAWKKRNGKDYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            4773..5072
                     /locus_tag="ACGI57_002891"
     CDS             4773..5072
                     /locus_tag="ACGI57_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFG9717955.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(5311..7539)
                     /locus_tag="ACGI57_002892"
     CDS             complement(5311..7539)
                     /locus_tag="ACGI57_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFG9717956.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT         2640 a         1096 c         1077 g         2815 t
ORIGIN      
        1 ctatttagaa agaattggta ttatataaag taagagaata tataataaga aagaatattt
       61 gattttattg gaaaacaaaa gaaaaagaag gtgattacta tttctcatat aactgcgaag
      121 aatatgtata aaagtttaga ggaaagaata aacaagtttc cacaaggtgc tccaccatct
      181 gatacattat acaaaatact aaatgtacta tatacagaac aagaggcgaa gttagttgct
      241 cagcttccaa taaagccttt cagagtaaaa acagcagcta aaatttggag tgtaagtgaa
      301 agtgaagcat atagagtatt ggataaactt gctagtaagg ctctgatact agatatagaa
      361 gataataaag ggaagaaata tataatgcct cctccaatgg ctggtttttt tgagtttgcc
      421 atgatgagaa cgagacacga tatagaccaa aaacttttgg cagagcttta ctatcaatat
      481 atgaatgtag aagaagattt tatcaaagat ttattttatt caacagaaac gaagcttgga
      541 agagtatacg ttcaagagga agttttaact aatgataatg aggttagtat tttagattat
      601 gaaagggcta ctcatataat tgatgaatca actcatatag gtataagtat gtgttactgt
      661 agacatagaa tgcaacatgt tggaaaagct tgtgatgctc ctatggatat atgtatgacg
      721 tttgacaatg tagcaaattc tttaataaat aataaatttg caagacgtgt ggataaaata
      781 gaatgtaaag agctacttca tcaagcatat gaacataact tagttcaatg tggagaaaat
      841 gttaggaaag gggttacgtt tatatgtaat tgctgtggat gttgctgtga agcaatggta
      901 gcagctaaaa gatttggaaa tcttcatcca gtacagacaa caagttttat accaaatata
      961 aaccatgaaa attgtgtaaa gtgtggtaaa tgtataactg catgtccaat agatgcgata
     1021 agcaaggtta aggaagatgg taaagaatat ataaaaattg atgaggatag atgtttaggt
     1081 tgtggagttt gtgttagaaa ttgccataaa aatagcatta tgttattaaa gagagatgaa
     1141 aaaataataa ctccagcaaa ttcagtacat agagcagttc ttatggcaat tgaaaaaggg
     1201 cagttacaaa atttaatttt tgataataat gctctggcta gtcatagagc tatgggagct
     1261 atactgtcag ctatattaaa gcttgaacca gctaaaaaga ttttagccag taaacaatta
     1321 aagtcagtat atttagataa attgttaagt atgaatgata aatagaaact aacttaaaaa
     1381 aggcaaagtt ttagacttat ctatatctta ataaacttat catttaatat ttaaatttaa
     1441 tttttacaca tataaaactt ataacttaaa taaaaaaagg ctatattcaa ttaatattac
     1501 ttgaatacag ccttttttag ttatgttaag ctatttcttt tatttgctta aatgagtttt
     1561 catttccatt tccaccaatt tgtgttacag atttaaatct catagtttta aatagttctt
     1621 tttgattata atctagtgag tttcctacta gcattacagg agatttagtt tttccagcca
     1681 aaactccaac agataatccg tctattaaat catcttgttt attcattcca tttttagcaa
     1741 catataaatt gtctatctta gaatccttgt aaaattcttt tattactttt gcattagttt
     1801 catttctagt gctacctgat attctctgtg gattttgaag tttactttct atatttttag
     1861 aaactgtata ttctccaccg attacataag atttatcaat ctttttgttt ttaaacaagt
     1921 tatttatttc agttatatta ctattttcat tagtaagtat tataggcata tcattttgag
     1981 ctgatacagc tccaatgctt actgcatcgg ctaatccttt ttcgccatta actactgaaa
     2041 tctttgatat attgctaata ttgttcaatt ctttagcaat gttcatagaa gtttgatatc
     2101 tatcacttcc tgaaattcta gatacagaga tattgtttga ttttatagta tctaaagatt
     2161 tttcatttat agaagcttct ccaccaacta catatacatt tttaactttt aatctcttta
     2221 attcagccaa agttttttca tttatttgag agcttccagt caataatata ggagcgtttt
     2281 tcttgtatgc aaatggtgtt gcagccaaag catctgcaat tgctgaatcg tttattaaaa
     2341 ctgcattttc agcattttcc caacctgctt gacttatctt aatagaagtt tcatatctat
     2401 cagttcctgt aagtgcactt gcatttactt catcttgata tgtaacctca aatctagcat
     2461 ttggacttcc taagaaaaca attacgcctc cttttggcaa tctaactcta ttgttctttg
     2521 taactaaaga atagtttaca ggaacacctt tatcatcata aacagcaaaa gctgaatttt
     2581 gaggaagatt aacttctatt tttttatttg cgatatcgtc accaatctta taccattttg
     2641 cataaccatt ggattctagt tcacaagtaa aagatttttc tgctggcaaa ttagttatag
     2701 aatcttcact aacatacgtt agtgttccaa aagataaata ctctgtacca ttttctttat
     2761 gaagtttaat gtcggataaa tcacgaccta ttacaccagg tatttctata aaagcatttg
     2821 agttattctc atccattatt ttagtgttaa ctatataacc tggagtttca tcagaaagag
     2881 caagagttgc cttcacagaa ccaaacatat agctttgaga agtatatttt tcatctacta
     2941 gatagtaatc tttaccattt ctttttttcc atgcttcttt tacactattt gatatattgt
     3001 tgtcatctat cttttgagct acatagtata aacttgctgt ctgacctaat ccagggacat
     3061 catcataaga gctcatattt aaatatgtga tattgttttt ttcttttaca aattttagac
     3121 agctgttacc tttttcagat acaaatctat cttgacctat gtatacgtat ttatcttcag
     3181 gtccattttc tatataagga gaagatactg ttaaagtacc attatcatta acatctactt
     3241 ttatcatatt tgaagaagca taaagaccac tattttcttt tagagagcta ggcattttaa
     3301 cttgttgtgg tttactaaat gttttatcag gttttatttc tttaatttta cctttttctt
     3361 ttaatgcaga tagtaagatt tcttgtccaa ttatttcatt aagttgacta cttccaccag
     3421 aagaaagaac tgcaactgcc atattttcat ctggaagaac tataagatta ctgtggaata
     3481 ataaagagtc tccacctttt gtaagagctt ttaaattata ctgattaaat ggataagtgt
     3541 tgacacaatc ccaccctaaa ccatatccaa gtatagagtc ttctccttca ggccatagac
     3601 catttaaata ttctttgttt tccatagctt ttactgaagc aggagaaaga ataccatttg
     3661 aatttttcat aaatgtttgt gcaaatgtac atagattttc tgcacttgaa tataaaccac
     3721 ctgcacctat agcattaagg ttatcttgcg gaactgcatc ttcccaataa ggcacgtatg
     3781 cttttgctag ttttgaacta tcaaaactat tttctgtagt ttttgtattt tgtaagttaa
     3841 gtggattatt tatgtattta tcaagaaaat tagtaaatga cataccagat actctttcta
     3901 ctaaaatttc agctaaagta aatccatcat tacaataaac ggaaaaagct cctggtttag
     3961 cttttaatct ttgtttttgt aattctttta agaaattatc atgaccataa gaatcattgt
     4021 ctgctaataa aatagtgttt ttaaaactac ttcccatcaa ccctgatgaa tgatttaata
     4081 acattcttgg agtgatttcc ttgtatctat catcagccat tttaaattca ggtatatact
     4141 ttacaacagg agtatctaaa tttaattttc catcatcaac caacttcata acagctgttg
     4201 tagtaaacat tttactgata gatgcgatac tatacatatt atccttattt aagtttttgt
     4261 tatcctgttt gctgtaaact ccaccattac cagaaatctc aattttgcca ttatcaatca
     4321 aagcatattg agcacttaca gcaccatact ttgatagcaa agtttggatt ttctctttag
     4381 caagagcttt agtttttgtg taagattccg cgtttttact ataatcatgt aattctatat
     4441 cagaactttg atatttttcg gaattatctt ttgtattatc agcaaaagta atatttggag
     4501 cagcagtaaa taacatactc aatactaatg tactagataa taatttagag agcctttgct
     4561 taaacatatt acattacctc tttccctttt taataattta taattagcat aacattctta
     4621 attattttaa tcaacagatt attatcaata aaaaaagctt ttataagtat agctttttat
     4681 ataacagaga aaaatataaa aataatattt gttgttgtat aaagaataaa gggtataggt
     4741 aatatataaa taaaatggag gaattttaga caatgtataa gaatataaat aaaaagcaac
     4801 ttaaagaaat gatgaaaaat gaaaaggatg ttttattatt agatgtaaga actaaagatg
     4861 aatttaagga atataaaata gaaaatgcta taaatatatc tttacaagag ttaatcaata
     4921 atatagatga aatttacgat tataaagata agaaagtagt agtttattgc agaagtggtc
     4981 atagaagtgt tactgcatgt aatttgttag ctgaagaagg ttttgagcat ttatataatt
     5041 taaattcagg aataattgat tatatgagtt aaataatgta agttatacat taaatactat
     5101 gtatagctct gcttattaag taggaatata tctaaatatt tataagataa atttaggaaa
     5161 gaacgaattt tttcgttttt ttctatgaca gtttttgtag taaatattag tttttaagtt
     5221 ggaaaaaggt agtagctttt tagctactac cttttaatat gaacttagaa tattattaaa
     5281 aaaaatatat ttaaagacag aattaatttt ttaatgacca gttaaattac tatcgccgaa
     5341 tttatcatta aatggtttta taactctatt tataggtcct tttagaagta catttaaaca
     5401 aatagacaga atcataaata gtaatagaac tagtatatct ttagttagat ttggagcata
     5461 tataccacca actgcttccc ttgtagcacc tattgcatag gtaaatggca ttatagggtt
     5521 tattctttgg aagaatgtag gtgtaacctg tatagggaat gtaccaccag aagaaccaat
     5581 ctgaacgact aatagtacta ttgcaattgc ttttcctaca tttccaaata cagaaactaa
     5641 agaatacact ataaagttaa ataccatact agtaaacata agcagaacta tcaataaaat
     5701 tggatgtttt gctgttactc ctaacaataa tatatctcca gttcctatta taaaaccttg
     5761 gacaagagct atacttaaaa aagtaaggcc tcttccaaag taaatttcat aaggtttata
     5821 gtttccatgt acagatgtag acaatagtgc tgaaataagt aatataccaa cccatgtaga
     5881 aagaacacta taaaatggtg tcattgcaga accataattt tcaattggat ataatttctc
     5941 tgttttcacc tcaacaggtt cttttaagaa attagacttt gtcaatatat cactttcaag
     6001 caagctgact aattttttca tatcttcacc attactaatt ttgcttaaag tatcaatcaa
     6061 atcatccaac attccttttg ctaaaggaag tctttctctt atcaatgata tactttcctg
     6121 tgcatttcct gaaagtttta gagaagtagt aagtatttct tcaacctttg gaagttttgc
     6181 ttctgcctta tctaaaacag taattatatc atttgctact tttatactgt ttgcaaatat
     6241 actatttata ggttttgata tttttgagtc aaaattattt aaaatattta aattaattct
     6301 tccaatacca tttgatagac ttaaaacatt attcaaagca cttatagatg gttgttgtcc
     6361 agatataact ttgtttttaa tatcatttaa tgttgatata gatgaatcta ttttattgct
     6421 agagtcttca agattatcta ttacatcatc caatctatta tttgatgtaa gttggtttaa
     6481 tttagttaaa aagtccacca aagtatcgtt taaagattgc aaattagaaa gtttttctga
     6541 taaattatct atcaacttag gtacatcttc actgccacta tttactgcat caattaaatt
     6601 taatgtaaga gaagaagcag aagatgaaag gtcaaccatt aaatttaagt cagacttaat
     6661 aagaggagaa agttcgtcta aattatcatt tgtatcctct aagaattttt ttaagtcact
     6721 tgataatagt tttgtatcat tcagagtctt tttaattgta ggtaaatcag atttaatatc
     6781 tttaactgta tcagataatt ttgaagtagc atcagaagct aaatcaacag tcttatttat
     6841 atggtcaaat ctcttttgaa ggtcaataag ggaattttca acttttgtca attttggaag
     6901 actgtgttca attcctgcac caactccgtt tgatatacca agaacgattt cactaactgt
     6961 ttttactata gtctgattaa cctgtagttg tattgtagaa gcacctttat ctgtaatctt
     7021 tggtgcaact gcatttattt tttcattaac agtatagata atttcgcctt ttttaacatc
     7081 atctgtaatt aaagatgtca aatcttttgt aaaatttttt ggaatcttaa gtgatgcata
     7141 ataagtacct tttttaacac cttcaagagc agttttttca tctacaaact gccaacctaa
     7201 actcttgtta tctttgagtt gttcaattaa ttggtctcct acattaatct tttgtcctgc
     7261 tatttcattt cctttatctt catttgttac tgctacaagc atattagcag tgcttccata
     7321 agggtcccaa gatgccttta tattgaacca agcatacaga gatggtaaaa aagcaagacc
     7381 taaaactatt acaagtacgg cataattgcc acgtatatct tttaggtcac ttttaaaaat
     7441 ttctaatata ttttttaaac cctttgaagg tcttaaattc ttgatattat tccagtttaa
     7501 aatttctttt aaactattag tttttttcaa atgtctcacc tcctaaaata ttttgataaa
     7561 gaaaaaagtt agatttataa tcaattttaa attatattac tttttacaca aatagtaaag
     7621 ttaaaatt
//
LOCUS       DAYAOX010000175         7601 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.176, whole genome shotgun sequence.
ACCESSION   DAYAOX010000175 DAYAOX010000000
VERSION     DAYAOX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.176"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            293..463
                     /locus_tag="ACGI57_002893"
     CDS             293..463
                     /locus_tag="ACGI57_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717957.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            1006..1884
                     /locus_tag="ACGI57_002894"
     CDS             1006..1884
                     /locus_tag="ACGI57_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFG9717958.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            1920..3815
                     /locus_tag="ACGI57_002895"
     CDS             1920..3815
                     /locus_tag="ACGI57_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFG9717959.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            4083..5990
                     /locus_tag="ACGI57_002896"
     CDS             4083..5990
                     /locus_tag="ACGI57_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9717960.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            5980..6429
                     /locus_tag="ACGI57_002897"
     CDS             5980..6429
                     /locus_tag="ACGI57_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9717961.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            6529..7416
                     /locus_tag="ACGI57_002898"
     CDS             6529..7416
                     /locus_tag="ACGI57_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFG9717962.1"
                     /translation="MAIYLEMPKLIHEFPFRTLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
BASE COUNT         3128 a          767 c         1313 g         2393 t
ORIGIN      
        1 ttataattgt tttaagataa aatgtaataa aatttaatgg agaaatttta taggtagata
       61 aattttaata tttataagat tatgttaagg aaacacttaa aaattagatt aaaattgtgg
      121 gtatttaaat caactaaaag atattgacta tttagtcaat atcttttttt attaaaaagg
      181 aatatacata taaataggtt agattaaaaa ttgggataaa tatgatttat atagcatcat
      241 ttaattaaaa ggagctatat tagtattcat tcatacaatt ggataatata caatgtcagc
      301 cttgctttat ggtgtttgta ataaatctat ttcagtttct attgtaatta atatgcaaga
      361 atttaagata ggagtatcta tggcttatgg tgtattagta attggtatat gctatatagc
      421 attaggaata atttttaaat tagatagaaa aagatttaca taagataaca aaggctaatt
      481 tataaattgg tgcttagaaa accaaatcac tttattagct agagatgaat ttggagggtg
      541 tcactttggt gttttatgta aagtttatgg aagtaaaaca ttagataatc gtgagtaaga
      601 tttctataaa tcgtatatac tattggaatc caagaaaagg tattggccaa atagctaata
      661 tctttttaat ttttttgact ctaatatagc attaactatt gcatgagtat ctttaaattt
      721 atagttaatg tatcttaatt gtttattata aaatctagga aggctaaagc ataaaacact
      781 taaaaagaga agatgtacat gagaaatata attaactatg atgcagactt ctaaaaatta
      841 tttagtgaaa aggaacttag agtgtataat aaaagttcct tttcactaag gaaaacgatt
      901 gttattttct gacattcatt accctgatat tatttaaata taaacaacaa caaaattaac
      961 aagaagcaaa ttcaacctaa aatttaaaat atcgggaggt acaatatgtt aataaacatg
     1021 aaggaaatgc taaaagtggc acaagaaaat caatttgctg taccagcatt taatatagga
     1081 agtgggcaaa tacttaaagc agtagtacag agtgctaacg aaaaaaatgc accagtaata
     1141 cttgcaatac acccaaatga gctaagtttt ctaggagata gctttgtagc tagttgcata
     1201 gaagaagcaa ataagagtaa agtacctatg gtaatacact tagatcatgg ggaaaataaa
     1261 gaacaaatat taagggcaat aagatgtgga tttacatcag taatgattga tggctcacat
     1321 ttaccatatg aagagaatgt agctatatct agggaaatag tagaaatagc aaaaggatta
     1381 aatgtgtccg tagaaggaga acttggaaca ataggaacaa caggaacatc aagtgaaggt
     1441 ggtactgatg aaataatata tacagattca aaattagcta aggattttgt tgaaaaaaca
     1501 ggagtagaca cactagctat tgcaattggt acagcacatg gtatatatcc aaaaggtttt
     1561 aaaccagagc ttaaactaga tttattaaaa gaaataagag aagtagtaga tataccttta
     1621 gttcttcatg gtggttcatc aaatccagat gaagaaatag cacaagcagt taaactagga
     1681 gtttgtaaaa taaatatttc gagtgatgtt aagtcggcat attataagaa gtgcagagaa
     1741 ctattagaac aaaacccaag cttatatgag ccagatacta tatatccacc atgtataaaa
     1801 gctgcaagag aagttataga gtttaaaatg aatctattca atgctataga taagttaaag
     1861 tgcttttata aaaataaaaa ttaattatga ggtaacaaat taaaaggagg ggagaattta
     1921 tgataggtaa aatactacga agcgattgta ttgtactaga taaacaatta caaacaaaaa
     1981 aagatgtgat taattatatg gcaaatcatt tgtaccatgg aaattatata aataataaag
     2041 aaaagtttat agctgatatt aataaacgtg aaaaagaatc atcaactaat atgggtaatt
     2101 taatagctat acctcatgct aagtcaaatg tagtaaatca acctgtaatg atgtatttaa
     2161 gaacagatag agagattaca gataatgatg gtcaaaaact taggtcattt tttatgatag
     2221 cagttccaga atcaggttca gatgaacatc ttaagattat atctaaacta gctacatatc
     2281 taatggaaga aaattttctt aagcatctac taagtgcaga aactccatac gaaatattaa
     2341 gtatatttaa taataaagct gcagaatcag gagattttag taataatcaa aataaaggtc
     2401 ttatagttgg gataacagga tgtccaacag gtatagctca tacttttatg gcaaaaaaag
     2461 cattagagga agcagggcaa gaacttggat atgaagttaa aatagaaact catggagcta
     2521 taggtgtaga aaataagttg actaaagaag atataaaaag agctgaatac gtaataatag
     2581 cttgtgataa agaagttcaa ctgagtagat ttataggaaa gaaagtgcta caaaagaaag
     2641 tagtagatgc taagaatgtt tcattagcta aaaatttaat tataaacgct gaaaatggaa
     2701 tggctaaaga aattaaacca ggtggaaaga ctttagaaga ttcaaaagaa atgcttcaag
     2761 ggtcagagaa aaagggtgtt tataagcact taatgagtgg tgtatctgaa atgattccat
     2821 ttgtagtgat aggtggaatt ggtatagcca tagcttttat gtttggtata tttgcatcag
     2881 acccaactca tgaaacatac aaccctatag ctggattctt tggacaactt ggaggcgatt
     2941 cagcatttaa gttatttata ccgatacttg ctggttttat agctaaatca atagcaggaa
     3001 gacaaggatt tgctcctgct atgatagctg gttttatggc agttattgga ggttcaggtt
     3061 ttataggtgg tatggtagct ggttttgcaa caggatatat agctttagga gtaatgaaga
     3121 taacagaaaa tttaccagat gtattaaaag gagtaaatgc tgtattgata tgtccacttt
     3181 tgattgcatt tacgagtgga gctataatgt tctttgtagt taatacgcca gcatcatggt
     3241 taaatgaagc tttacaatct tggttacaaa gtatgtctgg aactaataaa gtattattag
     3301 gtgcaatatt agcagggatg atggcatcag atatgggagg acctattaat aaaacagctt
     3361 cagcttttgg attggctatg ttttcaagta atatatttga accatcagca gcattaatgg
     3421 taggaggaat ggtacctcca ttagcaatat cattagctat gttaatattc aaaaataagt
     3481 ttactaaaga agaaagaaag tcatctctac caactgctat aatgggggca tctttcataa
     3541 cagaaggcgc aataccgttt gcagctgcag acccattaag aatgattcca gcaaatataa
     3601 ttggttcttg tataggtggg gcaatatgta tgagtttaga tataacttta atggcaccac
     3661 atgggggaat atttgtaata ccatttgcat gtagtgcacc agttgcatat atagcttgta
     3721 tattgattgg tacagtaata acagcattga ttataggggt aacaaagcct actataagga
     3781 gtgaaaatag tgaagttgaa ttacttggtg cttaaattat aaataatagt aaaaataatt
     3841 aatggaataa caacaagtat atagaataat aataaggata tttagaatca gttaactatt
     3901 tctaaatatc cttaattttt attttgaaat tatattatct attatatcaa tttagtaagg
     3961 tgtatcataa attcttttat gatacagaag tatcttagta attttacata gttagtaaat
     4021 atgttaaaat agtacttaat ataaacatag aaatatataa acataggtag ggagggctaa
     4081 gtatgcttgt gtcgaggcaa attgaatttc ttaaattgat actaaacgaa aataaatata
     4141 gacctataaa atactttaaa gataaattaa atgtatctga taaaacttta caaaaagatt
     4201 tgaagatgat tgagaaatat ttagaaactt ttaatataaa aattgatata aaaagaggct
     4261 atggaatatt aatagaagat ttagcaaaga agaacataga cttaattaat agtttaaata
     4321 tacaatcgaa ggaaaataaa tctttatcag tagagcagag aagaatggag attttaaagt
     4381 atttattgct aaattcaaat aatataactt caataaatca gttagcagat aaatattatg
     4441 taagtaaaac ttctatagta aatgatttta aatatataga ggcatggata aaagaataca
     4501 atttaaaact taataaaact ttagaaggaa ctaagattat aggtaaagaa gtggatataa
     4561 ggaaatcgat tgccaaaatg atggatgatt tattagatga aaattatgaa caacatgata
     4621 tatctgagtt aacaagatta gattctgcta catttagtgc attgataaat ttatttgata
     4681 ttgattctat tatatttgta gaaactataa ttacagaatt agaatacaat ttaggatata
     4741 ctataaatca gccctactat ataaatttaa ttacccatat acttatatgt ttaaagagaa
     4801 tcgaagaagg taatcaaata gaatctcagg aagaaagaga aatttctgta gataatttgg
     4861 acgagttagt atataaaaat gtcactacac ttatagataa gatagaaaat agatacagag
     4921 taaaaataac gaaagatgaa attatgtaca tatatatttt tttggtatca tctggattta
     4981 gtagcgaaaa taatagtagt tacaatgatg aatcagatag attaagtgaa agtgaaaaag
     5041 tttcatctat aatgatagag aatatgtcac gatttttaaa tataaatctc aaaaatgatg
     5101 acttactaca gaaaagtcta gcatctcatg taaggcctat gttaaacaga ttaagatatg
     5161 atatccaaat aaaaaacccg ttgttaggag agatacaaga gcgattttca gatgtgctgg
     5221 gattatgtat gatgactata aatattatga cagagtatga taatctgaag aatattagta
     5281 tagatgaagt ttcgtatctt gccacatatt ttcaagcggc aatagaaaga aatatgagta
     5341 gtaaaagagt aatagtagtt tgtcatacag gatatggaac atctcagctc cttgcagcca
     5401 gattaaaaag agaatttcct gaatggacta tagttgatat agttcctatg catcagttga
     5461 gtaagcgaaa tttagatgat gtagactttg taatatctac tgtaaactta gatttgaaag
     5521 ataaattaca tatagttgta tcagtactat taatggaaag tgatattaat aatataaaaa
     5581 atgctttact ttctaaacct aaaaaaagta tgaacttaga tacaaactta ttaggaatgt
     5641 atttagaaga taatatatat tttaattttg atgtagctca gatggaaagt ttaataggtg
     5701 tgaatttagg gagtaaatat gaatctattt cattaggaaa agatttaaat atatatatac
     5761 ataagcaaag taaattaaat aaagggataa tgaatatcaa taatgttact agaacgattg
     5821 atatttattt agaaattagt aatcaaagtt ttttaaagaa tattttaatg gaaatatcaa
     5881 atttatatag gcaaaagaac tatataaact atataataga ttgcaagagc aaagaggata
     5941 ttaaaatatt atttaagaat aatctattgg agaaaacaaa tggaaattag agaaatatta
     6001 acagaagaaa gcattatttt agatttgaaa tcaactacaa aagaaggcat aatacaggaa
     6061 ttagtagata aattatacga tacaggaagt ataactttaa aaagtaagtt tataaaggca
     6121 ttaaaacata gagaaaaaat aggctctact agtataggtt atcaaatggc catgccacat
     6181 ggaaaaacaa aatatgtaaa gaaggcatcc atagtaatag gtaaatctaa aaaaggtgta
     6241 gattggccaa gtatagatgg cttacctgta aaggtaataa ttatgtttgc tatgccacaa
     6301 tctggaggta atgaagatat gatattaata tctaaaatag cttacatttt aggtggtaaa
     6361 aatataggtg aaagactttt aaatgcacat acaaaaaaag aaatattaga tatattttat
     6421 gaagtgtagg tattgtataa attataaatt tgaggttttt aaaataaaat gtgtattctt
     6481 ttaaatagta tagattaatt tttagacact agagaggaga ggatatatat ggcaatatat
     6541 ttagaaatgc ctaaattaat acatgaattt ccttttcgta ctctatcaaa tgaaggagaa
     6601 gtattaacaa caccacactg gcataaggaa atagaaatat tacttataac agaaggtgta
     6661 gtaaatctat ttataaatga caagcctatg caattaaaaa aaggcgagat agtgataata
     6721 aaaggaggag atattcatta tatactgcca tcaccgggaa gtgaaagatt agtatttcaa
     6781 tttgatatat ctttttttaa tgatttaatt ttattaaatg aggagaaaga aagcttaatt
     6841 aatttattca gtagtataaa aaaatgtagt agagattggt cagcaaaaat aagaaagtca
     6901 gttttcgata tattgataga tatctacaat gaagacctat acaaaataga aggatatagt
     6961 tatgcaataa aaggtaaaat gtataattta attcctatat tatatagaca gattcctaga
     7021 gaaattgaag aaaatacagt agaatatgaa ataaactcta aagagatatt agagagattg
     7081 aacaatatat tcaaatatgt agaaaaaaat tataaacaac ctataaaatt agaagatgtg
     7141 tcttatgaag taggatttag tgtatattat tttactaaat ttttcaaaaa aaatacagga
     7201 aaaacattta taactttttt aaatgaatat agattagaaa aagcaaagtg gatattactt
     7261 aataacaata tatccattgt agatttggtt gaagaagttg gttttggaag tacaaaaact
     7321 ttttacagag tatttaaaga aaaaattgga gtctctccat tagaatttaa aaaaagatat
     7381 aatagcttgg agaaaaaagg gagtgtctca aaatgaactt agttcatgag gcactttctt
     7441 ttatatgaaa tcaaatatat tttcagtatt tcaacaatga aaattccatt catttataaa
     7501 gtctaaattt tagttctcaa tacaacctct atttcgaaat aaatcctttt atatatataa
     7561 atatacagca atatatgaga ataagataac aataatgagg a
//
LOCUS       DAYAOX010000176         7597 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.177, whole genome shotgun sequence.
ACCESSION   DAYAOX010000176 DAYAOX010000000
VERSION     DAYAOX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.177"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            186..1886
                     /locus_tag="ACGI57_002899"
     CDS             186..1886
                     /locus_tag="ACGI57_002899"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717963.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            2249..3436
                     /locus_tag="ACGI57_002900"
     CDS             2249..3436
                     /locus_tag="ACGI57_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717964.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            3496..4659
                     /locus_tag="ACGI57_002901"
     CDS             3496..4659
                     /locus_tag="ACGI57_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9717965.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGDNPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            4810..5613
                     /gene="zupT"
                     /locus_tag="ACGI57_002902"
     CDS             4810..5613
                     /gene="zupT"
                     /locus_tag="ACGI57_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFG9717966.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            5717..6268
                     /locus_tag="ACGI57_002903"
     CDS             5717..6268
                     /locus_tag="ACGI57_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HFG9717967.1"
                     /translation="MEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGIFYF
                     HKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYKDIP
                     PKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVLQII
                     LLILYAVQLYRRLMYYLRLKKFK"
     gene            6518..7231
                     /gene="rgbR"
                     /locus_tag="ACGI57_002904"
     CDS             6518..7231
                     /gene="rgbR"
                     /locus_tag="ACGI57_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HFG9717968.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT         2872 a          773 c         1376 g         2576 t
ORIGIN      
        1 ttaaatatag agatatagct ctttttatgt tgtttcaagt atacaactat atgatataaa
       61 tcttcaattt tatgaaaaag attgttatta ctttgtaagt ttataaaaga tttaaagtta
      121 ttgttatgtt atactaattt atgggtattt tttagaagta ctaataaagg agggatttaa
      181 tacttatgat aaaacaattt gtcaaatatt ataagccata taagaaaatt tttacactag
      241 accttatagc agcttttcta ttttccttat gtgatttagt ttatcctatg ataactagaa
      301 atataatgga tgacgttgtt ccaaataaaa acttaagaat gttagtagtt tttgcagtag
      361 cacttatact tatatttata gcaaaagcag ggcttaacta ttttatgcag tattggggac
      421 atgttattgg tgtagatatg caagctgata tgagaaatga ggtatttaca cacttacaaa
      481 gacttccaaa tacgtatttt gataataaca aatctggagt aactatgtct agaatagtaa
      541 atgatttaat ggatataaca gaacttgcac atcatgggcc tgaagattta ttcatttcta
      601 tagtaatgtt ggttggttca ttctttatac ttatagatat aaatattcct cttacgctta
      661 taatatttgc tatattaccg tttataattt ggtttgcaat agctaaaaaa gataagatga
      721 atattgcttt tatgaagagt agagttacta taggtgatgt aaatgctact ttagaaaata
      781 gtatagcagg tatgaaggta actaagtctt tttgtactga gaaagaagag ttaaataagt
      841 ttgtaagaag caataaatta tttagaagag caagacaaga ttcttataag gtaatggcag
      901 aatattactc tggtatgaat ttatacatgg atatcttaga gtgggtagtt gtaatagctg
      961 gtggttattt tacttatata ggaaagatta ctttgggtga ttttgctgct tatatactat
     1021 atgtaaaaat gtttatacaa cctatgaaaa aattaataaa ctttacagag caatatcaaa
     1081 atggtatgac aggttttaaa agattcatag agataatgga acaagaccat caaaaagaag
     1141 ctaaaaatcc tattgaactt gaaaatgtaa aaggtgacat tgagatagaa aatatatctt
     1201 tcacatatga agataagact caagttttgg ataatttaag tttatctata aaagctggta
     1261 agactatagc attagttgga ccttctggag gaggaaagac tacattatgt aatctattgc
     1321 caagatttta tgaatttgat aagggtgata taaaaattga tggaaagagt ataaaagatg
     1381 ttagtttaaa atcattgaga aaaaatatag gtatagttca acaagatgta tttttattta
     1441 caggaactat aagagataat attctttgtg ggaaccctaa tgctacagat gaagaaatga
     1501 tagctgctgc taaaaaagct agaatacatg actttgtaga gactttgcca gatggatatg
     1561 atacttatat aggagaaaga ggcgtaaaat tatcaggtgg gcaaaaacaa agaatttcaa
     1621 tttcaagaat ctttttaaag aatcctccaa taataatatt ggatgaggct acatctgctt
     1681 tagacaatgt aacagaaaga gaaattcaag agtctttaga ggaattaagt aaagatagaa
     1741 caaatttagt tgttgcacat agattgacta ctataaaaaa tgctgatgaa atcatagtat
     1801 tgacggataa aggaattgaa gaaagaggta ctcatgaaga gctagtaaac aaaaatggag
     1861 tatatagtag gttacacaat aactagtata caaattttac accatagcta acatacacat
     1921 taaacttaca aacataggat atattaagat tcataaagac aattcatttt atatttataa
     1981 gatgttttat gaataaaatg tatcctatgt tttatttttt gcaaattaca tttaattctc
     2041 tttgtaagaa tagagttaaa tagccaatgg tcgggataac gtttttacaa aaagaataat
     2101 ttagaaaact aactttaatt tatggaattt atcgaatttt atgaaaaaaa tcgaattttg
     2161 acttgaaatg tttttcaaaa gtagtaaaat aatggataac tatacataca ttttataaaa
     2221 atatatttat tatgaggggg gattttgtat ggaggcaata acagcttttt gtctagtttt
     2281 agtatcactg acgataggag atatagtttc agcaaaaact aaagcttttg ttccgtcggt
     2341 ttttgtatca gcaattatat ttatagtagg attttggact tttttcccag aaaatattgt
     2401 ggacttagcc gctttaggaa caccattagc tcaattaggt atgcttttgt taataactca
     2461 tatgggaact atgatgagta ttaaggaatt agcaggtcaa tggaaaacaa tagtaatagc
     2521 cttggcagga atagtaggaa tatgtgtagg tgctttggca ttagggacag tagtatttgg
     2581 atgggataca gcagtaatag ctacacctcc tcttactgga gggctagttg catctataat
     2641 gatggcagat gctgctacag caaaaggtct tgcaagttta tcagtacttg caattttgat
     2701 gtatgttgca caaggatttg cgggttatcc tattacagca ttaatgctta aaaaagaagg
     2761 aaaaagactt ttatcagact ttagaagtgg aaaagtaact gtaaatacag atgaagaaaa
     2821 agttaaggat ttacctgaac aaaaaagcag atttaagata ataccagact taccagaaaa
     2881 atatgatacg acatatatga taatacttag attaggattt gtagcatggt tagcagtaca
     2941 atttacaaat cttacaaatg aagttgtatc aagatatgtt atatgtttaa tatttggtgt
     3001 aatagcatca gaacttggaa tagttgatag aaaaccactt aataaatctg gttcatttgg
     3061 atggttaatg acatcattga tggcttttat atttgcaggg ttagcaaaag ctacaccagc
     3121 aatgcttgtt gaaatggcag ttcctcttgt aggaatcata atatttggtg taataggtat
     3181 gggaattttc tctattatag taggtaagct tttaggtgaa agtaaagaaa tggctttttc
     3241 agtttcgctt acatcacttt atggttttcc accaaactat atattaactg aagaggcagt
     3301 aaaggctttg gctgaaacac cagaagaaaa agaattttta atggataaaa tgcttccaaa
     3361 gatgcttgtt ggaggattta ctacagttac aatagcttct gtagtaatag caggtatctt
     3421 tgtaaatcta atatagtatt taattgataa aaagtatttt aaataagaag aatgtgtact
     3481 acttggaggt atagaatgga tattaaagaa ataacaaaga gttataaaga ctatgtaatt
     3541 aagcttagaa gggaatttca tgaaaatcct gagaaaagta tggaagaagt tagaacatca
     3601 aagagagtta aagaagaact tgataaaata gggataccat atgtatcagc tggaggtaca
     3661 ggtgtaattg caactataaa aggagataat ccaggaaaga ctgtagcact taggggtgat
     3721 atggatgctc tacaagttgt tgaatgcact gatgtagaat acaaatctaa aaatgaagga
     3781 cttatgcatg cttgtggtca tgatggtcac acatcaatgc ttctaggggc agcaaaagtg
     3841 ttaaatgata ttaaggatag tatcaatgga actgtaaaat tattttttca gccaggagaa
     3901 gaagttggta aaggtgcaag agctatgatt caagatggtg ctatggaagg tgtagatagt
     3961 gtgtttggaa tacatctttg gactgatgta gaatctggga ccatatcggt agaagaagga
     4021 ccaagaatgg cttctgctga tttctttaaa attactgtaa aaggtagagg tggtcatggt
     4081 tcacttcctc atcaaggtgt tgatgcagtg cttgcaagtt cagcaatagt tatgaatcta
     4141 caatctatgg ttagtagaga agtaagtcct ctggaaccat tagttgtgag tgttggagtt
     4201 ttaaattctg gaactagatt taatgtaatt gcgagtgaag ctatcttaga aggtacaata
     4261 aggctattta atccagagct tagaaagcaa attcctggaa ttttagaacg tatagcaaaa
     4321 tctacagcag aagcatatag agcagatgct gaattagagt atgggtactt aacaccagca
     4381 gttataaatg acaaagaatg ttcaaagata gctacagatg cagcaattaa gctatttgga
     4441 gaagactgta taacactatt tgaaaaagta acaggagcag aagatttagc tgagtttatg
     4501 aatatagcac ctggtgcact agcatttgta ggagctagaa atgagtcaaa aggtgcttgt
     4561 tatcctcatc atcatggatg ctttaatata gatgaagatg ctctagaaat tggaactgct
     4621 ttatatgtgc aatatgcagt ggatttctta aataaataat tttaatatac tataaagaaa
     4681 aagcagagac taagtttata tctctgcttt tttattgtgt atatttttta taaatattga
     4741 ctatataaaa tatatatgat agcataaaga aaatgataat caatatcaat taaaaagaag
     4801 gtgatttatt tgaataatgt aatatttgca tttttgataa cattattggc tggtttatct
     4861 actggtatag gaagttgtat agcatttttt gctaaaaaaa caaatactaa gtttctttct
     4921 ataagcttag gattttctgc tggtgttatg atttatgttt ctatgataga aatttttcct
     4981 aaagcacaag atgctttgac aaaatctatg ggagagaaat taggaagttg gtcaactgta
     5041 atagcttttt ttataggtat ggctatcatt gctgttattg ataagctaat tccacaagaa
     5101 gaaaatccac atgaaataaa gaaaatggag aatatagaag aaaagaatat caaaaaaaat
     5161 aagtctcttt taagaacagg tatatttaca gcaatggcta tagcaattca caattttcct
     5221 gaaggattag ctacatttat atcagctctt gatgatgtta caatagctat tccgatagca
     5281 attgcaattg caatacacaa tataccagaa ggtatatctg tatctgtacc agtttattat
     5341 gccacaggag ataaaaagaa agctttttat tattcctttt tatctggtat gtctgagcct
     5401 ttaggagcga ttattggata tgtacttctt agaaattttt taaatgatat aactcttgga
     5461 attgtatttg caattgtagg aggaattatg gtattcatat cactagatga attattgcca
     5521 tcagctagag agtacgggga acatcattta tctatttatg gattgattgc aggaatgggt
     5581 gttatggcta ttagtttatt actatttaaa taacttttaa atatatgatt gtaacctaaa
     5641 attagtatca aaatgatata attatttaaa tagattttta agctatttaa aaatatcatt
     5701 ttggagaatg agtgctatgg aaaagaaaat aaaattcaaa tttgactggt atagttcacc
     5761 tgataagata gagaaatggt tagaagatat tgaagctaaa ggatataatt tatataagat
     5821 aaataaattt ggaggaatat tttattttca taagggaagt ccaagaaaaa taaaatacac
     5881 tgttgattac tactatgatg tagagaataa ttattttgat gaacataaat caaatggctg
     5941 gaatcttgta tttaggagta ttggtagttt tatgggtaca tctgcatggt atatatggtc
     6001 gaaagaatac aaagacatac ctcctaaatt atatagtgat tctgataaaa caaataaagt
     6061 aaagtcaatt ttatttatga atatgtttat attctttatg ttagtagtaa tatttatata
     6121 tttattgaat attgaaataa gaaatttata tagtggttat ggagcatctg taactgctat
     6181 tgtactacag attatattgt tgattttata tgcagtacaa ttatatagga gattaatgta
     6241 ttatttaagg cttaaaaagt ttaaataaat aaaagaaggt gtcctaaaat tattttttta
     6301 atattttaag acatcttctt ttgtcagtaa gtactgtttt taattatagt aatttctgtc
     6361 tttaaattga aatatcaaat aatagttgta tgggattgag tattgataaa aataatttaa
     6421 aaatgttatc ctaaagtatc agatatacaa aattatcttt aaatatatta taaatatttt
     6481 aattgtatta actagaaatt atgtaaggag gcattttatg attaagatag cagtttgtga
     6541 agatgaaaaa gaaacacaac ttttaataga agattacctt gagaatatat taaaagatat
     6601 aagtatagaa tatgagatac aaaagtatat atctggagaa gagttactgg aaagtaattt
     6661 aaaagatata gatatattac ttcttgatat aaaaatggaa aaattaaatg gaatggatac
     6721 tgcaagaaag attagagaag tagacaatga aatggaaata atatttgtaa cttcattaat
     6781 agattatgtg caagaaggtt atgaggtaag agcttatagg tatttactaa agccaataga
     6841 gttggaagaa ctaaaaaaac atgtgctaac ttgtatcaaa gatattgaaa taaataagga
     6901 aagtcatatt acaataaaaa ataagtctaa tacatataag atttatttaa atgaaataaa
     6961 atatatagaa gttcaaaaaa aagatatgct aatacacaca ataaacaaga attttgatat
     7021 aaaatatagt ttaggcaaaa tagaaaaaga attaaatcca tataaattta taagatgcca
     7081 taaaagtttt atagtaaatt taagatatgt tgaaaatata aagcctaata ctgcaatact
     7141 ggaaagtgga gaagaggtac ctattagtag atatagatat aaagaagtta aggagaaatt
     7201 cttaaaattt cttggtgata caatatgcta aactttatga ctgagctatt agtagtttta
     7261 tctatattta tacagttttc tataatgaac ggaattttaa tttataaaaa ggatagtaaa
     7321 aaaaatgaaa aaatagtatt ctatattgtc ttgtttctta tttatattat aattcagata
     7381 agtagattat ttaatggagg aaattacttt agtaatataa actatttatt ttatatatta
     7441 gcatttattg cttggggagg atactataat tatttttata agatgggtta tattaagtat
     7501 tccattttct tttatctatt tagtgtatat tataaatgtt tggatgctgt tatatataga
     7561 atactatttt ttgcaattac aggtaatgtt atttcat
//
LOCUS       DAYAOX010000177         7595 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.178, whole genome shotgun sequence.
ACCESSION   DAYAOX010000177 DAYAOX010000000
VERSION     DAYAOX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.178"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(83..1354)
                     /gene="hflX"
                     /locus_tag="ACGI57_002905"
     CDS             complement(83..1354)
                     /gene="hflX"
                     /locus_tag="ACGI57_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFG9717969.1"
                     /translation="MAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSITQN
                     AKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRTAL
                     ILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKLEL
                     DRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLVDI
                     FKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRSTL
                     EEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVLDR
                     ILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNENTS
                     IINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            complement(2079..3911)
                     /locus_tag="ACGI57_002906"
     CDS             complement(2079..3911)
                     /locus_tag="ACGI57_002906"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717970.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(3904..6150)
                     /locus_tag="ACGI57_002907"
     CDS             complement(3904..6150)
                     /locus_tag="ACGI57_002907"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9717971.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(6218..6679)
                     /locus_tag="ACGI57_002908"
     CDS             complement(6218..6679)
                     /locus_tag="ACGI57_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9717972.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(6984..7550)
                     /locus_tag="ACGI57_002909"
     CDS             complement(6984..7550)
                     /locus_tag="ACGI57_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFG9717973.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         2437 a         1388 c          785 g         2985 t
ORIGIN      
        1 gtttcataaa atcatattta caatcaattt ataataaatt ataaattaaa tatattttag
       61 gctataataa gtttttcaaa atttactcta aagcatactt tttatactta ttatattcta
      121 tattgctaca ttctacatct aaaatagctc catcttctct gtattctgta tttataatgc
      181 tcgtattttc gttaaaataa gaaactacat gacctttatc atatggaatt aagaacttac
      241 atcttacata atctttaaat atgctatcac aaatacattt tatcatttta tctatattta
      301 tatctttttt aactgaaaca aatatacctt ctttatcgat actatctaaa attctatcca
      361 atacttctac atctattaaa tcaattttat tatacacatg tatcataggt ataccatctg
      421 cacctatttg ttttaaagta tcttctgtca ctttaatatg acttttatag cttggatttg
      481 aaatatcaat aacatgtaac agtacgtctg cctcacatgc ttcttctaat gttgacctaa
      541 atgcttttac taagtcatgt ggtagattac ttacaaaacc aacagtatca gataataaaa
      601 attctttgtt atttgcaaga actatatttc tgacagaagt ttcaagtgta gcaaataaca
      661 tgtttttctc aaacactttc ttttcttcag agtttttaaa tatatctact agtttattca
      721 ttattgaaga tttaccagca tttgtatacc cgactagagc tacatttgga agatttgatt
      781 ttcttctcat acttctttga gtttctcttt ggaattttaa atcatccaac tccctattta
      841 aagatgttat cttttcttct attcttctac ggtcaagttc taattttttc tcaccagaac
      901 ctctattttt agtaccaact ccaccacttt gacgtcctaa attttcattt gaacctataa
      961 gtcttggtaa catatatttt aaacttgcta cttctacttg aagtttagct tctctagttt
     1021 ttgccctttg tgcaaatata tcaagtatca atgccgttct atctataatt tcacaattaa
     1081 gcttttcttc aagatttttt agttgtgatg tagaaagttc attgtaaaat ataactatct
     1141 ctatattttc tttctttatt aagtttaata tttcctcaac tttacctgtc ccaatgtaaa
     1201 atgctctatt aacctgcttt gcattttgag ttatactccc tacaacatca atatcacatg
     1261 ctgaacataa gttttcaagt tcaatcatta gttcattaaa gtcctcagat ttattattta
     1321 tgtttaatcc tactgttatt ccttttttcg ccatatccat attctcctta tctgctattt
     1381 tttatttaat ctatagcacg agattatggc tctataatta aattcatcaa gaattttgct
     1441 ttttatctta tccttaaatg acacttctta tttactatta taataaccat aatcgtgcat
     1501 tattttctca atcttatata aaaaatcact ttcttacacc tcctcccaaa tatattatgc
     1561 agttttttca ataaaaaaac tccttacaaa tctaagtaat acaaaacaag ctacttatat
     1621 cataagaagg attaaaattc taaatatata ttaaaaatac attagtttaa ttaacttata
     1681 tgtaattatc attttatatt taaccctatt cttaaattta agtagttatt tgcatgatta
     1741 ataaaagtaa taaaaacata tacttcaata taatcatcaa ctattaaaat taaattctat
     1801 ttataataaa atttaaagaa taaaattaaa tatacatttt gataatcacc tccaacattt
     1861 tccttactac atattataat aaaataaaca ctttgtaaag acataaaatt atgtggctat
     1921 ctcaaaatat agagaatcct ctttttcatt gttaaaatga taaaaataca ctttatttca
     1981 tacacaaaag agtgccttat gaattaaaaa gttcatttta aggcaccctc atttatttta
     2041 tcttattaat tacatgtcta tgctaagata gttttttgtt acattgcttc atcttcttca
     2101 aattgagaat tgtaaagatt ggcatagaat ccgcctttct ttagaagttc ctcgtgattt
     2161 ccttgttcaa ctatatctcc ttcattcatt acaagaatca tatcagcatc acgtatagtt
     2221 gataatctgt gggctataac aaaacttgtt ctaccttcca taagattatc catagctttt
     2281 tgaatcaata cttctgttct agtatcgaca gaacttgtag cttcatctaa tattaaaatt
     2341 ttaggatcag ctaaaatagc acgagctata gtcaataatt gtttttgacc ttgagaaaca
     2401 ttacttgctt cttcatttaa tttcatctta tatccatctg gtaatgtctt aataaatcta
     2461 tgaacatgtg cagatttagc agcctctata acttcttcat ctgtagcatt caacttacca
     2521 tatcttatat tttccataat tgaaccacta aataaccatg tatcttgaag aaccatacca
     2581 aacatttctc taagctcact acgattaaaa tctttaatat tatgaccatc aatcagtatt
     2641 gaaccgctat taacatcata aaatctcatt aacagtttaa ctattgtagt tttacctgct
     2701 cctgtaggtc caacaatagc aactttttga ccaggtttaa catttactga gaaatcattt
     2761 attatagtct tgtttgggtt atatccaaag ttgacatttt caaaatcaat tttaccttct
     2821 aaaccatcta ttgaaactgc attttcaaca atttggactt cttcttcctc ttctaaaaac
     2881 tcaaatactc tttcagaagc tgctgctgtt gattgcaata aattggcaac ctgtgcaatc
     2941 tgagtcattg gctgtgtaaa gtttcttaca tattgtataa atgactgtat gtcaccaacc
     3001 tcaattgttt tcttaattgc aagccaacct cctaagattg ataccattac atatcccaag
     3061 tttccaataa acatcataag tggttgcatt atacctgata aaaattgaga tttccacgct
     3121 gaattataca ggctatcatt tagtttatca aactcttcaa ttacttcatc ctcacgatta
     3181 aatgctttta ctattgtttg gccactgtaa gtttcctcaa cttgaccatt tacatttcct
     3241 aaatattctt gttgatatct aaagtatttt tgtgatttct ttattattct tgaaattaca
     3301 aacattgata ttggtaatat taaaactgcc actaatgtca taattccact tatagataac
     3361 atcattatca aaacacctat catagttgtt actgatgtta taagctgtgt catactttga
     3421 tttaaacttt gatttaaagt atctatatca ttcgtaattc tcgataatat ttcaccatgc
     3481 gtcttagtat caaaatactt cattggcaat ctatccaatt ttgctgaaat ctcttttctt
     3541 aaattatagg atactttttg agaaattcca ctcattataa atccttgtat aaaagagaac
     3601 agtgcactta tcaaataaag gaaaagtaaa aaggttaaga tttttccaat tgcattaaaa
     3661 tctataccta cgtttcctcc agaaacttta ctcactagcc cttcaaatat ctttgtagtt
     3721 gcctttccta aaatttttgg acctactata gaaaacgacg cactaccaat agcaaataca
     3781 attactagaa ttattgatat tttaaattta gataaatatg caattagttt tcgcatagtc
     3841 cctttaaagt catttgcttt ttgtcctgct cccatagatt ttcccatagg acctcttccc
     3901 attctactca ttagctagct cctcctttga aagttgtgat aaagctattt gctcataaac
     3961 ttcgcaattt tttagaagct ctttatgtgt tcctattcca actatttcgc cctcatttaa
     4021 aacaattatc tgttctgcat ttaagatagt actaattctt tgtgcaacta ttagaactgt
     4081 actttcagca gtttttgatt ttaaagcctt acgaagagct gcatcagttt ttaagtcaag
     4141 tgctgaaaaa ctatcatcaa atatataaac ttctggattt tttgctattg ctcttgcaat
     4201 agaaagtctt tgtttttgac ctccagatac atttgttcca ccttgagaaa tttcagtttt
     4261 aaaagcttcc ggtttagaat ctataaattc tgtagcttga gctatttcag ctgctgttct
     4321 catttcttca tctgttgcgt cttttctacc atattttaag ttggattcta tagtaccaga
     4381 aaacaatact cccttttgag gtacatatcc aattttttcc cttaaatcat gttgagaaac
     4441 attttttata ttaactccac caagtaatat ttctccttca gtaacatcaa agaaacgagg
     4501 aattaaattt ataagagttg acttaccact accagtactt cctataaagg cagttgtttc
     4561 tcctggcttt gctgtaaaag atatatttgt taatataggt tcttctgcgt ctggatacat
     4621 gaacgaaaca tttttaaatt caacaagacc ctttttatct tccttaaact cttgtgcctc
     4681 tgatttttct gggtctttta tgcttatatc tgacactaat acttcatcta tacgtgtagc
     4741 tgaaacggat gctcttggaa gcataataga taccattgat atcattaaga atgacattat
     4801 tatttgcatt gtatattgta taaatgccat catatctcca acttgcattg ctccagaatc
     4861 aacactgtat gcacctctcc aaactataag aactgtgata aggttcatta taagcatcat
     4921 agcaggcatc atacatgtca taactctatt tacaaaaaga tttgttttag ttaaattttt
     4981 atttgcttta tcaaatcttt cttcttcatg tttttcagta ctaaaagctc tgataactgg
     5041 aataccagta attatttcac ggcttactaa gtttaactta tcaacaagct tttgaaccag
     5101 tttaaactta ggcataacca ctgagaataa cactacaatt aatgataaga tagcaactac
     5161 tgctactgct ataatccagg ccattgaagt atttgtgttt aaaaccttta tcactccacc
     5221 tatagccatt ataggtgcat agaatactat tctaagcaac ataaccatca acatttgtat
     5281 ctgttgaata tcatttgtac tacgagtaat taatgaagct gtagaaaatt cattcatttc
     5341 agtatttgaa aatgacataa cttttctaaa tactccactt cttaaatttc tacctacact
     5401 tgcggctact cttgctgcta aaaatcctac tgttattgta gctaccatac taatcattgc
     5461 tataccaagc attttagcac cagtaaataa tatataatta ttttgagttt tttctgtatc
     5521 aatacctatt ttctcatatt ctgaccttac atatgaaaca gagctttgtg ttatcatact
     5581 ctctggcata ctttcaaagc ctttgctcat ctctttagtc aatgtatcta actgttcctt
     5641 tggcattgct gataacaatt taaatatatc aacatctcca ctttgagtag cttgtggtgg
     5701 aagtttgctc attaactgag ctttcatagc ttttacctct ttactatcgc cctcaagccc
     5761 tgatactatt aaaatagctt taccaaaaat aacatttaaa tcatctatag tgtctttgtc
     5821 tttagtattt aactcataaa gagattcatc ttttagacct ggatacttat ctttatactt
     5881 aacataatcc tttttattca gcaaagtata attatctagt actttatcct tactactttt
     5941 atccataaat aaagtaattt tatttaattc actctctcta atcacagatg gaacagagtt
     6001 ttctactcca ccttgttgta tacctacatt tactatattg gatgtgtatt ctggtaaaga
     6061 aaggtcacat accgcttgaa ttaccaatag acagactatt attactattg ataatgccga
     6121 tttctttaaa tatttaatta atttaaacat acatttcttc ccttctgtaa tattaattag
     6181 tctaataaca ttaaattgaa aaacattatt ttaaacttta aaaatatttt tcatcttcat
     6241 gcttatgaca tcctgctttt aaattatctc gtacttgcat tagaagtctt ctaagtacta
     6301 ttttttcttc ttcactaaat cctaaaaaaa actctctttc caattccttg ttaaattctt
     6361 caactttatt acatatttct aaacctttgt ctgtcaaaaa aatcctagat actctttggt
     6421 ccttttcatc ttgttttctt tccacaagtc cagctttttc catcctcttt atcataactg
     6481 tagttgtaga tgcttttact ttccttttat ctgctaattc tttttgactt tgcccatctt
     6541 tcatctttaa agaaaaaagt atttggggtt gaccatgata taagcctaat ttatcaaaat
     6601 aaaaatgagt tcttataaaa tatattcgca ttaattgcaa gaaaacatca tttaattttt
     6661 cttcagaatt gctattcata cttgccctcc ttatttttta tattacttag tcggctaagc
     6721 attattttac tatactgcat gcattttttc aatatattta taaaaaattc atataaattt
     6781 catttattaa atatttttat atcatatagt tataatttta ataaataata gtaagatttt
     6841 taattagtca gctacttata ttttttattg taaagcttaa aataaacctt ttcatatatt
     6901 ttcaaataaa aaagttgtcc caaaataaat tttgaaacaa catcatattc aacagtatct
     6961 tgattatata acattatgat actctactta tcttcatatc catttacaac aacgtctaac
     7021 ttcccagttt caggtgatat aattagacca tgtacaataa taccatctgg aattaaagga
     7081 tgccctttta ttaaatcaat actatctttt acagattctt ctgcagaatc gaatccatgt
     7141 aaccattgtt ttgtatcaat tccagcacta cgaagtgtaa gtattacatc attagatatt
     7201 cctctatcta aaattttacc aagtaactta tccgtatcaa cattacacat accacatcca
     7261 tgatgcccta caaccataac ctcatctaca tcaaattcgt aaattgcaac taaaacactt
     7321 ctcataatac ttccaaaagg atgcattata gtagctcctg cattttttat tagtttcaca
     7381 tctccatttt tcaaattcat agccttaggc aaaagctcag ttaatcttgt atccatacaa
     7441 gataagacta ctattttttt atttggatgt tttgatgtca catattgttc atattctttg
     7501 ttctctacaa atgatttatt atattctaat atctcttcaa gttttctcat atgtaatatc
     7561 accctcttta aaaatttttt tattattata tcact
//
LOCUS       DAYAOX010000178         7562 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.179, whole genome shotgun sequence.
ACCESSION   DAYAOX010000178 DAYAOX010000000
VERSION     DAYAOX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.179"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            19..720
                     /locus_tag="ACGI57_002910"
     CDS             19..720
                     /locus_tag="ACGI57_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9717974.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            944..2245
                     /locus_tag="ACGI57_002911"
     CDS             944..2245
                     /locus_tag="ACGI57_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9717975.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            2382..3476
                     /locus_tag="ACGI57_002912"
     CDS             2382..3476
                     /locus_tag="ACGI57_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFG9717976.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKLILEGE"
     gene            3478..4611
                     /locus_tag="ACGI57_002913"
     CDS             3478..4611
                     /locus_tag="ACGI57_002913"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFG9717977.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELRRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            4629..5738
                     /locus_tag="ACGI57_002914"
     CDS             4629..5738
                     /locus_tag="ACGI57_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFG9717978.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            5803..6543
                     /locus_tag="ACGI57_002915"
     CDS             5803..6543
                     /locus_tag="ACGI57_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFG9717979.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGILSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            6548..7540
                     /locus_tag="ACGI57_002916"
     CDS             6548..7540
                     /locus_tag="ACGI57_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFG9717980.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYITTGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
BASE COUNT         3013 a          785 c         1473 g         2291 t
ORIGIN      
        1 taatttaaga ggtgaatcat gttaagaaat caaccattat atatacaagt tgtagatata
       61 ttaaagcaaa aaatattaaa taacgaatat aaaattgatg aaaatattcc aacagaaagg
      121 gagttagagc aagagtttgg agtcagtaaa attactatta gaaaagctat tgagatatta
      181 gaatcagaag gatatgtaga gaaaaaaagt ggaaaaggga caaaagtaat aagtaatagc
      241 ctttttaata agctatcaaa agcagagtct ttttcaagta tacttttatc taaaggtcat
      301 aagttatcta aagagactat ttcaattgaa aaaataataa atgatacaaa ttcacaagta
      361 tttagtatat ttggagaaaa atgtagcaag attactagaa tgtattattt agatagtaag
      421 ccatatatat tttttactca ttatattcct tttgatgaaa atctaactgt tgaaagtttt
      481 aataatgaaa gttctttata tatgtactta tataggagaa atataaatat aactagattt
      541 gaagatgaat tttttataga agaatcagaa ctagaggtat caaaggcttt aaatctagct
      601 aaagacatat tattaggaag aaaaagaaag acctatgatg agaatgataa tatagttgag
      661 ctatcatttg caagatataa tacagaagta caaaactatg taattaaata cgaaatataa
      721 taagaatcct gagtttattg aaatgataat atgctcagga tttttgttta gttgtaaaaa
      781 taaaaaatat tataacatta taatgtttac aaccaaacat tataatgtta taatattttt
      841 aataaacaag tgatattatc aattacataa aaattagttt atgttatgaa atcgttattg
      901 cattataaat aaaggataat atcaataata cagggggata actatggaaa aattcgcaag
      961 ttttttagag cgcaagatta tgcctgtagc agcgaagata agtaatcaga gacatatgag
     1021 agctgtaaga gcaggtataa tagcaacatt accactaaca atagtggggt catttttcac
     1081 aatattatta aacataccta ttgatgctta tatggatttt atagctccat ttaaagcaat
     1141 attagatata ccatttagat atactgtagg atttttatct ctatatgcta cttttgggat
     1201 agcatcatca cttgctaaaa gctacgattt agatacaaca ggtgttggta tgcttgcagt
     1261 tatggcattt ttagtttcta caatagttcc aactcaagta gttgaaccag taggtggagt
     1321 tatagaagct ggtagatgga tggatatggc aaaacttaat tcgccatcat tatttggagc
     1381 tatcgtaaca tcattaatta cagttgaaat atatcgtttt atgaaggaaa agaatattac
     1441 aattaagatg ccagcaggag ttccagtaga ggtttcaaat tcatttactg cattattacc
     1501 gacagcagta attttaatgt tattctgggt aataagacat attctaggat ttgatataag
     1561 tgttgcacta agcgctatgt tatcaccttt aaaaggattc ttatcaggaa atagtttagg
     1621 tggtgggtta ctgacagtat tcctaataac tttattctgg gtacttggaa tacatggtcc
     1681 agctataatg ggaccagtaa taagacctat atgggacatg tctatagcag aaaatataac
     1741 tgcattcaca gaaggagtat cagctcatgc aatgccaaac atatttacag aacaattttt
     1801 acagtggtat gtatggatag ggggagcagg ggctactctt gcattagttg taatgtttat
     1861 gttttctaag tcacaatatc taaaaagttt aggtagatta ggatttttac cagggttatt
     1921 caacataaat gaacctataa tatttggagc accaatagtt atgaatccaa tactagggat
     1981 accatttata attggaccta taataatgac aattctatca tatgttctta ctattacagg
     2041 ggttatacct atgatggtag caagattacc atttgcaatg ccagcaccaa tagcagcagt
     2101 aatgagtact aactggagta tactagcagg tatcttagta ttaatcaact ttgtgatatc
     2161 atttgctgtt tattatccat tcttcaaagt ttttgaaaaa caacaattac aaagagaaca
     2221 agaagaaatg agtgaaaatt tataaaaata catagatata gaggttttgt actatataaa
     2281 atatagaaaa agcttataag ttattaaggg tgaaggtagg aggaatgacc tttgccttta
     2341 attttatata ttttaaataa tgtaatttga ggaggagaaa gatggaaaga agattaggaa
     2401 tatcagtata tccagagcat agtaacatgg aagatgataa ggcatatata aaaatggctt
     2461 caaaatacgg atttaaaaga atatttatgt gcatgctttc aattacaaga cctaaggaag
     2521 aagttaaaaa acattttaaa gagattataa cctatgcaag agaattagga tttgaagtaa
     2581 tccttgatat agctccaaac atatttgata tattacaaat atcttatgat gatttaacat
     2641 tttttagtga gttaaatgct agtggaataa ggcttgacta tggatatgat ggaagtaaag
     2701 aagctatgat ttctttcaat ccatatgatt taaaagtgga actaaatatg agtaatgatg
     2761 tagcatactt ggataatata attacttaca agccaaatgt aaataatata tatggttgtc
     2821 acaattttta tcctcaaagg ggtacaggct tgccatatga tttttttgta agatgttcaa
     2881 atagatttaa aaagtatgga ataagaactg ctgcttttgt taattcaaaa tacgcaacaa
     2941 tagggccttg gaatataaat gatgggctgt gtacgcttga aatgcataga aacttagata
     3001 ttgctacagc aaccaagcac ttattcgcaa caggattgat tgatgatgta attgtaggga
     3061 atgcatatgc aagtgaagat gaattaaaaa gaatgtctga agtaaataaa tatcaagttg
     3121 tatttaatgt agtaactgaa gaaaaaactt ctgaagtaga aaagacaata atggcaaatg
     3181 agcaacactt tagaagaggg gatataacca accaagtagc aaggtctaca gaagtaagaa
     3241 aaaaatatgc taaagaaaat ttccctaagc atgataatac gaccatcttt aaaagaggag
     3301 atgtagtgat tggtaatgat gactttggaa agtataaagg ggaattacag ttgatacttc
     3361 aagattgtgc agatgaaaga aaaaataagg tgggtagaat aaaggaagac gaaataatac
     3421 ttttagactt tattgaacct tggagtaaat ttaaacttat tttagaggga gagtaaagtg
     3481 aagatagaca tattaataaa atgtggaaaa acagtagata aaagtataat tgatatagct
     3541 atattagacg ataaaataat agaagtaaaa aatcagatag atgattctaa gtatcaggca
     3601 aatcaaatta taaacctaga tggagaaaaa tttattagtg ctggttggat agatatacat
     3661 acgcactgct acgaaagact agacctttat aaggattatc cagatgaggt tggaataaaa
     3721 agcggtgtta ctactgtagt agatgcagga acaacaggag ctttagatat aggtaaattt
     3781 tatgaagata ctaaaaaata taaaacaaat gtatatgctt taataaatat tgctaagcag
     3841 ggaataacat ctcaagatga actttcaagt atgatgaata ttgatgaata tgaacttaga
     3901 agagcagtta aaaaatacaa agattttgtt gttggaatca aagctagaat gagtaaaagt
     3961 gttgtaattt caaatgatgt tgaaccttta aaagtagcta aaagaataaa aaatgaactt
     4021 aatctaccta tgatggttca ctttgggagt tctcctccaa cgatagagga tatatttgac
     4081 tatatggaaa aaggagatat attaacccat atatataatg gaaagccaaa tggaatactt
     4141 agaggcaatg aggttaaaaa agaaatcata gaagctagag aaagaggtat aatccttgat
     4201 gtaggtcatg gaaccgaaag ttttagtatg gatattgcaa tgaaatctaa ggatgcagga
     4261 atttttccag acactataag tacagatata tatattaaaa atagaataaa tggtccagtg
     4321 tataatctat ctacaacaat ggaaaagttt atatatatgg gctattcact tgaagatata
     4381 atagataaag ttactaaaaa tgcagcagat gcaataagtt taaaaaataa aggtttaata
     4441 aaagaaggat atgatgctga tttaactata tttgatgtgg ttaatgaaga aaaagaactt
     4501 caagattctt tgaataagtc agtaatcaca agtacgtcta taaagccaag agcagtagta
     4561 gtcaatggag aatatttaaa tataggtaag tctataggag agaatgagta gaagggagaa
     4621 actattgtat gagtagtata tttgaaaaat actctgtaag acgtgttata aatgcttcag
     4681 gcaaaatgac tatactagga gtatcaacag tagataagga agttattgaa gcaataggtg
     4741 aagggtgtaa aaatttcttt ataatagaag aacttataaa taaaacaggt gaatatatag
     4801 caaatttatt agggtgtgaa agtgcactaa tagtttctag tgcatctgct gggattgcac
     4861 agagtgtggg agcaatgata gcaaaagatg atatgagtct agtttacaat gtaaataatc
     4921 ctaagaaaca agttaaaagg gaaataataa ttccaaaggg acataatgtt gattatggag
     4981 ttccagtaga agtaatgata agacttggag gaggagaagt tgttgaggcc ggttatgcta
     5041 atatgtgtaa caaggaacat ataataagag agataaatga aaatacagca gcaattatgt
     5101 atattaaatc tcatcattgt gttcaaaaga gtatgctaag tgtattagat gcagtacaag
     5161 ttgctaaaga atataattta cctttgatag tagatgcagc agcagaagaa gatttaaatg
     5221 tatattatga attgggagca gacctagtga tatacagtgg tgctaaggct atagagggtc
     5281 ctagctcagg gttagtaata ggtaagaaaa aatacataga taatgtaaag cttcaatcaa
     5341 agggtatagg aagagctatg aaaataggaa aagaaaatat tgtaggtcta acatgtgcaa
     5401 ttgagagata tataagtaat gataaagtaa ctttaaaaga aatggaggat aagctaaatc
     5461 catttataga caagataaat actataaagg gagtgagtgc tagtataact agggattcag
     5521 caggtagaga aatacttcgt ggagaaattg actttaatga agatataata aataagtcaa
     5581 cacaacaaat tataaatgaa cttagaagtg gagagatagc tatatacact agagactata
     5641 aggctaatga aggcaagatt gagattgata taagaagtgt aactggaagt gatttagata
     5701 ctatttataa taggataaaa aatatagtag aaagttagag tgtagagtaa gtgacattat
     5761 aatgatttaa taaattaaat tttaccaatg gaggggataa atatgaattg tataccacaa
     5821 tattataaaa atagagtttg tttaaatgtt cttgcaggaa gcataaaaaa tgctaaagaa
     5881 gtatatgatg cttgtgacgg gcatgtattg ataggaatac tatcaaagaa ttatagcact
     5941 gtagaagcag ctattgaaga tatgaaaaaa tactcaaagg aaatagataa tgcaatatct
     6001 gtggggcttg gagctggaga cccaaatcag tcaaatatgg tatctcagat ttctaaagaa
     6061 cttcagccac aacatataaa tcaagtgttt acaggtgtgg ctacgtctag agcattgctt
     6121 ggtcaaaatg atagtataat aaatggatta gtttctccaa ctggtaaacc aggatttgta
     6181 aaaatatcca caggtccatt aagttcaaaa gaaaaagatg gtatagttcc tatagaaaca
     6241 gcaatagcta tgatgaaaga tatgggagga agctcaatta agttttttcc aatgggaggg
     6301 cttaaaacaa aggatgaata tatagaagtt gcaaaagctt gtgcaaagca taatttttat
     6361 ttagaaccaa cagggggtat agacttagat aacttcaaag agatagttca aattgcactt
     6421 gatgctggtg tagaaaaagt tatacctcat gtttatacat caataattga taaagaaaca
     6481 ggtgaaacga aagtggaaga tataggagta ctttataaaa taatgaaaga attattggga
     6541 tagatttatg aatatattag cttttggtga aataatgatg agactttcag tacctgacta
     6601 caagttctta acacaaacca atgaactaaa ttatataact acaggcacgg gattaaatat
     6661 attatctgga cttaaaaact ttggatacaa tacctatatg ttgacaaagt tgcctaataa
     6721 taatgtaggt aaagcatcaa gtgcaaatat aagaaagctt ggagtaaaag atgactttat
     6781 cacgtatgga gggaatcata taggagtgta ttttcttgaa aatgggtatg gagaaagacc
     6841 ttctgaagtg acttatttga ataggttaaa tagttcattt tgtgagagta aattacaaga
     6901 ttatgatatt gataaatgtt tagaagaaat ggatatagtc catatttgtg gtattgctat
     6961 gcaacttaca gattcagtta aagagattgc tttagaacta gctagaaaat cttatgaaaa
     7021 ggggataaaa gtattctttg attttaattt tagacaatca ttaaatccag aaaggactta
     7081 tgatgattta gtaaatgatt ataagaaaat actaccatat tgtcatgggc tgtttgctag
     7141 ttatagagat ataaataaga tacttaatat agatagtcta aatataaaat ctagttcaga
     7201 attgattaaa aaagagtatg atatagatat tgtagctgga actatgagaa gatttgatga
     7261 tatgaataat agatttataa agggatatat atcaaatgat gatggttatt ttgaaagtag
     7321 ggagtataag ctagaaatat atgacagggt tggtgccgga gatggatatg tagcaggaat
     7381 tatatattca tatttggaaa atatgaagag tgaagatata gttgagtttg gggttgctag
     7441 ttcggtgtta gctcatacaa cttatgggga taattcttta gtaagcacag gtcatgtact
     7501 tagactgatg aataatgaaa aaatagatat attgagataa tacatttgta tataataaaa
     7561 tt
//
LOCUS       DAYAOX010000179         7554 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.180, whole genome shotgun sequence.
ACCESSION   DAYAOX010000179 DAYAOX010000000
VERSION     DAYAOX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.180"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            118..2262
                     /locus_tag="ACGI57_002917"
     CDS             118..2262
                     /locus_tag="ACGI57_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFG9717981.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            2350..2685
                     /gene="spoIIAA"
                     /locus_tag="ACGI57_002918"
     CDS             2350..2685
                     /gene="spoIIAA"
                     /locus_tag="ACGI57_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HFG9717982.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2699..3127
                     /gene="spoIIAB"
                     /locus_tag="ACGI57_002919"
     CDS             2699..3127
                     /gene="spoIIAB"
                     /locus_tag="ACGI57_002919"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042174 - negative regulation of
                     sporulation resulting in formation of a cellular spore
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HFG9717983.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3150..3914
                     /gene="sigF"
                     /locus_tag="ACGI57_002920"
     CDS             3150..3914
                     /gene="sigF"
                     /locus_tag="ACGI57_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HFG9717984.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            4194..4631
                     /gene="spoVAC"
                     /locus_tag="ACGI57_002921"
     CDS             4194..4631
                     /gene="spoVAC"
                     /locus_tag="ACGI57_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HFG9717985.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            4798..5811
                     /gene="spoVAD"
                     /locus_tag="ACGI57_002922"
     CDS             4798..5811
                     /gene="spoVAD"
                     /locus_tag="ACGI57_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HFG9717986.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            5825..6187
                     /gene="spoVAE"
                     /locus_tag="ACGI57_002923"
     CDS             5825..6187
                     /gene="spoVAE"
                     /locus_tag="ACGI57_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HFG9717987.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            6370..7386
                     /locus_tag="ACGI57_002924"
     CDS             6370..7386
                     /locus_tag="ACGI57_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9717988.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         3026 a          764 c         1400 g         2364 t
ORIGIN      
        1 ttttgataaa atatagctat gttttatata ctgtagtttt gaacatagaa agtttaataa
       61 attatagtat atatgttaat ttttaataag gtaagaaagt agaaagaagg atattttatg
      121 gatataataa aaaaattaag tcatgggtgt acattagatt gtcatgactg ttgtaaattc
      181 aatgtttata ctaaaggaaa taatgtagta aaaattgaag gagataaaaa tcatccttat
      241 acaaaaggtt ttatatgcaa aaaagggatg gcccatttag atagattaaa tcataaagat
      301 agaataaaga ctcctatgct taaagttgat ggagtatgga aagagatttc ttttgataaa
      361 gctatagaaa taatggcaga aaagcttaca tactataaag aaaagtatac atcaaagtct
      421 gtcatgcatt atgaccaata tggaagtggg tctgtgctaa aatacatagg agatatattt
      481 tttaattttt atggaggtgt tagcagacat aaaggtggtc cctgttggag tgcagggatg
      541 catgctcaaa aatatgattt tggagtagct aagagtcatg ctatagaaga tatgttaaat
      601 agtaaaagta tatttgtgtg gggaaaaaat ccagcatata ctacaattca tactatgcaa
      661 ataattaaaa aagctaaaga aaaagggata aagatagtag tgattgaccc catatataca
      721 aaaacagctc aaatagcaga taagtatgta caagtaaatc ctggaacaga tggagcttta
      781 gctatagcta tggcaaaaat tatagtagaa gataaattat atgatgaaga atatataaac
      841 tcgtatgtta ttggttttga agaatataaa aaatatttat cttcattgga attaagtttt
      901 ttaattgatg aatgtggtgt taaagagaat gatataagag agttagtgga tttatatact
      961 aacaaatatt caagtataaa tgttggatat gggcttcaaa aatataaaaa tggtggaaac
     1021 acaataagag caatagatgc tttaggtgct ataactggtc aaataggatt tagtggtgga
     1081 ggggtaaact atgctaataa agtttatccg agtgttataa attctgaccc ttacaatagc
     1141 caatcatatg gtgaagatag ggagttttat gtaagtaata tcagtaaatt tatagaagaa
     1201 tctttaaaaa atacatcaaa taaagtaaat tatgcatcag atgaacttga tatgacatca
     1261 aataaagtaa attatgtatc agatgaactt gatataacat caaataaaac agattatata
     1321 tcaaatgaat tgtataattt atctaataag tcaatcaaag gtaatattcc tataaagatg
     1381 gctgtaataa ctaaaagtaa tatgctgaat caactgccag atttagtaga attggagagg
     1441 gtgttttcaa aaattgaatt taaagtatgt tttgatatgt ttataactga tacagctact
     1501 ttatgtgaca tgtttatacc gtgtacaaat acactagaga gtgaagatat aatttatagc
     1561 tcaatgacca atccatatat aacctataat gaaagagctg taaagccagc acataaattg
     1621 atggatgaat attacttctt tatggagcta gctaaaaaga tgggattaaa tgattatcct
     1681 tttgtagaaa agagaactta tttagaaaaa gtgattgaac cacttaagcg ttttgacaaa
     1741 aatctggata tagaaaaact taaaaacaat tactttacta tacataaccc tgttgcttgg
     1801 gaagataaga agtttgagac tccatctgga aaatacgagc tttattcaga aagtataaaa
     1861 aacttgggaa taagtccaac acctgtatat ataagcaata aatataaaga aatagaagat
     1921 aaaaatattt cttttagatt attaactaat catcatgcag atactttatt tagtcagcat
     1981 tttatggaca aaaaatctat agctcaagca tatataaatc ggagaatggc taaaaaagta
     2041 ggtattgagg acaaagatat tgtgatattg aggtcaaaaa aagcaaaaat aaatgtacaa
     2101 ataaatatag atgatggagt aggtaattat atagtaaaaa tgtatgttgg ttggtggaag
     2161 aaacatggaa atcctaatag tttaacagac actggtatat cagattttgg gggtcaggta
     2221 acatataatg aaagtatggt agaaataata cgacaaaact agttaaattt cattgaagga
     2281 ataaaaatat aattatagaa ttgattaaaa gttactattt ttacaaaact attgaaggag
     2341 ggatttttga tggtaaatta ttccttagaa cataagaatc tatacataga atttatgtgc
     2401 tcagaactag accaccatgt agccaatgaa ataagggaag aaatagataa cttattaagt
     2461 gtaaatcaag taaaaaatgt tgtatttaat tttgaaaata ttaattttat ggattcttca
     2521 ggtataggcg tcatcatagg tagatataaa aagatttcta atgaaggggg acgagtttca
     2581 gtaattaata taagttcacg agttaagaag atttttgatt tatctgggtt aaataaaatt
     2641 attggcattt atgatacgta tgaagaagct ctaagttctt tgtagggagg agtatgtgat
     2701 gaataatatt atggaagtta aattttcggc aatatctgaa aatgaaagtt tagcaagagt
     2761 tatagtagca tcctttgcag ctaagttaga cccaacagta gatgagttag tagatataaa
     2821 aactgctgtc tctgaggctg ttacgaatgc tataatacat ggatatgaag aagacagttc
     2881 taagtttgtt ttcttaagat gtgagataga aggaaatact ataaagatta tagttgaaga
     2941 tgaagggtat ggaatagaaa atgtggagaa ggcaatggaa ccactttata catcaaaacc
     3001 agaacttgat agatcaggaa tgggattcac agtaatgaaa agctttatgg acgatgtaga
     3061 ggtaagttct gtaaaagata atgggactag aattgaaatg actaaaaaaa taaatgtgcc
     3121 taaataaagt ccaaaagtga ggttttatta tggaagtaac tgttgccaga gaagaaaaaa
     3181 aacctcttct tagtcatgaa gagactctag aacttattga aaaagttcaa aatggagatg
     3241 aagaggcaaa agaaatatta atatcaagca atttaggtct agttaggagc gttgtatcta
     3301 gatttttaaa tataggatat gatagagaag atttatttca gttaggttct ataggcttaa
     3361 taaaatcaat atataaattt gacccaaaat ttaatgtaaa attctctact tatgcagtac
     3421 caatgatttt aggtgaaata aagagatact taagagatga tggtatgata aaagtatcaa
     3481 ggtcgttaaa gcaaatagct ataaaggcaa agatggaaag tgaagcttta actaaaaaat
     3541 taggtagaga gccgagtata gaagaattag ctaaagcaat agatgtagaa aaagaagatt
     3601 tagtaatggc tatggaagct aatttcaatg ttgagtattt gcaaggagtt attcatgaag
     3661 aggaaggttc tccaatatgc cttattgata aaataagcat gaaaggagaa agtgaagaag
     3721 aaaaagtagt ggataacata ttgttaaaag acatacttgg aagactggat aagagagagc
     3781 gtcaaataat agttctcaga tactttgaag acatgactca aagtgaaata ggagagatgc
     3841 tcaatatatc acaagttcaa gtatctagga tagagaagaa agtattgtct aagttaaaag
     3901 aatatatatc gtgataatac ttatgatatg tagaataata aaatataata aatatattac
     3961 taaaataaaa tgtaataact ttttatgtta agtttttaaa ttattaaaag atattaaaat
     4021 aataaaaagc attaaaaaag taaaaatata aaaacaaggt gtctttatgg aaaaatagat
     4081 tttttcttta ttaggcatct tttttatttt ttgaccaaac tttagatagt caaaataaat
     4141 aataggttta tttttttaaa attaacaaat aatactttag agaggtgttt agtatggata
     4201 aaaattataa aaaatatgta gaccaaataa gcccaaaacc tacatattta aaaaactata
     4261 cacttgcatt tattgtaggt ggaattattt gtatgatagg gcaagcaata aatgatttgt
     4321 atatgaaagt tggaggtctt gataaactag gtgctagttc tgcaacatca ataacattaa
     4381 tatttatagg ggcattttta actggattag gagtttacga cctaatagga aaaagagctg
     4441 gagctggttc tataattcca ataacaggtt ttgctaactc aatagtatct ccagcaatgg
     4501 agtataaaag agaaggctat gtattaggtg taggtgcaaa cttatttaaa attgcaggac
     4561 cagttttagt ttatgggata ggttcttcta ttttatgtgg aattatttat tatattttta
     4621 agatgtttta gatgattttt atttaattta aattttatta attatgaaga aggtataaaa
     4681 tctgaaaata gctactaaag taactaattg aaaaataatt ctgcagattt aattaatatt
     4741 tttaattaat attaaaaatt tgaatagtaa aatgcaaaat taaaaaggtg tgaaaatatg
     4801 aaaaataaaa gaattggaaa aagaacagtc aagttagaaa ataaaccaac cataatatca
     4861 actggaacta tagtaggtcc taaggaaggt gaaggaccac taaaggacta ttttgatatg
     4921 attatgactg acgatttata tggtgaaaag acttgggaat tagctgaaag taaaatggta
     4981 gagactgcat ctcaacaagc tatccaaaag gcaggtaaaa aattgtcaga tgtaaattat
     5041 atgctaggtg gcgatttaat aaatcaaata gttccagcat cctttgcagc aagagaactg
     5101 gctattccat ttttaggtat atatggagct tgttctacaa tggcagaagg tctttgtata
     5161 ggttctatgc ttatagatgg tggttttgct gatttagtac tgtcaggtac atcaagtcac
     5221 tattgtacag cagaaagaca atttagattc ccacttgaac ttggaaatca aaaaccaatg
     5281 acagcccagt ggacagttac aggagcagga agtgtattat tagctcctaa tggagacggt
     5341 ccaaaagtaa agtatgttac tgttggtaaa gtaatagatg agggtattga tgatggtaat
     5401 aatatgggac cagctatggc tccagcagca atagatacta tctactctta ctttgaagat
     5461 actaatgatg acccaaatag ctttgatata attgctactg gagatttagg tactcttgga
     5521 aaacagatag cagaagactt tctaaaagaa aaaggtgtag atatatcaaa agtttatact
     5581 gactgtggta tagagatgtt taaccttaaa gaacaagatg tccactgtgg tggaagtgga
     5641 tgtgggtgtt cagctacagt gtttgcagga tatatatatg ataagttgag aaaaaaagaa
     5701 tttaacaaag taatgcttgt atcaacagga gcactacttt caccgacaag tacactacaa
     5761 aagcagacaa taccatgtgt tgcacatgca gtagtaatag taaatgagta ggaagtgata
     5821 taatatgtta atggactatg taagagtatt tattgtaggt ggaattatat gtttaatagc
     5881 ccaagtaatg atggattatt tcaaaatgca aactccatat gttatggtaa cgtatgtaac
     5941 aactggtgta gttttaactt ttctaggact ttatgagcca ttagttaagt ttgcagcagc
     6001 aggtgcaaca gttcctataa taggatttgg atattcactt gctacaggag taataaaatc
     6061 aattcaaagt gatggttttt taggtatttt tttaggaggt acaactgcta cagctggtgg
     6121 aatagcagca gctatattct ttggatatat tatgtcagta gtatttactc caaaagcaaa
     6181 accataaatc tttattaaaa gaataataag tttttgaaat aataactttt tataaagcag
     6241 gtggatatct tttagattcc acctgttttt tatataaaga agcgtttaca taattgaagt
     6301 tgacaattat ttacaacata gtgtagtatt atattatcaa aataaaatga agtgaagtag
     6361 gggataaaga tggtaactat taaagatatt gcaaggaatc taggtatcag ttattcaact
     6421 gtttctaggt gcttaaacaa taatcctaat gtatctgaaa aaacaaaaaa gaaagtggta
     6481 gaagaggcaa atagactagg gttccatttt aatgtaaatg caagaaattt agcaaaaaaa
     6541 gaaactaaca ggataggggt aatcttttca aataatttta atcatcaaga tactagaaag
     6601 ttttttagtg atataatgga tagttctata aattctattg agactaataa atacgatttt
     6661 ataatacagc ctaataataa cattagtggt gatagtaatg tatataaaat ggtaaatgga
     6721 caaatggttg acggattagt aattgtgtct caatcaataa aaaaagaaga gtatgatttt
     6781 ttaaaagaca ataattttcc acatgtattt atatatttta aaccatcttt tataggagaa
     6841 gtagataatt tcttttggga cgataacgtg tatggaggtt atatagctac caagcattta
     6901 attgaccatg gtcataaaga tataataaca gtaacttcag atgataagag ttcaaaaatg
     6961 catgaagata gaaccaaggg atatttgaat gctatggaag aagcaaattt aaagacagaa
     7021 gtcattaaat ctaaaatgga ttttgaatct caagttgaat ttttagaaaa atacattgat
     7081 aaaataaaaa aagcttcagc tatatttgta caacaagatg taccagcgat ttctataata
     7141 caagagttaa aaactactta tggtataaat gtaccagaag atatttccat aatagggtat
     7201 aataatattg aattaatatc atattttagg tcgcatttat caacaataga tgacccaaga
     7261 gagcaagtta taaaaaatgg agtagatagt ttagtaaata taataaataa aaagagtaca
     7321 gaacacattc caagaaaatt atatccaagg ttaataatac gaaatagcgt taaaagaata
     7381 aagtagtaag cttttaagta tcaaacgttt attaaaaata ttatagatta tgtgaagttt
     7441 tttaacaaat aatataaata aaaattaaaa ggagtaggtg tatataagta cattgtactc
     7501 ctttatttat tgagatagtt tttataatat gaatgaataa ttttattcaa ttat
//
LOCUS       DAYAOX010000180         7529 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.181, whole genome shotgun sequence.
ACCESSION   DAYAOX010000180 DAYAOX010000000
VERSION     DAYAOX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.181"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(45..974)
                     /gene="pfkB"
                     /locus_tag="ACGI57_002925"
     CDS             complement(45..974)
                     /gene="pfkB"
                     /locus_tag="ACGI57_002925"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFG9717989.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            1243..1968
                     /locus_tag="ACGI57_002926"
     CDS             1243..1968
                     /locus_tag="ACGI57_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9717990.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            2051..3205
                     /gene="nagA"
                     /locus_tag="ACGI57_002927"
     CDS             2051..3205
                     /gene="nagA"
                     /locus_tag="ACGI57_002927"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFG9717991.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(3914..4384)
                     /locus_tag="ACGI57_002928"
     CDS             complement(3914..4384)
                     /locus_tag="ACGI57_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFG9717992.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIY"
     gene            complement(4988..6145)
                     /locus_tag="ACGI57_002929"
     CDS             complement(4988..6145)
                     /locus_tag="ACGI57_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFG9717993.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            6299..6868
                     /locus_tag="ACGI57_002930"
     CDS             6299..6868
                     /locus_tag="ACGI57_002930"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFG9717994.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            complement(7050..7466)
                     /locus_tag="ACGI57_002931"
     CDS             complement(7050..7466)
                     /locus_tag="ACGI57_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717995.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
BASE COUNT         2669 a         1102 c          905 g         2853 t
ORIGIN      
        1 ctattacaac aacattaaat ttattatatt cccattaaat gtatttaact taactttttt
       61 acttcaacta aatcttgata tttatcaatt tctcccaaat ctacaaatcc agtttccata
      121 tttaaagcat tacttatacc acaagcattt gcaaatttaa gtaaattttc tattttataa
      181 ccccttaaaa tacctacact aaatccagcc actgtgctat ctccactacc tacagtattt
      241 atacacttta caactggtac ttttacatca tatacattat cttcgtttaa atacaccata
      301 ccatcttttc ctaaagatat acatacattc tctgcaccca tttcaataag tttttttcct
      361 gacaagataa tttcctctct tgattctata ttcattccta ataaatgtct tatttcatct
      421 atatttggtt ttattaaata tggttttgat tttaaagcta tttttaatgc ttctcctgat
      481 gtatctaata taaactttat attatttgct ctacatatat ttcctatctt ttcataatag
      541 tctatcggca tattctcaca ataactacca gaagctacca gtattttagt attttttagt
      601 acttcctcta tatcattttc aaatctctct aaatcgcttt tttctactat tggtcctttt
      661 tctaaaaatt ctgtactaac tttattgtcc tcaattatgt ttaagcaatt tctagtttcc
      721 tgctctattt ttgtaaattt attttgtata tctagttttc taagttcatt ttttataaat
      781 tctccattaa atccgcctag aaatcccata gctaaaggat tttcttttaa aattttacat
      841 accttagcta cgtttaatcc tttacctcca gcagttgcat tcttacttac taccctttgc
      901 acctcaccta tgtttatatt attcactcta tacattctat ctatagatgg gttgaatgtt
      961 attactgtaa tcatactaga ttctcctcat tattaatttt tacttttcta aacaataaat
     1021 taatctgtaa aataaaaaat acctcatcta taatataccc aaagtggtta ttaccaattt
     1081 gaatataaat tattttactt gttttgtcaa tgtaccataa ttttataata aattaataac
     1141 cttacttatt gaattttgat taaactttat tcattgacat aaataattct atattctata
     1201 atcaaattgt ggtaataacc acttagaaag gactatattt ttatgataaa aagaaatgat
     1261 aaaagaccaa tatatgacca gcttgttgag atattaagaa ctaagattga aaatgagatg
     1321 gaaccaaacg atagaatgct ttccgaaaga aaaatttgtg atgaatatgg agtaagtaga
     1381 accacagtta gactagctat ggctgagttg gaacatatgg ggtatatata caaaagacat
     1441 ggaaaaggaa cttttgttgc tgcactaagt caaaattctc aaaacttaat ggaaagttat
     1501 agttttacag attatatgag aacacaagga aaaaagccag gtacaaaggt attatcattt
     1561 gaggtagtag aaagtacaaa gtatttctct gaaaaattag gtataactcc tggtgaaaaa
     1621 atgataaaaa taatgcgttt aagacttgct gatgaccttc ctatgatgct tgaaagaaca
     1681 tatcttccta tgaaagaatt tataggattg acaaaagaaa aaatcgaaca aaaaccttta
     1741 tatgaaatat ttagagaaga ttatggcgag attattaaag ttgctgatga agaattttca
     1801 gctggaataa tctctgataa ggaagctaag ttattggaag taccaggaga ttcaccttgt
     1861 ctgaaattac ttcgtaccac atataatgat gacaatatag ttattgaatt tactttgagt
     1921 gttgctagaa gcgataaatt tgtatataga gtaagacatg taagatagta agttaaataa
     1981 ctttcaagaa aatagaatga tattataaac gtcaatgcca caatgatagt ataattataa
     2041 ggaggttatt ttgaaaagtt ttatttatgc agataaattt tttatgaaac atggaatcaa
     2101 agaaaaaggt taccttagta taattaatgg caaattcggt gaatttcaac aagaaagacc
     2161 taatgttact gataatgtta tagatttttc aggtaaatat attgctcctg gcttagttga
     2221 tactcacatt cacggtcttt taggtgcaga tgtcatggac aatacatttg aagcaataaa
     2281 cacaatttca aaaggcttat taaaatatgg agtaacatct ttcctaccta caactttaac
     2341 tgattctgta gatacactaa gtgattctgt agaaaatata agcaatagct tcaatcgagt
     2401 tgagggtgca aaaatacaag gtatttattt agaaggtcca ttttttacag aaaaatataa
     2461 gggcgctcaa aatgaaaaat attttaaaga ccctgacatc aatatattaa aattatggca
     2521 aaaaaaatcg aaaaatttaa taagaaaaat agcaattgct ccagaacgta aaggttcaat
     2581 agaattaaca agatatgcaa ctaaaaataa tatatcagtt tctcttggtc atagttcagc
     2641 tacatttgag caagttaaac aagtcgttaa ttcaggtgct aaagtgtttg tacatacata
     2701 taatgggatg agtcctttaa atcatagaga acctggaatg gttggggctg ctatggtact
     2761 aaaagatact tatgctgaat taatttgtga tggtcatcat gtaagcccaa tagctgttaa
     2821 aataatgatg gatgcgaagt ctagtaataa catagcttta attacagatt gtatgagagc
     2881 tggtgctatg gaggatggaa aatatactct tggtaagttt aatgtaaatg taaaaaatag
     2941 tgttgcaaga ttaaatagtg ggagtttagc tggaagtgta ttaactatgg acaaggctgt
     3001 gagaaatata gttgattgga acatatcaac tttagaagat gcaataaaaa tggctacata
     3061 tgtccctgct ttaagttgta atattgataa tgtctgtggc tctatatatc aaggtatgga
     3121 tgcagacttt atagtaacag ataatgactt taatatacac gaaacttata taaatggtat
     3181 ctgtaagtac aaagttaata aataggttca atataaggtt atggaggctg tctaaaaaga
     3241 gtctggaaat atttaaagtt atttctccaa aatctgattt aacactttta tttccatatc
     3301 gttaataaac tattgcgata gaggttattt taaacaaata tatattataa gtatttttgt
     3361 gataatattt tcgataatat gtacatttaa agtaactttt ctattattat ttgtaagttt
     3421 ttatcttgag caaacatata aatttttaat aaagcatgag ataaacccta tatttcaatt
     3481 actaatgatg agattttgat tgataacaga tatattttca aatgtaaaaa tactaaaaat
     3541 catatcaatt ttaaagatat tgtaggtgta aataaacaag ataatactat agaaattttt
     3601 acaaatgaaa gcacttattg tatatatcta aaagttttaa gcattgagag cgaagaatat
     3661 ctaatagata gactaaaatc attttgttga ttttatatat tttatgttaa atataaagca
     3721 gagtagttaa aactaccctg cttttgtaat taaaatttaa aaataataac tcataataca
     3781 ttgaatttga gcttaacagt aactattgta ataatagtat caatcttttg tgttgaatat
     3841 aatttattta cactatagtt aagcatgatt tctaatgtta ttttctctaa tttacaatta
     3901 tgattaaaat tcattaatat atatatttat aagttgtatt gtttatattg tttattactt
     3961 ttttaaatcc actagctgta ataataaatg tcttttctgg tatccctggt gaaaagatta
     4021 ttttatcatg gttaaatata tcttgatcta ttacaagaga gatatttttc tcatatccaa
     4081 aaggagatac acatccaggc aaacattgtg ttttttgtgt taattcttct tttgaacata
     4141 tagatacttt tccacctaaa acatttttta tagtttttgg atctaatttt tttccttctg
     4201 tggttacaaa tatatagtaa tttccagatc tatcttttat aaataaactt ttactttcta
     4261 ctccagtata ttgtaatttt tctcttattt ctttagcttt ttcaaatgat aaaataggtt
     4321 catggtcata ttcttcaaac gttatatgat tttcttttaa aatatttttg atattgttgt
     4381 acatatattt tcctttctat atttaaatta atttatatat aatattcaat tataaagtga
     4441 ttttataatt tcttcaaact atcataaaaa acattcacat gttttaggaa taaattatat
     4501 aaaataggtt ataagataat gaacatttat attataacct atttttatat cgataagtat
     4561 gaaattgttt aaccaagtca aaatgatata taaatttata tttaaatgtg tttagagttt
     4621 aatttatttt caatgtttaa aaaagttatt ctatgcatct aatttaatta gtaaactgag
     4681 gttctagtta gataaattat attataatga atatatagta tgcagtttac gatattttaa
     4741 aagtaaatga tataatattt ggaacaactg ggttgcaatg tgtttttaaa aaatatcaaa
     4801 ataaggctat gatttttaaa accatagccc tgtctgtaaa aatttaatta tcacttaaaa
     4861 aataaatttt tatttaaaga atacacataa atatttccag actccctcat gaactaaaaa
     4921 gttcattttg agacatctct tttataatgt tctttatttt atagtataag tttataaaaa
     4981 ttaaatccta tttatcaatc tgttctctaa gccacttagt tgctacatct aactgtttat
     5041 tttcatcttt ttcttcaata gtaggcttta tacctttttc attgatataa tctccattag
     5101 gtgtaaaata ttgagcagta gttaacttgt atcctgttcc atccttcaat cttacaactg
     5161 attgtaccaa ccctttacca aaagttgtag tccctacaga aataccttct ttattatcaa
     5221 cgattgcagc tgttaatatt tcagcagcag aagcactttc tccattagtt aacactacta
     5281 ttggaagtcc caatttttct ttatcagatt tcaagtactc agtatttccc ttattatctt
     5341 ttgtatatac tatagttcct tcaccaatta actcatcagc aacttcttta catatatcta
     5401 gcattccacc aggattattt ctaaggtcta ttactaatcc cttgatattt tttgctttta
     5461 attcttttaa agctttatca aagtcagtat atgtacttga tataaattgg gtaatttcta
     5521 tatatcctaa atcattatca attactttac tttctatact cttctcaatt atctggtctg
     5581 tttttacttt aaagttcaat tgtttatctt ctcttaatat agtcagttca actgttttac
     5641 cttttttacc cttcatcata cgaacagctt catctgaatt tttatatgag acagattttc
     5701 cattgacttt tactaattta tcccctgatt ttacacctgc tttctctgct ggagaatctt
     5761 tcatgggaac tactactaag ccaccatctt ttgatgcccc tatatacata cctacaccaa
     5821 cataagaacc ttcactatca ttaattagat tctccatttc atctttagta taataaccag
     5881 aatacttatc atttgttcct agaaatagac cttttgctgc tccatttacc aaatcagtat
     5941 ctgatacttt tttataaaaa tcttgtttta taatagattc tagtcctaac aacttatcat
     6001 attttttata gtcttcatag agttctttcg atattacaac tttattcccc aatgtcaact
     6061 gaaatgctga tgtaaccata gctgttataa ttactaaaat cacccctaaa aaaatagctt
     6121 tcttctttga aatcacaaga tacatctcct tttacataaa ctattattat tatatgacta
     6181 gctgttattt ttattacatt tacccaaata tattgcttta ttacaataaa ttatactaaa
     6241 atttatatta aatacaattg ctgtacacat tataacatat taagggagga attattttat
     6301 gaaaaaagaa tttagaaaaa aagttattga atcaagaaaa aataagagtg caaacttcgt
     6361 acttagtaat tcaaatttaa tcactgaaaa attacttcaa atggaagata taagaaatgc
     6421 aactaatata atgctttatc taaattttaa caatgaagtt caaacagata atttaatcaa
     6481 aaaacttttg tctctaaaga aaatagtatc aagtcccatc acaataaaag aaaatcatac
     6541 attaatccct actcaaataa cagatttaaa agagggattg agaattggag catatggaat
     6601 aagagaacct aacgaaaaat cacctacaat tgatattaag gccttagatg ttgtaattgt
     6661 accagctgta gcatatgata tacattgcta tagattagga tatggtggag gattttatga
     6721 tagattttta gaaaacttaa gaaaagatgc tataactatc ggcatagcat ttgacttcca
     6781 gatattcgat tctattccaa aagaaactca tgatgcacaa ttagactaca tagttactga
     6841 aactagaatt atcactccaa ataattagaa tattaagtat ttccagaccc catttaatat
     6901 agtaataaat actactgtta atatcttact ttataagcat tttaagtcat ggcttttaca
     6961 acaataagaa ccatatacaa tttacttaca ttaataaaaa ccatgacttt attgtacaat
     7021 atgcagttat ttaattataa aataccttct tatttcgtcg catttaattc tattctatct
     7081 ggaagttcac tcatatttgt ttcaacaact atgtatggaa aagaatcttc cttatcttta
     7141 tactttatct tgactctcaa aacatccttt ccttcctcaa ccacactttc tattttgtcc
     7201 atttctatac catatttatt atttcctcta gtcactataa tgtatatttt gttatcgatt
     7261 ttagccgcta atgctctttc ttcatttgta tacttatcca tcacatctaa tatcttttct
     7321 ggtattgaat ttctttgtaa tactgtataa tctatgggtt tagatttctc tacaaacata
     7381 cttggtatga atataaatcc ccctataacg aatacaagag ctataactcc ataaattata
     7441 cctttaatat taaactttac attcaatctt atcactcctc ccatatattc taaatatatg
     7501 atttataaac ttatcttaga ctctaaatt
//
LOCUS       DAYAOX010000181         7512 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.182, whole genome shotgun sequence.
ACCESSION   DAYAOX010000181 DAYAOX010000000
VERSION     DAYAOX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.182"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            253..1734
                     /gene="gltX"
                     /locus_tag="ACGI57_002932"
     CDS             253..1734
                     /gene="gltX"
                     /locus_tag="ACGI57_002932"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFG9717996.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            1955..2110
                     /locus_tag="ACGI57_002933"
     CDS             1955..2110
                     /locus_tag="ACGI57_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9717997.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            2255..3652
                     /gene="cysS"
                     /locus_tag="ACGI57_002934"
     CDS             2255..3652
                     /gene="cysS"
                     /locus_tag="ACGI57_002934"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFG9717998.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            3655..4050
                     /locus_tag="ACGI57_002935"
     CDS             3655..4050
                     /locus_tag="ACGI57_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HFG9717999.1"
                     /translation="MEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRKVNDLHK
                     LAIKYVKAKAQATIIHEVEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKHATGFEA
                     LVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            4156..4914
                     /gene="thyX"
                     /locus_tag="ACGI57_002936"
     CDS             4156..4914
                     /gene="thyX"
                     /locus_tag="ACGI57_002936"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HFG9718000.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            4927..5664
                     /gene="rlmB"
                     /locus_tag="ACGI57_002937"
     CDS             4927..5664
                     /gene="rlmB"
                     /locus_tag="ACGI57_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFG9718001.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            5667..6194
                     /locus_tag="ACGI57_002938"
     CDS             5667..6194
                     /locus_tag="ACGI57_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFG9718002.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            6259..6906
                     /gene="sigH"
                     /locus_tag="ACGI57_002939"
     CDS             6259..6906
                     /gene="sigH"
                     /locus_tag="ACGI57_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HFG9718003.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            6980..>7512
                     /locus_tag="ACGI57_002940"
     CDS             6980..>7512
                     /locus_tag="ACGI57_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFG9718004.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSAL"
BASE COUNT         3162 a          759 c         1452 g         2139 t
ORIGIN      
        1 tatatagatt ttcaataaag caaacaataa atataaatga atattaataa ggagtgcttt
       61 taatttttag aaaataaatg acttaaaaga aagatttatg aaattaaaac taggattttc
      121 tgatataata aacatgtgta tttttaaata aaaaggatat tattattaga tttgattaaa
      181 aataaacttg caaaacaatg tactagttta atattatagt tatattgaat aaaatatcag
      241 gaggaagtaa aaatgaatgt aagagtaagg tttgcaccaa gtccaacagg atttgtacat
      301 ataggtagtt tgagaactgc tttatataac tatttatttg caaaaaaaat gggcggagaa
      361 tatatattaa gagtggaaga tacagaccaa agtagattag tagaaggtgc tatagagaat
      421 atgcttaatg ctatgaaatg ggctggggtc aaccatagtg aaggtgttat attagatgat
      481 agtggaaaag tagtacaaaa aggagaatat ggtccatata ttcaatctca aagattagat
      541 atataccaag aatatataaa gcaactttta gatagtggaa aagcttatta ctgtttctgt
      601 acaaaagaaa gactggatga agtaagagat gctcaaagag cagcaggaga aacagctaaa
      661 tatgatggtc attgtaagaa tctttctaaa gaagaagtag aagcgaatat aaaagcagga
      721 ataccatatg ttataagatt aagattgcca gagaatcata caattaagtt tacagactta
      781 gttagaggag atatggaatt taatactaat gacttagatg accaagtatt aatgaaaaca
      841 gatggatttc caacttatca ttttgcagta gttgttgatg actatttgat gaaaattaca
      901 catgttataa gaggagaaga atgggtatca tctactccaa aacatgttta tttatatgaa
      961 gcttttggat gggaagcacc tgtatttgtg catttaccaa atatacttaa taaagaaaag
     1021 aaaaaattaa gtaaaagaca aggtgatgta gctgttgaag actttaagaa aaaaggatat
     1081 ttaccagagg gattagtaaa ctatgtagca ttagttggtt ggtctcctga agataatcaa
     1141 gaattgttta caatggagga attagaaaaa gccttctctg tagaaagagt atctaaaagt
     1201 ggtggagtat ttgatactga aaaattaaat tgggttaatc agcactatat aaaagatggt
     1261 gatgatgctt atctaacaga cttagcaata ccatttttaa tagaagatgg atttataaca
     1321 gaagaagaag ctactaataa atatgatttc ttaaagagta tgatatcagt acttaaagag
     1381 aaattacaat atgtaaaaga agttactgaa catgcaagta tattttttgg agataaaata
     1441 gaggtagaaa ctgaagaggg aagagagttc ttaagattag aacatatacc tactttaata
     1501 gatgctttga gagaaaaaat agaaaaaaca gaggtattaa atgctgagtt tgttcaagct
     1561 atgcttaaag aaatacaaaa agagtacaaa ataaaaggga aaaatttatt tatgggctca
     1621 agaataattt tgacagggca aatgcatggt ccagatttac caaaagtaat ggaagtttta
     1681 ggaaaagaaa cttgcttaaa tagaatagct tatgtaaaaa ataatatatt atagtcataa
     1741 aagtccgcta atttagaagc ggactttttt tgtgttagta tttggtgaaa atatctgctt
     1801 aagaaaatga aggtatttaa ggtaataaat ttaatatata aaaagcaaag caaaaatata
     1861 aataaaatat gaataataaa ctaataaaat attaagcata gaagaaggga atctattatt
     1921 ttagtttgat ttaattatta agggggtaat tactatgtat agtaatattg atgatgttaa
     1981 aaaggaatta aaggagctat gcttagaata tgttacaatt ttagaaaaat taaaagatga
     2041 aaaaatgatt acagaagaaa cttttgaaaa gtgctcttct caaaaaaaaa tatttttaga
     2101 agaacaatag aggatggtaa tagtattatt attatgtagt ttgaaagata gtacgaaaaa
     2161 tgatagtaat actattattt attgcaattt tcaactataa tataatatat aatgttaaga
     2221 agataattta taaaaataca gtgaggtgat gagtgtgaaa gtttataata cattgactag
     2281 aacaaaagaa gaatttgttc cattagaaga aggaaaggta aaaatgtatg tgtgtggtcc
     2341 aacagtttat aactatatac acataggtaa tgcaagacca tttataatat ttgatactct
     2401 aagaaggtat ttagagtata gaggatatga tgtaacttat gtacaaaact ttacagatgt
     2461 tgatgataag attataaata gaagtcatga ggagggaata tctcctgaag aagttgcggc
     2521 taagtatata aaagaatatt ttgtagactg tgatgggcta ggaataaaaa gagctactgt
     2581 tcatcctcag gttacagata atatacaaca gattatagag tttataaaag agttggaaga
     2641 taagggatat gcatatgcag taaatggtga tgtatatttt gatacaaata agtttgaagg
     2701 ttatggaaaa ctttcaggtc aaaaacaaga agatttagag gcaggggcta gaattgaggt
     2761 taatgaccaa aaaagacatc ctatggactt tgttctttgg aaggcaaaaa aagaaggtga
     2821 gccaggatgg gatagccctt ggggagaagg tagaccagga tggcatatag aatgttctgt
     2881 tatgtctaag agatatttag gtgaaacaat tgatatacat gcaggtgggc aagatttaac
     2941 attcccacat catgaaaatg aaatagctca aagtgaagct agaagtggaa aaacattctc
     3001 aaaatactgg atgcataatg gatatataaa tataaatgat gaaaagatga gtaaatcaaa
     3061 aggtaatttc tttacagtaa gagatatatc aaaattatat gatttagaga tagtaagatt
     3121 tttcatgtta tcagcacatt atagaaatcc agtaaacttt agtgatgaga tgttaaatca
     3181 agctaaagca ggtcttgaaa gactatataa tactaaagaa aaattagaat ttacgctaag
     3241 taatttagta gaatctcctt taacagaaaa agaagtagag cttgttaaag aattagatga
     3301 ctttagacaa aaattcatag atgctatgga tgatgatgtt aatacagcag acgcagtaag
     3361 tgtaatattt gaattagcta agttaataaa ttctaatgta gatgaaaatt cttctttgga
     3421 gtttgctaaa aaatgcttag atgaatttaa tgaacttaca ggagtattaa atattgtaaa
     3481 taagaaaaag gatactgtat tagataaaga cattgaagaa ttgatacaaa aaagaactga
     3541 tgcaaagaaa aataaagagt ttcaattagc tgatgatatt agacaacaat tactagatat
     3601 gggaattgta ttagaagaca caagacaagg tgttaagtgg aaaaggatat aaaaatggaa
     3661 aaaactgaat tagttactat gtcaccatta gttttagcat acttaggtga tacagtgtat
     3721 gagacatata taagagaata tttaattaga cagaatacac aaagaaaagt taatgatttg
     3781 cataaactag ctataaagta tgtaaaagct aaagctcagg caactataat tcatgaagtt
     3841 gaaattgagt taactgaaga agagagtaaa atttataaaa ggggaagaaa tcaaaagtca
     3901 aatacttctc ctaagaatgc agatattata gactacaaac atgcaactgg gtttgaggca
     3961 ttagttggat atttgtattt aaataatgaa atagagagac ttcaatatat tatcaataaa
     4021 ggaatcaaaa ttattgaaag agatatgtaa atattaaaga gtgttgtctt aataataagt
     4081 aataaggcaa cacttttttt ataactaatg tataataaat atatagaaat tagtaatgag
     4141 aaaggataaa aagttatgaa agtaaaatta atatcacata cacctgaacc agaaaaagta
     4201 atagctatgg cagctaagct atgttattca ccagttggga cagacgaaat agaaaaagac
     4261 ttaacagatg aaagtataga gaaattttta aatatgttat taagtatagg tcatgggtca
     4321 atattagaac atgcttcatt tacattttca atagaaggta tttcaagggc atgttcacat
     4381 caaattgtta gacatcgtat agctagtttt tcacaacaaa gtcaaagata tgttaaatta
     4441 gaacaatttg agtacataat tcctccagaa atagaaaaaa tagaaaaggc aaaagagtta
     4501 tttatagact ccatgaaaaa agaccaagag aattatgata agttagttga aatattgttt
     4561 gaaaatcatt ataatgacct aataaaaaac ggtaaaaatg agaaaacagc taaaagacaa
     4621 gctgagaaga aagcaataga agatgctaga tacgtatttc caaatgcatg tgaaacaaag
     4681 atggtattta ctataaatgc tagaagtttg tttaatttct ttgaacatag atgctgtgaa
     4741 agagcacagt gggaaataag aaatttagct gtggagatgt taagagaggt taaaaaggtt
     4801 gctcctatat tatttaaaaa gactggtcca agttgtgtaa atggaagttg tccagaagga
     4861 actatgactt gtggagatat agttcaagtt agagaaaaat ttaaggcctt gtaggaggtg
     4921 aataatttgg cgagtataga aggaagaaat ccggtaatag aagcaataaa aagcgacaga
     4981 gaaatagata aaatattaat tgcaaattca gctaaagaag gttcaattaa gaaaataata
     5041 ggaatggcta aagataagaa tataattatt cagtatgttg ataaacataa gttagatgaa
     5101 gtaagtacaa gtcattcaca tcaaggtgta atagcttatg caagtgagta taagtattat
     5161 gaattagatg agctaataga tttagcaaag aataaagatg aagacccatt tttcattata
     5221 ttagatgaga taacagaccc acataatttg ggttcaataa taaggacagc agatgcagta
     5281 ggagcacatg gagttatcat tccaaaaaga aggtctgtac acataactcc tgtagttgcc
     5341 aaagcatctg ctggagcagt agaatatatg ccagtttgca aggtaaccaa tatagttaat
     5401 acaataaaaa gactcaaaga agaagggctg tggattgctg cagctgatat ggatggagaa
     5461 actttttata aacaaaatct tactggtcct cttggtgtag taattggaag tgaaggtttt
     5521 ggaatatcta gattggttaa acaaaactgt gactttatag taaaaatgcc aatgataggt
     5581 aatgttacat ctttaaatgc atctgtagca ggtggaatac ttttatatga gatattcaga
     5641 caaagactgg ataaaagtaa ataggcatga aaaaaaatat aaatcattac ttaataatag
     5701 atggctacaa tattataaat gcgtgggaca atttgaaaga actggctaaa gaggacttag
     5761 aagattctcg agaaaagctt attgatgaca ttatagaatt ttctgagttt atgggttata
     5821 agactataat agtttttgat gcttataatg ttaaaaattc tcgagaaaaa gttgaaaaaa
     5881 gaaaacacat aacaatagtt tatacaagag aacaccaaac agcagatagt tatatagaga
     5941 aatttataac ttctttatca aagtatgatg atgtaaaagt agccactaat gactatgcag
     6001 aacagcaaat gattttagga aaaggtgcta ctagaatgtc tgcaagagaa ttaaagttag
     6061 agttagatcg ttccaagaat aaaatgaagg aaaaaaatat cggtttgagg aaaaaaattc
     6121 agcgaaattg gttggaggaa aggttagata aagaaacatt gtcgaaactt gagaacattc
     6181 gtagaaagcg ttgaaattac taggttcttt agactataat atacctataa atttgtttta
     6241 tatatggggg agatttacat gttggtagca aaagaaaaaa gttatgagtt agtggacaat
     6301 tgtcagcagg atgagtataa cattgtatta agagcaagtg aaggggataa aatagcacta
     6361 gagtacatta ttactaaata cagaaatttt gtaaaggcaa aagcaaagtc atatttttta
     6421 attggagcag ataaagaaga tataatacaa gaaggaatga taggccttta taaggcagtt
     6481 agagattttg atggaagtaa gaccaattca tttaaatgtt ttgcggagat atgtataaca
     6541 agacaaataa taactgctat aaaaactgca acaagacaaa aacatatacc tttaaattca
     6601 tatgtttctt tgaataaacc aatatatgat gaagagtcag acagaacact tttagatatt
     6661 atagctacaa gtatagtaac agatccagaa gagcttataa ttagtaaaga agagttaaaa
     6721 aatatagaat caaaaatgaa cgaattgtta agtgacttgg aattagaagt actagaatta
     6781 tatttaaatg gaaagtcata ccagtttata gcggacaagc ttaaaagaga tgtaaagtca
     6841 atagataacg ctttacaaag ggttaagaga aaattagaaa aacatcttga aaacagaaat
     6901 gattaatagt aaaatactta ccataagctt attgtgagca aaattaattt atatcgaaag
     6961 ataagggagg aaattcaaaa tggctaaagc taaatacgaa agaacaaaac ctcatgttaa
     7021 tatagggaca ataggacacg tagaccacgg taaaactaca ttaacagcag caataacaaa
     7081 aacattatat gacagatatc aattaggaga agcagtagat ttcgcaaaca tagataaagc
     7141 tccagaagaa agagaaagag gaatcacaat atcaacagca cacgttgagt atgaaacacc
     7201 aaatagacac tatgcacacg ttgactgccc aggacatgct gactacgtta agaacatgat
     7261 aacaggagca gcacaaatgg acggagcaat attagtttgt tcagcaacag atggaccaat
     7321 gccacaaaca agagagcata tactattatc aagacaagtt ggagtaccat atatagtagt
     7381 attcttaaac aaatgtgaca tggtagatga tgaagagtta ttagagttag tagagatgga
     7441 agtaagagat ttattaacag aatatgattt cccaggagat gacactccaa tagtaagagg
     7501 ttcagcatta at
//
LOCUS       DAYAOX010000182         7441 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.183, whole genome shotgun sequence.
ACCESSION   DAYAOX010000182 DAYAOX010000000
VERSION     DAYAOX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.183"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            217..1392
                     /locus_tag="ACGI57_002941"
     CDS             217..1392
                     /locus_tag="ACGI57_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9718005.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            1427..2965
                     /locus_tag="ACGI57_002942"
     CDS             1427..2965
                     /locus_tag="ACGI57_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFG9718006.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            2997..4199
                     /locus_tag="ACGI57_002943"
     CDS             2997..4199
                     /locus_tag="ACGI57_002943"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9718007.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(4366..4965)
                     /locus_tag="ACGI57_002944"
     CDS             complement(4366..4965)
                     /locus_tag="ACGI57_002944"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718008.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            5135..6493
                     /locus_tag="ACGI57_002945"
     CDS             5135..6493
                     /locus_tag="ACGI57_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718009.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            6486..7118
                     /locus_tag="ACGI57_002946"
     CDS             6486..7118
                     /locus_tag="ACGI57_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFG9718010.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
BASE COUNT         2843 a          866 c         1292 g         2440 t
ORIGIN      
        1 aaaataaaaa aactcattat attaagataa aaatagaaaa ataactagaa aaatactata
       61 atatatcagc ttaatacaga aaaaagttaa aaaatttgag aaaaataaaa atgaaaacgt
      121 tttatattga catagagaaa aagtagtgat accatttaaa tatagtttag tagctaaact
      181 atatttaaat tataataact attttagggg gatgttatga atttaaaaca attaagtgaa
      241 aaatacgaag aatatgtaat tcaaatgaga agagaatttc ataagtatcc agaattatct
      301 tttaaagaaa taagaacaac ccaaagaata agggaagaat tacaaaaaat aggggtccct
      361 tttgtatcta ttgaaccaaa tatcgtaata gctactatac aaggcaaaaa tgaaggtaag
      421 acaattgcaa ttagaggaga tatagatgcg cttccaatgc aagaagattc agatgttgaa
      481 tacaaatcag aatatgatgg aataatgcat tcttgtggac atgatgctca tggagctatg
      541 ttactaggac ttgcacatat gctaaatgat ataagaaaag atttaaaagg tagagtatta
      601 ttagtttttc aagcagctga agaagttggt ggtggacaca aggagataat acagtacttt
      661 aaggaaaatg gaggaccaga tagattaata gctactcatg tttggtccga tattgaagct
      721 ggtaaaattt ctgttgaacc aggacctcgt atggctggtg ggtctggttg gagaatagac
      781 ataacaggta gaggaggtca tggttctaga ccagatcaaa gcatagatcc tataaagcca
      841 gcttgtgata tagttttaaa agtatcttct ataccagtta atcatataag tgtactagaa
      901 caatgtgttg tacatgcatg tgcaatacat ggaggaacta caataggaaa tgttattcca
      961 aacaatgcag aggtaatagg tggatataga tattttactg aagaatcagg caaaaaagtg
     1021 tttgacatta taaagcaaat gagtaatggt gttggacaag tttatggagt ggatgtgaag
     1081 gttactcata ctggaggcat aggacctgtt atcaatgatg aagaagcagt taagatggct
     1141 aaagaaatag tttctaatat agatggattg gagttagatt cttatgagcc aatatgtgca
     1201 tcagattgtt atggtgaaat attaaaagag tattctggct tttattgtta tctaggggta
     1261 ggtaacaaag aaaaaggaat aatatatgca caacaccatc ctaagtacaa tatagatgaa
     1321 gatacattaa agttaggttg cgaatttatg gctaaatatg cagttgaatt tttaaataaa
     1381 aataataact aaaataaatc gaattttata aatggggggg atgaaaatgc aaggtgcatt
     1441 aaaggaaaat aagaaaaaaa gtggtggagg atttttagga tggattgaaa aagttgggaa
     1501 taaaatgcca catcctctag cattatttac atatattaca ttgatagtag tagcattatc
     1561 tgcaatagca agctttttag gtttttcagc aacaagtcct gctacaggaa agttaataaa
     1621 agttatgaac ttaataagtg caaaaggttt agtactattt atgcaagagt ttgttaagaa
     1681 cttccaaaac ttccctgtgc ttggagtagt tttagttatg gcagttgcca caggagtttg
     1741 tgaaaagaca ggattttttt caactgccat aaaaatgagt ttatcaaaag ttaaggggaa
     1801 tgcggttgta tttataatag cttttatagg agtatttgct aatcaagctg gtgatgctgc
     1861 ttttgtatta gtaccaactc ttgcaggagc aatattttat ggattaaata gaaatccatt
     1921 agctggtata ttctgtggat ttgcatcagt tggtggtgga tttgcaacag ctgtaattcc
     1981 tgggggatgg gacgttacgc taacgccaat aactgtatca gctgctcaaa ctattcaacc
     2041 tgcatttgat atgacattat tagctagtta ttatgcacta tttgtatctg cgtttatagt
     2101 agctacagta tcaacaatag taacggttaa atttatagaa cctaaattag gtaagtatac
     2161 tggtaaacca gaaggtatag atgataatca aggttttgag gtcacaaaag aacaagcaaa
     2221 agcagcaaag ttagcaggtt ttacagtttt agcttatgta gcgttattaa ttgctttgtg
     2281 tataccagct aatagttttt taagaagtcc agaaggttct ttaatatttg gagcaccact
     2341 tatgtcttgc ttacagtttt ttatagtaat actattcttc ttgccaggga ttgtgtatgg
     2401 aatgagggtt ggtaaaatca agactgtaaa agacttaaat gatatattat gtcaatcaat
     2461 ggcagcaatg gcaccgttta tcgtacttgc aatagttata ggtcaattct taacgctatt
     2521 ctcagtgtct aatttagcac aggtactagc tataaaaggt ggagcattac tcaactcatt
     2581 gccactacca ccacaagcta taatactgct attcttagta ctagtagcgt ttataaatat
     2641 atttgttata agcggaagta caaaatggat gatatttgga ccagtatttg ttccaatgtt
     2701 aatgcaatta aacatacatc cagcatttac tcagtttgta tatagattag gagattctgt
     2761 aaccaatcat ttatcaccat taaatgcatt ctttataata cttttagcaa cagtccaaaa
     2821 atacgataaa aatgcaggta tgggaactat attctcagct atgatacctt acactatagg
     2881 ttactttgta gtacttgcag ttttagtagt agtttggatg acaataggta taccagtagg
     2941 aataggtgga caagtttggt tataaaacat atttaataat tttgggagga aagaaaatgg
     3001 aaacaactga aataagatac aattttgatg aagtaataga tagaagtgga acaaactgtc
     3061 ttaaatatga tcttaagagt caatttatgc cagaatctcc agaagatgca ctaccactat
     3121 gggtagcaga tatggatttt gcatgtgccc ctgagataat taatgcaatg catgaaagaa
     3181 tagacagaaa aatatttgga tatagctctc aacaaactga tgattattat gaagctgtat
     3241 gtggatggtt taagagaaga tttgattgga aaatagaaaa agagaatata ttctttagcc
     3301 caggggttgt gcctgcgtta ggatttttca cacaaatatt aacacaaaag ggtgatggta
     3361 tcataataga aaagcctgta tactatcctt ttgaagataa aataactaat aatgaaagaa
     3421 aagtagttaa tagtccttta aagtttgaag atggtcatta ttctatggat tatgatggat
     3481 ttgaagaatt agctaaagat gaaaataata aggtgtttat attatgtagt cctcataatc
     3541 cagttggtag agtatggaaa aaagaagagc ttagaaaaat agtagacata tgtaagaaat
     3601 atgatttgtg gataatatct gatgaaatac attgtgatat tattcgtaaa ggtgaaaagc
     3661 atataccatt agaagtagca tgtcctgatt ataaagatag aataataact tgtacagctc
     3721 caagtaagag ttttaactta gctggtatgc aattatcaaa tatagtaatc aataataagg
     3781 aattacaaaa aaaatggatg cttgaaacag attctaaatt agctatattc ctacctaatc
     3841 cattcgcaat agttgctact aaagttgctt ataatgattg tgaagattgg atgaatcaag
     3901 taaatgagta tatagatggt aatattaaat atgtagaaga ttttgttaat gagtatatgc
     3961 ctaaagtaaa gataataaaa tctcaaggaa cttacttagt ttggttagac tttagagggt
     4021 atggattaga tgcaaaacag cttgaatatg taatgcttaa caaggcgaag gtattgctag
     4081 atgaaggata catattcgga gaagaaggag caggatttga aagaataaat gttgcttcac
     4141 ctagaacaat tatagaagag tgtatgaata gaataaaatg tgcatttgaa ggattataat
     4201 agtaatatct aaataatata tttgtgtatt ttaaaaaaag actatgctaa aaaaatattt
     4261 caaagcatag tcttttaaag ttttatgatg agtttaaaaa atttcaaaac gttactattc
     4321 acttatatgt ttatattaga aataattcca caatatactc attttctaaa gttgcacttt
     4381 tatacacctt gcttcaccaa tatcatgtgt aaccaaaata gtagacttat tctcattctt
     4441 aataatacta taaacatcat cacaaactaa taatctagtc tgataatcaa gagctgaaaa
     4501 aggttcatct aaaagtagaa tatctggatt tacagaaagt gttctaataa gcgcaactct
     4561 ttgtctcata cctccagata attcttttgg atacatactt ctaaaatccc ataatccata
     4621 agtctttaaa aggctttcaa ctctatctaa agatttttta tcttttttgc cttgaatttc
     4681 caatccaata gttatattat ccattatatt tctccattca agcaaattat ctttttgaaa
     4741 catatatcca atatttccag taatctttat atcaccacta gtcggtttta aaagattagt
     4801 aagaatattt aatattgtag atttaccact tccagaagga ccaagcaaag ttaatatttc
     4861 tccttctttc aagttaaaat tgacatcctt taatacttca agctcgcctt ccttagtgta
     4921 gaagttcata tttatatttt taacttctac tatgttgtca ttcattatgc caccccctat
     4981 aacttttagc tcgtatatat tatgttatta acaatatgac ctatttgtac catttttcat
     5041 aaatataata taaatatttt gtttatttac gtattttctt agtaacttgt acgtataaat
     5101 tatagataaa taaattataa ggtaggggat aaatttgagt aacataataa cgaatttaat
     5161 agagtcaata tttactaatc caataactat attgttttta cttgcaataa ctgtgtattc
     5221 tgtttttaaa ataatagaaa ataacagagt atattcattt gtaagttcaa aattagacga
     5281 aatcaataga gactataaga caagtgagtt ttataaaaag gtaagggatg actactatac
     5341 atatagtaaa gaaaatcctt atgcagacgt aaatataact tcatttatag aggaagttgt
     5401 atctgattta aagcataata atttgccatt gttagaaaaa attagaagta taaaaaattc
     5461 atcttcaata agtatattac ttggggtttt aggtacgttt gttggtcttt ctacaatgct
     5521 tttatgtgtt gatactaaag acattataaa ttcacttcct tcaactatta gtagtatgca
     5581 aactgcattt acaactagta tatttggagt tgtattttct ctaattatag gttactttac
     5641 taagataaaa gattgtgaac atgttttgat acaaataatg ttaaaatctg aaaatttact
     5701 aacatctgaa ataactcatt ttaagtcaga aagaatggat ttaaaagtag aagaggtaaa
     5761 aaatacaata aaacaaataa gtaagtctat agaagctatt gaaaaatttg ataaaatatc
     5821 taaagattta aatgacttta atgatgaatt tatcagtggt atagaagctt taaaaagcct
     5881 tttagaaggt tctcaaagtt ctattaagac atttgaccag agcgtaagaa aattagataa
     5941 acagtttaat attttaaata taaaatttgt aaagcttttt gataaatatg ataatcaaga
     6001 taatataaat aaagagattt tatttgatat aaaagaatct tcaaaaaata tatacaatgc
     6061 cactgaaagc caattcaaaa ttagagatta tataaaaaat ataaatgcag gatttgctct
     6121 atatgaaaga agtgctcaag atttattaac taaattaatg acccatgaaa ataaaatatc
     6181 tcaaaatcaa aaaatccttt tagatgaaaa gtttacctta gatgactcaa taaaaaatct
     6241 ctcaagtatt attgaaaact tttctaatga cctacaagtt aaactggata tgatgtttga
     6301 aaattcttta gatattcaag ataagttaga tgttatgttt aataactctt ttatgaatga
     6361 tgaagttcct cttgatagtg aagaattatt taaggatgat gttaataatg tatttaatcc
     6421 ttttagtgaa gaaatttatg agatagagga taaagaaata aaggtcatag gtgaggatga
     6481 gttaaatgaa taataaatat agaagaactg ttaacagaga atttgaaaag agtagctttt
     6541 ggccaacatt tacagattta ttatctactg tattgatggt tgtgattctt atcttattta
     6601 gctcagaaag tatttcaggt tctgttgaac aagatttagc taagaatgtc aatgcttctg
     6661 ttgaagaaac ttttaaaaaa agtggaatac ctgtgaaggt agataaatct aatgggcaag
     6721 taacttttgg tgaaaaaacc atgtttgatg tagatagtga tgttttaaaa ccagaggcta
     6781 aagaaatgct aaaaatgttt gttccaaagt acatagagac tatttataaa gattatggtg
     6841 actatatatc caaaatagtt atagaaggtc atacagatga tgtgggaagt tatatataca
     6901 acttagattt atcgcaaaga agagcatata gcgttgctaa atttatagtg ggagatgaaa
     6961 taggtgacta taaatacaaa gataaagtga ctaagcatat tattgcaata ggaaggtcaa
     7021 aagctgaact catcaaaaat ggtgacaata gcgtgaatag agatgcatct agaagagtag
     7081 aactaaaata cgaaataaat attaatcaaa ataaataatt aaatttttat aaaaaaaaag
     7141 aaggcagttg gtgtgagctt taagccttct ttaaggaaaa agtttattta tttttgttga
     7201 tacttaacat attatagttt ttagaagcag tttgcaatat attaaacgta aatggtcgtt
     7261 taaaatgcat aactggatac tactgatgta aaatatttca ttttatataa tataaaaatt
     7321 atacagtgtc aattttctat attattgtac tataatctat tatttaaaat attatgtaaa
     7381 aaaatatcat aaaaacgcat atcatgttat aattactaga gtattatgaa tatggaggcg
     7441 t
//
LOCUS       DAYAOX010000183         7439 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.184, whole genome shotgun sequence.
ACCESSION   DAYAOX010000183 DAYAOX010000000
VERSION     DAYAOX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.184"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            65..1678
                     /locus_tag="ACGI57_002947"
     CDS             65..1678
                     /locus_tag="ACGI57_002947"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFG9718011.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            1953..3938
                     /locus_tag="ACGI57_002948"
     CDS             1953..3938
                     /locus_tag="ACGI57_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9718012.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            3943..4377
                     /locus_tag="ACGI57_002949"
     CDS             3943..4377
                     /locus_tag="ACGI57_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFG9718013.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKNLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            4406..4966
                     /locus_tag="ACGI57_002950"
     CDS             4406..4966
                     /locus_tag="ACGI57_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFG9718014.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            5030..5530
                     /locus_tag="ACGI57_002951"
                     /pseudo
     CDS             5030..5530
                     /locus_tag="ACGI57_002951"
                     /inference="COORDINATES: protein motif:HMM:NF015569.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
     gene            5570..5989
                     /locus_tag="ACGI57_002952"
     CDS             5570..5989
                     /locus_tag="ACGI57_002952"
                     /inference="COORDINATES: protein motif:HMM:NF019286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HFG9718015.1"
                     /translation="MAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFP
                     LLSPFLGPGAVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAE
                     AEPDTVQVGVPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            6030..6395
                     /locus_tag="ACGI57_002953"
     CDS             6030..6395
                     /locus_tag="ACGI57_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFG9718016.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            6496..7269
                     /gene="srlD"
                     /locus_tag="ACGI57_002954"
     CDS             6496..7269
                     /gene="srlD"
                     /locus_tag="ACGI57_002954"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFG9718017.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
BASE COUNT         2994 a          757 c         1432 g         2256 t
ORIGIN      
        1 gaatatccta aaaaagtata taatggatag gttaagatta attgataacg gagaggaata
       61 atatatgaat ataacaaagt ttgaagattt accaataagt gagggaataa aaaaagctat
      121 agctgaaatg gggtttgaag aaccatctcc tatacaagca cagtctatac ctgcgatttt
      181 gtcaggtaaa gacgttatag gacaagctca aactggtacg ggaaaaacag ctgcttttag
      241 tatccctata ctagaaacaa tagacccaaa taatagaagt ttacaagctg tagtgctttg
      301 tccaacaaga gaactggcaa tacaagtatc aacagaaata agaaagttag ctaagtattc
      361 gcacggaata aagacattac ctatatatgg tggtcagcca atagatagac aaataaaatc
      421 tctaaagagt ggtgtgcaag ttgtaatagg tacaccagga cgtacaatag accatatcaa
      481 tcgtaaaaca ttaaagatgg ataatgtaaa aatgattata ttagatgaag cagatgaaat
      541 gctagatatg ggatttagag aagatataga aatgatttta agtaagattc cagaagaaag
      601 acaaacaact ttcttttcag ctactatgcc aagaggaatt ttggaattaa ctaagagata
      661 tcaaaaagac ccagagcata taaaagttgt tagaaaagaa cttacagttt caaatacaaa
      721 gcaatactat atagaaacta ggtcatcaaa taaattagaa gtattatgta gattagtaga
      781 tgtatatgac cctaaactat ctgttgtttt ctgtaatact aagagaaaag cagacgaatt
      841 ggtaggagat ttacaagcaa gaggatattt tgcagatgca ttacatggtg acttaaaaca
      901 gactcaaaga gatatagtaa tggataagtt tagaaatggt acaatagata tacttgttgc
      961 tactgatgtt gcagctagag gtatagatgt tgatgatgtt gaatgtgtat tcaactatga
     1021 cttaccacaa gatgaagaat attacgttca tagaattggt agaactggtc gtgctggtag
     1081 agaaggtatg tcatttactt ttgtatttgg aaaagaaatg agaaagatga aagatataga
     1141 aagatatact aaatctaaac taataaagca taatatacca acaataactg atgttgaaga
     1201 gaaaaaagta ggtactttct ttgcacaagt taaacaaact atagaagaag gtcacttaac
     1261 taaacaatta caatggttag aaggtttctg taatgatgaa gattatgcaa tggttgatat
     1321 agctgctgca cttgtaaaac tatctctagg tgaagaaatg aaagaagaaa taatagaaga
     1381 aaaacctaga agagagcgtg gagatagaaa aggtggtact ggagctaaag atggtatgat
     1441 tagattattt atcaatattg gtagaaatca aagagtacaa gcaaaagata tagttggtgc
     1501 catagctgga gaagttggaa taccaggtaa agtagtaggt actatagata tatatgataa
     1561 gtatactttt gttgaaatac ctaaaaaaga tgcaaaaaca gtaattgaaa agatgaaaga
     1621 tattaagata aaaggtaata agattaatat agaaaaagca aataagaaga aaaaataacg
     1681 tatagtatac aaaactataa ttagaaaata tataaaataa tatattggat atttaaaaag
     1741 cctgatgtgt tttcaaaaca gatagtagat aatctgtaag agttaacagc ataaggcttt
     1801 tcttgtttta tatgatgtta taattttcca gattgttgtg aaaaatgtat attagttcct
     1861 ttttttcaaa tctgatttga aaaagagaat attaattatc tatatagttt agattataat
     1921 aaaaaatgta agataacaaa ggggagaaaa agatgtcaat gacttatatg gaccctaggg
     1981 tttttttgat tttagaaatg gtgttagaag aagacacaaa tactctagaa caattttcaa
     2041 atgttttggg tgttagtact aggactatca gaaactatat aaaccagatt aatcgagaat
     2101 taaaaggtgg tattgctaaa atagttaaaa atgaaaaagg tacatatagt ataagaattg
     2161 aggatgaaag taaattaatt gagattgtaa attttaatag aggaaagcta tcaaatttta
     2221 taaatttaaa ttcacctgat gaaagaataa attatgttct tgatatttta actatgacca
     2281 acaatccaat taccatagat gatttggctg aagagatatg tataggaaga acaacattaa
     2341 taaaggattt aaaaaaagca gacagaattt tagctgaata tgatttagaa ttaaaaagga
     2401 agccaaatat aggtatgatt ttagttggtg aagaaatgga tataagatta ttaatccttg
     2461 atagattgta tgaaaattat attgatgtct tggaaaacgt taatcagata aatttagtta
     2521 aagattatga tattgaatgt cttagagatg aattaaaaaa gttatttaaa aaagaagaac
     2581 tctacataac agaacaggtc ttgagagatg ttgaaagata tataatcatg tctgtcttaa
     2641 gaaatctaaa tgggtacaaa tttgaaaaga tagataagag atttgaagtg ataagatgtt
     2701 cagatgaata tactttagga ttaaagctca aagctttatt ggaaaagata tttaatatag
     2761 ttttaaatga aaaagaaacc atatttttga caatgccatt aataggtaga aaagctccta
     2821 gtacaaaatt agcacttagc agtataaaga taaatgatag cgtaaaggaa gttgtagatg
     2881 aagtaactag ttttttatta gagacctcag ggattgattt taaagaagat aaaaagctta
     2941 tagaaaattt ggaatatcac ttgtattttg cacttaatag aatgagattc aacataaggg
     3001 taaaaaatcc tcttttacaa gaaataaagg aaagatatac atttccatat agtgttgcaa
     3061 agatagctgc aatgataatt gaggaaaagt ttgacttgaa aatatgtgac cacgaaatag
     3121 gatatatagc attacatttt ggaagttatt ttgaaaaaaa ttcaagaaaa gttctaaatg
     3181 taaatagggt agctgttgtt tgtggaacag gtcttgggac tgcacaactt attagaataa
     3241 agataggaaa aatacttgga gaaagtgtag aagtaaatac tttttctgat atagaagcta
     3301 aagctaattt agatttatta aagtattatg atttagtatt tacaacagta gatttcaaaa
     3361 cagacttaga tgttccaatt tttagagtaa atgcgttatt taatgaagaa agtataaaga
     3421 aagagataga aaatgcaata cttttgaaaa atagtataaa tcttaagtct ataaaaaaga
     3481 ttgaaaaaaa tatccctttt ataggtaaat taattgaaga ggataagttc tttatattga
     3541 ataagagtac gtttatggaa aatttagaag atatgttaga aaaattagtt cataaaggaa
     3601 ttattgatat agagttcaaa aaaaaggtat taaaaagaga aaagaaatct tcaacagcat
     3661 tagataatta tatagcattg cctcactcag ttaatactaa tggggaaaat ctttatctgg
     3721 catttggaat attagaaaaa ccagtgattt gggatacaaa ggaaattaga ataataatat
     3781 tgatgataat tccagataag aatgtagata gtacagacct gataataaaa agttatgaag
     3841 aagtacttga agtagggaga gataaaaaga tggtagaaga acttgctaag gcaaaatcat
     3901 ttgatgaatt ttataaaata attaccaaga ggaggtaata atatggaccc agtaattttt
     3961 attatagcat cacttattac agtgtattta ttaaatattt ttcttggata cttacagctt
     4021 aaagatttta ataaaaatta tattgaacta aaaagaaaag gaagagtggc aattggcaga
     4081 aaaaaaggaa gaatttcatc gggtacaatt gtgttgatac ttataaatga tgatggtgtg
     4141 attgtcgaga ctagaaaaat gcaaggtgtt acagtattgg caagagtaaa gtcatttgaa
     4201 ggtttggtag gaaagaactt agagtatata gaaaaatcag atttaactgt ttacaataaa
     4261 ctacttaaaa aagcaatact agatgcagtc aaacagtaca taacatttga gaaaaataaa
     4321 tgtgaagata caaataatct gagtactgat gaagagtgta ggatggaagt agtttaaaaa
     4381 aatatattta taaggaggag ttaatatgac gcaatttttt caagtattag caagtggtgc
     4441 agatagtttt atgaatttat ttagggcagg tgggcaacaa tttgtaggtt ttgtaacaga
     4501 tatagtacca cttttagtag cattattagt tacaatgaat gcaattatca actttattgg
     4561 tacagataga gtcgagagac ttgcaaaaaa atgttctgca aatatattta ctagatattt
     4621 gatattacca gtccttggta catttgtatt tgcaaaccca atgacactat cattaggaag
     4681 attcttgcca gaaaagttta aacctagtta ctttgcagca gcaagctttt catgccatac
     4741 aatgaatgga ttattcccac atatcaatcc aggtgaacta tttatatatc taggaattgc
     4801 aaatggtata acaactttgg gattctcaac agcagattta gcagttagat atttgttagt
     4861 gggtatagtt gcaaacttta ttaaaggtgt agttactgac tatacaacaa aatttgtaga
     4921 gaaacaacaa ggaatagcat taaattcaga tataaagatt ggataattac tttaaggtat
     4981 aaataaattt ttcaaataaa aagatagtgt atagaggagg ataaattata tggcatttaa
     5041 caaagttaaa atagtaaaag gttctggagg atggggtgga ccattgataa tagaaccaac
     5101 agaaaagaag aataaggtag tatatgtgac tgggggagca aagccagaaa ctgcagtaaa
     5161 aatagctgaa cttacaggat gtgagttaat agatggattt tcacagggtg ttaaagatga
     5221 tgaaatcgca tgtgtaataa taaattgtgg tggaacattg agatgtggta tatatcctca
     5281 aaagaaaata ccgactataa atataatgaa aacaggaaaa agtggaccat tagcaatgtt
     5341 tataaaagaa gatatatatg tttcaggggt aaaaccaaca aatgtagagc agttataatt
     5401 gaggtagtag gagggaagaa atatggctta tgatactaaa aagaagataa gtgagcagag
     5461 tcaaggaatt gttgcaaaaa taggtatagc actaggaaat gtgacaagta ctctttttca
     5521 agctggtagg gatactatag atacattaat acatactata ctgcctttta tggctttcgt
     5581 tgcagtccta ataggtataa taaatcaatc aggtatagga aattgggtag ccaatttact
     5641 tactccatta atcggaaatg ttggaggttt agttgtctta tcaataatat gttcatttcc
     5701 gttgttgtct ccgtttcttg gaccaggagc tgttatagct cagatagtgg gagtattggt
     5761 tggtacagaa ataggaaaag gaaatattcc accaagttta gcattaccag cattgtttgc
     5821 aataaactca caagcagctt gtgactttat accagtaggt cttggtcttg cagaagctga
     5881 acctgataca gttcaagtag gagttccgtc agttttatat tctagatttt taacaggtgc
     5941 accaactgtt ctattagcat ggttagcaag ctttgggtta tataaataga agaatacttt
     6001 ataaataatt agagagaggt tcagttgata tggaattaat atacagtact caaataaata
     6061 aaataggaga taatgcaaat gaatttttac aacataatat gtttgtaact ttcaaagata
     6121 atgctcctca agagttagaa aattactgtt atatacattc tgaaaataat ttagtaaaag
     6181 aaatacttac ggatgatgtg ctatcaataa ataatgttga ttacaaaatt acttctgttg
     6241 gtgagttagt aaaccaaaat ctttcagaat taggtcatat aacatttaag tttacaggtg
     6301 aaaaagatgg agcaattgga ggaaccttat atcttgaaga aaaagaaata gcacctattg
     6361 gcgtaggtac tatactaaaa ataattagaa aataaaattt aaaacgaatc taatcatatt
     6421 ataagtaaat tatagataaa agtcaattta actaatcaag ttaactttta atgtgataaa
     6481 aaaggggaaa tgaaaatgag aaaagtggct ttagtcgttg gaggaggaag aagtttaggt
     6541 gaatatttat ctaaacattt agctaaagta ggttatgatg ttgctattgc agatttaaat
     6601 gaagacaacg caaaaaaagt agctgaagaa ataaaaactg aatttggttg taaatctgta
     6661 ggatatagtt gtaatgccat agatgaaaga caggttataa gtactgtcaa agctattgaa
     6721 aatgattttg atagaataga tttattagtg tacaatgcag gagtggctgt aagtaaaaaa
     6781 ataactgatt ttggatacaa agattttaaa tttattgtag acgtaaattt aaatgggtac
     6841 tttctatttg caagagaagt atctaaagtc atgatagcaa aaggtattga gggtagaata
     6901 atacagataa attcaaaatc aggtagagtg ggaagcaagc ataattcagg atattcagca
     6961 ggtaaatttg ctggagtagg acttactcaa agtttagcgc tagatttggc agaacataag
     7021 ataactgtaa atgcactgat gctcggtaat ttacttgatt ctgatatgtt tgaaagttta
     7081 attcctcaat atgcagtaaa attgggaata cctgaatcag aagtcaagca agtttatata
     7141 gataaagtac ctttaaaaag agggtgtaga tttatagatg ttgcaaatat gcttacattt
     7201 tatgcttcaa aggaagctga atactgcact ggtcaatcta taaatattac tggtggacaa
     7261 gttatgtaag gtttttatgg agtgtctcaa gatgaactct ttagttcatg aggcactttt
     7321 ttgtataaaa ttaaatatat tttcagcatt taaacaatga aaaagaggtt tatctttatt
     7381 ttgagatagc ctcatttttg taattaaaaa acatagtgtt taagtaaaca tataagatt
//
LOCUS       DAYAOX010000184         7394 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.185, whole genome shotgun sequence.
ACCESSION   DAYAOX010000184 DAYAOX010000000
VERSION     DAYAOX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.185"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(248..2122)
                     /locus_tag="ACGI57_002955"
     CDS             complement(248..2122)
                     /locus_tag="ACGI57_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFG9718018.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLASIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(2127..3791)
                     /locus_tag="ACGI57_002956"
     CDS             complement(2127..3791)
                     /locus_tag="ACGI57_002956"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFG9718019.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKKSKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(3805..4626)
                     /locus_tag="ACGI57_002957"
     CDS             complement(3805..4626)
                     /locus_tag="ACGI57_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFG9718020.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(4725..5489)
                     /locus_tag="ACGI57_002958"
     CDS             complement(4725..5489)
                     /locus_tag="ACGI57_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9718021.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVINDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(5486..6235)
                     /locus_tag="ACGI57_002959"
     CDS             complement(5486..6235)
                     /locus_tag="ACGI57_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9718022.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(6739..>7394)
                     /locus_tag="ACGI57_002960"
     CDS             complement(6739..>7394)
                     /locus_tag="ACGI57_002960"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9718023.1"
                     /translation="LLINYIGKIPNKGNLFMKIKENVLMLDSTRGSNCFIVFDKEIIL
                     IDTGLPFMGKNIIKELKTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVE
                     DIPFIIGEKDRPGFKKFIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMI
                     FEDVLFAGDLVKNKNGNIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
BASE COUNT         2515 a         1319 c          752 g         2808 t
ORIGIN      
        1 taaaaacata atataatacc ctatacaaat agtggcaact ctccctcaca tcctcaattg
       61 ttgccactat ttaatttttt gtatgaagtt aaatatattt tcatcatttc aacaatttgg
      121 gtgcaataca atctctattt tgaaacaacc tctttaattg ttttagagtt tatgaattat
      181 ttctcatact ttaaaaagat tgaattactc ttgataatct tcttttgtag tcattacata
      241 tcacctacta ctcaacacat attctttgac ctatttttac atccttgttt atatccaaaa
      301 tatttacctt cttaccatct ggaaacaata ccattgttaa agtagacttt cctaattcat
      361 gtattctctt taaatcaaca ttaacaagaa catctccctg tttaaccata tctccttgtt
      421 taacagcaat atcaaagcca tctccattta atgcaactgt atcaattcca acatgaatta
      481 atacttctcc attagttcct ttaatcccaa atgcatgtcc acttgaaacc actgtttcta
      541 atttacctga aataggagaa acaatttttc catctataat atcaatagca aaaccatcac
      601 ctaacatttt tttagaaaat gtttcatctg gtacattttc taatgcaaca attttcccct
      661 tagctggaga cacaaagttt tcattatttt cattaaacac taattcttcg tcttcatctt
      721 catttatagc ttctttaggc actccaaata aacatgttac aataaagcag aatgcaacca
      781 caaatattga tgctaataag tatgtgatta aattatatcc atcatagata tacattaaat
      841 aagaaggtaa aactgctaat ccatatgaat tagattgaat atgtaaaatt gataatattg
      901 ctgctcctaa acctgaagat gcacacataa taatcaaagg ctttaaatta tatcgaagct
      961 gaatcccaaa taaaacaggc tcacttatac caaaaagttg agatagcatt gctccaactg
     1021 cagtttgttt aacttgatta tttttagatt ttttcataaa tgctagacat gttccaatat
     1081 tcgcaaatcc atacattgca caaagagtaa taagggcatt aaatcctgta tttgctaata
     1141 gtgatgtttc aatcattaca taagtatgat gtaagccagt aataacagct aatggatatg
     1201 taaatccaat aataaatcct ccaattccta aaggcaaatc aatcaataag ctgatgatat
     1261 caaccatttt taattctatt gtatgcatta tagggcctac acctaacaaa ataactaaca
     1321 ttgttattag cataacaaaa aatggtgtga aaataaggtc taatacatta ggcatatgtt
     1381 tacggaacca tttttcaaga ttagctccaa taaatgctgt aacaatagct gttaaaacac
     1441 ttccttgaca acctacaata ggaataaatc caaatgccat aattgcttca atttcatttg
     1501 atgggtcagc aacagaatat gcattaggaa gtattgggga aactaacata agtccaataa
     1561 caagtccaat aacaggtgtt cctccaaata ctttaaatgc tgaccataca ataattgctg
     1621 gtaaaaaagc aaatgctgtt tctgtaagaa ccgaaacaag tgtctgaata tacaatggaa
     1681 tatttgcaga agacattcca aataatccaa gtacattatc attaaacaaa caacccttta
     1741 atcccaagaa taatcctgtt gctgcaataa ctggaataat aggaacaaat atatctgcta
     1801 gagttctcat aactttctta aatccagttt cattattttt aactaattca tcttgttctt
     1861 tttttgataa agtttttaat cccatttttt caacttctgc ataaacttta ttaactattc
     1921 ctgttcctaa aataatttga tactgtccag aagtaaagaa aaccccttta acctcatcaa
     1981 ctttttcaac tttcttatca ttaataattt ctctatcttt aaccatcact cttaatcttg
     2041 tggcacaatg tgtaatagaa ataatattat cttcaccaat aattttaata agctcatttg
     2101 ctattttatt atatttattc atttttctac ctcactttca ataatactgc atgaaatggt
     2161 ttcaaacgca ctgttttatt taatagattt atatcatcta aattttggaa tacaatatca
     2221 tatttttctt ctactcttat ttctacaggt ttatcagaaa aattataata acaatcaata
     2281 actaccaaat ctaattgcct tctatacgca ataatatcat cactttctat atcaataggt
     2341 tctattgaac cataaatcaa acaatcagaa tactcactat cttgtctcaa ctttatcatc
     2401 tttttataaa attctaaaat actctctgaa ttgttaatct gactctgtgc attaatatct
     2461 ttataatttt caataacctt taaccatggt gttgtatctg aaaaacctgc attttcttta
     2521 tctgcccatg gaaatggcgt tcttgcatta tctcggcttc ttaaattcac aaattctaac
     2581 gcttcttctt ctgagtaacc ttcatctatt gaacgatagt attggtcaat gctagataaa
     2641 tcattaaaat catttatggt atctctttta aaatttgtca ttcctaattc ctgtccctga
     2701 taaataaatg gggttcctct taaaaagaaa tacattgctc ccaacatttt tactgcatca
     2761 tcattccttt cgtattgcaa aagatacttt gttgtagctc ttggttggtc atgattttca
     2821 ataaaattag ctgcccatcc atgttcctga acatacattt gactatctaa aatgcatttt
     2881 cttaaatctg gaattgtcca tgaaattctt ttaaaccact cactaccaga agcaacatct
     2941 aagtcagcat gtttaaagtc aaagaccatt gaaaagaaac catcttctcc aataaaatca
     3001 cctaactggt catagctaat acctgctgct tcagcaacag tcatacaatc atatttttta
     3061 aacgtatttt cttttaattc ataaagcatt ttttctattc caggctgatt tctaactgtt
     3121 tttgtacatt taacaagacc atcaacacca tcactttcta aactttgaaa acttaaattc
     3181 tttttgatga atgtaatagc atcaactcta aatcctgcca ctcccatttg agaccaggta
     3241 ttaatcattt ggtaaagttc acttcttaat attggatttt cccaattaag gtcaggttgt
     3301 ttttttccaa aagaatgaaa ataatattcg tctctattct ctaccttttc ccaaacactt
     3361 ccaccaaaaa cagacctcca gttatttggc aaatcgtttc cttttttaaa aatataataa
     3421 tcatgataag gactttctgg attttttaat gcttcttgaa accaaggatg ttcatcactt
     3481 gtatgattaa gaactaaatc taatataatt ttgattcctc tatctttact ttttttaatt
     3541 aactctttta aatcttccat acttccaaat tcacttgcaa tatccaaata atcactaata
     3601 tcatacccat tatcatccat aggtgattta aaaattggac ataaccacaa tgatgtaata
     3661 cctaaatctt ttaaataatc taaacgagaa ataattccct gtaaatctcc aattccatca
     3721 ttattactat cttgaaagct tcttggataa atttgataaa cgatttcttt ttgccaccat
     3781 ttcttttcca taaattactc ctttttatat gatatttctt ctttttaatt ctttataaat
     3841 tccattatcg aatatgtcct cacatataga tgtcgctata tctttaagtt gttgatgact
     3901 atttttcatt gcaattgtaa cgtctgatgc ttgaaacatg acaatgtcat tttgaccatc
     3961 accaaaacat atggtttctt tttgagttac tcctaatctt tcttgtaatc tcttaattgc
     4021 tttgccctta tgaaaatctt tttgaataat ttcataatac tgactagaaa tatctctctg
     4081 tgctaattcc attttatctt gtagtatatc tttcacttcg tcaaatacct tttcatttga
     4141 ccataaacaa attttatgta tatcttgtga cttatactct tcaatgttat tttcatatgt
     4201 tattttttct tggatatgtt gtttatttat acaagaatta gttcctttca actgattcat
     4261 tgtttcaaat atttctttgg ctttttgatt catgaaaact ttttcctgac tctcaatcga
     4321 aaaagcaact tctctatttt ttaataaaca aacaacttcc tttatcaatc tttgattaaa
     4381 agattgatta taaagtaatt caccatgata ttgaatgtaa ttacctcctc ctgcaatata
     4441 tccatcaact cctaatgata aaacatcatc ttgtattgta cccatacttc ttccagtaca
     4501 aataacaaca ctgcaatgat tcttttgaca taaacgtata gcatgctttg cagattctgg
     4561 gattccatat acctcatctc taagcgtacc atctatatca agtattaata atttgtacaa
     4621 tttcatcttc ctttcttact atcaaaatca taatagcatt caaaaaaaaa gatatcaata
     4681 ttttgatatc ttttaaaaaa tatgttcatt tttagcacaa attttcattt tcggggaata
     4741 tgttcacttt ctaaatacat cttgtattta atataaagca gttcaattaa aataaaataa
     4801 ggtgttgtta catcgtagtc cagttgttta tctacagaaa tagatataga ttgaatatac
     4861 aggttttcat cacataatct tgctaaagga ttacttatca tttttgtaat agaaaccata
     4921 taaattcctt tcattctcat ttctttagca aaatcaataa ttgtagatgc atctccattc
     4981 aatgaaataa agaaaatcat atcatctgtt tttataaaat gattaagtgc ctgaatcata
     5041 tcataaccat aaacatcata tataattttc cctgttggta agaaaatcct tttcatctct
     5101 ttagcaactc tttcctgaga ataaccttct ccaaagacga ttatacgatt tgcctttttc
     5161 attctttcaa acataatcga acaatccttc ttctctatat catttatcac cttattatag
     5221 caatccatta attggtgttt actttgattt ttatattcta cttctgtttc atttaagcgt
     5281 ataataactc ttaattcact atatccaggt aattgtagtt tttgagcaaa acgagaaagt
     5341 gcacttgtag atatatggta tttgtctgca aattcatcta tagaaagttt aatacaatct
     5401 tctttatgat taagaataca ctcacagata tacttatcat ttgctgtaaa tgaattataa
     5461 tatttattga aaagtccttc aattttcatt cttttctcct tctatactct atgtaattca
     5521 tatgaagcaa atctaataga gcataaaatg ctcctacgac ttcataagat atagcttgta
     5581 atgtatcaag tttttgagtt tcaacataca gattatactc acaaagtgtt gcaattgtat
     5641 tattatcaag cctagttaaa gaaatcgtat gtatatgatt gttgataaat tttgcaattt
     5701 caattcctgc ttttgtttct ccagacaaag aaatgataat aaataaatca cttttttgaa
     5761 atgattctct cataaattca atttcaccta tatcaaataa gtcaataaca catttaccta
     5821 ctgaaagaaa tattctcttt atttcttgag ctaaactttt ctgttcatta cctgtcccat
     5881 aaatataaat tgtttctgca tcatatatta ttttacaaat atcttgaaat gatatttttt
     5941 gtaattcatt cattaatagt cgataattct cgcaaacatc atgaaaatta ttatcattat
     6001 taacaaattc tagttgatta tttttaagta aatactttaa atcagagaat gaatctaatc
     6061 caatttttct acaaagcctt aataaagtag ctctagaaac attacattca tttgcaattt
     6121 tttcacacga aaaattacgg tattctcttt gacaaatcaa tatttgttta ataataattt
     6181 tatcattttc atttaaatta tcataatttt ggttaactaa cgattctaat ctcatttttt
     6241 ccactcccta aatattattt taatatacat tagttgcttt ttctagggaa gagttttaaa
     6301 taataaggat aaaataatca tttgatttta tttttattaa aataaaaata acctgctaaa
     6361 agcaggcacc aaaaagtact tatcctttcg aacatgtact ttagatattg ctttcttcgg
     6421 aagcttcatc tcttcatact ctaaaataga gttttttcta actcaaaagt attttccatg
     6481 gtagaaatta ctttaattca tctgccattt attttttatc ttatttaata attactacta
     6541 cttgaatatt tacttcttta agtaaatttc atagttaaca aagctaaaat tattattata
     6601 gatgttaaaa tacaaaattg taagtattgt tataaatatg cttacagcta caaatataca
     6661 tgatatatta aaaagaagct gtaaattatt atcatacata caagcaacta cgcctctatt
     6721 aatccatgtt aatttttatt atttaataca aggagttcca tgagacatac atatccattc
     6781 atattttaaa ttatccaatt ctcttataga tttcatcatc tttttataat cccaattcca
     6841 tggattagga taaggaacta tatttccatt cttatttttg actaaatcac ctgcaaataa
     6901 tacatcttca aaaatcatgc agacatgccc tggagtatga ccaggagtat ggataatttt
     6961 tatattccca atctgcatat tctctccata aggttcaaca tccttaatga gccttctgga
     7021 aattgccttt ccaataaact tcttaaaccc aggtctatcc ttctctccta taataaaagg
     7081 tatatcctct acatgagccc atacttttgc tcctgttttt tctttcaata atttaatatt
     7141 agatatatgg tcaacatcat gatgagttaa taaaatatgc ttaatatctg taagtttaat
     7201 gccaagtgtt tttaattctt taattatatt tttccccata aaaggaagac ctgtgtctat
     7261 aagaataatt tctttatcaa acacaataaa acaatttgag ccacgagtac tatctaacat
     7321 taatacattt tcttttatct tcataaataa attcccctta tttggtattt tacctatata
     7381 attaattaat aaag
//
LOCUS       DAYAOX010000185         7339 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.186, whole genome shotgun sequence.
ACCESSION   DAYAOX010000185 DAYAOX010000000
VERSION     DAYAOX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.186"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(126..725)
                     /locus_tag="ACGI57_002961"
     CDS             complement(126..725)
                     /locus_tag="ACGI57_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viroplasmin family protein"
                     /protein_id="HFG9718024.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(743..3862)
                     /locus_tag="ACGI57_002962"
     CDS             complement(743..3862)
                     /locus_tag="ACGI57_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFG9718025.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANKIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINFIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            4083..4907
                     /gene="pdaA"
                     /locus_tag="ACGI57_002963"
     CDS             4083..4907
                     /gene="pdaA"
                     /locus_tag="ACGI57_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HFG9718026.1"
                     /translation="MKKDSLKKYIMIGAFALTLFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(5004..5789)
                     /locus_tag="ACGI57_002964"
     CDS             complement(5004..5789)
                     /locus_tag="ACGI57_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9718027.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEGGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5889..6023)
                     /locus_tag="ACGI57_002965"
     CDS             complement(5889..6023)
                     /locus_tag="ACGI57_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718028.1"
                     /translation="MNISRKAMKIIELAQKIANKRGISVEEAWSEAVTEYKNKYEHIA
                     "
     gene            6254..6619
                     /locus_tag="ACGI57_002966"
     CDS             6254..6619
                     /locus_tag="ACGI57_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9718029.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            6697..7251
                     /locus_tag="ACGI57_002967"
     CDS             6697..7251
                     /locus_tag="ACGI57_002967"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFG9718030.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYYK"
BASE COUNT         2540 a         1158 c          681 g         2960 t
ORIGIN      
        1 acttattttt tcataataca atctgctttt ttaatcaaaa tttatttaat tacaagttgt
       61 ttaattataa agtaggaatt tagatatact cttataccat gtaattatgt ataaatattt
      121 ttttattata caccaatagc ttttttagct aatttatcag cttcctcatt atacttattt
      181 ccagaatgag ctttaacctt tgtaaatttt acattaagct tattcttaat actgtcatag
      241 aaatttttat atgctatagt tccttcttta tttgttttcc aaactccagt acaccacttt
      301 tcaatccctt catagtcaaa gtataaaatc aagttttgaa cattattgtc aatacagtac
      361 tgcattgcaa ttttagaagc ctctatctct ccagctacat ttctcatact tactaaagtt
      421 ttctcttttc ctttttcact ataagttttt ataacttcat tattttttaa aatcacaaca
      481 cccgaaccat acatttttac tgaatgttca taacttccat ctacatatgc ttctataaac
      541 tccttatctt cttgaaaact caatttttct gacccatcaa tatattcttt agcttcttga
      601 aaagtagaaa aactcttata ttcagctcct gagaatccat ttacttgttt tttgcactca
      661 tcccatgtat tatatacacc tatattttta ccttttttca cagcataaaa cttttgtttt
      721 gccaaatata tcactcccac tcttaatttt tatcttcttc tctcataaat atatctaaat
      781 ttataagata gtttctatca aaatatttta ctttcatatc tttttttata atatcaatat
      841 ggtacacttc tccttttaca gctctttgtc ttatattttc atttatatct ttttttataa
      901 tatccttatc aacatcattg ttatccattt tagcatcaac attcaacgct tttatagtat
      961 caaatataac agattttagt attaaacttc tagatttatt taaatgtcgc cttatataag
     1021 tataaaactc attgctatta acctttgaaa ttttacaatc gtgtaaatct ttctctaatc
     1081 tctttaacac acttatatta gttcctacat caaaaatcac aaaacatcca tagtcatatt
     1141 tgcttcttaa aattctcccc atagcttgct ttacctttat ggtcatttga ggataattta
     1201 tagtatagta gtctatacca tacttcttca ttatagtaaa atacaaaggg tctttaggat
     1261 ttagattcgg aagcttgtct aaagttacac aaaccaaacc atcccctgga atgtctacgc
     1321 cttcaaaaca tccttttgag ccaagtacta cacattttct atttaaatcg ttaaggtgtc
     1381 taatgccctt tttatcagca taaatttcta agttaaaact atgtaagtat tttttcaata
     1441 tttcataagt tttttcttgt ctatctttac tattaaaaag agctaatgta tgaccttctg
     1501 taatctttga tatattagat ataattttac tcatctcatt tggaaattca gaatttctgt
     1561 actcacaaat atcagaaaat ccaactacag agactctatt tttataatca aaaattggtt
     1621 ctataatctt ttccacattc ttaactctgt ctattcctaa tgtattttta aaataagaca
     1681 tactttcaga taaactcaaa gttgctgata aaaatattcc tttttctaat tgagataaaa
     1741 tattttcttc aaataaatct gctattttta atggtactac tcgaaattca aagtcattat
     1801 aattcttatc aatttcaact atccttgcat aatcatcctt ttcatcatac tctaaaaaga
     1861 tttcaaatat aatctttata tcctctaaat ctcttgtctt tgcctttcca aatttatata
     1921 tatctgcttc tttatctata ctatcatcat caatattcct ataaattatt attattattg
     1981 aaactaaatt ttttattatt ttctcacaac tcaactttat cttttcacta taggctctat
     2041 aacttatttc agtatctata ccatcttttt ttagttttcc cacaatttca tctatttgca
     2101 aattcagttc ccatcttaaa ttataattac ttacattatt atattcatta caattaccaa
     2161 actttaatat tgaatctatc tcttcaataa tgaaatttat acttctagca attttttgtt
     2221 tatcttctct tccaattttt aatacattat aaaatttatc aaaagccttt atttttctca
     2281 tatttctact atatttttta tatataaagc tactattttg aataaactcg taaggataaa
     2341 tttcttgtaa taagtacctt aaagactttg aatttataat acttgaaaag aaatcatatc
     2401 ctttttcagt caaattatgt gcttcatcta ctattatatt ttctaaaggt ttttcatcct
     2461 tatacggcca tttagctaat agtgaatgat ttactactgt tatatgttct tcttttaatt
     2521 cttctattct gtttttatat aaacaatctt ttttgcattt tttaggtctg cacatatttg
     2581 ggtcacaact tacatttctt aagtgagtac ttatttcctt gaagttgtca aacactaaat
     2641 aatttatttc ttctatatct ccatacttgc caccttcaac taatctcttc aaaaatatta
     2701 aagataaaat ttctccttta gtagggtttt gagactcata atcatctata tatgcttcca
     2761 gtctatctat acaaatatag ttattttttc cctttatata tccgtaactt acttttccat
     2821 ttagacccaa agaatctaac acatttggaa tatctttatt tattaattgt atctgcaatt
     2881 cctttgtgtc agtagaaatt aataatcttt ttttattaat tctagcctct aatattgcag
     2941 gcaataaata tcctacactt ttacctatac cagttggtgc ctctatacat gcaatttttt
     3001 cttcatcttc gctatttcta aacaactctc ttattgtttt agtaagctca tattgaccag
     3061 gtctatattc atatgtaaat ccttctttat tctcccaaat agttttgtat tttaataatt
     3121 cttcataatt tttttcttct tcatttaaaa tttttaaggt ttctctttcc ttcttctttt
     3181 cttcttttga gtcaaatata ttatattctt tttttatatt tatattattt gataaatcat
     3241 aatttgcctc ttctaaaaat ttgctccatt cccatgttgg aagattaaat tttttaagat
     3301 atgaatttat tttaaaactc aatggctcca aagtagtttt ttcatagtta ttaaatttta
     3361 ctaaaaggca atttacaata tttattatat caatagcatc tgataaagct ctattttcca
     3421 ctttagaatt acaatttgtt aaagtatttt ttaagtattc taagctataa tctttatgat
     3481 atggttctaa aataactgct aattcaatta aatcaataaa tttatttttt aactttggca
     3541 tatgatactc aaaaaacttt tttttcagac ttaaatcatg acatataatg aaactatctt
     3601 ctacaaaatt tacaagtcta tcttccacca gttctatttt attagctatt tctaaatcat
     3661 ttttatctaa atttttacat actgagaata cttctatagg tacctcaaat ttgtttttta
     3721 ttaatgattc ataggtatat attttccagt ctttaacctt gacagctcca acttctaaaa
     3781 tttctgaatt taaacaatct aatccactaa cttctatatc taaaaaaaca acatcattca
     3841 atacacttat tatattatcc atttttatat cccttcaagt taaaccagcc taacttattt
     3901 acaatcattt ctctttatat tttatcattt atatacactt tatttaagat aattaatatc
     3961 aactttatat cttacagtaa acttctttca tttattattt gtatatgtga atatttttaa
     4021 ctataaatat tcaaactata ttataagaaa tatactttaa attttaaata agaggtgaaa
     4081 ttgtgaaaaa agatagccta aaaaaataca ttatgatagg agcatttgct ctaacacttt
     4141 ttggaattgc tagtataaat tttaaatcat tagataaaac aaaaactcaa atttctagtc
     4201 caacactcga cacacatgaa tatgattggt attttaaccc tagggaagat ggtaaacagc
     4261 catcacctat aaaagaagct gattttttta aaaaatacgg agcttattat gtaggaaatc
     4321 ccaatgaaaa agttatatat ctatcatttg atgctggata tgaaagtgga aacactccaa
     4381 aattattaga tacgcttaaa aaacacaatg ctaaagctca atttttcgta gttgaaagtt
     4441 atataaaaag caatccagag ttaataaagc gtatggaaaa agaaggacat ttagtttgta
     4501 atcattcaaa aagccatcca tcaatggcag gtattacaga ttttgaaaaa ttcaaagaag
     4561 aaattacaag tgtagaaaaa gcatacaaag atgttactgg aaaagaaatg cctaagtatt
     4621 ttagacctcc aatgggtaaa tttagcgaac aatctttaaa atatacacaa gatttaggat
     4681 ataaatcaat attttggagt tttgcttatg tagattggta tgaaaagaaa caacctactc
     4741 atgaatttgc caaaaacaaa atttattcaa gaactcatcc tggtgcaata gttttactgc
     4801 atccaaattc atcaacaaat actgagatat tagatgaggt tcttacacat tgggaaaaag
     4861 aaggttataa acttaaaaca cttgattatt taaataataa aaaatagtat cataaattta
     4921 aaaaaactct atcatgaatt atagtaaaaa tatagttatt tataaatact ttaattctcg
     4981 atagagtttt ttatttttta tagctattta aaaagaattg tccattcatt tatctctttg
     5041 tatcctatat tatgatatat tttacctgct acatcattat tataaaaaag acatggaact
     5101 tttccttcta acaacaattc tttactcaaa ttgtaaacca tataacttgc aagtcctttt
     5161 cccctatatc ttttatctgt acttacagag accaccattg ccaaaaaact agtttccata
     5221 ccagttgaca cagtgctaat catagtatca tcatttctaa taaaatatgc tcttacattt
     5281 ccctctttta aatgaacttt tctttcttct atgtaatttt tacttacttt atagtctgac
     5341 ctatttaaaa gttttcctat ttcatcaata tctttttcct gagcattttc tattttatac
     5401 tccttcatgt tggaaaaatc tatttctttt atttctttta atacacaaaa tttattatct
     5461 agtttttcat aaaaatcttt gtatttactt attagtctat ctattacaca tttttttcca
     5521 cttatgcttt ctacatctaa atcttttata tgattcttca tttctcctat attaaaatta
     5581 tttttaaaac tataaattat aagtgttgac ttatttttta atattataga ggttatattt
     5641 ccaccttcta cctgagacca tatatcttga tattgagagc taaatccata attctcaacg
     5701 tatccaatta tgaataaatt taaactttct tcatgtgata catattgcca aaattctttt
     5761 tctaaagtat tatctatttt tacaatcaag cagatatcct ccttctatgt taataatgat
     5821 ataccttata atttatagta tcatttaaaa tcattatatt ctaccaaata ttctaaattt
     5881 ttaaatatct atgcaatatg ttcgtattta tttttatatt ctgttactgc ttcactccaa
     5941 gcttcttcta ctgaaatacc tctcttattt gcaatttttt gtgctaattc tattattttc
     6001 atagcttttc ttgatatatt catatatatt cctcctctaa attatcataa gttattttaa
     6061 agttaaattt ctatagtcat gttatatatt tatttactta tatcttacta attatttatg
     6121 tactaaatat gaactacaat agaagaaatt tagaacttaa taaaacataa aatttatata
     6181 cagttcatat atatttgtca tatcacaaga caagtgatat aatttattat cattttactg
     6241 ggggtgatat gctatgaaaa taagagctga atatacatgt cctcttgaaa ttgtacatga
     6301 cataatcaaa ggaaaatgga aaactatcct tatatttgaa ttaagacaat ctaataaaac
     6361 attttctgaa ttgcaacata atatttatgg aataaatcaa aaaatgttga ttcaacaatt
     6421 aaaagagtta cgtgattttg ggattattga taaaataagt tctgctggat atccattaca
     6481 tgttgaatat tttcttacta atcgtggtaa aaaaatgtta aaagcagtgg aaataatgca
     6541 agaaatagga atcgaatata tgcttgaaca tggtcaacaa gaaattctag ataataaagg
     6601 aatttgctac aataagtaat cctatacata ccaaaaagta agtaatttac attttttaca
     6661 atagtattat aataaaaata aataggaggt ataatcatgt ctatagcttt aatattgatt
     6721 gatattcaaa atgattattt taaaaatgga aaatgtgaat tatttcaatc agaagaaact
     6781 gctgaaaatg ctaaaaaaat actactgttt tttagaaaaa acaatttacc agttattcat
     6841 atacagcata tatcaacaga taaaacagct tctttttttc ttcctaatac tcatggttca
     6901 gaaataaata aaattgtttt tccactagat aatgaagaaa ttataattaa acacacacca
     6961 gatagtttct ttgaaacaga tttgcaaagt atccttgaga aaaaaaatat tactaatcta
     7021 gtaatctgtg gaatgatgag ccatatgtgt attgatacaa gtgtaagaac agcaaagaaa
     7081 cttcgatacg acataactct aataagcgat gcatgtacaa cgaagaattt atcttggaat
     7141 aatatagaaa ttaatgcaaa tactgtacat caagtattca tggcatcact acagaacagt
     7201 tttgcagatg taaaaaacac agataaattt ttatctaact attacaaata aaaacataca
     7261 ctatgttaaa agagcaagtt tgatttattt aagcttgctc tttaatcatt taaaattaga
     7321 tgctttctat catttttat
//
LOCUS       DAYAOX010000186         7261 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.187, whole genome shotgun sequence.
ACCESSION   DAYAOX010000186 DAYAOX010000000
VERSION     DAYAOX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.187"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..490
                     /gene="bilQ"
                     /locus_tag="ACGI57_002968"
     CDS             <1..490
                     /gene="bilQ"
                     /locus_tag="ACGI57_002968"
                     /inference="COORDINATES: protein motif:HMM:NF045593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bilirubin utilization transcriptional regulator
                     BilQ"
                     /protein_id="HFG9718031.1"
                     /translation="DIVKHIIIIEGKFKMDFKNLQYESLSYIICNLQKNFKLYCEKCL
                     KPYKLTNGLYFYLIYINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPN
                     DSRAFQLRVTDKGEEVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVE
                     EE"
     gene            492..1586
                     /gene="bilR"
                     /locus_tag="ACGI57_002969"
     CDS             492..1586
                     /gene="bilR"
                     /locus_tag="ACGI57_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bilirubin reductase"
                     /protein_id="HFG9718032.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1674..2204
                     /gene="bilS"
                     /locus_tag="ACGI57_002970"
     CDS             1674..2204
                     /gene="bilS"
                     /locus_tag="ACGI57_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein BilS"
                     /protein_id="HFG9718033.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            2555..2890
                     /locus_tag="ACGI57_002971"
     CDS             2555..2890
                     /locus_tag="ACGI57_002971"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718034.1"
                     /translation="MPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWL
                     QIHAFYSDTIADYLTNFMCFVALSIEIFSIFFIYNIVRHVTIIIFIDILVFIALLIIG
                     VYKLFKKYN"
     gene            complement(3140..4516)
                     /locus_tag="ACGI57_002972"
     CDS             complement(3140..4516)
                     /locus_tag="ACGI57_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718035.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDEKDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDSIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            4784..5956
                     /locus_tag="ACGI57_002973"
     CDS             4784..5956
                     /locus_tag="ACGI57_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9718036.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYAIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            5970..6617
                     /locus_tag="ACGI57_002974"
     CDS             5970..6617
                     /locus_tag="ACGI57_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFG9718037.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            6619..7128
                     /locus_tag="ACGI57_002975"
     CDS             6619..7128
                     /locus_tag="ACGI57_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFG9718038.1"
                     /translation="MSQQNTGNVIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
BASE COUNT         2768 a          752 c         1256 g         2485 t
ORIGIN      
        1 tgatatagtc aaacatatta taattataga agggaaattt aagatggatt ttaaaaattt
       61 acagtatgaa agtttgtcat atattatttg taacttgcaa aaaaacttta aactttattg
      121 tgaaaaatgt ttaaaaccat ataaacttac gaatggtcta tatttctatt taatttatat
      181 taataaaaat aggaattgtt ctttgaatga tgtttctaca gaatttgaag ttgataaagc
      241 tcatacaaca agaactatat ctaggttaga acaagatgga tacatagaaa aaatccaaaa
      301 ccctaatgat agtagagctt ttcagttgag agtaacagat aagggtgagg aagtattagg
      361 ggatataaaa aacatatttt caaagtggga taatcacata aaaaaagagt tctcggatac
      421 agaatataaa gaactggtaa agaatttaca tgtggtaaag gatataaaaa cagctgtgga
      481 ggaagaatag gatgtattgt aatttattaa aaccaattaa aattggaaat ttggaattaa
      541 aaaatagagt atcttttgca cctacaagca tgggtcttaa attggaagaa aaaattaaaa
      601 agttttctga tattgctaag tcaggagtag ctctgattac tctaggagat gtatctatta
      661 gacctagttt tcataaggta gctataagcc tatcggatga agatggagtc atgaaataca
      721 agaaaattgt agatgaaata cacaattctg gtgcaaaggt atcagcacaa ctattttgtt
      781 cagattatga tgttaattta attaaagata ctatgaatat gggaattact tcacatgatg
      841 agataaaaaa aataatgaat gacggagtaa aagattacat aacaaatatg cccaaagaag
      901 aaattaaaaa tattataaat ttatttaaag taacagcatt gaatgcaaaa aaagcaggtt
      961 ttgacatgat tcaaattcat ggtgataggc ttgtaggaag tttttcatca agtatattta
     1021 ataatagaaa tgatgaatat ggtagaacgt gtgataatag aagtagattt gcaagcgaaa
     1081 taattagtag tatccgagat gaagttaagg acattccaat agactataaa tttgcaataa
     1141 gacaagaaaa tcctcattat ggaaatgcag gagttttact ttcggaagtt gagtattttg
     1201 ttaaaaaatt tgaatcccta ggtgtaaaca gttttcatgt aacacttgca aatcattcaa
     1261 aattagaaga tactatacca accaataatc atccttactt taaagaagaa ggctgttttt
     1321 tatatttagc tgatgaagta aagaaacaca caaatcttcc agtatgtggg gttggaaaat
     1381 tgtctagccc agattttata gaaagtatta tttcaaataa tagagtagat atggtttcta
     1441 tgtcaagaca attactagca gatagtaatt ggttacagaa agtaaaagat ggaagagtag
     1501 atgagattaa aaagtgctgt tattgtaata agaagtgtgc agatgcttta caaacacgct
     1561 ctcagtttgg atgtatacta gattagaata tccttataaa aataaataat ttgttaaggt
     1621 taatatattt ttggtgattt agataaatat ttagataaag aggaggagac atcatgaaaa
     1681 gtttagtttt atattcaagt ttaactggaa atactaaaaa gattgcttat actatatatg
     1741 atgaaattca agaagaaaaa gacattaaag atgtaaatga attagttgat tatggtattg
     1801 actatgaaaa ttatgatata gtattcttag gctattgggt ggacaaggga atttgtgata
     1861 aaaattcaaa acaagtgctt gaaaatattc acaataaaaa gatagcacta tttggaacaa
     1921 tgggagcaag taaaaaagga agttatggag ctagtataat agaaaagata gaatcaatta
     1981 tcccaaaaga taatgaaata ctaggttcat tcatatgtca aggtaagata gcagaaggat
     2041 tgaaagcaaa atataaagag atgttaaaac tttctcctga caacgaacat attcgccaac
     2101 aattaaataa ccatgaggaa tctcaaagtc atccagatga acaagaaata tatgaagcca
     2161 gtatgtttgc aaaaaatatg atgattaaag ctagtattgt gtagtagtta aacaataagt
     2221 tcatataaga tatagaataa ataatttagt aaaaaagtat agcaaataat ttgctatgct
     2281 ttttttatga aatcaaaaaa ataataaaaa tacttgactg taaaccttgt ttatacatta
     2341 ttttatagaa aataagatag ttttaaatat aaaaaaagat atattattga aaggagatat
     2401 tattgtatga aaacttataa aagataatta gtttgatttt aagatatact aaatatacaa
     2461 ttttatagag tgaaaatatt taagactata ttatagttat aaattaatag aatagagagg
     2521 tagtgtttaa attgcaataa cagtatttca aaagttgcca cctcaaattc ctatccaatg
     2581 gaataatggt gtagctaatt atgtagataa aaaatttatt tatgcctacc ctgtcatttg
     2641 tgttgtattc agaatacttt tacgtccatt tatatggaga tggttacaaa tacatgcttt
     2701 ttatagtgat actattgcag attatttaac gaattttatg tgttttgtag cgttgtctat
     2761 agaaatattc tctatatttt ttatttacaa tattgtcaga catgttacaa taattatttt
     2821 tatagatata cttgtattta ttgctttact gataataggt gtgtataaat tatttaaaaa
     2881 atacaattaa tttttaaaaa taagtaataa gtgatagtgt aaaaagtagc cactgcaaat
     2941 tatggattgt agtggctatt ttattatttg tatatgaaat tattttgttt ttctaataag
     3001 ttacgatata ttatggttta tttcactgat aaaaggttta ggtattttaa agtaaaaagt
     3061 tgtatattct aataaaattt aaacattttg attctttaaa attagagaaa atacattgct
     3121 tttattaaaa aattaaatat taagaattta atttctgcat cttatttaga aaagataaga
     3181 aatcaacttt atatattttt tttggccttc ctctttgatt atcgagtttt gtatttacaa
     3241 tagttgcaat tcctgcttta tataagatac tcaatattct attagcactt ctaagagtta
     3301 tgttcatata gtctgctaat atttctgctg acacattatt tgaatttcta tcattaatca
     3361 tacacatgat tttagataca tttgtattag atagaggtat aaaagaagca actttattgg
     3421 ctattgagct atcttcaata ttattagaat ttttatttga atataaaggg cctattgtat
     3481 cattagtatc atttactata aaagtacagt ttccattcaa agaagcagat ttaccattag
     3541 cttgacgagc attttgctct gcatgaatat ttgtatttcc aatgccccaa cctatattta
     3601 agctatattt aatttctttt aaatcttctg aaataaaaaa atctgtatag ttattggtca
     3661 tattgtttaa catttcttta gtagtgtgta tctctatttt catatcattt tttttgacct
     3721 gaactccata gaatccatgt cttgatagag aatttaatat agtgttatat atttgatttt
     3781 gtatctcatc atttttaaat atcaaatctt catttatgta ctctgaacta tttactgtaa
     3841 ctatacaagt tgcagattta tttttgtcta agttttgtaa tgttatttca ttcaccagag
     3901 atttaaatgt attcaggatt gattctgtag atggaaccat gtaaatagaa tcatatccaa
     3961 gtttattaag ttcacaacta atgatgtaat ttctagtaaa agaaaggtta atcttattat
     4021 ctcgcaataa gtctaaatgt atttccaata atttatcatt taatttttta aagtcatata
     4081 caggttgagg gatattaaaa tctttaattg tatatggaca tttattagag tctattaatg
     4141 aatatatatc ataataattg ttgtcctctc ttaaaaaatc cataaatgat tttgtaaagt
     4201 caatatcttt ttggctattt aataatttga ataagtattt ataaaaatct ttttcatcaa
     4261 gctttaaaaa atttaatgga acttttatgt cttttatatt tattataagt tcactatatg
     4321 gaactatacc actgcataca aatccatcaa aattatctac ttgctcatag tatatattag
     4381 gtaaattatt aaaaggattc tcccctttaa gctcaataaa tgtaatttca caagtatcat
     4441 ttaattcttt aagagcttgt ttaaacttat ctgacataaa ataagatgtt gtcaaaaaac
     4501 caattttata tttcataaaa cctcctttaa aatttcgaga gtattgtaac taatcaatca
     4561 atttataacc tcattttata atagtagata aaacgcgtca atttatttta caaaaggaaa
     4621 attgaaagaa aaatcagaaa atataaaaaa gactgaaaaa tatgttgact gtcaagaata
     4681 attgtgctat tttgtaatta aggactagtc ttttaagatt tcctaaaata aacttagatt
     4741 ttaggaaaga aatacatttt atttgaaaat aaaggagaag attatgaata catatttaaa
     4801 tgaaatttct aagtttgttg atgagaatag agaagaaata gtttcattat ggaaagagat
     4861 tgttaatatg gaaagctata cacattgtaa agaaagcgta aataaattgg ctaagagatt
     4921 aaagtttgag tttgaaaaag aaggtttaga ttgcgagtta gtagatgttg gagataatgg
     4981 tagtacattg ataggtacac ttggttccaa tatagataaa aagccaataa ttttttcagg
     5041 tcacatggat actgtctttg aaacaggtac atttggagag aatccattta aaataataga
     5101 aggaaaagca tatggcccag gtgttttaga tatgaagggt ggtataataa tttccttata
     5161 tgctattaaa gcattaaata aaataggata caaagaaaga ccaataaaaa tagtattttc
     5221 tggagatgaa gagataggac ataaggattc aacaggtgca gatgttatat tgagggaagc
     5281 aaaaggtggt ctttgtgctt ttaatatgga aactggatta gtagataata gtttgtgtgt
     5341 aggtagaaaa ggaagaatag gatgtaatat acatgtaaaa ggtgtagaaa cacacgcagg
     5401 taatgatttt gaaggtggaa gaaatgctat agaagaaatg gctaataaga tactacgcat
     5461 acaaaaattg actaatttag atgttggaac tacagtaagt gtaagtgtga ttaagggtgg
     5521 aagagtggaa aattctattc cagaagattg tagtataaaa gtagacctta gatttgaaaa
     5581 agtagaagaa atggagaatg ttaaagaaca agttagagag atatgtaaag aaacttacat
     5641 agaaggaact tctacagaag ttaattttat aagtgaaatg atgccatttg aaactacaga
     5701 agatgttatg agatttcata catttgtaaa tgaagtgtca aaagaaaatg gatttggaga
     5761 attaaatgct aagagacttg gaggtagttc agatgcaagt tatttaacta ttgcaaacgt
     5821 tcctacaatc tgttcatttg gtgtaagagg agaatggaac cacacatcaa gagaatatgc
     5881 agtagtagat tctatgtttg agagggtgaa attaatttct acagttattt taaacttaga
     5941 taaatttgag gtataaaggg ggattttata tggatgtaac agctggagat aataaggtaa
     6001 catggaaagg gtatttatct ttatttgtat tgatagtatt attttcagga gtgtttaaag
     6061 atagtcaagg cttccttaaa gcattagatt ttgctaatct gacaggttca tttggtacta
     6121 ttgcagatgg tgtagacttt atgggtaaag gaggaacggg agcaaaagat ggtttcttat
     6181 ttgctctgac tttgattcca actgtctgtt ttgctgtagg tcttatagat gtagttgaat
     6241 ctatgggagg aatgaaagcg gcatcaaaga tatttaatcc attgcttaga cctctgcttg
     6301 gtatacctgg aatagcagga atagcatttg tatcttcatt tactagttca gatgtagcct
     6361 caataatgac gaaagaatta tttgaaagtg gggaaataac agatgatgaa agaactatat
     6421 ttgtagcata tcaatatgct ggttcagctg ttatactgaa cacaataaat actcaagctc
     6481 cacttttacc aatagcatta ttggcattag gtccaataat acttatagag atattctgta
     6541 aaatacttgg tgctaatcta gttagattaa tcataggtgt aagaaataaa aagaataagg
     6601 ctaaggaggc tgtgtagtat gagtcaacaa aatactggaa atgtaattgt aaatagtaaa
     6661 cctaagaaaa ctgctgttga gatatttatg gggggagcaa aaaaaggttt ttacataggt
     6721 gttgaacaga tattaccagc catgataatg ggttatgtaa tagtgcagtt tttacaatta
     6781 actggtttag ttgatatttt aggaaatatt tttggaccag taatgggtat ttttggacta
     6841 cctggggaat ctgttgtagt tttgatagca gcattctttg ctaaggcagc aggagcagct
     6901 acagctgcaa acttatttgc acaaggtcta atcactgcac cacaagcaac catattaatt
     6961 atgccatgta tgttaatggg tactctagtt ggtcattatg ccagaatagt gcttgtagca
     7021 aatgccagta ctaagtatag aatgttactg ttggcagttc ctatatttga tgcaatagtg
     7081 gggatgttga tagtaagagt tttactcact gttatgggat taatgtaaat aacataaaat
     7141 tataacctaa ctataaatga tatataagaa ttaatatata caaggattaa tgagtagtaa
     7201 ttgatatata gtaataaact aagatagtaa ttaatgtgta tattaattaa aaaaagagag
     7261 t
//
LOCUS       DAYAOX010000187         7248 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.188, whole genome shotgun sequence.
ACCESSION   DAYAOX010000187 DAYAOX010000000
VERSION     DAYAOX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.188"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            129..626
                     /locus_tag="ACGI57_002976"
     CDS             129..626
                     /locus_tag="ACGI57_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9718039.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            676..1200
                     /locus_tag="ACGI57_002977"
     CDS             676..1200
                     /locus_tag="ACGI57_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFG9718040.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            1512..2876
                     /locus_tag="ACGI57_002978"
     CDS             1512..2876
                     /locus_tag="ACGI57_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFG9718041.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            3015..4181
                     /locus_tag="ACGI57_002979"
     CDS             3015..4181
                     /locus_tag="ACGI57_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFG9718042.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            4298..5080
                     /locus_tag="ACGI57_002980"
     CDS             4298..5080
                     /locus_tag="ACGI57_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9718043.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(5199..5852)
                     /locus_tag="ACGI57_002981"
     CDS             complement(5199..5852)
                     /locus_tag="ACGI57_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9718044.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(5876..>7248)
                     /locus_tag="ACGI57_002982"
     CDS             complement(5876..>7248)
                     /locus_tag="ACGI57_002982"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFG9718045.1"
                     /translation="KTMLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAA
                     TIAATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPA
                     DYIETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVI
                     IIYTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGN
                     KSISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIG
                     AIGGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRD
                     IYNKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVI
                     FAPFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVS
                     KFSKKD"
BASE COUNT         2765 a          865 c         1142 g         2476 t
ORIGIN      
        1 gttttgccgt ttataataaa aataaattta ttgacataat agtaactaat tactataata
       61 taaagtaatt agttactatt taaaattatc aaaaaattaa aatctaagat aaagagaagt
      121 gtataataat ggatgcagaa agtttaatga aaaaatatat ggtagatgat gatagtagaa
      181 aacaagctat atttgcaagt atatttgtaa tgcaaaataa acttcaaact acatgtgata
      241 aattagaccc agaattaaca atgaaacaat ggttactttt agctattgcg gactctgtag
      301 atgaaactct tacattaacc aaacttggag aattaatggg gtgctcaaga caaaatgtaa
      361 aaaaactagc tattgcttta gaaaagaaag gttttataca aattgagcag aatgaaaaag
      421 attcaagagc tatctgtctt gtgacaaaag atagagtgca agagtattct aaaaaagtaa
      481 gaggtatgca ggaaaaagta ttacagcttt tatttgaaga ttttacagag aaagaagtgg
      541 aacagttatt ttttggaatt atgaaattaa gtataggaat tgaaaatgtg aaaaattatg
      601 tagaaaataa tgacgttgga gaataagcaa gtatcaaatc aactttgtat gacatcagag
      661 agggggataa tttatatgaa aaatgtagta gtgatttata agagtaagta tggttcaaca
      721 aaaaaatatg ctaaatggct ttcagaatta ttgtcttgtg atatttttga aactaagaat
      781 attaatatag aaaaggttat gaagtatgat acgattattt taggaggagg tctttatgct
      841 tgtaatatag cgggaatttc tttcttaaaa aaaaattata aagaattgca gaaaaaaaat
      901 ttagtagtat ttggcgtagg agctgctcca tatgatgatg aagtaataaa gaaattaaga
      961 gaagtaaatt ttaaggatga attaaacaaa attccttgct tttattgtcg tggggcatgg
     1021 gatgagacaa aaatgtcttt tatagattat actctttgta gtttttttaa aaaatctata
     1081 gctaaaaaag aacgtcaagg tcttaaacca gcagaagcac ctttattgtc tggagaagcc
     1141 tttgattggt gtaataaaga aaatttaact ccaatacttg attatataag agaactttaa
     1201 gaggtatata tttgtaatta tttattatct aaagttagat ttcatagaaa cttactagac
     1261 attatttata aaataatatt attaaagatg gataatttaa ttgtttcaat aaggttataa
     1321 ttttaaaaaa tgtttatttt aaaataaact attttataag cttacttata cttaataagg
     1381 ggcttttttt attggaagat ttatgttata ttgagatgaa tttattatga tataaaaaat
     1441 aatgttagga taaatttaaa tctttttcta aaaatgtatt agtataattc ttaaagaaag
     1501 gatttttaac aatggatata aaagagttag ttaaaatgca aagaaaatat tataatacag
     1561 gtaaaacaag agatatatcg tttcgtatag aacagttgaa gaaacttaag ttagttgtat
     1621 cacaaaatga agaaaaaata cttttagcac taaagaaaga tttaaataaa tcggattttg
     1681 aaggttttat gacagaggta ggcatgtttt atagtgaatt aaactttgct ataaagaata
     1741 taagaaaatg gagcaaaata aaaagagtta aatcaagtat ggttaatttt ccatctatat
     1801 ccaaagttgt tccacaacct tatggtgtta ctctcataat gtcaccttgg aattatccat
     1861 ttcaattagc acttatacca ttagtttggt cattagcagc agggaattgt gttatattaa
     1921 aaccttctga atattctgtg tcaacatcta gtgtagttaa agacatagtt gaagatactt
     1981 tttcaaaaga atatgttgca gttgttcaag gaagtcaaga agaaagtgaa aagttactat
     2041 tggaaagatt tgattatata ttttttacag gaagtactaa tgttggtaaa ataatcatga
     2101 aatcagcatc agagcattta actcctatta ctttagaact tggaggaaaa agtccttgta
     2161 tcattttaaa agatgctaat atagacctta ctgccaaaag acttacttgg ggaaaactaa
     2221 taaatgcagg acaaacctgc gtggctccag attatgtttt agtgcatgaa gatagaaaaa
     2281 atgagttaat agagaaaata aaatattata ctaataagta ttttggggat aatgcatgta
     2341 ataatgagca atttcctaaa atcataaatc agagacattt taatcggata ttatctctta
     2401 ttgataaaga taagatagtc tatggtggaa attataataa ggagacatta aagatagagc
     2461 caactatagt agacaatgtt aattgggatg ataatataat gaaggaagaa atatttgggc
     2521 ctatatttcc cattttaaca tataaagatt tagatgaagt aatacaaaaa attatacaaa
     2581 tgccaaatcc attggcactt tatattttta caaaaaataa gtacctagaa aataaacttt
     2641 tagaaatgat tccagcaggc ggatgttgta tcaatgatac agttactcat atagctacga
     2701 attacttacc ttttggtggt attggtgaaa gtggtatggg ctcatatcat ggaaaagctg
     2761 gattcgatac atttactcac tataagagtg tgttaaaaaa attaaatttg gatgtcccta
     2821 ttagatatgc tccatacgat aataaattaa ttaaggttct taaaaagata atgtaggtta
     2881 tattgtagaa taataaatat aaataaaatt gagtatttgt attctgaata tattttattt
     2941 ttaatttggt atatttgtta taaaagtggt gattttgtga taaaatttaa ttatatagaa
     3001 ataaggagga aaaaatgaaa attaattgga atacaaaata cacaacaata gcaatttata
     3061 catttataat agctgctagt agtattatat tttatctagt atcatcacaa attgatgtat
     3121 tttctaataa tttagatgct atatttacaa cattacaacc ttttataata ggatttgcta
     3181 ttgcatatct tcttaatttt attttaaagt tttatgaaga cagaattttt ataaaaagtg
     3241 agaaattaaa aaaactaaaa caaagttcaa aaagaggctt gggtctttta ttaacatatg
     3301 caacagctac attgatactt tacttattta tgcatttcgt attgccacaa gtaattgaga
     3361 gtattgttgg actagcaaat gatataccta tgtatgtaaa caatgcaact aagcttattg
     3421 ataaattaat gactgattta aatcttgatg agcaatactt taatttggca gttgataagt
     3481 ggaatgaatt tgttacatat attataaagt ttgtaacaga tttaatccca atattaggaa
     3541 gtatgttaaa gaatgtagcc tctagtatat ggaatattgt actaggatta attgtttcag
     3601 tgtacttatt aattgataaa gaaaaattct atggattaag caaaaaaata acttatgcag
     3661 tatttacaga aaaacaagct gccagaattt tagaattaac tcatagaagt aactatacat
     3721 ttggaagatt tttaggtggc aaaatattag attcattcat tataggtatt ctaacatttg
     3781 taatacttac gttagttaaa atgccatata cattattaat atcagttata attggtatta
     3841 caaatattat ccctttcttt ggacctttat ttggagctat accatctacg ctaatagtga
     3901 tgtttgtatc tcctataaaa gctttctggt tattgttgat tatattaata atacaacaga
     3961 tagatggaaa tattatagga cctaaaatac ttggagattc aattggtata tctgcatttt
     4021 ggattttgtt ctccttattg gttgcaggaa aattattagg ttttattgga atggttatag
     4081 gagttccaat gtttgcagta atttattcta taattaagga tattgttgaa agtaaattgg
     4141 acaaaaaggg attaccgaca gatacttccg attatatgta gctaaaaaat attatgtata
     4201 attaaaaata tataaattaa gcaatattta aagcactgag aaagttaaac tcttggtgct
     4261 ttttagatat aaaattatta tagaagaggt gttttttgtg gcatttatag aatgtaaatt
     4321 taaatctaaa tatactggag gagaaactga tataattgta attttgcctg tagcaaatgg
     4381 aaaagaactt tttagtggta aagagatata tgattttgaa ggtaaattta aaacattata
     4441 cttatttcat ggtttagaag atgaccaaag ctcatggatt agatatacta atgtagagca
     4501 ttatgctaaa aataaaaata tagccctagt aataccaaga gtagaaacta gtttttatac
     4561 tgatatggcg tatggacacc aatattttac ttttgtaagc gaggaattac ctaaatttgt
     4621 tagagctata tttcctttat cagataaaag ggaagataac tttgtagctg gtatgtctat
     4681 ggggggatat ggagctttaa aattagcgct ttcaaaacca aaagagtttt cggcagttgc
     4741 aagtttttct ggttcgcctg atatgattca tgagttagaa aatcaacaaa tagaaaccaa
     4801 tgcagacatt ctatttcacg attttggtag accagaagac gctagaaata gtgagaatga
     4861 tttactttac atacttaaga accttaaaga aagaaatgaa gatattccta aaatttatca
     4921 attttgtggt gatagagatt ttttatataa aaataatcag atgtttaaaa aatatgcaga
     4981 ttctctgggt gtagatgttg aatatgaaga gtgtgtaggg gggcatgaat ggcgtctttg
     5041 gaatttatgg ataaagaaat ttatagatat aattggatag tacacttatg gtattaacag
     5101 gtatactata aaatatcttt aaatatgata aagactgaaa tcctaaaatc aaattatgat
     5161 tttaaaattt cagtctctaa gaatttaata aaattttatt aagttcttac acgagttatt
     5221 attggagtaa aattgtcaac tatatctata tagtatttac tcaattcctc taaaatctct
     5281 tcgcataaat ccgtactatg accaagatta ataagaaact gttttgtaaa atgctttatt
     5341 acagcttgag aagatatatc gtacttgtgt gaagtataaa ctctaattat ttcaggtttc
     5401 atagcaagta taccaataca agaaagtatt aattcatcat gttctataga ttctttatca
     5461 ttaacatatc ttttcatgac catttcggtt acacttcttt gatagcttaa aatagcttct
     5521 tcataagcta tatccaaata aaaatttcta tatgcttcgt tttttagaag gaaacaaagg
     5581 tcagcattca tagcaagaaa gaacatcatc atagtacctt cttcatggta gttatagatt
     5641 aaagactgca tttcatttct tatatcttca tatattatag ctcctatttc gcttttactt
     5701 gagaagtaat agcttattag acctaaattt atattagctc tttctgcaat ttgcctagtt
     5761 gttgtcttat gataaccttg ctcaaaaaat aattctctag cagcacttat aatactagct
     5821 tttgtttctt tgcccttttc tgattttgac atatttactg tgtatacctc tcttttcaat
     5881 ctttttttga aaatttgcta actataaata ttgtaataga accacctatt ataggaacca
     5941 aaattctgtc aaatacagta agaataccaa tcatatttaa taatgccaat ataacactta
     6001 ctataacagc atagatagct cctttttttg tagcaccttt ccagcaaaga ccaattacaa
     6061 atggtgcaaa tattacacct gtacataatg gagctggcca aaacaataat tctaataaac
     6121 tatcaaattt aagtgctatg attgcaccta ttatagctgt caaagctgat gcaatttgag
     6181 aatactttag agtatttttt aaatcattaa attctatatt aggatgtaat aatttgttat
     6241 aaatatctct tgatataata gcactcattg aggtcattaa tgaatcggca gtagacatta
     6301 tggcagccaa tatagcagct aagaataata cagatattaa aggtggcaaa gtagtcgata
     6361 tcatttgagg taaaacctga acagatgata tatttggaaa taaactacca ccaatagctc
     6421 caattattcc tatagggaaa gcaagaaata atagtataaa tcctgcaatt acactacttt
     6481 taactgcatt tttagcatta tcagcacatt gatatcttaa aaaactttca taagaaaata
     6541 ttgagcctaa aactaatggt aaaactgtat acataaaatc actaatcgat ttatttcctg
     6601 gaaagaatgg ttcttttacc attgattgta acataagtaa agggttgtcg ccaactaatt
     6661 ttaagcttat ataagctact attggcaaaa acactataca aatagcaagt tgaataaatg
     6721 ctgatactgc aactccccac attcctgatg tataagtata tataatgata actataactc
     6781 ctataattac agctatatca taaggaatac caaatgcagt agatacagaa ccaaaagaaa
     6841 tcaattgact acccataata gctagtatag atattggtgt tacaagactt tgataagctc
     6901 ttaaaaatac actatgacca tatctagttt caatataatc agcaggtgtt atatcacaag
     6961 ataagcttct taattttcct gcgaaaaatg catttataaa gcaagctaaa gctgcaccta
     7021 tagtagaata cattccaacc caaagtcctc tttcagctcc ccattgaact ccaccaacta
     7081 agcaccctcc tccaaaagat gtagcagcaa tggttgcagc tagtataggc aaaggcagat
     7141 ttctacctgc gattaggaaa tcggatgctg ttttaacttt ttttgaaaaa taaaaaccaa
     7201 tccatatgta tattgcacac gtaacaatta aaagtaacat agtctttt
//
LOCUS       DAYAOX010000188         7221 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.189, whole genome shotgun sequence.
ACCESSION   DAYAOX010000188 DAYAOX010000000
VERSION     DAYAOX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.189"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            100..1371
                     /gene="sleC"
                     /locus_tag="ACGI57_002983"
     CDS             100..1371
                     /gene="sleC"
                     /locus_tag="ACGI57_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic germination protein SleC"
                     /protein_id="HFG9718046.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(1797..2453)
                     /locus_tag="ACGI57_002984"
     CDS             complement(1797..2453)
                     /locus_tag="ACGI57_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718047.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGVRILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(3565..3975)
                     /locus_tag="ACGI57_002985"
     CDS             complement(3565..3975)
                     /locus_tag="ACGI57_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718048.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(4191..5573)
                     /locus_tag="ACGI57_002986"
     CDS             complement(4191..5573)
                     /locus_tag="ACGI57_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9718049.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVILRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(5584..5943)
                     /locus_tag="ACGI57_002987"
     CDS             complement(5584..5943)
                     /locus_tag="ACGI57_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9718050.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(6148..6927)
                     /locus_tag="ACGI57_002988"
     CDS             complement(6148..6927)
                     /locus_tag="ACGI57_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFG9718051.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         2563 a         1212 c          750 g         2696 t
ORIGIN      
        1 aaaatttaaa tttatatcta tattatttaa caaatttcaa gctatattca tatcttgaaa
       61 tataagggaa ctaaattcat ttaaagaaag ggtgatttga tgcaagatgg tttcttaaca
      121 gtaagcataa ttgatgctac taacaataga cctattcaaa atgcagtagt aaacatatat
      181 tcaatgtcta atggtagcca aagctcatca actttatacc aaaacctaag aagtaatgaa
      241 tctggtcaag tgactggttt agtattacca gcaccagatg tagattactc tctacaacca
      301 tctgatgtta gaccttatag ccagtatata gtggaagcca tagcagatgg atatgagaca
      361 gttgtcatag aaggaacaca gttgttagct acaattgaag caagacaagg agttcccatg
      421 tctcctagaa caagaagtaa gcgttctttc tcaagacaaa gtgagttgat ttttgatata
      481 ggagaacata ctctatatgg tacttatcct cctaagattc cagaaagtaa cttaaaacca
      541 ttaccacctc ctactggttt cgttgtatta gataaccccg tagtgcctga atttatagta
      601 gttcatgatg gtttgccaga agattcatca gctccaaact attggattcc atttaaagag
      661 tacattaaaa acatagcgtc atctgaaata tattcaactt ggcctgaaca gacaatctat
      721 gcaaatgtaa tagctataat atcatttact ttaaatagag tctttactga atggtataga
      781 aataaaggat ataactttac aataacttca acaacagcat acgaccataa gtttataaat
      841 aatagaaact tatttgaacc aattaacgta gttgtagatg ctatttttaa tacttttata
      901 aaaagacctc ctacatcaag acagccacta ttagcacaat attgtgatgg acaaaaatcc
      961 caatgtcctg accaaatgac tcaatggggc agtaaagact taggtgacca aggatatgac
     1021 tatgagagta tactaagata tttctatgga gatgagatag tatttgaaag agcacctata
     1081 gtaagtggtg taccagtgtc attccctggt actacactac aagttggttc ttctgggcag
     1141 tatgttagaa ccatacaaaa tcagttaaat gctatatcta attcttatcc agctgtacca
     1201 aaagttatag aagatggtat ctatggaaca gatacagaaa acgctgttaa gatattccaa
     1261 ggaatctttg gtttacctca aagcggagta gtagatttta aaacatggta tgaaatatcc
     1321 agagtttatg tagctacaac tagaatagct tctttaaatc ctttaattta aagcttgatt
     1381 tagaacataa actcaaatta ttgaataaaa ttattaattt aaaataaaag tggctgttat
     1441 gaactaatat ataccataag tattacttat aagtattgta gtcttaacag ccatttttta
     1501 gttataactt gtttttttag aatcttaaaa ctttaaatgg gggattttaa gcttcttaaa
     1561 aacagtgctt agcaacttag atttctttaa aatattatat ttatttttaa gtttaataaa
     1621 atgctattct aacgaaaaac ctacttttta tttaaaatct cacttattat ttaaaacact
     1681 atccatcact tagaatatta cttcttatat aaattattat ttattataaa tacaaataat
     1741 attaatttaa aaagttattc ctaatttaaa taatactact atcttcatca gtgaacctaa
     1801 actgcaactt tagacttatt agctaaagcc aatattagac ctaaagctat agatgcaaat
     1861 gcagaagcaa ttactctata ctcagcaggg aataaaaata caattgtatg acatggtatc
     1921 cagaaaaatg gtatagtttt taaaacacta aaaccaaggt aagcattcca gtccacaata
     1981 tttaccacat ctttaagttt taccttaatc ccctttccaa cctcatattt tttatctata
     2041 aaacagtcca taaccttatg gaataaaaat acagttggtg acattagtat attcatagtt
     2101 gcactcttaa atagagcatt tgcaaaagct attccctcaa atggaagcat accatccact
     2161 tgtgcttgac ctacacctga tgagtagatt gacattccat atgtggtaac tacacctatt
     2221 attccccaaa ctattgctct ataaaatact cctactggta ttttccaatc cttatacaaa
     2281 attctaacac caagtaaatc tcccattgta gctaatacag caaactttaa aaaacctcca
     2341 atatatggat gtgcacctgt tgcttccata aatacttctc tactacttgg tattgctaag
     2401 acagcaataa tacaaataag aactccactc catataaaat cgcctttttt catatttata
     2461 aatactattt aatatataaa tagttctcct ttctattaaa gatttattta atttacaatc
     2521 tatctacaat tatttattaa tcacttatta ttagatattc attttccata tttaaattta
     2581 ttctatccct ttatctattt attttcttac tactctatcc accattatct atatatttat
     2641 tttacgatta ctctttcacc ctaacccttt aactatctat tttttcttac tattcttatc
     2701 cacatcatcc atatatttat tttacgatta ctctttcact ctaacccttt aactatctat
     2761 tttttctgat attcttatcc accatcatcc atatatttat tttacgatta ctctttcacc
     2821 ctaacccttt aactatctat ttttttctgg tattcttatc tactattatc tgtatattta
     2881 ttttatgatt actcttttaa cctaccccct atactttata gtaatatgtt tttaaccttt
     2941 ttcattattc ccaatatgct ttataactac aaatactctc cccccttaaa aataataata
     3001 aaattaatca ataaatttat aatcatatta ttactctatg tattaagcaa ttactatgcc
     3061 aataaacttt gattttttta aacaaatctt aaaaatccta aaatgctaat tttctttaac
     3121 ttttaatgaa tctaatagat aacatttaat tttcgttata tttttcagaa tatttctatt
     3181 attattttta agaaaaaaaa ataatgttaa catttcttaa catacattaa aaaatgttaa
     3241 cattataaat tgggtttaat acttttactt tgactcgatt tacttttatt ctacttctta
     3301 ctttattttt aacttagact ttattttttt attacttatt tgagtcttat ttattttatt
     3361 ctcactccat atgttcaatt acatttgtct aaattctttg atacttttat tacataattt
     3421 taacaagctt aagtgttatt tgggtagtgc agttgataat tatttaaata atcatcaact
     3481 gcatgtttat atcattacag tagcttaatg tatttaatca tacttactta ggattttgaa
     3541 atatcttaga gattatattt aacattagtc aattgctgca taattgtaag caattgaaat
     3601 ttgagaccca tcttcatttt ctatagtgat aggcttcata atttttaatc cttcagtttt
     3661 tatcttttct aattcttgtt ccattttctt aattgtttcc tcaaggtctt tttgacttac
     3721 atctttgact gcattagggt ctttagcata gtcttttaat tcttgaatat gtttagcata
     3781 ctcttcataa gtataatgct caacaccata cttttccaat tcagtagggt cagttattcc
     3841 tgattttatt agagattcaa atttaggtaa agcaatatta gatgaatcat ttccatttgc
     3901 aaatactgct ccagttcctc ctagtaccat tgcagatacc atcattgcac ttataaggga
     3961 tttttttatt ttcataacca atcttccttt cttagagatt tatttgggta gtgtagttta
     4021 aaatgactat tgcaatagtt atcaactaca ctcttagact atcacagtag tcatgtttat
     4081 gtcaagcctt tttaaaaaac ttttcaataa aaaatactat ctcaaagcaa aattaattat
     4141 tttgagatag tattttaatt ctaaatcttt tatattacta agatttatgg ttattctatc
     4201 catgttctat attgttcttt attaactgct aacaaattcc cttttcttaa tatgacaaag
     4261 cgattatcat tatagccact tcgctcaatt gaaacttctc ctgaatcagt ataaagtaca
     4321 tccttactat tactagattt atttacatac gtttcttcac tattctcaga attagttact
     4381 ttgctcatag atgattcttc atgctctcta aattcaatta cagatacttt tgccgtacta
     4441 aaatgagtct ctccatcact tttagcttta ctaacaagaa tatcagattc tactttggga
     4501 gaatataaat cagttgattt actacttatt gaaaatccaa ttttatcact ttgttcattt
     4561 tttgttaaac caaaagattc aacctcatta atctctttat tttctgttgc aaaaacaaga
     4621 cctgtcccac aaactaatac agttgaaact aaaaaagtaa cataagttga ttttttaaat
     4681 ttcataatat taactattct cttttctatt tttgtctttc caaatccact acatattgtc
     4741 attgaaaatg gattattctc ctctgctaga cttattagtg tggttgcata atcagcccta
     4801 tattcttcac caagtatact cataaccttt tcatcacaac taaattctat gtctttattt
     4861 agtaaaaaaa acatcaccca aactaaggga ttaaaccaat ggacaacaag agctacaagc
     4921 ataaataact ttaacagatt atcaaagcta ttaatatgaa ccatttcatg agttaaaaca
     4981 aagtctaaag tttgattatt agcataattc atttgctttg gcaaaataat tcgtggtttt
     5041 aaaataccag atgtaagtgg tgtcctagta aattcagata tgcaaacaat aaatcttcta
     5101 aaacggctat tcttacacat ccatttttta atgaactcat gattttttat atgaattgaa
     5161 gttttaaaaa gattaattat tttaaaatat ctaaagcata aaagtatgct aaacataata
     5221 gtaactataa aagacaaaat gcccaacatt tccaacatca taatattttc aatattagac
     5281 attatttcaa attcgtcaga attgttttta cctgtagagt cactagcttt cttaagagta
     5341 taattttctg ttgatgtatt acccaattcc acatcaaaag tcgattcaat tatactataa
     5401 atactaaaac cagaatttaa acttactggt attagaagtt taaataaaac aatcaaccac
     5461 aataatacaa ataccttttt aggtaatttt ttaaataaac atgctcttat aaaaataact
     5521 attaaaagta atactccaga agtaaaactc atttctaaca atttaatgaa cataaatctt
     5581 acctcacttt aatttttcaa caatttgttt aagttttgta atctcatcac ttgaaagatt
     5641 ttctttatta ataaaagctg aaagaaattt ttctgaagac cctccaaaca ttttattaat
     5701 taaagtttct gtttcctgtt tttgtacttc ctctttgctt attaaagctt tacaaacaaa
     5761 attcggctca attctctcta ttgcaccttt tgacactaat ttttttataa cggtatacgt
     5821 agtgtttcta ttccatcctg tttcttcttt taaaattttt actaactctc cagccttgct
     5881 ttctcctctc tcccatagaa cttccattac tttaagttct gaatcaaata attttatctc
     5941 catataaaat cgctcctcaa atatagttat cacaatagtc attactataa taaaaaacta
     6001 tcatagtagt ctatactatg tcaatcattt attttttaat aataaaaact gtctttttaa
     6061 aaaattctta taaaagacag ttttatcaat gttatacttt aatgtttaat taaagccata
     6121 ttttatttaa atatataact atctatctta gtacatagta taagattttt ctttatctaa
     6181 tagtatatcc tctacatctg ggttttcttt tactaattgt ctataaaaat tacaattagt
     6241 aacacccaca tatggaatta gccatagtag acctattcca agagtagcta ttgaaagtat
     6301 ataccatcct ataaagctaa agtttagtac aaataattta agtacatttc ctttcatcat
     6361 atctaaacta gcacccattg atttaaatac acccatatta tccttgtctt cacaaattaa
     6421 gaaagtagct tgagaaacat ataatagcaa tatataaact ggtaacataa gaacaagtat
     6481 tataagaact agagttattc cagtagaaat aatagtccct attccaacat tatcacttga
     6541 taaatattca attgctccac tagtaagtaa aactccaaat gataaaaacg ctcctataat
     6601 tgtgattata aaagaataaa tatacacaat aattgattca aataaataaa aaccaaaact
     6661 tcttaaataa attctcccac ttacaaagat atctcctatc ttgacttttt catctttagt
     6721 aattttcaag aacatacatg taaatcctgc tgaaacgcaa aaagctatta cagccactac
     6781 aatagtagtc aatggttcaa aaggtaatcc tacaattgat attaatacta tcaatgcaat
     6841 atatactgcc atagaaattg cacatatccc ccagtttcca gataactgag ctttagatac
     6901 tcgttttaat tcttgccttg ataccataaa aaacacccct ttcattttat aaaaaaactt
     6961 ttaaaaatat aaataatctt ttaacgtata atattaatat atcatatttt cgactatata
     7021 agatacaatt attcaccatg ttttatcaag atatgccata attgtattac tagttttata
     7081 tagtcaacat taccaaatac atcaaataaa tttgattaat tattcaacaa gtaataaaat
     7141 aatttctatt aattatactt tatttagtaa acaaattatt taataaaaaa attacctaat
     7201 acagtaaatt aattggtaag a
//
LOCUS       DAYAOX010000189         7120 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.190, whole genome shotgun sequence.
ACCESSION   DAYAOX010000189 DAYAOX010000000
VERSION     DAYAOX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.190"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            454..945
                     /gene="infC"
                     /locus_tag="ACGI57_002989"
     CDS             454..945
                     /gene="infC"
                     /locus_tag="ACGI57_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFG9718052.1"
                     /translation="MNEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLDLVQISP
                     NANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTKANNAIK
                     FLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNNMVVIID
                     PKK"
     gene            973..1167
                     /gene="rpmI"
                     /locus_tag="ACGI57_002990"
     CDS             973..1167
                     /gene="rpmI"
                     /locus_tag="ACGI57_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFG9718053.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1223..1579
                     /gene="rplT"
                     /locus_tag="ACGI57_002991"
     CDS             1223..1579
                     /gene="rplT"
                     /locus_tag="ACGI57_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFG9718054.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1889..2434
                     /locus_tag="ACGI57_002992"
     CDS             1889..2434
                     /locus_tag="ACGI57_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718055.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            2421..2594
                     /locus_tag="ACGI57_002993"
     CDS             2421..2594
                     /locus_tag="ACGI57_002993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718056.1"
                     /translation="MKGNKSDLHGVEPIYTGNRFMIYICIQSKVSLLGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            2869..4794
                     /locus_tag="ACGI57_002994"
     CDS             2869..4794
                     /locus_tag="ACGI57_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9718057.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            5195..7099
                     /locus_tag="ACGI57_002995"
     CDS             5195..7099
                     /locus_tag="ACGI57_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9718058.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         2999 a          713 c         1349 g         2059 t
ORIGIN      
        1 atgtagtata gtagtatatt agaatacaat atagaatttg ttgggtagta tagtgttata
       61 ttagaataca atataggatt tgttatgtag aatatataga gtcatgtaga atatacatat
      121 ataaaaggat tttgggtata aatttaaaaa ttaatagcaa aatgagtagt atagacatat
      181 aattagtagt aggaagtaat taaatttttc ctaaacaaaa aaaatagttg caaataattt
      241 gattaagtga tatagtaaat aaggtaagaa taaaacaaag aagaagtccg cttctcacct
      301 tacgccggag gttagtaagg taaatataag ttaaagctta attttaattt aagtttgttt
      361 aaatttggta acttatattg cggatgatta cgtcatccgc tttttatttt ataaaaattt
      421 tggaggtgtc ctacaattag caaagaatta gcaatcaatg aacaaataaa agataaggaa
      481 ataagagtcc tttcaccaac aggtgaacaa cttggagtaa tgcctactaa agaagctcaa
      541 gcaatggcta atagcaaaaa cttagatttg gttcaaatct cgccgaatgc aaatccacca
      601 gtatgtaaga taatggatta tggaaagttt agatatgagc aagcgagaaa agagaaagaa
      661 gctaagaaaa aacaaaaaac tatagttgta aaagaagtaa gacttagacc aggtattgaa
      721 caaaatgatt taaatacgaa agctaataat gctataaagt tccttaaaaa aggggataaa
      781 gtcaaagttg aacttagatt tagaggaaga gaattaggac ataaagacat aggtaaagaa
      841 gttatgctta agtttttaga tataataaaa gagtttggag aaccaacaaa agcccctgca
      901 tttgagggta ataatatggt tgtaattata gatccaaaaa aatagatgat taactgagag
      961 gaggaatttg tcatgcctaa aatgaaaact catagaggtg cagcaaagag attaaagaaa
     1021 acaggaactg gaaagttaaa gagagctaaa gcttttaaaa aacatatatt aactaaaaaa
     1081 tctgcaaaaa ctaaaatgaa cttaagaaaa tcaactttag taagtgatgg agatgctaag
     1141 agaatagcac aattattacc atactaagat acagattgta aataataact atagaagagg
     1201 gatataaaag gaggtcataa cgatggcaag agttaaaaaa gctatgaacg ctcgtaaaaa
     1261 gcataagaaa atattaaaac ttgcaaaagg attcagagga tctagaagca aattatatag
     1321 accagcaaat acattcgtaa tgaaagcatt aaaaaatgct tatataggaa gaaagttaaa
     1381 gaagagagac ttcagaaaac tttggataca aagaataaat gcagctgcta gaatgaatgg
     1441 aatatcttat tcaagattaa tgaatggatt aaaattatct ggtgttgaag ttaacagaaa
     1501 aatgttatct gaaatggcta tacaagaccc agaaggattt gcaaaattag cagaagttgc
     1561 taaagcaaaa ttagcttagt attgaatcat gagatgtaaa gtctttgagt tatataaata
     1621 attcaaggac ttttttattt tttgaaaaat acttattagt gtcatgtaca accaatttta
     1681 taatgataaa taaaataagc aatacaatag aattttatga gatttattat aagaattgtt
     1741 acaattattt atgatagcta taaaaagtca tagtaaaaat gatattgatg tatgttgata
     1801 tatgtataaa gtttccaatc aatcttgaat taatattatc aacatgataa tattatctta
     1861 ttaataatat aaataagggg gacataatat gaatgaaaaa ctaatggatt gttttgtaaa
     1921 cccaattaaa tgcaagttat tgattgaaat tttttcaaat aatcgaacta cagctaaaca
     1981 attaggagaa aaatttcaag atatatcaaa ggcaacactt tatcgttacc ttaataaaat
     2041 gttgaaagat gatattataa aagtggttga agaaaataaa ataaggggaa ctgttgaaaa
     2101 agtatatgct atgaagttag atatagctga tgatgtgaac aaaattatag atgagaactc
     2161 tggagaagaa tatgctcaac tgtttactca atatatgatg ggatttttaa gagaatttta
     2221 tgaatacgct gaaagaaagg aaaaggatat cctaaaagat ggttcaggat tttctgtctg
     2281 tccagtatat gctagtattg atgagatagt tgaagtgagt aaaaaaattg ctagtatact
     2341 agaacctcta agaaataact cacatgaaga aggtagaaga atgcattcta taggagtgat
     2401 aattactcca cctaaaaagg atgaagggaa ataaatctga tttacatggg gtagagccaa
     2461 tttatacagg gaatcgtttt atgatttaca tatgtatcca gagcaaagta tctctgcttg
     2521 gattgtactt cattgtacca tttcatccaa tgacttggag tggtacaatg aagtttttta
     2581 atttttcata ttaatgctaa agaaatgaac tttagaccac atatgcaatt aggttgcatt
     2641 atacaattag accacatata caaaataaaa atcagtattt ttatagcaga taaattgact
     2701 gtcattcttc ttaaaaatca tctattagtt attgttagca tattgaaaga aattatagta
     2761 atctattgaa taaatgaata ttgcctataa tcatataaac tgctatagtt taaaataaag
     2821 caggacgtta aattttatag taatctaaaa atttaagagg gggattttat gaaaataaaa
     2881 tttgttatta tgccattggt gatattttta atggtattat ttgtggggtg tcaaagtaca
     2941 aaagatacag aagttaaagc aaaagataca gaaattaaaa tattagggac aggtgattta
     3001 cattcaattt tgacagactc tatggtttcg tatgtaaatg aggaaaggga aaagaataaa
     3061 aatctgctta tggtagatgc gggagatttc tatggtacac aaagtagaga aatgtgggaa
     3121 tggtcttctg gaaaaaagtt aattaatatt aaaagaaatg gtagagcaga gtatgatgac
     3181 ataattaaga gtagtaaaga tgaagtacct atagttaaag atatggcaaa gttgaaatat
     3241 gatgctgtag ttttgggaga taatgaattt gtttcaaatg ataagcaaag tcttgataaa
     3301 ctggtttctg attttaaaaa taataatatg ccattggtat cagcaaatat atatgaacaa
     3361 tctggagaaa attatgtaca accatatgta atgaaaaata ttaagacaga tgagggcaat
     3421 gtaaaagttg gaatactagg tcttactata aaagaagtag gggaaaccct tggtcttgag
     3481 gattttgaaa aggataaaaa agctagagag cttggagaac aagctgatta caaaggaaaa
     3541 ttatatgcaa atgatttagt agaagatgca aaaaaatgga taaaagttat ggaaaaagaa
     3601 agtccagaca taatagttgc agttgtacac tcaggggaag aacctaaaac agatagaaat
     3661 ccaggaaata gaataaaaga gttagcaact acagtggatg gaatagatgc aattgtagct
     3721 ggtcatactc atgaaaaaat agatgaacac aaatataaaa ataaatctgg agatgaagta
     3781 attgttacac aaccaggttg gcatggagat agagtatcag agattaattt caaactaaat
     3841 aaagagaatg gaaagtggga cataaaggaa aagtctagta aattaaaaat aattgataaa
     3901 gaagtaagta tagttgctac tgcagattta cattcacatt tctctgataa attaacagtt
     3961 gaccttgtta atgaacgaag taacccaatt gaacctgttg tagtagatgc aggcgacttc
     4021 ttagaccctc aaacagatga aatgactcaa tggtataaag agtggaaaag tataagagat
     4081 aataatagtg ataaaacgat tagtagatgc ccaatggtca tggatatgaa tcagggaatg
     4141 tatgatgcag tagtacttgg aaatcatgaa tttgtatctg aaaaagatga attttggtca
     4201 gatgaacgcc ttttagatgc tgttatagag gattttgaac aggtggcaat ttctgtgtta
     4261 tcggcaaata tatataggca atctggtaaa aattatgtta aaccatatat tataaaagat
     4321 gtggagacga gtgaaggtaa tgtaaaagta ggaatattgg gacttactat aaaagaagta
     4381 ggtaaaggtc ttaaaaatgt aaacttacaa gagcaatttc aatataagga taaattgtat
     4441 gcaaatgatt tagtggaaga tgcaaagaaa tgggtaaaag ttatgaaaaa gaaaaaacca
     4501 gatataatag ttgcagttgt acattcagga gaagaaccaa aaaatccaaa acatccagga
     4561 aatagaataa aagaactagc aactacagta gatggtattg atgcaattgt tgctagtcat
     4621 actcatgaaa aaatagatga gcatgaatat aaaaataagt ctggagaaaa agttatagtg
     4681 acacaaccag gtgagcatgg taaatattat tctaaaatta cctttaaagt aaataaagaa
     4741 aagggttcgt ggactattaa tgataaattc agtaaaacaa ttgaggttga ataactttat
     4801 aaaatttata gataaagtaa attatctgat attggcatga tatataagat aaagtttcta
     4861 atattagtat aatgcataat gtgaaatatt ttaataataa gagaataaac aagacaaata
     4921 aaaatatttt aacatgtaag atatagtatg aactattata ctaatgctta ctttataatt
     4981 tgaacatgtt agaataagtc ttatttgaaa attttagtgt actagagtaa gtattatttt
     5041 actagtaaaa aatattgaga actatatgta gatttgctta aaaaggaatt tacatatagt
     5101 tcttttcgta taataaatag tacaatctta tatggtaaag ataaagtgtg atattaaatt
     5161 ttattatcat ccaaaatcta aggaggagtc tcttatgaaa ataaaattta ttatagtagc
     5221 atcaataata tttttaatag tattacttgt ggggtgtcaa agtactaaag acacagaaat
     5281 caatgtactc gccacaagtg atttacattc aattattcca gataatttaa tttcctatgt
     5341 agaagaggag agaaaatatg atgagaatct acttatggta gatgcaggag atttttttga
     5401 catgcaaagt ggtgagatga ataagtggtt tactggtcaa aaattagtaa gatttaaaga
     5461 tgggatacca gagtacaaaa aaatagctga accacgtgaa ggagaagttc cattggctaa
     5521 aaaaatggca aagctgaagt atgatgcagt tacatttggg aatcatgaat ttgtatctaa
     5581 tgacaagcag agccttgata aattggtttc tgattttaaa aataacaata taccattagt
     5641 atcagcaaac gtatatgaac aatctggaga aaactacgta aagccatatg taatgaaaaa
     5701 tataaatact gacaagggaa ctgtaaaggt tggtatactt ggtctgacta taaaagaagt
     5761 aggggaacgt tcaaggttta aaagttttga acaagataaa aaggctagag aacttgggga
     5821 acaacctcaa tacaaaggga aattatatgc aaatgattta gtagaagatg caaataagtg
     5881 ggtaaatgtt atggaaaaaa aagaaaagcc agatataata gttgccattg tacattcagg
     5941 tgaagagcca aaaaatccta aaaatccagg aaatagaata aaagaactag caacaactgt
     6001 agagggaata gatgctattg tagcaggtca tacacatgaa aaaatagaac agcatacata
     6061 taaaaataat tctggtgaag aagtaatagt aacacagcct ggagctcatg gtaatagtat
     6121 atcaaagatt aattttaagt tggataagaa gaatgataag tggtttataa aagacaaata
     6181 tagtgaatta aaggtttttg gaaaggaagt aaatataatt acaacttctg gtctgaatcc
     6241 aaaattttca gatgaaatgg cagttaattt gtttaatgaa agagatagag atgttcaacc
     6301 tatctttata gatactggag attttttaaa tactaataca aaagaaatga ctaagtgggc
     6361 taaagagtgg caatacaatg aaaataaagg catctataaa aaaataaatg aatggccaat
     6421 tgtatatctt ggttatgatg ctgtagtact tggtagccat gaatttacag aggataaaga
     6481 gacttttgag gaaaaaaaat ctacattgga ttatattata gaaaactttg aagaaatgaa
     6541 gataccagta ttatcagcta atatatatga tgaatctgga gaaaattatg taaaacctta
     6601 tataattcat aatgtagaaa ctagggaagg aaatataaag gttggagtat taggtcttac
     6661 tactactgat aaggatgaag aagacttaag taactcaagc ttaagcaaag actcaaagac
     6721 ttcgaaaatt cctgaagaaa caggaaagtt atatactaat aatctagtaa aagatgcaag
     6781 cgaatgggta aaagttatgc aaaaggaaag tcctgatgtg attgttgcag ttgtacattc
     6841 agataataat agtactctaa aaaatccaag tagtgaagtc aaaaaacttg caatgtcagt
     6901 agatggaata gatgctattg ttgtaggtca tacgaaaaat aaggtagaag aacatattta
     6961 caaaaataag tctggggatg aggttatagt aacacaatct ggagaagatt gttattcaaa
     7021 aataagtctt gaactaagaa aagaaaatgg caagtggaat atcataaaca aatatagtaa
     7081 agcaataaaa atgaagtgaa tttattgata tataaaccat
//
LOCUS       DAYAOX010000190         7120 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.191, whole genome shotgun sequence.
ACCESSION   DAYAOX010000190 DAYAOX010000000
VERSION     DAYAOX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.191"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(158..535)
                     /locus_tag="ACGI57_002996"
     CDS             complement(158..535)
                     /locus_tag="ACGI57_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9718059.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            complement(722..1693)
                     /locus_tag="ACGI57_002997"
     CDS             complement(722..1693)
                     /locus_tag="ACGI57_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9718060.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            complement(1727..1924)
                     /locus_tag="ACGI57_002998"
     CDS             complement(1727..1924)
                     /locus_tag="ACGI57_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HFG9718061.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILSLYIKQLGVTDVSLI
                     QQYSGIVFGCTFVVAAFFSPI"
     gene            complement(1992..3203)
                     /locus_tag="ACGI57_002999"
     CDS             complement(1992..3203)
                     /locus_tag="ACGI57_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFG9718062.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            3484..4086
                     /locus_tag="ACGI57_003000"
     CDS             3484..4086
                     /locus_tag="ACGI57_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9718063.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPTLWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            complement(4342..5313)
                     /locus_tag="ACGI57_003001"
     CDS             complement(4342..5313)
                     /locus_tag="ACGI57_003001"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HFG9718064.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(5339..6820)
                     /locus_tag="ACGI57_003002"
     CDS             complement(5339..6820)
                     /locus_tag="ACGI57_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFG9718065.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGAIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
BASE COUNT         2373 a         1144 c          765 g         2838 t
ORIGIN      
        1 taacttgata ctccctattc caattatttt aggcatttta ctaattagag aacctattta
       61 atattacttt atgaatagtg agtagctgat taaataatac catttatggt tttcagccat
      121 agattaatta aattcttaga gttctatcct gatataatta ctcttcttct aaatttttaa
      181 aatgactttc aaataaatcc aacatatctt tatcaacaac ttcatcatca tgtagtgctg
      241 aaattaaact ctgtaaagaa ttgtcgtgta tattgcttaa aaaatctttt gtctcaacac
      301 ttaaatactc cttatgtcca acaactattg tataatgagt gtatctatct attttttctg
      361 cacttaaaaa atgtttttta acaagtcttg ataaaagtgt caatgtagtt gtttgtttcc
      421 aaccatattt tcgttccata gcttcaatag tttcttttga tgttactgta tcattttttt
      481 cccaaataaa cttcataact tttaattctg cttgtggtat ttttgaaatt gtcataacct
      541 tacgcccctc tcaaactttt attaaattaa tactatcgca atatagtttt taagtcaaag
      601 attttgtcga taataatatt ttccaatttt taccataggg ttatttttta tataattcta
      661 tgataaatta cataaaaaat atttatttta catcataaaa ttagaaatgt aaatagtatg
      721 attatttagt cttatcttta ataaacaatc cagtaaattt atacatccaa agtgcattca
      781 taaacaacaa taaacttgtt gaaaaaaata ctgttctaat acctaaatgt gctgatattt
      841 gtccccctaa aaatgctcct gatgaacagc ctatatactg ggcagatgca ttatatccaa
      901 atatttttcc cacatattct tctggtgcaa tttttttaag caagctgtta acagaaggat
      961 taagccctgc aactgacaac cctaataaaa atctcaacaa catcagttgc catgcagttt
     1021 ttacaaaagc ttgtggtata aatataatcc cagcccataa aagtgaaatt aacaatactt
     1081 tttgaggacc tattttatcc gacatttttc caagtttttg agctgctaat aaatttgcta
     1141 atcctgatgc agaaaatgtc aatccagcta ttaaagctac atttgatgca aaatttgtaa
     1201 gttgtgatat atatatggtt acaattggtt ctatagagta taaagctagt tgagtaatga
     1261 atgtggttac aaaaatagat gcaattaaaa atttatttgg caatatattt aattgttctt
     1321 taggtataga aattgattct tttatttctt taggtttaaa attatctact acaaataaaa
     1381 tagagatgat aaatgataca aacaacaatc ctcctgttat aataaatgta cttttaagac
     1441 ctaagattga ttctatatat cctcctactg ttggaccaat taatgagcca gtagttgatg
     1501 cagttgcaag agtacccaaa gcccatcctg aatgattttt atctgtctgt gttgcaataa
     1561 gagtagtaca tgcagtagca taacccgtaa aaactccttg aagtattctt aatcctaata
     1621 actgatagac attttgaaca aacgccatgg taaatataac tattcccatc ccaatacttg
     1681 cacgtaaaag cattgatttt cttccatatt tatcagcagc tttccctcaa attggcgaaa
     1741 agaatgctgc tactacaaag gtacatccaa aaactatacc tgaatactgt tgtattaaac
     1801 taacatcagt tacccccaac tgtttaatat ataatgaaag tataggtgct atttggctca
     1861 tccctataga actaaaaaac atacctatcc aacatacaaa taaatttctt ttccacaatt
     1921 ccatatagaa ttacaccccc tgcattttaa attttttcaa taatactaag tttattaatt
     1981 tatgctattg tttactcaat atatccatct tttcttttat aatattgttt aggaaattca
     2041 taacttgaaa ataatagcgg tgataaagaa taggagtaac ttccagcatt actaattgaa
     2101 atcaaatctc ctatgtttgc ttttttcaat ttaacattat ttgcaattac atctaatgat
     2161 gtacagagat tcccaacaac atttacatac tcttcttctt ttaaattatt taatactgaa
     2221 agagaaaagg catgatttga tgtatataat ggctcttgac cttctaattc tactccacaa
     2281 gtaatatttt caattaaatt agaaataata ggtctcataa atccattaag tccatttttt
     2341 acaataagat attttttccc tcttgatact ttaatatcta caattttaac ataataatta
     2401 cctgatttac aaactatgaa acgtcctgat tcaataagaa gtcttgattt tagtgtgttt
     2461 gaattagtaa aagcaatctt ttgagttaat tttgaaagct ctttaagatt aagtggattt
     2521 tctctaagat tatcataagc tactccaagt ccactaccaa aatttataaa ttcaatatta
     2581 atatacttac ttttatttaa atttaaagct ataaaaaaac atttttcata ataatgtcct
     2641 aacttttcaa aatccaatac ctgagattta atatgtatat gaatcccaac tacagataca
     2701 taatcacaca tacttaacat cttaaaaaca ctgctcatat cttctatatc tataccaaat
     2761 ttgcttgatt tagcactatc atcatccata gtaaaatcag gatttattct tattcctatt
     2821 ttaactttct gattatactt tttagatatc ttatttataa gttctatttc tgtaatacta
     2881 tctgcaataa ttatgcattt atctaaaaca gtttctatat caatctctgt tttgcctggt
     2941 gatgaataat aaatatcatc tttagaaact ccacattctg atgaaatcat aacttcatta
     3001 gttgaagaag catctgctcc aaaaccttgc tctgcaatgc tttttacaat tttgttatat
     3061 ggatttgttt ttatagaata taaaaaatca aaacctaaaa tattattttt caattcctta
     3121 catttttcaa ttatattagt ctcatcatat atataacatg gtgcattttc ttctataaat
     3181 ttatagtctt catttgagta catatttttt acctccaaga ttttaaatat aatcataaat
     3241 taagcaatta taatcctata agataatttt acaataaatt accatagtta actacacaca
     3301 tatatgtaaa aacataccta aaatattttt aattatagat acattccaac ttttattgat
     3361 aaaacaattt gtttcaattt tattacaaat atacaaagtg tattgttgtt aacgtattct
     3421 catagtatcc taaaactata ttaagcacta aaaactattt taaaatactg tggaggtatc
     3481 aatatggaag tatgcattct tatgggaagt cctaagaaaa aaggtaatac tgctgctatt
     3541 ttaaaacctt ttattgagga attagaaaat tataattcaa atattgaagt aatatggtta
     3601 tatgattgta aaatagagcc atgtattgct tgtaaaaaat gtcaggataa cttatttgat
     3661 tttggatgtt attgtaaaga tgatgttcag aaaattttca agaaaattct tatgtgtgat
     3721 ttaattgttt ttgctactcc aatttattct tggtactgca cttctccaat gaaagcacta
     3781 cttgatagat tggtgtatgg aatgaataaa tactatggta atgagaaagg ccccactcta
     3841 tgggcaggaa aaccagtagc acttattaca acatgtggat atagacctga aaagggtgct
     3901 gatatttggg aaaatggtat gaagcgctac tgtaagcatt cacagttaaa atatatagga
     3961 atgcttgttg aacgtgattt aggtaaacct aattttatga ataaagaaaa agaagaacat
     4021 tctcgtcaat ttgcaaaaca aatttattat attttagata attcaattga agtagataac
     4081 tgctaaagac ataatactcc aagtaaagta ttgataatat aaataaatct tacataattt
     4141 gcttatttaa aataagatat atattcttct aaaaagtaac aataattaaa atatttttta
     4201 tcatttcaac aatgataaag aggcttatct ctattttgag acagcctctt tattgctttt
     4261 tatatgttaa ttattttcat agtaaacatc cttaaaaact ctttacatgt tacttccttc
     4321 aaaacctatt actcattcct actataaatt ttttataaat tcagttattt cttcctttgt
     4381 tgcatatgaa cttatcgcac ctttaatagt tgtactacga gctgcataat aattagaaaa
     4441 ttctaaatac tcatttaacg tttcttgttt catttctttt atatcattca atgttatttg
     4501 tttatacaat aaggtgtata aaaatgaacc tatataagaa tctcctgctc cggttgtatc
     4561 tattgcattt actttaaccc ctttacacat accttttact tttttagtgt atgcatctac
     4621 tccatctttt ccttttgtat ataatactag ttcaacattc cccacaaaga gtttatgtaa
     4681 tgatttttct atatcatttt ctcctgttat aaattctaat tcttcatcag atatcttaac
     4741 aatatgagca aaaggtaaaa attctgatat tgccttttta caagattgct cactatccca
     4801 taaaggtaat cttatattag ggtcaaagct tattatacta ttactttcaa gtgcgtattc
     4861 tattgctttt ttatgtgcta atttcattgg actatctatt aaatctactg aacaaaaatg
     4921 taggatatga caattattaa accactcttt tttaacttca tcagcttcta aaagcatatc
     4981 tgcacttgga tttctataaa atgagaaatc acgattacca tcttctttta atgatacaaa
     5041 agcaagtcct gtatttgctt tacttgttct caatacataa tcagtattta catttacttc
     5101 atttaataca tctattatat aatcaccaaa tgcatctttt cctaacttag atataaaact
     5161 cgattttcct ccaagctttg ctacaacagc actaacattt gctggagctc ctcctgctac
     5221 tcttctaaat tctgatacat tttttaattt accaccattt tgattcggta taaagtctat
     5281 taaagcttct cctatgctaa ttactttttt catcttttct accccttata atgtgttttt
     5341 ataattcaat accttgcttt aatttaaatt tagtatactc atactctact attttatcac
     5401 tataaaccct tatttgatta gaattttctt tagcaaatat tctactagta aatactactt
     5461 ctccttcatt tataaaaatt tctaaaatac ttctatccgc tagtactttg atattagtat
     5521 tttcatcaat atttatatat tcttttctat ttgttccata ttcaacaccg aaaacttttt
     5581 taaagttgct tctgtctaaa gttacaatgt ttcctaattt gttaaattct aattttacac
     5641 cttcatcttg tgatgaaaat aattccagcc caaatttatt gctgtcaata ttttttatat
     5701 taatattaag ctcatataca ttttcatcat tttctataat attaatatta ttctttatag
     5761 tattttgccc acttgtttca tcatatctta atttaattaa ttcatctact ggcatttgca
     5821 atagcttatt attttttata tttaattttc ttggaacagt tagacaatgt gcccatttgt
     5881 ttttatctgt agggtataaa acttctccca ttccagccca tgcaaatagt attatttgtg
     5941 agtcctttat aaatgtttgt ggagcataga aatcaaatcc tttttccagt tctttcattg
     6001 tatctatttc aaatgttaaa ttatctaaat ccatattacc aatagcatat actacattat
     6061 atatattttg ataatcaaat cctttaggtt ctactccttg tggtgaaaat attaatatat
     6121 ctttttcatc tatattaata taatctggac attcccacat gtaaccaaaa tcttcatcta
     6181 tattttttac atttaattct cctttaaaat tccaatctat agagttttta gattcatata
     6241 ctattatagc tcctttttta tcacttgttt gtgctccaag taacataaag tatttatcct
     6301 ttcttttaaa tacttttggg tctcttacat gtcctgtata tccttttggt atttcactta
     6361 ctattggatt attttcatat ttagttattt taccatcctt gttcattata gctaagtttt
     6421 gatatgcata tctatcttct gcactatatt ttatattccc tgtataatac atatgcaatt
     6481 ttccatctac ctctatcgca ttacctgaat atgctccatg agattcataa ctttcgattg
     6541 gtattaatgc tacatcttct ctattccagt ttacaaaatc atcagaactt acataagccc
     6601 aatgcttcat tccatgagtt gcatcatatg gataccattg ataaaataca tgatacttac
     6661 cattataata tgctaatcca tttgggtcat taagtagtcc atgttgtgga tgtatatgaa
     6721 atagtggttt atatttatca tttaaagata tatttcttaa tttatttaat tcttcttttg
     6781 tcgattctaa tatggttcta tatttaggct ctttgtacat atttttattc cttttctttt
     6841 ataattttat atttgcttta ttattgctat ctaaaacaat tcttagtctt attacacaat
     6901 gtgctacatc ttttatattt tgtttattcc tatgtactct aataattact ttgatacttt
     6961 gttacaatca gttatttttg cactcatttt gctattctat cggtttaatt aaaccggttt
     7021 cgaaaccggt ttaattaaag attagcataa atttatttca gatgcaatat ttctttcaaa
     7081 aaagttttcc taatttttag aattatagtt tttttagtaa
//
LOCUS       DAYAOX010000191         7106 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.192, whole genome shotgun sequence.
ACCESSION   DAYAOX010000191 DAYAOX010000000
VERSION     DAYAOX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.192"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            30..410
                     /locus_tag="ACGI57_003003"
     CDS             30..410
                     /locus_tag="ACGI57_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9718066.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            535..735
                     /locus_tag="ACGI57_003004"
     CDS             535..735
                     /locus_tag="ACGI57_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718067.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            931..1659
                     /locus_tag="ACGI57_003005"
     CDS             931..1659
                     /locus_tag="ACGI57_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718068.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            1681..2598
                     /locus_tag="ACGI57_003006"
     CDS             1681..2598
                     /locus_tag="ACGI57_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HFG9718069.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            2740..3924
                     /locus_tag="ACGI57_003007"
     CDS             2740..3924
                     /locus_tag="ACGI57_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9718070.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTYLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            4031..4219
                     /locus_tag="ACGI57_003008"
     CDS             4031..4219
                     /locus_tag="ACGI57_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFG9718071.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            4368..5018
                     /locus_tag="ACGI57_003009"
     CDS             4368..5018
                     /locus_tag="ACGI57_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9718072.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            5121..6059
                     /locus_tag="ACGI57_003010"
     CDS             5121..6059
                     /locus_tag="ACGI57_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFG9718073.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            6181..6936
                     /locus_tag="ACGI57_003011"
     CDS             6181..6936
                     /locus_tag="ACGI57_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9718074.1"
                     /translation="MKKYNISENGALIKNDSIESKSESVKVVQISDTQIGSFYSTKNL
                     KKVANKINTLNPDIIVFTGDLIDYSNKNPSVDEITTILSSMNARLGKFSVFGNHDYMY
                     KLPRYYRQIMKNSNFNLLVNENKKIKLKDDKYINILGVDEILNGNPNIKYLESQIDNK
                     SFNLLLAHEPDLVDMLSKDTMNLVLSGHSHGGQIRLPIKGALVTPPYGRKYTKGFYDI
                     NGNHLYVSSGLGSTKLPFRFFNIPEIIEFNIII"
BASE COUNT         2939 a          668 c         1273 g         2226 t
ORIGIN      
        1 tttatttaat aataaaggtg ggtattgtta tgataaataa aataggaaaa ataactctct
       61 atgtaaataa tcaagaggat gcaaagttat tttggacaga aaaattgaac tttgtagtaa
      121 cttatgaaaa ggaaatgggt ccaaatatga aatgggtgga agtagcacca agtaaaaatg
      181 aatttacaac atttgtgttg tatgataaaa atctaatgat gaagcaaaac aaagagataa
      241 atgtatctca tccatcaata attttaagta caagagatat taatggaact tatgaacaaa
      301 tggcaaataa cgaggttgag gttggggaat taatggagat gccatatgga aggatgtttt
      361 cttttaaaga ccaagatgga aatgattatt tggtcagaga ggataaataa ttttacaaag
      421 tattaaattt actcaatatc tgatataatt agagacaata attattataa actagtctag
      481 gattagaatt atataattaa aaattaagtg taaattttga gtagaggtga taaaatgttt
      541 aagaaaatgg cagtactaaa agatatagca actaaaatag gtcgtaaaaa agcgtatgaa
      601 ctattagaaa tggttgaagg taatgatgcc tttgtagctg aggtaaagat aaaaaagaat
      661 ggaatagaat ctaaaaaaga agaaattatg ttaaaagata atcaaaaaat aatattagag
      721 tatatagaag gttaaatttt aaatacatat aagttgatat actaaatttc tgagatgagt
      781 ttacataaaa ctcatctttt ttacttttca ataactttgt tttattattt aatatacatt
      841 ttatctcctt agagttgctg atatataaaa attactgata taataaaaga atggatatta
      901 atgtaatatt ttatagaaaa gaggggtatt atgcttgaaa taaaaaatct ttcctttaat
      961 gttgagtcta acaatgagga actaggaata ataaatgatg ttagcctttc atttgagagg
     1021 ggaaaactta ttgttatcac aggtccaaat ggtggaggaa aatctacaat tgctaagctt
     1081 attatgggaa ttgaaaaagc tacgagtggt cagataattt tagatggaga agatataaca
     1141 aatcttagta ttacagaaag agcaaaaaaa ggaataggat acgcatttca acaaccacca
     1201 agaataaaag gtatgacagt tgaaaattta cttacacttg ctcatggaaa accattgtct
     1261 actgatgtat gttgccaata ccttacagat gttggattgt gttcaaaaga ctatttaaat
     1321 cgtgaagttg ataactctct ttctggaggg gaaatgaaaa gaattgagat agcaactctt
     1381 tttgctagag atttaaaggt ttctatattt gatgaacctg aagcagggat agacctttgg
     1441 agctttggaa aactcaatga aagttttaag aaaatacatg aagagtctaa tcagactata
     1501 atcataattt ctcaccaaga aagaatatta gagttagctg atgaaataat tgttctacaa
     1561 gatggttcag taaaaagcca tggaacaaaa gaggctatat tacctgaaat catgtgtcaa
     1621 gttaattcaa gttgtgaatt aatgaaagac atgaattaat tagaaaggaa ggcaaattaa
     1681 atgaacaaaa tagatttaaa tcttttagaa aaagttgctg aacttcgtgg aactccatta
     1741 ggagcataca atatacgtaa aaatgggcaa ggagtagaga gacattcaac tgaaaatgta
     1801 actataattc ctaaaactga taaacctgga atagatatta tagtaaagcc aaatacaaaa
     1861 ggagaaaatg tacatatacc agtaatagta acagaggctg gtgtacagga tatggtatat
     1921 aatgattttg aaattggaga aaatgcagat gttgttataa ttgctgggtg tggtatacat
     1981 aactgtggtt gtgatgatag tgaacataat ggtatacata gatttttctt acgtaaagga
     2041 gctcgactaa aatatgtaga aaaacattat ggtgaaggtg atggagaagg aaagagaatt
     2101 ttaaatccag ttactattgt caatatggat gaggattcat attgtgaaat ggaaacaatt
     2161 caaattaagg gtgtagattc aactaaaaga gaaatggatg caaacctagg taaaggagct
     2221 aaaatagttg tatttgagaa attattaact catggtaatc aagttgcaga atctaatatg
     2281 aaggttatct tagagggtga aaactcttct gctcaagtag tttctcgtac agttgggcaa
     2341 gataattcta agcaaaagtt taatccttgt gttattgggg attccctttg ttctgcacat
     2401 gttcaatgtg attcaataat tatgggaaat tctcaaatta gtgctatacc tgaaattgct
     2461 gcaaatcatc cagatgctct tttagtacat gaagcagcta ttggaaagat tgcaggagac
     2521 caaatactaa agatgatgac attaggtctt actgaagaag aagccgagca acaaatactt
     2581 aattgcttct taaagtaaat taaagatttt atttaattta atataattaa tatagtataa
     2641 tatagtgaat aagtctaaaa tatttttatt tttaggctta ttttctttat gtaagaaaag
     2701 ctattagaga aattatattg taattaggat gtggaataaa tgaaaaattt acttataaga
     2761 aatgggacaa taatatctcc aacagatggt atcaaatttg aaaaaagaga tatcttggtt
     2821 ttacatggga aaatatatgc tattggattg aaccttaaag agaaaataag aaaagaaaaa
     2881 ttacttacaa ataaagagtt cgagataata aatgccgata atatgtatat ttccccagga
     2941 tttatagatg ttcacaccca ttgttataag agaaaattac ctacaggtat ggattctgat
     3001 tcaattggaa ttgaaaaagg ttctacagtt atatttgatg caggtacagc tggtccctca
     3061 aactactatg attttaaaaa aaagtatatg gatgagtgta aaacagaagt atattctttt
     3121 ttaaatgtta caaatgaggg actaaatata ttgaatgaat caacttattt agatgttata
     3181 aattttgaca aggttgagtc aattgtagaa aaaaatatag aaaagataaa gggtataaaa
     3241 gtaaaagcga gtaaatttca atctaatgaa agtggtatag atataattaa gaaatcaaag
     3301 caagttgcta aaaaattaaa tttaccctta gtggtatata taggagagta tccatcatat
     3361 atagatgaag taatgaatat tttgggaaaa ggagatgtgg ttacaccggt atatggaaat
     3421 agtacaaatg gaatccttaa agaatctggc acattaagaa aatcagtaat aaatgcaaag
     3481 aatagaggag tattgtttga tgtatgtcat ggagtggacc aatttgattt taaagttttt
     3541 aaaaaagcaa taaagcaagg tttagagcct gacctaataa gtactgatat gcatataaaa
     3601 aacatgaaag acgtaaacta ttctctttta aatgttataa ataaaattat ggaacttggg
     3661 atatccctag aaaagtgtat agaaaaagta actgaatacc caacaaagat tttcgattta
     3721 catggaaata aaggcagaat aaagattggt ggagaaggtg attttacaat atttacaatg
     3781 gaggagtgta atgatattat aaaagactac aataataatg aacttgtttt aaataaaaaa
     3841 ttaagattgc aatatactgt aaaatcatgg atgaaaagca gtgaagttta tagacatgga
     3901 tacgaaaata ctataataaa ctaaacaata gtgataaaat aataaatata tgtttaaatt
     3961 ggtatatatt ggataaatat ctaaggaaac aacaattaaa aaataataat tttaggggag
     4021 gtattttagt atgaaagcat ttgttgataa ggatgtatgt gtagcttgtg gagcatgtat
     4081 aggaacatgt ccagacgttt ttagtatgag cgatgaaggg cattcagtag caattgaagg
     4141 agaaatacca gcagatttac aagatttatg taaagaagcc gaagaaggat gtccagtttc
     4201 agctataaca gttaaataga gctgtttaaa tttattttta aaataaatat ataagattta
     4261 attggtactt aagctatagt attagaaatt tctattacta tagcttttta aaatagataa
     4321 acttaatata taagatatta ctttttacaa atatggagga ctgaaatatg aaaaaaaatt
     4381 atgaaatagt attattcgat ttagatggaa cacttacaga ccctaaagaa ggaataacaa
     4441 agtctattca gtattcttta aatagctttg gcataaaaga agatttggaa aacttagacc
     4501 aatttatagg accaccatta catgatactt tcaaggagta ttataaattt gaagataaaa
     4561 aagctaaaga agctgttgaa aagtatagag aatattttgc tgataaaggg atatttgaaa
     4621 ataaaattta tgagaatatg aaagagatat tagaaatgct ctataaaaat ggtaaaatat
     4681 tattagtagc aacttctaag ccaacagttt ttgcagaaac tatacttaga tattttgata
     4741 tagatagata ttttaaatat atagcaggta gcaatcttga tggtacaaga gtgaataaaa
     4801 atgaagttat acaatatgta ctagacctat gtaatgtaaa agataaagat aaagttatta
     4861 tggttggaga tagaaagtat gacataattg gagctaaaaa aataggtata gattctattg
     4921 gagtattgta tgggtatggt tcttttgaag agattagtga aagtgaacct acttatattg
     4981 tcgaaaatgt agagtctata aaagatattt tgttataaat atcttttata gacaaaaata
     5041 ttacaaaatg gtgtcgcata tttaaaaata tgatattata gttaaggaag tcatttaaaa
     5101 aagaggagga tgcacttaaa atgaaatcta ttttaattat gggtggaagc gacttcatag
     5161 gttctgcact tgctaagcgc ttgattaaat gtgggtatca aattgatata cttacaaatg
     5221 gtaaaaagga aatagattat aatggtttta aagaacatct aatttgtgat agaaaagttc
     5281 gaaaagatat ggaaaacatt atcacaggaa gaaaatatga ttacatatat gatatgacag
     5341 catatacaaa agaagatgta agtaatctga ttgattttat aagcatggat aacttaaaaa
     5401 agtatatagt attgtcagca ggagcagtat ataaagatag tggaagaaat ataaaagaag
     5461 aaaatgagaa aggtgaaaat gaaaactggg gaaaatatgg gttaaataaa aaagaggctg
     5521 aagattttat tataaatagc cctataccat atataattat tagaccaact tacatatatg
     5581 gtgaaaataa taatttatat agagagtatt atttctttga aaaaatagaa aaaaatgaaa
     5641 agataccagt accaaaagga aaacaagtta gtaatcaatt tatatatatt ggggatttgg
     5701 taaaggtatt ggaaagcata atgaaaaatc ctcatgtaag agaggcctat aatgtgacaa
     5761 atccacagtt aatttcttgg gatgacctta tatatacatg tggagaaatt attggcaaag
     5821 aacctataat aaaatatgta gatatggaaa aagtagagtt tagagaaaga acgtatttcc
     5881 catttagaaa tatagacttt aatcttgata taaataagct tattgaacat gggctttata
     5941 taccaaacgt gttactaaaa gaaggtctta cagcaacata taaatggttt tcagcaaata
     6001 agccaaaaat gcatgacaga aaaatgaaca aagttgagtc tgtaatgcaa atagtttaaa
     6061 aaaataaata agtaggtaga tattttgaac tcaaaaatta agtatttttt aaaatttttt
     6121 atcttaatat ttattttgat ttttatatac tcagttgcga tagagccgag tctattaatt
     6181 gtgaaaaaat ataatataag tgaaaatgga gcattgataa aaaatgattc aattgagagt
     6241 aaatctgaaa gtgtaaaagt agttcaaatt tcagatactc aaataggtag tttttattca
     6301 actaagaatc tcaaaaaagt agctaataag attaacactt tgaatccaga tataatagta
     6361 tttactggtg atttaattga ttactccaat aaaaatccaa gtgtagatga aattacaact
     6421 attttatcaa gtatgaatgc taggttggga aaattttcag tatttggcaa tcatgattat
     6481 atgtataaac ttcctaggta ttataggcag attatgaaaa attccaactt caatctttta
     6541 gtaaatgaaa ataaaaaaat aaaattaaaa gatgataaat atattaatat attaggtgtt
     6601 gatgaaatat taaatggaaa tcctaatata aaatatctgg agagtcaaat agataataaa
     6661 agttttaatt tattgttagc acatgaacca gatttagtag atatgcttag taaagataca
     6721 atgaatttag ttctatctgg gcatagtcat ggtggtcaaa taagattacc tattaaggga
     6781 gcattagtta ctcctccata tggaaggaaa tatactaaag ggttttatga cataaatgga
     6841 aatcatttat atgtgagttc gggtcttgga agtacaaagt taccatttag attttttaat
     6901 ataccagaga taatagaatt taatattata atctaacata aaatatttag aaaatataat
     6961 tttgcttttt ataatatgta gatgtatata ctgtaaaaat ggattaaacc agtagattga
     7021 atgaattaat agattaaatt aatatataaa atatttaagt tataattaat gtattagaca
     7081 aaataaatac tattaagaac tattaa
//
LOCUS       DAYAOX010000192         7085 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.193, whole genome shotgun sequence.
ACCESSION   DAYAOX010000192 DAYAOX010000000
VERSION     DAYAOX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.193"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            2..75
                     /locus_tag="ACGI57_003012"
     tRNA            2..75
                     /locus_tag="ACGI57_003012"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Met,seq:cat)
     gene            90..166
                     /locus_tag="ACGI57_003013"
     tRNA            90..166
                     /locus_tag="ACGI57_003013"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Ile,seq:cat)
     gene            184..260
                     /locus_tag="ACGI57_003014"
     tRNA            184..260
                     /locus_tag="ACGI57_003014"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:218..220,aa:His,seq:gtg)
     gene            269..344
                     /locus_tag="ACGI57_003015"
     tRNA            269..344
                     /locus_tag="ACGI57_003015"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Lys,seq:ttt)
     gene            352..425
                     /locus_tag="ACGI57_003016"
     tRNA            352..425
                     /locus_tag="ACGI57_003016"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:384..386,aa:Cys,seq:gca)
     gene            433..509
                     /locus_tag="ACGI57_003017"
     tRNA            433..509
                     /locus_tag="ACGI57_003017"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:467..469,aa:Arg,seq:acg)
     gene            522..597
                     /locus_tag="ACGI57_003018"
     tRNA            522..597
                     /locus_tag="ACGI57_003018"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:555..557,aa:Val,seq:tac)
     gene            673..1086
                     /locus_tag="ACGI57_003019"
     CDS             673..1086
                     /locus_tag="ACGI57_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718075.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1395..4826
                     /locus_tag="ACGI57_003020"
     CDS             1395..4826
                     /locus_tag="ACGI57_003020"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFG9718076.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTRESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5068..6996
                     /locus_tag="ACGI57_003021"
     CDS             5068..6996
                     /locus_tag="ACGI57_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFG9718077.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         2901 a          810 c         1370 g         2004 t
ORIGIN      
        1 tggcggtata gcttagttgg ctagagcgtt cggttcatac ccgaaaggtc ataggttcga
       61 ctcctattac cgctactgaa atttaatgtg ggccattagc tcagttggtt agagcgcccg
      121 gctcataacc ggtaggtctg gggttcgagt ccctgatggc ccaccacatt aaattaactt
      181 atggtgggta tagctcagtt ggttagagcg ccagattgtg gctctggagg tcgtgagttc
      241 gactctcatt atccacccca acttatatgg tctattagct cagtcggtag agcacctgac
      301 ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc accaaaaagg cggcgacata
      361 gccaagtggt aaggcagtgg actgcaactc cttgatcccc agttcgaatc tgggtgtcgc
      421 ctccaataaa atgcgctcat agctcaactg gatagagtgt ctgactacga atcagaaggt
      481 taggggttcg agtccctttg ggcgcaccat atgaatagcg agggattata gctcagctgg
      541 gagagcacct gccttacaag cagggggtca caggttcgag ccctgttaat cccaccagtg
      601 caaacctcta gtaaaactac tagaggtttt atttttgatt gaaaaagttt ttatatttag
      661 gaggtaaaaa atttgtatat agtaatagga aatgaaatag ttgatagtga agaacttaaa
      721 ataattatag ataaaaatag tgaatttaaa gttgaaaaag atttaagtaa aagcacaaaa
      781 agagaagatg taatagcata tcagttaagt atagatttaa attacctaga ttctcttata
      841 aatgagcaat gtaatttagc cagtctatct gatgaagaga aatttgatga atatatgact
      901 ttgtctgatg aacttgcact ggatttagaa gagttaatgc caaaatatac aataataaat
      961 gctagagcat ataaattaga cgaggtagat ggaattgtaa aaataatact agctgtagcg
     1021 tatgcagatt tagggcactt gaaactttct gatgtagtaa agaggctatc tagacaagta
     1081 gattaggata tgaatatgaa ctatattaaa aaaatgtata aaatcttacg taaagatgaa
     1141 aataattatg taaacataga aaaaaacagt ataaaatcaa gtcaattgta gaaattttca
     1201 cataaattca tctgaaattc tataaaaaat gccttaattt taatgaggta aatcgagttt
     1261 acacagtcta aaaattactt atatttataa atttatctta ttaattaagg aactttctta
     1321 aacatcttca aaaataaagt attttagtct ataatcttta atattacaca aacaataaag
     1381 gagaagaata gagtatgctt aaaaagttta ataaaatact agtggctaat agaggcgaaa
     1441 tagctataag aatatttaga gcctgttcag aattggggat aaaaagtgtt ggtatataca
     1501 gtaaggaaga taagtatgga ttgtttagaa caaaagcgga tgaatcatat ttgataggag
     1561 aaggtaaagg gccaatagat gcatatttag atatggatgg aataatagac ctagcaaaga
     1621 gaaaaaaagt tgatgctata catccaggat atggttttct agcggagaat gcagaatttg
     1681 ctcgtaaatg tgaagagaat ggaattactt ttataggacc atcatctaag gtaatgaata
     1741 tgatggggga taagattaac tctaaaaaaa tcgcaaaaga agttaatgtt caaactatac
     1801 caggagtaga aaaagcaata agaagtactg aagaagcaaa ggaagttgca aacaaaatag
     1861 gatatcctgt tatgataaaa gcatctaatg gcggtggcgg tagaggtatg agaattgtac
     1921 atcgcgaaga agacttagag cttgaatatg aaactgcttg tagtgaatca agaaaagctt
     1981 ttggagaaga tattatattt atagaaaaat atatagctga tccaaaacat atagaagttc
     2041 aaatattagg tgataattat ggaaatatag ttcacttata tgaaagagat tgttcagttc
     2101 aaagaagaca tcaaaaaata atagaatatg caccagcttt ttcattagat gataaagtaa
     2161 gaaaagagat atgtgaagat gcagttaagt tatcaaaaca tgtgggttat tctaatgcgg
     2221 gaacattaga atttttagta gatgcaaatg gtgggcatta ttttatagag atgaacacaa
     2281 gagtacaggt tgaacatact gtaacagaga tggttacagg aatagatata gttcaaagtc
     2341 aaatactaat agcccaaggt tacagcttag atagtgaaga aattaatata aaatcacaag
     2401 atgatgtgga aataagaggt tactctatcc aatgtagaat aactacagaa gaccctaaga
     2461 ataaattcat gcctgataca ggtaaaattc aagtttatag aactggttca ggatttggta
     2521 taagactaga tggaggaaat ggatttacag gggcaaacat aagtcctcat tatgatagtt
     2581 tattggttaa gacaatatct tgggatagaa cattccaagg agcaataaat aaaactataa
     2641 gatctataaa agaacttaga gttagaggtg ttaaaactaa tgtaggattc ctagttaatg
     2701 ttttaaataa tcctatattc tcaaatggta aatgtagtac taagtttata gatgaaaatc
     2761 cagatttatt tgaaataaca gaaagtaaag acagaggaac aaaattactt caatttatag
     2821 gagatgtaat tgtaaatgat aatgcttgta aagaaaaacc attatttgat gcattacatg
     2881 acccaagaat ggataaggat ggttcaaaat cagaaggaag taaaatactt ttcgataagt
     2941 taggtaaatc tgcatatata gaaaagataa aaaatgataa gaaattacta ttaacagata
     3001 caacaatgag agatgcacat caatcattat tagcaacaag aataagaaca tatgacttat
     3061 taaaagctgc taagccaaca gaaaaatatc aaaaggattt attctcactt gaaatgtggg
     3121 gaggagctac ttatgatgtt gcatatagat ttttaaaaga atctccttgg cgtagattac
     3181 aaaagctaag agaagaaata ccaagcataa tgttccaaat gttgcttaga gcatcaaatg
     3241 gagttggata taaaaactat ccagataatg taatagaaga atttacaaga gaatctgcta
     3301 gacaaggtat agatgtattt agaatatttg attcattaaa ctgggttgaa aatatgaaac
     3361 catctattaa tacagcttta gaaactggaa aaattgttga agcaactatg tgttatacag
     3421 gtgatatact agataagact aaaactaaat acaatctaga atattatatt aaaatggcac
     3481 aagaattaga aagcttggga gcagatataa tagcaataaa agatatgtca ggtcttctta
     3541 aaccatattc agcttacaca ttagttaaag agttaaagaa aaatgtaaaa gcaccaatac
     3601 atttacacac tcatgataca agtggaaatg gtgtagcaac ttgtttaatg gcgtcagaag
     3661 ctggagtaga cataattgat gctgcattag aatctatggc aggacttaca agtcagccat
     3721 cacttaatgc tatagtagag gcacttaaga atacagagag agatacagga atagatttat
     3781 ttggatatga tgagttagga aaatattata aggatttaag aaaagtttat aataaatttg
     3841 aaagtgattt aactaattct tgtgcagaaa tatataattt tgaaatacca ggaggccaat
     3901 atacaaactt aaaacctcaa gctgatagtt taggattagt taatagattt gatgaagtta
     3961 aagaaaaata taaagaagct aatgaagttg taggagatat aataaaagta actccatctt
     4021 caaaagttgt aggagactta gctatcttta tgactaaaaa taaattagat aaagacaata
     4081 taatagaaga aggtaaaaat ttatcattcc cagattctgt tgtggattac tgtaaaggta
     4141 tgattggtca accagaaggt ggaataccaa aagatttaca agaggtagtt ctaaaaggtg
     4201 aagaagctat aacagtaaga ccaggttcat tattaccagc agaagatttt gatgaaatag
     4261 ctaaatatct aaatgaaaag tatgatataa atgctaatat aagaaatgtt ataagttatg
     4321 ctttatatcc aaaagtatat gaagattata tcaaacatct acaacattat aatgatatat
     4381 ctaaacttga aagtgatgta ttcttctatg gattaaataa aaatgaagaa tgtgaagtag
     4441 aaatagaaga aggtaaggta ttaacaataa gattagttga aattggtgaa gttaaggaaa
     4501 atggatttag aactatagga tttgagttaa atggaatggt tagagaagta gaaataaaag
     4561 ataagaattt ctctggaaaa attaataatg ttgaaaaagc tgatatgaat gaccctcttc
     4621 aaattggagc aagcatacca ggaaaagtta taaaaataat ggttaaagaa gaagatgaag
     4681 taaaagcaaa tcaaccatta atcgttatag aggctatgaa gatggaaact ataatagttg
     4741 ctaagactga tggagtaatt aagtcaatca aagtaaaaga agatgatatg gttgaagata
     4801 aacaattatt aatgataatg aagtaattta attaaaataa attaaaaagc actctgaaat
     4861 gtttaataaa aaatgtttta gagtgttttt atttatttta attaaatatt tgttaaaaaa
     4921 aagaggacaa ttacctccag atgtagaaat aaagctagta aggaaaatgt tatctaaaaa
     4981 ttctaaatat agtaaatatt ttccataata tttcttagga aataacagca aagataaaat
     5041 acataaattt aaaaggtggg tgcttatatg aaggtttatg atagtaaaat gttgagaaat
     5101 gtagcggtat taggacatag cggatgtgga aaaacaaatc taatcgaaac tatagcttac
     5161 acagcaaaca ctaataagat acctaaatta acagacaagg taaatatgac ttatagcatg
     5221 ggattaatac ctatagagta taatgattat aagtttaact tattggatac cccaggatat
     5281 tttgacttta gcggagacgt tgtgtcatct cttagagcaa gtgatgcagc aataatagta
     5341 atagatgcaa cagcgcctat tcaagttggt acagagaaat cattagaact aactgagagt
     5401 attcctaaga taatgtttat aaataaaata gataatgaaa aggcaagata caaagatgca
     5461 atagcaatgt taagagaaaa atacaataat aagatagttc ctatgataag tcctatatac
     5521 aaagataaaa actttgtaaa gttacataat gtttttgaaa acatagatga tttagaaggt
     5581 gaatttaagg aacaagctat gagtgttaaa gaagcattaa tggaacttat agcagaaact
     5641 gatgaccaaa tattagataa atattttaat ggagaagaac taactacaga agagatacaa
     5701 aaaggtatta ttataggtat acaaagaggt gatattatac ctgtaatatg tggttcaact
     5761 ataaataata taggcacaaa ggaaatatta gatacaatat caagttattt agaacctatc
     5821 tttacagagg aaagcaaacc atttagggga ttagtattta aaactatggt tgacccattt
     5881 gttggaaaga tgtcatatat aaaaattact gagggtgtac tttctaagga taaagatgtt
     5941 tttaatataa ataaaaatgt aaaagaaaaa atagctaata tatatacatt gagaaatagt
     6001 gaattagtag aaatagaaaa agctaaagct ggtgatatag tagttataac taaagttaat
     6061 tctctaaaaa caggagacac tatatctgca gataaagatg cagaagcttt agaaaagata
     6121 gattttccta aaccacaaat atattatgca gtaactccta agaataaagg tgatgaagaa
     6181 aaagtagcat ctgtattaaa taaactagta gaagaggacc ctacattaca ttggtataga
     6241 aatacagaga caaaacaagc acttttagga ggtcaaggag aacttcatat aaaaacaatt
     6301 aaaaataaaa tgaaagataa gtttggtgtt gatgttgaat tgaatgacct taaagttcct
     6361 tatagagaaa ctataaaagg aacagctgat gtacaaggaa aacataaaaa gcaatcaggt
     6421 ggtcatggtc aatatggaga cgtaaaaata agatttgaaa gatgtgaatc tgattttgaa
     6481 tttacagaag aaatatttgg tggttctgta ccaaaacaat atataccagc agttgaaaaa
     6541 ggtttaaaag actctatgca aaaaggtata ttagcaggat atcctgttac aaacataaaa
     6601 gctacgcttt atgatggttc ataccatgat gtagactctt ctgaaatggc ctttaaaatg
     6661 gcagcaagtg cagcctttaa aaaaggtatg gaagaagcac acccaatact actagaacct
     6721 ataatgaaac ttaagataac tgttccagaa gaatatatgg gagatgtcat gggtgatata
     6781 aataagagaa gaggaaaaat atttggaatg gaaccagatg acaaagggaa acaaataata
     6841 tttgcagaag ctccacaagc agagacattt aaatatgcaa tagatttaag agcaatgaca
     6901 caaggtagag gatactttga aatggaatta gagagatatg gagaggttcc atcacagttt
     6961 gcagaaaaaa taattgggtt agctactgca aagtaatgaa caaaataaat agaaaaataa
     7021 ttaatatata ttattaaaaa tatttttagg gttttaacta ggtataggtt tatgttaggt
     7081 aaact
//
LOCUS       DAYAOX010000193         6997 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.194, whole genome shotgun sequence.
ACCESSION   DAYAOX010000193 DAYAOX010000000
VERSION     DAYAOX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.194"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            109..300
                     /locus_tag="ACGI57_003022"
     CDS             109..300
                     /locus_tag="ACGI57_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFG9718078.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            455..1864
                     /locus_tag="ACGI57_003023"
     CDS             455..1864
                     /locus_tag="ACGI57_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9718079.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            1995..2684
                     /locus_tag="ACGI57_003024"
     CDS             1995..2684
                     /locus_tag="ACGI57_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFG9718080.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2687..3622
                     /locus_tag="ACGI57_003025"
     CDS             2687..3622
                     /locus_tag="ACGI57_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9718081.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3619..4512
                     /locus_tag="ACGI57_003026"
     CDS             3619..4512
                     /locus_tag="ACGI57_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFG9718082.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4590..5336
                     /locus_tag="ACGI57_003027"
     CDS             4590..5336
                     /locus_tag="ACGI57_003027"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFG9718083.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5337..6170
                     /gene="rsmI"
                     /locus_tag="ACGI57_003028"
     CDS             5337..6170
                     /gene="rsmI"
                     /locus_tag="ACGI57_003028"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFG9718084.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEIRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6193..6897
                     /locus_tag="ACGI57_003029"
     CDS             6193..6897
                     /locus_tag="ACGI57_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFG9718085.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
BASE COUNT         2923 a          681 c         1271 g         2122 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttctttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      481 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      541 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
      601 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
      661 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
      721 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
      781 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
      841 tgagataagt gaagagacaa atgtcttaaa agggatatct ataagtaatg tgattgatat
      901 aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat attatggaat
      961 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
     1021 agatgaagca catggtgcgc atttagggct tagcgagagg cttcctaaaa cagctcttga
     1081 gcaaggcgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
     1141 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
     1201 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
     1261 tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata ttataacttt
     1321 caaaaataaa gttgattcaa atattaatat atacgacaca aatgataaaa caaaaatatt
     1381 tatatcatct aaaaatatgg gtttaacagg ttatgaacta gaaaatattt taagaaacaa
     1441 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1501 aaatactgtt gaggatttct ctagtttaga aacagcatta aacaatattg taaaggaaga
     1561 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     1621 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagcatagg
     1681 taaagtttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     1741 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     1801 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa ataatagatt tagaagacat
     1861 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     1921 aatagactgc ttatatagag ctatctaata aaagtactaa aaaagtaaaa agtgtttgta
     1981 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     2041 gaaaggctac tcagacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     2101 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttggtgaaa atgtacctaa
     2161 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     2221 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     2281 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     2341 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     2401 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2461 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2521 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     2581 aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     2641 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     2701 tattataggg caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     2761 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     2821 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     2881 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     2941 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     3001 ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg ctataataat
     3061 cttagtcaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     3121 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     3181 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     3241 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     3301 aatgttagat aaaaacatag tggagatttt aaatatacca aataatatgg aaaaatatag
     3361 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3421 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3481 tatgagtgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3541 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     3601 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     3661 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     3721 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     3781 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     3841 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     3901 gaacatgagt taacaatgtt tttaatagat tgtgagtata catttgatag gaataagttg
     3961 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     4021 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     4081 ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt
     4141 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     4201 tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     4261 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     4321 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     4381 aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4441 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4501 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4561 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     4621 atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag
     4681 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     4741 gtactggaac tggaattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     4801 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     4861 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     4921 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     4981 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     5041 taattagagc tgcatccaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     5101 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ccaaaggtta
     5161 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     5221 aagatggaag gccagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     5281 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     5341 gtgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     5401 ctttaagaac attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5461 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5521 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     5581 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     5641 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     5701 gctctggtat ggacacacat agatttgttt ttgagggctt tttagataga gataaaaaag
     5761 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     5821 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     5881 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     5941 ctaccataaa aatatttgag gaaaaagaaa taagaggaga atttgtatta atagtagagg
     6001 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     6061 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     6121 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa aacctgaatc
     6181 tgaggtgatt ttatggaaat agggaaaatt ataatagaga gtttaaagag ggaagataca
     6241 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     6301 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     6361 ataaacgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6421 ctaagaaaac atgtgtcaaa ctatttaaac acatatattg aagaaggaat aaataagtta
     6481 catgtattaa agctgggaac atttttacat gatataggta agcctgacag tatgacctta
     6541 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     6601 aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag taaatatgta
     6661 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     6721 attaatgtat ttaacttagt agatgaggac actataggtg taataatgct tgggtatgca
     6781 gatatagtgt ctacagtaaa attattgggc aagagtggag aagtcagtgt gttaaagaca
     6841 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     6901 tagttaatat aattgagttt tagatgatta attgaagttt gaaatatacg gatttcaaaa
     6961 acataaaaga agttgtcttt gttgtaaaat tatgaaa
//
LOCUS       DAYAOX010000194         6986 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.195, whole genome shotgun sequence.
ACCESSION   DAYAOX010000194 DAYAOX010000000
VERSION     DAYAOX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.195"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(121..1215)
                     /locus_tag="ACGI57_003030"
     CDS             complement(121..1215)
                     /locus_tag="ACGI57_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718086.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            complement(1638..2579)
                     /locus_tag="ACGI57_003031"
     CDS             complement(1638..2579)
                     /locus_tag="ACGI57_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HFG9718087.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
     gene            complement(2572..3399)
                     /locus_tag="ACGI57_003032"
     CDS             complement(2572..3399)
                     /locus_tag="ACGI57_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFG9718088.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            complement(3570..3953)
                     /locus_tag="ACGI57_003033"
     CDS             complement(3570..3953)
                     /locus_tag="ACGI57_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFG9718089.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            complement(3922..4209)
                     /locus_tag="ACGI57_003034"
     CDS             complement(3922..4209)
                     /locus_tag="ACGI57_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HFG9718090.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            complement(4242..5399)
                     /gene="alr"
                     /locus_tag="ACGI57_003035"
     CDS             complement(4242..5399)
                     /gene="alr"
                     /locus_tag="ACGI57_003035"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFG9718091.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEKSVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            complement(5414..6004)
                     /gene="gerS"
                     /locus_tag="ACGI57_003036"
     CDS             complement(5414..6004)
                     /gene="gerS"
                     /locus_tag="ACGI57_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="germination lipoprotein GerS"
                     /protein_id="HFG9718092.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQDDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            complement(6025..6486)
                     /locus_tag="ACGI57_003037"
     CDS             complement(6025..6486)
                     /locus_tag="ACGI57_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFG9718093.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            complement(6501..6881)
                     /gene="acpS"
                     /locus_tag="ACGI57_003038"
     CDS             complement(6501..6881)
                     /gene="acpS"
                     /locus_tag="ACGI57_003038"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFG9718094.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
BASE COUNT         2165 a         1355 c          749 g         2717 t
ORIGIN      
        1 tgtaactaaa aaacatgttt tttctatttc ataataaacg agctaaaaaa taaaaagagc
       61 tatatgaaaa cattttcatt gttttcatat agctcttgag tttacaatat taaaaaatac
      121 ttatttgtca ctgattcctt cttgatatcc agatgttttt gtaatctgtc tataaagaac
      181 acaaaccagt attatgacgc ccatatatcc agtatatgga tataaaatac caaccagctt
      241 atcaaatggt aggaagccac ctataagacc taatatagct gctattaaag ttattaattt
      301 aaacttagga tgtttatcat ctacaaatct attagttact gaccataata aaggtacagc
      361 tgttgtatat atacctaata taagaattat tgagaataaa actccaatta ctggtgaaat
      421 cttatctgct aagaataatg atggtattgc ttttgaatat aagtttccta tatcagaaag
      481 aatagctaag tgcatcatta tagctgcagc cataagtgct actccaccta atataccacc
      541 acaaatagct tcttttttgt tagcagcact tgctcctaat ccagttaaga atgccataac
      601 aacaattgtg ttataagtag tatatattac accagataga taggcatttg gaactggttt
      661 agctatcttt acagtgctaa gaacttgtgc agcttctcct atattaccta tgtttttagc
      721 taaacttata ccaccaacta aaagtgtaaa gattattatt ataggcccta ttccacctag
      781 tatctttgat aatttatcaa gacctagtga aacagttaat aatgctacta ctgccatacc
      841 tactctacct acaaatggat ttaatccata gtattcagtt aaagttgctc ctgctcctga
      901 aatcattact acgaaaacac caaataagaa taaaggtcca aaccattcaa agaatattcc
      961 taaatactta ccacaataaa cacgataaat tttgataggt tctttaagtt gaagctcttt
     1021 accttttgac ataactgaag cacccatcca tgagaataat accattgaaa ttatcccacc
     1081 aataatacct gcaaaaccat aaaaagagaa gaattgcatt atttcctgtc ctgttgcaaa
     1141 tccagaaccg attactgttg ctacatacgc acctgcgaaa ctgatgactg ttttaacatt
     1201 tactttttct gacatatctc agttacctcc ccatgtgaaa ttttttcttc tgattaaaca
     1261 attcgataaa ctacctacaa tttcctttaa cataaaatac atgataactg ttttatagct
     1321 ttttagtata ataacttaaa acttataatc tgattttata ccctactcct cctctcataa
     1381 tttttttaga ttcttttatc taagtagtca ttatatataa aattagtttc tctataaatt
     1441 atatattagt catatttttc tgaatagtca atatatattt gaatattttc tctatttttg
     1501 taaaaatttg aatattttat tacatttttg atatattgat atcatataaa aaaaagagag
     1561 atataaatta atataacact ttatacctct cttttaaata tattatatta ttaacaaaca
     1621 tgaataattt atatctctta cattcttttt gcaataataa cttctttaac agtctttatt
     1681 atgtcatcac aagttaattc atattttttt aacaattcat ttggagtacc agattctcca
     1741 aatgtatctt taattccaac tttctttaca atggttggac aagactctcc tactacctca
     1801 cttacagcag aacctaatcc cccaattatg ctatgttctt ctacagttac aatagcattc
     1861 gtttcttttg ctgattctaa tattaaatct ttgtctatag gttttatcga ggacatgttt
     1921 ataactctag cacttatacc ttcttcttgc aacttttctt gtgctaatag agcttcattt
     1981 accataattc cacaagctat aattgataca tcctttcctt ctcttaagac tgtacctttt
     2041 cctatttcaa atttataatc ttcatcaaat aaaactggta ctgaacttct ccctaatcta
     2101 acataaacag gtccattata cttagctatt tcaaaaataa ttttttctgt ttcaactcca
     2161 tcagctggaa caagaactgt catatttggt atagctctca ttatagcaat atcttccaca
     2221 ctttcatgag atgctccatc ctctccaaca gtaagacctg catgagtagc acatattttt
     2281 acatttagct ttggataaca aactgaattt cttataatct caaaagccct tcccgtagca
     2341 aacatagcaa aagtacttgc aaaaggtatt ttaccagcag ttgataatcc acaagcagcg
     2401 cctattaaat tttgttcagc tatacccata ttaaaaaatc tatctggaaa tgacttgtaa
     2461 aaatcatgtg tttttgtaga ctttgataaa tctgcatcta atactacaac attgtcattt
     2521 atttgaccta attttactaa tgctttacca tatgcttctc tcgtcgccat tttactcatt
     2581 gtcagcacct cctaattcaa gcaaagcttt ttctagctcc tcatcacttg gagctgtccc
     2641 atgccatcct gcttgatttt ccataaaaga tattcctttg ccttttatag tttttgcaac
     2701 tatcattgta ggttttttaa ctgttgattt agctatatca agagccgcaa atatttggtc
     2761 aaaatcatgt ccatctatct caattacatt ccaaccaaaa gctttaaatt tatcaactat
     2821 tgaacctaaa ctcattacgc tttctatatc tccatctatt tgaagtccat tattatctaa
     2881 aaaagcaatt aaattatcaa gtttataatg tgcagcactc atagcagctt cccaaacaat
     2941 tccttcttga acctcaccat caccaagcaa agtatacaca ttccacggag cattatccaa
     3001 ctttgatgcc atagccatac cacatgcaac tgaaaaacct tgacctaaag aaccagtaga
     3061 tatttccaca cctggcgtgc ctttcatgtc tggatgacct tgtaacatcc ttcctatctt
     3121 ccttaatcca ttcagttctt ccttatcaaa atagcctttt tcagccaatg tagcatataa
     3181 tgctggtgca gcatgtcctt ttgataatac aaatctatct ctatcctcca ttttaggatt
     3241 tgaagaatcc acattcattt catcaaagta taacgcagta agtatttcaa ctactgataa
     3301 tgaccctcct ggatgtccag atttggctct atgtatcatt gaaactatat ctcttcttat
     3361 gatacgcgca atctcattta atcctttatg gtctctcatg caattacctc ccttgagttc
     3421 gtaattttat atcaattcca tattattata tcacttttat gctatttttt tgcgttattt
     3481 ttaattttat taacatagta cattaaaatt aaaccttaag agatatttaa aagaaaaaag
     3541 ggattgtcta ataaaagaca aatccctctt taaaaagtaa acaatcctaa acttatttga
     3601 agtccatcat ctacttttat catcatattt tcatctagac aaccaatttt ttccctcagt
     3661 ctctttttat cgatagttct aatttgctct aaaagaatta ctgagtcttt attaagccca
     3721 tattcattgg aacttatctc tacatgagta ggtaatttgg ctttattaat ctgagatgtt
     3781 atagctgata ctataactgt aggactatat ttattaccta catcattttg tattatcaga
     3841 acaggtctaa cgcctccctg ctctgaacca acaactggac ttaaatcagc gtaatataaa
     3901 tcaccacgct ttatttcaca attagtactc accttgggca atcttccctt catttttatc
     3961 atcagaagat acttcttcaa ctgtcatacc taattcagct agtgacagat taatttgacc
     4021 catttctcta taacccgttt tcatagattc tttaatatga attttctttt tctgagttat
     4081 atagaactga aaagcagctt ttgcaaaatc ttctctgtta ctgttctcca tctgaactat
     4141 actatcaact tcagaaatca gagagtcagg taaagtaact tctatttttt ctttactctt
     4201 atcgtacaca aaatcgcctc ccagattata atatcatggt tttattttag caaataactg
     4261 tttatttgta caagctcatt attttccata taaacacggt caactcttct tgatatcata
     4321 cataacactt catagtttat agttcctaag tctttagcta tacgctcagc tgtaacttcg
     4381 ccttctccaa atagtataac ctcatctcca acttttatgt ctatatcttt gtcaattcta
     4441 accattattt gatccataca tattctacca actacatcaa acacttctcc cttaataaga
     4501 acctttggat ttttttggat tctagtaaat ccatctgcgt atcctattgg aactgtagct
     4561 attgtttctt tacctgtagt tgtgtatttt agtccataac ttattcctac acctggttca
     4621 acttgtttta tatgtccgat ttttgatttt aatttcatgg ctggtcttaa ttctaatcta
     4681 tctttaaata catcatcaga tggataatga ccatataata ttattcctgc tcttaccata
     4741 tttaatctta aatcagggca atccataata gctgcactgt ttgatacatg ttttatagct
     4801 atttttacac cagcctcatc taatttatca gacataaatt tataattatt agcttgttta
     4861 taagtgtact ctttacttac ttcatcagct gtagcaaaat gagtaaacat accttctaaa
     4921 tctatatatt ctaatttatt taattctatt atttcctgta ctgacttctc attaggttgg
     4981 aaacctattc tagtcatccc tgagtctatt ttaacatgaa cacaggcttt ttctcctaaa
     5041 gattttgcaa tttcatttat cttttgtgct gtttctaaag aataaactgt catagttatt
     5101 ttatttttta tagaatcttc aaaagcttcg tctggagtat atcccaagtt caaaatagga
     5161 agtgttatgc cattttgtct aagttcaatt ccttcagcag ttcttgctac tgctaagtaa
     5221 tctacttttt ctttttctag caattttgca acttctactg caccatgtcc atatgcatca
     5281 gcttttatta ctccacaaat cttaatatct tcttctaata aatttttaat attatttaag
     5341 ttaaatctta agttatctag atttatctct gcccatgtag gcactgttat tttttgcatg
     5401 tctttagtcc cccttagttt ctgtattcaa aatctttgta ttttactgta aatcttggca
     5461 ctccctcttt atctaacact tccatttttt caggattttt tgtgtcagca tttacatata
     5521 atacttgctt gttaaaatat ttattatccc caggaataaa agtctctaaa accaaatgtc
     5581 ctttggataa ctttactttc atctcctcat tttgaagata gttttttata aagtctccta
     5641 caaaaagata ttgattattt tttcctgtat taggtaactc cactacatca ctaatctttg
     5701 gatttttaac taatatttta tcatcttgat attctatact tttccctttt aaatgttttg
     5761 gggaaattac ttctaattta tagttatcgg tttttttata agtatgaatt aaaacataat
     5821 tgtgtgggct tttatttcca actacttcta cttcagcttt acaactgtaa taattcatat
     5881 cagaaatctg cttttgaaaa tctttgtata cttcttcttt tgtggactgt cgtttttggc
     5941 atcctattac aattataacc agtgccaaga acataataca tactatggtc cacttttttc
     6001 tcacatcaca cctcctactt cagtttattc attgtataaa gcttttatta tatcatatct
     6061 acaaattata cctttaagtt tgttgtcatc atctactaca ggaactctgt ttattgattt
     6121 tttaatcatt acattagcta catcatcaaa cttatcatct tcatgaacct ttattatatc
     6181 tttagacata agttctccaa ccttataagc agctacttgt tttatatctt tttcaacttt
     6241 ttttaagtca tctaagaaaa tcaatccttg taatagattt atatagagag gtgcttctat
     6301 atatttttct tttttaagaa tatctgtttc agaaattatt ccaacaactc tattctctga
     6361 atctacaact ggcagacctc ctattttttc tgcaattagc atattagcta catctgctat
     6421 actatcatct tgttttgcta ctattacatc tgttgtcatt atttcttttg ctgttttatc
     6481 catcataatg taatcactcc ttagtcctta attatggtta tcgcatttgc tatagcataa
     6541 tcttttgaat gtgatattga aacttttatt tctaaaacat tataatctat gcacattttt
     6601 gctagattat tataagtttt tactattggt tttcccattt catttctgag aacttctata
     6661 tctttaaaat taaacaatct aattcctgta cctatagatt tacttatagc ttccttggct
     6721 gcgaaattac ctgctatact ttctgcttta aaattcttag aattaaaata ctctatttct
     6781 ctatctgtaa atatcttctc caaaaaccta ttatttttgt ctacagcctt tcttattcta
     6841 tcaatttcaa ttatatctac gccaatatca aatatattca ttgttcctcc agaaaattta
     6901 taatacttct ttcaataatt aataatgaat ttataaattt actatcgata atacacatta
     6961 ttatttagtt tatgttactt atctta
//
LOCUS       DAYAOX010000195         6984 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.196, whole genome shotgun sequence.
ACCESSION   DAYAOX010000195 DAYAOX010000000
VERSION     DAYAOX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.196"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            242..1069
                     /locus_tag="ACGI57_003039"
     CDS             242..1069
                     /locus_tag="ACGI57_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HFG9718095.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2094..2558
                     /locus_tag="ACGI57_003040"
     CDS             2094..2558
                     /locus_tag="ACGI57_003040"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9718096.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(2825..3469)
                     /locus_tag="ACGI57_003041"
     CDS             complement(2825..3469)
                     /locus_tag="ACGI57_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718097.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMVPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3399..4688)
                     /locus_tag="ACGI57_003042"
     CDS             complement(3399..4688)
                     /locus_tag="ACGI57_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718098.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4685..5557)
                     /locus_tag="ACGI57_003043"
     CDS             complement(4685..5557)
                     /locus_tag="ACGI57_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718099.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            complement(6087..6578)
                     /locus_tag="ACGI57_003044"
     CDS             complement(6087..6578)
                     /locus_tag="ACGI57_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFG9718100.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT         2557 a          952 c          823 g         2652 t
ORIGIN      
        1 aaataatttt aacatgccca taaaatataa tacccacaaa tgataaataa tactcaaaat
       61 gtctattgaa ataactatac atccttaaat aacttaactt aaatctaaac atttcaaaaa
      121 cttcttaaaa atatgtaaaa atttgatgat aaagtagcga ttttttcaaa aaaaatgtaa
      181 tatagagtta tatcttataa tattttagag ataaatttta tcatagtggg gagaaatttg
      241 tatggattta agcaaaaaag taaaaatttt agaaaatgca tcagatggca aaagcgaatt
      301 tgagatatta gaatttgaaa aacttgaggg agcttctagc ccattaatgg caatgggatt
      361 atattttgct agagaagctg gtttgaaagc tagacaaatt aagataaaat taaataattc
      421 atcaataaaa acagaagctg gtgcattata ctattataaa ggaaatatat cttctgaaac
      481 taagattggt ggtgttggag gtctttttaa aaaaggaata tcaggagcac ttactaatga
      541 atcaatgatg aaacctgtat acaaaggtac tggagaagtt tatttggaac cttcttttag
      601 acattattta tttatgagtc ttgataatga ttctataatt gttgataaag gattattcta
      661 ttgttgctct gatacaatag aagtaaaagc atttacacaa aagaacttct catctgcttt
      721 acttggtggt gaaggcttct tccaaataga attaagtggt actggtgtaa tcgttctgga
      781 atgtgttgta cctcaaagtg aaattgtaga gtatgaattg aaaaatggtg aagaattaaa
      841 ggtagatggt aactttgcaa tagcaagaac atctggtgtt aatttttctg ttacaaaatc
      901 agataaatct ttaattggtt ctgctttaaa tggtgaaggt ttcttaaata catttactgg
      961 taatggtaca gtttggctag ctccaactca agtaatgtat gaaaaactaa gttttggagc
     1021 aatgcctact aatgataata tgaataataa agtttctaag caaaaatagt tgttttataa
     1081 atatttatta aatcattatt gattttataa agtcactata aattttatta gattattatt
     1141 ataaaatcag agtgtggaaa tgtttatgtg tattctttaa ataaaaattt ccacattctg
     1201 taaggttatt atacttgata tataaaattc aatttaggaa catagtctaa catttataaa
     1261 catattaagg taatgatttc tcttggtaat tattctcata tcttattctt tgattatatt
     1321 tatcttattc ctagccctta tgtcatatta tatttcttat cttattactt ataattagca
     1381 ttaacttatc taaaatcttg tattttttta atagtaaagt ttacatctat attaaaaagt
     1441 ttacatctat attaatttca taaaacaatc aaggtagtca agagattcaa ctttacttga
     1501 ttttaaaaat ttaagtgaat ttgttcttat acacatcttt ttaaaattat atctgtagaa
     1561 aatatcattt agagctataa ttaaattaac ttattaaatt ttagaaaatt tattaaaaaa
     1621 tataagttaa attaatattt ttaaagattc ttttttgtaa ctactagaat agtaatttag
     1681 tagttattgt atctctaaag tttaagcatg gatagatata catttgataa tttgcctata
     1741 atttacctac tacttggatt attggagcaa atacaatata ttaataataa gttcctaaaa
     1801 ccaataaatt aactaataca cataaagata ttgatgttga ttatacaact ataagaaacg
     1861 gatttaacag aatttaatca agctaagaaa ttatatatat atcgtgatgt ttttgaatta
     1921 gaaggtaatt caaaattagt ttatagtctt ctgtcaatcc aataaaaagt acagggtaca
     1981 agaattgtta gtaaaatttt taaagatttt atggataaat ataaatagtt ttacttattt
     2041 tctaaaactt taaaatacca ttaattacct taaatatttt aataggagga catatgaatt
     2101 taaataaagt taaatttact gaagaacatg ctattgaagt tactaattgg aaatatgaag
     2161 gtaaatattc aatatacaat ctacctccat gggaggaaat taaaaagaag aatttttcac
     2221 tagcaaaaga agataaaaga aaaaatttta taagttttat aaatgacaat aaagaattga
     2281 taggttttat aaacttatta gatgaaggaa gctctgtatt ctttggtatt ggtatgaaac
     2341 cgaattattg tggaatggga attggtaaag aaataattgg cctaggatta caagaatgta
     2401 gaaataaata tagtactaaa ccaattgtat taaatgtaag aacctggaac atgagagctg
     2461 taaaatgtta tgagtctcaa ggatttaaaa tagttgagac taaggtacaa aaaacgcatt
     2521 taggagatgg agaatttttt gttatgaggt atcaatagtt tataacaata gtcttattgt
     2581 taatacaaca attaaaatga aaaaatacat atgtgtaggt catagcaata agtgtctttg
     2641 aaatataaat ttgagagctt ttgataaaat tttgtataca tatatatttt atactacgga
     2701 tatataaatc tatctacata tataaagaga ttgtaagtag gtttgtgtat ataaaaatag
     2761 atttatattt cataaatgta ctttttattt gtattacctt tattttatct aagctctcat
     2821 tttattatct aatcttcctt tttactggtt ttattaagag agatgcaatt atcaacacaa
     2881 cacttccaag tatcagccaa tatttctcat caaaactacc ttgactcata gacattataa
     2941 aaaattttcc aacatacttg cctttagtca tcatatttaa aattaaatat cctatagata
     3001 tcatataacc tatgattgta ttattaaata aatttgagaa aaacattcct aaacttccta
     3061 taaacatagc tgatgcgaaa cttccaataa tatactcaac tactggaaaa ttacatccaa
     3121 gatatattaa aacatttaca aaacctatga ttaataccac tatacctata attgacatca
     3181 gtatcctaat taattgtaca ataacttgag ataatgcttt tgaagatact atctcatcaa
     3241 tttcttttga ttgctctagc gaaaatacag gtaccaacat tactatccct attattgcaa
     3301 cataattttc taaacatata gctgttgaaa ttctgtctaa attagctact ccttgtatta
     3361 gaggaaccat acatagcaat ataattgaac atattaaatt agttaaagtg cttaatttga
     3421 agttttcttt tattagaact aaacagtttt gaaaactttg cccaaacatc aagattcccc
     3481 cttctttttt gctcatataa gaatatagct aacaaaacaa gcaccaatgc tcccaagctt
     3541 atcacaattc tattaatcat aatttgattg aagttttgta taataatatc cccatttaat
     3601 acactattat gtcttatcat tagccccatt gttgaataat cacctttgag tcctgtagaa
     3661 tttatactgt aaagccacca aattaattgc acaataattg ctactggtgt atctgttaat
     3721 atagtaatac tcattcctac agctgtaact atcataagag ttggtagtat ccaccataat
     3781 gtatatttta taaatgctaa gttatcaata gatatacctt tatctttagc aaagaaaatc
     3841 aaaggaagta aatctttcat acctaaaatt aatacaggca atatcatcat aaagacaagt
     3901 gcaaaatatc ttgataaaac tattttgcat gaagatactt gttttgaata aataatctct
     3961 ctcattcttg aacgcttatc tttaagtgac ataaaaactg ccacaaatac tggaaatata
     4021 cttaaaacta tacctatata atcgcaaaaa attcttgcat acccattact aactttatct
     4081 ttttgtattg tatagttgta attcttcata gcttcatcat aggtaactgg aactactcca
     4141 aatctttgta acttgtcaaa agcatatgca gaccctcctc ctaaaatgtc gtctactctt
     4201 tgcattattt cttcaaatct ttcaagagat actttagaat aatctatttt tatactttca
     4261 tcagttttcc aaagtgaatt tatttcgcta atacttttac cagttatttc ttctatatat
     4321 ttttctatgg tcttcgtttt attttggtct aattttatat acttaactac ccctgttgga
     4381 tacgttgtat atgtgttttt ttgatattct ctaattaggt ttatagttgc atttggaatg
     4441 accttttctg ttttcataga ttctttttga ccatatggat attgattgtg tacagaataa
     4501 tattcctcaa tattctctac tttttgtgga gcaactaatg gatttccatc attaggaata
     4561 tttttactta catcatttga aactgaactt ccaagttgag aaatatagaa aaaaactaaa
     4621 actacaataa aaatataata taccatactc tttaatatat ttttacattc ttttataaat
     4681 agattcatat attatctcct cccattataa gcatatagcc atcttcaata gttggattac
     4741 aatttactgc tgtctcaaaa ggtttatcat ctgatataat cctaagtata gcctttccac
     4801 catgagtcaa cattcctata actataaatc tactttttat attttcaatg tcttttttat
     4861 ctacttcaat actatatact ttcccttcag ctagttttgc taatgcttct gttgagccat
     4921 tgtatatgac ttttccacta ttcaatatag ctacgttttc acatgtagct tctatatctc
     4981 caacaatatg tgtagataaa ataacgattc tatcatttgc aatttcgcat agtaaatttc
     5041 taaatcttat acgttcttct gggtcaagac ctgctgtcgg ctcatctact attaaaactc
     5101 tgggattatg aattaatgct tgtgctatcc caagccttct attcatacct ccagataaag
     5161 attttacttt cacattttga taattagtaa ggtttacttt gcttaataac tttgctataa
     5221 tttttcttct ttttcttagt ggtatatccg ataaaactgc taaataatcc attgctttat
     5281 atactgtcat gttaggatac attgaaaaat cttgtggcaa ataaccaacc atatttctaa
     5341 tttccttagt attctcaata ggaactccac acatatgtat tgaaccttct gtttttgata
     5401 ataaagtggc taaaattctc ataaatgtgg tttttcctgc tccatttctt cctaaaagac
     5461 caaacatacc actgtcgatt tcaagattta tattatcaag agcttgtttt ttcccaaatc
     5521 ttttactaag attggatact gctattttca tttccatata taaatacacc cccagaaaca
     5581 attttataga attttatttt tataaatttt tactgtattt tcataataaa ttctaaaact
     5641 taagatatta tttataaatt tctaaatttt ttctaaagat tttaattttt ctgtttttaa
     5701 ttaaattaaa gtatctttgt ttctgaaatt ttttaagttt atgaagttat aatttaatat
     5761 atatttattt tatttgtgag tctattttta tatataatga aaaatgtgat aagtatccaa
     5821 aagaattact ggatataata ggatagggta tttagccttt taagataaat aaaaatatct
     5881 acaactatat attgcaaaaa tttaaaacta atttatatat gctaatataa atatttataa
     5941 agacataaga caaatttacc tacagattta aatttaactt atatatttta aaaggctaat
     6001 ataatatgta ttttcttcta tacaatcaaa aggcttcata tctaagatat gtgatgacac
     6061 taatattcac aattttttag taattatcac attataaaaa atattacagc tagtccatca
     6121 tcagatgctt cttgtataaa ctttttaagc tcaattagat ttgcccatac atattcaaaa
     6181 aaatcatctt cactctcatc attcataaca ggataaatat tattttctaa catctcatta
     6241 atattaaatc tttttctaaa tgcttgttct tccaaactgt tcattgcttt tgatagtttt
     6301 tttacatcat ttgctgtaat caacatgaca ggaaattctc catcaatttc cataagtatg
     6361 tttccactta gtactaattt gctaaatata ttatcatcat ctccaccaaa aggacaacct
     6421 gtcaaagtaa agtgaatggc atgccatgct ttatcaatat tgatagtact attttcgttg
     6481 gcatcttcaa aaacaaaatc ttgtaagttt tcttctgctt gctgtaattt aaaaacattc
     6541 tcttcactta ttttagtata acatcctatc atccccataa ttacattcct cctaagtagc
     6601 gtttttttaa ttgtaactta taattaaact tagttacaat aaaatatatt gagaaaagta
     6661 ttatttcatc ttaaatttaa atacctatat tatcaactaa aattctgtgt ttatagcgta
     6721 agtattatta ttttttccta aaaattaaat gaatcagaag ttctataggc aatattgact
     6781 ataaataaga actattactt ttaaaaaaaa atttaagaaa ttatactgta gaatcatgct
     6841 ctacttatta gctttaaata aaaaataaat catgttctca aattaaataa taaataacaa
     6901 taaatttaaa ttttaaacta taagagtttc tgaagtgtta ataagcatac caataattat
     6961 atgtgtaaga atttgattta atag
//
LOCUS       DAYAOX010000196         6783 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.197, whole genome shotgun sequence.
ACCESSION   DAYAOX010000196 DAYAOX010000000
VERSION     DAYAOX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.197"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            119..1108
                     /gene="add"
                     /locus_tag="ACGI57_003045"
     CDS             119..1108
                     /gene="add"
                     /locus_tag="ACGI57_003045"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFG9718101.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            1180..1920
                     /locus_tag="ACGI57_003046"
     CDS             1180..1920
                     /locus_tag="ACGI57_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HFG9718102.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPSLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            2120..3271
                     /locus_tag="ACGI57_003047"
     CDS             2120..3271
                     /locus_tag="ACGI57_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFG9718103.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            3296..4051
                     /locus_tag="ACGI57_003048"
     CDS             3296..4051
                     /locus_tag="ACGI57_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HFG9718104.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            4243..6630
                     /locus_tag="ACGI57_003049"
     CDS             4243..6630
                     /locus_tag="ACGI57_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HFG9718105.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         2739 a          696 c         1263 g         2085 t
ORIGIN      
        1 aaatatttta atgagatatg acttttttag aattaaaaac cgtagtatat tgatataata
       61 gaaataagac ttaaaaatct gttgatagta ttatttataa gtacagtagg aggataaaat
      121 gtttgaaaat ttacctaaga tagatttaca ttgccactta gatggaagtg ttagagtaga
      181 aaccatgctt gatatagcta ttaaagagaa aatagattta ccttcaaata atatggatga
      241 aataaaaaaa ttagcaaagg tttcttttaa ttgtacttca ctagatgaat atttagaaaa
      301 atttgattta cccttaaaag taatgcaatc taaggaaaat ttaaaaagaa ttacttttga
      361 acttttggaa gatgcaagta gagaaaatgt aaaatatatt gaaattagat ttgcaccatt
      421 acttcacacc caaaaaggta tgagtgttaa aaatataatt gagggtataa ttgaaggaat
      481 tagagaagct gaaagtattt atgatataaa aggcaattta atattaggtt gtatgagaac
      541 aatgacctca aaagaggcat tattagtaat tgaagaaggg aaatcatttg taaataaagg
      601 tgttgtagca gtagacttgt gtggtccaga gaaagaggga ttttgtaagg aatataaaga
      661 tgtttttaaa ttagcaagag aatatggata taaagttaca atacatgcag gagaagctgc
      721 aagtggagaa aatgtattgg atgctataaa tattttaaag gcggatagga ttggtcatgg
      781 ggtaaaaatt aaagaccata aaaaggcgta taatttagtt aaagataaaa aaatattgct
      841 tgaattatgt ccaactagta atgtacaaac taagacagta gattcttatg aagcacatcc
      901 attttatact ttttataaag ataatttaca tgtaagtata aatactgata atagaactgt
      961 atcagatata aatttaagtt cagaactaaa tgttatattt gatactttta aactaggatt
     1021 agaagactat aaaataattt atagaaatgc agtagaagct agttttgcag ataaagaaac
     1081 taaagagtat ttgaatagtt taatttaata gataaaaata tttaaacttt aaattaagct
     1141 attaaattga taaaaataca ttgtattagg agaaaaaatg tgaaagatta tgaagatgtt
     1201 gtttatatta aactagtcaa aaaagaactt gaagaatgga agaaaaatat attaaagaaa
     1261 ccttctatat ttgacaaagc atcaaagggt atacagaata aatttaatgg agtattgcca
     1321 gataaatatc atgaaataat tacttcttct ataaaagtaa tgacaaaaac cgtactgttt
     1381 ggatataaat atgctacaaa gccatcactt agaaatatgt cattagaaga aagagaaaaa
     1441 ttagttttag aaaaagctaa aacttacaag actacagcaa tgattgaagg ggcaggaaca
     1501 ggggcaggag gaatcctctt aggtttatct gattttcctt tgcttttagg tattaaagtg
     1561 aaatttttat atgatgtagc ttcaatatat ggatatgatg ttaaagatta tagagaaagg
     1621 atttatatat taaatatatt acagttggct ttttctagtc aacagcatat aaacaaaact
     1681 tttaaattga tgaataactg ggatgaatat gtaaatagtc taccagaaga catcaatgat
     1741 tttgattgga gaacatttca acaagagtat agggattatt tagatttagc taagttttta
     1801 caactaatgc ctggaatagg agcatttgta ggattttatg tgaatggaaa attaattgat
     1861 aaattatgtg aaactgctgt gtctgcttat agattgagga ttcgagagct taagttataa
     1921 gagaaattat aacataaaaa attagttata caatatatct atatgtatct aattttgcat
     1981 tgataaaaac aattatggat aaaaggtaaa gcatgataga atctaatgta gaggtttgta
     2041 ttttaatgga aaaatttagt ttttattgct taagatacaa attgaattac gaagagaagt
     2101 aattgggagg aatattttca tgacaaattt gccaaaacaa tttgatagct tcaatgaagc
     2161 tagacaaaaa ggatttataa atgcaaagga acttaaggaa agtgggaaaa agatggttgg
     2221 agttttctgt acatttactc cagttgaaat tccaatggca gctggagcaa ctgtagtagg
     2281 tgtatgcggc gttagtgaag agccaatacc tgatgcagaa agggtgcttc caagaaatct
     2341 ttgtccttta attaaatcaa gctatggaca tgcaataaca gatacctgtc cttatttcta
     2401 tttttctgat ttgttgatag gagaaaccac ttgtgatggt aagaaaaaaa tgtatgaaga
     2461 acttgcaaaa gttaagccaa catatgtaat gcatttgcca aatacttcaa aaggagaatt
     2521 tgcctataaa ctttggaaag atgaaatgat aagattaaaa gaagaggttg agaaatcttt
     2581 aggtgtaact ataactgaag aagatataag aactgctata aaagataaaa atgaagaaag
     2641 agaactttta aaagagtttt atgcacttgg taaattacaa ccatctgcac ttacaggcct
     2701 tgagttacat aatgttttat atcaagcagg ttttaaattt gaccgtgcag aacttaaaca
     2761 ttctttaaga aaagttatag atgatatgaa agaaagatat gaaaaaggtg aatgtcctgt
     2821 tcaaaaagat aagccaagaa tattgataac aggttctcct attggtggaa taagcgaaaa
     2881 aatagttaaa actttagaag atgcaggggc atcagtcgtt gcatatgaac tttgtggagc
     2941 tattagatca aacgatttat tggttgatga agaaatagaa gatgtatatg atgctttaac
     3001 acaaaaatat ataaatatag gttgttcttg tatgatgaat aatgacaatc gtattgaatt
     3061 attagataga ataatagatg aatataatgt agatgctgta atagatgtag ttttacaagc
     3121 atgtcataca tttaatatag aaagttatag aataagagaa tttgttacaa aagaaaaaaa
     3181 taagccattt atgtctcttg aaactgatta ttcaaaatca gatactgaac aattaagaac
     3241 tagatttgaa gcgtttgtag agatgttgta ggaataaata aaagtaggag gaactatgtt
     3301 tagtatagga gttgactcag ggtcagtggc aacaaagggt gtgctatttg atggtgaaaa
     3361 aattattaag aaaataatta taccaactgg atggagccct aaaagtacat ctaaacaggt
     3421 ctatgagttg ctaagtagtg aaattgacaa aaaagatata aaaaaggtgg taggtacagg
     3481 ttatggaagg ggagtaatgg attttgctga taagaaagta acagaaataa cttgtcatac
     3541 tagaggtatt tactttttaa acaagaacat aaggactatt ttagatgtag gtggtcaaga
     3601 tagtaaagtt ataaatttag atagagatgg aaatgtattt aactttataa tgaatgataa
     3661 gtgtgctgca ggaactggtc gatttttaga gattacttct aaccttttag gaagcgatat
     3721 tgaaagtata gatacattag ctaaaggata tgaaccagtt aatatatcta gtatgtgtac
     3781 agtttttgct gagtctgaaa ttgtaagtct tctagctcaa aacatttcta ctggagaggt
     3841 agcagcaggg atacttaaat ctattgcaaa taaatccacc tcaatgttag caagaggtga
     3901 ggtaatagat gaagttgcat ttactggagg gcttgcaaaa agtaaagaac tagtaaaaat
     3961 gatagaagaa atactgggta aaaaaatttt tattgcagaa gatactcaaa taataggagc
     4021 tttaggagct gctgtaatag gatttagata aaaacatata aaaggaaaat gagaagctga
     4081 atttattata aataaattca gttttttatt ttattttatt tatttataaa ggaaaacttt
     4141 ggtataattg atttaaatga ttgtatattt gtaaatactt gatatttagt catatataac
     4201 attataattt taaaaattag ctcaaaataa gggggagaaa gcgtgaaaaa atttacttca
     4261 aaaaaagtaa caaggtttgt tgcgctgttt ttaatatcta ttttagtaat gtcaacaatt
     4321 cctgtatcag ctgacaatag caatacattg aatcagataa aaaaagcaga atacagtgag
     4381 acgtataaac aatatctaaa tgatgctaaa aatggacgta cagaaaaata taatgggatt
     4441 ataccaaatc catatcagtt ggaaggtact caaatccaaa gtaaaggaag aacagctcca
     4501 acatcttatg accctagaaa attaggtctt atgacatcaa taaaaaatca agaggattta
     4561 ggaatctgtt gggattttgc tgcaatggca accttagaaa gtttcttaaa gttaaataat
     4621 tatggagact atgatttatc agaagaacac ttaagatggt gggcttcaga tggtgaatat
     4681 ggatggtctg taaatgatat gaatggtgct ttaaattatg aagctatggg atatttaaca
     4741 tcttggtcag gtcctaaact agaaaaagat attccataca atggtagagt atctaaagca
     4801 caaggagcaa aaaaaccaac taatatgaat acagcaccta ctgtttttaa tgtgacagat
     4861 gctgtgtgtg taagtaatga cataaattct acaaaaaatg ctatattaca gtatggagca
     4921 gtaacatctg gttattatga agatattaaa tatcaaagtg atgaccaaaa ttcatattat
     4981 tgtccaacaa aaagttttaa tacaaatcat gctatttcaa ttgtaggttg ggatgataac
     5041 tattctaaag ataattttaa tgcaaatatt agaccatcaa aaaatggtgc ttggttaata
     5101 aaaaatagct ggggagatta taattctgag ggtggatatt tctggatatc ttatgaagac
     5161 aagacactta tgtctgatat agataatttt tcaatcaaag gtggtaaaaa accaaatgat
     5221 gatgagaaga tgtatcaaca tgactatgca agtattgtac ctttaatttc aaaaaagata
     5281 acagctgcaa atgtatttga tttcaataga ggcgatgaaa ctctaaaatc tgtaatgttc
     5341 ttaactgaaa gtataggagc taagtatgaa gtatattatg ctccagtagt aaatggtata
     5401 cctcaagaaa acaatatgaa aaaattaaaa gaaggtactg cccaatattc aggatacata
     5461 acagttccaa ttgattcttt tgatatacca gaaggaaagg gtgcaattgt agtcagtata
     5521 gaggggaaaa atggagagtc aactataggt tctgagtcaa atgtaccagg atatgataca
     5581 tttaaagcaa aagctaattt aggtgaaagt tatatcatag ataatactgg aaaattcttt
     5641 gatataaata gagatagtaa cttctatccg tgtaatttta ctataaaagc tgttacagaa
     5701 aagtcttctg gtgagagtat cccaaatgag tctttaattg gtagtgatag atatgaaact
     5761 gctatcaaag ttagtcaaaa tggtttcaat tcttcagaaa atgtagtttt agtaaatggc
     5821 agttcaatag tagatgcatt ggcagcaact ccttttacat ctgctataaa ttcaccaata
     5881 ttattgacac aaaaagaagc tttaaattca aaaactaaag ctgagataca gagattaggt
     5941 gctaagaaag tatacctaat aggtggagaa aattctataa gtaaagaaat tgaacaacaa
     6001 ttaaaaagct taaatatatc gatagagaga atatcaggaa gtgatagata caagacaagt
     6061 ttattattag ctcaaaagtt aaatggtata aaaaatgtat ctcaaatagc agttgtaaat
     6121 ggagtgaaag gattagctga tgcaattagt gtaggcgctg cagcagcaga aaataacata
     6181 ccaatcatac ttgcaaatga aaagagcgaa ttgcaaggag ctgatgaatt tctaaattca
     6241 ttaaatatag agaaatctta tattattgga ggaacagcat ccctatcaaa caatctagaa
     6301 agtaagttaa aaaatccaac tagattatct ggtagtagta gagacgaaac taattcaaag
     6361 ataatagata atttttataa aaaagatact ttaaaaaatg catttgtagt taaaaatgga
     6421 atcaagaatc aaaatgattt aatagatgga ttggcagttg gtcctttagg agcaaaaact
     6481 gggtcaccag taattttagt tggtgataaa ttagctgata gccaaaaaga agttcttaaa
     6541 aataagactt tagaaaaagt aactcaagtt ggtggaggag caaataaaaa tgcatttaaa
     6601 gagttagtaa aattaaaaag tgctaaatag agtacttaag tattaacata tattagtgtg
     6661 tattagtaac ttatcttaat aaaagctgaa aataattact tcaaattatt gataaataag
     6721 ataatcaatg attgtaaagt tttgttttca gctttaaatt tatgtagtat aagtttaaaa
     6781 aca
//
LOCUS       DAYAOX010000197         6769 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.198, whole genome shotgun sequence.
ACCESSION   DAYAOX010000197 DAYAOX010000000
VERSION     DAYAOX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.198"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            145..906
                     /locus_tag="ACGI57_003050"
     CDS             145..906
                     /locus_tag="ACGI57_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6873 family GME fold protein"
                     /protein_id="HFG9718106.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            936..1727
                     /locus_tag="ACGI57_003051"
     CDS             936..1727
                     /locus_tag="ACGI57_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HFG9718107.1"
                     /translation="MKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKKILNGTKAEQ
                     RALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSLNIFAKKEIF
                     MRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLIKKDQDEFIR
                     VLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVIRENLNYEDF
                     LISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            1907..2509
                     /locus_tag="ACGI57_003052"
     CDS             1907..2509
                     /locus_tag="ACGI57_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HFG9718108.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    3121..3359
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3423..5342
                     /gene="thrS"
                     /locus_tag="ACGI57_003053"
     CDS             3423..5342
                     /gene="thrS"
                     /locus_tag="ACGI57_003053"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFG9718109.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            6110..>6769
                     /locus_tag="ACGI57_003054"
     CDS             6110..>6769
                     /locus_tag="ACGI57_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HFG9718110.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MD"
BASE COUNT         2740 a          646 c         1197 g         2186 t
ORIGIN      
        1 accaactgta caaagctata ctaattaagc ttagaataac tatataaaat tataacaata
       61 agtgtgtggg cagaaaaatt ctgctacaca ctttatttat attcacataa aaaatttatc
      121 aaaaaaaaga aaaaggagta aaaaatggaa tttacaagag aaccatttat actagatgac
      181 gatttgtgtc tagctttggt ggacaaaaga attagttatc taatggaaaa tgaattaaaa
      241 ttaagaaata tagagataat aaaaacaata gaatgtaaag aattatatga aagcataaaa
      301 tatcacccgg atatatgtat ttgtaattta ggaaaaggag atataattgt tgcgccgaat
      361 gtatataatc aatacaaaga gttattaagt agatataaat ttaatataat taagggagaa
      421 gctaatttat taaatagata cccttatgat ataccttaca atattgcgat agttggtgac
      481 tatgctatac acaattttaa gtatactgac aaagcaatat tagattatat tgaaaataat
      541 aaacttaaaa agataaatat agagcagggc tattctaaat gctcaatctg tatagtagat
      601 agaacgtcta taataacatc agataaaggt atttggaaaa gtatgaagaa tacagaaata
      661 gagtgtttat tgatagaaaa aggtcacata aatttatttg agatgaatta tggatttata
      721 ggtggatgta cagggttgat ttctaaggat aaactagctt tttgtggaga tgttaaaaaa
      781 catcctgact atgaacgtat aaaaagtttt gtagaaagta aaaacaagga gatagttaca
      841 cttagctgtg aaaatttact agatataggt tctatagttc ctctcatgac aagaaaggag
      901 cgataacttt ggttgagaag gataatattt cttccatgaa atttttgagg aataataatg
      961 taaacgatag attaatatat acgaatgaca ttgtagaagt agtgatagat tctgcaaagc
     1021 aagaagtgtt aaataaaaaa atacttaatg gtactaaagc tgaacaaaga gctttaaata
     1081 ttgatgagat atcaacggaa ataactagta tgataataag tataatgaaa gagaaacttt
     1141 tgaaagaata cattctaaaa tgctatgggc gtacatatcc tgaagataaa aataatatat
     1201 acatatattc actgaacata tttgctaaaa aagagatttt tatgagagaa acagtattta
     1261 ctagggtgag aaattatata gaagaaaatg attttataaa tgtagaaggt tttattagat
     1321 ttagaatgag agaatttatg aaatatattt ctgctatagg tgatattgca gttgaggagt
     1381 atttaataaa gaaagaccaa gatgaattta taagagtttt aaaatacttt atagatactc
     1441 aagaagaaaa gattgatttg cttaaagttc atataatgga agataataca tttgttttat
     1501 acgataaaaa tggaaataag atagacagta tagatgatga agaaattata aatatggtaa
     1561 taagagaaaa tttaaattat gaggactttt taataagtac cttattaact ctatgtccca
     1621 aaaaaataga aatattggat ttgttaaata ataattgctc aaaggaaatt atagatactg
     1681 tagaagcaat ttttgaaaat aaagttagta taattatgga aaactagaat ctaagcatat
     1741 aaaagctatc catataaaat taaaaaaatt ctttttaaat cgacacaaaa gacttataat
     1801 tgtatataaa gctatagtat aactagagtt attaagtata aagaatatct acttgattaa
     1861 tgcaatatag ctggcaaata tacaattgat aaggagtaat tttataatgg ataatttaat
     1921 taggatactg atattagctg ttattggtgg atttataggt tatgttacga atgtagttgc
     1981 tataagactg atatttagac caatagagcc tattaaaata cctattttaa atatagaaat
     2041 agtaggtctt ataccaaaga gaagagcaga aatagcagca aatgtaggag aaataattca
     2101 agaagaattt ctttctatgg acgaaatact tgcaaatatc attactgacg aagataagga
     2161 agaagttgta agatacataa aagcaagggt aaaaattata atacatgaga aagtttcatt
     2221 tatcccgagt ggtataaaaa acatgataca agattatcta ggagagataa ttgagtctga
     2281 agttaagcaa agtatagatg aattgagtaa aaatataata aataaagcaa atgaacgtat
     2341 agatatacaa aaaatggtcg aagataagat taatgaacta gatttatatg aattggaaga
     2401 gataataata agaatagcta agaaagaatt aaaacatata gagtttttgg ggcttgtctt
     2461 agggttttta ataggtatag ctcaagggtt aattactatg tttatttaaa tatatgagaa
     2521 gtagcattta tttaagtact aattatattt acctatttga gtgaattact tattaataac
     2581 atttagatat taattgtatt tacctatttg agtaaattac ttattaatag catttagata
     2641 ttaattgtat ttacctattt gagtgaatta cttattaata gtatttagat attaattgta
     2701 tttacctatt tgagtgaatt acttattaat agcatttaga tattaattgt atttatttat
     2761 ttgattgtgt tacttattaa tagtatttaa gtatttactg tactacttat taaccatatt
     2821 aagtattgat tgtatttaag tatacaaaac ctaaaaagag tacaaaaatt agaagttagt
     2881 tttaataaaa attagtttta gtatttatct agtcatatct atcatgcatg tggatacgtt
     2941 ttaaaaattt taaatttatg aaaatattat aatataacta taaatcaaga gattaattta
     3001 atataaaaat ataagaatat tggtttacag ctgattgaga tgaaaaataa tgggtaatta
     3061 atatattaca ttaatgttga ttgatgtata actatctgtt ataatgaaaa tatattaaaa
     3121 atcatagtgt tgaagaggaa gagtagagta gaaaccattc tcagagaatt tgggtctatg
     3181 gctgagagcc catgtaatag gtctatttga aaactacctt ggagctgagc ttattagcag
     3241 tatttgtatt tatactatac gtgaaagtgc tcgggtgacg accttatagt catcaagagg
     3301 gttgtatata cttgtatacg ccaacttggg tggaaccgcg aattaatatc gtcccaaaca
     3361 tatttggggc gattttttat tacaaaaaat ctaaaaatta tttcaataag gaggaaaaaa
     3421 caatgattaa agttgcttta aaagatggct caataaaaga atttgaaaat gctatttctg
     3481 ttatggatgt agctaaatcc attagtgaag gattagctag aaatgtggta gcagcgtctg
     3541 tgaacggaga ggttgtaggt ctggaccaca ttatagatac tgattgtgac ttgaatttat
     3601 ttaagtttga agataaagaa ggtaaggaag tttttagaca tacatcagcg catatattag
     3661 ctcaagctat aaaaagactt tacccagaag caaagcttgc tataggacca agtatagaaa
     3721 atggattcta ctatgatata gatttagacc atagattagt tccagaggac ctagagaaaa
     3781 tagaagctga aatgaaaaaa atagctaaag aagatttaaa aatagaaaga tttgaacttc
     3841 ctagaaatga agctttagag cttatgaaag aacaaggaga agactataag gtagaactta
     3901 tttctgattt acctgaaagt gaaataatat ctttctacaa gcaaggtgat tttactgatt
     3961 tatgtagagg accacattta ccatctacta aaaaggtaaa agctgttaaa ttacaaagtg
     4021 tagcaggtgc atattggcgt ggtgacgaaa ataataagat gcttcaaaga atatatggaa
     4081 cttcttttga gaaaaacaaa gacttagaag agtacttaca tttactagaa gaagctaaaa
     4141 agagagacca cagaaaatta ggtaaagaat taggattatt tatgatacct gaagaaggtc
     4201 caggattccc tatgttttta ccaaaaggta tggaacttaa aaatgaatta ttaaaattct
     4261 ggagagaaat acacagaaaa gctggatata ttgagataga atctccaatc atattaaata
     4321 gaaaattatg ggagacttca ggacactggt atcattacaa agaaaatatg tatacagtta
     4381 aaatagatga tgaggattat gctataaaac ctatgaactg tcctggtgga ttaatttact
     4441 ataactctca actacattca tatagagatt tcccaatgag agttgcagag cttggtagag
     4501 ttcatagaca tgagttatct ggtgcattac aaggattaat gagagttaga gcatttactc
     4561 aagacgattc acatatattc atgttaccag agcaaattaa ggacgaaata aaaggagtag
     4621 ctaacttaat agatggtata tacaaaactt ttggatttga atataattta gaattatcta
     4681 caagaccaga gaactctatg ggaagcgacg aagagtggga agcagcagaa aatggattaa
     4741 gagaagcttt agaagagtta ggtctaccat atactattaa tgagggtgat ggagcattct
     4801 atggtcctaa gatagacttc catctaaaag attgtttagg aagaacttgg caatgtggta
     4861 ctatccaatt agatatgcag cttccaagac aatttgataa tacttatatt ggtcaagatg
     4921 gtgaaaagca tagaccagtt atgattcata gggttgcttt tggtagtata gaaagattta
     4981 taggtatatt aatagagcat tatgctggta aattcccagt ttggttatct ccaactcaag
     5041 ttaagatact tcctatatct gataaattta tggattatgc aaatgaagtt aagaaagagt
     5101 tatttgataa gggtataaga gttgaattag atgatagagc tgaaaaaatt ggatttaaga
     5161 taagagaagc tcagcttgaa aaagtgccat acatgttaat agtaggtgaa aaagaagtag
     5221 ctgataataa tgtttctgtt agatcaagag ataaaggtga aataggtagt attaaattag
     5281 atgaatttat agctagtatt tcaaaagaaa ttgaatcaag agaaagtatt attcaagact
     5341 aatatatatt atattagtag ctaagatatt gttatataag ataagtttaa tcaatatgtt
     5401 gatatgttga tgcatagtga ttaaatttat gaaaagatga gtataattag aggtacagtt
     5461 aatcatattt ttatgattaa tgtacctcta gttttttgta tggtagattt attaataaat
     5521 atatcataga agtgttttga acatggaaat atttatgtgc attctttaaa taaaagttta
     5581 ttttttgagt gataattaag tgcttgttgg taaggttatg gctgtaaaaa tcataacttt
     5641 attttcatat ttgttccatt ttcataaaaa ttattatccc tttaagaaaa tttcttaatc
     5701 ctcttgaaat atttcctttt tagtctaaag ttaaatttct ttcttcaaaa tgaatattta
     5761 aatgaactaa aatttaagta cattttctaa tcttatgagt ctatttttca tagataattg
     5821 ccctataata gaagaatcta tgttggtgct atgtggtgac aataacagca ttgtcagtga
     5881 ttaattaatg ctttataaat gattaattag tgttttatgt tatttttgga cttatcctct
     5941 atacatagat taatttattt aactttagat agagagagaa tgtcaagtac atacacagac
     6001 attagagttt aagaaagtgt ttatggagca agaactttta aaagaggtag tgacttagtt
     6061 taatttcaag aaatgataaa tacaaaagag gaaaaagaga ggtgttgaaa tgatagaaaa
     6121 agtagaagaa aaatctaaga ttattaaaac atcaaaagaa tataagcgaa ctataattga
     6181 agaaaaagat aaagaatgga ccaaaaaatt aaatgatata cttagtagaa gtagtttttc
     6241 taatgatgaa ttagataaaa tcaaggcatt aattccaaaa gagattttga ctaatgaaaa
     6301 tgttggagaa gttgtgactg aagatggata tgctaagcct gagtatgatg aggtatttaa
     6361 atcattgttc actttaaata atgaatatga tttacttgta aattttataa atgatattct
     6421 aaaagatgct ccatatgcta acaggaatat caaaccgttc actcaaataa agagaataat
     6481 tagagttgaa actgatccaa caattaatta cattggagag aaaagaccta aacttgatat
     6541 tttggctgaa gatgaagaaa ataatcatat aaatattgag atgcaaagag cttttgagaa
     6601 cgattatttg gaaagagctg aatattatct tagtcgtgta catggtagaa agttagaaga
     6661 aggtaaagag tataaagaaa ttggaaaaac tattggaata catatactaa atcatgtaaa
     6721 atataatcac atagaagatt atgtaaattg cctgagatta acaatggac
//
LOCUS       DAYAOX010000198         6732 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.199, whole genome shotgun sequence.
ACCESSION   DAYAOX010000198 DAYAOX010000000
VERSION     DAYAOX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.199"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            61..1185
                     /locus_tag="ACGI57_003055"
     CDS             61..1185
                     /locus_tag="ACGI57_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HFG9718111.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISTFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSDSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(1659..2483)
                     /locus_tag="ACGI57_003056"
     CDS             complement(1659..2483)
                     /locus_tag="ACGI57_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5780 domain-containing protein"
                     /protein_id="HFG9718112.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(2831..3886)
                     /gene="ispG"
                     /locus_tag="ACGI57_003057"
                     /gene_synonym="gcpE"
     CDS             complement(2831..3886)
                     /gene="ispG"
                     /locus_tag="ACGI57_003057"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFG9718113.1"
                     /translation="MSYERRKTREVSVGAVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(4069..5073)
                     /gene="rseP"
                     /locus_tag="ACGI57_003058"
     CDS             complement(4069..5073)
                     /gene="rseP"
                     /locus_tag="ACGI57_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFG9718114.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(5086..6240)
                     /locus_tag="ACGI57_003059"
     CDS             complement(5086..6240)
                     /locus_tag="ACGI57_003059"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFG9718115.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(6404..6607)
                     /locus_tag="ACGI57_003060"
     CDS             complement(6404..6607)
                     /locus_tag="ACGI57_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9718116.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         2235 a         1195 c          695 g         2607 t
ORIGIN      
        1 caatctttct tcaattgata tataatatgt ttatataaac taatgtatgt gaggtgaaat
       61 atgagcaaaa aaacaaaata tttttcttta attctctttc ctattgcttt gcttttaaat
      121 tttatagcca gtaaagttcc aaatattgtt gaaaaatatt actcccaatt catagataaa
      181 ataattgtac aaatattgag taaagtttct ggaattttcc cattttctct ctatgagatt
      241 acaatgtaca taatagttat atctaccttc ttatttttat gttgtacttt atctacaatt
      301 tttaataaga aaaaaaaatt aaacatatat ttaaaaaatt ccattttaaa tatattatcc
      361 attgtatcaa tagtctactt tctttttgta gtgctttggg gaataaacta taataggatt
      421 ccactagaaa ccacacttat aaataactat aatttaaaaa acaatacctc tatccaatca
      481 aaacaatata gtgttaaaga gcttactaaa ttatataaat tccttgtaat taaagcaaat
      541 gaaactagaa aactcacatt acaagataaa aatggtattg taaaatctaa tacagattat
      601 aatggtgtca taaatagagc tcaactagga tacgataata tcttggatat attacctggt
      661 gttagtgata gttattctaa gcctaagtat attatttcat caaatctaat gtgctataca
      721 ggaataactg gtatatactt tccttttaca ggtgaggcaa atgtaaatat agcaatacct
      781 gacttatata ttccttgtac agttgaacat gaaatggcac atcaaagggg atttgctagt
      841 gaagatgaag ctaattttat agcgtatctt acttctataa aacaccccaa tattgatttt
      901 aactattctg gatatatttt agctttaaat tatactgctt ctgctttaag taaggttgat
      961 tataatgcat atgttgatat ttctgctggc atttcagatt ctgtccgtag agatttaaaa
     1021 aatgaaagtg aattttggca aaaatatgaa ggtaaaatta atgaaatttc taatgagttt
     1081 aacaattctt acttaaaagc taatggcgtt actgaaggta ctcagagtta tggtaaaatg
     1141 gttgatttat tgctaactta ttatgaatta tatccatata actaaacact acaattcaaa
     1201 attttattca tatacagctg atattaaatc agctgttttc tatttaaata aattatttaa
     1261 cttcaagcaa tcttatataa tttgatagta cttatactat ctgtataaca tttcaattct
     1321 aactatttta gttaaaacat ttctattatc ttttttaata aatgttttaa ctaaaatttt
     1381 taaaacataa aacttccaca ttatttcact atctatataa aaatctaaaa attcacatat
     1441 aaaattacat aactaaaaaa tcataaattt atagaaaaca tatattcaaa ataaaaaagc
     1501 atgtatcaaa acttgacaca tgcttataat cttatgtata tatatcattt tgtcacaata
     1561 aaaatttact tgtaacactg atatttttaa acaaaatagt ccttatttaa ttaaattaac
     1621 attaattaaa ttcgtactca ttttgctatc attaaacatt acttatagta attttctaat
     1681 acactatttg aaacttcctt cccttgaaaa cttttcaacc aattctcata tgctgagttt
     1741 ttccatacac taccatcatt aaatgttact tgttttacac aagatataat gtactttaca
     1801 ccatgctctc cagttgattg tttccattta tcaaacaata gaaaggcatc attttgattc
     1861 ttacctggtg ctattgcatt ttgctttgca tattctttct ccatttgttt ccattctttc
     1921 aaccaatttt ctacccatgc cttgccttgt tgtttttcaa acatacttat tatatcctgt
     1981 ggtggtgatt gttgcatttt tctataagta tgtgaatatg attttggaga cacaggagat
     2041 atgcctgtaa caataccata gtctacacca gctgggctaa atccaacact tcctacttcg
     2101 ccatctgctg ccacattttg agcatcccaa tatagctcaa gtggttttcc atttttatca
     2161 tatgctaagc atgtaatttc ataatccata attgcctttt cactgctatt tgttaagctt
     2221 ataacattgc taggccaatg atatggagta tcatcatact gtattttgct attatttata
     2281 aataatggaa tattctcatt ataaacaaga cctttactac tttgaatatc tgataccttc
     2341 tttgtgacag tacttaccgt atttgctaag tatacatttg ttttttctga attggtcgta
     2401 ctaagagaaa atgttgttgt cgttccagta atcagcaaag caacaactaa aataggtaag
     2461 ctagatattt ttttatatct cacaattctt cacctctttt tctattaact tataataaaa
     2521 caatttacca aaacattaaa atctatgtta acataattta ccaactgttc tagtaactat
     2581 atcctatcat agttactatc aagtttcaat actattgatg gaaattttca ctatattaaa
     2641 tcttttttta attttataaa tttacaatat gatttaactc atacatatat agtcaaatta
     2701 gaatataagt ttcttacatt taatgaagtt tataaaataa aaaatgacta gataattaaa
     2761 tactgtaaat aaaaacttat tacaaaagta tttaaaaatc tagtcattta atatatatat
     2821 gaattgtata ctataatttt tcaatctctt ctagcaactc atcaactaat ttgttaccat
     2881 caatcattct aactatttca ccctttttaa ataaaagacc ttcaccttta cccccagcaa
     2941 tacctatatc tgcttctctg gcttcaccag gtccatttac agcacagccc ataattgcaa
     3001 cagtgatatt tttttccata ctaccaatct tttcttcaac ctcatttaca acactaatta
     3061 agtctatatt acatctacca catgtagggc atgatataac tttaatctta tcatttaaaa
     3121 gtcccaaact tcttaaaatc tgtttaccaa caataacctc ttcaacaggg tcgcctgtta
     3181 aagatattct aacagtatct ccaattcctt taagaagaag tgctcctacc cctatagaag
     3241 actttattgt tcctttgtga acacttccag attctgtaat tccaatatga agaggataat
     3301 caactttttt tgatattaat tcatatgcat caacagtttt ataaatatcg gatgatttta
     3361 gagatataac tatattatga aaatctaagt cttcaagaat cttaatatgt cccattgcac
     3421 tttcaactaa tgcttcagca gttgcagagc catacttttt taaaagttct ttctctaaag
     3481 aacctccatt aactccaatt ctaattttta aatttctttc cttgcatttc tcaactacca
     3541 tcttaactct ctctatactt ccaatattac caggatttat tcttattcca tccacacctt
     3601 gttctatagc ttcaagggct agcttataat cgaaatgtat atctgcaata attggtatat
     3661 ttacactaga ctttatcttt gctatatttt tagcagcttt aatatcagga acagcaactc
     3721 taactatatc acaaccagct tcctctaatc tctttatttg agctattgta gcatctgcat
     3781 ctcttgtatc agtatttgtc atagattgta tacttatagg attttctcct cctattttta
     3841 cagctcctac acttacttct ctcgtctttc ttctctcgta actcatattc cacctctata
     3901 tattatttta tattgaatct ctttaaatat taaaatatta taacctacat gtatattata
     3961 aattaaaaag ctatctagtt aaataagaat aaatttcata tataaaaatt aatttattct
     4021 tatttaaaac tacatagcct attttcttaa aatctctaaa aaactaaatc attgaaataa
     4081 tcttagtata tctttatatg tgataaaaag cataaataac attaaagctc caaaaccaac
     4141 aacatttatc atgccttctt tatttgggtc gagtttttta ccacctctaa cagcttctag
     4201 taacaacata agtattctcc atccatcaag tgctggtatt ggtaatagat ttactatacc
     4261 taaatttaag cttatgacag ctgctaaata cactacattt atgatgcctg tacgagcagc
     4321 atctgatacc ataccaataa cacctactgg tcctgctaca gcattgccaa tccctcctgg
     4381 tacacgtcct gtaatcattt gacctagaaa tgttagcatc tgttttgtca tatctacagt
     4441 agttttacat gcatttacta ttgaccctaa aaagtctttt tctctttgtg gttgaattcc
     4501 tatttcatac tttccattgt tatttttagg tgttacattt acaactttat cacttccatc
     4561 tctagttata ctaagtttta attctccacc agaagaagtg tttattatat ttgcaacttc
     4621 atcccaagtc ttaacagaat taccattaat tttatttatc ttatctccta cttgaagccc
     4681 tacagcctgt gcaggagtat ctggcattat ctttcctaat ttagtcgttg gagaacctat
     4741 atacaaaaat actggaacta aaagtattgc tgctaatatt atattgaata ttggtcctgc
     4801 tacaatagta ctaaatcttt gtaatataga cttgtttcca aaagaatttg gacttatttg
     4861 ttcttcatct tcaccttcca tgctgacata tccaccaatt ggaagtgctc ttatagaata
     4921 ctctgtatct ttttttacac tatatatctt aggccccata cctatagaaa actcatttac
     4981 tttgatacca cttctttttg caaatataaa atgtcctaac tcatgtatta atacaattat
     5041 actaaatagt attaatgcgg ctattatagt cataaattca ccttcctatt tttttatcag
     5101 atttgcaaca taagctctac tccatctatc tgtttctaag atttcttcta atgttggttc
     5161 acttatgcta ctatgaactt ctaaagtctt ctctatgtaa tatggtatgt catagaaacc
     5221 aattttatcc tctaaaaatt cacttactaa aacttcattt gctgaattta aaacagaaga
     5281 ataagttcct cccattttta aacactcata tgccaatttt aaacatggaa aagtttctaa
     5341 atctggttct tcaaaagtta aagtactaaa ttttgaaaaa ttcattcttt caaagctact
     5401 ttccattcta tctggatatg taagagcata ctgtattggt agtctcatat ctggacatcc
     5461 taattgagca attattgagc tatctgtata ctgcaccatt gaatgaatta tgctttgtgg
     5521 gtgaactact acatctatat tttcttgttc tactccaaat aaccatctag cttctatcac
     5581 ttcaagtcct ttattcataa gcgttgaaga atcaatactt atttttcttc ccatactcca
     5641 atttggatgc ttcaaagctt catttttagt tatatttgca agttctccct ttttctttcc
     5701 tctaaatgga ccaccagatg ctgtaagtat tatcttttct atattttttt tattttctcc
     5761 atttagacat tgaaatatag cactatgttc actatcaaca ggaagaatat ttacattgta
     5821 tttttctgct tctttcatta caagttcacc agcagttacc aacgtttctt tattcgctaa
     5881 tgctatgtct attcccttct ttatagcaca aagagttggg actaatccta tcatacctac
     5941 tacagcagtt aaaaggacat ccacttcatc aagtgatgat attattttta atccttccat
     6001 tcctgctaaa acttctattt ctatattttt aggcaacatt tcttttaatt ttaatgcttt
     6061 attttcctca aaaactgtta catatttagg tttaaactct actatctgtt ctagcaataa
     6121 ttcaatatta ctgttagctg atattgcaac tatctcaaac ttatctctat gttctctaac
     6181 cacatccaaa gtttgtttac ctatagagcc tgtagaacct aaaattgata tctttttcat
     6241 ataataatcc tttctaataa taaatatacc ctgtcaaaac attataacac tactaaaaca
     6301 attattttta ctattcatag aaatttcata tattattcca cagattaaaa ttaatctatt
     6361 attttttgta ttttgtggaa tattataaaa tattctagta ttattaaatg ccctttttta
     6421 tttccacaac atcttctatt ttacagtcta gtgctaaaca tatcttctcc attgtatcca
     6481 tagaaacaga tttatcattt cctagtctgc ctaaaacaga aatacttata cctgatatat
     6541 ttacaaattc cattttcttt atttttctgt caattaacaa tttccataat ggagcgtatg
     6601 aaatcatgtt atcacctcgc aaaatatatt ctatcacatt ttatctattt tggaaaattt
     6661 tcatctattt agataaataa tcacctgctt aagtgtcaaa taattttatc cttctaatat
     6721 gaaaatttaa ta
//
LOCUS       DAYAOX010000199         6559 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.200, whole genome shotgun sequence.
ACCESSION   DAYAOX010000199 DAYAOX010000000
VERSION     DAYAOX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.200"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            106..564
                     /locus_tag="ACGI57_003061"
     CDS             106..564
                     /locus_tag="ACGI57_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFG9718117.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(681..1418)
                     /locus_tag="ACGI57_003062"
     CDS             complement(681..1418)
                     /locus_tag="ACGI57_003062"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFG9718118.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            1635..3311
                     /locus_tag="ACGI57_003063"
     CDS             1635..3311
                     /locus_tag="ACGI57_003063"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFG9718119.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            3517..3717
                     /locus_tag="ACGI57_003064"
     CDS             3517..3717
                     /locus_tag="ACGI57_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1290 family small acid-soluble spore protein"
                     /protein_id="HFG9718120.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            3771..4934
                     /locus_tag="ACGI57_003065"
     CDS             3771..4934
                     /locus_tag="ACGI57_003065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9718121.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(5145..5567)
                     /locus_tag="ACGI57_003066"
     CDS             complement(5145..5567)
                     /locus_tag="ACGI57_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HFG9718122.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(5588..6424)
                     /locus_tag="ACGI57_003067"
     CDS             complement(5588..6424)
                     /locus_tag="ACGI57_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFG9718123.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
BASE COUNT         2500 a          788 c         1072 g         2199 t
ORIGIN      
        1 tatgagaata aattaaattt atttaaatgt cttgacaaaa aatgaattat caattacaat
       61 attatttaat aaagattatc aattgaaaat agtaagggtg atgttatggc aaatacaatg
      121 gatttattaa aagacaagtt aaaagaaact ggttttaaga ttacgccaca aagaagagca
      181 atagttgaaa tattgcttaa acatgaccat tcacatctaa gtagtgagga aatctatgat
      241 ttagttaggg ttgactgtcc tgaaataggc ttagctactg tatatagaac aatgcagcta
      301 ttggatgaaa taggattaat atcaaaacta aacttagacg atggatgtat aagatatgag
      361 attagtctac ataaagaaga ctgtcataat catcatcatc ttatttgtaa aaattgtgga
      421 aagataatgg aagctaaaga agacctttta gacaatatag aaaaagagat acaatcattg
      481 tacaaattta aaatacttga tcatgatgtt aaattttatg gactatgtga cgagtgtaat
      541 ggagtaagcg attcagaaga ataaacttct attgcttaat gtcgatggaa gaatagtata
      601 gaacttttat attctatgct attcttccat tttttattat aaaccatcta taaaatgcaa
      661 atatacagtt ttcaagatgc ttatagtttt aatattgaaa atataattaa tcctataagt
      721 acaaaaatga atgatttttc tatgaaatta ctaaatggtc ctccaacagt taaaaaacct
      781 aaagaaattt tcttttcgct tagcggataa aaaattggta ttccttgctt tgtaaataaa
      841 tctcctaaaa acatatgact tgcataacca atcgtaccat aaaaagataa attattaata
      901 ttagtgatta attctatttg ttttagtaaa aaatatacta ttatagttgc aaatatgcta
      961 tgagaaaaac ttctgtgctt taaccaggga aaaattgaca acattagaca aaaaattgta
     1021 aaatatattt caacttctat gatgtataag catatagcta atagtataaa tattaatgat
     1081 agaaaaactt ttcttagaaa tgtgtgggtt agttttgcct caataattat aattgcaaca
     1141 aatgtaatta ttgcgcttaa ataaaaatta tcatttatat ttattgaaat aaagaaaata
     1201 ataatattaa cagcatatat aaaaattcta tatatgtatt tggatacatt gttttttaga
     1261 atgaaatttg agaccataga attagattta tccaaatcgg gtagtacagc aaaagtagct
     1321 gaaactaaaa tatcaactaa agatatagga attttaaata ttaaagatgt ttggatagct
     1381 gttaagattc ctattgcaca atgagtttta ccacgcataa atcctcctaa attatccgat
     1441 attaaatcat ataatattta gaggatatca aaataaaata ggtttgtcaa aataatacgt
     1501 taaatgtatt ttaaatatag cttaaaatat gatacgaatt atggtacaat aagaataact
     1561 ggtagcgagt gcgtgatttg atattattta tgattgtaga acgtgttaaa aaattaaata
     1621 gacagaagga gatgatgcaa ttgttcaaaa agaacaccaa caagataaag gtaatggcct
     1681 tgggtggact taacgaagtt ggaaaaaata tgactgttgt tgagtataaa gatgaaataa
     1741 ttgttataga tgctggactt agttttccag aggatgaaat gttaggagta gatatagtta
     1801 taccagatat aacttattta gtaaaaaata gggataaaat aaagggtata tttattacac
     1861 atggacatga agaccatata ggagctcttc catatatatt aaagaaaata aatgtacctg
     1921 tatatggggc aagacttagt ataggtctta tacaagtaaa acttaaagaa cataaaatga
     1981 ataatgttaa gttaaatgtt ataggtccta gacaagttat aaagcttgat aatatggaag
     2041 tagagttttt gaaaaataac catagtatac cagatgctta ctctattgca atacacacag
     2101 accaaggtat aatataccac acaggagatt ttaagattga cttgactcct attgatggag
     2161 atgttatgga tatgcataga atctgtgagt tgagcaaaaa aggagttctt ttaatgctgg
     2221 cagatagtac taatgcagaa aaaccaggat ttactatgtc tgaaaagact gttggggtag
     2281 gtcttgacga attatttgca aaaggaaatg gcagaagaat tatagttgct acctttgcat
     2341 ccaacataca cagattacaa caaataataa atactgctga aaagtttaat agaaaagttg
     2401 ctatatcagg tcgctcaatg gtcaatgtag ttggagttgc aaaagaatta ggatacctag
     2461 atatttctga tgacatgctt atagatttaa atgatatatg taagtatgaa gatagtgaat
     2521 tagtgataat aacaactggt tcacaaggtg aacctatgtc agctcttgct agaatggctt
     2581 tttcagagca caaaaaagta gaaataaaga gtggcgattt agttataata tctgctcatc
     2641 caataccagg aaatgaaaaa ttaatatcaa gagtaatcaa tttcttattt gaaaaaggag
     2701 cagaagtagt atacagtgat atagctgata tacacgtttc tggacatgcc tgtcaagaag
     2761 aattaaaact tatacatgca ttagttagac caaagttttt tatgccagct catggtgaat
     2821 atagaatgtt aaaaagacat gctgaaatag ctgaacagct tggtatggat aaagaaaata
     2881 tttttgtcat gcaaacagga gatgttttag aattagataa aaattctgct aaggtagcaa
     2941 accgtataca aacaggaaac atattagttg atggtctagg agtaggcgat gtaggtaata
     3001 tagttttaag ggatagaaag catttatcag aagatggtct tatgatagtt gtagttacta
     3061 tttccaagga tgagggtaag gtcttagcag gtccagatat aatttccaga ggattcgtat
     3121 atgtaagaga gtcagaagat ttaatggatg gagcaaagga tataattaag aatgttctaa
     3181 atgaatgtga agagaaaaat attaaagaat gggcttattt aaagaataat attaaagaaa
     3241 acctaaaaga atatttatat caaaaaacta aacgaaatcc aatgatactt cctatcataa
     3301 tggaagtata atataaaaaa actataaatt tgaaaattag acctcttgta atttttaaag
     3361 taatatcaaa aatccaattg atattatctt aagaattaca agaggtcttt ttaatactca
     3421 ttttaaaaaa atatatatta cataaaatac caattataac aatcttttat tagtaaaaac
     3481 tacttatata taaattttac tgataggagg attcttatgg atgatgtttc aagacaaaat
     3541 gctataaaag ctttaaaaca aacaaaaatg gaaatagcag gagagtatgg aatgaattat
     3601 gaagatgctt ttgaaataat agaaaatgcg tctaataaag gtgttttaga aggttacttt
     3661 aaaaagcttg aaaagaaaaa aaatttaggt caaggtatat ctaggcattt agaataaaaa
     3721 aagttaaatg tgagaaaata agtttaaata aactttaaga ggtgacatcg atgaaaaaat
     3781 tagctagtac tgctttggct atattaattg cgcttactcc tttaagcttt tcttttgcaa
     3841 ataataagga aaatgctgat gccaaccaac taaatatatc ttcaaaatca gcaattttga
     3901 tggatgttgg tagtggacaa attttatatg aaaaggatgc tcataaaaag cttcctcctg
     3961 ctagtgtaac taaggtaatg actatgctac ttatagtaga agctttggat tcaggcaaga
     4021 ttaagcttga tgatgaagtt caagtaagtg aaactgcatc tagtatgggt ggtagtcaaa
     4081 tattcctaga gccaggagaa actcaaaagg ttgatacatt attaaaaggt atagctgttg
     4141 cttctgcaaa tgatgcttgt gttgctatgg ctgaacactt agctggaagt gttgaaggat
     4201 ttgttgatag aatgaatgct aaagcaaaag agttaaatat gaatgatact cattttgcaa
     4261 atacaaatgg acttccagtt gcaaaccact atacatctgc tcatgatata gctttaatgt
     4321 ctagagaact attaaaacac gaaatgataa gtaaatactt aactacatgg atggataagg
     4381 ttgtagtagg gaagaaacaa gttacagtag gtcttgcaaa tactaataaa cttataaagc
     4441 attatcaagg agcaactggt gttaaaactg gttttactca agaagcaaaa tactgtttat
     4501 cagcatctgc aaagagaggt aatactcatc tagttgctgc aactttagga gcagagactt
     4561 ctccagaaag atttaatgat gcctctagcc ttttaaatta tggatttgct aattatgaaa
     4621 gtgttaagtt atgtagtaaa ggtgataata tagcaacact tacattggat aaggctgatg
     4681 aaaacaaagt taaattggtt gctaaagaag atttaaatgc tcttattaaa aaaggaagtt
     4741 ctaaagaatt tgagaagaaa attgaaatag taaaaaatcc taagatgcca attaaaaagg
     4801 gaactgtttt aggaaaaata aagatatgta aagataaaaa agtaataggc gaagtggaat
     4861 tgataaatac taaggatata aataaggcaa gctatctaca aatgcttcaa agaataattg
     4921 ataatatgat ataaataatt atcagatgaa atataattat aatacacaaa gatatgatta
     4981 taatacacat aaaaaatacc cttatgtaat tatttgccat aggggtattt ttatttacca
     5041 tatagaagta ttttataatc ttatgatttt tatagaaata tagaaaagtt tatgtatctt
     5101 aaaatatcat ttgatttaat atcatatata gggttgttta acaattatct aagtaaaggc
     5161 atactaccag taagtttatt cactttcttt ttattcccat aaattgctat cccaaaatac
     5221 tgtaactcat cttctggaac actagcaatc ttttgtgtaa attcttcata aagattgcaa
     5281 ctttgtgcta catctgaaaa atctacaaca gttaaatcgt taaattccaa agtatataat
     5341 tttttacgta aatcttttat tatttctttg tttcctttta aaataggtac aggaatgtca
     5401 ataattccta agtggctatt acctgttttg tcaattacat ctgggccaac agtttctggg
     5461 gcatgttttc ctagtgtaat tcccattata gctgctgtat tggatataat tcccattggt
     5521 aaattttcgt ctattaccat gacacatttt gaatttttag tttccatttt tatttctcct
     5581 ttttacctta tttaaagttg tttgaatcat catttataaa tatattttga tactgctttg
     5641 gtgtaagacc aataaaattc ttaaaaaagt ttgtaaagtg actttggtct acaaatcctg
     5701 tttgtatcgc agcatctata ggttctacgc ctttttctaa aagcttttta gctttgttta
     5761 cccgtatatt ttctaggtat ctatatggtg taataccttt taatttcgta aaattacgaa
     5821 gtagagagta cttattcata cctgctatag tgcttaattc gtcaagtact atatgttcag
     5881 cataattatt ttctagataa tcacatactg cttgaatttc tatatttgta ttttccaagt
     5941 tagattgcaa atttggctct gtatgttctt caattaattg ttcaattagg aagaaaaaaa
     6001 gctcttcctt tttaaaatcc aattcttctt ccatgattat atagtgtaat tcttgtagta
     6061 gaggtactag ctcactgcga aatataacag gttgattaaa ttttggtaaa taatttttac
     6121 ctgtaatttc aaagacagtt tttttcatta tttctggctt aatattaata catctgtaat
     6181 caagtacttt atcatctatt tgttcacaag tatgactatc aaaagggtta aaaagtagca
     6241 aatctccagt acttgtggta tactctttat ttttacaaga taaatatctt tgaccttttt
     6301 ctataaaacc aattacataa tgttcatgaa aatggtttgg gaatttttgc attatgcctt
     6361 taaatctata tgcttcaatt tttaattccc tatcaaagca tactgtgcgt tcttcttgtc
     6421 tcacttaggt aacctccttt catgttatac aaagtgtaac acatattttt ttatacttct
     6481 tgtataatat tgactaagag acaataatgt caattcatgt caatataata ttacacttac
     6541 agtaggtgta attattata
//
LOCUS       DAYAOX010000200         6507 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.201, whole genome shotgun sequence.
ACCESSION   DAYAOX010000200 DAYAOX010000000
VERSION     DAYAOX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.201"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(370..777)
                     /locus_tag="ACGI57_003068"
     CDS             complement(370..777)
                     /locus_tag="ACGI57_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HFG9718124.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1251..2135)
                     /locus_tag="ACGI57_003069"
     CDS             complement(1251..2135)
                     /locus_tag="ACGI57_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9718125.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2426..2902)
                     /locus_tag="ACGI57_003070"
     CDS             complement(2426..2902)
                     /locus_tag="ACGI57_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HFG9718126.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3168..4343
                     /locus_tag="ACGI57_003071"
     CDS             3168..4343
                     /locus_tag="ACGI57_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HFG9718127.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4424..5227)
                     /gene="proC"
                     /locus_tag="ACGI57_003072"
     CDS             complement(4424..5227)
                     /gene="proC"
                     /locus_tag="ACGI57_003072"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFG9718128.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5267..5857)
                     /locus_tag="ACGI57_003073"
     CDS             complement(5267..5857)
                     /locus_tag="ACGI57_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9718129.1"
                     /translation="MVLNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLT
                     SVATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLK
                     EKTMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYN
                     SEKNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2149 a         1103 c          724 g         2531 t
ORIGIN      
        1 aagcacagac tatatcttat ccttcagcat tatccgttaa ggtctaccca cttccactac
       61 caatagcttg tagtgtactt ccctcaagag gaatagtcgt tgaagtttct cctattcgga
      121 gtttacctgc tgattgccca ttttaaagta cttaggattt aaccttatac catctaacca
      181 attttttcta ctttcgtaac tttcacactt aggtttattt catccttatg ttttagtttg
      241 attagcttta ggggtttcca gcaattcaag tagtattgga taggttctat cctatctcta
      301 catataggtt tccctatatg ctgactattt tagctactaa ctcatgacta aagtcacgag
      361 tgtgcgtagc tattttttaa tcaatatttt agtaaatttt acggtaagta aaaacctctc
      421 tccttcaaat cctatagcaa atatacttac aagttgcttt gagaaaataa ctcctaatat
      481 agctacaact atgcatatac caactattat atttaaaaca ttgtttaaaa actttagtgc
      541 ttgcttttca ccttgtttac tacttatatc ttgatacatt ggaataaaag tagtaactat
      601 agcagtacct attgctgcaa atataatatt tggtatattc atagctgtta aataactctc
      661 tgtatataag cctgtaccat aaattgatga taatactaat tctctaccca ttccaagaat
      721 cttagagatt atagtaacta tcatcaaata aaaagttgct ttagctacct tgctcatttt
      781 atcactccac ttctaacata cctcttttta atcaagtaaa atttagctca ttagatataa
      841 ttctatcaac taatttgcct tgcttagccc tatcaaaata taattaacac ctatttctta
      901 atatatcaca aactacatta ttcttcatat ttttttattt ctccaataag ttttacattt
      961 ttcctatata aattgtatag cttcaattat attcaaagaa atggagttgc tacccgataa
     1021 ctttaaaagt aaaaaacaga ttttattatc tttttaaact atattctaac atattttgtc
     1081 catattgaga aattttacta aactattatg tactttatta aataaataaa ataacagcac
     1141 acctataaaa tacaagtgtg ctgttcataa acagatttta taaaaaaata attcctaagt
     1201 agagattaac atgctttctc atactctcat atataatgtg tctaaaaata ttaataaaaa
     1261 tgctttaatt gttttaactt acttggatgt cttaattttc ttattgcttt agcttctatc
     1321 tgtcttattc gttctctggt aactccaaat acctttccaa tctgttctag tgttttaggg
     1381 tcatcatcat caattccaaa tctcattctt aaaacttgct gttcttgctc tccaagacct
     1441 gtaagtagtt cttctatctt ttcttttaaa ttatgctgtt caacttcgtg tattacaaca
     1501 tctttaaaat cagcatctga tggtataaat tcaactaaac tactatcctc atcttctttt
     1561 aatggagtat ccaaagatac aacattttta tctcttctta ataattccaa cactttgtca
     1621 acttctaagc cacatgcatc tgcaatctca tctatagttg gctctcttaa taatacattt
     1681 aaaagttctt gtttcttttt ctttacaaaa tttattctct gatgaagatg tataggtatc
     1741 ctaatagtat tttcgcaatc gtcgatatat cttgttatac tttgttttat ccaccaagtt
     1801 gcataagtgc taaatttgta tcctttttta taatcgtact tttctacagc ttttataagt
     1861 cctatatttc ctgcttgaat taaatccaac atatcaatac tatctctttt atatctcttt
     1921 gctatgctca ctaccaacct ataatttgaa tttataaatt tttcttttgc tactgaagat
     1981 gagttgatta tttccttagc cagctctacc tcttctcctg ctgacaacat tttgtattcc
     2041 tctatttcct ttaaatacat tttcatcgca ttcgatacat ttgatgaacc cacacaaata
     2101 taattttcat aattattttc ttttttagta tccataatca aaccccttct attcatagaa
     2161 tttgctaaaa caaaaataaa taatttaaac ctacataatc tacaattaca taatatccca
     2221 ttctttaaat gaattgtttt gtatattatg tatacaaatt tataaaaact atacctataa
     2281 tattattcta cttctaacag caatatcctc cttatatata taatttattt tattatatgt
     2341 tgttatattc ttagtttttt gaatgatata tgtctaaaaa aagactgttt actaaacagt
     2401 ctttttttat atatatgctc aatatttaat tttcatgatg atgattacat ccacatccac
     2461 aattttcatc atggtgatga tgttcttcta aatcttttac ctgttgttcc atttcatgaa
     2521 actcttttac cattatatca taagttggaa gagctgcctc agtatcgtat tcttcccctc
     2581 tccaatcagc gtacatcata gcatctccat tagttaaaac caatctacct gacatagtta
     2641 tattatccac ttcttctaaa aattctattt gtttttgtaa aacttctcct tctaactcca
     2701 catcttcatc taaacattgc actatcatta caggtgcata ataatcttct tcatctttaa
     2761 catttacaac aatatctaaa attccttctg caccttcttc aaattctgcc aactcaacat
     2821 ttgtatcaat catatcttga ggtactaata aatcttctat taaatatttt attactctat
     2881 cttttactaa ttcttttgac atttattttt ctccttccga ttctataaca ttatcataat
     2941 catttctata ttatagtata ctacattaat tgttttttca atctcttaca aaaattattt
     3001 tttattatct tccttatatt ccctttttta ttacaaagtt gtaatcgcat tgatttaaaa
     3061 aatagtttat atttagtata atagaaatgt agcacatatt tttattcaat atattaataa
     3121 aaaaatatat tgaaaatgta atgtatgtta aatctaggag gaatattatg aagtttaaaa
     3181 aacaaattag ttctattgtt gtgtctacta tgcttgtact tgggagtatg aacctttctt
     3241 atgcagacgg tacaaatgta gttacacttg gagctaactt atcagagtct caaaagcagc
     3301 aaatgcttaa ttactttgga gtaaaaaaag accaagtctt agttttagat gtaacaaatg
     3361 cagaagaaag acagtattta caaggtgttg caacagaggg tcaacttgga aaagttacta
     3421 tctcttgctc atacgttgaa cctactaaaa aaggaaatgg aattaatgtt aaaacagcaa
     3481 acttaacttg ggtaactagt tcaatgattg cttcaaccct tacaacagct ggtcttgaag
     3541 atgcaaatgt tattgctgct gctccatatc ctgtaagtgg tactggagca ttgacaggaa
     3601 tcatgaaagc atttgaggat gcttcaggaa agaaacttga tgaaactaag aaagaaattg
     3661 cttctgaaga attggtagtt acaggtgact taggagatga tatagggcaa gaaaaagcaa
     3721 caggtgtaat gaatgatata aaaacagaaa ttgtaaagaa tggtacaaaa gataccaatc
     3781 aaatagcaaa tacaataaat aatgttgtaa ataattacaa tattactatt acaccagaac
     3841 aaaaagaaca aattaaaggt gtaatgaaaa aaatagctga ccaaaattac gattacaata
     3901 gcatgaaaaa tacactagat aatgtatcag ataatgtaaa tgaacaatta aagaaattag
     3961 gtgaaagtgt taagggttct ggtatactag atactattgg tggatggttt agtggaatag
     4021 gagactggtt ctctggttta ttctcaggcg gagataaaaa agatttggga atcttaaata
     4081 atactaatga tgagtctctt ggctctgatg ctgtaataaa ttctacagaa ggtgttcaaa
     4141 taaatccaca aaataatact aattctaaca atgatagtga tacaaatagc tcaaaaaatg
     4201 atacttcaag taataatgaa aataacgata gttctcaaac aaatgaaaac aacacaacaa
     4261 ataattcaga aaatcaatca aataataatg actcagttgg tttctttgaa aaaataaaaa
     4321 attggctttc aggcttattt tagaaattaa acaaataaaa agaattgcct aaaatggaaa
     4381 ttttctataa aaggcaattc tctttattta caataaattt ttattactca ctcatttttt
     4441 tagatttttc tatacatgca catattgctt ttatcacaga agctctaaaa ccttcttctt
     4501 caagtacttt aactgcttct atagtagttc caccaggtga acataccata tcttttaatt
     4561 caccaggatg ttttcctgtc tcaagtacca tttttgcaga acccataaca gcctgtgatg
     4621 caaacttata tgcttgtggt cttggcattc ctgcaattac tgctgcatct gctatagctt
     4681 ctataaataa aaatacatat gctggtgcag aaccacttgc accaattaca gcttctataa
     4741 gatactctgg tactacctca gtatttccaa aagaattaaa tacttctgta actgcttcta
     4801 agtcttcttt gtttatattt ttatttatcg aaagagaaga catggcttca tttacaagag
     4861 ctggagtatt tggcatagtt ctaactattt ttttatcttc tcctataata gactctatat
     4921 cttttataga ttttcctgct gctattgtaa ctataatttt attgtcatct ataaagtctt
     4981 ttatctcttc caaaatatca tcatatatat ctggtttaac agctacaatt actatatcag
     5041 aggttctagt tacttccttc gagtcttttg tagtatttat tccaaaattt gcatctattc
     5101 tatcgagagt tccttgacta tatgctgatg ctataaccat attaggttca actaattttg
     5161 aatttactat tccccctatc atagcgcttg ccatatttcc tgcacctatg aatccaattt
     5221 ttttcacttt agtacctcct tattgactaa tagtaatcgt taaaatttaa gcttctttaa
     5281 ctgcatcaga tttttcaaat ggatttggta tacttactac caaaattttc actaccttat
     5341 tagtatagtt gacccaatta tgattgttct tttcactatt atagtgtatt gtgtctcctg
     5401 gatacatttc tatttgttca tctcccaaaa acactgtaag agtaccctct aatacataca
     5461 caaattcctc accttcatgt acgtaacaac aaatttcctc actactctta cttggaagaa
     5521 tttcaattaa tcttggtagc attgtttttt cctttaaact agatgataaa tgataatgta
     5581 taaatgttga gttttctaca tcaaatactt ctttttcata acttcttaat acagctcttt
     5641 tatgctcttt tggcttcata aaaaagtaag ttaattctac atctagtaca cttgctattt
     5701 ttcctaagga atctgttgcc acacttgtta atcctctttc aagttgtgat aaaaaaccaa
     5761 ttgacaagtt tgttttttca ctcatatcct tcagcgtcat ttgcttttga gttctgagtt
     5821 gctttatttt tgctcctatg tccttattca aaaccatcac ccctaaataa ttttaatttt
     5881 ttcataatat aaatacaaaa atataaatat aggtactgaa cccatcttta tttcatttta
     5941 aaaaattttt ataagtatta ctataattta tcaatctttt tttgtctaca tttttacgat
     6001 tatttacgat ttatttttca catatgtata tttaatcttg ccaactaaag atacattttt
     6061 tataagcatt tactctttat ataataaaac ttaagttgtt catgtagtct tcttctggtt
     6121 taattattaa aatttcaaat tatattatta tttctacaca ttattacatc ctttagtaca
     6181 attataacat aattttacaa tttaatgata tctattcaat gtcttttttt actctacaac
     6241 atattttatt ttagagttta atactatata tattttttat atacagcctt gtcatttttt
     6301 agattgtcaa gagttattga agtattttat gtagatttag attttatttc ctaatttctc
     6361 atttaacaag aattgcttat gataaaaaat aatatcatct caataagatt cttgtaaata
     6421 tatctatatt ctaaatataa tttctttttt tgtcaaaatt tcttcattct ttaataatta
     6481 atttattact tataaaataa actaatt
//
LOCUS       DAYAOX010000201         6487 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.202, whole genome shotgun sequence.
ACCESSION   DAYAOX010000201 DAYAOX010000000
VERSION     DAYAOX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.202"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            59..697
                     /locus_tag="ACGI57_003074"
     CDS             59..697
                     /locus_tag="ACGI57_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9718130.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            829..1491
                     /locus_tag="ACGI57_003075"
     CDS             829..1491
                     /locus_tag="ACGI57_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9718131.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            1491..2672
                     /locus_tag="ACGI57_003076"
     CDS             1491..2672
                     /locus_tag="ACGI57_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9718132.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(2853..5513)
                     /locus_tag="ACGI57_003077"
     CDS             complement(2853..5513)
                     /locus_tag="ACGI57_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718133.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPVFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5510..6193)
                     /locus_tag="ACGI57_003078"
     CDS             complement(5510..6193)
                     /locus_tag="ACGI57_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718134.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
BASE COUNT         2283 a          988 c          887 g         2329 t
ORIGIN      
        1 atatttacat aaatatacca tcagtatact tttttatcaa tagaaaggaa taatttatat
       61 gtcagaatta tcaaaaagag ataaagaaaa aatacaaaga gaaaatgaga ttattgataa
      121 ggcagaaaaa ttattttgtc ttaatggatt tgataataca actatgaatg aattagcaaa
      181 agaagttgaa tatactaaac gcaccatata caagtacttt tcatgtaaag aagatttatt
      241 ctttgctgtt gtcttaagag gatataaaag actttgggat aatgttaaaa tagaaagtgc
      301 aaaaggcaaa acagggtttg aaaaaataaa actttcatac tttgcttttc ataaatttta
      361 ctctgtagaa ccttctctat tatgccttat gggaatgatt ggtattgtaa actctgaaaa
      421 atcggatact gatatgctgt ttaaagaaaa gtttttttct tttaataaat ttatgtttga
      481 tgaaatacaa ggaatgtttg aaattggaaa aaatgataga agtattaggc atgatattga
      541 aatacctact cttatgtatt catcaatatt tacacttaca ggatttttta atttactatc
      601 tgtaacagga aaatcatacc tgaacaactt taatatagat gaggaaaaat ttattgaaac
      661 aactcttgct cttttaatag attcaattaa agcataaacc taagtttata aattaaagtt
      721 tatatactta aatgataatt ttttctatct ctaatatgaa aaatattaaa cataaaacta
      781 ataacttagt taatggaata actaaattac tgaaaggatt aaaagtaaat ggctaaaata
      841 cttcttttag aagataatga tacaattaca tttggaataa aaacttccct tgaaaagaaa
      901 aatcacactg tatgttgtta taatacgatt gctgaagcta aaaaaaattt tgataaaact
      961 attgatttaa ttctgcttga tttaaatcta cctgatggta ttggatacga gttttgccaa
     1021 tatgttaaaa gtatagaaga tactccagtg atatttttga cagtccgtga tgatgaaaaa
     1081 gacattatac gtggattaga tatgggtgca gatgactatg tggttaaacc gtttttgctc
     1141 tcagtacttc aatccagaat taatgcagtt cttagaagaa ctcattcaaa tactaacaat
     1201 actattacat gtgaaaatat aagcttgaat aaggttgaag tcaaagcata tttagataat
     1261 gttgaagtac ctttgacagc aggagaatat aaacttcttc taagtcttat ggaaaataaa
     1321 aatagagcta tgactcgtac aaagttacta gaaatgctct gggatgtaga tggtaatttc
     1381 gtaaatgata acactcttac tgttacaata aaacgtctta gagaaaaatt aaaaaatcca
     1441 ccttgtataa aaacattaag aggcatagga tatagaatgg aggacaacta gtgaaaactc
     1501 caagaaatat caacagtaca gttgttttta taatattctg cttaattgta acttttttat
     1561 tgagtgtaat tctaacaact ataacttctt ttaatgaata caatgtactg tctaaggctc
     1621 ttggtgcatc atatgagtac aatcctaagt ctacagaaag tatactaaaa tcactgaaaa
     1681 attctagtga taaggatttt aaaacaggaa atgaaatttt atcaaaacat ggatattcta
     1741 aaagtacatt ttggagtaaa aacttttttt attttctagt gatatcgttt ggtataattt
     1801 caacaatggc tacagttgta tatataatca aacatagaac aaataagcaa aaactgagta
     1861 gaataaacaa tcttactcaa tacttagaag aagtgaattt aggaaaagag tctgtacttt
     1921 tacgatgtga agatgaattt tctcaccttg aagatgagat atataaaaca gttggggaac
     1981 ttcgttgttc aaaagaattt gctttaaaag aaagacaaac tctttcagat aaccttgcag
     2041 atattgctca tcaacttaag actccaatta cctctatgtc acttatggca caattacttt
     2101 cagaaaattg tccagatgaa gaaatagatt atattaacag acttacaaat caaatctcaa
     2161 gacttgagag actagtttca tctttactta cattatcaaa attggatgca agtactttaa
     2221 tatttgaaag caaacttata gatgtacatt ctcttcttac ttatgcaatt gagcctatag
     2281 aaggttcttt aagagaaaaa catcagacat ttactatcga ggcttctcct tcggtagaat
     2341 ttgaaatcga tattaattgg actttagaag ctcttttaaa tattcttaaa aattgtagtg
     2401 agcatataga agatggtgga tatatagttg cttattatag tcaaaatcct ctatatgttg
     2461 agattatcat agaagataat ggtaaggggt ttgctcagga agaacttcca tatattttta
     2521 atagatttta tcgtggtaaa aatgctagta aggacagtat tggtataggg cttgctctat
     2581 caaagtctat tatagaacgt caaaatggta ctctacatgc agaaaattgc cataatggtg
     2641 gtgcaagatt cataattaaa ttttacacat aaaatgctgg attataggta aaatacctat
     2701 aatccagcta tatctatatt ctatgttatc tattgtttta gtaatatact tctatttaca
     2761 tttaaggttt tattaaacaa acaacttaac tatccctata cttgtaagta gtcttaaatc
     2821 gaaaccttta aataagttca aatagatttt gtttatattg tttcattttt tatagattct
     2881 actatatttt cattcttcaa ctgcttgtat cctgtcatat atgatgctat tacaataaaa
     2941 agtatcaata tagtaaaacc tattattgta gatataggtg caaatggtaa atactctata
     3001 aaatatattt catttatacg tagaaaagca tacactattc ctatttgcac aggaatacta
     3061 agtagtaatg gtgtaattcc taagaataaa ccttccaaaa taagcatctt ttttatgcct
     3121 tctggagata atccaacaga acgcaacata gcaaactctt gtcgtctact tctaatattt
     3181 cctgacactg tagcaagtac atttgataat ccaatcatag ccaataaacc tgtaataaag
     3241 cctactatta aattcatggt ctttattcca ctagcctttt gtttttcact ttctaaaaca
     3301 tcctcaatac tataatctcc tgaaccatag tctttattat aaatggactc taatttttct
     3361 cttactctag gaatgtcctc acgattattt actttaaata ctcctatcag cctatttgaa
     3421 cgtaacctac ttacttcatc ataattttta gatatttcct gcaccataga cattggaact
     3481 atttgcatta atgtatactt tgaactagta tctactattt taggaagtgt gcttgtaata
     3541 gctcccactt tcatgttgta agtataatcc cctttatctt cgtcatatac tttttcattt
     3601 aatttaatgt tatctcctat tgaaagattt aaataatcta tatatacttt atttctacgt
     3661 gtactacgat taatatcttc ctccttatta tatacaattg aggttggatt ttttgtatca
     3721 taaaataact ttgggtctac agaaatttgc ctacaatatt ctgtaaaact cttatcatca
     3781 agcccaatta tatttgaaat tatacgaaac ttgccatcac gttctatagg agagtacttt
     3841 ttagatgaag ctatattttt aaatcctcca tttttcgcaa gttcttttga agcatctttt
     3901 tcagtcaacc aagtggaagc agacattttt tgataatata gtgaactttt tacaccttct
     3961 gtattatcta atttttcaat gaaagcagac tctacaggtt cactattttc caaataaact
     4021 gctatatctc tttggtcctt ataattatca cttccataaa cagcttttgc tccttcttga
     4081 tttgcaatta tatgcaaaaa acatgtaagt aataaaaaag ataatgttaa agatattgtt
     4141 gcagtacgat aagatttttt acgagcacgt agtgcgtttg atgcaagttc tccttctatt
     4201 ccaaatatct ttcctaatga atatttatgc gactttttga ttttaacact atctccttgt
     4261 cgtattgctt ctataggaga tatctttgat acttttcgtg ctggtataag tgcagaaatc
     4321 cagactgtta ctattgtaag taaaactgct ggcagaaaaa caggaatacc ataagtaaat
     4381 accacttctg gaacatcagt atgataatta actgaattaa cataaactat aagcctatta
     4441 cacaataacc atcctaaaaa taaacctatt ggtaatggta taattgtcag taaaaagcct
     4501 tcaaacacta cagacctttt aatttgtttt ggtgatgcac caacactaga taatatccct
     4561 aattgagtaa gtctagcatt tgcagacagt gaaaacgcgt tgtaaatcac cattacaaac
     4621 acggctactg taaaaattgc tattgtaaga tacataatag gagttgaaaa ttttgatata
     4681 ctactcatag atgctttctg ttctggtgaa aagactaaca tctttaaaag ataattacta
     4741 ttgtatttaa gattgtattt tccatattca ttagtcttcc atcccaatga tttagcaagt
     4801 tttggaagtt ccttatacgt gtctcttata tttttaaatc gcaagtacac agttatacta
     4861 tcattaggtt ttatacttgt tttatcaaga tatccaagag ctgtatatgc aggtattact
     4921 gatgaagtag aaacatctac ctttcctact attgtcaatg tagcctctga aattttttta
     4981 aaactttccc ctttcgattt tggggctatg ggttcaagag gctttccatt taggattcta
     5041 tctcctattg ggagagtaat tttatcacca atttttacac ttggattatt ctcaaaatat
     5101 tgttttgata atgcaatttc attagcatta tttggtcgac gaccctctat tatagtatcc
     5161 ttttctggca tagaattcca atactctgat gtagcatttc tataaattat ataatctctt
     5221 ttctcatcat taattttgcc tacataccat tgtcctttta ccattacatc atcaactgaa
     5281 gaaaaatttt caattaatgg taagtcactt ccataagtat agtcaaacaa ttcaccatgc
     5341 cagttacctt ctttctcaat tgaaagtttt ataaaatctg tccacatggt atacaacagt
     5401 ccacacaaag aagacatcat ggttgtagta agtaatatag ctaacattat agctatacta
     5461 ctacgtttat tgcgtcgtac aaagtctaag gtgtattctt gaagaatatt catttcttca
     5521 ccaccttttc agagacaatc actccatctt ccatagttac aatacgattt gcctctagtg
     5581 ctattttctc atcatgagta ataagaagta ttgtctgatt aaatcgttta tttgataagt
     5641 ttaacaagtc tacaatttct tctgaatttt ttctatcaag attaccagtt ggctcatctg
     5701 ctaataaaat agctggtcta tagattaaac ttctagctat agataccctt tgttgttgtc
     5761 cacctgaaag ttgacttgga agatggttta atctatcaga taatcccaat atagatacaa
     5821 tctcagagaa tctatcctca tccacctttc ttttatcaag taacatgggt aataatatat
     5881 tttttctaac atcaagggta ggtataagat tataaaattg ataaataaga cctacttttc
     5941 tacgtctaaa aactgcaaga gcttcttcct ttaaacttga tatttctgta tcttcaacat
     6001 aaatctttcc agatgtaggt ctatctactc ccccaagcag atgtaacaat gtagatttgc
     6061 cagaaccaga tgctcctaca atggatacaa aatcaccttt ttgaactgat aaatttacat
     6121 tattaagggc tgtcactctg gtttgatttg aaccatatat ttttgtgaga ttttcacatt
     6181 ttaatatttc catatacaat catctccttt atggttaaat tttagcaacc taaaatgaca
     6241 ttatagtgac atgaatacaa atataaatta ctattaaaaa tattgtttag tttttgcaca
     6301 ttatttaaaa ttataatata aaacaaaaac tatgcaatat attttattta tacttttaat
     6361 atattacata gtctttaaaa ggtattaact ttaaatctat aggtattcaa tttaataatc
     6421 aatagcaagt attttatgta ctaaaggagt tttgctctat atagactcaa tttagtaaaa
     6481 ttattta
//
LOCUS       DAYAOX010000202         6474 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.203, whole genome shotgun sequence.
ACCESSION   DAYAOX010000202 DAYAOX010000000
VERSION     DAYAOX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.203"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(264..2651)
                     /locus_tag="ACGI57_003079"
     CDS             complement(264..2651)
                     /locus_tag="ACGI57_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFG9718135.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKAYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2674..3039)
                     /locus_tag="ACGI57_003080"
     CDS             complement(2674..3039)
                     /locus_tag="ACGI57_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFG9718136.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3238..4086
                     /locus_tag="ACGI57_003081"
     CDS             3238..4086
                     /locus_tag="ACGI57_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718137.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGIPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            complement(4523..5611)
                     /locus_tag="ACGI57_003082"
     CDS             complement(4523..5611)
                     /locus_tag="ACGI57_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718138.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5736..6464
                     /locus_tag="ACGI57_003083"
     CDS             5736..6464
                     /locus_tag="ACGI57_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9718139.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
BASE COUNT         2260 a         1089 c          710 g         2415 t
ORIGIN      
        1 tttttatgaa tctaatttaa acttaatata aacttattaa taaaccttct atgtctaaat
       61 tttaaaatta caatataaaa gctctacact aaataaatac attaaataaa cataatggat
      121 aatgaatata aatttatata tgcaaagcgc tccaataaaa aggtaccaat aaagttaaaa
      181 gaaacttatt gatacctttc attattacat acttcaatat ataattataa gttttttaaa
      241 tagtatggat ttacatttat cccttatctc atagctctca tagcatttac tactgctata
      301 agtgccacac ctacatcagc aaatatagct tcccacatag tagctactcc tccagcacct
      361 agtatcataa ctataatttt tactcctaga gcaaatatta tgttttgcca cactatctta
      421 ttagtcttgt ttgctatttc aattgcttta gatatcttgc taggctcatc tgtcataagc
      481 actacatctg cagcttcaat agcagcatct gaacctaagc ctcccatagc tataccaaca
      541 tcagcacgag ctaatactgg agcatcattt attccatcac ctacaaatgc tattttctct
      601 ttttcagttc ttccttcata taaatcttct aatctatcta ccttttcatt aggaagtaaa
      661 tttgagtaaa ctgtatccaa ttctaattct tgagcaatat tttttgctac tttctcatta
      721 tctccagtta acattacaac ttcttttacc cctatttctt ttaagcttct tatggcattt
      781 ttactatctt cttttacttc atcagagata actatataac ctctatatgc tttatcaaca
      841 gcaatataga ctactgtgcc tacttcttta gctgatgaat aagatatatt ttcttttttc
      901 ataagtttct cattacctgc taatataaaa ttgccattat gctttactct aattccataa
      961 gctgctattt cctcataact gtctattgta tctaaatcaa tttttttatt atagtaactt
     1021 aatatggact ttgctattgg atgatttgaa tttacctccg caatagctgc atattctaat
     1081 aattcttctt ctgatatacc ttcaggattt aacttagtta cattgaacac acctttagta
     1141 agtgttcctg ttttatcaaa tactactgta tctacacttc ttaaagcttc taaatagtta
     1201 cttcctttaa taaggatacc atttttagat gcaaacccaa ttccactaaa gaaactaagt
     1261 ggtattgata aaactaatgc acaagggcaa gaaacgacta agaatatcaa tcctctatat
     1321 aaccaatcac taaatacctc acctgagatt acaagtggtg gcacaaatgc tattaataaa
     1381 gcagctatta ctactattgg agtatagtat cttgaaaact tagatataaa gttttctgtc
     1441 tttgattttt taatgctcga attttcaaca aggtcaagaa tcttagaaac tgttgattct
     1501 ccaaaactct ttgtaacttc aatactcaat aaagcatttt tatttataac acctgataag
     1561 atttcatctc ctttttcaac ttctctcagc actgactctc ctgtgagtgc agaagtatct
     1621 aacatagaaa ctccatctac tactacacca tctaatggta ctttttcacc aggttttact
     1681 actattatat cacctacatt tacctcttct ggtgacacca ccttaacttc agaattaact
     1741 tttagatttg catagtctgg tcttatttgc ataagtgaag ttatagactt tctggattta
     1801 ccaactgcaa caccttgtaa atactctcct actttataga aaagcataac ccctactgct
     1861 tctgataact cccctattgc taatgctcct actgttgcta gagccatcaa aaagttctca
     1921 tcaaatactc ttccttttga tatatttctt attgctctta aaagcacatc accacctact
     1981 attacatagg caactataaa tactatattg ctaaactgac tttcataacc tgtagctgtt
     2041 tgatatatac caaatatata tacaagtgct ccaattataa gtggaattaa ttctttctta
     2101 tttgtatcat ttacagctcc tgatttttca gtagttctcc cttttacttt gtcagtagac
     2161 cctacttgta tatcaagccc tggctctgtt gaatttatta tttcttttat tttatttata
     2221 acatcatcct cttcaaagtt attacttata tttactgtaa gttttttatt tacaaagttt
     2281 aaattagaag attcaacttc ttttaattta gaaactttgt tattaatctc ttctgcacaa
     2341 tgagcacagt ttaatcctcc taatataagc tcttttttaa ttgatgtttt actagctgca
     2401 ttttctttag aacttatttg tatatcaaga cctggctctg ttgaatctat tatatctatt
     2461 atcttttcta tagtagtatc ctcattaaaa ctttccttta tatttactgt aagtttttta
     2521 tttataaaat ttaaattgga agatttaact tcttgtagtt tagaaacttt attattaatc
     2581 tcctctgcac agtgagcaca atttaatcct cctagtataa attctttttt aattgaatta
     2641 tttgcttcca tatatattca ctcctctcat ttatcattta taagtctctc taatatgatt
     2701 taatccacaa tcaaatatct gacttatatg actatcatct aatgagtaat acactgtttt
     2761 tccttctctt ctaaacttta ctaatctagc ctgctttaat actctaagtt gatgagatat
     2821 tgcagaatga gtcatattta ataaactcgc tatatcacat acacacattt catttgcaaa
     2881 taatgcatat aatatcttta ctctagtagt atccccaaat actttaaata attctgctaa
     2941 atcatataac atttcttcat ctggcatagt tgatttagcc tctgttacta tttcttcatg
     3001 tactatatta caattacaat catttatctc ttctctcatt tttaatcctc cattatttta
     3061 atatatgaat aactgttcat atgttcatta tatgttatca tttctaaatt tgcaacaata
     3121 atttttatta atttaatata aatagaatgc acatattata aactttttta atatatatat
     3181 tattagaata agtttttgta aactttttta tagttttcta ggaggtgcaa gtttaatatg
     3241 agtaacaaga aaaaaaagga tttagatact agttacatgc cttacttaga cccagcgaac
     3301 tatgcagatt atattactaa tgaagaaatt ccccaagatg aattgagaaa tgaccctcaa
     3361 atttctaatg gttatacaaa aatacctaat aaccagaaca taaataacct atatcctaat
     3421 aatgtaaatc attatcctaa aagtagtaat tctccaaaca acttgcctaa cacaaataat
     3481 acatccggaa atatgaatta caatacatct gctacaaata atatgcctaa taacatgaat
     3541 aataatacgg gtatgcctaa taatatgtct aacaatatga atagtaatat gggtatgcct
     3601 aacaatatgc ctaataacat gaatagtaat atgggtatgc ctaacaatat gcctaataat
     3661 atgaatagta acatgggcat tcctaacaat gttcctaata acatgaatag taatatgggt
     3721 atgcctaaca atatgtctaa taatatgaat agtaatatgg gtatgcctaa caatatgtct
     3781 ggcaatatga ataacaatat gtatggtaat acaggtatgc ctaataacat gaacagtaat
     3841 atggggtata atatgaacaa tactatgggt attcctaact tctcatgtaa tcaaaatatg
     3901 cctcctaatg tacttatgat gataccagga gttatatgtc ataatacaat gcaaggtatg
     3961 cctgtagtta tgcctagtac tatgcctcct aatatatatc ctactcctta tggttctagt
     4021 aatatgtcta tacaaggtat accacaggct actaatattg aggaatttga tgaggaagaa
     4081 atgtaatata tattttttta agtaagttgt ataaaacatt aataattgac cataataata
     4141 aatgtattcc tcataatatt cccccaatat tattataggt atagaaagtt gactgaatat
     4201 gcgcttattc ggtcaacaga tattaacata atctaataag ataaacatag aaaaagtcag
     4261 ataaaattta attttatctg actttttcat ttaaaaatgc tctcttgtat tggtgggtca
     4321 gaatagtatg atactcgtat ttgagactaa tattggcaaa caacataaag aaaaatataa
     4381 attttttata ataaactgta taaaatttta ataattgacc acaataataa atgtattcct
     4441 cataatattc ccccaatatt atagaaataa atttaagcga gattaaattc agttaatctc
     4501 gcttaaataa tgtctaaatt tattattcca aaaaatcatc aaaatctaat gaacctgatt
     4561 ctgcaaaatc atataatgca ttttgctcaa agaaaaattt ctcttcaaat ttaatatagt
     4621 cgctataatt agattgactt atttcattga tataattgca atcattttcg ttataagtac
     4681 caataaaaaa cttatttccc attaagtaat atatagcttt catatcatta tttattatat
     4741 atacattttc ttttacatgg tcattattaa aattaaatct agtaaagaat ataccttcat
     4801 tcatattact ttttaacaga aatggatttt ctctataaaa tactcctgca aattcttcag
     4861 gtgaatttac actgtaaaca cttacaaatt cacttttaga aatctcatca aatacctcaa
     4921 aatcatacat aggttcttta tctgtcacta atagagaatt aattctaaaa ccagtttcat
     4981 aacacttact tatgcaaaaa gcaattttac atatataata tccatcatta tcttcatata
     5041 aagcttcact tatatatctg ccttgacctt tatcacttac cacgttttta agtaatgttc
     5101 catttatatt agtaatgtgc atatttgcaa tctcgtcact tccagaaaag tactttaaac
     5161 tttctctatc ccatgctata aatctcatgg tcatatagtt tataaattca acttcctcgt
     5221 caactctttt acatatttta ttgtataatt tttctatagt taaatctgtt ttatagtttt
     5281 ttataatatc aatatactca catttatctc ctggcaatag cttatcataa taaagattct
     5341 tattaccaaa ataagatact aaaaataaag attcatcctt cgttaattca actctatctg
     5401 aaccaaatcc tcccatcaat ctttcttctt ccatataagc ttcttcttgt gttgggttat
     5461 ttaaactcac ataatccgct aatcctaatc cctctgcatc taataaaaaa tattgacaaa
     5521 agtgattgtt ttcatcatct atccaattta ttataagacc taaactgccc ataagtctag
     5581 aatttgtagc ctttgcaaaa ttaaaattca tatagtcaca tcctttaaac tctaaaatac
     5641 ttgtttttta atatataaat tattatacat atattttaac aacttagata caatttataa
     5701 tataatataa ggaaatgatt aattgaggtg gtcttatgaa aaatatagca gctttttttg
     5761 atatagatgg tactctttat agagactctc ttatggttga acattttaaa aaattaatta
     5821 aatacgatat aattgaccaa aaaaattggt ataaacatgc tagagacact tttatggatt
     5881 gggataaaag acaaggtaat tatgatgatt atctattaga aatatgtgac ctttatgttg
     5941 attcactaaa aggattagat aaaacctgta tcaactttac aagtgaccaa gtaattaaat
     6001 taaaagcaga tagagtttat aaatacacac gttcaaggat tgagtggcat ctaaatcaag
     6061 gacacatagt tatatttata tctggtagcc ctggatttct tgttgaaaaa atggccaaaa
     6121 aatataatgt aaccgatttt ttaggaagtg attatgtctt tgaaaacaat atatttacag
     6181 gcacagttat tccaatgtgg gattctataa gtaagaataa tgctataaac gattttgttg
     6241 taaagtatga tttagattta tcaaactcgt atgcctatgg agatactaat ggtgatataa
     6301 atatgctaaa aagagttgga aatccaatag caataaatcc aacaaaggaa cttttatctc
     6361 aaatatcact tgatgaggaa atttctaaaa atgttgagat aatagtagaa agaaaagata
     6421 ttatttattc tctatctaca gatgtaaata tagtggatat ttaatttata ctat
//
LOCUS       DAYAOX010000203         6452 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.204, whole genome shotgun sequence.
ACCESSION   DAYAOX010000203 DAYAOX010000000
VERSION     DAYAOX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.204"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            331..1524
                     /gene="nifV"
                     /locus_tag="ACGI57_003084"
     CDS             331..1524
                     /gene="nifV"
                     /locus_tag="ACGI57_003084"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HFG9718140.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1549..3474
                     /locus_tag="ACGI57_003085"
     CDS             1549..3474
                     /locus_tag="ACGI57_003085"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HFG9718141.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3496..4491
                     /locus_tag="ACGI57_003086"
     CDS             3496..4491
                     /locus_tag="ACGI57_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HFG9718142.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4816..5289
                     /locus_tag="ACGI57_003087"
     CDS             4816..5289
                     /locus_tag="ACGI57_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9718143.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5323..5817
                     /locus_tag="ACGI57_003088"
     CDS             5323..5817
                     /locus_tag="ACGI57_003088"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9718144.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5914..6435
                     /locus_tag="ACGI57_003089"
     CDS             5914..6435
                     /locus_tag="ACGI57_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9718145.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
BASE COUNT         2606 a          664 c         1291 g         1891 t
ORIGIN      
        1 gaatgtaact taaattaaat tttagtagga attttatagg tttattttta aaggtttttt
       61 gaaaataaat ctatttaaaa attaaattaa aatgcaagaa aaacataata actttcattt
      121 ttaagaagtt ttcacaaaaa agaaaaaata tagtgcaata aatgatacaa agtaatataa
      181 tagattacaa gatatgaaaa gctaaaaaaa taaattgcaa atgaaattaa aatgaataaa
      241 aaataaaaaa ataatcataa gttagaagta tcaatgaaaa ataagactta atgtttttaa
      301 aattaaaagt atactaaggg gggcattaat atgtgcgtta tatcaaaaga tagagcaaag
      361 gaaataaaaa tagtagatac tactttaaga gatggagagc aaacagctgg ggttgtattt
      421 gcaaatagag aaaaaataat gattgctgaa atgttaagtg acctaggtgt tgaccaaatt
      481 gaagttggta tacctactat gggtggagat gaaaaaaatg taataaagca catatgtagt
      541 agaaatttaa aatcagatat aatggcttgg aatagagcag taataaaaga tgttgaagaa
      601 tctatttctt gtggtgttga tgcagtagct atatcaatat cagtatctga cattcatata
      661 gaaaataaat tgagaacttc tagaggctgg gttttagaaa atatggctaa aacagttgaa
      721 tttgcaaaga aaaatggatt atatgtttca gtaaatggag aagatgcttc aagagcagat
      781 atagacttct taactgaatt tataaatgtt ggaaaacaag caggggcaga tagatttaga
      841 tactgtgata cagtaggagt tatgaatcct ttttctataa agaatgcaat agaaactctt
      901 tatgaaagaa ctaattttga tatagaaatg catacacata atgactttgg gatggcaact
      961 gcaaatgcac ttgcaggaat agcagcagga gcaaattatg ttggtgttac agtaaatgga
     1021 ttaggtgaaa gagcaggaaa tgcagcatta gaagaagttt taatggcact taagtgtgtt
     1081 tataaatgtg atttaaataa tatagatact agaaaattta gaggaatatg tgagtatgta
     1141 gcacaggcat caggaagaat acttcctaca tggaaaccag tagttgggga taatatgttt
     1201 atacatgaat ctggtataca tgcagatgga gctttaaaag acccacataa ctatgaacca
     1261 tttgacccaa gtgaagtaaa tcttgaaaga aaaatagtaa taggtaaaca ttcaggaaga
     1321 gctgcagttg taaataagtt gagtgaatat gaaatgtata tttctccaga aaacgctacc
     1381 aagctattaa atgctataag agctacatct ataagattaa aaagaagttt aatggataaa
     1441 gaaattcttc aattatattg tgatatacta gcacatgaga aaggcacaac agaagaagaa
     1501 gcagttagag gttcatatat ttaagatata attgtttagg aggaaaatat gggagataat
     1561 atagtttata aaataattaa aaaacatata gtagatggag aagctgttgc tggaagttca
     1621 attggtataa aaatagacca aactttaaca caagattcta ctggaacaat gacatatctt
     1681 caactagaag ctatgggaat agataaagta aagactaaaa gaagtgtggc atttgtagac
     1741 cataatatgt tacaacaagg atttgaaaat gcagacgacc ataagtatat acaaacagtg
     1801 gcagataaat atggagttta tttctctaag ccaggaaatg gtatttgtca tcaagtattt
     1861 ttagaaagat tttcaacacc aggagataca cttttaggtt ctgatagcca cacacctact
     1921 gctggaggtg tgggcatgat ggctattgga gctggtggat tagatgttgc tttagccatg
     1981 gcaggaggag catactatat aaaagcacca aaagtctgta aggtgaatct agttggaaaa
     2041 ttaaataata tggtatcatc aaaagatata atattagaag tgttaagaaa acaaactgta
     2101 aaaggtggag taggtaaagt ttatgaatat ggtggagaag gtgtcaaaag cctatctgtt
     2161 ccacaaagag ctacaataac aaatatgggg gctgaacttg gagcaactac ttcaatcttc
     2221 ccaagtgatg aaaaaacact agaatttttt aagagtcaag gaagagaaga cgcatgggta
     2281 gagctaaaac cagatgcaga tgcagtttat gatgaagaga ttactataaa cttggatgaa
     2341 ttaaaaccat tagctgcaaa acctcatagc ccagacaatg tagatgaagt tgaaaatata
     2401 ggcaagatta aaatagacca agtggcaata ggaagttgta caaattcttc ttatgaagac
     2461 ttaatgaaag tagcacaaat cctgaaagga aataaagtac ataaggatgt gagtttggta
     2521 atagcaccag gttcaagaca agttatggag atgattgcta gaaatggggc tttggcagat
     2581 ataataagtg ctggagctag aatactagaa aattcttgtg gtccatgtat tggtatgggt
     2641 caatctccag gtactgactc tgtgtcttta agaactttca atagaaattt ctatggtaga
     2701 agtgggactt tgtcagcaca agtatattta gtaagtccag aagtggcagc agtaagtgca
     2761 atcaagggag tgcttacaga cccaagagaa tttgatatta aatttacaaa tttagatgta
     2821 aatgagtttt tgatagatga tagcatgata attaaacctg ctgatgtagg aagtgatgta
     2881 gaagtagtta gaggacctaa tataaaacct ttccctctta atacagagct aagtcagtct
     2941 ataggtggaa aagttatatt aaagacagaa gataatataa ctactgacca tataatgcca
     3001 tcaaatgcta agttacttcc ttttagaagt aatataccgt atcttgcaaa ctattgtttc
     3061 aatactgtag atacagaatt tcctcagaga gcaaaagaca ataatggtgg atttatagtt
     3121 ggtggagata attatggtca aggttcaagt agagaacatg cagctttagc accattatat
     3181 ctaggtgtta aaggggtaat agttaagagc tttgcaagaa tccacaaggc taacttgata
     3241 aatagtggta taattccaat ggagttttgt gatgagaaag attatgaaaa tatatctctt
     3301 ttagataatc tggagatacc aaatatttta gacaatctag gtagtggaat tttagaagtt
     3361 aagaatacaa ctaaaggaac ttcttttaag gtcaaagtag aattgtcagc aaaagaagtt
     3421 gatgtattaa aagctggtgg aaaattaaac tacactaaaa atcaagctaa ttaatatgaa
     3481 aagagggaat attgaatgta taaagtgaca cttataccag gagatggaat aggaccagaa
     3541 gttgcgaaag ctatgaaaaa ggtagtagaa gctactggag tagaaataga atgggaagaa
     3601 gttaatgcag gagaggcagt tattgaagag tatggtactc cacttcctga atatattata
     3661 gatagtataa aaaagaacaa aatagctata aaaggcccaa ttactactcc tgtaggtaaa
     3721 ggatttagga gtgtaaatgt aactttaaga caggcattag atttgtatgt caacttaaga
     3781 cctattaaaa gttttaaggg gataaaatca agatatgaag atgttgattt agtggttgta
     3841 agagaaaata cagaagattt atatgctgga atagaacata aaataggtga ttatgcagct
     3901 gaaagtataa agataataac aagaagtgca agtgaaagaa tcgttgattt tgcatgtaac
     3961 tatgttaaag ataataagag aaaaaaagtt acagctatac ataaggcaaa tataatgaaa
     4021 atgtctgatg gattgttttt agatgtgttt agagaagttg catcaaaaca tggtgtagaa
     4081 tatgatgatt tgattgtaga tgcagcagct atgaacttag ttttaaatcc agaaaattat
     4141 gatgtaatgg taatgccaaa tctttatgga gatatactat cagatttagg tgctgggctt
     4201 gttggaggtt taggtataat tccaagtgca aatattggta aagattgtgc gatatttgaa
     4261 gctgttcatg gaagtgctcc acaaatagca ggtcaaaata aggctaatcc aacagcactt
     4321 attcaatcat cagtaatgat gcttagatac ttaggtgaat atgaaaatgc acaaaaaata
     4381 gaaacagctt tagaaaaagt atttcttgaa ggaagtaaat tgactgtaga tttaggtggc
     4441 tcagcttcta ctacagagtt tgcggatgag gtgtgtaagt atattgtata gattttattt
     4501 agggaaaaag taaaatatag attgaagaaa agaggctttc tcaaaataga gataagcctt
     4561 ttttattgga atgataaaat atatttgatt ttatacaaaa gagagttcct cataaacgaa
     4621 aaagttaatt ttgggatatc cattttaatt ttaaaattaa ttgattaata cgcaaagaaa
     4681 gattaaataa atttattatt tttaattgaa ctttcaacca aaatatggta aaatttaaaa
     4741 agttgaactt tcaactaaaa atgaaaatat tgattatgtg aaattctata aaacagaaac
     4801 tatttgactt tagccatggt agtttcagtg gaggtgtgtt tgattaagac tttagatagt
     4861 aatatattaa gagaagtagg tactttatct agagcagtaa attcaataaa tgatattaag
     4921 tacaaggaat taaagttaca aaaaggacaa tttacatttt tgacaagaat ctgtgaaaat
     4981 cctggtataa atcttgtgga attatcaaat atgttaaagg tagacaaggc tacaacaacc
     5041 aaggctatac aaaaattaat aaaggcagga tatgtagata aaaagcagga taagtttgat
     5101 aaaagaggat ataatttaac tccaactgat aaatctttgg aagtttatga gttgataatt
     5161 gaagaagaga atagaagtat agagatatgt tttgataatt ttacagatga agaaaaacaa
     5221 gtggtaacta agttattaga aaaaatgagc aaaaacgtag aaaatgaatg gtttaaagta
     5281 aagagatagt aaataaatac attaacttaa ggagatattt ttatgattag aaaagcgaat
     5341 atgaatgatt tagaaagcat tatgaaaatt ataaaatcta cagtggaaga aatgaagact
     5401 tataataata cacaatggga tgaaaactat ccattagaaa aagactttgt ttcagatatt
     5461 aagaaacaag atttatatat ttatgaagtg gatggagaag tagcagggtt tatatgtctt
     5521 aattatgaag agccagaaga atatttaggc ttaaattggt catctaataa aaaagcaatg
     5581 gtaatacata gaatggctgt aaatccaaac tttagaaaga caggaatagc atctaagtta
     5641 gttgattttg ctgaaaaatt agctgtagaa aacaatgtat catacttaaa gagcgataca
     5701 tattcaataa actcaaaaat gaatttatta cttacaaagt gtggttttat aaaaatcgga
     5761 gaaatgagtt ttttaggaaa ggagaaatct ttctactgtt atgataaaat attataaaga
     5821 gcatttataa tacaaggggt aaataataga ttttcagcat tagtaagggc ataatataac
     5881 ctagtatatg ctattatgaa agggatgatt tatatgattg gatttctaaa gaaaagaaga
     5941 agtattagaa aatacaagga tgttgaagta gaaaaagaaa aattagataa aatattaaaa
     6001 gctgctcttt tagctccatc atcaaagggg cttagaacat gggaatttat agtggtagat
     6061 gataaagaaa agctaattaa tttatctcaa tgtagaacta aaggtggtgg atttttctta
     6121 aaaaatgcac ctttagctat tgtaattatt gcagataaag aaaaaaatga tgtttggata
     6181 gaggatgcaa gtatagctgc ttcatacata caacttcaag ctcatgaact aggtttaggt
     6241 tcttgttgga tacaagtaag aaatagaatg catgatgata atatagaagc tgataagtac
     6301 attagagaag aattaaaagt acctagcaag tacagtgtag aatgtataat ttctatagga
     6361 tatcctgatg aagaaaagaa agcatacggt gactctgatt tagactataa aaaggttcat
     6421 tttaacaact tttagtatat aatatgttgt at
//
LOCUS       DAYAOX010000204         6425 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.205, whole genome shotgun sequence.
ACCESSION   DAYAOX010000204 DAYAOX010000000
VERSION     DAYAOX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.205"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..285
                     /locus_tag="ACGI57_003090"
     CDS             <1..285
                     /locus_tag="ACGI57_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFG9718146.1"
                     /translation="NRKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQIC
                     NKCGYKNEEVKDLNIREWIYPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            717..971
                     /locus_tag="ACGI57_003091"
     CDS             717..971
                     /locus_tag="ACGI57_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718147.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1007..1330
                     /locus_tag="ACGI57_003092"
     CDS             1007..1330
                     /locus_tag="ACGI57_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HFG9718148.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1604..1756
                     /locus_tag="ACGI57_003093"
     CDS             1604..1756
                     /locus_tag="ACGI57_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718149.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2025..2873
                     /locus_tag="ACGI57_003094"
     CDS             2025..2873
                     /locus_tag="ACGI57_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFG9718150.1"
                     /translation="MWILVPIITIVLLIIAVSSMQYILVMIAFLLIIYSFIEKKIVMG
                     LVSVLFFTYSIYLCATWEDKSLIADNKVETVRAQRETVEREKEMERRRIQEEVDKERY
                     IEKHGMEISENDLKVKLEALVPQEYKGKKYELKVGKFKRYSMYFDLTVQNEKFSNSEE
                     CKKFVKEIANDLKKIKISKAYFKFHSKDDGGIYNSVYIDYFRNIQNNVDNVENLEFNE
                     FELKTEEEEKREQEKIEQEKNSYNNYIQNRVVDPLDRIKKLKELLDSGAITQEEYNKK
                     KKELLE"
     repeat_region   3140..3955
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3140..3168
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4110..4298
                     /gene="srl"
                     /locus_tag="ACGI57_003095"
     CDS             4110..4298
                     /gene="srl"
                     /locus_tag="ACGI57_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Srl"
                     /protein_id="HFG9718151.1"
                     /translation="MNKIATKSRAEYMKNCRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            4418..4783
                     /locus_tag="ACGI57_003096"
     CDS             4418..4783
                     /locus_tag="ACGI57_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFG9718152.1"
                     /translation="MKNELMMFEGKEIEVFEFEGRILFNSKDVANCLDIKNVNENIIL
                     MNEKQVVKLRNSDISNTDIRKLNNAGENFLTESGVYKLIFKSRKEEAERFQDWISDEV
                     LPSIRQTGAYITNNVIPKS"
     gene            4836..5231
                     /locus_tag="ACGI57_003097"
     CDS             4836..5231
                     /locus_tag="ACGI57_003097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718153.1"
                     /translation="MLKELLDDAGFDNKSKLLTAKTLYKKAEIDLPIEINEEEHYFDT
                     KQIASKLKIYSKSNKPAQMAVCEIIKKIDLEDGEVKGVWEINGSWTGTVNKYTKSVID
                     KVRTWIEENNRPTKIAGEKKNYYVFYKIE"
     gene            5283..5444
                     /locus_tag="ACGI57_003098"
     CDS             5283..5444
                     /locus_tag="ACGI57_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718154.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5772..6236)
                     /locus_tag="ACGI57_003099"
     CDS             complement(5772..6236)
                     /locus_tag="ACGI57_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718155.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKNKILRERKYKKRR"
BASE COUNT         2611 a          610 c         1107 g         2097 t
ORIGIN      
        1 aatcgtaaac tatctcgctc aatttctgat gtgtcatggt cagagtttat tcgtcaatta
       61 gaatacaagg ctaattggca tggtagacaa attgtaaagg tgggtaaatt ctttgcaagt
      121 tcacaaatat gcaataaatg tgggtataaa aacgaggaag tgaaggactt aaatataaga
      181 gaatggattt atcctagttg taatgaaact cacgatagag atataaatgc aagtataaat
      241 atattaaaag aaggattaag actaataaca attcaaaata aataacgtat gtaagaaccg
      301 taggaactac ggggatagcc tgtggagaat tagtaagaca tatgttagta tgcaaaattc
      361 tgtgaagcag gaaacctgtg actttagtca tgggaggttc aggctagtat tactcattat
      421 tgtattcaaa agctaaagtg gaaacctagt gaatatatga atttagaagt taatgagaga
      481 gcattagcag ccgactcaat acttattaaa attgaagatg aagaagaaac aatgaaagaa
      541 gctgaaagag agaaaaagag aggtagaaga aggtagaatt taaagttaaa atagttacat
      601 tgctaatata tgaaaatgtt acaaatttgg aaatagatgg atatatatgt tgtatagtat
      661 aatgtagata atatatcacc aagtaaaaaa caacaaataa atctaaatta tgaggggtgg
      721 aaaataaaga tattaaactt acaatatgta tatttgcttt tgtaattgga ataatagtgt
      781 tattttgctg tataggtcta ggtcaattta atatggattg tattataaaa caaaatggtg
      841 ggtcaatagc taccgaacaa tacaacatat atttagaaca atctatttca caatatagaa
      901 attttggttc tatactggtt ttactaggag gaataggaat actgtttgat aaaagtgtca
      961 gaaaatacta aaaaagtata tttttattta ggatgtgatg aatgatatgg caagagatgt
     1021 atttagtgta aagatgacac cactagaagc acagaaaatg gtagaaaaaa atttggaagc
     1081 agatttagtt tctgttgata tttataacct agaaaataat aagtctataa tagttacaat
     1141 ctttgaaaaa tattatgcaa gaagcaatag tgatgcaggt ttggtagtga tttgtgaaaa
     1201 tactacagga aaaacacttg ttaaaattac ctcaactggt agtgcaattg gactatttca
     1261 gattgattgg ggaacaggta ataatttgat taaaagagta aaaaaaatac ttattgatta
     1321 tattatttaa ttattttatg tagatattct taggttggta tggaatttgt actaacctat
     1381 taagtaatca ataaaactaa ataaacatag aaagcactta caaatatgta ggtgcttttt
     1441 attgaaaaaa tagaaaataa aataaaaagt aagatatagg aaaaatatgt aagaattata
     1501 tgttataata attgtagcaa gaagatgtaa tctacaattt atagagtgga gttcatgtat
     1561 taaaaaatta tcctcccaac gataagaagg gaggtgaata tacatggata attttttatt
     1621 taatgtgtta gctagtgtaa cagctagtta catagtttat ttgattagca agttaatcaa
     1681 aaaagtaaaa agccactcta ccgcaaagag tgacttaaag attgatataa attttaaata
     1741 tcatcgtaaa aaataacttt cagttataga acttcactct accgcaaata gattgtagtt
     1801 cttcttgttt ttattatacc acaaattaga aaaaatattg catataatat tttttatagt
     1861 caataaaaat atgaaatttt ttatacacaa tgatgatgaa tctaactaga tgaataaatt
     1921 taattgtaaa taagaaagta cttacttaaa tggtaggtgc ttttgttttg ccaaattatg
     1981 ctataatatt gagtgagatg agtaatttgt gggggaattt tataatgtgg atattagtac
     2041 caataataac aatagtatta cttataatag ctgtttcttc tatgcaatat attttagtta
     2101 tgatagcttt tttgttgatt atatattctt ttatagaaaa gaaaatagtt atgggtcttg
     2161 tgtcagttct ttttttcact tattcaattt atctttgtgc aacttgggaa gataaatcct
     2221 tgatagcaga taataaagtt gagactgtta gggcacaaag agaaactgta gaaagagaaa
     2281 aagaaatgga aagaagaaga atacaagaag aagtagacaa agaaagatat attgaaaaac
     2341 atggaatgga aatatcagaa aatgatttaa aagttaagct agaggcttta gtacctcaag
     2401 aatataaggg gaagaaatat gaattaaaag taggaaaatt taaaagatat agtatgtatt
     2461 ttgatttaac tgtacaaaat gaaaaattta gtaattctga agaatgtaaa aaatttgtaa
     2521 aagaaattgc taatgatctt aaaaaaatta aaataagcaa ggcgtacttt aagtttcatt
     2581 caaaagatga cggcggtata tataattctg tttatattga ttattttaga aacatacaaa
     2641 ataatgtaga taatgttgaa aatttagaat tcaatgagtt tgaacttaaa accgaagaag
     2701 aagagaagcg agaacaagag aaaatagaac aagaaaaaaa cagttataat aactacattc
     2761 aaaacagagt ggttgaccca ctagatagaa taaagaaact aaaagagttg ctagattcag
     2821 gagcaattac acaggaagaa tataataaaa agaaaaaaga attattagaa tagataatat
     2881 agtaagcact tacaagtatg taggtgcttt taaatttaca aagtattcca ttttaatttt
     2941 atagtttaga ttttatgata taataaaaat atagaagttt tgcagtgtgc gatatttgtt
     3001 acaaagtagg gcttaatact tgaaatctaa gatgttgagg gtgcgtgata agtgttatca
     3061 attgcactat tgcccgctca ctgcaatttt aagagtattg tatatatgta ggtattggaa
     3121 atgctaagtt tattttgggg ttttagatta actatatgga atgtaaatat tacatgggta
     3181 aaaattttga tgatttttat gacagtttta gattaactat atggaatgta aattgtatag
     3241 aattaagaat ttttttaata ctaattatag ttttagatta actatatgga atgtaaatat
     3301 tacatttaat ttgcattgct ttgatgactt ctttgtttta gattaactat atggaatgta
     3361 aatgcgtaca aggttgagaa gctccgcaat ctcaacgtcg tgttttagat taactatatg
     3421 gaatgtaaat atttgaaagg aataaatttt aaatgaatga aaggataggt tttagattaa
     3481 ctatatggaa tgtaaatcag ccaaaaactt cgaatttgaa ctaaaatgcg tgggttttag
     3541 attaactata tggaatgtaa attctcatga ctattgaata aatataataa acaagcatac
     3601 gttttagatt aactatatgg aatgtaaatt tagaatcaaa agcacttaat cctagttctt
     3661 tcatagtttt agattaacta tatggaatgt aaatagcttt ttagcttcgt ctactacttt
     3721 ataagcaaag cgttttagat taactatatg gaatgtaaat gatgaacaat aaaacaatca
     3781 tctaaagacg aagaaagttt tagattaact atatggaatg taaatgttcc tgttgtgttt
     3841 tttactaatc ccattttata tgttttagat taactatatg gaatgtaaat tgctgtgatt
     3901 tcactaataa aacaattaat aattgtgttt tagattaact atgtggattc aaaattaaat
     3961 aaacaaagaa agcacttaca aatatgtagg tgcttttatt ctgctcaaaa ttggtcggtt
     4021 gggtaaaatc attagaaaaa attagtaaaa acctcttttc tgtaactcgt tacaatatta
     4081 ttattaatgt aacgagttac agaaaagagg tgaataaaat agcaactaaa agtagggcag
     4141 agtatatgaa aaattgtcga aaagataaaa gaggttttag tgtactttta gacaaagaaa
     4201 agttagataa atttgatgaa gtattagagg aaaagaatct aaccaagaaa gaatggctag
     4261 aagaaaaaat cgacgaggaa ctggaacaaa aggaataaaa aataagggtc actcccaccg
     4321 accaaagttt gagtaacccc tatgatgtat actatcgtat atcaattata atatatgtca
     4381 ttccttaaaa aaatcaatta ttaaggagtg taatattatg aaaaatgaat taatgatgtt
     4441 tgaaggaaaa gagattgaag tatttgaatt tgaaggaaga attttattca actcaaaaga
     4501 tgtagcaaat tgtttagaca taaaaaatgt taatgagaat ataatactta tgaatgaaaa
     4561 gcaagttgta aagttaagaa attcggatat cagtaatact gacatccgaa aacttaataa
     4621 tgctggagaa aattttctta ctgaaagtgg agtgtataaa ttaatcttta aatcaagaaa
     4681 agaagaagct gagagattcc aagactggat aagcgatgaa gttttaccaa gcataagaca
     4741 aactggtgca tacataacta ataatgtaat cccgaaaagt tgagagaaaa agcaagtgag
     4801 attgaaaaat tacagttgct tataacagta catctatgtt aaaagaattg ctagatgatg
     4861 caggctttga caataaatca aaactattaa cagctaaaac attatataag aaagcagaaa
     4921 ttgatttacc aatagagata aacgaagaag aacattattt tgatacaaaa caaatagcat
     4981 ctaaactgaa aatatattct aagagtaata aaccagctca gatggctgtt tgtgagatta
     5041 ttaaaaagat tgatttagaa gatggtgaag ttaaaggtgt ttgggaaatt aatggttctt
     5101 ggactggtac tgtaaataaa tatacaaaga gtgtaataga taaggtgaga acttggatag
     5161 aggaaaataa tagacctacc aagattgcag gtgagaagaa gaattattat gtattttaca
     5221 aaattgagta aatttatcag ttgtattaaa taatttagtt tagttttggg gggattaata
     5281 caatgtgtga gaatttactt gataatatgt atattgaaaa gagaaaagaa gaatatagaa
     5341 ttaaactttt gaaaataaga gaaacagata tagatatata taataagtta gaaagtatag
     5401 tatataaact ttctgagaaa aaattagaga aaaataataa atagacatat aaagcacttg
     5461 gatattctac tgtttcaagt gcttgtttgg tataaaatgg tataatggag atagagaatt
     5521 atattaacca aaaaatggag aaaatagaga agatgattta gttgacgctt tatcagtagg
     5581 agtacctgca ggtaagacag aatctctagt acgattagtt ggagatggaa taagtgatac
     5641 tcaaaaaagt ttaataaata aaagagaatt tagaaatata actcaaattg gtggaaatgg
     5701 aaatgagaaa gcatttagtg aagtagaaaa cttagttaaa tagattagaa ggtagcaact
     5761 agagttggtt gttaccttct ttttttatac tttctctctc taagtatttt atttttcata
     5821 tcttctaata catctaatgc agtagtataa aattgtatat tatttctata aggtatagct
     5881 attggaagaa tgcccttgtt aactaaaaaa ataagaagta caataaataa aagtgtcata
     5941 atcattctaa taaaataatt agtgatatct gataaatcaa atgttaagtt aattgttgta
     6001 gaaaatacaa cagccataat agatatatac aaagcaaaaa tggggttact atttttttgt
     6061 aactgtcctt taagcctaga ttgctctatt tctatatctt gaatactttt tatggaatcc
     6121 atttcactat aatacttata aacttttaaa taatattttt tatacttgtt taaagcttct
     6181 tcattatctt taaggtcttt gtcacttaac tctgatttaa cttccccaaa atccataaaa
     6241 tccctcctac aatgttatat aatatacttt aatttatagc atatttaaga acttatatca
     6301 gcaatttgtc gaacgatttt agctaaaaat agagtataat gataggctta ttatttataa
     6361 gatattattt tttatatagt tatatatttc tttctaatta gataatataa tgctgttgtt
     6421 ataat
//
LOCUS       DAYAOX010000205         6424 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.206, whole genome shotgun sequence.
ACCESSION   DAYAOX010000205 DAYAOX010000000
VERSION     DAYAOX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.206"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(52..1488)
                     /locus_tag="ACGI57_003100"
     CDS             complement(52..1488)
                     /locus_tag="ACGI57_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HFG9718156.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            complement(1698..3098)
                     /locus_tag="ACGI57_003101"
     CDS             complement(1698..3098)
                     /locus_tag="ACGI57_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFG9718157.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            complement(3125..4471)
                     /locus_tag="ACGI57_003102"
     CDS             complement(3125..4471)
                     /locus_tag="ACGI57_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HFG9718158.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNIIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEKAYSIFYGDILESCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            complement(4839..5513)
                     /locus_tag="ACGI57_003103"
     CDS             complement(4839..5513)
                     /locus_tag="ACGI57_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9718159.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKAEELADWISEYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
     gene            complement(5574..6320)
                     /locus_tag="ACGI57_003104"
     CDS             complement(5574..6320)
                     /locus_tag="ACGI57_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFG9718160.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
BASE COUNT         2092 a         1165 c          594 g         2573 t
ORIGIN      
        1 taaaggtaaa cactatatat taaaacttta tatcattata taatacaaat tttattgtat
       61 ttggtttgtt ttatctggtt catgatgttt ataataatat accgtttctt ctccaattac
      121 ttcagaacca agcttctccc atttttctcc ataggtagcc tctatttgtt cacattctga
      181 atctgttgat gctacaataa gaccataatt aggtctgaat atagctgttt cttcctttcc
      241 atttttataa cttaatttac cattatttaa ccttaacaag tttccattct tttgataatt
      301 aaaattaatt ccattaataa cttctggatt agatgaatca ataaatatat aattgttaaa
      361 gttatttttt aacattaaat tacctcctcc atgtaccgat ttaacgatat aatcataggc
      421 atctatattg catctcaatg ttaattttgg tatttgaagg gttttaaata cataaacttt
      481 atgtttatat tctaagccat catcccagtt atccttatta tcactaacta aatcaatatt
      541 acattctgta aaataatttc cataaaataa agaatatgct tcttctactc tatctttaat
      601 acttaaatat gtgtcataat ctgtattata ataatactga ccacaccttc ttataatacc
      661 agaaaaataa ctatctttca ttgaatcaaa tgaaaagcca caatacccac attggtcaat
      721 cgtaatatta ttaattttat aacttccata cccctcatac cccttaactt gactacttat
      781 aagaccaact ttctgtattt tttcaatcct tacattattt atactagttc cagttctaat
      841 cctcattcca ttttgacact tagatattct actattagaa ataattgtgt ctattcctgt
      901 atcaataccc agcccacaag atgaaactgt aatatcattt acaattgagt aatatggtat
      961 cctaattcct gtacccgaaa aaccacttat ataaatattt tcataatgac ctagaccttt
     1021 aatcatattc ttactgttat catacaggat tgctgataca ttattcatct caacattctt
     1081 ttcattctta ttaataatca tctcataatg atatttagga ttaccttttg tcggtggttc
     1141 tttatttacc tgtatctcac aactatcaga atagaaattt atatttttaa tacattgttt
     1201 cttccctgtc aatgtaataa tactcatatt ttctactcca cgagttataa attgtgtagt
     1261 acctgtagat gaattagaat caccttgcaa tacaatttct ttattaattt taacaccact
     1321 tgtaaatagg tatttacctt ttggaaaata taagacacaa aaatctgttt ctatttcatc
     1381 aataatatcc tgcaaagcag gacctatgtt gtacgaacca tcatttttaa tacctctttc
     1441 tactacatta atccatcttc catctttaat cccagttctt ccttccataa tattatatat
     1501 tcctttccct tttacttagt ttgaacctcc tatgactaaa gtcacagttt tactacttca
     1561 tagaaattta cataatcaaa ttaatttttt atattatttt tatattattc aatccatgct
     1621 attaccaaac catttatatt aaaactatag tcctaactct ccaataaaat acatctattc
     1681 aaatactacg tatttactta tttctctatc aatttataat agtgtacagt tattccaaac
     1741 tctatttttt caccaatttt ctcccatgtt cccccataat gagcagaaag aatactttta
     1801 tctttaataa ttgttgacat aatgtcacct acctctggtt tatgcatctt aatatcctta
     1861 tcattactat ttatatatat atttcctttt tttatcttga ctttatcccc ataatcttca
     1921 tcagatatac caattccatt gatactactt agttttccat cgtaaaatat aacagtttgt
     1981 cgcatattat tgtacgttaa atttcctttt tctgacttaa caatccagtc agtagcatct
     2041 atattgcatg ttaatagtac attcttggtt ttaagtgctt ctataagata caccttatgc
     2101 ttatcttctt tgaaatcttc ccaattatct tcattggtat ttgataaaac tatattactt
     2161 gactccaata aattaccata gaataaagaa tatgcttctt taactctatt atccaacatt
     2221 aaataattat catagcttac atcataatag tactgcccac accttgtgat atttcctgat
     2281 atctgaacat tggatataga atcaaaccaa actccacaat atccacattg gtcaattgtg
     2341 atatttgtaa taaaatttaa acctctacca atatttttaa ttccaacttt ttttatttct
     2401 tctaccctca taccatttat aaaagttcca gtagaaatta tcattccatc aacacagccc
     2461 caaaccttac tgttagcaat tgttgaattt tcccctgtac taattccaag tccggatgta
     2521 aaaattgtaa tatcatttgc tgtagaatta ttaggcatat caagtgcaat tccagaaaaa
     2581 ccactaaaag atagattttc ataatgacct ggttctccct gtttatcttc acatataaca
     2641 gcagatatat ttctataatt tataattaca ttatgatgaa atctaggttt tccatctgtt
     2701 ggaggttcat gattagatgt catatctcta tcacaactat ctgaataaaa gcaaatgttc
     2761 ttaatacagt gtcttacact ttttgctgtt atgattgctc tattttcact tttaggattt
     2821 ttctttcctt taaaagtaaa ctgtgttact ccagcagtat ttttaggaac attcgctaaa
     2881 atataagaat ctccacataa ggttaatcgt ttcttaatct taacaccact ttcaaataag
     2941 tattttcctt ttggaaaata tataatagaa tcttctggta cagcatccat aatctcttgc
     3001 aacttaaaac ttacatcttt tgtttcatca ttacaaattc cataatcaag tacattaatc
     3061 cagccactta tatctttaat tattatagac tcttccatta atcactacct cctagtttga
     3121 taatttaatc ttttatcttt ttataatagt acatggattc cccagctaat acttttacat
     3181 caatctgttc ccatattcca ccatgtaatt tatttatttc ttctgtagaa tctagtactg
     3241 aatatattac agtattcttt tttaatctag gaaccattaa atacttacct ttatcatcta
     3301 tatacaaagt gttctctatt attttgatta aatctttttc atctatatca gaaatttgaa
     3361 cacctcctac agaacaaatc tcaccattat aaaatttata agtattttca ccattttcta
     3421 acagcaagtt gccctttgca cattgtataa agttatctgc ttcagcataa catgttagca
     3481 ttacattgtt tgtttcattt gccttgatta catatatctt atgttcatta agtgaatcac
     3541 tttctaaggt atcaatattt ctattcatca atacaatatt gcaactttct aaaatatctc
     3601 cataaaaaat ggaataagct ttttcttttc tatcttccat attctcataa gcattataat
     3661 caacactttt ataatactga ccgcatctag taattctggc agttatctgg ctatagcaca
     3721 ttttttcaaa cataaatcca caatatccac attggtcgac cataagattc ataatcaggt
     3781 taaaaccaaa tcctttatta ttaataccta tttcttttat ttcttctatt ctcacattat
     3841 ttaagctaac accagtagta atctccattc catttttaca ttcgtaaatc ttgctactag
     3901 aaagcattga cttctctcca agcctcatac caagaccaca tgaactaaca attatatcat
     3961 tgccagttga atagtatgga atttcaatcc ctatccctga aaatccagaa ataaacaggt
     4021 gttcaaaatg agatagtcct tgtgtttctc catattctgt aatagcagat acatttttag
     4081 gcggtatttt ctctatatca tgactatcat aataaaaatt aatatttttg atacattgac
     4141 ttgttcctgt caatgcaata atactcatat tagaaactcc tcgagttata aaattggttg
     4201 ttcctatgat tgattgtttt ttatctaaat ttgatacttg attatctcct ccataataag
     4261 aatctccttg aagtgttatt tgttgactaa ttttaatacc tttctcaaat aaataattac
     4321 cttttggaaa atatataatg gaaccttcta gtgacttatc agagacaata tcttgtataa
     4381 tattacttac atcatatttc ccagtaatat ctattttact gtttaattct agtacattaa
     4441 tccaatccgg agttttagtt tcaatttcca tgattaattc ctccctttta aatctaaatt
     4501 cttttcactg aaaaaatttt ttgactataa ccgaagtgac ttttactctt tttgggaatt
     4561 ttgaaatgac aattttcatt ttacaacatc attatagtta tattataaat caattataat
     4621 ctcattatat tttcactatt accaagttat tcttaatttt ttaatacaaa ttttatataa
     4681 tgattaaaat agttaaactt tgtatcaata taatttaatt ccaataaaaa aacactcatt
     4741 tctgagtgtt ctatacaaat ttttattttg aaaaatctta tataatctta cattttaaaa
     4801 attttataat attcaattta ataccttctc tagtaaaatt atttttgatt tgcatatctt
     4861 aatgaccatg ggcaactcat catacacttc aatacacaaa aacactttga aacatcaata
     4921 atagactctc tttttcctgg caaagtccac tcatttccat gtatagcttt tgcagggcag
     4981 ttttctacac aaacattaca atccatacat tttgaatcaa tcaaaggata tctttcaaca
     5041 gaaattggag catctgtaag tactgtacac atgctaaaag cacatccgta ttcctctgtt
     5101 acaaacaaat tattttttcc cataaatcca atgccactaa tatttgctat tgtcttatgt
     5161 ggtaaaggac taatccctga ttgcatttct ggattaatat aggctttttc aaaatatcca
     5221 tgttctagat tgtttttctc cgattgagaa tacgctctat atcctttatt ttgaatatat
     5281 tctgaaatcc agtcagctaa ttcttctgct ttctcctctt tttctaaaaa ctcatcacta
     5341 tctgtaggta agttattgta tatatctgta ataaattttt ttgataaccc tatacaaaat
     5401 aaaattgctt tagaaaaacc ttgcgtttga tttattggaa attcagatat atcaacaaag
     5461 cgaacaatgt ccacaccttt attgtataaa atagatttta attcatcatt catatttgac
     5521 tcctccccct agataaaaat aacattacac attagtaaaa tttattattt taattatgtt
     5581 aaccaatatt ttgtacattc tttatttcta ctcataaaac catactcaat tagatatctt
     5641 ctaattgttg caaaatcatc atatatagat tctaaaatct gattaacttc cttttctgaa
     5701 tatttcttcc cacgttcaaa ttgttccaaa atcttagata aaattacgac ctttttcttt
     5761 tcttttgaag aaaatacttt taattttaaa ggattcatac tttcaaatga tgtatccaaa
     5821 atgtgttttc tctcttcttc agttactaca tatctttcat ctaccattcg agcatgttca
     5881 tggattggta ttatagtact acttttatta gatttatctt caaatacaga ttcatatagt
     5941 gctaaataga gcttagcctg ttttgctttt tctctgaaaa taaatttttg atggcgtata
     6001 gtagatgtgg atactccaac ctgttttgct atctccttat ctggtaaatc actaataaat
     6061 aattgaaata attgtttttg attttcagtc aatgtgttgt attttgaatc actattaagc
     6121 aattgctcta aataatcact atgttctaat tccatatgaa gttcaatagc ttttgaagct
     6181 tcaaagaatc tattacctat tttatatatt tctcctacct catatgtttt tgtacattta
     6241 atgcaactat aacaatttaa atcatcatta aatatatagc ctcgtttaat atcatcaata
     6301 gaaagatttt caattttcat gttactacct ccatttcaca gtttacattt ttcataaata
     6361 tattatatta aagtttatta atataatcaa catttttatt ttttgtttat caaatctaat
     6421 tatt
//
LOCUS       DAYAOX010000206         6409 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.207, whole genome shotgun sequence.
ACCESSION   DAYAOX010000206 DAYAOX010000000
VERSION     DAYAOX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.207"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     repeat_region   99..325
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=166..194
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(984..3077)
                     /gene="pdcA"
                     /locus_tag="ACGI57_003105"
     CDS             complement(984..3077)
                     /gene="pdcA"
                     /locus_tag="ACGI57_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdcA"
                     /protein_id="HFG9718161.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVKNPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SVQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
     gene            3721..4653
                     /locus_tag="ACGI57_003106"
     CDS             3721..4653
                     /locus_tag="ACGI57_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFG9718162.1"
                     /translation="MGFIGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKT
                     KIYSNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSK
                     KAGALIYSIHPIFAFSNKNTDIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRD
                     KDTSSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNG
                     FTKALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNIT
                     IENAIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            4628..5455
                     /gene="panB"
                     /locus_tag="ACGI57_003107"
     CDS             4628..5455
                     /gene="panB"
                     /locus_tag="ACGI57_003107"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFG9718163.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIIKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            5474..6322
                     /gene="panC"
                     /locus_tag="ACGI57_003108"
     CDS             5474..6322
                     /gene="panC"
                     /locus_tag="ACGI57_003108"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFG9718164.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSIFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         2418 a          890 c          781 g         2320 t
ORIGIN      
        1 cctttttgtt tttataatat agtgtaattt tattatattt ttgtatgcta atgtaactag
       61 ataatcataa gataacacat taaaagaaaa ttatgaaaac ttacattctg tatagttaat
      121 ataaaacata agtaatataa atttatgtaa agcaacaaaa gatagattta cattccatat
      181 agttaatata aaaccttaga gaaagcgaaa acgtaccatt aaaatcaaat atttacattc
      241 catatagtta atataaaacg ttgtaatcat agttccaatt agccacttta acatgcattt
      301 acattccata tagttaatat aaaacaccaa aataaactta gtattttcaa cacttacacg
      361 tatacaactc tcctaaattt gcagtgaacg agcaatagtg caattgataa cgcttatcat
      421 gcactctcaa cgccttatat ttcaactgtt acgccaaatt ttttcacaaa tatcgctcac
      481 tgcaaaattc ctataatttt attatatcat aaatatatta cttttgaata ttgataccaa
      541 tttataccat aataaaagca aaaaaacgta atctatctac attagattta aattctataa
      601 cttaaagttt tttacataat atttatgctt gtttaatcaa tctaatatat cattcttgta
      661 tattgctttt aatagtcttt tttcttaaat aaatatctat ctgaacaagc atagtttaat
      721 taaaaacttt tgtattccct taaaactgta tctacctctt attattttat ttatgatata
      781 ttatttttaa tctaaaaata gatatctatt tttattatgg ttatcccttt ttaaaatact
      841 ttaatcatat actattccca atttaacatc cattgaaatg ctattttctt atattgcaat
      901 ttataattga tatatcttca atataataat ttaatgtatt tttgatttta aagcttttca
      961 gtgatattat tgataaatta gaattaatta tctagcttta aaaggtcaaa gatttctgtt
     1021 gctgtctgcg gttttccaaa taaatatcct tgtatgtaat ctggctttat ttgtttaact
     1081 aaatcatact cttcttgtgt ttctacacct tctaaacata cttttattcc tacatcatga
     1141 caaattgcaa caataaaatg tataaatgta gcatcaaact tactctttaa tatatcttta
     1201 acaaatactc tatcaatttt cactatatct attggagaaa attttaaaac ttctagagac
     1261 gagtatccag taccaaaatc atccatagca atataaattc ctaaatcttg taaagcctta
     1321 aatttaaact gtaaaatact catattttgt actgtatgac tttctgtcaa ttctaaaaca
     1381 agattttgat aaggaaaatt ttcatcatct ataattttta ctatatcttc aataaatgtg
     1441 tcttctaata tttgaacagc tgaaacgttt acactcactt taaaaaaagg atagtaatca
     1501 atccatttcc taaaagtacg taatgccatt cgaagcaccc aagcccctac aatatttatc
     1561 atatcatttt cttctaatat tggaataaat tccaaaggag aaattgcttt gcatttgtca
     1621 ttagtccacc ttaaaagaac ttctacacct attattttat gactttctgt atctacttgt
     1681 ggctggaatc gaagactaaa ccccctaaaa tcatcattaa ttgatgcctt taaatcccgc
     1741 atcatttcta atgaatacat tttatttttt aatatttctt gggagaaaaa aacaagcctg
     1801 tttttaccat gctctttcgc atattgtaaa gagtaactag cacacttata aagttctttt
     1861 actgtatctc catgttttgg atatattaca caaccagcag aaattgttat atttaatcca
     1921 tattttctcc atagttgaag gtgtataatc atattctgta tttgattata tagagttaga
     1981 atttcatttt cttcaacatt atcaacaaga atgcccattt cgtccccatc aagtttataa
     2041 agttcagcat tatctggtaa aatagattgt acagactgtg ctagtgtttt tattatatta
     2101 tctccaaaat ccctatcata catctcatta acatttttaa aatcgtcaat atctaataat
     2161 actatgcata atttttcaat cataggattt gaaattttat cttcaaatac actcataaat
     2221 tcatgataat tcagtaactg tgtcaatgaa tcaatcttat tttgtttacc taattgcgtc
     2281 attattcctg caaaaatact tggctctcca aattcatcac gcattagttg acctctacat
     2341 cttaaccata tatattctcc actacgattt ttagccctaa attccactgt atgataatcc
     2401 atctggtttt tacctatttc agtgtttgac ttataaaaac gattccagtc ttctggatgt
     2461 acaatttttt tccagtaaac aagcggattt ttaacaattt ctcctggtaa gtcaaataat
     2521 tcaacttgtg atggtgaata tcgaaatacg cctgttttca tattgcaaat gtatatatat
     2581 tcatctgtac ctctaatcaa agcatcgtaa agcaatgtag aattataatc aaaattgtct
     2641 cttgaaaaat taaaaactcc tttttcttct ttattatttg atatttcatg aaattcatta
     2701 atcttaattg gtttatttaa gcttgttgag tttgcaatat ctattgattg atgcatatga
     2761 acaactgtgc catcttgcca tgtaattaaa ctatcataat tttcaaaaac acgatttaac
     2821 ttattacatt tttcatacca ctttattaat actccttttt tatcattctt taataattct
     2881 aaaactttac aaaaactaca agtagaattt ttttcaggat ataatacttg ccaacaaact
     2941 tttccttctg gatctaaaat attatattct tctttcattt ttttgttcat aaaaataatt
     3001 tcattagtat gaatatttgt aacatatata ttaatctgta gttgatttaa tataacttca
     3061 aaattatgtt tgttcatata cttatctcct ccatagttac tgctctttat aacaacattg
     3121 ttaaaattat tctttttaga agatttactt ataacttata catatacact aaatacctta
     3181 atttaattag aaagtttatt acttcatatt ttaaattata attgataaaa aaaaatacag
     3241 caatatagta ttaccaattt aaaaaaaatt aatattacat aatacatctc tctcttatat
     3301 taaattttaa cttggtacta atcttgacaa attgtccaca aaaataaatt atatatttta
     3361 ttattgtttt aaatttttat aaaaaatcag tattattgat actattgaag ttaaaaagca
     3421 ttaatttcta aaaacatcat aatttttcta atagacttaa tcacttttaa tatatatagt
     3481 aaaacaaaaa acttatattt tataaatatc ataatttttt tactgatatt ttattatata
     3541 aattttatag aattaattgt caatttttta tgaaaatggt aagatattta tggatggtat
     3601 cttgttataa gaactatact ttaaatgttt ttaattattt tgtcttatgt aactcatatc
     3661 ctcaggaatg agattagaaa ttaagatgtt gtatcacaaa gtggaggtga tactattaaa
     3721 ataggcttta ttggttctgg aaaagttgga acatctttgg caaattattt tttatcaaaa
     3781 ggttattgtg tttcaggctt ttatggaagg aatcaattat ctttattaga atcaacaaat
     3841 ttaacaaaaa caaagattta cagcaatttg aaagatataa tttatgaaaa cgatatatta
     3901 tttattacaa catcagatga ttctattgaa attatagaca aaaaactttc caaatttaat
     3961 ttaaaacata aatacatttg ccacacaagt ggctctttaa aatctagtat attgttctgt
     4021 tcaaaaaaag caggtgcatt aatttattct atccatccta tatttgcatt ttccaataaa
     4081 aatactgaca taaaaaaaat gaaagacatt tgtttttcca tagaaggtga taatgaacaa
     4141 gatgatttag ttattcaaaa tttcatagat gatttgggaa accaattttt tataagagat
     4201 aaggatactt catccactta tcatttagct aatgttttag tttcaaattt agtattatct
     4261 ttactagata taggagtaag ttactttatt aaattaggtt tatctgaaga ggaatcttta
     4321 aaagctatca gtcctttagt aaaaaaaaac atagaaaata tacttgataa tggctttact
     4381 aaagctctta ctggtcctgt ttctcgtgga gatataacac caataaggaa acatctatct
     4441 gtattggata aaaaagatga ggatatttac aaaatactat ccttaaacct actaaaaata
     4501 attgccttag gaaatgacaa ttcattaaaa ggtataaaaa atattactat agaaaatgca
     4561 atcgaaaatt taataagtaa atctgaaaaa tacaaagaaa tttacaaact tttaggagga
     4621 aaaaacgatg aaaaatacca tacagacttt taagaatgct aaatatgaag gaaaaaaact
     4681 atctatgctt actgcttatg actactcaat tgcaaaaata atggatgagt gtgatataaa
     4741 tggtatttta ataggagatt cattaggaat ggtcataaaa ggtgaagaaa acactctatc
     4801 tgttaccata gacgaaatta tatatcatac aaaagctgta aaaaatggcg taaaaaatgc
     4861 cttaatcgta agtgatatgc cttttctatc ttaccatgtt tctatagaag atgcagttaa
     4921 aaacgctggt agacttataa aagaaggagg agctcatgct gtaaagttag aaggtggctc
     4981 aaacgttata aagcaaatag aaagcattgt aaatgctcaa atacctgtaa tggggcattt
     5041 aggattaact cctcaatcag taaattcatt tggtgggttt aaggttcaag gaaataccag
     5101 tgaaacagca agacaactta ttgaagatgc taaattaata gaaaaagcag gtgcattctc
     5161 tattgtcctt gaaggagtgc ctacgaaaat agctgagatg gtaactaatt ctatttcaat
     5221 tcctacaata ggtatagggg caggaattaa ctgtgatgga caaattttag tttatcaaga
     5281 tatgttggga atgtttggag attttgttcc taagtttgta aagcagtatg caaatatagg
     5341 agatataata aaagattcaa ttaaaaatta catcctagaa gttaatacag gtgcatttcc
     5401 acaagaaaaa cacagctttt caataaatga atctgagtta gaaaaattat actaatatta
     5461 aggatggtac aaaatgcttg taaaagaaat taaattacta agaaatataa tcaaagattg
     5521 gagaaaacat ggctattcta taggccttgt tacaacaatg ggatttttac atgaaggaca
     5581 ccaaagtctt ataaaaaaag ctgtaaaaga aaatgataaa gtagtagtga gtatatttgt
     5641 aaatccaact caatttggac caaatgaaga ttttaatagc tatccaagag atatagataa
     5701 agattttaaa tattgtatgg atagtggagc aacagtagtc tttaacccat caccagaaga
     5761 aatgtattta aaaggtaatt gtactaccat taacgtttct ggacttacag attttctttg
     5821 tggagctaaa agacctgttc attttggagg tgtctgccta gttgtttcaa agtttctaaa
     5881 tatagtaaca cctgataaag cttattttgg agagaaggat gcccaacaat tagctgtaat
     5941 taaaagaatg gtcaaggatt taaacataga tacagaaata ataggctgtc ctataattcg
     6001 tgaaaatgat ggattagcaa aaagttcgag aaatacatat ctctctgagg aagaacgaaa
     6061 atctgctcta atattaaata agagcttaag tttagcaaaa gaagaattag taaaaggcaa
     6121 tctaaatcca gagaatataa aagaattaat aacagctaaa ataaattctg aacacttagc
     6181 aaaaatagac tatgttgaaa ttgtagatag tgagacatta caacctgtta agcaaataga
     6241 acattctata cttgtggcaa ttgctgtgtt tataggaaaa accagactaa ttgataattt
     6301 tacatttgag ttaaatatat agtctataat tttatataat atatctaaaa aataatagaa
     6361 tacctaaaaa gtaataaatt acctaaaaaa taataaattg cttagaaaa
//
LOCUS       DAYAOX010000207         6388 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.208, whole genome shotgun sequence.
ACCESSION   DAYAOX010000207 DAYAOX010000000
VERSION     DAYAOX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.208"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            28..1011
                     /locus_tag="ACGI57_003109"
     CDS             28..1011
                     /locus_tag="ACGI57_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFG9718165.1"
                     /translation="MTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIKVIKH
                     NNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGIGLDE
                     LILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDFGITK
                     ANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNSEEKM
                     LQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKAYIEV
                     EDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            1257..2324
                     /locus_tag="ACGI57_003110"
     CDS             1257..2324
                     /locus_tag="ACGI57_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFG9718166.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFAEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            2858..4768
                     /locus_tag="ACGI57_003111"
     CDS             2858..4768
                     /locus_tag="ACGI57_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFG9718167.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNVGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(4831..5454)
                     /gene="ytaF"
                     /locus_tag="ACGI57_003112"
     CDS             complement(4831..5454)
                     /gene="ytaF"
                     /locus_tag="ACGI57_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HFG9718168.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            5642..6286
                     /locus_tag="ACGI57_003113"
     CDS             5642..6286
                     /locus_tag="ACGI57_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9718169.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         2728 a          609 c         1009 g         2042 t
ORIGIN      
        1 atttagttat ataaaatgta tgaggaggtg acatggttga attttatagg caatagatat
       61 gaggtagtaa attctgacga cgttttagaa attaacaaaa tctataaagc tagagatgtt
      121 ttttacagga aaaatgtgtt aataaaagtt ataaagcaca ataattatat ctgtgaagat
      181 tttgtttcaa atttaattga tgaaagtact gctttagatg agataaactc tccttatata
      241 ctaaaaataa tagatgttgg gattcactgt acagaagaaa caacattata ttatgttgta
      301 agtgaagact ttaatggtat cggactagat gaattaattc taggtaatta tcttcattta
      361 gaagcaattg taaacataat gatacaagtt ttaaaagctt tagaaatgat acataggaat
      421 cattcatatc atggtagttt aaaatcgagt agtataatag tagatacaga atacaatata
      481 aaaatttgtg attttggaat tacaaaagca aataatgggg ttaatacaag aagctatgga
      541 aacaggagat atttatgccc acatcaacta tgtataaatt atactgataa agaaagtgat
      601 ttttttgcaa caggtcttat tctatttgaa tctatattta aaaaattgcc ttttggagaa
      661 tcaaattcag aagaaaaaat gctacaatct attgataagg gattagattg gaatagtata
      721 aaagcaatta atggaaatac tgaacttata aatgttataa aaaggcttct tggaagaaat
      781 aataagtata ctagagccaa tgatattatt attgatttaa gtaaagtaat gtatgaaaaa
      841 gcatatatag aagttgaaga tgagaaagaa gaagagcaga aacaagacaa agttatacaa
      901 gttaaaaaag aatataaaag taaaatgaga aaaaaattac ataaaaaact tgttgttgcc
      961 ggtctagcta tagtcatgat ttcaatgatt attattagtt ccataatata aaaattgtat
     1021 attaagtatt ttaaaatcaa tgtctataaa ggatatacat agaaatttta gtgtagtctt
     1081 aaattatgaa aggaagtggt tattatgtta acaaaaaata atgaggtatt aaacgtctag
     1141 gagtatttta acaaatagcc acctgctttt acagtttaga ataaaaaagc tgtggctaaa
     1201 ggtcacagct ttttgctact ttttaaaggc aggagaatat taaaaaggag caaattatga
     1261 gcaatataaa aataatagca agtaataaat gttggattga agatatagca aggaaacaac
     1321 ttgaagatat ttcaaagtta aatggaattt taagagtagt aggattacct gacttacatg
     1381 caggtaaaat acctgtaggt ttagcagttg agactaaaaa tattatttat ccacatataa
     1441 ttggaaatga tataggatgc ggaatgactt tatttaaaac tggggttcta aaaaagaaat
     1501 ttaaaaaaga caaatggatt aagtcactta gtaagattaa agatttgtct gatatagaaa
     1561 taaaaaacac atataaagaa gaatgtccaa ttttaaattt aggaactata gggagtggaa
     1621 atcattttgc agaaattcaa tgtataagtg aaatttataa taaagaacaa tttgaacaat
     1681 taaaattttc atctgatgat attatgatgt tagttcactg tgggtcaaga aattatggag
     1741 aagatatttt aaaaaaattt tatgataaag atggacttga agttgaaagt gagaaagcta
     1801 tagattatat taaaaaacat gataatgctt taatgtgggc agagcgtaat cgtgaagcta
     1861 tcagtaaaaa aattatgcag tcaataggta catctggaga tgttgaaaca atattctcta
     1921 taaatcataa ttttatagag aaaagggaag ataaatttat acatagaaaa ggtgctgttt
     1981 caagtgaaag aggagctgtg attataccag gttcaagagg cagtctatca tatatagtta
     2041 tgcctactga aaatacagag atatctttat attctctatc acatggagca ggtagaaaat
     2101 ggtctcgttc agtttgtaaa tctcgcctta aaagtaagta tagtaaagat actataaaac
     2161 aaactaaatt taaaagtcag attatatgta atgatgtaaa tttattattt caagaagctc
     2221 cagaggcata taagaatata gagcagatta ttgaaagttt gatagaatat gaacttattc
     2281 aagtagtggc aactatgaag ccattgatta cttataaagg ataattttta aagttaaaaa
     2341 agattaaaat tatattataa atagctaatt ttatggaaca tttaaaagtg ttttattatt
     2401 agctattttt ttattttgta gagacgataa tttatagagc taaattcttt gatatataat
     2461 gattacaaag ttagactgta ttggaaactg cacatacatg aattgatgac aatataagtg
     2521 attcccaaat ataaatgaaa gtataagctt atttttacta taaaaatttt cactgtacaa
     2581 aaacattaat gacattattt ttattgacat aaatatataa tttatggaat aatattagaa
     2641 gcactgatat aagtgtttaa ataagaatag aaaaggagaa gtgttttatg aaaaaaaata
     2701 ctaacattaa tgagtccgga gttaaacttc ttaataaata aggagtttga tatataataa
     2761 ctccttattt attaagttgt tcgcaaaata actattatca aagtaaatga tagggtgatt
     2821 taaaagacga tgacttataa ataaaggaga aaaaataatg aatagtaata catttgaaag
     2881 attacaacta aatgaagtta aagacttaat aaaaatacat tgtgtaagtt cgctaggaaa
     2941 aaatttaata gataaattaa ctcctagtgg gaatattgga gtagtaagaa ggaaattagc
     3001 agaaaataaa gaagctagaa agataattga aaatagtaat cacataccac ttgaaggatt
     3061 gtttaatgta ggttctacaa tagataaaat tgaaaaagga atgataatag aacctgttga
     3121 acttgtaaat attgaagatt ttttaagagg ttgtaggaag atgaaagctt ttatgttaga
     3181 aaaagaattt tattcaccta cattaagttc ttatgcctta aatataacag aatgtaaaag
     3241 tatagaagat gagataaatt actgtataaa atctaataaa gttgattcta atgctagtaa
     3301 agaattaaaa aagataagac gaaatataga aattacagaa ggaaagataa aagatagatt
     3361 aaataaattt ataacatcaa ctgtaaataa aaagtatata caagagttta taataagtaa
     3421 aagaaatgat agatatgtaa taccaattaa atcttcttat aaaaatgaag ttaatggtac
     3481 tatattagat acatcatcaa aaggaaatac tgtatttatt gaacctatta gtgtttcaaa
     3541 tttaagtaca gagttaacta tgctaaaagc tgatgaaact attgaagaat acaaaatatt
     3601 atcttattta acagaactta tatttgagaa gataagtcag ataaaattaa atatagagat
     3661 actttcagaa tacgatatgg tatttgcaaa agctaagtat agtcaaaaaa taaaaggaat
     3721 tactccaaaa ataaataata atggctatat aaaaataatt aaaggtaaac atccacttct
     3781 aacaggagat gcagtacctt tagattttga aataggcaaa aattatagaa gtcttataat
     3841 aacaggtcct aatgcaggag gaaaaactgt aacccttaag actgttggtt tactaacttt
     3901 aatggttcaa tgtggatttg atatttctgc aaaagaaggt agtgaattta gtgtatttga
     3961 aaaagttttt gtagatatag gggataatca aagtattgaa aatgccttaa gtactttttc
     4021 atcacatata aaaaatattg cagagattat gagtttatca aataattcta ctcttgttct
     4081 gtttgatgaa ataggaagtg gaactgaacc taatgaagga gctggtttag caatatcatt
     4141 attagaagag ttttacaaaa tgggatgtat aactatagca tcaactcatt atggagagat
     4201 aaaaaagttt gctacattgc atccagaatt tgaaaatgct ggtatgatgt ttgataaaga
     4261 aacgttagaa ccattgtata agttaactat tggaaaatct gaagatagta atgctctttt
     4321 tatttctaaa aaaatgggaa taaaaaatag agttttagat agagctaaag aatatgtaat
     4381 tgatagaaac tacaatttag atttagttaa aagaagtaag ctacaatcta atattgaaga
     4441 taaaaatagt tctgaagtca ttttaaacta tacagattat aatgtagggg ataaggtaaa
     4501 attactagat gaagatgatt ttggaattgt atataaacct atggacaagt ttaataatgt
     4561 agaagtattg tttaatgata attttatttc agtaaatata aaaagattac aacttagcat
     4621 aaaagcatca gaattgtatc cagaagggta tgatttagat gtattattta ctagttttaa
     4681 agaaagaaaa ttagaaaaag atattcaaag aggttcaaaa aaagctctta aaaagattca
     4741 gaaagaaatt agagagaata gaaaataaat ataaaaagca caaaagaaag caatctaaag
     4801 agaaaagttt atctttagat tgctttctta ttaacatatc ttcataaatc caatgactaa
     4861 caagattaaa ccacttaacc aagataagtt taaatttacg tttttaacaa acatatttcc
     4921 tagtaaacaa ccaagtaata ctgcaattat atttgatatt aaagaaaata gaataacttg
     4981 tatataattc acacctacaa ggccactacc aaaaccgata gctaatccat ctaaacttaa
     5041 agcaagtgca agtgtaaatg cttcagcaga tttaagcact ttagatttac ttgtatctaa
     5101 gacatcaggt tcgatactta cattaaaatt taatttaagg tctaacatat taaaattata
     5161 attctttgag tgtatagatt ttttcttaaa ataagattta aaaaaacttt caattagtct
     5221 agcacttcct aataaaaata aaataaagaa acatattaac attgttatat tttgaggtaa
     5281 aaagtctcta actattgtac ctgcaaaaag cgatattgct aaaatagctg aacaaactac
     5341 gctgataatt atatttgaat ataagggaac tttaacttta tttacgccat atccaaagct
     5401 tgtcacaaag gcatctatac aaagagaagt aactaataaa attgattcaa tcacagtaga
     5461 ttaacatcct ttctcttaaa ttttgtactt atattagtta tagtattgta aaaatggata
     5521 aattgttaca gataatccaa taaattgttg ataaaattcc aacatcatac aacacctaaa
     5581 taacaaaaga atcatatcat ataataatat aaaaccaatc ataataaagc gggtgaaaat
     5641 aatggaacaa aataataagg ttgggaattt cggtgatttg ttgaagattt tattagatga
     5701 aaaagaatta tcaatgggag aactaagtaa aaaaagtgga attgataaat ctactatatc
     5761 tcgtatagct aataataaac aaaagccaaa tataaatcat ttagaaaaga tggcaataca
     5821 tttaaatatt aacctagaag aactgttgaa agcttcagga tatgaattta gaaacagcaa
     5881 taatcaaata tttaatgcag atagtgattt tagtaacttt gatgatattc ttggatttgc
     5941 aaatttaatc aatgataaga actttaatgg aaatattgaa aaagagttaa gtaagtgtaa
     6001 gttatatgtc caaacagatg aaggtaaaaa attacttttt gataatttta ataaaaaaat
     6061 agattctatt gaaaaacaag gtcaatttac agatagatta agacaaatgt atgctgattt
     6121 ttgtacagat ggacttacaa taaagaaata tttattgatt ggaagtatgc tactttattt
     6181 tgttatatca acagatgtga ttccagactt cgtattccca ataggattta tggatgattt
     6241 agtagcatta aatatagtta caaaattact taaaaatgat aagtaagttt tagaatcata
     6301 tttaatatat aaataaatca aagtatataa aatttattat tttttatatg aaacaaatta
     6361 aaagcacaat gaagtagatt aaaaattt
//
LOCUS       DAYAOX010000208         6388 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.209, whole genome shotgun sequence.
ACCESSION   DAYAOX010000208 DAYAOX010000000
VERSION     DAYAOX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.209"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            191..1384
                     /locus_tag="ACGI57_003114"
     CDS             191..1384
                     /locus_tag="ACGI57_003114"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFG9718170.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1590..2708
                     /locus_tag="ACGI57_003115"
     CDS             1590..2708
                     /locus_tag="ACGI57_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HFG9718171.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2839..3084
                     /locus_tag="ACGI57_003116"
     CDS             2839..3084
                     /locus_tag="ACGI57_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFG9718172.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3071..3817
                     /locus_tag="ACGI57_003117"
     CDS             3071..3817
                     /locus_tag="ACGI57_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9718173.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4102..4977
                     /gene="hslO"
                     /locus_tag="ACGI57_003118"
     CDS             4102..4977
                     /gene="hslO"
                     /locus_tag="ACGI57_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFG9718174.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5368..6168
                     /locus_tag="ACGI57_003119"
     CDS             5368..6168
                     /locus_tag="ACGI57_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFG9718175.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
BASE COUNT         2550 a          609 c         1257 g         1972 t
ORIGIN      
        1 ttagaaaaat tttaaataaa tgttttattc tcaaaatatg catattaatt taataaaata
       61 taccaaaatg gaaataaaaa ataaatttta tgagaattta ataactaata attaatatat
      121 ttaggttata ataaatatgt gcgtaaaatc taacaataag caataatacc gtaaatttag
      181 gaggaataat atggatactt taaaggaact ttttaaaata ggaagtggac cttcaagttc
      241 tcatactatg ggaccacaaa gagcagcaga gagatttaag aatgaaaacc cagatgcaga
      301 aagttttaga gcaatattat atggtagttt ggcagctaca gggaaaggtc acttaactga
      361 ctatataatt gaaaaaacta tagcacctaa aaaagtcgaa attgtttggg aagaagatat
      421 tataaaagat tttcacccaa atgggatgaa gtttgaagct ttagataaag ataaaaatgt
      481 aacagctgaa tggactgtat attctgtagg tggagggact atagcagaag aaggtcaaag
      541 aaacagtaaa agcaatagta tatatcactt agatactatg gatgagatag taaaatggtg
      601 caaagaaaat aataagactt tggtagattt tgtgttggaa tgtgaaccaa aagacataaa
      661 agattatata aaaactataa aagatgctat gagaaaatct attgatgatg gactaagtac
      721 agatgaaata ataccaggca agttattatt aaaaagaaga gcaagtacat tttataatgc
      781 atataaaaaa gataagagct tctcaacact tgtatatgca tatgcactag cagcttcaga
      841 gcaaaacgct tctggtaata taatagttac agcaccaact tgtggttctg ctggagtaat
      901 accaggaata ttctttgcaa tgcaggattt ctataattat gatgacgaaa aaataattga
      961 agcactactt gtagctggaa taataggtaa tataataaaa actaacgctt caatatctgg
     1021 agcagaggta gggtgtcaag gtgaagttgg agccgcttgt tctatggcag cagcagcagt
     1081 agcatactta aaaggtggaa ctatagacca tattgaatat gcggcagaaa tagcacttga
     1141 acatcattta ggaatgactt gtgaccctgt atatggatat gttcaaattc catgtataga
     1201 aagaaatgca atggcagctc aaagagctta tgatgcagct aattatgcat tattaactga
     1261 tggctcacat tctgtctcct tagaccaagt tgttgaaaca atgaaagaaa ctggtataga
     1321 tatgatggat aagtacaaag aaacagctaa gggtggactt gctaaacatt tcttctcttg
     1381 ctaaaaataa aattttccaa taataattcc tctgttttga tttatgaaac aggggaatta
     1441 ttattaatgg tgaattattt aataatgcaa aatattttta tattcaattt agctttttat
     1501 ttttaattag tataacatgc tatataatat tttaattata aagaaatata gaaatatttt
     1561 ttaataaatt agaaattgga ggagattaca tgagagaggc atcatattta caagaacaac
     1621 tttataaaca taggaagtca ttaaatccta ttgcagagat tgggagaaaa gaatataaga
     1681 cttcaaaata tataagagat tatcttgata aattaggaat tgagtatgaa gtttatttag
     1741 aaacaggtat agtaggtgta atagaaggta aaagacctaa aaaagatatt gcttttagag
     1801 ctgacataga tggtcttcat agtgaagaag gggtaaaaca tttatgtggt catgatggac
     1861 atgctactat attacttgga ttaattgagt atttaaatga taataaagaa gacttaaatg
     1921 ataatatagt atttatattc caaccagcag aagaaggtcc tgggggagca aaagctttaa
     1981 tagatgaagg agtgcttaga aaatacaata ttgatgaaat atatgggctt catatatatc
     2041 cagagttacc agaaggttat gtaggtacaa gagagggcta ttttatggct caaataggag
     2101 atattgacat agaaatagtg tctaaaagtg gacatggggc tatgccacaa aatggtattg
     2161 atggtatagt gatagcttca aactttgtaa gctcacttca aactatagtg tctagaaata
     2221 taagcccaat agacaatgca gtgttgacta ttggtagaat tgaaggggga gctagaagaa
     2281 atataatagc tgaaaatata aagatggaag gtactatgag atgtttcagc ccagaagtgt
     2341 atgagaaaat gaagcttaga gttagagaac ttgctagagg gtttgaattg gcttataact
     2401 gtaaggttaa tataaatatt atagatgatt atattgctgt taaaaatgac aaggatttgt
     2461 atcaagagtt tgtagaggct attggaagtg atactgttgt agagttagag ccacttatga
     2521 tatcagaaga tttttcttat tatcaaaaag aagttccagg attatttttt atgttgggtt
     2581 caagaaacga agaaaaagga tttgtaaatg gtcttcataa tataaacttt aattttgatg
     2641 aaaagatatg tgtaaatgca ttgaatgttt attcaaaatt attaaaatac aaagaagcaa
     2701 tagattagga agataagtta taaagattaa aaagcataaa tacctaaatt taaaaaaagt
     2761 gtattaataa atacccctta catatttgta taaatttatt ttaattgaca aaaatacaaa
     2821 taagaagggg tgattttttt gaaagaaaaa gtacaaagag aaaagaagat aactaatggc
     2881 aagaatacta aaatattgac aaatgatgat ataatgaaat atgagatagc ctcagaacta
     2941 ggtcttatgg ataaagttgg tgaacgtgga tgggcaggac ttacagctaa ggaagcagga
     3001 agaattggag gtatgcttac atcacgcaaa aagaggctta aaaagattgt ggaggagcag
     3061 aataaaaagg atgagtcaat atagtgattt tgcgtttgta tatgatgaac ttatgaatga
     3121 agttgattat aatggttggg ttaaatatat agaagatata atagatagtg aaggtgtaaa
     3181 agtaaaaaat atattggaat tggcatgtgg cacgggtaat cttacaattc cattgactaa
     3241 aaaaaattac gatatagctg gtatagatat atctgatgag atgcttagtg tagcaagaga
     3301 aaaggcagaa aaagaaggag tagaattagt tttacttcaa caagatatat cagagcttga
     3361 ttttgaaata tctgatttgg actgtgtact ttgtgcttgt gatggattta attatataac
     3421 ttatgatgat gatttggaaa atgtattttc aaagacatat gaattattaa agaaagatgg
     3481 aatattcata tttgatataa gttcttttta taaattagcc aacatattgg gaaataacat
     3541 gtatggagaa aatagagaag atatagcgta tatgtggcaa aattactttg atgaagaaga
     3601 gaatctaatt gaaatggaac ttacattctt tattaaagat gaatgtggaa agtttgaaag
     3661 atttgaagag atacaccaac aaagagcata tactgaagat gagatagttg atatgcttga
     3721 aaaatctgga tttaaggata taaaatatta tggggatttt acatttgaca aacctagtga
     3781 agaaagtcaa agaatcttct ttgtatgtaa aaaataagat attcttgact atataagata
     3841 attattaaaa ataaccatga tataaaaata tataaaatgc agttttatag attaaaagta
     3901 aagtgatgat attttatgat ttttattttc taataattat aaagttttat agataaaaat
     3961 taaaatataa taactattgc ttagtaaaat taaaataaaa tgggttttgc ccaaggttaa
     4021 gatagataag tatttataga ttaaaattaa ggtagtaata taataaaaat aagacaatta
     4081 acgggacatg gaggaatatt aatgagagat tatgtactaa gagcgacatc aggaaatggt
     4141 caagttagag catttgtcgc aactactaga aatacagtag aagaagctag aagattacat
     4201 gaaactacaa aagtagcaac agcagcactt ggaagaacat taacagccac atctataatg
     4261 ggattaatga tgaaaaatga tagtgataaa ttgactgtaa taataaaagg tggaggtcca
     4321 attggaacta ttatcgccac atcagactct aagggaatgg taaaaggata tgtaggaaat
     4381 ccacaagtag aggttgaaga ctatccaaat ggaaagttaa atgtagcagc agcagtaggt
     4441 acagaaggtg ttgtaaaagt tataaaagac ttaggtctta gagaaccata taatgggact
     4501 tatccattgg taagtggaga aattgcagaa gactttactt attattttgc tgtatcagag
     4561 cagactccat cagtagtagc tttaggtgtt cttacaaaag aagatgaagt agagtttgct
     4621 ggaggattta tagttcaatt aatgccagat gcagaagaag agactattgc taagttagag
     4681 gaaaatgtag ctaaattacc ttctataact aacatgttaa aagaaggaaa atcaccagag
     4741 gatatattaa atattgtttt ggatggtttg gaacctaaaa tacttgatac ctgtgaagtt
     4801 gggtttatgt gtgaatgttc aaaagaaaga gtaaaaactg ctttggttgc aattggtaaa
     4861 aaatcattgg ctcaaataat agaagaagat aaaaaagcag aagttggttg ccagttctgt
     4921 aataagaagt atatgtatag tgaagaagag ttgcttgaaa ttttaaagga aatgtagact
     4981 ataaaaatag ttgtattgat ataagtaatg ttatagtaat atagtaaagt cgtattgata
     5041 tagtaattaa agtataattt attaataata gacatattta tattaaaaaa taagtttaat
     5101 aaatttttaa gcttaataaa ttttaagaat taagaagctt tctggctgta atgtttgcaa
     5161 tgttgcagtt agaaggcttt tttattttat tatagttcgt gtcaaagatt gtatgaaaca
     5221 ataaaatatt gtaaaataat ttgtatattt agacctgatt gacacaaagg tttataagat
     5281 atttgagttg attatacgaa attctatgaa gtagtaaccc tgtgacttta atcatgggag
     5341 gttcagaaga aggagaaaat tttaaatatg gaaagtaaaa cgaagtacat aggaataagt
     5401 atattaatat tattgatgat atttttaatt gcaaagaatt ttaacggaaa acttgaaata
     5461 cctattcctg agagtaatag tagaaaattt aaatcagcag cagtgtttta tcctcaacat
     5521 caagatgatg aggttttatg gggaggaagt gccattgtag atgctataaa acagtgtggt
     5581 gttgataatg tatatgtggt attagtatca gatggttctg gagttaacgt tttcaaagct
     5641 aacacaaaat ttagaaattt aactaggaag caaaaggaag aacttagaaa taacgagttt
     5701 aaatcagctt tgagagagct tggagtgaaa cctcaaaatg tgattatact tgcagatata
     5761 gataaaaagg aaggcacaca ttatgaatta atggaaaaga ctatattgga ttttgaacat
     5821 aagtttaaag gcaatgtgac ccatatagcc catcactata agtatgatga ccatataatg
     5881 catagaaaga atggggaagt tttaaagaaa cttaaacgtg aaggtaagat aaaagatgac
     5941 ttgtattttg ttaagccaaa atatgtaaag tatataccaa gaaatgaaag agctatctat
     6001 aaagcaatgg attttgatga ctacaataag gttaagagtg cttgttatga atataaggtt
     6061 gtaaattcaa agcgtaaaat gtatggtata ggatatattt cggcacatag ttattttgaa
     6121 catcttctaa atgaccctga acttacgtct gtattaagtg aagattaatt tcttataagt
     6181 agtaagttat gtttgataag ttgattggaa ttttctatag ttttgaaggt attggaaatt
     6241 tagttttatt taaagattta aagatagagg tttttagtgt ttaaaatttt aaagttaagt
     6301 tgaataattg tatagaatta tttgtgaaag aagaaacagt gtaaaacata gtatcttatt
     6361 tatggtttta aaatcatagg caagatac
//
LOCUS       DAYAOX010000209         6385 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.210, whole genome shotgun sequence.
ACCESSION   DAYAOX010000209 DAYAOX010000000
VERSION     DAYAOX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.210"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            431..589
                     /locus_tag="ACGI57_003120"
     CDS             431..589
                     /locus_tag="ACGI57_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718176.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(876..1580)
                     /locus_tag="ACGI57_003121"
     CDS             complement(876..1580)
                     /locus_tag="ACGI57_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9718177.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1571..3178)
                     /locus_tag="ACGI57_003122"
     CDS             complement(1571..3178)
                     /locus_tag="ACGI57_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HFG9718178.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3432..4610)
                     /locus_tag="ACGI57_003123"
     CDS             complement(3432..4610)
                     /locus_tag="ACGI57_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718179.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4836..5330)
                     /locus_tag="ACGI57_003124"
     CDS             complement(4836..5330)
                     /locus_tag="ACGI57_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFG9718180.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNIGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5331..6092)
                     /locus_tag="ACGI57_003125"
     CDS             complement(5331..6092)
                     /locus_tag="ACGI57_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFG9718181.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
BASE COUNT         2153 a         1174 c          689 g         2369 t
ORIGIN      
        1 cattcattta aaaatataaa actattatat attaaaattt cttcaataat aaattatcta
       61 gattgttact ttgatatatt atataatatc acaaaggctg atataaaaag acaaaaaggg
      121 caattttttt atatcaaaaa aaaatgttaa attgatatta tccatatata aattgatatt
      181 atccatatag atgctatatt taacaattct acttacttcc cttacactta ttagcatata
      241 gttattaaga gtttgttgca aaacaacact tagcacactt taaaaagtgt ggtctaataa
      301 gtaatcaacg aagaagacat aataaagcat aaaatatgat atacttttcc acgccatcaa
      361 aactgaggat tttattatta atatatgcta aaatatgctt aataaattaa attttaatgg
      421 gagaatagtt atgaaaatat ttagtttctt gttgtttgtg ataggaatat gtttatgtat
      481 tgctggattt ataggacatt acccagtatt tgttggtaca acattgagtt ttcttgcaat
      541 tgcaatgata gctgtaagac aagtcaaaac taagaaaaaa atgaaatagg caaatcctaa
      601 ctttacaagt taatattaca caagtaatta gtctaaaatt ggatactttt tctattacag
      661 aaatacatca tccactttgt attttcttaa aaaaataaaa tagctatgta aattcaagat
      721 tagatagatt aactctctat ccatttcatt gtaaaatata catagctatt tgtattcgat
      781 ttaaaatact tagattttct tacataacaa ataatttcta tctgattatt actcatattc
      841 atttagtaac cactcagcaa ctaatcattt agtatctatt catccgccat acggtaacct
      901 accccaacct cagtcaaaat atattctggc tgagcaggat tcttttcaat tttccttctt
      961 atacttgcca taaaaactct aagcgactga ttttcacttt catagtaatt tccccaaact
     1021 ttatctatta taaacttatg ggttaagact cttcctgaat acttagacat caactcaata
     1081 atcttatatt cgattggagt taaatgtatt tcttcaccat taattgacac aatatgattt
     1141 tcataatcta tctttaggtt tttaacttca aaagactttg gagcatcatt atttatatta
     1201 tcctgttgag ctttatttct aagagccaca cgtactctag ccaaaagctc tgttacacta
     1261 aaaggtttag ttaaataatc gtctgctcct ccatctaaag cttccacctt atccctctca
     1321 tgctctctcg ctgaaacaac aattatttta ttagtctttg cacatgccct aactcgtcct
     1381 attacctcaa tcccatcaat atctggtagt cctaaatcca aaattatcaa atctgggtca
     1441 agagaatgta taagtgatat agctgttcct ccattatctg tctctacaca cctataattt
     1501 tgagtttcaa gagatacttt tataaattta caaattggct tatcatcttc tacaattaga
     1561 actaatggct ttatttccac ttgtctttcc cctcctcatc atacataggt atgctaaatc
     1621 taaatgtagc tcctccagaa ggattattaa aggcgacaat ttctccacca tgagcttcta
     1681 ctatagattt acatatctcc aatccaagac caatacctct tctttcaaga tttctagact
     1741 tattcttagt ataaaaccta ttaaaaatat tcccgatatc ttcatttggt attccttttc
     1801 cattatctgc aacttcaaat agtacttgat ttgactcttt ttttacagtt agttctatct
     1861 tagaattttt aggtgtatgc cttatagcat tatcaattag atttactaac acttgttcta
     1921 ttaataatac atccacatga acgattatta tctcatctgg aatatttgtt tttatgtctc
     1981 tactattggc aaactttttg actcttaata tagattcagc tattatttca tcaacaactt
     2041 ctggtcgttt ttcaatatcc aattttcctt catctattct ggtcatacta agtatattct
     2101 caacagaatg agttagccaa ctcgtatctt catatatatt tttcaataat tctactctag
     2161 tttctttatc tatcacatca tcattttcaa gtattgttga agatgaacct aatatacttg
     2221 taagtggagt tcttaaatca tgagaaattg acctaagcaa gtttcctctt aatctctctc
     2281 tctctgcctc aagatttgct tttttatttt tctcaaataa attttctcta tcaattgcca
     2341 acgcaacctg tgttgaaact gcttctaata gtatcttttc attttctgta ataatatcta
     2401 tcttaaaaca cgcaacacct ataacaccaa gaacatttga ttcttgacct tttataggga
     2461 aataatatgc actgctgttt ttacaatatt ttgtcccaat tccaactggg ttacctgtcc
     2521 taaaagcttc ttttatagct tctttttcta atactgaatg gaatatattc tcacttccat
     2581 cattgttaca aatataaata cttggcttac ctagattatt attctcatct gcaatagtca
     2641 ctattacagt tctatttaat atatttacta aattagaccc acaaaattca ataatttgat
     2701 ttttattttt tgctttaagc aacgttttgt tattctcata gagcaatctt atcatttttt
     2761 ctctcttatg agaaagtctc acttctcttt ttatcctaga agttaaagta cttgttataa
     2821 ttgctgccat tagcataata caaaaggtca taggataatc ttttctataa gctaaaaaag
     2881 tatataatgg atgcgtaaag aaaaaattaa atgatagcac tcctattact gaagaaagta
     2941 ttccaaaaaa atatccatag gttatacttg tagcaaatag tacacctaat atgtatacta
     3001 gtataatatt agattcatta aaacctacct tttcaaaaac tactgatatt ccagtagcaa
     3061 ttgtcataac caatatagtt tttatgaagt ccttaataat tatcttttta ctgagatagc
     3121 catcttcatc ataatcatac tcttttaagt cttcatgttg tagtcgttcc tttttcattt
     3181 actacaattc ctccttattg ttactgttta aattggctct attttatata atacaatttt
     3241 atcataataa attcaatcaa tacatttaat taatgtactt aaattactaa aaggtgctgt
     3301 cttaaaatga aaataaatct ctatttcaaa acagcaccta tatatttaaa ttactaattt
     3361 agtttttaca tacttaaatc aatagtttta ttcaatttca taaattataa tacttcaatt
     3421 ataaaacatt tctatccaac aagcataaat ataaagtggg cacatatacc tgcacataaa
     3481 ccacctatag tatgcgaaag tatcgcttta ccagccaatg gtcttgcatc taaagcatcc
     3541 atcataccta tgtgtgtact taaatatcca ctccaacaca tacccattgc tgtgaatact
     3601 gctatatcat taggagttat agcgcctgat tttataaact ctggtactaa agatattgct
     3661 gctccaacag ctcctaatgc agttacaggg aatgctattg cctctggtga tgtgaatcca
     3721 aataatggtt ctattatgaa gctaatctta tcaccaatag caggtaataa ttttatacct
     3781 tcatatgcag ctcctgtgta aacagcttga cctgtagcag ggtctgtaga tggtccaaaa
     3841 gttaataaca taactaatgt acaaactact aaaactcctg gtattatagc catacccatt
     3901 tcaacaccta acttaccacc ttcaagtata gcatccaatg ttctttggaa tacattacca
     3961 tctcttattt ctctaaactc ttcaagtttt gcacctttat catttatttg cttttcagta
     4021 tcatttttag ggtcataatt ataatacttt ttagtaaaag tcaacataat tctaacacta
     4081 ataatactac ctattattgc cgcaacatta cctattatag ctggtagtac atattcttta
     4141 ccttgagcta tcataaaagt tgttacaata agtcccatac caaaggctgt tcctaaatta
     4201 cataaagcag gtacttgata cttcttaaaa tattgtgtaa atgttttgtc tttagcaaat
     4261 ggtataatag ctggattatc agataaatat gttgcaacta ctcctgctat actagcacca
     4321 ggtaagcccc atataggtct cataagaccc ttaaatatct tattaactaa agatattact
     4381 ccaaactctg aaagcaatgc actaagtgca cctgcaagaa ctgccatagc cattattaaa
     4441 aatactgtgt ctaataaaag cgcatgtgct gtactcatta ttactttaaa catcattcct
     4501 gcacccatga tgcttcctac atatccaaat cctaccgcca atagtacaat aaatacgaag
     4561 gtttccatac ttactgcctt gactcttctc ttatccgaca gcttttccat cttgaaatct
     4621 tccccctttt aattttaaat taaggttcct aacattggta caaatttttt ggtacaaact
     4681 gagttctaaa acttacactc gtaatgcaag ttgtcagttt tttgaaataa aaaaaaatta
     4741 cgtttataga ataagaatat cctctttaat ttggatactc ttattctatt ttatgttatt
     4801 atattatcat ccttttgttt aaaagtcaat tttttctatt tttgttgttt aattttattt
     4861 atactctttt tatatttttc attttctttt atctttgtaa gtactccata taagttgact
     4921 tttctaaata cagtagttat aaataccaat cctaatgcaa ttgaacttgc acacgataat
     4981 gtactcaccc catattctac agcttttata taattattgg tcaaaacaga atacatagta
     5041 ttatatattc cataacctgg tactaatatt attaaacagc aaacacttaa tgtagttgct
     5101 ggagtcatat aaattcttgc acataactca caatatatcg caaaacaaac tgtagctata
     5161 aaaaaacata agttttctgt tactcctata tttaaaagaa atttatatga aaaccaagct
     5221 atacttccac ctattcctgc tagtattaag ttttttcctt tcatattaaa tagcactcca
     5281 aagcttaagg cagtaaaaaa agctgctatt acttctataa acaaatgcac ttatatcccc
     5341 cctaacttga agtacaatcc aagtaccaag aacattccta ctgctataga tacacctaca
     5401 aaaaatactt ctgctaattt tgcaagacca gaaatcaact gtccatctaa caaatctctt
     5461 aatgcattag tcaaagcaag cccaggaact aagagcatta ttgctcctgc tattaaatgg
     5521 tctaacgtct ttatgaatcc gagctttaaa attaaaattg aacaagaagc tataataact
     5581 gctccaattg catttacaaa aaagtcatta agggaagatt tacttaaata cataataaca
     5641 aacttagtca gaatcccaac agtaaatgca gctataaatt cttccacatt tcctccaaat
     5701 aatatcgaaa aagttgctgc tgcaatacct gcaaaaaata ttgttattaa atctgagtaa
     5761 tagttatctt cacttatttc atctaaaatt ttctcacata attcaatatc taaattattt
     5821 atacttattg ctcttgataa tgagttaact tcatgaacca agtttaggtc tactcttctt
     5881 gagcttattc ttttaattgc tgaatgtaat ttcccatcta caaatacaga tgccattaca
     5941 gctgttggag ttgcaaatac atctacgtgg tctattccaa aagattgaca tattctagct
     6001 atcgtttctt caactctata ggtttctcct ccactttgca acatagcttt acctgcattt
     6061 gaggaaaagt ttaaaattct atctatatcc acaaaacata tctcccattc ttttatagta
     6121 ttattcatat tttaatacaa gatattattt atttaaacta ttttaaataa ttcatatcca
     6181 atatttacat tttatgaaaa aacttgtttt ttatgcttgt caaaatcaaa ttttaagctt
     6241 gtttacacca taaaatggct gaattaatat ttgacaaaca ctttcaacca tttatataac
     6301 cttctaaatt gtataacttt ttactacaaa taaaattata tctcttttaa ttctttctct
     6361 tgagtattta gcttatagta acttc
//
LOCUS       DAYAOX010000210         6322 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.211, whole genome shotgun sequence.
ACCESSION   DAYAOX010000210 DAYAOX010000000
VERSION     DAYAOX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.211"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            152..1033
                     /gene="pepI"
                     /locus_tag="ACGI57_003126"
     CDS             152..1033
                     /gene="pepI"
                     /locus_tag="ACGI57_003126"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase"
                     /protein_id="HFG9718182.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1415..2218
                     /locus_tag="ACGI57_003127"
     CDS             1415..2218
                     /locus_tag="ACGI57_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718183.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESALKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            2230..3360
                     /locus_tag="ACGI57_003128"
     CDS             2230..3360
                     /locus_tag="ACGI57_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9718184.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            3364..4773
                     /locus_tag="ACGI57_003129"
     CDS             3364..4773
                     /locus_tag="ACGI57_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFG9718185.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            4990..5493
                     /locus_tag="ACGI57_003130"
     CDS             4990..5493
                     /locus_tag="ACGI57_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HFG9718186.1"
                     /translation="MFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPIYS
                     NNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRGGK
                     CEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVSSY
                     ISSHIPQ"
BASE COUNT         2580 a          559 c         1109 g         2074 t
ORIGIN      
        1 ttagtataga gatttttaac ttttatgtta tactatttat aaaagtaggt ataaagatta
       61 aaatacataa tcaaatatgt ataagaggtt ttaaatagta ttgttaatga acttgattat
      121 tgtttataat tacaaattgg aggagataaa catgaaaatt actgaaggat atatgccttt
      181 taaaggtttt aaaacgtatt accgtatagt aggagaaaat acggaaggga agaagccatt
      241 agtattactt catggaggtc caggttctac acacaactat tttgaggtat tggataagat
      301 agctgaaagt ggtagacaag taataatgta tgaccaaatt ggttgtggaa attcatttgt
      361 agaagggcat cctgagctat ttaatgcaga tacttggata gaggagctta ttgaactaag
      421 aaaacatcta ggacttgatg aaattcatct gttaggtcaa tcttggggag gtatgcaggc
      481 tatttggtat gctattgaat ataaaccaaa aggtattaag tcttacatcc tttcatctac
      541 tctttcttct gcaaaacttt gggagaaaga acaaaaaaga agaatatcat atatgagtga
      601 ggttgaccaa aaagcattac ttgatgcagt aaatactgga gattactcta gcaaagaata
      661 taatgatgca ttagaaagat ttatggaaat gtattgtgca ggtgaagtga cagaagattc
      721 tccagagtgc ctgagaagac ctaaaaaatc aggttctgaa gcatatattg taggatgggg
      781 acaaaatgaa ttttctccta caggaactct ttctggatat gaatttacag atagattaca
      841 tgaaataaaa gagccttgtc tagtaactag tggagcaata gatttatgtt caccatatat
      901 tgcaaagact atgtatgata gaatacctaa tagtaagtgg gaattatttg agtattcaag
      961 acatatgcca ttcgttgaag aaaatgagaa atatataaaa gtcttaactg agtggttgaa
     1021 tgcgaacgat taaaaactat ataaatttat ataaaaaaat gagtgtctca aaatgaactt
     1081 tttagttcat taggcactct ttttgcatga aatcaaatat attttcatta tttgaacgat
     1141 aaaatgaggc ttatatctat tttgggaaag taatttttta ttactagcgt tagattctaa
     1201 tctaattgta tacttattga aataatagaa atatattggt gtttataact taattttatt
     1261 gttttttaga aattttttta tatattggta taattattct gtttaaattt gattaaaaag
     1321 tatattaatt ataataagaa acaattatat aaataggaat agattattat aattaatata
     1381 gatataaaaa taattataga aggaaaggca cattatggaa aaattgagag ttattgaaat
     1441 gataaaaaat aatcccaata ttatagctac aatagataat ccaactgatg aaatgaaatt
     1501 gttagctatt aaagaaaatg ggcttgtact agaatatata aataatccaa ccagagagat
     1561 gcaagaacta gcaatagata ataatattag agcaattaaa tttatagata atccaacaga
     1621 agatatgatg ataaaagcag taaatgaggg atggagtata ctggattata tcaaaaaccc
     1681 aactgataaa gttattgaaa tggctataaa tcaagcagga tgggcaatta aatatgctaa
     1741 aaatccaagt gaagaattac aactgttagc agtaagaaaa aattatgatt cgataaagtt
     1801 tataaaacaa ccttatgaaa gtgtacaaga ggaggcagtg agaataagtt atgatgcctt
     1861 gagatatata gattcaccta gttataatgc agaacttata gctataaaaa ataatgaagc
     1921 agctattagc tttataactg atttagataa aaataaaatg ttggaatttt taaaagtgaa
     1981 tattttagtg attaagtaca ttctgaaaaa agtatctaaa gatgaattag agagtgcatt
     2041 aaaagaagta ttatccaaag aagatgttga ggaaaagtat attagagatt ttttgaattg
     2101 tagtgttata gatagaaata gcgaaagtat accaatagat aaaattatgt ttatttataa
     2161 atatggaagt aagacagcaa aaaaaatagc tgtagatgag aaattaaaga ttatgtagga
     2221 gagagaaaaa tgaattttat agacacatta aaaagcgttg acttaatatt atccactgga
     2281 gaagtacctt taatagtagg tgaaagtgga ataggaaaaa cagctttagc aaataagctt
     2341 gccaaagaaa atgattggag tttaattgtt attgatggaa acttacttaa agagggtgaa
     2401 ataggtggtc ttccaactat agaatcttat gtaggagtta attctaatgg atataagact
     2461 gaaaagaaaa caacagtgta tgctgttcat aacaaattaa gagaaattga tgaagagata
     2521 tctaaaagta agacagttct tttatttata gatgagataa atcgttgtga gcatacagta
     2581 cagcaagaac ttatgaattt aattttaaat agagaaatta atggatataa actgcatgat
     2641 gatgtaaaga ttttagcagc aatgaatcca tcaagtaaat atggttcaga ttttgattat
     2701 caagtggtag atatggatgc agcccaagaa aataggtttg tttggttaaa tatggagagt
     2761 gaccatacac aatggataaa atgggcaata gatgagggta ttgaaagaaa agttatcgag
     2821 tttatttcca cttttccaga atatctgcat aaaataaatg aagatgatgt aagagccact
     2881 cctagaagtt atgagagagt atctaaaatt tataaagttt ataaagaaaa aaataattct
     2941 attcctagag cagtattttt aaatgttgta aagggtaatg taggtaaagt tattgcagaa
     3001 gaatttataa gttttattga atcaaactct gaaccattaa tatcctatga agatgttttt
     3061 ttaggagaat ctattgatga gtctattgta gaaagagtaa aaaatgagag tcatacaaga
     3121 ctttatctat cagctatgaa cattctaaaa gacttagaat taaatattaa gaatgataaa
     3181 tatgaatcaa accactatat taatagattt attgaatttt taaaaatgta tcctgtagat
     3241 ttaatgatag gtattatgaa ggatattaga aacagttata ttgaagtata taaaaaagct
     3301 atagaaaatg aagagtttgt aaaatcatat tttgaatctt atagtttaat aaggggataa
     3361 tgtatggaaa attattttga taaacaagca aaagagcttt ataataaagc aagtgaaatt
     3421 ataaatactc attctatgct aaaggcaaat cgtgctaatg agaaatttga tatagatata
     3481 ccacaagatt ttaagtatga gttttttagt cttgtagata aagtgaattt aagtcttatg
     3541 gaagaagaag ataattttta tggatatttt ttgtttcaga tgtcaagaga aataagattt
     3601 gatataagta gtcctacagg tgtgaatttt aagggagcta aatatattat atattttaat
     3661 ccaataatct ttttaacgct tgacataaag caaatggaga ctacaattaa acatgaaatt
     3721 catcatatat tatctatgca tttgatgaga gcaaaggagt taaaaggaaa gtatagtaca
     3781 ttagcaatta atatggcaat ggatatagta gtaaataaat ttctaaacaa tttaccacca
     3841 tatgcaacta ccttagagtg ggtaaattta aaatattcct taaaacttga accatatgaa
     3901 ccttttgaat attatgtaga aaagattcag actgaattag atttgatgga agtggatgaa
     3961 gatggtgagg aagatgatag tgataaatat ggagatatag aaattgatta tagccccgaa
     4021 agaactcatg atatatggga tgaatccaat gagatagatg aaaataccct tagagaattt
     4081 actgaaaaat ttattaacag ttctcaaaaa ggtaaagttc ctgcttattt agaaagtatg
     4141 atatcatcac ttaaaaatag taagggtgaa ttgccatgga atttatatct taatagaata
     4201 atgggaacag ttgaaaataa taaaaagaag actataacaa gaagaaatag aagacaacca
     4261 aatcgtttag atttgagggg agaacttaga agtcataagt cagaaatagc agttgcactt
     4321 gatgtaagtg gaagtattag tgatgaagag tttaaacaag ctattaaaga agttcttagt
     4381 atagtgaaaa actataatca tgaaattaca attatagaat gtgataatga aattagacgt
     4441 gtatataaag tgagaactga aaaagatata aaagatagaa ttaggatagg tggaggtact
     4501 agattttctc cagtctttga gtatgctaat aataaaaaga ttaatttgtt agtctacttt
     4561 actgatggta aaggtgaaaa taaactacaa gtaataccta gagggtacaa aattttatgg
     4621 gttatttctg gaagaggaga taaaatttca ttaaaagaac cttatggagc agttaaaaaa
     4681 cttactaagg ttaaaacaaa tgatgaaata ttagaaataa atgatgtaag aaatgatggg
     4741 tactcaatga atagccaagc accaatttta taggtattca caggagataa gctgtattaa
     4801 tttattttga tatgcttgtt ataaaaatct aaaaaataca ttaatcaatg gttaatgtat
     4861 tttttaattt taataatata agttttactt acatttaagt ctcaaaatgt gttaaaatat
     4921 aatatatagc agatagtttt ggcaaggtgg atttagttgt caaaactatt ttttttatta
     4981 aggatggtga tgtttttgtt taagattgat gattatataa tgtatggtat gacaggagtc
     5041 tgcaaagtca tggatataac aaatgaacga tttacaaatg gaataaaaaa agagtattat
     5101 gtattgagtc ctatttattc taataataca gtaataaaaa ttcctgtaga taatgaaaaa
     5161 gtaccaatga gaaagttact ttctaaaatt aatgtgcttt cattaataaa tgatattcct
     5221 aatatggata catcatggat agataatgaa aaattgagaa gtgagcaatt taaaaaaata
     5281 ctcagaggtg gtaaatgtga agagttaata aaattagtaa gaagtataga taataacaga
     5341 gagtatgtaa aatctattgg taaaaaaaca catcaagcag atgataatat aatgaaagaa
     5401 gctgaaaggt tattaaatga agaatttgca actattttag atatttctcc taatgaagtt
     5461 agttcatata tttcaagtca tatacctcaa taaagtgttt tagtatcctt tatttaatct
     5521 attttaagta ttatttatgt atgctggttt tggtaaaata aaatatataa ttaaattata
     5581 taaaaaataa taatgattgt attgaagaat tacagaatag tgcggagagc ctactccttt
     5641 aggtgtgaga tggatatcac ttaaagattt aaattgttat tgtagatgtt tttaatataa
     5701 aacaagatta ataagtgtat tgtaagcttt aagtgaaatt gatactataa tgagttactt
     5761 agattttaaa atatataata ttataaaaaa tccttttagg ttttgaatga ataagatgga
     5821 tactatgttg aatagaatcc atcttattta agttttattt atgttgttta tttgtaagca
     5881 ttatttttgt acatgaaaac acagtgtaaa aatatgctta gaattatgaa gttttattaa
     5941 aaagatattc gacaaaataa gactaaaaat attcttaaac ttataactaa gataaatatt
     6001 gtatatttat agtgtagcct gaaaaattgt ggatgtacga aatttattta gtattttatt
     6061 gaaacaaagt agaagaagta ctatagttta tctttgaata gattgttatt ttttagtgaa
     6121 aaagttatta ggttaataca taaatataat agcttataaa tagtttaagc atgttaaaaa
     6181 agtagttatt agagatatat agttacattg cttataagat acttaaaatg atagcattca
     6241 cttatattca gaagattaag ataaaaaatc atttttatat agtaaagcat acctaaattt
     6301 agaaaataat tgaatttata aa
//
LOCUS       DAYAOX010000211         6310 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.212, whole genome shotgun sequence.
ACCESSION   DAYAOX010000211 DAYAOX010000000
VERSION     DAYAOX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.212"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            438..1805
                     /locus_tag="ACGI57_003131"
     CDS             438..1805
                     /locus_tag="ACGI57_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFG9718187.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            1830..2438
                     /locus_tag="ACGI57_003132"
     CDS             1830..2438
                     /locus_tag="ACGI57_003132"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFG9718188.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            2486..2857
                     /locus_tag="ACGI57_003133"
     CDS             2486..2857
                     /locus_tag="ACGI57_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFG9718189.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            3036..3374
                     /locus_tag="ACGI57_003134"
     CDS             3036..3374
                     /locus_tag="ACGI57_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFG9718190.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            3840..4343
                     /locus_tag="ACGI57_003135"
     CDS             3840..4343
                     /locus_tag="ACGI57_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HFG9718191.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            4390..5349
                     /gene="moaA"
                     /locus_tag="ACGI57_003136"
     CDS             4390..5349
                     /gene="moaA"
                     /locus_tag="ACGI57_003136"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFG9718192.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            5353..5823
                     /gene="moaC"
                     /locus_tag="ACGI57_003137"
     CDS             5353..5823
                     /gene="moaC"
                     /locus_tag="ACGI57_003137"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFG9718193.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            5859..6290
                     /locus_tag="ACGI57_003138"
     CDS             5859..6290
                     /locus_tag="ACGI57_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HFG9718194.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         2652 a          510 c         1132 g         2016 t
ORIGIN      
        1 tagtggtaat ttgttttttg tatttaaaaa taaatgtatt tacttgtatt ttgaattaat
       61 atctacttag acattgtttt aattgttata tttttattta attaagcatt gatttatcat
      121 tttttatatt tcatataaca atgcaaaaaa taatttttat atagtaaatg acaaaagttt
      181 acaataaatg tatattttct tactcattcc ttaaaattac tcatttagtt taataactct
      241 catttcatat gtaaagttgg taaaaaaatt aaaattaaaa gtaaaaaact aaataaatta
      301 atgttaattt gaaatatata tgaaggtttt tggagaatac ttacttatga taggaaaaaa
      361 tgttaaaata atagtgtaat caaatataaa ggactgaaat aattaacagt tattaaatat
      421 taggagtgta aaaatttatg ggattaaaag aatttgattt tattgaagag tctattgata
      481 tgttgaggac aatgtcacca acattagaaa ctatatcaga tgaaatagaa gaatattttg
      541 aaaatatctt ggatgaaaag aatcaagaat atataaatgt aacctctaga ataaaatcag
      601 aatcaagttt aagagaaaag ataattagaa atagatattt aaaaaaatat ggagaagcaa
      661 gtaatcttat tcataatgtt tctgacttaa taggcctaag aattgagtgt agatttattg
      721 aggatgaaaa caaaatatat agacttttga gaagatattt taataaaaca gatgacaaaa
      781 taaactatta taataaagag aataagaata ttaaattaaa actatctgag aggcaaccca
      841 ataagcaaaa aaatggattt gaaatatata aaatagatgg agtttttgcg tatttagata
      901 gagaagtaaa atttgaactt caaattaaat ctcttgtaaa tgtgttttgg agtgaaatag
      961 aacataaaat aatttataaa aacaatacat atttacttgc tgataaattt ataaaggata
     1021 tgatggactc tataaagaat aatcttacta tgatagataa tcagctttta agtatttata
     1081 aaaactttca ttcagggaag tcttttaata tgaaagtaag taaaaaagaa attgaaaaat
     1141 tatttgcgaa actagtctat gacgcttttt ctgaaaagat gaataaaagt ataggttttg
     1201 ttgtagattt taaaaaacca tgtgagacta tattgaacta ttcttttaat aaacaagata
     1261 taagtgatga agttttaggt aattttatgc ttgatgagtt tgcaagatta aatgaaattg
     1321 taaacaagga tatagatttt aatgagcaaa tagaatttga aagagaacca acatttgatg
     1381 ataagttttg taaagaccta ggaaatcatt ttagaagtag attaaacaca gaatttccat
     1441 ggaacctatt ttttagaata ttatttgaaa tagagccata taacaatacc aaagactttg
     1501 aaaactttgt agagtttata aaagaaaatg tgctactaga agaaaatagg gagcaattat
     1561 tatgccaatt tgaagaggat agtaagctta taattgaaga tatatatgat tgtatattta
     1621 atagtataag tgagattgat agtgttgaaa ttttatataa ctataatctt gaaaaaataa
     1681 actttacatc aagtgaaatc ataaattgta tatgtagaga atatgagtgt tatggtgaat
     1741 acttagaaga aaaagaaaag cttatgaata catttaaaga aaagattatt caaatctttg
     1801 agtaataggt caatgctgaa gtggcgctaa tgcgccattt ttcaaatata tatgaattaa
     1861 gtaaaattat tttggaggag gcgatttgta tagagaaaac aggtgtaatt ttggcagggg
     1921 gaaaaaattc tagaatgggt agagataaag cctttttaga gttacatgat aagcttttta
     1981 ttgaaattgc aatagaagca tttaaaaact ttgatgagtt gataataata tctaacaatg
     2041 aagaattata ttctaaatac gatattaaag tttataatga tatagtaaaa gatgttggtc
     2101 caattggagg aatatatact gctcttgaat atgcaaagta tgatatagtt acaattgcat
     2161 gtgatatgcc atatctaaat aatcaaatag tcgaaagaat agctaataaa atggatgata
     2221 aaagtgtgat ttcagtgaca aatggaaagt tacaaccatt gtgttctgga tataaaaaga
     2281 gtataataaa taaagtatct ttatgtataa gggaaaatga tttaaagctc agaagcttta
     2341 tagataaaat agataaaagt tatatatact ttgatgaaga agatttattt ttaaatgtaa
     2401 acactgttga tgagtataaa aaattaacta aagtttaatt agctatggtt gtaaaataga
     2461 gtaggattta tatggaggag agtttatgaa tttacttaaa tgcgattctt gggcagtaat
     2521 attaaataat agcgataaag aaagtaaagc atataaaata ttagatgaac ttaaaaggaa
     2581 tatgtacaaa gttgttgcta tagatgaaga gaaaaaacct attgaaggaa tagatgtgta
     2641 tgaatgttta aaagatgttc cacacaatat tgatgttgta gctattattg ataaacaatc
     2701 taagatggat gtgattttag aagaagtaga gcttttggat atacaaaata tgtggtttga
     2761 aaaaggaagc tttagtgaaa tgatgattaa aaagactaaa gatttaaaat taaatataga
     2821 atataattta agtttatatg atgagttgac tagataaaat tatatttctt aataataaaa
     2881 atataattag ttaataaaac atattatttt atagtataga ttggttaata aaagtacaga
     2941 ttggttaatg aaacattaaa ttacaatatt tgttaaaaaa attgtgagtt acatgatata
     3001 atgtattaaa taggatacta aggagttgat atattatgtt aatagtaact acagaaaaag
     3061 tagaaggtaa aaagatatca aaggttttag gattagtgag aggaagtaca ataagagcaa
     3121 aacatgttgg aaaagatata ggagcaagtt ttaagaatct tgttggagga gaacttactg
     3181 gatataatga aatgctcact gaagcaagac aaattgccat aggtagaatg gttgaagatg
     3241 cagaagctaa aggtgcaaac gcagtaatag catttagact gtcttcagct tcagttatgc
     3301 aaggagcagc agaaatgctt gcttatggaa cagcagttgt tttagaagat gataatagta
     3361 ttcttgaaaa ataaaatata aatagttaaa aagaggcatc ttttaaaaat tttatggatg
     3421 ccttttttta tttgaaaaaa ctaatttgag aagaaatttt aactattatg acaataaaat
     3481 tagggtgata ttatataaat aagtaaaatt atattatata atgtatggta tagactttaa
     3541 aaattttatt tgtagagaaa aatatggaca atgttataat aattatagaa attaaaagta
     3601 cttacattca ttatgtatgt gacatgtata attagtttta tatttaatac agttttgaag
     3661 taatatcttc attttgagaa cgttggaata ataaaaaatg tattattata ggtgatataa
     3721 ataaaaaata aatataatat gttatattgc tgatagaaaa gagaataaaa gaaaataggt
     3781 tttattaaaa gagattttta tttataggac ttaatcaatg tatagagggg tgagaacata
     3841 tgtttaatgt tgcaattata acacttagtg ataaaggata tgaaggaaaa agagaagata
     3901 taacaggaaa aaagttaact gaatttgttg aaaatacagg agtttataaa gttactgaat
     3961 atgtacttat aaaagatgat aaggaaatgc taaaggaaaa tattataaga ctttgtaata
     4021 gtaataaaat agatttaatt cttacaaatg gaggtactgg tttttcaaaa agagatataa
     4081 ccccagaagc aactaaggaa gtgttggaaa aagaaatacc aggtctgagc gaatatatga
     4141 gaatgaaatc cacagaaatt actaaaaagg ccatactaag taggggtgtg agtggaataa
     4201 gaaataattc tataataata aatttacctg gaagtcctaa aggtgcagtt gaaaatctaa
     4261 gttttataat tgatgtttta gaccatggca tagaagtttt aagaggagaa gctacagaat
     4321 gtgccactaa aaaaagtgaa tagtatataa tctagataga taaaaaatag acaaagaggt
     4381 agataaagta tgattgataa gtatggaaga aaaatagact accttcgtat ttcacttaca
     4441 gataagtgta atttaaggtg tgtctattgt atgccaccag atgtaaaatt tgataaaaat
     4501 tatataaatg aaaatctaag ctttgaagac tacaagttta ttataaaggc tatggctgaa
     4561 caaggtataa caaaaattag atttactgga ggagaacctc ttttatatcc taaattaaat
     4621 gagcttataa aatttacaaa agaagaatgt ggaataaata acataggaat gaccactaat
     4681 gctattggtc tttctgatag aatttttgag cttgaaaaat gtggcttaaa taaagttaat
     4741 ataagtttag attctttgaa ggaatataag tataaatcca taacaagagg aggaaactta
     4801 aaagatgtct taaaatcgat tgaatcttgt ttaaatttag gtataaaagt aaagttaaat
     4861 tgcgttgcta taagtgggtt taatgatgat gagcttaaag attttatgtt tttaacagtt
     4921 aaatcaccaa ttgatgtaag atttatagag cttatgccat taggtgaggc taatagagtt
     4981 tataagaagg gttattttaa tgtaaaagaa atgattgaaa aaatatatgg tttatataaa
     5041 gtcaaaaatg atgaaaaaag tacagcagaa tattatatgt ttaaaggagc taaaggaaga
     5101 ataggaatta taactccatt aagctgttca ttttgtaaca cttgtaatcg tattagactt
     5161 acttctagtg gtgcaataaa attatgtcta cattcaaaag aagaaataga tataaaacca
     5221 tttttacata aaccattgtt atttagagaa tctatgaaag atataataaa acaaaaacca
     5281 gaaaaacatc atttaataga aaataaatgt agtgatacaa ataggcgtat gtatgaaatt
     5341 ggaggataag taatgttaac acatataaat gaaaaagggt atgccaagat ggtggacata
     5401 agtgaaaaag atgatactaa aagaacggcc gtagctacag ctacaattac tcttagtaag
     5461 gaagcattag aaaaagtaaa aaatggtcag ataaaaaaag gagacgtatt gtctgtagct
     5521 caagttgcag gtataatggg agctaaaaat acatcttcaa acataccaat gtgccatcag
     5581 attaaattag ttggatgtga tttagaattt gatgtagatg attctatgtc tgctattaag
     5641 atatacgcaa cctgtaaatc tcttgggaaa actggagtag aaatggaagc tttaaatgca
     5701 tgttctttag cagcacttac aatttatgat atgtgtaaag cagttgataa aaaaatggtt
     5761 ataagtaata tccatcttgt ggagaagact ggtggaaaat caggagattt taagttcgag
     5821 taaattaata atatattggg ttaaaatttg gaggaaatat ggcgaaggta gtgtcaataa
     5881 atataagtga gaaaaagggt gtaattaaag ttcctgtaca atgtgcagag ctaaaaataa
     5941 atcatgggat agttggagat gcacatgcag gaaactggca tcgtcagata agcttacttg
     6001 gaaaagaaag catagataaa atgaaaatga aaggctttga ccaattaaaa tatggagatt
     6061 ttgctgaaaa tgtaactaca gaagggatag aagttttttc cctgcctgtt ggtacaaagt
     6121 taagaatagg gcaatgtgaa gttgaagtaa ctcaaatagg gaaaaaatgt cacaatggat
     6181 gtgaaattaa gaaattgaca ggtgactgtg tgatgccacg agaaggaatt tttgtaaagg
     6241 ttctaaaaga aggaattata aatgtgaatg attcaataga tgtaatctaa tgaagtcact
     6301 attaaaatat
//
LOCUS       DAYAOX010000212         6308 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.213, whole genome shotgun sequence.
ACCESSION   DAYAOX010000212 DAYAOX010000000
VERSION     DAYAOX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.213"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            288..3386
                     /locus_tag="ACGI57_003139"
     CDS             288..3386
                     /locus_tag="ACGI57_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9718195.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVTLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3509..4795
                     /locus_tag="ACGI57_003140"
     CDS             3509..4795
                     /locus_tag="ACGI57_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFG9718196.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(5174..6280)
                     /locus_tag="ACGI57_003141"
     CDS             complement(5174..6280)
                     /locus_tag="ACGI57_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9718197.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGEIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
BASE COUNT         2466 a          691 c         1039 g         2112 t
ORIGIN      
        1 tcttcatata tgattggaat acttaattga ggtggagatt tgtcatttat atgagaatta
       61 attataaaca cataatgaga taattaatta taaatatatt aaattaggta ttacaaaact
      121 ctattgtacg attgacacaa taataattct atttactaaa aaatagaacc tcattaaggt
      181 actattttaa aacataaaga aatataatat cattgccaaa tatatagcaa aattttaaaa
      241 gctttaaaaa ttcaatctgg aaaatgaata aaaggagata tatataaatg gatgtaataa
      301 atttaataga tgagttggat gaaattgtat atgtttcttc tttagataca tatgaacttt
      361 tatatattaa tgaaattggg aaaaaggcaa tgggacttaa tgatatatcc catttaaaat
      421 gttataaagc attacagggg aaagattctc cctgcgaatt ttgtacaaac tctattttaa
      481 aagaagatga atattacata tgggaaaata cgaatactcg aacaaataga cattttatat
      541 taaaggacaa gttaattaaa tgggagggca aacatgcacg attagaaata gctcttgata
      601 ttacagaaaa ggaagatata agtaagtcaa tagcagaaaa attagagata aaggagttat
      661 tagttggatg tattaaacat cttataacag aatctaattt ttctgatgct gtaaatcttg
      721 tattaaccaa tattggtaaa tttcataaag ctgatagagc atatgttttt gaagtatcag
      781 aagatagaaa aaaagtaact aatacatatg aatggtgcaa tgaaggtgtt gatgaacaaa
      841 aacaggcact aacaaatctt gatattaatg aattaaagaa ttggttttta ttattcgaac
      901 aaaaaggttt tgtaataatt gatgatatag aaaatataaa agaagaacag ccaatagaat
      961 atgaaatttt aaaacctcaa aaaattgaaa gtttaatgac atcttcacta aaacaagatg
     1021 ataaagtagt agggtttatt ggagttgata atccacataa agctgttgga gatacctctc
     1081 ttttgacatc cctatcttat tttttattaa ctgagatgaa aaaaagaaaa gtagaaaata
     1141 aattaaatta tatgagctac tatgacttct tgactggtgc atataataga aataaatata
     1201 ttaagtatat agataatttt caaaaaggaa aagttaaatc cataggagtt gtatttgtag
     1261 atataaatgg gttaaaagat attaatgata actttggaca tgctaatggc gattctgcta
     1321 ttgtgtcagc atgtgtaata ataaaaaaat actttcattc ttataatgtt taccgtattg
     1381 gaggagatga gtttgttgta ttatgtgaag atatatcaaa acaactattt gaagaattag
     1441 tgagttggtt aagtgaagaa tttaaggatg agatgatata tagtgtagca gtgggcagta
     1501 tttggagtga cgataatatt gatatacata agctaattaa agttgctgat gagagaatgt
     1561 accaagataa gaagaggtat tataataaga acaaacttag aaataatgaa atgaaagtaa
     1621 ttagtgactt agctgattcg acgaataata ttttatttaa acataaagag ttagttgata
     1681 aggtttattt tgatgattta atgataaaag ggtattctaa ggcttatgaa aaattagctt
     1741 ataatacata tgatataatt ttatctattt gcattgatac gaatttaatt actccaattt
     1801 atcaatcaga gagcaatatt tttagttttc ctataaagga aaatcttgaa aaaacttttt
     1861 catacataaa aaaacattta attcatcctg atgatataat attatttaat gataaccacc
     1921 ttctaaaatc tcttgatagt cttaataata gcaagcaaga cgatttagaa ctaaattttg
     1981 agtatagacg tttaggaata gataaaaagt actattgggc attaatttat atgttaaaga
     2041 ttagtagtaa aaataagtta ggtgagaaac aaacacatat tttagttgct ataaaaaata
     2101 tagattatct tatgcaacta cataaaaacc aaaaagatac attaacagga ctatacaatt
     2161 atgaaagttt ttgtgaacaa ataagtttaa tgcttaaaag atatcctgac aagcgttatg
     2221 caattataat gatggatata gataagttca aggtaattaa tgacatttat ggagttagag
     2281 gaggagataa agctttaata tatgttgcag agttgattca aaaaaacatt ggttctaatg
     2341 gtatatgttg tcgtatgtat gcagatgtat tttgcatttt ttatgagagt tactctgata
     2401 gagatattca taaggtaatt tataagatta aaaatagctt aaatagaaaa aaatttgaat
     2461 atatgttagt accatgtttt ggggtttata gaattttgga taatcatatt ccaattacaa
     2521 atgcgtgtga aatggcagga tatgctcaca aaaaagtaaa gaaacaaagt atcaataact
     2581 atatatttta tgatgatact ataagaaatg atgcacttga agaaaaacag atggaaaggg
     2641 aaatggaata cgcacttgaa aatgggcaat tccatgtata tttacaacct aagtataatc
     2701 ttaatgcaaa taaaattgtt ggtgcagaag cattggttag atgggttcat ccagaaaaag
     2761 gtataatacc tccaattaaa tttatttcac tatttgagaa gaatgggttt attatcagat
     2821 tagatatgta tgtatgggaa caagtatgtt ctttgattag aaaatggata gatttaggtg
     2881 aagagccagt tcctatctct gtaaatgtat caagattaca tttgcataat ccacatttta
     2941 aagaaattgt tttaagttta ataaataaat atagtatacc aaaatctttt ttagaattag
     3001 agcttacaga aagtttattt attaaaaata taaaattatt tactaagata atagccgacc
     3061 ttagaatgga tggtattgtt ttaaatatgg atgactttgg ttcagcatat tcttcattaa
     3121 atatgttaaa aaatattaat attgatacat taaagataga ttgtggtttc tttaatgagg
     3181 aaggaatcac agacagagaa aaaatagttt taaagcacac tatagccatg gcaaaagatt
     3241 tagatatgga tgtagtggca gaaggtgtag agacaaaaga acaagctgaa tttttagtga
     3301 ctttagattg tgtggtaatt caaggttatt attattgtaa gcctattcca agtgctgatt
     3361 ttgagaagtt actttatcaa aaataagcta attattccat tctaaatgta ggtaggatgg
     3421 agtagttcaa aataattaaa tagaagtaga aaatagtgag gatataattt ttaaaaagta
     3481 taatatttat ggattaggag cgaagtttat gatagtgggt attataggtc cttcagactc
     3541 tgggtttaag ataaaggatg atttaaaaca gatagacagt gatttaaaga ctaaaatata
     3601 tgtaagagaa aaagtagtag atacaataga agttatttct aaatgtgaag acgaatgtga
     3661 tgctattata tttacaggat gtgcagttta tgaatttata aaatctaagt atgagataag
     3721 taagccacat tcatttgtac cgagaagtgg gacgagtata atgaaggcat tttgggcaat
     3781 aaaaagtgca aatataaaat tagataaatt cagtatagat gtagttgata gatatgttgt
     3841 ggaagatgca cttaaagaat ttgatattaa agcaacgagt gtattttgta atccattttc
     3901 tttggaagta ggagagtctg aacttgtaga atggcatata aaattatttg aagaaaataa
     3961 gacggatata atgttgactg gttttggtgc agtatataat gaattaaaag aaagaggcta
     4021 tcctgtattt cgtttacaag ctactataca attaatcaaa gaaagttatg acaaggtaaa
     4081 gagtgaatat gctctaagta aggcaagatt ttcccaaatt gctgttgaaa tattgagttt
     4141 aatagattat aaagagaaga tagataacta ttattctgac atgataaaaa aatcagatat
     4201 ggataagctt gtagtaaact atgtaagaag cattcaaggt tccttatttt tacttggaag
     4261 aaatgattat gttgtatttg ttcataaagg ggtagctgat aatgagtata attatgataa
     4321 gctattcaat ctaaagagag agataaaaga catgggcttt tctcttagta taggaattgg
     4381 tactggagta actgcgtatc aggctgaaaa taatgcacat aaagcattaa gacactccat
     4441 agattcaaaa gaaattggta tatatttggt agatgaagat gaaaacataa gaggaccttt
     4501 agcttccgaa aatgaattaa attattcttt gatgctatct gatgaaaagg taatcgaaat
     4561 atctaaaaag actggaatga gttgtgagtc agttgcaaaa attattgcta taaatgagaa
     4621 tagaaaaagt aagatttatg attcaaaaga gttagcagag tatctaaatg taagtgaaag
     4681 aagtgcaaga cgtatattaa ataaaatagt aaatgcaggt ttaggtcgaa tatgtgcaaa
     4741 agagactagt attggtggag gaagaccaaa aaatattact gaaatactgt tttaagatat
     4801 tttataaaac tgtataaatc attatattgt ataaaattct acaacaaatt actatataaa
     4861 gtattaaagc ttattatgaa aaaaattaga acaaataatt atgtaaaata ctagaataaa
     4921 ttattaatat aaaatactag agcctattat taaaaatgct agaataaatt attatgcaaa
     4981 atattgaagc ttattatgaa aaatgttaga acaaattact atataaaata tcaaagctta
     5041 ttatgaaaaa tgttaaaaca aattattata taaaaatact aaaacctatg taaaacaact
     5101 ctaatatata acttataaag ttgaggatac ataggttttt atttttataa tttaaagagt
     5161 tctattatga tatttataat ttaatcatat gataaactat tttggcagta tcatataatt
     5221 cgtctatatt tacatattca ttaactgtgt gtacatcata catacctaga cttaatatag
     5281 cacaattata tccaagatta gctaatatat ttgcgtcact tccaccacca attaccattg
     5341 gatttggaac aacaccaact tgtcttattg cttcttcact taacttgaaa acatgactat
     5401 ctctactaag ttcaaaacta gggtaagaca tattatgttc aaaagtataa gtagtattaa
     5461 atttagatgc agcgtcctta caacattttt ccatatgatt aagctcatac tctaaagttt
     5521 caggtatatg agacctaatt tcagcagtca atgtaacctt atcagttact atgtttgtag
     5581 ctccaccacc ttctatttca cctatatttg aagtagtcaa tgaatctatt ctgcctatgt
     5641 gcatgttgct tattgcatga gaggctacta atatagcatt taaccctttt tcaggttcta
     5701 taccagcatg tgctttcttt ccttgaaaag agatttttat agattgttga gcaggagctt
     5761 tgtatgctat cgaaccaata gctccaccag aatctaaaat caccatatct ttacaaggca
     5821 agttgtcagg attaaagttt ttgacaccat gcataccagc ttcctcacag attgtaaaac
     5881 aaagataaat atctctatgt ggtatttttt ctgtaataac atgttctaaa gcttcaagta
     5941 tagcagctat tccagcttta tcatctgcac caagtgtagt agttccatca cttctaacta
     6001 cattaccatc tagtattggt tttacaccta aacaaggttg aacttggtcc atgtgtgcac
     6061 ataaacaaag tggtctactt ccttcttcac cttttatata ggcaatgaca ttacctgtat
     6121 taccaccata tttttcacct gcatcatcta taatggcatc tatatttctt tcttttaagt
     6181 agtttaccaa ccacttagac atttctaatt cttgattaga tgggctatca attctaacca
     6241 tatccataaa attatttata agtctatttt tatctaacat aaattatttc tccttttaga
     6301 aagattta
//
LOCUS       DAYAOX010000213         6187 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.214, whole genome shotgun sequence.
ACCESSION   DAYAOX010000213 DAYAOX010000000
VERSION     DAYAOX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.214"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..410
                     /locus_tag="ACGI57_003142"
     CDS             <1..410
                     /locus_tag="ACGI57_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HFG9718198.1"
                     /translation="IANMFMKKDINKEFDIDFILSKEVICNYTKEIFQNLYKKEQSIG
                     KKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKIYLFRKFGVPFVVG
                     CLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(710..1297)
                     /gene="rbr"
                     /locus_tag="ACGI57_003143"
     CDS             complement(710..1297)
                     /gene="rbr"
                     /locus_tag="ACGI57_003143"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HFG9718199.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(1383..1820)
                     /locus_tag="ACGI57_003144"
     CDS             complement(1383..1820)
                     /locus_tag="ACGI57_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9718200.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            1924..2922
                     /locus_tag="ACGI57_003145"
     CDS             1924..2922
                     /locus_tag="ACGI57_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HFG9718201.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(3042..3968)
                     /locus_tag="ACGI57_003146"
     CDS             complement(3042..3968)
                     /locus_tag="ACGI57_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFG9718202.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            4353..5957
                     /locus_tag="ACGI57_003147"
     CDS             4353..5957
                     /locus_tag="ACGI57_003147"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFG9718203.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         2304 a          767 c          958 g         2158 t
ORIGIN      
        1 atattgcaaa tatgtttatg aaaaaggata ttaataaaga atttgacatt gattttattc
       61 tcagtaaaga agttatatgt aattatacca aagaaatatt tcaaaattta tataaaaaag
      121 aacaaagcat aggtaaaaaa gaactgctta tattgagcat aatagagaag tttttagtta
      181 aatctataat aacttttgaa gagttaaaat tgatagtgaa tataatagaa gaaaatttta
      241 catcaatata caatgaaagt ggaaagattt atttgtttag aaagtttgga gttccattcg
      301 tagtaggatg tctggattat aaaaaagtaa agtttgaaaa agatgtagtt aagattattg
      361 aggttgactt gacaaaagaa ataaatgata taagtataaa gttatcatag tgtattatct
      421 tatataatgt attataataa tctacttaaa atacacttta tttaaatatt tagtttaaag
      481 atgagatttt atgtgagtgt gtaaaagtta aagatagatt taagtaggtt atagggatta
      541 gtaaacaata aaatgaacca tgatgaacta ctataaattg tatttaaact acattttcta
      601 cttatagtaa tatcaatggt tcattttatt gttattaaga taatacagta tttataaaaa
      661 tacttgatat aaatatagta aattgtttaa atagaattat tgccttaatt tattcaaata
      721 atgagttttt aaaatctaag aaatatcctt gtggatgaag acatacagga caagctttag
      781 gagcttcttt acctatatgt atatggccac aatgtgtaca tatccattgt atatcttcat
      841 ttcttctgaa taaagtacct tcttctaaat cagtagcata tctaccaaat ctatcaccat
      901 gaactttttc tatttcagct atattttcaa ataacttagc tatagattca aatccttctt
      961 cacgtgctgt ttttccaaag tttttatata catcatccca ctcttgaagc tcattatgtt
     1021 gtgcagcttt aaggtgttgt gctgtcttgt catataaatc tacagggtat gctcctgata
     1081 tacttatatt ttcaccagca aattctttta atttagaaaa gaattgtttt gcatgtgatt
     1141 tttcttggtc agcagtgtaa ttaaataaag actctattat atataatcct tctttttttg
     1201 ctacagatgc agaaatattg tatctatttc tagcttgact ctcacctgca aaagctctta
     1261 ataaattttc tttagtttta ctatctttta aattcatatt ctacctctct atcacaataa
     1321 atttttacaa tatttgattc tttttattta ttattccaat aggagatata attatgctca
     1381 atttagtcag catttttaaa attatcatta atttttttta aaattctaag aaattcttgt
     1441 ttttcatttt ctgtaatatt atggtatgcc gtctctttaa cttgtgttga gatgctatta
     1501 aaagtatctt ctataagcag tgcctttttt gtaagcttta tatacgtgat acgtttgtca
     1561 tttgtacatt tttgtctttc taaatatcct aggtttataa gtttattaac caacactgta
     1621 acagtggatt tatcttttcc tattttagct gcaatttctt tcattgtaag tattccatta
     1681 ttttcataaa gtgcagttaa aatatttcca tgagtaggta ttaactcacc taaaccattt
     1741 ttactgagtt tattttcaat gaatttaatc atactagcct ttgttttact tatgaaatgt
     1801 acaatatatt tatcttccat aaattattct ccaatcttta atctatttaa attagtttga
     1861 cataaaacta tttataaact ataattagtt taacataaaa ctaaatgtat tgggaggaaa
     1921 tttatgaata aaaaatttaa atcactatat attacagaag aagaaggaaa ttttagaaga
     1981 aatatagttg aaagagaagt ggatacattg ccagaaggcg aagttctaat aaaagtaaaa
     2041 tattcttctc taaattacaa ggatgcactt tctgcaaatg gtaataaggg tgttacgaga
     2101 aagtatcctc atacaccagg aattgatgca tctggtatag tagaatctag tagttgtgat
     2161 gattttaaaa ttggagaaga agttttagta acaggttatg acttgggaat gaatacttct
     2221 ggtggttttt cagaatacat aagagttcct tctagttggg tagttaaact tcctaaaaat
     2281 ctttcactaa aagaagcaat ggattatggt acagcagggt ttacagcagc tttatctgtg
     2341 tataaattta tagattctgt agacaagaat atgggagatg tattagtcac tggtggtact
     2401 ggaggagttg ctgtaattgc aactaaaata ttgattaaat taggttattc agttgtagtt
     2461 tcaacaggaa aattagaaga acaaaaagaa gtattactaa acttaggtgt taaagatgtt
     2521 atacataggt cagaagtgga tgataactca ggaagacctc tgcttagacc aagatgggct
     2581 ggagtaatag atacagtagg aggaaatact ctagcaacag caataaagac aactaattat
     2641 tgtggagcag taacaacttg tggtaatgca ggaggagttg attttacaag ttcagtatat
     2701 ccatttatct taaagggaat tactttatat ggtatagatt ctgtggattg cccaatgaac
     2761 ttaagattaa aaatatggga tttattatca aatgaatgga aattagatga tttagatgat
     2821 atggtaaatg tagtatcatt ggaagagtta aatgatagta tagattcaat aatggcagga
     2881 aaacatgttg gaagaacagt agttgattta gaaaaaatat aggtgaataa atttataatt
     2941 aggattatag gttttaagca aaaatgagat acttgaaatt tacttgtgag tatctcattt
     3001 ttttatgtaa aaagatttat gtagatgttt gtgtaaaaag attattcatg aacaattaat
     3061 tcaaatacat ctggtcttga atagtgacct tctgaatcaa aatctagtct actaagtact
     3121 attttttcta ggtctaaatc agctattagc atttcttctt tattgaaaat aggacctgct
     3181 acatattttc caaaagggtc aactatacaa cttcctcctg gacacataat ttctggttct
     3241 gcatctagct ctttatagta gttaaggtca gttggataca tatttttttc aacatattga
     3301 ttgcatccaa ttacaaaaca tcttccttct aaagcaatat gcttcatagt tgcttgccac
     3361 tcttctcttg aatctgcagt aggggctatg taaagtttta cacccttagc ataaagagct
     3421 gttctagcta atggcatata attttcccaa caaatcaacg aacccatttt tccatatgga
     3481 gtatcaacta ctgtaagagt acttccatct ccttctcccc aaatacatct ttctgttcct
     3541 gtaggtttaa gttttctgtg tttaccaatt agcttacctt ctggagagaa aaataagttt
     3601 gtgcaataaa gggtacaatt tatatcatta ccatctcttt ctgttatacc cattgataga
     3661 tatactcctg cttcttgtgc tgcttttcct aataaatctg ttgtagcact aggaactggt
     3721 acagaattat catagtatct tttccaatct tctctacctt ccattgttct acttccaaca
     3781 acaaatccaa atgaaagacc tcttggatat gcaggtatga atgcttcagg gaaaactacg
     3841 atatttgccc cttttctacc agcttcttta attaatccta atgctttttc aacagttttt
     3901 tctaaatcca ttatgactgg tgacgcctgg acaacagcaa ttcttacatt tttattttcc
     3961 ttgctcataa aaacctccta aaaaatattt aaattttttg cattgaatag attacatgag
     4021 atgttatttg agaactaaga tacttatatt gtgaataata atgtaactgt atatttaaaa
     4081 taatacatca tgatttaaaa aattacaatt gtacagatat aattgagttt agatatttaa
     4141 atttaacaac ttataatatt ttttcaatta gtataaagcc tattaatttg tcttatttac
     4201 aatgaatagt atttacttct tggcaaacaa taattaaaaa taaaactttt atatgattaa
     4261 actctaaaga aaaagtttat aaagaagata atataattaa gcagaaaata atgaaaaaaa
     4321 tttattaaag tagacaacgt aaggagaatt ttatgaaaga aaaaatttat tacaatggaa
     4381 atatcatcac aatggaagat tcaatatgtg gagatgctat tcttataaaa gacaaaataa
     4441 taaaaaagat aggaacaaaa gaagaagtat ttgctttgaa aaataaagat acagaaatta
     4501 tagatttaca aggtaagaca ttgatgccat cttttataga ttcacatagt cacttgatag
     4561 cttttgctac tactctaaag ctagttcctt tagaagatgc aaccagtttt aaagacatag
     4621 taaaaaaaat acaggatttc aaagagtcga ataatataaa aaaaggagac tggataattg
     4681 gattttcata tgataataat tttttagaag aaaataagca tccagataag agtgtactcg
     4741 atagtgcatc ttcagaaaat ccaatactaa tttcacatgc ttctggtcat atgggagttg
     4801 caaatacatt ggggttaaaa cagcttggtg ttaccaatga aacacgtgac ccagaaggtg
     4861 gacatatagg tagagtagaa ggcagtgaag aaccaaatgg atatttagaa gaaaatgcat
     4921 tttttaatgt agcatctaaa ataaaacaac catcatcaaa tgagattttt aattcaattg
     4981 aaaaagcaca gaacatatat ctaagctatg gtattacaac agctcaagaa ggtttaatgg
     5041 aagaaaatca atttaacatt ttaaaagcaa tggcaaatca aaataaatta aaaatggatg
     5101 ttgtaggata tgtaaatttg aaaaaaagta agtctgtagc agataataat agggagttta
     5161 taaaaaaata tataaataga tttaaaatag gaggttataa gattttctta gatggttctc
     5221 cacaaggtaa gacagcatgg ttgagtagac catatgaaaa ttcagatgat ggttattgtg
     5281 gatatcctat atacaaagat gaagaagtag aaaagtttat agatatttca ctaaaagaaa
     5341 agatgcaact cttaactcat tgtaatggag atgcagcagc agaccagttg atagatgcgt
     5401 ttgagaaagt tttaaattta aaagagcaaa gtagtgaaaa tcatataaga ccagttatga
     5461 tacatgctca aacagtaaga gaagaccaga ttgatgatat gaaagtaata aatatgatac
     5521 cttcatactt tgtagctcac acatattatt ggggtgatat tcatatcaaa aaccttggag
     5581 aagatagagc gtttaagatt agcccactta aaacaactat agaaaaggga ttaatttaca
     5641 ctcttcatca ggatacacca gtaatagcac ctaatatgct ggaaacagta tggtgtgctg
     5701 tcaatagaat tacaaaaaaa ggtatacaaa taggagaaaa tgaaaagata agcccattag
     5761 atgctttaaa gggtgttact ataaatgcag catatcaata ttttgaagaa gataaaaaag
     5821 gttcaattaa agaagggaaa ttagctaatt tgataatctt agatgaaaac ccacttacaa
     5881 tcgaccctat gaaaataaaa gatattaaag tattgcagac tataagagaa ggagaagtat
     5941 tgtattcttt gaagtagatg aaagttagat attttataat ctataaaaaa tagttttata
     6001 taataagtta ggtagaatta aataagatta cagttaaatc aacaattata tttttattaa
     6061 attttaatta ttgtattgaa gatttataag tataatttta ttgattctac ctttttaaat
     6121 aactgtcgat ttattaaagt tatctatgta ctatattaaa caattaagta gttattaatt
     6181 gaactta
//
LOCUS       DAYAOX010000214         6178 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.215, whole genome shotgun sequence.
ACCESSION   DAYAOX010000214 DAYAOX010000000
VERSION     DAYAOX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.215"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            176..1777
                     /locus_tag="ACGI57_003148"
     CDS             176..1777
                     /locus_tag="ACGI57_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HFG9718204.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVIQQCISASKSNGQPPAITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSKLEGMANAWSKYMMDNKVFEHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKSVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            1996..3594
                     /locus_tag="ACGI57_003149"
     CDS             1996..3594
                     /locus_tag="ACGI57_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9718205.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVADQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNNLKPNIEGTVDTSKAGTYVLNVSAFDNFGQKCEINVNVKVIINTSTKDPNS
                     YEFKAMVSNEMYNLVNSYRKEKGKKSLRELDSLAGMANAWSKYMDEKKIFAHEIDGKN
                     AAEVFSGFGMRTDENIAYLPMNVKSVYTSKDAKEIAKSVFDLWKKSSKYNDNMLKEEF
                     YSFGFGMYVSNQREVNATMEFLNS"
     gene            3887..6067
                     /gene="slpA"
                     /locus_tag="ACGI57_003150"
     CDS             3887..6067
                     /gene="slpA"
                     /locus_tag="ACGI57_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HFG9718206.1"
                     /translation="MNKKNLAVVMSAVTVIGSAAPVFAAAEDSFPNGNTTVSSSKYSD
                     IERILKKYVADGVTGITVNFFDKKGNPKGVSISSLEASQISYVKDEIKDLKAGEYAKI
                     SVKITDGTEKKYDDTLLKELDSNAVVTKVGTATTVKIVDQDGNSIENDAKLKLQKYDT
                     TTSAYVDGATKDEVLAATKAAVKTSKFTDKTGNNATGTVAITATYADAVAGAIKLKYD
                     TQAAVAPTLDTAVASLDGTKEIYDFSKPVITVNTTDGTSKLSFEKTGEKVGAVESNVT
                     VIAASDETVTISGDAKEKAEALAKKYVFKDTELEDAYKTVTASDFEKTDNDYYEVVLY
                     PTGKRLNTASTYASSNYKPELPTSDRVDTPAIITLRSTNKNNLKSALDELRTYNNSYS
                     NNSVLAGDDRIETAIEISKDSYNADGGIKGDYVEANEVVLVGSQSIVDGLVASPLAAV
                     KDAPLLLTSKDKLDSSVKSEIKRVMGLDDKTGITSKKTVYIAGGENSVSKEVANELKD
                     MGLKVERLSGDDRYATSLEIADEIGLNHNKVFVVGGTGLADAMSIASVASNKEMPIVV
                     VDGKGKDLSTDAKDFIGSAYVDIIGGKSSVSEDMEDAIDDATGKSPERVSGDDRQDTN
                     AEVIKTYFEKDNSDSVISTGVKNFYVAKDGSTKEDQLVDALAIAAVAGHNEAPIVLAT
                     DSLSSDQSVAISKVTNSDDSKKLTQVGKGIADSVIKRIKDLLEL"
BASE COUNT         2486 a          651 c         1214 g         1827 t
ORIGIN      
        1 gaatatgttt aaaaagaagt taaaaataat tttttaaatt taataaaata aaacactaat
       61 atatagacga gatatattca tatcatatat actctataat atatggaaaa taaagagaaa
      121 ataaatggaa aataatcaca atataaatat aaaatataaa aatcatcagg gggatatgaa
      181 aatgaacaaa aaaatattat cattaggtct agcagtatca ttaatcttag taaacttcaa
      241 aagtgtaaat gcatcatcag tagtagaaaa aatatacggt aaagatagat atgaaacagc
      301 agcaaagata gctgataaac agacttatga aacagtaatt ttagtaaata cagaaaaatc
      361 acttgcagat ggattaagtg caagtggatt atcgggagct acaaaagctc caatactatt
      421 tacacaacaa aataagatac cagcagacac aaatagatgt ctaaaaaata tcaaaaaagc
      481 atatataata ggtacagaag atactataag taaatcagta gaaaaagaac tagattctaa
      541 aaatatagaa gtcaaaagaa ttggtggaga agatagacta aaaacaagct atttaatagc
      601 taaagaaata gcaactataa aaaaagttga taaagtacta ctaactaatg catatagtgg
      661 agaagcagat gcaatgagtg tatcatcagt agctactaga gatggagctc caatcatact
      721 tacagatgga aagagtgtgc cttttgatgt aaaaaatatt caatcatatt gtataggttc
      781 agaggagata atgagtaatc ctctagttaa aaatacaaac tcagtaagaa tagaaggaac
      841 tgaccgattt gaaactaaca aaaacgtaat agactacttt tttaatagtg cagatggatt
      901 ctatgtatca gatggatacc aattagtaga tgcaatagca gctgcaccac ttactaaaaa
      961 ctctccaatg gtactagtaa atgatggaag cgataaaact gtattagaag gagctaagaa
     1021 tataacctct gtaggtgaaa tagatgagaa ggtaatacaa caatgtataa gtgcttcaaa
     1081 gtcaaatgga caacccccag caattacagt tggaagtaca gaggtatata agggtgaaaa
     1141 gtttgacact ggcaagttaa atatagtagc taaagataac actgggaagg tattaccaat
     1201 agaagttgat gggtttatag atacaaatag agtaggtaca tacatattga cattaaaagc
     1261 tacagacgaa tggggaaaaa gtacaggaaa aagagtagaa ataaaagtgt tggatgataa
     1321 atctcatgat tacaacagcc cagaatttaa aaaaatggta tctactgaaa tgtataactt
     1381 aatcaattcc tatagaaaag aaaaaggtaa agagcctttg gtagtatcta gtaagctaga
     1441 aggtatggca aatgcatggt ctaagtatat gatggataac aaagtttttg aacattatat
     1501 agatggcaaa aatgctccac aagtattttc tgagtttgga atgagaagtg aggaaaatat
     1561 agcatatatt tatatagatt ctaagagtgt tcaaacaact caagatgcaa aagacttggc
     1621 aaaggctata tttgaagtgt ggaagaaatc tccagaatac aatgcaaaca tgcttagtga
     1681 tgagttttat agtactggat ttgggcttta tatattatct gatggtcaag tgcatgctac
     1741 tcaagagttt ttaaatggca atgaaggtag cttgtaaaaa attccataaa tcttgttttt
     1801 ttgttaattt actgatatta taaaatgaaa atcacatcta aagtactttt tgtaatctgt
     1861 tttattattg ctttacaggt tatggtattt taggtgtttt ttgttttagt agtttataaa
     1921 ttttgaggga atcactactg agggtgtaat ttatgattag ctgaattata taaaaaaatt
     1981 agtaggagga gttcgatgaa ggtaaataag agagtattat caataggatt gacaatatca
     2041 ttaataatgg caggagcacc aaatatcaat gcactttcga gtatagaaaa gatacaaggg
     2101 aaagatagat atgagacttc tgcattgata gcagataaac aaatatatga tacagtcata
     2161 ttagtaaata ctgataattc tatagtagat ggattaagtg caagtggatt atctggagtt
     2221 gtaaaagctc ctataatgtt agtgcaaaga gataagatac caacagatgt tgaaaaaaga
     2281 ctaaaggatg ttaagaatgc ttatgttata ggtacagagg atacaatagg taaatctgta
     2341 gaaaatcaac ttaagaataa agggattgaa gttaaaagaa ttggtgggga agatagaata
     2401 aagactagct atcttatagc taaggagatt tcagctatta aaccagtaaa tgatggtgat
     2461 aaggtttttc ttgtaaatgg atacacaggt gaggctgatg caatgagtgt atcttctgta
     2521 gctgctagag atggagttcc tgttatactt actgatggaa agagtatacc atttaaggtt
     2581 gatggcgttc aatgttattc tcttgggtca gaggagataa tgagtaatga acttgtgagt
     2641 aagactaatt ctgttaggat tgctggtaaa gatagatttg aaactaataa gaaggttata
     2701 caacgctttt ataaaggaac aaagaagttt tatgtttctc aagggtataa gttagttgat
     2761 gctgtggctg gttctccgtt ggctaaggac aaacctatag tacttgttaa tgatggaagt
     2821 gataagagtg tgcttagagg tgctgatgaa gttacttctt taggtggaat ggataagaag
     2881 gttgctgacc aatgtattag ttctgcatct gataaaaata caatgcctac aatcactgct
     2941 aatgatgtag agatttctgt aggggataag tttgataata gtatgttgaa tatagttgcc
     3001 actgattatt atggaaataa tttgaagcct aatattgagg gaactgtgga taccagtaag
     3061 gcaggaacgt atgttttaaa tgtaagtgct tttgataatt ttgggcaaaa gtgtgagatt
     3121 aatgtaaatg tcaaagtaat tataaacaca agtacaaaag accctaatag ttatgagttt
     3181 aaggctatgg tatctaatga aatgtataat ctagttaatt catatagaaa agaaaaaggt
     3241 aagaaaagtt taagagaatt agattcttta gctggaatgg caaatgcatg gtctaagtat
     3301 atggatgaga agaagatatt tgctcatgaa atagatggga aaaatgcagc tgaagtattt
     3361 tctggctttg gaatgagaac tgatgaaaat attgcatatt tacctatgaa tgttaagtct
     3421 gtatatacat ctaaagatgc aaaagagatt gctaagtctg tatttgattt atggaagaag
     3481 tcttctaagt ataatgataa tatgttaaag gaagagtttt atagttttgg ctttggaatg
     3541 tatgtttcta atcaaagaga agttaatgct acaatggaat ttttaaatag ttagagtata
     3601 tattaatatt ttatggtaga aaatattaat atatattgat taaatcagat aaataaatgt
     3661 attaataaaa ttaaaattga aaatttaatt taaaaaatat gaaatagcaa aaaaaagggg
     3721 aaaaatgttg aaagaaaatt ctattaattt tgcataaatt attgaaattc tattttatat
     3781 agtctatcat aagagtgtga gaaatatttg gtttatatga acatggaatt ttaatgtaca
     3841 taattaaaga gatgtaatta atataatgtt gggaggaatt taaggaatga ataagaaaaa
     3901 tttggcagtt gtaatgtctg ccgttacagt aatcggatca gcagcaccag ttttcgctgc
     3961 agcagaggat agttttccaa atggaaatac cactgtatct agtagtaagt atagtgatat
     4021 agaaagaata cttaaaaaat atgttgcaga tggtgtaaca ggcataacag ttaacttttt
     4081 tgacaaaaaa ggaaatccaa aaggggtatc tatatcaagt ttagaagcaa gtcaaataag
     4141 ttatgttaaa gatgaaatta aagatttaaa agctggagaa tatgcaaaaa tatctgtaaa
     4201 aattacagat ggaacagaaa aaaaatatga tgatacatta ttaaaggaac ttgatagtaa
     4261 tgctgtagtt actaaagttg gtactgcaac aacagttaag attgtagatc aagatggaaa
     4321 ttcaatagaa aatgatgcta aattaaagtt acaaaaatat gatacaacta cgtcagctta
     4381 tgtagatgga gctactaaag atgaagtttt agcagctaca aaggcagcag tcaaaacttc
     4441 aaaatttact gataagactg gtaataatgc tacaggaaca gtagctatta cagcaacata
     4501 tgctgatgca gtagctggtg caattaaatt aaaatatgat acacaagcag cagtagcacc
     4561 tactttagat acagcagtag catctcttga tggtactaaa gagatatatg attttagtaa
     4621 accagttata acggttaata ctactgatgg tacatcaaaa ttaagttttg aaaaaactgg
     4681 agaaaaagtt ggagcagttg aatcaaatgt aacagttata gcagcatcag atgaaacagt
     4741 tacaatatct ggagatgcaa aagaaaaagc agaagcttta gctaaaaaat atgtatttaa
     4801 agatactgaa ttagaagatg catataagac agtaacagca agtgattttg aaaaaactga
     4861 caatgattat tatgaggtag ttctttatcc aacaggtaaa agattaaata cagcttcaac
     4921 atatgcatca tctaactata agccagaatt acctacaagt gatagagtag atactcctgc
     4981 tataataaca ttaagatcta ctaataagaa taacttaaaa tcagctttag atgaattaag
     5041 aacttataat aattcttatt caaataattc tgtattagct ggtgatgata gaatagagac
     5101 tgcaatagaa ataagtaaag actcttataa tgcagatgga ggaattaaag gagattatgt
     5161 agaagctaat gaggtagtat tagtaggatc acaatcaata gtagatggtc tagtagcttc
     5221 acctttagct gctgttaaag atgctccatt attattaact tcaaaagata agttagattc
     5281 atcagtaaaa tctgaaataa agagagtaat gggattagat gataaaactg gtataacatc
     5341 taaaaagact gtttatatag ctggtgggga aaattctgta tctaaagaag tagcaaatga
     5401 attaaaagat atgggattaa aagttgaaag attatctggt gatgatagat atgctacatc
     5461 tttagaaatt gctgatgaaa taggtttaaa ccataataaa gtatttgtag ttggtggtac
     5521 tggattagca gatgcaatga gtatagcatc agttgcttct aataaagaaa tgccaatagt
     5581 agtagttgat ggaaaaggaa aagatttatc aactgatgca aaagacttta taggaagtgc
     5641 ttatgtagat ataataggtg gaaaatctag tgtttctgaa gacatggaag atgctataga
     5701 tgatgctaca ggtaagagtc cagaaagagt tagtggagat gatagacaag atactaatgc
     5761 agaagttata aaaacttatt ttgaaaaaga taatagtgac agtgtaatta gcacaggtgt
     5821 taaaaacttc tatgttgcta aagatggttc tacaaaagaa gatcaattag tagatgcgtt
     5881 agcaatagca gcagtagcag gtcacaatga agctccaata gttttagcaa ctgatagttt
     5941 atcttctgac caatcagtag caataagtaa agttacaaat tctgatgatt ctaagaaatt
     6001 gactcaagtt ggtaaaggaa tagcagattc agttattaag agaataaaag atttattaga
     6061 attataagat aaaattaaac tataaataag agggaacttc tttgaagaag ttctcttttt
     6121 tataaaaaga aattaaaaaa ggaactaaaa atagttcctt tttttgtgag aaatacct
//
LOCUS       DAYAOX010000215         6111 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.216, whole genome shotgun sequence.
ACCESSION   DAYAOX010000215 DAYAOX010000000
VERSION     DAYAOX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.216"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            305..1117
                     /gene="modA"
                     /locus_tag="ACGI57_003151"
     CDS             305..1117
                     /gene="modA"
                     /locus_tag="ACGI57_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9718207.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            1127..1918
                     /locus_tag="ACGI57_003152"
     CDS             1127..1918
                     /locus_tag="ACGI57_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718208.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAIILSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILYTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            1920..2615
                     /locus_tag="ACGI57_003153"
     CDS             1920..2615
                     /locus_tag="ACGI57_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9718209.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2602..3333
                     /locus_tag="ACGI57_003154"
     CDS             2602..3333
                     /locus_tag="ACGI57_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HFG9718210.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEICAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3647..4447
                     /locus_tag="ACGI57_003155"
     CDS             3647..4447
                     /locus_tag="ACGI57_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFG9718211.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            4987..5703
                     /locus_tag="ACGI57_003156"
     CDS             4987..5703
                     /locus_tag="ACGI57_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9718212.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            5672..>6111
                     /locus_tag="ACGI57_003157"
     CDS             5672..>6111
                     /locus_tag="ACGI57_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9718213.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INYYYKEFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLI"
BASE COUNT         2393 a          730 c          852 g         2136 t
ORIGIN      
        1 ctatatgttt caatgttcta ttttttcact gaaatataat aaatataaaa ttagttaaaa
       61 agtaaaaaat ctacaataag ttaatttgat ttaagttttt aaatttaaaa tactttaaag
      121 tattaataaa aattaaaaat aaaataacta cctatagaaa taacattata aatagatttt
      181 ttcttaacta tacataacaa aacataacaa aacattataa aacataacaa aaacttagac
      241 atcgttttct tagtatgata tactaaaaaa gaatttacat taatttacta agggggaata
      301 tcttatgttt aaaaaacttt cagtactagc tttagctatg attatttcat taagtattac
      361 tgcatgtaat tctaaagaaa acaaagctaa tgatgaatct aatactaaag tgacaacagc
      421 tgatgattca aaagaaaaag agactctttt tgtctattca ggggcaggtt taaagaaacc
      481 aatggatgaa atagcaaaaa aatttgagaa agaaaataat gtcaaagttg aatatagcta
      541 tgcaggttct gcacagctaa tagctcaaat tgagacaagt cacaaagggg acgtatttat
      601 tgttggttct gaaccaattt attcaaaagc ctttgaaaaa gaacttgtag gtgaacataa
      661 aacggttgca catcatacac ctgcaatagt tgtacctaaa ggaaatccag ctaatattca
      721 aaaattggaa gacttaaata aagatggagt taaacttatt ttgggagaca aagagtctaa
      781 tgctataggt aagactacac aaaaaatact tgaaaaaaat aatttaaaat ctataaatga
      841 taatgttgtc tctacagctg caactgtaaa tgaaatgatt gtacagttaa catcaagtaa
      901 agctgatgct acaatagcca caaaagattc ggtctttggc aatgaagatg tagaagttat
      961 agaaattgcc caagataaaa atatagacca acttatatca gcaggaatcg taaactatag
     1021 tgataaaaaa gatttagcta ataaatttat aaattatata gagtcagacg aagctaaaca
     1081 gatatttaaa aaatatggtt ttgaacctgt taagtagtga ttaaacttga aaaataaatt
     1141 ttttactact ctacttactg gattttttgc tatagtcttt ttgtttattg tatcaccact
     1201 tcttatgtta atcattaaag ggataagtta tgttcctata tgcttaaaat cagtggaagt
     1261 acagtatgca ataatactta gtattaaaac atctttaata tctactatag tatgcctttt
     1321 attagctatt ccagtagcat attttttaca tattactaaa ttaccattta aaaaactgat
     1381 tatacagata ataaatctcc ctatgtctct tccacactta gtatcaggaa ttgctctttt
     1441 acttttgttt ggaagaatgg ggattggaga ttccatttat aagattttta agcttgattt
     1501 tatatttaca aagcaaggta tagttttagc acaagtattt gtaaatcttc cacttactat
     1561 taaaatactt tacacttcac taaatgaaag taatgaaaag atgatatttg ttgcacgtac
     1621 ccttggatgt aatagttggg aagcatttag atttataatt cttccaaatc taaaaactgg
     1681 aatcatatct gcaactgtaa tgacttggtc aagagcactt ggtgaatttg gtgcagtagc
     1741 tatgatagca ggttctacac gtatgaaaac tgaaattatt cctacatcta tatatttaaa
     1801 tatgtctact ggtgatatag acattgcaat cggaatagct gtaattttga tatttatctc
     1861 acttacatgc ttgatgcttt ttgagatatt ttttaataga gaggttgata agaattaata
     1921 tgttaaaaat aaaaaattta tctcttgatt tacctaattt taaattacaa gaaaatagtt
     1981 taaatattaa taatcatgaa tactttgttt tattaggagc tagtgggtca ggtaaaactc
     2041 tatttctaga aacattagca ggaagatatc catctgctaa aggaaatgta tcatacaaag
     2101 aaaatttaat atcaaactta cctccagaaa atagaaatat aggatttgta tatcaaaatt
     2161 ttgaattatt tccaaatatg aatgttgaag ataatataag gtttccactt aaattgagaa
     2221 aaatacctat agatgaacag attcttaagg ctaaccactt atctaaatta atgcagatag
     2281 aagatataaa aaataggtat cctaaaaatt taagtggagg tgaaaagcaa cgagttgctt
     2341 ttgcacgtgc cttaatagct gaacctgata tattactttt agatgaacct atgagttcat
     2401 tggattatat tacaaaaaaa catataagca aaattttgaa ggatgtctac ataaaataca
     2461 aaccaacagt gattcatgtt actcatgata tttctgaagc aatgtttttt gctcataaga
     2521 taggaataat gaacaatgga aaaataaatc atatttttga tttaaatgaa gaaataaaaa
     2581 ataaaggaga atcttttttc tatgaatata tttgatattt tgagagatta tctattagta
     2641 caagttgata aatataattt aaatatggat atgataagta ttgtgtccaa atctctatct
     2701 tcaaaagaag caattggaaa tactaagcgt aaagatttcc caattatagt tggtaaagaa
     2761 ataatgcttg aagctgactt taaaggagct aaaggacaag catttacttc tactccatct
     2821 acatttgaag gttctttaaa agacatatta tcattagatt tacatgacaa tcctcatgat
     2881 agaagtcttt ttatagcatc attaaatgca gttatgaaat acttaggcaa gacggataga
     2941 accattcatt gtaaaaataa cgagccagag atatgtgcaa aaaaattccc tgagtttata
     3001 aaaatggaat ttggtaatcc aaaagtagca attataggat atcaaccagc cataattgat
     3061 aatattaagg actttttcga aacaagagta ttagatttaa atcctgaatt tgtggatact
     3121 atccaatata atgttaagat tgaagatggt atacgagatt atgaagatgt aatatcttgg
     3181 gctgatttag tgatttgcac aggaagtaca ttatgtaata actcaattat aaattttcta
     3241 tcacttaaca aaccagttta ctattatgga actacgatag ctggtgcatc aaatatttta
     3301 ggtttaaaaa gactctgttt ttgtagtaaa taagcaatca tatcatcaag gagtacacaa
     3361 atatatatgt gtactcttta aatttaaaaa gttattactt ttattctaag tactaaagta
     3421 aaattctatt aaaatgaatc ccgaataaat gtacaatccc tttaaatctc aattcctatc
     3481 aatcattaat attttgatta gtcttataca taaatgtttt ctttatttta aactaataaa
     3541 aaaattttat tccattaatc tactctatat ttttcagcat ttaagaaata atttttagta
     3601 ctttgtttga taatataact aagacaactt ttcaggagat gattaaatga catttaagaa
     3661 taaaaaattt actataaata aatataaaat tctatctatt attctagttt tatgtttgat
     3721 tttaaccgct tttagtagct atgtgaatta tagtcataaa aaatttaaag attatgttgt
     3781 cttctatgtt cagcatcaag atgatgaagt gttgtgggct gggagtgcta ttgttaatgc
     3841 tataaaagaa agaggaaaaa atcatgtatt tgtggtttta gtatctactg gatgtggtat
     3901 atccgtcttt gataaatata aaaaatatga aaacctaaca aatatacaaa aatcagaata
     3961 tagaaataga gaatttttag cttctcttaa tagccttggt gtaataaggg aaaatataat
     4021 attattacca gaaacaaata caaatggtaa tactgacttc aattacatgg aaaaaattgc
     4081 tcttacattt gaagaaaaat ttaaaaatgt aactcatata gcacatactt ataagttaga
     4141 tgaccattta caacatttaa aaaatggttc tgttatccaa aatttatata atgctggtaa
     4201 aataaaagat gctaaatatt ttgtaaaacc taaatttgct gataaaataa cttttaataa
     4261 taagatagtt tataaagctc attctactga agactatgaa aaggttaaaa atgcctgtca
     4321 acaatacaaa attgttgatg aattagaata tagagaaggt attggctata agtctgacca
     4381 taaatcattt gataagttat taaataataa agatgttcct gctatacttc atacacctaa
     4441 tttataagaa atatttattc ttttaattta agttataaaa aggaaattat ttgagatttt
     4501 gtgatatagc actttttgtg ctatactact ttttctcata caactattac ccatatctaa
     4561 tttcatcatc tcttaaaata tataaagata tcatcattaa aacctataag ccttattaaa
     4621 tgctttatac atatctacat taagctacct atttatatta aactacatta aaataaatat
     4681 ttgttataaa ttcttaataa ttatgaaact catttgttat ttatatttgt taatatatgt
     4741 ttaaacctaa aaaattaata aaccaaaata acataaggag tgtgatagat taccctagat
     4801 attttcataa acttttacct tcagattaat atgggcgtct atacttttaa atttactata
     4861 tgcattgaca atatattaaa agtactagtt tttatttcat aatgaacggg cttactgttt
     4921 tctaatacct ctttttggta taaaataaaa attggttaaa aataaaattt caagggggtt
     4981 aataagttga atcaaaatat attggttgta gatgatgata gagagatagt tgatgctata
     5041 gagttctatt tgagacctga taactttaat gttttaaagg catatgatgg acttgaagca
     5101 ttggacatac taatcgaaaa tgatattaag ttaataataa tggatgtaat gatgcccaat
     5161 ttagatgggc ttaaaactac acttaaaata agagaaaaaa ggaatatacc aataattctc
     5221 ttatctgcaa aatcagaaga tatggacaaa attataggtc ttaatatggg tgctgatgac
     5281 tatataacta aaccatttaa tccactagaa cttactgctc gagtaaaatc tcaacttaga
     5341 aggtatataa atttaggtga ttttagtaat acaaataact caaatgatac taattatatt
     5401 ctaaaaagtg gtggcctaga gctaaataca gatacaaaaa tagtttcttt agatggtgaa
     5461 gaaattaaaa tgacaccact tgaatataaa atacttacac ttcttatgtc acgaaaaggt
     5521 aaaattttct cttctcatga gatttatgaa aatgtctgga atgaggatgc ttatagttgt
     5581 gagagaacta ttgctgttca tattagaaga attagagaaa agattgaaat taatccaaaa
     5641 gaaccaaaat atttaaaggt ggtgtggggc attggttaca aaattgaaaa acttaattat
     5701 taatgattct aaagcaaaat ttattttatt attattactg tttctattag tttctatatt
     5761 atttgaagca gtttatgatt tgaataataa cttttcttat aatattaatt attattataa
     5821 agagtttgct ctcccttatt tttatttttt cggtactcct gtacatttgt caaaagtaga
     5881 attagagttt gtctgcatag gtattatatt atcaagtttt ttattgacta tactatgttt
     5941 tacattgtat aaaaaaaata aacagactaa agtcaaattc ttagagaatg tcataagcct
     6001 tttttctaag gtaaaaatag aacttacact tatattaact ttgatactat taaccttaag
     6061 gtttcatgca tttacaagta ataaaatgtt ttacaacact aatttaataa t
//
LOCUS       DAYAOX010000216         6049 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.217, whole genome shotgun sequence.
ACCESSION   DAYAOX010000216 DAYAOX010000000
VERSION     DAYAOX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.217"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            113..733
                     /locus_tag="ACGI57_003158"
     CDS             113..733
                     /locus_tag="ACGI57_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFG9718214.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(899..1468)
                     /locus_tag="ACGI57_003159"
     CDS             complement(899..1468)
                     /locus_tag="ACGI57_003159"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFG9718215.1"
                     /translation="MFIVTIQEDFYMIKETHERGGYIFALLILPFINNLYLVKYDIAY
                     KIVLIIIYIYFAYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLI
                     YSFFKSLTIFTNNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIK
                     TNSKTEKFISKLLNFIVIFLIGYQFYILL"
     gene            complement(1548..2252)
                     /locus_tag="ACGI57_003160"
     CDS             complement(1548..2252)
                     /locus_tag="ACGI57_003160"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFG9718216.1"
                     /translation="MKKKILIPVIMSLFIISQCITSFAFTPENNKFKVKPLPYAYDAL
                     EPYIDKETMKLHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVR
                     NNAGGAYNHKFFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWA
                     WLVATKDGKLSIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVV
                     NWNGALENYKNLKSQD"
     gene            2425..4299
                     /gene="asnB"
                     /locus_tag="ACGI57_003161"
     CDS             2425..4299
                     /gene="asnB"
                     /locus_tag="ACGI57_003161"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFG9718217.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(4391..5827)
                     /locus_tag="ACGI57_003162"
     CDS             complement(4391..5827)
                     /locus_tag="ACGI57_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFG9718218.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
BASE COUNT         2327 a          704 c          884 g         2134 t
ORIGIN      
        1 aacttatata gattatttac tttttaatat tagattcatt ttttgagatt ggtatataat
       61 aaacatatag agtatataat aatagttaaa gttaaaaaag gggaaaagca atatgcagta
      121 tatacaagaa ctacttgatt tgactcaaaa ttattgggtg ttagccacaa taatcggctt
      181 attaagtgca tttatagaaa gctttatacc agccttacca cttgttgcta tagttacagc
      241 aaatgctgca atacaaggat tatttattgg atgtttgtta tcatggattg gctcaggact
      301 tgggacaact tcattattct tattaataag cagatttaat gatagtaagc tttttaataa
      361 gttgagaaat agtaaaactg aaaaagcaat aagttggatg gataaacagg gatttaagtt
      421 attatttata gcgtatgcct gtccatttat gcccggttgt ttagttacta tcgcatcagc
      481 ttttggtaaa aaagatttaa aagattttgt gccagcaatg ttggcaggaa aatttgtaat
      541 gtttatagtt ataagttacg tggcaagtga tatagaaggt tttataacaa gtcctttaaa
      601 gatagcatca tttatattat tggtgttttt gtcatggaag attggaaata gagtaaataa
      661 aaatcttgaa aatcataatt atgatttcca tcataaaaaa catcacaatg ataaagatga
      721 taaaatgatt tagcttgtac gaaataaaac gtatcttaaa atgatttagt actaaattat
      781 tttaagatac gttttatttt ttgtattaag gtacaaaatt tttagttctt ttgtaaacta
      841 aaatatataa aacttattta tacttagttc aaaatattgt tatttatttt ttctacaact
      901 ataataaaat ataaaattga tatccaatca aaaaaataac tatgaaattt aatagcttag
      961 atatgaattt ttctgttttt gagttagttt ttataggtag taaagaaatg tttatggtaa
     1021 tgggataaaa caattcaata ccttctttag taataaggtc aagacacaag tgagatagat
     1081 atcctattat aaacccactg gacaaacatg agaaaactat attattgtta gtgaagattg
     1141 tcagagattt aaaaaaacta taaataagta ataaggcaat tagactgtga gtaaaccctc
     1201 tatgtctaaa cctacttcca aataatttgt atattaacat aaatttttta cttatgtaag
     1261 aacctctcat atctatatct ggaaataaag aacctaaata tgcaaaataa atataaatta
     1321 taattaatac tattttatat gcaatatcgt atttaactag gtatagattg tttataaagg
     1381 ggagtatcaa aagtgcgaat atataacctc ccctttcatg tgtttctttt atcatataaa
     1441 aatcctcctg aatcgtcact ataaacatta tattggtaaa tttagactat cttttattat
     1501 attatatagc aaacataagt ttttttcaaa aggattttaa tatttaatta atcttgagat
     1561 tttaaatttt tgtaattttc taaagcacca ttccaattta ctacattaaa ccagttgtca
     1621 atgtattcat ttcttctatt ttgatatttt aaatagtaag catgctccca aacatcaagt
     1681 cctattatag gagttaggtt tttacttaca gggctatcct gatttggagt agtcataata
     1741 gataatttgc catctttagt agctacaagc caagcccaac cagaaccaaa gacatctaaa
     1801 gcagattttt ggaactcttg cttaaatttt tcaaaagaac caaagtctct atcaatagct
     1861 tcttttaaag attcagaagg tatggttttt tctggcgtca ttatatcaaa aaagaattta
     1921 tgattataag ctccacctgc attatttctt acagttgtag caatatcttt aggtaaagaa
     1981 tctaaatttt gcaataattc acataaagaa taattataaa gctcaggata tttttcaaga
     2041 gcagcattta atttatcaac ataagcttga taatgcttat catgatgcag tttcattgtt
     2101 tctttatcta tataaggttc aagtgcatca tatgcataag gtaatggttt aaccttaaat
     2161 ttgttatttt caggtgtaaa agcaaatgaa gttatgcact gtgagattat aaataaagac
     2221 ataataactg gtattaatat ttttttcttc ataaaaatca cctcctaata tagtatttct
     2281 agaaataaaa aaaatatgaa atattaacag ataatttata agaatttaat agcaatttca
     2341 ttttaaattg aataagtttt aaagccaaag acaaaactat gaaatataga ataagactaa
     2401 aggacagaaa ggaattttga ttatatggac ggcaatattt caatttatta taaaggtgat
     2461 atatctaaaa aagaagatat taaatttaaa ataaaagaat ctgtaaataa tacaagactt
     2521 tctagtagaa tagagtataa ggatgaggaa aaaacttttg attttgaaag atttaatgta
     2581 attgatgcta tttatggaaa taaaccattt gaaaaagatg gaaaagttat tgtttttaat
     2641 ggaaatatat ataactatca tgaaataaaa gaagaattaa ttaaaaaagg acacaatttt
     2701 actacaaatg atgatattga agtcttatta gcatcttata tagaatttgg taagaattgt
     2761 gtacataaga ttaaaggaat gttcaacttt ataatctatg atagagaaaa tgaaagtata
     2821 tttggagcaa gagatttatt tgggataaaa ccactttatt atataaataa ggaaaatgca
     2881 ataatttttt cctcagagta taagttttta cttgaatata tgaaaaattt aaatataaat
     2941 gaaagaagct tacaaagtta tttttcattt caatatgtac ttcctgaaga tactatgata
     3001 caaggaataa ggctgatacc agcaggtcac tattttaggg tagaaaatgg tatactatct
     3061 ttaaagagat ataataaact tgagtttaga agtagtacaa aattttttta tactaaaaat
     3121 cacttaggca atagagatgt gaatgaggat gatataagaa atatagtagt agattcaata
     3181 gtaactcata tggaagagga gaaagaaatt ggaacatttt tgtctggtgg aatagattca
     3241 tctattataa ctactgtggc ttcacaaatt aatccaaata taaaatcttt ttctgcaggc
     3301 tttagtgtca aaggatatag tgaattagaa gtagcaaaga aaacagctga taagttaggt
     3361 atagaaaata tccagattaa tataacacaa gatgaatata taaaatcatt accgaatgta
     3421 atttattctt tggatgaccc tatagcagac ccttcagaag taggtcttta tttcttaatt
     3481 aaagaggcag gtaaacatgt taaagtagca ttgtctggag aaggagcaga tgaattattt
     3541 ggaggatata atatatataa agagtatagt actatgaaat cagttgtcaa ttcacctact
     3601 tatattaaaa gtatattagg aagagtttca gaacttatgc caaacataaa aggaagaaat
     3661 tatctttata gagctacaac accattagaa aaaagatata taggaaatgc aaaagttttt
     3721 gaaaacagcg aagtaaaaag attctttttt aagtataagg aaaagaatat atatgaatac
     3781 ctgttgtcta atttgtatag agatgcacaa aaaaataatt acgattatat aagtaaaatg
     3841 caacatatag atgtgaatac atggcttcaa ggcgatatac ttcaaaaaat tagcaaactt
     3901 tctacagctg agcaggttga acttagagta ccatttttat ataaagatgt atttgatgtc
     3961 gcaaaaaatt taagaatgga gcaaaaaata aataagaata atacaaaagt tttacttaga
     4021 gaggcgttta gagaaatagt cccagaacat gttgttcaaa ggaaaaaatt aggatttcca
     4081 actccaataa gagtttggtt aaaagattct ctaggggata ttgtaaagga aactatttat
     4141 aattcaaatg tagatgaatt tatagataaa aaatatgcaa taaaactttt agatacgcat
     4201 cttaaaggtc atagagataa ttcgagaaaa atatggacta tatttacatt ttgtctatgg
     4261 catcaattat ttattgagca taaaaatgtc gagtattaat acttttttca atattaatca
     4321 gagggcaaaa gcatgcaatg ttttcaccct cttgattatt atggaatagg ataagtagaa
     4381 ttttgattga ttagtttact atctcaataa aattattggc aaatattttg acaagactac
     4441 tttcaacagc taaatttaag ttttgtccat catcatatcc agcggaaatc aatcctagta
     4501 agtttccaaa catatctaaa agagctcctc ctgaactacc actagagatt ggagctgtaa
     4561 attgtatcat ttgaaccgtt tcaaaatcac gaaaagctga aataattcca tctgaaactg
     4621 tattaaaaag acctagaggg cttccaattg caactatttt ttgacctctc acaggttttt
     4681 tactgacttt aacaggtatt ggcttgcatt ttctatcaac ctttatcaca gctaaatcat
     4741 aatctgaatg gtattttata atttggtaag atgtataaac tttatcatca ttttcaaatc
     4801 taactaaaaa tgaataccca tcattgacta catgaagatt cgtaagtata taaccttcat
     4861 tatttattac tactccagaa ccgctcttaa acggtttatt ttcgttattg cacacaacaa
     4921 tcataacaac agaagctgat aactttgcaa ggtcttcata agaaagttct acttcctcat
     4981 cttgtacaat ttttttagat gttagatttg tgttattaag tgtattttta gagttaggtt
     5041 tattcgtagt aggagaagtc ttattggtat tttcactgta atttatattt gtaatatgcc
     5101 tttcaaaagg tatttctaat atatttggag tcttcctaga aagttcaaat agagtagaaa
     5161 attttgaaca ttcaggatat aaatcaagaa tatctttatt tggatttata tataaaatat
     5221 cacacccaag ttgtgataaa aaatataaga aaaaaagttc ttgcctttta atatttccaa
     5281 agtatactat ctttggtgaa atagtagtct tcaccttgtt ataaaataat ttaggtagaa
     5341 aatagtctat ccaacacaaa agtttaactg caaagttttt gatgatagag gatgtacaat
     5401 ttggcatact tgtggaaaat aaatctaaag tatttttaaa tgcaatcttt ttagtccaat
     5461 ttaaggtgtc attttctatt ttacatggaa agtcgatatt atataaatcc caactgttaa
     5521 acttagaact attataaata ttaaaaattt taagcatctt atctgtttgt tctttgtcaa
     5581 aatttatttt tagattgttt aatctaatat agggaattgt aggattatta aaaaaataag
     5641 tatctatatt tttaattttt tctatgtatt tatcaattga atcatcaaca cctatatatc
     5701 ttgtaaagta gacttcttta atatatgaag aactcttatt tttataggta gtatcagata
     5761 taaattcaat tagaggttca gtagactttt ttattgaaat ctttaatggt ttaatagtac
     5821 aaatcatatt ttttctccta taataagatg atataatcta ttataaaaca acttaaaaca
     5881 atatagtaca ataattttgt agtttcaaaa taaaatatgt acttgtttat ataaatgata
     5941 tataattaca ataaaattgc cgaaaagccc tctcctttag gtgtagggat gaaaggtaag
     6001 gtcttaatat aaaaaaagtt agaataatgc ttgtaatcta ataccgcta
//
LOCUS       DAYAOX010000217         6036 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.218, whole genome shotgun sequence.
ACCESSION   DAYAOX010000217 DAYAOX010000000
VERSION     DAYAOX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.218"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(106..1320)
                     /locus_tag="ACGI57_003163"
     CDS             complement(106..1320)
                     /locus_tag="ACGI57_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9718219.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            1564..2946
                     /locus_tag="ACGI57_003164"
     CDS             1564..2946
                     /locus_tag="ACGI57_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HFG9718220.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIESGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            2958..4307
                     /locus_tag="ACGI57_003165"
     CDS             2958..4307
                     /locus_tag="ACGI57_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HFG9718221.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSITSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            4457..4990
                     /locus_tag="ACGI57_003166"
     CDS             4457..4990
                     /locus_tag="ACGI57_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFG9718222.1"
                     /translation="MSGAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            5074..5883
                     /locus_tag="ACGI57_003167"
     CDS             5074..5883
                     /locus_tag="ACGI57_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9718223.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
BASE COUNT         2227 a          691 c         1052 g         2066 t
ORIGIN      
        1 ttataaataa agtattttta aaaggaacat tataaaagac tgtatcaaag ttaactttta
       61 gaattataaa gtttcatctt tttacagtct tttaatatat aatatctatt tatcaaatct
      121 atctacttta aataatttca tatttttatt ttgtttgcca agatataatt cacttaacat
      181 tatacctcct aatattgcaa agagtatacc atttgctcca taacctaagc aataccatag
      241 attgttattt ttattatctg gaccaataaa acctaaatta tctaaagttg aagcaaaaca
      301 accacaatat ttatattcaa cttcaatgtc ttttatatcc ttaaacatac ttttcaatct
      361 ttgttctaaa atatcatact tttcattggc taaagtttca ttttctatat catcaaaact
      421 tacatcttca ccacctataa ttaatcgatt gtctttagta gttcttagat aattatatgg
      481 gtcacagtta tctctaatta aaatgttgtt tttccaagaa gctatgtttt taagtggttt
      541 cgttgctata ttaaaagtat ttgacttagt tgcaaaattt ctttttgtaa ataaacttgt
      601 attgtaccct gtagctacaa ttaatttttt accatgaact ttgtatccat acgatacttc
      661 agcagtaacc ttatcatttg ataaatctac tttttttact tctgtatttt catatacttg
      721 aagaccattt tttaaactta catcaatcaa atggtgacta tatttatatg ggtctaattc
      781 tcgtcctcca ttctttgcta tcaatcctga ttttaaatca aagctaaaag ggtttgtgga
      841 ttcatcaata tattcaacat caaatccatt ttcttttctt aagttatatt cttctttgat
      901 agccttaact tcaagtttgt tggcagtata aagtagagta tctcttttgg cataatcaca
      961 cttatttcca tatagctcta taaatgtatc taattcttca agtgctgaag tacaaaggtt
     1021 gtatgctttt agagcatctt ttagagtcat aacctctgtt aaatctatca aattgtcatc
     1081 taattcatac tgtaatagag aagttgtcac gctagtgctt ccatgtgcaa tccttccttt
     1141 ttcaagaatg acagacttta tattgttttt agctagatag taagcacaaa tacatccagt
     1201 tacgccacca ccaacaatta taacatcagt atcaatatca tcagtaaggt atggaaattg
     1261 tcttggaact ttattaatat tagtaaaaat aggatttcct tttacatatt cttgattcat
     1321 atacttcact cctaaaattt attatgtatg agtgtattat tttctatagc aaataatatt
     1381 atacatttta attatctagt tatatgggta tgtatagata aaatgtgata acattttagt
     1441 ataaaacata tgtataatat attcataatt accttatttt aagtaatata gtatataatg
     1501 tttgttaaca agtaaaaaga ttgaataaaa gttaattaaa aatttaaagt aggaggtaaa
     1561 tttatgcttt caaaaaatat agcttctaag gtattagaaa aatgtctaat tactggagga
     1621 gattttgctg agatttttga agaagacact ataaataatt ctattagttt aatagataat
     1681 aaagtagaaa atgcaatagg tggaagaaat tatggtatag gaataagaat atttaaagga
     1741 ttgaaaagta tatatgctta tacaagtgac aattcattaa atagtctgtt ggatgtagca
     1801 tacaaagctg ctgttgcttt aggtaaattg gaagatggaa aaagtgtaat attaaatgac
     1861 agtattaaaa taaataacat acataatata aaaatttatc caaattcaat aggaaataaa
     1921 gataaagtaa gtgttatgaa ggttgcatat aagagtgcaa aagaatttag taatgatata
     1981 tctcaagtaa gtgtcagtta tttggataaa gaccaaaaaa tattaattgc aaacactgaa
     2041 ggtatatatg ttgaggatag aagaataaga actagactag ggataagttc aattgcatct
     2101 aagggtaatg aaaatcaaac tggatttgaa ggtcctggag gttgtaaagg gtttgaaata
     2161 tttgaagaaa tagacccaga atattatgca aaagaatcag ctagagtagc gcatactatg
     2221 ttgcatgcta aaaattgtcc tgcaggtaat atgatggtag caatagataa tggatttggt
     2281 ggagtaatat ttcatgaagc ttgtggacat tctttggaag ctacatcagt tgcaaagggg
     2341 aactctgtat ttgcagataa attaggtcaa cagattgctt ccacaaagat tacagctata
     2401 gatgatggta caataccaaa ctactggggt tctatgaata tagatgatga gggaaatcca
     2461 actcaaaaga atatactgat tgaaagtgga attttgaagt cttatatgat agataaactt
     2521 aatggtagac gaatgggaat gaatcctaca ggaagttctc gtagacaaaa ctacaaattt
     2581 gcaccaactt caaggatgac taacacttat attgctgctg gagaagatag accagaagat
     2641 attataaagt caataccaga tggactttat gctaaaaaaa tgggtggtgg ttctgtaaat
     2701 cctgtaacag gagagtttaa ttttgcagtt tcagaaggtt acttaataaa aaatggagaa
     2761 atacaagagc cagttagagg tgctagttta attggaaaag gtagcgatat acttatgaat
     2821 atagatatgg ttgggaataa cgtaaaacaa gctcagggga tgtgtggctc atcatcagga
     2881 agtataccaa ctaatgtagg ccaacctatg ataagggtaa aagaaattac tgtaggtgga
     2941 agataggagg gataattgtg gaacttaaaa attttaaaga tatattattt aaaaaggctc
     3001 taagtgaagg ttttgaagag tgtgaaatat attattatac aggagaaaat ttaagtataa
     3061 atatatatga aggagaagtt gagaaataca atcttgataa atcttttggt ttatctttta
     3121 gaggtaaggt aaatggaaag ataggttatt cttatacaga aattttagat gataaagctg
     3181 ttgatatgtt gataaaaaat gtaaaagatg gagtaaacac tattgaaaat gaagatgtac
     3241 aatttatata tgaaggagat aagcactata atgatgtaaa gacttattct aaagagctag
     3301 aaaatttaga ggcagataag ttgattgatt tagctttaga aatggaaaga gagactaaag
     3361 cttattcaga taaggtggta aatttatcta aatgtactat atcttattct gtatcaagta
     3421 atggtatatc taatactaaa ggtcttaatt taagcaataa aactaatatg cttatagggt
     3481 ttgtagttcc tatcatagaa gacaatggac aaaaatatga tggaatagga tataatatag
     3541 caaattctat tgaagaaata aaaccttgtg agatagcaaa acttggtgtt gataatgctt
     3601 tatctaaagt aggtggtaag agtattacat caggtaaata taaaactata ttgcttaatg
     3661 aagctatggt atctttgctt agcacatttt ctgggatatt taatgcagat tcagctcaaa
     3721 aaggattatc tttattgaaa aatagagaag gagacatgat agcatctcct atagttacaa
     3781 tagttgatga cccactttta gaaaatggta tggcatctac tccttttgat gatgaaggag
     3841 tagctacatt taaaaaagaa gttgttttaa atggaaagct tataacttta cttcataact
     3901 taaaaacggc taataaggca ggtgtaaaga ctacaggaaa tggaataaaa tcttcatatt
     3961 catcacctat aagcatatat ccaactaatt tttatataga gaaaggagat aaatctttag
     4021 atgaaattat aaaggatata gatgaaggat tgatggttac tagctttgca ggacttcatt
     4081 caggtgcaaa ttcagtaaca ggagactttt ctttagctgc caagggcttt tatatcaaag
     4141 aaggaaaaaa agtatttcca gtggagcaaa ttacagtagc aggaaactat tttgacctgt
     4201 tgaaagatat tgaagctata ggagaagatt tagaatttcc aatgagtagt ataggttcac
     4261 cttcagttgt tattaaagag ctatcagtag ctggaaaaga tgaataatta aaaagtaatt
     4321 ttgtggataa aaggtcatgt taattttaat taatatggct ttttattatt taagtagatt
     4381 ttattatcta agttatatga aaaagttgtg atgcatacaa ttaatatcat acattaaatt
     4441 taaaggagga gatattatga gtggtgcaca ttatgacatg cacaagcgaa atggatattc
     4501 aagtgatgag ccatatccag agataaaagt tttaggtcca aataagtatt atgcagaatt
     4561 attgatggat gactatgctg gagtatcaag tgaatttacg agtgtaaatc aatatctata
     4621 ccataatttt gatttagatg aaacgcatag agagttaagt gaaatgtgga taaatatatc
     4681 tataacagaa atgttacata tggagatatt agcaaagact ataagattat tgggtggaaa
     4741 tcctgtatat agaggttcta caagttcttg tggtgcatat tggaatggtg gttttgtatg
     4801 ttatggaaat tctatttgta acagattaaa actagatttg catttggagc atgtggctat
     4861 taataattat tataaagata taagtcttat tgaagaccct tatataaaag ctatattaaa
     4921 tagaataatc ctagatgaaa aacttcatgt aagtttattt gaaaaggcaa tagagaagta
     4981 ttgtaaataa atttgaaaat ttagttattc atcattttat tcatatatat aaaataacca
     5041 tcatacattt tcttgaaggg agttgctgtt gatatgtatt atgtatatgt gaatgatata
     5101 aacatagcag tatatgattt gaatccgagt gctaaaaaaa ctgtactttt tatacatggt
     5161 tggcccttag gacataagat atttgaatat caaacaaata tattgccaaa actcggatat
     5221 agaacagttt caattgattt aagaggtttt ggtaaatctg atgcaacatc aggaggatat
     5281 acttatagcc aattagcaga cgatatttat aaggtggtac atgctattgg attaaaagac
     5341 tttacccttg ttggtttttc aatgggagga gcaatcgtac ttaggtatat gagtttattt
     5401 aatggatatg gagtgtcaaa attggtatta gctgcagctg ctgcaccatc atttgtacaa
     5461 agacctccag aatttccata tggtatgaca cgtgaagatg taaataaatt gatagcacaa
     5521 gcatgtactg atagaccaga aatggtaacc gattttggcg aaaaagtatt tgcatcaaat
     5581 ccaccagaaa gctttagaag atggtttaat gatattggtt ttagtgcatc aggtattggc
     5641 actattggaa ctgctgtatc tttaagagat gaagaattat ttaatgattt aaaatgtgta
     5701 agagtgccta ctggtatatt tcatgggaaa ttagatgaaa tatgtccgta tgaatttgca
     5761 gtttttatga atgaaaaaat agaagattca attttatata cttttgagta tagtggtcat
     5821 gctatattct atgatgagct taaactattt aatcaggaat ttttacagtt tttagaggaa
     5881 tagatatatt ttattatcac ttagaaaaag agtaatatta tgtgaaaaga tttaagctaa
     5941 attttatgat gttttttaca ctaaacaaat agggttttag aacttgtaac taagttctga
     6001 gaccctatta ttatatattc ctaaaatttg ttataa
//
LOCUS       DAYAOX010000218         6032 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.219, whole genome shotgun sequence.
ACCESSION   DAYAOX010000218 DAYAOX010000000
VERSION     DAYAOX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.219"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            104..550
                     /locus_tag="ACGI57_003168"
     CDS             104..550
                     /locus_tag="ACGI57_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718224.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEELDLHEDAKKDNIYKEDEYKKYEEE"
     gene            814..1539
                     /locus_tag="ACGI57_003169"
     CDS             814..1539
                     /locus_tag="ACGI57_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9718225.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            1517..2770
                     /locus_tag="ACGI57_003170"
     CDS             1517..2770
                     /locus_tag="ACGI57_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9718226.1"
                     /translation="MKIVFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMV
                     VRNNQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPI
                     ISKLVLETIKINIILVCIIFILIFVLIVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKN
                     KNQEGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLND
                     LLYNDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHR
                     AMLSMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITY
                     KENPLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNG
                     DIYVNSEKGKGTEFHIIFHKMYDSD"
     gene            2850..3527
                     /locus_tag="ACGI57_003171"
     CDS             2850..3527
                     /locus_tag="ACGI57_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718227.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQVSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            3528..>6032
                     /locus_tag="ACGI57_003172"
     CDS             3528..>6032
                     /locus_tag="ACGI57_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718228.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALIYGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVKNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIVCTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIP"
BASE COUNT         2529 a          551 c         1018 g         1934 t
ORIGIN      
        1 ttatattttg taaaaattgt aatttaaaaa ataacataat ttttattaaa ataatacaga
       61 tactatttat agatagaaat aaaaattaga aaggagaaaa aatatgttaa catatttaat
      121 gaaaagacaa atgcataaaa aagatgatgg aatgagtaaa ccaatgatga ttacaggagc
      181 tgtaattaca ggtgtaggag catatgctgt atataaaatg gtaaaaagcc ataaatctaa
      241 ttctcaaatg gtcaatacaa attattatgg aaatagcgat tatgatgatt ataaatatga
      301 cgaatttgat tatgactgtg attgtagaga cacaaaacaa gattatgaat atgaagaatt
      361 aaaaaaaaaa gtaaaagaat ttaattcaag aagaataaat tgtgaaaatt gtccacatgt
      421 ttctcaagaa gaaatgatgc aatatgtaaa tagtgttaaa gatgtaaagg aagagcttga
      481 tttacatgaa gatgctaaaa aagataatat ttacaaagaa gatgaataca aaaaatatga
      541 agaagaataa gtaatatttt gtataactga acaaatagta atgtcaaatt atttaatatc
      601 tagttttaat atgtaagttg taacttaact tgaaagaatc agtatcattc tttagatagt
      661 taagtaacaa cttacatttt atttttataa attttaatag ttctttgtga ctattttgta
      721 attttagaaa tattttagta tccagggtaa ttgtgtataa taaaacttgt atagatagat
      781 agtatgaaat taagcagata gtttaaaatt aaaatgaaaa gaaaggaaat agttatgtta
      841 aagttgttga tagttgaaga tgatagtact atatcttttg gaataaagta tgctttagaa
      901 caagaaggat ttagtataga tatatcaaag gatttatcta gtggaaaaga aatgatttct
      961 tctaatgaat atagcatgat actgttggat gttacattgc cagatggaac tggttatgag
     1021 ctttgtcaat atataagagg attctctcag gttcctataa tattcttgac tgcttgtgat
     1081 gaagaagtaa atattgtaat gggtctcgat ataggtggag atgattatat aacaaaacct
     1141 tttagaatta gagaattaat atccagaata aaagctgttt taagaagaaa aggtaatact
     1201 tcagaagaaa ataagaaaat acttaaattt ggagatttaa gtatatatac attagaagct
     1261 agagtatata aaaatgataa agaaatattt ctgacatcag ttgaatataa acttctttta
     1321 attcttatac agaataaaaa tactgtttta agtagaactc aaatattgga aaaactttgg
     1381 gatgtaactt atgattttgt aaacgacaat actctaactg tttatataaa aaggcttaga
     1441 gaaaaaatag gtgatgattt atgtgaacca atctacatag aaactgtaag ggggattggt
     1501 tataaatgga taggaagtga aaatagtgtt tctatataat ccagaagtga agaagttttt
     1561 aagtaagtat gttactttga tgtttattgt tatcataata tctattggat ttagcataat
     1621 caacgtgtcc ttaactaaag agatggttgt aagaaataat caggctataa taggaacttt
     1681 atcaagcaag tatccaaact tagaaagtca gatagtagac ataataactc aagggaagtc
     1741 tatggagaac atagattatg gtcagaaaat attaattaag tataattatg ataaatctat
     1801 taggataaac tctgagccta ttatttctaa attagtttta gagactatca aaataaatat
     1861 aatattagta tgtataatat ttattttgat atttgtgttg attgttagat attttacgtc
     1921 aatttacaat gatttatcag atatgactaa gtatgtttac tatagttcag agggtaagcc
     1981 atttgatatg aaaaacaaga atcaagaagg gcagataggt cttctaaaaa ctgaactctt
     2041 aaaaatgacg acaattctta atgaaaaggt tgaactttta aaaagagaaa agatattttt
     2101 aaacaataca atttccgata tatcacatca attaaaaact cctatgactt ctttaataat
     2161 gttgaatgat ttattatata atgatgtacc atacgaagta aaaatcgatt ttttaaataa
     2221 gataaaaaat caattaaata gaatggattg gttgattaaa agcatgctta aattatcaaa
     2281 ggtagaagca aaagtgataa attttaaaaa agataaggta aaatttagtg aactcataca
     2341 tagagcaatg ctatctatga aaatacctat ggagattaaa aatcaaaaac tgactataga
     2401 gggaaatgat aatgtatctt atattggtga tattgattgg tccgtggaag cattagtgaa
     2461 tataatcaaa aattgtgtag aacatactcc tgaatttgga aatataatta taacctataa
     2521 agaaaatcca ttattttctg aactaataat aaaagatgag ggtgaaggta tagataaaaa
     2581 agatatacca catgtattta aacgattcta tagaggaaga agtagttcaa aagaagatag
     2641 tgtaggaata ggtcttgcaa tgtcaaaatc tataatagaa agccaaaatg gcgatatata
     2701 tgtaaacagc gaaaaaggta agggtacaga atttcatata atatttcata aaatgtatga
     2761 tagtgactaa tctgtaatat ttaattcact ttatagtaat agccaaaacc taatatatga
     2821 agtatagaat ataaatattg gaggtagtta tggaaatttt aagagtagaa aatctaacta
     2881 aaagttatgg taaaaatgaa acaaaagttg atgctataaa aaatgtaagt ttatcagttg
     2941 aaaaaggaac gtttatagca ataacaggtc caagtggaag tggtaaaagt accttattac
     3001 atttattagg aggagttgac agaccaacta gtggtaaggt ttatataaat gatgttgata
     3061 tatataattt aaaaactaag gatttggcga tatttagaag aagaaatata ggtcttatat
     3121 atcaatttta caacttaatt ccagtcttaa atgtgaagga aaacatattg cttccagcag
     3181 agcttgacaa tagagatatt gatagagaat attttgatga tttaatgaaa acattaggtc
     3241 taattgatag agaaaagcac cttccaaacc aagtatcagg aggtcaacaa cagagaacct
     3301 ctataggacg tgcattaatt aatagacctt ctataatatt agcagatgag ccaacaggta
     3361 atttagatag taaaaattca aaggaaatat tagaattgtt aaaattgtca gtaaaaaaat
     3421 acaaccagac actaataatg ataactcatg acaaaaatat ggcacttcaa gctgatagaa
     3481 ttataagcat agaagatgga atcataaaaa gtgatgaggt gatgtagatg aatttatata
     3541 catcactaac tattagatac ttaaaacaaa ataaaagaag aaccattgta accataatcg
     3601 gtataatact ttcaacagct ttaatttatg gtataggaaa tatatttgaa agttttatgg
     3661 attatcaaat gagagaaact ataaagaatg atggaagttt ccatgttaca ttttatgatg
     3721 taaatagaaa aaatgtggaa tatgtaacga aatcagctga aatagaaaaa catgcattta
     3781 ccaaacaact tggatattct aaattagaaa atagtgaaaa tgctatatta agtataaagc
     3841 aatatgataa aaatgcaata aatggatata aagtttctat aaaagaaggt agatttccag
     3901 ctaaggaagg agaaattgtt ttaagtgaaa gtattattaa tcttatagat gataagttaa
     3961 aaataggaga taagataact ttaaaagtag gggatatgtt tgactcaagc aagaaaaaga
     4021 ttgatagtat gacatttcat gaaggtgatt atatagcaaa tgaaaaagat agaactttta
     4081 aagttgttgg tataattgaa aaacctgggt ttgaacgtta taatggaatt gcaacagcta
     4141 tagcctactt taatcctatg aataattaca atgatacaat taatgtatca gtagcagtaa
     4201 aaaatcctaa agatgtgtat aagataagta ataaaataag taaaaatata tattcaaata
     4261 aagataaaga agcagtttct gatatgataa aatacaatga acatttattg aggttacaag
     4321 gtgctagtaa gtattcaaat ataaatagct ctataaagtc tataattata gcagtaacta
     4381 ttttagtaat cgtatgtaca attgctacag tgtacaattc ctttagtatt tctataaatg
     4441 aacgaaaaaa acagtttggg atattaaatt ctataggtgc tacatctagt caaattaaaa
     4501 gattagtttt tatagaaggt attataatta gtttaattgg tataccaata ggactaatat
     4561 ctggaacagt agctatagac ttattgttta aaattataaa taagtatttt actgaatcgg
     4621 ttgttactaa gatgagtttg cagatagtgt ataatccaat aataattatt gttagtataa
     4681 taatagtttt gcttacaatc tttatatcaa ttctactacc tgcaatatcg gcttctaata
     4741 tttctcccct taatgtgatt aaaaatactg gagagtataa agtgggcaaa atcaaaagct
     4801 caagacttat taaaatgatt tttaaaactg aaggagtact tgcatataaa aatattagga
     4861 gaaatagaaa aaaatttata ataactcttt tttctttaat ggtaagtata ataatattta
     4921 tatcttttag tggctttaca ttacttttac taagaggtga agaaattaga aattctcaaa
     4981 gaaaatatga tttgtattta actgcaaaga acacagctag gtctgtagag gatactataa
     5041 gtgaactaga agatattgat gggataaaaa actttgaatt ggcaacagga caatatgtgt
     5101 ctataagagt aagtgaaaat aaaataaata aatccaagga agatttaatc aggaaatatt
     5161 atcaaaaata taagattgga gatagttatg aatatgattt cagtaataat gaattaattt
     5221 ttccaggaga ttttgcagta aaaaacaaaa taaataactt agtacagggc tcttttaata
     5281 aagagagagc aattgaagaa aatggtgtta tattagttag aaaaagtgca attgaagaaa
     5341 atgggaagaa aggtgtagta gaacttacca actataaagt aggagataca gtaaattgtg
     5401 aatatcttga tgaaaatgcc ttaagtaaaa aagtaaaggt caagattctt gctataactg
     5461 acgaagaacg tctaggtctt ggttatcaaa atatggggtt acaatttata acttatgatg
     5521 aagttgctaa aaatttaaat ttaaaattaa gtaaaagcct tatatttatt gattcaggag
     5581 gaagcattaa gactaagaag gaaatagaag ctttggcaaa taaaaataat tttaactttt
     5641 atgatgaaag tgcaagcaat gaaagtgaaa aacaagattt aaaggttatt aaaatatttg
     5701 tatatggttt tatagtagtg atatctttag tgagtgttac aaatattcta aatacagtaa
     5761 gtacaagtat aaatctaaga aaaagagaac ttgcagtaat acaatctata ggggttactc
     5821 ctaaaggatt taggaaaatg atatatttag aaagttttat atatggtata ctgtcattgt
     5881 tgtttggaat accaattagt attggtataa ttctaattat gaataaactt atttcaggtg
     5941 taattgaatt ttctccgatt ataccgtgga cagccatagt tatctgtata gtaagtgttt
     6001 ttataataac ttttatagca gggtatatac ca
//
LOCUS       DAYAOX010000219         6028 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.220, whole genome shotgun sequence.
ACCESSION   DAYAOX010000219 DAYAOX010000000
VERSION     DAYAOX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.220"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            118..243
                     /locus_tag="ACGI57_003173"
                     /pseudo
     CDS             118..243
                     /locus_tag="ACGI57_003173"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
     gene            298..564
                     /locus_tag="ACGI57_003174"
     CDS             298..564
                     /locus_tag="ACGI57_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFG9718229.1"
                     /translation="MAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDYLIKVL
                     AEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            613..1197
                     /locus_tag="ACGI57_003175"
     CDS             613..1197
                     /locus_tag="ACGI57_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HFG9718230.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1202..1525)
                     /locus_tag="ACGI57_003176"
     CDS             complement(1202..1525)
                     /locus_tag="ACGI57_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9718231.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1677..2195
                     /locus_tag="ACGI57_003177"
     CDS             1677..2195
                     /locus_tag="ACGI57_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9718232.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2371..3039)
                     /locus_tag="ACGI57_003178"
     CDS             complement(2371..3039)
                     /locus_tag="ACGI57_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFG9718233.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3154..4569
                     /locus_tag="ACGI57_003179"
     CDS             3154..4569
                     /locus_tag="ACGI57_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9718234.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4768..5250
                     /locus_tag="ACGI57_003180"
     CDS             4768..5250
                     /locus_tag="ACGI57_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9718235.1"
                     /translation="MKTNTDVIMEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFT
                     NVVGCTIHQYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQ
                     VYRNKDEFYPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA
                     "
     gene            5247..5831
                     /gene="clpP"
                     /locus_tag="ACGI57_003181"
     CDS             5247..5831
                     /gene="clpP"
                     /locus_tag="ACGI57_003181"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFG9718236.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
BASE COUNT         2310 a          673 c          962 g         2083 t
ORIGIN      
        1 aggaaaaata acatatttgt attaaatttt attgaaatga ttaaatttat ttacttaatg
       61 taaattatta gctaaaattg tattaaataa ttaaggaggt gagtagctct ggatgaaata
      121 gatttaaaaa taattaattc tctaaaaaag aatagtaggg cttcaacctc tgaaataagc
      181 agacaaatta atttatcagt accagcagtc tcagagcgta taaggaaaat tgagcagtca
      241 aatttaattc aaaagtacac tattcaaatt aatcgggaaa aaacaaaata taagcttttg
      301 gcttttatat ttatcaatac tgatttatct aaaagtatga aagaatttaa gaaaactata
      361 ttggaatatg atactgtatt agaatgtcat catatttcag gagagtatga ttacttaata
      421 aaagtattag cagaagatac aagtaatctt gaagagttta tatcagtgac tttgaaggaa
      481 attgggggga ttcaaaaagc taatacaacc attgttttat ctacaattaa agaagaattt
      541 aatacaataa aagatatttt ttaaataatt tactagtaaa gtgttaatta tattttagga
      601 ggaaaataga atatgaaagt tttagttttt ttagcaaaag gttttgaaac tatggaattt
      661 agtgtatttg tagatgttat gggatgggct cgtaacgatt atgggcatga tattgatgta
      721 gtgacttgtg gatttaaaaa acaagttatg agcacattta atatacaagt attagttgat
      781 aagacgatag aagaagtatg tgttgatgat tatgatgctc tagctattcc tgggggattt
      841 gaagaatttg gattttatga tgaagcatat gattcatctt ttttaaatct aataagggag
      901 tttaattcaa aagaaaaaat aatcgcttct atttgtgtgg cagcattgcc agttggaaaa
      961 agtggtgttt taaaaaatcg taaagctact acatatcatt tgaaaaatgg taaaagacaa
     1021 agacagttaa gtgagtttga tgtaaatgta gttaatgaac caattgttgt agataaaaat
     1081 attataacct catattgtcc agaaacagct ccacatgtag cttttaagct tttagaaatg
     1141 ttgacatcta aagaacaaat ggatgaagtt aaattagcaa tgggatttaa actgtaatgg
     1201 tttatagatt ttctaagtaa tcagaacccc agtcacacat ggaatctaaa attggaataa
     1261 tactctttcc aaaatcagat agcgagtatt ctacttttgg aggaacaaca ggataaacca
     1321 ctcttataat taaaccatct gattctaatt cacgtaattg ttgagttaac atcttaggcg
     1381 ttgctttagg aattaatttt tttaattcat tgaacctcaa tatagtgtct ttaagatgcc
     1441 aaagtataag tgtcttatat ttaccaccaa tcaatgcaag tgttgcttct attgggcaac
     1501 tgtaattatc taaacatttt ttcatttttg ggctccttta tttattttaa agtatctttt
     1561 taggtactat attacaaaaa agtgcattat tgtaatatag taccatagtg ataaaattat
     1621 agcataggtt aaataaatca tcaacttagc ctaataaaac aataggaggt aaataaatga
     1681 atttcgttga acttgcaaaa aaaagatatt cttgccgtaa ttatcaagat agaaaggttg
     1741 aaaaagaaaa acttgagaag gttttagatg ttgcaagaat agctccaact ggtggtaatc
     1801 gtcaacctca aagattgatt gttattcagg aaaaagaagg tataaataaa ctttctaagg
     1861 ctgccaatat atatgatgct ccattagcaa ttcttgtatg tggagataaa gacaaagttt
     1921 ggacaagacc ttttgatggg aaacaactta cagatattga cacaagtata gttacagacc
     1981 atatgatgtt acaggcaact gaacttggat tggcaagtgt ttgggtgtgc tattttaatc
     2041 cagatataat tagagaagag tttagtttgc cagataatct agaaccaata aatatattat
     2101 tgatgggata tgaatctaag ataccagaga gcccagagag acatgaaaaa actcgtgttc
     2161 ctttaagtga aattgtttct tatgaaactc tttaattata aaattaagtt attcatagga
     2221 taaatgtaat aaagtgttta ttataaacaa aattcaaaat aaataatatc tacatataat
     2281 taattttagt gaagtatatc tagtttaact agaatgtata cttcactaat ttatttattc
     2341 aaaatattta tgaggatttt aatatattaa ctaaaatata tcaattgaat taacttttaa
     2401 atctggaaaa gcacttgaaa tatagtgttt tatgtcattt aaatggtctt gaatttgctt
     2461 ttctgatatt ttatcaggtc catatacaaa atacataata tttttagtat cttttgttat
     2521 agctgttcga ttatcagggc cattaagaat actagataat ataccaatat tatctgataa
     2581 aaataaatca tcttttgtta aaatatgttc tttaccacct ataccataaa aatgttcact
     2641 tccatttgct aattctattt tcaaaggtaa ttccatttta tcaaggtcat gacctgctgt
     2701 tagaagaaga tttttcaatt ctgctaaaaa cattgactca attgttacac ctacatctgg
     2761 gatacctttt gattttaata gaatagattc taattgaagc aatacagggt aagttttctt
     2821 gaatttttta taatacttta tataacttga aacaggctct gttttaattt tttctttacg
     2881 attgtattct gggtgttgtt ctataatatt tttaattgta gagtttttaa gaactaaaaa
     2941 attttcttta ttaggttttg aatgtgtaat atttataacc ataactccaa atttaatatt
     3001 gggataaatt tctttaactt tatgtgatat ttctaacata atatagactc ctttttattt
     3061 tatattttat gatataatct tagcaaatta aaagtggatt ttgtagttcc atttttatag
     3121 taattttata gaaccacttt tggaggtatt agaatgtgga ttacgattaa tagaaatagt
     3181 aatatatcat tatcaagaca aatttattgt gagataaaag atatgattct ggatggaagc
     3241 ttaaagtttg agcaaaaact gccatcatct aggactttat caaaagaact atctgtatct
     3301 cgtaatacag ttttagatgc atataatcaa ttaattgcag aaggatattt ggaaacaagg
     3361 aatggatatg gaacaattgt agctaaagga atatcagggc ataaagttga ttttaagtta
     3421 aatgatactt ttagagatga aaaagattta catactaata aacaaaaaaa ttatatagat
     3481 tttcgttcag gagttccaga attaaaacta tttccacgca aagaactttc aaagctatat
     3541 aaacaaatac tgtatgattt atcagattcg gatttcagat atggaagtac agctggtgta
     3601 tgggttttaa gagtggaaat atctaagtat ttatttcgta ctagaggaat tagatgtaat
     3661 cccaaaaaca ttatgattgt ttctggttca acacaaggtt tatctttaat ttcacagctt
     3721 ttatacaaaa gtaataaaaa ggttgctgta gaagacccta ttcataaagg cttattaaag
     3781 gttatttcat ctattggata ttcagttgtt ggaattaaag tagatgataa gggaatgaat
     3841 accaacttaa taaaatcaat aaaggggatt tcttttgttt atactacacc ttctcatcaa
     3901 tacccattag gagggatttt acctattcaa agaagactag agcttattaa atgtgcaaca
     3961 gagaatgatt actatataat tgaagatgat tatgatagtg aatttagata tgagggtcaa
     4021 ccaattagtt ctctttatga acttagtcca aataaagtaa tttacatagg ttcttttagt
     4081 aaaatattat ctccagctat tcgtgttgga tttatgttat taccagacaa tattttagaa
     4141 agctataaaa ccttaaaaat gtactctgat gttcatacag aaacaatatt gcaacatgta
     4201 ttagctgaat ttattggaaa tggaggttta gagaagtaca tatttagaat gaagaagttg
     4261 tacaatagaa aacgtaagta cctcataaaa gttttaaaca cggaatttaa aaatgaattt
     4321 aaaataaaag gtgaagcagc agggcttcat atagttgctc atttctataa tgttaaattt
     4381 acggaaagtt taattcataa aatttttgaa gcaggtataa aagtttatcc tatacaacaa
     4441 tatgcaattg agaatatatt agaatatgac tatgaaatta tactaggtta tgctcatctt
     4501 agttttgatg aaattgaaaa aggtgtaaaa atactcagca aaataataca tatgaatcaa
     4561 ggtacataga taaaacatgt agcaatctac atataataga tgtaattaag tttaaacttt
     4621 aaaagaaata tatattaata tttttgtata aaaatacctg atattttttt atcgatactt
     4681 tattgctata cactaaatta agatagcaat ttatattaag aaaaaacttt ttgcataggt
     4741 tataatactt aaaaggagag gatagagttg aaaacaaata cagatgttat tatggagata
     4801 attgattata tagaagaaaa tcttacagaa aatctaaatt tagataaaat agcttatatt
     4861 gctaattatt caaaatacca tttacacaga atgtttacaa atgttgtagg atgtactatc
     4921 catcaataca tacagaggag gagacttact gaagcagcta gacaacttat atttacagac
     4981 aaatatatta tagacatagc tattacagta ggatattcta cacatcaatc ttttacacta
     5041 gcatttagaa atttatataa agaatctcca caagtatata gaaataaaga tgaattttat
     5101 cctattcaac agagatttgt tttaaatcta aatcatcaaa ttcaaagtga aaaatgtatt
     5161 tcttatgcaa atatgaatta taaacaaaga agagatgaaa tcaattctta tctgaacttt
     5221 aatattaatt gtgaggtgaa agtagcatga gtttagtgcc acatgtaatt gaacaaacag
     5281 gtcaagggga aaggtcttat gatatatatt caaaattatt aaaagataga attatattta
     5341 taggagaaga aattaatgat gctattgcaa gtttagtagt agctcaatta ctgtttttag
     5401 aatcagaaga cccagattca gatataatta tatatataaa tagccctggt ggctctgcta
     5461 catcaggatt tgctatatat gatactatga attatgtcag atgtgatgta tcgactattt
     5521 gtattggtat ggctgcaagc atgagtgctt ttttacttgc aggaggaaca catggaaaaa
     5581 gatgtgcttt acccaattca gagattatga ttcatcagcc aatgggagga gctaaaggtc
     5641 aagcaacaga tgtaaaaata gctgtagata atatattgaa aataaaagaa cgattagata
     5701 aaattctttc tgaaaataca ggtaaatcaa tagaagatat taggagagat acagatagag
     5761 ataattttat gactgcacta gaggctaaag agtacgggct gattgattat ataatgaata
     5821 ggaatgaata aagtatcaaa gtatcatatt aaaatgtctg ttttacaaat atatatataa
     5881 tattaaataa gagtgtctca aaatgaactt tttagtttat gggacactct tttttgtatg
     5941 aaattaaata tggttttatc atttcgacaa ttattaccaa atttcaagtt taataaattc
     6001 tactataaaa aatacataaa agataagg
//
LOCUS       DAYAOX010000220         5974 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.221, whole genome shotgun sequence.
ACCESSION   DAYAOX010000220 DAYAOX010000000
VERSION     DAYAOX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.221"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(22..1092)
                     /locus_tag="ACGI57_003182"
     CDS             complement(22..1092)
                     /locus_tag="ACGI57_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFG9718237.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(1085..2143)
                     /locus_tag="ACGI57_003183"
     CDS             complement(1085..2143)
                     /locus_tag="ACGI57_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFG9718238.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(2130..2462)
                     /locus_tag="ACGI57_003184"
     CDS             complement(2130..2462)
                     /locus_tag="ACGI57_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFG9718239.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            2661..3782
                     /locus_tag="ACGI57_003185"
     CDS             2661..3782
                     /locus_tag="ACGI57_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9718240.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(3910..5808)
                     /locus_tag="ACGI57_003186"
     CDS             complement(3910..5808)
                     /locus_tag="ACGI57_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HFG9718241.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT         1900 a         1006 c          651 g         2417 t
ORIGIN      
        1 taaattatta tagtaaatta tttacaattc taagctattt gaatttattt ttatagtata
       61 ttctttctct aaattcgaca gatttttatt caaaagccct tttatttccc tcgtatttct
      121 atacttgttg ctatgttctt cattatgttc ataatcatat gaatcattgt catattcttc
      181 atcattatta aattcttcaa ttgcttcttt tgactttata tcacaattaa atttatactt
      241 atttactggt atatcaagtc ttaccttaga atttggaact tctatgtcca ggaacttagc
      301 aattggcata tcagagtaca agtaaaattc actttcataa tttgaatttc tacctatttt
      361 ctcttttata tttacatttt caggtatata ctcttctata ttattgataa atacatctcc
      421 attctctgaa gatatatcca atgactctga ttctatgttt acatttttaa cgcctaaaat
      481 tgactttgtt gatagatgaa gattgctatt agatattaag tttaagtttt ctaatctatt
      541 tactttattt tctgttatag ctctgctaaa acctccagaa gaagttttat atgttatatt
      601 atttgaatct acatcatcag atataaataa gtacgaactt atacttgatg catttatatc
      661 tacattattt ggtacatata ttctaatttc tctataatca cgtgaaaaca tactatttat
      721 agatgtattt aataagtggt cgaaacttct aatcttttta attttatttt cagattttct
      781 ttcgcctttt acaactaaag tcttatcttg tagggttaca ctgtatttgt aaaattcatt
      841 attccctctt gttgtcacac gcacatcttc atttgaactt tttcttatca taacatttat
      901 attgtcaaca gctatatcta acttgtcaat atttactttt tcttgataaa gtttgttttc
      961 tttgtttaat tcagcttcct tttctaaacc aaagtttatt aggcttggca taactaaaat
     1021 ccctgaccat gttgtcccta ttatacctat taccaataaa actgcagcaa atatagcaag
     1081 ttttttattc atgtttatct tctcctttct tattttctat atcttcaagg tccttatata
     1141 tcgcctcaaa atttatacta tcatcattat attcatcagt ttcttttatg ctttcttctt
     1201 ctctattgta gtaatcatta ttatatgaat tttcattaat gtctttattc aaatcttcat
     1261 catttaaagt agcttttttt cttttttcca tcttttcatt tgcctttata taaatatttt
     1321 ttctctttat ccaatgtata taattattta gtaattgttt acctagtttt atggcaaata
     1381 ataaaatttg ccatgcaagt atctcaagcc ctacaaatgc tatggttgcg aaaatacaaa
     1441 acatcataac atctggaacc attgctataa atttaatcac aaatggaatt aatataacaa
     1501 aagcaattaa tgagcatact aatgcaatag tagcagatac aatagcacat acaaacgcaa
     1561 atgctggaac aattagaaat aaagataaca ctactaataa cccctttata acttgtctgg
     1621 ataatcctcc tacaccttct tctatacttg gtgtaagttt atcatttata ttttcttttg
     1681 atttattgta cttttcctta gcttcatctt tcaagataga ataatccttt tttacatttg
     1741 atttaaatct actaaattta ccatttaata tgtctgtatt cattattttt ccatctatat
     1801 tttcttttga tttactatac ttttcttttg cttcatcttt taagatagag taaccttttt
     1861 tcatatctga tttaaattta ctaaacttat cattcaaaac atctgtattt attatttttt
     1921 cctcttcaat atttttttct ttagtttgta agataagttc ttttgctata gattttggtg
     1981 agccaagtga tgctattatc tccatatcac tctttccctc tataagacca tctacgaaat
     2041 actcttcata atctctcaat atatcattaa cttcgctctc agaaaaatct ttttttagat
     2101 aatcctttaa aatttcaaga aactcagttt tattcaagtt cattacctcc ctcatcttca
     2161 cttattaaaa tctcaacagc ctttacaaat tctctccact cttccatcag attggataaa
     2221 ttctcttttc ctagtgaagt tattctatag tattttctag caggaccttc attagattct
     2281 agtatatatg tttcgaaata caaatctttt gtaagtcttc ttagaatagg atatattgtt
     2341 ccttcattta catctattac tttagagatg ttatgtacta tctcatatcc atacatatct
     2401 ttcttagata tgagagcaag tacacaaatt tctaaaacac cctttctaaa ttgtgtgttc
     2461 atatactcca tcctttcttt aaatacacag tattgtgttt tacaaggtac tatgttaaaa
     2521 cttcttatat cctcataata acacagtact atgttctgca caatagttat ttttaaatat
     2581 tcttgttgat taaatccttc tataaatgta taatactcta atcagatata ttttatgaaa
     2641 gtggtgataa attggctaaa atgaaagatg ataaaacaat attaatccta actgctcaat
     2701 ttggtgctgg acatataagt gctgcaaaag cagtcaaaga atgtattatt gaaaaatata
     2761 gtaactataa tgttgttata caaaacttta taaatgcaag tattcccatg atgaataagc
     2821 ctatggtaaa actttatgaa aacaatacaa aatatactcc tggattatat aattactatt
     2881 attactttaa gaaatcgttt gattcaagac atgatttatc tcataaatta tatacaccta
     2941 aactttctga gtatattgct gatataaatc cagatttgat tatctctaca tttccactag
     3001 ctgctgcttg tgtaaataac tttaagataa aaaatcctga tataaatata cctacactta
     3061 cagttataac agatgttgta gatagtatgg aatgggtttt tgaaaataca gatttgtatt
     3121 ttgttccttc tcctgaaata aaaaatagat tttttcagaa aggaataaat ccagactcca
     3181 taaaggttac tggagttcct gttgataaga gatttcaaat tgaaagtaaa gaactatgtt
     3241 gtgataaata tagactatta cttctaggtg gaggaagagg tctatttgat atagatgagg
     3301 attttatgca ttggatagat gaatttattg aagaacatag taattctata gaaattacaa
     3361 tagttactgg taaaaacaaa aaattatatg acaatctaac tcataaaaaa cctctaaaaa
     3421 atattaaagt acttggattt gtaaacgaca tgtataatct aataagagaa tgtgacctaa
     3481 tgctaactaa gcctggagga gcaactatat ttgaagctat tcaatcacaa actccagtac
     3541 tcgtaaaaat gccaaaagtt ggacaagaaa ttgaaaacgc taaatttata atagataagg
     3601 gtcttggcat gatttatagt gatgacctag accttaaaaa catattttat aaattagtat
     3661 caaatgaatt tgattctata ataaacttta tgaaaaaaaa tttaaaagaa tttaaaacag
     3721 tcattcatcc agagaaaatt gctgattata tatcagaatt aatagataat cattacagtt
     3781 aaattgtact attaaaaaat ttcaaacata aaattaaagg caatcaagta tgcttttaca
     3841 cttgagaatt tactcatagt gtttaataat acttgattgc ctttatcata tcaaaatcaa
     3901 attgttatat tagtatatat ttttcttgta ctgcatatac agttgttgta tttcttccat
     3961 acttctcttt aattctattt gtaaatctga agcagtctgg taatccttat tttctatagc
     4021 ttctctcact ctagtgaata gtgattctct agttaaagta tccttcataa tattgaactt
     4081 taaatccgat attgagttcc aaactacctc atcaagcgca tccatatttt cttttgtgtg
     4141 tatcttgaca atacttctta ttttttgaat cccagcctct attattctag tttttaattt
     4201 cttttgccac ttatctatag ctccaatctt aaatgattct attatactat cagtaaatac
     4261 atcaccttgt tttaaagatt ttaattttga agcatatatt tctaagtttt tcatattttc
     4321 ataaactgta gccggtggta ttccaaatct tgcatttctt tcttccaaac tataatgttc
     4381 aaatacatca ttttcatctc tatatgctct atctttttct aaatagaaac tttcttcacc
     4441 tacattttta gataactctt tttcaagttc ttttgtacta agaccactct tagctgctgc
     4501 ttttatacca tccaacattg tttgataaca tcctgctatt actaaatacg tgttagatag
     4561 agggcttggt gaccttaact caaatcttac agtctttgga tttttcatat ctcttattaa
     4621 tccaacaagc actgacctat ttcttgatgg aatctcataa ctatgaccta aagatgtaac
     4681 tatgcataca ggagcttcaa atccaggaac taatctatta aatccatcat tagaagctgt
     4741 tacaattggg tttaatactt cataattgta aagaagtccc ataagagccc catatcctag
     4801 ttcacttaga taatcttctt ttaaatcttt tggcgcaaat agatttttta tacttccatc
     4861 ctttaacttc gcacttactc ctacatgtgt atgtccacca cttcctgcta ctccatgaat
     4921 tggttttgct ttaaatgtaa cttctagtcc atgtgatgta aatacatcct ctataacatc
     4981 tcttactaat aattcattgt ctgctgcttg taacggtgta gaaaacttcc aagaaacctc
     5041 taattgctcc atagcatggt ttgttcttcc atctatacta atagaacttt gtattcctcc
     5101 gacctctttg tgagccattt cagggtttac acctagcttt tgaagtataa ttaaactttt
     5161 ctctaaacaa gttcttataa taccatgagt tcttttccag tattgctctt ttaggctttg
     5221 tgatacatat aatttctcta agtcagcttt atcctcagga gtattaaccc agaactcaag
     5281 ttctgtagct gctgttaaca ttatttcctc tatatcatct actgaatcta ttcctatgtt
     5341 atttattaca tgtggatatt ctctaaatat ttcatacatt gactttttaa agtacttatc
     5401 tgctttttga agtactcctc tagaacatac ttttttatta tcatgtatta aaaatgcagg
     5461 tatctttaat gttccaacag gtagacctac ttcttcgtct atatattcca tattgtaatc
     5521 tacataccaa tgacaacttt tatctggcat taaatctact ttcgcattat ttaatgtagc
     5581 tatattataa agctcaacac ttgaaccatc agtctgtata gctgattcca aaaatttatc
     5641 tatatcctcc aaaaataatt ctacaggaat cttttcatct gttgcatttc ctcctaaatc
     5701 aacccccatt aaggaaacaa atcttatatt tttattgctt tttaggattt ctcttaattc
     5761 ttcattagtg tgtttatctt tctctattac gtataataga ctatccattt ttccactctc
     5821 ccataatttt tatgtatatt ttattttgtt aaatacaaga taaatttcat tttccttaca
     5881 tttatgaatt tttcttgcaa attattcgtg ttttattcgt gtttttattt atattattat
     5941 atatcaagta taaaaagttc gcaatatttt ttat
//
LOCUS       DAYAOX010000221         5947 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.222, whole genome shotgun sequence.
ACCESSION   DAYAOX010000221 DAYAOX010000000
VERSION     DAYAOX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.222"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            554..1825
                     /gene="serS"
                     /locus_tag="ACGI57_003187"
     CDS             554..1825
                     /gene="serS"
                     /locus_tag="ACGI57_003187"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFG9718242.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            2103..3608
                     /locus_tag="ACGI57_003188"
     CDS             2103..3608
                     /locus_tag="ACGI57_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFG9718243.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3722..4420
                     /locus_tag="ACGI57_003189"
     CDS             3722..4420
                     /locus_tag="ACGI57_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9718244.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4705..5793
                     /locus_tag="ACGI57_003190"
     CDS             4705..5793
                     /locus_tag="ACGI57_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718245.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         2419 a          586 c          964 g         1978 t
ORIGIN      
        1 gcagtaagac tttggaacta aatctataat agcaaattta aatttattaa atagattacc
       61 ttataacatt tcatataaaa tatattgtga atctgagaaa attactattt ttctttgagt
      121 attttttgta agcagttcta aaagattcaa tttcatacct attacttgaa tcattcatta
      181 aaataattct aatttaaaaa ataattctat ccaaataata gatatatagg actatgttat
      241 aatataaaaa aatttatata aaagttaata actatacaag attgataaag aaaaagtcaa
      301 aatagataat tgattagcaa aatagatata attataatga agttaaatct agcaatattc
      361 tcctaaaatt aaattacatt tatttaatta aatataattt gacaatggac aaaaaagtga
      421 gtaaaatata aataaaataa aaataacagg ttcggataaa gaaaaaggtt tcttaacctc
      481 aagcataaag tatattatct aagttatttg agattaagag ataaatccaa tcttaggtac
      541 tgaggaggaa aaaatgcttg atataaaaag aataagagaa aatttagatt gtataaaaaa
      601 agcaatggaa agaaggggcg aaaaagactt taaccttgat gaagtagtta agttagatga
      661 tgaaagaaga aagattcttc aagaagttga agttatgaaa aatgaattaa atacagcatc
      721 taaaaatata ccaaatctta taaaagaagg taaagatgta gaaaatgaaa agattaaact
      781 aaaagagtta tctgataaaa ttaaggtaat agaccaaaat cttaaagaag tagaagataa
      841 gatggaatac ctattaatga gaataccaaa tgtaccacat ccagaagtac cacaaggtga
      901 aactgatgaa gataatgttg aagtaagaac ttggggaaag actactacct ttgattttga
      961 atctaaggct cattgggaaa ttggaacaga attaggtata cttgattttg aaacagctag
     1021 taaaataact ggttcaagat ttactcttta caaaggatta ggtgctagat tagagagagc
     1081 attattaaat ttttacttag acacgaatac aaaagtaaat ggatatacag aagttattcc
     1141 accatttatg gcaaatagaa atagtttttt aggtacagga cagttaccta agtttgaaga
     1201 agatatgttt aaaatagaag gtatggacta ttttatgatt ccaacatcag aagttccttt
     1261 aactaatata catgctaatg aaatacttaa atttgaacaa ttaccgataa attatacagc
     1321 ttatactcca tgttttaggt cagaagctgg ttctgctgga agagatacaa gaggtttagt
     1381 aagacaacat cagtttaaca aagtagaaat ggttaaaata gttgcaccag aagaatctta
     1441 caatgagtta gaaaagctta ctaacaatgc ggaaactatg ctacaattat tgaatctgcc
     1501 ttatagggta gtaaagatat gtacaggtga tttaggattt acggcttctt ttaaatatga
     1561 tgtagaagtt tggatgccaa gttacaatag gtatgttgag atttcatctt gttcaaactg
     1621 cgaagatttc caagctagaa gagcaggaat aagatttaaa agagataaag attctaaagc
     1681 tgaatatgta cacacattaa atggctctgg gttagctatt ggaagaagtg ttgcagctat
     1741 attagaaaat tatcaacaag aagatggttc tgtggttgtt cctgaagttt taagaccata
     1801 tatgggagtt tctgttatta agtaagagtt aaattatgta tttttaagca atacaataat
     1861 atagaggtaa taaaaaataa ttaatataaa agtgatacaa taatatagaa gtaataaaaa
     1921 aatagttaat ataaaagtga tacaataata tagaagtaat aaaaaaaaat aattaatata
     1981 aaaatgtgta caaactaatg aatattgata tagtaagcta tcatatgaaa gagtatgtac
     2041 acattttttt tactaatttt tcacaatatg aacataaata atttacagga aaggaatctt
     2101 ttatgaaaat tcaaaatact gagtggggat atattgaatg gaaacatact tatgatgaaa
     2161 ataatccaaa acaagctatg aatatataca ttgcagtaac tatgcctggt aagaaacatt
     2221 ttaatcatgt tcattatggt caagagcaga tgatatatat tttagaggga gagggtttgt
     2281 atattataaa tggagtttgg aagccatttt atcaaggtat gattttttat atagaatctg
     2341 gtagcacaca tgaaactata aatacaggtg atagagaaat aaaagaactt attgtttcaa
     2401 ataatgtaga tgatgtagga gagagtgaag ttatagatat taatcctaat aattatttaa
     2461 agaagacctt gataaattat agtgaaagta cattaaattt gtatgcagca gtagaatcaa
     2521 ttcgtggtca atttatagac ccgtttaaaa ttccattaat tatatatgat gattcatgga
     2581 atatagtttt aaaaaatcct tattttccac ttttttgttt tgaaaaatgt aatcctatga
     2641 aatttcctca aaattgtgat tgtatgaatc aaaaaagtag caatcaattt gtatgtgaat
     2701 atgggataac tatatataat ataccaatac tgtacaaaag caatagcata ggtgttataa
     2761 gaggagggta tgtactatta tctgatttga atttagatac agagcataat aatttatatg
     2821 atattccaga gggagctgct agaagtataa aacgcctctt gaaacaaata tctaaaaata
     2881 taataaattt ttgctctttt aatgatataa gaaaggattt acaagaaaaa gagaagacta
     2941 tagcaagaac atatcattat ggagagcaac ttgaaatgaa tttaaaagta gctcaagata
     3001 tggttactaa tttaagaata aatcatcact ttttatttaa tacattaaat tcaatggcaa
     3061 gcatagcact agatgatggt agttatgatt tgtatagtgc aataatagat ttatcaagaa
     3121 tgtttagata tacaatgagg tctgatttaa gatttgtaga attagaatct gaaatattat
     3181 acattaaaaa ttatttgaat ttacagaaat tacgttatgg agatgcactt aaagtaaaat
     3241 atcttattcc agagaagtta tataaccttt ctgtgccttt taactttatt caacctattg
     3301 tagagaatgc atttacacat ggatttagag atattgacac agaaaagcga attgaaataa
     3361 ttgcaagatt agattctcag tatgctatta tagagattca taataatgga actatcttag
     3421 atggaaataa tatagacaaa ataaaggcag gtattcgtag taataatggt catggtttgt
     3481 ctttaatata tacaaaattc acatctgctt atggaaataa ttttgatatg gatataaagt
     3541 cgagtgataa tgaaggaact tacataatga ttaaaattcc tattgaaaat tataaggagt
     3601 atgtatagaa cttgtgaaaa attaataagt tttataaatg ttaagaaata tattagatgt
     3661 ttttatgttg ttttatatat cttacttttt tatattatta agctaaaagg gaggtattct
     3721 catgagaaga gcactaataa tagatgatga agaagctgtt ctaaaaataa taacacattt
     3781 tatagaaatg aaagatatgc caataaaaat tgttggtaaa gcaacatctg gtgatgaagc
     3841 agtagataaa atcattggtt tagagccaga catagttttt atagatattc aaatgccaat
     3901 atacaacggg ttagaagtaa tagaaaaaac atctcattta tctaaaaaaa taaactttgt
     3961 agtaataact gcatttaact attttgaata tgctcaaaaa gctttgagat taaatgtaaa
     4021 agatatactc cttaaaccat tggatatgaa agaatttact aagtctgtag aaaaaattat
     4081 tggatatcaa tataccaata atgatttatt aaatgagata ctggaatata taaatctaaa
     4141 ctatagcaat gacttaaagt taaatgattg tgctagatta tttttaacca atccatctaa
     4201 tatatctagg atttttaaaa gtaatttaaa tacaactttt atatcatatc taaatcatat
     4261 aagaatagaa aaatctattg agttacttga aaatactaca aaaagtataa atgagattgc
     4321 agaaatagtt ggatataata gcctaaataa cttttataaa aactttaaga tagaaaaagg
     4381 aatgactcca aaagtttata aactgaacac taaaaattga aattgataaa taaaaagtca
     4441 atatagataa ttgtttgagt gtgtctgaat aagtatactt taattatgat aaaaattaaa
     4501 gcgctttaaa catatttaat aatattaaac cttataaaag ctgtacctat ttatataaaa
     4561 aaatatatat aaataaaaag aaaaagatat acaaaataaa aaaagtgaat taaatactgt
     4621 ttcaaaaaga cagaattttc atttaattta ctgagagttt atatttctta aaggaaggta
     4681 actttataat ggaggtaatt taatatgtca gacaaagtga atatgaaaaa tattatctgt
     4741 attgcaggcg cttatctagc aacagcaatt ggttctgggt ttgctactgg tcaagaaatt
     4801 ctacaatttt ttgcctcaca agggtatatg ggtttaattg gagcattaat atcaatggta
     4861 ttgttttcat ttcttggtgc tgaagttata actagaggta gagaattaaa acttaaagaa
     4921 ccaacaagaa tttacacttt ttattgtggg aagattttag gaaaatttta cgaatggttt
     4981 agtccaatat ttttatttgg gattcttata gtaatgattt caggtgcagg ctcaacttta
     5041 actgaatact atggtataaa tccttactta ggaagagtat tgatggctgt tgctacttta
     5101 attacagtgt ctatgggttt agaaaaatta gtaagcatac ttggacgtat tggaccagtg
     5161 atagcagtat ttacaattgc tgttggatta ataagcttgt ttaagcattt tgatggttta
     5221 tctatggttg gagaagctat gaaaaatatt gaagttacaa aagcatcaga aagtccaatc
     5281 gtttctggtg taatatataa tacattaaat attattgtaa tgattgtttt tttaacagga
     5341 atgggagctt ctattgaaag taaaaaggat gctttttggg gaggtttatt aggtggaata
     5401 gtgtttatga ctgctggtat agttatgtat ttggctatga tttcagatat aggaaatttg
     5461 tatatgaaag aaataccatc actttattta gcagataata tatctcctat tgtaggtgta
     5521 tgtttttcag ttgcattaat attaggtatc tacacaacag ctgttccact tttatggtct
     5581 gtaaccaata gaattgttga agatgaacat cctaaattta aattggtaac aacagtaata
     5641 gctattttgg cttgtatagg aggtttttta ccgtttggta aactagtaaa cattttatat
     5701 ccatatacag gttatatggg gcttttaatt ttagtctgta tggtgtataa agcaattaca
     5761 tgtagaaaaa aatcaacaca aagtcaggaa tagagccact tattaatagt ttttatttaa
     5821 agaaattttt tatataatcc gaatttaaaa agtcaaaata gttaaataaa ttgtaaaata
     5881 tgataatata tttaattaaa ataatatagt aaaatataaa tataaactaa aattaaattt
     5941 ataaaaa
//
LOCUS       DAYAOX010000222         5776 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.223, whole genome shotgun sequence.
ACCESSION   DAYAOX010000222 DAYAOX010000000
VERSION     DAYAOX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.223"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            57..2288
                     /gene="pflB"
                     /locus_tag="ACGI57_003191"
     CDS             57..2288
                     /gene="pflB"
                     /locus_tag="ACGI57_003191"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFG9718246.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPGALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            2448..3182
                     /gene="pflA"
                     /locus_tag="ACGI57_003192"
     CDS             2448..3182
                     /gene="pflA"
                     /locus_tag="ACGI57_003192"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFG9718247.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNIDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3304..4974)
                     /gene="pdcB"
                     /locus_tag="ACGI57_003193"
     CDS             complement(3304..4974)
                     /gene="pdcB"
                     /locus_tag="ACGI57_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase PdcB"
                     /protein_id="HFG9718248.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
BASE COUNT         2047 a          819 c          830 g         2080 t
ORIGIN      
        1 taaaaattaa ttttaatagt attatttatc atttaaatct aaggaggaaa tacttaatga
       61 atgcatggca aggatttaaa acaggaagat ggactaaaga aataaatgtt agagagttta
      121 tacaattaaa ctattctcca tatgagggaa atgactcttt cttagctggt gccactgaaa
      181 atactaaaaa attatgggac gaagcaatgg tcctatttaa aaaagaaaga gaaaatggtg
      241 gtactttaga tgttgataca aaaactgttt ctggaatagc tgcatatgct ccaggatatt
      301 tagataaaga attagaaaca atagttgggt tacaaacgga tgccccttta aaaagagctg
      361 tcatgccata tggtggtata aagatggttg aaaattcatg tgaagctttt ggatatgaat
      421 tagacccaga aataaaagat atatttacta aatacagaaa aacacacaac caaggagttt
      481 ttgatgttta tactccagaa atgagagctg ctagaaaatc tggtataata acaggtcttc
      541 cagatgctta tggtagaggt agaataattg gtgactacag aagagttgct ctatacggtg
      601 ttgatgcttt aatagaagat aaaaatgagc aaaagaaatc attagaagtt tcttgcatgg
      661 atgaggaagt tatcagatta agagaagaaa taactgagca aatatcagct ttaaatgaac
      721 ttaaaaaaat ggctgaatct tatggatttg atatatcaaa accagcaact aactcaaaag
      781 aagctgtaca atggttatac tttggttatt taggtgctgt taaagaccaa aatggtgctg
      841 caatgtcttt aggtagaaca tctactttct tagatatata ctttgaaaga gatttaaaag
      901 ctggaatcgt aacagaagaa gaattacaag agtatatgga ccattttgtt atgaaattaa
      961 gaatggttaa gttcctaaga actcctgatt acaataacct attctctgga gacccaactt
     1021 gggtaactga gtgtatagga ggtatgggaa tagatggtag aacattagtt actaaaaact
     1081 catttagaat gttaaatact ctatatacat taggaccttc tccagaacca aacttaacag
     1141 ttctatggtc aactaaatta cctcaaggat tcaaagattt ctgttctaaa gtatctatag
     1201 atacaagttc agtacagtat gaaaatgatg atttaatgag agcatactgg ggagatgact
     1261 atggtatagc ttgttgtgta tctgcaatga gaattggtaa acaaatgcag ttctttggag
     1321 ctagagttaa cttagctaaa actttattat acgctataaa tggtggtgta gatgagaaat
     1381 ctggtgttca agttggacca agatttgaac ctataacttc tgagtactta gattatgatg
     1441 aagttatgag taaatttgaa ccattcactg attggttagc aacattatat gtaaatactt
     1501 taaatgtaat acattatatg catgataaat attcttatga agcattagaa atggcattac
     1561 atgatagaga tatatttaga actatggctt gtggtatggc tgggttatca gtttgtgcag
     1621 actcattatc tgctataaaa catgctaagg ttaaaactat tagaaatgaa caaggaatag
     1681 ctgttgattt tgaaatagaa ggagattatc caaaatacgg aaataatgat gatagagttg
     1741 atagtatagc tgtagagtta gttgaaagct ttatgaataa aataagaaag aataaaactt
     1801 atagaaattc ttatccaact caatctatac ttacaataac ttcaaatgtt gtttatggta
     1861 agaaaactgg taatacacca gatggtagaa gagctggtgc tccatttgct cctggtgcta
     1921 acccaatgca tggtagagat actaatggtg ctttagcttc attatcttca gttgctaaat
     1981 taccatatga acatgctcaa gatggtatat ctaatacttt ctctatagta cctggtgctt
     2041 taggaaaaga tatgactgaa agaataaaca atctttctgc aatgatggat ggatacttcg
     2101 ctcaaaatgc tcatcactta aatgtaaatg tatttgatag agctacttta gaagatgcta
     2161 tggaacatcc agaagaatat ccacaattaa ctataagagt atctggatat gcagttaact
     2221 tcataaaatt aactaaagag caacaattag atgttataaa cagaacattc cacggtaaaa
     2281 tggcttagtc tttaaatttt tatactttaa ctaagattac tactgtgtaa aaaataaaaa
     2341 taaaaggcta tcttatattt gaattatttc tggtataaga tggccttttt tagagaaata
     2401 ttataactat tgataaggtt attaaaatat attgtgaggt gaactctatg attaagggaa
     2461 agatacattc tatagaaacg tttggtactg tagatggacc aggaataaga tatatattat
     2521 tttttcaggg gtgtcctctt agatgcaaat actgtcataa cagggataca tgggatatta
     2581 aatcaggaaa agaatacaca gttgatgaaa taataacgga tgctcttaag tatacttctt
     2641 ttatgaagtt ttctggtgga ggtataactg cctctggtgg tgaatccact cttcaaccag
     2701 aatttttaag tgaattattt aaaaaagcaa aagaaaatga tatacacact tgcttggata
     2761 catctggatt tgtagatata gaaactatag acccagtttt agataatatt gatttggttt
     2821 tacttgattt aaaacatatg gttgaggaaa aatctattga tttgactggt gttggtatgg
     2881 ataaggcatt aaaacttgct agacacttag aatcaagaaa tattcctgta tggataagac
     2941 atgttttagt tcctggcatt actgatgata tagataattt agaaaaacta ggtcaatttg
     3001 tagctacatt gaaaaatgta gaaagatttg aattgctacc ataccattct atgggaatac
     3061 acaaatggga aagtttaggt atcgattatg aactaaaaga tgttcctgat gccactaagg
     3121 aagatgtcca aaaagctagt gaaataatct ctaaatttgg agtgaaagta tataatagtt
     3181 agtgtagtat attaatcctt tatagaagac ataacaaaat agggtaaagt gtaaattttt
     3241 aataaacttc aaaacttcca ctttgcccta tttttatata ctatatatct cattttatat
     3301 acatcacttt gatagctgaa atttataaaa ttcagaggca cttacaggtc ttccaaataa
     3361 aaaaccctga ataatatcta cacctaagct ttttactata tcatactctt cataagactc
     3421 tattccctca acacaaactt ttaaatttat aatatgagct aatttaataa tatattcaag
     3481 aaaagtatat tcataactat tatatgtaat attcttaaca aaacttcttt caatttttat
     3541 tatattaaca gacagttctt taaggtaatt taaagatgaa tatcctgtac caaagtcatc
     3601 tattgcaata tatactccta taccctttaa actaataaat ttatcattta gtaaatttat
     3661 atctggtatc caacaattct ctgttaattc caaaattaaa aattcaggtc ttaattgata
     3721 ctctactaaa cattctacta taaaatctct aaaaaaatct tcctttaact gaatatacga
     3781 tacatttaca gatattttaa agtctggatt tattttatgc cattctttac actgttttac
     3841 agcttctttt ataatccact tcccaactgg tataattaaa ttactctgtt ctagtattgg
     3901 tataaattca acaggagata cttctccata agtactcgaa tgccatctta aaagtacctc
     3961 tgcccctatt acctcttttg taacagcatt tacctggggt tgaaaaaaca actcaaaatc
     4021 attaaaatta ttttcaacgc actctcttag tttttgttgc attgaaatta cctttagttt
     4081 attttcatag agctcttgtg aaaaaaactt aattctattt ttaccactaa tttttgctat
     4141 atccaatgca atatcagcat gtttaaataa atctaaataa ttatctccat cttctggata
     4201 catagcaacg cctgcagtca ctgtgcaata gtacttattt gattcaattt catgctgagt
     4261 atttgtatac atctgtatct tttcatatat tttctctata gtttctttag tacacatagg
     4321 gtaaaaaaat gcaaattcat ctccatctaa cctatacaat cttacatctt ttggaagata
     4381 ttctaatact tcagtagcaa ttgcacgcaa tactttgtca ccataagaat gtccatattt
     4441 ttcatttata tttttaaaat tatcaatatc cataataaac atagcaccat ttttataaat
     4501 tttctctttt ataagataat tcattctatg ttcaaattgt tctctattcc acgttcctgt
     4561 gatagaatca aatttatttt tttcaccaat attctttatt ctcccgacta gaaatatagt
     4621 ttttgggtca tccgacacat aaactttacc tctacaagaa atccatacta tttctttata
     4681 tttattaatc aacctatatt ctaaattgtg ctcacctttt tttccatgta gtaattcttg
     4741 tatatcatct ttccatattt gaacatcatc cggataaaca attttactcc aacaattgac
     4801 caaatcagat tctatctctt tacttaagtt aaattcatca aaaattgctg aagaaatttt
     4861 aaatttattc ctatttatat cccaaaaaaa tatataatct tctgtacttt gagataaaag
     4921 ttcaaagaaa atttctatat ggtctaaact taaagagttc gattcttctc tcatcttttc
     4981 cccctacaat attactatta gtgtagttta atcaacaaaa tatccttatt tagattatac
     5041 tattttacta attttttgtc tcatcaaaca catttcccca actttagtta tcttatcaaa
     5101 tctcttactc ttaactaagt tttttgagat tcataaagta atttttctat aatcattact
     5161 ggtgatttat ttttacctgc taaaacacca tgttaaatca tttattaaat cattttctct
     5221 taaaattcta tttttcgtta tataaagagt tatcagttct ttattctata aaacttttct
     5281 attactcttg aatttatttt atttttttct tcgcctattc tctttggatt tggtattgct
     5341 tttctataga ttttggcaaa gaagtgtctc ttcctataat ataagatttt gatatttctt
     5401 catcatctat aaatttttta atagggatta tatcattttt tatatttatt tatataaata
     5461 ttttttgttt ttaatttttt ctatcttctt atgtacttac atttaattta ttcttttcag
     5521 ttaataaaat aggtacattt ttagtacatg aaaatagagt tgcttacagg gtatatagga
     5581 ttgatgtact atttactaat attacatttt caactgattc aacccctctt ttacttacat
     5641 aatttaagta tatttttctg gatttttaaa tttatgtact ttaaaatgga tatacttatt
     5701 ttcattgatt ttgtaagaat taataaaaaa tacgattaca ctacaaaatt agaatgtcaa
     5761 tgtttataaa tataaa
//
LOCUS       DAYAOX010000223         5740 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.224, whole genome shotgun sequence.
ACCESSION   DAYAOX010000223 DAYAOX010000000
VERSION     DAYAOX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.224"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            127..627
                     /locus_tag="ACGI57_003194"
     CDS             127..627
                     /locus_tag="ACGI57_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFG9718249.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            684..1670
                     /locus_tag="ACGI57_003195"
     CDS             684..1670
                     /locus_tag="ACGI57_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9718250.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1720..2442
                     /locus_tag="ACGI57_003196"
     CDS             1720..2442
                     /locus_tag="ACGI57_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HFG9718251.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2471..4405
                     /locus_tag="ACGI57_003197"
     CDS             2471..4405
                     /locus_tag="ACGI57_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9718252.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4398..5666
                     /locus_tag="ACGI57_003198"
     CDS             4398..5666
                     /locus_tag="ACGI57_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HFG9718253.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
BASE COUNT         2412 a          431 c          978 g         1919 t
ORIGIN      
        1 attgggaaaa tagttcgcta aaataatttg tttttataaa attactgttt aagtttatag
       61 attttgtata aaataaagtt atgttattaa tttttttaaa aataaaactt ttactaggag
      121 acagatatga gaaaaaatag tattaatata gcactaactg cagcaattac agcaatctat
      181 gcagttttaa cattatcatt gggatttata agttatggac caatacaatt tagggtagca
      241 gagattatga tgcttttggc atttttagat aaaggatata tagtaggtct tacattaggt
      301 tgttttttag caaatgtaat aggaccatat ggtgtacctg atataatttt tggaacatta
      361 gccacattta taagtgcaag tatgatttat attactagaa agttaggtaa acaaaataaa
      421 agtactttag ttatagcttc tttatggcca actgttataa attcgattgt ggttggtcta
      481 atgttaaatg tattctttgg gcttccatta attctatcta tgatacaggt aggttttgga
      541 gaatttgtag tagtaacaat tgtaggagta ccatttttct gctttttaat gaaaaaatat
      601 aaaaatgtca ttaatattaa attttaaata gtgtatagta atttaattaa ttgagagtat
      661 tattagtgaa aagaggtgtt tatttgtcga aggtaaaaag attgactgat gtttggatat
      721 atattaatga taagcaaaag tttacaataa aagagttgtc agaggaattt aatctttcag
      781 ctaaaacaat acaacgatat ttaaaagaat taaataaaat gggacttcca attcaagcag
      841 aacaaggtag aaatggaggt tatagggtgc taaataatag ctatatacct cctgttatat
      901 ttacagaaaa agaagttatg attatactat ttgcacttaa gtcacttcag atttataatt
      961 ataagttaat ggaaatagaa attaattcta taatgagaaa agtaagttat gaacgtaaaa
     1021 gcagtataaa agtaggtata gaaaatatta aaagatacat tggctttgta gctaatgata
     1081 ctaatgatgg agattttaag agtagtaatg taactgagtt ttttagagct tcttcagaat
     1141 ctctaatttt aaatattaag tataagtgtg gtaatcagat tttggaaaaa aacgtatgcc
     1201 ctataggtat atatttaaat aagggagcat ggttttcacc agcatatgat tgtaaaagtg
     1261 ataaaataat attagttcat atagataata taattgatat aaaaagaaca ggagaaagta
     1321 aaaaaataca tataaacttg gaagaatggt tacatgaagt atataacaat ttatatggaa
     1381 atagttcaac tactaaaaaa tttggtgaaa ttatttatct aaatgtatta ttaactaaag
     1441 aaggtgtttc taaaataaag gacagttctt ggaatttaga tatggagcta aatgaagatg
     1501 gtagtggaat aattagtaca ttcataaagg ttgaagatat agattgttat gcttcgattt
     1561 tattttttat tggaaataat gctaaggtta tagagccaaa atttttgaat aagctacttt
     1621 atgataaggc caatgagtta agatattttt atgaagaaaa tatggtataa tatttagaaa
     1681 tgttgaaatt aaataattat taaggagata tacgttataa tggaaaattt taataagaat
     1741 gctaaaataa ttggtattgg agctgaaggt attaatataa taaatgaagt agaagaaaaa
     1801 ataaaagcaa atatggatat agaaaaaata aacataaatc aagagattga aaaagaatat
     1861 gttagaagtc ttttagatgg tgtagatata ttatttttga tatattcatc tgaagataaa
     1921 catattagag atattataaa agctgtttca tatatgtcaa acgaaagaag agttctatct
     1981 ataggaatgg attgttcaga gaaggaaaat aaagaagatt tagagcttgg gagagagttt
     2041 aaaatcaata atgatagtat atttaaattt gttgatttaa tgaatatcat ggtagagtct
     2101 atttctgatt cttgcatgat aaatatagat attactgatt taaaagaagc tattgtagga
     2161 gataaaggaa ttaagtattc ttttgaagag tttgaagata caaagtcata tagcgagata
     2221 gctgacattt tatttgacag gatggaatat attggtgagg aatttataag taagaaagga
     2281 attgtatttg ttgaaggaag tccagaattt tctataatgg aattgaatga tttaataagt
     2341 aatattcaga gcaaggtaga agaaagctat gaagtaatat tttctttata tataaaggaa
     2401 aatttaaatg gaaatattag agtaggctta ttgtataatt agtatatttt agatattaag
     2461 gagagtgtat atgtctgaaa atataagagg tacagagtat tggttaatgg aagaattttt
     2521 ttatcaggaa acagacacac cacagatttt tagagaagaa agcataattg cacttggatt
     2581 tgatataaaa ataaatttaa aaaaagtatt cagtatgggg aaaaaatcag aaaagtatat
     2641 taatgagaat tgtaatgata aagattttat aaaaaagttt atgtcgatta aaaaaggaga
     2701 ttatttttta tataaagcat atcaaaatat agaagaaaaa gttaaatgta atgtgtctat
     2761 tggcatagtt gaagaagatt ttgaaaatgg atatgaatta agtcatattt tagggcatac
     2821 actaccagta aaatggatgc ataattttga tacagtttca aattgtaaag tatatacaag
     2881 tgaattttat aaattaaata ataatgatat tagttcatat ttagaaatta ttaagtcaca
     2941 agataagaaa ttattgagaa aaagaataga agaaagtgaa aataataaat ggtatttaaa
     3001 aaactattat tataaaaatg tagataagtg tgtagatgat gaagaattat ttattcagcc
     3061 cattaatcta gaacaattag aaagcctaaa aaatataaag acagatgatt atattttatt
     3121 gtataatagc gagaaagtaa tttgtgaaaa tgaccataca atgaagagtc tatttggcgt
     3181 atgtcaagtt atggaagggt tcgataaaga gaaagtaagt gctttggagg gtggatattt
     3241 ttttcctgtt agatggatag agtatgaaaa ggagaatata gacggaaatg catgggttat
     3301 atttgaaaat aatagtatag ttgaaaagta tgtaaaaaga tttactaata aaatgccatt
     3361 aaatactata ctttatggac caccaggaac tggtaaaaca tacaatacaa aacagatggt
     3421 ttacaatcta gtaagtgaaa ccagtctaag aaacagatac tatacaggtt acttagatta
     3481 taaaaataat gtagaatttt gtacatttca tcaatcatat ggatatgagg aatttataga
     3541 gggccttaag tctgatggtg aaggcaattt taagccagaa gatgggatat taaaggatat
     3601 atctatagaa gcatgttata atgcattgaa atttgaaaaa aagatgagct ttgagttgga
     3661 aaaagatagc ttgactttag aaactataaa agacaggaaa aagaaattag tattagaaaa
     3721 tatagaatat agtgagagtt ttaattttga agaatcagaa aactatgttt taataataga
     3781 tgaaattaat agagggaatg tatctaaaat atttggtgag ctaataacat tattggaaga
     3841 tgataaaaga cttactaaat caaatcaaac tattgtcaaa ctaccatatt caaaggaaaa
     3901 attttctcta cctccaaatt tgtatatagt tggaactatg aacacatcag ataaatcaat
     3961 agcactttta gatatagctt taagaagaag gtttaatttt gaagaaatta tgccagatta
     4021 tgatttattg tctattgtag atggtataga tattagaaaa atgctatata caatcaataa
     4081 aaggatagag tttttatatg atagagacca tgttattgga caagcttatc tacttgctgt
     4141 agaaaatatt aatgatatag tagtagtatt tagaagtaaa ataataccac ttcttcaaga
     4201 gtatttttat tatgattcag aaaaagtagg tttgatttta ggaggaattg gaagaaatga
     4261 aaaagataaa tacattgttt ataaagaaaa acttatagct tctgagttat ttaaaaataa
     4321 taatgcctca ataatggaga gtaaagttaa ctattgtata aaacaaaaca tatctagtga
     4381 ggagctaaaa aatatatatg agtaaaaata tatatgttaa ggaaacctat gaatggataa
     4441 ggataggtaa tggtgaaaat gagcttacag aaattgaata tgaaaaattg cttaagtatc
     4501 tagagaacaa taatgatgta ttaaaaagta atataataga tattaaatac aaaaaactta
     4561 gatttataaa ctatgtaggc atcatatgtt ttgaaaatgt aatacttgaa atacttccta
     4621 aattgtcatt aagtgataat cttgtaaaag atagagagat actattacag atgctttcta
     4681 tatgtaataa aattccaatt acaatgaatg aaaaaataag attgagtttg aaaaattata
     4741 atctcttaaa tttttttgtt atgtatttca ttgagagtat gcaaacacag atgaaaaaag
     4801 gtatttattt tgaatatata aataaaatag agaacttaaa tgttatgagg ggtaaaatat
     4861 tattaagtac atatgcaaaa gaaaaaggca tttctccaat gaaaataaga tgtgaatatg
     4921 atgagtatag tgaaaataat ttcttgaatc aagttcttaa aaaagcatgt atatctattt
     4981 tatgtagaat aaatgacaat tcaattcaag gcaagattaa aaaaatttta agctattttc
     5041 aaaatgtaga tttaatttat atagatagaa aaaaactgtt ggattataaa ttttataaaa
     5101 acaatgatag atttaaagat tgttatttac tagctagact tctactttta aatttgtcta
     5161 tggataatag tcaaaataat caagaggcat tttctatatt atttgaaata aatactttgt
     5221 atgaagaata tataggaatt ttaatcaaaa gtatatggga caattcattt agagaaacat
     5281 atatacaaga taagagtaaa tttcttttga aaaatgaaca aactggtaag aaaaacttta
     5341 atttaagacc ggatatagtt ttatatgact taaaaaatga atatgaaatt ataattgaca
     5401 ctaaatggaa agctattgaa gtagattcaa atgtttttta taggtcaagt gatatatacc
     5461 agatgtatgc atatataaca gcatatgaaa atgcaaaaag atgtatatta ttatatcctt
     5521 gtattcaaaa agataagaat tatagttcct ggaaattatc agaatcattt aagggtaagt
     5581 ttatagaagc aaaaactgtg aggttagatg atataaaaaa cacaaaaaat gatttaaaaa
     5641 agataatttt taattataaa ttttaaaaat aagaatttta aaatttataa aagtaatttt
     5701 atataggtat aaaaattacg agtagtataa aaaatttaat
//
LOCUS       DAYAOX010000224         5740 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.225, whole genome shotgun sequence.
ACCESSION   DAYAOX010000224 DAYAOX010000000
VERSION     DAYAOX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.225"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            393..1598
                     /locus_tag="ACGI57_003199"
     CDS             393..1598
                     /locus_tag="ACGI57_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9718254.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1669..1890)
                     /locus_tag="ACGI57_003200"
     CDS             complement(1669..1890)
                     /locus_tag="ACGI57_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718255.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2031..2630)
                     /gene="recR"
                     /locus_tag="ACGI57_003201"
     CDS             complement(2031..2630)
                     /gene="recR"
                     /locus_tag="ACGI57_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFG9718256.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2643..2993)
                     /locus_tag="ACGI57_003202"
     CDS             complement(2643..2993)
                     /locus_tag="ACGI57_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFG9718257.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="ACGI57_003203"
     CDS             complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="ACGI57_003203"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFG9718258.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4766..5030)
                     /gene="ffs"
                     /locus_tag="ACGI57_003204"
     ncRNA           complement(4766..5030)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACGI57_003204"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5068..5159)
                     /locus_tag="ACGI57_003205"
     tRNA            complement(5068..5159)
                     /locus_tag="ACGI57_003205"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5123..5125),aa:Ser,seq:gga)
     gene            complement(5160..5615)
                     /locus_tag="ACGI57_003206"
     CDS             complement(5160..5615)
                     /locus_tag="ACGI57_003206"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HFG9718259.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
BASE COUNT         1826 a         1006 c          644 g         2264 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata tcatctttat ctattctagt caatataatt
       61 ttttactttt tttatttttt ttatttatca tgtattcttt atatttaatt tttaaaactt
      121 aatgctaagt aaacctgttt atttcataaa actaatatat attctatgct ctattttcct
      181 ttttattcta tatttaaatt ttttatttac aagaatattt ttaatataac atatttgata
      241 aaattaattt acatttcttt cacttgtaat ttttgcaatc ttgtattaga ttaatattaa
      301 aaataattta tcaataacct tattcacaaa gacagttttt atatatcaaa atataatata
      361 aatactatgc tacattttag gaggatttta ttatgagcaa aaaagtatta ataatgtctg
      421 cttccactgg tggtgggcat aatagggctg ctctagctat taaagaagaa cttacttcca
      481 aaacacttga tggagagcca attgaatgtg aaataataga tagtttgaaa ttagttaata
      541 atacaatgga taaaataatt tcaagaggat acgaaaaatc tgctatatat actcccaaag
      601 cctatggaag tgtatataga ttatctgaaa caaatctttt atcaaaaaat gaatttaaag
      661 ataatctact aataacattc atggctaaaa aatttaaaaa acttattaga agcgaaaaac
      721 cagacttgat tataggaacg catccttttc caatgattgc tctaagtaca ttgaaaaaaa
      781 actttaattt acataataat gaatcaaatg cttacacaga gcatttttat aaacattata
      841 caaataccat taatgttcct ccgcttattt ctgtattaac cgattatact actcattcta
      901 catggataca aaatgaaatt gattactata ttgttggtca tgaatatgta aaagaattat
      961 tggtgtttga tggagttgaa ccgagtaaaa ttagaacatt tggaatccct gttgaaaaat
     1021 cttttctctc tcacagagat aaaggtattg ttctttctga gttgaattta tctcctgata
     1081 aattaactgt tttacttatg gggggtagct ttggggctgg aaatataaaa gagacacttg
     1141 atgaattact ggatactgat agagactttc aaatacttgt aattactggt aagaatgaaa
     1201 gcctaaaaga aaaaatcgaa aaaaaactta tgtctcgtta tcatgataaa aatgtatgtg
     1261 ttcttggata cactgataag atgaatgata ttttagcttc tatagatgta ttagtaagca
     1321 aacctggtgg acttactaca actgaggctc ttttaaaaga tgtacctatg atagttcctt
     1381 attatatacc aggtcaggaa gaagagaatc ttgatttctt atctaattgt ggtgcagcac
     1441 ttagaactac aaaaaaatat aacttgtcaa tattactaaa agttttaata gatgacccta
     1501 gcagattaga aatgctaaaa agaaatatta aatcaataag aaagtcaaat tcagcccaaa
     1561 atatagctaa tttaatttta aatataattt ctggttgata aaataaataa aatccctata
     1621 gtatatatat tatctattag ggatttttta ttttctaatt atttaatatt aaattggttc
     1681 ataaaagtct tcttcataat catactgata atttttataa ttatttctaa attcagcatt
     1741 tttatttcgt acattttttt tagttttttt cttattagaa aaacttatat cctcaaaatc
     1801 atcatattta ggtttatctt ttgatttcat ttttttaact cttttttctt tcattgcatt
     1861 atgttcttct aagttcataa ttggtttcat gttgcacccc tatcaaaatt tttaaatgat
     1921 ttatacaaca aattatacaa tacagaagaa atttattcaa taaatctaaa tcaaaattaa
     1981 tttgaattta actcaaaaaa ttacaaaaat cgaggtatta acctcgattt ttatatttct
     2041 cttctgcctt ctagtgcttt tgatattgtg acctcatctg catactctaa atcgcctcca
     2101 acaggaagac catgagctat ccgagtgact ttgattccca ttggttttac taacctagca
     2161 atatacatgg ctgtagcttc accttcaata gtaggatttg ttgccattat tatttctttg
     2221 acttcctgat tgccaagtct ttggataagt tcctttattt ttaacatatc tggtccaatt
     2281 ccatccattg gagaaattac tccatttaat acatggtact gtcctttaaa ctctctcgtt
     2341 ctctccatag ctgctacatc tcttggatct tccacaacac atataacact tgaatctcta
     2401 cttttatttg aacacatgct acatgggtct gtatctgtta tattacaaca aattgagcaa
     2461 tatcttattt ctctttttgc atctataatc gctttagata aagcttccac atcattagta
     2521 ttcatattaa taatatgaaa agccaatctt tgagcagtct ttcttcctac tccaggaagc
     2581 tttgaaaatt cctctataag tctcgttatg ggacctgtat aaacttgcat aaataccacc
     2641 tactagaata acccaggtat attcatacct ccagttacct tactcatttg acttgcttgt
     2701 atatcatcaa tatttctcaa tgcttgattt actgcactta aaactaaatc ttgtagcatt
     2761 tctatatcat ctggatcaac tacttctggc ttgatagtta tatctataac ttctttttta
     2821 ccattaactt ttacagtaac tgctcctcca ccaactgaag cttctacttc tttactttct
     2881 aactcttgtt gagctttttg catattttct tgcatttttt gagcctgctt taacaagttg
     2941 ttcatatttc caccaccagg catcattccg cctccaaaac cttttttagc cataaacact
     3001 attcctccta aacttatttt cattttatta ttatatcata atagtatttt attatttact
     3061 ctcattttca tcaatactat cttttatttc taatatctct tcactcacta tgttctttaa
     3121 tatttcctca ccaatgtcct ttttctcttt aacttcaaga tttatatctt taacttctga
     3181 tgtcagtact atttttacat taaaacttct atttaaaaca tctcttattg ttttttctaa
     3241 ataatttatt gtatcttgag agctcagtct agtttttgca aaatcaaatt tatcatcaaa
     3301 tataacatac aacatattag aatatacatt aaaacttttt acttcactta agagtgcgta
     3361 tattggcatt tttttatctt ttttaatttg tttaagtata ttcttccaag atgactctat
     3421 aagtataacg tcctcatttt ttacatcttc ataaactaca ttttcttgtt ttacatctat
     3481 ttctctatcg acctcatact ctttttcttt attattccca attttttcag acttaaagtt
     3541 ccccagttca atcatttttt ctaagttctc aactcttttt ataagagctt ctttactttc
     3601 atcaaacatt ggttgagcaa ttttcattat ggttatttcc actaaaaccc ttggatttga
     3661 agatgacttt atatcatcct gagttattga taatatattt aaaatcctta ttaaatcatt
     3721 tatatctata tttttagact gaattttaag ttgttctata gtttcttctg gtaatgaaat
     3781 tatttcatcc aattctgatg aaaccttaca taccattaaa tttctaaaat gatcaattaa
     3841 gtcatttata agatttctta tatctttgcc ccataagaca aattcattta atatctcaag
     3901 agacttttta gtgtcctgct ctactataca ttgagacaat tcaaataatt gttctatatt
     3961 tacgcttccc attaattcaa caacatcttt ataatctatc ttactttctc caaaagacat
     4021 acattggtct aatatactaa gtgcatctct aagagctcct tgagaatttc tagctattaa
     4081 attaagagct ttatcatcta tatcaattcc ttcatcttca caaatctttt tcattctaag
     4141 ggtcatatct ttaactgtaa ctcttttaaa atcgtatctt tggcatcttg ataaaatggt
     4201 tgcaggtatt ttgtgaggct ctgtagttgc taatataaaa attacatatg atggaggttc
     4261 ctctaaggtc tttaataatg cattaaaagc tccttgagac aacatatgaa cttcatctat
     4321 tatatatact ttatatttag cattggctgg agaatatttc acactttctc taagctctct
     4381 tatatcatct acactattat ttgaagctgc atctatttct acaacatcca ttgtattatc
     4441 ttttaataca gattcgcata tctcacactc attacacggt tcttcattta ttttattcaa
     4501 acagtttact gctcttgaaa aaatctttgc agtagatgtt ttaccagtac ctcttgttcc
     4561 acaaaacaaa taagcatgtc ctatattatc actatatatt tgattcttta aagtcttaat
     4621 aatatgatct tgaccaataa catcttcaaa tttttgtggt ctatatgctc tatataatgc
     4681 tttatgcatc tttttacctc tctttagcaa aattaataaa aaaatccctc attatctaag
     4741 ggattagtct tttatatatt aaatgctgta caccatctat cgacttaatt ccataggcgt
     4801 taccaaaaca gttagctcaa cttaggcacc cccacggcac acaaaagtga tcacttaccg
     4861 ctgcttcctt ccggatctga cggggttcat gagctttcat tgcgcaggac ctaagcatca
     4921 acaccactta tttagggcgg ccctacagaa aataagcctt tagatggaat tcaatcctgc
     4981 tatagcggat tgcaggttac agggcaccgc tacctcccca tctagtacag caaaattaca
     5041 accatattta attactttca ttgtttctgg cggagagagt gggatttgaa cccacgatac
     5101 agttttaccc atatactcgc tttccaggcg agcgccttcg accactcgac catctctcct
     5161 taatctcttt ttctcaatgc cttaaaaaat tcttgcaata tgatcgagca ttcttcttgc
     5221 aaaacatcaa aagctacttt tacattatta ttattaaaat aatctaattt aaactcatgc
     5281 tgtttttcta tatatgaatt ttttacatgt cttgtcccta ttataagctt ttttattcta
     5341 gaattaacta ttgcgccact acacattatg caaggttcca ttgtaacata taaatcacaa
     5401 tcagtcaacc tccaaccacc taatttttct gatgcttgtt ttatcgctaa aatttctgca
     5461 tgtgccgtag agtcttttaa cgtttctgta aggttatgag ctctagctat tatttgatta
     5521 tctttaacta taatagcacc tattggtgtt tcttttttat tatatgcttt atatgcttct
     5581 tttaatgctt ctttcatata aaatgaactt tccataattc ctcccaaaac ttataacaaa
     5641 cttatcttat cataattcat tttataattc aacttaattt tattttgttt atttaattat
     5701 tactatatat agctatattt ttttaaaatt agttctaaaa
//
LOCUS       DAYAOX010000225         5735 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.226, whole genome shotgun sequence.
ACCESSION   DAYAOX010000225 DAYAOX010000000
VERSION     DAYAOX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.226"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(157..1890)
                     /locus_tag="ACGI57_003207"
     CDS             complement(157..1890)
                     /locus_tag="ACGI57_003207"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9718260.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     gene            complement(2130..2975)
                     /gene="dccA"
                     /locus_tag="ACGI57_003208"
     CDS             complement(2130..2975)
                     /gene="dccA"
                     /locus_tag="ACGI57_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP diguanylate cyclase DccA"
                     /protein_id="HFG9718261.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            complement(3006..5240)
                     /locus_tag="ACGI57_003209"
     CDS             complement(3006..5240)
                     /locus_tag="ACGI57_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9718262.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
BASE COUNT         1935 a          809 c          650 g         2341 t
ORIGIN      
        1 atttgctctt tagaaaaatg agcagtatca ataaatatat tgtttaaatt ttttctagga
       61 actagttgac tagctataag attagcttca actctttgat tgttagacta tgaaattaaa
      121 tttaaaggtt cattcttggt cgtataaata gctttattat tcgtatgtac tataacaatt
      181 tttgccatct gcttttgata gataaagagc tttatcagct ttagaaaata aatctttata
      241 taatttagtt gaatcatcag tgaaggcaat accaatactt aatgttattt tatgattgtc
      301 ctttactttt attttactcg catcatttaa aatatcatta gtaattttta ttattaaatc
      361 ctttgttaat gattttataa atattgtaaa ttcatctcca ccaattcgag caactatgtc
      421 tttatcatgt gtgttatgta atagtatttt tgatacagca actaaaactt catttccctc
      481 tagatgccca aaagtatcat ttacagtttt aaaatcatca atatcaataa ctaatagagc
      541 atcattattt ttggaatcag aactacatag atactcttca ataagatttt gtgatactct
      601 ttgattatat aattgtgtta gtgggtcacg ttgagcctga ttaattaaac tttctaattt
      661 agatttatct tcagttatgt cttttaaaac gcctattgtt ttgatagggt tatggtcact
      721 atcatatata actgaacatt gaacacgaat ccatctccat tcatttttag ttaatgaatt
      781 tacttgtctt atctcaattg tttcacgaag atttccatga agtacttcta gtagttttcc
      841 aatatcatca aggtgaataa ttttaccaga ttctaatagt ttggagtaat tttttgtttc
      901 aaaattttct aattcaattt ttttgtcaat ttctacacgt tgatatttta taagggtatc
      961 gttttcaata tcatactcaa acatgatatc tgtaatattt tgcaaagcaa tacgatatcg
     1021 ctctctttct aattttagtt cagattctat gttttttagt tgggttatgt cagtaataat
     1081 actattaatt tttactatgc catcactatt taacgatttc attccagaat ctaataccca
     1141 cattaatgtt ccatcttttt ttcggattct atattcagat gaatatgtag gtccttttgc
     1201 aaaacatcgt tcacaatctt ctagtgcttt aggtaagtct ggtggatata cagcacccac
     1261 tgctgaacca ccactcattt ccatgaactc atcatatgtg tatcctaata ttttgggcaa
     1321 tccctcattg acatagaaaa atgagtatgt actatcatca ttgctacctt ttaagccacc
     1381 attgatatta tttgtaatca tttcaataac ttctgttttt tcttttagta atctttgcaa
     1441 ataatcataa ctttgagttc caattttagt gggaaagtat tcatcaagtt gcatttcacc
     1501 ataagggttt gatatgtgca tgtgctttat taataatttt cctctatcct caattatctc
     1561 aaatgtacaa cgttgattta cctctaaaat catttgagca ttttctttag ttcgaacagt
     1621 atatcttcct aataccatac atctattctc atcttcactt acagtttcaa atatatcatt
     1681 atttatatca caagaaggaa tttcatcttg acctgcataa aaataatttt ttatttcatt
     1741 aaaagaagtg agaaattcct gttctcctgg tccaatccat gtaacatttt cacaaaggta
     1801 ttttaatact tcattgatat ctcgatttat ataaaaactt gttataatat gttttgctaa
     1861 tttgggtgct ttttttagga gtatgctcat gatattcctc ctttcttttt gctattatat
     1921 tttaattata ttatacatca atatatttaa attaaaagct ttgttaaact atgatatctt
     1981 tgttaaataa gttaatttta acaaaaaaga actgaaacaa tatatgcttc agtacttaat
     2041 gtcaatatct tgtatagata catatttata aactaatgta caagtttaaa aaaagaaaga
     2101 taattttaat ttattttaaa ataacaatgt taataatcat ttttatcaaa ttttttcttg
     2161 tttttctcct tgtttttata cattttttta tccaattcaa ctattgtcaa ttttaaattt
     2221 ctttttactg tatcaacacc ataactaaat gaaagatact tatacttaga gaatactttt
     2281 aaaaaatttt ctcttaaggc ttctagctgt gaataaacat aatttttgtc aacatcacta
     2341 gagaatataa caataaattc atctcctcct aatcgtataa tagaagtaga atttattaat
     2401 tctttattta aaagtgaaac aaaatcacat aaaatcttgt ctcctacatt atgaccttca
     2461 tagtcattta tcattttgaa attatctaag tcaattagta caatataatc tcctataaat
     2521 atgtcaaatc tatcatttaa atattttcta ttatatgctc cagttaatgg gtcagtaatt
     2581 gagtagtgaa gatttctctt attttcttca tagagttttg ttatatcatt ggccatagta
     2641 attatgtagg ttttttcatc aacatctact ttgtattcta aaacattcca atagcttgta
     2701 gtatcttttt taaactctaa aatccaattt gaaattttat cataaggata atttttttcc
     2761 caatctaatt gaatattgtg agaatccgta aaaaatttag caatattatc agggagtttc
     2821 ttagctattt ctatattagt cattccaagt ggattatctt gcatataact tttaattaaa
     2881 ttttttaaat tatcatttat aaatataatt ctgtagttag aatctcttat aattagaaat
     2941 cctggaaaag ttcttaaaaa aatttcttta aacatattaa tttctccaaa ataaaatact
     3001 ttgtactaga tttttgttaa attttttatt ttatttacat ctacatacat accctgggca
     3061 atcgaccttc catcaattgc agaaaggtta cattgtgtat acataactgt gacaatttga
     3121 ccatctcgtt ttatgaatct atataaaaat ggttcaattt tatttgtgtc aatcatgtcc
     3181 aaactttttt tataaacaaa gtctaaatca tctggatgaa caattgctct taaatttccg
     3241 cccatctcac caaaaaactg ttcttttgta taacctataa tatcaaacat ttcttcattc
     3301 atataagtaa taatcatatc accaaataaa tcgtagcgaa ttatacctac aggcaacatt
     3361 gaataaaatt gtgataaaat attactttca tcaagatgtg tatttgtcat tttaggtatt
     3421 gaatatgatt caaattcatt tatatagcca gcagttttaa ttaaatccaa atgtaatttt
     3481 gctaagagta atttattttc tgaaattata caatttgcac taaagtagaa attgctatat
     3541 ttttgatgtt cagaagcttc atctgataaa acggcttctc cactaattaa taatgaatct
     3601 gattcagtat tatgtggggt tatacttttg taaagaatag aaaatttttt acctttaata
     3661 tgtttttcaa aatgctctat tactttagga aaagatgtaa tttcagtttt gttgtaaaaa
     3721 tcaaaccatt gaatgtcttc agtgacatta gctcttagtt ttttcatatc ttgcataata
     3781 aaaaatccat caaatatctt ctctattaaa gattttccta tatcagaata ttttataaac
     3841 attttctctg aattattctc tttagatagt aatttttcaa attctttgca ctccatgggt
     3901 tttgcataat aataaccttg ggcgatatca caatttatac ttttaataaa ttctacatgt
     3961 tgttctgttt caacaccttc acatactgta agcatcccaa gagctcttgc aatgtttatt
     4021 atagagtaca aaatggttgt atttttaaaa tcatttaaat tatgagataa aaatatccta
     4081 tcaattttga ttatatcaaa aggtagattt tgcaacatac caaaagatga atatccagaa
     4141 ccaaaatcgt ccattgatac agaaaaacca taagaacgaa gttcagctag ttgttgaata
     4201 attgtatttg tattttctat taatatattt tctgttattt caatgctaaa atttgatgta
     4261 gagacgctgt acttttcagt aatacctttt agcatacttg gaaaattagg gttaaaaaaa
     4321 tgaagacgag aaaagttaca acaaattgga acaaaaacat cattgttatc aatccatctt
     4381 cttatatttt tacagttctc ttcaaataaa tgcaaatcta attccgttat cagaccaaac
     4441 tgttcaaata aaggaataaa tttatctgga gtaagaagtc ctttaatagg gtgtctccat
     4501 cttgcaagtg cctcacatcc aactatagta ttatttttta tattataaag tggttggtaa
     4561 aatacttcaa tttgtttagt ttgtagaaca tgtgatattt ctgataagaa ctccttatca
     4621 ttttctaatt tattgtaaaa actattatct ttaaaatcaa atagaatgta tctattttta
     4681 ccttcacttt tagccttttt taaagcacta tacacttggt tgaaaagcat caaagtagtc
     4741 atgtttggtt caccattcca gatagtgctg ccgatactaa atgtaacatt ggagtatatt
     4801 tctttttcat taggaagtct tatttttaat tctcgggcgc tctctataat agttgcaccc
     4861 atattattta actctttatt tgatacattt ggaagtacaa ttaaaaaacc ttctcctttt
     4921 aaataagcga ctattccctt tgattctgga ataaagtgca ataggttttg agttagagta
     4981 attaataaat tttctccgac taggtatcca tatatatcat taaataattt aaagttatcg
     5041 atatttaata acatgattcc tatatgtata tttttcgaaa tacagtcatc aatatgttgg
     5101 tacataaatt ctctgtttgg tattttagaa aaaaaatcta tttcatttaa ggtttttaat
     5161 ttaaaatttc tactatctaa ttccttagat aatacttcta cctttttatt taacattttt
     5221 atataattat gattttgcat ggtattcctc cataagatac tttaaatttt gatgacaact
     5281 gtatttatgc tagaagtatt taaatgttaa tttgtaaata atttaaatgc taaattttaa
     5341 ataagtattt aaaatgaata tacattaagt tatttgctcc aatgctacaa atacaaatat
     5401 tttttagttt agtaataata ttagataaaa ttagttaatg cttaaattct agtatattga
     5461 aagatttcct ttttgaatgc aattatatca tattttttca taaatagata gattgtgagc
     5521 tgtatagtta gtaaggtaga gtttttttat aattaatttt aagtttaatg atttttcaaa
     5581 ggcataatta ttttattcat aattaacttt ttatacataa tttaatacca tattaataat
     5641 tttgcataat taattttagt tattaaaatt aagtttattc ttgtattttt tgttaaagac
     5701 tataataaat attcatttac taaatttata aacta
//
LOCUS       DAYAOX010000226         5632 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.227, whole genome shotgun sequence.
ACCESSION   DAYAOX010000226 DAYAOX010000000
VERSION     DAYAOX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.227"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            352..1188
                     /locus_tag="ACGI57_003210"
     CDS             352..1188
                     /locus_tag="ACGI57_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9718263.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1541..3118
                     /locus_tag="ACGI57_003211"
     CDS             1541..3118
                     /locus_tag="ACGI57_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9718264.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            3111..4295
                     /locus_tag="ACGI57_003212"
     CDS             3111..4295
                     /locus_tag="ACGI57_003212"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9718265.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            4313..4912
                     /locus_tag="ACGI57_003213"
     CDS             4313..4912
                     /locus_tag="ACGI57_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9718266.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4945..5406
                     /locus_tag="ACGI57_003214"
     CDS             4945..5406
                     /locus_tag="ACGI57_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFG9718267.1"
                     /translation="MFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTGDIV
                     YAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVKEGT
                     PMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2139 a          576 c         1055 g         1862 t
ORIGIN      
        1 tatttgagaa aagattagta caaagctata atctgaattt caataaatat atatataatt
       61 aagttgaaac acttgaaaat actggaaaaa attgaaaata gtttttgtaa tataaaatgt
      121 cgattgattt taaataatta aatatacata agaaataatt aaatatattt caatgaaaat
      181 atgataaaat atgacaaatt aattgatgac aataaaaaat attttgttat tgtagtatta
      241 tagggtaaat tgcaaaatat atacgaaagt aaaattttaa atattgacat taaattaaat
      301 atactatact atgtaagtgt gatgagagaa tttttttaga ggggatttat agtgaatata
      361 aaaaagatat ttaataacaa tgtagtagta agctccctag aagatggcac agaaataatt
      421 gtcactggtg ctggagtagg atttaagaaa aaagtaggcg atttgataga tgaaaatttg
      481 atttctaaga aatattttgt tcaagacgat caaagggaca aatacaatca aatacttaat
      541 aagacctcta ttgaatactt taaaatttca gaagaaatta tagaaaaggc taatgaagtt
      601 ttaaatactc aagtgaatga ttcaataata ttagccttaa ctagtcacat agaatttgct
      661 gtccaaagag aaaaacaagg tataaaatta ccaaacttaa tattgaatga gacaaaacaa
      721 ctttataggg aagaatttga atttggatta tgggcaattg atgaaattga aaaaaggata
      781 ggaataaaat tgcctgaaga tgaagcagga tatattgcta ttcacataat caatggtttg
      841 gaaaattctc aaaaagatga aggtattaat atactagagt tttcaaaaca agttattcag
      901 ataattgaag aagtacatgg atttaaattg gatgtaaatt cattaaatta tactagactt
      961 ataactcact taaagttttt tgttcaaagg attctccgaa aagaaactta caatgatagt
     1021 gatattgaag atatgtataa gttagtgaat aaaaattaca ataaacctaa agtgtgtact
     1081 gaagaaattg cagcattggt attagacaga tttgaatata agattagtaa agaagaagaa
     1141 ttatatttaa tgattcacat aaataaaata acaaacagtg gttgttagga ttgtaactga
     1201 taaagcaggc aaaacctaag tcattgagct taactattag attaagtttt ttgatttagg
     1261 tttttttatt agatacaatc aaaatacaga cagtgacttt taggattgta actggttaag
     1321 caggcaaaac ctaaatcatt gaatttaact attaggttaa gttttttgat ttaggttttt
     1381 ttattagata caatcaaata cagacagtgg ttgttaggat tgtaactggt tgagcaggca
     1441 aaacctaaat cattaaactt aattattaaa ttaagttttt tgatttaggt ttttctataa
     1501 atttgattat gtttttagat aaaaatagga ggggaaaatt atgaaaaaaa ataagttagg
     1561 tttatggaac ttcttccaaa tgttaggaaa aacgtttatg ttcccaatag cactacttag
     1621 tgtttctggg atgatgctag gacttggggc aggttttaca gaccctaaga tgattgaaat
     1681 ggttccattt ttagggaatg aatatgttaa cttagtttta aacttcatgt taacaatagg
     1741 attatttgca tttaacaact tgggagcatt atttgcaatg gcaattccac taggattatt
     1801 aaaaaaagaa aaagagtttg gtgctttttc aggattagta ggatttatag caatgcatat
     1861 aggtacaaat ttttatttaa caagagctga tttgttagta ccagcagaac agatgtcaac
     1921 taatggtcaa gctatgatta tgggtataca aacatataat acaagtgttt taggtggtat
     1981 tgtagcagga ttaattgttt atgcaatata tgataaagtt agtaatttaa agataccaga
     2041 atcattagga ttctacagtg gaccaagatt agttccaatt gttagtttaa tagttatgag
     2101 tatagttgga ttattaatac ctattgtttg gccaccattt ttcaatgcat tccaaagttt
     2161 aggaagatgg atttcttcag caggaccatt aggatatttt tcatatgcag tggcagaaag
     2221 agttacaatt ccatttggac taaatcatct tgtaacatct gtattccgtt ttacaccaat
     2281 aggtggaact gctgttattg atggagaaac gtactttggt acattgaaca tgtttatggc
     2341 atatgtagaa aataatcaga ttatacctct tgatttagct ggtaaaatgg aacaaggtaa
     2401 attgatgatt caatatggat tagctggtgc tgcattggct atctaccgta cagcaaaacc
     2461 agaaaataga aaggcactta aaggactttt aatatcagga gtgttgacag ttattatagg
     2521 tggtataagt gaaccaatag agtttctatt cttattcata tcaccagcat tatttgcttt
     2581 ccatacattt atgaatggtc tagcaaacat ggtacttcca tacttaggcg tgttgatggg
     2641 attcacagga gatttaattg catttataag ttttggtgtg ttaagaggaa cagctacagg
     2701 atggccaata gcagtattag tagcagtttt atattttgca atatactatt ttgtatttaa
     2761 gtgggcaatt attaagttta atataaagac cccaggacgt gaggataaag aaataaaagt
     2821 atctgaagga aatggttcaa atttcaaaaa tttgtctaca tataaaggac aacaaatgat
     2881 atctgcttta ggtggtcaat ctaatatatt atcactagat aattgtgtaa ctagattacg
     2941 tttaaaatta caagatgtat cattaataaa tgaggatgca attaaagagg ctggtggtat
     3001 agcagttgtt aaattggatg agcatacaat acaagtaatt ataggaaccc aagtgtattc
     3061 tttaagaaag cagatggata aagctatgga aggtcatttg gaggcatgta atgattaatt
     3121 ttgataaaaa agtagaccga ttgggtacat attgtaccca gtgggactat gtagaagata
     3181 gatttggtaa gaaggatttg cttcctttta caatttcaga tatggattta gagtttccat
     3241 ttgaaataac agaggtgttg caggaaaggt taaatcatag agttttggga tacagtagat
     3301 ggaaacatga tgattttaga aattcagtta cgaattggta tattaaaaga tttaattgta
     3361 gcgttgatag taactggata tattatagtc caagtgtaat gtattcaatt agcaaactta
     3421 ttgagatgtt aagtaatgaa ggagatggaa tccttataaa tactcctgca tacaatgctt
     3481 tttatgatgt gataataaat aacaatcgta atattataaa gtcacatctt gtaaatagga
     3541 atggattcta ttcaattgat tttaatgatt ttgagaaaaa gtgtaaaaaa tctaagatat
     3601 ttattttatg taatcctcac aatccaactg gtagagtatg gagtgagtta gaacttgtta
     3661 agatgatagg aatttgtaag aataatgatg tcaagataat atcagatgat atacacatgg
     3721 atattgtata tgacaataga atgacaccta tcttaaatgt tgcaaaggac tatttagatt
     3781 ctgtattcat ttgtacatca gcgtctaaat ctttcaatat accagcattg actgggtcat
     3841 atgtgattat accaaatgaa ggtattagaa ataaatttga aaatatcact agataccgtg
     3901 attttgtaaa ttcgccagca attctagcaa tattagccac aatggtctgt tatgatgact
     3961 gcgaatattg gttagatgaa ctattaaaat accttaaaga gaatcttaaa tatacgattg
     4021 attatataaa agataattta gaaccattga aattggtcat gccagaaggc tgttactttg
     4081 cttggataga tttttccgaa cttaaaataa gtggtgagaa attccaaaaa ttattgatgg
     4141 atattggaga agttgctatt atgtcggggt ctgtatatgg tgaagaaggt gagttttatt
     4201 taaggttaaa tgtggcatgt tcaagagaaa aacttgaaga tggtttaaat aggattaaaa
     4261 aaactatgga ttatattgaa aaaagaattt tatagtagga agggagatag ttatgggata
     4321 tttaaaaaca atggttgatg ttatgactac tgaaatgaat gccataagat gtttaataga
     4381 tgaagcagga atagaatatg aaagtatagt tagtgaaatt gcaaattgtg aaggcaaagt
     4441 aatttttatg ggtgtaggaa aatctgcaca tataggtaag aaattggcag ctacatttgc
     4501 tagtactggt acacctagct tttttgtaca tgcaacagag gcagtgcatg gagatttagg
     4561 aatgattgaa agtaaggata ttacaatttt aatatcaaat agtggaaatt ctatggaagt
     4621 ggtaaactgt ataaaatata ttaaagcaat aggttctaag acaattgcat ttacttcaaa
     4681 cagaaattca gtactggcaa aagagtgtga ttatgcttta atatatcctg caaaggatga
     4741 agcagaccat ttaaatctag caccaacaac atcatctaca attactttgg ttttaggaga
     4801 ttctattgca tgtgcattat ctaagagtag taattttggt tcttcagatt tttataagta
     4861 ccatccagga ggaagtttag gtgagaagtt aaaaacagct aacaatggat aaaagcaaaa
     4921 aggagagtag tttttatgtt caaaatgttt aaaaaaaaat caataaaatc acccatatca
     4981 ggaaaagtaa tagagttatc taaaatacaa gatggtgtat tttctcaaaa aattatggga
     5041 gagggtgtag ctatagattc tacaggagac attgtatatg ctccaatcaa tggaaagata
     5101 gtagtagtag cagagactaa acatgcattt gtaatagaat tagaaaatgg aatggaactt
     5161 cttattcatg ttggtttaga tactgtaaat ttaaaaggcg aaggttttga ggcattggtg
     5221 agtatgggag aacaagtgaa ggaaggaaca ccaatgttga aaattaaccg tcctttgatt
     5281 gaatcaaagg gtatatcact tattactcca gttacaataa ctaatcattc agagtacaat
     5341 atgaatacat gtaatgtagg caatagtgtt gaaggtggga aagatactgt aattgagttt
     5401 aaataaagaa ctaaaataca aggttaatat ttataaatac ctataaactt atgttttata
     5461 agattgattg tatagatttg ttgtataaaa aagaactctt taattataaa ttaaggagtt
     5521 cttttttatt ttgtaaatat tgtttagatt tatatagaaa aacacttagt aataaataga
     5581 ataaataagt atttatatag ctaaaaatct aaatagtttt gtttaaatat at
//
LOCUS       DAYAOX010000227         5610 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.228, whole genome shotgun sequence.
ACCESSION   DAYAOX010000227 DAYAOX010000000
VERSION     DAYAOX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.228"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(78..2297)
                     /locus_tag="ACGI57_003215"
     CDS             complement(78..2297)
                     /locus_tag="ACGI57_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9718268.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(2573..5227)
                     /gene="adhE"
                     /locus_tag="ACGI57_003216"
                     /gene_synonym="adhC"
     CDS             complement(2573..5227)
                     /gene="adhE"
                     /locus_tag="ACGI57_003216"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFG9718269.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT         1845 a          991 c          591 g         2183 t
ORIGIN      
        1 aaacactaat tatttagcaa tgatttatta aaatcaagca ttcataatag tgctttatat
       61 aaaaatcaaa ttttgtttta aagtaaatct aaaaaattct tagctggcat tggtcgtgca
      121 aaataaaatc cttgaactaa atcacatcca aattgtttca ttaactcagc ttgttcttta
      181 ctctcaacac cttctataat tacaactaaa tctagtgatt ttgctagaga cactattgat
      241 tttgctatat tgcgacttct ctcatcatgt tcaatgccac caagaaaaga cttatctagc
      301 ttcaatatat caattggcat atctcttaaa caactaatag aagaatatcc tgtaccaaag
      361 tcatccatag aaatagaaaa tccatacgtc ctcaactcat ttaaaacatt ctttaaaata
      421 tttaactcat taatagccac agtttctgtt atctctattt caatgaaatc tgaacttaaa
      481 ttatacattt ttattgtttt attgataaat tttacaatat ctggttgata caactcgacc
      541 ctagataaat ttactgaaac tggaactatc tttttacctt gctttttcca ttcatataaa
      601 tcattgcaaa ccctttcaaa tacaaatctc ccaattttta aaatcaactt acttttctct
      661 gctattggaa taaatactgc gggacttata aaaccatgtt ctggatgatt ccatcttatt
      721 agtgcttcac tccctatcat ttctccagtt acaagtgaaa acttaggttg ataatacact
      781 tcaaattgat tttttaccaa tgctattttt atatcatcta aaatcattga ttcctcacta
      841 agcttattcc taacatcttc attataaata gcataattta tatttttacc tttagcaaca
      901 gacctagcca tatttgcttt atttacagcc ttttgaatat ctacttcacc ttcttcaaca
      961 aaataaatac ccacacacag agaaatttca aagactatac tcaaattact ttcaatatta
     1021 cttcgtatat tatcaagttt tcttataatt ttatttcttc catttttata atcacagaaa
     1081 attgcaaaat aatcacttga aagtcgtgca tatacctcac cttctgttaa ttcttctttt
     1141 aaagcttttg ctattgtaat taataaattg tcaccaaatt catatccaaa agtatcatta
     1201 atcatcttaa agttattaat atcaaaatat actataatat aatttaaaga tggtttatct
     1261 aaaagatact tactacattc tctataaaat ttattttgat ttcctatata tgtaacttta
     1321 tcttcataag ccaaacttaa tatttctttt tcattagatt tttttatata aattatataa
     1381 aaaataattg caataaatat taatacaata gataacactg catatacagc tctgttgatt
     1441 atttgttttg aattactaaa tacaacagtt tctggaattg aaaaaattaa attccagtct
     1501 tttatataat tattttcact attcccatta tccatagtgg catatcctaa atatcttctt
     1561 tcttccaaca tatttaatac agttgcacct gttttatttt cttgcaattc tttttttaat
     1621 ctatttactt cttgaatatc attatcctgt tttaacaatt tataaatatt ttttgatagg
     1681 ctatcccctt gtggatgaag gataattgta ccattatttt ttacaacata agatatacta
     1741 ttattttcat aaaaagatat attaataagt ttcatcagag tattgatatt gtaagtacaa
     1801 tataaaactc caactacttt atcattattt aataatggta ctgaaatagc aatcttcttt
     1861 atagacttat ttacttcatc aacatatgga cttgaaacat acttcttacc tttaattgag
     1921 tttttgaaaa attctctatc tttcactgaa aacttatcac ctttggtatt ttcagcatta
     1981 ccatttatat caactattgc catagtgtaa aatttttgat tttccatttc atctaaaaga
     2041 tttttcattt tttcctcatt aggtatatct ggatttgaag cataatagtt agaaaaaata
     2101 tttaaaacat ctaaatcttt ttcaatttgt ctttgtgcta gttgagagcc ttgtactgca
     2161 acttctttta tatgtatttt tgcctctgac ataagaagat gttttgtatc ttctatatag
     2221 atatacccca ttgtcaaaaa taatgatgtt aaaaaaagaa ttgtaattaa tgtaaaactt
     2281 ttatttctcc ctgtcattcg actcctcccc taattagtca atacttcata tagataatat
     2341 atccttttta taccaattca tcatttatat tgtatataac tattcttaaa tgttacacat
     2401 aaaatcaaca taataaccat aaaaacattc ttttattatt ataataacac tatttaattg
     2461 aattttaaat taaagaataa aaactatcca ttttctcaaa caaaaaaact cttatacaat
     2521 aaaactttgt tttatgtata agagttttta ttttatctat tttatagcaa acttatcttc
     2581 cataatatga atttaaatac atatctttta tttcattcat aagtggatat cttggatttg
     2641 ctcctgtaca ttggtcatca aatgcttgaa ttaccatttc atctattgaa tccataaact
     2701 tagattcttc aacgccaaac tcttttatag atttaggaat accaacttta agttttaaat
     2761 cttcaatagc taccaataaa ctttggaatt tctcttcatc agaatttcct tttaatccta
     2821 aaaatgaagc tatttcagca tatctcttta atgcttctgg atatttatat tgagagaatg
     2881 ctcccatttt ctttggtgca tctgaagaat taaatttcat aacttctgtt atcaaaagag
     2941 catttgcaac accatgtggt acatggtgga aagcacctaa tttatgagcc atagagtgac
     3001 aaacacccaa aaatgcattt gcaaaagcca tacctgccat tgtagaagca tttgccattt
     3061 tttctctagc ttctgggtct tttgcaccat tatcatatgc acgtggaaga tattcaaata
     3121 tactcttaat agcttgtaaa gctaatccat ttgtataatc ggttgcaagc atagatacat
     3181 atgcttctaa tgcatgagtt aaagcatcta taccagaagc agatgttaag ctttttggca
     3241 tattcatcat catatcagaa tcaactatag ccatatttgg catcagttca taatctgcta
     3301 atggatattt tactcctgta tcttggtcag ttataacagc aaatggtgtt acttctgaac
     3361 cagtacctgc tgatgttggt atagctacaa aattagcttt ttcacccatt ttagggaatg
     3421 tatataccct ttttcttata tccataaatc tcatagctag gtcttggaaa tctacatctg
     3481 gatgctcata caatgtccac attatctttc ctgcatccat tgcacttcct ccacctatag
     3541 atattattac atctggatta aattctctca tagctttagc gcctatctta gcacattcca
     3601 aagttgggtc tggttctact tcaaagaatg tagtgtactt aatattcatt tcatctaata
     3661 aatcagtaac tgctttagta tatccattat tgtaaaggaa tgtatctgtt acaataaaag
     3721 ctctcttctt attcattaca tctttaagtt cttttaatgc tactcctaaa caaccttttt
     3781 tgaaataaac tttttctggt gctctaaacc aaagcatatt ttctctcctc tcagctactg
     3841 ttttaatatt aagtaaatgc ttaactccta cattttcaga tactgaattt ccaccccaag
     3901 acccacatcc taaagtaaga gaaggagcta gtttaaaatt atataagtcc cctataccac
     3961 cttgagaaga aggagtatta attaaaattc tacaagtttt catggctgaa gtaaacttat
     4021 caagtttttc tctttgattt acatcatcaa tatataatga agatgtatgc ccaaatccac
     4081 catcttctac taatttttct gcttttctaa ctgcatcatc aaaatcactt gccttgtaca
     4141 ttgcaagtac tggtgaaagt ttctcatgag caaaagcttc ttctatttca actgattcta
     4201 cttcacctat taaaacctta gtatctactg ggacttcaaa gcctgcaagt tttgctatag
     4261 tacaagctgt ttgcccaact attttagaat ttaatcctcc atttactaaa attatatttc
     4321 ttattttttc tgtttcatct ttatctaata aataagcacc acgctcttta aattcttttt
     4381 tgacttcatt gtatatattt tctaatacta taaccgattg ttcagaagca catatcatac
     4441 cattgtcaaa agtcttagat acaagaattg agtttacagc agtttttata tctgcactat
     4501 catcaattat tgctggagta ttacctgcac caactccaat agctggtttt ccagatgaat
     4561 atgctgattt aaccattcca ggacctcctg tagcaagggt caaatccact tctttcataa
     4621 cagttgtagt taattctaaa gatggctcat ctatccatcc tattatacct tcaggagctc
     4681 cagctaaaac agcagcctca agaacaactt ttgcagcctc tatagtagaa ttttttgctc
     4741 ttggatgtgg agagaaaatt attccatttc tagtttttaa agctaaaagt gctttaaaga
     4801 tagcagtaga agttggatta gttgtaggta caactgctgc aataacacct attggttcag
     4861 caattttagt cataccaaat gcttcgtctt tctctataac accacaagtt ttagtttctt
     4921 tatatgcatt atatatatac tctgaggcat aatgattctt tataacctta tcttcagcaa
     4981 ttcccatacc agtttctttt tgtgccatta tagctagagg aactctttgc ttattagctg
     5041 ccaaagatgc tgctaaaaaa attttatcta cctgctcttg actatatgta gcaaatattt
     5101 tttgagcttc tctgattttt gctagttttt taactaagct ttcaacgtta tttacagtat
     5161 ctaaaccaac tgacataact tcattttctt ttttattttc tattattact tgttctttat
     5221 gagccatttt aacaacctcc tgagatagtt ttttatttat tatgttaatt tattaacatt
     5281 atagcattta cttttcctat tttcaagtta tttttttaac attttatttt ttatctaata
     5341 taacatcatt tctactaata atatctagct ttacaaatac ttaatttaac tagatatcac
     5401 tttatttttt acgcaaatca ctaatgaaat catctaaaaa cttaaggata acgttatttt
     5461 tttaacgttt atatttttgt attttttgca aataaaactt tataaaaaga atttttaata
     5521 tgatactcta tcaagtttaa aaataaaaat ccttgaaaat gaaaattaaa acttaattaa
     5581 caagttaaag taaatatagt taaatgtaca
//
LOCUS       DAYAOX010000228         5541 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.229, whole genome shotgun sequence.
ACCESSION   DAYAOX010000228 DAYAOX010000000
VERSION     DAYAOX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.229"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..679)
                     /locus_tag="ACGI57_003217"
     CDS             complement(<1..679)
                     /locus_tag="ACGI57_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HFG9718270.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALS"
     gene            804..2186
                     /locus_tag="ACGI57_003218"
     CDS             804..2186
                     /locus_tag="ACGI57_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9718271.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            2588..3856
                     /gene="eam"
                     /locus_tag="ACGI57_003219"
     CDS             2588..3856
                     /gene="eam"
                     /locus_tag="ACGI57_003219"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016869 - intramolecular aminotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006536 - glutamate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HFG9718272.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDTCDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(3949..4305)
                     /locus_tag="ACGI57_003220"
     CDS             complement(3949..4305)
                     /locus_tag="ACGI57_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718273.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(4456..>5541)
                     /locus_tag="ACGI57_003221"
     CDS             complement(4456..>5541)
                     /locus_tag="ACGI57_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFG9718274.1"
                     /translation="KKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPFIIA
                     FVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADLVAQ
                     IPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFSTTFS
                     VGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLNIGK
                     YFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVIINL
                     FSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIPGMI
                     LSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         2177 a          748 c          797 g         1819 t
ORIGIN      
        1 aacttagtgc ggcttctatt gcatgtatta ctgtcgtatc aaatagtgga atagaagtgt
       61 cattttgttt aattaaaaga cctatttctg tacatcctaa tataacagcc tctgcacctt
      121 gtttgccaag cttatctata atagataaga aagctttttt tgaagattca gatataattc
      181 ctaagcaaag ttcattaaat attgtgtcat ttactattat acggtcttct tcatttggaa
      241 ttaagacttc aattccatta ttaataatta catttttata aaaatcttgt tccattgtat
      301 atttagtacc taataaggcc acttttttta tttctttttc ccttaatacc gtagcagtta
      361 catctgctat gtgaagtaat ggaatatgta tactttcttg tattttatct gataccttat
      421 gcatagtatt agtacagata acaataaaat ctgcccctgc ctcttgtaat ttaattgcag
      481 catcagctaa aattttggcg cttttttccc aatttccact agattggcat tcttcaattt
      541 cttgaaaatc tacactgtaa agtatacatt tagaagagtg tagtccacct aatctttctt
      601 ttattactgt attaataact tgataatatg taatggtact ttcccaactc ataccaccaa
      661 ttaagcctat agtcttcata ttatacctct cttatttatt tattttgaaa tagctattta
      721 caaatacaag tatagcatct aaaatagaag catacaatga taaaattgta tggagtacaa
      781 ttataattaa gaggagtaat tatatgccaa taaattcttt tgaaaactat cctatgacat
      841 ggaaaccaaa attagataac cgaaaaccac caatatataa aactttagca atgttattag
      901 aagaagatat taaaagagga aatttaaatc ctggagataa actgcctcca caacgggaat
      961 tagcagattt tctagattta aacttaagta caataacacg agcatttaaa ctttgcgaag
     1021 aaaagggact aatatgtgca aaagtgggaa agggaacatt catttcttct gatgtaaatg
     1081 taagtaatac attattatat caaacggaat caaaagacat aattgaatta gggacagttc
     1141 atcctccgta tgagcaaaat acatatatta ttgactttat taaaaatgta ctaaaacaac
     1201 cagaaatgga taggttttta caatacatgt ctccatctgg cacatatatg caaaaaaagt
     1261 caattgcaaa atggatagaa agaaataatg tatacaccag tgaagaaaat atattattat
     1321 ctacaggagg gcaaaatgct atatgtgcaa ctttactagg attatttaaa gctggtgata
     1381 gaattgctac agattcttta agtttttctg gaattaagtc aatagctaaa atgataggta
     1441 ttcaattagt cccaattcct caagaaaata atgaaatatc gattgaatat cttgaaagtt
     1501 attgtaaaaa tgaaaatata aaaggcatat atcttatacc agattatcat aatccaacga
     1561 cacatactat gtctgattta tctcgtaaaa agatagccaa gatagcaaag caatataata
     1621 taataattat tgaagatgct ataaatagca tttttagaga tggtatacaa actcccatat
     1681 tttctttagc tagtgataat actatatata tttttagtac atctaaattt ttatgtgctg
     1741 gtcttagagt agcatttgta gttgctccaa aacgatatat agaaaattta gaaaatgcac
     1801 tatataatat gaatctaatg gtttctccat ttacagcaga aatagtacac cgtttattat
     1861 attctcctat tatagataaa attatagaag agaaaagaga tgcaattata gaaagaaatg
     1921 aagttgctga taaagttttg tcagattata atttgattgg tgataagaat tgcagttttc
     1981 gttggttact tcttcctgat gaattggatg gaaaatcatt tgaaatttct gcaaaaaatc
     2041 taggagttca agtatattgt gctgaacgat tttcagtagg aaactctaca gttccaaaag
     2101 cagttcgtat ttgtgtaaca gctccaaaag atgtagaaga attagaaaaa gggcttaata
     2161 taataaagtc acttcttaag agttaaattt attttactat tatttaatta aataaataaa
     2221 aaggcggaga aatttattat aataaacctc cgcttttatt atttaatgaa atactttact
     2281 ttttattaaa aaatttaatt ttaataatct tgaaaatata atcgttttat ggtaaaataa
     2341 ttcttaaaaa tagaaaaaat taatcatttg ttaaaaaaaa tgcataattt attgtaattt
     2401 attgacgaag gttgaaaaat attcataaat gtgcgaaata acagaaaaaa tgcaaaaatt
     2461 aattcaattt catttgttat aaaatctata tggcaaaatt aaatctgcct atcagaatga
     2521 tataaacaca ccagaaagga ggacgctatc aaataataca agtagttaac tttgatagca
     2581 aaaaattatg aatgaacaaa ctagaatatc cttagagaga gctgctgaat taaaatcaaa
     2641 aattgatgat tatattcagg ctagaaaaac gattaacaga ggtcttgaaa aagaagaaga
     2701 gataaataaa cgaaaacaga aaatattaag tatcttaaat ggaactgaag aggattggaa
     2761 taactacaaa tggcaattat caaatagaat aacagatgta gatactttat caaaaattat
     2821 aactctaact aaaaaagaaa aagaatatat aaaagaggtt ggtactcaat ttagatgggc
     2881 aatatctcca tattatttga gtcttataga cccagaagat acatgtgacc caataaaatt
     2941 actgtctata ccaacacata tagagttgga agatgaacaa gaagatttgg acccaatggg
     3001 agaagagtat acaaacccag caggatgtat aactagaaga tacccggata gattaataat
     3061 aaatgtaaca aatgagtgtg ctatgtattg tagacactgt cagagaagaa gaaatattgg
     3121 acaacaagat tctcataagt caaaagctat tatccaagaa tctatagact atatcagaga
     3181 aaatgaagaa ataagagatg tactagtaac tggtggagat gctcttacat taaaagatga
     3241 ttatttagag tggattctta gccaacttaa agagatacca catgttgatt atgttagatt
     3301 aggtactagg actcttgtta caatgccaca aagaatcaca gatgaatttt gcaatatgct
     3361 aaaaaaatat caccctgtat atataaatac tcattttaat catccaatgg aaataactaa
     3421 ggaatctaaa gaagcttgtg aaaagttagc aaatgcagga gttccattag gaaatcaggc
     3481 agtattatta aatggaataa ataatgataa atttgtaatg agatgtttaa atcaagaatt
     3541 actgaaaata agagtaaaac cttattatat attccaaagt aaacatgtaa agggaacaaa
     3601 acatttcaat acatcagtag atgatggtct tgaaatcatg gagtatttaa gaggatatac
     3661 atcaggaatg gctataccaa catatatagt aaatgctcca aaaggaggcg gaaagactcc
     3721 tttgcttcct caataccttg tatcaaaagg aacagattac gttatgctta gaacatggga
     3781 aggaaaagtt ataaaaatgg aagatgaacc tgctgtagat ataaagaaac ttataaaaga
     3841 acaagcgcag gattaaaaaa gtatttaaag tttataaaaa gaaacaactt taaaataaaa
     3901 agaaaatgga tgtcaaattt atatcaattt gcatccattt tttattagtt atttattatt
     3961 ttgttgttga ttaactaatt caagatatcc acttgggtgg aagtaatata tctcagaagt
     4021 attttcattt acaaagtaac caatatcacc atctgcatct ggtaaaaata cataaccatt
     4081 aagattttta gcttttaatg cttcaaaatc actagcattt ccttgatata tgtaatcaac
     4141 ttcattgttg tattttacta aaagttcttt agcttgttct tgagtcaatt tagtattttg
     4201 gcttacagga acattctttt gagaatctgc ataagaagat tcatttactg gtgcagttaa
     4261 taacaaacaa gataaaacta ataaagatat aagtgctttt ttcatactaa aaccctcttt
     4321 catgtttaat tatataagat tcattactta ataatattgt atgtttccta taattaaata
     4381 ttaccattaa agagaataat attacagtta cacagctgta acttttgatt acaaaatcgt
     4441 aattaaattg tatttttatt ccatttgtat tttatcatgt ctacgattta caaactcaca
     4501 gaagtatatc tttataacgc ccatcacagg agcagataga atcataccag gtataccaaa
     4561 gaatcctcca ccaacagtaa ctgctaggat tgtaaagaat gggctaagac ctagttgtcc
     4621 acctacgatt tttggctcta taaatgtaac ttcaacttgt tgaattatta ttaagtaaat
     4681 taaagaagtt aaagcaattg ttggattgga gaataaatta attattacaa ctggtgccat
     4741 acctattact ggaccaaaat atggaaccat attcatgaca cccataagtg taccaaataa
     4801 tagtgcatat tcagatttta aaaagtaaag accaattcca gaaagtactc caactataaa
     4861 agaatctaaa atcttacctg taaagtattt accaatattt aaatttaagc tttgacatag
     4921 ttctattata aagtctccat attttttacc taaggaaata taaactactt tttttgtaaa
     4981 cagtaagaat ttttcttttt ctaataatat atagaaacaa attataaaac ctaaaagaaa
     5041 ttgtactata aattttccaa ctgaaaatgt agtgctaaat atcccaccaa gtgaaccaat
     5101 aagcaagtta ctaaatttag gcattacact cataattttg tcaccatact cttttaatgt
     5161 attagggtca actgattgta aactcttgcc taagtcgaat aaaaactgct ctgttttatc
     5221 tatgtatgta ggaatttgag caactaaatc agctaaacta tttactatgg atggaactgt
     5281 aaataatata aacgaaatta gtataactat gagtacacca taagtaagaa gtaacgaata
     5341 tattctctta aaatttaatt tactttctaa gaactttact agtgggttga atatatatgc
     5401 taatacaaat gctattataa atggtgttag tagtgacatt aataaactta tcactccaaa
     5461 aaaatatttg taattatcaa taactttaat caagatatat gaaacaacta cagtgagtac
     5521 aatatttaga taaatttttt t
//
LOCUS       DAYAOX010000229         5533 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.230, whole genome shotgun sequence.
ACCESSION   DAYAOX010000229 DAYAOX010000000
VERSION     DAYAOX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.230"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            19..243
                     /locus_tag="ACGI57_003222"
     CDS             19..243
                     /locus_tag="ACGI57_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718275.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            296..820
                     /locus_tag="ACGI57_003223"
     CDS             296..820
                     /locus_tag="ACGI57_003223"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9718276.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(925..1071)
                     /locus_tag="ACGI57_003224"
     CDS             complement(925..1071)
                     /locus_tag="ACGI57_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718277.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(1210..2136)
                     /locus_tag="ACGI57_003225"
     CDS             complement(1210..2136)
                     /locus_tag="ACGI57_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFG9718278.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            2326..4197
                     /locus_tag="ACGI57_003226"
     CDS             2326..4197
                     /locus_tag="ACGI57_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFG9718279.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(4289..5224)
                     /locus_tag="ACGI57_003227"
     CDS             complement(4289..5224)
                     /locus_tag="ACGI57_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFG9718280.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
BASE COUNT         2215 a          592 c          890 g         1836 t
ORIGIN      
        1 taaggagtag ataaaaatat gataataaaa cttactaaag acaattttaa ttttagattt
       61 aaacaatatg aaggtatgtt ttttcatgct gaagtagttg aaggtatgga atccattaga
      121 tataaaataa ctaaggataa agaaggtaca gaattaaaag aatttggaag tacacatata
      181 aaagataata tgatatatgt acctcaggta gaagcataca tagatttgaa cagtataaaa
      241 taaagatgta aaattttaat taaaaataaa gattatgttt gagggggaat ccaatatgag
      301 taagcctata gatataacaa taagacctat aagaagagaa gatgcacaag cttttaatga
      361 aattagaaga acatacagtg ttatgagaaa tactttggca ttaatgagtg acagagtcga
      421 taaaactgaa gctatgttag cttctttggg agaaaatgat tatatgtttg tagctgaaac
      481 taataataat ggagaaaaag atgtaatagg ttttgctggg ttacatgtaa attcttctcc
      541 acgattgaga catagcgcag aaataggaat aagtgttgca gagaattatc agggaagagg
      601 agtaggtaag aagcttatgg aacaactttt agatatagca gataattgga ttatgttaac
      661 tagagtagga ttagaagtaa ttgtggataa tgaaaaggga ttaaatttat ataaaaagct
      721 aggttttgaa atagagggaa ctaaaaaata tgctgtaatc agagatggaa aatttgaaga
      781 tgtttatatt atgggaagat ataataaaaa tttaatttaa agaagctaat agagaagtaa
      841 aatagagtgt ctttaataaa aaatttacat taaagataat ctattttact tctctattgt
      901 gttaaaaaac ttatttttaa atgtttacaa aaacattcta tatacaaata aaattattag
      961 caatatcaac gctattttaa aagctaaact agcaacagtt ttaatgattt ttacagaaac
     1021 acctatagca actatagcaa atactatttt aaagagtaaa tccatatcca tatttaagcc
     1081 tcctattcta taatacatat agatattatt gacaaatatc taggaaaaaa ttccatcaat
     1141 atactacaat aaaatgttta tatttgtaat ttattgaata tgttattata taatacataa
     1201 aaaaataaat caagataaga gtaaaatact tataaaaggt atagatataa ctgaaaatag
     1261 tgttgtaaaa aacacacatt gtgaagctag tgttatattg gagtcatatt gattagccat
     1321 tatagcagta tttgcagcag caggcattga agaaattact actggtatag ctaaaatcat
     1381 cttatcattt atccatcctt ttagaataaa atacacaatc acaggaagta ccaataatct
     1441 tataaaagtg actatatata attttttgtt gacaaaacaa tcgagagctg aagaattagc
     1501 aagtaagctt ccaatgataa tcatggatat tggtgtagtt atacttccca acatctcaag
     1561 tggctcgttt ataaattggg gtaacctaag tcctgttaca aataaaaaaa gtccaataac
     1621 aatagctatt gtagcagggc ttataagtga ttttattgaa aaacctctac ttgtactgcc
     1681 tttagataaa agataaattc ctaaagtgaa agaaaataag ttaaaaggca aattgaatat
     1741 tgcagtatag aatattgctt catgacctaa aactgcttca attacaggat aacccataaa
     1801 agcaacattt gaaaaaacaa ctatatactg gtatattccc aaatcttttt tatcattaca
     1861 ttttaaaatg tatttcaata aaaatgcaat tataattgaa actatataca taatcaaaga
     1921 tgtaaataat aacaatagta ttttttgtat cattttacta tcaaagttaa tttgcatgga
     1981 gctaattatc attgatggta aaaaaactgt cattgtcaaa gttgaaagct tagatgtgca
     2041 gtggtcatct aataagttga attttctgac aaaatatcca actaaaataa ttataaataa
     2101 aactgcaact tggataaaaa tattgctgaa attcataaaa tgtcccccct atatatacat
     2161 tatataccaa ttacataata aaatctaatg attataaaat ttaaaattta atgttacaat
     2221 atagtgacaa aaggtaaaaa ttcaatatta tagcattaat tgtagattat aatgtataat
     2281 aatgtttgag agaatatatt ttataaaatg ggtaggtagc gaataatgaa aaatacaaaa
     2341 ttttctataa aaaaaataaa aaatatatct aatgacccga aagtcgtaca tactaagggt
     2401 gccctccttg aaaagatggg taagatggaa agcgcaatag agttgtataa gagtgctgca
     2461 ttggataatt acgtaaaatc tcaatatact ttaggaaata tatatgaaag taaaaaacaa
     2521 tttaaagaag cagaaaagtg gtattcgatg gcatataaaa gtggaagtga agatgctgct
     2581 tttgatttag gtaatatgta ctataagtta gatggctatg aatatgctat atattggtat
     2641 gaaaagatag caaatttagg ttatttacaa gcacaaaata atttaggtgt atgccatttt
     2701 aaaatgaaag attttgtaag gtgtgaaaaa tggttaaaaa cagcagcaga caagaattta
     2761 ggtaaagctt gttttaattt aggggtatta tatacttttt tggagaaaga tgatgaagca
     2821 tatgaatact ataaaaaagg ttctgtgctt ttagatgatg atgcaaaata taatttagca
     2881 atattaaatg gaaaaaagaa agataataaa tcaacagtaa gtttatataa acagttgtac
     2941 aagtcaggtc atatgaaagg ttgctttaac ttgggtatga ttatggaaat aaatgataat
     3001 ttagaagatg cagagaagta ttataaaaag agtgctgata gagatgatgt gaagtctgag
     3061 tataggcttg catatgtata tgatagaaaa gaagagctgg gagatgcaat ttattattat
     3121 gagaaagcaa tagaaaaaaa tcatacactt tctaagtata gacttgctaa tttatataat
     3181 agagaaaatg agatagagaa ggcaaaaact tattataaaa tggctgctga agatgatata
     3241 acagaagcaa aaaataatct ggcaggtata tattttgaag aaaaagatta tgaaaatgct
     3301 attaaatatt atgaggatgc tatagctgtt ggatgcaaaa gttcccttga aaatctaggg
     3361 gatttatatt atcagaatca agatatagaa aaagcgattt cgtattattc gaggattcca
     3421 aataatgtat cttgtcaaat caaattagga aatatctatg aagatttaaa taatattgaa
     3481 gaagcaataa gttggtataa gaaagcatct gaaaatggag atacgaggtc tagttataga
     3541 ttggggtgta tatatgagag cttaggaaat acaaaaaatg ctagaaagta ttttgaaatg
     3601 gcaagtagca aaaaccatat gaatgctcgt atacatctag gtagaattta ttttagagaa
     3661 ggtaaattgg aagagtctaa aatgatgttt gatacacctg ccaatgaaaa taatgtgtat
     3721 gctcaacata tggtaggatt aatttatgat atgttctata aagattatgt aaattctaaa
     3781 ttttggtatg aaaaggctag agcacaaggt tgtgttgaat caatatataa tttaggtcaa
     3841 atttatttaa aattaaatga tgacgcagaa gctgagaaat attataaaga aggtattaaa
     3901 tatggaagta agaagtgtga gtatatgctt gcaggtcttt attataaaaa gagcctagat
     3961 atgtacattt ctttagcaaa tgaagagtac gataattgtg aagagatagt agaaggcttt
     4021 ccaaatttaa atatagactt tgatgaagta ttaattcctc catttaagtt gcaagaagag
     4081 gtaatagatg aagaagagta tgtaccaatt tatatactaa atataaaaga aagtttagat
     4141 tcaatgctag aagggcttga aactgaaatg attatagatg aagaacatga tagttaaaaa
     4201 gtattataag attatataga tttaaatata taaaagccat taggataaat gtaaatcaat
     4261 taattctaat ggccttttta ttaatatttt ataatagctt atttcttgac atgaaaaatg
     4321 taatcactat acatattata gaggtaataa gaagtattat ccaaaatccg tttgggttat
     4381 tagaaaatgg catgttgatg acattcatac caaagaatcc tgatattatt gtaggtattg
     4441 agaaaattag tgtaactact gttaaaattt gcataacatt attctgatta ttacttataa
     4501 ttgcactgaa agcatccata attctactca atatatctcc atatatttta gccatatcaa
     4561 gagcctgtct attttcaact ataacatctt ctaataggtc ttcatcatct ggatacttct
     4621 ttataccagc agttctaacc attttgttta aaacaagttc attagatttt agtgaggtag
     4681 agaagtatac caaagatttt tctaattcca ataattgaac caattctttg tttttcattg
     4741 atttgtaaat ttctcgctct attatgattg tcattttatt tatttttctt aagtaatgca
     4801 agtaataagc tgcatttttg tgaagaattt gaagcaaaaa gcgagtcttt ttaaacgtaa
     4861 aaaaatcttt aatatgacca actataaagt catttaaaat cttagtttgt gcatcacaaa
     4921 ctgttacaat agcttcttca gttagtataa tacctaaggg tattgtagta taatgagaag
     4981 aattagagtc actttcgtct actggtatat ctataagtat aagagtatga ttgtcttcaa
     5041 catctattct tgagcgttct tcttcatcta gtgcagattc tatgctttct acatctatat
     5101 ttaataaatt agaaatttcg ttgatttcac tatgattagg ttcaacaaga tttatccaac
     5161 agccatcttc aaaaaaacta agtttctcta attttgaatc tatagttttg taatatctta
     5221 tcatggcaac acatccttta ttcagtttta tataaatctc atcatttaat agtatctatt
     5281 aattaactaa tttatataac tgaatatatg tattagtttt gattttagat attattaagt
     5341 gatgaaaatg taaaaaattc tctccgcaac ggactagtat ccattgccca tcactctcct
     5401 tatttattat tttatatata gctatttatg aaatgcgtat tttaagcact ctttaatata
     5461 ataccttttc caggtatata aatcaagttt tgatttgatt aaataatatt tttaccataa
     5521 tttacacata aag
//
LOCUS       DAYAOX010000230         5462 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.231, whole genome shotgun sequence.
ACCESSION   DAYAOX010000230 DAYAOX010000000
VERSION     DAYAOX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.231"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..1018)
                     /locus_tag="ACGI57_003228"
     CDS             complement(<1..1018)
                     /locus_tag="ACGI57_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFG9718281.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKL"
     gene            1085..1456
                     /locus_tag="ACGI57_003229"
     CDS             1085..1456
                     /locus_tag="ACGI57_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFG9718282.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            1493..2167
                     /locus_tag="ACGI57_003230"
     CDS             1493..2167
                     /locus_tag="ACGI57_003230"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFG9718283.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            2416..3255
                     /locus_tag="ACGI57_003231"
     CDS             2416..3255
                     /locus_tag="ACGI57_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HFG9718284.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            3499..3595
                     /locus_tag="ACGI57_003232"
     tRNA            3499..3595
                     /locus_tag="ACGI57_003232"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3533..3535,aa:Sec,seq:tca)
     gene            3745..4452
                     /locus_tag="ACGI57_003233"
     CDS             3745..4452
                     /locus_tag="ACGI57_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFG9718285.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(4594..5430)
                     /locus_tag="ACGI57_003234"
     CDS             complement(4594..5430)
                     /locus_tag="ACGI57_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9718286.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
BASE COUNT         2079 a          645 c          845 g         1893 t
ORIGIN      
        1 taagtttaaa attatctttt atacccttag ttaagtatac tttcttatcc ttagttatga
       61 atatagcatc tacattatca attttattaa ttaaattcat acctttttca agaccaagac
      121 caaatgtaga agtggataag gcatcacaat taatagattt atcagagatt attgtaacac
      181 tacttaaatt gttttcaaaa ggataccctg taaatggatt taacatatga tgatatattt
      241 ttccgttttt ttcgataaat ctttcataaa ttccagaagt aacaacagac ttatttgtag
      301 tttctatgtt tcctatggat tcaccatttt tactagttgg gtcttgaata cctattttaa
      361 aaggtttatc atttttacca ttttctaaag taaatatatt tcctcctaag tttactaaac
      421 ctttttttac gtcctctgat tttaaatagg ctacaatttt atcagcagca tatcctttag
      481 ctatggcacc taaatcaatt ttcatatttt ctttggttaa ttttatagat ttattttttt
      541 catccaatat gatgttctta taatcaataa gaggcagaag tttttgtatc tcagaatcac
      601 ttggaacttt agctttatct gttccaatag cccataaatt tgataatgga ccaattgtta
      661 tgtcaaaata tccatctgat aatttggaat attgtatagc ttcttttacg acgaaaaatg
      721 tatcatcaga aactctgaca aattttttac cagcattatc atttatttta gaaacatcac
      781 ttcctggtat atgagtgctc attttgttgt caatatatct taaaattgaa tcacattcat
      841 ttaagatttt atcacttttg gattctttta cattaaatac agtaacttcg tttactgtac
      901 caaggtagta gctagtttta ctatatttag tattctggct tgaactccta aaaaatgaaa
      961 ataaaattaa tgctaaaatc aatatcacaa aaataaatgt gatttttttc ttattcatgg
     1021 tatattcctc ctatgaaatt gtgttattat tttattataa cataatttca taggaggaca
     1081 aaaaatgaag aaaaaagaca ttcttcttat tttaggcatt ttggttgtta ttacagcatg
     1141 ttatgggata ataaatgtta taaattctaa aaatgctgga aatattgaga tttatgtaga
     1201 taataaattg tataaaactg tttcaataaa tgccaaagaa gaatttaaaa tagagaatag
     1261 aggggggtat aacatagtaa agattcatga taaaggggtt gagatagtag atgcttcatg
     1321 tcctgataag gtttgcgtac atactggatt tataaacaag ccaagtcaaa gcatagtttg
     1381 tatacctaat agagtgagca taaaaataaa tacaaatgaa aagaatgata atcaagaaga
     1441 tataatttct aaataaaata ataaaaataa ttgaaaggaa ttggtgataa ttatggttaa
     1501 cttaggaaac gattgggatg aattattgaa agaagaattt gaaaaagatt actacttaaa
     1561 cttaagaaaa tttcttatag aagaatataa aacacgccaa attttcccaa atatgcacaa
     1621 tatatatgaa gctttaaaac acacttcata caaagacaca aaagtcttaa tattaggaca
     1681 agacccttat catggagata atcaagctca tggtttagct ttttcagttc aaccacaagt
     1741 gaaaactcca ccatctttat taaatatgta taaagaatta aaagatgatt taggttgttt
     1801 tatacctaat aatggatatt taatgccttg ggcagaccaa ggagtgttac ttttaaatac
     1861 tgctcttact gttagagcgc atgaagctaa ttctcataag aacaaaggat gggaaatttt
     1921 cactgataga gtaatatcta ttttaagtga gcgtgaagac ccagttattt ttgtactatg
     1981 gggaagtaat gcacgtaaaa aagtagagtt gattgataca agtaaacatt atattttgga
     2041 agcacctcat ccaagcccat tgtcagcaag taaaggcttt tttggatgta aacacttctc
     2101 taagataaat gaaattttga aaaagctagg aaaagaacct attaattggc agatagaaaa
     2161 tatataaaag taaaaggtaa caaaatgata acaaaacaac aataaacaat gacattgttg
     2221 taactaatag aaatatgctt aaatagttga aaatactatg tatgcatatt tctatttttt
     2281 tgtcaagttg acaactcttg aaaaaatagt tacaatttag ttacacaaaa aacacaaata
     2341 aaaaaagaaa cttacaaagc agaattttat gaagcaaaat atatagtgta aaaaatctta
     2401 gggaggaaat tttatatgaa ttttaaaaaa ttgagtaatg ctaaagtctc ggtaatggca
     2461 gttgttttat cagttggaat attatcggga tactctgcgc ttaataaaca agtaacatta
     2521 gtagtaaggg gagaagaaag aactatatca acatttagtt ctaatgtagg agacctttta
     2581 gaatctcaaa acattagcta tgatgcaaat gatatagtaa ctttagatga aaatacaaag
     2641 ttatctaatg gagataaaat agaagtaatt gatgtaaagg aaaaaacagt tgtagaaact
     2701 aaagacattc catttgaagt tacagttgtt gaagatggct ctttattaaa gggtgattct
     2761 aaggttaaag aagaaggaaa gtctggtaaa agtgaattaa tatataaaga aacttatcac
     2821 aatggaaaga aagtagaaaa ggaatttgtg aaaaaggttg ttaaagctga gccagtaaat
     2881 aaggttgtaa gcaaagggac aaaggtagaa atgcaagtag cctcatcaag gggagaaagt
     2941 tcaagaagaa tggcttcatc atcatctagt agcagtagta gtaacaatgg taaaaatatg
     3001 agagtggtag caactgcata tactggtcac agtataactg caacaggaac aaaacctaag
     3061 tggggaacaa tagctgtaga ccctaaggtt ataccatatg gaactaaagt ttatatacct
     3121 caatttgaca tggtgtttac tgcagaagac tgtggtggag caataaaagg aaataaaatt
     3181 gatatctaca tgaatgattc aaaaacagtt tataactggg gaagaaaaac tatagatata
     3241 cgtatattaa aatagttaaa taatttatat ttttactgga aattatattt tgattatata
     3301 gagaagaaac tctttataga ttctaaaaat ataatttcca gttttttatt ttggatatgt
     3361 aataaaacag gtaaatttta accatgtata gataaaagct atttaaatta taatttacta
     3421 tcatataaat ctataaaaat tgttgcaaag agtcaaaata tatgatatta tagatttcgt
     3481 tgagaaggat ttcattatgg gagtggatgg gtgccggtgt gccctctagt cttcaaaact
     3541 agtatgaggg gttaagagcc tcttaggtgg gttcgattcc cacgcattcc cgccaatatg
     3601 taaaaggtat cttttaatta aaagatacct ttttaaattt gttttaagat aatttagttt
     3661 taatgatata taatattttg tattatataa tgtaaatttt ataatactat tattgttcag
     3721 ataaaagtct aggagagtga aaagatggaa aataatttta caacgagaac tagttttcta
     3781 gttggagatg atgggataga aaaacttaat aattcaaata taatagtatt tggagttgga
     3841 ggagttggta gttttactgt tgaagcttta gcaagagctg gagtaggcaa tattactatt
     3901 gtagattttg atgatgtaga tataaccaat ataaatagac aaataccagc cctacattca
     3961 actgtaggaa gatacaaagt tgatgtcatg gaagaaagaa tattagatat aaatcctaat
     4021 atcaatataa aaaaaataag aagtctttat aataaagata ctagtgacga aatactaact
     4081 gaaagatatg attatgttgt ggatgcgata gacatggtaa gttccaaaat acatctaatt
     4141 gaaacttgtg aaaaaaaagg attgaaaata ataagttcta tgggtatggg aaataaatta
     4201 gacccaacta aaatagtcgt tacagatata cacaaaacaa gtacttgtcc tctagctaaa
     4261 gttatgagga aagaacttag ggatagaggt atcaaaaagt taaaagtagt atattctact
     4321 gaacaaccaa tagaacttaa gaagaaagta atgaatggta gaaaagtaac acctggtagt
     4381 gtatcatttg tcccatctgt aggaggttta ataatcgcat ctgtaatagt aaatgaacta
     4441 ttaggacaat aagttttaaa tattttaatt tataaatttt atatacaaat ttgaaggcta
     4501 gaaggatgaa tataaagcaa ttttatattc atacttttag cctagttatt ttatattacc
     4561 ttattttatg tagttgtagc tttatttttt agattatgta tctatttcat aattatattt
     4621 tgtaaatata atttctccat cactataaaa aacaatatct cctattctgt ctgttctata
     4681 aattgataca tggttttgtt ttagattatt aataacctcc ctatgtggat gaccatattg
     4741 atttttatat ccacatgata ttgcagctat atctggacta atttttttta taaattctaa
     4801 agatgaagaa gaattacttc catgatgacc tatttttata aaattaatat tatttaattt
     4861 aaaagaatgt aaaatttctt tttcattttc ttcttcagca tcacccataa ataaaaaaga
     4921 attatcatta aaagttaatt tgaaaactat agaatttaaa ttagattctt cttgtatata
     4981 tgatggactt aatacatata tatttatatt gttatctatg tttaaaacat catttttata
     5041 gagatgctga atagataagt ttttatccgt acaagaattt attagatttt gataagcctc
     5101 actatcagtt gattgttcag gcatatatat ggagtttaca ttaaattttt ttataatatt
     5161 atcaagactt cctatgtggt ctgaatctgg atgagttgct attataatat caatagtttt
     5221 tattcttttt tgtctaagat aactaataat tatattttcg ctatcttcat cacctccatc
     5281 aattaatata ttcttatttg tgggtgtttg aactaaaata ctatcacctt gtccaacatc
     5341 aatcatatgt atagatagta gagatttttt gtcacagccg attaaaagtg atattataat
     5401 aagtattgat aaaaaaattt tatttctcaa taaaaaatca ctccattcat tacatgttta
     5461 ta
//
LOCUS       DAYAOX010000231         5424 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.232, whole genome shotgun sequence.
ACCESSION   DAYAOX010000231 DAYAOX010000000
VERSION     DAYAOX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.232"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            95..1276
                     /locus_tag="ACGI57_003235"
     CDS             95..1276
                     /locus_tag="ACGI57_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9718287.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1418..2335)
                     /locus_tag="ACGI57_003236"
     CDS             complement(1418..2335)
                     /locus_tag="ACGI57_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotA family spore coat protein"
                     /protein_id="HFG9718288.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2588..3382
                     /locus_tag="ACGI57_003237"
     CDS             2588..3382
                     /locus_tag="ACGI57_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718289.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3383..>5424
                     /locus_tag="ACGI57_003238"
     CDS             3383..>5424
                     /locus_tag="ACGI57_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HFG9718290.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLD"
BASE COUNT         2068 a          622 c          903 g         1831 t
ORIGIN      
        1 gaaatagaat gcttatagtg gtaaaattaa ttattagaat attagaaata ataaataaat
       61 aatacattgt acttaaaagg gggagaaatt taagatgaac aaaaaatata ttaaagcagg
      121 gaatttaatt gatggaaccg gaaatagtat tatgcaaaat aagggtataa ttattgatgg
      181 agatactata gttgaaattg aagatataag agatgattta aatggatatg atgtgtacga
      241 ttacttagat aaaactgtta tgccaggcat tatgaattgt catgttcact tgacaatgga
      301 gccagttgga aatccaaaga tatattatga taatgtatca gatgtagagc ttgtagtaaa
      361 tacgataaaa caacttgatg cttatttgga ttcaggagta acatatatta gaagcttagg
      421 ttgtcccaaa tatgtggatg tacaacttaa aaatcttata gacaaagggt ctatagatgg
      481 tcctggtata gttacttcag gtccagttat atgtatgaca ggagggcatg gatattattt
      541 tggtattgaa agtgatggag tagatgaatg tagaaaatca gccagaacag ttttaaaaaa
      601 tggagtagat tgtgtaaaga taatggcaac tggaggtgta acaactgatg gagttgagcc
      661 agggtcacca caattaactt tggaggaaat gaaggctgct gtagatgaag ctataaaagc
      721 aggtaaaata actgccacac atgcacaagg aagaactggt ataaaaaatg cagtgcttgc
      781 aggaattact tctatagaac atggtgttta tttagatgat gaaataattg atttgatgct
      841 tgaaagagga acttacttag ttccaactgt ggctgcacca tattttattc taaatgcagg
      901 aaaagaaagt ggaataactg aagctactct tagaaagtgt gagattgttg ccattccaca
      961 caaagagtca ttcctaaaag catataaaaa aggaattaaa attgcagttg gttctgatgc
     1021 aggaacttct tataatgaac atggaaaaac atattatgaa atgaaactaa tgtcagatta
     1081 tggtatggat aatatggata taatagttgc tgctacaaaa acagcatctg aacttttaag
     1141 aattgataaa aattatggaa ctctagaaaa aggaaagaaa gcagatgtta tagttgtaaa
     1201 tggaaatcca gtagagaata tagacgtact tgcagatgtg ttagcagtat ttaaacttgg
     1261 taaaaaagtt agataaatag tataaaaata aaaaagagtg tctaaagaaa tttttaaata
     1321 attatagaca ctctttttta ttagtaataa ttaaatcttt tatactttag aaaatagatt
     1381 ctattttata tttagatata actctttaaa atcattctta tgcaatataa tctatagaat
     1441 ctacacatac aaagtatatt cttgaatcat tagaatttcc aagtactata acatcatttt
     1501 tcttacctag aacttcaaca ccagttagaa ccaaactacc agctactaca gtaacttgat
     1561 tatttatatt tgacatataa agattatcta ggataccttt attacaacag caatcatcac
     1621 aatcgcagtt gcaattacat tctttgtaat gttttttatc aagatagtaa cctaaagatg
     1681 ctatgaaatc ttcataatct gcaggtgtaa tatcaaatgc aacagcttta agattacaca
     1741 atgatgctaa atctacgttt gctaatggag tagatgcagc aagtataaaa tcaattatag
     1801 cagcaagtat tgcatctaat atagccccaa ggtctatcgt aggaggaata actgcatcaa
     1861 taagtgcaat taattcagct actcctggta tagttcctat agtattaatt aatccctcaa
     1921 gagttaaagg gactggaata ggataagata ctctaccagc tatatctatt aaatcacagt
     1981 tacaagcaga aaatctttca aaagtaccat caagtccaga taaattatct tttggaggag
     2041 ctgaaagatc tatacctacc aaattagcac ctactataaa gaaatctgag ataaaagcaa
     2101 attgattaaa gtttacaaaa ggtcttagag catcatatct tagaagttct aatgcttctc
     2161 tcatactttt tttacaacaa cattgtttta ttttaggttc tttatattta tttggataat
     2221 tatagtatct atcagctact tgataatttt catagtatct ttcatttgta tttggataat
     2281 cttcctcata ttcttgtgta aatctaaagt cctctctaca tttattattt tccactaaat
     2341 acactccttt tatattttaa tttaaatgta tagataatta agcttatcta tatagtaata
     2401 ttatatttat aatgtaaaaa aatgttacaa aagatattat aaacgaataa aaaataataa
     2461 tttgtcagat aaacttgtaa atttttatga ataatccatt ctaaaacaaa aaactgtgta
     2521 taaaaaaggt ttatatgtta tatttatata aatataacat atgaacgaga aaggagtggt
     2581 ttgataaatg attgaggtag ataaattgtg taagtctttt acaagggttg taaaagatga
     2641 taagaataaa agttcaataa gaaagctaaa gaaagtaaaa actaaaaaag aagaattttt
     2701 tgctgtaaat aatgtttcat ttaaagttat ggaaggtgaa attgttggaa tattaggtcc
     2761 aaatggtgca ggaaagacta cattacttag aatgttaggt ggcatattaa caccaacatc
     2821 aggaagtata aatatttctg gatatgatta ttcaatagat agaaattcag caaaaaaaga
     2881 aataggttat ctctctggaa atacaaaact atatggaagg ctttctccga gagaacttct
     2941 aacaactttt gcaagtctat atgagatgtc aaaagaagat atagaagaat caattgaaaa
     3001 tattgtaaaa ataatggata tgagtgaatt tatagataat agaattgaaa atctatcaac
     3061 tggtcagact caaagaacat ctatagctcg ttgtttaata cattctccaa aagtatatat
     3121 ttttgatgag ccaacactag gtctggatgt tttaagtagt gcatctatta tagattttat
     3181 gaagaatgaa aaactaaggg gaaaaactgt tctttactca actcattata tggaagaagc
     3241 agaaacttta tgtgacaaga tttttatgat tcacaatggg aaaataattg catcaggaac
     3301 accacagagt ttaaaagagg aatctggagt aaataatttg agagatgtat tcataaaact
     3361 tgccagaaag gaggacattt aaatgagaag taagattgta aaatacatat ttaaaaaaga
     3421 aatgttagac atattgagag ataaaaagac tttatttatg atgattgttg tgccacttct
     3481 tttatatcct ttacttatgt tattaatgat tcaagtaatg aatatgtctg caagtagtat
     3541 gaatagtaag gacattaata tagcttttaa taataaacct aataaggtgt tagtatccat
     3601 gattgaagat gataatttag aaaaagatac taaaaatact tcaaatgata aaggcaaaat
     3661 aagagtgtta gatgtagata actataaaaa gtcattagaa gaaaataaaa ttgatgctta
     3721 tataaagatg gaagaaaaaa aatcatctat aaattatcaa atatatataa attcttctca
     3781 agaaaattct tctaatgcac taaaaagaat tgaaactgtt ttggataaat ataaaagatt
     3841 taccatagaa aaaactttat ctgaaaaagg attagatgta caagctactt tagaaccaat
     3901 aacatatagc actgtagatg ttgcaaagac ggaagaagtg gcaggatact ttttaggtca
     3961 gatacttcca tttatattaa taataggagt tttactaggt tcaatatatc cagcaataga
     4021 tgttatggct ggagaaaagg aaagaggtac attagaaaca ttatttacac tgccaatatc
     4081 aaacttagaa ctggttatgg gaaaatatat ggcagtttct cttagtgcaa tagtaacagc
     4141 aattttaaac attttatcta tgggactgac tatggcattt attttagtta gtggaggtat
     4201 tagtagccaa tttggctttg gaaactttaa ttatggagac ttagtcttac caattataac
     4261 tactttaatt tgcatatgtc ttttttcaat ggtagtttca gcaataagta tgtgtgtatg
     4321 ttcattagca aaaagtttta aagatgctca aaactacata acacctctta tgttaattat
     4381 tatgatacca tcatatgttt ctatgatacc aaatatagta ttagatagag tgacagctgt
     4441 aatacctgta gtaaatatat ctttacttat aaaaagtgta ttatctttta aaagcgattt
     4501 aaatttaata gccttagttt tagcatcaaa tgtagccttt gttatattgg caatcatact
     4561 actgtctaag atgttcaatt ctgaggaaat actttttggt aatagtaaga gtttttcttt
     4621 cttagagaaa agaagtaata taaaaaaagg gtctatacca actgtaagtg atggtgttat
     4681 tttatatgca gtaggattag ttctattgat ttatgttggt tctttagttc aaattaaatt
     4741 tggtatggca ggtattataa tgacacaatt catgataatt tctttaccat tattatttgc
     4801 atactatatt aaagctgatt ttaaagaggt atttagcttg acagttccta aattaaagca
     4861 tgtgtttggg agcattgttc tttggattgg tggatacata cttattatga ttataacaca
     4921 gctaatttta ttcttatttc ctcaaaatat ggaagttgct gaaagtttaa ataaggctct
     4981 ttttatcaaa gatagtgttt tactgaattt actagcaata gctttattac cagctatatg
     5041 tgaagaaatg ttttttaggg gatttatatt ttcttcattt agtaaatcaa aagataaaaa
     5101 taagtctatc aaactcgcta taatatgtag tggggtacta tttggtatta tgcatatgga
     5161 ttttattaga ataattccaa catcaatatt aggtattata tttgcataca gtgtgtataa
     5221 atctggttca atatttgtat caatgttatt gcatttttta aataatagtg tagcagttgt
     5281 gttaaatcat tatacaactg gaaatataac caacttatat aaatttgttg aagttgattt
     5341 ttcgaactta aatgtgctta aatttgcctt aattcttcta ataagtctta tattaattat
     5401 gttaggatta agagttttag atag
//
LOCUS       DAYAOX010000232         5290 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.233, whole genome shotgun sequence.
ACCESSION   DAYAOX010000232 DAYAOX010000000
VERSION     DAYAOX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.233"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(53..1360)
                     /locus_tag="ACGI57_003239"
     CDS             complement(53..1360)
                     /locus_tag="ACGI57_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HFG9718291.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(1785..3560)
                     /locus_tag="ACGI57_003240"
     CDS             complement(1785..3560)
                     /locus_tag="ACGI57_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9718292.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLERMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(3557..3694)
                     /locus_tag="ACGI57_003241"
     CDS             complement(3557..3694)
                     /locus_tag="ACGI57_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718293.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(3772..4341)
                     /locus_tag="ACGI57_003242"
     CDS             complement(3772..4341)
                     /locus_tag="ACGI57_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFG9718294.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(4525..5160)
                     /locus_tag="ACGI57_003243"
     CDS             complement(4525..5160)
                     /locus_tag="ACGI57_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9718295.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         1619 a          967 c          516 g         2188 t
ORIGIN      
        1 ttatttatgc tatttttgtt atacctcata atacaaaaca tattataaag tactagtcaa
       61 ctttattcat atcattcatt acatttaaga ttatctcatc tgttatagac atacctttgt
      121 cacttactct acctaagttt tgtatacttt gctcaacact tgaaccaact ataccattta
      181 gtggtggtac aaaacaatct tgcttagcta ttatagcact ttgtatagct gcagatgctg
      241 cagaagataa ttttaatgca caaccagctt tcgcaccgtc acatatcatt ccacttaagt
      301 cagctatcat attttctata gctccttcta tttgcttttc agtaccattc ataagccaag
      361 agataccagc agttgctcca gtagaagcag caacaccaca tccacataca gcagataatc
      421 ttcctgtata gttcttaata taaccagtta ctagatgaga tatagctaaa gcttttgcta
      481 gtctttcatc agattgtggg aattttttat tataagcaac gattggaaga attgctgtta
      541 atccatgatt tccacttcca tttgaactca taacaggcat ttttacccct gccattcttg
      601 catctgaagc tccagcagtt aacatcattg cataatttat aacatcatta cctaaaagac
      661 cttcttctat agacttttta attccatacc cgacaccaat accagttttt tgttttaatc
      721 catattcagc catctcttca ttcattttta ctccatctag taaaaattct atatctttaa
      781 aatcaatttc ttctacattt tttattaatt cttgtatagt tactgtatcc attaaacttt
      841 cagctttttc tgccgcatca tttgaagctg atacttttgg ttcattgtct aataatactt
      901 ctccatcttt ttctaagaac gtaaaattat catgtttagt tcttattctc actttagcaa
      961 tatgattttt tccttttaac acaacttcta taaatacttt ctctcttgta tcagcaggtg
     1021 ttattgatag tggagtatta tccatataat cctcagctat tttaacttcc tcttttgtta
     1081 gagtttctaa cacacttaat ccattttcag aatgtccacc tactatacct agagctgctg
     1141 caatttttaa tcctaatctt tcagtacctg gtatacctac acacatacca tttttataga
     1201 tactaggact aactaacata cgattttcaa ctatttcttc tcctaataat tcctttgcct
     1261 ttgcacatgc tagagctaag gctactggtt cagtacaccc tactgctggt ttaacttctg
     1321 cctttaacat ttccactaat tcttttctta tatctctcat tatatataaa cctcccaatt
     1381 aagtttagat ttagtactca aataattagt taaataattt cttatctata tttatagcaa
     1441 aatacatgcc atatttttta tatcttttta tatttttagt cttatattct ataaccattg
     1501 gtatgactgc ctctatttat gtattttttt tatttgtata gagtatttat attctttaaa
     1561 atggagaaac atatgacaat ttcgcaaaaa gacaaaaaaa agtctcaaaa tgagattcta
     1621 tatcattttg agacggctca aatatattat tttttaactt ataaaaacca ttttttaatt
     1681 cataaaaaaa gattctgcaa aacacttata gtataagttt aaagctttct ttaaagttat
     1741 gaatttagaa aaatttaaat tttatatagc tttttattat attactatga aattatattg
     1801 tatttttcaa tttttctata taatgtagct cttgatattc caagtgcttt agctgttaat
     1861 tctttatctt ttttataatc tttatactta tttaaagctt ttattatctc aatcttctct
     1921 agttcatcaa gtggaattat cctatcgctt ttaacttcta actcttctac agaggtattc
     1981 ttaatttttt gaggaattac attcattgtg attattgaag aagaagacat attcacacta
     2041 tattcaataa tattctgcaa ctctcttata ttccctggcc acttatattc catcatagca
     2101 tcaacaaccc tgctatctat gtcaataaca tttttatcta atttttttga atactcttta
     2161 accatatgct tgattataag tgggatatct tcttttctct ctcttaaact tggtaatttt
     2221 ataggtataa catttaatct ataatataaa tcttctctaa aagtgccatt ttgaaccatt
     2281 ctttctaaat ctttatttgt agcagcaata attcttacat ctattgaaac atttgattta
     2341 cttccaattt tattaagttc cttttcttgt aatactctca ataactttga ctgtaaatgc
     2401 aaactcatat ctcctatttc atctaaaaat atacttccct tatgagctac ttcaaacatt
     2461 ccaagctttc cacctttttt agctccagta aaagctccct cttcatatcc aaataattca
     2521 ctctctaata aattatccgg tatagcagca cagtttacag ctataaaagg attatctact
     2581 ctattgctat ggttgtgtat ggctcttgca aatagctcct tacccgttcc actttcccca
     2641 gtaataagta ctgttgatgt tgatttagaa gctattaggg cttccttttt agaatttctt
     2701 atagctacac tttctccaat tatgttttcc aattttattc tataatcttt attcatctta
     2761 ttgtagtttt ttatagcatc tcttttatct ataaaatcaa taacatatcc ctttaactca
     2821 ttttttaata ctattttatt gatgttgtat attcccttcg aatcatatcc gtatctttta
     2881 taaaaaaaac tacaggattt gtccttctta aagttattta tagaaggttt ctttataaaa
     2941 tctaactcga caaatatgtt tttgccagtt atactatctt ttatattgaa aagctcttta
     3001 aattttgaat tatatttatc aatttttcca tccacatcaa tagacacaac agctttgtcc
     3061 atattgttta atagtgtctc taattttttc ttttctaatt caagctcata agttttaatt
     3121 tgtgctttta atttattaga tattaggtct gccattttgc ctaaaaagtt tataagacca
     3181 cttttattat tttctataat ttgtgcttgt tcttttgtaa aggctataag gcctataaca
     3241 ccatgagaat cgccatcaca tataatagga caacacacct ctgcaaactc tacacaagcc
     3301 ccacttttat agcattccct acatatatca ctactctttg ggtcatcaat tagtattgta
     3361 atctgttctg taagtgattt tttaaaagcc gtatatttat ctactttctc acctattttg
     3421 tctatatata ctcctgtacc tgcaatccta aataactcct tatttactat agttacatct
     3481 atattgagta ctgatactat agcttcagct atattttgaa tatcagattt tatatattct
     3541 aaacctatca taatcatcat tccttatttt tctttagcat aagtatttgt cttatttctt
     3601 taagaatcaa gtatatctta tttaaagtaa attggattga catcaggatt acaaagagca
     3661 taacaattat tattaactca taatttattt ccatctaaac atctccttat aacaaaagac
     3721 taggcttaac ctagtctttt atattatatc catatttata ttttaactca tttatcttct
     3781 ttttcccaca taaatatatt ttaatttatt tgactcttta atcataccat caaagtttaa
     3841 tcttgaaaat atattaaata cttcatgtgg atagtgaatc tttgcaacag aaccacaaaa
     3901 tttatgttta agatagttta acttaacttt taaatttttt aaatttaaat cctctaatat
     3961 aacctctcca ttgcccttta acactctttg tatttcagat gaagcttttt cacaatctgt
     4021 aataacatta aattggtcaa ctattattat cttgttgaac tgattatcat caaatggtag
     4081 aaactctaac ttacctttaa ctacattgca attttcatta tacactagag aataacagac
     4141 atcttcataa ttactattct ccagtatagt tacagaagtt ccaagacctc tcaatctctt
     4201 gcctaggttt cctatatgac caacaagcaa tattttatca cctacattat actcaattgt
     4261 atccaaaaaa cattctaatt catcagatgt atatttagct ttaactggct gtttatttat
     4321 ctgacataat atgcttttca caatgcttca cctcttcatt tcctcaatta taaattatgt
     4381 acagattaca ttataataaa tggcgagcaa ctattcaaga gatatttcat tagtattttt
     4441 ttaaacaatc tttatacatt ttaatatgat attataccaa atttattagg attttattca
     4501 taaaaatcag tctatttttt atgtttattc attgttattt tcatccaatt tttttatttt
     4561 cgatatggat acttcatatg ccattttttt gaccacatta ttttcatcta tttttttctc
     4621 atattctcta ctttgaaccc taccccaaag ctgtattcta tcaccaacct ttagattctt
     4681 tgcaaattta gcatttctac cccaaactat tgatggtata taatcagatt tattataagg
     4741 tctgtttata gcaactaata aatcggttat ttcccttcct aaaggtgtct tcctatacac
     4801 aggttcctta catacatatc catctaaaaa tatactatta gggtctttat tttcctcgtc
     4861 tatacttttt atttctctga caaacactgt aagcactaat ctatttttat tatttaatgt
     4921 tttgttatat gatcttaatt ggcctacgac attgactaaa ttttgtttat caaggtcaat
     4981 atcttggaga agtctttctg atacagtcat tggaagtata tcaaaagaat cgcttagtct
     5041 gtttattttt attttcatat tataaaattt ttctccaaat atctcatggc taaattctag
     5101 cttattgtct aactcccctc ttaagtttac tacgttattc tcatctttaa cagctatcat
     5161 attttatccc tccacaattg ttaattctta tatttctggt atcagcttat ataataaata
     5221 tatttataag ggatgtattt tattacaaat ctttgattgc tgtttaaaag tttttattta
     5281 gttataaatt
//
LOCUS       DAYAOX010000233         5265 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.234, whole genome shotgun sequence.
ACCESSION   DAYAOX010000233 DAYAOX010000000
VERSION     DAYAOX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.234"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(24..329)
                     /locus_tag="ACGI57_003244"
     CDS             complement(24..329)
                     /locus_tag="ACGI57_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718296.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            complement(1486..1704)
                     /locus_tag="ACGI57_003245"
     CDS             complement(1486..1704)
                     /locus_tag="ACGI57_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9718297.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(1705..2112)
                     /locus_tag="ACGI57_003246"
     CDS             complement(1705..2112)
                     /locus_tag="ACGI57_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718298.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(2198..2686)
                     /locus_tag="ACGI57_003247"
     CDS             complement(2198..2686)
                     /locus_tag="ACGI57_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HFG9718299.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(2891..3988)
                     /locus_tag="ACGI57_003248"
     CDS             complement(2891..3988)
                     /locus_tag="ACGI57_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFG9718300.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(4361..4531)
                     /locus_tag="ACGI57_003249"
     CDS             complement(4361..4531)
                     /locus_tag="ACGI57_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718301.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(4703..5146)
                     /locus_tag="ACGI57_003250"
     CDS             complement(4703..5146)
                     /locus_tag="ACGI57_003250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9718302.1"
                     /translation="MIEIKTIEINKKSRIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         1751 a          828 c          464 g         2222 t
ORIGIN      
        1 caattttata gtagataatt tagttaatta gttcttaaat gtttatcttc tttagtaaat
       61 ttttttctta ttaagaaata tgcaactatt acaacaagtt ctattgctaa aaatacacca
      121 taagtagcta tagaatctat gctatctatg ccatttctaa agaaaattaa agcaattcct
      181 gctgctataa tacctacagc atttttccct atagaatttt gaccaaaacc ataaaaagaa
      241 gggaaatttc cttttatttc aatgaatgtc aagattaatc caatagctat aaatagtact
      301 cctacaactt ccataatcat gctaggcatt ttgacccctc cttattaata attaactact
      361 actataaaaa tatttattat ttttataatt atattatata tgcgattaca tgtttttgga
      421 agtatatttg gaagtaattt catatttatt taacttaatt tttttgtgat aaaatacttc
      481 taatttttat atagatatta ttataaattt tctagtttat ggagtcattc attctataat
      541 tcacaaatat gtatttttta atctattaaa gtattcattt tctggcttat aagcatggac
      601 ttcttatcta taagcttggt ttttcatcta ataagttatt tattctttaa tctatgagct
      661 tgatttttca tctataagtc atttattttt taatctatga gtttggtttt tcatctataa
      721 ataagtcatt ttttaatcta taagtttagt tcttcatcta taaatcattt attttttaat
      781 ctataagttt ggtttttcat ctataaataa gtcatttatt ttttaatcta taagtttagt
      841 tcttcatcta taaatcattt attttttaat ctatgagctt gatttttatc tataattaat
      901 tcattctcta atctattagc ttggttttta tttataagtc atttattctc taatctatta
      961 gcttggtttt tacctataat taattcattc tctaatctat tagcttggtt tttatttata
     1021 agtcatttat tctctaatct ataagcttac ttcttaatct ataaatcatt cattttttaa
     1081 tctattagct tggtttttac ctataaatca tttattctct aatctataag ctttcttttt
     1141 catctataaa tcattcattc tttaatccat tagcttggtt tttcatctat gagtcattta
     1201 ttctctaatc tattagcttg gtttttattt ataagtcatt tattctctaa tctattagct
     1261 tacttcttaa tctataaatc attcattttt taatctatta gcttggtttt tacctataaa
     1321 tcatttattc tctaatctat aagctttctt tttcatctat aaatcattca ttctttaatc
     1381 tatgagcttg gtttttcatc tataagtcat ttattctcta atctataagc ttggtttttt
     1441 acctataatt aattcattct ctaatgcata agtaatacat gaaatttaaa tattattttc
     1501 ttcaataaat tcaaacaact cttctacagt tgtatcaaat accttagcaa tatccattgc
     1561 cagttttaaa gaaggattgt agcgaccatt ttctaaatgt acaatagttt cacgtcttac
     1621 tcctactgac tttgcaagtt caccttgtgt cataaggttc ttttctctat aggatttaat
     1681 ttttgtcttt agtaatactg acacctaatt gtatatcccc tttctgtcaa aatatataaa
     1741 tagaattaac ttcaacaagg attgaataaa caaaatgact aataaagaca ttcctatatt
     1801 taaatttgac acatttatgt ccaaagttac aagaaatata gatactatag ctacagaaaa
     1861 aaacaatact tttattgcaa tatcattaac tcttgatagt atacattttg atgcctcatc
     1921 aattaaatca tcatactttt tattatagac ccactgagct cccatagcta atacccaaat
     1981 aattatagga agatattcaa tactacttcc tttaaaaaaa tacttattta ctaatgtagc
     2041 tccaatacaa taagaaattc ccaatatttc tgaaataagt gttgatttaa taccagtttg
     2101 ttgtttagac atataaaatc ccccttaaaa agttatgtat ttataacacc tcttgttata
     2161 aatatataac ttttttgaat aaatttcaat aaaaatatta ttttgcaaca aattctttta
     2221 ttaattttac attagataga ggcactttag atgtcactcc ttgcaatgtc aaatcttcta
     2281 tattaacttc atttaatttt gaacacagtt ctaagtcaaa tattctttcc cagcttacct
     2341 tcttttcaat ttgttcatct tcataaagct ctacagtaat atccattaaa ggtaaatgcc
     2401 ataaatcaaa ttctgataat aacacattgt cttcacttat ttctatttca agtaaaacac
     2461 ctttttttcc tttttctaaa taaccactac atcttaaatc aggtctcttc gtccaaaccc
     2521 atatcatact atcatcaaat tcatatattc tatttgtcat ttgactatac atccatttgt
     2581 atggaatatc aaagtcttct aatataaaag aataatcatt tgatagatat ttattgtttt
     2641 tattgttatt ccaaaattct aaagtctgta tagtataaaa tttcatatat aaatcaaccc
     2701 ctttgttttt ctataagaag gagatggttc aaacttttat tttgaattat ctcctttatt
     2761 tatacacctc tttgtaccta ttatttatat atctctttat atctaccata tttaaataac
     2821 aaagttttat attaatttaa atttaaaaga ggaagctttg tatttatcta gtgccatctc
     2881 aatcatagta ttagttttga ttatagactt tttcaacctc tgatttattc aaatcatcta
     2941 taatatcgca tatttcactc aaattgtatc ctttattttt taattttatg atgatattaa
     3001 atttctcttc tcttttccct tttgcaatac ctttttcaat accttcttga aaaatttctt
     3061 ctttaaaatt tctactttta ttcataatat acgcacctct ctttaaattt aataaatagt
     3121 cctcattact acctaattct tttaatctat caagtgctct tttaataaca taatccgttt
     3181 tagatagtat tctaatatct atatgagaag gattatcaat aagtttaccc catattagaa
     3241 ctctgtttgc tatacaactg gatttacgaa tttttggaag ttctataaaa tataatgcag
     3301 ttttactaga ataaatatct ggatgcccgt ccattgttaa tctcaggcaa tttacataat
     3361 cttctatgtg attatatttt acatgattta gtatatgtat tccaacagtt tttccaattt
     3421 ctttatattc tttaccttct tctaactttc taccatgtac acgactaaga taatattcag
     3481 ctctttccaa ataatcctct tcaagagctc tttgcatctc aatatttata tggttacgtt
     3541 cttcatcttc agccaaaata tcaagtctag gtcttttctc accaatgtaa ttaattgttg
     3601 ggtcggtttc aaccttaatt attcttttta tttgagtgaa ctgtttgata ttcctattag
     3661 catatggagc atctttgagg atatcattta taaaatttgc aagtaaatcg taatcattat
     3721 ttaaagtaaa caatgattta aatacctcat catactcagg cttagcatac ccatcttcag
     3781 ttacaacttc tccaacattt tcactagtca atatttcttt tggaattaaa tccttgattt
     3841 tatctaattt ttcattagaa aaatcacctc tactaagtat gtcatttaat tttttggtcc
     3901 attctctgtc cttttcttca attatagttc gtttatactc ttttgatgtt ttaataatct
     3961 tagatttttc ttctaatttt tctttcatat caacacctct tttttattat tacccatttc
     4021 ttcataccaa agtctgaact tctaagtaaa atttgcatta ttgaaataag aaccttataa
     4081 acttatatat cattactaaa gttaagactt taaagagaag cacttagatt acaatagatt
     4141 ttagatagct ttctttttaa aactattgtt gataaatgaa tgaatataag ggtatgtatt
     4201 aaaatagaga tactcatctt tttcattgtt taaataatga aaatatattt tatttaatac
     4261 aaaaaagagt gccttataaa ctaaaagttc acttcgagac actcccttac attttgtttc
     4321 ttaaacttta caatattaca tacttattta ttaatttaat ctatgcatac ttaacccaat
     4381 aataaagtac atatatccaa ctcattaagc catgaaaaat agcccatgga atagatttcc
     4441 atgcagtata agaaataatc attgctaaac aagaaccaaa agaaataccc tttttaacag
     4501 ttgattttat agtattatta tttgaatcca taattaatcc tccaaaattc aaaataaaca
     4561 agttattata tatcttattt gaatataata caatataaca gcttctaaat aaattatact
     4621 cttattatac ataatttata aattataatt ccgataatta tatgaattaa ttaattatat
     4681 taacctaaaa atattatcct atctaaaagt tatatgatag atacaacatg tcaacacatt
     4741 gtaattctcc ttcaaagatt ggttctggat aattatctac aaaaaagttt ttaattttat
     4801 gtgaataaac aaatccaaac ttctcataaa aaggaactcc gctttcagat gtaccaacaa
     4861 ccattgatga atatttttta ctataaacat ctaaaagatg ataaatcatc tttgaggcat
     4921 aacccatatt ttgaaaatgc tcatatgtag caatgttttt taattcacat atttctttgg
     4981 aaatttcatt tacaactgct atacaagtaa gttctttatt atcatacaat gcgtacatag
     5041 ttcctttatt gagatattta tcaatcattt cttcacatgg gtcggctaac attaaaaggt
     5101 caattcgact ttttttattt atttctatag tttttatttc tatcataagt tctccaccct
     5161 cactttattc aaaccttaat ttaacaaact tcttacagta cttatttaat tcattttgat
     5221 atatattttt actttttaaa atatagtgat aattccctag aatat
//
LOCUS       DAYAOX010000234         5254 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.235, whole genome shotgun sequence.
ACCESSION   DAYAOX010000234 DAYAOX010000000
VERSION     DAYAOX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.235"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            56..2638
                     /locus_tag="ACGI57_003251"
     CDS             56..2638
                     /locus_tag="ACGI57_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFG9718303.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSSISMDLYTSSSLGIPKELMGSNTNNIYTLSNTSKIDTLNKNHSNTA
                     VTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNIDCYLNA
                     FNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVTEYCPMG
                     VVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLDNLYELE
                     EIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKRATDLYKKLDEIGTVAGHYY
                     KGVE"
     gene            2742..3320
                     /locus_tag="ACGI57_003252"
     CDS             2742..3320
                     /locus_tag="ACGI57_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFG9718304.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            3354..3830
                     /locus_tag="ACGI57_003253"
     CDS             3354..3830
                     /locus_tag="ACGI57_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFG9718305.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            4348..5169
                     /locus_tag="ACGI57_003254"
     CDS             4348..5169
                     /locus_tag="ACGI57_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9718306.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
BASE COUNT         2123 a          503 c         1055 g         1573 t
ORIGIN      
        1 ttaagttata gatataatta tattaaccta acaagtataa atagaggaga aaaatatgaa
       61 aaaaatagaa ttacttgcac cagtaggctc ttttgattca ttaaaagcag cagttcaaaa
      121 cggagcaaat gctgtgtatt taggtggtaa agactttagt gctagagcat ctgcaaataa
      181 ctttgataga aatgagctga ttgaggctgt taaatactct catataaggg gagtgcaagt
      241 ctttgttact gtaaatacat tgataaaaca gtatgaaata gaagattttg tagagtatgt
      301 aaaattttta tacgatatag atattgatgc tttaatagta caggatatag gaatggcaag
      361 acttataaaa aaactattac ctgactttga actacatgca agcactcaaa tggtagcaca
      421 ttccttagaa gatgtaaaat accttcagag tgtagggttt gatagagttg ttttagcaag
      481 agaattaaat gttgatgaga taaaatggat ttgtgaaaat acaactgtag atatagaagt
      541 ttttgtacat ggagctttat gtgtatgtta ctcgggtcaa tgccttatga gtagtatgat
      601 aggcaatagg tcgggaaata gaggtaggtg tgcacagcca tgtagacaaa agtatgagtt
      661 gatagatata aatacaggag aagtagtaaa aagtaatgga gattatttgt tgagtcctag
      721 agatttaagt actatagaag atttggataa aataattgaa gcaggtgtat tatctttaaa
      781 aattgaaggt agaatgaaaa agccagagta tgtagctact gtggtttcag gatatagaga
      841 agcgattaat gaatttgtag ataagaaaaa aataagtatt tctgaggaaa taataaataa
      901 tttgtatacc atatttaata gaaagtttac aaaaggatat atattaggtg aagttggtaa
      961 ggatataatg aactctaata gacctaataa tcaagggcta tatattggaa aagttttaga
     1021 ttataataga aagaacaaaa gacttagagt aatgctagaa aatacgctta agaaaggtga
     1081 tggaattaac ttaggtggag gtactattgg aagaataata aaggatggag agataactac
     1141 aatagcacat aaaggggatt tgatagaatt agattttgtt ggagaagcaa agagaaatca
     1201 gcttatatat aagacttctg acagcgaatt gatggataga gtacaagcta catttcagca
     1261 agataaagag ttcataaaaa ctaatatacg tgcaaaagtg agtattaaac taggtaaaaa
     1321 gccagagctt tttataagtg atttttgtgg aaataaatcg gtggtagaag gagacaaaat
     1381 agttgaaaaa gctataaagg tggctttaag taaagaaaaa atagaaacac aaattcaaaa
     1441 gcttggaaat acaccatata agttaaaaga gatagaaatt gacctggatg aaggtgtaag
     1501 tatgcctata agtgtgttaa atcagatgag aagggattgt attgagcttt tagatgaaga
     1561 aagaataaag ataaaagata gaaaatttaa gaagaataga ttaaaatacc agccaaatag
     1621 agtaaataaa cagaaaaaac ttgatacagt tcctaagatt agagttaaag ttaagaactt
     1681 ggagcaatta gaagcagtat taaattataa tgtagattta atctattatg aagatataaa
     1741 tacattatca aaagctttac aaatggtctc aagtatttct atagattcaa gtatttctat
     1801 ggatttatat acttcttcat ctttaggtat tcctaaagaa ctaatgggtt caaatacaaa
     1861 taacatatat actttgtcaa atacaagtaa aatagatact ttgaataaga accactctaa
     1921 tacagcagtc actgacaaaa aattaatcta ttcagcacca aggattgtta gaaataaaga
     1981 gtacaagcaa tttgagatat tggataatat aaaaggaatt aataaggttc aaattggtaa
     2041 ctggggctct atggaatact ttaagaaaaa agacttaaat atagattgct acttaaatgc
     2101 attcaatagt gaaagtataa accacttcaa agaagaggga gcaagtacag tttgtttgtc
     2161 acaagaacta aacttaaatg aaattaaaca aactttaaaa tatacagatg tagaaattga
     2221 agcagtaata tatggatatg ctcctatgat ggttacagaa tactgtccta tgggagtagt
     2281 tgttagggat tgtaaaaagg acaagagagt tgctaaatgt aaggaaagta gttatgcttt
     2341 aagagacttt acggatgcaa gttataggat aacacaagat atattctgta gaagtacaat
     2401 ttacaattcg aaggttactt gtatgttgga caatctatat gagttagagg aaattggaat
     2461 agatgtattt aggatagatt ttacagttga agatagtgag atggttaaaa aggttattga
     2521 agctcacatt gaggtattga gtaataatta taaacttgga aaaagggcta cagacttgta
     2581 taagaaatta gatgaaattg gaacagttgc aggacactac tataaaggtg tagaatagat
     2641 ataacatgaa aaatatacat gaatatatat aaaaacttga atttatgtgt attaatatat
     2701 aaaaatatca tatatgtgaa aaaacagggg gattggtgaa aatgagagaa gaagctttag
     2761 aaattttatt taagtattta gaaactgata gaatgagaaa acattgttat gctgttgaag
     2821 ctgttatgag agaattagcc aaaagactag agccagaaaa agaagaagag tgggcagtgg
     2881 caggtctttt acatgatttg gattcagata tagttggaag aaagccagga gaaatatgtg
     2941 agggacatgc tacaacaaca gtagaactgc ttaaaaaaga aaacttcggt gacgaagaaa
     3001 tgtatagagc aatacttggt catcatgatg gaatgggagt tacaagaact agcctaatgg
     3061 aaaaagcaat atatgcagca gaccctataa ctgggtttat aactgctata gctttagttt
     3121 atccagataa aaaacttacc agtgtaaaga ctaagtctgt tattaaaaga atgaaagaaa
     3181 cgagatttgc ttcaaatgta aatagagatg caatgagaag tatagaagat atagggattc
     3241 catttgatga atttgcagag ctttctctta atgcaatgaa gaatataagt gatgtgcttg
     3301 aaagtgctga aaataattag ttgagaatat aaagagaaag caaggcgaga tacatgaaac
     3361 ttacaactat ttgttatata gaaaaagatg acaagacttt gatgttgtac agaaataaaa
     3421 agaaagatga tatacatgaa ggtaagtatg taggagttgg aggaaagttt gagcaaggag
     3481 agactccaga agaatgtgta attagagaag taaaagaaga gactggtctt actcttaaat
     3541 cattgtcata taaagggttg ataacttttc caaaatttaa agatgaagaa gattggtata
     3601 tgtttttgta tttctcagat gagtttgaag gtgaattatc tgaaaaagat ttaaatgatt
     3661 gtaaagaagg aaatttaatt tgggttgata atgataaaat attcgattta aatatgtggg
     3721 aaggtgacag gctgttctta aattgggcta agactggaaa tatctttagt gcaaagattg
     3781 tatatgataa tgggaaacta aaagattata atgtaagttt cttagattaa ttaagatata
     3841 gtttttagat taattaaaat gtagtttaat gatatatttt aagaatataa tttaaagata
     3901 ttacttaaaa atgtaattta aagttacatc aaattaaaat gtaacttaca ggttatagca
     3961 aattaaattt attgactaat attaagataa acaatagtag ttgggaaaag gggctatctc
     4021 agtgcataaa gatagccttt ttttagtctg tctacttgac agggacatat ccaaaataga
     4081 ggtaagcctt ttttattgtt gaaatgataa atatccactt gatttcatac aataaagaat
     4141 atctaattaa ctaaaaagtt cacttttaga catctacttc ttttatcttt tactttgaat
     4201 aagttctaat ttaagttcca atttatgtat atttttctcc attaaagtgt agttaaggct
     4261 agtatattgt taaaaaaata aaaattgact tagacaacta tttgttttat actaaagtaa
     4321 attttttaaa aggaggaata atttggaatg atagacataa atttagaaaa atgtgtaggc
     4381 tgtgggatgt gtgaatcaga ctgtcttgta aatgcaataa aagttaagga tgataaagca
     4441 aaggttaaaa atattctctg tattaactgt ggtcactgta tggcaatttg cccaacagat
     4501 gcaatagaaa tgcaaggatt tgataaaaat gaagtaatag aatacaatcg tgaaagtttt
     4561 gaattagaac ccgaaaagtt attgaatttt attaaattta gaagaagtat aagacaatat
     4621 aaagatatag aggtagaaga agaaaaaata aaaaacatag tggaagctgg tagatacaca
     4681 ccaacaggtg gtaatagaca gcctataaga tacatattag taaaagaaaa gctaaaagaa
     4741 gtgaaagaac tggcaataca aggtctttat aatttggcat tagatactga tgacaatgac
     4801 cctgtacgtt ctatctataa aaatacattt aagaaaatgt acaaaagata taaagagaac
     4861 ggaaatgatt ctttatttta tgatgcccca ctacttatgg tagttgtagg agatatgtct
     4921 ctaggtggaa gtgcttatgt agatggagga ctggctgcat ctaatatgga acttatggca
     4981 tattctcaag gattaggaat ttgttataat ggattttttg ttatggcatc aaatgtagag
     5041 ccaaagataa aagaactgtt aggaatgagc gagaacgaag ctgttatcac ttcatttata
     5101 ttgggttatc cagatgtgaa atataaaaga actgtaaata gaaacactgc taaatttgag
     5161 gtgagataga acttaaactt cactacaact tgtaaaatta tgggatatag agtaggtatt
     5221 tagcagggta aatttgttgt aatagacata taaa
//
LOCUS       DAYAOX010000235         5224 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.236, whole genome shotgun sequence.
ACCESSION   DAYAOX010000235 DAYAOX010000000
VERSION     DAYAOX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.236"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            176..1639
                     /locus_tag="ACGI57_003255"
     CDS             176..1639
                     /locus_tag="ACGI57_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFG9718307.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1695..2831
                     /locus_tag="ACGI57_003256"
     CDS             1695..2831
                     /locus_tag="ACGI57_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9718308.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            3067..4182
                     /locus_tag="ACGI57_003257"
     CDS             3067..4182
                     /locus_tag="ACGI57_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HFG9718309.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4334..5203
                     /locus_tag="ACGI57_003258"
     CDS             4334..5203
                     /locus_tag="ACGI57_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HFG9718310.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSRRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         2010 a          538 c          987 g         1689 t
ORIGIN      
        1 tatgattaat actcttgatt ttagaaataa atatttgtag attgtagatt aaagataata
       61 taaataaata gaaggtagat tttttagata tattaatata aagattatat aaaatattga
      121 aatatagctg tttaaattat tgtctataaa tataaaatga atggtggtga gttaagtgac
      181 cgatttagtt aacaccatta atggaattgt ttggtcaccc gtattagttg caatgtgttt
      241 gtgtatagga ttatattttt cattgaaatt aaaatttttt caaataagag atgtaaaaga
      301 aatgtttact ctcttgcttg agggagatag ttctcatgtt ggtatatcat catttcaagg
      361 ttttgctaca tctatggctg gaagaatagg gactggaaat atagctggtg tagctgtagc
      421 aatagcaatg ggaggtccag gagcattagt atggatgtgt ataatggcta cattaggttc
      481 agcaactgca tttgtagaat ctactttagc tcaaatatat aaagatgagc atgatgggca
      541 gtatagagga ggacctccat attattttga aaaaggtctt gggtggaaaa agtgttcagt
      601 tttattttca atagttgcaa taataagtta tacatttttt atgcctggta cacaatcaaa
      661 cactttatat ctagcaatga atcaagcatt tggattttca aaaatgacaa tagcagtagc
      721 atcttctatt gcattagcga taataatttt tggaggaatt aaaaggattg gtttatttgc
      781 tgaaaaagta gtacctataa tgggtggagc ataccttatt atgacattag taataattat
      841 aacaaatatt agtttagttc cagaggctat tgtaacaatg tttaaatctg catttggatt
      901 tggttcagtt tttggaggaa tgctaggttc agcaatatct tggggagtaa aaagaggtat
      961 atactcaaat gaagcaggag aaggttctgg gacttatggt gcggcagcag cagaagtaag
     1021 tcatccagcc aaacaaggat tagttcaagc attttctgtt tatgtagaca cattattaat
     1081 atgtatggct acaggtatta tgattgtaat aacaggaatg tataatgtta caggtgtttg
     1141 cacagcacta ccaggtgtag aaccgggacc acaatatgta cagagtgcaa taaatacatt
     1201 acatccacaa ttaggtgata tttttatagc aatagtttta ttattattta catttacaac
     1261 tttattagcc ttctcatata tgatggagac taatgtatct tacttagtaa ctagaattaa
     1321 agcagaaaaa catgaaaagt acataattaa tgtctgtaga tttatgctta tagcatgtgc
     1381 agcaatgtgt tgttttatag aaccacttac aagctgggca ttaggtgata tatgtatagg
     1441 tgtattaaca tatataaatt taattgccat ctttacacta aaaaaaccag ctataaaggc
     1501 atattatgat tatataagac agaagaagat gggaataaaa aggagtgata gaacttttaa
     1561 ccctatagag ttagatataa gaaatgctga gttttgggaa aatagatact taaaagagga
     1621 taagcatgag ttaaattaaa gttataataa aatataaaaa ttactaaata aatttagtgg
     1681 gaggtaagat tggtatgaaa gaaagaataa tacaagtggc tgattcaaag aaagaaaaaa
     1741 ttctagggct atgtcaatct ttatatgatg aaccagaaat tgctttgcaa gaatataaat
     1801 ctgctaaaaa aatatcagaa tttctaagag aagagggttt tgatgttgag gaaaacttgg
     1861 caggtatggc tacagctttt aaagcaacca agaaaaatgg agatggtcct aaaatagcat
     1921 ttatagcaga atatgatgct cttccaggca atggacatgc atgtggacat catttaattg
     1981 catctatggg agttggagct ggaatagctc tttcaagtat actggatact tacaagggag
     2041 aagtaagtat aattggaaca cctgctgaag aaactggaga tggaaagcct tacttgatag
     2101 aacatggtgt atttgatggc tatgatgcag caatgatgat acatccaaat tcaaaaactt
     2161 gtgtaacacc tgaaattata gcaataggag gtctagattt catatttact ggaaaagcat
     2221 ctcatgcagg agctaagcca tataacggaa taaatgcttt agatgcagta gtactactat
     2281 ataataatat aaatgcacta agacaacaat tagtagatgg aacgagaata catggtatta
     2341 tattagaagc aggaactgct gcaaatgtaa tacctgatat gggaaaagta agattagaga
     2401 taagggcaaa agaacaaaat tactttgatg aagtagtaga aaaagtgaaa aattgtgcta
     2461 gaggtgctgc aatagccact ggatgtgaac ttgaatttta tcactttgaa ccaacttgcc
     2521 aaggattaaa tgaaaataaa gttttggtag acatatttac aaagattatg gaggaatttg
     2581 gaatatatga agatgaacaa gtaatgttag gttcaacaga tatgggaaat ttaagtcaaa
     2641 taatgccatg tatacatcct ttgatgaagt tttctgaaaa tggagaagaa ctacatacaa
     2701 aagaattttt ggaggcttct ataaattcat atgcaaaaga tagagtaatt gatggtataa
     2761 aaatattagc tttaacagga tttaatttat ttgaaaatcc agaattattg aaaaaaatga
     2821 aggaagagta gaacttagac tatttttaat tatactcttt aaaataagaa gagtgtctca
     2881 aaatgaactt tttagtttat gagacactct tttttgtatg aaattaaata tatttttatt
     2941 attttaaaca tgaaaaagag gcttatctgt ttttatatga ccttttttaa cttgtagcac
     3001 aaaatatcat gaatgattac aattcgaatg ataaaatcga ttataagata tataggaggg
     3061 gtacatatgg aaactttaga agtaagaaat attgaattga gtggtacaaa ttatgagata
     3121 ggatacagac taggagaatt ggttgctaat atgccagaga taatagaagg tcagattaat
     3181 aaatctaatc tggtaagcaa aaaagaagaa aaagagatga tagagttgtt tgataaatat
     3241 tgtccaggtc ttaatgaaga attgcaaggt tttgctgatg caattcaagt gaattgtaat
     3301 caaattttat attatactat gacttatctt aaaccaggtt gtagtcaagt tgcattagca
     3361 ccagaattaa ctgaaaatgg gcatgtacta tttgcacgta atttcgattt ctcacacaat
     3421 atggaggatt ttgttttatg taaaactaag gtaaatggaa aatatgcaca tataggtact
     3481 accattatgc aatttggaag aggtgaaggt atgaatgaat gtggcttagg tgtaagtcag
     3541 tcatcatgtg gtattcctgt aggcaattca gatggtttaa gaaaaccagc aattgtaggt
     3601 ttacagtttt gggctgttat tagatatttg ttagaaaatt gcaaagatgt agatgaagca
     3661 ttagaatatt taaaagatat gccaattgca tacaatatta atttattgct tgcagataag
     3721 tctggaaaca ttgcacttgt ggagacttta gatgggaaaa aagaagtaaa tataatcaac
     3781 agtttagaaa gtaaaagaga atatttttta cactctacta atcatacaca tattgataaa
     3841 cttcataaat tagatccaca atcaatgaaa aattctattc atagatataa attaattaaa
     3901 gaatatataa ataagtctaa aaaaattgga gagaaagagt taatgaattt attgtcttca
     3961 aaatatccaa atggcttatc ttgtaattac tataatgatt tttttggaac tttaaaaagt
     4021 atagttatgg atttaaatat aggaaagttt aatattttat ggggtggact agagaataaa
     4081 tgggagagct actatttaaa aaatgacatt aaatctataa cacagaggat aaacataaat
     4141 attgaaaaag caccatctga cttttttgat ttcattgaat aaaaaatgta tttaattatt
     4201 ctaaataatt aggacatata aatcaactaa aagtttttat ttatcgtatt gaattagtta
     4261 taatatttta gtatagtaaa aaatagtgct attttacata tttaaagagt ttaagtagaa
     4321 gagaggtgcc atattggatt acttaaagta ttttgaaaaa gctgtaatgt atattgagaa
     4381 taatttacat gaaaatataa ctgtaaatga tgtggcaaaa gaaactggat attcttatta
     4441 tcatttaaca cgcttattta agtctatgtt tggtgaaagt gtaggaagtt atataaaaaa
     4501 aagacgatta gtaagtagta caaaagaact tttatatagt gataaaaaag tgatagatat
     4561 agctattggt agtggatttg aatcttcaga agcatttagc agagcattta aatctatata
     4621 caaggttagc cctatagaat atagaaaaaa tagaattgat gtatttgttg gtaaaaagaa
     4681 aaaattagag ttagatttta tgagacatct tgttggaaat ataacgatta aaccagttat
     4741 aaaagaaata gaaaaaataa aggtaatagg tataaaggat aaagtaattt tagaaaataa
     4801 ttcattacct gacttgtggg agaaattcag aaaagtacat catattgtcc caaatacttt
     4861 gccatcaagg agagtttttg gaatctgtga agcaatctct gaaatacacc tagttagtga
     4921 aagtatggag tttaatgaga taataggact tgaagttaat agttatgatg tgattccaaa
     4981 ttcatttgtt tcaaaaacta ttaaggaagg aaagtatgct atatttactc atactggtag
     5041 cttagataat ttagacaaaa cttatgaata tatatggggt acttggtttt taagttcaaa
     5101 agaagaactt gatataagag atgattttga agtatatgat gaacgatttt taggtcctag
     5161 taatgtgaac tcacaaattg atatttatat acctataaaa taatctataa gaaaactaaa
     5221 agat
//
LOCUS       DAYAOX010000236         5207 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.237, whole genome shotgun sequence.
ACCESSION   DAYAOX010000236 DAYAOX010000000
VERSION     DAYAOX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.237"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            22..798
                     /locus_tag="ACGI57_003259"
     CDS             22..798
                     /locus_tag="ACGI57_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFG9718311.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
     gene            complement(890..1642)
                     /locus_tag="ACGI57_003260"
     CDS             complement(890..1642)
                     /locus_tag="ACGI57_003260"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFG9718312.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1803..2753)
                     /gene="galT"
                     /locus_tag="ACGI57_003261"
     CDS             complement(1803..2753)
                     /gene="galT"
                     /locus_tag="ACGI57_003261"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFG9718313.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3138..4334
                     /locus_tag="ACGI57_003262"
     CDS             3138..4334
                     /locus_tag="ACGI57_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFG9718314.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4669..5121)
                     /locus_tag="ACGI57_003263"
     CDS             complement(4669..5121)
                     /locus_tag="ACGI57_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9718315.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
BASE COUNT         1860 a          788 c          627 g         1932 t
ORIGIN      
        1 aaaatttagg agataaactt tatggaatac ataattttcg atttggaatt taaccaagga
       61 tttgataaaa aactcaataa aactgtatcc aatgaaaagt gtccttttga aattatacaa
      121 ataggagcaa ttaaattgga ttctaaattc aatataatag atacgtttaa tagctatgta
      181 aaacctacaa tttataaaga catacatccc tttattagtc gtatgactaa tattaaaagt
      241 tctgatttta atgattcgcc tactttccct gaggtctaca acaattttat aaaatttata
      301 tcatcacaag accctatatt atgtgtttgg ggagctggag atttgaagga attatacagg
      361 aatatcaact atcacaagct accatcaaat tccttaccta aatcttatat taatatacaa
      421 caacatgcat ctaaatattt caataatcca gcaggaaaaa gcattggcct acaaaatgct
      481 atttctatat tggaacttga tgaaaaaatg tcttatcaca atgctttaaa tgatgcatat
      541 tatactgcta aagtttttat taagatatac aatccaagta tagtcccaga tatatatctt
      601 tatacgagta taaaacctaa aactataaga tattctaaca aaaaaagagt tgattatgat
      661 aagttatttg atgaatttag gaaaatttta aatcgtgaat taactaaaga cgagaaaaaa
      721 atcattaact tagcttataa tatgtggaaa acaaatcaat ttacattaga aaatgttaag
      781 caaagaaaaa acaaatagag tttatttaga taaaaaatag gcatgtagat taaaaaacta
      841 gttaacttta aatttatatc tacagcccaa tttttatcat aattaatttt tattttatct
      901 ctaattcaac tggacaatgg tctgaaccta atatttcagt gtgtatatcc gcacttacta
      961 atctatcttc taattttttg gatgcacaaa aataatctat tctccaccct gcattatttt
     1021 ttcttgcatt aaatctatac gaccaccaac tataaacacc ttctttatca ggattaaaat
     1081 atctatatgt gtctataaac ccactgtcta ataatttaga aaacttctct ctttcttctt
     1141 tagtaaaacc tgcatttttc atattatttt taggattttt taaatctatt tctttatgtg
     1201 caacgttcat atctccacac acaattacag gcttatgatt atctagtttg gttaaataat
     1261 ctataaagtc gtcttcccat ctagttctat attctagtct ctttaattca gattgagaat
     1321 ttggcgtata tactgtaaca aagtaaaaat cttcaaactc tagagttatc actctaccct
     1381 ctttgtcatg ttcttctata tttattccat acattacttt taaaggctct ttttttgtaa
     1441 atatagcagt acctgaataa ccctttctct cagcataatt ccaatattga aaatatcctg
     1501 gtaaatctaa ctctatttgc ccttcttgca atttagtttc ttgtaaacaa aatatatctg
     1561 catctacttc tttgaaaaag tctaaaaatc ctttcccaac acaagctctt attccattaa
     1621 cattccatga tataaatttc atacttatac tcctcatcta aacattgaca cactccccat
     1681 agctaaagat gggggctttt caacaatttt attgtaaatt cttaatttac ttttatttta
     1741 acaatgctat actataattt catctaaaat tactatttac ttaataccta aaaatatatg
     1801 ttttaatcaa tttttaactt ttttgttaaa tcttcagggc tcattgaaga aacatacata
     1861 tttgttccaa gttcaaatcc tccaaaatta tattttcttg ggattatgtg catatgaaca
     1921 ttaaaatacg attttgtttc aatctttgtt ggatgagtgt gaatacataa attaaatggc
     1981 atatatccat tgatattgta taacttctta aacaactttt caaatattgt agaaagctcc
     2041 tgtatattat tatcatttaa ctcaccaaac tctattttat ctttaaatat tattcttact
     2101 tctccactat acttggttgc atatggtata agtaccaaaa aagcttctcc attataaaca
     2161 actcttctat ctaaacttat ttcattttct attaaatcat catacaaaga gcttttattg
     2221 ctgtcataat actcctttga aaccaacagt tcattcgtaa tttcaggtgg aataaaagac
     2281 atagacaata tttgagcatg tggatgcatc aaagatgctc ctgcctttct caagaagttt
     2341 ttgaagatac taacatactc tactttatca tctttcgaca aatctctaaa tctatttcta
     2401 tacataaaaa aaacatcttc aaattctttt tgactcatgt tataaaaact acgattatgt
     2461 ttatcgctct ctatcattac ttcatgaaca ccatatacat ctcttgccat atcatcaatt
     2521 ataggaaatt tattgtagac agattttaca agccatcctt cttcactatc tatttcaaat
     2581 aatgcatcag ttgcatgagc ttcatttcct ttgcaaaatg ggcattcttc atcatattca
     2641 ttagtttctt catcttcatt tgggatatct gctgtatcta aaggtctttt taacctatct
     2701 gtagcaaata taacaacatc attggtaatt ggatctattc ttaattcttt cataattaac
     2761 acctcttagt taatagcctt taatataatt atatgatatt tatcgtatct gttaaataaa
     2821 tttataatat tttttataat tttgcatatt tattattata tattttgtct atagctgaaa
     2881 gattataact aaaaaatcac tatcagtaca tcagaatatt atcttactat aaattaattt
     2941 atgaatacat aatttagtgg cataaacatg ggtattttac tataatttgt taaaagttta
     3001 ttttaaagct atattaattt tttttgatga tttttaccca taagtatgtt attattatct
     3061 tgtaaagatg tcttgtattc aaacatacaa tttacataat acaatatacg tatttaaatt
     3121 aaataggggg tattaaaatg aattattcaa acagagttag tgcaatgcaa gcctctccaa
     3181 tcagaaaact tgttccattt gcacaagcag caaaagataa aggaatcaaa gtatatcact
     3241 taaacatagg acaaccagac ataaaaactc ctaaaggatt ttttgatgct gttaaaaact
     3301 ttgatagcga agtcttagaa tatgctacat cagaaggtat tccagaatta ttagaagcac
     3361 ttcaaaatta ctataaaact tacgatatga attttgaaaa agatgaatta ttagtaacta
     3421 acggtggtag tgaagcttta cttttcacta tgatggcagt ttgtgaccca ggagataatc
     3481 tattagttcc agaaccattc tatacaaact acaatggatt tggtcaatct gtaaacgtag
     3541 aagttaatgc tgttacaaca aaggcagaaa acggtttcca ccttccatct aaagaagaaa
     3601 tattgtctaa agtagatgat aaaactaagg ctataattct ttctaaccca ggaaatccaa
     3661 ctggtgcaat ttatacaaaa gaagaactta atatcctagc tgaaatagct aaagaaaaag
     3721 atttatggat aatagcagac gaagtttata gagaatttgt ttatgatggg cttgagtata
     3781 caagctgtgg taacttagag ggtgtacagg atagagttat aataatagac agtgtttcta
     3841 agagatacag tgcttgtggt gctagaattg gttctatcgc ttgcaaaaat aaaggtctta
     3901 tcgctcaaat acttaaatta tgtcaaggaa gactttgtgt tcctacatta gagcaaatag
     3961 gagctgttga gttatacaaa actcctgttt cttacttcaa agaagttaat gaagagtaca
     4021 aaaaaagaag agatgtatta tacaatgaat taatgaaggt tgaaggtgtt atatgtaaaa
     4081 aaccaactgg tgctttctat atagttgcta aattaccagt tgaaaatgca gaagatttca
     4141 caatttggat gttaaaagaa tttaataaag ataatgaaac agttatggtt tgccctgctg
     4201 aaggattcta tgctactcca ggtttaggac gtgatgaaat aagattagct tacatcttaa
     4261 atgaaaaaga ccttcataga gctgctacat tattaaaaga aggtttagag caatatgtag
     4321 ctttacaaaa ataatttttt aaacttaatt tatataaatt taatgatata aaatgtcccc
     4381 ttttcagata ttatttgaaa aggggacatt tcttaattaa tatattaatt aagacttaga
     4441 cctgactatt tttcattttc tttatatagt gttggccagt aatctttatt cactttaatt
     4501 aaagcatcta aaacttgtct tgccttagtt agagaaatta taaaaaagta catcaagaaa
     4561 atttaagatg tactttttag attgtattta gacttataat tatatagtta aatgaataac
     4621 aaagaattaa tcttagtacc attaaataca aaaattttta tattttttct atctaaattc
     4681 ttctttgttt aatatatatt gaagagtatt tattgcttct aaatattttt ttctgtcttc
     4741 ttcagttaat ttagaaagtt tttctcctaa accatcactt aatttatcca taaaatcagt
     4801 tgctatctta tctccctctt cagtaatgtc aattagatat tttcttctat caactaaatc
     4861 tcttttcctt gttacatact tcttatcagt taaatcatca accatactag ttaaactgcc
     4921 tttttctata tttaacttag agcataaatc agtcatactt attactccat aattttttat
     4981 gaaaactaat gctcttaatt gtgttttatt tacattataa tctggtgcaa atttttttag
     5041 gtcttctaag tacaggctag aataaatctt aggaaatgtt tttactaaaa aacttatggt
     5101 atcttttact atatagttca tataatcacc tcggttgttt taaaatttat taaattatct
     5161 aaataacttt atttcagttt acattaaaaa tattgcaagt acaatag
//
LOCUS       DAYAOX010000237         5174 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.238, whole genome shotgun sequence.
ACCESSION   DAYAOX010000237 DAYAOX010000000
VERSION     DAYAOX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.238"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            77..1330
                     /locus_tag="ACGI57_003264"
     CDS             77..1330
                     /locus_tag="ACGI57_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFG9718316.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            1479..2531
                     /gene="ytvI"
                     /locus_tag="ACGI57_003265"
     CDS             1479..2531
                     /gene="ytvI"
                     /locus_tag="ACGI57_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HFG9718317.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            2621..3847
                     /gene="pepT"
                     /locus_tag="ACGI57_003266"
     CDS             2621..3847
                     /gene="pepT"
                     /locus_tag="ACGI57_003266"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFG9718318.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            4054..5106
                     /locus_tag="ACGI57_003267"
     CDS             4054..5106
                     /locus_tag="ACGI57_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HFG9718319.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         2030 a          515 c          960 g         1669 t
ORIGIN      
        1 tgatataata aaaactaata ttaaagttat ttataattaa tattaatata aaaacatgta
       61 agtaggagtg tggtaagtgt tggaatcgcc caataatttg attcagatta tttttttaat
      121 agtattactt atagggtctg catttttctc agcatctgaa acggctttaa tgtcgttgag
      181 taaaatcaga atcagatata tgcaagatga aggagttaaa ggagctaagt tagtaagttc
      241 attaatagaa aatccaaata agcttttaag ttctatattg gttggaaata acgtcgtaaa
      301 tatagcagca acatccatat caacatcttt gtttatagga ttaatgggag aaaaaggagt
      361 ggcacttgct acagctgtta tgacagtatt agtattaata tttggtgaga ttactccaaa
      421 aacaatagca gcaaataatt cagaaaaggt ttctctatta gtatctaaac ctataaaagc
      481 aattatattt atattaagac caatagtatg gatatttaat ataattacaa atattatatt
      541 taaattattt ggtataacaa ataaaggagc taaatcattc ataactgaag aagagctaaa
      601 aactatggta aatgtaagtc atgaagaagg cgtccttgaa atggaagaga gagaaatcat
      661 aaataatgtt tttgaatttg gagatatgca agctaaaaat gcaatggtac aaagaataga
      721 tatggtagct atagatatgg aagatagtta tgatgagata atacaggtat ttaaaactga
      781 aaaattaagc agaatgcctg tgtatgaaga aactatagat gatattgtag gaatacttaa
      841 tataaaagat attatatttt tatctgatga agaaatagag tcatttgata ttaaaaatta
      901 tatgagagaa ccttttttca cgtatgagtt caaaaagata acacaacttc ttgaagagat
      961 gaaacttgaa aaaagtcaga tggctatagt tgtagatgag tatggtggaa catctggact
     1021 tttaactata gaagatttag tagaagtcat tgttggagat atagaggatg aatatgatga
     1081 agaagaggat gaaatacaag ttataaaaga agatgagtat attgtagatg gaagtactaa
     1141 aattggtgat gttaatgaac ttattggtgt aaacttggaa tctgaggagt ttgattccat
     1201 aggaggtttt ataattggac acctaagcag attgccagaa gaaaatgaag taattgaggt
     1261 tgataatata agattttgta tagaaagtat agaaaaaaat agaattaaaa aaataagaat
     1321 atatacataa tgtaataatg ttcttaaatt tattagaagt gtataaatat aaaagatatc
     1381 ttttatattt atacacttct ttttattttg tattcaaata aaactagtat gaaatggaca
     1441 aaataaaaat ataaattaga agaaaggaga tttttaagat gagcatacta gaaaataatt
     1501 ttttatacaa attaaaaaac aacactatct ttttttgtat atatacattg ttatttattt
     1561 ttatttacaa agcgtttcca tacatagctc cattttttct aggtgctata atagcactta
     1621 tgattaatcc tatatcacaa aagttagaaa ataagtttcg tataaataaa ggtatctcta
     1681 ctcttgttct aagttttctt gcagtggcta tagtcagtac agttactaca ataatagtaa
     1741 taaattcaat gaaagaatta atgggatttt taaataatat atctgctaat cctgaggata
     1801 taagtaatac tattatgtat tttttaaata aaataaatga ctttatgaaa tcatttcaag
     1861 aaatagccaa ttttgatgta gaacaattag taaacaaatt cagtggtgaa gtaatgcaaa
     1921 ttacaaaaaa tcttcttact agtatacttg gattggctac ttctattcca tatattataa
     1981 tatttataat taccttgttt attgctacgt actttatagc aaaagacctt gataagattg
     2041 aaaatagttt ttataatatg tttactgttg atgttagaaa gaaggtaaaa aacgtaaaga
     2101 aagaggcagg gctttcactt gttggatata taagggccta tacaatactt atggctataa
     2161 cattttttgc aatctgggga agttttgctt tatttggatt gaaatacggt ctaatagtag
     2221 ggtttgtagg agctttaatg gatttaattc cattcctagg tataataaca atatatcttc
     2281 cagtaattgt ttattatttc cttataaaaa attattttat agctataagt atgactgtga
     2341 tattttttgt tttgtctttg attagagaaa tacttgaacc taaactagta tcagtaaatg
     2401 ttggactaaa tccattagct accctagcag cgatttttat tggaatccaa gtaaaaggaa
     2461 taattggagt aattttttgt ttgggtttgg tatgtatgca tgatatactt aagaaggtgg
     2521 atatttttta attataataa ttgatagatt ttttaaaaat aaaattttat tcacaggtaa
     2581 ataaaaaaat attaatctta ttaagaaaga gggtatatac gtgaaacaaa aagtagtgga
     2641 aagattttta aaatatgtat catttgatac aacatctaat tctcagtgtg agaactgtcc
     2701 atcttcagaa ggtcaaagag ttttagctaa gtatatagtt gaagagctaa aaacaatggg
     2761 tgttgatgat gtgagtttag atgaaaatag ttatataatg gctactttaa aaggaaatac
     2821 tgatggtgtg gatactatag gattcatatc acatttagat acaattgaag atgttagtgg
     2881 aaaagatatt aaaccaagaa taatagaaaa ttatgatgga aaagacatag ttttaaatga
     2941 agcgttaaat gttattacgt atgtaaagga ttctccagag ttggaagaat ttaaaggtga
     3001 cgatttaata gtcactgatg gaacaactct acttgggtct gatgataaag ctggtatagc
     3061 tgagatagtg actgcaatag agtatctaat aaatcatcca gaaattaagc atggagatat
     3121 aaaaatagga tttacaccag atgaagaaat tggtagaggt gctgatttat ttgatgttga
     3181 aaaatttgga gcaaaatatg catatacttt agatggagga atagttggag aacttgaatg
     3241 tgaaaatttt aatgctgcaa atgcaaccat tactatacat ggtagaaatg ttcatccagg
     3301 ttcagctaaa aataaaatgg taaatgctat acatatagct gctgaaatat cagaaatgtt
     3361 cccagcagat gaaagaccag aaacaacaga aggatatgaa ggattttggc atttaaattc
     3421 tataggtgga aatgtcgaga atgtaagtat ggcttatatt attagagacc attgtaaaga
     3481 aaaatttgaa aatagaaaat ctattatgat agagaatata gaaaaaataa ataagaaata
     3541 cgataataga gtggaattgg atttaaaaga ctcttattac aatatgaaag aaaagattga
     3601 gccagttatg tttatagttg atatagctaa agaagctatg gaggaattag gtataaaacc
     3661 acgtttagtt cctgtaagag gtggtactga tggagcaaga ttatctttta atggattacc
     3721 ttgtccaaat atatttactg gtggattaaa cttccatggg aaaaatgagt gtataccagt
     3781 tagttctatg gagaaagcta ctaaactaat tgtaagaata gctgaaaaat atgctgaaag
     3841 agtttagtta aaaagtattt aaaaattaag catttaaagt tataatagct atatcattct
     3901 ttataagtca agctgattat aagcttgtgt tgtacttaaa atggtatagc tatttttgta
     3961 attattttta taaaagataa ttatttttag tttagggtat caataaaaag tatgaatatc
     4021 aataggtatt aacatttaaa agggggaatt gtattgaaaa ttaaatctaa aaaaataata
     4081 gcatttgcac tttcacttgg gattatgttg ccaagttgtg tatatgcaga cactattagt
     4141 aaaaatcaaa atgcacaggt agatatgaac aattatcttc aaggtagaaa atctccaaag
     4201 gaggaaaaac ttattggtga agatagattt gatacagcga taaaaatatc tcaaagtggt
     4261 tggaataatg gttcagaaag ggtgtttcta gttaactcaa attcattgcc agatgcattg
     4321 gcttcaactc ctttggcaag taaattggat gcaccaatac tgcttacaaa taaaaatagt
     4381 ataccatata atgtaacaga ggaaataaaa aggcttaatc cagcagaagt tattttaatt
     4441 ggaagtgaag gagctatttc atcaagtgta aaagaatctc ttgaagatat ggaagtatta
     4501 gtcagtagaa tcggtggagc agatagagaa gaaacatctt tacttcttac tagacagcta
     4561 gatgatactg gtgatttagg tgtctcaaaa gtggcagtgg ttaatgggta taatggactg
     4621 gcagatgcaa cttcaatatc aagcccagct gcaagtgata atacagctat aatatataca
     4681 ggtaaggata gcataagaag tgaggcaaag aattttatta cacaaaactc aactaagaca
     4741 tacatagtag gtggggagta cagtataagt aaaaaacttg aaggacagtt agtaaactca
     4801 gaaagactag cagggacaga tagaaaagat actaatgcga aagttttaga aaagttttat
     4861 ggaaaatcaa gtaaggttaa taatatgtat tttgcaaaag atggaagtgg tagagaagct
     4921 gatttagttg atgggcttgc tgctggagta ttagcatcaa aaacaaagtc acctgtagta
     4981 cttgcaagtg gaagtttaag tagtgcacag aaaagtttta taaaaaaagt aaaggctgat
     5041 aaatttgtac aagttggtgg aggtaaaaat agtaaaccat atgcagaagc attagcctta
     5101 cagtaatatt gtgtaaatca aaattaatat ataataatag caagtaaacc aagttgcact
     5161 tcaatatata taat
//
LOCUS       DAYAOX010000238         5152 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.239, whole genome shotgun sequence.
ACCESSION   DAYAOX010000238 DAYAOX010000000
VERSION     DAYAOX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.239"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(638..2773)
                     /gene="rnr"
                     /locus_tag="ACGI57_003268"
     CDS             complement(638..2773)
                     /gene="rnr"
                     /locus_tag="ACGI57_003268"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFG9718320.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            3366..3980
                     /locus_tag="ACGI57_003269"
     CDS             3366..3980
                     /locus_tag="ACGI57_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFG9718321.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            3998..4360
                     /locus_tag="ACGI57_003270"
     CDS             3998..4360
                     /locus_tag="ACGI57_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9718322.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            4466..4963
                     /locus_tag="ACGI57_003271"
     CDS             4466..4963
                     /locus_tag="ACGI57_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFG9718323.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT         1680 a          766 c          620 g         2086 t
ORIGIN      
        1 attctaaaaa agaaaagtta tcttaaataa aaaataagtg tatcttaaat tttaaatgat
       61 acacttattt ttatattgaa attatatttt ttaatatcta ttcttaatta ggtattttta
      121 aattatctgt ttacattaaa tatttcttaa ttacttatct ttcagctagc tttttctatt
      181 aaatgtttct caattactta tttttcaatt agccacttat attaagcatt tcttaattac
      241 ttatttttca attagccact tatattaaac atttcttaat tatttatttt tcagttggct
      301 ttttctatta aatgtttctt aattacttat ctttcagtta gctctttcta ttaaacattt
      361 cttaattatt tatttttcag ttggcttttt ctattaaatg tttcttaatt acttatcttt
      421 cagttagctc tttctattaa acatttctca attacttatt tttcaattag ctactttcat
      481 taaatatttc ttaattattt atctttcagt taactatttc cattagatat ttctcaatta
      541 cctattttta tcatttattt tatagagtat tgtgcactat atatttatac atatcataaa
      601 tactacaact tgttatctct tattttagct gttttatcta ctcctctatt ttttcaacta
      661 tctcaaagtc aatttcttta aaatctacat taacactttc aacctttatc ttaacaaggt
      721 ctcctatctt atatgacttc ttagttcttt ctcctaaaac actataagaa ctctcatcat
      781 aaatatagta atcatcattc atatttgcaa gtcttatcaa tccttctatg gtattatcca
      841 actcaacaaa tattccaaat gaagttacgc tagatacaac accctcaaat tcttgaccaa
      901 ctttatcctt catataaaga gccttataga aatcatgtac atctctttca gctaactctg
      961 cctttctttc tttctcggat gattgaacag atgcataatc cactatagta gtaagctgtt
     1021 cttgtctttt tccacttatt ttattattta aagattcttt tattattcta tgaatttgaa
     1081 ggtcaggata tcttcttatt ggtgaagtaa agtgacagta gaatttagct gctaatccaa
     1141 aatgtccact acattctggt gagtacttag cttgttttaa agaacgaagc attatagtac
     1201 tgatagctcc ttcttctttt ttgcccttta ttttctctat tataccttgt aatgcttttg
     1261 gatgtacttc ctctttatca ccttttatta tatatccaaa tgtagatata aacttactca
     1321 attcttccat tttttccaaa gatggtgtct catgtattct atacacaaac ggtattccca
     1381 tccagtaaaa atgttcggca attgtctcat tacttataag cataaattct tctattatct
     1441 tattagatat tcttctttca tatggcttta tatctacaac ttcgcctttt ccatttagaa
     1501 ttatctttgc ctctgggaaa ttgaagtcta tagctcctct tttttccctt cttgacataa
     1561 gaatcctagc taaaatttca gcatttttaa aatcttcaac aacatgttca aaagtctttt
     1621 taagtttttc atcatccttt tctaatatat cagatacttc tgtataagtc attctagctt
     1681 ttgaatttat tacagattca catatttcat gtttaaccac atttccttta tggtctattt
     1741 ccataaatat agaaagagtt aatttatctt caaatggatt taagctacat actccatttg
     1801 aaagagtttt tggcaacatt ggaattactt tatctactaa ataaactgat gtagctcttt
     1861 ttaaggcttc cttgtcaagt ttatttttct ctcttacata atgtgtaaca tcagctatgt
     1921 gaactcctag tttgaagttt ccattaggta atacttctat tgatatggca tcatctaagt
     1981 cttttgcgtc atctccatct atagtaaata tgtttaaatc tcttaaatct cttcttcttt
     2041 ttatctcttc ttgaggaatc tcaacagcta cagcttctgc ttcttgcaat actttctttg
     2101 gaaattcttc tggaagacca tgttctctta ttatagaatc tatttctacg cctctttcac
     2161 ctttttgacc tagtatctct ataattttgc cttctggttt tctatcctct tgtggccata
     2221 ctgtaatttc acatactacc ttatcattat ctactgctcc actaaaatgt tttttaggta
     2281 tatatatatc tttagtaaat ttcttgttgt ctggtgttac aaatccaaaa gttttacttg
     2341 attggaaagt acctactatt ctagttattt ctctctttat tacttttact attcttcctt
     2401 ctgctctctt ttcttcagtt gcaggtacta ctatttctgc cataactcta tcattatgca
     2461 tagcaccatt tatattgtca cttgagataa ataaatcctg tgtatattca acatcagatt
     2521 ctacaaatcc aaaacccttt ttgtgtgata caaattttcc aacaaagtat cccatttgat
     2581 tgggagatac tatttttcct tttttagtaa agcagatata accatcttct tctaattcat
     2641 ttaaaaaatt ataaaacatt ggcatttctg atgaatgtat atcaaatatt tctgctaatt
     2701 cttctttttt tagaggatta tatgcacttt catttattaa tcctagcaaa ctttctttaa
     2761 gtcctggtat catttttccc tcctgtttta cttttcatta tattattatt tatattgtct
     2821 tttatatttc ttcagacaaa acatttttta attatcacat aattttctat attttcttat
     2881 tattataagt taaaattact tatttatatt tttgatttaa tgtaagcttt gtcatactaa
     2941 cttcaaacaa attatgatgt tttataaatt atttgtaaat atcattataa ttacttaagt
     3001 tattctttat cataattgct taaattactc tttatcacct aattatacac catcaaatag
     3061 gattggtaaa atattcatct catataagta ttactatata ataatttatt ctattagtat
     3121 actcaaatca tgtaaaattt atataactca attaattttt taaatacaac tttataatat
     3181 aaaacttata ataaaagtaa atttatatgt taaatgaggt tgatttttta ttatttttta
     3241 tttatgctag tactaaagac ttgcaaacgt tttttgacaa gtctttattt tgtctaaatt
     3301 aaacaaactt ttttacaaat aaatacataa aatttaaact aatctattta ttggaggaga
     3361 taaaattgga agaagtaatt gcaaaaatta ttactataga aaatggattc aaagaaattg
     3421 aaaacattgc taaaaaaatt gtaaaaaata atgactcaaa aaagtgttat tatctatcaa
     3481 tctacttata taggtcagaa tattatcaag taagagaatt ggctgtgttt atacttggtt
     3541 ttatatcagt aactatttct gaagctttgt tatttttaaa agataatgtg agtaaagatg
     3601 aaaactggag agtacaagaa atactagcta aatcatttga ttattattgc agtgaagtag
     3661 gatatgaaaa ggctcttcct gttataaaag aatggcttaa ttctgatagt ccaaacataa
     3721 aaagggctgt gacagaaggt cttcgaattt ggactagtag agactatttt aaatctaatc
     3781 ctgacgttgc tatatcacta ctttctagtt taaaagaaga tgatagtgaa tacttgagaa
     3841 agtctgtagg taatgctctt agagatataa gtaaaaaata tccagattta gtaaaaaaag
     3901 aattagattc atgggatata tcaaataaaa aagttgagtt tgtatataag cttgcttcaa
     3961 aatgtatctt ggcaaagtaa aatggaggtg aacttatttg aaacttcgtg atgagtatac
     4021 ttgcccatta gaactaacac atgatatgat aaaaggcaaa tggaaatcta ttatattatg
     4081 gagattacgc ataggaccaa cttccttatc aaaacttgaa aaggaaatca atggtattaa
     4141 tcaaaagatg ttacttgaac aacttaaaga attaattgaa tttggtcttg tagataaaaa
     4201 gacatatgaa ggttacccac ttaaagttga atactcactt acacctattc gagggaaaca
     4261 aatattggat gctttggcta taatgcaaga tataggaatt agctttatgt tggataatgg
     4321 tatggaagat attcttaagg aaaaaggtgt tttaaaataa atagataccc acaaaaaagt
     4381 aagtaattta ccattatttt caactggtat ataattaaat tatagatata ttctattaat
     4441 ttattataat aggaggtaaa ttattatgaa agtaactagt acaggtatag taaatggtgt
     4501 tatagaagat aaatacggta aaagaggaac tcagttcaat gaaggtggta tgcctacata
     4561 ttcactacct ctaaagtttg aagatgctcc aaagaataca gtatcctttg ctgtattttt
     4621 agaagataaa gactctgttc ctgtatgtgg gtttgcatgg atacattggt tagctgtaaa
     4681 tataacaaaa gatgagttaa atgaaaatga aagtgtaaca gctactgatt ttgtacaagg
     4741 gactactagt tggcatggca aactaggagg tcttgacaga ttagaggcca gtgtatatgg
     4801 tggaatggct cctccagaca gacctcatgt ctatgaaata catgtatttg cacttgatac
     4861 tatccttgac cttgaaaaag gattctatat gaatgaattt ttcaaagcta ttgaagggca
     4921 tgttttagat acatttacac taaaaggaag ttattctaat taattaggtt gactatagct
     4981 aatcattaat taaatgctac atttaattga tgattagctt aattgttatt agaatatttt
     5041 ctgcacttta tcttaatata tattttagtt gactggtatt ttaatttcta ttagattatt
     5101 atcaatttat ataaattgat tttagcttaa aacttactta tctatttcag tt
//
LOCUS       DAYAOX010000239         5096 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.240, whole genome shotgun sequence.
ACCESSION   DAYAOX010000239 DAYAOX010000000
VERSION     DAYAOX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.240"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..235
                     /locus_tag="ACGI57_003272"
     CDS             <1..235
                     /locus_tag="ACGI57_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFG9718324.1"
                     /translation="EYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDF
                     TYNKMLQKIIESNRVDLEKKNIIRSMKRKTRRC"
     gene            249..923
                     /locus_tag="ACGI57_003273"
     CDS             249..923
                     /locus_tag="ACGI57_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFG9718325.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            949..1785
                     /locus_tag="ACGI57_003274"
     CDS             949..1785
                     /locus_tag="ACGI57_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718326.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            1801..2472
                     /locus_tag="ACGI57_003275"
     CDS             1801..2472
                     /locus_tag="ACGI57_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9718327.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            2462..3694
                     /locus_tag="ACGI57_003276"
     CDS             2462..3694
                     /locus_tag="ACGI57_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9718328.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            3844..4161
                     /gene="trxA"
                     /locus_tag="ACGI57_003277"
     CDS             3844..4161
                     /gene="trxA"
                     /locus_tag="ACGI57_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFG9718329.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            4178..5056
                     /locus_tag="ACGI57_003278"
     CDS             4178..5056
                     /locus_tag="ACGI57_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFG9718330.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
BASE COUNT         2193 a          455 c          892 g         1556 t
ORIGIN      
        1 tgaatactgt aataatatac aaagtgaaga atattatgtt aaaatgggta tggcatggtt
       61 gctatcaatt tgttttatta aatgtgaaga ggaaactttt ctctatataa aaaataataa
      121 tttagatgat ttcacttata ataaaatgct acaaaaaata attgaatcaa atagagtaga
      181 tttagaaaag aagaatataa taagaagtat gaaaagaaag acaagaagat gttgacggag
      241 gaatattaat gaataacaat gaagacagaa ttgacaaatt tttagaactt caaagcaaag
      301 gatttaaatt tgcagagata gctcaaaatc taaagattag tcaaagtact ttaagaactt
      361 ttttaaataa aaaaggatat aagtcagaaa aaggtaaata catattaaaa gacgaattaa
      421 attataataa aagtttggat aatagaaact tggaagaaga gaaagctaag gagtatgaaa
      481 aaagtaaaaa gaatactata agtaatagaa aaaaagtaag caccaaaaaa aagatttcta
      541 atgaaaaggc tttaagtaaa agtgttgaac aaattgagtt taatgataca ggtttaaaaa
      601 atgcaaagcc aaaacaaaat attaagccta agaaagatag aaaaataaat ataactcaag
      661 aagatttaga taagctttgt gaagtatatg attggtattt agaagtaaaa gattacaata
      721 taaagcctaa aaaagtatct aataaaaaag atgtacatat agatgatgaa cacatagatg
      781 aattaaaaac aacaagaata cgtatagata aagaaatatg gaatgatttt gaaagattat
      841 gtagtaattc gaattttgaa aaacatcaaa tacttacaca ggcattgaaa gaatttatga
      901 aaaagtataa aaatctacta taaaatacta ctatatgagg tgcttaatat gaataaaata
      961 gtaaaaaaat caatttctct aatgattata ttaacaatat ttatttttat gttgacagca
     1021 tgtgaaaaag atgaacagcc tgattcagtt gatattcaaa cagaagataa aaatgagata
     1081 aaaatagatg agggaaatgc aaaagtattg aatataggaa aatcagaaat tataaatata
     1141 gatgaaggtg ataaagtaga tacatcaaca aaggtagaaa ataattcaac atttaatata
     1201 tccaatgttg aattgatata taatgaatat gatgagaata aaaaaattat atcttcggat
     1261 agtaaggctt tgttggacat gactttaatg cctgggaaag ttgcttacat agaatgtggg
     1321 cataaaactt ttgtaaaggg tgtagaagtt tatgcatatg aatatgaggc agagggtaag
     1381 attgtgtatg ttaatttaaa agaaaatact attaatataa gaaataataa tattaagcta
     1441 gaaaatagta gtcagtatga ggtgctatct acatcagaac ttaaaaaagt aaatgaatca
     1501 aatgagggaa ttacgtatca agtaaaagta aaaaattcat catcaaaaga tttaggaaat
     1561 attattctta agacagcaga agttaatgaa aatggagaat atttaacagt aaatcgtgta
     1621 ccatcatata agattttaaa gccttcagaa gagacagata tagatatatt gtgttcaaca
     1681 aaggcaaaaa gtgttgaaat agtgggatat acgtatgatg atataaaaga aaaagccaat
     1741 gtggatattg acttaaaatc tcataaagtt aagatagaca aataaaggta ggggatactt
     1801 atgaaggttt taatagttga agataataag atacttttag aaagtgtagt agaagaactt
     1861 agcaagcatt ttgaaactga aaagtgtgaa gatggagaag aagctttata cttaataaat
     1921 caaaatatat atgatttagt cattttagac ttaatgcttc ctaatataaa tggatttgat
     1981 atcctaaaga aaatgagaat taataatata gatacacctg tacttatatt gacagcaaaa
     2041 gaaacattag atgacaaagt agaagcattt actataggag ctaatgatta tttgacaaaa
     2101 cctttttata tggaagagtt agtagctagg gtatacgcta tattaaggac taatggaaag
     2161 ataaaagaaa gaaacggatt agaatttaaa tctttatatt tagatacact agaaaaaaga
     2221 gtgtatattg aaaaagaaga aataaaactt caaaacaaac agtttaactt gcttgagtat
     2281 tttgtattaa ataaaggttc aattttatta aaagagcaga tttatgatag aatatgggga
     2341 attgattcag atgcaactat tgaaatagtg gaagtatatg taagtaatct aagaaaaaag
     2401 ctaagtaaat atggatatga taaatatatt aaaactaaac gtaaggtagg atatatattc
     2461 gatgataaat aagaatgtat ttaccagtac taaaaatcat ttaataaaaa tgtatattat
     2521 tgtagtaggt agttttttaa taatcttttc tatatttata tactcatatt ttaggggact
     2581 tacatatagt ggaattgata gtgagataaa tgatgaactt gaatatatag tctctcaatt
     2641 taaaagaact tcatttttga acccaataag actcaaagat cctaaagata tggtgtatgt
     2701 atatgaagat ggaaggataa gttactatac tcaaaatgaa tattttgatg agttacttcc
     2761 agatagaaga ctagataaaa aaaattcgtt ttttaaatac acagaaaatg gatatacttt
     2821 tagagaatta aatgtagatg taggtagata ccaaatacag ataattagaa atatagattc
     2881 tgagatgaat tctttaagac agcttacatc tgttcttata attggaatat taatttctgt
     2941 aataattact tactttgtgg ctgtatatct cactagaaaa gctttaattc caatagaaac
     3001 agcatggaaa aatcaagcta agtttataca agatgcttct catgaattaa gaacaccaat
     3061 aacaatagtt tcatcaaaat tagaaagtat gttaaaatcc ccagaaagta cagtaaatga
     3121 tgaagttgaa acaatagcta ctgcaatgaa ggaaactaga agacttaaaa aaatgataac
     3181 tgatttattg tctcttacta aagaggactc tattgttaaa gttaatttag aagaaataga
     3241 tttagaaaag ttattggaag aaatatcaga agactatatt gacattgctg agtttcagga
     3301 aaaaagattc gtgtttaatt ctaaactgaa gaataaagtt ataataacag ataaaaataa
     3361 attaagacaa cttatattaa ttttcattga taatgccttt aagtatacaa aattgggtga
     3421 tgagatagca ttagaattga aagaagatat agaggatgag gtaactttac ttataagtga
     3481 tactggtata ggcataaaaa aagaagagat acctcttata tttgatagat tttttagaag
     3541 tgaaaatgta agaaataaag atttagaagg ttctggaata ggactttcaa tagctcgcat
     3601 gattagttta aacttatcta ttgatataaa tgtaacttct gatgtagata ttggaactac
     3661 atttgaattg tcaataccaa aaaagttaaa ataataaaaa tataaaaaat tgtttttaga
     3721 aatgaagaat gggtatacta attagtataa aatataaaaa gaccttataa aagtaaattt
     3781 atatttataa atttcatagt ataattatat agggataata aagtttaata ggaggattta
     3841 aaaatggcaa aagttataaa tacatctgaa tttagaagta gtgttgaagg tagtaaggga
     3901 gtagttgtag ttgatttctt tgcaacttgg tgtggacctt gtaacatgtt gggacctgta
     3961 tttgcagaat taggtgaaga gatgaaggat aaagctagat ttgtaaaagt tgatatagat
     4021 gagagtttag aaatagctca acaattcaat gtatctacag taccaactat gattatattt
     4081 aaagatggaa aaccagtaga aactttaatt ggattcatgc caaagaacaa aattgaaatg
     4141 caagtaaaat catatttata aaaaatgggt gagtattatg agatatgata tagctataat
     4201 cggcagtggt ccagctggat tgtctgctgc tataaatgca aaaataagaa ataaaacaat
     4261 tatcatgttt ggtaatgata atttaagtaa taagcttgtt aaagctcctt caatagataa
     4321 ttatttaggt ttttatgata taagtggtga tgaattaaaa gacaagttta aatcacatat
     4381 agatagtatg gatatttcaa ttgaaaataa aagaataaat aatatttatg ctatgggaga
     4441 atactttaca ataatgtctg gaaatgatat gtatgaagct actactgtaa tccttgcaac
     4501 tggggtagag tatactagac caatcaaggg tgaagaagaa tttctaggaa gaggtgtggg
     4561 ctactgtgct acatgtgatg caccacttta tagaaataaa aaggtagcag ttataggata
     4621 taatgaagaa tctaaagaag aagcaaattt tttaagtgaa ctcacttcaa agacttattt
     4681 tatacctatg tacaaaaaag ataatcttat gagaagttca gataacctag atgattctat
     4741 tgaagttata catgacagac cagtacagat agatggagat aaacttgtaa acaaagtatc
     4801 ttttaaagaa aaccacattg aagttgatgg agtattcgtt ataaaagata gtacggctcc
     4861 aagtgcattg gttcctggaa tagagattga tggcattcat attaaagttg acaataatat
     4921 gaagactagt atagatggat gctttgctgc tggagattgt gttggaaaac cctattctta
     4981 tataaaagca gctggtcaag ggcaaatagc agctttaaat gctgtttact atttagataa
     5041 attaaagagg gcttaatatt tatattgatt aactttttaa agatatttat aaaaaa
//
LOCUS       DAYAOX010000240         5040 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.241, whole genome shotgun sequence.
ACCESSION   DAYAOX010000240 DAYAOX010000000
VERSION     DAYAOX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.241"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            634..864
                     /locus_tag="ACGI57_003279"
     CDS             634..864
                     /locus_tag="ACGI57_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9718331.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            857..1438
                     /locus_tag="ACGI57_003280"
     CDS             857..1438
                     /locus_tag="ACGI57_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9718332.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(2017..2694)
                     /locus_tag="ACGI57_003281"
     CDS             complement(2017..2694)
                     /locus_tag="ACGI57_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9718333.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(2883..4247)
                     /locus_tag="ACGI57_003282"
     CDS             complement(2883..4247)
                     /locus_tag="ACGI57_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9718334.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            4375..4896
                     /locus_tag="ACGI57_003283"
     CDS             4375..4896
                     /locus_tag="ACGI57_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFG9718335.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         1909 a          662 c          804 g         1665 t
ORIGIN      
        1 taaagataaa cttcaatatg ggtttaactt gtggttaata gagtaagaat gtaaatagag
       61 ataaacttca atatgagctt aacttgtgat taatagagta agagtgtaaa tagagataaa
      121 cttcaatatg ggattaactt gtgattaata gagtaagagt gtaactaaag ataaacttca
      181 atatgagctt aacctatgat taatagagta agaatgtaaa tagagataaa cttcaatatg
      241 ggtttaacct gtggttaata gagtagggtg taactaaaga taaacttcaa tatgagttta
      301 acttgtggct aatagaataa gattgtgaat aaagttatag ttttagatgg gtattaacct
      361 gttactaata gaatatgatt aggtgtaaaa ttatagagct gcataagaaa atattataga
      421 tatattaaat attagataat aaatatttat atagatacac aaaagaaaag gcgatatgaa
      481 gctaaactta atgacttcat atcgtttttt atttgaaact cctttgtatt tagagtgtga
      541 tttttaatat tgaggtttta tgtgattaag tcacagataa aaagtaagta tcttgatata
      601 attcttatag attaataata tagggaggtt attatgtcta ataaaaaaaa gaagtatgca
      661 aatattttag caaatgagtg cgttgcttgt ggtagctgta taaaagcatg tccaagaagt
      721 gctatctcag ttccatgtgg cattagtgca aaaattaata gagatttatg tgttggatgt
      781 gggatttgtg aaaagatatg tccagcatct gtaattgaga ttataacaat attaaaagag
      841 gaagaaggta agtgtcatga ataataaaaa acattggtat gactatttat ggattttttc
      901 ttcaatatat ttgatattgg gatttgtaaa tattattttc gcttggattg ggctaatttg
      961 tttctttgtt ccactagcta tttctattgt taagggaaat aaagcttact gtaataagta
     1021 ttgtggaaga gggcaactgt ttaatttgct tggaaataaa ttgaagctgt caagaaatag
     1081 agatattcct aaatttatta gaagtaagtg gtttagatat gggtttttga cattttttat
     1141 ggtaatgttt gccaatatgc ttttttcaac ttatttagtg tttgaaggaa gtagaaattt
     1201 taaagaagta gtgactttgt tgtggactat aaaaatgcct tggcaatggg cgtatcatgg
     1261 aactttagtt tcaccttggg tggtacagtt tgcatttggt ttttatagtg ttatgcttac
     1321 atcaactgtt cttggtttga ttactatgat attgtttaaa cctcgttcat ggtgtgttta
     1381 ttgccctatg ggtactatga ctcagatgat atgtaaggca aagagtaagt ctgtatagaa
     1441 aggccttatg tgatatttac agttttgttc aagtagagat gagtagtagg tttgaatata
     1501 tatgacaaat atatgagtat aggggcatag atttagtttg tgtaaggtaa actgatttat
     1561 gcttcttttt tatgtcttag attattggct aagtggaaac aatgaattta gatattaaga
     1621 attattcagt tatttgaaga aaatagaata ataattaaca ttatattaat ggatattcat
     1681 tacatataag ccagttaata gcaagattta tttaatatga tataggtcaa gtatttaaac
     1741 taaataaggt catgacaatt aatataagta tagatataaa aagattagaa gttataactt
     1801 ataaaaggta ttctatataa atagtatggg gaaaatagtc aaattaactt attagaaaca
     1861 aaaaatatac ataaaaaata aatgtaaata tcaaataaga tactatatca aaatagatat
     1921 aagtatcttt tttattattg aaattatgaa aatatatttg attttacaca aaaaagagtg
     1981 cctcatgaac taaaaagttc attttgagac acacttttac agtgtcaaag aacgaagttt
     2041 atccttatcc aataccttta taccaccacg agataaagac acaatacctt ctctagcaaa
     2101 atatttcaac attctcgata caacttctct ggcactaccc atatacttag caatttgctc
     2161 atgagtcata tttatagtgc tactgccatt tttagctatc tcatcaatta aaaaagatgc
     2221 caaacgctta tcaaaactca taaaaagaat ttgttgcata gcccacataa catcagaaaa
     2281 acgttcagta gctaatttat atgaaaataa ctctgcatga atattttctg tagataattt
     2341 tgcaaaaaca gaggaactaa tctgtataat atcgcagtcc gtttcagcat ctacatagac
     2401 atcaaaagtt attgttttta gaatacagga agctgataaa atacatacat ctccatcatc
     2461 aagtcggaac agtgtaacct ctcgaccttc atctgaaagt atgtatgtac gcactgttcc
     2521 agattttata atcaaaatac caatacagtc actatcacca caatgtactg gattcccttt
     2581 tttgtaatgc gaggtatttg ctgaagaaat taataattct ttttgtaagt cagtaagttt
     2641 atcccaaaaa ggcaagctat gtgtaagtat atcaatttca ttttgtgaaa tcatacaata
     2701 tctcctttct taaattgtta taatcccatg atacaagtga atagataaat tgacaagtag
     2761 tttgttaaga ggattattta gtcagatagt aatagtatga tttatgtaga aaaaatatga
     2821 taatatatat tcttgctatt aatgagaaag taatatgttt tgatgatagt aggtttgtat
     2881 aactaatgca taccgtttat ttttaaaaga ttaagttcct tatgtaacct tatagacata
     2941 aaaccagcaa ttatagcagc catcaaatca gctattggtt gtgcatataa gataccattt
     3001 aacccataga atacaggaat aatcaaaatt atagggataa aacagacacc ttgtctacac
     3061 atacctaaaa atccaccttc ctttgctttt cctagtgcga ggaataatgt agaatacact
     3121 gtatagaatc caaatagcat aaatgaaaac ccatttgccc taagagcttt actaccattt
     3181 tctattaata aaatgtcacc acttgtaaat tgtgaaatta tagattttgg gaagagtatc
     3241 aatataagac caagcactac acaaaatact gtagtccata atatggaagt ttttattgct
     3301 atatgtaatc tatcgtagtt ctttgaacca aaattataac cagcaattgg ttggaaacct
     3361 tttataaaac caaatacaac aagactacct aaagacgtta ttcgtgttac agcacccatt
     3421 ccagctatca cagaatcacc atactttgta gcctccatat tagttaatgt aatagcaaga
     3481 ctagtcaata attgaaatat taaggtaggt atgccaattt ttaggatttc tgaaaaaatt
     3541 tcttttgaaa aacagcattc gttaattcta aaactaaata tacttttctt gcttaaaata
     3601 tatcctacat aaacaagtgt tgaagcaatc tgtgagatag ctgttgcaat agaagcaccc
     3661 cttaccccca gaccaaaagt ataaatgaat atcgggtcta aaatcatatt aagcaaagca
     3721 cctaataaca tagcacacat agttgtttta gcagctcctt cacttgtgac aatattagtc
     3781 atagtgacat tgaatatatt aaaaatagat gacacgacat aaatgctagc ataagatatt
     3841 gcataaggca taatactttt tgtagctcct aacaatttta aaattggatt gagaaaaatc
     3901 ataatcacaa taataactaa tgtacccact ataatactac tgtaaataga tgtacttacc
     3961 acttttcctg ctttttcatt atcaccatgg cctaaaagtc tggcaacata tgaagcagct
     4021 ccatttccaa acaataatcc taatcccaca acaagctgac ctaatggaaa tgcaacggat
     4081 atagctccca tttgacttgt tccaagtcca cccacaaagt atgcatcaac caaattgtat
     4141 aatgcagtta tcatcatacc tatcatggta ggtaatccca tagataacaa tgcttttgag
     4201 attggggcag accctaataa ttcaattctt ttgttacttt ctttcatact taacactcct
     4261 cttctattga cattaagtct aaattataat actataaatt atagtaagta tggaaaatag
     4321 tactataatt tatagtattt gtcaatagta agaaaactaa aggagatgat ttttatggct
     4381 acaatagatt taattgtatt gggaatagta aagcaacaac ccaagagtgc ttatgatatt
     4441 caaaaggcag tggaatacag gaatatatca cgttgggtta aaataagtac accatcaatt
     4501 tataaaaaag taattcaatt agaacaaaag ggatatattg aaagtaaaac tgtgaagaat
     4561 ggaaaaatgc cagaaaaagc agtttattca ttaactgaat taggtgagac acatttttta
     4621 gatttaatgt ttgatacatc tagcaaacca ataaatattc ttttagattt taatgctgtt
     4681 atagtaaatt taaatagcct tacaccagaa aaacagaaat tatgtctcaa taatattgaa
     4741 gagaatgttc aagagttaaa gaaaactatt gaggaaaata taagtataaa gacagaagta
     4801 ccagacacag caaaagcagt gttggagcaa cagttggtgc tagcacaagc attggaaacc
     4861 tggataaatt ctttaaaaga taatatggga aattagaatt agagaagcta acttaataat
     4921 cattaaacaa aaaccagcat atttaataat gagatattag aaaaaataaa acaaatgata
     4981 taaactgaaa agtatattta aaataaagct cacttaattg agggagaatg ttaaactttt
//
LOCUS       DAYAOX010000241         5027 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.243, whole genome shotgun sequence.
ACCESSION   DAYAOX010000241 DAYAOX010000000
VERSION     DAYAOX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.243"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            386..1558
                     /locus_tag="ACGI57_003284"
     CDS             386..1558
                     /locus_tag="ACGI57_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFG9718336.1"
                     /translation="MIIWLSDPINILFTLFGYFILLIICSKSKLIPIISVVMILYPII
                     IGLNFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQY
                     LSNKIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAIL
                     ANIGVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIES
                     YLEIEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNID
                     IDNHLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKV
                     NPINRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(1894..2463)
                     /locus_tag="ACGI57_003285"
     CDS             complement(1894..2463)
                     /locus_tag="ACGI57_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HFG9718337.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVEGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            2866..3438
                     /locus_tag="ACGI57_003286"
     CDS             2866..3438
                     /locus_tag="ACGI57_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HFG9718338.1"
                     /translation="MEYIINPCSGIKIKITRGQKIEIVDIEGGQVVDFFAEMKDDSTE
                     FISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPCCRPEMYDFFYH
                     NGENHPNCFDNINRTLGTSHQIIHPINLFMYTKVNTDGSIEVCEPLSKANDKIILEAL
                     ENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(4117..>5027)
                     /locus_tag="ACGI57_003287"
     CDS             complement(4117..>5027)
                     /locus_tag="ACGI57_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFG9718339.1"
                     /translation="GNVLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIG
                     TFGKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSF
                     GSGAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPL
                     TGIILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKER
                     GEKILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVT
                     MTDERDSGAVYDKMENLCKEHTAHIF"
BASE COUNT         1814 a          818 c          583 g         1812 t
ORIGIN      
        1 ttttgaagct tagtttttag agtttttttc aaatttaatt ttttgaaact taatttttaa
       61 aatttcaatc ttttaaaact caattttttg aagcttaatt tctagaattt tatttttcaa
      121 aatcaattct ttgaaatcta atttccagaa ttttaatttt taagatttaa ttttttaaaa
      181 cttagtttcc agaatcttaa ttaatagaaa ggtatattaa atgggcactt attctttaat
      241 ctctacaagc ataaatttta ttctaatatt aatcaatatt ttcatagcct tttttcttta
      301 taagttgact tcctatttta tagactttag gaatcattgg attttcaaaa tcatattgat
      361 tttgggctac tctattatta gtcaaatgat aatatggctt tccgacccca taaatatttt
      421 gtttacacta ttcggatatt ttatactact tataatatgt agcaaaagca aattaattcc
      481 tataatatca gttgtcatga tactttatcc tattataatt gggcttaatt ttctttttat
      541 aaacaaccct atatatgagg aacttataca ctcaaaagag tatattttaa tactttcaat
      601 agtccagata atttctgctc taattaaatt attattttgg tttttaatac atattttcct
      661 aaagaataga ttggtcaata taaaacaata cttatcaaat aaaatatgga tttttattga
      721 tatggtatgt atatcttcat ttttatcaat attaattgct attattctgc cacctactaa
      781 tttaatccct atgggtagta ataagaatat tatcttaggc aacttacctt atggcactta
      841 tctaatcatc ttgtcagcaa ttctggcaaa tatcggtgtg atacttcttt tacagccatt
      901 gattgaaaat gtaaagatga agatagaaaa acaagcaaat cacctaaaag aaaaatacta
      961 taacttacta gaaaatcaac aaattcaaat acgcaaaatg cgacatgata tgaataatca
     1021 ctttcaaatg attgaatcgt atttagaaat tgaagatatt gctggagcta aaaagtattt
     1081 taatcagtta aaaataggta taggtactct tagtggaaaa caattttgca aaaaccaagc
     1141 tcttaactca attttaaatc ttcgtcatac aagtttagaa gaaaatggtg ttgatgtgca
     1201 ctttaatata gatatagaca atcacttggg tatagaacct atagatttat gtactatttt
     1261 ttcaaattct ttagataacg ctatagaagc atctttgaaa attcctgatt tgtcaaatag
     1321 aaaagtggta ttaaaggcac gttgtgagaa ggagtatttt agctatttat tgacaaacaa
     1381 caaggtaaat cctatcaata gaaaacaaga actatttatt tcagataaaa aagatagtaa
     1441 ttctcacgga tatggtattg agaatattag agaaattgtt aataagtaca atggaaaatt
     1501 ggatatttct tatactgaga gtgaattttc tttatttctt tatattagat tattttagtc
     1561 taaaattaga ttatcttata taatacaaat tagattgttt gatactatgt aaaattataa
     1621 taacttataa ttaaatattt acacatatat tcattaactt aaaatttaat caaatgtgat
     1681 attttattta ccttagtaaa aatgtgtgtg tctcaaaata aactttttag ttgatgaggc
     1741 actctttttt acatgaaatc aaatatattt ttatcctttc aacaatgaaa aatggattta
     1801 tctctccatt ttgaaacaag ccctttttct attaattact ttcttaaaaa atataatacg
     1861 atatataaga taaatatcta attgtaccta tttctaatta tatctattag aatacaacac
     1921 ctttgccttt tcagtaaact ccctaacaaa atctagttta ctatctgtat aagcatcacg
     1981 attatgctca tacttcttcc aaaggtccaa cttaagtttt tcatactcct ttgcaacatc
     2041 tgggtggtca attaagtaat ctctaaaaaa caactcatca ttatcaccta cataccttat
     2101 atgcaaatga aaaactcgtt cttcaaaccc ctctactgta taccctttat taagtgaaag
     2161 cctactactg ctctcagaca tacaaatata tccattcgac tcaatcaaat ctttaatatc
     2221 tttcatatta taatcaggaa ttatctcaat cagtatgtca acaatgggct tggcataaat
     2281 cgtactcaca gaagtacttc caatatgact tattctaaca attttttgca ttggcaatat
     2341 tccagttagt ctatattgtt cttctttaaa ccaatttttc caacattctt tgtgagtagt
     2401 caaaaatata ggaaacaagt tccataactc ctctaaagtc atatcttcca acttcttttt
     2461 cattagttac ctccttttaa atttgtactt tatatttttt ctttttcaaa tttacttagt
     2521 taatatttaa ctttattttt tatcaaatca tttttctaat tatgtaaatt tttctaaatt
     2581 tagtttttaa tgtattataa cagattttta cccttcttac acttttgtat ctatttaaat
     2641 attagaacat tttttcttac ttctaaatac agtaagattg cttaaatacc aaataggagc
     2701 aaaaaaactc tgtaatctaa aaaaaactac aacatcttta tgagaattaa aaaatgatag
     2761 actatgttat caacattaag aaacagatag aaaagaaaca gagtatgtgc taaaatggga
     2821 acatagaaca gaatgacaaa ttggaattta tggaggacat taaatatgga gtatataatc
     2881 aatccttgta gtggtataaa aattaaaata actagagggc agaaaattga aattgtggat
     2941 attgaaggag gacaggtcgt tgattttttt gcagaaatga aagatgattc aactgaattt
     3001 atatctccag gtgttacaat tgattgtaat gagtcattaa gattacaagt tgggaattta
     3061 atatattcta ataagtatag acctatgttt aaaattttat atgatgatgt aagggaacat
     3121 gacttgctac atccatgttg tagacctgag atgtacgatt tcttttatca caatggagaa
     3181 aatcatccaa actgttttga taatattaat cgcacacttg gaacttccca tcaaattatt
     3241 caccccatta atttgtttat gtacacaaaa gtcaatacag atgggagtat tgaagtatgt
     3301 gaacctttat caaaagccaa tgacaaaatt attcttgaag cattggaaaa catgacttta
     3361 gggattgctg catgcagtgt ttcagaaagt tcttgtaata gcagaaaatg tacttctatt
     3421 aagattttgg ttgattgaca aatttcattt atcgaaaaaa tatcaaaatt aaaaaatgac
     3481 gtcaaaggcg caaaagggta accacttgcg ctctcaattc attatggttt gttacgtatt
     3541 tatgggtaaa aatcccttga ttctatgatg tttttcagac acaattacaa atgagtaagg
     3601 attttatact catactctaa aaaatagcat tttattgcgt aacagaacga aaaaacagtg
     3661 tgtacctttt tcttgcaata cacactgtat tcatagccac tctagctgtt aactgttaat
     3721 tatctcataa tacccttact ggtattcatc taatcttaac cccttttaat tattttcatt
     3781 tataaataaa ttctattctt cccaaaatat cgtaattaaa atatattaat taaaatatcc
     3841 caataagaat atctgaaaaa tcttctacct attttttcaa accttttatt cccattcctc
     3901 atacaatagt gcaactttta gcacagtata aatataccat tagtccatat aatctcatta
     3961 acaaatatat acaattattt tcttcaaact cttttatatc taagatatca cattttcatt
     4021 ttcttatatt aattccttaa ctctctttca aattaccaat tttttaatca aatcttcatt
     4081 aaatattcta tcattattaa acattcatca ttatccctaa aaaatatgtg ccgtatgttc
     4141 tttacacaaa ttttccatct tatcatacac agcaccacta tctctctcat ctgtcatagt
     4201 cacaattaca tcacttggtc taagaattgt cttacctcta ggaataattt cttctccacc
     4261 tctttgaatt gctaccaaaa gacaattttc aggccactgt acatcctgaa ttgccttgtt
     4321 cgcaacagga gagccaaaca taacagagaa ctggtctagt atcttttcgc ccctctcttt
     4381 atgtacaggt tctccattct tttcaagtaa cctctcaaga agactttcat aaataggttt
     4441 agacttcata agtgtagcca caatatatgc cacaattgaa attactgata gtgaaagcat
     4501 ttgactaaca gaacctgtca tttcaaaaat taatatgata ccagtcaaag gggcacgcac
     4561 aatagctgta aaatatcctg ccatagccaa aagcacaaag ttatttacat aatcgggatt
     4621 cattccaaaa actttaacac ctaccatacc aaatattcca cctataaatg ctcctaaaac
     4681 aagcaatgga aaaaatattc cccctggtgc acctgaccca aaactaacag ccgaaaatat
     4741 aaaacgtata acaaaaatca aaacaaccat tccaaaaata tatttatgac ttgtaagttc
     4801 ttcaactaaa ttgtgtccac tccccagaac atcaggcata acaagcccaa gaataccagc
     4861 cattataaat ggaatcaaca gttttccaaa cgtaccaatt ttaggaatct tcttgtataa
     4921 agactgcaca tatagagtaa accagttgta aaatgccccc ataactccaa gaataacacc
     4981 taaaatcact attaatccat aataatcttg tggcaacaca tttccaa
//
LOCUS       DAYAOX010000242         5005 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.244, whole genome shotgun sequence.
ACCESSION   DAYAOX010000242 DAYAOX010000000
VERSION     DAYAOX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.244"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(239..1957)
                     /locus_tag="ACGI57_003288"
     CDS             complement(239..1957)
                     /locus_tag="ACGI57_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HFG9718340.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(1962..2471)
                     /locus_tag="ACGI57_003289"
     CDS             complement(1962..2471)
                     /locus_tag="ACGI57_003289"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFG9718341.1"
                     /translation="MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKI
                     AILNGGDIMQIGTPEEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKE
                     NYQGIISNIEFRGNLLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYY
                     LKYPKVKGA"
     gene            complement(2481..2831)
                     /locus_tag="ACGI57_003290"
                     /pseudo
     CDS             complement(2481..2831)
                     /locus_tag="ACGI57_003290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     regulatory      3120..3217
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3272..3448
                     /locus_tag="ACGI57_003291"
     CDS             3272..3448
                     /locus_tag="ACGI57_003291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718342.1"
                     /translation="MFRDKMDKCTHVLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     gene            3555..3755
                     /locus_tag="ACGI57_003292"
     CDS             3555..3755
                     /locus_tag="ACGI57_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718343.1"
                     /translation="MCFKTYILKRVVNNKQLEITRSFELDNLICTATEILKDDEIDLI
                     IIDEKNSIVWRNEKSTPQQENA"
     gene            complement(3934..4806)
                     /locus_tag="ACGI57_003293"
     CDS             complement(3934..4806)
                     /locus_tag="ACGI57_003293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718344.1"
                     /translation="MSNINNKNKSWIEKHKILTIFISVIAILIICIGLPSLINRVLYW
                     DIKTHNSTDGEWLSFWGSFLGGIFGGLATLGAILITREMAKKDNEELKESISMEREPK
                     VIPIKKNAYLYKTYGSPCMFNEIKTSSEVPRCSDIIELTLVNVGKEAAIQIEVFWEKP
                     LNIVKTEYLTDDDLEVCNNFFDEFDENENIKVEKEYISSLQEVEIGLNAYTEMYIREL
                     SSKLIDNFRENNEDGIYIRKNIKAGTINIKCKNIYDKSYNKSYDMEISIFEMDRSKEL
                     YTINIDFNLIEDEG"
BASE COUNT         1713 a          754 c          588 g         1950 t
ORIGIN      
        1 agttgaagaa aactcaacta atgaattaaa attatcaatg tctattggat atgctatttt
       61 tgatgaaaaa gtagatgttt ctttatttga tacatatcgt cgtgcagatt ctcaaatgta
      121 tgataacaaa aggaaaatta ataaacaata tcaataataa atttaaaaag tctattcttt
      181 aaataataaa tcacaagaat agactttttt aaatttgtgt ttttataaga taaattattt
      241 aattaaagtt tgttcttttt tctaaacgat tctttataaa ttcatatatt actttcacta
      301 atatatttgt gactaatatt aacacagcta gtgatgctgc ctgtgcagta ctccccttat
      361 catctagttg tacaattgat attgcagcta cttttgaact tgcagtatat ataaaaacta
      421 atgctgatat tgtcaccatg gagtttagga aaaagtacat aaagttctct aaaactgctg
      481 gcaaacaaag tggtatagtt acgttcttaa gaattgatgc gctgtttatt cccattgatt
      541 tagctaccat atccaattct ttatctactt tcttaatagc agtagtagct gttataaatg
      601 ctactgaaaa gaaatgaact atattacata taaccattat aagtaatgtt ccatataatc
      661 cattaaacaa attcttaatg tatattgtat ccataaaatt aaactctaat ttattaaaaa
      721 ataatacata agctatacct aaaactaatc ctggcaatgc cataggcaat gtacttaaaa
      781 aatacccaaa tgattttaaa tatggagctt tttctgtttt ttcattcaaa tatgcaaata
      841 agaaaacaaa gctcgtccct agaatagctg tcaatagtga catttttatt gaatttataa
      901 atggtttaat cccatcaata ctgtttttca tagaaaaatg ttccagtgtc agtcttaaat
      961 catatggcca aactgatatt aatgaagata agattacagt taacattaca gctattatac
     1021 caaatgcaat aattcctaca acccctccta aaatactatc tcttaactta ttagttttaa
     1081 ccttatatgg aattgacttt gaagttatat atgaattttg atttttttca atttttttag
     1141 ttactataaa cgatattatt gcaggtatta aaagtaatat tgatacagtt gctcccattg
     1201 gcatattctg ttgccctata atttgcttat atacatctat tgataataca ttaaactctc
     1261 ctcctatcac ctttggagct ccaaaatctg taaaacataa tgtaaacgat acgaatgcag
     1321 aacttattaa agagtatttt atacttggta ttgttatgct tataaattct cttatcttgc
     1381 ttatccccat agtattacta gcttcatata gtctataatc ggttgaactt agagatatat
     1441 aaagtatttg aaatgattga ggaaatacat atataatttg tgccaatatt attcccactg
     1501 ctccatatat tggaacatgc actccaagta aattagttac caaaccttga tttccaaata
     1561 aatatactag acctattgca tgcatcattg ttggagcaaa cataggaaac atagctaatg
     1621 atttaaaaaa acctttaaat ttaatctttg ttctagttaa tgcatatgca tatactaatg
     1681 caagtgttag tgatattaca gttgatataa ctgatacaaa tactgtattt tttagagaat
     1741 ttaatagaga ttcagaagcc aagtattctt gaaagttttg taatcctaca aagactccat
     1801 ctttatctaa aaaagcctgt acaaatagat atattatagg tgctaataaa aatattacta
     1861 aagacataac taatactgta agcatacatt tttttatatc taaattcatt tttgatttaa
     1921 gacttatact tttagaactt atagcattat tattcataca cttatgctcc tttaactttt
     1981 ggatacttta aatagtattt ttcgtctata cttattttta caaggtcacc ttctactaaa
     2041 tttaaattaa cccactcttt tatagaagca tcacttatta tgaaattttc atttaattta
     2101 tcttttattt caactctcaa taaattccct ctaaactcta tatttgatat tattccttgg
     2161 tagttttctt ttgtcgattt ctctatttga acatattctg gtcttacagt taatatagaa
     2221 tctccattat caaaacagtt tgtatctcct ataaattgag ctgtaaatag gttttgagga
     2281 ttttggtata tttcttctgg agtccctatt tgcattatat ctcctccatt tagaatagct
     2341 attttatctg ccatagttat agcttcttct tggtcatgtg ttaccattat tgtcgttata
     2401 ttaagttctt tttgtaatct tttaatgtcc attctaagct tgtgtctaac ctttgcatct
     2461 aatgcggaca ttggttcatc taaaagtaaa aactttggtt caagagctaa tgctcttgct
     2521 attgcaatcc tctgttgttg cccacctgat aatgctttag gatatttatg agcttcatta
     2581 gtcaatccaa ctgtttcaag tacttcctta actttattat ctatttttcc ttttgaaact
     2641 tttctctctt ttagtggaaa agctatattg ttataagcag tcatattcgg aaataatgca
     2701 tatgattgaa atactatacc aaaacctctc tttgatggct ctaaatttgt aatatcttta
     2761 tcctggagaa ttatagttcc gctatttaca tcttcaagac ctgctattat tctaagtaat
     2821 gtagttttac cacatccact tggctattat tagttagtcc aaataaataa aatacttata
     2881 acaattgaaa tacgcgatat acactgaacc tcccatgact aaagtcacag ggttcctgtt
     2941 tcatagaatt tcgcataatt aaatgttagt tcacaatact attctaactt accttgacct
     3001 ttaaatactc tgttcctttt taggaataaa atactattca tacctataaa tttattcaat
     3061 tttcaaacaa tcttgttgaa caataactaa ttctattgta ttattttagt tgtagaataa
     3121 aactaaatcg gcaaaactag agaaatttag tgacgcaaag ctatagggac taagacttat
     3181 ataaatatct tatgagttat gtcagccagt tgccaaagag atattgttct ttttgttttt
     3241 atgaaagttt tttaggggga taataattta tatgtttaga gataaaatgg ataaatgtac
     3301 acatgtattg actgcttata ttggtagttc atatgattat tgtgatttta tagatacaca
     3361 gttagatgat tttatactag agtacggaga aaaagttgtg gaatcttgct tgcatcaagt
     3421 aatggtattg gtaagtaagt ataattagat agacattgat ttctagagaa ccctttttag
     3481 ttttatctac ttttaaataa tgatatgtta aaaaattaat taaaatatct ttgtaagaaa
     3541 ggataataat ataaatgtgc tttaagactt atatactaaa aagagttgta aataacaaac
     3601 aactagaaat aactcgaagt tttgaattag ataatctcat ttgtactgca acagagattt
     3661 tgaaagatga tgagattgat ttaataatta ttgatgaaaa aaatagtatt gtttggagaa
     3721 atgaaaaaag cactccccag caagagaatg cttagttata gctatagaag tgtactacga
     3781 atacacttct atttttttat tatatcataa attattcata gtattaagta aaaatagagc
     3841 taatacaatc agttttaaaa tagcttgaat agctttcttg agttatttac ctttgcataa
     3901 attattgaat atttgaaata aaaaaagata ggtttacccc tcatcttcaa tcaaattaaa
     3961 gtctatattt atagtatata attctttact tctatccatt tcaaatattg atatttccat
     4021 atcatatgat ttattatagg atttatcata tatattctta catttaatat taattgtacc
     4081 cgccttaata tttttcctaa tatatatacc atcttcatta ttttctctaa aattatctat
     4141 aagtttacta gataattccc taatatacat ttcagtatat gcgtttaaac ctatctcaac
     4201 ttcttgtaaa gaagaaatat attctttctc gactttaata ttttcgtttt catcaaattc
     4261 atcaaagaaa ttattacata cttctaaatc atcatctgtt aaatattcag ttttaactat
     4321 attcagaggt ttttcccaaa atacttctat ttgtattgct gcttctttcc ctacattcac
     4381 aagtgttaat tctataatat ctgaacatct tggcacttcg cttgatgtct taatttcatt
     4441 aaacatacaa ggactaccat aagttttata caaatatgca tttttcttaa taggaataac
     4501 cttaggctct ctttccatag atatactttc ttttaattct tcattatctt ttttagccat
     4561 ttctctagtt atcaatatag cacctaatgt tgctaaccct ccaaatatac cacctaaaaa
     4621 actgccccaa aaacttaacc actcaccatc tgttgaatta tgtgttttta tatcccaata
     4681 taacacccta tttattaacg atggtaagcc aatacatata ataagaattg ctattacact
     4741 tataaatatg gttagtatct tatgcttttc aatccaactt ttatttttat tgtttatatt
     4801 gctcattact ttccctccgt tctataatat atattttacc attttgtaca aaatggtaaa
     4861 atatattatg ggataaatat tatttggata taaactttcc ttttgtatta agatattatc
     4921 aatctcttat tttatctata atatgttatt tttaagccta aaaaagagat aactatttta
     4981 atcctagtta cctcttttta tgtta
//
LOCUS       DAYAOX010000243         4961 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.245, whole genome shotgun sequence.
ACCESSION   DAYAOX010000243 DAYAOX010000000
VERSION     DAYAOX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.245"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     misc_binding    283..514
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            622..2103
                     /gene="thrC"
                     /locus_tag="ACGI57_003294"
     CDS             622..2103
                     /gene="thrC"
                     /locus_tag="ACGI57_003294"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFG9718345.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2113..3006
                     /gene="thrB"
                     /locus_tag="ACGI57_003295"
     CDS             2113..3006
                     /gene="thrB"
                     /locus_tag="ACGI57_003295"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFG9718346.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3136..4191
                     /locus_tag="ACGI57_003296"
     CDS             3136..4191
                     /locus_tag="ACGI57_003296"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFG9718347.1"
                     /translation="MKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVISG
                     VQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEAVK
                     AGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIVVE
                     GPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSSSD
                     VKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAIRN
                     KFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAYKI
                     DKLSTVEDVINELISEL"
     gene            complement(4346..4858)
                     /locus_tag="ACGI57_003297"
     CDS             complement(4346..4858)
                     /locus_tag="ACGI57_003297"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFG9718348.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         1850 a          608 c          767 g         1736 t
ORIGIN      
        1 caaattaaag ttttgtaaac tttttttgta atatagtgta gtataaaatt gtaatattac
       61 agatatttaa tagttatctt ttaattactt tcattaaaaa cagcttcatc actatgaaaa
      121 tctaggtttt taaactttta aaaaagttaa aaaaatattt ttctaatact caatttgatt
      181 acaatttttt tacaatttaa aaaatatttt ttccaaattt ttatattaat acttgacttt
      241 gtctgtttta taaactagaa taattaataa tattaagaat attgaaaggc attgattagg
      301 aagagtaaat ctgaatcgat taccagagaa ggaaactact agctgaaatg tttccctaat
      361 tagaaggatt gaaaactacc taggagcttc tatccgaagt tatcttaatg attttagtag
      421 gaatagacgt ttttattact tgcgttaaag tatcaagtgg attttttaaa atcaatttgg
      481 gtggaaccgc gggaaacaga ttctcgtccc ttttttaggg atgagggtct ttttttatta
      541 cctaaattca gtttttaaaa tattctgtat ctaatttaat tagctaatta aaaatttaaa
      601 atatatggag gattataaaa aatgtattat tcaagtacga gaggcaccga agaaaaagta
      661 actgcatcac aagcaataat aaaaggaata tcaaatgatg gtggtcttta tgttccaagt
      721 gaatttccca atgtaaaaaa tgaacttata aatttagtaa atttaacgta ttctcagata
      781 gcattttttg tacttagtaa atttttatgt gattttactg aagatgaaat taaaaattgt
      841 atagaaaatg catatgatga aaagtttgat tgtagtagta tagctccatt aaataaagta
      901 aatgatactt atttcttaga actatatcac ggtccaactt tagcatttaa agatatggca
      961 cttactataa tgcctcatct tcttaaaaca tctataaaga aagataattt agagaaagat
     1021 gttgtgattc ttactgctac atctggagat acaggtaaag ctgcacttga gggatttaaa
     1081 gatatagata aaataaagat aattgtattc ttcccagaag atggtgtaag cccagtacaa
     1141 aaacttcaaa tgaaaactca aactggaaaa aatacatatg tagttggtat aaaaggaaat
     1201 tttgatgatg cacaatcagg tgtaaagaag attttttctg ataaagaatt taatcaaaaa
     1261 ctattggata ataattatat attatcttct gcaaattcaa ttaatatagg aagacttctt
     1321 ccacaagttg catactattt ttattcatat atgaaattag ttagcaatag tgatataaat
     1381 ttaaatgata aaataaattt tgttgtccca actggtaatt ttggtaatat tctagctgga
     1441 tattatgcta aacaaatggg tcttccaata aacaaattaa tatgtgcatc aaatgataat
     1501 aatgttcttt atgatttctt taaaactggt gtatatgata aaaatagaac attgaaatta
     1561 actacttctc cttctatgga tatattaatt tcaagtaatt tagaacgatt actgtttgaa
     1621 atatcaaata gaaatactga tattgtaaat aagcttttat cagaccttag tgataaagga
     1681 gtctacaaaa taagtgatga tatggaaaaa aatttagcct cattctatgg agaatattca
     1741 aatgaagaag aagttaaaag cacgataaac aatgtgttta aaaattataa ttatttgata
     1801 gatactcata ctgctgttgc atacaattgt tatgaaaaat ataaaaaaga aacttctgat
     1861 aacacaaaaa ctgttattgt aagtactgca agtccattca agttttctga agatgttcta
     1921 aagtctatag attgtgattt taaagacctt gatgactttg ctataattga tagattatca
     1981 agtatttcta aaatagatat tccaaaacct attaaaaatc ttaaaaatgc agaaatacta
     2041 cataaagata tttatgaaaa agatgaatta aaactagcta taaaaaaatt cttaaaggta
     2101 taggtgatta ttatgttaga gattatagtt cctgcaacaa gtgctaatat aggtccagga
     2161 tttgattgtc ttggaattgc gttaaatata tacaataaat tttatgttga agaaattgaa
     2221 agtggtcttg aaatagaagg ttgtgaagat gcttacaaaa acgaaaacaa cttagtttat
     2281 acatctatga agtacttttt tgatagagtt aaaccagaaa aaatacctgc tggtatcaaa
     2341 ataaaaatcc aaagtgaagt acctatatgt agaggtcttg gcagtagtgc ctcttgtata
     2401 gtagctggag ttatagctgc aaatgctcta tcaggtgcta atttagataa aaatcaatta
     2461 ctaaatatag cttcagaaat agaaggtcac ccagataatg ttgctcctgc tattttagga
     2521 aatatgattg tatctgttac tgataatgag aatatacatt atgatattat aaaaattcct
     2581 gaagaactta aattttgtgc aatgatacct aattttaagt tatcaacaga aaaagctaga
     2641 ggtgttttac caaaggaaat accatattct gatggggtat tcaatgttag tagagttgct
     2701 ttactaatat ctgccttact aaacaaaaac tttgacctac ttaaagtagc ttgtcaagac
     2761 aaattgcatc aagattatag aggaactcta attgagaact acaatgatat tgttgaaaaa
     2821 tctgaacaat taaatagtat tggagtattt ttaagtggag ctggacctac tataatgtct
     2881 ttaataaaag aaaatgatga tagttttgtg gataatatga aaaattacct acaaaaacta
     2941 aaatctgatt gggaaataaa agagttatgt tgcgattcta atggagctgt tttaaatata
     3001 atataattat cgtcttctat acaaaatata actactcttt tattataatt tgtagtataa
     3061 taattaggac taggtaataa tattaagtat tataatctag ttctttaaac atgtttacat
     3121 tgaggaggga ttatcataaa agagttaata attagagatt tagttgcaaa agttcctata
     3181 attcaaggtg gaatgggcgt tggtatatct aggtcatcac tagctggtgc agttgctaag
     3241 cttggtggtg ttggtgtaat ttctggagtt caaattggat atgatgagga agattttgaa
     3301 actaatacta taaatgcaaa tttaagagct ataaaaaaac atatttctaa ggccaaagaa
     3361 atctcaaatg gtggtattat tggtattaat tttatggtag ctatgaaaga atatgaaaca
     3421 tacgtaaagg aagctgttaa agctggtgta gaccttataa tatctggagc tggattacct
     3481 aataagctac catcattagt aaagggaagt aatgtaaaaa tagcacccat agtttcaact
     3541 gcaaaagctg ctaatgtaat tttaaaaatg tgggatagaa aagagaaaac tactgctgac
     3601 ttaatagttg ttgaaggtcc aaaagctggt ggtcaccttg gatactctaa cgaggaactt
     3661 gataacatag attctataga ctatgataag gaatttgttg aaattttaaa ggttgcaaat
     3721 acttatggag aaaaatttgg tagaaacata ccagtagttg ctgctggtgg tataacttct
     3781 agtagtgatg ttaaaaaata tatagatatg ggtgcaagtg gtgtacaagt tggtacaaga
     3841 tttgttgcta cttacgaatg tgatgcccat gagaacttta aaatggcata cataaatgct
     3901 agtgaagaag atgttcatat agttaaaagt cctgtaggtc ttcctggacg cgccataaga
     3961 aacaaattta tagaagaagt taaaataaat cgtccagaaa taaagaagtg ctataattgt
     4021 ttgattcctt gtaatccaaa agaaactcct tactgtattt ctcaagctct aatcaatgca
     4081 gttaaaggag atgttgaaaa ttcactttta ttttgtggaa atgatgccta taaaattgat
     4141 aaattaagta ctgttgaaga tgttatcaat gaattgattt ctgaattata atttaaagaa
     4201 aactaaatta aaattttatc aagctaagga gtttaaatac gttatttatt aatctttata
     4261 aacatattta tgtccttagc ttttttgtgt caatttcctt ttatggaaat tttaatttaa
     4321 ttagagatag tattaacctt tataactaat cttcttttac aagtacattg gttatttcag
     4381 ctatatataa agtatgataa tctttttgtg gataccatct tccatctata ccttcagcaa
     4441 taaaacattg aggttcaagt ttctgactat acattttttt gcatataata gccattttag
     4501 attctttaaa ataaggtgta tcatttaagt gttctatatt aaatccaatc tgtgatatct
     4561 tatcctcttc tctaccagat acctttccac aatatgaaag ctctttacga aaatcttcat
     4621 caaaaaatgt aagagaaaat ttatctgtat tatctataaa ttccttagta taacgctgtg
     4681 gtcttattac tacaaaagca acatcttttc cccacataac tccaagccct ccccagctag
     4741 cagtcattgt attcacttta ccttcatttt ctgctgttat aagtgtccaa tctttaccta
     4801 tcaatccaaa tggattattt ttaatctctt ctgccaaaat ctctttaaaa tcactcatat
     4861 ctctaataac ctctcattct cttatattta tattcctttt attttcgtga taaattataa
     4921 atgaagtcta gttttgaaaa taaaatactt ttttaaatat a
//
LOCUS       DAYAOX010000244         4943 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.246, whole genome shotgun sequence.
ACCESSION   DAYAOX010000244 DAYAOX010000000
VERSION     DAYAOX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.246"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            228..875
                     /locus_tag="ACGI57_003298"
     CDS             228..875
                     /locus_tag="ACGI57_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HFG9718349.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(1042..1611)
                     /locus_tag="ACGI57_003299"
     CDS             complement(1042..1611)
                     /locus_tag="ACGI57_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718350.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1616..2515)
                     /locus_tag="ACGI57_003300"
     CDS             complement(1616..2515)
                     /locus_tag="ACGI57_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9718351.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIIFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2526..2675)
                     /locus_tag="ACGI57_003301"
     CDS             complement(2526..2675)
                     /locus_tag="ACGI57_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HFG9718352.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2668..3252)
                     /locus_tag="ACGI57_003302"
     CDS             complement(2668..3252)
                     /locus_tag="ACGI57_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HFG9718353.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            3641..>4943
                     /gene="cdeA"
                     /locus_tag="ACGI57_003303"
     CDS             3641..>4943
                     /gene="cdeA"
                     /locus_tag="ACGI57_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HFG9718354.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIFALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKK"
BASE COUNT         1689 a          751 c          603 g         1900 t
ORIGIN      
        1 gtattagtgg ttatgtttta taagtatttc tttattcagt tatttattaa aaactaatag
       61 taaatcttat actaatcgca tatttatgtt ataactatat tgtaactatt ttcgtaatct
      121 taggaaaaga ttacttataa aattaacctt atacaataat atatcgtata atcttaatta
      181 gcataatagt tacaatatta aaatttaaat atgggagtga ttttagtatg ataagacaaa
      241 caccattaga gaaacgatat gacaagctag gtcctcatat tgtaacagca ctcaaaaaac
      301 gttattttga tgcttattac tgtaaaacta gaaatgaagc tttgcaacaa attttagatt
      361 taattcctca aaatcatgta gtatcatggg gaggatctga aacacttaaa gaaatgggcg
      421 tacaaactgc tgttagagag aaaggattta aagttattga tagagattta gcaaaaactc
      481 cagaagaaag aactgaaatt atgcgtcaag cacttctatg cgatactttt ttgatgagta
      541 gcaatgcaat tagtgaagat ggacaactct ttaatattga tggaaatggc aatcgtgtag
      601 ctgcaatgat atttggtcca aaaaatgtta tagttgtagc tggtatgaat aaaattgtaa
      661 aaaacattga tgatgcaata caaagaacta gaacaattgc atctccagca gttgttcagc
      721 gttttcaaga tgtcaataca ccatgctata ttaatggaag ttgtatagat tgcacaagtc
      781 ctgaaagcat ttgtgcatac atggttacta caagaatcag tcgtccagca aaaaaaataa
      841 aggtaatatt ggtaggagaa aatcttggtc tataaaatat acatacatgt aaatagctct
      901 ataaataata aaatcatatg aaataactta tttctaaaag tggtagaata aaagcttcta
      961 caagactttc attctgccac ttttatattc ttttaatttg tgtttctata ttattaattg
     1021 ctccaatttt catatatatt actaactatt tttattttca taattttttt gttcttcatc
     1081 aagctgttta taataatctt tattatagtc agctagataa tctataccac ctcctgtaat
     1141 cacatttaat aatttcattg cagattcttg attaacttta caaacataat tatcaccaga
     1201 tttaacaagc tctaatgtaa tattaaattc ttttttcttt gcagaatcaa tatctttcat
     1261 agttggattt ggcatcattt ctcctagttt aggaggattc ttttttaaaa tagaaaccat
     1321 atcagtattg ttaattttta aatctacagt cactttatcc ttattatttt ttatatctga
     1381 aatttcccaa gtcatatttt tagaatacat tttaaatcct tctttatctt cgtatataag
     1441 attacctaac cctgattttg ggaaatactt atcaacagta tctgcatctt gtgtttttat
     1501 tgaattaaac atactatcca cttcattttt tatcttatta tcattagtac aaccagctaa
     1561 caccagtact gatattgcaa acattataat acatagaata aatttcttca ttaaattata
     1621 tccccctata cttttttgca caaatattat tctctttttt atcttttgaa aaagttgtag
     1681 taattgcatt ttgtggacaa acttttatac attcaccaca cctaatacag tctggactat
     1741 ttacatcttt atatattggt atatcaagct tacattttgt ttgacatgct gtacaatttg
     1801 tacatttatc tttatcaatt ttaaatctat atattgaaat aggattaaaa aaaccataga
     1861 ttgctcctaa agggcatata tacttgcaaa atggcctata agtaatgatt gacatcaata
     1921 atatcaataa caaaatactt aacttccagc taaataaaaa tcctattgat tctcttaaat
     1981 tctcatttcc taaaataagt ggtattcctc ccaaaagtgt tcctgaaggg caaatccatt
     2041 tacaaaacca aggatttcca ccccctgcta catttacaat agcaactggc aaaataatta
     2101 caaatattat taagactata tactttagat attttagata tttatcacca taaacttttt
     2161 ttatctttac tggaaagggt actttatgta gtaaatcttg tacaagtcca aagggacaaa
     2221 gccaaccaca aacaaatctg ccaaaaaaag caccaaatgc cattaaaaaa ccaacaacat
     2281 aaaatgagaa tttatactct ctgcttgata aaactgcttg taatgaacct attggacatg
     2341 ctccaagtgc acctggacat gaataacaat tcaatccagg tacacataat tttttacttt
     2401 ctcctccata tatagttcct tctaaaaatc catttacata tccatttgtt aatgctgtaa
     2461 atgcaacttg agtaataagt cttatattat tatttacttt agataataac ttcattcctc
     2521 ttctcctaac caataccaat acactcaaga caaatcataa ttgctttagt taatactgta
     2581 tttgtttctc cacgatatgt acctaatgca ataaatataa tactaattgc aataatagaa
     2641 atgccttttt tattatcttt aataaagtta ctcattggct tttaccattt ttagtctttc
     2701 atctataatt tttttccaat cttccttact atttgcacct acaaccttct cacccacaaa
     2761 gtttcctttc ttgtctacaa aataagttgt tggaaactcc ataactcttt taaaatcagt
     2821 ttttttaagt tcttcagata cagtcaattg ttggtaagtt gcatctgact ttttcataat
     2881 gtctttaact aaatttttct cttcatttga aagaccagtt ctcatatcag ttttatcaac
     2941 ttctacaact aatcccttta tagctacatt ggaattatta ctctcatatt cttttgataa
     3001 tgcttcaagc tctggcattt cacccacaca tggtccacac caagtagtcc agacattaat
     3061 aagagtaagt tctttatttg aaaatatctc ttttgttacc ttatttccat caacatcctc
     3121 cgtatctaaa ttactaaacc attcactatt attcttgttt ttttctactg gcttagattc
     3181 ttttttgcta catccagaaa tcatacctaa aacacaaata catcctaaaa ctactatgaa
     3241 aaaccttttc atatctcaca ctcctagaat ttatatagta tataaaatcg ctgcttgaaa
     3301 taaaaataac ttatcttcta tattaaagta attatattta ataaataaga gctaatttaa
     3361 aaaaagatat acagttaaaa tctatttctt ttcagacaaa atcttatata tatataatag
     3421 cttgtaaatc ttaagattat atacatatat ttctaaagat tttcttaaga aacagttaga
     3481 ttttactaat ataagtttat taattagcat tatactaatt tatcttaata tgattttttg
     3541 ttgattttaa gctgtttatc gtataaataa aatttttctt gactatatag ttactatata
     3601 gcttattata tatagagagt aataaaagga ggtaaaattt atggaaaatt tatttacaag
     3661 aaaattcact acttttgaat ttctaaaatt tgtttctcct gcaattatat ccatgatatt
     3721 tatatctttg tacacaataa tagatggcat ctttgtatcg acattagttg gttctgatgc
     3781 tcttgctagt ataaatattg tactacctat aattaacctt gtttgtggat ttggaataat
     3841 gatggcaact ggtggaggtg ctatcgtttc tatacgtatg ggtgaaaata gacaggatga
     3901 agccaactct acattttctt ttatagtttt gttttcattg attgttggga ttttattcac
     3961 agtaatctca tatttcttta tcaaagaaat atctattttg cttggtgcaa cagataagtt
     4021 attaccatat tgtataactt atggtaaagt tatgatttta tgtacaccat tttatatttt
     4081 aaaatttata tttgagtact ttgcaagaac tgatggaaat tctaaattta gtttatttct
     4141 atcagtcatt ggtggtgtaa caaatataat attggattat gtatttatta aatattttgg
     4201 aatgggtctt ttaggagctg cagttgcaac tgctataggt attattttaa cttgtgtttt
     4261 aggtattatt tacttcttat ctaataaatc tacactaaaa ttaagaaaac caaaaaccga
     4321 ttttagactt ataagagata ctatgataaa tggttcttct gaaatggtta cagaattatc
     4381 tacaggaatt acaacattct tatttaatgt agtagcttta aaattagcag gagaaaatgg
     4441 acttgctgct cttactatag tattgtatgc tcatttttta atgacatcag tctatctagg
     4501 attcgctgct ggagtgtctc cattaataag ctataatttt ggtgctgaaa acagtgataa
     4561 attaaaagaa acatttaaac attctctaaa atttatattt gtttcttctc ttttagtgtt
     4621 tatttttgct ttagtatttg caccatttat tgttagggtc tttgtaagtc cagataacac
     4681 agtatttaaa ctagccttac aaggattaaa aatatttgca tttgcttttt tgtttgttgg
     4741 tataaatata tttgcatcag gattttttac agcatttcac aatggaaaaa tttcagctat
     4801 tatatctttt agtcgtgcct ttgtttttat aatcatagga atcataattc ttcctcctat
     4861 gttaaacatg actggattat ggcttacagt tccatttgct gaggttataa ccatatttat
     4921 atctattcta tttataaaaa aat
//
LOCUS       DAYAOX010000245         4942 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.247, whole genome shotgun sequence.
ACCESSION   DAYAOX010000245 DAYAOX010000000
VERSION     DAYAOX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.247"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            101..1198
                     /locus_tag="ACGI57_003304"
     CDS             101..1198
                     /locus_tag="ACGI57_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFG9718355.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRY
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(1689..2201)
                     /locus_tag="ACGI57_003305"
     CDS             complement(1689..2201)
                     /locus_tag="ACGI57_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718356.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            2754..3635
                     /locus_tag="ACGI57_003306"
     CDS             2754..3635
                     /locus_tag="ACGI57_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HFG9718357.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKSVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(3948..4727)
                     /gene="hisJ"
                     /locus_tag="ACGI57_003307"
     CDS             complement(3948..4727)
                     /gene="hisJ"
                     /locus_tag="ACGI57_003307"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HFG9718358.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNKILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLDLVKKYDIPLVLGSDSHCAEEINKYDII"
BASE COUNT         1822 a          706 c          514 g         1900 t
ORIGIN      
        1 tttattctat aatctagtta aaataacaca taatatttta atatttacac ataatcagtc
       61 attcaaaaat ttaaatttaa ctttttaagg aggctttaca atgaaaaaaa catatctatt
      121 cagtcctgga ccagtaatgg ttacagataa agtcagaaat tcacttcttc actatgatat
      181 atgccataga ggaaatgagt ttatggattt attcaaagat acacaacaaa aaatcaataa
      241 actatataat gcaacttcta attactactc attaattgta tctggctctg gaacatctgt
      301 aaatgaatgt gtactatctt ctatctttga caaagaagat gcagttctgc tagttagtaa
      361 tggtgttttt ggagaaaggc tagaagaaat attatctact tataatgtaa aaacatataa
      421 acctaatttt gaatgggcag aatacccgga cctagacatt ttagaaaaat acataattga
      481 aaactctgat ataaaagtaa ttgctatggt gttccatgaa actagttcag gaatgataaa
      541 tccagtccct gaactaggaa aacttgctaa gaagtataac aaaatatttt ttgtagatgc
      601 aataagtgcc tcagcaggtg aatatattga tgtggaagag tttaatatag atataataac
      661 aggtgttggt ggcaaagctc ttggagcatt tccaggttct gcataccttt gtgctaaaga
      721 gagtatttta caaaatatta aagaaaatca atgtaagaat gtgtatctta atctttataa
      781 acactataaa gttgctaaat catcttctca aacaccaaat acacctaatg ttactttaat
      841 atttgccctt aatgaagctt taactgagtt tttggaagat gacagtaaaa tagaaagata
      901 caaagaatgt tctgctattt taaggtatgg tatggaagaa ttgggtctaa catttttact
      961 tccagataag tatatgtcta atacagtaac atctgtattt ctacctaaag aaatcaatgt
     1021 aaatgagttt atattagggc tagaaaggga aagtggctat gttgtctatc caggaaaagg
     1081 caaattccta gatgctaaca tattccaagt agcaaatatg ggagaaatat atccagaaga
     1141 ttgttataaa ttcttagata tcttaaaatc aaaattggag ataaaaaaag ttatataaca
     1201 gtttgtttgt aaaatcctac aatatatcag tataaattaa atagactgat ttttttaaat
     1261 tttcctataa taaattatat tttatatttt tgtataaggg tgaactataa tatgtaaagt
     1321 tcacccctat ttcaaatctt tttccttcaa tataatcttt ttcttttaat ttattttata
     1381 tttttgacgc aatcgttttt tattcaatca ttttatattc ttgatacaat cattcttaca
     1441 cttaattatt ttttgttttt gacacaatcg ttttttgttg aatcatttta tattcttgat
     1501 acaatcattc ttacacttaa ttattttttg tttttgacac aatcgttttt tattcagtca
     1561 tttaatattt tcaacacaat cattcttaca ttctattatt gtttgttttt gatacaactg
     1621 tttttattca gtcatttaat attctcaaca caattacctt ttcattcaat caaagtaatc
     1681 tttaagcatt atttttcaag ctcatcaaag taattatata cgataacctc acctgtctct
     1741 ttatctgtag tctttataat gcccttagct ccatttacct cttcttttgt aaattcatca
     1801 tctaacacaa ctttgaattt atcttccttt ccataacctt gttgaaattc atctctcaaa
     1861 attgcatttt tttctgcttt tggcttcaac tcaaattcaa ttccagattt catttcatca
     1921 aaataattta atactacaac ctcattattc attttattag ttgtctttat aatcccttta
     1981 gctccattta cttcttcttt tgtaaattca tcatccaatg ttatattaaa tttgtcttct
     2041 tttccatatt cctcttgaaa atccctcttc atttcattgt atttagtttc aaactcttta
     2101 ctcttgttta attccatagc tgatacattt gtcaatgatg ctccaattgc cattactgtt
     2161 aatgccatta ctataccact tttaaatttt tttactttca tatcttacct cctaaattaa
     2221 atgttctcaa tgaaattata taaagaaaat cctaagtttt acagtttcat tcttgtaact
     2281 aaaattacta ttatgtaacc aaactttttg cttcaactat tccttaaaag ttgctttttt
     2341 gaggaatagt tgaagttaaa taattagatt ttatctatat ttatagattt aaaatcaaat
     2401 ttatccatat aaataaatac ttatttttgc aaaataaaaa tttttaatta ctccaatcct
     2461 tcttatttac ttaaaatcct atgataagaa atttaaaagt ctttaccttc tttaacaata
     2521 atttatttac ttttaacata caaaatatag agagtatatc aaaactattt tactgaatat
     2581 tctaaaaata agtaccccct attagtatat atatttacat attaaaaaaa agttttaaat
     2641 aaagttatat tttttacaac agttttgtaa ttaaaaacat attgaaggta aatttaaagg
     2701 tactatatac tataaatatg tcgacacaat aaatttaaaa aggagaacta aatatgaaaa
     2761 aaagattaat aataatgata ttgtctgttg tcttagtact atcaagtata cttacgatat
     2821 ttgcatattc aaatattaaa tataataata acaaattgat atattctaat atgatagata
     2881 aaaaaactca aaatagtgta aaggaaatcc taaaagaaaa taaaattaat gaaaaagaca
     2941 tagatacatt tattaaatca gtcaacaatt ataataaatt gcaagttaaa atacttcaaa
     3001 ataatataaa tatatctaaa tctggatatt cttcaatcaa tgcaaaacaa gttccatata
     3061 acctagaaaa attgcaagat aattgggtga aaaaattccc tgattatatg gatgtaaatt
     3121 gtagaataac tgcttttaga ctttttaaag attttataaa ttcaaataag aaatttactg
     3181 gagactctat tgatttgaat gtcgacttgg acactataat gaataataaa gatgctaaat
     3241 ttagcaccaa agatgtagaa aaatttatca acttcttttc tgcaatacca gctaaagata
     3301 cagatgatac aatcaaaatt gcagaacaaa taaaaaatga atggaaaaaa agaaaaatat
     3361 cttttaaaaa taataaaaat atatctatta taaatggatt tcttcgttat ccagaaacta
     3421 aaaatgtatt tataggtcat acaggcatat gtattaaaac taaaaatggc attctatttt
     3481 tagaaaaata cggtgttaca tcaccttatc aagttactaa atttaaaaat aaagaagatg
     3541 taaaaaatta tatgtttaat agattgaaga tgtcagaagg tgaaattgaa ttacctgacc
     3601 ctataataat ggaaaacgat aaacttatga aataataact ttaaacgcaa aatagtattt
     3661 ataattacta agtcaatata atagagaagg tctatagatt tgtgtaaata caagcttatc
     3721 tacaatctta tataagaatg tttatataaa taagatagat taattctaag tcctattatt
     3781 ctattgaatt ttattattat tttatttaga ttctattgtt attttactta gaacttatta
     3841 ttattttgtt taagttctat tactattttc aatcatataa atttacaaaa gactgtacat
     3901 aaatttattt tatacaaact tatctacagt cttttgtgtg aatatatcta aataatatcg
     3961 tatttattta tttcctctgc acagtgagaa tcagaaccta aaactaatgg aatatcgtat
     4021 ttttttacca agtctaataa gtatccatct atataaggct ctttgcagta ctcttttcta
     4081 tttccagaaa cattataatc aagttcataa cctttttctt taattaactt tattaactct
     4141 tcaagaattt tatttccttt ataatcgtat ggaaatacct gattaaactt tctaactaaa
     4201 tttaaatgac ctattctttt tggtttatgt atacctaaat ctgaatttac agcttttatc
     4261 aatgtttcat aatacttatt ataaacagct tcaatacttc ctaatttgtc gattaaattt
     4321 tgaaactcct ctacactgta atctacacaa taataatcat catcaatctt aattatatgt
     4381 actgataaaa gagaatcctc taattccttt ccatatttat ttaaattctc ctttatccct
     4441 tcttcgtatc cttctataaa atccacttcc gacccaacat ttatcttgat tttaccctca
     4501 tacttttctt tcaacttctt gacatcatta aaatactttg gtaaatcttc aatactcata
     4561 caactatcat tttcaggagc agggtctcta aatccatttg catacggaaa atgttctgta
     4621 aatgtcattt ctgttattcc aacatccatt gctctttgta catatttttc aaaatcatcc
     4681 ttacttccat gaggacaata aggtgaatgt atatgaccat ctttcataaa taattcactc
     4741 cttttttaaa gaaaaaatct aatataaatg cataaatctt ttaacttcaa aataatattt
     4801 ttaatcatgc taatttttat tatatctaat attagagtaa ttttaaactg ccttttaagt
     4861 atcttatcat tagtactatt ttcaacttaa cattaatatt attttttaac ttaacattaa
     4921 tactatcttt aaatagtaat ac
//
LOCUS       DAYAOX010000246         4883 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.248, whole genome shotgun sequence.
ACCESSION   DAYAOX010000246 DAYAOX010000000
VERSION     DAYAOX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.248"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(365..1168)
                     /gene="proC"
                     /locus_tag="ACGI57_003308"
     CDS             complement(365..1168)
                     /gene="proC"
                     /locus_tag="ACGI57_003308"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFG9718359.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEKFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(1493..3862)
                     /gene="hypD"
                     /locus_tag="ACGI57_003309"
     CDS             complement(1493..3862)
                     /gene="hypD"
                     /locus_tag="ACGI57_003309"
                     /EC_number="4.2.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase"
                     /protein_id="HFG9718360.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(3867..4775)
                     /locus_tag="ACGI57_003310"
     CDS             complement(3867..4775)
                     /locus_tag="ACGI57_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase activase"
                     /protein_id="HFG9718361.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
BASE COUNT         1468 a          926 c          522 g         1967 t
ORIGIN      
        1 agtaaaacat tataggtatt ttaaaactta ttagcgaaga aaatatttgt aacttttatt
       61 accttatgaa tattatttca aatttaaaaa ggctataaat aaatttattt ttaaataaat
      121 ctacttataa cctcacaata atacttataa attttatttt ttcacaatga tatatctcaa
      181 atatagttaa aaatatatct atttaattat taatatttaa gtaataaatt atcactcaat
      241 cattaaattc ctttttatag taaattactc ttattttact tataccaaat acatttatgt
      301 atagatataa ataagcacta aaaaaacaat tcaagaaacc atatagtaaa cttaattaca
      361 tctgttattt gctcatatct ttagacttat ctatacaagc aacaatagat tttataactg
      421 ctgctctaaa gccttcttct tctaatacct taacagcttc tatagtagtt cctgctggag
      481 aacaaaccat atcttttaat tcacctggat gttttccagt ttcaagaacc atcttagctg
      541 accccattac tgcttgagat gcaaatttat aagcttgttg tcttggcatt ccagctaata
      601 ctgctgcatc tgccatagct tctataaaca taaatacata tgcaggagaa gaaccactaa
      661 tacctgtaac tgtatccatt aaatattctg atactatttc tgcctcacca aaagattcaa
      721 acagagcctt aatcatattt aactcttcat ctgttacatt actattttta caaagagctg
      781 acattccttc gccaacaagg gctggagtat taggcattac tcttactatt tttttatctg
      841 aacctataaa actttccaga ctttctatag ttttacctgc tgctatagat actattattt
      901 tatttgcatc tattaattct ttaagtggag ttaaagcctt ttcgcatata ttaggtttaa
      961 gtgctactat taccacatca gaatttgtaa cagctacctt tgaatcagtt gtagtattta
     1021 ctccaaattt ttcttttact ttatctaagg caacttggct taaatctgat acagttatat
     1081 cttctgcctt tactaatcct gactgtacta tgccacctat cattgcactt cccatattcc
     1141 ctgaaccaat aaatcctaaa gttttcatat caaatctcct tttttctatt agttttattt
     1201 taaagtagct aaattgactt tattttaact gcttaaaatt atcttatttt tataaaatat
     1261 aaccttatta taactataat aaattattct tttgtatgta ttacttacta atatgtgtat
     1321 gatttactaa atataaattt aacgattttt tcattaaact taacgagcta acttcctatt
     1381 tagaaagtta gctcataatt aatctaaaat tttaaaaatg gaatcaaacc atttatactt
     1441 tattatatca gttacgattt tatatagtaa gaagttttat ttacaagcaa acctagaatg
     1501 tttgctcagt tcttcctatt atctcatctt gtagtgttct acttaaatta ttgaaatggt
     1561 cactatatcc tgcaactcta actattaagt ctttataatc ctgaggattc ttttgagcct
     1621 ctaacagtac atttttatca aatacattaa attgtatatg atgaccatcc atattaaagt
     1681 atgctcttac tagattagcc atattatcta accctttttc tccttgaact actgatggtg
     1741 caaatctttg atttaataat gttccaccag tttttatgtg gtccatctta gcacaagatt
     1801 ttataactgc tgtaggtcca ttagtatctc cacctttttc aggtgaaatt ccctctgaaa
     1861 ctggttttgc acaaagtcta ccatttggac ttgctcccat aatttctcca aagtatatat
     1921 gacaagttgt tggtaacata tctactctgt attctccacc acaaacagtt ggtctaccag
     1981 ttacctcatt gtaatatgca ttgaatatct cttgcattat ttcatcagca taatcatcat
     2041 catttccata tttaggagtc ttatttgaaa ctaaattaag tatagcttca tatccttcaa
     2101 aattagcatc aagtgcttta agcaatgtat ccatatcgaa ttttttctta tcaaatacat
     2161 tatattttat tgctgttaaa ctatctgtta tagttcctat accaacacct tgtatatatc
     2221 tagtgttgta tcttgctcca cctctttggt aatccttgcc tttttctata caatcatcaa
     2281 ctattgtaga cataagtgga caaggcatgt gttttgcaca tatattttct atcacagcat
     2341 ttccttctat ttttatgtca atcatataat gtaattgctt tttaaatgct tcaaatagtt
     2401 cctcataaga ttggaaattt ctagggtctc cagtttcaat acctatttgt tttttactaa
     2461 ttgggtcata tccattattt aatgttaatt caagtatttt tggaatgttc atatatccag
     2521 ttagaacata tgcttcttta ccaaaacatc cagtttcaac acaaccactg gttccaccaa
     2581 gtcttgcatc ttctatagtt tttccagctt ccataagctc ttgaactatt gcttcagtat
     2641 tataaaatgc tggttgaccc catccttttc ttgatatttc acaagctctc tttaagaatt
     2701 tttgaggagt ctttttactt atttgaacat ttgaactagg ttgtatcaat ttcatttcat
     2761 ccataacatc aagtattata taactaactt cattaacacc atcttgacca tctggattta
     2821 ttccaccagt gtttatattt gcaaaatctg tatatgtgct actttctttt aatgttatac
     2881 caacttttgg tggtgctggc tgattgttaa atttaaccca taaacattca agtaattctt
     2941 gtgctctatc tctatctagt attccatttt ctacatctct ttcatagaaa ggatttagat
     3001 gttggtcaag tcttcctgga gtaaaagcat cccatatatt aagttcagta gtaaccccta
     3061 tatgaacaaa ccaatacatt tgtatagctt gatgataagt ttctggtttg tgagctggta
     3121 caacatcaca agtttcagca attagtaata aatctttttt cttagcttca tctgtttctt
     3181 cttctgccat ttgtcttgct ttctcagcat atcttttacc taatataacc attgcatcac
     3241 aagctattgc catagcttct aaatctgctt ttttattata tgcatctaaa tcattaagaa
     3301 aatctagctc ttttaatctt gcttctatgt ctttctttaa atctaaaaat ccttttttat
     3361 atattgtatc tccacaaact gtatgtcctg gagctctttg ttccataaac tctgtaaaca
     3421 tacctgcttc atatgctgct aaccactcag gtgtcattgc atttattatc ttatctcttg
     3481 tttgtctctt tttccaaaaa ggaattattt cttctttatg tatttttcta gcttcttctg
     3541 atacgctaaa atttattata tctctattgt gcattacttc taaatcttcc attgtatgac
     3601 aacaaatttc tggatatgtt ggtgctccat taggagaatc acctttttca cctactatta
     3661 attctccatc atttatgcta agagttctat tctcaatata atgcttaaat gataaagcac
     3721 gaagtacagg aatctctaca cttccctcat attttttata agcttctgtc ataagtactg
     3781 ctctttcaat agatatgtgt ggttctgcat taatactttc ttctcttaat tttttagttc
     3841 tctcaaaagt tcctcttgcc attttattac cctccaatct tagtattgaa attacttgct
     3901 tcaaacagtc tttttatttc ctctaatttt tcttctgatg gtctttgtag ttcttctcct
     3961 tcatactttt tataaatttt atcatacttg tgtttaccaa tattatgata tggaagaaga
     4021 cttactgctt gtatattcaa aggctttaaa aactctatca tttttttaac ctctaaattt
     4081 tcatcatcta catttactcc tacaattaat ggtattctta tatttatgtt agcccctaat
     4141 tcacttaaaa tcttaacatt tttcagtatt aagtcatttg attttcctgt aaattttatg
     4201 tgcttatctt catcaataag cttaatatca tataaaaaca aatctgcaca tttagcaact
     4261 ctttcataat tttctgactt tgcatagcca catgtatcaa ttgctacatg gattcccttt
     4321 gatttgcaca tgtttattac tccacaaatg aagtccatat cttgagccat tacttctcca
     4381 cctgatagag ttactccacc acctgattct tcatagaaaa ttctatcctt ctctatctct
     4441 ttaaccaaat ctcttacaga atactcttga ccaacaatct ctcttgcatt gtttacacaa
     4501 tagtctaagc aagtttcaca aaactcacac ttatcttggt ctaagcatat ttttgtttca
     4561 cctttgtaaa ttgccttatg agggcatata tttatacatg cttcacattt tgaacatctt
     4621 tcttcattat acaaaacttg ttttgtatat gtttgactct ctggattatg acaccaaaca
     4681 cattcaagtg gacacccttt aaaaaatact gtacttctta tccctggtcc atcatgtatg
     4741 ctacattttt gtaagtttat aactaatgga ttcatgattt ttcaccccta aataatatcc
     4801 attccatttt ttcattttta tgcaattcat cttcatagaa tatacctatg aaaagcattt
     4861 ctacaattac ataatactat att
//
LOCUS       DAYAOX010000247         4860 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.249, whole genome shotgun sequence.
ACCESSION   DAYAOX010000247 DAYAOX010000000
VERSION     DAYAOX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.249"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(64..501)
                     /locus_tag="ACGI57_003311"
     CDS             complement(64..501)
                     /locus_tag="ACGI57_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFG9718362.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSIPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(758..907)
                     /locus_tag="ACGI57_003312"
     CDS             complement(758..907)
                     /locus_tag="ACGI57_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HFG9718363.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(920..1150)
                     /locus_tag="ACGI57_003313"
     CDS             complement(920..1150)
                     /locus_tag="ACGI57_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HFG9718364.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(2009..2230)
                     /locus_tag="ACGI57_003314"
     CDS             complement(2009..2230)
                     /locus_tag="ACGI57_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HFG9718365.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2810..3067
                     /locus_tag="ACGI57_003315"
     CDS             2810..3067
                     /locus_tag="ACGI57_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFG9718366.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            3536..4774
                     /locus_tag="ACGI57_003316"
     CDS             3536..4774
                     /locus_tag="ACGI57_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HFG9718367.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYITYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1855 a          493 c          678 g         1834 t
ORIGIN      
        1 taattattaa ataggtcaat aaatttgcaa ttagtgatat taataaatta ctttagattt
       61 ttattaaatg tttagctttt ctttaatatt ttttatataa cgacgattta taataacttt
      121 ttcgttgttt tttaaagtag cttgcatgcg attatgtgat attactttta tactttttat
      181 gtagtccata tttaaaatca tggatttaga tattcgtgag aaagatgtat tagctaataa
      241 ttttgataat ttatatattt ttaaatcaat ttcaaataca tcatcttcat aataagcaaa
      301 tgttttattt ccaactgtct caaagtataa aatatcacag acattgagtt tataaatctc
      361 tcctgatttt ttacctttta tatcaagtaa cggtatactc aataatgaaa ttattttttc
      421 aatgttatta tctagagcag aacattttat ggttatttca atatcatcac aattaatacc
      481 ttgttcgaca tgtattttca ttaatcatca cctcatttta ttactgtcat tagaatagca
      541 ttatatctta ttgagtccaa ctagatgcgt tctaaatttc aaattaagca tagtgaaatt
      601 caaaaataaa acagtgaaat tcaaatttga ggtggtaaaa ttcaaaaaaa tactatggat
      661 atttggataa attatccaaa tatcctagta tttcacatag aatatgtcta attattttac
      721 aaatagacct attttatgac ttacatatat tgtcaaatta cttcttaaat atcaaaatat
      781 ttttggataa tgtatcaata ctgtttgata aagtatttaa tttaccctca attctaacca
      841 acaagtacat ggatagagct ataggaaaac caacattggt tattaacagt tgtaaactat
      901 catccataaa attagccccc tattctataa ctaagtcata aggagtggta tttgtttcca
      961 caaccttggc ttcaacaaca gaagctaggt caaatcctcc aaccgaaaaa atatttttag
     1021 atactatcaa atccatgaca gattttatat tttcttcagt taaatcatcc tttggatttt
     1081 ctatagaaaa agaaacttgt ttatccttta catttttaaa gctcatgact aattttttct
     1141 taatttccat ataattacct ccgatataat aatttatatt ttaattgttt gcttgttaat
     1201 attggtattt taattatcaa tattaatgtt ttgcttgtta gtattaatat ttcaattgtc
     1261 aatattaatg ttttgcttgt tagtattaat atttcaattg tcaatattag tgtcttgctt
     1321 gttagtatca gtgtttcaat tgtcaatatt agtgttttgc ttgttagtat taatatttca
     1381 attgtcaata ttagtgtttt gcttgttagt attaatattt caattgtcaa tattagtgtc
     1441 ttgcttgtta gtattaatat ttcaattgtc aatattagtg ttttgcttgt tagtattagt
     1501 atttcaattg tcaatattag tgttttgttt gttagtatca gtgtttcaat tgtcaatatt
     1561 agtgttttgc ttgttagtat tagtatttca attgtcaata ttagtgtttt gcttgttagt
     1621 attagtattt caattgtcaa tattaatgtt ttgcttgtta gtattagtat ttcaattgtc
     1681 aatattagtg ttttgcttgt tagtatcagt gtttcaattg tcaatattag tattttaatt
     1741 gttgactcta gttattgctg atatagaaaa tatttaattt atttcaatta attagattaa
     1801 agtagggtag catctaaaat agctaacatg tttaagttga aatacgttta atatattaat
     1861 ttttcaaagc gttaatatat taaagttata accaaaattg aagttataat ctgccaaatc
     1921 tatagcgtat taagtgcatt atataactta atcttttata taaagagtta attttttaat
     1981 ttgtaaagca gagtacgttt aaaagacact atttagatag tgatgtacta tctaatttta
     2041 aaatatctac aatagggttc tcttgaagac ttgctattgc ttctgcaaca gcataaacat
     2101 cttcatttaa tgcctctgct tttacatttg caaatgattt acttttaaca actgccttac
     2161 ccttatcatc aaaaccagca tccaatttta gttttatact agataaattt tttgttgaaa
     2221 caacttccat aaaattacct cctaaaaatt taatttcaaa gatttgtcga ctttctttgt
     2281 tagctataat actatcatct aagataatat cggagttaaa cagtaatttc taaagatttt
     2341 ttgtgtaaat ttttatagga aaaattttac ttttttgctg atgaaaaagt ttacaagtta
     2401 tttgtgaaga tatgttctta gtgttttgaa atagtatttg tatatattca tgcttagttg
     2461 agatagtttt gtatggattt tcatatgtgt agagttaaat gttaggtact aaagtttatg
     2521 ttaaattaaa tgttaaagct tatgttaaaa attttattaa atttaatctg taaataaaaa
     2581 agttaaaagt ccaatattaa cattttactt ttaaaccatt aaaatcttta ttaaagctaa
     2641 caaagttata agttatgaga aattttattg aagttaatac aaaaattaat tttaaagcta
     2701 agaaattttt tattagattc aataaaacta ataaatatag tttagtcaat aatgtattaa
     2761 ataaaaactt tgttaagtct ataaaaatat cttaaaggag gtgtaactta tgaagataaa
     2821 agtggaggat ataccagttc aatttcatgc aatggtagaa ataatcggaa ttgataagtt
     2881 catagaggtt gccaagttgt atggaggaac aaatacttat atacctacgt ataaggggct
     2941 ttttagatat gctagaaata gggatatagt aaaacaattt aatggagtca accacaatga
     3001 attggctatt agatataata tgtgtgtaag caatattaag cgaatactca atgaaaatag
     3061 tgtttaaata taataaaata atgtttaaac ataatgaaaa tagccttgtt aaacgtaaga
     3121 gtaaagacaa agaagatgtt ttataaagtt tttttagaca aattaactaa atttactact
     3181 tattaattta aatacattgt atattctata aaaaaatttg ttaaatactg gagatatatt
     3241 gagaatacaa agaaaatggt cgaaaatatt tgttggctaa aaaatagata aaatgataca
     3301 attgcttgta taaaggggaa aaataaataa ataaactata taaataaaga aatagcaatt
     3361 aatatgacat aatatattta taaaattaag aatataaata gatattttaa gggattagtt
     3421 gttaaattag gaaatattaa aaaaaatata tatttatatt taaactattt tgttatttat
     3481 ataaaaatac ttatctagtt actagcaatt attagtagaa atgggggaaa tagagatgaa
     3541 taagggattt tacaaattaa attcagtggt attgaacttt gatattaaga atgatgttaa
     3601 caagataaga tttaatcatt atataatgag aaaagaaaac atacaaagac ttcaaggaac
     3661 tctgccaaac ggacaatttt atatgacagt gagaagagct gctacagatt tacaactttc
     3721 tatttctact ataagtaggc ttgtaaaaga gtttttggat ttagggataa taagattagt
     3781 gtctaagggt gtaaaaggta atcgttctgt atatagttac gtaagtatag agtttaatgg
     3841 aagtttattt gatgatagta aatatatgga acttgactgt gtatgtaatg atgaatttat
     3901 tgataatagt ggcatagagt ttgattgtga tagcaaagat aattctttta atggagatat
     3961 aaatgtagaa actgtgcatt gcattaatga gtatttctct gatgatgatg tagagcataa
     4021 ttataacaat ttccataaca aagatactat aaaggataaa gccatttcta atattagcac
     4081 tatcaaaaaa tatattgatg aatcttttga tacaaataaa tcagaaaatt ctaacttaca
     4141 acaggagtca ataaatttca actttaatga tacatgcaat gaaatagata aaagaacgaa
     4201 taatagtata gaaaataatc aaaataaaag gcctatgaga ttgagtgaca tcagtctttt
     4261 aagtgataaa ggtatgacaa ataaaaagaa ttactttaat cataatcatg gtatacaaaa
     4321 agggactaaa tctgtaacaa agaaaaaaga atccttaaat aaatttaata aaaaaaatat
     4381 acagcaaagc attataaata tgctaaataa aaaatcaggt aagaatttta aaatcgattc
     4441 tccaataagc ataaaactta tcaatgaaag gctaaaggaa ggctatgtct tagaagattt
     4501 ctacaaagtt attgaggtaa aagtggctaa gtggaaaaat accataatgg aaatgtatat
     4561 aagaccagaa actttgttta gctataagtt tgaatcctat gtaaatgaaa acatatctat
     4621 gaaaagtcaa agtaatgttc acccatgtaa agaaggcaat agtaatgata gcagtaatta
     4681 tatacattct gattatataa catatgatgg cagtgtgcgt gagagaaaaa aatatgagaa
     4741 tgtaagatac tggaatccta tatgtgaaat ttaattagtt taaaaatgta tttatgataa
     4801 ataagatatt aatagagaat agtgagctat agcaaatgat atatcaatag ttggtagtaa
//
LOCUS       DAYAOX010000248         4839 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.250, whole genome shotgun sequence.
ACCESSION   DAYAOX010000248 DAYAOX010000000
VERSION     DAYAOX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.250"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            910..1572
                     /locus_tag="ACGI57_003317"
     CDS             910..1572
                     /locus_tag="ACGI57_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718368.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1581..4088
                     /locus_tag="ACGI57_003318"
     CDS             1581..4088
                     /locus_tag="ACGI57_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9718369.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            4419..4763
                     /locus_tag="ACGI57_003319"
     CDS             4419..4763
                     /locus_tag="ACGI57_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718370.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
BASE COUNT         2136 a          424 c          706 g         1573 t
ORIGIN      
        1 ttatatatac tattttatat aactatgcta aaatttaatt gaataaatta aaaatataat
       61 gaaagtttag ttttaattca tttatttaat aaacatttca agtaaaataa tgtttaaatc
      121 agaaatataa ataaatatta ttataatcgt tattaaagac caaaaatccc accttagaag
      181 tttgtatcat atgaaacctt tatagattat gaaagcaata atatagtgta tgatatataa
      241 aataagttat aaaagtattt ttttagatat tcaaaaagac attgtaagtt atattaataa
      301 tatgggtatc aacgaatttt ggatataatg agtttttcaa cttaaagtta tgtaataatg
      361 aatgtaaaga atatttaatg aaatcatttt gttggttatg tctagctgaa tacaataatc
      421 ttttagataa agattttata tttgaagata ggtgtgaaaa aattaagaag atattaacat
      481 cagcagttta gaggcaaagc atgtaaagta aagtctgggt aactaaaagt agtaatttag
      541 attgtaacta ttgttataaa tatgaaaatg aggaaacagg aaatataagt taggatgtag
      601 aaaataggtt actaaataaa aagcttaatt atataatcaa agaaataggt atatactgta
      661 caatttcatg gtgaagaact tttcctaaat aaaaaggttg gtaaaagaga tataagaaat
      721 aaaattcaat tataagatat ggtatgataa ctaaaggcac tatatggagt gaataggcgg
      781 aagaattttt tgaaaattat aaaaatagtt tctgttgcta catatcaata agtatagata
      841 gagattgcaa aatccatagc aaaaatcgaa tttataagtg taaataacat gtaagacaag
      901 gagtaaatta tgagtatttt agagataaaa aacctaaata aaatatatgg taaatcggaa
      961 acagaggtga aagcgttaag tcaaataaat ctaaaaataa atagtggaga atttgttgtg
     1021 atagtaggta aaagtggaag tgggaaaagt actttactac acataatggc aggacttgaa
     1081 actccaacaa atggtgatgt gattattgat aatataaata tatcatctct tgatgaaaag
     1141 aaaagatctg cattacgtaa agaaaagttg ggattaatat ttcaatcata taatttgatt
     1201 ccagttttaa ctgttgaaga aaatataaaa ctaccaacta taaatataaa aaataaagat
     1261 gaagcttata ttaatgaatt gatggagtta ctaggaataa aagatagaag aatgtatttg
     1321 ccaaatcaat tatcaggtgg gcaacaacaa agagtagcaa tagcaagggc tcttgtaaat
     1381 aaaccatcaa ttgtatttgc tgatgaacct actggaaatt tagacacaaa gacagagtca
     1441 gatgtattaa gtttattaaa agaatctcaa aaaaaatata atcagacaat aataatgata
     1501 acacataata tagatataac aaaatatgct gatagagtta tagaaataga agatggattt
     1561 ataagagagt aggtagattt atgaaactta attatattgt aaaaaaatat ataatgggaa
     1621 ataagaaaaa tacattatta ataattgtaa gtattgtaat ttcaacagcg ttgtttttaa
     1681 ttatgaatat aataagtgaa gatgcgacaa acttaatgat aaatcaagct aaaaatgaat
     1741 ttacattaaa acatgcacaa tatatcaatc caaaagacga agaaataaaa tatatagaaa
     1801 ataacacaag tatagataaa gttggaaaat caatgctttt aggaattagt gatattggta
     1861 aaggtcaaac actacaaatt ctatctcagg ataaagttgc tgaagaacta aatgatattt
     1921 acacactaga aaaaggtaaa ttaccagtag ctgaaaatga aatcgctata gattcatggt
     1981 atattaaaca aaaaaagata aaaaacccta taggaaaaag gataacatta gattatagaa
     2041 gacaagggat agatcaagaa gatttatata ctggggaaaa agaatttaaa ataactggta
     2101 tcttaaatag taatcctatt ttaaaagctc aaggaacatc aattggcctt ataagtaacg
     2161 attgtgctat caagaatatt cctactaaaa ataaatatga tcaagttatg tttactttta
     2221 aaaaggaaaa aaatatacag agacaatcac aaaaacttat taaaaatgga aatttgaatg
     2281 agaataacat ttattttaat aatgaattat taatagctat atctgatagt atgaatttaa
     2341 aaatacctta tataatagtt aatatagtac tagcactagc aactatatta ttaatttata
     2401 atatttttta tatattagta tcaaatagaa taaaggattt tgggatattt agggctttag
     2461 ggtttaatcc aaatgatatt tttaaaataa tgattttaga agtatctata tactctatta
     2521 taagtatttc tataggtcta attttaggtg ggataatagc tagtttatca agggagtatg
     2581 ttataggagt aatatacaat gtcaactata taaattctat aaaaaaccag aactatataa
     2641 acacatacat aatttctata ttattaagtt taggaactat aataatatca gtttcaaaac
     2701 ctcttatgtt atcagttaaa actgatccaa tgatttgtat aagaagaaat aatgaaaaag
     2761 tggatataaa acaaaattcc tttataaata aatttatgat aaaacttttc aaagactatg
     2821 gtaacatagc atcaaaaaat atacaaagaa ataagaaacg tacaaattta tctatagcat
     2881 caatggtaat tatatttttc ttaatggcaa caatatacac aaagtctact agtaactttt
     2941 taagtgatgg tgtattaaaa tattggatac aaggagatta tttattacat aatattgata
     3001 taagtactat acgttcaaat aataaaggtt atgataaaaa tgtactacaa gagataaagg
     3061 caattgatgg tgtaacaaaa gtaaactcat ataggcataa gtggtttaat ataaaaattg
     3121 atgataaaag tataaataag aactctgatt attggaaaaa aaataaagat aatattgaag
     3181 ctagatcaga agtaaaagat ggaattaagg tatataataa ttcttttgaa tttttaggaa
     3241 tagaagatac agatatacta gaggatttct tgataaatgg aaaggaaaat atagataaat
     3301 ttagtaaaca accatatctg tatataacaa agaaatccag tgattcgctt aatataaaaa
     3361 aaggagatag aataaaagta tactttgata ttatagattc aaaaacaaat aattttacca
     3421 aaacaatatc tcaagaattt atagtatgtg gaattataac tacattacca gtaacctcac
     3481 aagtgggagc tgaatttggc ggagtaatat caacaaatca atttaataag tttacaggaa
     3541 tatcatctta tgaaagattt gacatatgga cgagtaaact agccaatgat aaatatgttg
     3601 aaagtgagtt aaacaaaata acagaaaaaa caaataaagg aattttaatt ccatacaaag
     3661 ctgaaacagc tgaatatgaa aaaattgata atcaaaaaac actaattatg gtattagtaa
     3721 ctggaattat agttatatta tctttattta attgttgcaa tactatagtt actagtatta
     3781 atagcagaag tagggaattt gcactattta gaggaatagg aatatcaaaa catgaaataa
     3841 ataaaattgt taaattggaa agttatatat atattatagt aagcttttgt atatctataa
     3901 tacctatttt aattgtaaga agtataataa taaaaccttt tgaaagtatc aatttaataa
     3961 attggaaatt tataggggct attatactaa tagtatttat tttagtatct ataatcatga
     4021 taactacact aaaaacattg aataaaattc aaaatgaaaa ttttatggaa gaaataaaga
     4081 ctttatagtg aacagcctat aaattaaaga ggttttatat gaatttaatt gcaattatcg
     4141 ataatggtgt agatattgat tttaaataat ttagaaataa aaataaaata agttatccaa
     4201 gttatatgta tgatgtaata ggggtacaat gtatagcaaa tataaatatt ggcttaataa
     4261 aactctactc ttaagtctac tgtcgtttta acacaagact aataataagt gtaacctaaa
     4321 aattattgat aatacaataa ccttgtttat gataaataac aaatattaag tgttgtaaag
     4381 atagtaagtt taaaaaaata tcaaagattt caatcacaat gtattgttta tctgtagtgt
     4441 tagctattta tggtatatat gtatttataa attgtactga atatatatat actatgctat
     4501 ctacaaaaca tttaacagtc aaaggaaatg aatataccat tataaattat tatatgagta
     4561 actttagtca gtatataata tttgctattt tacttgcatc aatgggattt gtaatacaaa
     4621 agatagtgca tatgcatttc agtgagatag aacttagtgg taactattca acaactgtta
     4681 gaaatataag tgatatctct ttagataatg ataatgaaat gtttaataga ggcaatattg
     4741 aaaatagtat tttaaaaaaa taaataatat aaatatataa aatgtgattg aataaagctt
     4801 agaatactta gtgtatttta agcttttttt attattaag
//
LOCUS       DAYAOX010000249         4763 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.252, whole genome shotgun sequence.
ACCESSION   DAYAOX010000249 DAYAOX010000000
VERSION     DAYAOX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.252"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(277..846)
                     /gene="pduL"
                     /locus_tag="ACGI57_003320"
     CDS             complement(277..846)
                     /gene="pduL"
                     /locus_tag="ACGI57_003320"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFG9718371.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(918..1526)
                     /locus_tag="ACGI57_003321"
     CDS             complement(918..1526)
                     /locus_tag="ACGI57_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9718372.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(1534..3297)
                     /locus_tag="ACGI57_003322"
     CDS             complement(1534..3297)
                     /locus_tag="ACGI57_003322"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HFG9718373.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(3340..3801)
                     /locus_tag="ACGI57_003323"
     CDS             complement(3340..3801)
                     /locus_tag="ACGI57_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718374.1"
                     /translation="MLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKIDS
                     VFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKNNI
                     DISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            3991..4452
                     /locus_tag="ACGI57_003324"
     CDS             3991..4452
                     /locus_tag="ACGI57_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718375.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKV"
     gene            complement(4545..4715)
                     /locus_tag="ACGI57_003325"
     CDS             complement(4545..4715)
                     /locus_tag="ACGI57_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718376.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
BASE COUNT         1634 a          777 c          438 g         1914 t
ORIGIN      
        1 atatataata aattaagtta gatttttcaa acttttttat ctatttgttc atattagtat
       61 aaaaagtaaa atgcttatcc ttattacatg taaattttta tatatataat tttaccatat
      121 tacatcatat ttttgtaagt ttttattaca ttttttataa taccccttta ttttataaat
      181 taatctaatc gattaaattt atcctataga aatttattac atttaaaaat atgctagcaa
      241 ataaaatatc tactagcata aagtctattt ataatattaa tcaactatta attctactaa
      301 atctccattt ttaacgcctg ctgcatttcc ctcttccata tcaacatgca tttctagatt
      361 aaagctgtca ctagctctta ctaatacatt ttcaaatact aatgctcttg gtccagatgt
      421 tttaactttt actatttgct tatcagttac accaaattta gcagcatctt cattgctcat
      481 atgtatatgt ctagaagcaa ctataactcc ttctttcatt tcaacttctc cagcaggtcc
      541 tactaattta acacctggag ttccagctat atcaccagat tcttttattg gtgcttttac
      601 tcctaatggg aaaccatctg ctaaagatat ttcaacttga gtatgagctc tagctggtcc
      661 tagtactcta actcctttta aagttccttt tggtcctact aaatccactt tttcttcaca
      721 ggcatattgc cctggttgag ataatggttt aaatttagtt aactcatgac ctgccccaaa
      781 taatacgtct atatcagctt gactcaaatg aacatgttta ttagataatg ctattggtaa
      841 tttcattgaa attccctcct tttaattatt taaattattg caatacaatt atacataata
      901 ttacctacaa aatcaaatta tattgaatca atatatatct ttaaattttt atatattata
      961 tctttaaatt cttgattatc tatataagat acatcatctg tattgtttaa atatatattg
     1021 tgtaaaagtt tagtatttat actcttaaag aaaaaatcca tcacaatctg tccaccttta
     1081 aactgtgaat catatgattt actccccccc actgcaatat acattcctat ccgtttctta
     1141 cttctatcaa ttgaaggttt atttaataca tatttacttg catatatagc ttgagttcta
     1201 tcaacaacag tctttaattt agctgatatt gaatcaaaat gtacaggact tattactata
     1261 gttccttttg aatcatcaaa cattttatat aatggcttca tatcatcatt aaacttgcaa
     1321 taccctgttt tttcacaaaa tccacatgct gtgcaatatt caatatccat attgtagata
     1381 ttaaatattt tacaacttaa tccacactct tctagcatag cttttacttc atttgctata
     1441 aaactgcaat ttttattaat tcttggactt ccatttatta ttataatctc tggactttta
     1501 agtccttttt tatcgtttga atccataatc aatctaccta tcctttaaca taactattaa
     1561 aatatcatca tcataattat cttttgatga gaagttcttt aaatccaatt ttagattttc
     1621 tactattaac tgttgattta atctaaaatt ttcttttaga aagttttcta atcggtttat
     1681 accatactct tcttttgatg aattttttat ctctaaaatt ccatcagtgt acatacatac
     1741 cattgcataa tcttccattg aaagagtatt attttcataa gtagcatctt ccattattcc
     1801 aataggtatg ccctttttac aatttaaatt attttctatt gtgccatctt tctttacaac
     1861 tacaggacta tagtgacctg cattagacac agtaatcata ttttctttag tatctataat
     1921 acctatcaaa caagttgtaa atactcccat cttgtcaaat tcatcatata acatcctatt
     1981 taaatttgtc attatttctc ttggtgtatt gtattgattg cataatactt taaaagcacc
     2041 ttttatcatt gcaacaacat aatttgatac cattccatgt cccattacat cagcaacaat
     2101 ataaactata tgttcgtcat ccacttttgt agcataataa aaatctccac ctactaccct
     2161 tgctggttta tggtaaaaat aaagttcttt ctcatcatta caatccattt tacctttatc
     2221 cataataagc tgttgttgtt tatttaatat gttcaactcg ttttcaagaa ctttgtgttt
     2281 tacttccttt tcattatgtt catataattg cataccaaca gaaacttgtt tggacaatat
     2341 actaagaata tttaaatcat cactagtata gtttttgcta tttgcacaaa ctatacatcc
     2401 tatagtatca tttttatcct gaagtttgta atatatataa ctttccaaat ttattccctt
     2461 ttttgagcat ggttttaaat tatttagata cccccccaat gactctttct ttaaaaacat
     2521 atcaatcata ggatataaat ttgttttctt tttttgctca acattataac aactaactat
     2581 agcatcctta ttatgatgac tatttataat tattaaagct tctttactat tagtaatatt
     2641 acacgcttgt ttacttaaat tttccaaaaa gccatcaata ctcttattaa ggtatagttt
     2701 tttagtagct tcatttatgg cacttattga gcctcttagt ttatcaatta aaatttgtac
     2761 attattattt ttattagtta tttctattga taaacttaag agtgatgcaa cagatgatat
     2821 aaaatctata tcttgttctg ttatataatc atcctctttt aaaaaacaag tcataaatcc
     2881 aacaactacg ttatttatta taaaaggaaa aactattctc ccaatataac cttcagcttt
     2941 tgctaaatct cgttcacctt ctgctctaga atcttcaaat acattttcca catatattat
     3001 ctttttttca tttacagcct catgtatata ctctggatat gtgtcaaaat caatttttgc
     3061 acctttaaga ctatttattg gaaaaatttg aggtacatat tctaaggttt gcgaacatac
     3121 taagtaggca tatttagaat catttttata aaatagattc acacatgcct ttgtagggtg
     3181 aacaacttct aacatttttt ctattatgtc atcttttata ttaaaaaaat ctgtagattc
     3241 agtcaccata gcggatattt ccactaaatt cttcatttta taactgccat tagtcatact
     3301 caatttctcc ccctatatat taaactattt attagtattt taacatacat tctttatagt
     3361 aaaaacaaaa acaacgacaa aactagcaac taatatccag ttaaatcctg tcaataatcc
     3421 tataagaagt aatgtaatta atgcatttag acctgttctc cctttttcat ccataaaagt
     3481 tattttagag atgtcgatat tatttttcac attatttata tgctctttca cttgttcttc
     3541 catatttctt ttatatagta tataaactac accaactaaa aacactccta tacctaaaac
     3601 catgtttaaa ttaaaagcta ccataagcaa tattgaacca aaagctaact ttaaaagctt
     3661 aaaaaataca ctatctatct ttagagttat atttctgatt tctttacatt catctcttaa
     3721 agaatatcca cttgaatcta agttattaat ctttgttttt acaatttcag aataaaaaag
     3781 ctttattatc ccatttaaca ttttcatcac tcctaaatat tttacatata tttgtaagtt
     3841 ttacaattat aatatttttt atacttggta ataataaaaa ataacaaata ttttattatt
     3901 tagtatttat tgtaaataag ttattgaagc ttgattagtt aagtatagat ttttaataaa
     3961 tatttttata atttagaagg aagtggtttt gtgcaaatag aacaactcga tttagaaacg
     4021 agaaataaaa tatactgcta tactaaaaaa attcttagaa aatatcaaaa aggcataaca
     4081 tctggaaaat taacagcaga taaatttgca gataatatat tgtctcaaga ttttatcagt
     4141 agtatactta atgaaaaaat agtcaatgag acaaacttta agatatctta tagaaactat
     4201 atagaaactt taataaatat tcaaaatgaa aatttatcta acttaagaaa aaaatctact
     4261 aaaactgcaa aatgtttcaa tatctctcaa ataacccaat taaaaaactt actaagtaat
     4321 actggttata atttattaat tccatataaa tacttaactg ctagagatat agaaggaata
     4381 gttactctta ttaatactgg gtctattgaa cttggaaatg agagaatcta caattatata
     4441 tcaaaagtat aataatttaa acctattata aaaaggcaat ttatttaata aaaaatattt
     4501 ttaatattaa ataaattgcc tttttaattt tggattaaat actgctatcc aagtgctttt
     4561 tctagtttag ataaatgttg tattggataa tattttagta gctctttaaa ttgttctact
     4621 gataaacctt ctgttgttgt atatattctt attttttcat ctaagtcact attaataaat
     4681 ttgtttttta aatcctcaaa atttacacta ctcattttac atcactcctt atgtaacata
     4741 ttcatttgta ttattttatc caa
//
LOCUS       DAYAOX010000250         4709 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.253, whole genome shotgun sequence.
ACCESSION   DAYAOX010000250 DAYAOX010000000
VERSION     DAYAOX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.253"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(112..1068)
                     /locus_tag="ACGI57_003326"
     CDS             complement(112..1068)
                     /locus_tag="ACGI57_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFG9718377.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            1436..2224
                     /locus_tag="ACGI57_003327"
     CDS             1436..2224
                     /locus_tag="ACGI57_003327"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9718378.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(2347..2817)
                     /locus_tag="ACGI57_003328"
     CDS             complement(2347..2817)
                     /locus_tag="ACGI57_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HFG9718379.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            2956..3600
                     /gene="uppS"
                     /locus_tag="ACGI57_003329"
     CDS             2956..3600
                     /gene="uppS"
                     /locus_tag="ACGI57_003329"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HFG9718380.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            3724..4548
                     /locus_tag="ACGI57_003330"
     CDS             3724..4548
                     /locus_tag="ACGI57_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9718381.1"
                     /translation="MSKGNNNNNSRNKSKKTSHLNRKKRKLNKKKLAVLICFTVLFLF
                     IAFKATQGVVALVKSMDKSNKTNQQQNVNSEQFDLGNEEENKKKKYTVFIDPGHGGND
                     KGTESKTSNRYEKDLNLQIAKKLANKLSKQKDIQVVVSRTDDTYISLKDRAILANNSS
                     ADVLVSIHLNAEKNGNTATGIETWYRNKATDGSKELAQTVQSTIVSYVKVRDRGIVEN
                     NFEVLRESNMPAILIECGFLTTPSEEQKIINEKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT         1831 a          536 c          730 g         1612 t
ORIGIN      
        1 tttttaaaag taatttatat tttaaaataa aaacacaccc cataaataca aagtaatttg
       61 catttaagag gtatgtattt atcatcaaat tattggtttg ttttggaatt tctaatcttc
      121 aaccataata tctttaaata tgaaatcatg cattgcatct agttcatatt cctcaaaagg
      181 tattacatag cctgcattac ctatttttct accattttca gtagaaagag gaaactccat
      241 agatttcaaa tttaaatttc ctatacttaa aagttctttt cctaaagata ttatttcatt
      301 aggattcata ttagttttta catacggaag tattgtgtta actagattag gatatttagt
      361 gactggtaaa tccttaacac catttattaa actctgtata acacttcttt gtcttctatc
      421 tctttcatct gtactatcat tttttcttat acgagaaaat gcaagtgtct gatatccatt
      481 taatttttgc tcaccagaat ttcttatata tttaagagaa cctttattgt catttttatt
      541 ccatttataa gtttcaggaa taaatttatt taattctctt atttcactct tatcaacagt
      601 aacagtaact ccacctaaag catcaactat atccataaaa gagaaaaagt caactacggc
      661 gtagttttgt atatcaagtt ggaagttttc ttctatagtt tctattaata agttagcttg
      721 tccatatacg taggcatgtg ttaatttttg ttctccacgt cctggtatat taacataagt
      781 atctctattt aatgaagtta gcttaagact tttattttta ttatcaatag taagtatcat
      841 catggcatca gaacgagaaa cttcttctcc tggtcttgca tctgtaccta acaataatat
      901 gtttgtgata ttttcttcgt ttttatagtc attactacta agcgcctctg tatttatact
      961 atcatcttgt atagttttta atttagtaaa aacatatcca tatatactaa caggaaatgc
     1021 tataactaaa agggctagaa atataacgaa ctttttgagt cctgacaata atatccccta
     1081 ctttctaaat tttttaatta cacatagtta attatattaa aaatttgtta cataagcaat
     1141 aaaaaacaca ataaattagc ataagtttgt cataatataa ttatttttat agcattttat
     1201 agaagaaagt gtgtagttat tattgatttt attggaatat aattgtatga agacttaaat
     1261 ttttataaac tgatgtgtat attgaaaaaa atattttcct gaatgtagtt atagcatctt
     1321 atgaaaggtg caaattgtaa tttaaaattt ttagtgtata ataagaattg aaaattcaaa
     1381 ccgataaaaa ttaataaaaa ataattataa attaataatt tgggggagga aatgtatgat
     1441 aaaattaata gcaacagatt tagatggaac tttgttagat gaaaagagcg aaataaatcc
     1501 agagttttat aaagtattta aaaaattaag agagagaggg ataatgtttt ctgctgctag
     1561 tggcaggcag tatcaaaatc ttataaaaaa atttgaagat ataaaagatg atatgatgtt
     1621 tatatcagaa aatggaacac tagtagtata taaaggaaaa gaaatattat caaatccttt
     1681 aaataaagaa ttagtaaatg aaatcataga gacaacaaga agtataaaag ggaaaaaaat
     1741 tgtaatgagt gggaaaaaat acgcttatat agaaagcaaa gatgaagcgt ttatacaaga
     1801 ggtcagtact tattatgcaa agtttaaaat tgtagaagat ttgacgaaag tagaaggaga
     1861 catattgaag atagctgtat ttgacttcaa aggtgcagaa cataataata atatctactt
     1921 tgaaaagttt tctgatagag ctcaagtatg tatttctgga gtggagtggc ttgatttaac
     1981 tgcaaaaggt gcaaataaag ggagtgccat aaaaaaagta caaaaaatgt tagatataaa
     2041 atatgaagaa actatggtat ttggtgacca gttaaatgat gtggaaatga tgaaaagtgc
     2101 ctatcatagt tatgccatgg aaaatgcaaa tgaacatttg aaacaaattg ctagatttag
     2161 agcaaaaaga aatactgaaa atggcgttgt agataagata aaagaggtta taaaaattgg
     2221 atagtcatat gtatagatga aaaagattat tttgaagaaa tcaaaaaaat atcaatgtaa
     2281 ttttgataaa taagtttatt tatgcgaaaa ttacattgat atttaataaa catcaataat
     2341 agatatttat ttttttatga tattcagtat tttgttttct atttgagttg cattttcagc
     2401 agaaattaaa taaacatatc tacctgcctt ctcaattata gtattatttg ctatttcaga
     2461 attgattttt tctccatgtc cttctccaaa aggtgcttgt atcatatctt ttttataatt
     2521 ttctagagtt ttatagattt tatctacctc gtcagttttt ataacgataa tatcttctag
     2581 agataatttt tcagaagaat taattacaaa accttgaatt atgtcttcct ctacatcatt
     2641 aaaatagtta aaatcctttg catcaaagat ttttgattcc tttataagta aatcagaaga
     2701 ttttaatgct ttttttattt ttgatgtaga aacgtttttg taatcaccac ttgaagaaca
     2761 actaaccatt cctaatgata agattatcga aaaaaacaat attaaaattt ttttcatgat
     2821 aaacctccaa gttttttaaa ctctgtctta aattagttta ctacataatt atataaaaat
     2881 aaatgtattt aatgttacag ttttgattaa taatgtaata ttttgataat aatacctatt
     2941 gagaggtgat tagaaatgag aataccaaaa catgtaggaa ttataccgga tggaaataga
     3001 aggtgggcaa gtcaaaatgg tatgacaaaa gataaaggat atgaaagtgg aatagaccct
     3061 ggactagagt cttatagatt atgtagagat ttaggctgtg aagaggtgac tttttatgga
     3121 tttacaactg ataatacaag acgtccatca gtgcagacaa aagcttttac aaaagcatgt
     3181 attgatgctg caaatatatt aatagatgaa ggtgctgctt tattagttgt aggaaataca
     3241 aaatctccaa tgtttccaga agaattatta ccatatacta atagaattaa agccaaagaa
     3301 ggtcaaataa aagcaaatct tctggtaaat tatggttggt attgggactt aaataatata
     3361 tcaggagtag aaaatgtaaa taaaaataat atacaggatt acatacagtc atttgatgta
     3421 tccagactag acttgataat cagatggggt ggaagaagga gattaagtgg tttccttcct
     3481 gtacaatcaa tttatgcaga tttttatgtt atagataatt attggccaga ttttaagagt
     3541 gacgatataa aggacgcatt tagttggtat gatacacaag atgtcacttt aggtggataa
     3601 ttaaaaatat tctgatttaa ggttgtagag tgttttaaat tattgaaaaa aaaacttctt
     3661 gatggtaaaa taacactaat aatatgtaaa agtgtaaaaa tttataccaa gaagggtgaa
     3721 gaaatgtcaa aaggcaataa taacaataat tcaagaaaca aatctaaaaa gaccagtcat
     3781 ctgaatagaa aaaagcgaaa attaaataag aaaaagttag cagtgttgat ttgctttaca
     3841 gttttatttt tatttattgc atttaaggct actcaaggtg tagtagcttt ggtaaaaagt
     3901 atggataaaa gtaataaaac taatcaacaa caaaatgtta actcagaaca atttgacctt
     3961 ggtaatgaag aggaaaataa aaagaaaaaa tatactgtat ttatagaccc aggtcatgga
     4021 ggcaatgata aaggaactga aagcaagacc agtaatagat atgaaaaaga cctaaatcta
     4081 caaattgcaa aaaaattagc aaataaatta tctaaacaaa aggatattca agtagtagta
     4141 tccagaactg atgatacata tatatcatta aaggatagag ctatcttagc aaataatagt
     4201 tctgctgatg tgcttgtttc tattcattta aatgcagaga aaaatggtaa tacagcaaca
     4261 ggtatagaaa cttggtatag gaataaagct acagatggtt caaaagaact tgcacagaca
     4321 gtacaaagta ctatagtatc ctatgttaaa gttagagata ggggaatagt tgaaaataac
     4381 tttgaagtac ttagagaatc aaatatgcct gctatattaa tagagtgtgg ttttttaaca
     4441 acaccttcag aggaacaaaa aataataaat gaaaaatatc aagaccaact tgcggaagga
     4501 attgttcaag gtgtactttc atacctagat tctaaaggga ataagtaagt taactttact
     4561 ttaatgtgag gataaattta aaagtttaac tgtgtaaatt gtattaactt tatttcgtaa
     4621 tgttgcaaac attgtttgaa tttagaaact atgaaattag aaattatttt atctatatct
     4681 aatttcatag ttttttttgc aaaaaattt
//
LOCUS       DAYAOX010000251         4677 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.254, whole genome shotgun sequence.
ACCESSION   DAYAOX010000251 DAYAOX010000000
VERSION     DAYAOX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.254"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(204..1388)
                     /locus_tag="ACGI57_003331"
     CDS             complement(204..1388)
                     /locus_tag="ACGI57_003331"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFG9718382.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="ACGI57_003332"
     CDS             1761..4112
                     /locus_tag="ACGI57_003332"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HFG9718383.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="ACGI57_003333"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="ACGI57_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFG9718384.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         1807 a          552 c          761 g         1557 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacct tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttca tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa ccatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcat ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagagcat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaatac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagct
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggtaca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaata
//
LOCUS       DAYAOX010000252         4646 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.255, whole genome shotgun sequence.
ACCESSION   DAYAOX010000252 DAYAOX010000000
VERSION     DAYAOX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.255"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(108..665)
                     /locus_tag="ACGI57_003334"
     CDS             complement(108..665)
                     /locus_tag="ACGI57_003334"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFG9718385.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(671..1747)
                     /locus_tag="ACGI57_003335"
     CDS             complement(671..1747)
                     /locus_tag="ACGI57_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFG9718386.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(1748..2431)
                     /gene="modB"
                     /locus_tag="ACGI57_003336"
     CDS             complement(1748..2431)
                     /gene="modB"
                     /locus_tag="ACGI57_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFG9718387.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(2580..3392)
                     /gene="modA"
                     /locus_tag="ACGI57_003337"
     CDS             complement(2580..3392)
                     /gene="modA"
                     /locus_tag="ACGI57_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9718388.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            3585..4514
                     /locus_tag="ACGI57_003338"
     CDS             3585..4514
                     /locus_tag="ACGI57_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFG9718389.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
BASE COUNT         1530 a          823 c          540 g         1753 t
ORIGIN      
        1 acaggcatat atttttattt taatgtatta tctatttaaa gattatatgt aaatgctctt
       61 aatttgtttg tataaatttt acttgtataa actttataca atcatattta ttcttctatt
      121 ttctttatta ctctacatgg atttccaaca gctacagtat ttggtggtat atcttttgtg
      181 actacacttc ctgcacctat tactacatta tcacctatag taattcctgg tgttatgatt
      241 actcccccac ctatccatac attatcacct atttttatag gagaaccata ctctatgcct
      301 gaatttctaa gttgtgcatc aattggatga taggctgtat agatttgaac gtttggagct
      361 aacattacat tgtcacctat ttcaatttta catacatcta aaaaaataca atcataattt
      421 gcaaagaaat tttcgcctac atgtatgtta tatccataat cacacctaat attttgttct
      481 acatttattt gcttgccaac tgaaccaaat agttgtctta aaatatcttc tcttttttca
      541 tattcatcct ctaaagtatt attaaacaat ctagttaatt ttttacaata ctctctttct
      601 tttactaaca attcatcatt tgcatagtat cctttcccag acaacatttt ttctttttca
      661 gtcatatagt ttactccttt ttgttataat tgtatctaaa gcaaaataaa tattcctgtg
      721 gaaaatctaa atatattctg tcacctttct taaatctcat tttacttttt tccaattcta
      781 tttgaatagg tacacattcg ttagataaat cagtttttct aacatatata gtatatgtaa
      841 aaggattttc tacaatgtta atcaattcga aacataactt ttcatttaaa tcttcattag
      901 aatccaagac cttaatatga tgctccctga ttcccacata ttctatatta tcacctattt
      961 ctctatttaa tgtcaactcg catccccagt ctgtagcata aatagtattt ttatccagtc
     1021 tatttaattt ggatatattt ttacaccctg ttatcttagc ttctataagg tactttggag
     1081 attcaaagag ctctttttta ggtctctttg gaagaccttc tcctttgttg tacactataa
     1141 tatcatcaca aactcggtaa gcttcttcaa tatcatgagt aacaaatagt atatctccct
     1201 tgtaatcttt taacatattc ataagttctt tttccatatt gcttcttaaa tgatggtcca
     1261 atgctgagaa aggttcatct aaaagtaata tatctggaga tgttgccaat gctctagcta
     1321 gtgctaccct ttgttgttga ccaccagata attgccaagg gtatctcccc tcaaacccat
     1381 ttaatttaag tatatctaag taccttgcta ctatttcctt tttttcactc ttacttagtt
     1441 ttaaaagccc aagttctata ttttgactta cagtcatatg tggaaacaaa gcatagtttt
     1501 gaaataaaaa tcctaccttt ctatcttgtg ttgataaatt tatcttcttt tcagaatcaa
     1561 atagaactct atcatttaat acaattttac ctcttgttgg tttctcaagc cctgctatac
     1621 attttaatgt catacttttt ccagaaccag attctcccaa aaaccccaga gtaccttttt
     1681 cttgtttaat ttctaccttt agtttaaaag aggataaatc tttttcaata tccacgtata
     1741 agctcaacta tccacacctc tttcctataa ttttctgctg aaattccgac caataattta
     1801 gtaataaaat cattgctata gatattgcta caataatcat aacccacatc atagctttat
     1861 tcatatctcc gccttcaaca gcaaaaaata tagctattgg catagtttga gtttttccag
     1921 gtatatttcc agctatcata agtgtagcac caaattctcc aagagctctg gcaaacgaca
     1981 gcaccaatcc accaattatt ccaggccatg ctaatggaac tgcaatctta aaaaatactt
     2041 tccattcatt aagacctaat gttctagcag ctgacaacat attattatca atctgttcaa
     2101 aagctgacct tgaagttcta tacatcatag gaaaagacac cactactgct gaaattacag
     2161 ttgctgtcca actaaatatt aaagtcctat cgaaatttaa taaaaacttt cctataaatc
     2221 cattcttccc acatagtaat aataaaaaga aacctacaac tgttggtggc aaaattagtg
     2281 gaagtgtaaa tagtccatct ataagaccct tccacttacc tctatagtta gccataatat
     2341 atgacacaaa tattcctatt acaaaagtta ttattgttga caaaatagat gtcttaagtg
     2401 atatccataa gggtgaccaa tctgttccca tctttccctc cttaatttta aatggaaata
     2461 gtgaaactaa taactctatt aatttcacta tttccatttt attataatac acaattattt
     2521 tcaattttca tttatttata tataatttat acttaattac acataattta tttctaagtt
     2581 tattctactt ttttatatcc aaattcttca aaaatctttt gtcctgactc acttaataaa
     2641 aattcttcaa atttcttagc agcatctacg tttttactag ctttaattac tgaaactgga
     2701 tatgtaatag gcgaatgagt tttttcgtct gttttttcaa ctacttttat cttatcatta
     2761 ttgacagtat cactgaagta aacgaatcca acatcagcat ttcctgattg cacccatgct
     2821 aaaacttctt ttacatcttt tgcaaatact agcttatctt ttaacttatc ttttaaattt
     2881 aaattagtta acacctcatc tgcatactta ccagctggaa cactttcagc ttcaccaaca
     2941 gcaattttct tcactttatc tgtagtcaaa tctttcatac ctgatacact actgtcagca
     3001 gaagaaataa gaactaaatc attttttact aaatctttca tagtatcaga aactaataac
     3061 ttctcttcat ccaaagcttt catttgtttt tgacctgctg aaataaataa atcacaaggt
     3121 gctccttgtt ctatttgttg ttgtaatgag cctgatgctc catagttaac tgtaagtttt
     3181 acatttgaat caactttttt atattcttct tcaatctttg ccattgcttc ttttaaactt
     3241 gcagcagccg atatatttaa ctctactgaa tcactacttt tagttgtttt ttcttgtttt
     3301 ccatcatctt ttttgtcttc attactattg cacccaactg tccccaaagt taaacatgca
     3361 actaatccta atattcccaa tatttttttc ataacaatct ccctttttgt aaatttgata
     3421 cacattttaa catgttttat tatgttttat catgttttgt tatatatagt ttaattatat
     3481 atacatgata ataaattttc aagtaattta tttacactac ttttaaatat tttgctacat
     3541 tttccttttt ttaatgttaa aataacctta tatggaggtg gattatggac tcattatcat
     3601 ctttgacacc tgtcgaggta gctgaattat taaagataac aaaaaatact gtatatgaat
     3661 taatcaaaag aggtgaactt ccttcttata aagtagggaa aaaaataaga gttgatacta
     3721 aagatgttga agaatatatc aacagccaaa aaactggtaa agtaaaggat acacatttca
     3781 acaataaaaa tatagatata aataataatt tcaaaattat tatttctggt caagatgtaa
     3841 tattagacat tttagctagg tctgttgaaa aaaaattgga tggaataagt acatttcgct
     3901 cttatatagg tagttataat gctttgtatg agttatataa tggcagagtt tctatagcat
     3961 catcccatct atgggatttc gaaacggatg aatataatag cacttttgtt aaaaagctat
     4021 tgccaggaat accatgtgta cttataaatc tagcttatag aatgcaaggt ttctatgttg
     4081 ctaaaggaaa tcctaaagag ataacaacct gggaggattt aacaaaacca agtataacca
     4141 ttgtaaatag ggaaaaaggt tctggaacta gggttttact agatggtaaa cttagacttc
     4201 taaactttaa tggtaaacat ataaaaggtt atgataatga agaattatca catcttggag
     4261 ttgcaagtac tgtttctcgt ggaatagcag atgttggttt gggaaatgaa aaagctgctt
     4321 tacaagtaaa taatatcgac tttattcctc ttcacaaaga gcgctatgat ttagttatta
     4381 aaaaagaaga cttacaaaat ccagtttacc aaactataat taacataata aactctcctg
     4441 agtttaaagc tgaacttcaa ggtcttggcg gatatgattt aactgatact ggaaaaacta
     4501 tagcaaaagt ttaagattaa aatcgcaatc tattttataa gatttataat ttttcacaaa
     4561 atttataact taaacacgaa ataaattgcg attttagtgt ctcaaaaaat ttatgtcttt
     4621 cattttatca tccaatacaa aatata
//
LOCUS       DAYAOX010000253         4598 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.256, whole genome shotgun sequence.
ACCESSION   DAYAOX010000253 DAYAOX010000000
VERSION     DAYAOX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.256"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            297..986
                     /locus_tag="ACGI57_003339"
     CDS             297..986
                     /locus_tag="ACGI57_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9718390.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            986..1903
                     /locus_tag="ACGI57_003340"
     CDS             986..1903
                     /locus_tag="ACGI57_003340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9718391.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAMLQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            1976..2902
                     /locus_tag="ACGI57_003341"
     CDS             1976..2902
                     /locus_tag="ACGI57_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718392.1"
                     /translation="MNYIIETNQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            2899..3579
                     /locus_tag="ACGI57_003342"
     CDS             2899..3579
                     /locus_tag="ACGI57_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFG9718393.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            3680..4012
                     /locus_tag="ACGI57_003343"
     CDS             3680..4012
                     /locus_tag="ACGI57_003343"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFG9718394.1"
                     /translation="MAQPFEVEPKLDELIEWYYSQSEVSIKVITEFHYRFELIHPFQD
                     GNGHIGRFVMLKQMLENNLPIKIVSWDSEELYRNSLNSCSLGNYVPLIEYLSSLEDFR
                     EVYKMLWK"
BASE COUNT         1748 a          480 c          737 g         1633 t
ORIGIN      
        1 agaatgtggt ttctttttta tgtataaaat attttttagc aagagttaaa aggtgtttta
       61 taatataaaa tagttaattg tagatgttaa taaaagaaaa ttatgtatat ttcagataaa
      121 gataagagag tatactatat tttaattatt ttttcaataa agcttattga aaagaacttt
      181 aacttagaag agtatcacca aattgcagat acaatatcat aagcagttat ttattgacaa
      241 ccctttttaa taaatatatc atttgaagta tactgacgaa aaggagttga ataataatgg
      301 acatcttatt ggttgaagat gataagaata ttcgagaaat ggtatacgat tttctgatta
      361 gtgagaatta ctctgttaaa actgccattg atggaaatac agctattgaa atatttaaaa
      421 attattcatt tgatattatt ttactggact tgatgttacc aaagtctagt ggttttgaag
      481 tattaaaata tatacgaagt gtgagtgttg ttcctgttat tattactact gcgaaagata
      541 gtgacacaga taaaatgcta gggcttaatc ttggtgcaga tgattatatt acaaagcctt
      601 tttcaatcgt agaattgctt gcaagaatta aagctaatat tagacgtgca acagtgtaca
      661 gcagttcaag aaactctgaa gatatggtta ttcagtataa agatttgatt ataaatatat
      721 caaaatatgt agtttcaaag aatggaattg acttaaactt aacacataca gaatttgaaa
      781 ttgttaaatt actagcgact aatcaaggac gtgctttttc aaaggaacaa ttatacaacg
      841 ctatttggaa agaaccttat tatggaaatg aaaatgtgct aaatactcat attaacaggt
      901 taagaaataa attgtctgat ggtatagaaa aaaataatta tattaaaact ctttggggaa
      961 ttggctacaa aatggaggaa gaataatgga gttattttta gttgtaattt cgattgtact
     1021 atttgctctc tacttaaaag aacgaaataa atcaaagaaa atggaaagtg attataagta
     1081 tatcaattca agacttcgtg acataacaaa tgatgatgaa attaattata ttttagtacc
     1141 atctgatatt tcgattgtaa aagaaacagc agtagatata aataaacttc ttgaaaagtt
     1201 ttacagcagg caaattaatt ataatagaag tgaaaaagcg atgttgcaaa tatttacaaa
     1261 catatcacac gatttacgaa ctcccatcac agtattaaag ggttatattg aaatgttata
     1321 tcttcaaagt caaaaagaag aattatcttc ttcaatgaga gcaactgtgg aaaaaatgca
     1381 aacaaattca aatgagcttg ttaattcaat aaataacctg tttaatatgg caaaaatcca
     1441 atctggagat atgatattaa atcttcaaaa atccaatcta acacaattat gtcatgagat
     1501 tattttagag ttttatgatt tgctagataa agaaaacttt aatgttgaag ttaatattga
     1561 agatagacct atatatgcaa atgttgacac agaagcatta caacgtatac tcaaaaattt
     1621 aattgataat gctataaaat atggttcaga tggtagattt ttagggattt cactatatga
     1681 aaaggaaaaa catgttttta tagatattga ggataaaggt tcaggtattt ctgaaactga
     1741 aaaagagcat attttcacaa gagcttatac tgctgacaga aaaaaaggca atggtttagg
     1801 tttggctatt tctcaagggc ttgtaaactc tatgggaggt tcaatttatg taaatagtag
     1861 acctaatgta aaaacagttt ttacagtaaa attgaaaagt tagaaaaaag ttagatttaa
     1921 aagagagaaa agataatttt attttgtata ctgtttttag aaaggaggga gtatgatgaa
     1981 ctatatcata gagaccaatc aattaaagaa aatatataaa gataaagctg ttgttaatgc
     2041 tgttaatatt catgtgaaaa agggagaaat ttatggattt gttggaccaa acggggcagg
     2101 taaaagtaca gtaatgaaaa tgttgctaaa tcttgttaag ccaaattctg gagaaatagt
     2161 tatgttcggt aagaaagtgg cagaaactga ttttgaaata ttaaaaaaga ttggtactat
     2221 tattgaaaat ccgtatttat atgagaattt gactgctaaa cagaatcttg atttacactg
     2281 tgaatatatg ggttattata acaaagaaca tatatcagag gtattggaat gtgtcggatt
     2341 gtcaaagcaa tcaaacaaga aagtttcaaa atattcttta gggatgaaac aacgtcttgc
     2401 aatagctaga gcaattctta ctaaaccaga attattgatt cttgatgaac ctataaatgc
     2461 actcgaccca gaggggataa gagaaatgcg agaattattc cgtaagttaa atacagatta
     2521 tggaataact atatttattt ctagtcatat tctttcagag gtagaacaaa ttgcggacac
     2581 tattggaatt attcaagatg gtaaacttat taaagaaatt tctatgtcag atatacacaa
     2641 atatcaaaca gattatgttg aagtagatgt agataatgtg gagcttgctg gatacttgtt
     2701 agaaaaagaa tttggaataa aagactttaa aattacttcg gaaagttgta ttgaaatata
     2761 tgatttacga aaagatgtaa aagaaatatc caaaatattt attcaaaatc aaattggtat
     2821 aaatagcatt ggacgtaaac aaagttcttt agaagattac ttcttccaaa ctacaggtac
     2881 aggaggtaaa gaaacactat gatacattta atgaaactag agctaaaaaa agttgggcta
     2941 aaaagatatt ttttattttc aatgctgggg attttcttat gtatgttttt tgtctttact
     3001 ggattgaatg ataaatctac ttcaatatat gattatgatg ttacatttag gacaatcagt
     3061 ttatttttct gtttttacta tattatatta tttgctgtgt tgaatgtggc atatataata
     3121 aatgaatata caaataaaac aatcttgatt ttattctctt atccacttga ccgtaaaaaa
     3181 cttattttat caaagttact tttgattacg actcttatga tatcatcaat gatagttgga
     3241 tatatctgtt gttttacatt cgttgttgtg ttggataagt attttaattt agttattgga
     3301 gattttcata tttcaatttt atcttattgg ataccaggag caattaaaag tattattgta
     3361 ttttgctcat taggtatatg gacattcgtt gttggaatga taagaaaatc aatacctgtt
     3421 acccttgtca gttcaattat ttttatcaat ttaagacaat ttattttagc tggtacaaat
     3481 acaactcaag acagtttgat ttctgttttt attatagttg gtataactgt gattggtata
     3541 tattatatac taaatcataa agttactgaa attgactaga tactgtggat aggaaggtag
     3601 ttattaattg tatcttctcg ggataataat gatttgctat tatatcagct attattaata
     3661 ttattagagt tttaataata ttgcacaacc atttgaagtt gagccaaagc ttgatgaatt
     3721 aattgaatgg tactatagtc agagtgaagt atctattaaa gttataactg aatttcatta
     3781 tagatttgaa ttgatacatc catttcaaga tggtaatggt cacataggta gatttgttat
     3841 gctaaaacag atgttagaaa ataatcttcc tataaaaatt gtatcttggg atagtgagga
     3901 attgtataga aactccttaa attcttgtag tttaggtaat tatgtccctt taattgaata
     3961 tttatcaagt ttagaagatt ttagagaagt atataaaatg ttatggaaat aattgtttgc
     4021 agcaaaaaaa gtttatatat aaatgtattt ttatctatgc tatttaacgc taatttctta
     4081 gttattctta gcttggacta tacccactat tttaactata taaaatagtg gatatagttt
     4141 aactatctct ttataactat ttttatgtat cttagttgtt tcttaatatg gggacagttc
     4201 ataaaactaa aagtgatttt taattttatc atttataatt taccattaca tcagaaaaat
     4261 gcttatcatt agaattttca taaaaggtac ctattttttt caattaactg aatcaattct
     4321 attagtctta atctttcata tttcaaattg ataatataag aaaataaaaa ataaaaaatt
     4381 ttaaattttt tatttattgc gaaaaaaata aaaaggtgct atactttttg taacatatta
     4441 ttcaaattat taagagatag taaacattta tagtttaatt tgtagtacac tagcatgatt
     4501 tgcagaacaa tgaattaatc tatagtataa tagtttaatt taaagtacaa cgatttaatc
     4561 tatagtacaa taacttgatt tgaagtacaa cgatttaa
//
LOCUS       DAYAOX010000254         4561 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.257, whole genome shotgun sequence.
ACCESSION   DAYAOX010000254 DAYAOX010000000
VERSION     DAYAOX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.257"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            43..978
                     /locus_tag="ACGI57_003344"
     CDS             43..978
                     /locus_tag="ACGI57_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9718395.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            1014..2387
                     /locus_tag="ACGI57_003345"
     CDS             1014..2387
                     /locus_tag="ACGI57_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9718396.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(2575..3342)
                     /locus_tag="ACGI57_003346"
     CDS             complement(2575..3342)
                     /locus_tag="ACGI57_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HFG9718397.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDFI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(3372..4481)
                     /locus_tag="ACGI57_003347"
     CDS             complement(3372..4481)
                     /locus_tag="ACGI57_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718398.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRNLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT         1569 a          553 c          708 g         1731 t
ORIGIN      
        1 atagataaaa ttaaatgatt caaaagagaa aggtgaaaag agatgtatta tataggtgta
       61 gacataggag gaacaggtat acaagcagga gtagttgata actatgggaa aataattttt
      121 aggtcagagt gtaaaacagt aattgaaaaa ggctttgaag gaatacttaa tgacataaaa
      181 ataatgattt ataagttgtt ggaagataat aaacttacta tgagtgacat aaaatctatt
      241 ggatttggtg ttccaggttt tataaataaa gaaggattag taacttgtgt aaatttaaaa
      301 tggaataaaa aagcttttaa taaagagtta aaaagaagat ttccagatgt tgaaattcat
      361 ggagaaaatg atgcaacagt agcagcactt ggagaagcaa agtttggaag tatgaaaggt
      421 gctaatgttg gagtattata tactttagga actggagtag gtggaggaat agtaattaat
      481 caaaaagtct tttctggagc acatggattg ggttcagaaa taggtcatca aattatagga
      541 gaaaactatt ttaattgtaa ttgtggaaat aatggttgtg tagaaacttt ttgttcagca
      601 actgcaatta taaagtattc tcaaaaactg atagaagaag gagaaaagag taggatttta
      661 gatttggcag agggaaattt agaaaatgta aatgcaaaga tggtgtttga tgcatataga
      721 gaaaatgatt tagtggcaat taaagtaata aatagattta aagagtattt agcaaaaact
      781 tttgcaaaca caataaattc tttagaccca gaaattatat ctataggagg aggcatttct
      841 aaatctagcg atataatact tgatggaata gaagatttgg taagaaaatt tgtattatac
      901 aaaacagaag atatagctac aattacatgt gcaactttag gttcagacgc aggaattata
      961 ggagcagctt ttttataatg taaatgaact gcttattgga ggcgtgatga tatatgggta
     1021 aaacaaatgc tagaagaacg ctttttttga tagcaatagg ttcaggaact atgctgaatc
     1081 ctcttaattc ttctatgatt tcacttgctt tacatagtat acaaaatgat tttcatatta
     1141 gttttacaac tgtatcttgg attgtttcag cattttacct agctagtgct attgcacaac
     1201 cagtttcagg taagatagga gatttaatag gtagaaaggt cttatttttg agtggtttga
     1261 tgcttgtaat tttatcagca tccatagtac cacttgttca atcattcttt atattgattt
     1321 ttataagagt aattcaagca attggaacaa gtaccctata tccatctgga gttgctctta
     1381 tacaaaataa tataaaagaa agacaatctt ctgcattagc agttctaacc atttttgctt
     1441 caactacagc aggattaggt ccgacacttg gaggattatt gttggactta ggaggatggc
     1501 atgctatatt tcttgtaaat atacctgttg ttttggtttc tatatgctta gggtattttt
     1561 tattcccaaa agaagtaaaa gaaaagaaat ctataaaaga aactttaaaa aatgttttgt
     1621 caagattaga tattattggc atacttctat tttcaattgc aatgattttt atattactat
     1681 ttttgctatc tatgaaggag tcttttaact tggaacaact tgtatttggt ataatactga
     1741 tgtgtctttt tatttggcat gaattgagaa caaaattgcc atttatagat attaagcttt
     1801 ttgtaagtaa tcctaaattg tcaaaagttt acttgcaatt tattatatta aatttcttta
     1861 attatatttt gttttttggt cttcctagtt attttcaaga tgccttacat tatagtgcaa
     1921 agtcgactgg attatttatg ctatttatgt ctggtatagg tatatttata tctccattaa
     1981 ctggaaaatg gatagataaa tctggaacta gatttcctct tgttacatca tcaatcttta
     2041 tgtttattag tgcagtactt ctttcagtag tttttatcca tatacctgta ataggaaagg
     2101 gtattatatt atcattagca ggtattagtt atggagttgg taatgtagcc ctgcaatcct
     2161 ctatgtttga agaaagtcct cgtgacagta tagggacagc ttctggtctg tttcaaacta
     2221 gtcgttattt aggttctatt ttagcaactg ttttacttgg aatggtattt gaagtaaata
     2281 taacatcaga acaatttcag atattaggtt atgttatggt ggtcttaggt acaataagtt
     2341 ttttattaaa ttttatatta aagaaagaat taagaaaagt agaatagtgg atttatggat
     2401 ttaataagtc taataactga taagtttaaa aatattgttt gtaaaacata actggtttga
     2461 tatttattaa ttctaagttt tgaagcatag agtactcgat aagtatacaa tattttgaat
     2521 taaaattaag cagaaatttg aactagtctt tcaaatctct gcttaattta gtagttaaat
     2581 ttttttatag agaaaataag atttgacact tagataaaaa atcattgcat tttcaaaatt
     2641 ctttaaatca aacccaagta tctcattaat cttttccaat ctatatacta gcgtgtttct
     2701 atgtaaaaac atactagtag atgtcttaga aatatttaaa tcattattta taaattttat
     2761 tcctgtatat agaagttctt cgtcaataga tttaatctta ttaaaatcta ttaatttatt
     2821 gagtatatta tcctcattaa ttaaatctaa agttttatat ctaataaagt caaacaaatt
     2881 taaaaactta ttttcagcgt tgtcttttat aatatgaggt agtaaatcag aacataactg
     2941 taatctaaaa tcaaaatcag ctttactatc taattcacca agataaaaaa tataagtact
     3001 ttgataagtt tcagtttcaa ttatattttg tgtagttaca ttaatatcta agttatattt
     3061 agaatttact ataaaaaaac cattgtgttg tattatattt atatcttcaa ataaattata
     3121 aaaaatattt tttagatatt caaagtctat atgagattgg taaattaaat aaaagttgcc
     3181 ttttaaatta tcgagattat ttttatctag aaaatcaata acataaatag cattattgaa
     3241 agtaactaaa tggctactag catctaactt ttggttttca tatggataga tatttatttt
     3301 tttattaagc tgtctttcta aaaaatcttt taactcttcc ataaagtctc cttaaaaata
     3361 agattatatt cttatcttat agctaaagta gtttctttgt caaatatgtg tactctacta
     3421 ttgtcaactg aaagtgttaa gtcatcacca acattaattc tgtttttact gtcaaatcta
     3481 attgttatcg tattttcgcc tagttttaag tgagcataag tttctgctcc cataagttct
     3541 tttatttcaa ttcttgattt aaaacaagta tctggattag atttaataaa agtatcttct
     3601 acatgaatat cttctggtct gatacctata acaacttcat tattaatata attgtctttt
     3661 attaaatcat agtgtttttc tttattcaat ttaagcttaa tatgttcatt ttgtgcataa
     3721 atttcatcat tttcttctat taatattaca tcaataaaat tcatctgtgg tgctccaata
     3781 aatcctgcaa caaatatatt agctggataa tcgtatacat cttgtggagt tgcaatctgc
     3841 tgaactactc catccttcat tactacgatt ctatcagcca tagtcatagc ttcaacttgg
     3901 tcgtgtgtta catagataaa tgtagttcca agctgttggt gtagcttagt tatctctgtt
     3961 ctcatagcag ttctaagttt tgcatctaag ttggatagag gttcatccat taagaatacc
     4021 ttaggatttc taactattgc acgtcctaag gcaacccttt gtctttgtcc tccagataat
     4081 gttttaggtt ttttatttaa aagaggtagt aagtctaata ttttagcagc ttctttaact
     4141 tttttgtcta tttcatcttt aggcaaattt ctaagtttaa gtgcaaatgc catgttttcg
     4201 taaacagata agtggggata taaagcatag ttttgaaaaa ccatagctat atctctatct
     4261 tttggttcta tattattgac aagtttatct ccaatgtata gttctccttc agagatttct
     4321 tcaagaccag caatcattct aagtgtagtc gatttaccac aaccagatgg tcctactaaa
     4381 acgataaatt ctttatcttt aatgtctata tttatatttt ttactgcgtt aaatccattt
     4441 gagtaaagtt ttgatatatt ttttaaaatt acttctgaca taatatcctc cttgatattt
     4501 aaattttaat tatatagtat caaatttttt tattatatta aatgtgtaaa atgactaaat
     4561 g
//
LOCUS       DAYAOX010000255         4558 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.258, whole genome shotgun sequence.
ACCESSION   DAYAOX010000255 DAYAOX010000000
VERSION     DAYAOX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.258"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(11..724)
                     /locus_tag="ACGI57_003348"
     CDS             complement(11..724)
                     /locus_tag="ACGI57_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HFG9718399.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1016..2680
                     /locus_tag="ACGI57_003349"
     CDS             1016..2680
                     /locus_tag="ACGI57_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFG9718400.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLKQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(2787..3686)
                     /locus_tag="ACGI57_003350"
     CDS             complement(2787..3686)
                     /locus_tag="ACGI57_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HFG9718401.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(3713..4351)
                     /locus_tag="ACGI57_003351"
     CDS             complement(3713..4351)
                     /locus_tag="ACGI57_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HFG9718402.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
BASE COUNT         1564 a          706 c          554 g         1734 t
ORIGIN      
        1 ttctatattt ttaaccaaca gatgttttct tcaaattaat cagatgattt agtatatctt
       61 gaacctttcc agagtccttt aaatatccta atgccccatt tatttgtgat tttaaatcta
      121 tttctacttc catatccaaa tcaacatttc ctttttttat agatatattt ttaagtgtta
      181 ttgggtaaag ataacttttt tccaaagtaa tcttatttcc attaacctca aatggaactt
      241 ttttattgta gttacttaat attttttcta gcattttatc acttagttta aattttccaa
      301 ttttcacatt ctcaagactt acaactaagt ttccatcctt taaagtaggt ctcaattcaa
      361 gttcatattg actatttacc aaaccaaata ccttatatgg tcctgctacc tttattttac
      421 catttttcat ttcaacaaaa ttattttcaa gttcattaat tccatgcttc tgcatcaatg
      481 tatatattaa atttttaaac tcttcctctg aaacagatat ttttccaacc attctaagtg
      541 gattttttac tacctccata gaatttatat atgagccttt tgataaatat tcttcatcta
      601 tgattttatt attttgtgaa ctaacatcat atctttcttt tggtgtcaag atatatacaa
      661 caattcctgc aagtactaaa acaataacaa ttaaacttaa caaaatcttt gcaacctttc
      721 ccataatcgt ctcctttaca aatctgtcat cagatttaaa ataaatttag ataactaact
      781 tcacataata ttcttaacaa tatacatact ataatacagt cttttaaaaa ttaattcaac
      841 caatctaatg ttttgtaaat aaactgtaaa aacatgtttt tagtataaaa gttaatattt
      901 tgcataatac tcttaattac acctttattt ttttctaatc ttggattaaa attaatatta
      961 tgcaatacag tgtagtgttc tgtattataa tcaaattaaa taggaggtat agaatatgtc
     1021 aaatgaaacg aactcatcta actttataaa gaatattatt ataaacgact tagaaacagg
     1081 aaaacatgac agtatcataa ctcgttttcc acctgagcca aacggatatt tacatattgg
     1141 tcatgcaaaa agtatatgtc ttaattttgg attagctaag gagtttaatg gaaaagctaa
     1201 tttaagattt gatgatacaa acccattaaa agaggatgtt gaatatgtag agtccataaa
     1261 agaagatgta aagtggttag gatttgattg gaacgagtta aattttgcat ctaactactt
     1321 tgatgaaatg tataagagag ctttaatact aataaaaaag ggtaaagctt atgtatgtga
     1381 tttgacacaa gaggaaatga gagaatatcg tggtactcta actgaaccag gaaaagaaag
     1441 tcctcataga aacagaacta ttgaagaaaa ccttgattta tttgaaagaa tgaaaaatgg
     1501 tgaatttaaa gatggtgaaa aaacattaag agccaaaata gatatgtctt ctccaaatat
     1561 aaaccttaga gacccaatta tatatagaat atctcattca acacaccata atacaggaga
     1621 taaatggtgc atatatccta tgtatgcttt tgctcatcca attgaagatg caattgaagg
     1681 aataactcac tctatatgta ctttagagtt tgaagaccaa agacctcttt atgattggtt
     1741 tgttaaagaa tgtgagatgg aaaatatacc tagacaaata gaatttgcta gacttaatat
     1801 aaataatact gtcatgagta aaaggaaatt aaaacaactt gtagatgagg gtattgttga
     1861 tggttgggat gaccctcgtg ttcctacaat atccggaatc agacgtaagg gatatactgc
     1921 agaagcattg cgcaatttct gtagtgaaat aggggtatca aaagttaact ctacggtaga
     1981 tagtcaaatg cttgattatt tcctaagaga aaacttacaa ccaaaggctc ctcttactat
     2041 gggagtttta agacctttaa aacttattat aactaattac ccagaagata agattgagat
     2101 gcttgaaata gagaataatg ctaaagatga atctcaagga aagagattag ttcctttctc
     2161 tagagaattg tatatagaac aagatgattt tatggaagaa cctgttaaga aatattttag
     2221 atttttccca ggaaatgaag tccgtttaaa aggtgcttat tttgtaaaat gtactgatgt
     2281 aattaaggat gaaaatggaa atgtagttga aatacatggt acttatgacc ctgaaactaa
     2341 gagtggctct ggctttacag gacgtaaggt aaaatctaca atacattggg ttgatgctaa
     2401 atcagctata ccatgtgaat ttagattatt tgaaccatta attcttgatg atatacctga
     2461 aaatgaaggt aaacatttct taaaacaaat aaatcctaat tcattggaaa tattacaagg
     2521 ttttgttgaa cctacacaga taaaagatgc taaacctttt gataaattcc aatttgttag
     2581 aaatggattc tttagtatag ataataaata tacaactgat gaaaaatttg tatttaatag
     2641 aatagttcct ctaaaaagtt cttttaaacc aggtaaataa tcttacaatc aaaaaatgaa
     2701 gacttaaaat ataaattact tctactataa ataaacttag ttagaagtaa ttttttattt
     2761 gagtcttcat catttacttt aaatgattaa cttttttcac tacagattcc catgacttct
     2821 attccatctt ttatgcattc tatgttaaga ggaaaatctg gtcctttttc tccgtcaata
     2881 tctgttataa gtgaatcttc acattctata tgcacctttt tagttttaaa gtatagtatt
     2941 tcatttccat tatattgatc taaataatca cctctaagca cacttatcaa aactggtatt
     3001 gattttggta ttggcattcc tttaaaaata attacatcca aatatccatc atctatttca
     3061 gccttatatg ctagatttat atttcctgct gttttgccat taaaaatcaa cataagatac
     3121 atatctccat cataatacat ttcctcagaa gatactttta tcttaaattt cctcatatac
     3181 aatgcttctt ctattccctt tatataataa gaaattcttc caaatgaatt ttttaagtct
     3241 gtgtttatcc tttgtgatac atccgtaaac ataccagcac ttgcaacatt tataaagtac
     3301 ttattattga ctttccctat gtctattttc tttggttttg aatttatgat ttcatttata
     3361 gcttctttta tatcaaatga aatttgaaga gcatttgaaa aatcatttgc agttcctgta
     3421 ggcaaaattc caattggaat ttttatgtct aattctttca ttgcatttac tactaaatct
     3481 acagtaccat caccaccagc tatcaatatg tgttcataag aactatcaac ttccttaaat
     3541 gcattcttta caggttcttt aatagttaat ctatagggta tcaataaata atttttttct
     3601 tgatataatt ctatgatagt gtctaaattt gataatatct ttttttcacc tgaattagga
     3661 ttgtatataa gttttacttt tttcattatt tctccttact cattttattc ttttagtatc
     3721 caaaatcttc atttaaaaaa atcacatgta ttctttctgg tgttaattgt ctttttataa
     3781 gtgtataact acagcatatt cttcctggac ttgaacagtc tatacatata ccagtagtct
     3841 tacatccagt atttgtactt aatcttttac aattggctgg agcagctatt gtttttgttc
     3901 tttttatagc ttcatctaca tctttaacta ttttatttac tcctgctata actattactt
     3961 tatcaggtcc atatatcata gcagcaactc tattcccatt cccatctaaa ttgtaaagtt
     4021 ctccatcctc tgttatcgca ttagtacttg taaaataagc atctgcagaa aatgactttc
     4081 tatatatttc tttcatttct tctggtttga tatcttttgc atatctatca agaaaattat
     4141 atttttctct tataaaatca atcactcctg tttcaaataa ggtcatagaa cctccacatc
     4201 ctactagaga acctgcttta actatttctt caattttttt aagaagctct tctttgttac
     4261 ttactaaata ccctttcata ttattgtttt ccaaagattt tattgttttt tctattcttt
     4321 tttcattaag ccaatttaaa ttttgatcca ttttaacttc ccccttattt ttaatatgca
     4381 cttaattatt tataaacttt attttcttta taagtttatt ttagtacatc taaaaaaaaa
     4441 tacaatataa aacttaaata gttttacata attttttcca aaataactta ctcaatcaaa
     4501 aaaggaaagt agatgtacta ttctactttc cctatattgt atgtagtatt tattgcct
//
LOCUS       DAYAOX010000256         4554 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.259, whole genome shotgun sequence.
ACCESSION   DAYAOX010000256 DAYAOX010000000
VERSION     DAYAOX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.259"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(86..880)
                     /locus_tag="ACGI57_003352"
     CDS             complement(86..880)
                     /locus_tag="ACGI57_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718403.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(881..1756)
                     /locus_tag="ACGI57_003353"
     CDS             complement(881..1756)
                     /locus_tag="ACGI57_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9718404.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLSFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(1760..2128)
                     /locus_tag="ACGI57_003354"
     CDS             complement(1760..2128)
                     /locus_tag="ACGI57_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9718405.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(2311..2970)
                     /gene="deoC"
                     /locus_tag="ACGI57_003355"
     CDS             complement(2311..2970)
                     /gene="deoC"
                     /locus_tag="ACGI57_003355"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFG9718406.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(3006..4193)
                     /gene="murJ"
                     /locus_tag="ACGI57_003356"
     CDS             complement(3006..4193)
                     /gene="murJ"
                     /locus_tag="ACGI57_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFG9718407.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(4270..>4554)
                     /locus_tag="ACGI57_003357"
     CDS             complement(4270..>4554)
                     /locus_tag="ACGI57_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFG9718408.1"
                     /translation="NRKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQIC
                     NKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         1524 a          777 c          451 g         1802 t
ORIGIN      
        1 cttaaaatca acatttatta aaactaataa acctgcttta aaatacaaat ttacaaattt
       61 gatttttaag caggttttat tttagttata ataagtcatt tgcatactct ttaattggtg
      121 cttttctaag taataaaatt ataaatatta tgcaaataac tataattcct acttttgttc
      181 caaataaaat aaaaggattc tgaaatgaat aaataatttt ttctaaatac aatagcattt
      241 tatgcatatt tttagttgca aaattaacta tataaattat cattaatatc ggtaatccta
      301 tcaatatagt aatactagta acttttctaa ctctatatgt aaaagctcca agtaacactc
      361 ctataaaacc tatacatatt tcatttatac atatttgtac aaaataattt aaataagtta
      421 gtggcatact tgatgttgaa ggatctatca gtgctgatat agatgtccaa atcatatctc
      481 tctttagtaa aactgatagt tctatactgt aacctgttat aatttctata aataacttcg
      541 ataaaaaaat caaaactata cctaaaatag aaatcacaaa gcttaaaata attaaatcta
      601 tgacaatagc ttttatacaa ctttttctat ctgctcttat acttaaagct cctgaaaagt
      661 ctttatttgc tatatttact ccatacacaa atagcagtat agttatatat gttaaaatag
      721 gattcacaat cgtggataaa ttagttttag atgcttctcc aaaaatcttg agaataaaca
      781 ctggcaaaat attcataaca attcccacaa tcataaatat aattatatac aatttagtat
      841 ttttattaaa aaattttata tatggcttta attcattcaa ttacaattcc tccttcttag
      901 tcatgttaac aaacatatct tgtagcccta tgtatcctac atcaatattt gaatttttaa
      961 gtattttttc atcactttca ctaaaatcac catagtaaaa ataagcaact gcatttccaa
     1021 atgccttttt aggttttata ttttttatac attctaattt ttctaactct tctttatttc
     1081 cagtaatata atgagctttt tgttttatga catcaatttc ttcatctata ataatttttc
     1141 cctcatttaa tattattaca tgctccagta aatcatctat ttcatcaatc aaatgagttg
     1201 atattattat tgtccttgga ttttttatat aactatctaa aattatgtta taaaactctt
     1261 gtctatttac tgcatccaga ccaattgttg gctcatcaaa tatggtgata gctgaattag
     1321 aacaaatacc tattatattt gaaagtatag ttttcatacc tcttgacagt tgtttatact
     1381 tttttttagt atttaaatca aaatatttgc ataattcttc ttctaagcct ttatcataac
     1441 ttttataaaa actagaagaa aattcaaata tctgacctac tttaaaatct ggactataaa
     1501 attctttttc cctaacgata caaacttctt ccaaaacttt tatatcttct tttgggttct
     1561 ttcctaatat ttctatctca ccttcatttt gaattaactg atttaccatt atatttaata
     1621 atgttgtttt tccagctcca ttcttaccta ataaaccata tatcttattt ttttcaaagc
     1681 ttagagacat attattaaaa atattttgct tcttaaaact gtgagataaa ttttctatct
     1741 taatagaact actcatatat taatcctcct tgaaattaat tataatatca accaattctt
     1801 ctctatttat ttctaatttg ttagcttctt gaagtaggtt ttttacaaaa ttatgtttaa
     1861 aattttcttt tctagcttct tttataatag tttttgcacc atctgataca aacatcccta
     1921 tccccctctt cttatataaa atatttttat caaccaatat atttatacct tttaatactg
     1981 tagctggatt aattttataa agctttgaaa gctctgttgt tgatggaacc tgttcttctt
     2041 cttttattcc atttgatagt atctcatctt caattgctcg tgctatctga ataaatatag
     2101 gttcttcatt atttaatact aatttcatag gttcctcctt tctccatcag ttagttactc
     2161 aactaattaa ctatataact tataatatct tgaaaaatat tttctgtcaa tactaaataa
     2221 aaaacagagg gaaaattccc tctgtttttt gttctttata tttataatta aattttgatt
     2281 tttgtaatcc tatcccataa gttaaaaact ttaataatct gttgtatctt cattttttaa
     2341 tattttacat attgagctag ttcctaacct ttctgctcct gcctctataa atttttctgc
     2401 atcagaaatt gatttaactc cccctgcagc ttttattttt acattttttc caacatgctc
     2461 tttcataagt tttatatctt ctaaagtagc tccacctgtt cccattccag tagatgtttt
     2521 tataaaatct gaacctgaca ttgttactat ttcacacatt tttatttttt catcttcatc
     2581 taaataacat gtctctataa tgacttttaa tactttatct cctatagctt tttttatttc
     2641 ttttatttct tttcctatat tatcataatc tttatttttt atatctgaaa tgtttacaac
     2701 catatcaact tcatctgctc cattttcaat agcatctttt gcttcaaata cttttgtagc
     2761 agtagtttga tatcccaaag ggaaccctat cactgtacat atctttattt taccttttag
     2821 atattctgat gctcttttca catatgatgg tggtatgcaa actgatgcta cactcatatc
     2881 tacagcttca tcacataaaa ttttaatatc ttcccatgta gttgtagctt ttaaaatagt
     2941 atggtctact gtttttaata tatgtttcat aaaatcctcc taaatttata attaatataa
     3001 ttagattact tttttaaatg caattttctc tttgcaatgt ttagtatcat atcaacctct
     3061 tcaactttaa aaataatcat tagtagtgta taaattccac ctccaactat aactgaaata
     3121 gctagtgata caaactcatt aaatgttcct actcccaaaa ttccaaatat aaatttatac
     3181 gtaaagtatg ataaaacacc cataattaaa gatgcaacta ccgatttcaa agttgctttt
     3241 attattttat cttgtccaaa atatccaact tttctcttta ggttcaagaa gagtaacaat
     3301 atacatgcaa ttgatgatga actagttgca agagcaagac caccatgagc cattggtttt
     3361 attaaaacta agtccaatac tatatttacc cctacagata taatcccatt taccataggt
     3421 gtttttgtgt cttgtaatga ataaaaaacc tttcccaaaa tatctcttaa gccaaatgca
     3481 gtcataccaa ctgcataaaa aattaaggca gttgctgtca tttgggttgc cctcgcatca
     3541 aaagcacctc tctcaaaaat gattctaaca acaggagttg caaaaaaaat agaagcaact
     3601 gaaataggaa tcatagaaat tattatcata tttatactgg atttaactga actagtaaac
     3661 ttcttttgat tattttctgc acttaactta gataacatag gatacataac agatactatt
     3721 gaagctgtaa atgtccctgt aacaaaacca tttaatctat ctgcataagt aagtgctgga
     3781 atgcttcctt taactaatgt tgaggctaaa gttgtatcta ccaatgagtt cacttgatta
     3841 acagcaacac ctataaatac tggtgaaagt aatgctagta atttaataag gctatcatct
     3901 ttaaagttta agtagtataa gtatttgtag ttcgtttttt taacaaaaaa catgtaaaag
     3961 agcagttgaa ctaccatagc aaccacagca ccaattggaa gtatgtatgg tccaaatatg
     4021 gtacttaaca ttattgaaat aattattact atattatagg gtatacttcc aaatccaact
     4081 acaataaagt tctctttaat ttgtaaaaat gctgacataa cactcgtaat acctatgaat
     4141 attattcctg ttctgaacct cccatgacta aagtcacagg gttcctgctt catagaattt
     4201 tgcatactaa aatatgtctt actaattctc cacaggctat ccccgtagtt cctacggttc
     4261 ttacatacgt tatttatttt gaattgttat tagtcttaat ccttctttta atatatttat
     4321 acttgcattt atatctctat cgtgagtttc attacaacta ggacaaatcc attctcttat
     4381 atttaagttc ttaacttcct cgtttttata cccacattta ttgcatattt gtgaacttgc
     4441 aaagaattta cctaccttta caatttgtct accataccaa ttagccttgt attctaattg
     4501 acgaataaac tctgaccatg atacatcaga aattaagcga gatagtttac gatt
//
LOCUS       DAYAOX010000257         4445 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.260, whole genome shotgun sequence.
ACCESSION   DAYAOX010000257 DAYAOX010000000
VERSION     DAYAOX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.260"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(290..670)
                     /locus_tag="ACGI57_003358"
     CDS             complement(290..670)
                     /locus_tag="ACGI57_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9718409.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(871..2064)
                     /locus_tag="ACGI57_003359"
     CDS             complement(871..2064)
                     /locus_tag="ACGI57_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFG9718410.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            complement(2127..3383)
                     /locus_tag="ACGI57_003360"
     CDS             complement(2127..3383)
                     /locus_tag="ACGI57_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFG9718411.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNACSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASSVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     regulatory      complement(3648..3724)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT         1594 a          793 c          445 g         1613 t
ORIGIN      
        1 aatattttgt tatttttaat caaatgaacg aatattatat atatcattca taaaaacttt
       61 atataaagaa tagaaacaag ttctagatta ctatagagat gacatatcaa aatattttta
      121 aaaagtaatt taagtattag aagtaattga agttagtaaa tataaatgcg tttactttta
      181 agtattgctt aagggaataa aaaataaaaa taaactctgc aaccatctaa aaatagttgc
      241 agagcttaca aaaccaaaat tatctaaatt gtaataaaaa taataacatt tatacataag
      301 cttcttccca atttactacc cagttttcta acgactttat cttttcctct gttagctcct
      361 cttcgtgaaa tgatactaat aaattcctta taaaattatt cttcaaaaga ttttgcacat
      421 ttcttgatat aaactctaaa tatgcctttt cttttattgc aactgtataa tacatacact
      481 tgcctgtttc ttgaacatat aagaatcttt tctttgatag ttttgataaa gtttttaaag
      541 ctactttttc tggtaaatta tgttcttctt tcatataatc aataatctca tatgaattgg
      601 tttttatatc taaattccaa ataaatttca taataactaa ctctaatttt gataattttt
      661 ttaaacacat agttatcact ccgcttcttt atctatataa ttaaatatta caataataat
      721 ttcacaaaat caattctaat aatcataaca taccttaaaa tctgttctta acttctacct
      781 tcttaatctc ccaaatgatt tttttatatt gtattcaata gtataatcct tttccaaaat
      841 ataacttacc agttaaaata atctttattt ttacttactc tcgcctttta cagaagttaa
      901 taatttttta taagttacat caattccaaa agctccaaga ggtgcagtta taagtatagc
      961 tagaactgct aacgtcaaaa taagttgtcc agatgccatc cccattgcaa gtggtacacc
     1021 tcctattgct gcctgtactg tagcctttgg aatatagcca atcatacaga acaacctttc
     1081 ctttattgtc aaatttgtct taataaggca gaagaatact cctaccatac ggaataaaag
     1141 aactccaaat attaaaatta cagctccaat tcctgcttta aatgcataac ttatatctac
     1201 tgttgcccct accaaaacaa acaagagaat ttcagcgcca acccatagtt ttgaatactt
     1261 tatggacaat cttcttgcaa ctacacgata ttttatctga aggaaaatcc caatactcat
     1321 aactgctaat aatccagaaa atggcacaat accctctaaa gcagtctcta aacttatcat
     1381 taaaaatgag atacttaaaa gcaacacccc tttagcagaa tctctcatat gaaccttctt
     1441 aaaaaatact gctaaacaga ttccaatcac tgctccaact attacaccta atataattga
     1501 tactggaatt tgtaaaaaac taatagctga tatactattt ccttgtgcca atcctgtgaa
     1561 tactgtaaac atcacaatta cgaaaatatc gtctatagaa gttcctgcta aaagcatctg
     1621 tggaatactt ttttctgtac cataaccttc ttccattaat ttaagcattt taggtacaat
     1681 aattgcggga gaaactgctc ctactactgc acccatgact gctgcttcca atatagaaac
     1741 gcctagtaat tttggtgcta aaactatcat tccgataatt tcaaaagtag ctggaataaa
     1801 gcacataagt acagcaggtc ttcccacttt cttcaaatcg tttatatcta aagataatcc
     1861 agcccttgtt aatataataa tcaatgcaat ctttctaagg tcagaagaaa tatctagtat
     1921 tgaattatca atcaaattta aaacatatgg acctaatata attcctgtaa gtaacattcc
     1981 caataagtta ggtaacttta tcctcttaaa aatacctccc gaggccattc ctaataaaaa
     2041 aatcaaagct aaacttgtca acatattcct accctatcct ttctctaata ttctttctct
     2101 tctaatcata cattatgttg ttttatttat tgttttgttg aaattttttg tagtgaataa
     2161 atagatttat ccatattaaa tatatatgaa atagcgcata ctacaaagaa ataaggtaaa
     2221 tagctgtatc caaaaacttc tgcccctata aatacaggtg caaaaaaagt atttgtagca
     2281 ctaccaaaga cactagcata gccaagcgct gcaactaatt caattggcaa attaaaaaat
     2341 cctgctaaca atacaccaag actagctcca atggaaaaca aaggtgtaac ctctcctcct
     2401 tgaaaacctg ctgacaaagt cagtattgta agtataaact ttaacagcca atcaaatgaa
     2461 taaatttctc caccatagaa actattttga attaaatttg tccctaaacc tgaatatctt
     2521 cctttataaa ataaaaggaa taatacacta agacatactc caataatagc aattcttatc
     2581 ataggatttt ttaatctatt acctattttt ctttttgaca atttcagaca ccatgcaaat
     2641 gctccaccaa ccattccaaa aataattcct aaaacaataa gcttccaaaa tattgacaag
     2701 tcaaatacaa ctttagaaga aagtgcaaaa gaaaatttct ctaaccctaa agcttttgat
     2761 actgaacttg ctgtaaaaga agcagtaaac gcaggaaaaa gcgattgata ctccagtgaa
     2821 ccagctacca aaacctccat agcaaatagt attgctgcta ttggtgtctc aaataatcct
     2881 gcaaaaccag ctgccatacc agttactaga aaaatactac aagcattttt tataggcaac
     2941 cttttaccta cccaatgtga aaaagttgca ccaatctgaa cagcaactcc ttctcgtcct
     3001 gcactaccac caaataaatg ggttaaccat gtacctgata ttataaatgg aaccaatctt
     3061 aaaggaataa tttcttcttc tccatgccct acctcaaata ttagattcat tcctttacta
     3121 ctttttcctc caaatttaaa atagcaataa gcaataacaa ctcctactac tgctaaaaat
     3181 ggtatcagat acattggata tgtttctcga atatctgtaa cttttagaag aaccgttcca
     3241 aatatggtgt ctataagacc tataattgca ccaactggta tacctatgag tcctaaaaaa
     3301 aatagtccac catatgtttt ataaacgctt ttaattctta ttatcatttt aatccatatt
     3361 aaagttttaa atttttctat cattttaatt cctttctatt tgcatcataa gattttttga
     3421 aagttttatt acatcaactg tagtcaatgt aatgactatc ataattgttt tttaaattga
     3481 taggatatag gtacactctt aacccattct taataaaata attaacatat aatctactat
     3541 tttttctatt aattaaatat ttcttcaaat ttaaaaaata aaaatttagt aagtacttca
     3601 cctccatttt attgatttaa acaacaaaaa aagctggtgg catctatgat atcatagaag
     3661 tcatcagctc attggcagtt taggatatta tcatccaagg gagaacctca ttcccataat
     3721 tattatataa ttttttattt ttcatatgtc tattttatgt aaattgtatt tttaatgtta
     3781 taaataatat aacctatcaa ttaaaatagt aaagcctcta atattatgag tgttcaatat
     3841 ttcatattat ttttatgata tataatttaa atgattttct ttactttgca ttatgataca
     3901 taacttaaat atttttcctc actccacatt atgatatatt atttaaatat ttttcttcac
     3961 tctgcttata ttatgataca tgatttaaat attttcttca ctctgcttat attatgatac
     4021 atgatttaaa tattttcttc actctgcatt atgatacatg atttaaatat ttttactcac
     4081 tctacattat gatatattat ttaaatattt ttcttcactc tgcttatatt atgatacata
     4141 atttaaacat ttttcttcac tttgcattat gatatatggt ttaaataatc ttctttactt
     4201 tgtattatga tacatgcttt aaatattttt acgcacttta tattataata catgctttaa
     4261 ataatcttat tcgctttgta ttataacaca ttatttaaat atttttactc actctgcatt
     4321 atagtacatt atttaaatat ttttcttcgc tttgcattat gatacattat ttaaatattt
     4381 ttactcacct tgtattatgg tacattatct aaatattttt cttcactctg tattatgata
     4441 tataa
//
LOCUS       DAYAOX010000258         4435 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.261, whole genome shotgun sequence.
ACCESSION   DAYAOX010000258 DAYAOX010000000
VERSION     DAYAOX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.261"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            233..1393
                     /locus_tag="ACGI57_003361"
     CDS             233..1393
                     /locus_tag="ACGI57_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFG9718412.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1735..1896
                     /locus_tag="ACGI57_003362"
     CDS             1735..1896
                     /locus_tag="ACGI57_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718413.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2089..2718
                     /locus_tag="ACGI57_003363"
     CDS             2089..2718
                     /locus_tag="ACGI57_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFG9718414.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2957..4216
                     /locus_tag="ACGI57_003364"
     CDS             2957..4216
                     /locus_tag="ACGI57_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFG9718415.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
BASE COUNT         1711 a          454 c          755 g         1515 t
ORIGIN      
        1 aaaattttag taataaaata attgttgaaa aagattttaa attcagatta ttcatttcta
       61 aagtaattaa tgtgcaaatc tttattaata tatgtcaata gttaggtata tatgtattta
      121 attagcatga tatagataaa agtatttatt ttaattgtct attatctgaa aaagtcttta
      181 cttagaaatc ataaaaatca aattaattag gaaaagaaag ggtgtgtata atgtgattga
      241 gactggattt gtatattgta gtttcttggt tgtattcgta ggtgtaattt tttttatggt
      301 tgataaattg aaatggaagg tatttgatgt tattaatcca atgcttttaa tatacttagg
      361 ttcagctata cttggatcat ttggtttatt tgcacagaat gaagatgttg gaacgtatca
      421 aaacataata acagtgaact tccttccatt aatgttagtg tttttgttaa ttcaatgtga
      481 tttaagaaaa atattaaaga tggggcctaa aatgttatta tcatttttct gtgctacatt
      541 tacatttatg cttggatttg ttatagcatt cataattttt aaaaattcaa tgggtgcaga
      601 ttcatggaaa attcttgggg cagcatctgg ttcatggata ggtggctcat caaatatgat
      661 tgcagcagct aatgctctag gagttgcaga agaaggactt agttatgcaa tacttatggg
      721 ttctatagga tatacagtat ggatgtctgt ttgtctaatg tctgtgaaat ttggacctaa
      781 atttaacaaa tggacaaagt gcgatacaag ttttatagat gaggttacat caaaacttga
      841 aaaagaaaat aaagataata cagcacctac gtttgtagaa cttatgattt tactttctgt
      901 tgggtttgga gttgcgtctt ttgcacaaat tgtatccaag atacttccaa cttcaggtat
      961 acttaatgaa actatgtggg tagtacttgt tgcatctaca gtatcaatat tattgggtat
     1021 gacaaaaata tcaaagctta aagggagtac actggtagga aatgtgttta tgtatttact
     1081 ccttgcagct acaggtagta aagcaaattt ctttggattg tcagaagcac ctgtttacat
     1141 aatgtttgga ttggtagtag tccttgtaca tgctatttca tttatagggc ttgctaaact
     1201 atttaaactt gatttattta cttgtgaagt aggaagtata gctaatatag gaggagtaac
     1261 tacagctcca atgattgctg gggttcacaa tcctgtatta ataccagtag gtgtacttat
     1321 ggggcttcta ggaaatatta tagggactta ttgtgctctt atggtaactc aaatattaca
     1381 tatgttagct taataaaaat atggtataaa attaataatt taaataagaa aagtcatttc
     1441 aatttaagca gaagctttta ctgaaatggc ttttttgttt gacaagataa tctatttcaa
     1501 gaagatgttt tgacatattt agtgtataat attgtaaaat ttactaaaaa cgagatatat
     1561 ttaattttta tattgaatcg aacgtctgta tgatgtataa taaatataca gaacgaatgt
     1621 ttgcagaacg ggtgtttttt gaatttattt taataattta tgaatatata tcagaatgca
     1681 taaatatatt taaatataaa gattaaaaag aatttaaagg agtgttgata acttatgata
     1741 attccatcaa gaagaataca agatataaca ttaacaaatt taaaaaatgg tgaagtaact
     1801 ttaattgaat tggatgaaat atacaagaag atgggatttt tatttgttgt tagtgaaggc
     1861 aaattgaaaa agataaaaaa ggagaatcat cattagaaat gaatgaaata ataaatatta
     1921 taatagttta taaaacctaa cttatgtaga atataaatac ttaagttagg tttttttgtg
     1981 ataaagttta taagtactca attttataag ataaataatg gtcttaagtg aaaagtaaat
     2041 aagtttaaat tttagtaagt attatattgc ataagaggtg atattattat gaaaatagta
     2101 acctacaata ttcataaagg tatggattca aataatagat taactctaac taaaatgggg
     2161 ttatatttaa aacaattgga ttgtgatgta atttgtttgc aagaagtatt atatcctcaa
     2221 tttttagctt taaaagctgt tttaaatatg gatggtgtct ttgcgacaaa tgtaaaaaaa
     2281 gtaaatatga tatatggtat ttgtacattt acaacatcta aaatgttaaa cagtaatcat
     2341 tttttcttaa ctagcaaaaa agaacaacgg ggagctcttt gtataactat agatgcatat
     2401 ggaagaatta taaatgtaat aaatactcac ttgggacttg ataggcagga gagagcaaaa
     2461 caattagacg aaataataga ctataggaac agattagtgg ggatagttat tttatgtggt
     2521 gattttaatg agaaaaatgt ctttttaagt atgtttaatg atatggctat ttcactaaac
     2581 aagtcttact taccaacatt tgaaaaatca aactctagaa tagattacat ttttgttaat
     2641 aaaaatactg aactaaaagg atacacagta gaaaaaattt acctgtcaga ccattatcct
     2701 gtaataggtt acatttaaat ttcaatataa atgaaattag ttgttataaa ataaacaatt
     2761 ttttgttaag aaatagtaaa agaaatataa taaaaaatat caaaaataaa actaattata
     2821 agacataaat ttacaataaa gttacatttg attaaattcc ttgtttaaca aaagctattc
     2881 aaatataatc taaggtagtt ttgaaatggg aactctatta tgcaaattta agctagtgtt
     2941 aaaatgaggt gaagttatgg gcagaagaac aacaagagtt tcaagattag gtaaagaaaa
     3001 aaaacgaagt gttttaattg tattggcagc tttagttcta ataatgggtg gatttactta
     3061 tttttttaac agtaaatttc tgtacaatgg gaaaatcgct aaaaatgtat atatagaagg
     3121 agtaaatgta tctgatatga ctaaagcaga ggcattaaaa gctataacag ataagtatac
     3181 tccagaagat ttaaatcttg cttatgatgg gaaaaaatat acaatatcac caaaagatat
     3241 tgatttaaaa tatgatacag aagatgttgt aaaagatgct tatgaaagta caaaaaaagg
     3301 gtcctatttt caaaacttaa aaaaatatat tgatataaga gtgaataagg caaatatgaa
     3361 gataaaagct gaatatgatg aggcaaaact tagttccaaa gtgtcttcta tagcagatag
     3421 tataaatgtt aagatgaaaa atgcttctat tagtgttgga agtggtggac taagttatac
     3481 agactctgtt gtaggtagag aatttgattt ggcagcaaat aaagagtcta tatataatat
     3541 gataaaaaat aaagagcata aaactttaga acttaaagta aatcttcaaa aaccagatat
     3601 aacaactgaa caagtaaaaa ctgtaaattc tgttataggt caatattcaa ctacttattc
     3661 acaagctgta gagggtaggt catataatgt aggattatca gcaagaaaaa ctagtgatgt
     3721 attattaatg ccaggtgaag aattttcata caataaactt acaggaccta gtaataaagc
     3781 aaatggttac aaagatgcac ctgttatagt ttatggtaag ctagaacaat cagcaggagg
     3841 aggtgtttgt caaacatctt caacagttta taatgcggct cttctttcgg gtatggagat
     3901 aactcaagtt acaaatcatt catctgcatc tacttatgtg cctaaaggga gagatgctac
     3961 tgtgagcgat ggaggattaa acttaaagtt taaaaatcca tataagcatc ctgtatatat
     4021 taaaaattat gcaggtggag gaagtgtatc atcagtaatc tatggtaatt ctggtgacaa
     4081 accaaatata tctatagagg taaaacaaac tggtcagaat aaatattcta catatcgaat
     4141 atttaaagat tcaagtggaa aggtcataaa aaaagaacat atatcaaata gttcttataa
     4201 agaattaaaa aaatagataa ttttttagag aaatatttgt aatataaaag ggagtgtctc
     4261 aaaatgaact ttttagttta tgagacactc tttttctgtg aaatcaaata tattttcatc
     4321 atttcaacaa tacaaaagaa gcgtatctct attttgagac agcctttttt gtaaaaatta
     4381 acactattag ttcttgtttt aatattacaa tcattaatat atagaaaata ttcct
//
LOCUS       DAYAOX010000259         4432 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.262, whole genome shotgun sequence.
ACCESSION   DAYAOX010000259 DAYAOX010000000
VERSION     DAYAOX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.262"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     misc_binding    56..290
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            340..1359
                     /gene="pheS"
                     /locus_tag="ACGI57_003365"
     CDS             340..1359
                     /gene="pheS"
                     /locus_tag="ACGI57_003365"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFG9718416.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1376..3769
                     /gene="pheT"
                     /locus_tag="ACGI57_003366"
     CDS             1376..3769
                     /gene="pheT"
                     /locus_tag="ACGI57_003366"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFG9718417.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3794..4384
                     /gene="zapA"
                     /locus_tag="ACGI57_003367"
     CDS             3794..4384
                     /gene="zapA"
                     /locus_tag="ACGI57_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFG9718418.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         1743 a          520 c          915 g         1254 t
ORIGIN      
        1 tagtcacata atttgaatgg aaatgatata atactaaaat aaacaatata atattgtaaa
       61 tgcaatgaaa gaggaaagta ttttgattaa acttagtaaa gagataaaca cctaggctgg
      121 gagtgttttc taagaggtca tgagaagttc cctctggagt aacagagctg aaattttaca
      181 gtaggctttg acgtcaaaaa cgcgttaagt ttgttagagg tggtttgatg atttttaaat
      241 tgttaaacta ctagggtggt accgcgaaac tatagccttt cgtccctaga cagggattga
      301 ggggcttttt ttatacaaaa aaacgaaagg gtgatatgtg tgcaagaaaa attacttgct
      361 ttacgtgaag cagctttggc tgaaataaaa gaagcacaaa gcatagaaag tgtagaaagt
      421 ttaagagtta agtacttagg aaaaaaaggt gagataactg ccatacttaa agaaatgggt
      481 aaattatctg ctgaagaaag accagtagtt ggtaaggttg ccaatgaggt aagagaaaac
      541 attgaactta gcataaattc taaaaaagaa gaaataaatg ctattgaaaa agaaagaaaa
      601 ttaaaagagg aagtgataga tgttactcaa ccaggaaaag ttttaaaggt tgggaagaag
      661 catccaataa ctcaaattat agatgaagta acagatatat ttatcggaat gggattctct
      721 atagcagaag ggccagaagt tgagactgtt gaaaacaact ttgacgcatt aaacgctcct
      781 aaagaccatc catcaagaga tatgagcgat acattctata tcaatgatgg ggtattactt
      841 agaactcaaa catctccagt tcaagtaaga actatgagaa gtcaagagtt accaataaaa
      901 gtaattgcac caggtagatg ttttaggtca gactcgccag atgctacaca ctcaccaatg
      961 ttccatcaga tagaagggct tgttgttgga aaagatgtta ctatggcaga atttaaagga
     1021 actatggata tcttcgttga aaaattgttt ggttctgata tcaaaactaa gtttagacct
     1081 cacaacttcc catttacaga accaagtgca gaggttgatg ttacttgttt caaatgtggt
     1141 ggtaaaggtt gcccaatgtg taaatatgaa ggttggatag aaatattagg ttcaggtatg
     1201 gttcatccaa atgtgcttag aaattgtgga atagacccag aagtttacag tggatttgca
     1261 tttggagttg gggttgaaag acttgcaatg cttaaatacg aaatagatga tattagatta
     1321 ttattcgaaa atgatatgag attcttaaat caattttaat taggagggta tgtagatgtt
     1381 agtatcttta aaatggctta gagactatgt tgatatagac atggatgtaa aagagttcgc
     1441 tgataaaatg acaatgacag gaactaaagt tgaaacaata gattattatg gtgaagaaat
     1501 agaaaatata ttggttggaa agattttaga aataaaacaa catccaaatg ctgataagtt
     1561 ggttgtaact aaagtagata ttggagataa agttgttcaa atagttacag gagctacaaa
     1621 tatatcagaa ggagattata ttccagtagc tgtaaatggt tctaagttac ctggaggagt
     1681 tgaaatcaaa cagactgatt tcagaggtga attatcagat ggtatgatgt gttcagcagc
     1741 tgaactaggt atagatgaac attacattga ggagtataaa agaggtggta tatatatttt
     1801 agaccacgaa gattcttatg aattaggaaa agatataaaa gatgttttag gattaaaaga
     1861 tgctttaata gattttgaat taacttcaaa cagacctgat tgtaaatgca tgatgggtat
     1921 agctagagaa gcagctgcaa ctataggaac aaaagtaaaa tatcctgaaa tcgaagtaaa
     1981 agaaagtgac gaagagatag atttcaaagt tgagatagat aatccagatt tatgtagaag
     2041 atatgttgct agaatggtta cagatgtaaa aatagaacct tctccatatt ggatgcaaag
     2101 aagacttaca gaagcaggag taagacctat aagtaacata gtcgatataa caaacttcgt
     2161 aatgttagag cttggtcaac cacttcatgc ttttgatata aatcaagtag agactggaag
     2221 aatagtagta agaaatgcta aagatggaga gaaacttgta acattagatg atgttgagag
     2281 aacattagac aaagatatgc tagttataac aaatggagaa aaatcacttg gtttagctgg
     2341 tgtaatgggt ggtgctaact cagaaataac ttctaatacg aagactgtac tttttgaaag
     2401 tgccaatttc aaaccagaaa acataagaat gacagctaaa aaagttggta ttaggtcaga
     2461 agcatcttca agaaatgaaa aagacttaga ccctaatctt gcagagatag cagcaaatag
     2521 agctgcacaa cttgttgaaa tgttaggagc aggaaaagtt ttaaaaggtg ttgtagatgt
     2581 atatccaaat aaaccagaac ctaaaaaatt ggtagtaaat cctcaaagaa ttaaccacct
     2641 attaggtgta gatgtaccaa tggagcagtt tgtaggaatt ttagaatcat tagagtttaa
     2701 atgtaatttg gtagctaatg ataaattaga aatagatgta ccaagcttta gaacagatat
     2761 ggaacaagaa gctgatgtat gggaagaaat agctagaatt tatggatttg agaatatacc
     2821 ttctgtacaa ttagaaggaa atacgacagc aggaattaaa acttcaaaac aaaaattcat
     2881 ggatgcttta aaagataatt caactgctgt aggattaaat gaaatattaa catattcatt
     2941 tgtaagccct aagggagtag ataagataag agtaccagaa ggtaatgcaa agagaaattt
     3001 tgttaaatta cttaacccat taggagaaga aacttctgta atgagaacta ctttaatacc
     3061 aaatatgtta gatgttttat caactaatgt atctcataaa atagaagaag tatctgcatt
     3121 tgagtgtgga catatattca tcccacaaga ttcagaatta cctaaggaag aaaatagaat
     3181 gtgtgtaggt atgtatggta aagatgttga cttctttgca ctaaaaggaa ctatagaaac
     3241 tatacttgta aatgtcggat ttaaaggtta tgaaattgaa cctcaagata ataatactac
     3301 cttccaccct ggtagatgtg ctaaaatagt gtataataat aaatatgtag gtacattagg
     3361 tgaattgcat ccagatgtta tagaaaacta caatctaggt caaagagttt atgtcgctga
     3421 aatagatatt gactttgtat ttgataattc tgatagaaca aagaattatg tgccattacc
     3481 aaaatatcca tctacatcaa gagatatagc tcttattgtt aaagatgatg tatttgtaaa
     3541 acaaatagaa gatataataa aggaaaatgg tcaaggatta gtagaaagct acaagttatt
     3601 tgatgtttac aaaggttctc aaatagaagc tggatacaag tcaattgcat attcaataac
     3661 ttatagaagt aaagataaaa ctctaacaga tgaagatgta gctaaggtgc atgataagat
     3721 attaagtgaa ttaagcgaaa aattaaatgc taacttaaga tcaaattaac aactaatgtc
     3781 gggggaggtt aggatgaaca aagtaatggt taaaatccat ggcgctgaat atccaatggt
     3841 tggagacaaa tcagagaagt ttatgattag tattgctgat tttgtagaca aagaaatgga
     3901 taaaataaca cgtcaaaacc cgaaattgag cttatcagtt gctgcgatat taactgcatt
     3961 aaatatatca gaccttttat ttgaatgttc agatgagaat gagaagttga ttaaagcgaa
     4021 tgaagaattg agtaagaagg ttggagcatc taatgaagag cttcaattag aaatcaaatc
     4081 tttaaagctt acgatagcag aaaaagaagc agaaaatcgt gaagctgaaa ctaagatgaa
     4141 agagttaatt gagattatag aaaataaaaa gcaagaaata ttcgaactta gtaatacgac
     4201 agaaggttct agagcagagt tagatgctta taaaaacaag attgaggagt taagtactca
     4261 acttgaagaa gcaaatgaaa gagctactat agctgaaaac ttagcttctg agtttcaaaa
     4321 caaagcttat gatttacagt taaaatgtac agggttaaat aatgatgcaa aaaatgtgga
     4381 ataaactaaa agatttgctg tctcttgttt tttgagacag cttcttttaa tg
//
LOCUS       DAYAOX010000260         4404 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.263, whole genome shotgun sequence.
ACCESSION   DAYAOX010000260 DAYAOX010000000
VERSION     DAYAOX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.263"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     repeat_region   38..2773
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=38..67
                     /rpt_unit_seq="gttaaacagtaacatgagatgtatttaaat"
     gene            2858..3352
                     /locus_tag="ACGI57_003368"
     CDS             2858..3352
                     /locus_tag="ACGI57_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HFG9718419.1"
                     /translation="MKITGTLINYYFHCKRQCWLLGNRINLEENSEDVKIGRLLHELK
                     EEKTKYKEIAIENIKIDKLTREYLVEVKKSDADIEAVKWQVLLYLKKLKEKGIVRKGK
                     IEFIEKNKTNKKIVFVDLSEVSEEQLISIEREIVNLINSDKAPCPDLKKGCKKCAYYE
                     YCYI"
     gene            3378..4385
                     /gene="cas1b"
                     /locus_tag="ACGI57_003369"
     CDS             3378..4385
                     /gene="cas1b"
                     /locus_tag="ACGI57_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HFG9718420.1"
                     /translation="MLILGKTKYITSMGELTRKDNSICFRKNGRNVYIPVENTSEIFC
                     LNEVSLNSKLLDFLSRANIVVHFFNYYGGYSGTFYPKEYLISGKVTVAQVKTFEDRRE
                     EIAKSIVNGIRINAINTLSHYYKHGNSEIQPLLSHLKKDSINKLEKSNNIKEILSVEG
                     DIWQTFYTSFKYILREEFIFNKRVKRPPDNPLNAMISFGNSILYAKTVTAIYNTHLNQ
                     SVSFLHEPSESRFSLSLDLSENFKHVITFKTIFDLVNNRKINIEKHFDKNLNYCVLND
                     EGKQIFINSLEERLESVKEHPKLKRKVSLKTCIKLDAYKMLKCIMEEKSFIPYNIKDG
                     L"
BASE COUNT         1845 a          469 c          664 g         1426 t
ORIGIN      
        1 tttacttctc ctgttttgaa atctatatat tcatcatgtt aaacagtaac atgagatgta
       61 tttaaatata atattgatac aaatattttg ggtaaatgga cttgttaaac agtaacatga
      121 gatgtattta aattcgatat ggtaacaaat ttataaaaga tatagaatgg gttaaacagt
      181 aacatgagat gtatttaaat aaagaaaaac gaaattggtg attttgaagc aatgtgttaa
      241 acagtaacat gagatgtatt taaattagta actacaatgc ttttcctttt gaagatgata
      301 tgttaaacag taacatgaga tgtatttaaa ttaataatgc acctatttta acagtacctg
      361 gtctaaagtt aaacagtaac atgagatgta tttaaattca agtctaggag acaacataaa
      421 tagtacagat gggttaaaca gtaacatgag atgtatttaa attagtaaaa ccagatggaa
      481 ctgttgtaat accaccaaag ttaaacagta acatgagatg tatttaaatt taaaaacatc
      541 ttgaggaata tctttaacag taattgttaa acagtaacat gagatgtatt taaatgcatt
      601 ttcaggtgca tgataaatag gtaaattaga tgttaaacag taacatgaga tgtatttaaa
      661 ttttttgtaa ttagccagat tatcaactcc caaaatagtt aaacagtaac atgagatgta
      721 tttaaattaa atatcgtcct tcgtagtcaa agcaacacca gaaggttaaa cagtaacatg
      781 agatgtattt aaatcgtaaa caaaatttat caagagaaga atttctaagg ttaaacagta
      841 acatgagatg tatttaaatt tatatttgat aagagtatca aatcgactta aaatagttaa
      901 acagtaacat gagatgtatt taaattcaat cttaatacac agacattttt aaaaacaaaa
      961 cgttaaacag taacatgaga tgtatttaaa taaatagaca aatattgaat agctctgtct
     1021 tcatcaagtt aaacagtaac atgagatgta tttaaatata tttacaaaat ctctaacata
     1081 ttttttaact tctgttaaac agtaacatga gatgtattta aattctttta aagagtcctc
     1141 gggagcctcg aaaacttcag ttaaacagta acatgagatg tatttaaatt actcaaataa
     1201 atttctaatt tgtttatcta ctaacgttaa acagtaacat gagatgtatt taaattagaa
     1261 tttacaacaa tataagcctc tttaaccata agttaaacag taacatgaga tgtatttaaa
     1321 tatgttttta gtagtacatt taataaaact ttttaaagtt aaacagtaac atgagatgta
     1381 tttaaatgaa ggaaccgtac cttgataaat cacgaccgta cccgttaaac agtaacatga
     1441 gatgtattta aattgtatat ttgatgttgt tatttctcct gtgtataatg ttaaacagta
     1501 acatgagatg tatttaaatg aagttccacc cccaccaccc ccactacttt gagaagttaa
     1561 acagtaacat gagatgtatt taaattaaca atagagtaaa tataataaac aagcatacaa
     1621 agttaaacag taacatgaga tgtatttaaa tccccttttg catggtacta gacacgcata
     1681 tatggtggtt aaacagtaac atgagatgta tttaaatacg cctatatgcg gagtaacaga
     1741 tttttgcctg gatggttaaa cagtaacatg agatgtattt aaatactgtt ttgggataga
     1801 ctggaattaa atggtctgtg ttaaacagta acatgagatg tatttaaatt tttggaatga
     1861 agcatcaaca gataataaag aaaaagttaa acagtaacat gagatgtatt taaatcaagg
     1921 aaacaaaaga gaagtgaaaa gaaatataat gttaaacagt aacatgagat gtatttaaat
     1981 aatcttttaa atggaaatta agtaataaaa gagagggtta aacagtaaca tgagatgtat
     2041 ttaaattcaa ttaaataagc attaatctta gtaaaatcaa acgttaaaca gtaacatgag
     2101 atgtatttaa attttcttgt gttaattgaa cccccatttt aagctccagt taaacagtaa
     2161 catgagatgt atttaaatca gaatacagct tctatatctg gatacatttg tcttggttaa
     2221 acagtaacat gagatgtatt taaatgtgaa aagtaataca ggtatagagt ctaaacgctc
     2281 ggttaaacag taacatgaga tgtatttaaa tcaatgtatc ctctttttta gttttggagc
     2341 ctcttttgtt aaacagtaac atgagatgta tttaaattag ttagcacatt ttatttcaag
     2401 ccttaaaaca ttcgttaaac agtaacatga gatgtattta aattaatttg cacacttaac
     2461 ctctaatctt aaagtattag ttaaacagta acatgagatg tatttaaata acacaatatt
     2521 atctttaata gaattaaaca aatgagttaa acagtaacat gagatgtatt taaatttttg
     2581 ttggtcttgt gcttctttta aaagctgatt agttaaacag taacatgaga tgtatttaaa
     2641 tttaaaaagt tctgttatta aaacatccaa accaatcgtt aaacagtaac atgagatgta
     2701 tttaaattct gtaccatcta gccaaaccgt ttttgaagtt aaagttaaac agtaacataa
     2761 gatgtatcta aattactcta tcataaaatt ttaatatatt aaacaaacta atatgtgata
     2821 tacttatgta taaataacaa aataaatagg tgatgttatg aaaattacag gaactttgat
     2881 aaattactat ttccattgta aaagacaatg ctggttgctt ggtaatagaa taaatttgga
     2941 agaaaatagt gaagatgtaa aaataggtag acttcttcat gaactaaaag aagaaaaaac
     3001 aaaatataaa gaaattgcaa ttgaaaatat aaaaatagac aaattaacaa gagaatattt
     3061 agtagaggtt aaaaagtctg atgctgatat agaagctgtt aagtggcaag ttcttttata
     3121 tttaaaaaag ttaaaagaaa agggaattgt aagaaaaggg aaaattgaat ttatagaaaa
     3181 aaataaaact aataaaaaaa tagtctttgt agatttgagt gaagtatctg aagaacagct
     3241 tatatctata gaaagagaaa ttgttaattt aataaatagt gataaagcac catgtcctga
     3301 tttaaaaaaa ggatgtaaaa aatgtgccta ttatgaatat tgttatatat aaagaggtat
     3361 aagaaacata taaggaggtg ttaatattgg gaaagacaaa atatattacc tcaatgggtg
     3421 aactcacaag aaaagataat tctatatgtt ttagaaaaaa tggaaggaat gtatatatac
     3481 cagtagaaaa tactagtgaa atattctgtc taaatgaggt atctttaaat tcaaaattac
     3541 tggatttttt atcaagagca aatattgttg ttcatttttt taattactat ggtggatata
     3601 gtggaacttt ttatcctaaa gaatatttaa taagtggaaa agtaacagtg gcacaggtaa
     3661 aaacctttga agatagaaga gaagaaatag caaaatccat agtcaatggt ataaggatta
     3721 atgcaataaa tacattatcg cattactata agcatggaaa ttcagaaata caaccattat
     3781 tatcacatct aaaaaaagat tctataaaca agttagaaaa atcaaataat ataaaagaaa
     3841 tattatcagt cgaaggagat atttggcaaa cattttatac ttcctttaag tatattttaa
     3901 gagaggaatt tatttttaat aaaagagtta agagaccacc agataatccc ttaaacgcaa
     3961 tgatatcatt tggtaacagt attttatatg ctaaaactgt aactgctata tataatacac
     4021 atctaaatca aagtgtaagt tttttacatg aaccaagtga aagtagattt tctttaagtc
     4081 tagatttaag tgaaaatttt aaacatgtta taacttttaa aactatattt gatttagtta
     4141 ataatagaaa aataaatatt gaaaaacatt ttgataaaaa tttgaactac tgtgttctaa
     4201 atgatgaagg aaagcaaatt tttataaatt ctttagaaga aagattagaa tcagtcaaag
     4261 aacatccaaa attaaaacga aaagtaagtt taaaaacctg tataaaatta gatgcttaca
     4321 aaatgttaaa gtgtataatg gaagaaaaaa gttttattcc atataacata aaagacggat
     4381 tataatttaa acataaagta agta
//
LOCUS       DAYAOX010000261         4340 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.264, whole genome shotgun sequence.
ACCESSION   DAYAOX010000261 DAYAOX010000000
VERSION     DAYAOX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.264"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            231..1310
                     /locus_tag="ACGI57_003370"
     CDS             231..1310
                     /locus_tag="ACGI57_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFG9718421.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIVKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="ACGI57_003371"
     CDS             1634..2002
                     /locus_tag="ACGI57_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFG9718422.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKSLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="ACGI57_003372"
     CDS             2119..2922
                     /locus_tag="ACGI57_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9718423.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDRQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYMGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3314..4204
                     /locus_tag="ACGI57_003373"
     CDS             3314..4204
                     /locus_tag="ACGI57_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9718424.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
BASE COUNT         1657 a          480 c          816 g         1387 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagccttta
      601 tcatcaagga cgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggct gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacccg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgtaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctac taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagcc atataaatta gaatttgtag
     1621 aggagaaata taaatgaatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaatca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccag atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gatagacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gttacgaaaa aaaagaactg tgtcatctca tgtatatggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat atataaattt
     3061 ttaagcgaaa tacatcaaaa gaaggattag aattagtgga gacagaacag ctattataga
     3121 ttataaataa atagttttaa aggagaggta ctacgaatat agaggaaggt aaatcatacg
     3181 ttattcaagt attataaatg attagtttaa aagtactatc ggataggagt ctccatattt
     3241 aaaccttagt aaaggtagtt aaagtagtag tttaaaaact aaaaaattat ataaaggaat
     3301 taggtataag aaaatgattg agagtatacc agcaaaacaa attttacaaa aagtgaaatt
     3361 tgacaataca agatggtttg gaattgacta taatatgaat ctctatcgtg gttgttctca
     3421 tggatgtata tattgtgata gccgtagcac aatatacaac atagagaatt ttgacaaagt
     3481 tagatataaa gaaaatgtga tagaaatttt aagtaaagaa ttgcgttcaa aaagaaaaaa
     3541 aggtgtagta ggcattggag ctatgtctga cacatataat ccttttgaaa agcaactttg
     3601 tattacaaag caagcactag atttgatttc ggaaaatcat tttggagtgt ctattgatac
     3661 taaaagtagt ttggtggtta gagatatacc gattttgcag aaaattaaaa aaaataattc
     3721 tgctattgta aagttgacta taaccactgc aaatgatgaa ttatcaaaga aaatagaacc
     3781 ttatgtaaat ccaagttctg tacgatttga cgcagtgaaa gaacttaatg attctggaat
     3841 tttttgtggc atattattga ctcctatgct tccatttttg actgataata aagatgaaat
     3901 acgagcaatt gtagaaaaag cacacaaggc taatgcaaaa tttatttatt gcatgtatgg
     3961 tgtaacaatg cgtagtggtc agagagagtt tttttatgag catttaagag atatatcacc
     4021 aaaattggta cttaaatatc agaaaacata tggtttaaac tatgtatgta ctgtacaaaa
     4081 taaagattct tgtgagaagt tgttaagaga agaatgtgca cgttatggta ttttgacgga
     4141 tatgaaagat attataaaag cgtacaagag gtcggagtca tatactcaaa taaaattttt
     4201 ttaagatagg ctttggatga gatataatag tgttgattga tgtgttgata ttatagttga
     4261 ttgttagaga ggatattata gatgaatatt agtattgata ttttaggtaa ttgttagagc
     4321 tgatattata gatgaatatt
//
LOCUS       DAYAOX010000262         4327 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.265, whole genome shotgun sequence.
ACCESSION   DAYAOX010000262 DAYAOX010000000
VERSION     DAYAOX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.265"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            632..1309
                     /locus_tag="ACGI57_003374"
     CDS             632..1309
                     /locus_tag="ACGI57_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9718425.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            1381..3396
                     /locus_tag="ACGI57_003375"
     CDS             1381..3396
                     /locus_tag="ACGI57_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9718426.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLYFL"
     gene            3446..4126
                     /locus_tag="ACGI57_003376"
     CDS             3446..4126
                     /locus_tag="ACGI57_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9718427.1"
                     /translation="MQTKILIIDGDKDNCKKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
BASE COUNT         1722 a          421 c          715 g         1469 t
ORIGIN      
        1 attgggcatc tagagatata tggagttagt ggtgcaaccg gctatgaata taatttttat
       61 attgcatagt ctttttttgt tttaaaatta acaaatatgt tgcaaaattt gaaaataaga
      121 gtattagtcg ttaagatttt tattgatagg taaaattttg gcttttaaag tagccatttg
      181 ttggaaaagg gaaatttttt taaaagaata atagtattat aataagaaaa taaagttttt
      241 ataaacattg agatgcagaa tataaacctt ttgtaacaat atatttacca aaataataca
      301 atttatttta tattataacc atataaatca tataaatcaa tactcaaact agccaataga
      361 caagtttcta gaaaaataat aaatttatac gtgaaaatga ctttaagctc cattctccaa
      421 caatcggaga ctttaattag tatttaataa tgaatcttaa tgtttaatac aatattcaag
      481 aattattcaa aaatacttaa gtattatagt ctagtttgta aagatattat aaaataataa
      541 taccagttat atatgaaaat aaaacattat aatttgtaat aactataaaa cttggaatta
      601 taattaaaat acctttgaat ggagtggtgt aatgtataat ttactggttg tagatgatga
      661 tgttgatatt ttaaacataa atcaaattta tttttctaat ataggtttta atgtatatac
      721 atcagagact gctaaagatg ctatggatat agtagagagt atagttttag actgtataat
      781 tttggatatt tcattgccag atatagatgg atataaaatg tgcaatgata taaaagaaaa
      841 ggttaacata cctataatat ttttatctaa ttatgagcat gaagaagaaa aagttcgggg
      901 atttttagct gggggggatg attatataac aaaaccttat agtttaaaag aacttgaact
      961 tagaatatat gctcgtatga ggcagtacaa aaatattaca tcagttccta atgtgttaaa
     1021 gttttcagcg ttagtaataa atgtaaattc aagaaaagta acttttgatg gagaaagtat
     1081 agatttaact actatggaat ttgaaatatt attgtttcta gctgaaaaca aagaacaagt
     1141 attttcgaag atagagatat ataaccaagt ttggaaaatg cctgatattg gagatcaaca
     1201 tacagtgcag gttcatattg cacagatgag aaaaaaaatt aattctttaa gcagggagca
     1261 tcaatacatt caaaccgtat ggggaaaagg atataagttt gtaccataag tgagatactg
     1321 taattaataa atagttcttg ttttatttaa tactactatg aaagagatgt ggttgataca
     1381 atgaaaaagt ataatatact aaaaggaact ctcattgtat tgatagtctc tctgttggca
     1441 ataacaggat taaatcttct gtataaatat gataataaat atacatataa gtctattaga
     1501 gcaagtaatg gaattttaac tatagataat gatactttca ctaaaggtaa acttgtcttt
     1561 ttgatagatg actgggagtt ttataatcat aaaatattta aaccgaatga ttttacagag
     1621 caagatataa aaccagaata tgtttttata ggtcaatatc cagatttttc tatgagaaag
     1681 gggactcagt caccatatgg gaatgctact tatcgtatga gaataaaaaa tgaaggcaag
     1741 gataagctac taagtttaga gcttcctgag attttttccg caagtgaagt atggataaat
     1801 ggagaaatgg tttctaaatt aggagatgta ggaacagaaa aatatagacc caaaattaaa
     1861 aattcagtag taagttttat tgcaaagaaa aatacagaaa ttataatcaa tgtatcgaat
     1921 ttttcacatt attatagtgg tttgtattat ccgcctgcat taggagaaac aaaggacata
     1981 agtaatatga tgttttatcg attacttttt tatagtataa tttgcttcac aacttttgca
     2041 atagctatat tttcattgtc tgtatggttt ctttcaaaca aaagtaaaat ttacttatat
     2101 tttggatgta tgtgtgtttt ctttgccatc catgtatcct atccctttgt acatttgtta
     2161 ggactgcctt tggtaagctt tacatatgcg gttgaagatt tgtcatattt tatggtagtt
     2221 ttatgtatta tttgtataaa tggaagtata tccaaaatag aagaaaataa agtgtacaga
     2281 tttttaattt taccattagg aattacaatg agtatcatat ctatagtgat accaattatt
     2341 cttcttccat atgatatgta ctttgtcaac atatatggga agttaataga catatataaa
     2401 tatattgtat ttatatatat attaatgtct tcgctggttt caatatataa aaagacatgc
     2461 tctgagcata taagttctta tattttagtt tcagtaaaca ctgtttttgg aattagcata
     2521 ttatttgatg cagttacgag taatagattt gagcctatat ttacagggtg gcaaacagaa
     2581 tattgtgggt ttgtaattgt tattttattt tctgttatga tgataaacag gaatagaata
     2641 ataataaaag agaatgaaaa acttacaagg aatcttgaac atgaagttga agtgagaact
     2701 aatgaactta caacattgtt aaatgaacgt aaaagattct tggcagatgt agcacatgat
     2761 ttaaaggcac ctgtttcggc tatacaagca tttatagatt tgataaaggt tggtaatatt
     2821 catgtagatg aagagacaag aacctatctt tatgctataa atcaaaagtc aaatgaagta
     2881 caaaatagag tacgtagttt gcaagaattt acatcacaag ataaaagcat tggtattagt
     2941 gaaaaaatat gtctgaataa ttttattaaa gagatatatg aaaataattt gccagataca
     3001 caagcatgtg gtgtaaactt tgaaacttac gtattggaga aaaaaatata tgcaaagata
     3061 aataaagaga tgcttacaag agcatttgag aatttaattt ataatgcttt gacctttact
     3121 ccatttgatg gaaagataat tttatcaatg gataaagaag atggatttgc aattattaaa
     3181 atttctgata atggaaaagg tatacctcca gaaaatatac caaaggtatt tgacagattc
     3241 tttacagaaa gagacaataa tgagccaaaa gggcaagggt tagggctata tattgtcaaa
     3301 tccattataa gagagcatgg tggagaaatc tttgtagatt cagaactagg aaaaggaact
     3361 gtttttacaa taaaattaag tctatatttt ttataataat agcttattta atataaaaga
     3421 ataatgatat ttgggagtga ttataatgca aactaagata ttgataatag atggagacaa
     3481 ggataattgc aagaagctaa aaggatttct ggaggaaaaa ggaataagta tagatttagc
     3541 atataattgt gaagaagcta ttggaaaaat atttagcaat aaatatgatt taatattttt
     3601 agaaataatt ctaagtgatg gtgatggatg gactctttgt aaaaaaatta gaaatgtaac
     3661 tacttgtcca attgtttata tgacttatat aaatgaagac caaagcattt taaatgcttt
     3721 aaattctggt ggagacgatt acttaataaa gcctctaaat ttagaaatat tatacgcaaa
     3781 agtaaaagct attttaagaa gaatgaactc atatgtaaat aataatgaaa ataatacaaa
     3841 attagaggaa tataatagaa ctagtggagt aataaagtta gaagataaaa taataaaatt
     3901 gactccaaca gaaaataagt tgttaaactt ttttatagag aatcctgaaa agactttgac
     3961 tactaaagag atatatgaac atgtatggat gaatgaatac ttagaggata actatagtgt
     4021 ggtagttgca gtgaatggat taagaaagaa aatagagaaa gattataaaa atcctcagaa
     4081 aattataaca ataagaggag caggatatta ttttaataaa gaataataaa tatattaata
     4141 tcattaataa ttgttataaa tgtgaaatac taacaatatc taaactaaat tattataaaa
     4201 tagctgactg taatttaatt gataaagcct attcttctag actttgccag ttaaattaca
     4261 gtcagtttta ttatataaaa aaatgcgtaa tggaactatt attttataga ttatatttga
     4321 aaagata
//
LOCUS       DAYAOX010000263         4250 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.266, whole genome shotgun sequence.
ACCESSION   DAYAOX010000263 DAYAOX010000000
VERSION     DAYAOX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.266"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            491..1765
                     /locus_tag="ACGI57_003377"
     CDS             491..1765
                     /locus_tag="ACGI57_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFG9718428.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1972..2298)
                     /gene="trxA"
                     /locus_tag="ACGI57_003378"
     CDS             complement(1972..2298)
                     /gene="trxA"
                     /locus_tag="ACGI57_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFG9718429.1"
                     /translation="MYIMVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYE
                     ALGNEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKN
                     KIESYM"
     gene            2556..3308
                     /locus_tag="ACGI57_003379"
     CDS             2556..3308
                     /locus_tag="ACGI57_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFG9718430.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3295..4176
                     /locus_tag="ACGI57_003380"
     CDS             3295..4176
                     /locus_tag="ACGI57_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFG9718431.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNNITS
                     VKIGGSAYTVLSGEIMY"
BASE COUNT         1661 a          545 c          570 g         1474 t
ORIGIN      
        1 atttttttat aatttttttt ataaaattat ttgtattata ttttgtattt tttataaaat
       61 tattatactt gcacatcttt tataacatat aaaatctttt tttctaatta tatacaatat
      121 attttccatt atgttctaat caatttcttt actattttta caactttatt agtcatattc
      181 ataattattt ctaattagtg tttaacattt tatgtaaaat ttataaaaaa atgtatataa
      241 attaaaataa gtgactatac taaaaatatg atacggataa acaataagtt caacctatct
      301 cccccaaaat caagtgagct acagtttccc ccaactgtag ctcattttcg tgtaatttaa
      361 taaacatctc aacaagaatt aattaacaaa accccttata aaaacataac atatattaaa
      421 caagctcttg attaaaactt gagctttttt aataaaaaac agaatagttt attagataag
      481 gggttttttt atgcaattca tggatgtatc taaatacatt gaaggtgcaa accttaaagt
      541 taaaactaaa agtggtgaaa ctgttaaaag aatatatttt aacaactctg ccactccatt
      601 agttttaaaa aacgtagtgg ataacttaaa ctgtgagatt cctttgctta cttatattaa
      661 tgctccagga ataatctcag aaaaaaatac tttaaaatat gaaaatgtac gctctactat
      721 cctcaaactt attgatggag acgaagaaaa ggattctgtt atttatgtca aaaatgctac
      781 agaaggaata aacttcttag caaaattttt taaagatgaa aatccagata aatttgttat
      841 taccacagca atggagcata tggctaatta cctgcctttt aaagttaatt ttcctacaaa
      901 agttgttgga attacagaaa aaggagaact tgatttatgt caattagaga atatacttaa
      961 aaattatgct ggtaatgtat ctctcgttac agttacagga gctagtaatg tcacaggtat
     1021 aaccactcct atatatgaaa tagcaagaat agctcataag tatggtgcaa agatattggt
     1081 ggatattgtg caagtaattc agcataaacc tttttcaatg atgccacatg aatgtgatga
     1141 gcatatagat tttgttgttt ttagtgctca taaatgttat tctccactaa atggaggagc
     1201 tctggtagga cctaaaaaat tctttgaaaa atttaatcct gtactatatg gctcaggttc
     1261 taccaaattt gtaaatgata acaaaatact atatgcaaac gtaccccaac gttttgaagc
     1321 aggataccca gagtattttg gaactcttgc aatgggaaaa gcactgaaca ctttaaataa
     1381 aattggtcta aacagaattt caaaatacga aagagaatta tttttatatg ctaaagaaga
     1441 aatgagtaaa atacctaata taataatata tggagataaa tcaaataatg taattatccc
     1501 aaatatatct tttaatataa gtaatatgta ttacaaagat gtagcaaaat atttagttga
     1561 taactttgga atagaaactg gagctggagt agtaggtgca gatatttatg tacaaaaatt
     1621 attaggtata agtccaaaag aagcatatgt cagatttatg agggaaaatc ctgtaggtct
     1681 tgttaggata agcttaggaa tgtacaatac ttttgatgag gtggatagct ttatacatgc
     1741 tattaaaact ttggctttta aataagtttt ttctattctt ttttatcaat aaatagagag
     1801 tagttttgtt ttcacaaaaa agcaaattat atcaacagtc aatttcatta gcaattagac
     1861 actcatttgt aactatacaa actattttac actatacaaa caaaaaactc attacaatga
     1921 gtttttttgt ttatatatga gatttatttt aattacttca atttacttta attacatata
     1981 tgattcaatt ttgtttttta aattctcctt aggcataaaa cctattaatc tatctacagg
     2041 ttttccatcc ttaaatatta gcatagttgg tactgtagat acttcaaatt tctgagctaa
     2101 ttctatactt tggtctatgt ccaccttcaa gaagtttgct ttttcaacca tctcatttcc
     2161 taaagcttca tatattggtg atagcatctt acaaggtcca caccaagtcg caaagaaatc
     2221 tacaactact aaaccatctt tattttctac ttcatttata aattcattat tatttactat
     2281 ttttaccata atatacatct cctttattat cttcttgata ttaatatact acgacctata
     2341 taacagttca attaggcact tttttgtaac cgcctatttt taatagattt aatattatga
     2401 taaatagatt tataagatta aatatttata agactaaata tttctaatat attttatttt
     2461 tttcttaata tagttgacat tttttccctt aaattataaa atcaaattaa taactacata
     2521 ggtagttact aatcttttat caaaggagtt tctatatgga taatattatt aatgaattac
     2581 aaaatatagg atttactaaa tacgaatctc aaatatacat atcattatta aaggaaagcc
     2641 ctttaacagg atatgaaata agtaaattat caggtgtacc acaatcaaaa gtctatgaaa
     2701 atataacaaa gctattaaac aataatataa ttatcaatgt tggaactgac cctattaagt
     2761 acataccaat aaaccctaat gaattattaa aaaataagaa aaaagaattt gacaattcta
     2821 tggataatct aaagaatagc ttacagatat taaataaaag taaaagtgta gaatacgtat
     2881 taaacataaa aggagataaa aatatattaa aaaaagcaat agaaatgata aaaaatgcaa
     2941 aacaagaaat tataatttct tgcccttatg aagaaattct tgaattaaaa aaagaactcc
     3001 taattgctta caaaaaaaat attaatattg aaatactact tttagaaaac aaaagtctga
     3061 aaggtcttga taatattcat attcatggag gtaataaaac tacacttgaa aataaaatgc
     3121 tttacaaagg aattattttg attgtagata atgatgaaat attgacagga aacttatcag
     3181 aaggcagtga ggccatttca atttggacaa aaaactctaa cattttatat attggagttc
     3241 aatacataaa acatgaaatt tatatttcag aacaaataaa aggaggttat aaaaatgaac
     3301 aaaattaaga ttaaacaagt tgatgctttt acaactatac cttttggtgg aaatcctgct
     3361 ggtgtcgtta ctgatacctc taacatttct gatgaaatta agcagaatgt ggctaaagaa
     3421 atgaatttat cagaaactgc atttgtttca gagtcaaata ttgcagattt taaagtacaa
     3481 tttttcacac caggtttaga agttgattta tgtggtcatg caacaatagg tactttccat
     3541 gcacttttgg aagaagggaa gcttaatact aataaaagta tattttatca agaaacaaaa
     3601 gctggtgtat tatctgtaga gcttaagaaa ataaataatg aaaatatatt tatgatggaa
     3661 cagaatacac ctgaatttga aaatttagat aattgcagaa aagaacttgc acaaatgata
     3721 ggtgtgcatg aagatagact tcttgaatat cctattataa aggtaaatac tggactttgg
     3781 tggcttgtat ttggtctaaa atctttagat gatttaaaaa atatctctcc agatttaaat
     3841 aaaatcaaaa aatttagtgg tgataataat ataattggta taactccatt ttgtatagaa
     3901 actatagata aaaagtgtga ctaccattta agatctatag caccatatgt tggagttaca
     3961 gaagaccctg tatgtggaac tgggaacgct tgcgtatcat cttatatagt acatcacaat
     4021 attttagata aaactagttt tattggtgag caaggtaact ttataaacag acctggaaag
     4081 gtatatgtaa atataactag agaaaacaat aacataactt ctgtaaaaat tggaggaagt
     4141 gcttatactg tcttaagtgg tgaaattatg tattaatccc atacctaata aaaacaaata
     4201 aaaatactag ctaactaaag ctagtatttt tatttgttat ttttatttaa
//
LOCUS       DAYAOX010000264         4246 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.267, whole genome shotgun sequence.
ACCESSION   DAYAOX010000264 DAYAOX010000000
VERSION     DAYAOX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.267"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(311..1423)
                     /locus_tag="ACGI57_003381"
     CDS             complement(311..1423)
                     /locus_tag="ACGI57_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9718432.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLLTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDFIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            complement(1549..2553)
                     /locus_tag="ACGI57_003382"
     CDS             complement(1549..2553)
                     /locus_tag="ACGI57_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9718433.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            complement(2841..3689)
                     /locus_tag="ACGI57_003383"
     CDS             complement(2841..3689)
                     /locus_tag="ACGI57_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFG9718434.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            complement(3725..4195)
                     /gene="trmL"
                     /locus_tag="ACGI57_003384"
     CDS             complement(3725..4195)
                     /gene="trmL"
                     /locus_tag="ACGI57_003384"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFG9718435.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
BASE COUNT         1320 a          806 c          423 g         1697 t
ORIGIN      
        1 taaacttaag cagtatattc cgtacagtta gcacaaattt aataaatata tctttttatt
       61 gctaatctaa actcaatcaa tatattttgt acagttagac taaatttaat aaatatatcc
      121 ttttatcacc aatctaaact taagcagtat attccatact gttaacacaa atttaataaa
      181 tatatctttt tatcactaat ctaaacttaa tcaatatatt ttgtacagtt agcacaaatt
      241 taataaatat atctttttat tgctaatcta aacttaagca atatattttt ataatcttct
      301 acatcaataa ttaatatgta tctttcactt tgttatactc tttttttatg tattccaaaa
      361 catctggtgg tatatttcta ctgctctttg aagtcaagcc aagttcccca tcttttattt
      421 taaattccat tactttactt cccttaaaac catcttttgc aacgttcttt atcagttcat
      481 atacaggttg ttctacatct tttataattg aacttattac attatctgga gccatatcgt
      541 attggtcttt attaatagct ataactttac caccattttc tcttgctacc tgaatagctc
      601 ttttactatc atcttctgta gctgaaaata caacatctct atcagaatct ctcatttcct
      661 ttgcaatagt tctagctgat tcagggtttt caaatatatt tgaatactta actagtaact
      721 cagcatcttt atttgaagtt ttaagcccct ttctaaatcc agattcatac ttatctctac
      781 tagaaccctc aactcctcct ataaaaccga ctttcccagt atcagtcatt ttacctgcaa
      841 taagtcctgc taaatatcca gtaacattat cttgaaatga tacagaaatt acattagctg
      901 gctgaacttt ataagtatag tcaactattg caaattgctg tttgggatat ttagtagcag
      961 cttctttaat tggctctgca aatctggctc caactgctat tataaaatct ggttcatact
     1021 tatgcagttt ttctatactc tctttaaaac ttttagaagt ctttggtttt tcaagtttta
     1081 tcctaacatc aaaatcttcg tcagcccttt ttaaggctga tagtacattg ttattatatg
     1141 attcgttatt taagttttcg gtatctaaca atactccaat agtttttctt acatatgaat
     1201 catcttctgt gtcatcttct ttattcttac ttttactatt tgatttatcc ttttctgctg
     1261 aagttagcaa acttttagac tcactctgtt tcgatttagc actatctata ttagaattat
     1321 tcttatttac tttatcaaac ccaaaatcaa aattacatat agctaatata aaaattgtaa
     1381 aaactactac aaagcaagcc atcaatatcc tttttatttt cacaaaattc actccctgat
     1441 ttagataggt ctgacctacc tctgcatatg tctttctata tatgtctatt ataacatcca
     1501 attcacctca taactaaact cataagtatt cttggatatt atagtagatt atttattgtt
     1561 cttattaaca tattctaaca catctggagg aatattttga gaactatttt gggcaagtcc
     1621 agtacttcca gacttaactg tgttttctat aacctttccg cctttataat ttccttttac
     1681 aaaactttgt atcagattat aagtaggatt ctcaaaatct tttacaatag aacttataac
     1741 attttctgga gctaactcat gttggtcttt attcgttgct ataacttttt tattttttgc
     1801 ccttactgca tctattacag cttttgaatc atcttcagta gtagaaaaaa taatatccac
     1861 tccattatcc atcatttttt tagctataga ttctactgat ttgctatctt taaaaacatc
     1921 tgcatactca actgaaatat cctttatact actattagaa aatttaacac cctctctaaa
     1981 acctgattca aatttatctc tactagaact ctttgttcct cctataaaac caaccttatc
     2041 gctttcagtc atttttccag caattaatcc agctaggtat ccagatttat tatcttcata
     2101 agaaattgaa gttatattag aaggttgctt gtcatactca tagtctataa ttgcaaattg
     2161 ttgctttgga tatttcttgg ctaccatctc caatggtttt gcaaatctag ctcctacagc
     2221 tataattaaa tcgtaattgt cattacataa aatatctata ctattagaaa aagttgagga
     2281 atcatctgat tccactttgt ttgtatctat attaagtttc ttttgagcat tgtttaatgc
     2341 taataaagct gaattattca tagcttcatt atttgttcct tcaatatcta gtatcattga
     2401 tacagatttt tttgaattac ttttatcgct tatatcactt ttattatctt tgctagagct
     2461 aggattatta cttgggttat tagcacaccc aaccaacatt ccagctatca ttgctacagt
     2521 taacatcaat atcaatattc ttttgaaacg cattaaattt cactcctcat acatttacga
     2581 tatttttata tcctatttac ttttacatcc tatttataat ttttttatat acaatttaca
     2641 agtacatttt gaaatttttt taataaatct tactataaaa aagtgtgcaa tagattctta
     2701 tctatttaca cacttcccta caaactaaat ctaatatatc aacacttaat ttcatccaaa
     2761 ataaatccaa tttaacatct caacaattaa tattctatcc taatttaata aaaaaataaa
     2821 taatgtcatc caaaaatata ttatttattt tttaagcata tgataccagt aacaccagga
     2881 ccagcatgtg cacctataca cacaccaatt tggaaaaatc taatttcttt aggtcttaat
     2941 tcatcttgca taaccttaga taattgttct ttttccttaa aatcatcact atatcctata
     3001 tacacaattt ggtctgataa atcatcacca caatttgatt ttatcaattc catcatctta
     3061 gaaattacat tcttctttcc tcttacttga gatatttgcg aaactaatcc atctcttatt
     3121 tcaagaatcg gttttatatt taatatactt cctattgctg ccttagtaga tgatattcta
     3181 cctccctttt gtaaatattc caatgtatct actgaaaata taacaaaagc cttctctttt
     3241 aattcatcca aaacaggcaa tatttcagca acagactttc cttcacttgc aagttctcct
     3301 gcttttatta caagtaaacc agttcccata caaagcaagt tagagtcata tgtatatatt
     3361 ttaccatcta catcattctt tgccattaca gcactttgat acgtacctgt agctgtggca
     3421 gaccctgcta tataaagtat ctctctatcc tcatttgtat atttctcaaa aatttcctta
     3481 aattgggcat aagtagcttg agaagttttt ggatatacat tttcttctct aagcctttta
     3541 taaaattcat ctccagatat atcaattcta tccttgtatt ctctttcctc cagtataact
     3601 gttaatgaaa ctatatctat atcatattta tctaacaaat cttttggcac gtccgataaa
     3661 ctgtcacaga ttatctttag atttttcatt aagtatgctc ctttctcatt taggcttgtt
     3721 gaaactacct catatttggg tatcctattt gtcttaaagc ctcgtacact acaatagcaa
     3781 ctgaatttga taaatttaat gacctagctt tttcaacatc caacataggc actctaatac
     3841 aagtatccat atttgctttc aacaaaggtt ctggtaatcc ctttgtttct ttcccaaata
     3901 ctataaaaca attttcttca tatttcacat ctgaatgtga ctgtttagcc tttgttgtag
     3961 aaaaaaagta tttactattt ggatactttt cttgtaattc ttcaaagcta tcgtaatatt
     4021 gtatatcagc tatatcccaa tagtcaagcc cacatctttt taggtgttta tcatccaatg
     4081 aaaaacccag tggttttact aaatgtaaag ttgaacctgt tgctgcacag gttcttatta
     4141 tatttcctgt attttgaggt atttctggtt ctactaatac tatatttaat gacattttaa
     4201 tcctccctaa gtatcgcttt tatttatcaa attttatttt ataaac
//
LOCUS       DAYAOX010000265         4234 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.268, whole genome shotgun sequence.
ACCESSION   DAYAOX010000265 DAYAOX010000000
VERSION     DAYAOX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.268"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            477..1385
                     /gene="trxB"
                     /locus_tag="ACGI57_003385"
     CDS             477..1385
                     /gene="trxB"
                     /locus_tag="ACGI57_003385"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFG9718436.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCITSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1475..3298)
                     /gene="typA"
                     /locus_tag="ACGI57_003386"
     CDS             complement(1475..3298)
                     /gene="typA"
                     /locus_tag="ACGI57_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFG9718437.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3510..3752
                     /locus_tag="ACGI57_003387"
     CDS             3510..3752
                     /locus_tag="ACGI57_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718438.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3900..4091
                     /locus_tag="ACGI57_003388"
     CDS             3900..4091
                     /locus_tag="ACGI57_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HFG9718439.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1549 a          549 c          673 g         1463 t
ORIGIN      
        1 gtgctaatat ttaaactatt aatcataatt ggttcaacaa aagtatatag ttaataatgt
       61 ttaatttgaa gaaattgtta gtgtgaaatt acatatatat tcaaattaaa ttcataaata
      121 tttcacataa taatagtata ctgtaaacaa aaggaggtat taaatatggg aatagtactt
      181 ataacagcag cattagtttt ggttatgtat ggactttcaa ttctatcata tatatcagaa
      241 agcaatatat tagaagacac tgtaaatgat aaccaataag gttttgaatt atataaataa
      301 gtgagaactg taaataaaag ttaattttta tttgcagttt ttttatatat atgtaaaata
      361 aagttaatta ataaaaaata atgttttgtt tatagtatat atggataata atagtaaagt
      421 ataagaatga tgagaataat atataagaaa taataatata aagagaggta aacgttatgg
      481 tagatatcat tgtaattggt gctggaccag caggtttaac ttcagcaatt tatgctatga
      541 gagcagggtt aagcgttaca gtatttgaaa aaaatattta tggaggtcaa gttgcaagta
      601 cctctgaggt agaaaattat ccagcagttc aaaagatttc tggggtagaa ttttctaata
      661 atatatacaa tcaagcagtt gctcaaggtg ttgatattca gtttgatgag gttgaagaga
      721 taaatttaga gggaaaagta aaggttgtaa aaacttcttc aggtgaacat aaagcaaagg
      781 ctgttattct tgcaaatgga gttgaaagac gtaaattagg ttgtgcaggg gaacaagaat
      841 ttacaggtag aggagtatct tactgtgcaa catgtgatgg agcctttttt aaagataaag
      901 aagttgctat agtaggtgga ggaaatactg cactagaaga tgctttattt ttagctaata
      961 attgtacaaa agtgtattta attcacagaa gagatagttt tagaggtgaa gaagtgttgg
     1021 agaaatctgt gaaagcaaga gagaatattg agatacttta cagtcatggt gtagaaaaaa
     1081 tagaaggaga aaagactgta tcgaaaatag aagtaaaaaa cttaaagact gaagaaaaaa
     1141 gaactataga tgttagtggt atttttattg ctataggtct taaacctaat aataaaatgt
     1201 ttgaaaatgt tcttgatttg gatgaaggtg gatatataat ttcagatgag tcttgtataa
     1261 catcagtaga aggtgtatat gtagcaggag atagtagaac taagttttta cgtcaaataa
     1321 taacagcagc atcagatgga gctattgcag ccgttcaagc agctaattat ataaatgttg
     1381 aataatataa atttcaaata aaaatagcca tataaactga aataattttt cagtactata
     1441 tggctatttt gttaattaga ggtaaatgta cgttttattt accttgtcta gatttgttat
     1501 atcttaatct atcatgttca tttaagaatc tttttctaag tcttatagaa tcaggtgtaa
     1561 tttcaactaa ttcatcatct tctataaact ctagtgcttc ttcaagagta aatattctag
     1621 gaggagataa ttttatagca tcatctgaac cagaagcacg tacgttactc attttcttgt
     1681 ttttacaagg gttgactacc atatcatctt ttctggaatt cataccaatt atcatacctt
     1741 cgtaaacttc tacaccaggg tctacaaaca ttacagctct atcacttaag gcattaagag
     1801 aataccccat agtaacacca ggtccttgtg ctattaaaac accatttcct cttgaaggta
     1861 tttctccttt aaattcttca aatttttcaa atgaacgaac taaagtacct tctccacgag
     1921 tagcgtttat aaattcactt cgatatccta aaagaccacg agtaggtgct aagaactcta
     1981 tttttacata gtctccttct attgacatag actccatcat accttttctc atgtttaatt
     2041 catttataat agtaccagag taaacttcag gacagtttac aacaactctt tctattggtt
     2101 ccattaattt tccatcttct ttatgcatta aaacttcagg tttagaaaca ccaacttcat
     2161 aaccttcacg tctcatattt tcaagtagta tagataagtg aagctctcca cgtccagaaa
     2221 ctttgtatcc atcagtagta tcaagaggtt caactttaag accaacattt acttcaagtt
     2281 ccttttcaag tctatctttc aaatgtctag ttgtaacgaa ttttccactt tttccaacga
     2341 atggagagtc gtttactaag aagttcatag aaagagtagg ctcttctatt tttatcattt
     2401 ccataggtag tggactatct aaatcgcata tagtttcacc tatagatata tcaggaatac
     2461 cagctataac aactatttct ccactagtag cttcatcaac ttcaacttgt tttaaacctt
     2521 catacacaga aagttttgat acttttcctt tagatacaac agaatcttct ctacatatag
     2581 caacttgtgt attgttttta agagtacctt tgtaaattct acctatacca agtctaccta
     2641 cataatcatc atatgcaaga gctgatattt ggaactgtag aggttcatta tcgtaattag
     2701 gataagcttc aacgtgattt acaatagttt taaatagagg agaaaggtct tcactatcat
     2761 catccatttc aagtttagca ataccttgtt tagcaatacc atatattata ggaaattcgc
     2821 attgctcatc tgttgcattt aagtctacaa ataaatcgaa aacttcatta actacttctt
     2881 ccgctctctg gtctttttta tcaatcttgt ttataaataa tattggtttt aaaccgaact
     2941 ctaaagattt ttgtaaaacg aatctagttt gaggcattgg gccttcacta gcatcaacta
     3001 atagaataac tgtgtcaaca gttttcataa cacgttcaac ttcagaagaa aagtcactgt
     3061 gtcctggagt atcaactatg tttattttgt aatcttcata gtttattgca cagttttttg
     3121 aatatatagt tatgcctctt tctttttcaa ggtcgttact gtccataacg cagtctttaa
     3181 ctacttcgtt ctttctaaaa acaccgcttt gagaaaggaa agcatcaacc aatgttgatt
     3241 ttccagcgtc aacgtgggct ataactgcga tatttataat tttatgtttt tgtgacattt
     3301 ttgttgctcc cttcgattaa aataacttat taattatagc acaatatatt attaggtaca
     3361 aggaggaata tattaaattg tattaatata gtttaaattg aataaaactg ttagttttgc
     3421 atctgaaaat tattttgttt aaaaaaattt tttagacaaa taataacctt aatagataaa
     3481 aagattaata aaagagtaaa ggatgatata tgttggaagt aaccaaaata ataattaaat
     3541 attttgcaag tgaaagtgat acttttgtaa aagaagaagt ttgtaatttt gaagaacttc
     3601 ttaagtggtc tcataaattt attgaaggta aaaagataga agatgttttg tatataaagt
     3661 ctaaagaaaa ctataaagta aatgtgatta agtctttaga aattaaggaa ttggaaatag
     3721 tgagaaaaca acagatacca ccgtacatgt agtattaata atatataaaa ttaatatgta
     3781 atactcgcaa taggatataa atggtatata tggtaataat ttatattaaa aaaactttta
     3841 taaagtttat atttgggtat aaaagtatta attagacatt aattaggagg aacttaaata
     3901 tgataagagt atgtccaaac tgttcaaatg tggatgtaaa taagctaaaa actttggttg
     3961 gagaagaaaa tgtaaagact ggttgtatag gtcagtgtag agcatttaaa aaagaagctg
     4021 ttggaataat agacaatgag cttgaaataa aggaaacaga agcagagttt tttgaagcat
     4081 gtaaagaata aattttaaat aaaatataat aaatagagca tctaaattgt ataagcatta
     4141 aacaatttag atgctctatt tttatatata catttaatta tgagtatact tttctattaa
     4201 atacaaataa atataactaa tacatttatt tatt
//
LOCUS       DAYAOX010000266         4233 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.269, whole genome shotgun sequence.
ACCESSION   DAYAOX010000266 DAYAOX010000000
VERSION     DAYAOX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.269"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(170..370)
                     /locus_tag="ACGI57_003389"
     CDS             complement(170..370)
                     /locus_tag="ACGI57_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9718440.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(372..722)
                     /locus_tag="ACGI57_003390"
     CDS             complement(372..722)
                     /locus_tag="ACGI57_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HFG9718441.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            884..1072
                     /locus_tag="ACGI57_003391"
     CDS             884..1072
                     /locus_tag="ACGI57_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718442.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            complement(1549..1749)
                     /locus_tag="ACGI57_003392"
                     /pseudo
     CDS             complement(1549..1749)
                     /locus_tag="ACGI57_003392"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2108..2596)
                     /locus_tag="ACGI57_003393"
     CDS             complement(2108..2596)
                     /locus_tag="ACGI57_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFG9718443.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            3400..3774
                     /locus_tag="ACGI57_003394"
     CDS             3400..3774
                     /locus_tag="ACGI57_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9718444.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            3879..4233
                     /locus_tag="ACGI57_003395"
                     /pseudo
     CDS             3879..4233
                     /locus_tag="ACGI57_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         1746 a          488 c          503 g         1496 t
ORIGIN      
        1 attaataata caatagaatt agttatcatt caacatatct agttgaataa ttaataaatt
       61 tatatatgaa ctttaaatta tttgaaaaaa taaaaagccc ctctgtcagg agtgacttaa
      121 aaattgaatt taagtttgta tttaaaacac atttttaata atgcaaaaac taatcaaaaa
      181 taaacaattc atcaataggt gtatctacta tttttgatat atctattgct aatttcaaag
      241 aaggattata ctttcctgct tctaaacgca taatggtttc tcttctaact cctacttttt
      301 ctgctaattg ttcttgagtt aattctttta attgtctata tttttttagg ttacaagtga
      361 atttaggcat attatttttc ctctaaaata taaagtttta tagcataaac atttaaagtg
      421 attgcatatg taattgatag taaaacacac attaattcta tattcctaga cactatagta
      481 atacaccata aaatgaataa ttctataata gcaagctgtc ctgtaaatgc catagctgct
      541 ttttcatctt gtacatacct ttcatctgct ttatcaccat ttatttttgc tataataaag
      601 tttgagaaaa aagcaaaaaa tccaatatat gtaaggtcag caacattgcc agatgaaaaa
      661 tatctaagac ttaaaaatcc aaggaaacca caaaaaccca taaaaaattt tttctgaatc
      721 attttttatc ctccttagta attttaaaag agatatattt atatctttat gatacaaata
      781 tatctctttt atattctttt gtcaatagaa aatcagtatt atacaatatt tatactgcac
      841 cataattgtt agagcaaaaa tctaatgctt gagatgttta tgtatgacta aatatgattt
      901 taatttcaaa ttaaaagtag ctaaatttta tttgaataga gaaggaagag atattttaat
      961 agtaaaacaa tatggagttt atgaccattc tcaagttgaa agatgggtta atatattagg
     1021 agagagtgaa ctaaaaataa aaaaattttc tatactatat ggttcaagtt agatgttata
     1081 aactatatga tgataactaa atatagtgta caataaatcg ctaagtattt tggaataaac
     1141 aatagcacag ttatagcaac atgaaaataa aaatttttac atagtggtgt tgatgccttt
     1201 tctaaaatga aaggaagacc ataatgaata aaaacaaaaa cactaaagaa aaaataaatt
     1261 aacaagagaa caagaattag aatatgaaaa taaattttta aaagatgagc ttgagttttt
     1321 aaaaaagatg tgtgtctcag ggaagccaca ttaaaaaaca gtggtatcat tattaatcaa
     1381 aagaaagtta gatgtattat caaaaaatta gaacttaaat gcatagcatt ttttcataaa
     1441 tcacataaat ataactccta taaaggtata attggaaaag taaaaaataa taaaacaaat
     1501 agaagattta aaacatcaat agcatatcag aaaataacaa atgatatgct agaatttata
     1561 aaatatatct aatgataaaa atggaaaatc aattataaaa aaggcatatc tcgattcatt
     1621 ttttgttata tagtcaaata agaatactat agatgaaaga tacgattttg catatgtatt
     1681 aagtaagtat tctgatgttt ttggttgata cacatgcaat aaagaagttg tgtttgcaga
     1741 taaattagca aaactatagt ataagttgca acaataaaga aaatgaaaat tatcatacta
     1801 aagcataagg catgttacaa ttttattaga taggacatta acatacacta aagtactttt
     1861 tattataaag agtacttatt tttttgaaat tcattaatgt ataaactatc aagaatatta
     1921 ctcaacataa tttaaaaaca acttgaatag gtatttttaa attatgtgat ataataaaaa
     1981 atagaagtgt atgccaatac acttctgtag ttataactaa gcattctatt cttgaggagt
     2041 gcttttttca tttttataac caactctttt tactttaaca ataattatca gacctatata
     2101 tttattttta attgtctgat ttattagata aaaaagttat tatcatattt ttatcatttg
     2161 gtatttttat tgaactacca taatcttctt ctaatttatc tagaccactt ttacttattt
     2221 ctccatctat aattatgttt aattctaagt attcttcact accattagat gacactaaag
     2281 tgtacgaacc attataaggc atgcctaaat tttctatttt atgtgccaaa gggtcttcat
     2341 caccaatacc agagtttact gttatttttc catctttttt agtccaccat attatgtttc
     2401 cttcagtata ttttgattca tctgcatagt agctaagaga cgcacttttt attgcactta
     2461 tatcagcttc aagtttagct atctttgctt tttctatatt tttaaataat gctggaagtg
     2521 ctactattgc caatattcct attatagcaa ttactactaa taattccact agagtaaatc
     2581 cttttttatt tttcatttat ttcccctcca tataaaatta ataatatata aatttttatt
     2641 ttgttaattt ctactttcta tattttacca tatatagaca aaaaaatcac tttctatgac
     2701 aaatatacta ttattttaca atttatggaa atgatttaaa atgacatttc ttgttagaaa
     2761 agatataaga aatggattaa aataaagaaa actacagtca tatcaatgtc aataatatta
     2821 tttaaaagca gatgaaaata aaaagaggac tcagaaaata atgtctattt aattatactt
     2881 acttaccaat accataactt gatataaaca agattatact acattttttt atgctctagt
     2941 ataaaatcat ctaactgtgt atctataaaa tcaaaataat tatatgaact atcagtataa
     3001 taagtcaaaa tatgtataca tgaatacata ttttaaaaga tatatattat tacataccta
     3061 aaaaactttc ataaaaacaa aaataaaata tgcttttagt aacttgttaa cataacttat
     3121 aatttatttt ataagcatta gcctatatag ctttgtgcta ctaaatctct ttgattctaa
     3181 cgatttagtg ctattctaca actaaaataa tacaatagag ctatttattg tttggtagca
     3241 ttgtttgtaa attaaacaaa tttatacata tgaatattat tttacattca aaaaaacaga
     3301 ctaaatatgt agaatatttg gtaaaactta cctctagttt ttgtcttaaa aagctcctat
     3361 tattaactta agttagaaga attaaggaag tgacataaat tgaaagaaga aatattaata
     3421 gaaaaattat taagagcaga attaatagta atggagtatt tatggaaaaa agacactcta
     3481 atgcctaaaa aagaaattgt aaaagaaata aaacagattt atggatggca taaaagtaca
     3541 ataaaaatac tactaaaaag gctagttgct aaaaggtatt tggctagata tattataaat
     3601 tctcaatctc attataaaat aatagatagt aaagaatatt atacttttaa gaaaaaggta
     3661 ctaaaatcta gtaaaagcat aaaaataatg cgttcactta ccactacaca taaaaatgta
     3721 agtaaagaga agttagacct attagatgag tattatagaa acttaaaaga atgatatagt
     3781 aaaaatcgaa tgtaattatt taccttaatt cttttgtttg taaaacttta ttgatacaga
     3841 atttaacata tacataaatt ggtaaaggaa tgtatactat gaaaattagt aaattaccag
     3901 aagcggaatt aaaagttatg agatatattt gggaatcaga aaaggtatta atatcaagag
     3961 aaatcaccaa gtctatggaa caaaaatatg aatggaaaga tacaactata tttacagtat
     4021 taaaaagatt actaaagaag agagttttta gaagcggaga aaatagataa gtgtacacac
     4081 tataatgtat tagtaaaaaa aaataatatc agagatttta aacaaaagaa tttttaaaga
     4141 atacacataa aaattcaata ttaagtttaa tgtcagcact atgtagtaag gatgatgaag
     4201 tagatggtga tattttaaat ttattagaag aat
//
LOCUS       DAYAOX010000267         4199 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.270, whole genome shotgun sequence.
ACCESSION   DAYAOX010000267 DAYAOX010000000
VERSION     DAYAOX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.270"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(75..890)
                     /locus_tag="ACGI57_003396"
     CDS             complement(75..890)
                     /locus_tag="ACGI57_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9718445.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            complement(1187..2629)
                     /locus_tag="ACGI57_003397"
     CDS             complement(1187..2629)
                     /locus_tag="ACGI57_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HFG9718446.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            complement(2756..3142)
                     /locus_tag="ACGI57_003398"
     CDS             complement(2756..3142)
                     /locus_tag="ACGI57_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HFG9718447.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            complement(3179..3568)
                     /locus_tag="ACGI57_003399"
     CDS             complement(3179..3568)
                     /locus_tag="ACGI57_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HFG9718448.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            complement(3600..4136)
                     /gene="rbr"
                     /locus_tag="ACGI57_003400"
     CDS             complement(3600..4136)
                     /gene="rbr"
                     /locus_tag="ACGI57_003400"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HFG9718449.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
BASE COUNT         1330 a          813 c          445 g         1611 t
ORIGIN      
        1 acgtaacaaa aaagatattt tctatagaaa atatcttttt aaccttcaac ttaaatctta
       61 tatactttca aatactatat acttatggtc agccctactc caacttcatt tatattcaat
      121 atttcattca tcttcgcttt taccttaaaa gaaacacagt gacaagggta caacattctc
      181 atttcttctt tatccaaata ttgtattgta ctatctaacc tattattcaa ttcaaaaaga
      241 tgaaaaccac ctagtatacc aataactcta tcatctccac ataccttttt agcatactct
      301 actatattac aaattccact atgagaacat cctgtaacaa taaaaatacc tttatcagtt
      361 ttacaaacta tagcagaatc atctaaaaca taatcatctt cccacaaatc tcctatttta
      421 catttaccaa ttttagctct tttttcaaaa tcatttatag aaggtatttc acctaaaaac
      481 acacaattct tacttagatt aaatggctta tccttaggat tatacataaa aatattttta
      541 atttcctcag cagaaaatgg agctcctatg cttttctctc catgttttct aggtataaaa
      601 cagttaggat gtgaaataag ttcaactgta gataaatcat acctattttc tagaaactgg
      661 atacctcttg tatggtcatt atgcccatga gaaaaaacaa catgagttaa atttcctaaa
      721 tctatattta atttttcagc gtttgatatg aatacatctg aataccctga gtcaaacaaa
      781 atcctatttc catcaatctc aatataatag cttactgctg gttctccaac aaagtatcta
      841 tcaatataag tattattgtc aactaaaact tgtatcttca atctttccat aataatcact
      901 ccttaaattt ttatttactt taaagtacag aatctttagt ttaatagtac gacatcacag
      961 aatattttac aatttaatat ctttaatttt cataaataga aggagtctat ctcaaaataa
     1021 agagataaga ttctttttca ttgctaaaat gataaatata tttggcttca taaaaaaaag
     1081 agtgacttat gaactaaaaa gttcatttta agacactctc ttttataaaa tatatttatc
     1141 atttactttt atagctatct aaaatctaat agtttcaaac ttcaatctat acatgctgta
     1201 tgtttgtata atttgatatt tcagagttaa cagtatataa atcatcaaca cttaaatcat
     1261 gtatatcttt atgaccagaa attctagcaa aagtcttaag ttcttctaaa cttacattta
     1321 aaaagtttgc cactctatta gcactgtttt ctatatgtaa tctttttctc aattcctcat
     1381 cctgagtagc tactcctaca ggacacttac cagaaccaca tatacgatat tgttgacacg
     1441 ccgctgccat aagagcagaa cttgcaatag caactgcatc agcacccata gcaattgcct
     1501 tagcaaaatc agtagatata cgaagcccac ctgttataac caaatctata tctaatccat
     1561 gtgtatctat gtattttcta gcacgataaa gagcaaatat tgttggaatt gaagttgcat
     1621 ctttaagcaa ctttggactt gctccagtag ctccaccacg accatcaata gttacaaaat
     1681 caggtttagc ataagctata aattccatat ctttttcaat atgacctgct gaaattttaa
     1741 cacctattgg acgcccttct gaaacttctc taagttcatc tacaagtttc tttaaatctt
     1801 ctttggattg tatttcctca aaacaagatg gacttattac atcttgacct acaggcttat
     1861 ttcttacttt tgctatttct tcagttactt tttcgccagg aagatgacct cccataccag
     1921 gttttgttcc ttgaccaatt tttatctcta tagcatcaga atttttaaga ttttcttcag
     1981 ttacactata tttatttggt acatactcaa aaatatactt gtatgaagct tccttctctt
     2041 ctggtaatat acctccttca ccactacaca tagctgtctt attttgggct gcaccctttg
     2101 ctaaagctat ttttagttct ttagacaaag ctccaaatga catgtgggaa atataaactg
     2161 gattttctat aatcataggt ttctttgctt tttttcctat tattgttgtt gtatttactt
     2221 catcatgctc atttaaaggt aatggattta attgagcacc cataattaaa atatcatccc
     2281 aagaaattac tggtagttta gttctcatag gttctattat agattttcct gtaacagcca
     2341 tttcgtgaat tatatccata tgtttctcaa cttcatcatc tcttcttaaa tatgtactta
     2401 agtaatttaa atctccctct ttttcagtat ttaatttaga ttcatcttca actatgtctt
     2461 ctgctatctt ttcatcttct tcatagtaaa cagtacttat ctttccaaaa taactacgac
     2521 cttttgtaca tacagggcat tcccaatctt cacttaattc atcccatgtt ttatcattct
     2581 tagattcatc ataaatatat ccacaaacac tacatttata aattgacata ttattttctc
     2641 cttatattat attgacatta aacaaaatta aaacttgaat ttatataaat ttataggagg
     2701 ctatttgtat gaaaatacat ttatagcctc ctttttgact caatttattt aaatattaca
     2761 gttcagcctt ccataatcca tgtaaattac agtaagcata ggcagaaatc actttatcat
     2821 catcattcaa agcaaattta actacaggtt ctccatctac tgctagacat ttgcgttgac
     2881 caccttgttc agtcataaga tacacccatg ctatatgatg ttctttagtc attggatgag
     2941 ttgtgctact tactttaaca gtaacattat ttccatccac ttcaattaca ggtacatgtt
     3001 tttcaactgc tgcatctgtt gtattaggaa caagttcctt cataggtttt ccacaacaga
     3061 aaataggtac accaccactt tgtatcattc ctacaaggtt tccacaagtc tcacatataa
     3121 aaaatttttg ttcactacac ataaactcac ctcattaatt atttttagaa tttgaaaact
     3181 aacctacttg gctacacctt ttacaattat tacaaatccc tttaaaactt atgttataag
     3241 atgttatttt tatgtctgtc tcctcagata ttaattcttg taaattatta agaacttcgg
     3301 attctaaatc gtatacctct ccacatactt cacatatgat atgatagtga ttatctattc
     3361 gaccatcaaa tctatctgga taccctggaa tactaacctt tcttataaag ccatgttccg
     3421 ttagctgagc taaatttcta tacacagttc ctaagcttaa atttggatta tcttttttta
     3481 agttttcata taggtaatcc gcagtaggat gaaccggatt atttaatatt tcatttaaaa
     3541 tcagttctcg ttgtttagaa aatttcatag ttaacattcc tttttaataa tgataatttt
     3601 taataatttt cagcttttat attgaaatac gcttttggat ggtcacatac tgggcatttt
     3661 tctggtgctt cagtacctac atacacatga ccacagtttt gacattgcca aacaacttct
     3721 tcatctcttt taaatatttt tccttcattt aagttttcta aaagctttaa gtatctttct
     3781 tcatgctctt tctctatttt tccaacagct tcaaataaat aagctatctt gtcaaatcct
     3841 tcttctctag cttctttagc aaattttgca tacatatcag tccattcata gttttcacct
     3901 gctgctgcat cctttaaatt ttcctctgtt gaaggcatac catcatgtaa aagtttaaac
     3961 cataatttag catgttcctt ttcattgtta gcagtttctt caaatatagc agctatttga
     4021 ttataacctt cttttcttgc ttttgaagcg tagtatgtat atttatttct tgcttctgac
     4081 tctcctgcaa atcctgccat taaattcttt tctgttttag ttccttttaa gttcataatt
     4141 aattcctccc tttttaataa tagtaatgat tactatttct atatttatat attatctca
//
LOCUS       DAYAOX010000268         4157 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.271, whole genome shotgun sequence.
ACCESSION   DAYAOX010000268 DAYAOX010000000
VERSION     DAYAOX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.271"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            61..1755
                     /locus_tag="ACGI57_003401"
     CDS             61..1755
                     /locus_tag="ACGI57_003401"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFG9718450.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYIRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2087..2197
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2301..2672
                     /locus_tag="ACGI57_003402"
     CDS             2301..2672
                     /locus_tag="ACGI57_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HFG9718451.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2767..3714
                     /gene="trxB"
                     /locus_tag="ACGI57_003403"
     CDS             2767..3714
                     /gene="trxB"
                     /locus_tag="ACGI57_003403"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFG9718452.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3818..4135
                     /locus_tag="ACGI57_003404"
     CDS             3818..4135
                     /locus_tag="ACGI57_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HFG9718453.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT         1726 a          387 c          776 g         1268 t
ORIGIN      
        1 agtacatata aatctatatt tataaataca ataaagtaaa gttgtaaagg agtgctttaa
       61 atgaagtttt cagagtttaa gtatgaaaga cccaattata atagtatgaa aaaagagttt
      121 ttgagttgtg tagaagatat aaataattct cgtaattata aagagcaaca aaaaaatata
      181 cataagatta atttattaag aaataaaata gaaacgttgt caaatatagc ttcgataaga
      241 tatagtacag acacatttaa taaattctac aaagaagaaa aaaattattg ggatgaatat
      301 atgcctttgt atgaagaatt aaattcatat ttttataatg ccattgtaaa ttcaaaattt
      361 aaatatgatt tgataaaaga gtttggagag caatttttca caatagtaga gtattcttta
      421 aaaagttttt ccaaagaaat aataagtgaa ttgcaggaag aaaataaatt gtgttcagag
      481 tatactagac ttttagcttc agctgagata atgtttgatg ggaaaataag aaatttatct
      541 ggtatgggga aattcatgta ttctaagaat agaaaaacaa gagaattagc aaacaaagca
      601 tattataatt tctttgaaga aaatgaaact aaatttgatg atatatttga taaattggtt
      661 aaacttagag ataagatgtc taaaaaatta ggttttgaag attttgtaga gcttgggtac
      721 attagaatga tgagaagtga ctacagagaa tatatgataa aaaatgttag aaaacaagta
      781 ctaaaatatg ttgttccaat ggcaaatgag ctttatgaaa aacaagctaa aagaattggt
      841 ctagaatatt taagttacat agatgaaggc gtggaatttt taacaggaaa tgcaagttta
      901 aagggagatt caagatacat aattaaaaat ggtaaaagga tgtattctga actgtcaaaa
      961 gaaacaaatg aattttttga ttttatgtta gaaaatgaac ttatggattt agaaacgaag
     1021 aaaggaaaag gagctggagg gtattgtact tatataccag attataagtc accattcatt
     1081 ttttctaatt ttaatcaaac tgctgatgat atagatgtat taacacatga ggcaggacat
     1141 gcatttcagt tgtatatgtc tagatggatt gatatgccag aaattaattt tccaacgctt
     1201 gatagttgtg aaatccattc aatgagtatg gaatttataa catggccgtg gatggattta
     1261 ttttttaaag aagatacaga taagtacaaa ttcactcact tatcatcttc aattaaattt
     1321 ataccttatg gagtaattgt tgatgaattt caacactata tttacaaaaa tcctaatgta
     1381 gataagtcaa aacgaaaaga aatctggaga tttttagaga aaaaatactt acctcataga
     1441 aaatatggtg ataattcttt tttagagaga ggttgttggt ggtttaaaca aggacatata
     1501 tttaaaaatc cgttttatta tatagattat gtattagctc aaatttgtgc gttacaattt
     1561 tggaaaaaaa tgattcaaga tagagatgca ggatggaaag attatataaa tatctgtaaa
     1621 gttggaggaa caaaatcatt tttagatatt gtgagtatgg gaaatttgta ttctccattt
     1681 gatgatggct gtattgagtc tattataggt gatgttaaat cttggtttga tgaaataaat
     1741 gacagcaaat tataaaaaat tatgagaatt ttattgtata aaagtaaaaa attaaaaaat
     1801 atataaaaag tgattatttg ttgtgaaaat agtcttgttt ttaactaaat tgataaaata
     1861 aactaaaaaa gatttgtact taaaaaaaaa agaaaataca agaaatatgc aatatcaaca
     1921 tttggaaaaa aaagtaaaaa tgtatacaaa atacatataa aaaaactatt gcaaatttta
     1981 tagaatgttt atatacttaa gtcaacagag atagaaaaaa gctatttata gaaaacaaag
     2041 gataaattgc ttaattttaa attttctgaa aaaaataata atccggatga agagtatggg
     2101 agactgcttt ataaaaagcg accgaaggga caagtttaat cttacttacc caaaagatta
     2161 gatgaaattc tcaggtaaag gtgactgtac ttggacgggg ctctgaaaat gattttaaca
     2221 gggaagacaa attgggagat ttgtttttcc tgttttttat ttatcaagta ataaataaaa
     2281 gtttgaaaga gagtaaaaaa atgataataa ttacgaataa tccaaaagta aaagaagaag
     2341 ttcaaggcag agaagttttg tttaaagaca caacttatat tggaatctta gaagcaagta
     2401 gagatttgat acatgaaggt tatgaacttt tatctcatcc actctatggg agtgtaaagc
     2461 caaatgaaac accttacagg actgttatac taaagaaagg aaatcgtttg gatataaact
     2521 ctttaacctt aattgaagaa gcaatcatca ctgcaagtaa atttcagaat aataaaaaga
     2581 ctccaaaatg gacagaaagt gttcaagatg attttagagt aattgattat gatatatttt
     2641 ataatacaat acaaagaatg cagtatgaat aaaaaaattg ttaaaaagtt attaaaacag
     2701 cctaaatatt aaatctaatt taatattaaa taaaatgaga aatttaattg gggaggataa
     2761 gtcaaaatgg aaaatgtgta tgacttagta ataattggtt caggaccagc agggcttgca
     2821 gcaggtcttt atggagcaag agcaaagtta aaaactctaa tcctagaaaa agacaagact
     2881 ggtggacaaa ttgtaataac acatgaaatt gcaaattatc cagggtcagt tccaaatgca
     2941 acaggaccaa gtttaatagc tagaatggtt gaacagtgta aagagtttgg agcagaaatg
     3001 ctaagagaca acattgttga tactgagcta gatggagata taaaagtttt aaaaggggaa
     3061 aaggcagaat atagagcaaa agcagtaata ataggaacag gagcaacacc aagaaagatt
     3121 ggttgtcctg gagaaaaaga acttactggt aaaggggttt catattgtgc aacttgtgat
     3181 gcagatttct ttgaagattt tgaagtattt gttgtaggtg gaggagacag tgcattagaa
     3241 gaagcaatgt atttaactaa atttgctaga aaagtaacaa ttgtacatag aagacaagga
     3301 tttagatgtg ctaagagtgt tgaagaaaaa gcaaaagcta atccaaagat agagttttta
     3361 ttagatacag taattgaaga aatcaaaggt gatggaatat tagaatcagt tgtatttaaa
     3421 aataaagtta ctggagaaac tcatgaatac tttgcagatg aagaagatgg aacaatggga
     3481 gtatttgtgt tcgttggatt agatgctcaa actgatttat tcaaaggtaa agttgatatg
     3541 gatgaaaaag gttacataat aactgatgaa gatatgagaa ctaacatacc aggcgtattt
     3601 gcagcaggag attgtagatc taagactcta agacaggttg taactgcaac taatgatgga
     3661 gcaattgcat ctatagtagc tgagaaatac attgatgaaa aatttgggaa ctagaatctg
     3721 taaattaaga aatagtgtct gtaaatttaa tgaataatat ttaaaattaa aattaatatt
     3781 taaaataaaa ataaaaattt caagggggaa taagaaaatg ttagatttag ataaggctac
     3841 atttgaagaa gaagttttaa atgcagaagg ttttgtattt gttgattttt ggagtgaagg
     3901 ttgtgagcct tgtaaagctt taatgccaga tgttcataaa ttagctgaga cttatgggga
     3961 taaaataaaa ttctgtaaga tggatacaac aaaagctaga agattagcta taaaacaaaa
     4021 agtattaggt cttccaacta tggctatata caaagatgga gaaaaagtag acgaagttac
     4081 taaggatgat gctacagttc ctaatataga aaacatgatt aaaaaatatc tttaatatgt
     4141 tgataaatta ttaacat
//
LOCUS       DAYAOX010000269         4145 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.272, whole genome shotgun sequence.
ACCESSION   DAYAOX010000269 DAYAOX010000000
VERSION     DAYAOX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.272"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(155..1252)
                     /gene="ychF"
                     /locus_tag="ACGI57_003405"
     CDS             complement(155..1252)
                     /gene="ychF"
                     /locus_tag="ACGI57_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFG9718454.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            1568..2833
                     /locus_tag="ACGI57_003406"
     CDS             1568..2833
                     /locus_tag="ACGI57_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFG9718455.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            2848..4134
                     /locus_tag="ACGI57_003407"
     CDS             2848..4134
                     /locus_tag="ACGI57_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFG9718456.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
BASE COUNT         1590 a          467 c          652 g         1436 t
ORIGIN      
        1 aaagagtttt tcataataat ttatacaaat catttgaaat actcttcttt tatttttaaa
       61 ttctatataa ttattataag ataatattgt attattataa tcttagtttt ttagttatta
      121 tatataatag tcaaagtgta tacgattaat cattttaaac attaaatcta aataatatta
      181 catctccatc tttaactatg tattctttac cttctaattt aaccaaccct ttttctttag
      241 cagcagccat agtaccatgc tcaaccaaat cattaaaggc tattgtttct gctctgataa
      301 atcctctttc tatatcagaa tgtatttttc ctcctgcctg aggagcttta cttccaactt
      361 ttatagtcca tgctctaact tcttttggac cagcagttaa gaatgaaatt aaaccaagta
      421 aagcataaga agattttata agcttatcaa gccctgattg ttcaagacct aatgtttcta
      481 aaaactcttt cttttcttca gcagaatcaa gttcagaaat ttctgcttct atttgtgcac
      541 atactacaac aacttcggca tcttctgttt ctgcaaatgc tttaacttgt tgaacatatt
      601 tattattttc tccattatca gctaaatcat cttctgatac atttgaagca tatattacag
      661 gcttagatgt aagtaaatcc aatgaattta cgaatgtttg ttcatcttct gtaaattcca
      721 tagttcttac acatttactt tcttctaaag tagacattat tgattttaat aaatcaagtt
      781 cagaacctaa agttttatca gccttagcag ctttttgagt tttattaatt cttctttcaa
      841 gaatctctat atctgagaaa attaattcta agtttatagt ttctatatca cgaagtgggt
      901 ctacagaacc atctacatgt acaacatttt catcctcaaa acatctaaca acatgaacta
      961 ttgcatcaac ttctcttata tgtgataaga atttatttcc taaaccttct cctttagaag
     1021 ctcctctaac taaacctgct atatcacaaa attctatagc agtaggaact attttttcag
     1081 aattatataa ttcttgtagt ttgtttaatc tttcatctgg aacagataca actcccacat
     1141 ttgggtctat tgtacaaaaa gggtaatttg cagattctgc acctgcttga gttattgcgt
     1201 taaatagtgt gcttttacct acatttggta aaccaactat acctaatttc atattaatcg
     1261 tcctctctct tattcttttt tatattcttt atataacttt aaacaatcaa tctataggat
     1321 tgcaaaattg ttttaattat agacatacat attgattata catcatatat atcaagcttg
     1381 taaactagac tttaaagcat attgatttaa ataatatttg tatttaattt aaataaaaat
     1441 attgctttta gaaaataata aaaaggatat aattaaatag aaggttttta tatgtgagta
     1501 tatgtaatta aaaaataaat aaatttcaat tttaaaattg atgtttaaat aaaaatagga
     1561 ggtacatatg gttaatacta aaaaaataat aaatttaggt aataatatta atcttacttt
     1621 gataaaaaca gaaaagttta aatcgaatct tataagttta tatgttcaga gactattgga
     1681 tgaaaaagaa actacaaaaa atgctctaat accatcaata atagcaagtg gaagcgctaa
     1741 atatcctagt gctagagcaa tatccaataa attagatgat ttatatggtt catctatggg
     1801 agctgatgct gtaaaacgtg gagaacgtca agtattgagt tttaaagtaa ttaacataag
     1861 tgaaaagtat ttagatgaga gtatatttga agaagtagta gaatttttta atgaagtaat
     1921 aaataatacg cttgttgtag atggtgggtt taaagaagaa tatttaaata ttgaaaaaga
     1981 gaatttaaga gaaaagatac aatcaataat aaatgataaa aaggaatatg cgcaagataa
     2041 atgtattgag gctatgtgta aaggagagag atatagtgta agtgaatttg gttatgaaga
     2101 tgaaatagat agtataactt caagagagtt atatgaacat tataaaaaca tacttaaaac
     2161 atcacctata gatatagttg tagaaggtaa ttttgatgaa gataaagttg tagatataat
     2221 aagtaaaaat ctaaaatttg aaagagaaga aattataaat ataccaagag cagattttat
     2281 aaaaaatgtg gatgaggtaa aagttataga tgaacaaatg gaaataactc aaggtaagtt
     2341 agttatgggt tatagagcaa atgtggatta tgcagacata gataaatatt atgctttagt
     2401 agttgggagc aatgtcttag gtggtggtcc acactctaaa ttatttgtta atgtaagaga
     2461 aaaagaaagt ttatgttact atatattctc ttcaatagag aagtataaaa gtatcatgtt
     2521 tatatcatct ggtatagaaa ctaaagatta tgataaagct gtagaattga taaaaaaaca
     2581 agtagaaagt gtaaaagcag gtgatatatc tgatgaagaa ttagaaaaca gtaaacttgc
     2641 tttagttaat tctatgaaat ctataactga taatataggt ggtatgtctg attttgtttt
     2701 ctctcagtct atggcaaaga caaattctga agtacaagac ataatatcta gtatagaaaa
     2761 ggttacaaaa aaagatatag ttgaagctat aaaaaatata gagcttgata ctgtatactt
     2821 cttgagaaat taatggagga gatgtctatg gaaaaaatag ttaatgatat attgaaagaa
     2881 gaagtttatt atgagaaatt gcaaaatgga ttagatgtat attttatgcc taaaaggggt
     2941 tttatgaaaa agtatgctat tttggctact aattatggtt caaatgactt agaatttgta
     3001 ccaattggag aagataagaa aattagagtg aatgaaggta ttgcacattt tctagagcat
     3061 aagatgtttg agcaacctga tggaggagat gcatttgata aattttcaaa attaggagta
     3121 aatgcaaatg catttactaa ttttacaatg acagcctact tattttctgc tactgaaaat
     3181 ttttatgaga gtttagaaca tctaattgac tatgttcaaa caccttattt tacagatgaa
     3241 aatgttgaaa aggaaaaagg tataatagca caagagataa aaatgtacaa tgatgaccct
     3301 gattggaatg tatactttaa ttgcttgaag gcaatgtatg taaattatcc tgctagaatt
     3361 gatatagcag gtactgttga tagtatatat aagataacta aggaagagct atataaatgt
     3421 tataatactt tctataatcc aggaaatatg gctttatttg ttgttggtga cttagatgta
     3481 gaaaaagtta tagatgtaac taaaaagtca aacaattaca aagtagacag actctctaag
     3541 agtatagaaa gattttatcc agaagaacca gaaagtgtaa aagaaaaaga ggttatagaa
     3601 aaattcccta tatctatgcc tatgttcaac ataggtttta aagatagcaa tgttggttta
     3661 aaagggaaag aacttttacg taaagagatt gtaactgata tacttgtagg gatgttattt
     3721 aaaaaaggaa gtaagcttta tgaagatttg tatatgcaag gtttaataaa tgaaaatttt
     3781 ggagctggat tttcgtctca agtggattat gcttttagca taatagctgg agattctaaa
     3841 gagcctaaaa aagtaaaaga aataattctt gattatatag aaaaatcaaa aaaagaagga
     3901 ttatcaaaag aagagtttga gagaactaaa aagaaaaaaa tagggtcatt tattaagtgc
     3961 tttgattcta taaactttat aggtaatagt tttatatctt atgtttttaa ggatataaat
     4021 ctattggatt atttagatat tattaaagat attacttttg aggaagttga agaaagatta
     4081 aaagagcatt ttaaagaaga gtattgtgtt atttctattg tagagcctaa atagaaactt
     4141 aatca
//
LOCUS       DAYAOX010000270         4140 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.273, whole genome shotgun sequence.
ACCESSION   DAYAOX010000270 DAYAOX010000000
VERSION     DAYAOX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.273"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            249..1652
                     /locus_tag="ACGI57_003408"
     CDS             249..1652
                     /locus_tag="ACGI57_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9718457.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1743..1983
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2026..2724
                     /locus_tag="ACGI57_003409"
     CDS             2026..2724
                     /locus_tag="ACGI57_003409"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFG9718458.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2747..3748
                     /gene="trpS"
                     /locus_tag="ACGI57_003410"
     CDS             2747..3748
                     /gene="trpS"
                     /locus_tag="ACGI57_003410"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFG9718459.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1559 a          457 c          738 g         1386 t
ORIGIN      
        1 taaaatttta taataaaata atattgaaac aattttaaaa atttagtatc attagaatta
       61 aagtggtaaa acttttaaat ttatcatttt taataagatt tgatttcaaa gtatttaagg
      121 tttattatta aaactgtttt tgaatatgca aatattttga aaaatatcag tttaataata
      181 atgaatctat tttattaatt ttgaaattaa atttaaaata attaatagag aggggaaatt
      241 aaattaggat ggaaaagaaa aaaataggcc tatggagttt ggtatttatg aatgtatcag
      301 ctttatatgg tataagatgg atagctaaat ctacagcatc aagctttggt ttaggattag
      361 gtgcaatacc tatgtggtta ttattctcat ttatatactt tgtaccagga gctttaattt
      421 gtgctgaatt agcttctaca tatcaaaaag gtgatggagg tctttacgat tgggttaagg
      481 aagcctttgg agaaaagtat ggatttttag tttcttggtt aaactggaca gctaaaatat
      541 tttggtattc ttcattttta actttcttag cagtaaatgt ttcttataca ataggaaatc
      601 cagcccttgc tgataataag atgtttgtac ttatattatc tttaataata ttctgggcat
      661 tatctcttat agcaactaaa ggaataagtt ttacaaaaat atttactaat acaggtgcat
      721 taggttcaac aataccaagt gctatactta taatattttc atttgtagca gtatttgtgt
      781 tgaaaaaaca tgatatagct tctacataca caatagcttc aatgactcca aaacttaata
      841 tggatgcatt tgttgctata tcagctataa tgtttggttt agcaggagca gagactgctg
      901 ctaacttcat aacagaaata gataagcctg aaaagaactt ccctaaggca atattaatat
      961 cagctggaat agtagcttca ttatatgtat taggttcaat tgcaataaca atgataatac
     1021 caccagacca aattactgct tctaaaggtg tattagatgc actttctgct gtttgtgcgt
     1081 ctttaggaat tggttcttgg ttggttcaat taatagcttt tggtatagct ttttcagtat
     1141 taggagcaat agttctttac atagcatcac caataaaaat gttatttggt agtgttaaaa
     1201 aaggaatatt tccagatagt ttagtagagg taaatgaaca caagatacct tctaaagcag
     1261 ttatacttca agcaataata gttactataa tactattggt gactacatta atgccttctg
     1321 ttgatgctat atacaatgtt cttgtaacta tgacagcatt aactgcatta ttcccatatg
     1381 tattgttata tgcttcttat attaaactaa gaaaagagag accagatgaa attagacctt
     1441 atactatggc aaaatcaaca agtacttgcc taggtttagc aaaaatggtt ttagttgtaa
     1501 ctgtagtggg tatattatta tctgcagcac cagttatgcc tacatttgct gaaaatgtta
     1561 tatatgagat agagatgatt ggtggaggtc ttctagttat attatctggt ctatggttat
     1621 ggaatagata tgaaaagaaa acagctaaat aaaatgtatt ttaaaaattt cttttggata
     1681 ttataatata tagttgacaa tttatagtgt aaggaatatt ataaaataaa gaaattaata
     1741 aattaaatcc tgagataaga aatagtaaat aaatatattt ttgcacagag aattaaggat
     1801 gctgagaact taataaaaag tatttattga atgggtcttt gaggagtaaa atgaaatgta
     1861 atttaatatt acagagtatt tgatatcgtg tatgcacgtt atagcatata ttaagatata
     1921 tatattcaat tttgagtgta tataaattta ggtggtacca cggaaaatat atccgtccta
     1981 ttgatttagg acggattttt taatatagaa aatttaggag gaaaaatgaa cgtaatagga
     2041 attataggtg caatggatga agaagttagt attttagttg atttaatgga tattagagag
     2101 actataaaaa aagctagctt agaattttat aagggaattt tggaaggtaa gaatgtagtg
     2161 ttagtaaagt gtggcatagg taaggttaac tctgctcttt gtgcccagat actaataagc
     2221 gaatttaagg ttgatgctat tgttaataca ggtgttgcag gagctttaaa cgaaaaactt
     2281 gatgttaatg atatagtaat atcaactgat gctatacagt atgatgtaga cactacagca
     2341 tttggagacc ctaagggagt tataccaaga atgaagactt ctgtatttaa agcagatgaa
     2401 agacttatag atgctgctta caaatcttct gtggaagaag taaagactca taaagtatta
     2461 aaaggaagag tagttacagg agataaattt ataaattcta aagaattaaa agaagaatta
     2521 gtaaatgatt ttggtggata ttgtggagaa atggaaggtg gagctatagc tcatgtatgc
     2581 tatttaaata atactccatt tgtaataata agagcaatgt ctgataaggc agatggaagt
     2641 gctgatgtta cttatgatgt atttgtacat gatgctgcca ataactctaa agacatagtt
     2701 ttaaatatgt taaaatcaat ataaatatat aaggaggtac aaaaaaatga gtgaaaaaaa
     2761 agttatattt agtggtgcac agccatcagg caaaatgact ttaggaaatt atttaggagc
     2821 tataaaaaac tggactgaac ttcaagacaa ttatgattgt tattatagta tagttgattt
     2881 gcatgcaata acagtacctc aagaccctaa agttttaaga gccaatacta tagaattact
     2941 tgctcaatat atagcttgtg gattagaccc agaaaaaaat actatattta tacaatcaca
     3001 tgtcaaagag catgttgaac ttatgtgggt attgaatact atgacttata tgggtgaatt
     3061 aagtagaatg actcaattca aagataaatc acaaaagagt gaagcaaatc taaatgcagg
     3121 tttattcaca tatccagttc ttatggctgc tgacatattg ttgtatcaaa ctgacctagt
     3181 tccagttgga gatgaccaaa aacaacattt agaattagct cgtgatttag caaatagatt
     3241 taataataga ttttcaccta cttttgtagt tccagaagga tactacccaa aaggtggagc
     3301 aagagtgatg agtttgcaag agccaactaa aaaaatgagt aaatccgatt caaatgaaaa
     3361 tgcatttata ctattagcag atgattcaga ttctataaag agaaagataa aaagaagtgt
     3421 tactgattct ttaggtgttg taaaatacaa tgatgaacaa ccaggtatta aaaatctaat
     3481 tgatatatat tctaatttaa gtaaaaagag tgtagaagag attgtaaaca tgtatgaagg
     3541 aaagggttat ggcatattta aagaagatgt ggctgaagta gtatcagaag ctttaagacc
     3601 tataagagaa aaatatgttg accttcttaa taataaagat tatttagaaa aaatatatag
     3661 tatgggagca gaaaaagccg aaaaacaagc tagaaaaact cttagaaaag tttataaaaa
     3721 agttggattt atagagagaa gatactaatt attagctatc tttacttgat aaataaattt
     3781 taaagttatt aaaaaggtat atgatatgaa agagagagtc tcaaagtgaa cttttgttca
     3841 tgaggcactc ttttttgtat gaaataaaat atattttaat catttcaaca atgaaaaaga
     3901 ggattatctc tattttgaga cagcatcttt tgataataaa aaaagatgag cttttgctca
     3961 tctcaaaggg gttagattat tcttactttg gggaaagtag aataaaggta aaagttaact
     4021 gatgtcaact ttgtcttttt tgcatatcac tactgagcat aaaaaaatca gaaaaccata
     4081 gccttctgaa acaacttcta acatacattg tattttatac ccaatatgta atttatgagt
//
LOCUS       DAYAOX010000271         4101 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.274, whole genome shotgun sequence.
ACCESSION   DAYAOX010000271 DAYAOX010000000
VERSION     DAYAOX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.274"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..2321)
                     /gene="carB"
                     /locus_tag="ACGI57_003411"
     CDS             complement(<1..2321)
                     /gene="carB"
                     /locus_tag="ACGI57_003411"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HFG9718460.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIM"
     gene            complement(2514..3563)
                     /gene="carA"
                     /locus_tag="ACGI57_003412"
     CDS             complement(2514..3563)
                     /gene="carA"
                     /locus_tag="ACGI57_003412"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFG9718461.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(3820..>4101)
                     /locus_tag="ACGI57_003413"
     CDS             complement(3820..>4101)
                     /locus_tag="ACGI57_003413"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718462.1"
                     /translation="NSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKIRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
BASE COUNT         1177 a          811 c          415 g         1698 t
ORIGIN      
        1 tccattatac caggtatcaa tatatcttct ttatcacata tagcatctac ttctatttct
       61 ctaccagtta gatatttatc tataagtact ggatttttat ggtctctatc aaatgcatca
      121 tccaaatact gaattagttt ttcttcgtta taagttattt ccataccttg accaccaagt
      181 acataagatg gtctaactaa tactggataa cctaactcct tagcaatttc aacaccttca
      241 ttagttgtcc atacaccttt tccttttggt ctatttatat ctagtctctc tagtaaatca
      301 tcaaattttt ctctatcttc tgctgcatct atatctctaa agtctgttcc cagtataggt
      361 atattttttt catgtaaaaa cttagccaat tttatagctg tttgtccacc aaactgtagt
      421 attacacctt ctggtttttc tttttctatt atatttaaaa cttcttcttc tgttaatggt
      481 tcaaaataca acttatcaga tgtatcaaaa tccgtactta cagtttcagg gttattgttt
      541 acaattatag tttctattcc catttttctt aaagatttta cacagtgaac tgaacaatag
      601 tcaaattcta ttccttgacc tattcttatt ggaccagaac ccaatactac aacttttctc
      661 ttatcactaa ctactacctc atcatattgt tcataagttg aatagtaata aggtgataga
      721 gcttcaaatt ctcctccaca tgtatcaacc attttataaa ctggttgtat attatataaa
      781 cttctcaatt catataattt ttctgggctt atcttcatta agtcagatat acctttatca
      841 gaaaatccct ttttctttaa ttcatgtaaa tattctttat ctaagtcttc tatttttaga
      901 acttttaact tttcttcttg ttctactatc catttaaatt tatttataaa ccacttatca
      961 acaccagtta tttctgatat catttctact ttatagcctc ttcttatcat ttcagctaag
     1021 tcaaatagtc tctcatcatc aggtactact actctagcct ttaattcttc taatgttcta
     1081 ccttctgatg gagcatgaac caatgaatat tttcctgttt caagtgatct tataccttta
     1141 agaatagcag cctcaaagtt acttcctata ctcatgattt caccagttgc catcatctta
     1201 gttcctaatt ttctattggc atgtctaaat ttatcaaatg gccactttgg tatttttact
     1261 accacataat ctaatgttgg ctcaaaacaa gcataagttt ttttagttac tgcattttct
     1321 atctcatcca aagtataacc taaagctatc ttagctgcta cttttgctat tgggtatcct
     1381 gtagcttttg atgctagggc tgatgaccta ctaactcttg ggtttatttc tattatcgca
     1441 tattctaaac tatgtgggtt taaagctatt tgaacgttac atccaccttg aacctctatt
     1501 gcatttatta tgtctattga tgcctttcta agtagttgat actcttcatc acttagagtt
     1561 tgacttggag caacaactat actgtctccc gtatgaactc caactgggtc tacattttcc
     1621 atattacata cagttataca gtttccattt ccatctctca ttacttcata ttctatttct
     1681 ttccaacctt ttatactctt ttcaagaaga acttgtccaa caggacttag ctgtagtcca
     1741 tgtgaaagta tttctcttaa ttcttcctca gtatcagcta tacctccacc agttccacct
     1801 agcgtatatg ctggtcttac aactactgga tatcctatct tattggcaaa ctcaagacct
     1861 gcttctaaat ttgtaactat atcactaact attactggtt gatttatttc tttcattact
     1921 tctctaaata aatctctatc ttcgcccttt tttatagatt ctatagatgt tcctataact
     1981 tttacattat atttatctaa tataccagca tcatgtaatt ctacagctaa gtttaatcct
     2041 gtctgaccac ccatacctgc aagtaaactg tctggtcttt ctttttctat tattttttct
     2101 ataaattcta ttgttaatgg ttctatatat attttatctg caatttcttt atcagtcatt
     2161 atagtagctg ggttactatt tactaataca acttcaattc cctcttcttt taatgcttgg
     2221 caggcctgtg ttcctgaata gtcaaactct gcagcttgac ctattattat tggccctgac
     2281 cctaatacta aagttttctt tatactatct aatttaggca taactctttc tccttatcta
     2341 aacaattttc acagttctta tcttcatcat ctatacaatc aactttaatt actttatttt
     2401 atcttaagtt caaatttact catcaataca tctatttaat acatctatgt taattaaacc
     2461 aaactttttg atttaagtta ttcttgtttt ttagttagct attctaatgt tgtttatttt
     2521 actaattcta aaaatttatc aaaaatatag tcattatctt ttggtcctgg gcaagcttca
     2581 ggatggtatt gaacactgta tacatctagc ttctcatgtc gcattccttc aacagtatta
     2641 tcatttaaat ttatatgtgt cactttcatt tgttcaggaa cttctgaaac ataataacca
     2701 tgattttgag aagttataaa tacttttcct tcttctaaat ctttaactgg atgatttcca
     2761 cctctatgcc caaatttaag tttagcagtt tttcctccaa gagctaaagc taaaagttga
     2821 tgtcctaagc atattccaac tataggtttt ttaccaataa gagcttttat attttctata
     2881 acatcttcta agtcttctgg gtctccaggt ccatttgaca agaatattaa gtcagggttt
     2941 atactcaata catcttctgg agatgttgta gctggaaata ttgttatatc acatccacga
     3001 gctataaatg atcttaatat atttcttttt actccaaaat ccataacagc cacttttgga
     3061 ccatttcctt ttatatgctc tacttctttt cttgtaacag ttctaactgc ttctgtattt
     3121 gaaaacttat ccaatttatg tttaacatct tctaatttac tgtcttctaa tgtaattata
     3181 cctttcattg ttccattatt tctaagtatc ttagtcaaag ctcttgtatc tattccttca
     3241 agtccgataa ctttattttg ctttaaatat tggtctatat caatttcaca tctaaaatta
     3301 cttggagaat cacttttctc ccttactatg aatcccttta cttgaacttt ttcagactct
     3361 gctgatgata aatttatacc ataatttcct acaagaggat aagtcatagt aactatttga
     3421 ccatagtaag aagggtctgt taaaacttca ccatatccta tcatcgaagt attaaatact
     3481 acttctccta cactttcttc taaatatcca aatgcctttc ctataaaaac tgttccatct
     3541 tctaatatta accttgcttt catactacag tacctcctat aattctcttt agacaaacta
     3601 aagaacatta actaaatata agcttttaaa tatctatatc tatttttcac ttttacattc
     3661 tagttttata cttatttaca tttatttttt atctttttct attatactac aaatttctta
     3721 aaaaaaagaa gttatctcaa aagtaaaaac tcctagaaaa aataacctct tttttttatt
     3781 taataattaa attcttaaac tatacaatca acttaatttc tattcagcta tattgtaaac
     3841 ttttaactca tctttattta aatgtttctt cttaacttct actaaagctt ttagtgtatc
     3901 caaagatgtc attatttcag tagaaaattc aatagctgtt cttcttatct taaatccatc
     3961 tcttgtagag tcatttcctt tagttggtgt atttattact atatcaactt gtttatttct
     4021 tatagcatct aatatgtttg gtctagattc ttctactcta tttactatat cagcctctat
     4081 tccattttct cttaagctat t
//
LOCUS       DAYAOX010000272         4062 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.275, whole genome shotgun sequence.
ACCESSION   DAYAOX010000272 DAYAOX010000000
VERSION     DAYAOX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.275"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            130..1038
                     /locus_tag="ACGI57_003414"
     CDS             130..1038
                     /locus_tag="ACGI57_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HFG9718463.1"
                     /translation="MNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKCMNV
                     PIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSINAF
                     IKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINRLPV
                     LIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDIETL
                     NTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKYYLE
                     KTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            1522..2940
                     /locus_tag="ACGI57_003415"
     CDS             1522..2940
                     /locus_tag="ACGI57_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFG9718464.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            3031..3828
                     /locus_tag="ACGI57_003416"
     CDS             3031..3828
                     /locus_tag="ACGI57_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9718465.1"
                     /translation="MFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTEQLPKL
                     HQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRNYLMNL
                     KGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAVPDYCF
                     NIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYSTLGKTY
                     ADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
BASE COUNT         1565 a          397 c          706 g         1394 t
ORIGIN      
        1 aaatgttata tgtacggaag gtttacagga taaaatataa taaaaagatt aaggtgacat
       61 tataaaaaat gtatattgac attgacgcta cgtaattgta tatactttgt cttaaaggag
      121 atggtaaata tgaatattat agaaattcgc tcagataaaa tatacaagaa gataatggat
      181 gcaccaataa acaaaaaaga agatatatac agatatgaat taatgaagcc ttttgaattt
      241 aagtggaagt gtatgaatgt tccaatagtt gctagacaga aaggtggata tgatgtaatt
      301 atagcaagtg aaatgttagg ggttttatcg cctaaggata ttgatgaaaa gcaaaaaaag
      361 aatataaatg tgttatctgc tgataaaatt tggggcactt gcaaagaaac catagaaaac
      421 tctataaatg cttttataaa agaagggtat gatttaaaca ttaaggacta taaatattca
      481 atattattgg cgaatccaaa tagtccttat acaatattaa gtgatggata ctggggtgat
      541 ggtgggattc ctggatatat atttctatca ttggttccta atgaatatac tatcaataga
      601 ttaccagtat taatagcaca tgaatgtaat cacaatatta gatttcagtt tatagagtgg
      661 aataataata taacattaga agaaatgatg ataaatgaag gtcttgcaga aaattttgca
      721 acatggatgt ttggagagga aatgttagga ccttgggtca gtagaacaga tatcgaaaca
      781 ttaaatactt atataaagcc aataataaaa agtgctttaa aagaaactgg atttcaaaat
      841 ataacatctt atctttatgg tgatgatata gctaaaatgc aaggatattt tccagtaggg
      901 ttgccttatt gtgcaggata tgcttgtgga tattatatga ttaagtatta tttagaaaag
      961 acaaataaat caataatcga agcgacttta ttgccttata gtgagataat cgaagcagta
     1021 aaagagtttt gggaataaat tttataaatt gcataatttg aaataaatta tgcaattatt
     1081 ttatttttaa gaaaaaataa attgacaata atttataaga aatatacaat aatatatata
     1141 aagaaatatt tcaatattta aaagttttaa gatttcatat ctagagtagc taacattaca
     1201 tgttagtttc ttgttactta tctaattact ttatatttcg tagttatata taaaattact
     1261 ataaaaaatg aaatatatta aacaatcaac tgaaaaatga aatgttaata aaaaaaaatt
     1321 caaattataa atattaaatg aatataatta aaacaaatat ataaatttaa gtataacact
     1381 cacattttaa tgtattgtga aatttttgtt gaactttatg taccataact tataaaattt
     1441 atgtcatgtt atatttgaat aagtaataaa attgtagatg taaattaatc tacaaaattt
     1501 atatgaaatg gagttgatgc tatgacagaa ttaatgatta taagtgggtt agtactattg
     1561 atttgtataa cttctagcaa ggtattgtat aaatttggag taccaatatt attgattttt
     1621 atcttactag ggatgttatt tgggtctgat gggatggtag gaatctattt tgataactat
     1681 gaactgacaa aacaattatg ttctattgga ctaatattta taatgttttt tggagggttt
     1741 ggaacaaact ggaatatggc taaacctgtt gctatacctt ctatattaat gtcttcactg
     1801 ggtgtaataa ttacggctgg ggttacaggt atgttttgcc acatggtaat aggaactagt
     1861 ttacttgaag gtttattaat tggggcaatt gcagcttcaa cagatgctgc atctgtgttt
     1921 gctgtgttgc gttctcaaaa actaaactta aagggttcac ttgcatcatt attagaagtt
     1981 gaaagtggaa gtaatgaccc aatagcgtat atgctgacac taattctttt gacattgatg
     2041 aataattctg gggtagaact tattgcacca atggtaataa aacaaatagc ttttgggtta
     2101 gctataggat ttatactagc aaaattatct gtttatattt taagacattc aaacttcgag
     2161 atagaaggat tttatactat atttataaca gctattgcaa tactttctta tgcatttagc
     2221 gagtatcttg gaggaaatgg atatttaagt gtttacattt caggtattat tataggaaat
     2281 agtaggatac ctcttaaaaa aagtttggtt catttctttg atggaatttc atggattatg
     2341 caaataatgt tattctttat gttaggattg ttatctttcc cttctaagtt accaaatgta
     2401 tttggtatag caatggccat atctatattc atgatagtta ttgcaagacc attagcaaca
     2461 tttatagttc tagcaaaatt taaattctca aataaagaaa aattattcat atcgtgggta
     2521 ggtcttagag gtgcggcttc agtagttttt gcaatttatg ctgttactca aggagtagcc
     2581 atagagaatg acatattcca tataatattt tttatagcgt tgctttcagt aggaatacaa
     2641 ggttcattga taccaactgt agccagaaag ttggatttag ttgatgataa tacacctgta
     2701 ttaaaaacat ttaatgatta tgatggagaa attagctcta aacttatcga agtaaatgtt
     2761 gatgagggta ataaatgggc taataagagt ataatagatt ctaatatacc agacgatata
     2821 ttaatagtta tgataaaaag aaaggaacag gttttaatac ctaaaggttc aaccattata
     2881 aaaacagggg atattttggt attgagtgga gatagaattg aagaattaat acaattataa
     2941 tttattttgt attgacttta acctaagggg aaagtttaaa cttaaattaa tcaaatctaa
     3001 tgcgcaggtg atgagatgtt ttctatagga atgttttcta aaataaataa aataactaca
     3061 aagacattaa ggtattatga agatataggg ttgttaaaac cagaatatgt agatgaattt
     3121 acaaaatata ggtattatac aacagagcaa ttacctaaat tacatcagat aataacttta
     3181 aaacaaatgg gtttatcatt agctgagata aaaaaagctg tagaaaatcc tgaagaagtt
     3241 gaaaatattt taatgaataa agaaaaagaa atgttggatg taattaaaag tgaagaatat
     3301 aaaataatta aactacgtaa ttaccttatg aatttgaaag gagaagtcaa tatgaatgat
     3361 gtaattatta agtctttacc aaaagttaaa gttgcttcta tgagaaaagt gttaaagggg
     3421 tatgatgaac tttttaatct atttccaaat gtaatggctg aagaaatgat gaaaacaggg
     3481 tgtacatgtg cagttcctga ttattgtttt aatatatatc atgatggtga atatagagag
     3541 actgatatgg atgttgaaat gtgtcaagct ataaatgatt taaaggaaga tacggatata
     3601 ttaaaattta aagaattaga tgcagttgaa aatgcagttt gtatattaca caatggtcca
     3661 tattcaacat taggaaaaac ttacgcagat gcatttaaat ggatagagga taataaatat
     3721 aaagtggttg gaaatcctag agaatcttat atagatggta tatggaataa agagagtgaa
     3781 gaagactggt tgacagaagt tcagatacca gtgaaacgca tgttataata tgtctacaaa
     3841 gtataaataa gaataaaaaa ggtggtgtct caaagtaaac attttagttt atgagacact
     3901 ctttttcata tgaaatcaag tatattttta taattacgat tatgaaaaaa ggcttatcta
     3961 tatttttaga cagcctctta tttttttatt ataatagttt tctaaagatg ctctatttat
     4021 aaatttttat taaagacttt ataaataatt gtatttaata ta
//
LOCUS       DAYAOX010000273         4036 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.276, whole genome shotgun sequence.
ACCESSION   DAYAOX010000273 DAYAOX010000000
VERSION     DAYAOX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.276"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            50..958
                     /gene="garR"
                     /locus_tag="ACGI57_003417"
                     /pseudo
     CDS             50..958
                     /gene="garR"
                     /locus_tag="ACGI57_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
     gene            1094..2476
                     /locus_tag="ACGI57_003418"
     CDS             1094..2476
                     /locus_tag="ACGI57_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HFG9718466.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            2547..3983
                     /locus_tag="ACGI57_003419"
     CDS             2547..3983
                     /locus_tag="ACGI57_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFG9718467.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNGGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
BASE COUNT         1478 a          451 c          821 g         1286 t
ORIGIN      
        1 tggtatgtag tatactctaa aacataaaaa acgttttcaa aaatgtgaag atatggaggg
       61 ttataaaatg aaattgggat ttattggatt agggataatg ggaaagccaa tggcaaaaaa
      121 tttattaaaa gatggatgta atcttttggt gtatgacatt aataaatctg ctgtggatga
      181 acttatatca tgtggagcaa aatacgcaag tgttttagaa atgggtcaag aatgtgatat
      241 tgtatttaca atccttccaa atggaactat tgtgcaggat atattgtttg ggatggatgg
      301 attggcaaaa actttaaaag aaggaagtat agtagtcgat atgagttcag ttactcctac
      361 agaatcaata ttgtgtgcaa ataaattaaa agatatgggc ttagaattta tagactcacc
      421 agtaagtgga ggggagccaa aagcgattga tggtacactt gcttttatgg ctggtggtaa
      481 ggaagaaatt tataagaaag tagagccatt ttttaatata atgggaagct catctatttt
      541 aattggagat aatggtagtg gaagtgttac aaaacttaca aaccaagtaa tagttaattt
      601 gacgatagca gcagtttctg aggcatttgt acttgcagca aaagcaggtg cagacccaga
      661 aaaagtatat aaggcaataa gaggaggcct agctggtagt acaatattgg atgtgaagat
      721 accaatgatt atgaatcgtg attttaaacc aggtggaaaa atatctatta atttgaaaga
      781 tattaagaat gtaatgcaaa cagcacacaa tttagatgta ccactaccta tgacaagtca
      841 attattagaa attatgcaga cattaaaagt acatggacat ttggaagatg accatagtgg
      901 aatagcacag tattttgaaa agttggcagg tgttgaaata aaaaaacacg ataattaata
      961 tttaaatata tcaatataat taaatacata ttattaggat tagattatat aaatacagcg
     1021 aggttaagtt atcatagtaa aagaaaaata aaattgataa ataataaagt agaatgataa
     1081 aggggtaaag attatgatta caggatatgg acttttgata gcatttgtta tatctatagg
     1141 aattttactt gtatcgatta ttaaatttaa agtaaatccg tttttggcat tactgataac
     1201 atctataatt acaggcttta tggttagaat gcctataaat gaaatatcaa ccacaatatc
     1261 gactggattt ggaaatacat taggtagtat aggtattgta attggacttg gaataatatt
     1321 tggaaatatt ctttcagaat caagagcaac agaatctatt gcaaaggggt tgttagctaa
     1381 gactggagaa aaaaacagtg ctttagcagt aactacagca ggttttttaa tttcaattcc
     1441 agtttttatg gatgcagctt ttgttataat gatgcctata attaagtatg tttcgagagt
     1501 aactaagaaa tctctaatgg tttttgtatg tgccttagga gttgggacta tagtaggtca
     1561 tgcacttgta atacctacac caggaccttt ggcagttgca gcaaatgtaa atgcaaatgt
     1621 aggtagtttt atactttatt ctataattgt tgcttttcca gcagctctta taggtggatg
     1681 gatttatgga aaacgttttg aaaaatatcc agcttatgct attgatgaga atgatagaga
     1741 aaaaaattta gagcagggaa aagatagcat aaaaattgaa gatgatgatt cttctaaagt
     1801 accaggtttt ggaatttcta tgttttcttt attgttccca atacttctta tacttatatc
     1861 aaatgtattt agtatgttct tagaaaaagg ttctacaatg agtggagtac ttgcatttat
     1921 aggagataaa aacatagcaa tacttcttgg aatattggtg gcaataggat ttttaaagaa
     1981 atacataaat aaacctatgg gagatgtagt tattgaagct gctgattcag cagggctaat
     2041 attattgata acagggtcag gtggtgcgtt tggcagtgtt ataaatgcaa gtggtatagg
     2101 taacttctta gttgatacta tgtctggtct tagtatatca gttgtagtac ttggattctt
     2161 attaagtgcc ttgcttagaa tttctcaagg ttctgctact gtagccttag taacaacatc
     2221 atcaatttta ggacctacaa ttttggcaac tggaatgtca ccagttttag tagggcttgc
     2281 tatatgtgct ggaggagttg gtttctccct tccaaatgat tctggattct gggtactttc
     2341 aagatttagt ggattatcag ttaaagatac tttaaactct tggacaattg gaggaaccat
     2401 tgctggagtt gtagcattta taatggtttt attgcttagt gttataaatg gaattatacc
     2461 acttccaggt ctataaataa caaactattg tttatatcat ataaattata tttagactat
     2521 acaaattaca taaaggggcg ttttaaatga gagaagtaaa accaattagc atagatatac
     2581 ttaatacttt taaacaagta gatgaggata gattaaataa acttcttgca gatgagctta
     2641 agcatttaga tagaaaaatt gttgtattag atgatgaccc aacaggtgta caaacagttc
     2701 atgatatttc tgtttatact gattgggata aggatagtat ggaacaaggc tttaatgaga
     2761 aaaatagtat gttttttata cttaccaatt caaggggatt tactgttgct caaactacaa
     2821 aagcacataa agaaatttca aagaatattg tggatgtatc aaagaaagtt aataaggatt
     2881 ttataatcat aagtcgtagt gattctacta tgagaggaca ttatcctctt gaaactaatt
     2941 tattaaaaag cgaagtagag agattatcgg ggaagttatt tcagggtgaa ataataatgc
     3001 cattttttaa agaaggtggt aggtttacta tagataatgt acattatgtt aaagaaggag
     3061 aaatacttgt accagcagga atgactgaat ttgcaaaaga taaatctttt ggatataaat
     3121 catctgatat aggtgagtgg tgtgaagaaa agacaaatgg aaagtacaaa tctagtgata
     3181 tgatatatat ttcgtcaaaa gaacttagag agctaaatat tgaagctata acagaaaaac
     3241 ttaaaaaagc tgaaggattc aataaaatta ttgtaaatgc aaccgattat gtagatgtaa
     3301 aggtatttac aattgcgttt atacgagcag taaatggtgg taaggaattt atgtttagaa
     3361 gcgctgctgc cataacaaaa gtacttggcg gagtgagtga caaggaacta cttactaagg
     3421 atgaattagt atcaaaaggt aataccaatg gaggaattat acttgttggt tcacatgtga
     3481 ataagactac ccaacagtta gaagaactta aaaattgtaa atatccgata gaatttatag
     3541 aatttaatca acatttagtt cttcaagaaa atggtctaaa aaatgaggta aaaagagtta
     3601 taaaagttgt agaggaaaaa ataagcagtg gtaaaactgt tgctgtttat actagacgag
     3661 agcgttttga cttggatacg aatgataaag ataaacagct tatgatttct gtagaaatat
     3721 cagatgcagt taccagtata attggtatgc ttaatgtaag accaaatttt attatagcta
     3781 agggtggaat tacttctagt gatgttggta ctaaagcact aagagtaaaa aaagctacag
     3841 taatgggaca gataaaacct ggtataccag tatggatgac aggcaaagaa agcaaatttc
     3901 ctaatatgcc atatataatt ttccctggaa atgtgggaga gattagcaca cttagagaat
     3961 cagttgagat attgatgggg tagtatatta gtgtattgaa gtttaaacgc ttaaatttac
     4021 tgagttaaaa ttaaaa
//
LOCUS       DAYAOX010000274         3948 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.277, whole genome shotgun sequence.
ACCESSION   DAYAOX010000274 DAYAOX010000000
VERSION     DAYAOX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.277"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            149..1729
                     /locus_tag="ACGI57_003420"
     CDS             149..1729
                     /locus_tag="ACGI57_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFG9718468.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1903..2349
                     /locus_tag="ACGI57_003421"
     CDS             1903..2349
                     /locus_tag="ACGI57_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFG9718469.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2370..3413
                     /locus_tag="ACGI57_003422"
     CDS             2370..3413
                     /locus_tag="ACGI57_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFG9718470.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1601 a          357 c          716 g         1274 t
ORIGIN      
        1 ttaattcaaa attttttgtt attttaaatt atttcaaaac tcttagtttt agtgatttta
       61 tttatgatat ttatgggatt aaaaagattt caatagatat ttttggtaag tatttaagaa
      121 gtctttaact ttataggagt gataatttat gctaataggt acatctaaat taagcagaaa
      181 tgttgatact gcatgtgtag aagaagtagg gattccaatg attgtattga tggaaaatgc
      241 ggttatatct gcaatgaaaa atatggatat agacatgtat aatagttata cagttgtatg
      301 tggtgttgga aataatggag gagatggtct tggaatagca agacatctaa atacatatgg
      361 aaaagaagta aacgtatttt tagtaggtaa tcttgaaaaa ctaagtgaat gctctaaaat
      421 aaattacaat gtacttttaa atatgggaat taatattata aatattaata aaaatgattt
      481 aagtgaaaaa aattatggag aggaaaatga agtagataaa attattaaag ttgagaaaca
      541 aaatattata aaaaataaaa atttaacaat ttttaaagat agagtatcta aaagtgatgt
      601 tgtagtagat gctatatttg ggactggatt aaaacgtgat ataactggta tttttaaaga
      661 agttattgac atagtaaacg ataatagcaa aaatacgtat tctatagata ttccttcagg
      721 tatagatagc gataatggaa atgtattagg aacttgtatt agagcaaaca aaacgattag
      781 ctttgagttt tataaaagag gttttctaaa ctatgacact agcgcactta taggtgatgt
      841 agttgtagaa aagataggtg ttccagattt tattacaaga aaataccatg atgaagagtt
      901 cataactgat aagacttttg taaaaaataa tattagaaga agagataagc atggttttaa
      961 aagtgatttt ggaaaagtat ctatagttgc aggttcaaaa ggattttatg gtgcaagttt
     1021 catagcaact gaatctgctg ttaagagtgg gagtggactt gtaacattaa ttagtagtga
     1081 agatgtgcaa gaaaaggtat ctattagact tacagaagca atgactttaa attttgaaga
     1141 agaaagatta aataaattac ttaattctag tgatgcaatt ggttttggtc caggtatggg
     1201 agataatagt caaacatttg ataaattact taagatagta gaaaattcta attgcccaat
     1261 agtattagat gcagatggat tgaacgttat gaaggacaga tgttataagt ttttagaatg
     1321 gaaaaataga tttgtaatta cacctcattt gggagaaatg gccaggttga caggagattc
     1381 aattgggtat ataagagaac atagagtaga tgttgcaaaa gaatttgctc aaaaatataa
     1441 tctggtggtg cttttaaaag gttatcaaac tgtcataaca gatggtaaaa aaacatatat
     1501 aaatcctaca ggaaatagct gtatggcaac tggtggaatg ggtgattgtt tgcttggaat
     1561 gattacttca tttataggtc aaggtatgga tatattggaa gctacagtta gtggagcata
     1621 tatacatggc tatataggag ataaattgag caaagcaatg tatacggtaa atgctacgga
     1681 tttaataagt aacatttcat taacaatgaa agagttgttg gatatataat tggtgatatt
     1741 tgatactact ttgtaacaat tttaacaaag tatgttgata taatactatt aattaattaa
     1801 tttattttat tgttttgtaa tttaaattta tattcaaaaa cattgtataa acatataatt
     1861 tatatagggt tttataattc atatgaaaag agggtagata gtatgaagct aatatggttg
     1921 atagtagcag tcttatttgg aatagcagaa atgttaacac caagcttaac attgatatgg
     1981 tttagtgtag gagctgtaat acttatattt ttaagtagct ttatagagag tattttctta
     2041 caaattttaa tatttgcagt aatttctata gcaatgttag tcgtagctac taaaaaaata
     2101 gtaaaaaaag ataaaggcta taaatctaac actaatttac aagcaatgat gtctaaaaaa
     2161 ggtattgtta cagaagagat ttctccaaat aacacaggtc tagttgtagt tgaacatgaa
     2221 acatggacgg ctatttctat agatggtgaa aagatagaaa aaggttctac agtagaagta
     2281 ttaaaaattg aaggggtaaa attagttgtt aaaaaagtag acgccacagt taatgtgaca
     2341 aaccaataga ataaaggagg atttatagta tgggtactaa aattgtttta agtattgtat
     2401 taatagtagt agtcgtagca ataagtttaa cttgtataag agttataaaa caatctaaag
     2461 taggtataat aatgagactt ggtaagtttc aaaaagtggc tgaaacaggt gtacatttct
     2521 taataccatt tttagataaa atggcatatg taattgacct tagagaaatt gtaatagatt
     2581 ttccacctca accagttata actaaagata atgtaactat gcagattgat acagttgtat
     2641 attataaagt tacagaccca gttagatatg tgtttgagat agctaatcca atagctgcta
     2701 ttgaaaattt aacagctaca acactaagaa atataattgg tgaacttgat ttagatgaaa
     2761 cattaacatc aagagatata ataaatgtaa aaatgagaac tattcttgat gaagcaacag
     2821 ataaatgggg tataaaagta aatagagtag agttaaaaaa tataatgcct cctcaggata
     2881 ttcaagttgc aatggaaaaa caaatgagag cagaaagaga aagaagagag gcaatacttc
     2941 aagcagaagg taataagtca gctgcaatac ttcaagctga aggtgaaaaa caatctgcta
     3001 tattaacagc agaagccaaa aaagaagcta tggtacgtgt agcagaaggt gaaaaagaat
     3061 ctgctatatt agtggcagaa ggtgaagctg aagctataag acagacagct attgctaagg
     3121 cacaaggtga agctgaaatg ataaaaagaa ctcaaatggc aacagcagaa ggtttaaaat
     3181 tagttttttc agcaatgaaa gaggctgata tagacaataa tattctagca ttaaaatcta
     3241 tggaagctct tgaaaaaatg gctgaaggta agtcaacaaa actggtttta ccttcagaag
     3301 cagttaattt cctaggaaca ttcaaaggaa taaaggaagt tatgagtgat gataacaaag
     3361 aagtacttga tataaaagaa gttttaaatg ataatgaatc attaaaaaaa taaaagagtt
     3421 ttgttatcat aggaaggata tggaaattaa aggtcgaatt aattaaatat agcacaaaaa
     3481 tgcacgttga aaagaagagg acttataaat ttttaagtct tcttctttgt ttttattaga
     3541 aagatgaaat tataaaaata aattaacaga atttttatac aatttatcct ttactaatgt
     3601 aaaaagtgtt atataatatt atataaaaca tatagcaaat aagcatggtt ttagttattc
     3661 tatttatatt attaatatat atttataaaa atataaacat taatactaag aaaactaata
     3721 aataatttaa aagtttatat ccttttgttt aaattataga caaagaagta tcaattaaaa
     3781 aataaagttg attaataatt acttgatttt ccttggataa ttatttaaca aatattagat
     3841 tatattttgc tattacgaat ttaacaaatt aaaaaataag catgttcata tgacacaaag
     3901 gatttataaa aaatgttttt atatttataa aataaaattt gtattatt
//
LOCUS       DAYAOX010000275         3912 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.278, whole genome shotgun sequence.
ACCESSION   DAYAOX010000275 DAYAOX010000000
VERSION     DAYAOX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.278"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..755)
                     /locus_tag="ACGI57_003423"
     CDS             complement(<1..755)
                     /locus_tag="ACGI57_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718471.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFT"
     gene            complement(761..1648)
                     /locus_tag="ACGI57_003424"
     CDS             complement(761..1648)
                     /locus_tag="ACGI57_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718472.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(1662..2684)
                     /locus_tag="ACGI57_003425"
     CDS             complement(1662..2684)
                     /locus_tag="ACGI57_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718473.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            3259..3435
                     /locus_tag="ACGI57_003426"
     CDS             3259..3435
                     /locus_tag="ACGI57_003426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718474.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         1400 a          601 c          373 g         1538 t
ORIGIN      
        1 gtaaaatttt catatgttat tttagttaac actatcatta atataattga gattactata
       61 gaaaaaacta tcaaaatact tggtacatta tacttaacca tttgttggct catcataatc
      121 caatttggag tcactgacat aaattttata gctttatctg gtataaaacc tacactaaag
      181 tatggaattg ccattatcaa tccacctagt ataaatggaa ttacagccga tttaaatata
      241 gatgaaagtg ttaagcctat ttgtgcaaat ataaaagaag caaaaatagt agtcaatata
      301 acaattatat acatttgtat tactgtaaga tttaaattac tatttccaaa gtaccataag
      361 ttatgaatcg attcatttaa atttttaaaa tcaaaaaaac taaaacctct aattcctact
      421 aacaatataa aagaaatatt aataatagat aaaattaatc cattagctac tagttttgat
      481 aatactactg atttttttcc ctttttagtt gtatttatta aacctgtcat attagaatca
      541 tattctttaa tgtaatttcc agaagttatt ataaacatta tcaaacctat tagaggtatt
      601 gagccttcta tcttgtaaaa tatatctgca taagtatcta aaacatcaaa ctttctaatc
      661 agaaaaaatc caaaaaatat taaaaacagc caaattatcc aaacactctt tgattttaat
      721 atttttatca tttcagcttt aactaaatac atcatttcta ctacacctcc cttgcatctt
      781 ctaaatcaaa ataaaacatg tatacatctt cgaattttgc ttgaactggt tgtatattat
      841 cctcgcttgg aatatcctct gaaacaattc taagtactac tgaatcaata tcactttgaa
      901 tacttacaac cttatattta gattgtatat catcaacttc acattcattt ttaacttgaa
      961 cattatatac tttattttcc atgtgactta atatgtcttt gtgtgttcct ttcattagga
     1021 gtttgccatc tttaatcatt attgtttcct ttgcaactga ttctatatcg gaaattatat
     1081 gagtagaaag tattattatc ttatctctag acatttttgc tattaaattc ctaaatctag
     1141 ctctttcttg tgggtcaagt cctgctgttg gctcatcaag gactattatc ttagggtcgt
     1201 ttagtaaaac ttgtgctatc ccaactctcc tcttcatacc tcctgaaaat tttcctatag
     1261 acttatttct cacatcataa agacctacta aatctaataa ttctcctatc ttactattta
     1321 aatattttcc ccttaaacct tttaaagctc caatatactg taaaaattct tttgctgtaa
     1381 aatttttata aactccaaat tcttgtggca aatatcctaa aatatttcta taatcagcat
     1441 ccattttaaa tatatcctca ccattataaa gtatctgacc ttcatcaggt tttatcaacg
     1501 ttgttatttg tttcattaat gtagattttc ctgctccatt tggtcctaga agaccataaa
     1561 ctccattttc cagagttact gtaatcttgt cgtttgctac ttttttacca tatatctttg
     1621 aaagattttc tatttctaat ttattcatct taactcccct tttatctact tatataatct
     1681 gattttatta ctaaaaaaca taatgacata gcacctatca atattgccat caaaagtcct
     1741 attgctatat tgacaatgtc tatattcact ttaaatatca taatatcacc tagataattt
     1801 cctattattt tattaaaacc aaatatcaaa tcataataat ttacatttga tacaactgaa
     1861 agtaattttt taggtaatat atggattacc gacattatct tagttataga tataataaat
     1921 ataaaaaaac ttattgaaga tattgaagac ttacttataa aagacccaaa tatggctatc
     1981 atggcccatc cttcaaacat caaaagctct atgcctatat ttaatattac atactttgtt
     2041 atacttaaat tagttttcaa tgcaactaca ttgtctacta ttctaaaaga ttctaagtct
     2101 ccattaacag gtactccttg ttgcatacaa gttataataa atattcccat taaataaaca
     2161 gaataaatta ctaatggaac taataaagca acaaatattt tagactttaa tgcttttttc
     2221 ttctcttttg atgatagtat gatatctttt accgaagtaa cgacttcatc tacataaata
     2281 ttggatataa ttatagatat aatgattaac attcctacat ttataagtga atttgtgact
     2341 ctatagttaa atactttcca aaaactaata tccttatatt cattcacact tagattatca
     2401 agttttttct ttgacattaa ttcaattttt ttagaaaatt cacctgtatc tgtttgattt
     2461 gaaaactgct ttaaaacatt aatcttattt tgaaatttct cattacctac gtcgatacca
     2521 cttctcgtat cttctatacg tcctttttct ttatctatat aataactttc tgtttctaaa
     2581 acaggcgtaa taaagatact aatcattagt attaacacta atatccctag agatataaac
     2641 atactcttag atttagccaa tttttttatt tcccaattta ccaatatatt tctcctccta
     2701 aatcatttta attacacata tatattaaaa cttaaatctt atataaatat aatactaacc
     2761 ttaaataaag cttaaattat tttataattt ataggtgtgt ttattattgg agttacattt
     2821 attatttttc tgggaaatta tacattttca attattttta ttatttttga tatttttatt
     2881 gattggaaat tttattgttg ttcaaattgt agttattctt taaatttaaa gatggagttt
     2941 tttaagaata gaacctactg tccttattat ggaaagatta ttaaatagtt gcatataact
     3001 ttatttatct tgaatcttat ttttaaaaac aattataatt ttagaatatt ttctattctc
     3061 aaacaagcat gttgaataat ggagcatctt ctagtattat atacttgtag aataaaacta
     3121 aattggtaaa actgggaata tttaacctta aggagttaag ttatagaaac tagtactaaa
     3181 aaattttata aattatgtta attgactaat agaatgcgac tttttatttt tactaaattt
     3241 tttaggggga tggataatat gtttattgga gaaatagaca aatgtacaca tatactaact
     3301 gcttacatca gtagttcata tgattattgt aattttttag atacacagtt agatgatttt
     3361 atatcagaat atggagaaac ggtagttgaa acctgcttac atcaagtctt attattagta
     3421 agtagataca attaaaacta aatttttcta gaaaaacctt tttagattta tctactctaa
     3481 taaatatata attttttgta ctttataagt aactataact tctgctaaag taaagatgta
     3541 ttgggagtac atctttactt tcccattata gaagttaaaa gcatacacca taaaattaat
     3601 catccaatac aaattatata tttcctatgt ctaacttaat ttacaagaat cattctatat
     3661 gcatattctg caatccacta aacatggctc taaaccttta ctattttata cattctctat
     3721 aaatcataca aatcatatat aaaatccccc taatatgttt aaaccaccta taaaataaca
     3781 ccaatattta gaatattcaa tatcaactgc ataactggtc aataaaaacg tataactaga
     3841 caaatttcaa tatttatata aataatacaa gaaaaacaag cattttattt atataagttt
     3901 tttatttttt gt
//
LOCUS       DAYAOX010000276         3908 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.279, whole genome shotgun sequence.
ACCESSION   DAYAOX010000276 DAYAOX010000000
VERSION     DAYAOX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.279"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            62..283
                     /locus_tag="ACGI57_003427"
     CDS             62..283
                     /locus_tag="ACGI57_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718475.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            789..1658
                     /locus_tag="ACGI57_003428"
     CDS             789..1658
                     /locus_tag="ACGI57_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9718476.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            1884..2750
                     /locus_tag="ACGI57_003429"
     CDS             1884..2750
                     /locus_tag="ACGI57_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9718477.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            2942..3139
                     /locus_tag="ACGI57_003430"
     CDS             2942..3139
                     /locus_tag="ACGI57_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9718478.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            3142..3585
                     /locus_tag="ACGI57_003431"
     CDS             3142..3585
                     /locus_tag="ACGI57_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718479.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(3772..3859)
                     /locus_tag="ACGI57_003432"
     tRNA            complement(3772..3859)
                     /locus_tag="ACGI57_003432"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3823..3825),aa:Leu,seq:caa)
BASE COUNT         1513 a          388 c          677 g         1330 t
ORIGIN      
        1 taatatagaa tacattaatt aagtttttga catgtaacta tactaataag gaggagtatt
       61 tatgaatgta aataatcaca agtgcccaga gtgtggtgaa catagtaaaa agtttgaaat
      121 agtagaggaa aatgaggatt gtgtaatatt tgaatgttac acttgtggaa ctatatctat
      181 attaaagtat tgtgagaact gtaaagaatt tgtatttgta aatattccaa ataatgtgga
      241 gacatatgag tattgttgtg gaaaatgtaa taataaaatt taatataata taaacatatg
      301 taatttcata tatcatttat accaatctca tataaaattc atatataaaa tgtattatat
      361 aaatatagaa agaactagct taaaacataa tataattacc ctaaatttat aatgacaact
      421 ctccctcaca tcctcaaatt atagttgtca ttatagaata acttggctgt ttcaaaaatg
      481 tgaaatcatt ttagaaacag cttatttttt tataaaaata aagaaggcaa cgggaagcct
      541 tctttcgtgt aaaaaaagtt taattttttg ttaagatttg tttgttattt tatattacct
      601 ttataatatg tttttatttg gtggttggca atggattttg cacaactggt cattattaat
      661 gtaaaagtgg aatattatta ataaataata attacaaaat tgtaatcttt atttattaaa
      721 taaatgagta aaataatata ttaatgggta tataattaag acaatactag tttggggagg
      781 ataaaattat ggattataaa acaatcatat taaatagaaa gtctgttaga gaatataaga
      841 acacagatat taaagaagaa tatcttagtg aaattaagaa atactcagaa tcttgtaaga
      901 aactagttcc agaaatagat gttgatgtaa gaattatgaa tagaagtgaa gtttttaata
      961 atttagataa agtagcaggg tacaaaggaa atatgataga ttctccaagt tatattataa
     1021 tattatctga cataaaagat aattacattg aaaattcagg gtacatagga gaaagtataa
     1081 tctttaaagt aacagaactt ggaattggtt cttgttgggt tacttttgaa gatggaaaaa
     1141 aggtaaaaga aaaacttgga attaattcag ataaagaggt aacaggaatt atagctattg
     1201 gatatggaga aaatgtaagt aatgctaaag tattaaatgc tacaaaaact gggcaaaatt
     1261 attcaaaatc tgatatgcaa gttgtaacag ataaggggtc tactagacta ggtgtagaag
     1321 atgttgttta tatgaacgaa tggggaaatg ttgcaagtat agaaatctta gaagaaagag
     1381 cacttcttga tgcatttcac tatgcaagat tagctccatc tacattaaat agacaaccat
     1441 ggagatttat agttgatgga ggcactgtag ttttagctgt tagaaaagat ggtcatacta
     1501 atttatatga agaaaaaata gacattggta tagttatgct ttattttgca acaataataa
     1561 gtgcaactat gtttgattta aaatggaatt taggtactcc agacaaagat tataaagtgc
     1621 cagaagacta taagatagta ggatattgta atatataaga tagtgtaata cttaatttaa
     1681 ctacacttaa attaattttt ataaaaagtt caataacatt ttataatata atgttattga
     1741 acttttttat tgtataatac aatttttata tttattgtat tgattgaaaa atgcttataa
     1801 atttaaatgc ttacaatttt gtgcatgaat agagaggtat attattttat taaactgtat
     1861 aatttgcttg gaggtaatat caaatggaat ggataaataa attaaatcaa gctattactt
     1921 atattgaaga aaatctcgaa agtggagtgg attatgcaga agcagcaaaa attgcatgtt
     1981 gttcaacctt tcattttcaa agaatgtttt catatattgc agaagtacca ttatctgaat
     2041 atattagacg aagacgtatg actaaggctg catttgaact tcagaacagt aacatcaaaa
     2101 ttttagaact atctgagaag tatggttacg attcaccaac atcttttaat agagcttttc
     2161 aaaatataca taacatttct ccatctgcag ctagagcaaa gggtgttgtt ttaaaagcat
     2221 atcctaagat gacattaagc atttatgtaa aaggaaatat agaaatgcaa tatcgtattg
     2281 tagaaaagaa agggtttcgt attgttggta taaaagaaag tatgaacatg attgtagaag
     2341 agtgctttga gaaagttcct aagttgtggg ctaaatgcat aaaaaatgga acaattgata
     2401 aactttcaga gttaataaat aatgaacctt gtggattgct tggagttagt gtgtgtacaa
     2461 atagtaaggg tttagattat tatattgctg ctccaacaga taagcctgta ttagaagata
     2521 ctcatgaata cttaatacca tcaggcacat gggcagtatt tgaatgtgtt tctccaatgc
     2581 caaatgctct ggcaatacag gaattgcaaa aaagaattat tactgagtgg ttaccaagtt
     2641 ctgggtatgt gtatgctaat ttaccagata ttgaacttta tcccaatggc gatatcaatg
     2701 ctcctgatta tactacagaa gtttggattc caattcagaa accaagttaa aattaataaa
     2761 taatatggaa ttaataaata acattaaaat taataaacaa catgaaattc taaaattaca
     2821 ggctgaaaat agtctgttat ttttttgtaa atatggaaag taagctttac ataaatggaa
     2881 tatagttgta taattatgga aagtagactt tccgtagttg agatattaaa gggggataga
     2941 tgtgaaaaat agattagaag aaataagaaa agaaagaggc ataaaacaag aagaacttgc
     3001 ttctattttg caagtatcaa gacaaactat aggttcatta gaaaatggta ggtataatcc
     3061 ttctatcatc ttagccttta agatagctag atactttgat atgagtattg aagaaatatt
     3121 tatttatgag gaggagtaaa aatgaaaaag aatatgttat atgttggaat tatatatttt
     3181 atttgtggaa ttttacttgt tttattggct acatttactg agttttcatt tgaggctttt
     3241 atttggggat tatctggagc tgctttaggt ccaggtgtat gtatgattct tcaatatatg
     3301 tattggagta aacctgaaag agcagtagat tatgaagaaa agataaaaaa tcaaaggata
     3361 gagatgaatg atgaaagacg aattatgtta cgtgataaag ctggacgtat cactaatcaa
     3421 atcatgtcct atgttcttgt aattcttata tttattgttt ctatattgag tgtattttct
     3481 gtaatggtaa taagcaagtg ggttttagtt gttcttggat tgcttatttt atttcaattt
     3541 atttgtagtg ttgttgtata caataaatta gataaaaagt tgtaataagt tatgatataa
     3601 aatagaaaat tatataaaaa taaattagtt tttaaattaa tgctagaaga taaatttata
     3661 taattatata aattttaaaa agacacagta atttcaaaaa ataacttaaa atttaaatca
     3721 aaaataaaaa aactcctaat ctattgaata aatagcttta ggagttttta atggtgcccg
     3781 aggccggact cgaaccggca cggagtatta gtccggtgga ttttgagtcc actgcgtcta
     3841 ccaattccac cactcgggca tgtcatgtat attattatag caaaaaaata tattgtttgc
     3901 aacatatt
//
LOCUS       DAYAOX010000277         3830 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.280, whole genome shotgun sequence.
ACCESSION   DAYAOX010000277 DAYAOX010000000
VERSION     DAYAOX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.280"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            78..1190
                     /locus_tag="ACGI57_003433"
     CDS             78..1190
                     /locus_tag="ACGI57_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9718480.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1398..1874)
                     /locus_tag="ACGI57_003434"
     CDS             complement(1398..1874)
                     /locus_tag="ACGI57_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718481.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2073..3698)
                     /locus_tag="ACGI57_003435"
     CDS             complement(2073..3698)
                     /locus_tag="ACGI57_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9718482.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1335 a          613 c          422 g         1460 t
ORIGIN      
        1 gcaactctat ttcaaaatta ttttgtgata tatatatgtt ataataaatg tataaaattt
       61 tgattggtgg gttatatatg aatgcattta ttagaaaaag aaataaaaac tatgttgttt
      121 acctggaatt tgaagatgaa aagacaggta aaagaaaaca aaaaaatatg ggtacttttg
      181 ataaaaaaag ggaagcaagt aaaagactaa atgaggtaaa agaaagctta tataatgatg
      241 gttttttagt accaagcgaa attacagtgt cagaatttct attagatttt ctaaataaat
      301 atagtgaaaa tatatcttta gctacatata ataattatgt tagtatttgt aagaattaca
      361 ttaatccatc tattggaaaa tataaaatac aagacttaca tccggttcac atacaaaact
      421 acatagataa actatcttat aaactaaatc ctcaatcaat taagattcat ataaatatat
      481 tgaaacttgc aataaaaaga gcttatagac tcaaattaat aaaagaaaat gttatggata
      541 gtatagaggc tccaagatat aaaaaattta aaaatgagat ttatgataga gaacaaatga
      601 taaaattatt agatttagca aaaaatacag aaatggaatt acctattaat ttggctattg
      661 gattaggtct tagaatttca gaagttttag gtctaacttg ggataatatt gattttgaag
      721 aaaatacaat aacagtaaat aagataactt cccgaattaa tggttctgtt attctcaaag
      781 agccaaagac agaaagctct gttagaaaaa tatcttctcc caaagaatta atgtctttat
      841 taaaagaata taaaataaaa cagaataaaa atttattaaa atcttccatt agaaataata
      901 ataatttgtt atttttcaat aagaaatgtg aacctattgc tgaagatgtt atgagtaaaa
      961 aatttaaaag atttttagaa aagaatgaat tacctcatat tagattccat gatttaagac
     1021 actcacatgt tactttactt ataaattcta aagtacctat aaaggttata tccgagagag
     1081 taggacactc aaatataagt actactctaa gcgtttattc tcatgtactt aaagaaatgg
     1141 ataaagaggc ttctgacaaa atttctgaaa gtttatttaa tgctaattaa tattaattta
     1201 tcttagcttt aaattagctt agcttattga gatagattca ttaatattaa atgtatctat
     1261 ctcaaattaa taactactta aataatctgt tttattttaa tttatcgcct cagttattat
     1321 acttttttac gaaaaattat actttacaat aaaaaagata ttcccttata gaaaatatct
     1381 ttttaaatac tatattatta tatacttgct aacattggaa tatctaacat cttcttcatt
     1441 acatcatcta ctttttttgc tttatattca ttgtatccaa ttaaatatcc tttttcatat
     1501 gaacttttta aaatctcttt ttcatataaa ttatcagatt tggcttctgt gacacttaaa
     1561 ttataaagcc ctttacaccc atcttcataa cctttattat accctttttc tgcaaaatag
     1621 tttttatgat ttttttcgtt atgtactgaa gatattgtta ctccagaatt ttcttttgta
     1681 ctagcataag gacaaaaacc tttattgtgt tgatgtgctg aatgtcctcc acaataataa
     1741 cgtgttaact ctgatttatt tttattatga tgacctcctg atgaattatg ccctcctgag
     1801 tgagcatatg aaaaattccc tacactaatc atagctaaac taattactac agctactaac
     1861 cttttcttaa ccataatcta ttccccccaa aatgttatat atttttatta tatcatctac
     1921 tatattatat ttaaattctt aatatacaaa atattactct attctgctaa taaacatgtt
     1981 taaacaatat aaaaaacctc ctaatatttg attaaaaatt tacacactca tgcatagcat
     2041 gtctcaaata ttaaaaggtt ttatactaat aattatttat cttttccata cattttagct
     2101 aattggtctt gtatctcttt actttctaaa tactcatcaa agtccatttt tctatccatt
     2161 attccatttg gagttatctc tataattcta tttgctatag tagatataaa ttcatggtca
     2221 tgagaagtaa ataaaagcac tcctgggaac ttttcaagtc ctttatttac agaagttata
     2281 ctttctaggt ctaagtggtt agtagggtca tctaatacaa gtacatttgc atttgaaagc
     2341 attagcttac taagcataca tctaaccttt tctcctccag atataacttg agcttctttt
     2401 aatgcttctt cacctgagaa taacattcta cctaaaaatc ctcttataaa actttcactt
     2461 ttttcttcag agaactgtct aagccagtca accaaagaat attctactcc gtcaaaaaac
     2521 ttattatgat tttttggaag ataatcttga gaagtagtta ctccccattt atactctcca
     2581 ctgtctggtt caagttctcc cataactatg tttaaaagtg cagtagtagc tatttcatct
     2641 cccataaata ctactttatc atctctatcc aatctaaatg acacattatc aagaacctta
     2701 accccatcta tagttttagt taaattgtgt acttctaaaa cttcattccc aatttctctt
     2761 gcaggtgtaa atcctacata tggatatctt cttcttgatg gttgtatatc ttcaacttct
     2821 aatttctcta actgtttctt tcttgatgtt gcttgtttag cctttgaagc attagaacta
     2881 aatctagcta taaattcctt taattgagct atcttttctt cagtcttttt attttggtcc
     2941 ttagccaatt gtaaagccaa ttgacttgac tcataccaga aatcatagtt tccaacgtac
     3001 atttgtattt taccaaaatc tacgtcaact atattagtac atatttgatt taggaaatgt
     3061 ctatcatgtg atactattat aactatagat tcttctaaat ccattataaa attatttaac
     3121 cagtttattg atttaaaatc taagtggttt gtaggttcgt ccattaatag tatttcaggc
     3181 ttaccaaaaa gtgattgtgc taacaatacc ttaacctttt ccccaccaac tagttccttc
     3241 atttgtttgt aatgcatatc tttcgttatg ccaagaccca taagtatttt ctcagcatta
     3301 gtttctgcat cccatccgtc aagctctgca aactcacctt caagttcggc agcttttatc
     3361 ccatcttctt cattaaagtc ttccttcata tacaatgcat ctttttcttt cattatgttc
     3421 caaagtctct catgacctct tataacaaca tttaaaacag tttcttcttc atattcaaag
     3481 tggtcctgtt ttaaaactga cattctttct ttatcagtta tagatatact acctgtatta
     3541 ggctctattt ctcctgataa tatttttaaa aaagtagatt ttcccgcacc attagctcct
     3601 attattccat agcaattacc tttagtgaat tttaagttaa catctttaaa tagttcctta
     3661 tcaccaaatc taagtccaac acctgtaact tgtaacatta atatattttc ctttcttttt
     3721 gctcataatt tattttatca taaaactttg ataacatttt tattatatat tatcacgtaa
     3781 atagtatatt ttttatacta aatttgtgaa atatttatta aaataatact
//
LOCUS       DAYAOX010000278         3805 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.281, whole genome shotgun sequence.
ACCESSION   DAYAOX010000278 DAYAOX010000000
VERSION     DAYAOX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.281"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            227..1855
                     /gene="aspD"
                     /locus_tag="ACGI57_003436"
     CDS             227..1855
                     /gene="aspD"
                     /locus_tag="ACGI57_003436"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047688 - aspartate 4-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HFG9718483.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1976..2971
                     /locus_tag="ACGI57_003437"
     CDS             1976..2971
                     /locus_tag="ACGI57_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFG9718484.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3149..3457)
                     /locus_tag="ACGI57_003438"
     CDS             complement(3149..3457)
                     /locus_tag="ACGI57_003438"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9718485.1"
                     /translation="MSKDVADEIEKTYAWKHTTTITLLARLTKKGFFISQRIGKHVHY
                     RVLIKEKEYLKIEGKRIFGGLHNNPLSELISKLHDGEEITEEKIIEIGNWIKSWKDED
                     "
     regulatory      3759..>3805
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         1355 a          511 c          578 g         1361 t
ORIGIN      
        1 aactaaggtg ataattaaat tgaatattta ttttatataa caatcatttt ttattaaaag
       61 gtatttttta ttttaacata tatttaataa ggctaaatag agttattatt ttaatttcat
      121 actttacttt ttctataaac ctattataat taaagtataa tcatttttgc aacttgttta
      181 ttttattata aaaatgtgaa acttattaca taaggaggaa tttaacatga aaaatcatac
      241 ttataaagaa atcaaaaaca tatatggaaa aataagccct tttgaattta aggacaaatt
      301 aatagatatt gcaaaataca ctgcaaaaga aaataacaga gaattacttg atgcaggtcg
      361 tggaaatcca aactggactt gttcaactgc tagagaagcc ttttttactt ttggtcattt
      421 cgctattact gaaactcgtt ctaattggga tttaggtcat ttggctggaa tgcctcaaaa
      481 aaagggtatt aaagaaagat tttttaaatt tataaatgaa aatatagata tgcctggagc
      541 atatttagct agagatatta taaactttgg tataaatgaa cttggatttg atggagatga
      601 atttgttcat gaactagctg atggaattat tggtgataac tatccacttc cagatagaat
      661 gttacctcat atggaaaaaa tagtacacga ttacctagtt caagaaatga aatatgatat
      721 atcaggtaaa tatggtgatg tagaaatttt tgctgttgaa ggtgctacag ctgctatgtg
      781 ctatatattt gattcattaa tggcaaatga acttctaaaa aaaggagata ccatagcctt
      841 aatgactcca atatttacac cttatcttga aatacctaac cttcctcgtt acgactttaa
      901 agtggtaaat ataaatgcta atgaagttga tgaaaaaggt gctcatacat ggcaatatac
      961 aaaaaaagaa ttagagaaac ttcgtgacaa atctattaaa gctttattcg ttgttaatcc
     1021 taataatcct gcttctatag ctatggatga aacatcttgt aataatctaa tagatgtaat
     1081 tgaaaattat aataaagatt taatgataat atcagatgat gtatatggaa cttttgtaga
     1141 agatttttca tccttaatgt caaaactacc ttacaacact gttggagtat attcttattc
     1201 aaaatatttt ggagttacag gatggagact tggtacattt gcccttcata agaaaaatgt
     1261 ttttgataag aaaataaatg atttaactgg agaattaaaa aaatcagtag ataagcgtta
     1321 cagtgatatg agtcttaacc cttctagcct ttcatttatg gaaagagttg ttgctgatag
     1381 cagacttgtt gctctcaacc atactgctgg tctttctact ccacagcaag tacaaatggc
     1441 gtttttctct gcttttgcat tgattgacaa agtagatgcc tacaaaaaac tcaatatgag
     1501 tatctgccat agacgtcaaa aactattatt tgaagcttta gagcttccaa ttaatgaaaa
     1561 caaaaacaat gctgcatatt acactcaatt tgatattgaa gaatgggcta aattaaacta
     1621 cggagaagac ttatttaaat ttataagtaa aactgcatct ccagtagatg ttctttataa
     1681 attagctaat gactattcag tagttttact tagtggaaat ggattctatg gtcctgaatg
     1741 gtcaattaga atatctctag caaatctata tgatgaagct tatactaaga taggaaaagc
     1801 cataagagag atattaaatg gttatatcgt tgaatggcaa aaaattaaga aataattata
     1861 ttacactgaa atatctaaat tatatgaata aaagagttta tgtagacctt aactactttt
     1921 tttgatataa tcctatcgga attataaatt ttatttatga aaggcagaga atagtatgag
     1981 tattaagaaa aaagttgcca tagtttttac tggtggaact atatctatga cagtagatga
     2041 taaagtcgga gcagctatac caactttatc aggtgaacaa atactatcaa tggttaccaa
     2101 cattgataaa gttgcagatg ttgaagtttt taattttgat gaaattccag gacctcacat
     2161 aacaccttat agaatgatgg aattgaaaaa ttatgtaaat gatttattag caagaaaaga
     2221 tattacaggt gttgtaataa cacatggtac agatagttta gaggaaacag cttattttct
     2281 agaccttaca attgacaaca tcaaacctgt aatagtcaca ggtgctatga gaagtagttc
     2341 tgaacttgga tatgatggtt caagtaattt atctgcatca gtttgtactg cagtatccaa
     2401 agatgctatg ggaaaaggag tccttgtagt tttaaacaat gaagtattat tagcttctga
     2461 agctactaaa acaaatactc tttctctaaa tacatttaaa tctctaacta gtggcccatt
     2521 aggaataatt gactgcaacg aacttgtcct tacaagagat attgtaaata gaacaattat
     2581 agatactgat aaggtagaat ctaaagttgc cttatttaaa gcatatgtag gagaaaatgc
     2641 tgactttata agatttgctg ttgatagtgg ttataaaggt atagttattg aggcaatggg
     2701 tagaggtaat attcctcctc aaatgctttc tggagttgaa tatgctagag aaaaaggact
     2761 tcctgtagtt atagtttcta gatgtcattc aggtagagta ttcgatagtt atggttactt
     2821 tggttctggt agagatttaa aaaatcttgg ttgtatattt ggtggggact taccaggtca
     2881 aaaagctaga ataaaactta tcttagcact tggaaaaact gataaccttg atgaaatcaa
     2941 agatttcttt gaaaaaggaa tctattgtta attctaataa cttctattta attatttatt
     3001 gatatttcaa catttttaaa tcaaaatcta atacttatgt cagttaaatg tcagcaaaat
     3061 attttatcca caagttttcc acaacttatc cccaatatta tccacaagaa aaaaggtagc
     3121 acttatatta gtagctacct tttatttatt aatcttcatc tttccagctt tttatccaat
     3181 ttcctatttc tattattttt tcttcagtta tttcttctcc atcatgcaat tttgaaatta
     3241 attctgataa tggattgtta tgtagacctc caaaaattct ttttccctct attttgagat
     3301 attctttctc ttttattaat actctatagt gtacatgttt cccaattctc tgcgatatga
     3361 aaaaaccttt tttagttaat ctagctaaaa gtgtaattgt agttgtatgc ttccaagcat
     3421 atgttttttc tatttcatct gcaacatctt ttgatattac agttttataa ccagtattct
     3481 atatgtattt cataattttt aattctgcat ctggtaattt tttattcaac ataatatatt
     3541 ccttctttca atttatgtca cttcctcaaa cttatctagc ttaaattaat aataggagct
     3601 ttttatcaca aaaactacag gtaaattttt ctatatattc tacgtattta gtctgttttt
     3661 taagtgtgaa attacgttca tacctataaa tttattcaat tttcaaacaa tcttattgaa
     3721 taataactaa ttctattgta ttatttagtt atagaataaa actaaatcgg caaaactaga
     3781 gaaatctagt gacgcaaagc tatag
//
LOCUS       DAYAOX010000279         3774 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.282, whole genome shotgun sequence.
ACCESSION   DAYAOX010000279 DAYAOX010000000
VERSION     DAYAOX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.282"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            104..502
                     /locus_tag="ACGI57_003439"
     CDS             104..502
                     /locus_tag="ACGI57_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718486.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            complement(625..1647)
                     /gene="ccpA"
                     /locus_tag="ACGI57_003440"
     CDS             complement(625..1647)
                     /gene="ccpA"
                     /locus_tag="ACGI57_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HFG9718487.1"
                     /translation="MKMKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEV
                     IKETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANT
                     YSDKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYT
                     VSTSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKS
                     LIKYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVA
                     GFNDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRES
                     VTERKK"
     gene            complement(1894..2094)
                     /locus_tag="ACGI57_003441"
     CDS             complement(1894..2094)
                     /locus_tag="ACGI57_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718488.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(2128..2307)
                     /locus_tag="ACGI57_003442"
     CDS             complement(2128..2307)
                     /locus_tag="ACGI57_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718489.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(2489..2695)
                     /locus_tag="ACGI57_003443"
     CDS             complement(2489..2695)
                     /locus_tag="ACGI57_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718490.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(2743..3168)
                     /locus_tag="ACGI57_003444"
     CDS             complement(2743..3168)
                     /locus_tag="ACGI57_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718491.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            3340..3567
                     /gene="acpP"
                     /locus_tag="ACGI57_003445"
     CDS             3340..3567
                     /gene="acpP"
                     /locus_tag="ACGI57_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFG9718492.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
BASE COUNT         1337 a          634 c          409 g         1394 t
ORIGIN      
        1 tatttatatt atatatcact cacaattcac cacttcaata ttatttctat atataaatag
       61 tatgaatata attcaatata tatttataga aaggggtttt tttatgaaaa aattagatac
      121 agagtcatta gaaaaaatgg ctaaacaaaa aaatattgac aaggataaaa tagaaaagat
      181 ggctgatagt tacaaaggaa aatcagaaaa tgaacttatg gaggaactta taaaaatagg
      241 taagaaccta gatggcagag atgaagtagt atcaaaattc aaagcatttt tagatgaaaa
      301 tcaacgcaaa aaactggata atataatgga aaaaatctca gatgcagaaa accaaagaga
      361 tactaaacca gctaaaacta agaaggctaa acctactaaa ggtaagtcaa attcacctgc
      421 accaaaaaat acaccaggta gtcaacaaaa aaaatctaaa agtttattta agaaaactaa
      481 aaagtctaac cctcatgaat agataaaaat aaactattta agtaattaga taattatttt
      541 tagttaatct attattaaat ttgatattat tgacataaaa aatcaggtag tgccttaggc
      601 actacctgat tgattttaat agttttattt ttttctttct gtaacacttt ctctatcaac
      661 aattctataa ggtaattcta ttttcttatt ctcaattaat tccttattta ttaactttat
      721 aaccattctt attgctactg ctcccatatc gtatagaggt tgatatactg tagtaagttt
      781 aggtctatac attctagcta tcttaacatc attaaatcct gcaacagata tgtcttctgg
      841 aaccttatat ccagcatcac atatagcatt tatagcacct atggcagcct catcacctgt
      901 tacaaaagcc gcatgaggta ttattccatc atctaatagt tctttcatac tattgtatcc
      961 actctcataa tcagttccac catacttaat caaactcttg tctaattcta tattgttatt
     1021 tgaaagtgct ttctcataac cagcaagtct ttccatttct aaaacagtat catctttact
     1081 cgtcattata aaagctatct tttcatgacc tttctttata agatgctctg tcatatcaaa
     1141 agtagcttct tcgttgctag tacttactgt atatatatca taatttctag cagttttact
     1201 tatatatgtc gctggtattc cacaattttg tatatagttg atatgttctt gttcaactat
     1261 ccatgaaatc ataactatac cttccacttg ttttgctctc aatagattta tactcttaag
     1321 ttcttgttcc ttatcagagt atgtattcgc taaaagaata tcatagtcat acattttagc
     1381 aacctcttct atcccattta acacctcatt aacaaaagaa tctgaaactt ctggaactat
     1441 tacccctatt aattgactct tctttgttac taagcttcta gcaagtggat ttggtatata
     1501 tccagtctct tttataacct ctaaaacttt ttgtttgact tcatcagtta caggttttga
     1561 atcatttata actctagata cagtagatat tgacactcct gcttgtttag caacatcttt
     1621 tatcgttata ttgcctttca tcttcatcct ccttatttta ctaaatttat ctaaaactga
     1681 accaaatttt tgaaggttcc tattttaact acatttatat aagtttttat tttaccataa
     1741 aagcaagtac atttaaatac attatatttt atattatacc ctatattcaa caaatttaat
     1801 aatattcatt cttaatttaa atcatattaa aaaataaaat aaaaaaatgc tggtataaaa
     1861 taccagcatt tttttattct aatgatttaa gtattagcag ccgcaaccta cttcaccatc
     1921 aaagaatcca ccacatgaac aagttattag caataatatt aagaaaggta tccatgacat
     1981 tatgtcaatg tctctccagc aatcttcaaa agctaagaat aggaagaata gtactattat
     2041 ccaccatcct cctccaccaa agcatccttc ttctccaaaa aatctatcta acataacaaa
     2101 gccctccttt ttttaataac tgtgttctta ttctaagcat ccaccacagc agcaaagtat
     2161 taataaaact attaggaatg gtatccatgc cattatgtca aattctgccc agctatctcc
     2221 aaaagctaag aataggaaga atagtactat tatccaccat cctcctccac caaaacatcc
     2281 ttcttctcca aagaatctat ctaacattat acgttacctc ctgttgatat tttgttgagc
     2341 ttttcgctcc taacatatac tatgcatttg gtttccattt tgctaatgta tttttactat
     2401 ttttttaaat tttttaattt gaactaaaaa aggtaaaacc ataacttgta taagttactg
     2461 gttttaccta tttaataatc aatatttttt agcaacaaca accatcatcg aagttaaata
     2521 ataacaatac tattaatata acccatatcc atatattgtc atcgcataat aagtcttcta
     2581 aaatgtctgt gcataatagt aagaatacta taactattat tatcaacatt ttattcccaa
     2641 aaaatccttc gcatcctcca aagaaacgtt ccatattgtc ttctaatttt gtcataacta
     2701 atcctccttg aatcattaag agactaagtt ttgtctcttt aattaaatat atgtagtaat
     2761 agacagataa gttacttcta aaaagttttt attgattagt ttatttattt ctttttcacc
     2821 aagtttattg tatctatcaa cttcatccag ataatctttt ttctcctctg atatagtata
     2881 gattaaactt tctattgatt ttattttgta ctccttatat ctttctatca agaaggatgt
     2941 ttttgaattt tctattcctt tctttacatg cattaacctt gatatgtagt cttctttttc
     3001 attagataca taactctcct tttcagctac atcttcaagg attaaattgg tctccattac
     3061 ataatcctct atacgttgca tatctaaagt tattgaatta taataaaaat ttactgctat
     3121 aagtaaaaac attcctactg aagttagtaa tagtcctaca tatttcatag catttcctcc
     3181 taagttatat ttgttatcaa taaagtttat tcatcaaata atactttatt actacattta
     3241 taaaatttta ttatttttta tagattccat attgaaaaat tcttgatatt atattaaact
     3301 aacattaaaa ttaattaaaa ctgctttagg aggcttaaaa tgtttgagaa aataaaagaa
     3361 ataatagcag aacaattagg ggtagacaat ttagatgaga taactatgga agcatcttta
     3421 atggacgact tagaagctga ttcattagat gctgttgaaa ttataatggc ccttgaagat
     3481 gaatttggaa tagaaatacc agacgaggaa gctgagaact ttaaatgtat tggtgatata
     3541 tgtaaatata tcgaagaaaa caaatagtat cgttttgtct acaaatataa atagtctgtg
     3601 taaacttcta cataataaat tatattagag ttcgcacaga ctagattaca tttatttaca
     3661 atatataaaa tacacgctct tatatttaat ttatatacta agtatataaa ttacttttct
     3721 tcataaaacg taacattctt tattattata tctatagttc catcttgatt ttgt
//
LOCUS       DAYAOX010000280         3752 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.283, whole genome shotgun sequence.
ACCESSION   DAYAOX010000280 DAYAOX010000000
VERSION     DAYAOX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.283"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..891
                     /locus_tag="ACGI57_003446"
     CDS             <1..891
                     /locus_tag="ACGI57_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718493.1"
                     /translation="ELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNNN
                     YEELTSKESVYYVSDSYKKIFFKDFEVEKINLHIKDTNEYDINLFKVQKSILEPEINS
                     QATFDLIVAKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYPF
                     NFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLIK
                     LGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIYF
                     IGYFIYYFLTLKYALNMIKE"
     gene            1116..2729
                     /gene="ggt"
                     /locus_tag="ACGI57_003447"
     CDS             1116..2729
                     /gene="ggt"
                     /locus_tag="ACGI57_003447"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFG9718494.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2862..3713)
                     /locus_tag="ACGI57_003448"
     CDS             complement(2862..3713)
                     /locus_tag="ACGI57_003448"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HFG9718495.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
BASE COUNT         1451 a          470 c          599 g         1232 t
ORIGIN      
        1 gaacttatag acaaagatat aaatattgaa atgtattata aaaatggaaa tagttatata
       61 gaagatagaa cgtctgtaga tattattaga gaaagtgatt ttaaaaaatt aagacaatat
      121 cagaataaca attatgaaga attaacttca aaggaatctg tgtactatgt ttctgattca
      181 tacaaaaaaa tattttttaa agactttgag gttgaaaaaa taaacttgca tattaaagat
      241 acaaatgagt atgatataaa tctgtttaaa gtacaaaaaa gcatcttgga gccagaaata
      301 aattcacaag ctacgtttga tttaattgta gcaaaagatg acgtatttaa tcagttaaaa
      361 ttacatggaa attctagatt aataagatta attgatataa aaaatgaaaa aaaagaactg
      421 gatttgactt taaaattaaa aaatatagta agtgaaaata tgaaatttac ttatcctttt
      481 aattttacat caagtattga aagttataat aacttgataa aattaagtgg tctaatgctg
      541 tttataggca tatttttgtc tgtagtattt cttttatgta caggtagtat aatcttattt
      601 aaacaattat caaatatata tgatgataag gaaagatata ttatgttaat aaaacttgga
      661 gcaaataata aagatattga gaaaataata tcaaaacagt taaaagttat atttttaatg
      721 ccattagttg taggtacagt tcataattta tttgctatgt ctatagcaca aaagtttata
      781 ccaaggtcat tacttgtacc aattatcata actctagtca tttattttat aggatatttt
      841 atatattatt ttttaacttt aaaatatgct ctaaacatga taaaagaata aattattttt
      901 aagtcaaaag aattagatat atgttttgaa taaaataact tatttaagtg cagatagaaa
      961 atactagaat tgagtttaag atatttattt aaagacaaaa ttaaagttag gaaaaagtct
     1021 atattttata tcattttgta tcagttacat tacttgatgt tatataataa atctaagcag
     1081 aatatcaaat aaaaaatgac gaggagctga aatttatgaa gtttaatgcc taccaacata
     1141 aatactcatc aataagaaat gtcgtgtatg ctaaaaatgg agcagttgca acatcaacac
     1201 cattagcatc tcaagctgga cttgaaatac taaaaaaggg tggaaatgct gttgatgcag
     1261 cagttgcaac agccgccaca ttagcagtag tagaacctac aagcaatggg attggaggag
     1321 acgcatatgc ccttgtttgg atagaggaag aaaaacgatt gtatggttta aattctagtg
     1381 gctttgctcc agaaaatatg gaattaaaaa attataataa tatgaaagaa atgccaaaat
     1441 atgggtttgg agcagtaact gtacctggaa taccagcagc ttggtcagaa ttaaataaaa
     1501 aatatggtaa acttagctta atggaatgct tatctccagc gataaactat gcaagagagg
     1561 gatacgtagt atctcctaat gtagctaagg tatggaaaaa atcatatgaa ctctatgaaa
     1621 aagaacttat tggagaagag ttcaaaccat ggtttgatac attttctaaa gatggtaaag
     1681 ctccagaggc tggagatata tttatatgtg aagaacaggc aagtacatta gaagagatag
     1741 caaatactca ggctgaaagc ttttatagag gaagacttgc tgataaaata gatgaatatt
     1801 ctaaaaaatt taatggggct ataagaaaga gtgacttaga gtgtttttat ccaacatggg
     1861 tagaacctat aagtacagaa tataagggat ataaaatatt tgaaatccca ccaaatggtc
     1921 atggaataac agttttaatg gcacttaaca tattaaaaga attggaatta gaaggaaata
     1981 tagaaaatgt agaagacata cataaaatta tagagagttt aaaattagct tttgcagatt
     2041 ctaaaacata tgtaactgat atagagcata tgaaggtaaa gattcaggaa ttactaagcc
     2101 aagagtatgc taaaaaaaga agtttattga ttgacaataa agaggctttg tatccaactg
     2161 caggagagcc atattgtggt ggtacagtat acctttgtac tgcagataag gatggaaata
     2221 tggtttcata tatacagagc aattacataa attttggttc aggaattgtg ataccaagaa
     2281 ctgggattgc tttacatagt agaggaaata attttaattt agaccctaaa caccacaatg
     2341 tagtaaaacc atttaaaaaa ccttatcata ctataatacc tggattttta ggtaaagaag
     2401 ataaagcaat aggtccattt ggtgtaatgg gagcttttat gcaaccacaa ggacatattc
     2461 aagtgttaac aaatatgatt gattttggat taaatccaca agaggcgcta gatgctccaa
     2521 gatggcagtg gataaaagga aaagaaatag aagttgaacc agagatgcca aagcatataa
     2581 tagattcatt aattgaaaag ggtcatgaaa taaaagtaat tcatgatact gtcgacatgg
     2641 gacgaggtca aataatattt aaaacagaac aggaaagtta tatttgtggg acggaatcaa
     2701 gatgtgatgg tcatattgca gtttattaat attatcaact aaaatatata taataaattt
     2761 aaagagtata tgaaataata agtaaaggcg aaagtctaca atgactgacg cctttacaaa
     2821 atttaagcta tcatatgctg attttatata gattatgagc cttattcttg accagttttg
     2881 atttcattgt catcataact aatccagtca ctaaagctac caatataaat cttgtgaggt
     2941 atatccagtt ctctaagagc aagactgtta acacaagcag atatccctga accacaagat
     3001 aaaattactt catcataatt atttaaatct ttaaagaatt tttttaaaaa ttctatatct
     3061 ttaagtgagc cattttcaaa gtcacttttt attaaatcct tacaaaaata attttttgca
     3121 cttggtatat gaccagcttt tgagtaagca ggttcaacta aaccctggta tctctcatta
     3181 gcacggcaat ctattattac agtatctttt ttgtataatt tagatttaac atattccatt
     3241 ggaacaacta aatcattgtt tatatttggt ctaatttcct tacctgtaca acttggtata
     3301 ttcactttct cttccaattc tccaccttcc tttacgaaag atgttattcc cccatctagt
     3361 acatagacat ttttaagccc caaatgtttt aattggaaaa ataatctgca agctccattt
     3421 aaatctccat catcataagt tacaatcgta gtgtcattat caacacccat tttttctaat
     3481 ttttctttta gtattaaagg gtcttgtaaa ggatttttac caccatgttc ttttgtagga
     3541 gatgataaat ccttgtctat atctaagata aaactgcctt tgatgtgtcc ttttttataa
     3601 gaatcaatac cataagttct attgataaga tcaaatctac aatctattac aactagatta
     3661 tcattttttt ctagttttga tataagttct tttgctgaaa taatgttttt caaagaatgt
     3721 acctcctgtg tttatattat ctttatttta at
//
LOCUS       DAYAOX010000281         3747 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.284, whole genome shotgun sequence.
ACCESSION   DAYAOX010000281 DAYAOX010000000
VERSION     DAYAOX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.284"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            27..695
                     /locus_tag="ACGI57_003449"
     CDS             27..695
                     /locus_tag="ACGI57_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFG9718496.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            854..1360
                     /locus_tag="ACGI57_003450"
     CDS             854..1360
                     /locus_tag="ACGI57_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFG9718497.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1488..2504
                     /locus_tag="ACGI57_003451"
     CDS             1488..2504
                     /locus_tag="ACGI57_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFG9718498.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTPANKKNKSSKTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     regulatory      2700..2794
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            2858..3562
                     /locus_tag="ACGI57_003452"
     CDS             2858..3562
                     /locus_tag="ACGI57_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HFG9718499.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1604 a          321 c          656 g         1166 t
ORIGIN      
        1 ataaattttt taaagggtga ttttttatgg aaaatataac tagttatttt acaacaatac
       61 tatgtgtatt tatatgctta tcatctgtat ttatatttac tcaactagct agagtattta
      121 ttaataagaa gaagattaat caaaaaatta aatcaagaaa cggatttagg tatgatagag
      181 attttattga agccagaaga gaagaaatac atataaagga taacaataat aataaaaata
      241 agagtaacaa taagaaatta aagggagaaa aagtatttaa atacgataat ggtgatttat
      301 ataaaggtga gtttgttgat ggaaagaaaa atggatttgg gatatatatt ttttcaagta
      361 aggaaaagta tgaaggatta tggaaagatg acaagatgca tggaatagga aaatatactt
      421 atagagatgg ttcaatatat acaggtgaat ttaaatacgg attaaaaaat ggtttgggaa
      481 aattaacata tcccaataat gacatttata agggatattt tttagataat aagaaaaatg
      541 gtaagggtgt attatataaa aatgatggaa ataaacaagc aggaatatgg gaagatgatg
      601 aacaatgtaa aagtttagat tttaaagatt taaataataa taaaataaat aatgtttatc
      661 aaaatagatt aaataacaat aaattaaaag tttaattatt ctaataaaaa aatctttatt
      721 tatgatggaa tataggatat atctatgttg taatttgtaa aatcttccta tatcatataa
      781 aattaagtat tgattagtct attacataaa tatagaatat aaatatatcc atttaataat
      841 aaggagaaga gatatgaaag agtatgtagt tgtggattta gaaactacag gattagatcc
      901 atataaaggt tgtgaaataa tagaaatagg aataacagaa ataaaaaatg aacaaattgt
      961 aaaaaactat tcaaggttga taaaacctaa ggggataatt tcttcattta taacagaatt
     1021 gacacatata agtaatgaaa tggtagaaaa tgaagaacca ctagaattag ttttacctag
     1081 atttagaaaa tacataggtg atagaactat aattgcacac aatgcgaaat ttgatttaaa
     1141 atttttaaat tactatctta gaatgttaga tttagaacca ataaacaatt atatatgtac
     1201 agtagagtta ttaaaaaaat gtaaaagtta taaaggaaaa aacaagaaac ttgaaacagc
     1261 ttgtgcttat tacaacatag agaatataaa tgctcataga gctgacagtg atacattagc
     1321 aactgcaaaa ctatttttga aaataaaaga tgaatattaa ttataaatat agagaaaatt
     1381 agtgaaaata tcctttataa aattgaccct taagcttaca attttgttct ttgatttatt
     1441 ttgtagaaca atgtataatg taccttatat gaagaaggga gactaaaatg ggttattaca
     1501 atgataatga taataataaa ataagacgta aaaaaattag taacagcaaa gatagtaata
     1561 aaaaatctaa acattcttat actccggcaa ataaaaaaaa taaaagttct aagacaagtt
     1621 ctagtaaaag aatgagtgaa aaagtagatt cacagaatct tataaaaaga gatagagata
     1681 gttttacaag agataatgta agacctttaa atacagatta caatcaaaga gaaagaagaa
     1741 gagtcgagta taaacaaaga gagaaaaaat taaaaagtaa aaaaagaaaa aaacaaaaaa
     1801 ggattagaat agttagagca ttattatcta ttatgttagt tgcactaatt atattttcag
     1861 tggcaggtgc aatatttaca gtttcagcta taaaagggtc accagaagtt actaaaaagt
     1921 taattaggtc taaatatata agtagtgagg ttgtaagtat aaaacaaatg ccaaatgatt
     1981 taaaacatgc aatagtttcc atagaagatg agagatttta taagcataaa ggagtagatg
     2041 ttatttctct agttaggtca gtattaaaca atgtgtttac agatacaaca caaggtgcaa
     2101 gtactataga tatgcaagtt tcaaaaaacc tgcttactag taatgataaa actatgaagc
     2161 ggaaaataag ggatatgtat aatgctaagc agatgaataa gattatgact aaggatgaaa
     2221 ttcttgaagc atatttaaat aatatgtatc taggtaaaaa tgcatatgga gctgcaaagg
     2281 gagctgaagt ctactttggt aaaaaagtta gtgaattaaa tttagctgag tgtgctatgt
     2341 tagctggtat gacaaataat cctgcaaggt atattgacca tggagaggct aagaagagac
     2401 aagtgattgt actgtataag atgcatcagc ttggttatat aactgataag gagtatagaa
     2461 tagcaaaagc tgatgacact ccatttaaat cagaaattga ttaaaattgt aagaatatag
     2521 tattttcaat agtttaaaca aacaaaaatg taataaaaaa ggtgatttgc cacccatttg
     2581 ccaccataat tataatctaa tggcaaattg ttttaatgta caaactagat aatagaatat
     2641 tgtcttaaat gctatattat gctaaaattt aattgtattt tgtaaattaa aaaacttata
     2701 atttagtttt ctagggttcc gcattttata atggtcaggt ccaagagaaa acgcgcaatt
     2761 gttgtgtaca cggaaggata aaagcctggg agatatttaa gaatatttct ataggctttt
     2821 ttatttttac atattttaaa tttgaggagg ttttctaatg aaaaaatttg ttattgagga
     2881 tgcattttgg aatctatttc ctagtgcaaa aattggagtt gttatttgta atgatataga
     2941 taactctata aaagatgaag actataagaa aataattttg aagggagaag aagaagcatt
     3001 aaaatacttg gaggactcag aatttagcaa caaccaagtg attaaagtat ggagagaagc
     3061 atttaaaaaa tttaaaacga aaaaaggagc aaggtcttct attgaagcat tattaaaaag
     3121 agttcataat ggaaataata taggaactat taacccttta gtggatatct acaattttat
     3181 ctcattaaaa tatgcattac cttgtggagg ggaggatata gataaattta taggagatat
     3241 taggctaact agagcaattg gtaatgagga gtttatccca ctagggacag atgaaaattc
     3301 atttccatat gaaggagaaa taatttataa agatgatggt ggagcaattt gtagatgttg
     3361 gaattggcgt gaagcagtga gaacaatgct tactgaagat actaacaatg cctttttatg
     3421 cattgaatta gtagatgaaa gtagatttaa agaatttgaa aatgctttaa atgaattagc
     3481 taaaatagtt caagaaaaat tgggtgggaa ttgtaaaatt tcaattttag atattgataa
     3541 taaagaggta tcagtggatt aatatggagg tgctgttgta atggactatt ctagttataa
     3601 agatacttat atttagggta agaaggtcta ttttagatat aacgattatc aaaggatata
     3661 atatagaaaa taaaaatgta gaatataaaa agcctttaaa ataagaaaaa aatgtatttt
     3721 aaaggctttt attatactct tttttat
//
LOCUS       DAYAOX010000282         3734 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.285, whole genome shotgun sequence.
ACCESSION   DAYAOX010000282 DAYAOX010000000
VERSION     DAYAOX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.285"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..931
                     /locus_tag="ACGI57_003453"
     CDS             <1..931
                     /locus_tag="ACGI57_003453"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9718500.1"
                     /translation="IIIAGFIYWYSSKKFNIKVMNFDKYIAILMIPLLLIILFMQSFE
                     YSSNINIDTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFTFKKLIQALEDDKE
                     RAIMNQQIHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKKDQILKAQDYLNKL
                     EKVTSDLSFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQSTNMNDFDLCIVFA
                     NAIDNAIKACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNYTSGSGIGLLNIKE
                     IAKKYQGAISIDKTSDYFQINVLLVISLHCASI"
     gene            1021..1455
                     /locus_tag="ACGI57_003454"
     CDS             1021..1455
                     /locus_tag="ACGI57_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718501.1"
                     /translation="MKVGNINGQAPITNGLDSCQSEVQGLEQKKKALQDEIEKVRSDK
                     SIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEENNVSEVNNNLLERKKEPS
                     NNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRPI"
     gene            complement(1718..1954)
                     /locus_tag="ACGI57_003455"
     CDS             complement(1718..1954)
                     /locus_tag="ACGI57_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718502.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTYKIQTVS"
     gene            complement(2110..2334)
                     /locus_tag="ACGI57_003456"
     CDS             complement(2110..2334)
                     /locus_tag="ACGI57_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9718503.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2431..3684)
                     /locus_tag="ACGI57_003457"
     CDS             complement(2431..3684)
                     /locus_tag="ACGI57_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFG9718504.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
BASE COUNT         1481 a          483 c          484 g         1286 t
ORIGIN      
        1 tattataata gctggattta tatattggta tagttctaaa aagtttaata ttaaggtcat
       61 gaactttgat aaatatatag caattttgat gattccattg ttgcttataa tattatttat
      121 gcaatcattt gaatatagtt caaatataaa tatagataca agtcttggga ttgtaaagct
      181 gctattaaat acttctataa ctgaagaaat tcaagctttt cttttttcta taattggaac
      241 tatatgtgta tttttttctc tatttacctt taagaaacta attcaagctc tcgaagatga
      301 caaggagagg gcaattatga atcaacagat tcatgctcaa aaaaactata ttgaagaagc
      361 gaaatcacga ttatcacaaa ctatatcctt tagacatgat tttaataatc atttagctat
      421 tgtcaatgga ttgttgaaaa aagaccaaat tttaaaagca caagattatt taaataagct
      481 tgaaaaagta acaagtgacc tatcattttc ttgtaataca cagaatattg ttattgatgc
      541 tcttttcaac aataaattaa gtatagcaaa acaattaggc atagaggtag attgtgaagt
      601 aattataccc caaagcacta acatgaacga ttttgaccta tgtatagtgt ttgctaatgc
      661 tattgacaat gcaattaaag cttgtagagt tgtagataaa caaaataggt atttaaagct
      721 ttccactaag ttagagggtg gattttttat gatagaaata aaaaatagct ataatccaat
      781 aaataattat acaagtggct caggtatagg tcttttaaat ataaaagaaa ttgctaaaaa
      841 atatcaaggg gctataagta tagataagac atcagactat tttcaaataa atgtattgct
      901 agttatttca ttacattgcg ctagtatttg agaacaattt cattgaaaat agtatttgta
      961 agctatatta tgtatttgaa aagagaaagt ttgatatata gaaatgagga ggttactaaa
     1021 atgaaagttg gaaatataaa tggtcaagca ccaattacaa atggtttaga tagttgtcaa
     1081 agtgaggtac aaggcttaga acaaaagaaa aaagcattac aggatgaaat agagaaagta
     1141 agaagtgata aaagtattga tggtgaaacg gccaaattaa aaatagaagc tttaaaaaaa
     1201 gaaatggaag agattgaaaa taaaattcaa gagttaaaag gaaataaagg aaaaaaaaca
     1261 aataaagaag aaaataacgt ttcagaagta aataataatc tattagagag aaaaaaagaa
     1321 ccttctaata atgaaaatgt aaaacaatta tatgatgttt ttgaaagaag caatgtagac
     1381 gaacaatggg ataatactta tcgtgttctc caagatgata aaaacttaaa agtaaaattt
     1441 aatagaccta tatagtttat ttataaaaat agttaatatt ttaattatca ccacttttta
     1501 gaagaataca cactttattt taaagaccca catatttatt tacagttagc acatttatat
     1561 gtggttttat aactattatt tacaagtcta tattgataac ttttacatag tatatgatgt
     1621 ttaaatactt attcaaattt atctatacat aaacaaaaaa agagatttta aaatctcttt
     1681 ttagattgtt gacttatggc tttttgattt aataaaatca agaaaccgtt tgaattttat
     1741 atgttggctg atacccttct gatattgaaa caccattttt gtttccaaaa tcgtttaaaa
     1801 ttaagaactt attatgaatg actatttcat tatgaacaat aagcaaataa catgaatctt
     1861 caatcaatgt tttaacagta gcattaaatg cttgcttata tagttctatc gtttgtttga
     1921 actgcccatt aaagtaaata tgagtcaata acataactat atctacacaa attaatataa
     1981 attttttata ctgtacatta tctaactgtt gattatctgt attacaacat gattgaattt
     2041 aaaaagccat caacacatag aatgttgatg gcctgaaaat agattttaaa atctcttttt
     2101 tattatatat taattatttt ttaaatttat attttttgca tcagattttc taatagctaa
     2161 gtcaaaccct ctaaatctaa tcactatagg gtcattaaga ggagctatct ttttcacttc
     2221 aacttcagtg ccatctatta gtcctaaggc tagaagtctc ttcataagtt tttcatttcc
     2281 agctatattg tctataattc ccttttgtcc aagttttaag ttgtaaacag tcattattaa
     2341 cacctcctaa tgaaaatcat tatcaaactt tatatttata atatataaca aaaagagcaa
     2401 attgtcaata tataacaaaa aataaacata ctaagttagt atgtttataa aaaatagtat
     2461 acatgggata ctaatagcat ctagcaaaaa accacttact aatggtacaa ctaaaaaggc
     2521 ttttggagaa tcaccatatt tttgtgtcaa agaactcata tttgccagag cgttcggagt
     2581 agctcctatt ccatgtccaa tcaaaccact tattatgatg gcagaatcaa aatcttttcc
     2641 aagtactcta aagcaaataa atattgaaaa taggattata aagattactt gaaaagttac
     2701 tattaagaga ataggaggta ataatgtaga taattcaaat aaatctatat tcataagtga
     2761 taaagttaaa aaaattccca atgaaagttc ttttaaaaaa tctataagtt taaaatttaa
     2821 ttctagtata tttatcttat catttaaatt tctaaaaata acagccgaaa acatacaagt
     2881 tacaacagag ggtaatagta tattaaacgt tatatagaag catctactta taatttctcc
     2941 tgtatttata caaataaata tgagaagaac ctgttccaaa aatacagatg gtgttattgt
     3001 ttcggagaag ttattatttt ttatcctatt cttatttcta aaaaatttac ttccaaatct
     3061 ctttagattt ctattatatc taggaatcga caagtctata tttgatgatg gttttaattt
     3121 atatttattt attagaaatt tagctacagg acatcccaat ataccaccca taataagtcc
     3181 tattgttgca gcagataatc caaccccaac tgctccttct ataccaagat tttctattgt
     3241 aagtccaaat gcagcagaat aaccatgacc accttccata gatacatttc cacacataag
     3301 ccctaaaaga ggttcaagtt ttataatctt tgatattgta atagctaata tattttgaca
     3361 aaatgccaat actccacaca aaatccaata tctaaataat acactaccac ctttctttat
     3421 aagatttatg cttatttcaa gtcctatagt tgtaaaaaag aaggttatga aatttggcat
     3481 taaatttcca cttatagata tatctgctat attaaaatat ttaaaaaata aatttagaaa
     3541 acaaaataat agacctccta ttactggtgc aggtatacag aaattattta aaaagttaac
     3601 tttattcttc aataaattac caattaaaaa gaagaatata gatagtgtca aggtttgcac
     3661 tatatcaaaa cttaaggtaa tcaaaataag tcctcctttg taattcaaag ttttaattat
     3721 ataacttaaa ttct
//
LOCUS       DAYAOX010000283         3682 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.286, whole genome shotgun sequence.
ACCESSION   DAYAOX010000283 DAYAOX010000000
VERSION     DAYAOX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.286"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            3..524
                     /locus_tag="ACGI57_003458"
     CDS             3..524
                     /locus_tag="ACGI57_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFG9718505.1"
                     /translation="MIVNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVDNKMT
                     AQDLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDKEISN
                     QELSDIENKMFIKEALTKLPELEFEVIILRYYHDLRFKDIAKIMESSVSVTKYRMSRA
                     LKKLELYLEGGKL"
     gene            521..1282
                     /locus_tag="ACGI57_003459"
     CDS             521..1282
                     /locus_tag="ACGI57_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718506.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICIFLFSTSADSNSFYNSLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            1320..2186
                     /locus_tag="ACGI57_003460"
     CDS             1320..2186
                     /locus_tag="ACGI57_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718507.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSIEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            2207..3448
                     /locus_tag="ACGI57_003461"
     CDS             2207..3448
                     /locus_tag="ACGI57_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFG9718508.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNITTDEGGFSNF
                     KPDIYWKFFYPNKAFYNFIIESYSNPNDYNPEIIKSMPLEDGVNFYKFRNEKVNTILN
                     MEYPDKNYTQKEKDFWLSKNKDIEEPYLNGYYQGWNYFLLARDLLIFTIVAVCISTAS
                     VFSGEYQSGADSIILSSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGID
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLIIYMILILLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFLKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLTVVFLPFIKNNFKKHQVN"
BASE COUNT         1476 a          390 c          543 g         1273 t
ORIGIN      
        1 tgataatagt gaatgatgct cggattatat cacaaataaa agcaggaaaa aaagaattgt
       61 ttgatactct aataaaaaaa tactatgata gcatctactg ttattgttat cgccatgtag
      121 acaataaaat gactgcacaa gatttaactc aagatgtatt tgtaacagtt ataaataata
      181 tagaaaatta taaacattat ggtaagtttg aaaactattt gtatgttatt gcaaaaaaca
      241 agtgtaagga tttttggaag aaaaaacaac caatatattt agagtcatta gataaggaga
      301 tatcaaatca agaattatct gatatagaaa ataaaatgtt tataaaagaa gctttgacta
      361 aattaccaga attagaattt gaagtgataa ttttacggta ttaccatgat ttaagattta
      421 aggatattgc taaaattatg gagtcaagcg tctctgtcac aaagtacagg atgagtaggg
      481 cattaaaaaa actagaatta tatctggaag gaggtaaatt atgaataata aagaaataaa
      541 aagaagtcta cagaagtata aggttcccaa atatgattct tcagctcttg aaaagactat
      601 taaaactata catgagttaa cgcttagtaa tacttataaa agaatgaata ataaagactt
      661 ttttatatct caaattggtt ttataagaaa aagaacttgg attatgcaat ttctaatact
      721 atgtacaata tgtatatttt tatttagtac atcagcagat agtaattcat tttataatag
      781 tttatccttg atttctatag catcaccact ttttgtactg acaaatatag aagaattaac
      841 aaaagtgtat aatagtagta ttttagagat agaattttca acaaaaaata cattacataa
      901 aattattgct actagaatgc ttatatttgg aataagtgat ttattgttta tgattattat
      961 tttggtatct gcaaaacaaa tcattgatgt aaacattctg tatataataa tttatatact
     1021 agttcctttt aacctaatgt gtataggatg tatggaaatt ataaatagaa ataaaggtaa
     1081 gcatacaaat tattattgta tgatatacgg tatatctctt gtattattaa tagttacact
     1141 gagtaacttt agagataatc tatatcaatt tgaagcaata aatcaatggc taattgtttt
     1201 tctagtcaca gcagtaacat taatatatga aataagaaaa acgtggagac atctttgttc
     1261 atttgaaaat attattatct aagtaaaaaa taagaataca cagtatcagg aggaatagta
     1321 tggagttaag tgtagaacgt ttaacaaaac agtataacaa taaaattata gtagataaaa
     1381 ttgactttaa attaaatgag ggtatatatg gatttttagg agaaaatgga gctggaaaaa
     1441 caactcttat gagaatgatt tgcggaatat taagtcctac atctggagaa gtaaaggtgg
     1501 atggtaaaaa taatttggat atgggagaag attttagaga tatgattgga taccttcctc
     1561 aaaactttgg atactaccca gattttactg ccaaagaatt tatgctatat atagcatcat
     1621 taaaaggatt aatgcctagc tatgcaaaga gaaaaacaca agaattatta gaaatggtag
     1681 gattatcaga ggtaattaat aaaaaaataa gaacattttc tggaggtatg aagcaaaggc
     1741 taggatttgc tcaaacacta cttaatgacc ccaaaatttt aatattagat gagccaacag
     1801 cagggctaga ccctaaagaa cgagttagat ttcgcaacct aatttcagaa ttttctaagg
     1861 aaaagattgt tatattgtct actcatattg tgtctgatgt agaatatatt gcagataaaa
     1921 ttttaataat gaaaaaagga agatttgttt taactggctc aatagaagaa gtaacttcaa
     1981 agataaaaga taaggtatgg aaatgtgtgg tcaatgagaa tgaaatagat tatatgtctg
     2041 atagatattg tttagcaaat atccatcatt ctggtggagt tgcaaatctt agaattgtaa
     2101 gtgatataaa gccacatgac aatgcatgca atttaaaacc aacattagaa gatttatatt
     2161 tatattgttt caacgaagta ctataaatat acataaggag ataggtatgt ctacattaat
     2221 taaatttgag ttaaaaaaaa taataaaaag aaagtctaca atagcagtta ttttaggaag
     2281 tttattctta agcatcattc tttttgcatt aatgccaatt caggaaaaag ctaatgatga
     2341 aagtggaaaa gagtataggg gtttacaggc tattgagcta aaaaagcaac aatctaaatt
     2401 aaatgaggga tacataacag ataaaaaagt aaaaaactca atcagagatt accaaaaaat
     2461 gtttaataat ccaaataata ttacaacaga tgaaggtggg ttttcaaatt ttaaaccaga
     2521 tatttattgg aaattcttct atccaaataa agctttttat aattttataa tagagtcata
     2581 tagtaatcca aatgattata atccagaaat aattaaatca atgcctttag aagatggagt
     2641 taatttttat aaatttagaa atgaaaaagt aaatacaata ttaaatatgg aatatcctga
     2701 taaaaattac acacagaaag agaaagattt ttggttgtct aaaaataagg atatagaaga
     2761 gccttattta aatggatatt atcaaggatg gaattatttt ctattggcta gagatttact
     2821 aatttttact atagtagctg tctgcataag tacagcttct gtattttcag gagaatacca
     2881 atccggtgct gattcaataa tattatcttc taaatatgga aaaacaaaag ttataacagc
     2941 taaaatagta agttcctttt tatttggtac gattattttt acaataaata caattgtgtc
     3001 tattggtata cttcttttat cttttggaat tgatggatgg aatttaccat tacaacttac
     3061 aaccagttca attccatacc catatacatt tttacaggca atattactaa gtgtgttaat
     3121 tatttatatg atattaatac tattaattag ttttacactt ttattatcat cttatatgaa
     3181 atcacctttt ccagttttaa ttacaaatat aatattacta tttgtaccaa tatttttaaa
     3241 atatagtgaa acaaataagt tgtataatca aattctcaat gttttaccat caaatgctat
     3301 ttcattttca tttactaatt atgtagatta taaatttggt aatttaataa tagctttacc
     3361 tacaatgatt atttttacat gcttagttct tacagttgtt tttttacctt ttattaaaaa
     3421 taattttaaa aaacatcaag ttaactaaaa tatatattat aaaaagaagg tgtcttaaaa
     3481 tgaacttttt agcttattag gcactcttct ttgtattaat ttaaatatat tttagtatta
     3541 gattaaatat gttttcatca ttttaacaat gaaaaagagc tttatctcta ttttgagata
     3601 gctcttataa agttaaatat taaattgata actataaaat atgtagtttg aatttataat
     3661 ttgtgttata atcatactga tt
//
LOCUS       DAYAOX010000284         3642 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.287, whole genome shotgun sequence.
ACCESSION   DAYAOX010000284 DAYAOX010000000
VERSION     DAYAOX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.287"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(126..1973)
                     /locus_tag="ACGI57_003462"
     CDS             complement(126..1973)
                     /locus_tag="ACGI57_003462"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DpnII family type II restriction endonuclease"
                     /protein_id="HFG9718509.1"
                     /translation="MKPILKWAGGKTQLLDILRKQMPTGCDFSSMTYIEPFVGAGALF
                     LYLLEEDIFNKYVINDINSKLINLYIKVRDDLEELITELTNLKEQYISYEEGSSDREK
                     MYYDIRDRFNILTNEKNIENEDCIKQASYFIFLNKTCFNGLYRENSRGEFNVPFGRYK
                     NPSFFDKNELEKLSMLLNKRDSRGELKVKILNKNYLNLENIIDKNCFVYLDPPYRPVT
                     IGGFNSYNKGNFNDDSQIELSKFYKVLDKIDTKLMLSNSDPKVLDEEDTFFDDLYSDF
                     NIKRVHAKRFINSKGNGRGFITELLITNYEEVGNQMELVKNIHKNKSKDEIFKELLEN
                     LKGTIASWDYYVNWNKALENTRKIEVQLNILNYLIGKEDIENETKNLLKEYPEVLSVI
                     PIVIASRDNEFKILDPSDEDMFKYKLFNFKKRKSISDEESELATEFLKKIKFLDLLKQ
                     KNIKNLVDYVFGVEVGLDTNARKNRTGSSMETLVERYVKNMCEKNNWSYLTQATVKKI
                     SEKWSIEVPTDKSKRKYDFAINNNGKIVLVEVNFYNGGGSKLKSVANEFTKLDKYIKD
                     GGFTFCWITDGLGWLESSIPLRDAFDKMDYIFNLDMVDKGSLEYIIKNL"
     gene            complement(2401..2750)
                     /gene="ssrA"
                     /locus_tag="ACGI57_003463"
     tmRNA           complement(2401..2750)
                     /gene="ssrA"
                     /locus_tag="ACGI57_003463"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2908..3363)
                     /gene="smpB"
                     /locus_tag="ACGI57_003464"
     CDS             complement(2908..3363)
                     /gene="smpB"
                     /locus_tag="ACGI57_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFG9718510.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         1205 a          649 c          342 g         1446 t
ORIGIN      
        1 ataaatatta ttgatatttt ataaatataa ttatacttat atctcatact caaatatttt
       61 atataatata taagtctaaa ttaacttgca atataaaagc gcctcttaat ataggcgcta
      121 taatgttata aatttttaat tatatattcc aatgaacctt tatcaaccat atctaaatta
      181 aatatataat ccatcttatc aaatgcatct cttaatggaa tacttgattc aagccatcct
      241 aacccatctg taatccaaca aaatgtaaaa cctccatctt ttatatactt atctaactta
      301 gtaaactcat ttgcaacaga ttttaactta gaacctccac cattataaaa attaacttct
      361 actaaaacaa tttttccatt attatttata gcaaagtcat actttctttt actcttatct
      421 gttggaactt ctatagacca tttctcactt atttttttca ctgtagcttg tgttaaataa
      481 ctccaattat tcttttcaca catattcttt acatatcttt ctacaagagt ttccatacta
      541 cttccagttc tattttttct agcattcgta tcaagcccaa cctctactcc aaacacataa
      601 tctactaaat tttttatatt tttttgtttt aataaatcta aaaactttat ttttttaaga
      661 aattctgtag ctaattcgct ttcttcatca cttatactct ttctcttctt aaaattaaac
      721 agtttatatt taaacatatc ctcatctgat ggatctagta ttttgaattc attatctcta
      781 ctcgctatta ctattggtat cacactaaga acttctggat actcttttaa taaattttta
      841 gtttcgtttt ctatatcttc ttttcctata agatagttta agatatttaa ctgaacctct
      901 atctttctag tattttccaa agctttattc cagttaacat aataatccca gctagctata
      961 gtacctttta aattttcaag taattcctta aatatttcat ccttactctt atttttatga
     1021 atattcttaa ccaattccat ttgattcccc acctcttcat aattagtaat tagcaattca
     1081 gttataaaac ctcttccatt ccccttagaa tttatgaatc ttttagcatg tactcttttt
     1141 atattaaaat ctgaatacaa atcatcaaag aaagtatctt cttcatctaa aacttttgga
     1201 tcagaattac ttaacatcaa ttttgtatct attttatcta gaactttata aaatttactt
     1261 aactcaattt gactatcatc attaaaatta cctttgttat aggaattaaa tcctcctata
     1321 gttactggtc tatatggtgg atctagatac acaaaacaat ttttatctat tatattttct
     1381 agatttaaat aattcttatt taatattttt acttttagct ctcctcgaga atctctttta
     1441 tttaaaagca tggataattt ttcaagttca tttttatcaa aaaaactagg atttttatat
     1501 cttccaaaag gtacattaaa ttcacctcga gaattttctc tatataatcc attaaaacat
     1561 gttttattta aaaatataaa atatgaagct tgctttatac aatcttcatt ttctatattt
     1621 ttttcatttg taagtatatt aaatctatct ctaatatcat agtacatctt ttctctatct
     1681 gaagaacctt cttcgtaaga tatgtattgt tcttttaaat tagttagttc tgttattaac
     1741 tcttccaaat catctctgac tttaatatat aaattaatta acttactatt tatatcatta
     1801 attacatact tgttgaaaat gtcctcttct aacaaatata gaaatagtgc acctgcacct
     1861 acaaaaggtt ctatataagt catggaacta aaatcacaac ctgtaggcat ttgttttctt
     1921 agaatatcaa gtagctgtgt tttacctcca gcccatttta aaattggttt catgcaacac
     1981 ctctctcagt tatattgtta tataattata taaaataaga ttctaaaata caagtactca
     2041 tcagaacact atatttatat caaatctttc caaaatctaa gtattatttt atcctaacac
     2101 ttttcaaaac ctctaaataa tatacaatat tctctaatat ttacttacta aaaatttaaa
     2161 tctatcttta agtataacct aaaacagatt ataagttacg tagtttttaa tataaaatat
     2221 acttctacaa gtaaagcttt aaatatcaat tacttatttc ataattaatg actatgtacc
     2281 ctcaaaaaaa cctcattttc tatggttggg gtacggttgg tggatttcat caccaaaata
     2341 aaaaatacta gtcaataaag actagtattt ccaagtattt catcattctt ttttcaaaat
     2401 tggtggagac gaggggagtc gaacccctgt ccgcaaatct tttccttgct gcatctacat
     2461 gcttagccaa cttcactaat atctcacctc acatagcgcc aagtggcagg ctctatgctt
     2521 agccagtccc aattaatcta cttcaggtcg agacacccct aaagtagttc cctgcataat
     2581 gacgtccagt cttagtcggc aggaggacta agagggacga gcagcaacta ggctgctatc
     2641 gcgaaattat cgtctgcgtt tattttaaag ttccccagtt tttacgtgac ccagagtaac
     2701 acgacatgca gcctcaagtt ccaaacccac gtcgaaacct ttacgccccc ttgagtagtt
     2761 tatagtccag tagacttttc gcaaacttta tcaccatttt tattaaattt ttaatgtata
     2821 tataatataa tacttttaag aataaaaatc aatgttatgt tctagataat aaagaatttt
     2881 attctaaaaa atacagtttt atctagttta atacttacct gtcatttctc tttctatacg
     2941 tctttgtgca tctttcttag ccatatcttg acgtttatca tatagttttt taccttttgc
     3001 taaggcaatc tctactttaa ctttaccgtt ttttagatac aaactaagtg gtataagtga
     3061 ataccccttt atagtagttg ctccaataag ctttcttatt tcatgcttat gaagtaatag
     3121 ttttctaact cttaaagaat caacattgaa tatatttcct tgctcatatg gacttatatg
     3181 aacttgtttt aaaaatactt cgctattatc tacagatgca aatccatcac ttaggttaac
     3241 tttgccttgt cttattgatt ttacttctgt tccttttaat tcaattccac attcataagt
     3301 ttcttctata aaatattcat gtcttgctct tttgtttgtt gctaatattt ttttaccagc
     3361 catataaatc tcaccttctt tgctagaaat ttattccata aatattagaa atttactaaa
     3421 tatttaatgt tgccaaatta tttgactaac ttgtatgata acatatttag gcattttagt
     3481 caatatttca ctgatatatt aagttagtta ggctatatat aattaagtaa tataaatgta
     3541 tgaaaatagc taaaaaacta cttcgaatat acttaatact aaactaactc taaccagtaa
     3601 tactaattta taaatttcaa aacaaaaata ttacatatta ta
//
LOCUS       DAYAOX010000285         3630 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.288, whole genome shotgun sequence.
ACCESSION   DAYAOX010000285 DAYAOX010000000
VERSION     DAYAOX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.288"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            96..920
                     /locus_tag="ACGI57_003465"
     CDS             96..920
                     /locus_tag="ACGI57_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9718511.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GSNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            1049..2314
                     /gene="gluD"
                     /locus_tag="ACGI57_003466"
     CDS             1049..2314
                     /gene="gluD"
                     /locus_tag="ACGI57_003466"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HFG9718512.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            2527..3117
                     /locus_tag="ACGI57_003467"
     CDS             2527..3117
                     /locus_tag="ACGI57_003467"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9718513.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            3448..>3630
                     /locus_tag="ACGI57_003468"
     CDS             3448..>3630
                     /locus_tag="ACGI57_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9718514.1"
                     /translation="MSNFILNGIFRTVIVSSLSILMILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTI"
BASE COUNT         1466 a          340 c          646 g         1178 t
ORIGIN      
        1 taatattaaa ctgttaatga tataataaat ttaatctata aagtaaataa aattttgtaa
       61 taaatagtta aagctatttt ataaggagat atatgatgga agtatatgct ttggttgggt
      121 caagtggcac agggaagagc tttaaggctt tagagttcgc atatgaaaat gatatagaat
      181 atataattga tgatgggatt ttaatataca aaaacaaggt tttagctggg atatcggcaa
      241 aacaagcaaa tactactata gaagcagtta aaagggctat tttttataat ctggagcata
      301 gacaagaagt tagagaagca attaaaaaag aaaatataag aaaaattctt attattggga
      361 catcaaaaaa aatggttaat caaattgtag agagattgtc aattggaagg gtttgcaagt
      421 ttataaatat acaagatata agtactaaat atgaaataga aatagcaaaa caagctagaa
      481 aagaaggaaa tcatgtaatt cctgttccag cagttgagat aaaaagtatg gcaagtggtc
      541 ttagtataaa ttctttgaag agacttttta gaaaaggaag taataggaat atggtagttt
      601 tagaaaaaac aattataaga ccaactttta gttatatagg aaagttttat attagtgctg
      661 atgtaattaa acagattatt gaatatgaag tatacaactt tggaagcatt gatagaataa
      721 ataaaatcaa tatagagaat tgcaataatt ttatgaatat ttttgtaagt ataaatatca
      781 atgatttaga aactataaaa aaaattgaag acatacaaca gacaataaaa aagtctattg
      841 agaaaatgac tttagttaat gtacaaaaaa tagatattaa cattcataaa attaaaaaaa
      901 taaacttcct ccaatgttaa aaaataaact gagaaaatga tatactaatt tgcaataaag
      961 tgtgctataa ttaaactgta aatttaggaa atggtttttt ctaaatggag tatacataat
     1021 tataacgtat ttataagggg gctttcaaat gtcaggaaaa gatgtaaatg tcttcgagat
     1081 ggcgcaatct caagtaaaaa atgcatgtga taaattaggt atggaaccag cagtttatga
     1141 attattaaaa gaacctatga gagttataga ggtttcaatt ccagtaaaaa tggatgatgg
     1201 ttctataaaa acttttaaag gatttagatc acaacataat gatgcagtag ggccaacaaa
     1261 aggtggaata agattccatc aaaatgtttc aagagacgaa gtaaaagctt tatctatatg
     1321 gatgactttc aagtgttctg taacaggtat accatatggt ggaggtaaag gtggaataat
     1381 agtagatcca tcaactttat ctcaaggtga attagaaaga ttaagtagag gatacataga
     1441 tggaatatat aaattaatag gtgagaaagt tgacgttcct gcaccagatg taaatactaa
     1501 tggtcaaata atgtcttgga tggttgatga gtacaataaa ttaactggac aaagttctat
     1561 aggtgttata actggtaagc cagttgaatt tggtggttct ttaggaagaa cagctgctac
     1621 tggatttggt gttgctgtta ctgcaagaga agctgctgct aaattaggaa tagatatgaa
     1681 aaaagctaaa atagctgttc aaggtatagg aaacgttggt tcttatacag ttcttaactg
     1741 tgaaaaactt ggtggtactg ttgtagctat ggctgaatgg tgtaaatcag aaggttctta
     1801 tgctatatac aatgaaaatg gtttagatgg tcaagctatg ttagattata tgaaagaaca
     1861 tggtaactta ttaaacttcc caggagctaa gagaatatct ttagaagagt tctgggcttc
     1921 agatgttgat atagtaatac cagctgcatt agaaaactct ataactaaag aagttgctga
     1981 atctataaaa gctaaattag tttgtgaggc tgctaatgga ccaactactc cagaggctga
     2041 tgaagtattt gctgaaagag gaatagttct tactccagat atattaacta atgctggtgg
     2101 agttacagtt tcttacttcg agtgggtaca aaacttatat ggatactact ggtcagaaga
     2161 agaagtagag caaaaagaag aaatagctat ggttaaagca tttgaatcta tttggaaaat
     2221 caaagaagag tacaatgtta caatgagaga agctgcttac atgcattcaa ttaagaaagt
     2281 tgctgaagct atgaaattaa gaggatggta ctaagattta ctcttaataa ataataggat
     2341 ataattattt aagatataaa ggggctgtac atagtacagc ccttaaatta tgtggcttac
     2401 aatgaagtaa gctatttttg ttttaactta taaaaaactt gataaattat gtaatttaga
     2461 atgtattttt taccaccaca tgattacaaa ttagaagagt tagattagat ttttagacat
     2521 gaacttatgc attttaaaag caaagacctt aatttaaagt atctagtatt atttttaaaa
     2581 atagtatatt ggtttaatcc atttatatat ataatggaaa aatatatgga tttggattgt
     2641 gaactttatt gtgatgagag agttttaaaa aattgtactg tacaacaaaa gcaagaatat
     2701 gctctaacta ttgtaaattc aatgaaaaga agtacaaatt tttcaaataa atttgtagct
     2761 ggattaaata aacaatctga tattagaaaa agagtaataa acatgttcag tgaaaaacat
     2821 aaaaatggta tcttaagggc tttgattata agcttgtttt cttctattac attttttaaa
     2881 ttcgattcaa ctagtattat tgaagagaat gatttcttag agaggtcaag atatacgttt
     2941 gaaggaaggc aagaagacat gtttgtaaca atagatttta tatatgcaga tgtacctata
     3001 aaacttagaa aagagcatga agaaaactgt agagtcaatg gaatagtacc aaaagattca
     3061 gattcaatag aaattattcc tagattttat aaagattgta tggatttaaa aaaataagtt
     3121 agacttaata ttgatgttga tattatgaat tttttatatt taagcaattg aaaattttat
     3181 tatagattca attttatatg tgtttaagta tgaaatttat agatagttga aataattaaa
     3241 tatgattgat gcataggaat cattactaag ctataattaa ttataaggga gtttaaataa
     3301 attatgagtt acacaattat aaaatgataa aaaatagtta tctgttacaa aaaatgtatc
     3361 tttttaatta ataaagataa tatgaaaact taaaaggcaa ataaacttaa acataaaata
     3421 ctcttaaata agataggaga aaaatgtatg tctaacttta ttctaaatgg gatttttaga
     3481 acagttatag taagtagtct aagcattctt atgatattga ttttcaagaa aaatgtattt
     3541 aaaagattta gcaaaaaatt taattattat atatggatga tagttgttat taaattattt
     3601 ttaccattta catattatac ttttacaata
//
LOCUS       DAYAOX010000286         3619 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.289, whole genome shotgun sequence.
ACCESSION   DAYAOX010000286 DAYAOX010000000
VERSION     DAYAOX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.289"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            112..1608
                     /locus_tag="ACGI57_003469"
     CDS             112..1608
                     /locus_tag="ACGI57_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9718515.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            1742..2668
                     /locus_tag="ACGI57_003470"
     CDS             1742..2668
                     /locus_tag="ACGI57_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9718516.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            2786..2977
                     /locus_tag="ACGI57_003471"
     CDS             2786..2977
                     /locus_tag="ACGI57_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718517.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            3200..3550
                     /locus_tag="ACGI57_003472"
     CDS             3200..3550
                     /locus_tag="ACGI57_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HFG9718518.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         1451 a          351 c          681 g         1136 t
ORIGIN      
        1 ttaacaaagt atatgcctat gttataataa tttatagaaa taaataaaaa atacaatata
       61 cataatagta gaaatatagc aaaaaggtgt gatttatagg gaggattact tatgtttgaa
      121 tttgaaacgc aactaaaaag gttaaaacat gaagttttaa cagcagtagc aagactatct
      181 attgaagaaa atttaacaaa agaaaatata gaaaaaattc catatgaaat aattaaagga
      241 gaaaaggcga gatatagatg ttgtgtttat aaagagagag aagtagttct tgaaagggcg
      301 caacttgcat taagtttaac tccaaatagt aaatatggag atataaatcc agattcagtt
      361 agtttggaca cagatgagca aattatatat atagttgaag cagcatgtga tagatgtcca
      421 ataaatgaat ttacagttac agagctttgt agagggtgtt tggctcatag atgtaaggaa
      481 gcatgtaaat ttggggcaat ttcttatata aatgggagag catatataaa tcatgattta
      541 tgtaaggctt gtggaatgtg caaaagttca tgtcaatatg atgctatatc agaagttgta
      601 agaccttgta aaagcgtttg tccaacagga gctttggatt ttaatagaaa cactatgaaa
      661 gcaatgatac atgaagataa ttgtataaat tgtggagcat gtatgtcagc atgtcctttt
      721 ggagcaatat ctgataagag tttgatagca cctgttgcaa aaaaacttgc aaagaaagaa
      781 aatatgtatg caatagtagc tccagctata acaggtcaaa tagatagtaa tattacatat
      841 ggtcagttaa aaaatgctat aaaatcactg ggatttgtag atatgataga agcttcttgt
      901 ggagcagatg cagttacagt tcatgaaagc aatgaactta ttgaaagaat aaaatcagga
      961 gataattaca tgacaaattc atgttgtccg ggatttttta agtatataga gaagatgttc
     1021 ccagatgaaa aagatagaat atcatctaca gtgtctccta tggtagcaac aggaagatta
     1081 gtaaaaaaaa tagattctga tgctaaagtt gtatttatag gtccatgtac agctaaaaag
     1141 agtgaggcaa cagagaaaag tttaaaagat tcagtagatt atgtacttac ttttgaagaa
     1201 ctaatggcat tatttgaagc atttaatata gatttatcaa aatgttcaaa tgaagatata
     1261 aatgatggtt cgatttttgg aaggggattt ggagcatctg gtggacttgc caaatcagta
     1321 cagaactata ttgctgaaaa ggggcttagt tttgattttg aacctataaa agtatctggt
     1381 ccaagacaaa ttaaaaagac aatgacaatg gctaaattgg gaagattaga aggtaatttt
     1441 atagaaggaa tgatgtgtga agaaggttgt attaatggag ccggtaagtt tttatcaggt
     1501 gtaaaagcca aaaatacttt tgatagaaaa aatggacaat ctactaaaaa gagtgtttta
     1561 tctaatgata agattaaaga atttgaagat gtaaatttag aaagataatt gttttggtac
     1621 agtcattttt ttattatgcc acaaatatgc ctaaaatttg ccctttttat atgtgtattt
     1681 tacagagtta aggttgctat actataaata tgtaataaat aaaatttata ggagcttttg
     1741 gatgaggaaa atagtattaa aagatatcct gtttttagtt actacattat ctataatagt
     1801 aatattattg cttgtaacaa atataactac taatagtaag ataaaatcaa caagtgaatc
     1861 ttatttaaaa agtgacaaaa atagtgtgag gcaaaatggt atccagagga gcacaaagag
     1921 tagcacaaaa aatactgcat ttaagactac tatactaaac actggaaagt ctgattgcat
     1981 attggttgag ataggtaaca aagtcataat gattgataca ggagaagata aaaatggaaa
     2041 acaaatagtg gataggttaa aagaaaaagg aataaataca cttgactatt tgattttaac
     2101 tcatttagat aaggaccata taggtggagt agatagtgtg ttatctagtg ttaaagtaaa
     2161 gaatattata caagcaaatt ataagaaaga tagtaagcaa tatgatgaat atattgactc
     2221 acttaaaaaa gctgatatag aaccagtgct actaaaagat aatatgaata ttgtaatcaa
     2281 tggtgcagaa ataaacatac atccggcttc aaaaagcaag tacgagagtt caaatgatta
     2341 ttcaataatc actaatataa gctatggagc acataagttt ttatttgcag gagatgcaga
     2401 ggaagaaaga ttgtcagagt ttataaatgg aaatacttta aaatatgatt ttgttaaaat
     2461 gcctcatcat ggaagatatg acaaattaac tgaaacattt ttagagtcta tttcacctca
     2521 atatgctgtt attacatgtt cagagaaaaa agaaccagag gaaggtgttt taaaaatatt
     2581 ggaaagattg aatataaaaa catttttgac ttctaatgga gaggtagtta tcaatagtga
     2641 tggtaagaca ttgagtgtga atcaataatt tttttaaaaa tatctaaaaa aatttaaaaa
     2701 aatcattagc aaatattacc ccttaaacat agtatatttt aggagctgaa actccacata
     2761 caaaatgact aggaggtaac atattatgtt ggatagattt tttggtgaag gcggatttgg
     2821 tggatgtgct tggtggataa taatattatt cttcttattc ttagcattcc aagattgttg
     2881 ggctgaaatg gacttatgtg catggatacc tttcttaata ttattattaa tactttgtga
     2941 atgtggtgac gtttttgatg gaggatgtgg atgctaaaaa aatcaattta gatgatttaa
     3001 taaagcttat ggtgggtata aataaaatac caaaccaatc acaatctagt gtttgaaggt
     3061 agtcggaaat cccccgctac cttctttttt ttattgttaa aatttatttt tgaattattt
     3121 catttacata taataatttt tacgataaag tctgatataa ttaaaaagaa aataaaattt
     3181 atttatagga gatgtaaaaa tggctatagt ggataagatt ttcaatgaga taaaacttat
     3241 ggttgcaaga cttttattgg gaaaaaaata cagtgaatat gaagaaaaag atttagtgta
     3301 taatacagaa gaagaagtta tacttataac tttaaaaaga ttagtttttc agggaaatgt
     3361 aaacgaagca gaagaaattt tatttgataa agctaagagt gtaaatagtg aaaatatgca
     3421 gtatatagca atagaatttt atactatgct tatggaaaag actgatgaag aattagaagc
     3481 tatgaatttt tctaaacaag aagtttatca aggtatagaa gatattagaa aagtattgaa
     3541 tttagaataa aagatatatg aaaatattga aataaattaa tgctaaggtg agaatattat
     3601 acttatatat aaaaatata
//
LOCUS       DAYAOX010000287         3561 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.290, whole genome shotgun sequence.
ACCESSION   DAYAOX010000287 DAYAOX010000000
VERSION     DAYAOX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.290"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            150..1301
                     /locus_tag="ACGI57_003473"
     CDS             150..1301
                     /locus_tag="ACGI57_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFG9718519.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1347..2522
                     /gene="thiI"
                     /locus_tag="ACGI57_003474"
     CDS             1347..2522
                     /gene="thiI"
                     /locus_tag="ACGI57_003474"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFG9718520.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEESTILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2678..3481
                     /locus_tag="ACGI57_003475"
     CDS             2678..3481
                     /locus_tag="ACGI57_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFG9718521.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
BASE COUNT         1422 a          346 c          715 g         1078 t
ORIGIN      
        1 ttgtaataaa gtgaaaaaac tagcttttca tgaaataata ttttataatt taagattgtc
       61 tttttaataa agattaacaa aaaaaatact ataattagtg gtataatagt tagagttgaa
      121 gattttaaat aaaggtaggt gtaattaagt tggaaatata cttagacaat agcgcaacta
      181 caaaacctta tcaagaagtt atagataaaa tggtttatgc acttaatacg gaatatggaa
      241 atccttcttc tgtccataga aaaggtgtag aagttgaaaa agctataaaa gaagtaagac
      301 aagatatagc taaatctcta ggagcgaaag aaaaggagat ttattttaca tcaggtggta
      361 cagagtgtaa taatactata ataagaggta ttactagttt aaataaaaaa agaaaaaatc
      421 acataatatc tactaacata gaacatcctt ctgttttaaa tacattaaaa gatttagaag
      481 aagatggttt tgaagtaact tatttagagg tcggaaaaga tgggaaaata aatatagagg
      541 atttaaaaaa tgcgataaag tcaactacat gcttagtaag catgatgcat gttaacaatg
      601 aaataggaac tatacagcca ataggtgaag ttggaaagta tttgaagggt ttaaaagaaa
      661 aaatttattt ccatgttgat gctattcagt catatggaaa aattaacttt agaccatcta
      721 aatacaatat agattttatg agtgtaagtg cacataaatt tcatggtcca aagggaattg
      781 gatttatgta tataaaagaa aataatcgcc ttaagcctat gttaacaggt ggtggtcagg
      841 aaattggaat taggtcggga acagaaaatg tacctggaat atatggtctt ggagaagctg
      901 ttagaatact aaacaaagat ttagatgctg ttatttcgaa agtagataac ttaaaaaata
      961 tattaaaaaa tgaaataatt gataatatag aagatataaa aatcaattct ccagaagacg
     1021 gagtatgtca tatattaaat gtatcattta gagggacaaa aggagaagtt ttacttcatt
     1081 acttagagca aaaaggtatt tatgtatcaa ctggctctgc atgttcttct aaaaagaaag
     1141 gtagttacgt attaaatgct ataggtctta caaatgaaga aataaatgga acaataagat
     1201 ttagtttatc tgatatgaat acagaagaag aaatgttaga agctgtgaaa gttttgaaag
     1261 aatcaatttg tgacttaagg tcaataatga aaagaaaata aagaatactg tataaatagt
     1321 gataataaat tagtttaaga ggtgaaatgg tgtataatat attaatagta aagtatggag
     1381 aaattggagt aaaaggaaag aatagatata tatttgagaa taggcttata agaaatataa
     1441 gaaatatgtt aaaaccaatt ggaagattta atgtatataa agagtatgga agaatatatg
     1501 ttgatttaga agactatgat tatgaagaag taattgaaga agttagaaaa gtatttggga
     1561 tagtgggtgt gtgccctgga gtaagagcta aaaaagatta tgatacatta aaagaaatag
     1621 ctttaaaaat gttagaagaa aaaatagagg ctggatataa aacttttaag gtagagtcaa
     1681 gaagaggaga taagtctttc ggattgactt ctcaagaaat gagtatggat ataggtggat
     1741 acttattatc aaaggtagga gataggatta atgttgatgt aagaaatcca gaagttaaaa
     1801 taaagtgcga atacagagaa tttcatacta tggtctatag tgatactata cctggatatg
     1861 gtggattgcc acttggaacg aatggtagag ctatgtctct tttatcagga ggtatagata
     1921 gcccagtagc tacatggatg gttgcaaaaa gaggtatgga agtagaggca gttcactttc
     1981 atagttatcc atttacaagt gaaaggtcac aggaaaaagt taaggattta gctaaaatac
     2041 tggcaaaata ctgtggtaga gtaaggcttc ataaagttaa tatcttagaa atacaaaaag
     2101 caattggcga gaattgtaat gaagaagaat ctactattct ttcaagaaga tttatgatga
     2161 ggatagcgca gagactttct gaaaaaagac attgtgatgc tttaattaca ggtgaaagta
     2221 ttgggcaggt tgcatctcaa actatacaag gacttacttg tactaatgcc gttgtagact
     2281 taccagtgtt tagaccactt atagcaatgg ataaatcaga tatagttgat atagccaaaa
     2341 aaatagggac ttttgaaaca tctatagtgc ctgaagaaga ttgttgtagt gtattttctc
     2401 ctagaaagcc cgtcactaaa cctagattag agaaaataga aaaatctgaa acagcacttg
     2461 acattgaaaa attagtacag gatgctattg acaaaataga ggtagaggat attgagtttt
     2521 agcatacaag ttatttaatt actaaattga atattgagta tattagtagt tctgtaagta
     2581 tattagtagt tctgttgaaa ggatttatgt agacaattta ttaatatgta gtatttaaaa
     2641 ataagtacta taattttatt taatgggaat gattattatg aataaaaagg gttttacact
     2701 aattgaattg ttagtagtta tatctataat aggcatttta gttatagtag ctgttccagc
     2761 attgtttaga aatatagaaa aaagtaaagc agttacatgt ctttctaata gagaaaatat
     2821 aaagactcaa attgttattg caatggctga ggaatcaagt aaaggcaaga atgaagtcat
     2881 gaaagaggta ttagaaaaca aagatggtaa gtactttgaa acagaaccaa agtgtaagtc
     2941 aggtggaata tattcagcaa cgtttgatga tggttatgat ggaataactg gaatagaaag
     3001 cattgcaaaa gtgtatgtta cttgtacaaa acatccagat ggtgttgaaa tggctaggga
     3061 tatacatcaa agtatgaaag atttgattgc atcatttgca caagaccctt ctataatacc
     3121 aggagcctca aagggtaatg atgattttag aaaatattta ttagacaata aatataaaaa
     3181 tgggtggcct acaattccag atgaatttaa ggcaaaatat ggattaagta aggatacact
     3241 atatatacaa ccatatgcat ataatcctac taaatctgat gctactgtag ttgtatttgc
     3301 aaataataag actgggggta attggtatac ttccctagtt tacgattatg atgaaggtag
     3361 atggtataaa ggtaaaaatg gtatttctgt tgcaggtagg tcatgggatg ttgacacaga
     3421 tagtgttaag tctgtaaaaa cagagattca ttctaaagag ggatggggtc ctttaaatta
     3481 atatatcatg ttttattaat ctggtattga ttaaactata gaaaagagca gtttctatag
     3541 atgacgaata ccagattttc a
//
LOCUS       DAYAOX010000288         3501 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.291, whole genome shotgun sequence.
ACCESSION   DAYAOX010000288 DAYAOX010000000
VERSION     DAYAOX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.291"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            287..832
                     /locus_tag="ACGI57_003476"
     CDS             287..832
                     /locus_tag="ACGI57_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFG9718522.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            845..1858
                     /locus_tag="ACGI57_003477"
     CDS             845..1858
                     /locus_tag="ACGI57_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFG9718523.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            2080..>3501
                     /locus_tag="ACGI57_003478"
     CDS             2080..>3501
                     /locus_tag="ACGI57_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9718524.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTI"
BASE COUNT         1381 a          341 c          638 g         1141 t
ORIGIN      
        1 ttaataataa aagaaataaa aataagttct taaatttcta agaagaattt agaatatagt
       61 cgaaataaga attgtatatt tctgaaaatt ctaattagta caattttttc ctttataaaa
      121 ttgaaaaaag atgtatttat ttatcaaaaa tattatatta aaatgatatt aataaaaaaa
      181 catttacaat attagcatat agtacagaaa aaagatgtaa tttgtaaaat ttatggtaag
      241 cagtaaaatt aaaaatttaa agatattata gggaggttat aattttatgg aaaacgttat
      301 tacaggtcta agtaaaggtg cagagtggtt tatcggtttg tttcagaaag ggggagaaca
      361 atttataggt cttgtttcag gtactcttcc aacgctaatt gtattaatgg tagctattaa
      421 ctctttgatt aagataattg gtgaggaaag agttaataat tgggcttcta agctaggaaa
      481 gaactctttt acaagatata ttctacttcc attagtatca gtatttttct tgggaaatcc
      541 tatgtgctat acatttggta gatttttaaa agaagagcac aaagcaggat tttatgatgc
      601 agcagtttca tttgtacatc caattacagg tctttttcca catgcaaatg caggagaact
      661 ttttgtatgg ttgggaatat ctgcaggttt gactacttta ggtaaagaaa ctactacatt
      721 ggcattgtgg tattttatag ttggtctaat tgtaattttt ataagaggta tagtaacaga
      781 aaaaatgtat gcttttctaa caagaaaaaa tagagcaaca aataaggctt aggaggtaga
      841 taaaatggag gagaataaaa tattaaaaat agaaaaggga acttcaggct ggggaggtcc
      901 tttgtatata aaaaaagaag ggaatagaaa taagatacta tcaatgactg ctggcggtat
      961 ccatgaagta acattaaaaa taaaagaact actgggttgt gaaattgtag atggatttaa
     1021 aactggagtt tctgatgaag aagtagcagt agttattatt gattgtggtg gaactgctag
     1081 atgtggagtt tatccaaaga aaaaaatacc tacaataaat gtaaatccag ttggaaaaac
     1141 aggtccttta gcaaaattta taacagaaga atattatgta tcagatgtta atcctaactg
     1201 tataagtgtt gtagatgggg aagatatgcc acaaaaatct caagaaaata aaagcgaaaa
     1261 taagagttct attagaaagc ctgataatta tgatgaggtt aagtcaaaag cacaaggtga
     1321 atatgctaaa aagaacataa tactttctat tggtcaaggg gcagggcaag tagtatcaaa
     1381 attttatgat gcaggtcgag atactatcca aatggtaatg aataacgtaa taccatttat
     1441 ggcatttgta agtatgctta tgggaattat tttagcatct ggactgggag actggattgc
     1501 aagagttata tctccacttg caggtaatat tggtggatta ttgattatat cagtaatttg
     1561 cacacttcca ttcttatcac caatactagg acctggtgca gttatagctc aagtagtagg
     1621 gactcttgta ggtacacaaa ttggtcttgg tgcaatacca gcatatttag cacttccagc
     1681 tttatttgca ataaatgggc aagctggatg tgattttgta ccagtaggat taagtttggg
     1741 agaagcagaa ccagaaactg tagagtatgg tgttccagct ttattttact ctcgtttaat
     1801 aactggtcca atagctgtta ttattgcata tggagttgca gtatttgcat tgagataata
     1861 tttaatgtca atataaggct tggaattaat taataaaatt gtaaatataa atttaagcta
     1921 gtcaagattt tttgttaatg aaagactagc ttttatacta ttaattagct gtaattatcg
     1981 agttagatta atactaaatt ttcctttatt aaagtgaaat tttatgtatt aatttggttt
     2041 caaatttgta ctagaatgaa atcaaggagg agatacaaaa tgagtgtaac tgagaaaatt
     2101 ccttctattg accgcaagat atttaagata ttgattatgt gtagtaaaaa agagtttgtg
     2161 agtattaact caatagcaaa tgaattgaat gtcacaacta gaagtgtaag gacgtatata
     2221 aaacaattga ataaagattt aggtaatgat atagcagtaa tcaaatatat aaagggtcaa
     2281 ggctataaac tagaaataaa agatgagcag atactcaata agattattga tatgaataga
     2341 aaaaatgtat tttcattaaa ttcaaaagaa gatagagtag agtttatact aaattacttg
     2401 atagaactag atggatttat tacacttgat agtttagctg atgagatgtg tgtaggaaga
     2461 actactttag taaatgattt ccagtatgta gaaaaagttt tggcttcata taatttaaac
     2521 ttgattaaaa aacagaatac aggtatgaaa ttaaatggaa atgaacttga tattagactg
     2581 tttatattaa atcagttgta taaaaactca aggaaagatt tcagtaattc aaagtatttt
     2641 aaaggaatca aaaaagaaga aatcataaat ttagaagaaa aactattgac tttatttaaa
     2701 aaaaataatt tttatgttac agatgaaatg ttaagagaag ttattaacta catagtagtt
     2761 ttagtctata gagtaaaaga agaaaagaaa gtaaaagatt atgatgtaaa gtttgattta
     2821 cttaaatcat atgatgaata ctttattgct agggaaataa aacatataat atcaggaatg
     2881 tttgaatgta ctttaaatga tgaagaaatt atttatttaa ctataccgtt agttagtgga
     2941 aatgcaccag catcagaatg tgctttaaat agtagtagaa ttagtaaaaa cattgatgag
     3001 ttgatggaaa atatatttaa tcaaatatat gtcgatatgg gtatttttat aaatgaggat
     3061 gaattaagag taggtctagg atatcatctt agttttactt taaacaggct attatttaat
     3121 ataaagttaa aaaatgtttt gttagaagag attaagcaaa attatatact tccatttaaa
     3181 ttagcacaaa tagcaggaaa agtaatagag aaaaagtata acttagaagt ttcagaagat
     3241 gagattggat atatagcaat ccatttcagt ggatatttag aaagaaatag cagtagattc
     3301 tattcaataa aaaaaatagc aattatatgt agtacaggtc taggaacagc aaaactattg
     3361 aaaataagag tagaaaaact gataggaaat aatcctaaaa ttgatactct ttcaagtttt
     3421 aatttgagaa atattaactt agatgaatac gatatagttt ttacaactat tgatttagac
     3481 acatcaaata taaatacaat a
//
LOCUS       DAYAOX010000289         3498 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.292, whole genome shotgun sequence.
ACCESSION   DAYAOX010000289 DAYAOX010000000
VERSION     DAYAOX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.292"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            100..2601
                     /locus_tag="ACGI57_003479"
     CDS             100..2601
                     /locus_tag="ACGI57_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFG9718525.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2761..3429
                     /locus_tag="ACGI57_003480"
     CDS             2761..3429
                     /locus_tag="ACGI57_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9718526.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
BASE COUNT         1434 a          368 c          674 g         1022 t
ORIGIN      
        1 aaatatagat ttctaaataa gtaatttatc aatattacat atcaaaataa aacgttacaa
       61 attagctgaa aatatattga aagaaaggaa gattttatta tgagttcaaa taaagtggta
      121 gaaagttata aaattacagg tatgacatgt gcagcttgtg caaaggcagt agagagagta
      181 actaagaaga tggatggagt atatgaccaa agtgtaaata tagctactga aaaacttaaa
      241 atagaatatg ataattctaa ggttaatttt gatgatataa agcaggttgt agaaaaagca
      301 ggatatggaa ttataaagga agaaagtaat aaaaaaattg atatgaaaat tgatggaatg
      361 acatgtgcag cttgtgcaaa agctgtagag agagtggtaa aaaaattgga tggagtagaa
      421 agtattagtg tgaatattgc aacagataaa gctaatatag actatgaccc atcaaaagta
      481 aaattatctc aaataaaagc agctattgaa aaagctggat ataaaccaat agaagaggta
      541 aaaaataagg ttgatgtgga tgaagataaa ttgagaaaag aaagagaaat gaagtcttta
      601 tttgttaaat ttatagttgc aatagtattt gcagtacctc tgttttatat agcaatggga
      661 cctatgataa taaagcctat agggccatgg cctttgccag aaattataaa tcctatgaca
      721 aatactttta attatgcatt aattcagtta attttagtaa taccagtaat gatagctggt
      781 tataaatttt atataaatgg gtttaaatca cttttttctt taagccctaa tatggattct
      841 ttagttgcaa taggaacatt agccgcattt ttatatagtc tatatacgac actacaaata
      901 gccaatggac aaattcaagg tatgcaccat catcagttgt attatgaaag tgcaggaata
      961 ataatagcac ttatcttact tggtaaatat ttagaatcaa aatcaaaagg aaaaacatca
     1021 gaagcaatta aaaagcttat ggggctacaa cctaaaactg caatagtgtt agttgatggt
     1081 aaagaagttg aaactcctat agaagaagta gagataggag atatactatt agtaaaacca
     1141 ggaactaaaa tacctgtgga tggagttgta atagaaggat atacatcagt tgatgaatca
     1201 atgcttacag gtgaaagtat accagtagaa aagaatgttg gaagtaaggt gacaggagct
     1261 agtattaata aaaatggtgt tattaagttt aaggctgaaa aaattggtgg agatactgct
     1321 cttgcccaaa taataaagtt agttgaagat gcacaaggta caaaagcacc tattgctaaa
     1381 cttgccgata ctgtatctgg atattttgta ccaatagtaa ttgcaatagc tgtagttgca
     1441 tcattattgt ggtttttaat aggaggaaaa gatatagtat ttgtacttac tatatttata
     1501 tcagtacttg taattgcttg tccttgtgca ttaggattag caacaccaac tgctataatg
     1561 gttgggactg gaaagggcgc agaaaatgga atcttgataa aaggtggaga ggcattagaa
     1621 tctgcacata aagtaaatac agtgatattt gataaaacag gtactataac agaaggaaag
     1681 ccaaaagtaa cagatatagt attaaataat aatgtaaaag aagaatatct tatcaagatt
     1741 gcttcaagtg cagaaaaagg ttcagaacat cctcttggag aagcaatagt taagtatgga
     1801 gaagagaaga atataaaatt tgaaaaagtt gataatttta aagccatacc aggtgctggt
     1861 atacaagtta ctattaatga tgaaagtata ttacttggaa atagaaagct tatgaatgac
     1921 aataatataa agcttgggga tttagaagaa aaatcaaata tattggcaag ccaaggtaaa
     1981 acacctatgt atatagctgt agatggaaat ttatcaggta taatagcagt tgcagatgta
     2041 gtcaaagaaa gtagtaaaaa agcaatagaa atacttcatg atatgggaat aaaagttgca
     2101 atggtaactg gagataatgc gaaaactgca aatgctatag caaatcaagt cggtatagat
     2161 atggttttgg cagaggtttt accagaagat aagtcaaaag aagttgaaaa actacaaaat
     2221 caaggaaagt ttgttgcaat ggtaggggat ggaataaatg atgccccagc tcttgcaaaa
     2281 gcagatatag ggatagcaat aggaagtgga acggatgtag ccattgaatc tgcggatata
     2341 gtacttatga agagtgattt aattgatgtt ccaacagcaa ttaaacttag tcatgaaact
     2401 ataaaaaata taaaacaaaa cctattttgg gcatttggat ataatacaat tggtatacct
     2461 gtagcagcag gaatattata tgtatttgga ggacctttat taaacccaat gattgcagca
     2521 gcagcaatga gcttaagttc agtttcagtc gtatcaaatg cacttagact taaaaacttc
     2581 aaggcatata agagagatta gattaataaa ttaaataaaa tgtatccaaa agagctatct
     2641 ttaaattaaa aggattaatt tacaaattaa gatagctctt ttttcatgta aaattcacaa
     2701 caataagata aaatataagt aatatacgaa attttaaata aaatagaaag gattgataga
     2761 atgaattcgt caatacttgt aattgaagat gattcaaata tacaagagtt aatatcagaa
     2821 tttttaagtg cagaaggtta tcaagttgat acagcaaatg atgggttgga aggtatacaa
     2881 aagtttaagc aaggaagtta tgatttagta atattagata taatgatgcc aaacttagat
     2941 ggttatggag tttgtaaaat gataagaaag tcatcaagcg ttccaattat atttttaact
     3001 gctttaaatg atgaaggaga ccaacttaaa gggtttgact tagaatgtga tgattatata
     3061 acaaaaccat tttcatttaa tcttcttata aaaagagtag aagcaatttt aaggcgaagt
     3121 aacaagacta taaatgacaa atttatagtt tttgaaaaat taaaattaga tttaaacaca
     3181 tatattgcag agatagatgg agaacctata gaacttacac taaaagagtt taatatatta
     3241 aaagctttga tagaaaagta tccacaagtt ataactaggg aaggtctttt ggatagtata
     3301 tggggttatg actattatgg agatactagg attgttgatg cacatataaa aaatataaga
     3361 aaaaaaatat cattaccata tattaaaact gtaaaaggaa taggatatac acttgagaag
     3421 gatatttgat aagtgggaaa agttaagtat aaagtataag ttattttcaa taacgacatc
     3481 attattaata gcattagc
//
LOCUS       DAYAOX010000290         3483 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.293, whole genome shotgun sequence.
ACCESSION   DAYAOX010000290 DAYAOX010000000
VERSION     DAYAOX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.293"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(46..618)
                     /locus_tag="ACGI57_003481"
     CDS             complement(46..618)
                     /locus_tag="ACGI57_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFG9718527.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(647..913)
                     /locus_tag="ACGI57_003482"
     CDS             complement(647..913)
                     /locus_tag="ACGI57_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HFG9718528.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(913..1122)
                     /locus_tag="ACGI57_003483"
     CDS             complement(913..1122)
                     /locus_tag="ACGI57_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HFG9718529.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            1391..2251
                     /locus_tag="ACGI57_003484"
     CDS             1391..2251
                     /locus_tag="ACGI57_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFG9718530.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2299..3327
                     /locus_tag="ACGI57_003485"
     CDS             2299..3327
                     /locus_tag="ACGI57_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFG9718531.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVVYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
BASE COUNT         1357 a          389 c          559 g         1178 t
ORIGIN      
        1 taaattataa attatcaaat aataatagtt atacaaataa aaattttaga acattttttg
       61 agattcttct aactcgtaag catccattaa agcttcacca aatctttgat agtgaattat
      121 ttctctttga cccaagaatt ttaatacatc tataacatca acgtcatctg ttaggtttat
      181 aagttgataa taagttgcaa gtgctttttg ttcagctgcc atatcctcat gtaaatcggt
      241 tacgggattt gacataactg ctataggtct tacatcaaat ggaacaccat ttgaatctgt
      301 tggataaatt ccatatccat tttgagcata gtttgaacca agacctgcag cttttaactc
      361 ttctgggctt gcatcagaag ttaactgata aaccatagta catataagct caacgtgagc
      421 tagttcttct gtaccaatat ctgttaaaag tgcacgcata ttaccagtag gcattgtata
      481 tctttggctt aaatatctta aagatgcagc aagttctcca tttggcccac caaattgagt
      541 tataagaaat ttagccattc tagggtcaca agtttttata ttaactgggt gttgtatagt
      601 tttctgatat atccacatga attttcaatc ctccaatact tgatgtctaa taattaaatt
      661 ctctgtccca aggccaaggt tggtcagccc attgccaagg gcatctactt ggagcatacc
      721 caaagttagt taaaggaccg tatttatttt cgtaagcata tctagcttgc gcaaattgat
      781 tacataatga attataagtg ttgatagcat ttttatcatc agggtgattg tctaaatata
      841 agttcatatc aacacatgca aaagatgttt cctgaacttt tctcataagt tcagctctag
      901 atgagtcttt cattatcttc tacctcctct atattttttt ggcttagcat ataattcttt
      961 gttgtacatt tcagactcta gtaagtttag ttctggaaat atagttccaa attttaatgc
     1021 atcagaaagg cagtacattt ctgtgaaaat ttgattatta atgtatggtc tagccaaaga
     1081 tggaccacaa ttttttttga tatctctagt cttattttcc aagatgaaaa cctccttgtt
     1141 gtaaatagat aggtaactct atctagtgta taatattcaa tcatttaaaa atcgttacta
     1201 ataataaaat attaaatgag aaaaataaaa aagataataa aagaaagagg ttatttatgt
     1261 tattataagt ttatgtgtta ttgtaaacta taaattattt agtaaaatta aagtaaaaat
     1321 tatagtattt tgtggatgaa ggtagacttt tttgtaaaat attattaaca taaatatacg
     1381 aggtgattag atgagtgaac tatcaggaaa aggctgtgaa attatagttc cttttgagga
     1441 aagacttcca gtaagagata tagaaaaaag tataataaaa aaatatagaa aaaatttatg
     1501 gtctaagttt atgaaggcaa taagagatta taaattggtt gaagaaggtg ataagatagc
     1561 tgtagcgatt tctggtggaa aagacagtat tcttatggct aaaatgtttc aagagttaaa
     1621 aaaacatgga caagttaatt ttgatgttga atttattgct atggaccctg gatatcatgc
     1681 aaatataaga caattactta tagataattg tgagtatcta aatataccaa tacatttatt
     1741 tgattcaagg atatttgaga tagcagatga aatagcaaaa gattatccat gctatatgtg
     1801 tgcaagaatg aggagaggag cattatattc aaaggcagaa gaactagggt gcaacaaact
     1861 tgcattaggt catcattatg acgatgtaat agagacaaca atgcttaact tattgtgtgc
     1921 tggaaatttt aaaacaatgc ttcctaaact taattctact aattttgaag gtataaaaat
     1981 tataagacca ctttattata taagagaaga acatattata agatttattc aaaatagtgg
     2041 tatatggcca ttaaattgtg catgtatggt tgctgctaaa aaaacaggta ataaaagata
     2101 tgagataaaa gacttaataa agagtttaga atcaaatttt aaaaatgtag aaaaatctat
     2161 atttaaggcg gcagaaaatg taaatcttga ttcagtactt ggttggcaaa aagatgggga
     2221 gaaacattct ttcttagaaa attttgaata aaaatagtta aaaagaatca ctataaacaa
     2281 ttggaaaggg tgtttatatt gaaaagagta tatatagatt ttgaaatgaa tatgccaaat
     2341 tctaaatcaa aaagagcagt atttaattct gatataattg caattggagc agttgtgtat
     2401 gatgaaaaaa caaaaaatat tgataaattt aaatctttaa taagaccagt aagtaatgag
     2461 gagttgtatc ctcatataca agaattaaca catatatctt ctgaagaatt gaagagtgca
     2521 ccaagttatg aagaggtcat gagaaagttt aaaaaatggc ttggcatatt ttctgatatt
     2581 aaaggaatat atacttttgg taatttggat ttgacatgtt tcaataacac agatatgaga
     2641 agtgctaaaa aaaataacca tccaaggttt gtaaataaca ttagagattt atttgtagat
     2701 ataaaagaaa aatatataaa ttgtggtatg agatgtatga actatatatc attgaaaaat
     2761 ttacttgaat ttgttaactt agagtttagt ggagatgccc atgacccatt aaatgatgca
     2821 tataatttat ttattttgga tgaggcaatc acaaataatg tagatataca aaatctttta
     2881 ataatacgtg acataataag accaccattt aacgatataa attctaattt agataattgt
     2941 tttaacaaat ttaaggagtc attatataaa aaagaaggaa attataatat tgtagatttt
     3001 tctgttgaaa taataaaaac tgtgagaatg tacttattaa caatcataga tgtaaatata
     3061 caaaatttag aaattatgaa agatataagt aaaaaaatgg atactataga taaattgaaa
     3121 gatatagagg aaggctattt ttatttatta gaagatgtgt acttcgacat gaaagatgta
     3181 ctagaagatt taatgcttta tagaatgaat gaagatgagt acaagtatga gataaaaaat
     3241 ataataaaaa tgtttgatga agacttagat aatgaaaaaa tatatataaa taaatgtaat
     3301 aacctaaatg ttgttaataa gatataagaa attataaata atagagtaga acttgacaat
     3361 tataacaatt ttttataaaa ctgaatcaag ataaaattaa attaatataa aaataatata
     3421 caataaaaaa tgtagagaga tacttacttt ctacattttt ttattgtata ttgctttagg
     3481 ttt
//
LOCUS       DAYAOX010000291         3477 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.294, whole genome shotgun sequence.
ACCESSION   DAYAOX010000291 DAYAOX010000000
VERSION     DAYAOX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.294"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            72..2183
                     /locus_tag="ACGI57_003486"
     CDS             72..2183
                     /locus_tag="ACGI57_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HFG9718532.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(2381..3346)
                     /locus_tag="ACGI57_003487"
     CDS             complement(2381..3346)
                     /locus_tag="ACGI57_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFG9718533.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
BASE COUNT         1353 a          464 c          581 g         1079 t
ORIGIN      
        1 atgaatttta gatatactta ataagaagta tattgtagaa aaaataaatt taaaaaatta
       61 agggggatga aatgaaaaaa ataagtatat tagtattatc attgataatg acacttacta
      121 tgtgtagtgt aagttcattt gctgattcat caaatgataa ggaaatgaga gctgcttgga
      181 tatctacagt ttataattta gactggccta agactaagaa taatgaagcc aagcaaaaaa
      241 aggaatacac agatttattg gacaaattaa aaagtgtagg aataaataca gcagtggttc
      301 aagtaagacc aaaatcagat gctttatata aatcaaatat aaacccatgg tctgaatatc
      361 ttaccggtac acaaggtaaa gaccctggat atgacccatt accattttta atagaagaag
      421 cacataaaag aggaatggaa tttcatgcat ggtttaatcc atatagaata actatggcag
      481 atgagagcat agataaattg ccagcaaacc atccagcaaa gaaaaatcca agctgggtag
      541 ttaaacatgg aaataagtat tactacgacc caggtttacc agaagttaga aagtacatag
      601 tggatagtat agcagaagta gttcaaaatt acgatataga tggagttcat tttgatgatt
      661 acttctatcc aggagtaagt ttcaatgata ctgcaacata ccaaaaatat ggaaaaggac
      721 aaaataagga tgattggaga agagaaaatg taaatactct acttagagat gtaaaagcgt
      781 ctataaagag tataaagcct aatgttgtat ttggtgtaag cccagcaggt atatggagaa
      841 ataagagtag tgacccaaca ggttcagata catcaggtaa tgaaagttat gtaggtacat
      901 atgcagatac aagagcatgg ataaaacaag gattgattga ctatgtagtt ccacaacttt
      961 actggccaat tggtcttaaa gcagcagatt attctaaatt agtggcatgg tgggctaatg
     1021 aagtaaaagg aacaaatgta gacttataca taggacaagg tatatataag caaggtcaat
     1081 cgagttatgg tggacaaaat atagcaaaag aaatagtaca acaagtaaca ttaaatagaa
     1141 agtatagtga aataaaaggt agtatgtatt tctcagctaa agatatagca aatagtactt
     1201 ctatacaaaa ggatttaaag agtttatatt ctagtagtga agaaccagtt actccaccat
     1261 ctaatgttaa agtggagaaa ttaagaggcg acgaaagata tgacacagca gtagcaataa
     1321 gtaaaaaagg ttgggctaca aattcagata cagtagtatt agtaaatggg tactctatag
     1381 ttgatggtat aacatcaaca ccacttgcta catcaaatga tgctcctata ctattggtaa
     1441 ataaggataa tatacctaca tccactaaga atgagttaaa aagacttaat ccaagtaaag
     1501 ttattttaat tggaggaaat aactctattg gagataaagt tgagagtgag ataaaagata
     1561 cattgtcaaa tgtatcaata aatagagttg gtggttcaga tagatatagt acatctttaa
     1621 tgatagcaaa agaattagta aaaactaatc cagtagaaaa actatatata acaagtggta
     1681 caggtgaagc agattctctt tctatagctt caaaagcagg agaagaaaag caaccaatag
     1741 tattagtatc aaaagataat gtaagtgatg aagtttacaa ttggatatca gatttgaaag
     1801 taaaagatgc ttattttata ggtggtaatc taagtatatc tgattcagta ataaataagc
     1861 tggataaggt cattacaaat gatgtatcta aaaatagaat agctggagaa aatagagaag
     1921 aaacaaatgg aaaagtaata caaaaattct atccaaatgc tgaatattca tcaatgtttg
     1981 taagtaaaag taatcaattg gtagatgcat taacatcagg tcctttagca gctaaattaa
     2041 aatcaccagt agttatgctt ggaaatagtg taacatctgc tcagaaaact gctttagagc
     2101 ctaagaaaac tactctagta tacgaagctg gagatggtat aaatcaaaac acattaaaca
     2161 ctttcttaaa cttagttaag taataaaaaa gttaatttaa aatacagagt attttataaa
     2221 tgtagattaa aatgaaaatt atcatgataa attagaaaat aaaaaagaac tatatgcatt
     2281 ttgtaatcaa gtgtacacag caacttgaaa ataattatgc gatatagttc ttttttagta
     2341 attaatctta tactttttaa taaactttat gttgaatttt ttattgttta aactctatgt
     2401 cattaaatat aaaatcgtgt aatatgtcta aagtatctgg gtcaaatcta agaacccatc
     2461 cagcttttcc atatatacca cctgtactat gaatttcatc atcaatagga aattcaaatt
     2521 gcttaatatt taagtcgccc atttttaaaa cttgagctcc taaagaaagt attgcattag
     2581 gcttcatgtt agttttaaca tatggaagaa ctgcatctaa taaacctgga tatttagtaa
     2641 caggtaaagt ttccgttttc ttgattatag cttgtataat ctgtctctgt ctgccatctc
     2701 tagcaaaggc tgtatcgttg tgtctgatac gtgcaaaaga tagtgcctga tatccattta
     2761 gagtctgctt tccagagttt ctaatgtatt gtatgctacc tttatcatca cttttattcc
     2821 atttataagt ttcaggaata aatttattta gttctcgtat ttctcctttt tcaacagtaa
     2881 cctcaacgcc accaatagca tcaattatat acataaatga ttcgaaatta acacaagcat
     2941 aattttgaat atcaatatta aaattctctt caattgtttg tattaataaa tcagcttgtc
     3001 cataagcata agcatgagta agtttttgtt taccatgacc agggatgtct acatagctat
     3061 cacgagctaa tgatgtaagt ttgatatcat tgtgtttgtt atcaatagta agtatcatca
     3121 tagcatctga ccttgaagaa tcttcatttg gtctagcatc tgttcccata agtagtatgt
     3181 taattatccc atcttcattt ttatggtcat tgttatccaa taaatcacta cttatgctcg
     3241 agtcatgaat agcagataat ttataataaa aatatccata gacacttata gggaatatta
     3301 ctaccaagaa agccagtagt ataacaaatt tctttaattt tgacaaaagt accccttctt
     3361 tcttcttttt cctgtatatt ttttaatagc atacacttaa ttatattaaa gtattgagat
     3421 atattcaata cttgtttcag ttacaaaaaa aagacaatga tataatttat aagaaaa
//
LOCUS       DAYAOX010000292         3435 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.295, whole genome shotgun sequence.
ACCESSION   DAYAOX010000292 DAYAOX010000000
VERSION     DAYAOX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.295"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..1062
                     /locus_tag="ACGI57_003488"
     CDS             <1..1062
                     /locus_tag="ACGI57_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFG9718534.1"
                     /translation="KLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQYKGKIKSAN
                     LSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIKLIKKLNKK
                     GISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPLDVNVLNIG
                     SNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQKLNNNFFD
                     KLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKAHRKPIFFG
                     ELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSVFCVGKIDD
                     EKNFYPSKESIKVIKSWYE"
     gene            1116..2240
                     /gene="wecB"
                     /locus_tag="ACGI57_003489"
     CDS             1116..2240
                     /gene="wecB"
                     /locus_tag="ACGI57_003489"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFG9718535.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2318..3292
                     /locus_tag="ACGI57_003490"
     CDS             2318..3292
                     /locus_tag="ACGI57_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFG9718536.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         1435 a          335 c          601 g         1064 t
ORIGIN      
        1 aaattaaaaa gaagtatttt aatctcatta ataatcttaa tttttatatt tatagtttac
       61 tttgtaagta ttgagttaaa agataggagt ctagtaaata atcaatataa aggtaagatt
      121 aaatcggcca atttatctgt agattatgaa ataaatcaag ttatgaagga tatagataaa
      181 ttgggattga atacagtaaa tgtacctata attataaatg tagagtccat taattctgat
      241 attatgtcaa tagataataa tagtaaagaa aaggcaatta agttaataaa aaaattaaat
      301 aaaaaaggta tttcaacaat attagaggca tatccatgga ttgaaaatgg aaaattgtat
      361 gagactgact ggaaccctat aaataaaaaa agattttttt atacttggca aaatgccata
      421 ttaaatgaac ttataattga tgtagcaaat ccactggatg taaatgtttt aaatattggt
      481 tctaattttg tacatttgga agaatatcaa caaaattggg gtgaaataat agattttgta
      541 caatccaaat ttgacggtct tgtaacgtac agaactaatt ggtggtatac aaaaaaagat
      601 gatttaagta gtaagttgtt ttatgaacaa aagctgaaca ataatttttt tgataagtta
      661 gattttatat ctatagcagc atattttgag ttaagtaata aacctgttaa tacagtggat
      721 gaattaattt ctgccttaca ctcatctact gtaaacaata gaggacaaaa tataaaacaa
      781 gaaatatata acctttataa agctcataga aaacctatat tttttggaga attaggtttt
      841 tcaaatagag aaagtgcaag ttctcagccg tggaatcata ctccttcaaa agctgtaaat
      901 ggcgaagaac aagcaaggtg ttttgaggca tataagaaag tttttgaaaa tgaagactgg
      961 ataaatggtt tttctgtgtt ttgtgtaggt aaaatagatg acgaaaaaaa tttttaccca
     1021 tcaaaagaga gtataaaagt tattaagtct tggtatgaat aattttgaaa gtgtagaatt
     1081 tatatagatt aaatagatga ttaggagaga tataaatgaa tgacatcaag gttatgactg
     1141 tatttggaac tagacctgaa gctatcaaag tagcaccttt aataaaggaa cttgaaaaaa
     1201 gagagaatat aaaaagtatt gtttgcgtta cagctcaaca tagagaaatg cttgaccaag
     1261 ttatagaaac ctttaatata aatgtagact atgatttgga tataatggaa aaaggacaaa
     1321 gcctgaatga tataacatgt aagattttaa ataaacttcc cttgatttta aacaaggaaa
     1381 atccaaatat aatattggta catggagata caaccacaac tctagctaca agtttgactg
     1441 cattttataa taaaactctt gttggtcata tagaagctgg gcttagaacc tatgataaat
     1501 attctccttt tccagaggag ctaaatagac aacttacagg tattattgct gatatgcact
     1561 ttgctcctac taatttggct agaaaaaact tgatttctga aggaaaacct aataataata
     1621 tatttgtaac tggtaatact gctattgatg ctttaaagat gacaataaaa gaaaactata
     1681 atcatcctat aattgatgag attggtaacg ataggatgat tttgttaacc tctcacagaa
     1741 gagaaaattt gggtaaacct atgaaaaata tattcagagc tataaagcga atagtagatg
     1801 actttgaaga tgtacagata gtatatccca tacatttaaa tcctaaaata cgaactatag
     1861 ctgatgagat atttggaaaa tttcctgaaa aaattcatat tatagagcct ttagatgtag
     1921 ctgattttca taacttttta aataaatctt atatgataat gactgatagt ggaggaatac
     1981 aggaagaagc tccatcatta ggtaagccag ttcttgtgtt gcgtgataaa acagaaagaa
     2041 cagaaggaat tgaagcaaag acacttaaat tagtaggtac taatgaagat agaatatata
     2101 attcagtgag cgatttatta ataaataaag ataactatgt tcagatgagt aaagcatcta
     2161 atccatatgg agatggaaat gcaagcaaat atatagtgga tataataatt aaaaaattta
     2221 attgcaaata tttaaattag ataaggccta aaacattaac taattgatat aagatttatg
     2281 ataatattaa ggaatacaat ttttaagggg gagtaatatg gctagaaaat taataaaaaa
     2341 tttgggtaaa agtaaaagtg taaagagagt gaaactttta tttaagaaga tttttattac
     2401 tgtattcata gtagcaagta tagttgctat ttttaatata acgaaatatt ttgaggaatt
     2461 atacaaagta agggatttaa aaagtactaa aattgaatac tatatggatg tggctgatga
     2521 agcaggagat ggaaaggtcc aattaagttg gaaggcctta cttgctatag acatggtaat
     2581 acatgatgaa gatttaagta atataaaaaa gaaagacaca ttggatatag gggaaaagtt
     2641 tatagtagaa gataaaaatg gtaaaggcga aaaggtgtat aaggtaaaaa agtttaataa
     2701 ggtattaagc gaattgaaat ttgactcttc tcaaaaaagt agagcaagaa aatacatgaa
     2761 agatttagaa tacacatacc ttggaaataa acaattagat agtagtgatg aaaaaattaa
     2821 atttataaag aagttagaag actcagctat aagagaatat attgattatg gaatattgcc
     2881 ctctataaca attggacaag ctatattaga atctggttgg ggaaattcta aacttacaaa
     2941 acagagtaat aatttatttg gtataaaagc agataaagca tggaaaggaa aaagtgtaga
     3001 aatttcaact tcagagcatt ataatgaaaa aattgtagct agttttaggt cgtataattc
     3061 attacaagat tctgtcaaag accacagttt atttttaatt aataataaaa gatatagaaa
     3121 acatgggttg tttgaggcaa aagattatat tagtcaagct caagcattag aaaatgcagg
     3181 atatagtaca gcagaagata aaaaaggaaa tcgtatatat gcagagctat taatagatgt
     3241 tattaggagt tataatttgc aattgataga taataaagtt gagacaaaat aattgttaac
     3301 tttatatgag gagataacaa gataagattt ttttctacaa aaagaataga aaaattgtaa
     3361 aaaaaactaa aaaaatgtat tgacttgtta tttaaaaggt gatattatta ttactgtact
     3421 cgaaagagaa caaaa
//
LOCUS       DAYAOX010000293         3410 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.296, whole genome shotgun sequence.
ACCESSION   DAYAOX010000293 DAYAOX010000000
VERSION     DAYAOX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.296"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(155..1426)
                     /gene="serS"
                     /locus_tag="ACGI57_003491"
     CDS             complement(155..1426)
                     /gene="serS"
                     /locus_tag="ACGI57_003491"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFG9718537.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(1552..1757)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1885..2766)
                     /locus_tag="ACGI57_003492"
     CDS             complement(1885..2766)
                     /locus_tag="ACGI57_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFG9718538.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(2778..3287)
                     /locus_tag="ACGI57_003493"
     CDS             complement(2778..3287)
                     /locus_tag="ACGI57_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFG9718539.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT         1100 a          590 c          325 g         1395 t
ORIGIN      
        1 ttagtgtttt atgatgaaaa ttctgctgtg taaattaaca taaaaaaact atgaaattaa
       61 tatttataat aatttcatag tttttttatt tattgtttac tcataatata ttctcttcat
      121 caaagcatac tctatatata caattaatat aaatttattt tataacatca acacccatat
      181 aaggtcttaa ttcttctgga actactacag aaccatctgc ctgctgataa ttttctaata
      241 tagcagccaa acatcttcct attgctaatc cagaaccatt taatgtatga acatattctg
      301 cttttgattt tttatctctt ttaaatctta ttcctgctct tcttgcttgg aaatcttcaa
      361 aatttgaaca agaagatatt tctacatatc tattgtaact tggcatccaa acttctaaat
      421 catatttaaa tgcagctgta aatcccaaat ctcctgtaca tatttttaca actctatatg
      481 gaagacctaa catttgtaac atagtttcag catcatgagt taatgattct aattcattat
      541 aagaatcttc tggtttaaca aatttaacta attcaacttt attaaattga tgttgtctaa
      601 ctaaacctct tgtatctctt cctgctgaac ctgcttcaga tctaaaacaa ggagtatatg
      661 cacagtattt aattggaagc tctgcaacat ctaatatttc attagcatgt atattagtaa
      721 caggaacttc agctgttggt attaagaaat actctaatcc ttctatttta aacatatctt
      781 cttcaaattt aggaagttga ccagtcccta taaaactatt tctattggcc ataaatggag
      841 gtaagacttc cgtatagcca tgcttagctg tatgagtatt taaaaagaaa ttcattaaag
      901 atctttctaa tctagctcct aatcccctat ataaagtaaa ccttgaccca gttattttcc
      961 ctgctctttc aaaatctaaa atacctagac ctgtacctat atcccaatgc gctttatgct
     1021 caaaatcaaa tttagtaggt tctccccaag ttcttatttg aacattgtct tcatctgttt
     1081 caccttgtgg tacctctgga tgaggaacat ttggtattct cattaatgta tattccattt
     1141 ttgcttctac ttcttttact ttctcatcaa taccttttat cttatctgat aactctttca
     1201 atcttgcctt ttcttctgtt acatcttttc cttcttttat taattgaggt atttttttag
     1261 aatttacatt taactcattt ttcataactt caacttcttg aagtatttct cttcttttat
     1321 tatctaattc cactacagca tcaaggtcaa attctctttc acctcttctt tccatagctt
     1381 ttttgatatc atctagattt tctcttattc ttttaatatc taacatatta attcctccta
     1441 ataattttga taatataagt tataattcca acttaattta caaacattat acttgtaaac
     1501 tcttagttta aacaaatttt aaaaataaaa aaagacctcc gtttacccta taaaagggac
     1561 gaaaatcttt tatccgcgtt gccaccctaa ttgactatac taaaatgtat aatcctctca
     1621 gacctataac gtaggtaaac gtctagcttt aactagaagc tctagagtag attcaaaaac
     1681 cattgtaatg agttttcacc atccactcat tctctgaata caatctgttt tttactattt
     1741 tccttcacag ctttttaact aattgtttta tttgataatc attatattat atatattaac
     1801 atctttcaat actattattc aaattttttt agaattttct ttaatttttt atattaaatt
     1861 cattttttct atttaattaa aaccttatct tttatccatt gtatttagat tacctgtatt
     1921 attaaataat aatgaagaag gaacaaatgg aacttttata tcattctcat caaatttatt
     1981 ttttaccttt tccattacat caaaatatac atcccagtaa tcatcagttt ttacccaagg
     2041 tctagcaact atttgcatgg tgttatttcc tatagagttt aaacctatta taggctttgg
     2101 atcatttaat attctttttt cgtttgtaaa aatttcattt aatatagact taactacatc
     2161 tatatcacta tcatatgaaa tattatatga aaaatctatt cttcttttgt tttgatgtgt
     2221 gtaattaatt atattattac ttgtcagttg aaaattaggt ataattatag ttttattatc
     2281 tggcgtctta agaacagtgc taaatatttg tatgtcagaa actgtacctt ctacacctga
     2341 accttgaata taatcaccta tattaaatgg cttaaaaaat aaaataatca taccagagcc
     2401 taaatttgat aatatttctt taaaagctaa acctatacta aatccagcag ctcctaacac
     2461 agctacaact gatgtagttt gtatccctat aatacctaag cttatcaatg atataataga
     2521 taacatagat atatatattc catatacaat aaaattattt acacctttac tcattccata
     2581 tttgattaga aactttgaaa ccatccttct tactatttta gatatataaa tacctatcac
     2641 aaatacaatt attgcatata taatatttgg tatattatca ctaaaattat cgagcatatt
     2701 ttctaaagtt ctcttagtta tattgctatt tgttgaggca gtcttagcca atgataaaaa
     2761 attcattcaa ctcctcctta tgataatatt ccgttgtcct ttaactcttt tactatagct
     2821 tctatagttt cttcttttat tgcctcaatt gtatgaatat ggctacattc tgtcagaacc
     2881 gataattgtt taaattcatt tgtctcaact ttcttcataa agttgtctat atccatttta
     2941 gaataaatat taagttctgc agttatctga ccatatgttg gatgatttac gatgacatct
     3001 tttataatac cacctaaatc cacaattatc tttaattcat cataaatttc tttagaatcc
     3061 ttatgatttt tacactttat ttgttttgta aaatacggtt gattatatat tatatatcct
     3121 tgaggtgtag ctattatttc aaatcctcta gctcttataa gggctatatc ttgaactatt
     3181 acttgcctac ttacatttaa tagttcagct agtgttccac cttttacagg atgcttagat
     3241 tcttttaaaa tatctattaa tttacttctt ctttcattag aattcatatt acaccttctt
     3301 tccaaataaa aactttacct atactatata attttatatt ttatcttatt ataaatcaat
     3361 attgacttta tattctattt aatcttaact tttataataa ataaaaaaga
//
LOCUS       DAYAOX010000294         3338 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.297, whole genome shotgun sequence.
ACCESSION   DAYAOX010000294 DAYAOX010000000
VERSION     DAYAOX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.297"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(67..513)
                     /locus_tag="ACGI57_003494"
     CDS             complement(67..513)
                     /locus_tag="ACGI57_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718540.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            788..1066
                     /locus_tag="ACGI57_003495"
     CDS             788..1066
                     /locus_tag="ACGI57_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718541.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     ILVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(1195..1926)
                     /locus_tag="ACGI57_003496"
     CDS             complement(1195..1926)
                     /locus_tag="ACGI57_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718542.1"
                     /translation="MNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDANLRAVV
                     KRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEVPKEAL
                     SLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHNVDIAK
                     GESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGNKTGKA
                     VVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(2070..2714)
                     /gene="trmB"
                     /locus_tag="ACGI57_003497"
     CDS             complement(2070..2714)
                     /gene="trmB"
                     /locus_tag="ACGI57_003497"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFG9718543.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(2852..3172)
                     /locus_tag="ACGI57_003498"
     CDS             complement(2852..3172)
                     /locus_tag="ACGI57_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HFG9718544.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT         1172 a          520 c          348 g         1298 t
ORIGIN      
        1 caaaatacaa gagaatacaa attaatacct cgggaatttt taagacaaat accaatatta
       61 tgcaatttag acttcgcttt ctttcaaatt agtatcattt tctttatttt tacataggat
      121 gtactttaat ttagctatca aaacattgct atataggtta aaagttatta gaattgcagg
      181 aattataaag taaactctag cacctataaa accaaaaact actgtaataa gtattaatct
      241 tttatttaaa gtaaaaaaag gtatattaaa aatacttttg tcggatgttt ctggagtata
      301 agaatatata actccaaaaa tcaaagtaag catacaaagt ggaagtagtg tataacttga
      361 aattattccc aaaaatccta tactttcaaa atatttgcta gcactatttg tataaacaaa
      421 catgtatcca tttaaatatg cgcatataca tgatataatg ccaaaagttc cccctaagga
      481 ttgagtgtac caattaaaaa atttagattc catatacctc acctcctaaa actgtatata
      541 taatgatatg catttaaata gtaaaatata tctttatatg tttctgaata aaattttgtt
      601 aatttcatta atttatgttt aagaaatttg aagtatagtg tagtatatat taatgtagat
      661 gaagttttat agtacttatt tcatagtgct taatcaagtt atcttattat ggattcaaaa
      721 gttgtttgta gggttttata ttttgttgag ctgaaaaatt tattataaat gaagggatgt
      781 tttcgctttg gtagatacat tagaatttgg tcttaaaatt ttatttttta tattatcaat
      841 aatttggatg ggtaaaataa tgattttaag aactgataag caaatagtaa tcaatccttt
      901 gcttattggt atatctgcaa tccttgtgat gttgcatacc tcacagagta acattgaatt
      961 ttttggatta gatgtccaat atataagaat agttttgtat ataatatatt cactgataat
     1021 tttaatagga atatgggcta ctaacagaag aaatggaatt ttttagataa tctatttcta
     1081 taaaatttaa aggttgctag taacaaatca ctaatattag cgttctgtta ctaacaacct
     1141 ttattttgat tatacactta aattgaaaat agaatgattg aataagttga cagattattt
     1201 tacattgaca gtaattagaa cttcttgaga tgtatttaaa tcatttttgt cgcttagtat
     1261 aacttttacc actgctttac ctgttttatt acctttaatt atcatatctt cacatgaaag
     1321 tacactctta tcacttacag atacaataaa atttaaatct tttggaaggt tatcagtaac
     1381 ttcccagtat ttataataac tatcaatttt cttcgtaatt atttttctca aatcaatact
     1441 ttcacctttt gctatatcaa cattatgata tttagagtcc agtttatact gattatcttt
     1501 tgtatcaata aaatttaaat cgcaatttac atccacacca ttatgagaaa gagaatttac
     1561 tacatcgtca tcaatcaaat aattattggc aagatttatt gaatccatat cttttagact
     1621 taaagcttcc ttaggaacct cttttatatt attattccaa aaaataaaat tgtctacatt
     1681 ttcaagatat gatatacctt ttaaatttga aatactctca ccagttgcat aaaattctcc
     1741 atctaaagcc tttatattgc ttatagtcat ctcatctgga tttatatatc tttttacaac
     1801 tgctcttaaa tttgcatcag gaaaaatatc tttaagttga ttttcgctca aaaccttatc
     1861 atttgaattt attggactta aagctgctgc agaaataata aatggtatta aattagctaa
     1921 attcatagta ccctcccaca taaatttttt attataataa tttaatttct atgagagggt
     1981 tctgtttcct tgttatatat acctactttc gtacacttta ttaatataaa ttaaaataaa
     2041 tttctactat taaacttata aacatattac tagttacgtt ttttagcttc acatctaaat
     2101 attcgcatac cttgtgacat aaatttgtct tcatactctg ttgtaacatt attttcatac
     2161 tcactatttc ctaaatcaag agaaatattc tttaatagcc aattatttgc agcaatttca
     2221 tttaaactaa actcgaataa tttttcatta tctgttttaa agtgaatttc gccatcatct
     2281 tccaaaaccc tattgtacat ttctaaaaat ccagaatgag tgagtcttct tttactccat
     2341 ctatttttag gccatgggtc acagaaattt atataaattc tcgataattc tttagcttca
     2401 aagtaatcca aaatattgct tacatctccc cataaaaata gtatattatt caaattagaa
     2461 gcatctgctt tttcaacagc ttttaaaaga acttcttcct taagttccat ggcaatataa
     2521 tttatgtcag ggttctgctt agctaaagtt gttataaact taccttttcc agtcccaaat
     2581 tcaacatgta ttggattatc atttctaaac tttaaattcc acttgccttt taatttgtct
     2641 atatcgtctc ttaaaacata ttttgtataa cttaaaagct tttcatctgc accttttttt
     2701 cgtcttcttc tcacaataat tctccttaca tataaaaaat tagtaaaata catttttatt
     2761 atacattatc tcaccaaaaa ataaaaagaa ctctcttaaa atagaaattt atttctataa
     2821 agagagttct aaacttacta aaaacttaat cttagtggca ccctgaacaa ccagagcatc
     2881 ctcctccacc ttgatctttc atattttcag gaataactct aaatccaaat cctgtatctt
     2941 ctattattat atctccatac tgactatatt catcagagct cattacaaat tgtaattctc
     3001 caacttcata agttaagtca tcttcttttt tctcatctaa agctaatcca aaagaaggac
     3061 ctccacaacc aacacctgca aagtatactc ttatattatt aggtttatct tgattgtctt
     3121 ttaagatact ttttaaagta tcaatagctg tttctggtaa agtaattttc atcaaatcat
     3181 tccctttcaa atattttatt aaaacaatat ctattatata cccatacaag gtacataatc
     3241 aattagtttt tgcatatttt actaaaactt ctacctaata ttacctaagg ctttaaaata
     3301 ttttatataa tattttatta ccttaaataa atacccta
//
LOCUS       DAYAOX010000295         3311 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.298, whole genome shotgun sequence.
ACCESSION   DAYAOX010000295 DAYAOX010000000
VERSION     DAYAOX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.298"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            86..1162
                     /locus_tag="ACGI57_003499"
     CDS             86..1162
                     /locus_tag="ACGI57_003499"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9718545.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTDGGFKSDDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            1229..3223
                     /gene="bclA3"
                     /locus_tag="ACGI57_003500"
     CDS             1229..3223
                     /gene="bclA3"
                     /locus_tag="ACGI57_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HFG9718546.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAVGATGIGIT
                     GPTGPTGATGADGATGFGVTGPTGPTGATGIGITGATGLIGPTGATGATGATGPTGVT
                     GATGAAGLIGPTGATGATGADGATGATGATGATGPTGADGLVGPTGATGATGADGLVG
                     PTGPTGATGVGITGATGATGATGPTGADGLVGPTGATGATGADGVAGPTGATGATGNT
                     GADGATGPTGATGPTGADGLVGPTGATGATGLAGATGATGPIGATGPTGADGATGATG
                     ATGPTGADGLVGPTGATGATGATGATGPTGATGATGATGPTGASAIIPFASGIPLSLT
                     TIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRAGTITSISAYF
                     STTAALSLVGSTVTITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSISSGIVT
                     GLNIAATAETRFLLVFTATASGLSLVNTVAGYASAGIAIN"
BASE COUNT         1203 a          574 c          797 g          737 t
ORIGIN      
        1 ttttataaat ttaataacaa aacaagagaa aataccatat attacttatg tctgcaatat
       61 atagaagaca aaggagttgt acattatgat tacaataagt ttgtgcatga ttgttaagaa
      121 tgaggaagaa gttataggca gatgcttaga atgtgtaaag gatatagtag acgaaattat
      181 aattgtagat accggttcta cagattccac aaaagaaata gtctcaaaat atacagacat
      241 ggtgtacgat ttcgaatgga tagatgattt ctctgctgca agaaattttt cattttctaa
      301 agcttctaaa gactatacta tgtggcttga tgcagatgac ataattttag aagctgatag
      361 agaaaaatta ttaaagatta aagaaagctt ggatacttcc attgatattg ttatggcaaa
      421 atacaatgta tcctttgatg aaaatggtaa cccaactctc tcatattata gagaacgatt
      481 gtttaaaaga agttctaatc ataaatgggt tgaccctatt catgaagtag tacctctttt
      541 tggagaggta ttttattctg acattgcaat atctcataaa aagcttcata gacaggaccc
      601 tctaagaaat cttagaattt ttgaaaaaat gatttcagaa ggaaaaacat tggagccaag
      661 acaccaattc tattattcta gagagttgta ctataatgct aggtataaag aagcaataga
      721 aggttttact aagtttttag actcatcaag aggatggatt gaggattgta tcagtgcttg
      781 cagagattta gccacatgtt attacttaat taatgatgaa aaaagtgcct tatattcatt
      841 gtttagaagt ttcgaatttg atgagccaag agcagaaatt tgttgtgaca taggaaagca
      901 tatgtttgat agacaaaaat ataaagaagc cattttttgg tataaggtag cattaacaag
      961 agataagaat gatactgatg gaggctttaa gtcagatgat tgttatggat atataccatc
     1021 tatacaatta agtgtctgtt atgacaggtt aggagaatca gataaagcta tttattatca
     1081 tgaaaaaaca aaagaaatca aacctaatga tagtgctgtt ttgcacaatg aaaattattt
     1141 tagtaaattt aaaaatagtt gatgggtcac aatcatacta aatttgacat attatatata
     1201 gagcttaaaa gctcaaatat atcaggaggt gcttttaata atgagtagaa ataaatattt
     1261 tggaccattt gatgataatg attacaacaa tggctatgat aaatatgatg attgtaacaa
     1321 tggtcgtgat gattataata gctgtgattg ccatcattgc tgtccaccat catgtgtagg
     1381 tccaacaggc ccaatgggtc caagaggtag aaccggccca acaggtccaa cgggtccaac
     1441 aggtccaggt gtaggggcaa caggcccaac aggaccaacc ggtccgactg gtccaacagg
     1501 aaatacaggg aatacaggag caacaggatt aagaggtcca acaggagcaa caggggcaac
     1561 aggcccaaca ggagcgacag gagctatagg atttggagta acaggtccaa caggcccaac
     1621 aggagcgaca ggagcaacag gagcagatgg agtaacaggt ccaacaggtc caacgggagc
     1681 aacaggagca gatggaataa caggtccaac aggagcaaca ggggcaacag gatttggagt
     1741 aacaggtcca acaggcccaa caggagcaac aggagtagga gtaacaggag caacaggatt
     1801 aataggtcca acaggagcaa caggaacacc tggagcgaca ggcccaacag gagcagtagg
     1861 agcaacagga ataggaataa caggtccaac aggtccaaca ggagcaacag gagcagacgg
     1921 agcaacagga tttggagtga caggcccaac aggtccaaca ggagcaacag gaataggaat
     1981 aacaggagca acagggttaa taggtccaac cggagcaacg ggagcaacag gagcaacagg
     2041 cccaacagga gtaacagggg caacaggagc agcaggacta ataggaccaa ccggggcaac
     2101 aggagcaacc ggagcagatg gagcaaccgg agcgacaggg gcaacaggag caacaggtcc
     2161 aacaggagca gatggattag taggtccaac aggagcaaca ggagcaacag gagcagatgg
     2221 attagtaggc ccaacaggtc caacaggggc aacaggagta ggaataacag gagcaacagg
     2281 agcaacagga gcgacaggtc caacaggggc agatggatta gtaggtccaa caggagcgac
     2341 gggagcaaca ggagcagatg gagtagcagg tccaacagga gcaacaggag caacaggaaa
     2401 tacaggagca gatggagcaa caggtccaac aggggcaaca ggtccaacag gagcagacgg
     2461 attagtaggt ccaacaggag caacaggagc aacaggatta gcaggagcaa caggagcaac
     2521 aggcccaata ggagcaacag gtccaacagg agcagatgga gcaacagggg caacaggagc
     2581 aacaggtcca acaggggcag atggattagt aggtccaaca ggagcaaccg gagcaaccgg
     2641 agcgaccgga gcaacaggtc caacaggagc aacaggagca acaggagcaa caggtccaac
     2701 aggtgctagt gcaataatac cttttgcatc aggtatacca ctatcactta caactatagc
     2761 tggaggatta gtaggtacac caggttttgt tggctttggt agttcagctc caggattaag
     2821 tatagttggt ggagtaatag accttacaaa cgcagcagga acattgacta actttgcatt
     2881 ttcaatgcca agagctggaa caataacatc tatttcagca tacttcagta caacagcagc
     2941 actttcactt gttggctcaa cagttacaat tacagcaaca ctttaccaat ctactgcacc
     3001 aaataactca tttacagctg taccaggagc gacagttaca ctagctccac cacttacagg
     3061 aatattatca gttggttcaa tttctagtgg aattgtaaca ggattaaata tagcagcaac
     3121 agcagaaact cgattcttac tagtatttac tgcaacagct tcaggtcttt cattagttaa
     3181 tactgtagca ggatatgcaa gtgcaggaat tgcaataaat tagattagat aagaaaaggc
     3241 ttctaatatc gtattgatat taggagcttt tttaagaatg attgatgaac atagttttaa
     3301 tattaattag t
//
LOCUS       DAYAOX010000296         3281 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.299, whole genome shotgun sequence.
ACCESSION   DAYAOX010000296 DAYAOX010000000
VERSION     DAYAOX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.299"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            141..518
                     /locus_tag="ACGI57_003501"
     CDS             141..518
                     /locus_tag="ACGI57_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9718547.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            complement(955..1218)
                     /locus_tag="ACGI57_003502"
     CDS             complement(955..1218)
                     /locus_tag="ACGI57_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718548.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            1534..2109
                     /locus_tag="ACGI57_003503"
     CDS             1534..2109
                     /locus_tag="ACGI57_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718549.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            2207..3205
                     /locus_tag="ACGI57_003504"
     CDS             2207..3205
                     /locus_tag="ACGI57_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HFG9718550.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
BASE COUNT         1312 a          280 c          508 g         1181 t
ORIGIN      
        1 gtgtgtttaa ttttgaataa aaatataaat tttttgtatt aataaatttg gatgagatat
       61 tttaaataaa acctttgact cgaaaagtct attaatatag tatttaatta tgtagaagtt
      121 tgagaggagt gtaaaatcat atgactatta ctaagatacc accagcagaa ttaaaagtca
      181 tgaaatttat ttggagttca gattttacag tagcatcaaa agatgtcatt gaagctatgg
      241 aaaaactata taattggaag caaactacaa cactaacact tttatcaaga ttagtaaata
      301 agcaattctt aagtgcaaga aaaattgata gacatacaca ttatacagtt cttattggag
      361 aagaagagta tttacatttt gaaacaaaaa cttttttaga taatatgcat ggaagttcta
      421 ttgagagttt tatgaaatcc ttgttcaaaa agggggtaaa tgaagaagaa ttgaactctg
      481 ttgaaaaatg ggttaaggat attaaaaaag atgaataatt tggtgtagtt aattatcata
      541 aaataaatag tttttacaat aattagttaa atgaatcaaa tataaagttt tatgtaaaat
      601 ttgtgatttt tataaatatt tttgtcttgt tatttaatat taatacaata agtttgatgt
      661 aaaatcaaac ctgtttgaat ttaatttata ttgggtttgt cattaatatt attttagttt
      721 gatattgtat acgaatttaa aagtcagaat ttttgtatat taatcatgta aattaattgt
      781 tttagatata aagggcataa ttttattttt gttttgatta tttctagggt aagataagta
      841 tacaatagat tgtattaaac atgcttatat tgatagattt gagataaata ttgagatata
      901 ttaataatta ttaatataaa aaagttccta gtttttagga acttttttat ttgattaata
      961 cagagtatga gtttttgctt cagctatagc tcttgctgca cttttaggtt tttcagtatt
     1021 ttcttttttg taaaatttaa tcacttcttc taaatcttta ttttcaatat tatgtttttt
     1081 gttttcaaga atataattta caattgattc atttatcaaa tctgtcaaaa tattattttc
     1141 gtcattagtt atcttagaca tttcttgtaa taaaatttca ttaacttcta ctgtaatttg
     1201 ttttgtattt tttatcatat caaccacctc ttaagaaaat tatatcatct caaaaaaata
     1261 aattcaatat gttttaaaat tatagaattt taattcttaa attctaaaaa agagaaaatt
     1321 agataaagta gttttagatt ttaaaagatt aataaaaaat atttaaagat aaaagcttta
     1381 ttctatatga ttctaagata gaggatttat aagtgtttaa ggtgaattaa atataagaca
     1441 acttcttaaa gcaaatgggt gtggtattta aatctaaatg tagacatata atattaaata
     1501 tgctaatata tcaaaataat aaggggggaa tttatgaagc ttaaagaagc attgattaca
     1561 tgtgttgtat gtagtgtatg tttaactcca ttattggtaa atggagaaac aaaaatagat
     1621 gcagatcatt gtaaagaatc tatagtaaat atagatacta tagtaaagtc aggtaaaagc
     1681 tttaattcca agtataatca aggtatggaa gattataaaa atagtgatga gtataagttg
     1741 aaagtacaaa aggagaaact acaaaatcag ttaggtatta aaataaagaa gttagtacct
     1801 ataacttttg aagttagtta ttacacaagt cttaatcaag aaaatggata tggagcgata
     1861 actgctacag gagatgattt agaagatggt tttgtagcaa acaatcactt aagttttgga
     1921 actaagatta ttgtagatgg aaaattaaaa actgttatgg atagggggtc tagtaagtat
     1981 tttagtaatt ctaatgcaat agatgtattt gtgcccaaat ggagtaatga aagtgaatca
     2041 gagtactatg atagagtcaa taatatggga agacattaca aaaaaggcta cattattgtt
     2101 gaagaatgat gttctttgaa gtaatatgta ttgattgtag tatttaatat atatataata
     2161 aatataaaac tattagtaca atgagatata ttactaggag ggcaatatgg aacaatcgtt
     2221 tataaatgga aaagtaaact tattaataga aaattacaaa gcattaaatg aagttaaagg
     2281 ttcttggcaa atgggattga tacaacatag ttgtgcactt gcatttaccc ttaagaacaa
     2341 gagaataagt cctagattag tggaagaacg aatagagctt attaaaaaaa atactggatt
     2401 attctctaat tttagaggat ataatatgtt ttatatggca acactacttt cgtttgaatc
     2461 aaatccagaa agtagtttta agatgatttt agatatatac aaagaactta aaagtgaaaa
     2521 gttttggggc gatacatatt taccacttac agcatcaata gtttatgaaa atagagagaa
     2581 aatggattat cttacatgta tatcaaaaat gaaaataata tatgattaca tgaggaaaaa
     2641 acatccattt ctaacttcta gtgatgacta ttgtaatata gcacttattg caattcattc
     2701 aaaaaattta gatgaagatt tagaatacat agaaaaatgt tatgaatttc taaatgaaaa
     2761 tggtttttat aaaggaaatg atttacaagc attatcccaa attcttttat ttagtgatga
     2821 tagaactatg cttaaatgta aaaagactat tgaacttaaa aaagcattta aagaaaatga
     2881 ttgtaaaatg aattattatg gatatccaat aataggagca atttcattat tagattatag
     2941 agaagatgaa ataatagaag agataaaaaa tgtttcaaat aaacttaaag aagaaaaagg
     3001 ctttggaaat tggtctttag gaaaaagtaa tagagtaatg attagttctg ctattgtagc
     3061 ttcaatttat gcagatttga tacgaaatga gaataatata ggctctataa ctaataatat
     3121 atttttaaat attgttgttg ctattcaaat tgcatgtgta atggcagcaa caagtgcagc
     3181 tatagcatct tcttcatcaa attaataaga tttattgaag ttggatatat taaatttaat
     3241 aatacaattt atatgtttaa aaagaagata gtattacatt t
//
LOCUS       DAYAOX010000297         3281 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.300, whole genome shotgun sequence.
ACCESSION   DAYAOX010000297 DAYAOX010000000
VERSION     DAYAOX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.300"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            145..1899
                     /locus_tag="ACGI57_003505"
     CDS             145..1899
                     /locus_tag="ACGI57_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HFG9718551.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLIMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            2283..3269
                     /locus_tag="ACGI57_003506"
     CDS             2283..3269
                     /locus_tag="ACGI57_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9718552.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
BASE COUNT         1435 a          281 c          562 g         1003 t
ORIGIN      
        1 tttatataaa taagctaaga tgattgatat aaagttactg ataattttaa ggtaatatat
       61 acgttactat aatattatgt tattatttgt aaataagggg ataatttata agcaaattaa
      121 agaccttaat ttacaggagg aatagtgtat attatgaata aaaaattcaa aaaattcagt
      181 aaaaaactaa gtatattaat tttagttttg attatgtttc tttttaataa tttaactgca
      241 aatgcacaga ttaaagaaga ttcaataata ggaattgata gatatgaaac agcaggactt
      301 atagcagata gacaaactta tgatactgta atattagtta atggagataa aaatttatct
      361 gatgggcttt catcaagtgg attagctgga gctattaacg caccaatact tttgactaaa
      421 aaaagtgaaa taccaaaagc tactttaaat agactagata ataagagttt aaatgttgta
      481 aaaaaagtat atataattgg tggttataat actattgaaa attcagtaga aaaagatata
      541 aaaagaaaag gtattcaagt ggaaagaata aatggtaaca atagaattga aactagttat
      601 aatgtagcta aaaaaataaa tgaagtaagt agtgtaaaag aggtaatgct tacaaatgga
      661 tttgtaggag aggctgatgc tatgagtata gcacctgtag ttgcaaaaaa taaaggagct
      721 ataatactta cagatgggaa atcagttcca tttaagactg aaagtttaaa tgtttacgct
      781 ataggtggca aatctgcgat aaatgaggat ttagtaaatg aaactaaagc aactagaatc
      841 ggaggaaatg atagatttga gaccaataaa aaggtaatgg aaaagtttta taatggagcg
      901 acagattttt atataacaaa tggatatcaa ttagtagatg cactaacatt atctccttta
      961 gccaaagaaa aaccaatcgt tttagttgct gatggaagta ataaaggtat tttaaaagga
     1021 gctaaaagta taacaaaagt tggaggtatt gatgccaata tatataaaca gtgtttagat
     1081 atgattaaat ataataatat ggatatgact ccaaatattt taaaacactt aacttcagtt
     1141 gaaggaaatg aagtttctga aatacttata gaatcagata atttaggtgt ggttgttgaa
     1201 aagacgaata ccaataaatt tgagtttgat tacgtaagtg taactaatga aaaaaattgt
     1261 actttttctg tatataaaga aaattcttct aataatataa aaaatggaaa gctagttgta
     1321 agtgctaaga aaaaaataga taagaaagat agtgatttgc aagatatgaa tggcgacaat
     1381 atgataaatg caaataaaga taggatggta aatgtgataa aaattggagt tcccgataaa
     1441 atgtattcta attttaaagt agatgttgaa agtggttcag tagaacttta taatattaaa
     1501 ggaggagcta ctgttgatgt caatgacggg attgctaaca ttgttgataa caatgtaaca
     1561 aatcctttca atataaatac taatagtggg atttcaggtg taacagctga aactatatca
     1621 tctgaaataa aatttagagc aaacaatgga attgtaaatg taacagctac gaatataaat
     1681 ggcaatataa gtttatttgg aagcgataat aaaggtacct ttgatggaat atttaaatta
     1741 aatctgaaaa aagaaccatc aaatttacac ttgaagttaa ttggaaatgg gataaataaa
     1801 ttgccagaag gttggtctaa agattatatt ttaggaaatg gacaaccaat tattgaagtt
     1861 aaaaataatg gcataaataa tatatcaatt ggagaataaa aacatatgaa aataaagttt
     1921 atatagtttt aaaaattata gaattttaaa atatttatat atattcttta aataaaaaat
     1981 tattttttaa gtgataatta agtgtttatc gataaggcta tggttgtaaa aatcatagcc
     2041 ttatttttat attataaata aattagagta tttatcaatt catagtagat aaaattgaaa
     2101 ttaaatataa caacaaaaaa tgaaaattac aattaattta atgttaaaga ctcaattatt
     2161 tggatgtgga atggatagta caagtagtat ataaaaagta ttgactggac aaataactat
     2221 attttatttt aaatatataa tataatgtaa taaaatatat caaatgtaga aggtgataac
     2281 ctatgaaaaa aataacaata aatgatattg ctaacttggc aggagtatct aaaagtactg
     2341 tatcaagata tctaaataat aaagatatca gtgattctac taaagagaaa ataaagacaa
     2401 taatagatga atatggatat gagccgaatg catttgctca aagtttgaga gctaaaaaaa
     2461 catactttat tggtattata acaccgtgtt tagattcttt tgtaaaatct aaaattatga
     2521 tggcaataga tgaagaactc aaagaattaa aatatacatc actaattatt aatactaata
     2581 gaaaaataag gtcagaaata gatagtatat caaaattagc tagtctaaaa gttgatggaa
     2641 taatactaat tggtaccgaa ataactaaag aacataagaa tgaaattgaa aagttagata
     2701 taccgatagt tgtagttgga caaaaagttg atggtatcaa tagcatagtg aatgatgatt
     2761 atggagcagg atataaaatg ggtcaatata ttgctaataa aggttacaaa aacattgtat
     2821 atttaggtgt tgatgaaagt gatatatcag ttggtttaaa tagaaaaaat ggagtactta
     2881 atgggttaaa agataaaggg tatgatgcaa aagtatttta tactgacttt gaccaagaaa
     2941 cgtctataca aagaagtgga gaaatgctag aaagtgaaaa tccagatata ataatatgtg
     3001 caactgataa tatagcaata gcaacaatga aagaaatcaa taagagaggt aaaaatatac
     3061 cacaagatat atctgtagct ggatttgggg gatatgatgt attgtctatt ataagcccta
     3121 aacttacaac aataaagttt gaaaataaaa atgcaggtaa agtagcagca aatacgatag
     3181 taaatttaat acaagaaaga aaagaaccac tattaaagga gattaaattt gaattaatag
     3241 aaggtgaaag tacaataaat aaaaattaag atacacatta c
//
LOCUS       DAYAOX010000298         3259 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.301, whole genome shotgun sequence.
ACCESSION   DAYAOX010000298 DAYAOX010000000
VERSION     DAYAOX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.301"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(109..1467)
                     /locus_tag="ACGI57_003507"
     CDS             complement(109..1467)
                     /locus_tag="ACGI57_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9718553.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            1915..2367
                     /locus_tag="ACGI57_003508"
     CDS             1915..2367
                     /locus_tag="ACGI57_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HFG9718554.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(2524..3141)
                     /locus_tag="ACGI57_003509"
     CDS             complement(2524..3141)
                     /locus_tag="ACGI57_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HFG9718555.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
BASE COUNT         1102 a          559 c          329 g         1269 t
ORIGIN      
        1 atttatgtaa tttaatcacc ttatatatca tacttataat tatgataata actacataaa
       61 aaatcacttc taaatagagt tatccatcta aaagtgattt catatattct atgcaacttt
      121 gtgttttagc cactttgttc ctcttaatct taaacaatat aaagtccctc taacaagcca
      181 atcagtatac atacctatcc aaattcctaa cactccaaat tctaatacaa cccccaatac
      241 gtaacctgta aaaattctaa aaatccacat acctattact gcagtcatca ttgtatatct
      301 agtatcaccg gctcccttta gccctgctga taatacaaag gatataggcc atattaccat
      361 agctaaacaa tcacttcgaa ttaattgtgt tgagatgtca attacactag cattttgagt
      421 atacatactt gaaatccagg gtgcaattgg tataaatata gctcctacta taaataaaca
      481 aattgttgca aatttagtta aatataataa agtactctta gctcctttaa tatctccacg
      541 tcctatatac tgtcccacca aggttgtagc agacaaagct aaagcattcc caattacatt
      601 tattatggat gcaattgaca ttcctattgc attggatgca attgctgatg ttcccatagt
      661 aactataaat acttgaacaa tcaatttacc tgcattaaaa agtagttgtt caactccagc
      721 tggtattcct atattaaata tagacttttg agtttcaaca tcaatcttaa atggaaatat
      781 tttacccata gatataaatt cgtttccttt aaataaagct cttataatta ctatacatcc
      841 aattattcta gctatagcaa tagatatagc tgcacctgtt atgccatagg atggtgtttg
      901 caaatttatt cctagaggat ttaaatcaat accatatata agtatatatc ccaatgttac
      961 atttactata ttcataaaca tagtcacata cataggtttc tttacatctc cacaccctct
     1021 taaaatacca tttgctattt gttgtgtcgc aataaatgga tacgtaaata atgtaaactc
     1081 tatgtataat tttgcatttg attttaccaa ttcttctgcg gaaccataaa ataaattaat
     1141 tacaccagtt ctaaaaaccc acatcaatat tgttattgta aaggataata ttactccaga
     1201 cactagagcc tgcttaatag tttcattcac cttttcacta tctttttttc ctatttgttg
     1261 agctacaaca acagtagctc caacagatag tgcagcaaat acagatataa atatattgtt
     1321 tattgaatca accattccaa ctgcagatac agcttcttcc ccaatatgac ctgccatcat
     1381 tgtattaaat actcctaaaa gcatgacaaa cgtctgctct atcattatag gaattgctaa
     1441 ctttataaca tttttcctta taaacatccc tattcctctt ttccctcttt atataaattt
     1501 aatttttaat tagtttatcg aactgtaaat tgacaatgca aagtttagaa ttgtattgtt
     1561 cgcattataa tatactgctt tcaaataata taaatcttgt ttaatatcat ttgaacacag
     1621 tatatcttta taattatctt atgtttatta ttttttatgg ataccacatt taaaatattt
     1681 attaatttaa tataaatatt ttccgttatt attaaaagta gctatatttc gttaatattg
     1741 tatcactatc gtgtagcaat aacaataagt acattttata tattttttat aatattattt
     1801 aatcatccaa acttttacca ctttttcaat atataaaata ttacacttta gatgtaacaa
     1861 aatcgcatcc atttaaatat tgtattttat agactaattt taggatgtga tactttggtt
     1921 aatttaaatg caaataatct aaatgtaaat atggtttatc ctatttcttc cacagcaccc
     1981 atacgtttca gaagatatac tgtttcatat ttagaaaaca tggaaaatat ttatttaact
     2041 atagcaacat actttgatac acttggttta ggcacaatga atattgacaa agtttatggt
     2101 caatgggctt ggtttactga taatatatat gatttaaatc tatttgtttt tgtaggaaat
     2161 tatccttatc aaattgctaa aaacagatat gatacatttg tagatatttt gccagtagct
     2221 attacttcta ttgtcaatgg agatagagta tttttaattt caaatccttg tttgttaaat
     2281 tcaaaaatta cagttagatt tatatctagt catcctactc ttaatagaac agttgattat
     2341 ggcaatatta gagattttat tttttaaaac tgcaacatga tgtgctatta aaaaataccc
     2401 taaatttaat gatattatta aataactaga atatttttaa ttggcacatc atgcctagct
     2461 attataaatt cataatatta ttttacttaa gataagtact gtattttatc tatttgtata
     2521 tttttattta cctagtttct cagttctata tcctatttct ttattagcta gcttataacc
     2581 ttcttgtatc atttcttctc tcgttaatgc actccctaat aaattgtcaa tactcagttg
     2641 ctttctatca ctaaaatact tatccaataa tgattcctct atatcttcct gatatgccaa
     2701 atttccttca tgcattatat attttacatc aaaaccttct ttatttaata cttttcccac
     2761 caagatactt ctatgacatc ttataggttc ttgcatagct cctaataaaa ctatcctata
     2821 acccatttga caaccctttt ttaatctttc tattccgttt aaaaaaatat cttcttttgc
     2881 tactttctca aaatctgcat aaccttcttg cgtatataca tctttattag ttcttttagc
     2941 tccaaattct tttcccatgt atatatataa aaatccggaa tttctaagag attcgttaaa
     3001 tgcctcttta ttgtattgaa tattatattt agaatatgga gtttctctaa tgtcaactac
     3061 acaatttatg tcatattttt ttatcatatt aataagctta tcatatggat aatttgaatg
     3121 tcctattgcg aatatttcca tataaacacc ctcctattct ttgaaattag tatctatata
     3181 tgtagatacc cgatttactt aaaattaaat aaagtattac tttaaatacc caatatcttt
     3241 ttttcttgat attgtatat
//
LOCUS       DAYAOX010000299         3256 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.302, whole genome shotgun sequence.
ACCESSION   DAYAOX010000299 DAYAOX010000000
VERSION     DAYAOX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.302"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            323..1036
                     /locus_tag="ACGI57_003510"
     CDS             323..1036
                     /locus_tag="ACGI57_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9718556.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(1308..2846)
                     /locus_tag="ACGI57_003511"
     CDS             complement(1308..2846)
                     /locus_tag="ACGI57_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9718557.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         1124 a          447 c          464 g         1221 t
ORIGIN      
        1 agtttaaaag ggagttgaac ttaaagtttg attagaaaaa gtcaatttta agacaactcc
       61 ttttatttta ctattgtatt aatctgtatc tgtaagtgta ttagtctaat attataaact
      121 ttggatgaaa ttaattttct ataggtttta atgtatcata cactttattt taaagaaact
      181 tatcttaatt ttcatagtga ttgaatttgt ctattagaga tgttactatt agtaatgtaa
      241 aagcaagagt agtgaatata aaaaacaaga tagtgatact gcaagattag aatataataa
      301 atttttatgg agagatttca atatggatac atgttttaat aaaaaaatat tacttgttga
      361 tgatgaaaag gatatagtag atttaattga agaagtactc ataaatgatg gttttaaaaa
      421 cattattaaa gcttataatg gtctagatgc tatttcgctg tgtaagatag catgtccaga
      481 tgttgtcatt cttgatataa tgttaccaga tatagatggt atagaagtgt gtaaaaaaat
      541 tcgagagttt tcatactgtt ctattctttt tttatcatca aaaaatgatg atattgacaa
      601 aatactaggt cttagtagtg ggggagatga ctatataaca aagccattca gtccaagaga
      661 aattgctttt cgagttaaag cgcaattacg tagacaacag tatcaaagca ttgtaccatc
      721 agatagtgaa gttattaaaa taggagatat tactattgat atagaaggaa accgtgtata
      781 taaagataga aatgagattg agttaactgg aagggagtat catcttttaa gctacatggc
      841 tcaaaatgta aataaaatta taggaaaaga aagactctat gaacaagtat ggggagtata
      901 tagtagtatt tgtgacaata caattatggt gcatatacga catataagag aaaaaattga
      961 agataacccc tcaaatccta aaatacttat aacagttaaa ggactaggat ataaacttgt
     1021 gaatagaatc gattagaata gattttaata ttaatgaaga tatctcaagt tgaattttta
     1081 gttaatgagg cattctttta tgggtaaaat caaatatatt ttcgttatct caacaatgaa
     1141 gaagaggtct atctctattt tgagatagcc tcattaagtt tatatcctgg taatgtactt
     1201 tatcttctaa taaaattttt ttagtttatt tgcagtgaaa cattaactag aacattaaaa
     1261 tttgcattga aatactaatt aaataatatt gtatattgtg tataaactta tactaattgt
     1321 ttttctatat taaatacttt atcaatccaa cctttataaa atttttcttc atgggaaact
     1381 aggattatac ttcctctaaa ctgtttcaaa gcattttgta aagcatcctt tgtctctgca
     1441 tcaaagtgat tggttggttc atccaggata agaaaattac aaggagataa catcatacaa
     1501 cataatttaa ccttagattg ttcaccacca cttaaagtag atacactcct tgaaacatgc
     1561 tcttccttca caccacatct ggctagatga cgtctaatct cttttgtatt taattttgga
     1621 tatttatctg ctactatttg aagtggggtt ttatcaggat tttcccattt taaatcttgc
     1681 tcataataac caattttaac ttgttctgaa aactggaaat ctccagatat acgaggaata
     1741 tctttgacta atgtctttaa caaggtagat ttaccgattc cattaaaacc agttataact
     1801 attttttgac ctccatcaac agagaaattt aattttggaa gtaaagaata gtagtatcca
     1861 acttcaagat ttgttatagt aagagcattt tgagccgata tttcaatttc tgaaaattga
     1921 atatttggtt ttcctgtaaa actaggagga gctattcttt ctattttatc caattgtttt
     1981 tgtcttcctc ttgcaatttt tgaatttact ccagctttat ttttacgtat aaatgtttct
     2041 tctttttcta ttttcttttg ttgtgcttga taccttctaa tataatcttc tcttaaatgt
     2101 gatttttgtt taaggaactc tgaatatttt ccatgatatt tcttaatcat gccaaattct
     2161 atatcaagaa tacctgtaga aattttttct aaaaagtcaa aatcgtggga aactacaata
     2221 aatgccccat taaatgtatt taaataattt gagagccatt caacatgttc tttatctaaa
     2281 aagtttgttg gttcatctaa taatagaaca tcatggttag aaagtagtaa ttttgctaaa
     2341 attacctttg ccctttgacc accactaagc tctccaacaa cacgattcat tcctattgaa
     2401 tctaatccaa gaccatttgc tattttattt atttcattat ctatagaata gaaattactt
     2461 gctattagtt gttcctgata atctgatgct ttgattagat attgctcatt ttcagatata
     2521 gcactttttt gatagagtaa attcatttcc ttttctattt tataaagttc ttcaaatgca
     2581 gtgtgtaaat agagtgatat tgttgtatct ctattgattt ctgcatattg gtctaaatgg
     2641 cctatattga tattaggttg ccacttgatt tctcctgaat ctggaacaac ttctcccaat
     2701 aaaattttaa ttaatgtact tttacctgta ccattttggc caactacacc tatatgttca
     2761 cctttgaata aatcaaagtt agctttctca tacagtgatt tatccataaa acattgtgat
     2821 aaattagtta cttttaataa actcataatt caaactcctt ttcttttatt aaaaaattta
     2881 tggaaataaa aaaagcagag gaatggcatt gtaaaatgcc gacctctgct tttggaaaaa
     2941 caaaaggtca tagacaagca ttgttgtcta tgaccagaaa atctatatta tctatcggca
     3001 aataggtaat tttaaataag catagccaaa aaactatact aaacctatat ttgccgatga
     3061 agatagaaag ctttaagctt tcagattaac ctctacacaa cgcttcatcg tatacatttg
     3121 tgtagagtta agttttctaa caagtttaac taattctata taaatcataa ttattcacat
     3181 cctaatctta gtattaattc tagtttatct gtatttaaat tgtacttttg tactttgtgg
     3241 attatcttat ttattt
//
LOCUS       DAYAOX010000300         3235 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.303, whole genome shotgun sequence.
ACCESSION   DAYAOX010000300 DAYAOX010000000
VERSION     DAYAOX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.303"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            55..1182
                     /locus_tag="ACGI57_003512"
     CDS             55..1182
                     /locus_tag="ACGI57_003512"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFG9718558.1"
                     /translation="MKGKITMDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEG
                     KDVIAEAQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESK
                     DVSILAAYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQ
                     MLLMGFKNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLN
                     NIKQEVVETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLH
                     SDIAQSKRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIG
                     RTGRAGEEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1559..2743)
                     /locus_tag="ACGI57_003513"
     CDS             complement(1559..2743)
                     /locus_tag="ACGI57_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718559.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1235 a          464 c          419 g         1117 t
ORIGIN      
        1 aaaatactta caaatatgat attattaatt ggttaataaa aaagaaaatt tttcatgaaa
       61 ggtaagataa caatggattt caaaagctta gggattagtg aaaatacaat aaatattcta
      121 aaaaaatcag gaataacaac acctacacct atacaaaaag aaagtattaa actaataaaa
      181 gaaggaaaag atgtaatagc agaagctcaa actggaacag gaaaaacact agctttttta
      241 cttccgatat ttgaaaatat atcattagat ataaatgata tacaagtact tatactttct
      301 cctacaagag aactagctat tcaaataaca gaagaagcta tgaaacttaa agaatctaaa
      361 gatgtaagta ttcttgcagc atatggagga aaagatattg gttctcaaat aaagaaacta
      421 aaaggaaata ttcatatgat tatagctaca ccaggtaggc ttttagacca tctaaataga
      481 aaaacaatag accttagtaa gttaaagact tttgttttag atgaagcaga ccaaatgttg
      541 cttatgggat ttaaaaatga agtggaagct attttaaaag agacttcaaa caaaaagcaa
      601 acactatgct tttctgcaac tataaattct caagtaaaaa aattagctta tagatacaca
      661 aaaaatcctg ttgtagtttc tattcaaaaa gaagaaatca ctcttaataa tataaaacaa
      721 gaagtagtag aaactacaga tagaaaaaaa ttagatgcct tatgtaaggt actagatgaa
      781 gataatcctt ttatggcaat aatattttgt agaactaaga gaagggttga caatcttgaa
      841 gaagctcttg ctataagagg atataattgt caaaaacttc atagtgatat tgctcaatca
      901 aaacgagaaa gaataatgaa atcatttaga aatttagata tacaatactt aatagctaca
      961 gatgtagctt ctcgtggtct tgatatatct ggagtaagtc atatatataa ttacgattta
     1021 ccagaaacac cagaagacta catccaccgt attggtagaa ctggtagagc tggtgaagaa
     1081 ggctatacat gtgcttttat tgaccctaaa aatgaacgaa tgttaagtga aatagagacg
     1141 gctatagaat ctaaaattag tagaagaatt atagaattat aatttataga ttgcttaaaa
     1201 taccaatgcc acacaaacac tgtaatatat agaaagctac agaaaacatg tatcaatcta
     1261 tactttgctg ttatactatt ttatattaaa gaaaaaagag gctatccaaa gtaggattag
     1321 tctcttttat tgtcaaaata atgaaaatat atttgacttc atataaaaaa gagtgcctta
     1381 taaactaaaa gtccattttg agataccctc tttttatctt ttattcttag ttttatattg
     1441 cttgtttata gttgtttaat attagatttt gcaaataatc ttaataaaca tgcagatata
     1501 ttatctatag tattcttatt tatagtattc tctttagcaa tattttttgc tactacatct
     1561 aatttacatt ttcatttaat tccgctggcc atttagcttc tggattttcc tttgcaaact
     1621 tcttattctt ataatgtcct agtgtaagaa atggaattac tatagtgaat agaccaagat
     1681 aaccacaata tccatatcca tatttaacaa tattacttaa tcctgtaagt gatataagca
     1741 ttgacactac cattattgca caagctgtaa atacccttct tttcattctt gaagaaagga
     1801 aagaaagtgc tttatggtct tcaaatctat ttacaagacc aaatatagta gttactcctg
     1861 tagacattaa acaaaggaat aatgcaacat tataaattat gtatatagta tgatttccag
     1921 attgacctgc aacataaaga gtagggagtg ttgtttgccc agcagcaata atttctctat
     1981 accatcccat tagcatagtt acagcaagtc ctaatgcaat tgcattcata acaaatgaaa
     2041 ttatcattgc cttagtagct tcttttgagt tcttaagaag tggtcttgaa catcctgcaa
     2101 gtgtaggtat aacaaccgat tgaaaacctg cataaataaa agtattaagt attggcttga
     2161 tatagccagg tgtgaaactc gttctatttg ataatatttc agttatttct ggtactttag
     2221 cttttatacc aacaataaaa atagtaagag tacatactag aatagctatt gtcattgctg
     2281 tagctgcttt agatacaagc atagcaccaa atatagtaaa gacaagaagt acaactccaa
     2341 taataaatac agctacaaaa taatttactc ctatactttg aaatactgct gctgcacctg
     2401 ctataacagc acttacagct attaaaacca ttagatagta atatatctcc catattattt
     2461 caagttttgg atatggtctc caaagctcac aaaataaatc cttataattc ctacaattgt
     2521 tattattata cattatgata acttctctca tggtagcagt aagtataatc attgctaaaa
     2581 ttgccataag tggtgctgtc catccatact gcacatagta ctgagtttct tgattccctg
     2641 tagcaaatcc tcctcctgca tgtgaactaa aaagaacgct tgcaacacca aaaatagcta
     2701 acatagagcc cgttactttt gctttcttta cacttttttg catgaaatca tcctccctaa
     2761 aatgatattg aaagttttta ttttaacact ttattgataa aacaataaca tttttgctca
     2821 ttaaattcaa ctatattttt ccaatgactt tacattttca acttatttcc acctttatat
     2881 taaatatttt ttatatattt attttatttt ttaaatagta ttcaaattca ctttgttttt
     2941 taattttatg aaatatcttt tttaatgtta aatattatac attttttctt tattattata
     3001 taattttttc tttattatta tataataata atcgtatagt ataaaattcc ttcttttatt
     3061 acattcattg gaattatcaa actttaaaag aataaatcaa tatattttgt gaaaaaatat
     3121 actgtaatgt aagtaacttt gatttattta tatttattct aacattatta atacataata
     3181 aaattgttta ttaatgaaaa tatattctca ttaaaaataa atttttttac aatat
//
LOCUS       DAYAOX010000301         3203 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.304, whole genome shotgun sequence.
ACCESSION   DAYAOX010000301 DAYAOX010000000
VERSION     DAYAOX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.304"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(150..974)
                     /locus_tag="ACGI57_003514"
     CDS             complement(150..974)
                     /locus_tag="ACGI57_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HFG9718560.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNT
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(985..2388)
                     /locus_tag="ACGI57_003515"
     CDS             complement(985..2388)
                     /locus_tag="ACGI57_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9718561.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDDGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(2392..3117)
                     /locus_tag="ACGI57_003516"
     CDS             complement(2392..3117)
                     /locus_tag="ACGI57_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9718562.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
BASE COUNT         1087 a          535 c          275 g         1306 t
ORIGIN      
        1 aatattatat tctaatgtta ttgaaattac aataactgaa tttctgctca taaatttaaa
       61 tattctacaa atctcatcta aataacaatt ttaatagacc taaaacaatc ctcatactgt
      121 tcattttgta ttaaaaatat cacttacaat caaaaaccat ttattaagta tattcctaag
      181 atatacaata tatatacatg gattggatag tataaataaa ataaatactt catactcttc
      241 cctttttctc cattgtaaag taacattaaa ggtagagaag caatcatcat ccattggtaa
      301 ttttgtaaaa tagaattttt taaactataa tctccatttg acattgttgt aaatataaat
      361 atacacatta aaacataaca aatactcaaa ctgaactttt tacctctaca caaataaaaa
      421 ataatcccaa gtataatcca aattggtcca ccttctacta aaaatggtac tggtaccaaa
      481 atcataaata aagcaataac tctgttagtg actttaaaaa gtaaagctac tgtaaatatt
      541 cctaaaatta taggaatgat tatcatcaat aatcctataa taaaatattt aatttctttc
      601 tcttttctaa atttctttat gaactctatt gcttgtaaat aaatcgctat taaaaataat
      661 gttgaaaaag tgttatttat aattatagct ccttctggat gtgaaaatac attattaatt
      721 atttgatttc ctatagccat aataacagac caaatataca atctacttat atattttttt
      781 ctgttactag tgtgataaaa tccttcaaca gccatgaata taaacagtgg tgctgatatt
      841 ctgcctaccc aatgaaacca aattggtatt tgcattgttt gtggcataaa tgctgctata
      901 tggtcaaatg tcataaatat taatgctaaa atcttaatag taaacccact tatacctttt
      961 ttaaaaaaat ccatatcata tctcctaatt ttgttttatc tctataataa tttttgttcc
     1021 tattccaatt tcactactta cataaatttt acctttatga atctctatta tttctttaga
     1081 aatagccata ccaagacctg aacctttatg catctcacct gtattagtac ctctataata
     1141 ttttttaaaa atatgtttca aatcactttt gcttattcca attccatcat cttttattat
     1201 aatttctata ttatcttttt ttacaatttc tacacttatc aaagttcctt cgctattatg
     1261 aactattgaa ttaaatataa gattggttat agcccttctg aataagattg aatctacata
     1321 tacttcaatt aaatcttcat tactttcaaa ttcaatattc ctatttttat atctattatc
     1381 atttaaaata tcaattatta tatctctgac taaactgact aaatttattt ttttcttgtc
     1441 cagaactata gtattatttt ttaatcgagt agataaattt aaatcatcaa ctaaatcttt
     1501 tatatactta gacttattat atataatttc tgtatactct tgtatttcat cttcttcaaa
     1561 ctcataatct ttgtcattaa ttatctctgc atacccttgt atagatgcta gaggtgtctt
     1621 tatatcatgt gttatatttg ccaaccattc ctctctcaac tcttcatttt cttttctctc
     1681 aacttcattt actcttaaag tatcagctaa catatttata tttttaaaaa cttcttcata
     1741 tatacctctg tccttaagat ataaactata atttccatta gccatagtct caatactcca
     1801 tagtacgctt gaaattggtt ttgtaagttt tctactaaat aaatatccta cacctaaagc
     1861 taccacgcta tcaattacta gtgttataat gataacttta tttaaagttt ttttaatgtt
     1921 attttgagaa taggttaaaa ttattcttga tatatctcta ctaggtatac ctactattat
     1981 gtttagatgt tttttgttta aatatttttc taatacaaaa ttaacatact ttgtttctat
     2041 atacttgtaa ttattcacca tttgaaaggg tgtatatttt ttgggagtat cctttggcac
     2101 atttactcca taaacttcct ctcctaaatc atttaaaacc tgtatccagg aatttgattt
     2161 ttctaatttt tcagctccaa tcttagatag tttaaattct ccatcttttt catataaatc
     2221 tttttcaaaa gaacgtgcaa attcttcagg attatttcca gaatctacaa ctttaaaaaa
     2281 actattagtt gaaatcacat acaatataga tatgatattt ataattacta ctgatatagc
     2341 tacaaacaca attgtaaata taaaatttct agtaatcttc cacttcatat tctaatcttc
     2401 ctttactgct aatttatatc caagaccttt tattgtcaga atatattcag gctttgaagg
     2461 attatcctct aatttttccc taatttttct tatatgaacc attatagtat tgtcaaatcc
     2521 aacataatct tctccccaca ctttatccca tagtttttct ttacttataa tttgattttg
     2581 attatgtgca aataaagata ggatttttag ttcctttgca gacagtccta tagattttcc
     2641 atcttttata agttctgccc tactttcatt taatatatat gggccaaatg atataatttt
     2701 ttcttcatta ttatttagat ttttactttc gttttgagca tcactaaaat aacttaatct
     2761 tctcaaatgc gctttaactc taaaagccac ttccttaggg ctaaaaggtt ttgtaacata
     2821 atcatctcct ccaatcgcta gtccaagtaa cttatctaat tcttcggttt tagctgataa
     2881 aaatattatt ggagaattgg aggtttttct tatttcttta caaatatcat aaccttcgcc
     2941 atctggtaac ataatatcca atacaatcaa atcaggatta atacttttaa ataattctaa
     3001 tccttctttt ttagtttttg cagtatatat attattaagg ccctcttttt ttaggacagt
     3061 ttctaaaagc ttcaatattt ctacttcatc atctattatt aaaacacgat tttccataaa
     3121 caacctcctg taataattta catatatatt agcatatatt ttatctaaaa ttcatttatc
     3181 ttatttttaa ttaaactaaa ata
//
LOCUS       DAYAOX010000302         3201 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.305, whole genome shotgun sequence.
ACCESSION   DAYAOX010000302 DAYAOX010000000
VERSION     DAYAOX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.305"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..617
                     /locus_tag="ACGI57_003517"
     CDS             <1..617
                     /locus_tag="ACGI57_003517"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFG9718563.1"
                     /translation="KMINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFE
                     VRSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPV
                     KFIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYG
                     SIPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            915..1253
                     /locus_tag="ACGI57_003518"
     CDS             915..1253
                     /locus_tag="ACGI57_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HFG9718564.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1660..3096)
                     /gene="miaB"
                     /locus_tag="ACGI57_003519"
     CDS             complement(1660..3096)
                     /gene="miaB"
                     /locus_tag="ACGI57_003519"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFG9718565.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT         1101 a          471 c          365 g         1264 t
ORIGIN      
        1 taaaaatgat aaacgatatt aaaaataaat tcaaaaatta caaaccatat ataaatggat
       61 acaagaatat gaagagagca tctgttttaa ttccaatagt agaaataaat aacacacact
      121 atatattatt tgaagttcgc tcaaaaaatt tgaagcatca acctagcgaa atttcttttc
      181 caggtggtaa aatagaatca ggtgagactc catatgaagc tgttattaga gaaacttgtg
      241 aggaacttgg aacttttagt gacaatatag aagtaatatc aagtcttgac ctccttataa
      301 caccagtaaa gtttatcata catccatact taggatatat aaaaaatatt aataatttaa
      361 atataaacaa ggatgaagtt gaccatactt ttttggttcc tataaaatat cttttagaga
      421 atcctccaaa tacttatagt aatactgtaa aagtttttcc agatgaaaat ctaccatatg
      481 gttcaattcc aaaccaaaaa gattataaat ttgaaagtgg aagttatcct attctgtttt
      541 ataaatataa agattatgta atttggggaa tgacagcaaa aatactagaa aactttttaa
      601 attttttatc agaataacac tcataataaa ttatcaaaat ttttacataa gaaatttata
      661 aaagtttcta aatttaacaa ttctaaaata tcaataacta tattttgtag cactttttta
      721 ttttattatt gtttgaatat ttgatggttt tcaatgcaat ttcaacataa atactatata
      781 ttagtgtata ctgttgatga attaaagtat gataatctat ttaataaagt atgaagtaaa
      841 tgattttaat gtgcctttat ggtaaaatta ctaataagta ataaaattaa ttaaaatata
      901 tggaggtcct tataatgttt gctgtaggat ttgttgttat aattgcactt gcctttttca
      961 ttttaagtgt actattattt aatggaaaag ccagtatgct actagcaggt tataactcta
     1021 tgagtaagaa agaaaaagca cgatacgata aaaaaaaatt atgtagaagt tctagcattg
     1081 ttacttttat tgtctctata atgctattta ttatggcgtt tttgggatat agaatagaga
     1141 gtggtcaaat ggacgaaaaa aaaatggttc ccttttctat aatatttata atagtcatac
     1201 tttccagtgt attttttaat atcctctact ctaacaaaaa atgtaaaaaa taaatagttg
     1261 taagttgagc agttaattat aaaagattga ctgctttttc atgtatattg actactctta
     1321 ttaaatattt taacatttaa aaatctatgt aaaaataatg aatatagtac aaacttctaa
     1381 taatgatttt acctttgtgg ttacagtcca ctatatataa tttaatatga taacagggaa
     1441 ttattaatga caactaagta aacttttata aaatataata aaacaaaaat agatgtacaa
     1501 tgaaattata tcttatatca aatttatttt tattaatagc aaaaacttac attagcataa
     1561 catatgacaa attcgcttaa aataaaaatt tttaatataa tttgttctat agtacatcta
     1621 ttttgattag tttacataat tttattattt tgtacaatat taattattta ctaatatccc
     1681 atttaatgaa aaagttctag gttcagtaat tttgaccata actaatttac caattaaatc
     1741 atcatttcca ccttcgaaat ttacgagttt attttgtcta gttctaccag caaacttatt
     1801 ttcattattt ttacttcttc cttcaactag aacttcaact attctatctt tatatccatc
     1861 atttatttct gcagaaatcc tatttacagc ttctaacacc ctattaaatc tatcatgctt
     1921 tatatcttct ggtatttgat tttccatttt agcagcagga gttccctgtc ttttagaata
     1981 aataaatgta aaagctgaat catatctaac tttttcaacc acatctaaag tgtccaataa
     2041 atcttcttct gtttctcctg ggaaaccaat cattaagtct gtagaaaaag caatacctgg
     2101 aacttccttt ttagcttttt ctattatttc taaataatat tcctttgtat aatgtctatt
     2161 catttttttc aaaaggctac tgcttccgca ttgtattggt agatgtaaaa actcacacac
     2221 cttatcacaa tctctaattg cgtatattac ttcatcagat atatcttttg gatgagatgt
     2281 cataaatcga accctttcaa ttccttctat atcattcact tttcttaaaa gttctgagaa
     2341 tgaaactgga ttttcaagtg tcttaccata agaatctaca ttttgtccaa gtaaagtgat
     2401 ttctttcgtt ccatttgcaa caagctcttt tatctcgtta attatatctt caggagttct
     2461 acttctttct cttcctcttg tgtatgggac tatacaatat gtgcagaaat tattgcatcc
     2521 atacattata tttacaaatg cttttaattc aaattttcta ttacttctaa gaccttctat
     2581 tacttctcca tccacatccc atacatccac taacattttg tcagaattta ttgattctgt
     2641 aagtaattgc ggaaatttat atagattatg tgttccaaat ataaggtcta catgtttata
     2701 tttttttctt aattcctcta caacatgtgg ttgttgcatc atacatccac atacagctat
     2761 tttcatatct ggattttttc cttttagatg ttttaattgt cctaagttac catatacttt
     2821 tagctctgca ttttctctaa ctgcacaggt gttatatatt attaaatcac tttcttcaac
     2881 catcattgac atttggtatc ccatctcttc taacattgaa cataattttt ctgaatcatg
     2941 ttcattcatt tgacacccaa aggtttgagt aaacacgagt tttctctttc cagttctgtt
     3001 gaagtattct tcattttgtc tgtgtatttg ttctatatat ttatcttgtt cttgtatttt
     3061 ctctataggt attgttactt gttttctttt gctcacgtct attcctccta gttaatttct
     3121 tatattacac ataaccttaa ttatatcaaa taagaaaaat cttttaaaga tattattatt
     3181 tttctagttg tgttttaata g
//
LOCUS       DAYAOX010000303         3158 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.306, whole genome shotgun sequence.
ACCESSION   DAYAOX010000303 DAYAOX010000000
VERSION     DAYAOX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.306"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(3..524)
                     /locus_tag="ACGI57_003520"
     CDS             complement(3..524)
                     /locus_tag="ACGI57_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFG9718566.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
     gene            complement(576..1424)
                     /locus_tag="ACGI57_003521"
     CDS             complement(576..1424)
                     /locus_tag="ACGI57_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFG9718567.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            complement(1574..1855)
                     /locus_tag="ACGI57_003522"
     CDS             complement(1574..1855)
                     /locus_tag="ACGI57_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718568.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            2240..3094
                     /locus_tag="ACGI57_003523"
     CDS             2240..3094
                     /locus_tag="ACGI57_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9718569.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
BASE COUNT         1084 a          461 c          405 g         1208 t
ORIGIN      
        1 aatcaatctc taatatcatt tagcaacttc tcaaaccatt caatattaac taaatggtga
       61 gaacgagaat tttctagcat tctattaaaa taatgtgaat ttgttttgtc attttctatg
      121 gattcattta atagagaaat tcttacttga gtttgcttaa tttgcgtttc caataattct
      181 attaactttt catttttata atcatttgca aataatagtg ttgcgtaaaa tgatagattt
      241 atatactctg ttttagaacc atacttatac attaattttt caaattcctg gcatccaagt
      301 tcagtaatta tgtagttatg agtttctcta tttttattag attgtttatt ttcaactttt
      361 tcaataaact ttttttcttc aagttgctgt aaattataat aaaaagaacc tttagtgaaa
      421 tttactatgt acttgtaatg cttcttttcc attaatgcta ataactcata tccataagaa
      481 tttggatttt gttttaataa tcctaaaatt aataatggta tcaaaaaatc acctctttca
      541 atctatattt attagtatat taatgaatat tttatctaat tatcattaag tttaataagt
      601 ttagattcaa actcatcata tgatatcttt cctagtgcca agtccatact atttatatat
      661 attttttctg tttttgtata tgtcttggaa ttactcggta ttatcaactc actttcatta
      721 gctaatgctt ccataatatg ctttttctta tgaaaagctt caaaataacg taatccaaat
      781 tttctttgag atattgcatc aatatgaata ccatcaggat tagatgtaag acctgttgct
      841 gtaacaaagt aacaattatc ttgttcaaaa gcaaatttct gtaactcttg attgataaat
      901 acatactcag tacaactttt tccaaaacct acttttccta agaagtctcc caatccacca
      961 ataataattg gtatatctgg aatatttaat tcttttctaa gagtttcaat aatagaaagt
     1021 aattttttat aataaaattt atagttgcca ttattactgt cattttctcc ctgatgccat
     1081 aaaattcctg tcaattcact gctttgtatg gcaaatttag cttcacttat agcatgttta
     1141 aaaagtatac catctatatt ccattcatct aaggaactgc ctccctcagc acaaggaatt
     1201 agcccaattc tatcctcttg attttcacag caccatgcat ctgcaaatga tgcagcaaga
     1261 cttaccccag aaactggtct gtcataatta ataggttctg tcatcatttg ccatcttcca
     1321 ttacgtaaca tctgtattct ttcattataa attggagtca cttcacttat gaatcctctt
     1381 ccagccatat tggactgtcc taacattaaa aatgatttaa tcattattat tattatcctt
     1441 tcttaaattc ttattgtatt tatttttaat agtagtctaa ttagactatc tatgtttata
     1501 ttatagtcta attagactat aagtcaataa tattataaaa aatacttgtc ctactttact
     1561 tattaaagaa gtgctaatga ctagcaactt ctttgtttga attttttatt tgtaattgtc
     1621 tgtctaccat tattttttct attgctccat atattattgt atctttttct tgtttttctt
     1681 ctaatgtgaa ctttcttcct aagattgttt caggagatac tacatggcag attgtctttc
     1741 catgtagtat tgtcatagat attttatatc cattctcgtc tgttggaaca cctatatcgt
     1801 cgttgaggta tgttatagta gttgtaccat ctaagtcttt ttctatgttt ggcatttaaa
     1861 tcccctcctc tttatatttt atgtatctta aaaattgtcc tattctcaat cattatttat
     1921 aattgaataa ctaagtgttg cttgattaag ggtaaacttc aaaaatagtt aactataaat
     1981 aaaatgttat atataattaa atgtctaagc taaaatatta taaaaatttc ataataaatt
     2041 aaaactgtta tcttatataa aaattagata ctaatttgtg atatacttta atgtgaaaaa
     2101 caatactatt tgttcatttt tgtagttaat tacagacttt ctcatgttca ttctttatta
     2161 agctagttaa ttagtgcata gaaagagagg attaactatt ttaaaataga tattatataa
     2221 attacaagga gctgatttta tggaatggat tgaaagatta aacaaagcaa ttaactatat
     2281 tgaagaaaac ataacaaaag aaattgagta tgaacaagtg gcaaaaatag cttgttgttc
     2341 tacatatcat tttcaaagaa tgtttgctta tatggcagat gtaccacttt ctgaatatat
     2401 tcgtcgtaga cggatgtctt tagcagctgt agaattacaa aatgatgata aaaagataat
     2461 tgatgtggct ttaaaatatg gatactcttc tccaacagca ttcaatcgtg catttaaaag
     2521 tattcatggt gttgccccat cagttataaa aaaaagagaa ataacaactt taaaagcctt
     2581 tcctccaatc agttttaaaa ttagtattaa aggagccgaa gaaatgaatt atagaattga
     2641 aaagaaagaa gcatttcgta tagttggtgt atctcagcca cttcataatg aactagaaaa
     2701 gaattttgaa attgttcccc aaatgtggca aaaggttgct ttagatggaa cgcttcaaaa
     2761 attaatccct atgatggata gtcaacttca aggtgtatta ggtataagta tttgtaataa
     2821 ttcagaagaa tggaagtatt ttatatctgt ttcaagtaca aaatctatag ataatacatt
     2881 agaagaatac actgttccag cttttacttg ggcaatattt tctggagaag gacaatgtcc
     2941 acaagcaata caagaactag aaaagcgtat tgtaacagaa tggttaccta cttctggtta
     3001 tgaatatgat aatggtcctg atattgaact gtacttaaat ccagaccctc aaaatgctaa
     3061 atttgaagta tggatacctg tagtaaaaaa atagtttaaa attaaaaaat gaagaaagca
     3121 taaaattaat agtataaata tttacttaga ttacaagc
//
LOCUS       DAYAOX010000304         3156 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.307, whole genome shotgun sequence.
ACCESSION   DAYAOX010000304 DAYAOX010000000
VERSION     DAYAOX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.307"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(15..290)
                     /locus_tag="ACGI57_003524"
     CDS             complement(15..290)
                     /locus_tag="ACGI57_003524"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9718570.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(409..981)
                     /gene="mobB"
                     /locus_tag="ACGI57_003525"
     CDS             complement(409..981)
                     /gene="mobB"
                     /locus_tag="ACGI57_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HFG9718571.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            complement(965..2146)
                     /locus_tag="ACGI57_003526"
     CDS             complement(965..2146)
                     /locus_tag="ACGI57_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFG9718572.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVISDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            complement(2357..3091)
                     /locus_tag="ACGI57_003527"
     CDS             complement(2357..3091)
                     /locus_tag="ACGI57_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718573.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
BASE COUNT          977 a          556 c          263 g         1360 t
ORIGIN      
        1 ttataaatac aaaactactt caatgcattt aatatctttt tatctaatgc tgttgtattt
       61 atacctgtta tatatcctgt tgcctttatt gtaggtatat cataagttgt tggtagtata
      121 gtagtagata cgattaaatc agcaccttct tgttttgttg aacattcagc aattttacat
      181 tgctcaactt ctgcttgtat attattttct ttaaccaatc tttctactct gtcacaaact
      241 attgttgatg ttgcgattcc tgctccacag gctactaata tttttttcat atattgtcct
      301 cctacaattt ctttaatatt ttataactca aaataaaatt gataaatttc ttatatcttt
      361 atttatataa ataattatat cattttatat aagaagaaca tcttaaaatt attcttttaa
      421 tatgtttttt atttcaacat tgcttatact taaaatttca ttttttatac aagactctat
      481 gtactttgat atatttttat aatcatttaa attaaactgt tcaatattca atttatattc
      541 tatatcactg acaataccta tcaaatcaga cttattagat aaaatatcct catacttacc
      601 ttttctagtt acttccaatc ttttaaatct ataattttta tatccttcta tgataatcaa
      661 gtccatatct tcatataatt gaatcatttt atataatgga ttcttctctt ttagttcttc
      721 aatatatgct actcgtttat ctgatattat acagactttt ctcgccccag attttctatg
      781 tttataagta tcttttcctt cctcatccat atcaaaatca tgacaggtat gtttaataac
      841 tccaacacga tacccttttt tatttaattc ttttattatg ccttcaatca atgtagtttt
      901 acctactcct gaatatgaga caactgacaa tatattatac ataaaattgt gtcctttcat
      961 aaaactatct aataacaaca tccactaatt gattttcttt tattgtctca ttctttttaa
     1021 tcttaatcat acaattactt cttagagttg cagataatct agcttttgca tctttcttat
     1081 catttagctc tacatacaat ttaccttcac ttgtatatag ttttccactt acaaatctat
     1141 ctttatttga aagtttatta aattctcctt taaatatagc ttttatatga tgtgttccct
     1201 tcttatatgt taaatactca ataacaggaa ctatcacatt atcaaatacc actattgctg
     1261 catgtggatt accagaaaga ccaaataata gcttttctcc caatgtacct acataacaag
     1321 ctccaccagg tttaatgttt atcttccaaa ataattctgt tgcccctatt tttttgaata
     1381 catctggaat taaatcttta tctccaacag aaacaccacc tgtggtaatt aaaaaatcta
     1441 cactctgtaa catattcttt accttttttt ctaactccaa tatatcatca gaacaattgc
     1501 atattgtatc tgcttgtatt ccaagtttta ataatcttga atataaggta tatctattac
     1561 tatcgtatat tttaccattc tctaaagtct catttatatc ttgtagttct gtacctgtat
     1621 tcaaaattcc aacttttaaa tcatcataaa cagttacttt ggatattcca agacttgcta
     1681 atactccaat accatcatat cctatattct gatttttatt aataatcaat tgcccctttt
     1741 ttatatcctc acccttaaat atgtaattat cgtattcctt taattttata tttaaagtta
     1801 tcatatcact tataactgtt acatcttctt tttttattac acaatttgca ccctgtggta
     1861 tctttccacc agtcattatc ttcactgctt catttttatt taatacccta ttaaatacat
     1921 ctcctgcata caaagtccct ataaccttta aatttctatt ttcagaattt gcattatatg
     1981 cataaccatc atagggtgac ctatcaaatg gaggactatc aattgttgaa tatatatctt
     2041 ctgcaactac cttatttact gcatctataa gatttactat cttgaccctg tttattggta
     2101 aaacattttt ttcaataatt tttatggcat cttctaattc tatcatttta aatactcccc
     2161 aatatatttt ttatttaaga tatttctatg taatatatta tagattataa aagttggaaa
     2221 tacttttatc ttatatttaa ttataactaa tagttatcta taagtgcagt tcaaatttta
     2281 taaatctaat aaaaaaactg ttcccagcta gaaatttcct tctatcttga aacagcatca
     2341 tatgctttag aatattttat tctaataaag ttccttttat tatttttaca attttatcac
     2401 tttcaatagt ttttatagat tttattttgt tatccacaat acttattgta agtacctctt
     2461 tttttaatat tttgttatct tctttcaatc tataactctt aactacttta ctgccttcat
     2521 gattatatgc tgttaaatca taattcgcat ctcctacaat ttcatttatt ctggaatctt
     2581 caacaccaac atactctccc aggtcaatat gtctaaaatt ttctagtgat acagccgatt
     2641 tttcatggtc acttttttca atcactatac tagattcagc atcaatgact gatatacctt
     2701 gtgatgagaa agaattggtt tcaacattga caactttatt ttcgttaata tcaacatata
     2761 aagcacttcc atctagttca tcatttccag tatattttgg ataaactaat gtcatataac
     2821 ttgatgtatt tatatcttgc attgtatttg attcttttaa aactttaggt tttatataat
     2881 aaaatgtatt ataaggtata cccatattct ttataatgta cttataatct ttattcataa
     2941 tttcattaac atcattttga acttttgtca tagttttttg ctgtgttgct ttacttgcta
     3001 cttgattttt ctctttagta ttttctttat gacatccaac aagtataata cctaaaatag
     3061 ttacacaaac taatgacctt tttaacttca taaatcaatc ctcctttctt aaattttata
     3121 gtatttatct atatctcaat ttacagttat ctcttt
//
LOCUS       DAYAOX010000305         3101 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.309, whole genome shotgun sequence.
ACCESSION   DAYAOX010000305 DAYAOX010000000
VERSION     DAYAOX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.309"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..313
                     /locus_tag="ACGI57_003528"
     CDS             <1..313
                     /locus_tag="ACGI57_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718574.1"
                     /translation="NFVNSNILKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIG
                     EKRLIDEDLYLIAKSKEVFDFFNLDGTFNLTLSFDIQKNENLLDSLLELRKSIIINER
                     I"
     gene            350..2944
                     /gene="clpB"
                     /locus_tag="ACGI57_003529"
     CDS             350..2944
                     /gene="clpB"
                     /locus_tag="ACGI57_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFG9718575.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNNFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
BASE COUNT         1303 a          308 c          577 g          913 t
ORIGIN      
        1 caattttgta aattcaaaca tattaaaagt gtttaataat attgctattt atcaatataa
       61 tattttagaa aatctataca ctattgattt taaaaagagt tttatagata aatgtcagta
      121 tttattaata ggtgaaaaaa gacttataga tgaagacttg tatttaatag caaaatcaaa
      181 agaagtattc gattttttta atttagatgg aacatttaat ttaacattga gtttcgatat
      241 acaaaaaaac gagaatttac ttgatagtct tttagagctt agaaaatcaa taattataaa
      301 tgagagaatt taatttattt ggatataata ttaaaatgga gggatttata tggacgtaga
      361 aaaaatgact ttaagagttc aaaaaagttt aaatgaggct tataacgaag cagtaaaaaa
      421 tcataaccaa caagtagatg taattcatct tttctcagca cttattaatc aagaggatgg
      481 attgatacca aatataatcg aaaaaatgaa tatatcaatt gattctttga gaaatactgt
      541 aaactttgaa attgataagc ttccaaaggt atatggagaa ggtgctgact cacaaggagt
      601 atcagctact agaaaaataa atgaggtgct tataaaggca gagagtattt ctaaagaatt
      661 taaagattca tatataagtg tagagcatgt tatgcttgca atgatggaaa cagaatcaaa
      721 atcagctgtt ggtaaaatat taaaacaata taatataaat aagaataact ttttaaatat
      781 tttatcacaa gtgagaggta gtcaaagagt agaaacacaa gacccagaag gcacatatga
      841 tgcacttgct agatatggaa caaatttagt agatttagcc aagaaaaata aattagaccc
      901 tgtaattggg agagatgaag aaataagaag gattataaga attttatcaa gaagaacaaa
      961 aaataatcct gtattgattg gtgagccagg tgtaggtaaa actgcaattg tagaagggtt
     1021 agcagagaga atagtaagag gagatgtccc agaaggttta aaagataaga ttgtattctc
     1081 acttgatatg ggagctttaa ttgctggtgc aaaatataga ggtgaatttg aagaaagatt
     1141 aaaggcagtt ttaaaagaag ttcaaagttc tgatggaaaa attatactgt ttatagatga
     1201 gattcatact attgttggag ctggtaaaac agaaggttca atggatgcag gtaatcttat
     1261 taaacctatg cttgcaagag gtgaattgaa ctgtattggt gcaactactt ttgatgaata
     1321 tagaaagtat atagaaaaag ataaggctct tgaaagacgt tttcagcctg taattgcaga
     1381 agaacctact gttgaagata caatatctat acttagaggt ttaaaagaaa gatttgagat
     1441 acatcatgga gtaagaatac atgataatgc tattgtagca gcagcaaaac tttctcatag
     1501 atatatacaa gatagatttt taccagataa agcaattgat ttaattgatg aagctggagc
     1561 tatgattcgt agtgaaatag attcattgcc aacagaatta gatgttgtta gaagaaagtt
     1621 atttacatta gaaactgaaa gagaagcatt acttaaagaa aatgatgata aaagtaaatc
     1681 aagattggat gatattcaaa aagaaatagc agagttaaaa tctaaaaatg atgaaatgac
     1741 agcaaaatat gaaaaagaaa agagtcaaat attagatatt aaaaacttaa aggctcaatt
     1801 agacgaagct aaaggtaaag ctgaaaagta tgaaagagaa tatgatttca acaaagcagc
     1861 agaggttaaa tatggtgaaa tacctaagtt agaagaacaa ataaagcagt acgaagaaaa
     1921 tatgaatgat ggttctgaaa attctctttt aaaagaagaa gttactgaag aagaaatatc
     1981 tagcattgta tcaaagtgga caggtatacc agtaactaaa ctagttgaag gagaaagaga
     2041 aaaattactt aaactagaag atgaacttca taaaagagtt attggacaag atgaagcagt
     2101 tacagcagtt tcaaatgcag taatacgtgc aagagcagga ttaaaagatg aaagaaaacc
     2161 aattggttca tttattttct taggccctac aggtgttggt aaaactgaac ttgcaaaaac
     2221 tcttgctaga aatttattcg atagtgaaga taatataata agaatagata tgtctgaata
     2281 catggaaaaa catgctgtat caagactagt tggacctcct ccagggtacg tgggatatga
     2341 agagggtggt caacttacag aagcagttag acgtgctcct tattctgtaa tactatttga
     2401 tgagatagaa aaagcacatg aggatgtatt taacatgttc ttacagatat tagatgatgg
     2461 tagacttact gataataaag gtaagacagt agactttaaa aatactctta taataatgac
     2521 ttctaatata ggaagtaatt atctattaga agcaggagga aatataacag agactactaa
     2581 caatttagta atgaatgaaa tgaagcatag atttaaacct gagtttttaa acagagtaga
     2641 tgatattata atgtttaagc ctttagacca agaaaatatc aagaaaatta tagatatatt
     2701 tatgaaagat ttaaaaaata gattaaaaga aaaagatatt actattgaag ttacaaattc
     2761 agcaaaagat gttatggtga gagaaggata tgaccctgta tatggagcaa gacctcttaa
     2821 gagatatata ggaaatacat tagaaactat aattgcaaag aaacttattg ctggagatgt
     2881 gtataatgga tgtactatag ttatagatgg aaaagatgaa aatattgaag ttttagttaa
     2941 ataataaaag aatcagttga gcataatata ctaaatattt tgctcagctg atttttattt
     3001 ataaactata ggaaaagaaa tattttatca aattaataaa acatttttaa gtcaatatgt
     3061 ccaaattaaa tttaactcaa aggaagtatt ttagtaaatt t
//
LOCUS       DAYAOX010000306         3020 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.310, whole genome shotgun sequence.
ACCESSION   DAYAOX010000306 DAYAOX010000000
VERSION     DAYAOX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.310"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..992
                     /locus_tag="ACGI57_003530"
     CDS             <1..992
                     /locus_tag="ACGI57_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFG9718576.1"
                     /translation="MYQYKKLINYLDKSICMGALSSGSTSVPKVLYRTYESWAGFFPI
                     QNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVISSGLNCKSWIRTIKQYNV
                     TNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTARNLKKYMPKSEIILYYGAS
                     ELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNTEYAVYNATKPYSVNDIGY
                     YDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIEDAIVLPYSDTIRGNQIVL
                     FVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTSSEKIDRLALLKML"
     gene            1068..1943
                     /gene="dpsA"
                     /locus_tag="ACGI57_003531"
     CDS             1068..1943
                     /gene="dpsA"
                     /locus_tag="ACGI57_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HFG9718577.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            1946..2536
                     /locus_tag="ACGI57_003532"
     CDS             1946..2536
                     /locus_tag="ACGI57_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HFG9718578.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(2743..2943)
                     /locus_tag="ACGI57_003533"
     CDS             complement(2743..2943)
                     /locus_tag="ACGI57_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718579.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT         1155 a          384 c          469 g         1012 t
ORIGIN      
        1 atatgtatca atataagaag cttataaatt atttagataa aagtatttgt atgggagctt
       61 tatcttcagg ttctacaagt gttccaaagg tattatatag aacatatgag agttgggctg
      121 gattttttcc tatacaaaat ggtattttta atatctctgg ggaatcaatc ttgtttgtta
      181 atggaacact tagttttaca gggaatctaa attctataat gtcagtatta tatgaaggag
      241 gtagtattgt aataagttca ggtcttaatt gtaaatcatg gataaggaca ataaaacaat
      301 ataatgtaac taacatatac cttataccta caaaacttca acttttggtt aaacacttaa
      361 aagaaccagt actcaaagtt gtaagtattt ttacagggtc tcaattatta tttgaagata
      421 ctgcaagaaa tctaaaaaaa tatatgccaa agtctgaaat tattttatac tatggggcaa
      481 gtgaattaaa ttatattaca tatttatgct atgatgaact aattgaaaaa cctctaagtg
      541 taggcagacc atttccagga atagatatat atattagaga aggaaaaatt tttgtgaata
      601 cagaatatgc agtatataat gcaaccaaac cttattctgt caatgatatt ggatattatg
      661 ataatgatgg atacttaata tttgaaggaa gaagtgatga cattataaat attggaggat
      721 ttaaagttag ttctactaag attgaaaatg aagttaaaaa aattcctcaa attgaagatg
      781 caattgtatt gccgtactca gacactataa gaggaaatca aattgtatta tttgtaataa
      841 ctatagacaa aattacgaag aaagatttgt tgattaaaat gaaagataat ctaatgaaaa
      901 atgaaatccc aaagaaaatt atctttttaa attcatttcc atatacctct tctgaaaaga
      961 tagatagatt agcattatta aaaatgttat aagtataata tttagtaatt ttagtgaacc
     1021 aatgtattga taaaaacata ttgtatatag taaaggaggc ttacaatatg tcaaatcaat
     1081 acgatataac tgttattggg ggagacctgc gacaagtgta catggtaaaa aaactaatta
     1141 ataaagggta ttctgtagtc acttatggga tatcaaatga aattataaat ggaatcgttg
     1201 gaaaatcaac ttcactagct gaagcactat ccaaaagttc cattatttta tgtccaattc
     1261 catttacaaa gaatcaagtt aatattgaaa atacggatga ttgtcttgat gctactattg
     1321 ataacttgct aattaattta tctcctaatc aaatattatt tggaggtaac ataccaacta
     1381 aagtttgtaa attttgcgag caacactcta ttcagattta tgattttatg aaaatgaatg
     1441 atgttgctat tttaaatgca gttgctacag ctgaaggggc tatagcagaa gctattaaaa
     1501 gaagtataat caatatacat caaagtaact gtttagtatt aggttatgga agatgtgcac
     1561 aaatacttgc aaaaaaatta tacgctttgg atgcaaatgt atgtgttggc gcaagaaata
     1621 atgatgcaag aagtacggct aatgcatttg gatattccag tatttcttta agtcaacttg
     1681 atgaaaaact tttacaattt gattatattt ttaacaccat accagagtgt attttaacga
     1741 aagaaaggtt agaaaaagta tcacaagaag taactattat agatatagct tcttctcctg
     1801 gaggagtaga ttattctgtt gttaaagaag cagggattaa tgcgactctt tgccttgggc
     1861 ttcctggtaa atattcacca aagacatctg gtgaaattct tgttaacgct atagaaaata
     1921 ttataaatga aagaagggat tagaaatgag acttgaagga cttacaattg gagtaggatt
     1981 tactggttcg ttttgtactt atgataaaat atttatagag ttagagaatt tggttaaaga
     2041 aggagctaat gtacacacta tattttcaga tgtatcacaa aatatcgatt gtagatttgg
     2101 aaattctgaa gagtttatga aaaaagcata tgaacttaca ggaaataaac ctatagttac
     2161 gattgaagag gcagagccat ttggacctaa aggaattgct gatattataa taatagctcc
     2221 ttgtactgga aatactgctg caaaattagc aaatggtatt acagattcac cagtattaat
     2281 ggcagcaaaa ggtcatcttc gtaatgataa accactagta atatcaatat caacgaatga
     2341 tgctctaagc tttaatttca aaaatattgg cattctcctt aattctaaaa atatatattt
     2401 tgtacctttt ggtcaagata attgcaaagc aaagcctaat tctatgattg cacatactga
     2461 gctaataatt ccaactatag aacttgctct agagaacaaa cagctacagc ctgttataaa
     2521 aagtccacat caatagaatt ggttcttata atattatgta ttagatacaa tactagcaaa
     2581 gaggctgtct caaaatagag agaagcctct ttttcttgtt gaaatgatga aaatatattt
     2641 aatttcatac aaaaaaatgc ctcatggact aaaaagtcta ttttgagcta atctccgtaa
     2701 tttttgctat attaaaatta tttacttaat ccaattttta aattagaatt tatatcgttt
     2761 tgactcttca aatatcaatc taactgttgt tgatacaaaa gaagaggaca taattaaaac
     2821 aaatactgat agtcctctaa taaacaaatt atctacataa taagttacta ttccaatacc
     2881 tccaatcgtt aaaattacac ttataatcaa agaaaaaacg ctaattgttt ttaatttttc
     2941 catgcttatc ctccttctta taaacttcaa atttatctat ataatattaa atatatttta
     3001 tttcatatta tataaaaatc
//
LOCUS       DAYAOX010000307         2977 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.311, whole genome shotgun sequence.
ACCESSION   DAYAOX010000307 DAYAOX010000000
VERSION     DAYAOX010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.311"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(268..444)
                     /locus_tag="ACGI57_003534"
     CDS             complement(268..444)
                     /locus_tag="ACGI57_003534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718580.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(498..594)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1391..1792
                     /locus_tag="ACGI57_003535"
     CDS             1391..1792
                     /locus_tag="ACGI57_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HFG9718581.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            1855..2856
                     /gene="rsgA"
                     /locus_tag="ACGI57_003536"
     CDS             1855..2856
                     /gene="rsgA"
                     /locus_tag="ACGI57_003536"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFG9718582.1"
                     /translation="MEYHDLYLARIISQEKNFYQAVCVNGVLFAEVSGKLRFEATIQS
                     DFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRKAAGKVNEEQVVAANIDIVFLCM
                     SLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCDNVHRKLQVVNSIALGVDVVVTS
                     SIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINCLMGGEFLNTKEIRIDDDKGRHA
                     TTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKSFADIEAYFGKCHFRNCTHTSEP
                     GCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSEYLAKKEKKFKNISKINKKIRR"
BASE COUNT         1054 a          386 c          503 g         1034 t
ORIGIN      
        1 aatttataat tttgttaatc taatccttca aactaaaaaa tcatttatat ttacagaatt
       61 tcattttaag agatgtttat tttcacatag ataagagaat ttaagttctc atatttatta
      121 tttgatacaa atttacatct ttattatatg tattttaaat aaaaaataaa attatgaata
      181 ctagaaatct atcttaatta gctttctaac ataaggctat ttaaagtaga taaaactaaa
      241 aagggttctc tagaaaacca aatctattta attatactta cttactaata ccatcacttg
      301 atgcaaacaa gattctacca cattttctcc gtactctagt ataaaatcat ctagttgtgt
      361 atctataaaa tcacaataat catatgaact accaatataa gcagtcaaca tatgtgtaca
      421 tctatccatt ttatctctaa acatatatat tattatcccc ctaaaaaact ttcataaaaa
      481 caaaaagaat atctcttttt ggcaactggc tgacataact catagtttat tttataagcg
      541 ttagtcccta tagctttgcg tcactaaatt tctctagttt tgccgattta gttttattct
      601 acaattacaa taatacaata gaattgctta ttattcaata tacttatttg aaaattaaat
      661 aaatttacaa atatgaacgt tattttatat caaaaaaaat agactatgca tgtagaatat
      721 atggaaaaac ttgattgtag gttttgtatg aaaagacagt tgttattaat ttaagtgaga
      781 taaattgagc aacaggcata aaatgagaaa acaaataata gcagttaatt tttattatta
      841 atactaggtg aacattaaat ttcaaaatac cactagattt tatagaataa gaaaatgaga
      901 caaacataaa tatatgaata tattaaatta tacttgaaca tacttatatt ttatgataaa
      961 atttcaataa taattattag tgaaggagtg tcttgcctga tgtcagagtt atatataatg
     1021 gtaaccaaca aataaatagt tggagacgca tatgggttct gtttaatcag aatttttaag
     1081 tgtatttttg atacatttga ttgttgtgtc ttactctgcc tttatagata actaacatta
     1141 gacaacaaat aataatatta catattactt tgttaaattg tggtgtagtg ggctttaatc
     1201 tgctacactt ttattaattt tacacagtat tattttgaat ataattatgt tgaaatagaa
     1261 gatactaaat tttctagtcc acaggggttt tctgtttttg tgtaggtaaa tatgtatacc
     1321 acataaaaag gcagatataa atacctgtgg actttttttg tagaaaatac acgatacgga
     1381 ggaaataaaa atgaattata aaaatagaaa acgagcttat tatcaggaaa attcctgtat
     1441 ggataacttt atttgcaaaa attgtggttg ccttgtagtt cctgaaggag ctggtacaca
     1501 acatagaaat cattgtccac attgtttaca tagtcttcat gtagatatca ttcctggtga
     1561 ccgtgctgca gattgtgatg ctaatatgga acctattgga gtgtgggtga gaaaaaatgg
     1621 tgagtgggca attattcatc gttgtacacg ctgtggacat cttagttcta atcgtattgc
     1681 agctgatgat aattcagtga aattaatgtc aattgcatta aaaccactgg cacagccccc
     1741 tttcccacta gaaagaatca ttgaaaagtt ggaaaaggag gaaacagaat gaatggtatg
     1801 aaacaaatca atttacaaga ctatggtcta aatagaaatt ttattactca aagtatggaa
     1861 tatcatgatt tataccttgc tcggattatc tcgcaggaaa agaactttta tcaagctgta
     1921 tgtgtaaatg gtgtactgtt tgcagaggtt tcaggaaagc tgcgttttga ggctactata
     1981 caatctgatt ttcccacagt tggtgatttc gttatgttgg atcgttgcaa taataaaaat
     2041 ggtaatgcta ttattcatca tgtgcttcat agaaaaagct tgtttgtgcg taaggctgct
     2101 ggaaaggtaa atgaggagca ggtggttgca gctaatattg acattgtttt tttgtgcatg
     2161 tcactgaata aggattttaa cattcataga ttggagaggt atctgtcttt aacatgggaa
     2221 agtggagcta tcccagtagt agtacttact aaatcagact tatgtgacaa tgtgcatcgt
     2281 aaattacaag tagttaattc tatagccttg ggtgtagatg tagttgttac ctcatctata
     2341 gagccagatg gttataaaca gatttctaag tatattaaac caggctgtac tgttgcctta
     2401 cttggttctt caggagttgg aaaatcttca ttaattaatt gtctgatggg tggagaattt
     2461 ttaaatacaa aggaaattcg catagatgat gataaaggaa gacatgccac gactagacga
     2521 gagctatttt taatttcagg tggtggtatt gttattgaca caccaggtat gagagagttg
     2581 ggaatgtggg atgtttcaga aggtttagat aaaagttttg cagatataga agcttatttc
     2641 ggaaaatgtc attttcgtaa ttgtactcac acctcagaac caggctgtgc tatctgggaa
     2701 gctattgctc atcaagaact ttcgcctaaa agatgggaat cctatcgtag gcttaaaact
     2761 gaagcagtat ttgcagacaa taaaagcgaa tacttagcaa aaaaagaaaa gaaatttaaa
     2821 aatatctcaa aaataaataa gaagattaga agataagtgt agtttttttg ttcattctat
     2881 gagtctatat actattaata tattaaattt gccatcaatt tgttacttta atgtgatttt
     2941 gatggcaaat ttggtttgtg gtattattta aagcaat
//
LOCUS       DAYAOX010000308         2891 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.312, whole genome shotgun sequence.
ACCESSION   DAYAOX010000308 DAYAOX010000000
VERSION     DAYAOX010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.312"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     regulatory      <1..60
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            143..628
                     /locus_tag="ACGI57_003537"
     CDS             143..628
                     /locus_tag="ACGI57_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFG9718583.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     gene            984..1382
                     /locus_tag="ACGI57_003538"
     CDS             984..1382
                     /locus_tag="ACGI57_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718584.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    1818..2081
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2232..2783
                     /locus_tag="ACGI57_003539"
     CDS             2232..2783
                     /locus_tag="ACGI57_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HFG9718585.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT         1246 a          261 c          432 g          952 t
ORIGIN      
        1 cgcaaaacta tagggactaa aatctttatg taataaaggt catgtcagcc agttgccaaa
       61 aagataataa ttctttttgt taaaagtatt tttagagtta tatattttaa taacttaata
      121 tttggcaaga ggagggctta agatgatatc tgaattaatg ttaaaaaatt ttcgcaaatc
      181 tagcatcaat tttaagacta aaattaaatt gaatttaaga gaaaaaccag acataaatag
      241 tttgaaatta aaaagtatac cagaaggtaa aattgttaag ttaaagtgtg tagatggaat
      301 atgggcagaa gttgagagta attatgacaa aggttggtta ctttataaat atttagaaag
      361 attaagtaat gctaaaaaca atagtaatga tacaataaaa aatagaaaaa agaattacat
      421 aggaaatata agaacaaatg gattaagttt agaacttaga aatgatagaa cattagaatc
      481 taaagttata actacaatcc ctgatggttt taaagttgaa gtatgttata gcgttggaaa
      541 atgggcaaga gttaatatca ataaaaatga aaaacgatat tctggatatg tatataatca
      601 atacatagaa gagcatttaa gtttataata cgatactagc aagctttaaa tatattaata
      661 aatcgcaaaa aagaatccca ttaatgggat tcttttttgt agaaacatta taacagaact
      721 aaagagaata aattaactaa tttattttta ttataaagta attataaatg gtaaatttaa
      781 ttgaaagtag atttaactta tactttatac accaagtatc ataaattaat actactttga
      841 aaaatcttaa aaaaagtaaa agttatgata ataaaaataa tattaaagat aataagatat
      901 ataagaaact tgaaaaaatt tgtaattttt agttatatat atattataat gaactagtta
      961 aaaattttta tgagggggaa aatatgaata agaagaaaaa attgattggg atagctatat
     1021 cttttctact tataatcagc attataaatc cattacaagt agaagcagaa gctattattg
     1081 agtctagtgt atacatttct tcaataaaag agaattatac atatgataat actaaattta
     1141 aaactattaa atctaaaagt agacataaaa gtaggaaata taataaaagt aaagtaaagt
     1201 tgaaaaaacg aagtataaga actaaaaagt atggatatca aactataaat aaatataaaa
     1261 gacaaaacgc aagaagacat aatatcctaa caaaattaat attgtccata atagtcatat
     1321 ttttggttat agttttagta gtgctaatat ttattaaaag aaagaagaaa aaattttact
     1381 aaaaataatt aaaagaatat cattttttta atcattggta tctatttaat aatatgatgt
     1441 aattaaaatg gagtaatatt ctgtttcaaa ggaggcatat aaatacagga atgagtatta
     1501 aatgatggtt aattaagtaa atttattgat aaatgtactt aattaacttt gaagaattta
     1561 attcctcatg aatcaaaata taaaattaca tcaacagtta ttcacaaaat taatataaga
     1621 agaattagag ccaaagagca gactattaga ccattaaagt aattattcat agaattttgt
     1681 atatgaaaag aaatgtatag tataatttac tttttttata aaaaatagat tacaaaatat
     1741 attttttata aaaaggtatt gacatttttt agaaaaaaga atatcattat tttaacaaca
     1801 aaattcaata caataaatta aatcctatga caaggaagag tagttaaatt taggttcagt
     1861 atagagagct gaggatggtg gaatcttagc aagaaaaaat ttgatgaatg gcccttagag
     1921 gaataaattg aaatcatagt tttgagagta gactttatcg tcagtcgcac gttatagcga
     1981 ataaggtatg taagtacctg ataatgagat gtatgaaaat tgaattttat attaattttt
     2041 atataattta ggtggcaacg cggataatct ccgtcctatt agtttaggac ggagtttttt
     2101 attttactga aaatttaaaa gatagaaggt ggtgagaaag atgttatgaa tttcaaaagc
     2161 taaagatgat gatatagtag gttggcaaaa aaataaattg aaaattaaaa aaataggagg
     2221 aagtacaaaa aatgaaaaca aatacaaaaa aattaacttt aaatgcaata cttttagcga
     2281 tgggattatt aatacatcaa atcacaccag ctataggatt accaatgaag ccagatgtac
     2341 cactagcaat gttatttgta atattggttt taaatagaga tgattataaa acttgtttga
     2401 tagcgggtat agtaacagga atatttacag cacttacatc aagttttccg ggaggtcaaa
     2461 ttcctaatgt aatagataaa actcttacag caaatatagt atttttatta atgaatatta
     2521 gttataatat gccttttata aaaaaattag ctaagaaaac acaagatact gtagtagttg
     2581 caataataat gccaatagga actcttgtaa gtggaactat ctttttatta gcagctcaag
     2641 ctatagttgg tcttccaggg gcttcattta cagctttatt cttagcagta gtattaccag
     2701 ctgttttaat aaatctagtt gcaggaatat ttttattcaa gattgttagt ctatcaataa
     2761 gaagagttag ttatcaagct taaacacgaa ataatatttt actttataaa gcattataat
     2821 aactaaataa cataagaata aagtagatta taactatttt attcttatgt tgtttaaaga
     2881 tttggtatta t
//
LOCUS       DAYAOX010000309         2888 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.313, whole genome shotgun sequence.
ACCESSION   DAYAOX010000309 DAYAOX010000000
VERSION     DAYAOX010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.313"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            203..1447
                     /locus_tag="ACGI57_003540"
     CDS             203..1447
                     /locus_tag="ACGI57_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718586.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDGLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            1644..2513
                     /locus_tag="ACGI57_003541"
     CDS             1644..2513
                     /locus_tag="ACGI57_003541"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HFG9718587.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECI
                     VLVTETGVEILTE"
     gene            complement(2567..2755)
                     /locus_tag="ACGI57_003542"
     CDS             complement(2567..2755)
                     /locus_tag="ACGI57_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFG9718588.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
BASE COUNT         1193 a          343 c          404 g          948 t
ORIGIN      
        1 gaattaatta taatatatgt ataaaacata ctctacatga atctaatgct aaattttgac
       61 ataataataa catatttatt tctataataa aaaattcaca aattataaaa aataaaattt
      121 tcaaaaaaat tataattttc aaaaaaacaa attgacacat ataaaaaaat tatgataccc
      181 taataaaaag ggggcaatga ttatggaaaa caatttaaat acagctaaaa gaaataatac
      241 tgaattatta caaaatgact cacttaaggt caacttacag ataggtgtag actttacact
      301 tttatcaaaa tcacttaaag ccttttataa aaaagaaaaa gatttttatt gcattgctct
      361 tgcgccatgc aatgtatcta gtgaaaaagc aaatagaaaa ataagtattt acgatatgat
      421 aaaagaaatt aatattttaa ttgaaagtat aactgataaa aatgaacttt taagtgaaga
      481 actctttact aataatttat cagaatttta tgacgaatct ttagatgaga tttatataga
      541 cttaaaacaa gcgtatttat atattaaaaa gaatacagat ggtcttaaca tagaagactc
      601 aattgaatat gtatttgatg tagatataag caatgatata aaagcaaaag aaaatccact
      661 aattaatttt aattcacttt ctttagtagt atggagtggc gaaacaaaac atattttaca
      721 acaaatgaat attttagatt tagaggatat tcactcttat tctatagaaa ataatacaaa
      781 tataactatc tatgaaaata atactcttca atataagttt gatgaagaat ctgaaaatat
      841 aaaagacagt agtatagata taattgaaca aatgcttata cttcctgatg acttgaatta
      901 tgataaagat gaagttgaaa atatgaaaaa cagacttgca aaaataaata ttaaatattt
      961 acagacactt aaagaaaagg atataaaaat aaaattaata aattcaaatc taactgatga
     1021 accagaattt agtgatttaa aatatcaatt acctccatgt tgggtaagat caggcaagac
     1081 ttggaaagat gtacctggta tatatagaaa taattctata gtggctaaaa taggttatag
     1141 taaccctagc tatgcaaatg tacattcatc aaaaaattta gaattacatg aaactgcaca
     1201 tgctatagat aaaaatgttt taaataaaaa atcaaattct gaagaattta tggaagtatt
     1261 tgctcaggaa agatataagc tttatgaccc taagcaagtt gcacatgcat atataagcaa
     1321 gtttatagaa gaattttttg cagaatcatt tgtacattat tatcttgatg aagattcaaa
     1381 aaatacttta aaggaaaatt gtcctctaac atatgatttt cttgaaaaac ttgaactaaa
     1441 ctattaagtg cgctaatatc caataataca tttaactatc ctcttttcat caacaataac
     1501 catataataa tttatacttt gaataaatca ctatctataa aggatttctt agtatttatg
     1561 ttataatatt tataatggtt atgttttaaa atagtttatg taacccataa tttaaataaa
     1621 taataaaaga aggatgaaaa gttatgaagt taaatagaaa tgatagctgt tggtgtggaa
     1681 gtactaaaaa atataaaaaa tgtcatatgg atatggataa taaaatagat tcatttgcta
     1741 agttaggtca tattgtacca actcatgata ttataaaaaa tgaaagtcaa attgaggaaa
     1801 taaaaaaaag tgctgtaata aacacaaaaa ttttggatta tatttcagat aaaatcgaaa
     1861 ttggaatgtc tacagaagat ataaataagt tggttcatga ctttacacta caaaatggag
     1921 ctatccctgc tccactagac tataatggat ttcctaaaag tgtatgtaca tcagtaaata
     1981 atgaagtttg tcatggtatt ccaagtaaag acattatctt aaaagatgga gatattataa
     2041 atgtagatgt ttctactatt tttaatggat actattcaga tgcttcaaga atgtttatga
     2101 taggtaatgt taatgaaaat gcaaaaaaat tagttcaagt tacaaaggaa tgtgtccaaa
     2161 aaggtcttga agttgccaaa ccatggggat ttttaggaga tataggtgat gctatcaact
     2221 cttatgcaaa gaaaaatgga tactctgttg ttagagaaat aggtggtcat ggagttggat
     2281 taagctttca tgaagaccct tttgtaagtt atgtatctaa aaaagggact gagatgctat
     2341 tggttcctgg gatgatattc acaatagaac ctatggtaaa tgaaggaact cacgaaattt
     2401 ttgttgatga agaaaataat tggactattt atacaaaaga tggtaaatta tctgctcaat
     2461 gggaatgtat agttttagtt acagaaacag gtgttgaaat actaactgaa taaaagacaa
     2521 atgcctataa aaattgaaaa ttcaattttt ataggcattt attttattaa tgcacatcta
     2581 ttgcatcaac tggacagctt tctctagctt ctgctgctga ttcttcagca tcacttggta
     2641 cttcatcaac tataacatgt gatttcccat catccttcat atcaaatact tctggacata
     2701 ttgatggaca taatccacaa cctatacaag tatcttgatt aacatttgct ttcataataa
     2761 aatcctccta gacgctacaa atttaaaatc ttgtttatca aagattgatg aaccaaaact
     2821 gtaacattca gttctttttt attatgtcct caaatttgaa aaaaatacct tttaaaataa
     2881 tttttata
//
LOCUS       DAYAOX010000310         2884 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.314, whole genome shotgun sequence.
ACCESSION   DAYAOX010000310 DAYAOX010000000
VERSION     DAYAOX010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.314"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            474..1511
                     /locus_tag="ACGI57_003543"
     CDS             474..1511
                     /locus_tag="ACGI57_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718589.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKSEW
                     SYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYDEY
                     FKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTKVG
                     RNFIRYSLRSI"
     gene            1622..2764
                     /locus_tag="ACGI57_003544"
     CDS             1622..2764
                     /locus_tag="ACGI57_003544"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9718590.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         1293 a          234 c          497 g          860 t
ORIGIN      
        1 gtttcaaggt ctatatgatt aattaaatcc atttttgtta tttataatta ttattgaata
       61 tatttttaca tataagatat aaggtgatag atagaaattg ttggattatg taaataatta
      121 atatataata ccattatggg caataaatac aaatcaaatg ataatatagt ctatttatgt
      181 aaatgttata ttgtatccta agtataatag taagtttttt gatagtggtg tagaaagtaa
      241 aaatctacta gcctaaattg atgtatggat tagtttagga acagtaattg catatcttaa
      301 gtttgatgtc atgttgggct actagaaata ggtcatcttt ttcacttgaa tatcttttct
      361 ttaaacatgg ggagtatcat atcaaaaaca tatgaaaatt aagtgattta tgagaacagt
      421 aatattttat atgatagtat agaatagtaa taaagtatta ggaggaacat tgagtgttaa
      481 aaaagtggtt tggtattgtg aaaaaaagac aaaaaagtga gtcagtaaaa gaagaaggtg
      541 aagtaatatt aaaaaacgaa aaaatattat ctgaagaaaa gttgatagat gaagaaggag
      601 ttgtagttaa tattgataat caaatattaa ctaaagtagt aaatgatgac aatgaaatag
      661 aagaaaaaat aatagaagaa gatttgttaa ttagtgaaaa taccataaaa ttagatgata
      721 aagaagcaat tattaatgat aaaaacatag aattatgtaa aaaagaagtt caagttgaaa
      781 gtgaaaaaat aggtttaaat aagtttgaag gactggacca aaatgaaaat tataatctag
      841 aaaaaaatgt tattgaagaa aaaaaagtaa gtgaatgttt gacagaagaa gatttagagt
      901 acataaaaga aattaaaata aaaagaggaa aaagtataaa agctataaac ttgtatacta
      961 aagaagagtg ggtttttgac actcatatac agtgtagtaa aaaactcaaa gttccattag
     1021 ggtacataag agaaaattta aaatatggat atatggatta ctttggagat gcaataaatt
     1081 atttaagtga agtattaaat atagatgaat actgcaaaag tgaatggagc tatctcgata
     1141 atagcaaatc tccatctgag atatttaata ttctaaacaa taaaatattt agtataaggc
     1201 tttctaatga aaaaagaaat gaaattttga caaatgataa aattgaagca ttaaaaatga
     1261 attatagatt tgaatgtatt gatgaagaat atgatgaata ttttaaaaaa tataagtcta
     1321 taatcaaaag aggtggaaag aaaaaagttg aattagtaaa taaaaaaggt gacattttag
     1381 aaatatttaa gtctttagaa gaatgtgcca tttatttgca aaaggaaaag aatgaagtta
     1441 tacaaatgtt aaaatatgga gatacaaaag taggaagaaa ctttataagg tatagtttga
     1501 gaagtattta atttcatttt aaattcataa gagtttcatt tctgattcac acatttaata
     1561 ttattttaat gtaaaataat aaaggtgaag tattgcacaa ttaaaattaa ggagggtatt
     1621 aatgtctgaa gaaaaaaatc caaaagaaaa tacattagat gaatctaaaa attcagataa
     1681 tgtaaattct aaaaaagatg atgtgtcaga tgaaaaaaat actgataaca aagttaaaaa
     1741 aagggaaata aagctaaaca acaaaacact taaagctatt gtagcaattg ttgcagtagg
     1801 agctatatgt ttaggtggag gctatgtttt tggcaaagaa gctggaagaa aattgcctgc
     1861 tacttcaaaa cactataata gcagtgaggt agtagctact attggggata aaaaaataac
     1921 aggtgaaatg ttaagaaaga gaatggagcc attattttat ttaaatgcaa aaactactat
     1981 gactgatgac gaaatacgtt catatgaatc aaatatgatt gaaactatga ttacatctga
     2041 tatattatcc tctgaagcag caaaagagaa aataagtata actaatgacc aactagatgc
     2101 tcagtataat aatttaatga gtagtataga atcagcaatg ggaatgacta aagaagagtt
     2161 tttaaaacag tttgatttga ctgaaaaata tataaaagat gatatgaaaa aagagctagt
     2221 agcatcaaaa tatttaaatg aaaaatcaaa agttactgat aaagaagcag agaactatta
     2281 caataaaaac aagagcaatt atttacaagt tagagcttca catattttaa taaaaactgt
     2341 tgatgataaa ggtaagcaag ttagtagtag caaaaaagct gaacttaaga aagaagctga
     2401 agaaatactt aaaaaagccc aagctgggga agattttgct actctagcta aaaaatactc
     2461 tgaagatagt agtgcagaat caggtggaga tttaggtttc tttggtaaag gtcagatggt
     2521 ggaatctttt gaaaaagcag cctttgcatt gaagaaggga gaggtatcta acaaacttgt
     2581 agaaagtgat tatggatatc atataattaa gaaaactgat gaaaaatatc agccatttga
     2641 agaggtaaaa tcagatttag taagcagttt aacatcagaa aaacaaaacc tacttataca
     2701 aaatctaaaa gaaaaatata atgttaaaat tacagataaa tatcaaatga aagctaaaga
     2761 atagataaag tcaatttatt aaaaaaaaga aaaattaact gtatggataa aagctatttt
     2821 tactctcaat atagagagta taaaatagct tttattattt tgcaataaaa tctatttata
     2881 tata
//
LOCUS       DAYAOX010000311         2878 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.315, whole genome shotgun sequence.
ACCESSION   DAYAOX010000311 DAYAOX010000000
VERSION     DAYAOX010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.315"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            79..1365
                     /gene="grdG"
                     /locus_tag="ACGI57_003545"
     CDS             79..1365
                     /gene="grdG"
                     /locus_tag="ACGI57_003545"
                     /EC_number="1.21.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     alpha"
                     /protein_id="HFG9718591.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            1451..2758
                     /gene="grdF"
                     /locus_tag="ACGI57_003546"
     CDS             1451..2758
                     /gene="grdF"
                     /locus_tag="ACGI57_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_082007859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2498..2500,aa:Sec)
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     beta"
                     /protein_id="HFG9718592.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
BASE COUNT         1100 a          319 c          614 g          845 t
ORIGIN      
        1 catagaaaat gctttatata ttaataaacg taataaatac ttttaattgc gattaaatat
       61 attaaggggg attttcatat gaagctagag ctagggaata tatttataaa aaatgttgaa
      121 tttggagaaa agacagaagt aaaagatgga gtactatata ttagtagcga agaaatagaa
      181 aagatagtat tagaagatga aaggattgta agtgtaaatg ttgaacttgc aagacctggc
      241 gaatcaataa gaatagctcc agttaaagat gtaatagagc ctagagttaa aattggagat
      301 gaaagcaaaa tatttcctgg aattataaat aaagtgaaga ctgtaggaag tggaagaacc
      361 catgtacttt taggagcttg tgttgtaact tgtggaaata tagttggatt tcaagaaggc
      421 gtaattgata tgagtggtcc tactgctaaa tatacacctt tttcaaaaac aaataatata
      481 tgtattgtta taaaagcaaa ggatggtata gatactcatg attatgaaga agcagctaga
      541 attgcaggat taaaaatagg tgcatatgta ggtgaggcag gaagagaagt tgagccagat
      601 gaagtcattg tatatgagac aaagccatta ttaaaacaag taaaagaata tccagacctg
      661 ccaacagtag catatgttca catgttacaa tcacaaggac ttttacatga cacttattac
      721 tatggggtgg atgctaaaca gatagtgcct acatttatgt atccaactga aataatggat
      781 ggagctataa tatctggaaa ctgtgtagca ccatgtgata aggttacaac attccatcat
      841 ttaaataatc cagttataca tgacttatat aaaagacatg gaaaagattt aaactttata
      901 ggagttattt tgactaatga gaacgtattc ttagtcgata aagagagaag ctctgatatg
      961 gtagctaaat taatagaatt tttaggagtt gatggagttc tagtgacaga agaaggttat
     1021 gggaatccag atactgacct aatgatgaat tgtagaaaat gtagtgaagt tggagcaaat
     1081 gtagttttga taacggatga atttcctgga aaagatggca agagtcaatc tatagcagac
     1141 gctactaagg aagcagatgc agttgtttct tgtggtcaag gaaatctagt ggttcatttc
     1201 ccagcaatgg aaaaaattat aggaacttta gattatgttg aaatgatgat aggtgggtat
     1261 aaaggatgct taaatgaaga cggaagcatg gatgcagagc tacaaattat aatagcatca
     1321 actatagcaa atggctataa tcatttgaca gcaagatatt attaaataag tatttgaata
     1381 taaagttctg ttgatgtaat cattggtcta actgatgtaa taactataaa gtgatttgga
     1441 ggattaagag atgagtaaaa tcagagtggt tcattatata aacaatttct ttgctggtat
     1501 aggtggagag gaaaaagcag atattccgcc agaaaaaagg gctggtgtag taggacctgg
     1561 aatagcattt cagagtcaat ttaatgatga tgcagagata atctcaactg taatttgcgg
     1621 agatacgtat tttggtgaaa atattgagag tgcaactcaa gaacttttaa aaatgataaa
     1681 agaagaaagc cctgatttat ttattgcagg cccagcattt aatgcaggta gatatggagt
     1741 agcttgtggg actatttgta aggcagttga agaagagtta aatattcctg taattacagg
     1801 catgtataaa gaaaatccag gtgtagatat gtttaaatta gatttgcata tagtgtctac
     1861 aggaaattca gcagcaagtc ttagaaaggt agttccaata atgacgaaac taggtcttaa
     1921 attggttaga ggtgaggaaa taggacctcc tgaagaggaa ggatatataa tgagaggaat
     1981 aagaaaaaat ttcttccatg aacttagagg gtctgaaaga gcaattgata tgttggttaa
     2041 aaagatgaaa ggggaaagct ttgaaactga gtatccaatg cctgaatttg atagagttac
     2101 acctatgaaa cctgttaaag atttatctaa gataaaactt gccctagtaa cttctggagg
     2161 gatagttcca attgataatc cagacaaaat tgaatcttca agtgctacta aatatggaat
     2221 ttacgattta acagatatgg attctatgtc aaataaagaa tttacaacta tacatggggg
     2281 gtatgacaga gcatatgtcc ttgaaaatcc aaatttagtt gtaccactag atgtagttag
     2341 agagcttgaa aaagaaggtg taattggaga attagtagat tattttataa ctactacagg
     2401 aacaggaacg agtgttggca attctaagag atttggagaa gagttttcta agaaattgtt
     2461 agaagatgat gtagatgctg taattttaac ttctacatga ggtacttgta ctcgttgtgg
     2521 agcaacgatg gtaaaagaga ttgagagagc tggtatagca gttgttcata tatgtacagt
     2581 agtaccaata tcacttacaa taggagctaa tagaattgtt ccagcagtag ggataccata
     2641 tccacttgga aatcctatgt taggagaaga ggaaagtaaa aaaattagaa agaatatagt
     2701 tttaaaagct ttaaatgcac taacagaaga tatagaagaa caaacagtat ttgaataata
     2761 aaaatacaac acaacactga aataaaaaaa gatatcaaaa aataattttt taaaattatt
     2821 ttacgatatc tttttttatt aggtaaattc gaaatttgca tttttttaaa ttaaattt
//
LOCUS       DAYAOX010000312         2854 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.316, whole genome shotgun sequence.
ACCESSION   DAYAOX010000312 DAYAOX010000000
VERSION     DAYAOX010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.316"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(30..1706)
                     /gene="bclA2"
                     /locus_tag="ACGI57_003547"
     CDS             complement(30..1706)
                     /gene="bclA2"
                     /locus_tag="ACGI57_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HFG9718593.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2084..2623)
                     /gene="hpt"
                     /locus_tag="ACGI57_003548"
     CDS             complement(2084..2623)
                     /gene="hpt"
                     /locus_tag="ACGI57_003548"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFG9718594.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
BASE COUNT          694 a          676 c          428 g         1056 t
ORIGIN      
        1 attattttat aatcttttca tcatataaac tattgtattc tataaactga tacatatcca
       61 gctgcagaag tacctgtcgt gcctgaacca aatgtaaaac ttaaagtaac agctgctgct
      121 ctagcatcaa ctaaaaagct taaacttaca cttacatctg atgaagtaag tgtatttact
      181 gcttttgcaa taaagttatt ttgagctact ccattaaagt tagtagtaat tgacataaat
      241 cctgcaagtg aaagtgtggc acgtataccg aaagatacat tgtatatccc tccaactgca
      301 agatttatag tactacttgt tggaaaagtt atattagttc catttataaa tgatgaatta
      361 aatgtcacta atgtattatt cactaaactt gaagaggaca ctgtaaattg tgcattgttt
      421 gctgctaaca ctcctgttgc tccggtcgct ccggttggac ctgttgctcc atttgctcct
      481 gttgctccgg tcgcccctgt tgctcctgtc gctccatttg ctccagttgg gcctgttggt
      541 cctatcgctc ctgctactcc atttgctccc gttgctccta ctgctccggt tgggcctgtt
      601 gctccatttg cccctgttgc tcctgtcgct cctgttgctc ctgctactcc atttgctccg
      661 gttggacctg ttggacctat tggacctgct actccattcg ctccggttgc tcctactgct
      721 cccgttgggc ctgttgctcc atttgcccct gttgctcctg tcgctccggt tggacctact
      781 gctccatctg ctcccgttgg gcctgttgct cctactgctc ctgttggacc tattgcccca
      841 gctactcctg ttgctcctgt cgctcctgtt ggacctacca aaccatttgc tccagttgct
      901 ccggttgggc ctgttgggcc taccaaacca tttgctccag ttgctccggt tgggcctgtt
      961 gggcctgttg gacctaccaa accatctgga cctgttgctc ctactgctcc tgttggacct
     1021 acctctccat ctgctcctgt tgctccggtt gctcctattg ggcctgttgc cccagttgtt
     1081 cctgttgctc ctgtcgctcc tatacttccg gttggacctg ttgctcctgt atttcctgtt
     1141 gggcctgtag cacctgttac tccatttgct ccggttgctc ctgtatttcc tgttggacct
     1201 gttattccat ttgctccggt tgctccggtt gctccagtta gtccggttgg acctgttgct
     1261 ccagttgctc ctgtattccc tgttggacct gttattccat ttgctccagt cgctcctgta
     1321 tttcctgttg gtcctgtaga gcctgttatt ccatttgctc cagttgctcc tttgtttcct
     1381 gttggacctg ttattccatt tgctccggtt gctcctgtat tccctgtcgg acctgttatt
     1441 ccattcgctc cagtcgctcc tgtatttcct gttggtcctg tagagcctgt tgttccattt
     1501 gctccagtcg ctcccatatt tcctgttggt cccgttattc cattcgcccc ggttgctccc
     1561 gtatttcctg ttggacctgt tattccattt gctccggtcg ctccagttgc acctctaggc
     1621 cccgtcggtc ctgttggacc agtagcacct gttggccctg ttggacctac atcttgatat
     1681 aaacttggac ctgaaatatc actcatataa ttaatcctcc ttttttaaag ttagagtatt
     1741 actctataaa tattgagtat tactctatat aattatatga atcgacaatt tcttatgtga
     1801 taatgtatct atttgcttat atcaacatat atgctttgat tcttgaattt cattactttt
     1861 ttgtaaaatc ttttacaatt attagtactc ttttacaatt attaatacaa ttaaaaaact
     1921 cattttccat caattattta aaattttttt attatgttat aaaaaataga taattaaatt
     1981 gcataaaatt aatttaatac tactttaatt actttgtaaa taaaaaaacc tcaacacgat
     2041 taataaaaat catattaagg catccacaaa attatataat tgcttatcta tcttcatcag
     2101 taacattgaa tatataagga acatttctat aataatcacc aaaatcaaac ccatatccaa
     2161 ccacaaactt atcttctatt tcaaatccac aataatctgg cactaaatcg acttttcttc
     2221 tgcttggttt atccaaaaga acacaacatt ttaaactagc aggattttta gcttttaaat
     2281 gttttaacac aaaatccata gtaagtgcag aatcagtaat atcgtctaca accaaaacat
     2341 catatcctgc tatatcagta gtcacatctg aaactacttt aacccttcca gtactttctt
     2401 cattgtttcc ataactagat gtagtcataa aattaactct aagtggtaaa tttatatttc
     2461 taactaagtc tgcacaaaat ataaagctac cttttaatag agaaactacc ataagatttt
     2521 tatctttata atctttttct ataattgctc ctaattccct taatttatct ttaatttttt
     2581 cttctgaata caatacttcc catttttttg tttctatatc cataaaaaga cctccaattg
     2641 taataatcct tcttaatttt ataagagatt gctcactttt tcaatactct tattcaatat
     2701 tagtcaatat tattgagttt ttatgttcat tcaacaatta ttaacaatgt atcttatatt
     2761 ttaagtagtt catatatcca cactaaatta catactatat attttagaca aaaaaagaaa
     2821 aaacttgcac taacaagttt tttctattat acat
//
LOCUS       DAYAOX010000313         2851 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.317, whole genome shotgun sequence.
ACCESSION   DAYAOX010000313 DAYAOX010000000
VERSION     DAYAOX010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.317"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..545
                     /locus_tag="ACGI57_003549"
     CDS             <1..545
                     /locus_tag="ACGI57_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9718595.1"
                     /translation="INTIFDENKLREQLKTVLCLREGNIDTTNSTNLLINNLLDEDKF
                     MILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFDKGLLFPHSINEKS
                     DKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYEEVLKIGQDVKLTN
                     KISKCRTFLEFKKVLLKNLI"
     gene            614..973
                     /locus_tag="ACGI57_003550"
     CDS             614..973
                     /locus_tag="ACGI57_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFG9718596.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAVISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            1008..1364
                     /locus_tag="ACGI57_003551"
     CDS             1008..1364
                     /locus_tag="ACGI57_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFG9718597.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            1401..2693
                     /locus_tag="ACGI57_003552"
     CDS             1401..2693
                     /locus_tag="ACGI57_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFG9718598.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDIDLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         1140 a          249 c          558 g          904 t
ORIGIN      
        1 aaataaatac aatatttgat gaaaacaaac taagagaaca attaaaaaca gtattgtgcc
       61 taagagaagg gaatattgac actactaatt ctacaaattt actaataaat aatttgttag
      121 atgaagataa atttatgata ttaaatgaaa agactatact tgcctcatta gaaaaaatga
      181 tggaccatct tatggatttg ggatgtattg atgagaagtt tagaaaaaat atagtcaata
      241 gagaagaaaa atctcccaca gtatttgata aaggtttatt atttcctcat tctataaatg
      301 aaaaatcaga taagttttta atggcagttg gaattttaga agaaccaata gaatatgcaa
      361 atagaagtat aaaaataatt cttatgacaa tgtttccatg tgagaataag atagactcag
      421 acttattagt aaaaatttat gaagaagttt taaagatagg ccaagatgta aaattaacga
      481 ataagataag taaatgtaga acttttttag aatttaaaaa ggtgttactt aaaaacctta
      541 tataactatt gtacagcatt tatagttgtt atacagattt aaaaataatg tgatttttgg
      601 aaggtgtata aatatgaaat tattagtaat tttatttgtt gcttatattt taaatactat
      661 atttataatg aagcagaata aattgtattt taaaacttta aatagagcta aaaaaaaggg
      721 tgatatagtg tctacaggca aaaagaaaag ctatttttct aaaggaagta tagccgttat
      781 atcttcagat tcagatgggt ttataaagtt tggagaaatt ttaaaagggc gtacagtaat
      841 ggctaaattt aaagagattg aagaaataga aggattagat atatatagtg cagagcaaaa
      901 atttaaagat gaagagagta ttgtacaagc tataaaattt ataaaagaaa aaataaatgt
      961 ttcttttaat taactcaata taaatataaa ttatcaggag gaatgtcatg aaatatgaag
     1021 ttaagataag tgggataggc agtttagtta aagagttaat ggatgaaagc aattgtctta
     1081 ttatatatga tgaaaccatt aatgatgacg acttaaagga tatatctgta attcattcaa
     1141 tagcaacatt aaaaagtgat gttgaaatag gagatacact tactataggg aatagagatt
     1201 attgtatagt ttcagttggt gatattgctc agaagactct tagagaaatt gggcattgta
     1261 caatcaaatt tgatggaaaa tgtgaagtaa atttaccagg agaaatacat gtggaaatag
     1321 gaaatccaga tataactatt ggagatttaa taactatttc ttaatttgaa aataaaatta
     1381 ttataacagg aggaattggt atgttagatt taaacagaaa acttgcaaaa ttggaagagg
     1441 aaggtaaaaa aataaaagta gctcttatag gagctgggca tatgggaaat ggaatggtaa
     1501 gccaaatggc aaatatgaaa ggtattgaag cttctattgt tgtagatata gatttggagt
     1561 tggctcataa agcttttact gatgcaggaa tagatgaaga aattatagat aatgtaacaa
     1621 atgaaaagga tgcagaaata aaattacaag aaggaaaagt attgacatgt aatgactttc
     1681 ttgtggcttg taaaactaag tcaatagatg tagttataga tgcaactggg ggtattgcaa
     1741 ttggtgcaga gatagcatta aactctattt taaataaaaa acatatagtt atgttaaatg
     1801 ctgaaactga ttgtgttgtt ggaccaatac ttaaaaaatt agcagatgat gctggagtaa
     1861 tatttacagg ttctgcagga gatgagccag gtgcagtaat ggaattattt gattttgcag
     1921 atgcaatggg gtttgaagtt agagtgatag gtaaaggtaa aaataataaa ttagatttag
     1981 attgtaatcc tgatacagtt agagaagaag cagaaagaaa aggagcttca cctcatatga
     2041 tagcttcttt taaagaagga actaaaacaa tggtagaaat ggctttaatg tgtaatgcaa
     2101 ctggatttgt gcctgatgta agaggtggtc atggtattga agctacagtt aatgaagtac
     2161 ctaagaaata tgcattaaaa agcgaaggtg gagtattaga taattatggt gtagttgatt
     2221 ttgtaaatgg tatagcacct ggagttttcg tagtagttgc ccataaatta aaagcagtta
     2281 atgatgaact aaaatattta agtatgggtg atggacctaa ttacatatta tatagaccat
     2341 atcatttatg tagtttagag actcctttat cagcagctat ggcagtttta gaccataagg
     2401 ctacaatagt acctaaagct ggactagtag cagaagttat gactatagct aagaaagatt
     2461 tgaaaaaagg tgagtttatg gatggctatg gagcatatac atgctatggt actatagaaa
     2521 aatacgatgt agcaaaggct atgaatgctg tacctattgg tttgataagt aaaaagacta
     2581 aagttgtaaa agatattaaa aaaggtgaag ttataactta cgatatggta gagatagaaa
     2641 aagatactac tttatatcat ttaagacaat tgcaagaaaa aatatttggg taagatttga
     2701 tggtaatata aatagatatt agtaaaaagg gttgatggtt atttttaaat caaccctttt
     2761 aaatttttat atattcttgt taggatactt tctttaaaag tatattatag gttatttgta
     2821 gtcgtaaaaa ttattttgaa ttagttctgc a
//
LOCUS       DAYAOX010000314         2850 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.318, whole genome shotgun sequence.
ACCESSION   DAYAOX010000314 DAYAOX010000000
VERSION     DAYAOX010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.318"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            695..>2850
                     /locus_tag="ACGI57_003553"
     CDS             695..>2850
                     /locus_tag="ACGI57_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9718599.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAVTVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSGGSSSNQTSVNNEAAKITSVPTPAKDATRLTMPSVSSGYTIAIKTSSNE
                     SVIKKDGTIIPPNTATTVKLVFTVTHTSSGKTADTKELSVTVPAKSTDEELQAALDNE
                     VAKITSVPAPAKDATKLTMPSVSSGYTIAIKTSSNKSVIKEDGTIIPPNTEETVTLVF
                     TVTQESSGKTADTGEIDVVVPAKSTDGEIQAEVQAEA"
BASE COUNT         1226 a          357 c          493 g          774 t
ORIGIN      
        1 tgatttatgt aagatttatg accaatttgt gtatgatttg tgttctttaa tcatgacaat
       61 attatattgt aaaaaataaa tttaataaaa aatgacttgg aatttatatt ctaggtcttt
      121 tttatataaa aatttaataa gtttcaaatg ttggtaactt tgacagtaga aaacgtctat
      181 tgagataacg aggattctaa ttgaggtgga gagatattag cacttaaagt agtatatgca
      241 acagatataa tctttacaaa agtaatttga attaaaatgt aaatataaag aataattaaa
      301 aaaatatcgc aattatttgt ttttcatatg gataaaattg gttatttaat atataattac
      361 aagtataaat aataaaattt atttgtaaaa agagtagcac ttttaattct aaaggctact
      421 ttttttatac aaaaccctat tttttataac aattcattaa cataaaaatc aaacttacct
      481 taaaatataa atttacaaat ttttcaatca ataagaaaat ttctttattc cttacataat
      541 gactgcaatg taacaaaata attacaaatt aattatgaaa tcattatagt tagaaacctc
      601 tttaaaatta gatattaagt atcctttaga attagaacgg gaacaaagtt aaaaaattaa
      661 gtaatatcaa atggacaagg gggaagaaaa taaaatgaaa ataccaaaga agattgctgc
      721 aatgcttaca gttacaatga tagctggaag ttcaacagca ggcatagcta gtgcacaaac
      781 tgtggcaaca aatttaacag ggcaagaaag atatgaaact gctgttaaaa taagtcaaga
      841 tggatggaaa aatgctgatg aggtagtaat cgtaaacgac tcatctatag cagatgctct
      901 atctgcaaca ccatttgcaa aagcaaaaaa tgctccaata cttttaacaa gtaaagataa
      961 attaaatgat aagacgaaag cagaaataca aagattaaaa gctaaaaaag tatacttaat
     1021 aggtggaact tctgtattaa gtacaaatat agaaaaagaa ataaaagatt taaaaatatc
     1081 ttttgagaga atctctggag ctgagagata tcaaacatcc ttagaattag caaagaaatt
     1141 agatgctata agtgatgtta agaaaatagc tgtagttaat ggtgaaaaag gacttgcaga
     1201 tgctgtaagt gtaggagcac cagctgcaca aaacaatatg ccagtaatac ttgctgattc
     1261 taaaaatgga acagcagttg cagacaaatt tataaaggat gctggaataa ctcaatctta
     1321 tgtagtaggt ggagagagtt caatttctga agctgttaaa aataaactac caaatagtac
     1381 aagactaggt ggaacagata gaaatgatac aaatgcaaaa gtaataaaag aattttataa
     1441 aaagacagat ttaaaaaatg cttatgtaac taaagatggt atgaataaac aagaccaact
     1501 tatagatgca ctagcagtag gtgtattagg tgctaaaaat caatcaccag tagttttagt
     1561 tggaaagaac ttatcagctt cacaaaaatc tctagttaac tctaagagtt ttgataagat
     1621 aactaaagtt ggtgggaatg gtaatgagac agcatttaat gaaatgaagt cattacaaga
     1681 agtaaaaaca gttgaagcta aaacaatttc tgaattaaaa tcagctatag ataaagctac
     1741 tgctaatgat gttataaact ttaagccaac tagtgaggtt aaagaagcat ttacaataca
     1801 aactgataaa gcagtaacag taaacttaaa tggtacatat acaaagactg tgactatcaa
     1861 catgcctaat ggtgatgtaa ataactatgc aaaagtggat gatgtagtta tagatgatgt
     1921 aaaagatgga acttttgtta attatggaaa aataactaac ttaaaggtta atgataaaaa
     1981 tggagccaag atagaaaata attctaaagg tgagattggt agtttaacag tagcatctgg
     2041 agcaagtcaa gtaaaagtga ctaatggtgg taagataact acagttacaa ataattctaa
     2101 agggactact atagataata aaggtacaat aagtagtgtt aagggagata attctccaac
     2161 aataagtgga aatagtccaa gttctaactc ttcaggagga agttcatcaa gtggaggttc
     2221 ttcacatgga ggaggctcat cttcaggagg aagttcttct aatcaaacat cagtaaataa
     2281 tgaagcagca aaaataacaa gcgtgcctac accagcaaaa gatgcaacta gattaactat
     2341 gccatcagta tcaagtggat atactatagc aataaaaaca tcaagtaatg agagtgttat
     2401 aaaaaaagat ggaacaataa tacctccaaa tacagcaaca acagtaaaat tagtatttac
     2461 ggtaacacat acatcaagtg gtaaaacagc agatacaaaa gaattatctg taacagtacc
     2521 agctaaatca acagatgaag aattacaagc agcattagac aatgaagtgg caaaaataac
     2581 aagcgttcct gcaccagcaa aagatgcaac taaattaact atgccatcag tatcaagtgg
     2641 atatactata gcaataaaaa catcaagcaa taagagtgtt ataaaggaag atggaacaat
     2701 aatacctcca aatacagagg aaacagtaac attggtattt acagtaacac aagaatcaag
     2761 tggtaaaaca gcagatacag gagaaataga tgtagtagta ccagccaaat caacagatgg
     2821 ggaaatacaa gcagaagtac aagctgaggc
//
LOCUS       DAYAOX010000315         2838 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.319, whole genome shotgun sequence.
ACCESSION   DAYAOX010000315 DAYAOX010000000
VERSION     DAYAOX010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.319"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(153..1097)
                     /locus_tag="ACGI57_003554"
     CDS             complement(153..1097)
                     /locus_tag="ACGI57_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9718600.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            1415..>2838
                     /locus_tag="ACGI57_003555"
     CDS             1415..>2838
                     /locus_tag="ACGI57_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFG9718601.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYT"
BASE COUNT         1130 a          420 c          347 g          941 t
ORIGIN      
        1 gtctaaactg tatctactta tattttattt gtactgtcca ctttattcta taaaatttat
       61 aaatagttat tttttaatac caaatataat ttggtataat ttttgttgga atctacaatg
      121 tataccaata aatccttttt aagtatataa ttctaaatac tttcagtttt ttcaatctgc
      181 tttaacttgt tggacttata aaatattctt atttcatcat aagaaagatt aagtgctact
      241 actgcagata ttaacatcaa catcactcca aataacttcc ctaaggaaaa actttctcct
      301 aatatagtgc aaccaattat tactgaacca actaattcta ctgatgccac aacacctgca
      361 actgacaact ctacaccttt agatatacct gctgcataaa aaatatatgc aagtgcagct
      421 ggaacaatgc ctaacattag catacaagat aaaacatcta aatccttagc ataatttaag
      481 atttcccaag gtcttgaaga aggaatcata aatatagctc caaataagaa actgtatact
      541 aatattgttg aacttgaaaa ttcctttaaa gcatttttac taatagttgg cataagtgca
      601 tatgctattg ctgataatac tccaagtaaa agcccaaaag cgtttaaacc ttgaaaatcc
      661 aaacgtccac cagtcactgc cataattgca ccaataaaac aaagaataag tgaaaataat
      721 ttgcttctag taatattttc tttataacat aattttgaaa atattgctaa aaacagtggt
      781 gatgtatata gtaaaactgc tgcaattgat acacccgcaa tgtcaattgc tttaaaataa
      841 cataaattaa acattgcctg acatataatt cctattatta ctgaatatat aattcctttt
      901 ttgtttatct ttaagatttg tggtgtcctt atagatgaat atacgcctaa aactataaat
      961 ccaaaaaaca atcttgtaaa tgctacttgt tctgatgtca gtccatttcc cattaaaaac
     1021 tttccaaata atccaagtgt agcccataat aaccctgcta ttgctataaa tacataccct
     1081 ttactttttt ctcccatttt aaattccctt ctatttttat ttagtcaatt tacactcttc
     1141 tttttaagca aaaactatgc catcttaaat caatatatat cgactctaaa accctagtct
     1201 ttccaataat actaataaat tttcaatttt tttatattta tttttcgctt actttaagtt
     1261 gatttagact tattttaatt gtaaaatgag ttatatataa cttatttaaa gttaaaatag
     1321 actgaaactt atatataaat atcaaatttt aagtatatta atcaattaaa cctattttaa
     1381 tatatcatta aaaatttagg aggaaaatat aaaaatgaat ttagatttgg aattttatca
     1441 aaaaatatta gaagcttccc atgatgaaat atgtgtttct gatgataaag gaataataat
     1501 atattgtaat aaagcatttg aagaaaatta tggtctaaaa aaagaagata ttttgggaaa
     1561 aaatgtttct tttttagaag actctggata ttcaacaaaa agccccatac caattgtatt
     1621 aaaaacaaag actaaatttt cactggaaca agatactcaa acagggaaaa agcttatcat
     1681 aactgctacc cctatatttg atgaaaatgg acacttagaa tttactgttg aaaattgtag
     1741 agatataact gaacttaata atataaaaaa taaacttgaa gacacaaaaa agcaagtaaa
     1801 aaaatataaa tctgaagtag aaacactata cagaactgca cttagaattg aagatactgt
     1861 aattatggac ggaatagtta tgagaccaat cataaacaca gtaaatcatg tttctaaaac
     1921 ggatgtaagt gtacttttat tgggtgaatc tggaacagga aaaagctcac ttgctagata
     1981 tatacaccac aacagcaata gatctaatgg cccatttata acaataaatt gtgcaactat
     2041 atctccacaa cttcttgaat ctgaactttt tggatatact tcaggtgctt ttacaggtgc
     2101 aagtactaaa ggtaaagttg ggctcgtaga attagctaat ggaggaactc tttttttaga
     2161 tgaaatagga gatataccac aaaatctaca ggcaaaattt ttgcaattaa tacaggatag
     2221 aacttttact ccagttggga gcttaaaaaa taaaaaggtc gatataagaa ttatatctgc
     2281 tacaaatgtt gatttagttt caaaagtaaa agaaaaaaag tttagagaag atttatacta
     2341 tagattaaat gttatagaaa taaaattgcc tcctcttaga gaaagaaggg ataatctagt
     2401 ggaaattata aaatattatt ttaataggta ctcaagtgac ttcaacctaa ataaaactat
     2461 ttctaaggaa gctatggatg ctattgcaaa ttatagattc ccaggcaata taagagaatt
     2521 acaaaatata attcaaaaaa tcttgcttac ttgtacagat aatcatataa caatagatga
     2581 cttaccaaac attcttacaa aaaatataca tattaccaat aatggaaata aaacacatat
     2641 atcccaaatt aataaggtta ttatatctga ctcaaaatct actaattata aaaataaaaa
     2701 ttttgacact ttaattaaag agtatgaaaa aaatataatt ttggatgctt atgaaaaatt
     2761 tggtagttct tataaagtag ccaaacacct tgaaatctca cagtctaaag ccaataggtt
     2821 aattagaaag tatactaa
//
LOCUS       DAYAOX010000316         2793 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.320, whole genome shotgun sequence.
ACCESSION   DAYAOX010000316 DAYAOX010000000
VERSION     DAYAOX010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.320"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..597
                     /locus_tag="ACGI57_003556"
     CDS             <1..597
                     /locus_tag="ACGI57_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718602.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            694..1731
                     /locus_tag="ACGI57_003557"
     CDS             694..1731
                     /locus_tag="ACGI57_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9718603.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            2540..>2793
                     /locus_tag="ACGI57_003558"
     CDS             2540..>2793
                     /locus_tag="ACGI57_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718604.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
BASE COUNT         1064 a          361 c          426 g          942 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaaatt actttagata gtactactat aacaaattta
       61 gctgagtata aaagaactca aatagtaagt agagtttttc aagacccaag ccttggaact
      121 tgtccttcta tgacggttag agaaaattta tctctagcac ttaataaagg gaaattattg
      181 aacttaaaaa aatgtcttcg tcataaaacg aatgacttgg aacacttact tgaaggaatt
      241 tctttggatt taaaaaaata tttagatatt aaagtacaat atctttcagg gggtcaaaga
      301 caatcactat cactaattat gtctagctta gcaagtccta aggtcttatt actagacgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa tagagttaac agataaaatc
      421 gtaagagaga aaaatataac tacacttatg gttactcaca acttgaaaca cgctcttcaa
      481 tatggagata ggttgataat gcttcataag ggtgaagttg tattagatgt aaaagataaa
      541 gaaaaaaaag atttaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtataagaa
      601 attaaaatct tagttttaat cagaaatatt tataatctat catatgaata tatgaacaaa
      661 tataattatt aaattttaag ggaggaaaat aaaatgataa aaagtaaaaa aatattaagt
      721 ttaattatag caggagtact tggtgtatca atgcttactg gatgttctca aaatgatggc
      781 tccaatgctt caaatgaaaa taaagaaaca gatagcaaaa aacaaaaaaa tataggtatt
      841 actcaattag tggaacatcc atctctggat aaagcaaaga aaggattcat caaagcactt
      901 gaagataaag gctataaaga tggagataat ataaaaatag atttccaaaa tgcacaaaat
      961 gatatgccta ctacacaaag tattgctagt aagtttgtat ccgacaaaaa agatttgata
     1021 tatgctatat ctactccatc tgcacaagca gcttataatg ctactaaaga tattccaata
     1081 ataatgactg ctgttacaga ccctgtagaa gctggacttg ttaaatctct tgaaaaacca
     1141 ggtggaaatg tttctggtac atctgattat ctttcaattg ataaaacatt agaattggtt
     1201 aaaacattga ctccaaaagc aaagaaaata ggggttatat acaatactag tgaagttaat
     1261 tcaaaaatcc aagttgattc tctacatgat tatgctaaga aaaataatta tgaagtagtt
     1321 gaaaaaggaa tcagctcttc aagtgaagtt aaccaagcta tttctagttt agttggtaaa
     1381 atagatgttt tatatgtccc tactgacaat ttaatagttt cttctatgcc aatagtttct
     1441 aaagttgcta atgaaaacaa gattcctata atagcctctg aagaaggttc tgtatcttct
     1501 ggtgctttag cttgttgtgg aatagattat gaaaaactag gttataaggc tggagaactt
     1561 gctattgaag tattagaagg aaaatctgtt ggcgatatac cagttactac attagatgaa
     1621 actgaaataa taattaacga agatacacta aaagcacttg acatgcaaaa gttatcagca
     1681 gataatataa agtatataaa gtcagatgaa aatgcaaaat ctacaaaata aaacttatta
     1741 atttcaaaaa ataggtaata tgaagtaggt aatatgaaaa ctttatataa taagtactct
     1801 tatccaccct aaccctttga aaattgtgct tattgtagca atttgaatat tgcatataaa
     1861 gttattcatt ttccataaaa gtcgtctata ttgacgactg attatgaaag ttatataact
     1921 ttctaatcac tacttgagct gtccaaaaga attttaatat tctccagaca gctttttttt
     1981 atgatatttg attgatatac atgtctagtt attccatatt tagtaaatat tttgataaaa
     2041 tttccacatt cacttataac ttaggataac gtttttttca aacaatacat taatttaaaa
     2101 gtattctgag tatacattat ttagtatttt aattaaattt agcagttgac ttttattatt
     2161 gtataattta taatttataa caagtcgaga atacatataa ttttctgact aattaaaata
     2221 ttaaaaggga gagataatta tgaatacttt attaaacaat agaaaatatt attattatta
     2281 tagccactat ttttagggtt tgtacctacg ttttaacata gatacagacc ccttaagact
     2341 actaaaattt tgggattgta tctatgttgg ctatattgtt tataaagttt tcatctaact
     2401 atataagagc cacatagatt aaactatgtg gcttattcgt gtttttacca catagttttg
     2461 tacactatgt ggttttttta tacacaaaat tagcaaataa ttaaaattat aaaaatattt
     2521 tcaaaggagt gttttattta tgtcaggtat tatatctgta atgacgcaaa gtttaatttt
     2581 atcgattatg gcactggggg tttatataac ttacaaaatc ttagattttc cagatatgtc
     2641 tgctgatggt agctacacaa tgggtgcttc tattgtagca ttttctctta ctaatggtat
     2701 ttctcctgta gtagcaactt taatggctat cttatgtggt tgtactgctg gtcttgttac
     2761 agggattctt cacattaagt ttaagatatc taa
//
LOCUS       DAYAOX010000317         2791 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.321, whole genome shotgun sequence.
ACCESSION   DAYAOX010000317 DAYAOX010000000
VERSION     DAYAOX010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.321"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            236..>2791
                     /locus_tag="ACGI57_003559"
     CDS             236..>2791
                     /locus_tag="ACGI57_003559"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFG9718605.1"
                     /translation="MTFEEIMEFNKPYNQKNFDELVNAIKDGYVVPYIGAGMSMLFKD
                     IYPSWSTFLNNTSEEYLEDEDKILFEKIEDYEKKAEFLYEELAKVRFSKHLKKVFGAG
                     YLDAKDKDDFIDKCVYLIPIIFGSGLIITTNYDKVLEKNYGLHNKTVLVSHPGHFEAL
                     NNALRNNELVLYKIHGDITEPTTSIVLTKEQYEKAYSNDELRKMLTQVYTSKELLFLG
                     CSLEKDRPLELLQQVSEAGTGHYAIIPVSSEKKKHRRIELENEYYIQTIMYPEGKHEC
                     VRIILERILEIVLPDEYRRLKDRSLKESRLTNLSCRLTEKWFQNQNNIQIKNLGDRYL
                     PELNVELSLENTFNALGKNEEFKQRFDEKTDKVLSSIKKQKINEVEVDVLVIEEIIQQ
                     FYDKANERLDIKLLKKHFEKISAALDDRINNFKNIIINEDCEKSKKREDEIYKLKQTQ
                     KDIYVYIKYLDSKEIKVVNSPYILLHSAGGMGKSHIIAHTIEKRNMDGHKSILFLGQH
                     FKLDKTPIKHMLEILEIECEFEELLEILNEIGEKEQQRVILFIDALNEGQGKELWKYY
                     LNGIVEQIKKYPWIGLILSIRSEYIPSLIDNNNKIKENLVFIEHQGFSSIEHKAMKKY
                     FDYYGIPYSNIPMPNQEFRNPLFLRIMCETFKGEEINLDTISFTDIYKNYLNILNLRI
                     SESCNYSRHANVVTEVLNEMIKYKYGDGLGNNLIPANKSVEIIINIASKYKINNVIID
                     ELIANGIVNKTVNLNDEEYMHVTYEKLEDYLYANLLVKELKEIGVVDFSHKYKYLVEY
                     EDILEVLAIVLSEETGSDIPNEIFEVFKNKETNYNVISAFINALKWRKKSTITE"
BASE COUNT         1192 a          233 c          484 g          882 t
ORIGIN      
        1 aaaattgtta atattagtaa tacattaaaa gttataacat tccattttta tccataattt
       61 aaaagtataa attttaatta tcttaaaaga aagttttata ttattaacaa atgtaagtaa
      121 gtgaattcat tctataacat agaattgtag taaaaaaatg gaatggaatg atataataaa
      181 attataaaat aggaattatg gttgaaatta ttaagagata gtttaggagg gaataatgac
      241 atttgaagaa attatggaat ttaataagcc ttacaatcaa aaaaattttg atgaactggt
      301 aaatgcaatt aaagatggtt atgtagtccc atatattgga gcaggcatgt ctatgttatt
      361 taaagacata tatccttcat ggagtacatt tttaaataac acaagtgaag aatatttaga
      421 agatgaggat aaaattttat ttgaaaaaat agaggattat gagaaaaaag cagaattttt
      481 atatgaagaa ttggcgaaag ttagattttc taaacattta aaaaaagttt ttggggcagg
      541 ttatctggat gcaaaagata aagacgattt tattgataaa tgtgtctatt taattcctat
      601 aatttttggc tcaggattaa ttataactac taattatgat aaagtactag aaaaaaatta
      661 tggattacac aataaaacag ttttagtgtc acatccaggt cactttgaag cattaaataa
      721 tgcacttaga aataatgaat tagtattgta taaaattcat ggggatatta cagagcctac
      781 aacatctatt gtgttaacaa aagaacaata tgaaaaagca tatagtaatg atgaattgag
      841 aaagatgctt acacaagtat atacaagtaa agagttgtta tttcttggat gtagtttgga
      901 aaaggataga ccgttggaat tattacagca agtatcagag gcaggaacgg gtcattatgc
      961 tattattcca gttagtagtg agaaaaagaa acatagacga atagagttag aaaatgagta
     1021 ttatatacaa accattatgt atcctgaagg taagcatgag tgtgtaagaa taatacttga
     1081 acgaattttg gaaatagttt tgccagatga atatagaaga ttaaaggata ggtctttaaa
     1141 ggaaagtagg ttaactaatt tatcttgtag attaacagag aaatggtttc aaaatcagaa
     1201 taatatacag ataaaaaacc ttggagatag atatttacca gaattgaatg tagaattgag
     1261 tttagaaaat acgtttaatg cattaggaaa aaatgaagaa tttaaacaac gttttgatga
     1321 aaaaacagat aaagttctat caagtataaa gaaacagaaa ataaatgaag tggaagtaga
     1381 tgtactagtt atagaagaaa taatacaaca attttatgac aaagctaatg aaagattaga
     1441 tatcaagttg ttaaaaaagc actttgaaaa aataagtgca gcacttgatg atagaataaa
     1501 taattttaaa aatataatta taaatgaaga ctgtgaaaaa agtaaaaaaa gagaagatga
     1561 aatatacaaa ttgaagcaga ctcaaaaaga tatctatgta tatataaaat atttggattc
     1621 taaggaaata aaagtagtta atagccctta tattttatta catagtgcag gtggtatggg
     1681 gaaatctcat ataattgcac atactattga gaaaagaaat atggatgggc ataagtctat
     1741 attattttta gggcaacatt ttaagttgga taaaacacca attaaacata tgttagaaat
     1801 attagaaatt gagtgtgagt ttgaagaatt attagaaata cttaatgaaa taggagaaaa
     1861 agagcaacag cgtgtgatat tatttataga tgcattgaat gaaggtcaag gaaaagaact
     1921 ttggaaatat tatttaaatg gtattgtaga acagataaag aaatatccat ggattggact
     1981 aatattatca ataagaagtg aatatatacc atctttaata gataataata acaagataaa
     2041 agagaatttg gtttttatag agcatcaagg attttctagt atagaacata aagctatgaa
     2101 aaagtatttt gattattatg gaatacctta ttctaatatt ccaatgccaa atcaagaatt
     2161 tagaaaccct ctttttttga gaattatgtg tgaaactttt aaaggagaag agattaactt
     2221 agatactatt agttttacag atatttataa aaattatttg aatatactta atcttagaat
     2281 atctgaatca tgcaattata gtagacatgc caatgtagtt acagaagtac ttaatgaaat
     2341 gattaaatat aaatatggag atggtcttgg gaataattta atacccgcaa acaagagtgt
     2401 agagattata ataaatattg caagtaagta taagattaac aatgtgataa tagatgaact
     2461 catagcaaat ggaatagtta ataaaacagt caatttaaat gatgaagaat atatgcatgt
     2521 gacatatgag aagttagaag attatttata tgcaaatttg ttggttaaag agttaaaaga
     2581 aattggggta gtagattttt cacataagta taaatattta gtagaatatg aggatatatt
     2641 agaagtattg gcaattgttt tatctgaaga gacaggctca gatataccta atgaaatttt
     2701 tgaagttttt aaaaataaag aaacaaatta taatgttatt agtgcattta tcaatgcact
     2761 aaaatggcgt aagaaatcta caattactga a
//
LOCUS       DAYAOX010000318         2770 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.322, whole genome shotgun sequence.
ACCESSION   DAYAOX010000318 DAYAOX010000000
VERSION     DAYAOX010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.322"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            126..1640
                     /gene="glpK"
                     /locus_tag="ACGI57_003560"
     CDS             126..1640
                     /gene="glpK"
                     /locus_tag="ACGI57_003560"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFG9718606.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(1754..2383)
                     /locus_tag="ACGI57_003561"
     CDS             complement(1754..2383)
                     /locus_tag="ACGI57_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HFG9718607.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
BASE COUNT         1060 a          306 c          496 g          908 t
ORIGIN      
        1 agtgtcttct tactaaatat ctattatatt aaatttgtac ttaagtatga ttatttattg
       61 aaatatgtga tattaaattt ttctttttaa taaattaaat attatatgat aaggagaata
      121 tggtgatgga aaagaagtat gtaatggcat tagaccaagg aacaaccagt tcaagagcta
      181 tactatttaa taaaaaaggc gaaatagtga aaattgcaca aaaagagttc aatcaaattt
      241 atccaaaagc tggatgggta gagcatgacc ctatggaaat atgggggtca caaagtgggg
      301 taatgagaga agttatagaa acagcaggta taagaccaga agaaattgct tcaataggga
      361 taactaatca aagagagact acggttgttt ggagtaggta tactggtaaa cctatttata
      421 acgcaatagt atggcagtgt aggagaacgt cagaaatatg tgatgaactt aaaaataaag
      481 gattggaaga atcaataaaa gaaaaaacag gtcttttaat agatgcatac ttttcagcaa
      541 caaaagtaaa atggatatta gataatgtag aaggagctag ggaaaaagca gaacaaggag
      601 agttattatt tggaactata gatacatggt taatatggaa tttaactcgt ggaaaagtac
      661 atgtaacaga ttactcaaat gcatctagaa caatgatgta caatataaat gatttagaat
      721 gggatgagga tatattacga gaattggaca taccaataag catgttgcca ttagtaaaac
      781 cttctagcta tgtttatggt catacagatg agcgcatgtt atcaggagct aaaataccta
      841 ttgcgggatg tgctggagac caacaagcag cattatttgg tcaaaattgt gttgaagaag
      901 gtactgcaaa aaatacttat ggaacgggtt gtttcttgct tatgaataca ggcagtaaca
      961 tagttaaatc taagcatggg ttacttacaa caattgcttg gggagttgat ggaaaagtaa
     1021 catatgcatt agaaggaagt atatttattg gaggagcatc tgttcaatgg cttagagatg
     1081 aattaaagat aattgaatct gcaaaagata gtgaaatgta tgcaaatagg gtagaggata
     1141 ctaatggtgt atatgtagtg ccagcattta caggtcttgg agctccatat tgggatatgt
     1201 atgctagggg ttcaatttta ggacttacaa gaggagcaaa aaaagaacat atagtaagag
     1261 ccacattgga atcaattgct tatcagacaa aggatgtttt agaagcaatg caaaacgatt
     1321 caaaattaaa actaaaatca ttaaaagttg atggtggagc aagtaataat aattttttaa
     1381 tgcaatttca atcagatata ttaaatgtag atatagatag acccaaaata gtagaaacta
     1441 cagctttagg agctgcttat ttagcagggt tgtctgttgg attctatatt ggaagaaatg
     1501 aaattacttc aaagtggtca gtagaaaaag agtttaatcc aaatatgagt gaagagaaga
     1561 gatgtaaatt atataaagga tggaaaaaag ctgtatcgag agctttaagc tgggaaaaag
     1621 aagatgaatt ggatatttaa aaattaatca aagttaaatt tatttgaatt taaaaagagc
     1681 tttatgtaaa atatgaagct ctttttttgt tgttaatctt gcatttttat ataatcacac
     1741 taaattatat aatttatttt tcgttttcat ataaaaacat tggtatagaa ttgtaaatgt
     1801 gttggtcacc aactactaag agaatgtctc cttctaaaaa ttcaatatat ggtccaggag
     1861 atattataag ttcttcacct cttttaattg caactattgt agcacctgta ttttgccaaa
     1921 atttagtttg tgcagctgtt tgaccaatat atttgcaatt agaatttatg gtgaactgaa
     1981 cgggcactaa aggattgatt atttcaagtc tgttagaata gtctaaaatt ttatttatag
     2041 tatctaatat ttcattttct aaattagatt tagtatctat taaattataa agtgttgatt
     2101 tatatgatga tatatttgta atatctctat atttttttat aaaagaagaa gctttgtctg
     2161 gagatagtac aataacacct ttacccttta tgcttttaac aacgccaatg tcctctaaaa
     2221 ttttgacagc tcttcttatc gtttctggag atacattgta tttaccagcc aaaacagagc
     2281 gtccaaatag tactgaatcc tcttgtattt cacccgtgta tattttattt gcaatatcca
     2341 acgctatttt ttggtatatt ggcatcgtat agtttttatc cattgttttt cccccaatac
     2401 aaaatttatg ttaaaaaaat atataatctt aacaatacta ttgtataatt tataggttta
     2461 tatgtctaca atatgtacaa atattgtgca tgttttaaaa taatatttta acatgcattt
     2521 agtatacatg attttattat acttaaaact atataaagaa ttaattataa aaatataaag
     2581 atattttact tacaaagaat gataattata tagatgaaag aaacaaatga atgaagaaaa
     2641 atgattctgt cttgcatata aagtgattaa aaatgaaaaa tactttaaaa atgaaggtta
     2701 aagcccttat tttaagtgaa aataattaat tattaactaa aaaatggtaa aaagattttt
     2761 tgaaaaagaa
//
LOCUS       DAYAOX010000319         2696 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.323, whole genome shotgun sequence.
ACCESSION   DAYAOX010000319 DAYAOX010000000
VERSION     DAYAOX010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.323"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..1275
                     /locus_tag="ACGI57_003562"
     CDS             <1..1275
                     /locus_tag="ACGI57_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HFG9718608.1"
                     /translation="ISVIRWIFSIHEITLNTENFKRARKESVNLIKGKIIRTIIYSLI
                     LSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCIAFEEYFRLILSIFIFI
                     LDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILLISYIESVAFSSHINGM
                     FNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEIDVQETKDGELIVLHDS
                     NFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVGEKIPTLKEMIKYSKGK
                     VKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKTILQKVKKLDENLLTCY
                     FMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFVWTVNSDSLIEKMLDLN
                     VDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            1597..2181
                     /locus_tag="ACGI57_003563"
     CDS             1597..2181
                     /locus_tag="ACGI57_003563"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFG9718609.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKGLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         1064 a          244 c          430 g          958 t
ORIGIN      
        1 atttcggtta ttagatggat ttttagcatt catgagataa ctttaaatac agaaaatttt
       61 aaaagagcaa gaaaagaaag tgttaatctt ataaagggaa agattataag aacaattatt
      121 tattcattaa tattatcttt agtagtagct attattggat atgcgattta tcatagtgga
      181 attatattta taggcatatg gactaaatat tgtactgata tggaattttt gaagaatgta
      241 tttataaata gatgtattgc atttgaagaa tattttagat tgattttatc aatattcatt
      301 tttatattgg acattggatt tatctcagca acttattatc aatataaagg agtaaatatt
      361 tctaaaaata atattaaaaa agagaaaaaa tctatatcaa aaattgtttt taatttagta
      421 ttaatattat tgatttctta tatagagtca gttgcatttt cttcacatat aaatggtatg
      481 tttaatacgt cattttcatt ttatacaaca gcaattgctc atagagcagg agctgaaatg
      541 gctccagaga atactttggc agcaatagaa gaagcttcac atgctaaagc tgattatgca
      601 gaaatagatg ttcaagaaac taaagatgga gaacttattg tacttcatga ttcaaacttt
      661 aagagaacta caggagtaga taaaaatgtt tgggatgtaa attataatga agtaaagaca
      721 tatgatgcgg gaagctttta ttattatgga aaaggttatt atgatgaaaa gtttgttggg
      781 gaaaagatac caacactcaa agaaatgata aaatattcta aaggtaaggt gaaattatta
      841 atagaaataa aacttaatgg acatgaaaag ggagacgtag taaaaaaagt tgttcagtta
      901 attaaagaaa ataattttga aaatcaatgt atagttgcat ctatggataa aactatatta
      961 caaaaagtaa aaaaactgga tgagaattta ttgacatgct attttatggc aattgcatat
     1021 ggtgattttt caaagttaaa ttatgttgat atatatggaa ttgaatcaac ttttgttaat
     1081 aaaacagtag tagaaaagat tcataaaatg aacaaaaaaa tcttcgtttg gactgttaat
     1141 agtgacagtt taattgagaa aatgcttgac ctaaatgtag atagtattat tacagataat
     1201 ccatacctta tagagagtgc aatttattgg aagaaaaatg gatttattag tcaagtgtca
     1261 gattatttat tttaaatatt ctataattgt attatccagt aaaagagata ttattaaaat
     1321 caataatatc tctttttaac ataaataagg ttgttaaatt aaggttattc ttataaaaat
     1381 tgtgcttatc agctataaac aaggaataaa agtgaaaaaa ttgctctgag attattacag
     1441 ttaaaagagt tttaagatga cagagagata gattgtcaac tgtatattta ataaatcttg
     1501 ttatttatta aattttatat aagtaaaata tataaattaa taaatcttat gctagaatat
     1561 atatgaaaaa caatataaat ttggaggatt aaaaacatga cagagcgaga aaaaatgttg
     1621 gcaggagaac tttacgattg tggtgatatt gagttgttaa ctcaatggca taaagcaaaa
     1681 ggcctagtaa gagattataa ccaaacagat tcctcggatt tggaaaaaaa agaaaaaata
     1741 ttaaatgagt tacttggtgg taagggtaaa aacttatgga ttactgcacc gtattatgta
     1801 gattatggaa ataatattta ttttggaaat aattgtgaag tcaatatgaa ttgtacgttt
     1861 ttagatgata ataaaattat cattggggat aatgcactaa tcgcaccaaa cgtacaaata
     1921 tatactgcat ttcatccaac taatgctggt gatagatttg gtaatgccaa agaagatggt
     1981 tcatttgaat tttgtaaaac tcaaacagct ccagttacta ttggagataa tgtttggatt
     2041 ggcgggggtg ctattatcat gccaggtgtt actattggag ataatgtagt tataggtgca
     2101 ggaagtattg ttacaaaaga tattccaagt aatatgattg catacggaaa tccatgccgt
     2161 attattcgag aaaataaata aagttaaatt ttcttgcaaa atctcaaaaa acacctttct
     2221 tactttatac atagggtatt ttctaatact ttcacatcca tattcatttg ttaaattttt
     2281 gtggaatgta gtttttatca cttctatctt acatttccac cattttttta taaaagtcat
     2341 tcctttatag atttatttat aatagtatat attctatttg atgtttgaat aagcacttat
     2401 ttttaagtaa aatactaact tttaggttag tattttgcta tagtatgatg attaagtaag
     2461 aataaaatac tataatacat aaagcttaat aaatataatt tttattttac ttagatattt
     2521 tcttttgatt agcaatcaaa agtagttata atttttttaa ttaataagag taacatttat
     2581 tttatgaaag aggattatat taatatatat ttaaatagga aaaattgtaa aaagcataaa
     2641 aataatataa ttaattaatg agataaaagt ggataaaaat attgggagtt taattt
//
LOCUS       DAYAOX010000320         2689 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.324, whole genome shotgun sequence.
ACCESSION   DAYAOX010000320 DAYAOX010000000
VERSION     DAYAOX010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.324"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            83..2596
                     /locus_tag="ACGI57_003564"
     CDS             83..2596
                     /locus_tag="ACGI57_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9718610.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
BASE COUNT         1062 a          282 c          447 g          898 t
ORIGIN      
        1 atttatatat ccttttatat actaatctat ttttacatgt ctacaagttt atgcagtaat
       61 ttatactgta aatcttaact atatggagga ttgtgtagtg aatttaaaaa aattactttt
      121 attaattatt gtaattacat ctatattcac ttcttgcata ttttcttttg gtatcaacgc
      181 tataggtaat gataatcgta ccattcgtgt tgcttatcct attcaaaaag gtttaactga
      241 aatagatgag caaggtaact attttgggta tacatatgaa tatttactag aaattgcaca
      301 gtatactgga tggaattatg aatttgttca agttcctgga gagataaatg aatctattac
      361 aaaattaatg aagatgttag aaaatggtga aattgatata atgggaggta tgttatacaa
      421 tgagccaatg caaaaaatat ataattatgc aggaaatagt tatggagtag caaatacagt
      481 actacaagca ttatatgaaa atacagatat tgataacata atgagttcac aagatgtgaa
      541 aaaaatccgt attgctgtaa tagaaaattc tgatactaga cttaaagagt tagatgagta
      601 ttgtaaaatg aatcttatgt cacctgaatt ggtatattgt aaaacaaatg atgagcaact
      661 agaagcacta aagagtggta aagcagatgt acttcttaat gttagtatga acccactaga
      721 aggtgtacgt actgtagcaa aattttcatc aaaaccattt tatttcatta ctcctaaagg
      781 tagtacaaat ttaactcaaa agcttaactc agcaattaat aatattgaac agaaagaccc
      841 atatttttca actactttat atgaaaaata ctttacacca gagaacaatc aaatgttttt
      901 atctgatgaa gaaactgatt acattaaaga gacagaaaaa ttaaaagtag gagtactttt
      961 aaacaaacca ccatttcaaa atatagataa agatacaggt gaattaagag gtatatcaat
     1021 tgatttgtta aatcatatat gtgataaaac aggcttagaa tttgaacttg tgcctgtaaa
     1081 atctcaagaa gatttagaaa acatgactaa aaagcgtgaa attgatattg tagcaggaat
     1141 gatttatgat tatgaatact ctcgtgaaca taatatagtt atgtctcggc cttttctttc
     1201 tactcaatat actatgatga ttaataataa attaaatgag aatagtttag atggaaaacg
     1261 tgtagctcta gccaaaggta ttaattatta tggtcagttt gaagagaata tagtttggta
     1321 tgataattta gaagagtgta tagaagctgt taataaaggc tatgcagatt acacttatgg
     1381 cgaaggttat tctatgcagt actatatgaa tcaacctaag tataaaaatg tgcgtctagt
     1441 tcctcaaact tataaagcaa agagtatttg ttttggtata attaaaaaag ataaacaaga
     1501 attattgaat atatttaata aagctattat aagtatttca tcagaaaaaa tgcaatcaat
     1561 tgtctaccaa aatactacat atagacaaga cttctcgttg ctttatatta tgcaacagaa
     1621 tccaattgag actattttta ttgtagccag tttattaatt tgtattattg gatttatggc
     1681 atttggtttg catactcgta caagtatgaa cgagaagata tctttagaac tcaaaaaaca
     1741 tactcagttg tatgaaatga caagtgaata cttttttgag tatgattata agcaagataa
     1801 attgataatt tctatgccag aaaagggtat tgagacttat tatggtttgg ataaaaatga
     1861 taaggaaagt gaaaatggca atatagaaaa tgaaagagat aaaataagga aagaatataa
     1921 taaaaagttt tttgatgtaa taaaatcaca agaaaatgga acaactgaaa tgtattctat
     1981 tatgcttgat gatacttttc attggattag agttacttca aaaataattt ataatgatag
     2041 aaaaatacca atgtatgtaa tcgggaaaat atgtaatatt gattctgaaa aagaggaaaa
     2101 gaatagattg ttagataagg ctcaaaggga tagtttaacc aatatttata atgcagcata
     2161 tattagaaaa ttaactgcac aaaatttgtt gaatttatca actgaaaaaa atggtgcttt
     2221 attgattata gatattgatt attttaagtc gattaatgat acttatggtc acttgttagg
     2281 agataaagta cttattgatg ttgcaaaggt acttgatgaa agttttgcaa aaaatgatat
     2341 agtaggtcgt ttgggtggag atgaatttag tgtttatatc aatgatataa taaataagga
     2401 agaacttatt aataaatgta atgatgtata caatcatatt catctcatta agttgccaaa
     2461 tggagaaagt ttatctatta gtatgggggt agttatagca aagcctggtc aagagtatga
     2521 tgaactgtat caaatagcag ataatgctct ttatgattct aagagacaag gaagaaatag
     2581 atttaaaata gtataagaat taattttaaa acagaataaa gcttaataat atttaaatta
     2641 caataaagcc taatgataca tgttattatc tatcattagg ctttatttc
//
LOCUS       DAYAOX010000321         2659 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.325, whole genome shotgun sequence.
ACCESSION   DAYAOX010000321 DAYAOX010000000
VERSION     DAYAOX010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.325"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            222..1097
                     /locus_tag="ACGI57_003565"
     CDS             222..1097
                     /locus_tag="ACGI57_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HFG9718611.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            1115..2605
                     /gene="cls"
                     /locus_tag="ACGI57_003566"
     CDS             1115..2605
                     /gene="cls"
                     /locus_tag="ACGI57_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFG9718612.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
BASE COUNT         1079 a          228 c          502 g          850 t
ORIGIN      
        1 aaaaaatgaa tttcgataag tttaagtggg tataaactta tagaaattca ttttttattt
       61 aaggtaaaaa tttaaagtaa aatattattt tacataataa taaccttata aattttgaaa
      121 ttacttatct ttgtgttaaa ataagtaaaa tgcacatatt agagctaagt ttttaagtaa
      181 ataaacaaaa tgaaaatata aatattaagg agatttggta tatgaatgtt tatgaaaaag
      241 gtaatggagt tatactagaa ggagtaacag attttgaccc agtacatata tttgagtgtg
      301 gtcaatgttt tagatggcac aaacaagaag atggctcata cactggagtt gcaaagggaa
      361 gaatattaaa tgtcaaaaaa gagaatgata aaatatattt aaataatact aatttaaagg
      421 agtttaacag catttggtat aattactttg atttaggaac tgattataca gaaataaaga
      481 ataagctaaa aaatatggat gaatatttaa ataaggctac tgaatttgga tggggaataa
      541 gaatattaag acaagatgga tgggaaatgc ttatatcatt tataatttcc tctaataata
      601 ggattcctat gatacaaaga gcgatagaaa atttatcaag aaagtttggt aagtatatag
      661 gtgaatatga aggtaatgaa tactatgcat tcccaacacc agaagaacta aataaggcct
      721 cacaagagga aataagagca tgtcaaacag gttttagaga taagtacata aaaagtacta
      781 cacaggcagt tatagagaat aatgatgagg tatctgaata tacaaattta agtacagagg
      841 attgtagaaa agagctttta aaatttaatg gagtaggacc aaaagtatgt gattgtattg
      901 cattatttgg tatgcaaaaa tatgattctt ttccagttga tgtttgggtt aagagagtta
      961 tgcaagaatt ttatatagat gaagacatga gtttacctaa aatgagaact tatggaatcg
     1021 ataaatttaa agaaatgtct ggatttgctc aacagtactt attttactat gcaagagaac
     1081 tcggtatagg aaaataaatt aagggggaga aattatggga gtaataggaa caatatttct
     1141 gttctattta attatatcat atctagctgg agccataatt tctgtgatta tattgcttga
     1201 aaatagagac cctgcaaaaa caatgtcttg gctattgatg tttattatat ttccaggtgt
     1261 aggtttaatg atatatgcta tttcaggtag aaatattaga aaaagaaaat tatttaagac
     1321 tcaaaaacta gctaataata taaaagaaaa gaaactattt gatacattag aaaagataac
     1381 agaaatagtt gaattagaaa aagaatctat aaaacaaaat aagcttttaa gagatgaaga
     1441 ggatggtagt tatagaaaaa gggtaataaa tatgttgtta aagactggta tgtttccctt
     1501 tactaaaaat aataaagtgg atgtatttgt agatggaaat gaaaagttta aaagacttat
     1561 agaagatata agagaagcta aagaccatat tcatttagag tactttatca tcaaagatag
     1621 tgaaataggt agggtgttaa aagaagaact tataaaaaag gctaaagaag gcattaagat
     1681 aagaatttta tatgatgatg taggatgctg gagattttgg tttaatagaa aattttttcg
     1741 agaaatgagg gaagtaggta tagaaattgc agcattttta cctacaaaat ttcctataat
     1801 aggagggaaa ttaaactata gaaatcacag aaaaattgtt gttattgatg gaataattgg
     1861 atatacaggt ggaataaata taggagatga atatttaggc aaaaatgata agtttggata
     1921 ttggagagat actcatatta gaattaaggg tatttctgta tacatgcttc aaatgacatt
     1981 tctgattgat tggtattata cgacaaaaga agtattagtg actaaaaatt atttcccaag
     2041 tgtagggaat gttggtgaaa gtatgataca agttgttgcg agtggtccag acagtgactg
     2101 ggaagatatt cattatgctt atttttctgc aatatgtcag gctagaaaaa atgtttatat
     2161 agaaactcca tattttattc cagatgaaag tctactaaaa gctataaaga gtgctgctct
     2221 tagtggagtt gatgtgagaa taatatttcc taaaattgca gaccacaaga tagtaaatat
     2281 agcatcttat tcatattttg aagaaatatt aagagcaggt ggtaaagttt atttatataa
     2341 taaaggattt atacattcta aagtcgtaat aatagatgat aaaattgctt ctgcaggtac
     2401 agctaatatg gatttaagaa gttttatgct taactttgag gtaaatgcat ttatctatga
     2461 tgaagaagtt attagggtaa tgacagatga tttctttgag gatttaagtc actgtgaaga
     2521 acttaatttg gaggtattta agaatagaaa tatcatacaa aaaatcaaag aatctgtagc
     2581 aagattattt tcaccaatat tataaaaaat tattaaaaaa ggtattgaaa attatgtgtg
     2641 attggtatat tataataat
//
LOCUS       DAYAOX010000322         2653 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.326, whole genome shotgun sequence.
ACCESSION   DAYAOX010000322 DAYAOX010000000
VERSION     DAYAOX010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.326"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            116..1315
                     /locus_tag="ACGI57_003567"
     CDS             116..1315
                     /locus_tag="ACGI57_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718613.1"
                     /translation="MKLKSKISIFIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1324..2136
                     /locus_tag="ACGI57_003568"
     CDS             1324..2136
                     /locus_tag="ACGI57_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718614.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2147..>2653
                     /locus_tag="ACGI57_003569"
     CDS             2147..>2653
                     /locus_tag="ACGI57_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718615.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFN"
BASE COUNT         1127 a          210 c          398 g          918 t
ORIGIN      
        1 ttcaatttaa caatttagta gcagggttat taagtttttt aattataatg tttattctaa
       61 atggaatatc ttatttgcta attaaaaaga aagtatctct ttaaggaggg gtggtatgaa
      121 attaaaatct aaaatatcta tatttattat atttttgact ttagcaatat attttagtat
      181 aggtttatta aataatgaag ataatttaaa gataaaaact ataaaaggtg actctaatga
      241 attagaccat ataaacataa attttggata tgctttgaat ggatatataa attcatatta
      301 tacaataaat ggaagcaaca aaaccaaaaa gactgaatta ataaaactac aaaaggatag
      361 taagaacaca aataatttaa ttgatgtatc tagtggttca gatttaattg ctcatgttgg
      421 atttgaaatg aatgaggagt caaaagatat aaactttgtg atagatgaga aaaaagaaga
      481 tttagaaaaa ggtgtatttc catcagaagg tagtgataaa gaaattgaat atgaaaaatt
      541 agatatgaat aattttacaa ggacaagata taaagtagat aaaagtacag atgattatta
      601 tgcaaagata ctagcatcaa aaagatataa aaaatactta tatgtatctg tactttatgc
      661 tacaagtgat tcatcagaaa ataacaaaga atattcaagt actatagata taataaaaat
      721 taatccagaa aataaaaaaa tcaagactat aaaaagtata aatttaagta attatttttc
      781 cgaaaaatac caaaacatag agaacatagg tgtaagttat gctagcaaat atgaggataa
      841 gatgtatatt ttagtaaatg tactaaatac aaacataggt gataatagta attctaaatt
      901 tcatttactt gagtataatc ttgaagaaaa taaggaaagc ataatggaat taaaaactga
      961 tagcaaaatg gctttagcaa aagcaaagtt agaagctaat aaattatata tggtaatgtc
     1021 gaatgattat gataatagtt caattacaga gttaaattta cttacctata atatattaga
     1081 taaaagctta gatgaagata aatatgtttt agataataga gaaaaaagag gaattagaag
     1141 tattgaaatt gattcaaata aaatcgttgc atatacatca aggctaaact ctatattatc
     1201 ttgggatgat aaatttatgc tatatataat aaataaaaat tctaaggaaa ttgtatatga
     1261 aggagaatta caatttaaca gttcaaatag tatgttagaa tttgaaataa agtaggtgag
     1321 ttgatgaaag atttaataag attattcgaa tttgaattaa aaagaaatat aaagaattat
     1381 atatttataa taatttgttg ttgttctttt gtaattttaa acatagtaaa aaatttaaat
     1441 gactataatt acataataga aaatgcagtt aaaagtgaac agttaacaaa aatcggaaat
     1501 acgatagaaa cggtaaatgt tacaggtttc tcaagcttta gaaatataat gggttctaca
     1561 gaatcgtggt ttatgtttgg tataattgca tgtatttgct atgctttttt tatttggtat
     1621 agagacttta atggtagaag taaatctata tatacactta taatgctacc taaaaataga
     1681 atcaatattt atatatctaa attattaaat atattatttc tagtgtatag ctatacagta
     1741 gtacttacaa tatctttgtt tattgcaagt aagttgttgc ctagacatat gcttggtaat
     1801 gtcacaaact atggttttgt acaagaaaca atatatgagt taaaaatgtt acttccatat
     1861 agctttgaaa tactatttgt agagtatata tttttattga taggatttgt atctgtagta
     1921 tttacatcaa ttcttataaa taaatctatt tataaagtaa gcactttaat aagttttcta
     1981 tttttagtta tagaaatttt agtgtttata atttctatag aatttattat accttacgaa
     2041 tacagtgatg tatttactgt tctatattca agtataaaca tatttgtatg tagtcttata
     2101 tctaataaac ttttgaagca aaagattgac ttttaggagg gatattatga aagcaaaaaa
     2161 tataggaaaa tatacaatat gttttataat agtatgtttt ttaatgatta ctatgacctt
     2221 aatattgagt tattttaata aggataaact atctgtaaaa actttaaaag gcaatgtaaa
     2281 agatataggg agagtaagtg tagtatatac acctaatata agcaaatttt cttatgagga
     2341 ggtaattata agtaaaaatg gagtaagaaa taatacaaac ccaaaatcaa gaattagaaa
     2401 tgattattta aatattgaca aaaaatacag tgactttgtt aatgataaag gaaaagaatt
     2461 tatatataaa gatgatttga atataggaga agcgtatacc tctgttaagt ctgatttagt
     2521 acaaggaatg aatgctttaa atgattgtat aaaggtttca aataaaaatt taaaaagtgg
     2581 ggtagaagaa aattttgata ttaatttaaa taagtctgat tttaaagaaa atagttggaa
     2641 aaccttattt aac
//
LOCUS       DAYAOX010000323         2633 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.327, whole genome shotgun sequence.
ACCESSION   DAYAOX010000323 DAYAOX010000000
VERSION     DAYAOX010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.327"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            128..2407
                     /locus_tag="ACGI57_003570"
     CDS             128..2407
                     /locus_tag="ACGI57_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9718616.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
BASE COUNT         1064 a          259 c          428 g          882 t
ORIGIN      
        1 aataaagttt ataattttgt ataattacag taaatatgga aaaataagta ttattataaa
       61 atgtttacaa taattgtcat aagataatga ttatacatac atagattcga gaaaggcagg
      121 aagcataatg ttaagtgatg caaaaaataa taggtatggt ttgtccataa ttgctgtcat
      181 aagtatctta gtaattacaa tttcctgttt gatttatggt tcaaggttga ataagacatt
      241 aggtgaagaa acgaatcaat atctttcaga aatagcaaat caaagcgtca atgtgttaaa
      301 aaaacaaatt aatggagata taaagttact agagtcaatt tcaatattta ttgaaggaga
      361 agagtctttt gaagtagata atatcctttc tatactaaaa agacaagcta ttaatagttc
      421 atttaaaaga atgggagtta ttttaccaaa tggtacagca tacactacag atggttatat
      481 agaagacttc tctaaaagag attatttctt aaaatcaatg caaggagaag tggttataac
      541 aggaaaatta aaagatgtaa tagaagatga taaaaatacc aatagtaata ttaatgtata
      601 ttctgtacca atttataaag ataatgaggt taaaggagtt atatttgcca cacatagtac
      661 gaagttgtat gaaaaaatac tttctgtacc tacatttaat ggaaaaggat attcttatat
      721 aactagaagt aatggtgata ttgttttaaa tccaagcagt aaaaacgcca actctactaa
      781 agaaaattta ttttcttaca ttggcagaag tgattcaata tcaaaatcta gtatcaacaa
      841 tatgaagtca aatataaaaa actttaaatc aggttcatta agttatagta tgtttggaaa
      901 aggatattat ttaagttatg ccccaatagg aatcaatgat tggtatcttt tttctgaagt
      961 tcctaaaact gctatatctg agaaatcata tgctattata aaacttacat tatcagcatg
     1021 tgtagtcttg attataatct ttacggcatt aacaatttat atactattta tacataaaaa
     1081 gagtaaggaa aaattggaag aatttgcttt taaagataac gttacaggta ttggcaattc
     1141 aaataaattt aatcttgaag ggggaaaatt tttatcctct catgagaaaa agaatctagt
     1201 tttaatttat tttgatatag ataagtttaa gctagtaaat gataggtttg ggtatgaaga
     1261 aggagataga gtattaaagg aaatagcaga gataattaag aatatattta aagagcaatc
     1321 tgtattttca agaatttcaa atgacaattt tgctataata tttgaaaaga aaaataatag
     1381 agagtcaatt atagaaatat gtgaacttat acgtaaaaaa ttgtcaatga taaaaacaag
     1441 tttgggagta gaactcaatt taattccatc cataggtgta tattttgttg aagagggaga
     1501 aacaaatatt tctacctgtc ttgacaaagc tatgattgca aaaactacag taaaaagaaa
     1561 atatttaaaa gtttatgaga tttatgctga aaatttaaaa gaaactctca tagaagaacg
     1621 tgatattgaa caagaaatgc atgatgcttt aaaaaatgaa caatttaaag tttatttaca
     1681 gcctaaggtt gatttatcaa ctactaaaat tgtaggtgct gaagcacttg tacgttggca
     1741 acatcccaaa aaaggtctaa taagtccagg tgtatttatt cctatttttg agaaaaatgg
     1801 ttttataaca gaattggata tgtttgtatt tacccaagtt tgtaaaaatt ttaaacgttg
     1861 ggaaaatgaa aatttcccca catttcctat atcaataaac ttatcaagag tacatcttga
     1921 gaatccaggc tttatttctg agcttgaaag aataacaaaa gaatatgaaa ttgaaccaaa
     1981 tttaattgag attgaattga cagaaagtgc aatatttgac aatacaaaaa tattatttaa
     2041 aataatgcaa actttgaaat ctgttggatt taaaatttct atggatgatt ttggttctgg
     2101 ttattcttct ttaaatatgc ttaaagatat gccaattgat gtattgaaat tagataggca
     2161 attctttata acagtgggaa atgcaaaaaa gagtcaaatt gtagtttcaa gtattgttca
     2221 aatggcaaag caacttgata taaaagttgt ttcagaaggt gttgaaactg tagagcaagc
     2281 agagttttta aggtttatag gttgtgatat ggcccaagga tttttatttg ctagaccaat
     2341 gccaatagaa gaatatgaaa aattaatatt agatagatat agtttaaata gaaaagaaac
     2401 aaattaaata aaattgtgaa ttataacata aaaatttata aaaaagtgaa ctttgttaat
     2461 caatgttcac ttttttatgt aaaaatatac tattataaaa acataaatca ttgttaggat
     2521 attattttag gaattattgg gcaaagttta ttaatatgta gtatttttta catttgaatg
     2581 tttttgaggt aagagtatat gggattatag caaaatactt tagatagtaa taa
//
LOCUS       DAYAOX010000324         2614 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.328, whole genome shotgun sequence.
ACCESSION   DAYAOX010000324 DAYAOX010000000
VERSION     DAYAOX010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.328"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(105..1559)
                     /locus_tag="ACGI57_003571"
     CDS             complement(105..1559)
                     /locus_tag="ACGI57_003571"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFG9718617.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            1723..2592
                     /locus_tag="ACGI57_003572"
     CDS             1723..2592
                     /locus_tag="ACGI57_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFG9718618.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
BASE COUNT          899 a          397 c          301 g         1017 t
ORIGIN      
        1 ttattgcatt atctatttta caattattta catataaaaa aatgtaatat caaagtgaag
       61 cttgatatta catttttgtt cttgtatttt gtttaatttt tatgctacat cttacttaaa
      121 cactcatttg caacttcttt atcatcaaaa tgatgcttaa ctttacctat aatttgataa
      181 gtttcatgtc ccttacctgt tataattaca acatcatcct tttttgccat acttatagct
      241 tctgctatag cttgttctct atcaacaaca acatggtaat tttctttttt cttgtcaatt
      301 ccttcaagta tatcttttat tattgcctct ggttcttcag ttcttggatt atcagaagtt
      361 ataatacata aatcagaata tttttgtcca attgctccca tcaaaggtct cttttcagta
      421 tctctatctc caccacaccc aaacacagaa attatttttc cttctgcaaa ttgttgagtt
      481 gtctttagta cattttcaag tgcgtcagga gtatgagcat agtctacaat tacgcttata
      541 cctttatcat ttggaactgt ttcaaacctt cctgatactc cattagaaag tctaaggcct
      601 tctttatata ttggtttagg tatgccaagt acatagcaag cagctataac tgctaaagta
      661 ttatatactg taaacatccc tggaactggt ataaatatta cttcttcata tgaaggtgtt
      721 attaatctat aagatactcc atccgaatct gattttatat ctctagccat aaaatcagcc
      781 ttagtatcaa caccataagt gtaacaaggt acatttatac cttttatatt ttcatatatc
      841 ttttttccac cttcatcatc tatgtttatt atatttgcca tagtagtctt aaaaaataat
      901 ttttcttttg cttttctata atcctctaaa tctttatgaa aatctaaatg gtctggtgtt
      961 aaatttgtaa atatgcctag tttaaagtct gtttcatcaa ctctatttaa tgccaatgaa
     1021 tgtgatgaaa cttccattgc acagtagtca catccattat ccaacatatt attaaaatga
     1081 tattgtaaat ctatactttc tggagttgtt gaattggaaa caatgtcttt ttctccatca
     1141 aaaattttta tagttcctat aagtccaact ttattcttat ttaaagttaa tatctcattt
     1201 agtatagtag ttatactagt ttttccatta gtacctgtaa ctcctataac gttaaatttt
     1261 tgagatggat gattgtaaaa attatcagct acttttgcca tatcttttct tgtatctttt
     1321 acctttataa atgtacatcc ttttattgct acatcttctt caacaagaaa tgcacttgct
     1381 cccttttcta tggcatcttt tatatactta tgaccatctg acacaaatcc ttttatacat
     1441 ataaataatg taccttttgt aacctttctt gaatcatatt gtacattatt aatatctata
     1501 tttaattcac ccttgacatt gattatatcc aacccctgta taatatcatt taatttcata
     1561 aatgctttcc tccctttaaa ctctttctgc taaaattaca ctacatataa tattatatta
     1621 agaatttttt actatgtgaa tggttaaaaa tgcttgaaac attgcaaaaa atgctctata
     1681 attttctata atataactga ggaaaggtct tctataaaaa ctatggataa taaaatcggg
     1741 tacttaaacg ataactttaa aatatttaat attagagata aaaaagatat acaatttgaa
     1801 taccataatc atgattttaa taagataatt atatttataa atggaaatgt aacttatttt
     1861 gttgatggta ttccctataa actaaagcct tgggatatac tctttatcag caataatgaa
     1921 atccataaac cagttataga ttcaaatgtc ttttatgaga gaattgcaat atggattagc
     1981 ccagaattta tgaaaaaaaa tagtgatgct agtagtgact tagaaaaatg ttttaaaata
     2041 gctacagaga ataaatgcaa tagattgcgt ttaaatatgc atgatattga atctattaag
     2101 agttttattg agcaaataaa tatttccgaa tccaatactg aatttggaaa tgaaatattg
     2161 agaaatacat tatttataca acttatgata ttcttaaaca gaattttcct aaaaaatgaa
     2221 aatatggaat caacagaaga tgttttttat gatgaaacta ttaagaaagt attaaactat
     2281 ataaattcaa atatagatga taagctttca atagatagca tatcgtctga attttttata
     2341 agtaagtatt atcttatgag aaaatttaaa tctcatactg gaacgtctat acacaattat
     2401 atagttcaaa aaagattaat tttggcaaag tcactaataa ccaatggtca ccttatgagt
     2461 gaagtttgta ataaatgtgg attcaacgat tattctagtt ttgtaagggc atttaaaaaa
     2521 aactacggtg tatctcctaa aaattatata aacaataaaa atcctttaga tattactctt
     2581 ttagatgaat agataattta tgtaaatttt attt
//
LOCUS       DAYAOX010000325         2556 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.329, whole genome shotgun sequence.
ACCESSION   DAYAOX010000325 DAYAOX010000000
VERSION     DAYAOX010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.329"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            467..946
                     /locus_tag="ACGI57_003573"
     CDS             467..946
                     /locus_tag="ACGI57_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9718619.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            943..1722
                     /locus_tag="ACGI57_003574"
     CDS             943..1722
                     /locus_tag="ACGI57_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718620.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFIPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1972..2472)
                     /locus_tag="ACGI57_003575"
     CDS             complement(1972..2472)
                     /locus_tag="ACGI57_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFG9718621.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT         1002 a          351 c          315 g          888 t
ORIGIN      
        1 gttcatctct atctaaaata atgacatttc taactcttaa ttctcattta agtttcattg
       61 ttttttctta aaagcctact tttagtcaag atactgttat ctttactcat tatttaaaga
      121 acttatttta aatctgtaca tgaagtaaat aatccaaaag cattgttatt ctaactactc
      181 aacattaaat acttactatt ttctaattac tcaatataaa gacttactat aaaactaaat
      241 acataaatgg cttctaaagt ttaatatatc tacatggctt tatatatgac aggaaaaata
      301 ctagaaaaaa tatattatag aatacttgta tataataggc aaaactttca aacttaaatt
      361 tatcaaataa tcataaaaaa agtgaaccat ctatagtaaa attttatcta ataagtgaaa
      421 ggcaaaaaag tagcgctacg atttgcatag aagggaagga acttagttgg ataaaactac
      481 atttacaaac aatatacttg aatcagaaca gactttgtat cgagtatcta agtctattct
      541 aggaaatgac caagactgtg aagatgccgt caacaatgct attttaaaag catatgaaaa
      601 gttggattct cttaaggaag aacaatactt taaaacatgg ctgattagaa tagtcataaa
      661 tgaatgtaat tcattaagac gcaaacgatt aaaatctcta tcatttgaag atgtcttcaa
      721 aaacaaaaaa attgatgaaa aagatgacta tagcgatttg tatactgcta ttcaaagtct
      781 acctaaaaaa attaaaattc caatagtcct ttactatatt gaaggttatt cagtagatga
      841 agttaaggaa atattagata tacctcaagg aactgtaaaa agtagattat caagaggaag
      901 aagattatta aaaactaaat tagaaaacac agaggtaatc atatgaataa aaatatatta
      961 aataattcgt ttccagaagt tccagaaagt tttcataata gtttaagcaa aacactaaat
     1021 agtttaccag aaagagagga aaattataaa atgattaata ataaaacaca caaattacca
     1081 tttagaaaag gcttagttgc aactttagcc attactttga tattgagcac aacagccctt
     1141 gcagtaggac atatatatag tttagttgga aaatcaagta ctaaggctac ctatacatct
     1201 atcccaacta cagaacaagt aaaaaaagac tttaacttta cccctaatat agtttctgaa
     1261 tttagtaatg gatataaatt taaaggagca tatactgtaa ataaaaaagc atttgacaaa
     1321 gaaaataatg ttttaggaaa atcaaaagca cttagttttg aatataaaaa tggcaatgat
     1381 gaaatatctc ttgacacagc aaataatgta cttgggaaga gttctgacaa tctaaaagtt
     1441 gtagatactt acaaagatat taatatttct tatgaagcat tcgcacaaaa gtttatacca
     1501 gaaggttata aaatgacaga acaggataaa attgatgaaa aatcaggcaa atacacattt
     1561 agtgtggggc caaataatga atcagacaaa gttgaagtta atgaatttaa gtttttgaca
     1621 tggaaacaag atggagttta ctattctttt acagttcaag actcaaattt atctatgaat
     1681 gacttagtta aaatggctca tgaagtaata gatgcaaaat aaacataagt aataataagt
     1741 tgtttatgag tttattattt atgactttaa tttttttatt agtaaacatg gttgtatctt
     1801 ccatctttat attttcttta aatttaatat ataaaatttt agatatttag ctaccttcaa
     1861 aagaagcatt attaaaaagc ccttaaaaat atttctttta tatggaattt taagggcttt
     1921 aactttattt tatacaagtg tatatggaat tttaaagact ttaactttat tttatataag
     1981 tgtaagctcc atttgaatta cttcttcttt tacaggtcta tcattccatg taaaaccact
     2041 agatattgta tttacctcta caaaccctaa agatttataa acatgaatag ctctactatt
     2101 acttttaaat acatcaagct caattttttc atacccatat tcttttgata aatcaataat
     2161 ttgttttaaa agactacttc cgataccttt ttccctatac tctttaagaa tacccatgcc
     2221 taaataacca actttttcag ttttaggtaa aacgtcacac caaccaatac agttatctga
     2281 ttctaaatca attataaaca gttgtggtaa gtttttattt ataatatttt ttataaattc
     2341 aactgtttct tcaaaaggaa aggcttcatc catagctaga tatttcctct cttttgctat
     2401 tttatcaaaa gtttgccaat acgaataaat atacttttct tctgccttta taaattttat
     2461 gccttctatc aacttaacat ttcccctttt taatttttga ttgtagttaa cttattttat
     2521 atgattctag tttataatgc tatataaatt actata
//
LOCUS       DAYAOX010000326         2549 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.330, whole genome shotgun sequence.
ACCESSION   DAYAOX010000326 DAYAOX010000000
VERSION     DAYAOX010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.330"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            439..1314
                     /locus_tag="ACGI57_003576"
     CDS             439..1314
                     /locus_tag="ACGI57_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9718622.1"
                     /translation="MLVYYLYNLIFLEGGVITLKLKIVGSGGMSIIPSSFCKCKICEE
                     ARQKGGRYERLGPSLYIDDIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGI
                     RIVEKIGCDFITGENNPIGFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKI
                     NNFDIDLINNNPDRNITFYVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDD
                     GILNDGTKLKDTPFKDEIFTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKK
                     LDNIYFAYDGMEIIV"
     gene            complement(1624..2403)
                     /locus_tag="ACGI57_003577"
     CDS             complement(1624..2403)
                     /locus_tag="ACGI57_003577"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFG9718623.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT          933 a          373 c          296 g          947 t
ORIGIN      
        1 taagattata tactatcctt atactctagt taaatatatt ttaatcacta atataaatta
       61 gtttttttaa caaaacaata aaagagtaaa gtacaaataa ttcctttgta ctctactctt
      121 tttaactttc tttgtacaat aatacaatga taaatataaa aaattatata ttccattcta
      181 tagaaatatg ctcaccaata acatgtatgt tgttaatcaa tatattatat caaattatat
      241 caaaattaca taaaccttat aggacaatca tatcttatta caagaaaaat gtcttatcca
      301 tattgaataa gtcattcagt tcatatacaa agtcaatttc attgagaaac atttaatata
      361 tgtttataaa aatttcatac tattctttca tatttacatg tgatataatt tattaactca
      421 taattttgtt aatcagttgt gcttgtatat tatttatata atctaatatt tttagaaggt
      481 ggtgtaataa ctttgaaatt aaaaattgtt ggtagtggcg gtatgtcaat tattccaagt
      541 tcattttgta aatgcaaaat atgtgaagaa gccaggcaaa aaggcggaag atatgaacga
      601 ttaggaccaa gtttatatat tgatgatata aaaatgctga tagacacacc tgaagacatt
      661 gcagtagctt gtaacagaca aaaaatatcg gaagtaaaac atatttcaat ttctcaccat
      721 gacccagacc atgttaaggg tattagaatt gtcgaaaaaa taggatgtga ctttatcaca
      781 ggagaaaata atccaattgg cttttatgcg cttcctgaag taattgaaga tataaaccga
      841 acgaatctcg attgtcttaa atattatggt gatgttataa attgcatttc tattaatgaa
      901 acttcgcatg taaagataaa taattttgat attgatttga ttaacaataa cccagacagg
      961 aatataacct tctatgttat caaagagaat gaaaaaaaag tcatatatgc ctgctgtaat
     1021 cccaagccat ttactcatag tgatatgtac tttgatgctg atattttaat aataagtcta
     1081 gtttctgatg atggtatatt aaatgatggt acaaaactta aagacacacc atttaaagat
     1141 gaaatattta ctttagatga gattgttgaa attaaaaact attatagaat taaaaaaatt
     1201 attgttacac atattgacga aatgtgggga aaatcatatg gttattacag tgaattagaa
     1261 aagaaacttg ataacattta ttttgcatat gatggtatgg aaattatagt ctaaatcatt
     1321 aagtaataag cataatgcaa aataagaaaa cttcaattta acagtatagt caaactatgt
     1381 gatatcaaca tatcgcagtt tttacaaaat atcatagtat ttatctttat aaaatttcaa
     1441 gtcctacata ttttaatact tcaatatcta aataaagtaa aatcaaaaat atattttata
     1501 tacattatag acggcttatc gtcactagat aagccgtttt gtcacaatta ttatttgctc
     1561 aacaaaactt ctcataatat ataacagatt gaatctccaa ctgatactta attaccaaaa
     1621 ctcttattcc ttaatatgtt gttgcattaa ccaaaccttc atcatcattt tttgtggcaa
     1681 tagttttgct aataaatgac tggattttgt atttatacca tacacagaaa tatccttatt
     1741 tttctttgca tccttcaatg cttttttggc aacaacgtct ggagacacta tgcctgggaa
     1801 attttttgtc cctttttcag ctacaactat acctctctta aatagatttg tactcatcca
     1861 acctggacat acagcaattg catttatccc tttactctta agttcaacat ttaatgctct
     1921 tgtataattt cttataaacg ctttagttga actataaata ttttgatatg gcaatggctg
     1981 gaatgatgct tgtgaagaaa tattcatgat atggcttccc ttttgcatat atggaataca
     2041 tataagtccc aatgctacaa cagcactaat atttaaatca atcatattaa tagattcatc
     2101 tatacttaaa tcattgtatg aacagaattt tgcaaaacca gcattgttta taaggtattt
     2161 tatacataca ttttctttac ttaaaaatgt accaatttct tttacttctt ctatttttga
     2221 taaatctttt gaaaaaattt ttaccttact tccaaattca tctactaatt gatttaatct
     2281 cgccttgttt cttgcaactg cccatatttc tgttatttct tcttcattta ttaataactt
     2341 tagaaactct ttaccaaaac cactattagc acccgttata attgcaatct tcttataact
     2401 cataattatt ctcctttacc ataagtttat acgctcaaac tgtaaactta ttttttacaa
     2461 ataaaacacc ctctattcag atgttatatt catattattt gagatattat atttatatca
     2521 ttttcaataa ttgttatata gcataaaaa
//
LOCUS       DAYAOX010000327         2481 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.332, whole genome shotgun sequence.
ACCESSION   DAYAOX010000327 DAYAOX010000000
VERSION     DAYAOX010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.332"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            1029..2384
                     /locus_tag="ACGI57_003578"
     CDS             1029..2384
                     /locus_tag="ACGI57_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFG9718624.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT          883 a          263 c          410 g          925 t
ORIGIN      
        1 aaatttaatt taaatttttt attctattaa ttattaatat tattttcaaa tcaatgcagt
       61 tataagaagt gtataatttg attattatat actgcataca aaaatatatt tgagcaaata
      121 agtaaagtaa taaaaaaatc aagatttcat atctaaaaac tattctgaac attaatttaa
      181 tttatatttt cataggaaat attttatata atataattga taaatataat ttgaattttt
      241 ctaattattt actgtcgatt aatagatttt tttcttgcat ataagtgcat atttatggta
      301 cgatttacat ggtgaaataa aaaaggggag gtgaaaaata aatattcgtg aaaaagtttt
      361 cagtttgagg tattttatga ataatatatt ttgaggtatt ttataaataa tatattatac
      421 ataaagagta tagcctcaat aaatagtatc aaagcgaata tatgggtaat tactttaaaa
      481 taacagtttt tatgtttaga ttcacagtgt ttattataaa ctaatgtgta agaaataagc
      541 ctaatctgtg aatagatagt agtttagtta tttaagttga gaattggttg ggtgaagtaa
      601 tatataaatt taaaaaataa ataaaaacat tatttgaaca aaatatatgt tgctttattc
      661 gagactcctt taaaaaagca ctaaaatgta attacataag ttgctacaaa agggggattt
      721 tagtttgtaa aatactatca cgtaaaaaat aagaattgca tatggcgaag gttttttcat
      781 ggtattagat gagaatatat taatttataa ctatatctga aaatagtgaa tttatataat
      841 ttaaatttat gattaagaag tgtattaatt ttattccatg agaatatgta ctctacaaat
      901 aaatgcgcaa ttatcttaat aataattaca aattgtttta caaaataata atattatatc
      961 tttcataaaa gaaagaattt ggacaaaacg taaaaaataa aaatacaatt aagaaaggat
     1021 ttaaaattat gagctcaaca gtaaaaaaga agggtaaatt tcctatggga ttttatatct
     1081 gctcaacaac tttctcattt gaaagagcag catattatgc ttcaaagtac ttaatatata
     1141 tattccttac aacagctata gttcatggtg gtcttggaat agataaagga caagctgcta
     1201 taatgcaagc caaccttgtt gcattcacat accttgcacc tataattggg gggtatatat
     1261 cagatagatg gataggagct agatatacta tacctatagg tatgcttatc atgggggtag
     1321 gatactatct tggttctatt gcaactacag tttctatggt aaatgcaatg ataatcttag
     1381 tttcaattgg tactgcattc tttaaaggta acgtatctgc ggtaaatgga cagctttttg
     1441 atagtcaaga agaacttgat acagcatttt cagtacaata ttcttttgtt aatataggtt
     1501 cttttattgg tactatagca gttggtattc tttaccttaa aacatttgca aaaaatggtg
     1561 tacttggatt ttctcaatgt ttctttatag cagctgtact ttgtgtaata ggtgcaatat
     1621 ggttcatata tggatggaga ttccttggga atgcaggaaa gagacctttt aaagaaggtg
     1681 tagttgctga aaaaattgaa gaaaaagata agtctcctct tacaagcatg gataagaaga
     1741 gaatttgggc tattatactt atatcattct tctctgtaat tttctgggta ttctggtatc
     1801 taacttatct tgcagtttat gattatggtg cagcatttgt aaacatgaat gttggaggat
     1861 ttgatgttcc tcttgcttgg tttgactcat taaattcact tgtatgtata gtattaggac
     1921 cagttcttgg agcattatgg tttaaacttg ctagtagacc acaaggagat atgagtttat
     1981 ttaaaaagac aggtcttgga ttaatattct taggtcttgc attcttaatg cttgttggtg
     2041 cagaattttc aagaggagtt ggagctcctg aaactgctaa agctagtata ctatggataa
     2101 tcatgtttgg tattctttta agtcttggtg aaatgttatt ctcaccactt ggtaactctt
     2161 ttgtaagtaa gtatgcacct aagaagcttc taggtgttct tatgggagta tggacatttg
     2221 ctacttttat agctggtaaa ggatatggat atatatatgc atttactctt aaatttgata
     2281 tgattaaagt atatactata attccagtaa tactatttgt tgcagcgata ttattattct
     2341 tatctgataa gacattaagt agacttgttg aggacgataa ataatcagta agcaattatt
     2401 atgttttata ggttaatcta ctgtaattat gttgtattag attgatttat aactaattaa
     2461 taataaaaat aaaattttac t
//
LOCUS       DAYAOX010000328         2451 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.333, whole genome shotgun sequence.
ACCESSION   DAYAOX010000328 DAYAOX010000000
VERSION     DAYAOX010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.333"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(181..1473)
                     /gene="brnQ"
                     /locus_tag="ACGI57_003579"
     CDS             complement(181..1473)
                     /gene="brnQ"
                     /locus_tag="ACGI57_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFG9718625.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          825 a          392 c          253 g          981 t
ORIGIN      
        1 aatctttttt attttattat ctttataaat catactactt caaaaataaa tttttaaatt
       61 tatcaaacag gattaataat gttcttttat ctacaataat ttaataattc ttttaaatat
      121 aaaaaggaat tgcctatata atttaaaggc aattccataa ttatcaatta gtttttatta
      181 ttatgcagtt ttactgcttt tagatggaat aaagtttcct attattcctg ctattacaac
      241 tggaactatc cagttaaatc ctaatgaagc aaatggtaag ctattaacta gaggaatttt
      301 tacaccaaaa cttgttgcaa ctgttaatac acttactaat aatgccatgt atgcagcaca
      361 tttgaacacg ttgtcatttt taattttatt actaaataat gctagtataa ctagagttat
      421 agttgctgga tataccatac ttagtatagg agaagagaat tgtattattg tacttactcc
      481 aaagtttgat atgattgcac taaatacaca aactactata actatatgct catactttaa
      541 ttttccatct gtaagtgttg agaaatattg accagctgct gaagaaagac ctatagctgt
      601 tgttaaacat gctaatgcaa ctataatagc caataaaatt ttacctgtag ttccaagtaa
      661 agctgctgtt attgcaacta ttaaagctgt ttgttgaaca tcttgtccat attgttgaga
      721 cacagttgct ccaagataac ataatccacc ataaactaat gccattccta ttactgcaat
      781 tacacctgca cttattgtgg ctttcatctt ttctttctca cctgtataac ctttagctat
      841 tactgacatg attataattg atgcaaatac actagctgcc aatgcatcca ttgtttgata
      901 accttgagtt ataccttctg caaatacatt ttgtattaaa gaactgtctc ttatttctcc
      961 taatggagat acgatacctt taactattaa tactgctaaa gctataagta aagctggagt
     1021 taatattgac ccaactatat ctacaacttt tgatggtctt attgttaaaa ccaatactag
     1081 agcaaagaat actatagaga atattactgg gcttattcca gaacctattg taggtaatat
     1141 acccatttca taagttgttg ctgctgttct aggtattgct aaaagtggcc ctatacagat
     1201 taccattgca catcctaatg caatacttaa tcctttgcct gcccttgaga acatcttcga
     1261 tgtgtctcca ttgaacttag ttgctgctat aactgctaat aatgccaaac caccatctgc
     1321 aaaaaggaat cctatgaaag ctactagcca actacttcca gataaaactc ctagatatgg
     1381 agggaagatt aagttccctg caccaaaaaa cattgcaaat agtgcaaatc caattacaat
     1441 tacatcttta aaattgattt gtttctcacc catttaaagc acccccattt gaattttact
     1501 accttttact aaaaaataag tccaatctct taattaaatg aaaacctttt gacgagattt
     1561 tatttaatta tacatttttg acttaaattt gtcaattatg tttcataatt tataaaatct
     1621 tcacaatatt taatacaact ttcttaatta aacaaaatca acctgtatac tttaaacata
     1681 gttggttttt tgttttttct ctaatttttc ttgataaaat taactattcc gaataattaa
     1741 aatattaaga ctttttagat tattcgattt attattaagt tgtattgctt tatatactgt
     1801 atatatcact ctcctttttt acattcatat aatcttttta attcaattta aattttaaat
     1861 attttgtgtt tattatataa tctgaaaaaa aagcagtcaa tagcattttt aattttttta
     1921 attttcaaaa ttatgattct taaaaaatcg ttttcctttt atcataacac ctatttacgt
     1981 taattcttgt agtattttgt atttattttg ctaaattgac agttaacttt attcaatttt
     2041 tattctactt gcgtagattt gcctcttgtt ttttactctt aaaaccattt tttctgaaaa
     2101 ttccttatta tgatttatca acttattttt cttcataaca attactctct aacttccaac
     2161 ttacttaaac tttgcagttg ctagtattca cgtttacata cttatatgtc ataattaact
     2221 aaaaattctt aatatatctc tcaatgaaaa caatttactc atttatttaa tacatttata
     2281 taatcatatg tcttaaattt tgttttatta ttttattatt gtcacaattt tatttatatt
     2341 atatttttgt aaaaatcatt taatatgtat acttaaattt ataaaattac tctaaaattc
     2401 ttttttaatc tctattattg ttaaaaaata aaccatatat ttttttgata t
//
LOCUS       DAYAOX010000329         2418 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.334, whole genome shotgun sequence.
ACCESSION   DAYAOX010000329 DAYAOX010000000
VERSION     DAYAOX010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.334"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            38..436
                     /locus_tag="ACGI57_003580"
     CDS             38..436
                     /locus_tag="ACGI57_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9718626.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVK
                     INLNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            424..1362
                     /locus_tag="ACGI57_003581"
     CDS             424..1362
                     /locus_tag="ACGI57_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFG9718627.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1746..1970)
                     /gene="secG"
                     /locus_tag="ACGI57_003582"
     CDS             complement(1746..1970)
                     /gene="secG"
                     /locus_tag="ACGI57_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFG9718628.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
BASE COUNT          919 a          317 c          274 g          908 t
ORIGIN      
        1 aatttaacta atttatataa aatcttaagg agttgccatg ataaaaaaaa tagaacatgt
       61 ggtcattact aataataata tagaaaatta tatcaatttt tataaaaaat ttggtttttc
      121 atctaaagaa atgagcaaaa attataaact taatgctaga aatgttaaga taaatcttaa
      181 cataaaaggt tataaatttt taccttatgt taaaaattca aaagcaagta gagataactt
      241 atgttttaaa ataaacacaa atattgatga gccaaaaaaa tacttagaac aaaatgagat
      301 tcaaattaag agaggaattg tagaatgtac aggtgtcttt ggtaatatga aatctattta
      361 tttgaaaaaa actaatggaa atttaattga attatcaaat catagaatca cggagagtaa
      421 accttggaaa atttaatcct atcaataaat gttgttcttc ctttgtttct cacaatgtca
      481 cttggatatg ttctaaaaaa attaggtcta tttgatactc atactttaaa taaaatgaat
      541 aatgtttgct tcaagtcttt cttgccatta ttattatttt ttaatatata caatacagat
      601 ttagatggtt ctcttaattt taagcttatg gctgtagctg ttataattat tttttcaatc
      661 ttcttaatct tagtctttat tattcctaaa attgaaaaag ataataagac aaggggtgca
      721 ttaatacaag gtgtatttcg tagcaatttc gttatatttg gtattcctgt gacaatttca
      781 ctatttggcg ataagagtat tggagtcaca tctttattaa ttgcagttat tgtacctatg
      841 tttaatgtat tatctgttgt aattcttgaa atttttagag gtggcaaagt aaatataaaa
      901 catatgatta aaagcgtctt aacaaatcca ctcataatcg cctcttttat aggattgaca
      961 atgctaattt taaatattaa tatacctaca tttttacaca aaactattgg tgatatttct
     1021 aaaatagcta ctcctttatc acttataatt ctaggcggct cttttagttt ttctgctata
     1081 aaggggcata taaaacccat tatattgggc gtatgtggaa aacttatact tgtacctgct
     1141 atatttttac caattagcat ttttcttgga tttagaaatg tagaattagc ttcacttatg
     1201 ataatgttat ctgctccaac tgcagtttct tcatttacaa tggcacaaca gatggattct
     1261 gacagtgaat tagctggaca aattgtagta tttacttctg gattttctat aattacagta
     1321 tttttaataa tatttatttt aaagcagaat aatcttattt aatgatattt gataacacaa
     1381 attggtatta taagaaacat atataaaact gatttaatcg cgtaaaaaca aattattata
     1441 ctatttttac tatataatat aactttgtgt atattatctc attaaacatg gtgaaagtag
     1501 tgttgctata tattttttag tttgaagaaa atatataaat acaaacaata tataaataca
     1561 aataataaac gccaccaaat agtcaatcta taaaataaaa actggactaa tcatataaaa
     1621 tcattagtcc agcttttatt ttacgtactt tttatgttga tgtactttta ttaaaaacta
     1681 aatttaattt ttaaatgtct agcaaatgca ctaattaaat aatggccagt tattcaacaa
     1741 tatcactact tcttaactat aagtaatgcc aatgcattta taaaaaataa tactgctgtt
     1801 atctttgtaa ctctagcaag aaatgcttgt tttccttttg gtttaaagta agcttgactt
     1861 ccttctccac caaattctga aggtaaagca ttcttagaat cttgaggcat tatgctaatt
     1921 actagtaaaa tccctagtac tacttgcaca cccattaaaa tgttgctcat tcttgcccct
     1981 cctatctgtt tattacttaa atttcattac tctttagtgt atcacaataa tatagccatt
     2041 tcaagactat tatgctataa ataacataca aaaaaatata ttgttactat aatatattac
     2101 tatattctca atataaaaat gaataaaact tagaagagaa aactatttaa attaatacat
     2161 aaattaacac ttaaaataat ctactctcat tgtatatatt ataaatagcc accaaaataa
     2221 aacaatcatt ttaaaatatt gatataactt ttaatttcaa cataatttaa aataaataat
     2281 ccacatttag caataatact tcaatgacaa ttatgattaa atcaaaatat gtcttcactt
     2341 ttatttttta ataaaaacct cctatgcaat tagataaaaa tgagatagaa acatttattc
     2401 atattttttc tgaatcta
//
LOCUS       DAYAOX010000330         2408 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.335, whole genome shotgun sequence.
ACCESSION   DAYAOX010000330 DAYAOX010000000
VERSION     DAYAOX010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.335"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            194..2266
                     /gene="ptsG"
                     /locus_tag="ACGI57_003583"
     CDS             194..2266
                     /gene="ptsG"
                     /locus_tag="ACGI57_003583"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HFG9718629.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGELLDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT          891 a          258 c          455 g          804 t
ORIGIN      
        1 ctgataatgc aggcaagact tatgtttata aaaaaattat aatagtttat ttttaattct
       61 gaaattaggt taatataggt ttgtttatat taacataagt ttaattttag aattaagtat
      121 actaatgtaa ctttttataa acttaagtct tttttattta aaaaaatata aaataataag
      181 gagagaaaag taaatgaaaa aagtatttgg tgttttacaa aaagtaggaa aatctctaat
      241 gctaccagtt gcattacttc ctgcagcagg tatattactt ggcgtgagta atgctttagc
      301 aggagacgca ttgttggctc attttccagc tttggcgaat cctacatttc aacttataat
      361 aagtataatg gaaagttcag gtcaaattat atttaataac ttaccactta tatttgcagt
      421 tggggtagct gttggtttaa ctgaaggaga aggtgttgca gcattggcag ccatagtagg
      481 ttttttaatt ctcaatacat ctatgggtgt tatggcaggt gttacagaag atatggtaga
      541 caattctatg tatgcaaata ttgttggaat accaacaatt caaacagggg tatttggtgg
      601 tattattata gggatagtgg ctgcttatct ttataaaaag ttttataaaa ttgaattacc
      661 tcagtatcta ggtttttttg caggtaaaag atttgtacca atcgttactt cattagttgc
      721 tatagcaata ggaattatat tgtcttttat ttggcctcca atacaagagg gattacttag
      781 tttctcaaaa aatgttatag aagcaaatca aacactagca gcatttatgg aaggttcttt
      841 ggaaagacta ttaataccat ttggtttgca tcatgttttc cataatccat tttggtatca
      901 atttggagaa tatgtaaata aagctggtga gcttgtaatg ggtgaccaaa agatattctt
      961 tgctcaactt aaagatggag taaatttcac agcaggtact tttatgacag gtgcatttcc
     1021 atttatgatg tttggtcttc cagcagcagc tcttgctatg gtgcatgaag caaagccaga
     1081 aaagaaaaaa tttgtagcag gtataatggc atcagcagca ttaacctctt ttttaacagg
     1141 aataaccgag ccaatagagt ttgcgttttt atttgttgca ccagtattat ttgcaataca
     1201 ttgtttattt tcaggtttat catacgcaat tatgcaatta ttaggagtta aaattggatt
     1261 cacattttca ggaggattaa tagatttctt attatttggt gttcttccta atagaactcc
     1321 atggtacttg gctatcttgg taggtattgg ttttgcagta ttatactata gtgtatttag
     1381 atttatgata agaaaattta atttaaagac accaggaaga gaagatgagt cagaaactaa
     1441 cgctgaagtc attgaaatgt ctaagaatga attagcatca gagattttga ttgctttagg
     1501 aaataaagac aacttaaaga gtctagatgc gtgtataaca agacttagat tggttgtaaa
     1561 tgatataaat aaagtagata aagaaaaatt gaaatcactt ggagcagcag gtgttatggt
     1621 tattggcaac aatgttcaag ctatatttgg accaaaatca gattcaataa agtctgatat
     1681 aaaagatata atagagggaa aaacaaaggt tgaaagtact gttactaaaa atgttgataa
     1741 aaataaagtg aaaaaaaata tggaagttgc aaatatggaa aatactttag tagaaaagat
     1801 tgattcatta acaaatggag agttattaga tatagaagaa gtgccagatt ctgtattttc
     1861 aagtaaactg atgggggatg gatttgcaat taaatctagt gatggattga tttattcacc
     1921 agttgatgga actattggag ttatatttcc aactaagcat gcaataataa taaaatccca
     1981 aaaaagtgga gtagaaatac ttatacattt agggatagaa actgtaaact tagaaggaaa
     2041 tggatttgaa gtatttgtga atgtaggaga tgaagtaaaa gcgggagaaa aattagttaa
     2101 aatggactta gaatatattg aaaaaaatgg tctatcaaca ataagtcctg tagtatttac
     2161 gaatttagaa caaaatcaga aattaagcat taaaaaagga gtaaccacag caaaagagga
     2221 gaatagagta agtatattaa gagatgatat tgaaaaaata ggatagctat atgatagtgt
     2281 aactaaaagt tatgtgagat ttaataaata tataaatagg ttcagttaac atgcaattta
     2341 tttaatatat attttaagta atagaaatat atatcattat attttgatat attttaaaac
     2401 attaaaaa
//
LOCUS       DAYAOX010000331         2354 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.336, whole genome shotgun sequence.
ACCESSION   DAYAOX010000331 DAYAOX010000000
VERSION     DAYAOX010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.336"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            127..1428
                     /locus_tag="ACGI57_003584"
     CDS             127..1428
                     /locus_tag="ACGI57_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFG9718630.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1504..1779
                     /locus_tag="ACGI57_003585"
     CDS             1504..1779
                     /locus_tag="ACGI57_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFG9718631.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
BASE COUNT          966 a          195 c          473 g          720 t
ORIGIN      
        1 tagtatatca tgacgaataa tacagtcata acaaatagtg agtcataaca aataatgtat
       61 ttataattga taatgtattt aaaaatcaaa atataaaaac ttataaggaa agagagatta
      121 atttatatgg gtaaatcaaa agatgttgaa atagagaagt ctattttagg cacaattctt
      181 ttagacaata aattaagcta taagctagat gaattaaatg aaaacatgtt tatgaatgat
      241 atatgtcttg agatatttaa aattatgaaa gaacttaaaa aagaaaacat agtgattgat
      301 gtggccactg tgaaatcaaa gatagataga aaatctctag caataaaaac aagtgatgtg
      361 actaatctaa ttacttgggg tcagaatttt ggtcttgatg gtcatattaa aattttaaaa
      421 gaaaatttag ctagaagaag cataaatcaa aactgccaaa accttttaca cagcctaaac
      481 cttggagaaa atatcgatac ttgtatttac aaatttgaaa gtaatataaa ggagattttg
      541 gacaaggata cttatgaaaa tgatgatgta aatagtatag cagggaaggt gcttgatttt
      601 ttagaaaaca aaaaagatat tggatttaaa tttggtataa aattacttga taccactata
      661 ggaggtcttt ttaaaggaga gctaaccaca atagcagcta aatcgggggt tgggaagact
      721 gctttggcac ttcaaataat gttaaattca tttaagcaag gtaaaaaaac tttatttata
      781 agtagagaaa tgaccagtga gcaagtattt atgagaaata tatgcagagt aactggtgta
      841 agtacaagga atatgaaatc taaagaaata gatgaaaatg attggaaact tattgtaaat
      901 gcgataggtg atttaagcga aaataactta atttatatca atgataagat agatacaatt
      961 agtgcaatta gaaagaggat aaggcaggta aagccagatt tattaatagt ggattatgtg
     1021 caactcctta catctcagaa aagcatggat aagagagaaa gggaggtagc tactttttcg
     1081 agagaactta aaaatatgac tttagatttt aatatacctg taatccaatt aagtcagtta
     1141 aatgatgaaa tgaaagattc aagaccttat ggagatagac ctatgaggga tagcaaagcc
     1201 atctaccacg atagcaacaa tgtggtttat atacatcaat taaaggggag tgattatgaa
     1261 gaagcagtga gagatattgg agaaagtgag gaggcagtta gagcatcaga atacagggga
     1321 attaagatgg ttgatttaat agttgcgaag tgtagagatg gacagactag gcataagcat
     1381 ttttgttact ttggggataa gttacatttt caagaactaa attattaggt caataaagta
     1441 gtaataacaa gcttattaaa agtcgggaaa tagcaaatag attataaaat atgaggtgaa
     1501 aatatgttgg aattaagaaa aattatagaa ttacttgaag aacacaagat agaagtaagt
     1561 attccaaagg agttacataa gtatagttta ttggaatacc ttgaattgtg tcaacaaagt
     1621 gggggaacat atttttatat taaaacactg gaaggggctt taaaagaggc tagaaatgag
     1681 cttatgatta aattgtttaa tgggaaaaat tatatggaat tagcgaggat gtttgaaatg
     1741 agtgttatta atgtcaggca tattgttaga aaaaaataga tatttttgga gaatacaagt
     1801 gtgatatact tctgaaatag aagttattat aagaggagta taagtgtgat taacaacatg
     1861 attttgtgaa taaaattgag gatatatatg agttaaatgt gtgattaata aggtaagatt
     1921 gtgtataaga ttatgagtat ttaaaaggag gggctataat taatagtgta gagttgtgtg
     1981 tttgcttaag attatataat aatttaatag attaaactat actaataagg taaaatcgtg
     2041 tgtttagtta aagcttatta taaaatcttg atatgtgatt aatgatgcaa tatgtgaata
     2101 aagttaagat tataatagat ttaacctgtg gctaatagaa taaaattgtg aataaagtta
     2161 agattataat aggtttaaat tgtgactaat agagtaagag tgtaaataga gataaacttc
     2221 aatatgagct taacctatga ttaatagagt aagaatgtaa atagagataa acttcaatat
     2281 gagtttaact tgtggctaat agagtaagag tgtaaataga gataaacttc aatatgggtt
     2341 taacttgtgg ttaa
//
LOCUS       DAYAOX010000332         2319 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.337, whole genome shotgun sequence.
ACCESSION   DAYAOX010000332 DAYAOX010000000
VERSION     DAYAOX010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.337"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..794
                     /locus_tag="ACGI57_003586"
     CDS             <1..794
                     /locus_tag="ACGI57_003586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HFG9718632.1"
                     /translation="GNIELSRGDTMKNKIEISMNNFVKNYHKSNNITTKWQELLFAYA
                     SAEDDMFQKLKSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWAR
                     AYIETNMLISNLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGT
                     FGLNNMLITEKGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALK
                     EDGFNRNKCYEMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1103..1381
                     /locus_tag="ACGI57_003587"
     CDS             1103..1381
                     /locus_tag="ACGI57_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HFG9718633.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            1674..2249
                     /locus_tag="ACGI57_003588"
     CDS             1674..2249
                     /locus_tag="ACGI57_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HFG9718634.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
BASE COUNT          915 a          224 c          411 g          769 t
ORIGIN      
        1 ttggaaatat agaattatct aggggtgata ctatgaaaaa taagattgaa atatcaatga
       61 acaactttgt aaaaaattat cataaatcaa ataatataac tacaaagtgg caagaacttt
      121 tgtttgctta tgcatctgct gaagatgata tgtttcagaa gctaaaaagt gttgtaggaa
      181 catctcatgc tatgccaaaa gattttctgg aaaatggaaa aactataatt acatatttta
      241 taccttttga tgaaagtgta aataatagta atattggagg gtttgaatgt tctgaaatat
      301 gggcaagagc gtacatagaa actaatatgt tgatttcaaa tttaaatgat ttcttatgtg
      361 aagaactaga gaagatgggg gctaattcat atgctattcc tgggtcgtat aattttgaca
      421 gtgaaaaatt ggtaagtgac tggtcacaaa gacatgtagc attcatagca ggtcttggaa
      481 cttttggttt aaataatatg cttattacag aaaaagggtg ttgtggaaga ataggaagca
      541 taatcacaag tttagaatta gagccaacta aaagaccaga aaatgaatac tgcttgtata
      601 aacataataa tagttgcaaa aaatgtgtca acaaatgcgt gaaagaagct ttaaaagaag
      661 atggatttaa taggaataag tgttatgaaa tgtgtatgat aaatgaaaaa aaatacactt
      721 atataggtgg atgtgatatt tgtggaaagt gtatagttgg agttccttgt tcagtaataa
      781 acccatcaag ataatggatt taagttgact aataatttta aagatagttt ataatgaaaa
      841 aattttagtt atcaaaataa atataatata aaaactaaat aaatgtgttt aaaaaatgga
      901 taaataattt gtgttgattg acaaccagtt atactgttga tagacttata tgtatcgctg
      961 tgatatataa ggagggttga gattttatgt ttgatttaat ctttattttt gcatagcaag
     1021 ggctattgca actatgaaat ttaatatata aatagccctt tgctattcct aatgaaaaat
     1081 tactatttaa taggagagca aaatgagcta taaaaaagca atacatatat taccagaaga
     1141 attacttgaa ttaatacagg aatatgtaga tggtgaatgt atctatattc ctagaaaatc
     1201 taataataaa aaagaatggg gaagtaatac acctactcgt gaggagttgt caattagaaa
     1261 tatgcagatt tacgaagact atcaagttgg atatgatttg caatatttat cggaaaagta
     1321 ttatttatct ttgaaaagta tccaaagaat tgtattacaa gaaaaaagaa aagatatcta
     1381 aggatgagcc agtatagttt tataattata ctggctcatt ttcttttgat aaaagtgttt
     1441 gaggtatagt ttcttttaag tggatgatat aatttaagta atttcaataa cttatacagt
     1501 agctaacaaa gctggttgta aagtatctaa taagaagtag tagcttttta tatgtttgaa
     1561 attaaaattt attaaaatta ggaggtcttg ttttgaaaaa aatacttgat tatattccac
     1621 cttttcataa ctttctatta atttagtaat cttatagaaa ggaaattgca tttatgaaaa
     1681 aaattacttg ggaggtggaa tatatctccc agttacctgt aattaggtat tgtaaaaaat
     1741 gtggaaaaaa gactgaatat atttgttcag gtttatttag ggttaatgct caacataaat
     1801 atttggatat atggttaata tacaagtgtt caaattgtga ttcaacatgg aactcaacta
     1861 tatattctcg tgttaatcca aaaagcttaa atccagaaat actagaacaa ttccatacaa
     1921 acgattataa tcttgttaag caatatgcta tgaatatgga acttttacat agaaatggag
     1981 cagaagttgg tattccagag tataaaatag taggacaaga aattaatatt aatgacctta
     2041 cacaagttca tattattagt aaatatccat gtcagttaaa ggtatcaaca ttattacgtg
     2101 agaaactagg tttatctagg aaggtatttg acgaaatgtt ggattgtggg attattagaa
     2161 gtaactcagg agttgattta aagaaaggta aattaaatta tgaaattatg ttagatatag
     2221 gcaaggagaa gcaaaagtct tcaaattgat tagaataact aacttaatat attttcagga
     2281 acatcattta aaaaaatagt ggctttaaaa aatttaaag
//
LOCUS       DAYAOX010000333         2307 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.338, whole genome shotgun sequence.
ACCESSION   DAYAOX010000333 DAYAOX010000000
VERSION     DAYAOX010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.338"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            180..914
                     /locus_tag="ACGI57_003589"
     CDS             180..914
                     /locus_tag="ACGI57_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9718635.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDGYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(1057..1548)
                     /locus_tag="ACGI57_003590"
     CDS             complement(1057..1548)
                     /locus_tag="ACGI57_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFG9718636.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1897..2280
                     /locus_tag="ACGI57_003591"
     CDS             1897..2280
                     /locus_tag="ACGI57_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HFG9718637.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYNG"
BASE COUNT          898 a          252 c          330 g          827 t
ORIGIN      
        1 acttttttgt ctattatata taaggaaaaa ttttttaata atataaatca tattcattta
       61 tattattaaa aaatttgttt atagaaactt ttattatttt atataaaagt gataaaaact
      121 tatattttgt atttaataga gtgttgctaa gtgaatatat ttaaaatgga ggaaacttta
      181 tgaaacaaaa taaatatgat gatgatagat tttttaataa atacagtaaa atggaacgtt
      241 ctacaaatgg attagcaggt gctggagagt ggcatgtttt aaaaaaaatg ttacctgatt
      301 tcaaaggaaa aagagtttta gatttaggat gtggatttgg atggcattgt caatatgcag
      361 ttgagaatgg agcaatttct gctgttggag ttgatatttc agaaaaaatg ttaaaagaag
      421 ctagaaacaa aacgcaactt gataatataa attacatatg tatgcctatt gaagatatta
      481 attttccaaa agatagtttt gatgtagtta ttagttcctt ggcatttcat tatattcaat
      541 catttgaaga tatatgtaag aatataagta attgtctttc aaataaaggt aattttgtat
      601 tttctgttga acatcctata tttactgcat atggaagtca agagtggtac tgtgataaag
      661 atggcactaa tctacactgg ccagtagatg gatattttga agaaggagta cgtaagtcaa
      721 attttctagg agaagaggtt ataaaatatc ataaaacatt aacaacttat ttaaatacgt
      781 taatgaaaac aggatttgaa atattagaaa ttattgaacc tcaaccagaa gaaaaactgt
      841 tagatacaat tcctgatatg aaagatgagc tacgtagacc tatgatgtta cttgtatctg
      901 caaaaaaaag ataagtaaat ataaataatc attattgtag aaaaaaagtt gtagaaaata
      961 attacactaa tggagatgat tataccagtg gtattaatta caccagtagt aaggaggcaa
     1021 aagcattcgt tgcttttgcc attttttaag ttgcaattat acttctacgt agtatatttc
     1081 gtttccattt tcttctataa gctttttcat atcttcaaat tttaaaaata cagtagcagt
     1141 attatcattt ggatgtatac ctattttatc catttttaat aaatctttat caaatacaac
     1201 ttttacaaca gcctctttat catttaatat accaaaagga gtcacagctc ctttagtaag
     1261 tttcaagtat ttttgaagtc tttcttccga tgcaaaacta agaggtttac tatcaatttg
     1321 ttgacgaata tcttttaaat ctgctttttt gtctttacct aatacaacta aaaaatgttc
     1381 ttttccttta gcatcacgaa gaaataggtt ttttacaaca aattccatat gtaatgtttt
     1441 taattcttcc atttcttcaa ttgtataaac tggtggatga tttacagcct cataaggtat
     1501 gcttaactca tctaattttt tatatacttc ctgttgttga ttgttcatga tttcctccca
     1561 ttagaaattt attttatata taaataatat tatcacagaa aactttaaaa gaaaacgttt
     1621 tatataatat agacaataga ttatattttt ataaagaagc aagaatactc aataaaactt
     1681 aaagaaatta aacaatttct aaaaatacct tgaaaataga accttatttg ccatttataa
     1741 attatataaa aagggattat ataaagtcat atatgaaaaa actatattat actatctttt
     1801 ctttttggaa taaactatac tttaaatata gatatttaat ttaaataaat tttacagtat
     1861 taatagtaaa ttatcttaga aaggaatgtg ctttatatgt ctacagacaa agttgttgga
     1921 atcatagatg aagaaacggc agaacttgca ggaattgaat atactggtaa aatttatgct
     1981 tcttctggtg ttataaaaca tataaagaaa aaacatagat gtcaactgag taaggatatt
     2041 tttaatgata taatagacac tataaaaatg gtattaaagt cacctgaata tataggaagt
     2101 catcctaaaa aacctggaaa aagtgtggag tttataaaaa aaattgatga ttatatttta
     2161 gtagctacag aattagatac aaaaaaagga tatttatatg tatcttcaat gtatgctata
     2221 aaagaagcaa aattagaaag tagagtaaac agtggaagag taaaacaata taatggttaa
     2281 cataatatta ttaattttcc ctaaaat
//
LOCUS       DAYAOX010000334         2283 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.339, whole genome shotgun sequence.
ACCESSION   DAYAOX010000334 DAYAOX010000000
VERSION     DAYAOX010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.339"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            93..281
                     /gene="rpmB"
                     /locus_tag="ACGI57_003592"
     CDS             93..281
                     /gene="rpmB"
                     /locus_tag="ACGI57_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFG9718638.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(441..593)
                     /locus_tag="ACGI57_003593"
     CDS             complement(441..593)
                     /locus_tag="ACGI57_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HFG9718639.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(691..1167)
                     /locus_tag="ACGI57_003594"
     CDS             complement(691..1167)
                     /locus_tag="ACGI57_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFG9718640.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1386..2147
                     /locus_tag="ACGI57_003595"
     CDS             1386..2147
                     /locus_tag="ACGI57_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9718641.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
BASE COUNT          838 a          316 c          306 g          823 t
ORIGIN      
        1 tatatatttc tattgcaaat ttttgttatt tatgataata taaatatgtt tttagtcttt
       61 aaatagttat cattttaagg aggtgtgcgt agatggcaaa agtatgtagc gtatgtggta
      121 aaggtaaggt ttctggaaac caagtatcac actcaaataa acataataaa agaacatggt
      181 cagctaactt aagaagcgta agagcgatta tagatggagc tcctaagaga gtaaaagttt
      241 gtactagatg tttacgttct ggtaaaattg aaagagctta gtaaccacgt ttaaaagcct
      301 acttccgtaa gtaggctttt taatttaatc atttttactc tttctcataa attttaggat
      361 tatacttgaa atccattttg gtaattttat tgttactact ttcatcaata ctcaccccac
      421 actttatagt taaaactatt ttaacaaaaa aataataatg ctccacatcc aataagtatt
      481 aatgcggtaa gtgctgacca aatccagcca ggaagcaaca ttgacagaat tacagccatt
      541 cctgttgcta gacaaagtat tcctagcaat accttagact tgttatttct catggaacac
      601 ctccacatag aacctgtatc tataaatatt ttcatattca agtttatgcg acagttttcc
      661 attttagacc aataaacaca ttatgtaatt ttaatttttc atactagaat tattactgaa
      721 ctccgatata gattctacaa tatccaaaat tccttttttt ctacttaacc tttcaggtcc
      781 actatataaa acaactggta taactttagg aagtttaaaa tttttatctt ctatttcttc
      841 aaggcttttt ttcataataa aacttttcca aatttgttct ctatacgatt tcaatcttgg
      901 gacaatttca gtatccattt ctgtttgaaa ttctaacact atataatgtg atactttgcc
      961 gcttttagaa aataccttat aaattatatc aacttctttt cctttatatt cacatataaa
     1021 agatgtttct aactctatat ttttaagagt taattctttt gctattgaga cacttgtaaa
     1081 attttgaatc aaacttaaaa aactttcttt atctgaatac attcttctgt actcttctcg
     1141 tttttcattg cttcttttta aattcataca gttcctccta gactatagac cttctatttt
     1201 ttagtaaaga tagtctataa attttatttt ttaattttca ctagtgagta ttgacagttg
     1261 tgtgtaaaat atccagtaaa gatatttttt tatattttta ttaaaagata taaattttta
     1321 tattttaatg tattgacatt tacgttgcgt aaaagtgtat atttaattta taggaggtac
     1381 tacttatgga atatacagtt caaaaattaa gtaaactagc tggtataagt actagaacac
     1441 ttagatatta tgatgaaata ggtcttctta agccacttaa aataaattct tcaggttatc
     1501 gtatatatgg tcaaaatgaa gttaataaac ttcaacaaat acttttttat agagaattag
     1561 gaattaactt agaaaatata aaaaacatta taaattcacc aaacttcgat agtttaagtg
     1621 cgttaaaaga acaccataat aaattactag ccaaaagaaa gcaaattgat ttactaatag
     1681 aaaatgtaac taaaactata gctctgaagg agggaaaata cactatgaca gatatagaaa
     1741 aatttgaagg ttttaaagaa aagatgattg atgaaaatga aaaaaattat ggaactgaaa
     1801 ttagagagaa atatggtaaa gatattatag atacatcaaa taaaaaactt aaaaatatgt
     1861 ctaagcaaga ttatgaaaat tggcaaaacc taaatatcga aataattagt aaacttaaaa
     1921 aagcttttaa aactggtgat gcctctagtg aactggctca ggaagttgca aaattacatc
     1981 ataaatggtt gtgttacact tggaacacat actctaaaga agctcattcc tctctagcac
     2041 agatgtatgt atatgatgaa cgttttacat catattatga tagagaacaa cctggattag
     2101 ctaagttttt aagggatgct atagtaatat atacaagaga taaataatat aaaataataa
     2161 aggtggtgtc tcaaaatgaa ctttttagtt catgagacac tcttttttgt atgaaatcag
     2221 atatattttt atcattttat caatgaaaaa caggtttctc tctctatttt gagacagaat
     2281 ctt
//
LOCUS       DAYAOX010000335         2279 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.340, whole genome shotgun sequence.
ACCESSION   DAYAOX010000335 DAYAOX010000000
VERSION     DAYAOX010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.340"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(154..1721)
                     /locus_tag="ACGI57_003596"
                     /pseudo
     CDS             complement(154..1721)
                     /locus_tag="ACGI57_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(1714..2239)
                     /locus_tag="ACGI57_003597"
                     /pseudo
     CDS             complement(1714..2239)
                     /locus_tag="ACGI57_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ia"
BASE COUNT          714 a          312 c          256 g          997 t
ORIGIN      
        1 gataattatc tttttaatac aatatagttc taaaaaagaa tatattagaa gaagataata
       61 tttaaataaa aaatacctta taaagttaat tttttaatac tttataaggt aaatacatat
      121 tgtattgtgg ggataaattt aaatcatttg atactaatca acactaagaa ccaataactc
      181 tctatcatct tgagtaattg tatatattct taatttcttg tatgtcataa tattttctcc
      241 acctgtaaaa taactcctaa gattaacaca atcagtttta ggttgatttg tatctcctaa
      301 ataattacgc aattctttat ctttagtttc aatatcttta aatccaaaat attgtaaagt
      361 agcttcaact ctatattttt ggacataatc gagagcttct ttattagttt atgttggtgt
      421 aaaatacata ccatttatat aagcctttat tgtacttgga ttaaatttaa aatctgcaga
      481 atatatttta tgtgtaacta ttatttcttg aacagttgga attttaactt ctggttcatc
      541 aagtatttca ggagtactat ttagctctgt aatagataag ttatctaagt atattgtatc
      601 actatcaatt aatgctatat ttatattaga agaatctttt tcagtagttt caaattcata
      661 actaaatttt gtatatcctt gttctagtac caaataatcc gttttttctt cttttacttt
      721 tatatttaca attattgaat tatatgttaa aggatttttt gaattttact aaaaacataa
      781 cgtttataag gttttagttc agacataggt attttaatct tcgtctcacc atttaatcta
      841 tttgctgagc cttttaaacc atcttgattt gtagtattaa cattactcca tatactaggg
      901 taattattgt aatcatcaaa attagtaaag taagttggtg tttttataag tatattcatt
      961 cctctttcaa gtttaacatt acatatttgt ttatcagaca aagtttttaa actgtcttta
     1021 atcttattgc ctatattatc atcaaatatg agttcaacac aactttcatc tattggtata
     1081 ttattaaaat ataacaaacc atctttttta gtagtgccaa aagttttttc aattgcttct
     1141 ccaattgtaa gttcaggtgt tttatcttct gggacctgta aacttttagc agtaattctt
     1201 cttttgtaag attcattttc tgtatctaat ataatagata cagaaatact gtcaatttga
     1261 cctatatcgt cttaccaact atttcattct gttactattt gtccagaacc attttttgta
     1321 ccaaaatttc cacttacttg tgttgtttct aatttaattt gctttccatc atctaatttt
     1381 ttaaattaat tataatttat aggaatcagt ctagaactaa attgatacat tgtgttaaga
     1441 gccataggtg aaaatccttt tttgagataa gtatttccag gagatagatt attgccaatt
     1501 tgattttctt gtgctttgat agttgataat gcatctccat ctaacactaa atttgttgtt
     1561 ggtgtcactt tgtacatagg tgcagtacct gtgttgtagt atctaatatt tgcatttata
     1621 tatgcagatt ctcctttgtt tatacttaat ccagtatttc atgattatcc attgctattt
     1681 tgaacagcag ttgaattatc tgttgtatgg taataatttg tcttgtatag tttctttaaa
     1741 ctcattaaat ttttctaatg aaatttcgtt ttgatttttt gttcttatta ctttattaaa
     1801 tgcaaatttt tctggagatt caaaataata tacatacata ggtttatcta ttttattttt
     1861 tgatatagca tttcgaagtt ctctatattc tttttctcaa gaatttgctt ctatttgata
     1921 atcataaaaa taatttcttg tctgagaata tttgcttatc tctatagaat cttttttata
     1981 tgatcctaat acttcttttt cagatttttc aagtttttga tcttttcttt ctcattcttt
     2041 agccttttct ttatctttaa aaaaatcctt ctgatctttc tataggagcc ttgtaagtag
     2101 tattacaaac ttttccacta tagctttgta aatcttgtgc ataaatgtta ttaggtaaca
     2161 aactacctaa agttagatat aatatcaaca atatagatat acaattacta atacttttat
     2221 gtttcctaaa ttttttcatt tattctccct acaaatatta gtttagttaa cttttgaat
//
LOCUS       DAYAOX010000336         2260 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.341, whole genome shotgun sequence.
ACCESSION   DAYAOX010000336 DAYAOX010000000
VERSION     DAYAOX010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.341"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            504..2033
                     /locus_tag="ACGI57_003598"
     CDS             504..2033
                     /locus_tag="ACGI57_003598"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase domain-containing protein"
                     /protein_id="HFG9718642.1"
                     /translation="MKSLKALIVGVSKYSIVKNKDLLFCKNDIIEINNVLHKRLGITS
                     NNIRTLGMEYEVRKDLFFKTFQEISDSIGDDDTFIFYFSGHGCICNNGVHYLVLSDEL
                     VSIQFVIDFIEGLNCKNKIVFIDCCFAGKFNVSDKQKINIKDIFSLFDRKGCAVLVSS
                     SEYQESFSLPDSSISRFTSLLSEALRNEYIVRKGELSLRAISKYINLRMSILNKNYIN
                     CKQNPIFRSNILGDIYFNVKDYKSYIKKKVFQEHDDYIIYNVKSLHISIIKRYSVEVI
                     LKNKFSYCDISEISKEVFDIVKSVEVYNSEIGEDRLKGKDANIIWVYFGKDEIDMING
                     IYMCKTTWIDKYQEKDIWYSVNGKNKFFINGVHTETYDNYESLKSYISQNTITKEKAV
                     YEVKEILTNMINISEEIISFYNEYENKKICEDNIFQIIDSRKKKMDECYNKVINLDFP
                     PIEIKDWFDKCINLVSTIQNFGVYWDHSRTEENRKQCMEMNIEDYYKDLREVIRLSEE
                     I"
BASE COUNT          978 a          170 c          355 g          757 t
ORIGIN      
        1 ttttagaaaa aaagtagcac aaattaaagg tttttaaaga tgtatgtcta actttagaaa
       61 tgtatgtata attttataaa taaagcataa caatttaaat aaatacttga gggaataaaa
      121 tgatattaaa taagatataa atgattggaa atatttactc actttaaaat tcttatggat
      181 agtagaatac aaaaaacaca tgaaattcta ttaacatcaa tattttgggg aattttaaaa
      241 aatatatggt gaagagatag atgattttac attggtttaa attacactaa gttcaggtca
      301 gataaatcag agatataaat tatatgggtg cgatgtaaat aaatatatat atacttacct
      361 tgaaaaatta taatccaact acaaaagtaa ataatgaaaa agtcaaaaaa gtagtgtgat
      421 actagctttt ataaaatcaa taaaacaatt aaaacatcat tttaagtata ttaaattgaa
      481 aaattataaa gggggaatgg aatatgaaaa gcttaaaagc attaattgta ggagtaagta
      541 aatatagtat agttaaaaat aaagatttac tattttgtaa aaatgatata attgaaatta
      601 ataatgtgtt acataaaaga ctggggatta cttcgaataa tataagaact ttaggtatgg
      661 agtatgaagt cagaaaagat ttatttttta aaacttttca agaaataagc gattctatag
      721 gagatgatga tacatttata ttttactttt cagggcatgg atgcatatgt aataacggag
      781 tgcattactt agtattaagt gatgaattag tttctattca atttgtaatt gattttatag
      841 aaggattaaa ttgtaaaaat aaaattgtat ttattgattg ttgctttgca ggtaaattta
      901 atgtaagtga caaacaaaaa ataaatatta aagacatatt ttccttgttt gatagaaaag
      961 gatgtgcagt gttagtatca agtagcgaat atcaagaatc attttctctt ccagatagtt
     1021 caattagtag atttacaagt ttattatcag aagcgttaag aaatgaatac atagtaagaa
     1081 aaggggagtt atctttaaga gcaataagca agtatattaa tttgcgtatg agtatattga
     1141 acaaaaatta tataaactgt aaacaaaatc ctatctttag gtctaacata ctgggagata
     1201 tatattttaa tgtaaaagat tataaatctt atataaaaaa gaaagttttt caagagcatg
     1261 acgattacat aatatacaat gtcaaatctt tacatataag tataataaaa agatattcag
     1321 tagaagttat attaaaaaat aaattctcct attgtgatat atcagaaata tctaaggagg
     1381 tttttgacat agttaagtct gttgaagttt ataatagtga aataggagaa gatagattaa
     1441 aaggaaaaga tgctaatata atatgggtat attttggcaa agatgaaatt gatatgataa
     1501 atggaatata tatgtgtaaa actacttgga ttgataaata tcaagagaaa gatatatggt
     1561 atagtgtaaa tgggaaaaat aaatttttta tcaacggtgt tcacactgaa acatatgata
     1621 actacgaaag cttaaaatca tatataagtc aaaatacaat cactaaagaa aaggcagtat
     1681 acgaagttaa agaaatttta acaaatatga taaacatatc tgaagaaatt atatccttct
     1741 ataatgaata tgaaaataag aaaatatgtg aagataatat ttttcaaatt atagatagta
     1801 gaaagaaaaa aatggatgag tgttacaata aagttataaa tttagatttt ccaccaattg
     1861 aaattaaaga ttggtttgat aaatgtataa acttagtgtc aactatacaa aattttggag
     1921 tttattggga tcatagtaga actgaggaaa atagaaagca atgtatggaa atgaatatag
     1981 aggattatta caaagattta agagaagtca taagattaag tgaggagata tagaattaaa
     2041 tttttccata gttgtactaa tatagaatta taagtcagat tggtactttg ttggttacga
     2101 atttaaatta aaataagttg tttacactaa acatgataga agatacaatt ataaatccat
     2161 ttataaatta tattggtatt gatttaaata attagatatt tattttgaaa aagtctagta
     2221 aatatttaaa aacgtataat atgctgttaa tggaactttt
//
LOCUS       DAYAOX010000337         2230 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.342, whole genome shotgun sequence.
ACCESSION   DAYAOX010000337 DAYAOX010000000
VERSION     DAYAOX010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.342"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            112..1017
                     /locus_tag="ACGI57_003599"
     CDS             112..1017
                     /locus_tag="ACGI57_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HFG9718643.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            1026..2210
                     /locus_tag="ACGI57_003600"
     CDS             1026..2210
                     /locus_tag="ACGI57_003600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9718644.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
BASE COUNT          920 a          183 c          386 g          741 t
ORIGIN      
        1 tttatatttt aaacaatata aatgaaatta ataccataat aaaattggag ttttttatat
       61 atacaaaata aaatttttaa aattaagcaa taaaaaaggt aggaggattt tatgtttttc
      121 aaagatattg aactaaattc aaaaaaagag ttagacccat attttgattt agtagattat
      181 gaagcttgtg agtactgttt tagtacttta tatatgtggc aacatgttta taagacagga
      241 tattatatag gtgaagattt tgccgtttta gttggggaat acgaaggaga tagcttctca
      301 atattaccac ttgctaaaaa agataaactt ccagaagttg tagattttgt ccttgaatat
      361 ttttctaaaa ataataaaaa aatatactta agaggtatta ctactgaagt agtagagttt
      421 ttaaaagaaa aatatccagg tagatttgag tatatagaag aaagagattt atttgactat
      481 atttatgatg cagaaagttt aagaactcta gctggtaaaa agaatcaaaa gaagagaaac
      541 catataaact atttcttaaa agagtatgca ggtagatatg aagctaaatt attagataaa
      601 gaaaactttg atgagtgttt agtactaatg aaggaatggg aaagtaataa ggaagaaaat
      661 aatgaatttg atgaaagtat ggatgatgaa ttaataggta tcaaaaagat atttaaccat
      721 tatgacatat taaaagataa ggtaaaagtg tttggtgttt atgtagatgg aaaattagaa
      781 gcttttagta taggagagct tttaaatcca aatatggcat tgattcatat agaaaaagct
      841 aatcctgata taagaggtct gtatccattt ataaatcaac aatttttggt aagtgaattt
      901 aaggacgttg agtttgtaaa tagagaagaa gatttaggta ttgaaggatt aagaaaagct
      961 aaactttctt atcatccttg cagatttgtt gaaaagtaca gtgttaggga ggcttaatag
     1021 actatatgga gattaggtat gctaaagaag aagagataga aagtataaaa gagatttgga
     1081 gttattgttt taacgatacg gagagtttta tgaagtacta ttttaatgat aaatataaat
     1141 ctgaaaatac tgtagtagct ttagatgaag gaaaaattat ttcttctctt cagctaaatc
     1201 agtataagtt attactaaat agcaaggtat ataatacatc atatgtagta ggagtttcta
     1261 cacttccaga gggaagagga gctggatata tgaacaaagt aatgaaattt actttaaacg
     1321 aattgtataa aaaagggcaa ttagtttcta tattgatgcc tattgactat agactttata
     1381 gaagatttgg atatgagcat tgttacgacc agattgaata tactattaac acagatgatt
     1441 taaaaaattt taagtcaagt ggaaaaatga taaagtctaa tttatcacaa attgatgatt
     1501 tgatacaaat agatagaact tttttaaatg aggttaatgg taatgtgtta aaagatgaac
     1561 attattatga aaacttattt aaagagattc aaagtgaaga tggattttta tacattcatg
     1621 aaggaaatga aaaagatgga tatatagttt attttcttca ggaagataaa atgtttgtca
     1681 gagagttatt ttataaaaat atagatgccc ttaaatcaat gcttaaattt atatataatc
     1741 acaatactca atgtaagata gttactatat ctactcctac aatagataag ataagattta
     1801 tattagacaa ccctaaagat agtgatataa aaataaaacc ttttatgatg ggaagagtta
     1861 taaatgtaaa gaagtttatt gaagatatag atattgagaa tgatattaat agctcattta
     1921 atttattaat agaagacaag tttatagatg aaaataatgg tctatttaaa atttctatac
     1981 agaataagaa ggtatctgta gagcagttag ataaaaaagg tgcagaaaag cctcaagaag
     2041 attttgatat taaattagat ataaatacct taacacagtt atcattttct tatatagatg
     2101 taaatgaagc tattttctta aatgatataa aagatgtttc agaagaaaca ctagaaacac
     2161 ttaattgtat tttttctaaa aaaaataact atataaatga atatatatag gtagagtacg
     2221 ttaaaagatt
//
LOCUS       DAYAOX010000338         2213 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.343, whole genome shotgun sequence.
ACCESSION   DAYAOX010000338 DAYAOX010000000
VERSION     DAYAOX010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.343"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(66..980)
                     /locus_tag="ACGI57_003601"
     CDS             complement(66..980)
                     /locus_tag="ACGI57_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HFG9718645.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            complement(1807..1989)
                     /locus_tag="ACGI57_003602"
     CDS             complement(1807..1989)
                     /locus_tag="ACGI57_003602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718646.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT          801 a          280 c          282 g          850 t
ORIGIN      
        1 ggtacagtta actgtacctt tattttatct aaatatgcaa agattatttt aaaagtaatt
       61 tttaattaca tgtttttata actctccaat attctaatat aatgatttgc ctctcttaaa
      121 acatggtctg ctaaaagtgg taatattata gattttatct tacactgttc tattcctgat
      181 gctcctgctt ctttaaaatc tttaaactca acagtttcat ttagagtttc ttctgtaatg
      241 ttcttgatat tagaatcagt catttcgttt gttttttcaa ttaattcatt aaattttata
      301 gcaaaatcat ttgaagtatt tattagttca ccttctgagg ggtctagtaa tcctctcata
      361 aacagagcat gttccatcat aatatggtcc cagaataatt ctatttcttt agcgtttttt
      421 gactcaatat ctattttatt ttcaaggtca actacataag aacgatataa atttgcttca
      481 tgtattatat gttcaagaag tagagggtag tttgatgtaa atatagtaca tgatagtaca
      541 ccatctaaga ctctttcttt aaaattaata agcccatcaa gtaatcttat tgcatcagag
      601 ttaagttgag ctacatagtt cactaagtct tgaccaactt gtgggtttac accactttgt
      661 agatttaatt ctcttgtagt gatgtttttg tttatttcta tccctgtaaa ctcttctgtt
      721 ttttgttctg caactgatgt gagagtagtt acaagctctt ctgaatataa tatatcaggt
      781 ctaattatac cattactagc actaacagtg tatgataata aatcttcaaa ttgcttttta
      841 tagtggtcag cttccatagc aagattataa tttttatttg tgaatcctgc ttctaaaaaa
      901 agagcatgct ctttcataat tcttgaaaaa aataaatgta attctagtga taaaataaca
      961 tatttttgat tatctatcat aatttcctcc ccttagaaag tatcctattt ataatacttt
     1021 attcgtaata tttaaattta tgtatattat aaataaatta actaaattca attgctacta
     1081 atatcaacag tttttattaa gttatgggcg aaaagattat cttactgtgt ttcttattag
     1141 atatagatat aatgatttaa ggtacttgtt ggctattatt tattaagagt acaagtaata
     1201 ctagaaattt ttatgagaat tatgtaattt ataagtatta ttttgataca aaacaaatgt
     1261 tttttgtaaa acaagaactt ttatagcaaa aaatgaataa atatgaagca agcataattt
     1321 tataccacaa caattattaa ttctaatagc tgcacttaat gttttaggta tgggatttaa
     1381 aaagtataaa aaattaagtg ataaatatat tccattatat tgcttgtgct tggagtagta
     1441 ttttctgtat agatgttagg atttaatcca acttctattt tacaggggat tctatgctgg
     1501 ggtgtttcaa ttggattaaa ttaagtatgc aaacaatttc aagacaatga taaataattg
     1561 gatgtgtgat aggaatatca acaaaaaaca ttattaaaaa gaattatttt ataaataaaa
     1621 caagtaataa gaataaataa ggagcaacta aaatgattta aatatactag aattaaaaag
     1681 agaatgtaaa gttgtctagt aatggatgta aaatatttta aacgataagc tatgaaattg
     1741 atatattaaa atgggtaaga agaatattat ataggattct tcttacccat tttaataaat
     1801 ttaattttat gccaaaccac attcgcatag tagaatcatc actctatgga gacataactc
     1861 tacaacatct tctccatatt ttttaataaa atcaccaatt tgagtttcta tgaatgaata
     1921 gtattctttt aaactagcaa tatatgcagt cattatatga acacattcat caatcttatc
     1981 tttaaacaca actaatcccc ctaaaaaacg aaaaataaaa atattcagta aatagcagat
     2041 attatttcac tcataaatct atatagtatg atatattgct tagatatatt ttacaagtgg
     2101 atatgtaaaa ctggttatta taatcgcaaa actggataaa ctttcattct taaaagaaaa
     2161 tttatccagt ttaaagttta attatgaata tttgagttag tagatattta ata
//
LOCUS       DAYAOX010000339         2148 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.344, whole genome shotgun sequence.
ACCESSION   DAYAOX010000339 DAYAOX010000000
VERSION     DAYAOX010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.344"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            142..981
                     /locus_tag="ACGI57_003603"
     CDS             142..981
                     /locus_tag="ACGI57_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9718647.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITKYLYFNGL
                     DGK"
     gene            1096..1335
                     /locus_tag="ACGI57_003604"
     CDS             1096..1335
                     /locus_tag="ACGI57_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718648.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1506..1682)
                     /locus_tag="ACGI57_003605"
     CDS             complement(1506..1682)
                     /locus_tag="ACGI57_003605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718649.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1736..1832)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          883 a          200 c          325 g          740 t
ORIGIN      
        1 ttttaataat taattattag gatgtttatt ttcctatcct tgttttaaag atttttttag
       61 tatattctaa tactatagta ttatagagta ataatgttga tttaaaataa aaataagatt
      121 tcggaggagt ttattatata aatgaaagat tactataaaa taggagagat atctaaaata
      181 tatggaatag gtagagattc attaatgtat tatgaagaaa taggcattct aagaccagtt
      241 agagatataa atggatatag aatgtataat atatcagata tatggaaatt aaatttaata
      301 aaagaattta gaagcttgaa ctttccaatg aagaaaataa aagaatacct agatgacaga
      361 agtatagagt ctacgaaaaa tattttaaat gaagaactcg atttaattga taaaaaaata
      421 gctgaattta taagccataa ggaaaatatt ataaaaagat tgagttcaat tgaaagtgta
      481 attcaaaata caaaaataga tgagatagag gttgtatata tagaaaaaag aaaagcttta
      541 gaattaaatg ctgatataaa gagggatgaa gacttcgatt ttttaattca aaaacttcag
      601 aaagaatatg aagatagatt taatatactt ggaaacaaca atattggttc tgccttttct
      661 actgaagcaa ttaataaagg catatttaat gagtttaaat ctgtattttg ttttctggaa
      721 agtaatgaga aagtatataa tatagttttt gatgaaggat attatgtaac tttaaattat
      781 actgggagta attctaataa taagatgtat atggagaagg tgtttaaatt tatagaagaa
      841 aataattata agataattgc tgaccctata gaaatctata agatagatat acatgagact
      901 ggaattgtag aggagtttgt aacagaaatt caagttccta taactaaata tctatatttc
      961 aatggattag atggtaaata gaatgaaaaa atgttgattt ggcaccgatt tgccactgtg
     1021 ttataaaatc ggtagcaaat tatattttaa attataagat agcatgaaaa aataattgtt
     1081 tagggggtta taaatgtgaa atgttctaat tgtggaagtg taaatattga aaagggaatt
     1141 ttaacaactg gtggcatata tgctgaaact attggattaa aatatagagg agatggaaaa
     1201 ttagataaag ttataaataa aacttttcca gtacaatcta ctatatattc agatttatgt
     1261 ttagattgtg gcgaaatagt tagaacctat ataaaggata atactgatag gaattggtgt
     1321 aaagataaga aatagaataa tttttgaagt tgaattttat taaagataca agtgaaaaag
     1381 tataaatggg aagaaattgt tatatggaag aaaactttgc atatatggaa gattagtttt
     1441 ttaatataat aacattttta aatgtagata aaactaaaaa gggttctcta gaaaacaatt
     1501 tctatttaat tatacttact taccaatacc atcacttgat gtaaacaaga ttctactaca
     1561 ttttctccgt actctagtat aaaatcatct agctgtgtat ctataaaatc acaataatca
     1621 tataaactac taatataagc agttaacatg tgtgtacatt tatccatttc atctctaaac
     1681 atatatatta ctatccccct aaaaaacttt cataaaacaa aaagaacaat atctttttgg
     1741 caactggctg acataactca tagtttattt tataagcgtt agtccctata gctttgcgtc
     1801 actaaatttc tctagttttg ccgatttagt tttattctac aactaaaata atacactagg
     1861 ataagttatt gttcaacata cttgtcagaa aattgaaata tttaacaata tcttaacaat
     1921 atgaaatcta tttttcactc aagaaacaaa ctatatgtat agaatgttta ataaaaatta
     1981 tatttagttt tgtattgaag tttttgtatt aaaaagttta gttcacaagg gttacttggc
     2041 tagataatag ataaacaaaa atatcttgaa aaaatatata tgaatggcaa aaaacaaggg
     2101 atgaatttta tatagactca tcccttaatt atttatatat tgaccttg
//
LOCUS       DAYAOX010000340         2111 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.345, whole genome shotgun sequence.
ACCESSION   DAYAOX010000340 DAYAOX010000000
VERSION     DAYAOX010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.345"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            497..1825
                     /locus_tag="ACGI57_003606"
     CDS             497..1825
                     /locus_tag="ACGI57_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9718650.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          758 a          229 c          355 g          769 t
ORIGIN      
        1 tatttcattt ttaaataaaa aaataaaata ttagttttga tttctggttt aaattgggtt
       61 ataataaatt gtcaaattat ttattaatat aaaatagact ataaaacaag caaaaatcat
      121 actattatat aaaagacctt aaagatagaa aactacagaa agaagccaaa atgtttttga
      181 aaaaaatgaa attttattgc aaaaaaatca aaatgaattt aacttaatag ttataatcta
      241 tttttagtgt tacaaatata caatgtatat tatacaatat aaaaaataca tttacttaga
      301 agtattatta tatttggatt tttggttttt ataatttttt tatatttaaa cataactaaa
      361 aataaaagat tactaaagct aagtagagtt tattttattt taataaataa taaagctatt
      421 tttctatata ttggcataat atatgcttat ttaactatga aattaaaatt aaataggatt
      481 aatgaggagg aaaaaaatgg gaagtaatga actgaaaaag actcttggcg tatcagcagc
      541 attatcaact gttgttggtt ccgttatagg agcgggcgta ttttttaaac cacaagcagt
      601 ctatacatta actggaggag ctccaggtct tggtatattg gcatggttaa tagctggaat
      661 cataactata acagctggtc ttacagctgc agaggtatca gttgcgatac caaaaactgg
      721 tggaatgatg gtatatataa aagaaattta tggagaaaaa ttaggttttt taactggatg
      781 gatgcaaatt gtattatttt atccagggat gatggcagca ttaggtgtta tatttggaga
      841 acaagcatcg gcactaattg gtagtccatc acttttactt ccaatagcaa ttggaattat
      901 aataatagtt gcagggctta atatgttagg ttcaaaaact ggaggagtca tacaaactgt
      961 ttctacaatt tgtaagttaa taccacttat tttaataatg gttgttggat ttattaaagg
     1021 tggtggaaat aatccaatac ttacacctat ggttggtgaa ggattaagcc tgggtagtgt
     1081 actaggtcaa gttttaattg ctatattatt tgcttttgat ggatggatga atgttggtac
     1141 tttagctgga gaaatgaaga atcctggaaa agaccttcca aaagctataa taggtggatt
     1201 gtcagttgtt atggctgttt attttataat aaatttagcg tatctttggg tattaccagc
     1261 taatgaatta gcaaattatg cttctcctgc atcagcagtt gctgaagtta tatttggttc
     1321 aatgggtgga aaaataatat ctgtaggtat attaatttca gtgtttggag cattaaatgg
     1381 atttttacta actggttcaa gggtggcata tactttagct acagataaga ctttaccaaa
     1441 gtatggtata ttttctaaat taaattctgc tcaagttcca gcaaatgcaa tagcattagt
     1501 atctgttatt gcatctatat atgctttgtc aggtcaattt aatttattaa ctgacttagc
     1561 tgtatttgct acttggatat tctatgtatt aacttttata ggggttatga aacttagaaa
     1621 gactcatcca aatataccaa gagagtataa agttccatta tatcctattg ttcctataat
     1681 agctatagca agtggtatat ttgttgtagt taatcaattg tgttttgcag gtatgaaaac
     1741 tactatgata tctataggag gtcttgtaat aacagctata ggcttaccag tgtatgctta
     1801 tatgactaga ggagtaaaaa gataaaccaa gtagtttaca aatgcttcat aattaaaata
     1861 aaagagactg tctcaaatag agataagtct cttttttatt gttaaaatgg taaagatatg
     1921 tttaatttta caaaaaaaga gcgttccatg aactaaaaag tttattttaa ggtattcatt
     1981 ttatttttag ttttttgatt ataaaattaa acatctaaaa aattatttat tttaaaatac
     2041 tttattttaa agtgtttttt atcaaactaa gtggtatctg tacaacaaat attatacata
     2101 gcttttatga a
//
LOCUS       DAYAOX010000341         2110 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.346, whole genome shotgun sequence.
ACCESSION   DAYAOX010000341 DAYAOX010000000
VERSION     DAYAOX010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.346"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(39..1256)
                     /locus_tag="ACGI57_003607"
     CDS             complement(39..1256)
                     /locus_tag="ACGI57_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HFG9718651.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(1447..1860)
                     /locus_tag="ACGI57_003608"
     CDS             complement(1447..1860)
                     /locus_tag="ACGI57_003608"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9718652.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          735 a          331 c          235 g          809 t
ORIGIN      
        1 ttagattata tattttaata tactttctta tataagaatt attttaaatc aagattatct
       61 agtattttta aaaatatttt tacagcagta tccaagtcat ctacagagac actttcatta
      121 ggattatgac ttacgccccc agcgcatctt ataaatacca taccaatatc agttatatta
      181 tccatttcct gagcatcatg gccagcacca ctataaatat aaaaaggatt aagatttaaa
      241 tctataaaac ttttttctat gatttttgtt attttattag aacatggtac aggaacattt
      301 tcaaaagcaa gttcacttgt ataattaagt tttctatttt cacatatatg tgatatttca
      361 ttaaatatct tttctacaga attatcaaga atttcttgtg aacaagagcg tacatcgagt
      421 gtaaattcag ctctttcagg taccacattt gatgaagatg gatagaaatt catctttcct
      481 acagtagcaa ccaaatcagt agtcgtttta gctacttttt cgatagctaa tacacattca
      541 caggcacaac atccagcatc aagtctcata ttcattggaa gtgtaccagc atgtccagac
      601 ttaccattta ctgatatctt aaaactttta aatcctgtaa tacttgatac aattccagca
      661 ggaagagatt cattttctaa aacaggaccc tgctcaatat gaaattctaa atatgcatct
      721 acatcttttc taggatgcaa atttgttaag ttttttgtat taaatccaaa ctttgttaca
      781 gcttcttcaa gtgtaattcc attttcatca acagatttaa gaagttcctc tttaaaagta
      841 ccagcaaaaa ctttacttcc cattaaagaa gtttcaaatc tagttccttc ctcttcagcg
      901 aaagcaatta gcttaatatt gaaaggataa gacctattat tgtgttttgc ttcttcaaga
      961 ccaactagag gcactattat acctaacata ccatcaaata tacctccaca ctttacacta
     1021 tcttgatgag atgcaactac taatgtggga gcatttggaa agcttgattt atatgtacct
     1081 ataacgtttc caatgtcatc aatttcaata tcaagattta aaccttccat ccatgaacta
     1141 attaaatcaa tagatttttt atgttcttga gtcaagataa gtcttgtaag accatttgtt
     1201 gttgaactta tttttcctaa tgtttgtaga taatccatac atttttttcc taacatattt
     1261 acctcctaaa aatataattt ttatttaagc taagattagc ttttatactt ataatttttg
     1321 aactttcaaa taattttgta tgtatagtaa aatactattt tagtattaat aatgatataa
     1381 aaacataaag tctgtagacg aagagcagaa gttttaattt aaaaatatga aatatgattt
     1441 ttattattat agtatttttc tataataata tctctttcca gttagaggat aattttcaag
     1501 tgaaaatact ttatggtatc caaatttctc ataaaattta ggtgcttgaa aactgaatgt
     1561 atctaaaaaa acatatttac aacctcgttc tttagcaatt ttttcagcct tatataaaag
     1621 ttgacttcca gtacctttat atctaagctt ctcactaacc cataagtatt ttatagaaag
     1681 ccaattacca tgagtaacac ctataaaacc tgcaacaatt ttacctgctt tatctcgaat
     1741 atatattcct aaatccactg ggtttttatc ttcaagtcta gcaagattat attttaataa
     1801 tccttgaaaa atagtatctt catcttgttt ttctattttt tctgtaattt ctaattccat
     1861 atttgaactt tctccttgta taatcttttg tatattggta ttttttaata aaatcagtag
     1921 aaagaatata gtttaacatt attctaatag gcatttatgc taatgtcaaa ctaataatac
     1981 ttattcttaa ttttatattg ttgatattaa atttttttaa ataaaaatgt agtttaagtt
     2041 atataaaaag aaatcatcaa taaatttagt aaacctaaat tgttgaatat taaaataagt
     2101 taatttaata
//
LOCUS       DAYAOX010000342         2067 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.347, whole genome shotgun sequence.
ACCESSION   DAYAOX010000342 DAYAOX010000000
VERSION     DAYAOX010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.347"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            216..482
                     /gene="rpsT"
                     /locus_tag="ACGI57_003609"
     CDS             216..482
                     /gene="rpsT"
                     /locus_tag="ACGI57_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFG9718653.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(669..1712)
                     /gene="holA"
                     /locus_tag="ACGI57_003610"
     CDS             complement(669..1712)
                     /gene="holA"
                     /locus_tag="ACGI57_003610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFG9718654.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(1666..>2067)
                     /locus_tag="ACGI57_003611"
     CDS             complement(1666..>2067)
                     /locus_tag="ACGI57_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFG9718655.1"
                     /translation="LTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPFVGVIMYLSIA
                     SLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKFYIVVIYYIGV
                     FFYIFYIEGKEIKEQENELQGYYKECKREKL"
BASE COUNT          758 a          303 c          199 g          807 t
ORIGIN      
        1 taatttatat atgtcaaaat atttaaaaca caaaatatac caatttaaaa acaagatatt
       61 gcaaattatt tatacacaat ttacagtcta aaaaatcaaa ataaaaaaca ttattaaaat
      121 atataaataa taatccttga tttttagatt tatttctgtt aaaatctaat atgttgacga
      181 tgaatatgat aaaaaaatac agggaggtga acacggtggc aaatataaaa tcagctaaga
      241 aaagaataag tgttatcgaa aagaaaactg ctttaaatag agttagaaaa tctcaaataa
      301 aaactgcaat aagaagattc gaagatgctg ttgcagctgg aaacagagag gatgctgttg
      361 ctaagttcca atacgctcaa aaaagaatat accaagtagc ttctaaagga actatacata
      421 agaatgctgc agctagaaaa gtagctaaat tagctcaaaa attaaacggt atgaacgctt
      481 aattaccagt ttatatttct tatatataat aacccctatg taggggtttt ttattttgta
      541 aatttataca ataaaaaata aatagttaga catattatag ttgattgtat atcaaactta
      601 atttagctta catctactct gaactataac atatcttgct atttattttt tattaatata
      661 tagatttttt aaattgcttc tcttttacag tatctactaa ccaacatttc aactgctaaa
      721 gtatctctta taagacctgt ctttatttta taatcacttt ctaatatata gtttaatatt
      781 tccacaatta tatcatcaga aaaattttta gtttgcttta atgcttttcc tacaacaaat
      841 tgatgcattt ttaatttatc agctataagt tttgttgaat atccatcaag ttgtaactgt
      901 cttacttgca taattatttt aaattgtctt gctatcatag aaaatatacc taatacagat
      961 tcgccttctt gtatcatatc atttaatatt ttcattgcat tagaagcatt ttgttctcca
     1021 atataatcga ttaatttgaa tatatcattc tcaacttttt tttgagataa tttatcaatt
     1081 acatcattgg ttacattatt ccctttacca acaaatgaac ttattttatt tatttcattt
     1141 tctaaatcag ataatgtctt ttcactactc ttatctctgt atccttcttg ttcaataaaa
     1201 tacatgattt gagaattatc tattataaca tcatttaaag caaaagattt ttttatccat
     1261 ttaaacaaat ccatatcaga aagcttatca caatcaaaaa caattccatt gtttcctatt
     1321 ttcttaacta atgattttct tttgtctaca tctccatata caacaaaaac tatagttgtg
     1381 gtgtctggaa tattgtctaa atgctctatt aagtatttct catcactatc tgtaaaattc
     1441 tttttctttc cttttaatag ttcaaagtct tttattatta ctatctttct atcatccata
     1501 aatggaagtg tttctataga gcttataagc tgatttaaga ctatttcctt cccatctatt
     1561 atatctaagt taaaatcaag cattccctcg tttagactat ctttaaatgc ttttattgca
     1621 ttctcaatta aataatactc tcttccataa aaaagataca ttttttcaaa gtttttctct
     1681 tttacattcc ttataatatc cttgtaattc attctcttgc tcctttatct cttttccttc
     1741 tatgtaaaat atgtaaaaaa acactcctat gtaataaata acaacaatat aaaatttagg
     1801 attttctata ttaatgtatg caaaacttaa attgctgatt ttttctagta aaaaaaatat
     1861 gctttctata aatgtactat taaaaaaaga tataatttta gctattacta cacttacttt
     1921 aaatacgatt aaacttgcaa tacttaaata cattattact cccacaaatg gtactataat
     1981 taaatttcct ataattgaaa gcaatggtat acctttaaat gtataataaa ttataggtaa
     2041 agttaatata tttgatgata ttgtcaa
//
LOCUS       DAYAOX010000343         2026 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.348, whole genome shotgun sequence.
ACCESSION   DAYAOX010000343 DAYAOX010000000
VERSION     DAYAOX010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.348"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            113..1732
                     /locus_tag="ACGI57_003612"
     CDS             113..1732
                     /locus_tag="ACGI57_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFG9718656.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
BASE COUNT          739 a          248 c          312 g          727 t
ORIGIN      
        1 atatttttat tgacaaaatt tgttattcta tattatggaa acaataaatt ttagttaata
       61 aagtttataa ttcagataaa aaaattatta atttcttaag ggaggaaata tcgtgaatat
      121 agtaattagc cttatcggtg gacttggact ttttctttac ggaatgagtt taatgggaga
      181 aggtttacaa aaatcagcag gagacaaatt aaaaaaaatt attgaactct taacaagtaa
      241 tgttgttatg ggtgtactgg taggtacagt cgttacaggt attatacaaa gttcaagtgc
      301 tactactgtt atggttgtag gatttgtaaa tgcaggtata atgaatctaa gtcaagctat
      361 tggtgtaata atgggggcaa atataggtac tacagttact gctcaattag tttcatttaa
      421 tcttgaagga attgcaccaa ttgctttagg tataggtatt ttattctatt tatttacatc
      481 aaatcaaaaa acaaagcatt tatctgagat acttataggt ttcggtatat tatttacagg
      541 aatggagttt atgaaagatg cagtagctcc acttgctgaa tataaagcat tcactgatgc
      601 acttttatat tttagtaaaa atcctgtatt aggtatattg gcaggatttg ccataactgg
      661 tattgttcaa agttcaagtg catctatggg tatgttaata gcactagctt ctcaaggaat
      721 acttcctcta tcatctgctt tacctattct ttatggagat aatataggta cttgtgttac
      781 ttcactttta tcaagtgttg gtgcaagtag aaatgctcga cgtgcagctg taatgcattt
      841 aagttttaat gtaattggta caataatatt tatgcttgtt ttaaataaac ctatatcagc
      901 catagttact cattttgacc caactgatac agctagacaa atagcaaatg cacatacttt
      961 atttaatctt acaaatgtta taatattatt accattttct aaatacatag ttaaattagc
     1021 aaatagactt atacctatta aagaaactga atcagaaata gtaaataata ctaagtactt
     1081 agatgaaagg atgtttagca ctccatctat agcacttggt aatactgtac aagaagttgt
     1141 aagaatgggt cataaagcta caaactcttt ggaacattca attgcaggat ttttaaataa
     1201 gagcaatgaa gatattaata aagcttttga gtcagaaaaa gttgtcaata agttacagaa
     1261 agatatattg aactatttgt taaaactttc taaagaacct cttagagatg atgaacgctt
     1321 tagaacagat ttgcttttta atactgtaaa tgacattgaa agagtctctg accatgcaga
     1381 aaatatagct gaattggcca tgagtgttaa ggaaatgaac attagttttt ctgatagtgc
     1441 gataagagaa atatacgaaa tatacaataa gacaattact aattttaagg atgccttaat
     1501 agttttagat gttaaagatt ttgagttagc aaataaagtt ttagaagttg aaaatgaagt
     1561 aaattattta gaaaaaactt ttagaaattc acatatgatt agactaaata atggaagttg
     1621 tacaattgat gctggtgtac tttatcttga cttacttact aatctagaaa gaatttctga
     1681 ccactcaaca aatatagtaa aacaagtact aaaacttaaa caaaaaatat agtgattatt
     1741 tttatatata atcttaatca atgagaagta agaagctata gataatgctt atatcattta
     1801 tggcttctta ttttttatgt aatactcaaa tttttatagt attctcttat tattaaacag
     1861 tttctgccta gaatattcca tatatatatg acttctttca taaaacttga actgcccata
     1921 aataaagtca caagtataca taactatttt ttaatcaatt ataaccaata tttgttttat
     1981 ttatcttcat tttaattctt cattcaataa tgtaataata ttattt
//
LOCUS       DAYAOX010000344         1938 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.349, whole genome shotgun sequence.
ACCESSION   DAYAOX010000344 DAYAOX010000000
VERSION     DAYAOX010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.349"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(364..564)
                     /locus_tag="ACGI57_003613"
     CDS             complement(364..564)
                     /locus_tag="ACGI57_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFG9718657.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(790..966)
                     /locus_tag="ACGI57_003614"
     CDS             complement(790..966)
                     /locus_tag="ACGI57_003614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718658.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1021..1118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1344..1526
                     /locus_tag="ACGI57_003615"
     CDS             1344..1526
                     /locus_tag="ACGI57_003615"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9718659.1"
                     /translation="MLNKKLPDTELKIMKCIWHTGYKTVISKDVADEIEKTYGWKHTT
                     TIILLARLTKKGFLIS"
BASE COUNT          789 a          250 c          237 g          662 t
ORIGIN      
        1 tttttattac attaaaagca attctcctaa aaacacaaat aattgtcaca acacacagtc
       61 ctcatatttt acaaattgat tctaaagaag aaataattgt gttagatatg gatgaaagtg
      121 ataatgtata taaaaaatag ttagaacttg gagaatacgg ggtattaggc tggaccaatg
      181 aaggtttata tttacattaa gagcactctt tttataggag tgcttatttt tttgaaattc
      241 attagcatat aaactatcaa gaacattact caatatacct tatttacttc accaatgatt
      301 atcttacata tattaacaat aaaaaaagac tctaaaaaga gtctttcccg aaaaatctat
      361 atattataat atagttatat tttctgcttg aggacctcta gcacctttaa ctatatcaaa
      421 gcttacttgt tgaccttctt ctaatgattt aaatcctgaa gtttgtatag ctgagaaatg
      481 agcaaacaca tcatctctac cttccattga tataaaaccg aatccttttt cattattaaa
      541 ccatttcact attccattat tcattaaaaa tactcctatt aacctatatc aagatatact
      601 tgatttaatt gtgtattacc acaaatacta ttgtatcata gattaataga aatgtctact
      661 taattatatt ttttagttaa cattttaatt attcttaata ttaaaaatat atttaaacgt
      721 tttacattgc tatatattaa tataaatgta gataaaacta aaaagggttt tctagaaatc
      781 aatgtctatc taattatact tacttaccaa taccattact tggtgtaaac aagactctac
      841 tacattttct ccgtactcta atataaaatc atctaactgt gtatctaaaa aattacaata
      901 atcatacgaa ctgctaatat aagcagtcaa tacatgtgta catttatcca ttttatttcc
      961 aaacatataa attattatcc ccctaaaaaa ctttcataaa aacaaaaaga acactatctc
     1021 tttggcaact ggctgacata actcataaga tatttatata agtcttagtc cctatagctt
     1081 tgcgtcacta aatttctcta gttttgccga tttagtttta ttctacaact aaaataatac
     1141 aatagaatta gttattattc aacaggattg tttgaaagtt aaataaattt atagatatga
     1201 acattatttt acacttaaaa acagactaaa cacgtagagt acctggtaaa aattaaatat
     1261 agtttttgtt ctaaaaagct cctattataa tttaagctag ataaatttga agaagtgaca
     1321 taaattgaaa gaaggaatat attatgctaa ataaaaaatt accagataca gaattaaaaa
     1381 ttatgaaatg catatggcat actggttata aaactgtaat atcaaaagat gttgcagatg
     1441 aaatagaaaa aacatatggt tggaagcata caactacaat tatactttta gctagattaa
     1501 ccaaaaaagg ttttttaata tcatagagaa ttgggaggca tgtacactat acagtattaa
     1561 taaaagagag agaatatctc aaaatagagg gaaaaagagt ttttagaggt ttacataaca
     1621 atcctttatc agaattaatt tcaaaattac atgatggaga agaaataact gaagaaaaaa
     1681 taatagaaat aggaagttgg ataaaaagct ggaaatataa agattaataa ataaaaggta
     1741 gctaataata taagtgctac cttttttatg tggataatat tggtgataag ttgtggaaaa
     1801 cttatataaa aattgtggat aaagtgtttt gctgacattt tactgacaaa agttaaaaaa
     1861 ataaatgtta tatattgtaa aatgttgaaa aatagccata tatataaaat ataagtaaca
     1921 ctatagataa attactgt
//
LOCUS       DAYAOX010000345         1921 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.350, whole genome shotgun sequence.
ACCESSION   DAYAOX010000345 DAYAOX010000000
VERSION     DAYAOX010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.350"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..935
                     /locus_tag="ACGI57_003616"
     CDS             <1..935
                     /locus_tag="ACGI57_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9718660.1"
                     /translation="IYLIFSFFRKSIKLISQDKRNYVYLLFALLVNTMNMLVNYVLKK
                     LERFGSLHSEGYYFDNFVNPKLVGASSIFLILLFKEIIKENRLKSQAELIKNKLDMQY
                     AHYLSIQESHMRVKKLYHDINNHIYCIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTG
                     NMILDIIINEKSEVCLKKGIKFTCSINFSKVNFVKPIDVSSIFSNILDNAIEACDKIV
                     DENINKYIRIKGTITRSFFVLKCENSKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSS
                     LQKYNGELLFENSIDKFILNIYIPLDQDTDSWGD"
     gene            1176..1865
                     /locus_tag="ACGI57_003617"
     CDS             1176..1865
                     /locus_tag="ACGI57_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9718661.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
BASE COUNT          805 a          173 c          302 g          641 t
ORIGIN      
        1 ttatatatct tatatttagc ttttttagaa agtctataaa attaattagc caagataaga
       61 gaaattatgt atatttatta tttgccttat tagttaatac aatgaatatg cttgtaaatt
      121 atgtattaaa aaaattagaa cggtttgggt cattacattc agaaggatac tattttgata
      181 attttgttaa tccaaaattg gtaggagcta gtagtatatt tcttatacta ttgtttaaag
      241 aaataataaa agagaataga ttaaaatctc aagctgaatt aataaaaaat aagttagata
      301 tgcaatatgc gcattattta agtattcaag aatctcatat gcgagttaag aaactatatc
      361 acgatataaa taatcacatt tattgtatag acaaccttag aaacaatagc aaagaaatta
      421 atgaatatgt taacaattta aaagatgaaa taaaaacatt taaatatatc tataatacag
      481 gaaatatgat attagacatt ataatcaacg aaaagagtga agtatgttta aaaaaaggaa
      541 ttaaatttac ttgctctata aatttttcaa aagtaaattt tgtaaaacct atagatgtgt
      601 caagcatatt ttctaatatt ttggataatg caatagaagc atgtgataaa atagtggatg
      661 aaaatattaa taaatatatt agaattaaag gaactataac aaggtcattt tttgtactta
      721 aatgtgaaaa tagtaaacta aatcagctta cttttaataa aaatatattg ttaacaaaca
      781 aaatggataa gttcgtacat ggaataggta ttcagagtat taaatcttct ttacaaaaat
      841 ataatggaga attattattt gaaaatagta tcgataaatt tatactaaat atatatatac
      901 cattggacca agatactgac agttggggcg attaagttac cagttgggac atatgtattg
      961 atacaaaaaa ataaggatgt tatcttttaa taacaaaaga taacatcctt atttctgtat
     1021 taaggagctt ataattttac taatagtaag ttataagaat tggataggaa ggtgatatgt
     1081 attgaataat aaatctttaa tataaagaaa atataaatat attctagtgc aatgaataat
     1141 actttagagc ggttagtgtg atgggaggat tgtgtatgaa agtaaaaagg aatataaata
     1201 gagtggcaat aattgtggct ttaaatctta gcttagcatt gatggtaggg tgtatgtctc
     1261 ctgtatctga taagaactta agtagctcag aacaaattag taaagattca ttgaaagaac
     1321 aagttgaaga taaaaaagat gaatctattg agctatttaa taattactca caaaagatta
     1381 atgatgcgaa aggtagagaa gaagtaaaaa aagatttatc tcaaagattt tcgaaagcta
     1441 taatagacag tggttatagt attactaact caaaatcaga tggtagcttt gtagtatcta
     1501 taaatggtgc tgaaaaacca cctgaaaaaa atattcgaag tttattctat tctatgtctg
     1561 aagacgataa agaaggaaaa gtaacaatag atattctctg tgctaaagag tatccagaag
     1621 atgataagtt atctgagagt gataaatatg ttaaatttat atacaattta tttaaatcat
     1681 tgactgatac gaacttaact gagaaagaaa ttttttctat ggttgaagaa gactttaata
     1741 agggagtagg tgttgtagag cttccttata tgaaggatat acatgttgaa gttaacaaag
     1801 ttaaacaatc aactaaggtt ttaaaactaa gtctagtata caaatttgat atttcaaatc
     1861 attagtatat tagacataac tataacaata tagaaaatat atcatagaaa atttttttag
     1921 a
//
LOCUS       DAYAOX010000346         1913 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.351, whole genome shotgun sequence.
ACCESSION   DAYAOX010000346 DAYAOX010000000
VERSION     DAYAOX010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.351"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            124..1566
                     /locus_tag="ACGI57_003618"
     CDS             124..1566
                     /locus_tag="ACGI57_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9718662.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1608..>1913
                     /locus_tag="ACGI57_003619"
     CDS             1608..>1913
                     /locus_tag="ACGI57_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718663.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     "
BASE COUNT          716 a          212 c          361 g          624 t
ORIGIN      
        1 ttgtaattta tgatataata acaaatatat aattattaaa ttataataaa tgactatagc
       61 gtagatgtaa tactatgtta tagtcatttt tgaattaaac ttgatatatc aagggggaag
      121 aatatgagaa caattagaag agttttagca ttaggtttaa cattagctat atttctaata
      181 aatgtaccta atgtagatgc attaacatca gatacaatta aaggtaacaa tatatatgag
      241 acggctggat taatagcaga taaaaagagt tatgatacag caattatggt aaatatggat
      301 aattctatag cagatggtct ttcagcaagt ggtcttgcag gtgctgttga tgctccaatt
      361 ttgcttgcac aaaaaaataa gataccaaat gaaacaaaac aaagattaaa aaatgttaaa
      421 aagatataca taataggtaa agaattatca ataagtaagt cagtagaaac tgaactaaaa
      481 aacacagggg cacaggtaac tagattgggt ggagatgaca gaataaagac tagttatagt
      541 gttgctaaag aggtaaatgg tatcaaaaaa gttgatgaag tgatattaac taatgcatat
      601 aaaggagaag cagatacaat aagtgctgca cccgtatcag taagagatat agctcctata
      661 gtacttacag atggaaagag tgtgcctttt tcaacaagca atgtaaaaac atatgcagta
      721 ggtgggagta tatcaatgag cactagttta gttaataaaa ctaatgctaa aagacttggt
      781 ggttcagata gatatgatac aaataagaaa gttataaaag agttttatcc agatgcatca
      841 gaattttatt tgagtgatgg atatgattta gtaaatgcac ttacaggttc tacaattgct
      901 aaggaaaatc caattgtatt ggtgtcagaa agtagtgata agtctatatt agcaggagca
      961 gataagatta ctagattagg ttcaataagt gacagtgtgt ataataagtg tgtttctgct
     1021 gcacaaaata atggcgattc gtctacaaaa ggtgagtcac ctatgaaaaa tgagacaagc
     1081 atattaggtc agccaacagc tagtttagaa gcgtgcttaa aatgggcaaa atctaaaaaa
     1141 gctaatgatt tatttataga gttaatacca atattatatg atactgctgt tcaggaaggt
     1201 gttaatcctg ttttggcagt agctcaatct gcaaaagaaa ctggtttttg taattttggt
     1261 ggagtattag atgcatcatt taagaatcct tgtggactta aaacttctgt aggtggttct
     1321 gatactgata agaatgccca ttcaagattt gatacttggg aagaaggaat attagctcaa
     1381 attcaacatt tatgcttata tgcaggacaa gatggttacc cactttcaaa tccagtagac
     1441 cctagacatg aaaaatcttt atttggtaag gctaaaacag ttgaaagttt atctaataat
     1501 tgggctggag gtcaatatgg acaagattta gtaagaatga tgggagaaat tgaggcaaca
     1561 aaataattga agattatcaa atatccaata aataaggatt gattattatg aaaaagagta
     1621 atatttttag tatcatattt cctacagtca taatggtgct aatgttttta acttttgcta
     1681 cagatatatt taatattcca gatattcaat ctaaaagtat ttttgttgtg ggaatggttt
     1741 tgatttttcc aatttctttt ttgatacaag ggataatttg tgtattaaat aagactaatt
     1801 ggattttatc tcttattgtt tctttagtaa cttatatagt attaatgtat atatttttaa
     1861 atgattctgc atatatatat gttttacttt atacagcttt ttatatgatt gga
//
LOCUS       DAYAOX010000347         1876 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.352, whole genome shotgun sequence.
ACCESSION   DAYAOX010000347 DAYAOX010000000
VERSION     DAYAOX010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.352"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            315..1025
                     /gene="rgaR"
                     /locus_tag="ACGI57_003620"
     CDS             315..1025
                     /gene="rgaR"
                     /locus_tag="ACGI57_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HFG9718664.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          740 a          145 c          267 g          724 t
ORIGIN      
        1 ttttatttta tgttgtgaat atatgaattt tatgtcttaa aatttataag caataaaaat
       61 ttgatatatt atgatgttta atatagggta ataaatacat attatattta ttttactata
      121 tttttttaca taatatatct agaaaagtat aggaatttat cgtaatttaa gtttaaatat
      181 taaagagaat ttctataagt agtttaatcg acaatttaaa tttataaaga aatttaaatt
      241 tgaaagctag tgagtacagt ttggaaattg aaataaagta aactaaatga tattttagat
      301 ttggaggtga tattatgttc agggtagtta tatgtgatga cgaaaaaata caaagaagca
      361 tactaaaaga gtttacagaa aaacttttgt ctgaaagatg tctaaattat caaatactgg
      421 aattttcctg tggagaagat ttaatatcca aatacccaga aaaaattgat attatatttt
      481 tagatatcca aatgaaagat atcaatggaa ttgaggtggc taaaaaaata agaaagtttg
      541 atgaaaaagt agaaattata tttacaacgg ctttttcaga gtatgccccc caaggttatg
      601 aggttcgtgc ttatagatat ttagtaaagc ctatagagta tagtaacttt gcaatgggtg
      661 taaatctttg tattgataac cttcaaagaa agaaggagcg ttatatagtt ttaaatagta
      721 aaaaaggatt taatagaata ctgatagatt ctattttata tgttgaaact gtaaagagag
      781 atttagtcgt acatacaata ggtagagatt ataaagctaa tatgagcatg aaacaagcag
      841 aaaaattact gtgtgaaaat ggctttttta gatgtcatac aagttttatt gttaatttaa
      901 gaagaataga agatcttagg gataatatga ttactattaa tgaacaattt atacctataa
      961 gtaagtatag acttaaagat ttaaaagtag cattaacaag cttgttatgt gatataatgt
     1021 attaatttta aaaaggtaat tgaaaatatc gtaaaaaaga tttaatgtca tcacagggct
     1081 attgattgaa actgtcttaa tatataaaat gttatttata ttaagacagt tttattatgc
     1141 aaaatagcag tttatagatt tttaatatgg tttaacatgt atatgtagag gttccattat
     1201 agtagcatga ttattaatta gtagcttgat tatcaatata ctattattga tttttatatg
     1261 cattatatgt atttttagaa tatgattacc tttttaaaat aatgattatt tataaaactc
     1321 tatagatatt tgtttcattt agctacgcat actttaatta attatattaa tatttaatgg
     1381 agtacaatat aaatattaaa gcgacatctt atagtttatt gaaactatag taatgaatta
     1441 ataaatagtt taattctatg aaaagatttc cttttatgca atttatttat tgaaatatga
     1501 tataatatat aaaggtgtaa gtattagttg ttaaaataat atttatatta gtttttatac
     1561 atacttggta taagtttttc atatattgat taaaattgat gttgatttat tatttttagt
     1621 attttttcac cttttgattc atactttgta gaatggtgta ttttctaaaa ctgattataa
     1681 gacaagttca ttcaaattag catttttata tcatataaat aattgttccc cctaaataag
     1741 atttagatgt atagtattaa aatcaaaaag tataaaataa aattgatgct taatcataga
     1801 taaatattaa taaaatatac aaggggattg tctaataaaa agataatctc cttttttaca
     1861 aataattcta aattgt
//
LOCUS       DAYAOX010000348         1852 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.353, whole genome shotgun sequence.
ACCESSION   DAYAOX010000348 DAYAOX010000000
VERSION     DAYAOX010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.353"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            102..386
                     /locus_tag="ACGI57_003621"
     CDS             102..386
                     /locus_tag="ACGI57_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718665.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(1157..1372)
                     /locus_tag="ACGI57_003622"
     CDS             complement(1157..1372)
                     /locus_tag="ACGI57_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9718666.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1383..>1852)
                     /locus_tag="ACGI57_003623"
     CDS             complement(1383..>1852)
                     /locus_tag="ACGI57_003623"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFG9718667.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMIISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          751 a          198 c          234 g          669 t
ORIGIN      
        1 tgttttatat aaagtaattt gaagaatatg gaaaaacttg attagtacat tattaattag
       61 ggtataaaat ggtgtaaata gaatgataag gggtggatga aatgcttgta tataataaaa
      121 gtttttatcc taatgacata tttccaagat tagatttttc aaaaataaaa aaacagttaa
      181 aattgataga taatgacctg tcagattttg gaagcatatg tataatagaa aaagaacatt
      241 atacaataag tgtaaacagt ataggtgaaa taaatgtgta ttatgattta gagtacgaaa
      301 ataaggtgta tagaatagtt tatgagattg aaaagttatt taaatctcaa gttggaaggt
      361 ttagcatatc tacatacaga aattgataat taaaaaagta gaaattaaaa aacttaatac
      421 taaagatata gataaatata tgaggtaaca gctagagaga gttgttaccc tttttaaata
      481 ctaagtatat attattctta ttaatttttt gtaaattcac taaaagacct ttttatagaa
      541 tattattttc tttttttatt aaataggtaa atcagtaaaa atgcaattcc agttccaatt
      601 attattccaa tgatattttc tataattaat ttttttaata taaaagtaaa taataaaaat
      661 gctatacata aacctaaaat atatccttta aaataggttg aaagttttct tttcatataa
      721 tcaccaactt gtatcagatt tagtttactt aattagagta taacagtgtt gtaacttcaa
      781 agagtatcta aaagattaca aatgttagat aaaatcatca ataatgtaat ttataacacg
      841 tagctataaa ctatgcaata atataaatgg aggtataagt aaatgaaaaa gttccatgtt
      901 ttgctaacag tttaaataaa tattaggtag aaaagaatgt atataggaat ctgtggtaaa
      961 attatcaatt taaaagtatt atttagtgtg atacagttct tataggtaat ggataacaaa
     1021 tagataaagg aacagtttct ttcttagaac atattttgtt atgactttaa acaaagttaa
     1081 tattaatgtc aataatatag taaaagaata aaaatcatat agtataataa aaaagagtgt
     1141 ttctaaacac tcttttttaa tcattttcat cttctacata ttcaagtata tcacctggct
     1201 gacagtctaa agctttgcaa atttcgttta gagtggtaaa tctaatagct tttgcttttc
     1261 cagtttttaa aattgataaa tttgcatttg taatatctat tttttctgcc aaatctttaa
     1321 gtgatatttt cctttttgcc atcattacat ctaggttaac tactattgcc ataacatcac
     1381 ctctatatcg ttaaatcatt atcttctttt atttttatag ctttatcaaa tgtttctgca
     1441 attacaaagc acattagtgc aaatataaaa gagataatca taagtggtga gataccgcca
     1501 ctatcagttc caaaaattct tatattatta gtatttaaat tataaccatt gatacattca
     1561 attatagtgt ttatcaataa acaaaatccc attaccttaa acctttttac attttcaagt
     1621 acgaatgggg aatacttagt actattaatt ataaatatca agaataagat gactataaag
     1681 tagtcagaaa aatatagtat tcttgcaatt aaagcgatta ctttgtcaga actaaaatca
     1741 tataaaaaca taggtatcat acataagaat attataccag caactcctaa aagtgataat
     1801 agaaaatata gaaatactga taatatatta ctaaaaaatt ttgtaatcat aa
//
LOCUS       DAYAOX010000349         1846 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.354, whole genome shotgun sequence.
ACCESSION   DAYAOX010000349 DAYAOX010000000
VERSION     DAYAOX010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.354"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(109..324)
                     /locus_tag="ACGI57_003624"
     CDS             complement(109..324)
                     /locus_tag="ACGI57_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718668.1"
                     /translation="MHINQTGFISNELGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVRGGSSWAFNFIVQP"
     gene            complement(507..851)
                     /locus_tag="ACGI57_003625"
     CDS             complement(507..851)
                     /locus_tag="ACGI57_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718669.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            978..1361
                     /locus_tag="ACGI57_003626"
     CDS             978..1361
                     /locus_tag="ACGI57_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718670.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
BASE COUNT          689 a          234 c          230 g          693 t
ORIGIN      
        1 ttgttactat aacataggaa ttatctttaa aaacacaaat taatttacat tctctaattt
       61 taaagtatat gtatatcttt gattttaata aaattcactt aaaatatttt aaggttgaac
      121 aatgaaattg aatgcccaag aacttcctcc tcttactaat gttgcaatta attcaaaccc
      181 acaattgtat tgaactgaat ctctacctac tggtttccaa ctagaaggtg cattatatga
      241 gaaagaattt gatgttggct ctttcttttg ttgctgctta attcctagct cattagaaat
      301 aaatccagtt tgatttatat gcacccttct atttgttaca cgaatctggc cgtctgcaat
      361 agaccagttt ccagaaactt tactaacgga aatatattga aatgctccat ctccttttga
      421 tattgtatat gtaactgtac cagatgcttt tacaccacca gttttatccc atccatcttc
      481 tgatatactt gcttttctaa gaggctttat tgtattagta tcaaaaaaac tgtcttagaa
      541 tatatctttg atgttaaatt tttagattgt ttaacttcta ataattgagt tgttgatata
      601 gaatcaacgt atatggactc tcctgttctt gcatttttga ttataccttt ttcattggaa
      661 cttttaacat cagtaattcc atttttagcc atatcgtata atttgtccat gtctgttact
      721 tgtttctctg aatacattac tttcatagat gaatcttcta ccttactagc acttgcattt
      781 gttggtaata gtattgtcat tatgagtaaa gataaaatag ttgttacaga tttttttaac
      841 ctataattca tattaatcct tactttttat ttgttataac agacattcta ttgtaataga
      901 atataaatcc acaatatttt taaaattagt attgaataac tattctcatg ttataattat
      961 tcaaaggagg gattgatatg aggaaaaaat tttttatagg tattcttata gttttaataa
     1021 tctctgtgat agctatactt aatataaaaa atgataaaaa tgtctttatt ggagaatcag
     1081 aaaattggaa agctaaattt gtaactactg aaggacactc aaaattagaa ataaaatata
     1141 aaaatagtaa agatgtagtt gaacaaaaat ttaattattc ctatcaattt atagatggtg
     1201 aagatgcagt tgtagttgta aaaaatgaaa ttttagaagc aggtcaagaa aaagcaataa
     1261 aaatacaaag accaggtctt tattcaaata gagaatctag ttacaagcta attatatctt
     1321 ggaatggaaa tgaggaaact ataaacttga aaaagaaata accatattat caatttttaa
     1381 aaaattatta ttcaaattct tataaaaaga gtgtttagaa agatgaaaca ctcttttttg
     1441 tataagtttt tgaaaactat aaatgaaata tatcaaaatc gttcgaaaaa ttactgaaat
     1501 aaactcttaa atatattata aattaaggta tattatacat gaatttattg ggagtttatg
     1561 agttttaaaa ttggaaacca aacaataagg gcgaaaaata cttcaaggaa aatatataaa
     1621 gttctataaa ttatcaaaaa tatttcgcaa aaattgataa ttattacaaa taaattgata
     1681 atattagaaa tatcgaaatt gaacaaacta ggattaaagc tcaattagaa aaatccacta
     1741 atcctgaaaa tactttgtta ttctcattta ttactgttgt tttttcatta tttgctaatt
     1801 tatttttcag ttcattaaat gttaaatata actctataaa cttaat
//
LOCUS       DAYAOX010000350         1843 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.355, whole genome shotgun sequence.
ACCESSION   DAYAOX010000350 DAYAOX010000000
VERSION     DAYAOX010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.355"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            18..392
                     /locus_tag="ACGI57_003627"
     CDS             18..392
                     /locus_tag="ACGI57_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9718671.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            389..1252
                     /locus_tag="ACGI57_003628"
     CDS             389..1252
                     /locus_tag="ACGI57_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718672.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1252..>1843
                     /locus_tag="ACGI57_003629"
     CDS             1252..>1843
                     /locus_tag="ACGI57_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFG9718673.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFV"
BASE COUNT          697 a          196 c          294 g          656 t
ORIGIN      
        1 tttaaggagg tgtcttattg aatatagtta ttagtaattc gagtgggaag ccaatatatg
       61 aacagattac tacacaaatt aaaagtatga ttataagtgg agagttacca gaaggctcag
      121 ctcttccaag tatgagacta ttggcaaagg aacttagaat aagtgtaatt actacaaaac
      181 gagcatatag cgatttagaa cgagacggat ttattgagac agttactggt aaaggcagtt
      241 ttgtatctag taaaaacatg gattttgtca aagaagaaca atttcgttta attgaagaat
      301 acctacaaaa agcagttgat acagcaaaaa gtagtgatat tacatatgac gaattaaaag
      361 atattttatt tttattatat aaaggagaat gataagtatg gatgcaataa ctataaaaaa
      421 tttaaataaa acttataaag attttagcct acaagatata tctttttcag taccaaaagg
      481 gagtgtaatg ggatttgtag gtgaaaatgg tgcaggaaaa acaactacat taaaagcaat
      541 tttaaattta atatcttatg atagtggaaa tatagaaatc tttggtcttg atagtaaaaa
      601 aaatgaaaaa gaaattaaag agcaaattgg ggttgtattt gagggaagca actttcatga
      661 aaatttaaac actgaccatg tttcaaaaat catgagtaaa atctataaaa attggaatga
      721 cacattattt aaagactact taaaaaaatt gagagtacca gacaataaat taattaagga
      781 gttttcaaag gggaataaga tgaaacttag tattgctgta gccttatctc ataaaccaaa
      841 attattaata ctagatgagg caactagttc acttgaccct atcgttcgtg aagaaatact
      901 agatatattt ttagatttta tacaagatga agaacactct attatcttat cttcacatat
      961 tacaagtgat ttagataaga tagcagacta tattacattt atacataaag gaaaaattgt
     1021 atttagtgaa aataaggatg aattaattga tactatggga gttttaaaat gtaaacctag
     1081 tgattttgat aacctttcaa gagaggatta ttcatattat cgtaaaagcc aatttggata
     1141 tgaagtactt cttaaagata agcatagatt tatctcaaga catccaaact gtattgttga
     1201 taatacgtct atagaagaaa ttatgttatt ttatgtgaga ggtgataaat aatgaaaggt
     1261 ctaattttaa aagatttact taatttgaaa ggaaatataa agtttatact tttattcatc
     1321 attatgtttg gctttatgtc ttctttagga gatggaaatg ttaataactt tataggtgtt
     1381 attatagttt tatgtactac aatgattgtt tcaacatttt catatgatga tttgaataaa
     1441 tgggatagtt atgtacttac aatgcctatt aatcgcaatg atattgtatt aagtaaatat
     1501 ttaactatgt taatatttag ctttataggt gttcttgttt cacttattgt tagtgtaact
     1561 attggttatt ttaaaaatac acttatatta aatgaaacac tacttattaa tgcactaatc
     1621 ttatcaattt cagtatgttt tggtagcctt attctaccac ttatttataa atttggtaca
     1681 gaaagagctc gtttattaat gattttatgc tttttagttc caacactagc tttattagtt
     1741 tttaaaagta ttttagaaaa tattagttca cctatttcta tagaaattat acttaataca
     1801 ctagtatatt ctcttccatt tgttgctatt ttactatttg tga
//
LOCUS       DAYAOX010000351         1831 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.356, whole genome shotgun sequence.
ACCESSION   DAYAOX010000351 DAYAOX010000000
VERSION     DAYAOX010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.356"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            68..1768
                     /locus_tag="ACGI57_003630"
     CDS             68..1768
                     /locus_tag="ACGI57_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718674.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
BASE COUNT          707 a          211 c          352 g          561 t
ORIGIN      
        1 aatatttatt ttttgtaaat atatggatat aatagtaata ataacatttt ttagagagga
       61 gtattaaatg aaacgatatc ttaaaacata tggagttgca ttaataattg taataattta
      121 tgcatttatt aaattacctg tactaagatt agattttaca tctttatttt cagtaggaat
      181 aattttcttt gttgtagcag gaatactaga tatgatgtta gatagaaatg aaagagcatc
      241 taaaatggct aaatataatt tttcaatagc aatagctttg cttgtgtata ttgtagtagt
      301 tccctttata acttctacac ctgtacttca tgcaaaatca tatagagaac tattgggaaa
      361 agtaactgaa agtaaattta ctgatgacat tagccctgtc agtgtaaatg acattcgtct
      421 tgtagatgaa gatatggcaa tgaaattagg tgacaaaaag cttggagaag ttcctgcaat
      481 agggagtgta tccaaattag gaaaatttca tatacaaaat gtagatggag agttgtactg
      541 ggtagcgcct cttgttcaca gagacataat taaatggata acatctctaa gtggtacaag
      601 tggttatgta aaagtatcag ctagtaatcc tcaagacgtc caattagttc aagaaataga
      661 tggaaagcca ataaagatag tttaccaacc agaagcctac ttacatcagg atttacaaag
      721 acatttatac atacatggta ttgtaaatgt tggtatgaca gatttcactc ttgaaataaa
      781 tgatgaagga agaccttatt gggttgtaag tttatatgaa cataaaattg gctatggagg
      841 agcaaatgca acaggaatag ccactgttga tgcagaaact ggaaaaataa atgtttatga
      901 tgttaaaaac acgcctaaat gggtggatag aatacaacca caaagctttg taacagacca
      961 aattaaagac tggggtgttt atgttaatgg atttttaaac tctgttatat cagaaaaggg
     1021 agttttagta ccaacagaag gtacatcatt agtttatggc aatgataaca ggtcttattg
     1081 gtatacaggt ataacatctt caggtggaga tgaatctaca attggattta tgttagttga
     1141 ttctagaaca aaagaagcta gattatataa acaaccagga gctactgaaa cggctgcaat
     1201 gaaatcagct gaaggtaatg ttcaagaaaa aaattatgaa gcgacgttcc ctgtaatgta
     1261 taatatattg ggtcagccaa catatgtatc atcattaaaa gataaggcgg gtcttgttaa
     1321 gagagttgca tttgtatctg ttgaagatta taatgtactt ggaataggtg aagataagaa
     1381 tgaagcatta agaaactata aggatgcatt agaatctaag ggtaatgatt taaaacttga
     1441 taatgattta aaagatgagg tattagaagg cacagtgaca agaataagcc cagatgtgcg
     1501 aggtggtaac actaattatt atgtaacttt agattcaaat aaagatataa tatttagggc
     1561 tacttcaaaa gtatctagcg aattgccatt gactcaggtt ggtgataaag tcaaaatatc
     1621 gtattcaaaa gaagaatctg gtgtaataga aatgtcagaa tttgataacc taaatattgc
     1681 aaactttgaa gataaaaaag caaaagaaaa aacaggaact gatgaaaata cagattccaa
     1741 agattctgaa aatcaaggta atgtataatt taaataaaaa gattgataat agtatttaac
     1801 atagtgatag ctaattttta tagaatttaa t
//
LOCUS       DAYAOX010000352         1818 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.357, whole genome shotgun sequence.
ACCESSION   DAYAOX010000352 DAYAOX010000000
VERSION     DAYAOX010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.357"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(13..231)
                     /locus_tag="ACGI57_003631"
     CDS             complement(13..231)
                     /locus_tag="ACGI57_003631"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9718675.1"
                     /translation="MFFSLFKKKSGYTFTNFLSKVRVEKSKKYILKKDLSILDVAVLI
                     GFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            524..715
                     /locus_tag="ACGI57_003632"
     CDS             524..715
                     /locus_tag="ACGI57_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718676.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(804..1616)
                     /locus_tag="ACGI57_003633"
     CDS             complement(804..1616)
                     /locus_tag="ACGI57_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HFG9718677.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
BASE COUNT          681 a          244 c          207 g          686 t
ORIGIN      
        1 gaatgatatt ttttagttat aaatattttt atattcaatt ggtgtaaagt tattaaactt
       61 tttaaaaatt atactatagt aattttgact attgaatcca attagtacag caacgtctaa
      121 aatagacaag tcttttttta atatatactt tttgcttttt tcaactctaa ctttacttaa
      181 aaaattagta aaagtatatc cacttttttt cttaaataaa gaaaaaaaca acttttatta
      241 agttgtaatt cattacatat attacttact gttatatctt ctgagtaatt tttgtgaata
      301 taatctatag ttttctttaa gttttcttta cataaaacta agagttggtc tatgtttcat
      361 tttatcaagg catatttatt aaaaaatcaa tctctatttg ttaaaataat ttatgaatga
      421 tgttagaaac tgtaatatat tggatataat ataataaaca gataaactct gtattaagag
      481 gatatatagt ttatcttaat tataggggaa gtgaggatat attatggaaa gattaatgga
      541 actcatagaa agaaaatttt taaaggatgt agaactttta gaattagaag acatagaaaa
      601 tgtaatagaa tgcaaaatga cacagccata taatgctcat tataatgaat atttagtaaa
      661 agtaaaaaat ggagatgaag tagaagaata ttttgtgtat ttaaaacata gataaacata
      721 aaagaggttt atatttattt gtatgattat aaaataaata caagcctctt tttttgataa
      781 atataaaatt ataaaatttt tatttattta attttttcaa caacattagt caatattgaa
      841 tctaaatcat cactaatttc tgtaactcca ttatgagtaa tatctataat ttctttagtc
      901 ataggaagtt ctcctaaaag ctctaagtct aaatcatcta aaaacttttc agtagattct
      961 ccttcaaata acttgatttt tttagaacaa tcaggacatt ggatataact catattttca
     1021 ataactccaa ggacatttat gttcattttt ttagccatat taacagcttt tgatacaatc
     1081 atagatacca aatcttgagg tactgaaacc ataactattc cacttatagg aatagattgc
     1141 ataactgtta aggcaacatc tccagtacca ggaggcatat ctattaataa atagtctaat
     1201 tcttcccaaa gaacatcagt ataaaattgc tttacaaccc ctccaagtaa aggtcctctc
     1261 caaacaacag gttcattttc atcatctatc ataaggttta tagacattac ttttatgtta
     1321 tttgagtttt ctacagggta aatataagag ccatcagaat aagcttttac attttttaca
     1381 cccataagtc taggaatact tggtccagtt atatctgagt ctaatatacc aaccttatat
     1441 cccattttgt ttagcttatt agcaagtaaa gcagttacag ttgattttcc aactccaccc
     1501 ttcccactca taacaccaat tatttttcca aatttattgt tagggttatt ttcaatagaa
     1561 caatttgatt gactattaca atttcccttt gatggacatg aattacaatc tgccatatct
     1621 attacctcca aactttaata tttattagat tatttgtaac ctacatatac tattttagta
     1681 caattgaacc ataaagtcat tatttaaaag caacttagaa gattttctat tgatttattc
     1741 atagactata gcaaaaagaa tacaataaaa aaattataac attcttgaaa aagtgattat
     1801 ttcaatttag ttatagaa
//
LOCUS       DAYAOX010000353         1804 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.358, whole genome shotgun sequence.
ACCESSION   DAYAOX010000353 DAYAOX010000000
VERSION     DAYAOX010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.358"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..988)
                     /locus_tag="ACGI57_003634"
     CDS             complement(<1..988)
                     /locus_tag="ACGI57_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718678.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNE"
     gene            complement(975..1742)
                     /locus_tag="ACGI57_003635"
     CDS             complement(975..1742)
                     /locus_tag="ACGI57_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718679.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
BASE COUNT          639 a          306 c          161 g          698 t
ORIGIN      
        1 tttcgttatt tacataagaa tttcctaaat ttatggtata actgtattta taattttcac
       61 ttatgttcct atctatatcg taataaagtg atatggtaaa ccctagcata gttacacttg
      121 tagctattaa tattgctatt acagccaata cactactatt gctctttaat ttaaatccaa
      181 gttcagaaaa tgcaattaaa ttctgtccct tataataaaa actttttctc ttcttaacag
      241 catcaataat catatacatc gttgatgaaa aaaataaaaa agttccaggt attattgtaa
      301 ctaatataaa aactgaaagc ataatatttt cagcaataaa ataactaatt gacattaaat
      361 aagaaatagc tattaataaa acaccaagta cacattttaa ctttataaat tttttaaact
      421 tatacttttc tggatattta ttaaataact ctataattct cttatttctg ataactatag
      481 tatttcttat tccaataact atgaatatta ttaaaaatac cgctagtgtc tgaactaagg
      541 cctttatact taagttcata tttactacta tcatttcatt cattaatttt acaagcatca
      601 tagtcataag ctttgaaaat aacagtccta aaaatatacc tattagtatt gcaactatac
      661 ccatcaaaaa agtttcatat aaaaatattt ttcctatctg atttttcctc attccaagta
      721 aactataagt tcctacttct cgcctacgtc tattgaaaaa aaatgagttt gaataatata
      781 taaataaaaa tacaaatacc actattacaa ttgaacttgt attaatactc atcctagttt
      841 tctctccaag tgaactcaac gcttcattat actctataga tttaaaacta aaaaatacaa
      901 atacactaaa tacaattgaa ataaagtaca ttaaataatt aaaaaaacta ttctttacat
      961 tttgcattgc aattctaaat aaagtcattt ttattaactc ctccaattaa ggaaactgta
     1021 tttactattt tattatagaa ctctctgtta ctttctccct ttacaatttc tgtgaatatt
     1081 attccatcct ttataaagag tattctattg caaaaactcg cacttatagc atcatgagtt
     1141 accatcaata ttgttttatt gctgtctttg tttaatttta atagacaatt caacaaatcc
     1201 ctagcagatt tagaatctaa tgctcctgta ggctcatctg caagtactaa tgatggtttt
     1261 gttgctatag ctctacatac agctcctctt tgcttttgtc caccagaaac ttcgccaggg
     1321 tatttatcta atatagattc tatatttaat tcttttgaaa tctcttttat tctattttct
     1381 attactgata ctctttcatt ttttaaagaa agaggcaaca ttatattttc ttttattgta
     1441 agtgtatcca aaagattaga atcttgaaat acaaagccta gattatcacg tctaaaatct
     1501 gccaattcat tttgcttcat tttaatagta ttctttccat ttatataaat atctcctgat
     1561 gaaggtaaat ctattgtaga aataatattt aaaagtgttg atttaccagc accggaagac
     1621 cccataatac caacaaattc tcctttataa accttgaaag ttattcctct tagagcttca
     1681 tgttttgaac cttttcttcc atactccttt tttatatttt caacagataa tatttcttcc
     1741 atagttaata atcacctttc atcacaatta gttatttatc aattctcaca aatatattat
     1801 atat
//
LOCUS       DAYAOX010000354         1792 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.359, whole genome shotgun sequence.
ACCESSION   DAYAOX010000354 DAYAOX010000000
VERSION     DAYAOX010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.359"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            40..228
                     /locus_tag="ACGI57_003636"
     CDS             40..228
                     /locus_tag="ACGI57_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718680.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            complement(638..1222)
                     /locus_tag="ACGI57_003637"
     CDS             complement(638..1222)
                     /locus_tag="ACGI57_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718681.1"
                     /translation="MKGEYNKEKEKVIKFNSNKDKSKINKYTDYYNTLNSTLMEKNKC
                     IGDNWFLATTSEEVGNMNEEEKRILETEKRILETEKRVHQNNKEVKESIDKQFNDIKG
                     IFKEYKNDLDKDRIELKNDLARERIELKSDLKEAINEHKKVTEKDVSEIKNSIKGIED
                     RIDSTNKWIIALCITTIIGIATMAITIGISIWPK"
     gene            complement(1225..1716)
                     /locus_tag="ACGI57_003638"
     CDS             complement(1225..1716)
                     /locus_tag="ACGI57_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718682.1"
                     /translation="MAYENGTNLVAYFPNCIKKYEKDNKIFEKTILLLNLYMQMPTWP
                     HCIHEFKAKANEILGEDPEFIGEYGIKLKDFYNEGENVNISQNFKNDLYTFSNSTYDI
                     LDNSYIQRINPIGLYSISSLARENDFILKLFRNDKKSLELELSKSEIQSIIEHLESMI
                     EEE"
BASE COUNT          582 a          247 c          178 g          785 t
ORIGIN      
        1 acttaattat ggaaaatata aaatgaaagg aataaaaata tgactaagac tatattatgt
       61 gattactgta ataaaggaat aaataaagat gataataagt atattacttt tcataagaaa
      121 agtcatatga aaactaacat ttgtattaat tgtgcattaa atttgataga taaagataaa
      181 ttaaatgaaa atattataaa taatcaacat gattattcaa agaaatgaaa aagcactctc
      241 cataatgaag aatgctctat ataataatgt ttgacttagt aaagatgtgt tggggttaca
      301 tatttacttt tttattatat catataactt tgtgtatgaa aaagaattta aatcaatttt
      361 aaggtgtgtt gagtaatgtt cttgatagtt tatatgttga tgaatttcaa aaaaataagc
      421 actcttataa aaagagtact tttggtatat attcaagcat ttatctaata caattatagc
      481 atgtcttatg ttttagtatg ataattttcg ttcgttttat tattacaggt tatactatag
      541 ttttatgtat cctgttaata ttttttacta ttttttcaat gttttgttta agtataaaaa
      601 gagataacta ttttaattct agttacctct ttttgtgtta ctttggccat atagatatac
      661 ctattgtaat agccattgta gcaattccta ttatagtagt tatacataat gcaataatcc
      721 atttattagt tgaatcaatt ctatcttcta taccttttat actattcttt atttcactta
      781 catccttttc tgtcaccttt ttatgttcgt ttatagcttc ttttaaatcg ctttttaact
      841 caattctttc tctagctaaa tcatttttta gttctattct atctttgtct aaatcattct
      901 tgtattcttt aaaaataccc tttatgtcat taaattgctt atctatactt tcttttactt
      961 ccttattatt ttgatgtact cttttttcag tctctaatat tcttttttca gtttctagta
     1021 ttcttttttc ttcttcattc atattaccta cctcctctga agtagtagct aaaaaccaat
     1081 tatctccaat acatttattt ttctccatca aagtactatt taaagtatta taatagtctg
     1141 tatatttgtt gattttactt ttatccttgt tggaattaaa ctttattact ttttcttttt
     1201 ctttattata ttcacccttc ataactattc ttcctctatc atagactcta aatgttcaat
     1261 tattgattgt atttctgatt tagatagttc taattccaaa gattttttat catttctaaa
     1321 tagtttcaaa ataaaatcat tttctctagc caaagaagat atagaatata agccaattgg
     1381 attgattctc tgaatatatg agttatctaa tatatcatat gtactatttg aaaaagtata
     1441 caaatcattt ttaaagtttt ggcttatatt tacattttca ccttcattat aaaagtcttt
     1501 cagtttaatt ccatactcac ctataaattc tgggtcttct cccaaaattt catttgcttt
     1561 agctttaaat tcatgaatac aatgtggcca agttggcatc tgcatatata aattaagtaa
     1621 tagtattgtc ttttcaaata ttttgttatc cttttcgtat ttttttatac agttaggaaa
     1681 atacgctacc aaattagtcc cattttcata agccatctta actcccccta aaataatatt
     1741 atttaatact attctacatt ataattactt tcattgcact attatttttt at
//
LOCUS       DAYAOX010000355         1792 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.360, whole genome shotgun sequence.
ACCESSION   DAYAOX010000355 DAYAOX010000000
VERSION     DAYAOX010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.360"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            61..630
                     /gene="mocA"
                     /locus_tag="ACGI57_003639"
     CDS             61..630
                     /gene="mocA"
                     /locus_tag="ACGI57_003639"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFG9718683.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            627..1646
                     /locus_tag="ACGI57_003640"
     CDS             627..1646
                     /locus_tag="ACGI57_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HFG9718684.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT          717 a          164 c          345 g          566 t
ORIGIN      
        1 tagagatatt ataaaaatca tagaactatc aatatattat aaataccaag gatgatatat
       61 atgataaatg cagtgataat ggcatcaggc tttgccagaa gaatgggagc aaataaactt
      121 ttattaaatt ataaaggctg ttctataata gaacatgtat ttagagaagt tagcaagatt
      181 gattttcatc aagttgtagt agtaagtcaa tatgaagagg ttttgttatt gtgtaataaa
      241 tatggttttg aatatataga taataaaaat gcaaatatag gtcaaagtga atctataaaa
      301 ttaggagttt taaatagctc aaaatgtgat ggttatatgt tctttgtagg agaccagcca
      361 tttattgata acttatatat agataaaatt ataaatactt ttaaattaga tagagatttt
      421 atagtaattc caagatacaa agatatgtgt ggaaatccag tcatattccc atatagcaag
      481 aaagaagaat tgttggcact taaagaagat gaaaaaggta aaaccatttt aaaaaattca
      541 tcaaaaataa agtatgtaga agttcctaaa gaaatgttat ttgatatcga tacaaaaatg
      601 gactatgaga gaattggggg taaattatga agctaataaa aactatagat gctgttggac
      661 aagttttatg tcacgacata actcaaataa tacctggaga atttaaagga agaaaattta
      721 aaaagggtca catagtaaaa gaagaagata ttccagtgtt attatctctt ggtaaagata
      781 atttgtatgt atgggaaaag tcagaaggaa tggttcatga aaatgaaggc gcattgtttt
      841 taaaagaact aactgctgga gaaaatttag attttagtga aataaaagaa gggaaaatag
      901 attttatagc agcatgtgat ggtctattaa agatagatgt tgatgcactt tttgatttga
      961 attgtgttgg agaaataatg atggctactc ttcataataa ttttatagta aataaaggtt
     1021 taaaagtagc tggaacaaga gtgatacctt tagttataga tgagaaaaaa ttagaagatg
     1081 caaaaaaagt agttggtaat agaaaaatag taaatgtagt accttttaaa cctaagaaag
     1141 taggaattgt tactacagga aatgaagtgt tttatagcag aattgttgat aaatttggac
     1201 cagtaataga agaaaaagta aaaggctttg gttgtgaagt tataggacag actatatgcc
     1261 cagatgataa agaaattata aagagtgcaa taaaagaatt tataagtcaa ggagcagaac
     1321 ttatctgttg tactggtggg atgagtgtag acccagatga tgttacgcca acagctataa
     1381 aagaaactgg agctgattta gtgacatatg gttcaccaat attaccagga gctatgtttt
     1441 tacttgctta ttacggagaa gttccaatta tgggtatacc aggatgcgca atgtatcaca
     1501 aaactacagt atttgatata gttttatcta gggttttaat tgatgaaaag cttgataagt
     1561 atgatatagc tagatatggt catggtggcc tttgtatgaa ctgtgatgta tgtacttatc
     1621 ctgcatgtaa ttttgctaaa atataaagtt attaaaaaag agtatttaca aattaagtac
     1681 tctttttaaa tatgaaacaa aacagctaga atgatgtttt aaaaaaagca tatttattta
     1741 gaaattataa taacttattt tcaatgtgta aaagaatgaa taaagttgca at
//
LOCUS       DAYAOX010000356         1786 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.361, whole genome shotgun sequence.
ACCESSION   DAYAOX010000356 DAYAOX010000000
VERSION     DAYAOX010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.361"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(131..1522)
                     /locus_tag="ACGI57_003641"
     CDS             complement(131..1522)
                     /locus_tag="ACGI57_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFG9718685.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
BASE COUNT          637 a          320 c          189 g          640 t
ORIGIN      
        1 ttttaattat aatatctatt tgtgtaaagc tttttttcat aatcaaagag gaaattacat
       61 ttaattttat atttaaatgt aacttccttt atttaaagta attcatttat ttacaataat
      121 tcgtcatgaa ttatttttct tttgttagta aatccatagc cattaatctt gttaatacat
      181 ttcttattcc caataatatt aaaccaaata ttattaaaac tgttgctatt cctccaaata
      241 tctgtaaaaa accaagtgtg gctgtaaaac cagcaatctt tcctgataat aaacttgcaa
      301 aaaaacttgt tagataccag gctcccatag acaacgtagc atacttagct ggagctaatt
      361 tattgaacat tgcaagtcct ataggagata agaataattc acctactgtt gtaaatatat
      421 aagtcaatac tataaacaat atactagcct ttaccattcc atctggacca ttagctaatg
      481 tagcaaatac cataactata aacccaacac ccattattat cattcctaat cccattttaa
      541 ctggaataga gaaatcacct cttttggtat gagataattt aacccatagg gtagctaata
      601 ctgggcataa agccacacat aatatagcat ttagtgatgt aagccatgct actggcattt
      661 caaatccaaa tagagttcta tttacattat cccttacata aatcataaaa gaactttgtg
      721 tttgatagta tgctgtccag aataagacca caaataaaaa taaaataacc attgctatta
      781 tcctattttt ctccaactta gtaagtggtc catactcaat tttttcacct ttattacctt
      841 tagtcacagg atattttccg acttcattta ataattttgg tgcaagaagt gtaaatataa
      901 tcatacctaa catcatgcct ataccacaaa ttaaaaatgc catcttatat ccataagata
      961 ctatatctcc acttgctcct cttactgcaa atacattatc agaaagcaat ccacatatta
     1021 ttggagctat aaatgctcct acatttactg ccatatagaa taaactgtat gctgcatctt
     1081 ttcttgacac atcagtttta ggatatatat ccccaactat atttgagcaa cttgatttaa
     1141 aaaaaccatt tcctaaaata attattgata gacctaccca aaccattgca atagatgtag
     1201 atgaagcaaa tagtacgaaa tctcctattg ctattaaaag tgcacctata gaaatacatt
     1261 tttgttgacc taaaacttta tctgctaacc agccaccgac taatggtgta agatatataa
     1321 gacctgtaaa taagccaaac atagatgttg cttcttgagt agaaagacct aaaccacctt
     1381 gagctacact tgctgtaaaa aataatacca ataaagctgt taaaccataa taactaaatc
     1441 tttcccacaa aattgtcatg caaataagcc aaaaactctt cggatgacca ctttttttag
     1501 atacgctttc tgtacttgac ataaaaaaac gccccctttt gttttatagt taaatttaat
     1561 attaatattg ctttaaccat tttagcacat acttataata tcatcttttc tatgtttact
     1621 caatagatta tagcctgtat tttcattttt ttattattat ttttaaaatt atgaaaaata
     1681 ttattttttt agtatcttca gcagaaaatc ttaatatata tatatttaaa taacaactta
     1741 aatcttcaat tcttttttgt ttattttttc atttttgtaa attttt
//
LOCUS       DAYAOX010000357         1763 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.362, whole genome shotgun sequence.
ACCESSION   DAYAOX010000357 DAYAOX010000000
VERSION     DAYAOX010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.362"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..597
                     /locus_tag="ACGI57_003642"
     CDS             <1..597
                     /locus_tag="ACGI57_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718686.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            728..1750
                     /locus_tag="ACGI57_003643"
     CDS             728..1750
                     /locus_tag="ACGI57_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD0873 family tyrosine ABC transporter
                     substrate-binding lipoprotein"
                     /protein_id="HFG9718687.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
BASE COUNT          677 a          238 c          300 g          548 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
       61 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      121 tgcccttcta tgactgttag agaaaacttg tctttagctc ttaacaaagg aaaactatta
      181 aatctaagaa aatgtcttcg ttacaaaaga gatttcttag aaaatttatt ggaaggtgta
      241 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      301 caatcattat cactaattat gtcgtgctta acaaatccat cagttttatt acttgatgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
      421 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcat
      481 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
      541 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
      601 aatatatcta ttgtattaaa ctcggcttta aaataaagtt aaaattacaa taattatggt
      661 tactttgtaa taattcatat gaatatatga acatatataa caataaattt ttgaggggga
      721 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtat
      781 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
      841 ttctaaaggt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcatt
      901 agatgccact aggacaggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
      961 aaatattgat atagacttcc aaaatgctca aaatgacatg cctactacac aaagtattgc
     1021 gagtaaattt gcatctgata agaaggactt aatatttgct atatctactc catcagctca
     1081 ggcagcattt aatgctacta aagatatacc tatacttatt actgctgtga gtgaccctgt
     1141 tgctgctgga ttagttaaaa ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1201 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggcaaaaac
     1261 tataggagtt atgtacaata ctagtgaagt aaattccaaa gttcaagttg atgctttaaa
     1321 agaatatgca tctaaaaatg gatttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1381 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg tccctacaga
     1441 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     1501 tgttatagca gctgaatctg gtccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     1561 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggcgaatc
     1621 agtatctgat atgcctgtgg ctacatcaga tgatacagat atcattgtta atgaagatat
     1681 attaaaagct ttaggtatgg aaaaaccttc taatgaaaat atttcttatg taaagactaa
     1741 acaagaatag aaagatattt tct
//
LOCUS       DAYAOX010000358         1712 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.363, whole genome shotgun sequence.
ACCESSION   DAYAOX010000358 DAYAOX010000000
VERSION     DAYAOX010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.363"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            89..1687
                     /locus_tag="ACGI57_003644"
     CDS             89..1687
                     /locus_tag="ACGI57_003644"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HFG9718688.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
BASE COUNT          657 a          215 c          270 g          570 t
ORIGIN      
        1 ttttcttttt acaacaaata ctcactacct tttgatataa tatttttatc aattattatt
       61 atattcaagt tttgaaagga gtattaaaat gagtttatta gttttcggac ataaaaatcc
      121 agatacagat tcaatatgtt ctgcaatttc attagcacat ttaaagaaca aactaggaat
      181 aaaagcaaca gcttatgctt taggagatat cagaaaagaa gctaaatatg ccttggatta
      241 ctttaaggta gatgcacctg aaattttaga taatgttaga atacaagtta gagatttaaa
      301 ttatgataaa gtagtacctt taacaccaac ttcatctata ttagaggctt ataaccttat
      361 ggatgaaaaa aatgttaaaa cactacctgt aacttatgat gatggaactt ttgctggtat
      421 cattactatg aaagaaatag ctaaaaatct acttcatcaa cactttacaa ctatacacac
      481 atcactatca aacatttgta aaaaccttaa tggtactatt cttgtggact gtggtgaaga
      541 tataaaaggt agagtagtca ctttatcatt tggaatggag actctaatag agactttaaa
      601 agaaggagac atagctattg ttggagatag atatgatagt attgaatatg ctattgatac
      661 aaaagttaaa cttttaatac ttactaatca tacagagatt tcaaaagatt tgctagctct
      721 tgctaagata aatggagtgt ctgttgtttc tgttgaaagt gacaattata aaacttctaa
      781 tgtaataaat caatgtagtt tcttagactc tatagaagct acagaaaatt taattacatt
      841 caaagaagat gattatatgg atgaaataaa agagattatg ctagaaacaa acttcagatc
      901 ttatccaata ttagatgatg ataataagtt tttaggttta gtttcaaaag gtcatctatt
      961 aaacccatct aaaaagaatg ttgttttagt tgaccataac gaatatgctc aaagtgctga
     1021 tggaattgaa caagcaaaca tagttgaaat aattgaccat cataaacttg gtggtatttc
     1081 tactgatgtt cctatgtcat ttagagttat gcctgttgga tgcaatagta ctataattta
     1141 tcaaatgtac aaagaaaata atgttgagat accttatgaa atagccgggc ttcttttatc
     1201 tgctatatta tcagatacat tattatttaa gtctccaaca acaactgata tagataagaa
     1261 agcttgtgaa gaattaagta aaatagcaaa agtagacatg gaaaaatacg ctatggatat
     1321 gtttaaatgc ggtacttctt tagatgaata tactatagaa gaaatagtaa atatggactt
     1381 taaagaattt aatatgagtg gacatagagt tggtattgga caagtattta ccttagacat
     1441 agactctata tttgctaaaa aagatgagtt tttatcttat ataaattcga ctgattatga
     1501 caaactaatt cttgcggtta cagatataat caaagaaggt tcttatttaa tctataaagc
     1561 agaagacagc ttaattgcta acgcatttgg tgttgaaggt gttcaaggta catttgcacc
     1621 aggtgttgta tcaagaaaaa aacaattagt ccctaattta actactgcaa tcaaaaattt
     1681 taaataatat aaaattttta aataacgaaa ac
//
LOCUS       DAYAOX010000359         1695 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.364, whole genome shotgun sequence.
ACCESSION   DAYAOX010000359 DAYAOX010000000
VERSION     DAYAOX010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.364"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(105..1397)
                     /gene="eno"
                     /locus_tag="ACGI57_003645"
     CDS             complement(105..1397)
                     /gene="eno"
                     /locus_tag="ACGI57_003645"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFG9718689.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT          501 a          346 c          181 g          667 t
ORIGIN      
        1 aattgaaaaa tcaattaaca cttatctctt ttatatctta taagatgagc ttaaatatct
       61 tatattctct cttcattaat cttttttagt aaccatttta ataactattt ttttaagtta
      121 tagaaagatt ttaatccaca atatctagct tgctctccaa ccatttcttc tattcttaat
      181 aattggttgt acttagcaac tctatcagtt cttgatggag caccagtttt tatttgacca
      241 gcatttactg caacagctaa atctgctata gtagaatctt cagtttctcc tgatctgtga
      301 gatataacag cagtatatcc tgctctctta gccatttcta tagcatctaa agtttcagtt
      361 attgtaccta tttgattaac tttaactaat atagagttag ctactccatt ttcaattcct
      421 ttttctaatc tctctgtgtt agtaacaaat aagtcgtcac caactaattg taatttattt
      481 cctaattttt cagttaataa tttccatcca tcccaatctt cttcgtctaa accatcttct
      541 atagttatta ttgggaagtt gtttgaccaa tcttcatata aagcaaccat ttcagcagca
      601 gttaactctt ttccttctcc tgctaaaacg tattttttag tttctttgtt gtacatttct
      661 gtagcagcaa cgtcaagacc taacataacg tcttcacctg gcttatatcc agcttttgtt
      721 atagcttcaa ctattaattc taaagcttct ctgtttgaac ctaagtttgg agcaaatcca
      781 ccttcgtcac ctacaccaca agctaatcct ttttctccta aaactttctt taacgaatgg
      841 aatacttctg ctcccattct taaaccttct ttgaaacaac aagctcctac tggtaatatc
      901 ataaattctt gaacatcaac gttgttatca gcatgctctc caccgtttaa tatgttcatc
      961 attggaacag gtaattgttt agcatttact ccacctaggt attggaataa tggtaaacct
     1021 atttcatcag cagctgctct agctactgcc attgaaacac ctaatatagc attagctcct
     1081 aatttacctt tgtttggagt tccatctaat tctatcatga ttgcatctat agctggttgg
     1141 tcaaatgcat ccattccttc tatttcaggt gctattattt catttacgtt agcaacagct
     1201 gtttctacac cttttcctaa gtatcttccc ttatctccat ctcttaattc aacagcttca
     1261 aaagctcctg tagatgctcc tgatggaact attgctcttc ccattgctcc atcttctaat
     1321 actacttcaa cttctacagt tggatttcct cttgagtcta atacttctct agcatatact
     1381 aattcaataa ctgacatttg tatatctccc ttcgattttc tattagttaa attttcattt
     1441 actgtaaaca tccgtctact attttaatct actttctata ttatagtaca taaagttgct
     1501 tttgaaaatt aggtacaaat ttatttgatg ggtaaatttg taaaagttcc tatttttatt
     1561 ttatttttta taatatttta atttatataa tataaaaaaa cttgtctaaa atccaaaacc
     1621 aatcttaaat acacaaatat tataactaga tttaaaacat taaacataaa atgtacaact
     1681 aatgattcta aaatt
//
LOCUS       DAYAOX010000360         1678 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.365, whole genome shotgun sequence.
ACCESSION   DAYAOX010000360 DAYAOX010000000
VERSION     DAYAOX010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.365"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            85..1575
                     /locus_tag="ACGI57_003646"
     CDS             85..1575
                     /locus_tag="ACGI57_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9718690.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
BASE COUNT          635 a          178 c          350 g          515 t
ORIGIN      
        1 ataatatttt atgtatatat taataaaagg acatattgtg ttataatggt atcgtataaa
       61 atcatttacg ggggtggtat taaaatgttt caatttgaaa cacaaataca caaattaaga
      121 catgatgttt taactgcaat agctagactt ggaaaagaag ataaccttac cagagaagca
      181 atggataata ttcagtatga aataataaaa ggtgagaaac ctacatacag atgttgtgtt
      241 tataaagaaa gagctataat atctgagaga gtaaatgtaa gtatggggtt acaaccaggt
      301 caagataaga tagatttaga tacaatggat atagaagaag atgaacaaat tatatatatt
      361 ttggaagctg catgtgacag ttgtccaata aatgaattta cagtaacaga tatttgtaga
      421 ggatgtctag cacatagatg taaagaagct tgtaaatttg gagctataac acatgtcgga
      481 ggaatggcct atataaatca tgaattatgt aaggcttgtg gaatgtgtaa aaaagcttgt
      541 caatatgatg ccatctcaga ggttgttaga ccatgtaaga gtgtttgtcc aactaatgcc
      601 ttaggatttg atagagaaaa tatgaaagct atgatacatg aggataaatg tttaaactgt
      661 ggagcatgta tgtcagcatg tccatttgga gcaatatctg ataagagttt gatagctcct
      721 gtagctagaa agttagttca aaaagagaaa atgtatgcgg tggtagctcc agctataaca
      781 ggtcaagttg aagcaaatgt tacatatggt caaataaaaa atgctataaa atcactagga
      841 tttgttgatg tattagaagc agcttgtgga gcagatgcag ttacagttca tgaaagctta
      901 gaatttgctg aaagaatgga aaagggagaa gcttttatga caaattcttg ttgtccagga
      961 tttgttggtt atatagaaaa aacataccca aatcatgtag ggaaaatatc ttctacagta
     1021 tctcctatga tagctagtgg tagatttata aaaagtcatg aaccagatgc taaagttgta
     1081 ttcataggac catgtacagc caaaaagagt gaggcagcta agaaagaatt aaaaggagct
     1141 gttgattatg tacttacttt tgaagaaata atggcattat ttgaggcatt tgatatagac
     1201 ttggctaagt gttcaaatga agatatagat gatgcatcaa tttttggaag aggttttgga
     1261 gctgctggtg gtcttacaaa agctatagaa aattatcttg ctgaaaaagg tattggtgtt
     1321 aattttgaac ctgtaaagat ttctggttca agagaaatca agaaaactat gactatggct
     1381 aatttaggaa agctagaagg taattttata gaaggaatga tgtgtgaagg tggttgtata
     1441 aatggttctg gtaaattctt atcaggtgca aaggttaaag ccacttttga tagaaaaaat
     1501 tctcaatcaa ctaagaaaag tgtcttagca aatacaaaga taaaagattt tagtaatata
     1561 aatttagaaa aataaagata atacaaagca acaaaaatac atattgttaa tagagttgtt
     1621 ctaagttgag gtttaacttt tggacaactc tatttttatt tatatattta attattga
//
LOCUS       DAYAOX010000361         1647 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.366, whole genome shotgun sequence.
ACCESSION   DAYAOX010000361 DAYAOX010000000
VERSION     DAYAOX010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.366"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(60..719)
                     /gene="cprR"
                     /locus_tag="ACGI57_003647"
     CDS             complement(60..719)
                     /gene="cprR"
                     /locus_tag="ACGI57_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HFG9718691.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            1029..>1647
                     /locus_tag="ACGI57_003648"
     CDS             1029..>1647
                     /locus_tag="ACGI57_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HFG9718692.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKI"
BASE COUNT          603 a          248 c          156 g          640 t
ORIGIN      
        1 tattttacaa acattttgta gatatatata atattttcac taaacaacac ttatacttat
       61 tacacccatt tataccctat cccccataca gtttctattg tataaatatc atattgtttt
      121 aatttgcttc gtatattttt tatatgctct gtgatagtac ttatatcact ttctccatca
      181 aaaccatata ccgactcata tattctttct tttgaaaata cttgtccctt attttttgct
      241 agaaattcac atattagata ttcactttta gtaaaattaa tttttttacc atcaattgaa
      301 acttctttgc ctaaaaagtt aaattgaaca tttgagatag ttaataaatt ctttttttct
      361 ctactttctc gccttaaatg agcagttatc ctagccctta attctcctac accaaaaggt
      421 ttggttatat agtcatctgc acctatacca agaccgtaca taatatcact ttccatattt
      481 tttgctgtca aaaacagtat tggacaatca accttatttc ttatagtttt acatacttca
      541 aaaccatcca tcttaggcat cataacatct aatattatta gattaaaata attaaaatcc
      601 atgttcatag ctttttctac atcactaatt acctttactt catgtccatc tcttttcaac
      661 acattttcaa tcaactttaa catatctaac tcatcatcta ctactaatat cttagacata
      721 ataactcctc catttatccg tttaatagta ttctaattta tatatttaat aatatcttta
      781 aaattcacat taaccataat actctcatat tatagcttac aataattaat tactatttta
      841 caaataaaat atcttagata caaaaacttt atatttcaaa actttatatt cattttacac
      901 gtatttactt aaatataaaa atttatttaa caatttatta ttgtaatatt ttgaatatat
      961 aataaaatta ataataagaa aactttttat aaatatactt ttaattgttt aaattggagg
     1021 aaaaaattat ggatgaaaag ataacaaaaa ataattataa tgttccattt gactatgttc
     1081 gaaaaaaact caaagaatta gaccctcata caattagtaa tttaagtaat atacctttca
     1141 ataatgattt aaatagattc acactcaatt tttttgatag acaatatatt atagactatc
     1201 caagtggaga agtttggaat agtaatggta gcctttgcaa taactttgag cttaaaatct
     1261 tgatagttag atatttaatc aatgctaaag gtattcctag aactaataaa tatgtaactt
     1321 ttaaagaaat accaggaggc aatgtttatt atccaaattt tttaaacagg actctatata
     1381 ggctttcaga agtttttaca tctcaaatag acaaatataa gttagttatg gagaaaattg
     1441 gagctgaaaa agtaaacatg ggagatttag cttataggtt tacatatatg ggtaatacat
     1501 ctatggtatt tatactttgg tttggagatg atgaatttcc tccaaattca aatatattat
     1561 ttgattctaa tatagtttat tacttcaatg cagaagattt agcagttgtt cctgatacag
     1621 ctataaatgc aattttaaat aaaataa
//
LOCUS       DAYAOX010000362         1638 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.367, whole genome shotgun sequence.
ACCESSION   DAYAOX010000362 DAYAOX010000000
VERSION     DAYAOX010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.367"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(444..1151)
                     /locus_tag="ACGI57_003649"
     CDS             complement(444..1151)
                     /locus_tag="ACGI57_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFG9718693.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          531 a          277 c          142 g          688 t
ORIGIN      
        1 atagtataat aaatatagtt ctttaatctt ttgggtatat atacttaatc attttgaaat
       61 ttgatttaaa gtataataaa atatataaaa aaatatataa aatggtacta taaaattttt
      121 aatcttcatc aatacctttt ttgctttcat tgttaaaact attttttcta ttagtttatt
      181 tcttataaaa caaacattta gtattaaaat atatatcaca caatagtatt tgcaatttaa
      241 ctttaaaaaa ataaaaatac ccaactgacc taagcaactc gattgggtat aaataatcta
      301 aaaaatcatt tttgtataaa ctctttattt aacttacatt tttatttacc ttctacttta
      361 attcaactta tatttctatg cttcttatac tctcattcaa cttatatttc tacttatctt
      421 atacttttat aaattcatca caattattta gttctcgcta tcaaagaaat tgtattaaaa
      481 tactttccat tctctacttc catcattttt ttgtcattta tagcatatga attatcacaa
      541 tttatccaat ccttccaagc ttcatcatga caatttaaag cttcacattt ctctacaatc
      601 acagactccg attcagacca aagtttattc caccatgtaa ttgaatggaa gttcatatta
      661 tcttgccaaa atggaatgag ttcatctggt atacaatcca cgaaatcttc ttttaatcca
      721 ggcattgcca tagcaagtat tcccccctct tttacaagag gtgatatatg attgtctaaa
      781 aatccttctt tgtttccaaa atagtgataa gaatcgatac ttatcaccgc atcaaagaaa
      841 ccttctgcat aaggtagttc atgtgcctct gcctgtattg gaaatatttt atcatccaat
      901 ttaaactctt taaatctttt atagttttca gttggttcta tccataaatc tgttgcaaat
      961 acagtagcat caaattcctt tgctaaaaat atcgaagata tacccttacc acaacctaag
     1021 tctaaaatac gcattccctt ttctagtttt atcttacttg ttaattcttc caatattttt
     1081 atacaattag gtcccatcat atttttcatg agaaagtcct tatcgtattt attacttttt
     1141 gtaaatatca ttctatcttt cctttcccta tcagttatta tttattaaat tttatacgtt
     1201 tgatttttta ttttttatct gcttcttata ttgagcaatc tcttttttat tcattcttta
     1261 acagacaatt ttaatgtgta attttatcca ctctgtgata gtaactttaa tgaataatct
     1321 tatctactgt gatagttatt ttagtgagta attttatcta ctccacagca atcgctttaa
     1381 tgagtaatct tatccactct atgatagtta ttttagtgag taattttatc tactccacag
     1441 taatcgcttt aacgagtaat cttatctact ctataatagt cactttaatg agtaatcata
     1501 atttttatat cattttagat acttactcta ataagaattc attttctaat acttaataag
     1561 ttttaattac ttccacttta acctatatac taattaactt atttaattta gtgcatattt
     1621 gacactttac cttttatt
//
LOCUS       DAYAOX010000363         1635 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.368, whole genome shotgun sequence.
ACCESSION   DAYAOX010000363 DAYAOX010000000
VERSION     DAYAOX010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.368"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            296..1432
                     /locus_tag="ACGI57_003650"
     CDS             296..1432
                     /locus_tag="ACGI57_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFG9718694.1"
                     /translation="MIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLIN
                     EIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYMI
                     YNNHRALEYGLDNLDELYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEIIH
                     TGLEPLKIYDFISKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYLY
                     LIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEMR
                     NMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLKA
                     GIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
BASE COUNT          705 a          134 c          229 g          567 t
ORIGIN      
        1 atttaattta attaaaatat tataacatat tagttacata ttaaaatata taatcgagat
       61 attgttaaga cattgtcgag atattataat atataactaa tgtattataa ataatagttg
      121 taaacttcct gtaacatata ttttaaatgc ttattcttaa atttttgcat tatttattaa
      181 aatttattat gctttaacgc taaattgagt ttgataatta taaaacaaat aatcaaaaat
      241 agtgtaaaaa ttaatacttt ttgttaatat taatagataa agataatcta aggtggtgat
      301 taaactgaca atagaagaat acaaaaacaa atactttact ggaaataaaa tcgtactttc
      361 tataaatagt tttgataata aagaactata ctatcatgaa actgaaaata tattaaattt
      421 aataaatgaa atagataaat taacatcaag taagatattt aaaacaatag aagataaaaa
      481 tgatttggca ctagagtctt attattctag tgtaatagaa ggtgcttttt caactagaaa
      541 gattgcaaaa tctataatta gaggaaagat gaaaccaagt aataaaagtg aatatatgat
      601 ttacaataat catagagcct tagaatatgg attagataac ttagatgaat tgtatagtca
      661 caaatttata tatgatttgc atcatatatt gggcgaaaac tgcttggatt cagaagaata
      721 tgagtataga actgaaaaag tatatgtatg tgattcaaaa ggtgagatta tacatacagg
      781 gcttgaacct ttgaagatat atgactttat tagtaaatta atagatttta tggaaaatag
      841 caaagtaagt aatttaatta aaagtgctat aattcatttt tactttgtat atgttcatcc
      901 ttttagtgat ggtaatggaa gaacatcaag agccttatca tatctatatt tgattgataa
      961 aggatatgat actttcaagg atttttcaat atcatatatg atatctaaaa acagaactaa
     1021 gtattataag gctatacttg atgtagaaaa caaaggaaat aatttaactg tatttataga
     1081 atttatgctc aagtctataa tacaatctat taatgagatg cgtaatatgc atgatagaaa
     1141 atctttagaa agtatattaa aagaagaatt gtttgaaaat gacataactc tatcagcaac
     1201 agaagaacat atattaaaat atatttgcaa taaagataac tattctatga cattagaaaa
     1261 ttatataaaa aagaataaaa gtagatactt aaaggctgga ataaaggaaa ttgaattagt
     1321 tgaccagttg atggaagtgt ttaataatct tgaagagata gagattttgt ctaaagaaaa
     1381 aaatatatat aaagttaatg ataagtactt aaaaatgctt gatatagact aatttagata
     1441 atctggagag ggaatatatg aataataaga aaaaggcatt tttgatttct ggggctataa
     1501 acgtaatttc attaattatt gttttttcat tgtttcatta taatattata gaaaatagta
     1561 cacaaaatat gataataact gtaatcttaa tttttatttt tgaaatgata aaaataaaaa
     1621 tcatagagaa atact
//
LOCUS       DAYAOX010000364         1618 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.369, whole genome shotgun sequence.
ACCESSION   DAYAOX010000364 DAYAOX010000000
VERSION     DAYAOX010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.369"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            79..1584
                     /locus_tag="ACGI57_003651"
     CDS             79..1584
                     /locus_tag="ACGI57_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9718695.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLINLNKELDTLEKQKE
                     RLHDFLEQGIYDSNTFGERLKILLSRIENAREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
BASE COUNT          703 a          167 c          270 g          478 t
ORIGIN      
        1 tattttgaag gtttattttt gtgttttaaa tataatcaat ctgttactga atataatata
       61 ttcaaggggt gtgatttaat gaatgtggca atttatttac gcaaaagcag agctgatgaa
      121 gaagcagaac atagaggtga atttgaaact ctaagtagac ataagacaac tcttttaaaa
      181 ctagcaagag aacaaaattt aaatgtcatt gaaattaaag aagaacttgt ttctggggaa
      241 agtatagaat atagacctaa gatgctagaa ttactaggtg aaattaaaaa taataactat
      301 gatgctgttt tagtgatgga tattgaccgt ttgggtcgtg gtaatatgca agaccaaggg
      361 ttaatactag atacatttaa aaaatctaaa acgaaaatta ttactcctag aaaaacgtat
      421 gatttaaatg atgagtttga tgaggagtat tctgaatttg aagcttttat ggctcgtaag
      481 gaattaaaat taatatctag acgtatgcaa agaggaaaaa tcaaaagtat tgaagagggt
      541 aaatttatag ctcaagctcc tccttatggg tatgatatta aatatttaaa taataaagaa
      601 agagtacttg taataaatga agataaagca actgttgtta aaataatctt tgatatgtat
      661 ttaaaaggag atggtgcatg taaaatatcc aattatttaa acgaacttgg ttataagacc
      721 acaacaggta gaacttggta tgaaaagggt ataagagata ttataaaaaa caaagtgtat
      781 tgtggatata tagtatggaa taaagtcgag aggaaaagaa attctagtag aacaagagga
      841 attgaggaac aaattgaggt aaaaggaaag catgaaccaa taataagtga agacatatgg
      901 aataaggcac aaaatataag aaaaaaacgt attaattccc ctactccaaa caataaaata
      961 acaaatccat tggctggaat tgtaaaatgt ggatactgta atcatacttt aatagccaaa
     1021 tctaatacta catcaaatgg atatgagaaa tatatggttt gtactaattg taaaaataaa
     1081 gctactaaac tatacatatt agaaaataaa atattagagg tcttaaataa atggttagta
     1141 aattatagac tatctttaaa agatgaaaaa gacaactcta aacctgaaaa tactttagaa
     1201 cataccttga taaatttaaa taaagaattg gatactttag aaaaacaaaa agaacgacta
     1261 catgattttt tagaacaagg aatttatgac agtaatacat ttggggaacg tttaaaaata
     1321 ctcttatctc gtatagaaaa tgcaagagaa gctattaata aaacaaatat agatattgaa
     1381 atgcaaaagg aaagaaatac agttaaaaca caaattgttc ctcaacttga aaaaatttta
     1441 agtgaatata cagaggaaaa agaaccttat ctaaaaaata tattgcttaa aagtgtaata
     1501 aattacgcag tctataaaaa agaacctaat caaaaatctg atgatttttc tttaatactt
     1561 taccctaata tcccaagaga ttaattatat gttttaaatg ttataaatag actgatta
//
LOCUS       DAYAOX010000365         1584 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.370, whole genome shotgun sequence.
ACCESSION   DAYAOX010000365 DAYAOX010000000
VERSION     DAYAOX010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.370"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(110..1021)
                     /locus_tag="ACGI57_003652"
     CDS             complement(110..1021)
                     /locus_tag="ACGI57_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9718696.1"
                     /translation="MAVISFCILHLITVSTISLVKNTSPDIIFANMTYKLISILIASI
                     LDFILWYSMNKIKEHIYIITQNQIEFSLFLKFIWFTLGYILFDSIQITFAPPSSLTSY
                     FILGSNILLILQLIIFINHIYSIEKNIYLEKEHNRLKMIKQEQLLKVNLLKKKANIDE
                     LTSAYNRKFAFEKIQHYIDNNLLFSLIYIDLDKLKTINDTYGHLAGDNYIINFSSIVI
                     ENLGIDDIFARIGGDEFIVIMPKSNENTTKEFIEKISKIMDNTISFSYGIVEVSSENK
                     MTVEEIINIADIRMYTNKQNNHKRNNN"
BASE COUNT          566 a          158 c          198 g          662 t
ORIGIN      
        1 tttagagtta ttaatcatta tgtccagata tttaaaaaaa ttataatgat agagataata
       61 agataaaaaa catagtgaga ctacaagtaa gctcatgtaa atattcattt tagttattgt
      121 tccttttatg attattttgt ttattagtgt acattctaat atcagcaata ttaataattt
      181 cttctacagt cattttgttt tcgctagaaa cttctacaat accatagcta aaagatatcg
      241 tattatccat aattttactt attttctcta taaattcttt tgtggtgttt tcattagatt
      301 taggcataat aactataaac tcatctcctc ctattctagc aaatatgtca tctattccta
      361 agttttcaat aactatactt gagaagttaa taatatagtt atccccagct aaatgtccat
      421 atgtatcatt tatcgtcttt aatttatcca aatcgatata tatcaatgaa aataataaat
      481 tgttatcaat gtaatgctgt attttttcaa aagcaaattt tcgattatat gctgatgtta
      541 attcatcaat gttagctttt ttctttaata aatttacttt tagtaattgt tcttgtttta
      601 tcatttttag tctattatgt tctttttcta aataaatatt tttttcaatg gaatatatat
      661 gattgatgaa tataattagt tgtaagatta ataatatgtt acttcccaat ataaagtagg
      721 aagttagaga acttggtgga gcaaatgtaa tttgtatact atcaaataat atatatccta
      781 aagtgaacca gataaatttt aaaaatagac tgaactctat ttgattttga gttattatat
      841 aaatatgctc ttttatttta ttcatagagt accataagat aaaatctaaa atagaagcta
      901 ttaaaatact aattaactta taggtcatat ttgcaaatat tatatctgga cttgtatttt
      961 taacaagtga tattgtactt acagtaataa ggtgtaaaat acaaaaactt attactgcca
     1021 tattgggaat aaaatggttg ttagtagaac tttttctgct taagagcaat ttatgactta
     1081 ctatgtaaaa caatatataa tatagtggta atggtaattt tgtgaatatt attatcaaga
     1141 aggttaatgg ttttattaaa aaaatatatt tgtaattctt atgtatagtt atatttttgt
     1201 ttccaaaaga tgctattttg catatgtaaa tgttacatag ttcgattatt aatatagtag
     1261 ttatgattaa aaatatatgg tatttcatag tacatcctct tttaaagtga aattgtattt
     1321 acttaaaata gattagattt tatatattct attttacagt ttttatttag gtacatgcaa
     1381 agagcttatt atggatacct tcttttttat tgcatgtgtc tgtgttatat aaaatttaaa
     1441 aagtattata tcttaatata cagtatgtat gataactatg tgtccaaata tagtaattat
     1501 gtatggaaag tataatagtt atgtgtataa gtatagtaat tacatatagc atgtatgata
     1561 gttatgtgta taagtatagt aatt
//
LOCUS       DAYAOX010000366         1565 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.371, whole genome shotgun sequence.
ACCESSION   DAYAOX010000366 DAYAOX010000000
VERSION     DAYAOX010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.371"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            4..402
                     /gene="tnpA"
                     /locus_tag="ACGI57_003653"
     CDS             4..402
                     /gene="tnpA"
                     /locus_tag="ACGI57_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFG9718697.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            399..1562
                     /locus_tag="ACGI57_003654"
     CDS             399..1562
                     /locus_tag="ACGI57_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFG9718698.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNKGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
BASE COUNT          672 a          166 c          277 g          450 t
ORIGIN      
        1 tttatgtata aatctaacaa aaatatagtt tatgattgca aatatcatat tgtattttgc
       61 cctaagtatc gtaaacctgt tttagttggc gaagttgaaa agatgttaaa agaaatacta
      121 ccatataaag cagatgaact tggagcagaa ataatagaaa tggaaactga taaagaacat
      181 gtgcacttat taatatcttg tgaccctcaa tatggaattc ataaagtagt aaaaggttta
      241 aaaggcttta gttcgagagt attaagagag aactttccac atttaaagtc tagtatgcct
      301 agcatgtgga ctaatagcta ttttgtaggt actgttggtt ctgtgagttt agaagtagtt
      361 aaacaatata tagaaaatca acaagttagg acaaagaaat gataaaaact catagagtga
      421 aattaaacct aactagaaca caatttgaat tagtaaggga aaagcaaatg gaaagtgcta
      481 actgttggaa ctacatagtt aacttatcta aagaatacta ttttgaacat aaacaatgga
      541 ttaaaaagaa tgatatacaa aagttaatca aaggtaagta caatcttcat agtcaaacta
      601 ttcaagctat atcagataaa tttgatgcaa atagaaagac tatatcagaa ttaagaaaaa
      661 aaggtaatac taaagcaaaa tatccataca agactaagaa attttatata atacctttta
      721 aagctagtgc tattaataga aatgctaaag gcaacttaaa attatcaatg tcaaaaggca
      781 ggtatttaga attagatttt aatgttgaga atataaaaac tgctgaaata gtatggcgaa
      841 atggatatta tttatactac acttttgaca atgaattaaa tggagttgta gccaaaggca
      901 ctaataccgt aggtgtagac ttaggggaaa tacattcgat agctagtgta acaaataaag
      961 gtgtagggct tattctatct aatagagaag gcagaagtat taaacaattt agaaataaga
     1021 tgtatgctta tatatctaaa agattaagta aatgtaaaaa aggtagtaga caatcaaaga
     1081 agttatggag attaaaaaat aagataagaa gtaaaacaga taatcagtta atgaatttat
     1141 atcatcaaac aactagaaag tttatagact tttgtgtaga gcagaaggta tgtgaaatag
     1201 ttcttgggga tataaaaggg gttgaaaaag atactaagaa aaagaaaaga ttaaacagag
     1261 ttaatagaca gaaaatatct caaatggagt atggtcgaat aaaggactat ataaaatata
     1321 aagcaaaaga gcaaggtatt gaagttaagt tagttaaaga aaactacact tctcaaactt
     1381 gcccaaagtg ttctaaaaaa cacaaaccaa ctggtagaac ttatagttgt acttgtggtt
     1441 atgaaactca tagagatata gttggtgctt ggaatatttt aaataaaaag cataaatatg
     1501 gtttagtaga ttttagaata aatcataagc aaccaataaa tttaaaagta tctaccgttt
     1561 gagcc
//
LOCUS       DAYAOX010000367         1548 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.372, whole genome shotgun sequence.
ACCESSION   DAYAOX010000367 DAYAOX010000000
VERSION     DAYAOX010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.372"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(491..>1548)
                     /locus_tag="ACGI57_003655"
     CDS             complement(491..>1548)
                     /locus_tag="ACGI57_003655"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9718699.1"
                     /translation="ILTDKNSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNDI
                     TKDNVTNNRVYGADRHETNANVIKKFYTDDELEAVLVAKSDVLVDALAAGPLAANLKS
                     PILITPKTYVSAYHKENLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNS
                     GGKTVMIDPGHGGSDTGTTGKPLGGIREKDYTLNTSLATTEYLRSKGFNVIMTRDTDK
                     TLSLGNRTALSNSLRPDLFTSIHYNASDTTGNGVEVFYKLKDKDGGTTKTVATNILNR
                     ILEKFNLKNRGAKTRTLSTDPTKDYLYVLRNNDMPAVLVECAFLDNEKDMSLLNTSNK
                     VKEMGTQIGKGIEDSLK"
BASE COUNT          498 a          256 c          171 g          623 t
ORIGIN      
        1 ttaacataaa tttctacttt atttttaatc ttttaacttt tgaaaatatg tcaaagtgta
       61 tctaacccaa aatataaata atatataagt aatatataaa aataaactta taaaactcaa
      121 aaaataaaaa aattaaatat atttaataca tttaattatc tatactatat catttattga
      181 cataaaaatc cacaaaaaac acacaaacac acaataaaat atagtaatta agcttttatt
      241 gtgtattaca aatgaatact ccaactcttg acaattaact gtagccttaa gatatgtaaa
      301 ttcttcatat aatataaatc cttttacaat taatcataaa aagactgtat atacctcatt
      361 tcgcacttat atgcacaatg tatatacagt ctttattact attataatta cattcttatc
      421 ttatagcttt agtttaaatt taaaattgca gattaattta ttttaaattt ttctatgctc
      481 tcattgtaga ctattttaat gaatcttcta tgcctttacc tatttgtgta cccatttctt
      541 ttactttatt tgatgtatta agtaaactca tatctttctc attatccaaa aatgcacatt
      601 ctacaagtac agctggcata tcattatttc ttaaaacata taaataatct tttgtagggt
      661 cagttgatag tgttcttgtt tttgcacctc tattttttag attaaatttc tctaagattc
      721 tatttaatat atttgtagct acagttttag tagtccctcc atctttatct ttaagcttat
      781 aaaatacttc tacaccattc ccagttgtat cagatgcatt atagtgtata ctcgtaaata
      841 aatctggtct caaagagtta gataaagcag ttctatttcc aagagataaa gtcttatctg
      901 tgtctcttgt cattattaca ttgaatccct ttgaacgtaa atattcagtt gtcgcaagtg
      961 aagtatttaa tgtatagtct ttttctctaa taccccctaa tggtttacct gttgttccag
     1021 tgtccgaacc accatgtcct gggtcaatca taactgtctt gcccccacta tttcctggtt
     1081 ctgttggagt tgtattgtgt ttagataatg atgatgctat agagctcatt accttagaag
     1141 tcaatcctcc acctatttta tataccttat ttgccgattt agcttctaag ttttctttat
     1201 ggtatgcaga tacatatgtc tttggtgtta taagtattgg agattttaag ttcgcagcca
     1261 atggacctgc tgctaaagca tcaacaagta catctgattt agctactaaa acagcctcta
     1321 actcatcatc tgtatagaat ttttttatta cgtttgcatt tgtttcgtgt ctatctgctc
     1381 catatactct attattagta acattatctt tagttatatc atttacctta tttataacat
     1441 ttgttgatat catttgaggg ccacctataa aataagcatt ttgtaaatcc tcactcttta
     1501 accatttata tgtgttgtct gtaatactat ttttatcagt taatataa
//
LOCUS       DAYAOX010000368         1534 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.373, whole genome shotgun sequence.
ACCESSION   DAYAOX010000368 DAYAOX010000000
VERSION     DAYAOX010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.373"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            351..917
                     /locus_tag="ACGI57_003656"
     CDS             351..917
                     /locus_tag="ACGI57_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9718700.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQINNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            957..>1534
                     /locus_tag="ACGI57_003657"
     CDS             957..>1534
                     /locus_tag="ACGI57_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9718701.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
BASE COUNT          626 a          149 c          262 g          497 t
ORIGIN      
        1 ttgttgtttt taagataaaa attaaatgta tcaattattg ttttatacaa gtatttccaa
       61 atccgttctc atttagatag tatgcttaga aattttagaa ttattgagtc taactttatt
      121 acttacttta ctttataaca atcaaataca aatttaaata ttataaaact acaaaataat
      181 ttaaagcata taatgtcaat atgtttaaat tataaaatat tcatgtagtt atataataaa
      241 taatttataa atatagccaa cttagcctaa atacgtatat aatatagcta atatggttct
      301 tatacttgat accatcaata aaactaaaaa tataaaaagg agaacttaaa atgagtgtta
      361 gtttacaaaa aggacaaaga gttagcttag taaaagataa taatcctgta aaaaatctag
      421 tagtaggctt aggatgggat atgaataaat tgggtaaaaa aaattatgac ctagatgcat
      481 ttgcaatagc attgactaac caagataaaa tgaatgttag aggtgacttg gtttattttg
      541 gaaatcttaa acacccttct aaagctatta tacacactgg agataactta actggaaaag
      601 gtgaaggtga tgatgaacaa ataattgtta atttagaaga catacctgaa tatgtacata
      661 aaatagtttt tggagtttct atatacaaag ctaaaaaaag agaccaggat tttggtcaaa
      721 taaacaactc ttttataaga ttaattgatt caaattccaa gcaagaatta tttaaatata
      781 atcttcaaga agacttttct ggtaaagtaa cagttttggc aggagaaatt tatcgtagaa
      841 atgaagaatg gaaatttaat gctttaggaa ttggtcaaaa tgaagagctt cgtgaattaa
      901 ttaatacata taagtaataa ttacaataaa aaatttataa tgaggagttg gtttaaatgg
      961 gtataacttt agcaaaagga caaaaagtaa gtttaacaaa aagtaaccct ggtctaaaaa
     1021 aagtaatagt aggactagga tgggatatta ataaatatga tggtggcttt gatttcgatt
     1081 tagatgcctc tgcattttta actggaactg atggaaaagt aacgaatgat ggagacttta
     1141 tattctataa taatttaaaa cacgcttctg gagctgttga atatatgggt gataatagaa
     1201 caggtgttgg tgatggtgat gatgaacaaa taaatgtaga cttatctaaa atacctcaaa
     1261 atattgctaa aatatcattt agtgttacta taaatgaagc aattacaaga agacaaaatt
     1321 tcggacaagt tgaaaattca tatataagaa tatataatga agaaactaat gaggaactta
     1381 ttaaatatga attgggtgaa gattttagca tagaaacagc aatagttgtg gcagaactat
     1441 atagacataa tggagagtgg aaatttaatg ctttaggttc tggatttgag gatggacttg
     1501 caggattgtg taaaaatttt ggcgttaata tagg
//
LOCUS       DAYAOX010000369         1532 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.374, whole genome shotgun sequence.
ACCESSION   DAYAOX010000369 DAYAOX010000000
VERSION     DAYAOX010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.374"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            37..1473
                     /locus_tag="ACGI57_003658"
     CDS             37..1473
                     /locus_tag="ACGI57_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HFG9718702.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          625 a          160 c          268 g          479 t
ORIGIN      
        1 atattaaaaa tttattatga gggggacaat tttattatga aattctataa aagaatatta
       61 acattgactt taagtatttc ttttgtattt gcaaatattc aattagtaga tgcaatatca
      121 tctgtagaaa aaatacaagg aaaaaataag tatgaaatcg caggtaaaat agcagataaa
      181 aatgcatata aaactgctat attaattaat actagtaact ctattgcaga tggattaagt
      241 gcaagtggac tagcaggagc tttaaatgcc cctatactat taactgaaaa aaatactata
      301 ccaactgaga catctgcaag attaaaaaat gtatcaaaag tttatataat tggaggaaca
      361 tattctatta gtacatctgt cgaaaattca ttaaaatcta agaaaatgaa agttgtaaga
      421 attaagggaa atgacagaat aaaaacaagt tataatgttg caaaagaaat aaattcaata
      481 aaaaaagtca atactgttat gctaaccaat gcatataaag gagaagcaga tgctataagt
      541 attgcatctg tagcagcaag agataaagca ccaataattc ttactaatgg acaaagtata
      601 cctttttcaa ctagtggttt aaagtcttat gtaataggag gaacagcttc aatgagtacc
      661 actttagtaa acaatactaa atctactaga ctagggggga gtactcgttt tgaaactaat
      721 aaagcaataa taaataagtt ttataaggat gctagagagt tttatatagc tggagcttat
      781 gaattaacaa atgcacttgt aggatcttct ttatcaaaac atggtccaat ggttttagtt
      841 aatgatggta gcaataaaag cattttaaaa aatgctaaga agataacttc tataggatat
      901 atagattcaa atatagtaca acagtgttta aatataacaa atggaattgg agatattaat
      961 actggggttg taaaaaatgt taaacctact acaaagacta taaaagatgg aatgtataag
     1021 gttggaaaag atatctcagc aggtgaatac ttaataactt caaactcagg aagttatgct
     1081 tcttattatg aagttacaag tgatagtaca ggtaatgctg atagtatact ttcaaatgat
     1141 atttttagtg ggactagata tatcacctta aagaatggtc aatatattaa aattgaggat
     1201 tcaacaatga cattagcaaa gtatgctaaa gcccagaagg ccaagaatgg taagtttgga
     1261 aatggtatgt ataaaattgg attagaaatt cctgctggag aatatattat aatgtcaaat
     1321 tcttctgatg catattatga agttagaaat gatagcttag gtaatgcaga gggaatagtt
     1381 actaatgata ctttttcagg aagaagatat ataacggttg aggaaggtca atatttaata
     1441 ttaaatgatt gttatttaat tgaaaatgaa tagatgtaat tgtatttaat agtaaatcat
     1501 atattttatt taaggaaagg tgttttaagt tt
//
LOCUS       DAYAOX010000370         1529 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.375, whole genome shotgun sequence.
ACCESSION   DAYAOX010000370 DAYAOX010000000
VERSION     DAYAOX010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.375"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..492
                     /locus_tag="ACGI57_003659"
     CDS             <1..492
                     /locus_tag="ACGI57_003659"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9718703.1"
                     /translation="IKVLFIMFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEG
                     ITTFHQFIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHG
                     VAKKLMTTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMK
                     LLI"
     gene            complement(861..1418)
                     /locus_tag="ACGI57_003660"
     CDS             complement(861..1418)
                     /locus_tag="ACGI57_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9718704.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
BASE COUNT          548 a          210 c          168 g          603 t
ORIGIN      
        1 ataaaggtgt tgtttataat gttcaaagaa tacttaataa gagaattaaa agaagatgat
       61 attaaaaatg cattagactt aatctggaac gtatttgaag aatttgaatc tccagattat
      121 tgtgatgaag gaattacgac atttcatcaa tttatagaat ataactctat tattgaaaaa
      181 atcaaagaaa aaactctctt cttttggggt tgttttgact ctaaaaaact tattggagtc
      241 ttagctatta gaaatttaaa tcatatatgt atgttatttg tagataaaaa ctatcataaa
      301 catggtgttg caaaaaaatt aatgactaca ggcatacaat actgtatttg taataaattt
      361 aatttaactg aaataactgt aaattcatca ccttatgctg ttgaagttta tcataaatta
      421 ggatttaaag atactgctgt tgagcaaatt gttgacggta tacgttttac acctatgaaa
      481 ttgttaatat aataaattaa ataaaaagca aagtctctta aatattttat agttactttg
      541 cttttaaacc aagatttaaa ataatactat cttttttgtt ttttaataat atactttaaa
      601 atccttacta atatgaacta taatttattt tttgatatcc tttttgatta acatataaat
      661 taattcaatt attaaacata aactatgttg tttctcttta tatctcttct ttgtaaacaa
      721 tattgggcat ttgaaaataa atattcttaa aatatactgt tctcctgttg attttccttt
      781 ggtacaaatg ataaaaattt aatttacatg ccccataatg aagtgttcaa taaataagtt
      841 aaaataattt atttacctcc ttaatcatat tttttatgaa tacctaataa cacagtatca
      901 atagaatcgg ctaaaccttt tatagcttct tctttactca aaagtgattt cccaagttgc
      961 aaagtcgaat aaccatgaag tacactcatt attaagttta atacttcacc tgtattcttt
     1021 gtatttagat tacaagaaag aataagtttt tctaataagg atttatattc atcaaagatt
     1081 tctaatgttt cattatttcc atgccaagta gcccactgaa ttgtttcata aacaccagga
     1141 ttagaaatta taaaattaaa gtatgctata cttatacact ttatagctgt atctccaaaa
     1201 tttccaatag tcgcctttat catttgacta ttcatctctc tcattccttt atgagctact
     1261 tcaagtaata aatcatctag actttctata tgattgtaca aagatggtgt acgtattcct
     1321 agcttttctg caataacttt taaagaaact ttgttaagcc catctttatt aactatatct
     1381 gatgtagctt gtataactat ttctctacta acacgcatga ttttccctct ttcatttaaa
     1441 attaattact ttattagttt agctaatcta attagtttcg tcaattattg taatttataa
     1501 aattctttat actattataa aatcttgac
//
LOCUS       DAYAOX010000371         1514 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.376, whole genome shotgun sequence.
ACCESSION   DAYAOX010000371 DAYAOX010000000
VERSION     DAYAOX010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.376"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(207..776)
                     /locus_tag="ACGI57_003661"
     CDS             complement(207..776)
                     /locus_tag="ACGI57_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFG9718705.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
     gene            complement(937..>1514)
                     /locus_tag="ACGI57_003662"
     CDS             complement(937..>1514)
                     /locus_tag="ACGI57_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9718706.1"
                     /translation="KILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAIS
                     FSSLVAAVSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIV
                     SSMLIFFTTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLL
                     SDRVYIIILGVGFITLLIAFVSKITLRNENL"
BASE COUNT          530 a          240 c          199 g          545 t
ORIGIN      
        1 ataatgacat ttgtatatta gaattgttta ttgattatta gaaactttta aggttagaaa
       61 gtttatgact attatacatt atattattta ttatatagtg agtttaaaat attaaacttt
      121 ttattctaca aaatataaaa agaagttgca tcaaaaagag aaatgctctt taaagatgta
      181 acttctatgg tatatgtatg gaggagctat attgccatct ctggaatcaa gcttctcata
      241 gcttctatag ttgtttggat aagcttatca agttcccatc caagcatttc tgcaccactt
      301 cttataacgt ctcgagaaca gccagcagca aatgatttat ctttaaattt cttcttaaca
      361 gattttaatt ctaaatcaga aacgctcttt gaaggacgca ttattgcagc agcaccaatt
      421 aaaccagtaa gttcatccac agcaaataat actttctcca taaattgctc tggtttaaca
      481 tctgaaccag ttaatccata tccatgactt attgtagaat cgattattga tttatctaaa
      541 tcaagctctg tcattaattc tctttgttta acacaatgtt catcaggata catttcaaaa
      601 tctaaatcat gtaaaagtcc aactatttcc caaaattcta ctctttcagg gtcgtactcc
      661 tttgcatagt atctcataac tccagataca atttgagcat gtcttatatg aaattcttcc
      721 ttattgtatt ttcttaatat ttcccatgct tgttctaaag ttggcatgta actcattttt
      781 atcactcctc tagctgaata ttttgaattt ttataaagtt tattttccat tgattatatc
      841 aatcaatttc taaaacgtca acatactgat acacaatttt gcatttaaat tcagaaatta
      901 gtgaaaatta aatctttata tctaaattct ttgtatctaa agattttcgt tcctcaatgt
      961 tatctttgat acaaatgcta taagtaatgt aataaatcca actcctagta taatgatgta
     1021 cactctatct gataataaat caaaaagttt accatacata acctgaccaa ggggcattgc
     1081 aattgctgtt cctgcaaaaa gaattgacat tacttttcct aagagttcat ttggagtttt
     1141 taattgtgtg actgttataa catatattga gataagagtc gttgtaaaaa atattagcat
     1201 agatgatact ataaagatta aaaatggaac tggaaatcct gtgtttaata taaatggata
     1261 tactgaaacc gccattggta tgaacataac tgcacatata ataaaggaaa tatgcaaatt
     1321 agtgattttt agatgttttg agaaaatacc aatactaaca gcagctacca gtgaactaaa
     1381 agaaattgct ccttctgcta gaccatataa gaagttacta gctcccatag tgatttttac
     1441 tatatagggg ataccaacta ttaacataga gcttaaaaat aagtttattg ctatagctaa
     1501 aaaaagtatt ttta
//
LOCUS       DAYAOX010000372         1508 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.377, whole genome shotgun sequence.
ACCESSION   DAYAOX010000372 DAYAOX010000000
VERSION     DAYAOX010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.377"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..292
                     /locus_tag="ACGI57_003663"
     CDS             <1..292
                     /locus_tag="ACGI57_003663"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718707.1"
                     /translation="FNSMASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIE
                     NNALDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            556..810
                     /locus_tag="ACGI57_003664"
     CDS             556..810
                     /locus_tag="ACGI57_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718708.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            855..1178
                     /locus_tag="ACGI57_003665"
     CDS             855..1178
                     /locus_tag="ACGI57_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HFG9718709.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
BASE COUNT          632 a          111 c          239 g          526 t
ORIGIN      
        1 atttaatagc atggcatcaa aacaaatacc aagaaacaat ttagaaaagt ttttaagaat
       61 aagagtctta tcacatacag gcgataaaat acatataaag ttacctgtaa attttgcaaa
      121 gagaatgatt gagaataatg ctttggattt atttaatgga aaagatgatg tagtagatgg
      181 taaaaaagtt actgaaatat tagttaaggc atttgactat gatttaactg gagaagtagt
      241 tcacttggag agaaaaaatg gagacattat taaagtttca attgtacaat agaattattt
      301 tatatgaatt ttatttttga atagttataa ataattagaa atttgtaaaa aagtataata
      361 tattattagt aatggtacag aatacacatt tataatttat agatgtgtat tttttgttta
      421 aaaaatttaa atatataaaa ctataaaggt tacaattttg gaaatagatg taaatatatg
      481 ttgtattgta taataagagt aatataaaat ctagtaaaaa taatcaaata aatttagctt
      541 atgagaggga taactatgga aaataaggat tttaaattaa caatatgtat atttgctttt
      601 gtaattggag tagtagtatt attctgttgt ataggtttag gtgaatttag tgcagagcat
      661 attattcaac aaaatggtgg aatcatggat actgaccaat ataatattta tctagaccaa
      721 tctatttctc aatatagaaa ttttggttct atattagctc tgttaggagg aatagggata
      781 ttgtttgata aatatatcag aaaatactag agattatgtt ttgtctaaaa taaattctta
      841 tgaggagata aaaaatggca acagaaatat ttactgtaaa aattacaccc ttagaggcac
      901 aaaaaatgat aaaagaaaat cttgatgcag atttagtttt tagtgattct tataatttag
      961 gagatgataa atttatacta attacaactt ttgagaagta ttatgcaaga aataatagtg
     1021 atgcaggatt aatagtagtt tatgaaaata ccacaggaaa aacagttatt aaagctactt
     1081 caacaggcag tgcaattgga attttaaaaa ttgattgggg tgcaggtgga aatttactta
     1141 aaagagtaaa aaacatactt gctgattata ttatttaatt attttatcta actgataata
     1201 tgtcaagaaa atctcgtaaa agaggttaag aacattttta taatatatag ataaataaga
     1261 atttgaaggt aaagttttta aggtataaag aaaaataata taataaaacg aattggcttt
     1321 ataattcttt ataaggcaaa gagcacattt ctttatataa ttgttggata ttaaataata
     1381 tgtaaataca ccatttacat ataaaagttt aattaatatg ttaagagaaa atgaaatgga
     1441 aattactaaa aaatattttt aaattggata atggttgata ttatgtttat acaaaaatca
     1501 tgtagaat
//
LOCUS       DAYAOX010000373         1500 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.378, whole genome shotgun sequence.
ACCESSION   DAYAOX010000373 DAYAOX010000000
VERSION     DAYAOX010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.378"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            341..523
                     /locus_tag="ACGI57_003666"
     CDS             341..523
                     /locus_tag="ACGI57_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFG9718710.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIIF"
     gene            594..1439
                     /locus_tag="ACGI57_003667"
     CDS             594..1439
                     /locus_tag="ACGI57_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9718711.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
BASE COUNT          644 a          170 c          172 g          514 t
ORIGIN      
        1 tgtaataata agacttatct ttacatacca catagttgat ataaaacaat gaaattaaca
       61 tacaagagac taagacaatt aaaatcttta cataccacat agttgatata aaaccccaaa
      121 ataaacttag catctccaat gtttacacat atacaatgct cttaaaattg cagtgaacgc
      181 gcaatagtgc aattgataat acttattata tattctcaat gccttatatt tcaactgcta
      241 aacctcaatt tgtcacaaat atcgctcact gcaaaatttc tatattttta ttatatcata
      301 acatataatt aatattttca attaattttt acatttaatt atgtttaaat tctataataa
      361 tgcaacttta atgtttcttt ctggaactaa tataaaaact atttcagaaa gattaggtca
      421 tgcagatata aaaataacta tgaatagcta ctctcagttt ttagaagaga tggacagaga
      481 agcatttgaa aatttgagca aaatactatt tattatattt taaagaagat gacttattca
      541 atatttgatg ctagaatata attaataaaa atttaattat aggtgattgt attatgaata
      601 ttattaaaag cataacttta ctcttaacat tattaattgt gatattaatg acaggctgtt
      661 ccccaagaga aagtgctagt gaagttgcaa aaaaatatat tgataagatg aactctggtg
      721 actatgaaac agcatatagt ttattaagta aagatagtaa aaaaaaaatt agtatagaaa
      781 atcttgaaga atttcaaaat attttagtta gtacaaaaag aataattagt tataaaatag
      841 gtaaagaaaa aatcataaaa aaatataaac atgatggaaa agaatataaa aatgttgtta
      901 aattagaaga aacttttaat ataaaaaacc ttctttataa aaaagatgat tctctaaaag
      961 taaatagata ttttgtagtt gaaaataaca aatacaagct attattgtat gaaagtttca
     1021 aaaaagaatt tggtattaat agtatggacc ttgctcaact taagcttaat gattttggac
     1081 taaaatactt taaagaagtt gaccttatac taaaaaaagc tattgctatt aatcctactg
     1141 attcaaagct ttattatgcc caagcatgta attatatgaa ttttgatgga tttcctggtt
     1201 attcagatac aacatataat gctttagatt caattaataa atctttaaag tatttagata
     1261 aaaatgattc tgactacaaa aaaaatgcaa gtaatatata taacttaaaa ggagttattc
     1321 ttatgtttca caaaagattt gatgaagcac aacaatattt aaataaagcc ttatacttta
     1381 acaaagataa tgaagaccca aaatataatc taaatacaat aaaaaaacat ctaaactaga
     1441 tatttaattt ttgaatttat aaaatatttt tttatgtata attcagcact gaatcataat
//
LOCUS       DAYAOX010000374         1370 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.379, whole genome shotgun sequence.
ACCESSION   DAYAOX010000374 DAYAOX010000000
VERSION     DAYAOX010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.379"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            31..540
                     /gene="vanZ1"
                     /locus_tag="ACGI57_003668"
     CDS             31..540
                     /gene="vanZ1"
                     /locus_tag="ACGI57_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HFG9718712.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LIPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLVFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(642..836)
                     /locus_tag="ACGI57_003669"
     CDS             complement(642..836)
                     /locus_tag="ACGI57_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HFG9718713.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
BASE COUNT          530 a          134 c          172 g          534 t
ORIGIN      
        1 taatacaata atatagtaag gaggtaataa atgaaatcta gaaaacataa tattacaaaa
       61 ggattattta tagtttacat aattatttta acatggataa tactatttaa gttgcaattt
      121 gatatctcat ctcttgaaac catgaatctt agaagcataa acttaatacc ttttgctggg
      181 tcacttataa ttaataatag agtagatata tcagagatta tattaaatgt ggctatattt
      241 gtaccatttg ggatatatgt gtgtatgcta aaagaagaat ggagctttat aaaaaaagtt
      301 atacctattt ttataactag tttggtattt gaaacacttc aatatatatt tgcactaggt
      361 gcaagtgata taacggattt gataggaaac acactaggag gaatcatagg aatagctgtt
      421 tttatgctat tatcgaaaat atttaagaat aataccataa aaattatcaa tgtattggca
      481 ttgattgtga caattattgt tgtattattt ttaggattag ttatttttgc aaacttatag
      541 aaataagtat atatgtttag cttaaaatag aacgtaagat acagttacat ataaaattat
      601 ttacacataa aaagcaccta tacaggtgct ttcctaaatt attaaagaat tattgtatct
      661 tgaaactctt gtatgttcaa ttcttgattt tctcttgttt tttctaataa atctaacgca
      721 caatttatac aaactttaat ttctgcattg gtatttttct cataataagt tatatattta
      781 tttctatatt tatctatttc ttttccacaa cgactacaca ttacaattac gctcatatct
      841 tagttcctcc atctctaatt attcttaata tattcaatag tatcactttt ctgaaaataa
      901 tgattgttaa aactgtatca aatttagatt tactttttat tataatgcat tttaataaaa
      961 aaataaaggt tatgggtaaa atatttatag aaaaaagtat attattgaat agattataaa
     1021 attttattat tttttagcag tgttaaatac acattaatat ctataaagtg acaaaataaa
     1081 aaatactatt taaatagata ttaaaaaatt gataccaagt atttatttgc tatatgaata
     1141 acaatttatt taggaacttt actttaacac tgtataataa taaagtaatt gctaaaaacg
     1201 tttgatgatt tggatgtatt tttagaaatt agaaaaatat atacttactg actaatgata
     1261 atcttatatg tattttagtt tgagtgattt aagaaaagtg ttttgaatgg ggattatttt
     1321 atagagtaaa taaatatata atagtagaaa attacatata aagagtagtg
//
LOCUS       DAYAOX010000375         1363 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.380, whole genome shotgun sequence.
ACCESSION   DAYAOX010000375 DAYAOX010000000
VERSION     DAYAOX010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.380"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            103..1272
                     /gene="nth"
                     /locus_tag="ACGI57_003670"
     CDS             103..1272
                     /gene="nth"
                     /locus_tag="ACGI57_003670"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFG9718714.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKEEINKKGMNKKDIIEKEEINKKGMNKKDIIEKEEINE
                     KGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKGETEKKTKKSSSNKATQ
                     KTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILSAQC
                     TDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLCELY
                     DGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQK
                     TEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
BASE COUNT          633 a          102 c          267 g          361 t
ORIGIN      
        1 ttattttgta tgttttatat attgatgttt atttttttgt tgatatgatt aaactattta
       61 taaatagcgt tttataaatt ggtattttga agggggataa ttatgaaaaa tgtaaatcta
      121 aaagagataa atgaaaaaga tgtaattttg gaagaagaga taaataaaaa aggaatggat
      181 aagaaaaata taattgagaa ggaagagata aataaaaaag gaatagataa gaaagatata
      241 attgagaagg aagagataaa taaaaaagga atagataaga aagatataat tgagaaggaa
      301 gagataaata aaaaaggaat gaataagaaa gatataattg agaaggaaga gataaataaa
      361 aaaggaatga ataagaaaga tataattgag aaggaagaga taaatgaaaa aggaatagat
      421 aaaatgattg gtaaaaaaga tgagataatt gatgagaaag atatagagaa ttttatggat
      481 attgaagaag ataaaaaaag tatcaaagta agtaaaaaaa gtactaaaaa aggtgaaacg
      541 gagaaaaaaa caaagaagag tagttctaat aaggctacac aaaaaacaag taaagaaact
      601 aaaagtaagt ctaaaattaa aaatgagtca gcaaatgaaa aagacgtaaa taagatttta
      661 gacgagttag aaaaattgta tccagatgct aaatgtgagt taaattatgg gacagccttt
      721 gaacttttaa tagcaactat tttatcagca caatgtactg atgtaagagt gaataaagtt
      781 acttcagagt tgtttaaaaa gtacaacaca gctagagact ttgctaattt gagcattgaa
      841 gaaattagta aagagataaa gagttgtggt ctttacaaaa gcaagtctca aaaaataaag
      901 gatacatctg aacagctatg tgaattatat gatggtgaag tacctgatag cttagaaaag
      961 cttataaaat tacctggtgt tggaagaaaa acggcagggg ttgtacttag taacgctttt
     1021 aatcatccag caatagctgt agatactcat gtatttagag ttagcaatag aataggaata
     1081 gtggacgagc caaatcctca aaaaacagaa tttgcactta tggaagctat accaaaagaa
     1141 agatggtcac actcacatca tgtattaatt tttcatggaa gaagaatgtg taaagctaga
     1201 aatccagaat gtgcaagttg cccaataaaa gaagattgta attattataa agaacttaat
     1261 gaaacgaaat aacttttaga aagtaagttg tacaagaggg attttctgat ataataatac
     1321 ttgtgaaaat gttagataaa aaactattat atacatgaaa taa
//
LOCUS       DAYAOX010000376         1278 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.381, whole genome shotgun sequence.
ACCESSION   DAYAOX010000376 DAYAOX010000000
VERSION     DAYAOX010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.381"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            80..721
                     /locus_tag="ACGI57_003671"
     CDS             80..721
                     /locus_tag="ACGI57_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HFG9718715.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT          515 a          115 c          216 g          432 t
ORIGIN      
        1 gtaaagaaat ttaattataa gattaatttt ctgaagattt agtatttgta ctaagaaata
       61 cataaaaagg ggaatagata tgaataataa ttttcaagac aaccaaacaa taaatttaat
      121 acaaagtcgt agaagtatac gaaaatttac gacagaacaa attagtgatg agcaagtaaa
      181 cacattatta cactgtgcat ttgcagcacc ttcaggatgt aataaacagc cttggcacat
      241 aactgttgtg caagaccaga aacttttaaa agagattagt gatgatactt tatctaggat
      301 tcatgaggtg agtaatgttg agataaataa aaactttaag ttattttatg gtgctccaac
      361 cgttttattt atttcttatg atgaaagtag ttcatgggct ccatatgata taggaatact
      421 tacagggaat ataacaacag ctgcacaagc actagggctt ggaagttgta taataggaat
      481 ggttagaggt ttatttacac ctgttgaaca aggggatatt gaaggtcttg tgagtgtatt
      541 ggataaagaa gatgtaaaag aatcagagtc aataaagatg aagtttgata caaataaaaa
      601 atacagagaa ctacttgata taccagaagg gtattctgta ccatttggta ttgctgtagg
      661 tattccagat ggaaatttac caaatgctag agaggtagtt tacaaagttt ccagagttta
      721 ggataaagta ttgcaagaac caatcagtta cttgaagaat taaatcagta atatacaaat
      781 aaattgatgg aagaggaagc tatttaaaaa gtagtttaga tttttcgttg taaaataatt
      841 aaataaagaa taaggtgata agtattttat ttattatctt attctttttt atttttgttt
      901 tgtagaatga taaaaataag gattaagtgt aataaaatag aaaacaagta tgttaatttt
      961 aagtatatgc tttaaaattt atccaagtta aaaatttaag atatcttttc agtataatgg
     1021 aaaaaatgtc aaaatattga aatagggaaa aaatagtgat ataataagaa aaaatagaga
     1081 aaaaaagtaa cccttagtta taaaaaagtt actatttatt gaaaatttag atatttttct
     1141 aaaaattatc tatgttaata gttaaattta tgaacatttt ttgtttttat gttaggatat
     1201 ttcttagaaa tgaaatactt cttaatttga tttgatatgt atatttttat agctatttta
     1261 ttataaaaaa aaagaacc
//
LOCUS       DAYAOX010000377         1277 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.382, whole genome shotgun sequence.
ACCESSION   DAYAOX010000377 DAYAOX010000000
VERSION     DAYAOX010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.382"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(64..276)
                     /locus_tag="ACGI57_003672"
     CDS             complement(64..276)
                     /locus_tag="ACGI57_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9718716.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(277..693)
                     /locus_tag="ACGI57_003673"
     CDS             complement(277..693)
                     /locus_tag="ACGI57_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718717.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(761..1174)
                     /locus_tag="ACGI57_003674"
     CDS             complement(761..1174)
                     /locus_tag="ACGI57_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718718.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
BASE COUNT          494 a          196 c          121 g          466 t
ORIGIN      
        1 caaacttaat aattaaataa ctatatttta ttatccatct tataaatgtt tataaacttt
       61 ccactattct tcttcaataa attcaaacaa attttctact gtagtatcga atactttagc
      121 gatatccata gctaacttca aagaaggatt atatttccca ttttctaagt gtacaatagt
      181 ctcacgtctt accccaacca atttagcaag ttcattttgc gtcataagga gtttttctct
      241 gtattcttta atttttgttt tcatcaatat tgccacttag ttacatatcc cccttttatc
      301 aaagtatata tacaacacta atttagataa agactgtata aataaaactg caatgaggat
      361 tatacctgta tctaggtttg atatatttat attacttgta taaggtgttg caaagaaaat
      421 agatattatt actatagaat atatcattat ttttattgca atcttattaa cctttgatag
      481 tataaattca gatgattcat caactaaatc attatatttt ttattataaa ttgaataaca
      541 tccaaaaact attaaccaac tcaaccctgg aagagaagca gtgcttggac ctttaaaaat
      601 atacttagtg gctaatacag ctataaaaaa ataagttatt acaagcaact ccaggataag
      661 ctttgatctt acaccagttt ctgatttaga catataaaat cactccttaa aaattatatt
      721 ctctatactc tttaattttc gtttttatca atattatcac ttagttgcat attccctttc
      781 tatcaaaatg tatatacaaa attaatttag ataaagattg tataaataaa atagctaaga
      841 gaattacacc tatatccaaa tttgatatat ccaaacctct tgtataattt gctgaaaaga
      901 aaacagctat tagcaaaata gaatataaca ttatttttga tgcaatatga tcaaccttta
      961 ataacataag ttttgacgcc tcgtcaacta aatcattata ttttttatta tacatgaagt
     1021 cacatgccca aaaagcgaat aaaattataa aagtaataca aagatttccc tctttaaaaa
     1081 taaacttttt taataatgag tctataaaaa aataggatat cataagtaac tctgaaacaa
     1141 gatttgtttt aacaccaagc tctgatttag acatataaaa tcacccctta aaagttatat
     1201 atttataaca ccttatgtta taaatatata acttttccaa ataaattgca atagaatatt
     1261 gaaaatattc aataata
//
LOCUS       DAYAOX010000378         1261 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.384, whole genome shotgun sequence.
ACCESSION   DAYAOX010000378 DAYAOX010000000
VERSION     DAYAOX010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.384"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(237..1106)
                     /locus_tag="ACGI57_003675"
     CDS             complement(237..1106)
                     /locus_tag="ACGI57_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFG9718719.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          480 a          187 c          110 g          484 t
ORIGIN      
        1 aattctaatc aaccgtaaat aataatctaa aatacatcaa aaaataaact aaaaaatatc
       61 gaaaaataat ttaaaagaca tcaaaactaa atcttctaat cagcaattaa aagtctattt
      121 tcttagttta aaaaataaag atagattttt tcaaaacact ggataaatag gtaaaaaaat
      181 aaacagttag taaaataaat ttttactaac tgtttatttt tattgtttaa tatgtcctat
      241 aaatcctcta agtaatattt tccttgcaaa aattctgatt ctaaacctag atttttaaaa
      301 taatcaacca tgcccataca cgcaaatttt tctgtagaat aatgagtagc acctataaca
      361 ttaattaaat tatcttttgc tatttgatgg aactctaata ctggttcaaa atgttttaaa
      421 ggcttagtaa acccagtaat atatagattt ataccaagtt cagcaacttc tcttgctaca
      481 aatggataac ttcctccccc tgctgcaata gcaactaaac catcttttaa tacagaatct
      541 ccataatcat acaatttcac atcatgacca acagattttt taattaattc cttaatctct
      601 tctattgatt ttagtgatgt tttacatata actcctacct caattttatc gtcatattta
      661 caaaatggtt tgactatttc taaacccata gcttttgcaa aactaacaga agttgaatag
      721 tcactatagt tgtctagtgg agaatgtaat acataaaaag aaattctacg atttttcatc
      781 tcttccatat aagatagtgg tatgtcgtga aatggaaaac cttcatctga tgctatgtat
      841 cccattgcat gatgtgaaaa aattaaaata tctttttcat ctttatctat aacttgctta
      901 attatttctt tatcaggaaa agtagttgtg tatactttat taatattttg agcattatct
      961 aatactaatc ctatgtattt ttttctaaat tctggagtaa taaaatcatt ttcaaaattc
     1021 ataaaactcc aatcatcatt aatattttta atattaaatt ctaaatctaa ttttttataa
     1081 agttcaattg ccttaataat aaccactcct ttataataat attgaattac tacatattaa
     1141 tatagaaatt tccgataata aactttatac gtttaagtat tcctacttca aattaaaaat
     1201 attataaata ttcaaattta ttaatatatt ttaatattat atttattttt tctttattaa
     1261 t
//
LOCUS       DAYAOX010000379         1246 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.385, whole genome shotgun sequence.
ACCESSION   DAYAOX010000379 DAYAOX010000000
VERSION     DAYAOX010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.385"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(402..1133)
                     /locus_tag="ACGI57_003676"
     CDS             complement(402..1133)
                     /locus_tag="ACGI57_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9718720.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
BASE COUNT          489 a          169 c          108 g          480 t
ORIGIN      
        1 tatcaaattt ataaatatct gtatttatat cactagtaaa tatcaagtaa tttaatggag
       61 ctgtaaatcc atatactaat tgttcaatta aatatactaa caaagttgat actatagata
      121 aaacagtaga ttgttttatt atgtacttta caaataaaaa agtcataaga aaaaatagaa
      181 tctgtgaaga aaataaatta tataaattaa aatatgtatt tataatgatt aaaaaaattg
      241 aaattatcga atataaaata aaacaccaaa attttgaatt aaaatttgta aaattgatta
      301 gaaaatagta ttgcactatt accattaaaa ttctatatac aataaaagag aagaaaaaac
      361 tgtactttaa aaataaatcg gaagaaaata aattaatcat cttatatcct cctatttttc
      421 atgaactcaa gcataacttt tgaaaatatt tgttgacgta gtctagaaac aggtattcta
      481 tctccacttt ccataactgc attaccattt tcatatccat atacatattg taaattgatt
      541 atataactac gatgacacct aaaaaaattt gttggtaaac ttctctctaa attttcaata
      601 gtctcataat aatctacagt ctctttcttt aaatgaacat aaatctttct ttttataact
      661 tcacaatata aaatatcagc aagttttaat ttccaatatg aataaccttt ttgtattgtg
      721 atataattac tttcattatc atcaagtttt gtaactattt tatccaaagt tttatatagt
      781 ttctcattag atagaggttt tatcagataa tttatagcat ctacttcaaa agcatcaaat
      841 acaaattcat ttaaaactgt aacgaaaatt ataaagcact cattaccata tgaccttaat
      901 tttctagctg cctgcattcc gttaatctct tccatctgta tatctaaaaa tacaatatta
      961 aacttttcat gattgcatag taaatcttct actttattaa aaagctctat attatataat
     1021 aaattcttct gcttcatgta taattctata ttttccttta aatgatttag catataggat
     1081 tcatcatcac aaatggcaat tgaaatcaat atatacacct tctttcttat cattattcta
     1141 attattatat aaagttaatt ttttctgttc aattaatatg atctaatatg ttgaatattt
     1201 gttccaaaat gtaataatct cgcttttcat agtatatcta atttga
//
LOCUS       DAYAOX010000380         1245 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.386, whole genome shotgun sequence.
ACCESSION   DAYAOX010000380 DAYAOX010000000
VERSION     DAYAOX010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.386"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            116..1156
                     /locus_tag="ACGI57_003677"
     CDS             116..1156
                     /locus_tag="ACGI57_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFG9718721.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLSLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          477 a          145 c          198 g          425 t
ORIGIN      
        1 ttttatatac ttttttttta aatatgttta gattctattc ttttaatttt agttttcaga
       61 acatttgtat ttatgttata atttgtataa atatatttat ttaggaggaa acaatatgaa
      121 tttaaattta actaatacta taaatttaat gaaaaaatat ttggacattc caagcccagc
      181 tggatatact gaaaatgcag ttttagaaat caaaaaagat tttgaaaatt taagtcttga
      241 cactattctt acaaaaaaag gtgcacttat agctactctt cctggtgaag atgattcaaa
      301 tcaaataaca atatctgcac atatggatac acttggagct atagtaaaag aaatatcatc
      361 tgatggtact cttaaatacc ataaagttgg tggtggatgt tgggcggcta tagagggaga
      421 aaactgtact gttataacta gaaaaggtaa aaaaatcaga ggaagtgttg tatttaagta
      481 tgcttctact catatatatg gtcaaacaaa agctgccact gaaagaaatg aagaaacaat
      541 gattattaga cttgatgaaa aagtatttac aaagcaagat gtattagatt taggaataag
      601 tgttggggat tttgtatgcc tagaccatag atttgaggct acagaatctg gttttataaa
      661 atctagatat atagataata aatcagctgt agcaatggtt cttgaaatat gtagatattt
      721 taaagaaaac aatttaacac ctaagcatac tactaacttt tatataagta attatgaaga
      781 aattggacat ggtgtttcta aatgtttacc tgaaaaaaca aaagaatttg tagcaataga
      841 tatagcacct actggaccaa gtcaaacatc tagtgaacat ggtgtaacta tagttgcaaa
      901 agataattta tcattatatg attttaattt aagaaacaag cttgtcgatg ttgcagaaga
      961 caataattta gactacgctg tcgatgtatt tactttctat ggttcagatg cttcagaagc
     1021 tattcgttgg ggagaagatg ttcaaatagc atgtgttggt cctggaacaa acaatagtca
     1081 ccattatgaa agaacacata tagaagctat tgaaaataca ctaaaattac ttataaatta
     1141 tatgcttaaa gaataattaa ttatatattt taccaaagtc aattcttaac aaataattaa
     1201 ctttaatttt tatttttata caaaaattgg tttttctata gactt
//
LOCUS       DAYAOX010000381         1216 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.387, whole genome shotgun sequence.
ACCESSION   DAYAOX010000381 DAYAOX010000000
VERSION     DAYAOX010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.387"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            127..438
                     /locus_tag="ACGI57_003678"
     CDS             127..438
                     /locus_tag="ACGI57_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFG9718722.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            466..1152
                     /locus_tag="ACGI57_003679"
     CDS             466..1152
                     /locus_tag="ACGI57_003679"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFG9718723.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT          469 a           95 c          202 g          450 t
ORIGIN      
        1 taaaaatata tctgaatttt actaacttat ctaggggtta tgtacaagga ttttaaagat
       61 aaatatagaa attaatgttt ataatcgata cataacttga gtaacaaaat ttaaggaggt
      121 taaagtatgg gtatatttaa aacaaaaatg gatgaggact ggaaagtaaa ctacattaaa
      181 gagtttaatg aaatgagaga ttcttatgag agtaaacttc aaaaaaaaca gtttgaggta
      241 gatagtttga aatcagaatt ggatagacta agaagctata aaaatagcct taagccaaaa
      301 gaaaaacaaa ttactgatga tgatataaat aatataaaga gtttaagaag agatggctta
      361 agctataaag aaatttcaaa tcaaacaagt tggagcaaag caactgtaag tagggtatta
      421 aatggacttt acgattagca tatacatggg gtgatttaaa ttgggatgaa gtatagttac
      481 aaaaaatgca taatagattc tattttattg atgtttatag ttcaaatatt aaggatgatt
      541 ttgaattatg tacttctaag tcaatttgaa tttactcttg aaaattttaa tattataaac
      601 ttaatttctt ttacattagt tggtttatcg ttaattttat ttttaaaaga taattctttg
      661 tataacaagg ttagaaatag aaaaatcaca gaagcttttg aagaaaataa aaacaatata
      721 cttattgaaa agtgtaagct gatacttttt gttgtggttt tatctttagc aataattgta
      781 acttattgta ctaagggata tgtacttttt aatgtaacta tgatgacttt gtctgtattg
      841 atagtcccaa tatttgaaga gttatttttt agagaatata tatggaacta tctaagtaat
      901 tttataaagt caaagagtaa aattatctgt ataacatcta tactatctgg aatttataat
      961 ataggataca tagatgttat aagaaattat gtaatattgt ataacaattc aagctacacc
     1021 tttgaagtta ttatttcaaa aattatgata ggtactgttt ttggtattgt acttggatta
     1081 gttaagtata ggtttagaga tgtagggttt tgtatattat tgaggagctt atttgctata
     1141 tttattaggt agatagtata atcggtttat aaggttgttt taaatttaat tttaaatcaa
     1201 ccttatttta tttgtg
//
LOCUS       DAYAOX010000382         1215 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.388, whole genome shotgun sequence.
ACCESSION   DAYAOX010000382 DAYAOX010000000
VERSION     DAYAOX010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.388"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..976)
                     /locus_tag="ACGI57_003680"
     CDS             complement(<1..976)
                     /locus_tag="ACGI57_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFG9718724.1"
                     /translation="MFITVVLFLVGFLLITKGADIFINCTVEIGKKTNISEIILGATI
                     VSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICNTGLALGLVVFISPFNVDKKMFF
                     SKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIFYMVNNYRSVVGKSDTKNRNIKN
                     NTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMMIIGSQILIESGVIIASFLNIPQ
                     GIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNILGANILNIVSVIAVSAIPNNIP
                     ILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGILMLFTYFLYISIL"
BASE COUNT          439 a          180 c          128 g          468 t
ORIGIN      
        1 acaaaattga tatgtacaga aaatatgtga ataacatcaa tataccttgt attctactaa
       61 gtttattaga tttaagtgtt ggtattatta ctattaataa taataatatc atgaatggta
      121 tatccaattg tctattttga gatagtattg gtatgttatt tggtattgct gatacagcta
      181 ttactgatac tatattcaat atgtttgcac ccaaaatatt tcctactgat atttcatgat
      241 gattttttct tatagcagta atcgacgaaa ctatctctgg cagagaagtt ccaagtgcta
      301 ttatagttaa acttactata ccttgaggta tatttaaaaa actagctatt attactccac
      361 tctctataag gatttgtgaa cctattatca tcattatcaa tcctgtagca aaaagtaata
      421 gtatttttac aatttctaat acggaaaaac ctctataatt tttccctttt ttagtagata
      481 tgtctttaat agtgttattt ttaatatttc tatttttagt atcagatttt ccaacgacac
      541 ttctataatt attaaccata taaaagatta atattattat aagtaataat gaatcacctc
      601 ttgttataac tccatctaaa ctcaataaaa ttaaaactat aacggatact attaatagta
      661 aagacttaga aaaaaacatt tttttatcta cattaaatgg acttataaaa acaaccaacc
      721 ctaatgctaa ccctgtatta catattatag aaccaactgc atttcctaaa ctcatagtag
      781 tatgaccatc aatagaagca agtagagaca ctgtaaactc aggtaatgtt gttgcaaaac
      841 taactatagt agctccaagt ataatttctg atatattcgt ttttttacct atctctacag
      901 tacaatttat aaagatatct gcgcctttag taattaatag gaaacctact aaaaataata
      961 ccactgtgat aaacaatcta tcacctcccc ctattaaata aatattcagt ttgtgaagat
     1021 gtatgctttt tcttttttcc ataataaaat ttatttatcc taaacaaaaa ataaaaaata
     1081 ttttctcctt aattagctat ttatttgttt tattttattt atatatgggt ataatttcac
     1141 cacattatta acatataatc tgttttatac tttaaagcga caatttttta ttttttatgt
     1201 atacaggata cgaat
//
LOCUS       DAYAOX010000383         1208 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.389, whole genome shotgun sequence.
ACCESSION   DAYAOX010000383 DAYAOX010000000
VERSION     DAYAOX010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.389"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            135..1202
                     /locus_tag="ACGI57_003681"
     CDS             135..1202
                     /locus_tag="ACGI57_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFG9718725.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
BASE COUNT          517 a          113 c          202 g          376 t
ORIGIN      
        1 ttatttattt taaagaagtt tattttaaaa ataaaattaa atttaaaaag ttatgtattg
       61 attatattta aaaatgttat actctaaaaa agtatatttt atttaaaaat ttattaatac
      121 aaaggagatt tactatgaaa aataaattag tttacatatg tattatgagt ctattgctat
      181 ttgtagtagg atgttctaat aatccaaata aggaaagtac cattaaaaaa gactctaaga
      241 ataaccaaag ccaagttaaa gaagaaaaaa gagaacctgt gaaaaaagat ataactataa
      301 gtttcgctgg tgatgtaact atggggaact ataaaggttc atcatattat ggcagttttg
      361 accatgaatt taaaagacaa ggagaagatt acaattattt tcttaaaaat gtaaaacctg
      421 tatttgaaaa agatgactta acagtggtta atttagaagg tccacttact acagcgtcaa
      481 atgcaaaaga aaaaaaattt gcatttaaag gaaagcctgg atatgtaaat attttaaaat
      541 ctggaagtgt agaggcagta agtattgcaa ataatcattc agaagattat tttgaagaag
      601 gaatgaaaga tacacagttt atattggacg agaataaaat aaattatttt ggattaggga
      661 aagatgctgt tgtagatgtc aaaggaatta aagtgggtct attagggtat aatggattat
      721 caactgaata taatgaaaaa aatttaaaac aaatggaaga tgatattaag tccttaaaaa
      781 agaaatcgga tgttgttgta gtgtactttc attgggggat agaattagat tattatccag
      841 ataaaaaaca aaaagatttt gctcattatg ctatagacaa gggagcagat ttagttatgg
      901 ggtctcaccc acatgtgatt caaggaatag aaaagtataa aaataagtat atagcttata
      961 gtctagggaa tttttgcttt ggaggaaata agaatccttc tgatacagat agttatatat
     1021 atcaacaaac ctttactttt tcagataata aattaacttc aattaaagag ccaaatataa
     1081 taccaacttc aataacatct agtaactcca gaaataatta tcaaccaaaa atattggatg
     1141 gaagtgagaa ggatagagtt ttaaataaac ttgaaaaaat aagcaaagat ttaaataaat
     1201 aaaatgta
//
LOCUS       DAYAOX010000384         1144 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.390, whole genome shotgun sequence.
ACCESSION   DAYAOX010000384 DAYAOX010000000
VERSION     DAYAOX010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.390"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            59..217
                     /locus_tag="ACGI57_003682"
     CDS             59..217
                     /locus_tag="ACGI57_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718726.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     repeat_region   503..1061
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=503..532
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaatt"
BASE COUNT          406 a          140 c          183 g          415 t
ORIGIN      
        1 gtggagttca tgtatcaaaa aattatcctc ccgacgttaa gaagggaggt gaatatacat
       61 ggataatttt ttacaaggca tactagcaag tctatctgct agcttaatag tttatatagc
      121 tagcaaacta tttagaaagc gtaaaaaacc actcaaagcg gcaactaaga gtggttggga
      181 atttgattta aaaatcagat tccataaaac taagtaattt ctaaattatg aactccactc
      241 tacagcaaaa tagattgtag ttcttcttgc ttttattata tcacaaattg gtacagatat
      301 tcaaaaataa tatttttatg atataataaa gtcatagaaa ttttgcagtg ttcgattttt
      361 tgaagaaatt aaggcttaac agttgaaata taaggcattg aggacttgtg ataagtgtta
      421 tcaattgcac tactcatggt tcactgcaaa tttgagagat ttgtatatgt gtaggcattg
      481 gaaatactca atttattttg gggttttata ttaactatat ggaatgtaaa tttcatattc
      541 catgcattct gcttacactt aatagaaaag ttttatatta actatatgga atgtaaatta
      601 tttctagcga cttggtcggt tgctccattt agcttgtttt atattaacta tatggaatgt
      661 aaattttttc aaatctgata tatcgacctt aaatttcgtg ggttttatat taactatatg
      721 gaatgtaaat gatataaact ttgggagttc agaagaagga ttaaaaagtt ttatattaac
      781 tatatggaat gtaaattaga gatgaactca gatagaaaaa catgtagatg tgtgttttat
      841 attaactata tggaatgtaa attcttcaac agttgtgtta ccatctccta cacgcacagg
      901 ttttatatta actatatgga atgtaaattg ctacgtaacc agtttcaccg ctttcagtat
      961 ataacgtttt atattaacta tatggaatgt aaattgttgg tatagtttca gaaaagaaat
     1021 tcgaaacact tgttttatat taactatatg gaatgtaaat tgtatccaaa aaattacaat
     1081 aatcatacga actgctaata taagcagtca atatatgtgt acatttatct attttatctc
     1141 taaa
//
LOCUS       DAYAOX010000385         1141 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.391, whole genome shotgun sequence.
ACCESSION   DAYAOX010000385 DAYAOX010000000
VERSION     DAYAOX010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.391"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            37..>1141
                     /locus_tag="ACGI57_003683"
     CDS             37..>1141
                     /locus_tag="ACGI57_003683"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9718727.1"
                     /translation="MKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGK
                     NEVVILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLM
                     PYIISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITII
                     SGQIEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINI
                     SELLNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEES
                     ELIIRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGG
                     SGLGLYIVSQIFKSHGFDYSIKNKENSVVFTVEF"
BASE COUNT          495 a          119 c          189 g          338 t
ORIGIN      
        1 tatcatgagt ataaaattga gtctcttcaa gaaagtttga aatctttagt agatagttca
       61 attcatttcg atacatatac tttagaagaa agactttatt atatggctaa agaccaaaat
      121 ttggctattt tattaaaaga taaccaaggt aaaattgtat atggtaagaa tgaagtagtt
      181 attttaagat atagtaagta catgattaat agtcttgagg atgagtatag aacttctata
      241 cctatatata ctaaagatgc aaaagatggt ccatatactt tagaactcgt tatgccatta
      301 caaccaattg acgaagcaaa cgaagttata agaaaactaa tgccatacat catatcaata
      361 gcaatattga tagcgattat tggagcatat atatattcaa ttgtaataac aaagcctctt
      421 ataaacataa tagagagtga gagagaacag gaatatagaa ggaaggattt tgttgctaca
      481 atatcacatg aactaaaaac tccaataacc ataataagtg gtcaaattga aggtatgatt
      541 tatagtgtag gtaagtataa ggatagggat acttatctga aaaaatcgta tgagtgtaca
      601 caagaattaa aagatttagt aaatgaaatg atagaggttt caaaatcaga aatacttgaa
      661 aaagatttga aattggtatc aataaatata agtgaattat taaatagact tgtaaagaga
      721 caagtatttt taatagaaga aaaacatatg aaaactattt taaaaataga agaaaatttg
      781 gaaattaaag cagaccaaga gagaataact aaggcaatca ataatataat aaataatgcc
      841 ataaaatatt ctcctgaaga atcagagctt ataattagac tctatgataa aaataaacga
      901 ataagtaaaa agacttctaa tcaaagagtg gttttagaaa ttgaaaatac tggtgtaact
      961 atcgagaaac gttatttaga agaaatattt aatccatttt atagaataga aaaatctcgt
     1021 tcgagaaaaa ctggaggaag tggacttgga ctttatatag tcagccaaat ttttaaaagt
     1081 catgggtttg attatagcat aaaaaacaaa gaaaactctg tagtattcac agtagaattt
     1141 a
//
LOCUS       DAYAOX010000386         1123 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.392, whole genome shotgun sequence.
ACCESSION   DAYAOX010000386 DAYAOX010000000
VERSION     DAYAOX010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.392"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(242..988)
                     /gene="cdtR"
                     /locus_tag="ACGI57_003684"
     CDS             complement(242..988)
                     /gene="cdtR"
                     /locus_tag="ACGI57_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HFG9718728.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT          416 a          137 c          117 g          453 t
ORIGIN      
        1 aaaataacaa ctgaatattt taaagagact atttcgtact tttatgattt aatgagctat
       61 cttatcgaat cataatctag gaacaattgt tataatatct aatagtagtt atatatgata
      121 tagtcagtat gtgttttttc acaatatgat tattatatag aggattctta gggattactc
      181 tttttgtata aaaaagagat ggttcaaatt tttatcttga gacatctcct ttttctattt
      241 attatgtttt aataatattc tttaaaatat ttctatatgt attagatata cgacatatat
      301 ggccattact cattttaata ttatttttct ttaggtcgag agaaacaata tgatatttat
      361 taactacaaa agatttatgt acacggataa agcatgcatc taaatctggt aaagacttta
      421 aacttttata gaattcaaat attctagaag tagtcactaa ttttatttta tgtggaatag
      481 tgcttgtttc aaaatatata acttcatcaa atgaaatttt ccatagtgag aaatctgaat
      541 agatagtaaa aaaattagag cgataatttt tttctttaat gtatcttttg tgagcaattt
      601 taatacaagc ttttatttta ctttcagcaa tttccatatc atgttttagt ataaagtcca
      661 taacttctaa cctataatcg aagatcccta aaatcatttc tatatgattt gtaagaaata
      721 tgattggact aatataatct gattctctaa ttttttgtgc tatccataat ccatcacata
      781 attcattact tatcttatat tttgcgtcta caactataaa atatattctt actttatttt
      841 tttcagtaag ctcttttaat aacaaatttg cattatgata taatcttaca acatcattat
      901 caaaaccttc ttttattaaa atattattta ttttttcttt tagtttcatt ccaaaacaaa
      961 catcattatc aaagattaat atatccacta aataccctcc tataaaaaat tcaaaagtaa
     1021 aaaaacaaaa gtattttata gaaaatacat aaaataacac taggagataa ataagattaa
     1081 ggatagttag aattaaggta tatttatgct tgtttatata gta
//
LOCUS       DAYAOX010000387         1119 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.393, whole genome shotgun sequence.
ACCESSION   DAYAOX010000387 DAYAOX010000000
VERSION     DAYAOX010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.393"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..890)
                     /locus_tag="ACGI57_003685"
     CDS             complement(<1..890)
                     /locus_tag="ACGI57_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HFG9718729.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEY"
BASE COUNT          393 a          157 c          139 g          430 t
ORIGIN      
        1 aaatactcac ataatctgtt tagaaattta tcttctaatg gggaaaaagt tttcttttta
       61 gagtaaatga agaaaaactt tctagtaaaa tcaacatcct ttattttata acattttatt
      121 ttacctgcat tgagataatc gatagctgaa atgtacgata taaaggacac tccaagacct
      181 aatctaacca tttccttaat agcctcattt gattctacat gagcgattac atttaattta
      241 cttactggaa agttattttt actaagtgtg tttagtatta agtttcttgt acctgaccct
      301 tcttttctca taataaaatt tagataagcc aattctccta tatcgataca attattttta
      361 ttatcaattt taaaatctga aggagtgata agaacaagtt catcatctaa taagtctagg
      421 tactctattt gaggattatt gattttagaa ccaactaatc caaagcttat tcgttcattt
      481 aaaatttctg atatagcata ttgagaatca taatggctta tggaaaattt tacatctgga
      541 taactcatag aaaaactctt cataaaatca ggtaaaatat aagtttcagg tatggaacta
      601 catgctatat ctataatacc ttctattttt cctgaatatt cttttatatc ataaattgca
      661 cgtttgcaat tattcaatat atatatagca tgttcataaa gaatttcacc agattttgtg
      721 agtgtaataa ctttgttgct tctatttata agaacagttt ctagctctct ctctaaattc
      781 tgtatgtgtg cgcttacagt aggttgagtt aaaaaaagtt ctcttgcagc ctttgaaaaa
      841 ctctgatgtt ttgcaacagc aacaaatact tcaagttgtt taaaatccat tatttaatcc
      901 tccttaaatt atctaaaact attataacat atttgataaa ttaatagtca aaggtgattt
      961 ttaaaataga aaacctttaa atattcatgg agtttttgat tgtttaggaa taatttggta
     1021 taatgttgta aggtacttac aataaaaata tcgtaaattg aattttcaaa attcaacttt
     1081 aaagtgtttt ttattacaga aaaattacaa ttagcttct
//
LOCUS       DAYAOX010000388         1110 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.394, whole genome shotgun sequence.
ACCESSION   DAYAOX010000388 DAYAOX010000000
VERSION     DAYAOX010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.394"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            171..992
                     /locus_tag="ACGI57_003686"
     CDS             171..992
                     /locus_tag="ACGI57_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HFG9718730.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT          443 a           99 c          182 g          386 t
ORIGIN      
        1 cattaggaga agtatcctta atgattttct aaaatcagaa ataggtgctt ctcttttttg
       61 taaaaataaa agtatgtaaa taaaatttaa ccattataat tttcttttaa caaatgtata
      121 caataaactt gttgtataat taagatagat aaaataaagg gagggataat atgaatttaa
      181 tagataatca tattcatact aatttttcaa gtgatggaaa ggattccatg gaagatacaa
      241 taaaaaaagc tataagtatt ggtgttagat atttgacttt tacagaccat ttagaacatg
      301 atgaagatag aggattttgc ataaattata atgactatgt tccagtattt aataagttta
      361 aagaaaaata taaaaaggac atagagttat tactaggtgt tgaagtgggt tacagaaaac
      421 atctaaaaaa tgaaatagaa gaaataataa attctaatcc ttttgatttt gtattgtgct
      481 caactcatac aattgacaat gtacctgttc catcaaaggc gtattttaaa ggtcttagca
      541 aagaagatgc gtattataaa tattttaata gcatacttga aactaatcgt gaatttggag
      601 attacaatat atatggtcat ttggattata tatctaggta tggaatatat tcagataata
      661 gagttatata caatgacttt aaagatgtta ttgatgaggt cttaaagtca ataatcaata
      721 atgggagtgg tatagagcta aatacttctg gttatagata tggactaaat gctatacatc
      781 caaatgaaga tattctaaaa agatataaag aacttggagg atttatagtt actgtaggtt
      841 ctgattctca tagggtagaa gatatatgca aagattttga tgtggcctat gatatgctta
      901 agtatcttga ctttaaatat gtatctttat ttaaagaaag agaaacctat tttgtaaata
      961 tagaaaaagt taaatctaac aatatagctt agagtgtgtt ttgaagtaaa ttaatagata
     1021 gttaaaaagt taatagttat agtattgtat cctctagaaa aatactagag gtttttattt
     1081 tgctaaaata actaaataga attaaattta
//
LOCUS       DAYAOX010000389         1079 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.395, whole genome shotgun sequence.
ACCESSION   DAYAOX010000389 DAYAOX010000000
VERSION     DAYAOX010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.395"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            143..>1079
                     /locus_tag="ACGI57_003687"
     CDS             143..>1079
                     /locus_tag="ACGI57_003687"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9718731.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYII"
BASE COUNT          436 a          116 c          156 g          371 t
ORIGIN      
        1 atatttgaat gctttaagca tgtttactaa ttgtaatttc cacacaaatt tgataatata
       61 tgaatatatt atggtgaaat ttatagatat atattaatta tggggagatt gtgggaggtt
      121 cttttcacaa gacatttaag ctatgaaaat aaataaaaaa ttcaatagag ttgatatgca
      181 ggtttcaata cttactgcaa ttattgtaat aatatcttcc tcatgtgtat atttttttaa
      241 ttatacacta acttataaga gcatgatata ctctttaagt gagaggtcaa aacatatcta
      301 tgaatatgta gaaagaaata ttgataaaaa tacctttact gaaattaatt ctaaagaaga
      361 ccaatcaaaa aagtcttata aaagttctaa aatcctttta gaatctgtaa aaaatactac
      421 tggagtcatg tatctatata ctgctaaaaa aacaaaagat ggatcactag tatatgttgt
      481 tgatggttta aattcatcta ggagtgattt tagaaatgct ggtgatttga tagaaaaaga
      541 gatttgggga gaccttaaca aagcattaga taatcatata ttgttaccta aaaagattaa
      601 atcaacatct tggggataca tatttatatc gtattttcct attcacaatg atgttggtaa
      661 ggttgttgga gtaataggaa ttgaatttga agcaaaacat caatacaata catttaaatt
      721 tataagtata gttacaccaa taatagcatt attaacttgt ctaatatcag cattgattgc
      781 agtgattcta tttaaaagaa tatctaatcc tacatatact gattttgcta atacagatta
      841 tttaacaggc ctaaaaaata gaaatgcttt tgaaatagat atgaataact taaacaattc
      901 aaatctaaag aatctaatat ctatcatatc aatagattta gacggtttaa aaaagattaa
      961 tgataaattt ggccatcaga caggagactt atatattaag caagcaagta aaattataca
     1021 atatgtaata aataaaaaat atatagttta tcgtattgga ggtgatgaat acataatta
//
LOCUS       DAYAOX010000390         1077 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.396, whole genome shotgun sequence.
ACCESSION   DAYAOX010000390 DAYAOX010000000
VERSION     DAYAOX010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.396"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(242..925)
                     /locus_tag="ACGI57_003688"
     CDS             complement(242..925)
                     /locus_tag="ACGI57_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFG9718732.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT          417 a          166 c           83 g          411 t
ORIGIN      
        1 agacatttta attaatttat acactgttta ctatacttga aatacttcta ttaatctata
       61 tattgcctac tatacttaag acattttaat taatttatac actgcttact atacttaaaa
      121 tacttctatt aacttataca ttgcttacta tattttaaat tctttaatta atctatacaa
      181 tatttgctat atataaagta tatcaaataa cagcttcatt tttttgtaaa tttacagaaa
      241 gttaattttc attaaatgaa tcatctttat tgataaacat aattccaaag accactgcca
      301 aaataaaaaa acatattagt attgattttt ccacgaaaat ttttgtaaaa aaagttccta
      361 aaatagctcc agatataaag aatataataa tagcataata ctgtaaactc ttttttatca
      421 taaaaatatt cttagtatgt atataatgaa ataaagtttc tgtagcactt cttaaatttc
      481 cagtacacat tgtcgttgca aaactattcc catttacttt tctaaaactc tctacttgca
      541 ttgaacaaat aaaagaaata gcaacattta caaacatatt aaatcttcct ctaggaataa
      601 atgaaacaat aagtattgta atcatttcaa ttataatgat aacctgacgc caatgaatct
      661 ttgtcttatt atttgtaaaa tactttctta tcttctcggc tataaataca cctaacataa
      721 atgccaaaat aggaatcaaa taataaaatg attcctttac attaccttct gctatattaa
      781 gcccaaataa aactatattt ccagtttgtg cattagcaaa gactttacct cttaccacat
      841 aagtataaat atccaaaaat cctcctgtta ttgccaacaa agaacataaa aagaatgatt
      901 cagacatctg actcttgctt gtcatatata aacctctcgc tttctaatga tagatacaaa
      961 atcaaaatca ttttagattc tatttttcat tgatataaaa aaattattta tatttaaata
     1021 ttgtctatat ttatacatta tttgtattta tatagcattc atatttattt atattta
//
LOCUS       DAYAOX010000391         1054 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.397, whole genome shotgun sequence.
ACCESSION   DAYAOX010000391 DAYAOX010000000
VERSION     DAYAOX010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.397"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            87..434
                     /locus_tag="ACGI57_003689"
     CDS             87..434
                     /locus_tag="ACGI57_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFG9718733.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(584..>1054)
                     /locus_tag="ACGI57_003690"
     CDS             complement(584..>1054)
                     /locus_tag="ACGI57_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9718734.1"
                     /translation="AFAGGEGDFTTAFEPTATAMEKEGSGYIVTSIGKDSGEIPFTAY
                     STTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKAMQPFFEDISLEDLTTVVDR
                     YKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKDIVNTEFANNAIKNIK"
BASE COUNT          363 a          154 c          153 g          384 t
ORIGIN      
        1 tattcctatt gtgtaaagtg agaatactat tttcttatat tggtataata atagtaaatc
       61 taatatagaa aagaggtatt tctagtatgc atgaaaataa aaaaaatcta ttagacggaa
      121 gcttagctca atataatgat acagcagttc caaaaatacc agtatttgct ggtaacgata
      181 taacttcaga agtttattat tttaaaccta atcaagttct taatgctcat agacacccta
      241 atggtgaaca aatatttgtg ttcctaaaag gagaaggtaa aatgaaactt ggtgagcatg
      301 aatgtgatgt aaaaaacggc gatactgtat ttgtaccaac aggagagtgg catgaaatta
      361 ctaatggaag caatgaagaa atggtagccg ttcaaattac aaaaattaat gctggagcag
      421 aatacagagg atagatatat aagtacagtt agtactttaa tatctatttt attatataaa
      481 ctttaatctc aaatataaaa agagtctttt aaaaggctct ttttatattt ttgttaattt
      541 tcattataaa aacaagaaaa tatctagcta aaactaatat attttattta atatttttta
      601 tagcattgtt tgcaaactca gtatttacga tatctttata aggtgcttcc ttatctagtt
      661 ctttagcttc tttcataaca gtcatcaatt tcttcaaacc ttcctctttt aatacaggat
      721 tttgagacca tgcatctatt gatttatatc tatctacaac tgttgttaaa tcttctaaag
      781 atatatcttc aaagaaaggt tgcatagcct tagctacttc tttgctagat gcttgttgaa
      841 tccataattg acctttataa acagcatttg taaatctttg aattaaatct ttattgtttt
      901 tcatgtatga tctcgtagta gagtatgctg taaaaggtat ttctccagaa tcttttccta
      961 tagatgtaac tatatatcca ctaccttctt tttccattgc tgttgctgta ggttcaaatg
     1021 ctgttgtaaa gtcaccctct ccacctgcaa aagc
//
LOCUS       DAYAOX010000392         1034 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.398, whole genome shotgun sequence.
ACCESSION   DAYAOX010000392 DAYAOX010000000
VERSION     DAYAOX010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.398"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(112..405)
                     /locus_tag="ACGI57_003691"
     CDS             complement(112..405)
                     /locus_tag="ACGI57_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HFG9718735.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(411..626)
                     /locus_tag="ACGI57_003692"
     CDS             complement(411..626)
                     /locus_tag="ACGI57_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718736.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT          341 a          127 c          127 g          439 t
ORIGIN      
        1 tttatgttaa aaatagatta tttagaagaa ggggaaatct taaaaaaatt tgaatatccc
       61 ctttttatta tttaattaaa atgaaatagt tagaaatata ttttatttag tttagtcaat
      121 aacattttgg gattctttat aactacatgt taactgttga ataggtttat catatggttt
      181 aacccatcct ttattaatac ttaattctgt aagtgctgtg tgaccttcta ccatttgagt
      241 taatgaagct gaatatatag ctcttagttc aggtgtacta cttgtcaatg cagaattaag
      301 atacatattg gcagatgctt tagcagatga aagcatactt aacattatta ctttatcgtc
      361 tatataagta ttgttcttaa ttagattttc tatcatattt ctcatgttat ctatacctcc
      421 tcagaaatta gtatttcatt ttcaactata aattgttgta tagctttaat tcttccttct
      481 gttgctaata tactagattc tgattgtctt tttagatctt catctgatat taacatattt
      541 attgctcttt gcataagtaa tccgtcagac tccattttta taattgctgc taaagatatt
      601 ttttcagttt ctgaaagtat tctcatttaa tcatctccta tattgtaatt tatgctttaa
      661 tagtatgtct ttcttttcaa ggaaataacc attacacttt tttacagtat tttatattct
      721 cgttatatca aaatttagtc gacttaatat attaatataa ttttgtagga tatttatcat
      781 taaatatcta taaaataaat tgtttgaata ttcataaagc aaaatttatt tgtatgtact
      841 tcttcaagag taaatatgag ttttttcaaa aaattaattt ttatgataat tattaagggc
      901 tatatttttc ttaaagttgt gattatcatt attaatttac aataaaattg ccgaaaagcc
      961 ctctccttta ggtgtaggga tgaaaggtaa ggtcttaata taaaaaagtt agaataatgc
     1021 ttgtaatcta atac
//
LOCUS       DAYAOX010000393         1033 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.399, whole genome shotgun sequence.
ACCESSION   DAYAOX010000393 DAYAOX010000000
VERSION     DAYAOX010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.399"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            288..968
                     /locus_tag="ACGI57_003693"
     CDS             288..968
                     /locus_tag="ACGI57_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9718737.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          452 a           64 c          167 g          350 t
ORIGIN      
        1 atagctttga attatataat tgtatatgct tatctttata cagtaaataa aaaatcaaat
       61 gaaataccaa ataaagtgta tatcttaaaa taacttacta gagatacaag gatgctatgt
      121 gaattttgaa gtgtgcagtt tagataataa atatatataa ataataaaga aaatggattt
      181 atagtattta aaatttctta taagggtaaa aaatgctatt agaaaatgta ctaaaaatat
      241 ttttccaatt ctttatagaa aaataatgat atttaggaga gattgtaatg caaattgatg
      301 tattgataat tgatggagat aagaatagtt ctcaagagct gaaaaatttt ttagaaggaa
      361 aggggataaa tgtctattta gcatataatt gtaaagatgc tattataaaa atatttagca
      421 agaaatttga cttaatattt ttagagataa ttttagttga tggtgatggt tgggattttt
      481 gtaaaaaaat tagaaatata actacttgcc caattattca tataacttat ataaatgaaa
      541 atcaaagtat tttaaatgct ttaaattctg gaggagatga ttatttaata aagcctttaa
      601 atttagaaat attatatgcg aaagttaaag ctattttaag aagaagagat ttatttttaa
      661 ataataataa aaataatata aaactagaag attataatag agctagtgga gtaataaagt
      721 tagaaaataa aatgataaag ttaactccaa cagaaaatag attattaaat tactttatag
      781 agaatgctga aaagactcta actattaagg agatatatga acatgtatgg atgaatgagt
      841 atttagatga taattatagt gtagtggttg ctgtgagtgg attaagaaag aaaatagaga
      901 aagattataa aaatcctcaa aaaattataa cgataagaga agtagggtat tactttaaca
      961 agatataaag ctttataaac agaatgaatg ataaggcata gataatatgt tagtatatta
     1021 taagagtttt tgt
//
LOCUS       DAYAOX010000394         1028 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.400, whole genome shotgun sequence.
ACCESSION   DAYAOX010000394 DAYAOX010000000
VERSION     DAYAOX010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.400"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(60..950)
                     /locus_tag="ACGI57_003694"
     CDS             complement(60..950)
                     /locus_tag="ACGI57_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9718738.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
BASE COUNT          344 a          174 c          104 g          406 t
ORIGIN      
        1 ttttcaaata acagtcatac taaaacaatt tcatcatata atttgaaaat tattactatt
       61 tacacagctt ttatcatcat cttaccacca tcaaatatat attcaaccat attattctca
      121 tataaataag ttatataaga acgtattgta cttccaacca acacatattg attagcatca
      181 agcttgacat tgtacaaatc acacattttt tcaataactt catcaatcat aactggctca
      241 taacaaacct ttttaatatt atctattatt tcttccatct tcttcttatt aatattaatt
      301 aaacttttta tatctgtagt tttgacagag tgacttggaa caaagaaatc agcctctaac
      361 attctcaatt tttctagtgt cttaaactgt gaatctatat ccagtaggaa gaaaaaatga
      421 tatttagtaa ttatattctc tggtgtaaga ctatctgcaa caaacaaaac attatctgga
      481 gttctaacac caatcatttc aaaataatga cctggaagag aaatcgcttc aagttctgta
      541 tctacaattt tgccatttga agggataata taatcaactt cactttcctt tgctaataaa
      601 aatttattct tcagtgcttt acttggatag ccaccatata aaaatgctga ttctaatatt
      661 ggattttctg taaaatatcc ttcaatttta gtcgtagcaa tcttgcattt tgtttgattc
      721 ttcaaatatg catttcctcc tatatggtct gcatttgaat gagtgtttat tattaattta
      781 ggatttaaat tatgttcttc taataatctt aaaacttgtc tagctgtatc cttattattt
      841 ccactgtcaa ttaaaattgc atccttacca tctacgtata cacctactat tgtagggagt
      901 ggaatataat atgtatttcc ttttatatgt tctagagtta atttagccat tttataacct
      961 ccctttaaat ttacttttaa ttatagcatt aattttctga aaattccaat caaattgttt
     1021 ttataata
//
LOCUS       DAYAOX010000395          985 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.401, whole genome shotgun sequence.
ACCESSION   DAYAOX010000395 DAYAOX010000000
VERSION     DAYAOX010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.401"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            158..664
                     /locus_tag="ACGI57_003695"
     CDS             158..664
                     /locus_tag="ACGI57_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HFG9718739.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          393 a           89 c          155 g          348 t
ORIGIN      
        1 aatatgaaaa ctacttattg cgaaagtaat tgatttgttg tattataatt tacatgaatt
       61 atgtaggaca atcaaaagtc agtattataa gagtacatat attcaaattt ttaacaatta
      121 atttatataa ttaaaaaatg aagggtagga tgaagtcatg ggttttagag aagtaaaaat
      181 tgaggaatta cagtttaatc catttactaa gattggcaaa gaatggttac ttataacagc
      241 tggtgattca gaaaagttta acacaatgac tgctagctgg ggtggtgttg gagtctattg
      301 gggtaaaaat gttgttacaa cttacataag accacagaga tatacaaaag aatttgtaga
      361 tagtaatgat acatttacag ttgccttctt tgatgaaacg tatagagagg ctttaaatat
      421 ttgtggtact atttctggta gagatataaa taaaatagaa aaagcaggac ttacaccata
      481 ttttgtggat gatacagtag cttttgaaga agctaatatg attatagtat gtaaaaagct
      541 atatcatggt aatatgccac cagaaaactt tgatgctaaa gaaaatgata aaaaatggta
      601 tccagagaaa gactaccata ctatgtacat ttcagaaatt ataaaagttc ttgtaaaaga
      661 ataattataa taaaaaatat atgaaaagaa cttattataa gtaaatgact gtgctttctt
      721 atctgatatc taagtagatg ctagtaacta aataaatatt aattttaaat aaatactatt
      781 ataaaaaaga tgaactatat ttactatagt tcatcttttt atttatataa gcacttatat
      841 aagtagagat ttatttgaat aaaagtattt atttgaaata tttgagtata ctttataagt
      901 atttttatgc taactagaaa cacttaattt atggcatttg tatttgattt attttaatgt
      961 aaataatttc aaagttaatt ttatt
//
LOCUS       DAYAOX010000396          979 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.402, whole genome shotgun sequence.
ACCESSION   DAYAOX010000396 DAYAOX010000000
VERSION     DAYAOX010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.402"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            308..712
                     /locus_tag="ACGI57_003696"
     CDS             308..712
                     /locus_tag="ACGI57_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFG9718740.1"
                     /translation="MDKTLKKDLNQEYTESKSLIMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            729..>979
                     /locus_tag="ACGI57_003697"
     CDS             729..>979
                     /locus_tag="ACGI57_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718741.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIY"
BASE COUNT          413 a           82 c          145 g          339 t
ORIGIN      
        1 tatgtcaact gattttagaa aaaactaatt accaatatta tacatttaat aaatgtaatg
       61 tattttatga aagtctgcca tattaaatag atttaaattt ttttaaaaaa tttataaata
      121 ttgttcaaaa atcctgccaa taaattggaa atactaaatt aaaaaaataa taaagtttag
      181 cttatagcta aataaaagtt tacttataag gtgaactttt tgcattttca gttttgacat
      241 acatattttt ttgtaatata tttatattat caattagaga taaggagagt gagaagagat
      301 atttaatttg gataagacat taaaaaagga tttaaatcag gaatatacag agagcaaaag
      361 tttaattatg aataaaagta agtatatgaa tgtttgtttt aagaattata ttaaagtagt
      421 agaaggaatg tatactaaga gggatttaat tccattaaaa ttaataaact ctatggataa
      481 aaataataag attagaaaaa cattatatga attatcttgt gaatttgaat atccaaaaac
      541 ttctttatct tcattgttta cggagctgaa aaaaatggat tttttacaaa gagtgaaaaa
      601 tggtgagtat atgataaatc cacatatagc atataaaggt agtagagctg ataaagaaag
      661 tttattagca gaatataatc agataaaaag acctaataag gttagtaaat aaggaagaag
      721 gtttttatat gagtttaaaa cttgatagaa atgttctaca gtggtttgac tatgtatttg
      781 agaatgaaaa aactagtttg agacactata attttaattg tacactaaag gagattagtt
      841 ctaccagttt gaacaaagta gcttttattt tagaaaaaaa taactctaag tactggaagc
      901 tttattttga aataccagct gaagttactt taaaacttaa gcaaaacatt catcctttat
      961 ttagggaata tatatacga
//
LOCUS       DAYAOX010000397          978 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.403, whole genome shotgun sequence.
ACCESSION   DAYAOX010000397 DAYAOX010000000
VERSION     DAYAOX010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.403"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            340..807
                     /locus_tag="ACGI57_003698"
     CDS             340..807
                     /locus_tag="ACGI57_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9718742.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          373 a           66 c          164 g          375 t
ORIGIN      
        1 aaatgcatat acttgtaata tttaaaggtg atattaatat tattagtgta tattaatatt
       61 cccttgaaat taaaaaaaca tagatatttc agtaatctaa aatagtctac aaaaataaca
      121 gttttagcaa aattaataag aatacatata gatttaaacc taaaagtatg aacatgtctg
      181 ttttttacaa gattaaaatt tatattatat gataaaatta acttaactat ttcaataaga
      241 cttataattt ttttctttat actagacctt attgaaacct ttatagttta attctaatta
      301 tatattataa ataaagtatg tgtatattaa ggaggtaata tgaagttttt aaatgtttta
      361 gtagtagttg aagatattga gaaatcaaaa aagttttatt atgatgtttt ggggttaaag
      421 gtaatctgtg attttggaga aaatgttgta ttagaaggta atatatcatt acaagaaaaa
      481 aagctttggt tagaatttat taataagagt gacagtgaag ttaagttcaa tggaaatgat
      541 gctgagttat attttgagga agataatttt gatacttttg ttgaaagatt gagtacaatg
      601 aaagatatag attatgttca tctagctata gagcataggt ggggtcaaag agcaattaga
      661 ttttatgatt tagatgggca tattattgag gttggagaaa ctatgtcatc tgtgtgtaga
      721 cgttttttag atagtggtct ttcaatagat gaggtagcta aaagaatgga tgtaactgtc
      781 gaatatattg aatcagtttt agaatagatg gattatatta aagtaaaatt gaggtgaagg
      841 ctgtaagtaa acttgtgtga aaacaaattt gcttacagtt tttatttttc taaaataaag
      901 aatataatac ttgtttagct ttatttgagt ttttaaaaat attatgatat ttcttatata
      961 tttatactaa agtttaaa
//
LOCUS       DAYAOX010000398          949 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.404, whole genome shotgun sequence.
ACCESSION   DAYAOX010000398 DAYAOX010000000
VERSION     DAYAOX010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.404"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            310..>949
                     /locus_tag="ACGI57_003699"
     CDS             310..>949
                     /locus_tag="ACGI57_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFG9718743.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISIFVLIHIILKI"
BASE COUNT          344 a           76 c          108 g          421 t
ORIGIN      
        1 atattcatta atctaaagta ttatttctat ttaaccatat caaaatatat tttactattt
       61 ttaattatta caatataaac ctgtcttttg ttattatagg taaactaaaa gtaagaaatg
      121 atttttatat ttttaaactt tattattggt aacttatctg taaatatttt aaaatgtact
      181 atagaaagtt gttaaaaata ttaaataatt atattaaatt taagtgtgtt ttttatgcta
      241 taattagtat gtatttttta tgatatattt tataaaaaat ataatttaat catttttgag
      301 gatattatta tgttagatgc atttataaca attgtttctt ctattataga aatatggtca
      361 tgtaaaaaag tttttgatta tactagtcag tcaaagaccg acttaaaaaa actaaatatg
      421 tattttctct tttctgtttt tctcattatt tcttctttat atataaatat aggagctaat
      481 agtagaatct tgatatgtat atttgtaatg ttcgttttct ataagtctaa ctataaggtt
      541 aatatatata aatgtctggt aatctcattt ttttattggt taatattaat aggtataaat
      601 ggattaagta tgttattagt agcaacagta agcaatttgg ataatatgaa tgttttactt
      661 agtggcagtt tatatagata tgaagcaata ttcttgggaa agataatgtt aggtatatta
      721 ctttatgttt ataaggcttc ttttaaaaat tttaaaataa gtaaaaaaga gatatacata
      781 tgtattccta tgatagcaaa tttattgtca ttttttgtac tttatgatta tattattaat
      841 cttgtaaaaa tctctcctga aagaagaact gcttttttag taatagtttt actattaata
      901 ttatctaaca tttcaatctt tgtattaata catataatac ttaagatta
//
LOCUS       DAYAOX010000399          945 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.405, whole genome shotgun sequence.
ACCESSION   DAYAOX010000399 DAYAOX010000000
VERSION     DAYAOX010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.405"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(258..647)
                     /locus_tag="ACGI57_003700"
     CDS             complement(258..647)
                     /locus_tag="ACGI57_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9718744.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
BASE COUNT          322 a          144 c           81 g          398 t
ORIGIN      
        1 tatctgtcaa ctatttcttc taaaattata ttccttttat aatttcaata tctttatcat
       61 atttacatga tttaagctat tgtagtattt catatatctt taaattttaa ttggtattat
      121 tctctatatt ttttactatc aaacactaag gaaataatca cttttcttta tataatgttc
      181 taccatcaca taaaactaac attaaatcca aacgctactc tcaattgtct caaactaaaa
      241 agatactgat agctttatta gtcatcaata tcttcttctt tcaaattttt aaagtattct
      301 tctaatttgt ccaagctctt actatctaaa acttcatcat catgtagtgc tgaaataaaa
      361 ctcttaaatg aattgttatg cataaaacta aaaaaactct ttgtctcaac ttttaagtaa
      421 tctttctctt ttatagctat agtataatgc gtacatctat ctaccttatc agtagttaaa
      481 aagcccttat ctactaaatt ttttaaaaat gtcaatgttg tgctcttttt ccaatcgtat
      541 ttcaacttca tgtcttctat gatatctctt gaggctaata catcatctac tccccaaata
      601 tacttcatta gttttagttc tgcatgtggt aatttattaa tcttcattag tttacacctc
      661 ttaccaattt atgtataact aaactttata acaataagtg tatgtaatca attagattgg
      721 aataaatata aattccaatt tattctatat aaaaataatt aataaaaaag aagtactcta
      781 ctttagagag tacttttgga ttatatattc aaaaatttat ctaacatgat tagagtatgt
      841 tttatgcttt agtgtgataa ttttagttcg ttttattatt gcaacatgta ttatatttca
      901 ttataatcta taagcttaaa tacaggatat ttatttcata tactt
//
LOCUS       DAYAOX010000400          934 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.406, whole genome shotgun sequence.
ACCESSION   DAYAOX010000400 DAYAOX010000000
VERSION     DAYAOX010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.406"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..566)
                     /locus_tag="ACGI57_003701"
     CDS             complement(<1..566)
                     /locus_tag="ACGI57_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718745.1"
                     /translation="MSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDLNNTYDV
                     LLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTDFNSAYG
                     FGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNVERFIYS
                     QFSLIFFIVIANKMKGKNSYLVELAAIPL"
BASE COUNT          372 a          115 c           94 g          353 t
ORIGIN      
        1 agaggaatag ctgctaattc taccaaataa gaattttttc ctttcatctt atttgcaata
       61 actatgaaaa atattaatga aaattggcta tatataaatc tctctacatt taaagtcata
      121 aataactgtg gaaaagaaaa tatcattatc atagtaagta aaattaaagt taatctctgc
      181 atttttatct ttttaatatg attaaagact aaagttattg acatagcact agttattatt
      241 tgtagacaaa ttagtccaaa cccataggca ctattaaaat ctgttgtccc aggatttgct
      301 attgaagtac aaaataggta tattggatat gcaaaaattg atataattgc tgaaaacaat
      361 atatgcttac tattgtcttg acttatagca aatacattta aattgaataa aactcctgta
      421 tctgtatcta acaatacatc ataagtattg tttaaatcat aaaatatagt agtcttagaa
      481 aaaaatatct tagttattat taagtaagtt attacaacta atataaacaa aaacatctcg
      541 tttttatatt ttaatatttt actcatcaat atatcaccat ccattcatta tataggattt
      601 gctgacacct acaatttatt ccttatattt aatagccatc ttcataatgt tcttacttgt
      661 tttattgtaa ttgaataact ctttatttac aaccacaata tttactttat tgatgttata
      721 atcagtcaag tattgtttat ataacataga ttagtgttgt ataaaatgag aatttgtaag
      781 caatattaat gtaaaatatt tgaaaaactt tatataaaaa tatacaatgt tacttatgga
      841 attaaactat aacaaattaa agcataattt aaggagacaa aattataatc tgtctcctta
      901 aatattaaaa ctatatttta aaacattact aaat
//
LOCUS       DAYAOX010000401          933 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.407, whole genome shotgun sequence.
ACCESSION   DAYAOX010000401 DAYAOX010000000
VERSION     DAYAOX010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.407"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            103..666
                     /locus_tag="ACGI57_003702"
     CDS             103..666
                     /locus_tag="ACGI57_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718746.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
BASE COUNT          377 a           92 c          137 g          327 t
ORIGIN      
        1 taaaatcaaa atttgtaaaa ccacatttat tatgttagaa tatcttatga tattaaattg
       61 taagtgtata atattattta gttactagat tggggggaaa tgatgaataa agtctatatt
      121 gtaacaacat atacaggaac tatattatct tacttaataa ggaatataag taaaaagctg
      181 tatacacatg tgtcaatatc attaaatgaa aatctaaaac caatgtattc ttttggaagg
      241 ctaaatccaa ggaatccatt tattggagga tttgttgaag aaaatataaa tcaaggatta
      301 tatgctataa gaaaaaacac tgtgtgtagg gtatattcgt tagaagtaga taatttacaa
      361 tatgaaaatc tatataaaaa tataaaatta attagtgatt atagagaaga ttactattac
      421 gatacaatgg cattaatata tttactaatg aatattcaga gacaatctga atataaatat
      481 gtatgttcaa attttgttgc tgacatgtta gaaaaaagtg agatagacat tttaaataaa
      541 caagcatttg aagtaacgcc aaatgatttt tataatttat ctggcttaac cttagagtat
      601 gaaggtttat tatcagaata taattctaga caacattctt atgtatcaaa aatcgcaaac
      661 atttaaaaac ttgatttaga gtttttcata taaaaaaata ttgctagata attaggctga
      721 agatagtagt ttaacttcga taggattaag aatttgtaaa tcttagttag aatactttct
      781 tcagttttta aatttctgct aatatttgta tttttataac atttttggat atatataata
      841 cagaatagtg caaaaatccc actcctttag gtgcgggatg gatagcacaa acagtgtata
      901 aaaagtattt atacatacac gtataatgtt aaa
//
LOCUS       DAYAOX010000402          926 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.408, whole genome shotgun sequence.
ACCESSION   DAYAOX010000402 DAYAOX010000000
VERSION     DAYAOX010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.408"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..868
                     /locus_tag="ACGI57_003703"
     CDS             <1..868
                     /locus_tag="ACGI57_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9718747.1"
                     /translation="GVMLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGIT
                     GLGVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANM
                     ATLDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMG
                     IVDCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDIS
                     KKIMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDI
                     HEALGNGFKKIE"
BASE COUNT          333 a          104 c          149 g          340 t
ORIGIN      
        1 aggagtgatg ttattgaaaa aagatagaat aactttttca gaaattttaa tattaattgt
       61 aggctgtata ttaatggcca tttctcttaa cttatttttt aatccacatg caatcgctgc
      121 tggtggtata acaggacttg gagttgtttt aaattcatta ttcggtgtag aactttggat
      181 tgtcaattta cttttaaatg ttccattatt catatttgct tacaaaatac tttcaaaaaa
      241 agattgtttt aaaacggttt taggaataat ttttcttacc atagcactta agcttactgc
      301 aaatatggct acattagata taacaaatga tatgtatctt gcaattatat ctggttctat
      361 tttaatggga gttggtcaag gacttatttt tagaataaat ggctctacag gtggaactga
      421 tttaatggca ctcctactaa ataaatattt tcctacattt agtatacctg ttttaatggg
      481 tattgtagac tgcgttgtag ttgttttatc aggtatagta aatagacagg ttgaaattgc
      541 actttattca actgtggctt tatacatttt agtaaaagtt agtgacttgc ttattgaagg
      601 atttaattat tccaaatcat tcactattat atctgacctt tcaaaagata taagtaaaaa
      661 aataatggaa gatttagata gaggtgcaac tattctaaaa ggagaaggtg catatactgg
      721 agaaaataaa aatgtacttt tagtagtagt tgaaaagaaa gaagttgtcg aattgaaaaa
      781 attagtaaaa aatgtcgacc ctaatgcttt tattataatt acagatatac atgaagcttt
      841 aggtaatgga tttaaaaaaa tagaataact aacaaataaa tagcaagtat aattaacata
      901 tacaaattca actttttata tgttat
//
LOCUS       DAYAOX010000403          925 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.409, whole genome shotgun sequence.
ACCESSION   DAYAOX010000403 DAYAOX010000000
VERSION     DAYAOX010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.409"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            69..>925
                     /locus_tag="ACGI57_003704"
     CDS             69..>925
                     /locus_tag="ACGI57_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFG9718748.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILI"
BASE COUNT          374 a           80 c          131 g          340 t
ORIGIN      
        1 gtttagataa caatttatct tgtttaaaaa ttttctattt gaaaataatc taaataatat
       61 acttttatat gggagtgatt ataattcaaa taagaagacc attattaatt atattaatgt
      121 ttgtggtagc tatatcattt atcattacaa ataacacaaa aaacgacgaa gaacttaatg
      181 ataagataat aactatacaa ggagttgtca aaggaaaaat tgaaaagaaa agatacaatc
      241 agtacaaggt aggggttttt ctaataaatg attatacaaa aaataaaaat ttgaagattg
      301 gtcagaaagt taatattaca ggtaaattca aaagtttaga taaaatgaag tatgatgatt
      361 ttgattatgg aaggtatata aaaagtactg gatataaagg tatagtgtat ataaataact
      421 ataaaattat tgataaaaat aaactttact ctttaatagg ggaaataaag ttttatatta
      481 gtaaaactta taggtactta tataagaaaa actcagattt tataaattct atactattag
      541 cagaagttga aaatttgact gaggaacaaa aagaaatttt tactagaact ggaactagtc
      601 atgtaatatc tatatcaggt cttcatacag gaatattatg tgtaattata tcctttctat
      661 taagaggtat aaataaattg tataaattat taatactgtc gatttttata actttatatt
      721 gtataatggt aggtgcgtct ccatccataa ttaggtctat agcatttgtt atgatttttt
      781 atttatcaat atttattgat agaaagaaag atggaatatc ggcattgtct ctaattggga
      841 taattttaat tatgaataac ccctatgtta tatataatgt aagttttcaa ttgtcttttt
      901 tagctacact ttctatatta atatt
//
LOCUS       DAYAOX010000404          919 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.410, whole genome shotgun sequence.
ACCESSION   DAYAOX010000404 DAYAOX010000000
VERSION     DAYAOX010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.410"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            231..383
                     /locus_tag="ACGI57_003705"
     CDS             231..383
                     /locus_tag="ACGI57_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718749.1"
                     /translation="MKNLNKNTILGEVIITITACDDEKEIQIVIKNQVDNILKNKNKE
                     YRIKKI"
     gene            622..>919
                     /locus_tag="ACGI57_003706"
     CDS             622..>919
                     /locus_tag="ACGI57_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9718750.1"
                     /translation="MKKIKINLTHTKSNLAKKYIEVQKKNMLIHTKNKTLDAICILEK
                     IEKDLNIEELVRCHKSYITNLNYVENIKSNTAILDSQEEVPISRYRYKDIKEGFL"
BASE COUNT          460 a          112 c          102 g          245 t
ORIGIN      
        1 caaaaataaa caataaaaaa tatccgaaaa aatcagttta acatcataac taaatagcta
       61 tatacctaca cttgcaaaaa acaacaaaac aatcaaataa ccacttatag actcccctcg
      121 tctccaccat tgaaatccac gaatacctat atccaattgg atataggtat ttttatacat
      181 ttttaattta aatattattg ataaatacat ttaaaaagtt ttaacctaaa gtgaagaatc
      241 taaataaaaa tacaatatta ggagaggtta tcattacaat aacagcttgt gatgatgaaa
      301 aagaaataca aatcgtaata aaaaatcaag ttgataatat attaaaaaat aaaaacaaag
      361 aatatcgaat taaaaaaata tagctcaaaa gaagaatcat tacaaagtta attgagagga
      421 tacactactc ttagatataa aaatggcaga actaaatagt atagatatcg caagaaacat
      481 aagaaaagta gataataata tttataacat ctctaataaa ttatgtacag gaaagatatg
      541 aagtaagagc ttattgatac ttattaaagc caataaattt tcaagaacta gaaaaacaca
      601 tattaacatg taccaaaaaa attgaaaaaa ataaaaataa atctaacaca tacaaaatct
      661 aatctagcaa aaaaatatat agaagttcaa aaaaagaaca tgctaataca tacaaaaaat
      721 aaaactcttg atgctatatg tatcctagaa aaaatagaaa aagatttaaa tatagaggaa
      781 cttgtaagat gtcataaaag ttatataact aacttaaact atgtagaaaa tataaagtca
      841 aatactgcaa tattagacag tcaagaagaa gtacctatta gtagatatag atataaagat
      901 ataaaagagg ggtttctaa
//
LOCUS       DAYAOX010000405          915 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.411, whole genome shotgun sequence.
ACCESSION   DAYAOX010000405 DAYAOX010000000
VERSION     DAYAOX010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.411"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            191..841
                     /locus_tag="ACGI57_003707"
     CDS             191..841
                     /locus_tag="ACGI57_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9718751.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
BASE COUNT          394 a           91 c          158 g          272 t
ORIGIN      
        1 tacaataact tgatttgcaa tacaatgaat taatctatag tataagagca tgatttacag
       61 tacaataatt taaattgtag tacaaaagta ctatcaaata agaatttcta ctttaaaaat
      121 attactaaaa gtaattaaag tccagtaatt caatataata tggcaaaaga atagaaataa
      181 ggtgagagta gtgaaagtaa agtcaaattt agatgaaatt ttatatcata agattattga
      241 aagtcttatt cgtggtgaat actctgtagg tcaaaaaata ttactaaatg acctttgtga
      301 aaaatttgaa gtaagcagaa caccagttgt acaagcagta aaaatgctaa ataaagatgg
      361 agtgcttact attatgacaa atggtaaagt gtacgtacca gagtatgaat atgatatggt
      421 aaaacaagtt tgtgaaacga gaacactgat tgaaacatat gctttagaaa aaatgatgca
      481 agaagaggaa gaggtttttc aacaaaagct agatgtaata aaaagatact ctgataaatg
      541 cgaggagtac tatcgtcaag aaaaatctgt agaacttgca ctagcagatt tggaattaca
      601 taaagccatt gtagaagggg caaataatga gattttaaat gatgtatatg ttggtataca
      661 aggaagattt atagtagtga actatttaat tcgaccatta aaaaatcgta attatgaagg
      721 aacagtacaa gaccattttg aaatattaaa attcattgaa gacagagata caaaaaaagc
      781 agtagataaa ctaagaaatc atattcaagg aactattaaa agattctgtg aagatgaata
      841 ataagttttg aagttactat atatacttat ctattaagtt gctaaatgta gtctaagtat
      901 aatctaatta tagtt
//
LOCUS       DAYAOX010000406          896 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.412, whole genome shotgun sequence.
ACCESSION   DAYAOX010000406 DAYAOX010000000
VERSION     DAYAOX010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.412"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..714
                     /gene="cwlD"
                     /locus_tag="ACGI57_003708"
     CDS             <1..714
                     /gene="cwlD"
                     /locus_tag="ACGI57_003708"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HFG9718752.1"
                     /translation="GDVVRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNK
                     TIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYK
                     EENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSK
                     ELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGFLSNEKECKLLTDE
                     TYQEKIAWAIYIGIQKYLS"
BASE COUNT          393 a           99 c          144 g          260 t
ORIGIN      
        1 ggtgatgttg tgagaaagta cataaaacat ataattttta gttttgcaat gatatgtcta
       61 gtagtagtat ccatatttga aattaaaaat atttctgaag atgttatcaa gtatatgcca
      121 gtaacaaaca aaactataat tttagatgct ggtcatggag gcattgatcc aggtgcatta
      181 aataaggata agagtacatc tgaaaaggat attaacctag caataacact taagcttaga
      241 gagcttatag aatcaagtgg tggccttgta atattaactc gagaagatga cagtagcctt
      301 tataaagaag aaaataataa aactacaaga caaaaatata atgagaattt gaaaaataga
      361 aaagaaataa tatctaattc taatgccaat atgtttgtct ctatacatct taatgcattt
      421 gaacaatcaa aatactatgg agctcaaact ttttatccta aagacaaaca agacagtaaa
      481 gaattatcga agtgtattca agaagaactt aaaagagtag tagataagac aaacaacaga
      541 gaagtcaaac caagggatga tatatatctt ttgaaagaaa ataatattcc atcagtactg
      601 atagaatgtg gttttttatc aaatgaaaaa gagtgtaaac tcttaactga tgaaacatat
      661 caagaaaaaa tagcatgggc aatctacata ggaatacaaa aatatttaag ttgaaataac
      721 tataaaaata ggtctaaata tgtaaatagc ttgtattaaa gcagttaaaa tgcattaata
      781 taggctattt ttattttgac aaaaaaatat ttaaaataaa agttaaaaag ttgttgactt
      841 agaataatat agatgatatt atatatgagt gcccaaaagg agcaccaaaa taagac
//
LOCUS       DAYAOX010000407          888 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.413, whole genome shotgun sequence.
ACCESSION   DAYAOX010000407 DAYAOX010000000
VERSION     DAYAOX010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.413"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            77..811
                     /locus_tag="ACGI57_003709"
     CDS             77..811
                     /locus_tag="ACGI57_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9718753.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
BASE COUNT          368 a           95 c          145 g          280 t
ORIGIN      
        1 ctagaggaaa atctacattt tctcaatttc tttaattaaa ttgacctcta attaagtaat
       61 tataaggaga aaaattatga atttagcaaa gggattagat gttttaaaaa tttcatcaaa
      121 tgtattcggt gaagataaag ttatgtatat tcctgtaatc tacacagaag atgatgcaac
      181 acttattgac acaggtcttc cgggtcaagg tgatttaata attgatgctc ttaataaaag
      241 taacacgagc tttgataagt taaaaaatat aataattact catcatgata tagaccatat
      301 aggaaacata aattatctaa gagaaaaatc taagaataat atcaaagtat atgcatacaa
      361 aagtgaaatc agctatataa caggagaaga aactcctttt aaactttata tgttggaaca
      421 aatggtagat aaaattgatg ataagatgct tagtatgcta aatgttatgg gacttggatt
      481 taaatcatca tatactaaag tagatgtgtc attagataac catgaaaaat tgaatctagg
      541 tgaggaaata gaagttatac atacaggagg tcacactaga ggtcacatat gtctttattt
      601 aaaagagtct aaagtactta ttgctggaga tttgttacag gtagaaaatg gagaacttaa
      661 gcctgttgat gtaatgcaca gcaacaaaca agagttaaaa gatgctatta aaaatataag
      721 taattatgat atagaaacga tagtttttag tcatggtgga ttatatcaaa gaaacataat
      781 tggaacattg aaaaacctaa ttattgagta gtactatatg atttacaata atttcttaaa
      841 ttacttaaaa ttatatctaa agatgtagaa tcaaaattaa atataagt
//
LOCUS       DAYAOX010000408          887 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.414, whole genome shotgun sequence.
ACCESSION   DAYAOX010000408 DAYAOX010000000
VERSION     DAYAOX010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.414"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            33..308
                     /locus_tag="ACGI57_003710"
     CDS             33..308
                     /locus_tag="ACGI57_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718754.1"
                     /translation="MTLLELYYRKAEINKIVWARIGFNLYLSKGVALTLTDAGIGISG
                     LLAGAIPGIGQYLPLIVRLITMYLESNITSGKIFKFKKYVIIKEVLV"
     gene            656..>887
                     /locus_tag="ACGI57_003711"
     CDS             656..>887
                     /locus_tag="ACGI57_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718755.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     GNIDRKRTIKLEVLYRYVTGFEYIAIGLFTKRL"
BASE COUNT          334 a           89 c          129 g          335 t
ORIGIN      
        1 taagctaatt aaaaacctta agaagaacga tattgacact acttgaatta tattatagaa
       61 aagctgaaat aaataaaata gtttgggcta gaataggttt taatttatat ctttctaaag
      121 gagttgcatt aactttaact gatgctggta tagggatttc aggacttttg gctggtgcta
      181 ttcctggtat aggacagtac ttgccactta tagttagatt aattacaatg taccttgagt
      241 caaatataac atctggaaaa atctttaaat ttaaaaaata cgttataata aaggaggtgc
      301 ttgtgtaacc aaatactact tacaatctgt agaaatcaat aggtgataaa atgattataa
      361 aatatctact ttttgcaatt gtttcagcat taattttatt attttgtaat aaagcattcc
      421 aactaagtaa attacacaag ttcattatat tgtttgtttc attttttata ctaacttttg
      481 tagcatcatt cattagtaga taaaaaataa gatggtgggg ttaacttacc atctttttct
      541 catttaatat ttgtaatatt ttagatactc tttatacttg tgttactgtt atactttgat
      601 taggaaaatt aatacaacat gagttgatga ttatattata aaggaggtat cttctatgaa
      661 ttttaggaac atttatctta tacttatggg attgggaatt ataatattaa ctacaataat
      721 atctttaatt gaaattaaaa ttggtttttg taatgaaaaa tattttaata aattagaatc
      781 aaaatatgga aatatagata gaaaaagaac tattaaacta gaagtacttt atcgatatgt
      841 aacaggtttt gaatatatag caataggatt atttacaaaa agattag
//
LOCUS       DAYAOX010000409          869 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.415, whole genome shotgun sequence.
ACCESSION   DAYAOX010000409 DAYAOX010000000
VERSION     DAYAOX010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.415"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..756)
                     /locus_tag="ACGI57_003712"
     CDS             complement(<1..756)
                     /locus_tag="ACGI57_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFG9718756.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISE"
BASE COUNT          267 a          166 c           83 g          353 t
ORIGIN      
        1 ttcagatata ctttttgcag tttctataac tcttttaatc ttatactcaa agtccccttc
       61 tctcattctt atagttggac ctgacaaact tatggctgca atagctttat tatttttatc
      121 taagataggt gctccaatac aagttagtcc atgttctagt tcttcatcat ctatagcata
      181 tccttgttct tttatttttg ctaaaaaaag catcatatca tcccaattat caattgtatg
      241 ttctgtatac ttgtgtattg gtgtttttct atatttttca aaatctatag aatcattaaa
      301 tgccataaga catttaccaa ctgctgaaca ataacattca ctacttgaac caacggatgg
      361 atttactgaa agtaattgat ttgaaccata ctctttatgt attatgatac ttcttggaga
      421 atcttgagct ctttcttcta atatagatac attaactact tcgttaaatt cttgatttaa
      481 ttttttcgta tatggtttta ctatttcagt caatgacatt ttttctccca caaccatacc
      541 tattgcatat aaatttattc ctagccaata cttaccattc tctgcatttt gtataacaaa
      601 acctttgttt tcaagagtaa ccaaagttct atgtacagta cttttatata ctcccatagc
      661 cttgcttatt tcagttattc ccatttctct cttttcatga tataacagca aaattatgtc
      721 tagggctcta tctaatgcat ttattatttc tcccatatac aaaaactcct ccttcaaata
      781 ttaagtgcta ctcccacact ctactaaata ttatatatta aaattacaaa attatctatt
      841 tatcttaatt atattttata aaacaaaaa
//
LOCUS       DAYAOX010000410          865 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.416, whole genome shotgun sequence.
ACCESSION   DAYAOX010000410 DAYAOX010000000
VERSION     DAYAOX010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.416"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..664
                     /locus_tag="ACGI57_003713"
     CDS             <1..664
                     /locus_tag="ACGI57_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718757.1"
                     /translation="KSYKLLNRFNLGLQNGEQQNINEIKFSIGSKLYLEIVNNKSKNG
                     YIEGHDSYSNFIIYDIEDNTFNISNKFMDNLNKEKNANPKEIGLLSKTKLDYMVKNNK
                     LNLIVTDMSNSITNLVYDINSNEIKLEDYEILDLKVDYNEASSGVRKVKLLDDKIYSI
                     SGIKNSISGELKGFKGIIDVVGTKPVKFQVFDMKLKKNIYEAELINGDISLQEKLFFV
                     AN"
BASE COUNT          367 a           64 c          130 g          304 t
ORIGIN      
        1 taagtcttat aaattattaa atagatttaa tttaggatta cagaatggag aacaacaaaa
       61 tatcaatgaa attaaatttt ctattggaag taaattatac ttagaaatag taaataataa
      121 aagtaaaaat ggatatattg aaggacatga tagttactct aactttatta tatatgatat
      181 agaagataac acttttaata taagtaataa atttatggat aatctaaaca aagaaaagaa
      241 tgcaaatcca aaggagattg gcttattaag taaaactaag ctagattata tggtaaaaaa
      301 caataaattg aatcttatag ttacggatat gagtaattcc ataactaatc ttgtttatga
      361 tattaattct aatgagatta aattagaaga ttatgagata cttgatttaa aagtagatta
      421 taatgaagca tcatctggag ttaggaaagt taagctactg gatgataaga tatattctat
      481 ttcaggtatt aagaacagta tatcaggaga attaaaagga tttaaaggga ttattgatgt
      541 agtcggaact aagccagtaa aatttcaagt atttgatatg aaacttaaaa agaatattta
      601 tgaagctgag ctaataaatg gagatataag tttacaagaa aagttatttt ttgttgcaaa
      661 ttaagtgtta gaattaattt tagaacttta aacttttata tgtatcacag ataagtataa
      721 gtaagggagt gtctcaaaat aaacttttta gctcatgaga cacttttttt atatgaaatc
      781 aaatatattt tcattgttta gatttatttt gataatgaaa aaaagattat ctttatttta
      841 agaaagacat ttatttataa ttata
//
LOCUS       DAYAOX010000411          849 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.417, whole genome shotgun sequence.
ACCESSION   DAYAOX010000411 DAYAOX010000000
VERSION     DAYAOX010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.417"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            606..>849
                     /locus_tag="ACGI57_003714"
     CDS             606..>849
                     /locus_tag="ACGI57_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718758.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKF"
BASE COUNT          283 a           99 c          111 g          356 t
ORIGIN      
        1 tgatttaaaa cctatttaca ctaattatct aaatatctct ttatttttat aatcttttat
       61 aacttattta tatttttcat ttttataaaa aaacaataat taagaatata actatattct
      121 acatgtaaaa atttttttat ttaaactcaa ttttctgata atttgataat ttttcaaaat
      181 ttatagttga cttttatttt ttactgtatt aaactaatta ctatacaaaa cagaaaacaa
      241 aattttcaaa attattaaat taaaaggaga ggtaaatatg aagactttat taaacaaaca
      301 agtacgattt tttagtagcc aatttagtta gggtttgtac ctaagttaag acaaattgtc
      361 ataacataga tacaagcccc ttaggattat gatttttaag gattgtatct atgttggcta
      421 tagtaattgt tgttaaagtt tttattttat catcataata tagagccaca tggattatac
      481 tgtgtggctt attcgaattt ttgccataca gttatatata ctgtgtggtt tttttatatc
      541 aaaatcattt tttaaaacca aattttaaaa accatttaaa atattttcaa aggggtgttt
      601 tatttatgtc aggtattata tctgtaatga cgcaaagttt aattttatcg attatggcac
      661 tgggggttta tataacttac aaaatcttag attttccaga tatgtctgct gatggtagct
      721 atacaatggg tgcttctatt gtagcatttt ctcttactaa tggtatttct cctgtagtag
      781 caactttaat ggctatctta tgtggttgta ctgctggtct tgttacaggg attcttcaca
      841 ttaagttta
//
LOCUS       DAYAOX010000412          832 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.418, whole genome shotgun sequence.
ACCESSION   DAYAOX010000412 DAYAOX010000000
VERSION     DAYAOX010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.418"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            119..235
                     /gene="rrf"
                     /locus_tag="ACGI57_003715"
     rRNA            119..235
                     /gene="rrf"
                     /locus_tag="ACGI57_003715"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            242..316
                     /locus_tag="ACGI57_003716"
     tRNA            242..316
                     /locus_tag="ACGI57_003716"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Asn,seq:gtt)
     gene            321..395
                     /locus_tag="ACGI57_003717"
     tRNA            321..395
                     /locus_tag="ACGI57_003717"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Glu,seq:ttc)
     gene            401..476
                     /locus_tag="ACGI57_003718"
     tRNA            401..476
                     /locus_tag="ACGI57_003718"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:434..436,aa:Val,seq:tac)
     gene            482..558
                     /locus_tag="ACGI57_003719"
     tRNA            482..558
                     /locus_tag="ACGI57_003719"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:516..518,aa:Asp,seq:gtc)
     gene            569..643
                     /locus_tag="ACGI57_003720"
     tRNA            569..643
                     /locus_tag="ACGI57_003720"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Thr,seq:tgt)
     gene            658..742
                     /locus_tag="ACGI57_003721"
     tRNA            658..742
                     /locus_tag="ACGI57_003721"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Tyr,seq:gta)
     gene            752..825
                     /locus_tag="ACGI57_003722"
     tRNA            752..825
                     /locus_tag="ACGI57_003722"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Gly,seq:tcc)
BASE COUNT          226 a          187 c          213 g          206 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      121 tggttataat agcggagaag atacacctgt ttccattccg aacacagaag ttaagttctc
      181 tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacatagc cacgtgaatg
      241 ttccaaggta gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc
      301 cccacccttg gagccattat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
      361 cggtaacagg ggttcaattc ccctacgagt caccatttgt gggatcatag ctcagctggg
      421 agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg
      481 cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtc gagggttcga
      541 gccccttcca ggtcgccaaa ataaaaatgc tggtgtggct caacggtaga gcagctgact
      601 tgtaatcagc aggttgtagg ttcgattcct atcaccagct ccatgtaaaa ctaatacgga
      661 ggatttcccg agcggccaaa gggggcagac tgtaaatctg ttgtcatcga cttcggtggt
      721 tcgaatccac catcctccac caattaaata tgcgggtgta gctcaatggt agagttctgg
      781 ccttccaagc cagctgtgag ggttcgatcc ccttcacccg ctccaaagaa ag
//
LOCUS       DAYAOX010000413          829 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.419, whole genome shotgun sequence.
ACCESSION   DAYAOX010000413 DAYAOX010000000
VERSION     DAYAOX010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.419"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            359..580
                     /locus_tag="ACGI57_003723"
     CDS             359..580
                     /locus_tag="ACGI57_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718759.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT          386 a           61 c          114 g          268 t
ORIGIN      
        1 tttaaaatca caaaaaattc ataatgaaac tgtattatat aaatataaat aagcttaaaa
       61 ccataaaata caccaactat aatgatgaga tttccttaca tcctccatat aagtcatcat
      121 tatagtaacc caacaattaa gttactatga atttaaaaaa taacaccgag catataatct
      181 atgcttagtg ttatttttat tttgaaataa aaaatattta atttattgaa atgtatagat
      241 ttagatagtt taaactatat ttaagactta aaaattataa ttgatattac aaataaatat
      301 atgtactttg ggaaaaataa atataggaag tattatattt acagaggagg tagttttgat
      361 ggatagatta aaaagagata tgaaaaatat gaaagaagat ttaaaagaag gtgttaaaaa
      421 gacagcttat gttatgaaaa atacagctga agatttgaaa gatgatgcac aaggtgctat
      481 ggtaaactta gaaatgaaaa aagatgagat gatggaaaga tatgaacaaa agaaattttc
      541 taaagaatta gagaagcaaa tgaagaatga aatgaaataa gcttttattt atgaataaca
      601 aattattgta ttcatataaa aagtagccag taatattaaa tagtttattt aaggaaatag
      661 actttctaaa aataactgta ttagatttag aaagtctatt tcaaattagt tttataaata
      721 gacataagga taaagtagat gttatagaac tagattttaa aaaatagata ctaaggataa
      781 aataaatatt atagaaatat gtttttaaga atatttatca aataaaaat
//
LOCUS       DAYAOX010000414          787 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.420, whole genome shotgun sequence.
ACCESSION   DAYAOX010000414 DAYAOX010000000
VERSION     DAYAOX010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.420"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>787)
                     /locus_tag="ACGI57_003724"
     rRNA            complement(<1..>787)
                     /locus_tag="ACGI57_003724"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          238 c          182 g          195 t
ORIGIN      
        1 cgtccatccc ggtcctctcg tactaaggac aggtcttctc aaatttccta cgcctgcgac
       61 ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaatggg
      121 cgaacagccc aacccttggg acctactaca gccccaggat gtgatgagcc gacatcgagg
      181 tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta
      241 gcttttatcc gttgagcgat ggcccttcca cgcagaacca ccggatcact aagtccgatt
      301 ttcatccttg ctcgacctgt atgtcttgca atcaagctct cttttgcctt tacactctac
      361 gtacgatttc cgaccgtact gagagaacct ttgagcgcct ccgttacctt ttgggaggcg
      421 accgccccag tcaaactgcc cacctgacag tgtcccaaga ccagattcat ggcctatggt
      481 tagagtccca gtactacaag ggtggtatcc caaggatggc tccgccaaaa ctggcgtcct
      541 ggtttcaaag cctcccacct atcctgtaca tgtagtacca agacccaatg tcaagctaca
      601 gtaaagctcc atggggtctt tccgtcctgt cgcaggtatc cggcatcttc accggaatta
      661 caatttcacc gagtctgttg ttgagacagt gcccaaatcg ttacgccttt cgtgcgggtc
      721 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
      781 actgggg
//
LOCUS       DAYAOX010000415          786 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.421, whole genome shotgun sequence.
ACCESSION   DAYAOX010000415 DAYAOX010000000
VERSION     DAYAOX010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.421"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            298..705
                     /gene="tnpA"
                     /locus_tag="ACGI57_003725"
     CDS             298..705
                     /gene="tnpA"
                     /locus_tag="ACGI57_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFG9718760.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            707..>786
                     /locus_tag="ACGI57_003726"
     CDS             707..>786
                     /locus_tag="ACGI57_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9718761.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFG"
BASE COUNT          329 a           87 c          147 g          223 t
ORIGIN      
        1 aaacttcaac gactattcct cttgagggaa gtacactaca agctatcggt agtggaagtg
       61 ggtagacctt aacggataat gccgaaggat aagatatagt ctgtgcttgc atgaaagtgt
      121 aagaagttca taagagaact gcataggtag tagcgaactt atgtgaacga caacctcaaa
      181 aatgataaaa gttaagattt gcgtttctta cgtatgttat ttaaaaataa atataaaaac
      241 cttaagaata cttgttttaa gtaattacta tgttaatatt atgttgggtg atgaaaaatg
      301 gaagtgactc atggtagagg gtatgtgtat ttagtacaat accatatagt atggtgtgta
      361 aaatatagac atccagtatt aacaaatgaa attgaagaag atttaattaa tatattaaac
      421 aaaatagctg aagataataa atttcaaata ttagaatgta atagtgataa agatcacata
      481 catttactaa taaattgttc accacaacac tatataccag atataataaa agcattaaaa
      541 ggtgtatcag caaggctgtt aatgaaaaaa catggagaac agttgaaaaa gaaattatgg
      601 ggaggacatc tttggaatcc tagttatttc gttgctactg tatcagaaaa tacagaagaa
      661 cagataagaa aatatattaa taatcagaaa aaacaagtaa agtgaggtgg cagtagtgga
      721 aaaagcttat aagtttagaa tgtatccaaa taaaaaacaa caagagttaa ttaacaaaac
      781 ttttgg
//
LOCUS       DAYAOX010000416          764 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.422, whole genome shotgun sequence.
ACCESSION   DAYAOX010000416 DAYAOX010000000
VERSION     DAYAOX010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.422"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            68..742
                     /locus_tag="ACGI57_003727"
     CDS             68..742
                     /locus_tag="ACGI57_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFG9718762.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
BASE COUNT          273 a           98 c          141 g          252 t
ORIGIN      
        1 ctatattcac ataaaaaatg tagtattata gtatatataa tattatatag aagggggtgc
       61 tagataaatg aaccatcata tattgattca atttgaatat atagcaagaa tcattactgc
      121 tggtttctgt ggggctttaa ttggatatga gagaaagaat agactaaaag aagctggaat
      181 acgcactcat tttatagtgg cattagcttc agcacttata atcattgttt ccaagtatgg
      241 atttaatgat atagtgggta ctccaggtat tggtctagac ccagcccgcg ttgcagcaca
      301 agttgtcagt ggtataggtt ttttaggtgc tggattaata tttattagaa atcaatcagt
      361 aagtggtctt actacagctg ctggtatatg ggcaacagcc ggtacaggtc ttgccattgg
      421 ctctggatta tacttagttg gtattgtttc aagtatcatt attgttattg tacagataac
      481 acttcataga gataggaaat ggatgaaact ccctacagca gagcaagttt ctcttaaaat
      541 ttctaacagc actgattcca tagaatatat tgaaggtaaa tttaaaaaat ctaatattga
      601 aattataaac atgaatatta aacgccttga tggggattgg ttagaagtag ttatatatac
      661 taagttacct aaaggatata aaaaaacaaa tttattaaaa ttatttagcg ataatcctct
      721 tatagaaact ttagaaatat aatataaaaa ttgaagctta aaaa
//
LOCUS       DAYAOX010000417          753 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.423, whole genome shotgun sequence.
ACCESSION   DAYAOX010000417 DAYAOX010000000
VERSION     DAYAOX010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.423"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            63..>753
                     /locus_tag="ACGI57_003728"
     CDS             63..>753
                     /locus_tag="ACGI57_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9718763.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTL"
BASE COUNT          278 a          105 c          127 g          243 t
ORIGIN      
        1 cattatttta tagtttgatt aatttaacaa tcactaatat atcaaactga aaggagattt
       61 ttatggaaaa aaaatatttc gaaattcatg gagttccttc aatcctttgg ggaaatcctt
      121 caagtaaatt atatatttac atacatggac aatgtggcaa taaagaggag gctgaagctt
      181 ttgctaatat tgcagttcca catgagtgtc aagttttaag tgttgatttg cctgaacatg
      241 gagaacgtaa aagagaaaaa aattcattta acccatggca tgttgttcca gaattagtat
      301 ctattatgga atatgcaaag tgccactgga atcaaatttc gttatatgca aatagcattg
      361 gagcatggtt tagtatgtta agctttgaaa aagagaactt aagagagtgt atttttgtat
      421 ctccaattct agatatgcaa cagctcattt caaatatgat gctttgggca aatgtatcag
      481 aagaacagct taaacatgaa ctaattatcc caacttcatt tgggcatact ctttcatggg
      541 aatatctaca atatgtaaaa caacatccta tcactcaatg gaatgtacca acaaaaatat
      601 tatatggtaa acatgatgaa cttactgaaa tcggtgttgt agagaaattt gtagaaaagt
      661 ttaattgcaa ccttactatt atagaaaatg gcaaacattg gtttaacaca ccacaagaat
      721 taaattcatt atatgaatgg ttaaatactt tag
//
LOCUS       DAYAOX010000418          753 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.424, whole genome shotgun sequence.
ACCESSION   DAYAOX010000418 DAYAOX010000000
VERSION     DAYAOX010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.424"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..>753
                     /locus_tag="ACGI57_003729"
     rRNA            <1..>753
                     /locus_tag="ACGI57_003729"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAYAOX010000419          749 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.425, whole genome shotgun sequence.
ACCESSION   DAYAOX010000419 DAYAOX010000000
VERSION     DAYAOX010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.425"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(48..521)
                     /locus_tag="ACGI57_003730"
     CDS             complement(48..521)
                     /locus_tag="ACGI57_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718764.1"
                     /translation="MFVICISIYLAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQ
                     ILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGG
                     IGFKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIHTYCKHQVS"
BASE COUNT          309 a          110 c           98 g          232 t
ORIGIN      
        1 aattattgta tcctctagtt tatatcacta ttatttccat caaattgtta cgatacttga
       61 tgtttgcaat aggtatgaat agccaaaaat ataaaaatag gtatttctat cactgagaaa
      121 aacagtataa tgtgtggtgt ccaaaaactc atacctccta agtgtaagaa attaaatgat
      181 gtcatttgat ataataaact aatcttaaaa ccaattcccc ctggcggtaa taggtatcca
      241 atccaactaa cacctattcc cataggaatt aagcaaatag aaaaagcaat cagcatagaa
      301 gtaagagaat ccttacattt tgcagataaa aataatgtta aactaaccat tgcaagtatt
      361 gaaattagac caccaatagc tataagaatc tgtgcttgta gtaaatttat atttactaga
      421 ctaatgacag aaaacaacat ttgaaatgag gttttcaaac attcagtacc aaaagctaaa
      481 tcagaaattg ccaaatagat agaaatacat ataacaaaca ttattataaa tatagaaaat
      541 agagctaata ttttagtaaa cgctagacaa atatgtccat gtttcgtaca acgaaggatg
      601 ctatctgatt gtgtctgata ctcaccagaa aaagtaggtg cagcaattgc aacatatata
      661 attaataata taaaaatata aaatattata taatcgaaag catcttctgg atttattaag
      721 aaattcttaa tcttattatt taactattt
//
LOCUS       DAYAOX010000420          747 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.426, whole genome shotgun sequence.
ACCESSION   DAYAOX010000420 DAYAOX010000000
VERSION     DAYAOX010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.426"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            15..105
                     /locus_tag="ACGI57_003731"
     tRNA            15..105
                     /locus_tag="ACGI57_003731"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49..51,aa:Ser,seq:gct)
     gene            114..190
                     /locus_tag="ACGI57_003732"
     tRNA            114..190
                     /locus_tag="ACGI57_003732"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:148..150,aa:Pro,seq:tgg)
     gene            276..351
                     /locus_tag="ACGI57_003733"
     tRNA            276..351
                     /locus_tag="ACGI57_003733"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:309..311,aa:Trp,seq:cca)
     gene            412..488
                     /locus_tag="ACGI57_003734"
     tRNA            412..488
                     /locus_tag="ACGI57_003734"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:446..448,aa:Pro,seq:tgg)
     gene            495..571
                     /locus_tag="ACGI57_003735"
     tRNA            495..571
                     /locus_tag="ACGI57_003735"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:529..531,aa:Ile,seq:gat)
     gene            575..650
                     /locus_tag="ACGI57_003736"
     tRNA            575..650
                     /locus_tag="ACGI57_003736"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:608..610,aa:Phe,seq:gaa)
     gene            658..734
                     /locus_tag="ACGI57_003737"
     tRNA            658..734
                     /locus_tag="ACGI57_003737"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Met,seq:cat)
BASE COUNT          176 a          171 c          215 g          185 t
ORIGIN      
        1 aaatgactga atttggagaa gtactcaagt ggctcaagag gatcccctgc taagggatta
       61 ggctgggtaa ccggtgcgag ggttcgaatc cctccttctc cgccattcaa aaacgaggtg
      121 tagcgcagtt tggtagcgca catggtttgg gaccatgggg ccgggggttc gagtcccttc
      181 acctcgacca acttaagctt ttagttcagt tggttagagt gcccaactca tggctagtaa
      241 ttctagggtt tgagtccatg aaggttattc ttcatagggg tgtagctcag ttggtagagc
      301 gtcggtctcc aaaaccgtgc gccgggggtt cgagtccctc cacccctgcc aaataaaaag
      361 gacttgaaaa aagttggatt ttagagtagt ataagatagt aaataagatt tcgaggtgta
      421 gcgcagtttg gtagcgcaca tggtttggga ccatggggcc gggggttcga gtcccttcac
      481 ctcgaccaag aaaagggcct atagctcagg tggttagagc gcacgcctga taagcgtgag
      541 gtcgctggtt cgagtccagc taggcccacc atttgcccag atagctcagt cggtagagca
      601 ggggactgaa aatccccgtg tcggtggttc gattccgcct ctgggcacca ggaatacggc
      661 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      721 tattaccgct accatctaac tatatca
//
LOCUS       DAYAOX010000421          743 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.427, whole genome shotgun sequence.
ACCESSION   DAYAOX010000421 DAYAOX010000000
VERSION     DAYAOX010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.427"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            187..>743
                     /locus_tag="ACGI57_003738"
     CDS             187..>743
                     /locus_tag="ACGI57_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9718765.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDV"
BASE COUNT          284 a           94 c          123 g          242 t
ORIGIN      
        1 acttagttta taaataatca atataattta tccaatatta cacattactg aaaaaactaa
       61 acataaagct gaaaattatt attaagtttt tgtagaaata ttataagcat agatacattt
      121 ttgtaacaac tacaataaat ttttaatact atatactatt cgtatgtctt atgggaggta
      181 tttattatgt ttaaaaaaat agtcgcaata gcaagctcta tcattgtaag tacaatatgt
      241 ctaactggat gtgattttaa ctctaatgac aaaaaaggtg aatcttcttc gtctaaaaat
      301 ctggataaag taactatagc agaagttact cactctgttt tttatgctcc acagtatgct
      361 gctattacga aaggattctt tgaagatgaa ggtataaaga ttgaccttat aaatacccaa
      421 ggtgcagata aaactatggc agctttgatt tcaggtgaag caaacgtagg tttaatgggt
      481 cctgaagcat ctatatatgt atataatcaa aacaaaaacg attatgcagt gaactttgct
      541 caattgacaa aaagagatgg tagttttcta gttgctagga aaaaaatgcc taatttctct
      601 tacaatgatt taaaaggaac cgaaatatta ggtggtagaa aaggtggagt tccactaatg
      661 acttttgaat atgttctaaa agaaaaaggt cttactattg gagaaaatct aaaagcaggc
      721 aatgttaatg taagaactga tgt
//
LOCUS       DAYAOX010000422          694 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.428, whole genome shotgun sequence.
ACCESSION   DAYAOX010000422 DAYAOX010000000
VERSION     DAYAOX010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.428"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
BASE COUNT          301 a           63 c           67 g          263 t
ORIGIN      
        1 aaatttttaa ttagacttct ctcttattca agatttttct atataaaatc taagaataaa
       61 tagaaaacta acatctaacc ttttattttt tattttcgat ctatttactt gaattttaac
      121 ataaatctat cttttagtat atattaatca tgctgaattt gtgatgacat gtatacaaaa
      181 tatcgtttag tattttctaa taaaaaatat tactgaaatt aaaaatctac aatagcattt
      241 aacaagtaaa aacatgttta cacaaatata taatttaagg tctttaaaat agagtatgta
      301 ttattctaaa aattagttat agttatcaca taaaaataga aaagaatttt caaaagattt
      361 aaagaacata tatacttcaa ttgcagaaga tgtaatgtta agtgaactag cattcttcga
      421 ggaaaaatag actcaaaaaa ttagaaaata gaatcaggtt ttagatgatt taagtattta
      481 ttttcaaaaa agaaatttga ttctaagtta ataaagaaat atttaaaaaa atcaaaaaat
      541 tttcttaaat aagtaatagg ttttgtaaaa atggaaaaat atcaaaataa gactatgatt
      601 tttaaaacta tagccttatc aataaatttt tatttaaaga acacacataa atatttttaa
      661 acctaattta tgtgaagttt tatgtatttt ttat
//
LOCUS       DAYAOX010000423          672 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.429, whole genome shotgun sequence.
ACCESSION   DAYAOX010000423 DAYAOX010000000
VERSION     DAYAOX010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.429"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(135..335)
                     /locus_tag="ACGI57_003739"
     CDS             complement(135..335)
                     /locus_tag="ACGI57_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFG9718766.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT          294 a           77 c           61 g          240 t
ORIGIN      
        1 taaaaaagtt agtttaagtt tgacgctttt ctaaagtaac ttcttatttt tgtaattaaa
       61 aataaatata ataaacaaca aaaaagagcc ctcgatagct ccttttctaa aatatatata
      121 tttaaattaa ataattatat tctatttacg ttttcagctt gaggtcctct attaccttga
      181 gttatatcaa agctaacctt ttcgccttct tctaaagttt tgtatccatc attttgtata
      241 gctgagaaat gtacgaaaac atcatcttct ccctctacag atatgaaccc aaatcctttt
      301 tcattgttaa accattttac tattccgttt ttcattataa gaaaacctcc gcattattaa
      361 aaaatacaaa ctaaaaaaat aagtctgttc tattaatata acataaattt taaataaaag
      421 caaataaaat ttttaaaaaa attaattact tttagaattt aataaaaatt gttaaattat
      481 acagtgagaa gtattgtaaa atttgacgaa aatgtaaatg tagataataa atttaatatc
      541 aaaaaatgta acaaaaaggt atgtaagtaa aaagtaaaat taaaatatat aataaaaaat
      601 attatatatt agattgaaaa gtaaaaataa aaatagtgaa atttcagaaa tatcaataat
      661 attatataat aa
//
LOCUS       DAYAOX010000424          670 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.430, whole genome shotgun sequence.
ACCESSION   DAYAOX010000424 DAYAOX010000000
VERSION     DAYAOX010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.430"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            60..614
                     /gene="tcdR"
                     /locus_tag="ACGI57_003740"
     CDS             60..614
                     /gene="tcdR"
                     /locus_tag="ACGI57_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HFG9718767.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
BASE COUNT          289 a           46 c           89 g          246 t
ORIGIN      
        1 ccaatgttgt caaaattttc aaataaatca tcattataaa tataagagag gatgatttta
       61 tgcaaaagtc tttttatgaa ttaattgttt tagcaagaaa taactcagta gatgatttgc
      121 aagaaatttt atttatgttt aagccattag taaaaaaact tagtagagtt ttacattatg
      181 aagagggaga aacagattta ataatatttt ttattgaatt aataaaaaat attaaattaa
      241 gtagcttttc agaaaaaagc gatgctatta tagtcaaata tattcataaa tcattactga
      301 ataagacttt tgagttgtct agaagatatt ctaaaatgaa gtttaatttt gtagaatttg
      361 atgaaaatat cttaaatatg aaaaataatt atcaaagtaa gtctattttt gaggaagata
      421 tttgtttttt cggatatatt ttgaaagaat tatctggtat tcaaagaaaa gttatttttt
      481 ataaatattt aaaaggatat tctgatagag aaatatcagt gaaattaaaa atatctagac
      541 aagctgttaa taaggctaaa aatagagcat ttaaaaaaat aaaaaaagac tatgaaaatt
      601 attttaactt gtaattaatg agtttaaaga aatatttaca atagaaatca aattttataa
      661 ttaactttat
//
LOCUS       DAYAOX010000425          665 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.431, whole genome shotgun sequence.
ACCESSION   DAYAOX010000425 DAYAOX010000000
VERSION     DAYAOX010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.431"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..>665
                     /locus_tag="ACGI57_003741"
     CDS             <1..>665
                     /locus_tag="ACGI57_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9718768.1"
                     /translation="KSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGT
                     INYNQDPEYLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSR
                     KDVNKYLRKTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPL
                     ATTYNAPILLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQT
                     RLNG"
BASE COUNT          295 a           93 c          106 g          171 t
ORIGIN      
        1 aaatcaaaag ttagtgtcaa atttaaagaa aaaccagatg cagatagcat tacattaaag
       61 tataaatgct atgacatgcc attgaataca actctaaatt acaatcaatc aactggggca
      121 tatgaaggaa ctatcaatta taaccaagac ccagaatatc taaatgtttg ggaactacaa
      181 gggataacaa taaacagcaa aaataatcct aaaactttaa acagacaaga cctagaaaag
      241 ctgggattaa atttaaaaga ctataatgta acacaggaat gtataattga agatataact
      301 tctagaaaag atgtaaataa atatttgaga aaaacttctt cacctattac agaacttaca
      361 ggaagtgata gatatgaaac agcagttaaa ataagtaaag agggctggaa aaatggttca
      421 gataaggtag ttataataaa tggggatgta agtatagatg gcattatatc aactccactg
      481 gcaaccacat ataatgcacc aatacttttg gttgaaaaaa acaatgtacc taatagtgta
      541 aaatcagaat taaagcgcct aaaccctaaa gatataatta taattggaga tgagaatgct
      601 atttctaaaa ctactgctaa tcaaattaaa tcaactgtaa atgctagtca aacacgttta
      661 aatgg
//
LOCUS       DAYAOX010000426          665 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.432, whole genome shotgun sequence.
ACCESSION   DAYAOX010000426 DAYAOX010000000
VERSION     DAYAOX010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.432"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..>665
                     /locus_tag="ACGI57_003742"
     CDS             <1..>665
                     /locus_tag="ACGI57_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9718769.1"
                     /translation="TSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGT
                     INYNKDPEYLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSR
                     KDVNKYLRKTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPL
                     ATTYNAPILLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQT
                     RLKG"
BASE COUNT          294 a           87 c          109 g          175 t
ORIGIN      
        1 acctcaaaag tcagtgttaa gtttaaagaa aaaccagatg cagatagcat tacattaaag
       61 tataaatgct atgatatgcc attggataca actttaaact ataatcaatc aactgagtca
      121 tatgaaggaa ctatcaatta taataaagac ccagaatatc taaatgtttg ggaactacaa
      181 gggataacaa taaacagcaa aaataatcct aaaactttaa acaaacaaga actagaaaag
      241 atgggattaa atttaaaaga ctataatgta acgcaggaat gtataattga agatataact
      301 tctagaaaag atgtaaataa atatttaaga aaaacttctg cacctattac agaacttaca
      361 ggaagtgata gatacgaaac agcagttaaa ataagtaaag aaggctggaa aaatggttca
      421 gataaggtag ttataataaa tggggatgta agtatagatg gcattatatc aactccacta
      481 gcaactacat ataatgcgcc aatacttttg gttgaaaaaa acaatgtacc taatagtgta
      541 aaatcagaat taaagcgttt aaatcctaga gatgtaatta taattggaga tgagaatgct
      601 atttctaaaa ctactgctaa tcaaattaaa tcgactgtaa atgctagtca aacacgtcta
      661 aaggg
//
LOCUS       DAYAOX010000427          664 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.433, whole genome shotgun sequence.
ACCESSION   DAYAOX010000427 DAYAOX010000000
VERSION     DAYAOX010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.433"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>664)
                     /locus_tag="ACGI57_003743"
     rRNA            complement(<1..>664)
                     /locus_tag="ACGI57_003743"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAYAOX010000428          664 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.434, whole genome shotgun sequence.
ACCESSION   DAYAOX010000428 DAYAOX010000000
VERSION     DAYAOX010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.434"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            106..204
                     /locus_tag="ACGI57_003744"
     CDS             106..204
                     /locus_tag="ACGI57_003744"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718770.1"
                     /translation="MFILKRLCKLVSINKSFSEEIPKNLKNTYMCI"
BASE COUNT          266 a           53 c           76 g          269 t
ORIGIN      
        1 tggtgtattt taaaattggt ataagcatta caaatacgaa tatgccaact gtaatatgta
       61 atataagtag ctatatacat atgtgctgtc ccatatattg tatcattgtt tatattaaaa
      121 agattatgta aattggtatc aatcaataaa tcattttcag aagaaatacc taaaaactta
      181 aaaaatacct atatgtgtat ttagttagat ttgagatttt tacatttaat tttatagtga
      241 caatattata ttatttttat gatgtctatt tatatgcaat tactataata tcagaactta
      301 tagtatcatt tgtttcatta tcaataaggt tttttacttt agttttgtca attttattaa
      361 aaaaagttat tgatataaaa gataagaagg ataaaactat atgacggatt tcaaacataa
      421 gagtggatat ttatataata gatattttat agttaatttt attagatttt atttaatact
      481 gtaatattaa tatcacaata tattaatagt gtattaaaga ttgaattttg tagataacta
      541 taatttattg agagtgataa ataaaaatta tataatatta cagaagaaag agagggagac
      601 aatattatat ccctcttttt tctataataa ctaattatta aaaaattatt aatactctat
      661 atac
//
LOCUS       DAYAOX010000429          646 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.435, whole genome shotgun sequence.
ACCESSION   DAYAOX010000429 DAYAOX010000000
VERSION     DAYAOX010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.435"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            139..429
                     /gene="cas2"
                     /locus_tag="ACGI57_003745"
     CDS             139..429
                     /gene="cas2"
                     /locus_tag="ACGI57_003745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFG9718771.1"
                     /translation="MKKKNNLNYNYVFVVYDVGEKRVSKVFKVCKKYLSHFQKSVFRG
                     EITPSKIMSLKRDLENIIDKDNDFVCIIKLMNSNTFEEEILGNNSNCENMIL"
BASE COUNT          273 a           39 c           94 g          240 t
ORIGIN      
        1 atacataata tattattgaa ataatataag tttttagaag tattttaaga tgagaaagga
       61 gaaaatactg aaaaataatt attctattat aatttgccaa ggttttgtat atttttcagt
      121 ttaaatatta tattacatat gaaaaagaaa aacaatttaa attataatta tgtatttgta
      181 gtctatgatg taggagaaaa aagagtaagt aaagttttta aagtgtgtaa aaaatatctc
      241 tcgcattttc aaaaatcagt ttttagaggt gaaataactc catctaagat tatgagctta
      301 aaacgtgatt tagaaaatat tatagataaa gataatgatt ttgtatgtat aataaagttg
      361 atgaattcta atacatttga agaagaaata ttagggaata atagtaattg tgaaaatatg
      421 atattataaa tctaattact atatttgtaa aaaataatag cttattaaag agtagtaaaa
      481 ttgggatata atttatgata gagactgcaa gtagattttt gtgaaaagga tttacttgca
      541 gtatttttgt tatgtataac tgttaacatt agtgtacaaa acattatcaa tagcattttt
      601 ctataatgaa gaaatactat tataatggga aaattttatt aatact
//
LOCUS       DAYAOX010000430          628 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.436, whole genome shotgun sequence.
ACCESSION   DAYAOX010000430 DAYAOX010000000
VERSION     DAYAOX010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.436"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            171..320
                     /locus_tag="ACGI57_003746"
     CDS             171..320
                     /locus_tag="ACGI57_003746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718772.1"
                     /translation="MEIVAVYYIAKFLLDLYKVKSHSKSEAKSGFIFNLTINKLIVKI
                     KYKKY"
BASE COUNT          243 a           71 c           81 g          233 t
ORIGIN      
        1 tgttttttat tgcaatatag aatatattgt ataaataaaa aaatatggag aatggatatt
       61 tgataattgt aaaattatgt tataatttat atgtaaggaa ctaaatatct aagagtggca
      121 gtttccacat tatgctctaa cctactttct aatgaaagga ggtgggaagt gtggaaatag
      181 tagcagttta ttacatagca aaatttttac ttgatttata caaagtaaaa agccactcaa
      241 agtcggaagc taagagtggc tttatattta atttaactat taataaacta atagtaaaaa
      301 ttaaatataa aaagtactaa attttttcgg aaactacact ctcgacaata gattatagtt
      361 ccttatttct aaagaggtag gagaattgca gttctcttat ctctttttga tacgaattta
      421 catctttatt atatcgcatt ttgagaaaaa ataaaactat gaatattaga aatatatttt
      481 attttatctc taagtatata tattattagc cacctaaaaa actttcacaa aaacaaaaag
      541 agtatatgtt tttgataact ggatgatata acttataact cattttacaa ttcttagttc
      601 atatatattt atgccactaa atttctct
//
LOCUS       DAYAOX010000431          627 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.437, whole genome shotgun sequence.
ACCESSION   DAYAOX010000431 DAYAOX010000000
VERSION     DAYAOX010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.437"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
BASE COUNT          271 a           42 c           86 g          228 t
ORIGIN      
        1 catataatat ttcaagcttg tataatgtaa caattaaacc aaaagactca ttgtatattt
       61 gaaattaagt tttttatagt ttataggtgt tttatgtgga ttacgaatga tatagtattg
      121 atacattatt taatagattc ttatgccaag aaacaattaa tttatagaaa ttcttaattg
      181 ttacaatata aatatctaga taagttataa gatactgata aataatttat aaagtttatt
      241 aatctaatat tgtgtgtgaa tattaagtgg tcaagtagtc taaaaaatga aaaagattta
      301 aaaaagtgtt ttaactaaac acataagtgt taaattcatg tataagtata ggttagatac
      361 ataagaaata attatgtaat atctatcttt gtaaaaaaat attaaatttt gtaatttgtg
      421 gataaatatg tttataatgt ggataatatt taatgtaata agttaaagca atatgaatca
      481 actgtttata atgtggataa tattataaat gtatgaataa ttgcagtatt taaaaacaaa
      541 aaaagttaaa aaagttgttg actctaaaag aaaaagttgg tatagtaata cttgtcctca
      601 agaaaagggc aaacaaataa agcataa
//
LOCUS       DAYAOX010000432          619 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.438, whole genome shotgun sequence.
ACCESSION   DAYAOX010000432 DAYAOX010000000
VERSION     DAYAOX010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.438"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            29..>619
                     /locus_tag="ACGI57_003747"
     CDS             29..>619
                     /locus_tag="ACGI57_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718773.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLG"
BASE COUNT          254 a           58 c          103 g          204 t
ORIGIN      
        1 gcaaataata cttcaaggag aatgtactat gacgaatatg gaaaaagaga tattactttt
       61 aattagcagg gttagatgtg ttacagagtc tcaatttaga aaaatttatg gagaccaaaa
      121 aagatacaat aaaaaaaatt ttaaaaaaac tttgagaaaa atgtgtagtg aatatacact
      181 aaaaaaatat ccttgtgatg tggattatta taattataaa gacctctctt atgtatacta
      241 tttaaacggt agtaaacttt ttaagggaga tgatttaata aaggctctag taggatcaga
      301 attagttgtt agaatcaata ctgcaggttg tgatgtaaaa agattttacc gaaatgttag
      361 tgtagatgat gtgaaatacg atttatttat tgaatataca gaccaatttg gtaatataaa
      421 gcaagtttta gttgatgttg acttaaataa tgaaatagac atttctaaat acgaaaatct
      481 ttctaaaaaa atagataaat ctacaattcc attttttaaa gtaccaaacg ttttggtagt
      541 tacaccagat aatctatccg aattaaatga tttagagaga tatgataatg agtatagcat
      601 aaattttata gatttagga
//
LOCUS       DAYAOX010000433          577 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.439, whole genome shotgun sequence.
ACCESSION   DAYAOX010000433 DAYAOX010000000
VERSION     DAYAOX010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.439"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..>577
                     /locus_tag="ACGI57_003748"
     rRNA            <1..>577
                     /locus_tag="ACGI57_003748"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAYAOX010000434          566 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.440, whole genome shotgun sequence.
ACCESSION   DAYAOX010000434 DAYAOX010000000
VERSION     DAYAOX010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.440"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(298..414)
                     /gene="rrf"
                     /locus_tag="ACGI57_003749"
     rRNA            complement(298..414)
                     /gene="rrf"
                     /locus_tag="ACGI57_003749"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(423..496)
                     /locus_tag="ACGI57_003750"
     tRNA            complement(423..496)
                     /locus_tag="ACGI57_003750"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(462..464),aa:Gly,seq:tcc)
BASE COUNT          216 a           95 c           65 g          190 t
ORIGIN      
        1 taataatttt gtatttcttc tcatattaat ctccttattt aacataaatt tctactttgt
       61 tttttaacct tttaattttt gaaattatat caaagtgttt ttacccaaaa ttataaataa
      121 gtacataaaa aataaacttc taaaaaaatt ataaaaacaa aaaataaaaa aattaaatat
      181 attgaatata tttaattatc ttcactatat catttattgc caaaaaaatc tataaaagac
      241 aaataaaaac atataacaat tgtaggttta ttaaatttaa tacaacaaaa aaagattacg
      301 tggctacgtc ctactctccc aggcagtttc ccaccaagta ccatcagcgc tagagagctt
      361 aacttctgtg ttcggaatgg gaacaggtgt atcctctctg ctattgtaac cacataatct
      421 tttggagcgg gtgaagggga tcgaaccctc acagctggct tggaaggcca gaactctacc
      481 attgagctac acccgcatta aatttttata aattagtaaa actaattttt tagagttagc
      541 actctaaaaa ctacatatat atccta
//
LOCUS       DAYAOX010000435          561 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.441, whole genome shotgun sequence.
ACCESSION   DAYAOX010000435 DAYAOX010000000
VERSION     DAYAOX010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.441"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
BASE COUNT          187 a           70 c           56 g          248 t
ORIGIN      
        1 agtaaaacta aaggatgatg ctacaacatt ccagttattt cccaaaattt taatttctga
       61 agtatactgt ttacattatt tttaatctat tttatctaaa gtcgataagg cttcttattc
      121 attaactgac ctatatacag atttaaaatt acttattaat tcttttgagt ccttgtatga
      181 aacatattta aagtatttct tatctaatgt attaaacatt tttgtattac agaatgagga
      241 taacttgtag ctattgtctc tttgaaataa gtcatctgtg tttactccta aaactactta
      301 tatctgtaga ttttataagt tctccatctt atataatttc tttgattaat tgttttctta
      361 aattattcat aaaaaaatct ccgttacaat atttgattat atttaaatca ttgctataaa
      421 gaagactttt attaactttg aaaaacaaat tattttaatt gctggtgatt agttgctacc
      481 ttcttttgat attaattcta ttagatttta tatagttata tatctctttc taactaagta
      541 atataatatt actgttatag t
//
LOCUS       DAYAOX010000436          545 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.442, whole genome shotgun sequence.
ACCESSION   DAYAOX010000436 DAYAOX010000000
VERSION     DAYAOX010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.442"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            284..448
                     /locus_tag="ACGI57_003751"
     CDS             284..448
                     /locus_tag="ACGI57_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718774.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT          213 a           39 c           85 g          208 t
ORIGIN      
        1 atttttatga actagaatat tctttataaa ttcatagcag ttttaataga taactttttc
       61 agatagatta aacaaagttt tatttactgt attctattat cagtttccta caaattaaat
      121 tgtctatttt tatttataga gtagaataaa ctgtttttgt taagaatttt gaaggtaaag
      181 ctagtttaaa aaaataaagg gactaatttg tctaacgttt attggtgaaa aataagtcta
      241 tagaagttat aataaatata taaaaatgta tgagaggtgt tatatgtttg gtaaaccaaa
      301 agaaagtgtt agatttttag actttaatga tgtagcagaa aagtttggag agaaaaaggc
      361 tgttgcaatt tttgatgcca tagctggaaa aaaatctaaa gaacatatta tcaaagatat
      421 agaaaaatta gtaaaaaatg aagtttagat atttataagg tatattgagc ttgagggtag
      481 ctctttatac cttctttttt atgattttta gataatattt taaaatttaa ataagaaact
      541 tattg
//
LOCUS       DAYAOX010000437          524 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.443, whole genome shotgun sequence.
ACCESSION   DAYAOX010000437 DAYAOX010000000
VERSION     DAYAOX010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.443"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(303..503)
                     /locus_tag="ACGI57_003752"
     CDS             complement(303..503)
                     /locus_tag="ACGI57_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFG9718775.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
BASE COUNT          190 a           67 c           54 g          213 t
ORIGIN      
        1 aattactaaa aatgttatat cagaattgaa tacaaaagaa agagtatgta tttatgagat
       61 agttgaaaat tttgatgtgc cacaactaaa attattatat tactatatat ttaattaaaa
      121 gagtacttgt aggtttaagt acatacttta taaatatatt tttgttagat ttattttata
      181 aaaagtattt agactaagta ttatttttaa gtattttctt gttgaagtta ttttttcctt
      241 ttttacaaaa ttcgtaaata aaaaaaagac tcttaaaaga gtctttccga aaaatctata
      301 tattataata tagttatatt ttctgcttga ggacctctag cgcctttaac tatatcaaag
      361 cttacttttt gaccttcttc taatgattta aatcctgaag tttgtatagc tgagaaatga
      421 gcaaatacgt catctccact ttccattgat ataaatccaa atcctttttc attattaaac
      481 cattttacta ttccattatt cattaaaaat actcctatta acct
//
LOCUS       DAYAOX010000438          519 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.444, whole genome shotgun sequence.
ACCESSION   DAYAOX010000438 DAYAOX010000000
VERSION     DAYAOX010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.444"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            2..75
                     /locus_tag="ACGI57_003753"
     tRNA            2..75
                     /locus_tag="ACGI57_003753"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Met,seq:cat)
     gene            90..166
                     /locus_tag="ACGI57_003754"
     tRNA            90..166
                     /locus_tag="ACGI57_003754"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Ile,seq:cat)
     gene            190..266
                     /locus_tag="ACGI57_003755"
     tRNA            190..266
                     /locus_tag="ACGI57_003755"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:224..226,aa:Pro,seq:tgg)
     gene            274..350
                     /locus_tag="ACGI57_003756"
     tRNA            274..350
                     /locus_tag="ACGI57_003756"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:308..310,aa:His,seq:gtg)
     gene            359..434
                     /locus_tag="ACGI57_003757"
     tRNA            359..434
                     /locus_tag="ACGI57_003757"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:392..394,aa:Lys,seq:ttt)
     gene            442..515
                     /locus_tag="ACGI57_003758"
     tRNA            442..515
                     /locus_tag="ACGI57_003758"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:474..476,aa:Cys,seq:gca)
BASE COUNT          119 a          120 c          142 g          138 t
ORIGIN      
        1 tggcggtata gcttagttgg ctagagcgtt cggttcatac ccgaaaggtc ataggttcga
       61 ctcctattac cgctactaaa atttaatgtg ggccattagc tcagttggtt agagcgcccg
      121 gctcataacc ggtaggtctg gggttcgagt ccctgatggc ccaccacatt aaattaatta
      181 atataaattc gaggtgtagc gcagtttggt agcgcacatg gtttgggacc atggggccgg
      241 gggttcgagt cccttcacct cgaccaatat aaggtgggta tagctcagtt ggttagagcg
      301 ccagattgtg gctctggagg tcgtgagttc gactctcatt atccacccca attcatatgg
      361 tctattagct cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg
      421 cggatagatc accaaaaagg tggcgacata gccaagtggt aaggcagtgg actgcaactc
      481 cttgatcccc agttcgaatc tgggtgtcgc ctccaaaat
//
LOCUS       DAYAOX010000439          496 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.445, whole genome shotgun sequence.
ACCESSION   DAYAOX010000439 DAYAOX010000000
VERSION     DAYAOX010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.445"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..>496
                     /locus_tag="ACGI57_003759"
     CDS             <1..>496
                     /locus_tag="ACGI57_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFG9718776.1"
                     /translation="GTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLD
                     QAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFD
                     GYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRT
                     VASGV"
BASE COUNT          213 a           61 c          125 g           97 t
ORIGIN      
        1 ggaacagttg caacaggaag agtggaaaga ggagtactaa aagtacaaga cgaagtagaa
       61 ttagtaggat taacagaagc accaagaaaa gtagtagtaa caggagtaga gatgttcaga
      121 aaattattag accaagcaca agcaggggat aatataggag cattattaag aggagtacaa
      181 agaaacgaga tagaaagagg acaagtacta gcaaagactg gatcagtaaa ggcacacaca
      241 aagtttacag cagaagtata tgtacttaaa aaagaagaag gtggaagaca tacaccattc
      301 tttgatggat atagaccaca attctatttc agaacaacag acgtaacagg agcttgtaag
      361 ttaccagaag gaatagagat ggtaatgcct ggagataacg taacaatgga agtagactta
      421 ataaactcaa tagttgtaga agagggatta agattctcaa taagagaagg tggaagaaca
      481 gtagcttcag gagttg
//
LOCUS       DAYAOX010000440          493 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.446, whole genome shotgun sequence.
ACCESSION   DAYAOX010000440 DAYAOX010000000
VERSION     DAYAOX010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.446"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="ACGI57_003760"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="ACGI57_003760"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="ACGI57_003761"
     tRNA            310..398
                     /locus_tag="ACGI57_003761"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
     gene            410..486
                     /locus_tag="ACGI57_003762"
     tRNA            410..486
                     /locus_tag="ACGI57_003762"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ile,seq:cat)
BASE COUNT          156 a           91 c          109 g          137 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgaa tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggtt agagcgcccg gctcataacc ggtaggtctg gggttcgagt ccctgatggc
      481 ccaccacaat aat
//
LOCUS       DAYAOX010000441          480 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.447, whole genome shotgun sequence.
ACCESSION   DAYAOX010000441 DAYAOX010000000
VERSION     DAYAOX010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.447"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            3..79
                     /locus_tag="ACGI57_003763"
     tRNA            3..79
                     /locus_tag="ACGI57_003763"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Asp,seq:gtc)
     gene            90..164
                     /locus_tag="ACGI57_003764"
     tRNA            90..164
                     /locus_tag="ACGI57_003764"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:122..124,aa:Gly,seq:gcc)
     gene            174..247
                     /locus_tag="ACGI57_003765"
     tRNA            174..247
                     /locus_tag="ACGI57_003765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Arg,seq:tct)
BASE COUNT          149 a           83 c          102 g          146 t
ORIGIN      
        1 gcggcctggt agttcagttg gttagaatgc cagcctgtca cgctggaggt cgagggttcg
       61 agccccttcc aggtcgccaa attaaataag cgggaatagt tcagtggtag agcgcaacct
      121 tgccaaggtt gaagtcgcga gttcgaatct cgtttcccgc tccataataa cttgggtgca
      181 tagctcagct ggatagagca acgcccttct aaggcgtgtg tccggggttc gaatccctgt
      241 gcgctcatta agaaaagttt actataaatt atagtaaact tttatattgt ttaaaaagac
      301 acaggaaaca tgtatattac ctgtgtcttt ttatttataa actttaaact atgtaaaaat
      361 tacagtatat tctaatagag ttacagtata ttcattaata ttgtaaacat acggagggta
      421 atgtataata gtctacaaga tgtttatgaa ggcatcagtt aaatataata aatataacta
//
LOCUS       DAYAOX010000442          474 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.448, whole genome shotgun sequence.
ACCESSION   DAYAOX010000442 DAYAOX010000000
VERSION     DAYAOX010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.448"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..>474
                     /locus_tag="ACGI57_003766"
     CDS             <1..>474
                     /locus_tag="ACGI57_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFG9718777.1"
                     /translation="DKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYK
                     KFFKEKAGFPKFKSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPKGRI
                     LNATISKEPSGRYYVSLCCIDVDIEVFENTNNHIGLDLGIKEFCISSCGEFIENP"
BASE COUNT          202 a           50 c           70 g          152 t
ORIGIN      
        1 acgataaaga aacttttact tataaacagt gtagttctga tttgaccaat ttaaaaaaag
       61 aattaaaatg gcttaaagaa cctgacaaat tttcactaca aaacgcctta aaagatttag
      121 ataatgcata taaaaaattc tttaaagaaa aagcaggatt tcctaaattt aaatcaaaga
      181 aaattaatag attttcatat aaaactaatt ttacaaatgg aaacattatg tattgtggtc
      241 aacatataaa actacctaaa cttggtatgg ttaagataag ggataaacaa gtacctaaag
      301 ggagaatact taatgctact atatctaaag aaccaagtgg tagatattat gtatcattat
      361 gctgtattga tgtagatatt gaagtatttg aaaatactaa taatcatata ggtttagatt
      421 tgggaattaa agaattttgt atctcaagtt gtggagaatt tatagaaaat ccta
//
LOCUS       DAYAOX010000443          465 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.449, whole genome shotgun sequence.
ACCESSION   DAYAOX010000443 DAYAOX010000000
VERSION     DAYAOX010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.449"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..342
                     /locus_tag="ACGI57_003767"
     CDS             <1..342
                     /locus_tag="ACGI57_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718778.1"
                     /translation="SLMAQFLGFSDVNMGIGTLVLGIASIIIGITLFKKFTFIKDTTA
                     IIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIISFLATGNLNISLKKNKCKASTKNK
                     SEKRGVVDVTN"
BASE COUNT          170 a           65 c           70 g          160 t
ORIGIN      
        1 agtcttatgg ctcagttctt gggattctca gatgtaaata tgggtatagg aactttagtt
       61 cttggaattg cctctatcat aattggtatt acactattta agaaatttac ttttatcaaa
      121 gatactactg caataattgt tggctctttt atataccaat tcactatata ctttgcaatg
      181 agtttaggaa tgttatcaac tgacttaaaa ctaataactg ccatagttat aatatctttc
      241 ctagctactg gtaacttaaa tatttcacta aaaaaaaaca aatgcaaagc tagtaccaaa
      301 aataaatcag aaaaaagagg tgttgtagat gttacaaatt aaaaatcttt caaagagttt
      361 ccctaatcca tatggtgaac ctaatactat ctttgaaaat ttatcaatcg atatagaaga
      421 tggtgaattt gtaagtatca taggtagtaa tggaactggt aaatc
//
LOCUS       DAYAOX010000444          433 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.450, whole genome shotgun sequence.
ACCESSION   DAYAOX010000444 DAYAOX010000000
VERSION     DAYAOX010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.450"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..>433
                     /locus_tag="ACGI57_003768"
     CDS             <1..>433
                     /locus_tag="ACGI57_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFG9718779.1"
                     /translation="CISSCGDFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARL
                     KVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSE
                     FVRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNEEMKDL"
BASE COUNT          184 a           49 c           74 g          126 t
ORIGIN      
        1 tgtatctcaa gttgtggaga ctttatagaa aatcctaagt atcttaagaa gtcattaaac
       61 aaacttgcta aattacaaag agaattatcg agaaagacaa tcggtagttt aaatagaaat
      121 aaagcaagat taaaagttgc aagacttcaa gaacatatag ctaatcaaag aaatgatttt
      181 ctacaaaaac tatctactaa attaataaaa gagaatgata ttatttgcat agaagattta
      241 caagtaaaga atatgattag aaatcgtaaa ctatctcgct taatttctga tgtgtcatgg
      301 tcagaatttg ttcgtcaatt aaaatacaag gctaattggc atggtagaca aattgtaaag
      361 gtaggtaaat tctttgcaag ttcacaaata tgcaataaat gtgggtataa aaacgaggaa
      421 atgaaggact taa
//
LOCUS       DAYAOX010000445          395 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.451, whole genome shotgun sequence.
ACCESSION   DAYAOX010000445 DAYAOX010000000
VERSION     DAYAOX010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.451"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..>395
                     /locus_tag="ACGI57_003769"
     CDS             <1..>395
                     /locus_tag="ACGI57_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFG9718780.1"
                     /translation="HSGDSTTMYPSQNISDEIKEKIVEYTKKIALDLNVLGMVNIQFI
                     EFQNELYIIEVNPRASRTVPYISKVSGVPIVDLATKCMLGAKLKDLGYGTGVYKEPKL
                     VSVKVPVFSMSKLSKVEVSLGPEMKSTGEV"
BASE COUNT          157 a           46 c           78 g          114 t
ORIGIN      
        1 cactctggag atagtacaac aatgtatcca agtcaaaata tatctgatga aataaaagaa
       61 aagatagtag aatacactaa aaaaatagct ttagacctta atgtgcttgg tatggttaat
      121 atacagttta tagaatttca aaatgaatta tatataattg aggttaaccc tagagctagt
      181 agaacagttc cgtatataag taaagtaagt ggagtaccaa tagttgactt ggctactaag
      241 tgtatgttag gggctaaatt aaaggattta ggatatggta caggagttta caaagagcca
      301 aaattagtat cagtaaaagt accagtattc tcaatgtcta aattatctaa agttgaagta
      361 agtctaggac ctgaaatgaa gtctacagga gaagt
//
LOCUS       DAYAOX010000446          383 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.453, whole genome shotgun sequence.
ACCESSION   DAYAOX010000446 DAYAOX010000000
VERSION     DAYAOX010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.453"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            19..213
                     /locus_tag="ACGI57_003770"
     CDS             19..213
                     /locus_tag="ACGI57_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9718781.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT          152 a           33 c           53 g          145 t
ORIGIN      
        1 aataggaggg attctacaat gataaattat atggtagaaa ttatatatag cgaaaaaata
       61 agcgaagtag aaaacatgtt gaaaattgaa aatatacaat atgaatttac ttctcgtgat
      121 ggctttatat tttttggttt ttatgatatg acagacgcat atagatttga gactttatgt
      181 tcagatgaaa aaattcgatt taaaagaata taatagataa aaaagtacat atttctaaac
      241 tatttgtttt tcaaatttag gttgtgtttt aatgtagtgt tgatattata aacaatttta
      301 ataataaaaa agtagggaca ttatattaat atgctcctac tttttactca tacttattaa
      361 tattatttat caaaattacc cta
//
LOCUS       DAYAOX010000447          374 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.454, whole genome shotgun sequence.
ACCESSION   DAYAOX010000447 DAYAOX010000000
VERSION     DAYAOX010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.454"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..372)
                     /locus_tag="ACGI57_003771"
     rRNA            complement(<1..372)
                     /locus_tag="ACGI57_003771"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a          102 c           63 g          122 t
ORIGIN      
        1 aggactatta ccttctgtgg tgggtctttc caaacctctt caattacgat acctcttcgt
       61 tatgatatgt ccgcaacccc aataaagaaa actttattgg tttgggctaa tccgcgttcg
      121 ctcgccgcta cttacggaat cgaatttctt tctcttcctt caggtactta gatgtttcag
      181 ttccctgagt tcccctcatt aagctatgta ttcacttaat gatacttaga cattactcta
      241 agtgagtttc ctcattcgga aatcttcgga tcaaagttta cgtgcaactc cccgaagctt
      301 atcgcagctt atcacgtcct tcatcggctc ctagtgccaa ggcatccgcc ctgcaccctt
      361 aataacttga ccag
//
LOCUS       DAYAOX010000448          363 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.456, whole genome shotgun sequence.
ACCESSION   DAYAOX010000448 DAYAOX010000000
VERSION     DAYAOX010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.456"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            167..283
                     /gene="rrf"
                     /locus_tag="ACGI57_003772"
     rRNA            167..283
                     /gene="rrf"
                     /locus_tag="ACGI57_003772"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            290..361
                     /locus_tag="ACGI57_003773"
     tRNA            290..361
                     /locus_tag="ACGI57_003773"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Asn,seq:gtt)
BASE COUNT          116 a           61 c           78 g          108 t
ORIGIN      
        1 ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta ctaacttcaa
       61 ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact attgacattt
      121 aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg gttataatag
      181 caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta gcgctgatgg
      241 tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt ccaaggtagc
      301 tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc cacccttgga
      361 gcc
//
LOCUS       DAYAOX010000449          344 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.457, whole genome shotgun sequence.
ACCESSION   DAYAOX010000449 DAYAOX010000000
VERSION     DAYAOX010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.457"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(66..242)
                     /locus_tag="ACGI57_003774"
     CDS             complement(66..242)
                     /locus_tag="ACGI57_003774"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718782.1"
                     /translation="MFRDKMDKCTHMLTAYISNSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
BASE COUNT          135 a           59 c           33 g          117 t
ORIGIN      
        1 atattaccaa tatattaatg taaatgtaga taaaactaaa aagggttttc tagaaaaaaa
       61 tctgtctaat tatacttact taccaatacc atcacttgat gcaagcaaga ttccacaact
      121 ttttctccgt actctaatat aaaatcatct aactgtgtat ctataaaatc acaataatcg
      181 tatgaattac taatataagc agttaacata tgtgtacatt tatccatttt atctctaaac
      241 ataatgtatt tatcccccta aaaaactttc atgaaaaaca aaaagaaaat atctttttgg
      301 caactggctg acataactta ttttataagc gttagtccct atag
//
LOCUS       DAYAOX010000450          328 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.458, whole genome shotgun sequence.
ACCESSION   DAYAOX010000450 DAYAOX010000000
VERSION     DAYAOX010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.458"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(88..>328)
                     /locus_tag="ACGI57_003775"
     CDS             complement(88..>328)
                     /locus_tag="ACGI57_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9718783.1"
                     /translation="LSLFTPIMILKSILTYDKDTDKTKKISFFTVLLAYLITKIIILF
                     NVDKTHDYNMKKNLYDIIYYLIFITWELTIIIFIR"
BASE COUNT          140 a           32 c           31 g          125 t
ORIGIN      
        1 caaaaaataa atttatagat ataacatctt ttattaaagg gtgatatacg tcaaataaga
       61 aaaatgaaac tatatattta acaattccta tcttataaaa ataattatag taagttccca
      121 agtaataaat atcaaataat agattatatc atatagattt ttcttcatat tataatcatg
      181 ggtcttatca acattaaata atataatgat cttagttata agataagcta ataatacagt
      241 aaaaaaagat attttcttag tcttatctgt atctttatcg taagttaata tactttttag
      301 tatcattatt ggtgtaaaca atgataat
//
LOCUS       DAYAOX010000451          270 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.460, whole genome shotgun sequence.
ACCESSION   DAYAOX010000451 DAYAOX010000000
VERSION     DAYAOX010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.460"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
BASE COUNT          111 a           23 c           30 g          106 t
ORIGIN      
        1 aatataatat tttaaacttt acttttaaat ctgaagctaa attgtaaaat ttttaggatg
       61 gcaatattta cttaaattta gaaagtaaca taatatttaa gttgatgttg tcatcctaaa
      121 agcatgtgta cataaataaa gtaagactat tgtatcttat ataataaaga gttaataaaa
      181 tgatttttat aaatatagtt attttaaaaa actactttac atatattgga tgagtataaa
      241 aatccatagg gccttttact aaatctttat
//
LOCUS       DAYAOX010000452          247 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.462, whole genome shotgun sequence.
ACCESSION   DAYAOX010000452 DAYAOX010000000
VERSION     DAYAOX010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.462"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..185)
                     /locus_tag="ACGI57_003776"
     CDS             complement(<1..185)
                     /locus_tag="ACGI57_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFG9718784.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILI"
BASE COUNT           94 a           28 c           30 g           95 t
ORIGIN      
        1 ataataagta ttatttgtat tgcatgtttg ctaataagct gattattaga cacaatagac
       61 tcaaagttat ttatggagtt gtttttatag agtttgcata aagaatagac tggtgttgtg
      121 tatgtaattt gtaaaattat acaaataaat aaaaaaaaca tcaatttttt ttgtaaattc
      181 ctcataaaaa tttcctccca ttaaaacacc tctattttat tagcaaataa gtacatctca
      241 aaagttt
//
LOCUS       DAYAOX010000453          232 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.463, whole genome shotgun sequence.
ACCESSION   DAYAOX010000453 DAYAOX010000000
VERSION     DAYAOX010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.463"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>232)
                     /locus_tag="ACGI57_003777"
     CDS             complement(<1..>232)
                     /locus_tag="ACGI57_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HFG9718785.1"
                     /translation="NVIQKIQQLVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKA
                     TFDRRLLVGETREGVLAPIQALLDEMMKEDPE"
BASE COUNT           66 a           41 c           37 g           88 t
ORIGIN      
        1 aattctgggt cttctttcat catttcatct aataaagctt gtattggagc taatacgcct
       61 tctcttgttt ctccaactaa taatcttcta tcaaaagtag ctttgcaata atctggcact
      121 actgatgctc ctggatatgg agaagatttt atatctgtta aaactaatat tccatcacct
      181 aaaactggat gagttggagg tactaattgt tgtatctttt ggattacatt ag
//
LOCUS       DAYAOX010000454          219 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.466, whole genome shotgun sequence.
ACCESSION   DAYAOX010000454 DAYAOX010000000
VERSION     DAYAOX010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.466"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>219)
                     /locus_tag="ACGI57_003778"
     rRNA            complement(<1..>219)
                     /locus_tag="ACGI57_003778"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           42 a           64 c           47 g           66 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtccc
//
LOCUS       DAYAOX010000455          206 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.469, whole genome shotgun sequence.
ACCESSION   DAYAOX010000455 DAYAOX010000000
VERSION     DAYAOX010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.469"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..167)
                     /locus_tag="ACGI57_003779"
     rRNA            complement(<1..167)
                     /locus_tag="ACGI57_003779"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           52 a           55 c           39 g           60 t
ORIGIN      
        1 ttatcccgta ttagcatacc tttcggtatg ttatccgtgt gtacagggta ggttacccac
       61 gcgttactca cccgtccgcc gctctttacc gaagtaaatc gctcaacttg catgtgttag
      121 gcacgccgcc agcgttcatc ctgagccagg atcaaactct caaataaaag tttatcaggc
      181 tcagatacta ttgttatcaa aatatc
//
LOCUS       DAYAOX010000456          205 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.denovo.470, whole genome shotgun sequence.
ACCESSION   DAYAOX010000456 DAYAOX010000000
VERSION     DAYAOX010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.denovo.470"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
BASE COUNT           66 a           36 c           27 g           76 t
ORIGIN      
        1 atttttttgt ataaacgacc ttcaatcttt ctattataga ttaaattagt atctcaaaaa
       61 aacaataaga gaggaattaa tcctctcttt ttaatttaat tagatttggc agggcggcgt
      121 atcctgcatc tctattaact actctatagt agcgtattgg gtgccttttc caacctcaaa
      181 tctaactaat aaatatctac gtgta
//
LOCUS       DAYAOX010000457         8803 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.guided.149, whole genome shotgun sequence.
ACCESSION   DAYAOX010000457 DAYAOX010000000
VERSION     DAYAOX010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.guided.149"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            complement(114..1886)
                     /locus_tag="ACGI57_003780"
     CDS             complement(114..1886)
                     /locus_tag="ACGI57_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HFG9718786.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(1900..2829)
                     /locus_tag="ACGI57_003781"
     CDS             complement(1900..2829)
                     /locus_tag="ACGI57_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9718787.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            3050..3235
                     /locus_tag="ACGI57_003782"
     CDS             3050..3235
                     /locus_tag="ACGI57_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFG9718788.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            3228..3929
                     /locus_tag="ACGI57_003783"
     CDS             3228..3929
                     /locus_tag="ACGI57_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9718789.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            3931..4854
                     /locus_tag="ACGI57_003784"
     CDS             3931..4854
                     /locus_tag="ACGI57_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9718790.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            5011..5934
                     /locus_tag="ACGI57_003785"
     CDS             5011..5934
                     /locus_tag="ACGI57_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718791.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            5927..6664
                     /locus_tag="ACGI57_003786"
     CDS             5927..6664
                     /locus_tag="ACGI57_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718792.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            6675..7448
                     /locus_tag="ACGI57_003787"
     CDS             6675..7448
                     /locus_tag="ACGI57_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718793.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(7989..8597)
                     /locus_tag="ACGI57_003788"
     CDS             complement(7989..8597)
                     /locus_tag="ACGI57_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFG9718794.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         2907 a         1457 c         1264 g         3175 t
ORIGIN      
        1 aatcattttc tatataaatc attgtatacc ttttctgtga aaatttctac attttttata
       61 aattttattc atttttatat ttttatgtat ataaattctt ttttgtaagt ttattaattt
      121 ctaattatct tctttaaata cattgaaact acatcagaaa atctagttat atctcttgaa
      181 tatataaaat catttccata tattaatctt tcagcttcaa gttctgattc ttttcctgta
      241 aatactccta atactaatat attttgcttt ctagcttttc tcacttcatt ggctgtatct
      301 ctcaaaccaa ctatacccct ataagccttt tctcctctta atgttctctc actacttttt
      361 cctatcttta catcatttgg tcttccatcg ctgagaacta tcaggatttt attttcttca
      421 tctctattta ttagaccact acatatagat tttattgcca aaccatctct attattacca
      481 gcacaaaaat attcaaatat attttcacta ttttttatac tatcatcata atctttaaat
      541 ctttttaata ttgtatagtc taaaaaacta gaaaatccca tcactctatt tggtatgcca
      601 acttctgtta gagcatttgc aattatgtaa gcttgaattg ccacatttcc ctgatttcta
      661 ctttgagagc cacttgcatc caataatata tcaataacgt atttcccttt ttcattttct
      721 atatctttat aaaatatttt attattatta cttctaccaa ctctccaagc tctattggct
      781 aatatagtac cacaatcaga atatactcta cttttagcat tttcttctat taaaattctt
      841 gacagacttt cttttaatct ctttatttgc tgtctataca ttctagcatg ttctctaaat
      901 tttcctaaat tattttcttt ttgtcttgtt acatatttta gttgaaatac attattacac
      961 tcacttctaa gaactccatc tgtaaagtgt attctacaac cttcatgaac atttctacaa
     1021 ttttgtgtct caattttttt aatttcacct ttactcaaat aagtttttcc ataataatgt
     1081 tctatttttg aatatatctt atttgcagtg tcttcatcaa catatataac tcggtctgct
     1141 gaattgctac atttagaatc atcaaaatcc cctacacctt ctacaagcat ggatgaagat
     1201 attgaatcta tatcactttc aataatattt tcttcttcat cactataaag ttcttcatac
     1261 atgaaatcag caaatgtatc aaaatttact tctacacttt ctacattatt ttcatcaata
     1321 agactatttt cttctccatt taatatgtat tcaaaataag ttaaatatat aacatctaat
     1381 gcttttaaaa tctccataat atcttgattg gtatctatat ttcttatatc attaactatt
     1441 cttctagtca tactatctaa tgctgggtgc ttgtccatac tataaagtac aaatgtgtat
     1501 ttaattttat ccataatatt atcattgtgt attttggtaa aacgtgaaac aatatcttta
     1561 taagcattcc ttcttatgtc ttctatccct ggcctgtctt taatgacctt ttcttcaact
     1621 attgtattta atataacttg aattagacct agtataacat ctttgttgta tccttttctt
     1681 attctatttg taagatattt tttaactata tcccaatcta catactttct tctagcacct
     1741 gcaattattg cgaaatatag acctatttct tttgaagagt aatctttttc tgatatatct
     1801 atactttcat tgtaatcacc agctattgtc catgataggt tattagctct attttcaaat
     1861 tcataatcat catatatcca tttcataatc tcacctatat taaatatgac ttattggaaa
     1921 cacatctata ctctcccatt tattaggtat tctagtttta attacatctc caaccatttc
     1981 tttctcatat acatcaaaag tctttcctgt aagtcccata ttaattgcaa gagttggttt
     2041 taatcctctt cttatagtct taagagatgc cataagacca cgtaaatcaa tagctttact
     2101 agatatctct ccattttgag acttcaattg taaatctaaa aatattcctg caaagtgtat
     2161 taatttttct tcatcagcat ctgaaaattc attttttaaa atcatcatta acttatcttc
     2221 ttctatagga ggaatatcta ttaccataaa tctagatacc agtgcttcat taagttcttt
     2281 tgttcctgca tattcataat tcatagtccc aataaatctt gttgctggat gtagattgac
     2341 tctctcatac ccaggtacat caattattct tctatagtct aaagctgaat gtaccacaac
     2401 tatagcatca ttttttgcca tattaatttc atcaaacacc ccaaaacctc cattaatagc
     2461 acactcataa actgagcctc ttctaagttt aacttcattg tctataaatg tatctgtacc
     2521 tataagtgtt gaactgtctg tatttatatg aaatgatgta ttccactgag gtctattaaa
     2581 tatttcccct agattatcag caagtaagtt ttttcctgtt gcctttggtc ccgataataa
     2641 tatattttct tcttctaaaa tagctgctat acacatagac aagatatctt taccataaaa
     2701 ataagtttta gactttgtta ctctgtattc taaatctttt tctaaaccat aataatttct
     2761 aaaatacagt acatcttcaa taagtttttc atctacccct tgcaccctta gattatctat
     2821 tatatccata tttttacccc ttttatataa atagtatttg tttcatttct tatcaccatt
     2881 ttagcataaa aggatataag ctaaaattga ttttttaatc tccccatttt aagtaaaacc
     2941 atgcaccatt ttatcactac aaaattaaga gtactactta ctgatatttc ttgattttgt
     3001 tcgtctatac gtatataata tatgctgata aattatgagg aaaggatgat tgaaatatgg
     3061 atgcccagaa gtagcaaaaa aatggggtat ttctgaaaga agagtacaaa aactatgtga
     3121 agaaagtcgc ataccaggag tttcaaaact cgggtatatg tggctgatac caaagaacgc
     3181 agaaaagcct gttgatggta gaacaaaaag aagaaaggaa gtacaatatg aatagcgttt
     3241 tgattataga tgatgacaaa gaactttgtg ctcttatgaa aaaatgtgtt gagcaagaaa
     3301 atttatctgc tattactgca cattgtggtg cagaaggtct acatttggtc gataaaaata
     3361 gacacacact ctctcttgta attttggaca taatgatgcc agatatagac ggttttcaag
     3421 ttctgaaaaa aattagagaa acaagtaata ttccagtaat tatgcttact gctaaaaatg
     3481 acgaggatga taaagtttcc ggactacgtt taggtgctga cgattaccta acaaaaccgt
     3541 tcagcataaa cgaacttatg gctcgtgtca attctcttat tcgtcggtat actatgttaa
     3601 accctgcttt tataacagac acagattgca taataataaa tggcatgatt attgacaaga
     3661 caaaccgaat tgtttctgtc aataatattc ctgttcaact tacaagcaaa gaatttgatt
     3721 tacttgcttt tctggcttct aataagggac gggtgtttac caaaaaacag atttatacac
     3781 aagtatggga agaagaatat gcttttgacg atagtaacat catgtctttc atcagtaaat
     3841 tgagaaaaaa gattgaacca gaccccaacc atccatttta tattttgaca gttcgtggtg
     3901 tcggttatcg ttttaataag gaggcttaac atgaatacaa gtgtctatct gcttttgata
     3961 ttggttctta gcctgattat tatagcgtac cttgtttctg tcctgctacg tgtccgaata
     4021 caacttacac ttataaaaga tgctttagag gatataaaaa atggaaacct taatcgacgt
     4081 attttaacag atgaaaacga cattacaaaa caaatttgct atgatattaa cgaaatagca
     4141 atcaatagcc aatttcaact tattcagcaa aaacaatcag aacaagccta caaacagcta
     4201 atgacaagtc tctcccatga tgtaaaaact ccacttgctt ctttggtcgg ttatttggaa
     4261 gcagtagaaa gtaaaattgt tgtaggagag gaaaaagacg agtatatcca tgttgcgtct
     4321 aataaggctc attacttaaa gaattttgta gaaaatctat ttgaatgggt aaaattggat
     4381 tctaaggaac aagtttttca ttttgatatt tttgacttga atgaattatc ccgtaatatt
     4441 atatctgatt ggatacctgt tttagaaagc agtcattttg aatatgaatt tgatatacca
     4501 gaaatagagt acttcttacg tatagacgca aatgcatata cccgtatcat taataaccta
     4561 ttacaaaata ttattaccca tagtagtggc aacaaaatga cattgagaat ttttgaaaac
     4621 aaagagcaag ctcaaattat aataacagat aatgggaaag gtatatcttc agacaatctc
     4681 ccacatattt ttgaaaggtt gtaccaatgc gaccattcac gagcttcaaa gggaaatggt
     4741 ttaggcttgg cgattgcaaa ggaacttatt aacgctcata agggaaatat taccgccaac
     4801 agcactcccg gtatgggaac agaatttaca atattgcttc ctaaagccct gtaaaagtac
     4861 gaggctttct tactctaaaa caagataaaa gcaagattat ggcaaggttt tggcaaggtt
     4921 catgttttat aattctaatt gtagttgatt ttgacgttgt atcaactatc ttttgtagaa
     4981 acaaatttat taaaagtgag gaaattatat atgaatgatt ttgtgattga aacaaagcaa
     5041 cttacaaaaa tttatgggaa tcaaacagta gttgacaaag tcaatctcca tgcaaaaaaa
     5101 ggccgaatat atggtctgtt aggacgcaac ggagctggga aaaccacaat tatgaaaatg
     5161 attttaggat tgacttcaat cacgtatggc gaggtagatg tatttggaga gaatattaag
     5221 ggacgtgaaa aacaagtata tccccgtatc ggtgcaatta tcgaaacgcc aggattttat
     5281 cctaatctga caggaacgga gaacttggag atttttgcaa aactgcgtgg tacagccgcc
     5341 cctaattctg taaaaagagc attggaaatt gtaggcttac cttataaaaa taaaaagctg
     5401 tttagtgaat attcacttgg catgaaacag cgacttggta ttgccaacgc tatcctgcat
     5461 gacccagaac tgttagtttt agatgagcca accaatggtc ttgaccctat tggtattgcg
     5521 gagatgagaa agtttattag ggacttgagc gttgagcatg gaaagacaat tcttatttcc
     5581 agtcatattc tttcagaaat ctcattgttg gctgatgata ttggaattat cgaccacggt
     5641 gttcttttgg aagaaagtag tatgagtgaa ctagaaaaga aaaacagtaa atatatcctg
     5701 ctacaagttt cagatattcc gaagacatca cttattttag agcgtcaatt taacacgaag
     5761 gattattccg ttcaagatga ctacacgtta cacctttata acactaatct ggatatgggt
     5821 gaaatcaata aagcacttgt aatgcagaat gtaactgtta ttagttctca actttgtaat
     5881 gatactttag aggactactt taaaaaaatc acaggaggag agggcattgc ttaaacttat
     5941 tcaaacagaa tttcttaaac tgcgtcgtag gaaacttatt tggctgatgt tgttgtctgc
     6001 tctcattatg ccgtttttcg cactactata cttcaattat tttggtaaaa caggtgttga
     6061 gccaataaag ttttataaat ggtctgcatt tggttataca ctttggatta ttttgcctgt
     6121 tgttttgggt atattttgca ccatgcttat acacgaagaa aaccagtatg atatgctcaa
     6181 acagctttgg attgtaccta ttagtaaaat gggatacttt tttagtaaat ttttcgttgt
     6241 tttgatttac tctatttgtt ttatgctgat tacagccgtt gcttctgtgt tatttagtgt
     6301 actttctgga tatgtcgtat ttagctgggc aagcatttta tatttgttga aaaaatgttt
     6361 agaaattggt gtaatcactg cttttgtaat gttgccgatt ttggcaatcg ctacatctca
     6421 aaaaggatac attctcccaa tttgtatcac attggtatat gccttttccg gttttgttat
     6481 aatgacaata aatatgtatt tacacccatt atccagtatg ggagttatta tcatgagaaa
     6541 taaagacatt ccaggtctta tattttcaca ggaaattaat gtcccattag catttctttg
     6601 tatttgtatt tggagtattg tatcagtatt gtttgcaaac actgcactag gaagaagaaa
     6661 gtgaggtgta gataatgtgg aaattacttt gggctgaatg tcaaaaaatt cgtcgttcaa
     6721 aaattgtttg gattacagtt tttgcatcca ttatgatagc agtcatagtt tttgcaggag
     6781 ggcaggatgt atataatgac ccagatttac attatggctt aaagagcgtt tatgacggtt
     6841 cacggtatat agacaatgca ggttggtata tggacgcagt acaaccttgg tcaacttttt
     6901 ttgttcttcc tgctgtcatt gccctattag gaagctatat tatttgtcgt gaagaagagg
     6961 aggacactat taaatccctg cgtcttattc ccataaatga aacaaaatta accatagcaa
     7021 aaatgattat tacttttata ttcagtatac ttctttattt gttactcttt actataacat
     7081 ttttgactga atccgtttta catttttccg atttatcgac aaaactggtt ttaagttgca
     7141 tgaaagagta ttttcttgat ggcataggtg tattctttgc aatttctccc attattgcct
     7201 tggtatcgcg catgaaaaaa ggatattggc ttgcattagt attcacagaa gtttattcta
     7261 ttgctggact atttgcgggt atgtcaaatg tcttacaaac cttttttcct attgcggcaa
     7321 tatttaatat ttctggatac tatataacta ctggagaaaa aattatgggg agtatcataa
     7381 gtttattact ttgtgcctgc ctttctgcat ttatacttaa agggctaaaa cacaatgaaa
     7441 aaaattgaaa taggtatatc aaaatgaaaa agataaaaaa actgattatt ctaattgtat
     7501 tcttgtttct ttttggcatg ttacactgtt atactgaatg gaaattcaac aataacccac
     7561 ctaaaggggt aatagaaaga ataaatgaat ggactgataa gattggactg gattagtaac
     7621 ttatgttaat tatagtgaat gtaatattag caagttattg tttcgtattt taatactata
     7681 actttaaata tttggtttta atcttccaaa cgaatataaa aaaactgtca cagaggcaac
     7741 atatttttga atatttataa agtggtagtt ttgattttca aagctgccgc cttttcttct
     7801 atataagcaa acttatcctc aacaaagtgt aaaacatggt acattttaag aaaaaagtac
     7861 cacagacaca agcaataaaa gaaacgccga gaacctacaa aaacgtagtg cttatgcaga
     7921 tttacgacgc cttctatttt ctcaaccgtc ttaatgacct tgtacaaaag aaatttttct
     7981 aatatttttt ataatcttat cttgtgatgt tttttatgta taaatgactt aataacaaat
     8041 ccaataaaaa ttatggatat tggtataatt agaacgagga atttataatc attataatgt
     8101 gacattattc ttacccaact ctcagaaaaa atatatccta tactgattaa tgaagtattc
     8161 catatggtta ttcccacgat agatgatatt acaaaagtta taatattttg acctgctata
     8221 cctgctataa aagatatgta tgttctacat aaagggatta atctacatac acaaacagaa
     8281 atagaagagt atttgttaaa ataatcttga gatgcaaata ttcctttctt tgcctttgga
     8341 catatctcta ttattttatt tatgatagat ttacctcccc aatttcccac tgtataacaa
     8401 aatatcgaac caagtatacc tgcaattata cttaaaatta aaattgtcag aaatccaaaa
     8461 tgatttcttg cagcaattgc tccacataaa ggtaaaacaa cttcgctagg taatggaaaa
     8521 caagcatact caatcataat taatacaaat acagagatta atccatattg taaaataaaa
     8581 ctattgatta cagacactta cttatctatc tcctttctta tttaaaggtt ttaagggtac
     8641 atgttctaag gattctcacc ctatatttaa agaatcaatc tctttaaact attatatgta
     8701 tttacacaaa gcatttataa catactttaa aaatagtacc ttaaaataag aaacttaata
     8761 aataaaaaaa gattacgtgg ctacgtccta ctctcccagg cag
//
LOCUS       DAYAOX010000458         5036 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.guided.242, whole genome shotgun sequence.
ACCESSION   DAYAOX010000458 DAYAOX010000000
VERSION     DAYAOX010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.guided.242"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..1384
                     /locus_tag="ACGI57_003789"
     CDS             <1..1384
                     /locus_tag="ACGI57_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9718795.1"
                     /translation="EEIDKLYSYTGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSA
                     PSVCPVCGAKEDQFIEVEEDEVTFRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKIT
                     MISNEDELTYYRPALSDGINEELGSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTI
                     IVNDGAIKFDKLVIATGSRNFIPPIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVI
                     GGGLLGLEAAWEFRLKGLEVVVVEAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVA
                     VDGIEGDGKAQKVVFKDGDSVDCDMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQT
                     NVKDIYACGDVAQVGNISLAIWPSSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVK
                     VFSIGNIQNFDKEISSKDEGQRIYKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGD
                     FEDIMKADIL"
     gene            1818..2519
                     /locus_tag="ACGI57_003790"
     CDS             1818..2519
                     /locus_tag="ACGI57_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-G-Cd"
                     /protein_id="HFG9718796.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            2569..3651
                     /gene="vanS"
                     /locus_tag="ACGI57_003791"
     CDS             2569..3651
                     /gene="vanS"
                     /locus_tag="ACGI57_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFG9718797.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            3912..5012
                     /gene="vanG"
                     /locus_tag="ACGI57_003792"
     CDS             3912..5012
                     /gene="vanG"
                     /locus_tag="ACGI57_003792"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HFG9718798.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
BASE COUNT         1905 a          505 c          947 g         1679 t
ORIGIN      
        1 tgaagagatt gataaattat attcttatac aggtaaattc ctagataagt taaattctac
       61 ttttggaagt aaaaagaaaa ctaagaaatt taaatgtgta atttgtaatg aagtttttga
      121 aggtgatagt gctcctagtg tatgccctgt atgtggagct aaggaagacc aatttataga
      181 agttgaagaa gatgaagtta cttttagaaa agatactgat gaatatttcg taatagtagg
      241 aaatggagca gctggttttt atgcagcaga tgctataaga aaaagaaata aaacttgtaa
      301 gataactatg atttctaatg aagatgagtt gacttactat agacctgcac tttctgatgg
      361 tataaatgaa gaacttggtt cagatttcta catggaagat aaagattggt atgataaaaa
      421 taatatagta gtaatattag gaacgaatgt agataaatta gatgaagtga ataaaactat
      481 tatagtgaat gatggagcta ttaaatttga taagcttgta atagctacag gaagtagaaa
      541 ctttatacca ccaattaagg gccatgattt agaaaatgtg tttactctta gaaatataaa
      601 agatttatat agtgtaaaag aagcactaga aaaatctaag aaagttgtag tgattggtgg
      661 cggtttattg ggacttgaag ctgcatggga atttagacta aaaggattag aagtagtagt
      721 agttgaagcc atggatagca tactatcaaa acaacttgat aaagaaggta gtaaaatatt
      781 agaacaatgt gtaagagata caggaataga tgtaagattg ggtgtagcag ttgatggtat
      841 tgaaggtgat ggaaaagctc aaaaagttgt atttaaagat ggagatagtg tcgactgtga
      901 tatggtagta ttttcaattg gtgtaagagc aaacactcaa atggttcagg atacatctgt
      961 aaaaattgat agaggtattg ttgtagataa gacattacag acaaatgtta aagatatata
     1021 tgcttgtgga gatgttgcac aagtgggaaa tattagttta gcaatatggc catcttcagt
     1081 agaaatggga aaaatagcag gggcaaatgc aagtggagat aatttgacat ttgaaagtga
     1141 agtatatcca gtttcattag atgcaatgaa tgtaaaagta tttagtattg gtaatataca
     1201 aaattttgat aaagaaattt catcaaaaga tgaaggtcaa agaatatata aaaaattatt
     1261 tatgaaagat ggttcattag ttggagctat acttataaat gatttaagct gtactgttaa
     1321 attgataaga ttaatatctg aaaaaggtga ttttgaggat attatgaagg ctgatatact
     1381 ataatttaaa tacattaaaa taaaaaggag ttgtctcaaa attgattttt ttctaatcaa
     1441 attttaaatt caactccttt tttgaatttt tatataaaat aacatggaaa ttttagtaat
     1501 gtctgtggtt acatacatag gtttaattgt tttttatatg gattgtctct ggtataataa
     1561 attgaattgt gtttgactga tagatatttt tattgaaagt taatacctgt tatgcgataa
     1621 taaggacgtg ttaggggagt ttttataata acaattaatc tcagtttttg tgttccaata
     1681 gtagcatcaa cacattctga tgtagtagtt cttttcttat aaaaattagt tacaaatatg
     1741 cttgtaatta tttgatatgt aaatttacca atattataaa tgatgacctc gataataagg
     1801 agagtgaaaa ataatctatg aacgaaaata ttttgattgt ggatgatgaa aaagaaatag
     1861 cagatttaat tgaagtatat ttaaaaaatg aaggttttac agtatacaag ttttatacag
     1921 gtaaggaagc tctagaatgt attgaatcta aaaatttgga tatggctatt ttagatatta
     1981 tgcttcctga tattgatgga tttcatattt gccagaaaat tcgagaaaaa tactattatc
     2041 ctatcattat gttaacagct aaaattgaag atatagacaa gataatgggt ttaaccatag
     2101 gtgctgatga ttatataaca aagccattta accctttgga agttgttgca agagtaaaga
     2161 cacaattaag aagatatatg cgatataata attcttatga acaacagtcg ataattgtta
     2221 atgagtatga tattaaagga ttgattatca ataaagaaac acataaatgt agtttatttg
     2281 ggaaagaagt agcattaaca ccgattgaat tttctattct ttggtattta tgtgaacatc
     2341 aaggaaaagt ggttccttct gaagaattgt ttgaagctgt atggggagaa aaatatcttg
     2401 ataataataa cacagttatg gctcatatag ggcgattacg tgaaaaaatt catgaacctt
     2461 ctaaaaaccc gaaatttata aaaactgtat ggggagtagg ttatacaatt gaaaagtaaa
     2521 aatattaaag tcaatcctga ctttactcaa gtgagaaata aaatttttat gagaacagct
     2581 ttaatggcat ttatagccat tattttcata tggattttat attcttttat attgtatggt
     2641 aagttttcta tctggatagt atcattcttt gaaaatgctt ttcgtatgga atataaagct
     2701 gcattgaatt tatatcagca gattttccgt aatagcatgg agctcttcat gatactagct
     2761 atggtgattg tgttttttat aatattccgg gtttatttaa attggtttac aaaatatttt
     2821 agtgaaataa atcaaggtat tgactctctt atcaaagaag atgttggaga agtggcttta
     2881 tctccagagt tattggcaat agaaaagaaa ataaactcaa ttaaacatat acttgaacaa
     2941 cgtaaatttg agacacaaat ggcagaacaa cgtaagaatg aacttattgt ttatcttgca
     3001 catgatttaa aaacaccact tacatcagtt atagggtatc tgacactact tcgagatgaa
     3061 agtcagattt ctgaagaact gcgaaaaaaa tatttatcta tttcgttgga taaggcagag
     3121 cgtcttgaag atttaattaa tgaattcttt gaaattacaa ggtttaattt atcaaatata
     3181 tcacttgaat acagtacagt aagcttaact cgtatgcttg aacaactaac atatgagttt
     3241 aaacctactt tgatgagtaa gaatttaaaa tatacactga atgttacacc aaatatgatg
     3301 ataaggtgtg atgttaataa aatgcaacgt gtatttgata acttattacg aaatgctgtt
     3361 tattatagtt ttgaagatac tactattgaa ataacagctg tacaggagga tgattatata
     3421 aagcttaaat ttataaatca tagcaatacg attccagagg aaaaacttga gcgtattttt
     3481 gaacaatttt acaggttgga tacatctcgt aattcaaaca gtggtagagc tgggttggga
     3541 cttgctatag ctaaagaaat agtggagctt cataacggga caataacagc tcatagcgag
     3601 aacaatataa ttatctttaa ggttattatt ccgattgtag gaaatttgta agaatttaag
     3661 tataaaaaaa taggaaattc tttaggataa ataaataaag agcactcttg ttattgatat
     3721 aattactgta tcaaaacaag agtgctttta ttttgataca gtaattatat caatagaaaa
     3781 atgtgaaaat atattatgtg gaacagatgc aattgtaagt gaaggttgat tggatttgaa
     3841 atagaaaaag tattttaaca tgtttctgta gattgtataa aaataaataa atcaacaaag
     3901 gagaatcaaa gatgaataat aaaaaaattg cagttatatt tggtggaaac tcaacagaat
     3961 atgatgtatc attacaatca gcttttgcag tacttgagaa tttgaatacg gaaaaatatg
     4021 atattatacc aattggaatt acaagggagg gtaattggta tcattatatt ggagattata
     4081 ataatatcca gaataacact tggtttgaaa gggatactga tataattcca gttattgttt
     4141 cgcagaaccg tgtcaaaaat ggtattattg aatttttaat tcctgatgct gtttctcaga
     4201 atcatattcc aaatagtgct attgaatcat gtgaaaatga tattaaatac actaaacttg
     4261 attttgtgtt tcctgtactg catggaaaaa atggagaaga tggaacagtg caggggttat
     4321 ttgaattggc aggtatacag gttataggat gtggtactct ttcttcagca ttatgtatgg
     4381 acaaagacaa atcacataaa cttgttcatt cagaaggtat taaagtacca aaatcaatat
     4441 tactaaaata ctcagataaa tttaatgtgg ctgaactgtc aaaagactta acttatccat
     4501 tatttataaa acctgtgcgt gcaggttcat catttggtat tagtaaaatt tataaaaaag
     4561 aagagttaca agtagcagta gacttggctt ttgaacatga taatgaagta ataattgagg
     4621 aaaatattga tggttttgag gtagggtgtg cagttttagg aaacgatgac ttacttgttg
     4681 gaagagtaga tgaaatagaa ttgtcaaaag gattttttga ttatacagaa aaatatacat
     4741 tgaaaagttc aaaaattcat atgccagcta gaattgattt ggattgtgag aaaagaatac
     4801 aagagacagc aaaaattatt tatagaactc ttggatgttc tggatttgct agagtagata
     4861 tgtttttaac tccatctggt gaaattattt ttaatgaagt caatacaatt cctggcttta
     4921 cttctcatag tcgttatcca aatatgatga aaggtattgg attgtctttt gaagaaatat
     4981 tggacaagat aattgaggtg tacataaaat gatgaataat attttattag ataaaa
//
LOCUS       DAYAOX010000459         4804 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.guided.251, whole genome shotgun sequence.
ACCESSION   DAYAOX010000459 DAYAOX010000000
VERSION     DAYAOX010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.guided.251"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            343..1035
                     /locus_tag="ACGI57_003793"
     CDS             343..1035
                     /locus_tag="ACGI57_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9718799.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            1032..1967
                     /locus_tag="ACGI57_003794"
     CDS             1032..1967
                     /locus_tag="ACGI57_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9718800.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            2115..3038
                     /locus_tag="ACGI57_003795"
     CDS             2115..3038
                     /locus_tag="ACGI57_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9718801.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            3022..3792
                     /locus_tag="ACGI57_003796"
     CDS             3022..3792
                     /locus_tag="ACGI57_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718802.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            3802..4584
                     /locus_tag="ACGI57_003797"
     CDS             3802..4584
                     /locus_tag="ACGI57_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9718803.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
BASE COUNT         1516 a          728 c          940 g         1620 t
ORIGIN      
        1 tttctggaat aattgcttat ttcacatatg ttttattgtg atgttttgat ttaataaatt
       61 cttcaactct gtatatagta aatgtattat catgtttgtt ttattcaagc acacttataa
      121 tacgcttgat tttttcacaa tgatgaaata tactaatggg tgttataggt ataagaatcg
      181 cattacactt gaagaagtaa gtgataaatg aggtataaat cctcatatga taaactacta
      241 tttcattgag gaacatattc taagtgatgt caagatacta caatatgact aaattcctag
      301 tgataaaaaa gtctacgaat gagtgtagaa aggggaacta agatgaataa agttttaatt
      361 atagatgatg ataaagatct ttgtattttg ttaaaaaaga gtatttcaat tgaacagatt
      421 gaagcaaact attgccattc tgggagagat ggactggttg aactttcaaa acaagattat
      481 cagttagtag ttctagatgt gatgatgcca ggtatggatg gttttgaaac tatggaacaa
      541 atacgaaaat atagcagtat tcccatctta atgctgacat caaaaagcga taattacagc
      601 aaagtgcatg gattgcgagc tggtgcagat gactatctaa ccaaaccatt tgaaattaca
      661 gaatttactg ctcgtgtact gtctttaatc cgacgttaca cccgttttaa tccaaaaaat
      721 gaggagccac agcaactttc tttcaaaggt ttgttgattg accttgacca gcatacagtt
      781 actaccaatg gcgaacctat gttgcttcca cccaaggaat ttggtgtact tctttactgt
      841 gcaaggcatc agggtaagat tttgacaaaa cagcagattt atgaagccgt gtggggtgag
      901 ccatatgtat atgatgacag caatatcatg gctattatca gtcgcctaag aaaaaaaatt
      961 gaactagacc catcatctcc catctatttg caaacaataa aaggaattgg gtatcaattc
     1021 aacacggagg tatgaaaatg gatgaacaaa cgataattct tttcttactc ttgctgtcga
     1081 ttggtctact ttttctttcc atatttgctt tttccaagct tgggcatatt tacaagcgcc
     1141 taaaagatat agaggagata ctggctgatg tacaaattgg agacgaaaat cgtaaaatac
     1201 taattaagcc atgtgatgtt atggctccat tggtttatca gcttaatgag attgtctacg
     1261 attatgaaaa taagttactt tctctgaaaa agtcggataa agctagcaaa caacttatga
     1321 caagtttatc tcatgatgtt cgtacaccat taacaactct gattggttat ctggatgctg
     1381 tacacagtgg tatagttatc gaacaagagc gggaggaata ccttgaaatc gctaggcgta
     1441 gagcctacga cctcaaggat tacattgatg ttctttttga ctggtttcgg ttgaactctg
     1501 atgaatttac gctgtcaata gaatctgtgg aaattgcaga gttgtcacgc aatattttga
     1561 aagactggat tcctatattc catgaaaaga aactgaattt tgaaattgat attccagaaa
     1621 atcgactaat ggtaaacctc gacccagatg gatattcccg tgtggtcaat aatctggttc
     1681 aaaatgttct tgctcacagt aaggctcggc aaatcaagat taccatgagt gaggattcca
     1741 gaatggtatt actccgtgtt gaggatgatg gtgttgggat tgccagagaa aatttaccac
     1801 atatttttga gcgactgtat aagtgtgata aaggacgttc agaaaaagga agtggtcttg
     1861 gtctatctat tgtcagtcaa atggtggagc gaatgggtgg tcaggtttcc gtagaaagtg
     1921 agattggaaa gtataccgta tttacagtga gcttaccatt aatataaaat tgtttttccc
     1981 ccatgtgaca tgtatacaca tggggatttt ttgtaagtta taaagttgca aggttaatac
     2041 aaggttgttg caaggttaat gtaagattga ggtggtatga taactttaat aaacaaagga
     2101 ggcttttatt atttatgaat aatttaatta ttgaaacaaa aaatctcact aagcaatatg
     2161 gtgctcaaaa gagcgtttct aatttgaata ttcacgttca gaaaggtcgc atttacggat
     2221 tgctaggaag aaacggtgcg ggtaagacga caactatgaa aatgttattg ggtctaacaa
     2281 aaccaactac tggagaaatt aaaatatttg gtcaggacat tcacaataac gagaagaagc
     2341 ttcttcctcg aattggatgt ttgattgaat caccagggtt ttatcctaat ctgacaggaa
     2401 cagaaaatct caaaatcttt gcaaggctta gaggaatacc aaaaaaaaat gcagtagaat
     2461 cagcactgga tgtagtcggt ttgccaaaca atgacaaaaa gttattttct caatactctc
     2521 ttggtatgaa acagaggttg gcgattgctc ttgccattat gcatgaccca caactgttga
     2581 ttttggacga gcctatcaat ggacttgatc ctattggtat tgcagaagta agaacgttta
     2641 ttcgtgatct ctgcacagaa cgaggtaaaa ctattttgat ttccagtcac attctttctg
     2701 aaattgcctt gttggcagat gacattggca ttattgacca tggtattttg ttggaggaag
     2761 aaagtcttgc ccaattagaa gaaaaaaaca gtaaatacat acattttgct gtatctgatg
     2821 cagcacaggc agcaaggctt ctggaaaccc aacttaacct gaaaaatttc aaagtagcag
     2881 ataattacaa tattatgcta tatgatacag atgtttctgt tgcagaaata aatcgtgctt
     2941 tcattctaaa caacattgag gtatcagaat ctcatttatg cgaagatact cttgaagatt
     3001 acttcaagaa agtaacaggg ggtgaaggaa ttgcttgata taatttcatg tgaattttca
     3061 aaactcaagc ggttaaaatt tatactgatt tctattttag gtgcttgctt atttccaatt
     3121 cccgcaacaa ttttgattgc aaaggacaac ttgccctttg agcaattgtt caaattagtt
     3181 gtcaattttg gatatttttt gctattacca attgtattaa gtattgttgc tagccaacta
     3241 ttttttatag aaagagataa tgatgtatta aaaaatcttg caacagtgcc agtgcaaaaa
     3301 ggaaaactgg ctctggcaaa attggctgtg ttgctgttta tatcattgtt ctactctgtt
     3361 gcagggttag gagctactat cattggaggt ttcattgttg gaatagtaga gggggttgct
     3421 gtaaaattag gaatgagcat tgcttttgga attatgtttt ttgttactgt acttcctgtt
     3481 gtggtactca ttgtatattt caatcgcagc tatatttttt ctattatact ttcttttgta
     3541 tatgctattt tcaattttag tatatcctta aatattatca attttgaacc aaataaccca
     3601 atattaagtg tattacctgc tcctgttata atgcgttggt ggatggcatt ttggggagat
     3661 cccactggcg aatacacggc acttagacag ccatatttgc tctcaactcc tgcttgtgta
     3721 ggtattttat gtttgataac cattatagca gtcctgctga tttgtatgat atacaaaaag
     3781 caggagaatt aggaggtatc tatgcttgat ataattacta cagaatttca aaaaataaaa
     3841 agatataata ttctatggat tggtgttgtt gctgttctgt tttcagcact attggcagtt
     3901 tttcaggttg taagttcaca cggcagtgac cctctaacgt atgagagctt tgcaaacgga
     3961 gttatctgga ataatttcag tcttagtttt ccatttggga ttacacttat tggaggatat
     4021 cttattaacc gtgaatatac agaccaaacc ttaaaaaaca tcctaactgt tcctatttca
     4081 ttacgcaaat tgctagtggg aaaattaatt gcggttggag gaattactgt attgttaagt
     4141 cttttcagct tcttatgcac atttattttg gggttgttgt tttgcaatat caatatatct
     4201 gttacactcg ttgtaaaatc cttagttcag attttgtctg ttaatctgta ttgttatgtt
     4261 gctgttttgc cgattattgc ttattttggt cgtaaacaaa atgcatttct gacgggtgta
     4321 gggattgcat ttgtatacgg tttttgtggt gtgtttgttg ctggaagaaa tctgacagac
     4381 ttttatccaa tcacagcagg tcttggtcta gttggataca acaatggtgc tggtactgtt
     4441 tatcagccac ttatcggtgc tatgactttg attgtaattc taatcttgac aactattcta
     4501 cttgtattta cacctaatta tgataaagtt atgaccatat ctaagaaaaa agggataaag
     4561 cataaaaagt attctcataa ataacataaa tattgtttct attatttaag ttgctagaca
     4621 gtgaaaagaa aaaatctgtc gtacagtgtc tatatgctca tgtattcaag aagctacagt
     4681 tttgattttt cataactgta gcttcttttt gtatttatta aatcacgact atcataatac
     4741 ttgcaatagg tatagcgaca agagagagta ctgtcatatg tgttttatat attacattaa
     4801 gtat
//
LOCUS       DAYAOX010000460         3116 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.guided.308, whole genome shotgun sequence.
ACCESSION   DAYAOX010000460 DAYAOX010000000
VERSION     DAYAOX010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.guided.308"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            <1..782
                     /locus_tag="ACGI57_003798"
     CDS             <1..782
                     /locus_tag="ACGI57_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFG9718804.1"
                     /translation="NKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSEDIDLIPVDT
                     RFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLRDNGKDFTKK
                     FVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYGFIERYQCKK
                     EEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKIEDKEKIIEI
                     FYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            800..2938
                     /gene="vanT"
                     /locus_tag="ACGI57_003799"
     CDS             800..2938
                     /gene="vanT"
                     /locus_tag="ACGI57_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HFG9718805.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         1116 a          364 c          523 g         1113 t
ORIGIN      
        1 aaaataaaga agataaaatg acaaatacta tcttattgac taaagataaa gtttatgatg
       61 ggaatctaat tctagtaaat gcttttttac cagttaaaac atctgaagat atagatttaa
      121 ttcctgtaga tacaaggttt ccaagtattc ttatgaaacg tgaagctaca aacattttac
      181 aaaatatttt aaaaagtata tgtggaataa atgaaatagt acctgtaagt ggatatcgta
      241 ctgcagagga acaacaagat atatattcaa gttctctgcg tgacaatgga aaagacttta
      301 ctaaaaagtt tgtagcactt ccaaatcaca gtgaacatca gacagggctt gctattgatt
      361 tagggttaaa gaaagaagtt atagatttta tctgccctga atttccttat aatggtattt
      421 gtaatgagtt tcgtaaggca gcactacatt atggttttat agagcgttat cagtgtaaaa
      481 aagaagaaat aacaggaata tctcaagaac catggcattt tcgttatgtg ggatatccgc
      541 attctgagat tatcaataaa atgaatctcg ctcttgaaga atatatagaa tatattaaga
      601 gattttcgta tacaaattgt ttaaaaatag aagataaaga aaaaattata gaaatctttt
      661 atgtgcctat tcatactaaa gaagaagtga gaatctctat acctgaacaa tgtgtatatc
      721 aaatatcggg taacaataag gatggtttta ttgtgacatt ttggagaaat agaaaggact
      781 aagaaatgga ggaacaagaa tgattaggaa taaatcttat gcagggcttg attattttag
      841 gattatagct gcattactta taattgcaat tcatacatct ccattattat catatagtaa
      901 aatggctgac tttgtgttaa ctagtataat tgctcgtatt gctgtgccat tcttttttat
      961 gctatcaggc ttttttctaa tttcaacata taattataat tgtaataaac tgaaatcttt
     1021 tctaaaaaat gtattattta tttacgggat agctattgta atttatattc ccttaaatat
     1081 ttacactgga tatttttcca tgaataattt agtgcccaat attattaagg acattgtatt
     1141 tgatgggact ttatatcatt tatggtatct acctgcatca attattggtg caagtattgc
     1201 atactttctt gtaaaaagac taggtttaaa gtactcacta gtaatcacta ttgttattta
     1261 tgtaattggt atgtttggtg atagttatta tggtttttta gaaaaaatac cactattaaa
     1321 gaatttatat gggtatattt ttgaagtatc agattataca cgaaatggta tattttttgc
     1381 acctgtattt ttcatacttg gtggaataat atctaataaa tctaattata tgtcaactaa
     1441 aaatagcttg attggatttc ttatatcatt cttacttatg ttatgtgagg gaatgttttt
     1501 acataaacta ggggtacagc gtcatgatag catgtatatt atgttattgc catgtatgta
     1561 tttcttattt acagccttaa catttttgaa aggttctcga gctaggttat tgcgaacatc
     1621 atcattaatt atttatatta tacatcctat gatgattgtt gttattcgta tgttttctaa
     1681 aatattcgga atacaaactg tcttggttga caatagcctt attcattata tagtggtaag
     1741 tatagcatct tttatagctt ctataattat tatattgatt cttaaacctc taaaaaaaga
     1801 cttgaaagga gaacataagg taaataccaa tagagcttgg attgaaattg atttaaataa
     1861 tttggaacat aatgtaaaga tgatacaaaa agctatgcca gaagattgtg aattgatggc
     1921 agttgtaaaa gctaatgcct atggtcatag tgcttttgaa gtagcgactt gtgcaaacca
     1981 aataggtgta aaagcatttg cagtagcaac aatagatgag gggattgatt tacgtcgata
     2041 tggaatacta ggagaaatat tgattcttgg atatactaac ccaataaggg caaaggaact
     2101 tcataaatac aatcttacac agagtgttat tgaccttaat catgctatct ctttaaacaa
     2161 acagaaatac aacataaaat cacacattaa aattgataca ggtatgcaca gactgggttt
     2221 tgatgttaat gatgtgaggt caattttaaa ggcatttaaa ttaaaatact tagatatatg
     2281 tggcatattt acacacttgt gtgtttcaga tagtctgaaa gatgaagatg tagactttac
     2341 aaataaacaa attgaatgtt tttataagtt aattgatttt ctgttaaaaa aaggtataaa
     2401 aattcctaaa gtacatattc aaagtagtta tggttttttt aattatccca atcttaagtg
     2461 taattatgta agaattggaa ttgcattata cgggatatta agttctccaa atgatacgac
     2521 taaattgcaa cttgatttac gacctgtttt atccttaaaa tcacaagtaa ttcttattag
     2581 aaaaatacaa aaagatgata gttttggata tggaagagtc tttattgcaa gcagagatag
     2641 cgtaattgct atattatcta ttggctatgc cgatggtata cctagaaatt tatcctgtgg
     2701 aaaaagttac gtaataataa atggttgtag agcagcaata gtgggtcgaa tttgcatgga
     2761 ccaacttgct gttgatatta cagatatacc taatgtagaa gttggtaata cggcaataat
     2821 aataggaaga gataatttat ctgaattatc tgcttctgaa gttgcaaata actcatgtag
     2881 tatatcgaat gaattattga gcagagtagg aagaaggtta aatgttataa agaagtaact
     2941 caattaaata gtaatatttg ttgtactgtg taatttattt tactctgact agacattttt
     3001 agtaatatta tgtctaatat atatttttta taacttacat gacaatttaa gttataaaaa
     3061 ggtgatgttt cagattttta ttttgaaaca tcaccttttt tgttataata tataat
//
LOCUS       DAYAOX010000461         2516 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.guided.331, whole genome shotgun sequence.
ACCESSION   DAYAOX010000461 DAYAOX010000000
VERSION     DAYAOX010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.guided.331"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            190..543
                     /locus_tag="ACGI57_003800"
     CDS             190..543
                     /locus_tag="ACGI57_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFG9718806.1"
                     /translation="MRNYARDIYDENKRRNDYLVSLESLSLAELSKLSILDDYDSNYI
                     CMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMTNADIATLMDLAENTIHYH
                     KTNALKELKELMQEH"
     gene            545..775
                     /locus_tag="ACGI57_003801"
     CDS             545..775
                     /locus_tag="ACGI57_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9718807.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            1165..1368
                     /locus_tag="ACGI57_003802"
     CDS             1165..1368
                     /locus_tag="ACGI57_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFG9718808.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            1446..1778
                     /locus_tag="ACGI57_003803"
                     /pseudo
     CDS             1446..1778
                     /locus_tag="ACGI57_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            1871..2503
                     /locus_tag="ACGI57_003804"
     CDS             1871..2503
                     /locus_tag="ACGI57_003804"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFG9718809.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT          951 a          360 c          436 g          769 t
ORIGIN      
        1 catttttgcc ctataggcag agtgtgtatt tttttctctt ttttttgaaa tttatcgaag
       61 atttaacgcc ctcccaccgt gataggagta agcaaggtta tttaactcct tgcgatggaa
      121 agagggtgaa atcttatggt tgatgaaaag acaattgaac atcaattcga cagtttctgt
      181 aaaacagttt tgcgtaacta tgctagagat atttatgacg aaaataaacg cagaaatgat
      241 tatttggtat ctctcgaatc tttgtcactt gcagaattaa gtaaattgag tattttagat
      301 gattatgatt ctaactacat ctgtatggtt tcttacgact acaacatacg aattgaagat
      361 gtattaatgg cgcaagcaat cggaaaactt acaaaaagaa aacaagatat aattctactt
      421 tcattctttt tcaacatgac aaatgctgac atagcaacat tgatggattt agcagaaaat
      481 accatacatt atcacaaaac aaatgcacta aaagagttga aagaactcat gcaggaacat
      541 tgaaatgaaa cctactaaag aagtattacc ttttacaatt attttacttg ctgtacatgg
      601 cgatgtaaat gcaataaacg taatacttaa acattttgaa cactatatca ttcgtctttc
      661 acagaaaact ttatttgatg aatacggaaa tccctatatt catgtagaag aagaaacaaa
      721 gcgaatgcta ggaacaaaac taatcactgc tattttgaga tttaatttgt cttaatctcg
      781 ccctctctcc atgtatcaag ctatttggta catggagaat cactaattat ttgtctataa
      841 aagcccacaa gctattgtga ttttttatag gcaaatcaat gccgactgtt ctttgacaac
      901 tgaataaagt agatagatac gtttgtgata cagatagcca acaaatacta tacgccatga
      961 ccttaatcaa gagagctact atatagtgat ttgtgaataa cctttggaac ggttgttttg
     1021 ccgtaacctg taccacagta taatgataca cctgtatgac acggttctgt ccataagaat
     1081 gggattggtt gaaataccaa tggagcttat caaagccatc tatctacttc tgaactacga
     1141 acatagaaag gagtgataca ttatatgaac aatactgatg tacctatttg ggaaaaatat
     1201 accttgacga ttgaagaagc gtcaaaatac tttcgtattg gagagaaaaa attacgtaaa
     1261 cgtgctgatg aaaaccttga ttctggctgg gtcattatga atagcaatcg cattcaaata
     1321 aagagaaaac aatttgagaa aatcgttgat acacttgacg ttatctaatg aaattgagcc
     1381 ttatatgtta taatattcat atgacgtatc aagctcaatt cggttattga gaggagcttc
     1441 ttatcatgtc aactgaaaaa agacgagata ataaagatag aattttaaga actggagaga
     1501 gccagagaaa agatggaaga tacgcatata agtatgtata ttcatttggt aaaccacaat
     1561 ttgtttactc ttggaaactt gtacctactg ataaaccccc aaagggaaaa cgtgatgata
     1621 tatcattaag ggataaggaa aaagcaatac aaaatgacct tgatgatagc attgacacta
     1681 ttggtaagaa aatgacagtt tgtcagttat acgctaaaaa gaatggacta agaaagaata
     1741 tccgacttaa cactaaaaaa ggacgccatt atcttatgaa gattcttaac aaggagtgat
     1801 tctgtgaaga gatgtattgt agtagtggaa tagtcaaaaa cataatatat attaaaggag
     1861 agatttaaat atgactattc caagttcaaa taaggtatat ccaagaagta atgattatca
     1921 aactgtatat cttaaaaatg taataacaag ggaaaatata gaagttggag attacacaat
     1981 atataatgac ttttataatg acccaagaga ttttgagaaa aacaatgtat tgtatcatta
     2041 ccctataaac aatgataaat taataattgg taagttttgc tcaatagcat gtaatgcaaa
     2101 atttctaatg actagtggaa atcactcaat gaaatcaaaa tccacatata cttttcccat
     2161 cttttatgaa gaatggaaac tcgatatcaa ccatataacg gacgcttggg acaataaggg
     2221 taacattata attggaaatg atgtgtggat aggttatgat tctataatta tgtcaggtgt
     2281 aaatattggt gatggtgcaa ttattggaac aagagcagta gttactaatg acgttccacc
     2341 gtattctatt gttggaggag tacctgcaaa aattataaaa aagagattta ataatgatac
     2401 aatttcaaaa ttattagaaa ttaaatggtg ggattggcca tataaaaaaa ttcaatcaaa
     2461 cattaagcat attcaatcag gaaatattga taaattagtt taatatttta taattt
//
LOCUS       DAYAOX010000462         1273 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h149-20
            SAMN40601854-rid21577093.guided.383, whole genome shotgun sequence.
ACCESSION   DAYAOX010000462 DAYAOX010000000
VERSION     DAYAOX010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601854
            Sequence Read Archive: SRR28438785
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157541.1
            Genome Coverage       :: 219x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:21:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,805
            CDSs (total)                      :: 3,746
            Genes (coding)                    :: 3,718
            CDSs (with protein)               :: 3,718
            Genes (RNA)                       :: 59
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 7 of 28
            Pseudo Genes (incomplete)         :: 21 of 28
            Pseudo Genes (internal stop)      :: 9 of 28
            Pseudo Genes (multiple problems)  :: 7 of 28
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601854-rid21577093.guided.383"
                     /isolate="ST8-SSI-h149-20"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2020"
                     /collected_by="Statens Serum Institute"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="ACGI57_003805"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="ACGI57_003805"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HFG9718810.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          544 a          100 c          221 g          408 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacttaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaataa taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taa
//
